LOCUS       DASUBI010000001         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_449540, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000001 DASUBI010000000
VERSION     DASUBI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449540"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..864)
                     /locus_tag="VFU72_00005"
     CDS             complement(37..864)
                     /locus_tag="VFU72_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU5421899.1"
                     /translation="MRVNHVARRLKAGEPSVGTWLSLPSPEDAEYVSKLGFDWLVVDT
                     EHNPIDIRTLAQMFVAMSAAPVAPMVRIPWNTPENFKRVLDAGAWGVVVPMVNSREDA
                     ERAVAAARYHPAGGRSVGGGRHAMSFGTSAGDYHQHANDEVLLVAQIEHIQGVEHADE
                     ILSVPGIDACFIGPNDLAASMGLGLGVPLEFDHPRVVEAVAEVRDAARRNGVAPGIHC
                     SNAEAINQRLAEGFQFCAMASELRYMIAGLTADIARLNWQSAARAAQAAAPGETVRY"
     gene            complement(932..1819)
                     /locus_tag="VFU72_00010"
     CDS             complement(932..1819)
                     /locus_tag="VFU72_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5421900.1"
                     /translation="MSAPPDPLEIVAALGVHDARAATPITEGLGDTGLWRVERGSSSA
                     VLRLFPAGWERVAEREMLAQVAAAEAGVPVPAIEAQGGWNGHPALLLAWLPGTTLLQA
                     LAVRPGQARALGLRFGRMQARLHRARPPERLRALSFDWLDWGQPDDRLRAALHACSRQ
                     HDALLHLDYHPMNLMVLPGRTSGVLDWSNATLGEPRADVARTLTILTLLPIPGWGEAG
                     RAGVRRRFVRGYLHGYQQAAGPLGDLNLFLAWAGAAMVRDLEPKLGRAGDWLTPEALD
                     GVRRAVARWREQAGLPTFD"
     gene            complement(1816..>2527)
                     /locus_tag="VFU72_00015"
     CDS             complement(1816..>2527)
                     /locus_tag="VFU72_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421901.1"
                     /translation="RNLQYDIARSETAFLPSNRWTGRLAELDDAIQQAGADLAALRPT
                     PAVTPPLQLPEQPLEIVDVSPREPAAVRLRVGEAPFLYREELDWAERGHQLALPQLTL
                     AEGEVERLVPAGIEGAERIRLASHLENGLAIVANEALERAVDGETPPGVTLADLARPC
                     PACGGWRDVKGRCPACAALERRRDEIREALGRLIKERDDVGRELARARDRLPVFRRQL
                     IDVEQDIEKLRAKGVEPL"
BASE COUNT          335 a          974 c          880 g          338 t
ORIGIN      
        1 aggtgcccat cgatgagcag catcgtcgct cccgctctag tagcgcacgg tctcgccggg
       61 cgcggccgcc tgggcggcgc gggcggcgga ctgccagttg aggcgcgcga tgtcggcggt
      121 cagcccggcg atcatgtagc gcagctcgct ggccatggcg cagaactgga agccctcggc
      181 gaggcgctgg ttgatcgcct cggcgttgga gcagtggatg ccgggcgcga ccccgttgcg
      241 ccgcgccgcg tcgcgcacct cggcgaccgc ctccaccacg cgcgggtggt cgaactccag
      301 cggcacgccc agccccaggc ccatcgaggc cgccaggtcg ttcgggccga tgaagcaggc
      361 atcgatcccc ggcacgctca ggatctcgtc ggcgtgctcc accccctgaa tatgctcgat
      421 ctgcgccacc agcaggacct catcgttcgc gtgctggtgg taatcgccgg cgctggtgcc
      481 gaagctcatg gcgtgccgcc caccgccgac gctgcgtccg cccgccgggt gatagcgcgc
      541 cgccgcgacc gcgcgctcgg cgtcctcgcg gctgttgacc atcggcacca ccacgcccca
      601 ggcgccggca tccagcacgc gcttgaagtt ctccggggtg ttccagggga tgcgcaccat
      661 cggcgccacc ggcgccgcgc tcatcgccac gaacatctgg gccagggtac ggatgtcgat
      721 cgggttgtgc tcggtgtcga ccaccagcca gtcgaagccg agcttcgaga cgtactcggc
      781 atcctccggg ctgggcagcg agagccaggt cccgaccgac ggctcgcccg ccttgagtcg
      841 cctggcaacg tggttcacgc gcattattgt cctctttccg ttggcaatct agcgtaccgg
      901 ccgcgcccgg ccattgtagc gcgcccgacc ctcaatcgaa cgtcggcagg cccgcctgct
      961 cgcgccagcg cgccaccgcc cggcgcacgc cgtcgagcgc ctccggcgtg agccagtcac
     1021 ccgcacggcc gagcttcggt tcgaggtcgc gcaccatggc cgccccggcc caggccagga
     1081 agagattcag gtctccgagc gggccagccg cctgctggta gccgtgcagg tagccgcgca
     1141 cgaagcggcg gcgcacgccc gcgcgcccgg cctcgcccca accggggatg gggagcagcg
     1201 tcaggatcgt cagcgtgcgc gccacgtcgg cgcgcggctc gcccagggtg gcgttggacc
     1261 agtcgagcac gccgctggtc cgtccgggca ggaccatcag gttcatcgga tggtagtcga
     1321 ggtgcagcag cgcatcatgc tgccgggagc aggcatgcag cgccgcccgc agcctgtcgt
     1381 ccggctggcc ccagtcgagc cagtcgaagc tcagcgcgcg cagccgctct ggcggacggg
     1441 cgcggtgcag gcgcgcctgc atccggccga agcgcagccc gagcgcccgc gcctgccccg
     1501 gccgcaccgc gagcgcctgg agcaacgtcg tgcccggcag ccaggcgagc aggagcgccg
     1561 gatggccatt ccacccgccc tgcgcctcga tcgccggcac cggcacgccg gcctccgccg
     1621 ccgccacctg cgccagcatc tcgcgctcgg ccacgcgctc ccagccagcc gggaagaggc
     1681 gcagcaccgc gctggacgag ccgcgctcga cacgccagag accggtatcg cccaggccct
     1741 cagtgatcgg agtggctgcg cgagcgtcgt ggaccccgag cgccgctacg atctccagcg
     1801 gatcgggcgg tgcgctcaaa ggggctcgac tcccttggcc cgcagcttct cgatgtcttg
     1861 ctccacatcg atcaattggc gccggaagac cggcagccga tcgcgcgccc gcgccagctc
     1921 ccgcccgacg tcgtcgcgct ccttgatcag ccgccccagc gcctcgcgga tctcgtcgcg
     1981 ccgccgctcc agcgcggcgc aggccgggca gcgccccttg acatcccgcc agccgccgca
     2041 cgcggggcag ggccgtgcca ggtcggccag cgtgacgccc ggcggcgtct cgccgtcgac
     2101 cgcgcgctcc aacgcctcat tggccacgat cgcgagcccg ttctccaggt ggctcgccag
     2161 ccggatacgc tcggcgccct cgatcccggc ggggaccagc cgctcgacct cgccctccgc
     2221 gagcgtgagc tgtggcaggg ccaactggtg gccgcgctcg gcccagtcga gctcctcgcg
     2281 gtagaggaag ggggcttcgc ccacgcgcag gcgcaccgcc gccggctcgc gcggcgacac
     2341 gtcgacgatc tcgagcggct gctcggggag ctggagcggc ggcgtgacgg ccggggtggg
     2401 ccgcagcgcc gccaggtcgg ccccggcctg ctggatggcg tcgtccagct cggccagccg
     2461 cccggtccag cggttgctgg gcaggaaggc cgtctccgac cgcgcgatgt catattgcag
     2521 gttgcgc
//
LOCUS       DASUBI010000002         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1117462, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000002 DASUBI010000000
VERSION     DASUBI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117462"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /gene="ilvB"
                     /locus_tag="VFU72_00020"
     CDS             <1..1635
                     /gene="ilvB"
                     /locus_tag="VFU72_00020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEU5421902.1"
                     /translation="VDTIFGYPGGVVIPLYDVLPEFGVHHVLVRHEQGAAHAADGYAR
                     ATGKVGVCLATSGPGATNLVTGIATAMLDSVPIVAITGQVSQAVIGSDAFQEIDITGI
                     TLPITKHNFIIRRVEDIGPTIQKAFHLAGSGRPGPVLVDIPKDILLGKGVLAPSNAVN
                     LPGYKPTVVPHRRQVRRAAELIQQAERPLILAGHGILISGASDELVRFAEQAQIPVGL
                     TLLGLSGFPASHPLCLGMVGMHGFTQGNHAINAADVLIGIGMRFDDRVTGRLSEFAPS
                     ARVVHIDIDPAELGKNVSAEVPVVGDARETLKLLLKEVSPTRHDAWLKEIATWRRLPP
                     AAPTDPDGPLAPQTVISRLHALTKGRAIVTTDVGQHQMWAAQLYGCDRPGQFISSGGL
                     GTMGYGVPSALGAALGCPDEDVWAIVGDGGVQMTLFEFATIVQERARVKVAIVNNGYL
                     GMVRQWQELFHNRNYSETPISSPDFVLLAQAYGIPARRVSHPGEVDEAIRWAQAIDGP
                     ALLEFQVNQEANVFPMIPSGGSVSEMIEAVEKIEER"
     gene            1638..2153
                     /gene="ilvN"
                     /locus_tag="VFU72_00025"
     CDS             1638..2153
                     /gene="ilvN"
                     /locus_tag="VFU72_00025"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU5421903.1"
                     /translation="MRRHTLVVLVEDQTGVMNRVMSLFRQRGFNVDSVAVGHSELPGL
                     SRITLAVQGDDRDIEQATKQLYKLLEVIKITDVTGDNLINRELALIKVSASERTRGDI
                     MRLVSEVYRARVVDASPEALVIEVTGPSEKIDSLITLVRPYGIKEVARTGAVAMVRGS
                     GAVAVPQEALA"
     gene            2227..>3194
                     /gene="ilvC"
                     /locus_tag="VFU72_00030"
     CDS             2227..>3194
                     /gene="ilvC"
                     /locus_tag="VFU72_00030"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5421904.1"
                     /translation="MARVYYDNDADLSVLKGKKVAIIGYGSQGHAHAQNLRDSGVDVI
                     VGLYEGSKSWAKAEADGLTVATVQNAAHQADVIMLAIPDTTQKQVYEESIAPELAPGK
                     TLMFAHGFNIHFGRINPPDYVDVSMVAPKGPGHIVRDVYQEGVGVPALLAIHQDASGH
                     ALETALAYAKALGATRAGVLVTTFKEETETDLFGEQAVLCGGVRALVQAGFETLVEAG
                     YQPELAYFEVLHELKLIVDLMYQGGLRYMRYSVSDTAEYGDYVSGPRVIDARVKDTMR
                     KVLEDVQSGAFARRWIDENRQGRPWFDAERKRETEHPIEEVGAKLR"
BASE COUNT          552 a         1082 c         1086 g          474 t
ORIGIN      
        1 gtcgacacga tcttcggcta cccaggcggt gtggtcatcc cgctctacga cgtgctgcct
       61 gagttcggcg ttcaccacgt gctggtgcgc cacgagcagg gcgcggccca cgccgccgac
      121 ggctacgctc gcgccaccgg caaggtcggc gtttgcctgg ccacctccgg ccccggcgcg
      181 accaacctgg tgaccggtat cgccaccgcc atgctcgact ccgtcccgat cgtcgccatc
      241 accggtcagg tctcgcaggc ggtcatcggc tccgacgcgt tccaggagat cgacatcacc
      301 ggcatcacgc tcccgatcac caagcacaac ttcatcatcc gccgcgtcga ggacatcggg
      361 ccgacgatcc agaaggcatt ccatctggcc ggctccggcc gcccgggtcc ggtactggtc
      421 gatatcccca aggacatcct gctcggcaag ggcgtgctcg cccccagcaa tgccgtcaac
      481 ctccctggct acaagcccac ggtcgtcccc caccggcgcc aggtgcgccg cgccgccgag
      541 ctgattcagc aggccgagcg cccgctgatc ctggccggcc acggcatcct gatctccggc
      601 gctagcgacg agttggtgcg cttcgccgag caggcgcaga tcccggtcgg actaacactg
      661 ctcgggttga gcggcttccc tgcctcccac ccgctgtgcc tgggcatggt cggcatgcac
      721 ggcttcaccc aggggaacca cgccatcaac gccgccgacg tgctgatcgg catcggcatg
      781 cgcttcgacg accgcgtcac cgggcgcttg agcgaattcg cacccagcgc ccgcgtggtc
      841 cacatcgaca tcgacccggc cgagctgggt aagaacgtgt cggccgaggt gccggtggtt
      901 ggcgacgccc gcgagacgct caagctcctc ttgaaggaag tctccccgac gcgccacgac
      961 gcctggctga aggagatcgc cacctggcgc cggctcccgc cggccgcgcc caccgacccc
     1021 gacggccccc tggcgcccca gacggtgatc tcgcggctgc atgccctgac gaaggggcgg
     1081 gcgatcgtca cgaccgacgt cggccagcac cagatgtggg cagcgcagct ctatggctgt
     1141 gatcggccgg ggcaattcat cagttcgggc ggtttgggca ccatgggcta cggtgtcccc
     1201 tcagcgctcg gcgcggcgct cgggtgcccg gacgaagatg tttgggccat cgtcggcgac
     1261 ggcggcgtgc agatgacgct cttcgagttc gccaccatcg tacaggagcg ggcgcgggtc
     1321 aaggtggcca tcgtcaacaa cggctacctg ggcatggtgc gccagtggca agagctcttc
     1381 cacaaccgca actactccga gacgcccatc agcagccccg acttcgtgtt gctggcccaa
     1441 gcctacggca tcccggcgcg gcgcgtcagc caccccggcg aggtcgacga ggcgattcgc
     1501 tgggcgcagg ccatcgacgg cccggcgctg ctcgagttcc aggtcaacca ggaggccaac
     1561 gtcttcccga tgatcccctc cggtgggtcc gtgagcgaga tgatcgaagc ggttgaaaag
     1621 attgaggagc ggtaggggtg cgcagacaca cactggtcgt gctggtcgaa gaccagaccg
     1681 gcgtgatgaa ccgggtgatg agtctcttcc gccagcgcgg cttcaacgtc gatagcgtgg
     1741 ccgtcggcca tagcgagctg ccgggcctct cgcgcatcac cctggccgta cagggcgacg
     1801 accgcgacat tgagcaggcg accaagcagc tttacaagct gctggaggtg atcaagatca
     1861 ccgacgtcac cggcgacaat ctgatcaacc gcgagctggc gctgatcaag gtcagcgcct
     1921 cggagcggac gcggggtgat atcatgcgtc tggtttccga agtctaccgg gcgcgagtcg
     1981 tcgacgcgtc tcccgaggcg ctggtgatcg aggtcaccgg gccgagcgag aagattgact
     2041 cgttgatcac gctggtccgg ccatacggaa tcaaagaggt cgcgcggacc ggagcggtgg
     2101 cgatggtgcg cggcagcggc gcggtggccg tgccgcaaga ggcgctggcc tgaagcgcgc
     2161 tccggcggcc tgataggtga gggagggctc acgcgggggg acgcgcgagc gagaggagta
     2221 gcaaacgtgg ctcgtgtcta ctatgacaac gacgccgatc tgtccgtgct caaaggcaag
     2281 aaggtcgcga tcatcggcta cggcagccag gggcacgccc acgcgcagaa cctgcgcgat
     2341 agcggcgtgg acgtgatcgt cggcctgtac gagggcagca aaagctgggc gaaggccgag
     2401 gccgacggcc tgacggtcgc cacggtgcag aacgcggcgc accaagccga cgtgatcatg
     2461 ctggcgatcc ccgacacgac ccagaagcag gtctacgagg agagcatcgc gccggagctg
     2521 gcgccgggca agacgctcat gttcgcccac ggcttcaaca tccacttcgg gcgcatcaac
     2581 ccgccggact acgtcgatgt ctcgatggtc gcgcccaagg gccccggcca catcgtgcgc
     2641 gacgtctatc aggaaggtgt cggcgtgccg gcgctgctgg cgatccacca ggacgccagc
     2701 ggtcacgcgc tggagaccgc gctggcctac gccaaggcgc tcggcgccac ccgcgccggc
     2761 gtgctggtga ccacgttcaa ggaagagacc gagaccgatc tcttcggcga gcaggcggtc
     2821 ctctgcggcg gcgtccgcgc gctggtgcag gccggcttcg agacgctggt cgaggcgggc
     2881 taccaaccgg agctggccta cttcgaggtg ctgcacgagc tgaagctgat cgtcgacctg
     2941 atgtaccagg gcggcctgcg ctacatgcgc tactcggtca gcgacaccgc cgagtacggc
     3001 gactacgtgt cgggcccgcg ggtgatcgac gcgcgcgtca aggacacgat gcgcaaggtg
     3061 ctcgaggacg tgcagagcgg cgcgttcgcg cggcgctgga tcgacgagaa ccgccagggc
     3121 aggccgtggt tcgacgccga gcgcaagcgc gagaccgagc acccgatcga ggaggtcggc
     3181 gcgaagctgc gcga
//
LOCUS       DASUBI010000003         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1117258, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000003 DASUBI010000000
VERSION     DASUBI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117258"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFU72_00035"
     CDS             complement(<1..474)
                     /locus_tag="VFU72_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421905.1"
                     /translation="MDTTSAAREYERSDERSTRLRASLRLIFDDRLADQLAALSGDSK
                     DALRRMARLALRQYRLRQLRSLVHVQQDRDCSRPLAPRRGRGARGAPVLALYLGLDVD
                     LSALADLLQMAPEQLAHELAAARRAADPALPRGGSGEDVLTRAALAADPLWLNRQL"
     gene            614..1021
                     /locus_tag="VFU72_00040"
     CDS             614..1021
                     /locus_tag="VFU72_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5421906.1"
                     /translation="MGERYERRTISSGTVWEERVGYARAVRAGDFVFVSGTTATDDAG
                     DVVGIGDPAAQVRFILEKISAALVAAGATLADVVRYRVYLINADHWEAIAPALRETFG
                     AIRPANTLFEVSRLVGADYLVEIEADAIVGSAG"
     gene            complement(1221..2009)
                     /locus_tag="VFU72_00045"
     CDS             complement(1221..2009)
                     /locus_tag="VFU72_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421907.1"
                     /translation="METVKYGLPDDFWVYAEFSIDSALGGRNVDWLVARAASQSEQLS
                     TLIMTEVKRVSSPLKGLINSPWEKLSDLGEWETIFPRNERDTNYYWQAVNTANALADW
                     LWNYQRLYREGSDLSPDEFRVWPDLLILSQNELIHQLPQRPPNGYGGIYYDPDRWVTH
                     VVRWNPRVGIPLYADDLDRLAGALGLRPIESPTVAPAPVKPPAPHVVPQDGPGGLHSG
                     LRGLEEYLHGLEVRISTLEEELHRLKAVQADVEMPLTPHPVEGL"
     gene            2301..3167
                     /locus_tag="VFU72_00050"
     CDS             2301..3167
                     /locus_tag="VFU72_00050"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5421908.1"
                     /translation="MPLDLPDALVDTAWLAERLDDPRLRVLECTVFLGRPQLESGRAA
                     WEEGHIPGSGFADLIHDLSDPANTRYAFPFPPAEQFAAAMGRLGVGDDSTVVLYDRAG
                     NMWAARLWWMLRAYGFDRAAVLDGGWQAWTAEGRPVSTGPSSYPPARFTPRPRPQLIA
                     DKDEVLAAIDDEQRVLVHALSPEHFAGRASSSSGRTGHIPGSVNVPAFGETGIVDPAT
                     GRYLPLAELRRRVEAADALGGERVITYCGAGIAASSAALALHRLGVRNIAVYDGSLSE
                     WTADPDLPLETE"
     gene            3219..4109
                     /locus_tag="VFU72_00055"
     CDS             3219..4109
                     /locus_tag="VFU72_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEU5421909.1"
                     /translation="MGVADSGLRVVLLSDAAGEIVPVADETLRSAGHRLVGVLTSPGP
                     RKRRGTSRYLDLVAAARPGIDVLVSNHPSRWAAMLAPLRPDVVLSTGFLWVIPDDLLA
                     LPRLGAINTHGGLLPHGRGPNPVGWAFRRDEGVIGWTAHRLTPQLDAGPILAQAAISY
                     GDDDFFADLFQPWTALIPGLWLQALERLSRGEPGEPQVEADAYYAGSFEPEWRFIDWS
                     RPAREIHNQVRSWLGVRGVPEGAIGEIEGVPALITRTRLVDESTTAPPGTVLNRGETL
                     LVQCADRALEIVRWQPVASG"
BASE COUNT          657 a         1495 c         1551 g          702 t
ORIGIN      
        1 gagctggcgg ttgagccaga gcgggtcggc ggcgagcgcg gcgcgggtga ggacatcctc
       61 gccggagccg ccgcgcggca gggcgggatc ggcggcgcgg cgggcggcgg ccaattcgtg
      121 cgccaactgc tcgggtgcca tctgcaagag atcggccagc gccgaaagat cgacatccag
      181 tcccaggtac agcgcgagca ccggcgcacc gcgcgccccg cgaccgcggc gcggcgccag
      241 cggccgcgaa caatcgcggt cctgttgcac gtgcacgagg ctgcgcagtt gccgcaggcg
      301 gtactggcgc agcgcgagcc gggccatgcg ccggagcgcg tccttggagt cgccggacag
      361 cgcggccaat tgatcggcca gccggtcatc aaagatcagc cgcaggctgg cgcgcagccg
      421 cgtgctccgc tcgtcggagc gctcgtactc gcgtgccgcg ctggtcgtgt ccatcgcccc
      481 agcctcgccc ggttgtcgtc gtcgagcgtt tggtgtgccg gccgcatcct accgcgagcc
      541 ggagccgggc gggaagtggt tgagcagcgc cgccggctga cctaccatgc ctcgacaggg
      601 aaaggggacg accatgggtg agcggtacga gcggcgcacg atctccagcg gcacggtctg
      661 ggaggagcgg gtcggctacg cgcgggcggt gcgcgcgggc gacttcgtct tcgtctccgg
      721 caccacagct accgacgacg cgggcgacgt ggtcggcatc ggcgaccccg ccgcgcaggt
      781 ccgcttcatt ctggagaaga tcagcgccgc gctggtggcg gcgggcgcca cgctggccga
      841 cgtggtgcgc tatcgggtct acctgatcaa cgccgaccac tgggaggcga tcgcgccggc
      901 cctgcgtgag accttcgggg caatccgccc ggccaacacg ctcttcgagg tcagccgcct
      961 ggtcggcgct gactacctgg tcgagatcga agccgacgcg atcgtgggga gtgcggggtg
     1021 agggctggcc gtcgaatacg tcgggtgccg tgtgaggaca gatccttcca cttcggttgg
     1081 gatgacaggg ctaatgcggg gctgacgaca acgatgtgaa gtactcgtac gcccccatcc
     1141 cccattcatg tcagcctgag cgtgtcgaag gatccgtgct ccacgcggca acgcgtctcg
     1201 tcggaggccc cgagcgaatc ctagagccct tcgaccgggt gcggggtgag tggcatctca
     1261 acatcggcct ggacggcctt gagccggtgc agctcctcct ccagcgtgct gatgcgcacc
     1321 tccagcccgt gcaggtactc ttccagcccc cgaaggccgc tgtgcagccc gcccggcccg
     1381 tcttggggta cgacgtgcgg cgccggcggc ttgaccggcg ccggtgccac ggtgggcgac
     1441 tcgatcgggc gcagcccgag cgctcccgcc agccggtcga gatcgtcggc gtagagcggg
     1501 atgccgacgc gcggattcca gcgcaccacg tgcgtcaccc agcggtcggg gtcgtagtag
     1561 atgccgccgt agccgttggg cgggcgctgc gggagttggt ggatcagctc gttctgactg
     1621 aggatcagca ggtcgggcca aacacggaac tcgtcgggcg agagatccga accttcgcgg
     1681 tagaggcgct ggtagttcca gagccagtcg gccagcgcgt tggcggtgtt gaccgcctgc
     1741 cagtagtagt tcgtatcgcg ctcgttgcgc gggaagatcg tctcccactc gcccaggtcg
     1801 ctcagcttct cccaggggct gttgatcagc cccttgagcg ggctggagac acgcttgact
     1861 tcggtcatga tcagggtcga gagctgctcc gactggctgg cggcgcgggc gaccagccag
     1921 tcgacgttgc ggccgcccag tgcgctgtcg atgctgaact cggcgtagac ccagaagtca
     1981 tccggcaagc catatttgac tgtttccaaa acctcagtat cgatcttatt ttcgtcgccg
     2041 atgaagatgt gtgccactga cggataaccc tttctccacg ggacacgcaa agcggatcac
     2101 gactttaggt gggtcaacaa atcagttgcc taaagacggt ggatttggcg atgttaatgt
     2161 gcgcgtcgcg ctggtcgtac gccgcgccga gcagcgccgc cgagtccatt atagcccagc
     2221 atagcccagc cattgcgttc ctacgcgccg ggttcaatac ttcgtgggtt gatagattca
     2281 gcgatcaccg aggaggatgc atgccgctcg accttcccga cgcactcgtc gacaccgcct
     2341 ggctggcgga gcggctggac gatccccggc tgcgcgtgct cgaatgcacg gtcttcctgg
     2401 gccgcccaca actggagagc gggcgtgccg cctgggagga agggcacatc cccggcagcg
     2461 gcttcgccga cctgatccac gacctctcgg accccgccaa tacgcgctac gccttcccct
     2521 tcccgcccgc cgagcagttc gctgccgcga tgggccggct cggcgtcggc gacgacagca
     2581 ccgtcgtcct ctacgaccgc gccggcaaca tgtgggccgc gcgcctgtgg tggatgctgc
     2641 gcgcctacgg cttcgaccgc gctgccgtgc tcgatggcgg ctggcaggcc tggacggccg
     2701 aaggacgtcc agtctcgacc gggccgtcga gctatccccc ggcgcgcttc accccgcgac
     2761 cgcgcccgca gcttatcgcc gacaaggacg aggtgctggc cgccatcgac gacgagcagc
     2821 gcgtgctggt ccacgcgctc tcgccggagc acttcgccgg gcgggcgtcg agctcctcgg
     2881 gccgcaccgg ccacatccca ggcagcgtca acgtgccggc cttcggcgag acagggatcg
     2941 tcgacccggc cactgggcgt tacctgccgc tggccgagct gcgccgccgc gtagaggccg
     3001 ccgacgcgct cgggggcgag cgggtgatca cctactgcgg cgcgggcatc gccgccagca
     3061 gcgccgcgct ggcgctgcac cggctcggcg tgaggaacat cgccgtctac gacggctcgc
     3121 tgtccgagtg gaccgccgat cccgatctgc cgctcgaaac cgagtagtgg cgttttggcc
     3181 gcgctgaggg cgaactcgct ccatcgaaag gacatgtgat gggcgttgcc gattcgggcc
     3241 tgcgtgtcgt gctcctctcg gacgctgcag gggagattgt accggtcgcg gacgagacgc
     3301 tgcgttcggc cgggcaccgg ctggtcggcg tgctcacctc gcccggtccg cgcaagcggc
     3361 gcggaacctc gcgctacctg gatctcgtcg cggccgcgcg gccggggatc gacgtgctcg
     3421 tctccaatca tcccagccgc tgggcggcca tgctcgcccc gctgcgtccc gacgtggtgt
     3481 tgagcaccgg tttcttgtgg gtcatccccg acgacctgct ggcgctgccg cgcctgggcg
     3541 cgatcaacac ccacggcggg ttgctgccgc acggccgggg gccgaacccg gtgggctggg
     3601 ccttccgccg cgacgagggt gtcatcggct ggacggctca ccggctcacg ccgcagctcg
     3661 acgccgggcc gatcctggcg caggcggcga tttcctacgg cgacgacgac ttcttcgccg
     3721 atctctttca accctggacc gcgctgatcc cgggcctctg gctgcaggcg ctggagcgtc
     3781 tcagccgggg cgagccggga gagccgcagg tggaagcgga cgcctactac gcgggcagct
     3841 tcgagccgga gtggcgcttc atcgactggt cgcgaccggc gcgagagatc cacaaccagg
     3901 tgcgcagttg gctcggcgtg cggggcgtcc cggagggcgc catcggcgag atcgagggcg
     3961 tgccggcgct gatcacccgc acccggctgg tcgatgagtc gacgacggca ccgcccggca
     4021 ccgtgctgaa ccgcggtgaa acgctgctgg tccagtgcgc cgaccgcgcg ctggagatcg
     4081 tccgctggca gcccgtagcg agcggctagg atgccgcaat aggaattggg tggctgtcgg
     4141 aaacggtcgg aattctctct ggaacgggcg agcgcgggag cgacccgttt gacgccgagg
     4201 agcatggatg ccgcgtcagg acggatcctt cgacgagctc aggctgacat gaatcgggga
     4261 tgggagcgta cgagtacgtc acgtcgcctt cgcccgcccc tgctctaccc cgtcatcctg
     4321 ggcgcggtcg aaggatccgt gctcacccgg cacccaagac gtccgtcaga cggcgcctag
     4381 acctgcgcgg accacacgcc cagcc
//
LOCUS       DASUBI010000004         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1035618, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000004 DASUBI010000000
VERSION     DASUBI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035618"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..139
                     /locus_tag="VFU72_00060"
     tRNA            67..139
                     /locus_tag="VFU72_00060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Thr,seq:cgt)
     gene            complement(433..1581)
                     /locus_tag="VFU72_00065"
     CDS             complement(433..1581)
                     /locus_tag="VFU72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5421910.1"
                     /translation="MATGGQKKRGQNEGSIFRRKNGKWAGAVTIPGSGGKRKWFSGDT
                     QRAVREQVTAARVALDRGESLTMGKLTVGQFLERWLRDVVQPNKAPKTHRTYAGLVRR
                     NIAPGIGGIQLDRLRPQDVQRLLTDLAGGDLAGKAASLQRVREVLRNALNQALRWELV
                     ARNAAALVEPPKYERPSLDPFTPAEARAFLAAAAKDRTSGIYLVAMALGLRQGEVLGL
                     RWEAVDLAAGTLRVEAQVQVVEGKLTLREVKTKRSRRELPIPDIVRRALVAEAARQHG
                     AMLKAGERWQGNAAGLVFTTTVGTPISPRNLMRSYYRILAASGLRLQRFHDLRHFCGS
                     TLAARRVPERQVMEILGHADVRTTQNYYIHVRTEEQRRAVKEMDELLG"
     gene            complement(1904..2125)
                     /locus_tag="VFU72_00070"
     CDS             complement(1904..2125)
                     /locus_tag="VFU72_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5421911.1"
                     /translation="MATEKRAESRTVSVRYAAAQLGIGGSTLYRLIRDGRCPLPVLHL
                     GERVVIPREGLERALREGTRTPAPAPAER"
     gene            complement(2245..>2448)
                     /locus_tag="VFU72_00075"
     CDS             complement(2245..>2448)
                     /locus_tag="VFU72_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5421912.1"
                     /translation="PKEAARALGLSQSYVYELLASETLPSITIGRTRRIPRRALEAFV
                     EARAAEQGGYAPHPIAALRDERA"
BASE COUNT          313 a          924 c          817 g          394 t
ORIGIN      
        1 cacaacaacg agggcagtgg catccagtct ggatgctctt gacccaatgc ggatgccaaa
       61 acgcaggccg acctagctca gttggtagag cagctgtttc gtaaacagca ggtcgcgggt
      121 tcgagtcccg cggtcggctc cgctcagaag cccagtcctg ccgccgaaat ccgacccatg
      181 ccgcgggtcg gatttcgcgt tgtgtcggcc ccgtgcagca acccgatcag caaccgggcg
      241 cgatctgggc tcttccaccc ggttcggccc catgaagaac aggtcatcgc aggggtcgtc
      301 ctcccaccat cgccagggcg gatcgggccg cttccagccg cagtcatgcc agcggcgttg
      361 ctgccagcag cgctgccggg ggaaaccccc gatcgtgacc gtgcgtcctg cgaggtcgcg
      421 catcgtggcc gcctacccga gcagctcgtc catctccttg acggccctgc gctgctcctc
      481 ggtgcggacg tggatgtagt agttctgcgt ggtccgcacg tccgcgtgcc ccaggatctc
      541 catcacctgc cgctcgggca cgcgcctggc ggcgagcgtc gagccgcaga agtgccggag
      601 gtcgtggaag cgctggagcc tgagccccga cgccgccagg atccggtagt agctgcgcat
      661 caggttgcgc ggcgagatcg gcgtccccac ggtcgtggtg aagaccagcc ccgccgcgtt
      721 cccttgccac cgctccccgg ccttcagcat cgcgccgtgc tgccgcgccg cctcggcgac
      781 cagcgcccgc cgcacgatgt cggggatcgg cagctcgcgc cggctgcgct tcgtcttgac
      841 ctcgcgcagc gtcagcttcc cctcgaccac ctgcacctgc gcctcgacgc ggagcgtccc
      901 ggccgccagg tcgacggcct cccagcgcag gcccaggacc tcgccctgcc tgagccccag
      961 cgccatcgcc acgaggtaga tgccgctggt ccggtccttc gccgccgccg ccaggaaggc
     1021 ccgcgcctcc gccggcgtga acgggtcgag cgacggccgc tcgtacttcg gcggctcgac
     1081 cagcgccgcc gcgttgcgcg ccaccagctc ccaccggagc gcctggttca gcgcgttgcg
     1141 cagcacctcg cgcacccgct ggaggctcgc cgccttgccg gcgaggtcgc cgcccgcgag
     1201 gtcggtgagc agccgctgca cgtcctgggg cctgagccgg tcgagctgga tcccgccgat
     1261 gcccggcgcg atgttgcgcc gcaccaggcc ggcgtaggtg cgatgggtct tcggtgcctt
     1321 gttcggctgc accacgtcgc gcagccaccg ctccagaaac tggccaaccg tcagcttccc
     1381 catcgtcagg gactcgcccc ggtccagcgc gaccctggcc gccgtgacct gctcgcgcac
     1441 cgcgcgctgg gtgtcgccgc tgaaccactt gcgcttgccg cccgagccgg ggatcgtcac
     1501 cgccccagcc catttcccgt tcttgcgcct gaagatcgat ccttcgttct ggccgcgctt
     1561 cttctgtccg ccggtcgcca actgccacct ccctccgatg cgacccgcga cgggaggccg
     1621 cagtatcgca catccttgcg ccgtggcggg cgccatcgcc acactccatc ggcgagcgca
     1681 ggcgaccacg gccgtcgtgc ctcatgcgtt cacgttcccg cccgactcgt tccagcgccg
     1741 cttggcgagc cagttgctgt ctggtggagg gcttgacccg cggatcgtcg ggccattgcc
     1801 gctggcgcgt ccgatcaatg cccattccgc ggcgcatgcg ctgctgaaca agcccatcga
     1861 ccgaggtcag gcgcgcttcc ttgtacttgc gtagccctgc cgtctagcgc tcggcgggcg
     1921 caggggcggg ggtgcgggtg ccctccctga gcgcccgctc cagcccctcg cgcgggatga
     1981 ctacccgctc gccgaggtgg aggaccggca gggggcacct gccgtcgcga atgaggcgat
     2041 acagcgtcga gccgccgatc ccgagctggg cggcggcgta gcgcacgctg acggtccgcg
     2101 actcggcccg cttctcagtt gccatggctc atccttcgtc gtccgggggc tcgccggcgc
     2161 ggcccgctcc cggagccccg cgattcagcg gtcgtactcc gcgcgcgtgg tgaggcatca
     2221 tcggtgtttg ccgcgccagc aagctcatgc gcgctcatcg cggagggcgg cgatcggatg
     2281 cggcgcgtag ccgccctgct cggcggcgcg ggcctcgacg aaggcctcca gcgcgcggcg
     2341 cgggatgcgc cgcgtgcggc cgatagtgat cgagggcagt gtctcgctgg ccaggagctc
     2401 gtagacgtag gactggctca ggccgagtgc ccgcgcggct tccttcgg
//
LOCUS       DASUBI010000005         7225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_640496, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000005 DASUBI010000000
VERSION     DASUBI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640496"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1987)
                     /locus_tag="VFU72_00080"
     CDS             complement(11..1987)
                     /locus_tag="VFU72_00080"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU5421913.1"
                     /translation="MPVVAIDLTRREPYAGGQAFGDTGPYERLDGTITFAVDPEHLAN
                     AAITDLKLAPRDAQGRVRFTSDLCLLLPVDQARGNRRLFIELPNRGRKLLPRQLNRAR
                     PEPPTPAIPPGDGFLFRQGYAVGWIGWQWDVYRGDTLMGLEAPEALIDGQRVRGQTVV
                     DIRPNARQRTRLLANRVHKPYPAADLTEAGARLLVRDWEDGENREVPREQWRFARETP
                     EGVVPDAEHIYLEPGFEPGKIYTVLYTAEGAPVVGTGLLAFREIASFLRYDPRSPLAG
                     NVDYAYGFGISQTGRMIRHYIYLGLNQDEAGRVAYDGLLPHVAGARRGEFNHRFGQPS
                     VQTEPSFGHLFPYADAELPDPYGGDSDGLLKRLRAQGQVPKIFYTNSAAEYWRGDAAL
                     LHIDPTGTRDLPDEPESRKYLFASTQHGPGTLPFNRSFPDEGDIGRHDFNVVDYTPLL
                     RAALVNLDRWVSQGVEPPPSKHPRLDDGTAVPAAQALAGVDGIPGIHLPDPARLPTIR
                     KLDLGHQANQGIGHFPAEKGPVYPNYVSAVDADGNETGGIRPADVSVPLGTFTGWNPR
                     APETGAPEQILKMQGITHFFRPTRAERLALDDPRPSLEERYPSKEAYLARVRQAAVAL
                     VAERYALEEDIERIVADAAERWDTAIQGQAIELE"
     gene            2262..3389
                     /locus_tag="VFU72_00085"
     CDS             2262..3389
                     /locus_tag="VFU72_00085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5421914.1"
                     /translation="MRIPAAQIYFPDEDRATILARIGECLATGQLTLGKYGRQLEEEF
                     ARYLGVKHAVAVNSGTSAIEIPLRALGVAGREVLVPANTFFATGAAVIAAGGRPRFVD
                     CDPATLAVDPADLRRAIGPETAGLVHVHIGGLVSPAMPEIRRVCDEAGIWLFEDAAHA
                     HGSAYDGKMAGTFGVAGSFSFYPTKVMTAAEGGMIVTDDDHLAEEARIYRDQGKASFL
                     SNFHTHLGSNWRLSEPHAIIALSQLGRLDEFIAHRQQIARVYNRRLDALPVTPLEVPE
                     GGRCNFYKYVVYLPEGVDRAALKKILREEFEVSLSGEVYDTPMHRQPVFADLVDRPLP
                     GSEAICAGHICLPISAVMTEEQAEYVVDSLARALDRVPALA"
     gene            3417..4346
                     /locus_tag="VFU72_00090"
     CDS             3417..4346
                     /locus_tag="VFU72_00090"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5421915.1"
                     /translation="MRKLIAVTGGSGFIGSHVVDALLGAGYGVRVLDTRAPHREDVEW
                     AAVDLLSDDVADALAETEAVFHLAAAADVNDVYAAPVESVALNSLATVRLLEAARRAE
                     AGRVILASTVWVYAASRQPVVDETTCFEPESDRHLYVSTKIAAELFCRDYHTLYQRPY
                     TILRYGIPYGPRMRDNLVIAAFIRRALRGESIRIDGDGRQERFFVYVEDLARAHVLAL
                     DPRAEGRTYNLDGSRPVSIREIAETVKELVGDVTVEYGPARPGDLAARVVRCDRAREE
                     LGWEPSVSFGEGLGRTLAWYRERLRAEQAVGVG"
     gene            4353..5555
                     /locus_tag="VFU72_00095"
     CDS             4353..5555
                     /locus_tag="VFU72_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5421916.1"
                     /translation="MSASAMQPPKRAGAPLVVAAAALDGGFALFLIAVQAFFPSVLDA
                     GAGAPGYALGIYGATRLLLQAPGGVLARRVGTGRQIALGMGGGALALAALSLAHGALQ
                     AYLLTFVYGLMTALVWPPILLRAGDLFAEHRGELIGYVSLSSAVATVGGVAAGSFLID
                     YIPIRLVLGLAALWLALGALVAGALLAGQRSATPRRGHLAEGADWRGGAAKLFAALLC
                     QGLGLAVLTPIMRQYAIEQLHLRLHDLVVLGLPAAACAALTFLAAGRLTDRLGRAPVV
                     AGGAAIAALGTLLVSQAGGGRYFLLAAIPLGIGYAASAPALSAWVTDLGDRGGGTLGA
                     ALTVQGLALAVGPVLGGLIVSVSRPAVTMQASALFWLLAAVFALLPPLALSRQPHRIE
                     HPSSESSS"
     gene            5573..6337
                     /locus_tag="VFU72_00100"
     CDS             5573..6337
                     /locus_tag="VFU72_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5421917.1"
                     /translation="MPRRIAVVPAYNEEPTVAPVLDRLYPLVDELVVVDDGSTDRTRA
                     EIERWLPGHAHARLLCHARNQGMSEAYYLAFSDLRARLAAGELDPADLIFTVDADGQH
                     ELAVLDELQRIAESERLDALLVRRDLSTYPADKRLGNWLISRWASLWAGVRLLDVESG
                     YRIFRLGALADALNYYTGYQYSETVEVAVVLGRLGYRVRNDVMVPVPVFRSRTRLKDA
                     AIDLAMIPAAALRVARRRPRRTPAVPAAQPPGRQGT"
     gene            complement(6310..7041)
                     /locus_tag="VFU72_00105"
     CDS             complement(6310..7041)
                     /locus_tag="VFU72_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5421918.1"
                     /translation="MRAALALGLVLGSLLTTGWVGASTARANWFGATVDHGPRTGNEV
                     AITFDDGPNAVYSLKIRDILDRYGVKATFFTVGKALDARPDISRALLADGMLLGDHSY
                     HHDSTSWLDPLYPEIPETQASFQQQLGVCPAFFRPPHGTHTPFMAHVAAERGMTMITW
                     DVSAGDWATTDGQLVAQRVLAQVRPGSIILLHDSIDGNVDADRSVMLTALPLILDGLK
                     AKSLQPVRLDQLLSKPGYVPCRPGG"
BASE COUNT          962 a         2471 c         2679 g         1113 t
ORIGIN      
        1 cgattcgcgg ctactcgagc tcgatcgcct gcccctggat ggccgtatcc cagcgctcgg
       61 cggcgtcggc gactatgcgc tcgatgtcct cctccagcgc gtagcgctcg gccaccagcg
      121 cgacggcagc ctggcgcacc cgagcgaggt acgcctcctt cgagggatag cgctcctcca
      181 gggaggggcg cgggtcatcg agcgccaggc gctcggcgcg cgttgggcgg aagaagtggg
      241 tgatcccttg catcttgagg atttgctcgg gcgcgccggt ctcgggggcg cgcggattcc
      301 agccggtgaa ggtgccgagc ggtacgctga cgtccgccgg gcggatgccg ccggtctcgt
      361 tgccgtcggc gtcgaccgcc gagacgtagt tggggtagac cggccccttc tccgccggga
      421 agtgcccgat gccctggttc gcctggtgcc ccaggtcgag cttgcggatc gtgggcagcc
      481 gcgcggggtc ggggaggtgg atgccgggga tgccgtcgac cccggccagc gcctgggccg
      541 cgggcaccgc cgtgccgtcg tccagccggg gatgtttgct cggcggcggc tcgacgccct
      601 ggctgaccca gcggtcgaga ttgaccagcg cggcgcgcag cagtggggtg tagtcgacca
      661 cgttgaagtc gtgccggccg atgtcgccct cgtcgggaaa ggagcggttg aacgggagcg
      721 tgccggggcc gtgctgggtg ctggcgaaga ggtatttgcg actctccggt tcgtcgggta
      781 ggtcgcgtgt gccagtcggg tcgatgtgca ggagcgcggc gtcgccacgc cagtactcgg
      841 cggcgctgtt ggtatagaag atcttgggca cctgaccctg ggcgcgcagc cgcttgagca
      901 gcccgtcgct gtcgccgccg taggggtcgg gcagctcggc gtcggcgtag gggaagaggt
      961 ggccgaagct cggctcggtc tgcaccgagg gctggccgaa acggtggttg aactcgccgc
     1021 gccgcgcgcc ggccacatgc ggcagcaggc cgtcgtaggc cacgcgcccg gcctcgtcct
     1081 ggttgaggcc gaggtagatg tagtggcgga tcatgcggcc ggtctgggag atgccgaagc
     1141 cataggcgta gtcgacgttg ccggccagcg ggctgcgcgg gtcgtagcgc aggaagctgg
     1201 cgatttcgcg gaaggccagc agtccagtgc cgacgaccgg tgcgccctcg gcggtgtaga
     1261 gcaccgtgta gatcttgccc ggctcgaagc ccggctccag gtagatgtgc tcagcgtccg
     1321 gcacgacccc ttccggcgtc tcgcgggcga agcgccactg ctcgcgcggc acttcacggt
     1381 tctcgccgtc ttcccagtcg cgcaccagca agcgcgcccc cgcctcggtc aggtcggcgg
     1441 ccgggtaggg cttgtggacg cggttggcca gcaggcgcgt gcgttggcgg gcgttggggc
     1501 ggatgtcgac cacggtctgg ccacgcaccc gctgaccgtc gatcaacgcc tcgggcgcct
     1561 ccaggcccat cagcgtgtcg ccgcggtaga cgtcccactg ccagccgatc cagccgacgg
     1621 cgtagccctg gcggaagagg aagccgtcgc cgggcgggat ggccggtgtg ggcggctcgg
     1681 ggcgggcgcg gttgagctgg cgcggcagga gcttgcgccc gcggttgggc agctcgatga
     1741 acaggcggcg gttgccgcgc gcttgatcga ccggcagcag caggcaaaga tccgaggtga
     1801 agcgcacccg cccctgcgcg tcgcgcggag ccagcttcag atcagtgatc gccgcgttgg
     1861 ccaggtgctc ggggtcgacg gcgaaggtga tcgtaccgtc taaccgctcg taggggccgg
     1921 tgtcaccgaa tgcctggccg cccgcgtacg gctcgcggcg cgtcaggtcg atcgctacca
     1981 caggcatcgc atcctctttc cggtcatcac gcatggtcga gctacggggg catgatacgg
     2041 catcgggtgg gggggtgtcg ctcacagggc agcgctcatc gtggacccgg agaaccggtg
     2101 ccgcgcgacg cgcggcacga aagccccggg tagattgcca acctccccat acatacgcgc
     2161 tttggcgtat ttggtcgcgc ccggtgtgct cctacactcc cggggtgaag cggacagggc
     2221 attgggtcac gaaccgcgac cacgcgagag gaacgtctgc catgcgcatc ccagcggcac
     2281 agatctattt cccggacgag gaccgcgcca ccatcctggc gcgcatcggc gagtgcctgg
     2341 cgaccggcca gctcacgctc ggcaaatatg gccgccagct cgaagaggag ttcgcgcgct
     2401 acctcggcgt caagcacgct gtcgcggtca actcgggcac cagcgccatc gagatcccgc
     2461 tgcgcgcgct gggcgtggcc gggcgcgagg tgctggtgcc ggccaatacc ttcttcgcca
     2521 ccggcgcggc ggtgatcgcg gccggtggcc gcccgcgctt cgtcgactgc gacccggcca
     2581 cgctggcggt cgaccccgcc gacctgcggc gggccatcgg tccggagacg gccggcctag
     2641 tgcacgtcca catcggcggg ctggtctcgc cggccatgcc ggagatccgg cgcgtctgcg
     2701 atgaggccgg catctggctc ttcgaggacg cggcccacgc ccacggcagc gcctacgacg
     2761 gcaagatggc cggtaccttc ggcgtcgccg gctccttctc gttctatccc accaaggtga
     2821 tgaccgccgc cgagggcggc atgatcgtca ccgacgacga ccatctcgcc gaggaggcgc
     2881 gcatctaccg cgaccagggc aaggcgtcct tcctctccaa cttccacacc cacctcggct
     2941 ccaactggcg cctgagcgag ccgcacgcca tcatcgcgct ctcccagctt gggcgcctcg
     3001 acgagttcat cgcgcaccgc cagcagatcg cccgcgtcta caaccgccgg ctcgacgccc
     3061 tgccggtcac gccgctggag gtgcccgagg gcgggcgctg caacttctac aagtacgtcg
     3121 tctatctgcc cgagggggtc gaccgcgcgg cgctcaagaa gattctgcgc gaggagttcg
     3181 aggtgagcct gagcggcgag gtgtacgaca cgccgatgca ccggcagccg gtcttcgccg
     3241 acctggtcga tcgcccgctg ccgggctctg aggcgatctg cgccggccac atctgcctgc
     3301 cgatctcggc agtgatgacg gaagagcagg ccgagtacgt ggtcgactcg ctggcgcggg
     3361 cgctcgaccg cgtgccggcg ctggcgtaga gttgaatgaa ggggaatgat ctcaccatgc
     3421 ggaagctcat cgcggtcacc ggaggctcag ggttcatcgg ctcgcacgtg gtcgatgccc
     3481 tgctcggagc cggctacggc gtgcgcgtgc tcgacacccg cgcgccgcac cgtgaggatg
     3541 tggagtgggc cgccgtcgac ctgctgagtg acgacgtggc cgacgccctt gcggagaccg
     3601 aggccgtctt ccacctggcc gccgcggctg acgtcaacga cgtctacgcc gcgccggtcg
     3661 agagcgtggc gctcaacagt ctggccacgg tccggctgct ggaggccgcg cgccgggccg
     3721 aggccgggcg cgtcatcctg gccagcaccg tctgggtcta cgccgccagt cgccagccgg
     3781 tggtcgacga gacgacctgc ttcgagccgg agagcgaccg gcacctctac gtcagcacca
     3841 agatcgcggc cgagctcttc tgccgcgact accacacgct ctaccagcgg ccgtacacca
     3901 tcctgcgcta cggcatcccc tacggccctc gcatgcgcga caacctggtg atcgcggcct
     3961 tcatccgccg ggcgctgcgc ggcgagtcga tccgcatcga cggcgacggt cgccaggagc
     4021 gcttcttcgt ctacgtggag gatctggcgc gggcgcacgt gctggcgctc gacccacgcg
     4081 cggagggccg cacttacaac ctcgacggca gccgcccggt cagcattcgc gagatcgccg
     4141 agacggtcaa ggagttggtg ggcgacgtga cggtggagta cggaccggcg cgacccggcg
     4201 atctggccgc gcgcgtggtg cgctgcgacc gggcgcgtga ggagctgggc tgggagccgt
     4261 cggtctcgtt cggcgagggg ctgggccgta cgctggcctg gtatcgcgag cgcctgcgcg
     4321 ccgagcaggc cgtcggcgtc ggctaggccg agatgagcgc gagcgcgatg cagccgccga
     4381 agcgcgccgg ggcgcccctg gtcgtggcgg ccgccgcgct cgacggcggc ttcgcgctct
     4441 tcctgatcgc ggtgcaggcc ttcttcccga gcgtgctcga cgccggcgcg ggcgcgccgg
     4501 gctacgcgct gggcatctac ggcgccacgc ggctgctgtt gcaggcgccg ggcggggtgc
     4561 tggcgcggcg cgtcggcacg ggccggcaga tcgcgcttgg catgggcggg ggcgcgctgg
     4621 cgctggcggc gctcagcctg gcgcacggcg cgctccaggc ctatctgctc accttcgtct
     4681 acggccttat gaccgcgctg gtctggccgc cgatcctgct acgggccggc gatctcttcg
     4741 ccgagcatcg cggcgaattg atcggctacg tcagtctcag ctcggcggtc gcgaccgtgg
     4801 gcggggtggc cgccggctcc ttcctgatcg actacatccc gatccggctg gtgctggggc
     4861 tggcggcgct ctggctggcg ctgggggcac tggtcgcggg cgcgctgctc gccggccagc
     4921 gaagcgccac gccgcgtcgc gggcacctgg cggagggcgc ggactggcgc ggcggggcgg
     4981 ccaagctctt cgccgcgctg ctctgccagg ggctcggcct ggcggtgctg acgccgatca
     5041 tgcgccagta cgcgatcgag cagctccacc tgcggctgca cgacctggtc gtgctcggcc
     5101 tgccggcggc cgcctgcgcc gcgctgacct tcctggcggc gggtcgcctg actgaccggc
     5161 tgggccgggc gccggtggtg gcgggcgggg ccgccatcgc ggcgctgggc acgctgctgg
     5221 tctcgcaggc gggcggcggg cgctacttcc tgttggcggc gatcccgttg gggatcggtt
     5281 acgccgccag cgcaccggcg ctgagcgcct gggtgaccga cctgggcgat cgcggcggcg
     5341 gcacgctcgg cgccgcgctg acggtgcagg ggttagcgct ggccgtcggt ccggtgctgg
     5401 gcgggctgat cgtcagcgtc agccggccgg ccgtcaccat gcaggcctcg gcgctcttct
     5461 ggttgttggc ggccgtgttt gccctgctgc cgccgctcgc gctctcgcgt cagcctcacc
     5521 ggatcgagca tccatcttct gaaagcagtt cgtagcgtcg aaggagccgc ccatgccgcg
     5581 acggatcgcc gtggtgcccg cctacaatga ggagccgacg gtggcgccgg tgctcgaccg
     5641 gctctacccg ctggtcgacg agttggtggt ggtcgacgac ggctcgaccg accgcacgcg
     5701 cgccgagatc gagcgctggc tccccggcca cgcgcacgcg cggctcctct gccacgcgcg
     5761 caaccagggg atgtccgagg cctactacct ggccttcagc gacctgcgcg cccggctcgc
     5821 ggccggcgag ctggaccccg ccgacctgat cttcaccgtc gacgccgacg gccagcacga
     5881 gctggcggtg ctcgacgagc tgcagcgcat cgccgagtcc gagcggctcg acgcgctgct
     5941 ggtgcggcgc gacctctcga cctatccggc cgataagcgg ctcggcaact ggctgatcag
     6001 ccgctgggcc agtctctggg cgggcgtccg gctgctggac gtcgagtcgg gctaccgcat
     6061 ctttcggctg ggcgcgctgg ccgacgcgct caactactac accggctacc agtacagcga
     6121 gacggtcgag gtggcggtgg tgctggggcg gctcggctac cgggtgcgca acgacgtcat
     6181 ggtgccggtg ccggtcttcc gctcgcgcac gcggctgaag gacgccgcca tcgacctggc
     6241 gatgatcccg gccgccgcac tgcgcgtggc ccgccgccgg ccacggagaa ccccggctgt
     6301 acccgccgct cagccgccgg ggcggcaggg gacgtagccc ggcttgctca ggagctggtc
     6361 gagccgcacc ggctgcaggc tcttcgcctt gagaccgtcg aggatcagcg gcagggcggt
     6421 gagcatcacc gagcggtcgg cgtcgacgtt gccgtcgatg ctgtcgtgca gcaagatgat
     6481 cgagccgggc cgcacctgcg ccagcacccg ctgggccacg agctggccat cagtggtggc
     6541 ccagtcgcct gccgagacat cccaggtgat catcgtcatg ccgcgctcgg cggcgacgtg
     6601 cgccatgaag ggggtgtggg tgccgtgtgg cgggcggaag aaggcggggc agacgccaag
     6661 ctgctgttgg aagctggcct gcgtctcggg gatctcgggg tagaggggat cgagccagct
     6721 ggtcgagtcg tggtggtagg agtggtcgcc cagcagcatg ccgtcggcca gcagggcacg
     6781 cgagatgtcg gggcgcgcat ccagcgcctt gcccacggtg aagaaagtag ccttcacgcc
     6841 gtagcggtcg aggatgtcgc ggatcttgag cgagtagacg gcgttgggac cgtcgtcgaa
     6901 ggtgatggcg acctcgttcc ccgtgcgcgg accgtggtcg accgtggcgc cgaaccagtt
     6961 ggcccgggcg gtgctggcgc ccacccagcc ggtcgtcagc aggctgccga ggacgaggcc
     7021 gagggcgagg gcggccctca ccccccaacc ccctctccca attctgggcg agggggggac
     7081 tgtagcggcg gctccgtccg gtttcgatcc cgcccaacca ccgctcgccc gttcggaact
     7141 gacgcaaatc accgcattca gcgtgatgaa gatgagtccc gcgatcaacg cgaccgggag
     7201 gctgaggcgc caaagggcga cgagg
//
LOCUS       DASUBI010000006         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_626757, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000006 DASUBI010000000
VERSION     DASUBI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626757"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..370
                     /locus_tag="VFU72_00110"
     tRNA            300..370
                     /locus_tag="VFU72_00110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Cys,seq:gca)
     gene            complement(480..959)
                     /locus_tag="VFU72_00115"
     CDS             complement(480..959)
                     /locus_tag="VFU72_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421919.1"
                     /translation="MRYSRRLVAVLIAGVIVALLSAMPQTANAADRAPFGLILVGQDT
                     LVTDNCPTICTEHTGAGFATQFGAFKSSGFIVATSIRFLSKTQLKATTVETYVFTATT
                     GDTLDVTNYATGIDDLTTGLTSITGHGSSRAGPESSVAPLGAERPAGRCARASWRAL"
     gene            complement(1108..>2789)
                     /locus_tag="VFU72_00120"
     CDS             complement(1108..>2789)
                     /locus_tag="VFU72_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU5421920.1"
                     /translation="YVKLGRLDGIHDLRDESDRTGMRIVIELKRDAQPTRVLNGLFKH
                     TQLQTTFGVNMLALVEHGTQPRVLGLRRVLQLYIEHREEVVRRRTQFDLRRARRRAHL
                     AEGLKIALDHLDEVIRTIRESRTADVARSSLIARFALTEAQANAILEMQLRRLAALER
                     RRIEEEYRELLREIAELEGLLADPAKILAVIRQEIVDLRQRFGDERRTQIQDLSGEIS
                     DEDLIPEVRVLVTITNRGYIKRLPDDTYRTQHRGGRGVTGMALREEDGVQHIVLGNTH
                     DSLLFFTNRGRVFQLKVYEIPDASRTAKGIPVINLVGLQPHETITTMLPVHDFTSADY
                     LFMCTRNGRVKRTSLDQFSSVRSTGMIAIGLDDDDELAWVRMTSGENQIILVTERGQA
                     IRFEESDVRAMGRPAAGVIGVRLEDGDRVIAAEVARPDADLLVVSINGYGKRTAIEEF
                     RLQGRGGYGVTAMKLSERNGPIAAARVVDPDDQIMLITGRGMVIRVQAGQISQIGRQT
                     QGVSIMRVQDGDHVASLTRISTPEEAEAADVAEASAAPSVTTSSNGTGDKH"
BASE COUNT          440 a          918 c          948 g          483 t
ORIGIN      
        1 acgggccgat gctgaaggtc gcggccacgc tgaaggcgaa cgagagcagc cccgccagcc
       61 agagcagctt ccaggagcgg cggtcgaagc ccaccacggt tacgaggatt gagatgatca
      121 gcagcgacca gagcgccgcg gcgatgagtt gataggtcaa cggttcactc ccattgtccg
      181 ggctgagtca cgcgaggagc gtgaggccga acaccagtac gcggaacacc accggcggcg
      241 ctatcacgga tggtcggccg ggggccgtat ggtacacttt cgcctgtaca tcgcaattcg
      301 gcgccgtcgc caagtggcaa ggcaggagtc tgcaaaactc tcaccggcgg ttcgaatccg
      361 cccggcgcct ccccaggaat cacattcagc gcgaggtcaa cctctccgtg gcgacggtcc
      421 agcgatccgt tagactccgc caggtatgga gatcgtaccg accagggtgg cggtcccgct
      481 caaagtgccc gccaggaggc gcgcgcgcaa cgtcccgctg gtcgttccgc tcccagaggc
      541 gccactgaac tttccggtcc cgcccgtgat gacccatgcc cggtgatcga ggtcaggccg
      601 gtggtgagat catcaatccc ggtggcgtag ttcgtcacat ccaacgtatc gccggttgtg
      661 gccgtgaata cgtaggtttc gacggtcgtt gccttgagct gggtcttgga gaggaaccgt
      721 atggaagtgg cgacgatgaa cccgcttgac ttgaaggcgc cgaactgggt cgcgaaccct
      781 gctccagtgt gttcggtaca gatggtcgga cagttgtccg tcaccagcgt atcctgaccg
      841 acgaggatca gtccgaacgg cgctcggtcc gcggcgtttg ccgtttgcgg catggcgctg
      901 agcaaggcaa cgatcacgcc cgcgattaac actgcgacga gccgccgaga atagcgcatg
      961 gtgacctcgc tccctgccct gcccggctac gatcgtgaac ccggaacgtc catcgcacag
     1021 gctcgtgcac acccgcggcg cgtgcaagcg caggctgccg gcatggggcc agaatagcgg
     1081 ataacgatac gcgcgctagt cccagcgtca gtgcttgtcg ccggtgccgt tgctggatgt
     1141 cgtgacgctg ggggcggcgg aggcctcggc gacgtcggcg gcttcggcct cctcgggcgt
     1201 gctgatgcgg gtcagggagg cgacgtggtc gccgtcctgt acgcgcatga tgctgacacc
     1261 ctgcgtctgc cgcccgatct gtgagatctg gcccgcctgc acccggatga ccatgccccg
     1321 accggtgatc agcatgatct ggtcgtcggg atcgaccacc cgcgccgccg cgatcggccc
     1381 gttgcgctcg ctcagcttca tggccgtcac cccatagccg ccgcgaccct gcagccggaa
     1441 ctcctcgatc gcggtgcgct tgccgtagcc gttgatcgag acgaccagca ggtcggcatc
     1501 cggccgcgcc acttcggccg cgatcacccg gtcgccgtcc tccagccgca cgccgatcac
     1561 gccggctgcc gggcggccca tcgcgcgcac atcgctctcc tcgaagcgga tcgcctggcc
     1621 gcgctcggtc accaggatga tctggttctc gccgctggtc atgcgcaccc aggccagctc
     1681 gtcgtcgtca tccagcccga tggcgatcat gccggtggag cgcaccgagg agaactgatc
     1741 caggctggtg cgcttgacgc gcccgttgcg ggtgcacatg aagaggtagt cggcactggt
     1801 gaaatcgtgc accggcagca tggtggtgat cgtctcgtgc ggctgcaggc cgaccaggtt
     1861 gatcactggg atgcccttgg cggtgcggct ggcgtcgggg atctcgtaga ccttgagctg
     1921 aaagacgcgg ccgcggttgg tgaagaagag gaggctgtcg tgggtgttgc ccagcacgat
     1981 gtgctgcacc ccgtcctcct cgcgcagcgc catgccggtc acgccccggc cgccccgatg
     2041 ctgggtgcgg taggtgtcgt ccggcagccg cttgatgtag ccacggttgg tgatggtcac
     2101 cagcacgcgc acctcgggga tcagatcctc gtcgctgatc tcgcccgaga ggtcctggat
     2161 ctgggtgcgg cgctcgtcgc cgaagcgctg gcgcaggtcg acgatctcct gccggatcac
     2221 cgccaggatc ttggccgggt cggccagcag accttccagc tcggcgatct cgcgcagcag
     2281 ctcgcggtac tcctcttcga tcctgcgccg ctccagcgcg gccaggcggc gcagctgcat
     2341 ctcgagaatc gcgttggcct gcgcctcggt cagtgcgaag cgcgcgatca ggctgctccg
     2401 cgcgacatcg gcggtgcgcg actcgcgaat ggtgcggatc acctcatcca gatggtcgag
     2461 cgcgatcttg agcccttcgg ccaggtgggc gcggcggcgg gcgcgccgca ggtcgaactg
     2521 ggtgcggcgg cgcacgacct cctcgcggtg ctcgatgtag agctgcagca cgcggcgcag
     2581 cccgagcacg cgcggctgcg tgccgtgctc caccagcgcc agcatgttga cgccgaaggt
     2641 cgtctgcaat tgcgtgtgct tgaagaggcc attgagcacg cgcgtcggct gggcgtcgcg
     2701 cttgagctcg atcacgatgc gcatgccggt gcggtccgac tcgtcgcgca ggtcgtggat
     2761 gccgtcgagg cggccgagct tgacgtact
//
LOCUS       DASUBI010000007         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_27215, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000007 DASUBI010000000
VERSION     DASUBI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27215"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..3627
                     /locus_tag="VFU72_00125"
     CDS             301..3627
                     /locus_tag="VFU72_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5421921.1"
                     /translation="MGILAVWRIVLKRARADWLPLSAALLVVLFATTLLAAGPIYADA
                     VSVSGLRRTLHDAPPTSANVELSTFAQNSDYGALDQQVTRVASETFAATGGTIMRSAR
                     SESYALPGQAPDAVTNLMVFGYYQQLDQHAKLASGRWPQRADGVVEAIVPDATARLLH
                     LSLGSELTVTNRLDEKVQTRVRVVGTYKIDDTGDPYWWQDPLDLNGIDTGASFTTYGP
                     FVLAQDVFFGGLGRPQSQVNWRIYPDFQKLTVAQLAGLLANVNALPGRLNQGRGPNNN
                     IQVHSDLADILRAAQRSLLVSRSGVLVVTVQLALLAGYALLLTAGLLVDGRRVETGLL
                     YSRGAGAGQIAFLSLLEAALIAIPAALAAPWLAALSLRVMNHIGPLAAISLPLKPEVG
                     RASYLLALVAALGCVVALTIPARQSAQALVQARAARGRQRRQGVAQRAGIDVVLLLAA
                     ALGYWQLRRYGAPITETVQGKLGLDPFLIVAPALGLLAGAVAALRVIPLLARLVDQLA
                     ARRRGLVPALGGWQLARRPLRYTRSALLLTMAIAIGLFALAYSATWGLSQGDQANYQV
                     GADLRVQPDQRTGIAIPALDRDQAYRQLAPVEETMPVARDSFEISRAAGDAQLLALDS
                     VAAPGVVELRPDLASSGSLAELMGRLKVGRPALPSIALPGQPQALALDLRYTLDPLPP
                     GTTLPRGESAPVPWLSLVVQDQHGMPFSVTVGAVKADGTTQHVVVPLAVRTDDGRLLG
                     PGAPLALVDLELSLQASRVIYSGTVEVSGLAVSDQLDGGSWTPVALPSGADSFGWSMQ
                     QYSLAEAATANPASQQPAGGTAVRFTTGRVRGNNSYPLTLKLAAPAIKQTSLPALVSQ
                     SFLDKTASKVGDTLQLDVAGARRDVQLVGALRDFPTADPTKPVVVVDLPTLAILHYET
                     DGNIPEPEEDWLAVRPGQEAAVADALRAAPYFSVSVSDRLDRGETLRSDPVALGIIGA
                     LSLGFVAAALFAAVGFAVSAAVSARERLREFALLRALGLSGGQLSGWLTLESGLLIAI
                     SLVGGTILGFLLAQLILPLLSLNQQATHAVPPATVVVPWLQVAVIEASTLLVLALVVT
                     ALALALRRLGLGSSLRLGEE"
     gene            3631..>4039
                     /locus_tag="VFU72_00130"
     CDS             3631..>4039
                     /locus_tag="VFU72_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421922.1"
                     /translation="MARTLGVRRAYVRTAPAAVLLLLRRLRVELALAATVIGVVLLTS
                     GVFSSFPRLYNRLSDDGLRYAIAHANVFERNIVMYHGARVAPGASDPFSEVDAAGAQF
                     QQSLAPSIRQVIDHRDFVVDAPEYRFPEPPGPPP"
BASE COUNT          501 a         1488 c         1434 g          616 t
ORIGIN      
        1 tgatcctgga gacacggcac cgctcggcgc cgcctccggc cggctggagc ccgccgcgct
       61 gaagcaacga acgcccgcga cggaggctga ggtcgcgcgc tccacgagtc gcgacgttgt
      121 ggaacctggg gataggcacc cgacggggga gattcttcgg cagcggtctc aggttgagcg
      181 ggaaaaccgg tctttcgccg cgcagccgct ccaacggcgc gcacggctcg ctctcccacc
      241 cttcggctaa atttgctacc atctcgctga attgtttccg tctggagcgg aaagcgggcc
      301 gtgggaatcc tggcggtgtg gcgcatcgtg ctcaagcgtg cgcgggcaga ctggctgccc
      361 ctcagcgccg cgctgctggt cgtgctcttc gcgaccaccc tgctggccgc cgggccgatc
      421 tacgccgacg cggtcagcgt cagcgggctg cggcgcacgc tgcacgacgc gccgcccacc
      481 tcggccaacg tcgagctgtc cactttcgcc cagaactcgg actacggcgc gctcgaccag
      541 caggtgacgc gcgtcgccag cgagaccttc gccgccaccg gcggcacgat catgcgctcg
      601 gcccgctccg agtcgtacgc cctgccgggg caggctcccg acgcggtcac caatctcatg
      661 gtcttcggct actaccagca gctcgaccag cacgccaaac tcgctagcgg gcgctggccg
      721 cagcgggccg acggggtcgt cgaggcgatc gtgcccgacg ccaccgcgcg gctgctgcat
      781 ctctcgctcg gcagcgagct gacggtgacc aaccggctgg acgagaaggt gcagacccgg
      841 gtgcgtgtgg tcggcaccta caagatcgac gacaccggcg acccgtactg gtggcaggac
      901 ccgctcgacc tgaacgggat cgacaccggc gcctccttca ccacctatgg ccccttcgtc
      961 ctggcccagg atgtcttctt cggcgggctg ggccgcccgc agtcgcaggt gaactggcgc
     1021 atctaccccg acttccagaa gctgaccgtg gcccagctcg ccgggctgct ggccaatgtc
     1081 aacgcgctcc cggggcggct caaccagggc cgcggcccca acaacaatat ccaggtgcac
     1141 tccgatctgg ccgacatcct gcgcgcggcg cagcgctcgc tgctggtcag ccgcagcggc
     1201 gtgctggtgg tgacggtgca gctggcgctg ctggccggct acgcgctgct gctgacggcc
     1261 gggctgctgg tcgatgggcg gcgcgtggag accggcttgc tctactcgcg cggggcggga
     1321 gcgggccaga tcgccttcct ctcgctgctg gaggccgccc tgatcgctat ccccgccgcg
     1381 ctagcggcgc cctggctggc cgcgctctcg ctgcgggtca tgaaccacat cggcccgctg
     1441 gccgccatct ccctgccgct caagccggag gtcgggcgcg cctcctacct gctggcgctc
     1501 gtcgccgccc tcggctgcgt cgtcgcgctg acgatcccgg cgcggcagtc ggcgcaggcg
     1561 ctggtgcagg cgcgggcggc gcgtgggcgg cagcggcgcc agggcgtggc gcagcgggcc
     1621 ggcatcgacg tggtgctgct gctggccgcc gcgctcggct actggcaact gcggcgctac
     1681 ggcgcgccga tcaccgagac ggtgcagggc aagctcgggc tcgacccctt cctgatcgtg
     1741 gcgccggccc tggggctgct ggccggagcg gtggccgcgc tgcgggtcat cccgctgctg
     1801 gcgcggctgg tcgatcagct cgccgcgcgc cgccggggac tggttccggc gctcggcggc
     1861 tggcagttgg cgcgccgccc gctgcgctac acccgctcgg cgctcctcct gacgatggcg
     1921 atcgcgatcg gcctcttcgc gctggcctac agcgcgacct ggggcctctc gcagggcgac
     1981 caggccaact atcaggtcgg cgctgacctg cgcgtgcagc ccgaccagcg caccggcatc
     2041 gccatcccgg ctctcgaccg cgatcaggcc taccgccagc tggcgccggt cgaggagacc
     2101 atgccggtgg cgcgcgacag tttcgagatc tcgcgcgcgg ccggcgacgc gcagctcctg
     2161 gcgctcgaca gcgtcgccgc gcccggggtg gtcgagctac ggcccgacct ggccagcagc
     2221 ggctcgctgg cggagctgat gggccggctc aaggtggggc ggccggcgct gccgtcgatc
     2281 gccttgccgg ggcagccgca ggcgctggcg ctcgacctgc gctacacgct cgatcccctg
     2341 ccacccggca cgacgctgcc acgtggcgag agcgcgccgg tgccctggct ctcgctggtg
     2401 gtgcaggacc agcatggcat gcccttcagc gtcaccgtcg gcgcggtcaa ggccgacggc
     2461 accactcagc acgtggtggt gccgctggcg gtgcgcaccg acgacggacg cctgctcggc
     2521 cccggcgcgc cgctggcgct ggtcgatctc gagctgagcc tgcaggcctc gcgcgtgatc
     2581 tacagtggga cggtcgaggt cagcggactc gccgtcagcg atcagctcga tgggggaagc
     2641 tggacgccgg tagcgctgcc gtccggcgcg gacagcttcg gctggagcat gcagcagtac
     2701 agcctggccg aggcggccac cgccaatccg gccagccagc agccagccgg tggtacggcg
     2761 gtgcgcttca ccaccggtcg cgtgcgcggc aacaacagct acccgctgac gctgaaactg
     2821 gccgcgccgg cgatcaagca gacgagcctg ccggcgctgg tcagccagtc gttcctggac
     2881 aagaccgcct ccaaggtagg cgacacgctg cagctcgatg tggctggggc acggcgcgat
     2941 gtccagctcg tcggcgcgct gcgcgatttc cccaccgccg atccgaccaa gccggtggtg
     3001 gtggtcgatc tgccgacgct ggccatcttg cactacgaga ccgacggcaa tatccccgag
     3061 ccggaggagg actggctggc ggtgcggccg ggccaggagg cggcggtcgc cgatgccctg
     3121 cgcgccgcgc cctacttcag cgtctcggtc agcgaccggc tggaccgggg tgagacgcta
     3181 cgctccgatc cggtggcgct ggggatcatc ggcgcgctct cgctcggctt cgtggcggcc
     3241 gcgctcttcg cggcggtcgg tttcgcggtc agcgcggcgg tctcggcccg tgagcgcctg
     3301 cgcgagttcg cgctgctgcg ggcgctcggg ctctccggcg gccagctctc cggctggctg
     3361 acgctggaga gcggcctcct gatcgcgatt agcctggtgg gcggcaccat cctcggcttc
     3421 ctgctggccc agttgatcct gccgctcctc tcgctcaacc agcaggcgac gcacgccgtg
     3481 ccgccggcca ccgtggtcgt cccctggctc caggtagcgg tcatcgaggc cagcacgctg
     3541 ctggtgctgg cgctggtggt gacggcgctg gcgctggcgc tgcggcggct cgggctgggc
     3601 agctcgctgc ggctgggaga ggagtagccg gtggctcgta cgctcggcgt ccggcgcgcc
     3661 tacgtccgga ccgctccggc ggcggtcttg ctgctgctgc gccggctgcg ggtcgagctg
     3721 gcgctggccg ccaccgtcat cggcgttgtc ctgctgacca gcggtgtctt cagctccttc
     3781 ccgcgcctct acaaccgcct ctccgacgac ggcctgcgct acgccatcgc ccacgccaac
     3841 gtcttcgagc gcaacatcgt gatgtaccac ggcgcacgcg tcgcgcccgg tgccagcgac
     3901 cccttcagcg aggtcgacgc ggccggcgcg cagttccagc agagcctggc gccctcgatt
     3961 cgtcaggtga tcgaccaccg cgacttcgtg gtcgacgcgc cggagtaccg cttccccgag
     4021 ccgcccggcc cgccgccgg
//
LOCUS       DASUBI010000008         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_517339, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000008 DASUBI010000000
VERSION     DASUBI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517339"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFU72_00135"
     CDS             <1..844
                     /locus_tag="VFU72_00135"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEU5421923.1"
                     /translation="AAPNPARQVEARPGAAWRGAVARGVAAIRAGELEKVVLARAVLL
                     EAERAFAAPAALARLAVAYPSCTVFAFGRAGRTFLGATPERLARLEDGVLRTAALAGS
                     APRGATPEEDARLGQGLLASAKDRGEHAIVVRALTDGLEGICDELTMPGEPALLSVAN
                     IHHLHTPVSGRLGPGKTLLDAVERLHPTPAVGGWPRAAALRFIREQEGLDRGWYAAPL
                     GWLDRHGAGEFAVGLRSALLEGSTATLFAGCGIVGDSDPAEEYAESRLKLRPMLAALT
                     GCAR"
     gene            841..>2265
                     /gene="menD"
                     /locus_tag="VFU72_00140"
     CDS             841..>2265
                     /gene="menD"
                     /locus_tag="VFU72_00140"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEU5421924.1"
                     /translation="MRYEEAAYVYLGAFVEALAQAGVRDVCLAPGSRSTPLALTLARN
                     PAIKLWLQLDERSTAFFALGLAKASGRPVGLLCTSGTAAANFFPAVVEAHYARVPLLV
                     MTADRPPELRDAGAAQTIDQVRLYGAHVKWSVEVPLPEATPELLRYARTLAARAAAGA
                     LESPAGPVHLNFPLREPLIPVAPEDYPSTGIERGEPVRVERARRLPDEAALARLADLL
                     LGAERGLIVAGPQPDPALAAPLARLASCLGFPLLADPLSGLRCGSHEPDAIVAGYDAF
                     LRSPRVVEALRPDLVLRVGAIPTSKPVLQFLEAHGAARQILLGGGWEDPSSLACEAVL
                     ADPAATCEAVLRRLDGREISSGWLETWRVLDRASGAAIASYLDGLDEPFEGRVFAELA
                     RLLPEGSTLFASSSMPVRDLDSFFPASRRALRFLANRGANGIDGVVSSALGASVASAG
                     PLLLAIGDIALYHDMNGLLAAKLHN"
BASE COUNT          248 a          802 c          885 g          330 t
ORIGIN      
        1 cgcggcaccc aacccggccc ggcaagtcga ggcgcggccg ggggcggcgt ggcgcggggc
       61 ggtggcgcgg ggtgtggcgg cgatccgggc cggcgagctg gagaaggtgg tgctggcccg
      121 ggcggtgctg ctggaggccg agcgcgcctt cgccgcgccg gccgcgctgg cacgtttggc
      181 cgtcgcctac cccagttgca ctgtcttcgc ttttggccgc gccgggcgca ccttcctcgg
      241 agcgacgccc gagcgactgg cgcgcctgga agacggcgtg ctgcgcaccg ccgcgctggc
      301 cggctcggcg ccacggggag ccacaccgga ggaggacgcg cggctggggc agggcctgct
      361 ggcgagcgcc aaggatcggg gcgagcacgc gatcgtggtg cgcgcattga cggatgggct
      421 ggagggcatc tgcgatgaac tgacgatgcc cggcgaaccg gcgctgctga gtgtggcgaa
      481 catccatcac ctgcacacgc cggtgagtgg gcggctgggg ccgggcaaga ccttgctcga
      541 tgcggtcgag cgcctgcacc cgactccggc ggtcggcggc tggccgcgcg cggcggcgct
      601 gcgcttcatc cgggagcagg aggggctcga ccgcggctgg tacgccgccc cgctgggctg
      661 gctcgaccgc catggcgcgg gcgagttcgc ggtggggctg cgctcggccc tcttggaggg
      721 gtccactgcg acgctcttcg ccggctgcgg catcgtgggc gactccgacc cggccgagga
      781 gtacgccgag tcgcggctca agctacgccc gatgctggcg gcgctgaccg ggtgcgcgcg
      841 atgaggtacg aagaggcggc ctacgtctac ctgggtgcct tcgtcgaggc gctggcgcaa
      901 gcgggcgtgc gcgacgtctg tctggcaccc ggctcgcgct cgacgccgct ggcgctgacg
      961 ctggcccgga accccgccat caaactctgg ctgcagctcg acgagcgctc gaccgccttc
     1021 ttcgcgctgg ggctggccaa ggcgagtggg cgcccggtcg gccttctctg cacctcgggc
     1081 acggccgccg ccaacttctt cccggcggtg gtcgaggcgc actatgcgcg ggtgccgctg
     1141 ctggtcatga ccgccgaccg gccgcccgag ctgcgcgacg cgggcgcggc gcagacgatc
     1201 gatcaggtgc ggctctacgg cgcgcacgtc aagtggtcgg tcgaggtgcc gctgcccgag
     1261 gcgacgccgg agctgctgcg ctacgcgcgc acgctggcgg cgcgggccgc cgccggcgcg
     1321 ctggagtcgc ccgccggccc ggtgcacctc aacttcccgc tgcgcgagcc gttgatcccg
     1381 gtcgcgccgg aggactaccc ctccaccgga atcgagcgcg gcgagccggt gcgggtggag
     1441 cgggcgcggc ggctaccgga cgaggccgcg ctggcgcggc tggcggatct gctgctgggc
     1501 gcggagcgcg ggctgatcgt cgccggcccg cagccggacc cggcgctggc cgcgccgctg
     1561 gcccggctgg cgagttgcct cggcttcccg ctgctggcgg acccgctctc ggggctgcgc
     1621 tgtggttcgc acgagccgga cgcgatcgtc gccggctacg acgccttcct gcgctcgccg
     1681 cgcgtggtcg aggcgctgcg ccccgacctg gtgctgcgtg tgggcgccat cccgacctcg
     1741 aagccggtcc tgcaatttct ggaggcgcat ggcgctgcac gccagattct gctcggtggc
     1801 ggctgggagg atccctcgtc acttgcctgc gaggctgtgc tggccgatcc ggcggcgacc
     1861 tgcgaggcgg tgctgcggcg actcgatggt cgtgagatct cgtccggctg gctggagacc
     1921 tggcgggtac tcgaccgcgc cagcggggca gccatcgcgt cctatctcga cgggttggac
     1981 gagccgttcg agggccgtgt cttcgccgag ctggcgcggc tgctgccgga ggggagtacg
     2041 ctcttcgcca gcagcagcat gccggtgcgc gacctggaca gcttcttccc ggcctcgcgc
     2101 cgcgcgctcc gcttcctggc caaccggggc gccaacggca tcgacggcgt ggtctccagc
     2161 gcgctgggcg cctccgtcgc cagcgccggc ccgctgctgc tggcgatcgg cgacatcgcg
     2221 ctctaccacg acatgaacgg cctgctggcg gcgaagctgc ataac
//
LOCUS       DASUBI010000009         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1226224, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000009 DASUBI010000000
VERSION     DASUBI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226224"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1178)
                     /gene="fabD"
                     /locus_tag="VFU72_00145"
     CDS             complement(288..1178)
                     /gene="fabD"
                     /locus_tag="VFU72_00145"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5421925.1"
                     /translation="MADVAWLFPGQGSQRVGMGRALAASSPTAANVFGAVDEALGFPL
                     SEIIFHGPEDELVATRNQQPALLAVSVAYARVLHERGLLPDPVCLAGHSLGEYSALVV
                     GGALDLADAARLVRRRGELMEAAGLGGMIAVIGLDEPKLAAIASETGVTIANDNAPGQ
                     ITLSGRAEALTAAAERAREAGARRVIPLAVNAAFHSPLMAPVVEGLRPLLAGMRLAAP
                     RAPLIADVDAALLLDPEDLRRELLAQITASVRWVGVVRRAVALGAGVFYELGPGKVLS
                     GLVRRIAPDAETRTAEELLG"
     gene            complement(1180..2184)
                     /locus_tag="VFU72_00150"
     CDS             complement(1180..2184)
                     /locus_tag="VFU72_00150"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5421926.1"
                     /translation="MPNAAITGWGMYVPERVLANAELERLVDTSDDWIVSRTGIRERR
                     LATATDTTVSMALHASRQALAVAGVAPSAIDLIIFATDTPDKLMPASGSLLQAELGAT
                     GAGAFDLDAACSGFGYGLSVGTQFIQSGVFRTVLVVGSDTLTRYIDFRDRGTCILFGD
                     GAGAVVLQASTADEGVLSTVLGSDGRGAEHLYVDGVCTGMVPNGTHDSMPRPYMKMNG
                     GEVFRFAVKVMGEAAIQAVERAGLGLSDIDLFIPHQANERIINASARRLDLPREKVWC
                     NLDRYGNTSAASVPISVAEAAECGALRPGMNVVLVAFGAGLSWAANVVRWGARGVSRD
                     "
     gene            complement(2253..2459)
                     /gene="rpmF"
                     /locus_tag="VFU72_00155"
     CDS             complement(2253..2459)
                     /gene="rpmF"
                     /locus_tag="VFU72_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5421927.1"
                     /translation="MGALPKQRVGRAQQGNRRRHHVLKLPQLMTCPVCGEKKKTHYVC
                     PNCGTYKGRQVLQVAKPRRRDQPE"
     gene            complement(2526..>3015)
                     /locus_tag="VFU72_00160"
     CDS             complement(2526..>3015)
                     /locus_tag="VFU72_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5421928.1"
                     /translation="LKEPVGATRIVDLRLDQFPLDEVARAHDVEAEARLTRVESGVLV
                     DGRVHGLAELECVRCLEPFDAPFAGEFDAEYRPSIDIRTGLPLPRPEDEETFVIDHNH
                     ELDLGELLRQVALLELPMQPICREDCPGIVAGVDAEPEPAAEASDERLAVLRRLLEDE
                     AR"
BASE COUNT          465 a         1106 c         1007 g          437 t
ORIGIN      
        1 ccgtgcgcgt ctccgcacgg agaggggcgc tgcccatcga ctagattggg agccgtgtga
       61 ggacggatcc tgcgacaagc tcaggatgac agggttagaa aggggctgac gaatgcgggc
      121 atcgtcgtcg tacaccccat cccccccatt cacgtcatcc tgagcgcagt cgaacgatcc
      181 gtcctgcctt atcgcgctgc cttctcgatg gccacctccc ctctccgtgc ggagacgtgc
      241 gcggctccgt ccggagaggg gccggtctcc gtgcgggtga ggggccgcta ccccagcagc
      301 tcctccgcgg tgcgggtctc ggcgtccggc gcgatacggc gcaccagccc actgagcacc
      361 ttgcctgggc cgagctcata gaacacaccc gcgccgagcg cgaccgcccg ccgcaccacg
      421 cccacccagc gcaccgaggc ggtgatctgc gccagcagct cccggcgcag gtcttccgga
      481 tccagcagca gggccgcgtc cacgtcggcg atcaatggcg cgcgcggcgc agccaggcgc
      541 atgccggcca gcagcggccg tagcccctcg accaccgggg ccatcagcgg gctgtgaaag
      601 gccgcgttga ccgccaaggg tatcacgcgg cgcgcgccgg cttctcgggc gcgctcggcg
      661 gcggccgtga gcgcctccgc gcgcccgctc agggtgatct gcccgggcgc gttgtcgttg
      721 gcgatagtga cgccggtctc gctcgcgatg gcggccagct tcggctcgtc caggccgatc
      781 acggcgatca tgccgcccag accggccgcc tccatcagct cgccgcgccg ccgcaccagc
      841 cgcgcggcat cggccagatc gagcgccccg ccgaccacca gcgcgctgta ctcacccagg
      901 ctatggcccg ccaggcagac cggatcgggc aagagtccac gctcatgcag cacgcgcgcg
      961 taggccacgc tgaccgccag cagggccggc tgctggttgc gggtggcgac cagctcgtcc
     1021 tccggcccat ggaagatgat ctcggagagc gggaacccga gcgcctcgtc gacggctcca
     1081 aagacgttgg cggcggtcgg cgaggaagca gccagcgctc gccccatgcc gacgcgttgg
     1141 gagccctggc cggggaagag ccaggcgacg tcggccacgc taatcccgcg acacgccgcg
     1201 cgccccccag cgcacgacgt tggccgccca ggagagcccg gcgccgaagg cgaccagcac
     1261 cacgttcatc cccggtcgca gcgcgccgca ctcggcggcc tccgccacgg agatcggcac
     1321 cgaggcggcc gaggtgttgc cgtagcggtc gagattgcac cagaccttct cgcgcggcag
     1381 atcgagacgc cgcgccgagg cgttgatgat gcgctcgttg gcctgatgcg ggatgaagag
     1441 atcgatgtcg gagaggccga ggccggcgcg ctcgaccgcc tggatggcgg cctcgcccat
     1501 caccttgacg gcgaagcgga agacctcgcc cccattcatc ttcatgtagg ggcggggcat
     1561 cgagtcatgc gtgccgttgg gcaccatgcc ggtgcagaca ccgtcgacgt agagatgctc
     1621 ggcgccgcgg ccatcggagc cgagcacggt cgagagcacg ccctcgtcgg ccgtactggc
     1681 ctgcaagacg accgcgccgg cgccgtcgcc gaagaggatg caggtgccgc ggtcgcgaaa
     1741 gtcgatgtag cgtgtcagcg tgtcggagcc gaccaccagt acggtgcgga agacgccgct
     1801 ctggatgaac tgagtgccga cgctcaggcc gtagccgaag ccggagcagg ccgcgtcgag
     1861 atcgaacgcg cccgcgcccg tcgcgcccaa ttccgcctgc aagagcgagc cggaggcggg
     1921 catcagcttg tcgggggtat cggtggcgaa gatgatcaga tcgatggcgc tcggcgccac
     1981 ccccgcgacc gccagcgcct gccgcgaggc gtgcagcgcc atgctgacgg tggtatcggt
     2041 cgcggtggcc aggcgacgct cgcggatgcc ggtgcgcgac acgatccagt catcggatgt
     2101 gtccaccagc cgctcgagct cggcattggc gagcacgcgc tcggggacgt acattcccca
     2161 gccggtgatg gcggcgtttg gcacggtccc gggccacctc tcttacagcg cgagagcgcg
     2221 ggctcatgcc gcgcgctctc gacgattcga ccctattccg gctggtcgcg gcgacgcggt
     2281 ttcgccacct gcaagacctg ccggcccttg taggtgccgc agttcgggca gacgtagtgc
     2341 gtcttcttct tttcaccgca gaccggacag gtcatcaact gcggcagttt gagcacatga
     2401 tgccgccgcc ggttcccctg ctgggcgcgg cccacgcgct gcttcggtag cgctcccatt
     2461 gtttctccct cgaactctct actcttgaaa tagaccgcca ccacgaacgc ggtgcgccgt
     2521 gtggcctagc gcgcttcgtc ctccagcagc cggcgcagca ctgccagccg ctcgtcgctc
     2581 gcctccgcgg ccggctccgg ctccgcgtcc accccggcca cgatccccgg gcaatcctcc
     2641 cggcagatcg gctgcatcgg cagttccagc agcgccacct gacgcaagag ctccccgagg
     2701 tccagctcgt gattgtgatc gatcacgaac gtctcttcgt cctccggacg cggcaacggc
     2761 aggccggtgc ggatgtcgat gctcggccgg tactccgcgt cgaactcgcc ggcaaagggc
     2821 gcgtcgaacg gctccaggca gcgcacgcac tccagctcgg ccagcccatg cacccggcca
     2881 tcgaccagta cgccgctctc cacccgagtc agccgggcct cggcctcgac atcgtgcgct
     2941 cgcgccacct cgtccagcgg aaactgatcg agccgcagat cgacgatccg cgtcgcgccg
     3001 accggttcct tgagc
//
LOCUS       DASUBI010000010         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1239946, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000010 DASUBI010000000
VERSION     DASUBI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239946"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..759)
                     /locus_tag="VFU72_00165"
     CDS             complement(124..759)
                     /locus_tag="VFU72_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5421929.1"
                     /translation="MRLNLTPDELLTTTRSVRKRLDLARPVERAVIEECLRIAQQAPT
                     GSNRQNWSFVVVTDPAKKQGLADCYRRGWLAYTGGQNANPQPGGAQQARVRSSSSYLA
                     EHIGEVPVLVVPCISPRLSPDASVAAQAATFGSIVPATWSFMLAARERGLGTVYTTLH
                     LPHEREAAELLGIPYDEVMQTALIPVAYTKGTGFQPAEREPLATIVHWGAW"
     gene            939..1727
                     /gene="allE"
                     /locus_tag="VFU72_00170"
     CDS             939..1727
                     /gene="allE"
                     /locus_tag="VFU72_00170"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEU5421930.1"
                     /translation="MMTDQRPRRPILPPGIASRGRAIVTGQYALMPAEGILASALPGF
                     RQTTVRILTAPPMGARFAELLLEIAPGGGTTRERDDGLEHLFYVLSGALDLALDGHSH
                     HLSTGGYGYIPVGHRYSLANPSGAECRVIWIKRPYEPIDLLAPGPLVGSRDELPRQSP
                     HTEGRYWQYLLPTDDLAFDMQVNILGFQPGTYFPYIETHIMEHGLYMLEGQGIYLLAG
                     DWHEVEPTDFIWMAPYCPQDFFCTGWGEAAYLLYKDVNRDVRFD"
     gene            1854..>2100
                     /locus_tag="VFU72_00175"
     CDS             1854..>2100
                     /locus_tag="VFU72_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEU5421931.1"
                     /translation="MKIVVCVKQIPDPNLGMHVDPQTKRLQRDPAQSILDPSDEYGLE
                     TALRLVEQHGGEVVAITMGPESAQDALRRAMAMGADRA"
BASE COUNT          326 a          715 c          728 g          331 t
ORIGIN      
        1 tgtcagcctg aggccgcagc cgaaggatct cctccccgtt gcgggtgcct gctatccgtc
       61 accgatgaga gatccttcgc cgcggcctca ggatgataag gggaggcggc gccatcctca
      121 cgtttaccac gcgccccagt gaacgatcgt cgccagcggc tcccgctcgg ccggctggaa
      181 gccggtgcct ttggtgtagg cgaccgggat cagcgcggtc tgcatgacct cgtcgtaggg
      241 gatgccgagc agctcggcgg cctcgcgctc gtggggcagg tgcagcgtgg tgtagacggt
      301 gcccaggccg cgctcgcggg cggccagcat gaagctccag gtcgccggaa cgatgctgcc
      361 gaaggtcgcg gcctgggccg ccaccgaagc gtccggcgac aggcgagggc tgatgcaggg
      421 gaccaccagc accggcacct cgccaatgtg ctcggccagg tacgacgacg acgagcgcac
      481 gcgcgcctgc tgcgcgccgc cgggctgcgg attggcgttc tgcccgccgg tgtaggccag
      541 ccagccacgc cggtagcagt cggccagtcc ctgcttcttg gccgggtcgg tgacgacgac
      601 gaagctccag ttctggcggt tcgagccggt cggcgcctgc tgggcgatgc gcaggcactc
      661 ctcgatgacc gcgcgctcga ccggtctggc caggtcgagg cgcttgcgca ccgagcgcgt
      721 cgtggtcagc agctcgtccg gcgtgagatt cagcctcatc cgtaatgctc ccagatcgct
      781 atcatctatt cgccttgcca cgatcatacg atgaattcgg ccgcgccggg acggcggttg
      841 acgcgagcgc tgccgccgac tagtctccgg cctcagacgc gccagcaggg tgtgcccgag
      901 cgtggctcgg gcggaacgcg ggcttgaacg agggggagat gatgacggac cagcggccac
      961 gacgtccgat cctgccgccg ggcatcgcgt cgcgcggccg ggcgatcgtg acggggcagt
     1021 acgcgctcat gcccgccgaa gggattctcg ccagcgcgct gcccggcttc cggcagacta
     1081 ccgtgcgcat cctgaccgcg ccgccgatgg gcgcgcgctt cgccgagctg ctgctggaga
     1141 tcgcgccggg cggtggcacg acgagggagc gcgacgacgg cctcgagcac ctcttctatg
     1201 tgctgagcgg cgcgctcgac ctcgcgctgg atggccacag ccatcacctg agcaccggcg
     1261 gctacggcta tatcccggtg gggcaccgct actccctcgc gaatccatcc ggcgcggagt
     1321 gccgcgtcat ctggatcaag cggccgtatg agccgatcga cctgctggcg cccgggccgc
     1381 tcgttggcag tcgcgacgag ctgccgcgtc agtcgccaca caccgagggg cgctactggc
     1441 agtatctact gccgaccgac gatctggcct tcgacatgca ggtcaacatc ctcggcttcc
     1501 agcccggcac ctacttcccc tacatcgaga cgcacatcat ggagcacggg ctctacatgc
     1561 tcgaagggca gggcatctac ctgctggccg gcgactggca cgaggtcgag ccgaccgact
     1621 tcatctggat ggcgccgtac tgcccgcagg atttcttctg taccggctgg ggcgaggcgg
     1681 cctacctgct ctacaaggac gtcaaccgcg acgtgcgctt cgactgagcg gatcggttaa
     1741 agatgctgca ccgcccatgc cgcgttgcat tcgcgcgccc gcttccttat gatcggccca
     1801 gcctcgaccg tcggggcggg cgttgagcgc ggagcactgg ataaggaaga cgcatgaaga
     1861 tcgtcgtctg cgtcaagcag atccccgacc ccaacctcgg tatgcacgtc gatccccaga
     1921 ccaagcggct gcagcgcgac cccgcgcagt cgatcctcga cccgtcggat gagtacgggt
     1981 tggagaccgc gctgcgcctg gtggagcagc acggcggcga ggtggtggcg atcacgatgg
     2041 ggccggagag cgcgcaggac gcgctgcggc gggccatggc gatgggcgcc gatcgggcca
//
LOCUS       DASUBI010000011         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_899385, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000011 DASUBI010000000
VERSION     DASUBI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899385"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFU72_00180"
     CDS             <1..1117
                     /locus_tag="VFU72_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5421932.1"
                     /translation="VLEGTIFACEVPTGVLADVRGRRISVVTGLFLIGAGMVLTGIWA
                     RFETILIAQVIWGCGSTFISGAQEAWIADEIGIELAGPVYMRASQVDQLLRILAIPVS
                     IGLATLTLGLPLIVAGTLMPLLGAYLLLAMPEEGFKRELPAGQSFSLRHLGTSTVAGG
                     KLVRASPLLITLFTITIFYGVANQGFDRLWVAQFYDNLGFPHFRSFQPVIWFGVIRFG
                     SAVLSIVAVEYARRHVDMASHHSVSRWLFGITFVQMVSLIVFAMAGSFWVGAIVVWSA
                     ISVSRVYDPLLIAWINQNVPPRVRATVISMSSQTNSIGQISGGPLLGVVSTLTSLRAA
                     LAVSGFVMTPALALYTRAFGQGAAPLVPEAEPETGNP"
     gene            1165..>3105
                     /locus_tag="VFU72_00185"
     CDS             1165..>3105
                     /locus_tag="VFU72_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5421933.1"
                     /translation="MGGLLSTLPVVFRRVAANWKLLAAVVVGAVLAAAIVSTTSIYTD
                     AIRDLGLSYAIRQRGPDQINIVVKSTSQNSAGDVYQKNQGYIESQARRYFGSLIHGDV
                     TQLSRSSTFYPTAPGAAVDPADSRPRSQLQFVTGIEPHINIVAGRLPNDAAPATPNAP
                     PSIEAAVGSDTAKQMGISIGSEYDLHPFWVEDAQPVRVTIVGIIEPKDQSESFWLGQT
                     DLFTFPTSNWQTMPLFITQNTFFNSIVGYLPTMISDFTNLIYLDTSGVNARNADRVKD
                     ALNGYSSDLQSNVLRTTTTTALPDVLATYDQKLFFTRIPLLVLVLQIAAIVMYYLYMV
                     STMLVERQASEIALLKSRGATTWQVMRIYMMEGFFISLAALVLGPPIAAGVIAMLGHT
                     PPFHDLSGGASLRVHLGRDAWLWAAGGALLAYLTLLLPAYQATRRTVVQQRAASARPP
                     KEPVFTRYYLDLALAVLGGILLYQLNRRGTLVNHKFFGDQSVDPVVLLTPAFFILTVG
                     LVFLRLFPLVLKLLAWIVARAQGASILIGMWQLVRNPVHYSRLVLLLMLGTAVGMFAA
                     SFGATLNRSYADRASYQSGSDVRISDVRTVNSAGPNDAPGVIKQDVDGQQVSQVYRLE
                     GSQGPIISRISLDVLGVDPQSFA"
BASE COUNT          517 a         1022 c          937 g          629 t
ORIGIN      
        1 ggtgctggag ggaacgatat tcgcctgcga ggtgccgaca ggcgtgctgg ccgacgtgcg
       61 cggacgtcgt atctcggtgg taaccggcct gtttctgatc ggcgctggca tggtgcttac
      121 cggcatctgg gcccgcttcg agacgatcct gatagcgcag gtcatctggg gctgcggcag
      181 cacattcatc agcggagccc aggaggcctg gatcgccgac gaaatcggaa tcgagcttgc
      241 gggtccggta tacatgcgcg cctcgcaggt cgatcagttg ttacgcatcc tcgcgattcc
      301 ggtatcgatc ggcctcgcca cattgacact ggggctgcca ttgatagtcg ccggtacgct
      361 gatgcctctg ctcggcgcct accttctgct cgcgatgccg gaagagggat tcaagcgcga
      421 gctgcctgca ggccagagct tttccttacg acatctcggc acctctacag tcgctggagg
      481 caagctggtc cgggccagcc cgctactgat cacgctcttt acgattacga tcttttacgg
      541 cgtagcgaac cagggcttcg accggctgtg ggttgctcag ttttacgata acctcggctt
      601 cccacatttc cgctcgttcc agccggtgat ctggttcggc gtgatccgct ttgggtcggc
      661 cgtactcagc atcgtcgccg tcgagtacgc gcgccgccac gtcgacatgg cgagccacca
      721 ctcggtgtcc cgctggctct tcgggatcac ctttgtgcag atggtgagcc tgatcgtgtt
      781 cgcgatggcc ggcagcttct gggtcggcgc catcgttgtg tggagcgcga tttcggtgag
      841 ccgggtttac gacccgttgc tgatcgcatg gatcaaccag aacgtgccgc cgcgggtgcg
      901 cgccaccgtt atctctatgt ccagccagac gaactcgata gggcagatta gcggcggtcc
      961 actgctcggc gtggtgagca cgctgacctc gttgcgcgcc gcgctggccg tctccggctt
     1021 cgtgatgact ccggcgctgg cgttgtacac gcgtgccttc ggccagggcg cggcaccgct
     1081 ggtcccggag gccgagccgg aaaccggcaa tccctgaatt cgattgctga atcagaggcc
     1141 aagtgacaca atgacgcccg aactatgggg ggcctgctta gtaccctgcc cgtcgttttt
     1201 cgccgggtag cggctaactg gaagttgctc gccgcggtgg tagttggcgc ggtgctcgcc
     1261 gccgccatcg tttcgaccac atcgatttac accgacgcga tccgtgacct cggcctttcc
     1321 tacgcgattc gccagcgtgg gcccgaccag ataaacatcg tcgtcaagag cacgtcacag
     1381 aactccgccg gcgacgtcta tcagaagaac cagggctaca tcgaatcgca ggcccggcgc
     1441 tacttcggct cgctgatcca cggcgatgtc acgcagctgt cccgcagttc gaccttctac
     1501 cccacggcac caggcgcggc cgtagaccct gccgacagcc gcccgcgcag ccaactgcag
     1561 ttcgttactg gcatcgagcc tcacatcaat atcgttgcgg ggcgcttgcc aaacgatgcg
     1621 gccccggcca caccgaacgc gcctcccagc atcgaggccg cggtcggctc cgatacggcg
     1681 aagcagatgg gcattagcat cggcagcgag tacgacctgc accccttctg ggtcgaagac
     1741 gcacagcccg tccgggtcac gatcgtgggc atcatcgagc cgaaggacca gagtgaatcg
     1801 ttctggctcg gacagactga cctgtttacg tttccaacct cgaactggca gacgatgccg
     1861 ttgttcatca cgcagaacac gttcttcaac tcgatcgtcg gctacctgcc gacgatgatc
     1921 agcgacttca cgaatctgat ctacctcgac acttccggcg tcaatgcgcg caacgccgat
     1981 cgcgtcaagg atgcgctcaa cggctacagc tccgacctgc aatcgaacgt cctcaggacc
     2041 accacaacga ccgctctgcc cgacgttctg gcgacctatg accagaagct gttcttcacg
     2101 cgcatcccgc tgctggtgct ggtcctgcag attgcggcaa tcgtcatgta ctacctctac
     2161 atggtttcga cgatgctggt ggagcggcaa gcgtccgaga tcgcgttgct caagagccgc
     2221 ggcgctacga cgtggcaggt gatgcgcatc tacatgatgg agggcttctt catctcgctg
     2281 gccgcgctcg tgctcggtcc gccgatcgcc gcgggcgtga tcgccatgct cggtcacacg
     2341 ccgccgttcc acgatctgag cggcggggcg agtttgcgcg tacaccttgg tcgcgacgcg
     2401 tggctctggg ccgcgggtgg cgcattactc gcctacctga cgctgcttct gccggcttac
     2461 caggcaacac gacgtactgt cgtacagcag cgcgccgcga gcgcccggcc gccgaaggaa
     2521 ccagtgttca cccgctacta cctggacctg gcgctcgcag tgctcggcgg catcctgctt
     2581 taccagctca accgtcgcgg gacactcgtc aaccacaagt tcttcggcga ccagtcagtc
     2641 gaccccgtcg tgctgctgac gccggcgttt ttcatcctga ctgtcgggct ggtcttcctg
     2701 cgcctgttcc cgctcgtgct caagctactc gcgtggatcg tcgcccgtgc gcagggggct
     2761 tcgatactca tcggcatgtg gcaactggtg cgcaacccgg tgcactactc gcgcctggtg
     2821 ctgctcctca tgctcgggac cgccgtaggc atgttcgcgg cgagctttgg cgccacgctt
     2881 aaccgcagct acgccgaccg cgcctcgtac cagtcgggct cggacgtgcg catcagcgac
     2941 gtgcgcactg ttaattccgc cgggcctaac gacgcacccg gcgtaatcaa gcaggatgtc
     3001 gacgggcagc aggtgtcgca ggtctatcgc ctcgagggta gccagggccc gatcatcagc
     3061 cgcatttcgc tcgacgtgct gggtgtcgac cctcagagct tcgcc
//
LOCUS       DASUBI010000012         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1280760, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000012 DASUBI010000000
VERSION     DASUBI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280760"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFU72_00190"
     CDS             <1..209
                     /locus_tag="VFU72_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421934.1"
                     /translation="AGRPREAVAVYGAMSIARVPEAEWAARAEAWRTFGATHFLIETV
                     GVGLESPAEQIRVLSRIKEAIELA"
     gene            362..1567
                     /locus_tag="VFU72_00195"
     CDS             362..1567
                     /locus_tag="VFU72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF042684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6545 domain-containing protein"
                     /protein_id="HEU5421935.1"
                     /translation="MSRLSVLLLLGLMAYAALIWVLLTTRLWSRRRHELTAVDYVASC
                     CGFLALGLMLTTPLPPVAAEVDRLAGRVGVAEWLADVGALLAALGWVVYLSRLVPPEQ
                     RWYRVRRTDRWVPLLPVTALTAVALAVGAGRFIWAPGRLGYHFGPQPDPGRTYLVAAH
                     LVYRATLLVDLALLIPVLRRLGEVAAGTPVLRARLQAIRGWSWYTIGHISYECATIFL
                     PLPDWMVYTRRQMSLFLVLAIIAPDWWYEQGLALLDQVRDTVWRWQTHWSRWRDYQRL
                     YPFWAALYPVQPGVSLLDPPQDRAPRPDRPKLTFTVYRLGIEIDDWRTRLYPYRPVPV
                     APGPPTAATEAWAVATALRAWQAGTPATPDRPPGQAEPELYGGATMEEEIAALVRVAV
                     VFNRIMRNL"
     gene            1659..>2369
                     /locus_tag="VFU72_00200"
     CDS             1659..>2369
                     /locus_tag="VFU72_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5421936.1"
                     /translation="MDECATTIAVYGERDHLYALAAAVPRWADFLPHYRWTRPAAPSE
                     DGAAADLDMACWRGWIPLRWRTTVTTDPATHRVQFVHTGGGARGMQVEWRLVEQDGTT
                     TATITHDLRGLAQWPATTPPGRYILTRHLIEPVARRTLATMKTLVEAGATDPDTAVAL
                     AADPAGPVPARDDPRAELLSWLPWALWLGTSVVWSRWSRRTLAASGESRRREEPAGAW
                     RGHLAAFAATFALLAPALP"
BASE COUNT          330 a          837 c          830 g          372 t
ORIGIN      
        1 ccgcgggccg accacgcgag gcggtggcgg tgtacggggc gatgagcatt gcgcgcgtgc
       61 ccgaagcgga gtgggcggcg cgggcggagg cgtggcgaac gttcggggcg acacacttcc
      121 tgatcgagac ggtgggggtc ggcttggagt cgccggccga acagatacgc gtgctctcgc
      181 ggatcaagga ggccatagag cttgcctgat cccgcacgcc actgcgtagt gagctgcagg
      241 ctagcgtcac ctgccaggag tgagtgcgaa ttcgtgtccg agaggctacg tcgaggattg
      301 atacaggcgg ccaggacgac gagcaatatg tgcaaaggag gtcctgggga ggcgtgatgc
      361 aatgagtcgt ctttctgtgc tcttgctcct tggtctcatg gcttatgccg cgctgatctg
      421 ggtattgctc accacccggc tctggtcgcg ccgccgccac gagttgacgg cggtcgacta
      481 cgtcgcgtcc tgctgcggct tcctggcgct tggactgatg ctgacgacgc ccctgccgcc
      541 ggtcgccgcc gaggtggatc gcctggccgg ccgcgtgggc gtagcggagt ggctggccga
      601 tgtcggagcg ctcctcgccg cgctcggctg ggtcgtctac ctgtcccggc tcgtgccgcc
      661 cgagcaacgc tggtatcgcg tgcgccgcac cgatcgctgg gtgcccctgc taccggtgac
      721 cgcgctgacc gcggtggcgc tcgccgtcgg cgccgggcgc ttcatctggg cacccggccg
      781 gctcggttac catttcgggc cgcagcccga ccccggcagg acctacctgg tcgccgccca
      841 tctggtctat cgcgccacgc tcctcgtcga tctcgcgctc ctcatcccgg tgctgcgccg
      901 gctgggcgaa gttgccgccg gcacgccggt cctccgggcc cggttgcagg cgattcgtgg
      961 ctggagctgg tacaccatcg ggcacatcag ctacgagtgc gccaccatct tcctgcccct
     1021 cccggactgg atggtctaca cgcgccggca gatgtccctg ttcctcgtgc tggcgatcat
     1081 cgcgccagac tggtggtacg agcagggact cgcgctcctc gaccaggtgc gcgacaccgt
     1141 gtggcgctgg cagacgcatt ggagtcgctg gcgcgactac cagcgactat acccgttctg
     1201 ggcggcgctc tacccggtgc agcctggggt ctccctcctc gatccgccgc aggatcgcgc
     1261 cccgcgtccc gaccggccca aactcacctt taccgtctac cggctgggaa tcgagattga
     1321 tgactggcgc acgcgcctgt acccgtaccg gcccgtgccc gtcgcacccg gaccgccaac
     1381 tgccgccacc gaggcctggg ccgttgccac cgccctacgc gcatggcagg cgggaacccc
     1441 ggcaacgccg gaccgcccgc ccggccaggc cgagcccgag ctgtacggtg gggcgaccat
     1501 ggaggaagag atcgcggcgc tcgtgcgggt cgcggtcgtg ttcaaccgga tcatgcgcaa
     1561 cctgtaggct ggcactgacg cgtcgaggat gatgcgcccg gagaaccacg acggcacccc
     1621 gctggaatgc ggaccgaaaa gcggataagg agtaacggat ggacgagtgc gcgacgacaa
     1681 tcgcggtgta tggcgagcgc gaccacctct acgcgctggc ggcggcagtg ccccgctggg
     1741 cggacttcct gccacactac cgctggacgc ggcctgcggc gccgagtgag gacggcgcgg
     1801 ccgcggacct ggacatggcc tgctggcgcg gctggatacc actgcgctgg cgcaccaccg
     1861 tcaccaccga tccggcgacg caccgcgtgc agttcgtcca taccggcggc ggggcgcggg
     1921 gcatgcaggt cgagtggcgc ctggtggagc aagatgggac gacgaccgcg actatcacgc
     1981 acgacttgcg cggcctggcc cagtggccgg cgaccacgcc gcccgggcgc tacatcctca
     2041 cccgccacct gatcgagccg gtcgcccggc gcaccctggc gacgatgaaa acgctggtgg
     2101 aggcgggcgc caccgatccc gacaccgcag tggcgctggc ggctgacccg gccggaccag
     2161 taccagcccg cgacgatccc cgcgcggagc tgctctcctg gctcccctgg gcgctctggc
     2221 tgggtacgag cgtggtctgg agccgctggt cgcgccgcac gctcgcggcc agcggcgagt
     2281 cgcgccgccg ggaggagccc gcgggcgcct ggcgcggtca tctagccgcc ttcgctgcga
     2341 ccttcgcctt gctcgccccg gcccttcca
//
LOCUS       DASUBI010000013         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_558485, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000013 DASUBI010000000
VERSION     DASUBI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558485"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      153..295
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            410..1477
                     /locus_tag="VFU72_00205"
     CDS             410..1477
                     /locus_tag="VFU72_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEU5421937.1"
                     /translation="MRRILLALSLCLALALLAAPATLAAGPDTTSAVAWLRQQQQPDG
                     GFAGLNGKTDPSTTADVALALSAAGVDPAGVATSGKSVLDFLRANAAGYGASTGGAAK
                     LALAAEAAGLDPRSFGGTDLIGKLQSAYDAKTGLYDAQVYVDGLAVLALHGAGASLGT
                     DAVKGLTSHQAADGSWAFDGDTAPGKGDSNTTAIVLQALVAAGASGSPAIQHGIAYMT
                     SARTPDGAYVSQPGQENPPVGDANSTALAIESLLATGQSIDSPSVSAALTALRALQNP
                     SGAFAYRPDAKQDNLLATVQAIPALAGWTYLQPRSGGVAGTVAPATGGARAGVPLVAL
                     AAGLALAALALGGTLRLRATD"
     gene            1477..2256
                     /locus_tag="VFU72_00210"
     CDS             1477..2256
                     /locus_tag="VFU72_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421938.1"
                     /translation="MRRARLARLALLLAVFGWLVPLTAIAAGPTPDAVPAALHGAGLV
                     VRHGDGRVLYLYVPFDEPSISGAELLQRSRLALTLSPFAGLGVAVCSLDNEGCPASDC
                     FCKSYGQPSIYWHFYRLAPDGKWAPSALGPSEITIHPGDIEGWGWDSGDNQLPPTSFR
                     DIARLNGLLPVATPHATAAPTGATPEPATSAVAAVATSVGTAEARGVEVAPGGTVRAV
                     QPAPHEGGPRTGTLVLFGALVVLVLLAGLAAVLRRRARRAP"
     gene            2253..3266
                     /locus_tag="VFU72_00215"
     CDS             2253..3266
                     /locus_tag="VFU72_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HEU5421939.1"
                     /translation="MSQSNALAWLAWAGAAALLVISSRNPLLLTLVLLETALVYLSGD
                     RGGLRGAAWSAVLRIGLAVAGLSIAFNLLTAHVGDHVLVTLPGAIPIAGGPLTLNSLL
                     YGAVSGAAIVTLLLIAACFNARVERAALLRLVPGPLLTSGMAAIVALSFFPQTLQALS
                     EVREAQAARGFRLRSPRDLNVLIVPVLHLGLEHAFKLAETMESRGFGASRAAPARHAR
                     LLPVSLLTALSGITLLLMGARVAGLAVLIAALVLLGVGLLRAPHSRRGSLRPLPWNAA
                     NLALLATALAALALTALAITAAPAALEWSPYPGLNWPALGLLPALACLLLAAPLVLEA
                     LIP"
     gene            3263..>4246
                     /locus_tag="VFU72_00220"
     CDS             3263..>4246
                     /locus_tag="VFU72_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5421940.1"
                     /translation="MIRFQSVSYRYPGQQEPALADFSWEVPEGAVTLVAGPSGCGKST
                     ILRCPNGLIPNFYGGGFGGSVLTFGLDTRLHDTAALSRLAGFVAQEPETQTVTDRVED
                     EIAFGLENAGLARREIQARVDELLEALGIAHLREREIGTLSGGERQRVVIAAACAPRP
                     RLLALDEPTSQIDPWSAPDVVDAFTRLNRDDGTTILLAEHRLARVLPAADRVLLLEAG
                     GRLVADGPPIGVARARDEVWPSPKVAATTPTGPAAVELEAVTHRYGQHAALTDVSLAF
                     GAGQVTALMGRNGSGKTTLLKHLNGLLRPDEGRVRLLGAEIARRPTHDLARQ"
BASE COUNT          546 a         1606 c         1463 g          631 t
ORIGIN      
        1 gttcctccat gacccgattc aaaccaactc cgcgtgatga tgcgtgccgg cgggcccaac
       61 cgtcaatcgc cccctggccc actcgttacc tcgaccttgc acgagccatg agttcgtgcc
      121 acactgcgcc tgccggcatc acggcgccgg cttgatcgac ctgcggcagc ggttgccggt
      181 cgctttgacc ggcggtcgcg ggagaacacc ggtgtgaatc cggggctgtg ccgcaactgt
      241 gacgccgcgc tcgcgcggcg aagccagatc gcccgccgct gtcgcgtccg ctcactcctc
      301 gagccaagga ggcaggacgc ccatgtccca cccaatctcc ccttgagacc gcctctcgtg
      361 tgacttcagc cctggctgca ttgtctggaa gacgcactgg aggttgcgca tgcgccggat
      421 tctccttgcc ctttcgctct gtctcgcgct cgccctgctc gccgccccgg ccacactggc
      481 cgccggaccg gacaccacgt ccgccgtggc ctggctgcgc cagcagcagc agccggacgg
      541 cggcttcgcc gggctcaacg gtaagaccga ccctagcacg acagccgacg tcgcactcgc
      601 gttgagcgcc gccggcgtcg atccggccgg cgtcgccacg agcggcaaga gcgtgctcga
      661 cttcctgcgc gccaacgccg ctggctacgg cgccagcacc ggcggcgccg ccaagctggc
      721 gctggccgcc gaggcggccg gactcgatcc gcgatccttc ggcggcaccg atttgatcgg
      781 gaaactacaa tccgcgtacg acgcgaaaac cggcctctac gatgcgcagg tctacgtcga
      841 cgggctggcc gtgctggcgt tgcacggagc cggcgcgtcg ctcggcaccg acgcggtgaa
      901 ggggctcacg agccaccagg ccgccgacgg cagttgggcc ttcgacggcg acaccgcgcc
      961 gggcaagggc gacagcaaca ccaccgccat cgtcttgcag gcgctcgtcg ccgcgggcgc
     1021 cagcggctcg cccgccatcc agcacggtat tgcctacatg acgagcgcgc gcacgccgga
     1081 cggcgcctac gtttcccagc ccggccagga gaaccccccg gtcggcgacg ccaactcgac
     1141 cgcgctggcg atcgagtcgc tgctcgcgac cggccagtcg atcgattcac cctcggtcag
     1201 cgcggcgctg accgccctgc gcgccttgca gaatcccagc ggcgccttcg cctaccgccc
     1261 ggatgccaag caggacaacc tgctggcgac cgtgcaggcc atcccggcgc tggcgggctg
     1321 gacctacctc cagccgagat ccggcggagt cgccggcacg gtcgcgccgg ctacgggtgg
     1381 cgcgcgggct ggcgtcccgc tcgtggcgct ggccgccggg ctggcattgg ccgcgctggc
     1441 gctcggcggt acgctgaggc tgcgggcgac ggactgatgc gccgcgcccg tctcgcccgt
     1501 ctggcgttgc tgctcgcggt gttcggctgg ctggttccgc tgacggcgat cgcggccgga
     1561 ccgacgccgg acgcggttcc ggccgcgctg catggcgccg ggctcgtcgt gcgccacggc
     1621 gacggccgcg tgctctatct ctacgtcccc ttcgacgagc cgtcgatcag cggtgcggag
     1681 ctcctgcagc gctcgcgcct cgccctcacg ctcagcccct tcgccgggtt gggcgtggcc
     1741 gtctgcagcc tggacaacga gggttgcccg gccagcgact gcttctgcaa gagctacggg
     1801 cagccctcga tctactggca cttctaccgg ctcgcgcccg acggcaagtg ggcgcccagc
     1861 gcgctcggcc cgagcgagat caccatccat cccggcgata tcgagggctg gggctgggac
     1921 tccggtgaca atcaactccc accgacaagc tttcgcgaca tcgcgcggct gaacgggtta
     1981 ctgccagtgg ccacgccgca cgcgacggca gcgccgaccg gcgcaacgcc ggagccggcc
     2041 acatcagcgg tcgcggcggt agcgacgagc gttggcacgg ccgaagcgcg cggggtcgag
     2101 gtcgcgccgg gcgggacggt gcgcgccgtt caaccggcgc cgcacgaagg cggcccgcgc
     2161 actggcacgc tggtcctctt tggtgcgctg gtcgtgctgg tgctgctggc gggcctggct
     2221 gcggtgctgc gacgccgagc gcggcgggcg ccgtgagcca gtcgaacgcg ctcgcctggc
     2281 tagcctgggc cggcgcggcg gcgctgctgg tcatcagcag ccgcaacccg ctcctgctca
     2341 cgctggtgct gctggagacg gcgctcgtct atctcagcgg cgatcggggc ggcctgcgcg
     2401 gcgcggcctg gtcggcggtg ctgcgcatcg gcctggccgt cgccgggctc agcatcgcct
     2461 tcaacctgct cacggcgcac gtcggcgacc acgtcctggt gacgctcccc ggcgccatcc
     2521 cgatcgccgg tggcccgttg acgctgaact cgctcctcta cggcgcggtc tcgggtgcgg
     2581 ccatcgtaac cctgttgctg atcgcggcct gcttcaacgc gcgcgtcgag cgtgcggcgc
     2641 tgctgcgcct ggtgccgggg ccgctcctga ccagcggcat ggccgccatc gtagcgctga
     2701 gcttcttccc ccaaacgctc caggcgctga gcgaagtgcg cgaggcccag gcggcgcgcg
     2761 gcttccgcct gcgctcaccg cgcgacctca acgtgctgat cgtgccggtg ctgcatctgg
     2821 gtctggagca cgccttcaag ctggccgaga cgatggagag ccgcggcttc ggcgcgagca
     2881 gggcggcgcc ggcgcgccac gcccggctcc ttccggtgag tctgctgacg gcgctctccg
     2941 ggatcacgct gctgctgatg ggcgctcgcg tcgccggact ggccgtgctg atagcggcgc
     3001 tggtgctgct cggggttggc ctgctgcggg cgccgcacag ccggcgcggc agcctccgtc
     3061 cgcttccctg gaacgcggcc aacctggcgc tcctggccac cgcgctggcc gcactggcgc
     3121 tgaccgcgct ggcgatcacg gcggcccccg ccgcgctgga gtggtcgccc taccccggct
     3181 tgaattggcc ggcgctcggc ctcctcccgg cgctcgcctg tctcctgctg gcggcgccgc
     3241 tggtgctgga ggcgctcatc ccatgattcg cttccaatcc gtcagctacc gctacccggg
     3301 ccagcaggag ccggcgctgg cagacttctc ctgggaggtg cccgaaggcg ccgtcaccct
     3361 ggtcgcgggc ccctccggct gcggcaagtc aaccatcctg cgctgcccca acgggctgat
     3421 cccaaacttc tacggcggcg gcttcggtgg cagcgtactc accttcggac tcgacacccg
     3481 cctccacgac accgccgcgc ttagtcgcct ggccggcttc gtggcgcagg agccggagac
     3541 gcagacggtc accgaccggg tcgaggacga gatcgccttt ggcctggaga acgccgggct
     3601 cgcgcgccgc gagatccagg cccgtgtcga cgagctgctg gaggcgctcg gcatcgcaca
     3661 cctgcgcgag cgcgagatcg gcacgctctc gggcggcgag cgccagcggg tcgtcatcgc
     3721 ggccgcctgc gccccgcgcc cgcgcctgct ggcgctcgac gagccaacct cacagatcga
     3781 cccatggtcg gcgcccgatg tcgtggacgc cttcacccgg ctcaacaggg acgatggcac
     3841 gactatcctg ctggccgagc accgcctggc gcgtgttctg ccggccgccg accgcgtcct
     3901 cctgctggaa gcaggcggcc gcctcgtggc ggacggaccg cccatcggtg tcgcccgcgc
     3961 gcgcgacgaa gtctggccgt cgccgaaggt ggcagccacg acgcccaccg gcccggcggc
     4021 ggtcgagctg gaggccgtca cccaccgcta cggccagcac gccgcgctta cagacgtcag
     4081 cctcgcgttc ggcgcgggac aggtcacggc attgatgggg cgcaacggca gcggcaagac
     4141 gacactgctc aagcacctca acggtctgct gcgcccggac gaaggacgtg tgcgcctgct
     4201 cggcgcggag atcgcccgtc gtccgacgca cgatctcgcg cgccag
//
LOCUS       DASUBI010000014         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_844829, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000014 DASUBI010000000
VERSION     DASUBI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844829"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..784)
                     /locus_tag="VFU72_00225"
     CDS             complement(332..784)
                     /locus_tag="VFU72_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5421941.1"
                     /translation="MSTTLLEREPVLAREEERPTLKRIAEGFQAGQVAEEGKVRVTFG
                     AESVELPESLVRLIQQVVNVLVQGEAVSIVPYHKDLTTQEAADILNVSRQYLVRLLEK
                     DEMPYHMTGTHRRIRFGDLMEYKRRRDATRRRGLAKLTQQSQKLGLYQ"
     gene            1047..1529
                     /locus_tag="VFU72_00230"
     CDS             1047..1529
                     /locus_tag="VFU72_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421942.1"
                     /translation="MSPIFVVAIYFLAWRQCRRIGQAIAETAAWIVGYLGTYVILVVL
                     HFGRPSLVSLSPESFSFVFAVIGGFVVMKLSHWLWRRRGGHLEPVKHPFAAKHWMRAL
                     RAALFGGKPRQRASGATVTRAASTTGASRPARPRQTNRWVRVARAMLDTESSRGRTRR
                     "
     gene            1589..1966
                     /locus_tag="VFU72_00235"
     CDS             1589..1966
                     /locus_tag="VFU72_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5421943.1"
                     /translation="MIQMKRIYEPESPDDGYRVLVDRLWPRGVKKTEAHLDAWDKELA
                     PSTELRKWYGHDPEKWPEFQKRYRQELQQPESQQILQDLAGRARHGTVTLLYAARAGQ
                     ISEAAVIRDVLDAMLGQPARSEG"
     gene            complement(2286..2432)
                     /locus_tag="VFU72_00240"
     CDS             complement(2286..2432)
                     /locus_tag="VFU72_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421944.1"
                     /translation="MNVAAIVSAMLIIALGITLLGVIVISLLAIAGRLLFAHLGHEPA
                     EAPR"
     gene            complement(2524..>3492)
                     /gene="murB"
                     /locus_tag="VFU72_00245"
     CDS             complement(2524..>3492)
                     /gene="murB"
                     /locus_tag="VFU72_00245"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5421945.1"
                     /translation="AMLVERDVPLSDVVWYRIGGPARVLLSVRDAPGLERAIAFVERQ
                     RPPRLLVIGRGSNLLMPDGPFDGIVIRLLGGKLPRNASRQARTREVFGGALLNDLARS
                     TLEDGLTGLEWAGGLPGTVGAALRGNAGAFGGTMADRVRRVTVVALEAGGCQLLELDR
                     AAMRFGYRDSLLKHESDWIVLRASFELQPAAAADLEEAWRAYQRHVAYRRRRHPLEYP
                     NCGSVFKNLTEPAEIARVLERWPDLSEQVRDEWHGKVPVAAVIARLGLAGLTVGGAQL
                     STRHQNFIVNLGGATAADVRAVIARVRDAVECALGLNPQLEIELID"
BASE COUNT          562 a         1224 c         1141 g          565 t
ORIGIN      
        1 gatatggttt gcggctccat ttttcgggct catgaggaga acgataggcg cttcatcgaa
       61 atcattcagc cgcgcatcga ggatactcgg aattggcctt aaaccggaga ccccacgcta
      121 tcccggatcg aatccgagaa cgccacccct acggcagctg ttcgcgtggg atctcaatgg
      181 ctctatctat attgtcgcga ccgtgtcggc cgcgtgcgta gtatacctat gtagtcgatc
      241 aatgtccagt cgtacatcga cgtctgaggg cgcagcgctg ctgactctag cacgcaggca
      301 tccacaacca ccaacggggc accctcaccg cctactgata caggcccaac ttctggctct
      361 gctgcgtaag ctttgccaat cccctgcgac gcgttgcgtc ccttcggcgc ttgtactcca
      421 tcaggtcacc gaagcggatc cgccggtgcg tgccagtcat gtgatagggc atctcatctt
      481 tctctaggag gcgcaccaga tactggcgtg agacgttgag aatgtcggct gcctcctgtg
      541 ttgtcaggtc tttgtgatag ggaacgatgg agacggcttc accctgcacg aggacattca
      601 cgacctgctg gatgagccgc actagggact ccggtagctc gaccgactca gcaccgaatg
      661 tgactcgaac ctttccctcc tccgccactt gcccagcttg gaagccctcc gcgattcgct
      721 tgagcgtcgg acgttcttcc tctcgcgcga gaacaggttc tcgctccaat agggtggtgg
      781 acatccggtt ctccttcttc ctgcctcggc acccgcccgt agacgccgct ctacgccatc
      841 atagccggta aacgctataa acgcaactat taggccagca ccgttggtca cgcctagcca
      901 agtacgatgg gcaatacggc tcgaaccacc cagaattatc ctgtaccgag tcgaggtcgg
      961 cggtagcgag cggccgcgtg gcgggagtgg cgcggattga gtaggcttac cggtgttcgt
     1021 cgccgccatg tggggtgagg aagcgcgtgt caccgatctt cgtcgtcgca atctacttcc
     1081 tggcctggcg ccagtgccgc cgcatcgggc aggcgatcgc cgagaccgcc gcctggatcg
     1141 tcggctacct gggcacctac gtcatcctgg tcgtgctcca cttcggccgc ccgagcctgg
     1201 tctcgctgag cccggagagc ttctccttcg tcttcgcggt catcggcggc ttcgtcgtca
     1261 tgaagctgag ccactggctc tggcgccgcc gcggcggcca cctggagccg gtcaagcacc
     1321 ccttcgccgc caagcactgg atgcgtgccc tgcgcgccgc gctcttcggc ggcaagccgc
     1381 gccagcgggc cagcggcgcc acggtgacgc gggcggcgag cacgaccggc gccagccggc
     1441 ccgcccgccc gcgacagacc aaccgctggg tgcgcgtcgc gcgtgctatg ctcgacaccg
     1501 agagcagccg cggccgcacc cgccgctgac gcgccttccg tgggttcgat cggcggcagc
     1561 cgggcggcgc ggggaaggca gtagcaccat gatccagatg aagcgcatct acgagccgga
     1621 atcgccggac gatggctacc gcgtgctggt cgaccggctc tggccgcgcg gggtgaagaa
     1681 gaccgaggcg cacctcgacg cctgggacaa ggagctcgcg ccctcgaccg agctgcgcaa
     1741 gtggtacggg cacgatcccg agaagtggcc ggagttccag aagcgctatc gccaggagct
     1801 gcagcagccg gagtcgcagc agatcctgca ggatctcgcg gggcgggcgc gccacggcac
     1861 ggtcacgctc ctctacgccg cgcgcgccgg tcagatcagc gaggccgcgg tcatccgcga
     1921 cgtgctggac gccatgctgg ggcagccggc ccgatccgag ggctgagccg ccgcgctagt
     1981 cgccggctgc ggaatcggac ggctgccgtg cgcggccgga ttcctccgtg gcgtggttgg
     2041 gcttggggga gagcaggtgg ctggcgagga gcacgggtgc atgtaaggac ggatccctcc
     2101 gcttcggtcg ggatgccagg ggtagtgtcg ggctgacgag gaggatcacc gtcggacgag
     2161 gatgcgccgc gcccggtagg tcatcccctt tccccggtga tcccttcggc aggctcaggg
     2221 cgggctctgc gcgcaggcga aggatccgtc cccacacagc acccatcgtg tccgtcagac
     2281 agcccctagc gcggcgcctc cgccggctcg tgtcccaagt gcgcgaagag cagtcgcccg
     2341 gcgatggcca gcaggctgat aacgatcacc ccaagcagcg tgatgccgag cgcgatgatc
     2401 agcatcgcgg agacgatggc cgccacgttc acggtgggtt ccccttctac ccatggtcga
     2461 cggcgcgggc gagacgcgct cgcccgcatt gtacccgcac ccacaccccc gcaccgctcg
     2521 cgctcagtcg atcagctcga tctccagttg tgggttgagg ccgagggcgc actcgaccgc
     2581 gtcccgcacc cgcgcgatga ccgcccgcac gtccgccgcc gtcgcgccgc cgaggttgac
     2641 gatgaagttc tggtgccggg tcgagagctg cgcgccgccg accgtgagcc cggccagacc
     2701 cagccgcgcg atgacggcgg cgaccggcac cttgccatgc cactcatcgc gcacctgctc
     2761 cgacaggtcc ggccagcgct ccagcacgcg cgcgatctcc gcaggctccg tcaggttttt
     2821 gaagaccgag ccgcagttgg ggtactccag cgggtggcgc cggcgccggt aggcgacgtg
     2881 ccgctggtag gcccgccagg cctcctccag atccgccgct gcggctggct gcagttcgaa
     2941 gctcgcccgc agcacgatcc agtcgctttc atgcttcaaa aggctgtcgc ggtagccgaa
     3001 gcgcatggcc gcacggtcga gctccagcag ttggcagccg cccgcctcca gcgcgaccac
     3061 cgtgacgcgg cgcacgcgat cggccatcgt gccgccgaag gccccggcgt tgccccgcag
     3121 ggccgcgccg accgtgcccg gcaagccgcc ggcccattcg agcccggtca ggccgtcctc
     3181 tagcgtcgag cgggccaggt cgttgagcag cgcgccgccg aagacctcgc gggtcctcgc
     3241 ctgccggctc gcgttacgcg gtagcttacc gcccagcagc cggatgacga tcccgtcgaa
     3301 cggaccgtcc ggcatgagca gattcgagcc gcgcccgatc accagcaggc gcggcgggcg
     3361 ctgccgctcg acgaacgcga tggcccgctc cagccccggc gcgtcgcgca ccgagaggag
     3421 gacgcgcgcc gggccgccga tgcgatacca gacgacgtcg gacaggggca cgtcgcgctc
     3481 caccagcatc gc
//
LOCUS       DASUBI010000015         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1321423, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000015 DASUBI010000000
VERSION     DASUBI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321423"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFU72_00250"
     CDS             complement(<1..192)
                     /locus_tag="VFU72_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421946.1"
                     /translation="MASPASVLDLSRLVRGGLAVVATLLLAFLLAGAFVVATLTAALS
                     ALNPARPAGNGNGTSQAIPA"
     gene            complement(203..1387)
                     /locus_tag="VFU72_00255"
     CDS             complement(203..1387)
                     /locus_tag="VFU72_00255"
                     /inference="COORDINATES: protein motif:HMM:NF039657.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL family protein"
                     /protein_id="HEU5421947.1"
                     /translation="MSPRDARARTLLPAVRRSPWQHVLIAVLGRMGLLGIALGALGVG
                     GAAPTVAATIAFRQPVAPREQPVAAIIVAAARTSSAAVLDQHLGVGLAGGASALQREV
                     AVPLPPILPPSPIDPQLIKNLFLTMAGEALQEVNAALRAPLDAWLASPLNVVSQTPPA
                     GSYASGVVTVLWRVVRGAANALLVVVVVGGGLNVMLKDRIGAPYHEAAELLPRAAVGA
                     LLANTSLWLAQLAIDLNNGLCGLIGGASLPGWQQAGQPLQGIASAVAGLLYLITALFL
                     ILQMLMRLALIDVLIVVAPLALLCWTLPQTQGWGRRWSAAYTSAVFTQFVQIVALKLG
                     AELFTDLTPASSAGEALLPSLVGIALLVLTMRLPGLLGLRGGSGGSGVLRYIAYQQLR
                     GR"
     gene            complement(1384..2847)
                     /locus_tag="VFU72_00260"
     CDS             complement(1384..2847)
                     /locus_tag="VFU72_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU5421948.1"
                     /translation="MELPFIIRVKLPSRTGWWHPWVWLLIGLPVVLGVVRLAQQRQPA
                     ATSPGPSAMLVLASNPPGAAIKIDDKSRGQTPATLALPPGAHRLTLRDTGRTDVTYTV
                     HLTANATTDLTGILWLRTPAVRPVRSPLPGATIAGTVFLADGRLALALALPAGDERQV
                     WIVGQDGATQRVGPPFARGAAAVSPDGTQVAYLAQPSASAAPGAPASDGAGGRLTEVW
                     VTDRNGDSGQRRYVLPPNTAAERLVDLAWAPDSTQLLLVSEQHPQGSGVRQRLQWLNL
                     ATAPPATRELLTLPGQVVPGSYLWNTAGDRLALLVRADNRTTLCLLGRDGQFFRSLAD
                     VPAAGAPPAFSPLVWLPKEGGVVYAAAAPGRQAAVWPTFASPAPDLLVDDLTAGGSRR
                     LPGTGGWGPGWRDDGFALALTRPKRNGPLTVQAFDLTTDTAAQTLGTLALPADRVAGV
                     RWDMPHGQALVAIASATGGPPDLWLVTWTTHDEEGGR"
BASE COUNT          503 a         1029 c         1122 g          421 t
ORIGIN      
        1 ggccggaatg gcctgggacg tgccattgcc gtttccggcg gggcgtgccg ggttcagcgc
       61 gctgagggct gcggtcaggg tggcgaccac gaacgcgccg gccagcagga aggccaggag
      121 cagggtggcc acgaccgcca gaccgccgcg caccagccgc gaaagatcca gtacactcgc
      181 cgggcttgcc atcgcgcccc cctcaccggc cgcgcagttg ctggtaggcg atgtagcgga
      241 gtacgccgct cccgccacta ccacctcgca ggccgagtag gcctggcagg cgcatcgtta
      301 gcaccaggag cgcgatgccc accaggctcg gcaacagcgc ctcgcccgcg ctggacgccg
      361 gggtgaggtc ggtgaagagc tcggcgccga gcttgagcgc gacgatctgc acgaactggg
      421 tgaagaccgc gctggtgtag gcggccgacc agcggcgccc ccagccctgt gtctgcggca
      481 gcgtccagca caggagcgcc agcggggcga ccacgatcag cacgtcaatg agagccaggc
      541 gcatcagcat ttgcaggatc aggaagaggg cggtgatgag gtacaggaga ccggccaccg
      601 ccgaggcgat gccctggagt ggttgtccgg cctgctgcca gccgggcagc gaagcgccgc
      661 cgatcaggcc gcagaggccg ttattgagat cgatcgccag ttgcgccagc cagaggctgg
      721 tgttggcgag gagcgcgccg accgccgcgc ggggcaggag ctcggccgcc tcgtgatagg
      781 gcgcgccaat ccggtctttg agcatgacat tgaggccgcc accgacaacg accaccacca
      841 gcagcgcgtt ggccgcgccc cgcaccacgc gccacaggac cgtgacgacg ccgctggcgt
      901 acgagccggc cggtggggtc tggctgacga cgttcaatgg gctggcgagc caggcgtcca
      961 gcggcgcgcg cagggcggca ttgacctcct ggagcgcctc accggccata gtcaagaaga
     1021 ggttcttgat gagctggggg tcgatcgggc tgggtggcag aatcggcggg agcggcaccg
     1081 ccacctcgcg ttgcagcgcg ctcgcgccgc cagcgaggcc gacgccaagg tgctggtcga
     1141 gcaccgcggc gctgctcgtg cgtgccgccg ctacgatgat ggccgccacc ggctgctcgc
     1201 gcggcgccac aggctgcctg aaggcgatgg tggcagcgac cgtgggcgca gcgccgccca
     1261 cgccgagcgc gccaagcgcg atccccagca aacccatgcg gccaagcacc gcgatcagga
     1321 cgtgctgcca cggcgagcgc cggacggcgg gcaagagggt gcgcgccctc gcgtcgcggg
     1381 ggctcatcgt ccgccctcct catcatgcgt cgtccaggtg accagccaca ggtcgggcgg
     1441 gccaccggtc gcggaagcga tcgcgaccag cgcctgaccg tggggcatgt cccagcgcac
     1501 gccggcgaca cggtcggccg ggagggcgag cgtaccgagc gtctgggcgg cagtatcggt
     1561 ggtgagatcg aacgcctgca cggtcagcgg cccgttacgt ttcggccgcg tcagcgcgag
     1621 ggcgaaccca tcgtcgcgcc accctggtcc ccaaccgcca gtgccgggca agcgtcggct
     1681 accgccagcg gtgaggtcgt caacgagcag atcgggtgct ggtgatgcaa acgttggcca
     1741 gacagctgct tgacgccccg gtgcggccgc ggcatagact acgccgcctt ccttggggag
     1801 ccagaccagc ggtgagaagg ccggtggcgc accggcggct ggcacgtcgg ccagcgagcg
     1861 gaagaactgc ccatcgcggc cgaggagaca gagagtggtc cggttgtccg cgcggaccag
     1921 cagcgccagc cggtcgcccg ccgtattcca caggtacgag ccgggaacga cctggccggg
     1981 cagcgtgagg agctctcgcg tggccggtgg cgcggtagcc aggttgagcc actggaggcg
     2041 ctggcgcacg ccgctcccct gcgggtgttg ctcgctgacc agcagcagct gcgtgctatc
     2101 cggcgcccag gcgagatcca ccaggcgctc ggcggcggtg ttcggcggca gcacatagcg
     2161 tcgctgccca ctgtcaccgt tgcggtcggt gacccagacc tcggtcagcc gtccgccggc
     2221 gccgtcgctg gccggtgcgc cgggcgcggc actggcgctt ggctgcgcga gataggcgac
     2281 ctgcgtacca tcgggcgaga ccgccgccgc gccgcgagcg aagggcggtc ccacccgctg
     2341 ggttgcgccg tcctggccga cgatccacac ctggcgctcg tcgcccgccg gcaacgcgag
     2401 ggccagcgcc agccggccat ccgcgagaaa gaccgtcccc gcgatcgtcg cccccggcag
     2461 gggcgagcgg accggccgga cggcaggtgt tcgcaaccac aggataccgg tgaggtcggt
     2521 ggtggcgttg gccgtcaggt gcacggtgta ggtgacgtcg gtacggccag tgtctcgcaa
     2581 ggtgaggcgg tgtgcgccgg gcggcagcgc cagtgtcgct ggcgtctgac cgcgcgactt
     2641 gtcgtcaatt ttgatcgccg cgccgggtgg gttactagcc agcacgagca ttgcgctcgg
     2701 gccaggagac gttgcggccg gctgacgctg ttgcgccagc cggacaacac cgaggacgac
     2761 tggtaggcca atgagcaacc agacccatgg gtgccaccag ccggtcctgc tggggagttt
     2821 gactctgatg atgaatggca gttccatggg tgtcgcctcc cacgtgagcg gctcatgtcg
     2881 agcaccaccg taggaaggct cgctctcaac acgctctcgg ctggctctca tacaagtgat
     2941 ggtggttcag caagatcgcc ctcgcctgac tggccagtga gcaaacaggt gaatggagta
     3001 cccgcctaca gccttaacat atcgtttcgg ttgggagatt catggacaac accagaaaca
     3061 tgcgtggact ggaag
//
LOCUS       DASUBI010000016         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_476725, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000016 DASUBI010000000
VERSION     DASUBI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476725"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFU72_00265"
     CDS             <1..687
                     /locus_tag="VFU72_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5421949.1"
                     /translation="LLHAGRPVPIEALIEWLWPESDPETGANRLYVIVHHLREMIEPS
                     GSGSGRDVIRTSTDHYIFAPAVPCWLDLAEFRAAIAAGHEAEALGDQPKALAAYDTAA
                     QLYRGDLLEDERYAEWCWLEREHLRETHLDALQRLARLAWDAGDLSRAANTYRRALQV
                     DPVREEIQRRLIECLWQLGRRDEALRQYEFLRAVLRRELDITPLPETEELVRRILGAA
                     PQARHSADSV"
     gene            796..1404
                     /locus_tag="VFU72_00270"
     CDS             796..1404
                     /locus_tag="VFU72_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5421950.1"
                     /translation="MGRPAQLRDPRPIQTLFGQTRFAWAWFAVRLFLGWQWLMTGRQL
                     MGDPGWMPGGAELRDFWQRTLHPAGPDSTASPGTWHDLIEFMLRHGWYTWIGPVVAIG
                     AVVAGICLILGLLVGVAAFGGAIVLLQLPPDLSGGANPLALGLAILLMFAWKTAGWIG
                     LDRWVLPLLGAPWRGSTLLGRADQSPTGTPEPDRTPAKRRAG"
     gene            1464..1724
                     /locus_tag="VFU72_00275"
     CDS             1464..1724
                     /locus_tag="VFU72_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421951.1"
                     /translation="MKGGDATTAAESVSREKAPRPSERAADDAHPTVFVITLWLEQGG
                     AAAKPEWRWRVREVRSGEEALFRRVSDVLAYVAARSGGPGPG"
     gene            1776..2606
                     /locus_tag="VFU72_00280"
     CDS             1776..2606
                     /locus_tag="VFU72_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5421952.1"
                     /translation="MKRVARWFALLLALVVLGGLGAAPVLVAAQDPATEGQTVFKEKC
                     AGCHTVGGGKLVGPDLKGVTERRDHDWLVRWLSNPPALVAAGDPTATTLLKENNNVQM
                     PNLGLTGAQIDALLAYLAAPGGASSPAAPALPAGDPARGKAFFVGETRFANGGPPCMA
                     CHSVAGIGALGGGRLGPDLTGAYPKWGQAGLVTFVTQPATVTMNAVWSQTPLTPQEGG
                     DLLAFLQQASVSQRPANTVAQLAVLAACVALLLLVVAQLVWRKRLRGVRRRMVADARR
                     "
     gene            2647..>4664
                     /locus_tag="VFU72_00285"
     CDS             2647..>4664
                     /locus_tag="VFU72_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5421953.1"
                     /translation="MGWIQDLVNPQARRWEEFYRNRWQHDNIIRSTHGVNCTGGCSWA
                     VYVKDGIITWEMQQTDYPQLDPNLPPYEPRGCQRGISASWYVYSPIRVKYPYARGALL
                     DLWRAARAAQPDPVRAWASIVEDEAQRARFHRARGKGGFRRTNWDEVLELIAAACLYT
                     AKRWGPDRVFGFSPIPAMSYLSYAAGSRFLQLFGGVNMSFYDWYADLPNAFPEVWGDQ
                     TDVCESADWYNSKYIISMGSNLNMTRTPDVHFISEARHEGTKFVVIAPDFSQVSKYSD
                     WWIPIEAGQDTALWMAVNHVILKEFHVERAVPYFADYVKRYTDGPFLVTLEPDGANYQ
                     PGRLLRADCVERYAGVEHGDWKFLVYDQLSDQPRMPGGSTGFRWGSQDGKWNLKLQDA
                     LDESPLDPAITMLGREDTVLNVLFHEFAESKAVVRGVPVRYVQTPSGRVPVTTAYDLL
                     MARFGVGRGLPGDYPGSYDEVAAYTPAWQEAYTGIGRDTVIRLAREFAGNAEATEGRT
                     MIIVGASVNHWYYNNLAYRAPITALLLCGCCGRNGGGMNHYVGQEKLSLVAPWTSLAF
                     ALDWSAPPRQQQSPIWHYAHSDQWRYEGEFTEYAAVPPNARWAKGHALDLEAMAVRMG
                     WMPFYPQFNRNPLELAAEATAAGAEDAAAVAQRSIAQLQDGSLDFAVD"
BASE COUNT          710 a         1662 c         1582 g          710 t
ORIGIN      
        1 ctgctgcacg cgggacggcc ggtaccgatc gaagccctga tcgagtggct ctggccggag
       61 agcgatcctg agaccggggc caaccggctc tacgtgatcg tccaccacct gcgcgagatg
      121 atcgagccat caggctccgg cagcggccgg gacgtgatcc gaaccagcac ggatcactac
      181 atcttcgccc cagctgtgcc gtgctggctg gatctggccg agttccgcgc ggccatcgcg
      241 gcggggcacg aggccgaagc gctgggggat cagcccaagg cgctcgcggc ctacgacacc
      301 gcggcccagc tctaccgtgg tgatcttctc gaggacgagc gctatgccga atggtgctgg
      361 ctcgagcgcg agcatctgcg cgagacccat ctggacgcgc tacagcggct ggcccggctg
      421 gcgtgggacg ccggcgacct gagccgcgcc gcgaacacct atcgccgcgc gctgcaggtc
      481 gatcccgtgc gcgaggagat ccaacgccgg ctcatcgagt gtctctggca actggggcgt
      541 cgcgacgagg cgttgcgcca gtacgagttc ttgcgcgccg tcctgcgccg cgagctggat
      601 atcacaccgc ttccggagac cgaagaactc gttcgccgca tcctcggcgc cgcgcctcag
      661 gcacgccatt ccgctgattc cgtgtaaggc tcccgaaagg ttcctcccgt atatcgatgc
      721 cattccggtt gacgcatccg ggccgcgccc gcgttccggc gtaccggccg tcacgctcaa
      781 cgcggaggga accgtatggg acgtccggcg caactgcgcg acccccggcc gattcaaacc
      841 ctcttcggtc agacccgctt cgcctgggcg tggttcgccg tccggctctt tctgggctgg
      901 cagtggctca tgaccggccg gcaattgatg ggggatcccg gctggatgcc gggcggagcg
      961 gagctgcgcg acttctggca gcgcacgctc cacccggccg gcccggattc gacggcctcc
     1021 ccaggcacct ggcacgacct gatcgagttc atgctgcgac acggctggta cacctggatc
     1081 gggccggtcg tcgccatcgg ggcggtcgtg gccgggattt gcctgatcct cggcctgctc
     1141 gtcggcgtgg ccgccttcgg cggcgcgatt gtgcttctgc aactcccgcc cgacctctcc
     1201 ggcggcgcca acccgctcgc cctcggcctc gccatcttgc tgatgttcgc ctggaagacc
     1261 gccggctgga tcggcctcga tcgctgggtg ctgccgctcc tcggcgcgcc ctggcgcggc
     1321 agcaccctgc tcggccgcgc cgatcaatcg ccaaccggta cgcccgagcc ggatcgcacg
     1381 ccggccaagc gccgggcagg gtgacgtgat ggtgcctccc tgacgacccg cgcgcacaga
     1441 accgggcgcc gcgtgtaagc gcgatgaaag gtggcgatgc gacaacagca gcagagtcgg
     1501 tcagccgcga aaaggcgcca cgcccgtccg agcgggccgc ggatgacgcg cacccgacgg
     1561 tgttcgtgat caccctctgg ctggaacagg gaggcgcggc ggccaagccg gagtggcgct
     1621 ggcgggtcag agaggttcgc agcggcgaag aagcgctgtt ccggcgcgtg tccgatgtgc
     1681 tggcctatgt cgcggcgcgc agcggtggcc cgggaccggg ctgaggatgg gaatcgtggg
     1741 gcgggtgagg tgcgtctatg gtgggtagat cccgtatgaa gcgagtggcc cgctggttcg
     1801 cgctgctgct cgcgctcgtg gtcctcggcg gcctcggcgc ggctccggtc ctggtggcgg
     1861 cacaagaccc cgcgaccgag gggcagacgg tcttcaagga gaaatgcgcc ggctgccaca
     1921 cggtcggcgg gggcaagctc gtcgggcccg atctcaaggg cgtgaccgag cggcgcgatc
     1981 acgactggct ggtgcgctgg ctcagcaacc cgcccgccct cgtcgcggcc ggcgatccga
     2041 cggcgaccac gctgctcaag gagaacaaca acgtccagat gcccaatctc gggctgaccg
     2101 gcgcgcagat cgacgcgctg ctcgcctacc tggccgcgcc cggcggggcg agcagcccgg
     2161 cggcgccggc gctcccggcc ggtgatccgg cgcggggcaa ggcgttcttc gtcggtgaga
     2221 cgcgcttcgc gaacggcggg ccgccctgca tggcctgcca cagcgtcgcc ggcatcggcg
     2281 ccctgggcgg cggccggctc gggccggatc tgaccggcgc ctatcccaag tggggccagg
     2341 ccggtctggt caccttcgtc acccaacccg cgacggtcac catgaacgcg gtctggagcc
     2401 agacgccgct taccccgcag gagggcggcg acctgctcgc gttcctgcag caggcgtcgg
     2461 tgagccagcg gccggccaac acggtcgccc agctcgcggt gctggcggcc tgtgtcgcgc
     2521 tgctgctgct cgtggttgcc caactggtgt ggcgcaagcg cctgcggggc gtgcggcgcc
     2581 ggatggtcgc cgacgctcgg cgctgaggcc gaacgccgtg gtattgcgcg tggaaggtca
     2641 aaaacgatgg gctggattca ggatctcgtg aatccccagg cgcgccgctg ggaggagttc
     2701 taccgcaacc gctggcagca cgacaacatc atccgcagca cgcacggcgt caactgcacc
     2761 ggcggctgct cctgggccgt ctacgtcaag gacggcatca tcacctggga gatgcagcag
     2821 accgactatc ctcagctcga ccccaacctc cctccctacg agccgcgcgg ctgccagcgg
     2881 ggcatctccg cctcctggta tgtctacagc ccgatccgcg tgaaataccc ctacgcgcgc
     2941 ggcgccctgc tcgacctctg gcgagcggcg cgcgccgcgc agcccgaccc ggtgcgcgcc
     3001 tgggcctcga tcgtcgaaga cgaggcccag cgtgcgcgct tccaccgcgc gcgcggcaaa
     3061 ggcggcttcc gccgcaccaa ctgggacgag gtgctggagc tcatcgccgc ggcctgcctc
     3121 tatacggcca agcgctgggg ccccgaccgt gtcttcggct tctcgccgat ccccgccatg
     3181 tcgtacctct cctatgccgc cgggtcgcgc ttcctgcagc tcttcggcgg cgtcaacatg
     3241 agcttctacg actggtacgc cgatctcccc aacgcgttcc ccgaagtctg gggcgatcag
     3301 accgacgtct gcgagagcgc cgattggtac aacagcaaat acatcatctc catgggctcc
     3361 aacctgaaca tgacccgcac ccccgacgtc cactttatct ccgaggcacg gcacgagggc
     3421 acgaagttcg tcgtgatcgc gcccgacttc agccaggtgt ccaagtattc ggactggtgg
     3481 atcccgatcg aggccgggca agacaccgcc ctctggatgg ccgtcaacca cgtcatcctg
     3541 aaggagttcc acgtcgagcg cgcggtgccc tacttcgcgg attacgtcaa gcgctacacg
     3601 gatggcccgt tcctggtgac actggagccg gacggggcca actaccagcc gggccgcctg
     3661 ctgcgggccg actgcgtcga gcgctacgcc ggagtggagc acggcgactg gaagttcctg
     3721 gtctacgacc aactgagcga ccagccgcgc atgccgggcg gctcgaccgg tttccgctgg
     3781 ggcagccagg acggcaagtg gaacctgaaa ttgcaggacg ctctcgacga gagtccgctc
     3841 gaccccgcga tcaccatgct cgggcgcgaa gacacggtgc tcaatgtcct tttccacgag
     3901 tttgccgaga gcaaggcggt tgtgcggggc gtgccggtcc gctacgtcca gacccccagt
     3961 ggccgcgtgc cggtcacgac cgcctacgac ctgctgatgg cgcgcttcgg cgtcgggcgc
     4021 gggctgccgg gcgactatcc gggcagctac gacgaggtcg ccgcctacac acccgcctgg
     4081 caggaggcct acaccggcat cggacgcgac accgtcatcc ggctggcacg ggagttcgcc
     4141 ggcaacgccg aggcgaccga ggggcgcacg atgatcatcg tcggcgccag cgtcaaccac
     4201 tggtactaca acaacctggc ctatcgcgcg ccgatcaccg cgctgctcct ctgcggctgc
     4261 tgcggccgca acggcggcgg catgaaccac tacgtcggcc aggagaagct ctcgctggtc
     4321 gcgccctgga ccagcctggc cttcgcgctc gactggtccg cgccgccccg ccagcagcag
     4381 agcccgatct ggcactacgc gcacagcgat cagtggcgct atgagggcga gttcaccgag
     4441 tacgccgcgg taccgcccaa tgcccgctgg gccaagggcc acgcgctcga cctggaggcc
     4501 atggccgtcc gcatgggctg gatgcccttc tatccccagt tcaaccgcaa cccgctggag
     4561 ctcgccgccg aagccacggc cgcgggggcg gaggacgccg cggccgtggc ccagcgctcg
     4621 atcgcgcagc tccaggacgg cagcctcgat ttcgccgtcg acga
//
LOCUS       DASUBI010000017         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1130865, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000017 DASUBI010000000
VERSION     DASUBI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130865"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VFU72_00290"
     CDS             <1..1258
                     /locus_tag="VFU72_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5421954.1"
                     /translation="AIDRLASLALVLEVALGLRVAAADVVQWLTQRRGTLCLFPDADI
                     YWHLAGTIRRGEPFQVVLWGDIPHFALRTPGYPLFLAFCQSVFGESTLAARLVQALIG
                     AGSVWLVFRLTRALVREPEPASRWTTPLLAAALAAIDPYYTGTSALLLSEAVFIPLLL
                     LSLWGLAVLWGQGESATPRARFGWALVVGAASGLAVLVRPSWALGVPALLAAWVVGSG
                     LGRRALIGALVVALAAALVMGPWWLRNERVFGRFVPTALWMGASLYDGLNPKATGASN
                     MDFLNEPDIWPLGEEGQDAILRERALAFARAHPRRVLALAGIKVLRYWSPWPSASEVR
                     SPVLAVLCGAWSVPLYALWLAGLWDRRRDARALVLLAGPLVYFCLLHMVFAGSMRYRI
                     PGEVPALGLAAIGLRRVCGVRETSRI"
     gene            1320..>2038
                     /locus_tag="VFU72_00295"
     CDS             1320..>2038
                     /locus_tag="VFU72_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421955.1"
                     /translation="MRLRVRLGKLLFWTVLLAFCTLAGALGFAYSYYTDSATLRALIK
                     AHIPRYLNATQVDVGTTRVRPLIGDVTITHIIVRQVIDGANFQALVLPWLHVRVDPRA
                     VLEGRFEPQEVVLAHPTLSLCQRRDGTWNFEGLLADPWPAPALKEMPPIVVQNGTIVL
                     TEKLGADQPATISVLREASLRIEPAGGRRLKFEGSAKGEVLDYVKLAGTFDLDTGRVA
                     LSGDVTRIVLSDKLFQHLPGRV"
BASE COUNT          239 a          707 c          746 g          346 t
ORIGIN      
        1 cgcgatcgac cgcctggcgt cgctggcctt ggtgctggaa gtcgcgctgg ggctgcgggt
       61 cgcggccgcc gacgtggtac agtggctcac ccagcgccga ggaacgctgt gcctctttcc
      121 cgacgccgac atctactggc acctcgccgg caccatccgc cggggcgagc cgttccaggt
      181 cgtcctctgg ggcgacatcc cgcacttcgc gcttcgcacg ccgggttacc cgctgttcct
      241 cgccttctgc cagtcggtgt tcggcgagag cacgctcgcc gcccgcttgg tccaggccct
      301 catcggcgcg gggagcgtgt ggctggtgtt ccggctgacc cgggcgctcg tgcgcgagcc
      361 ggagccggcc agccgctgga ccacgccgct gctggcggcc gcgctcgcgg cgatcgatcc
      421 gtactacacc ggcacgtcgg cgctgctgct gtcggaagcg gtcttcattc cgttgctgct
      481 actgtcgctc tgggggctgg cggtgctctg gggccagggc gagagcgcga caccgcgcgc
      541 ccggttcggg tgggccttgg tcgtgggggc ggcgtcgggg ctggcggtgc tggtgcggcc
      601 gtcgtgggcg ctcggtgtgc cggccctgct ggcggcgtgg gtcgtcggct ccgggctggg
      661 gcgacgggcg ctgatcgggg cgctggtcgt cgcgctcgcg gcggcgctgg tcatggggcc
      721 gtggtggctg cgcaacgagc gcgtcttcgg ccggttcgtg ccgacggcgc tctggatggg
      781 cgcgagcctg tacgacggcc tgaaccccaa ggccaccggc gcgagcaaca tggacttcct
      841 caacgagccc gacatctggc cgctcggcga ggagggtcag gacgccattc tccgcgagcg
      901 cgcgctcgcc ttcgcgcgtg cccatccgcg gcgggtgctg gcgctggccg gtatcaaggt
      961 gctgcgttac tggagcccct ggcccagcgc cagcgaggtg cgctcgcccg tgctggcggt
     1021 gctgtgcggc gcgtggagcg tgcccctgta cgccctgtgg ctcgccggcc tgtgggaccg
     1081 ccggcgcgac gcgcgggcac tcgtcctgct ggccgggccc ctggtctatt tctgtctcct
     1141 gcacatggtc ttcgccggat cgatgcgcta ccggatcccc ggcgaagtgc cggcgctggg
     1201 gctggcggcg atcgggctgc ggcgcgtttg cggggtgcgt gaaacgagcc gcatttgaac
     1261 gatcggggat cgtagattcg tacggatcgc acgcaactcc acggagggag ggccgagcga
     1321 tgcgcttgcg ggttcggctg ggaaagctgc tgttctggac cgtgctgctg gcgttctgca
     1381 cgctcgcggg ggcgctcggc ttcgcctatt cctactacac cgacagcgcc acgctacgcg
     1441 ccctgatcaa ggcccacatc ccgcgctacc tcaacgccac gcaggtcgac gtcggcacca
     1501 cgcgcgtcag gccgctcatc ggcgacgtga cgatcacgca catcatcgtc cgccaggtga
     1561 tcgacggggc caacttccag gcgctggtgc tgccgtggct gcacgtgcgc gtcgatccgc
     1621 gcgcagtgct cgaagggcgt ttcgaaccgc aggaagtcgt gctcgcgcat ccgacgctct
     1681 cgctctgcca gcgccgggac ggcacctgga acttcgaggg cctgctggcc gacccctggc
     1741 ctgcgccggc gctcaaggag atgccgccga tcgtcgtcca gaacggcacg atcgtgctca
     1801 ccgagaagct gggggcggat caacccgcga caatctccgt gctccgcgag gcgtcgctgc
     1861 gcatcgagcc ggccggcggc cgccggctca agttcgaggg gtcggccaag ggggaagtgc
     1921 tcgactacgt gaagctcgcc ggcacgttcg acctcgacac cggccgcgtc gcgctgagcg
     1981 gtgacgtcac gcggatcgtg ctctccgaca agctgttcca gcacctcccc ggacgcgt
//
LOCUS       DASUBI010000018         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_681006, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000018 DASUBI010000000
VERSION     DASUBI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681006"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFU72_00300"
     CDS             complement(<1..484)
                     /locus_tag="VFU72_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421956.1"
                     /translation="MDEPASAHAVARGGRLTARRVATLTWAVCLVIALATLILLAIGP
                     GRVLPSDIFSGVGGVSFLVLAMTFASVGALLARRVPENKIGWIFLLTGLANSVQLLSW
                     QYADVGLHATDRLPAVTAAAVFNSLIGEATAGLLGLSLLFFPDGRLPSRRWRPALVSV
                     L"
     gene            652..1302
                     /locus_tag="VFU72_00305"
     CDS             652..1302
                     /locus_tag="VFU72_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421957.1"
                     /translation="MARRDTKSLRTMGFSCIVGAAVLTALAIATQVVQASTDVSKDQW
                     SYPWSSGTSITFDLLGAGSELLLALGVLGLLRSRVAGTARAASLAIWVGFVGTALVVV
                     GHLSSIPVRNELVDDGWPRIVSGVFGLGTVLTAVGFLLAGRATIRAGIWRDWRRFTPL
                     AVGVWSAVLIGLQFTPALPTAVAVYGLFFLALGIALCRQAAPGVEKSALAVQEQAA"
     gene            1299..1751
                     /locus_tag="VFU72_00310"
     CDS             1299..1751
                     /locus_tag="VFU72_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421958.1"
                     /translation="MSEAPTPIELAPKRWSRASGALRGVRAGGRWAHLFLALAVVAGV
                     FVQVYLIGSYIFGAGQGALDAHKTAGWTVHGFELLVFVAALVAWLPRADIVLSLLLAV
                     IGTVQVALAKEHRWVGGLHPLLALVLVGLAATLVVRAMRRRRATGLLV"
     gene            complement(1869..>2129)
                     /locus_tag="VFU72_00315"
     CDS             complement(1869..>2129)
                     /locus_tag="VFU72_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421959.1"
                     /translation="REIPRSRCSIEEARDDPAGSDVPALVCRVDILGSEDFFEEALDE
                     GLVLVLRRVIELIFLEPSAGMRERPRIVLRRERRGQSPPALL"
BASE COUNT          333 a          728 c          724 g          344 t
ORIGIN      
        1 cgaggacgct cacgagcgcc ggccgccagc gccgcgacgg gagccgcccg tctgggaaga
       61 agagaagcga gagaccgagc agtcccgcag tcgcctcgcc gatcaggcta ttgaacacgg
      121 ccgccgcggt cacggccggc aggcggtcgg tggcgtgcag gccgacatcg gcgtattgcc
      181 agctgagcag ctggacactg ttggcgaggc cggtgagcag gaagatccag ccgatcttgt
      241 tctcgggaac ccgccgagcc aggagcgcgc caacggaggc gaacgtcatg gcgagtacca
      301 ggaacgagac cccgccgacc ccggaaaaga tgtcgctcgg aagcacgcgg cccggaccga
      361 tcgccagcaa gatgagcgtc gcaagcgcga tcacgaggca gacggcccag gtgagcgtcg
      421 cgacccgacg cgctgtcagc cggccaccac gtgccacggc atgcgccgac gcaggctcgt
      481 ccacgccgtc attgtctcct ccgccgcgcg ccctgaccag cgcgccggca ctgccgcgca
      541 aatacgcact agcgctgagg catgtcacgc gctgccgcag gtgacgcggc gactccgcgt
      601 tcttagcgtg gcagccaaga gatcagccca aatgacacgg aggagggcga catggcaaga
      661 cgcgacacga agtccctgcg tactatgggt ttcagctgca ttgtcggcgc ggcagtgctc
      721 accgcgcttg ccatcgcgac gcaggtcgtg caggcatcca ccgacgtctc caaggaccag
      781 tggagctacc cctggtcgag tggaacctca atcaccttcg atctccttgg cgccggcagt
      841 gagctgctcc tcgcactcgg cgtgctcggg ctccttcgta gccgtgtcgc cgggacggca
      901 cgcgcggcca gcctcgccat ctgggtcggg ttcgttggaa cggcgctggt cgtggtcggg
      961 cacctgtctt cgatcccggt ccgcaacgag ctcgtcgacg acggctggcc acggatcgtc
     1021 agcggcgtct tcggactcgg aaccgtgctc acggccgtcg ggttcctgct cgccggcagg
     1081 gccacgattc gagccggcat ctggcgcgac tggcgccgct tcacccctct ggccgtgggc
     1141 gtctggtcgg ccgtcctgat cggcctgcag ttcacgccag ccctgccgac cgcggtcgct
     1201 gtctacggac tcttcttcct tgcgctgggc atcgcgctct gcaggcaggc cgcccccggc
     1261 gtggaaaagt ccgcgctcgc cgttcaggag caagcggcat gagcgaggcg cccaccccga
     1321 tcgagctggc gccgaagcgg tggtcgcgcg catccggcgc gctccgcgga gtccgcgccg
     1381 gcgggcggtg ggcgcaccta ttcctcgcgc tcgcggtcgt cgccggcgtg ttcgtccagg
     1441 tctacctgat cggctcctac atcttcggcg cgggccaggg cgcactcgac gcccacaaga
     1501 ccgcaggctg gacggtccac ggattcgagc tgctggtgtt cgtcgccgca ctcgtcgcct
     1561 ggcttccgcg ggccgacatc gtcctgtcgt tgctgctcgc ggtgattgga acggtgcagg
     1621 tagctctcgc aaaagagcac cgttgggtcg gcggcctgca cccgcttctc gcactcgtcc
     1681 tggtaggact ggcggcaacg ctcgtcgtgc gagcaatgcg gcggcggcgc gccacaggtc
     1741 tcctggtgtg aagccgatgc gctcgaagtg ggcgctgctc gcggtcggag gcctgctggg
     1801 cggcgttgct ggagtcgttg gagcgacgat ctccgacccg gaggtcggct tcccagcctg
     1861 ggtggatctc acaggagtgc tggtgggctt tgccctcggc gctctcgtcg caggactatt
     1921 cgcggccgct cgcgcatccc ggcgctcggc tcaaggaaga tgagttcgat gaccctccgc
     1981 agcacgagaa cgaggccctc gtcaagcgcc tcttcgaaga agtcttcact gccgagaatg
     2041 tcgacgcggc agacgagagc gggaacatct gaccctgctg gatcatcacg agcctcctcg
     2101 atcgaacaac gggatcgagg aatctcgcg
//
LOCUS       DASUBI010000019         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_163180, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000019 DASUBI010000000
VERSION     DASUBI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163180"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1784)
                     /locus_tag="VFU72_00320"
     CDS             complement(231..1784)
                     /locus_tag="VFU72_00320"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU5421960.1"
                     /translation="MQATMVMKAGDVFVLTDERGEIDEDVLGAGLYFRDTRFLSEFRM
                     SLNGRSLILLDSSTEHNAAGVTQFTNELLSSAHGEILPHTIGVRRLREVERGLRERVE
                     VHNFNDFPVTLELDLVFGADFRDIFDIRGFRPSGRGQLMLPGQEGNDAILRYLARDNT
                     VYRTHLHFNRLPDDAWVDASEEIPPPSGELETILPGFDRLVRRHHARPLAVLTLRFRL
                     ELGPHALDRLEYLLTPTVQPGPGQPLIEPAFAFPVDEPGERPERFTAPATSNDLVNRA
                     LDRSLRDIRTLITPFPDGRLIAAGIPWYSAPFGRDSLIAALQLLLFSPEIAVETLRYL
                     GRQQGTRVNDWTEEEPGKILHEQRFGEMARLGEVPHIPYYGTVDATPLYVILFVELMR
                     WNGDRALFDELYPCVERAIHWIDAFGDSDGDGFVDYGVRSREGLVNQSWKDSDNSLQF
                     PDGTPVIAPIAAVEVQGYVYHAKRGLAEVLERFGAGALSARAAELRRQAEDLRARFEE
                     RFWLPDAGF"
     gene            complement(1938..>2425)
                     /locus_tag="VFU72_00325"
     CDS             complement(1938..>2425)
                     /locus_tag="VFU72_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5421961.1"
                     /translation="CEMDADFSHDPAALPRLADAAERADLVLGSRYVAGGGTENWSVL
                     RRVISQGGSLYSRVILGLPYRDLTGGFKCFRRRVLERIDLDQVDATGYAFQIEMTYRA
                     DQAGFRVIEVPITFHERRAGESKMGPGIMLEALLRVWKLKFDLRRAEARREQAARRSV
                     L"
BASE COUNT          376 a          814 c          874 g          361 t
ORIGIN      
        1 agcgagttat cgtgcggcca gacgctgccg ttgtggtagc tcatcgggtt gtaggtgggc
       61 atgtcggcgc tgagcgtgcg aatgccccag ccgctgtaga gatcgggggc catcaggcgc
      121 tcgatcaccg gctcggcgcg ctccggcgcc acgataccgc accagagaca gtgccccggg
      181 ttctagctga tggccgggat cagggtgccg ctgccgtcga tcgcctggcc ctagaagccg
      241 gcgtcgggga gccagaagcg ctcctcgaag cgcgcgcgca agtcttcggc ctgccggcgc
      301 agctcggctg cccgcgccga cagggcccca gcgccgaagc gctccagtac ctcggccagg
      361 ccgcgcttgg cgtgatagac gtagccctgc acctccacgg ccgcgatcgg ggcgatgacc
      421 ggcgtgccgt cggggaactg cagcgagttg tcgctgtcct tccagctctg gttgaccagc
      481 ccctcgcgcg agcgcacgcc gtagtcgacg aagccgtcgc cgtcgctgtc gccgaaggcg
      541 tcgatccagt ggatggcccg ctcgacacag gggtagagct cgtcgaagag cgcgcggtcg
      601 ccgttccagc gcatcagctc gacgaagagg atgacgtaga gcggggtcgc gtcgaccgtg
      661 ccgtagtagg ggatgtgcgg cacctcgccc agccgcgcca tctcgccgaa gcgctgctcg
      721 tgcagaatct tccccggctc ctcctcggtc cagtcgttga cccgcgtccc ttgctgccgc
      781 ccgaggtagc gcagcgtctc gaccgcgatc tcgggcgaga agaggaggag ctggagcgcg
      841 gcgatcagac tgtcgcggcc gaagggcgcg ctgtaccagg ggatgccggc ggcgatcagg
      901 cgtccgtcgg ggaagggcgt gatcagcgtg cggatatcgc gcaggctgcg gtcgagcgcg
      961 cggttgacca gatcgttgct ggtcgcgggc gcggtgaagc gctccgggcg ctcgcccggc
     1021 tcgtcaacag ggaaggcgaa ggccggctcg atcagcggct ggcccggccc cggttgcacg
     1081 gtcggggtca gcaggtactc gagccgatcc agcgcgtggg gccccagctc cagccggaag
     1141 cgcagcgtga gcaccgccag cggcctggcg tgatggcggc gcaccaggcg gtcgaagccg
     1201 ggcaggatcg tctccagctc gcccgagggc ggcggaatct cctcgctggc gtcgacccag
     1261 gcgtcgtcgg gcaggcggtt gaagtggaga tgcgtacggt agacggtgtt gtcgcgcgcc
     1321 aggtagcgca ggatggcatc gttcccctcc tggccgggca gcatgagttg cccgcggccc
     1381 gatgggcgga aaccgcggat atcgaagatg tcgcgaaaat cagcgccgaa gaccaggtcc
     1441 agctccagcg tgaccgggaa gtcgttgaag ttgtggacct cgacgcgctc gcgcaggcca
     1501 cgctccacct cgcgcaggcg gcgcacaccg atcgtgtgcg gcaggatctc gccgtgcgcc
     1561 gaggagagca gctcattggt gaactgggta acgccggcgg cgttgtgctc ggtcgaggag
     1621 tcgagcaaga tcagggatcg gccgttcaag ctcatacgga actcgctcaa gaagcgtgtg
     1681 tcgcggaagt agagcccggc ccccagtacg tcctcgtcga tctcgccccg ctcgtcggtc
     1741 aggacgaaga catcgcccgc cttcatcacc atggtcgcct gcatcgcgcg gcatccccct
     1801 ctctccggcc gggcgctccc gaccggcgcg aactcgcttg ccggctcgcg cggcggaccg
     1861 gttgatccgc cgccggccgt gattgcggca ggaagttacg tgccacgctt cgcgcgccgg
     1921 cgcgcgggca ggggagtcta gaggaccgag cggcgcgcgg cctgctcccg gcgtgcctcg
     1981 gcgcggcgca ggtcgaactt cagcttccag acgcgcagca gcgcctccag catgatgcct
     2041 gggcccatct tcgactcacc ggcccggcgc tcgtggaacg tgatcggaac ctcgatgacg
     2101 cggaaaccgg cctggtccgc ccggtaggtc atctcgatct gaaaggcgta gccggtggcg
     2161 tcgacctggt cgagatcgat ccgctccagc acgcggcggc gaaagcactt gaagccgccg
     2221 gtcaggtcgc ggtagggcag gccgaggatc acgcggctat agagcgagcc gccctggctg
     2281 atgacccggc gcaacaccga ccagttctcg gtgccgccgc cggccacgta gcgcgagccg
     2341 agcaccagat cggcgcgctc cgccgcgtcg gccagccgcg gtagcgcggc agggtcgtgc
     2401 gagaaatccg cgtccatctc gcaga
//
LOCUS       DASUBI010000020         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1089918, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000020 DASUBI010000000
VERSION     DASUBI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089918"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..647)
                     /locus_tag="VFU72_00330"
     CDS             complement(33..647)
                     /locus_tag="VFU72_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5421962.1"
                     /translation="MCPATHDEIVTAQPADAWKLSTVVAAAFHPLAVCRWLIPHAIQR
                     RVALPAYFRILVEHALTVGRIDTTPDRTAAALWLPVGADGPEPPEHYERRLKEAVGPH
                     LERFEILDEQFEAHHPLGAEHQHLAILAVHPDHQRQGLGRALLEHRHRQLDQSGSPAY
                     LEASDETNRAWYARYGYRDLGSPIRLPGGPEMFPMMRQPVARNE"
     gene            complement(647..2152)
                     /locus_tag="VFU72_00335"
     CDS             complement(647..2152)
                     /locus_tag="VFU72_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5421963.1"
                     /translation="MRPPPDKDDAVSAALARGRLGVTLVTFFVLASMGPLLVTAGLVP
                     QAYATASLTGVPAAFLAVALVLAVFSVGYVAMARRIANTGAFYAYIARSLGRPLGVGA
                     ALVALVAYNAMQVALYGALGATAQSYAADNFSWHHPWWVYALAFWLVIVVLGLAQVRL
                     SSLLLGVLSLCEILVILVLCVRGLAHPAAGRITFSALAPTSLKLGPTLGALVAIAVLA
                     FVGFEQAAIYTEEARDRERTVPRATFLALVGLAIVYAVASWAMDVHYGAHTVATAQNQ
                     GSLMLFAMAPGIIGSTGRTLFLTALFAAALAFHNAVWRYMFSLGREHVLPARLAKTTA
                     GIPRAASLAQSLLALSVISLAVVFHWDPMGQLFYWAGTAGGYGILLLLALCSLAVIVF
                     FLRDPDGASLWQRWIAPGVATAALAFMAYLVTRNFAALLGVPGGSPSAIWLPALFPLA
                     FLVGLGWALILRLSNREVYDAIGQGRHTASTATQDAPASLPLPAATAEGDL"
     gene            complement(2208..>2520)
                     /locus_tag="VFU72_00340"
     CDS             complement(2208..>2520)
                     /locus_tag="VFU72_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421964.1"
                     /translation="AHPARLSRSMRYQFADPNGGTKITLGLCTKVMRRQPLDAPNTLL
                     AGLVRATGLEHPGLFEVRDDPSARRYLHESLHVLHPPTLRALLTETRAAIEAAVITHR
                     A"
BASE COUNT          425 a          828 c          936 g          331 t
ORIGIN      
        1 caccactcat gtcggccctc cgatctgtga gctcactcat tacgtgcgac cggctgtcgc
       61 atcatcggaa acatttccgg cccgccgggc agccggatcg gactgccgag gtcgcggtag
      121 ccgtaccggg cgtaccaagc gcgattcgtc tcgtcgctgg cctccaggta ggccggcgat
      181 ccgctctggt cgagttgccg gtggcggtgc tccagcagcg cccggcccag cccctgacgc
      241 tgatggtccg gatgcaccgc gaggatcgcc aggtgctggt gctcggcccc gagagggtgg
      301 tgcgcttcga actgctcgtc gaggatctcg aagcgctcca ggtgtggccc gaccgcttct
      361 ttcagccgcc gctcgtagtg ctccggcggt tcaggcccgt ccgctccgac gggcaaccat
      421 agcgccgcgg cggtgcggtc gggggtggtg tcgatcctgc cgacggtgag ggcgtgctcg
      481 accaagatgc ggaagtacgc gggcagggcg acacgccgct ggatcgcgtg cgggatcagc
      541 caccggcaga ccgccagcgg gtgaaacgcg gcggccacca cagtggacag cttccaggcg
      601 tcggcgggct gcgccgtgac gatctcgtcg tgtgtggccg ggcacatcac aggtctccct
      661 cggcggtggc ggcgggcagg ggcagggacg ctggcgcgtc ctgtgtcgcg gtggacgcag
      721 tgtgacggcc ttggccaatc gcgtcgtaga cctcgcggtt actcaggcgc agaatcaacg
      781 cccagcccag accgaccagg aacgccagcg ggaacagtgc gggcagccag atcgcggacg
      841 gcgatccgcc cggtacgccg agcagggcgg cgaagttgcg ggtgaccagg tatgccatga
      901 acgccagggc ggcggtggcc acgccggggg cgatccaccg ctgccagagc gacgcaccgt
      961 ctgggtcgcg gaggaagaac acgatcactg cgagggagca gagcgcgagc agcagcaaga
     1021 tgccgtagcc gccagcggtg cccgcccagt agaacagctg gcccatcgga tcccagtgga
     1081 agacgaccgc cagggagatc accgagaggg cgagcaggct ctgcgccaga gaggctgcgc
     1141 gggggatccc tgcggtggtc ttggccagtc gggcgggcag cacgtgctca cggccgaggg
     1201 agaacatgta gcgccacacg gcgttgtgga acgccagcgc tgcggcgaac aatgccgtga
     1261 gaaacagcgt gcgtccggtg gagccgatga tccccggcgc catggcgaac agcatcagcg
     1321 agccctgatt ctgcgccgtg gcgacggtgt gggcaccgta gtgcacgtcc atcgcccacg
     1381 aggcgacggc gtacacaatc gccagcccga ccaaggcgag gaacgtggcg cgcgggacgg
     1441 tgcgctctcg gtcgcgcgcc tcctcggtgt agatcgccgc ctgctcgaag ccgacgaacg
     1501 cgagcaccgc gatcgcgacc agcgcgccca gcgtcggccc gagtttcagg ctggtcggcg
     1561 cgagcgcgct gaaggtgatc cgcccggccg ccgggtgcgc caggccccgt acacacagga
     1621 cgaggatgac aaggatttcg cagagcgaga gcaccccgag cagcaggctg gacaggcgca
     1681 cctgcgccag cccgagcacg acgatcacca gccagaacgc gagcgcgtag acccaccagg
     1741 ggtggtgcca gctgaagttg tcggcggcgt agctttgcgc ggtggcgccc agcgcgccgt
     1801 agagcgcgac ctgcatcgcg ttgtacgcca ccagcgcgac cagcgcggcg ccgaccccga
     1861 gcgggcggcc gagcgagcgg gcgatgtagg cgtagaacgc gccggtgttc gcgatgcgcc
     1921 gggccatcgc cacatagccg acgctgaaga ccgccagcac gagcgccacc gcgaggaacg
     1981 cggccgggac cccggtcagc gacgcggtgg cgtacgcctg cggcaccagg ccggcggtca
     2041 ccagcagcgg ccccatgctg gccagcacga agaaggtcac cagggtcacg ccgaggcggc
     2101 cgcgcgcaag cgccgctgag accgcgtcgt ccttatctgg tggtggtctc atcagggaca
     2161 gctccttgag ggaagtcggg aatcagagga ttggtgcaga ccggaggtca ggcgcgatgg
     2221 gtgatcacgg cagcttcgat cgccgcgcgg gtttcggtga gcagtgcgcg cagcgtgggc
     2281 gggtgcagca cgtgcaggga ctcgtgcagg tagcggcggg ccgacgggtc atcgcgaacc
     2341 tcgaacaggc ccgggtgctc caggcccgtc gcccggacca gcccggccag cagcgtgtta
     2401 ggcgcatcaa gcggctggcg gcgcatcacc ttggtgcaca acccgagcgt gatcttcgtc
     2461 ccgccgttgg ggtcagcgaa ctggtagcgc atggagcggc taagccgcgc cgggtgggcg
//
LOCUS       DASUBI010000021         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_571649, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000021 DASUBI010000000
VERSION     DASUBI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571649"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFU72_00345"
     CDS             complement(<1..445)
                     /locus_tag="VFU72_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU5421965.1"
                     /translation="MAIISIVGTGYVGLVTGAAFADLGNRVYGIDIDAEKIERLNRGD
                     IPIYEPGLEELVMRNHRAGRLSFTSSYDEAIPESEFVFICVNTPSSFDGDADMRAVRI
                     ASEMISRSMRGHTIVVNKSTMPIGSGDLVSAILDQTRAKGTSFAVV"
     gene            complement(619..894)
                     /locus_tag="VFU72_00350"
     CDS             complement(619..894)
                     /locus_tag="VFU72_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5421966.1"
                     /translation="MEQRTCLVRGRVQGVGFRAFVLQTAAALGLRGWVRNEDDGRSVI
                     VLAQGSTDALDTLEERLRVGPPGALVEHLECRRQRAAGLGAGFVVRR"
     gene            complement(912..2141)
                     /gene="tgt"
                     /locus_tag="VFU72_00355"
     CDS             complement(912..2141)
                     /gene="tgt"
                     /locus_tag="VFU72_00355"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5421967.1"
                     /translation="MMTRDGAPVNGATERDTFQLIRRDTTTAARLGSLRTRRGEVPTP
                     AFMPVGTQASVKALTPEEVATTGTRIVLANTYHLMLRPGPEILREAGGLHAFMRWDGP
                     ILTDSGGFQIFSLAHRREVGEEGVVFRSHLDGSKRQLSPESVIDLQLAFGSDVTMPLD
                     ELVGWDSSHAAQADAMRRTHRWLRRALDHFVTATDGSDNPPLLFGIAQGGFDRDRRGE
                     SAAYVASLPVDGCAVGGLSVGEPKEVMAEMLAASVAALPAEKPRYLMGVGSPEDLWHG
                     VAAGIDLFDCVHPTRVARRGALFSDSGRVNINNARFRRQFEPFDPSCDCLTCRGYSAA
                     YLHHLFRTRELLAYRLATIHNLRFIQRQMATIRAALAAGNFTEAMDTFLDGYRAADLE
                     AAAAQRRARRARRVSDG"
     gene            2163..2810
                     /locus_tag="VFU72_00360"
     CDS             2163..2810
                     /locus_tag="VFU72_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5421968.1"
                     /translation="MRESQSVRPGRRQRALRAAFHLLYRFGGRGYDTLVQALFGSSWD
                     EWRAATVAGLDAEPVLDLGCGTGVLLARMRRQGIDALGIDREPSMLAAARRRGARPLV
                     RAEARALPLRSSSIGGCVATFPAPFILERAVLDEVARVLRPGGRFVVLLGGLPARPSS
                     VPVRFLLRVVYGDQPDIPLPGQSLLCHELLPGAWRRIESPAGPAVVWMSRRRASD"
BASE COUNT          457 a         1024 c         1027 g          546 t
ORIGIN      
        1 agacgaccgc gaacgatgtg cccttggctc tggtctggtc gaggatcgcg ctgaccaggt
       61 cgccggagcc gatcggcatg gtgctcttgt tgacgacgat ggtgtggccg cgcatcgagc
      121 ggctgatcat ctccgaggca atccgcacgg cgcgcatatc ggcgtcgccg tcgaacgacg
      181 acggggtgtt gacgcagata aagacgaact cggactccgg aatggcctcg tcgtagctgg
      241 aggtgaagct gaggcggcca gcgcggtgat tgcgcatcac gagttcctcg agccccggtt
      301 cgtagatcgg gatatcaccg cgattcaggc gctcgatctt ctccgcgtcg atgtcgatgc
      361 catagacccg gttgcccaga tcggcgaagg ccgctccggt caccaggccg acgtaaccgg
      421 taccgacgat cgagatgatg gccatgcgag acgttccctt ccgctcttcc caccggcact
      481 atacgcgcgg cacgtcgccc gcgctcaccc ctactgtacc ggccaatatg ggccatctgg
      541 cagggtcgca ggtactgctt ccctgggtac gatattctac tgtgattacc gtatgcagcg
      601 gcgtgacgaa tcggttgatc aacgcctgac aacgaagccg gctcccaacc cggccgcacg
      661 ctgcctgcga cactcgagat gctccacgag cgcacctggc gggccaacgc gcagccgctc
      721 ttccagcgtg tcgagagcgt ccgtggatcc ctgcgccagc acgataacgc tgcgaccgtc
      781 gtcctcattg cgtacccagc cacgcagccc gagggctgcc gcagtctgga ggacgaaggc
      841 ccggaagccg accccctgga cgcgaccgcg caccaggcag gtgcgctgtt ccaccgtccc
      901 acccgtctca ttcatccgtc gctcacccgc cgcgcccgac gcgcgcgccg ttgcgcggcc
      961 gccgcctcaa gatcggccgc gcggtagcca tcgaggaacg tgtccatggc ctcggtgaag
     1021 tttccggcgg cgagggcggc gcggatagtc gccatctgac gttggatgaa gcgcaggttg
     1081 tggatcgtcg ccagccggta ggccagcagc tcgcgcgttc gaaagagatg gtgaaggtag
     1141 gccgccgagt agccgcggca agtcagacag tcgcagctgg ggtcgaacgg ctcgaattgc
     1201 ctccgaaagc gcgcgttgtt gatgttgacg cgcccgctgt cgctgaagag cgctccgcgc
     1261 cgtgccacgc gcgtggggtg gacgcaatcg aaaaggtcga tgccggcggc gacgccgtgc
     1321 cagagatcct cgggagagcc gacgcccatc aggtagcgtg gcttctcggc ggggagcgcc
     1381 gccaccgacg cggccagcat ctcggccatg acttccttcg gctcccctac gctcaggccg
     1441 ccgacggcgc agccgtccac cggcaggctg gcgacatagg ccgcgctctc cccgcgccgg
     1501 tcgcggtcga acccgccctg cgcgatgcca aagagcagcg gagggttgtc gctcccatcc
     1561 gtcgccgtga cgaagtgatc gagcgcgcgc cggagccagc gatgggtgcg ccgcatcgcg
     1621 tccgcttgcg ccgcgtgaga cgagtcccaa ccaaccagct cgtccagggg catggtgacg
     1681 tccgagccaa aggcgagctg taagtcgatg accgactcgg ggctgagctg ccgcttgctg
     1741 ccgtcgaggt gcgagcggaa gacgactccc tcctcaccta cttcgcgccg atgcgccagg
     1801 ctgaagatct ggaagccgcc ggagtcggtc aggatcgggc cgtcccagcg catgaaggcg
     1861 tgcagaccac cggcctcgcg caggatctcg gggccgggcc gcagcatcag gtggtacgtg
     1921 ttggccagca cgatccgcgt gccggtcgtc gccacctcct ccggcgtcag cgccttgacg
     1981 ctggcctgag ttccgaccgg catgaaagcg ggtgttggca cttcaccccg gcgcgtgcgc
     2041 agcgatccga gccgggcggc ggttgtggtg tcgcgacgga tcagctggaa cgtgtcgcgt
     2101 tctgtggctc cgttgacggg cgcgccgtcc ctcgtcatca tgcggggatg atacggagcg
     2161 ggatgcgcga aagtcaaagc gtccgaccgg gaagacggca gcgggcgttg cgggccgcct
     2221 ttcacctgct ctaccgcttc ggcggccgcg gctatgacac gttggtgcag gcgctcttcg
     2281 gctcgtcgtg ggacgagtgg cgcgccgcca ctgtcgccgg gctcgacgct gagccggtgc
     2341 tcgacctcgg ctgcggcacc ggtgtcctgc tggcacggat gcgccgtcag ggcatcgacg
     2401 cgctgggcat cgaccgtgaa ccgtcgatgc tggccgcggc ccggcgccgg ggagcgcggc
     2461 cgctcgtgcg cgcggaggca cgcgcgctgc cgttgcgcag tagctccatc ggcggctgtg
     2521 tcgcgacctt tcccgcgccc ttcatccttg agcgcgccgt gctggatgag gtggcgcgtg
     2581 ttctccgtcc tggtggccga ttcgtcgtcc tgctcggcgg cctcccggcg cggccgtcgt
     2641 ctgtgccagt tcgcttcctc ctgcgcgtgg tctacggaga ccaacccgat atccccttgc
     2701 cgggccagtc gctactgtgt cacgaactcc tccctggagc gtggcggcgg atcgagtctc
     2761 ccgccggtcc agcggtcgtt tggatgtcgc ggcgccgtgc gtccgactaa gctgtgtgtc
     2821 ttggccacaa agatgcaaag gcgttcggtg agcaggcact tcttcgtcgc gttgctcatc
     2881 ctcatactct gaatagttgt cgtgaaagga gtttacggtg aattcgtccg tgacgactga
     2941 gggttcgttc ctcaccgccg tgctgctgct ctttctggcc ttcttctttc ccccggcggt
     3001 ggcgatgctg cgcggatcgc tgcgtcagat cgcgctctcg ctgatcctga cctt
//
LOCUS       DASUBI010000022         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_217162, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000022 DASUBI010000000
VERSION     DASUBI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217162"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2491
                     /locus_tag="VFU72_00365"
     CDS             <1..2491
                     /locus_tag="VFU72_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU5421969.1"
                     /translation="EQAVGVLQAEVEAARARRAEQEEALRAVTRRQAESERSRAAGEA
                     RLALLDGQSAELAAEQARLGAALAERRDSADDLVQRRDIARAAREASEAQLIALTDAR
                     GVPWAASERAHAELERLRAAERAAQRTLAGLEARHDALARLAESGAGLYAGVRAVLDA
                     SRDGALSGVVGTLAGLLRVPGELEAALEAALGAHLQDIVVERWTDAERAIEHLKRRQA
                     GRATFQPLDTVRSLGVGRAGTGPRGAGIRGVAAGLIEFDPRVEPVVAGLLGRVLVAED
                     LQATRAILSSLSSGWSVVTLDGEIARASGTVTGGARERQSGVLARERELRELPERRRA
                     LEAELSVIRRQANELTARSVGLIDELSRLEEQIAAETDAGRRAAGDEERAERLLADEA
                     RLAGREHARLAEIETRLGALQAEQARARTAIAEARVAAERTGAERERLASQLQTEAGS
                     GQDDALAEARAELRALGERKRALHAERERLATRRLAAERQGAARRERLVALVQELAAL
                     EDEQARLNAAREAAAHDSRQLDGRVAAASAALQAAAAAEQAASAALARLAEEGRALER
                     ERDRLALERSRRADELELLLERAARDLDVPDAAPLLGEAEAIVDPAPLEGRIHRLRER
                     LRRIGLVAEDAVEQYELEADRYRFLRRQLDDVRAAADALRGLMNQLEGNMDEALERTF
                     TEVAEAFSTTFTALFGGGKARLVRVSDEDALSGLDIVAQPPGKRPQNLALLSGGERAL
                     TAVALLFAILKVNPAPFCLLDEVDAALDESNVVRVRDELRALAERTQFVVVTHNRATI
                     EGADTLYGITMGDDGVSRTLSLRLPAEPVTG"
     gene            2703..4280
                     /locus_tag="VFU72_00370"
     CDS             2703..4280
                     /locus_tag="VFU72_00370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5421970.1"
                     /translation="MRRGAGLRAGEGTRFYLALAVITLVALWLRLWAIDFGVPMVTHP
                     DEPNILNPALHMIRTHDFNPHWFIYPALIIYIEAGVVALVQALGHVVSLTAGQVVSAE
                     YVGGRLIMAASGIATVPLVGLIARRMASRRRDLAGLVAAGILAVSYLHVKDSHYLKPD
                     IPTAFFTALTLWFTLDALEHDRRRSWLLAAVATGLAAAAKYTGGAVGLVPAIALVLAS
                     GSWRGLLARWRLAALMAGTALLVFLAINPYVLLDLHTFLSPDIGIVYNSIHYSTGHPG
                     AEGNDTWRWYLAYLWRHGIGPTLTPLVAAGFLAECWRLARGDRRALLLLVFPLLYYLD
                     SSRYVVRFDRQLIPILPFLAIIGGRWLGWLPPLRWRPAWAVRAGVAALLLAAFAVPLV
                     QAARLDAETAKPDLRYAARDWIRANLPAGVVIAREWYTPPVDELGDKDIYLRAVYEQS
                     LAWYRQRGVQYLVVSEFMYGRYLTEPEQYPQEAAFYRALFARPALVHLTRGDRYSGPE
                     IWVFRLDDVADLLPPGP"
BASE COUNT          559 a         1539 c         1641 g          601 t
ORIGIN      
        1 ggagcaagcc gtcggcgtct tgcaagccga ggtggaggcg gcgcgtgctc gccgggccga
       61 gcaggaggag gcgttgcggg ccgtgacgcg ccggcaggcc gagtccgagc gctcgcgggc
      121 agccggcgag gcacgtctgg cgctgctgga tgggcagtcg gccgagctgg cggcggagca
      181 ggcgcggctg ggagcggcgc tggcggagcg gagggacagc gccgacgacc tcgtgcagcg
      241 gcgcgacatc gcccgcgcgg cgcgtgaggc gagcgaggcg cagctgatag cgctgaccga
      301 cgcgcgcggc gtaccctggg cggccagcga acgcgcgcac gcggagttgg aacgcctgcg
      361 ggcagccgag cgcgcggctc agcgcacatt ggccggtttg gaggcgcggc acgacgcgct
      421 ggcgcgactg gccgagagcg gcgccgggct ctacgctggg gtcagggcgg tgcttgatgc
      481 cagccgcgat ggcgccttga gcggagtcgt cggcacgctg gccgggctgc tgcgggtgcc
      541 gggcgagctg gaggcggcgc tggaggcggc gctcggtgcc cacctgcaag acatcgtggt
      601 cgagcgctgg accgacgccg agcgcgccat cgagcacctc aagcgccgcc aggccgggcg
      661 cgccaccttc cagccgctcg atacggtacg gagcctgggc gtcgggcgcg ccgggaccgg
      721 tccgcgcggc gcgggcattc gcggcgtggc cgccggtctg atcgagttcg atccgcgggt
      781 cgagccggtc gtggcggggc tgctcgggcg cgtgctggtc gccgaggact tgcaggcgac
      841 tcgtgccatc ctgtcttcgc tctcaagtgg ctggagcgtg gtgacgctgg acggcgagat
      901 cgcccgcgcc agcggcacgg tcaccggcgg agcccgcgag cgccagagcg gcgtgttggc
      961 acgcgagcgc gagctgcgcg agctgccgga gcggcgccgt gcgctggagg cggagttgtc
     1021 cgtgattcgc cggcaggcaa atgagctgac cgcgcgctct gtcggactga tcgacgagct
     1081 gtcacgcctg gaggagcaga tcgccgcgga gacagacgcc gggcggcggg cggccgggga
     1141 cgaggagcgc gccgagcgat tgctggccga cgaggcgcgg ctcgccgggc gcgagcacgc
     1201 gcggttggca gagatcgaaa cccggctggg agcgctccaa gccgagcagg cgcgggcgcg
     1261 gacggcgatc gcggaggcgc gggtggcggc cgagcgaacc ggcgccgagc gcgagcggct
     1321 ggcgtcccag ctccagaccg aggcgggatc cgggcaggac gacgcgctgg ccgaggcacg
     1381 cgccgagctg cgcgccctcg gcgagcggaa gcgcgcgctg cacgccgagc gtgagcggct
     1441 ggccacgcgg cggctggccg ccgagcgtca gggcgcggcc cggcgtgagc ggctggtggc
     1501 gctggttcaa gaactggcgg cgctggagga cgagcaggcg cggttgaacg cggcgcgcga
     1561 ggccgccgcg cacgactcgc gtcagcttga cggacgggta gcagccgcga gcgcggccct
     1621 acaggccgcc gcggccgccg agcaggcggc cagcgcggcg ctggcgcggc tcgccgagga
     1681 gggccgcgcg ctggagcgcg agcgcgaccg cctggcgctg gagcgtagcc gccgcgccga
     1741 cgagctggag ctgctgctgg agcgcgcggc ccgcgacctc gacgtgcccg acgccgcgcc
     1801 gctccttggc gaggcggaag ccatcgtcga cccggcgccg ctggaggggc gcatccaccg
     1861 cctgcgcgag cgtctgcgcc gcatcggcct ggtggccgag gacgcggtcg agcagtacga
     1921 gctggaggcg gatcgctacc gcttcctgcg acgccagctc gacgacgtgc gcgcggcggc
     1981 cgacgcgctg cgcggtctaa tgaaccagct cgaagggaac atggacgagg cgctggagcg
     2041 caccttcacc gaggtggccg aggcgttctc gacgaccttc accgccctct tcggcggcgg
     2101 caaggcgcgg ctggtccgcg tcagcgacga ggatgcccta tccgggctcg acatcgtggc
     2161 ccagccacct ggcaagcgcc cgcagaacct ggcgcttctc tccggcggcg agcgcgccct
     2221 gaccgcggtg gcgctcctct tcgccatcct gaaggtcaac ccggcgccct tctgcctgct
     2281 cgatgaggtg gacgccgcgc tcgacgagtc gaacgtggtg cgcgtgcgcg acgagctgcg
     2341 cgcgctggcg gagcgcaccc agttcgtggt ggtgacgcac aaccgcgcca ccatcgaggg
     2401 cgccgacacg ctctacggca tcaccatggg cgacgacggc gtctcgcgca ccctctcgct
     2461 ccgtctcccg gccgagccgg tgacggggtg agggggctga cgatgatggt tgggcgctcg
     2521 tgaagacgga tccttcgact gcgctcagga tgacagggtt cttcaggggt gtacgacaac
     2581 gattcgcgtc gtccgaaagg ccgcagactg ttgactgtca tcctgagcgc agtcgaagga
     2641 tccgtcttga cgcggcaccc aacgtcgtcg atagccacct cccctctccc gcgcacgtct
     2701 ccgtgcggag aggggccggt ctccgtgcag gtgagggcac gcgcttctac ctcgccctcg
     2761 ccgtcatcac tctggtcgcg ctctggctgc ggctgtgggc catcgacttc ggcgtgccga
     2821 tggtcaccca ccccgacgaa ccgaacatcc tcaaccccgc gctgcacatg atccgcacgc
     2881 acgacttcaa cccgcactgg ttcatctacc cggcgctgat catctacatc gaggccggcg
     2941 tggtggcgct ggtgcaggcg ctgggccacg tcgtcagcct cacggcgggg caggtggtca
     3001 gcgccgagta cgtcggcggg cggctgatca tggccgcctc gggcatcgcg accgtgccgc
     3061 tggtcgggct gatcgcccgg cgcatggcct cccggcggcg cgatctggcc ggactggtcg
     3121 cggccggcat cctggccgtc tcctacctgc acgtcaagga ctcgcactac ctcaagccgg
     3181 acatcccgac cgccttcttc accgcgctga cgctctggtt caccctcgac gcgctggagc
     3241 acgaccggcg gcgctcctgg ctgctggcgg cggtggcgac cggcctggca gcggcggcca
     3301 agtacaccgg cggcgcggtc gggctggtgc cggccatcgc gctggtcctg gcgtccggct
     3361 cctggcgcgg gctgctggcg cgctggcggc tggccgcgct catggccggc accgccctgc
     3421 tcgtcttcct ggccatcaac ccttacgtac tgctggacct gcacacgttt ctctcgccgg
     3481 acatcggcat cgtctataac agcatccact acagcaccgg ccaccccggc gccgagggca
     3541 acgacacctg gcgctggtat ctggcctatc tctggcgcca cggcatcggc ccgacgctga
     3601 cgccgctggt cgcggcgggc ttcctggccg agtgctggcg gctggcgcgc ggcgaccggc
     3661 gcgccctgct cctgctagtc tttccgctgc tctactacct cgacagcagc cgctacgtgg
     3721 tgcgcttcga ccgccagctc atcccgatcc tgcccttcct ggccatcatc ggcgggcgct
     3781 ggctcggctg gctgccgccg ctgcgctggc ggccagcctg ggccgtgcgc gcgggagtgg
     3841 ccgcgctcct gctggcggcg ttcgccgtgc cgctggtgca ggccgcgcgc ctggatgccg
     3901 agacggccaa gcccgacctg cgctacgccg cccgcgactg gatccgcgcc aacctccccg
     3961 ccggcgtggt gatcgcgcgc gagtggtaca cgccgccggt cgacgagctg ggcgacaaag
     4021 acatctacct gcgcgcggtc tacgagcagt cgctggcgtg gtaccgccag cgcggggtgc
     4081 agtacctggt cgtctcggag ttcatgtacg gtcgctacct gaccgagccg gagcagtatc
     4141 cccaggaggc ggccttctac cgcgccctct tcgcccggcc cgcgctcgtc cacctgacgc
     4201 gcggcgatcg ctacagcggc cccgagatct gggtcttccg cctggacgac gtggcggacc
     4261 tgctgccgcc cgggccgtag cgcggctcac gccgtcgctg accgccgtgc ctcacgcact
     4321 gtaggggcag gcctccgtgt
//
LOCUS       DASUBI010000023         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1049106, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000023 DASUBI010000000
VERSION     DASUBI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049106"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFU72_00375"
     CDS             complement(<1..604)
                     /locus_tag="VFU72_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5421971.1"
                     /translation="MPGRPVSEVVQQAADALDKGNYPLAVEACQRVLGQFPEFARAHL
                     LLATAQLEQGELPAAERAFQETLRREPQSATAYSGLSKVAEQRGELETALAYLQAGWV
                     NAPRDEALRERLARLSERLYGPGGRLHLTRAALGTLHAEAGRWSRAAAECAAVLEEHP
                     GRGDVRLRLAEALWRRGNDEQAAAALKRVLDAEPQAVRALL"
     gene            798..1787
                     /locus_tag="VFU72_00380"
     CDS             798..1787
                     /locus_tag="VFU72_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEU5421972.1"
                     /translation="MSSQQPITPAADPLGILGTARRVAEQARAVAIDPAGVARVAGQI
                     ASQEIAPPAWDRTLHWIGAPEATANYILALDALNFSFWGEPRWVVSYQGQRYNGYWAL
                     AAALTRALHAGVPLDDAAFLAGLDAPRLGEILAGEHPIPLLEERAANLREVGRVLLAR
                     YDGLFARAIKASGPSAVGLVELVVRDFPSFRDVARYQGAEVRFYKRAQILVSDLYGAF
                     EGEGLGAFPDIDRLTAFADYKVPQVLEHLGVLVYAPALAARIDRLEELPAGGPEEIEI
                     RAATIWSIELLRQALAARGRQLAAFEIDWALWSLGQQLPGTARPYHRTRTIFY"
     gene            2226..2708
                     /locus_tag="VFU72_00385"
     CDS             2226..2708
                     /locus_tag="VFU72_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421973.1"
                     /translation="MIEPRNYTSEEMLAWCRFSALTLLGSALAYTRLREGTLDSLIDF
                     VGQRLSDVYGGALGIEPAMLGLLLNVQALGGELRSSMINPAEGEAVVSNLPGEQVAAA
                     VAELFDVAVTSADLLALCDISQAELNRLYDIFGQAAAGAGLDYAREPLDGDQRLTLRA
                     "
     gene            2872..>3374
                     /locus_tag="VFU72_00390"
     CDS             2872..>3374
                     /locus_tag="VFU72_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU5421974.1"
                     /translation="MIPIASGAGDGLGDEGCADLLVTTLTGHKGIDAVAFDPKQHAFR
                     IRYDPDLVSLATVERLADQVGVQLGERYQRCVYHMDGVTCRNCGRAIERQLKRDPRII
                     WASANPPSGSLALEYADSRTQLPTISRPVTVPGATVRAQAPPISEEPEGEEEEETETW
                     WDEHGLLV"
BASE COUNT          520 a         1202 c         1154 g          498 t
ORIGIN      
        1 tcaagagcgc ccgcaccgcc tgtggctcgg cgtccagcac gcgcttgagc gccgcggccg
       61 cctgctcgtc gttgccgcgc cgccagagcg cctcggccag ccgcaggcgc acatcaccac
      121 gccccgggtg ctcttcgagc acggcagcgc actcggcggc ggcgcgcgac cagcgcccgg
      181 cctcggcgtg cagcgttccc agggcggcgc gcgtcagatg cagccgcccg cccgggccat
      241 agagccgctc ggacaggcgc gccagccgct cgcgcagggc ctcgtcgcgc ggcgcgttca
      301 cccaaccggc ctggagatag gccagcgccg tctccagctc gccgcgctgc tcggcgacct
      361 tgctcagtcc gctgtaggcc gtggcgctct ggggctctcg ccgtagcgtc tcctgaaagg
      421 cgcgctcggc ggcgggcaat tccccctgct ccaactgcgc cgtcgccagc aggagatggg
      481 cgcgggcgaa ctcgggaaac tgccccagca cgcgctggca cgcctccacc gcgagcggat
      541 aattcccctt gtcgagcgcg tcggcggcct gctgcaccac ttcacttacg ggacggccag
      601 gcatcgctcc ctcgtttctc tggccctgcc ccggccccct gccgggtcga gcgcacacgg
      661 atcgtcggac gggacgtatg ctccaagtat aaaaagcaag gggaagcacg ctcaactccg
      721 tctctagccg attcttccca tctctcgtcc gcaccgggtg cggccggcga gtcgaggcag
      781 ccatcgagag gatgaccatg agcagccaac agccgatcac gccggcggcc gatccgctgg
      841 gaatcctggg cacggctcgc cgggttgcgg agcaggcacg cgccgtggcg atcgacccgg
      901 ccggtgtggc gcgcgtggcc gggcagatcg ccagccagga gatcgcgccg ccggcctggg
      961 accgcacgct gcactggatc ggcgcgcccg aggctaccgc caactacatc ctggcgctcg
     1021 acgcgctcaa cttcagcttc tggggcgagc cgcgctgggt cgtcagctat cagggtcaac
     1081 gctacaacgg ctactgggcg ctggccgcgg cgctgacccg cgcgctgcac gccggcgtgc
     1141 cgctcgacga cgcggccttt ctggccgggc tcgacgcgcc ccgcctgggc gagatcctgg
     1201 ccggcgagca ccccatcccg ctgctggagg agcgcgcggc caacctgcgc gaggtcggcc
     1261 gggtgctgct ggcgcgctac gacggcctct tcgcgcgcgc catcaaggcc tcggggccca
     1321 gcgccgtcgg gctggtcgag ctggtggtgc gcgacttccc ctccttccgc gacgtggctc
     1381 gctaccaggg cgccgaggtg cgcttctaca agcgcgcgca gatcctggtg agcgatctct
     1441 acggcgcctt cgagggagaa gggctgggcg ccttccccga catcgaccgg ctgaccgcct
     1501 tcgccgacta caaggtaccg caggtgctgg agcatctcgg cgtgctggtc tacgcgccgg
     1561 cgctggccgc gcgcatcgac cgcttggagg agctgcccgc cggcggcccc gaggagatcg
     1621 agatccgcgc ggccaccatc tggagtatcg agctgctgcg gcaggcgctg gcggcgcgcg
     1681 gccggcaact ggccgccttc gagatcgact gggcgctctg gtcgctcggc cagcagctcc
     1741 cgggcacggc gcgtccctac caccgcacgc gcacgatctt ctattagcgc cacaacgccc
     1801 ggcgcgatcg tcggttgtgg agctcgttct tcttaattct gagtagatac cgtcgtaggt
     1861 agtaggggct gtggatggtg tggaaaagcg ggcaaacccg caccggcacg cggatctccg
     1921 gcgcggacaa cctgtggagg atccctccac atctccacct tcgccctcgg cgtcgcctcg
     1981 tgacgctcca ggcacatccc gggtcttccc cagaccatgc acggcgagcc gctgcttgtc
     2041 caatgtacgt acacatcttt gcacggcgct atccacatcc ccacaaggtt atccacagcc
     2101 gggagccgct tatccacaat tccgtcatcc ggcagccgcg gcacggatgg cgcgtgccgg
     2161 atggtcaatg gtcaggggaa cggcccgacg ccaaaggatc aagcaccgag gaacaaggag
     2221 tgacgatgat cgagccgaga aactacacga gcgaggaaat gctggcctgg tgccgcttca
     2281 gcgcgctcac gctgctgggc agcgcgctgg cctacacccg gctgcgggag ggcacgctcg
     2341 attcgctgat cgacttcgtc gggcagcgcc tgagcgatgt ctacggcggc gcgctgggga
     2401 tcgagccagc catgctgggg ctgctgctga acgtgcaggc gctcggcggc gagctccgct
     2461 ccagcatgat caacccggcc gagggcgagg ccgtggtctc gaacctgccc ggagagcagg
     2521 tggccgcggc cgtggcggag ctgttcgacg tggccgtcac gagtgcagac ctgctggcgc
     2581 tctgcgacat cagccaggcg gagctgaacc ggctctacga tatcttcggc caggccgcgg
     2641 ccggcgcggg gctggactac gcgcgcgagc cgctcgacgg cgaccagcgc ctgacgctgc
     2701 gggcgtagga gcgtgtgcga ggtgcccatc gacgacgctt ggcggccccg cccgccgcat
     2761 tgactcgcgg gcgcggctgc ggtaccctcc agtagggaaa taggactcat tctcaactag
     2821 ggtcacgcga tgattcagtg aagggctcgg ggctcaggga tgcaggagcg aatgataccg
     2881 atagccagcg gggccggcga tgggctgggc gatgagggct gcgccgatct cctggtcacg
     2941 accctcaccg gacacaaagg gatcgacgcg gtcgcgttcg atccgaagca gcacgccttc
     3001 cgcatccgct acgaccccga cctcgtctcg ctggccaccg tcgagcggct cgccgatcag
     3061 gtcggcgtgc aactcggcga gcgctaccag cgctgtgtct accatatgga cggcgtcacc
     3121 tgccgcaact gcggccgcgc gatcgagcgc caactgaagc gcgatccccg gatcatctgg
     3181 gccagcgcca acccgccgag cggcagcctg gcgctggagt atgccgacag ccgtacccag
     3241 ctccccacga tctcccgccc ggtcacggtc ccgggcgcca cggtacgcgc gcaagcgccc
     3301 ccgatttcgg aggagccaga gggcgaggag gaagaggaga ccgagacctg gtgggacgag
     3361 cacggcctgt tggt
//
LOCUS       DASUBI010000024         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_830639, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000024 DASUBI010000000
VERSION     DASUBI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830639"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFU72_00395"
     CDS             <1..1014
                     /locus_tag="VFU72_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421975.1"
                     /translation="PATEPVMDVAVVTESTLPEVAEAPRETALEPPASSEEATEAPAV
                     EPPAADEQLAAAEPVAKTVEAEEPAAAAEQSAPEEPLVAQEEPAANEHRTVAGEPEAE
                     EGADTGVPEQAVAEAGQQEQEREQELVAVGAAIAAQAQSTEAGEAPTAGPAPEAGNEE
                     LAAEAAVLGSEETAEASAEPTAGPGTPSEEAAPPVTEPSSTRQLFDALSAADQPAEAE
                     LLQQLRAEIAFLRQQMREKDRQIAAWITESRRKDLSLTRLENRLRVVPGEHGRARNGT
                     EAPASTSDLLAAREQLMAPLAQRLHQLEDRLGSLEASRAPRRPAPWYRRLIGSGSDRQ
                     SAS"
     gene            complement(1007..1216)
                     /locus_tag="VFU72_00400"
     CDS             complement(1007..1216)
                     /locus_tag="VFU72_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421976.1"
                     /translation="MTVRGEWNEPVATPGRAERAREGCLQFCCSCLAVAALVIVAGSF
                     IFFVFGMTSVVAHSWEILSHWLLLN"
     gene            complement(1220..>2070)
                     /locus_tag="VFU72_00405"
     CDS             complement(1220..>2070)
                     /locus_tag="VFU72_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU5421977.1"
                     /translation="RAIGGFIQDLTGAPVRLRDADRSYQIELLHEHAYVNSAVLRGPG
                     GLPVGASGRVVALISGGYDSPVAAYRLRKRGCALTLVHCHAYPFVRSTSIEKVIELAQ
                     HLGRHQPGVRLNFVPIGELQRVIALTAPPELRVVLYRRLMLRVAEAIAREEDAGALVT
                     GESLGQVASQTLPNIQAIEAAVDLPVLRPLIGLDKEEILEQARALGTEVISRTPDDDC
                     CTVFVPRFPATHATPAEAAGAEAGFEPRELVRLALERRVTLDGDPRDWNVAQVLAGSA
                     ATLGVA"
BASE COUNT          347 a          722 c          754 g          247 t
ORIGIN      
        1 ccggcgaccg agccggtgat ggacgtggca gtggtcacgg agtccacgct gccggaggtg
       61 gctgaagcgc cccgtgaaac cgctctcgag ccgccggcgt cctcggaaga ggccaccgag
      121 gcgcccgccg tggagccgcc cgcggctgac gagcaactcg ccgccgcgga gccggtggcg
      181 aagaccgtcg aggccgagga accagccgca gccgcggaac aatccgcgcc tgaggagccg
      241 ctcgtcgccc aggaagagcc tgccgcaaac gagcaccgaa ccgtagccgg cgagcctgaa
      301 gcggaggaag gggcagacac cggggttcct gagcaggctg tggccgaagc ggggcagcag
      361 gagcaggagc gggagcagga gttggtcgcg gtcggagcgg caatcgcggc acaggcgcaa
      421 tccaccgaag ccggtgaggc gccgacagca ggcccggcac ctgaggccgg gaacgaggag
      481 ttggccgcgg aggcggcagt tctgggaagc gaggagaccg ctgaagccag cgcagagccg
      541 actgccgggc cgggaacccc gtcggaagaa gcggcgccgc cggtaactga accgtcctcc
      601 actcgccagc tcttcgacgc gctctccgcc gcggaccagc cagccgaggc ggagctgttg
      661 cagcagcttc gcgccgagat cgcgttccta cgccagcaga tgcgcgagaa ggatcgccag
      721 atcgcggcct ggatcaccga gagccggcgc aaggatctca gcctgacacg cctggagaac
      781 cggctgcgcg tcgtgcccgg ggagcatggc cgcgcccgca acggcaccga ggcgccggct
      841 tcgacctccg acctgctggc ggctcgcgag caattgatgg ccccgctcgc ccagcgcctc
      901 caccagctcg aggaccggct gggtagcctg gaagccagcc gggctccgcg gcgacccgcg
      961 ccctggtatc gccgcttgat cggctccggc agcgatcgcc agagcgctag ttgagcagga
     1021 gccagtggct gaggatctcc cagctgtggg cgaccactga ggtcatgccg aagacgaaga
     1081 agatgaaact accggccacg atcaccagcg ccgcgacggc gaggcaactg cagcagaatt
     1141 gcaggcagcc ttcgcgggcg cgctctgccc gccccggggt cgcgaccggc tcgttccact
     1201 cgccacgcac cgtcacgggc taggccactc ccagcgtcgc ggcggagccg gccagcacct
     1261 gagccacgtt ccagtcgcgc ggatcaccgt cgagtgtcac ccggcgctcc agggccagcc
     1321 gcaccagctc ccgcggttcg aaccccgctt cggcaccggc tgcctcggcc ggcgtggcgt
     1381 gggtggcggg gaatcgcggc acgaagacgg tgcagcagtc gtcgtcgggg gtgcgtgaga
     1441 tcacctcggt gccgagcgcg cgcgcctgct ccagaatctc ctctttatcc agcccgatca
     1501 gcgggcgcag gacggggaga tcgacggccg cctcgatcgc ctggatattc ggcagcgtct
     1561 gcgaggccac ttgccccagg ctctcgccgg tgaccagcgc gccggcgtct tcctcgcgcg
     1621 cgatcgcctc ggccacgcgc agcatcagcc gccggtagag caccacgcgc agctccggcg
     1681 gcgcggttag cgcgatgacg cgctgcagtt cgccgatcgg cacgaagttg agccggacgc
     1741 ccggctggtg gcggccaaga tgctgggcca gctcgatgac cttctcgatc gaggtcgagc
     1801 gcacgaaggg gtaggcgtga cagtggacca aggtcagcgc gcaaccgcgc ttgcgcaggc
     1861 ggtaggccgc caccggggag tcgtagccgc cggagatcag ggcgacgacg cgcccgctgg
     1921 cgccgaccgg caggccaccg gggccgcgca gcaccgccga gttgacgtag gcgtgctcgt
     1981 gcagcagctc gatctggtag ctgcggtcgg cgtcgcgcag gcgcaccggg gcgccggtta
     2041 gatcctggat gaagcccccg atcgcgcgct
//
LOCUS       DASUBI010000025         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_912578, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000025 DASUBI010000000
VERSION     DASUBI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912578"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFU72_00410"
     CDS             complement(<1..495)
                     /locus_tag="VFU72_00410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5421978.1"
                     /translation="MGMDEARRAEVGRHFEERYADASERDDTASVPWARLAPNPLLLD
                     WLTEHAVQGEGRPALVIGCGLGDDAEALAQAGFRVTAIDVAPSAVAWCRRRFPASGVD
                     YQTVDLFAAPAGFQHAFDLVFDAYTLQALPEELRSEAMRAIAGFVAPGGRLLVIQRAR
                     QPNEP"
     gene            complement(602..>2191)
                     /locus_tag="VFU72_00415"
     CDS             complement(602..>2191)
                     /locus_tag="VFU72_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5421979.1"
                     /translation="LELANRLEVVSHSVLAVTSALELDTVLRRIVDLARELAGARYAA
                     LGVPGHDGRLQAFVTSGIDEAEAAAIGPPPRGLGILGLLLAEPKTIRLSDLTRHPASV
                     GFPKGHPAMHSFLGVPIVARGRVVGNLYLTEKLRGQDFSEEDAQLVEMLARHAAVAIE
                     NARLYQAVEEQERRLGLLIDQLPEAVLVLATEPERVSLANREASRLLGWQIEPPVPLA
                     DFLSRNPRLHTDGSSLPLEDVHIVRSLREGALVERDEIRLRRTDGAILTLLVNSVPLA
                     APDRGGTIHGAIAVFQDITQIKDAEQLKDDFLSLVSHELRTPLTTIRGDATLLQRDWA
                     RLPAGAREDLLNDLSAESQRLAILIQNMVQLASIRAGRLALEGEPVLVGALIERAAAA
                     IAELAPGQPLTVAVEPGLVAEADPSLVEQTLHILLNNAVKYAPGAPIEVSGRRVEGQI
                     VVAVRDHGPGIEVEELPHMFERFHRARRSRESQTPGLGLGLYLARHLVEANGGRIWIE
                     SPPDGGARIAFSLPAASPDEE"
BASE COUNT          298 a          782 c          813 g          298 t
ORIGIN      
        1 cggctcgttg ggctgacggg cgcgctggat gaccagcagc cggccgccgg gcgcgacgaa
       61 gccggcgatg gcgcgcatgg cttccgaacg cagctcctcc ggcagcgcct gcagggtgta
      121 ggcgtcgaag accaggtcga aggcgtgctg gaagccggcg ggcgccgcga agaggtcgac
      181 ggtctgatag tcgacaccgg aggcggggaa gcggcgacgg caccaggcga ccgccgaggg
      241 cgcgacgtcg atggcggtga cgcgaaagcc ggcctgagcc agcgcctcgg cgtcgtcgcc
      301 caggccgcag ccgatcacca gcgcgggccg gccctctccc tggacggcgt gctccgtcag
      361 ccagtcgagc aagagcgggt tgggggccag ccgcgcccag gggacgctgg cggtgtcgtc
      421 gcgctcgctg gcgtcggcgt agcgctcctc gaagtgccgg ccgacctccg cccgccgcgc
      481 ctcgtccatg cccatgctgc atcccctttc gcgtcgtgct cgccggatga cgggggaccg
      541 gcggcggtcg cccgcgccgt ggcgttaggt gtaccggacg ggcgcgccgg ggacaagcca
      601 ttcactcttc gtcggggctg gcggccggca ggctgaaggc gatgcgcgcg ccgccatcgg
      661 gcggcgattc gatccagatg cggccgccgt tggcctcgac caggtggcgc gccaggtaga
      721 ggccgagccc gaggccgggt gtctggctct cgcggctgcg ccgggcgcgg tggaagcgct
      781 cgaacatgtg cggcagctcc tccacctcga tgcctgggcc gtggtcgcgc acggccacca
      841 cgatctggcc ctcgacgcgt cgcccgctca cctcgatcgg cgcgccgggg gcgtatttca
      901 cggcgttgtt cagcaggata tgcagcgtct gctcgaccag agacggatcc gcctcggcca
      961 ccagccccgg ctcgaccgcg accgtgagcg gctggcccgg cgccagctcg gcgatggccg
     1021 ccgccgcgcg ctcgatcagc gcgccgacca gcaccggctc gccctccagc gccagccgcc
     1081 cggcgcgaat gctggccagc tgcaccatgt tctggatcag gatcgccagc cgctggctct
     1141 cggccgagag gtcgttgagc agatcctcgc gcgcgcccgc cgggagacgc gcccagtcgc
     1201 gctgcaggag cgtggcatcg ccgcggatgg tggtcaacgg ggtgcgcagc tcgtgcgaga
     1261 cgagcgagag gaagtcgtcc ttgagctgct cggcgtcctt gatctgggtg atgtcctgga
     1321 aaacggcgat ggcgccgtgg atcgtgcccc ccctgtccgg cgcggccagc ggcacgctgt
     1381 tgaccagcag cgtcaggatg gcgccgtcgg tgcgccgcag ccggatctca tcacgctcca
     1441 ccagggcgcc ctcgcgcagc gagcgcacga tgtggacgtc ctccagcggc agcgatgagc
     1501 cgtccgtgtg caggcgcggg ttgcgcgaca ggaagtctgc cagcgggaca ggcggctcga
     1561 tctgccagcc gagcaggcgc gaggcctcgc ggttggccag gctgacccgc tccggctcgg
     1621 ttgccagcac cagcacggcc tccggcagtt gatcgatcag cagccccagg cgccgctcct
     1681 gctcctcgac cgcctgatag aggcgcgcgt tctcgatggc gaccgcggcg tggcgcgcca
     1741 gcatctcgac gagctgcgca tcctcctcgc tgaagtcctg cccgcgcagc ttctcggtga
     1801 gatagaggtt gccgacgacg cggccgcgcg ccacgatcgg cacccccagg aagctatgca
     1861 tcgcgggatg gcctttcggg aagccgaccg aggcggggtg ccgggtcagg tcgctgaggc
     1921 ggatcgtctt cggctcggcc agcagcaggc cgaggatccc caggccgcgt gggggcgggc
     1981 cgatggcggc ggcttcggcc tcgtcgatgc ccgaggtgac gaacgcctgc aagcggccgt
     2041 cgtgccccgg cacgcccagc gcggcgtagc gcgcccccgc cagctcgcgc gccaggtcga
     2101 cgatgcggcg cagaaccgta tcgagctcca gcgcgctcgt gaccgccagc acggagtggc
     2161 tgacgacctc caaccggttg gccagctcca g
//
LOCUS       DASUBI010000026         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_217025, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000026 DASUBI010000000
VERSION     DASUBI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217025"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFU72_00420"
     CDS             <1..267
                     /locus_tag="VFU72_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421980.1"
                     /translation="RRERPEPDRDRSSDTMANQTESAEMELLEQNRQRLLRELRSKAR
                     QYEARHELRSTELEAALASGRLHDTAEVCDWVIAVHTIRALERE"
     gene            432..731
                     /locus_tag="VFU72_00425"
     CDS             432..731
                     /locus_tag="VFU72_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421981.1"
                     /translation="MSMRQESYFRGRQRFVRTVEYSYHVLRRAGTEVYNLFRYDNIHV
                     QPGHTSRHHLHRFDDLGHEIEPPLQVGDEGWPHLSDVIEQAYQWTLGHPEGPARE"
     gene            complement(764..1468)
                     /locus_tag="VFU72_00430"
     CDS             complement(764..1468)
                     /locus_tag="VFU72_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421982.1"
                     /translation="MAGGTLVALNNSNWEPVLSRAASYIPADAPALLARVIDERAIEE
                     YGVAARNLLQGPGRVAHFEAAMAEAAERAAREQLAEARQQLLALRPDLAVETLLLRGI
                     PGEALVAAAREQAAGTLIITRDQAEQEQVEHEGVIASWSRNRHGDLNGFTLADGTEVR
                     VPPHRGAALAAALPAGTPVLVSGEPRRAHLHAWSVVNRATGAALEAHAPKEAPLGPDA
                     RFVVDHAPCDVLLLRV"
     gene            complement(1762..>2042)
                     /locus_tag="VFU72_00435"
     CDS             complement(1762..>2042)
                     /locus_tag="VFU72_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEU5421983.1"
                     /translation="PMTIVTAGAVGPLTREGFLRPASAYAALLGTNLGPNLTTVGSLA
                     TLIWLSIVRGRGLTISARDYLRLGLIATPPILASAILGLWLSLKLFGG"
BASE COUNT          339 a          683 c          680 g          340 t
ORIGIN      
        1 cggcgtgagc gacctgagcc cgatcgagat aggagcagcg atacgatggc gaaccagacc
       61 gaatctgcgg aaatggagct gctcgagcag aatcggcaac gcttgcttcg agagctgcgc
      121 tcgaaggcgc gacagtacga agcacgccat gagcttcgtt ccaccgaact cgaggcagcg
      181 ctcgcctcgg gacgcttgca cgacacggct gaagtctgtg attgggtaat cgccgttcac
      241 accattcgcg ctcttgaacg tgagtgaccc ccacggctgg aatagctggg atgccttcct
      301 cgctattcac gacagccacc tgcgtcatta cgaccacttc attctgggtt tgcacgctga
      361 acttcactcc aacgactacg acaatctact ggagcggagt cctctcctgc acgcacaaca
      421 tcgagatcta tgtgagcatg cggcaggagt cctattttcg gggtcgtcaa cggttcgtgc
      481 gcacggttga gtacagctat catgtgttgc gccgtgcggg aaccgaggtc tacaacctgt
      541 tccgctacga taacattcat gttcagcccg gacacaccag tcggcaccat ctccaccgct
      601 tcgacgactt gggccatgag atcgaaccgc cgctccaggt tggtgacgag ggctggccgc
      661 atctatccga cgtgatcgaa caggcctacc agtggacatt gggccatcct gagggtccgg
      721 cgagagaata ggtatcgggc taccagttcg cccgcgtgtc gcactacacc cgcagcagga
      781 gcacatcaca gggggcgtgg tcgacgacga agcgggcgtc ggggccgagc ggggcctctt
      841 tgggggcgtg cgcttcgagg gcggcgccgg tcgcgcggtt gactacgctc caggcgtgca
      901 ggtgcgcgcg gcgcggctcg ccgctcacca gcactggcgt gcccgccggc agcgccgccg
      961 cgagggccgc gccgcggtgc ggcggcacgc gcacctccgt gccgtcggcc agggtgaagc
     1021 cgttgaggtc gccgtggcgg ttgcggctcc agctggcgat caccccctcg tgctctacct
     1081 gctcctgctc ggcctggtcg cgcgtgatga tgagcgtgcc ggccgcctgc tcgcgcgccg
     1141 ccgccaccag cgcctcgccg gggatgccgc gtaagaggag cgtctcgacg gcgaggtccg
     1201 gccgcagggc gagcagttgt tgtcgcgcct cggccagttg ctcccgcgcc gcacgctcgg
     1261 ccgcctccgc catggccgcc tcgaagtggg ccacgcggcc tggcccctgc aacaggttgc
     1321 gcgccgccac gccgtactcc tcgatggcgc gctcgtcgat cacgcgcgcc agcaaggccg
     1381 gcgcgtccgc cgggatgtag cttgcggccc ggctcagcac aggttcccag ttgctattgt
     1441 tgagtgcgac gagcgtgccg cctgccatgg tcgctgcctc cttcgctcgc gcgggccgcg
     1501 ccccgattct agccgctcgg cactgcctga tgaacacggt tggtgccggg tgaggacgga
     1561 tccttccttc ggcaggctca ggatcaagct caggatgaca gacataattt gaggctgccg
     1621 aagacggtca tcgtcgtcca aaaggccacc aaccaaaccc tggcatcctg agcgcaggcg
     1681 aaggatccgt gctggagcgg cacgcagtct cttcgatggc gaccgagtcg atcccgcgct
     1741 gattcggtcc acagaccggg cctagccgcc gaagagcttc agcgagagcc agaggccgag
     1801 gatggcgctg gccaggatcg gcggcgtggc gatcagcccc agtcgcaggt agtcgcgcgc
     1861 cgagatcgtc aggccgcgcc cgcgcacgat gctcagccag atcagcgtcg ccagcgagcc
     1921 gaccgtggtc aggttcggcc cgaggttggt gccgagcagc gcggcgtagg cgctggccgg
     1981 gcgcaggaac ccctcgcgcg tcagcgggcc gaccgcgccc gccgtgacga tcgtcatcgg
     2041 ga
//
LOCUS       DASUBI010000027         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_13548, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000027 DASUBI010000000
VERSION     DASUBI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13548"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..695
                     /gene="groES"
                     /locus_tag="VFU72_00440"
     CDS             405..695
                     /gene="groES"
                     /locus_tag="VFU72_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5421984.1"
                     /translation="MGNLKPLGDRVVVKALEREEVTKSGIYLPDTAKEKPQEGRVEAV
                     GPGRILDNGERSKMELKEGDRVLFAKYAGTEFKLDGEDLLILRESDILAVIS"
     gene            737..2377
                     /gene="groL"
                     /locus_tag="VFU72_00445"
     CDS             737..2377
                     /gene="groL"
                     /locus_tag="VFU72_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477351.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5421985.1"
                     /translation="MAKQIEFNEEARRSLKRGVDTIANAVKTTLGPKGRNVALDRKYG
                     APTVTHDGVTVAKEIELKDPFENMGAQLLKEAATKTNDIAGDGTTTATVLAQAIVNEG
                     LRNVAAGANPMLLKRGIDQGVNALVERIRENAKPVNSQEEIASVATISAADAEIGPLI
                     AEVMEKVGKDGVITVEESRGISFETEYVEGMQLDRGYISSYFVTNADRMEAVLDDPYI
                     LITDKKISALSDLLPVLEKFLQISKNLLIIAEDIDGEALATLVVNRLRGVLNVVGVKA
                     PGFGDRRKEMLQDIAILTGGQVISEDIGRKLDSTTVKDLGRARRVVVTKDETTIIEGK
                     GDEAAIRARVDQIKTLIEQTTSDYDREKLQERLAKLAGGVAVIKVGAATETELKEKKH
                     RVEDALSATRAAVEEGIVPGGGVALLNALPALDSIKADGDIATGVSILRRALEEPMRQ
                     IALNAGQDGAVVVETVRRKATDENNASVGYNVLSGQYGDMVAQGIIDPAKVTRSAVEN
                     AASIAGMILTTEALITDIPEEKPAMPAGGGMPGGGMDY"
     gene            complement(2597..>3697)
                     /locus_tag="VFU72_00450"
     CDS             complement(2597..>3697)
                     /locus_tag="VFU72_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5421986.1"
                     /translation="VNYLNSCSLGALSLRAMAGVNEYLTLWGSMGASAWYEIWLGRLA
                     ALRTNLGALINAGADEIAIGPSISGAVSVIASCLDYSKRNKVVMADLDFPTLGHQWLA
                     KRKQGVEVEFVPSRNRVSVDLEAFERAVDERTALVATSHVFFTSGFVQDIKAIAEIAH
                     RKGALVLIDAYQATGQIPTDVKDADVDFLLSGGLKWLLGGTGIVFTYARRELIPQLEP
                     TIPGWFAVNNQFGFDIHALEYRPDAGRLEQGTPSISAVYALLGGLSYVQEIGPPALRE
                     RTQHLSEDLIARALAAGFVPRVAEDPTQRSGIVLLNMDNPRPVVAALAQAGTIVDMRP
                     GAVRVSPYFYNTIEENQVVIDAMVQIRDQPGS"
BASE COUNT          720 a         1159 c         1228 g          590 t
ORIGIN      
        1 aatagatggc gcgtgctgcg cccggctggt tcctggcacg ggctgggcga ccaatacgct
       61 ggcgataaac gagtacggcg cgataaatcg cgcggctaca caatggctaa tcggtagttg
      121 tgccaaggca ggccgcgcag gcggccttcg taagggtagc gggcggcttg agccgccccg
      181 gcggcgccgc cggcccggat tctattagca ctctaacaaa ctgagtgcta aaattggccc
      241 gcaaaccctt gcaatcggcg gcgttttgct gtatattggg gttagcactc agacgacgag
      301 agtgctaacc ccgtcaaaag tagcgtgcgc cgggaaactg tgcgccgcgt gcggcttatt
      361 gacacatttc caccgaggcc ggtggatgca aggaggttcg ttggatggga aacctgaaac
      421 cgttgggtga tcgcgtggtg gtcaaggcgc tggagcgcga agaagtgacc aagagcggca
      481 tttatctgcc ggatacggcc aaggagaagc cccaggaggg ccgcgtcgag gccgtcggtc
      541 cgggccgcat cctcgataac ggcgagcgct cgaagatgga actcaaggaa ggcgatcgcg
      601 tgctcttcgc gaagtatgcc gggaccgagt tcaagctgga cggcgaggat ctgctgatcc
      661 tgcgcgagag cgatattctg gctgttatca gctaaccttg tattagactc agtttcatca
      721 ggaggattta ggttttatgg ctaagcagat cgaattcaat gaggaagccc ggcgctcgct
      781 gaagcgcggc gtggacacga tcgccaacgc ggtcaagacc accctcggcc ccaaggggcg
      841 caacgtggcc ctcgaccgca agtatggcgc gccgaccgtc acccacgacg gcgtgaccgt
      901 cgccaaggag atcgagctga aggacccctt cgagaacatg ggcgcccaac tgctcaagga
      961 agccgccacc aagaccaacg acatcgccgg tgacggcacc acgaccgcga ccgtgctcgc
     1021 tcaggcgatc gtcaacgagg gtctgcgcaa cgtggccgcc ggcgccaacc ccatgctgct
     1081 caagcgcggc atcgaccagg gtgtgaacgc cctcgtcgag cgcatccgcg agaatgccaa
     1141 gccggtcaac tcgcaggaag agatcgccag cgtcgccacc atctccgccg ccgatgccga
     1201 aatcggcccc ctgatcgccg aggtcatgga gaaggtcggc aaagatggcg tcatcaccgt
     1261 cgaggagagc cgcggcatca gcttcgagac ggaatacgtc gagggcatgc agctcgatcg
     1321 cggctacatc agctcctatt tcgtcaccaa cgcggaccgc atggaagccg tactggacga
     1381 cccctacatc ctgatcaccg acaagaagat ctccgccctc agcgacctgc tgcccgtgct
     1441 ggagaagttc ctccagatca gcaagaacct gctgatcatc gccgaggaca tcgacggcga
     1501 ggccctcgcc accctggtgg tgaaccgcct gcgcggcgtg ctcaacgtcg tcggcgtgaa
     1561 ggcccccggc tttggcgacc gccgcaagga gatgctccag gacatcgcga tcctgaccgg
     1621 cggccaggtc atcagcgagg acatcggccg caagctcgac agcaccaccg tcaaggacct
     1681 gggccgcgcc cgccgcgtcg tcgtgaccaa ggacgagacg acgatcatcg agggcaaggg
     1741 cgacgaggcc gcgatccgcg cccgcgtcga ccagatcaag accctgatcg agcagaccac
     1801 cagcgactat gatcgcgaga agctgcagga gcggctggcg aagctggccg gcggcgtggc
     1861 cgtcatcaag gtcggcgccg cgaccgagac cgagctgaag gaaaagaagc accgcgtcga
     1921 ggacgccctg agcgctaccc gcgccgcggt cgaagagggc atcgtgcccg gcggtggcgt
     1981 cgcgttgctg aacgcgctgc ccgccctgga ttcgatcaag gccgacggcg atatcgccac
     2041 cggcgtctcg atcctgcgcc gcgcgctcga agagccgatg cgccagatcg cgctcaacgc
     2101 gggccaggac ggcgcggtcg tggtcgagac ggtgcgccgc aaggcgaccg acgagaacaa
     2161 cgcctccgtc ggctacaacg tgctgagcgg ccagtatggc gacatggtgg cccagggcat
     2221 catcgacccg gccaaggtga cccgctcggc ggtggagaat gcggcttcga ttgccggcat
     2281 gatcctgacc accgaggccc tgatcaccga cattccggaa gagaagcccg cgatgccggc
     2341 cggcggcggc atgcccggcg gcggcatgga ttactaagtg ctgagtgctg agtgctgagt
     2401 gctgagtgtt taaagacgtt cgagtagtca gtggcttggc ttcgggctga gtaagttcgg
     2461 gaagtacgaa agagagtccc cgatccgttt gggtcgggga ctctttttgt gttttgcaac
     2521 gcgatgaata cagctaacat aaacaatctt cgtgtccttc gcgtcttcgc ggtgaacttc
     2581 cgtccggatg cgcgggctag ctgccgggct ggtcgcggat ctgcaccatg gcgtcgatga
     2641 cgacctggtt ctcctcgata gtgttgtaga aatagggcga gacgcggacc gcgccggggc
     2701 gcatgtctac gatggtgccc gcctgggcca gcgcggcgac caccgggcgc gggttgtcca
     2761 tgttgagcag cacgatgccc gaccgttgag ttgggtcctc ggccacgcgg ggcacgaacc
     2821 cggcggccag cgcgcgcgcg atcaggtctt cgctgaggtg ctgcgtgcgt tcgcgcagcg
     2881 cgggcggccc gatctcctgc acatacgaga ggccgccgag cagtgcgtag acggcggaga
     2941 tggacggcgt gccctgctcc agccgcccgg cgtcgggccg gtattcgagc gcgtggatgt
     3001 cgaagccgaa ctggttgttg acggcgaacc agccggggat ggtcggctcc agttgcggga
     3061 tcaactcgcg ccgggcgtag gtgaagacga tgcccgtgcc gcccagcagc cacttgagcc
     3121 cgcccgacag caggaaatcg acatcggcat ccttcacgtc ggtcgggatc tggccggtgg
     3181 cctggtaggc gtcgatcagc accagggcgc ccttgcggtg cgcgatctcg gcgatggcct
     3241 tgatgtcctg cacaaagccg ctggtgaaaa agacgtgtga cgtggcgacc agcgctgtgc
     3301 gctcatccac ggcccgctca aacgcctcca ggtccacgct cacgcggttg cgcgagggca
     3361 caaactccac ctcgacaccc tgcttgcgct tggcgagcca ctggtggccc agcgtgggga
     3421 agtcgaggtc ggccatgacc accttgttgc gcttgctata atccaggcag gacgcaatga
     3481 ccgacacggc gcccgagatt gacgggccga tagcgatctc gtccgccccg gcgttgatca
     3541 gcgcacccag gttggtccgc agcgcggcca gccggcccag ccagatctcg taccaggccg
     3601 acgcgcccat gctgccccac agcgtcaggt actcgttgac gccggccatg gcgcgcagcg
     3661 acagcgcgcc cagcgaacag gagttgagat agttcac
//
LOCUS       DASUBI010000028         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1116022, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000028 DASUBI010000000
VERSION     DASUBI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116022"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1295)
                     /gene="thrC"
                     /locus_tag="VFU72_00455"
     CDS             complement(45..1295)
                     /gene="thrC"
                     /locus_tag="VFU72_00455"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5421987.1"
                     /translation="MACGRVYAADQARARCDCGGLLDVELELGSIDPSVFDQRLLAMR
                     GVERSGVWRYRELLPPLPAHTIVSQPEGNTNLYERPELTRWAGVERLALKHEGENPTG
                     SFKDRGMTVAASHAKWTGARLVACASTGNTSASVAAYAALAGIPAIVFIPEGKISAGK
                     LGQTVAYGATIVQVRGDFDAAMALVQEASRAHGIYLLNSVNPFRIEGQKSIFFELLHQ
                     LDWELPDWIVLPGGNLGNSSALGRALADLHATGLIARQPRVAIVQAAGAAPFYHAWRG
                     GFRDYAPVVAETVASAIRIGDPVSVERARRTIEQTDGVVEIVSDEELLDAKALIDRAG
                     IGCEPASAASLAGTRKLASSGVIAPGAFVAGILTGNLMKDAETVMSYHLGPGSERRPL
                     ANRPVVIDPTLAALEGVLADALQR"
     gene            complement(1417..2373)
                     /locus_tag="VFU72_00460"
     CDS             complement(1417..2373)
                     /locus_tag="VFU72_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5421988.1"
                     /translation="MSETTEPRVQTPGRAQEVTALSQKAKKTRSLWSNALRQYRRHTL
                     AMAGTIVLLIIVLAVLFGPMLWPKDPFAFSFAESYKGVSLSHPMGTDDLGHDTFARVL
                     WGGRVSIAVGIVAMLVAIILGTLIGAVAGYFGGFIDSALMRLTDLFISLPELPLLLVV
                     IYLFQDTMKNRFGILIGTFVLIVGIIGALRWMTVARLVRAEFLSVKEKEYIEAAHSIG
                     VSTPSIIFKHILPNTLSPVIVAATLAVASAIITESTLSFLGLGFPPDIPTWGRLLNNA
                     NDFIQIAPHMVIFPGMAIFVTVLCINYVGDGLRDALDPRHTI"
     gene            complement(2408..>3139)
                     /locus_tag="VFU72_00465"
     CDS             complement(2408..>3139)
                     /locus_tag="VFU72_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5421989.1"
                     /translation="DFGYSFTSKSPVMGLIKQRLPTTLAVIGSAYLLSVLIAIPIGVL
                     SAIKQYSIMDNIATTIAFMGFSLPTFFTGLLAIMLFTIWLGWLPMIYSSTIKTPGLEG
                     LWLHIKQAIMPVLVLALFQTAALTRYVRASMLETIHQDYVRTARAKGLGEGVVINRHA
                     LRNALIPVVTIIALSIPGIFTGAVVTEQIFRVPGIGSLLISAINASDTPVIMAITFIF
                     AILVVIFNLVADVLYGVLDPRIKYS"
BASE COUNT          575 a          977 c         1086 g          501 t
ORIGIN      
        1 tcgtgtcgaa gccggagccg agattagcgc tggaggccgg cacgctaacg ctgaagcgca
       61 tcggccagca ctccctccaa cgccgccagc gtcggatcga tgaccaccgg ccggttggcc
      121 agcggccgcc gctcgctccc cggccccagg tgatagctca tcaccgtctc ggcgtccttc
      181 atcaagttgc cggtcagaat cccggcgacg aaggctccag gcgcgatcac gccggatgag
      241 gctagcttgc gcgtgccggc cagcgaggcg gccgaggccg gctcgcagcc gatgccggcg
      301 cggtcgatca gcgccttggc gtcgagcagt tcctcgtcgc tgacgatctc aaccactccg
      361 tcggtctgct cgatggtgcg gcgggcgcgc tcgacgctga ccggatcgcc gatgcggatg
      421 gcggaggcga ccgtttcagc cacaaccggg gcgtagtcgc ggaagccgcc acgccaggcg
      481 tggtagaagg gcgcggctcc ggctgcctgc acgatggcga cgcgcggctg gcgcgcgata
      541 agtccggtcg catgcagatc ggccagggcg cggcccaggg cgctggagtt gcccaggttg
      601 ccgccgggga gcacgatcca gtccggcagc tcccagtcga gctggtgcag cagctcgaag
      661 aagatcgact tttggccctc gatgcgaaac ggattgaccg aattcagcag atagatgccg
      721 tgggcccggc tggcctcttg caccagcgcc atagccgcgt cgaagtcacc gcgcacctgg
      781 acgatggtgg cgccgtaggc gaccgtctgc cccagcttgc cggccgagat tttgccctcg
      841 gggatgaaga cgatcgccgg gatgccggcc aatgcggcgt aggcggcgac ggaggctgag
      901 gtgttgccgg tcgaggcgca ggcgaccagg cgggcgccgg tccacttggc gtgactggcg
      961 gccacggtca tgccccggtc cttgaaggag ccggtggggt tctcgccttc gtgcttcagc
     1021 gccagccgct cgacgccggc ccagcgcgtc agctccggcc gctcgtagag gttggtgttg
     1081 ccctctggct gactgacgat cgtatgcgct gggagtggcg gcagcagctc gcggtagcgc
     1141 cagacgccgc tgcgctcgac accgcgcatt gccagcagcc gctggtcgaa gacgctcggg
     1201 tcgatactgc cgagctccag ttcgacatcg agcaggccac cacagtcgca gcgcgcccgc
     1261 gcctgatcgg cggcgtagac gcggccacat gccatacacc gaagctgcac ggagttcccc
     1321 tcttcgcctt caccacaccc acacaatgat ggcacgggcg cgtgggcccg tgccatcaga
     1381 ttgaaccaga ttccgccagc cgcgcggcgc cgcgctctag atcgtatggc gcgggtcgag
     1441 cgcgtcgcgc agaccgtcgc cgacgtagtt gatacagagc acggtgacga agatcgccat
     1501 gccggggaag atcaccatgt gcggcgcgat ctggatgaag tcgttggcat tgttcagtag
     1561 ccggccccag gtcgggatat ccggcgggaa accgagtccg aggaacgaga gggtcgactc
     1621 ggtgatgatg gccgaggcga ccgccagcgt cgcggccacg atgaccgggc tgagcgtgtt
     1681 gggcaggatg tgcttgaaga tgatgctggg ggtgctgacg ccgatgctgt gggccgcctc
     1741 gatatactcc ttctccttga cggagaggaa ctcggcacgc actagacgcg cgacggtcat
     1801 ccagcgcagc gcgccgatga tgccgacgat cagcacgaag gtgccgatca ggatgccgaa
     1861 gcgattcttc atcgtatcct ggaagaggta gatgaccact agcagcagcg gtagctccgg
     1921 cagcgagatg aagaggtcgg ttagccgcat cagcgcggaa tctatgaagc cgccaaaata
     1981 gccggcgacc gcgccgatca gcgtgccgag gatgatcgcc accagcatgg cgacgatgcc
     2041 gaccgcgatc gagacgcggc cgccccagag gacgcgcgcg aaggtgtcgt ggccgaggtc
     2101 atcggtcccc atcgggtggg acagcgagac gcctttgtag gattcggcga agctgaaggc
     2161 gaaggggtcc ttgggccaga gcatcgggcc gaagaggacg gcgagcacga taatcaggag
     2221 cacgatggtg ccggccatgg ccagcgtatg tcgccggtat tggcgcagcg cgttcgacca
     2281 caacgagcgc gtcttcttgg ccttctggct cagggcggtg acctcctgag cgcggccggg
     2341 cgtttgcacc ctgggttcgg tcgtctcaga catctatggc gcctccctgc tctagcgtgg
     2401 cgatcgctca ggagtacttg atacgaggat caagcacgcc gtacagcacg tcggccacca
     2461 aattgaaaat caccaccagg atcgcgaaga tgaacgtgat cgccatgatc accggcgtat
     2521 cgctggcgtt gatggcggag atcaagagcg agccgatccc cggcacgcgg aagatctgct
     2581 cggtcaccac cgcaccggtg aagatgccgg ggatgctcag ggcgatgatc gtgaccaccg
     2641 ggatcagcgc gttgcgcagc gcgtgccggt tgatgaccac gccctcgccc aggcccttcg
     2701 cccgcgcggt gcgcacgtag tcctgatgga tcgtctctag catcgaagcg cgcacgtagc
     2761 gcgtcagcgc ggcggtctgg aagagcgcca gcaccaggac cggcatgatc gcctgtttga
     2821 tgtggagcca gagcccctct aagccgggcg tcttgatcgt cgagctgtag atcatcggca
     2881 gccagccgag ccagatggtg aaaagcatga tggccagcag gccggtgaag aaggtgggca
     2941 acgagaagcc catgaaggcg atcgtggtgg cgatgttatc catgatcgag tactgcttga
     3001 tcgccgagag cacgccgatc gggatggcga tcaaaaccga gaggagatag gcgctgccga
     3061 tcacggccag ggtcgttggc agccgctgct tgatcagccc catcaccggc gacttgctgg
     3121 tgaaggagta gccgaagtc
//
LOCUS       DASUBI010000029         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1266773, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000029 DASUBI010000000
VERSION     DASUBI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266773"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1128)
                     /locus_tag="VFU72_00470"
     CDS             complement(148..1128)
                     /locus_tag="VFU72_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5421990.1"
                     /translation="MALIEVEQLQKRFRVARRKRGPLGGVRSLLAREVHEIVAVDDIS
                     FAVERGEMLGYIGPNGAGKSTTIKMLTGILVPSGGRVVVDGLVPWRQRRQLAGRIGVV
                     FGQRTQLWWDLPLIESFRLLRHVYRVPEERYRRNLCRLSELLDLTPFLEQPVRQLSLG
                     QRMRGDLAAAMLHEPLILYLDEPTIGLDVVAKERVRQFLRELNRETGVTVLLTTHDMG
                     DIARLCERVLIIDHGRLLYDGGVAQITERYGGERRLVVDLDEEVPLPEVPGARLERRE
                     GNRLWLSFPRELRAPELIACLTARYRIRDLTLEEPDIEQVVRRIYQEGIV"
     gene            complement(1130..1930)
                     /locus_tag="VFU72_00475"
     CDS             complement(1130..1930)
                     /locus_tag="VFU72_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5421991.1"
                     /translation="MASASIGLRLIGGQIRGQVQYRASFLLATTGSFLSTFVEVLAIL
                     ILFSQFHTLAGWTVAQVALLYGLVAVAWGVVDMIGGGFENLSTLIRRGEFDRFLLRPV
                     PAPLQVVAFDFQLRRIGRVSDGLLALGLAWWWGALDWSLPRLLVLLAAIVSTAVVFFT
                     VLVIAAAICFWTIESSEAQNIFTYGGTELASYPMSIYHPLLRGLFLYLVPLALTSYEP
                     ALYILGKPDALGLPEVVRFLAPLVAGAFFALGYGLWQLGLRHYQSTGS"
     gene            complement(1923..2714)
                     /locus_tag="VFU72_00480"
     CDS             complement(1923..2714)
                     /locus_tag="VFU72_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5421992.1"
                     /translation="MRVYLEAMRLAYRRYRTYGAATLAGLATNAFFGVLRSYVFIALY
                     HNRGVTQGFSLHDAVSYVWLSQAMIMPLYLWSWFEIADTIRSGDVITDLSKPFDYFVY
                     WLSRDLGRAAYHLLYRWLPTVLMGAILFGLRVPHNPLTVLWFALSLLMAVVLSFCVRF
                     AINISGFWSTDVRGLYGICFLFVNFFSGFLVPLAFFPPTLRALSQSLPFAGMISIPLT
                     IFLEQAHGVELAWLLLEQALWLAAFVVGARLLLRAAVRKLVVQGG"
     gene            2933..3466
                     /gene="cpt"
                     /locus_tag="VFU72_00485"
     CDS             2933..3466
                     /gene="cpt"
                     /locus_tag="VFU72_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT"
                     /protein_id="HEU5421993.1"
                     /translation="MTTQIIVLNGTSSSGKTGIARCLQAILPQPWLRVGIDDLLDALP
                     TSLMGSASGFVVGPQGEVSLGKRFLEIETAWWTGVAGMAQAGARIIIDDVFLGGAASQ
                     VRLRAHLQGLTVLWVGVRCDPKIAAGREIARGDRMIGMAAAQAKLVHDGVVYDIEVDT
                     SHAESLHCARAIAEQVA"
     gene            complement(3507..>3845)
                     /locus_tag="VFU72_00490"
     CDS             complement(3507..>3845)
                     /locus_tag="VFU72_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5421994.1"
                     /translation="ADVLVIKPMLAGGLRRGRAIVELAHAAGLRAIVTTTIDSGVGIA
                     AALHLAATLPAPPLACGLATRSLLGADLVSGLPPVVAGSLHLPDGPGLGVMLSEAGCG
                     GAAASVRFGR"
BASE COUNT          692 a         1313 c         1326 g          514 t
ORIGIN      
        1 ccagttgccg cgctgcccgc tcagccgccc gatccagttc ggcgaagctc cagcgcacgc
       61 cgggcgccag cagtgcggtc cgccccggtg tgacagcggc gcggtaggcg agccagtcgg
      121 gcacgaggcc tggcacaagc tccatggtca gacgatcccc tcctgataga tgcgacgcac
      181 cacctgctcg atatccggct cctccagcgt caggtcgcgg atacggtagc gcgcggtgag
      241 gcaggcgatc agctccgggg cgcgcagctc gcgcgggaag gagagccaga gacggttccc
      301 ctcgcgccgc tccagccgcg cgccgggtac ctctggcaga ggcacctcct cgtccagatc
      361 gaccaccagc cgccgctcgc cgccgtagcg ctcggtgatc tgcgccaccc cgccatcata
      421 gagcagtcgc ccgtggtcga tgatcagtac gcgctcgcaa aggcgcgcga tgtcgcccat
      481 gtcgtgcgtc gtcaagagca ccgtgacccc ggtctcgcgg ttcagctcgc gcagaaactg
      541 gcgcacgcgc tccttggcca ccacgtccag cccgatcgtc ggctcgtcga gatagaggat
      601 cagcggctcg tgcagcatgg cggcggccag atcgccgcgc atgcgctggc ccagcgagag
      661 ctggcgcacc ggctgctcga ggaacggtgt caggtcgaga agctcgctca ggcggcagag
      721 gttgcgccgg tagcgctcct ccggcacgcg gtagacgtgg cgcaggaggc ggaagctctc
      781 gatcaagggc agatcccacc agagctgggt gcgctggccg aagacgacgc cgatgcgccc
      841 ggccaactgc cgccgctgcc gccaggggac gagcccatcg accaccacgc gcccgccgct
      901 cggcaccagg atgccggtca gcatcttgat ggtagtgctc ttgccggcgc cgttcggccc
      961 gatgtagccg agcatctcgc cgcgctcaac cgcgaagctg atgtcatcca ccgccacgat
     1021 ctcgtgcacc tcgcgcgcca gcagcgagcg caccccgccc agcggccccc gcttgcggcg
     1081 agccacgcgg aagcgcttct gcagctgctc gacctcgatc agcgccatct cagctccccg
     1141 tgctctggta gtggcgcagg ccgagctgcc agaggccata gccgagcgcg aagaaggcgc
     1201 ccgccaccag cggcgccagg aagcgcacca cctccggcag ccccagcgcg tcgggcttgc
     1261 ccaggatgta gagcgccggc tcatagctgg ttagcgccag tggcaccaga tagaggaaga
     1321 ggccgcgcag cagtgggtga tagatgctca tcgggtagct ggccagctcg gtgcccccgt
     1381 aagtgaagat gttctgcgcc tcgctgctct cgatggtcca gaagcagatg gcggccgcga
     1441 tcacgagcac ggtgaagaag acgaccgccg tgctgacgat cgccgccagc agcaccaaca
     1501 gtctgggcag cgaccagtcg agcgcgcccc accaccaggc tagcccgagt gccagcaggc
     1561 cgtccgagac gcgcccgatc cggcgcagct gaaagtcgaa tgccaccacc tgcagcggcg
     1621 ccggcaccgg ccgcagcagg aagcggtcga actcgccgcg gcgaatcagc gtggagaggt
     1681 tctcgaagcc gccgccgatc atatcgacca cgccccaggc gactgccacc aggccataga
     1741 gcagcgctac ctgcgccacc gtccagccgg ccagcgtgtg gaactggctg aagaggatca
     1801 ggatcgccag cacctcaacg aacgtgctga ggaagctgcc ggtggtcgcc agcaggaagg
     1861 aggcgcggta ttggacctgg ccgcggatct ggccgccaat cagccgcagg ccgatgctcg
     1921 cgctagccac cctgcaccac cagcttgcgt accgccgcgc gcagcagcag ccgcgcgcct
     1981 actacgaagg ccgccagcca gagcgcctgc tccagtagca gccacgccag ctcgacgccg
     2041 tgcgcctgct ccaggaagat cgtcagcggg atcgagatca tgccggcgaa ggggagcgac
     2101 tgggagagcg cccgcaaagt cggcgggaag aaagctaagg gaaccaagaa gccactgaag
     2161 aagttcacga agaggaagca gatgccatag agaccgcgca cgtcggtgct ccagaagccg
     2221 ctgatattga tcgcgaagcg cacgcagaag ctgagtacga ccgccatcag caggctaagc
     2281 gcgaaccaga gtacggtcag cgggttgtgc ggcacgcgca ggccgaagag gatcgcgccc
     2341 atgagcacgg tcggcaacca gcggtagagg aggtggtagg cggcgcggcc gaggtcgcgg
     2401 ctaagccagt agacgaagta gtcgaaaggc ttggagaggt cggtgatgac gtcgccggag
     2461 cggatcgtat cggcgatctc gaaccagctc cagaggtaga gcggcatgat catcgcctgg
     2521 ctgagccaga cgtagctgac cgcgtcgtgc agcgagaagc cctgcgtaac gccgcgattg
     2581 tggtagaggg cgatgaagac gtaggagcgc agcacgccga agaaggcgtt ggtggccagg
     2641 ccggcgagcg tcgccgcgcc ataggtgcgg tagcggcgat acgccagccg catcgcctcc
     2701 aggtacacgc gcaccgcacg ctcctcgact ctaccgggat cgccccgcac tgagacagga
     2761 ccgctgatga tgccacatcg gcggtcgagc gtgcatccgc cgcgtggtcc ccctcccccg
     2821 aggctgtgat acgatcgcga gctcgtggcg aggtggtcgc ggctgctccc gcggtctgcg
     2881 acgagcaccg cgccaaacgg agcaccgtga gcggggaagg cgtggcgatc cggtgacgac
     2941 gcagatcatc gtgctgaatg gcacgtccag ctccggcaag acgggcatcg cccgctgcct
     3001 gcaggcgatc ctgcctcagc cgtggctacg cgtcggcatc gacgatctcc tcgacgcgct
     3061 gccgacgtcg ctcatgggct cggcctcggg ctttgtggtc gggccacagg gcgaggtctc
     3121 gctcggcaaa cgctttcttg aaatcgagac cgcctggtgg accggggtgg ccgggatggc
     3181 ccaagccggt gcgcggatca tcatcgacga cgtcttcctc ggcggcgccg cctcccaggt
     3241 gcgcctgcgc gcgcatctgc aggggctcac ggtgctctgg gtcggggtac gctgtgatcc
     3301 caagatcgcc gctggccgtg agattgcccg gggcgaccgg atgatcggga tggccgccgc
     3361 gcaagcaaag ctggtacacg acggggtcgt ctacgatatc gaggtcgaca cgagccacgc
     3421 cgagtccttg cactgcgccc gcgccatcgc ggagcaggtg gcctaagcgt cccagcgtcg
     3481 attctccccg gttcgggccg gggaggttat ctgccgaatc gtaccgatgc cgccgcgcca
     3541 ccgcacccgg cctcgcttag catcacgccg aggccgggac cgtccggcag atgcagcgag
     3601 ccggcgacga ccggcggcag gccggagacc agatccgcgc ccagcaagga gcgtgtggcc
     3661 aggccgcagg cgagcggcgg agcgggcagc gtcgcggcga ggtgaagggc ggcggcgatg
     3721 ccgacgccgc tgtcgatggt ggtggttacg atggcgcgca gaccggcggc gtgcgccagc
     3781 tcgacgatcg cgcgcccacg ccgcagccct cccgccagca ttggcttgat gaccagcacg
     3841 tcggc
//
LOCUS       DASUBI010000030         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_67136, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000030 DASUBI010000000
VERSION     DASUBI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67136"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFU72_00495"
     CDS             <1..1149
                     /locus_tag="VFU72_00495"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5421995.1"
                     /translation="PLSQAALQRIIDDSMLPKLKQDDIAGGLAAGLDATASQITNPVP
                     VAARSPGMETLARWVRLPLNLLALAGALAVGWLISRLRRARPRSSQPIVATANPPSDT
                     PPATVGALVQGRITDSELAGAILELGRRGPLSVEPESKSKIRIQLGKRAAARTEVEAQ
                     LWDALARLAGPDGVVDSGELARLPHEWRGIKETLREQLIAAGLYNARAADLRRPGYLV
                     GAAALIGATVLGILAIASREPWGAAGAGALAIAGIAVIIYAYDMPGTTEAGETEAAHW
                     RGYLAGLKDLGRRSPASLQEDVDLDVALPYLLAMGKANLLNKRLKEASEAGFNPAWLS
                     RSLQPDQNRAGFYPVWVAYMGSLAPTSSGAGSMAGGAAAGGGGAGGSF"
     gene            complement(1251..1514)
                     /gene="ffs"
                     /locus_tag="VFU72_00500"
     ncRNA           complement(1251..1514)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFU72_00500"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1580..3457)
                     /locus_tag="VFU72_00505"
     CDS             complement(1580..3457)
                     /locus_tag="VFU72_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5421996.1"
                     /translation="MSDEVRTPEWLLAARQTTGDSGAREHLGQLATLHGVQAAARGVA
                     LREVYAALLASWAAAGEPADAEVLAAVSQGYAEARDLGASGGHPLSGALSRLTALHRI
                     SRAATASLDLDGMLRTVVHVVRETMAADSGSIFLYDAGSNTLVLRASVGLNPEATGMV
                     TLPLGAGITGIAAVKREIVHAPDAARHPAYLSYPLIGDTVYTSHVSVPLTLRSPSRLV
                     GVLNILSRQRRVFDRDELAFLQTAADEIAIAIENAQLYNQTDAELRQRISQLALLQRL
                     TKTLAATLDLPELLRLVGEQAVALTLAAAVEIYRVPRDGAAGLDLLSQFPEERARELE
                     AANEAVRLLVDDAMESAAPRRRAGRGDELDVEVLPLLTGRRPVGAICLFFAAETPAAP
                     ESLLLAFCDVVAMALENAELYQEARRGYTRASVLLQEMHHRVRNNLQTVAALLSMQAR
                     HADDAGWSEPLREAVSRIQSIAAVHDVLTGENLRETTLETLARHVAKEASATLIPTGS
                     RIELVVKSSPVRVGSREATVLALLINEFVANAVLHGLAGRERGRVTIGARARAGQVEL
                     TVADDGRGLPAEFALDRAGLGLQIARGLVHDDLGGELELSGNPEGGATARVTFRSSLT
                     G"
     gene            3506..5113
                     /locus_tag="VFU72_00510"
     CDS             3506..5113
                     /locus_tag="VFU72_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5421997.1"
                     /translation="MDERARSLGVVTEGSFSAGLTVRLAPDSPTESLRVGSFVVLEGE
                     NNRYFSLITDLRLRMTDAGLAASPPERRSAFLRRVLSGSHTYATAEVRPDLMLENVHD
                     LTDTARPGPVRNIPMHFAEMRLARPEDFDLVFGAESATRFALGSPPAMDIRVPIDLEE
                     FVTRSNGIFGQSGTGKSVLTRLLLFGIMKSRTASVLLFDMHGEYARRPAGAPELAGLV
                     DLLGSSRIREYSLDPAMTSGRDLRIGLNQIEPEDIELLAGELNLTGTFGATTHQLWSR
                     FHQKWLATLLEMSAEEIKEFCAETLTHEGALSALKQKLAVLERKEYLLRNAETRTIDD
                     ILIHLQKGEHVILTFGRYQSLIDYMLVANIVTRRIHQRYTDQVLRYEGSGGRDEEPRR
                     LLVVLEEAHKFLAPEAARQSIFGTIAREMRKYAVTLLVVDQRPSGIDPEVLSQLGTRV
                     TGLLTDSQDIDAVLTGAADRSRMRGLLASLRPRQECLILGHAIPMPIVLRTRDYNYDE
                     LMAEARDAGVGSVTKDDLAFLYGETDE"
     gene            5120..>5552
                     /gene="sbcD"
                     /locus_tag="VFU72_00515"
     CDS             5120..>5552
                     /gene="sbcD"
                     /locus_tag="VFU72_00515"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEU5421998.1"
                     /translation="MKLLHFADLHLGIENYGALNPRTGLSTRVDDFLAALERIIDAAL
                     AERVDAVLFAGDAFKNRDPNPTLQRAFAERIRRLAAAEIPTVLLIGNHDLPNITARAT
                     AIDIYEALAIPNIFVARNLGRLDIPTRSGPLQVVALPWVARN"
BASE COUNT          887 a         2017 c         1794 g          854 t
ORIGIN      
        1 ccgctctcgc aggctgcgct gcagcggatc atcgacgact ccatgctgcc gaaactgaag
       61 caggacgata tcgcgggcgg gctcgccgcc gggctcgacg cgacggcgag ccagatcacc
      121 aatcccgttc ctgtggccgc ccgcagcccc ggcatggaga cgctcgcccg ctgggtgcgg
      181 ctgccgctga acctgctggc gcttgccgga gcactcgccg tcggctggct catctcccgc
      241 ctgcgtcgcg cacggccccg ctcgtcccag ccgatcgtcg cgacggcgaa cccgccgtcc
      301 gatacgccgc ccgcgaccgt gggcgcactg gtgcaaggtc gcatcaccga ctcggagctg
      361 gccggggcca tcctcgaact gggccggcgc ggtcctctga gtgttgagcc ggagagcaag
      421 agcaagatcc ggatccagct cggcaagcgc gcggccgctc gcaccgaggt cgaggcgcaa
      481 ctctgggacg cactggcgcg gctggccgga ccggacgggg tcgtcgattc cggcgagctc
      541 gcgcggctcc ctcacgagtg gcgcggcatc aaagagaccc ttcgtgagca gctcatcgca
      601 gctggcctct acaacgcacg cgccgccgac ctgcgccggc cgggctatct cgtcggggcc
      661 gcagcgctca tcggcgcgac ggtgctcggg attctggcga tcgcctcgcg cgagccctgg
      721 ggcgcggccg gcgccggtgc gctggccatc gccgggatcg cggtgatcat ctacgcctac
      781 gacatgccgg ggacgaccga agccggcgag accgaggccg cgcactggcg cggctacctg
      841 gcgggcctga aggatctcgg caggcgttcg ccggccagcc tgcaggaaga tgtcgatctc
      901 gacgtcgcgc tgccctacct gctggcgatg ggcaaagcca atctcctgaa caagcggttg
      961 aaggaggcga gcgaggccgg cttcaatccc gcctggctct cgcggtcgct ccagccggat
     1021 cagaaccgcg ccggcttcta tccggtttgg gttgcctata tgggctcgct cgcgccgacc
     1081 agctcaggcg ccggtagcat ggcgggcggc gcggcggccg gcggcggggg agccggtggc
     1141 agcttctgag cgctcaccca agcggcagca aacggccgcg ccgccgtgtc tatgcaagga
     1201 cacaaaagcg ggcggtcaga ctgaccgccc gctctcgctg tcatgaaagg tcgtgcaccc
     1261 ttcgtcgatg ccgatcctcc ggcttgcctc agactagtta gctccggcca ggcgacccca
     1321 cggcacccag cggtgaccac ttacggctgc ttccttccgg acctgaccgg gttcgcgggc
     1381 cgctgccgcg tgggacccaa ccatcctcgc cacttggccc gaacagtccc acagaatcgg
     1441 acctctaaag ggaattcagc cccgctacag cggattgcgg gtatagggca ccgctagttc
     1501 cccgcttagc acgaccgaac taatcatacc cagctggtac ccttgattca agtgtacgga
     1561 cgagcctgat gtcccggtat cagccggtca acgaagagcg gaaagtcact cgtgccgtcg
     1621 cacctccctc ggggttgccg ctcagctcca gctccccgcc gaggtcgtcg tgcaccagac
     1681 cgcgcgcgat ctgcaggccg agcccggcgc ggtcgagcgc gaactctgcc ggtagcccgc
     1741 ggccgtcgtc ggccacggtc agctccacct gcccagcgcg agcgcgtgct ccaatcgtca
     1801 ctcgcccgcg ctcccgcccg gccaggccgt gcaggaccgc gttcgccacg aactcgttga
     1861 tcaggagcgc cagcactgtc gcctcgcgcg agccgacgcg gaccggggat gacttgacca
     1921 ccagctcgat ccggctgcca gtcgggatga gcgtcgcgct cgcctccttc gcgacgtgtc
     1981 gcgccagtgt ctccagcgtc gtctcacgca gattctcacc ggtcaggaca tcgtgcaccg
     2041 cggcgatgct ctggatgcgg ctgacggcct cgcgcagcgg ctcggaccag cccgcgtcgt
     2101 cggcgtgccg cgcctgcatc gagagcagcg cggccaccgt ctgcaggttg ttgcgcacgc
     2161 ggtgatgcat ctcctgcaag agcaccgagg cgcgcgtgta gccgcggcgc gcctcctggt
     2221 agagctcggc gttctccagc gccatcgcta ccacgtcgca gaatgcgagg agcagcgact
     2281 ccggtgcggc gggcgtctcg gcggcgaaga agaggcagat cgcacccacc gggcgtcgcc
     2341 cggtcaggag aggcagcacc tcgacgtcca gctcgtcgcc gcgaccggcg cgacggcgcg
     2401 gcgcggccga ttccatcgcg tcgtcgacca gcagccgcac cgcttcgttg gccgcttcca
     2461 gctcgcgcgc ccgttcctcc gggaactggc tcaagaggtc gagaccggcg gcaccatcgc
     2521 gcggcacgcg gtagatctcg acggccgccg ccagcgtcag cgcgaccgcc tgctcgccga
     2581 ccaggcgcag cagctccggc agatcgagcg tggccgccaa cgtcttggtc agccgctgaa
     2641 gcagcgccag ctgcgagatg cgttgccgca gctcggcgtc cgtctgattg tagagttgcg
     2701 cgttctcgat ggcgatcgcg atctcgtcgg ccgcggtctg aaggaaggcc agctcgtcgc
     2761 ggtcgaagac gcgccgctgc cggctcagga tgttgagcac gccgaccagc cggctcggcg
     2821 agcgcagcgt cagcggcacc gagacatggg aggtgtagac ggtgtcgccg atcagtgggt
     2881 agctcaggta ggccgggtga cgcgcggcgt ccggcgcatg cacgatctcg cgtttgaccg
     2941 ccgcgattcc ggtgatcccg gcccccaatg gcagcgtcac catcccggtc gcctcgggat
     3001 tcaaccccac cgaggcacgc agcacgagcg tgttcgagcc ggcgtcgtag aggaagatcg
     3061 agccggaatc ggccgccatc gtctcgcgca cgacgtggac gaccgtgcgc agcatgccgt
     3121 caaggtcgag actggcggtg gccgcgcggc tgatgcggtg cagcgcggtc aggcgcgaga
     3181 gggcgccgga gagcgggtgc ccgccgctgg cgcccaggtc gcgcgcctcg gcatagccct
     3241 gcgataccgc cgccagcacc tccgcgtccg ccggctcgcc ggccgccgcc caggatgcaa
     3301 ggagcgcggc atacacctcg cgcagcgcga caccccgcgc cgcggcctgc acgccatgca
     3361 gcgtcgccaa ctgacccagg tgttcccgcg ctccgctatc tcccgtcgtc tggcgtgccg
     3421 ccagcagcca ttccggcgtc cgaacctcat cactcatggt aagattaccc tgtccgtact
     3481 ggcgcgaacg gccggaaggg gtcgcatgga cgagcgtgcg cgatcgctcg gagtcgtcac
     3541 cgagggcagc tttagcgctg gcctcaccgt tcgtctcgct cctgactctc ccaccgagtc
     3601 gctgcgcgtg ggcagcttcg tcgtgctcga gggcgagaac aaccgctatt ttagcctgat
     3661 caccgatctg cggctgcgca tgaccgacgc cgggctggcc gccagcccgc ccgaacgccg
     3721 ctccgctttc ctgcggcggg tcctgtcggg cagtcacacc tacgccaccg ccgaggtgcg
     3781 ccccgatctg atgctcgaaa acgtccacga cctgaccgat acggcccggc cggggccggt
     3841 gcgcaacatc ccgatgcatt tcgccgagat gcgcctggca cgtccggagg acttcgacct
     3901 cgtcttcggc gccgagagcg ccacgcgctt cgccctcggc tcaccaccgg ccatggatat
     3961 ccgcgtcccg atcgatctcg aagaattcgt gacgcggagc aacggcatct tcggacagag
     4021 cggcacgggc aagagcgtgc tcactcgcct cctcctcttc ggcatcatga agagccgcac
     4081 cgcttccgtc ctcctcttcg acatgcacgg cgagtacgcc cgccgcccgg ccggcgcgcc
     4141 ggagctagcc gggttagtcg atctcctcgg cagcagtcgc atccgcgagt actccctcga
     4201 tcccgccatg acctcgggcc gcgatctgcg catcggcctc aaccagatcg aacccgagga
     4261 tatcgagctg ctggccggcg agctgaatct gaccggcacc ttcggcgcca ccacccacca
     4321 gctctggtcg cgattccatc agaagtggct ggctacgctc ttggagatga gcgcggaaga
     4381 gatcaaggag ttctgcgctg aaaccctcac acacgagggg gcgctatcgg cgctcaaaca
     4441 gaagctggcg gtgctggagc gcaaagagta cctgctgcgc aacgccgaga cccgcaccat
     4501 cgacgacatc ttgatccact tgcagaaggg cgagcacgtc atcctcacct tcggccgcta
     4561 ccagtcgctc atcgactaca tgctggtggc caacatcgtc acccggcgca tccaccagcg
     4621 ctacaccgat caagtgctgc gctacgaagg gtccggcggc cgggatgagg agccgcgccg
     4681 cctgttggtg gtgctggagg aggcgcacaa gttcctggcg cccgaggcgg ctcgccagtc
     4741 gatcttcggc accatcgcac gcgagatgcg caagtacgcc gtgacgctgc tggtcgtcga
     4801 ccagcgccca tccggcatcg accccgaggt gctctcgcag ctcggtacgc gcgtcaccgg
     4861 gctattgacc gacagccagg acatcgatgc cgtgttgacc ggtgcggccg accgtagccg
     4921 catgcgcggc ctgctggcca gcctgcgccc gcgccaggaa tgcctgatcc tgggccatgc
     4981 catccccatg ccgatcgtgt tgcgcacgcg cgactacaat tatgacgagc tgatggccga
     5041 ggcgcgcgac gccggcgtcg gcagcgtgac gaaagacgac ctggccttcc tatatggcga
     5101 gaccgacgaa taggacgtga tgaagctcct gcacttcgcc gatctgcacc tcggaatcga
     5161 gaactacggc gcgctcaatc ccagaaccgg actctcgacg cgtgtcgacg acttcctggc
     5221 ggccctggaa cggatcatcg atgccgccct tgccgagcgg gtcgacgccg tcctcttcgc
     5281 cggcgacgcc ttcaagaacc gcgatccgaa ccccaccctg cagcgcgcct tcgccgagcg
     5341 catccgtcgc ctggccgcgg ccgagatccc aaccgtgctc ctgatcggca accacgacct
     5401 gccaaacatc accgcgcgcg ccaccgccat cgatatctac gaggcgctgg cgatcccgaa
     5461 catcttcgtg gcccgcaatc tcggccgtct tgacattccg actcgcagcg gtccgcttca
     5521 ggtggtcgcg ctcccctggg tcgcccgcaa cc
//
LOCUS       DASUBI010000031         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1075866, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000031 DASUBI010000000
VERSION     DASUBI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075866"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFU72_00520"
     CDS             complement(<1..659)
                     /locus_tag="VFU72_00520"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5421999.1"
                     /translation="MTDNQTRATRSHWTHEKTEAVAREGMVAAMQPLAAEAGAEMLRR
                     GGNAVDAAVATAFAVGVVEPFMSGVGGIAFLTYRDATSGRTSCLDGSTVLPAAIRPEM
                     FELLPGGQRAGMYGWRATKDDANNTGWRSPGVPGTPALLGEAHRRYGRLPWSDVLRPA
                     IQLAEEGFPVNHYVAMMIAANYDLLSRFPESRRTFIKPSGAPLSAVRGDRLRQPDLAR
                     TL"
     gene            1090..2214
                     /locus_tag="VFU72_00525"
     CDS             1090..2214
                     /locus_tag="VFU72_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5422000.1"
                     /translation="MLGGFLPHHFFSFSGSARGGLATPVTSTPAANLAPDGASLAPGP
                     PASSTPAPSVGPTPAPVAAATPTPVRTPQPASPVASPSAGATPTPAPLPLSALPPALR
                     GLFVAAPVSGLSCQPPPPDDYASCRFTESSGLSMTFYLFLPQSFDPARRYPLVLLLEG
                     GGERADATNTPEQNREKLLSDPYAAVWGPGVTIPDAPQVQRGHPSFVVIPQVPAPQFY
                     MSVPANTGSYTLAPLPTDALRTAKEIVDALQQAVAQVDPDRLYVTGLSMGGYGAWEMI
                     ERYPGYFAAAAPICGGGDPSLAVRLVDLPIWAFHSADDPIVPVQATRDMIAAIEAAGG
                     QPRYTEFTDAGHGAWVPAYGIGGLPSPVADFYDWLFAQHK"
BASE COUNT          369 a          938 c          860 g          440 t
ORIGIN      
        1 agggtgcggg ccaggtcggg ctggcgcagg cggtcgccgc gcaccgccga gaggggcgcg
       61 ccgctcggct tgatgaaggt gcgccggctc tcggggaagc ggctgagcag gtcataattc
      121 gccgcgatca tcatggcgac gtagtggttg accggaaaac cctcctccgc cagttggatc
      181 gccggccgga ggacatcgct ccaggggagc cggccgtagc gccggtgcgc ctcccccagc
      241 agcgccgggg tgccgggcac gccgggcgag cgccagccgg tgttgttggc gtcgtctttg
      301 gtggcgcgcc agccgtacat cccagcgcgc tggccaccgg gcaacagctc gaacatctcc
      361 ggccggatcg ccgcaggcag cacggtcgag ccgtcgaggc agctcgtccg gccgctggtg
      421 gcgtcgcggt aggtgagaaa cgcaatgccg ccgacgccgc tcatgaaggg ctcgaccacc
      481 cccaccgcga aggcggtggc gaccgcggcg tcgacggcgt tcccgccgcg ccgcagcatc
      541 tcggcgccgg cctcggcggc cagcggctgc atcgcagcta ccatcccctc gcgcgctacc
      601 gcctcggtct tctcgtgggt ccagtggctg cgcgtcgctc gcgtctggtt gtcggtcatg
      661 gttgctgctc actctcatgg aatacgtcga gggtcacttg gcgcacggat ccctccacct
      721 cggtcgggat gacaggggga gtaaagagct gacaaattgc gattatcatc gtcgtccgcc
      781 cccatccccc gcacatgtca tcctgagctt gtcgaaggat ccgtgcctca ccggttcccc
      841 atgctcttcc ttcgccgccg tcgctctcac gcgaccttgt atgccagggg caatatctcg
      901 tcaagcgcgg ccgggggcat gcccgcccag cttgcggcgc cggttacaat cgaaggcggc
      961 tcgcgtctgt ctcgctggat tgtcgccggc ttggtgcccg ggtttcacct gtcgcgccgt
     1021 taggggagaa caacgcgccg gcagagtcgc tcatcgatcg ccgtcgtcct gctggtcctg
     1081 ctggtagccg tgctcggcgg cttcctgccg caccactttt tcagcttcag cggcagcgcc
     1141 cgcggcggcc tcgcgacgcc ggtcacctcc acgccagctg ctaacctcgc gcccgatggg
     1201 gcgtcgctcg cgccgggacc gccggcctcg tcgacgccgg ctccgagcgt gggtccgact
     1261 cccgcgcccg tcgcggccgc gactccgact cccgtgcgca ccccgcagcc agcctcaccc
     1321 gttgcctcac cgtccgccgg cgccaccccc accccggcgc cgctgccgct gtcggcgctg
     1381 ccgcccgcgc tgcgcgggct gttcgtggcc gcgccggtga gcgggctcag ttgtcagccc
     1441 ccgccgcccg acgactacgc cagttgtcgc ttcaccgaat cgtccgggct gtcgatgacc
     1501 ttctacctgt tcctgccgca gagcttcgat ccggcgcggc gctatccgct ggtgctcctg
     1561 ctggagggcg gcggagagcg tgccgatgcg accaacaccc ccgagcagaa tcgcgagaag
     1621 ctgttgtccg acccctacgc ggcggtctgg gggccgggcg tgacgatccc cgacgcgccc
     1681 caggtgcagc gcggccaccc cagtttcgtg gtcatcccgc aagtgccagc gccgcagttc
     1741 tacatgtccg tgccggccaa caccggctcc tacaccctgg cgccgctgcc caccgacgcg
     1801 ctgcgcacgg ccaaagagat cgtcgatgcg ctgcagcagg cggtggcgca ggtcgatccg
     1861 gatcggctct acgtgaccgg gctctcgatg ggcggctacg gcgcctggga gatgatcgag
     1921 cgctatcccg gctacttcgc cgccgcggcg ccgatctgcg gcggcggcga tccgagcctg
     1981 gccgttcggc tggtcgacct ccccatctgg gccttccaca gcgccgacga cccgatcgtg
     2041 ccggtgcagg ccacgcgcga catgatcgcg gccatcgaag cggccggcgg ccagccgcgc
     2101 tacaccgagt tcaccgacgc cggtcacggc gcctgggtgc cggcctacgg catcggcggc
     2161 ctgccctcgc cggtcgccga cttctacgac tggctcttcg cccagcacaa gtgagtcctg
     2221 tactggcccg tatgcgtttc tgttttagaa agcacagttc aggtagcggc gcggtttacc
     2281 acgacgatac tcgctgctat actgacttct aagcccactt acacgcttcc gccataagga
     2341 gcagcgcatg aggagcgacg ccacctcggg aggagcaaga actctctcgg tgttcccgag
     2401 tgtcgctcgg attgcagtcg atgtgatcac gaaactcggg aaaggagcct gacgaagcga
     2461 gcttgccggc atcgtagatg agcaaggggt tcatctacgc gtcccagtgg tgctgagcgc
     2521 gaccgtaggg gtaccacaac ggggagggcg cgatggtgct tgactaccgc agccgcacgc
     2581 tgcttgagga gcattttgca gcgcgac
//
LOCUS       DASUBI010000032         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_953384, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000032 DASUBI010000000
VERSION     DASUBI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953384"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFU72_00530"
     CDS             <1..753
                     /locus_tag="VFU72_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5422001.1"
                     /translation="PFTALYELPPDEGIQWSQLLNQGIAEAAAAYPEEFVGFATVPLQ
                     DGVAAAAELERGAALGLRGVEILSSVNGVGLDDPSLEPFWETAAWLAMPILIHPSYVA
                     GTERLGPYYLRNLLGNPTETANAGARLIFGGVLERHPTLKVILAHGGGSLPQIIGRLQ
                     HGAEVRAECRHARSPREQLGNLYFDTIVFDPLILRHLVELAGAERVVLGTDYPFDMGE
                     TRPLEFVAGAGLADADVETILHAGERLLNGVA"
     gene            780..>2622
                     /locus_tag="VFU72_00535"
     CDS             780..>2622
                     /locus_tag="VFU72_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5422002.1"
                     /translation="MSSTLQPGRYVGKSIKRVNDPRLITGHGSFVDDIRLPGLGTLHA
                     AFVRSPYAHAEIGAIDAGPALQLPGVVAVVTGRELAELVRPQPAEGLPPHRVLRRYPL
                     VFDRARFAGDPVAVVVAEDPYTAADAAELVQVEYRPLPAVTNVEAALAGGAPLLYPEW
                     GDNLAWTWTVESGDVDGALANAAHVVEVRLVNQRLYPAFIEPRAVLVNYVPVTGELTA
                     WASSQTPHRLRGTLAEALGIPENLVRGIAPDVGGAFGAKGPVYPEYITVAALARTLGR
                     PVKWTESRSESFVGTTHGRDQIQRLRAAVDADGVLSALEVELLSNCGAYNAATVAQRT
                     GQMSVGPYFIQAIRTVVRGVMTNTSPTGAYRGAGRPEAAYMLERLTEAVARELKLDPA
                     EVRRRNFTPRDRFPYVAPTDLVYDSGDYQRTLDTALDRIGYQELRAEQERLRREGRLI
                     GIGIGFFCERAGPGWDSGEARVQPSGTIVVTTGISPHGQGEETTFAQIVADELGIPLE
                     DVTVKASDTAITPQGIGTFGSRGTSVGGSAVLLAVRQVKEKARRIAAQMLEAGEEDTE
                     FDDGRFQVRGAPDRSVTFKQVARVAYGMNGIPNGLEPGLDATSYFMPN"
BASE COUNT          372 a          974 c          923 g          353 t
ORIGIN      
        1 cccttcaccg cgctctacga gctgccgccc gacgagggca tccagtggtc ccagctcctc
       61 aaccagggga tcgccgaggc ggccgccgcc taccccgagg agttcgtcgg cttcgccacc
      121 gtgccgctgc aggacggcgt cgccgcggcg gccgagctgg agcggggggc cgcgctgggg
      181 ctgcgtgggg tggagatcct gagcagcgtc aatggcgtgg ggctcgacga tccctccctg
      241 gagcccttct gggagaccgc cgcctggctc gccatgccga tcctgatcca cccgagctac
      301 gtggccggca ccgagcgcct gggcccctac tacctgcgca atctgctggg caacccgacc
      361 gagacggcca acgccggggc gcgcctgatc ttcggcggcg tgctggagcg gcaccccacc
      421 ctgaaagtca tcctggcgca cggcggcggg tcactaccgc agatcatcgg gcgcctgcag
      481 cacggcgccg aggtgcgcgc cgaatgccgc cacgcccgct cgccgcgcga gcagctcggc
      541 aacctctact tcgacaccat cgtcttcgac ccgctcatcc tgcgccatct ggtcgagctg
      601 gccggcgccg agcgcgtcgt gctcggcacc gactacccct tcgacatggg cgagacgcgg
      661 ccgctcgagt tcgtcgcggg cgccgggctt gcggacgcgg acgtcgagac gatcctgcac
      721 gcgggcgagc gcctgctgaa cggcgtcgcc tgacgcgaaa atcctatgga ggagcttgga
      781 tgagcagcac gctacagccg gggcgctacg tcggcaagtc gatcaagcgg gtcaacgatc
      841 cccggctgat caccggccac ggcagcttcg tggacgatat ccgcctgccg ggcctgggca
      901 cgctccacgc cgccttcgtg cgcagcccct acgcccacgc cgagatcggc gccatcgacg
      961 ccggcccggc cttgcagctg ccgggcgtgg tggcggtcgt caccgggcgc gagctggccg
     1021 agctggtgcg gccccagccg gccgagggcc tgccgccgca ccgcgtgctg cgtcgctacc
     1081 cgctggtctt cgaccgggcg cgcttcgccg gtgatccggt ggccgtggtg gtggccgagg
     1141 atccctacac cgccgccgac gccgccgagc tggtccaggt ggagtacagg ccgttgccgg
     1201 cggttaccaa tgtcgaggcc gcgctggccg ggggcgcgcc gctcctctat ccggagtggg
     1261 gcgacaatct ggcctggacc tggaccgtcg agagcggcga tgtcgacggc gcgctggcca
     1321 acgccgcgca cgtggtcgag gtgcggctgg tcaaccaacg gctctacccg gccttcatcg
     1381 agccgcgcgc cgtgctggtc aactacgtgc cggtcaccgg cgagctgacc gcctgggcct
     1441 ccagccagac gccgcaccgg ctgcgcggca cgctggccga ggcgctcggc atccccgaga
     1501 acctggtgcg cggcatcgcg cccgatgtcg gcggggcctt cggcgccaag gggccggtct
     1561 accccgagta catcaccgtg gcggcgctgg cgcgcacgct cggccgcccg gtcaagtgga
     1621 ccgagtcgcg ctccgagagc ttcgtcggca ccacccacgg gcgcgatcag atccagcgcc
     1681 tgcgcgcggc cgtcgacgcc gacggcgtgc tgtcggcgct ggaggtggag ctgctctcca
     1741 actgcggcgc ctacaacgcc gccaccgtgg cccagcgcac cggccagatg tcggtcggcc
     1801 cctacttcat ccaggcgatc cgcaccgtcg tgcgcggggt gatgaccaac acctcgccca
     1861 ccggcgccta ccgcggcgcc gggcgccccg aggccgccta catgctggag cggctgaccg
     1921 aggccgtggc gcgtgaactg aagctcgacc ccgccgaggt gcgccggcgc aacttcaccc
     1981 cgcgcgaccg tttcccctac gtcgccccca ccgacctggt ctacgacagc ggcgactacc
     2041 agcgcacgct cgacaccgcg ctcgaccgca tcggctatca agaactgcgc gccgagcagg
     2101 agcggctgcg ccgcgaggga cggctgatcg gcatcggcat cggcttcttc tgcgagcgcg
     2161 ccgggccggg ctgggacagc ggcgaggcgc gcgtgcagcc cagcggcacc atcgtggtga
     2221 cgaccggcat ctcgccgcac ggccagggcg aggagacgac cttcgcccag atcgtggccg
     2281 acgagctggg catcccgctg gaggatgtta cggtgaaggc cagcgacacc gccattacgc
     2341 cccaggggat cggcaccttc ggcagccgcg gcacctcggt cggcggctcg gcggtgctgc
     2401 tggcggtgcg ccaggtgaag gagaaggcgc gccgtatcgc cgcccagatg ctggaggcgg
     2461 gcgaagagga taccgagttc gacgacggcc gcttccaggt gcgcggcgcg cccgaccgca
     2521 gcgtgacctt caagcaggtg gcgcgcgtgg cctacggcat gaacggcatc cccaacggcc
     2581 tggagccggg actggacgct accagctact tcatgcccaa cg
//
LOCUS       DASUBI010000033         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_884926, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000033 DASUBI010000000
VERSION     DASUBI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884926"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VFU72_00540"
     CDS             complement(<1..127)
                     /locus_tag="VFU72_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5422003.1"
                     /translation="MAKQKYERSKPHINIGTIGHVDHGKTTLTAAITKTLALQGGG"
     gene            complement(266..347)
                     /locus_tag="VFU72_00545"
     tRNA            complement(266..347)
                     /locus_tag="VFU72_00545"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Tyr,seq:gta)
     gene            complement(349..424)
                     /locus_tag="VFU72_00550"
     tRNA            complement(349..424)
                     /locus_tag="VFU72_00550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:Thr,seq:tgt)
     gene            complement(500..1582)
                     /locus_tag="VFU72_00555"
     CDS             complement(500..1582)
                     /locus_tag="VFU72_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEU5422004.1"
                     /translation="MAYQVTLIPGDGIGPEVSGAARRVLEATGIQFEWDTQDAGMTAL
                     EKTGDVLPDAVIESIQQNGIALKGPLTTPIGGGFRSVNVALRHALELYANLRPARTYE
                     GVQSTFHNINLVVVRENMEDLYMGVEFPEGGDETRQVIDFINARSPKKIMESSAISIK
                     AISPENSRRIVRFAFDYAIQNNRKLVTAVHKANIMKFTDGLFLKVAQEVASDYPQVEF
                     NDRIVDNMCMQLMQKPDLYDVMVMPNLYGDILSDLTSGMIGGLGVAPSANIGERGAVF
                     EAIHGSAPTHAGKNEANPTAMILSGVMLLRHIGEQQAAEAVDNAVAAVIKDGTHVTYD
                     LRPDRNREKAASTSEMTDAIIARLGR"
     gene            1746..2240
                     /locus_tag="VFU72_00560"
     CDS             1746..2240
                     /locus_tag="VFU72_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422005.1"
                     /translation="MPTKQEIEDAIRKGKERVQHTFGNLTDAELNRVVHEGDGGWTAK
                     EVLAHLAGRARGYERTVQTVQGQAPAQPAGGAFNVDQWNRDRINERIHKSRDELLQEF
                     HAVHDDLIAKVRGLTDPQLANTVSRPTGDISAGEALARAGGQHAINHSVEVEQALGKE
                     PPTS"
BASE COUNT          398 a          784 c          716 g          418 t
ORIGIN      
        1 tgcccccgcc ctgcaacgcc agcgtcttgg tgatcgccgc cgtcaaggtc gtcttgccgt
       61 ggtccacgtg cccgatcgtc ccaatgttga tgtgcggctt gctccgctcg tacttctgct
      121 tcgccacgct ctgtccctcc gaaagccaac tcggtaaccg ctaatgacgt ttggcgcgac
      181 tgccctcgac caggttcgaa ctggtgcgct cccggcgagc cgagggcacg acatgccaag
      241 cggcccatca tttcgacggg ccgggtgggg agggagggat tcgaaccccc gaagcattgc
      301 gcacctggtt tacagccagg cccctttggc cgctcgggaa cctccccctg gagccgacgg
      361 tgggactcga acccgcaacc ggctgtttac aaaacagctg ctctgccaat tgagctacgt
      421 cggcgcggcc cggtgcgacc accagacgcg taagtatagt ggcgcaccag cccctcgtca
      481 agcgccagcg gcgcccgcgt tagcgcccca gacgcgcgat gatcgcgtcc gtcatctcgc
      541 ttgtcgacgc cgctttttca cggttgcggt cgggccgcag gtcgtaggtg acatgcgtcc
      601 catccttgat caccgcggcc accgcgttgt cgaccgcctc ggccgcctgc tgctcgccga
      661 tgtggcgcag gagcatgacg ccgctcaaga tcatggccgt cggattggcc tcgttcttgc
      721 ccgcgtgcgt gggggcgctg ccgtggatcg cctcgaagac cgcgccacgc tccccgatat
      781 tcgcactcgg cgccacgccc agtccgccga tcatgcccga ggtcaggtcg ctcaagatgt
      841 ctccatagag attcggcatc accatcacgt cgtagaggtc gggcttttgc atcagctgca
      901 tgcacatgtt atcgacgatg cggtcgttga actcaacctg cggatagtcg gaggcgacct
      961 cctgcgctac cttgaggaag agcccgtcgg tgaatttcat aatgttggcc ttgtgcaccg
     1021 ccgtcaccag cttgcggtta ttctggatgg cgtagtcgaa cgcaaagcgc acaatccggc
     1081 gcgagttctc gggcgagatc gccttgatcg agatggccga cgactccatg attttcttgg
     1141 ggctgcgcgc gttgataaag tcgatcacct gacgggtctc gtctcccccc tcggggaact
     1201 caacccccat gtagaggtct tccatattct cgcgcacgac caccagattg atgttgtgga
     1261 aggtgctctg cacgccctcg taggtgcggg ccgggcgcaa gttagcgtac agctccagcg
     1321 cgtggcgcag cgccacgttg acgctgcgaa agccgccgcc gatcggcgtc gtcaacggcc
     1381 ccttgagcgc gatcccattc tgctggatcg actcgatgac cgcgtccggc agcacgtcgc
     1441 cggtcttctc caaggcggtc atgccggcgt cctgcgtgtc ccactcgaac tggatgccgg
     1501 tcgcctccag cacacgccgc gccgcgcccg atacctctgg tccaatcccg tcaccgggaa
     1561 tcaacgtcac ctgatacgcc atcgctccct cttttctccc aagacgcagg ccacttttca
     1621 catccggccc gattgtaggt ttgccgcgaa atcactgtca aaccgcgcgc caccgtggaa
     1681 tgccggcgcg gagtgcggcg ttcgactggg tgacgacatc cgccacggag aagggagcgg
     1741 gatcggtgcc gaccaagcag gagatcgagg acgccattcg caagggcaaa gagcgcgtcc
     1801 agcacacctt cggcaacctg actgacgcgg agctgaaccg cgtggtccac gagggtgatg
     1861 ggggctggac cgccaaagag gtattggcgc acctggccgg gcgagccagg ggctacgagc
     1921 gcaccgtcca gacggtgcag ggccaggctc ccgcccagcc cgccggcggc gccttcaacg
     1981 tcgaccagtg gaatcgcgac cggatcaacg agcgcattca caagtcgcgc gatgagctgc
     2041 tgcaggagtt ccacgccgtt cacgacgacc tcatcgcgaa ggttcgtggg ctgaccgatc
     2101 cccaactggc caacaccgtc tcgcgcccga ccggcgacat ctcggccggg gaggcattgg
     2161 cgcgcgcggg cggccagcac gcgatcaacc acagcgtcga ggtcgagcag gcgctgggca
     2221 aggagccacc gacttcctag ggtgctcggt gccgcgtgcg ggacggatcc ttcggctgcg
     2281 ctcagagcct gccctgagcc tgtcgaaggg atgaca
//
LOCUS       DASUBI010000034         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_190244, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000034 DASUBI010000000
VERSION     DASUBI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190244"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1026)
                     /locus_tag="VFU72_00565"
     CDS             complement(73..1026)
                     /locus_tag="VFU72_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5422006.1"
                     /translation="MKGITALVRSDRYRRHGISSHPENARRLVAIDERLAAAGLLDGR
                     LLREPAAATPADIELVHDAGYVREIAELAAAGGGYLDADTYVGPESYETALLAAGGAM
                     LGVDLVLSGAAPRAFVLPRPPGHHAERAQGMGFCIFNNVAIAARHALARHALGRVAIV
                     DWDVYHGNGTQDAFYDSDRVLFISLHQWPLYPGSGRPAELGVDAGYGFTLNLPLPPGS
                     DDTDYLTVFDEIVGPRLTEFAPELILVSAGYDAHRDDPLAMMSLTETGFAGMATRVRR
                     WADELSGGRLVGVLEGGYNLKALGAGVEATLRAWDQSEEQS"
     gene            complement(1079..1309)
                     /locus_tag="VFU72_00570"
     CDS             complement(1079..1309)
                     /locus_tag="VFU72_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU5422007.1"
                     /translation="MTKAFVLITTEIGKGDQVYDALKQLEGVTDVNIVAGTYDIVAVL
                     QAPETRDIGRIVMGPIQKLDGVMSTVTLMAVV"
     gene            complement(1306..2103)
                     /locus_tag="VFU72_00575"
     CDS             complement(1306..2103)
                     /locus_tag="VFU72_00575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5422008.1"
                     /translation="MSAPPDSPRTVALTAAREAGRILMEHLGRVEHVRYKGTIDLVTE
                     ADEASERRITEIIRARFPEDQILAEEGGASGSPHAERRWLVDPLDGTTNYAHAYPFFA
                     VSIACELRGELQLGIVLAPALGELFLAERGAGAFLNGERLAVSATDSLIRSMLTTGFH
                     YQPELIRRNLAAFERLVLSAQAVRRDGSAALDLCYVAAGRFDGYWELGLNPWDTAAGA
                     LLVREAGGALTDAEGQPYRLGAGSVVASNGHIHEQLLAGIRGEGGEP"
BASE COUNT          360 a          770 c          732 g          309 t
ORIGIN      
        1 ccggcacgcc aagacgcgtc agggcgttca ccacgctcgc caggttaccc gcgccatagt
       61 cgaccaccgc gatcacgact gctcctcgga ttgatcccag gcacgcagcg tcgcctcgac
      121 gcccgccccc agcgccttga gattgtagcc gccctcgagc acccccacca gacgcccgcc
      181 ggagagctca tcggcccagc gccgcacgcg cgtcgccatg ccggcgaaac ccgtttcggt
      241 caacgacatc atcgccagcg ggtcgtcgcg atgcgcgtcg tagccggctg agaccaagat
      301 cagttccggc gcgaactcgg tcagccgcgg accgacgatc tcgtcaaaga ccgtgagata
      361 gtccgtgtcg tcagatccag gcggcagcgg cagattgagc gtgaagccgt agccggcatc
      421 gacgcctaac tccgccggtc tcccgctacc ggggtagagc ggccactggt gcagcgagat
      481 gaagagcacc cggtcgctgt catagaaagc gtcctgcgtt ccgttgccgt ggtacacgtc
      541 ccaatcgacg atcgccaccc ggcccagcgc gtgccgcgcc agcgcgtggc gcgcggcgat
      601 ggcgacgttg ttgaagatgc agaaacccat gccttgcgca cgctcggcgt ggtggccggg
      661 cgggcgcggc aggacgaagg cgcgcggggc cgcaccggag agcaccagat caacgcccag
      721 catggcgccg ccggccgcca gcaaggcggt ttcataggac tcggggccga cgtaggtgtc
      781 ggcgtcgagg tagccgccgc cggccgcggc aagttccgcg atctcacgca cgtagccggc
      841 gtcgtggacc agctcgatat cagcaggcgt ggccgctgcc ggctcgcgca gcaggcgccc
      901 gtcgagcagc ccggctgccg ccagccgctc gtcgatcgcg accagccggc gcgcattttc
      961 gggatgcgaa ctgattccgt gtcgccggta gcggtcagag cgaaccagcg cggtgatgcc
     1021 cttcatggcg cgagtataaa acgaccgtta cgagcgcgcc aaaccgcggc cgtggaggtc
     1081 aaacgacggc catcagcgtc accgtgctca tgacgccgtc gagcttctgg atcggcccca
     1141 tcacgatgcg gccgatgtcg cgtgtctccg gcgcctgcag caccgccacg atgtcgtagg
     1201 tgccggccac gatgttgaca tccgtgacgc cttcgagctg tttgagcgca tcgtagacct
     1261 ggtcgccctt gccgatctcg gtggtgatca gcacgaaggc cttggtcatg gctcacctcc
     1321 ctcgccgcga atccccgcca gaagctgctc gtggatgtgc ccgttcgagg ccacgacaga
     1381 gcccgcgccc aggcggtacg gctggccctc ggcatcggtc agggcgccgc ccgcctcgcg
     1441 caccagcagc gcgcccgccg cggtgtccca cggattgaga ccaagctccc aatagccgtc
     1501 gaagcggccg gcggccacgt agcacaggtc gagcgcggcc gagccatcgc ggcgaaccgc
     1561 ctgcgcgctg agcaccaggc gttcaaacgc ggccaagttg cgccggatga gctcaggctg
     1621 gtagtggaag ccagtggtca gcatggagcg gatcaggctg tcggtcgcgg agacggccag
     1681 ccgctcgccg ttgaggaacg cgccggcgcc gcgctcggcc aggaacagct cccccagcgc
     1741 cggggccagc acgatcccca gctgcagctc gccacgcagc tcgcaggcga tcgagacggc
     1801 gaaaaaggga taggcgtggg cgtagttggt cgtgccgtcg agcggatcaa ccagccagcg
     1861 gcgctcggcg tgcggactgc cgctggcgcc gccctcctcg gccaggatct gatcctcggg
     1921 aaagcgagcg cggatgatct cggtgatgcg gcgctcgctg gcctcgtcag cctcggtgac
     1981 cagatcgatc gtccccttgt agcgcacatg ctccacccgg cccaggtgct ccatcaggat
     2041 gcgcccggcc tcgcgcgccg ccgtcagcgc gaccgtgcgg ggggagtcag gtggggcgct
     2101 cacgctccga tctccgcgcg agtcaccagc ggcagatgcg gcgcgacatc gagcgtcagc
     2161 ggagcgacct c
//
LOCUS       DASUBI010000035         8082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_162537, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000035 DASUBI010000000
VERSION     DASUBI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162537"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..849)
                     /locus_tag="VFU72_00580"
     CDS             complement(79..849)
                     /locus_tag="VFU72_00580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422009.1"
                     /translation="MPIDFQAPHNRESYTGREASPDWRSAMRGIVDPAGLRVADIGCG
                     GGIYTRAWAELGATQVTGVDFSEEQLAAARASSDDPRLSFRHGTALATGLPAQSVDLV
                     FERALLHHGIDRLAAVRESGRVLAPGGRLIIQDRTMADIALPGSPQHLRGYLFARFPR
                     LLAIEAARRPAPGELEAALGEAGFAAIGTSTLWETRSRYPSFDDLAAELRARVGRSIL
                     HELSDDELSELIAFLRQRLPEGEPLIDRDRWTIWSGRR"
     gene            complement(1037..1900)
                     /locus_tag="VFU72_00585"
     CDS             complement(1037..1900)
                     /locus_tag="VFU72_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422010.1"
                     /translation="MEKPQRPAPHLRPDPKILDREWAALVEADREQVERLREEPAGSE
                     HWELIAPHFVADPSRQGDAIVDALRAISGPGRVWLDVGAGAGRYALPLALSSRRVIAV
                     EQSPAMRAALLDSARQAGIENVELRAARWPEAADGLQADYSLMSHVGYDLPEINAFVD
                     ALESVTREHCVALLMDRVPNGGFERAWREIHGEDRVRLPAAREFIHLLLARGALPEVR
                     RFPRDGHEPTEELLRRDARRRLWLREGSDKDERLQALLDRALAAGPQDFQLPSTVALI
                     SWQPPRAVSAE"
     gene            complement(1909..3099)
                     /locus_tag="VFU72_00590"
     CDS             complement(1909..3099)
                     /locus_tag="VFU72_00590"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU5422011.1"
                     /translation="MPVGAKASLSPQEARQLHQQALVIDTQQPPITSGIVFTPGMRAR
                     LAELAQQGRTRAEAGPELERTLVHEIETSPEARDIYLDMWRQSGVTAACGTFSGPDRL
                     ETAFANAVRKIANAQAIATALEGQVAIARRADDIVRAQQDNTHAIVIDFQNTIAFGDE
                     LDRIDLFHGLGLRMVQLTYNLQNLVGDGCTEAYQGGLTYFGREVVKRLNELKILVDVS
                     HCSEQVGWDAMQVSAAPVIVSHSTSKAVAYHDRGKTDELARAIANQGGYFGVVVVPGF
                     IRDTPGASMADVVRQLAHLVDVCGIDQVGIGTDKAGPGPSTSSLVEYPASMPANLLGE
                     FNRGGFRNQEHRLTDEYNTEGFECFSDWPNITVALAEAGFNEDELRKILGLNYLRVFR
                     DVVG"
     gene            3283..4809
                     /locus_tag="VFU72_00595"
     CDS             3283..4809
                     /locus_tag="VFU72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5422012.1"
                     /translation="MTRRGWLLILLFLLFLGLPPGSRAAGSYGTPVPGRHIYDLTGLL
                     TPAQVRDLEQRAALVEQAGAPTIVYVRAEDVSYDATATDSQALMDAWDVESAPGAHDG
                     LVIFLNLNSLDPIHGHAVLYAGAKHYDGGKLPKSELQSIYDKSMAPRLKSNDLYGALA
                     AGLDAVAHQLQFGTAPSDRAAKIMKYARDPLEGLAVVGAGLVGWVAWKVRQSRLPRGI
                     DAPPTTSPPSDLSPAEAGAVVSGRISDRLLEATLLDLARQGALSIEPAKHHKAQVRLL
                     DARAAKSQLERFLWDRLAVEATPDHIVDSASLSTLPRQWSDARDILQQDLETKGLFDP
                     GAGSRRNPGYIAGGFALLAAFGLWQALGGPHDQWSALSIGLLALTGAVAIWMSYRLPS
                     TSAAGEKLAADWRGFRKGITSAAKGPIHAADLDLDAVFPYAVALGVAGSFDHRLKEAE
                     RAGYNPAWLSRTLVSDTPGQRTHYYTTWHAFHSSVTPSSSGSGSGGASSGGGSAGGSF
                     "
     gene            4938..6068
                     /gene="thiO"
                     /locus_tag="VFU72_00600"
     CDS             4938..6068
                     /gene="thiO"
                     /locus_tag="VFU72_00600"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEU5422013.1"
                     /translation="MGMQAFDVVIVGGGLLGAAVAYELARRGQRIAIVERGPIGGEAS
                     WASAGIISYPNRMNMTRERIEITRRSRERYPALVEQLEHETGVAIEYQRPGNLLVAVD
                     EAGAHAVNQLAEWQQGLGFRVEQLDPEAARRLEPALTEQVTAAWLAPDVGALRVQRLT
                     EALAMAASRRGAAIMSDVAVEEITHDGSRVTGVRTAQGFVPGGTVVLAAGAWTGNLSQ
                     SLGVKLPTRPVKGQLIAFAGAPTPPSHIISGRGGYILPRLDGTVTAAATEEEAGFDRR
                     VTGEGVAWLIGLAGTLCPTLPQGELVSSWTGLRPATADGEPIMGPVPGYEGLWVAAGH
                     FRTGAKEAPASAELVATSLLGGRIDPLLQPFLPSRFNAPESS"
     gene            complement(6126..6881)
                     /locus_tag="VFU72_00605"
     CDS             complement(6126..6881)
                     /locus_tag="VFU72_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422014.1"
                     /translation="MFRLATFAYWLDDQQRARELSEGLLANSRRTGNDEGIGKALYLL
                     ASQPPWREPSAAISLLEEATHCFQRAGNPHWAVLCQGSLAKRAMELGQLERATWLLEQ
                     AYATCRRLEHDWGAALALEWLGRVAVATGDNARAIMHLRESLRLLAEEQDTMQFALTL
                     IDITIPLARSGDTRQAVYLLASSEARLLEMGVTLMSWQQAIHQEVTTQARAVLGEPAM
                     QEIWEAGRQMSLADIAAQLGTDDPLTLVPKDPS"
     gene            complement(6891..>8082)
                     /locus_tag="VFU72_00610"
     CDS             complement(6891..>8082)
                     /locus_tag="VFU72_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422015.1"
                     /translation="TLTGLGGVGKTRLALEVASDLQESGTPSIGFISLATIHDPGQLA
                     TTILQALRGREDPRQTAEETLKQMLSGEARLLLLDNFEQIVEAAPLLSDLLAACSELK
                     LLVTSRVPLRLAGEHIFPLAPLASAAAVELFAARAHAIQHDAPSVSWDVSMLEAICSR
                     LDNLPLALELAAPHVAVLPPPALLQRLQQRLPLLTHGPRDAPERQQDLRATIAWSYDL
                     LPADVQALFRAVSVFAGGFSLDAAEAVARSPGAADASMLAHIRSLVEHNLIQPAPEPD
                     GTPRFLVLELVREYAREQLEAAAEAAAIQLAHAEYFARWASRQKTELYGHDRRAALRR
                     FDREQANLRAALAWCRAQDQPVIGLRLAAGASEYWRERGLGKEGYSGLRAFFPVVREA
                     PEDI"
BASE COUNT         1188 a         2761 c         2876 g         1257 t
ORIGIN      
        1 ccggcattcc gccgtcgtcg agtccctcac acatcgtagt ctctgcttcc tccgctgggc
       61 gcaccggctg gcaccgggtc agcgacggcc gctccagatc gtccagcggt cgcggtcgat
      121 gagcggctcc ccttccggca gccgctggcg caggaaggcg atcagctccg atagctcatc
      181 gtcgctgagt tcgtggagaa tcgagcggcc gacgcgagcg cgcagctcgg ccgccaggtc
      241 gtcgaagctg gggtagcggc tgcgcgtctc ccagagtgtg ctggtgccga tcgcggcaaa
      301 gccggcctcg cccagcgcgg cttccagctc gcccggcgcg ggccggcgcg ccgcctcgat
      361 cgccagcagg cgggggaagc gcgcgaagag atagccgcgc aggtgctgcg gcgacccggg
      421 gagggcgatg tcggccatgg tgcggtcctg gatgatcagc cgtccaccgg gcgccaggac
      481 gcgccctgac tcgcgcaccg ctgccagccg gtcgatgccg tggtgcagca gcgcgcgctc
      541 gaagacgaga tcgacggact gagcgggcaa gccggtcgcc agggcagtgc catgccgaaa
      601 actgaggcgc gggtcgtcgc tcgatgcccg cgcggcggcc agttgctcct cggagaaatc
      661 gacgccggtg acctgggtgg ctcccagctc ggcccaggcg cgcgtgtaga tgccgccgcc
      721 gcagccgatg tcggccacgc gtagcccggc agggtcgacg ataccgcgca tggccgaccg
      781 ccagtccggg gacgcctcac ggcccgtgta tgactcccgg ttgtgtgggg cctggaagtc
      841 gatgggcatg gtcgtcctcc cggggtgtct gaggcgggtg gtgactgaca acttcgtgtg
      901 gtggcgcgtg gggcacggat ccttcgacaa gctcaggatg acatgtttga ggaattgggg
      961 cgtacgagta cgtcggcacg tattcgtcag ccccctatta tccctgtcat gctgagcgca
     1021 gtcgaaggat ccgtccttac tcggcactca ccgctctcgg aggctgccaa ctgatcaacg
     1081 cgacggtgct ggggagctgg aagtcctggg gcccggcggc cagcgcccgg tcgagcagcg
     1141 cctgcaggcg ctcgtctttg tccgagccct cacgcagcca gagccggcgg cgggcgtctc
     1201 gtcgcagcag ttcctcggtg ggctcgtgcc cgtcgcgcgg gaagcggcgc acctccggca
     1261 gcgcgccacg cgccagcagc aggtgaatga actcgcgcgc ggccggcagg cgcacgcggt
     1321 cctcgccgtg gatctcgcgc caggcacgct cgaacccgcc gttcggcacg cggtccatca
     1381 agagggcgac gcagtgctcg cgcgtgacgc tctccagcgc gtcgacgaac gcgttgatct
     1441 ccggcaggtc gtagcccacg tgcgacatca agctgtagtc ggcctggagg ccgtcggcgg
     1501 cctcaggcca gcgcgcggcg cgcagctcga cgttctcgat ccccgcctgg cgggcggagt
     1561 cgagcagggc cgcgcgcatg gccgggctct gttcgaccgc gatcaccctc cggctgctga
     1621 gcgccagcgg cagggcgtag cgcccggcgc ccgcgccaac gtcgagccag acgcggccgg
     1681 gaccactgat ggcgcgcagc gcgtcgacga tggcgtcgcc ctgccgggag ggatcggcca
     1741 cgaagtgcgg cgcgatcagc tcccagtgct cgctgccagc cggctcctcg cgcagccgct
     1801 ccacctgctc gcggtcggcc tcgaccagcg cggcccattc ccggtcaagt atctttggat
     1861 cgggccgcag gtgcggcgcc ggccgctgag gcttctccac ggacactcct atcccaccac
     1921 gtcgcggaag acccgcagat agttcaaccc caggatcttg cgcagctcgt cctcgttgaa
     1981 gccggcttcg gccagcgcca cggtaatgtt cggccagtcg ctgaagcact caaagccttc
     2041 ggtgttgtac tcgtcggtca ggcggtgctc ctgattgcgg aagccgcccc gattgaattc
     2101 gcccagcagg ttggcgggca tcgaggcggg gtactcgacc agcgagctgg tgctgggacc
     2161 ggggccggcc ttatcggtgc cgatgccgac ctggtcgatg ccgcagacat cgaccaggtg
     2221 cgcgagctgg cgcaccacgt ccgccatcga ggcgcccggt gtatcccgga tgaagccagg
     2281 aacgaccacc acgccgaagt agccgccctg gttcgcgata gcgcgcgcca gttcatcggt
     2341 cttgccgcgg tcgtggtagg cgaccgcctt gctggtcgag tgcgagacga tgaccggagc
     2401 ggccgagacc tgcatggcgt cccagccgac ctgctcgctg cagtggctga catcgaccag
     2461 gattttcagc tcgttgaggc gcttgaccac ctcgcggccg aagtaggtca acccgccctg
     2521 gtaggcctcc gtgcagccgt cgccgaccag attctgcagg ttgtaggtga gctgtaccat
     2581 gcgcagaccc aacccatgga agagatcgat gcggtccagc tcatcgccga aggcgatggt
     2641 gttctgaaaa tcgatcacga tggcgtgcgt gttgtcctgc tgcgcccgca cgatgtcgtc
     2701 ggcgcggcgg gcgatcgcga cctggccctc aagggccgtg gcgatcgcct gggcgttggc
     2761 gatcttgcgc accgcgttgg cgaaggccgt ctccaaccgg tcggggccgg agaaggtgcc
     2821 gcaggcggcg gtgacgccgg attgccgcca catgtcgaga tagatgtcgc gcgcctcggg
     2881 tgacgtctcg atctcgtgca ccagcgttcg ttccagctct ggcccggcct ccgcgcgcgt
     2941 gcgcccctgc tgggccagct cggccaggcg ggcgcgcatg ccgggggtaa agacgatccc
     3001 ggaggtgatc ggcggttgct gggtgtcgat cactagcgcc tgctggtgga gttgccgcgc
     3061 ctcctgcggc gataagctag ccttggcgcc gaccggcatg gtgacctcct tcggtatgag
     3121 ctgctcgcct gggcgattgt agcagtatcc cgcgccccca cccgtagagg ctgacccctc
     3181 tcccaaagct gggtgaggag acgatggatt tcacgcccgc gagtcgccgt caggctacac
     3241 ttagggcgcg cgacagatgg atggttggga tggggggcgg gcatgacgcg gcgcggctgg
     3301 ctactgatcc tgctgttcct gctgttcctc ggtctgccgc ccggttcgcg ggcggcaggc
     3361 agctatggca cgcccgtgcc cggccggcat atctacgacc tgaccggcct gctgacgccc
     3421 gctcaggtgc gcgatctgga gcaacgcgcg gcgctggtcg agcaggcggg cgcgccgacg
     3481 atcgtttacg ttcgggcgga ggacgtcagc tacgacgcca cggcgaccga ctcacaggca
     3541 ttgatggatg cgtgggacgt cgaatcggcg ccaggcgcgc acgacggact ggtgatcttc
     3601 ctcaacctga actccctcga cccgatccac ggccacgccg tgctctacgc cggcgccaag
     3661 cactacgatg gcggcaagtt gcccaagtcc gagctgcagt cgatctacga caagtcgatg
     3721 gcgccgcggc tcaagagcaa cgatctctac ggcgcgctcg ccgccggcct cgacgccgtg
     3781 gcgcaccagc tccagttcgg cacggctccg tcggatcgcg cggcgaagat catgaagtac
     3841 gcgcgcgacc cgctggaagg gctggccgtc gtgggcgccg gactggtcgg ctgggtcgcc
     3901 tggaaggtgc ggcagtcgcg tctgccccgc ggcatcgacg cgccacccac cacgtcgccg
     3961 ccgtccgatc tctcgccagc cgaggccggg gcggtcgtct ccgggcggat cagcgaccgc
     4021 ttgctggagg ccacgctgct cgatctggcg cgccagggcg cattgagcat cgagcccgcc
     4081 aagcaccaca aggcgcaagt ccggctgctc gacgcgcgcg ccgccaagag ccagttggag
     4141 cggtttctct gggatcggct ggcggtcgag gcgacgccgg atcacatcgt cgacagcgcg
     4201 agcctgtcga cgctcccgcg ccagtggtcc gacgcgcgtg acattctcca gcaagacctg
     4261 gagacgaagg gtctcttcga tcctggcgcc ggctcccggc gtaaccccgg ctacatcgcc
     4321 ggcggcttcg cgctgctggc ggcgttcggc ctgtggcagg cgctgggtgg cccacacgat
     4381 cagtggagcg cgctgagcat cggcctgctg gcgctgaccg gggcggtggc catctggatg
     4441 agctaccgcc tgccgtccac cagcgcggcg ggggagaaac tggccgccga ctggcgcggt
     4501 tttcgcaagg gaatcacgtc ggccgccaag ggcccgattc atgccgccga tctggatctc
     4561 gacgccgtct tcccctatgc cgtggcgctc ggcgtggccg gctcgttcga tcaccggctc
     4621 aaggaagccg agcgcgccgg ctacaatccc gcctggctca gtcgcacgct ggtctccgac
     4681 acgccgggcc agcgcacgca ctactacacc acctggcacg ccttccactc ttcggtcact
     4741 ccgtcgagca gtggcagtgg cagcggcggc gcctcctccg gcggcggctc ggcgggcggg
     4801 agcttctagc ctcgtgaccc cgcacacagt agggggggcg tttggggacg ctggtcgatt
     4861 gaccggagcg tatctcgtgg tttgtaatgt gtgcgtcggt gccggcgaag ttgtgcggag
     4921 gtgagggcgg cgtggggatg ggcatgcagg cgttcgacgt ggtgatcgtc ggtggtgggc
     4981 tgctcggcgc ggcggtggcc tacgagctgg cccggcgcgg ccagcggatc gccatcgtcg
     5041 agcgtgggcc gatcggcggc gaggcttcct gggcctcggc ggggatcatc tcctacccta
     5101 atcgcatgaa catgacgcgt gagcgcatcg agatcacgcg tcgcagccgc gagcgctacc
     5161 ccgcgctggt cgagcaattg gagcacgaga ccggcgtggc gatcgaatac cagcggcccg
     5221 gcaacttgct ggtggcggtg gacgaggccg gggcgcacgc ggtcaaccaa ctggccgagt
     5281 ggcagcaggg gctcggtttt cgggtggagc agctcgatcc ggaggcggcg cgacggctgg
     5341 agccggccct gaccgaacag gtaaccgcgg cctggctcgc gcccgatgtc ggagccctgc
     5401 gggtgcagcg gttgaccgag gccctggcga tggcggccag ccgccgtgga gcggccatca
     5461 tgtcggacgt agccgtcgaa gagatcaccc acgacgggtc gcgcgtcacc ggcgtgcgca
     5521 cggcgcaggg gttcgtgccg ggcggcacgg tggtgctggc ggccggcgcc tggacgggca
     5581 acctgagcca atcactgggc gtcaagctgc cgacgcgccc ggtcaagggg cagttgatcg
     5641 ccttcgcggg cgcgcccacg ccgcctagcc acatcatctc cggccgtggc ggctacatcc
     5701 taccccggct cgacggcacc gtgactgccg cagccaccga ggaggaggcg ggcttcgacc
     5761 ggcgcgtgac cggcgagggg gtggcctggc tgatcggcct ggcgggcacg ctctgcccca
     5821 ccctgcccca gggtgagctg gtgtcctcct ggacgggcct gcgcccggcc acggccgacg
     5881 gggagccgat tatggggccg gtgcccggct atgagggact ctgggtggct gccggacact
     5941 tccgcacggg cgccaaggag gcgccggcca gcgccgagct ggtcgcgacc tcgctgctcg
     6001 gcgggcgcat cgacccgctg ctccaaccct tcctgcccag ccgcttcaac gcgcccgagt
     6061 catcgtagcg cgtccggtgc ctggccgcgg cgtccagccg cggcgacgag cgctgcccgg
     6121 tgtgcctagc tgggatcctt gggaaccaac gtgagcggat cgtcggtacc gagttgggca
     6181 gcgatgtcgg ccagagacat ctggcggccc gcttcccaaa tctcctgcat cgccggctca
     6241 cccagcacgg cccgcgcctg cgtcgtcacc tcttggtgaa tggcttgctg ccaggacatc
     6301 agcgtgacgc ccatctccag caggcgggcc tcggacgagg cgagtaggta gactgcctgt
     6361 cgcgtgtcgc cgctgcgcgc cagcgggatc gtgatatcga tgagcgtcag cgcgaattgc
     6421 atggtgtcct gctcctccgc gagaagcctc aggctctcgc gcaagtgcat gatcgccctc
     6481 gcgttgtcgc ccgtcgccac cgcgacacga ccgagccact cgagcgccag ggcggctccc
     6541 cagtcgtgct cgagacggcg acaggtggcg tacgcctgct ccagcagcca cgtggcacgt
     6601 tccaactggc ccagctccat cgcccgcttg gccagcgacc cctggcacag cacggcccag
     6661 tgtgggttgc cagcgcgctg gaaacagtgc gtggcttcct cgagcagcga gatcgcggcc
     6721 gacggctcgc gccacggcgg ctgtgatgcg aggaggtaca gcgccttgcc aatgccttca
     6781 tcgttgccgg tgcgccggga gttggccagc aacccctcac tcaactcccg cgcgcgctgt
     6841 tgatcgtcga gccagtaggc gaaggtggcc aggcggaaca tcgcctcgct ctagatatcc
     6901 tccggcgcct cgcgcacgac gggaaagaac gctcgcagcc cggagtagcc ttccttcccc
     6961 agcccgcgtt cacgccagta ttccgacgcg ccggccgcga ggcgcaggcc gatcacgggc
     7021 tgatcttgcg cccggcacca ggcgagcgcc gcgcgcaggt tggcctgctc gcgatcgaag
     7081 cgccggagcg cggcgcgccg gtcgtgtccg tagagttcgg tcttctgccg actggcccag
     7141 cgcgcgaagt actcggcgtg tgccagctgt atcgccgccg cctcggcggc ggcctcgagt
     7201 tgttcccgag cgtactcgcg caccaactcc aacacgagga atcgcggcgt gccatcaggc
     7261 tcgggcgccg gctggatgag gttgtgttca accaggctgc ggatgtgagc cagcatcgag
     7321 gcgtccgcgg cccccggcga gcgggccacc gcctccgccg cgtcgagcga gaagccgccg
     7381 gcgaagacgc tcacggcgcg gaagagcgcc tggacatcgg ccgggagcag gtcgtagctc
     7441 caggcaatgg tggcgcgcag atcctgctgc cgctccggcg cgtcgcgggg gccgtgggtc
     7501 aagagtggta accgctgttg caggcgctgc agcagcgccg gcggcggcag cacggcgacg
     7561 tggggcgcgg ccagctcgag cgcaagtggc aggttgtcga gccgcgagca aatggcttcc
     7621 aacatggaga catcccacga caccgacggc gcatcgtgct ggatcgcgtg cgcacgcgcg
     7681 gcgaagagct ccacggctgc cgccgaagcc agcggcgcga gtgggaagat atgctccccg
     7741 gcgaggcgga gcgggacgcg gctggtcacc agcagcttca gctcggagca agcggccagc
     7801 agatcgctca gcagaggagc cgcctcgacg atctgctcga agttatccag cagcagcagg
     7861 cgcgcctcgc cagacagcat ctgcttgagc gtctcctcgg cggtttgcct gggatcctca
     7921 cggccacgga gcgcctgcag gatggtcgtc gcgagttggc cgggatcgtg gatggtggcc
     7981 agcgagataa agccgatcga cggtgttccc gactcctgca ggtcgctggc gacctccagc
     8041 gccaggcggg tcttgccgac gccgcccagc ccggtgagcg tc
//
LOCUS       DASUBI010000036         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1102683, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000036 DASUBI010000000
VERSION     DASUBI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102683"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1814
                     /gene="gpmI"
                     /locus_tag="VFU72_00615"
     CDS             231..1814
                     /gene="gpmI"
                     /locus_tag="VFU72_00615"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5422016.1"
                     /translation="MADRPVVLVILDGWGLAPPDPGNAVRAARTPTMDRLSASYPYTT
                     LRASGLDVGLPAGQMGNSEVGHLNLGAGFVVYQWISRIDAAIADGSFFQNPALVGAVE
                     RAKSSGRNLHLLGLLGNGGVHASQQHLYALLRLAQQHGLERVFVHAFTDGRDTPPTSG
                     EGFMRELLEQMRALGVGRVASVSGRYYAMDRDQRWPRTAQAYDAVVCGKGAIAPDPLA
                     AITRSYTAEVTDEFILPTVIVGQDQRPLAPIQPGDSVIVYNFRSDRPRQLTQALSEPG
                     FAAFERCATPSNLAIITMTEYEKDLPVTVAFPPQDVEYPLARILAQAGLAQFHTAETE
                     KYAHVTYFFNGGREEPFPGEERRLVPSPKVATYDLQPEMSAAGVTEGVVGAIRSGRDA
                     FIIVNFANCDMVGHTGVFAAAVNAVETADRCLREVIEATLAAGGVALVTADHGNAEEM
                     LIPGTKEVWTAHTTNPVPFVLVAPVGDPLRHAKLRAGGRLADVAPTVLAVLGLPQPPV
                     MTGQSLIADVPTNDDDSRR"
BASE COUNT          325 a          718 c          654 g          334 t
ORIGIN      
        1 tgacaagggg gaggggatga ggtgtacgac gatgatcggc atggcccgac gccgatcctc
       61 ctcgtccgtc ccctactaac cctgtcattc agagcttgtc gaaggatccg acctcataca
      121 gcagccccga ggtcgacggg cacccaggtg ccccgaactc gcgccctccc cgtctgctag
      181 gatgtccgcg aggatgtgga cccgtagcgt cgatgggggt gggggtgcgc atggccgatc
      241 gacctgtcgt actggtgatt ctggacggct ggggactggc accacccgac cccggcaacg
      301 ccgtgcgcgc ggccagaacc ccgaccatgg accgtctgag cgcgtcgtac ccctacacca
      361 ccctgcgcgc ctccggtctc gacgtcggac taccggccgg gcagatgggc aactccgagg
      421 ttgggcacct taatctcggc gctggcttcg tggtctacca gtggatcagc cgcatcgacg
      481 ccgccatcgc ggacggctcc ttcttccaga accccgcgct cgtcggcgcg gttgagcgcg
      541 cgaagtcatc cggccgcaac ctgcacctgc tcggcttgct cggcaacggc ggcgtccacg
      601 cctcgcagca gcacctctac gcactcttgc gactggctca gcagcacggc ctggagcgcg
      661 ttttcgttca cgccttcacc gacggacgcg acacgccgcc gacctccgga gaggggttca
      721 tgcgcgagct gctggagcag atgcgcgcgc tcggggtggg tcgcgtcgcc agcgtcagcg
      781 gccgctacta cgccatggac cgcgaccagc gctggccgcg caccgcccag gcctacgacg
      841 ccgtcgtctg cgggaaggga gcaattgccc ccgatccgct ggccgccatc acgcgctcct
      901 ataccgcgga agtgaccgac gagttcatcc tgccgaccgt catcgtcggc caggaccagc
      961 ggccgctcgc gccgatccag ccgggcgact cggtgatcgt ctacaacttc cgctccgacc
     1021 gcccgcggca gctcacgcaa gcgctctccg aaccgggctt cgctgccttc gagcgctgcg
     1081 ccaccccctc gaacctggca atcattacca tgaccgaata cgagaaggac ctgccggtta
     1141 cggtggcctt cccaccgcag gacgtcgagt accccctggc gcgtatcctc gcgcaggccg
     1201 ggctggcgca gttccacacc gccgagaccg agaaatacgc ccatgtaacc tatttcttca
     1261 acggcggccg cgaggagccg ttccccggcg aggagcgccg cctggtgccc tcgcctaaag
     1321 tcgccaccta cgatctgcag ccggagatga gtgcggcggg cgtgaccgag ggcgtggtgg
     1381 gcgcgatccg ctccgggcgc gacgcgttca tcatcgtgaa tttcgcgaac tgcgacatgg
     1441 tgggccacac cggcgtcttc gcggcggcgg tcaacgcggt cgagactgcc gatcgctgcc
     1501 tgcgcgaggt gatcgaggcc actctggcag cgggcggcgt cgccctggtg accgctgacc
     1561 acggcaacgc tgaagagatg ctgatccccg gcacgaagga agtctggacc gcgcacacca
     1621 ccaacccggt ccccttcgtg ctggtcgcgc cggtaggcga cccgctgcgt cacgccaagc
     1681 tgcgcgcggg tggccgcctg gccgacgtcg cccccaccgt cctggccgtg ctcggcctcc
     1741 cgcagccccc ggtcatgacc ggccagtcgc tgatcgccga cgtgccgacc aacgatgacg
     1801 attcgcgccg ctgaggacgg atccttcgac tgcgctcagg atgacagggt aagagagggg
     1861 ctaacgagga aggtgggtct tgggcgacga ccttcctctt tcggtaggcc cctttctacc
     1921 cctgtcatcc tgagtggttc gaccgtcatg atttgtccgg ttcgaggacc ggatagagcc
     1981 gcttgagttt gatgcgggca tcggcggggg tgaactgcca atcgatggtg a
//
LOCUS       DASUBI010000037         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_598746, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000037 DASUBI010000000
VERSION     DASUBI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598746"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFU72_00620"
     CDS             <1..843
                     /locus_tag="VFU72_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5422017.1"
                     /translation="LLWPDTFNNHFYPETLHAAVEVLEAAGFQVLLPPRPLCCGRPLY
                     DFGMLDLARRLLDQILDALRPEIRAGVPLVGLEPSCVAVFRDELINLLPDDEDARRLS
                     QQSYLLSEFLEQQAPDVTWPRLERKAVVHVHCHHKSLSGTAALQRTLDRLGLDWTLLD
                     DSCCGLAGAFGYERGDHYTVSIKAGERVLLPAVRAAAPETVIIADGFSCREQIAQTTR
                     RRALHPAQVLQMALRAGTEGVPGITPERFAPTVVAKPGVGTAMLGTVLGAGAAWLWAR
                     GRQG"
     gene            849..1286
                     /locus_tag="VFU72_00625"
     CDS             849..1286
                     /locus_tag="VFU72_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU5422018.1"
                     /translation="MLLDRGRERTYAVVCDTGDEMIAVLTAFAKAEDLNASHFTAIGA
                     FSAATLGYFDYDTKEYLKIPITEQVEALALIGDIALDDGEPKVHAHVVVGLRDGTTRG
                     GHILQATVRPTLEITLSESPAHLRRTTDPETGLALINLGQRRG"
     gene            complement(1339..2283)
                     /locus_tag="VFU72_00630"
     CDS             complement(1339..2283)
                     /locus_tag="VFU72_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422019.1"
                     /translation="MATERTTRRPLKIGINLPSGEGLMAGQTPHWPDIRAMTVAAEQA
                     GYDSLWLPDHFLMKFSHGFTHPRGTWDCWTLLASMAAITDRVELGTLVSSTTFRNPAL
                     LARIADTIEDVSGGRLILGVGAGDSPYEHEAYGVPYDHRASRFEEAIQIIRGLLREGQ
                     VTFDGRFYQAHEAEIRPRGPRPQGPPIMIGARVTSPRMLRLCVEYADIWNEWVVFGNS
                     HPEAVRPQLQALDEACEAAGRDPKTLARSIAVMVDYSGRTEFRRGEQPLLGSPEELAD
                     ACRQFAAEGVSHLQIVFRPTTVETIERFAPVLGLLDQG"
     gene            2383..3153
                     /locus_tag="VFU72_00635"
     CDS             2383..3153
                     /locus_tag="VFU72_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEU5422020.1"
                     /translation="MERQETTRPMLAPPERRPWPLPEQPWVMRQSWRDLLFMHWPLPP
                     EQVRPLLPAALPLDTWEGEAWVSVVPFRMRGVTGRGLPAFPWLSSFPELNVRTYVTLG
                     GKPGVYFFSLDAGNILAVWAARLGYHLPYFHADMLVWREQEWITYCSHRVHRGARVGD
                     LLARYRPTGPVTEAPLGSLGAWLSDRYALYTVDRRGRPLRAEIDHATWPLQPAEVEIF
                     RDTLSTADGLALSGPPALLGFARRVDMHAWLPERVETG"
     gene            complement(3160..>3693)
                     /locus_tag="VFU72_00640"
     CDS             complement(3160..>3693)
                     /locus_tag="VFU72_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5422021.1"
                     /translation="ENETSPAGLTTSLLFRTPARRIVQRAIVYHSLHNRNHTAQRGWQ
                     RWRTTGARGQPMEYRVAGDDLEHRAFGHGGAVAALFHGGEHGLKAVSLMLGEVEPGDG
                     APLHRHPYEEVFVIHSGQGAYTIDGVTVEAGPSDIVLIPAHAPHSFINPGGTLLRHTA
                     VHAGPKIIIEWLEASVS"
BASE COUNT          521 a         1308 c         1299 g          565 t
ORIGIN      
        1 ctgctctggc cggatacctt caacaaccac ttctaccccg agacactcca cgccgcggtc
       61 gaggtgctgg aggcggccgg cttccaggtg ctgctgcccc cgcgtccgct ctgctgtggg
      121 cgcccgctgt atgacttcgg catgctcgat ctggcccggc gcctgctcga tcagatcctc
      181 gacgcgctgc gcccggagat ccgcgccggt gtgccgctgg tcggcctgga gccgagctgc
      241 gtggccgtct tccgcgacga gctgatcaac ctgctgcccg acgacgagga tgcgcggcgc
      301 ctgagccagc agagctacct gctgagcgaa tttctggagc agcaggcgcc ggacgtcacc
      361 tggccgcggc tggagcgcaa ggccgtcgtc cacgtgcact gccaccacaa gtcgctcagc
      421 ggcaccgccg cgctgcagcg gacgctcgac cggctcggcc tcgactggac gctgctcgac
      481 gacagttgct gcgggctcgc cggcgccttc ggctacgagc gcggcgacca ctacacggtc
      541 tcgatcaaag ccggcgagcg cgtcctcttg ccggccgtgc gcgccgccgc gccggagacg
      601 gtaatcatcg cggacggctt cagctgccgc gagcagatcg cccagacgac caggcggcgc
      661 gcgctgcacc cggcccaggt cttgcagatg gcgctgcgcg ccgggacgga gggagtgccc
      721 ggcatcacgc ccgagcgctt cgcgcccacg gtcgtggcga aacccggcgt cgggacggcc
      781 atgctgggca ccgtcctcgg cgcgggcgcg gcctggctct gggcgagagg gaggcaagga
      841 tgaagtcgat gctgctggat cgcgggcggg aacgaaccta cgccgtcgtc tgtgataccg
      901 gcgatgagat gatcgccgtg ctgaccgcgt tcgccaaagc cgaggatctc aacgccagcc
      961 acttcacggc gatcggggcc ttctcggcgg cgacgctggg ctatttcgac tacgatacca
     1021 aggagtatct caagattccg atcacggagc aggtcgaggc cctggcgctc atcggcgaca
     1081 tcgcgctgga tgacggcgag cccaaggtgc acgcgcacgt ggtggtcggc ctgcgggacg
     1141 gcaccacgcg cggcggccac atcctgcagg cgaccgtgcg ccccacgctg gagatcacgc
     1201 tgtccgagtc cccggcccac ctgagacgaa cgacggaccc cgagaccggt ctggcgttga
     1261 tcaatctcgg gcagcgccgc ggctgagcgc gcccggcgcc ctcgcgccct ggcaacgatc
     1321 gcttctgagc ggccggcgct agccctggtc gagcaggccg agcaccgggg cgaagcgctc
     1381 gatggtctct accgtggtcg ggcggaagac gatctgcaga tgcgagaccc cctcggcggc
     1441 gaactggcgg caggcgtcgg ccagctcctc gggcgagccg agcagcggct gctcgccgcg
     1501 ccggaactcg gtgcggccgg agtagtcgac catcaccgcg atgctgcgcg ccagtgtctt
     1561 cggatcgcgc ccggcggctt cgcaggcctc gtcgagcgcc tggagttgtg gccgcaccgc
     1621 ctcgggatgg ctgttgccga agacgaccca ctcgttccag atgtcggcgt actcgacgca
     1681 gaggcgcagc atgcgcgggc tggtgacgcg ggcgccgatc atgatcggcg ggccttgcgg
     1741 gcgcgggccg cgcgggcgaa tctcggcctc gtgcgcctgg tagaagcggc cgtcgaaggt
     1801 cacctgcccc tcgcgcaaga gcccccggat gatctggatc gcctcctcga agcggctggc
     1861 gcggtggtcg tagggcacgc cgtaggcctc gtgctcgtac ggtgagtcgc cggcgcccac
     1921 ccccaggatc agccgcccgc cgctgacgtc ctcgatcgtg tcggcgatgc gcgccagcag
     1981 cgccgggttg cggaaagtgg tgctgctgac cagcgtgcct agttcgacac ggtcggtgat
     2041 ggcggccatc gaggccaaga gcgtccagca gtcccaggtg ccgcgcgggt gggtgaaacc
     2101 gtgggagaac ttcatcagga agtggtcggg gagccagagc gagtcgtagc cggcctgctc
     2161 ggccgccacc gtcatggcgc ggatgtccgg ccagtggggc gtctggccgg ccatcagccc
     2221 ctcgcccgag ggaagattga tcccgatctt cagcggtcgc cgcgttgtcc gctccgtcgc
     2281 catcacgcgc tccttgcgta ctgtgtgccg gctgcgtacc ggcgtatcat cgcacggacc
     2341 gggcggggcg tgaaccgcgt ggcggccaag cagggagggt cgatggagcg acaagagaca
     2401 acgcggccga tgctggcgcc gcccgagcgg cggccctggc cgctgccgga gcagccctgg
     2461 gtgatgcgcc agtcgtggcg cgacctcctc ttcatgcatt ggccgctgcc gcccgagcag
     2521 gtgcgcccgc tgctgcccgc cgcgctgccg ctcgacacct gggagggcga agcctgggtc
     2581 agcgtggtgc ccttccggat gcggggcgtc accgggcgcg gcctgccggc cttcccctgg
     2641 ctctccagct tccccgagct gaacgtgcgc acctacgtca ccctgggcgg caagccgggc
     2701 gtctacttct tcagcctgga cgccggcaac atcctggcgg tctgggcggc gcgcctcggc
     2761 taccacctgc cctacttcca cgccgacatg ctggtctggc gagagcagga gtggatcacc
     2821 tactgctcgc accgcgtcca ccgcggcgcg cgcgtgggcg acttgctggc gcgctaccgc
     2881 ccgaccgggc cggtgaccga ggcgccgctc ggctcgctgg gcgcctggct gagcgaccgc
     2941 tacgcgctct acacggtgga ccgccgcggc cgcccgctgc gcgccgagat cgaccacgcc
     3001 acctggccgc tgcagcccgc cgaggtcgag atcttccgcg atacgctctc aaccgccgat
     3061 ggcctcgccc tctccggtcc gcccgcattg ctcggcttcg cccgccgcgt cgacatgcac
     3121 gcctggctgc cggagcgggt ggagacgggg taggcgcggt caactaaccg aagcctcaag
     3181 ccactcgatg atgatcttcg gcccggcgtg gaccgcggtg tgccgtagca gggtgccgcc
     3241 cgggttgatg aagctgtgcg gggcatgagc cgggatcaac acgatgtcgc tcgggccagc
     3301 ctcgaccgtg acgccgtcga tggtgtaggc gccctgcccc gaatggatca cgaagacttc
     3361 ctcgtaggga tggcgatgca gtggcgcgcc atcccccggt tctacctcgc ccagcatcag
     3421 cgagacggcc ttcagcccgt gctcgccgcc gtggaacagt gcggccaccg cgccgccgtg
     3481 gccaaaggcg cggtgctcca ggtcgtctcc cgcgacgcga tactccatcg gctgccccct
     3541 tgccccggtt gtccgccacc gttgccatcc gcgctgcgcc gtatgattgc gattgtggag
     3601 cgaatggtac acgatggccc gctgaacgat ccgtcgagcg ggcgtgcgga agagcaggct
     3661 tgtggtgaga ccggcaggcg acgtctcgtt ctc
//
LOCUS       DASUBI010000038         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_298390, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000038 DASUBI010000000
VERSION     DASUBI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298390"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFU72_00645"
     CDS             complement(<1..369)
                     /locus_tag="VFU72_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5422022.1"
                     /translation="MPVDPAFSLPGFQVGHWTHPSGATGCTVVICDQPALAVADVRGG
                     APGTRELAVLGDGRLVQRLDAVLLTGGSAFGLDAAGGAMRWLREQGRGFVTRSIPVPI
                     VAGAVIFDLDGPDPPWPDAAA"
     gene            complement(379..1152)
                     /locus_tag="VFU72_00650"
     CDS             complement(379..1152)
                     /locus_tag="VFU72_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5422023.1"
                     /translation="MRLTVLGGAAASPNPGQGCASYLVQSADEQLVLDCGPDTLSVLR
                     QHADFRQVRALLISHLHSDHTLDLVPYRYGLKYAPGPRGGRVPLWMPPGGRAFLGDLG
                     SVFALGSERDMPFFDEVFDVAEYHPGQQLQLGRFTVSFHETQHFIPAWAMSLSADGQT
                     LVYGADTGPYPPLAAFAAHADLIVLEATMPENQQQSGERAGHLSASQAGRLAAEAGAG
                     RLLLTHYWAEFDPEGLMREAASQFPGEVLLARPGLVVDL"
     gene            complement(1155..1793)
                     /locus_tag="VFU72_00655"
     CDS             complement(1155..1793)
                     /locus_tag="VFU72_00655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5422024.1"
                     /translation="MAEIFLVRHGQTASNIDFLLHGRTDVPLTALGEVQAAAVAMRLA
                     QIGGFATLYSSPLQRAARTAQAIGQRVNLTPVHVDALAELDFGDFEGLTLRAIEEQYP
                     ELFARILDSNDHDFRFPRGETRREFNQRVRRAFDDFAAQHQTERLIVVAHGGVIASVV
                     AQLSGGEINDWSRWLVHNCSITQLSLGGSGEFALQCWNEVGHLDTVTAQDRN"
     gene            complement(1786..2376)
                     /gene="nth"
                     /locus_tag="VFU72_00660"
     CDS             complement(1786..2376)
                     /gene="nth"
                     /locus_tag="VFU72_00660"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5422025.1"
                     /translation="MLDGLIGTILSQHTSDLNSERALQSLKARFTCWADVRDAPLEQV
                     IEAIRCGGLAEVKAPRIQQVLTIVLEQGWLDHLDEMPLAEAKQRLTSLPGVGPKTAAC
                     VLLFSCGRPALPVDTHVYRVASRLGLISPNTSAEAAHDQLERQLAPDEVYSFHVNMIR
                     LGREICHARNPECERCPLADVCVYYRNQRRSVSTHG"
BASE COUNT          459 a          868 c          911 g          401 t
ORIGIN      
        1 cgccgcggcg tccggccagg gcgggtcggg gccgtcaaga tcgaagatga ccgcgccggc
       61 gacgatcggg accgggatgc tgcgcgtgac gaaaccgcga ccctgctcac gcagccagcg
      121 catcgcgccg ccggcggcat cgagcccgaa ggcgctgccg ccggtgagca gcacggcatc
      181 gagtcgctgc accaaccggc catcgcccag cacggcgagt tcgcgtgtgc cgggcgcccc
      241 gccgcgcacg tcggccacgg ccagggcggg ttggtcgcag atgacgaccg tgcaaccggt
      301 cgcgccgctg gggtgggtcc agtgtccgac ctggaaacct ggcagcgaaa aagcagggtc
      361 aactggcatg ccgcaagctc acaagtcaac caccaagccg ggcctggcca ggagcacttc
      421 acccgggaac tgactcgcgg cttcccgcat caggccctct ggatcgaatt cggcccagta
      481 atgcgtcagc agcagccggc cggctccagc ctcggcggcg agccgccctg cctgcgatgc
      541 gctgaggtga cccgctcgct cgcccgactg ctgctggttt tcgggcatgg tcgcctccaa
      601 tacgatcagg tcggcatgtg ccgcgaacgc agcgaggggc ggatacggcc cggtgtcggc
      661 accgtagacg agcgtctgac catcggcgct caggctcatg gcccaggccg gaatgaagtg
      721 ctgtgtctcg tggaaggaga cggtgaagcg gcccagttgg agctgctgtc cggggtggta
      781 ctcggccacg tcgaagacct cgtcgaagaa cggcatgtcg cgctcgctac cgagcgcgaa
      841 gacgctgccc aggtcgccca ggaacgcccg tccgcccggc ggcatccaga gcggcacgcg
      901 gccgccacgc ggcccaggtg cgtacttgag gccatagcgg tagggaacga gatcgagcgt
      961 gtggtcggag tgcaggtggg agataagcaa cgcgcgcacc tgccgaaagt cggcgtgctg
     1021 gcgcaggact gacagcgtgt ccgggccaca atcgagcacg agctgctcgt cagcactctg
     1081 gacgaggtag cttgcacagc cctggccggg gttcgggctc gcggcggcgc cgccgagaac
     1141 ggtcagacgc atcgtcagtt ccgatcctgc gcggtcacgg tatcgagatg ccccacctca
     1201 ttccagcact gcagcgcgaa ttcgccgcta ccgcccagcg agagctgcgt gatgctgcag
     1261 ttatggacca gccagcgaga ccagtcgttg atttcgccgc ccgaaagctg agcgaccacc
     1321 gaggcgatga cgccaccgtg ggcaaccacg atcaagcgct cggtctggtg ctgcgcggca
     1381 aaatcgtcga acgcgcgccg cacgcgctgg ttgaactctc gccgcgtctc gcctcgggga
     1441 aagcggaaat cgtggtcgtt agaatccagg atgcgggcga acaactcggg atactgttcc
     1501 tcgatcgcgc gcagcgtcaa gccctcaaaa tcgccgaagt ccagctcggc gagcgcatcg
     1561 acatgcaccg gagtcagatt cacgcgctga ccaatggcct gcgcggtgcg ggcggcgcgc
     1621 tgcagcggac tggagtagag ggtggcaaag ccgccgatct gcgccaggcg catggcaacc
     1681 gcggctgcct gcacttcgcc cagcgcggtc aagggcacat cggtgcggcc gtgcaacagg
     1741 aaatcgatgt tgctggcggt ctggccgtgc cgtaccagga agatctcagc catgggtcga
     1801 cacactccgt cgctggttgc ggtagtagac acacacgtca gccagtgggc agcgctcaca
     1861 ctccggattg cgcgcgtggc agatctcgcg gccaagccgg atcatgttca cgtggaagct
     1921 gtagacttcg tccggcgcga gctgccgctc gagttgatcg tgcgccgctt cggcgctcgt
     1981 gttcggcgag atcaagccga ggcggctggc cacacggtag acgtgcgtat cgaccgggag
     2041 cgccggccgc ccgcaggaga agagcaggac acaggccgcc gtcttcggcc ccacaccggg
     2101 cagcgaggtc agccgttgct tggcttctgc cagcggcatc tcgtccagat ggtcgagcca
     2161 gccctgctct agcacgatgg tcagcacctg ctggatgcgc ggcgccttga cctcggccag
     2221 gccgccgcag cggatcgctt cgataacctg ctcgagcggc gcgtcgcgca cgtcggccca
     2281 gcaggtgaag cgcgccttga gcgactgaag cgcccgctcg gagttcaagt ccgacgtgtg
     2341 ctgcgagagg atcgtgccga tcaagccgtc gagcaccggc tgctttggcc ccggttcagg
     2401 ccggccatag aaggcttcca gccgctgccg tatttcggag ggcgtcgcca gcgtcgactc
     2461 ctttcgaagt ctagtctacc gccaggagcg gtcgcgcgga atgggaatgc acgcggtggg
     2521 tgacgagctg gtggggatcg gtgtgcggca cgaacgattg agcggaatcc tctgccatcc
     2581 cacctcgttg ccgggcaacg gccccatcgg tacgctcggc gccgaagcgc tggcgttcg
//
LOCUS       DASUBI010000039         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_516075, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000039 DASUBI010000000
VERSION     DASUBI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516075"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFU72_00665"
     CDS             complement(<1..787)
                     /locus_tag="VFU72_00665"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5422026.1"
                     /translation="MAGFPAPETMRPAITGRHYVAAAGHYLATMAAVRILEAGGNAID
                     AGVAAGLCTNVLQPDMTNVGGVAPIILYTAKDRKLRTISGLGSWPAAATPEYFVEHLG
                     GRIPGGVHHCVTPAAIDSWTTALRLFGSLSFAEVAAPAIALAEEGFAAYPFLCANLEA
                     DAERLAQWPSSAAIYLPNGRAPRPGERFVQADLAGLLRMLVEAEAGARHLGREAGIQA
                     ARDRFYQGDIAGRTARFFAEEGGFLTLDDLKAFHVEVEEPVGMS"
     gene            complement(835..2004)
                     /locus_tag="VFU72_00670"
     CDS             complement(835..2004)
                     /locus_tag="VFU72_00670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5422027.1"
                     /translation="MSIGSAYLRGARIVVIGAGAIGSVLTYRLAQAGAQVTLVERAFP
                     GSGTTGNSFAWVNSFGKTPREYHRLNASGLRAHQELARELDGEWLHAHGGLQWAYAAD
                     QDHVQHLRDTVKRLREWGYRVETLTPEQATRDLEPDLFIDPDTVEEVFYTPNEAWING
                     VGLCHGATSAAVRRYGATLVHDEVTGFGLAGGAIEHVRLAGGGSLPADAVINAAGPNA
                     ARVAALAGVELPVTRQPGCLIVTEPAPVALRAVIHAPEGIVRGDGGWRLLLHGEDFDH
                     WVESETPITIDHPFCQQAVERARAVLPGLAGIRAEGVRFGVRPMPRDGRSIVGFDPGV
                     TGLYHVATHSGVTLCATLGQLVTEDLNGEQPPELEPFRPTRFTDRAALVYTGTQE"
BASE COUNT          267 a          781 c          766 g          296 t
ORIGIN      
        1 agctcattcc caccggctcc tcgacctcga cgtgaaacgc cttgagatcg tccagcgtca
       61 ggaagccgcc ctcttcggcg aagaagcgcg ccgtccgccc cgcgatgtcg ccctggtaga
      121 agcggtcgcg tgcagcctgg atgccggcct cgcgccccag gtgccgcgcc cccgcctccg
      181 cctcgaccag catgcgcagc agcccggcga ggtccgcctg tacgaagcgc tcgcccggac
      241 ggggcgcgcg gccgttgggc aggtagatgg cggcgctgga gggccactgg gcgaggcgct
      301 cggcgtcggc ctcgaggttg gcgcagagga aggggtaggc cgcgaagccc tcctcggcca
      361 gcgcgatggc cggcgcggcg acctcggcga agctcaggct gccgaagagc ctgagcgccg
      421 tggtccagga gtcgatggcg gcgggcgtga cgcagtggtg gaccccgccc gggatgcgtc
      481 cgcccaagtg ctcgacgaag tactccggcg tggcggccgc cggccagctg cccagcccgc
      541 tgatggtgcg cagcttccgg tctttggcgg tgtagaggat gatcggcgcc acgccgccga
      601 cgttggtcat gtcgggctgc agcacgttgg tgcagagccc ggccgccacc ccggcgtcga
      661 tggcgttgcc gccggcctcc aggatgcgga cggcggccat cgtcgccaga tagtgcccgg
      721 cggcggccac gtagtggcgg ccggtgatcg ccggccgcat ggtctccggt gctgggaacc
      781 ccgccatcgc gcctgtctcc tctccaacgt cagctccggc gcccggccgc ctcgctactc
      841 ctgcgtgccg gtgtagacga gcgcggcgcg gtcggtgaag cgcgtgggcc ggaacggctc
      901 cagctccggc ggctgctcgc cgttcagatc ctcggtcacg agctggccca gggtcgcgca
      961 gagcgtcacc ccgctgtggg tggcgacgtg gtagaggccg gtgacgccgg ggtcgaagcc
     1021 gacgatcgag cggccatccc gcggcatcgg ccgcacgccg aagcgcacgc cctcggcgcg
     1081 gatgcccgcc agaccgggca ggaccgcccg cgcgcgttcg accgcctgct ggcagaaggg
     1141 gtggtcgatg gtgatcgggg tctcggactc gacccagtgg tcgaagtctt cgccgtgcaa
     1201 gagcagccgc cagccgccgt cgccgcggac gatgccctcc ggcgcgtgga tcaccgcgcg
     1261 cagcgcgacc ggcgccggct cggtcacgat caggcagccg ggctggcgcg tgaccggcag
     1321 ctcgacaccg gccagggccg cgacccgggc ggcgttcggt ccggccgcgt tgatcaccgc
     1381 gtcggccggg agcgagccgc caccggccag gcggacgtgc tcgatcgcgc cgccggcgag
     1441 gccgaagccg gtcacctcgt catggaccag agtggcgccg tagcgtcgga ccgccgcgct
     1501 ggtcgcgccg tggcagagcc ccaccccgtt gatccaggcc tcgttgggcg tgtagaagac
     1561 ctcctccacc gtgtcggggt cgatgaagag gtccggctcg agatcgcgcg tcgcctgctc
     1621 cggcgtcagc gtttcgacgc gatagcccca ctcgcgcagc cgcttgacgg tgtcgcgcag
     1681 gtgctgcacg tggtcctgat ccgccgcgta cgcccactgc agcccgccgt gggcgtggag
     1741 ccattccccg tccagctcgc gcgccagctc ctggtgcgcg cgcagcccgc tcgcgttgag
     1801 ccggtggtac tcacgcggcg tcttgccgaa ggagttgacc caggcgaagg agttgccggt
     1861 cgtgccgctg ccggggaagg cgcgctcgac cagcgtcacc tgtgctccgg cctgtgccag
     1921 ccggtaggtc agcaccgagc cgatcgcccc cgccccgatc accacgatgc gcgcgccgcg
     1981 caaatacgcc gaaccgatgc tcatggctgc cggtcctttc tcacctcgac tatcgcgccg
     2041 gtcgcccgct gccggtcgcg cggcaccagc cgcgctccgg ccagcggttt cgccggcccc
     2101 gcgtccgcct
//
LOCUS       DASUBI010000040         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_843766, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000040 DASUBI010000000
VERSION     DASUBI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843766"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFU72_00675"
     CDS             <1..912
                     /locus_tag="VFU72_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422028.1"
                     /translation="YMMGVTTHGTATARLRMAGPAGQHVLKVWRNYRGIPYLNPQQGP
                     FGKLVEPNTWLINVTDGPAAQQDIWADPAPQDTVRATPAVNDRGGASLTVSPDKGTVG
                     STITVRGAGFPANQPVKLTWATRTGQKITATGLLEGFDEQLRDLPAATAGPDGGFTVA
                     VPVPDDYAGYHRISAASGNGSASAEAYFSIYSSVVQFTDTAKVGSDVVIHLKGVGWTI
                     QGKTYAITYDNAYLGYACSFSTKGDIEVHLPAAGGPGLHVIDLYPAVYEGQDPTPNIY
                     SRPNLTYVENHPGGPLPAFHLTLAVAP"
     gene            complement(1402..2280)
                     /locus_tag="VFU72_00680"
     CDS             complement(1402..2280)
                     /locus_tag="VFU72_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5422029.1"
                     /translation="MWTLRALRLGMVLTSIVPAPVGYTLCRLVGLTLLCLNHEARGAV
                     LDNQRQVAPGAKRRTLYRHALGVFVTVVMNYYDLLRLSTVDRDRLLDLLEVQGQEHLD
                     GVFAADRGVIILGAHLGNFGVVAQYPGVLGRPAVLVVEHVHPEPLFQFMARLRSALGI
                     GVIPPGRRAVMPILRLLRDDGVLILAGDRDVLRQGMPVPFFGRLTSLPTGPVELAART
                     GTPLIPAYTVRLSSRRSLLRIDAPLELSHTGDREADLRENTERMARCLERMIASAPGQ
                     WAVLQRIWPEDARDPD"
     gene            complement(2290..>2557)
                     /locus_tag="VFU72_00685"
     CDS             complement(2290..>2557)
                     /locus_tag="VFU72_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422030.1"
                     /translation="ELSERVFFRAPARLNLPAAEAFPAWLATVILRPGNTTLHLELWP
                     VASPAELTARAEDAVRRFVEQWRCPRALWPAPAEDALPEYRGAG"
BASE COUNT          456 a          846 c          892 g          363 t
ORIGIN      
        1 tacatgatgg gggtgaccac gcacggcacc gccaccgccc ggctgcgcat ggccggacca
       61 gccggccagc acgtgctgaa ggtttggcgc aactaccgcg gcatccccta tctgaacccg
      121 cagcaaggcc cgtttggaaa actcgtcgag cccaatacct ggctgatcaa cgtcaccgac
      181 ggtcccgcgg cacagcaaga catctgggct gatccggcgc cccaggacac ggtgcgcgcc
      241 acgccggccg tcaacgaccg cggtggagcg tcgctgacgg tgagcccgga caaggggacg
      301 gtcggctcga ctatcacggt gcggggcgcg ggcttccccg ccaatcagcc ggtgaagctg
      361 acctgggcta cccgcacggg gcagaagatc accgccaccg gcctgctgga aggcttcgac
      421 gagcagttgc gcgatctgcc ggcggcgacc gcgggtccgg atgggggatt cacggtggct
      481 gtcccggtcc ccgacgacta cgccggctac catcgcatca gcgccgcgag cgggaatggg
      541 agtgcaagcg ccgaggccta tttctcgatc tattcctccg tggtccaatt caccgacacg
      601 gcgaaggttg gcagtgacgt cgtcatccat ctcaaagggg tcggctggac gatccagggg
      661 aagacgtacg ccatcaccta cgacaacgcc tatctgggct acgcctgcag cttcagcacc
      721 aagggcgata tcgaggttca tctcccggcc gcgggcggcc ccgggctgca cgtcatcgat
      781 ctgtacccgg cggtctacga gggccaggat ccgacgccga acatctactc gcgtccgaat
      841 ctcacctacg tcgagaacca tccgggcggc ccactgccgg cgttccatct gacgctggcg
      901 gtcgcgccct gatgctggac tggagtgagt gcaagcttga cggggcatgt cggacatcag
      961 gcggagcccc acgacgctga accctaaacg cggcacgagc gtgagggacc cgccaagaga
     1021 ggaaggcgag gcaaccgcga tgttcacgct actgggcatc atcgttgggc tcgtcatcgt
     1081 cgggtcgctc gcgctcgtcg tcttggacga agtgggcaaa gccaggcggc agcgctagat
     1141 cggatcaccg tgaaggccgg cagcaggcca gcagtgagag gaggggggca tgatcatact
     1201 gagcgttatc aaggcggata ttggcgggtc cgtcggccac ttgggcatgc atcccgacag
     1261 ggcgatggtc agaagcgcga gggaggggag gcctccgcct cactgcggtc tggatcgatc
     1321 agccgtgttc caaggcgtcg cgtggatcgg tgggatgaag cgtggtggga tgagcccggc
     1381 gcggcggttc ttcccagtgc atcagtcagg atcgcgcgca tcctccggcc agattcgttg
     1441 cagcaccgcc cactgcccgg gagcgctggc gatcatgcgc tccaagcagc gcgccatgcg
     1501 ctccgtattc tcgcgcaggt ccgcctcgcg atcgcccgtg tgcgacagct ccagcggcgc
     1561 gtcgatgcgc agcagcgagc ggcggctgga gaggcgcacg gtgtaggccg gaatgagcgg
     1621 tgtgccggtg cgcgctgcca gttcgaccgg acccgtgggg agcgaggtga gccggccgaa
     1681 gaagggcacc ggcatgccct ggcgcagcac gtcgcgatcg ccggccaaga tcaacacgcc
     1741 gtcgtcgcgc agcaggcgca ggatcggcat gaccgcccgc cgccccggcg ggatgacgcc
     1801 gatgccgagt gccgagcgca gccgggccat gaactgaaat aacggctcag gatgcacgtg
     1861 ctcgactacc agcaccgcgg gccggcccag tacaccgggg tactgcgcca ccacgccgaa
     1921 gttgcccagg tgggccccca ggatgatcac gccacgatcc gccgcgaaga ccccgtccag
     1981 atgctcctgt ccctggacct ccagcagatc gagcagccgg tcacggtcga cggtgctcag
     2041 gcgcagcagg tcgtaatagt tcatgacgac cgtcacgaag acgcccaacg catggcggta
     2101 gagcgtgcgt cgtttggcgc cgggagcgac ctggcgctgg ttgtcgagca ccgcgccacg
     2161 cgcctcgtga ttcaggcaga gaagcgtcag gccgaccagc cggcagaggg tgtagcccac
     2221 cggcgccggc acgattgacg tcaggaccat gcccagacgg agcgcgcgca gcgtccacat
     2281 cgccgcgcct cagccggcgc cgcggtattc ggggagcgcg tcctcggccg gcgcaggcca
     2341 gagcgcgcgc gggcagcgcc actgctccac gaagcgccgt accgcgtcct ccgcgcgagc
     2401 ggtgagctcg gccggcgacg caaccggcca cagctccagg tggagggtcg tattgcccgg
     2461 gcgtaggatc acggtggcga gccaggcggg gaacgcttcg gcggccggga ggttgagacg
     2521 tgcgggcgcg cgaaagaaca cgcgctcgct cagttcg
//
LOCUS       DASUBI010000041         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_53694, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000041 DASUBI010000000
VERSION     DASUBI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53694"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..691)
                     /locus_tag="VFU72_00690"
     CDS             complement(47..691)
                     /locus_tag="VFU72_00690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422031.1"
                     /translation="MDDPKRVIAGGYDRLSERYVEWAGGSVRDPARPRYLDLLERALP
                     AGAVVLDLGCATGALATARLARRFRVTGVDLSPRQIELARAAIPQARFICADMANVRF
                     PPASFDAVTSFYALNHLPRQELAPLLASIAAWLRPSGLFVASFPAGNSPGNVEPNWLG
                     VPMYFSGFDAETNVRLVEETGLRIEQATVETIEEDGRPVQFLWVVARRIGNESL"
     gene            complement(694..1527)
                     /locus_tag="VFU72_00695"
     CDS             complement(694..1527)
                     /locus_tag="VFU72_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422032.1"
                     /translation="MMPVEQMPASQPVLRPPPGEPRPVPPRRGRRLGVLPSLVVGALA
                     LLLWIVLSRSGLVSDFLLPPPEDVLRALWHAAADGLLWRYLRVTVVESLSGFALGAAL
                     ALPLGYLTARSRLAARALEPYIAASQALPAVAVAPLLILWLGFGLAPIAVLCALIVFF
                     PATVNTALGIRDIDPEVLAAARVDGAGAWALLWHIELPLALPTILAGLRTSLTLSITG
                     AVVGEFVVGDRGLGGLMTIARGNFDTPLVFATLLTLAILATIYYLVARLCELWARRAF
                     S"
     gene            1637..2179
                     /locus_tag="VFU72_00700"
     CDS             1637..2179
                     /locus_tag="VFU72_00700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5422033.1"
                     /translation="MRDGATPQMRIRTEEPGDQPAVYEVVRQAFGRDTESELVEGIRR
                     SPGWIPELSLVAERDGAIVGHILLSRFTIAGEVGTGDVPALVLAPLAVAPAMQNQGVG
                     SALVRAGLARAQAMGERLVVLVGHPTYYPRFGFRPARPLGIQLPYPIRDEAFMVCVLG
                     ESGQPPVRGTVVFPPHLRDV"
     gene            2275..>2715
                     /locus_tag="VFU72_00705"
     CDS             2275..>2715
                     /locus_tag="VFU72_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422034.1"
                     /translation="MSIVTPGKQPPTNPNWLLKRPQWIRALIGPGKPVYRLPWLILPR
                     VTIDALLGRRRDIRADAPGLIASMPVPPVIEGVEHIPAAGRCVILPNHYERPDGVWVG
                     WGVVAIMGALAARRPTLPPARWVMTSTWQDCYLGPRRIAPERLRW"
BASE COUNT          401 a          915 c         1034 g          365 t
ORIGIN      
        1 aaacccacgg gtagccatcc acgagggcag acacaggggt ccgcccctac agcgactcat
       61 tcccgatccg ccgcgccacc acccagagga actggaccgg gcggccgtcc tcttcgatgg
      121 tctcgaccgt cgcctgctcg atccgcaggc ccgtctcttc gacgagccgc acgttggtct
      181 cggcgtcgaa gccggagaag tacatcggca cgccgagcca gttgggctca acattgccgg
      241 gcgagttgcc ggccggaaag ctcgcgacga agaggccgct gggtcgcagc caggcggcga
      301 tcgaggccag gagcggcgcg agctcctggc gcggcaggtg attgagggcg tagaacgagg
      361 ttacggcgtc gaaggaggcg ggcgggaagc gcacgttagc catatcggcg cagatgaagc
      421 gggcctgggg gatcgcggcc cgcgccagtt cgatctggcg tggcgagagg tcgacgccgg
      481 tcacccgaaa gcgcctggcc agccgcgcgg tcgccagcgc gccggtggcg cagccgaggt
      541 cgagcacgac cgcgcccgcc ggcagcgctc gctccagcag gtccagatag cgcggccggg
      601 cgggatcgcg cacgctgccg ccggcccact cgacgtagcg ctcgctcagg cggtcgtagc
      661 cgccggcgat gacgcgcttg gggtcgtcca tagtcacgag aacgcgcgcc gggcccagag
      721 ctcgcagagc cgggccacca ggtagtagat cgtggccagg atggcgagcg tcaggagcgt
      781 ggcgaagacc agcggggtgt cgaagttgcc gcgcgcgatc gtcatcagcc cgcccagccc
      841 gcggtcgccg accacgaact cgcccaccac cgcgccggtg atcgagaggg tcaggctggt
      901 gcgcagcccg gccaggatgg tcggcagcgc cagcggcagc tcgatgtgcc agaggagcgc
      961 ccaggcgccg gccccatcga cgcgggccgc cgccagcacc tcggggtcga tgtcgcggat
     1021 gccgagcgcg gtgttgaccg tggccgggaa gaaaacgatc agggcgcaga ggacggcgat
     1081 cggcgccagc ccgaagccga gccagaggat cagcagcggc gccaccgcca ccgccggcag
     1141 cgcctgactg gcggcgatgt agggctccag cgccctggcg gccagccggc tgcgagcggt
     1201 gaggtagccg agcggcagcg cgagtgcggc tcccagtgcg aaaccgctca ggctctcgac
     1261 cacggtgacg cgcaggtagc gccagaggag gccgtcggcg gccgcgtgcc agagggcgcg
     1321 cagcacgtcc tccggtggcg gcagcaggaa gtcggagacc aggccggagc gcgagaggac
     1381 gatccagagc aggagggcca gcgccccgac gaccagcgag ggcagcacgc cgagccgccg
     1441 gccacgccgg ggtggtacgg ggcgtggttc gcccggcggc ggccgcagaa ccggctggct
     1501 ggcgggcatc tgctctacgg gcatcatgcg ctgcgctccc tgggcggcgg tcgcgtgcgc
     1561 cgcctccgct tgattatgga cgagcggcgc gccgctgccg aatcgcggcg gcgtgccgga
     1621 ggaaaggatg ggctggatgc gtgacggggc gacgccgcag atgcgtattc gcacggagga
     1681 gcccggcgac cagccggcgg tctacgaggt cgtgcggcag gcgttcgggc gcgataccga
     1741 gtcggagctg gtggagggga ttcgccgctc gcccggctgg atcccggagc tgtcgctggt
     1801 ggccgagcgc gacggagcca tcgtcggcca catcctgctc agccgtttta ccatcgcggg
     1861 ggaagttggc acgggcgacg tgccggcgct ggtgttggcg ccgctcgcgg tcgcgccggc
     1921 gatgcagaac cagggagtgg gcagcgcgct ggttcgggcg gggctggcgc gcgcgcaggc
     1981 gatgggtgaa cggctggtgg tcctggtcgg tcatcccacc tactacccac gcttcggctt
     2041 ccggccggcg cggccactcg ggatccagct gccctatccg attcgcgacg aggccttcat
     2101 ggtgtgcgtg ctcggcgagt cgggccagcc gccggtgcgc ggcacggtgg tcttcccgcc
     2161 gcatctccgc gatgtctgag tgagtcgatc gccgcgcggc gggagtatga ttggggttgc
     2221 tgttccacgc gtagtggccg cgccgatttg cgttgtttgt tgcgttagag gtgagtgtcc
     2281 attgtgaccc ccggcaagca gccgcccacg aacccaaact ggctcctgaa gcgcccgcag
     2341 tggatacgcg cgctgatcgg ccccggcaag ccggtctacc ggctgccctg gctgatactg
     2401 ccgcgcgtga cgatcgacgc actcctcggg cgcaggcgcg atattcgcgc ggatgcgccc
     2461 ggcctgatcg cctccatgcc ggtaccgccg gtgatcgagg gtgtcgagca catcccggcc
     2521 gccggacgct gcgtcatcct gcccaaccac tacgagcggc ccgacggcgt ctgggtcggc
     2581 tggggcgtgg tggcgatcat gggggcgctc gctgcgcgcc gcccgactct gccgcccgcg
     2641 cgctgggtca tgaccagcac ctggcaggac tgttatctcg gcccgcggcg catcgctccc
     2701 gagcggctgc gctgg
//
LOCUS       DASUBI010000042         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1293334, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000042 DASUBI010000000
VERSION     DASUBI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293334"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="VFU72_00710"
     CDS             <1..1792
                     /locus_tag="VFU72_00710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5422035.1"
                     /translation="RRRRAVVPCLQDHAELSVHLVPHYPMTDQLACRTLSLSDPIFEQ
                     GEYVMAEAGARQQTTTADATTEPGVDFDVVVVGAGLAGLYMLYRLRELGLSVRVFEEG
                     DGVGGTWYWNRYPGARCDTESMEYSYSFSDAMVQEWHWTERYPAQPEILRYINHVADR
                     FDLRRDIQFETRVTAARYDAADNRWLLVTDRGQQLTARYCVMAVGCLSAAQVPAIPGL
                     ADFRGDWYHTGNWPHAGVDFSGKRVGVIGTGASGIQSIPVIARQATHLYVFQRTANYS
                     VPSRNGPLDSGYEQRLKAGFAGYRQIARESAGGFLFRHNEQSALEATPEEREEEFERR
                     WAHGGLGFRSAFADLGTDLAANETAAEFVRAKIRAIVRDPETAERLTPRDHPLGAKRL
                     PLDSGYYETFNRDNVTLVDIRRAPIEAITPTGLRTSEADYALDSIVFATGFDAMTGPL
                     FRIDIRGRDGRALREKWAEGPRAYLGLASAGFPNLFLITGPGSPSVLSNMIVSIEQHV
                     EWIADCLAYLVGHGLGRIEAEAAAEDGWVEHVEEVGNSTLYPLANSWYVGANIPGKPR
                     VFMPYIGGVGAYRRICDEVAAEGYRGFSLA"
     gene            complement(2038..2685)
                     /locus_tag="VFU72_00715"
     CDS             complement(2038..2685)
                     /locus_tag="VFU72_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5422036.1"
                     /translation="MKTMRGEIERYWRDLAKGMRLMPFAALEAAAALLLDCHQRGATI
                     FLAGNGGSAATASHFACDLAKGARAVGVPPFRVVALTDNVPLMTAWGNDTSYERVFAE
                     QLATLARPGDVLVAISASGNSPNILLATRAAKTSGAATLALTGRTGGKLGALADLAVR
                     VPLDAIEQVEDAHVVIAHSLCVTLRERLGSVEATPRAGDWRVEEVLAELVADLDV"
     gene            complement(2712..>3537)
                     /locus_tag="VFU72_00720"
     CDS             complement(2712..>3537)
                     /locus_tag="VFU72_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422037.1"
                     /translation="LTLPKAAVERFAAQGLLEQGVDLFLASEVPPGTGLGSSSAMTVA
                     LVAALGAYLGLSFAPAELAEEASRLEIERLAMPIGKQDQYASAFGGLNALTFRDTGTE
                     VRPLALAGGQRQALARRLLLFSTGSSRHSAEILRQQRSDTRRKPAVTAALHQLKELAA
                     AMQAALEGGALDEFGALLDVAWQEKKRLSGKISNAGIDTAYQAARAAGALGGKITGAG
                     GGGFLLLYCPPRRQSAVRAVMGELGLCELTFDLSRTGVEVVSGAVVDELPVPVPGD"
BASE COUNT          522 a         1277 c         1218 g          520 t
ORIGIN      
        1 gaggcggcgc cgggcggtgg tgccgtgtct ccaggatcat gctgagctgt ctgtccatct
       61 cgtcccccac taccccatga ccgatcaact cgcgtgccgt acactgagcc tgtctgatcc
      121 catctttgag cagggagagt acgtgatggc cgaagcgggg gcgcgacagc agacgacgac
      181 agccgatgcc accacggagc cgggtgttga cttcgacgtg gtcgtggtcg gcgccgggct
      241 ggccgggctc tacatgctct accgcctgcg cgagctgggg ctctcggtgc gggtcttcga
      301 ggagggcgac ggcgtcggcg gcacctggta ctggaaccgc taccccggcg cccgctgcga
      361 caccgagagc atggagtact cctactcctt ctcggatgcg atggtgcagg agtggcactg
      421 gaccgagcgc taccccgccc agcccgagat cctgcgctac atcaaccacg tcgccgaccg
      481 cttcgacctg cgccgcgaca tccagttcga gacgcgcgtc accgccgccc gctatgacgc
      541 ggcggacaac cgctggctgc ttgtgaccga ccgcggccag cagctcacgg cgcgctactg
      601 cgtcatggcc gtgggctgcc tctccgcggc gcaggtgccg gccatccccg gcctcgccga
      661 cttccgcggc gactggtacc acaccggcaa ctggccgcac gcgggcgtcg acttcagcgg
      721 gaagcgcgtc ggcgtgatcg gcaccggcgc ctccggcatc cagtccatcc cggtcatcgc
      781 ccggcaggcg acgcatcttt atgtcttcca gcggaccgcc aactactcgg tgccctcgcg
      841 caacggcccg ctcgattccg ggtacgagca gcggctgaaa gccggcttcg ccggctaccg
      901 gcagatcgcg cgcgagtcgg ccgggggctt cctcttccgg cacaacgagc aatcggcgct
      961 ggaggcgacg ccggaggagc gcgaagaaga gttcgagcgg cgctgggccc acggcgggct
     1021 gggcttccgc tcggcctttg ccgacctcgg cacggacctg gcggccaacg agaccgccgc
     1081 cgagttcgtg cgtgccaaga tccgcgcgat cgtgcgcgac ccggagacgg ccgagcgcct
     1141 gacgccgcgc gatcacccgc tgggcgccaa gcgcctgccg ctcgactccg gctactacga
     1201 gaccttcaac cgcgacaacg tcaccctggt cgatatccgc cgggcgccga tcgaggcgat
     1261 cacacctaca gggctgcgca ccagtgaggc ggactacgcg ctcgatagca tcgtcttcgc
     1321 caccggcttc gacgccatga ccggcccgct cttccgcatc gatatccgtg gccgtgacgg
     1381 ccgcgcgctg cgggagaagt gggccgaggg gccgcgcgcc tacctcggac tggcctcggc
     1441 cggcttcccc aacctcttct tgatcaccgg ccccggcagt ccctcggtgc tcagcaacat
     1501 gatcgtctcg atcgagcagc acgtagagtg gatcgcggac tgcctggcat atctggtggg
     1561 gcacgggctg ggccgcatcg aggcggaggc cgcggcggag gacggctggg tggagcacgt
     1621 cgaagaggtc ggcaacagca cgctctaccc gctggcgaac tcctggtacg tcggggccaa
     1681 catccccggc aagccgcgcg tcttcatgcc ctacatcggc ggcgtcggcg cctaccgccg
     1741 catctgcgac gaggtcgcgg ccgagggcta ccggggcttc agcctcgcct gagacgcggt
     1801 gtgaggaagc gtgggaacga tgcggtggct atcgaggagc atgcgtgccc tcgaggccgg
     1861 atccttcgac tgcgctcagg atgacagggt gagtaggggg cctatcggac gaggagaata
     1921 gtcgtccaag gcccatcctc gtcgtcagcc tcctaataac cctgtcatcc tgagcgcagt
     1981 cgaaggatcc gtcctggacc acatcccaac gtcttcgtca gcctcgcctg agacgggtca
     2041 gacgtccagg tcggccacca gctcggccag cacctcctcg acgcgccagt cgccggctcg
     2101 tggggtggcc tcgacgctgc ccagccgctc gcgcagcgtg acgcagaggc tgtgcgcgat
     2161 gacgacgtgc gcgtcctcga cctgctcgat ggcgtcgagc ggcacgcgca ccgcgagatc
     2221 ggccagcgcg cccagcttgc cccccgtgcg gccggttagt gcgagggtgg cggcgccgga
     2281 ggtcttcgcg gcccgtgtcg ccagcaggat attgggcgag ttgccactgg cgctgatggc
     2341 gaccagcacg tcgccggggc gcgccaaggt cgccaactgc tcggcgaaga cgcgctcgta
     2401 gctggtgtcg ttgccccagg cagtcatgag cggcacgttg tcggtcaagg ccaccacccg
     2461 gaagggcggc acgcccacgg cgcgcgcacc cttggccagg tcgcaggcga agtgcgaggc
     2521 ggtggcggcg ctgccgccgt tgccggccag gaagatggtg gcgccgcgct ggtggcagtc
     2581 gagcagcagg gccgccgccg cctccagcgc cgcgaagggc atcagtcgca ttcctttggc
     2641 cagatcgcgc cagtagcgtt cgatctcgcc gcgcatggtc ttcatcgggt actcctcgtc
     2701 actcgcgccg gctagtcgcc gggcaccggc accggcagct cgtccacgac cgcgccgctc
     2761 accacttcaa ctcccgtgcg gctaaggtcg aaggtcagct cacacaggcc cagctccccc
     2821 atcaccgcgc gcacggcgct ctgccggcgc ggcgggcagt agaggaggag gaagccgccg
     2881 cccccggcgc cggtgatctt gccgcccagc gccccagcgg cgcgcgccgc ctgataggcc
     2941 gtgtcgatgc cggcgttcga gatcttgccc gagagccgct tcttctcctg ccaggccacg
     3001 tcgagcagcg cgccgaactc gtccagcgcg ccgccctcca gcgcggcctg catggcggcc
     3061 gccagctcct tcaactggtg cagcgccgcg gtcacggccg gcttgcggcg ggtgtcggac
     3121 cgctgctggc gcaggatctc ggcggagtgg cggctgctgc cggtcgagaa gaggagcagc
     3181 cgccgcgcca gcgcctgccg ctggccgccc gcgagcgcca gcgggcgcac ctcggtgccg
     3241 gtgtccctga aggtcagtgc gttcaagccg ccgaaggcgc tggcgtactg atcctgcttg
     3301 ccgatcggca tggcgagccg ctcgatctcc agccggctcg cctcctcggc cagctcggcc
     3361 ggcgcgaacg acagccccag gtaggcgccg agcgcggcga ccagcgccac ggtcatggcg
     3421 ctggaggagc cgagcccggt gccgggcggc acctccgagg ccaggaagag gtcgacgccc
     3481 tgctccagca gcccctgggc ggcgaaacgc tcgaccgccg ccttgggcag cgtcagc
//
LOCUS       DASUBI010000043         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_393723, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000043 DASUBI010000000
VERSION     DASUBI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393723"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFU72_00725"
     CDS             complement(<1..184)
                     /locus_tag="VFU72_00725"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5422038.1"
                     /translation="MAELTMSRWFMDKSGAESDGGMVVAKQDQAALAGARMLAEGGNA
                     VDAAIAAGFVMNVMEPY"
     gene            complement(547..759)
                     /locus_tag="VFU72_00730"
     CDS             complement(547..759)
                     /locus_tag="VFU72_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422039.1"
                     /translation="MDPMIAEMMIRQRHAELFECGRQSRPGYERRDANWLRLDKRAEH
                     ASGAGLLSLPARLFARLSAPRHAAQW"
     gene            complement(984..1295)
                     /locus_tag="VFU72_00735"
     CDS             complement(984..1295)
                     /locus_tag="VFU72_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422040.1"
                     /translation="MTMKVETMEDVAAVRRWLSDNVREGNGLARRFLRVLDRAAPGTE
                     IATARLVVAAIFGIDSQSDDLLRRWYAAAGAPYGDSDEGRRRWAAEQAAAERSRRDRL
                     N"
     gene            complement(1336..3681)
                     /locus_tag="VFU72_00740"
     CDS             complement(1336..3681)
                     /locus_tag="VFU72_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU5422041.1"
                     /translation="MATTQLDINSVLPAEALTCQHCLARFEATLQASPGVNQVALNPA
                     HGVAMVHFNPTLTTADTLVEQARQEGRRLGEDYAHRVLPIEGMDCADCAKTLQRAVSG
                     LAGVRDASVNFLGARMAVEYVPGETNIEAITRRVAEAGYRVSEPAAEAEQPRQGWRLP
                     GDRATRLTASGAALVVAGLAASLAGAGSLGTALYALAALIAGVPVARRGWASLRLGHQ
                     LDINVLMIIAVLGAAALGDWLEAATVVVLFSIGESLEGYTVERARRSIRSLLSLAPSE
                     AVLRTAHGEQRVPASAVAPGDLVIVRPGERVPADGVVASGVSAVDQAPITGESVPVEK
                     APGDEVFAGTVNGAGVLEVRASRPASDSTLARIIHLVEEAQARRAPTQRLVDAFARRY
                     TPAVVAGAVLLALAPPLLFGGDWSSWLYRALVLLVVACPCALVISTPVTIVAALATAA
                     RRGVLIKGGAHLETAGQLRALAFDKTGTLTEGRLAVTAVTPVDGATADELLALAAAAE
                     RYSEHPLGAAIVRAAEARGLTPLPASAVRAQPGGGIEARVDGALVRVGTRAFAGNGDQ
                     LLEERLAELEAGGQTAVVVNAGGRTIGLLGLADQLRPEARAAVAALKQAGIDTTVMLT
                     GDRLPVAEAVARAAGVDAVEAELLPEQKVTAVERLLGRYGRVGMVGDGVNDAPALARA
                     SLGIAMGAAGSHAALETADVALLSDDLSRLAGVIRLSRAARRVIIENVSLALGLKLIV
                     LVLAVAGAATLWEAVFADVGASLLVTANGMRLLRASSLRTD"
     gene            complement(3681..4106)
                     /locus_tag="VFU72_00745"
     CDS             complement(3681..4106)
                     /locus_tag="VFU72_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5422042.1"
                     /translation="MARPRKRDQIREPAQLETCADPIVHLDAVQRSRARLPEAAAIAE
                     LSALFAAVGDPTRLRLVAALAGAELCVCDLAATCGLSQSAVSHHLRLLRELGLVRARR
                     DGRLVYYALDDAHVAALFDQGLDHVRHRLARNEERGKTA"
BASE COUNT          627 a         1547 c         1525 g          590 t
ORIGIN      
        1 tgtagggttc catcacgttc atcacgaagc cggcagcgat ggcggcgtcg accgcgttcc
       61 ccccctcggc cagcatgcgg gcgccggcca gcgcggcctg gtcctgcttg gcgactacca
      121 tgccgccgtc ggattcggcg ccgctcttgt ccatgaacca gcgcgacatc gtgagctcgg
      181 ccatgcgcgt gctgctcccc tctcgccgta cccgctcgac gcggccatgc taccccggat
      241 tgggcgttgc gtcatgcggg gcgggcaagt ggctgacgac aagcgccgga cgtgtgtgga
      301 ggctgtaccc tgcgactgcg ctcaagatga cagagttggc aaggggcgga cgacgaggaa
      361 tagcctggtc cgaaaggcct caacgaaaca ccgtcatgct gggcgcagtc gcaggatccg
      421 tcctcgatgg caacaacata tcccggtgtc ctcagacgcc gggaaacgaa aaacccgctc
      481 ggttcagacc gagcgggttt cggtgacggt cgcgtctgcg acagtcggtg ggcgtgtctg
      541 tcgcggctac cattgcgcgg cgtggcgcgg agcgctcagg cgtgcgaaca gccgcgccgg
      601 caggctcagt aggccagcgc cgctggcgtg ctcggcgcgc ttgtcgagcc gcagccagtt
      661 cgcgtcgcgc cgctcgtagc ccgggcggct ctgccggccg cactcgaaga gctcggcgtg
      721 gcgctggcga atcatcatct cggcgatcat cgggtccaag ggacacctcc ttattacatt
      781 ctctatatta ggcgtcccga gattccgcag cgccgctcgg ccggacatcc atctcctgta
      841 ccagtggaca gccgttctac tgcccaatct gccatcaagg ttcaggttcc gaatcgtcaa
      901 aatcgtgcgg aaaatcagcg gtttcggagg ccacgcacga caaagtagat tgaagaagca
      961 agaaaagggc ggctcggccc cgctcagttg aggcggtcgc ggcggctgcg ttcggcggcg
     1021 gcctgctcgg cggcccagcg gcgtcggccc tcgtcgctgt cgccgtaggg cgcgcccgcc
     1081 gccgcgtacc agcggcgcag caggtcgtcc gactgggagt cgatgccgaa gatggcggcg
     1141 accaccagcc gcgcggtggc gatctcggtg ccgggcgcgg cgcggtccag cacgcgcagg
     1201 aagcgccggg ccagcccgtt cccctcgcgc acgttgtcgg agagccagcg gcgcacggcc
     1261 gccacgtcct ccatcgtctc gaccttcatc gtcatcgacc ggctcctact gtcgcccgat
     1321 gcgggttgct tcggatcaat ccgtccgcaa ggacgaggcc cgcaggagcc gcatgccgtt
     1381 ggcggtcacc agcagcgagg cgccgacgtc ggcaaagacc gcctcccaga gggtggcggc
     1441 accggccacc gccagcacca acacgatcag cttgaggccc agcgccaggc tgacgttctc
     1501 gatgatgacc cggcgcgcgg cgcgcgagag gcggatgacc ccagccaggc gcgacaggtc
     1561 gtcgctcaag agcgccacat cggcggtctc cagcgcggcg tggctgccgg cggcgcccat
     1621 cgcgatcccc agcgaggcgc gtgccagcgc cggggcgtcg ttgactccgt cgccgaccat
     1681 gccgacccgc ccgtagcgcc ccagcaggcg ctcgaccgcc gtcaccttct gctccggcag
     1741 cagctcggcc tcgaccgcgt cgacgccggc cgcccgcgcc accgcctcgg ctaccggaag
     1801 gcggtcgccg gtcagcatca cggtcgtgtc gatgcccgcc tgcttgagcg cggctacggc
     1861 ggcgcgcgcc tccggccgca gctgatccgc caagccaagc agcccaatgg tgcgcccacc
     1921 ggcgttgacc accaccgccg tctgcccgcc ggcctccagc tcggccagcc gctcctccag
     1981 cagctggtcg ccgttgccgg cgaaggcgcg cgtgccgacg cggactaacg cgccgtcgac
     2041 gcgcgcctcg atgccgccgc ccggctgggc gcgcaccgcg ctcgccggga gcggcgtcag
     2101 accgcgcgcc tcggcggcgc gcacgatggc ggcgccgaga ggatgctcgg agtagcgctc
     2161 ggcggcggcc gccagcgcca gcagttcatc ggccgtggcg ccgtcgaccg gcgtcaccgc
     2221 ggtgacggcc agccgcccct cggtcagggt gccggtcttg tcgaaggcca gcgcccgcag
     2281 ttggccggcg gtctcaaggt gcgcgccgcc cttgatcagc acgccgcggc gcgcggcggt
     2341 cgccagcgcg gccacgatcg tcaccggggt ggagatcacc agcgcgcagg ggcaggctac
     2401 gaccagcagc accagcgcgc ggtagagcca ggacgaccag tcgccgccga agaggagcgg
     2461 cggcgctagc gccagcagca ccgccccggc cacgaccgcc ggggtgtagc gccgcgcgaa
     2521 ggcgtcgacc agccgttgcg tcggcgcgcg ccgcgcctgc gcctcctcga ccaggtggat
     2581 gatgcgcgcc agggtcgagt cgctggccgg gcggctggcg cgtacttcga gcacgcccgc
     2641 gccgttgacc gtgccggcga agacctcgtc gcccggtgct ttctcgaccg gtacgctctc
     2701 gccggtgatc ggcgcctgat cgaccgccga gacgccactc gccaccacac cgtcggccgg
     2761 gacgcgctcg ccggggcgca cgatgaccag atcgccgggc gcgacggccg aggcaggcac
     2821 tcgctgctcg ccgtgggccg tgcgcagcac tgcctcgctc ggggccaggc tgagcagcga
     2881 gcggatcgag cggcgggcgc gctcgacggt gtagccctcc agcgactcac cgatggagaa
     2941 gaggaccacc accgtagcgg cctccagcca gtcgccgagc gccgccgcgc cgagcacggc
     3001 gatgatcatc agcacgttga tgtcgagctg gtggcccagg cgcaggctgg cccagccccg
     3061 gcgtgcgacc ggcaccccgg cgatcagggc cgccagggcg tagagggcgg tgccgagcga
     3121 cccagcgccg gccagcgatg ccgccagtcc ggcaaccacc agcgccgctc cgctggcggt
     3181 cagccgggtg gcacggtcgc ccggcagccg ccagccctgc cgcggctgct ccgcctcggc
     3241 ggccggctcg ctgacgcggt agcccgcctc ggccacgcgt cgcgtgatcg cctcgatgtt
     3301 cgtctcaccg ggcacgtact cgaccgccat acgcgcgccg aggaagttga ccgaggcgtc
     3361 gcgtaccccg gccagcccgg agacggcacg ctggagcgtc ttggcgcagt cggcgcaatc
     3421 catcccctcg atcggcagga cgcgatgggc gtagtcctcc cccagccgcc ggccctcctg
     3481 gcgtgcctgc tcgacgagcg tgtcggcggt cgtcaaggtc gggttgaagt gcaccatcgc
     3541 cacgccgtgg gcggggttga gcgccacctg gttgacgccg ggcgaggcct gcaaggtggc
     3601 ctcgaagcgc gccaggcagt gctggcaggt gagcgcctcg gcggggagga ccgaattgat
     3661 atcgagctgc gtggtcgcca tcaggccgtc ttccccctct cctcgttcct ggccaggcga
     3721 tggcgcacgt ggtcgaggcc ctggtcgaag agggccgcca cgtgggcgtc gtcgagcgcg
     3781 tagtagacca gccggccgtc acgccgggcg cgcaccagcc ccagctcgcg cagcagccgc
     3841 aggtggtgcg agacggccga ttgcgacagt ccgcaggtgg cggccaggtc gcagacgcac
     3901 agctcggcgc cggccagcgc ggctaccagc ctcagccggg tggggtcccc gaccgcggcg
     3961 aagagcgccg agagctcggc gatggcggcg gcctccggca accgggcgcg cgagcgctgc
     4021 acggcgtcga ggtggacgat cgggtccgcg caggtttcga gctgcgcggg ctcccggatc
     4081 tgatcgcgct tgcggggacg ggccaccctg cactccaatc acatgagtag ttattcagat
     4141 gttatgaagc atagcgcgtc ggtttgcggg cgtcaagcga tttgaggaga gttgcccatc
     4201 gaggagcatc ggtgccggtt gaggacggat ccctcgactg cgctcaggat gacagggtta
     4261 gggagcggcc tttcggacga ggactgcct
//
LOCUS       DASUBI010000044         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_748529, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000044 DASUBI010000000
VERSION     DASUBI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748529"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /gene="rlmB"
                     /locus_tag="VFU72_00750"
     CDS             <1..678
                     /gene="rlmB"
                     /locus_tag="VFU72_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5422043.1"
                     /translation="TPRCLYLAEGVERQERVAGLLAEAERRRLPVKRLKAPALEQLAP
                     HVNHQGVLLEVGAYPYVQLDQLLAGAGGRPLVVLDHIQDVQNFGTLIRTADATGAAGL
                     IIPDRRGVSVTPAVVNSSAGAVEHVAIARVPNLARALDETKEAGYWAVGLAAEPPAES
                     IFRSDIPEPALLIVGSEGGGIGPALRKHCDLLVQLPMLGKVESLNAAVAGSVALYELL
                     RRRLPAD"
     gene            834..1403
                     /gene="rplI"
                     /locus_tag="VFU72_00755"
     CDS             834..1403
                     /gene="rplI"
                     /locus_tag="VFU72_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5422044.1"
                     /translation="MKVILLRDVAKLGETGSIQTVKDGYARNYLIPKGLAEEATPGKL
                     KNAQQSIAAAERRVAKEEQAKQTLADRIEGTRIHLVARVGEQGRLYGSITAQDIAEKL
                     SQAIGETVERRQVVMEEPIRTVGEHQVAIHLVGRLRPTITVVVTPEAGVEGAFGEVET
                     SPAAEGEASSAQAGPAAEVEDEMRIVPEQ"
     gene            1428..>3125
                     /gene="dnaB"
                     /locus_tag="VFU72_00760"
     CDS             1428..>3125
                     /gene="dnaB"
                     /locus_tag="VFU72_00760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5422045.1"
                     /translation="MIDAVEKLPPSNLEAEQSVLGSLLIDRDAVIRVASFLKPEDFYS
                     GGNGAVYQAVLDLYNRRVPPDFVTVVDELERRERLQDIGGVSYLTGLIETVPTAVHIE
                     YYARIVERTATLRRLIDAGAEIIGIGYDEGVEVEDALDKAERAIFNVSQKRTLRDFIP
                     IGEVLEHYFDKLDTIQQHRGEVVGVPTGYADLDKLTGGLQRSDLIILAARPSVGKTSL
                     QLGMAHSAAVKHGKSVGIFSLEMSAEQLVQRLLAMETGVDSHRLRLGFIDDTEWDHIS
                     RAFGRLAEANIFIDDTPGISVMELRSKARRLLAERGLDLVIVDYLQLAAGRRSENRVQ
                     EISEISRGLKGLARELNVPVLALSQLSRAVESRADHRPMLSDLRESGCLAGETVILDP
                     LTGRPRRIDQMVGDVGAQVWGIDEHFKLGVHPVSRAFCTGIKPLYEVRLASGRRIQAT
                     ANHPFRTIHGWQALEELPPASVVATARHVPEPDVPTAMSEPELVLLAHLIGDGCIAPR
                     QPIHYTSADPANIEAVRGAAEAAFGIKPRVVRQENWWHVYLPSPTRLTRGKPHPITRW
                     "
BASE COUNT          530 a         1063 c         1057 g          475 t
ORIGIN      
        1 accccgcgct gcctctacct ggccgaaggc gtcgagcgcc aggagcgcgt ggccggcctg
       61 ctggccgagg ccgagcgccg ccggctgccg gtcaagcgac tgaaggcgcc cgcgctggag
      121 cagttggcgc cccacgtcaa tcaccagggc gtgctgctgg aggtcggcgc ctacccgtac
      181 gtacagttgg atcaactgct ggctggcgcc ggcggccggc cgctggtcgt gctcgaccac
      241 atccaggacg tgcagaactt cggcacgctg atccgcaccg ccgacgcgac cggcgcggcc
      301 gggttgatca tccccgaccg ccgcggggtc agcgtcacgc cagcggtggt caactcctcg
      361 gccggcgccg tcgagcacgt cgccatcgcg cgcgtgccga acctggcgcg cgcgctggat
      421 gagaccaagg aagccggcta ttgggccgtc ggactggccg ccgagccgcc cgcggaatcg
      481 atcttccgca gcgacatccc tgagccggcg ctcctgatag tcggctcgga gggcggcggc
      541 atcgggccgg ccctacgcaa gcactgcgac ctgctcgtcc agctgccgat gctgggcaag
      601 gtcgagtcgc tcaacgcggc ggtcgccggc tccgtcgccc tgtacgaact gctcaggaga
      661 agactgccgg cggattgaat tcggctctcc ggcacggtat agtaatggcg gagggagagg
      721 tgttcgtaca cacgaacacc gtttttgtcg cacgcggcgg gcagggagcc agcaacgcag
      781 cattcgttgt cgctcgcggc cggcgcggcg ggtatgtgaa gggtaccgct ggtatgaagg
      841 tcattctgct tcgagacgtg gccaagctcg gcgagaccgg ctcgattcag acggtcaagg
      901 acggctacgc acgcaactac ctgatcccca aggggctcgc tgaagaggcc acgccgggca
      961 agctcaagaa cgcgcagcag agcatcgcgg ccgccgagcg ccgtgtcgcc aaagaggagc
     1021 aggccaagca gaccctggcc gaccgcatcg aaggcacgcg catccacctc gtcgcgcgcg
     1081 tcggcgagca gggccgcctg tatggctcga tcacggcgca ggacatcgcc gagaagctct
     1141 cgcaggccat cggcgagacg gtcgagcgcc ggcaggtggt gatggaggag ccgatccgca
     1201 ccgtcggtga gcatcaggtc gcgatccacc tggtcggccg actgcggccg accatcaccg
     1261 tggtcgtgac gcctgaagcc ggtgtcgagg gcgccttcgg cgaggtggag acatccccgg
     1321 cggccgaggg cgaggcgtcg agcgcacagg ccggaccggc cgccgaggtc gaagacgaga
     1381 tgcggatcgt gcccgagcag taggatctat ccggggggac aacgccggtg atcgacgcgg
     1441 tcgagaagct gccaccgagc aatctggagg ccgagcagtc ggttctcggt agcctgttga
     1501 tcgaccgcga cgcggtcatc cgcgtcgcct ccttcctcaa gccggaggac ttctacagcg
     1561 gaggcaacgg cgcggtctat caggccgtgc tcgacctcta caaccgccgg gtgccgcccg
     1621 acttcgtcac cgtcgtcgac gagctggagc gacgtgagcg cctgcaggac atcggcggtg
     1681 tctcctacct gaccggcctc atcgagacgg tcccgaccgc ggtccacatc gagtactacg
     1741 cgcgcatcgt cgagcgcacc gccaccctgc ggcgactgat cgacgccggc gccgagatca
     1801 tcggcatcgg ctacgacgag ggcgtcgagg tcgaagacgc gctcgacaag gccgagcgcg
     1861 ccatcttcaa cgtctcgcag aagcgcaccc tgcgcgactt catccccatc ggcgaggtgc
     1921 tggagcacta tttcgacaag ctcgacacca tccagcagca tcggggcgag gtcgtcggcg
     1981 tgcccaccgg ctacgccgat ctcgacaagc tgaccggcgg cctgcaacgc tcggatctga
     2041 tcatcctggc cgcgcgcccc tcggtcggca agacatccct gcagctcggc atggcgcaca
     2101 gtgccgcggt caagcacggc aagagcgtcg gcatctttag cctggagatg tcggccgagc
     2161 aactggtgca gcggctgctg gcgatggaga ccggggtcga ctcccaccgc ttgcggctcg
     2221 gcttcatcga cgacaccgag tgggaccaca tctcgcgcgc cttcggccgc ctggccgaag
     2281 ccaatatctt catcgacgac acgcccggca tcagcgtgat ggagctgcgc agcaaggcgc
     2341 gacgactact ggccgagcgc ggcctcgacc tggtgatcgt ggactatctg cagctggcgg
     2401 ccggccgccg ctcggagaac cgcgtgcagg agatctccga gatctcgcgc ggcctgaagg
     2461 gactggcccg cgagctgaac gtgccggtgc tggccctctc ccagctctcg cgtgccgtcg
     2521 agagtcgcgc cgaccaccgc ccaatgctct cggatcttcg tgaaagcggc tgcctggccg
     2581 gcgagaccgt catcctcgat ccgctgaccg gccgcccgcg gaggatcgac cagatggtgg
     2641 gcgatgttgg ggcgcaggtc tggggcattg acgagcattt caagctcggt gtgcatccag
     2701 tcagccgagc gttctgcacc ggaatcaaac cgctctatga agtgcgcctt gctagtgggc
     2761 ggcgcatcca agcgaccgcc aaccaccctt tccgcacgat ccacggctgg caggcgcttg
     2821 aggaactacc tcctgcttcg gtcgtggcga cagcccgtca tgtgccggag ccggatgtac
     2881 cgaccgcgat gagtgagcct gagttggtct tgttggctca tctgatcggc gacggctgta
     2941 tagccccgcg ccagccgatt cactacacca gcgccgatcc ggccaacatc gaggcggtgc
     3001 gcggggccgc cgaggcggcg tttggcatca agccccgcgt cgtgcgccag gagaattggt
     3061 ggcacgttta tcttcccagc ccgacgcgtc tcacccgcgg taagccgcac ccgatcacac
     3121 gctgg
//
LOCUS       DASUBI010000045         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_911815, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000045 DASUBI010000000
VERSION     DASUBI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911815"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1453)
                     /locus_tag="VFU72_00765"
     CDS             complement(152..1453)
                     /locus_tag="VFU72_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422046.1"
                     /translation="MSGNVVGRMRVLLLEDDQGLVEHVRGLLRDEDMELNVTTGDIEQ
                     GLRQARNWSPDVILAAQERGHLPDIIEKLDASYSSTPIIALLTPEQAETAHEVLLAGA
                     RAYLPADTGRDELVDTIVSVLERERRRRTALARKLGVEINLGQVVAVHGAKGGVGATT
                     VAANLAVAVQLASRARVALVDANLYSGDVAATLNLMSRGSLADLTPHLKELDQEFLER
                     AAVRHASGLRAFLAPDDFVRAEVISGEQIQRILKVMRQHYDYIIVDTCSLPDQVTTAA
                     MEEADRIVLVVTPELPALKNAARFLQLAGEFGYQGKVTLALNRANSRGALGLNDIQTH
                     LRATVAVSIGSDGKAVMRAVNTGDPVIGQRRSRFNNGIWHLTALITGQQARKLKRLRR
                     PPMPTPDAQPTTPGLDGHSPAPAANDARTRSGLLARLRPGQ"
     gene            complement(1509..>2144)
                     /gene="cpaB"
                     /locus_tag="VFU72_00770"
     CDS             complement(1509..>2144)
                     /gene="cpaB"
                     /locus_tag="VFU72_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU5422047.1"
                     /translation="LTMQPFPADFVPQGAVAQPEQAVGKYATTHITKGQIVLSNQLSP
                     TKRVGQLSLSVPSGKVAIALPMTDLMSTNGAIKAGDHIDIVLTLDLKEIRLDSAQAGA
                     APASSAGGDAKNPVTQTTLQNVEVLSVGQTEDTSSSASSATTSGNNRTQAVIVLLDPQ
                     DALVLDYAKNSGGIVDLALRSPDDTQQVKTDSVTIDYLFQRFNFQRPGPVP"
BASE COUNT          288 a          713 c          786 g          357 t
ORIGIN      
        1 ggcttcgtaa agactagcgc ggtggcttta gccccgcgcg actcgggctg agcggcccca
       61 cgaaacgcga caaacgaccc gccggccccg cgcgacagcc ggcaccgggc catcgggacc
      121 gcggcacacc gtccacccac ctccatcacc cctattgccc cggtcgcagg cgggccagca
      181 ggcccgagcg ggtgcgggcg tcgttggccg cgggggccgg ggaatggcca tcgagtccgg
      241 gcgtcgttgg ctgggcgtcg ggggtgggca tgggtgggcg gcgcaggcgc ttcagtttgc
      301 gcgcctgctg gccagtgatc agcgccgtca gatgccagat gccgttgttg aagcggctac
      361 ggcgctggcc gatcaccggg tcgccggtgt tgaccgcgcg catgaccgcc ttgccgtcgc
      421 tgccgatcga tacggccacg gtggcgcgca ggtgggtctg gatgtcgttc aggccgagcg
      481 cgccgcggct gttggcgcgg ttaagcgcca gggtgacctt gccctggtag ccgaactcgc
      541 cggccagctg caggaagcgc gcggcgttct tcagcgccgg cagctccggg gtgacgacca
      601 gcacgatgcg gtcggcctcc tccatggcgg ccgtggtcac ctggtcgggc agcgagcagg
      661 tgtcgacgat gatgtagtcg tagtgctggc gcatcacctt gaggatgcgc tggatctgct
      721 caccgctgat cacctcggcg cgcacgaagt cgtccggcgc caggaaggcg cgcagcccgc
      781 tggcgtggcg caccgcggcg cgctccagga actcctggtc cagctccttc aggtgcggcg
      841 tcaggtcggc caggctgccg cgcgacatca ggttgagcgt ggcggccacg tcgccggagt
      901 agagattggc gtcgaccagc gccacgcggg cgcggctggc cagctgcacc gccaccgcca
      961 ggttggccgc cacggtggtg gcgccgacgc cccccttggc gccgtgcacg gccacgacct
     1021 gccccaggtt gatctcgacg cccagctttc tggctagcgc ggtgcggcgg cggcgctcgc
     1081 gctccagcac gctgacgatg gtatccacca gctcgtcgcg ccccgtgtcg gcggggaggt
     1141 aggcgcgggc gccggccagc agcacctcat gcgcggtctc agcctgctcc ggcgtcaaga
     1201 gcgcgatgat cggcgtgctg ctgtaggagg cgtccagctt ctcgatgatg tccggcaggt
     1261 ggccgcgctc ctgagccgcc aggatgacgt cgggcgacca gttgcgcgcc tggcgcagac
     1321 cctgctcgat atcgccggta gtgacgttca gctccatgtc ctcgtcgcgc agcaggccgc
     1381 gcacgtgctc caccagcccc tggtcatcct ccagcaggag gacgcgcatt ctgcccacga
     1441 cattcccgct cattcgtgac gaatcctttc cgctgctatg cgcggccggc gatgacccgg
     1501 ggcgacccct acggcaccgg ccccgggcgc tggaagttga agcgctggaa gagatagtcg
     1561 atggtgaccg agtcggtctt gacctgctgg gtgtcgtcgg gcgagcgcag cgccaggtcg
     1621 acgatgccgc cggagttctt ggcgtagtcg agcaccagcg cgtcctgcgg gtcgagcagc
     1681 acgatcaccg cctgcgtgcg attgttgccg gaggtggtgg cgctgctggc gctactggag
     1741 gtatcctcgg tctgaccgac ggagaggacc tcgacgttct gcagcgtggt ctgcgtcacc
     1801 gggttcttgg catcgccgcc ggcgctgctg gccggcgcgg cgccggcctg cgcgctgtcg
     1861 agccggatct ccttcaggtc gagcgtcaac acgatatcga tgtggtcgcc ggccttgatc
     1921 gcgccgttgg tgctcatcag gtcggtcatc ggcagcgcta tggccacctt gcctgaggga
     1981 actgagaggg agagctggcc gacgcgcttg gtcggcgaga gctggttgga gagaacgatc
     2041 tgccccttgg tgatgtgggt ggtggcgtac ttgccgacgg cctgctcggg ctgggccacc
     2101 gcgccctggg gcacgaagtc ggccgggaag ggctgcatgg tcag
//
LOCUS       DASUBI010000046         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1115399, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000046 DASUBI010000000
VERSION     DASUBI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115399"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFU72_00775"
     CDS             <1..317
                     /locus_tag="VFU72_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422048.1"
                     /translation="VLPVGEYFAKSSPDVLLAAAAEIEGLVIVTFDRDFRRLVRQLPS
                     GVRTQAAHHAGRISVTCTETELLGRLRTLIDVIEFHYAFSQRQGHRLIMQISATSYTF
                     VE"
     gene            complement(328..762)
                     /locus_tag="VFU72_00780"
     CDS             complement(328..762)
                     /locus_tag="VFU72_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5422049.1"
                     /translation="MTYPDPQVSAAILERFIPLKLDLFRDPREVLRPLDVIWTPTILF
                     ADRRGTPHYRSLNFLPPELFLALLDIGEANIDLRWSRNEHAIALLRGAYERDPDGRLA
                     PEVLYWWGIAVYLQTHSDPAMYEIWDILRQRFPESIWSARVP"
     gene            complement(879..1385)
                     /locus_tag="VFU72_00785"
     CDS             complement(879..1385)
                     /locus_tag="VFU72_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422050.1"
                     /translation="MADLEELKQAIDAGHERVLAALAGIPETELETAPAVGAWSARDV
                     VGHLADWEGETLAAAAHILGGPKPRFHPIKDGQQYNSLRAALWGTEPWQIALEDFARA
                     RGRAEAFLADLAPEQLQAIGPFPWGEVGRLGKLLEDLVSHLDEHAAQLEEWRLRRAGI
                     HEPRRGRW"
     gene            complement(1385..>3089)
                     /locus_tag="VFU72_00790"
     CDS             complement(1385..>3089)
                     /locus_tag="VFU72_00790"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5422051.1"
                     /translation="ARAVNPHFRITPENLAAVAGICRRLDGLPLAIELASARAAVLAP
                     EDLLERLERRLPVLTGGTRDLPERHQTLRQAIAWSYDLLNDPEQRLFRRLAVFGGGFS
                     LAAAEAIGGADLAGGVLDLLTSLLDKHLVQAAAEPDGMRFSLLETVREFGLECLAASG
                     ELAGVAARHGEHFVQLAEAGYGRQVGSAQASWFRRLERELGNLRAAARWIVEQQDAGR
                     AARLGLSLWRFWDRGYIVEGRAWLESFLGLPGLAAPGPARCRLLFAAGRLAYRQADYS
                     PAAALLTECLAIARAERDDDFTASALTQLAHLAYAQGDLEIAERRYAESLDIRRRAGD
                     DARTIAVSLYGLARVHRARADYAVARALLVESLEQFQAAEDDVQIAGVLVALGLVALL
                     EGDDEEAGGAFRESLARALKVGDRPGEAGALIGLAYAALGRGQPRRARTLLGESLALA
                     RATDGRQLVVQCLEGMAAVLAADGEGRRAWRLAAAVAAYRARAAVPQDPSERVLLARY
                     LDPVAATLQPGERAALWATGSALTPEEALAGIEWPGVVVPLESIRANLVQYEREREVR
                     A"
BASE COUNT          458 a         1144 c         1078 g          409 t
ORIGIN      
        1 tcgtgctgcc cgtcggcgag tacttcgcca agtcctcgcc ggacgtgctc ctggctgcgg
       61 cggcggagat tgaaggcctc gtcatcgtca ccttcgaccg cgacttccgg cgcctggtgc
      121 ggcagcttcc ctccggcgtg cgtacgcagg cggcgcacca tgccggacgg atcagcgtga
      181 cctgcaccga gacggagttg ctcggccgcc tgcgcacgct catcgacgtc atcgagttcc
      241 attatgcgtt cagtcagcgg cagggacatc gcctgatcat gcagatcagc gcgaccagct
      301 acaccttcgt cgagtgagtt ccgccactca cggcacccgc gcgctccaga tcgactctgg
      361 gaagcgctgg cgcaggatgt cccagatctc gtacatggcg gggtcggagt gggtctgcag
      421 gtagacggcg atgccccacc agtagaggac ctcgggcgcc aggcggccgt cggggtcgcg
      481 ctcgtaggcg ccacgcagga gcgcgatggc gtgctcgttg cgcgaccagc gcaggtcgat
      541 gttggcctcg ccgatatcca gcagcgccag gaagagctcg ggcggcagga aattcaggct
      601 gcggtagtgc ggcgtgccgc gccggtcggc gaagaggatc gtcggcgtcc agatcacgtc
      661 gagcgggcgg agcacctcgc gcggatcgcg gaagagatcg agcttgagcg ggatgaagcg
      721 ttcgaggatc gcggcgctga cctggggatc gggatacgtc acggtatcca gcttgaggca
      781 gccacgtcag gggaccttca tcacgtcgat caggatcggc ttgcgctcgg cgcgcgagcg
      841 ctccatggcg cggttgaagt cgcgctccca ctcgatggct accatcggcc cctcctcggc
      901 tcgtggatgc cggcgcggcg caggcgccac tcctccagtt gggcggcgtg ctcgtcgagg
      961 tggctcacca gatcctccag cagcttgccg agccggccga cctcgcccca ggggaagggg
     1021 ccgatcgcct gcaactgctc cggcgcgagg tccgccagga acgcctcggc gcgcccgcgg
     1081 gcacgggcaa agtcctccag cgcgatctgc cagggctcgg tgccccagag cgcggcgcgc
     1141 agggagttgt attgctgccc gtccttgatc gggtggaagc gcggcttggg gccgccgagg
     1201 atatgcgcgg cggcagccag cgtctcgccc tcccagtcgg ccaggtggcc gaccacgtcg
     1261 cgcgccgacc aggcgccgac ggccggcgcc gtctccagct cggtctcggg gatgcccgcc
     1321 agcgccgcca gcacccgctc gtgccccgcg tcgatcgcct gcttgagctc ctcgagatcg
     1381 gccatcacgc cctcacctcc cgctctcgct catattgaac gagattcgct cggatggatt
     1441 caagcgggac aacgaccccg ggccactcga tgccggccag cgcctcctcc ggcgtcagcg
     1501 cgctcccggt cgcccagagc gccgcgcgct cgcccggttg cagcgtggcc gcgacgggat
     1561 cgagataccg tgctagcaag acccgctcgc tgggatcctg cggcaccgca gcacgcgcgc
     1621 ggtaggcggc gaccgccgcg gccagccgcc aggcgcgtcg cccctcaccg tcggccgcca
     1681 gcaccgccgc catgccctcc agacactgga ccacgagctg ccgcccatcg gtcgcacgcg
     1741 ccagcgccag gctctcgccg agcagcgtcc gcgcccgccg cggctgcccg cgcccgagcg
     1801 ccgcgtacgc cagcccgatc agcgcgccgg cctcacccgg ccgatcccca accttgagcg
     1861 cgcgtgccag gctctcgcga aacgcgccgc cggcctcctc gtcgtcgcct tccagcagcg
     1921 caaccaggcc gagcgccacc agcacacccg cgatctgcac gtcatcctcc gccgcctgga
     1981 actgctctag gctctcgacc aagagcgcgc gcgcgacggc gtagtcggca cgggcgcgat
     2041 gcacgcgcgc caggccgtag agcgagacgg cgatggtgcg tgcgtcgtcg ccggcacggc
     2101 gacggatgtc caggctctcg gcgtagcggc gctcggcgat ctccaggtcg ccctgggcgt
     2161 aggccaggtg ggcgagctgg gtcaacgcgc tggcggtgaa gtcgtcgtcc cgctcggcgc
     2221 gggcgatggc caggcactcc gtcagcagcg cggcggccgg gctgtagtcg gcctggcggt
     2281 aggcgagccg cccggcggcg aagaggaggc ggcagcgcgc cgggccaggc gcggccagac
     2341 cggggagccc gaggaaggac tccagccagg cgcgcccctc cacgatatag ccgcgatccc
     2401 agaagcgcca gaggctcagg ccgagccggg ccgcgcgccc ggcatcctgc tgctcgacga
     2461 tccagcgcgc ggccgcccgc aggttgccca gctcgcgctc caggcggcgg aaccagctcg
     2521 cctgcgcgct ccccacttgc cggccatagc ccgcctcggc caactggacg aaatgctcgc
     2581 cgtggcgggc ggcgacgccc gccagttcac cggaagccgc cagacactcc agcccgaact
     2641 cccggaccgt ctccagcaac gaaaagcgca tgccgtccgg ctcggcggcg gcctgcacca
     2701 gatgcttatc gagcagcgag gtcaagaggt ccagcacgcc gccggccagg tccgcaccac
     2761 cgatcgcctc ggcggccgcg agggagaagc cgccgccgaa gacggctagc cgccgaaaga
     2821 ggcgctgctc cgggtcgttg agcaggtcgt agctccaggc gatcgcctgc cgtagcgtct
     2881 gatgccgctc cggcagatcg cgcgtcccgc cggtgagcac cggcaaacgg cgctccagcc
     2941 gctccagcag atcctcgggc gcgagcaccg cggcgcgggc cgaggccagc tcgatggcga
     3001 gtggcaagcc gtcgagccgg cggcagatgc cggcgaccgc cgccagattc tcgggggtga
     3061 tacggaaatg cgggttgacc gcccgcgcc
//
LOCUS       DASUBI010000047         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_25434, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000047 DASUBI010000000
VERSION     DASUBI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25434"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /locus_tag="VFU72_00795"
     CDS             complement(<1..2469)
                     /locus_tag="VFU72_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5422052.1"
                     /translation="MDVFRLREALIGEYAAYIQSFVRIRDTRIARQVQEALDEGLLWP
                     EPLIGLNPTFAPGGWVDDLVAEGVLHPECRAIFRLKPDPHDRTHDRPLRLHQHQADAI
                     RTARGGHNYVLTTGTGSGKSLAYIIPIVDSVLRHGTGRGIQAIIVYPMNALANSQAGE
                     LEKFLRHGYPAGQGPVTFARYTGQESDEERQAIIASPPDILLTNYVMLELLLTRPQEA
                     PLIRAAQGLRFLVLDELHTYRGRQGADVALLVRRTRDLLAADALQCVGTSATLAGAGS
                     FAAQRAEVAAVATRLFGAPVLPQYVIGETLRRVTPEPDLDDPAYRAALRARLEQRDPP
                     ASDYAALVADPLAAWLESTLGVTAEPDTGRLVRSTPRGIGGPDGAAAALSALTGSAPD
                     CCRQALEELLLAGYACAQPETGFPVFAFRLHQFVSGGDTVYATLEPEDQRHLTVYGQK
                     SAPGGRLLVPLVFCRECGQEYYAVRRVTDPQTGLAAFLPRPPLDREEEGEPGYLFAST
                     DNPWPAGDEARNARLPDDWLEPGPGGVLRPRASRRPFLPQPVTVDAEGAQAAAGLAAH
                     FLPAPFQFCLHCGVAYNPNQRSDYGKVSALSAGGRSSATTIMSLFAIRFLREALDLRS
                     EARKLLSFTDNRQDASLQAGHFNDFVDTGLLRAGLYRAVQAAGAEGLGHEELTQAVFA
                     ALALPLDAYAADPAVRYQALADTKRALRDVLGYRLYRDLKRGWRVTAPNLEQCGLLEI
                     AYLSLDDLCATQEDWQETHAALAGATPATRQQLARTLLDYLRRELAVQVQYLDREYQE
                     RLQQQSGQLLVPPWALDEYERLEVA"
     gene            3164..>3936
                     /locus_tag="VFU72_00800"
     CDS             3164..>3936
                     /locus_tag="VFU72_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5422053.1"
                     /translation="MRAGIYFRVSSEEQVDGYSLDAQQRALLDYCAAREWTVVEEYAD
                     EGKSARTDKLERRPAFKRMMVDAQAGALDVVVVHKIDRFARNIRVTFECLELLARYHV
                     AFVAVAQPDLDYTRAEGRLFMGMMATLAQYYSDNLSQETKKGKAERKTQGLYNGHIPF
                     GMIKGPDGVPVANPDTIAGLRLAFQQAAKGQSDRQIAQALNLAGYRTTGLRTGKPELF
                     SKDTVRTMLQNHFYLGELPGERPANAAPVQHQPVIGSVL"
BASE COUNT          654 a         1282 c         1383 g          617 t
ORIGIN      
        1 tgccacttcc agccgctcgt attcatcgag cgcccacggc gggacgagga gctggccact
       61 ctgctgctgc aagcgctcct ggtactcacg gtcgaggtat tgcacctgca ccgccagttc
      121 gcggcgcagg tagtcgagca gcgtgcgggc gagctgctgg cgggtggccg gcgtcgcgcc
      181 ggcgagcgcg gcgtgggtct cctgccagtc ctcctgggtc gcgcagaggt cgtcgagcga
      241 gagataggcg atctccagca ggccgcactg ttccaggttc ggcgcggtga cgcgccagcc
      301 ccgcttgagg tcgcggtaga ggcggtagcc cagcacgtcg cgcagcgccc gcttggtgtc
      361 ggccagcgcc tggtagcgca cggcggggtc ggcggcgtag gcgtcgagcg gcagcgcgag
      421 cgcggcgaag actgcctggg tcagttcctc gtggcccagc ccctccgcac ccgccgcctg
      481 caccgcccgg tacagcccgg cgcgcaagag gccggtgtcc acgaaatcgt tgaaatggcc
      541 ggcctggagc gaggcgtcct ggcggttgtc ggtgaaactg agcagtttgc gcgcctcgga
      601 ccgcaggtcg agcgcttcgc gcaggaagcg gatggcgaag agactcataa tcgtcgtcgc
      661 gctgctacgc ccgcccgccg acagggcgga gactttgccg taatccgagc gctgattggg
      721 gttgtaggcg acgccgcagt ggaggcagaa ctggaagggc gcggggagga aatgcgccgc
      781 caggccagcc gcggcctgcg cgccttctgc atccacggtg accggctgcg gcaggaaggg
      841 ccggcggctg gcgcgcgggc ggaggacgcc gccggggccg ggttccagcc agtcgtccgg
      901 caggcgcgcg ttgcgcgcct cgtcgccggc gggccaggga ttgtccgtgc tggcgaagag
      961 gtagcccggt tcgccttcct cctcgcggtc gagcggtggg cgcggcagga aggcggccag
     1021 cccggtctgc gggtcggtga cccggcgcac agcgtaatac tcctggccgc actcgcggca
     1081 gaagacgagc ggcaccagca gtcggccgcc cggcgcgctc ttctggccgt agaccgtcag
     1141 gtggcgctgg tcctccggct ccagcgtcgc gtagactgtg tcgccgccgc tgacgaactg
     1201 gtggagccgg aaggcgaaga ccgggaagcc ggtctccggc tgggcgcagg cgtagccggc
     1261 cagcaggagt tcctctagtg cctggcgaca gcagtccggc gcgctgccgg tcagggcgct
     1321 cagggctgcc gccgcgccgt ccggaccgcc gatgccgcgt ggcgtgctgc ggacgaggcg
     1381 gccggtgtcc ggctccgccg tgaccccgag ggtgctttcg agccaggccg caagtgggtc
     1441 ggcgacgagc gcggcatagt cgctggccgg tgggtcgcgc tgttccagcc gggcacgcag
     1501 cgcggcgcga taggcggggt cgtcgagatc cggctccggc gtgacgcgcc gcagggtctc
     1561 cccgatgacg tattgcggca gcaccggcgc gccgaagagg cgggtggcga cggcggcgac
     1621 ctcggcgcgc tgcgccgcga aactgcccgc accggccagc gtcgctgagg tgccgacaca
     1681 ctggagggcg tcggcggcca gcaggtcgcg ggtgcgccgg acgagcagcg cgacgtcggc
     1741 gccctgccgg ccgcgatagg tatggagttc gtccagcacc aggaagcgca agccctgcgc
     1801 cgccctgatg agcggcgctt cctgtgggcg cgtcagcagc agttcgagca tcacatagtt
     1861 ggtgagcagg atatcgggcg gactcgcgat gatcgcctgg cgttcctcgt cgctctcctg
     1921 gccggtgtaa cgcgcaaaag tcactggccc ctggccggca ggatagccgt ggcgcagaaa
     1981 cttctccagt tcgccggcct ggctgttcgc cagcgcgttc atcgggtaga cgatgatcgc
     2041 ctgaatgccc cggcccgtgc cgtgccgcag cacgctgtcc acgatgggga tgatgtacgc
     2101 caggctcttg cccgagcctg tcccggtcgt caggacgtaa ttgtgcccac cgcgcgccgt
     2161 gcggatggcg tcggcctgat gctgatgaag gcggagcggc cggtcgtgcg tgcggtcgtg
     2221 cgggtcgggc ttgaggcgga agatggcgcg gcattcggga tggagcacgc cctcggcgac
     2281 gagatcgtcc acccagccgc ctggcgcgaa ggtcggattg aggccgatga gcggctccgg
     2341 ccagagcaag ccctcatcca gcgcctcctg gacctgccgc gcgatccgcg tgtcgcgaat
     2401 gcgcacgaaa ctttggatat acgcggcata ctcgccaatc agcgcttcgc gtaaccgaaa
     2461 gacgtccacc cgctccccct gctccctggc actggtcatg ctaacgcgcg gcgcgccccc
     2521 cgcactgtga aggcgatcac cggcagcctg ttcgtccgct atcgcaacca tacgcagtgt
     2581 gagcgcaggg cgcaagcacc cccggaccaa agtactagcg cttctcatgc caagcagcgc
     2641 tacgtcagac atgagtcggt gaccgatgtt gctgccccag tctaggcggc gcctaggacc
     2701 actgcggttc ccgtgcgcct ttgggtgacc tacgttggcc tgaaacaggg ccgccagagt
     2761 ggcggtagcc gcacgacact cggcttgcga aatgcggacg aatacatttt ctaaatgatg
     2821 atatactgtt atactagtat atagaatagt atgaggagaa gcgacgggag ggcgctatac
     2881 cgacactacg cgaggctcga ttagcacgct actggagtat tcgcgatctc gccgtgcgtg
     2941 ccgacattac tccgcgcacc atcgtgcaga tcgagcatgg ccggctcacg ccgcgcctcg
     3001 tcacgatgcg gcgactcgcg tcggcgcttg cgactgagcc gggggccatc gccgagtttg
     3061 cggcggccat ccagcaagct tcgtcttgcc gctaggacca acgcattcgc atcgtgaacc
     3121 aaccggcgcc aatgtcgccg gcatcgagat gaaggagcgg atcatgcggg cagggatcta
     3181 cttccgggtc agcagcgaag agcaggtcga cggctactcc cttgatgccc agcagcgggc
     3241 attgcttgac tactgtgcgg cgagggagtg gacagtcgtc gaggaatatg ccgacgaggg
     3301 caagtcggcg cggaccgaca agcttgagcg ccggccagcc ttcaagcgca tgatggtaga
     3361 cgcccaggct ggcgcactcg acgtggtggt ggtccacaag atcgaccggt tcgctcgcaa
     3421 catccgcgtg acattcgagt gtctcgaact tctggctcgc taccacgtcg cattcgtggc
     3481 ggtcgcgcag cccgacctcg actatacgcg cgcggagggt cgactcttca tgggcatgat
     3541 ggcgacactg gcgcagtact acagcgataa cctgagccag gagaccaaga aaggcaaggc
     3601 cgagcggaaa acgcaggggc tctacaatgg gcatattccg ttcgggatga ttaaaggccc
     3661 ggacggcgtg ccggtcgcca accctgacac catcgcagga ctccgcctgg cgtttcaaca
     3721 ggcagccaag ggtcagagtg accgacagat cgcccaggcg ctcaatctgg cgggctaccg
     3781 aacgaccggc ctccgaaccg gcaagcccga actcttcagc aaagacaccg tccgcaccat
     3841 gctccagaat cacttctatc tcggcgaact gcccggggag cggccggcga acgcggcgcc
     3901 ggtgcagcac caaccagtca tcggctccgt gctctt
//
LOCUS       DASUBI010000048         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_817492, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000048 DASUBI010000000
VERSION     DASUBI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817492"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFU72_00805"
     CDS             <1..1344
                     /locus_tag="VFU72_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5422054.1"
                     /translation="AQDLAERAALAIDNARLYQEARQHAARAIARAEASRAVVAVQFD
                     QRSIIQNITRIVSEQVGDGVVVRLVSGDGQWLDVVAVHHPDPEKHRALGGMVSAARLR
                     IDEGFNAQVLASGQPLHIPAANQDEFHQYLRPEYAGFHQQFGYHTLLIVPLRLRERII
                     GSIALFRDGESGPYSAAERTFVQDLADVAALAIDNARLYQQAQDAVEVRERFLSMASH
                     ELKTPLTTIKALGQLLERLSGQPELDRSRQARLMRQLLQSTERFEELVNDLLDVSRIQ
                     QQRLALHPEALDLRELAGEVLDRFGESSERTDEHRLVLEAPEPVLGEWDASRLDQVLT
                     NLISNALKYSPGGGEVRVTVRQVGERAEVTVADQGIGITAEELRILFQPFSRGERVRA
                     QVEGTGLGLYITHEIVEQHGGTVTVASQPDVGSRFSVRLPLRSAPGEPNGQEREP"
     gene            complement(1363..1575)
                     /locus_tag="VFU72_00810"
     CDS             complement(1363..1575)
                     /locus_tag="VFU72_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422055.1"
                     /translation="MTSEQTSNPQVHAEHIKGMLNDTIQHIRADEQRVDDLQAKALFE
                     TTAEVLGGLVKAYDDYERREPAWRKG"
     gene            complement(1733..2623)
                     /locus_tag="VFU72_00815"
     CDS             complement(1733..2623)
                     /locus_tag="VFU72_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5422056.1"
                     /translation="MFQAVIVPLDGSELAEAALPYARVLATGLHARLTLVSVLGDSAS
                     AQDQQRLRAYLDAQAAALGPGAAVGLRLGDPVKQIVAAGQEQQGAVVVMASQSEHGLS
                     GLFHSSVAAQVVHDSSVPVLVMRAAPAEAVNFARILVPLDGSAFAERALPVAKGLAQA
                     FGSELQLVRVAPVTQLYALSSGSMVDPGAPAVMAEMTNQLIGAARDFITEKTEQVRGE
                     GYQVQGEALLGDPGNQLVTFAKDRKSDLIVMTTHGQGGLMRFLFGSVAKHMLDNAATP
                     LLLVHPTGKVEAAVQGAGNG"
     gene            2787..>3568
                     /locus_tag="VFU72_00820"
     CDS             2787..>3568
                     /locus_tag="VFU72_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU5422057.1"
                     /translation="MVEDARVPADALEEFTTNVLLALDVLPEDARLTAAILIASDVRG
                     IDSHGVPRLRMYVNRIRDGLINLRPNLRVISEAPAAIAFDADNGLGHPASYRVMQRCI
                     EKAQEAGMCMATVRHSNHFGIAGYYATMALEVEGMCGLAMTNATPLLVPTFAREPYLS
                     TAPIAIAIPAGDQWPMVLDMATTTVSWGKVEIARRAEKPIPSGWAYDRAGNITTNPNE
                     AVALTPLGSSRDLGNQKGYGLALFVDVLCGQLAGANWSRYISG"
BASE COUNT          523 a         1264 c         1234 g          547 t
ORIGIN      
        1 gcccaggatc tggccgagcg cgccgcgctg gcgatcgaca acgcacggct ctaccaggaa
       61 gcgcggcagc acgcggcgcg ggccatcgcc cgtgccgaag cctcgcgcgc cgtcgtcgcc
      121 gtgcagttcg accagcgttc gatcatccag aacatcacgc ggatcgtgtc cgagcaggtg
      181 ggcgacggcg tcgtggtgcg gctcgtctcc ggcgacggtc agtggctgga tgtcgtcgcc
      241 gtacaccacc ccgacccgga gaagcaccgg gccctcggcg gcatggtgtc cgccgcgcgg
      301 ctgcgcatcg atgaggggtt caacgcgcag gtcctggcga gcgggcagcc gctgcacatc
      361 ccggcggcca accaggacga gtttcaccag tatctgcgcc cggagtacgc cggctttcac
      421 cagcagttcg gctatcacac gctgctgatc gtgccgctgc ggctgcgcga gcgcattatc
      481 ggctcgatcg ccctctttcg tgacggcgag agcggcccct acagcgcggc cgagcggacc
      541 ttcgtgcagg acctggcgga cgtggccgcg ctggcgatcg acaacgcccg cctctaccag
      601 caggcccaag atgccgtgga ggtgcgcgag cgtttcctct cgatggcctc gcacgagctg
      661 aagacgccgt tgaccaccat caaggcgctg gggcaactgc tggagcggct cagcggccag
      721 ccggaacttg accgctcgcg ccaggcgcgg ctgatgcgcc agttgctgca gtccaccgag
      781 cgcttcgaag aactggtcaa cgatctgctc gatgtctccc gcatccagca gcagcggctg
      841 gcgctgcatc cggaagcgct cgacctgcgt gagctggcgg gcgaggttct ggatcgcttc
      901 ggcgagagct ccgagcgcac ggacgagcat cgactggtgc tggaggcgcc ggagccggtg
      961 ctgggggagt gggacgcctc gcgcctggac caggtgctga ccaatctgat ctcgaacgcg
     1021 ctgaagtact cgccgggggg cggcgaggtg cgagtgacgg tgcgccaggt gggcgagcgg
     1081 gccgaggtga cggtggccga ccagggcatc ggtatcaccg ccgaggagct ccgtatcctc
     1141 tttcagccct tcagccgcgg cgagcgcgtg cgggcgcagg tcgagggtac cgggcttggc
     1201 ctctacatca cgcacgagat cgtcgagcag cacgggggca cggtaacggt ggcgagccag
     1261 ccggatgtcg gcagtcgctt tagcgtgcgc ttgccgctgc gctccgcgcc gggcgaaccc
     1321 aatggccagg aacgggagcc gtaacgcggg ggcgcgctgt cctcagccct tgcgccaggc
     1381 cggctcgcgg cgctcgtagt cgtcgtaggc cttgaccagc ccgccgagca cctcggctgt
     1441 cgtctcgaag agcgccttgg cttgcaggtc gtcgacgcgc tgctcgtcgg cacggatgtg
     1501 ctggatggtg tcgttcagca tccccttgat gtgctcggcg tgcacctgcg ggttggatgt
     1561 ctgctcagat gtcatcgtgg ttcgctcctc tgcggtggtc ccgatcgtca cgggtggtcc
     1621 gcagggggtg tgccgggagg gcgggcgccc ttcgatggag cgtggtgccg cgtggcgcac
     1681 ggatcctgag cttgtcgaag gatccgtcct ggacggcaca atgcctgcct cgttagccgt
     1741 tacccgcgcc ctgcacggct gcctccacct tgccggtcgg gtggaccagc agcagcgggg
     1801 tagccgcgtt gtcgagcatg tgcttggcca cgctgccgaa gaggaagcgc atcagcccgc
     1861 cctggccgtg ggtggtcatc acgatcagat cgctcttgcg gtccttggcg aaggtgacga
     1921 gctgattgcc cgggtcaccg agcagtgcct cgccctgcac ctgatagccc tcgccgcgca
     1981 cctgctcggt cttctcggtg atgaagtcgc gcgccgcgcc gatcagctgg ttggtcatct
     2041 cggccatgac ggcgggcgcg cccggatcga ccatgctgcc gctggagagc gcgtagagct
     2101 gggtcaccgg cgccacgcgc acgagctgca gctcggagcc gaaggcctgc gccagcccct
     2161 tggccaccgg caacgcccgc tcggcgaagg ccgagccgtc gagcggcacc aggatgcggg
     2221 cgaagttgac tgcttccgcc ggcgcggcgc gcatcaccag caccggcacg ctgctgtcgt
     2281 gaaccacctg ggccgccacg ctgctgtgga agagcccgct cagcccgtgc tcgctctggc
     2341 tggccatcac caccacggcg ccctgctgct cctggccagc tgccacgatc tgcttcaccg
     2401 ggtcgcccag ccgcaggcca accgcggcgc ccggccccag cgcggcggcc tgcgcgtcca
     2461 ggtaggcgcg cagccgctgc tggtcctggg cgctcgcgct gtcacccagc acgctgacca
     2521 gcgtcaggcg ggcgtgcaga cccgtggcca acacccgggc gtaggggagc gccgcctccg
     2581 ccagctccga gccatccagc ggcacgatca cagcctggaa catcagcccc tccaacgcct
     2641 cgatgcctcc accttcactg ccgcccgggc gcggcgcagg gtcagtctag cccagccgag
     2701 ccgggcgcgg tatcccggcc ccggcggagc tgcgctatga tgcgggccgg atgccggcga
     2761 tggagtttcg cgagagggag cctgtgatgg tcgaagatgc gcgcgtcccg gcggatgcgc
     2821 tggaggagtt tacgaccaac gtcttgctgg cgctcgacgt gctgccggag gacgcgcggc
     2881 tgaccgccgc catcctgatc gcctccgacg tgcgcggcat cgactcgcac ggcgtgccgc
     2941 ggctgcgcat gtacgtcaat cgcatccgcg acggactgat caacctgcgg cccaacctgc
     3001 gggtcatcag cgaggcgccc gccgccatcg ccttcgacgc ggacaacggc ctcggccacc
     3061 ccgcctccta tcgcgtgatg cagcgctgca tcgagaaggc acaggaggcc ggcatgtgca
     3121 tggccaccgt gcgccacagc aaccacttcg gcatcgccgg ctactacgcc accatggcgc
     3181 tggaggtcga ggggatgtgc ggcctggcga tgaccaacgc caccccgctg ctggtgccca
     3241 ccttcgcccg cgagccctac ctcagcaccg ccccgatcgc gatcgccatc ccggccggcg
     3301 accagtggcc gatggtgctc gacatggcga ccaccaccgt ctcctggggc aaggtggaga
     3361 tcgcgcgccg cgccgagaag ccgatcccgt ccggctgggc ctacgaccgc gccggcaaca
     3421 tcaccaccaa ccccaacgag gcggtcgcgc tgacgccgct cggctcctca cgcgacctgg
     3481 gcaatcagaa gggctacggc ctggcgctct tcgtggacgt cctctgcggc cagctcgccg
     3541 gggccaactg gagccgctac atctccgg
//
LOCUS       DASUBI010000049         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_979549, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000049 DASUBI010000000
VERSION     DASUBI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979549"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFU72_00825"
     CDS             complement(<1..844)
                     /locus_tag="VFU72_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5422058.1"
                     /translation="MGTLHAWRTNPARLRLALLVASSLLIALALAAGRVLGQPGLDAG
                     LMVAAALAAGVDIASRAVRGPRQRQITIELLVVVAAVGALLIGEYWEAAAVTLLFVLG
                     AQLESWTLGRTRQALSQLLRLVPTVALVVRDGEPTAVAPEDVRVGETVLVRPGSRIPV
                     DGVVTGGHAAVDESMITGESLPVEKTAGDRVYAGAVSANGTLWLRAEGIGFDTTLARI
                     IQRVEEAQEEQAPAQRFIERFARWYTPAIIGASLVVFVLTRDVARALTLLVIGCPGAL
                     VISTP"
     gene            complement(844..1059)
                     /locus_tag="VFU72_00830"
     CDS             complement(844..1059)
                     /locus_tag="VFU72_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5422059.1"
                     /translation="MKTVLRGADLNCPSCAVKLERALTALPGVERARVHFATGRIEVE
                     RDDARVAVEDLLKVVRQLGYNVKVAGF"
     gene            complement(1174..1914)
                     /locus_tag="VFU72_00835"
     CDS             complement(1174..1914)
                     /locus_tag="VFU72_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5422060.1"
                     /translation="MTRIRTKPIESRSCTLELRLHLLARLPFFAELTADDIARISQDF
                     HERSFSEGQTIYTGGEPARWLYVVAAGQVKLLRPMPSGQNVLLDILGQGDFFGSLSTL
                     GDRVYPDSAEALTTCCVLAVDAAAFHQILQRYPLAALTTLGIVSERLRTAHDLIEGLS
                     GHDVEQRIAMTLLRLANRAGERREGMLVIQLPLSRQDLADMVGTTRETASRVISQLDR
                     DGVIHSGRGWIGLIDLDQLAAIAEGDAG"
BASE COUNT          332 a          735 c          674 g          283 t
ORIGIN      
        1 ccggcgtcga gatgaccagc gcccccgggc aaccgatcac cagcaacgtc agcgcgcgcg
       61 cgacatcacg cgtcaagacg aagacgacca ggctagcccc gatgatcgcg ggggtgtacc
      121 aacgggcgaa gcgctcgatg aagcgctgcg ccggcgcctg ctcctcctga gcttcctcga
      181 ctcgctggat gatgcgcgcc agcgtggtat cgaacccgat accctccgcg cgcagccaga
      241 gcgtcccgtt cgcgctcacg gcgcccgcgt agacccgatc gccggccgtc ttctcgaccg
      301 gcagcgactc gccggtgatc atgctctcgt ccacggccgc gtgcccgccg gtcacgactc
      361 catcgacggg gatccggctc cccgggcgca ccagcaccgt ctcgccgaca cgcacgtcct
      421 ccggcgcgac cgccgtcggc tcgccgtcgc gcaccaccag cgcgaccgtc ggcaccagcc
      481 gcagcagttg cgacagcgcc tggcgcgtgc gcccgagcgt ccagctctcg agctgcgcgc
      541 ccagcacgaa gagcagcgtg accgccgccg cctcccagta ctcgccgatc agcagcgcgc
      601 cgaccgcggc caccaccacc agcagctcga tcgtgatctg gcgttggcgc gggccgcgca
      661 ccgcacgact cgcgatatcc acgccggccg cgagcgccgc ggcgaccatc agccccgcat
      721 ccaggccagg ctggccgagc acgcggccgg cagccagcgc cagcgcgatc agcaggctgc
      781 tggcgacaag caacgccagg cgcagccggg ccgggttcgt ccgccatgca tggagagtgc
      841 ccatcaaaac ccagccacct tcacgttgta gccgagctgc cggacgacct tcagcaggtc
      901 ctccacggca acacgcgcgt catcccgctc cacctcaatg cgcccggtcg caaaatgcac
      961 ccgggcacgc tcaacacccg gcagcgcggt tagcgcgcgc tcgagcttga cggcgcacga
     1021 ggggcagttc aggtcggcgc cacgcagtac ggtcttcatc gggttggtct ccgttcggta
     1081 ggtccaccgt tcatgaactc accgtagcgg agcgcgaccc gcgcgtgaat gatccagatc
     1141 acttaagtgc gcgtggggga atgcgagggc gaatcagccc gcgtcgccct ccgcgatggc
     1201 ggccagctgg tcgaggtcga tcagtccgat ccagcctcgg ccgctgtgga tcacgccgtc
     1261 gcgatcgagc tggctgatca cgcggctggc agtctcgcgg gtggtgccga ccatatcggc
     1321 cagatcctgg cgcgagagcg gcagttggat gacgagcatg ccctcgcgcc gctcgcccgc
     1381 gcgattggcg agcctgagca gcgtcatggc gatccgctgc tcgacatcat gcccgctgag
     1441 cccctcgatc aagtcgtggg cggtccgcag ccgctcgctg acgatgccca gggtcgtcag
     1501 cgcggccagc gggtagcgct ggagaatctg atggaacgcc gccgcgtcca cggcgagcac
     1561 gcagcaggtc gtcagcgcct cggccgagtc cgggtagacc cgatcgccca gcgtggagag
     1621 actgccgaag aagtcgccct gccccaggat atcgaggagc acgttctgcc cgctgggcat
     1681 ggggcgcagg agcttgacct gaccggcggc gacgacgtag agccagcgag cgggctctcc
     1741 gccggtgtag atcgtctggc cttcgctgaa gctgcgctca tggaaatcct ggctgatgcg
     1801 cgcgatgtcg tccgccgtga gctcggcgaa gaagggtagc cgcgccagca agtgcagccg
     1861 cagctcaagg gtgcagctcc gcgactcaat cggcttcgtg cggatccggg tcatgcgggg
     1921 attgctcacg atcgtcctgc tatccgagct cccaggcggc gaagctcgcc gggaaatgcg
     1981 gcgccgcgag ttggcgcacg gcgagggcgt aggcgcggat ctcg
//
LOCUS       DASUBI010000050         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_352911, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000050 DASUBI010000000
VERSION     DASUBI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352911"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU72_00840"
     CDS             <1..478
                     /locus_tag="VFU72_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEU5422061.1"
                     /translation="QGRPIEEPEAVSLDLPIDAVIPESYAGSEEIRIELYRRLAAVRS
                     YGELRDIQEELIDRFGPMPDSLIRLVELARLRIRASTLGITSIVEREGEVYIRPVVGS
                     KLDQRALRRELGDGVTVTPNQVRLTLNRLHADVWAAVTQVVHAIEDAEATVLAVAG"
     gene            complement(642..2999)
                     /locus_tag="VFU72_00845"
     CDS             complement(642..2999)
                     /locus_tag="VFU72_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU5422062.1"
                     /translation="MPAEIARILEFDRVLERLAGRCQFSLAAARAREIGPSADDEQVR
                     FSQAVTVEAVALLTESPGFTVGGARDVRQIVERAALGLLLAPADLRDTLDTVQASRSL
                     RRSFTQKPNAAGEFPLLNDLVEAIADLPALEADLVRTVGSRGEILDSASPELGEIRRE
                     VKLAHRRLTERLNRMIGDGEHAAAIQDPIVTMREGRYVIPVRADRRGQVPGIVHATSA
                     SGQTLFVEPLAVVELNNRWRELQMAEEHEVERVLRTRSQQIGAAAEELTRTLEAIAAL
                     DLALAKARLAFDMRATPPVIAERPPQRVALRQARHPLLDPATVVPVDLTLGGDYRVLV
                     VTGPNTGGKTVALKTLGLLAMMAQSGLFIPAADGSTLSVFDGIFADIGDEQSIEQSLS
                     TFSSHMTKIIAMLRAASERSLLLIDELAAGTDPEEGAALARAIISVLLERGALAVVTT
                     HYSELKAVAYATPGVENASAEFNLKTLAPTYRLITGIPGRSNALAIAQRLGLPAPIIE
                     EARGYLQPEAVRTDELLAEIQRRRAAAEATEREARAERAEVTRLRQEAEEALREAQRA
                     KVEARQDVLAGLEAELGQARGALRRVQSMAARPAAVVAPEEANQAGHDLQAAERQLRE
                     AVRRRHKLPVPTEQLALGDWVEVPSLGISGEIVGFGGDGESAEIQAGAFKLTQPLGAL
                     RRTAPAEKAPVRQHRPLPRLERVDQELHLRGMRVHEIEDAVDRYLDSAALASLPWVRL
                     VHGKGTGALRSAVHDALKRHPAVARFELADVEQGGSGVTVVHLKE"
     gene            complement(3334..>3621)
                     /locus_tag="VFU72_00850"
     CDS             complement(3334..>3621)
                     /locus_tag="VFU72_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422063.1"
                     /translation="LRTRITSDSRGGIALSLVLVALSAVLAIGVVTVMAQASERTIVD
                     LQPNGTVVMIDHQFRDSLLAGQVRRLTPYLYEGVGDILHTTAPQILLPVGR"
BASE COUNT          542 a         1275 c         1256 g          548 t
ORIGIN      
        1 ccaggggcgg ccgatcgagg agccggaggc ggtctcgctc gacctgccga tcgacgcggt
       61 catccctgag agctacgccg gctcggagga gattcgcatc gagctgtatc gccggctggc
      121 ggcggtgcgc tcgtacggcg aacttcgcga tatccaggag gaactgatcg accgcttcgg
      181 tccaatgccc gactcgctga tccggctggt ggagctggcc cggctgcgta tccgcgccag
      241 cacgctcggc atcacctcga tcgtcgagcg cgagggcgaa gtctacatcc ggccggtggt
      301 gggctccaag ctcgaccagc gcgcgctgcg ccgcgagctg ggcgacggcg tcaccgtcac
      361 cccgaatcag gtgcggctaa ccctgaatag gctgcacgcc gacgtctggg cggcagtcac
      421 ccaggtcgtg cacgccatcg aggacgccga ggcgaccgtc ctggcggtgg cggggtagag
      481 tcgaacggcc gcggcctttc ccgaggcatt cacggagccg aaggaaggca gacacggagg
      541 tctgccccta ccagatgcag taagagtgtc catcttgtag gatcggagcc ctgagtccgc
      601 cctgttgccg ttgcctcgcg cgtgccgtcg gtggacgacg gtcattcctt gagatgcacg
      661 accgtgacgc cggagccgcc ctgctccacg tcggctagct cgaagcgagc taccgccgga
      721 tgccgcttga gcgcgtcgtg cacggccgag cgcagggcgc cggttccctt gccatggacc
      781 agccgtaccc aggggagcga ggccagcgcg gcggagtcga ggtagcgatc gaccgcgtcc
      841 tcgatctcgt ggacgcgcat gccgcgcagg tgcagctcct gatcgacccg ctccagccgg
      901 ggcagcgggc ggtgctggcg caccggagcc ttctcggccg gcgcggtccg gcgcagcgcc
      961 cccagcggct gggtcagttt gaaggccccc gcctgaatct cggccgactc gccgtcaccg
     1021 ccgaagccga cgatctcgcc gctgatgccg agggacggca cctcgaccca gtcgccgagc
     1081 gccagttgct cggtcggcac cggcaacttg tggcggcggc gcacggcctc gcgcagttgc
     1141 cgctcggcgg cctgcaggtc gtgaccagcc tgattggcct cctcgggcgc gaccaccgcc
     1201 gccggacgcg ccgccatcga ctgcacgcgc cgcagcgccc cacgcgcctg gcccagctcg
     1261 gcctccagac cggccagcac gtcctgccgc gcctcgacct tggcgcgctg cgcttcgcgc
     1321 agcgcctcct cagcctcctg gcgcaggcgc gtcacctcgg cgcgctcggc ccgcgcttcg
     1381 cgctccgtcg cctcggccgc cgcgcgccgt cgctggatct ccgccagcag ttcgtcggtg
     1441 cgcactgctt ccggctgcag gtagccgcgc gcctcctcga tgatcggcgc cggcagccca
     1501 agccgctgcg cgatcgccag cgcgttggag cggccgggga tgccggtgat caggcggtag
     1561 gtcggcgcca gcgtcttgag attgaactcg gcgctggcgt tctcgacgcc gggcgtggca
     1621 taggccaccg ctttcagttc ggagtagtgc gtcgtcacca ccgccagcgc gccgcgctcc
     1681 agcagcacgc tgatgatggc gcgggccaac gcggcgcctt cctcgggatc ggtgccggcg
     1741 gccagctcgt cgatcaagag caagctgcgc tcgctcgccg cgcgcagcat ggcgatgatc
     1801 ttggtcatgt gggacgaaaa ggtcgacagg ctctgctcga tgctctgctc gtcgccgatg
     1861 tcggcaaaga tgccgtcgaa gacgctcagg gtcgagccat cggcggccgg gatgaagagg
     1921 ccggactgcg ccatcatcgc cagcaggccg agcgtcttga gcgccacggt cttgccaccc
     1981 gtgttcggtc cggtgaccac cagcacgcgg tagtcgcccc caagcgtcag atcgaccggg
     2041 acgaccgtgg ccgggtcgag cagcgggtga cgcgcctggc gcagggctac gcgctgcggc
     2101 ggccgctccg cgatcaccgg cggggtggcg cgcatatcga aggccagccg cgccttggcc
     2161 agcgccaggt cgagcgcggc gatcgcctcc agcgtgcgcg tcagttcctc ggccgcggcg
     2221 ccgatctgct ggctgcgggt gcgcagcacg cgctcgacct cgtgctcctc ggccatctgc
     2281 agctcgcgcc agcggttgtt cagctcgacc accgccagcg gttcgacgaa gagcgtctgg
     2341 ccactggccg aggtagcgtg cacgatgccc ggcacctggc cgcgccgatc ggcgcgcacc
     2401 gggatcacgt agcgtccctc acgcatagtc acgatcgggt cctgaatggc cgccgcgtgc
     2461 tcgccgtcgc cgatcatgcg gttgagacgc tcggtcagcc ggcggtgcgc gagcttcacc
     2521 tcgcggcgga tctcgcccaa ctccggcgag gcgctatcca ggatctcgcc tcgggagccg
     2581 acggtgcgta caagatccgc ctccagtgct ggcaggtcgg cgatcgcttc aaccaggtcg
     2641 ttgagcagcg ggaactcgcc ggccgcgttc ggcttctggg taaagctgcg ccgcagcgag
     2701 cgcgacgcct ggaccgtgtc gagggtgtcg cgcaggtcgg cgggcgcgag cagcaagccg
     2761 agcgcggcgc gctcgacgat ctggcgcaca tcccgcgcgc cgccgacggt gaagccgggg
     2821 ctctcggtca agagcgccac cgcctcgacc gtcacggcct gcgaaaagcg cacctgctcg
     2881 tcgtcagcgg acgggccgat ctcacgggcg cgcgcggccg ccagcgagaa ctggcagcgc
     2941 cccgccagtc gctcgagcac ccgatcaaat tccaatatac gagcgatctc cgccggcacg
     3001 cgagaggtcc cttgctacgg cacggcgcct cacgcatggc gcctgatcca aacctgttaa
     3061 ttgtaccgag gtcggacgag ggttgcccac caacgtgttc ggtggctacg gaattgatcg
     3121 agaggcgtct aaggacggat ccttcgactg cgctcgggat gactgaggta ggggatgagg
     3181 ctgacgacga ggccggtcgt tgtccgacgc cgatcgcatc cgtcggcccg ccattatccc
     3241 tgtcatcccg agcgcagccg aaggatccgt cgtggagcga caccacggcc actcgagaag
     3301 ccgaatcgcc cggctaagcg tacgttcccc gcgctatcgt cctaccggaa gcaggatctg
     3361 cggagcggtg gtgtgcagga tgtcacccac gccctcgtag aggtagggcg tcaggcggcg
     3421 gacctgaccg gccagcagcg agtcgcggaa ctgatgatcg atcatcacga ccgtgccatt
     3481 cggttgcaga tcgacgatgg tacgctccga ggcctgggcc attaccgtca cgacgccgat
     3541 ggcgagcacg gccgagagcg cgaccagcac caggctgagt gcgatcccac cgcggctgtc
     3601 gctggtgatc cgggtgcgca g
//
LOCUS       DASUBI010000051         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_243739, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000051 DASUBI010000000
VERSION     DASUBI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243739"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFU72_00855"
     CDS             complement(<1..689)
                     /locus_tag="VFU72_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU5422064.1"
                     /translation="MNADRNFVEELSDYEDDFDQWYVEVVRKAELADESPVRGTRVIR
                     PYGFALWENMQAALDRRIKDTGVQNAYFPLFIPKSYLEREAEHIEGFAPEVAWVTRGG
                     HKDLEEPLAVRPTSEAIICPMYARWVQSYRDLPILINQWCSVVRWEERPRAFLRGLEF
                     LWQEGHTAHASLEEAEERARLMLEVYRDFVETELAVPVVAGAKTESEKFAGALRTYTI
                     EAMMGGKKWAL"
     gene            975..2033
                     /locus_tag="VFU72_00860"
     CDS             975..2033
                     /locus_tag="VFU72_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5422065.1"
                     /translation="MRAGGHDNPDDAPLPLGKLPAALLARLLARVPDSPDVLIPAGIG
                     RDAAAVRVGDQVLVLKSDPVTFATDAVGWYAANVGANDIACLGAAPRWLLATVLLPER
                     GATPALAERILDDLLAACRSLGISLVGGHTEITAGLERPIVVGMLAGVTDPASLIDPR
                     RATAGDVILLANGIAIEGTALLARELGGPLTEHFGADFVARCREFLYQPGISVVRAAG
                     VLHGALGQAIHALHDPTEGGLATGLGELAAASDGGLIVEDDAILVYPETRAICHALAL
                     DPLGLIASGALLAVVEPTAVESALSALHAAGIHAAAIGRLTAKAGHLLLRRGGREEAL
                     PEFRVDELARYFAARGAS"
     gene            2100..2627
                     /locus_tag="VFU72_00865"
     CDS             2100..2627
                     /locus_tag="VFU72_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEU5422066.1"
                     /translation="MSFQHHEYDLLTVLDAEAIGEPGQRRFRLIVGTPGDIISLWMEK
                     EQLSALGVAIEQLLEQLREARLIHGAVEPEPAPSPTAVPLGGSEYLVSKMAIGFDEER
                     TLVALFAHDVEQEDDGEPIFAGRATLTNAKALADRIVEVVAAGRPRCPRCGAPIGPEG
                     HVCPHENGHLPWPRE"
     gene            complement(2840..2910)
                     /locus_tag="VFU72_00870"
     tRNA            complement(2840..2910)
                     /locus_tag="VFU72_00870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2876..2878),aa:Gln,seq:ttg)
     gene            3174..4250
                     /locus_tag="VFU72_00875"
     CDS             3174..4250
                     /locus_tag="VFU72_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5422067.1"
                     /translation="MAVPVTQTKEFLSRLEALAEECRAELTEVSQSLQEIQLLLTQSQ
                     SEVDRLTQREMALNNRQREMESNIESYSRRDIKDMYHASHEVELRLMMMRGQVEQLQE
                     RQTSITLYQEKLRLLLELADTQLQVEHERNSAQDTRTRMLRRGPAEIPFAEVIQAQED
                     ERLRVAQLVVDGPAQALANIVLRTEICERSYERTPETLPTELAELRRLSTNALRDTRR
                     LLYELRPVVLRELGVVPTLRRYLNDLSRLRQVPAAINGPEADDKLPELLRVVFYRLVQ
                     QMISAAAADDDVTEIDVVVRYEDAQVLARVEVYGPDRARAQAIPRLLKDETVRRRIDQ
                     LTADLHYELTGAEAAHLTLVIPLA"
     gene            4477..4659
                     /locus_tag="VFU72_00880"
     CDS             4477..4659
                     /locus_tag="VFU72_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5422068.1"
                     /translation="MAQYLKALIGMYLPETTEGQGMVEYGLILVLVSVVVITILTTMG
                     GTLNTVFTTVNTALGG"
BASE COUNT          791 a         1683 c         1596 g          764 t
ORIGIN      
        1 tgcagcgccc atttcttacc gcccatcatg gcttcgatgg tgtaggtgcg cagcgccccg
       61 gcgaacttct cggactcggt cttcgcgccg gccaccaccg gcaccgccag ctcggtctcc
      121 acgaaatcgc ggtagacctc cagcatcagc cgggcacgtt cctccgcctc ctcaagactg
      181 gcgtgggccg tgtgcccctc ctgccagaga aactccaacc cgcgcaggaa ggcgcgcggg
      241 cgctcttccc agcgcaccac gctgcaccac tgattgatca ggatcggcag gtctcggtag
      301 gactgcaccc agcgggcgta catcgggcag atgatcgcct cggaggtcgg gcgcaccgcc
      361 agcggctcct ccagatcctt gtgcccgccg cgcgtgaccc aggcgacctc cggcgcgaag
      421 ccctcgatgt gctcggcctc ccgctccaga tagctcttcg ggatgaaaag tgggaagtag
      481 gcgttctgca cgccggtgtc cttgatgcgg cgatcgagcg ccgcctgcat gttctcccag
      541 agggcgaagc cgtacggccg aatgacgcgg gtgccgcgca ccggcgactc atcggccagc
      601 tccgccttgc gtacgacctc gacgtaccac tggtcgaagt cgtcctcgta gtcgctcagt
      661 tcctcgacga agttgcggtc ggcattcatg gaagcccctc cgtgacaggc gtgtgcgggc
      721 ggaagcagcc accgcccgca ggctcaggac agcgtcaggc gcgctcggcc acgcgtcgcg
      781 ccgagcgcgc gggaggggcg ggaagcccgc cgcgccctgt gccgttgtcc tgccggaggg
      841 tcatctgcct gactccatcc tgcgccgccc gccactcggg cgcgcggcga cggagtgtag
      901 aatgccagga gcgtccggcc cctggcgagc agcaccaaag tataccagtg ccgcgggatg
      961 agttcggaga cgtgatgcgg gcaggcgggc acgacaaccc agacgacgcg ccgcttccgc
     1021 tcggcaagct gcccgccgcg ctgctggccc ggcttttggc gcgcgtcccg gacagcccgg
     1081 acgtcctcat accggctggc atcgggcgcg acgccgccgc agtgcgcgtc ggggaccagg
     1141 tgctggtgct caagtccgac cccgtcacct ttgccaccga cgccgtcggc tggtacgcgg
     1201 ccaacgtcgg cgccaacgac atcgcctgcc tcggcgccgc gccgcgctgg ctgctggcga
     1261 ccgtgctgct gcccgagcgc ggcgccactc cggccctggc cgagcgcatc ctcgatgacc
     1321 tgctggccgc ctgtcgctcg ctgggaatca gcctggtcgg cggccacacc gagatcaccg
     1381 ccgggctcga acgcccgatc gtggtcggca tgctggccgg cgtcaccgac ccggcgagct
     1441 tgatcgaccc gcgccgcgcc acagccggcg acgtcatcct gctcgccaac ggcatcgcga
     1501 tcgagggcac cgcactgctg gcgcgtgagc tcggcggtcc gctgaccgag catttcggtg
     1561 cggacttcgt ggcgcgctgc cgcgagttcc tctaccagcc cggcatcagc gtggtcaggg
     1621 ccgccggcgt gctccacggc gccctgggcc aggccatcca cgcgctgcac gacccgaccg
     1681 agggcgggct tgcgaccgga ctcggcgagc tggcggccgc gagcgacggc gggctgatcg
     1741 tggaggacga cgccattctc gtgtacccgg agacgcgcgc catctgccac gcgctggcgc
     1801 tcgatccgct gggcctgatc gcctctggcg cgctgctcgc ggtcgtcgag ccgacggcgg
     1861 tcgaatcagc gttgtccgcg ctgcatgccg cggggatcca cgccgcggcg atcggccgcc
     1921 tgaccgccaa ggcaggacat ctgctgctgc gccggggtgg gcgcgaggag gcgctgccgg
     1981 agttcagagt cgatgaactg gcgcgctact tcgccgcgcg tggagcgtcc tgaacgggtc
     2041 gcgttgcgcg tgtagtatgc tggtagcggt agtcgcgggg cgggagagga gcagaggcgg
     2101 tgtcgtttca gcaccatgag tacgatctac tgaccgtgct cgacgccgag gcgatcggcg
     2161 agcccgggca gcgccgcttt cgcctgatcg tcggaacccc cggcgacatc atctcgctct
     2221 ggatggagaa ggagcagctc agcgcgctcg gcgtcgcgat cgagcagttg ctcgagcagc
     2281 tgcgcgaggc caggctgatc cacggcgccg tggagccgga gccggcgcct tcccccaccg
     2341 cggtaccgct cggcggctcc gagtacctgg tcagcaagat ggccatcggc ttcgacgagg
     2401 agcgcacgct ggtcgccctt tttgcccacg acgtggagca ggaggacgat ggcgagccga
     2461 tctttgccgg gcgagcaacc ctgaccaacg ccaaggcgct ggccgaccgc atcgtcgagg
     2521 tggtcgcagc cggccgcccg cgctgcccgc gctgcggcgc ccccatcggc cccgagggtc
     2581 acgtctgccc ccacgagaac ggccacctcc cctggccgcg ggagtgagcc gcgcggccgg
     2641 cacgtccgca cggcagcttc ccaacgcgat tagcgccggt ggcgcgcgtc gttggcatcg
     2701 atggtgtcgt cgctggagtg tgacgaaccc ttagcctcct gaagcaggcg tcccatacgc
     2761 acgcggtggc ttcagctagg acggagccgc ggtgccacga agatcacccg atggattgtg
     2821 acagagggtg cgtcgagggt tgccgggcca ggattcgaac ctggaaaaga ggctccaaag
     2881 gcccctgtga taccgtttca ccacccggca gcgcgcggtc ggcaggcgag cgcgccgtgc
     2941 ttaattgtag tttccgcggg cacccccagt caaatgaagc ccctcaggcg caactccagc
     3001 ccctgaactg aacgttaccc gtccagcacc gagtcaacgc agctagggca ttcggccccc
     3061 ctcatctttc ggccaggctt gggactccag taccgttgcc gccgggaggt tccgctccta
     3121 accttacaac catacgtcgg ttgcggatca tcttcgccga acggagacga tgcatggcag
     3181 tcccggtgac ccaaaccaaa gagttcctca gccgcctcga agcgctggcc gaggagtgcc
     3241 gtgccgaatt gaccgaggtg tcccagtcgc tgcaggagat tcaactgctg ctgacccagt
     3301 cgcagagcga ggtcgaccgg ctaacacagc gcgagatggc gctcaacaac cggcagcgcg
     3361 agatggagag caatatcgag tcctactcgc gccgcgacat caaggacatg tatcacgcca
     3421 gccatgaggt cgagctgcgc ttgatgatga tgcgcgggca ggtcgagcag ctccaggagc
     3481 ggcagaccag catcacgctc taccaggaga agcttcgtct cctactggag ttggccgaca
     3541 cccagctgca agtcgagcac gaacgcaata gcgctcagga tacccgcaca cgcatgctgc
     3601 ggcgcgggcc agccgagatc cccttcgccg aggtgatcca ggcccaggag gatgagcgcc
     3661 tgcgcgtggc gcagttggtc gtcgatggtc cggcccaggc gctggccaac attgtcctcc
     3721 gcaccgagat ctgtgagcgc agctacgagc gtacccccga gacgctgccc accgagctgg
     3781 ccgagctgcg ccggctctcc accaacgccc tgcgcgacac acggcgcctc ctctacgagc
     3841 tgcgtccggt cgtgctgcgc gagctgggtg tcgtgcccac gctccgccgc tacctgaacg
     3901 acctgagccg gctgcgccag gtgcctgccg ccatcaacgg tcccgaggcg gacgacaagc
     3961 tccccgaact gttgcgcgtc gtcttctacc ggctggtcca gcagatgatc tcggccgccg
     4021 cggccgacga cgacgtgacc gagatcgacg tggtagttcg ctacgaagac gcgcaggtgc
     4081 tggcccgcgt ggaggtctac ggcccagacc gcgcccgcgc ccaggcgatc ccgcgcctgt
     4141 tgaaggacga aacggtgcgt cgccgcatcg accagctcac cgccgacctg cactacgagc
     4201 taaccggcgc cgaggccgcg cacctgacgc tggttatccc tctcgcgtaa ccccccagtc
     4261 gggactcgcg caccgagcaa cctggacgag tggttcattg agaccccgcg ccccgcaccc
     4321 taagctaaca gcataacaac gtgatcctcg atggccgccg cgaggcggcc ccggagcgag
     4381 accggacgag cggaaggagg gaacggggcg tccgggtggg aaccgatcgg ctgattaata
     4441 ccgtcattgt tcatcattgt ggagggaaca cacatcatgg cacagtacct taaggcgctg
     4501 atcggcatgt atctgcccga gaccaccgag ggccagggca tggtcgagta cggtctgatc
     4561 ctggtgctcg tttcggtcgt cgtcatcacc atcctgacca ccatgggcgg gactctcaat
     4621 accgtgttca cgacggtcaa taccgcgctc ggcggctaat cggttcagga ctcgaacgac
     4681 ggccgttctc cgagcgtgag aacggccgtt gctcttgccg ggcgcatgag aagggtcggg
     4741 tctgtgctga agcatctctg ccaccccaca agtctcgatg ctcaagggct tgtcgagtac
     4801 ggcttcatct tgctgcttgt ggctatggtg gtcg
//
LOCUS       DASUBI010000052         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_216031, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000052 DASUBI010000000
VERSION     DASUBI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216031"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFU72_00885"
     CDS             <1..239
                     /locus_tag="VFU72_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEU5422069.1"
                     /translation="DAYLAIGLAPLLTLSGAGLALLVACPRRAGQVLTFLVGNAAGAT
                     GDLWVIRRVAALPADARLRDLADGFAVYLPDDGG"
     gene            complement(529..2481)
                     /locus_tag="VFU72_00890"
     CDS             complement(529..2481)
                     /locus_tag="VFU72_00890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5422070.1"
                     /translation="MADEQNNRLSFEQAVSATMVTDVRLSPDGRQVAIVTTPASKAGE
                     FPESTIWLVSAEGGSPRRLTGAEAANGGPRWSPDGRRIAFVSDRRQRGLPQLYVVELG
                     GGEAVRLTDAAGGVAAAAWSPDGKTLAFSAMDAETDEEKKRREERNDHKVMDADIKRA
                     SLWLLEVPADAAALAPDDLPEARRISPEGMHIGAQGDAGFAWAPDGTRLVVTGTPSPK
                     AHYTFSPEMLLITRDGEVTNLGRFEGLVGAPEFSPDGRTLAFNAAEGVIPALFSLQTM
                     PVSGGEPHIVAPNLEGSFQSFAWLPDGQRIVAGVEIKQSSLLKLVDVAGGQLSDAIEP
                     FAEPGITALLSLSADGTRVAFAHASDYAHADVYVAELGGQPRKLTDLNPWTREQQWGE
                     VREVRWRSKDGMEIEGMVILPVGYQEGQRAPLLLHIHGGPCGAWTHALQATWHNWGQF
                     LAQRGYAVFLPNPRGSSGRGTKFLSEIVGCYGEPDWDDLMTGVDHLIESGIADPDQLV
                     VGGWSGGGFLTNWTITHSNRFKAAVSGAGISNWVSFQGTADVRSVFDSYLGRVDEEPE
                     VHWRLSPIRNIKHASTPTLILYGEADARVPVTQGYELYEGLKGRGVETELVAYPREPH
                     SIGERKHQLDLLPRAIGWYQRHLGRE"
     gene            2651..2869
                     /locus_tag="VFU72_00895"
     CDS             2651..2869
                     /locus_tag="VFU72_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422071.1"
                     /translation="MVDPRDEAAETLERLAAELELAARHCRTAAGHYRQREVPRASAH
                     LLAAHGHLHRAQHGLDTLAEQHADHSSP"
BASE COUNT          411 a         1059 c         1075 g          473 t
ORIGIN      
        1 gcgatgccta cctggcgatc ggcctggcgc cactgctgac gctctcgggg gccgggctgg
       61 cgctgctggt ggcgtgcccg cggcgcgccg ggcaggtgct gaccttcctg gtgggcaacg
      121 cggccggcgc gaccggcgat ctctgggtca tccggcgcgt ggcggcgctc cccgccgacg
      181 cgcggctgcg cgacctggcc gatggattcg cggtgtatct gccggatgat ggtggctgac
      241 gacgacgctg gggcgctcgt gaggacggat ccttcgactt cgctcaggat gacagggtca
      301 aagaggggcg cacgacgacg atagacctcg tccggtaggc ctctccttct ctccgtcatc
      361 ctgcgcggag tcgagagatc cgtgcttacg cggcacccaa cgaagagggc gggtttgaaa
      421 cccgccccta caatcgttcc tgctcccttc tcccgcgcat cggctccgtc cggagaaggg
      481 ttggggatga gggctcccct gggcgcgggg tgagggcaac cccgcgccct actcccgccc
      541 caggtgccgc tggtaccagc caatggcgcg cggcaggaga tcgagctggt gcttgcgctc
      601 gccgatcgag tgcggctcgc gcgggtaggc gaccagctcc gtctcgaccc cgcgcccctt
      661 cagcccctcg tagagctcgt agccctgcgt caccggcacg cgcgcgtcgg cctcgccgta
      721 gaggatcagc gtgggcgtgc tggcgtgctt gatgttgcgg atcggcgaga ggcgccagtg
      781 cacctccggc tcctcgtcga cgcggcccag gtagctgtcg aagacgctgc gcacgtccgc
      841 cgtgccctgg aagctgaccc agttcgagat gcccgcgccg gagacggccg ccttgaagcg
      901 gttgctgtgg gtgatggtcc agttggtcag gaagccgccg ccgctccagc cgccgacgac
      961 gagctgatcc gggtcggcga tgccgctttc gatcaggtga tccacgccgg tcatcaggtc
     1021 gtcccagtcc ggctcgccgt agcaacccac gatctcgctg aggaacttcg tgccacggcc
     1081 ggaggagccg cgcgggttgg gcaggaagac ggcgtagccg cgctgggcca ggaactgccc
     1141 ccagttgtgc caggtggcct gcagggcgtg cgtccaggcg ccgcaggggc cgccatggat
     1201 gtgcaagagc aacggggcgc gttggccctc ctggtagccg accggcagga tgaccatgcc
     1261 ctcgatctcc atgccgtcct tcgagcgcca gcgcacctcg cgcacctcac cccactgctg
     1321 ctcgcgtgtc caggggttga ggtcagtcag cttgcgcggc tggccaccca gctcggctac
     1381 gtagacatcg gcgtgggcgt agtcgctggc gtgcgcgaag gccacgcgcg tgccgtcggc
     1441 gctcaggctg aggagggcgg tgatgcccgg ctcggcgaag ggttcgatgg cgtcgctgag
     1501 ttgcccgccg gccacgtcca cgagcttgag caggctgctc tgcttgatct cgaccccggc
     1561 cacgatgcgc tggccatcgg gcagccaggc gaaggactgg aacgagccct ccaggttcgg
     1621 cgccacgata tgcggctcgc cgcccgagac cggcatcgtc tgcagcgaga agagcgccgg
     1681 aataacccct tccgcggcgt tgaaggccaa cgtgcggccg tcgggactga actccggtgc
     1741 gccgacgagc ccctcgaagc gcccgaggtt cgtcacctcg ccgtcgcgcg tgatcaagag
     1801 catctcgggc gagaaggtgt agtgcgcctt gggcgagggt gtgccggtca cgaccagcct
     1861 ggtgccgtcc ggcgcccagg cgaagccggc gtcaccctgc gcgccgatgt gcatcccttc
     1921 cgggctgatg cggcgcgcct cgggcaggtc gtcgggcgcc agcgcggccg cgtcggccgg
     1981 cacctcgagc agccagaggc tggcgcgctt gatgtcggcg tccatcacct tgtggtcgtt
     2041 gcgctcctcg cgccgcttct tctcctcgtc ggtctcggcg tccatggcgc tgaaggccag
     2101 cgtcttaccg tccggcgacc acgccgccgc cgccacgccg ccggcggcgt cggtcagtcg
     2161 cacggcctcg ccgccgccca gctcgaccac gtagagctgc ggcaggccgc gctggcgccg
     2221 gtcggagacg aaggcgatgc ggcgcccatc gggcgaccag cgcggcccgc cgttggccgc
     2281 ctcggcgccg gtcagccggc gcgggctgcc gccctccgcg ctcaccagcc agatggtgct
     2341 ctcggggaac tcgcccgcct tggaagccgg ggtggtgaca atggccacct gccggccgtc
     2401 cggcgagagg cgcacgtcgg tcaccatcgt ggcgctgacc gcctgctcga agctcaacct
     2461 gttgttctgc tcgtccgcca tcaaatctcc ttcgtccttc gcgtcacgtc caggcagctt
     2521 gctgcgcgta atcaaagctc gctcaggcta gcggttgcgt gacccatcgt caaggccgtc
     2581 gtttgaccga ggaccggggc tcagcgaagc ttggaaacgg tgagaggcga gcgttggaga
     2641 ggagcagagg atggtcgatc ctcgtgatga ggcggcggag acgctggagc ggctagcggc
     2701 agagctggag ctggccgcgc gccactgccg cacggccgcc ggccattacc gccagcgcga
     2761 ggtgccgcgc gccagcgccc acctgctcgc tgcccacggc cacctccacc gcgcccagca
     2821 cggcctcgac accctggccg agcagcacgc cgatcactcg tccccctgaa ctgcccgatc
     2881 gtccgcttga cgcgcccgcg gcggcgcgcg tagcatcacg ctatcgaccg ggatatggcg
     2941 aggggtggga gtgcaggtgg cgcggcggta cgtggtcggg cgcgtgcggg acttcccgcc
     3001 gggcgagcgg cgcatcat
//
LOCUS       DASUBI010000053         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1210622, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000053 DASUBI010000000
VERSION     DASUBI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210622"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFU72_00900"
     CDS             complement(<1..596)
                     /locus_tag="VFU72_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5422072.1"
                     /translation="MAEAVDHLLQIPLVDLRVQYAGIKEQIMEGIATALDGMNLLLGP
                     NVTAFETEFAAYCGARFAIGVANGTDALYLALRACGVRPGDEVITVSHSFIATAEAIV
                     NLGAIPVYVDVDPATYTLDPNTLEAAIGPKTRAIIPVHLYGQMADMDPIMAIAEQYGL
                     VVIEDACQAHGATYKGRGAGSIGDAAAFSFYMGKNLGA"
     gene            complement(613..1593)
                     /locus_tag="VFU72_00905"
     CDS             complement(613..1593)
                     /locus_tag="VFU72_00905"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5422073.1"
                     /translation="MSATATRVVSYRDALYEALREEMERDERVIMLGEDFANGGAFGV
                     AGDLPARFGGQRVIHTPISENSFVGVAVGAAMTGLRPVVEIMFMDFIALAMDQLANHA
                     AKIHYMYAGQYDVPLVVRTPAGAGRGYGASHSQSLESWLIQVPGLKIVAPSDPADAKG
                     LLKSAIRDDNPVVFIENKLLYPKKIEWQGDETDELVPLGVARVRREGGDVTLVSYGRM
                     LDLALEAAELLAAEGLDCEVIDLRTLKPLDTETLYRSLARTGRLVFVEEGTGGIGAEV
                     GARAAEHLPGLRLVRVAARDQPIPSSGPLEEHVIPQVTDIINGCLDSITG"
     gene            complement(1590..2609)
                     /locus_tag="VFU72_00910"
     CDS             complement(1590..2609)
                     /locus_tag="VFU72_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5422074.1"
                     /translation="METFEQYLDYYRRMQRIRQCEERIGWLFSRGLTMGTAHLSIGQE
                     AVAVGVLAAAEPTDQVVSTHRGHGHLIAKGAEPARILAEICGRASGYCRGKGGSQHIA
                     VRELDFLGTNGITGGGIPVATGAALTAKLRGTGRVVFSFFGDGAANQGTFHESLNMAA
                     VWSLPIVYVCENNGYAMYTPQHEVTSVCDIAVRARGYGIPGVTVDGMDLLALREAAVE
                     AVTRARSGQGPSLIEAKTYRLCGHSKSDTGRAYRPADEIAAWQERDPLDTWRERLVAL
                     GVDEAALTAIEDAVSAEIEAATQFALDDVYAEDEALTGIYAEPPLFAANYAHSRVAVE
                     VGGQA"
     gene            complement(2626..3807)
                     /locus_tag="VFU72_00915"
     CDS             complement(2626..3807)
                     /locus_tag="VFU72_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5422075.1"
                     /translation="MASEVILPKLGMNMETAIITSWYKQEGEPVAVGDILAEVETDKA
                     NIELEAEAAGFMRRFLVAEGEQVAVHQAIALIGALDEDITAQEAELRARTAMPAEEAH
                     KARVYAGWQDQGQGQRHGVAERAGVVPAATNGRGAPAAAPASPAATDGRLDPAAIRAR
                     LERRGVLPNGNGARPAARTRLVIYGGGLGAKQLLEVTRQCEDIEVVGLIDDNPGLHGA
                     EFGGVRVLGGFAALAELARRRELDGVALSFHSEVRKKVHERIKAELDLPFVPLVDPRA
                     AIGMDVTIEPGALVEAGAVIGPGTVVGEGTIVDVGVVVAHDCYLGAFSHLSPGCTLSG
                     VVCLQGNVLVGVGAALNSTITIGRNVIITPGAAVMNDVPDDTVVSGVPAKPIGASRRG
                     A"
     gene            complement(3843..4643)
                     /locus_tag="VFU72_00920"
     CDS             complement(3843..4643)
                     /locus_tag="VFU72_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5422076.1"
                     /translation="MRVEKPLAAKSVEAEELSALAEARGLKLMVGHTFEYNGAIRLLR
                     EIVRSGELGDIYYIDSARLNLGLFQHDVNVLWDLAPHDLSILLYVLGMEPIAVSARGS
                     SSICPGIHDVAYVELRFPGNILAHLHLSWLDPCKVRRITVVGSKKMLVCDDVSESEKI
                     RIYDKGVELPYETDQFQDFHLQYRYGSVSIPHVPMREPLRVQCEHFIHCVRTGEQPQS
                     DGRVGTQVVRIIEQADKSLHNGGEREPVRLDLYGPWQADPVMQSIVTH"
     gene            complement(4647..>4926)
                     /locus_tag="VFU72_00925"
     CDS             complement(4647..>4926)
                     /locus_tag="VFU72_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5422077.1"
                     /translation="VVSEQRSVKVGVVGFGYWGPKLVRNYSEIPNAELAWIADRDPER
                     LARVRADYPFVRATADYDEMLRSEIEAVVVATPIPSHYRLAKAALLAG"
BASE COUNT          769 a         1763 c         1594 g          800 t
ORIGIN      
        1 taggcgccca ggttcttgcc catgtagaag ctgaaggcag ccgcgtcccc gatgctgccg
       61 gcgccgcgcc ccttgtaggt cgcaccgtgc gcctgacagg catcctcgat gaccaccaga
      121 ccgtactgct cggcgatcgc catgatcggg tccatgtcgg ccatctgccc gtagaggtgc
      181 accggaatga tcgcgcgcgt cttggggccg atggcggcct ccagcgtgtt gggatcgagc
      241 gtgtaggtcg ccggatcgac atccacgtag accgggatgg cccccagatt gacgatcgcc
      301 tcggcggtcg cgatgaagga gtgcgagacg gtgatcacct cgtcgcccgg ccgcaccccg
      361 cacgcgcgca aggccaggta gagcgcgtcg gtgccgttgg cgacgccgat cgcgaaacgc
      421 gccccacagt aggcggcgaa ctccgtctcg aaggcagtca cgttcgggcc aagcaggaga
      481 ttcatgccat cgagggccgt ggcgatccct tccatgatct gttctttgat tccagcatat
      541 tgcacccgca agtcgacgag tggaatctgc aacaagtgat cgactgcctc tgccatgggg
      601 ccaacctccc acctatccgg tgatgctgtc gagacaacca ttgatgatat cggtcacctg
      661 gggaatcaca tgctcctcga gcgggcccga ggaggggatc ggctggtcgc gcgcggccac
      721 ccgcaccagg cgtaagccgg gcaggtgctc ggcggcccgc gcgccgacct ccgcgccgat
      781 gccgccggtg ccttcttcca caaagaccag gcgaccggtg cgcgccagcg agcggtagag
      841 cgtctcggtg tcgagcggct tgagcgtgcg caggtcgatc acctcgcagt cgagcccttc
      901 ggcggcgagc agctcggcag cctccagcgc gaggtcgagc atgcgcccat aggagacgag
      961 cgtgacatca ccaccttccc ggcgcacccg ggcaacaccg agcggcacca gttcatccgt
     1021 ctcatctccc tgccactcga tcttcttggg atacaggagc ttgttctcga taaagacgac
     1081 ggggttgtcg tcgcggatcg cgctcttgag caaccccttg gcgtcggcgg ggtcgctcgg
     1141 cgccacgatc ttcagccccg gcacctggat cagccacgac tccaggctct gcgagtggct
     1201 ggcgccatag ccgcgcccgg cgccggccgg cgtgcgcacg accagcggca cgtcgtactg
     1261 cccggcgtac atgtagtgga tcttcgccgc gtggttggcg agctggtcca tcgccagcgc
     1321 gatgaagtcc atgaacatga tctcaaccac cggccgcagc ccggtcatcg ccgccccgac
     1381 cgccacgccg acgaagctgt tctccgagat cggggtgtga atcacgcgct gcccgccgaa
     1441 gcgcgcgggc aggtcgccgg ccacgccgaa ggcgccaccg ttggcgaagt cctcgccaag
     1501 catgatgacc cgctcgtcgc gctccatctc ctcacgcagc gcctcataaa gagcgtcgcg
     1561 atagctgaca acccgggtgg ccgtggcgct catgcctgcc ctccaacctc aaccgcgacc
     1621 cggctgtgcg cgtagttcgc ggcgaagagc ggcggctcgg cgtagatgcc cgtcagcgcc
     1681 tcatcctcgg catagacatc gtcgagggcg aactgggtgg cggcctcgat ctcggccgag
     1741 acggcgtctt cgatggccgt cagcgccgcc tcatccacgc ccagcgccac caggcgctcg
     1801 cgccaggtgt cgagcggatc acgctcctgc caggcggcga tctcgtcggc ggggcggtag
     1861 gcccggccgg tgtcgctctt ggagtggccg cagagccggt aggtcttggc ttcgatcagg
     1921 ctcggcccct gccccgacct ggcccgcgtc accgcctcca cggccgcttc gcgcagcgcc
     1981 agcaggtcca tgccgtcgac cgtaacccct gggataccgt agccgcgcgc ccgcaccgcg
     2041 atgtcgcaga cgctggtgac ctcgtgctgc ggggtgtaca tcgcatagcc gttgttctcg
     2101 cagacataga cgatcggcag gctccagacg gctgccatgt tcagcgactc gtgaaaggtg
     2161 ccctgattgg ccgccccgtc gccgaagaac gagaagacga cccgcccggt gccgcgcagc
     2221 ttggcggtca gggccgctcc ggtggcgact gggatgcccc cgccggtgat gccgttggtg
     2281 cccagaaagt cgagctcccg caccgcgatg tgctgggagc cgcccttgcc gcggcagtag
     2341 ccggaggcgc gcccgcagat ctcggccagg atgcgcgccg gctcggcgcc cttggcgatc
     2401 aggtggccgt gcccgcggtg cgtgctgacg acctgatcgg tcggctcggc cgcggccagc
     2461 acgccgaccg ccaccgcctc ttgtccgatc gagaggtgcg ccgtgcccat cgtcaggcca
     2521 cggctgaaga gccagccgat gcgctcctca cattggcgaa tgcgctgcat ccggcgatag
     2581 tagtcgaggt actgctcgaa ggtctccacg agtgctcgct ccctttcagg cgccgcgccg
     2641 gctggcgccg atcggcttgg ccggtacgcc gctgacgacg gtgtcgtccg gcacatcgtt
     2701 catcaccgcc gcccctgggg tgatgatcac gttgcgcccg atcgtgatgg tcgagttgag
     2761 ggcggcgccc acccccacca ggacattccc ctggagacag acgacgccgg agagggtgca
     2821 gcccggcgac aggtgcgaga acgcgcccag atagcagtcg tgcgcgacca ccacgccgac
     2881 atccacgatg gtcccttcgc ccaccaccgt gcccggtccg atgaccgcgc cggcttcgac
     2941 cagcgcgccc ggctcgatgg tgacgtccat gccgatggcc gcgcgaggat caaccagcgg
     3001 cacgaacggg aggtcgagct ccgccttgat ccgctcgtgg accttcttgc gcacctctga
     3061 gtggaaggag agcgcgacgc cgtccagctc gcgccgccgc gccagctcag ccagcgccgc
     3121 gaacccgccg agcacgcgca cgccgccgaa ctcggcgccg tggagtcccg ggttgtcgtc
     3181 gatcagcccc accacctcga tgtcctcgca ctggcgcgtg acttcgagga gctgcttcgc
     3241 gcccaggccg ccgccgtaga tcaccaggcg cgtgcgcgcg gccggccgcg ctccgttgcc
     3301 gttcggcagc acgccgcgcc gctccagccg tgcccggatc gccgcggggt cgagccgtcc
     3361 atccgtggcc gcgggcgaag cgggcgcggc ggcaggcgcg ccgcggccat tggtcgcggc
     3421 gggcacgacg ccggcacgct ccgcgacacc gtgcctttgc ccctggccct ggtcctgcca
     3481 gccggcgtag acccgcgcct tatgcgcctc ctccgccggc atagccgtcc gcgcgcgcag
     3541 ctcggcttcc tgagcggtga tgtcctcgtc gagcgccccg atcagggcga tcgcctgatg
     3601 gacggcgacc tgttcgccct cggcgaccag aaaacgccgc ataaatccgg cggcttccgc
     3661 ctccagctcg atgttggcct tgtcggtctc gacctcggcc agaatgtcgc caaccgcgac
     3721 cggctcaccc tcctgcttgt accagctggt gatgatggcc gtctccatgt tcataccgag
     3781 cttgggcagg attacctcgc ttgccacaat tgatcctctc cgcgcaccgc cggccggctc
     3841 acctagtgcg tcacgatcga ctgcatgacc gggtcggcct gccacggacc atacagatcg
     3901 agccggaccg gctcgcgctc cccaccgttg tgcaacgact tgtcggcctg ctcgatgatc
     3961 cgcaccacct gggtgccgac ccgcccgtca ctctgcggct gctcgccggt gcggacgcag
     4021 tggatgaagt gctcgcactg cacgcgcagc ggctcgcgca tcggcacgtg cggaatgctg
     4081 acactcccgt agcggtactg caggtgaaaa tcctggaatt ggtcggtctc gtagggcagc
     4141 tcgactccct tatcgtagat gcggatcttc tcgctctcgg agacgtcgtc gcagaccagc
     4201 atcttcttgc tgccgaccac ggtgatccgc cgcaccttac acgggtccaa ccagctcagg
     4261 tgcaggtggg ccaggatgtt gcctggaaag cgcagctcca cgtaggccac gtcgtggata
     4321 cccggacaga tgctgctgct gccacgcgcg ctcaccgcga tcggctccat tcccagcacg
     4381 tagaggagaa tggagagatc gtggggtgca agatcccaga ggacgttgac gtcgtgctga
     4441 aagaggccga ggttgagccg ggccgagtcg atgtaataga tgtcgcccaa ctcgccgctg
     4501 cgcacgatct cgcgcagcag gcgaatcgcg ccattgtatt cgaaggtgtg cccgaccatg
     4561 agcttcagac cgcgcgcctc ggccagcgcg ctcaactcct ccgcctcgac gcttttggcc
     4621 gccagcggct tctcgacccg cacatgctag ccggccagca acgcggcctt cgccaggcgg
     4681 tagtggctgg ggatcggcgt cgcgaccacc acggcctcga tctcggagcg cagcatctcg
     4741 tcgtagtcgg cggtcgcgcg cacgaaggga tagtccgcac gcacacgcgc cagccgctcc
     4801 gggtcgcggt cggcgatcca ggccagctcc gcgttgggga tctcgctgta gttgcgcacg
     4861 agcttgggtc cccaatagcc gaagccgaca accccgacct tgaccgagcg ctgctcgctc
     4921 acgacc
//
LOCUS       DASUBI010000054         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_66292, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000054 DASUBI010000000
VERSION     DASUBI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66292"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFU72_00930"
     CDS             complement(<1..417)
                     /locus_tag="VFU72_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEU5422078.1"
                     /translation="MNTARQAATIAAAPRTLAITAGVCYLLTHVTSVSAPFLYGPVLH
                     EARYIAGAGPATRVLVGAFCEVILALAIVGTAVALYPVVRRHHEGLALGYVGLRTLEA
                     AIIAAGIVPLLAVVTLRQQLAGATGADAATLIPLGQA"
     gene            complement(557..1246)
                     /locus_tag="VFU72_00935"
     CDS             complement(557..1246)
                     /locus_tag="VFU72_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422079.1"
                     /translation="MKPTSAQIIRGTGLAALAAGVIFAAIQPIHPPDVGASVTTTAWA
                     IITPLKWGMCLLFLVGIGGLYARQVEESGWLGLAGALLLGLSWALQTGFVFAEAFVAP
                     PLAATSPGFVDALMGISYGHTGGFALGALPALYSVMGILYMLGGLVFGLATLRAGVLP
                     RWPAGLLAAVSAVTPAAALLPHHLQRLVGMPVGLALAWLGVALWAERRAHAAESAPGS
                     SSLQVSQTGAD"
     gene            complement(1306..1623)
                     /locus_tag="VFU72_00940"
     CDS             complement(1306..1623)
                     /locus_tag="VFU72_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422080.1"
                     /translation="MARRERGVMGTPHDATSDEHRPDWYEIRLQGHLGDRWAAWFDGL
                     ALTREGDGTTRLCGPVADQAALHGLLQRVRDLGLPLLAVRKVKPEQASEAAGDADTNH
                     DAP"
     gene            complement(1843..>3107)
                     /locus_tag="VFU72_00945"
     CDS             complement(1843..>3107)
                     /locus_tag="VFU72_00945"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5422081.1"
                     /translation="AHHARRVLEVAAEDDQLSRGGAAGFLGLAAWARGELETAHRTFA
                     AGLALLQQAGSSADVVGGTLALAEIRMTQGQLREAARTYEGALRLAAEQGAPALRGTA
                     DMHVGLAELHREWGDLDAATRHLRRAEEQGEHTGFPQHPYRRRIAAARLQEAQGDPAG
                     ALALLDEAARVYVGDFYPEVRPIAALVARVWLTQGRLDDAEDWARERGLSADDDLSYL
                     REYEHLTLARVLLARHARDRAEGTVREALGFLARLLDAAEAGGRSGSVIETLALQALA
                     HRTRGDLPAALASLGRALALAEPEGYIRLFVDEGPPMADLLEMAARRGIAPDYPRRLL
                     AAFGGARERTPAKQDLVEPLSERERAVLRLLATDLGGPAIAHELLISLNTLRTHTKNI
                     FGKLGVNNRRAAVRRAEELQLLSRTRTG"
BASE COUNT          401 a         1098 c         1197 g          411 t
ORIGIN      
        1 ggcctgcccg agggggatga gggtggcggc gtcggcgccg gtcgcccccg ccagctgctg
       61 ccgcagcgtc acgacggcga ggagcgggac gatgccggcg gcgatgatgg cggcctcgag
      121 ggtgcgcagg ccgacgtagc cgagggcgag accctcgtgg tgccgccgga cgacggggta
      181 gagcgcgact gcggtgccaa caatggcgag cgccaggatg acctcgcaga acgcccccac
      241 cagcacccgg gtggcggggc ccgcgccggc gatgtaccgg gcctcgtgca gcaccgggcc
      301 gtacaggaac ggcgcgctga ccgaggtaac gtgggtgagg aggtagcaga cgcccgccgt
      361 gatggcgagg gtccgtggtg cggccgcgat ggtggccgcc tgccgcgcgg tgttcatcag
      421 ttcggctccc ttcatggttc cggcccgcgc cagcaaacgg ggtcgtgcgg cagtgctggg
      481 tgcgttgcgc agcaggttcg ggcgctggca ggccgccagg tggccgcctg cccaggccgt
      541 gccgtggtcg gcgaacttag tccgcgccgg tctggctgac ctggaggctg ctgctaccgg
      601 gcgcggactc cgcggcgtgg gcgcgccgct cggcccagag cgcgacgccc agccaggcca
      661 gcgccagccc caccggcatc cccacgagcc gctggaggtg gtgcgggagc agcgccgccg
      721 ccggggtcac cgccgacacg gcggcgagca ggccggccgg ccagcgcggc aggacgccgg
      781 cgcggagcgt ggcgaggccg aacaccaggc cgccgagcat atagagaatg cccatgacgc
      841 tatagagcgc cgggagtgcg ccgagggcga acccaccggt gtgcccgtag gagatcccca
      901 tgagggcatc gacgaacccc ggcgacgtgg ccgccagcgg cggcgcgacg aaggcctcgg
      961 cgaagacgaa gcccgtctgg agcgcccagg agaggcccag caggagcgcg ccggccaggc
     1021 cgagccaccc ggactcctcc acctggcggg cgtagagccc gccgatgccg acgaggaaga
     1081 ggaggcacat accccacttc aagggggtga tgatcgccca ggcggtggtt gtgaccgacg
     1141 ccccgacgtc gggcgggtgg atcggctgga tggccgcgaa gatgaccccc gccgccaggg
     1201 cggccaggcc ggtgccacgg atgatctgcg cgctggttgg cttcatcggt tcgtctcctt
     1261 tgcttgtcgc cgttatcgcg gggtgtgccg caccgtgacc aggcgtcacg gcgcatcgtg
     1321 gttggtgtcc gcatcgcccg ccgcctcgct cgcctgctcg ggcttgacct tcctgactgc
     1381 gagcagcggc aggccgaggt cgcgcacgcg ctggagcagg ccgtgcagcg cagcctggtc
     1441 ggcgacggga ccgcagaggc gggtggtgcc gtcgccctcg cgggtgaggg cgagcccgtc
     1501 gaaccaggcg gcccagcggt cgccgaggtg cccctggagg cggatctcgt accagtcggg
     1561 ccggtgctcg tcgctagttg cgtcgtgcgg tgtgcccatc accccccttt ctcgccgtgc
     1621 catcggcctg tcctccgccg tcgttccgta gttcactggg ctgatcagac gatagacctg
     1681 gacgtggtga ggcggcatca ccagatgtgg tgagtggggt ggtgattttg cgcgcgggtg
     1741 ccagtggggg gcgcggcggg cagagttggc cgaccggcca gcaggggaat ccgttcgctg
     1801 ggatgcgccc cgggcagtcg gtggggggcg ctgcggcggg cgtcagccgg tgcgggtgcg
     1861 cgacagcagc tggagttcct cggcccggcg gaccgccgcc cggcggttgt tcacccccag
     1921 cttgccgaag atgttcttgg tgtgggtccg cagggtgttc agcgagatca gcagctcgtg
     1981 ggcgatggct gggccgccca ggtcggtggc gagcagccgc agcacggcgc gctcgcgctc
     2041 gctcaacggc tcgaccaagt cctgcttggc gggcgtgcgc tccctggccc cgccgaacgc
     2101 cgcgaggagg cggcgggggt agtccggcgc gatgccgcgc cgcgccgcca tttccagcaa
     2161 gtccgccatg ggcggaccct cgtccacgaa gagacggatg tagccctccg gttcggccag
     2221 cgccagggca cgccctagcg acgccagcgc ggcggggagg tcgccgcgcg tccggtgggc
     2281 gagcgcctgc aacgccaggg tctcgatgac gctccccgag cgcccccccg cttccgccgc
     2341 gtccaggaga cgcgccagga accccagggc ctcacgtacg gtgccctctg cgcggtcgcg
     2401 cgcgtggcga gccaggagca cccgggctag ggtgagatgc tcgtactcgc gcaggtagct
     2461 caggtcatcg tcggcggaca ggccccgctc gcgcgcccag tcctcggcgt cgtccagccg
     2521 cccctgcgtc agccagacgc gggccacgag cgccgcgatg gggcgcacct cggggtagaa
     2581 gtcgcccacg tagacgcgcg ccgcctcatc gagcagcgcg agcgcgcccg ctggatcgcc
     2641 ctgcgcctct tggagacgcg ccgccgcgat gcgccggcga taggggtgct gcgggaaccc
     2701 ggtgtgctcg ccttgttcct cggcgcgccg caggtgccgc gtggcagcgt ctaggtcgcc
     2761 ccattcgcgg tggagttcgg ccaggccgac gtgcatgtcg gccgtccccc gcagcgccgg
     2821 cgcgccctgc tccgccgcca gccgcaaggc cccctcgtag gtgcgcgccg cctcgcggag
     2881 ctgcccctgg gtcatccgaa tctcggccag ggcgagggtg cccccgacaa cgtcggcgct
     2941 gcttccggcc tgctggagca gcgccagccc cgcggcgaac gtccggtgcg ccgtctccag
     3001 ctcgccgcgt gcccaggcgg cgagcccgag gaagcccgcc gcccccccgc gggagagctg
     3061 gtcatcctcg gcggcgactt cgagcactcg ccgggcgtgg tgcgccg
//
LOCUS       DASUBI010000055         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1278984, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000055 DASUBI010000000
VERSION     DASUBI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278984"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="mltG"
                     /locus_tag="VFU72_00950"
     CDS             <1..438
                     /gene="mltG"
                     /locus_tag="VFU72_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5422082.1"
                     /translation="AQQLGLNFFQVMTIASIVEREAAVDSERPIIASVYYNRIKQNMP
                     LQADPTVQYAMGNAQNDWWPQLQPGDPQKINSPYNTYQSPGLPPGPICNPSLKSIQAA
                     LNPANTTFLYFVAKNDGSGEHYFANTLDEQKANIQKSEQNSSP"
     gene            435..1283
                     /gene="aroE"
                     /locus_tag="VFU72_00955"
     CDS             435..1283
                     /gene="aroE"
                     /locus_tag="VFU72_00955"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5422083.1"
                     /translation="MMTSSTLRVGLVGYPVEHSLSPVMQQAAFDALGIAARYELWPTR
                     PEELAERVAGLRHPDALGANVTVPHKTRVLPLLDEVSPLARRAGAVNTIISRAGRLHG
                     DNTDVPGFLAPLRARGLDFGQTDAVILGAGGAARAVAVALADAGCRSVLVANRTPERG
                     AALARELGGPLAAAPLDVRLTGPLSRATLLVNATAVGWTGEELPLDAALLDSLPPGGL
                     VYDLTYRQTPLLRAAEARGLATLDGFVMLVQQGVAAFRLWTGQEPPQELMLAAARSAR
                     ERHAHP"
     gene            1581..2306
                     /locus_tag="VFU72_00960"
     CDS             1581..2306
                     /locus_tag="VFU72_00960"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU5422084.1"
                     /translation="MTGVEVLQVLFGGVAGAAAGSLIHAAATRLPAGLGLLGAPRCPE
                     CGSELPTVSLLPGSSAHCSNCGWQDNVRRRATELAGALLTMLAIYGHGAAPAGLAIAA
                     FSLLLLLILRIDWQHHLIYTTTIAPGLVLALGFAALRSPADLLSATLGALLAAGVFGL
                     FFALGIVIYHRHALGAGDILLAGLIGAMTGLNHVLPAIFIGMLLAACGGLLLVLLGLR
                     SRRDYIPYGAYLCAGAMLILLLR"
     gene            2350..3000
                     /locus_tag="VFU72_00965"
     CDS             2350..3000
                     /locus_tag="VFU72_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422085.1"
                     /translation="MEPLDTRRSIPTNVTDTRHDLSPGSPPGMSLPVAASRLAAAWAL
                     LYAFYRGYYALGGTIGMFGTPVSESQWRLVNAAGAAILLVAAIAPLALLRLWPRPRLR
                     PFLFAFCWVVTVGCVMHALIDAAQRALSLAGLLTIDLPFWATVDRRKADLQDLLWNEP
                     WFLIEGLLWAAIAWNAGLKQSPRRALWLGSALAAIAALTVVGLLSALGVIGKLIVG"
     gene            complement(3080..3415)
                     /locus_tag="VFU72_00970"
     CDS             complement(3080..3415)
                     /locus_tag="VFU72_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU5422086.1"
                     /translation="MALEGGKVLRVYIGEADHWKGKPLHQAIVELLRAEGLTGATVLR
                     GVEGFGANSRLHTAHLLRLSEDLPMVVETVDRAEKIAAVLPRLKEMVRQGLITLADVE
                     LVHDGEGGG"
BASE COUNT          469 a         1293 c         1148 g          547 t
ORIGIN      
        1 gcgcagcagc tcggactgaa cttcttccag gtgatgacca tcgcctcgat cgtcgagcgc
       61 gaggccgccg tcgattccga gcggccgatc atcgcctcgg tctactacaa tcgcatcaag
      121 cagaacatgc ccctgcaagc cgacccgacg gtgcagtacg ccatgggcaa cgcgcagaac
      181 gactggtggc cgcagctcca gccgggcgac ccgcagaaga tcaactcgcc ctacaacacc
      241 tatcaatcgc ccgggctgcc gccgggaccg atctgtaacc ccagcctgaa gtcgatccag
      301 gcggcgctca acccggcgaa cacgaccttc ctctacttcg tcgccaagaa tgacggcagc
      361 ggtgagcact atttcgccaa cacgctcgac gagcagaagg ccaacatcca gaagtccgag
      421 cagaattcgt cgccatgatg acatcgagca cgctgcgtgt cggcctagtc ggctatccgg
      481 tggagcatag cctttcgccg gtgatgcagc aggctgcctt cgacgcgctg ggcatcgccg
      541 cccgctacga gctctggccc acgcgccccg aggagctggc cgagcgcgtg gccgggctgc
      601 gccacccgga cgcactgggg gccaacgtca ccgtgccgca taagacgcgg gtgctgccgc
      661 tgctcgacga ggtcagcccg ctggcgcggc gggccggagc ggtcaacacc atcatcagcc
      721 gcgcgggccg gctccacggt gacaacaccg acgtgcccgg cttcctggcc ccgctgcggg
      781 cacgcgggct cgacttcggc cagaccgacg cggtcatcct gggcgcgggc ggcgcggcgc
      841 gggccgtggc cgtggcgctg gcggacgccg gctgccggag cgtgctggtg gccaaccgca
      901 cgcccgagcg cggcgcggcc ctggcgcgcg agctgggcgg gccgctggcg gccgcgccgc
      961 tcgatgtccg gttgaccggg ccgctgagcc gggccacatt gctggtcaac gccaccgccg
     1021 tcggctggac cggcgaggaa ttgccgctcg acgccgcgct cttggacagc ctgccgcccg
     1081 gcggcctggt ctacgacctc acctaccgcc agacgccgct gctgcgggcc gccgaggccc
     1141 gcgggctcgc cacgctcgac ggcttcgtca tgctggtgca gcagggggtg gccgccttcc
     1201 gcctctggac cggccaggag ccgccgcaag aactgatgct ggccgccgcc cgatctgccc
     1261 gcgagcgcca cgcccacccg taggtcgtct aggcgctttc cgacgaacag gttgggagcc
     1321 gtgtgaggac ggatccttcg acaagctcag cgtctgccct gagcgcagtc gaaggatccg
     1381 ccctggaccg gcaccccacc tcgtcgaagg cctcggcagc ggtcccggaa gaaggcccct
     1441 acccctggca gtgctccgat tcctcagata cagcctagct gaacgccgcg agaagcagga
     1501 ccggattccc acggcccgcc gtccgcgatg ggcgtagcct tgggcgagtg gctgttgggg
     1561 ctcgtgaagc ggcggtgcgc atgaccgggg tcgaggttct gcaggtgctg ttcggcggcg
     1621 tggccggcgc ggcggccggc tcgctgattc acgcggccgc gacgcgcctg cccgccggac
     1681 tggggctgct cggcgcgccg cgctgccccg agtgcggcag cgagctgccg accgtatcgc
     1741 tgctgccggg tagctccgcg cactgctcca actgcggctg gcaggacaac gtccgccggc
     1801 gcgcgaccga gctggccggc gcgctgctga ccatgctggc gatctacggc cacggcgccg
     1861 cgccggccgg cctggccatc gccgccttca gcctgctgct cctgctgatc ctgcgcatcg
     1921 actggcagca tcatctcatc tacaccacca ccatcgcgcc ggggctggtg ctggcgctgg
     1981 gcttcgccgc gctacgctcg ccggccgatc tcctgtcggc cacgctcggc gcgctgctgg
     2041 cggcgggcgt cttcggtctc ttcttcgcgc tgggcatcgt gatctaccac cgccacgcgc
     2101 tgggggccgg cgacatcctg ctggccgggc tgatcggcgc catgaccggc ctcaaccacg
     2161 tcttgcccgc catcttcatc ggcatgctgc tggccgcgtg cggcggcctg ctcttggtgc
     2221 tgctcgggct gcgctcgcgc cgcgactaca tcccatacgg cgcctacctc tgcgccggag
     2281 cgatgctgat cttgctgctg cgctagatcc gcgctgcaaa caattgcggc gggttgttag
     2341 actcaggtaa tggaacctct cgacacccgc cgttcgattc ccactaatgt caccgacacc
     2401 cgccacgacc tgagccccgg cagcccgccc ggcatgtcgc tcccggtcgc ggccagccgg
     2461 ctcgcggcgg cctgggcatt gctctacgcg ttctatcgcg gctactacgc gctcggcggc
     2521 accatcggga tgttcggcac ccccgtctcc gaaagccaat ggcggctggt caacgccgcg
     2581 ggtgctgcca tcctgctggt ggcggcgatc gcgccgctgg ccctgctgcg actctggccc
     2641 cggccgcgcc tgcggccatt cctgttcgcg ttctgctggg tcgtcaccgt cggctgcgtg
     2701 atgcacgcgc tgatcgacgc ggcgcagcgc gcgttgagcc tggcggggct gctgacgatc
     2761 gacctcccct tctgggccac cgtcgatcgg cggaaggcgg acctgcagga tctgctctgg
     2821 aacgagccgt ggttcctgat cgaagggttg ctctgggccg ccatcgcctg gaacgccggg
     2881 ctcaagcagt cgccgcgccg cgcgctctgg ctcggcagcg cgctggccgc gatcgcggcc
     2941 ctcaccgtgg tcgggctcct cagcgcgctc ggcgtcatcg gcaagctgat cgtcgggtga
     3001 ccccgttcgc ggtgcctcag ccttggctcc gccgtgcctc cgtcgcggtc gggacagacc
     3061 cctatgcctg tgcctgccgc tacccgccgc cctccccatc atggacgagc tcgacgtcgg
     3121 ccagggtgat cagaccctgc cgcaccatct ccttgaggcg cggcagcacc gccgcgatct
     3181 tctcggcccg gtcgaccgtc tcgacgacca tgggcagatc ctccgacagg cgcagcaggt
     3241 gcgccgtgtg caggcgcgag ttggcgccga agccctcgac gccgcgcagc acggtggcgc
     3301 cggtcagccc ctcggcgcgc agcagctcca cgatcgcctg gtggagcggc ttccccttcc
     3361 agtgatcggc ctcgccgatg tagacgcgca gcactttacc gccttcgagc gccatgggtc
     3421 cgtctcctta caccagccgc gccagcacga tacccag
//
LOCUS       DASUBI010000056         4909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_926372, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000056 DASUBI010000000
VERSION     DASUBI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926372"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFU72_00975"
     CDS             complement(<1..353)
                     /locus_tag="VFU72_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5422087.1"
                     /translation="MAEARSAVSMEALVSLAKRRGIAFPGSEIYGGLANTWEFGPLGA
                     EIKRNIRNTWWEFFGHRRDDIVGIEGGVLLHPRVWEASGHVSEFFDPLIDCRNCRTRY
                     RADNLIEDRLGQVAEG"
     gene            complement(452..1444)
                     /locus_tag="VFU72_00980"
     CDS             complement(452..1444)
                     /locus_tag="VFU72_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5422088.1"
                     /translation="MTRSDVLVPRGLDGDRDRVANWGNGMQEVIVDFLETLGQEREFS
                     PNTIAAYRNDLVQFSNYLGKHYSLADWHALNDDVLRSYDLDLRERDYAKSTVARKLAA
                     IKSFCTFLVEQHGAKLHPGEALSPPRVDKFVPQAITEGEVHALLDVVDRQPGPGGLRD
                     RAMLRLLCATGLRVSELTALDLDDYLADGLVLCRRKPDRQRTVPLSPDAVDALEEYLR
                     FGRDQLTQQRTEPALFVNQRGTRLTRQGFWLILKSYAEASNLPDITPHTLRHTFAALA
                     VGAGAELHDVQHILGHVSISTTQVYQQVAQQVCGASRHHLVGSVASGYDSQSSE"
     gene            complement(1514..2626)
                     /gene="ald"
                     /locus_tag="VFU72_00985"
     CDS             complement(1514..2626)
                     /gene="ald"
                     /locus_tag="VFU72_00985"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5422089.1"
                     /translation="MLIGVPKEIKDNENRVSTTPGGVSEYVHHGHTVLVERSAGAGSG
                     FSDEEYAEAGGQLVDQHAEVFTRADMIIKVKEPLPAEYPLLRQNQILYTYLHLAAEPE
                     LTRALVRQQVTAIAYETVQLPDRSLPLLAPMSEVAGRMSIQVGAHYLQKPEGGRGVLL
                     GGVAGVPPARVVVVGGGIVGTNAAEMALGLQADTTVIDINVDRLRYLEHVLHGRFTTL
                     TSSRHNIAEAVKGADLVVGAVLIPGAKAPKLVTREMVATMRPGAVIVDVAIDQGGSVE
                     TIHPTAHSNPVYTESGVIHYGVTNMPGAVPRTSTFALSNVTLPYGLKLADLGFTAAVE
                     RDPALAKGVNVYHGHVTYHSVAEALGLPYTPLADVV"
     gene            2834..4153
                     /gene="rseP"
                     /locus_tag="VFU72_00990"
     CDS             2834..4153
                     /gene="rseP"
                     /locus_tag="VFU72_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5422090.1"
                     /translation="MAALYIIPILAALILIHEFGHFITARMAGIRVHEFGIGLPPRVA
                     GFTRNGILYSFNALPIGGFVRVLGEDGKSFAPDSLQAKSRARRALFYGAGSLMNLLLA
                     FVLITVLVGGRGDTTTHVYVAGVEQDSPAAQAGWQVGDQFLMINGKSITSVEQMVGNT
                     DNFVGEPMSVTLLRGDQTVQTTVVPRLNPPPGQGRTGIEVISAPASRISVDTVTAGSP
                     AAQAGIQPGDTLLTIAGHPIKDGQALLLELQAHQGESIPLRLERDGQPREIQVQVPRA
                     DSAGAQPSIGIGLTQHVITQPVAWWKVVPRGVAETFNSLHLMVTGVVSLVQGQATLSG
                     VAGPIGMGQLTSEMLKLSPEPAWVTLLNISALLSLNLALLNLIPFPALDGGRLFFLLI
                     EIVRGKRVPPEKEGMVHFVGLVVLLTFMLVVAYHDIDRIVSGTPFIR"
     gene            4177..>4909
                     /locus_tag="VFU72_00995"
     CDS             4177..>4909
                     /locus_tag="VFU72_00995"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5422091.1"
                     /translation="MLAPRRKTRALHVGDVQIGGGAPIVVQSMTTADTRDPKATLRQI
                     QELADAGCEIVRIAVPDRVAAAALPEIVPFAPVPLIADIHFEHTLALKALDAGIHGLR
                     LNPGNIRKPEDVREVVKKAKERQVPLRIGVNFGSVPPMSAAFVDEMAQQNATQIELIA
                     EHMVRTALGHVKILEDLDFGDIKISLKAFEVPIMIEAYRRMARLNDYPLHLGVTEAGT
                     PKAGSVRSAVGLGTLLAEGIGDTIRV"
BASE COUNT          797 a         1667 c         1592 g          853 t
ORIGIN      
        1 ccctccgcca cctggcccag ccgatcctcg atcagattgt cggcccggta gcgcgtgcgg
       61 cagttacggc agtcgatcag tggatcgaag aactccgaga cgtggccgga ggcttcccac
      121 acgcgtgggt gcaacagcac gccgccctcg atgccgacga tatcgtcgcg ccgatgaccg
      181 aagaactccc accaggtatt gcggatgttg cgcttgatct cggcgccgag cggcccgaac
      241 tcccaggtgt tcgccagccc gccgtagatc tcgctgccgg ggaaggcgat gccgcgccgc
      301 ttggcgaggc ttaccagagc ctccatcgag acggcggatc gggcctctgc catgctcagc
      361 ctacctccag agtgaccaac cccccgtctg gtggacgggg ggtgtcgcgt tccatcacgt
      421 gagttgccga gcggtaggtt cccgtcaggc gctactcact cgattgcgaa tcgtagcctg
      481 aggcgacgga cccgacgagg tggtgccggc tggcgccgca gacctgctga gcgacctgct
      541 gatagacctg cgtcgtggag atgctgacgt gcccgaggat gtgctgaacg tcgtgcaatt
      601 cggctccggc cccaacggcg agtgcggcga acgtgtgccg gagcgtatgc ggggtgatgt
      661 ccgggagatt gctggcctcg gcgtagctct tgaggatgag ccagaagccc tggcgcgtta
      721 ggcgagtgcc gcgctgattg acgaagagtg ccggctcggt gcgctgctgc gtcaactggt
      781 cacgcccgaa gcgcaggtat tcctccagcg cgtcgaccgc gtccggcgag agtggcaccg
      841 tgcgctggcg gtccggcttg cgccggcaga ggaccaaccc gtccgccaga tagtcatcca
      901 ggtcgagtgc ggtcagctcg ctgacgcgca gcccggtggc gcacagaagg cgtagcatgg
      961 cgcgatcgcg cagcccgccc ggtcccggct gccggtcgac cacgtcgagc agcgcgtgca
     1021 cctcgccctc ggtgatcgcc tgcggcacga acttgtcgac ccgcggcggc gaaagcgctt
     1081 ccccagggtg gagcttggcg ccgtgctgct cgaccaggaa ggtacagaac gatttgatcg
     1141 ccgccagctt gcgggccacg gttgatttgg cgtagtcccg ctcgcgcaga tctagatcgt
     1201 agctccggag cacgtcgtcg ttcagggcgt gccaatccgc gaggctgtag tgcttgccga
     1261 ggtagttcga aaattggacg agatcattgc ggtaggcggc gatggtgttg ggcgagaact
     1321 cgcgctcctg tcctagcgtc tccaggaagt cgacgatcac ctcttgcatg ccattccccc
     1381 aattggcgac ccgatcgcgg tccccatcca ggccgcgcgg cacgagcaca tccgacctcg
     1441 tcacgggtcg gccttgccgc tgacgggtgc gcgcccggtc ttttggcccg cgttcgtacg
     1501 agacacctcc attctaaacc acatcggcca gcggcgtata ggggagcccc agcgcttcgg
     1561 caaccgagtg gtaggtcacg tgaccgtggt agacgttcac gcccttggcc agtgcggggt
     1621 cgcgctcgac cgcggcggtg aagccgagat cggccagctt gaggccatac ggcagcgtca
     1681 cgttgctcaa cgcgaacgtg ctggtgcgcg gcaccgcgcc cggcatgttg gtgacgccgt
     1741 agtggatgac acccgattca gtatagacgg gattgctgtg cgccgtcggg tgaatcgtct
     1801 cgaccgagcc cccctggtcg atggccacgt ccacgatcac cgcgcccggc cgcatggtgg
     1861 caaccatctc gcgagtaacc agcttgggcg ccttggcgcc ggggatcagc accgcgccga
     1921 ccaccaggtc ggcgcccttg accgcttcgg cgatgttgtg gcgcgacgag gtgagcgtgg
     1981 tgaagcggcc gtgcagcacg tgctccaggt agcgcaggcg gtcgacattg atatcgatga
     2041 ccgtcgtatc ggcctgcaat ccgagcgcca tctcggcggc gttcgtcccc acgatgccgc
     2101 cgccgacgac gacgactcgc gcgggcggta cccccgcgac gccgcccagc agcaccccgc
     2161 gacccccttc cggcttctgc aagtagtgag cgccgacctg gatcgacatg cgtccggcca
     2221 cctcactcat cggcgccagc agcggcagcg agcgatccgg aagctgcacc gtctcgtagg
     2281 cgatcgccgt cacctgctgc cgcaccagcg cgcgcgtcag ctccggctcc gccgccaggt
     2341 gcagatacgt gtagagaatc tggttctggc gcagcagtgg gtactccgcg ggcagcggct
     2401 ctttgacctt gatgatcata tcggcgcgcg tgaagacctc ggcatgctga tcgaccagtt
     2461 gcccgccagc ctccgcgtac tcctcatcgc tgaaaccgct gccggcgccg gccgagcgct
     2521 cgaccagcac cgtatggccg tgatggacgt attcggagac cccacccggc gtcgtcgaca
     2581 cgcggttctc gttgtccttg atctccttgg ggaccccgat cagcatcatt cactccctct
     2641 ccaccctcgc gccgcgttgg tgcggcgcgc cgattttcga atccgaggca ttctagcacg
     2701 ggccgatcgg gcgccaagta ccagtacttt cgtcgttgac ccagggtcgt tccgcccagt
     2761 actattaggt gcgtgacagc gaaccgccgt ttgttcacta ttgcgcggag tgtcgcgcag
     2821 cgaaggtgtg accgtggcag cactctatat tatcccgatc ctcgcggctc tcatcctgat
     2881 ccacgagttc ggtcacttta ttacggcgcg catggccggc atccgcgtgc acgaattcgg
     2941 catcggtctc ccgccgcgcg tggccggctt cacgcgcaac ggcatcctct actcgttcaa
     3001 cgcgctgccg atcggcgggt tcgtgcgcgt gctcggcgag gatggcaagt cgttcgcacc
     3061 tgacagcttg caagccaagt cgcgcgcccg ccgcgccctc ttctacggcg ccggctcctt
     3121 gatgaacctc ttgctcgcct tcgtcctgat cacggtgttg gtcggggggc gcggcgacac
     3181 gaccacccac gtctatgtcg cgggggttga gcaggattcc cctgccgccc aggctggctg
     3241 gcaggtgggt gatcagtttc tgatgatcaa cggcaagtcg atcaccagcg tcgagcagat
     3301 ggtcggcaac accgataact tcgtcggcga gccgatgtcg gtcacgctgt tgcgcggcga
     3361 ccagacggtg cagacgaccg tggtgccgcg cctcaatccg ccgcccggtc agggccgcac
     3421 cggtatcgaa gtcatcagcg cgcccgcctc gcgcatctcg gtcgacacgg tgaccgcagg
     3481 ctcgcccgcc gcccaggccg gtatccaacc gggcgatacc ctgctgacca tcgccggcca
     3541 tccgatcaag gatgggcaag cgctcctcct ggagctgcaa gcgcaccagg gcgaaagcat
     3601 cccgctgcga ctggagcgcg acggccagcc gcgcgagatc caggtgcagg tgccgcgggc
     3661 agactcggcg ggcgcccaac cgtccatcgg tatcggcctg acgcagcacg tcatcaccca
     3721 gccggtggcc tggtggaagg tcgtaccgcg tggtgtggcc gaaacgttca actcgctgca
     3781 tctgatggtg acaggcgtgg tcagcctggt gcagggccag gcgacgctca gcggcgtcgc
     3841 cggaccgatc ggcatggggc aactgacctc ggagatgctc aagctcagcc ccgaaccggc
     3901 ctgggtaacg ctgctcaaca tctcggcgct gctctcgctg aacctggcgc tgttgaactt
     3961 gatccccttc ccggcgctcg acggcgggcg gctcttcttc ctgctgatcg agatcgtccg
     4021 cggcaagcgc gtgccgccgg agaaggaggg catggtccat ttcgtcggat tggtggtgct
     4081 gctaacattc atgctggtgg tcgcctacca cgacatcgat cgcatcgtaa gcggcacgcc
     4141 cttcatccgc tagcgccctg aggaggttca cgacccatgc ttgcccctcg ccgcaagacc
     4201 cgcgcgcttc acgtcggtga cgtgcagatc gggggcggcg cgccgatcgt ggtgcaatcg
     4261 atgaccacgg ccgacacccg cgacccaaag gcgacgctgc gccagatcca ggaactcgcc
     4321 gacgcgggct gcgagatcgt gcgcatcgcc gtacccgacc gcgtcgccgc cgcggcgctg
     4381 cccgagatcg tgcccttcgc gccggtgccg ttgatcgccg atatccactt cgaacacacc
     4441 ctggcgctca aagcgctgga tgccggcatc cacggcctgc gcctcaaccc cggcaacatc
     4501 cgcaagccgg aggatgtgcg cgaggtggtc aagaaggcca aggagcggca ggtgccgctc
     4561 cgtatcgggg tcaacttcgg ctccgtgccg ccgatgagcg ccgccttcgt cgacgagatg
     4621 gcgcagcaga acgcgaccca gatcgagctg atcgcggagc acatggtccg caccgcgctc
     4681 ggccacgtga aaatcctgga agacctcgac ttcggcgaca tcaagatctc gctcaaggcc
     4741 ttcgaagtgc cgatcatgat cgaggcctac cgtcgcatgg ccaggctcaa cgactacccg
     4801 ctgcatctcg gcgtgaccga agcgggcacg cccaaggccg gctcggtgcg ctccgccgtt
     4861 gggctgggca cgttgctggc cgagggcatc ggcgacacca tccgtgtct
//
LOCUS       DASUBI010000057         4198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_788855, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000057 DASUBI010000000
VERSION     DASUBI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788855"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFU72_01000"
     CDS             complement(<1..367)
                     /locus_tag="VFU72_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422092.1"
                     /translation="MRQHGLQMMASAPQERARDRRATFRRLFGELHGYNHQIVVVLLL
                     IVLSAGAQAAGPWLISRAIDRHILRHDLGGLSRLMVVLFVIYVSGSLANRAQIRRIGA
                     IGQSILAGLRQQIFDRLERL"
     gene            complement(394..2229)
                     /locus_tag="VFU72_01005"
     CDS             complement(394..2229)
                     /locus_tag="VFU72_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422093.1"
                     /translation="MQRPGNAPAHGGPRGMERVPGSGKALRRALGYLRNYTGDALGAF
                     IALLIASGANLVAPQLIRYAIDQGLGQKESRALLIAVAGLLAVALVRGGGNFLQGYLA
                     EKASQGVAYDLRNALFAKVQRLSFSYYDQMPTGQLLTRLTNDVEQIRTFAGTGVLQAG
                     SSAVMLVGTTILLLTISWQLTLIILLIIPAILALLFVFVRSISPLFIQVQQTLGLLNT
                     ILQEDLTGIRTIRAFAREEHESARYAEVNQGLFGKNMETIYAMSNNFPFVFLLANLGT
                     LAIIWFGGWQVIGGRLSIGSLIAFNSYLSFMIFPLLALGFLAAQISRAGASSVRVFEI
                     IDAPVEVQDRPGARPLPPLTGQVTFDDVHFRYPGSEREILSGVSFSVAPGQTVAILGT
                     TGSGKSTVANLIPRFYDVTGGAVLLDGYDVREVTLASLRSQIGIVLQETFLFSGTVRD
                     NIAYGHPDATRADIEAAARAAQAHDFIAALPAGYDTVVGERGVGLSGGQRQRIAIARA
                     LLVDPRLLILDDSTSSVDAETEAAIQDALDGLMRSRRHTAFVIAQRISTVRDADLILV
                     LDEGRIVARGTHQELLETSELYNEILGSQLAPDRAALAGDPTVAA"
     gene            2441..3334
                     /locus_tag="VFU72_01010"
     CDS             2441..3334
                     /locus_tag="VFU72_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422094.1"
                     /translation="MLLALIVALVLLPLAAVLLIAWSAARQALRPGPPHYDWSLADFP
                     ELPCEPVRLPGAGGVELAGRFFPGASPATIVLSHGYGDTQDQMLPWAAFLHRAGFSVF
                     SYDMRHRGQSGGRYVTLGALEQHDLAAVIDYLATRPDVDPARIGALGVSLGGSTTILA
                     AARDARIRAVVDDCGFGDAPSVIAASFERFVHLPRFPFAPLTVRLAEWRAGVSVHTVR
                     PVDVVGRLSPRPLLIIHGTADIHVPPEHGEALFAAAGEPKELWRVPEVAHAEARQDST
                     AAYERRVVDFFARALGVKPVP"
     gene            3331..>4198
                     /locus_tag="VFU72_01015"
     CDS             3331..>4198
                     /locus_tag="VFU72_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5422095.1"
                     /translation="MSGREVRAPEECRALLEAAFPGLPLGAVRFLAEGWDNTAFLVDE
                     ALVFRFPRHALGDRLLTAEITLLAAIGARLPLPTPDYRHVCGPVAGFPWHIAGYRLIP
                     GRPLRDLALGDVERLAAPLAGFLAALHATPARLLPAALPRFTPEGWLALHDRLLAEAR
                     PALGRVDAALAGRVAAWWAEYRSDPHALAFAPALIHGDLASEHILVDESGRPSGVIDF
                     GDARLADPALDLAGLPDTLFEAVARQRAHLDAGAPLRRAAYRRLAPLHAIVAARRLGR
                     PELLDEGLTGLER"
BASE COUNT          583 a         1490 c         1504 g          621 t
ORIGIN      
        1 gcaatcgctc cagccggtcg aagatctgct gccgcagccc ggccaggatg ctctggccga
       61 tggcgccgat gcgccggatc tgggcgcggt tggccagtga cccgctgacg tagatcacga
      121 acaggacgac catcagtcga ctgaggccgc cgagatcgtg ccgcaagata tgccgatcga
      181 tggcgcgact gatcagccag ggcccggccg cctgggcgcc ggcgctgagg acgatcagca
      241 gcagcaccac cacgatctgg tggttgtagc cgtgcagctc gccaaagagc cgccgaaagg
      301 tcgcacgccg gtcacgcgcc cgctcctggg gcgcgctggc catcatctgg agcccgtgct
      361 gtcgcatcgt cgttcagtcc tctttcacgc gcctcaggcc gccacggtcg ggtcgcccgc
      421 cagcgcggcg cggtccggtg ccagctgcga gccgagaatc tcgttgtaga gctcgctggt
      481 ctccagcagc tcctggtgcg tgccgcgcgc cacgatgcgc ccctcgtcga gcaccaggat
      541 cagatcggcg tcgcgcaccg tgctgatgcg ctgggcgatg acgaaggcgg tgtgccgccg
      601 gctgcgcatc agcccgtcga gcgcgtcctg gatcgccgcc tcggtctcgg cgtcgaccga
      661 cgaggtgctg tcgtccagga tcaagaggcg cggatcgacc agcagcgcgc gggcgatggc
      721 gatgcgctgg cgctgcccgc ccgagagccc caccccacgc tcgccgacga cggtgtcgta
      781 gcccgcgggc agcgcggcga tgaagtcgtg cgcctgtgcg gcgcgggccg cggcctcgat
      841 atccgcccgg gtggcgtcgg ggtggccgta ggcgatgttg tcgcgcacgg tgccgctgaa
      901 gaggaaggtc tcctgcaaga cgatgccgat ctggctgcgc aggctggcga gcgtcacctc
      961 gcgcacgtcg tagccgtcga gcagcaccgc gccgccggtg acgtcgtaga agcgcgggat
     1021 caagttggcc accgtgctct tacccgagcc ggtggtaccg agaatcgcga ccgtctgccc
     1081 cggcgcgacg ctgaagctga caccgctcag gatctcgcgc tcgctgccgg gatagcggaa
     1141 gtggacgtcg tcgaacgtca cctggccggt gagcggcggc agcggccggg cgccggggcg
     1201 atcctgcacc tcgaccggcg cgtcgatgat ctcgaaaaca cgcaccgacg aggcgccggc
     1261 gcgcgagatc tgcgccgcca ggaagccgag cgccagcagt gggaagatca tgaagctgag
     1321 gtagctattg aacgcgatca gcgagccgat cgagagccgg ccgccgatga cctgccagcc
     1381 gccgaaccag atgatcgcca gcgtgcccag gttggccagc aggaagacga aggggaagtt
     1441 gttcgacatg gcatagatcg tctccatgtt cttgccgaac aagccctggt tgacctcggc
     1501 gtagcgcgct gactcgtgct cttcacgggc gaacgcgcgg atggtacgaa tgccggtcag
     1561 gtcctcctgc aggatggtgt tgagcagccc cagggtctgc tgcacctgga tgaagagcgg
     1621 gctgatgctg cgcacgaaga cgaacaggag cgccaggatg gccgggatga tcagcaggat
     1681 gatcagggtc agttgccagc tgatggtcaa gagcaggatg gtggtgccga ccagcatgac
     1741 cgccgacgag ccggcctgca gcacgccggt gccggcgaag gtgcggatct gctcgacgtc
     1801 gttggtcagc cgggtcaaga gctgaccggt cggcatctgg tcgtagtagc tgaagctgag
     1861 ccgctggacc ttggcgaaga gggcgttgcg cagatcgtag gccaccccct gcgaggcttt
     1921 ctcggccaga tagccctgca ggaagttgcc gccgccgcgc accagcgcca ccgccagcaa
     1981 gccggcgacc gcgatcagca gcgcccgcga ctccttctgg cccaggccct ggtcgatggc
     2041 gtagcggatc aactgcggcg ccaccaggtt ggcgccggag gcgatcagga gcgcgatgaa
     2101 cgcgccgagc gcgtcgccgg tatagttgcg caggtagccg agcgcccggc gcaacgcctt
     2161 gccgctcccc ggcacccgct ccatgccgcg cggcccgcca tgagccgggg cgttacccgg
     2221 tcgctgcatt ggccatcctc cagaaagggt tcgacgtcat cgaagcactc tattatgcca
     2281 cgccgggaaa ggcagcggcc acggtttgcc ggcgtgtggc ggccgccgac tactcggcgc
     2341 gacgcgtgga gacggatcct ttccaaactc ccccggcagc cgcctcccca ctcccgcacg
     2401 aatgggaaag ggagcgtaac tatacggcgg caggcgagcc atgctgctgg ccctgatcgt
     2461 ggcgctcgtc ctcctgccgc tggcagccgt gctgctgatc gcctggtcgg cggcgcggca
     2521 ggcgctgagg cctggcccgc cgcactacga ctggtcgctg gccgacttcc ccgagctgcc
     2581 ctgcgagccg gtgcggctgc cgggcgcggg cggcgtcgag ctggccgggc gcttctttcc
     2641 gggcgcgtcc ccggcgacga tcgtgctcag ccacggctac ggcgacaccc aggatcagat
     2701 gctcccctgg gccgccttcc tccaccgcgc cggcttcagc gtcttcagct acgacatgcg
     2761 ccaccgtggc cagagcggcg ggcgctatgt cacgctcggc gcgctggagc agcacgacct
     2821 ggcggcggtg atcgactacc tggccacgcg ccccgatgtc gatcccgcgc gcatcggcgc
     2881 cctcggcgtc tcgctgggcg gctcgaccac gatcctggcg gcggcgcgcg acgcgcgcat
     2941 ccgggccgtg gtcgatgact gcggcttcgg cgacgccccc tcggtcatcg ccgccagctt
     3001 cgagcgcttc gtccacctgc cgcgcttccc cttcgccccg ctcacggtgc ggctggctga
     3061 gtggcgcgcg ggtgttagcg tgcacacggt ccgcccggtc gacgtggtgg gccggctcag
     3121 cccgcgcccg ctcctgatca tccacggcac cgccgacatc cacgtgccgc cggagcacgg
     3181 cgaggcgctc ttcgccgcgg cgggcgagcc caaggaactg tggcgggtgc cggaggtggc
     3241 ccacgccgag gcgcgccagg acagcaccgc cgcctacgag cggcgcgtgg tcgacttctt
     3301 cgcccgtgcg ctcggcgtga aaccggtgcc gtgagcgggc gcgaggtgcg cgcgccggag
     3361 gagtgccgcg cgctgctgga ggcggctttc cccggcctgc cgctcggcgc ggtgcgcttc
     3421 ctggccgagg gctgggacaa caccgccttc ctggtcgacg aggcgctggt cttccgcttc
     3481 ccccgccacg cgctcggcga ccgcctgctg accgcggaga tcacgctgct ggcggcgatc
     3541 ggcgcgcgcc tgccgctgcc tacgccggac tatcgccacg tctgcggtcc ggtggccggc
     3601 ttcccctggc acatcgccgg ctaccggctg atccccggca ggccgctgcg cgatctcgcg
     3661 ttgggtgatg tcgagcggct ggcggcgccg ctggccggtt tcctggccgc gctgcacgcc
     3721 accccggcgc ggctgctgcc ggcggcgctg ccacgcttca cgccggaggg ctggctggcc
     3781 ttgcacgacc gtctgctggc ggaggcgcgg ccggcgctcg ggcgggttga cgcggccttg
     3841 gccgggcgcg tggccgcctg gtgggcggag tatcggagcg atccgcatgc tctcgccttc
     3901 gccccggccc tgatccacgg cgacctcgcg agcgagcaca tcctggtgga cgagtcgggc
     3961 aggccgagcg gcgtcatcga cttcggcgac gcgcggctgg ccgatccggc gctcgacctg
     4021 gcgggcctgc ccgacacgct ctttgaggcg gtcgcgcgcc aacgagcaca cctcgacgcg
     4081 ggcgcgcccc tgcgccgcgc cgcctaccgc cgcctggcgc cgctgcacgc catcgtcgcc
     4141 gcccggcgcc tgggccggcc ggagctcttg gacgaagggc tgacagggct ggagcgcg
//
LOCUS       DASUBI010000058        10305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1280341, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000058 DASUBI010000000
VERSION     DASUBI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280341"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1530)
                     /locus_tag="VFU72_01020"
     CDS             complement(136..1530)
                     /locus_tag="VFU72_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5422096.1"
                     /translation="MERILIADDDAAIRGLLREFLEDEGYEISEAEDSSQVLAAMDSA
                     EAPQLVLMDVRMPGKSGIETLRELSEQHGTQLPFIIMTAYGSADLAIQAMQLGAYDYL
                     TKPFDLDDVLITITRYFEHQRLLKTVSDLRSGRNEVDANAYIVGNSGAMQEVFKTIGR
                     VARSDATVLVTGETGTGKELVATILHKNSGYSGGPLIKVNCAALPETLLESELFGHEK
                     GSFTGALAQHKGRFEAAHRGTIFLDEVGEMTLATQKKLLRVLQEREFERVGGSVSVKV
                     DTRVVAATNKNLIQEVAERRFREDLYYRLNVIAIFLPPLRDRKEDIPLLVEHFLHKHR
                     YTPGSSPARISSSAVELLLDHEWPGNVRELENIIERAVVLSQGTIITDEHISFSSADQ
                     RHFIDIAERVRSGIPVQRVQQEVEKQMLLEALKQTDGDRVAASVLIGVDLKDFQQRLV
                     DFGLPTDQRSPVPY"
     gene            complement(1814..3532)
                     /locus_tag="VFU72_01025"
     CDS             complement(1814..3532)
                     /locus_tag="VFU72_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422097.1"
                     /translation="MLDESSHQIAQVVSPDLFRLLVESVMDYAIFLLDPDGNVRSWNA
                     GARRIKGYTADEIIGKHFSIFYPPSEVRRGKPTYGLGVAREAGRWEEEGWRVRKDGSR
                     FWASVIITALRDPGGTLVGFAKVTRDLTERRQAEEERRQLLTLERAARAEAESSLVQL
                     QSIQRVTDAALGHLGLDELLITLLDRIGEVLEVDTVAILLLDAEDGVLVARAAKGIEE
                     EVERGVRIPLGAGFAGRIVSESRPIVLYDVPHAAVLNPILRENGVRSLMGVPLRVEQR
                     VIGVLHVGTLFPRRFTDAEQQLLQIVGDRVALAIDHAQLYEAAQAARQEADISVERLQ
                     TQDEFLSIAAHELKTPMTGLKAAVQLLLRRAERSEASLLTELARPLQTINEQTEKLAR
                     LTEQLLETARVQADLLPISRQPTDVSALVQSVVARFQEQTTQHHFQVSGPAELWANVD
                     PLRLEQVLSNLLENAIKYSGTAEPIAVELCQPHPRRIQLSVRDYGVGVPPEHRAHLFE
                     RFYQVRAGGAQGGLGLGLYISRRIVEQHGGMITADFPPGAGSRFVVALPTDADDDAID
                     AEPTGS"
     gene            3690..5369
                     /gene="aroB"
                     /locus_tag="VFU72_01030"
     CDS             3690..5369
                     /gene="aroB"
                     /locus_tag="VFU72_01030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5422098.1"
                     /translation="MVERVVLIGLSGTGKSSVGERLAAVLGFEYVDSDAEVARRFGLS
                     IADIFARYGEPVFRAAERDALRAAAERRGLVIATGGGAVLDPANWGALRPGSLIVHLT
                     ASPEQILRRLNSQIVEQPRERRPLLEQDDPEAALRRLWSARAGLYRQADVEIDTDAIS
                     IEDVAEQIVRLAADYAGDGLVPVTSIDFASGRSDIYVAPGLLVRLGVLSRRRWPEARR
                     AWIISDDHVTPLWGEVAAGALRQSGFAVHAHAVPAGESSKSMGKLEELLDWLLAGKLD
                     RRDIVVALGGGVVGDLAGFAAAIALRGVGLVQVPTSLLAMVDSSVGGKTAVNHRLGKN
                     LIGAFYQPTLVLADTATLETLPPRESRAGWAEIVKHAMIERTATGAAEAALLDLLERA
                     DERRLADAEFIAEIVRRNVLIKASVVRQDEREDGLRRILNYGHTLGHAMEAAGFRYQH
                     GEAVALGMRAAARLSLELGRTAPEIVARQNAVLDRLGLPARFEGNLAEVLERMTHDKK
                     AVAGSLTWVLLARAAGQVELSRDVPLGLVEDVATGLGAARVTTGPMGRAML"
     regulatory      5392..5499
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5607..6881
                     /locus_tag="VFU72_01035"
     CDS             5607..6881
                     /locus_tag="VFU72_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5422099.1"
                     /translation="MTGSTEARYDVKNIELAPEGRRRIDWAQREMPVLTLIRQRFEQE
                     KPLNGIRMTVCAHITTETANLAYTLRAGGAEAALCASNPLSTQDDVAAALAADGFRVY
                     AGKGEDEETYYRHIQQALEIRPNLVIDDGADVMTIIHRDRRDLLPEVIGGSEETTTGV
                     IRLRALEREGALAFPVIAVNDAQTKHFFDNRYGSGQSSLDGIIRATNVLIAGKTVVVA
                     GFGWVGRGIATRARGMGAHVIVTEVDAVKALEAVMDGFQVMPMVEAAAHGDIFITATG
                     DINVIDRPAFERMKPGAILANAGHFNSEINLHMLDEMAAEGRRDLRPFVEEYRLTPAK
                     GIIVLGEGRLINLAAAEGHPASVMDMSFANQALASAFLVRERGQLANQVYAVPPDIDE
                     EIAQLKLTSMGIQIDTLTEEQRRYLSSWQSGT"
     gene            6936..8171
                     /gene="metK"
                     /locus_tag="VFU72_01040"
     CDS             6936..8171
                     /gene="metK"
                     /locus_tag="VFU72_01040"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5422100.1"
                     /translation="MSSDFMRAAHSLFTSESVTEGHPDKLCDQISDAVLDAILAKDPY
                     ARVACESATTTGLVLVFGEITTNTYIDIPAVTRATVAEAGYTHADLGFDAETSSVLTA
                     IKEQSPEISMGVSRALEARDGEAVDELELIGAGDQGMMIGFACDETPWLMPLPISLAH
                     AVSKRLADARKSRAIPYLRPDGKCQFTVEYEHGQPLRVDTIVISTQHDPDIPKARMRD
                     DLIEGVIRPMVPEELLDDKTRILINPSGSFVLGGPMGDAGLTGRKIIVDTYGGMARHG
                     GGAFSGKDPTKVDRSGAYAARYVAKNVVAAGLADRFELQISYAIGVAHPVSLYIETFG
                     TAHVPEEKIDELIRRHFDLRPAAIIRDLDMRQPIYKKVAVYGHFGRPDLDLPWERTDK
                     AAALRDGAGLPQVEEPAIR"
     gene            8184..9122
                     /locus_tag="VFU72_01045"
     CDS             8184..9122
                     /locus_tag="VFU72_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5422101.1"
                     /translation="MRALITGASGFVGRHLGAHLLALGYEVVGLSAHHARLPDGVLAL
                     TADLLDADLTARTLLAQQPDVIFHLAAQAYVPQAIAEPGRTLANNILAQLNVLEAARR
                     LDPAPMILVAGSSEVYGVVPPEQQPVKEEQPFRPGNAYAVSKVTQDMLGLQYYLSYGL
                     PVVRARAFNHIGPGQSDRFAVASFARQIAEAEAGRSQPLLLVGNLETGRDFLDVRDVV
                     RAYVLMARHEFAGEVFNVASGVAHSVGELLDALLADATIPIEVRQDPARMRPSEAPVI
                     VGDASRLTAATNWRPRIPLGRSLRDTLDEWRARIGG"
     gene            complement(9336..10235)
                     /locus_tag="VFU72_01050"
     CDS             complement(9336..10235)
                     /locus_tag="VFU72_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEU5422102.1"
                     /translation="MLSILRRVVYFGRPCDLSAAPLAALAEAGYQVAAVVTPVRSPGQ
                     QRPLRIRPAPRLLPGSPSDALTAVASAHHIPLWEAANLAAPEVRQALAEVAPDILAVS
                     CFPWRIPRSLLALAPHGGINLHPSLLPRHRGPEPLFWVFRAGERHTGVTIHRLAPRLD
                     AGGIVAQERLAVPAGIPGDELERQAAALGARILPLALERVFSGCAAPPQDETLASYET
                     WPGEHDLALPRDWTSEHAWRFTRGVMPLGYQPTIEVDGATLTVIEALRVTCENQQHDD
                     VLQGSLLDASFAGGRLLLRVASK"
BASE COUNT         1723 a         3283 c         3564 g         1735 t
ORIGIN      
        1 ggcggtgccg cgaaagagat ccggctccgt tggctccgcc atgcgctgcc tctctcgcgt
       61 cgctcagtca ggctcggacc gacgcgcatc gcccaggcgc ggggccactc gtcctggtcg
      121 ttgagatgcc gtctgctagt acggtacggg tgagcgctgg tcggtcggca ggccgaagtc
      181 gaccagccgc tgctgaaagt ccttcaagtc gacgccgatc aggaccgagg cggctacacg
      241 atcgccgtcg gtctgcttca gtgcctcaag cagcatctgc ttctcgacct cctgctgcac
      301 ccgctgcacc gggatcccgc tgcgcacgcg ctccgcgatg tcgatgaaat ggcgctgatc
      361 ggccgaggaa aagctgatgt gctcgtcggt gatgatcgtg ccctgcgaca ggacgaccgc
      421 acgctcgatg atgttctcca gctcgcgtac gttgccgggc cattcatggt cgaggagcaa
      481 ctcgaccgcc gacgatgaga tgcgtgccgg gcttgagccg ggcgtgtagc ggtgcttgtg
      541 caggaagtgc tcgaccagca gcgggatatc ctctttgcgg tcgcgcagcg gcggcaggaa
      601 gatcgcgatg acgttgagcc ggtagtagag atcctcgcgg aagcgccgct cggcgacctc
      661 ctggatcagg ttcttgttgg tggcggccac cacgcgcgta tcgaccttga ccgacacgct
      721 gccgcccacc cgctcgaact ctcgctcctg tagcactcgc agcagcttct tctgggtcgc
      781 gagcgtcatc tcgccgacct cgtcgaggaa gatcgtgccc ctgtgcgcgg cctcgaagcg
      841 gcctttgtgc tgggcaagtg cgccagtgaa tgagcccttt tcgtggccga agagttcact
      901 ctccaggagc gtctccggca gcgcggcgca gttgactttg atcagtggtc caccggagta
      961 gccggagttc ttatgcagga tggtggcgac cagctccttg ccggtaccgg tctcgccggt
     1021 caccagcacc gtcgcgtcgg agcgggcgac gcgcccgatc gtcttaaaga cttcctgcat
     1081 cgcgccgctg ttgccgacga tataggcgtt ggcgtcgacc tcgttgcgac cgctgcgcag
     1141 atcggagacg gtcttgagga gccgctggtg ttcgaaatag cgcgtgatcg taatcaggac
     1201 atcgtcgaga tcaaacggct tggtgaggta gtcgtaggcg ccgagctgca tggcttgaat
     1261 ggcgagatca gcgctaccgt aggcggtcat gatgatgaag gggagctgag tgccgtgctg
     1321 ctccgagagt tctcgcagcg tctcgatgcc gctcttgccc ggcatacgca cgtccatcag
     1381 gaccaattgg ggcgcctcgg cgctgtccat cgcagcgagc acctgactgc tgtcttcggc
     1441 ctcgctgatc tcgtatccct catcttcgag gaattcgcgc agcagacctc ggatagcggc
     1501 gtcgtcatct gcgattagga ttcgctccat cgactctctt cccatgggtg atctcaaggt
     1561 ggctgttggg ctgcctcgtg aatccgtggc ggatcgtttg actgccagta tagcaaaccg
     1621 agtgtcctct agacacttgg aagcgacgtt caaatttagg cgcggcctcg tcgtgatgat
     1681 cgaccgggcg cccaccgcga tcctagttcg ccagtagctc tcacccgact gaccggttgg
     1741 tatggtggat acgcgagtcc gcctgtccaa accttgcgca ggtttcttgc cgcagacgtg
     1801 ggaggattcg aatctagctg ccggttggct cagcgtcgat ggcgtcgtcg tcggcgtcgg
     1861 tcggcagcgc cacgacaaag cgtgagccgg ctccgggcgg aaagtcagcg gtgatcatgc
     1921 cgccgtgctg ctcgacgatg cgccggctga tgtagaggcc gagacccagg cctccctgag
     1981 ctcccccggc gcgtacctgg tagaagcgct cgaacaagtg cgcgcgatgc tcgggcggca
     2041 cacccacccc gtagtcgcgc accgaaagct ggatgcgccg cgggtgcggc tggcagagct
     2101 cgaccgcgat cggctccgcg gtgccgctgt atttgatcgc gttttcgagc aagttggaca
     2161 ggacttgctc cagacgcaac ggatcgacgt tcgcccacag ctctgcaggg ccgctgactt
     2221 gaaagtggtg ttgagtggtt tgctcctgga agcgagcgac gactgactgc accagcgcgg
     2281 acacgtcggt aggttgccgt gagatgggca gcaggtcagc ctggactcgt gcggtctcca
     2341 acaattgctc ggtcagccgg gccagtttct cagtctgctc gttgatcgtc tgaagcggtc
     2401 gcgccagctc cgtcaacagg ctggcttccg agcgctccgc gcggcgcagc aggagttgca
     2461 cggccgcctt caagcccgtc atcggcgtct tcagctcatg cgccgcgatc gacaggaact
     2521 catcttgtgt ctggagccgc tcaaccgaga tgtcggcttc ctggcgggcc gcctgcgccg
     2581 cctcgtagag ttgcgcgtga tcgatcgcca gcgcgacccg atcgcccaca atctgcagca
     2641 attgctgctc ggcatcggtg aagcgacgag ggaagagcgt gccgacgtgc agcaccccga
     2701 tgacgcgctg ttcgacgcgc agcggcacgc ccatcaggga gcggacgccg ttctcgcgca
     2761 gaatcgggtt gagcaccgcc gcgtgcggca catcgtagag aacaatcggc cggctctcgc
     2821 tgacgatccg tcccgcgaag ccggctccca gcggaatgcg cacgcctcgc tcgacttcct
     2881 cctcgatccc cttggcggcc cgcgcgacca gcacaccgtc ctccgcatcg agcagcagga
     2941 tggcgacggt gtcgacctcg agcacctcgc cgatgcgatc cagcagggtg atgagaagct
     3001 cgtcgaggcc caggtggccc aacgcggcgt cggtgacgcg ctggatgctc tgcaactgca
     3061 ccagtgagga ttcggcctcc gcccgcgcgg cgcgctccag tgtcagaagc tggcggcgct
     3121 cctcttcggc ctggcgccgc tccgtcagat cgcgcgtcac cttcgcgaag ccgaccagcg
     3181 tcccgccggg atcccgaagt gcggtgataa tgacgctggc ccaaaaccgc gatccgtcct
     3241 tgcgtacccg ccagccctct tcctcccagc gccccgcctc gcgagcgacg cctagtccat
     3301 aggtcggttt gccgcgccgc acctcggacg gtggatagaa gatcgagaag tgcttgccga
     3361 tgatctcgtc cgcggtgtac cccttgattc gcctcgcgcc ggcgttccag ctccgcacgt
     3421 tgccatctgg atcgagcaag aagatggcgt agtccatcac actctcgacc agcaggcgaa
     3481 agagatcggg tgagacgacc tgtgcgatct ggtggctcga ttcgtcgagc attcccgttt
     3541 ctccgatccg gctgaaggag cgtgggggcc cctcgccgca ggctctgtga caagaatacc
     3601 gcagagtttg acaatgtgcc gggatcgcga caggccggcg cggagctata atcggccggt
     3661 cgcggagggc gagggaggtt gagctggcga tggtggaacg cgtcgtcctg atcggtttga
     3721 gtgggaccgg taagagcagc gtcggagaac gcctggcggc cgtgctgggt ttcgagtacg
     3781 tggacagcga cgcggaggtc gcgcgacgct tcgggctgtc gatcgccgac atcttcgcgc
     3841 gctatggcga gccggtgttt cgagcagccg agcgtgacgc gctgcgcgcg gccgcggaac
     3901 ggcgcgggct cgtgatcgcg accgggggcg gcgcggtgct cgacccggcc aactggggcg
     3961 cgctccggcc cggcagcctc atcgtccatc tgacggcatc ccccgagcag atcttgcgcc
     4021 gactgaacag ccaaatagtg gaacagccgc gagagcgacg gccgttgcta gagcaggatg
     4081 atcctgaggc cgcactgagg cggctgtggt cggcgcgcgc ggggctgtac cgacaggccg
     4141 atgttgagat cgacaccgat gcgatctcga tcgaggacgt ggccgagcag atcgtgaggc
     4201 tggcagcgga ctacgccggc gacggattgg tcccggtgac gtcgatcgac ttcgccagcg
     4261 gtcgctcgga catctacgtg gcgccggggc tgctggtgcg cctgggcgtg ctgtcgcgac
     4321 gccgctggcc cgaggcacgc cgcgcctgga tcatcagcga tgaccacgtc acgcccctct
     4381 ggggtgaggt cgcggctggc gctttgcgcc agtccggctt cgcggtgcac gcgcacgccg
     4441 tcccagcggg tgagagcagc aagtcgatgg ggaagctgga ggaactgctc gactggctgc
     4501 tggccggcaa gctcgaccgt cgcgacatcg tcgtggcgct gggtggcggg gtagtcggcg
     4561 acctggcggg cttcgcggcg gcgatcgcct tgcgcggggt ggggctggtg caggtgccga
     4621 ccagtctgct cgcgatggtc gacagcagcg tgggcgggaa gacggcggtc aaccaccggc
     4681 tgggcaagaa cctgatcggc gccttctacc agccgacgct ggtgctggcc gacactgcca
     4741 cgctcgagac gctgccgccc cgtgagtcgc gcgccggctg ggccgagatc gtgaagcacg
     4801 ccatgatcga gcgcacggcg accggagccg ctgaggcggc gctgctggat ctgttggagc
     4861 gagccgatga gcggcgtctg gccgacgcgg agttcatcgc cgagatcgtg cggcggaacg
     4921 tcctgatcaa agcgtcggtg gtgcggcagg acgagcgcga ggacggtttg cgtcgcatcc
     4981 tgaactacgg ccacacgcta gggcacgcca tggaggcggc gggcttccgc taccaacacg
     5041 gcgaggcggt cgcgctgggg atgcgcgccg ccgcgcggct cagcctggag cttgggcgta
     5101 ccgcaccgga gatcgtcgcg cggcagaacg cggtgctcga tcgcctgggt ttgccggctc
     5161 gcttcgaagg caatctagcc gaggtgttgg agcgaatgac acacgacaag aaggccgtcg
     5221 ccggcagttt gacctgggtc ctcctggcgc gcgcggcggg gcaggtcgaa ttgtcacgcg
     5281 acgtgccgct gggtctggtc gaggacgtgg cgaccgggct cggcgcggcg cgcgtcacca
     5341 ctggcccgat gggaagggct atgttatagt cggacaaaat caagacgctc tctcttatcc
     5401 cgagtggcgg aggggacggc ccgaggaagc ccggcaaccg gcgatgcgaa tcgcgaggtg
     5461 ccaattccgg cagcgactgg tgtcgctgaa agatgagagt gagcaggagt cagacggctc
     5521 tcgctcacga gcggctgtct ttttttgtgt gttttgcatg gtggtgaagc ggcagggcgt
     5581 gcgagcagcc cagagggagg cgaagagtga ccgggagcac ggaggcgagg tacgacgtca
     5641 agaatatcga gctcgcgccg gaggggcgcc ggcgcatcga ctgggcgcag cgcgagatgc
     5701 cggtgctgac gctgatccgc cagcgcttcg agcaggagaa gccgctgaac ggcattcgca
     5761 tgacggtctg cgcgcacatc accactgaga ccgctaatct cgcctatacc cttcgtgccg
     5821 gcggcgccga ggccgcgctc tgcgccagca acccgctctc gacgcaggac gacgtggccg
     5881 ccgcactggc cgcggacggc ttccgcgtct acgccggcaa aggtgaggac gaggagacct
     5941 attaccggca catccaacag gcacttgaga ttcgcccgaa cctggtcatc gacgacggcg
     6001 cggacgtgat gaccatcatc catcgcgacc gccgcgatct cctgcccgag gtcatcgggg
     6061 gctctgaaga gaccacgacc ggggttatcc ggctgcgcgc gctggagcgc gagggcgcgt
     6121 tggccttccc ggtgatcgcg gtcaacgacg cccagaccaa gcacttcttc gataaccgct
     6181 atggctccgg tcagagctcg ctcgacggca tcattcgcgc caccaacgtc ctgatcgcgg
     6241 ggaagacggt ggtagtcgcc ggcttcggct gggtcgggcg tggcatcgcc acccgcgccc
     6301 ggggcatggg agcgcatgtg atcgtgaccg aggtcgacgc ggtcaaggcg ctggaggccg
     6361 tgatggacgg cttccaggtc atgcccatgg tggaggccgc cgcccacggc gatatcttca
     6421 tcaccgccac cggtgacatc aacgtgatcg atcggccggc gttcgagcgt atgaagccgg
     6481 gcgcgatcct ggccaacgcc gggcacttta actccgagat caacctgcac atgctggatg
     6541 agatggcggc cgagggtcgg cgcgacctgc gccccttcgt cgaggagtac cgcctgactc
     6601 cggctaaggg gatcatcgtg ctgggtgagg gccggttgat caatctggcc gcggccgagg
     6661 gccacccggc cagcgtgatg gatatgagct tcgccaacca ggcgctcgca tccgcgtttc
     6721 tggtgcgcga gcgcgggcaa ctcgccaacc aggtctacgc ggtgccgccc gacatcgacg
     6781 aagagatcgc gcagctcaag ctcaccagta tgggaatcca gatcgacacc ttgaccgagg
     6841 agcagcgccg ctatctcagc tcgtggcagt ccggcaccta ggcgtggccg tcggacgaat
     6901 cgcgtgtgac tgagacgaca gggagaggaa gagacgtgag cagcgatttc atgcgggccg
     6961 cgcattcgct ctttacctcg gagtccgtga ccgaggggca tccggacaaa ctctgcgatc
     7021 agatctccga cgctgtactc gacgcgatcc tggccaagga cccgtacgcg cgcgtcgcct
     7081 gcgagtcggc gaccaccacc gggttggtgc tggtcttcgg cgagatcacc acgaatacct
     7141 atatcgacat cccggctgtg acgcgcgcca cggtggccga ggcgggctac acacacgccg
     7201 acctcggctt cgacgccgag acctcgtcgg tcttgacggc gatcaaggag cagtcgccgg
     7261 agatctcgat gggggtcagc cgcgcgctgg aggcgcgcga cggagaggcg gtggatgagc
     7321 tggagctgat cggcgccggc gaccagggca tgatgatcgg ttttgcctgc gacgagacgc
     7381 cctggctgat gccgctgccg atctcgctgg cacacgccgt cagcaagcgc ctggcggacg
     7441 cgcgcaagtc gcgggcgatc ccctatctgc ggcccgacgg caaatgtcag ttcacggtgg
     7501 agtacgagca cggccagccg ctgcgtgtcg acaccatcgt catctcgacc cagcacgacc
     7561 cggatattcc aaaggcgcgc atgcgcgacg acctgatcga aggcgtcatt cggccgatgg
     7621 tcccggagga gctgctggac gacaagacgc gcatcctgat caaccccagc ggcagctttg
     7681 tgctcggcgg gccgatgggt gatgccgggc tgaccggccg caagatcatc gtcgatacct
     7741 acggcggcat ggctcggcac ggcggcggtg ccttctcggg taaagatccg acaaaggtcg
     7801 accgctcggg cgcgtatgcg gcgcgctacg tggccaagaa cgtggtcgcg gcgggcctgg
     7861 ccgatcgctt cgagctccag atctcctacg caatcggtgt ggcacacccg gtctcgctct
     7921 acatcgagac cttcggcacg gcccacgtgc ccgaggagaa gattgacgaa ttgatccggc
     7981 gccatttcga tctgcgcccg gcggccatta tccgcgatct cgacatgcgg caaccgatct
     8041 acaagaaggt cgcggtctac ggccactttg gacgacccga cctcgacctg ccctgggagc
     8101 gcaccgacaa ggccgccgcg ctgcgcgacg gggccgggct gccgcaagtg gaggagccgg
     8161 ccattcgctg acgagaggtc tccatgcgag cgctgatcac cggcgccagc ggcttcgtcg
     8221 gccggcatct gggcgcgcat ctgctggcac tgggatatga ggtggtgggt ctttcggccc
     8281 accacgcccg tctgcccgat ggtgttctgg cgctgacggc ggacctgctc gacgccgatc
     8341 tgacggcgcg caccctactc gcgcagcaac ccgacgtcat ctttcacctg gcggcgcagg
     8401 cctacgtacc gcaggcgatc gccgagcccg gccgcacgtt ggccaacaac atcctggcgc
     8461 agctcaacgt gctggaggcc gcgcgccggc tggatccggc tccgatgatc ctggtagccg
     8521 gctcgtccga ggtctacggc gtcgtgccgc ctgagcagca gccggtcaag gaagagcagc
     8581 cgtttcggcc aggcaacgcc tacgcggtga gcaaagtgac gcaggatatg ctcggactgc
     8641 agtactacct gagctacggg ttgccggtgg tgcgggcgcg ggccttcaat cacatcgggc
     8701 cgggccagag cgaccgcttc gcggtggcga gcttcgcgcg ccagatcgcg gaggccgaag
     8761 cggggcgcag ccaaccgctc cttctggtcg gcaacctgga gacgggccgc gactttctgg
     8821 acgtgcgcga cgtcgtgcga gcgtatgtgc tgatggcgcg acatgagttt gccggcgaag
     8881 tcttcaacgt cgccagcggc gtggcgcact cggtcgggga gctactcgac gccttactgg
     8941 ccgacgctac gatcccaatc gaggtgcggc aggatcccgc gcgcatgcga cccagcgagg
     9001 cgccagtgat cgtgggtgac gcctcccggc tgacggcagc caccaattgg cggccgcgga
     9061 tcccgctggg ccgatcgctg cgcgacacgc tcgacgaatg gcgcgcacga attggcgggt
     9121 gagtgttgct gccaaaaacg ctcgtggccc gtggggcacc gatccctcca cttcggtcgg
     9181 gatgacaggg ttagggccgg actgccgagg aggaccggtg ccgggcgcga cggcacgtcg
     9241 cctggacggc tccatcccct ccccatgtca tcctgagctt gtcgaaggat ccgtcctcat
     9301 agggcaggcc cgttgtcgac agcagacttt gagcgctatt tcgacgcgac acgcagcaac
     9361 aagcgcccgc cggcaaagga ggcgtcgagc agactcccct gcaacacgtc atcgtgttgt
     9421 tggttctcgc aagtcacgcg cagcgcttcg atgaccgtca acgtcgcacc atcgacctcg
     9481 atcgtgggct ggtaaccgag gggcatcacg ccgcgggtga agcgccaggc gtgctcgctg
     9541 gtccagtcgc gcggcagagc aaggtcgtgc tcgcccggcc aggtttcgta gctggccaga
     9601 gtctcgtctt gtggtggcgc ggcgcagccg ctgaacaccc gctccagcgc gagggggaga
     9661 atgcgagctc cgagcgccgc cgcctgccgc tccagttcat cgccggggat gcccgctggc
     9721 accgccaagc gctcctgtgc gacgatgcct ccggcatcga gccgcggcgc tagccggtga
     9781 atggtgacac ccgtgtggcg ctcgccggcg cgaaagaccc agaacagcgg ctcgggcccg
     9841 cgatggcgcg gcaggagcga gggatgcaga ttgatgccgc cgtgcggggc cagggcgagc
     9901 agcgagcgcg ggatacgcca ggggaaacag gagacggcga ggatgtcagg agcaacctct
     9961 gcgagcgctt gccggacctc gggggcggcc aggttggcgg cttcccagag cggaatgtga
    10021 tgtgcgctgg ctacggcggt gagtgcgtcg gaggggctgc ccggcagcag ccgcggcgcg
    10081 ggacgaattc tcagcgggcg ctgctggccc ggcgaccgga cgggcgtgac taccgcggcg
    10141 acctgatagc ccgcttccgc cagcgcagcc agcggcgccg ctgagagatc gcaggggcga
    10201 ccgaagtaga cgacgcggcg taaaattgac agcatgctag cggctcccgt aggctagacg
    10261 gatggcaggc ttcgcgaaag agagtgactg atggcacgct tggtc
//
LOCUS       DASUBI010000059         9340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1197471, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000059 DASUBI010000000
VERSION     DASUBI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197471"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1178)
                     /locus_tag="VFU72_01055"
     CDS             complement(228..1178)
                     /locus_tag="VFU72_01055"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5422103.1"
                     /translation="MAERHPKYLWWNGRQMPWEDATIHVTEIGWSTVGAIFEGIRAYW
                     NEEQGELYVFRLPEHMQRFAQSMKLVRLDQEISTKDLCDAIVGLLKANESREDTYIRP
                     LAYRGGDGKSFSSFSSEAHILINTRPHPSHLLSGRAQNMGVSSWTRISDNVMPPRIKN
                     LSNYRNGQLAGMEAHYAGYDGPILLNTLGKVAEGSGSCLMMVRNGKLITPDPTSSILE
                     SITRDALLTMARDDLGVETEERMVDRTELYIADEVFMCGTAAEITPVVSVDRYQVGDG
                     KIGPVTHKLEQLFNDVLRGKTNKHADWRTPVGFPSLVAAD"
     gene            complement(1262..3019)
                     /locus_tag="VFU72_01060"
     CDS             complement(1262..3019)
                     /locus_tag="VFU72_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5422104.1"
                     /translation="MSRLSRRRLLAGSLALLLGACRRTEANSPRAIEPGTQPALPTPF
                     SFRPTVPARTPTPTPTPTPRAAPNATPAPPEEQFLLRGTFFADPLSHDFNANIDCGGD
                     ATLWAGLLAVDQQYNLVADWAASWESNADASQWTFHLRPNTGWSDGKPVTAADFVWSW
                     RRALDPATHAPHAWVFFDVVNAAEINRGQLSPDKLGVAALDDLTLRVSLTGPRIYFPA
                     IAASLAAVPAYRPAVEQHGEKWTEAANIVTNGPFKLQAWEHGKQLSTVRSPSYWNAAA
                     ISLRRTLAPILSPEQHLAPYRTNQVDFLPVQAADLAELRGDDSLVAEVHPAVLPATWL
                     LLPSSVPPAGDPLVRQALGHALDRKRLVEVTMGQVIRASSLLPPGFPGASDDPGLEAL
                     QAFDVDAALAARAKTPFAAGDWPPLRLAVEQDDTLAGALGQDVAAQLRENLGMALTVV
                     PLEAGKLAGAPPPDIHLRLLRWGFLYPDPNNGYADALFPPGDETTRLDPEPAGVRALV
                     AQARGERDPSARTALYLEIERRLQQSFAYLPLAHPVQYLLVHAWVQRFPARGGFLIPP
                     SRLFTRLVALLSIAGRPHG"
     gene            complement(3016..3444)
                     /locus_tag="VFU72_01065"
     CDS             complement(3016..3444)
                     /locus_tag="VFU72_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422105.1"
                     /translation="MSEIDYRDSPQGVTPADLTGFFVGWAQRPSPETHLRLLRQSDAV
                     LLAVERGSGRVVGFITALTDGVLSAYIPFLEVLPAFQGRGIGGELLRRLLARLEPLYM
                     IDLVCDPELQPYYERVGMQRAQAMIRRDRAAQAGPPERSG"
     gene            complement(3441..4415)
                     /locus_tag="VFU72_01070"
     CDS             complement(3441..4415)
                     /locus_tag="VFU72_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5422106.1"
                     /translation="MSVTEHVIAVDLGGTNIRAAVVTRTGQIRHLLSQPTLADDGPDA
                     VIDRIADAIAAVVSAEDVAPEVAVGVVAPGPLDPVSGVVFFGPNLPGWHDVPLKEILT
                     RRTGRRVLVGNDANCAALGEAHFGAAQGIRNLIYIALGTGVGGGVIAEGRLIEGARGL
                     GGELGHVPVAIDGPRCTCGGIGCIEAFAGGWAIAREGELVAHSDRSTEIARLAAGKPV
                     TARMVAQAARHGDAAARAVYERAGQALGVGLGGLVNVFNPEMIVVGGGLAEAGDLILD
                     PMRAELPRRAMRQIYPGLRIERSSLGTNTGLYGAAARVFYQQEIAEAP"
     gene            complement(4439..5131)
                     /locus_tag="VFU72_01075"
     CDS             complement(4439..5131)
                     /locus_tag="VFU72_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5422107.1"
                     /translation="MKTLSIVIPVYNEAGTIREIVRQVRAVELPYQRQIIVVDDASSD
                     GTRDMLAAEAAEDAALTVLYHAVNQGKGAAVRTGLRQASGDIVLIQDADLEYDPRDYP
                     RLLRPIEEGRSQVVYGSRFLGEHKAMYFWHALGNKLLTLTTNVLFDTTLTDMETCYKV
                     FTAEVAGKIRLKSDRWGFDPEITAKILKRGYRIYEVPIAYNGREFWEGKKISWRDGFT
                     VLLTLIRYRLVG"
     gene            complement(5549..6994)
                     /gene="hslU"
                     /locus_tag="VFU72_01080"
     CDS             complement(5549..6994)
                     /gene="hslU"
                     /locus_tag="VFU72_01080"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU5422108.1"
                     /translation="MSTKQGSETPAPIAPISPRERELTPRRIVAELDRFIIGQDEAKR
                     AAAIALRNRWRRQQLDEAMRPEVTPKNILMIGPTGVGKTEIARRLARIVDAPFLKVEA
                     TKFTEVGYVGRDVEAIVRDLLDIAINMLHDERLEAVQDEAAVAAMQRLADILVEQRAS
                     STSRAVARGKDGTPVAEESPRQRAAATRRRQAQRRRMLELLSGNALEDETVELELSDD
                     LDVYVSQAEFTSPEEAREGFGELLDGLLSRRRSRRVSVREARRILTQQEAYRLIDFDT
                     VIESAVRRTEEAGIVFIDEIDKTITNSDAVGSEVSGEGVQRDLLPIVEGSVVVTRYGP
                     VRTDHILFVAAGAFSDTRPSDLIPELQGRFPIRVELASLEEEDLYAILTQPENALTKQ
                     YRQLLATEGVAVEFTDDGMREMARLAWEINERGEDIGARRLHTIVERVLEEISFEAPE
                     RAGEQIVIDGDYVRARIEEVAADDELSNFIL"
     gene            complement(6972..7538)
                     /gene="hslV"
                     /locus_tag="VFU72_01085"
     CDS             complement(6972..7538)
                     /gene="hslV"
                     /locus_tag="VFU72_01085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU5422109.1"
                     /translation="MSERWHGTTIIGVRRDGTTAVAGDGQVTFGDVVMKHGAHKVRRL
                     AGGRVVAGFAGAVADALTLLEKFEVHLKDESGNLRRAAVALAKEWRTERYLRRLEAQL
                     IIADSDHLLLMSGEGDVLEPDAAVVAIGSGGPFAAAAARALLAHSQLSAEEIARAAMR
                     IAADLCIYTNDTITIEVVTHEDEHETGE"
     gene            complement(7541..>9340)
                     /gene="topA"
                     /locus_tag="VFU72_01090"
     CDS             complement(7541..>9340)
                     /gene="topA"
                     /locus_tag="VFU72_01090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5422110.1"
                     /translation="PEAIKHALSEPRDLDMRLVDAQQARRVLDRLVGYEISPLLWRNV
                     KRGLSAGRVQSVALRLIVEREREIRGFVSEEYWTLDAELAKREAGRKKAASFLATLSR
                     VDGEKPELRTEAQTTAIVDALQGAEFVVAGVQTKETQRRPAAPFTTSTLQQEASRKLR
                     LPVRRTMQLAQELYEGVELGQGGTQGLITYMRTDSSNVAGSAQARAREVITQRYGQQY
                     LPERAPVYARRAKGAQEAHEAIRPTDPARTPEAVQPFLSQPQYRLYRLIWQRFIASQM
                     RNAIFDSTAVDVDAGQPGGAKPYRFHATGSVIKFKGFIEVYREGRDDEELDDLDRAAL
                     PPLVAGEPLDLQQLIPEQHFTQPPPRYTEASLVKSLEELGIGRPSTYAPTIATLEARY
                     YVTKAERKLTPTELGEIVNDLLVAHFPEIVDVQFTSRMEDELDEIASGERAWVPVMRD
                     FYGPFHQTVEQASQQMTRVKVADEATDEVCEKCGRPMVIKLGRFGRFLACSGFPECRN
                     TKPLLEKIGVTCPQCGEGELVVKRARKPVKGRSPIFYGCSRYPDCNFTSSEKPTGERC
                     PACGDLLVETARGAIRCRNSQCSWRGPRLEKAS"
BASE COUNT         1366 a         3229 c         3231 g         1514 t
ORIGIN      
        1 gagagggaag gtgatcgggt ccggcttacc ggcgggttcc tgctccccga tgcgcgtctc
       61 gtcgtccatc gcattcccac ccgaacagcg gctcgtgggt tgggagtata cgtgttcgta
      121 caccgtgcca ccagacacag gctctcacgc gcggagactc cctggccccc tctcccaatg
      181 ccgggagagg gggacggaga gcttgcgtcg cactgtgcga gtcggagcta gtcggcggcg
      241 acgaggctgg ggaagccaac cggcgtgcgc cagtcggcgt gcttgttggt cttgccacgc
      301 agcacgtcgt tgaagagttg ctccagcttg tgcgtgaccg gcccgatctt gccgtcgccg
      361 acctgatagc ggtcgacgga gacgaccggg gtgatctcgg ccgcggtgcc gcacatgaag
      421 acctcatcgg cgatgtagag ctcggtgcgg tcgaccatgc gctcctcggt ctcgacgccg
      481 agatcgtcac gcgccatggt caagagggca tcgcgggtga tgctctccag gatgctgctg
      541 gtcgggtcgg gcgtgatcag cttgccgttg cgcaccatca tcaggcagga gccggagccc
      601 tcggccacct tgcccagcgt gttgagcagg atcgggccgt cgtagccggc gtagtgggcc
      661 tccatgcccg ccagttggcc gttgcggtag ttcgacaggt tcttgatgcg cggcggcatg
      721 acgttgtccg agatgcgcgt ccaggaactg acgcccatgt tctgcgcccg gccgctgagc
      781 aggtgcgacg ggtgcggacg ggtattgatc aagatatgcg cttccgagct gaaggagctg
      841 aagctcttgc cgtcgccgcc gcgataggcc agcgggcgga tgtaggtgtc ttcgcgcgac
      901 tcgttcgcct tgagcaggcc gacgatcgcg tcgcagaggt ccttggtgga gatctcctgg
      961 tcgaggcgca ccagcttcat cgactgcgcg aagcgctgca tgtgctccgg aaggcggaag
     1021 acgtagagct cgccctgctc ctcgttccag taggcgcgaa tcccctcgaa gatcgcgccg
     1081 actgtcgacc agccgatctc ggtgacgtgg atggtggcgt cctcccacgg catctgcctg
     1141 ccgttccacc acaggtattt gggatgtcgc tcggccatcg attgacctcc ttgcgtccgt
     1201 gccgcgagcc gctcacggca cgacccctct atcgttgcat gtcgcaggat agcacatttc
     1261 cctacccgtg cggacggccg gcgatgctga gcagcgcgac gagccgcgtg aagagccggc
     1321 tgggcgggat caggaaaccg ccacgggcgg ggaagcgctg cacccaggca tggaccagga
     1381 ggtattgcac cggatgggcc agcggcagat aggcgaacga ctgctgcagg cggcgctcga
     1441 tctccaggta gagcgcggtt cgcgcggacg ggtcgcgctc gccgcgtgcc tgcgcgacca
     1501 gggcgcgcac gcccgccggc tcgggatcga gccgggtcgt ctcgtcaccg ggcgggaaga
     1561 gagcgtcggc gtagccgttg ttgggatcag gatacaggaa gccccagcgc agcagccgca
     1621 ggtggatatc cggcggcggt gcgcccgcga gcttgccggc ctccagcggc acgaccgtca
     1681 gcgccatgcc gaggttctcg cgcaactgcg ccgcgacgtc ttgccccaac gccccggcca
     1741 gggtgtcgtc ctgctcgacc gcgagccgca gcggcggcca gtcgcccgcg gcaaacggcg
     1801 tcttggcccg ggccgccagc gcggcgtcga cgtcgaaggc ctgcagcgct tcgaggccgg
     1861 ggtcgtcgct ggcgccgggg aagcccggcg gcagcaggct gctcgcgcgg atcacctggc
     1921 ccatggtgac ctcgaccagc cgcttgcgat cgagcgcgtg gccgagcgcc tggcgcacca
     1981 gcggatcgcc ggccggcggt acgctggacg gcagcagcaa ccaggtggcc ggcaagaccg
     2041 ccgggtggac ctcggcgacc agcgagtcat ccccacgcag ctcggccagg tcggcggcct
     2101 gcaccgggag aaaatcgacc tgattggtgc gatagggggc caggtgctgc tccggcgaga
     2161 ggatcggcgc gagcgtgcgc ctgagcgaga tcgcggcggc gttccagtag gaagggctgc
     2221 gaacggtgct gagctgtttg ccgtgctccc aggcttgcag tttgaacgga ccgttggtga
     2281 cgatgttcgc ggcctcggtc cacttctctc catgctgctc aaccgccggt cgataggccg
     2341 gcaccgcggc cagcgaggcg gcgatggccg ggaagtagat gcgcgggccg gtcagcgaca
     2401 cccgcagcgt caggtcgtcg agtgcggcca cgcccagctt gtcgggcgag agctggcccc
     2461 ggttgatctc ggccgcattg acgacatcga agaagaccca ggcgtgggga gcgtgcgtgg
     2521 ccggatcgag cgcgcgccgc caggaccaga cgaagtcggc ggcggtgacg ggcttgccgt
     2581 ccgaccagcc agtattcgga cgcaggtgga aggtccactg gctggcgtcg gcgttcgact
     2641 cccagctcgc cgcccagtcg gccaccaggt tgtactgctg atcgaccgcc agcagcccgg
     2701 cccaaagggt ggcgtcgccg ccgcagtcga tgttggcgtt gaagtcgtgg cttagcggat
     2761 cggcgaagaa ggtgccgcgc agcaggaact gctcctcggg cggcgccggg gtcgcattgg
     2821 gcgccgcgcg cggggttggt gtgggcgtcg gtgtcggcgt gcgagcggga accgtcgggc
     2881 ggaacgaaaa gggggtcggc agcgccggct gcgtgcccgg ctcgatcgcg cgcgggctgt
     2941 ttgcctcggt gcggcggcag gcgccgagca gcagcgcgag cgacccggcc agcagccgcc
     3001 ggcggctcag ccggctcacc ccgagcgctc cggcggaccc gcctgggcgg cgcggtcgcg
     3061 gcggatcatg gcctgggcgc gctgcatgcc gacgcgctcg tagtacggct gcagctcggg
     3121 gtcacagacg agatcgatca tgtagagcgg ctccaggcgc gccagcaggc ggcgtagcag
     3181 ctcgccgccg atgccgcgcc cctgaaatgc gggcagaacc tccagaaagg gaatgtaggc
     3241 actgagcacg ccatcggtga gcgcggtgat gaagccgacc acgcggccgc tgccgcgctc
     3301 gaccgccagc agcaccgcgt cgctctggcg cagcaggcgc aggtgcgtct ccggcgaagg
     3361 ccgctgggcc cagcccacga agaaacccgt gagatcggcg ggcgtcacac cctgcggcga
     3421 gtcgcggtag tcgatctcgc tcacggcgcc tccgcgatct cctgctggta gaagacgcgc
     3481 gccgccgcgc cgtagagacc ggtgttggtg cccagcgacg agcgctcgat acgcagaccg
     3541 ggatagatct gacgcatggc gcggcgcggc aactcggcgc gcatcggatc gagaatcaga
     3601 tcgccggcct cggccagccc gccgccgacg acgatcatct ccgggttgaa gacgttgacc
     3661 aggccgccca gcccgacgcc cagcgcctgc ccggcacgct cgtagacggc gcgcgctgcc
     3721 gcatcgccgt gccgcgccgc ctgcgccacc atgcgtgcgg tcaccggctt gcccgcggcc
     3781 agtcgggcga tctccgtcga gcgatccgag tgcgcgacaa gctcaccctc gcgcgcgatg
     3841 gcccagccgc cggcgaaggc ctcgatgcag ccgatgccgc cgcaggtgca gcgcgggccg
     3901 tcgatggcca ccggcacgtg ccccagctcg ccgcccaggc cgcgcgcgcc ttcgatcagc
     3961 cgcccctcgg cgatcacgcc gccgcccact ccggtcccca gtgcgatgta gatcaggttg
     4021 cgtatcccct gggccgcgcc gaagtgcgcc tcgcccagcg cggcgcagtt ggcgtcgttg
     4081 ccgaccagca cgcggcgccc agtgcgtcgc gtcaggatct ccttgagcgg gacgtcgtgc
     4141 caaccgggga gattcgggcc gaagaagacg acgccgctga ccgggtcgag cggaccgggc
     4201 gccactaccc cgaccgcgac ctccggcgcc acgtcctcgg ccgagacgac cgccgcgatc
     4261 gcgtccgcga tgcggtcgat caccgcgtcc ggcccgtcgt cggccagcgt cggctgactc
     4321 aggaggtggc ggatctggcc cgtgcgcgtc accacggcgg cgcggatgtt cgtcccgccg
     4381 agatcgacgg cgatcacgtg ctcggtgaca ctcaccgggc tctccctccc cctttgccct
     4441 agccgaccag gcgataacgg atcagcgtca agaggacggt gaagccatca cgccaggaaa
     4501 tcttcttccc ctcccaaaat tcgcgtccat tataggcgat cggcacttca tagatgcggt
     4561 agccgcgctt gaggatcttg gcggtgatct cggggtcgaa gccccagcgg tcggacttga
     4621 ggcgaatctt gccggcgacc tcggcagtga agaccttgta gcaggtctcc atatcggtca
     4681 gggtggtgtc gaagaggacg ttggtggtca gggtcagcag cttgttgccg agcgcatgcc
     4741 agaagtacat cgccttgtgc tcgcccagga agcgcgagcc gtagacgacc tgcgagcgcc
     4801 cctcctcgat cgggcgcagc agccgggggt agtcgcgcgg gtcatactcc aggtcggcgt
     4861 cctggatgag gacgatatcg ccgctggcct gtcgcaggcc ggtgcgcacg gcggccccct
     4921 tgccctggtt gaccgcgtgg tagagcacgg tgagggcggc atcctcggcc gcttcagcgg
     4981 ccagcatgtc gcgcgtgccg tcgctggagg cgtcatccac cacgatgatc tgccgctggt
     5041 agggcagctc caccgcgcgt acctgccgca cgatctcgcg gatcgtccca gcctcgttgt
     5101 agaccgggat cacgattgag agggtcttca cgctgctcgt ccccctatgg acccttgtgt
     5161 acggctcccg ctccttgact ccattacgtg gcgggcgagg acggatcctt cgactgcgct
     5221 caggatgaca ggggaaaggg gatgacctac cggacgaggc gcatcgtcag ccgacaccga
     5281 tcctcttcgt ccgcctcatc cttaaccctg tcatcctgag cttgtcgctc atctttatac
     5341 acatcctggt cgtaccgatt gagggcgctg tggcagctcc ggtcgtacat ggcccgggcc
     5401 tgcgaggcgg caggcgaagc ggcacaggcg gcgtctcgtt cgtatgggct acgtacacgt
     5461 tgtgtataaa gatgagagct tgtcgaagga tccgtcccca ctcactccga tgcccctcga
     5521 ctggcacgcc tttcccccaa cacacctgct acagaatgaa gttgctcagt tcgtcgtcgg
     5581 cggcgacctc ctcgatgcgg gcgcggacgt agtcgccgtc gatgacgatc tgctcgcccg
     5641 cccgctcggg cgcctcgaag ctgatctcct ccagcacccg ctcgacgatg gtgtgcagcc
     5701 gccgcgcgcc gatatcctcg ccgcgctcgt tgatctccca ggccagccgc gccatctcgc
     5761 gcataccgtc gtcggtgaac tcgaccgcca caccctcggt cgccagcaat tgccggtact
     5821 gcttggtcaa ggcattctcc ggctgcgtca gaatcgcgta gagatcctct tcctccaggc
     5881 tggccagctc gacccggatc gggaagcggc cctgcagctc ggggatcagg tcggatgggc
     5941 gcgtgtcgct gaaggcgccc gccgcgacga agaggatatg gtcggtgcgc accgggccat
     6001 agcgcgtcac cactaccgat ccttcgacga tcggcagcag gtcgcgctgc accccctcgc
     6061 cggagacctc gctgccaacc gcgtcgctgt tggtgatcgt cttgtcgatc tcatcgatga
     6121 agacgatgcc cgcttcttcg gtgcgccgga ctgccgactc gatcaccgta tcgaagtcga
     6181 tcaggcggta ggcctcctgc tgggtcagaa tgcgccgtgc ctcgcgcacc gacacgcgcc
     6241 gcgagcggcg acggctgagc agcccgtcga gcagctcgcc gaaaccctcg cgcgcctcct
     6301 ccggggaggt gaactcggcc tgcgagacgt agacgtcgag atcgtcggac agctccagct
     6361 cgacggtctc atcttccagc gcgttgccgg agagcagctc cagcatccgg cgacgctgcg
     6421 cctgccggcg gcgcgtggcg gcggcccgtt gccgcggcga ctcctccgcc acgggcgtgc
     6481 cgtccttgcc gcgcgccacc gcgcggctgg tgctcgaggc gcgttgctcg accaggatgt
     6541 cggccaaccg ctgcatggcg gccaccgccg cctcgtcctg caccgcctcc agccgctcgt
     6601 cgtgcagcat gttgatggcg atgtccagca ggtcgcgcac gatcgcctcg acgtcacgcc
     6661 cgacgtagcc gacctcggtg aacttggtcg cctcgacctt gagaaacggc gcgtcgacga
     6721 tgcgggccag gcgacgggcg atctcggtct tgccgacgcc ggtcgggccg atcatgagga
     6781 tattcttcgg cgtgacctct ggccgcatgg cctcgtcgag ctgctgccgc cgccagcggt
     6841 tgcgcagcgc gatcgccgcg gcgcgcttgg cctcgtcctg gccgatgatg aagcggtcca
     6901 gctcggccac gatgcggcgc ggcgtcagct cgcgctcgcg gggactgatg ggggcgattg
     6961 gggctggggt ctcactcccc tgtttcgtgc tcatcctcgt gtgtaactac ctcaatcgtg
     7021 atggtgtcgt tcgtgtagat acagaggtcg gcggcgatgc gcatggcggc gcgggcgatc
     7081 tcctcggcgc tgagctggct gtgggccagc aacgctcggg cggccgccgc cgcgaacggt
     7141 cctccgctgc cgatggcgac cacggcggcg tcgggctcga gcacgtcgcc ctcgcccgac
     7201 atcagcagca ggtggtcgct gtcggcgatg atcaactgcg cttccaggcg acgtaggtag
     7261 cgctcggtgc gccactcctt agccagcgcc accgccgcgc ggcgcaggtt gccgctctcg
     7321 tccttcagat gaacttcgaa cttctccagc aacgtcagcg cgtcggcgac cgcgccggcg
     7381 aagcccgcca cgacacgccc gccggccagg cggcgcacct tgtgcgcgcc gtgcttcatc
     7441 accacgtcgc cgaaggtgac ctggccgtcg ccggccacgg cggtcgtgcc gtcgcgccgg
     7501 acgccgataa tggtcgtgcc gtgccagcgc tcgctcaccg ctagctcgcc ttctccagcc
     7561 ggggtccacg ccagctacac tggctgttgc gacagcggat ggcgccgcgc gcggtctcca
     7621 ccagcaagtc gccgcaggcc gggcagcgct ccccggtcgg cttctccgag ctggtgaaat
     7681 tgcagtccgg gtagcggctg cagccgtaaa agatcgggct gcggcccttg accggcttgc
     7741 gcgcccgctt gaccaccagc tcgccctcgc cgcactgcgg gcaggtgacg ccgatcttct
     7801 ccaagagcgg cttggtgttg cggcactcgg ggaagccgct gcaggccagg aagcgtccga
     7861 agcggcccag cttgatgacc atcggacggc cgcacttctc gcagacctca tcggtcgcct
     7921 cgtcggccac cttgacccgc gtcatctgct gcgatgcctg ctcgacggtc tggtggaacg
     7981 gcccgtagaa gtcacgcatc accggcaccc aggcgcgctc gcccgacgcg atctcgtcca
     8041 gctcgtcctc catgcgcgag gtgaactgca cgtcgacgat ctcgggaaaa tgcgccacca
     8101 gcagatcgtt gacgatctcg cccagctcgg tcggcgtcag cttgcgctcc gccttggtga
     8161 catagtagcg cgcctccagc gtggcgatgg tcggcgcgta ggtgctcggc cggccgatgc
     8221 cgagctcctc cagcgacttg accaggctgg cctcggtata gcggggcggc ggctgggtga
     8281 agtgctgctc cgggatgagc tgctgcagat ccagcggctc gcccgcgacc agcggcggca
     8341 gcgccgcgcg gtcgaggtcg tccagctcct cgtcgtcgcg cccctcgcgg tagacctcga
     8401 tgaagccctt gaacttgatc accgagccgg tggcgtggaa gcggtagggc ttggcgccgc
     8461 ccggctgccc ggcgtcgacg tcgaccgcgg tcgagtcgaa gatggcgttg cgcatctggc
     8521 tggcgatgaa gcgctgccag atcaggcggt agagccggta ctgcggctgg ctgaggaacg
     8581 gctgcacggc ctccggcgtg cgggccggat cggtcggccg gatcgcctcg tgcgcctcct
     8641 gcgcgccctt ggcccggcgc gcgtagacgg gcgcgcgctc gggcaggtat tgctggccgt
     8701 agcgctgggt gatgacctcg cgggcacgcg cctgcgccga gccggccacg ttggacgagt
     8761 cggtgcgcat gtaggtgatc agcccctgcg tcccgccctg ccccagctcg acaccctcgt
     8821 agagctcctg cgccagctgc atggtgcgcc gcaccggcag gcgcagcttg cgcgaggcct
     8881 cctgctgcag cgtgctggtg gtgaagggcg ccgccggccg ccgctgcgtc tctttggtct
     8941 gcacgccggc gaccacgaac tccgcgccct gcagcgcgtc gacgatcgcc gtcgtctgcg
     9001 cctcggtccg aagctccggc ttctcgccgt cgacgcgcga cagcgtcgcc aggaagctcg
     9061 ccgccttctt gcggccggct tcccgcttgg ccagctcggc gtcgagcgtc cagtactcct
     9121 ccgacacgaa gccgcggatc tcgcgctcgc gctcgacgat cagccgcaac gcgaccgatt
     9181 gcacgcgccc tgccgagaga ccgcgcttga cgttgcgcca gaggagcggg ctgatctcgt
     9241 agcccaccag gcgatcgagg acacgccgcg cctgctgcgc gtcaaccagg cgcatgtcga
     9301 gatcgcgcgg ctcgctcagc gcgtgcttga tcgcctcggg
//
LOCUS       DASUBI010000060         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_338810, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000060 DASUBI010000000
VERSION     DASUBI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338810"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1200
                     /locus_tag="VFU72_01095"
     CDS             154..1200
                     /locus_tag="VFU72_01095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5422111.1"
                     /translation="MQASQLDPRQSDALTATLLGHAEDDATIRSLSLVVPVHDEVDSL
                     PALYTELLEAITPLGLDWELIFVDDGSRDGSVRVLRELHEADPRVQLIELRRNFGKSA
                     ALAAGFAAARGQAVVTLDSDLQDVPAEIPRLLAELEAGADLVSGWKFPRHDPPGKRLP
                     SSFFNLVVRLTTGVALHDFNCGLKAYRAEVLEEVQVYGELHRYIPVLAHFRGFRVAEV
                     KVSHRPRRYGRSKFGRGRFARGFFDLLTVLFLTQYTRRPLHFFGWFGLGALLLGFLIN
                     AYLAAEWFLGQPIGHRPLLTLGVLLMIVGAQFMLFGLLAEMLASTRGGHDYSVRRHAV
                     RRRLPLPLPPRDVD"
     gene            1190..2107
                     /locus_tag="VFU72_01100"
     CDS             1190..2107
                     /locus_tag="VFU72_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422112.1"
                     /translation="MSIDLPLEHVVCDLCGADDPEPLLTLHDVLAGTTPLPFQLVRCR
                     RCGLRYLSPRPCAEALPTIYPADYAPFRQSGLSARIKRRGRRRELAALRPLLDPPRRV
                     LDLGCATGELLQIVRELGNADVLGVEPSSEAAAVARARGLEVVAGTLEDARLPSGSLD
                     AALLAHVIEHLPSPDATLAELARVLRPGGALVLWLPNADSLAARLLGARWIGYDAPRH
                     LYAFTPRTLGSLLARHDFTVRSVEHEWIGLEWSWALRLWAAERSPRLGRMLARLHPLT
                     TAALTPLSAAAATLRRAGRVRVVAVKGVR"
     gene            complement(2320..2820)
                     /locus_tag="VFU72_01105"
     CDS             complement(2320..2820)
                     /locus_tag="VFU72_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5422113.1"
                     /translation="MQKPEVTNHEAALILLNIASVIEMLAGNPYRVRAYRRAAQMLLR
                     LRRPATEYLTPEGELALPMLGASLRRKLGELFARGEMQFYRDLYASLPPEMVRLMEVR
                     GIGPRIALRLYVELGIGSAEELIAAAERGQIRRLYRFGEVSEAKLAAAARELLPASEP
                     ALPVAA"
BASE COUNT          386 a         1046 c         1000 g          467 t
ORIGIN      
        1 agccgtcgag gaagaccgct ccgtcgcggt tgaccacgat cgccgcccac tcgccggcca
       61 cctcgcctcc tcccggcgct ctttcgcgcg cgtgctacac tcacgcgagc gctgggcaca
      121 gtctagcctg gatgacgacc acgagccggg gatatgcagg cgagccagct cgatcccaga
      181 caatccgacg cgctgacggc cacgctgctg ggccacgcgg aagacgacgc caccattcgc
      241 tcactgtcgc tggtggtgcc ggtccacgat gaggtcgata gcctgccggc gctctatacg
      301 gagctgctcg aggcgatcac gccgctcggc ctcgactggg agctgatctt cgtcgacgat
      361 ggcagccgcg acggctcggt gcgcgtgctg cgcgagctgc acgaggccga cccgcgcgtg
      421 caactgatcg agctgcggcg caacttcggc aagtcggccg cgctggccgc tgggttcgcc
      481 gccgcgcgtg gccaggcggt ggtgacgctc gactccgacc tgcaggacgt gccggccgag
      541 atcccgcgcc tgctggccga gctggaggcc ggcgccgacc tggtcagcgg ctggaagttc
      601 ccgcgccacg acccgcccgg caaaaggctt ccctccagct tcttcaatct ggtggtgcgg
      661 ctgacgacgg gtgtcgcact gcacgacttc aactgcggcc tgaaggcata ccgggccgag
      721 gtactggagg aggtgcaggt ctacggcgag ctgcaccgct acatcccggt gctggcgcac
      781 ttccgtggct tccgcgtggc cgaggtcaag gtcagccacc gcccgcgccg ctacgggcgc
      841 tccaagttcg ggcgaggccg cttcgcgcga ggtttctttg acctgctgac cgtgctcttc
      901 ctgacccaat acacacgccg gccgctgcat ttcttcggtt ggttcggcct gggcgccctg
      961 ctgctcggct tcctgatcaa cgcctacctg gcagccgagt ggttcctggg ccagccgatc
     1021 ggccatcggc cgctgctgac gctgggcgtg ctgctgatga tcgtcggcgc tcagttcatg
     1081 ctcttcgggc tgctggccga gatgctcgcc agcacgcgcg gcgggcacga ctactcggtg
     1141 cgccgccacg ccgtgcgccg gcgcctaccc ctgccgctgc cgccgcgcga tgtcgattga
     1201 tctccccctg gagcacgtcg tctgcgatct ctgcggcgcc gacgaccccg agccgctgct
     1261 gacgctccat gatgtactgg ccgggaccac cccgctcccg ttccaactcg tgcgctgccg
     1321 ccgctgcggg ctgcgctatc tatcgccgcg accgtgcgcc gaagccctgc caaccatcta
     1381 cccggccgac tacgcgccct tccggcaatc cggtctgtcg gcccggatca agcggcgcgg
     1441 ccggcggcgc gagctcgccg cgctccggcc actgctggac ccgccgcgac gggtgctcga
     1501 cctcggctgc gccaccggcg aactcttgca gatcgtgcgc gagctgggga acgccgacgt
     1561 gctcggcgtc gagccgagct ccgaggcggc ggcggtggcc cgcgcgcgcg ggctggaggt
     1621 ggtggccggc acgctggagg acgcgcgtct cccctccggc tcactcgacg ccgccctgct
     1681 cgcccacgtc atcgagcacc tgccctcgcc cgacgccacg ctggcggagc tagcgcgcgt
     1741 gctgcgcccc ggcggcgcgc tcgtgctctg gctgcccaac gccgactcgc tggccgcccg
     1801 gctcctgggc gcgcgctgga tcggctacga cgccccgcgc cacctctacg cctttacccc
     1861 gcgcaccctt ggcagcctgc tggcgcgaca tgacttcacg gtgcgctcgg tcgagcacga
     1921 gtggatcggg ttggagtggt cctgggcgct gcgcctgtgg gcggccgagc gcagcccgcg
     1981 gctcggtcgc atgctggcgc ggctgcaccc actcaccacg gccgcgctca caccgctgtc
     2041 ggcggctgcc gctacgctta gacgtgcggg tcgggtgcgg gtggtggcgg tgaagggcgt
     2101 gagatgagag gagaagggga cggcggcatc tgccgagtgg attgggtgtc gctcggcgcg
     2161 cggggctgaa gccaccgcgc tagactctta cgcaagcctg ctgaagcagg ctaaggtttg
     2221 aactcagcct gcttcagcag gcttcgggag ggctagcgcg gcggctatac cccgcgcgac
     2281 ccccgcgccg cgattccgtc atcggcggta cagtcggcct tacgccgcca ccggcagcgc
     2341 cggctccgac gccggcagca gctcgcgggc ggcggcagcc agcttggcct cgctgacctc
     2401 gccgaagcgg tagaggcggc gaatctggcc gcgctcggcg gccgcgatca gctcctcggc
     2461 cgagccgatg cccagctcga cgtagagccg cagcgcgatg cgcgggccga tgccgcgcac
     2521 ctccatcagc cgcaccatct ccggcggcag gctggcgtag aggtcgcggt agaactgcat
     2581 ctcaccgcgc gcgaagagct cgcccagctt gcggcgcagc gaggcgccca gcatgggcag
     2641 cgccagctcg ccctccggcg tcaggtactc cgtggccggg cggcgcaggc gcagcagcat
     2701 ctgcgcggcg cggcggtagg cgcgcacgcg gtagggattg ccggccagca tctcgatgac
     2761 cgaggcgatg ttgagcagga tcagggcggc ttcgtggttg gtgacttctg gcttctgcat
     2821 ggcttgctcc acggattcga atagttgtcc caagtatacc atttatgttg agttggcgcc
     2881 gaaaaacttg actctgtga
//
LOCUS       DASUBI010000061         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_488989, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000061 DASUBI010000000
VERSION     DASUBI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488989"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VFU72_01110"
     CDS             <1..1282
                     /locus_tag="VFU72_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5422114.1"
                     /translation="AATAFNRARVIGGCSAHNGCVALAGHRRDYDHWAELGNPGWEYD
                     AVAPAFERARRRLRVRIPGDDEMTPYQAAFVAAAAAAGIPRVANLDEPDDDTGVGVSP
                     ANIAEGVRWNTAFAYLDPVRSRPNLTVRGGALVDRVIVEGGRAGAVEAIVDGRREVLR
                     AGRIVLAAGAYGSPAILLRSGIGPAAELRGLGIAPVHDLPGVGRGLTDHPTTYLVFRG
                     SAALNAAMAGFTGWLPDEQTLLKARSSRCREAFDLHLYAVSGRDETTGQPYYVVDISC
                     VAPRSFGSLRLASAEPGAAPLIDHGYLSDPEGSDAAVLADGVELARAIAGNAPLANLL
                     LEEVRLGSGPKARGEIADFVRRTIGIYYHPACSCRMGPTSDPLAVVDAGGRVHGLDNL
                     FVCDASIFPVIMRANTNLPAAMLAEQLAESIGLF"
     gene            1566..2033
                     /locus_tag="VFU72_01115"
     CDS             1566..2033
                     /locus_tag="VFU72_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5422115.1"
                     /translation="MQDPHELVKLSDSGLTVADPAEDVRHRDVIDRDGDKIGTVDDLY
                     IDTKERRVRLLDVGSGGFLGIGEQKVLIPIDDITRLDPDHVYINQSRQFVGKGPTYDP
                     RLAPDRTYANTLYSYYGYSPYWAPGYLPPPFPNYPISQAERQTDASREAEAES"
     gene            2030..2374
                     /locus_tag="VFU72_01120"
     CDS             2030..2374
                     /locus_tag="VFU72_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422116.1"
                     /translation="MMEQHPEGTALSGQSNERCPACGAPADLGSLSSNTDARALRRQA
                     DHLRLLRAELEREQITPLQVEQMLPRLCSSCQGMLTGLGRLAGKGEIESRIQDQIDTL
                     DEQVRLLSRQQG"
     gene            complement(2387..>2579)
                     /locus_tag="VFU72_01125"
     CDS             complement(2387..>2579)
                     /locus_tag="VFU72_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU5422117.1"
                     /translation="RVEALIAEVKAAPLAPGQTEIFYPGEPEARAEARNARLGIPLPA
                     KTVAELEALAGELALASPF"
BASE COUNT          384 a          930 c          897 g          368 t
ORIGIN      
        1 ggcagccacc gccttcaatc gtgcgcgggt gatcggcggc tgctcggcgc acaacggctg
       61 cgtggccctg gccgggcacc ggcgcgacta cgatcactgg gccgagctgg gcaaccccgg
      121 ctgggagtac gacgcggtcg cacccgcctt cgagcgcgcc cggcgccggc tgcgggtgcg
      181 catccccggc gacgacgaga tgacgcccta tcaagccgct ttcgtggccg ccgccgctgc
      241 cgccggcatc ccgcgcgtgg ccaacctcga cgagccggac gacgacacgg gtgtcggcgt
      301 ctcgccggcc aacatcgccg agggtgtgcg ctggaacacg gccttcgcct accttgaccc
      361 ggtgcggtcg cggccgaacc tgacggtgcg cggcggcgcg ctggtcgatc gcgtcatcgt
      421 agagggcgga cgcgcggggg cggtcgaggc gatcgtcgac gggcggcggg aggtgctgcg
      481 cgccgggcgc atcgtgctcg cggcgggggc ctacggctcg cccgccatcc tgctgcgctc
      541 cggcatcggg ccggcggctg agctgcgcgg gctcggcatc gcgccggtcc acgacctccc
      601 cggcgtcggg cgcggcctga ccgaccatcc caccacctac ctcgtctttc gcggctccgc
      661 cgcgctcaac gccgccatgg ccgggttcac cggctggctt cccgacgagc agacgctgct
      721 caaggcgcgt agcagccgct gccgcgaggc cttcgacctg cacctctacg ccgtgagcgg
      781 ccgcgacgag accacgggcc agccgtacta cgtcgtcgac atctcctgtg tcgcgccgcg
      841 ctcgttcggg tcgctgcgct tggcctcggc ggagcccggg gccgccccgc tgatcgacca
      901 cggctacctg agcgatccgg aggggagcga cgccgcggtg ctggccgacg gcgtggagct
      961 ggcgcgcgcc atcgcgggga acgcgccact ggcgaacctg ctgctggagg aagtgcggct
     1021 tggttccggc ccgaaggcgc gcggcgagat cgcggatttc gtgcggcgca ccatcggcat
     1081 ctactaccac ccggcctgta gctgccgcat ggggccgacg agcgatccgc tggccgtggt
     1141 cgacgccggc gggcgggttc acgggctgga caatctcttc gtctgcgacg cctcgatctt
     1201 cccggtcatc atgcgcgcca ataccaacct ccccgccgcc atgctggccg agcagctggc
     1261 ggagtcgatt gggctgttct agcggggagg ggtgcccttc gctagcattg ggtggcgggt
     1321 gaggacggat ccttcgactg cgctcaggat gacaagtgga tgggatgagg cgtacgacga
     1381 cgactagcct cgtccgacag gccgacgccc ttgaccctgt catcctgagc ttgtcgaagg
     1441 atccgtcctc gctcagcgcc caacgtcctc ggcgggcacg acaaggacca gcatcccccg
     1501 cgcggcacac agcctgcgaa ccacgcgccg gacgattgct cggtgagcgg cgagaggagc
     1561 gcgccatgca ggacccccac gagctggtca agctcagcga ctcgggactg accgtcgccg
     1621 atccggccga ggacgtgcgt catcgcgacg tcatcgaccg cgacggcgac aagatcggta
     1681 cagtcgacga cctctacatc gacaccaaag agcggcgggt gcgcctcctg gatgtcggct
     1741 cgggcggctt cctgggcatc ggcgagcaga aggtactgat cccgatcgac gacatcacca
     1801 ggctcgaccc tgaccatgtc tacatcaacc agtcccggca gttcgtgggc aaggggccaa
     1861 cctacgaccc gcggctggcg cccgatcgca cctacgccaa cacgctctac agctactacg
     1921 gctactcgcc ctactgggcg ccggggtact tgccgccacc cttccccaat taccccatca
     1981 gccaagccga gcggcagacc gacgccagcc gcgaagccga ggccgagtcg tgatggagca
     2041 gcaccccgag ggaaccgcgc tgtctggcca atcgaacgag cgctgcccgg cctgtggcgc
     2101 accggccgat ctcggctcgc tctcgagcaa caccgacgcg cgcgcgctgc gacgacaggc
     2161 cgatcacctc cgcttgctgc gggcggagct cgagcgcgag cagatcacgc cgcttcaggt
     2221 ggagcagatg ctgccgcggc tctgctccag ttgccagggg atgctgaccg gccttggccg
     2281 gctggctggt aagggggaga tcgagagccg catccaggac cagatcgaca cgctcgacga
     2341 gcaggtgcga ctgctgagcc ggcagcaggg ctgagcgccg ctcggctcag aacggcgagg
     2401 cgagggccaa ctctccggcc agcgcctcca gctcggccac ggtcttggcc ggcagcggga
     2461 tgcccagccg ggcgttgcgc gcctcggcgc gggcttccgg ctcgccgggg tagaagatct
     2521 ccgtctgtcc aggcgccagc ggcgcggcct tgacctccgc gatcagcgct tcgacgcgc
//
LOCUS       DASUBI010000062         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_488905, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000062 DASUBI010000000
VERSION     DASUBI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488905"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFU72_01130"
     CDS             <1..419
                     /locus_tag="VFU72_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422118.1"
                     /translation="AYLFVDAQLPLAPGGSPEPLPPELLQLLNAGGRYPNWRDDDLRE
                     EIPDPALRARLLRELQPRGRDYFETPVPLPAGWPDAPAAYLRFSPHYAPQFERARALG
                     WPTHELPGGHFQLLVDPAGVSDVLLALAQECRARDS"
     gene            518..1135
                     /locus_tag="VFU72_01135"
     CDS             518..1135
                     /locus_tag="VFU72_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422119.1"
                     /translation="MAALPEAEQAPRRELLGRIAGIVVERPLDQLTSLVDEFVQESAE
                     SGSTATWSLAEARTRNLERLLEDQARLVKETIRAATVRQGMNISRQRLHQLVAQGRLV
                     AIQLQEGAPGLYPFWQFTSSAPVQPVTGLSLLLEAAREAGMDEVELHFFAIVSMLPVD
                     NIQRATPARSRSVPSPRPRLSVDADSARRRGSVTCASSVRASRCR"
     gene            1099..1449
                     /locus_tag="VFU72_01140"
     CDS             1099..1449
                     /locus_tag="VFU72_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422120.1"
                     /translation="MRELRPRVPLSLIDLHDLDVRAAFNLDERISVGDDDEDCQAWAL
                     AIDQQYADVAGIRYRARKAGALVANVFLSADRCASQLEISTAPRLDEIEEIVLNAADH
                     YRLTICFPFKSARP"
     gene            1556..2041
                     /locus_tag="VFU72_01145"
     CDS             1556..2041
                     /locus_tag="VFU72_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422121.1"
                     /translation="MSTGSNDFASPEAKAFWDSIAGTLDSLVGTLEGLSDAQLDWRPP
                     APDTNSLDVLVTHTLGNVNESVLEIFGGQQVHRDRDSEFRAQASGNAAQQLQERWQQL
                     RPRAEAVLAALTPEELHRSYSHPRRGQIGGYDVLLLVATHAAQHLGHAELTRDLLRAQ
                     G"
     gene            2057..2827
                     /locus_tag="VFU72_01150"
     CDS             2057..2827
                     /locus_tag="VFU72_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU5422122.1"
                     /translation="MPFPNFAGKHREDAFFNPEEYVAYLRREGLLPEVTLPSGVILCY
                     RRGLLRRIGELEPIERVRGLPAEFYLLGETGGAVGLCGGFGIGAPAVTAVVEELIALG
                     VRRFVSIGTAGALQPESALGEIIVCDRAIRDEGVSHHYLPPEQYAHASPALSARLHAE
                     LARAGGPLSTGAAWTIDAPYRETTAEARHYQSEGVRAIDMEAAALFAVAAYRGVEVAA
                     AFVVSDSLADLVWQPSLRGPEVQLGLDRLYQAARDALR"
     gene            2906..3364
                     /locus_tag="VFU72_01155"
     CDS             2906..3364
                     /locus_tag="VFU72_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5422123.1"
                     /translation="MSEQDNMALVRRYLEHGFQEAIRGNLDVVHQYFADHYHDHSPLH
                     PEHAGVHGLKEIIADTGQAMPDVRNEVLHIAAAGDLVFVHWQATGTHQQQHQITKHAR
                     HLEPSGAEESVSGINLYRIEGGKFVEGWHYHTVLEYVQALAQGRSAAGSS"
BASE COUNT          521 a         1199 c         1145 g          522 t
ORIGIN      
        1 cggcctatct cttcgtcgat gcccaactgc cgctggcgcc cggcgggtcg cccgagccgc
       61 tgccgccgga gctgctgcag ctcctgaacg ctggcggccg ctaccccaac tggcgcgacg
      121 acgacctgcg ggaggagatc cccgacccgg cgctgcgagc ccggctgctg cgcgagctgc
      181 agccgcgcgg ccgcgactac ttcgagacgc cggtgccgct gcctgccggc tggccggacg
      241 cccccgcggc ctacctccgc ttcagccccc actacgcgcc gcagttcgag cgcgcccgcg
      301 ccctcggctg gccgacccac gagctgcccg gcggccactt ccagctgctg gtcgatccgg
      361 ccggggtaag tgacgtgctg ttggcgctgg ctcaggagtg ccgggcacgc gactcttgac
      421 aggcttgacg cggttgacgt gccatacgtt cgggacatcg gtgcacgagc gttgaggtca
      481 gcgtatgtcg acagtattgg agcaagcgga gcggcggttg gcggcgctgc ccgaggcgga
      541 gcaggcgcct cgccgggagc tactcggccg gatcgccggc atcgtcgttg agaggcctct
      601 ggatcagctg acctccctgg tcgatgaatt cgtccaggag agcgccgaaa gcggtagcac
      661 cgcaacctgg tcgctcgccg aagcgcgcac gcgcaacctg gaacggctcc tcgaagatca
      721 ggcgcgactg gtcaaagaaa cgatccgggc agcgacggtg cgtcagggca tgaacatctc
      781 gcgccagcgc ctccaccagc tcgttgccca ggggcgcctc gtcgccattc agctgcagga
      841 aggggcgccg gggctctacc cgttctggca gtttacgtca agcgcgccgg tccaaccggt
      901 cacgggtctg tcccttctgc tcgaagccgc ccgcgaagca ggtatggacg aggtggagct
      961 gcatttcttc gctatcgttt cgatgcttcc ggtagacaat atccagcgcg ctacacccgc
     1021 gcgctcgcga tcggtgcctt cgccgagacc taggttgagc gtggacgccg actcggcccg
     1081 caggaggggc agcgtcactt gcgcgagctc cgtccgcgcg tcccgctgtc gctgatcgat
     1141 ctccacgacc tcgacgtccg ggccgccttc aatctcgacg agcgaatctc ggtcggagac
     1201 gatgacgaag actgccaggc ctgggccttg gccatcgatc aacagtacgc cgatgtcgcg
     1261 ggcatccgct accgcgcccg caaggcggga gcgctcgtgg cgaacgtctt cctctctgcc
     1321 gaccgctgcg cttcgcaact ggagatcagc accgcgccgc gcctcgatga gatcgaggag
     1381 attgtgctca acgcagccga tcactaccgg ctgaccatct gcttcccatt caagtctgct
     1441 cgcccgtagc agcaatgtca cgctccggct cagactgccc ggccgtcgct tgtgcggtgg
     1501 tcaaccgacg tgcgacactg ggccgggttg gcacgcgacg taggaggtgg acagcatgag
     1561 caccggatcc aacgacttcg cctcgccgga ggccaaagcg ttctgggact cgatcgccgg
     1621 cacgctcgac agcctggtcg gcaccctgga gggcctgagc gacgcccagc tcgattggcg
     1681 cccaccagca cccgatacca acagcctcga cgtgctggtg acgcacacgc tgggcaacgt
     1741 caacgagagc gtgctggaga tcttcggtgg gcagcaggtc caccgcgacc gcgactccga
     1801 gttccgcgcc caggccagcg gcaacgcggc ccagcagctc caagagcgct ggcagcagct
     1861 gcgcccgcgc gccgaggcgg tgctggccgc gctgacgccc gaggagctgc accgcagcta
     1921 ttcccacccg cgccgcggcc agatcggcgg ctacgacgtc ttgctgctgg tcgcgacgca
     1981 cgccgcccag cacctcggcc acgccgagct gacgcgcgac ctgctgcgcg cccagggttg
     2041 aggggcgggg cgcgccatgc ccttccccaa cttcgccggc aagcaccggg aggacgcctt
     2101 cttcaacccg gaggagtacg tcgcctacct gcggcgcgag gggttactgc cggaggtcac
     2161 cctgccctcc ggcgtcatcc tctgctaccg gcgcggcctg ctgcggcgca tcggcgagct
     2221 ggagccgatc gagcgcgtgc gcggcttacc ggcggagttc tacctgctcg gcgagaccgg
     2281 gggagcggtt ggcctctgcg gcggcttcgg tatcggcgcc ccggcggtga cggcggtcgt
     2341 cgaggagctg atcgcgctgg gcgtgcgccg cttcgtcagc atcggcacgg ccggcgcgct
     2401 gcagcccgag agcgcgttgg gcgagatcat cgtctgcgac cgtgccatcc gcgatgaggg
     2461 tgtctcgcac cactacctgc cgcccgagca gtacgcccac gcctcccccg cgctgagcgc
     2521 gcggctgcac gcagagctgg cgcgcgcggg tggcccgctg agcaccggcg cggcctggac
     2581 gatcgacgcg ccctaccggg agacgaccgc cgaggcgcgg cactaccaga gcgagggggt
     2641 gcgcgccatc gacatggagg cggccgccct cttcgccgtg gccgcctacc gtggcgtcga
     2701 ggtcgcggcc gccttcgtgg tcagcgactc gctggccgat ctcgtctggc agccgagctt
     2761 gcgtgggccg gaggtgcagc ttggcctcga ccggctctac caggcggcgc gcgacgctct
     2821 ccgataattc ctcgggttcc gagagtttat aattggcccc gggcctcagt ctcgtcggaa
     2881 ttcgcgcagg gatggagggc gtgtcatgtc cgagcaagac aacatggcgc ttgtccgccg
     2941 ctacctggag cacggcttcc aggaggccat ccgcggcaat ctcgatgtgg tgcatcagta
     3001 tttcgcggat cactaccacg accactcgcc cctgcacccc gagcacgcgg gtgtgcacgg
     3061 gctgaaagag atcatcgccg acaccggcca ggcgatgccg gacgtgcgca acgaggttct
     3121 gcatatcgcg gccgccgggg acctggtctt cgtccactgg caggcgaccg gcacccacca
     3181 gcaacagcac cagatcacca agcacgcgcg ccacctcgag ccgtcgggtg cggaggagtc
     3241 ggtctccggc atcaacctct atcggatcga ggggggcaag ttcgtcgagg gctggcacta
     3301 ccacaccgtg ctcgagtacg tgcaggcgct cgcgcagggc aggagcgcag ccggcagttc
     3361 ctaggcgccg cccatcgaac acgttgg
//
LOCUS       DASUBI010000063         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_883814, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000063 DASUBI010000000
VERSION     DASUBI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883814"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFU72_01160"
     CDS             complement(<1..565)
                     /locus_tag="VFU72_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEU5422124.1"
                     /translation="MSDEARHYELMRELMAIPGPTGQESRVMAWLRERWGGEVERLWT
                     AKGGNLFAHVGGTGPRLLIEGHADEIGFVVRSVDERGFIWLASGQAPRSDVGRRYPVG
                     QPALILGRGAPVEGLFATTSGHILTEQQRQKPNLDFYDLFVDAGAESREEIYARGVYP
                     GAGVIWNPPTRRNGHRVTGKAVDDRFAL"
     gene            786..1067
                     /locus_tag="VFU72_01165"
     CDS             786..1067
                     /locus_tag="VFU72_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422125.1"
                     /translation="MMLKGRGVMDPQTLVEELLRQRDVLRAHTNLVETLSTSIQEYER
                     RYSLSSDQLHAAIEAGQLQETEEVCDWIIDYELLKQRTVGESEGPSRLE"
     gene            1069..1518
                     /locus_tag="VFU72_01170"
     CDS             1069..1518
                     /locus_tag="VFU72_01170"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HEU5422126.1"
                     /translation="MQGYLAAHEAQLERLGRFFVERNGLTIFWQDAVHLRIEGRLSCT
                     GGIFLDVRKVLESNERNQVRAILYKYHAGIEGPQHRPIFRYDNSHTYLREGHPDPFHK
                     HRFDPDTWQEITPPLWIGYHEWPTLADVLEELHAWWLAMRDSLDLPT"
     gene            1699..>2722
                     /locus_tag="VFU72_01175"
     CDS             1699..>2722
                     /locus_tag="VFU72_01175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU5422127.1"
                     /translation="MTTREQTEQFRVERDSMGEMQVPAGARYGASTARAVENFPISDL
                     RFPPEFIRALGLIKWAAAKVNQELGLLGPEKATLIEQAAYEVAEGAFADQFPIDIFQT
                     GSGTSTNMNANEVIANRANELAGQRVVHPNDDVNMGQSSNDVIPTAIHLSASIGVAQR
                     LLPAMRHLEATLDRRAEETAQIAKMGRTHLMDAMPVMLGQEVLGWAGQVRHAMTGINH
                     VLPSLAELALGGTAVGTGINAHPEFASGVARLLAKQTGLSFQESSNHFYAQATMDTAV
                     QLSGQLKAYAAGLLKFANDLRWMNSGPQGGLGEIVLPALQPGSSIMPGKINPVISEAT
                     MMVCAQV"
BASE COUNT          491 a          890 c          883 g          458 t
ORIGIN      
        1 ccagcgcgaa gcggtcgtcg accgccttgc cggtgacccg gtggccgttg cgccgggtcg
       61 gcgggttcca gatcaccccg gcgccggggt agacgccgcg agcgtagatc tcctcgcggc
      121 tctcggcgcc ggcgtcgacg aagaggtcgt agaagtcgag gttcggcttc tgacgctgct
      181 gctcggtcag gatgtggccg gaggtggtgg cgaagagccc ctcgacgggc gcgccgcggc
      241 cgaggatcag cgccggctgg ccgaccgggt agcgccgccc gacgtcgctg cgcggcgcct
      301 gtccgctggc cagccagatg aagccgcgct cgtcgacgct gcgcaccacg aagccgatct
      361 cgtcggcgtg cccctcgatc agcaggcgcg ggccagtacc gccgacgtgg gcgaagagat
      421 tgccgccctt ggcggtccag agtcgctcga cctcaccgcc ccagcgctcg cgcagccagg
      481 ccatcacccg cgactcctgg cccgtcggcc ccgggatcgc catcagctcg cgcatcagct
      541 catagtgccg cgcttcgtcg ctcacgtctc gttcctcctg cagttcacaa attgctggcg
      601 tcaggcgctc ttgccaccgt ccgtgcgggg ccggaacctc ccgcatgtcg acgacggctg
      661 aacaggtttg ggctttgctg cagtaccttc tcctccagga aatctaacac caacgctcgt
      721 tgaaatcgac gtgctgcccg cgtatgatac aggttgccac gtaagcggag catggaggag
      781 ggtgcatgat gctcaagggc cgaggggtga tggaccctca gacgctcgta gaggaattgc
      841 tccggcaacg agatgtgctt cgcgcgcata ccaatctcgt agaaacgctg tctacgtcga
      901 tccaggaata cgagcggcgc tactcgctgt cctcggacca gcttcatgcc gctattgagg
      961 ctggtcaact gcaagagacc gaggaagtct gcgactggat catcgattat gaactgctca
     1021 agcagcgcac cgtcggggag tccgaaggac cgtcacggct ggaatagctt gcagggctat
     1081 ttggctgccc atgaggccca gcttgagcgg ctgggccgtt tttttgtaga gcggaacgga
     1141 ctcacgatct tttggcagga tgccgtccac ctgcgcatcg aggggcgtct ctcgtgcact
     1201 ggagggatct ttctcgatgt ccggaaggtg ctggaatcca atgagcggaa ccaggtacgg
     1261 gcaattctct acaagtacca cgcgggaatc gaaggaccgc aacatcgccc gatctttcgc
     1321 tatgataatt cccatacgta cctccgagaa gggcatccgg atccctttca caaacaccgc
     1381 ttcgaccccg atacgtggca ggagatcaca ccgccgttgt ggatcggcta ccacgagtgg
     1441 cccacgttag ccgacgtgct cgaagaactg catgcgtggt ggttggcgat gcgagacagc
     1501 ctcgacctcc caacctgact catctcccac aacttgccgg atcttcctgg ctatcctcag
     1561 acgagatgga aactgcttgc cagctactac tcgtagctct gtaccgatcg ctcaattgtt
     1621 aggctggcgc gcgggctgcg ggtggcgtag cgttgtggcg gtgagactag ccggcggctg
     1681 aagtgcggga gagggagcat gacgaccaga gagcagaccg agcagtttcg ggtcgagcgc
     1741 gacagcatgg gcgagatgca ggtgccggcg ggcgcgcgct acggggcctc aaccgcccgc
     1801 gcggtggaga acttcccgat cagcgacctg cgcttcccgc ccgagttcat ccgcgcgctc
     1861 gggctgatca agtgggccgc cgccaaggtc aatcaggagc tggggctgct cggcccggag
     1921 aaggccacct tgatcgagca agcggcctac gaggtggccg agggcgcctt cgccgaccag
     1981 ttcccgatcg acatcttcca gaccggctcc ggcacctcga ccaacatgaa cgccaacgaa
     2041 gtcatcgcca accgtgccaa cgagctggcc gggcagcgcg tggtccaccc caacgacgac
     2101 gtcaacatgg gccagagcag caacgacgtg atcccgaccg ccatccacct gagcgcctcc
     2161 atcggcgtgg cccagcggct gctgccggcg atgcgccacc tggaggctac gctcgaccgc
     2221 cgcgccgagg agacggccca gatcgccaag atggggcgca cgcacctgat ggacgccatg
     2281 ccggtgatgc tggggcagga ggtgctcggc tgggcgggtc aggtgcgcca cgccatgacc
     2341 gggatcaatc acgtgctgcc cagtctggcc gagctggccc tgggcggtac cgcggtcggc
     2401 accggcatca atgcgcaccc ggagttcgcg tccggcgtgg cccggctcct ggccaagcag
     2461 accggcctct ccttccagga gagctcgaac cacttctacg cccaggccac gatggacacc
     2521 gcggtgcagc tgagcggcca gctcaaggcc tacgcggcgg gcctgctcaa attcgccaat
     2581 gatctcagat ggatgaacag cgggccgcag ggtggcctgg gcgagatcgt gctaccggcg
     2641 ctgcagcccg gctcctcgat catgccgggc aagatcaacc cggtcatctc cgaggcgacc
     2701 atgatggtct gcgcccaggt ga
//
LOCUS       DASUBI010000064         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_883795, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000064 DASUBI010000000
VERSION     DASUBI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883795"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFU72_01180"
     CDS             complement(<1..850)
                     /locus_tag="VFU72_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422128.1"
                     /translation="MVSLRPLRVCFVVPYYAEGRESVDIDRYLSETPIHRALPRELAA
                     RGHQVQVVHLFAREVEWWEGGVRHHFVRAGRGARVLGHWMGRLRGRDRAYYEPAWRAI
                     ERVRACHPDLVHVHGATLNLNLHLLLRSLEPSLPVVLHYHGGYPSRLRLVRRLQRANF
                     ERAARYCFTTQEHALPFVEAGVIARPERVVELMETSSSFQPGERARARALTEMEGDPV
                     FLWAGRLDPIKDPLTALRGFERVLCQWPDAQLYLHYLSEECLPLLREYLAGRPALRAH
                     VHPRGPA"
     gene            complement(851..1723)
                     /locus_tag="VFU72_01185"
     CDS             complement(851..1723)
                     /locus_tag="VFU72_01185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422129.1"
                     /translation="MTTEASSSAANPVGNPVGNPVGVAYDRIAPDYDQQLLGDQWMRA
                     VLWRHYLRLFHPGDQVLDIGCGTGIDAVRLAQSGMLVTAIDVSPGMIARAQERAEAAG
                     LAGLISARVLDATEIGRLTALGSGPYDGIVSAFAGLSTLPDLTRFARDAAALLRPDGH
                     LVLHLLNRFSLWECLGQLRHGRWHEARRLGSRHERIFMIGGLPVRHYLYFPLEVERRY
                     FDSGFELSRVYGLGALRPPHTLRRVPAPLAAGLGRLDERLARWPPLRGCGRFFVLDLT
                     VRSQEHDAHAPEAG"
     gene            complement(1736..>2546)
                     /locus_tag="VFU72_01190"
     CDS             complement(1736..>2546)
                     /locus_tag="VFU72_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5422130.1"
                     /translation="HSNLWALCEQIEAMGARITLLSTGLLLKRYAADVARWCSDVVVS
                     LDGSPAVHDSIRNVPRAFERLAEGVAALRAIAPNYRVTARCVVQRRNFSDLPNVIDTA
                     RRIGLDQISFLAVDVSSSAFNRPVPWGDERVDEVALSPNELAEFTRILEDVIEGHVED
                     FASGFIAESPDKLRRLGGYFAALNGQGTFPTTICNAPWVSTVIETDGTVRPCFFHAGY
                     GNIHERPLEEILNSPEARAFRRNLDVTQDPICRKCVCTLSLAPTTSVTSVD"
BASE COUNT          412 a          897 c          901 g          336 t
ORIGIN      
        1 gcgccgggcc gcgcgggtgg acgtgggcgc gcagcgccgg ccgcccggcg aggtactccc
       61 gcagcagcgg caggcactcc tcgctcaggt agtgcaggta gagctgggca tccggccact
      121 ggcagagcac gcgctcgaac ccgcgcagcg ccgtcagcgg gtccttgatc ggatccagcc
      181 gccccgccca gaggaagacc ggatcgccct ccatctcggt caacgcgcgc gcccgcgccc
      241 gctcgcccgg ctggaagctg ctggatgtct ccatcagctc gaccacgcgc tcgggccgcg
      301 cgatgacccc ggcttcgacg aagggcagcg cgtgctcctg ggtggtgaag cagtagcgcg
      361 ccgcgcgctc gaagttggcg cgctggagcc gtcgcaccag ccggagccgc gaggggtagc
      421 cgccgtggta gtgcagcacg accggcagtg acggctccag cgaccgcagc agcaggtgca
      481 ggttcagatt cagggtggcg ccatggacat gaacgagatc cgggtggcag gcgcgcaccc
      541 gttcgatggc gcgccaggcc ggttcgtagt aggcacgatc gcgcccgcgc agccgcccca
      601 tccagtgacc cagcacgcgc gccccgcgcc ccgctcgcac gaagtggtgg cgcacgccgc
      661 cctcccacca ctccacctca cgggcgaaga ggtggaccac ctgtacctgg tgtccccgcg
      721 cggccagctc gcgcggcagc gcgcgatgaa tcggcgtctc actcaggtag cgatcgatgt
      781 cgaccgactc acgcccctcg gcgtaatagg gcacgacgaa gcagacgcgc agcggccgca
      841 gcgagaccat ctatcctgcc tccggcgcgt gcgcatcgtg ttcctgggag cgcacggtca
      901 ggtcgagcac gaagaagcgg ccgcagccgc gcagcggcgg ccagcgcgcc agccgctcgt
      961 cgagccggcc cagcccggcg gcgagcggcg ccggcacgcg gcgcagggtg tgcggcggtc
     1021 gcagagcgcc caggccatag acccggctca gctcgaagcc ggagtcgaag tagcgccgct
     1081 cgacctccaa cgggaagtag aggtagtgcc ggaccggtag gccgccgatc atgaagattc
     1141 gctcatggcg gctccccagc cgccgcgcct cgtgccagcg cccgtgccgc aattggccga
     1201 gacactccca caggctgaag cggttcagca ggtggagcac cagatgccca tccggccgca
     1261 gcagcgcggc ggcgtcgcgg gcgaaacgag tcaggtccgg cagcgtgctc agcccggcga
     1321 aggccgagac gatgccgtcg tatggcccgg agccgagcgc ggtcagccgg ccgatctcgg
     1381 tcgcatccag cacgcgggcc gagatcaggc ccgccagccc cgcggcctcg gcccgctcct
     1441 gggcgcgcgc gatcattccc ggcgagacgt cgatcgcggt caccagcatg ccggactgcg
     1501 ccaggcgcac ggcgtcgatg ccggtgccgc agccgatatc gagcacctgg tcgccggggt
     1561 gaaagagacg caggtaatgg cgccagagaa cggcgcgcat ccactggtcg cccagcagtt
     1621 gctggtcgta gtcgggcgcg atccggtcgt aggccacgcc tacagggttg cccaccgggt
     1681 tgcccaccgg gttggccgcg gagctcgacg cctccgtcgt cacggccctc ccttctcagt
     1741 ccacgctcgt taccgatgtc gtgggcgcca gcgacagggt gcagacgcac ttgcgacaga
     1801 tcggatcctg ggtgacgtcg aggttgcggc gaaaggcgcg cgcctccggc gagttgagaa
     1861 tctcctccag cgggcgctcg tggatgttcc catagccggc gtggaagaag caagggcgca
     1921 ccgtgccgtc ggtctcgatc acagtcgaga cccagggcgc gttgcagatc gtggtcggga
     1981 acgtgccctg gccgttcaac gcggcgaagt atccgcccag gcggcgcagc ttgtcggggc
     2041 tctcggcgat gaaaccggag gcgaagtctt cgacatggcc ctcgatcacg tcctcgagaa
     2101 tccgagtgaa ttcggccagc tcgttgggac tgagcgctac ctcgtccacc cgctcgtcgc
     2161 cccaggggac cgggcggttg aaggcgctgg aggagacgtc gaccgccagg aacgagatct
     2221 ggtcgagccc gatccggcgc gcggtatcga tgacattcgg caggtcgctg aaattgcgcc
     2281 gctgcaccac gcagcgcgcc gtgacgcggt agttcggcgc gatcgcccgc agcgctgcca
     2341 cgccctcggc cagccgctcg aaggcacgcg gcacgttgcg gatcgagtcg tggaccgccg
     2401 ggctgccgtc gagcgagacg accacgtcgc tgcaccagcg cgccacgtcg gcggcgtagc
     2461 gcttgagcaa gagcccggtc gagaggagcg tgatacgggc acccatcgcc tcgatctgct
     2521 cgcagagcgc ccagagattc gagtgc
//
LOCUS       DASUBI010000065         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_65858, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000065 DASUBI010000000
VERSION     DASUBI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65858"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFU72_01195"
     CDS             complement(<1..507)
                     /locus_tag="VFU72_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422131.1"
                     /translation="MPGQIRVVTDGMGLARDSAYRGIGTYCRHVLAALAEEADLEVTA
                     LVPTGTEVPDGVRARRVLRVAPGRWAAEEHDLLLPLDLLRTPGDVLFSPGKLPPRWSP
                     YPVVQTVHDLVPLSDPTASPSERREWQRASRRWQHAAAVVAVSAHTAVEATATLGLDS
                     SRLHVVPHG"
     gene            complement(511..1980)
                     /locus_tag="VFU72_01200"
     CDS             complement(511..1980)
                     /locus_tag="VFU72_01200"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU5422132.1"
                     /translation="MDLVGYLGVLRRRWLAILLCVIAGICGGYYTGHHGVKTYQATAR
                     VIVNVPAAANVQDQLAGSQLSSNLVQTYATMATSQVVAERVVRDLGLSESPAAIASAL
                     SAAPVPNTYIIEISASDINPQQAQALANSAANSLRDAVASFDSNVSNRVTIQVMDLAA
                     LPTVPINPRPRLDLIVGGILGVVAGLLIAALLEALDRTMKSVQQGEALIQVPLIGLVP
                     RRRGSPLAVATGGAPEGEPYRAMRTAIRFLEPDKPLRTLLVTSPMPGDGKTTTAANLA
                     LALSQSGERVIVVDADLRRAQLAEMFGLERAVGLTSMVLGTAKLSETLQPWNEQLDLL
                     ASGPLPPNPSEILVSQLFHTILQELHDLADIVIIDAPPVLPVTDAVALAAQVDGVVMV
                     VRHGSTLRQAAMEARRRLEGVGAKVVGYVFNAVPASESHDYYADYRYDYDSDKPRDPS
                     TLPTISEPRPAEQSATTRQIPGRPEVGTAAAQATHPSHR"
     gene            complement(1990..>2523)
                     /locus_tag="VFU72_01205"
     CDS             complement(1990..>2523)
                     /locus_tag="VFU72_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422133.1"
                     /translation="PAVQDRLRALGLADPTHYFNSGVLLMDLDALRERSSTSALLALV
                     REQADRLLWVDQDALNLVHASEWAELAPRWNVQNSFWAWPAWAAATFGQGELAAARSD
                     PAVLHFEGPSLAKPWHYLCRHPQTAAYRRAAGRTPWGWPTPVDRTWATRLIKSLPSSW
                     QTPAYLRLLRARQRVRG"
BASE COUNT          411 a          803 c          874 g          435 t
ORIGIN      
        1 gccatggggt acgacgtgca gacgagatga atcaaggccg agggttgccg ttgcctcgac
       61 cgcggtgtgc gcgctgaccg ccacgaccgc tgcggcgtgt tgccaacgcc gcgaagcgcg
      121 ttgccattct cgccgctcgg atgggctagc cgttgggtcg ctgaggggca cgaggtcatg
      181 gaccgtttgg acaacggggt aaggagacca gcgtggcgga agcttgcctg ggctgaacag
      241 cacgtcgccg ggcgtgcgaa gcaggtccag cggcagcaaa agatcgtgct cctccgccgc
      301 ccagcgacct ggggcgacac gcagaacccg ccgcgcccga acaccgtcgg ggacctcggt
      361 cccggtcggc accagtgccg tcacctcaag gtctgcctcc tccgccaagg cagcaaggac
      421 gtggcggcag taggtgccga tgcctcggta ggcggagtcg cgcgccagtc ccatcccgtc
      481 cgtgacgact ctgatctgac caggcatggg ttagcgatgg gaggggtgcg tcgcctgggc
      541 cgccgccgta cccacttcgg gtcgccccgg tatctgccgg gtggttgccg actgttcggc
      601 gggtcgtggc tcgctgatgg tgggcaacgt cgacggatct cggggcttgt cggagtcgta
      661 gtcgtagcgg tagtcggcgt agtagtcgtg gctctcgctg gcgggcacgg cgttgaagac
      721 gtagccgaca accttcgctc cgactccttc caaacgccgc cgcgcctcca tcgcggcctg
      781 gcgcaacgta ctgccgtggc gcacgaccat gaccacgccg tccacctggg cagccaaggc
      841 gactgcgtcg gtgaccggca gcaccggcgg agcatcgatg atcacgatgt ccgcgagatc
      901 gtggagttct tgaagaattg tgtggaacag ctgcgatacc agtatctcgg aagggttggg
      961 cggcagcggg cccgaggcga ggaggtcgag ctgctcgttc cacggttgga gagtttcgct
     1021 gagcttcgcc gtcccgagca ccatcgacgt gagtcctaca gcgcgttcaa ggccgaacat
     1081 ctcggccagc tgcgcgcgac gtagatctgc gtcgacgacg atgacgcgct cacccgactg
     1141 ggacagcgct agcgccaggt ttgccgccgt cgtggtcttc ccgtcccctg gcatggggct
     1201 ggtcaccagc agagtgcgca gcggcttatc cggttccagg aatcggatcg cagtcctcat
     1261 cgctcggtaa ggttcgccct ccggcgcgcc tccggtggcc actgcgagag gcgatcctcg
     1321 gcgccgcggc acgagtccga tcaacggcac ttggatcagt gcttcccctt gctgcaccga
     1381 cttcatggtg cggtccagcg cttcaaggag cgccgcaatg agcaatccgg ccaccacgcc
     1441 gaggatgccg ccaacgatga ggtcgagcct ggggcgcggg ttgatcggta ccgtcggaag
     1501 ggccgccaga tccatcacct ggatcgtcac ccggttactc acgttgctgt cgaagctcgc
     1561 gactgcgtcg cgcagggagt tggcggcgga gttggctagc gcttgtgcct gctgggggtt
     1621 gatgtcggag gccgatatct cgatgatgta agtgttcggc acgggggccg ctgatagtgc
     1681 gctcgcgatc gcggcgggac tctcggacag gcccaggtct cgaactactc gctcggcgac
     1741 aacctgcgat gtcgccatcg tcgcgtaggt ctggaccaga ttgctggaca gctgggaacc
     1801 ggccagctga tcctggacgt tcgccgccgc cgggacgttg acgatcaccc gggccgtcgc
     1861 ttggtaggtc ttcacgccgt ggtgccccgt gtagtagccg ccgcaaatac cggcgattac
     1921 gcagaggagg atggccaacc atcggcgccg taggacgccg aggtagccga caaggtccac
     1981 gaggagaggt tagccgcgaa cgcgttgccg cgctcgtagc agccgcaggt acgcaggcgt
     2041 ctgccaggag gatggcaggc tcttgatcag tcgcgttgcc cacgtgcgat caacgggtgt
     2101 cggccacccc cacggcgtcc tcccagcggc tcttcggtat gcggcggttt gcggatggcg
     2161 gcagaggtag tgccaaggct ttgccagcga cgggccttcg aagtgcagca cggctgggtc
     2221 gcttcgggcc gccgcaagct ctccctgtcc gaaggtcgcg gccgcccagg cgggccatgc
     2281 ccagaagctg ttctgcacgt tccagcgggg cgctagctca gcccactcgc tcgcatgcac
     2341 gaggttgagc gcgtcctggt ccacccacag caggcgatca gcctgctccc tcactagggc
     2401 aagcagcgcg gatgtggagg agcgttcgcg cagtgcgtca agatccatga gcagcacacc
     2461 cgagttgaag tagtgcgtcg ggtcggccaa ccctaacgca cgcagccggt cctgcacggc
     2521 tgg
//
LOCUS       DASUBI010000066         6119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1319508, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000066 DASUBI010000000
VERSION     DASUBI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319508"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VFU72_01210"
     CDS             <1..1475
                     /locus_tag="VFU72_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5422134.1"
                     /translation="AILAAEVGRWRAQDERLDARSGRVLARLRREAQALGNAPLDELL
                     PPLERAAHGLADRLGKQVTLQVEFQGATLPRAALGRLGDALLHLVRNAVDYGIEPPAQ
                     RRARGKPETGTLVLRARQDAGALLLEVSDDGAGIDDAQIVQRAIESGFPIPPEGLSRE
                     RVLQLLLLPGLTTRPAGARPSAGQGLAVVGTIAAELKGTVQIASTLGAGTTIRLLLPG
                     TLSSLPAVIVALGAARYALPFVQAEPVPRPLVRQSEETGDETGFVAELGGLRFPAADL
                     GALLGLRSTWSVDEEGGAWLRVRQESEEQLIRVDALIGRRELALQPLDAGALPYPPGV
                     VARAELEPGDIALVVDLAQALGGRGAPAVASGRESPVALVADDSIAARRAIGRVLERH
                     GWRTLEARDGLEAWEMLGSVAPALLVVDLDLALLDAAQVIRAAQDQHGIPVIALAGRA
                     GAANQHQLHALGIAAVLDKPIDGDDLVAALGYLAPQAPRR"
     gene            complement(1538..2671)
                     /locus_tag="VFU72_01215"
     CDS             complement(1538..2671)
                     /locus_tag="VFU72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HEU5422135.1"
                     /translation="MSEAQARATGLVFNDRYLQHNTNPYRLRSGAPLPFVEAVDHYSN
                     PRLVERTKKLLDLTGLSAKLTPIAAYPATVEDLAAYHDLDYVRRVEALCRAGGGETGQ
                     GAPAGADSYEIARLAAGGVMAAVDAVMQGEVAQCFALVRPPGHHAMADKGMGFCIFSN
                     VAVAAHYARRRYGLERILILDWDVHHGNGTQDAFYRDPGVLFVSLHQDGLYPPGFGER
                     EQTGAGPGEGHTVNIPLPAGSGDAAYLAALERVVLPIARQFQPQLVMISAGQDASAND
                     PLGRMCLSAEAYRRMTALLGQFAGETAGGRLVVAQEGGYSEIYGPYCTLAIAEELAGE
                     RTGIEEPMAAERIAAWPSSRTVSPDQAAAIEAAAETQRRYRPL"
     gene            complement(2694..3734)
                     /locus_tag="VFU72_01220"
     CDS             complement(2694..3734)
                     /locus_tag="VFU72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU5422136.1"
                     /translation="MASTPDDEELRGSEAYYHVVLERIPPRAEPAFETAAMQERVWGR
                     RWGVHDDVGRLRLCAVHRPGAEIGVIDPAKYDPGIAALIDDAQQWYWRDRRGPDLALM
                     QAQHDALVAALQAEDIEVVQVESSAGDPKAVFTRDQAIAVHGGAVICRMGPVGQERGY
                     GRRGEEAYVTRVLAALGMPILRTIHGTGLLEGGSFLFLNETTAALGLSFRQNETGARQ
                     IEQVLAEQGVRLIRVSLTGHSLHLDGCIVMVDRDKAIINMARLPYWFLDTLKELGIQP
                     IHVYPNEEGNKPVNCLALRPGRVLIAAGAPRTVERLNQAGVETVEIPYGEIHKNGGGI
                     HCSTLPLVRDPS"
     gene            3985..5664
                     /locus_tag="VFU72_01225"
     CDS             3985..5664
                     /locus_tag="VFU72_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422137.1"
                     /translation="MTIDTARVEELISGGETLTVEFKGEKQRQLSDAEICEAIVCLAN
                     SDGGVLLVGVENDGTVTGVRSRHQGTIDPRLMQIALFNKTMPSLDTHASVHTVAGKHV
                     LAIEVDPCPDVCSTMDGRCIHRVDGVHGPECRPFFPHQHAARRADLRRLDYSAHRIDG
                     ANWNDLDPLEIERLRQTISRLNGDRLLLSLDDHQLTQALQLVETRVTELVPNVAGLLL
                     LGREEALRRLVPTHEITFQALGERGEVLINDWFHSPILKVLEAVEQRFSARNLEQEVT
                     VGLFRLPIPDYAPDAFREAVNNAVLHRDYARLGTVFVQFHPDHLLITNPGGFLEGITL
                     DNLLVHEPKPRNPRLAEAFRRIGLVETTGRGIDRIYYGQLIYGRPLPEYGTSDRDTVR
                     VILRGGAASLAFSRLVYEQSRTGEPLSLVELLILNHLQHERRADVGSIARLTQRDEAY
                     VRRALERLVERGFAEARGERRARVYHLSASLYRELGNSAEYVRAHGFDAIRQEAMILE
                     YVAAHGEIRRSEVVELCGLTERQASYLLRRLVSAGRLRQEGSRRWTFYVPV"
     gene            5937..>6119
                     /locus_tag="VFU72_01230"
     CDS             5937..>6119
                     /locus_tag="VFU72_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422138.1"
                     /translation="MARLPDVPDEAMAPELAPLVDAQLKEYGRTLNSLRQSAHTPPIA
                     LASPAMGRAISRSGQIA"
BASE COUNT          917 a         2048 c         2115 g         1039 t
ORIGIN      
        1 cggccatcct ggccgccgaa gtgggccgct ggcgcgcgca ggatgagcgg ctcgacgcgc
       61 gcagcggccg cgtgctggcg cgcctgcgcc gtgaggccca ggcgctgggc aacgccccgc
      121 tggacgagct gctgccgccg ctcgagcggg cggcgcacgg gctggcggac cggctcggca
      181 agcaggtcac cctccaggtt gagttccagg gcgccaccct gccgcgcgcc gcgctgggcc
      241 gcctcggcga cgcgctcctc catctggtgc gcaacgccgt cgactacggc atcgagccgc
      301 cggctcagcg gcgcgcccgc ggcaagccgg agaccggcac gctcgtgctg cgcgcccggc
      361 aggacgccgg tgcgctgctg ctggaggtca gcgacgacgg cgcgggcatc gacgacgcgc
      421 agatcgtgca gcgtgccatc gagagcggct tccccatccc ccccgagggg ctcagccgcg
      481 agcgggtgct gcaactgctg cttctccccg gtctgacgac gcgtccggcc ggcgcccgcc
      541 cctccgccgg ccagggtctg gcggtagtcg gcaccatcgc cgccgagttg aagggcacgg
      601 tgcagatcgc ctcgacgctg ggggcaggga ccaccatacg gctgctcttg ccgggaacgc
      661 tcagtagcct gccggcggtc atagtcgcgc tcggcgcggc gcgctacgcg ttgcccttcg
      721 tccaggccga gccggtgccg cgcccgctgg tacggcagag cgaggagacc ggcgacgaga
      781 ccggcttcgt ggcggagctg gggggcttgc gcttcccggc ggcggacctc ggcgcgctcc
      841 tcggccttcg ctcgacctgg tcggtcgacg aggagggcgg cgcctggctg cgcgtgcgcc
      901 aggagagcga ggagcagttg attcgggtcg atgccctcat tggccgccgg gagctggcgc
      961 tccagccgct cgacgccggc gccctcccct atccgccggg cgtggtggcg cgtgccgagc
     1021 tggagccggg cgatatagcg ttggtggtcg atctggcgca ggcactcggg ggtcgcggtg
     1081 cgccagccgt ggcgtcggga cgggagagcc ccgtcgcgct ggtggccgac gactcgatcg
     1141 cggcgcgacg cgccatcggg cgcgtgctcg agcgccacgg ctggcgcacg ctcgaggcgc
     1201 gcgacggtct ggaagcatgg gagatgctcg gcagcgtggc gccggcgctc ctggtggtcg
     1261 atctcgacct cgccctgctc gacgcggccc aggtcatccg cgccgcgcag gaccagcacg
     1321 gcatcccggt catcgcgctg gccgggcgcg ccggggccgc caaccagcat cagttgcacg
     1381 cgctggggat cgccgcggtt ctggacaagc cgatcgacgg ggacgacctg gtcgcggcgc
     1441 tcggctacct cgccccgcag gcgccgcggc ggtagggcgc actccaaaga gccgggcgac
     1501 tgctggtcgt tactgggtga tcgtcgacgt cgggctctta cagcggccgg tagcggcgct
     1561 gagtctcggc ggcggcctcg atcgcggcgg cctggtcggg cgagaccgtg cgcgagctgg
     1621 gccaggcggc gatgcgctcc gccgccatcg gctcctcgat gccggtgcgc tctccggcca
     1681 gctcctcggc gatcgccagg gtgcaatagg gcccgtagat ctccgaatag ccgccctcct
     1741 gggcgaccac cagccgcccg ccggccgtct cgccggcgaa ttgccccaag agcgccgtca
     1801 tccgccggta ggcctcggcc gagaggcaca tgcggcccag cggatcgttg gcgctggcgt
     1861 cttgccccgc cgagatcatg accagctgcg gctggaactg ccgcgcgatc ggcagcacga
     1921 cccgctccag cgcggccagg taggcggcgt cgcccgagcc ggccggcagc gggatgttga
     1981 ccgtgtggcc ctcgcccggc ccggcgccgg tctgctcccg ctcgccgaag cctggcgggt
     2041 agaggccatc ttgatgcagc gagacgaaga ggacgccggg gtcgcgatag aaggcgtcct
     2101 gggtgccgtt gccgtggtgc acgtcccagt cgaggatcag gatgcgctcc agaccgtagc
     2161 gccgccgcgc gtagtgcgcc gccaccgcca cgttgctgaa gatgcagaag cccatccctt
     2221 tgtcggccat ggcgtggtgg cccggcgggc gtaccagcgc gaagcactgc gccacctccc
     2281 cctgcatgac cgcgtcgacc gccgccatca cgccgccggc cgccagccgc gcgatctcgt
     2341 acgagtccgc gccggccggc gcgccctgcc cggtctcgcc gccgccggcc cggcagagcg
     2401 cctcgacccg gcgcacgtag tcgaggtcgt ggtaggcggc gagatcttcg accgtggccg
     2461 ggtaggcggc gatcggcgtc agcttcgcgc tcaggccggt caggtcgagc agcttcttgg
     2521 tgcgctcgac caggcgcggg ttggagtagt ggtcgaccgc ctcgacgaag gggagcggcg
     2581 ccccggagcg cagccggtag ggattggtgt tgtgctgcag gtaacggtcg ttgaagacca
     2641 gtccggtcgc gcgcgcctgc gcttcgctca tcgcattgac tccgcttcca gtcttacgaa
     2701 gggtcacgca ccagcggcag cgtcgagcag tggatgccgc cgccgttctt gtggatctcg
     2761 ccgtagggga tctcgaccgt ctcgacgccg gcctggttca gtcgctcgac ggtgcgcggg
     2821 gcgccggcgg cgatcagcac gcggccgggc cgcagcgcca ggcaattgac cggcttgttc
     2881 ccctcctcgt tcggatagac gtggatcggc tggatgccca gctccttgag cgtgtcgagg
     2941 aaccagtagg gcaggcgggc catattgatg atcgccttgt cgcggtcgac catcacgatg
     3001 cagccgtcga ggtggagcga gtggccggtg agcgacacgc ggatcaggcg cacgccctgc
     3061 tcggccagca cctgctcgat ctgccgcgcg cccgtctcgt tctgccgaaa ggagagcccg
     3121 agcgcggccg tagtctcgtt caagaagaga aacgacccgc cctccagcag cccggtgccg
     3181 tggatggtgc gcaggatcgg catgccgagc gcggccagca cgcgggtgac gtaggcctcc
     3241 tccccgcgcc gcccgtagcc ccgctcctgg cccaccggcc ccatgcggca gatcaccgcg
     3301 ccgccgtgga ccgcgatcgc ctggtcgcgg gtgaagacgg ccttggggtc gccggcggag
     3361 ctctcaacct ggacgacttc gatgtcctcg gcttgcagcg cggcgactag cgcgtcgtgc
     3421 tgcgcctgca tcagcgccag gtccggcccg cgccggtcgc gccagtacca ctgctgcgcg
     3481 tcgtcgatca gggcggcgat gccggggtcg tacttggccg ggtcgatcac gccgatctcg
     3541 gcgccggggc ggtggaccgc gcacagccgc agccgcccga cgtcgtcgtg cactccccag
     3601 cggcggcccc agacgcgctc ctgcatcgcg gccgtctcga aggccggctc ggcgcgcggc
     3661 gggatgcgct ccagcaccac gtggtagtag gcctcgctgc cgcgcagctc ctcgtcgtcg
     3721 ggcgtgctcg ccatcgtcat cctctcctcg ctggtatgta tcgccgcgat tgtcgcacgc
     3781 gccgccgggg tggtgccagc ggttgccggc gccttcttgg actgctatgg ttcacactgc
     3841 cctgtcaccg tcgattccta ggagattttc gcgacatacg cttgctccgt cggagagtcg
     3901 tcaaaccgcg tcaacattgg cgttattaga tcccctatcg agcagggcga gttgagttcc
     3961 gacaattaca gaggctagtt gttgatgaca attgacaccg cgcgggttga agagctgatt
     4021 tccggcggcg agacactcac ggtcgagttc aaaggcgaaa aacagcggca gctctccgat
     4081 gccgagatct gcgaggcgat cgtctgcctc gccaactccg atggcggcgt cttactcgta
     4141 ggtgtcgaaa atgacggaac tgtaacgggc gtcaggtcgc gccaccaagg cacgatcgat
     4201 cctcggctca tgcagattgc cctcttcaac aagaccatgc cgtctctcga cacacatgcg
     4261 agtgttcaca cggtagcggg taagcacgtt ctggcaatcg aggtcgaccc ttgtcctgac
     4321 gtatgctcga cgatggatgg gcgttgtatt catcgggtcg acggtgttca cggaccggaa
     4381 tgccgtcctt tcttcccgca tcagcacgcg gccaggcgtg ccgacctgcg gcgtctggac
     4441 tactccgctc accgaatcga tggtgcgaac tggaatgact tggacccgct ggagatcgag
     4501 cgcctccgac agacgatcag ccggctcaac ggcgatcgcc tgctattgtc gctcgatgac
     4561 catcaattga cccaagcact ccaactcgtc gaaacgcgtg tgaccgaatt ggtcccgaat
     4621 gtggccggtc tcttgctgct ggggcgagag gaggcgctgc ggcggctcgt ccctacgcac
     4681 gagatcacct tccaggcatt gggcgagcgc ggggaggtgc tgatcaacga ctggtttcac
     4741 tcgccgatcc tcaaagtgct tgaggcagtt gaacagcgct tcagcgcgcg aaacctggag
     4801 caagaggtca ccgtcggcct gttccggctt ccgattcccg actacgctcc tgatgcgttt
     4861 agagaagcag tcaacaatgc cgtattgcat cgtgattacg cccggctggg gaccgtcttt
     4921 gttcagtttc acccggatca tctgttgatt acgaatcccg gcggctttct ggaagggatc
     4981 acgctcgata atctcctggt ccacgagcca aagccgcgca acccgcgcct tgcggaggcg
     5041 tttcgtcgga ttggcttggt ggagacgaca ggtcggggta tcgaccgcat ctactacggc
     5101 cagctcattt acggccgccc gcttccagag tatggtacga gcgatcggga taccgttcgc
     5161 gtaattcttc gtggcggtgc ggcgagtctg gcattctcaa ggctcgtgta tgagcagagc
     5221 cgaaccggcg agccactgtc attggtcgag ttgctgatac tcaaccacct gcagcatgag
     5281 cgacgggctg acgtgggctc gatcgcccgg ctgacgcagc gcgacgaggc gtacgtccgg
     5341 agagctctgg aacgcctcgt cgaacgtgga tttgccgagg cgaggggcga gcggcgggct
     5401 cgcgtgtatc acctgagtgc gtcgttgtat cgagagcttg gcaattccgc tgaatacgtt
     5461 cgcgcgcacg ggtttgacgc catccgccag gaggccatga tccttgagta cgtagccgcg
     5521 catggagaga ttcggcgctc cgaggtcgtt gagctgtgcg gcttgaccga gcggcaggct
     5581 agctatctgc tgcggaggct tgtgagtgcg ggccggctgc gccaagaagg gtcacgacgc
     5641 tggacgtttt acgtcccggt ttgatacggc catgtgccat cctcgtataa acactcgata
     5701 cttagggtgc tcgtcggtgt gtcgggcggt gatcgacgac aatccgtgta cgaataccag
     5761 ttccgagggc agtctcagca gtgttggttt caggagacac atacctatga acactcagac
     5821 gcgtgctatg aacaatggtc atagccgtat gaacaacgtg cataccagcg cctcggtacg
     5881 ctcaaactct tcgcacggca actgttttgg gtccataccc acgataggag gtatcaatgg
     5941 cccggcttcc cgatgttccc gatgaggcga tggcgccgga gctggcgccg ctggtggatg
     6001 cgcagctgaa ggagtacggc cggacgctga actcgctgcg ccagtctgcc cacaccccgc
     6061 cgatcgcgct ggcctcgccg gcgatgggcc gcgccatctc ccgctccggc cagatcgcg
//
LOCUS       DASUBI010000067         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1074560, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000067 DASUBI010000000
VERSION     DASUBI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074560"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="VFU72_01235"
     CDS             complement(<1..1572)
                     /locus_tag="VFU72_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEU5422139.1"
                     /translation="MAIEERDTRPGTELVAVGETAEGYVAALAAQGVDCLFLNPGTDT
                     FPIQEALAKLAALGRPAPRTVLCLFEVVALAAAHGYYAATGRPQAVLVHVDVGSQNLG
                     SMLHDAQRGRAAVVIAAGRAPYTTDPSQRGSRSSYIHWLQEQLDQHGIVRNYVKWDYE
                     LRRPDQVGEVVARAFQIAASEPPGPTYLTLPREVLMSSPGEVAIAQPGRFPPVGLGAG
                     DPDALREVARRLVAAERPLVLTANTGRSVAGFDGLLRLAETLALPVVEWRNRANFPAD
                     NPLHQGYQARPLLESADLVLALDHDFPYIPTLDQPAPSAQIIQIDVDPVKERIPLWSF
                     PVDLPIRADTGKALDLIADFALAQVDDEARQRIERRRAELTARHQRQRSDWEAAAYND
                     REARPLTPTWLSYCVGRLADEHPECVFVDETVTNNITTWQQVPAREPGSWFVSGGSGL
                     GWGLGGALGVKLARPDRPVLALVGDGSFVFGAPLAALWAMQLQQAPVLIVIYNNTCYN
                     ATKQPLVANYPEGYSV"
     gene            1720..>2078
                     /locus_tag="VFU72_01240"
     CDS             1720..>2078
                     /locus_tag="VFU72_01240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5422140.1"
                     /translation="MSHFTPAEISYLESQRLGRLATLGRSGELHVVPVRFHYNAQLDT
                     IDVTGRFLGQSKKYRDVQDDARAAFVVDDVAGPGQPRGVEVRGQAEAVPTGGDAITPG
                     ADPEFIRITPTRIHSWG"
BASE COUNT          285 a          729 c          768 g          296 t
ORIGIN      
        1 gacggagtag ccctcggggt agttggccac caggggctgc ttggtcgcgt tgtagcaggt
       61 gttgttgtag atcacgatca gcaccggcgc ctgctgcagc tgcatggccc agagcgcggc
      121 cagcggtgcg ccgaagacga aggagccgtc acccaccagg gccagcaccg ggcggtcggg
      181 ccgcgccagc ttgaccccca gcgcgcctcc cagcccccaa cccagccccg agccgccgct
      241 gacgaaccag ctccccggct cgcgcgccgg cacctgctgc caggtggtga tgttgttggt
      301 caccgtctcg tcgacgaaga cgcactcggg atgctcgtcg gccagccggc cgacgcagta
      361 gctcaaccag gtgggagtca gcggccgcgc ctcgcggtcg ttgtaggcgg cggcttccca
      421 gtcggaccgt tgacgctggt ggcgcgcggt caactcggcg cggcggcgct cgatgcgctg
      481 ccgggcctcg tcatcaacct gagccagcgc gaagtcggcg atcaggtcga gcgctttgcc
      541 ggtgtcggcg cggattggca ggtcgaccgg gaagctccag agcggaatgc gctccttcac
      601 cgggtcgacg tcgatctgga tgatctgggc gctgggcgcc ggttgatcga gcgtcggaat
      661 gtaggggaag tcgtggtcga gcgccagcac caggtcggcc gactccagca gcggccgggc
      721 ctggtagccc tggtgcaacg ggttgtcggc ggggaagttg gcgcggttgc gccactcgac
      781 caccggcagc gccagcgtct ccgccagccg cagcaggccg tcgaagccgg cgacgctccg
      841 tccggtgttg gcggtcagca ccagcgggcg ctcggccgcg accagccggc gcgccacctc
      901 gcgcagcgcg tcggggtcgc cggcgcccag cccgaccggc gggaagcgcc cgggctgggc
      961 gatggccacc tcgccgggac tgctcatcag cacctcgcgc ggcagcgtca ggtaggtcgg
     1021 gccgggcggc tcgctggcgg cgatctggaa ggcgcgcgcg accacctcgc ccacctggtc
     1081 cggccgccgc agctcgtagt cccacttcac gtagttgcgc acgatgccgt gctggtcgag
     1141 ctgctcctgc agccagtgga tgtagctgct gcggctgccg cgctggctgg ggtcggtcgt
     1201 gtagggcgcg cgcccggccg cgatgacgac ggcggcgcgg ccgcgctggg cgtcgtgcag
     1261 catgctcccc aggttctgcg agccgacgtc gacgtgcacc agcaccgcct ggggtcggcc
     1321 ggtggcggcg tagtagccgt gcgcggccgc cagcgccacc acctcgaaga ggcagaggac
     1381 ggtgcgcggg gccggccgcc ccagcgccgc cagcttggcc agcgcctcct ggatcgggaa
     1441 ggtgtcggtg ccggggttga ggaagagaca gtcgaccccc tgcgccgcca gcgccgcgac
     1501 gtagccctcg gcagtctccc ccacggccac cagctcggtg ccgggccggg tgtcacgctc
     1561 ctcgatcgcc atctcgccct cttccctccg catgtgcccg cgacgagtcc attatagggg
     1621 cgaaacgctt tgaacggatg caagggttcg agggtgtttc gccattgggc cgccgcgcgt
     1681 agactccgtc gcgtgggttg gatcagtcga ggaggacgcg tgagccactt tacccccgcc
     1741 gagatcagct acctggagag ccaacggctg ggccgcctgg cgacgctcgg ccgcagcggc
     1801 gagctgcacg tggtgccggt gcgcttccac tacaacgccc agctcgacac catcgacgtc
     1861 accgggcgct ttctcgggca gagcaagaag taccgcgacg tgcaggacga cgcgcgggcg
     1921 gccttcgtgg tggacgacgt ggccgggccg gggcagccgc gcggggtcga ggtgcgcggc
     1981 caggccgagg cggtgcccac gggcggcgac gcgatcacgc cgggggccga cccggagttc
     2041 atccgcatca ccccgacgcg catccactcc tggggcat
//
LOCUS       DASUBI010000068         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_679239, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000068 DASUBI010000000
VERSION     DASUBI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679239"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFU72_01245"
     CDS             <1..275
                     /locus_tag="VFU72_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422141.1"
                     /translation="HRFSEIVGSDRGVRSLWFRQSGHVSEFWLVTDPLDLDHELQLQR
                     LSQALYEWNPNALIDFHVVNLRFFPGGEDDAMMVIPAAADAIQFPR"
     gene            complement(1025..1960)
                     /locus_tag="VFU72_01250"
     CDS             complement(1025..1960)
                     /locus_tag="VFU72_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5422142.1"
                     /translation="MGHIIVPLDGSGHNEAALPVAESLARTLGAPIELVQVVPGRGTR
                     DRDDEKPARLYLDSIARALPPELRAEITICSGDPAQCLLELAEARALEPPALMVMGTH
                     GRSGIGRAVIGSVADRLARSAPIPVTLVREPGQRTGPLQCLLVPLDGSALAEQALPVA
                     LELARESGATVHLVRVVEPYWRSLAAAMGVATAYVPPSDSAEVEQDMIDEARAYLDGI
                     ARQQRRVGARVAWEVRTGPAAPEILRAASTIGADLVIVSSHGRGGLQRLALGSVADVL
                     VRTGPAPVMLVPRAAQGTGELSERRLERPRRAVAS"
     gene            2207..2911
                     /locus_tag="VFU72_01255"
     CDS             2207..2911
                     /locus_tag="VFU72_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422143.1"
                     /translation="MVRKQLILVVDDDTSILRLVRIKLQAEGYQVITATNGQEAVELV
                     EREHPDLVVLDLMMPVMDGMEALRQIRRLSRVPVILLTARSSGADKVRGLDLGADDYV
                     TKPFDPDELAARVSAVLRRTHGVAVPAAQVLQYPEATIDLENRRVTVGGQEIALSRTE
                     WELLAQLAGNAGRVMPHGELLARIWGPEFRDETYYLRTWVSRLRKKLQSDRDGSVQIK
                     TFPGIGYRLEPPAEAS"
BASE COUNT          495 a         1034 c          966 g          472 t
ORIGIN      
        1 cccacaggtt ttctgagatc gtcggaagcg accgtggagt ccgcagcttg tggttccgac
       61 aatccggcca cgtttctgaa ttctggctcg tcacggatcc acttgacctt gaccacgaac
      121 tccaactcca gcggctcagc caggctctgt acgagtggaa tccgaacgct ctcattgact
      181 ttcacgtcgt caatctgcgc ttctttcccg gcggtgagga tgacgccatg atggtgattc
      241 ccgccgccgc cgacgcaatt caattccccc gctgagttgt ggagtacaaa acccagttac
      301 atattgccgc tgctgaacga aaccatgcgc ttgcgagcga aatcctcttt aacccgccgc
      361 cgctatcacc ggagcccttt gagtgggtcg tcgtcgtcgc gttttacagc acgctccatt
      421 atgccaacgc ctacttgtgg gagcgacacc gattcgagac caataggcat gccgaacgcg
      481 gccaaaggtt agcccaagac tcagactggc tgccggtgga tcctgactat cgaacacttc
      541 ggagctggtc agaaacagcc cggtacacgc ctttgtcccg cttcagcggc aatgatgcgc
      601 agcaagcggt cgatctgctt tgcagcattg aggggcacgt tcgaagcctc ctgaaattgc
      661 cttcctatca accataagag gcactcggca cgctcacaac atcgagacaa gtagccagcg
      721 tgactggccg ccgcgcacga caagctcgac cgcgcggtgt tcgcggccta cggctggctg
      781 gcggacctca gcgacgagga gctgctgggg cggctgcggg agctaaatcg ggagcgggcg
      841 ggtgccagcg cgggcgacgc ggtccgcgct cgcggggacg cggattgagt ccggcatggt
      901 ctttagccgg aatggatccg cctacagcgg taggggcaga cctccgtgtc tgcctgttcc
      961 ccctaaggca tggcaacggg gacaggtgac cggcgcacgg cggccaacga cggtgcgatt
     1021 cgctctagga ggccaccgcc cgccgcggac gctccagccg gcgctcactc agctcgcccg
     1081 tgccctgtgc ggcacgcggg acaagcatga ccggcgccgg cccggtccgg accagcacgt
     1141 cggccacgct gccgagcgcc aggcgctgca gcccgccgcg cccgtggctc gaaacgatca
     1201 ccaggtcggc gccgatggtc gaggccgcac gcaggatctc gggcgccgcc ggtccggtgc
     1261 gcacctccca ggccacccgc gccccgaccc gccgctgctg ccgcgcgatg ccgtccagat
     1321 aggcgcgcgc ctcgtcgatc atatcctgct cgacctcagc gctgtcgctg gggggcacgt
     1381 aggccgtggc cacgcccatc gcggccgcca gcgagcgcca gtagggctcg accacgcgca
     1441 ccaggtgcac ggtggcgccg ctctcgcgcg ccagctcgag cgcgaccggc aacgcctgct
     1501 cggccagggc cgagccgtcc agcggtacca gcaggcactg caaggggccc gtgcgctggc
     1561 cgggctcccg caccagcgtg accgggatgg gagccgagcg cgccagccgg tcggcgacac
     1621 tcccgatcac ggcccggccg atccccgagc gtccgtgcgt gcccatgacc atcagcgccg
     1681 gcggctccag ggcgcgcgcc tcggccagct cgagcaggca ctgcgccgga tcaccggagc
     1741 agatcgtgat ctcagcgcgc agctccggcg gcaaggcgcg ggcgatcgag tcgagataga
     1801 gccgcgccgg cttctcgtcg tcacggtccc gcgtgccccg gccgggtacg acctgcacca
     1861 gctcgatcgg tgcgcccagc gtgcgcgcca gcgattcggc gaccggcagc gctgcttcat
     1921 tgtggcccga gccgtcgagc ggaacgatga tgtgtcccat acgggacctc ctgcttctct
     1981 gttacgcggg ggccgcacca cgcgggatcc gcttagtgtc cggcatcagc ctaccccggc
     2041 gagcatgtca cctacgcgcc agtgacctgc ccggccgtta caatctcgta accattcagc
     2101 gcgatcgggt gctattcggc gcatcgctcg gtagacttgc gagtgtggca ggtcgcggaa
     2161 cgtgatcgtg gtattgaacc gctgaaccgg ccgaaagggt gaaaacgtgg tccgcaagca
     2221 acttatactc gtcgtcgacg atgacacctc gatcctgcgc ctggtgcgca tcaagctgca
     2281 ggcagagggc tatcaagtca tcaccgcgac caacggccag gaggcggtcg agctggtcga
     2341 gcgcgagcat cccgacctgg tggtgctcga cctgatgatg ccggttatgg acgggatgga
     2401 ggcgctgcgc cagatccgcc gcctctcccg ggtgccggtc atcctgctga cggcgcgctc
     2461 ctccggcgcg gacaaggtgc gcggcctgga cctcggtgcc gacgactacg tcaccaaacc
     2521 cttcgacccc gacgagctag ccgcgcgggt gagcgccgtg ctgcgccgca cccacggagt
     2581 cgcggtcccg gcggcgcagg tgctccagta ccccgaagcc acgatcgacc tggagaatcg
     2641 tcgcgtgacc gtcggcggcc aggagatcgc cctgagccgc acggagtggg agctgctggc
     2701 gcagttggcc ggcaacgccg gccgggtcat gccccacggc gagctgctgg cccgtatctg
     2761 ggggccggag ttccgcgacg agacctacta cctgcgcacc tgggtcagcc gcctgcgcaa
     2821 gaagctgcag tcggatcgcg acggctcggt gcagatcaag acgttccccg gcatcggcta
     2881 ccggctggag ccaccggcag aggcgtccta ggtgagcgac tccctcctcc cgtcgatcct
     2941 gaagacgctc ggcctcgcgc tcggcct
//
LOCUS       DASUBI010000069         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_965797, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000069 DASUBI010000000
VERSION     DASUBI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965797"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFU72_01260"
     CDS             complement(<1..616)
                     /locus_tag="VFU72_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5422144.1"
                     /translation="MAVEQGVLKAPTIAEVWAAAGRLAGRVVRTPLIRLNASEGPAEI
                     YLKLENLQPIGSFKLRGAGNLMLAAPPEQLARGVWTASAGNMAQGVAYFARQLGVACT
                     VVVPDTAPQTKIDAIERLGGRVVKVPFDVWFNIFRTHQYPGIEGVFVHAFADTQVMAG
                     NGTIGLEIVEDLPDVAAVVVPYGGGGLSCGVAATLRALKPSTRVY"
     gene            complement(633..1121)
                     /locus_tag="VFU72_01265"
     CDS             complement(633..1121)
                     /locus_tag="VFU72_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5422145.1"
                     /translation="MRVEAKLNELGLALPEPLKMPPNVVLPFPWVRVRGHRVYISGHG
                     PQNADGSLAGPLGKVGATVSPEEAKALARLTGLAILGSLKRELGDLDRVTAWLRVFGM
                     VNSAPGFNQQPAVINGCSELILELYGPEAGAHARSAVGLAELPFGIAVEIEAEVEIDG
                     QR"
     gene            complement(1207..1716)
                     /locus_tag="VFU72_01270"
     CDS             complement(1207..1716)
                     /locus_tag="VFU72_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEU5422146.1"
                     /translation="MALHRFYWLVPGQLAGCSRPGGLFPGGNRAPDEDLARLRLGDDL
                     VWLREQGIGAVLTLTERALMPAVIEEHTLEALHLPVPDMHAPHPEDFAAALEFIDRQL
                     VAGRPVAVHCLAGQGRTGTILAAYLIRGGIAADEAIERLRALCPGAIESQEQRDALDA
                     FARQRDWML"
BASE COUNT          299 a          726 c          694 g          295 t
ORIGIN      
        1 cgtagacgcg cgtgctgggc ttgagcgcgc gcagcgtggc ggcgacgccg cagctcagcc
       61 cgccgccgcc gtagggcacc accaccgcgg cgacatcggg cagatcctcc acgatctcta
      121 gcccgatggt gccgttgccg gccatgacct gggtatcggc gaaggcgtgc acgaagacgc
      181 cttcgatccc gggatactga tgggtgcgga agatgttgaa ccagacgtcg aagggcacct
      241 tgaccacgcg cccgccgagc cgctcgatgg cgtcgatctt ggtctggggc gcggtgtcgg
      301 gcacgaccac ggtacaggcg acgccgagct ggcgggcgaa ataggccacg ccctgcgcca
      361 tgttgccggc gctggcggtc cagacgcccc gcgccagttg ctcggggggc gcggccagca
      421 tcagattacc ggcgccgcgc agcttgaacg agccgatcgg ctgcaggttc tccagcttga
      481 ggtagatctc ggccggcccc tcgctcgcgt tgagccggat cagcggggtg cgcaccaccc
      541 gtccggccag ccgcccggcc gccgcccaga cctccgcgat cgtcggcgcc ttcagcaccc
      601 cctgctccac ggccatgccc cactccccac acttaccgct gaccgtcgat ctcgacctcg
      661 gcttcgatct ccaccgcgat gccgaagggc agctcggcca gcccgaccgc cgagcgcgcg
      721 tgggcgccgg cctccggccc gtagagctcc aggatcagct cgctgcagcc gttgatcacc
      781 gccggctgct ggttgaagcc cggcgccgag ttgaccatgc cgaagacgcg cagccaggcc
      841 gtaacgcggt cgagatcgcc cagctcgcgc ttgaggctgc ccaggatcgc cagcccggtc
      901 aggcgtgcca gcgccttggc ctcctccggg gagacggtcg ccccgacctt gcccagcggc
      961 cccgccagcg agccgtcggc gttctgcggc ccgtgcccgg agatgtagac gcggtggccg
     1021 cgcacccgca cccaggggaa ggggagcacg acattgggcg gcatcttcag cggctccggc
     1081 agcgccagcc ccagctcatt cagcttcgcc tcgacgcgca tgcgctgtct ccattccggc
     1141 gaccggcgtc ctcgcgccgc agcatagcgc agccgcgtca tcctgcctac gcgttggttg
     1201 ctgccgtcac agcatccaat cgcgctggcg ggcgaaggcg tcgagggcgt cgcgctgctc
     1261 ctggctctcg atggcgcccg ggcagagggc gcgcaggcgc tcgatggcct catcggccgc
     1321 gatgccgccg cgaatcaggt aggcggccag gatggtgccg gtgcggccct gcccggccag
     1381 gcagtgcacg gccaccgggc gcccagcgac cagttgccga tcgatgaatt cgagcgcggc
     1441 ggcgaaatct tcagggtggg gtgcgtgcat gtcgggcacc ggcaggtgga gtgcttcgag
     1501 ggtgtgctcc tcgatcacgg ccggcatcag cgccctctcg gtcagggtca gcaccgcgcc
     1561 gatcccctgc tcacgcagcc aaaccaggtc gtcacccagg cgcaggcggg ccagatcctc
     1621 gtccggcgcg cgattgccgc cggggaagag gccgccgggc cgcgagcagc ccgccagttg
     1681 ccccggcacc agccaataga agcgatgcag cgccatccgc cactccgatc gctgcgagcc
     1741 agccgctcgg gccgattgtt cctgatcgcg gtcgcgcgtg cactggagaa atggccgggc
     1801 gagggctacc cgtttggctg ggcggcaccg cgttaccctc ctgtaggggc agacccctgt
     1861 gtctgccctg tctcccctac atggaggcga tgggggaata gggcaacgtc ggcgttaccg
     1921 acaacgcccg gcagtgggca gcgagggcag acacaggggt ctgccgatac gcatcaagtt
     1981 aaggatttcg cgatccagac gcgggttttg gcgt
//
LOCUS       DASUBI010000070         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_692294, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000070 DASUBI010000000
VERSION     DASUBI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692294"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(812..1129)
                     /locus_tag="VFU72_01275"
     CDS             complement(812..1129)
                     /locus_tag="VFU72_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5422147.1"
                     /translation="MDLTAGTLLVQRQLSQDGVVSEPKTATGRRTTALSPTCVAVLRE
                     HRRQQHEERLAIGPAWEHHDLIFCTRHGRALSQRNVLREFKRLLVRAGVRDVPFHALR
                     HIH"
     gene            1362..>2800
                     /locus_tag="VFU72_01280"
     CDS             1362..>2800
                     /locus_tag="VFU72_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422148.1"
                     /translation="MADYTATPHLAPVAPLVGRAREQAILREALDAALAGRGSVVLIG
                     GEAGIGKTALAESLLTDAAGQGALVLVGRCYDLAATPPYGPWAEALNRAPRVEALPPP
                     PDLSGAGAVASQATIFAAVRDALAARAAARPLALLLEDLHWADPTSLDLLRVVARGLA
                     DTPLLLLATYRADEIDRRHPLYALLPALVREARAARLDLRALDEAALHALVAARYALP
                     PPAATRLVAYLHSRAEGNPFFAGELLRALEEEQQLRRDGVAWALGDLPALAVPPLLRQ
                     VIDARLDRLGEAARELLAVAAVIGQKVPLELWATVAGAGEDALVDLTEHAAEAHLLLP
                     TLDGARFAHALIRETLYEGTLPLRRRVLHRRVAEALLADPDPDPEAVAHHLQQAGDAR
                     APAWLEQAGARAQHAFAWLTATERYESALALLEQRADDPARRGWLLFRLAMVGGYADE
                     RRSLAWLDAAVPLLAAAQDPALAAAVRFHR"
BASE COUNT          437 a         1023 c          905 g          435 t
ORIGIN      
        1 aagcgggttg acaaacgggg gtactaaccg gctgtggcgg cactgcccgc cacggccccc
       61 cggcccgcct aaccccgttc cggttcccct ttgcaacaca ccttgccaat gggcaacacc
      121 ccggcccctt tcccccattc cccgccatag ccctatgtgt tgccctactg cgtagcatca
      181 ttgcaaggcg cggcgggacg acgcaagaat tagtagtagc ggcaagcagc gcgccgccgc
      241 ccatgcaccc accttcaccg accacccaca cacccaaaca ccccaaatac acctagcagc
      301 gcgcccacac cctaccccgt gggtgggggg aagcctaccc gcttcggccc cgcccacagt
      361 gcttgacggc ataaccaaac ggccctatga tagacctgtt ggctaggggc tatccggccg
      421 acgaacctac cgcattttgc tagattctat gcggttttag gcgctagcag gctggccctg
      481 gttgcggacc aaaaggtcgc aggttcgagt cctgccaggc gtaccccgaa acaaccgcat
      541 gaacgcaaga aaccggcgcg ggcagcaggc ccgcgccgcg attgtattcc tctgttgggc
      601 gcaatttccg ccgatggcgg catttgagtg gcgcctggag ccattgagca tcaggcaggg
      661 cgcgcactgg cagcctacgc cagtaggaca tcaaaacgct ccgcagcggc gcggcccagt
      721 gttggcagat acacgctaca gatgtcgaac gtcatgctaa tgttcgcgtg cccaagccgt
      781 tcttggacaa ccttcgggtg aacgctctgc gttaatgaat gtgacgcaag gcgtggaacg
      841 gcacatcacg cacaccagca cgcaccaaca gccgcttgaa ttcccgcagc acattgcgct
      901 gactcaacgc gcgaccgtga cgcgtacaga aaatgagatc gtggtgctcc caggccggtc
      961 caatcgccag ccgctcctcg tgttgctgcc gccggtgctc ccgcaacacc gcgacacagg
     1021 tgggcgacag agcagtggtg cgtcgcccgg tggcagtctt cggttcactc accacaccgt
     1081 cctgactgag ctgacgctgc accagcagtg tgccagcagt cagatccacg tctcctcata
     1141 gaaggccgag gagtttctcg gtccgcgctt cgctggcaag ggcgaccgta atcagcgcgt
     1201 cccagcgact gcggcacgtc gccaaccgta gccgcgtgac ctcttggcgt acaacgcgac
     1261 aactccacag cgctggtttg gcaatccgca aggaggcttc gcagcggcgc atgctatact
     1321 cggaggcaca gataccagat ttccacgccg cgaggccgcc gatggccgac tacactgcca
     1381 caccgcatct ggccccggtt gcgccgctcg tcgggcgtgc gcgcgagcag gccatcctgc
     1441 gagaggcact cgatgcggcc ctagcgggac ggggctccgt agtactcatt ggcggcgagg
     1501 cgggcatcgg caagaccgcg ctcgccgaat cgctgctcac cgatgcggca ggtcagggcg
     1561 cgctggtgct tgtggggcgc tgctacgacc tggcggcaac gccgccctat ggcccttggg
     1621 ccgaggctct gaatcgtgcc ccgcgcgtcg aggcacttcc gcccccgccc gacctcagcg
     1681 gcgctggcgc cgtggccagc caggcgacca tcttcgcggc ggtccgtgac gctctggccg
     1741 cacgcgcggc ggcccgcccg ctggccctcc tgctggagga cttgcactgg gccgacccca
     1801 cctcgcttga cctgctccgc gtcgtcgcgc gcggccttgc cgataccccg ctcctgcttc
     1861 tcgccactta tcgcgccgac gagattgatc gtcgccaccc actctacgcc ttgctgccag
     1921 cccttgtccg cgaggcgcgg gccgcacgac tcgacctccg cgcgctagac gaggccgctc
     1981 tgcacgcact ggtcgccgcg cgctacgcgc taccgcctcc cgccgcgacc cggctggtcg
     2041 cctacctgca ctcccgggcc gagggcaacc ccttcttcgc cggtgagctg ctgcgggcac
     2101 tggaggaaga acagcaactc cggcgtgacg gggtggcgtg ggcactgggc gacctgccgg
     2161 cgctggccgt gccgccgctg ctgcgtcagg tgatcgacgc gcggctcgac cggctggggg
     2221 aggcggcccg cgagctgctg gcggtcgcgg ctgtgatcgg ccagaaggtg ccgctggagc
     2281 tctgggccac ggtcgccggg gcgggggagg acgcgctcgt ggacctaact gagcacgcgg
     2341 ctgaggcgca tctcctgctg ccgacgctgg acggggcgcg cttcgcccac gcgctcatcc
     2401 gcgagacgct ctacgagggg acgctgccgc tgcgccggcg ggttcttcac cggcgcgtcg
     2461 ccgaggcgtt gctggccgac cccgaccccg atcccgaggc cgtggcccac catctccagc
     2521 aggccgggga cgcccgcgcc cccgcctggc tggagcaggc gggggcgcgg gcgcagcacg
     2581 ccttcgcctg gctgacggcg accgagcgct acgagtcggc gctggcgctc ctcgagcagc
     2641 gggcggacga cccggcgcgg cgcggctggc tcctcttccg cctggctatg gtgggcggct
     2701 acgccgacga gcggcggagc ctggcctggt tggacgctgc ggtgccgctc ctggcggcgg
     2761 cgcaggaccc cgccctcgcg gcggccgtcc gcttccaccg
//
LOCUS       DASUBI010000071         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_379214, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000071 DASUBI010000000
VERSION     DASUBI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379214"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..2355
                     /locus_tag="VFU72_01285"
     CDS             10..2355
                     /locus_tag="VFU72_01285"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422149.1"
                     /translation="MLRRHRRAAGLSQEALAERAGISARAVQDLERGVHAWPRPDTFR
                     LLADALGLDGPARAELAAAAQPAPDLAAAVLRLDPLPWPPTPLIGRERDVAAACVQLR
                     RPDVRLLTLTGPGGVGKTRLALAIAAELAAAFPDGVAWVDLAPLRDPDLVAPTVARAL
                     GVHESGEQPLADVLTRALAPRRLLLVLDNCEHVLAATAPLVGDVLARGPGVHVLATSR
                     APLHVSGEQERPVAPLSLPDPARLPPRERLHQYAAVRLFVERARAVQPDFAVAHGNAP
                     AVAEICARLDGLPLAIELAAVYVKVVPPPALVRRLDQRLPLLTGGARTLPARQQTMRQ
                     AIGWSHDLLTDAEQTLFRRLAVFTGGCTLAAAEAVVADDEPRAVFGGIAALVDKSLLR
                     QEEGGDGEPRFRMLETVREFALERLAASGGGEALRDRHAAHFRRAASQGAAGLRGPDD
                     LEWSASLEVEHDNFRAALAWLIATDADAAWELASALGEFWYNQSHLSEGRRWLDRVLT
                     LPYSAAPSVTEHDVLHWAGMLAHYQGDYARATPLLTQALAVARALADARRIALTLQGL
                     GIIAEDSGAYVQATQWLDEAVAVHHTVDDRHEEAVTRFHLGIVAYGQGDYRRAEAQCL
                     GARVLAREVGNRPAGRMAALVLAHVAIAVGDLRQAAGWCREVLAIPDEFGGFVARWEG
                     GRLEGPSRLVAGVALLAAAQGAALRAARLFGMAEAARTQIGLVPTLPERAVYARAIEA
                     AKRTVGETSFAQAVDAGRHASAEQVLAEVEAALEAAAQIQG"
     gene            2534..2743
                     /locus_tag="VFU72_01290"
     CDS             2534..2743
                     /locus_tag="VFU72_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422150.1"
                     /translation="MLRSAFAETWQSRDPDIFAQQPQLAAELEQAEAPHVASRADISA
                     GGRGDRGRYRPGSGTRAPHATAGGI"
     gene            complement(2780..3586)
                     /locus_tag="VFU72_01295"
     CDS             complement(2780..3586)
                     /locus_tag="VFU72_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5422151.1"
                     /translation="MVDAAAPVILEAEHLSKRFGAVTALTDVSLRLHRGEVLGLVGDN
                     GAGKSTLIKILCGFHQPDSGSYAFEGKPVHLSSPMEARALGIQTVYQDLALVNDLSVY
                     HNMFLGRERKKRMLGIPLLDNRSMRRLTVEYLSNLGIRIPNVDNTVTMLSGGQRQSIA
                     VARSIYSNPTVLIMDEPLAALGVRESRLVLDLVLELKKRGDISIILIAHNYAQVFEVC
                     DRINFLSGGKIVFDARTADTTVADLIDLVTSGYKRNDPVPAAGAAASEAS"
BASE COUNT          524 a         1272 c         1348 g          548 t
ORIGIN      
        1 tttggaacgg tgctgcggcg ccatcgccgg gcggcggggc tgagccagga agcgctggcc
       61 gaacgcgccg ggatcagcgc ccgcgccgtc caagacctcg aacgcggcgt ccacgcctgg
      121 ccccgccccg acacctttcg cctgttggcc gacgcgctcg ggctcgacgg accggcgcgg
      181 gccgagctgg cggcggccgc ccagccggcg ccggacctcg ccgccgccgt cctccggctt
      241 gaccccctcc cgtggccgcc gacgcccttg atcgggcgcg agcgcgacgt cgccgcggcg
      301 tgtgtccagc tgcgccggcc cgacgtgcgc ctcctgacgc tgaccggtcc cggcggcgtc
      361 ggcaagacgc ggctcgcctt ggcgatcgcg gctgaactgg cggcggcgtt ccccgatggg
      421 gtggcctggg tcgacctggc gccgctgcgc gaccccgacc tggtcgcccc gaccgtggcg
      481 cgggcgctcg gggtgcacga gtcgggcgaa caacccctgg ccgacgtgct gacccgggcg
      541 ctggccccgc ggcgcctcct gcttgtcctc gacaactgcg agcacgtcct cgccgccacc
      601 gccccgctcg tcggcgatgt gctggcccgg ggtccagggg ttcacgtgct ggccaccagc
      661 cgtgcgccgc tccacgtctc cggtgagcag gagcgccccg tcgctcccct gtccctcccg
      721 gatccagccc gcctcccgcc acgagagcgc ttgcaccagt acgcggccgt gcggttgttc
      781 gttgagcggg cacgggcggt gcaaccggac ttcgccgtcg cccatggcaa cgcgcccgcg
      841 gtggccgaaa tctgcgcgcg cctcgatggc ctgccgttgg cgatcgaact ggccgccgtc
      901 tacgtgaaag tcgtgccgcc gccggccctc gtgcgccgcc tcgaccaacg gctgccgctg
      961 ctgaccggcg gcgcccggac gctgccggcg cgccaacaga cgatgcgcca ggccatcggc
     1021 tggagtcacg acctcctcac cgacgcggag caaacgctct tccgccgcct ggcggtcttc
     1081 acgggcggct gcaccctggc ggcggcggaa gcggtggtcg ccgacgacga gccccgggcg
     1141 gtgttcggtg gcatcgccgc cctcgtcgac aagagtctgc tccggcagga ggaagggggc
     1201 gacggggaac cgcggttccg gatgctggag acggtgcgcg agttcgcgtt ggagcggctt
     1261 gcggcgagcg gcggggggga ggcgctccgg gaccgccatg ccgcccattt ccgccgtgcc
     1321 gccagccaag gggcggctgg gctgcggggc ccggacgatc tggagtggag cgcgtcgtta
     1381 gaggtcgagc atgacaactt ccgagcggcg ctcgcgtggc tcatcgccac cgacgccgat
     1441 gccgcctggg aactggcgag cgcgcttggc gagttctggt acaaccagag ccacctcagc
     1501 gaggggcgac gctggctgga tcgcgtgctg acgcttccgt attccgctgc gccgtcggtc
     1561 accgagcacg atgtcttgca ctgggcgggg atgcttgccc actaccaagg cgactatgcg
     1621 cgggcgacgc cgctgctgac ccaggccctc gccgtcgccc gcgctctcgc cgatgcccgg
     1681 cgtatcgcgc tcacgctaca aggcctgggc atcatcgccg aggacagcgg cgcctatgtc
     1741 caagcgacgc agtggctgga cgaggcggtg gcggtccacc acacggtgga cgatcgccac
     1801 gaggaggccg tcacccgctt tcacctcggc atcgtcgcct acgggcaagg ggattaccga
     1861 cgagccgagg cgcaatgcct cggcgcccgg gtgctggcgc gggaggttgg gaaccgcccc
     1921 gcgggccgga tggcggcgct cgtcctggcg cacgtcgcca tcgcggtggg tgacctgaga
     1981 caggcggcgg gctggtgccg cgaggtgctg gccatccccg acgagttcgg cggcttcgtc
     2041 gcgcgttggg agggcggccg tttggaaggg ccatcgcgat tggtggcggg ggtggcgctg
     2101 ctggcggcgg cgcagggcgc ggcgctgcgc gcagctcgct tgttcgggat ggcggaagcc
     2161 gcgcggacac agatcgggct cgtgccgacg ctgccggaga gagcggtcta tgcgcgggcg
     2221 atagaggcgg cgaagcgcac ggtgggcgag acgtccttcg cgcaggccgt tgacgcgggg
     2281 cgccacgcct ccgccgaaca ggtgctggcc gaggtggagg ccgccctcga agccgctgcg
     2341 cagattcagg gctagaatcc gcgacgccga gggcgctccg gaggggacgc ccaccgggcg
     2401 gctctgcgcg ccgcccggcg gcttcgatcc gaacgccaag ctccagcgcg ctccgaacgg
     2461 ctcacggacg agcgggcggc cgccgcgccg atgcgcgtgg gccattggcc tcctcgcggg
     2521 gattgaagga cgcgtgctgc gcagcgcctt tgccgagacc tggcagagcc gcgatccgga
     2581 catcttcgcg cagcagccgc aattggcggc ggagttggag caggccgagg cgccgcacgt
     2641 cgccagcagg gcggacatca gcgccggggg ccggggagat cgtgggaggt atcgtccggg
     2701 aagcggaacc cgtgctccgc atgcgaccgc cggcggcatc tgacacgcag ttgccatcca
     2761 attcggtaaa cggggcggat tatgaggcct cggaagcggc ggcgcctgcc gccggcacgg
     2821 gatcgttgcg cttgtagccc gaggtcacca gatcgatcag gtccgcgacc gtggtgtcgg
     2881 cggtgcgcgc gtcgaagacg atcttgccgc cgctgaggaa attgatgcga tcgcaaacct
     2941 cgaacacctg ggcataattg tgagcgatca ggatgatcga gatatcgccg cgcttcttca
     3001 attccagcac caggtccagc accaggcggc tttcgcgcac gccgagcgcg gcgagcggct
     3061 cgtccatgat cagcacggtg gggttggaat agatggaacg ggccacggcg atgctctggc
     3121 gctgaccgcc ggagagcatc gtgaccgtat tgtcgacatt gggaatgcgg atcccaaggt
     3181 tgctcaaata ctcgacggtg agccggcgca tcgaccgatt gtcgagcaac gggatgccca
     3241 gcatccgttt ctttcgttcc ctgcccagaa acatgttgtg gtagacgctc agatcgttga
     3301 ccagcgccag gtcttggtac acggtttgga tgcccagggc gcgggcctcc atcggcgagc
     3361 tcaggtggac cggcttgcct tcgaaggcat aggagccgct atcgggctgg tggaagccgc
     3421 agagaatctt gatcagcgtg gatttgcccg cgccgttgtc tcccaccagc cccaacacct
     3481 cgccccgatg caggcggagg ctgacgtccg tcagggccgt gaccgcgccg aagcgcttgc
     3541 tcagatgctc ggcctcgagg atgaccggag cggcagcatc gaccatcagc gcttcctccg
     3601 cacccgcacc gcgtccactt gcacattgag gatcatggcc gcaaggatcg cggcgccgag
     3661 gtaaatgttg gactccgtgg cttgggcgcc ca
//
LOCUS       DASUBI010000072         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1305384, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000072 DASUBI010000000
VERSION     DASUBI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305384"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFU72_01300"
     CDS             <1..740
                     /locus_tag="VFU72_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422152.1"
                     /translation="VNNPFMWTADDKFFEAALATALGIASPKTLVLPNKEYVPGIVPS
                     ESLRNLIYPLDWQAIVDYVGLPCVLKDAHGGGWKDVFVCKTMDDLLRHYNDSGLRTMI
                     LQEFIEWDHYVRCICLGQQEVLPIKYDPNERKYHIDHQHLTSDMGQRIMEDSLKLVSA
                     LGYDMNSVEWAIRDGVPYAIDFMNPAPDMDIYSLTPHYFEWAVSHMADMAIRLAKSPP
                     KQRQRLDWSDLFTYSREAGQGARATGS"
     gene            758..2110
                     /locus_tag="VFU72_01305"
     CDS             758..2110
                     /locus_tag="VFU72_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422153.1"
                     /translation="MELREAIERYHDLLTPELAAASQEQLDEQQQRRGLFFGTRPLCT
                     VLRPRFLTHDQYRFLEERVALLLGAFDVAYQAAVSEPELRAQFMLDETEEELVRERFG
                     FRDPSPTSRLDTFFIPGTGQLHVVEYNAEVPAAIAYNHAVSEIMLALPVMRAFLKRYE
                     VRPLSSRHMLLGVLLDAFEQWAGRREKPSIAILDWEDVPTYSEFILFRDYFQQQGYAC
                     VIADPRQLEYRGGVLYAADTPVNLIYKRVLISELIQRGRLDQPVIRAVRDGAACMVNP
                     FACKILHKKASLAVLSDERNHHLFSPDQVQAIAAHIPWTRRVVDERTSYQGQEVELVP
                     FILANRDRLVLKANDEYGGKGVVLGWETDAAGWEWAVQTALSEPSVVQERVPVDSEPF
                     PSLIEGELRVIDRMLDTDPFVYHGAFMDGALTRISTAALLNVTAGGGSTVPTMLVEER
                     "
     gene            2131..3276
                     /locus_tag="VFU72_01310"
     CDS             2131..3276
                     /locus_tag="VFU72_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEU5422154.1"
                     /translation="MLRQPSLNIGIEEEYQIIDPETRELRSYIREILQDDHVVLEQVK
                     PELHQSIVEVGTEVWETAADAREDLIRLRRLVMELVARSGLRIAAAGTHPFSSWLEQE
                     ITPLERYAGVKEDMQDLAQQLLIFGTHVHIGIEDREFLVHAMNVSRYFLPHVLALSAS
                     SPFWMGRDTGLKSYRSVVFRAFPRTGIPPEFESWPQLHSIVDTLVETSSIPDASKIWW
                     DVRPNPKYPTLEFRICDVCTRVDEAVAVAAIFQAIVAKIWKLRRDNLTFRVYPSTLIE
                     ENKWRVVRRGLDGKLIDFGKREQVAARALERELIEWFVDDVLDDLGSRKEVEYAFQIL
                     DEGTSADRQLAVFRRTGSLRAVVDELIRETAEGVLPVPAGEQTGGGV"
     gene            3276..4481
                     /locus_tag="VFU72_01315"
     CDS             3276..4481
                     /locus_tag="VFU72_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422155.1"
                     /translation="MANGDGLVVLCMASHEKGQELIRECKRQGSHVILLTTTEFEHGD
                     WPRDSIDEFYTMPNLYNLENVTNGVSYLMRERPIARIIPLDDFDVETAAWLRQHLQLP
                     GIGTTIANHFRDKLAMRTSARLGGVSEPEFVGVLNYDQIREFMARVPPPWFMKPRGEA
                     SAVGIRRLETPDQFWSAVEPLGDQQSHYLMEQYVAGQVFHVDSIVWDGEVIFAEAHRY
                     GHPPFDVMHGGGIFTSRPIKRGTATDQALKEMNAAAIAALGLPRGVTHLEAIQGQDDG
                     RFYFLECAARVGGANIADGIELATGINLWSEWAKIELSQGSTPYTLPARREEYSGIII
                     TLARQQFPDLSGYTEPEIAWRMNKEHHAGLIVVSPDPSRINQLLDHYLDRFYQDFHAS
                     MRPPDSLRE"
BASE COUNT          816 a         1633 c         1562 g          754 t
ORIGIN      
        1 tcgtcaacaa tcccttcatg tggaccgccg acgacaaatt cttcgaggcg gcgctggcca
       61 ccgcgctcgg catcgccagc cccaagacgc tggtgctgcc gaacaaggag tacgtgcccg
      121 gcatcgtgcc cagtgagagc ctgcgcaacc tgatctaccc gctcgactgg caggcgatcg
      181 tcgactacgt cggtctgccc tgcgtgctca aggacgccca cggcggcggc tggaaggatg
      241 tcttcgtctg caagacgatg gacgacctcc tccgccacta caacgactcc ggcctgcgca
      301 ccatgatcct gcaggagttc atcgagtggg accactacgt gcgctgcatc tgcctcggcc
      361 agcaggaggt gctgccgatc aagtacgacc ccaacgagcg caagtaccac atcgaccacc
      421 agcacctgac ctcggacatg ggccagcgca tcatggagga ctcgctcaag ctcgtcagcg
      481 ctctcggcta cgacatgaac tcggtcgagt gggctatccg cgacggcgtg ccctacgcca
      541 tcgacttcat gaacccggcg ccggacatgg acatctactc gctgacgccc cactacttcg
      601 agtgggcggt cagccacatg gccgacatgg cgatccgcct ggccaagagc ccgcccaagc
      661 agcgccagcg gctcgactgg tccgatctct tcacctacag ccgcgaggcg ggacagggcg
      721 cgcgggccac cgggtcgtag ccgaaagggg gcgggagatg gagctgcggg aggcgatcga
      781 gcgctatcac gacctgctga cgccggagct ggccgccgcc tcgcaggagc agctcgacga
      841 gcagcagcag cggcgcggtc tcttcttcgg cacgcgcccg ctctgcaccg tgctgcgccc
      901 tcgcttcctc acccacgacc agtaccgctt tctcgaggag cgcgtcgcgc tgctgctggg
      961 cgccttcgat gtggcctacc aggccgccgt cagcgagccc gagttacgcg cccagttcat
     1021 gctcgatgag actgaagagg aactggttcg cgagcgcttc ggctttcgcg atccaagccc
     1081 gacctcgcgc ctggacacct tcttcatccc cggcaccggg cagctccacg tggtcgagta
     1141 caacgccgag gtgccagccg ccatcgccta caaccacgcc gtctccgaga tcatgctggc
     1201 gctgccggtg atgcgcgcgt tcctcaagcg ctacgaggtg cgcccgctct cctcgcgcca
     1261 catgctgctg ggcgtgctgc tggatgcctt cgagcagtgg gccggccggc gcgagaagcc
     1321 gagcatcgcc atcctcgact gggaggacgt gccgacatac agcgaattca tcctcttccg
     1381 cgactacttc cagcagcagg ggtacgcctg cgtcatcgcc gatccacggc agctggaata
     1441 ccgcggcggc gtcctctatg ccgccgatac gccggtcaac ctgatctaca agcgcgtgct
     1501 gatcagcgag ctgatccagc gcgggcggct cgaccagccg gtcatccgcg cggtgcgcga
     1561 cggcgccgcc tgcatggtca accccttcgc ctgcaagatc ctgcacaaga aggccagcct
     1621 ggccgtgctc agcgacgagc gcaaccacca cctcttctcg cccgatcagg tgcaggcgat
     1681 cgccgcgcac atcccctgga cccggcgcgt ggttgatgag cgcacgtcgt accagggaca
     1741 ggaggtcgag ctggtcccct tcatcctggc caaccgcgac cgtctggtgc tgaaggccaa
     1801 cgacgagtac ggcggcaagg gggtcgtgct tggctgggag accgacgccg ccggctggga
     1861 gtgggcggtg cagacggcgc tttccgagcc cagcgtggtg caggagcgcg tgccggtcga
     1921 tagcgagccc ttccccagcc tgatcgaagg cgagctgcga gtcatcgacc gcatgctcga
     1981 caccgacccc ttcgtctacc acggcgcgtt catggacggc gcgttgaccc gcatctccac
     2041 cgccgcgctg ctcaacgtga cggcgggcgg cggctcgacg gtccccacca tgctggtcga
     2101 ggagcgctag caccggcaga ggaggtcgcc atgctcaggc aaccatcgct caacatcggc
     2161 atcgaagagg agtaccagat catcgacccg gagacgcgcg agctgcgctc ctacatccgg
     2221 gagatcctgc aggacgacca cgtcgtgctg gagcaggtca agccggagct gcaccagtcg
     2281 atcgtcgagg tcggcaccga ggtctgggag acggcggccg acgcgcgcga ggatctgatc
     2341 cgcctgcgcc gcctggtgat ggagctggtc gcgcgtagcg ggctgcgtat cgcagccgct
     2401 ggcacacatc ccttctcctc ctggctggag caggagatca ccccgctgga gcgctatgcc
     2461 ggtgtgaaag aagatatgca ggatctggcg cagcagctcc tgatcttcgg cacgcacgtg
     2521 cacatcggta tcgaagaccg cgagttcctg gtgcacgcga tgaacgtctc gcgctacttc
     2581 ctgccgcacg tgctggcgct ctcggccagc tcacccttct ggatgggccg cgacaccggc
     2641 ctcaagtcgt accgcagcgt cgtcttccgt gccttcccgc gcaccggcat cccgccggag
     2701 ttcgagtcgt ggccgcagtt gcactcgatc gtcgatacgc tggtcgagac gtcctcgatc
     2761 ccggacgcca gcaagatctg gtgggacgtc cggcccaacc ccaagtaccc cacgctggag
     2821 ttccgcatct gcgacgtctg cacgcgggtc gatgaggcgg tggcggtggc ggctatcttc
     2881 caggcgatcg tggccaagat ctggaagctg cgccgcgaca acctgacctt ccgcgtctac
     2941 ccctccacgc tgatcgagga gaacaagtgg cgtgtggtgc gccgcgggct cgacggcaag
     3001 ctgatcgact tcggcaagcg cgagcaggtg gcggcgcggg cgctggagcg cgagctgatc
     3061 gagtggttcg tcgacgacgt gctcgacgac ctggggagcc gcaaggaggt cgagtatgcc
     3121 ttccagatct tggacgaggg taccagcgcc gaccgccagt tggccgtctt ccgccgcacc
     3181 ggcagcctgc gggcggtggt cgacgagttg atccgcgaga cggccgaggg cgtgctgccg
     3241 gtgccggcgg gggaacaaac gggcggaggt gtctgatggc gaacggcgac gggttggtcg
     3301 tgctctgcat ggcgagccac gagaagggcc aggagctgat ccgcgagtgc aagcggcagg
     3361 gcagccacgt gatcctgctg accaccaccg agttcgagca cggcgactgg ccgcgcgaca
     3421 gcatcgacga gttctacacc atgccgaacc tctacaacct ggagaacgtc accaacggcg
     3481 tcagctatct catgcgcgag cgcccgatcg cacgcatcat cccgctggac gacttcgacg
     3541 tggagacggc cgcctggctg cgccagcatc ttcagctgcc gggcatcggc accaccatcg
     3601 ccaaccactt ccgcgacaag ctggccatgc gcacctcggc gcggctgggc ggagtgagcg
     3661 agccggagtt cgtcggcgtg ctcaactacg accagattcg cgaattcatg gcgcgcgttc
     3721 cgccgccctg gttcatgaag ccgcgcggcg aggcctcggc ggtcgggatt cggcggctgg
     3781 agacgcccga ccagttctgg agcgccgtcg agccgctggg cgaccagcag tcgcactacc
     3841 tcatggagca atacgtcgcc gggcaggtct tccacgtcga ctcgatcgtc tgggatggtg
     3901 aggtgatctt cgccgaggca caccgctacg gccacccgcc cttcgacgtg atgcacggcg
     3961 gcggcatctt caccagccgc ccgatcaagc gcggcaccgc aaccgatcag gcgctgaagg
     4021 agatgaacgc ggcggcgatc gcggcgctcg gcctgccgcg cggggtgacg cacctggagg
     4081 cgatccaggg gcaggacgac gggcgtttct acttcctgga gtgcgcggcg cgcgtcggcg
     4141 gcgccaacat cgccgacggc atcgagctgg cgaccgggat caatctctgg agcgagtggg
     4201 ccaagatcga gctgagccag gggagcacac cctacacgct gcccgcgcgg cgcgaggagt
     4261 actccggcat catcatcacc ctggcgcggc agcagttccc cgatctctcg ggctacaccg
     4321 agccggagat cgcctggcgc atgaacaagg agcaccacgc ggggctgatc gtggtctcgc
     4381 ccgacccctc gcggatcaat caactgctcg accactacct cgatcgcttc taccaggact
     4441 tccacgcgtc catgcgcccg ccggactcgc tgcgggagtg aggggccctc atccccaacc
     4501 ccttctccca atactgggag aaggggagac ggtacggttg taggggcagg cctcggtgtc
     4561 tgccctcgtc gccctacgca ccggcaccgg ggaatgggcg aaacgcgggt ggcgcttatg
     4621 gggccaaatg gtttgccgcg agggcaggca cggaggtctg cccctacctc tgggccgtct
     4681 ttgcctgcgc cttgacggcg ggcgcgacct tcgacgcgat cagctccatg gtgcgccagg
     4741 cgtcggggcc ctgggaggcc gacat
//
LOCUS       DASUBI010000073         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_120604, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000073 DASUBI010000000
VERSION     DASUBI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120604"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFU72_01320"
     CDS             <1..827
                     /locus_tag="VFU72_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422156.1"
                     /translation="VDGVDADVLRRLAAETHTVFSGVEEPFAVPLRRQNRPRIGLYRG
                     WRPNGIDEGWTRFIFERAGFAFEPVRDRDLRQGNLRARFDVILLPQQSPRDILEGNNI
                     KDYPSEYAGGIGEQGAVNLRRFTEAGGTLIALDAACEVAIRALYLPIANALENLPSDT
                     FYSPGSVVQLLLDNQHPVAWGYGREVAALFVSSPAFSVLNGAEVVARYPFSDPLLSGW
                     MLGAEYLEGKAALVDVPVGAGHALLFGFRPQFRAQTRGTYRLLFNALYYATLEPPE"
     gene            824..1558
                     /locus_tag="VFU72_01325"
     CDS             824..1558
                     /locus_tag="VFU72_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422157.1"
                     /translation="MTSYDLYLESGPRRRKTMVHVLDLLGCVALGPTTEEALAATPDA
                     IRAYRRFLRGHGEPLDPDEPFQTQVAEHITEGDWLGNGSPYLVFRPDLAPLTEEEIDT
                     YLRWFRWLRDDLAAWAARRTPAQLDATPVAGGRSARAILLHVLGATGGYLSAALGSAK
                     GFSALHGAAERGEIALHDALRHAAVMAEERVRATTAEERGAVRELPTGPRTLRKALRR
                     LLEHDWEHLSELARRPDGPALRHEDL"
     gene            1631..2914
                     /locus_tag="VFU72_01330"
     CDS             1631..2914
                     /locus_tag="VFU72_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5422158.1"
                     /translation="MDRTIPRGGAEAAPQPPPYRPVESGESVVHLATVLRVLMYSGLA
                     IILLGTALALVRHGNLPTPVTNVTALPGQLAGLHSDAILSLGILIFLCSPAAALAYLT
                     VSYLLARNRLYTVIAAVVLAIVLGSIVVARYSGAGAGVESGPELTLAREAAIFLLSLS
                     GGILGSMVGIGGGIFMVPVLTAFVGIPIKTAIAASAVSVIVNSIGGSSVYLQHKLTNV
                     RLALFMELTTAVGAIAGGLLVVYIAGNPLRVIFALALVGMGLAMLRQQKQQAPATSGD
                     DPLRLAQVFHDPVDQADVVYIPQRLPLGAVAGFLAGVISGLLGIGGGAIKIPIMNALM
                     RVPVKAAAATSILMVGITVSASAFIYYIHDLIDLSVTIPAVLGILIGSQTGAAVSRHL
                     KSVTLVRVLVLIVVYLAINLLLQALGIRLPGTSGS"
     gene            complement(3034..>3338)
                     /locus_tag="VFU72_01335"
     CDS             complement(3034..>3338)
                     /locus_tag="VFU72_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422159.1"
                     /translation="DAATAGTGCEYVAATGHSLCGAFRDEWHAHGLDLGDRGLSQRES
                     LALFGYPISEPFTDPGSGLMVQYFERARFESRPDNPAGWQVELGRLGADHLSERGW"
BASE COUNT          448 a         1208 c         1153 g          529 t
ORIGIN      
        1 cggtcgacgg agtcgacgcg gacgtgctgc ggcgcctggc cgccgagacg cataccgtct
       61 tcagcggcgt cgaggagccc ttcgcggtgc cgctgcggcg ccagaaccgg ccgcgcatcg
      121 gcctctatcg cggctggcgc ccgaacggca tcgacgaggg ctggacgcgc ttcatcttcg
      181 agcgcgcggg cttcgccttc gagccggtgc gcgaccgcga cctgcgccag gggaacctgc
      241 gcgcgcgctt cgacgtgatc ctgctgcccc agcagtcgcc gcgcgacatc ctggagggga
      301 acaacatcaa ggactacccc agcgagtacg ccgggggcat cggtgagcag ggcgcggtta
      361 acctgcgccg ttttaccgag gccggcggca cactgatcgc cctggacgcc gcctgcgagg
      421 tcgccatccg tgccctctac ctgccgatcg ccaacgcgct ggagaacctg cccagcgaca
      481 ccttctacag ccccggctcg gtcgtccagc tcctgttgga caaccagcac ccggtcgcct
      541 ggggctacgg gcgcgaggtc gcggcgctct tcgtcagcag cccggccttc agcgtgctca
      601 acggcgccga ggtagtggcg cgctacccct tcagcgaccc gctcctctcc ggctggatgc
      661 tcggcgcgga atacctggag ggcaaggcgg cgctggtcga cgtgccggtc ggcgccgggc
      721 acgcgctgct cttcggcttc cggccgcagt tccgcgccca gacgcgcggc acctatcgcc
      781 tgctgttcaa cgcgctctac tacgccacct tggagccgcc tgagtgacga gctacgatct
      841 ctacctggag tcgggtccgc gccggcgcaa gacgatggtc cacgtgctcg acctgctcgg
      901 ctgcgtcgcg ctcggcccga cgaccgagga ggcgctggcg gcgacgcccg acgcgatccg
      961 tgcctatcgt cgcttcctgc gggggcacgg cgagccgctc gaccccgacg agccgttcca
     1021 gacccaggtc gccgagcaca tcaccgaggg tgattggctc ggcaacggct cgccctacct
     1081 cgtcttccgg cccgatctgg cgccgctcac ggaggaggag atcgacacct acctgcgctg
     1141 gttccgctgg ctgcgcgacg atctggcggc ctgggccgcg cgccggacgc ccgcgcagct
     1201 cgacgcgacg ccggtggccg gcggccgctc cgcgcgcgcc attctgctcc acgtgctcgg
     1261 tgccaccggc ggctatctct cggcggcgtt aggcagcgcg aagggcttct cggcgctgca
     1321 tggggcggcg gagcgtgggg agattgcgct gcacgacgca ctccggcacg cggcggtgat
     1381 ggccgaggag cgcgtacgcg ccaccacggc ggaggagcgc ggcgcggtgc gcgagctgcc
     1441 gacgggtccg cgaaccttgc gtaaggcgct gcggcgcctg ctcgagcacg attgggagca
     1501 tctgtccgag ctggcccgtc gaccggacgg gccggcgttg agacacgaag acctctgatc
     1561 tgccgtcgac gcggctgacg atctgcccgc tgcaggccgg cctgcgctga caagggaacg
     1621 agcgagcgtg atggatcgaa caattccgcg tggcggagca gaagccgccc cccaaccgcc
     1681 gccgtaccgc ccggtcgaga gcggcgagag cgtcgttcac ctggcgacgg tgctccgtgt
     1741 gctgatgtac tccgggctag cgatcatcct gctcggcacg gcgctggcgc tggtgcgcca
     1801 cggcaacctg ccgacgccgg tgacgaatgt caccgcgcta cccggccagc tcgccggact
     1861 gcactccgac gcgatcctct cactcggcat cctgatcttc ctctgctcgc ctgcggccgc
     1921 gctcgcctac ctgaccgtct cctatctgct ggcgcgcaac cggctctaca ccgtgatcgc
     1981 ggcggtcgtg ctggcgatcg tgctcggcag catcgtggtg gcgcgctact ccggcgcggg
     2041 ggccggtgtc gagtccggcc cggagctgac cctggcgcgc gaggcggcga tcttcctgct
     2101 gtcgctgagc ggcggcatcc tcggctcgat ggtcggcatc ggcggcggca tcttcatggt
     2161 cccggtgctg acggccttcg tcggcatccc gatcaagacc gccatcgcgg ccagcgcggt
     2221 cagcgtgatc gtcaactcga tcggcggcag ctcggtctac ctgcagcaca agctgaccaa
     2281 cgtgcgcctg gcgctcttca tggagctgac gaccgccgtc ggcgccatcg ccggcggcct
     2341 gctggtcgtc tacatcgcgg gcaacccgct gcgggtcatc ttcgcgctgg cgctggtggg
     2401 gatggggctg gccatgctgc ggcagcagaa gcagcaggcg ccggccacca gcggcgacga
     2461 cccgttgcgg ctggcccagg tcttccacga cccggtggac caggccgacg tcgtctacat
     2521 cccgcagcgg ctgccgctcg gcgccgtggc cggcttcctg gccggggtca tctccgggct
     2581 gctcggcatc ggcggcggtg cgatcaagat cccgatcatg aatgcgctga tgcgggtgcc
     2641 ggtcaaggcc gccgccgcca ccagcatcct gatggtcggc atcaccgtct cggccagcgc
     2701 cttcatctac tatatccacg acctgatcga cctctcggtc accatcccgg ccgtgctggg
     2761 tatcctgatc ggctcgcaga ccggcgccgc cgtctcgcgc cacctcaaga gcgtcacgct
     2821 ggtgcgcgtg ctggtgctga tcgtggtcta tctggcgatc aacctgctac tgcaggcgct
     2881 gggcatccgc ctcccgggca cgtcggggtc gtaggcggga cggccctcat ccccccgccc
     2941 cttctcccaa tactgggcga aggggagcat agtacaccca tccttcgtgc tccctctccc
     3001 agcattggga gagggctggg gtgagggctc gtcctaccag ccacgctcgc tcaggtggtc
     3061 ggcgccgagg cggccgagct cgacctgcca gccggccggg ttgtccgggc gcgactcgaa
     3121 gcgggcgcgc tcgaagtact gcaccatcag gccactgccg gggtcggtga agggctcgct
     3181 gatcgggtag ccgaagagcg ccagcgactc ccgctgcgag aggccgcggt cgcccaggtc
     3241 tagcccgtgc gcgtgccact cgtcgcggaa ggcgccgcag aggctgtgac cggtggcggc
     3301 gacgtactcg cagcccgtgc cagcggtcgc ggcgtcta
//
LOCUS       DASUBI010000074         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_338332, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000074 DASUBI010000000
VERSION     DASUBI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338332"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="murD"
                     /locus_tag="VFU72_01340"
     CDS             complement(<1..914)
                     /gene="murD"
                     /locus_tag="VFU72_01340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5422160.1"
                     /translation="MASWLAGRRALVMGLGTHDGGLGVARYLARQGAEVTVTDLRGPE
                     ALGPALEALRGLPLRFTLGEHRAEDFERAELVVRNPAVPLDSPWLALARAHGASIEME
                     MSLFFRACPAPIVGITGTKGKTTTATLCAEILRRWRRDTVLAGNMGKSALETLQQITP
                     ETPVVLELSSWQLEGLVEPGLSPHIAVFTNFSEDHLNRYPSMAAYLDAKLQIARCQGP
                     DDFFVVNRDDPAVWGARGVTAGTVLPFGLDDGESDGAFLRGERLLWRRQGDEAEICRV
                     DELALPGAHNARNALAAAAAAMLRGAAP"
     gene            complement(927..1964)
                     /gene="mraY"
                     /locus_tag="VFU72_01345"
     CDS             complement(927..1964)
                     /gene="mraY"
                     /locus_tag="VFU72_01345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5422161.1"
                     /translation="MILADLAIIVGHSLARGGILPREPGHNLAASLGLSAVAFAVTVA
                     VGRPYITYLREHGIGKQIRIEEPEGHSLKMGTPTMGGILFTSVVILLTIVFNLVGRLS
                     MLLPLGVLIGTAVLGGIDDRLNLVGGKSTGMTARFKMAWLLVFATVTALILYFPLHLR
                     SVYIPLLGKYDIGLIYIPIAIFVISGSANAVNLTDGLDTLAGGTAAVAFVAYGIIAFL
                     QGQIQVVTFCFTMAGALMGFLWYNAHPAQVFMGDTGSLAIGASLATAAFMTGQWLLLP
                     VVGIVFMAETLSVMIQVAYFKRTGGKRVFRMTPLHLHFELLGWSETQITLRFWLVGML
                     AGLVGVALALL"
     gene            complement(1961..>2235)
                     /locus_tag="VFU72_01350"
     CDS             complement(1961..>2235)
                     /locus_tag="VFU72_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5422162.1"
                     /translation="EAEGHRLVGERAAVVLDELLTYGPRARLLARAAVAGGLPAAQVR
                     SFDDKPALVDALREMLRPGDVVLVKGSRGMTMETVVEAIRARKAAS"
BASE COUNT          377 a          800 c          763 g          295 t
ORIGIN      
        1 tcaggcgcgg ccccgcgcag catcgccgcg gccgccgccg ccagcgcgtt gcgcgcgttg
       61 tgcgcccccg gcagcgccag ctcatctacg cggcagatct ccgcctcatc accctgccgg
      121 cgccagagca ggcgctcgcc gcgcaggaac gcgccgtccg actctccgtc gtccaggcca
      181 aacggcagca ccgtcccggc cgtcaccccc cgcgcgcccc agaccgccgg atcgtcgcgg
      241 ttgaccacga agaagtcgtc cggcccctgg cagcgggcga tctgcagctt ggcatcgagg
      301 tacgcggcca tcgagggata gcggttgagg tgatcctcgc tgaaattggt gaagacggcg
      361 atgtgggggc tgagccccgg ctcgaccagc ccttcgagct gccagctcga cagctccagc
      421 accaccggcg tctcgggtgt gatctgctgc agcgtctcca gcgcgctctt gcccatattg
      481 ccggccagca cggtgtcccg ccgccagcgg cgcaggatct cggcgcagag cgttgccgtg
      541 gtcgtcttcc ccttggtgcc ggtgatgccg acgatcggcg cggggcaggc gcggaagaaa
      601 aggctcatct ccatctcgat gctggcgccg tgggcgcgcg ccagcgccag ccagggggag
      661 tcgagcggca ccgccgggtt gcgtaccacc agctcggcac gctcgaagtc ctcggcgcgg
      721 tgctcgccca gggtgaagcg cagcggcagg ccgcgcagcg cctccagcgc cgggccgagc
      781 gcctcggggc cgcgcaggtc ggtgacggtc acctcggcac cctggcgcgc caggtagcgc
      841 gccacaccca ggccgccgtc gtgcgtcccc aggcccatca ccagcgcgcg tcgccccgcc
      901 agccagcttg ccaccgatca gtcctcctag agcagcgcca gcgccacgcc aacgagcccg
      961 gccagcatcc ccaccagcca gaagcgcagc gtgatctggg tctccgacca gcccagcagc
     1021 tcgaagtgca ggtgcagcgg cgtcatgcgg aagacgcgct tgccgccggt gcgcttgaag
     1081 taggcgacct ggatcatgac cgagagcgtc tcagccatga agacgatgcc gacgaccggc
     1141 aagaggagcc actggccggt catgaaggcg gcggtggcca gcgaagcgcc gatcgccagc
     1201 gagccggtgt cacccatgaa gacctgcgcc gggtgcgcgt tgtaccagag gaagcccatc
     1261 aacgcgccgg ccatggtgaa gcagaaggtg acgacctgaa tctgcccctg caagaaggcg
     1321 atgatgccgt aggccacgaa ggccaccgcc gccgtgccgc ccgccagcgt gtcgaggcca
     1381 tcggtcaggt tgacggcgtt ggccgagccg gagatgacga agatggcgat gggaatatag
     1441 atcagtccga tatcgtattt gccgagcagc gggatataga cgctgcgcag gtgcagcggg
     1501 aagtagagga tcagcgcggt gacggtggcg aagacaagca gccaggccat cttgaagcgg
     1561 gcggtcatgc cggtcgactt gccgccgacc agattcaagc ggtcgtcgat gccgccgagg
     1621 accgccgtgc cgatcagcac gccgagcggc aggagcatcg agagccgccc gaccagattg
     1681 aagacaatcg ttagcaggat cacgacgctg gtgaagagga taccgcccat ggtgggtgtg
     1741 cccatcttga gcgagtgccc ctccggctcc tcgatacgga tctgtttgcc gatgccgtgc
     1801 tcgcgcaggt aggtgatgta gggtcgcccg accgcgaccg tgaccgcgaa ggccaccgcc
     1861 gagagaccga gcgaggccgc caggttgtgg ccgggctcgc gcggcaggat gccgccgcgc
     1921 gccagcgagt gaccgacgat gatggccaga tccgccagga tcacgacgcc gcctttcgcg
     1981 cccggatggc ctcgaccacc gtctccatcg tcatgccgcg cgagcccttg accagcacca
     2041 cgtcgccggg ccgcagcatc tcgcgcaagg catccaccag cgccggcttg tcgtcgaacg
     2101 agcgcacctg cgcagccggc aggccgcccg ccaccgccgc ccgggccagc aggcgggcgc
     2161 gcgggccgta ggtcagcagc tcgtcgagca ccaccgccgc gcgctcgccc accagccggt
     2221 gcccctcggc ctcgt
//
LOCUS       DASUBI010000075         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_175571, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000075 DASUBI010000000
VERSION     DASUBI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175571"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFU72_01355"
     CDS             complement(<1..658)
                     /locus_tag="VFU72_01355"
                     /inference="COORDINATES: protein motif:HMM:NF036526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5422163.1"
                     /translation="MAIEAAGGRPPEDLTARTRIRDAALRLFTECGISGATIRGIAAV
                     AGVSPGLVRDHFGSKEALRDACDAYALERLTRLQAEIFDEGQLDDQGFLFSIQPTVLL
                     LQNYLVRSMMDGSDAAAAMFETAVQLGERWLEREQIESSDPRAFAAALVSMKLGVFLL
                     HEQLSRVLGVDIRTPEGHARVLKGMLDAFTHPLLTAEESARTHAAVDQLLAISTHTRR
                     A"
     gene            complement(956..1615)
                     /locus_tag="VFU72_01360"
     CDS             complement(956..1615)
                     /locus_tag="VFU72_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422164.1"
                     /translation="MSGADRPPDGPIKRQVQFAGRLWDIRQGDSGGNHFEATNVVVDV
                     HGRLFLGIARHAGRWTSAEITLTEPAGYGAYAIVVDSPLALARNVVLSFALRRDDEHE
                     LAIQLSPWWGRPSNAQFLVQEAESLPEGWLETFRLPEDRAPTEHELRWSPESVEFASY
                     DAQPRLLRGRRLVHEARLALAVEPEGYRLAVGLWLFRGAPPGDGRDQRLWVREVAIKE
                     L"
     gene            complement(1618..2079)
                     /locus_tag="VFU72_01365"
     CDS             complement(1618..2079)
                     /locus_tag="VFU72_01365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5422165.1"
                     /translation="MSEPHVEAVAWFREKFRPFVEGQRLEVAERAPGEFVVRLPQAAP
                     WQRGGGGTDALNGGVIAYMFDGSMGGAIASAVLARVDWETFDLTQFGQVTVSLTINYL
                     RAALGDRFEAHGSVTKLGRGMAFAEGRFYDERGELCATATGVWRLFLPQDG"
     gene            complement(2076..2855)
                     /locus_tag="VFU72_01370"
     CDS             complement(2076..2855)
                     /locus_tag="VFU72_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5422166.1"
                     /translation="MAGQSTNAYLVGERELLIVDPGSEPGVALIQEALAARAGVPVRA
                     IVLTHAHPDHAQAAVPLKRLLGVPLLLHPGNQPILGRSLTWDEVDQELADGMVLEIDG
                     GRLEAVLTPGHAPGHIALFEPSSGTLLAGDLVSGNGTVGIFPPHGSMVEYLASLARAR
                     ALGVGRILPGHGPAIPEGAARLDEYIEHRLNREREVYERISRQPATIEAIVIDLYPDV
                     LPQFRRAAGATVLAHLQKLQGERSVRPEHEAEPFTTIWRAT"
     gene            3220..3564
                     /locus_tag="VFU72_01375"
     CDS             3220..3564
                     /locus_tag="VFU72_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5422167.1"
                     /translation="MKYLLMLFDGDGTSARQQTDDLPTIIAAHERFTDELRAGGQYVA
                     AGGLQPRETATTVRVRNGETLISDGPFAEAKEQVGGFYMVEAADLDEALALAARVPYT
                     DTYAAIEIRPLF"
     gene            3564..>4138
                     /locus_tag="VFU72_01380"
     CDS             3564..>4138
                     /locus_tag="VFU72_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5422168.1"
                     /translation="MTGEHSTTSRPAGDAGREDSPANPAGVAIAAVFRQEHGRIIAGL
                     IRRLGDFDRAEEALQDAFAAALERWPDEGTPRNPAAWLTTTARRKAIDRARREQLQAA
                     KYEAFGREPGGEARDEVEYLAEEDESGSIPDDRLRLIFTCCHPALGLEAQVALALRTL
                     GGLTTGEIARAFLLPEATVAQRLVRAKRKIR"
BASE COUNT          627 a         1451 c         1440 g          620 t
ORIGIN      
        1 cggcgcgccg cgtgtgcgtg ctgatcgcga gtagctggtc taccgccgcg tgtgtccggg
       61 cggattcctc cgcggtcagc aacgggtggg taaaggcgtc gagcatccct ttgagcacgc
      121 gcgcgtggcc ctccggggta cggatgtcga ccccgagcac gcgggacaat tgctcgtgca
      181 gcaggaagac gccgagcttc atggatacca gggccgcggc gaaggcgcgc gggtcggacg
      241 actcgatctg ctcgcgctcc agccagcgct caccgagctg gaccgcggtc tcgaacatcg
      301 ccgcggccgc gtccgagcca tccatcattg agcgcaccag gtagttctgc agcaggagca
      361 cggtgggctg aattgagaac aggaatccct ggtcgtccaa ctgcccctcg tcaaagatct
      421 ccgcctgcag ccgcgtgagc cgctccagcg cgtaggcgtc gcaggcgtcg cgcagcgcct
      481 ccttcgagcc gaagtgatcc cgcaccagtc ccggcgagac gccggccacg gccgcgattc
      541 cccggatagt ggcgccgctg atgccgcact cggtgaagag tcgcagggcc gcgtcacgga
      601 tgcgcgtgcg cgccgtcaga tcctcgggtg ggcgccctcc ggctgcttcg atcgccatcg
      661 ccgcctcctg ttcaggcgtg ccggaaccta ttcgcgacgg caatgtacta tacgtgtgta
      721 tagtcgtcaa gaggcgtgcg cgtagcggtc gagaaggatg gatggcatcc aggatggatc
      781 cttcgactgc gctcagagcc tgccctgagc ctgccgaagg gatgacagga agagtgcggg
      841 gcggacggcg acggctcgtg tcgcgcggta ggccactgcc tctgccctgt catcctgagc
      901 ttgtcgaagg atccgtcctc gatggtgctc agcgtagtcg gagggtgcgg cccgcctaca
      961 gctccttgat cgccacctcg cggacccaga ggcgctggtc gcggccgtcg ccggggggag
     1021 cgccgcgaaa gagccagagg ccgacggcca ggcggtagcc ctccggctcc acagcgaggg
     1081 cgagccgcgc ctcgtgcacc aggcggcgcc cgcgcaagag ccgcggctgg gcgtcgtagc
     1141 tggcgaactc cacgctctcg gggctccagc gcagctcgtg ctccgtggga gcacggtcct
     1201 ccggcagacg gaaggtctcc agccagccct ccggcaggct ctcggcctcc tggaccagga
     1261 attgggcgtt gctggggcgg ccccaccagg gggagagctg gatagccagc tcgtgctcgt
     1321 cgtcgcggcg cagcgcgaag ctgagcacga cgttgcgcgc cagcgcgagc gggctatcga
     1381 ccacgatggc gtacgcgccg tagccggccg gctcggtcag ggtgatctcg gccgaggtcc
     1441 agcggccggc gtggcgcgcg atgcccagga agagccgccc gtggacatcg acgactacgt
     1501 tggtggcctc gaagtgattg ccgccggagt cgccctggcg gatatcccac agccgcccgg
     1561 cgaactgcac ctggcgcttg atcggcccgt ccggaggtcg gtcggcgccg ctcatactca
     1621 gccgtcctgg ggcaagaaga ggcgccagac gccggtagcg gtggcgcaga gctcgccgcg
     1681 ctcgtcgtag aagcggccct cggcgaaggc catgccgcgt ccgagcttgg tcacgctacc
     1741 gtgcgcctcg aagcggtcgc ccagcgccgc gcgcaggtag ttgatggtca gcgagaccgt
     1801 cacctggccg aactgggtca ggtcgaacgt ctcccaatcg acccgcgcca gcaccgccga
     1861 ggcgatggcc cctcccatgg atccatcgaa catgtacgcg atcacgcccc cgttgagggc
     1921 gtcggtgcct cccccgccgc gttgccaggg cgcggcctgt ggcaagcgca ccacgaactc
     1981 gccgggcgcg cgctccgcga cctccagccg ctgcccttcc acgaacgggc ggaacttctc
     2041 ccggaaccag gcgacggctt cgacgtgggg ctcgctcatg tcgcccgcca gatggtcgtg
     2101 aagggctctg cctcgtgctc cggccgcacg ctcctctcgc cctggagctt ctgcaggtga
     2161 gccagcacgg tggcgccggc cgcgcggcgg aactgcggca gcacgtcggg gtagaggtcg
     2221 atcacgatcg cctcgatggt ggccggctgg cgcgagatgc gctcgtagac ctcgcgctcg
     2281 cggttgagcc ggtgctcgat gtactcgtcg agccgcgccg cgccttcggg gatcgccggg
     2341 ccgtgccccg gcaggatgcg gccgacgccg agcgcccgcg cccgcgccag cgaggcgaga
     2401 tattcgacca tgctgccgtg cggcggaaag ataccgacgg tgccgttgcc cgagacgagg
     2461 tcgccggcca gcagcgtgcc gctgctgggc tcgaagaggg cgatgtggcc cggcgcgtgg
     2521 cctggggtca gcaccgcctc caaccgcccg ccgtcgatct ccagcaccat cccatcggcc
     2581 agctcctggt cgacctcgtc ccaggtcaac gatctcccga ggatcggctg gttgccgggg
     2641 tgcaacagca gcggcacgcc gagcaggcgc ttcagcggca cggcggcctg cgcgtggtcg
     2701 gggtgggcgt gggtcagcac gatggcgcgc accggcaccc cagcgcgcgc ggccagcgcc
     2761 tcttgaatca gtgcgacgcc cggctcggag ccgggatcga cgatcagcaa ctcgcgctcg
     2821 ccgaccaggt aggcgttggt cgattgtccg gccatgaagc caccattggg aacttcgacc
     2881 cgctgtatcg ccaggggcgt tatcccagtc ccagtgcctg tgcctgtgcc tgtacctgtg
     2941 cctgtgcctg tgcctgtctc ggccatctgc tgcgtgccat tccatcgccg ccacgccgga
     3001 gaaccggcct gacgcgagtg tgcgtgcggc tccatcatcc ccgggaaatc tacactgctt
     3061 cggccttcgc cgccacgacg cggcgcgcaa ccagcgcgcc gagccgccgt ttgaaccggg
     3121 gcatggcgcg ctgtgtcgat ttccgcccac tccattcgtc gtattagcga gaccggttct
     3181 gacaccggat ggagcgctgg attccaagga ggaactccga tgaagtacct gctgatgctc
     3241 ttcgatggcg acggcacgag cgcccgccag cagaccgacg acctgccgac gatcatcgcc
     3301 gcgcacgagc gcttcaccga cgagctgcgc gccggcggtc agtacgtcgc ggccggagga
     3361 ttgcagcccc gtgagaccgc gaccaccgtg cgcgtgcgga acggggagac gctgatctcc
     3421 gatggtccct tcgccgaggc gaaagagcag gtcggtggct tctacatggt cgaggccgcc
     3481 gatctcgacg aggcgctggc gctcgccgcg cgggtgccct atacggatac ctacgccgcc
     3541 atcgagatcc ggcccctctt ctgatgacgg gcgagcactc gaccacctcc cggccggctg
     3601 gcgacgccgg ccgggaggat tcaccagcga acccggccgg cgtcgccatc gcggccgtct
     3661 tccgccagga gcacggccgc atcatcgccg gtctgatccg ccgcctaggc gatttcgacc
     3721 gcgccgagga ggcactgcag gatgccttcg ccgccgccct ggagcgctgg ccggacgagg
     3781 gtacaccgcg caaccccgcc gcctggctca ccacgacggc acgacgcaag gcgatcgacc
     3841 gggcacggcg cgagcaactg caggcggcaa agtacgaagc gttcggccgc gagccgggcg
     3901 gagaggcgcg ggatgaggtg gagtatcttg ccgaggagga tgaatccggt tcgattccgg
     3961 acgatcggct gcggctcatc ttcacctgct gccaccccgc gctcgggctg gaggcgcagg
     4021 tcgcgctggc gctgcgcacc ctcggcggcc tgactaccgg cgagatcgcc cgcgccttcc
     4081 tgctgcccga ggcaacggtc gcacagcggc tggtgcgcgc caaacgcaag atccgcga
//
LOCUS       DASUBI010000076         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_147324, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000076 DASUBI010000000
VERSION     DASUBI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147324"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="rsmG"
                     /locus_tag="VFU72_01385"
     CDS             <1..652
                     /gene="rsmG"
                     /locus_tag="VFU72_01385"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5422169.1"
                     /translation="LRRVLLDWNSRVNLTRITDPAEVEIKLFLDAIALIPWIRRQAED
                     TGRDRPARIVDIGAGAGFPGLPLKIVEPEMELTLIEATGKKVEFIESAVAALELPGVR
                     AIHGRAEEMGHDAALRGRFDIATARAVARLPALLELCQPFCRSGGWGLYPKGRAAQAE
                     ADESARALRLLKTTLIGVEPAPLPELAGTSIVVVRQNTPAPRQYPRRAGLPAREPL"
     gene            660..1592
                     /locus_tag="VFU72_01390"
     CDS             660..1592
                     /locus_tag="VFU72_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422170.1"
                     /translation="MTDSAIQTEGLTRRFGPVVAVDDLSLQVPRGSIFGFLGPNGSGK
                     TTTIHLLLGLLEPSSGSARVLGLDPLRHGDEVRAHSGALLEYPGLYERLSAQDNLDFY
                     GRVWRIPPAARAERVKELLGPLDLWERRHEPVGDWSRGMKQKLAVARALFHRPEVAFL
                     DEPTAGLDPVAAASLRDDLERLVSHEGVTVFLTTHNLPEAERLCDLVGVIRVGKLLAV
                     DTPERLRLNAGGQRVEISGAGFSPDLVSQLRARPDVRAVEQEHGRLSISLNGEVSVAP
                     LVRLLVEGGAAVEEVHKGAASLEEAFLSLMEQPA"
     gene            1589..2314
                     /locus_tag="VFU72_01395"
     CDS             1589..2314
                     /locus_tag="VFU72_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422171.1"
                     /translation="MITDSLTVAIKEWKEMLKGGSRGRTASLLLIAIYGIIFPLRVGR
                     PWITGYQSIAFGIFVPFFLVFSVVADAIAGERERHTLETLLASRLSDRAILLGKIAAV
                     TLYGWGLALASLLLGLITVNVTQGGGIELYPAKRAAALVGFSLLVALLAAGIGVLASL
                     RAATVRQAYQTLTVSWLVAFFAAVFGIQQLSARTQHRLLDWLRGVGFGRAALIVAAIV
                     LLIDVLLFALALVRFQRARLILD"
     gene            complement(2526..4184)
                     /locus_tag="VFU72_01400"
     CDS             complement(2526..4184)
                     /locus_tag="VFU72_01400"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU5422172.1"
                     /translation="MELTLSEVLRLARRWWWLIVLCPLLAAGIAYLVSSLLTPVYQAR
                     ATLLVEQSQTPGSQGYQDILASQQLTQTYSRLVSTRPVLDEVARELGGGLTADGLSEH
                     LSVSPLNGTQLVHISVTDTSSERAAEIANTVGQTFIKQRQTQQAALTGSTRDDLQHSV
                     DGLKQQIDDVSARISVLQARSDVASDQVQSQLTVLQRQLNDLQTTYSGLLEVLQRMNL
                     DVAQATTQIQLVEQAVAPLQPVRPRVMLNTAAAGALGLLLALGSVLVIGYLDDTVKSS
                     EDVRRLTGAYALGLVGALRAPAAVEPLAYPHSAGAESYRALRTSLQFATAGRAARVIV
                     VTSAEPGAGKTTTVANLGMVLAQLGQRVVLVDADLRLPRLHTHFAGFTNRSGLTNRLA
                     ADADEDPEAFLQTTAVEGLRVLSAGPLPPNPAELLSGPRLTAVLANLLDYADVILLDV
                     PPLAVSDPLVVAAAADGIVVVARHGRTRNKTLTRTVGELSRVGKPLFGVVVNFAQISG
                     DDAYYYKASYGASEAETVAMAPKRRLRRRFSRGAPSVPISEIKR"
BASE COUNT          690 a         1533 c         1518 g          723 t
ORIGIN      
        1 gctgcgccgg gttctgctcg actggaactc ccgcgtcaac ctgacccgca tcaccgatcc
       61 ggccgaggtc gagatcaagc tctttctcga cgccatcgcg ctcatcccct ggatccggcg
      121 gcaagcggag gataccgggc gtgaccgtcc ggcgcggatc gtcgacatcg gcgcgggcgc
      181 gggttttccc ggcctgccgc tcaagatcgt cgaaccggag atggaactga ccctcatcga
      241 ggccaccggc aagaaggtcg agttcatcga gtccgccgtg gcggcgctgg aactgccggg
      301 agtgcgggcg atccacggac gagccgagga aatgggccac gacgctgccc tacgcggccg
      361 cttcgacatc gccaccgcgc gggccgtcgc gcgcctcccg gcgttgctgg agctgtgtca
      421 gccattctgt cgcagcggcg gctgggggct ctaccccaag gggcgcgcgg cccaggcgga
      481 ggcggacgaa tcggcccgcg cgctacgact gctcaagacg acgctgatcg gcgtcgagcc
      541 ggcgcccctg ccggagctcg ccggtacgtc catagtcgtc gtgcgccaga acacgcccgc
      601 gccacgacag tatccgcggc gtgctggtct gcccgccagg gagccgctgt gagcctgcca
      661 tgaccgactc cgccatccag accgaggggc tgactcgccg cttcggcccg gtcgtcgccg
      721 tggacgatct gagcctccag gtgccgcgcg gctcgatctt cggctttctg ggcccgaacg
      781 gctcgggcaa gacgacgacg atccacctcc tgctgggtct gctggagccg agctccggca
      841 gcgctcgcgt gctggggctc gacccgctgc gccacggtga cgaagtccgc gcccactccg
      901 gcgcgctgct ggagtacccc ggcctctacg aacggctgag cgcgcaggat aacctcgact
      961 tctacggccg cgtctggcgc attccgccgg ctgcgcgagc cgagcgggta aaggagctgc
     1021 tgggtccgct cgatctctgg gagcggcggc acgagccggt cggcgactgg agccggggta
     1081 tgaagcagaa gctggccgtg gcccgcgcgc tctttcatcg tccggaggtc gccttccttg
     1141 acgagccgac cgccggcctc gacccagtgg ctgccgcctc cctgcgtgac gacctggagc
     1201 gactggtcag tcacgagggt gtcaccgtct tcctcaccac ccacaacctg ccggaagccg
     1261 agcgtctctg cgatctggtc ggggtgatcc gggtcggcaa gctgctggcg gtcgatacgc
     1321 ccgagcgtct gcggctgaac gctggaggcc agcgcgtcga gatcagcggt gccggcttca
     1381 gcccggacct ggtcagccag ctgcgcgccc ggccggacgt gcgcgccgtc gaacaggagc
     1441 acggacggct gtcgatcagc ctcaatggcg aggtaagcgt cgcacccttg gtgcggctgc
     1501 tggtcgaagg aggcgccgcg gtcgaggagg ttcacaaggg cgcggccagt ctggaggagg
     1561 cgttcctctc gctgatggag caaccggcat gatcacggac agcctgacgg tggcgatcaa
     1621 ggagtggaag gagatgctca agggcggcag ccggggacgc accgcctcac tgctcctgat
     1681 cgcgatctac ggcatcatct tcccgctgcg ggtcggccgc ccctggatca ccggctacca
     1741 gtcgatcgcc ttcggcatct tcgtaccctt cttcctggtc ttcagcgtgg tggccgacgc
     1801 catcgccggc gagcgcgagc gccacacgtt ggagaccttg ctcgccagcc ggctctccga
     1861 ccgcgccatc ctgctcggga agatcgcggc ggtcacgctc tatggctggg gactggcctt
     1921 ggccagcctg ctgctcggcc tgatcacggt caacgtgacc cagggcggcg gcattgagct
     1981 ctatccggcc aagcgcgcgg ccgcgctggt gggattctcg ctgctggtgg cgctgctggc
     2041 cgccggcatc ggcgtgctgg cctcgctgcg cgccgccacc gtgcgccagg cgtaccagac
     2101 gctgaccgtc agttggctgg tggccttctt cgcggcggtc ttcggcatac agcaactctc
     2161 ggcgcggacg cagcaccggc tgctggactg gctgcgcggc gtcggcttcg gtcgggccgc
     2221 cctcatcgtc gcggccatcg tgctgttgat cgacgtgctc ctgttcgcgc tggcgctggt
     2281 ccgcttccag cgcgcgcggt tgattcttga ttgacgaggc ggccggtcgc gtaggtgccc
     2341 atagagatgg tttggtgccc caagaggacg gatccttcga caggctcagg ataacaaagg
     2401 gaaggggatg gggcgtacga cggcgcgtgt cgctgtcgtc agcccgctac tcacccttgt
     2461 caacccgacc gcagtggagg gatccgtact gacgcggctc gcaacctgcc cagtggtcag
     2521 gccgttcagc gcttgatctc ggagatcggc accgacgggg cgccgcgcga gaaccggcga
     2581 cggaggcgcc gcttcggtgc cattgctacg gtctcggcct ccgatgcgcc ataagaagcc
     2641 ttatagtagt acgcatcgtc gcctgatatc tgcgcgaagt tgacgaccac gccgaagagc
     2701 ggcttgccga cccttgacag ttcccccacc gtgcgcgtca gcgtcttgtt gcgggtgcgg
     2761 ccatggcgcg ccaccacgac gatcccgtcg gcggcggccg cgacgaccag cggatcactg
     2821 accgccagcg gcggaacatc gagcaggatg acatcggcgt aatcgagcag gttcgccagc
     2881 accgcggtca agcgcgggcc actcaacaac tcggccggat tcggtggtag cggcccggcc
     2941 gagagcacgc gcaagccctc gaccgcggtg gtctgcagga acgcctccgg atcctcatcg
     3001 gcgtcggccg cgagccggtt cgtcaggcca ctgcggttgg tgaaaccggc aaaatgcgtg
     3061 tgcagccggg gaaggcgcag gtcggcgtcg accaatacca cgcgctgccc cagttgggcc
     3121 agcaccatcc ccaggttcgc gaccgtggtc gtcttgccgg cgcccggctc ggcactggtc
     3181 accacgatga ctcgcgccgc ccgcccggcc gtcgcgaatt gcaggctggt gcgcagcgcc
     3241 cggtaggact ccgcgcccgc gctatgcggg tacgccagag gctcaaccgc ggccggcgcg
     3301 cgcagggcgc ccaccagccc gagcgcatag gcgcccgtca gccggcgcac atcctctgaa
     3361 ctcttgaccg tgtcatccag gtagccgatg accagcacgc tcccgagcgc cagcagcagg
     3421 ccaagcgcgc ccgccgccgc ggtgttgagc atgacgcgcg gccgcaccgg ctgcagcggc
     3481 gcgacggcct gctccaccag ttgaatctgc gtcgtagcct gcgccacatc gaggttcatg
     3541 cgctgcagca cctcgagcag cccgctgtag gtcgtctgga ggtcgttgag ctgccgttgc
     3601 agcacagtca actgcgactg cacctggtcg ctggccacat cagagcgcgc ctgcaacacg
     3661 ctgatacgtg cggagacgtc gtcgatctgc tgcttcaggc cgtccacgct gtgctgcaaa
     3721 tcgtcgcggg tcgagccggt caacgccgcc tgctgcgtct gacgttgctt gatgaaggtc
     3781 tgaccgacgg tgttggcgat ctcggcggcg cgctccgagc tggtatccgt caccgagata
     3841 tgcacgagtt gcgtgccgtt gagcggggag accgacaaat gctccgagag cccatcggca
     3901 gtcagcccgc caccgagctc gcgagcaacc tcgtcgagca ccgggcgagt ggagaccagc
     3961 cggctgtagg tttgcgtcag ttgttgcgag gccaggatat cctgatagcc ctgcgagcca
     4021 ggcgtctgcg actgctcgac caggagggtc gcccgcgcct gatagactgg tgtgaggaga
     4081 gaactgacga ggtacgcgat gccggcggcg agcagcgggc agaggacgat cagccaccac
     4141 cagcgacgcg cgagccggag gacttcggag agtgtgagtt ccacctctgc ccgcctcccg
     4201 acaccaaccc ctcgaacccg cgaccaccgg ccggtcgtga cggtcacctg ctcacgtggc
     4261 gctcgggccc cacgcgcgcg gcgttcgccg atgatcgtcc tcgcgaacac accgcggccg
     4321 cgaatcgtac tcagccgatt gacacgcctg gagccaagca tacacgccgt acggtcagca
     4381 tgcatggcgc acgcgattgt tcctacgatt gtcctatatt cgggtcggag atcgtcgtga
     4441 gcggagcctt gaggcgcctc gtag
//
LOCUS       DASUBI010000077         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1361375, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000077 DASUBI010000000
VERSION     DASUBI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361375"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VFU72_01405"
     CDS             <1..1188
                     /locus_tag="VFU72_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEU5422173.1"
                     /translation="LSSALGGQGPTRYLVLIQNSEEMRPSGGFPGTVALVTFDHGQLR
                     GYEFHDIYDLNSAYVDSQHTPIPQPWPLSQYAPTSELSMLDATWWPDFPISAATEMRM
                     YASTGWPSISGVVAIDPALVSDLLRLTGPVTVQVDGESRVVTADNLLQEIERQRMLFI
                     SGAKEESAHKEMVALVGEQLIERLHGTDTSTLRQAIHLLSTAATKRDIQAYAADSSVE
                     TLLDQRAWTGKLAPNPAAATIAVAYGNIAFGKSSRLLQTAESLTIEPAANGVQRVTLR
                     LDLHHSGDPGNTDTFYSGVQLWWIGLYLPPGSTIQSASQLVASDPSPPNGGSYLVEVR
                     PGESKQLTVTFEMPAAKQLLLRRQSGLTPVKLSVTTPGCSSPAHRVLDRDQQFDLDSG
                     CAP"
     gene            1289..2038
                     /locus_tag="VFU72_01410"
     CDS             1289..2038
                     /locus_tag="VFU72_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5422174.1"
                     /translation="MGLLGRMNTLIKVKINKLLGAAEDPREVLDYSYERQLKMLQDVR
                     RGIVEVTTSRRRLELQKAKLQEGSDKLDGQARQAVSAGRDDLARMALERRSVLQGQMG
                     DLDGQIQTLDGEQAKLEQAETRLRTKIESFRTRKEVVKAQYSAAEAQVRIGEAVTGLS
                     EEMADVGMALDRAEEKTTALQARAGAIDELIDSGALEDFTSTSTSGSDVLDRELARVT
                     VKDSVDSQLAALKKELGAGDQAKQLGEGQPS"
     gene            2035..2313
                     /locus_tag="VFU72_01415"
     CDS             2035..2313
                     /locus_tag="VFU72_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422175.1"
                     /translation="MIVRIATEGQYELDSSYLDKLNAIDNDIVNAIADGDRARYQKLF
                     NQLLELVRRHGTPVDVDELVESQVVLPPPDTTFDEAEELFVGEGMFTG"
     gene            complement(2335..2910)
                     /locus_tag="VFU72_01420"
     CDS             complement(2335..2910)
                     /locus_tag="VFU72_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422176.1"
                     /translation="MRSFFDALLGRTRPEPPKIERLFAMSTAEVALETQLRLAPGDHA
                     GITFRPVESAYFEQMSTDLQQLLQISAKDTGTEVKTLTDEYGYRWIVLADAQFEDLVA
                     TIHIISETLIEQQFGEQLLAGVFRFSTAEGRPVYWIYNYKRGAFYPFVPEGTGRKRDN
                     AFELRLSSAMEKELPVETELERWYPIWDIPF"
     gene            complement(2907..>3742)
                     /gene="htpX"
                     /locus_tag="VFU72_01425"
     CDS             complement(2907..>3742)
                     /gene="htpX"
                     /locus_tag="VFU72_01425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU5422177.1"
                     /translation="SLLYLAFMAVMVYVGFDWISILVIAGILALVQYYGSAWLALRSM
                     GAVEVSPEQAPELHAIIDRLSQIANIPKPRVAISNTDVPNAFATGRNPKNAVVAVTSG
                     LMQRLDTPEIEAVLAHELSHVRNRDVMVMTLANFFAVVAQLLTRSFMWGGFGGGYGRR
                     NRDEGGSIALVYLASILVWVISFFLIRAISRYREYAADRGSAILTGAPTNLMSALVKI
                     SGVMQRIPERDLREVQTMNAFFIIPAIKGDAMSELFSTHPSLDHRLERLRELSRTMEG
                     L"
BASE COUNT          704 a         1193 c         1213 g          632 t
ORIGIN      
        1 ctcagtagcg cgctcggcgg gcagggaccc acgcgctatc ttgttctgat tcagaactcg
       61 gaagagatgc ggccgagcgg cggcttcccg ggcaccgtgg cgctcgtcac cttcgaccac
      121 gggcaactac gcggttacga gttccacgat atctacgatc tcaatagcgc ctatgtcgac
      181 agccagcaca cgccgatccc gcagccgtgg ccactctcgc agtacgctcc cacgagcgag
      241 ctctcgatgc tcgacgccac ctggtggccg gattttccca tcagcgccgc cacggagatg
      301 cggatgtatg ccagcactgg ctggccgtcg atctcgggtg tcgtcgcgat cgatcccgcg
      361 ctcgtcagcg acctgctccg ccttaccggt ccggtcacgg tgcaagtcga tggcgagtcg
      421 cgcgtggtca ccgcggataa cctgctgcaa gagatcgaac gccagcgcat gcttttcatc
      481 tcgggcgcca aggaggaatc ggcacacaaa gagatggtcg ctctggttgg cgagcagctg
      541 atcgagcgcc tgcacggcac cgatacgtcg acgctgcggc aggcgatcca ccttctgagc
      601 acggccgcca ccaagcgcga tattcaggcc tacgcggctg actcctcggt cgagacgcta
      661 ctcgaccagc gcgcctggac cggcaagctg gcgcccaatc cggccgcggc cacgatcgcg
      721 gttgcctacg gcaacatcgc gtttggcaaa tcgagccggt tgctccagac ggctgagtcg
      781 ctgacgatcg agccggcagc caatggcgtc cagcgcgtga cgttgcggct cgatctgcac
      841 cactccggcg accctggtaa caccgatacc ttctatagcg gcgtacagct ctggtggatc
      901 gggctttatc tgcctccggg gagcacgatt cagagcgcca gtcaactcgt ggcgtccgac
      961 cccagccctc cgaacggcgg cagctacctg gtggaggtgc ggccgggcga gagcaagcaa
     1021 ctgacggtca cgttcgagat gccggctgcc aagcaactgc tcttgcgccg ccagtcgggt
     1081 ctcacgccgg tcaagctgag tgtcacgact cccggctgct cgtccccggc gcaccgggtg
     1141 cttgatcgcg atcagcagtt tgatctggac agcggctgcg cgccctagca cgcgagccga
     1201 taggcttgcg gttccagccg gtgcaatgac ggctggtacg cggctataat gtgtctgcag
     1261 gctcgctgcg cagtagatag gatgggcgat ggggctgctc ggccgaatga acacactgat
     1321 caaagtcaag atcaacaagt tgctcggcgc cgccgaggat ccgcgtgagg tgttggacta
     1381 ctcctacgag cggcagctca agatgctgca ggacgtgcgt cggggtatcg tcgaggtgac
     1441 cacctcgcgt cgccgcctgg agctacagaa ggccaagctg caggaaggct ccgacaagct
     1501 cgatggtcaa gctcgccagg cggttagcgc cgggcgcgac gacctggcgc gcatggcgct
     1561 ggagcgtcgc tcggtactgc agggccagat gggcgatctc gacggccaga ttcagacgct
     1621 cgacggggag caggccaagc tcgagcaggc cgagactcgt ttgcgcacca agatcgagtc
     1681 cttccgcacg cgcaaggaag tcgtcaaggc gcagtactcg gcggccgagg cgcaggtgcg
     1741 catcggcgag gccgtcaccg ggctttccga ggagatggcc gacgtcggca tggcgctcga
     1801 ccgggccgag gagaagacga ccgcgctgca ggcgcgcgcc ggcgccatcg acgagctgat
     1861 cgactcgggc gcgctcgagg atttcacctc caccagtacc agcggcagcg acgtgctcga
     1921 ccgtgaactg gcgcgcgtga ccgtcaagga cagtgtcgac tctcaactgg cggcgctcaa
     1981 gaaagaactg ggcgccggcg accaggccaa gcagttgggg gagggtcagc cgtcatgatc
     2041 gttcggatcg cgaccgaggg ccagtacgag ctcgacagct cgtatctcga taagctcaac
     2101 gccatcgaca acgacatcgt gaatgccatc gccgatggtg accgggcgcg ttaccagaag
     2161 ctcttcaacc agttgctgga gctggtgcgc cggcacggta cgccggtcga tgtcgacgaa
     2221 ctggtggagt cgcaggtcgt cctgccaccg cccgacacca cctttgatga ggccgaagag
     2281 ctgttcgtcg gcgagggtat gttcacgggc tagcgctcgc ggcgccaaga gtatttagaa
     2341 cgggatatcc cagatcgggt accagcgctc aagctcggtc tcgactggga gttccttttc
     2401 catcgcgctg gagagacgaa gctcgaaggc gttatcgcgc ttacgtccgg tgccctccgg
     2461 cacgaaaggg tagaacgcgc cgcgcttgta gttgtaaatc cagtagaccg gcctgccttc
     2521 ggccgtgctg aagcggaaga cgccagccag cagttgctcg ccgaactgct gctcgatcag
     2581 cgtctcactg atgatgtgga tggtcgcgac cagatcctcg aactgggcat cggccagcac
     2641 gatccagcga taaccgtact cgtcggtcag cgtcttgacc tcggtgccgg tgtccttggc
     2701 gctgatctgc agcaactgct gcagatcggt gctcatctgt tcgaagtagg ccgattccac
     2761 cgggcggaag gtgatgccgg cgtggtcgcc cggagccagc cgaagctggg tctccaaagc
     2821 gacctccgcc gtcgacatcg cgaataggcg ctcgatcttc ggcggttccg ggcgcgtacg
     2881 ccccagcagc gcgtcgaaga acgatctcac agcccctcca tcgtccgcga gagctcccgc
     2941 agccgctcca ggcggtgatc cagcgagggg tgggtcgaga acagctccga catcgcgtca
     3001 cccttgatcg ccggaatgat gaagaaggcg ttcatcgtct gcacttcgcg caggtcacgc
     3061 tcggggatgc gctgcatcac gccgctgatc ttgaccagcg ccgacatcag gttggtcggt
     3121 gctccggtca gaatcgccga gccgcggtcc gcggcgtatt cgcggtaacg cgagatggcg
     3181 cggatcagga agaagctgat gacccagacc aggatcgatg ccaggtagac cagcgcgatc
     3241 gagccgcctt catcgcggtt gcgccggccg tagccgccgc cgaagccgcc ccacatgaac
     3301 gagcgggtca acaattgcgc caccacggcg aagaagttgg ccaacgtcat gaccatcacg
     3361 tcgcggttgc gcacgtgcga gagctcatgc gccagcacgg cctcgatctc gggcgtatcc
     3421 agccgctgca tcaagccgct cgtcaccgcc acgaccgcat tcttggggtt gcgcccggtg
     3481 gcgaaggcat tgggcacgtc ggtgttcgag atcgcgacgc gcggcttggg gatattcgcg
     3541 atctgcgaca gtcgatcgat gatcgcgtgc aactcggggg cttgctccgg gctgacttcg
     3601 accgcgccca tgctgcgcag cgccaaccag gccgagccgt agtactgtac cagggccaga
     3661 ataccggcga tgaccaggat cgagatccag tcgaagccga catagaccat caccgccatg
     3721 aacgcgagat agaggagcga ca
//
LOCUS       DASUBI010000078         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_324935, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000078 DASUBI010000000
VERSION     DASUBI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324935"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1504)
                     /locus_tag="VFU72_01430"
     CDS             complement(77..1504)
                     /locus_tag="VFU72_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422178.1"
                     /translation="MDVDTALPSPAPTATTSEPAPRRPLSLAARSAITTGFVLAAFLG
                     LVGVVMYVAKKQSALNTLQDRLRDAAITDIITHTDANRDGRLLPPDSPPQKFSQPGSG
                     LYAVILGPDGYHWESTSAIGHDFRFLKPLPTGEQVFTGPVETRMGRLYYYAIGVAFDF
                     PGRKSTPATIMVAQTEQQLEGENAVYRRTLALWLSVLGVMLIVLQLLLLRWSLTPLRK
                     VASEMSQVERGDSDHLDSQYPLELTGLTERINAFIDSEREQRTRYRHTLADLAHSLKT
                     PLAVIRSQLESASDESTRRGVLDQVRRMDELVAYQLARAATSGRQTFAAAVPIASHAE
                     DLVQSLEKVYAAKNVLCEFDIADGASFHGELNDLLELMGNLLENAFKWTRHHVVLVVQ
                     KQPQPGRGRPGLLISVEDDGPGIAADKIEKVLQRGVRGDERVQGHGIGLSIVQDIVRA
                     YRGELTVDHSPDLGGARFSVKLPPE"
     gene            complement(1452..2144)
                     /locus_tag="VFU72_01435"
     CDS             complement(1452..2144)
                     /locus_tag="VFU72_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422179.1"
                     /translation="MRILLVEDEAPLRETLAARLKRDGFAVDAAQDGEEALYLGREVP
                     FDLAIIDLGLPKMSGMELIKALREHGQRYPVLILTARGGWQDKVEGLKQGADDYLVKP
                     FHVEELLARINALVRRASGWSKPVLACGPIKLDTTAQTVTVEGKIVDLTSYEYKVLEY
                     LMLHAGELVSKADLTEHIYQQDFDRDSNVLEVFIGRLRRKLDPEGTLKPIETVRGRGY
                     RFAIPRTDGDDE"
     gene            complement(2144..2473)
                     /locus_tag="VFU72_01440"
     CDS             complement(2144..2473)
                     /locus_tag="VFU72_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU5422180.1"
                     /translation="MKAHRILPLFAPLALTLTLGASPAAWAQAKPKDLSLGEAVSRAQ
                     QETNGQVLSADSVRRDRRTEYRVKVLTPQGHVRVMTIPASGDNARPPSPSTRNPPARR
                     PGGKEKH"
     gene            complement(2556..>2856)
                     /locus_tag="VFU72_01445"
     CDS             complement(2556..>2856)
                     /locus_tag="VFU72_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEU5422181.1"
                     /translation="VMLGRAAYHDPYLLARLEHALYGTPLPGRDDVLRRLRPYIEAEL
                     KRGTALKHITRHLLGLYQGEPGARGFRRVLSEGAHLPGAGWALIERALEPLAATA"
BASE COUNT          390 a          948 c         1036 g          482 t
ORIGIN      
        1 taggtcgtgc gcgatggccc tggcgttgac cagagccaaa acctgtagcc aaagcattcg
       61 cgcacagggt gcgctcctac tctggcggta atttcacgct gaagcgcgcc ccgcccaggt
      121 cgggcgaatg atccaccgtc aactcgccgc ggtaggcgcg cacgatgtcc tgcacgatgg
      181 acaggccgat gccgtgcccc tgcacgcgct cgtcgccgcg cacgccgcgc tgcagcacct
      241 tctcgatctt gtccgcggcg atgcccgggc cgtcgtcttc cacgctgatc agcaggccgg
      301 ggcggccgcg gccgggctgc ggctgcttct gcacgaccag caccacgtgg tggcgcgtcc
      361 acttgaacgc gttctccagc aggttgccca tcagttcgag cagatcgttc agctcgccgt
      421 ggaaggaggc gccatccgcg atgtcgaatt cgcacagcac gttcttcgcg gcatagacct
      481 tctccaggct ctgcaccagg tcctcggcgt ggctggcgat cggcaccgcg gcggcgaagg
      541 tctggcggcc cgaagtggcg gcgcgcgcca gctggtaggc gaccagctcg tccatgcggc
      601 gcacctggtc gagcacgccg cgccgggtgg attcgtcgct ggccgattcc agctgcgagc
      661 ggatcaccgc cagcggcgtc ttcaggctgt gcgcgaggtc ggcgagggtg tggcggtagc
      721 gcgtgcgctg ttcgcgctcg ctgtcgatga aggcgttgat gcgctcggtg aggccggtca
      781 gctccagcgg gtactggctg tccaggtggt cgctgtcgcc gcgctcgacc tggctcatct
      841 cgctggccac cttgcgcagc ggggtgaggc tccagcgcag cagcagcagc tgcagcacga
      901 tcagcatcac gcccagcacc gacagccaca gcgccagcgt gcgccggtac accgcgttct
      961 cgccttcgag ctgctgctcg gtctgcgcca ccatgatggt ggcgggggtg gacttgcggc
     1021 ccgggaagtc gaacgccacg ccgatcgcgt agtagtagag ccggcccatg cgggtctcga
     1081 cggggccggt gaatacctgc tcgcccgtcg gcagcggctt gaggaagcgg aagtcgtggc
     1141 cgatcgccga ggtggattcc cagtggtagc cgtccggccc gaggatcacc gcgtacaggc
     1201 cggagccggg ctgggagaac ttctgcggcg gcgagtcggg cggcagcagg cggccgtcgc
     1261 ggttggcgtc ggtgtgggtg atgatgtcgg tgatcgcggc gtcgcgcagc cggtcctgca
     1321 gtgtgttcag tgcactctgc ttcttggcca cgtacatgac cacgccgacc aggccgagga
     1381 aggcggccag cacgaaaccg gtggtgatgg ccgagcgcgc cgccagcgac agcgggcggc
     1441 gcggcgcagg ctcactcgtc gtcgccgtcg gtgcggggga tggcaaagcg gtatccacgt
     1501 ccacgcacgg tctcgatggg cttgagggtg ccttccggat ccagcttgcg gcgcaggcgg
     1561 ccgatgaaga cttcgagcac gttggagtcg cggtcgaagt cctgctggta gatgtgctcg
     1621 gtgaggtcgg ccttggagac cagctcgccc gcgtgcagca tcaggtattc cagcaccttg
     1681 tactcgtagc tggtgaggtc gacgatcttg ccttccaccg tcaccgtctg cgcggtggtg
     1741 tcgagcttga tcgggccgca ggccagcacc ggcttggacc agccgctggc gcggcgcacc
     1801 agcgcgttga tgcgcgccag cagttcctcg acgtggaacg gcttgaccag gtagtcgtcg
     1861 gcgccctgct tgaggccttc caccttgtcc tgccagccgc cgcgcgcggt gaggatcagc
     1921 accgggtagc gctggccgtg ctcgcgcagc gccttgatca gctccatgcc ggacatcttc
     1981 ggcaggccca ggtcgatgat ggcgagatcg aacggcacct cgcggccgag gtagagcgcc
     2041 tcttcgccgt cctgcgccgc gtcgacggcg aagccgtcgc gcttcagtcg tgccgccagc
     2101 gtctcgcgca gcggggcctc gtcttccacc aagaggatgc gcatcagtgt ttctccttgc
     2161 cgccgggacg ccgggcgggc gggttgcggg ttgacgggga gggtggccgg gcattgtcgc
     2221 cgctggcggg gatcgtcatc acccgcacgt ggccctgcgg cgtgagcacc ttgacgcggt
     2281 actcggtgcg gcggtcccgg cgcaccgagt cggccgacag aacctggccg ttggtttcct
     2341 gctgtgcccg gctcacggcc tcgccgaggc tgaggtcctt gggcttggcc tgcgcccatg
     2401 ccgccgggct cgcgcccagc gtcagcgtca gcgccagcgg cgcgaacagc gggaggattc
     2461 gatgggcctt catagccatg cttcgaccac gatctgtttc tggccgttca gtttgggcga
     2521 gcagggctga atagtgaccg gacgggcgac cgctgtcacg cggtcgccgc cagcggctcc
     2581 agtgcccgct cgatcagcgc ccagcccgcg cccggcaggt gcgcgccctc gctgagcacg
     2641 cggcggaagc cgcgcgcgcc cggttcgccc tggtacaggc cgagcaggtg tcgggtgatg
     2701 tgcttgagcg cggtgccgcg cttgagctcg gcctcgatgt aggggcgcag gcgccgcagc
     2761 acgtcgtccc ggccgggcag cggggtgccg tacagcgcgt gttcgagccg ggcgagcagg
     2821 tacggatcgt ggtaggcggc gcggcccagc atcacg
//
LOCUS       DASUBI010000079         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_965604, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000079 DASUBI010000000
VERSION     DASUBI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965604"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /gene="mnmE"
                     /locus_tag="VFU72_01450"
     CDS             <1..1148
                     /gene="mnmE"
                     /locus_tag="VFU72_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU5422182.1"
                     /translation="DVVEISCHGGPVPLREVLRLALAAGARQAEAGEFTLRAFLNGRV
                     DLAQAEAVMAVVSARTPESLTLAVEELRGRLGGRLAPARAALLSALAYLDASADFPED
                     EVPPLDLARTLRHAAAALEEVLAGARLGRLFREGVEIAIVGRPNVGKSSLLNALLGAE
                     RAIVTEIAGTTRDVISETITLRGIPATLLDTAGIAETEDIVERIGIDRSRRAIVSAGL
                     AVLVLDGSQPPTEADRAVVRLLADRLSRDGAGSERLVVALNKRDLPERHDHAELLALL
                     PGCPAIALSTVSGAGLPQLEETLYARLQAQAGEGREPALVTLRQQQALARALESTRSA
                     LVELSDVPLDLVAVDVRDALLAVGEITGEHIGERVLDEIFSRFCIGK"
     gene            1184..1789
                     /locus_tag="VFU72_01455"
     CDS             1184..1789
                     /locus_tag="VFU72_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEU5422183.1"
                     /translation="MAEHERGAPPPDRPLPPVPPEAIRQNSLSTVLTPTGRLRKRLSL
                     KSELTLAALPSATVLVMFLIIDRFSTRHLLFTSLASSAFLIYLDPLHAMNNVRTLIMA
                     HVGAALFGIGSWALFGHDYSAGIAAMFVTILYMIVLDVMHPPAASTALLFALGGDSQN
                     QFLLFLLALGITVVLVILQRSAVWLFTRLTDEEPPEQAPGS"
     gene            complement(1786..2250)
                     /locus_tag="VFU72_01460"
     CDS             complement(1786..2250)
                     /locus_tag="VFU72_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU5422184.1"
                     /translation="MQLNSDAFQNGQRLPNEFTCDGPGVSPPLYWLEPPTGTHAFALI
                     ADDPDARRGVFTHWLIYNIPGDRTQLQLAVPQVETMTTPTLQGVNDFGTIGYGAPCPP
                     PGDPAHHYTFTLYALDAPLDLRGGEVRRQVEQAMQGRVLGEARLVGLFSRPG"
     gene            2387..>2693
                     /locus_tag="VFU72_01465"
     CDS             2387..>2693
                     /locus_tag="VFU72_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEU5422185.1"
                     /translation="MSEAASELHDGMAVVDSDGRGFGAVAQVRRDDFLVHRELELDVY
                     VLRFAVRAVDGDTVVLKAPGDRLQALDWAVPAVHGLQQTGTAPVVETVTDAADLSWPR
                     "
BASE COUNT          380 a          952 c          950 g          411 t
ORIGIN      
        1 aggacgtggt cgagatctcc tgccatggcg ggccggtgcc gctacgcgag gtgctgcggt
       61 tggccctggc cgccggcgcc cgccaggccg aggcgggcga gttcaccctg cgcgccttcc
      121 tcaatggacg cgtcgatctg gcgcaggcgg aggcggtcat ggcggtcgtc tcggcgcgca
      181 cgccggagtc actgacgctg gcggtcgagg agctgcgcgg ccggctcggc ggccgcctgg
      241 cgccggcacg cgcggcgctg ctctcggcgc tggcctacct cgacgcctcg gccgacttcc
      301 cggaagacga ggtgccgccg ctcgacctgg cgcggacgct gcggcacgcg gcggcggcgc
      361 tcgaagaggt actcgcgggc gcacggctgg gccggctctt ccgcgagggg gtcgagatcg
      421 ccatcgttgg gcggccgaat gtgggcaagt cgagcctctt gaacgcgctc ttgggcgccg
      481 agcgcgccat cgtcaccgag atcgccggca ccacccgcga cgtgatcagc gagacgatca
      541 cgctgcgcgg catcccggct accctgctcg acaccgccgg catcgccgag accgaggaca
      601 tcgtcgagcg catcggcatc gaccgttcgc gccgcgccat cgtgtcggcc ggactggcgg
      661 tcctggtgct cgacggcagc cagccaccca ccgaggccga ccgcgcggtc gtgcgcctgc
      721 tagctgaccg gctcagccgc gacggcgcgg gctccgagcg gctggtggtc gcgctcaaca
      781 agcgtgacct gcccgagcgg cacgaccacg ccgagctgct ggcgctcctg ccgggctgcc
      841 cggcgatcgc gctttcgacc gtcagcggcg cgggactgcc gcagcttgaa gaaacgctct
      901 acgcgcgcct gcaggcgcaa gcaggcgaag gtcgcgagcc ggcattggtt accctgcgcc
      961 agcagcaggc gctcgcccgc gcgctggaga gcacgcgttc tgccctggtg gaactgagcg
     1021 acgtgccgct cgatctggtg gcggtcgatg tgcgcgatgc gctgctggcg gtgggggaga
     1081 tcaccggcga acacatcggc gaacgcgtgc tcgacgagat cttcagccgc ttctgtatcg
     1141 gcaagtagcg cctcgggcgc gcggagagga ggccggggcc gcgatggccg agcacgagcg
     1201 cggtgcccct ccccccgatc gtccgctgcc tcctgtaccg cccgaggcga tccgccagaa
     1261 cagcctgagc accgtactca cgcccactgg gcggctgcgc aagcggctca gcttgaagtc
     1321 ggagctgacc ctggctgccc tgccaagcgc gacggtgctg gtgatgttcc tgatcatcga
     1381 ccgcttcagc acccgccacc tcctctttac ctcgctcgcc tccagcgcct tcctgatcta
     1441 tctcgacccg ctgcacgcca tgaacaacgt gcgcacgctg atcatggcgc acgtcggagc
     1501 ggccctcttc ggtatcggta gttgggcgct cttcggccat gactactcgg ccggcatcgc
     1561 ggcgatgttc gtcaccatcc tctacatgat cgtgctggac gtgatgcacc cgcccgccgc
     1621 ctccaccgcg ctcctgttcg cgctcggcgg cgacagccag aatcagttcc tgctcttcct
     1681 gctggccctg ggcatcaccg tagtgctggt catcctgcag cgctccgcgg tctggctctt
     1741 cacgcgcctg accgacgagg agccacccga gcaggcgccg ggcagctagc ccggccgcga
     1801 gaagaggcca acgagccgcg cctctcccag cacgcgcccc tgcatcgcct gctccacctg
     1861 ccggcggacc tcgccgccgc gcaggtcaag cggcgcgtcg agcgcgtaga gggtgaaggt
     1921 gtagtggtgt gccgggtcgc cgggcggcgg gcagggcgca ccatagccga tcgtgccgaa
     1981 gtcgttgacc ccctgcaacg tcggggtggt catggtctcc acctgcggca ctgccaactg
     2041 cagttgagtg cggtcgccgg ggatgttgta gatcagccag tgggtgaaga cgccgcgccg
     2101 cgcatcgggg tcgtcggcga tcagggcgaa ggcgtgcgtg cccgtcggcg gttcgagcca
     2161 gtagagcggc ggcgagacgc cgggcccatc gcaggtgaat tcgttcggca ggcgctgtcc
     2221 attctgaaat gcatcgctgt tcagctgcac gcaccctctc ctctcatagt cggcatggcg
     2281 ccagcgcggc gcaccgctcc cccgcacctc gcgcaccaac gaccacgccg cagggcacgg
     2341 gctttgcagc cgggcaacga ttgacgctcg gcgagaggag aacacgatga gcgaggcagc
     2401 cagcgaactc cacgacggta tggcggtggt cgactccgac ggcaggggat ttggcgcggt
     2461 ggcgcaggtg cgccgggacg attttctggt ccaccgcgaa ctggagctgg acgtctacgt
     2521 gctgcggttc gcggtgcggg cggtcgacgg cgacacggtc gtgctcaagg cgcccggcga
     2581 ccgtctgcag gcgctcgact gggccgtgcc ggccgtccac ggactgcagc agaccggcac
     2641 cgcgccggtt gtggagacgg tgaccgacgc cgcggacctg agctggcccc gtg
//
LOCUS       DASUBI010000080         6606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_556284, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000080 DASUBI010000000
VERSION     DASUBI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556284"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFU72_01470"
     CDS             <1..576
                     /locus_tag="VFU72_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5422186.1"
                     /translation="VASGVRRVEAVTGHASAERALRLEEATAELARGLHVPEEAITEQ
                     VFDLAERLRARERELERLQVELANSRLGDVLGSATSVDGVRVLATQVEAPGREALLQM
                     GDRLRDRLGSGVVVLGSVIAEEPVLIAMVTRDLVGRGLHAGTIIKEILPLVGGRGGGR
                     AESAQGGGGEPGKLGAALAAVPGAVERQVAG"
     gene            623..2350
                     /locus_tag="VFU72_01475"
     CDS             623..2350
                     /locus_tag="VFU72_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422187.1"
                     /translation="MSTRETTVVHVASEAEFDAFVEHLRGLRHEHILITLEETSDVLL
                     TGVEYRRAASIARLGEVTLTIATTDPLRQQLASMLGWRVETPPKPAIEPEPVVDQSTT
                     ADLATYRPEHLPAGEESAGAAAGPGTRTETEEISLDESAPHPPPTPEPAVHHRAAPGA
                     ATAEVAVPPREPRTRPRPAGSRPPDRRRRRRGLQMLIVGIPLLLLIAAGSAGAAYYYV
                     PTAIVQLTPAERTISVDLVYGLARPDTKYDLTAEPTPVSHTVSFTKSSATTGKRTEPD
                     ATAGGTLQLLNPTMAVITVAGGTTLKASNGVDYVIDADVAVPAADPFGTSTLGSAIVK
                     IHAANPGAGGNLAAGGLAGQLKSGVYFKNANAVSGGTDKTIAVVTQADLDALNAAAVD
                     DLKANSEPQFKQSLDPNVQLVPNSEQVGEPQISFSHKVGEDADQVAVTASQTVSGQVF
                     DPTKLTTEARDEVGRRLTQQVGNDVVLLGDTLTIGQPKPLEDGTSYTIRAEGRVRQII
                     SAQTQESLPGELAGKSLADAESIVNGVPGAATGKITIKPRWLPGRLPQLAKNIRIVVG
                     GSTSGTTGP"
     gene            2355..2753
                     /gene="ruvX"
                     /locus_tag="VFU72_01480"
     CDS             2355..2753
                     /gene="ruvX"
                     /locus_tag="VFU72_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5422188.1"
                     /translation="MALDVGARRVGVAVSDELGLIASPVGAVDLKRRGFERLQELIAH
                     YRPARLIVGLPKNMRGGEGQQAAETRVFADNLEAATGVPVTYWDERLTTFMAEQALIE
                     SGRKRKERKERVDAVAAALILQNYLDAQAR"
     gene            2849..3133
                     /locus_tag="VFU72_01485"
     CDS             2849..3133
                     /locus_tag="VFU72_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU5422189.1"
                     /translation="MKLIIAVVQNEDADNVIDALLENDFRATRLASTGGFLRRGNTTV
                     MIGVQDHQVDQVLGLIRQEARARAQSEAPGAVQTAAATVFVLDLEDYQRL"
     gene            3169..4308
                     /locus_tag="VFU72_01490"
     CDS             3169..4308
                     /locus_tag="VFU72_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5422190.1"
                     /translation="MHTRDTLAFQWRGGRVSVRLTWLLAVTVVTGFTLARIVPEMSRW
                     QERIFWYLGVVVVISGAIMLLLLHEAAHDWMARRLGIGVSPSRLYPFGSVSEGYADPG
                     TPRSELLIALAGPLANLLVALVLGGGWFLLRAGDLHRTLLGLLALASAVIGAANLLPG
                     LPLDGGRILRALVWYLHQDYNSGTRAAVFYGQVISTFTLAAGVIALGSHTSWQPLGAW
                     IILLSWALNRTGREEMLRSELVSRGARVSVGDVVVGLNARIHPEQTLEDVLDVLLAHG
                     QLGPALVSDEGRVSGILSLDQLRRWPRAGWAAHTVAEAMIPLARVASISADASVRELL
                     DRLVDGQVEVLAVVKQGEVLGAVDRRLAARQLFERSRAETQHKAR"
     gene            4467..5744
                     /gene="hflX"
                     /locus_tag="VFU72_01495"
     CDS             4467..5744
                     /gene="hflX"
                     /locus_tag="VFU72_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5422191.1"
                     /translation="MAVDWQRNGWDIGASLEELARLAETADLEVADAVTQQLAHPHAG
                     HYLGSGKLDEIVSRCQEGDFDVVLTNDELAPAQLRSLEDALQLKVIDRTALILDIFAG
                     RARTHEGRLQVELAQLEYRLPRLTRMWTHLSRQAVGGVGLRGPGETQLEVDRREARRR
                     IAWIKEQLAEVHQHRELYRERRRRGKIPVVSLVGYTNAGKSTLLNALAGPSTLAEDKL
                     FATLDPTTRRISLPSGREVLLTDTVGFIQNLPTFLVAAFRATLEEILEASILVHVVDS
                     THPMAEQQGATVNAVLEELGADSRPTITALNKIDAVSEMANGSGSVLPFASAEHLVGI
                     SAQTGEGLDGLLRQIEVLLEAEQQMVDVRLLIPFHQARLVDLFHRRGKVEREDFNEEG
                     TVLDGRLPAHLLPAFADYRAGPVQDGSPGAAGR"
     gene            5854..6219
                     /locus_tag="VFU72_01500"
     CDS             5854..6219
                     /locus_tag="VFU72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5422192.1"
                     /translation="MAGTLTDVLQGLRQGGGFDMVAVVGADGLPIDSCNAPDMDPDSL
                     AALAASGLLMMDAIGQELRQGKATQAILEFERSLVVLEPVQHDLVLMAVANGESNLGR
                     VRLVLRRALPEVAQAISAF"
     gene            6234..>6606
                     /locus_tag="VFU72_01505"
     CDS             6234..>6606
                     /locus_tag="VFU72_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5422193.1"
                     /translation="MSQPARPGSYQSAGVDIERAERAKARIAARVAALANPNVIGGIG
                     HFGGFFRAPRSDKPLVLVSTADSVGTKVMLAAATGKHYGIGVDLVNHCVNDLLATGAR
                     PLFFLDYYATPELDPEALAEIV"
BASE COUNT         1058 a         2287 c         2247 g         1014 t
ORIGIN      
        1 gtcgccagcg gcgtgcgtcg cgtcgaggcg gtcaccggcc acgccagcgc cgagcgcgcg
       61 ctgcggctgg aggaggcgac cgccgagctg gctcgtggcc tgcacgtgcc ggaggaggcg
      121 atcacggagc aggtcttcga cctggcggag cggctgcggg cgcgcgagcg cgagcttgag
      181 cgactgcagg tggaactggc caactcacgg ctgggggatg tgctgggcag cgccacctcg
      241 gtcgacggag tccgcgtgct ggcgacacag gtcgaggcgc cggggcgcga agcgttgctg
      301 cagatgggcg atcgcctgcg cgaccggctc ggctcgggcg tcgtggtact tgggtcggtt
      361 atcgcagaag agccggtgct gatcgccatg gtcacgcgcg atctagtcgg gcgtgggctg
      421 cacgccggca cgattatcaa agagattctg ccgctggtgg gcggtcgcgg cgggggacgc
      481 gccgagtcgg cccagggcgg tggcggagag ccgggcaagt tgggggccgc gctggcggcc
      541 gtgcccggcg cggtggagcg acaggtcgcg ggctagacgc cggcttgccc cgaccacgcc
      601 agtaatcgag ggaagggcaa gcatgagcac gcgtgagacg accgtcgtgc acgtcgccag
      661 cgaggcggag ttcgacgcct tcgtggagca tctgcgcggg ctgcggcatg agcacatcct
      721 gatcacgctg gaggaaacca gcgacgtact cttgaccggc gtggagtatc gccgtgccgc
      781 cagcatcgct cgcctgggcg aggttacgtt gaccatcgcc accaccgatc cgctgcgcca
      841 gcagctcgcc tcgatgctcg gctggcgggt ggagaccccg cccaagcccg cgatcgagcc
      901 tgagccggtc gtcgatcagt ccaccaccgc ggacctggcg acctatcgcc ccgagcatct
      961 ccccgccggc gaggagagcg cgggcgccgc ggccgggccc ggtacgcgca ccgagaccga
     1021 ggagatcagc ctggatgagt cggcgcctca tccgccgccc acacccgagc cggcggtcca
     1081 tcaccgcgcc gcccctggag ccgcgacggc agaggtcgcc gtgcccccac gggagccgcg
     1141 cacacgcccg cgcccggcag ggagccgccc gccggaccgc aggcggcgcc ggcgcggcct
     1201 ccagatgctg atcgtcggta tcccgctcct gctcctgatc gccgccggca gcgccggtgc
     1261 cgcctactac tacgtgccga cggcgatcgt gcaactgacg ccggccgagc gcaccatcag
     1321 cgttgacctg gtctatggcc tggcgcgacc cgacacgaag tacgatctta ccgccgagcc
     1381 gacgccggtc tcccacaccg tgtccttcac caagtccagc gccacgaccg gcaagcgcac
     1441 cgagcccgac gccaccgccg gcggcacgct ccagctcctc aacccgacga tggctgtgat
     1501 caccgtagcc ggcggcacga cgctgaaggc ttctaacggc gtcgactacg tcatcgacgc
     1561 cgatgtcgcc gtccccgccg ccgatccctt cggcaccagt acgctgggca gcgccatcgt
     1621 caagattcac gcggccaacc ccggcgcggg cggcaatcta gcggccggag gcttggcggg
     1681 gcagctcaaa tcgggcgtct acttcaagaa cgccaacgcc gtctcgggtg gcaccgacaa
     1741 gaccatcgcg gtcgtgaccc aggccgacct cgacgcgctg aatgccgctg ccgtggacga
     1801 cctgaaagcc aattccgagc cgcagttcaa gcaatcactc gaccccaacg tgcagttggt
     1861 gccgaactcc gagcaggtcg gcgagccgca gatcagtttc tcccacaagg tgggcgaaga
     1921 tgccgatcag gtcgccgtca ccgccagtca gacggtcagc ggccaggtct tcgatcccac
     1981 caagctgacg acggaggcgc gcgacgaggt tgggcggcgc ctgacgcagc aagtcggcaa
     2041 cgacgtggtg ctcctcggcg ataccttgac gatcggccaa ccgaagccgc tggaggacgg
     2101 cacctcgtat accatccgcg ccgaggggcg tgtacgccag atcattagcg cccagacgca
     2161 ggagtcgctg cccggggagc tcgccggcaa gagccttgcg gatgccgagt cgatcgtgaa
     2221 cggtgttccc ggcgcggcca caggcaagat cacgatcaaa ccgcgctggc tgccggggcg
     2281 cctgcctcag ctcgccaaga acatcaggat cgtggtcggt ggaagcacca gcggtacaac
     2341 cgggccgtag attgatggcg ctcgacgtcg gggcgcgacg agtgggggtg gcagtcagcg
     2401 atgagcttgg cctgatcgcc agccctgtcg gcgcggtcga cctcaagcga cgcggattcg
     2461 agcggctcca ggagctgatc gcgcactacc ggcctgcccg tttgattgtc ggcctgccca
     2521 agaatatgcg cggcggcgag gggcagcagg ccgccgaaac tcgcgtcttc gccgacaatc
     2581 tcgaagccgc caccggcgtg ccggtcacct actgggacga gcgcctgacg accttcatgg
     2641 ccgaacaggc gctgatcgag tccggtcgca aacgcaaaga gcgcaaggag cgcgtcgacg
     2701 ccgtggcggc cgccctgatt ttgcagaact acctcgatgc gcaggcgcgt tgacgccgcc
     2761 gtcgccctgt cgcggttgac ggtcgcgcgg cagcgtctat aatcccgcta atcccgatgt
     2821 gtgagccggg gaacgaggag agggatgcat gaagctgatc atcgcggtcg tgcagaatga
     2881 ggacgcggac aacgtgatcg acgcgctact cgaaaatgac ttccgcgcga cgcgcctcgc
     2941 cagcaccggc ggattcctgc ggcgcggcaa tacgaccgtc atgattggcg tgcaagacca
     3001 tcaggtcgac caggtgctgg ggctgattcg ccaggaagca cgggcccggg cgcagtccga
     3061 ggctccgggc gcggtgcaga ctgccgccgc caccgtcttc gtcctcgatc tcgaagacta
     3121 ccagcgcttg tagtccacca cacaccaccg cgcgcgtctg gagccagcat gcacacccgc
     3181 gatactctgg cgttccagtg gcgaggcggc cgggtttcag ttcggctcac ctggctactg
     3241 gcggtaacgg tcgtcaccgg cttcaccctg gcacgcatcg tccccgagat gtctcgctgg
     3301 caagagcgca tcttttggta cctcggggta gtcgtcgtca tcagcggtgc gatcatgctg
     3361 ctcctgctgc acgaggccgc ccacgactgg atggcccgac gtctcggcat cggggtctcg
     3421 ccctcgcgcc tgtacccgtt cggcagcgtc tccgagggct atgctgaccc cggcaccccg
     3481 cgcagcgagc tgctcatcgc gctggctggc ccgctggcca atctcctggt cgcgctcgtc
     3541 ctcggcggcg gctggttcct gttgcgggcc ggcgatctcc atcgcacgct gctcggtctg
     3601 ctggcgctgg ccagcgccgt catcggcgcg gccaacctgc tcccgggcct gcccttggat
     3661 ggcgggcgca tcttgcgcgc gctcgtctgg tacctgcacc aggactacaa ctccggcacg
     3721 cgcgcggcgg tcttctacgg tcaggtgatt agcaccttca ctctggcggc gggcgtgatc
     3781 gcgctcggca gtcacacgag ctggcagccg ttgggcgcct ggatcatcct gctcagttgg
     3841 gcgctgaacc ggaccggccg cgaggagatg ctgcgcagcg agctggtctc gcgtggggcg
     3901 cgcgtctcgg tcggggacgt cgtcgtcggg ctcaatgcgc gcatccatcc ggagcagacg
     3961 ctggaggacg tactcgatgt cctcctggcc catggccagc tcggccccgc gctggtcagc
     4021 gacgagggcc gcgtcagcgg catcctctcg ctggatcaac tccgccgctg gccgcgcgcc
     4081 ggctgggccg cgcataccgt cgccgaggcc atgatcccgc tggcgcgggt tgcctccatc
     4141 agcgccgacg cgtcggtacg cgagctgctc gaccgactgg tcgatgggca ggtcgaggtg
     4201 ctcgcggtcg tcaagcaggg cgaggtgctc ggcgccgtgg accggcgcct ggccgcccgg
     4261 caactcttcg agcgctcccg cgccgagaca cagcacaagg cgcgatagtg cgtcccgcca
     4321 cgaccgcgat tgggtataat caagcgcaca tgcatccagg ctgacgcgcg gcgggggtta
     4381 tccactccag gccgctcgtt gaatgagaga ggtccaccat aaccaccacc aacaccacga
     4441 tccccgcgcg cgagcgcgcc ctccttgtgg cagtcgattg gcagcgcaac ggctgggaca
     4501 tcggagcctc gctggaggag ctcgccaggc tcgccgagac ggccgacctc gaagtggcgg
     4561 acgccgtcac ccagcagctc gcacacccgc acgccggtca ctatctcggt tccggcaagc
     4621 tcgacgagat cgtcagccga tgccaggagg gtgacttcga cgtcgtgctg accaacgacg
     4681 agctggcccc cgcgcagctc cggtccctgg aggacgcgct gcagctcaag gtcatcgacc
     4741 gcaccgcact gatcctcgat atcttcgccg gccgcgcgcg cacgcacgag ggtcgcctcc
     4801 aggtcgagct ggcgcagctc gagtatcggc tgccgcgcct gacccgcatg tggacgcact
     4861 tgtcgcggca ggccgtgggc ggtgtcggcc tgcgtggccc cggtgagacg cagttggagg
     4921 tcgaccggcg cgaagcgcgc cggcgcatcg cgtggatcaa ggagcagttg gccgaggtcc
     4981 accagcaccg cgagctgtac cgcgagcggc gtcgccgcgg caagatcccg gtcgtcagcc
     5041 tggtcggtta caccaatgcc ggcaaatcga ccttgctcaa cgcgctcgcc ggcccgtcga
     5101 cgctggccga ggacaagctc ttcgcaacac tcgacccgac gacccgtcgc atcagcttgc
     5161 cctccgggcg tgaggtgctc ctgaccgaca ccgtcggctt cattcagaac ctgccgacgt
     5221 tcctggtcgc ggccttccgc gcgaccctgg aggagatcct ggaagcgtcg atcctggttc
     5281 acgtggtcga ctccacccac ccgatggccg agcagcaggg cgcgaccgtg aatgccgtgc
     5341 tcgaagaact gggcgccgac agccggccca cgatcacggc actcaacaag atcgacgccg
     5401 taagcgagat ggccaacggc tccggctccg ttctcccctt cgcgagcgcc gagcatctgg
     5461 tcggcatctc agcgcagacc ggcgaggggc tcgacggctt gctccggcag atcgaggtgc
     5521 tgctggaggc ggagcaacag atggtcgacg ttcgcctgct gattcccttc caccaggcac
     5581 gactcgtcga tctcttccac cgccgcggca aggtcgagcg tgaggatttc aacgaggagg
     5641 gaaccgttct cgacggccgc ctcccggcgc atctcctgcc ggccttcgcc gattaccggg
     5701 ctgggccggt tcaggacggt tctccaggag cggcagggcg ctagtacttt agacgcgaaa
     5761 ggccatcagc ggcgtctcgc ccgctcgata cgccatgctc gccccgctat aatcggtggc
     5821 acgtggtgtg gcgagagcgc gaaaggaacg ccgatggccg ggacgctgac ggacgtgctg
     5881 cagggtctac gccagggggg cggcttcgac atggtcgcgg tcgtcggcgc ggatggtctg
     5941 ccgatcgact cctgcaacgc gcccgacatg gaccccgatt ctctggccgc gctggccgcc
     6001 agcggactgc tcatgatgga cgcgatcggc caagagctgc ggcaggggaa ggcgacccag
     6061 gccatcttgg agttcgagcg cagcctcgtg gtgctcgaac cggtgcagca tgatctcgtg
     6121 ctgatggcgg tggcaaacgg cgagtcgaac ctgggacggg ttcgccttgt cctgcgccgc
     6181 gccctgccgg aggtcgccca ggcgatctcc gccttctgag gagcccacct cccgtgagtc
     6241 agccagcgcg accggggagc taccagtcgg ccggcgtcga tatcgagcgc gccgagcgcg
     6301 ccaaggcgcg catcgcggct cgcgtggccg cgctggccaa ccccaacgtc atcggcggta
     6361 tcggtcattt tggcggcttc ttccgcgccc ctcggagtga caagcccctg gtgctcgtct
     6421 caaccgcgga tagcgtcggc acgaaggtga tgctggccgc cgccaccggg aagcactacg
     6481 gtatcggcgt cgacctggtc aaccattgtg tcaatgacct gctggcgact ggagcgcgcc
     6541 cccttttctt cctggactat tacgcgacac ccgagcttga tcccgaggcg ctggccgaga
     6601 tcgtcg
//
LOCUS       DASUBI010000081         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1332915, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000081 DASUBI010000000
VERSION     DASUBI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332915"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1836
                     /locus_tag="VFU72_01510"
     CDS             508..1836
                     /locus_tag="VFU72_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422194.1"
                     /translation="MRVVQGKGGVLAGVLERPRAYALVLRSPEHNLLRNSLFIMLNTF
                     LGSGFGLVIWVAAARLYAPDQLGLGAAYISTIAFLAGLAELGLGTAIIRFGPGLREGR
                     NVFLNSALALVALAMLLATAVFALGTPLWSPRMGALTHSRFTLAVFIAAGVMTALDQL
                     FDKIYIAFESAHFLVLTNLLTNALRLAATIALSFFLGATGFVLALCVGVAAALVLSGW
                     RLAPRTVAGFALQPALSVAAVRSRLGYTIGNHLGALATLLPAFCYPLLIVNLIGAAAN
                     ATFYPIWMIANLLFVIPLSVATSAFARASNSEATAGDALAGAMKRLLLVLVVPAAGLV
                     AVSPLAFRVLGLRGTRAEFLLLAWLVLSVFPYAVNTVAITFFRVTLNTRAVIVVSGVA
                     SVACLAFSLAGALRLGLTGIALGWLLGQLAGAAVAALLVSVRRRAAPAAA"
     gene            complement(1764..2453)
                     /locus_tag="VFU72_01515"
     CDS             complement(1764..2453)
                     /locus_tag="VFU72_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5422195.1"
                     /translation="MNGHTLYVHLILLPYRQPRRTTAAAVAARCKESSKHPSRSRLAL
                     LLYPLLADRLLTARRLPLPLLALLDESAHLATTALLVPRRAPLGRWEALGAAAGSVLV
                     DLDHLPHYFRTSQPRPPGRPAPHSALSVATLLGLAWALALSERRRPRRLALGAAFGLT
                     CHLARDAATGGVPLGWPAGTGNVTISRPGYIVLLLRGALGVRPARAHAAAGAARRRTE
                     TSSAATAAPAS"
     gene            2477..3268
                     /locus_tag="VFU72_01520"
     CDS             2477..3268
                     /locus_tag="VFU72_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5422196.1"
                     /translation="MKLSPVARGARLELTPVRKLDEQQRKFCPLLRFGPQRTCAQGAG
                     RTVSPDYQQRFCLTAGFSQCPLYTGPRLAAPGAEEPAPSTLAGPCRRRYARPLVAGYA
                     ISAALALGAAAGTLWQLTNGAEHPASTPAISATAPAQPGALAASATPLPAATLAPPAV
                     TPVPASAAPRPSATAAVPLAAPAASSPAPEPTATPGSTPPPVAAGAPATAAPQATVYT
                     VQPGDTLHAIASRFHTTVDALAQRNQIADPDVIRPGTVLQIPPSG"
     gene            complement(3285..>3756)
                     /locus_tag="VFU72_01525"
     CDS             complement(3285..>3756)
                     /locus_tag="VFU72_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5422197.1"
                     /translation="ERLALMVNCDALARFGHTSLRVTKRPEILGYFRRLVAELDLPVP
                     VDSTGWLPGSSDEWPFLVEGVPAVSARGTQTPEERSRGRGLDHTQADTVDKIDDWRAR
                     EAAVALARILVAVADDEERPGPKLARAAVFEQLRRDGAEEELRVQGRWHPESIL"
BASE COUNT          465 a         1396 c         1339 g          556 t
ORIGIN      
        1 cgtcgggctc tacaaccgcc aggaactgcc gggctagcgc gagtcgcgcg gctcccggcg
       61 gctacgcatc cgttgaggtg gttagggcgg gctcagttct ccatctctcc acactcctcc
      121 ccgcgcgagg agcccggtcg ctcgcgctgt cgccggctgg gctccagtgc taaactcgcc
      181 agcaaaatcg agcatcttca ttcgaactgg tgcaacaagt gggcaggggt cctgctgggc
      241 tgcctcctcg tacggacctg gcggatcgga cacccgctcg tgccaccccg ccggttcgag
      301 gttggcccac acggtcgggg gatacggacc tcgcgcggcc tgtcgagggc gatgccgcga
      361 cgctcgaatg ccgtagacag ggcgcgcgcc gcacgagtca gcgcgcaaag aacatcaacg
      421 tcttcgaacg tcagcggttg aggcccgcgc cgaggtgatc acgacgttgg gggcagggtt
      481 aggctctggc agggggtacg acgacaggtg cgagtcgtgc agggcaaggg aggggtgctg
      541 gcgggggtgc tcgagcgccc cagggcgtac gcgctggtgc tgcgctcgcc ggagcacaac
      601 ctgctgcgca acagcctgtt catcatgctc aacacctttc tcggctcggg cttcgggctg
      661 gtgatctggg tcgcggcggc gcgcctctac gcccctgatc aactcgggct gggggcggcc
      721 tacatctcga ccatcgcgtt tctggccggg ctggccgagc tgggactggg cacggccatc
      781 atccgcttcg gcccggggtt gcgcgagggc cggaacgtgt tcctcaactc ggccctggcg
      841 ctcgtcgcgc tggccatgct gctggccacg gccgtcttcg cgctgggcac gccgctctgg
      901 tcgccgcgca tgggtgcgct gacccactcg cgcttcacgc tggccgtctt catcgccgcc
      961 ggggtgatga ccgcgctcga tcagctcttc gacaagatct acatcgcctt cgaatccgcc
     1021 cacttcctgg tgctgacgaa cctgctcacc aacgcgctgc ggctggcggc gacgatcgcg
     1081 ctcagcttct tcctgggcgc caccggcttc gtgctggcgc tctgcgtggg ggtggccgcc
     1141 gcgctggtgc tctcgggctg gcgtctggcg ccgcgcacgg tggcgggctt cgcgctccag
     1201 cccgccctca gcgtggccgc ggtgcgcagc aggctgggct acaccatcgg caaccacctc
     1261 ggcgcgctgg ccaccctgct gccggccttc tgctatccac tcctcatcgt caacctgatc
     1321 ggcgcggcgg ccaatgcgac cttctatcct atctggatga tcgccaatct gctcttcgtc
     1381 atcccgctct cggtcgccac ctcggccttc gcgcgggcct cgaacagcga ggcgacggcg
     1441 ggcgacgcac tggcgggcgc gatgaagcgg ctgctgctgg tgctggtcgt gccggcggcc
     1501 gggctggtcg ccgtctcgcc gctcgccttt cgcgtgctgg gcctgcgcgg cacgcgcgcc
     1561 gagtttctgc tgctggcctg gctggtcctc tcggtctttc cgtatgccgt caacacggtc
     1621 gcgatcacct tcttccgggt gacgttgaac acgcgtgcgg tgatcgtggt ctcgggcgtg
     1681 gcgagcgtcg cctgcctggc attcagcctg gcgggtgcgc tgcggctggg tctgaccggc
     1741 atcgcgctcg gctggctgct gggtcagctc gccggggcgg ccgtggccgc gctgctggtc
     1801 tccgtgcggc gacgagccgc gccggcggcg gcgtgagcgc gcgcagggcg cacgccgagc
     1861 gcgccgcgca ggaggaggac gatgtagccg gggcgcgaga tggtgacgtt gcccgtgccc
     1921 gccggccagc cgagcggtac gccgccggtg gcggcatcgc gcgccagatg gcaggtgagc
     1981 ccgaaggccg cgcccagggc gaggcggcgt ggtcgccggc gctccgacag cgccagcgcc
     2041 catgccagcc ccagcagcgt ggcgacggac agcgcggagt gcggcgccgg ccggccgggc
     2101 ggacgcggct ggctcgtgcg aaaatagtgg gggagatgat ccaggtcgac gagcacactg
     2161 ccggccgccg cgccgagcgc ttcccagcgc ccgagcgggg cgcgccgggg gacgagcagc
     2221 gcggtggtgg ccaggtgggc cgattcgtcg agcagcgcga gcagcggcag cggcagtcgc
     2281 cgggcggtca ggagccggtc ggccagcagc gggtagagca gcagcgcgag acgcgaccgc
     2341 gacggatgct tcgaggattc cttgcaccgc gccgcaaccg cggccgcggt cgtgcgcctc
     2401 ggctggcggt agggcaagag gataagatgt acgtacagcg tgtggccgtt catcttcacg
     2461 gcgaggtcgc atccctatga agctgtcccc ggtcgccagg ggggcgaggc tggaactgac
     2521 tcccgtacgc aagctggacg agcagcagcg gaagttctgc ccgctgctgc gcttcggtcc
     2581 gcagcgcacc tgcgcccagg gtgccgggcg caccgtctcg cccgactacc agcagcgctt
     2641 ctgcctcacc gccggtttca gccaatgccc gctctacacc ggcccgcggc tggcggcgcc
     2701 tggcgccgag gagcccgcgc ccagcacgct cgccggaccg tgccggcgcc ggtacgcgcg
     2761 cccgttggtc gccggctacg ccatctccgc cgccctggcg ctgggggcgg cggccggcac
     2821 gctctggcag ttgaccaacg gggccgagca tccggcctca acgccggcca tcagcgcgac
     2881 tgctccggcc cagccgggcg cgctcgccgc gtcggccacg ccgctgccgg cggccacgct
     2941 cgcgccgccg gccgtcaccc cggtcccggc cagcgccgcg ccgcgaccga gcgcgaccgc
     3001 ggccgtgccc ctggccgcgc ccgccgcctc ctcgccggcc ccggagccca cggcgacgcc
     3061 cgggtcaaca ccgccgcccg tggccgccgg cgccccggcc accgcggcgc ctcaggcaac
     3121 cgtctacacc gtccagccgg gcgacacgct ccacgccatc gccagccgct ttcacaccac
     3181 cgtcgacgct ctggcgcagc gtaaccagat cgcggacccc gacgtgatcc ggcccggcac
     3241 ggtgctccag atcccgccgt ccggctaatc gcgcgacggc gctgttacag gatgctctcg
     3301 gggtgccagc ggccctgcac ccgcagctcc tcctcggcgc cgtcgcgccg caactgctcg
     3361 aacacggccg cgcgggccag cttcgggccg ggccgctcct cgtcgtcggc taccgccacc
     3421 aggatgcgtg ccagcgcgac cgccgcctcg cgcgcccgcc agtcgtcgat cttgtccacc
     3481 gtgtccgcct gggtgtggtc cagcccgcgc ccgcgcgagc gctcctccgg cgtctgcgtg
     3541 ccgcgcgccg agaccgccgg caccccttcg accagaaacg gccattcgtc ggagctgccg
     3601 ggcagccagc cagtggagtc gacggggacc ggcaggtcca gctccgcgac caggcgccgg
     3661 aagtagccca ggatctcggg gcgcttggtc acccgcagcg aggtgtggcc gaagcgcgcc
     3721 agggcgtcgc agttgaccat caaggccagc cgctcc
//
LOCUS       DASUBI010000082         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_37644, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000082 DASUBI010000000
VERSION     DASUBI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37644"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFU72_01530"
     CDS             complement(<1..908)
                     /locus_tag="VFU72_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5422198.1"
                     /translation="MDLRYSPEDEAFRAEVRAWLAANLPAEKLETFEQQRAWHRKLYD
                     AGYVGMGWPVEYGGRDARPMEQAILGEEMARVNAPSSVNGLGIGLIGPTIIHHGTEAQ
                     KRRYLKNILSAEEIWCQLYSEPNSGSDLASLRTRAELVGDHFVINGQKIWTSSAHHAD
                     WGMRLARTDPDAPKHLGISCILIDMHHPGVNVRPLKQLTGGAEFNEVFFTNVVMPAEN
                     LVGDLHAGWRIAQTTLSYERGGNTLSRVSAQQAEFARLLEVARTLQRNGRPAIEDPVV
                     RQKLGQIYAEIEVLRYGSLRILSRLE"
     gene            complement(1092..2120)
                     /locus_tag="VFU72_01535"
     CDS             complement(1092..2120)
                     /locus_tag="VFU72_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5422199.1"
                     /translation="MRELNGTSQPAERQSDEELLRRIAGASAEALQELHHRYAPLIFN
                     LAAQSLDRPAAEEIVQDVFVALWRRADTFDPEKGSARGWILRIAHLRVLNELRRRSRR
                     PRAIPDPEGLHLGALPDAGPDPGEVVWQDYRRATLRDAVNLLPPAQRQALSLAYFDDL
                     THEQIAAYLELPLGTVKTRIRSGVQKLRVALMVALVLVAVVAGSIVAAVEARLHRQQS
                     RLDQEARALQAVTSSDVVPLRLSAAPGVPAATHAIYRSRPSGDLAVITFEHFPPAPSG
                     QVYQFWLRRNGTWLPLTTVTPQPDGSALVVVEGHGAGPSPDGLEVTLEPAGGRAAPSG
                     PVVVAWSP"
     gene            complement(2234..3196)
                     /locus_tag="VFU72_01540"
     CDS             complement(2234..3196)
                     /locus_tag="VFU72_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5422200.1"
                     /translation="MTTDQTTYQDSAAPDAPDGIDRRHFLKCMAWAGTGVLWTVSGGV
                     LSSCTFGGRAAAKQGDFTFAQISDSHIGFDKEPNTDVTATLHEAIAKVNALPERPAFV
                     LHTGDLSHLSKPDEFDTVEQSLKSDKTGQVFYFPGEHDVFSDNGQQYFDRFGTGTSGQ
                     GWRSFDYKGVHFVGLNNVMNLQTNGLGHLGPEQLDWLRKDVAGLSNSTPVVVFAHIPL
                     WMIYPSWGWGTEDAEQALSSLRRFGSVTVLNGHIHQVMQKVEGNITFHTARGTAFPQP
                     APGTAPAPGPLKVPADQLRKTLGIASVSYLEHKRSLAIVDSTLG"
     gene            complement(3193..3519)
                     /locus_tag="VFU72_01545"
     CDS             complement(3193..3519)
                     /locus_tag="VFU72_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEU5422201.1"
                     /translation="MTGMTGHVAMGTMTPGQQPVAAATPPLNQVFIDNFAFQPASLTV
                     PVGTEVTWTNHDDVPHTVTSRTKGVFSSQALDTDDHFSFRFDRAGTYAYYCAIHPIMN
                     GEVIVR"
     gene            complement(4084..>5239)
                     /locus_tag="VFU72_01550"
     CDS             complement(4084..>5239)
                     /locus_tag="VFU72_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422202.1"
                     /translation="TLQGAGAEERVVAHFRRLNQRRRGAVLADRLATQVVEAIAGSSI
                     NLGTGLLMLLAAGGLRSGSLTVGDFVLFVAYLGGIAGFTDGLGRFLAHYHQTGVAFSR
                     MHELLGAAPPARLVASSSLQLRGPLPELPAPAHVPDDRLTLLEARGLGYRYPETGRGI
                     TGVDLRLPRGSLTVVTGRVGAGKTTLLRALLGLLPLHAGEIRWNGHIVADPACSLVPP
                     RAAYTAQTPRLFSDTLRRNILLGLPGDAAALQAALHGAVLEDDVAALEAGLETAVGTR
                     GVKLSGGQVQRAAAARMLVRGAELLVIDDLSSALDVETEQALWERLCAGGDVTCLAVS
                     HRRAALHRADHIIVLKDGRVEAEGSLDLLLTTSPEMRALWHDADEPDGVG"
BASE COUNT          749 a         1772 c         1906 g          812 t
ORIGIN      
        1 tgctccagcc gcgagaggat gcggagcgag ccgtagcgca gcacctcgat ttcagcgtag
       61 atctgcccga gcttctggcg gacaaccggg tcttcgatgg ccgggcggcc attgcgctga
      121 agcgtgcggg cgacttccag caggcgcgcg aactcggcct gttgggcgct gacgcgactg
      181 agcgtgttgc cgccgcgttc gtaggagagg gtcgtctggg cgatgcgcca gccggcgtgc
      241 aggtcgccga ccagattctc ggccggcatg acgacgttgg tgaagaagac ctcattgaac
      301 tcggcgccgc cggtcaactg cttcagcggc cggacgttga cgccggggtg gtgcatgtcg
      361 atcaggatgc aggagatgcc gagatgcttg ggcgcgtcgg ggtcggtgcg cgccagcctc
      421 atgccccagt cggcgtggtg ggcactgctg gtccagatct tctgaccgtt gatcacgaag
      481 tggtcgccga ccagctcggc gcgcgtgcgc aggctggcca ggtcggagcc ggagttgggc
      541 tcggagtaga gctggcacca gatctcttcg gccgagagaa tgttcttgag gtagcggcgc
      601 ttctgcgcct cggtgccgtg gtggatgatg gtcgggccga tcaggccgat gcccagcccg
      661 ttgacgctgg agggcgcgtt gacccgcgcc atctcctcgc cgaggatggc ctgctccatc
      721 ggccgggcgt cgcgcccgcc gtactcgacc ggccaaccca tgccgacgta gccggcgtcg
      781 tagagcttgc ggtgccaggc gcgctgctgc tcgaacgtct ccagcttctc ggctgggaga
      841 ttggcggcca gccaggcgcg tacctcggcc cggaaggctt cgtcttcggg cgagtagcgg
      901 aggtccatcg gacgttcctc cgttctccac ggcggatggc cgtggtgggc gcccgagacc
      961 tgagcgatga ccaggtgacg ggcgcgaggc tgcgggtgag atccaggggc agtatagacc
     1021 ggaccatcaa cccgcacaac gcttcgcgcg ccgtccccgc gccgtcagtc ggcgcgagac
     1081 cccacctcgc ctcagggtga ccaggcgacg acgaccggac cgctcggcgc ggcgcggccg
     1141 ccggcaggct ccagcgtgac ctccagccca tcgggggatg ggccggcgcc gtggccctcc
     1201 accaccacca gcgcgctgcc gtccggctgt ggcgtgaccg tcgtcagcgg cagccaggtc
     1261 ccattgcggc gcagccagaa ctggtagacc tggccgctgg gggccggcgg gaaatgttcg
     1321 aaggtgatca cggcgaggtc gccgctgggg cggctgcgat agatggcgtg ggttgccgcc
     1381 ggtacgccgg gcgccgcgct cagccgcagc gggaccacgt cgctcgacgt gaccgcctgc
     1441 aacgcgcgcg cctcttgatc gagccgcgac tgctgccggt gcaggcgtgc ttcgacggcg
     1501 gccacgatgc tcccggccac cacggccacc agcaccaggg ccaccatcag cgccacgcgc
     1561 agcttctgta cgccgctgcg gatgcgcgtc ttgacggtgc ccagcggcag ctccaggtag
     1621 gcggcgatct gctcgtgggt caggtcgtcg aaataggcca ggctgagcgc ctgccgttgc
     1681 gcgggcggca gcaggttgac cgcgtcgcgc agcgtcgcgc ggcggtagtc ctgccagacc
     1741 acctcgccgg ggtccgggcc ggcgtcgggc agcgcgccga ggtgcagccc ctcggggtcg
     1801 gggatggccc gcggccggcg gctgcggcgg cgcagctcgt tgagcacccg caggtgggcg
     1861 atgcgcagga tccagccgcg cgccgagcct ttctcggggt cgaaggtgtc ggcgcggcgc
     1921 cagagcgcca cgaagacatc ctgcacgatc tcctcggccg cgggccggtc gagcgactgc
     1981 gccgcgagat tgaagatgag cggggcgtag cggtgatgca gctcctggag cgcctcggcc
     2041 gacgcgccgg cgatccggcg caggagctcc tcatcgctct gccgttcagc tggctggctt
     2101 gtcccgttga gctccctcat cgccacgtcc tcgatccgta ccggccgcaa gatcgttcct
     2161 tccataaaga aaggtcgccg cgcggtgttg agtggcggat ggcaggggtt cgaccctgcc
     2221 acccactctc gtcctaaccg agggttgaat cgacgatcgc cagcgagcgc ttgtgctcca
     2281 ggtagctgac gctggcgatg ccgagcgtct tgcgcagttg atccgccggc actttcaacg
     2341 gcccgggtgc cggggccgtg ccgggggccg gctgcgggaa ggcggtgccc ctggcggtat
     2401 ggaaggtgat gttgccctcg accttctgca tcacctggtg gatatgcccg ttcagcacgg
     2461 tgaccgagcc gaaccgccgt agagaggaca acgcctgctc ggcgtcttcc gttccccagc
     2521 cccaggacgg gtagatcatc cagagcggaa tgtgggcgaa gacgaccacc ggggtgctgt
     2581 tggacaggcc ggcgacatcc ttccgcaacc aatcaagctg ctccggcccc aggtgcccta
     2641 gcccgttcgt ctggaggttc atcacgttgt tgagcccgac gaagtggacg cccttgtagt
     2701 cgaagctgcg ccagccctgg ccgctcgtgc cggtgccgaa gcggtcgaag tactgctggc
     2761 cgttgtcgct gaagacgtcg tgctcgcccg ggaagtagaa gacctggccg gtcttgtcgc
     2821 tcttgaggct ctgctccacc gtgtcgaact cgtccggctt cgagagatgg ctgagatcgc
     2881 cggtgtgcag cacgaaggcg ggccgctccg ggagcgcatt gaccttggcg atcgcctcgt
     2941 gcagggtggc ggtgacgtcg gtgttcggct ccttgtcgaa gccgatgtgg ctgtcgctga
     3001 tctgcgcgaa ggtgaaatcg ccctgcttgg ccgcggcccg cccgccgaag gtgcaggagc
     3061 tgagcacacc gccgctcacg gtccagagca cgccggtgcc ggcccaggcc atgcacttga
     3121 ggaagtggcg ccggtcgatg ccgtcgggcg cgtccggcgc ggcggagtcc tgataggtcg
     3181 tctggtcggt cgtcatcgta cgatcacctc tccgttcatg atcgggtgga tggcacagta
     3241 gtaggcgtag gtcccggcgc ggtcgaagcg gaagctgaag tggtcgtcgg tgtcgagcgc
     3301 ctgggaggag aagacgcctt tcgtccggct ggtcaccgtg tggggcacgt catcgtggtt
     3361 ggtccaggtc acctcggtcc caaccggcac ggtcaggctg gccggttgaa aggcgaagtt
     3421 gtcgatgaac acctggttca acggtggcgt cgccgccgcc accggctgct ggccgggggt
     3481 catggttccc atggccacgt ggcccgtcat gccggtcatg gacggcgcca ccgccggcgc
     3541 gccgggcgcg ttcgccgccc cggctccgcc acaggcagcc aagagggcag gtagcgcagt
     3601 cgcgctcaca gccagcgtgc ccaggcgcgc tagaaagcgg cgccgcgagc agtgatcgat
     3661 cgttagtccc gtcgctccgg cgctcgtcga accagacgag cctccggtcg tggttggtgc
     3721 acacaagtcc tgcattcctc tcgatagtgt cggcgccgaa gtcgacgtct cgctccggcg
     3781 cgggggccag gcgatccgaa ggacgcggcc ggaccgcctg gcctgacgtc gtgcgtcctc
     3841 gatccggctc tcaatacacc ggcctggcgg gtttggattt ggtcaaagac ggaattggag
     3901 cttcgttcgc tgcggggcgc tggatgagaa gacgtgcgtc tcccggtagg ggcgaccccc
     3961 cgtggtcgcc ctcgcccccc ggcgcatcgg caacgaggat atgggggatg ttgggtggcg
     4021 gacgacgagg gccaggggtg ggcacggagg cctgccccca caacgacggc gccgtggcag
     4081 acgctatccc accccgtccg gctcgtcggc gtcgtgccac agggcgcgca tctctggact
     4141 ggtggtcagg aggaggtcga ggctgccctc ggcctcgacc cggccgtcct tcagcacgat
     4201 gatgtggtcg gcccggtgca gcgccgcgcg ccggtgtgag acggccaggc aggtgacgtc
     4261 acccccggcg cagaggcgct cccagagcgc ctgctcggtc tcaacatcga gcgcgctgga
     4321 gaggtcgtcg atcaccagca gctcggcgcc gcgcaccagc atgcgggccg ccgccgcgcg
     4381 ctgcacctgg ccgcccgaga gcttcacccc gcgcgtgccg accgccgtct ccagccccgc
     4441 ctccagcgcc gccacgtcgt cctccagcac ggcaccgtgc agcgccgctt ggagcgccgc
     4501 cgcgtcgccg ggcaggccga gcaagatgtt ccgccgcagc gtgtcgctga agaggcgcgg
     4561 cgtctgggcg gtgtaggccg cgcgcggcgg caccagggag caggcgggat cggccacgat
     4621 gtgcccgttc cagcggatct cacccgcatg gagcggcagc aggccgagca gcgcccgcag
     4681 cagcgtggtc ttgcccgcgc cgacacgccc ggtcaccacc gtcagcgagc cgcgcggcag
     4741 gcgcaggtcg acaccggtga tgccgcgccc cgtctcgggg tagcgatagc ccagtccgcg
     4801 cgcctccagc agcgttagcc ggtcgtccgg gacgtgagcg ggcgccggca gctccggcag
     4861 cgggccgcgc agttggagcg agctgcttgc gaccagccgg gccggcggcg cggcgcccag
     4921 cagctcgtgc atgcggctga aagccacgcc ggtctggtgg tagtgcgcca ggaagcggcc
     4981 gagcccatcg gtgaacccgg cgatgccgcc caggtaggcg acgaacagga cgaagtcgcc
     5041 gaccgtcagg ctgccggagc gcagaccgcc ggcggccagc agcatgagca gcccggtgcc
     5101 taggttgatc gagctgcccg cgatggcctc caccacctgg gtcgccagcc ggtcggccag
     5161 caccgcccct cgacgccgct gattgagccg gcgaaagtgg gccacgacgc gctcctcggc
     5221 gccggccccc tgcagcgtg
//
LOCUS       DASUBI010000083         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_285482, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000083 DASUBI010000000
VERSION     DASUBI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285482"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFU72_01555"
     CDS             complement(<1..916)
                     /locus_tag="VFU72_01555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422203.1"
                     /translation="MALGGARYGLAIPQIFVAEPVDYELIHRAVRQAEELGYASLWTQ
                     EQIIGSAATLEPVSLLSYVAALTTHVRLGASVLVLPLRNPVQLAKSLATLDQLSSGRL
                     TVGLGLGGHLDRYAAFGLSPEHRVRRFNENLRVMRALWAEPSASVAGVFQQLRDLPME
                     PKPLQRPAPPIWFGGRQPAALRRAARQAEGWMGAGSSAISSFKEHVVLLREFLQGAGR
                     DAAQFTIAKRLYIAVDDDRARAEQRLRQWFRGYYGSDELALRVSVFGDAGECGARIAE
                     VVAAGAGEVLLNPVFDQLDQAERLAEIVG"
     gene            complement(1107..1182)
                     /locus_tag="VFU72_01560"
     tRNA            complement(1107..1182)
                     /locus_tag="VFU72_01560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1147..1149),aa:His,seq:gtg)
     gene            complement(1591..1677)
                     /locus_tag="VFU72_01565"
     tRNA            complement(1591..1677)
                     /locus_tag="VFU72_01565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1641..1643),aa:Leu,seq:cag)
     gene            complement(1679..1754)
                     /locus_tag="VFU72_01570"
     tRNA            complement(1679..1754)
                     /locus_tag="VFU72_01570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1719..1721),aa:Thr,seq:cgt)
     gene            1902..2840
                     /gene="mdh"
                     /locus_tag="VFU72_01575"
     CDS             1902..2840
                     /gene="mdh"
                     /locus_tag="VFU72_01575"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5422204.1"
                     /translation="MRGKVTVVGAGAVGATTAQYLADRNIADIVLVDIVENLPQGKAL
                     DLAEAGPVIGYDVAVRGSNSYDATAGSDVVVITSGSPRKPGMSRDDLLRVNMNIVGSV
                     AKQVAAQSPNAVIIVVTNPLDAMCHATLNATGFPAQRVIGQAGVLDSARFRAFIAMEL
                     DVSPRDVVAPVLGGHGDTMVPLVSYSSVSGIPLEQLLPRQKIDELVQRTRDGGGEIVK
                     LLGTSAYYAPAASVAEMVEAVLLDENRVIPCSVHLSGQYGIKDLYVGVPVKLGAGGIK
                     QIYELKLSDQERQALQHSADAVRAVVEDMKRLAQES"
     gene            2850..4133
                     /gene="glp"
                     /locus_tag="VFU72_01580"
     CDS             2850..4133
                     /gene="glp"
                     /locus_tag="VFU72_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5422205.1"
                     /translation="MTRGAAASAREWERPEALLGVDEARERILAAVAPLPPERLPLLD
                     ALGLVLSEDVVATTDLPPFRNSAMDGYAVQAVDTTGAKPDRPVVLRVVGEVPAGVASA
                     LSVGPGESARIMTGALVPDGADAVVRFEDTDELAQPGPRNKVLVHRAVGRCANVRQAG
                     EDVARGSLVLPVGAEVQPATLGLLAALGCTSVPVHRPPRVAILSTGDELVPAGEPLAP
                     GQIRDSNTIMLAALVKQLGARPVSLGAVRDDSDEIRARLSQARDADLLLTSGGVSVGD
                     YDLVKRVLRQEGRIDLWQVRIKPGKPMAFGRIGATPLIGLPGNPTAALVAFLQFARPA
                     IQRMLGRLHWTPPLARARLSCTLENHARRRHFVRGVVVRCGDTLLARPAETQGSASLS
                     SVVRANCLIIVPEEWDHAPAGTDVDVELLDIFAPR"
     gene            4197..>5031
                     /locus_tag="VFU72_01585"
     CDS             4197..>5031
                     /locus_tag="VFU72_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422206.1"
                     /translation="MAVRDLLSFRDYFNIIRQLSFRELDGEARDLPAILVVAESRELA
                     VLAGEALFGHAGAAFVEAHAFDAQLDPLRYDVILTIDRLPTKLRHDWSRLFNRAHEEL
                     RLIEVLPRVVGDAEAERAARTRVVDRAEEKRLLALGRHIPLLRAACAHRVVWDTSMAD
                     GQFALVSNIPEVVPIIGNLIAAGADFLVLTKNQLLMLYKLAAIFGRDLGNRRRIYSEM
                     LPVVGAGLVWRTVARELVTLMPFGLGTVPKVAIAFAGTWAVGQAAVVYYESGEKLSRE
                     QM"
BASE COUNT          774 a         1698 c         1761 g          798 t
ORIGIN      
        1 aaccaacgat ctccgctagc cgctctgcct ggtcgagctg gtcgaagacc gggttgagca
       61 gaacctcgcc cgccccagca gcgacgacct cggcgattcg agcgccgcac tcaccggcat
      121 caccgaagac gctgacgcgc agggccaact cgtcggaacc gtagtagcca cggaaccatt
      181 ggcgcaagcg ctgttcggcg cgggcgcgat cgtcgtcgac cgcgatatag aggcgcttgg
      241 cgatggtgaa ttgagccgcg tcgcgcccgg ccccctggag gaactcgcgc agcagcacca
      301 catgctcttt gaaggagctg atggcgctgg agccggcgcc catccagccc tcggcctggc
      361 gcgcggcccg gcgcagggcg gcaggctggc ggccgccgaa ccagatcggc ggtgccgggc
      421 gctgcaacgg cttgggttcc atgggaagat cgcgcagctg ctggaacacc ccggcgacgc
      481 tcgcggatgg ctcggcccag agcgcgcgca tgacgcgcaa gttctcgttg aagcgacgca
      541 cgcggtgctc gggcgagagt ccaaaggcgg cgtagcggtc aaggtggccg ccgagcccca
      601 gcccgacggt gagccggcca cttgagagct ggtcgagcgt ggccaggctc ttggcgagct
      661 gcaccgggtt gcgcagcggc agcaccagca ccgaggcgcc gagccggacg tgcgtagtca
      721 gggccgccac gtagctcaag aggctcaccg gctccagcgt ggcggcggac ccgatgatct
      781 gttcctgggt ccagaggctg gcgtagccca gctcttcggc ctgccgcacc gcgcggtgga
      841 tcagttcgta gtctaccggc tcagccacga agatctgcgg gatcgccagg ccgtagcgag
      901 cgccgcccaa tgccatcttg cacccctctc agcgtcgcgt tcgtccggcg cgatagcgcc
      961 tgtcgcgacg gtacagctag tgtacagttc gaacgggcag tccgagacgg tggtgtgagg
     1021 caagcatgta cgggctcggg cggctggctg ccggccaaga atccgcagcg ggacaacaaa
     1081 aaagacctcc cgaaggaggt cagttttggg gtgtcccgcg gggcttgaac ccgcaacccc
     1141 tggttccaca gaccagtgct ctgccgattg agctagggac accgtgccga atgatgccgg
     1201 gcgagtaagc cgagttctgt tttatacggt catctgtctc ggcggcaagc cgccggccat
     1261 gccgtcgcca gcatggctgc tctcgtcttc gggcctgggc gagtcacctc gtagcggccc
     1321 ggccggagat tgctgcgggg aggattgccc ttttcactcc gcgccaagcg cggcctggtc
     1381 tctgttgctc tcgcgcggtt gcccgcgcgc cgatctggac tgtcagcgtc accgacgaac
     1441 gctgccccgg ctttcaccgg gcaccccgac tcgcgcagct cggactttcc tctggacgag
     1501 cgacgttgcc gtcgtcgcca gcgaccgtgc tgcccagcat cactcagttg ttaaagacgg
     1561 cccgcctggt tgcccaggcg gaccggccgg tggtgccgaa ggggagattt gaactcccac
     1621 gggcaattgc ccactacgcc ctgaacgtag cgcgtctgcc agttccgcca cttcggcctg
     1681 gagccgacca tcggactcga accgatgacc tggtgtttac gaaacacctg ctctaccaac
     1741 tgagctaggt cggcggatcg gttctcccga cgcgctcgaa agtatagcac aggcggcctt
     1801 cgctcatcaa gcccgccgcg aagtatcggg gccctgtgct atcatgctgc caggcgccgg
     1861 atgccaatcg gcgccgcacg acggcaagca tgggaggcgg gatgcgcgga aaagtcacgg
     1921 tggtcggcgc gggcgcggtg ggcgccacca cggcgcagta tcttgcggat cgcaatatcg
     1981 ccgacatcgt cctggtcgac atcgtcgaga atctgccgca gggcaaggcg ctcgatctgg
     2041 ccgaggccgg gccggtcatc ggctacgatg tcgccgtgcg cggcagcaac tcctacgatg
     2101 ccaccgccgg ctccgacgtg gtcgtcatca cctccggctc gccgcgcaag cccggcatga
     2161 gccgggatga cctcctgcgg gtcaacatga acatcgtcgg ctcggtcgcc aagcaggtgg
     2221 cggcgcagtc gcccaacgcg gtgatcatcg tggtgaccaa cccgctcgac gcgatgtgcc
     2281 acgccaccct caacgcgacc ggtttcccgg cgcagcgcgt gatcggccag gccggtgtgc
     2341 tcgactcggc ccgcttccgc gccttcatcg ccatggagct cgacgtctcg ccgcgcgacg
     2401 tggtcgctcc ggtgctcggg ggccacggcg acacgatggt gccgctggtc agctactctt
     2461 cggtcagcgg catcccgctg gagcagttgc tgccgcgcca gaagatcgac gagctggtgc
     2521 agcgcacgcg cgacgggggc ggcgagatcg tcaagctgct cggcaccagc gcctactacg
     2581 cgccggccgc gtcggtggcg gagatggtcg aggccgtgct gctggacgag aaccgcgtga
     2641 tcccgtgcag cgtgcacctc agcggccagt acggcatcaa ggacctctac gtcggggtgc
     2701 cggtcaaact cggcgcgggc ggaatcaagc agatctacga gctgaagctc tccgaccagg
     2761 agcggcaggc gctgcagcac tcggcggacg cggtgcgcgc ggtggtcgag gacatgaagc
     2821 gactcgcgca ggagtcgtag gagcatggca tgacccgcgg cgcggcagcc agcgctcgcg
     2881 agtgggagcg ccccgaggcg ctgctgggcg tcgacgaggc ccgcgagcgc atcctggccg
     2941 cggttgctcc acttcctccc gagcggctgc cgctgctcga cgccctgggc ctggtgctgt
     3001 ccgaagacgt cgtggccacc acagacctcc cacccttccg caactcggcg atggacggct
     3061 atgcggtgca ggccgtagat acgacgggcg cgaagccgga ccgcccggtc gtcctgcgcg
     3121 tggtcggcga ggttccggcg ggcgtggcgt cggcactctc ggtcggaccc ggcgagagcg
     3181 cgcgcatcat gaccggcgcg ctcgtgcccg acggcgccga cgcggtggtg cgctttgaag
     3241 acaccgacga gctggcccaa cctgggccgc gcaacaaagt cttggtccac cgcgccgtgg
     3301 gacgctgcgc caacgtgcgc caggcaggcg aggatgtcgc gcggggcagc ctggtgctgc
     3361 ccgtgggcgc cgaagtgcaa cccgccacgc tggggctact ggctgctcta ggctgtacca
     3421 gcgtgcccgt gcatcgcccg ccgcgcgtcg cgatcctctc cacgggtgac gagctggtgc
     3481 cggccggaga gccgttggcg ccggggcaga ttcgcgacag caacacgatc atgctggccg
     3541 cgctggtcaa acagctcggc gcgcggcccg tttcattggg cgcggtccgc gatgactcgg
     3601 atgagatccg agcgcgactc tcccaggcgc gcgacgccga tctgctgctg acctccggcg
     3661 gcgtctccgt gggggactac gatctggtca aacgcgtgct gcgtcaggag ggtcgcatcg
     3721 acctgtggca ggtacgcatc aagcccggca agccgatggc gttcgggcgc atcggggcca
     3781 cgccgctgat cgggctgccg gggaatccga cggcggcgct ggtcgccttc ctgcaattcg
     3841 cccggccggc catccagcgc atgctggggc ggctgcattg gacgccgccg ctggcgcggg
     3901 cgcgtttgag ctgtaccctg gagaaccacg cccgccggcg gcatttcgtg cgcggggtgg
     3961 tggtgcgctg cggcgacacg ttgctggcgc gcccggccga gacgcaaggt tcggcctcgc
     4021 tcagctccgt cgtccgagcc aactgcctga tcatcgtccc ggaggagtgg gatcacgctc
     4081 cggccggtac cgacgtcgac gtcgaattgc tggacatctt cgccccgcgt tgagccgggc
     4141 acgccgcgtg cgcctgcgga cccggtgaga taccgccggg gggaggaacg caactcatgg
     4201 ccgtccgcga cctgctgtcg tttcgtgact acttcaacat catccgccaa ttgagcttcc
     4261 gtgagctgga cggggaggca cgggatctcc cggccatcct ggtagtggcc gagagccgcg
     4321 agctggccgt gctggcgggt gaggcgctct tcggacacgc gggcgccgcc ttcgtggagg
     4381 cacacgcgtt tgacgctcag ctcgatccgc tgcgctacga cgtgatcctg acgatcgatc
     4441 ggttgcccac aaagctacgt catgactgga gccggctctt caaccgtgcg cacgaagagc
     4501 ttcggctgat cgaggtgctg ccgcgcgtgg tcggcgatgc cgaggccgag cgtgccgcgc
     4561 gcacccgggt ggtcgaccgc gccgaggaga agcgcctgct ggcgctcggg cggcacatcc
     4621 cgctgctgcg ggcggcctgc gcgcaccggg tcgtctggga cacgtcgatg gccgatggac
     4681 agttcgcgct ggtctcgaac atccccgaag tggtgccgat catcggcaac ctcatcgcgg
     4741 ccggcgccga tttcctggtg ttgaccaaga accagctctt gatgctctac aagctggcgg
     4801 cgatcttcgg ccgcgatctg ggcaaccggc ggcgcatcta ttccgagatg ctgccggtgg
     4861 tcggcgccgg gctcgtctgg cgcaccgtcg cgcgcgagct ggtgacgctg atgccgttcg
     4921 ggctcgggac cgtccccaag gtggcgatcg ccttcgccgg cacctgggcg gtcgggcagg
     4981 cagccgtcgt ctactacgag agcggcgaga agctgagccg cgaacagatg c
//
LOCUS       DASUBI010000084         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_883064, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000084 DASUBI010000000
VERSION     DASUBI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883064"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="hutU"
                     /locus_tag="VFU72_01590"
     CDS             <1..1201
                     /gene="hutU"
                     /locus_tag="VFU72_01590"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5422207.1"
                     /translation="RQHVGGTLSGRLVLTAGLGGMGGAQPLAVTMNGGAALIVEADPE
                     RIARRVQLGYCDTATEDLDEALRLVEGARQRGEPLSVALLGNAAEVFPELVKRGFKPD
                     IVTDQTSAHDPLGGYIPAEISFEDAQLLREQNPELYTERSLTSMATHVRAMLAFQQGG
                     AVVFDYGNNLRGQALVAGEKNAFDYPGFVPAFIRPLFCRGKGPFRWAALSGDAEDIYR
                     TDAAIARLFPEDEALQRWLRLARERVPFQGLPARICWLGYGERHRAGLEFNRMVRAGE
                     LSAPIVIGRDHLDAGSVASPYRETEAMRDGSDAIADWPILNALVNTAAGASWISVHHG
                     GGVGIGYSIHAGMVVVADGSERAERKLGRVLLTDPGTGVLRHADAGYEEALEAAEEFG
                     LRLPMRE"
     gene            complement(1376..3160)
                     /locus_tag="VFU72_01595"
     CDS             complement(1376..3160)
                     /locus_tag="VFU72_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5422208.1"
                     /translation="MRLRVNLASNWLLDVEMKEYRPAEEVDFCIVGTGAGGGVLAQRL
                     ARDGYSVVALDAGPWHDSEHDMVSDEAGSAPLYWNDIRVSGGGEPLEFGANNSGRGVG
                     GSTIHFAAFMPRLHPSDFRVRTLDGVAADWPLAYEDLASYYEQMEREYPVTGPALYPW
                     GAPHGYPYAPLEAGTAGQMLIKGCVALGIPVVAGGPVGIPAGRVGKRPHCIMRGFCLL
                     GCKVGAKSSTLVSFIPDAIAHGAEIRTGCMAFDIPLDDAGHARGVRYYRTLENGQVIE
                     EEQRARAVVVAGYAIETPRLLLESSSARFPDGLANSSGLVGKFLMAQAAPVVWGRFEE
                     MIRQYKAPPACALTEEFYETDPANDFVRGYALQSVSPLPIAMAHVLTESGYWGEALLQ
                     RMQLYNHYACLGVLGEILPDERNFVALDPEVKDQYGLPAPRAEFNLFPNDLKLMEAGT
                     ARAEAVLRAAGAVETGSVRRYAHLVGTCRMGFSPRDSVVDQYCRAWDVPNLFVCDGSV
                     LPTQGSSNPGLTISALAARTADWIRDHAPNGELAQRPRAQAPSQRAAPGRTRKLWSML
                     GWIAHSEQIPAGSVADWEQGRGRDDRRR"
     gene            complement(3157..3786)
                     /locus_tag="VFU72_01600"
     CDS             complement(3157..3786)
                     /locus_tag="VFU72_01600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU5422209.1"
                     /translation="MIKDLYPGWQALGQRGHWDEATRKVVLDRVEHVPPYRFFAPQQR
                     RTLEALAERVLPQAHRPPERRVPIGPWIDQLCAGRFEHGTRYADMPPQRDAWRLGLAG
                     LDQTSQAHYGAGFADLDAPRQDAVLRMIAEGNPLGDAWRELPARRWWQSLALRQISGV
                     YYAHPYAWDEIGWGGPAYPRGYYALNHGAPEPWEPRQRSGYSEEELRSP"
     gene            complement(3783..4730)
                     /locus_tag="VFU72_01605"
     CDS             complement(3783..4730)
                     /locus_tag="VFU72_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422210.1"
                     /translation="MGRGGWPGVRRELAVARVEIRQGRFQRTMALLSAFSAIVSGFEA
                     YTQHERGAFSHWLMWTPVWLTPPATLAGIAAVFSRRVARLVLPVVALASLIDGLLGFI
                     YHIRGVRGLPGGFRLGQYNLVIGPPLFAPVLTGMVGVLGLLASALRREQLGWFESRSP
                     SLGQLQRLGRFDTRQASLPALLAHGEFQRGMALLAAVFAALAGGEAYLEHLRGSFNRR
                     VMWTPVWVTPPMIAAGIAAALDRRAARLVLPFVSLAALADGALGFVLHVQGLQRMPGG
                     VRNLRFNLVLGPPLFAPLLFSAVGLLGLIATLLRRRDGA"
     gene            complement(4749..4958)
                     /locus_tag="VFU72_01610"
     CDS             complement(4749..4958)
                     /locus_tag="VFU72_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422211.1"
                     /translation="MRGVHELLTAILEGAVALVALLIAGGLLYFVAGHLIGGARREVR
                     KRRPAPSPGAVPDERHQNVTAVSVR"
     gene            complement(4955..>5438)
                     /locus_tag="VFU72_01615"
     CDS             complement(4955..>5438)
                     /locus_tag="VFU72_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5422212.1"
                     /translation="AERFAELGVDWFEEPVYHHDLDGLRLLRDRAPAPLRIAAGEYGY
                     DLWHFRRMLEAGAVDVLQADVTRCGGVTAFLRTGALCEAHFLPLSAHCAPFLHASPGC
                     SLPSMLNIEYFYDHVRIAHLLFDGVLEPVDGALRPDRGRPGFGLELKRQDAERWRVAL
                     "
BASE COUNT          835 a         1940 c         1956 g          707 t
ORIGIN      
        1 ccgccagcac gtcggcggca cgctctcggg ccggctggtg ctgaccgccg ggctcggggg
       61 catgggcggc gcccagccgc tggcggtcac catgaacggc ggcgcggcgc tgatcgtcga
      121 ggccgacccc gagcgcatcg cgcgccgcgt gcagctcggc tactgcgaca ccgccaccga
      181 ggacctggac gaggcgctgc ggctggtcga gggcgcgcgc cagcggggcg agccgctctc
      241 ggtcgcgctg ctgggcaacg ccgccgaggt cttccccgag ctggtcaagc gcggcttcaa
      301 gccggatatc gtcaccgacc agacctcggc ccacgacccg ctgggcggct acatcccggc
      361 cgagatctcg ttcgaggacg cgcaattgct ccgcgagcag aaccccgagc tctacaccga
      421 gcgcagcctg accagcatgg ccacgcacgt gcgcgccatg ctcgccttcc agcagggcgg
      481 cgcggtcgtc ttcgactacg gcaacaacct gcgcggccag gcgttggtag ccggggagaa
      541 gaacgccttc gactaccccg gcttcgtgcc ggcctttatc cggcccctct tctgccgcgg
      601 caaagggccc ttccgctggg ccgcgctctc cggcgacgcg gaggacatct atcgcaccga
      661 cgcggccatc gcgcgcctct tccccgagga cgaggcgctg cagcgctggc tgcggctggc
      721 gcgcgagcgc gtgcccttcc aggggctgcc ggcgcgcatc tgctggctcg gctacggcga
      781 gcggcaccgc gccgggttgg agttcaaccg catggtgcgc gcgggcgagc tatcggcccc
      841 gatcgtgatc gggcgcgacc atctggatgc cggctcggtc gcctcgccct accgcgagac
      901 cgaggccatg cgcgacggct ccgacgccat cgccgactgg cccatcctga atgccctggt
      961 caacacggcg gccggcgcga gctggatcag cgtgcaccac ggcggcgggg tcggcatcgg
     1021 ctacagcatc cacgcgggca tggtggtggt ggccgacggc agcgagcgcg ccgagcgcaa
     1081 gctgggccgc gtgctcttga ccgaccccgg caccggggtc ctgcgccacg ccgacgccgg
     1141 ctacgaggag gcgctggagg ccgccgagga gttcggcctg cgcctgccga tgcgggagta
     1201 gaaggttttc agcctaccga catcgaatgg atgctcgtga tgacggatcc ttcgacttcg
     1261 ctcaggatga caatgtgaga tttgtggcct ttcggacgac gctcatcctc gtcgtccgcc
     1321 ccctcctaac cctgtcatcc tgagcgcagt cgaaggatcc gtcctcgccc ggcactcaac
     1381 gtcgtcggtc atcgcgcccg cgcccctgct cccaatccgc caccgacccc gcagggatct
     1441 gctccgagtg ggcgatccag ccgagcatcg accagagctt gcgcgtgcgg ccgggggcgg
     1501 cgcgctggga cggcgcctgg gcgcgcgggc gctgggcaag ctcaccattg ggagcgtggt
     1561 cgcggatcca atcggcggtg cgcgccgcca gcgccgagat cgtcaggccg gggttggacg
     1621 agccctgcgt cggcaagacg ctgccgtcgc agacgaagag gttaggcacg tcccaggcgc
     1681 ggcagtactg atcgaccacc gagtcgcgcg ggctgaagcc catgcggcag gtgccgacca
     1741 ggtgcgcgta gcggcgcacg gagccggtct cgaccgcgcc cgccgcgcgc agcaccgcct
     1801 cggcgcgcgc cgtccccgcc tccattagct tgaggtcgtt ggggaagagg ttgaactcgg
     1861 cgcgcggcgc gggcaggccg tactggtcct tgacctcggg atcgagcgcc acgaagttgc
     1921 gctcgtccgg caaaatctcg cccagcacgc cgaggcaggc gtagtggttg tagagctgca
     1981 tgcgctgcag cagggcctca ccccagtagc cgctctcggt cagcacgtgc gccatcgcga
     2041 tcggcagcgg tgagaccgac tgcagggcgt aaccgcgcac gaagtcattc gcgggatcgg
     2101 tctcatagaa ctcctcggtc agggcgcagg ccggcggggc cttgtactgc cggatcatct
     2161 cctcgaagcg gccccagacg accggcgcgg cctgggccat caggaacttc cccaccagcc
     2221 cactggagtt ggccagcccg tcggggaagc gcgccgagct ggattccaag agcagccggg
     2281 gcgtctcgat ggcgtagcct gccaccacca ccgcgcgggc gcgctgctcc tcctcgatca
     2341 cctgcccatt ctccagcgtg cggtaatagc gcacgccacg agcgtggccg gcgtcgtcca
     2401 gcgggatatc gaaggccatg cagccggtcc gaatctcggc gccgtgcgcg atggcgtcgg
     2461 ggatgaagct gaccagggtg ctgctcttgg cgccgacctt gcagccgagc aggcagaagc
     2521 cgcgcatgat gcagtgcggg cgcttgccca cccgtcccgc cgggatgccg accggcccgc
     2581 cggccaccac cgggatgccc agcgcgacgc agcccttgat cagcatctgc cctgccgtgc
     2641 ccgcctccag cggcgcgtag gggtagccgt gtggcgcgcc ccaggggtag agcgccggac
     2701 cggtgaccgg gtactcgcgc tccatctgct cgtagtacga cgccaggtct tcgtaggcga
     2761 gcggccagtc ggccgccacg ccatccagcg tgcgcacgcg gaagtcggag gggtgcaggc
     2821 gcggcatgaa ggcggcgaag tgaatcgtac tgccgccgac cccgcgaccg gagttgttgg
     2881 cgccgaactc cagcggttcg ccgccgccgc tgacgcggat gtcgttccag tagagcggcg
     2941 ccgagccggc ctcgtccgag accatgtcgt gctcgctgtc gtgccagggg ccggcatcca
     3001 gcgccacgac ggaatagccg tcgcgcgcca gccgctgggc cagcacgccg ccgccggcgc
     3061 cggtgccgac gatgcagaag tcgacctcct cggccgggcg gtactccttc atctcgacgt
     3121 cgagcagcca gttggaagcc agattgacgc gcagtctcac ggtgagcgca gctcctcctc
     3181 gctgtagccg ctccgctggc gcggctccca gggctcgggc gcgccgtggt tgagggcgta
     3241 gtagccgcgg gggtaggccg ggccgcccca gccgatctcg tcccaggcgt agggatgcgc
     3301 gtagtagacg ccgctgatct ggcgcagcgc caggctctgc caccagcggc gagccggcag
     3361 ctcgcgccag gcatcgccca gcgggttgcc ctccgcgatc atgcgcagca ccgcgtcctg
     3421 gcggggcgcg tccaggtcgg cgaagccggc gccgtagtga gcctgggaag tctgatccag
     3481 cccggccagg ccgagccgcc aggcgtcgcg ctgcggcggc atgtccgcgt agcgggtgcc
     3541 gtgctcgaag cgcccggcgc agagctggtc gatccagggg ccgatcggaa cgcgccgctc
     3601 gggcggccgg tgggcctgcg gcagcacgcg ctcggccagc gcctccagcg tacgccgctg
     3661 ctggggagca aagaagcggt agggcgggac gtgctcgacg cggtcgagca ccaccttgcg
     3721 cgtggcctcg tcccagtggc cgcgctggcc gagagcctgc cagccggggt agagatcctt
     3781 gatcatgcgc catccctccg gcgcagcagc gtcgcgatca gccccagcag cccgacggcg
     3841 ctgaagagga gcggcgcgaa gagcggcggg ccaagcacca ggttgaagcg caggttgcgc
     3901 acgccgcccg gcatgcgctg caatccctgc acgtgcagca caaagccgag ggcgccgtcg
     3961 gccagcgcgg ccagcgagac gaacggcaag acgagccgcg cggcgcggcg atcgagggcg
     4021 gcggcgatgc cagcggcgat catcggcggc gtgacccaga ccggtgtcca catgacgcgg
     4081 cggttgaagc tgccgcgcag gtgctccaga taggcctcgc cgccggccag cgcggcgaaa
     4141 acggcagcca ggagcgccat gccgcgctgg aactcgccgt gggccaatag agcgggcagg
     4201 ctggcctggc gcgtgtcgaa acggcccagc cgctgcagtt gcccaagcga gggcgagcga
     4261 ctctcgaacc agccgagctg ctcgcgacgc agggcgctgg ccagcagccc aagcacgccg
     4321 accatgccgg tcagaaccgg cgcgaagagc ggcggtccga tcaccaggtt gtactgtccc
     4381 aggcggaagc cgccggggag gccgcgcacc ccacggatgt gatagatgaa gccgagcagg
     4441 ccgtcgatca gcgaggccag cgccaccacc ggcagcacca ggcgggcgac gcgccggctg
     4501 aagacggcgg cgatcccggc caatgtggcc ggcggcgtca gccagaccgg cgtccacatc
     4561 agccagtggc tgaaggcgcc gcgctcgtgc tgggtgtagg cctcgaagcc gctgacgatg
     4621 gcggagaagg cgctgagcag cgccatcgtg cgctggaagc gcccctgccg gatctcgacg
     4681 cgcgccacgg ccagctcacg ccggaccccc ggccaaccgc ccctccccat acgctcactc
     4741 ctctccggct agcgcaccga gacggcggtg acgttttggt gccgctcgtc gggcacggcg
     4801 ccgggggacg gggcggggcg gcgcttgcgc acctcccgcc gggcgccgcc gatcaggtgg
     4861 ccggcgacga agtagagcaa cccaccggcg atcagcagcg cgaccagagc cacggcccct
     4921 tccagaatgg cggtcaacag ctcgtgaaca cctctcatag agcaaccctc cagcgctcgg
     4981 cgtcctgccg cttcaattcc agtccgaagc cggggcggcc gcggtcgggc cgcagcgcgc
     5041 cgtcgaccgg ctcgagcacg ccgtcgaaga ggaggtgcgc gatgcgcacg tggtcgtaga
     5101 aatactcgat gttcagcatc gaaggcagtg agcagcccgg tgaggcgtgc aagaacggcg
     5161 cgcaatgcgc cgagagcggc aggaagtgcg cctcgcagag cgcaccggtg cgcaggaagg
     5221 cggtcacgcc gccgcagcgg gtgacgtcgg cctggagcac atccacggcg ccggcctcca
     5281 gcatgcggcg gaaatgccac aggtcgtagc cgtactcgcc ggccgcgatg cgcagcggcg
     5341 ccggggcgcg atcgcgcagc agccgcaggc cgtcgaggtc gtggtggtag accggctcct
     5401 cgaaccagtc gacgccgagc tcggcgaagc gctcggcc
//
LOCUS       DASUBI010000085         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_911846, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000085 DASUBI010000000
VERSION     DASUBI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911846"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFU72_01620"
     CDS             complement(<1..942)
                     /locus_tag="VFU72_01620"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5422213.1"
                     /translation="MYDDSAPRLHTPLCDLLGIRYPIIQAPMAGGPTTPELIAAVSNA
                     GGLGMLGAARMPLDRLRATIHAIRALTDRPFGVNFLLAGPDTEPSAEEIAVVRQVLDR
                     FRAELGLPPGPAELALPPSSLDAAVDLTLEEHVPVLSSGLGDPAPWASEAHNSGARVL
                     AMVASVEEAVQVVAGGADVVVAQGAEAGGHRSSFVLGPGGEVPLVGTLALTPQVADAV
                     AAPVVAAGGIVDGRGVAAALALGASGAQLGTRFLLAREAGTAPAHQQRLLAAVETETA
                     VTRAFSGRPARSLRNRFSEEFAQSGAEPLPYLLQGMAA"
     gene            complement(1033..>2547)
                     /locus_tag="VFU72_01625"
     CDS             complement(1033..>2547)
                     /locus_tag="VFU72_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5422214.1"
                     /translation="RGSRLTAADLRRARTGAGPNPEQRAALPADHPAAHHRPPHSTPP
                     PMTRRRRLSPRRLLLLAVPLAACLVVAVYLGVLALRAQQAASKIFSLPRQPHPVVRVN
                     AQGTPEIDPQATAAAAGIPEWSKSGRVNLLLLGVDDRHDGEPARSDTMIVVSIDPSAK
                     TVGMLSIPRDLLVSIPGVGDDKINAAYPNGEAGSVGGAQLARATVEYNFQIPIDYVAT
                     VDFTGFQKIVDTLGGVTLDVATPLKDDEYPAENYNYTRVLFHSGVQHMNGQTALRYAR
                     TRHDDNDFARGDRQQQVLKALRAQAISLGLITKAPQLLSNLGDTFHTSMPVSDALSLA
                     KLATEIKGQNITSYSLLPDVSEQWTDAGYYLIPDWQKIQGTLHQMLGTAGSAATAGAP
                     TPTPTQTPDLSAAIAVQNATSINHLAANSAAILANAGFAAVTATQAEEPGSHPKSQIV
                     DYSGNPATARRIAALLGLPAAIVRQGDPGGGNGYDVVVTLGEDAPIPTPAAASP"
BASE COUNT          319 a          854 c         1012 g          362 t
ORIGIN      
        1 ggcggccatg ccctgcagca ggtaggggag cggctcggcg ccgctctggg cgaactcctc
       61 ggagaagcga ttgcgcaggc tgcgcgccgg gcggccactg aaggcgcgcg tgaccgcggt
      121 ctcggtctcg accgccgcca gcagccgctg ctggtgggcg ggcgcggtgc cggcctcgcg
      181 cgccagcagg aagcgggtgc cgagctgcgc cccgctcgcg cccagcgcga gcgccgccgc
      241 caccccgcgc ccgtcgacga tgccgccggc cgccaccacc ggcgcggcga cggcgtccgc
      301 cacctgcggc gtcagcgcca gggtgccgac cagcggcacc tcgccgcccg gcccgagcac
      361 gaagctggag cggtgaccgc cggcctcggc gccctgcgcc accaccacgt cggccccgcc
      421 ggccacgacc tgcaccgcct cctcgaccga ggccaccatc gccagcacgc gcgcgcccga
      481 gttgtgcgcc tcgctcgccc agggcgcggg gtcgcccagc ccggaactca gcaccggcac
      541 atgctcctcc agcgtcagat cgacggccgc gtcgagcgat gacggcggca gcgccagctc
      601 ggctggaccg ggcggcaggc ccagctcggc gcggaagcgg tcgagcacct ggcgcaccac
      661 ggcgatctcc tccgcgctcg gctcggtgtc cgggccggcc agcaggaagt tcaccccgaa
      721 ggggcggtcg gtcagcgcgc ggatggcgtg gatggtggcg cgcagtcgat ccagcggcat
      781 gcgcgcggcg cccagcatcc ccaggccgcc cgcgttggag acggcggcga tcagctcggg
      841 cgtggtcggc ccgccggcca tcggtgcctg gatgatcggg tagcggatgc cgagcagatc
      901 gcaaagcggg gtgtgcagcc ggggagcgga gtcgtcgtac atggccggtc tccttgtggt
      961 ggtgcgggcg ccggaagaca cgcttgacgc cgactctccg gcatcccggg ccggctgtca
     1021 acgcgccggc ttctacggcg aggcggcggc cggggtgggg atgggtgcgt cttcgccgag
     1081 cgtgacgacc acgtcgtagc cattaccccc gccgggatcg ccctggcgca cgatggcggc
     1141 gggcagtccg agcagcgcgg cgatacggcg ggcggtggcc gggttgccgc tgtagtcgac
     1201 gatctgcgac ttggggtggc tgcccggctc ctcggcctgc gtggcggtca cggcggcgaa
     1261 gcccgcattt gcgaggatcg cggccgagtt ggcggccagg tggttgatgc tggtggcgtt
     1321 ctgcaccgcg atggccgcgc tgagatcggg cgtctgcgtc ggggtgggtg tgggggcccc
     1381 ggcggtcgcc gccgaccccg ccgtacccag catctggtgc agcgtgccct ggatcttctg
     1441 ccagtcgggg atcaggtagt agccggcgtc ggtccactgc tcgctgacgt cgggcagcag
     1501 gctgtacgag gtgatgttct ggcccttgat ctcggtggcg agcttggcca gcgagagggc
     1561 gtcggagacc ggcatgctgg tgtggaaggt gtcgcccaga ttcgagagga gctggggcgc
     1621 cttggtgatc aagccgagcg agatagcctg ggcacgcagg gccttgagca cctgctgctg
     1681 gcggtcgccg cgggcgaagt cgttgtcgtc gtggcgcgtg cgggcgtagc gcagcgcggt
     1741 ctgcccgttc atgtgctgca cgccgctgtg gaagaggacg cgggtgtagt tgtagttctc
     1801 ggccgggtac tcgtcgtcct tgagcggcgt tgccacgtcg agcgtgaccc cgccgagggt
     1861 atcgacgatc ttctggaagc cggtgaaatc gaccgtggcg acgtagtcga tcgggatctg
     1921 gaagttgtac tcgacggtgg cccgggcgag ctgcgccccg ccgacgctgc cggcctcgcc
     1981 gttggggtag gcggcgttga tcttgtcgtc gccgacgccg gggatcgaga ccagcagatc
     2041 gcgggggatc gagagcatcc ccaccgtctt ggctgagggg tcgatgctga cgacgatcat
     2101 ggtatcggag cgggccggct cgccgtcgtg ccgatcgtcg acgccgagca gcagcaggtt
     2161 gacccggccg ctcttgctcc actcggggat gccggcggcg gcggccgtcg cctgggggtc
     2221 gatctccggc gtgccctggg cgttgacgcg caccaccggg tggggctggc gcggcagcga
     2281 gaagatcttg ctggcggcct gctgggcgcg cagcgccagc acgccgagat agaccgcgac
     2341 caccaggcag gcggccagcg gcacggcgag gagcagcagg cgccggggcg agagccgccg
     2401 ccggcgcgtc atcggcggtg gggtggagtg cggcggacga tggtgcgctg cggggtgatc
     2461 agctggcaag gccgctcgct gctcgggatt gggaccggcg ccagtgcggg cgcggcgcag
     2521 gtccgcggcc gtcaggcggg agccgcg
//
LOCUS       DASUBI010000086         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_378701, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000086 DASUBI010000000
VERSION     DASUBI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378701"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1157)
                     /locus_tag="VFU72_01630"
     CDS             complement(60..1157)
                     /locus_tag="VFU72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEU5422215.1"
                     /translation="MPLARPGRLRQLLSSAIAELHPGYFALVMATGIVAIALRQQGFR
                     PLDRWMFAVNLVAYPGLWLLTLARLLRARERVRADLLNPLRAPGFLTTVAATSVLGRQ
                     FVLMTDDTRVAFVLWLLSAALWLVLLYAFFAAMMLREHQPHAEQGLHGGWLLSVVATQ
                     SVALLGARVAERVTDWRETMLFWMLVLYLLGCLLYLLIGGLLLFRLLFLRPDPAALFP
                     LYWITMGAAAITVLTGSTLIEGAPEWPFLASLRPFLAGLTLLFWACASWWIPLLVILG
                     AWRHLVKRYPLVYEPQFWGLVFPLGMYTVASFETAAVLDVPALLGVAHIFVLVALAAW
                     VAVFAGMAHRVAGTLWLAFAPRRDALATEEP"
     gene            complement(1163..1816)
                     /locus_tag="VFU72_01635"
     CDS             complement(1163..1816)
                     /locus_tag="VFU72_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HEU5422216.1"
                     /translation="MTTPALDELLQASARLHAHLCPRQVLGVRMSLLAGWLLGLDLPR
                     RDKRLVVIVETDGCAADGVAVASGCWVGRRTLRVVDFGKVAATYCDSVSDRSIRIWPR
                     PEARLLAGRFAPETADRWQAQLLGYQRMPDDLLLDWATVVLDPPLETLISQPGLRVAC
                     AVCGEEIMNGREIDHEGVVMCRACAGQAYYRVAEEGGPAARAPGDHTRRCSPALPRP"
     gene            complement(1851..>2499)
                     /gene="narI"
                     /locus_tag="VFU72_01640"
     CDS             complement(1851..>2499)
                     /gene="narI"
                     /locus_tag="VFU72_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEU5422217.1"
                     /translation="PYVAIVLAVTVGLLRYYRDRYSYSSQSSQFLENRTLFWGSVAWH
                     YSILLILSAHVLALLFPGAWGSLIANQTRLYILEVTGLALALLTVLGLTLLIVRRIRF
                     PRVFAVTTILDWILLAALLGQVALGFTVALLYRWGSDWYLHTAVPWLVSLAVFDPKIS
                     YVTVLPWLVKLHMLGGFLIIALFPFTRLVHLVTFPVTYLWRPYQVVIWNARRTAR"
BASE COUNT          449 a          849 c          929 g          272 t
ORIGIN      
        1 gccgggcggg ctgccgggct cgcccgcgcc gcgtccgtga tgtcgcgcgc ctggaggtgt
       61 cacggctcct cggtagccag cgcgtcccgg cgcggcgcga acgcgagcca cagcgtgccg
      121 gcgacacggt gggccatgcc ggcgaacacc gcgacccagg ccgcgagcgc caccaagacg
      181 aagatgtggg cgacgccgag cagcgcgggg acgtcgagca cggccgccgt ctcgaagctg
      241 gccacggtgt acatgccgag cgggaagacc aggccccaga actgcggctc gtagaccagc
      301 ggatagcgct tcaccaggtg gcgccaggcg ccgaggatga ccagcagcgg aatccaccag
      361 ctcgcgcagg cccagaagag gagcgtgagc ccggccagga acggccggag gcttgccagg
      421 aagggccact ccggcgcgcc ctcgatcagc gtgctgccgg tgagcaccgt gatcgcggcg
      481 gcgcccatgg tgatccagta gagcgggaag agcgcggcgg ggtccgggcg cagaaagagc
      541 agccggaaga gcagcagccc accgatgagc aggtagagga gacagccgag caggtagagg
      601 accagcatcc agaacagcat cgtctcgcgc cagtcggtga cccgctcggc gacgcgcgcc
      661 ccgagcagcg ccaccgactg cgtcgccacc acgctgagca gccagccgcc gtgcagcccc
      721 tgctcggcgt gcggctgatg ctcgcgcagc atcatggcgg cgaagaaggc gtagagcagg
      781 acaagccaga gcgcggcgct cagcagccag agcacgaagg cgacccgggt gtcgtcggtc
      841 atcagcacga actggcggcc gagcacgctg gtggcggcca cggtggtcag gaaacccggc
      901 gcgcgcaggg ggttgagcag gtccgcgcgc acgcgctcgc gggcgcgcag cagccgggcc
      961 agcgtcagga gccagaggcc ggggtaggcg accaggttta cggcgaacat ccagcggtcg
     1021 agcgggcgga atccctgctg ccgcagcgcg atcgcgacga tgccggtcgc catcaccagc
     1081 gcgaagtagc ccgggtgcag ttcggcgatc gccgacgaga gcagctgccg gaggcgcccc
     1141 ggccgcgcga gcggcacgcc gatcatggcc gtgggagcgc gggggagcag cgtcgcgtgt
     1201 ggtcgccggg cgcgcgggcg gccgggcctc cctcctcggc cacccggtaa tacgcctgac
     1261 cggcacaggc gcgacacatg acgacgccct cgtggtcgat ctcacgcccg ttcatgatct
     1321 cctcgccgca gaccgcgcag gccacgcgca gccccggctg gctgatcagc gtctccagtg
     1381 gcgggtccag caccaccgtc gcccagtcca gcaggaggtc gtccggcatg cgctggtagc
     1441 cgagcaactg cgcctgccag cgatcggcgg tctccggcgc gaagcggccg gccagcaagc
     1501 gggcctcggg gcggggccag atccggatcg agcggtccga cacgctgtcg cagtaggtcg
     1561 ccgcgacctt gccgaagtcg accacgcgca gggtgcgccg gcccacccag cagccggagg
     1621 cgaccgccac accgtcggcg gcgcagccgt cggtctcgac gatcacgacc aggcgcttgt
     1681 cgcgtcgcgg caggtcgagg ccgagcagcc acccggccag caggctcatg cgcaccccga
     1741 gcacctggcg cgggcagaga tgggcgtgca gccgggcgct tgcctgcagt aactcgtcga
     1801 gcgcgggtgt cgtcacggtt tccgctccac tctggcgctc ggcgcggcgc ctagcgcgcc
     1861 gttcgccggg cgttccagat cacgacctgg tacggccgcc agagataggt gactgggaag
     1921 gtcaccaggt gcaccagccg ggtaaagggg aagagggcga tgatcaggaa tccccccagc
     1981 atgtggagct tgaccagcca gggcagcacc gtgacgtagc tgatcttggg gtcgaagacc
     2041 gccagcgaga cgagccaggg gacggcggtg tgcaggtacc agtccgaccc ccagcgatac
     2101 agcagcgcca ccgtgaagcc cagcgccacc tgcccgagca gcgcggccag caggatccag
     2161 tcgaggatgg tggtgaccgc gaagacgcgc gggaagcgga tgcgccgcac gatcagcagc
     2221 gtcagcccga gcacggtcag cagggcgagg gccagtcccg tcacctccag gatatagagg
     2281 cgggtttggt tggcgatcag cgagccccag gcgcccggga agagcagcgc cagcacgtgc
     2341 gcggagagga tcaggagaat gctgtagtgc caggcgaccg agccccagaa gagcgtgcga
     2401 ttctccagga actgcgacga ctggctggag tacgagtagc gatcccggta gtagcgcagc
     2461 aggccgacgg tgaccgccag cacgatcgcc acgtacggg
//
LOCUS       DASUBI010000087         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1250195, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000087 DASUBI010000000
VERSION     DASUBI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250195"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..843)
                     /locus_tag="VFU72_01645"
     CDS             complement(298..843)
                     /locus_tag="VFU72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU5422218.1"
                     /translation="MRQRLAGKTIGVFVAPEFEDLEFWVPVMRLREEGARVRVIGVNH
                     ETCTGKHCLEATPDAAASEVRAEDLFGLVIPGGWAPDKLRRDPDVLRLVRECHAAGKT
                     IGVICHAGWVAASAKIVNGMRFTGSRGIKDDLENAGATWVDEPAFREGNIVWGRVVDD
                     IPDFCRELVAAFVEQAGNDEG"
     gene            1292..2317
                     /locus_tag="VFU72_01650"
     CDS             1292..2317
                     /locus_tag="VFU72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5422219.1"
                     /translation="MKLVSYVTDPNAPRDAGRGGVLLGNDIFDLQHLIRWAARTGTAN
                     PPAGNLDGPPTTILELLALGPEALEELGQAVETAQRADPNDLKLHSGLVYDLGAVPLR
                     APIPNPPTIRDFYTFEQHVKSARARRGAGMIPEWYELPVFYFSNTSEVYGPEEAIPYP
                     QHSQEIDFELEIACVIGREGKNIAAEEAPNYIAGYLIMNDWSARDLWRKEQPLNMGPA
                     KGKDFATSLGPWLVTPDELADYTIGSGADQRLNLSMTARINSDMLSTDNAKNMYYSFP
                     QIIQQASMHARLRPGDVIGSGTCGTGCILELGTERHRWLQPGDTLELAIERLGMLRNT
                     IAAPEGK"
     gene            2325..2816
                     /locus_tag="VFU72_01655"
     CDS             2325..2816
                     /locus_tag="VFU72_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422220.1"
                     /translation="MPMPTPPAPGEEPTDESSVYRYHLRRLITWYLEALDPLPPEVFT
                     WHPPALQANTLAAICAHAIGSAEWWVLSCVGSGPIERDRDAEFAAEASWAELRPRFTA
                     FLAAAEELLAGMDSAALARISRHPAGDRMNRRCLLHTVEHLGLHLGHIEVTIDWWRVS
                     QAS"
     gene            complement(2928..>3683)
                     /locus_tag="VFU72_01660"
     CDS             complement(2928..>3683)
                     /locus_tag="VFU72_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422221.1"
                     /translation="PDPCRGDDHRYYSCLEHLYALSRVSDLLLVPFEPVRPELLGDTI
                     RNGEPWQPGAVVTEEERRGWWQALGMRPISEAQPFHSFYHEIVAVEQAEDPSEPISIT
                     TAIWSGFLLGQLLFARAGVAVRGGREHIVKEIAETSRLYWTYWRNNRHAVDDSHGWGS
                     NSQWGTDFRRDYVTAEAYHYNVDGTIPLLDDQGQVDPTALTEELSARGALEIMTHRCL
                     VSQHEGFWHVDLGEYTHREPRTPTPVAAHHNHG"
BASE COUNT          576 a         1392 c         1099 g          616 t
ORIGIN      
        1 cgagcggcgc ctgctcttgc gcgcgcgaac tctccagcac cagcgcccgc acacgcggct
       61 cctcacgcgc ccagcgctcc aggcgcgcga gcacctccgc ggcctccggc ctttcgctcg
      121 tcacgtcgcc cgccctcccg cactctttcg gagccagcgc cggcctcaag cggctccgct
      181 ggctctctcc ggctccctct ctattcgttc tccgcctctc tccctctttc tctccctccg
      241 tttcctctgc ggtaaattct cctccctccc tctctgtgcc ctctgtgtcc tccttgttca
      301 accctcgtcg ttccctgcct gctcgacgaa ggcggccacc agctcgcggc agaagtcggg
      361 aatgtcgtcc accacgcggc cccagacgat attgccctcg cggaaagccg gctcgtccac
      421 ccaggtcgcg cccgcgttct ccagatcgtc cttgatgccg cgcgagccgg tgaaccgcat
      481 gccgttgacg atcttggccg aggcggcgac ccatcccgcg tggcagatca cgccgatcgt
      541 cttgcccgcc gcgtggcact cgcgcaccag ccgcagcacg tccggatcgc ggcgcagctt
      601 gtccggcgcc cagccgccag gaatcaccag cccgaagagg tcctccgcgc gcacctcgct
      661 cgccgcagcg tcgggcgtcg cctccaggca gtgcttgcct gtgcaggtct cgtggttcac
      721 gccgatcacc cgcacccgtg cgccctcctc gcgcaggcgc atcaccggca cccagaactc
      781 cagatcctcg aactccggcg ccacgaagac gccaatggtc ttgcccgcca gccgttgtcg
      841 catggctcgc ctcctgtcgt ttctcggctc gctgccacta tcgctcgtgg tgtgcattgt
      901 cctcgatatt ctaccgcgct gggcaggtcc agccaccgaa ccgaaagggc ggccaccgga
      961 cgcccctacg agcacgtggc cgcgggaaca tccgaagcat ggctacagcg ccgcaagtcc
     1021 accgtgcacg gcagcggcgc acggtccttc gattgtccac tgtagcccca gtccgcggag
     1081 gcggacttcg cggccgcagg ccctccaggc gcggtttcca accgccggcc ccgcgccgtt
     1141 acgacgagca ttgcataacc ctgggcagtg gataagccgg cggccggtgt ctgtcgcgtg
     1201 caatgctata ctccgaagtg gcgcccgcga caccgctgtc cgccgcctca tctagatgta
     1261 tgccagcacg aagaagtgtc aggagcctgc catgaagctg gtcagctatg tcaccgaccc
     1321 caatgccccg cgcgacgccg gacgcggcgg cgtgctgctg ggcaacgata tcttcgacct
     1381 ccagcacttg attcgctggg cggcgcgcac cggcaccgcc aatccgcccg ccggcaacct
     1441 ggacggaccg cccaccacta tcctcgagct gctcgcgctc ggcccagaag cgctggagga
     1501 gctggggcaa gcggtcgaga ccgcgcagcg tgccgatccc aacgacctca agctgcactc
     1561 cggcctcgtc tacgaccttg gcgccgtgcc gctgcgcgcg ccgatcccca atccgccgac
     1621 gatccgcgac ttctacacct tcgagcagca cgtgaaatcc gcccgcgccc gccgcggcgc
     1681 cgggatgatt ccggagtggt acgagctgcc cgttttctac ttctccaaca cgagcgaggt
     1741 ctacggccct gaagaggcca tcccctaccc gcagcacagc caggagatag acttcgagct
     1801 cgagatcgcc tgcgtgatcg gccgcgaggg caagaacatc gccgccgagg aggcgcctaa
     1861 ttacatcgcc ggctatctca tcatgaacga ctggagcgcg cgcgacctct ggcgcaagga
     1921 gcagccgctc aacatgggtc ccgccaaggg caaagacttc gccacctcgc ttggcccctg
     1981 gctcgtcacc cccgatgagc tggccgacta caccatcggc tccggcgcgg accagcggct
     2041 caacctcagc atgaccgcgc gcatcaacag cgacatgctc tccaccgaca acgcgaagaa
     2101 catgtactac tcgttcccgc agatcatcca gcaggcctcg atgcacgccc gcctgcgccc
     2161 cggtgacgtg atcggctccg gcacctgcgg caccggctgc atcctggagc tgggcaccga
     2221 gcgccaccgc tggctgcagc ccggcgacac cctcgagctt gccatcgagc ggctgggcat
     2281 gctgcgcaac acgatcgccg cgccagaagg gaagtgacag actcatgccg atgccgacgc
     2341 cgcccgcacc cggagaagag ccgaccgacg agtccagcgt gtatcgctac cacctgcgcc
     2401 gcctgatcac ctggtatctg gaggcgctgg acccgctgcc accggaggtc ttcacctggc
     2461 acccgccggc cctccaggcc aacacgctgg ccgcgatctg cgcgcacgcc atcggctcgg
     2521 cggagtggtg ggtgctgagc tgtgtcggca gcggccccat cgagcgcgac cgcgacgccg
     2581 agttcgccgc cgaagcttcc tgggccgagc tgcgcccgcg ctttacggcc ttcctcgccg
     2641 ccgccgaaga gctcctcgcg ggcatggact ccgcggcgct ggcacgcatc agccgccacc
     2701 cggccggtga tcgcatgaac cgccgctgct tgctgcacac cgtcgagcat ctcggcctgc
     2761 atctaggcca tatcgaagtc acgatcgact ggtggcgcgt gtcgcaggcc agctaacaac
     2821 ctagcctctg cgtcctacac cttgcccgtt ggcctgggcc tcagtctctg cgctctgaca
     2881 ccatcgtcgc cttggtggtt gtagagtggt cagcgccggc ggccaaccta cccgtggttg
     2941 tggtgtgccg cgacaggcgt cggcgtgcgt ggctctcgat gcgtgtactc gcctaggtcg
     3001 acgtgccaaa acccttcgtg ctggctgacg aggcaacgat gcgtcataat ctcgagtgcg
     3061 cctcgtgctg agagttcctc ggtgagtgcc gtcgggtcga cctgcccctg gtcgtcgagc
     3121 aaggggatgg tcccgtccac gttgtagtgg tacgcctcgg cggtgacgta atcccgccgg
     3181 aagtcggtcc cccactgcga gttgctcccc cagccgtggg agtcgtccac ggcatgccgg
     3241 ttgttgcgcc agtaggtcca gtagagccgc gaggtctccg cgatctcctt gacgatgtgc
     3301 tcgcgccccc cgcgcacggc gacccctgcc cgcgcaaaca gcagctgccc cagcagaaag
     3361 ccgctccaga tcgcggtcgt aatgctgatc ggctcgcttg ggtcctcggc ctgctcgacg
     3421 gccacgatct catggtagaa ggagtggaag ggctgcgcct cagagatcgg gcgcatgccc
     3481 agcgcctgcc accagccacg ccgctcctcc tccgtgacca ccgcgccggg ctgccagggc
     3541 tcgccgttcc tgatcgtgtc acctaacagc tctggccgga ccggctcgaa tggcacgagc
     3601 aagaggtcgc tcacccgcga gagcgcgtag agatgctcca agcagctgta gtagcggtgg
     3661 tcgtcgcccc ggcagggatc agg
//
LOCUS       DASUBI010000088         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_214817, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000088 DASUBI010000000
VERSION     DASUBI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214817"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1062)
                     /locus_tag="VFU72_01665"
     CDS             complement(145..1062)
                     /locus_tag="VFU72_01665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5422222.1"
                     /translation="MKVGVNLLNYGPGASPASLLRWSRLAEALGYHFLMISDHVALTP
                     DVARSYPAPFYDPFLSLAWLAGRTRTVELGTTVIILPYRHPLQVARLASNLDQLSGGR
                     FIFGVGAGWAAQEFAALGLSVNERGAMTDEYLEVIQATWAGEVASYHGRFVAFDAVET
                     GPPPLQRPHPPLWIGGNGPAARRRAIRFDAAWHPISIRLPWLRDEALPDLRAQAAAAG
                     KPVPALCPRIRLHLTERPLDEAERRAGEGTLAQVRADLQELDELGASYVLLDTYRGDP
                     AGTLDHEHAWSMLATLAEQVLDLPNQRLR"
     gene            complement(1282..2022)
                     /locus_tag="VFU72_01670"
     CDS             complement(1282..2022)
                     /locus_tag="VFU72_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5422223.1"
                     /translation="MCFSDSARVPEPPIKGEVGQHGDLTLTASDGNKLMAYAARAGEP
                     RGIGLVILPDIRGLHQYYKDLSNAFAGTGIDTVAIDYFGRTAGHGERTDDWNYRPHVA
                     QTTPEGVALDTGAAVAYLRSSEGGKVEKIFTVGFCFGGSCSWNQAAAGHNLSGSIGFY
                     GRPERSEPFINEMQNPLLILIAGADRGIPGSAYVGFDHELNRAGVKHEMHTYAGAPHS
                     FFDRTFAEWQDACADAWQRMLGFIEQNA"
BASE COUNT          363 a          705 c          856 g          358 t
ORIGIN      
        1 cggttgcccc tgcgacaatg cgatggggac atagggtgac aagggaggcg aaggattggg
       61 tgggcaatag ggcagcgagg gcagacacgg aggtctgccc ctacatcggg cgaccaatgg
      121 cggacaacga tgaggcaaag cgcgctagcg caggcgctgg ttggggaggt cgagcacctg
      181 ctcagcgagg gtggccagca tggaccaggc gtgctcgtgg tcgagtgtgc cggccgggtc
      241 tccgcgatag gtgtcgagca ggacgtagct tgcgccaagt tcgtccagct cctggaggtc
      301 ggcgcgcacc tgtgcgagcg tcccttcgcc ggcccggcgc tcggcctcgt cgagcggccg
      361 ctcggtgagg tggagacgga tgcgcgggca gagcgcgggc accggcttgc cggccgcggc
      421 ggcctgggcg cgcaggtcgg ggagcgcctc gtcgcgcagc caggggagcc ggatggagat
      481 cgggtgccag gcggcgtcga agcggatggc gcggcgccgg gcggcagggc cgttgccgcc
      541 gatccagagc ggcggatgcg ggcgctggag cggaggcggg ccggtctcga ccgcgtcgaa
      601 ggcgacgaag cggccgtggt agctggcgac ctcgccggcc caggtcgcct ggatcacctc
      661 cagatactcg tcggtcatcg cgccgcgctc gttgaccgat aatcccagcg cggcgaactc
      721 ttgcgccgcc cagccggcgc ccacgccgaa gatgaagcgc ccgccgctca gttggtcgag
      781 gttgctggcc aggcgcgcga cctgcagcgg gtgccggtag gggaggatga tgacggtggt
      841 gcccagctcg accgtgcgcg tccgcccggc gagccaggcg aggctgagga aagggtcgta
      901 gaagggcgcg gggtagctgc gggccacgtc cggggtgagg gcgacgtggt cggagatcat
      961 caggaagtgg tagccgagcg cctcggccag ccggctccag cggagcagcg aggcgggact
     1021 cgctcccggg ccgtagttca gcaggttgac tccgactttc atcgggtgcc aatcacgagc
     1081 gggcctgagc gacgttcggt tctccggaca agagcatttc agccggctcc aacatccaga
     1141 gagatccttc gcggcggctc agagcctgcc ctgagcttgt cgaagggatg tcaggggaat
     1201 ggccgtcccc tccagtcatg ctgaggccgc agccgaagca tctccgtgat gcttgcgatg
     1261 ggcacggcct tggctgacgg gctacgcgtt ctgctcgatg aagccgagca tgcgctgcca
     1321 ggcgtcggcg caggcgtcct gccactcggc gaaggtgcgg tcgaagaagg agtgcggcgc
     1381 gcccgcgtag gtgtgcatct cgtgcttcac gccggcccga ttcaactcgt ggtcgaaccc
     1441 gacgtaggcc gagccaggga tgccgcggtc cgccccggcg atcaggatca agagcgggtt
     1501 ctgcatctcg ttgatgaagg gctcggagcg ctccgggcgg ccgtagaagc cgatcgagcc
     1561 ggagaggttg tggccggccg ctgcctggtt ccaggagcag gagccgccga agcagaagcc
     1621 gacggtgaag atcttctcga ccttaccgcc ctcggacgag cgcaggtagg cgaccgccgc
     1681 gccggtgtcg agcgccacgc cctcgggcgt cgtctgcgcc acgtgcgggc ggtagttcca
     1741 gtcgtcggtg cgctcgccgt ggccggcggt gcggccgaag tagtcgatgg cgacggtgtc
     1801 gatgccggtg ccggcgaagg cgttgctcag gtccttgtag tactggtgca gcccgcggat
     1861 gtcgggcagg atcacgaggc cgatgccgcg tggttcaccg gcgcgagcgg cgtaggccat
     1921 cagcttgttg ccgtcgctgg cggtcagcgt caggtcgccg tgctggccga cctcaccctt
     1981 gatcggtggc tcggggacac gcgcgctgtc ggagaagcac atcgtgggtc actccctccc
     2041 gttgaaccaa atgtcgcgtt tcaaacgcgg ccacaccctc atcgagaact gtaacatctc
     2101 cctccggcag tctattcgta tctatgccag cggagcgatg cgctgcgctg acggcgataa
     2161 gcgcttcacg tttgatggcc gcgcagaaac ccgaacacat gcccctgcaa ctgaccgatg
     2221 aagcgctgga aggacagatc atcttccgga aagctgatcg ccgtctccgg cgcgttcgtg
     2281 gc
//
LOCUS       DASUBI010000089         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_92764, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000089 DASUBI010000000
VERSION     DASUBI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92764"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFU72_01675"
     CDS             complement(<1..213)
                     /locus_tag="VFU72_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU5422224.1"
                     /translation="MNLGADIGLKMGKATRDAFGEALREAGKTNENIVVVDGDVNNST
                     RTEWFKEAFPERFFNVGIAESNLISVA"
     gene            complement(210..1100)
                     /locus_tag="VFU72_01680"
     CDS             complement(210..1100)
                     /locus_tag="VFU72_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5422225.1"
                     /translation="MADQQAKTQAPAAQGSGTNQSGGLSDQEVTRLRELSQTLRRQVL
                     EMTSEAGSGHPSSSFSCTELMAVLYFSGILKYDANNPHWPGRDRFILSKGHAAPILYA
                     VLANAGYFPVSELSTLRKLGSPLEGHPNMRRVPGVEASTGSLGQGLSMGEGHALAARW
                     APRDYNVYVLMGDGEQEEGQIWEAAMSSANFKLNNLIGIVDNNGFQQTTAVSNLTNPK
                     RYDDKYRAFGWQVAEIDGHDIRAVYQALTEATAYQDGPYCIIAHTVKGKGLEFMERDF
                     TWHGRAVPEKQLQKALEELS"
     gene            1295..2500
                     /locus_tag="VFU72_01685"
     CDS             1295..2500
                     /locus_tag="VFU72_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5422226.1"
                     /translation="MGKGRISRQRLALAVALLLSLASGITPRTASAANPAPAVVSFRV
                     YATQEGLVGESTSNGHTIQPNDHFVALPCVCALSSKGGHEFQVRLDYKGKSVTAPVWD
                     VGPWNIDDDYWNPPGQRKYQGLPQGVPEATAAYYNGYNGGRDGKGRQVRIPAGIDLAD
                     GTFADLGLTDSDWVTVSFLWLPAVSFSALPALPAGYQDIPTVAFGQRPPLDPATPKDP
                     AKFTYFPETQHNVAAPLMDYWNANGGWRNLGLPISELFREVYADGSWKLVQYFERQIL
                     ELNLPAKDGAPLVTSSLIGYSAYAPPDARAPVPAFTSDAAHQYFPQTQHSLNNGFKAY
                     WEAHGGLTAFGYPITEEFSGTTPDGRTYVAQIFERARFEWWPDRVGQPDEITQGLLVE
                     DLMRQAGWM"
     gene            2858..3535
                     /locus_tag="VFU72_01690"
     CDS             2858..3535
                     /locus_tag="VFU72_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5422227.1"
                     /translation="MVDIHLSPGEEDYLRAIYHLSQESQGEPVGTLALATWLEVASAS
                     VTNMVKKLAGRGLVDHSPYRGVTLTPLGRRAALEVVRHHRLLEQYLHEQLGMPWEQVH
                     VEADRLEHALSEDVEERIDRLLGHPTTDPHGAPIPSKSGTIARSDRRPLWLCAPETTV
                     VVAEVADEDADLLTYLDDLGLTPGTRVEVLAKAPFDGPLQVRIGQREYSLGERVARAV
                     QVTGATD"
BASE COUNT          562 a         1247 c         1151 g          590 t
ORIGIN      
        1 ggccacgctg atcaggttcg actcggcgat gccgacgttg aagaagcgct cggggaaggc
       61 ctccttgaac cactcggtgc gggtcgagtt gttgacgtcg ccgtcgacga ccacgatgtt
      121 ctcgttcgtc ttgcccgcct cgcgcagcgc ctcgccgaag gcgtcgcgcg tcgccttgcc
      181 catcttcaga ccgatgtccg ctcccaggtt catgacagct cctccagcgc cttctgcagc
      241 tgcttctccg gcaccgcgcg gccgtgccag gtgaagtccc gctccatgaa ttcgaggccc
      301 ttccccttca ccgtgtgcgc gatgatgcag taggggccat cctggtaggc ggtggcctcg
      361 gtcagcgcct ggtagacggc acgaatgtcg tggccgtcga tctccgccac ctgccagccg
      421 aaagcgcggt acttgtcgtc gtaccgcttg gggttggtca ggttggagac cgccgtcgtc
      481 tgctggaagc cgttgttgtc cacgatcccg atcaggttgt tgagcttgaa gttggccgat
      541 gacatggcgg cctcccagat ctggccctcc tcctgctcgc cgtcgcccat caggacgtag
      601 acgttgtagt cgcgcggcgc ccagcgcgcc gccaacgcgt ggccctcgcc catcgagagc
      661 ccctggccca agctgccggt cgaggcctcc acccccggca cgcgccgcat gttggggtgc
      721 ccctccaggg gactgccgag cttgcgcagc gtggacaact cgctgaccgg gaagtagccg
      781 gcgttggcca gcaccgcgta gaggatcggc gcggcgtgcc ccttcgagag gatgaagcga
      841 tcgcgccccg gccagtgggg gttgttcgcg tcgtacttga ggatgccgga gaagtagagc
      901 acggccatga gctcggtgca ggagaaggag ctgctggggt ggcctgagcc cgcctcgctg
      961 gtcatctcca gcacctgccg ccgcagcgtc tgcgacagct cccgcagccg cgtgacctcc
     1021 tggtcgctca ggccgccgga ctggttcgta ccgctcccct gtgcggccgg cgcctgggtc
     1081 tttgcctgct gatctgccat gcgacgaacc tccgtctgcg aattcgcgtg ctgtcgtcga
     1141 tcgctctgcc gcaagtggct caccaccatt cgactcccgg cacgcgcccg gcagtcgacg
     1201 gacggccgct cagacaaata ttgtcgcgcc gcccgccggc atggtacaac aggggagagc
     1261 cgaggagtgt acgtacactt acgagaggat gcccatgggg aagggaagga tctctcgaca
     1321 gcggctcgcg ctggccgtcg ccctgctgct cagcctggcg agcggcatca caccgcgtac
     1381 ggcgtcggcc gccaacccgg cgccagccgt ggtcagcttc cgcgtctatg cgacgcagga
     1441 gggcctggtc ggcgagagca cctccaacgg ccacaccatc caacccaacg accacttcgt
     1501 ggcgctcccc tgtgtctgcg ccctgagcag caagggcggg cacgagttcc aggtgcgcct
     1561 cgactacaag ggcaagagcg ttaccgctcc ggtttgggat gtcggcccct ggaacatcga
     1621 cgacgactac tggaacccgc cgggccagcg caagtaccag gggctgccgc agggcgtgcc
     1681 cgaggccacc gcggcctact acaacggcta caacggcggg cgcgacggga agggccgtca
     1741 agtgcgcatc ccggccggga tcgacctggc cgacggcacc ttcgccgacc tcggcctgac
     1801 cgactccgac tgggtcacgg tcagcttcct ctggctgccg gccgtctctt tcagcgccct
     1861 gccggcgctg cccgccggct accaggatat ccccacggtg gccttcggcc agcgcccgcc
     1921 gctcgacccg gccacgccga aggacccggc gaagttcacc tatttcccgg agacgcagca
     1981 caacgtggcc gcgccgctga tggactactg gaacgccaat ggcggctggc gcaacctcgg
     2041 gctgccgatc tcggagctct tccgtgaggt ctacgccgac ggctcctgga agctcgtgca
     2101 gtacttcgag cgccagatcc tggagctgaa cctgccggcc aaggacggcg cgccgctggt
     2161 gacctccagc ctgatcggct acagcgccta cgcgccgccc gacgcgcgcg ccccggtgcc
     2221 ggccttcacc agcgatgccg cgcaccagta cttcccgcag acgcagcatt cgctgaacaa
     2281 cggcttcaag gcctactggg aggcgcacgg cgggctgacc gccttcggct acccgatcac
     2341 cgaggagttc tccggcacca cgccggacgg gcgcacgtac gtggcgcaga tcttcgagcg
     2401 cgcccgcttc gagtggtggc ccgaccgcgt cggccagccc gacgagatca cccagggcct
     2461 gctggtcgag gacctgatgc gccaggccgg ctggatgtag gcgagccctc acgtatggag
     2521 gtcgctggcc gtcgaacact ttgggtgtcg ctctagcacg gatccttcga ctggctcagg
     2581 atgacagggt taagggagga gcctaccgga cgaggcaagt ccttgtcgtc cgcccctacc
     2641 taaccctgtc atcctgagct tgccgaagga tccgtcctca ccggacactc atcctcgtcg
     2701 ttcaccacac gacccttcaa ttccttcaac gtgcttgaca tcgcccgcgt ccggaggtaa
     2761 attagccatg gctaaacctc ggtttgtcga ccaagtcgat gcggattcgc cgcatcgaat
     2821 tgtttggcga aacactacct atagtctggg agcgatcgtg gtcgatatcc atctgtcgcc
     2881 gggtgaagag gattacctgc gcgccatcta ccatctgagc caggagagcc agggcgagcc
     2941 ggtcggcacg ctggcgctcg ccacctggct ggaggtcgcc tccgcctccg tgaccaacat
     3001 ggtcaagaag ctggccggtc gcgggctggt cgatcacagc ccctaccgtg gcgtgacgct
     3061 gacgccgctg ggccgccgcg ccgcgctgga ggtcgtgcgg caccatcgcc tcctcgagca
     3121 gtatctgcac gagcagctcg gcatgccctg ggagcaggtg cacgtcgagg ccgaccggct
     3181 ggagcacgcg ctctcggagg acgtcgagga acgcatcgac cggctgctcg ggcacccgac
     3241 gaccgacccc cacggcgcac cgatcccctc caaaagcgga acgatcgcgc gctcggaccg
     3301 ccggccgctc tggctctgcg cgcccgagac gaccgtcgtg gtggccgagg tggccgacga
     3361 ggacgccgac ctgctcacct atctcgacga tctggggctg acgccgggca cgcgcgtcga
     3421 ggtgctggcc aaggcgccct tcgacggccc gcttcaggtg cgcatcggcc agcgggagta
     3481 ctccctgggg gagcgtgtcg cgcgcgccgt ccaggtcacg ggcgcgacgg actagtcatg
     3541 aacggtgacg
//
LOCUS       DASUBI010000090         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1250107, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000090 DASUBI010000000
VERSION     DASUBI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250107"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..122)
                     /locus_tag="VFU72_01695"
     tRNA            complement(33..122)
                     /locus_tag="VFU72_01695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ser,seq:gga)
     gene            complement(130..220)
                     /locus_tag="VFU72_01700"
     tRNA            complement(130..220)
                     /locus_tag="VFU72_01700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:Ser,seq:cga)
     gene            complement(284..359)
                     /locus_tag="VFU72_01705"
     tRNA            complement(284..359)
                     /locus_tag="VFU72_01705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Arg,seq:gcg)
     gene            676..1686
                     /locus_tag="VFU72_01710"
     CDS             676..1686
                     /locus_tag="VFU72_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422228.1"
                     /translation="MVTSLVVRRLFFLVFVLIALSGVTFVLSHVVPSDPARMIAGPRA
                     SPQAVQKIRHDYGLDQPLAEQYVRYVTGVVRFNFGESISSQRPVSKDLKQYLPATVEL
                     TLYAMLFAVLVGVPLGVISATRRNSFIDGVGRVISVLGLSMPSFWLGLMLQFLLFAQL
                     GLLPDGQRLPIGVTPPHTITSLYTVDALLTGHVNLFFTALKHLVMPAFVLGLGSMAIV
                     TRMARAGMLEVLGQDYIRTAQSKGLGGRVIVMRHALKNALLPTITVIGMQIGLLMGGA
                     VLVEIIFSWPGIGRYAFQAIQTFDYNAVISVTLVIGFAYVVMNMLVDIAYLLLDPRIK
                     AT"
     gene            1683..2603
                     /gene="nikC"
                     /locus_tag="VFU72_01715"
     CDS             1683..2603
                     /gene="nikC"
                     /locus_tag="VFU72_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEU5422229.1"
                     /translation="MSIQDTAPRYSPAAQLSAEGSRVKSRTGLRYFLRSFSSDSPLNI
                     VALVIIAIFLLMSLLGPTLAPYDPIKPNILVKFHAPSTSHLFGTDELGRDVLSRVMSG
                     ARISLGIACLILSIAVTAGIIVGAIAGYVGGLVDELLMRVTDVFLAFPALILAMAIAA
                     TLGRDITNVVIALTVVYWPWYARLVRSQVIAIKERDYVEAARSVGARSRRILLRHILP
                     NSIAPVIIQLTIDVGYAVLATAGLSFLGLGAQPPSPEWGAMIAGARAYFRDAWWYITF
                     PGLALTFTVVAFNLLGDGLRDYLDPRTRGR"
     gene            2776..>3224
                     /locus_tag="VFU72_01720"
     CDS             2776..>3224
                     /locus_tag="VFU72_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422230.1"
                     /translation="MSDEHSGAYVELLLGATTGRLSRRAVLRRGLALGLATPLLLSLL
                     EACGSSSSSPTAGSGAGGTQPTASSAAGTSASPGAGGGKQSSNKQFVMALTGGVPDVD
                     PQSAYDNAASSLFFGTYEMLIRFKGESTSEFVPMLAKAWEQNADHTE"
BASE COUNT          510 a         1093 c          992 g          629 t
ORIGIN      
        1 cctggatccg ctgttctctc gtgttttggc ggcggaaggg gagggattcg aaccctcgag
       61 gcgctcaaca cgcctacgcg atttccagtc gcgcgcactc ggccaaacta tgcgaccctt
      121 ccatgctgtt ggcggagagg gtgggattcg aacccacggg acgctcatca cgcccaccga
      181 ttttcgagat cggcacctta aaccactcgg acacctctcc ccggtcttca gttgtcacag
      241 tcccgaacgc aagaatgcag ggtctggaac cctgcactgg gactggtacg cccggaggga
      301 cttgaacccc cgaccttttg gtccgcaacc aaacgctcta tccgctgagc tacgggcgca
      361 tcttggtttc gagcagcggc tacttgtcgt ccgccggcgc tccgaattct accatgagcc
      421 gccggaatcc ttcaaggaac cgccggtttg atcacgcaac gacatgttga cagcctcaac
      481 cgccgccggt tagagtttgg atcagtctca agagaccgac atgcacctgt caaatgccgc
      541 gtatgcgagg cgaaccgccg tttcgcgttg gtgcccgtca gggcctctgg cgcaggagac
      601 cgaaccccgg ccactccacc atcacatcct actccggccc tctccaatac ggcgcgaagc
      661 agagaaaggg gcgtgatggt taccagcctc gtcgtccgcc gcctgttctt cctggtcttc
      721 gttctgatcg cgctctccgg cgtgacgttc gtactctcgc acgtcgtgcc cagcgatccg
      781 gcgcgcatga tcgccgggcc gcgggccagc ccgcaggctg tccagaagat ccggcacgac
      841 tacgggctcg atcaacccct ggccgagcag tatgtgcgct acgtcacggg cgtcgtgcgc
      901 ttcaactttg gcgagtcgat ctcgtcgcag cggccggtct cgaaggatct caagcagtat
      961 ctgccggcga ccgtggagtt gacgctctac gccatgctct tcgcggttct cgtcggagtg
     1021 ccgctggggg tgatctccgc cacgcggcgc aattcgttca tcgatggggt tggccgcgtg
     1081 atctcggttc tcggcctctc gatgccgtcc ttctggctgg ggctgatgtt gcaattcctc
     1141 ctcttcgccc agctcgggct gctacccgac ggacaacggc tgccgatcgg cgtaacgccg
     1201 ccgcacacga tcacgtcgct ctacaccgtc gacgccttac tcaccggcca cgtcaatctc
     1261 ttcttcacgg cgctcaaaca cctggtcatg cccgcatttg tgctcgggct cggctcgatg
     1321 gcgattgtga cccgcatggc gcgcgccggc atgctcgaag tgctgggcca ggactacatt
     1381 cggaccgcac agtcgaaagg cctcggcggg cgcgtcatcg tgatgcgcca cgcgctcaag
     1441 aatgccctct tgccgaccat caccgtgatc gggatgcaga ttgggctgct gatgggcggc
     1501 gcggtgctgg tcgagatcat cttctcgtgg ccgggcatcg gccgctacgc gttccaggcg
     1561 atccagacct tcgactacaa cgcggtcatc tcggtcacgc tggtgatcgg cttcgcctac
     1621 gtggtgatga acatgctggt cgatattgcc tatctgttgc tcgatccaag gatcaaggcc
     1681 acatgagcat tcaagacacc gctccccgct acagtccggc cgcgcagctc agcgccgagg
     1741 gctcccgtgt taagagccgc actggcctgc gctatttcct gcgcagcttt tccagcgaca
     1801 gcccgctgaa catcgtggcc ctggtgatca tcgccatctt cctcttgatg tcgttgttgg
     1861 gccccaccct cgcaccatac gatccgatca agcccaatat cctcgtcaag ttccacgctc
     1921 cctcgaccag ccaccttttc gggacggacg aattgggccg cgatgtgctc agccgcgtca
     1981 tgagcggcgc ccgcatctcg ctcggcatcg cctgcttgat cctgtcgatc gccgtgacgg
     2041 cgggcatcat cgtcggcgcc atcgccggct acgtcggcgg gctggtcgat gagctgctga
     2101 tgcgcgtgac cgacgtgttc ctggctttcc cggcactgat cctggccatg gccatcgccg
     2161 ccacgctggg gcgcgatatc accaacgtgg tgatcgcact gacggtcgtc tattggccgt
     2221 ggtatgcccg cttggtgcgc agccaggtga tcgcgatcaa ggagcgcgac tacgtcgagg
     2281 ccgcgcgctc ggtcggggcg cgttcgcgcc gcatcctgct gcgccacatc ctgccgaact
     2341 cgatcgcgcc cgtcatcatc cagttgacca tcgacgtcgg ctatgccgtg ctggccaccg
     2401 cggggctctc gttcctcggc ctcggcgccc agccaccgtc gcccgagtgg ggcgcgatga
     2461 tcgccggcgc gcgcgcctat ttccgcgacg cctggtggta catcaccttc cccgggttgg
     2521 cgctgacctt cacggtcgtg gccttcaacc tcctcggcga cggcttgcgc gactatctcg
     2581 acccgcgtac gcgcgggagg taggcctgtc gatggagacg gtgagcgcac ggcgacgggc
     2641 ggttgcggga ggtgcctatg taatcggggc atagttggga gcggatctca ccaccggtcg
     2701 cccgatcgac cggcggacga gtccgtcgtg gttcagatgg ataacgagcc agtgtgagtg
     2761 agggggaaaa ggttcatgtc ggacgaacat tccggtgcct atgtcgagct gctgctcggc
     2821 gcgaccaccg ggcgtctctc gcgccgtgcc gtgctgcggc gcggcctggc cctggggctc
     2881 gcgacgcccc tgctcctgag cctcctcgag gcctgcggga gttccagcag cagccccacg
     2941 gcgggatcgg gtgcgggggg cacgcagccc accgcgagct ccgccgccgg caccagcgcc
     3001 agtcccggcg ccggaggcgg caagcagtcg agcaacaagc agttcgtgat ggcgctcacc
     3061 ggcggcgtgc cggatgtcga cccccagtcg gcctacgaca acgcggccag ctcgctcttc
     3121 ttcggcacct acgagatgct aatccgcttc aagggcgaga gcacctcgga gttcgtgccg
     3181 atgctggcca aggcctggga gcagaacgcc gaccatacgg agtt
//
LOCUS       DASUBI010000091         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_229227, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000091 DASUBI010000000
VERSION     DASUBI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229227"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1144
                     /locus_tag="VFU72_01725"
     CDS             92..1144
                     /locus_tag="VFU72_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5422231.1"
                     /translation="MPLLARMLATPLVIDVRPGAISGLGALLADRRIAVDGRVAVAVG
                     PGQGESIAAELDIGSAELFKVPDGSYDTAVALGKDLRAGTYEAVAGIGGGKTIDVTKF
                     AAHMAGIPMVSVATSLGHDGIASPVSTLAHESGSGSYGVVTPVAIVVDLDRVRRAPAR
                     LATAGIGDVVNDLSAIADWELSAADTGEPVDGLAVAMARSAAHAVLNLPGEAASDEFL
                     TVLAEALIMSGMSMIVAGSSRPASGGDHEIMHAINRLFPGTASHGELAGLGASFCAYL
                     RGDAAQLEAISSCLRRHGLPVHPEQVGLSAEQFTAAVVHAPQTRPGRYTILERLAMTP
                     AQAADRVEDYVSAIAG"
     gene            1173..1895
                     /locus_tag="VFU72_01730"
     CDS             1173..1895
                     /locus_tag="VFU72_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5422232.1"
                     /translation="MLDRLNDEHWFGRLYMRKLSPYATWVFARLGWSPNAVTLAYIAC
                     GIAAGVVIAFGGLLTAVIGAVLIQGYLLFDCSDGELARWSGRTSAGGIYLDLVGHYLG
                     ESALLVGLGLRAQGHLTVSGGYITAGVGAALLAMIVKAETDNVIVARAKQGLPAAHGD
                     AALAPTSSGLALARKLASLLRVHRITQAVELSVLVLAAAIIDTAVGGLIVTRFLLLAC
                     LVVAALMVIAHLVAIMASRRLR"
     gene            1892..2752
                     /locus_tag="VFU72_01735"
     CDS             1892..2752
                     /locus_tag="VFU72_01735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422233.1"
                     /translation="MKLSIVILTMGNRPDEFTRAIESTGPLRAAGAEVIVVGNGADPP
                     PVPAGVTTLTLAENAGVPGGKNAAVAACTGDVVMFLDDDGWYPEGAALGHHVTERFAG
                     DPSLAVLSFHIVDPEGGAGARWHVPRLRVGDPERASGVTTFLGGACAIRRSAYEEAGG
                     QPDRFFFGHEETDLSWRLIGLGYRIEYDPAARMCHPPVANARHAIWYRTEARNRVWLA
                     RRNLPWPLALAYLADWVALTVIRERSVSALRAWFAGFADGWRLDAGPRQPMSLRTVWR
                     LTKAGRPPIV"
BASE COUNT          387 a          947 c          991 g          442 t
ORIGIN      
        1 tcccgatcgg ggtcgtggac tgggtagagg tcgacaacca cgaggatctg ctgcgggcca
       61 ggcagatcgc cgcacgctgc taggaggatc gttgcccctg ctcgcgcgga tgctcgcgac
      121 gccgctcgtc atcgacgtgc ggcccggcgc catcagcggt ctcggcgcgc tgctcgccga
      181 ccgccggatc gccgtcgacg ggcgggtggc ggtcgcggtc ggccctggac agggtgagtc
      241 gatcgcggcg gagcttgaca tcggctcggc cgagctgttc aaggttcccg acggcagcta
      301 tgacaccgcc gtcgcgctcg gcaaagacct gcgagcgggc acttacgagg cggtggccgg
      361 catcggcggt ggcaagacca tagacgtcac caagttcgcc gcgcacatgg cgggcatccc
      421 gatggtctcg gtcgcgacga gcctcggcca tgacggcatc gcctcgccgg tcagcacgct
      481 ggcgcacgag tcgggcagcg gatcttacgg cgtggtgacg ccggtcgcca tcgtcgtgga
      541 cctcgaccgg gtgaggcgtg cccccgcgcg gctcgcgacg gcaggcatcg gagatgtcgt
      601 caacgatctg tcggccatcg ccgactggga actgtccgcg gccgacaccg gcgaacctgt
      661 cgacgggcta gccgtagcga tggcgaggtc ggccgcgcac gctgtactga acctgccggg
      721 cgaggccgca tcggacgaat tcctcaccgt cctcgccgag gcgctgatca tgtcggggat
      781 gtcgatgatc gtcgccggat cgagccgccc ggccagcggc ggcgaccacg agatcatgca
      841 cgcgatcaac cgactgttcc ccggtacggc gagccatggt gagctggcgg gacttggcgc
      901 ctcgttctgc gcctacctgc gcggcgacgc cgcgcagctc gaggcgatct cgtcctgcct
      961 gcggcgacac ggcctgccgg tgcatcctga gcaggtaggc ctgtcggccg agcagttcac
     1021 cgccgccgtg gtgcacgctc cacaaacgcg gcctggccgg tacacaattc ttgagcggct
     1081 cgcgatgacg ccagcgcagg ccgctgaccg agtggaggac tatgtcagcg ccatcgctgg
     1141 ctgagctgcg gtcaaaaggc cagccgcagg ctgtgctcga ccggctgaac gatgagcact
     1201 ggttcggccg cctctacatg cgcaagctgt cgccgtacgc cacctgggtg ttcgccaggc
     1261 tcggctggtc accgaacgcg gtcacgctcg cctacatcgc ctgcggaatc gcggccggag
     1321 tggtaatcgc gttcggcggg ctgctcaccg ccgtcattgg cgcggtgctg atccagggat
     1381 acctgctctt cgactgctct gacggcgagc tggccaggtg gtcgggtcgc acctcggccg
     1441 gcggcatcta cctggacctc gtcgggcact acctgggaga gagcgcgctg ctggtcgggc
     1501 tcgggctccg cgcgcaggga cacttgacgg tgtcgggcgg ctacatcacc gccggcgtcg
     1561 gcgccgcgct gctcgcgatg attgtcaagg ccgagaccga caacgtgatc gtggccaggg
     1621 ccaagcaagg gctgcctgcc gcccacggtg acgccgcgct cgccccgacc agctccggtc
     1681 tggcgctggc caggaagctc gcgagcctgt tgcgcgtcca ccggatcacg caggcggtcg
     1741 agctgtcggt gctcgtgctg gccgcggcga tcatcgacac cgccgtgggc ggcctcatcg
     1801 ttacccgctt cttgcttctg gcctgcctgg tcgtggcggc cctcatggtc atcgcgcacc
     1861 tagtggccat catggcgtcg aggcgattgc gttgaagctg tcgattgtga tcttgacgat
     1921 gggcaaccgg cccgatgagt tcacccgcgc gatcgagagc acggggccgc tcagggcggc
     1981 aggcgccgag gtgattgtcg taggaaatgg ggccgacccg ccgccggtgc ccgccggcgt
     2041 gaccacgctc acgctcgcag agaacgccgg cgtgcccggc ggcaagaacg cggcggtggc
     2101 ggcctgcacg ggcgacgtcg tgatgttcct cgacgatgac ggctggtatc ccgagggggc
     2161 cgcactcggc catcacgtga cggagcggtt cgccggcgac ccgtcgctgg cggtgctgtc
     2221 gttccacatc gtcgaccccg agggcggggc cggagcccgc tggcatgtgc cccgcctgcg
     2281 ggtcggcgac cctgagcgcg cctccggggt gaccaccttc ctcggcggtg cctgcgcgat
     2341 caggcggtca gcctacgagg aggccggcgg ccagcccgac cggttcttct tcggccacga
     2401 ggaaaccgac ctgtcgtggc ggctgatcgg gcttggctac cggatcgaat acgaccccgc
     2461 cgcgcggatg tgccacccgc cagtcgctaa cgccaggcac gccatctggt accggaccga
     2521 agcgcgtaac cgggtctggc tcgctcgcag gaacctgccg tggccgctgg cgctggcgta
     2581 cctggccgac tgggtcgctc tgaccgtgat cagggagcgc tcggtcagcg cgctacgcgc
     2641 gtggttcgcc ggcttcgccg atgggtggcg gctcgacgcc gggccgcgcc agccgatgtc
     2701 gcttcgcacg gtctggcgcc tgacgaaggc cggccgccct ccgatcgtct gagccgtgat
     2761 cacggag
//
LOCUS       DASUBI010000092         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1236995, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000092 DASUBI010000000
VERSION     DASUBI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236995"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1360)
                     /locus_tag="VFU72_01740"
     CDS             complement(356..1360)
                     /locus_tag="VFU72_01740"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5422234.1"
                     /translation="MGAHKVFGIGLSRTATMSLTSALKTLGFSAFHFPFDARTQQEIY
                     CYFDGAPTAPLELSILAEYDALTDIPVCCTYQGLDRAYPGSRFILTVRDKDAWLRSCE
                     RFWGQMVMPFIEANAGSDFERYLSFINTKVYGRVCFDRAAFAQVYDAYHAGVHAYFRD
                     RPDQLLTLDVTGGEGWEKLAPFLDRPIPDEPFPMENRLRLPQEATSDEAGRPAVVTGA
                     NPRIAWVQTYAGAFQARDLAACMAWYAPDALLELPNRQIRGYRAIEEWHREAFSSRGR
                     LDQLGRIEPTDDGVTTELTLSVSLLRVARIKIRGQLEVSFDGDDKITRVRFKFREFLR
                     "
     gene            complement(1571..2470)
                     /locus_tag="VFU72_01745"
     CDS             complement(1571..2470)
                     /locus_tag="VFU72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422235.1"
                     /translation="MRKQLAVGAVAVAGVVAARRLAPGRLKPRLRQLRTRVVYELLST
                     LVPESIAFTNYGYAALDGASGATLALAPEDEPQRYGMQLYHAVAGQVDLQGKDVLEVG
                     CGRGGGAAFVARYLAPRSVVGIDIAARAIAYARAHQQAEGLSFALGDAEHIPFPSASF
                     DAVVNVESSHSYPSMEGFLAEVRRVLRPGGALLFADMRARGDVARLRQQFEAAGFEVA
                     QEQPITAQVVRALELDSRRRVAMIEESVPKPLRRAAWEFTGAESTGNFRNLRDGRWEY
                     VRLVARKPAEPARETAGAAARSA"
     gene            complement(2700..>3524)
                     /locus_tag="VFU72_01750"
     CDS             complement(2700..>3524)
                     /locus_tag="VFU72_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422236.1"
                     /translation="LNWFMATRDAFGDSFYNFGARTMQASPSTMYLGSANAAQGTMVW
                     QVDGPAAAATSNALSSVEADVINQGTAITWDAALGATKYHIYRSDYVSNRAVQAVPAQ
                     QPVGDMNFVDAGIQPTQDIPGAPAPDIWLPGPFHEITTTTQTSFIDRSATPGGHYVYY
                     VQVEYSRGQLSQPSNLAPVPSQAPPVSFDSVVQQVRDLMSQGELSQYDGTRVLTWLSQ
                     AQTSANNGNLVMAQASLRRAEAIASNWRAGATDELVLNLQRLERQVRLSGQLQSGS"
BASE COUNT          546 a         1214 c         1205 g          559 t
ORIGIN      
        1 atgcggccgg ggacccatca ttggcggcgg cgctggcacc ctgactgagc agcaacgacg
       61 atagcagcag tgaaagcgtc agggccagcc gcgcgacgcc gcgagctcgg ccaaacgcca
      121 ccctccgagg accgcgcatg cgagactcct caaatccgaa tcccaaccca tgaccggcct
      181 gcgtcgcggc ggcgatcgag atacacgatc accgcgggtt cgcgccagcg tcgtccctcc
      241 tgccgatggg aacgtctggt cattgtatcg cgagcagggt ccgtcgcgat gctttcggca
      301 aaactttggt cctgtcccga cctatcctca cggatgctcg cgcccgctcg tcgtgctagc
      361 gcagaaactc gcggaacttg aaccgtaccc gcgtgatctt gtcgtcgccg tcgaagctga
      421 cctcgagctg gccgcgaatc ttgatgcgcg ccacgcgcag caggctgacg gagagcgtga
      481 gctccgtcgt cacgccatcg tcggtcggct cgatacgccc aagctggtcg agccgtccac
      541 gtgaggagaa cgcctcccga tgccactcct cgatggcccg gtagccgcga atctgccggt
      601 tgggaagctc caggagcgcg tcgggggcgt accacgccat gcaggcagcc agatcccgcg
      661 cctggaaagc accggcgtac gtctgcaccc aggcgatacg cggattggcg ccggtgacga
      721 cggccggccg gccggcttca tctgaggtgg cctcctgcgg cagccggagc cggttctcca
      781 tcgggaacgg ctcgtcggga atcggccggt cgaggaaggg ggccagcttc tcccagccct
      841 cgccgccggt cacgtcgagc gtcaggagct ggtccggcct gtcgcgaaag taggcgtgca
      901 cgccggcgtg atacgcgtcg tagacctgtg cgaaggccgc gcggtcgaag cagacgcggc
      961 cgtagacttt ggtgttgatg aagctcagat agcgctcgaa gtcggagccg gcgttggcct
     1021 cgatgaacgg catgaccatc tggccccaga agcgctcgca cgagcgcagc caggcgtctt
     1081 tgtcgcgcac ggtgaggatg aagcggctac cggggtaggc gcggtccagt ccctggtagg
     1141 tgcagcagac cgggatgtcg gtgagcgcgt cgtactcggc caggatcgag agctcgagcg
     1201 gggcggtggg cgcgccgtcg aagtagcagt agatctcctg ctgcgtgcgc gcgtcgaaag
     1261 ggaaatggaa ggccgagaag ccgagcgtct tcagcgcgct ggtcaggctc atcgtggcgg
     1321 tgcgactcaa accgatcccg aagactttgt gcgctcccac gctaccccca tgcgttgcta
     1381 gcccaacccg cggcgcccga gcgcgcggac gcacccggca agctaccgtg tttgacgttg
     1441 ccttgcggcg tcgtggccgc cgagtatacc aggcgagctt ctcgccctcc cactgctgat
     1501 cccgccggtc cgcggccaca tccaagctcg cgatgcggca ggagcgggcg gggcgcgctg
     1561 ctcgtcgggt tcaagcggac cgcgccgccg cgccggcggt ctcgcgcgcc ggctcagcgg
     1621 gtttgcgcgc caccaggcgg acgtactccc agcgcccgtc gcgcaggttg cgaaaattcc
     1681 cggtgctctc ggcgccggtg aactcccagg cggcccggcg cagcggcttc ggcaccgatt
     1741 cctcgatcat cgccactcgg cgccggctgt ccagctccag cgctcgcacc acctgcgcgg
     1801 tgatcggttg ttcctgggcc acttcgaagc cggctgcctc aaattgctgc cgcagccggg
     1861 cgacatcgcc gcgcgcgcgc atgtcggcga acagcagggc tccgcccggg cgcagcacgc
     1921 ggcggacctc ggccagaaag ccctccatgc tcggatagct gtgtgacgac tccacgttga
     1981 ccaccgcgtc gaacgaggcg gaggggaacg ggatgtgctc ggcgtcgccg agcgcgaacg
     2041 agagtccttc agcctgctgg tgagcgcggg cgtaggcgat ggcgcgcgcg gcgatgtcga
     2101 tgccgacgac gctgcgcggg gccagatagc gtgccacaaa ggcggccccg ccgccccgcc
     2161 cacaacccac ctccagcaca tccttgccct gcaaatcgac ctgaccggcc acggcatgat
     2221 agagctgcat cccgtagcgc tggggctcgt cttccggcgc gagcgcgagg gtcgctcccg
     2281 acgcgccgtc gagcgccgca tagccgtagt tggtaaacgc gatgctctcc ggtaccagcg
     2341 tgctcagcag ctcgtacacg acgcgtgtgc gaagctgtcg cagccgcggt ttgaggcgcc
     2401 cgggcgccag gcggcgcgcc gccacgactc cggcgacggc caccgctccg acggccagtt
     2461 gtttccgcat cccctcctcc tgcccgtagc cctcgtcgta cgcccatacc gcgccgtgag
     2521 gttgcggcag gggcccggcg ccgccggctt ggtccggcgg ccgcgactga aaggagggag
     2581 cgctaccgcc gcggcggcag cgctccgctc atccccacgt tcggtgatct cgtgttcccg
     2641 gctcccggcc acggtgcgcc aagacggttt gtcgcaccgg tcgtggccgg acgcgccggc
     2701 tagctacccg actgcaactg gcccgagagg cgaacctgcc gttccagccg ctgcaagttg
     2761 agcaccagtt cgtcggtcgc tccagcccgc cagttggacg cgatcgcctc cgcgcggcgc
     2821 agcgaggcct gcgccatgac caggttgccg ttgttggcgc tggtctgcgc ctggctcagc
     2881 caggtgagca cgcgcgtgcc gtcgtactgt gacagctcgc cctggctcat caggtcacgc
     2941 acctgctgga caacgctatc gaagctgacc ggcggcgcct gcgacggcac gggggccaga
     3001 ttcgacggct gggacaactg gccccggctg tactcgacct ggacgtagta gacgtaatgc
     3061 ccgccaggcg tggcgctgcg gtcgatgaag ctggtctgcg tggtggtcgt gatctcgtgg
     3121 aaggggccag gcagccagat atccggcgcc ggggcgccgg ggatatcctg cgtcggctga
     3181 atgcccgcgt ccacgaagtt catgtcgccc accggctgct gggccggcac ggcctgcacg
     3241 gcgcggttgg aaacatagtc cgagcgatag atgtggtatt tggtcgcgcc cagcgcggca
     3301 tcccaggtga tcgcggttcc ctggttgatc acatcggctt ccacgctgct cagcgcgttg
     3361 ctggtggcgg ccgcggccgg gccatcgacc tgccagacca tggtgccctg ggccgcgttc
     3421 gcgctgccga ggtacatcgt gctcggcgag gcctgcatgg tacgcgcgcc gaagttgtag
     3481 aagctatcgc caaaggcgtc gcgcgtggcc atgaaccaat tcaa
//
LOCUS       DASUBI010000093         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_501360, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000093 DASUBI010000000
VERSION     DASUBI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501360"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..824
                     /locus_tag="VFU72_01755"
     CDS             378..824
                     /locus_tag="VFU72_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422237.1"
                     /translation="MALTAKRRTGAVQKSVWLSPETIAAVGAWAEAQGATFSAALESL
                     ARVGLGQAAAEAVAPVVASAVRREVRAQVDRLASLLAAVAIDAGIGMRLGNATLRELA
                     PERVRTIAQAARLASVDALKRRRPARALGLLDGDDGDHEGELPQGE"
     gene            787..1233
                     /locus_tag="VFU72_01760"
     CDS             787..1233
                     /locus_tag="VFU72_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422238.1"
                     /translation="MAITKANYLKANSAGVRDRLARSIRYYTWRGSGERAGRRTQPRG
                     WVRDDGRALPWLAVRDCAGAEVAVYRYCYRIVLSTRETPLGFADYQAALGTRFARWYL
                     VQHHDGGHPHAHVIAFTERRLGRGDLAGQRARLLAREQSRAQSWGR"
     gene            1246..2526
                     /locus_tag="VFU72_01765"
     CDS             1246..2526
                     /locus_tag="VFU72_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU5422239.1"
                     /translation="MDWGDAQRMTARTGAVTWARDLLARDDWVVLDTETTGLGSAAAP
                     VRVAVVAPTGRVLLDTLVRPDRPVEDGAAAVHGLSGADLAEALAFGEVYPHLAQTVAG
                     REIVAYNAPFDRAVLDQACRAAGQSPLPARGWTCAMRQYAAWVGEWSERHGDWRWPRL
                     PGGNHTPVGDGRATLALVRRIAAGEPVEVTGQQSEEAFDDWQSTASDRGRPDSTPPAP
                     SGRIQALFTDPARFRDAEYQRWAQAQRTHDSLAASGRAGYQETARRYGPDFAAHILAE
                     YRRAHPTMPERAMLRVLADLGQEEGWDYYREYKLVPQPPPQPAPDDPRAGDGSGDRWY
                     GTPLYPDFAWPEQKLAVEVYGSAHDSDWLRRRGIVQREERRLATYAQEGWRVVTVTER
                     DLADRPEALRERLRRVLDEPPSLQPPLWVAATEH"
     gene            2547..2876
                     /locus_tag="VFU72_01770"
     CDS             2547..2876
                     /locus_tag="VFU72_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422240.1"
                     /translation="MSKDVALTVQVIDRTAWEGAGIVEPLGEPIPLAELLAQLPQHRL
                     ALRVLPASRIAGGAYAGGHVLIAQNGDRLYIAVPPAAIPQLAGVLPADEYAALVRRTP
                     AVRPDVH"
BASE COUNT          437 a         1090 c         1294 g          424 t
ORIGIN      
        1 gtgatcgcca tcgtccccgt ccaaaagacc cagcgcgcgc gctggcctcc gccgcttgag
       61 cgcatcgacg ctcgccagcc gcgccgcttg cgcgatcgcc cgtacccgct ccggcgccag
      121 ttcccggagt gccgcattcc ccagccgcat gccgatgccc ggcgtgccgg gcgcggtgcc
      181 ggggaggcat ccagggccgg attcgctcgc gcttatccgg tgcgcaagat aagggccgga
      241 tcgggccgca cggcggccct ggacgcctgg cggcgtcctc ccagcccatc ttgccagggg
      301 tgctggcgcc ccctggaccc ccgcccgtgg cgcgggtcgg ccagcggagg aacgagacag
      361 aggggaggga cgggctagtg gcgctgacgg cgaagcgccg cacgggcgcg gtgcagaaat
      421 cggtctggtt gtcccccgag acgatcgcgg cggtcggggc ctgggctgag gcgcaagggg
      481 cgaccttctc ggcggcgctg gagagcctgg cgcgcgtcgg gctgggacag gcggcggccg
      541 aggcggtcgc gccggtggtc gcgagcgcgg tgcggcgcga ggtgcgggcg caggtggacc
      601 ggctggccag tttgctggcg gcggtggcga tcgacgcggg gatcggcatg cggctgggga
      661 acgcgacgct ccgggaactg gcgccggagc gggtgcggac gatcgcgcag gcggcgcggc
      721 tggcgagcgt cgatgcgctc aagcggcgga ggccagcgcg cgcgctgggt cttttggacg
      781 gggacgatgg cgatcacgaa ggcgaactac ctcaaggcga atagcgctgg cgtgcgggac
      841 cgactggcac gctcgatccg ctactacacc tggcgcgggt cgggcgagcg ggcgggacgg
      901 cggacgcagc cgcgcggctg ggtgcgcgac gatgggcggg cgctgccctg gctggcagtg
      961 cgcgactgcg cgggagcgga agttgccgtc tatcgctact gctaccggat cgtcctctcc
     1021 acacgggaga cgccgctggg cttcgcagat taccaggcag cgctgggaac gcgcttcgcg
     1081 cgctggtatc tcgtgcagca ccacgatggg gggcatcccc atgcacacgt tatcgccttc
     1141 accgagcggc ggctagggcg gggggatctg gcggggcagc gcgcgcggct gctggcgcgg
     1201 gaacagtccc gcgcgcagag ctgggggcgc taggaggcca ggacgatgga ctggggcgac
     1261 gcccagagga tgacggcgcg gaccggtgcg gtgacctggg cgcgcgacct cctggcgcgg
     1321 gacgactggg tcgtcctcga tacggagacg accgggctgg gcagcgctgc cgcgccggtg
     1381 cgcgtggcag tggtcgcccc gaccgggcgg gtgctgctcg acacgctggt gcgacccgac
     1441 cgaccggtgg aggatggcgc tgcggcggtg catgggctca gcggcgctga cctggccgag
     1501 gctctggcct ttggtgaggt ctatccgcac ctagcgcaaa cggtggcggg gcgggagatc
     1561 gtcgcctata acgcaccctt cgaccgtgcc gtgctcgacc aggcgtgccg agcagccggc
     1621 caatctccgc tccccgcccg gggctggacc tgcgcgatgc gccagtacgc tgcctgggtc
     1681 ggcgagtgga gcgagcggca cggcgactgg cgctggcccc ggttgccagg cggcaaccac
     1741 acgccggtcg gcgatggtcg cgcgacgctg gcgctggtgc gccgaatcgc ggctggggag
     1801 ccggttgagg tgaccgggca gcagtctgag gaggcattcg atgactggca gagcacggca
     1861 agcgaccgag ggcggccaga ttctacgccc ccagcgccga gtgggcggat ccaggcgctc
     1921 ttcaccgacc ccgcgcgctt ccgcgatgcc gagtaccagc gctgggcgca ggcgcagcgg
     1981 acgcacgact cgctggcggc gagcgggcgc gcgggctatc aggagacggc gcggcgctat
     2041 ggccccgact tcgcggccca tatcctggcg gagtaccggc gcgcgcaccc gaccatgccc
     2101 gagcgggcca tgctgcgcgt gctggctgat ctcggccagg aggaggggtg ggactattac
     2161 cgggagtaca aactggtccc gcagccgcca ccgcagccgg cgccggacga cccgcgcgcg
     2221 ggagacggat cgggtgaccg ctggtatggc acgccactct atcccgactt cgcctggccg
     2281 gagcagaaac ttgcggtcga ggtctacggg agcgcgcacg actccgactg gctgcgccgc
     2341 cgggggattg tgcagcggga agaacgccgt ctggcgacct acgcccagga gggctggcga
     2401 gtggtgaccg tgacggagcg cgacctagct gaccgtcccg aggcgctgcg cgagcggttg
     2461 cggcgggtgc tggacgagcc gccatcgctg caaccgccgc tgtgggtggc ggcgacggaa
     2521 cactgatggt gggaaaggag ccggccatga gcaaggacgt ggcgctgacg gtccaggtga
     2581 tcgaccggac ggcctgggaa ggggccggta tcgtcgagcc gctcggcgaa ccgatccccc
     2641 tggccgaact cctcgcgcaa ttgccccagc accggttggc gctgcgggtg ctgccggcga
     2701 gccggatcgc cggtggggcg tatgccgggg ggcacgtcct aatagcacag aacggcgacc
     2761 gtctctatat cgcggtgccg ccggcggcta tcccccagct cgcgggggtg ctgccggcgg
     2821 acgagtacgc tgcgctggtg cggcggacac ccgccgtccg gccagacgtc cactgactca
     2881 gtgcagcgcg gccgccgcgc accgacgggg catcgccgcc ccaccggagc acgccggtag
     2941 ggcagtggtt gactgggttg gtggcgcacg ggcgctggcg cgcctgagcg cccggatggg
     3001 gtgcgtcccg gcggtcgcga tccgcggcac gctcgcgccg gcaccgacac cgccagtctg
     3061 gagccaccct cgcctcgccg tcgccccagg ctcgcacgca ccccgcgctc ggcaagggtg
     3121 cgcgctgcgc gccgcctccg ctctgctccg gccgcccttg ccggcgcgag gccccgcgcg
     3181 cggccgtggc tttgggcgag gcgagggtgg ctccagactg gtggcagtga cggaggttcg
     3241 ggcgt
//
LOCUS       DASUBI010000094         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_11281, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000094 DASUBI010000000
VERSION     DASUBI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11281"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFU72_01775"
     CDS             <1..221
                     /locus_tag="VFU72_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU5422241.1"
                     /translation="YGAVLGADALLVVTPWNEFKQADLERVSELMRAPVILDGRNIYD
                     QNEVTTLGFVYAGVGRRAPDLASAGFGS"
     gene            211..648
                     /gene="acpS"
                     /locus_tag="VFU72_01780"
     CDS             211..648
                     /gene="acpS"
                     /locus_tag="VFU72_01780"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5422242.1"
                     /translation="MAAEYQPNWDPERAGPIEVGIDAVEIARIRDALTRFGDRFLTRI
                     YTPRERERYGARPAELAARFAAKEAVMKALGTGVRGVRWRDIEVMPNARGKPVVVLYD
                     TARARAAALGLHHFAISLTHSRTEAIAMVVATTATPSTDAGAT"
     gene            645..2252
                     /locus_tag="VFU72_01785"
     CDS             645..2252
                     /locus_tag="VFU72_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5422243.1"
                     /translation="MRALCSVAQVRAAERAAIAGGAPEPELMLRAGSGVAELLAAVAH
                     SPGRALILVGPGNNGGDGLVAAARLAQLGWHCVIWSFRRPGVAGAPVDPAVVARFRWF
                     NAIEELRAAVTQADLVLDAVFGIGGRPGLPDAVVAAFTVVQEERRRRGVPLWALDIPS
                     GVDADTGAVAEGALIADATVMVGLAKLGLYRAPALRHAGRLWLVEIGLGEPDDAAVNP
                     EVVTPADAARLLPRRPRDAHKHGVGSLLVVGGAPNYYGAPRMAAEAALRAGAGLVALA
                     VPNSLIAPIATGLPETTFVPLPEDDAAVRADAVRGVMARYSALLIGPGLGRADATSEF
                     LARLFGFGNGAPADTAPFSSRAVIDADGLNWLAGRPEWPERLAGARLVLTPHPGEMAR
                     LSGRPLAEIEADPWRAAREAAERFGQVVVLKLAHAVVAAPGRRLVLAPQSEPALASAG
                     TGDVLAGTIAGLLAQGLDPWEAAVTGVMLGSAAAERAVARTGTLGLIAGDVIAELAPA
                     TRRLYDPHWRRMGEEDTWPSTWTWNGS"
     gene            2219..2701
                     /locus_tag="VFU72_01790"
     CDS             2219..2701
                     /locus_tag="VFU72_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5422244.1"
                     /translation="MAINLDVERIMTRDVLTVAPDTAVPAIAQLLWEHQISGVPVVED
                     GKVLGMVTEYDLIARESEYDAPLYVPFLDAYFRVPGTGDSRQRLNKILATTARELMTS
                     PPLVAAPNATVQEIATLLVDKRLTAVPIVDAHEQLVGIVSRSDIVRLMVVAEEIQDRE
                     "
     gene            complement(2712..3242)
                     /locus_tag="VFU72_01795"
     CDS             complement(2712..3242)
                     /locus_tag="VFU72_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEU5422245.1"
                     /translation="MVEVDRKSEQDVIDEALVVLNSQHHRLMRQLSPHAPENLTIEQL
                     RRSQLGRSLARLAAVARAEIREDKEQVLGVIDEVLSLLFTPPYGDDYTVPRSFWESDL
                     GRMLSMAKYRTYDSTELISIGNAAQQLGVTRPTIYRWMDDRMLGYVRDEMSGRTFVVR
                     EDVEALRQQGGGAEEQ"
     gene            3439..>4838
                     /locus_tag="VFU72_01800"
     CDS             3439..>4838
                     /locus_tag="VFU72_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422246.1"
                     /translation="MDILRRILGYLKPYVGHMVVGYLSLVLAIGMQLLTPRLVQYVID
                     QGLVSPNRHVVEVGALAIVGAGLVQGIFTFLRSYIFQFLAERVSFDVRNDLYRKMQGL
                     SFGYFDRANTGQLMSRATEDVNSIRRFLMQSLRSIVQTLGILAVITFILVRTDVTLAV
                     ISLSVMPLLLATALGFGHTIRPRFLKVQQQFGVMTTDLQENLAGARVVRAFAREGEES
                     QKFDNTLARLYDVEMATVRQYAFFFPLMTLLSSLGIALTLWYGGEQVVRGHLTLGQLT
                     AFYLYLSMLADPIRMLGWVVNSTARALASGERIFEVLDTRPEIQTPARPAPVAELDGE
                     VEFQQVGFRYPSAVEQALNDISFRATPGEKIALIGGTGSGKSSITALIPRFYDVSDGA
                     VMIDGHDVRTLALDMLRANVGMVLQDTFLFSVTIGENIAYGRPSASRDEIIAAAKAAR
                     IHDFITTLPQGYDTVVG"
BASE COUNT          696 a         1625 c         1693 g          824 t
ORIGIN      
        1 cctacggcgc ggtcctcggc gccgacgccc tgctggtcgt cacgccctgg aacgagttca
       61 agcaggccga cctcgagcgc gtgagcgagt tgatgcgagc gccggtgatc ctggatgggc
      121 ggaacatcta cgaccagaac gaggtcacga cgcttgggtt tgtctacgcc ggggtggggc
      181 gccgggcgcc cgatctggcc agcgccgggt ttggcagctg agtaccagcc gaactgggat
      241 ccggagcgtg ccggtccgat cgaagtcggt atcgacgcgg tcgagatcgc acgcatccgg
      301 gacgcgctga ctcgcttcgg tgaccgcttt ctgacgcgaa tctatacccc gcgcgagcgg
      361 gagcgctacg gcgcgcggcc ggccgagctg gccgcgcgct tcgcggccaa ggaggcggtg
      421 atgaaggcgc tgggaaccgg cgtgcgcggc gtccgctggc gcgacatcga ggtcatgcca
      481 aacgcgcgtg gcaagccggt ggtcgtgctc tacgacacgg ccagggcacg agccgcggcg
      541 ctcggcctgc atcacttcgc catctcgctg acgcactctc gcaccgaggc gatcgccatg
      601 gtggtggcca cgaccgcgac accgtccacg gacgcgggcg caacgtgagg gcactctgca
      661 gcgtggccca ggtgcgcgcg gccgagcgcg cggcgatcgc gggcggcgcg cccgagccgg
      721 agttgatgct gcgcgcgggc agcggcgttg ccgaattgct ggcagccgtc gcccattcac
      781 ccgggcgcgc gctgatcctg gtcggaccgg gcaacaacgg cggcgacggt ctggtggccg
      841 cggcgcggct ggcgcagctc ggctggcatt gtgtgatctg gtccttccgg cgcccaggcg
      901 tggcgggtgc gccggtcgat ccggcggtcg tcgcccgctt tcgctggttc aatgcgatcg
      961 aagagctacg cgccgccgtc acccaggccg atctggtgct cgacgcggtc ttcggcattg
     1021 gcgggcgtcc cgggctgccg gatgcagtcg tcgctgcctt caccgtggtc caggaggagc
     1081 gccggcggcg cggtgtgccg ctctgggcgc tcgacatccc ctcgggcgtc gatgccgaca
     1141 cgggagcggt cgccgagggc gccctgatcg cggacgcgac ggttatggtc ggattggcca
     1201 agctcgggct ctatcgcgcc ccggcgctgc gccacgccgg gcgcctgtgg ctggtcgaga
     1261 tcggcctcgg ggagccggac gacgccgccg tcaatccgga ggtcgtcacg cccgcggacg
     1321 ccgcccgcct gctcccgcgc cgcccgcgcg acgcacacaa gcacggggtc ggttcgctgc
     1381 tggtcgtcgg gggcgcgccc aactactatg gcgctccgcg gatggcggcc gaagcggcgc
     1441 tgcgcgccgg cgcggggttg gtggcgttgg cggtgcccaa ctcattgatc gcaccgatcg
     1501 ccaccggcct gccggagacc accttcgtac cgttgccgga ggatgacgcc gcggtgcggg
     1561 cggacgcggt gcgcggcgtc atggcgcgct acagcgccct gctgatcgga ccaggcctgg
     1621 ggcgcgcgga cgctacctcc gagttcctgg cgcgactctt cggcttcggc aacggtgcgc
     1681 cagccgatac ggctcctttc agctcccgcg cggtgatcga tgccgacggg ctcaactggc
     1741 tggcgggccg gccggagtgg cccgagcggc tcgccggcgc gaggctggtg ctgacgccac
     1801 atccgggcga gatggcccgg ctcagtggca ggccgcttgc cgagatcgag gcggatccct
     1861 ggcgggcggc gcgtgaggcc gccgagcgct tcggccaggt ggtggtgttg aagctggcgc
     1921 acgcggtcgt ggccgcgccg ggccgacggc tggtgctggc gccacagagc gagccggcgc
     1981 tggcctcggc cggcaccggc gacgtgctgg ccggcaccat tgccgggcta ctggcgcagg
     2041 ggctcgatcc ctgggaggcg gcggtcaccg gcgtgatgct cggctcggct gccgccgaac
     2101 gcgcggtcgc gcgcaccggg acgcttggtc tcatcgccgg cgacgtgatc gccgagctcg
     2161 cgccggccac gcgccggctg tacgatccac actggcgaag gatgggggag gaggacacat
     2221 ggccatcaac ctggacgtgg aacggatcat gacgcgcgac gtgctgaccg tcgcgccgga
     2281 cacggcggtt ccggcgatcg cccaattgct ctgggagcat cagatcagcg gggttccggt
     2341 ggtggaggac ggcaaggtgc tcggcatggt gaccgagtac gacctgatcg cgcgggagtc
     2401 ggagtacgat gcgccgctct acgtcccctt cctggacgcc tactttcgtg tgccgggaac
     2461 cggggatagc cggcagcgct tgaacaagat cttggcgacc acggcgcgcg agctgatgac
     2521 ctcaccgccg ctggtggcgg cgccgaacgc taccgtacag gagatcgcga cgctgctggt
     2581 cgacaagcga ctgacggccg tgccgatcgt cgacgcgcac gagcaactgg tcggtatcgt
     2641 cagccgatcc gacatcgtgc ggctgatggt ggtcgcggag gagattcagg accgggagta
     2701 acgacgcggc gctactgctc ctcggccccg ccgccctgct ggcgcagcgc ctcgacatcc
     2761 tcgcgcacca cgaaggtgcg cccggacatc tcgtcgcgca cgtagccgag catgcggtcg
     2821 tccatccaac ggtaaatcgt cggacgcgtg acgccgagct gctgcgccgc gttgccgatg
     2881 ctgatgagct ccgtcgagtc gtaggtgcgg tacttcgcca tcgagagcat gcggcccagg
     2941 tcggattccc agaagctacg cggcacggtg tagtcgtcac catagggggg cgtgaagagg
     3001 agggagagca cctcatcgat gacgcccagc acctgctcct tgtcctcgcg gatctcggcg
     3061 cgggcgaccg cggccaagcg cgccagactg cggcccagct ggctgcgcct gagctgctcg
     3121 atcgtgaggt tctctggcgc gtggggactg agttgccgca tcaggcggtg gtgctggctg
     3181 ttcagaacaa ccagcgcctc gtcgatgacg tcttgctcgc ttttgcgatc gacctctacc
     3241 attgccgcca ctccccaccg cgcaatgtcg aactcccaac ctcactgtac gtacctattg
     3301 tacgcgcggg cgcgcgtggg gtcaacaatg ttctcagccg gagccctgtc cagcggtcct
     3361 gaccagtcga acgctagtag tctggcgcgc gcggtgctat agtagagcgg tttgctcgcc
     3421 tcgggaagtg gagcgcctat ggacattctt cgccgcatcc tcggctattt gaagccgtat
     3481 gtcggccaca tggtcgttgg ctacctgtcc ctggtgctgg cgatcgggat gcaactgctg
     3541 acgccgcgcc tggtccagta tgtgatcgac cagggcttgg tgtcacctaa ccgccacgtc
     3601 gtcgaggtgg gcgcgctggc gatcgtcggc gccggtctcg tgcaaggtat ctttaccttt
     3661 cttcgaagct atattttcca gttcctcgct gagcgcgtct cgtttgatgt ccgcaatgat
     3721 ctctaccgga agatgcaggg gctctcgttc ggctacttcg accgcgccaa caccggccag
     3781 ttgatgtcgc gcgccaccga agacgtcaac tcgatccggc gcttcctgat gcagtcgctt
     3841 cgctcgatcg tccagacgct cggtatcctg gcggtgatca cttttatcct ggtgcgcacg
     3901 gacgtgacgc tggccgtcat ctcgttgagc gtgatgccgc tcctgctggc cacggccctc
     3961 ggcttcggcc acacgattcg cccccgcttc ctcaaggtcc agcagcagtt cggtgtcatg
     4021 accaccgacc tgcaggagaa cctggccggc gcccgcgtgg tgcgcgcctt cgcgcgcgag
     4081 ggtgaggaga gccagaagtt cgacaacacg ctggcacggc tctacgacgt cgagatggcc
     4141 accgtgcgcc agtacgcttt cttcttcccg ttgatgaccc tgctctcttc gctcggcatc
     4201 gcgctgaccc tctggtacgg cggcgagcag gtcgtgcgcg gccacctgac gttggggcag
     4261 ttgaccgcgt tctacctcta cctctcgatg ctggccgatc cgatccgcat gctcggctgg
     4321 gtcgtcaaca gcacggcccg cgcgctggcc tccggcgagc gcatcttcga ggtgctcgat
     4381 acccgtcccg aaattcagac tccggcgcgg ccggcgccgg ttgccgagct ggacggtgaa
     4441 gtcgagttcc agcaggtcgg cttccgctat cccagcgcgg tcgagcaggc gctgaacgac
     4501 atctccttcc gcgccacccc gggcgagaag atcgccttga tcggcggcac cggctccggt
     4561 aagagcagca tcaccgcgct gatcccgcgc ttttacgacg tcagtgatgg cgcggtgatg
     4621 atcgatgggc acgacgtgcg cacgctggcg ctcgacatgc tgcgcgccaa cgtcggcatg
     4681 gtgctgcagg ataccttcct cttttcggtc acgatcgggg agaacatcgc ctacggccga
     4741 ccaagcgcca gccgcgacga gatcatcgcg gccgccaaag cggcgcgcat ccacgacttc
     4801 atcaccaccc tgccgcaggg ctacgacacg gtcgtcgg
//
LOCUS       DASUBI010000095        15431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1073726, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000095 DASUBI010000000
VERSION     DASUBI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 15431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15431
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073726"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2019)
                     /locus_tag="VFU72_01805"
     CDS             complement(124..2019)
                     /locus_tag="VFU72_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5422247.1"
                     /translation="MDPRVTRYLPIKDYGLIGDCHSAALVSREGSIDWLCMPRFDSPS
                     LFARLLDYDKGGYWSIEPTDGFQATHQYVESTNVLETHLTTNQGEARILDFMPIRLKS
                     PSPTLWTAQSLYRVVEGLSGRVEIASVCAPRPEYASRRPSFQPEGGHVFFGDFLLIGP
                     GAWRIDPGHGAVSQRVTLRAGDRVAFGLHDIGDRSALTATPPDPFTSLESTLTYWKTW
                     ASHCTYTGPYREQVVRSALALRMLHYTPTGAIVAAPTTSLPEEIGGQRNWDYRFAWLR
                     DAAFTLYALLLAGYTEESGAFFSWILDVVKLDKTGLRILYPITRDGETVERTLDYLEG
                     YRASRPVRVGNGAVNQLQLDVFGEVFDALYFAWKVGKYDPHRVWTHFQPLADWVAKNW
                     QLPGSGIWEIRGGPRHFVYGKVMCWVALDRAIKLAEAGNLPGDVDHWRSERARIREAV
                     FTHGWSDRLQAFKQSFEDDCLDAANLRMSLVGFIDGTDPRMLSTIDAVQRHLLVDGLC
                     YRYLGIEDGVPGGESTFVLCTFWLINALLLAGRDAEAQDLYEQMLKQGTNLGLLSEEI
                     SVASGEFLGNYPQAFSQIGVISAAVSLAHEGQVGSVPAQPAEKAAGARLGDAHQGQKA
                     PAAAPRS"
     gene            2190..2684
                     /gene="purE"
                     /locus_tag="VFU72_01810"
     CDS             2190..2684
                     /gene="purE"
                     /locus_tag="VFU72_01810"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5422248.1"
                     /translation="MDALVGIIMGSQSDWETMRHAAETLDRLGVPYEVEIVSAHRTPD
                     RLFSYAGSALERGLEVIVAGAGGAAHLPGMTAAKTSLPVLGVPVQSRALNGLDSLLSI
                     VQMPAGVPVGTLAIGRAGAVNAALLAAAILGNKYPDVRLALERFRAEQTEAVLAEPRP
                     RDDA"
     gene            2681..3793
                     /locus_tag="VFU72_01815"
     CDS             2681..3793
                     /locus_tag="VFU72_01815"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU5422249.1"
                     /translation="MRVGIIGGGQLGRMLALAAYPLGIEVRTLEPAADAPAGRLTEQI
                     VAAYDDQEALASFAAGLDLVTYEFENVPVESAAFLAERLPVHPAPRALEVAQDRLHEK
                     TFFRSLGIPTPRFAPVASLTELRRAATEIGLPAILKTRRLGYDGKGQSVITREADVDV
                     AWAELGGEPLILEERLDFDRELSVLAARSTSGEFACYPLVENHHRGGILRLSLAPAPD
                     LDPALSEQAEALARHVLEEVGYAGLLAIELFERGGDLFANEMAPRVHNSGHWTIEGAE
                     TSQFEQHLRGILGLPLGSTAPRGHSAMINLIGELPPIEPILALRGAHLHLYQKAPRPG
                     RKLGHVTLCAGEPAELQRLLQSVTALVPEVPARSLI"
     gene            3848..6031
                     /gene="glgX"
                     /locus_tag="VFU72_01820"
     CDS             3848..6031
                     /gene="glgX"
                     /locus_tag="VFU72_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU5422250.1"
                     /translation="MPRRVLPGHPYPLGATWDGLGVNFAIFSENATRVELCLFDAADD
                     PHERDCVTFTERTGHVWHVYLGGVQPGQLYGYRVDGPYKPEEGLRFNPQKLLIDPYAK
                     ALHGAVNWDAKLFGYQLGDPQLDLSHSPDDDAFGVPKGVVVNPYFDWGEDRPPHIPWH
                     ESIIYEVHVKGFSVQNPNVPAEQRGTYSGLASPGAIEYFRSLGITAVELMPVHAFLDG
                     KHLIDQGLRNYWGYNTINYFSPDARYSSVGDIGAQVQEFKAMVKSLHRAGIEVILDVV
                     YNHTAEGNELGPTLSFRGIDNPAYYRLVPGNERYYMDYTGTGNSLNVRQPQVLKLIMD
                     SLRYWVEEMHVDGFRFDLAAALARELHDVDRLSAFFDIIHQDPVISRVKLIAEPWDVG
                     EGGYQVGNFPVLWTEWNGKYRDTIRKYWRGDESQLAELGYRLTGSSDLYQHDGRRPYA
                     SINFVTAHDGFTLHDLVSYDDKHNQANGENNHDGANDNWSSNYGVEGPTEDSAILAVR
                     ERQQRNFIATLLVSQGVPMICGGDEIGRTQQGNNNAYCQDNAISWYDWNLNDSERALF
                     EFTRRMIALRHHHPVLSRPRFFRGRPIRGSEIQDITWYRPDGNMMTAEEWDTAWMRCL
                     GMRLAGEDTGEVNEVGEPVVDDTLLIILNAAEEPVEFTLPDAHFGNAWEILVDTGQPE
                     RETKGQVVPYGEPLQLEPRSFVLMRWHLPINEAGFPGTPYHRVRG"
     gene            6316..8343
                     /locus_tag="VFU72_01825"
     CDS             6316..8343
                     /locus_tag="VFU72_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEU5422251.1"
                     /translation="MATNAKTEGQARALAPETRPRRVVIEGVQPEIDCGRFAAKRVVG
                     DLVAIEADIFTDGHDALAAVLRYRGPAADDWQETPFEPLVNDRWRATFRPAELGRWQY
                     AIEAWVDAFATWRRDFEKRAAAGVVEPVDLQTGAELIEAAAARAPGPEAAQLRRWASD
                     LRGDLALTERTVAALDPEMAALAGRFPDRSQSTTYHKDLTLTVDRERARFSSWYELFP
                     RSTGEQGRHGTFGSVEKRLPYIAAMGFDVLYLPPIHPIGSSHRKGRNNALVAETGEPG
                     SPWAIGGEAGGHRAIHPELGTVDELRHLIRAAAGYGLELALDIAFQASPNHPYVQEHP
                     AWFRWRPDDTIQYAENPPKKYEDIFPFDFESGDWRELWQELLGVVAYWIEQGIRIFRV
                     DNPHTKPFAFWEWLIGAIKAAHPEVIFLSEAFTRPKVMYRLAKLGFDQSYTYFAWRNA
                     KWELTEYFTELTRTEVVDYFRPNLWPNTPDILTEQLQTGLRPIFLMRLILAATLSASY
                     GIYGPAFELLEHQPRTPGGEEYLNSEKYEIRQWDLTRPESIADFIGLVNRIRRDNAAL
                     QANRSLRFHPVDNDQLIAFSKTSADRSNVVLTIVNLDPHYPQSGWLDLDLRALGLRPE
                     QSYSVVDLLTGEQFFWHGAHNFVMLNPQVLPAHVLAVRSQARDERDFEYYL"
     gene            8388..11723
                     /gene="treS"
                     /locus_tag="VFU72_01830"
     CDS             8388..11723
                     /gene="treS"
                     /locus_tag="VFU72_01830"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEU5422252.1"
                     /translation="MAQDDDATGIESNPIWYKDAIIYQLHPRAYGDSNADGIGDFRGL
                     IDKLDYIQDLGVTAIWLLPFYPSPGRDDGYDIADYTSVNPDYGSVADVRRLVREAHKR
                     GLRVITELVCNHTSDQHPWFQRARRAQAGSAWRDYYVWTDNPEKYKDARIIFKDFETS
                     NWSWDPEAGAYYWHRFYHHQPDLNFDNPRVHRAMFSTLDFWLRMGIDGLRLDAIPYLY
                     EREGTNCENLPETHEFLKLLRRHVDAEFDGRMLLAEANQWPEDAVAYFGDGDECHMSF
                     HFPLMPRMFMALRMEDRYPIVDIMAQTPEVPPTSQWALFLRNHDELTLEMVTDEERDY
                     MYRVYAHDPRMRINLGIRRRLAPLLGNDRRRIELLNGLLFSMPGTPVIYYGDEIGMGD
                     NIYLGDRNGVRTPMQWSADRNAGFSEAPRQRLYLPVIVDAEYHYEAVNVEAQQANPHS
                     LLWWMKRLIALRKQYRAFGRGSLEFLHPDNRRILAYIRQFQDEQILVVANLSRFVQAV
                     ELDLSAYQGLVPVELFGRNEFPQIGEQPYFLTLGPHSFFWFNLQPARVEVSQRAPGLE
                     SLPVLTVSGRWEHLLEGKGRRELARVLPGYLVDRRWFGGKARKIKSVSVTDSMPFPFD
                     ETLAFITLVQVDYVEGDSQTYLLPITYAPPDYAVELEALAPQALIARVRSRTHNVDGV
                     LFDATYDAAFDSALLAAVRGRRRLRGEGEVRALPLPALRDLLVLSEGELCPAPLRAEQ
                     SNSSICYDERLILKLFRRVEIGPNPDLEVSQFLTSHGFEHTPAVAGSIEYLSPDGKSA
                     ALAMLQGYVPNQGDAWEHTLGVLGRFFERAITEPIPDIPPSLEAERLILLAGQELPVI
                     AHDLTNGFLGDAELLGERTGEMHQTLARGQNAAFAPEPINSFSQRSIYQSMRAQAGLV
                     LPALRKQLGRMPAELRDDAEAVLEAEGRIQSAFRRVVSTKIEASRIRIHGDYHLGQVL
                     YTGRDFSIIDFEGEPARPLSERRHKRSPLIDVAGMLRSFHYATYFGLRQEQGSRVRPE
                     DVATLERWAGWWTAWVSSRFLSGYLRVTAGASFVPAEPAAFNALLDAFLINKAIYEVG
                     YELNNRPEWVSIPLKGLLRLIED"
     gene            11812..13758
                     /gene="glgB"
                     /locus_tag="VFU72_01835"
     CDS             11812..13758
                     /gene="glgB"
                     /locus_tag="VFU72_01835"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU5422253.1"
                     /translation="MATTLSRLTPNDLYLFNEGTHIRLYDKLGAHPTVVDGVAGVDFA
                     VWAPNARGVSVVGSFNGWNPEHDWLESLGNAGIWEGFIPGVGLGELYKFHIVGPNGQT
                     IEKADPFAFQAELPPATASVVCGLNYDWSDAEWMAGRGRRLAADAPVAIYEVHIGSWM
                     RVPEEGNRSLSYRELAPKLAEYVTRLGFTHVEFLPIMEHPFFGSWGYQITGFFAPDSR
                     YGTPQDFMYLIDYLHQHGIGVILDWVPSHFPVDVHGLASFDGTHLFEHANPMQGFHPD
                     WNSLIFNYGRHEVQAFLLSSIIFWLDRYHVDGIRVDAVASMLYLDYSRQPGEWIPNKY
                     GGRENLEAIAFLRTMNEVVYREFPDVQTIAEESTAWPMVSRPTYVGGLGFGMKWDMGW
                     MHDTLSYFSQDPIHRSYHHNRLTFRMIYAFNENFVLPLSHDEVVHGKGSLIGKLPGDD
                     WQKFANLRLLFAYMWAQPGKKLLFMGDEFGQWSEWNHDGQLQWDLLQYPAHQGIERWL
                     ADLNRAYHVEPALHRYDFDWAGFQWLDANDAPNSVLSFFRLSDKPEDRLLCVFNMTPV
                     PRYHYRVGVPLLGRWRELLNSDAEFYGGSGVGNFGGVDTTPIPSHGQPRSILLTLPPL
                     AALFFKHVPAEPPWEAATASPDDA"
     gene            13771..>15431
                     /gene="treY"
                     /locus_tag="VFU72_01840"
     CDS             13771..>15431
                     /gene="treY"
                     /locus_tag="VFU72_01840"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEU5422254.1"
                     /translation="MRDSHVPRATYRVQLTPEFGFAAARRLVPYLHALGVGDLYCSPI
                     LSARSGSRHGYDVTNPHEINPELGGRIEFDALTAALHEHGMGLLLDIVPNHMAASVEN
                     SWWADVLERGIASPYAGYFDIEWDSALANGVLQNRVLLPILGAPYGQTLENGELRLAL
                     EGDHIVLRYAETALPLSLASYRHILCWRLHALDASLAGEPELARAYHALLEQLGRLAA
                     EEETDPTARHHQRRALRPELHRLLGFPAIRAHLDHALAAINGRPGRPRSFDLLDRIIG
                     EQAYRLAFWELARERINYRRFFDVNDLVAIRVEEPDVFVATHQLFLELASQRQVSGLR
                     VDHIDGLRDPLGYLRRLRQALAEVGADDCYLVVEKILSPGESLPAGWPVAGTTGYDFL
                     NELGGLFVDDAGDAALDTIYRRWSGLVVDFPELAYQQKRRVLDDLFAGGVNELSLRLD
                     RIAESDRHGRDLPLASLRQALFELTASLTVYRTYIRDFAIAPDDRRWIERALADAARR
                     RPELSHALTFLRRILLLEFPANLPAEERSDRLDFVLSWQQFTGPV"
BASE COUNT         2602 a         5433 c         4880 g         2516 t
ORIGIN      
        1 acaacgagaa ccggctcaat ctccgtaaag tctccgttct ccacgagctt gccagccgcc
       61 gtgccggtgt tcgtagcctt tgcccgctct gcttcaagcg cgccgcatcc tcggtgttgc
      121 cgctcaggac cgcggagcgg cggccggcgc cttctgcccc tgatgcgcat cgccgaggcg
      181 cgcgccggca gccttctcgg cgggctgtgc cggcaccgag cccacctgac cctcgtgcgc
      241 cagcgagact gccgcgctga tcacgccgat ctgcgagaag gcctgcgggt agttgcccag
      301 gaactcgccg ctggccaccg agatctcctc cgagagcaga cccaggttgg ttccctgctt
      361 cagcatctgc tcgtacaggt cctgcgcctc ggcgtcgcgc ccggccagca gcagcgcgtt
      421 gatcagccag aaggtgcaga ggacgaacgt cgactcaccg cccggcaccc catcttcgat
      481 ccccaggtag cggtagcaca gcccatcgac cagcaggtgc cgctggaccg cgtcgatggt
      541 cgaaagcatg cgcgggtcag tgccgtcgat gaagcccacg agcgacatac gtagattggc
      601 cgcgtcgagg cagtcatcct cgaatgattg cttgaacgcc tgcagccgat cgctccagcc
      661 atgcgtgaag accgcctcgc ggatgcgcgc gcgctcggag cgccagtgat cgacatcgcc
      721 cggcagattt cccgcctcgg ccagcttgat cgcccgatcg agcgccaccc agcacatcac
      781 cttgccgtag acgaagtggc gcgggccgcc gcgaatctcc cagatacccg aaccgggcag
      841 ttgccagttc ttggcgaccc agtcggccaa cggctggaag tgtgtccaaa cgcgatgcgg
      901 gtcgtacttg cccaccttcc aggcgaagta gagggcgtcg aagacctcgc cgaagacgtc
      961 gagctgcagc tgattgaccg cgccgttgcc cacgcgtacc gggcgcgacg cgcgataacc
     1021 ctccaggtaa tcgagcgtgc gctcgacagt ctcgccgtcg cgcgtgatcg ggtagaggat
     1081 gcgcagcccg gtcttgtcga gcttgaccac gtccaggatc cagctgaaga aggccccgct
     1141 ctcctcggtg tagcccgcca acaggagtgc gtacagggtg aaggccgcgt cgcgcagcca
     1201 ggcgaagcgg tagtcccagt tgcgctgccc gccgatctcc tccggcagcg acgtggtggg
     1261 cgccgccacg atggcaccgg tcggcgtata gtgcagcatg cgcagcgcca gcgcgctccg
     1321 caccacctgc tcgcggtatg ggccggtgta ggtacagtgg ctggcccagg tcttccagta
     1381 ggtgagcgtg ctctccagac tggtgaaggg atcgggcggc gtcgcggtca gggctgaacg
     1441 atcgccgatg tcgtggaggc cgaaggccac gcgatcgccc gcccgcagcg tgacccgctg
     1501 cgaaaccgcc ccgtggcccg gatcgatccg ccaggcgccg ggcccgatca ggaggaagtc
     1561 tccgaagaag acatgcccgc cttcaggctg gaagctgggc cggcgggagg cgtactcggg
     1621 tcgaggagcg cagacgctgg cgatctcgac ccggcccgag agtccctcga ccacacggta
     1681 gaggctctgt gccgtccaga gcgtcggcga gggcgatttg aggcgaatgg gcatgaaatc
     1741 gaggatgcgc gcctcgccct ggttggtcgt gagatgcgtc tccagcacgt tggtgctctc
     1801 tacgtactgg tgcgtggcct ggaagccatc cgtgggctcg atgctccagt agccgccctt
     1861 gtcgtagtcg agcaggcgtg caaagagcga aggtgagtcg aagcgcggca tgcagagcca
     1921 gtcgatcgag ccttcgcggc tgaccagcgc tgccgagtgg cagtcgccga tcaagccata
     1981 gtccttgatc ggcaggtagc gtgtcacccg cgggtccatg cgtcctcctt cacggctacg
     2041 cgcactccgc gcgcatgggg gtcgatcgta tcacgaccaa ttgccgctct ggcatgtgca
     2101 aatccggtgc cgcatcggtt tggcgcactg tccgtgcata tgacatgatt cgcgcaggat
     2161 cggaaggcga tgtctgggga gtgaggagca tggacgccct ggtcggcatc atcatgggct
     2221 cgcaatcgga ctgggagacg atgcggcacg cggccgagac ccttgaccgg ctcggcgtgc
     2281 cctacgaggt cgagatcgtc tctgcacacc gcacccccga ccgcctcttc agctacgctg
     2341 ggtcagcgct cgagcgcggc ctggaggtga tcgtggccgg cgccggcggt gcggcccatt
     2401 tgcccggcat gaccgctgcc aagacgtccc tgccagtact cggcgtgcca gtgcagtcgc
     2461 gcgcgctcaa cggcctcgac tcgctcctct cgatcgtgca gatgccggcc ggtgtgccgg
     2521 tcggtacgct cgccatcggg cgcgcgggcg cggtcaacgc cgccctgctg gccgccgcga
     2581 tcctgggcaa caagtacccc gacgtgcggc tggcgctgga gcgcttccgc gccgagcaga
     2641 ccgaagccgt gttggccgaa ccgcgaccac gagacgacgc atgagagttg ggatcatcgg
     2701 cggtggacag ctcggccgca tgctggcgct ggctgcctac ccgctcggca tcgaggtccg
     2761 gacgctggag ccagcagcgg atgcgccggc cggccggctg accgagcaaa tcgtcgccgc
     2821 ctacgacgac caggaagcgc tggcctcctt cgccgccggg ctcgacctcg tcacctacga
     2881 gttcgagaac gtgccggtcg agagcgccgc cttcctggcc gaacgcctgc cggtgcaccc
     2941 cgcgccgcgt gcgctggagg tcgcccagga ccgcttgcac gagaagacgt tcttccgctc
     3001 gctcggcatc ccaaccccgc gcttcgctcc ggtcgccagc ctgaccgagc tcaggcgggc
     3061 cgcgaccgag atcggtctac ccgccatcct caagacgcgt cgcctcggct acgacggcaa
     3121 gggccagtcg gtcatcaccc gtgaggcgga cgtcgacgtc gcctgggccg agctcggcgg
     3181 cgagccgctc atcttggagg agcgactcga cttcgaccgg gagctctcgg tcctggccgc
     3241 gcgttcgacc agcggcgagt tcgcctgcta cccgttggtg gagaaccacc accgaggcgg
     3301 catcctgcgc ctctcactcg ctccggcgcc cgatctcgat ccggcgctga gcgagcaggc
     3361 cgaagcgctg gcccggcatg ttctggagga ggtgggctac gccgggctcc tggcgatcga
     3421 gctcttcgag cgtggtggcg atctgttcgc gaacgagatg gccccccgcg tccacaactc
     3481 gggccactgg acgatcgagg gcgcggagac cagccagttc gagcagcacc tgcgggggat
     3541 tttgggcctg ccactcggct cgacagcccc gcgcggccac tcggccatga tcaacctgat
     3601 cggtgagctg ccgccgatcg agccgatcct ggcgcttcgc ggcgcccacc tgcatctgta
     3661 ccagaaggcg ccgcgccccg gccgcaagct gggccacgtc acgttgtgcg cgggcgagcc
     3721 ggctgagctg caacggttgc ttcagtccgt caccgcgctc gtgcccgaag tgccggcacg
     3781 ctctttgatt tagcagccgg gcggggtgct ccccgcgcgt ccgttcatgt tgaaggggga
     3841 gattgcggtg ccgagaaggg tgctgcccgg ccatccctac ccgctagggg ccacctggga
     3901 cggactggga gtcaatttcg cgatcttctc cgagaacgcc acacgggtgg agttgtgtct
     3961 gttcgacgcg gccgacgatc cccacgagcg cgactgcgtc acgttcaccg agcgcaccgg
     4021 tcacgtctgg catgtctatc tcggcggcgt gcagccggga cagctctacg gctaccgcgt
     4081 ggacggtccg tacaagcccg aagagggcct gcgcttcaac ccgcaaaagc tcttgattga
     4141 tccctacgcc aaggcgttgc acggcgcggt caactgggat gccaaactct tcggctacca
     4201 gcttggcgat ccgcagctcg acctctcgca ctctcccgac gacgacgcct tcggcgtgcc
     4261 caagggggtc gtcgtcaacc cctacttcga ttggggcgag gaccggccgc cgcacatccc
     4321 ctggcacgag tcgatcatct acgaggtcca cgtcaagggc ttcagcgtcc agaatccgaa
     4381 cgtgccggcg gagcagcgcg gcacctactc cggcctcgcc tcgcccggcg ctatcgagta
     4441 cttccgctcc ctcggcatca ccgccgtcga actgatgccg gtgcacgcct tcctcgacgg
     4501 caagcacctg atcgaccagg gcctgcgcaa ctactggggc tacaacacca tcaactactt
     4561 ctcccctgac gcgcgctact ccagcgttgg cgacatcggc gcccaggtgc aagagttcaa
     4621 ggccatggtc aagtcgcttc atcgcgccgg catcgaagtt atcctcgacg tggtttataa
     4681 ccacaccgcc gaggggaatg agctcggccc cacactcagc tttcgcggca tcgacaaccc
     4741 tgcctattat cgcctcgtac ccggcaacga gcgctactac atggactaca ccggcaccgg
     4801 caacagtctc aacgtgcgtc agccgcaggt gctcaagctg atcatggact cgctgcgcta
     4861 ctgggtcgag gagatgcacg tcgacggctt ccgcttcgat ctggcggccg ccctggcgcg
     4921 cgagctgcac gacgtcgacc gcctgtcggc cttcttcgac atcatccacc aggacccggt
     4981 catctcgcgg gtcaagctga tcgcggagcc ctgggatgtg ggcgagggtg gctatcaggt
     5041 gggcaacttc cccgttctct ggaccgagtg gaacggcaag taccgcgaca cgatccgcaa
     5101 gtactggcgc ggcgacgaga gccaactggc cgagctgggc tatcgcctga ccggcagcag
     5161 cgatctctac cagcacgacg gccggcggcc gtacgccagc atcaacttcg tcaccgccca
     5221 cgacggcttc accctccacg acctcgtcag ctacgacgac aagcacaacc aggccaacgg
     5281 cgagaacaac cacgacggcg ccaacgacaa ctggtcaagt aactacggcg tggagggccc
     5341 gaccgaggat tccgccatcc tcgcggtgcg cgagcgccag cagcgcaact tcatcgcgac
     5401 cctgctcgtc tctcaggggg tgccgatgat ctgcggcggc gacgagatcg ggcgcaccca
     5461 gcagggcaac aacaacgcct actgccagga caacgccatt agctggtatg actggaattt
     5521 gaacgactcc gaacgcgcgc tcttcgagtt cacccgccgc atgatcgcgc tgcgccacca
     5581 ccatcccgtg ctgagccggc cgcgcttctt ccgtggccgc ccgatccgtg gctcggagat
     5641 ccaggacatc acctggtacc gtcccgacgg caacatgatg accgccgagg agtgggatac
     5701 ggcctggatg cgttgcctgg ggatgcggct ggccggcgag gataccgggg aagtcaacga
     5761 ggttggcgag ccggtcgtcg acgacacgct cctgatcatc ctcaacgcgg ccgaggagcc
     5821 ggtggaattc accctgcccg acgcacactt cggcaacgcc tgggagatcc tggtcgacac
     5881 gggccagccg gagcgtgaga cgaaaggaca ggtggtgccc tacggtgagc cgctgcagct
     5941 cgaaccgcgc tcgttcgtac tgatgcgctg gcacctgccg atcaacgaag ccggcttccc
     6001 cggcacgccc tatcaccgcg tgcgaggcta gaagctgacg aggagcacga gtgggtcgcc
     6061 aggacggatc cttcgacaag ctcaggatga cagtctaggg tttgaggcct tgcgggcgag
     6121 gcatatctcc ctcgtcagcc tctcccgtac ccctgtcatc ccttcggcaa gctcagggca
     6181 ggctctgggc gaagtcgaat gatccgtctt cacgcggcac catcgccatc gtcagccgcg
     6241 aacgagcgta cagcggcgct ggcaccggag gtgcgcacgg agcgccaacg gacatcggca
     6301 aagggggagc gacgagtggc aaccaacgcg aagaccgagg gccaggcccg tgcgctggcg
     6361 cccgagacgc ggccgcgccg cgtcgtgatc gagggtgtcc agcccgagat cgactgcggc
     6421 cgcttcgcgg ccaagcgcgt agtgggcgac ctggtcgcga tcgaggccga catcttcacc
     6481 gacggccacg acgccctggc agcggtgctg cgctaccgcg ggcccgccgc cgacgactgg
     6541 caggagacac ccttcgagcc gctggtaaac gaccgctggc gcgcgacctt tcgtccggct
     6601 gagttgggcc gctggcagta cgccatcgag gcctgggtcg acgccttcgc tacctggagg
     6661 cgcgatttcg aaaaacgtgc ggcggcgggg gtcgtcgagc ccgtcgatct ccagaccggc
     6721 gccgaactga tcgaggcggc cgccgctcgc gctcccggcc ccgaagccgc gcaactgcgg
     6781 cgctgggcca gtgatcttcg cggcgacctg gcgctgaccg agcgcaccgt ggcggcgctc
     6841 gatcccgaga tggcggcgct ggccggccga ttccccgatc ggagtcagtc caccacctac
     6901 cacaaggatc tgacgctgac tgtcgaccgc gaacgggccc gtttcagctc ctggtacgag
     6961 ctcttcccgc gctccaccgg cgagcagggc cggcacggca ccttcggcag cgtcgagaaa
     7021 cgcctcccct acatcgccgc catgggcttc gacgtgctct acctgccgcc gatccacccc
     7081 atcggtagca gccatcgcaa ggggcgcaac aacgccctgg tggccgagac aggcgagccg
     7141 ggcagcccgt gggccatcgg cggcgaagcc ggcggccacc gcgccattca cccggaactc
     7201 ggcaccgtcg acgagctccg ccatctgatc cgggccgctg ccggctacgg tctcgagctg
     7261 gcgctcgaca tcgcgttcca ggcctcgccc aaccacccct acgtccagga gcacccggcc
     7321 tggtttcgct ggcgcccgga cgacacgatc cagtacgccg agaacccgcc caagaagtac
     7381 gaggatattt ttcccttcga tttcgagtcg ggcgactggc gcgagctgtg gcaagaactc
     7441 ctgggcgtgg tcgcctactg gatcgagcag ggcatccgca tcttccgggt cgacaatccg
     7501 cacaccaagc cgttcgcctt ctgggagtgg ctaatcggcg cgatcaaggc cgcgcatccc
     7561 gaggtcatct tcctctcgga agccttcacc cggccgaagg tgatgtaccg gctggccaag
     7621 ctcggtttcg accagtccta tacctacttc gcctggcgca acgcgaagtg ggagctgacc
     7681 gagtacttca ccgagctgac caggaccgag gtcgtggact attttcggcc gaacctctgg
     7741 cccaacacgc ccgacatcct gaccgagcag ctgcagaccg ggctgcggcc gatctttctg
     7801 atgcgcttga tcctggccgc caccctctcg gccagctacg gcatctacgg tccggccttc
     7861 gaactgctcg agcaccagcc acgtacgccc ggcggcgagg agtacttgaa ctccgagaaa
     7921 tacgagattc gccagtggga tctgacgcgg cccgagagca tcgccgactt catcggcctg
     7981 gtgaaccgca tccggcgcga taacgccgcc ttgcaggcca atcgcagcct gcgcttccat
     8041 ccggtcgaca acgatcaact gattgccttc agtaagacca gcgccgaccg gagcaacgtc
     8101 gtgctgacga tcgtcaatct cgatccgcac tacccgcaat ccggatggct cgacctcgat
     8161 ctgagagccc tcgggctccg cccggagcag tcgtattcgg tcgtcgatct cttgaccggc
     8221 gagcaattct tctggcacgg cgcgcacaat tttgtcatgc tcaacccaca agtcttgccg
     8281 gcccacgtgc tggccgtgcg cagccaggcg cgggacgagc gtgacttcga gtactacctc
     8341 tgacacccgc taccggacac cgacaggaag ggagcaggac gccaatcgtg gcccaggatg
     8401 acgacgcgac cggcatcgag agcaacccga tctggtacaa ggacgccatc atctaccaat
     8461 tgcatccccg agcctacggc gacagcaacg ccgacggcat cggcgacttc cgcgggctga
     8521 tcgacaagct cgactacatc caggacctcg gggtcaccgc catctggctc ctccccttct
     8581 atccctcgcc cgggcgagac gacggctacg acatcgccga ctacaccagc gtcaaccccg
     8641 attacggcag cgtggccgac gtgcgccggc tggtgcgcga ggcgcacaag cgcggcctgc
     8701 gcgtcatcac cgagctggtc tgcaaccaca cctccgacca gcacccctgg ttccagcgcg
     8761 cgcgccgcgc ccaggccggc agcgcctggc gcgactacta cgtctggacc gacaaccccg
     8821 agaagtacaa ggacgcgcgc atcatcttta aggatttcga gacctcgaac tggtcgtggg
     8881 atcccgaggc cggcgcctac tactggcacc gcttctacca ccaccagccc gatctcaact
     8941 tcgacaaccc gcgcgtccac cgcgccatgt tctccacgct cgacttctgg ctgcgtatgg
     9001 gcatagacgg gctgcgcctc gacgccatcc cctacctcta cgagcgtgag ggcaccaact
     9061 gcgagaacct gcccgagaca cacgagttcc tgaaactctt gcggcggcac gtcgacgccg
     9121 agttcgacgg gcgcatgctg ctggccgagg ccaaccagtg gccggaggac gcggtcgcct
     9181 acttcggcga cggcgacgag tgccacatgt cgttccactt cccgctgatg ccgcgcatgt
     9241 tcatggcgct gcgcatggag gatcgctacc cgatcgtcga tatcatggcc caaacgccgg
     9301 aggtcccgcc gacctcccag tgggcgctct tcctgcgcaa ccacgacgag ctgacgctgg
     9361 agatggtcac cgacgaagag cgcgactaca tgtatcgcgt ctacgcccac gacccgcgca
     9421 tgcgcatcaa ccttggtatc cgccggcggc tggcgccgct cctgggcaac gaccgacgac
     9481 gcatcgagct gctcaacggc ttgctcttct cgatgcccgg caccccggtg atctactacg
     9541 gcgacgagat cggcatgggc gacaacatct acctgggcga ccgcaacggc gtacgtacgc
     9601 caatgcagtg gagcgccgac cgcaatgccg gcttctccga ggcgccgcgc cagcgcctct
     9661 acctgccggt gatcgtcgat gccgagtacc actacgaggc ggtcaacgtc gaagcccagc
     9721 aggccaaccc acactcgctc ctctggtgga tgaagcgctt gatcgcgctg cgtaagcagt
     9781 accgcgcctt cggccgcggc tcgctggagt tcctgcaccc ggacaaccgc cgcattctgg
     9841 cctacattcg ccagttccag gacgagcaga tcctggtcgt ggccaacctg tcgcgcttcg
     9901 tccaggcggt cgaattggac ctctccgcct accaggggct ggtgccggtc gagctgttcg
     9961 gacgcaacga attccctcag atcggcgagc agccgtactt cttgacgctc ggcccccact
    10021 ccttcttctg gttcaacctg cagccggccc gcgtcgaggt cagccagcgc gcgcccggat
    10081 tggagagctt gccggtcttg accgtcagcg gccgctggga gcatctgctg gagggcaagg
    10141 ggcgccgcga gctggcgcgc gtgctccccg gctacctggt cgatcgccgc tggttcggtg
    10201 gcaaggcgcg caagatcaag agtgtctccg tcaccgactc catgccgttc ccgttcgacg
    10261 agacacttgc ctttatcaca ctcgtgcagg tggattatgt cgagggggac agccaaacct
    10321 acctgttgcc gatcacctat gccccgccgg actacgcggt cgaactggag gcgctggcgc
    10381 cgcaggccct gatcgcacgg gtgcggagtc gcacccacaa cgtcgacggc gtcctcttcg
    10441 acgcgaccta cgatgccgcc ttcgacagcg ccctgctggc cgccgtgcgc ggccgccggc
    10501 ggctgcgcgg cgagggagag gtccgcgcgc tgccgctccc cgcgctgcgc gatctgctgg
    10561 tgctgagcga gggcgagctt tgcccggcgc cactgcgcgc cgagcagagc aactcgtcga
    10621 tctgctacga cgagcgcctg atcctcaagc tcttccggcg cgtcgagatc ggccccaacc
    10681 ccgacctcga agtgagccag ttcctgacca gccacggctt cgagcacaca cctgccgtcg
    10741 ccggctcgat cgaatacctc tcgccggacg gcaagtcggc ggcgctggcg atgctgcagg
    10801 gctacgtgcc gaaccagggg gatgcctggg agcacaccct gggtgtgctg gggcgcttct
    10861 tcgaacgcgc gatcaccgag ccgatcccgg atattccacc ctcgctggag gctgagcgtc
    10921 tcatcctgtt ggcaggccag gaactgccgg tcatcgcaca cgatctgacc aatggcttcc
    10981 tgggcgatgc cgagctcctg ggcgagcgca ccggggagat gcaccagacg ctcgcacgcg
    11041 ggcagaacgc cgccttcgcg ccggagccga tcaacagctt tagccagcgt tcgatctacc
    11101 agagcatgcg cgcgcaagcg ggtctggtgc tgccggcgct gcgcaagcag ttggggcgca
    11161 tgccggccga gctacgcgac gacgccgagg cggtactcga ggccgagggc cgcatccaga
    11221 gcgccttccg ccgcgttgtc tcgaccaaga tcgaggccag ccgcatccgc atccacggcg
    11281 attatcacct ggggcaagtg ctctacaccg ggcgtgattt ctcgatcatc gacttcgaag
    11341 gcgagccggc ccgcccgctc agcgagcgcc ggcacaagcg ctcgccgctg atcgacgtgg
    11401 ccgggatgct gcgctccttc cactatgcca cctacttcgg gctgcggcag gagcagggga
    11461 gccgggtgcg gccagaggac gtggccacgc tggaacgctg ggccggctgg tggaccgcct
    11521 gggtcagttc gcgcttcctg agcggctacc tgcgcgtcac cgccggcgca tcattcgtgc
    11581 ccgccgagcc ggcggccttc aacgccctgc tcgacgcctt cctgatcaac aaggcgatct
    11641 acgaggtggg ttacgagctg aacaaccgtc ccgagtgggt aagcatcccg ctcaagggcc
    11701 tgctgcgcct gatcgaggat tgatggcgcg gttcctgccc gcgaccagag aggtgaccgg
    11761 ctcggaacga gcgcgacctg cacgcgactc aatggaggga gaacgccctc gatggcgacg
    11821 acgctttccc ggttgacccc taacgacctg tacctcttca acgagggcac gcacatccgg
    11881 ctctacgaca agctgggcgc ccatccgacc gtggtcgacg gagtggccgg cgtcgacttc
    11941 gccgtctggg caccgaacgc gcgcggcgtc tcggtggtcg gcagcttcaa tggctggaat
    12001 ccggagcacg actggctcga gtcgctcggc aacgcgggca tctgggaagg cttcatcccc
    12061 ggcgtgggcc tgggcgagct atacaagttc cacatcgtcg ggccgaacgg ccagacgatc
    12121 gagaaggccg atccgttcgc cttccaggcc gagctgccgc cagccaccgc ctcggtcgtc
    12181 tgcgggctga actacgactg gagcgacgcc gagtggatgg ccggacgcgg ccggcgcctc
    12241 gcggccgacg cgcccgtcgc gatctacgag gtgcatatcg gctcctggat gcgcgtgccg
    12301 gaggagggca accgctctct cagctaccgc gagctggcac cgaagctggc cgagtacgtc
    12361 acccggctcg gcttcaccca cgtcgagttc ttgccgatca tggagcaccc cttcttcggt
    12421 tcctggggct accagatcac cggcttcttc gcgcccgaca gccgctacgg caccccgcag
    12481 gacttcatgt acctgatcga ctacctgcac cagcacggca tcggcgtcat cctcgattgg
    12541 gtgccctcac acttcccggt cgatgtccac gggctggcca gcttcgacgg cacgcatctc
    12601 ttcgagcacg ccaacccgat gcagggcttc caccccgact ggaacagcct gatcttcaac
    12661 tacgggcgcc acgaggtgca ggcgtttctg ctaagcagca tcatcttctg gctcgaccgc
    12721 taccacgtcg acggcatccg cgtcgacgcg gtcgcctcga tgctctacct cgactactcg
    12781 cgccagcccg gcgagtggat ccccaacaag tacggcgggc gcgagaacct ggaggcgatc
    12841 gccttcctgc gcacgatgaa cgaggtggtc taccgcgagt tcccggacgt gcagaccatc
    12901 gccgaggagt cgaccgcctg gccgatggtc tcgcgcccga cctatgtcgg cgggctcggc
    12961 ttcggcatga agtgggacat gggctggatg catgacacgc tcagctactt cagccaggat
    13021 ccgatccacc gcagctatca ccacaaccgg ctcaccttcc ggatgatcta cgccttcaac
    13081 gagaacttcg tgctgccgct ctcccacgac gaggtcgtcc acggcaaggg ctcattgatt
    13141 ggtaagctgc cgggtgacga ctggcagaag tttgccaacc tgcgcctcct cttcgcctac
    13201 atgtgggcgc agccgggcaa gaagctcctc ttcatgggcg acgagttcgg ccagtggagc
    13261 gagtggaacc acgatgggca gctgcaatgg gatctgcttc aatacccggc gcaccaggga
    13321 atcgagcgct ggctggccga tctcaaccgc gcctatcacg tcgagccggc gctgcatcgc
    13381 tacgacttcg actgggccgg cttccagtgg ctcgacgcga acgacgcacc caacagtgtg
    13441 ctcagcttct tccggctgag cgacaagccg gaggatcggc tcctctgcgt cttcaacatg
    13501 accccggtgc cgcgctacca ctaccgcgtc ggcgtgccgc tgctgggccg ctggcgggag
    13561 ttgctcaaca gtgacgccga gttctacggg ggtagtggcg tgggcaactt cggcggcgtc
    13621 gacacgaccc cgattccctc ccacggccag ccgcgctcga tcctcttgac cctgccgccg
    13681 ctggccgcac tcttcttcaa gcatgttccc gccgagccgc cctgggaggc ggccacagcg
    13741 tcgccagacg acgcgtgaga cccgagcacg atgcgcgact cgcacgtgcc gcgcgcgacc
    13801 taccgcgtgc aactcacacc ggagttcggc ttcgccgcgg cgcgtcgcct cgtgccctac
    13861 ctgcacgccc tcggcgtcgg cgatctctac tgctcgccga tcctcagcgc acgctccggc
    13921 agccggcacg gctacgacgt caccaacccc cacgagatca accccgaact gggcggccgc
    13981 atagagttcg atgcgcttac cgccgcgctc catgagcacg gcatgggcct cctgctcgac
    14041 atcgtgccca accacatggc cgccagcgtg gagaactcct ggtgggccga cgtgctggaa
    14101 cgcggcattg cctccccgta cgcgggctac ttcgacatcg agtgggactc ggcgctggcg
    14161 aacggagtgc tccagaaccg cgtcttactc ccgatcctcg gcgcgcccta cggccagacg
    14221 ctggagaacg gcgagctgcg gctggcgttg gagggcgacc acatcgttct gcgctacgcc
    14281 gaaacagcgc tgccgctcag cctggcgtca taccgccaca tcctgtgctg gcgcctccac
    14341 gccctcgacg cctcactggc cggcgagccg gagttagcac gcgcctatca cgcattgttg
    14401 gaacaattgg gccggctggc ggctgaagag gagaccgacc cgaccgcgcg ccaccatcag
    14461 cgccgtgcgc tccggccaga gctgcaccgg ctcctgggtt tccccgcgat ccgcgcgcac
    14521 ctcgaccatg cgctggcggc gatcaacggc agaccgggcc ggccgcgcag ttttgacctg
    14581 ctcgatcgca tcatcggcga gcaggcttac cggctggctt tctgggagct ggcgcgcgag
    14641 cgcatcaact accgccgctt cttcgacgtc aacgacctgg tcgccatccg ggtcgaggag
    14701 ccggacgtgt tcgtggccac ccaccaactg ttcctggagc tggcgagcca gcgacaggtc
    14761 agcggactac gcgtcgacca catcgacggg ctgcgcgatc cgctcggcta cctgcggcga
    14821 ctgcggcagg cgctggccga ggtgggcgcg gacgactgct acctggtggt cgagaagatc
    14881 ctctcgccgg gcgaatcgct gccagccggc tggcccgtcg ccggtacgac cggctacgac
    14941 tttctcaacg agttgggcgg gctctttgtc gatgacgcgg gcgacgccgc gctcgacacg
    15001 atctaccgcc gctggagcgg gctggtggtc gacttccccg agctggccta ccagcagaag
    15061 cgccgggtgc tcgacgacct cttcgccggc ggcgtcaacg agctcagcct ccggctcgac
    15121 cggatcgcgg agtcggatcg tcacgggcgc gacctgccgc tggcctcgct gcgacaggcg
    15181 ctgttcgagc taaccgcctc tctgaccgtc tatcgcacct acatccgcga cttcgccatc
    15241 gctcccgacg atcggcgctg gatcgagcgg gcgctcgccg acgcggcccg ccggcggccc
    15301 gagctcagcc acgcgctgac cttcctgcgc cggatcctcc tgttggagtt ccccgccaat
    15361 ctgccggccg aggaacggtc ggaccggctc gatttcgtat tgagctggca gcagttcacc
    15421 gggccggtga t
//
LOCUS       DASUBI010000096         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_364648, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000096 DASUBI010000000
VERSION     DASUBI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364648"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFU72_01845"
     CDS             <1..460
                     /locus_tag="VFU72_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422255.1"
                     /translation="RSGAGLRRDIVEVLGKLGIARERVDQPLEQLSRGMQQKVAIARA
                     FLTSPVLLLLDEPTTGLDPRSKLDVQHFVYQLRDEHDATILICSHDMDEAERLCDRIA
                     ILDQGRIVALDTVEGLKRRVAGTLGQAGITLEQVFIELTGRPLEAAEEVA"
     gene            499..1347
                     /locus_tag="VFU72_01850"
     CDS             499..1347
                     /locus_tag="VFU72_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422256.1"
                     /translation="MEFVETKRGGGGGSRSRFWREMRASYAFVERNFFLTKRYWGWEV
                     AFLIFNVTSSLSVMYIGKAQGAGQEQLILYLGIGTVVWAYLRSVFGNISEMIVWERWE
                     GTIEYTMMAPISRLTHMLGVALFAIIYGILRTALLLGALALFFEIDLSQANAWGALLI
                     ILVGSLSFVGVGIMAAVLPLLFTERGEEMTFIISSLLLLVSGVYYPISVLPGWMQAAA
                     RISPATYVLEGMRATILDGRPAGALGHYLLPMAVIGLITIPLGMLVFTTVERYAKRTG
                     RLKRSG"
     gene            1525..2070
                     /locus_tag="VFU72_01855"
     CDS             1525..2070
                     /locus_tag="VFU72_01855"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5422257.1"
                     /translation="MSMSHLRAVYLTGEQVYIRALTKEDAKAAVAWFDGPFPINEAGA
                     EEQLKELHSGNQWLGQKSIYAIARRSDDHVVAGVTVKIWRGTQADLKFHTAPWLDPGE
                     ADALRAEALALIVPWLRDEREVMAVQANIPADQPASLAAAEASGMTVAARLRRFIARP
                     GQRVDLLLCEALNPLWEARNA"
     gene            2063..>2292
                     /locus_tag="VFU72_01860"
     CDS             2063..>2292
                     /locus_tag="VFU72_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422258.1"
                     /translation="MRNAIMIGERVYLRPLEVSDADELARHYAVETETFFDDGRVPFS
                     PLAFERWIGDLYKQRPPREVQFAVCLKEDDRLL"
BASE COUNT          367 a          778 c          765 g          382 t
ORIGIN      
        1 ccgcagcggc gcgggactcc ggcgcgacat cgtcgaggtg ctgggcaagc tggggatcgc
       61 ccgcgagcgc gtcgatcagc cgctggagca gctctcgcgc ggcatgcagc agaaggtcgc
      121 catcgcgcgc gccttcctga cctcgccggt gctgctgctg ctcgacgagc cgaccaccgg
      181 cctcgacccg cgctccaagc tcgacgtgca gcacttcgtc tatcagttgc gcgatgagca
      241 cgacgccacc atcctgatct gctcgcacga catggacgag gccgagcggc tctgcgaccg
      301 catcgccatc ctcgaccagg gccggatcgt ggcgctcgat acggtcgagg ggctgaagcg
      361 ccgggtagcc ggcacgctgg gacaagccgg gatcacgctg gagcaggtct tcattgaatt
      421 gaccgggcgc ccgctggagg cggccgaaga ggtcgcctga gccggcgcgg gcgtacggat
      481 tgaggggacg gacgacgggt ggaattcgtc gaaacgaaac gtgggggcgg aggcggctcg
      541 cggtctcgct tctggcgcga gatgcgcgcc tcctacgcct tcgtcgagcg caacttcttt
      601 ctgacgaagc gttactgggg ctgggaggtc gccttcctga tcttcaacgt cacctccagc
      661 ctgagcgtga tgtacatcgg caaggcgcag ggcgccgggc aggagcagtt gatcctctat
      721 ctcgggatcg gcaccgtggt ctgggcctac ctgcgctccg tcttcggcaa catcagcgag
      781 atgatcgtct gggagcgctg ggagggcacc atcgagtaca ccatgatggc gccgatctcc
      841 cgcctgacgc acatgctagg ggtggcgctc ttcgccatca tctacggcat cctgcgcacg
      901 gcgctcctgc tcggcgcgct ggcgctcttc ttcgagatcg atctcagcca ggccaacgcc
      961 tggggcgcgc tgctgatcat cctggtcggc agcctgagct tcgtcggggt cgggatcatg
     1021 gccgcggtgc tgccgctcct cttcaccgag cgcggtgagg agatgacctt tatcatctcg
     1081 tcgctcctgc tgctggtcag cggggtctac tacccgatct cggtgctgcc gggctggatg
     1141 caggccgccg cgcgtatctc gcccgcgacc tacgttttgg aggggatgcg cgccaccatc
     1201 ctggatggcc ggccggccgg cgcgctcggt cactacctgc tgccgatggc cgtgatcggc
     1261 ctgatcacca tcccgctcgg catgctggtc ttcaccacgg tcgagcgcta cgccaagcgc
     1321 accggccgcc tgaagcgcag tggctagcgg ccctcatccc ccgccccttc tcccaaaact
     1381 tgggagaagg ggagctcgcc gcaggtacag agcgatgttc ccccaacttc ctcccctctc
     1441 ccagcattgg ctcgaacgga gccgtccgga gaggggctgg gggtgaggga ccataggcaa
     1501 tcaaggtacg aaaggagcga caccatgagc atgagtcatc tccgcgcagt ctacctgacc
     1561 ggcgagcagg tctacattcg ggcgctgacc aaggaggacg ccaaagctgc cgtcgcctgg
     1621 ttcgacggcc ccttcccgat caacgaggcc ggcgccgagg agcagctcaa ggagctgcac
     1681 agcgggaacc agtggctcgg ccagaagagc atctacgcca tcgcccgccg cagcgacgac
     1741 cacgtggtcg ctggggtgac cgtcaagatc tggcgcggca cacaggccga cctgaagttc
     1801 cacaccgcgc cctggctcga ccccggcgag gccgacgcgc tgcgggccga ggcgctggcg
     1861 ctcatcgtcc cctggctgcg cgacgagcgc gaggtgatgg cggtccaggc caatatcccg
     1921 gctgaccaac ccgctagctt ggcggccgcg gaggcgtccg gcatgaccgt ggccgcacgc
     1981 ctgcgccgct tcatcgcccg cccggggcag cgggtcgatc tcttgttgtg cgaggcgctc
     2041 aatcctctat gggaggcacg gaatgcgtaa cgcgatcatg atcggcgagc gggtctacct
     2101 gcgcccgctc gaagtcagcg acgccgatga gctggcccgg cactacgcgg tcgaaaccga
     2161 gaccttcttc gacgacggcc gcgttccctt cagcccgctc gccttcgaga ggtggatcgg
     2221 tgatctctac aagcagcgcc cgccgcgcga ggtccagttc gccgtctgcc tcaaggagga
     2281 cgaccggctg ct
//
LOCUS       DASUBI010000097         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_870477, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000097 DASUBI010000000
VERSION     DASUBI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870477"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..635
                     /locus_tag="VFU72_01865"
     CDS             102..635
                     /locus_tag="VFU72_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422259.1"
                     /translation="MAQLVMQRPHLRGAPAARPLPTGYPLRQAGAADEPALAETLTAA
                     FGETWTPERVRRELTGAPEVRAVYVIDCRGYVVATASSRVPLAHFPGAGCVHWVATHP
                     DHTRRGLGQVLLIRLLRDVAERGEAQAVLQTDDFRLPAIRAYLQCGFVPIYAVRDEDH
                     RERWSAIFQGMFQRGVK"
     gene            762..2855
                     /locus_tag="VFU72_01870"
     CDS             762..2855
                     /locus_tag="VFU72_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422260.1"
                     /translation="MRQQPVLTRRRLLQGLAALGGGALVAYYVERQPTTDAPADAATP
                     GTPPPATPASQAREAPALTQRVQQGDLPPLAARLPAQPLVLSSIDQSGAYGGEVHEPL
                     LSGDRTALERLLGNELLVRWRPDTRGFTADETVPNVAERITTSADGTTSTIVLRAGIR
                     WSDGEPLTTEDLRFWYEDVLGNADLRLAAPLDWLWPGGQAARVEVVDAQTLVLHFAAP
                     NGLFIPWLATPDGRDFVTHPAHYLKQFHQHYNPQVQALAAQHHTATWADLFRQQADTW
                     YNPDKPTLNAWLLATPLNTTGLSITAERNPYYWKVDPSGKQLPYLDRIVFSLLTPSGL
                     LTNVLAGNFDYEERYTTSQQNKPLFVDNEHKGQFHLFDTLVSRHNTLMLVLNLTHPNA
                     AKRQLFQSLDFRSGLSYAINRQQIIDTVFVGQGEPWQGAPRPESPFYHERLAKQYTEY
                     DVTRANAALDKIAPQRDAADRRLGPDGQPIQIQIVYGGGQTFTDCLQLIKRDAEAVGI
                     TIDIRPVTGGNATQIVAHNEHDATVAIGEHGLEVLLAPEWYLPTNGMTAPYAPPWARW
                     FQSPDAPGASEPPAIAQRQMALYRQLQATPNVAQQHALMRQILDLAADAFYAIGISLH
                     PPGYGMVKNTLHNVPAAMLSSPLFEEPAPLNTCLFYRDAEARSGETAAVLRSGKTSGS
                     DVAPAPRPGSSQARA"
BASE COUNT          526 a         1000 c          909 g          447 t
ORIGIN      
        1 agccccctct cccgtgatac gggagagggg gagccgagca acgcgaggcg ggggtgaggg
       61 ctgtcccttg atgtgatgta tggaaaggag caccggtaag catggcgcaa cttgtgatgc
      121 agcgcccaca cctgcgcggc gcgccggcgg cgcgaccgct gcccaccgga tacccgctgc
      181 gccaggcggg cgcggctgac gaaccggcac tggcggagac gctcacggcc gcgttcggcg
      241 agacctggac gccggagcgt gtgcgccgtg aactgaccgg cgcgcccgaa gtccgcgccg
      301 tctacgtcat tgactgtagg ggatatgtcg tggcgacggc gtcgagccgc gtcccgctgg
      361 cgcactttcc cggcgcgggc tgcgtccact gggtcgcgac gcatcccgac cacacacggc
      421 gcggcctggg gcaggtgctg ctgatccgcc tgctgcgcga cgtcgccgag cgcggcgagg
      481 cccaggccgt tcttcagacc gacgacttcc gcctgcctgc catccgcgct tacctccaat
      541 gcggcttcgt tcccatctac gccgtgcgcg acgaggatca ccgggaacgc tggtcggcta
      601 ttttccaggg gatgtttcaa cgtggcgtga agtagaccgc gacaggcagg cggcggcgta
      661 acaaaatcgg tcctgttgcg atagaacgag tgaggccagc gacacagcta catcaagctg
      721 gctggccgtg ccggcctgcg tgttacggtt cacgaggatg tatgcggcaa cagccagtac
      781 tgacgcgccg ccgtctgctc cagggactgg ccgcgctagg cggcggcgca ctcgtcgcct
      841 attacgtcga gcgccaaccg acgaccgatg cgccagcaga cgcggcgaca cccggcaccc
      901 cgccgccggc cacaccagcc agtcaggccc gcgaagcgcc cgcgctcacg cagcgggtcc
      961 agcaagggga tctcccgccg ctcgccgccc ggctgccggc acagccactg gtgctcagct
     1021 caattgatca gagcggcgcc tatggcggag aggtacacga gccgctgctc tccggcgacc
     1081 gcacggcgct ggaacggctg ctgggcaatg aactactcgt ccgctggcgg cccgacacca
     1141 gaggcttcac cgcagacgag accgtgccga acgtggcgga gcgcatcacc accagcgccg
     1201 atggcacgac ctctaccatc gtcctgcgcg ccggtatccg ctggtcggac ggtgagccgc
     1261 tgaccacgga ggatttgcgc ttctggtatg aggatgtcct gggcaacgcc gacctccgcc
     1321 tggccgcgcc gctggactgg ctctggccgg gcggccaagc cgcgcgggtt gaggtggtgg
     1381 atgcccagac actcgtcttg cacttcgccg cgccgaacgg cctcttcatc ccctggctcg
     1441 ccacgccgga cggccgcgac ttcgtgaccc acccggcgca ttacctcaag cagttccacc
     1501 aacactacaa cccgcaggtg caggcactcg cggcccagca ccacaccgcc acctgggcgg
     1561 atctcttccg tcagcaggcc gatacctggt acaaccctga caagccgacg ctcaacgcct
     1621 ggctgctggc gacaccgctc aacacgaccg gcctgagcat caccgccgag cggaatccct
     1681 attactggaa ggtggaccct tccgggaagc agttgcccta tctcgatcgc atcgtcttct
     1741 cactgctgac tccgagtggc ctcctgacga acgtgctagc cggcaacttc gactacgagg
     1801 aacgctatac gacgagccag cagaacaagc cgctctttgt ggacaatgag cataagggcc
     1861 agtttcacct cttcgatacg ctggtctctc gccacaacac gctgatgctc gtgctgaacc
     1921 tgacccatcc gaatgcggcg aagcgccagc tcttccagag tctggacttc cgcagtgggc
     1981 tgtcctacgc gatcaaccgg cagcagatca ttgacaccgt cttcgtgggg cagggcgaac
     2041 cgtggcaggg cgcgccgcgt ccggagtcac cgttctatca cgagcggctg gcgaaacaat
     2101 acaccgagta cgacgtcacg cgggcgaatg ccgccctgga caagattgcg ccgcagcgcg
     2161 acgccgccga ccggcggctc ggccctgatg ggcagccaat tcagatccag attgtctacg
     2221 gcggcgggca gaccttcacc gactgcctgc aactgatcaa gcgcgatgcc gaggccgtcg
     2281 gcatcacgat tgacattcgc ccggtgacag gcggcaacgc gacccagatt gtcgcgcata
     2341 acgaacatga cgccacagtc gcgatcggcg aacatggcct ggaggttttg ctggcgccgg
     2401 aatggtatct acccaccaac ggtatgaccg cgccatatgc gccgccgtgg gcgagatggt
     2461 tccagtcgcc cgacgcgccg ggcgcgagtg agccgcccgc gatcgcccag cggcaaatgg
     2521 cgctctaccg gcaattgcag gccacgccga acgtcgccca acagcacgcg ctgatgcggc
     2581 aaatcctcga cctggccgcc gatgccttct acgctatcgg catcagcctg cacccgcccg
     2641 gctatggcat ggtcaagaac accctgcaca atgtgccggc ggcgatgctg agttcaccgc
     2701 tgttcgagga accagccccg ctcaatacct gcctctttta tcgagacgca gaggcgcgga
     2761 gcggcgaaac agcagcagtg ttgcgaagcg gcaagactag cggtagcgat gtcgccccag
     2821 cgcctcgccc cggcagtagc caggcgcggg catgatagta gaacgcgctg ccagtcgttt
     2881 cc
//
LOCUS       DASUBI010000098         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_324282, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000098 DASUBI010000000
VERSION     DASUBI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324282"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFU72_01875"
     CDS             complement(<1..500)
                     /locus_tag="VFU72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5422261.1"
                     /translation="MDPVVEARGLTKHFGDVEALSGLDLVAESGQVTALLGPNGAGKT
                     TFISAVATLLRPDGGDLWVAGIDIRSAPDRVRQVIGLAGQSASVEPAMTGRENLEFVG
                     RLFGLDRRAARSAAAAVLEQLDLTDAGDRLVRTYSGGMRRRLDLGASLVGSPRLLLLD
                     EPTTGL"
     gene            591..1478
                     /locus_tag="VFU72_01880"
     CDS             591..1478
                     /locus_tag="VFU72_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5422262.1"
                     /translation="MNENEIEWLAECFERHRPHLRAVAYRMLGSLAEADDAVQDAWLR
                     LSQSKADEIDNLGGWLTTVVARECLHMLRRRRLRREDALAAHLPDPIIVPDRDLDPEQ
                     QALLADSVGLALLVVLDSLTPAERLAFVLHDMFELPFDEIAPMVGRTPTAARQLASRA
                     RRRVKGAGIPAPDPDLTRQRAVVDAFFSASRAGDFDALIQVLDPDVRLRVDFGPSRPP
                     ALIRGVSAVAKLARAPRGADLHPALVNGMVGAVITLNGRPSSVLAFTVANDKIVEIDG
                     IGDPARVRRIAAAVLTGLA"
     gene            complement(1527..1745)
                     /locus_tag="VFU72_01885"
     CDS             complement(1527..1745)
                     /locus_tag="VFU72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5422263.1"
                     /translation="MRVLGAQPARVGGVWRASAGRAAPHHPGQGEIAEALFLSPRTVQ
                     NRVKHILAKLGVESRTGAATYAVRQGFV"
     gene            complement(1770..>2551)
                     /locus_tag="VFU72_01890"
     CDS             complement(1770..>2551)
                     /locus_tag="VFU72_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422264.1"
                     /translation="HFHAARALWSQMGSEEDLAGVDYHLGIVAYGRGNLPAAVDLLSR
                     ASATASAGGDRMLSAWCSWYLALVACDQKAPIEAAAALGEYHHEADPALSQSLHWAKH
                     YAAAAVLAAAMGENETAARLFGAMRAESAHDPLGWPERETIDRVAAVVRARLGEEADD
                     AAQQAGARLPAGEVVAEIDRLLDRAAAAPVPRVATYLPITPREQEVLRLVVDGKSNQQ
                     IADALYLSPRTVSTHITSILSKLHVDSRTAAATYAIRHQLA"
BASE COUNT          383 a          903 c          871 g          394 t
ORIGIN      
        1 tcgaggccgg tcgtcggctc gtcgagcaac agcagcctgg gacttccgac caggctggcg
       61 ccgagatcca gccgccgacg catacccccc gagtaggtgc gcaccaggcg gtctccggca
      121 tccgtgaggt ccagctgctc caggaccgcc gcggcagccg accgggctgc gcggcggtcc
      181 agaccgaaga gccggccaac gaactccaga ttctcacgac cagtcatggc cggctccaca
      241 gatgccgact ggcccgccag gccgatgacc tggcggacgc gatccggggc ggatctgatg
      301 tcgatgccgg caacccacag atcgccacca tcggggcgca gcagggtggc aaccgcgctg
      361 atgaaggtcg tcttgcccgc gccgttcggc cccagcaagg cagtgacctg tccggactcc
      421 gcgacaagat cgagaccact gagggcctcg acatcgccaa aatgcttggt gaggcctcgc
      481 gcctcgacaa ctgggtccat cgttcgttct ccttgcatgt cattgctggt cacaatcgtc
      541 gcgtcccatg gatcatctct atgagccgtg gcgacaggat ggtgtgactg atgaacgaga
      601 acgagatcga gtggttggcc gagtgcttcg agcggcaccg gccccacctc cgggcagtgg
      661 cctatcggat gctcggttcg ctggccgagg ccgacgacgc cgtgcaggat gcctggctcc
      721 gactgagcca atcaaaggcg gacgagatcg acaacctcgg cggatggctg acgaccgtcg
      781 tcgcccgaga atgcctgcac atgttgcgtc gccgtcgcct gcgtcgcgag gacgcgctcg
      841 ccgcgcacct gcccgacccg atcatcgtcc ccgaccggga tctcgacccg gagcagcagg
      901 cgctgctggc cgactcggtc ggtctggccc tgctcgtggt gctcgactcg ctgaccccgg
      961 ccgagcggct ggcgttcgtg ctccacgaca tgttcgagct gccgttcgac gaaatcgccc
     1021 ccatggtcgg gcgcaccccg accgctgccc ggcaactcgc cagccgcgcg cggcgccgcg
     1081 tgaaaggcgc cgggataccg gcgcccgacc ccgacctgac gcgtcagcgc gccgtcgtcg
     1141 acgccttctt ctcggcgtcg cgggccggcg actttgatgc gctcatccag gtcctcgacc
     1201 ccgacgtcag actgcgtgtc gatttcggcc ccagccggcc gccggcgctc attcgcggcg
     1261 tgtcggccgt agccaaactg gctcgcgcgc cacgtggtgc ggacctgcac cctgcgctgg
     1321 tcaatggcat ggtcggagcg gtgatcaccc tcaatgggcg gccctcctcc gtcctggcct
     1381 tcaccgtcgc caacgacaag atcgtagaga tcgacggcat cggcgacccc gcccgcgttc
     1441 gccggatcgc agccgccgtt ctcaccgggc tggcgtgagc aggacggtga acgcagcacg
     1501 tggccggcgg tggcgcggca tggcgatcag acgaatccct gccgcacggc gtaggtcgcc
     1561 gcgccggtgc gggattcgac accgagcttg gccaggatgt gctttacgcg gttctgcacg
     1621 gtgcgcgggc tgaggaagag cgcctcggcg atctcgcctt gtcctggatg atgaggcgca
     1681 gcacgtccgg cagaagcgcg ccagacaccg ccgacgcgtg ccggttgcgc gccgagaacg
     1741 cgcaaccggc atcgatcatc acccggtgat caggcgagct ggtggcggat ggcataggtc
     1801 gccgcggccg tgcgggagtc gacgtgcagc ttgctgagga tgctggtgat gtgcgtggag
     1861 accgtgcgcg ggctgagata gagcgcgtcg gcaatctgct ggttgctctt cccatccacg
     1921 acgagacgca gcacctcctg ctcacgcggg gtgatcggaa ggtaggtggc gacgcgcggg
     1981 acgggcgcgg ccgccgcgcg gtcgagcagg cgatcgatct cggccacgac ctcaccagcc
     2041 ggcagacggg cgccggcttg ctgcgccgcg tcatcggcct cctcgcccag ccgggcgcga
     2101 accacggccg cgacacggtc gatggtctcc cgctccggcc agccgagcgg gtcgtgcgcg
     2161 ctctccgcgc gcatcgcgcc gaagagccgg gcggcggttt cgttctcccc catcgcggca
     2221 gccagcacgg ccgccgcggc gtaatgtttg gcccagtgga ggctctggct cagggcgggg
     2281 tccgcctcgt ggtggtactc gcccaatgcc gcggccgcct cgatcggcgc cttctgatcg
     2341 cacgccacca gggccaggta ccagctacac cacgcggaga gcatgcggtc gccgcccgct
     2401 gacgccgttg cgctcgcgcg gctcagcaga tcgacggccg ccggcaggtt gccccgccca
     2461 taggccacga tcccgaggtg gtaatcgacg ccggcgaggt cctcttcgct gcccatctgg
     2521 ctccacagcg ctcgggccgc gtggaagtgt g
//
LOCUS       DASUBI010000099         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_596765, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000099 DASUBI010000000
VERSION     DASUBI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596765"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU72_01895"
     CDS             complement(<1..201)
                     /locus_tag="VFU72_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422265.1"
                     /translation="MPDWDASPHTSEQISETATRLHSVAIHLLRRLRREDDASGLTAP
                     RLSALSVVVFGGPLTLGALAAAE"
     gene            268..675
                     /locus_tag="VFU72_01900"
     CDS             268..675
                     /locus_tag="VFU72_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5422266.1"
                     /translation="MSAASLASPTFGLSRLGQIALNAHDLDRAVAFYRDRLGLTFLFR
                     APNLAFFDCADVRLMLALPERPELDHPGSILYFSVDDLNAAYETLNQRGVTFTDRPHL
                     IARMPDHELWMAFFVDTEGNTLGLMSEVRTQPQ"
     gene            complement(1011..1517)
                     /locus_tag="VFU72_01905"
     CDS             complement(1011..1517)
                     /locus_tag="VFU72_01905"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422267.1"
                     /translation="MRATDVLAILDCLAAAGAAAWLDGGWGVDALAGEQTREHDDLDL
                     VIAIEQLPAALATLNTLGFTVATDELPTRLVARDPADRRIDFHPVTWDSGGGGHQALP
                     DGTTFRYPPEGFTGQGVVAGRPVACLTAEVQARCHLGYSPDETDYHDMRLLRDRCGVA
                     LPPPYDGV"
     gene            complement(1580..2029)
                     /locus_tag="VFU72_01910"
     CDS             complement(1580..2029)
                     /locus_tag="VFU72_01910"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5422268.1"
                     /translation="MGILTADMQRLVRAQRLGYVATVCPDGTPNLSPKGTVTVWDDDH
                     LVFADIRSPRTVANLRHNPVLEINVVDPFSRLGYRFKGTATIHESGDLFDRIVACYRE
                     QGLESTVRRAVLVEVTRALPLRSPAYDQPVTEAEVRARWERIYLGER"
     gene            complement(2166..>3024)
                     /locus_tag="VFU72_01915"
     CDS             complement(2166..>3024)
                     /locus_tag="VFU72_01915"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5422269.1"
                     /translation="AAKPFQALPIITSGAAEAFDLTSEELAICASSHSGEPVHLAVVG
                     GLLAKLGLDETALQCGAVPPIDREVAAQVETGALAPSAFYCDCSGKHAGMLAVCRHRG
                     EPLESYRDPAHPHQQEIARVMSDFLGLPERAIPRGIDGCGVPTFAAPLARIAAAWAGL
                     AAPRPGPYRAATTRVLDAMAAHPYLVGGRGRVDTDLMECTGGAVVAKAGAEGVLCLAL
                     RERGWGVAIKVEDGSFRGMAAIAAQTLRQLHLLDDTAIACLLERQPLTVRNNTGVVVG
                     EVRPAFSLR"
BASE COUNT          489 a         1096 c          964 g          475 t
ORIGIN      
        1 ttcggccgcc gcgagcgcgc caagcgtcag cgggccgccg aaaactacga ccgagagcgc
       61 cgacagccgg ggcgcggtca gaccactcgc gtcatcttcc cggcgcagcc gccgcaagag
      121 atgaatcgcg accgaatgaa ggcgtgtggc cgtctcgctg atctgctcgc tggtgtgcgg
      181 gcttgcatcc cagtcaggca tcccatatcc tattcgttag cgatactaag tatttgtacg
      241 ttcacatccg tgaggaggaa cctcgccatg agcgccgcat cactggcttc gccaaccttt
      301 ggtctgtccc gcctgggcca gatcgcgctg aacgcgcacg atctcgaccg tgccgtcgcc
      361 ttctaccgcg accggctcgg tctgaccttc ctctttcgcg cgccgaacct ggccttcttc
      421 gattgcgccg acgtgcgcct catgctcgcg ctccccgagc gtccggaact cgaccatccc
      481 ggctcaattc tctatttcag cgttgacgac ctcaacgccg cttacgaaac cctcaaccag
      541 cgcggcgtca ccttcaccga ccggccccac ctgatcgcgc gtatgccgga tcacgaactc
      601 tggatggcct tcttcgtgga caccgaaggc aacacactcg gcctcatgag cgaggtccgc
      661 acgcagcccc aataatgcca tagtcgggca gacgagcagt cgggcaggcg ggcagtgggt
      721 tcaggccgac accaagcctg gctatgggcg cggcgacgcc gaggtgcgcg ccgaatcgca
      781 gtccggcgcg aagggcctcc gaccacaaag cccctacggg actagggctg gtcaactact
      841 acgccaacag tcagccccgg tatggcaacg aacccagtcc gcgcaggcgg acttcgtggc
      901 ccccgcaggg gccttcagcc cgcggtttca accgcccggg caacctcagc agcaccgcgc
      961 ccgcagacca gccaccgtgc cggcataaac ccactgctcg accacccgac ttacaccccg
     1021 tcatacggcg gaggcagcgc cacgccgcag cggtcgcgga gcaggcgcat atcgtgatag
     1081 tcggtctcgt caggcgagta gccgagatgg cagcgcgcct gcacctcggc ggtcaggcag
     1141 gccaccggcc gcccagcgac cacaccctgg ccggtgaagc cttccggcgg gtagcggaag
     1201 gtggtgccat ccggcagcgc ctggtggccg cctccgccgc tgtcccacgt caccgggtgg
     1261 aagtcaatgc gccggtctgc ggggtcacgc gcgacgagcc gcgtcggcag ttcgtcggtg
     1321 gcgacggtga agcccagcgt gttgagcgtc gccagcgccg ccggtagctg ttctatcgcg
     1381 atcaccaggt ccaggtcatc gtgttcgcgc gtctgctcgc cggccagcgc gtccacgccc
     1441 cagccgccgt cgagccaggc ggcggcgcca gcggcggcca ggcagtcgag gatcgccagc
     1501 acatcggtgg cgcgcatcat ggttctgtgt ggtggtctcc atcagcgccg gcaactcccg
     1561 gcctgctacc cgccgggctt catcgttccc ccaggtaaat ccgctcccag cgggcgcgca
     1621 cttccgcttc ggtcaccggt tggtcatacg ccggggagcg caacggcagg gcgcgggtga
     1681 cctcaaccag cacggcgcgc cgtaccgtgc tctccaagcc ttgctcgcgg tagcaggcga
     1741 caatgcggtc gaaaagatcg ccgctttcat ggatcgtcgc ggtgcctttg aagcgatacc
     1801 caaggcggct gaacgggtcc acgacgttga tctccagcac cgggttgtgg cgcaggttgg
     1861 cgacggtgcg cggcgagcga atgtcggcga agacgagatg atcgtcgtcc cagaccgtca
     1921 ccgtcccctt tggcgagaga ttcggtgtgc catcgggaca gacggtggcc acatacccca
     1981 ggcgctgggc gcgcaccagg cgctgcatat cggcggtcag gatacccatc agtcacgctc
     2041 ctatacgagt cgggcagccg ggcggtcggg cagtgggttc cggtcagcat ggtgcctgtc
     2101 ttggggctac ggtgctcatc caacgcactg cccgactgct cgactgcctg actcgcccgc
     2161 gctcgtcacc gtagcgaaaa ggccgggcgc acttccccga cgactacgcc ggtattgttc
     2221 cgcacagtca gcggctggcg ttccagcagg caggcgattg cggtgtcgtc gagcaggtgc
     2281 agctggcgca gcgtctgcgc ggcaatcgcc gccataccgc gaaaactgcc gtcctcgacc
     2341 ttgatcgcca cgccccagcc gcgctcgcgc agcgccaggc ataatacgcc ctccgcgccg
     2401 gctttcgcca cgaccgcgcc cccggtgcat tccatcaggt cagtgtccac ccgtcctcgt
     2461 cccccaacca gatacgggtg cgccgccatc gcgtcgagca cgcgcgtcgt cgccgcgcgg
     2521 tagggaccgg gacgcggcgc ggccagcccg gcccaggccg ccgcgatccg ggccagcggc
     2581 gcggcgaagg tcggcacgcc gcagccatca atgccgcgcg ggatggcgcg ttccggcagg
     2641 cccaggaagt cgctcatgac gcgcgcgatc tcctgctgat gtggatgcgc cggatcgcgg
     2701 taactttcca gcggctcgcc gcgatgccgg cacaccgcca gcatccccgc gtgcttgccc
     2761 gagcagtcgc agtagaacgc tgacggcgcc agcgcgcccg tctcaacctg cgcggccacc
     2821 tcccggtcaa tcggcggcac ggcgccgcat tgcagcgcgg tctcgtccag gccaagcttg
     2881 gcgagtaggc cgccgaccac cgccagatgg accggttccc cgctatgcga cgacgcgcag
     2941 atcgccagtt cttcgctggt caggtcgaac gcctccgccg cgccgctggt gatgatcggc
     3001 agcgcctgga acggcttggc cgcc
//
LOCUS       DASUBI010000100         5887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1087428, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000100 DASUBI010000000
VERSION     DASUBI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087428"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1136
                     /locus_tag="VFU72_01920"
     CDS             321..1136
                     /locus_tag="VFU72_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422270.1"
                     /translation="MAYQFNPPPNWPQPPVGWVPPPGWQPDPSWPPPPPGWQLWIEVP
                     PTPPGWSAPVGPVPASAAASFVSPAAVISAQSVEAPPSTPLADPGRTAPAGRFGGRRK
                     HAEAEAEQARGEVARLSGQVATLQAQLAAVTAERSAVAQELSRVRGLDADQLAHEIDR
                     MRAQLAEAQQQLERDRATADEEAARRKRELEQSLDQVRQHAEQEAVAANARRDTAKLE
                     AQQEETRLRELKAAIVRADETAMLQEVGIYVYRHVLADAVAYKSTLADLTDGI"
     gene            1215..2057
                     /locus_tag="VFU72_01925"
     CDS             1215..2057
                     /locus_tag="VFU72_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEU5422271.1"
                     /translation="MVSDFSKLMLRAYNAEADYAVRSMRPHRLQSLIERLDKSRTTIA
                     RLGSTMDIRITDQYHRLRVRELELTADFLAKQDEEKERRREVREAQRELQREIARERE
                     RLAKERAKYVGALARLRASGQTMETGGLAELEAKLAEVEAAVAAVDAREANIRAGYVY
                     VISNVGAFGDSVVKIGLTRRLEPMERINELGDASVPFRFDVHALIFSEDAVTLERKLH
                     QEFEPRRVNRVNLRREFFRATPAEVRDALARYNGQHLLEFHEEAEALEWRASHNQESV
                     SAAA"
     gene            complement(2104..2487)
                     /locus_tag="VFU72_01930"
     CDS             complement(2104..2487)
                     /locus_tag="VFU72_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5422272.1"
                     /translation="MSPNSETATTPTTTSIATSTASTSTASPTLEKAPAADQGTKSVD
                     SVALTVTLPANLHARPAGKLAQTAARFNSAIRLDCGAKSVNPKGVLAVMSLGATAGTS
                     VTLRAEGDDAEQAVQALADVLANAE"
     gene            complement(2471..2863)
                     /gene="dhaM"
                     /locus_tag="VFU72_01935"
     CDS             complement(2471..2863)
                     /gene="dhaM"
                     /locus_tag="VFU72_01935"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEU5422273.1"
                     /translation="MEQPVGIVLVSHSAQLAAGLRELLAQIGSDAVPVAAAGGAPGGG
                     IGTSYDLIHAAITEVDRGAGVLVLPDLGSSVLTTRTVLDDHPRPDVVLVDAPFVEGAV
                     AAAVTAAMGADLDAVAHAAKEARDVAKL"
     gene            2923..3699
                     /locus_tag="VFU72_01940"
     CDS             2923..3699
                     /locus_tag="VFU72_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU5422274.1"
                     /translation="MVQSVQRAVQILRELAAGGPRLGVTELADRVGVAKPTVHALLRT
                     LEGEGLVVQDAETGKYQLGPGLLMLGNAYLDTQELRARSLTWADALANRTGEAVWVAV
                     LTGDHVFVVHHAFRPEGAVQILEVGASIPWSTCALGKAIVAFLPPDHRKSLLAAELPA
                     LTGWSVTDSQAVAEQLDAVVKSGYALEDQESALGDAGIAAPVFDRSGGVAGAIGIVGP
                     IERLFADSARQDHVVAVREVARHLSRELGAGRGAMRGMRL"
     gene            4024..4824
                     /locus_tag="VFU72_01945"
     CDS             4024..4824
                     /locus_tag="VFU72_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEU5422275.1"
                     /translation="MDAHRPAGRGRVMPENTFPQKLLAELLGTAMLVFIGVGSVPATL
                     IIGGKAPFTMAELGMISFAFAMVVVAMIYAIGHISGCHINPAITLALAATGKLPWRDV
                     PGYIAAQVAGATLGAFAIVGVLGHKAVDVGLGIASYSAPVGAGRAFFAEAVGTFILAF
                     VVFGAFDGRAPGGFGGVAVGLAVFAIIIPVGPATSASINPARAIGPMLVGQLFNGTVH
                     WNQLPVYLAAEIIGAVLAGLTYVALNTKRAAVRSVSTPQPVPAEGVSA"
     gene            4821..5819
                     /gene="dhaK"
                     /locus_tag="VFU72_01950"
     CDS             4821..5819
                     /gene="dhaK"
                     /locus_tag="VFU72_01950"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU5422276.1"
                     /translation="MKKLINTPESVLADALAGIAAAHPDLAVDRDARIITRASGPNRG
                     KVALISGGGSGHEPLHGGFVGPGMLNAACPGEVFTSPVPDQMLAAARAADGGAGVVFI
                     VKNYTGDVMNFEMAADLAADDGISVATVLVDDDVAVKDSTWTAGRRGTGATVLVEKIA
                     GALAEAGGDLAAVAELGTRVNKASRSFAVALTACTTPAAGKPGFELGEDEMEVGVGIH
                     GEPGRSREKLRPAAEIVATALDAILADHELDAGDEVIVLVNGLGGTPLIELYVAFAEV
                     AAILAAQGVRIARSLVGNYVTSLDMAGVSLTVCKADEQMLALWDAPVNTPALRWGV"
BASE COUNT          947 a         1928 c         2043 g          969 t
ORIGIN      
        1 ggcgcggtct agcgagatgg cctcaacggc gatcggctca ggcgtgtgat cagggtaagt
       61 tgcatagatg tagagaggcc atgggcgtta cgcacatgga agaaaatcat ctggctgacc
      121 cgtgttcggc gcgattcagc cgattgggct tgtaacgaac cggtatgggt tgggtctccg
      181 ttgaggtgct actcggcgaa cgttgccatg cgcatatatg ctatctgata gccgcaccaa
      241 aggcgcgtat tagtcggcgt ttccactaat gcgtggcatg gttctgccag cggctagcgg
      301 acgcataggg gagggcgtta atggcatacc aattcaatcc gccgccgaac tggccgcaac
      361 cgccggtcgg ctgggtgcca ccgccgggct ggcagcctga cccgagctgg ccgccgccac
      421 cgccgggatg gcagctgtgg atcgaagttc ctcctacacc cccgggttgg tccgcgccgg
      481 ttggaccggt tccggcttca gccgctgcct ccttcgtttc acctgccgct gttatttcag
      541 cgcagtcggt cgaggctccg ccttcgaccc cgcttgctga tcccggccgc accgcgccgg
      601 ccgggcggtt tggtggccgc cgcaagcatg ccgaagcgga ggcagaacag gcgcgcggcg
      661 aggtcgcccg cctctccggc caagtcgcca cgcttcaagc tcagctcgcg gcggtgactg
      721 ccgagcgatc tgcagtcgcg caagaactct cccgcgtgcg cggactagat gctgatcagc
      781 ttgcgcacga gattgatcgg atgcgggcgc agttggctga ggcgcagcaa cagcttgaga
      841 gggaccgcgc gaccgcagac gaggaggcgg cgcgtcggaa gcgcgaactt gagcagagcc
      901 tggatcaagt gcggcagcac gccgagcagg aggcggtcgc cgcgaacgcg cgccgcgaca
      961 ctgccaagct cgaagcgcag caggaggaga ctcggctgcg ggagctgaaa gcagcgatcg
     1021 tacgtgcgga cgagaccgca atgctgcaag aggtcggtat ctacgtatac cggcacgtgc
     1081 ttgccgatgc ggtggcgtac aaatcaacgc tcgccgatct gaccgatggc atctagacca
     1141 cagcgcgcaa ggggcaagcc gtcgtcggca gcacgaactg gactgtgaac gggtcagcga
     1201 cgcaaggccg ccgaatggtc agtgacttct ccaagctgat gctgcgcgcc tataacgccg
     1261 aggctgacta cgcggtgcgc agtatgcgcc cgcatcgcct gcaatcgctg atcgagcggc
     1321 tagacaagtc acgcaccacg atcgcgcggc tcggctccac gatggacatc cgcatcaccg
     1381 accagtacca ccgactgcgg gtgcgcgaac tggagctgac cgctgacttc ctggccaagc
     1441 aggacgagga gaaggaacgg cgccgtgaag tgcgcgaggc gcagcgcgag ctgcagcgcg
     1501 agatcgccag ggagcgcgag cggctggcta aggagcgtgc gaaatacgtc ggtgcgctcg
     1561 ccaggctgcg cgcctccgga cagacgatgg aaaccggcgg gctggccgag ttggaagcca
     1621 aactggcgga agtcgaagcc gctgtggcag ccgtagacgc ccgcgaggcg aacatcaggg
     1681 ccgggtacgt gtacgtcatc tccaacgtcg gcgcgttcgg cgacagcgtg gtgaagatcg
     1741 ggctgacccg acgcttggag ccgatggagc ggatcaacga actcggcgat gcttcagtgc
     1801 cgttccgctt cgacgtccat gcactgatct tcagcgagga cgcggtgacg ctggaacgca
     1861 agctgcatca agagttcgaa ccccggcggg tcaaccgagt caatctgcgc cgcgagtttt
     1921 tccgagcgac gccggccgag gttcgcgacg cgctcgcgcg atacaacgga cagcacttgc
     1981 tggaattcca cgaagaggca gaggccttgg aatggcgcgc cagccacaac caagaatccg
     2041 tcagcgcggc ggcgtgaact gcgccgtgtt catccgattc atcctgtctg gttccggcgg
     2101 gcgttactcc gcgttggcga gaacgtcggc gagggcttgc accgcctgct cggcgtcatc
     2161 cccctcggct cgtagcgtca cgctcgtccc ggctgtggcg cccaggctca tgacggccag
     2221 cacacccttt gggttgaccg actttgcgcc gcagtccagc cggatggcgc tgttgaagcg
     2281 ggcggcggtc tgggcgagct ttccggccgg gcgggcgtgc aggttggcgg ggagcgtcac
     2341 ggtgagcgcg acgctgtcga ccgacttcgt tccctgatcg gctgccggcg ctttctcgag
     2401 agtcggtgac gcggttgacg tcgacgcggt cgacgtcgcg atcgacgtcg tagtcggtgt
     2461 tgtggctgtc tcagagtttg gcgacatcgc gggcctcctt ggctgcgtgg gctacggcgt
     2521 cgaggtcggc tcccatggcg gcagtcaccg ccgcggcgac ggcgccctcg acgaaggggg
     2581 cgtcgaccag caccacgtcg ggacgcggat ggtcgtccag gacggtgcgg gtggtgagca
     2641 ccgaactgcc gaggtcgggc agcaccagca cgcccgcacc ccggtccacc tcggtgatcg
     2701 ccgcgtggat caggtcgtag ctggtgccga tgccgccacc tggcgccccg ccggccgcgg
     2761 ccaccggcac tgcgtcggag ccgatctggg cgagcagttc gcgcaggccc gccgcgagtt
     2821 gggcgctgtg agatacgagt acgatgccga ctggttgttc catgaacggt gatactaaat
     2881 cccggaacgg tgttcagcaa tactgaaagc ggtgtcggtt cgatggtgca gtcggtgcag
     2941 cgcgcggtgc agatcctccg ggagctggcc gccgggggtc cccgcctggg cgtcaccgag
     3001 ttggccgacc gggtcggagt ggcaaagccg accgtgcatg ccctgctgcg cacgctggag
     3061 ggcgagggtc tggtcgtgca ggacgccgag accggcaagt accagctcgg cccgggattg
     3121 ctgatgctgg gcaacgccta tctggatacc caggaacttc gtgcccgctc gctgacgtgg
     3181 gcggacgcgc tggccaaccg gaccggcgag gcggtgtggg tcgccgtgct caccggtgac
     3241 cacgtgttcg tcgtgcacca tgcgttccgg cccgagggcg cggtgcagat cctcgaggtc
     3301 ggcgcgagca tcccctggag cacctgcgct ctgggcaagg cgatcgtggc gttcctgcca
     3361 cctgaccacc gaaagtccct gctggccgcc gagcttccag cactgacggg gtggagcgtc
     3421 acggattctc aggccgtagc tgagcaactc gatgcggtcg tcaagtccgg ctacgcgctc
     3481 gaagatcagg agtcagctct cggagacgcc ggcatcgccg cccccgtctt tgaccgctcg
     3541 ggcggggtgg ccggagcgat cgggatcgtc ggtccgatcg agcggctttt cgcggattct
     3601 gcacgtcaag accatgttgt cgcggtgcgc gaagtagcac gccacctctc gcgcgagctg
     3661 ggcgcggggc gcggcgcgat gcgtggaatg cggctgtagg acggcccttc tcccggcgcg
     3721 agtcgggcgg cctgggagca aagggcaggt cgaggaagtc ggtccagctg tcggccgggc
     3781 gtccgcgtgt gccgttaggc cgcggcttct gtacatccac atcacgactc ggcttcgccg
     3841 ccacgcctcg gtcttgacgg ctgtcccagg cggggctagt ttccgaacat cgttcatcca
     3901 tactgaacgc gatctcgtca aaacgccgac gccctagcgg gtcggctgcg cacttcgccg
     3961 ggctgcgggc accgcggttc ggtccttccc caagtcgtct gctttcgcaa tgatgcgaag
     4021 gaggtggacg cccacaggcc tgcgggccgc gggcgcgtca tgccagaaaa cacgttcccc
     4081 caaaagctcc tcgcggagct gctcggcacg gcgatgctgg tgttcatcgg cgtcggctca
     4141 gtgcccgcga cactcatcat cggcggcaag gcaccgttca ccatggccga gctgggcatg
     4201 atctcgttcg cgttcgcgat ggtcgtcgtc gcgatgatct acgcgatcgg gcacatctcc
     4261 ggctgccaca tcaatcccgc catcaccctc gcgctggccg ccaccggcaa gctgccctgg
     4321 cgcgacgtgc ccggctacat cgccgcacag gtcgccggag cgacgctcgg cgcgttcgcc
     4381 atcgtcggcg tgctcggaca caaggccgtc gacgtcggat tgggcatcgc gtcctacagc
     4441 gccccggtcg gcgccggtcg cgctttcttc gccgaggcgg tcggcacgtt catcctcgcc
     4501 ttcgtggtgt tcggcgcgtt cgacggccgc gcgccgggcg gattcggcgg cgtcgcggtc
     4561 ggcctcgccg tcttcgcgat catcatcccg gtcggaccgg ccaccagcgc gtccatcaac
     4621 cccgctcgcg ccatcggtcc gatgctggtc gggcagttgt tcaacggcac ggtgcactgg
     4681 aaccagcttc cggtttatct ggccgctgag atcatcgggg ccgtcctcgc cggcctcacc
     4741 tacgtagcgc tcaacacaaa gcgcgctgcc gtgcgcagcg tcagcacgcc ccagcccgtg
     4801 ccggccgaag gagtctcggc atgaagaagc tgatcaacac gcccgaatca gtcctcgctg
     4861 acgcgctcgc cggaatcgcg gccgcgcacc cggacctcgc cgtggaccgc gacgccagga
     4921 tcatcacccg tgccagtggc cctaaccgcg gaaaggtcgc gctgatctcc ggcggtggat
     4981 ccgggcacga gccgctgcac ggcggcttcg tcggccccgg aatgctgaac gcggcgtgcc
     5041 ctggtgaggt gttcacctcc ccggtgcccg accagatgct ggccgccgcc cgcgccgccg
     5101 acgggggagc cggggtggtg ttcatcgtga agaactacac cggcgacgtg atgaacttcg
     5161 agatggcggc cgatctcgcc gccgacgacg ggatcagcgt cgccaccgtg ctcgtcgacg
     5221 atgacgtggc ggtcaaggac tcgacctgga ccgccgggcg gcgcggtacg ggagccacgg
     5281 tcctggtgga aaagatcgcc ggggcgctcg ccgaggccgg tggtgacctg gccgccgtcg
     5341 ccgagctggg caccagggtg aacaaggctt cgcgcagctt cgccgtcgcg ctcaccgcct
     5401 gcaccacccc agcggccggc aagccgggct tcgagcttgg cgaggacgag atggaggtcg
     5461 gcgtcggcat ccatggtgag ccgggacgca gccgcgaaaa gctccgcccg gccgcggaaa
     5521 tcgtcgccac cgcgctggac gcgatcctcg ccgaccacga gctcgatgcg ggtgacgagg
     5581 tcatcgtcct ggtcaacggg cttggcggta ctccgttgat cgagctctac gtcgccttcg
     5641 ccgaggtggc ggcgatcctg gcggcccagg gcgtgcgcat cgcccgcagc ctcgtcggga
     5701 actatgtcac cagtctcgac atggcgggcg tgtcgctgac cgtatgcaag gcggatgagc
     5761 agatgctcgc cttgtgggat gcgcccgtga acaccccggc gttgcgctgg ggcgtctaag
     5821 caggtccacg ggatcggccg tgccacgcgc ggccggtccc ggtcccaatc tcgatccacg
     5881 aatccac
//
LOCUS       DASUBI010000101         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1249884, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000101 DASUBI010000000
VERSION     DASUBI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249884"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1067
                     /locus_tag="VFU72_01955"
     CDS             123..1067
                     /locus_tag="VFU72_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422277.1"
                     /translation="MLAYTLRRLLLTIPVMLLVATGVFLLLHLTPGDPVGVMLGPDAS
                     EEQRLEMRHRLGLDRPLVVQYVDWLGRVVRGDLGTSLFLDKPVTTALLERAQPTLMLT
                     ILASLVSLLVGLPSGVIAARRRGSWLDLGSMAVAMLGISMPTFWLGLNLILLFAVHLR
                     VFPVAGYQPLSAGPWEALKYLVLPAVTLGVAQAALLARMTRSMMLEVLSQDYVRTARA
                     KGLNEHAVLVRHTLRNAFIPLLTVIGLIVAALLSGAVVTEQVFNIPGVGRLLIQAIGR
                     RDFPLVQGAVLVISAIYVLVNLAVDLLYAVIDPRIRQA"
     gene            1080..1973
                     /locus_tag="VFU72_01960"
     CDS             1080..1973
                     /locus_tag="VFU72_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422278.1"
                     /translation="MGAKVELSIERGGQAIRQARRRRNLDLVLKQKGIVIGGIILVLM
                     ALTALAAPLLTSHDPNLVIPLDRLKRPSAVHFFGTDGQGRDIYSRVLYGGRTSLLVGF
                     GVALVTLIGGTLIGLLAGYYPKVNAVVMRLMDGMMAFPSIILAIGIMAVRGADVSNVI
                     LALSIVAVPRLARLVRSVVLGLNDAPFVEAARAGGNTDSRIVWRYILPNCLSPLIVQV
                     SFVFAEAVLGEATLSFLGAGAPPDISSWGTMLSESKAFLQQAPWTMIFPGLALVLTVF
                     ALNLLGDGLRDVLDPRLQRRA"
     gene            complement(2183..>4577)
                     /locus_tag="VFU72_01965"
     CDS             complement(2183..>4577)
                     /locus_tag="VFU72_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5422279.1"
                     /translation="RGSALLLILLLALGAWSAPAGAANTNILTLTPSSQHALVGGSAQ
                     VTATLRTQGGVPVPSAFIQLSISGTMQETMLETTDSQGQATFSYTGVSAGVDMLVVSS
                     ATAQFGATATITWQATGQASSITLSPASLTAAVGTAPAFTATLVDPNGVPVPGVAVSF
                     SVSGVNSGSSTVLTNSQGQAVFSYLGTRIGQDTLTATAPGGVTPAAATIHWTNALQIT
                     ITPSALTAVAGTAQTFVATVTDGIGNPIPLATVTFSASGANSASGTDVSDSNGEASFT
                     YTGNHVGVDSLTATTPGAPVAALATISWVSTAAISLSPTNVSAAVGTAQTITATVVDL
                     DGDPIPGVVVSFSVSGVNAASGSVTTDSDGEASFSYTGAHVGTDTVSATTPGVQVAAT
                     ATIVWSVAESVSISPETVTVATGVVQSFTATVVDVDGDPVPGEVVMFTVTGANPTSGT
                     ATTNSDGQASFSYNGTASGTDTVTAFVDLNNNGSAGSNEPAAKAVVTRTSASLALSVS
                     SASATTGASVTVTATLSQTAGALAGRTIRFTVSGANPTSGSGTTNSSGQATFSYAGAT
                     AGSDAITAYADLNANGSRDAGEPSATAAVAWSTPPKPALPAAEPAAPQAGCTYFAVTR
                     HNLCAGFASYWNKFGGLAVYGYPLTEEFVENGFTVQYFERARFEWHPGAWPERYDVEL
                     GLLGNMVTVGRGGEPPFQRAASKPDTSCVYYAETGHNLCGGFGDFWQQFGGLAVYGFP
                     ISEEFQEKNADTGKTYTVQYFQRGRFEWHPGEDPQHFDVMLGRLGAEILNATYGTKF"
BASE COUNT          623 a         1571 c         1587 g          796 t
ORIGIN      
        1 acccggccga ctggttcttc tggaacgtcg gtatcgccta agcgcgctcg cggcacggcg
       61 agccgggcgc tcacccggcc cgccgctgcc tgccgcgcca caggtcgaac gggagagggt
      121 tgatgctggc ctatacccta cgccgcctgc tgctgacgat tccggtgatg ctcctggtcg
      181 ccaccggcgt cttcctgctg ctccacctca cgcccggcga tccggtgggg gtcatgctcg
      241 ggccggatgc ctcggaggag cagcggcttg agatgcgcca ccggctgggg ctcgaccggc
      301 ccctggtggt gcaatacgtc gactggctcg ggcgggtggt gcgcggcgat ctcggcacct
      361 cgctcttcct cgataagccg gtgaccaccg cgctgctgga gcgcgcccag ccgaccctga
      421 tgctgacgat cctggcttcg ctcgtttcgc tcctggtggg gctgccgagc ggggtcatcg
      481 cggcgcggcg gcgcggctcc tggctcgacc tcggctcgat ggccgtcgcc atgctcggca
      541 tctcgatgcc caccttctgg ctcggcctga acctgatcct cctcttcgcc gtccatttgc
      601 gcgtcttccc ggtggccggc taccagccgc tctcggccgg tccctgggaa gcgctgaagt
      661 acctggttct gcccgccgtg acgctcggcg tggcccaggc cgcgctgctg gcgcgcatga
      721 cccgctcgat gatgctggag gttctgagtc aggactacgt gcgcacggcg cgggccaagg
      781 ggctcaatga gcacgcggtc ttggtgcggc acacgctgcg caacgccttc atcccactcc
      841 tgacggtgat cggtctgatc gtggccgcgc tcctctccgg cgcggtggtc accgagcagg
      901 tgttcaacat tcccggtgtc gggcggctct tgatccaggc gatcggccgc cgcgatttcc
      961 cactggtgca gggcgcggtg ctggtcatct cggcgatcta cgtcctggtc aacctcgccg
     1021 tcgatctcct ctacgcggtg atcgacccgc gcatccggca ggcctaagga gcgcttggag
     1081 tgggggctaa agtggaactc tcgatcgaac gcggcggcca ggcgatccgc caggcgcgcc
     1141 ggcggcgcaa cctcgacctg gtgctcaaac agaaggggat cgtgatcggg gggatcatcc
     1201 tcgtgctgat ggccctgacc gccctggcgg ccccgctcct gaccagccac gaccccaatc
     1261 tggtcatccc gctcgatcga ctcaagcgcc cgagcgccgt tcatttcttc ggcaccgacg
     1321 gccaggggcg cgacatctac agccgcgtgt tgtatggcgg gcgcacctcg ctcctggtcg
     1381 gctttggcgt cgccctcgtc accctgatcg gggggaccct gatcgggctg ctggccggct
     1441 attacccgaa agtcaacgcg gtcgtgatgc ggctgatgga cggcatgatg gccttcccca
     1501 gcatcatcct ggcgatcggc atcatggccg tgcgcggcgc ggacgtctcg aacgtcatcc
     1561 tggcgctcag tatcgtggcc gtgccgcgtt tggcccggtt ggtgcgcagc gtcgtgcttg
     1621 ggctgaacga cgcccccttc gttgaggcgg cacgggccgg cggcaacacc gacagccgca
     1681 tcgtttggcg ctacattctc cccaactgcc tgtcgccgct gatcgtgcag gtcagcttcg
     1741 tcttcgctga ggcggtgctg ggggaggcga cactctcctt cctgggcgcg ggggcgccgc
     1801 cggacatctc ctcctggggc acgatgctga gtgagtccaa ggccttcctg cagcaggcgc
     1861 cctggaccat gatctttccc ggcctggcgc tggtgctgac cgtcttcgcc ctcaacctgc
     1921 tgggcgacgg cctacgcgac gtcctcgacc cgcgcttgca gcggcgggca taagccggcg
     1981 cgcctcatca tcaccggtag gggcaggccc ccgtgcctgc cattgttgcc cctagcgccg
     2041 ggcccgcatc aacgtcaacc aacgtacccc atgcccccca gccggtgcgt ggggggatga
     2101 gggcgaccac gggggggtcg cccctacaag ggcagacacg gggcaaccgc tccaggtcac
     2161 ccgctcctcg gcgacgtgac ggctagaact tggtgccgta ggtcgcgttg aggatctccg
     2221 cgccgaggcg gcccagcatc acgtcgaaat gctgcggatc ctcacccggg tgccactcga
     2281 aacggccccg ctggaagtac tgcaccgtgt aggtcttgcc ggtgtcggcg ttcttctcct
     2341 ggaactcctc gctgatcggg aacccgtaga cggccaggcc gccgaactgc tgccagaagt
     2401 cgccgaaccc gccacacagg ttgtggccgg tctccgcgta gtagacgcac gaggtgtccg
     2461 gcttggaggc ggccctctgg aagggcggct caccaccgcg accgaccgtc accatgttac
     2521 ccaagaggcc cagctcgaca tcgtagcgct ccggccaggc tcccgggtgc cactcgaagc
     2581 gcgcccgctc gaagtactgg acggtgaagc cattctcgac gaactcctcg gtcaggggat
     2641 agccgtagac cgccaggccg ccgaacttgt tccagtagga ggcgaaaccc gcgcacaggt
     2701 tgtggcgcgt gaccgcgaag taggtgcagc ccgcctgcgg cgccgccggc tccgccgccg
     2761 gtagcgccgg cttgggcggg gtcgaccagg cgaccgcggc tgtcgccgac ggctcgcccg
     2821 cgtcccggct gccgttggcg ttgagatcgg cgtaagcggt gatcgcgtcg ctgcccgccg
     2881 tggccccggc gtagctgaag gtcgcctggc cgctgctatt ggtggtcccg ctgccgctgg
     2941 tcgggttcgc gccggagacc gtgaagcgga tcgtccgccc ggcaagagcg ccggctgtct
     3001 gggagagcgt cgcggtcacc gtgacgctgg cgccggtcgt ggcgctggcg ctggagacgg
     3061 agagtgccag gctggcgctg gtgcgggtca cgaccgcctt cgccgctggt tcgttgctgc
     3121 ccgcgctgcc gttgttgttc agatccacga aggccgtcac ggtatcggtc ccgctcgcgg
     3181 tcccgttgta gctgaagctg gcctgcccgt cgctgttggt ggtcgccgtc ccactggtcg
     3241 ggttggcgcc cgtgacggtg aacatcacga cctcgccggg cacgggatcg ccgtcgacgt
     3301 ccaccacggt ggcggtgaag gactgcacca ccccagtcgc cacggtgacc gtctcagggc
     3361 tgatcgagac actctcggcc accgaccaga caatcgtcgc cgtggcggcc acctgcacac
     3421 cgggcgtggt cgcgctcacc gtgtcggttc ccacgtgtgc cccggtgtag ctgaagctgg
     3481 cctcgccgtc actatcggtg gtcacgctgc cgctggcggc gttgacaccg ctgaccgaga
     3541 agctgaccac gactcccgga atgggatcgc cgtcgagatc gaccacggta gcggtgatcg
     3601 tctgtgcggt gccgacggcg gccgagacgt tcgtcgggct gagggagatg gcggcggttg
     3661 agacccagga gatcgtcgcc agcgcggcca ccggcgctcc gggggtggtc gcggtcaggc
     3721 tatcgacacc cacgtgattg ccggtgtagg tgaagctcgc ctcgccgttg ctgtcgctca
     3781 cgtcggtgcc gctggcgctg ttggcgccgc tcgcgctgaa ggtcaccgtc gccagcggaa
     3841 tgggattccc aatcccatcg gtcaccgtgg ccacgaaggt ctgagcggtg ccggcgacgg
     3901 ccgtcagcgc gctcggggtg atcgtgatct gcaaggcgtt ggtccagtgg atggtcgccg
     3961 ccgcgggggt gacgccgccc ggcgccgtcg ccgtcagggt atcctggcca atgcgggtgc
     4021 cgaggtagct gaagaccgcc tggccctggc tattggtcag caccgtgcta ctgcccgaat
     4081 tgactcccga gacagagaaa ctgacggcga ccccggggac cgggacaccg ttcgggtcga
     4141 ccagggtggc cgtaaaggcc ggcgccgtgc cgacggccgc cgtcaggctg gccgggctca
     4201 gggtaatgga gctggcctgc cccgtggcct gccaggtgat ggtcgcggtc gccccgaatt
     4261 gggccgtcgc gctggacacg accagcatgt ccaccccggc gctcacgccg gtgtagctaa
     4321 aggttgcctg cccctggctg tcagtggttt ctaacatcgt ctcctgcatc gtccccgaga
     4381 tcgagagctg aatgaacgcg ctgggcaccg gcacgccgcc ctgggtgcgg agggtggcgg
     4441 tgacctgggc cgatccgccg accagcgcgt gctgcgagct gggagtgagg gtcaggatat
     4501 tcgtattggc cgcgccggcc ggcgcggacc aggcgccgag cgcgagcagc aggatgagca
     4561 gcagcgccga gccgcgc
//
LOCUS       DASUBI010000102         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_23822, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000102 DASUBI010000000
VERSION     DASUBI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23822"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFU72_01970"
     CDS             <1..1149
                     /locus_tag="VFU72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5422280.1"
                     /translation="DDQVMLRNLLREFLTERGPVAHVRAMMETPLGYDPAIYRELIQL
                     GALPFPEAYGGSGLGMVEQAIVLEEMGRIPYPGPYLATVILAGGAIMASGDENAQARY
                     LPDAANGDLTMTLAFLENSIGYGPNEVQLTAKRDGDSYVLNGIKRFVPYGQSADVILV
                     AARSGAGAGPEGVSLLAVPRETAGLSVEPEVTLDLTTRTATLHFKDVRVPAENLIGGE
                     GGAWPAIAQAHTLAAIGAAAEQLGASRKSLEMATEYAKVRKQFGQFIGQFQAVKHMLA
                     EMLEQVETSFGAVYYAAWAQDADAPDAALAASVAKATLNQASRKVCGDAIQAHGGIGF
                     TWEYDLHLYFKRAKHYEPFYGDTEYHRERVLEEFLAGHTAGVTAGGAA"
     gene            1146..1640
                     /locus_tag="VFU72_01975"
     CDS             1146..1640
                     /locus_tag="VFU72_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5422281.1"
                     /translation="MSGAETGQVITTVDELRQWVGKETGVSDWLEITQERVNLFADAT
                     GDHQYIHVDPERAKQTFFGGTIAHGYLTLSLIPYLGSLKVEGPRINLGGRMGVNYGLN
                     RVRFPAPVPVGSRIRMHTRLVKVEEIEGGSGRAVQTTNEQTIEVEGQERPACVAETLG
                     RTYF"
     gene            1805..4084
                     /locus_tag="VFU72_01980"
     CDS             1805..4084
                     /locus_tag="VFU72_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5422282.1"
                     /translation="MAPNVVDGQGHLMTTAQPERTAVAGEEKPAAESDAFHTAMRALF
                     HPERVAIVGATPKPGFASAIHRNILRHYQGEVVGITPRYPEVFGSPCYPALTDVPGGV
                     DSAILVVPSQIVMEILAQAEQAGVKALNIITSGFGEQADAEAHARQDEIKRFAERTGI
                     RIVGPNCLGLISTPANLIAMSGPYTDIKRGPIAIVFQSGLLTYSVTIPQQDRDFGYSY
                     VITTGNEADLDATDFMRYCVEDDETQVVGCFIEQFRSPEKLREVAQLAAERQKPIVVL
                     KIGRSEAGERAARAHTGSLVGSDAIADAVMRQYGIVRVSSLDEMIETLAVFHSQRLPK
                     GPGVGTIFVSGGAGGMISDLAQDLGVSLPELAPETVAKLQPVIPVYGTVGNPLDTTGQ
                     AAQQPEIMQGSLTAMAEDPNIHTIVYGQAFPTRIDLATPVGQVLKAIPERYPDKVFVA
                     LALVTGRVAEGRQRDQEPEEPAMAWNGVPFLQGAENGLRAIRALNRYAEFLRQRERGQ
                     AGAEKDHERALAARALVRAGGGKPLVERAAKEVLALYGIPVTRERLATTADDAVAAAE
                     TIGYPVVLKIESPDIAHKTEAGGVLLDLRDQAAVRDGFARVLANAQHHDPRAQLTGVV
                     VQEQVGRGHELILGMTQDPGFGPAIAVGLGGIFVETLNDVTLGVPPLSARDARSMLAR
                     LRAAPVLEGRGSRGAGPADTEAVVEILRRFSQLALDLDGDVSEIDINPLVVFEQGQGA
                     RVVDCLIVPASPAWSGAEA"
     gene            4081..>4289
                     /locus_tag="VFU72_01985"
     CDS             4081..>4289
                     /locus_tag="VFU72_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5422283.1"
                     /translation="MTELGEIVSLISDVHGAAGTLERALAACRREGVETVALLGDLID
                     RPEQADACALVLAQWRVVGVYGNHER"
BASE COUNT          683 a         1485 c         1517 g          604 t
ORIGIN      
        1 gacgaccagg tcatgctgcg caatctgttg cgtgagttcc tgaccgagcg cggtccggtc
       61 gcccacgtgc gcgccatgat ggagaccccg ctgggctacg acccggcgat ctatcgtgag
      121 ttgattcagc tcggcgcgct ccccttcccc gaggcctacg gcggctcggg gctggggatg
      181 gtcgagcagg cgatcgtgct ggaggagatg ggccgcattc cctaccccgg tccctacctc
      241 gcgaccgtca ttctggccgg cggcgccatc atggcctcgg gcgacgagaa cgcccaggcg
      301 cgctacctgc cggacgccgc caacggcgac ctgacgatga cgctcgcctt tctggagaac
      361 tcgatcggtt acggcccgaa cgaggtccag ctcacggcga agcgcgacgg cgacagctac
      421 gtgctcaacg ggatcaagcg cttcgtgccc tacggccaga gcgccgacgt catcctggtc
      481 gccgcgcgca gcggcgcggg agcggggccc gagggcgtct cgctcttggc cgtgccgcgt
      541 gagaccgccg gcctgagcgt cgagccggag gtgacgctcg acctgacgac ccgcaccgcc
      601 acgctccact tcaaggacgt gcgcgtgccg gccgagaacc tgatcggcgg cgagggcggc
      661 gcctggccgg cgatcgcgca ggcgcacacg ctggccgcca tcggggcggc cgccgagcag
      721 ctcggcgcct cgcgcaagtc gctggagatg gcgaccgagt acgccaaggt ccgcaagcag
      781 ttcggccagt tcatcggcca gttccaggcg gtcaagcaca tgctggccga gatgctggag
      841 caggtggaga cgagcttcgg cgcggtctac tacgccgcct gggcgcagga cgcggacgcg
      901 cccgacgccg cgctggccgc ttcggtcgcc aaggcgacgc tcaaccaggc ctcgcgcaag
      961 gtctgcggcg acgccatcca ggcgcacggc ggcatcggct tcacctggga gtacgacctg
     1021 catctctact tcaagcgcgc caagcactac gaacccttct acggagacac cgagtatcac
     1081 cgcgagcgcg tgctggagga gttcctggcc ggacacacgg ccggagtgac ggcgggcggt
     1141 gcggcgtgag cggcgccgag accgggcagg tcatcaccac cgtcgatgag ctgcgccagt
     1201 gggtcggcaa ggagaccggc gtcagcgact ggctggagat cacccaggag cgcgtcaatc
     1261 tgttcgccga cgccaccggc gatcaccagt acatccacgt cgacccggag cgggccaagc
     1321 agaccttctt cggcgggacg atcgcccacg gctacctgac gctctcgctg atcccctacc
     1381 tcggcagcct gaaggtcgaa gggccgcgca tcaacctggg cggccgcatg ggggtgaact
     1441 acgggctcaa ccgggtgcgc ttcccggcgc cggtgccggt cggcagccgc atccggatgc
     1501 atacgcggct ggtcaaggtc gaggagatcg agggcggctc cgggcgagcc gtgcagacga
     1561 ccaacgagca gacgatcgag gtcgagggcc aggaacgtcc ggcctgcgtg gccgagacgc
     1621 tggggcggac gtatttctag gaacgggcgg cggacgagga acatttggtg ccgggagagg
     1681 acggatcctt cgacaagctc aggatgaccg ggtgaatacg gggcctggcg ggagagactc
     1741 atcctcgtcg tcagccccat ccctatccct gtcatcctga gcgcagtcga aggatccgtc
     1801 ctggatggca ccgaacgttg tcgatgggca aggacacctg atgacgacag cgcagccgga
     1861 acggacggcg gtggccggtg aggagaaacc ggcggccgag agcgatgcct ttcataccgc
     1921 gatgcgcgcg ctctttcatc ccgagcgcgt cgcgatcgtc ggggcgacgc ccaagccggg
     1981 cttcgccagc gcgatccacc gcaatatcct gcgccactat cagggtgagg tcgtcggcat
     2041 cacgccccgc tatcccgagg tcttcggctc gccttgctac ccggcgctga ccgacgtgcc
     2101 gggcggagtc gactcggcca tcctggtcgt gccgagccag atcgtgatgg agattctggc
     2161 ccaggccgag caggccgggg tcaaggcgct caacatcatc accagcggct tcggcgagca
     2221 ggccgacgcg gaggcgcacg cgcgccagga cgagatcaag cgcttcgccg agcgcaccgg
     2281 catccggata gttggtccca actgcctggg gctgatcagc accccggcca acctgatcgc
     2341 catgtccggc ccctacaccg acatcaagcg cgggccgatc gcgatcgtct tccagagcgg
     2401 gctgctgacc tacagcgtca ccattcccca gcaggaccgc gacttcggct actcctacgt
     2461 catcaccacc ggcaacgagg ccgatctcga cgccaccgac ttcatgcgct actgcgtcga
     2521 ggacgacgag acgcaggtgg tcggctgctt catcgagcag ttccgctcgc cggagaagct
     2581 gcgtgaggtg gcccagctcg ccgccgagcg ccagaagccg atcgtcgtcc tcaagatcgg
     2641 ccgctccgag gcgggcgagc gtgccgctcg cgcccacacc ggctcgctgg ttggctccga
     2701 cgccatcgcc gacgcggtga tgcgccagta cggcatcgtg cgcgtctcca gtctcgacga
     2761 gatgatcgag acgctggcgg tcttccatag ccagcggctg cccaaaggcc ccggcgtcgg
     2821 caccatcttc gtctcgggcg gcgcgggggg catgatctcc gatctggcgc aggacctcgg
     2881 cgtgtcgctg ccggaattgg cgccggagac ggtcgccaag ctacagccgg tcatcccggt
     2941 ctacggcacg gtcggcaatc cgctggacac caccggccag gccgcccagc agccggagat
     3001 catgcagggc tcgctgaccg ccatggccga ggacccgaac atccacacca tcgtctacgg
     3061 ccaggccttc cccacgcgca tcgacctggc gaccccggtc ggccaggtgc tcaaggcgat
     3121 ccccgagcgc tacccggaca aggtcttcgt cgccctggcg ctggtgaccg gaagggtggc
     3181 cgaggggcgg cagcgcgacc aggagcctga ggagccggcc atggcctgga acggcgtgcc
     3241 cttcctgcag ggcgccgaga acgggctgcg cgccatccgc gcgctcaacc gctacgccga
     3301 gttcctgcgc cagcgggagc gcggccaggc cggcgcggaa aaggatcacg agcgtgccct
     3361 ggccgcgcgg gcgctggtgc gcgcgggcgg cggcaagccg ctggtcgagc gtgccgcgaa
     3421 agaagtgctg gcgctctacg gcatcccggt gacgcgcgag cgcctggcga cgaccgccga
     3481 cgacgcggtc gcggcggccg agaccatcgg ctatccggtc gtgctcaaga tcgagtcgcc
     3541 cgacatcgcc cacaagaccg aggcgggcgg ggtgctgctc gacctgcgcg accaggccgc
     3601 cgtgcgcgac ggcttcgcgc gagtgctcgc gaacgcccag caccacgacc cgcgcgccca
     3661 gctcaccggc gtggtggtgc aggagcaggt cgggcgcggc cacgagctga tcctgggcat
     3721 gacccaggat cccggcttcg gtccggccat cgcggtcggg ctgggcggca tcttcgtcga
     3781 gaccctcaac gacgtcacgc tcggggtgcc gccgctctcg gcgcgcgacg cccgctcgat
     3841 gctcgcgcgc ctgcgcgcgg cgccggtgct ggaggggcgc ggctcgcgcg gcgcgggccc
     3901 ggcggacacc gaggccgtcg tcgagatcct gcggcgcttt tcgcagctgg cgctcgacct
     3961 ggacggcgat gtatccgaga tcgacatcaa ccccctggtc gtcttcgagc agggccaggg
     4021 tgccagggtc gtggactgct tgatcgtgcc ggcgtctccg gcctggagtg gcgcggaggc
     4081 gtgacggagc tgggagagat cgtctcgctg atctcggatg tccacggcgc ggcgggaacc
     4141 ctggagcgcg cgctggcggc gtgccggcgg gagggagtgg agacagtcgc cttgctaggg
     4201 gatctcatcg accgcccgga gcaggccgac gcctgcgcgc tggtcctggc gcagtggcgc
     4261 gtcgtcggcg tctacggcaa ccacgagcg
//
LOCUS       DASUBI010000103         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1223218, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000103 DASUBI010000000
VERSION     DASUBI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223218"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFU72_01990"
     CDS             <1..575
                     /locus_tag="VFU72_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422284.1"
                     /translation="VYAGSGAATTVDSGALKPQINSWHYNWQPHGAPIGYAAIDARLR
                     AGEPLAVAELEPDRIAVREPGSLTAAQPAPAGHVAAFGGLWLLNADVSRDGDHLTVVS
                     RWYVSRPILGDPRLRVELRAAGGKVLAARTGYALDGFSPPRLWQPGDLIEDREVFALP
                     GTRDPAGARIGLVDTSGALLGGWVTAGSTP"
     gene            complement(780..1406)
                     /locus_tag="VFU72_01995"
     CDS             complement(780..1406)
                     /locus_tag="VFU72_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422285.1"
                     /translation="MSEIVPQSQPPPEEEDNEPGDERALVEYTGEAEIAGVGDTLRDV
                     GSRLPAYARLSQGLVAAGLLSANQQADILGPLGYGPLARVARFVPLLNQLTRILSLVG
                     AIRFALTQLDPELAEEHLARAGLSRAQVEADFQATRGLAERIAASGSQQAAHAYELGK
                     EQAARLVEASGPIASKALEDGARAAGKLTGKGLRAWRNWQAKSNERKG"
     gene            complement(1464..>3198)
                     /locus_tag="VFU72_02000"
     CDS             complement(1464..>3198)
                     /locus_tag="VFU72_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422286.1"
                     /translation="YVTALSDGALRVAASGGASGGSYSDGKRIFFPIELDLFDEPDAA
                     RRFYRLNAAWQVLQVRSGALEIAPLLAGADPGRALALYEALAGEWLDRRLAATWPGLA
                     ADLTWLRADALGRRPAPEHPVEALQQALLAQPLDRLGELALPALALDARALSEVLSAD
                     DPAPARELAAALASQLPPLTGSVPTVHYRGRIRVELVRTWSPEAAAEPEEEAVPAPGP
                     PENRRRREPLVVDIRPIEQRIQRERRAKAGGRHAVQLSLGDREPTITAAQVPLSESER
                     SDALLYDEWDYRRDDYLREWCAVHLRHPRGGSDDAVERILRQHAALINRLKQQFEALR
                     PERQRLTRQLDGDELDLNALVEDHADRRAGFSPGEKLYSRTLERQRDIALGCLIDLSG
                     STGAWIDDDPRNDQVIEVTRRAVVFLCEALTTLEDRYALFGFTSGTRKRCEIAVLKGF
                     EESYGQTVKGRLAGMAPGSYTRIGPAVRHTAHALTQQPAKLRLLLLVSDGRPNDFDGY
                     GGRYGIEDTRRALIEARRQGIATFALTIDAEARDYMPYMFGRNHYVVVENAPALATRL
                     ADIYRRLTVPQ"
BASE COUNT          402 a         1145 c         1199 g          452 t
ORIGIN      
        1 tggtctacgc cggctccggc gccgccacca cggtcgactc cggcgcgctc aagccccaga
       61 tcaacagctg gcactacaac tggcagccgc acggcgcacc gatcggctat gcggccatcg
      121 acgcccggct gcgcgcgggc gagccgctgg ccgtggccga gctggagccg gatcgtatcg
      181 ccgtgcgtga gccgggcagc ctgacagccg ctcagcccgc gccggccggg cacgtcgccg
      241 cgttcggcgg cctctggctg ctcaacgcgg atgtcagccg cgacggcgat cacctcaccg
      301 tcgtcagccg ctggtacgtc tctcgtccga tcctcggcga tccgcgcctg cgcgtcgagc
      361 tgcgcgctgc cggcggcaag gtgctggccg cgcgcaccgg ctacgcgctc gacggcttct
      421 ccccgccgcg cctgtggcag ccgggtgacc tgatcgagga ccgcgaggtc ttcgcgctgc
      481 cgggcacgcg cgatccggct ggggcccgca ttgggctggt ggatacgagc ggtgcgctgc
      541 tgggcggctg ggtgacggcg gggagcaccc cgtgatgggg ggtgctacgg cgttcgagga
      601 ggttgagcgc cgggtgggga cggatccttc gactgcgctc aggatgacag ggctaaggag
      661 gggcggacga cacgcatcgt tctcgtcgtc agcctcatcc ctcaccctgt catcccgacc
      721 gaagtggagg gatccgtcct cgatggcacc cgggcgcgtc gaagagcagc gcttcgctct
      781 cagcccttgc gctcgttcga cttcgcctgc cagttgcgcc aggcgcgcag gcccttgccg
      841 gtcagcttgc cggcggcgcg ggcgccgtcc tccagcgcct tcgaagcgat cgggccgctc
      901 gcctcgacca gccgggcggc ctgctccttg ccgagctcgt aggcgtgggc ggcctgctgg
      961 ctgccactgg cggcgatgcg ctcggccagg ccacgcgtcg cctggaagtc ggcctccacc
     1021 tgggcgcggc tcaggccggc ccgcgccagg tgctcctcgg ccagctccgg atcgagctgg
     1081 gtcagcgcga agcggatggc gccgaccagc gagaggatgc gcgtgagctg gttgagcagc
     1141 ggcacgaagc gcgcgacgcg cgccagcggc ccgtagccga gcggcccgag gatgtcggcc
     1201 tgctggttgg ccgacagcag cccggcggcc accagcccct gcgagagccg cgcgtaggcc
     1261 ggcaggcgcg agccgacgtc gcgcagcgtg tcgccgacgc cggcaatctc agcctcgccg
     1321 gtgtactcaa ccagcgcgcg ctcgtcgccg ggctcgttgt cctcctcctc tggaggcggc
     1381 tggctctggg gcacgatctc gctcatcaga ctccactctc caggcgcatg ctcacctgaa
     1441 gtctaccgtc tgacggaagg gggctactgc ggcacggtca ggcggcggta gatgtcggcc
     1501 agccgcgtgg ccagcgcggg cgcgttctcg acgacgacat agtggttacg gccaaacatg
     1561 tagggcatgt agtcgcgcgc ctcggcgtcg atcgtcaggg cgaaggtggc gatcccctgc
     1621 cggcgcgcct cgatgagcgc gcggcgggtg tcctcgatgc cgtagcgccc gccgtagccg
     1681 tcgaagtcgt tgggccgtcc gtccgagacc agcagcagga gccgcagctt ggccggctgc
     1741 tgcgtcaggg cgtgcgcggt gtggcgcact gccgggccga tgcgcgtgta ggagccgggc
     1801 gccatgccgg ccaggcggcc cttgaccgtc tggccgtagc tctcctcgaa gcccttcagg
     1861 accgcgatct cgcagcgctt gcgcgtgccg ctggtgaagc cgaagagggc gtagcggtcc
     1921 tccagcgtgg tcagcgcctc acagaggaag acgacggcgc ggcgggtgac ctcgatcacc
     1981 tgatcgttgc gcgggtcgtc gtcgatccag gcgccagtcg agccggagag gtcgatcagg
     2041 cagccgagcg cgatgtcgcg ctggcgctcc agcgtgcggc tatagagctt ctcgcccggc
     2101 gagaagccgg cgcggcggtc agcgtggtcc tcgaccagcg cgttgaggtc cagctcgtcg
     2161 ccgtcgagct ggcgcgtcag ccgctggcgc tcggggcgca gcgcctcgaa ctgctgcttc
     2221 agccggttga tcagcgccgc gtgctggcgc aggatccgct cgaccgcgtc gtcactgccg
     2281 ccgcgcgggt ggcgcaggtg gacggcgcac cactcgcgca ggtagtcgtc gcggcggtag
     2341 tcccactcgt catagagaag ggcgtcgctc cgctcggact cgctgagcgg cacctgggcg
     2401 gcggtgatgg tcggctcgcg gtcgcccagg ctgagctgca ccgcgtgccg cccgcccgcc
     2461 ttggcgcggc gctcgcgctg gatgcgctgc tcgatcggcc ggatatcgac caccagcggc
     2521 tcgcggcggc gccggttctc cggcgggccg ggcgcgggaa ccgcctcttc ctccggctcg
     2581 gccgccgcct cgggcgacca ggtgcgcacc agctcgaccc ggatgcgccc gcggtagtgg
     2641 acggtgggca cggagcccgt cagtggcggc agctgcgatg ccagcgcggc ggccagctcg
     2701 cgggcggggg ctggatcgtc cgcggacagc acctccgaga gcgcccgcgc gtcgagcgcg
     2761 agagccggca gcgccagttc gccgaggcgg tccagcggct gggccaatag cgcctgctgc
     2821 agcgcctcga ccgggtgctc cggagccggg cggcgaccca gcgcgtcggc gcgcagccag
     2881 gtcaggtcgg cggccagccc cggccaggtc gcggcaagcc gccggtcgag ccactcgccg
     2941 gccagcgcct cgtagagcgc cagcgcgcgg ccgggatcgg cgccggccag cagcggcgcg
     3001 atctccaggg caccactgcg cacctggagc acctgccagg ccgcgttcag gcggtagaag
     3061 cggcgcgcgg cgtccggctc gtcgaagaga tcgagctcga tcgggaagaa gatgcgcttg
     3121 ccgtcgctgt agctgccgcc gctcgccccg ccgctggcgg ccacccgcag cgcgccgtcc
     3181 gagagggcgg tgacgtag
//
LOCUS       DASUBI010000104         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_37213, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000104 DASUBI010000000
VERSION     DASUBI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37213"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFU72_02005"
     CDS             <1..928
                     /locus_tag="VFU72_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5422287.1"
                     /translation="FGAAAPAFDAVTTFGRPGERQLPGALLVERQMLTPDPAQPRKVF
                     DQEKLEDLAESIRQVGLLQPLRVRPGEDGKYIIVNGERRWRACMLADVPEIPVVVRDS
                     PADELAFEMLVENLQREDLSDEEEAEAYRTLIDQRYSVRQIAARLGVSIGRISKKVRV
                     YEDDALAEPILRGEMTGSQAEEILVAPAALRAPLAQTVVEARKAGSVVPLKELRATVR
                     EARDALDAGHAVEEVMESVSARNTFRTPAAVSAPANPHRTIDIAVKNLVGKIHGVIGA
                     YPDAPMSSEVSAALDEALAALTAWRQRNGVID"
     gene            1069..1938
                     /locus_tag="VFU72_02010"
     CDS             1069..1938
                     /locus_tag="VFU72_02010"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5422288.1"
                     /translation="MSELHGILPIVYTPFDATGEIIEADVRRLVDHLIAAGAHGLAAC
                     GGASEALKLTLAERRRLATLVIEQAAGRVPVIVGVSAATTHDAAALARHARDLGAQAV
                     FATPAQGAVTAAALHDHYAALAAAGAPVMVQDAEVSVTPEHMAELAAAIPGVRYVKEE
                     APTETGHRISAIRQLTDDRLLVLSGGTYLLDDLARGAVGAIPGSIGVADLVQAYERWR
                     AGDLVSARRAYDHFTPLSFFRRPFPLLAAKEVLRRQGVFSAAYLRAPAQHMLDAQDLA
                     ELTVVMATMGPPY"
BASE COUNT          345 a          724 c          725 g          328 t
ORIGIN      
        1 gttcggcgcg gccgccccgg cgttcgacgc cgtcacgacc ttcggccggc ccggcgagcg
       61 acaactcccc ggcgccctgc tcgtcgaacg gcagatgctg acgccggacc ccgctcaacc
      121 ccggaaagta ttcgaccagg agaagctcga ggatctggcc gagtcgatcc gccaggtggg
      181 tctgctgcag ccattgcgtg tccgccccgg cgaggacggc aaatacatca tcgtcaacgg
      241 cgagcgccgc tggcgggctt gtatgctcgc cgacgtgccc gaaatcccgg tggtcgtccg
      301 cgactcgccg gcggacgaac tggccttcga gatgctcgtc gagaacttgc agcgcgagga
      361 tctgagcgat gaggaagagg ccgaggccta ccgcacgctg attgaccaac gttacagcgt
      421 gcggcagatc gccgcgcggc tcggcgtgag catcgggcga attagcaaga aggtgcgcgt
      481 ctacgaagac gatgccctgg ctgagccgat cttgcgtggg gagatgactg gcagccaagc
      541 agaagagatc ctcgtcgcgc cggccgcgct gcgcgcgccg ctcgcgcaga ccgtggtcga
      601 ggcccgcaag gccggcagcg tggtgccgct caaggagttg cgcgcgaccg tccgcgaggc
      661 gcgcgacgcg ctcgacgccg ggcacgcggt cgaagaagtt atggaaagtg tttcggcccg
      721 aaacaccttc cgaactccgg cagcggtgtc tgcgccggcg aatccacacc ggacgatcga
      781 cattgcggtt aagaacctgg ttggcaagat ccacggcgtc atcggcgcct atcccgacgc
      841 gccgatgtcc agcgaggtca gtgccgcact ggacgaagcc ctcgccgccc tcacggcctg
      901 gcgccagcgc aacggcgtga tcgactgacg cgccgtgtga gtgtgcgtga gacgatgcca
      961 aaggtgaagg gggaaatatc cctgctcgca cacgctctcc actaggtgag cgcggtccga
     1021 gtaccgcgat ctggcgaccc ggcgcgcgac gaggaaaggg cgagcagggt gtcggaactc
     1081 cacgggattc tgcccatcgt ctatacgcca ttcgacgcca ccggcgagat cattgaggcc
     1141 gatgtgcggc ggctggtgga ccacctcatc gccgccggcg cgcacggtct ggcggcgtgt
     1201 ggaggcgcca gtgaggcgct gaaactaacg ctcgctgagc gccgtcggtt ggctacgctt
     1261 gttattgagc aggcggccgg gcgagtgccg gtaattgtcg gcgtcagcgc cgcgacgacg
     1321 catgatgccg ccgcgctcgc gcgccatgcc cgcgacctcg gggcgcaggc ggtctttgcc
     1381 acccccgccc agggagcagt aaccgcagcg gcgctccacg accactatgc ggcgctggct
     1441 gcggctggcg caccggtgat ggtccaggat gccgaggtca gtgtcacgcc ggagcacatg
     1501 gcagagttgg ccgccgctat ccccggcgtg cgctacgtga aggaagaggc cccaacggaa
     1561 acgggacacc gcatcagcgc gatccggcag ttgaccgacg accgcctgct cgtgctgtcc
     1621 ggcggcacct acctgctgga tgacctggca cgtggcgcgg tcggcgcgat ccccggcagc
     1681 attggcgtcg ccgacctggt ccaggcctac gaacgctggc gcgctggtga cctggttagc
     1741 gcgcggcgcg cctacgatca tttcacgccg ctgtcgttct tccgccgccc cttcccgctg
     1801 ctcgcggcca aagaggtgtt gcggcggcag ggggtgttca gcgccgcgta tctgcgtgcg
     1861 cccgctcagc atatgctgga cgcccaggat ctggccgagc tgaccgtagt tatggcgacg
     1921 atgggaccgc cctactgagc tgaggtggtt cggctggcgg ccacgacccc gcaccaggtg
     1981 gtaggcacga gccgttttcg ggatccgcgt tggcgcaatt cccggcgcca ggttcacctg
     2041 ctcctgtcgt cctctcccgc accctcccac cacccggcgt cgagcgccca gacctcctcg
     2101 acccgcgcca cccgccgcag cc
//
LOCUS       DASUBI010000105         6476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_775456, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000105 DASUBI010000000
VERSION     DASUBI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775456"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFU72_02015"
     CDS             <1..669
                     /locus_tag="VFU72_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422289.1"
                     /translation="RDLQSHEKHAGLPLTRAIAEGFAWLWREPLLRFLTLINGGDSLR
                     YGAGYLVIIVLAEELDASPRGIGAIFTGAAIGALLGNLASGRVRGRVRFGTLAISMLW
                     LEALMFPLYAVAPGVFFMGLIAAAEEFVGPMYNVSFSSYRLMATPDAMRGRTSSTVQM
                     VTQGAQSLGAILGGVLIQAAGAHWSALLLGGWLMLLALATTLNRRVRAASLEVPQATG
                     TLVP"
     gene            738..1592
                     /locus_tag="VFU72_02020"
     CDS             738..1592
                     /locus_tag="VFU72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422290.1"
                     /translation="MGKPRVDELSEAVRVLREWQYDGAPMQLHPGDLGWFWRFGAEAT
                     AGAVRTWSRNGQILAVGLLDGPALLRLAIAPGAQQDEELARQMVDDVAEPERGVLPAG
                     KVSVEAPLDALIQDLLFEAGWNTDEPWTPLRRDLREPVADPGMRIEVIGPERAQVRAA
                     VQRASFDRSTFTDERWHSMAAGLPYADARCLVAYDEQDNAVAAVTVWSAGPGKPGLLE
                     PMGVHREHRGHGFGRAITVAAAAALRDLGSSSAIVCTPSANVGAVATYQSAGFRARPK
                     IRDRYRGV"
     gene            complement(1774..1998)
                     /locus_tag="VFU72_02025"
     CDS             complement(1774..1998)
                     /locus_tag="VFU72_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU5422291.1"
                     /translation="MATAILKVPDISCEHCERTVKNALGQVAGVKQVSVDIPDKEVTV
                     DYDDRQVGVDTLREALAEEDYPVAAVVETK"
     gene            complement(2051..4846)
                     /locus_tag="VFU72_02030"
     CDS             complement(2051..4846)
                     /locus_tag="VFU72_02030"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5422292.1"
                     /translation="MVTETKETGGATREVTFPVSGMTCASCVRRVEKALSRVTGVERA
                     GVNLATEKATVDYDPLTVDLDQLKQAVEKAGYGAGPFPTEAPAPTAAPAHEIAAELTL
                     PIEGMTCASCVRRVEKALSKVEGVEQASVNLATEQARVVFDPLRVRLPQLEAAVAKAG
                     YQIGQIAAVETPAQALGAPEPPLAGEGEESLGEHERQRLREIDDLKRKWQFSLATGVV
                     MMALMYLPLGISMTTLAPLLLIAATVVQVWAGAGFYRAAWAAARHGSTNMNTLVAVGT
                     SVAYGYSAFVTLWPDLAMRWGFPDHLYYETAVIIVALILLGRWLEARAKRQTGAAIKA
                     LMGLQARTARVIRDGVEQDIPVERVQAGDLVRVRPGEKVPVDGEIVEGRSTLDESMLT
                     GESLPVEKAPGDTVIGATINRSGSFVMRATRVGRDTTLAQIVRLVEEAQGSKAPMQRL
                     ADTISSYFVPAILALAALTFAGWLLVGPGLTAALTAAIAVLIIACPCALGLATPTAIM
                     VGTGKAAEYGILVRGGEALEMTRRVDTIVLDKTGTLTRGKPAVTRIATLGDRDETDLL
                     RLAAAAEVGSEHPLGEAIVARARELGLALPAAGSFEAITGRGVVAEVEGHALLIGNRA
                     LLDDHGIALGGLPATAEELAQTGATPIYLALDGAPAAVLAVADTLKPEAPAAVAELGA
                     LGFEVWMLTGDNAATAQAIAAQAGIERVMAEVLPGEKAAQVRALQARGKTVAMVGDGI
                     NDAPALAQADLGIAIGTGTDVAMAASDITLIGGDLRTIVTAIALSRRTVSTIKQGLFW
                     AFGYNVILVPVAIGALYPFFGVLLSPILAAAAMAMSSVSVVSNALRLRRFRPPRDAAE
                     ILHPPLSRRIGEYAYLVVIALLALAIGALALYLAPDQAGLGAAGAIDPGAHQAAVAQL
                     DHALRLVAGMKGVSTVI"
     gene            complement(4881..5207)
                     /locus_tag="VFU72_02035"
     CDS             complement(4881..5207)
                     /locus_tag="VFU72_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU5422293.1"
                     /translation="MSATTEQCEHQHQHQHDQPHYSYEQDALLKRLRRIEGQVRGIQR
                     MVEEGRYCIDIVTQLQAVQAAADKVAQEVLEQHIRGCVTNAIREENGDAAIEELMNVL
                     GKAMRR"
     gene            complement(5452..>6476)
                     /locus_tag="VFU72_02040"
     CDS             complement(5452..>6476)
                     /locus_tag="VFU72_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422294.1"
                     /translation="GIIHPYYTVALAPAIGALAGIGAAELWQRRDDLLARLLLAAMLA
                     ASAVWAYELLDRTPDWYPLLRDGVLALGLGSALVLLAAGMLRRQAAAAVAVAALLASL
                     AAPAAYAAQTASTPHSGAIPSAGPAPARGRANAGRGGFPGNFGGAVPRTGSFGGNGQG
                     TGQAQGRAQGQGSGTTGGSRGGGNTRSQVPRGFAGQFGPGGIRGGLGGLLDANTPSPA
                     LVDLLRQGSSGYTWAAAAVGANDAAGVQLALDLPVMAIGGFNGTDPAPTLAQFQQYVR
                     AGKVHYFIASGGFAGGLAGFGRPGGSGSGGNTAGQISRWVAQNFAASTVGGVTVYDLT
                     QSTVAP"
BASE COUNT          880 a         2282 c         2346 g          968 t
ORIGIN      
        1 cgcgacttgc agagtcatga gaagcacgcc ggtttgccgc tgacgcgtgc catcgccgag
       61 ggcttcgcct ggctctggcg cgagccgctg ttgcgcttcc tgacgctgat caatggcggc
      121 gacagcctgc gctacggcgc cggctacctg gtcatcatcg tgctggccga ggagttggac
      181 gcctcgccgc gcggcatcgg cgcgatcttc accggggctg ccatcggcgc gttgctcggc
      241 aacctggcca gcgggcgggt gcgcggccgg gtgcgcttcg gcacgctcgc gatcagcatg
      301 ctctggctcg aagccctgat gttcccgctc tacgcggtcg cgccgggcgt gttcttcatg
      361 gggctgatcg cggcggcgga ggagttcgtc gggccgatgt acaacgtctc gttcagcagc
      421 taccggctga tggccacccc cgacgcgatg cgtgggcgca ccagcagcac ggtgcagatg
      481 gtgacgcagg gggcgcagtc gctgggggcg atcctcgggg gcgtgttgat ccaggcggcc
      541 ggcgcccact ggagcgctct gctgctcggc ggctggctga tgctgctggc gttggccacc
      601 acgctcaacc gccgcgtgcg cgccgcctcg ctcgaggtcc cacaggcgac cggaaccctc
      661 gttccctagg acgaggtgat ctcgtggcct tggggcccgc gcggagagcg gcgtatcata
      721 gagctatggc gatcgtattg ggcaagccga gagtcgacga gttgagcgag gcggtgcgcg
      781 tgctgcggga gtggcagtac gacggtgcgc cgatgcaact gcacccgggg gacctcggct
      841 ggttctggcg gttcggcgcg gaggcgacgg ccggggcggt ccggacctgg agccggaacg
      901 gacagatact cgccgtcggg ctgctggacg gtcccgcgct gctgcggctg gcgatcgcgc
      961 caggcgccca gcaagacgag gagctggcgc gacagatggt cgacgacgtg gccgagccgg
     1021 agcgcggcgt actgccagcg gggaaggtgt ccgtcgaagc gccgctggac gctttgatcc
     1081 aagacctgct gttcgaggcc ggctggaaca ccgatgagcc gtggacgccg ctgcgccgcg
     1141 acctgaggga gccggtggcg gaccctggca tgcgcatcga ggtgatcggc ccggagcggg
     1201 cgcaggtgcg ggccgccgtg cagcgagcat ccttcgaccg gtcgacgttt acggacgagc
     1261 gctggcactc catggcggcc ggcttgccct acgccgacgc ccgctgcctg gtcgcctacg
     1321 acgagcagga caacgcggtg gcggcggtga cggtgtggtc ggccggcccg gggaagcccg
     1381 ggctgctcga gccgatgggc gtgcaccggg agcatcgcgg tcacggcttc ggccgggcga
     1441 tcacggtggc agcggcagcc gcactgcggg atttgggctc gtcgagcgcg atcgtctgca
     1501 ccccgagcgc caatgtcggg gccgtcgcca cctaccagtc ggccggcttc cgggcacgcc
     1561 ccaagatccg agaccgctac cggggcgtct gagtcgggtt ggccgagtcg gtcttgattc
     1621 agatccgcgt ggcggagagg ccgatcgcgg cgccgcaccg ctgcttgagc tgtaagaccg
     1681 cgccgagcag aacccaaagc catttgagga atacgcccgg cccctctccc aatactgcga
     1741 gaggggccgg gctccgtgcg ggatgcgagc gacctacttc gtctccacca cggccgcgac
     1801 tgggtagtcc tcctcggcca gagcctcgcg cagcgtgtcg acgccgacct gccggtcgtc
     1861 gtagtcgacg gtcacttcct tgtcggggat gtcgacactg acctgcttga cgccggctac
     1921 ctggccgagc gcgttcttaa ccgtgcgctc gcagtgctcg caggagatgt cgggtacctt
     1981 cagaatcgcc gttgccatgc tcgtttcctt cctgacacca tcagccaatc gctcgtccgc
     2041 acgccggcag ctagatcacc gtgctgaccc ctttcatgcc cgccacgagc cgcagggcgt
     2101 ggtcgagctg ggccacggcc gcctggtgcg cgccgggatc gatcgcgccc gcggcgccca
     2161 gccctgcctg gtccggcgcc aggtacagcg ccagcgcgcc gatcgccagc gcgagcagcg
     2221 cgatcacgac cagataggcg tactcgccga tgcgccggga gagcggcggg tgcaggatct
     2281 cggcggcatc gcgcggcggg cggaagcggc gcagccgcag cgcgttgctg accacgctga
     2341 cgctgctcat cgccatggcc gccgcggcca ggatcggact gagcagcacg ccgaagaacg
     2401 ggtagagcgc gccgatcgcc accggcacca ggatcacgtt gtagccgaag gcccagaaga
     2461 gcccctgctt gatcgtgctg accgtgcggc gcgagagcgc gatggcggtg acgatggtgc
     2521 gcaggtcgcc gccgatgagc gtgatgtcgg aggcggccat ggccacgtcg gtgccggtgc
     2581 cgatggcgat gccgagatcg gcctgggcca gcgccggggc gtcgttgatg ccgtcgccca
     2641 ccatcgcgac cgtcttgccg cgtgcctgca gcgcgcgcac ctgcgccgcc ttctcgccgg
     2701 gtagcacctc ggccatgacg cgctcgatgc cggcctgcgc ggcgatcgcc tgggcggtgg
     2761 cggcgttgtc gccggtcagc atccagactt cgaaaccgag cgcccccagc tcggccaccg
     2821 ccgcgggcgc ctcgggcttg agcgtgtcgg ccaccgcgag caccgcggcc ggcgcgccgt
     2881 cgagcgccag gtagatcggc gtcgcgccgg tctgggccag ctcctcggcc gtggccggca
     2941 gcccacccag cgctatacca tggtcgtcca gcagcgcgcg gttgccgatc aggagcgcgt
     3001 gtccctcgac ctcggccacc acgccgcgcc cggtgatggc ctcgaacgag ccagccgccg
     3061 gcagcgccag ccccagctcg cgggcgcggg ccacgatcgc ctcgccgagc gggtgctcgg
     3121 agccgacctc ggcggccgcc gccagccgca gcaggtcggt ctcgtcgcgg tcgcccagcg
     3181 tcgcgatgcg ggtaaccgcc ggcttgccgc gcgtcagcgt gccggtcttg tcgagcacga
     3241 tggtatcgac ccggcgcgtc atctccagcg cctcgccgcc gcgcacgagg atcccgtact
     3301 cggccgcctt gccggtgccg accatgatcg cggtcggggt ggccaggccg agggcgcagg
     3361 ggcaggcgat gatcagcacc gcgatggcgg cggtcagcgc ggcggtcagg ccggggccga
     3421 cgaggagcca gccggcaaac gtcagcgccg ccagggccag gatcgccggc acgaagtagc
     3481 tggagatggt atcggccagc cgctgcatcg gcgccttgga gccctgcgcc tcctccacca
     3541 gccgcacgat ctgggcgagc gtggtgtcgc gcccgacccg cgtggcgcgc atgacgaagc
     3601 tgccgctgcg attgatggtg gcgccgatga ccgtgtcgcc gggcgccttc tcgaccggca
     3661 ggctctcgcc ggtcagcatg ctctcatcga gcgtcgagcg accctcgacg atctcgccgt
     3721 cgaccggcac cttctcgccg ggacgcacgc gtaccagatc gccggcctgc acgcgctcga
     3781 ccgggatgtc ctgctcgacg ccgtcgcgga tgacgcgcgc ggtacgcgcc tgcagaccca
     3841 tcagcgcctt gatcgccgcg ccggtctgcc gcttggcgcg cgcctccagc cagcggccga
     3901 gcaggatcaa cgcgacgatg atgacggccg tctcgtagta aaggtgatcg gggaagcccc
     3961 agcgcatcgc cagatcaggc cagagcgtga cgaaggcgct gtagccgtag gcgacgctgg
     4021 tgccgaccgc gaccagcgtg ttcatgttgg tgctgccgtg gcgggcggca gcccaggcgg
     4081 cgcggtagaa gccggcgccg gcccagacct gcaccacggt cgcggcgatc agcagcagcg
     4141 gcgccagcgt cgtcatcgag atgcccagcg gcaggtacat cagcgccatc atcacgacgc
     4201 cggtggccag gctgaactgc cacttgcgct tgaggtcgtc gatctcgcgc aggcgctgtc
     4261 gctcgtgctc gccgagcgac tcctcaccct cgccggccag cggcggctcc ggcgcgccca
     4321 gcgcctgtgc gggcgtctcg acagccgcga tctggccgat ctggtagccc gccttggcca
     4381 ccgccgcttc gagctgcggc aggcggacgc gcagcgggtc gaagaccacg cgcgcctgct
     4441 cggtcgccag attgacgctg gcctgctcga cgccctccac tttggacagc gccttctcga
     4501 cccggcgcac gcaactggcg caggtcatcc cctcgatcgg cagcgtgagt tcggcggcga
     4561 tttcgtgagc gggggcagcc gtgggtgcag gcgcctcggt cggaaacggc cccgcgccgt
     4621 agcccgcctt ctcgaccgcc tgcttgagtt gatcgaggtc gacggtcagg gggtcgtagt
     4681 cgaccgtggc cttctcggtg gcgaggttga cgccggcgcg ctccacgccg gtcacgcggc
     4741 tcagcgcctt ctcgacgcgg cgcacgcaac tggcgcaggt catgccgctg accgggaaag
     4801 tcacctcccg cgtcgcgcca ccggtctcct tggtctccgt taccatgggg tctcccgtga
     4861 taggcgttgg gtcgtgccgc ctagcgccgc atggccttgc cgagaacgtt catcagctct
     4921 tcgatcgccg cgtcgccgtt ctcctcgcgg atggcgttgg tgacgcagcc gcgaatgtgc
     4981 tgctccagca cctcctgtgc gaccttgtcg gccgcggcct gcacggcctg cagctgggtc
     5041 acgatgtcga tgcagtagcg gccctcctcg accatgcgct ggatgccgcg cacctgtccc
     5101 tcaatgcgcc gcagccgctt gagcagggcg tcttgttcgt agctgtagtg cggctggtcg
     5161 tgctggtgct ggtgctggtg ctcgcattgc tcggtcgtcg cactcatgtc agatcctctc
     5221 ctcccgatac cccctcgggg tattgcgcct caatcttata ccctcggtgg gtatcgcgca
     5281 agtggtggac cgtgaggtgg gagcgtggtg gcgggcataa cgcagatcag tgggcggcga
     5341 gggcagacgt ccctacagga tggacaaggt tccacggttc tgtaggcgca gatccccgtg
     5401 tctgcccttt cgcccctacg acgaggcagc ataggcacgg gaaatgtgcc cctacggcgc
     5461 caccgtgctc tgcgtcaggt cgtagaccgt caccccgccg acggtgctgg cggcgaagtt
     5521 ctgcgccacc cagcgactga tctggccggc ggtgttgccg ccgctgccgg agccgcccgg
     5581 acggccgaag cccgcgaggc cgcccgcgaa gccgccgctc gcgatgaaat agtggacctt
     5641 gcccgcgcgc acgtactgct ggaactgcgc cagcgtcggc gccggatcgg tgccgttgaa
     5701 gccgccgatg gccatcaccg gcagatcgag cgccagctgt acgccggcgg cgtcgttcgc
     5761 gccgacggcg gcggcggccc aggtgtagcc gctgctcccc tggcgcagca ggtccaccag
     5821 tgctgggctg ggggtgttgg cgtcgagcag cccacccagc ccaccgcgga tcccgcccgg
     5881 gccgaattgg ccggcaaagc cgcgcggcac ctggctgcgc gtgttcccgc cgccgcgcga
     5941 accacctgtc gtgcccgatc cctgcccctg ggcccgcccc tgcgcctgcc ccgtcccttg
     6001 cccgttgccg ccgaaggagc cggtgcgcgg caccgcgccg ccgaagttgc cggggaagcc
     6061 gccgcgaccg gcgttggcac ggccgcgggc cggcgcgggg ccggccgagg ggatggcgcc
     6121 cgagtgcggg gtggaggcgg tctgcgcggc gtaggcggcc ggcgcggcga gggacgccag
     6181 cagcgcggcc acggcgacgg cggctgccgc ctgacgacgc agcatgcccg ccgccagcag
     6241 caccagcgcg gagccgagac cgagcgccag cacgccgtcg cgcagcagcg gataccagtc
     6301 cggggtgcgg tcgagcagct catacgccca aaccgcgctg gcggccagca tggcggccag
     6361 gaggagccgg gcgagaaggt cgtcgcggcg ctgccagagc tcggccgcgc cgatgccggc
     6421 cagcgcgccg atggcgggcg ccagcgccac ggtgtagtag gggtggatga tcccct
//
LOCUS       DASUBI010000106         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_351225, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000106 DASUBI010000000
VERSION     DASUBI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351225"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFU72_02045"
     CDS             <1..643
                     /locus_tag="VFU72_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5422295.1"
                     /translation="PDFFCAGTVARWTAGGTPVWYLVVTLGQKGVPPGVDPATFGRTR
                     EAEQQEAARLLGVEGVTFLDGVDGEVFDTLALRGQITEVIRRFRPQIVVTHDPLTRLY
                     RQHPDHRAVGFAALHAAFPASRLATFFPDQMALGLEPHPVVGALLFGSERPDTFVDIA
                     PVFERKVEALQAHVSQTSAFPGGLHDRVRSRAEEAGRQGGVALAESFLSVVLE"
     gene            697..1422
                     /locus_tag="VFU72_02050"
     CDS             697..1422
                     /locus_tag="VFU72_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5422296.1"
                     /translation="MSETVSAGEVVFLLDVDNTLLDNDQVERDLRAFLQHRFGTALAE
                     RFWAIFEALRAELGYADFLGALQRFRLEHLRHPHILETTFYLLDYPFAERLYPGALDV
                     VAHLGALGTPVILSDGDVVFQPYKIRASGIWQAVERNVLIYIHKEAMLEDIAARYPAR
                     HYVLVDDKVRILAAIKAVWGARVTTVFPRQGHYAHDAALVARYPTPDLTIARIGDLLN
                     LRLDALVEAALPSASAKAANGER"
     gene            complement(1446..1730)
                     /locus_tag="VFU72_02055"
     CDS             complement(1446..1730)
                     /locus_tag="VFU72_02055"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEU5422297.1"
                     /translation="MLFMVIERFKDDDMLPIYRRLRDRGRMLPEGLTYVASWIEPSFA
                     RCFQLMECDDARLLQEWVLGWRGTGATFEIVPVVPSAETRTVVAPLLDEV"
     gene            1875..>2948
                     /locus_tag="VFU72_02060"
     CDS             1875..>2948
                     /locus_tag="VFU72_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5422298.1"
                     /translation="MSVDESRQRRQQLIEQLRASGALRDEAVAGAFRAVPREIFLPGL
                     PLDQVYQDQAIVTKEENGVGVSSSSQPAIMAIMLEQLDVRPGMQVLEIGAGTGYNAAL
                     LQELVGESGRVVTVDIDPEIAGWARERLRAAGYPRVAVVCADGAEGYAPVAPYDRVQL
                     TVGSAEIAPAWAEQLSPGGRLVLPLWVNTAQLSVCFERCDDHLVSRSLQPCGFMAIRG
                     ALAGPNRYAPLANDLMVMPGRSALPLDELAGLLRRPGGSQPVPLGLTPGWGFTLWLAL
                     RGDRVLGVISHESAVGFSGAGFGLYLGGAEPGIALLVLPADESRPADLVWFGAPRARD
                     VLLRELAAWRAAGEPVTGDFELAA"
BASE COUNT          386 a         1109 c         1012 g          441 t
ORIGIN      
        1 tcccgacttc ttctgcgccg gcacggtggc gcgctggacg gccggcggca cgccggtctg
       61 gtatctggtg gtaaccctgg gccagaaggg tgtgccgccg ggcgtcgacc ccgccacctt
      121 cggccgcacg cgcgaggcgg agcagcagga ggccgcgcgg ctgctcggcg tcgagggcgt
      181 cacctttctc gacggcgtcg acggcgaggt cttcgacacg ctggcgctgc gcggccagat
      241 caccgaggtc atccgccgct tccgaccgca gatcgtcgtg acgcacgacc cgctgacgcg
      301 cctctaccgc cagcaccccg accaccgcgc ggtcggcttc gccgcgctgc acgccgcctt
      361 cccggcctcg cgcctggcca ccttcttccc cgaccagatg gcgctcgggc tggaaccgca
      421 cccggtcgtg ggcgcgctcc tcttcggcag cgagcggccc gacacgttcg tcgatatcgc
      481 gccggtcttc gagcgcaagg tggaggcgct ccaggcgcac gtctcgcaga cctccgcctt
      541 cccgggcggg ctgcacgacc gcgtgcgcag ccgggccgag gaggccgggc gccagggcgg
      601 ggtggcgctg gccgagtcgt tcctctcggt cgtgctggaa tagcgtctgc aagcggtcgt
      661 tccggggcga gccggtcgcc gcgcgagagg agcgccatgt ccgagacagt atcagccggc
      721 gaggtcgtct tcctgctcga cgtcgacaac acgctgctcg acaacgacca ggtcgagcgc
      781 gatctgcgcg cctttctgca gcaccgcttc ggcacggcgc tggcggagcg cttctgggcc
      841 atcttcgagg cgctgcgggc cgagttgggc tacgccgact tcctcggcgc gctgcagcgc
      901 ttccggctgg agcacctgcg ccacccacac atcctggaga ccaccttcta cctgctcgac
      961 taccccttcg ccgagcgcct ctaccccggc gcgctggacg tcgtcgccca cctgggcgcg
     1021 ctgggcacgc cggtcatcct ctccgacggc gacgtcgtct tccagcccta taagatccgc
     1081 gcgtcgggca tttggcaggc ggtcgagcgc aacgtgctga tctacatcca caaggaagcc
     1141 atgctggagg acatcgccgc gcgctacccg gcacggcact acgtgctggt cgacgacaaa
     1201 gtgcgcatcc tggcggcgat caaggccgtc tggggcgcac gcgtcaccac cgtcttccct
     1261 cgtcaggggc actacgccca cgacgccgca ctggtcgccc gctaccccac gccggacctg
     1321 accatcgcgc gcatcggcga cctgctcaac ctccggctcg acgcgctggt ggaagccgcg
     1381 ctgccgagcg ccagcgccaa ggcagcgaac ggggagcggt aatccgcttc ggcgtcttgc
     1441 agccctcaca cctcgtcgag cagcggcgcc accaccgtgc gcgtctcggc gctgggcacg
     1501 accggcacga tctcgaaggt ggcgcccgtg ccgcgccagc cgagcaccca ctcctgcagg
     1561 agccgggcgt cgtcgcactc catcagttgg aagcagcgcg cgaagctggg ctcgatccag
     1621 ctagcgacgt aggtcagtcc ctcggggagc atgcgtcccc ggtcgcgcag ccgccggtag
     1681 atcggcagca tgtcgtcgtc cttgaagcgc tcgatcacca tgaagagcat acggtctccc
     1741 cttccccttc ccctgcaccg gtccgcccgg cgtcgcgccg ccaagactac cgtgaaggat
     1801 cgccaggcga cagccgccct ttgccgatcc acgccgggct gctaccgtag ccgggagcga
     1861 ggggaggcgg ccgggtgagt gtcgacgagt cgcggcagcg gcgacagcag ttgatcgagc
     1921 aattgcgcgc ctcgggcgcg ctgcgcgacg aggccgtggc cggcgccttt cgcgccgtgc
     1981 cgcgcgagat cttcctgccc ggcctgccgc tcgatcaggt ctatcaggac caggcgatcg
     2041 tgaccaaaga agagaacggg gtgggcgtca gctcctcctc gcagccggcg atcatggcga
     2101 tcatgctgga gcagctcgac gtgcgccccg gcatgcaggt gctggagatc ggcgccggca
     2161 ccggctacaa cgccgccctc ctgcaagagc tcgtcggcga gagcggacgc gtcgtcacgg
     2221 tcgatatcga ccccgagatc gctggttggg cgcgcgagcg gctgcgcgcc gccggctacc
     2281 cgcgggtcgc ggtcgtctgc gccgacggcg ccgagggcta cgcgcccgtc gcgccctacg
     2341 accgcgtcca gctcaccgtc ggcagcgccg agatcgcccc cgcctgggcc gagcagctct
     2401 cccccggcgg gcggctggtg ctgccgctct gggtcaacac cgcccagctc agtgtctgct
     2461 tcgagaggtg cgacgatcat ctggtcagcc gctcgctcca gccctgcggc ttcatggcga
     2521 tccgcggcgc gctcgccggt cccaaccgct acgccccgct cgcgaacgac ctgatggtga
     2581 tgcccggccg ctccgcgctc ccactcgacg agttggcggg tctgctgcgc cggcccggcg
     2641 gcagccagcc ggtgccgctg gggctcaccc ccggctgggg tttcaccctc tggctggcgc
     2701 tgcgcggcga ccgcgtgctg ggcgtcatct cgcacgagag cgccgtcggc ttcagcggcg
     2761 ccggcttcgg gctctacctc ggcggcgccg agccgggcat cgcgctgctg gtcctgcccg
     2821 ccgacgagag ccggccagcc gatctcgtct ggttcggcgc gccgcgggcc cgcgacgtgc
     2881 tgctgcgcga gctggcggcc tggcgggcgg cgggcgagcc ggtgaccggc gatttcgagc
     2941 tggccgcc
//
LOCUS       DASUBI010000107         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_432919, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000107 DASUBI010000000
VERSION     DASUBI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432919"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFU72_02065"
     CDS             complement(<1..739)
                     /locus_tag="VFU72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422299.1"
                     /translation="MTQETLAERASLSVRAISDLERGLKATPRRETVELLAAALGLPV
                     EELERAVARRRGARSGRSPAQLPAAPTPLVGREAEVRALTRLLRQDDTRLVTVTGPPG
                     IGKTRLALAVAEAVAGGFPGGVVFVPLAAQRDPAQVPLNLAQALGVPRDGPGSLTERL
                     AEELRGRQVLVLLDNFEQVLEAAPTLAELLAACPRLKLLVTSRAALQLQSEHRCALAP
                     LGVPAPGQLPALDDLASVPAVVLFERRA"
     gene            963..>3760
                     /locus_tag="VFU72_02070"
     CDS             963..>3760
                     /locus_tag="VFU72_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422300.1"
                     /translation="MSRYLSVILAVAVVASGLTLLLTPATAGAAAQQVTMRVTIKEVR
                     GISCFEGTVPVIGGCAGLPDFYAAVIMPIGPEKFSPIIDDTNQIFPNWTFEATLDYIP
                     GVSHPFHIEIWEDDGGLRLGDDQADINGNPGDKADLDLTLTLGSVPCQISGDATGTCG
                     IDITTQGNGDADGDARLTFRVDVVNNLPDGDGDGIPDDWERNGVTLNGQLIDLPAMGA
                     DPNRPDIFLHVDWMADATHDQRLDNAAIQNVVNAFDNSPYRSPTGSTGINLHVDQGQN
                     STLNFASGATWGALSRARDIPWQNNLGATTNGQYDWSQFQAIKSANFDPTGRSPIFHY
                     VIAAFLLQPPTPGGAQDTSSGISRGIGTSDFIISFGGTGGPGVPQQQAGTLMHELGHN
                     LGLRHGGNENTNYKPNYLSIMNYLYTLRGLDAGASFGIVDFTGGRFPVLGPLNEGTLD
                     EAKALGLAGFGTGSRCPVAGGFTEQYTTNANGPFDWNCNGKTTDGVVSVDANGSGGLD
                     GSLTSFDDWANIKLREGAIGDLGVAPLPMLSDPEPPLAWDYTPPSTTVSVTPAPTNYG
                     WNKAAVTVTLRATDNPGGFGIHHLNYSATGAQSLAGTNVFSDTASFVIANEGITTISY
                     SAYDHALNQEATKTLVVRVDLTDPTVALGTAYPAPNAAGWNNTDVNVPFTATDTPSGI
                     ASTAPPASPLVLTDEGAAVTGTVAAVDKADRLTTVTTPAFKIDKTPPVITVTSPGENQ
                     VFDSDQTMTPVFSATDALSGVKSVVAVLDTGQELTSGTAIELGLLVGKRTLTVTATDV
                     ADNVASVEIHFRVRPVFVGNAFTDANQSAVREAAEAGLSGVTVFLDTNGNGRLDAGEA
                     SNVTAAGGAYRVVGEDVGPARVCTLPLAGDRVRTTSRCQTVMVATGIPVPHTDFGSHT
                     RIAGASSGSVANVIQSVA"
BASE COUNT          637 a         1393 c         1230 g          500 t
ORIGIN      
        1 gcgcccgccg ctcgaagagg acgacggcgg gaacggacgc cagatcgtcg agcgcgggga
       61 gttgccccgg cgccgggacg ccgagcggcg ccagcgcgca gcggtgctcg ctctggagct
      121 ggagcgcggc gcggctggtg accagcagct tcagccgcgg gcaggcggcc agcagctcgg
      181 caagcgtggg cgccgcctca agcacctgct cgaagttgtc gagcaggacg agcacctgcc
      241 gaccgcgcag ctcttccgcc aatcgctcgg tcaggctgcc ggggccatca cgcggcacgc
      301 cgagcgcctg ggccaggttg agcggcacct gcgccgggtc ccgctgcgcc gccagcggca
      361 cgaagacgac gccgccgggg aagccgccgg ccacggcctc ggccaccgcc agcgccagcc
      421 gggtcttgcc gatcccgggc gggccggtca ccgtcaccag gcgcgtgtcg tcctgccgca
      481 acagccgcgt gagcgcgcgc acctccgcct cgcggccgac gagcggcgtc ggcgcggcgg
      541 gcaactgggc cggcgaccgg ccggagcgcg cgccgcgccg ccgcgccacg gctcgctcca
      601 gctcctcgac cggcaggccc agcgcggcgg ccagcaactc gaccgtctca cggcgcggcg
      661 tcgctttcag gccgcgctcc aggtcgctga tggcgcgcac gctcaggctc gcccgctcgg
      721 ccagcgtttc ctgggtcaat ccggctgcca gccgatggtg gcgaagtaat cgagcaaaag
      781 gggtcatggc gcccgtcagt gtcgcgcatg gcgagcaaac cgcacgcgct aaagatatca
      841 cccaccgcat acgtttccca acgtgtgggt tggtcgtgtg ggttgtgtct ggcctcgctc
      901 ccgcgccctg agcagagtta ggagaacggc gcgacgcccg gtattgaggg aggagagagg
      961 tcgtgtctcg ctatctcagc gttatcctgg ccgtggcggt ggtcgcctcg ggtctcacgc
     1021 tgctcttgac cccggccacc gctggcgcgg cggcgcagca ggtcaccatg cgggtgacga
     1081 tcaaggaggt tcggggaatt tcgtgcttcg agggaaccgt cccggtcatc ggcggctgcg
     1141 cgggcctgcc tgacttctac gcggcggtga tcatgccgat cggccccgag aagttcagcc
     1201 cgattatcga cgacaccaac cagatcttcc ccaactggac gttcgaggcg acgctcgact
     1261 acatccccgg cgtgagccac cctttccaca tcgagatctg ggaagacgat ggcggtctgc
     1321 gcctcggcga tgaccaggcc gacatcaacg gcaaccccgg cgacaaggcc gacctcgatc
     1381 tgacgctgac gctcgggagc gtgccctgcc agatcagcgg cgacgcgacc ggcacctgcg
     1441 gcatcgacat caccacccag gggaacggcg acgccgacgg cgacgcgcgt ctcaccttcc
     1501 gggtcgatgt cgtcaacaac ctgccggacg gcgacggcga cggcatcccg gacgattggg
     1561 agcgcaacgg cgtcacgctg aacggccagc tcatcgacct gccggcgatg ggcgccgacc
     1621 ccaaccgacc cgacatcttc ctgcatgtcg actggatggc ggacgcgacc cacgaccagc
     1681 ggctggacaa cgccgccatc cagaacgtgg tgaacgcctt cgacaactcg ccctaccgca
     1741 gcccaaccgg ctcgaccggc atcaacctgc acgtagacca ggggcagaac agcacgctca
     1801 acttcgccag cggcgcgacc tggggcgcgt tgagccgtgc ccgggacatc ccctggcaga
     1861 acaacctcgg cgccacgacc aacgggcagt atgactggtc gcagttccag gccatcaagt
     1921 cggccaactt cgacccgacc ggccgctcgc cgatcttcca ctatgtgatc gcggccttcc
     1981 tgctgcagcc gccgacgcca ggcggcgccc aggacacctc cagcggcatc tcgcgcggca
     2041 tcggcaccag cgacttcatc atctcgttcg gcggcaccgg cggccccggc gtgccgcagc
     2101 agcaggccgg cacgctgatg cacgagctgg ggcacaacct cggcctgcgg cacggcggca
     2161 acgagaacac caactacaag ccgaactacc tcagcatcat gaactacctc tataccctcc
     2221 ggggactcga tgccggggcg agcttcggca tcgtcgattt caccggcggg cgattcccgg
     2281 tgctcggccc gctcaacgaa ggcacgctgg atgaggcgaa ggcgctgggc ctggccggct
     2341 tcggcaccgg ctcgcgctgc ccggtggccg gcggcttcac cgagcagtac acgaccaacg
     2401 ccaatggccc cttcgactgg aactgcaacg ggaagacgac cgacggggtg gtcagcgtcg
     2461 atgccaacgg cagcggcggc ctcgatggca gcctgaccag cttcgacgac tgggccaaca
     2521 tcaagctccg cgagggcgcg atcggcgacc tcggcgtcgc gccgctgccg atgctgagcg
     2581 accccgagcc gccgctcgcc tgggactaca cgccacccag cactacggtg agcgtcacgc
     2641 ccgcgccgac caactacggc tggaacaagg cggccgtcac cgtcaccctg cgcgcgaccg
     2701 acaaccctgg cggcttcggc atccaccacc tcaactacag cgcgaccggc gcgcagtcgc
     2761 tcgccggcac gaatgtcttc agcgacacgg ccagcttcgt gatcgccaac gagggcatta
     2821 ccaccatcag ctactccgcc tacgaccatg cgctcaacca ggaggcgacc aagacgctgg
     2881 tagtgcgcgt cgacctgacc gatccgacgg tcgcgctcgg cacggcctac ccggcgccca
     2941 acgcggccgg ctggaacaac accgacgtca acgtcccctt caccgcgacg gatacgccct
     3001 cgggcatcgc gtccaccgcc ccgccggcca gcccgctggt gctgaccgac gagggtgcgg
     3061 cggtgaccgg cacggttgcg gccgtggaca aggccgaccg gctgaccacc gtcacgactc
     3121 ccgcattcaa gatcgacaag actccgccgg tgatcaccgt cacctcgccg ggcgagaatc
     3181 aggtcttcga ctccgaccag acgatgacgc cggtcttcag cgctaccgac gcgctctcgg
     3241 gggtcaagag cgtggtcgcg gtcctcgaca ccggccagga gctgaccagc ggcaccgcca
     3301 tcgagctggg cctgctggtc ggcaagcgca ccctgaccgt caccgccacc gacgtggccg
     3361 acaacgtcgc cagcgtcgag atccacttcc gcgtccggcc agtcttcgtc ggcaacgcct
     3421 tcaccgacgc caaccagagc gccgtgcgcg aggcggcgga ggccgggctg agcggcgtca
     3481 cggtcttcct cgataccaac ggcaacggcc ggcttgacgc cggtgaggcc agcaacgtca
     3541 ccgcggccgg cggcgcctac cgcgtggtcg gcgaggatgt cggcccggcc cgcgtctgca
     3601 ccctgccgct ggccggcgac cgggtgcgca ccaccagccg ctgccagacg gtcatggtgg
     3661 ccaccggaat ccccgtgccg cataccgact tcggatccca tactcggata gccggagcga
     3721 gtagcggtag tgtggccaac gttatccagt ccgtggcgca
//
LOCUS       DASUBI010000108         5678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_733012, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000108 DASUBI010000000
VERSION     DASUBI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733012"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..859)
                     /locus_tag="VFU72_02075"
     CDS             complement(95..859)
                     /locus_tag="VFU72_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422301.1"
                     /translation="MSTFAVLLGKDLTEQWRTRRLLIVAIIFLLLGLGSPLLAYYTPQ
                     LVERFGGDIQVTIPTPTAADAVEQLLKNLSQFGPFAAILLAMGAVARELERGTAAFIL
                     SKPVGRDAFLASKFLALALTLAVAVLVAAVGAYFYTAVLFSRLPLAGFVAACLLMLLL
                     LLAYAAFTFLGSALLGSALPAAGLGFALWVLSGIVGVIPHAGRFTPLGLTDPARGLAL
                     GQPATHLGASLAATLVLIALTLAATWLVFRSKEIES"
     gene            complement(856..1827)
                     /locus_tag="VFU72_02080"
     CDS             complement(856..1827)
                     /locus_tag="VFU72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422302.1"
                     /translation="MGVPEPAIACQQLVKRYGAVRALDGLDLSVPSGSIFGFLGPNGA
                     GKTTTLRILTGLAEPTSGRALIAGHDVGRRGAALRREIGYLEQQPQFYGWMRARELLA
                     FAGELFGLRGAPLRARVDEVLEQTGLQAAAGRRVGGFSGGMRQRLGLAQAMINRPPVL
                     LLDEPVSQLDPAGRHDLLRIIAGLRGQATVLMSTHILADVERVCDRVAIIDRGRLLVE
                     APVAELERRYTQPVFVLEPEAGQDERVDALLAALREQPWVASVTREGAAVRVTTSEPE
                     VAGRAILPLVVAAGVTLARFERGRASLEDIFLRLVGDERTHPAEAAS"
     gene            complement(1827..2024)
                     /locus_tag="VFU72_02085"
     CDS             complement(1827..2024)
                     /locus_tag="VFU72_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5422303.1"
                     /translation="MSSQQTILLLLPIVLISLGLLLYGLYDLTRPWRRVKGGNKWVWG
                     VILFLNIIGPLAYLLAGREEE"
     gene            complement(2044..2379)
                     /locus_tag="VFU72_02090"
     CDS             complement(2044..2379)
                     /locus_tag="VFU72_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU5422304.1"
                     /translation="MQSLMVVTTERIPGYEVVQVHGAVYGVVVRSRGLGGDIMASLRS
                     VVGGEIHEYTQMLEQARQHALDRMIEHATSLGANAVVMARFDSSEIGKTMSEIVAYGT
                     AVTIRRQLA"
     gene            2536..3273
                     /locus_tag="VFU72_02095"
     CDS             2536..3273
                     /locus_tag="VFU72_02095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422305.1"
                     /translation="MSETAPGLPGWGEDDSAFFLRWADVVIPRRAEQLALLVALAPFT
                     AQAEFTAVDLACGAGELSRRLLERFPRCHVLALDGSPAMLNAAAANLAPFGARAETGR
                     FDLFADDWRAQLPHDLGCVVSSMALHHLPGPEKRRLYRDLAAHLAPGGALLVQDIVEP
                     ASAEARQYYADRWDSWVRARSLELTGDLAVYERFRDGWNHFATPDPEDQPSPLGAQLA
                     WLAEAGLVRADCFALCSGFAVFGAFAP"
     gene            complement(3366..4739)
                     /locus_tag="VFU72_02100"
     CDS             complement(3366..4739)
                     /locus_tag="VFU72_02100"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEU5422306.1"
                     /translation="MQYRIRELDAQVRFSEHLGLEALATVIPRDVIATVLAEQGAEAV
                     RVRKLTMVTVVLVLIAMHLYARVALGRVLSQLARGLRLVWDEPEVVLPGASALCYRRY
                     QLGAQPLVALFHRICQPLTTPATPGAYRFGLRLLALDGTVEDVADSPANVRAFGRHHS
                     RRGDSAFPQIRVVAVVECGSHAVLDAGLWPIRVGESTVAARLLRTVGPGTLLLWDSRF
                     HSYDLLVAAQQHGAAVLGRLPAGVHPHPVRTLPDGSALVTLCPRPHTVPLPVRLVTYT
                     HNDPGRPGHGERQRLITTLLDPATAPARELILTYHERWEFETTVDELDTHLRLAGHPL
                     RSRRPVGVVQEFYALLLAHYAVRALMHAAAVQAGLDPDRLSFVAAVDLIRAAIPEFQL
                     VAPREVERLVARLLHDLARVTLPPRRNRSNPRVVKRKVAKFLRKRPEHVHWPQPTKPF
                     AEAVVLI"
     gene            complement(4905..5474)
                     /locus_tag="VFU72_02105"
     CDS             complement(4905..5474)
                     /locus_tag="VFU72_02105"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5422307.1"
                     /translation="MAIRELDLWLVGRVLEPSPLVLVTSIYHGRPNVMTAAWLTTLGN
                     DPPRVGLAIMPSRLTHEFISRSEAFALSVAPLDLLDAVHRCGTLSGRDGDKFAAAGLR
                     ELDARVIEAPLVEQGVAFIECGLVNRLGVSDHDLFIGEVLALSAAEDLFTDRWQIQEG
                     SELIHHIAGAEYTGLSKTYTVRLEPEEEG"
     gene            complement(5461..>5678)
                     /locus_tag="VFU72_02110"
     CDS             complement(5461..>5678)
                     /locus_tag="VFU72_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422308.1"
                     /translation="DSDALARLLDELAADPARRARLGEWARRRVQAEFTQASVARRYY
                     ELYRRVLGLDQTVDAGRWISGRIDGDS"
BASE COUNT          873 a         2013 c         2021 g          771 t
ORIGIN      
        1 ccgcgcgcgc tcgatggagg gctgcaattg ccgcagcgcc gcacgtcgtc gctcttgcaa
       61 ggtcgatcac tccctcgccg atgaaaacgc cgcgctagct ctcgatctcc ttacttcgaa
      121 acacgagcca ggtggcggcc agggtcaggg cgatcagcac cagggtggcg gccagcgagg
      181 cgccgaggtg cgtcgccggc tggccgagtg ccaggccgcg cgccgggtcg gtcagcccga
      241 gcggcgtgaa gcgcccggcg tgggggatta cgccgacgat accgctgagc acccacaggg
      301 cgaagccgag cccggcagcc ggcagcgccg agccgagcag cgcgctcccc aggaaggtga
      361 acgccgcata ggccagcaac agcagcagca tcaagaggca ggcggcgaca aaacccgcca
      421 gcggcagcct gctgaagagg accgccgtgt agaagtaggc gccgaccgcg gccaccagca
      481 ccgccaccgc cagcgtcagc gccagcgcca ggaactttga agccaggaag gcgtcgcggc
      541 cgaccggctt gctcaggatg aaggccgccg tgccgcgctc cagctcgcgc gccaccgcgc
      601 ccatggccag caggatggcg gcgaaggggc cgaattggct caggttcttc agcagttgtt
      661 cgaccgcgtc ggccgccgtc ggcgtcggga tggtgacctg gatgtcgccg ccgaagcgtt
      721 cgacgagctg cggcgtgtag taggccagca gcggcgagcc gagccccagc agcaggaaga
      781 tgatagccac gatcagcagc cggcgggtgc gccactgctc ggtcagatcc ttgcccagca
      841 gcacggcgaa ggtgctcacg acgccgcctc tgccgggtgc gtgcgttcat caccgaccag
      901 tcgcaggaag atatcctcca ggctggcccg cccccgctcg aagcgcgcca gcgtcacgcc
      961 cgccgccacc accagcggca ggatcgcccg gccggccacc tccggctcgc tcgtcgtcac
     1021 ccggaccgcc gcgccctcgc gggtgacgct cgccacccag ggctgctccc gcagtgccgc
     1081 cagcagcgca tcgacgcgct catcctgccc ggcctccggc tccagcacga agaccggctg
     1141 agtgtagcgc cgctccagct cggctaccgg cgcctccacc agcagccgtc cccggtcgat
     1201 gatcgccacc cggtcgcaga cgcgctcgac gtcggccagg atgtgggtcg acatcaggac
     1261 cgtcgcctgg ccgcgcagcc cggcgatgat gcgcagcaga tcgtgccggc cggcggggtc
     1321 gagctggctg accggctcgt cgagcaggag caccgggggc cggttgatca tggcctgggc
     1381 cagcccgagg cgctggcgca tgccgccgga gaagccgccg acgcgccgcc cggcggccgc
     1441 ctgcaggccg gtctgctcca gcacctcgtc gacgcgggcg cgcagcgggg cgccacgcag
     1501 gccgaagagc tcgccggcga aggccagcag ctcgcgggcg cgcatccagc cgtagaactg
     1561 cggctgctgc tctaggtagc cgatctcgcg ccgcagcgcg gcgccgcgcc ggcccacgtc
     1621 gtgcccggcg atcagcgcgc ggccgctggt cggctcggcc agcccggtca ggatgcgcag
     1681 cgtggtcgtc ttgccggcgc cgttgggccc gaggaagccg aagatcgagc cgctggggac
     1741 gctgaggtcg agcccgtcga gcgcgcgcac ggcgccgtag cgcttgacca actgctggca
     1801 ggcgatggcc ggctccggca cccccatcac tcctcctcgc gcccggccag caggtaggcc
     1861 agcgggccga tgatgttgag gaagaggatg acgccccaga cccacttgtt gccgcccttg
     1921 acgcggcgcc aggggcgagt caggtcgtag agcccgtaga ggagcagccc caggctgatc
     1981 agcacgatcg gcaggagtag aaggatcgtc tgctgcgaac tcacgccgcc ctcccgctcg
     2041 cgctcaggcc agctggcgcc ggatggtgac cgcggtgccg taagccacga tctcgctcat
     2101 cgtcttgccg atctcggagg agtcgaagcg ggccatcacc accgcgttgg cgccgaggct
     2161 cgtggcgtgc tcgatcatgc ggtcgagcgc gtgctggcgc gcctgctcca gcatctgggt
     2221 gtactcgtgg atctcgcccc cgacgaccga gcgcaggctg gccatgatgt cgccgccgag
     2281 gccgcggctg cgcaccacca cgccgtagac cgcgccgtgc acctgcacca cctcgtagcc
     2341 ggggatgcgc tcggtcgtga ccaccatcaa gctctgcaag gagtccccct ctctcgtccc
     2401 acgccccgcc gggtggcccg cgccatgcgg cggctccagc gcagacgata ctatgcgcgg
     2461 cgcgcggtgt ccgcaccagg cgccggggca gggtatgacg ggccaagcgg ccaggggcta
     2521 cgagaggagc gcgcgatgag cgagacggca ccaggcctgc ccggctgggg cgaggacgac
     2581 agcgccttct tcctgcgctg ggccgatgtc gtcatcccgc gccgcgccga gcagctggcg
     2641 ctcctggtgg cgctggcgcc cttcacggcg caggcggagt tcaccgcggt cgatctggcc
     2701 tgcggcgcgg gcgagctgag ccgccggctg ctggagcgct tcccgcgctg ccacgtgctt
     2761 gcgctcgacg gctcgccggc catgctgaac gcggccgcgg ccaacctggc gcccttcggc
     2821 gcgcgggccg aaaccggccg cttcgacctc ttcgcggacg actggcgcgc gcaattgccg
     2881 cacgatctgg gctgcgtcgt ctcgtccatg gcgctgcacc acctgcccgg cccggaaaag
     2941 cgccggctct accgcgatct ggcggcgcac ctggcgccgg gcggggcgct gctggtgcag
     3001 gacatcgtcg agccggccag cgcggaggcg cggcagtatt acgcggatcg ctgggatagc
     3061 tgggtgcggg cgcgttcgct ggagctgacc ggggacctcg cggtctacga gcgcttccgc
     3121 gacggctgga accacttcgc cacgcccgac cccgaggacc agccctcgcc cctgggcgcg
     3181 cagcttgcct ggctggcgga ggccggtctc gtgcgcgccg actgcttcgc gctctgtagc
     3241 ggcttcgccg tcttcggcgc gtttgcgccg tagaagttgg gtgggtttga ggggatttgg
     3301 ggcgtgaatc cgagacgttc gtgcgcgccg gcgcgccgtc tccgtccggc taaagccaat
     3361 accattcaaa taagcaccac cgcctccgcg aaaggcttcg tgggttgtgg ccagtggaca
     3421 tgttcggggc gtttacggag gaatttggcg accttgcgtt tgacgacgcg tggattgctg
     3481 cggttgcggc gcggggggag ggtgacacgc gccagatcgt gcagcaaccg cgcgacgagc
     3541 cgctcgacct cgcggggcgc aaccagttgg aactccggga tggcggcgcg aatcaggtcg
     3601 acggccgcga caaaactgag ccgatcggga tcgagcccgg cttggaccgc tgcggcgtgc
     3661 atcagggccc gcacggcata gtgcgccagc aacagggcgt agaactcctg gaccacgccg
     3721 accgggcgac gactgcgcaa gggatgcccg gccagccgga ggtgggtgtc aagctcatcg
     3781 acggtcgtct caaactccca gcgctcatgg taggttagga tcagttcccg cgccggggcc
     3841 gtggcgggat ccagcagggt ggtgatcagg cgctggcgct cgccgtggcc gggacggccg
     3901 ggatcgttgt gggtataggt caccaggcgg accgggaggg ggacggtgtg ggggcggggg
     3961 cacagggtca ccagggccga gccgtcgggg agggtgcgga cggggtgggg atgcacgccc
     4021 gccggcaggc ggccgagcac cgccgccccg tgctgctggg cggccaccag caagtcgtag
     4081 ctgtgaaagc gactatccca cagcagcaac gtccccgggc cgacggtccg cagcaggcgc
     4141 gcggccacgg tcgactcgcc cacccggatg ggccagaggc cggcatcgag caccgcatgg
     4201 ctgccacact cgaccacggc caccacccgg atctggggga aggcactgtc gccccggcgg
     4261 ctgtggtggc gcccgaaggc acggacgttg gccgggctat cggccacgtc ttccaccgtg
     4321 ccatcgaggg ccagcaggcg caggccaaag cggtaggcgc cgggcgtggc cggcgtggtc
     4381 agcggctggc agatccgatg aaagagcgcc accagcggct gcgcgccgag ttggtagcgg
     4441 cgataacaca gggcgctggc accgggcagg acgacctcgg gctcgtccca gaccaagcgc
     4501 aggccgcggg ccagctggct caggaccctg ccgagggcga cccgggcgta gagatgcatg
     4561 gcgatcaaga ccagcacgac ggtgaccatc gtcagcttgc gcacgcgcac ggcctcggcc
     4621 ccctgctcgg ccaacaccgt cgcgatgacg tcgcggggaa tgacggtggc cagggcctcc
     4681 aaccccaggt gctcactaaa gcggacctgg gcgtcgagtt cgcgtatcct atactgcatg
     4741 cggcggctcc ttgcgggctc ctggacggtt tgttcctacc gccagtatgc ccgctgagcc
     4801 gccgcgcggc ttatttgaat ggtattggct ttagccccgc gcgacgagag cggcgagcct
     4861 acagggaagg cgacatccgt tcaacttgcg cagccgcagc cgccctaccc ctcttcctcc
     4921 ggctccagcc gcaccgtgta ggtcttactc aggccggtgt actcggcccc cgcgatatgg
     4981 tggatcaact ccgagccttc ctggatctgc cagcggtcgg tgaacaggtc ttcggccgcc
     5041 gagagcgcca gcacctcacc gatgaagagg tcgtggtcgg agacgccgag tcgattcacc
     5101 aggccacact cgatgaaggc caccccctgc tcgaccagcg gcgcctcgat cactcgcgcg
     5161 tccagctcgc gtaagcccgc tgccgcgaat ttgtccccgt cgcggccgga gagcgtgccg
     5221 cagcggtgga ccgcgtcgag caggtcgagc ggcgccaccg agagcgcgaa ggcctcgctg
     5281 cggctgatga actcgtgggt cagccggctg ggcatgatcg ccagcccgac gcgcggcggg
     5341 tcgttgccca gcgtggtcag ccaggccgcc gtcatcacat tggggcggcc gtggtagatc
     5401 gaggtgacca gcacgagcgg gctcggctcc agcacgcgcc cgaccaacca gagatcaagc
     5461 tcacgaatcg ccatcgatcc tccccgagat ccagcggccc gcgtcgacgg tttgatccag
     5521 gcccagcacg cggcgataca gctcgtaata gcgccgcgcc acgctcgcct gggtgaactc
     5581 ggcctgcacg cgccgccgcg cccactcgcc caagcgcgcg cgccgcgccg gatcggcggc
     5641 cagctcgtcc agcaggcgcg ccagcgcgtc gctatccc
//
LOCUS       DASUBI010000109         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1359381, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000109 DASUBI010000000
VERSION     DASUBI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359381"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="cgtA"
                     /locus_tag="VFU72_02115"
     CDS             <1..1129
                     /gene="cgtA"
                     /locus_tag="VFU72_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEU5422309.1"
                     /translation="VIDDEAGVPIADLIEPGETFLVARGGKGGLGNQHFATSVHQAPR
                     LAEKGEPGQDRWLYLELKVIADVGLAGFPNAGKSTLLAASSAARPKIADYPFTTLEPN
                     LGVVQVGGPAGPTFVMADIPGLIEGAAEGIGLGHEFLRHVERTRVLIHVLDGSGGLEG
                     RDPLADFAAINAELAAYAPALAGKPQLVAINKVDLPETQTNLPRLTAALSGEGYQVFP
                     ISAATGEGVPLLLHRTMELLQELPLPVEEAPQKRRVYTLEEEDEERWEAVRRSAHHYD
                     VTGPKIERLTRMTDFANEEAAARFQRVLEASGISRALEEQGIQPGDIVHIADLELTWD
                     QEALDAEAREAAHERRRKTRRQRIAARLGQTVEDEELGQEG"
     gene            1440..>2022
                     /locus_tag="VFU72_02120"
     CDS             1440..>2022
                     /locus_tag="VFU72_02120"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEU5422310.1"
                     /translation="MAVTAGQIVAMGGGGLDPTSHLLEDFVVSLIPGQPARPRVCYVP
                     TASGDPDAGIVRFYNAFPSDAYQPSHLNLFNRRVLDLDTFLHQQDVIYVGGGNTANML
                     AIWRIHGVDRILREVLTAGVVLCGSSAGALCWFEDGVTDSYGGHAPLGDGLGFLDGNY
                     TPHWDSEPARQAIFRELTQRSGRFGYATTDCAAL"
BASE COUNT          320 a          707 c          715 g          280 t
ORIGIN      
        1 cgtgatcgat gacgaagcgg gcgtgccgat cgccgacctg atcgagcccg gcgagacctt
       61 cctggtggcg cgcggcggca agggcggtct gggcaaccag catttcgcca cctcggtgca
      121 tcaggctccg cgcctggccg agaagggcga gccgggccag gaccgctggc tctacctgga
      181 gctgaaggtg atcgccgatg tcggcctggc cggcttcccc aacgccggca agtcgacctt
      241 gctggcggcc tccagcgccg cgcgccccaa gatcgccgat taccccttca ccaccctgga
      301 gccgaacctg ggcgtggttc aggtgggcgg ccccgccggg ccgaccttcg tgatggccga
      361 cattcccggt ctgatcgagg gcgccgccga agggatcggc ctgggacatg aattcctgcg
      421 ccacgtcgag cgtacccgcg tgctgatcca cgtgctcgac ggctcgggcg ggctggaggg
      481 gcgcgacccg ctggcggatt tcgccgcgat caacgccgag ctggcggcct acgcgccggc
      541 gctggccggc aagccgcagt tggtggcgat caacaaggtc gacctgccgg agacgcagac
      601 caacctgccg cgcctgaccg cggcgctgag cggcgagggc taccaggtct tcccgatctc
      661 ggccgccacc ggcgagggcg tgccgctgct cttgcaccgt acgatggagc tgctgcagga
      721 gctgccgctg ccagtggagg aggcgccgca gaagcgccgg gtctatacgc tggaggagga
      781 ggacgaggag cgctgggagg cggtccggcg ctcggcgcac cactatgacg tgaccggccc
      841 caagatcgag cgcctgacca ggatgaccga cttcgccaac gaggaggcgg cggcgcgctt
      901 ccagcgcgtg ctggaggcct cgggcatcag ccgcgcgctg gaggagcagg gcatccaacc
      961 gggcgacatc gtgcatatcg ccgacctgga gctgacctgg gaccaggagg cgctggacgc
     1021 cgaggcgcgt gaggccgccc acgagcgccg ccgcaagacc cgccgccagc gcatagccgc
     1081 ccgcctgggg cagaccgtcg aggacgagga gctcgggcag gagggatgac gggcggcctc
     1141 ccacgtccac gctgggtggc gtgtgcggca cggatccttc gacaagctca ggatgacagg
     1201 gtaagagacg ggcggacgag gaggatttgc ctcgtccgaa aggccgcatc ctctcccccg
     1261 gtcgtccctt cggcaagctc agggcaggct ctgagcgcag gcgaaggatc cgtcctccga
     1321 ccggcaccca acgccccgga ttgccgccga gccttcccca gggcggtctt gcgacgcccg
     1381 gcccgagcgg cgatgatcca ctccagcgtg tcgcgggaca ataccggcgg gagggtgaga
     1441 tggcagtcac agcggggcag atcgtagcga tgggcggcgg agggctggat ccgacgagcc
     1501 acctgctgga agacttcgtg gtcagcctca tccccggcca gccggcgcgg ccgcgcgtct
     1561 gctacgtgcc gaccgcgagc ggcgaccccg atgccggcat cgtccgtttc tacaacgcct
     1621 tccccagcga tgcctaccag ccgtcgcacc tgaacctctt caaccggcgc gtgctcgacc
     1681 tcgacacctt cctgcaccag caggacgtga tctacgtcgg cggcggcaac accgccaaca
     1741 tgctggcgat ctggcgcatc cacggcgtcg atcgcattct tcgtgaggta ttgaccgccg
     1801 gcgtcgtgct ctgcggcagc agcgcggggg cgctctgctg gttcgaggac ggcgtgaccg
     1861 actcctacgg cgggcacgcc ccgctcggcg acgggctggg ctttctggac ggcaattaca
     1921 ccccgcactg ggacagcgag cccgcacgcc aggcgatctt ccgtgagctg acccagcgca
     1981 gcggacgctt cggctacgcc acgaccgact gcgccgcgct cc
//
LOCUS       DASUBI010000110         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_528003, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000110 DASUBI010000000
VERSION     DASUBI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528003"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFU72_02125"
     CDS             complement(<1..1140)
                     /locus_tag="VFU72_02125"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEU5422311.1"
                     /translation="MRRGIKRPALVLWALVLLLSLGITPAAAAPSGRATLAGSVPPWA
                     TSANFKSSSNTSDSVGFRVYLGWRGGDQAAALARAVSDPSSPSYGNYLTPAQFRQQFA
                     PSAGDTNSVRSWLQSQGFSVVYTPQNNHYISAEGTVAQVESAFGVQLNEYTVAGATLR
                     APDGPLSIPAALAGSVAGIIGIDQTQALTHPYNLSADAPPSAGFRNAPPCSTYWAEKI
                     ATDLPTAYGEAQPYAPCGYTPAQLQGAYGISGAIAGGNDGSGQTVAIIDAYASPTIVQ
                     DVNQYSADHGLPQMGPNQFSQVVAPGTYRRPQNPRQDPQGWYGEETLDVEAVHAMAPG
                     ANIVYVGAPNNYQDLDAALNHVVDRGLAQIVTNSYGFSTELLPPGY"
     gene            1754..2224
                     /locus_tag="VFU72_02130"
     CDS             1754..2224
                     /locus_tag="VFU72_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5422312.1"
                     /translation="MSTRPPAFGELLQRYRVRAALSQEELAERAGLSASAISALERGA
                     RQRPYPHTRQLLAQALGLSPDEAAVFTGPARGRAAVPSPSSPTTNLPARRTPLIGREE
                     ASVELRALVRQDAGRLLTLTGSGTPSSWHAPVMISRSRSKPWPISPRCGDTRAC"
     gene            2239..>2738
                     /locus_tag="VFU72_02135"
     CDS             2239..>2738
                     /locus_tag="VFU72_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422313.1"
                     /translation="MLGECLAAAQAIGDEARLAFALLSLGVTVRVQRDIARSVALLEE
                     SLERFQRLGDLRWIANAQTMLGSALVHQGESDRAAATLLAGLAGHRAVGDLTFSTSGL
                     LCLAAVLAERAQPAWAASVFGALLALNERVGTTLTTGNQREYDRIASGVRAQLGAADF
                     AWTLAEG"
BASE COUNT          374 a          900 c         1031 g          433 t
ORIGIN      
        1 gtagccgggc ggcagcagtt cggtcgagaa gccgtaggag ttggtcacga tctgggcgag
       61 accccggtcg accacgtggt tgagcgcggc gtcgagatcc tggtagttgt tcggcgcgcc
      121 gacgtagacg atgttggcgc caggcgccat cgcgtggacc gcctcgacgt cgagcgtctc
      181 ctcaccgtac cagccctgtg ggtcttggcg cggattctgg gggcgccggt aggtgccggg
      241 cgcgacgacc tggctgaact ggttgggccc catctgcggc aggccgtgat cggcggagta
      301 ctggttcacg tcctgcacga tcgtcggcga ggcgtaggcg tcgatgatcg ccaccgtctg
      361 cccggagccg tcgttgccgc cggcgatggc gccgctgatg ccgtaggctc cctgcagctg
      421 cgccggggtg tagccacagg gcgcgtaggg ttgagcttcc ccataggctg tgggcagatc
      481 cgtcgcgatc ttctcggccc agtaggtcga gcagggcggc gcgttgcgga agccggcgga
      541 gggcggcgcg tcggcgctga ggttgtaggg gtgcgtcagc gcctgcgtct ggtcgatgcc
      601 gatgatccca gcgacgctgc cggccagcgc cgcggggatg ctcaacgggc cgtccggcgc
      661 gcgcagcgtg gcgccggcga cggtgtactc gttgagctgg acgccgaagg cggactccac
      721 ctgggcgacc gtgccctcgg ccgagatgta gtggttgttc tggggggtgt agaccaccga
      781 gaagccctgg ctctgcagcc aggagcgcac cgagttggta tccccggcgc tgggcgcgaa
      841 ctgctggcgg aactgcgccg gcgtcaggta gttgccgtag ctcgggctgc tcgggtcgga
      901 gacggcccgc gccagcgcgg ccgcctggtc accgccgcgc cagccgaggt agacgcggaa
      961 gccgacggag tcgctcgtgt tggatgacga cttgaagttg gcggaagtgg cccacggcgg
     1021 cacgctcccg gccagtgtgg cccgcccgga gggcgccgcg gcggcaggcg tgatccccag
     1081 cgacagcagc agcaccagtg cccaaagcac cagtgccggc cgtttgatcc cccgtctcat
     1141 gccaggttct cccttcacac gtgtcgcccc tggcgacgtc ccagtcatac cgccccagcg
     1201 cgatcgtcga accgggagcg ccgatcgccc cgctgcgccg cgtggcccgg ccgaaacggt
     1261 cccagacctt ccgacgccga cgacctgcgc ccatcgtgct gctgacctcc cctccagtcc
     1321 cggccggcac accggcgatc gcgtccagtg ggcggaaatg accgggacac cgtacaagca
     1381 accgggcgag acggcaagat tcggcttgat tctccgatca ggtatgcaag gtgagagtgg
     1441 tgtgggtaga ctacgcggcc gtttgttgac ttgcgtggca tggcaagcta accgcgcgcg
     1501 aagatgaaga tacgccggcc aaccattacg tttgcgtcgt gcagagccgt ggccaattcg
     1561 tactcccgcg cgctgccccg gcgcgggggt gacgcgcatc cgcaatcccc actcacggcg
     1621 cgcgcgtggc cttcgagtcg gccagcttgg gggtagaatt ggtcgcaagc gagaactcgt
     1681 agctgaagcg cgcgccggat ccctgtgccg cggagtgggt gtccgtcaag ttcggcgcgg
     1741 cgcggtcact gccatgtcga ccagaccgcc ggcgtttggc gagctgttgc agcgttaccg
     1801 ggtgcgggcc gcgctcagcc aggaggagct ggccgagcgc gcggggctct ccgcctcggc
     1861 catcagcgcc ctggagcgcg gcgcccgcca gcgcccctac ccgcacacgc gccagctgct
     1921 ggcgcaggcg ctggggctgt caccggacga ggcggcggtg ttcacgggac cagcgcgcgg
     1981 gcgagcggcc gtgccttctc cgtcctcccc cacgaccaac ctgccggcgc ggcgcacgcc
     2041 gctgatcggc cgggaggaag cgagcgtgga gctgcgcgcg ctggtgcggc aggacgcggg
     2101 gcggctgctg acgctgaccg ggagcgggac gccctcgtcc tggcacgctc ctgtgatgat
     2161 cagccggtcg cggtcgaagc cctggcctat ctcgccacgg tgcggcgaca cgcgggcctg
     2221 ctgaaggagt ccgcacgctt gctgggggag tgcctggcgg ctgcgcaggc gatcggcgac
     2281 gaggcgaggc tcgcgttcgc gctcctgtcc ctgggtgtga cggtgcgtgt gcagcgggat
     2341 atcgcgcgct cggtggcgct cctggaggag agtctggagc gctttcagcg cctgggcgat
     2401 cttcgttgga tcgccaacgc gcagacgatg ctgggctcag cgctggtgca ccagggggag
     2461 tcggatcgtg cggcggccac cctgctggcc gggctcgccg ggcatcgcgc cgtcggtgat
     2521 ctgaccttca gcacgtcggg gttgctctgc ctggccgccg tgctggcgga gcgagcgcag
     2581 cccgcgtggg cggcgagcgt gtttggcgcg ctgctggccc tcaacgaacg agtcgggacg
     2641 acgctgacga cgggaaatca gcgcgaatac gaccgcatcg cgtccggggt gcgggcacag
     2701 ctcggtgcgg cggactttgc ttggactctg gccgaggg
//
LOCUS       DASUBI010000111         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1304405, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000111 DASUBI010000000
VERSION     DASUBI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304405"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..1596
                     /locus_tag="VFU72_02140"
     CDS             448..1596
                     /locus_tag="VFU72_02140"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5422314.1"
                     /translation="MTTSKRASTSEGQDSDAQRRFPAIFDGHNDTVISLAGEGFDGPG
                     GRSFFERSDKGHIDLPRARAGGLGGGFFAVLARGKQPSADPALSTTGSKEITGIGTGF
                     SDEEGWPPPIPLEEAQSDAVMRLGDLIRLARQSNGQAQIVTSAAELQHCLDNGIFAIE
                     LHFEGADPLDLDGRALETFYNAGLRSVGITHFRQNQYAAGVPNRFPGSPDIGPGLTDA
                     GKELVRQLNRMHVLVDLSHANEKTFWDVATISDAPLVATHSNAWALSNAPRNLTDKQL
                     AAIRESNGMVGLNYHVGFLRADGEMNPKTPLSVMADQIDYLVEKLGIDCVGLGSDFDG
                     AMMPAELKDAAGLPRLMQALADRGYGDAELTKIAHGNWVRVLRATWGE"
     gene            complement(2040..>2460)
                     /locus_tag="VFU72_02145"
     CDS             complement(2040..>2460)
                     /locus_tag="VFU72_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422315.1"
                     /translation="LSNMYIAVAVVAWIVYARLIRSEILVQKEQEYVIAAKAMGMGDV
                     RLILRHLMPNTITTCIVYAMADIALYILLAAALSYLGLGAKPPTPEWGAMITEGQSYM
                     TTAWWMSALPGLAIIVTGIGLSLIGDGLSDILRPGTR"
BASE COUNT          405 a          856 c          852 g          347 t
ORIGIN      
        1 ctcgcgcgac gggcgcggcg acgtggacgg cttcgacatc tacaccgacc gcatccgcta
       61 cctgcgcttc gcgcgcgagg cgtgagccac acatgggcag catcggcggg caaaagggcg
      121 ccattctccg ctcaggtagg ggcggccccc ccgtggccgc cctccccagc cacggcgcgg
      181 ccagggggac acggcgcacg tgcggcccag cggcgcggcg acgggcacgg ccgtagggcg
      241 aacagggcgg ccacgggggg gcgcccatac cgggtgaagg cggggcgctt cctctggtag
      301 gggtgatccg catcgtcatc gtccggagcg ccgtcggcag gtagccgtgg ctgccggcca
      361 cgtcgtagtc ggtgttgaca gcccccatgt gcgcgggtac aacagcacgc gcagactcgc
      421 gaatcaaccg gagagcagga gcaccacatg accaccagca agcgcgcgag cacgagcgag
      481 ggccaggata gcgacgccca gcggcgcttc ccggccatct tcgatggcca caacgacacg
      541 gtcatcagcc tggccggcga ggggttcgac ggcccgggcg gccgctcctt cttcgagcgc
      601 agcgacaagg gccacatcga cctgccgcgc gcccgcgcag gcggcctggg cggcggcttc
      661 ttcgccgtac tggcgcgtgg taagcagccg agcgccgatc ccgccctgag cactacgggg
      721 tcgaaggaga tcaccggcat cggcaccggc ttcagcgacg aggagggctg gccgccgccg
      781 attccactgg aggaggcgca gtcggacgcc gtgatgcgcc tgggcgacct gatccgcctg
      841 gccaggcagt cgaacgggca ggcccagatc gtgacctcag ccgccgagtt gcagcactgc
      901 ctggacaacg gcatcttcgc catcgagctg cacttcgagg gcgccgaccc gctcgacctc
      961 gacggccgcg cgctggagac cttctacaac gccgggctgc gctcggtcgg gatcacgcac
     1021 ttccgccaga accaatatgc cgcgggcgtg cccaaccgct tccccggctc gcccgacatc
     1081 ggtcccggcc tcaccgacgc cggcaaggag ctggtgcgcc agctcaaccg catgcacgtg
     1141 ctggtcgatc tctcgcacgc caacgagaag accttctggg acgtggctac catcagcgac
     1201 gcgccgctgg tggccaccca ctccaacgcc tgggcgctct cgaacgcgcc gcgcaacctg
     1261 accgacaagc aactagccgc catccgcgag tccaacggca tggtcggcct caactaccac
     1321 gtcggcttcc tgcgtgccga cggcgagatg aaccccaaga ccccgctctc ggtcatggcc
     1381 gaccagatcg actacctggt cgagaagctg gggatcgact gcgtcgggct cggctccgac
     1441 ttcgacggcg cgatgatgcc agccgagctg aaggacgccg ccgggttgcc caggctgatg
     1501 caggcgttgg ccgaccgcgg ctacggcgac gcggagttga ccaagatcgc gcacggcaac
     1561 tgggtgcgcg tcctgcgcgc cacctggggc gaatagggcg cgtactccca tcccccagcg
     1621 tgcccggcgg cgaggttggg tgccgggtgc ggacggatcc ctcgactgcg ctcgggatga
     1681 ccgggagagt gcggtgcgga cgaggaggat gagcgtcgaa cgacgatcag cctcgtccgc
     1741 taggcctcat cccctcccct tgtcatcccg agcttgtcga aggatcgtgc tcgatggcgt
     1801 cctgcgacgt cgtcggccac cgcccggcct ccgcactggc actccgcgca gggaagtggc
     1861 cggtcgacag ggtgagtgtc gcccggagca cggatccttc gcctgcgctc aggctgacaa
     1921 gtgaaaggga ggggcacacg acagcggcag ccttcgtcgt acgcccgtat ttccccggtc
     1981 atccccggcg gagtcgaggg gcccgacgtg atgcgccgct cagcgaggcc ggcgcgtcgt
     2041 cagcgggtgc cagggcgcag gatgtcggag agaccgtcgc cgatcaggct gaggccgatg
     2101 ccggtgacga tgatcgccag acccggcagc gcgctcatcc accaggcggt ggtcatgtag
     2161 ctctgcccct cggtgatcat cgcgccccac tcgggggtcg gtggtttggc gccgaggccc
     2221 aggtagctga gcgcggcggc cagcaggata tagagcgcga tgtcggccat cgcgtagacg
     2281 atgcaggtcg taatggtgtt cggcatgagg tgacgcaaga tcaggcgcac gtcgcccatg
     2341 cccatggcct tggcggcgat gacgtactcc tgctccttct gcaccaggat ctcgctgcgg
     2401 atcaggcgcg cgtagacgat ccaggccacc accgcgaccg cgatgtacat gttgctcagg
//
LOCUS       DASUBI010000112         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_445725, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000112 DASUBI010000000
VERSION     DASUBI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445725"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFU72_02150"
     CDS             complement(<1..507)
                     /locus_tag="VFU72_02150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5422316.1"
                     /translation="MIYASARLASIGARLFVSLFIAFGALLAASFMPTAIPSAGATVQ
                     LFECAQGAMPFPVSVDSGAKLGWIYDEPNPPTTDKRDDDSPTAHTGIDIRLKLGTPIY
                     ATGTGTIERRTDSATHVWYPDVVVSFPNGTTVKGIDVYIAHMTSVTNPGTVERGDVIG
                     YSSGSNGLN"
     gene            complement(792..1973)
                     /locus_tag="VFU72_02155"
     CDS             complement(792..1973)
                     /locus_tag="VFU72_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422317.1"
                     /translation="MSGELAALRERHARYFLALAESAPPYVPEVRRGAWYTRVDANHD
                     NFRAALTWASTLADPELLIRLSAALWPYWHEYMHVEEGRRWLSAALDRADDAPAALRA
                     ALLTGDCTLAFRQADYQVAFAHAHAALALWQQLNDQHGQALVLQQLGWGSFLTGNFGN
                     AIDLFAAEVEQWRLVGDADGLARGLSDSASAHYMSGDLAGAMPFISEGMALLRHARDE
                     MGLARSACDRGLHALLREDLADAMPLLREAVDRFRSADRHYQLGTTIFYLGTALCFAG
                     QLAEAGDCYSEALRLHDEVDDRVGLSLTLLGFAAVAHRRGDGTRAATLCGASHAVQHA
                     HHITLPPAVQALYQREVSLVIEQIGPERFAEAFEQGSMLTVEEAVAMARAAAGRLEFL
                     D"
     gene            complement(1970..3328)
                     /locus_tag="VFU72_02160"
     CDS             complement(1970..3328)
                     /locus_tag="VFU72_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEU5422318.1"
                     /translation="MGLVVHSAAGTATQSPLAVLLTQHMTERGLTQARLAHLTGLSKA
                     TLNRWLSGKSDHPYHHLGVLRLAAALGLRKVETSRLLRAAGLPPLDALAASAEPEERA
                     LLARWAAPERHHLPADLTSFVGREDEIVTVAELLCRDGLRLLTLTGPGGSGKTRLALR
                     VARETLDVFPGGVFFVPLGAVAEPGLLLKSIAERVGLRDVLDSALPARLTGWLRGKRV
                     LLLLDNLEQLLESGPAIAGLLRASPGLTVLATSRVPLHLSGEHEWPLLPFPLPAPEEP
                     GARLRANPAVRLFIERAEAANPRRRLDDEDLPAVAEICARLDGLPLAIELAAAGTRAR
                     VPWRLLADFPSRLDLAGDGPRDLPQRQRTLRNTIAWSVELLPEPARRLFPRLAVFAGS
                     WDAEAAAAVCAPEGTPVEAVEGFLRTLVDASLIQSAPAAVGTPRYRMLETIREFGLER
                     LR"
     gene            3512..4879
                     /locus_tag="VFU72_02165"
     CDS             3512..4879
                     /locus_tag="VFU72_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422319.1"
                     /translation="MGSELARVIERGAAERLPLRTKLLWGWAGLGAEALTQSRHAWLV
                     YFYAPPAGAGHAARLPLLTVTLLLFAGKLFEAGADTLVGYWSDRTRSRLGRRIPFVLL
                     GGPPMALFAVLLFAPPPAHGAALAAWFFVALELFFVFQSIVGVPYEALLPEIARSSDE
                     RVALSAWRVAFGVLGAGVGLVGSGLLISQFGFRAMALVIALLAVAGRYVGVAAVWRRV
                     ERGRVPAPPSLRVTLRLTLANRGFLVFMLSFVLFSSALAMLTGLLPYYVSSLLGERDT
                     GTWAGLLTAVAIGAMALAIPLFARLARRTSQRHAYRRAMLLSACAFPVLFVAGSLPGP
                     APLLQALVAMVIVGAPLAGVFLFPGPIIADLCDADAAATGLRREGMFYSVQSFMDKLT
                     GACAPLLLGLILLLGDSPGQLLGIRLVGPAAGLLVLAGYLALRAFERAEQAAPALEPQ
                     ALAGR"
BASE COUNT          736 a         1760 c         1846 g          800 t
ORIGIN      
        1 attgaggccg ttcgaaccgc tcgaatagcc tatgacatct cctcgctcca cggtgccggg
       61 gttggtcacg ctcgtcatat gagcgatata cacatcaatg cctttgacgg ttgtgccatt
      121 cgggaagctg actactacat ccggatacca cacgtgcgtt gctgaatccg ttcgccgctc
      181 aatcgttccg gttcccgtag catagattgg tgttcccaat ttcaatcgaa tgtctatgcc
      241 ggtgtgcgcc gttggagagt cgtcatcccg tttgtcggtg gtgggcgggt ttggctcatc
      301 ataaatccag ccgagtttgg cacccgaatc aactgagaca gggaagggca tagcgccctg
      361 tgcgcattca aatagctgta ccgttgcccc ggctgacggg attgctgttg gcataaacga
      421 agctgccaac agcgctccga acgcaataaa tagggaaacg aatagccgag caccaatcga
      481 tgctaaacgc gcactcgcgt agatcacatc gcccccctat aaccgacatg catcgagcgt
      541 ccgccggtga gacgagcgcg gttccacatc cgttggcccc gcggtcgcca aaatctgacg
      601 acgacagtcg cttgggaagg gtgctcgccc tcatctctag atgacaatag aggaggactg
      661 aagccatgag cgctacgaag cgcaaactga aaccaaatga gacgctcagg gtactctgac
      721 tcggcgacca accgcgacga tgaaggcgca atgaacgcgc ggagcagcac aggagcgagg
      781 ttgaggtgtg ctcaatccaa gaattctaag cgacccgctg ccgcacgtgc catcgccacc
      841 gcctcctcca ccgtcaacat gctgccctgc tcgaacgcct cggcgaagcg ctcggggccg
      901 atctgctcaa taacgaggct cacctcgcgt tggtagagcg cctggacggc gggggggagg
      961 gtgatgtggt gcgcgtgctg gacggcgtgg gaggcgccgc agagggtggc ggcgcgcgtg
     1021 ccgtcgccgc gacggtgggc cacggcggcg aagccgagga gcgtgagcga caggcccacc
     1081 cggtcatcca cttcgtcgtg cagccgcagg gcttcgctgt aacagtcgcc ggcttcggcg
     1141 agctgccccg cgaagcagag cgcggtgccc agatagaaga tcgtcgtccc caactggtag
     1201 tgccggtcgg cggagcgaaa acggtcgacc gcctcgcgca gcagcggcat cgcatccgcg
     1261 aggtcttcgc gcagcagcgc gtgcagtccc cggtcgcagg ccgagcgggc caggcccatc
     1321 tcgtcccgcg cgtggcgcaa gagggccatg ccctcgctga tgaacggcat ggcgccggcc
     1381 aagtcaccgg acatgtagtg cgcggaggcc gagtcgctga gtcccctggc aagcccgtcc
     1441 gcgtccccga ccagccgcca ctgctcgacc tccgccgcga acaggtcgat ggcgttcccg
     1501 aagttgcccg tcaggaagct gccccagccg agctgctgga gcacgagcgc ttgcccgtgc
     1561 tgatcgttca gctgctgcca gagcgccagc gccgcgtgcg cgtgtgcgaa cgcgacctga
     1621 tagtcggctt gcctgaacgc cagggtgcag tcgccggtca gcagcgcggc gcgcagcgcg
     1681 gcgggcgcgt cgtcggcgcg gtcaagagcc gcagacagcc agcggcggcc ttcttcgacg
     1741 tgcatgtact cgtgccagta gggccagagg gcggcgctca ggcggatgag cagctcggga
     1801 tcagccaggg tgctggccca ggtgagggcg gcgcggaagt tgtcgtggtt ggcgtcgacg
     1861 cgcgtgtacc aggcgccgcg ccgcacctcg ggcacgtagg gcggggcgga ctcggccagc
     1921 gccaggaaat agcgcgcgtg gcgctcccgc agcgccgcca gctcgccgct caccggaggc
     1981 gctccaaacc gaactcgcgg atcgtctcca gcatgcggta gcgaggcgta ccgacggctg
     2041 cgggggccga ctggatcagg ctggcgtcca cgagcgtgcg caggaagccc tccacggcct
     2101 cgaccggcgt gccctcgggg gcgcagacgg cagccgcggc ctcggcgtcc cagctgccgg
     2161 cgaagacggc caggcgcggg aacaggcggc gcgcgggctc cggcaagagc tccacgctcc
     2221 aggcgatggt gttgcgcagg gtgcgctggc gctggggcag gtcgcgcggg ccgtcgccgg
     2281 ccaggtcgag ccggctgggg aagtcggcca agaggcgcca gggcacgcgc gcgcgcgtgc
     2341 cggcggcagc cagctcgatc gccagcggca ggccgtcgag ccgggcgcag atctcggcca
     2401 ccgccggcag atcctcgtcg tcgagccggc ggcgcgggtt ggccgcctcg gcgcgctcga
     2461 tgaagagccg caccgccggg ttggcgcgca gccgggcgcc gggctcttcc ggcgccggca
     2521 gcgggaaggg gagcagcggc cactcgtgct cgccggagag gtgcagcggc acccggctgg
     2581 tggccagcac ggtgagaccc ggtgaagcgc gcagcagccc ggcgatcgcg gggccgctct
     2641 caagcagttg ctccagattg tcgagcagca gcagcacgcg cttcccgcgc agccagccgg
     2701 tcagccgcgc cggcagcgcg ctgtcgagca cgtcgcgcag gccgacgcgc tcggcgatgc
     2761 tcttgagcag caggccgggc tccgccaccg cgcccagcgg tacgaagaag acgccgccgg
     2821 ggaagacgtc cagcgtctcg cgggctaccc gcagcgccag gcgtgtcttg ccgctgccgc
     2881 ccgggccggt cagcgtcagc agccgcagcc cgtcgcggca gagcagctcg gcgacggtca
     2941 cgatctcgtc ttcccgcccc acgaacgagg tgaggtcggc ggggaggtgg tggcgctccg
     3001 gcgccgccca gcgcgccagc agcgcccgct cctcgggttc ggccgaggcg gccagcgcgt
     3061 cgagcggcgg caggccggcg gcgcgcagca ggcggctggt ttcgaccttc ctcagcccga
     3121 gcgcggcggc caagcggagc acgccgaggt ggtggtaggg gtgatccgac ttgccgctga
     3181 gccagcggtt gagcgtggcc ttggagagtc ccgtcagatg cgcgagccgc gcctgggtca
     3241 ggccgcgctc ggtcatatgc tgagtcagca gcacggccag cggcgattgc gtggccgtcc
     3301 cagcggcact atgtaccacc agtcccatac ccttccccat attcgcttgt cccccgattc
     3361 ctcgtggcgc tgattctaca cgaggctggg agtacaatcg agaccgttgc ggacaacggt
     3421 tgaggatttc gcaggtgacg aggtccgtgg ccggcgctgc gccagcgagg ggcgccgcgt
     3481 gtggcgggca cgcgtggcga agggttcagg gatggggtcg gagttggcgc gcgtgatcga
     3541 gcggggcgcg gcagagaggc tgccgctgcg caccaagctc ctgtggggct gggccggact
     3601 cggcgcggag gcgctgaccc agagccggca cgcctggctg gtctacttct acgcgccccc
     3661 ggcgggcgcg ggtcacgccg cgcgactgcc gctgctgacc gtcaccctcc ttctcttcgc
     3721 cggcaagctc ttcgaagccg gcgccgatac gctggtcggc tactggagcg atcgcaccag
     3781 gtcgcgcctg ggtcggcgca tccccttcgt gctcctcggc ggcccgccga tggcgctctt
     3841 cgccgtgctc ctcttcgctc cgcccccggc gcacggcgcg gccctggccg cctggttctt
     3901 cgtcgcgctg gagctgttct ttgtctttca gagcatcgtc ggcgtgccct acgaggcgct
     3961 gctgccggag atcgcgcgct caagcgacga gcgcgtggcg ctctcggcct ggcgcgtcgc
     4021 cttcggcgtg ctcggcgccg gggtggggct ggtcggcagc gggctcttga tcagccagtt
     4081 cggcttccgc gccatggcgc tggtgatcgc cctgctggcc gtggcggggc gctacgtcgg
     4141 ggtggcggcc gtctggcggc gcgtcgagcg cggccgtgtg cccgcgccgc cgtcgctgcg
     4201 cgtcacgctg cggctgacgc tggccaaccg cggcttcctg gtcttcatgc tctcgttcgt
     4261 gctcttcagc agcgcgctgg ccatgctgac cggcctgctg ccctactatg tgtcgagtct
     4321 cctgggcgag cgcgacaccg gcacctgggc cgggctgctg accgcggtcg ccatcggcgc
     4381 catggcgctg gcgatcccgc tcttcgcgcg gctggcccgg cgcacctcgc agcggcacgc
     4441 ctaccgccgc gccatgctcc tctcggcttg cgccttcccg gtgttgttcg tcgccggctc
     4501 gctgcccggc cccgcgccgc tgttgcaggc gctggtcgcc atggtcatcg tgggggcgcc
     4561 gctggccggc gtcttcctct ttcccggccc gatcatcgcc gacctctgcg atgcggacgc
     4621 tgccgccacc ggcctgcggc gcgaggggat gttctacagc gtgcagtcct tcatggacaa
     4681 gctgaccggc gcctgcgcgc cgctgctgct ggggctgatc ctcctcctgg gcgatagtcc
     4741 cggccagctg ctgggcatcc ggctggtcgg tccggccgcc ggactgctgg tgctggccgg
     4801 ctacctggcc ctgcgcgcgt tcgagcgcgc tgagcaagcc gcccccgcgt tggaacccca
     4861 ggctctggct ggccgatagc acggctcttg caggtgcccg cgtacgccag ccgtccggac
     4921 ggcggctggt ggcgtggtag actggtgtgt tggctatcgc aggtgcgcgc gcagaagggc
     4981 gaagcgtgac cattcagttg aagcggccgg agcagatcga gcgcatccgg gacgcgggca
     5041 agatcgtggc cgagacgctg gcgctccttg ccggcgaggt gcgcccgggc gtgaccaccg
     5101 ccgagctgga cgccatcgcc gagcgcaaca tccggcgcca cg
//
LOCUS       DASUBI010000113         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_746667, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000113 DASUBI010000000
VERSION     DASUBI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746667"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(533..1453)
                     /locus_tag="VFU72_02170"
     CDS             complement(533..1453)
                     /locus_tag="VFU72_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEU5422320.1"
                     /translation="MTARLTRKGGGAARLRELLVSGQMIVAPGAFNALAARCVEEVGF
                     EAVYMTGFGTAAALLGRPDVGLATMTEMVDNAARMAACVDIPVIADADTGYGNPINVI
                     RTVGAYETAGVAGIHIEDQVAPKKCGHMDGKLVIAADDMVQKIRAAADARSDPDFVII
                     ARTDAVAVEGLDSAIDRAGHYQAAGADVLFVEAVRTEEEAAAVARAFPGVPLLFNWAE
                     GGKTPPIGLGRLRELGYRIVIFPISTLLAATGAMRSILAEIAAAGTPAAALPGLPSFG
                     EFVDFIGLPEVRAAEQRYAATRTPAVTERA"
     gene            complement(1459..1845)
                     /locus_tag="VFU72_02175"
     CDS             complement(1459..1845)
                     /locus_tag="VFU72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5422321.1"
                     /translation="MSYVYDAGALIAIDGNDLEMWAIHKAALAENREIVIPAIVVGQA
                     WRDGSKQAQLARFLRTCQIEPTDLQTAKAAGVLCGKAGTSDVVDATVMVTALAHYAII
                     VTSDHKDMSKLAEACGARPIPVVIRV"
     gene            complement(1848..2114)
                     /locus_tag="VFU72_02180"
     CDS             complement(1848..2114)
                     /locus_tag="VFU72_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422322.1"
                     /translation="MATKKVTVTLPTDLVELVNNAAAEAGIPVSRFVSSALERELRRR
                     VGLALVAEWEAEHGPITPAQMAAARAKMAAADAEYLAQTEQRTA"
     gene            2210..>3167
                     /locus_tag="VFU72_02185"
     CDS             2210..>3167
                     /locus_tag="VFU72_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5422323.1"
                     /translation="MAELVGDLDLDEFDFPSAELTGDFYHQQLAALSEHGWLVKSPIS
                     YVVLDRESGEFFLRARQTRFPGKEIAELFGITGGRLAEQIEANILNLTGDQHRRLRGL
                     VSPAFTPRAAQRWRPVMRAIAADLWADLNGAAECEFVSAFAGPFPARTIAAVIGAPQT
                     DAAKLQEWSGWVQRQFDIKALAEQPQRIEQAVNELYDYVASLLERRRREPADDLLDAL
                     LKAEEQGDRLSPAECVNLVLNVLAGGVDTTVAQMAHGMRLFALHPEQWELLRQGPELV
                     SKAVDEVLRFEPITPFTARICTDQIEHRGVIFPEGTIVAICSE"
BASE COUNT          479 a         1074 c         1079 g          535 t
ORIGIN      
        1 gctgcgaacg agaccgaact gcgggtccag gccagcttgg tgcgttcgcg cgctagcccg
       61 gggtcggcgt cctcgatgtc atcggggacc tcagcccggc gcggtgccgg cccttgccag
      121 ggcggcggtc tcaccggcgc gtcaccttcg agtacaggtc gaccgcagcg gcgagcaggg
      181 cgatgatccc gacagtcgcg gcaagcatcc tgggaagctg gctcggcggc aatggcgcgc
      241 cctgcctgat tgcccgctgg ttgcgccgcc attcgacgta gctgagcggc gacagcgccg
      301 ctccaagcaa gatcagcggg gtcgcgatcg ctgatcggcc ccatggcacg tgcggaaatg
      361 gcggcaggag ttgagcgacc gcgaggccgg cagcgatcag ggcgagcgcg gtgcggctcc
      421 aggcgagaaa cgttcgctcg ttcgccagcg taaatctgac gtcgggctcg gtgccctcag
      481 cgccggcgtc gcgtgcgtct ggctgcgggt caggtgtgga cgtgactaac tcctacgcac
      541 gttccgtgac agccggcgtt ctggttgcgg cataacgctg ctcggcggcc ctgacttcgg
      601 gcaggccgat gaagtcgacg aactcgccga acgacggcag gcctggcagg gccgcggccg
      661 gcgtgcccgc cgcggcgatc tcggcgagaa tcgagcgcat cgccccggta gcggcgagca
      721 acgtgctgat cggaaagatg acgatccggt agccgagttc gcgcagccgg ccgagcccga
      781 tcggcggtgt cttgccgccc tcggcccagt tgaacagcag cggcacgcct gggaacgccc
      841 gcgcgacggc tgccgcctcc tcttccgtcc tgaccgcttc gacgaacagc acgtcggcgc
      901 cggcggcctg gtagtggccg gcccggtcga tggcgctgtc cagcccctcc acggccaccg
      961 cgtcggtgcg ggcgatgatc acgaagtcgg gatcgctcct ggcgtccgcg gccgccctga
     1021 tcttctgcac catgtcgtcg gccgcgataa caagcttgcc gtccatgtgc ccgcacttct
     1081 tgggcgcgac ctggtcttcg atgtggatcc cggcgacgcc ggcggtctcg tacgcgccga
     1141 ccgtgcggat gacgttgatc gggttcccgt agccggtgtc ggcgtcggcg atcaccggga
     1201 tgtcgacgca ggccgccatc ctcgcggcgt tgtccaccat ctcggtcatc gtcgccaggc
     1261 cgacgtcggg ccggccgagc agggccgccg ccgtgccgaa cccggtcata tagaccgcct
     1321 cgaagccgac ctcctccaca caccgcgcag ccagcgcgtt gaacgcgccg ggagccacga
     1381 tcatctggcc cgagacgaga agttcgcgca gccgcgccgc gccgccacct ttccttgtca
     1441 gccttgccgt catggaagtc agactcggat gacgaccggt attggccgcg caccgcaagc
     1501 ctccgccagc ttgctcatat ccttgtgatc gctggtgacg atgatcgcgt agtgcgccag
     1561 cgcggtcacc atcacggtgg cgtcgacgac atccgaagtg cctgctttac cgcacagcac
     1621 gccggccgcc ttcgcggtct gcaagtcagt cggctcgatc tggcaggtac gcagaaagcg
     1681 ggcgagttgc gcttgcttgc taccgtctcg ccacgcctgg cccacgacga tcgccgggat
     1741 gacgatctct cggttctctg ccagcgccgc cttgtgaatc gcccacatct cgaggtcgtt
     1801 gccgtcgatc gcgatcagcg ccccggcgtc gtagacgtac gacacgttca cgccgtgcgc
     1861 tgttcggtct gcgcgagata ctccgcgtcc gctgccgcca tcttggcgcg ggctgcggcc
     1921 atctgggctg gagtgatcgg gccgtgttcg gcctcccatt cggccacaag ggctaggccg
     1981 acacgtcgcc gcagttcgcg ctcgagcgcg ctgctgacga agcgcgacac cggaatgccg
     2041 gcctcggcgg ctgcgttgtt caccagctca acgaggtcag tcggcagtgt taccgttacc
     2101 ttcttagtgg ccatgctttc accatatcat gatatggcct agtgcgcggg ctggtggtag
     2161 cgatagcttt tgcttgcgag cgggcgcatt gagggaggta cggcacgcga tggctgagct
     2221 ggtcggggac cttgatctcg acgaattcga cttccctagc gccgagctga ccggagactt
     2281 ctatcaccag cagctcgcgg cgctcagcga gcacggctgg ctggttaagt cgccgatctc
     2341 ctacgtggtg ctcgaccgcg agtccggtga gttcttcctg cgggccaggc agaccaggtt
     2401 cccaggcaag gagatcgccg agctgttcgg catcaccggc ggccggctgg ccgagcagat
     2461 cgaggccaac atcctgaacc tcaccggtga ccagcatcgg aggctgcgcg gcctggtgtc
     2521 gcccgcgttc acgccgcgcg ccgcgcagcg gtggcggccg gtcatgcggg cgatcgcggc
     2581 cgacttgtgg gccgacctca atggcgccgc cgagtgcgag ttcgtctcgg ccttcgccgg
     2641 gccgttcccg gcgcggacca tcgcagccgt gatcggtgcg cctcagaccg acgcggcgaa
     2701 gctgcaggaa tggtcgggct gggtgcagcg gcagtttgac atcaaggcgc tcgccgagca
     2761 gccccagcgg atcgagcagg cggtcaacga actttacgac tacgtggcca gcttgctcga
     2821 gcgccgccgc cgcgagccgg ccgacgacct gctcgacgcg ctgctcaagg ccgaagagca
     2881 gggcgaccgg ctgtcgcctg ccgagtgcgt caacctggtg ctcaacgtgc tggccggcgg
     2941 tgtcgacacg acggtggcgc agatggcgca cggaatgcgg ctcttcgcac tccaccctga
     3001 gcagtgggag ctgctgaggc aagggcccga gctggtcagc aaggccgtcg acgaggtgct
     3061 caggttcgag ccgatcacgc cgtttaccgc caggatctgc accgaccaga tcgagcaccg
     3121 cggcgtgatc ttccccgagg gaacgatcgt cgctatctgc tctgagc
//
LOCUS       DASUBI010000114         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_801389, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000114 DASUBI010000000
VERSION     DASUBI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801389"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /gene="acs"
                     /locus_tag="VFU72_02190"
     CDS             <1..1884
                     /gene="acs"
                     /locus_tag="VFU72_02190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5422324.1"
                     /translation="SPEIYASAQEDPDGFWAGVARELDWYQPWERVLNWDNPPKVEWF
                     VGGKINAAYNCVDRHAKGAKADKAAIIWEGEPGDHRTLTYADLYREVNRAALMLKDLG
                     VGKGDRVAIYLPMIPELPIIMLACARLGAPHSVVFAGFSPDSLAGRINDCEAKLLVTA
                     DGGWRRGNVVPLKENADKAVADTPSIEHVLVVQRVGPERAPIQMKQGRDLWWHEQIGK
                     VQGNYVEPEQLDAEHPLYILYTSGTTGKPKGVLHTTGGYLVGTYSSCKWVFDMKDSDI
                     FWCTADIGWVTGHSYVVYGPLAYGVSQVMFEGTPDYPDKDRFWDIIERHRVTVLYTAP
                     TAIRGFMRWGRQYPEMHDLSSLRLLGTVGEPINPEAWVWYNDVIGHGRCPVVDTWWQT
                     ETGAIMISDLPGVTVAKPGSAGPPMPGIVPDVLDNDGNPVPAGHGGFLVIKRPWPSMF
                     RTIWGDWDRYVETYFGKYGPKIYVTGDGAKRDEEGYYWLMGRVDDVLNVAGHRLGTME
                     LESALVSHPSVAEAAVIGVSDALKGQAVAAFVTPRLGVEPSSKLSEELRRHVAEKIGP
                     IARPDRIYFTAELPKTRSAKIMRRLLRDVAEGRVMGDTTTLTDATVIEEIKRNYEQNE
                     GGE"
     gene            complement(2012..2374)
                     /locus_tag="VFU72_02195"
     CDS             complement(2012..2374)
                     /locus_tag="VFU72_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5422325.1"
                     /translation="MISRGDWASEEGKTFTGPTRLERLLPAQQEQGAALSLVRFRDGA
                     VTNWHTHPGEQVLVIVEGQGRAGSESETMTFSTGDVVYTPPGQRHWHGAQAGHDMAHL
                     SFTTGGPPSWEGPPDVSR"
     gene            2495..2902
                     /locus_tag="VFU72_02200"
     CDS             2495..2902
                     /locus_tag="VFU72_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEU5422326.1"
                     /translation="MNMERQVTGAPGSLLETLAMTIVERVPERVVVTMPVTSAHHQPL
                     GYLHGGASVALAETAASVAANLNCGPDKVAFGQEINANHLRPIRDGLLTATATPLHIG
                     RTSQVWDVKIRDEQERLVCVSRCTLAAVPRNRQ"
     gene            complement(2909..3391)
                     /locus_tag="VFU72_02205"
     CDS             complement(2909..3391)
                     /locus_tag="VFU72_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422327.1"
                     /translation="MREIVIGEWSLRVDPEATKDRYRSSASLPCSCRCRYCRNFVAAR
                     ESVYPEELLAVLEQLGVDRTREAETQEVAEVGPGWHHYIGHFPFVGEVLEGVDPWRPI
                     PMGDRAAIWFNGATVPMTPSMRDAFADQPVMLLRFAVTVPWVLSAPPSRKPAGHATRN
                     "
     gene            complement(3614..>4184)
                     /locus_tag="VFU72_02210"
     CDS             complement(3614..>4184)
                     /locus_tag="VFU72_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEU5422328.1"
                     /translation="AAAEIVGTEITDQRLNRGIAGAAAGSMVSALFGGFATTAYAQNV
                     GLLRLTKIGTRHAVTYAGAIFLVLAFVPKLGALLAATPDPVVGGIFLPAAGSLIVVGI
                     DLLAKMAPTERNYMVAGLAVMLGVGLPLMGDPLFSKLSRDWSLLLSQQIVVGAVVAIV
                     LQLALVQIPDWIGARGSAKQEDPKPEPAD"
BASE COUNT          694 a         1381 c         1482 g          627 t
ORIGIN      
        1 agccccgaga tctatgccag tgcgcaggag gatcccgacg gcttctgggc cggcgtggcc
       61 cgggaactgg actggtatca gccgtgggag cgcgtgctca actgggacaa cccgcccaag
      121 gttgagtggt tcgtcggcgg caagatcaat gccgcgtaca actgtgtcga ccgtcacgcc
      181 aagggcgcca aggccgataa ggccgcaatc atctgggagg gcgagccggg cgaccaccgc
      241 acgctgacct acgccgacct ctaccgcgag gtcaaccgcg ccgcgctgat gctgaaggac
      301 ctgggtgtcg gcaagggtga ccgcgtggcg atctacctgc cgatgatccc cgagctaccg
      361 atcatcatgc tggcctgcgc ccgcctgggc gcgccgcact ccgtcgtctt cgccggcttt
      421 agccccgatt cgctggccgg acgcatcaac gactgcgagg ccaagctgct ggtgactgcc
      481 gacggcggct ggcggcgcgg caacgtggtg ccgctgaagg agaacgccga taaggcggtg
      541 gccgatacgc cctcgatcga gcacgtgctg gtggtgcagc gcgtcgggcc ggagcgcgcg
      601 ccgatccaga tgaagcaggg ccgcgatctc tggtggcacg agcagatagg caaagtgcag
      661 ggcaactacg tggagccgga gcagctcgac gccgagcacc cgctctatat cctctacacc
      721 tcgggtacga ccggcaagcc caagggcgtg ctgcacacca ccggcggcta cctggtcgga
      781 acctactcgt cgtgcaagtg ggtcttcgac atgaaggata gcgacatctt ctggtgtacg
      841 gcggatatcg gctgggtgac cgggcatagc tacgtcgtgt atggcccgct ggcctacggc
      901 gtctcccagg tgatgtttga gggcacgccc gactaccccg acaaagaccg cttctgggac
      961 atcatcgagc gacaccgcgt gaccgtgctc tacaccgcgc cgaccgccat ccgcggcttc
     1021 atgcgctggg gcaggcagta cccggagatg catgacctct ccagcctgcg gctgctcggc
     1081 acggtgggcg agccgatcaa tccggaggcc tgggtctggt acaacgacgt catcggccac
     1141 ggccgctgcc cggtagtcga tacctggtgg cagaccgaga ccggggcgat catgatctcc
     1201 gatctgcccg gcgtcacggt cgccaagcca ggctcggcgg ggccgccgat gccggggatc
     1261 gtgcccgacg tcctcgacaa cgatggcaac ccggtgccgg ccggtcacgg tggtttcctg
     1321 gtcatcaagc ggccttggcc gtcgatgttc cgcaccatct ggggcgactg ggaccgctac
     1381 gtggagacct acttcggcaa gtacggcccg aagatctacg tcaccggcga cggcgccaag
     1441 cgcgacgagg agggctatta ctggctgatg ggccgtgtcg acgacgtgct gaacgtggct
     1501 gggcaccggc tggggaccat ggagctggag agcgcgctgg tcagccaccc ctcggtggcc
     1561 gaggcggccg tcatcggcgt gagcgacgcg ctcaagggtc aggcggtggc ggccttcgtc
     1621 acgccgcggt tgggggtcga gccgagcagc aagctctctg aggagctgcg ccggcacgtg
     1681 gccgagaaga tcggcccgat cgcgcggccc gaccgcatct acttcaccgc cgaactgccg
     1741 aagacacgca gcgccaagat catgcgccgg ctgctgcgcg acgtcgccga gggtcgcgtg
     1801 atgggcgaca ccaccacact gaccgacgcg acggtgatcg aggagatcaa gcgcaactac
     1861 gagcagaacg aggggggcga gtagccctca tcccccagcc cccttctccc aatcctggga
     1921 gaaggggggg tacggtatgg ttggggcgtt gtgctccctc tccagcactg gctcggaccg
     1981 gcgagggctg gggtgagggt gcgccggagc gctaccggct cacgtccggc gggccttccc
     2041 aggagggggg accgcccgtg gtgaagctga gatgggccat gtcgtggccc gcctgggcgc
     2101 cgtgccagtg gcgctggccg ggcggggtgt agaccacgtc gccggtgctg aaggtcattg
     2161 tctcggactc ggagccggcg cggccctggc cctcgacgat caccagcacc tgttcgccgg
     2221 ggtgggtgtg ccagttggtg acggcgccgt cgcggaaacg gaccagcgac aacgccgcgc
     2281 cttgctcctg ctgggccggc agtaggcgct ccagccgggt cggtccggtg aacgtcttgc
     2341 cctcttcgga ggcccagtcg cctctcgaga tcaccttcac gggatgctcc tctcggtagg
     2401 acgattgtcg gagagagctc gcgcaaggat tgttcccggc gcgcgggtat tattgcgcgg
     2461 ttgaggctat gcgcggaggg tggagaggaa gagcatgaat atggagcgac aggtgacagg
     2521 cgcgcccggc agcctgttgg agacgctggc gatgacgatc gtcgagcgcg tgccggagcg
     2581 tgtggtggtg acgatgccgg tcaccagcgc gcaccaccaa ccgctgggct acctgcacgg
     2641 cggcgccagc gtggcgctgg ccgagacagc ggccagcgtc gccgccaatc tcaactgtgg
     2701 cccggacaag gtcgccttcg ggcaggagat caatgccaat cacctgcgtc cgattcgtga
     2761 cggcctgctg accgccaccg cgacgccgct gcacatcggg cgcacctcgc aggtctggga
     2821 cgtcaagatc cgcgacgagc aggagcgtct ggtctgcgtg tcgcgctgca ctctggcggc
     2881 ggtaccgcgc aatcgccagt agactggctc agttcctggt cgcgtggccg gcgggcttgc
     2941 ggctgggagg cgcgctgagc acccagggga cggtcacggc gaagcgcaac agcattaccg
     3001 gctggtcggc gaaggcgtcg cgcatcgagg gtgtcatcgg cacagtggcc ccgttgaacc
     3061 agatggcggc acggtcgccc atcgggatcg gccgccacgg gtcgaccccc tccagcactt
     3121 cacccacgaa ggggaagtgc ccgatgtagt ggtgccagcc ggggccgacc tcggccacct
     3181 cctgcgtctc cgcctcgcgc gtgcgatcca cgccgagttg ttccaacacc gcgaggagtt
     3241 cctcgggata gacgctctcg cgggcagcga cgaagttgcg gcagtagcgg cagcggcaac
     3301 tgcagggcag cgatgcgctg ctgcgatagc ggtctttggt ggcctccgga tcgacgcgca
     3361 gcgaccactc gccgatcacg atctctctca cgggccacac ctctccaccg gttgggcagc
     3421 gagcacggtt ggacccggcc ggcggggacg ggaacacaaa tggaccgggg acatcacgac
     3481 gcacctcgtc cgcgcgctga ggaacgtcac caggcgcaca ggagccgccc acgagcgaca
     3541 cggcgtgttc ccgccaagtc tacccgccgc cctcgcgcgc ccgccagcga gcgggccggc
     3601 aagagtcctg ggttcagtcg gccggctccg gctttgggtc ttcctgcttg gcggagccgc
     3661 gcgcgccgat ccaatcgggg atctgcacca gggcgagctg gagcacgatc gccacgaccg
     3721 ccccgaccac gatctgctga ctgagcagca ggctccagtc ccggctgagc ttcgagaaga
     3781 ggggatcgcc catcagcggc agcccgacgc cgagcatcac cgccagcccc gcgaccatgt
     3841 agttgcgctc ggtcggcgcc atcttggcca gcaggtcgat gcccaccacg atcagggatc
     3901 cggcggccgg caggaagatg ccaccgacta cgggatcggg cgtggccgcc agcagcgcgc
     3961 ccagcttcgg cacgaaggcc agcaccagaa agatcgcgcc ggcgtaggtg accgcgtggc
     4021 gcgtgccgat cttggtcagg cgcagcagcc cgacgttctg cgcgtaggcg gtggtggcga
     4081 agccgccgaa gagggccgag accatcgagc cagccgccgc gccggcgatg ccgcggttga
     4141 gccgctggtc ggtgatctcg gtgccgacga tctcggcggc ggcg
//
LOCUS       DASUBI010000115         6634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_244941, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000115 DASUBI010000000
VERSION     DASUBI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244941"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFU72_02215"
     CDS             <1..1337
                     /locus_tag="VFU72_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5422329.1"
                     /translation="ADGNYTFDDVPPGAALAVDSPDFAPWSGAIEQRTQVDVALRPDV
                     LTGRITDDKGAAVADATVVVGQQRAVTGADGSYRLEGAPASGQAVIKAPGYQVTTASL
                     NPELKLDARLTPIQVKALYAGAGTASDPARLDELIQIIDKTEANALVIDLKDSSGTVY
                     YDTQVPLAREIGAVDPSFDPKQIVDTLHQHHIYAIARIVVFEDPILAEKRPELAIHDS
                     AAGGLWRTWNGLAWVNAHKTEVWDYNIALAKEAVAFGFDEIQFDYIRFPSDGPLDRAD
                     YGVPQTAETREEAITGFLHMAYDAIAPTRAYLAADVFGLTMWATDDSDIGQNLEAVAP
                     YLDYICPMVYPSHFYAGSMGFDIPNDHPNEVILWSLEAGEKRVPQDKNKLRPWLQDFS
                     LGEGIAYGPDEVRKQIDAANDFGASGWMLWNAANVYHDTALKPQAASAGAGG"
     gene            complement(1564..2256)
                     /locus_tag="VFU72_02220"
     CDS             complement(1564..2256)
                     /locus_tag="VFU72_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU5422330.1"
                     /translation="MRLYVMQLGLGGGGSPVPGYLIQTDDGQNVLVDTGYPPQDYIGP
                     EARLPESDFVVNRLREIGLSPDDIDYVVATHLDVDHAGSHDLFPKAQFIVQDEHLSAA
                     REMPRFQGIRAHWEHPSLNYRTVDGDTELLPGIELIESSGHVPGHQSVLVRLPQTGPV
                     LLAIDAIPRDFTGYAPEERPTGQYDNDVEGTRRSTRKLVDLAEREGVAFTIRGHDADQ
                     WKQLKKSPEYYE"
     gene            2532..2996
                     /locus_tag="VFU72_02225"
     CDS             2532..2996
                     /locus_tag="VFU72_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5422331.1"
                     /translation="MLIVRRRSGRETSRIQQEMEELFRAMVVGSPSLARTSFGRWRPR
                     IEVYESDGALVVTAEVAGICEQDLSVVVDDSVLRISGVRPNPAKDHKRTYYEMGIPYG
                     PFEAEIFLPFSVVLDAVEAAYDNGFLRVTLPRPQATRIVPRATTTSTSKQER"
     gene            3003..5549
                     /gene="lon"
                     /locus_tag="VFU72_02230"
     CDS             3003..5549
                     /gene="lon"
                     /locus_tag="VFU72_02230"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5422332.1"
                     /translation="MVEKFTIADEPQEEREAREEEPLEPERAEQPGEETEPQTPEVLP
                     ILPLKGTVVFPMTLVPLAAGQPRSLRLIDDVVSGNRLVGLVLQKDPEQKTAGPGDTYT
                     IGTVASIHQMMRVPDGTVRLAVQGLRRIKILDWVGEEPFLTARVEDMPVEVEDSVEVK
                     ALMRNVVDLFQRMVALVSNLPEELVTAALNVEDPMHLVYLIASNLRMEPEEKQELLEL
                     DSARAKLQKLSAYLSKELDLLELGKKIQKDVQEEVGKTQREFYLREQLKAIQKELGET
                     NEQEAEATELRAKIDEAGMPEEAEREARRELERLSKLPPAAAEYGMIRTYLDWMVSLP
                     WNHSTEGVIDIAHTRQVLDEDHYDLEQLKERILEYLAVRKLRKEQAGEEAEEGREPIL
                     CFVGPPGVGKTSLAQSIARALGREFTRMSLGGVRDEAEIRGHRRTYIGAMPGRIIQAI
                     RRAGANDPVFVLDEIDKVGADWRGDPSSALLEVLDPEQNDTFRDHYLDVPFDLSKVMF
                     IATANVLDTIPPPLRDRMEILTLSGYTDDEKLHIARRYLLPKQLKRHALKPESIQLGD
                     DAILYLTQHYTREAGVRNLEREIATIARKVATQIAEGKDVSPVVGVEQVREYLGKPRF
                     FYEMLAERTAEPGVAVGVGVTAVGGDIMFIEATRMPGRGSLNVTGQLGDVMKESAQAA
                     LSFVRSRARDLGIASDFFKDTDIHLHVPAGAIPKDGPSAGITMTTALVSLLTGEPVAD
                     EVAMTGEITLRGQVLPVGGIKEKALAAGRAGITTFILPKRNEPDLDDLPPSLKENMRF
                     VLAETMEDVLRVALPGAFAAQASPHAVAREGGNEHHAVAAAARERGEKRG"
     gene            complement(5619..6368)
                     /locus_tag="VFU72_02235"
     CDS             complement(5619..6368)
                     /locus_tag="VFU72_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5422333.1"
                     /translation="MRKPLIGITPSPATDEHGTRYALSPNYTEAVEAAGGVPVILPPQ
                     QGNVDELLDALDGVILSGGGDIRPTIYGDTEQHPATYGIHDLRDQFELELARKAIARD
                     MPLLAICRGIQVLNVALGGTLIQDIADQHSDQIEHRQNRGEIPAAQPSHAVTAAPGSL
                     LARVYGATELQTNSFHHQALREVSPELRAIASAPDGTVESVEHPGCRWLLGVQWHPEM
                     MFRAHSEHLAPFQALVEAARAAQPAQAAAAD"
BASE COUNT         1153 a         2116 c         2344 g         1021 t
ORIGIN      
        1 gcgcggacgg caattacacc ttcgacgacg tgccgcccgg tgccgcgctc gccgtcgact
       61 cacccgactt cgctccctgg agcggcgcga tcgagcagcg cacgcaggtc gacgtggcgc
      121 tgcggccgga cgtgctcacc ggccgcatca ccgacgacaa gggagcagcg gtggcggacg
      181 ccacggtggt cgtcggtcag cagcgggccg tcaccggtgc ggacggcagc taccggttgg
      241 agggggcgcc ggcgagcggc caggcggtga tcaaggcacc gggctaccag gtgacgaccg
      301 ccagtctgaa cccggagctg aagctcgacg ccaggctgac gccgatccag gtcaaggcgc
      361 tctacgcggg cgccggcacc gccagcgatc cggccaggct cgacgagctg atccagatca
      421 tcgacaagac cgaggccaac gcgctggtga tcgatctcaa ggacagcagc ggtaccgtct
      481 actacgacac gcaggtgccg ctggcgcgag agatcggcgc ggtggatccg tcgttcgatc
      541 ccaaacagat cgtcgacacg ttgcaccagc accacatcta cgccatcgcg cgtatcgtcg
      601 tcttcgagga cccgatcttg gccgagaagc gtcccgagct ggcgatccac gactccgccg
      661 ccggcggtct ctggcgcacc tggaacggac tggcctgggt caacgcgcat aagacggagg
      721 tctgggacta caacatcgcg ctggccaagg aggccgtcgc cttcggcttc gacgagatcc
      781 agttcgatta catccgcttc ccctcggacg gcccgctcga tcgcgccgac tacggcgtgc
      841 cccaaacggc cgagacgcgc gaggaagcga tcaccggctt cttgcacatg gcctacgacg
      901 cgatcgcccc gaccagggcc tatctcgcgg ccgatgtctt tggactgacg atgtgggcca
      961 ccgacgacag cgacatcggc cagaatctgg aggcggtggc gccctacctg gactacatct
     1021 gccccatggt ctacccatca cacttctacg ccggctcgat gggcttcgac atccccaacg
     1081 accaccccaa cgaggtgatc ctctggagcc ttgaagcggg cgagaagcgg gtgccacagg
     1141 acaagaacaa gttgcggccc tggctgcagg atttcagctt gggcgagggg atcgcctatg
     1201 gaccggacga ggtgcgcaag cagatcgacg cggccaacga cttcggcgcc agcggctgga
     1261 tgctctggaa cgcggccaac gtctaccacg acacggcgct gaagccgcag gcggcgagtg
     1321 ccggcgcggg cggttgatca cgtggtatcg ggtgggacag cggcagtctg accgctgcca
     1381 atgacacgat aggcgcgatg cgtaggggca gacccccgtg tctgccctgt ctcccctccg
     1441 acatggcaac gggaacatgg ggcaacgccg gcggcggcga tgaagtcgga tgccggggtg
     1501 gcgagggcag acacggggtc tgcccctaca atggagagcg caaccgcccc ggtagaggac
     1561 agcctactcg taatactccg gcgacttctt gagctgcttc cattggtcgg cgtcgtggcc
     1621 gcggatcgtg aaggcgacgc cctcgcgctc ggctaggtcg accagcttgc gcgtgctgcg
     1681 gcgggtgcct tccacgtcgt tgtcgtactg gccagtcggg cgctcctcgg gcgcgtagcc
     1741 ggtgaagtcg cgcgggatag cgtcgatcgc cagcagcacc ggcccggtct gaggcaggcg
     1801 caccagcacc gactggtgcc ccggcacgtg gccgctcgac tcgatcagct cgatgccggg
     1861 gagcagctcg gtgtcgccgt cgacggtgcg atagttgagg ctggggtgct cccagtgcgc
     1921 gcgaatcccc tggaagcgcg gcatctcgcg ggcggcgctg agatgctcat cctggacgat
     1981 gaattgagcc ttggggaaga ggtcgtggct gccggcgtga tcgacatcga ggtgcgtggc
     2041 gaccacgtag tcgatatcgt cgggagaaag cccgatctcg cgcagccggt tgacgacgaa
     2101 gtcggactcc ggcaggcgcg cctcggggcc gatgtaatcc tgcggcgggt agccggtatc
     2161 gaccagcacg ttctgcccgt cgtccgtctg gatcagatag ccgggcaccg gcgagccgcc
     2221 gccgccgagc ccaagctgca tcacgtacag gcgcacgctt cacctcctga tggaacgaac
     2281 tcatcgaccg gcgcatcttc gcattgccga ccccggttcg caagcgcatc cacctatcgg
     2341 cacgcccgca ccggtctgat ataactcgaa tcaaccgacc tgatttataa gttgcagttg
     2401 tcctgagcgg gtgatatact ctgccggaat cctggcgcgg ggcaccggca tacatcatgc
     2461 aggacaggac ggacgccagg acggaagcgg tcccgtgcgg atcgcacggt acgcgcgaag
     2521 gaagtgaatt gatgctgatc gtgcgaaggc gaagcggccg ggagacgagc cggattcagc
     2581 aagagatgga agagctgttt cgcgcgatgg tggtgggatc accatccctc gcacggacga
     2641 gcttcggccg ctggcggccg cgcatcgagg tctacgaatc cgatggggct ctggtcgtga
     2701 cggcggaggt ggcgggtatc tgcgagcagg atctgagcgt cgtggtcgac gactcggtgc
     2761 tccgcatcag cggcgtgcgc ccgaaccccg ctaaggatca caagcgcacc tactacgaga
     2821 tgggaatccc ctacgggccg ttcgaggcgg agatctttct ccccttctcg gtcgtgctcg
     2881 acgcggtcga ggcggcctat gacaacggct tcctgcgagt gacgctgccg cgcccacagg
     2941 cgacgaggat cgtgccacgg gcgaccacga cgtcgacgag caagcaggag aggtaacaat
     3001 caatggtcga gaaattcacg attgcggacg agcctcagga ggagcgcgaa gcgcgcgagg
     3061 aggagcccct ggagcccgag cgcgccgagc agcccggcga agagaccgag ccgcagacgc
     3121 ccgaggtgct gccgatcctg ccgctcaagg gaaccgtcgt ctttccgatg acgctggtgc
     3181 cgctggccgc cggccagcca cgctcgctgc ggctgatcga cgacgtcgtc tcgggcaacc
     3241 ggctggtcgg cctcgtgctg cagaaggacc cggaacagaa gaccgccgga cccggcgaca
     3301 cctacaccat cggcaccgtg gcgagcatcc accagatgat gcgcgtgccc gacggcaccg
     3361 tgcgcctggc ggtgcagggc ctgcggcgca tcaagatcct ggactgggtc ggtgaggagc
     3421 cgttcctgac ggcgcgtgtt gaggatatgc cggtcgaggt ggaggattcg gtcgaggtca
     3481 aggcgctgat gcgcaatgtc gtcgacctct tccagcgcat ggtggcgctg gtctccaact
     3541 tgcccgagga gctggtaacc gcggcgttga acgtcgagga tccgatgcac ctcgtctacc
     3601 tgatcgccag caacctgcgc atggagccgg aggagaagca ggagctgctg gagcttgact
     3661 cggcgcgagc caagctgcag aagctgagcg cttacctcag caaggagctg gacctgctcg
     3721 agctgggcaa gaagatccag aaggatgtcc aggaggaggt cggcaagacc cagcgcgagt
     3781 tctacctgcg cgagcagttg aaggcgatcc agaaggagct gggcgagacc aacgagcagg
     3841 aggccgaagc caccgagctg cgcgccaaga tcgacgaggc aggcatgccg gaggaggccg
     3901 agcgcgaggc ccggcgcgag ctggagcggc tgagcaagct gccgccggcc gccgccgagt
     3961 acggcatgat ccgcacctac ctcgactgga tggtcagcct gccctggaac catagcaccg
     4021 agggcgtgat cgacatcgcg cacacgcgtc aggtgctcga cgaggaccac tacgacctgg
     4081 agcagctcaa ggagcgcatc ctggagtacc tcgccgtgcg caagctgcgc aaggagcagg
     4141 cgggcgagga ggccgaggag ggacgtgagc cgatcctctg cttcgtcggg ccgccgggtg
     4201 tcggcaagac cagcctggca cagtcgatcg cccgggcgct cgggcgcgag ttcacgcgca
     4261 tgtcgctggg cggcgtgcgc gacgaggccg agattcgcgg ccaccggcgc acctacatcg
     4321 gtgccatgcc ggggcgcatc atccaggcta ttcgccgggc cggcgccaac gatccggtct
     4381 tcgtgctgga cgagatcgac aaggtcggcg ccgactggcg cggcgacccg tcgtcggcgc
     4441 tgctcgaggt gctcgatccg gagcagaatg acactttccg cgatcactac ctcgacgtgc
     4501 cgttcgatct gtcgaaggtc atgttcatcg ccacggccaa cgtgctcgat accatcccgc
     4561 cgcccctgcg cgaccggatg gagatcctga cgctgtcggg ctataccgac gatgagaagc
     4621 tgcacatcgc gcggcgctac ctgctgccga agcagctcaa gcgccacgcg ctcaagccgg
     4681 agtcgatcca gctcggcgac gacgcgatcc tctacctgac ccagcactac acgcgcgagg
     4741 cgggcgtccg taacctggag cgcgagatcg ccacgatcgc gcgcaaggtg gcgacgcaga
     4801 tcgccgaggg gaaggacgtc tcgccggtgg tcggcgtgga gcaggtgcgc gagtacctgg
     4861 gcaagccgcg gttcttctac gagatgctcg cggagcggac cgcggagccg ggtgtcgcgg
     4921 tcggcgtcgg ggtgaccgcg gtcggcggcg acatcatgtt catcgaggcg acgcgtatgc
     4981 ctgggcgcgg cagcctgaac gtgaccggtc agctcggcga cgtgatgaag gagtcggcgc
     5041 aggcggcgct cagcttcgtg cgctcgcgcg ctcgcgatct cgggatcgcg tcggacttct
     5101 tcaaggacac cgatattcac ctgcacgtcc cggcgggcgc gatccccaag gacgggccgt
     5161 cggccggtat caccatgacg accgcgttgg tgtcgctcct gacgggcgag ccagtggcgg
     5221 acgaagtggc catgaccggg gagatcacgt tgcgcggtca ggtgctgccg gtgggcggca
     5281 tcaaggagaa ggcgctggcg gccggacggg ccggcatcac gaccttcatc ctgcccaagc
     5341 gcaatgagcc ggacctggac gacctgccgc cctccttgaa ggagaacatg cgcttcgtcc
     5401 tggccgagac gatggaggac gtgctgcggg tggccctgcc cggcgccttc gccgcgcagg
     5461 cgagccccca cgccgtagct cgtgagggcg gcaacgagca ccacgcggtg gccgcggctg
     5521 cccgcgagcg cggcgagaag cgcggctaac ggttcaatcg ttaacccgtg cggccccagc
     5581 tcttgctggg gccgctttcg tttgtcgggt ttaccggctc agtcggccgc ggctgcctgc
     5641 gccggttggg cggcccgggc ggcttcgacc agggcctgga agggcgcgag atgctcgctg
     5701 tgggcgcgga acatcatctc ggggtgccac tgcacgccca gcagccagcg acagccgggg
     5761 tgctcgaccg actcgacggt cccgtcgggc gcgctggcga tggcgcggag ctcggggctg
     5821 acctcgcgca gcgcctggtg gtggaaggag ttggtctgca gctcggtggc gccgtagacg
     5881 cgcgccagca gcgagccggg cgcggcggtg acagcatggc tcggctgggc ggccgggatc
     5941 tcgccgcggt tctggcggtg ctcgatctgg tcgctgtgct ggtcagcgat gtcctgaatc
     6001 aaggtgccgc cgagggcgac attgagcacc tggatgccgc ggcagatcgc cagcagcggc
     6061 atgtcgcgcg cgatcgcctt gcgcgccaac tccagctcga actgatcgcg caggtcgtgg
     6121 atgccgtagg tggcggggtg ctgctcggtg tcgccgtaga tggtggggcg gatatcgccg
     6181 ccgccgctga ggataacgcc gtccagcgcg tcgagcagct catcgacgtt gccctgctgt
     6241 ggcggcagga tcaccgggac gccgccggcg gcctcgaccg cctcggtgta gttgggcgac
     6301 agcgcgtagc gcgtcccatg ctcgtctgtc gctggcgaag gcgtgatgcc gatgaggggt
     6361 ttgcgcatgc ggagatcctc tcgtgtcagg gtgactgccg gtgccgtcaa cactcgccgc
     6421 tgattattcg gcgcgcgaga gatccggtca agcacccctt gtcgggtgct gggcgagggt
     6481 gctatactct gacacgtcgt caaccggacg acgacagacg ccggttgggc gggcgagagc
     6541 ggctgccctt gacagtagac gactcgctga gtatactaat cggtgcgtct gtaagaggga
     6601 taactcctca gagccgcgag gctcgatgtg tagc
//
LOCUS       DASUBI010000116         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1304394, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000116 DASUBI010000000
VERSION     DASUBI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304394"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1876)
                     /locus_tag="VFU72_02240"
     CDS             complement(131..1876)
                     /locus_tag="VFU72_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5422334.1"
                     /translation="MLTNPDIPVIDTDVAIIGSGGAGFLCLLHLARAAPKLDVTIVSK
                     GAMGRSGCTRMVQGGYNAVLDPRDSLQLHFADTLKGGAFLNDQELAWTLVSDAPKIIH
                     ELETVAGCFFDRRPDGHIHQKPFAGQSFDRTVHRGDLTGIEIMGRLRDQLFRIKPREL
                     EDVRALDLIFDARGELAGVTLLDVRTGAAALLRARVVVVATGASATMYKVSAPAREKT
                     GDGQAMCWRASLPFRDMEMLQFHPTGLLAGDSRLSGAVLEEGLRGSGAYLFNALGERY
                     MARYDPEHMERATRDVVSRASYLEIMAGRGTAAGGVLIDISHLGAENVERLFPGMVER
                     TRLIGADLARGPVDVSPTAHFHMGGVVIDRDCRTELPGLLVAGEDSGGTHGANRLGGN
                     GVAESTVFGARAGDSAACIAPERPLRDPDPAQVAASLARAYAPLGREGVGANPFAVTR
                     ALKELMWQDCGVVRTREGLLRARARLRELAEEAGRIGVPGPAAANYGWQEALDVVNQI
                     TVAELVVEAALLREESRGAHYRADFPARDDARWLRAIIQRRGADGECETSYQPVELSR
                     LRPEAAIEAEAGGRA"
     gene            complement(1866..2618)
                     /locus_tag="VFU72_02245"
     CDS             complement(1866..2618)
                     /locus_tag="VFU72_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU5422335.1"
                     /translation="MPTPASVSSKPVAPAGSTFVTVHVARSDPASPGTFRVARFEPMM
                     LLDLLLAIQREHDPALGFRYSCRVGMCGTCTLRMDGRSVLACQTRLRPDQHELRLDPL
                     AGLPLVRDLIVDTTPFWQEWARVTPYLVPRPDLTEPAVIPPDSSERRTIDPHLDCIGC
                     AACYSGCGIADYRRDFLGPAALSRALVLIADSRDARRVERLRQVSGADGTLRCRYIHA
                     CTAACPKGLDPAGAIRRLRRWRLEVGGLSDAD"
     gene            complement(2590..3222)
                     /locus_tag="VFU72_02250"
     CDS             complement(2590..3222)
                     /locus_tag="VFU72_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5422336.1"
                     /translation="MATDARESLPRDAQGVIRLTTPLVEATTRALRVGDQLLVDGHIW
                     GIRDATLIQIFDRGETPSVDLSGAILLHVAPSVKRRPDGGYDPVSVGTTTSSRMERFT
                     AGCLGQLGARAIVGKGGFSDAALPVLQRLGGVYLAIVGGAASPETLQVEEIEAVYYKE
                     LMPECLWQFRVKGFGPLFVTMDTHGESAYHDVREQAGKNLQDAYARLGIE"
     gene            complement(3212..4120)
                     /locus_tag="VFU72_02255"
     CDS             complement(3212..4120)
                     /locus_tag="VFU72_02255"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU5422337.1"
                     /translation="MTSTTSQTLTGEQLYETVREVSCELYKRSLTDLPPDVRRSLAGA
                     LEHEEGRHARMMLQIMRHAVETSDKTGLIVCQDTGIPVYFVGIGTELTLNGAKLLQAL
                     KDGIERATARYNLRSSIVHPITRTNNQTSTGREVPVMHVDFLDGATYLDFELVPKGSG
                     SENMSFVKMLTPADGLNGVKKFVLDSVVAAGANPCPPTIVGVGLGGSFDKCAELAKRA
                     IKRPVGQSHPEPEIAALERDLLEAINLTGIGPQGLGGDTTSLAVHIEYAWTHITMNPV
                     AVNIQCWRGERARARVYGDGRVEYGY"
BASE COUNT          588 a         1463 c         1553 g          584 t
ORIGIN      
        1 ccgaagaagc gcgcgtcgag gtaggcgccc gagccgctgc gcgccgcccc caccccgatc
       61 agcaccccga acccgagcac gaccaggagc acgacgctca gcgccgccgc gaagaggagt
      121 tgcacgacgg tcacgcgcgc cctcccgcct ctgcctcaat cgccgcctcg ggtcgcagcc
      181 ggctcagctc gaccggctgg tagctagtct cgcactcgcc gtcggcgcca cgccgctgga
      241 tgatggcgcg cagccagcgc gcgtcgtcgc gcgcggggaa gtcggcgcgg tagtgggcgc
      301 cgcgtgattc ctcgcgcagc agcgctgcct cgaccaccag ctcagccacg gtgatctggt
      361 tgactacgtc gagcgcctcc tgccagccgt agttggcggc ggccgggccg ggcacgccga
      421 ttcgcccggc ctcctccgcc agctcgcgca gccgggcgcg ggcgcgcaag agcccctcgc
      481 gcgtgcgcac cacgccgcag tcctgccaca tcagctcctt gagcgcgcgg gtgacggcga
      541 agggattggc gcccaccccc tcgcggccga gcggagcgta ggcgcgcgcc agcgaggcgg
      601 cgacctgcgc cgggtcgggg tcacgcagcg ggcgctcggg ggcgatgcac gccgcggaat
      661 cgccggcccg cgcgccgaag acggtggact cggccacgcc gttgccgccc agccggttgg
      721 cgccgtgcgt gccgccggaa tcttcgccgg ccaccagcag ccctggcagc tccgtgcggc
      781 agtcgcggtc gatcaccacg ccgcccatgt ggaagtgggc cgtcggcgag acgtcgaccg
      841 ggccgcgcgc caggtccgcg ccgatcaggc gggtgcgctc gaccatgccg gggaacaggc
      901 gctccacgtt ctcggcgccc aggtgcgaga tgtcgatcag cacgccgccg gccgccgtgc
      961 cgcgcccggc catgatctcc aggtagctgg cgcgcgagac gacgtcgcgg gtcgcgcgct
     1021 ccatgtgctc ggggtcgtag cgcgccatgt agcgctcgcc gagcgcgttg aagagatagg
     1081 cgcccgagcc gcgcaggccc tcctccagca ccgcgccgga gaggcgcgag tcgccggcca
     1141 ggagcccggt cgggtggaac tgcagcatct ccatgtcgcg gaaggggagt gaagcgcgcc
     1201 agcacatcgc ctggccgtcg ccggtcttct cgcgcgcggg ggccgagacc ttgtacatgg
     1261 tggccgaggc gccggtcgcc accaccacca cgcgcgcccg cagcaacgcc gccgcgccgg
     1321 tgcgcacgtc gagcagcgtc accccggcca gctcgccccg cgcgtcgaag atcaggtcga
     1381 gcgcgcgcac gtcctccagc tcgcgcggct tgatgcggaa gagctgatcg cgcagccggc
     1441 ccatgatctc gatgccggtc aggtcgcccc ggtgcacggt gcggtcgaac gactgcccgg
     1501 cgaagggctt ctggtggatg tggccgtcgg ggcggcggtc gaagaaacag ccggccaccg
     1561 tctccagctc gtggatgatc ttgggcgcgt ccgagaccag cgtccaggcc agctcctggt
     1621 cgttgaggaa ggcgcccccc ttgagggtgt cggcgaagtg cagctgcagc gagtcgcgcg
     1681 ggtcgagcac ggcgttgtag ccgccctgca ccatgcgggt gcagccggag cggcccatgg
     1741 cgcccttgct gacgatggtg acgtcgagct tgggcgcggc gcgcgccagg tgcagcaggc
     1801 agagaaagcc ggcgccgccg ctgccgatga tcgccacgtc ggtgtcgatg accgggatgt
     1861 cggggttagt cagcatcgga cagccccccc acctccagcc gccagcggcg cagccggcgg
     1921 atcgcgccgg ccgggtcgag ccccttgggg caggcggcgg tgcaggcgtg gatgtagcgg
     1981 cagcgcaggg tgccgtcggc gccgctcacc tggcgcagcc gctcgacgcg gcgcgcgtcg
     2041 cggctgtcgg cgatcagcac cagggcgcgg ctgagcgccg ccgggccgag gaagtcgcgg
     2101 cggtagtcgg cgataccgca gccggagtag caagcggcgc agccgataca gtcgagatgc
     2161 gggtcgatgg tgcgccgctc gctcgaatcg ggcgggatca ccgccggctc ggtcagatcg
     2221 ggcctgggga ccaggtaggg cgtgacccgc gcccactcct gccagaaggg ggtggtgtcg
     2281 acgatcaggt cgcgcaccag tggcagcccg gccagcgggt cgagccgcag ctcgtgctgg
     2341 tcggggcgca gccgcgtctg gcaggccagc accgagcgcc cgtccatgcg taacgtgcag
     2401 gtgccgcaca tgccgacgcg gcacgagtag cggaagccga gcgcggggtc gtgctcgcgc
     2461 tggatggcca agagcaggtc gagcagcatc atcggctcga agcgggccac gcgaaaggtg
     2521 ccggggctgg ccgggtcgct gcgcgccacg tggacggtga cgaaggtgct cccggccggg
     2581 gcgaccggct tactcgatac cgaggcgggc gtaggcatcc tgcaggttct ttcccgcttg
     2641 ctcgcgcacg tcgtggtagg cggactcgcc gtgcgtgtcc atggtgacga agagcgggcc
     2701 gaagcccttg acccggaact gccagaggca ctcgggcatc agctccttgt aatacaccgc
     2761 ctcgatctcc tccacctgga gcgtctccgg gctggccgcg ccgccgacga tcgccaggta
     2821 gaccccgccc agccgctgga gcaccggcag cgcggcgtcg ctgaaaccgc ccttgccgac
     2881 gatggcccgc gcgcccagct ggcccaggca gcccgcggtg aagcgctcca tgcgcgagga
     2941 ggtggtggtg ccgaccgaga ccgggtcgta gccgccgtcc gggcgccgct tcacgctggg
     3001 cgccacatgc agcaggatgg cgccgcttag gtcgacgctc ggcgtctcgc cgcggtcgaa
     3061 gatctggatc agcgtggcgt cgcggatacc ccagatgtgg ccgtcgacca gcagctggtc
     3121 gccgacccgc agcgcccgcg tggtcgcctc aacgagcggg gtggtcaggc ggatgacgcc
     3181 ctgggcatcg cgcggcagag actcgcgggc atcagtagcc atactcgacc ctcccgtcgc
     3241 cgtagacgcg agcgcgggcg cgctcgccgc gccagcactg gatgttgacc gccaccgggt
     3301 tcatggtgat gtgcgtccag gcgtactcga tgtggaccgc cagcgaggtg gtgtcgccgc
     3361 ccagcccctg cgggccgatg ccggtcaggt tgatcgcctc cagcaggtcg cgctccagcg
     3421 cggcgatctc cggctcggga tgcgactggc cgaccgggcg cttgatggcg cgtttggcca
     3481 actcggcgca cttgtcgaat gagccgccca gcccgacacc gacgatggtc ggcgggcaag
     3541 ggttggcccc ggccgccacg accgagtcga gcacgaactt cttgacgccg ttgaggccgt
     3601 cggccggggt cagcatcttg acgaagctca tgttctcgga gccggagccc ttgggaacga
     3661 gctcgaagtc gaggtacgtg gcgccgtcga ggaagtcgac gtgcatcacc ggcacctcgc
     3721 gcccggtgct ggtctggttg ttcgtgcggg tgatcgggtg gacgatgctg gagcgcaggt
     3781 tgtagcgtgc cgtggcgcgc tcgataccgt ccttgagcgc ctgcaggagc ttggcgccgt
     3841 tgagcgtcag ctcggtgccg atgccgacga agtagaccgg gatgccggtg tcctggcaaa
     3901 cgatcaggcc ggtcttatcc gaggtctcga cggcgtggcg catgatctgg agcatcatgc
     3961 gggcgtggcg cccttcttcg tgctccagcg cgcccgcgag cgagcggcgc acgtcgggcg
     4021 gcaggtcggt cagcgagcgc ttgtagagct cgcaggagac ctcgcgcacc gtctcgtaga
     4081 gctgctcgcc ggtcagggtt tgggaagtgg tgctcgtcac gggccgctcc tcgctgttcg
     4141 acgcgttgtg ggatcagccg gccacaatcc cggtcagccc ggcgcctg
//
LOCUS       DASUBI010000117         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1345161, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000117 DASUBI010000000
VERSION     DASUBI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345161"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFU72_02260"
     CDS             <1..234
                     /locus_tag="VFU72_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422338.1"
                     /translation="AAVPGTEGRALGLWMIVNSGLVPFGSLAIGAIAEPAGVRGALGG
                     AGVICALCGLAAVYVVRRTTRPKKLTATAEPMS"
     gene            complement(1582..2727)
                     /locus_tag="VFU72_02265"
     CDS             complement(1582..2727)
                     /locus_tag="VFU72_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU5422339.1"
                     /translation="MQVTRVETLWPSSIEPASFFFVQVHTDEGYVGLGQAPDPQRTIP
                     AVEEWSRRFLLGQDPLAIARFWDRAFTAARAHGYAGAELRALSALDIALWDIAGQVAG
                     QPIYQLLGGKVRDRIRVYNTCSHYRDYNDRLLVRQDPLRLVEELLAEGITAFKFSMFD
                     ALADATLGTYLAHDDLVRAVEPIRRVHEAYGTGIEIGIEGHARWNLPMATRIAQYLER
                     FHIMWLEDLLPPDNPAQLGQLRARTRLLLGGSELLFTRWHFLPLLQAGGTDIVIADIT
                     WCGGISELARLAALAATFDLPLAPHDHTGPVGLFATAHVMAQAPNGLVMETTRAFYRT
                     YYPDLVDPAPVIRDGHLALPEGPGLGTRLRPAVWQRPDLVVHVVAPA"
     gene            complement(2824..>3430)
                     /locus_tag="VFU72_02270"
     CDS             complement(2824..>3430)
                     /locus_tag="VFU72_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422340.1"
                     /translation="VGTVIVHVGGESLIADIGRGRYSKAYWGPERYDLLVKSSRGHSV
                     PIVNGYAQGGGRDYAARVLEHAVGAAADGEDRLVLALEGAYPAEAGVAALTRTIALHR
                     RPPRGAVTLADCVRFAYGPGHLTSVITTFAPVAVGDGQVRICGAHAALQIDYDAGTIT
                     VEVVTEPAVDFPAGPQAVNRILFTLRVPAEEASIRLAIVPE"
BASE COUNT          585 a         1125 c         1169 g          551 t
ORIGIN      
        1 gccgccgtgc ccggcacgga gggccgcgcg ctcggcctct ggatgatcgt caactctggc
       61 ctggtgccgt tcggcagtct ggcaatcggc gcgatcgccg aaccagccgg cgtgcgcggc
      121 gcgctcggcg gcgcgggcgt catctgcgcc ctttgcgggc tggccgccgt ctacgtggtc
      181 cggcgcacaa cccgcccgaa gaaactgacc gcgacggcgg agccgatgtc atagcggtta
      241 caggttcttc cggtaagcac gatgtttgcc tgctgcaact tgcaacctgt caacctgcaa
      301 cccagcaacc atgccatagc gtgacctgtc aggtgccagg cattatatgt agccggattg
      361 tcactgccgc gccgggtgcg tgggtgaaga tgatgcggtt gactactcat cgctcgttgg
      421 acaaggccat ctctcggcgt gctcggccag gcgctcgtgc tcggtcgctg gcgctggctg
      481 gcggcgctga ccaccctcgc cagcgcctac cggcggaacg ccgcctcgtc gtagcccgcc
      541 ttccgccgac actccggcgc cgcatgggag gcctacgccg cgaccgtccc cgccttcctg
      601 ccgcgccgcc ggggaactgc atcgagacca agcggcggtg cttcagaaag gagtagccaa
      661 tgaccctcct acggcacgcc gttcgcacga tgcgccggct cgacccggcc gtggcgagtg
      721 ccgcgctctt tcttcgcagc gagctgcgcg acaggatcgc cgtgaggacg gacggcggta
      781 attcctcctg gtcggtcagg gcaggttgat acacgacgat ccagcccgcg tgtaagcgca
      841 gcgtacggct ccaggtgcag cgcctgagcg gaagagccgt tgtctggttc gcagaacgcc
      901 gaatatgcga acacatctgg tgcgcattgt gctctcagga taaggctagg ttagacggcg
      961 aggtacgatt ggtatcagga tagagtccgt ggccgactct acctggacat gggcgagcta
     1021 gccgagggag agcgcgccgt gcccggcggg gcgaggcatg ccccgccccg ccgggcggtc
     1081 aactgaggca gtggagcgtg gaggaactta cagaactttg cggacttgac ttggtgtcag
     1141 tgcgcatcgg cgaatttgta ggcctttgtc agtacaccag caaaactatc ggcacatttc
     1201 acgtcggcat aggagactag gccagatgac cgctgttaac tagccgcagc gcgcaactgg
     1261 tgcgctgcgg catccttgcg cggcacatcc atggattccc gtgcattctc tttcttcatc
     1321 tcgaccaagt cgaacgattg cgctgtctcc acaatgacat caggggtgta gttgtttacc
     1381 cgtagaagat cggaaaacgc ccgatcggta cgcgcacccg agccagagag ccgccgtcgg
     1441 tctccttgga gcggaagccg tagccggacg ccccaccaaa cgggatgtgc ccggctagga
     1501 cagcggcgct cgacgcacgc tcgctgcgcc tcgttgtcct ggccgggcgt gctgacgttc
     1561 aggcggctct gcaccgccag atcaggctgg cgcaacgacg tggaccacga ggtccgggcg
     1621 ctgccacacg gcgggccgca gtcgggtgcc aagcccgggc ccttctggca gggcaagatg
     1681 cccgtcgcgg atgaccggcg cgggatcgac cagatcgggg tagtaggtgc ggtagaaggc
     1741 gcgcgtcgtc tccatcacca acccattcgg cgcctgggcc atgacgtgcg ccgtggcgaa
     1801 gaggccgacc ggacccgtgt ggtcatgggg cgcgagcggc aggtcgaacg tcgccgccag
     1861 tgcggcgagg cgcgccagtt cggagatgcc accgcaccag gtgatgtcag cgatcacgat
     1921 gtcggtgccc ccggcctgga ggagcggtag gaaatgccag cgggtgaaga ggagttcgct
     1981 gccgccgagc agaaggcgtg tgcgggcgcg caactgtccg agttgcgcgg ggttatccgg
     2041 cggcagcagg tcctccagcc acatgatatg aaagcgctcc agatactggg cgatccgcgt
     2101 cgccatcggc agattccagc gggcgtggcc ctcgatcccg atctcgatcc ccgtgccgta
     2161 ggcctcgtgg acccggcgaa tcggctcgac cgcgcggacc aggtcgtcat gcgccaggta
     2221 ggtcccgagc gtggcgtcgg ccagggcatc gaacatcgag aacttgaagg ccgtgatccc
     2281 ctccgccagc agctcctcca ctagtcgcag cggatcctgc cggaccagca gccggtcgtt
     2341 gtagtcgcgg taatgtgaac aggtattgta gacgcggata cggtcgcgca ccttgccccc
     2401 gagcaactgg tagatgggct gcccggcgac ctgcccagcg atgtcccaga gggcgatatc
     2461 cagcgccgag agtgcccgca gttccgcgcc ggcatagccg tgcgcgcgcg ctgcggtgaa
     2521 ggcccggtcc cagaatcgcg cgatggcgag cggatcttgg ccaaggagga agcgccggga
     2581 ccattcctcg acggccggga tcgtgcgctg gggatctggg gcttggccga ggccgacata
     2641 gccctcatcg gtatgcacct gcacgaaaaa gaaggaggcg ggttcgatgc tactgggcca
     2701 gagcgtctct acacgcgtca cttgcatcag atcctccttg cgcgaacccg gtccggcgcg
     2761 agcagcgcgc caccgaacca cgggcacgag tcgccactgg tatccgggtg cgagtcgcac
     2821 gcgtcactca ggaacgatcg ccagccggat gctcgcctcc tcagccggga cgcgcagcgt
     2881 gaagaggatg cggttgaccg cctgcggccc ggcggggaag tcaaccgctg gttccgtgac
     2941 gacctcgacc gtgatggtgc cggcgtcgta gtcgatttgg agggccgcat gtgcgccgca
     3001 gatacgcacc tggccgtcgc cgacggccac cggcgcgaag gtcgtgatga cgctggtgag
     3061 gtggccgggt ccatacgcga agcggacgca atcggccagc gtgaccgcgc cgcgcggcgg
     3121 ccggcggtgc aaggcgattg tccgcgtcag cgcggccacc cccgcctcgg caggataggc
     3181 gccctccagg gcgaggacca gccgatcctc cccgtcagcg gccgccccga ccgcgtgttc
     3241 cagcacacgc gcggcatagt cgcgcccgcc accttgcgcg tagccattga cgatcgggac
     3301 cgaatgacca cgggaggatt tgacgaggag gtcgtagcgc tccggccccc agtacgcctt
     3361 cgagtagcgc ccccgcccga tgtcagcaat cagcgactcg ccgccgacgt gcacgatcac
     3421 ggtgccgacg
//
LOCUS       DASUBI010000118         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_650420, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000118 DASUBI010000000
VERSION     DASUBI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650420"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFU72_02275"
     CDS             complement(<1..1273)
                     /locus_tag="VFU72_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422341.1"
                     /translation="MAARNVAARLAPLGIGRLGGERVSGAVQLVRAWWLILGVSTLLL
                     VVDPSLRVTFRHDALSFTIDTFTALVATMVAFVGLARYVFDRRPLDLAIAAAFSAVAV
                     SSLWFGLILPFKGHPFHGSASAALYGWLFTRFVVVVLLLLGLARRTPGRLPPRRAELW
                     LAGVVALVTAVDVGLWYGRGWLPRLLGRDAWAPLMFRRDPITIPILAGQTELGIVLES
                     VVALLYCWLALRLTAAVREGENAWLALSLITAAVAEVQFVFYPAPFHPAITTADFLWL
                     VSYLLLLVYLGSQFVRASSEARRQQVRTSALLALSQTPVVNRDPTLVSEAASRAARVV
                     APEATVAVELGGQADDGANEQRYPISADGTSYGWLVVTPLRRPLAHDTEAYLRIVANQ
                     TASLLRAIALYAELEEGAVRDERAQLARELHD"
     gene            complement(1532..2659)
                     /locus_tag="VFU72_02280"
     CDS             complement(1532..2659)
                     /locus_tag="VFU72_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEU5422342.1"
                     /translation="MQHRTYALFAAVHAALRRRQRRARLFGVFAAALVALSLVAGVQA
                     SGTPALSLSPTSLTFAAQDTGTTSAPQTITATNTGTASLFFNNVALTGADGLDFTISD
                     DQCIGTTLAVGASCTIAVTFSPTTTGTRSENVTFTDNAPDSPQSVPLTGGGTGTAAPL
                     AINDQFFSCANGVCDIGAGSNVFVNNFYTNSFLASGGAQPYTWSGQVPAGLTLRPSGL
                     LLGDPTILGTQTFNVTVTDAAGNTATGTFSLTVTNSPAPSPSGCQTGGTLKESLSGTT
                     LNGKTPRGQATADESKFSGCGGFSLLSVQVKDVNLPDGTVLWVTLDFKPVGTITLRGG
                     SGTMPTYNMGRFGVSRDQIRVYNSLPDLSGYTQILIGNAFS"
     gene            3007..>3784
                     /locus_tag="VFU72_02285"
     CDS             3007..>3784
                     /locus_tag="VFU72_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422343.1"
                     /translation="MSSRLERLDGRVVLGWLARAPFTLALLAVMLALGIATGSIRHQL
                     PGSGLLARVGFGLPALQHGRYYTLVTCIPFALYPWMLATITLLVVLLMLPYEVVAGTR
                     RAALVFLACQLGGYLLAALLVAWPLSALGLGWGRYLASARDVGPSAGAFGAAAALVWY
                     LPRRLRLPGGLALFSYLGGFLLLTHRVWDVEHLIAATLGLLLGAVLTGNAPRLALPRR
                     RGFHALRSTPRLVIAGLVVLTGCLNIVCAFSPRADARFDPL"
BASE COUNT          554 a         1313 c         1358 g          559 t
ORIGIN      
        1 cgtcgtgcag ctcgcgcgcc agttgcgccc gctcatcgcg cacggcgccc tcctccagct
       61 cggcgtagag cgcgatggcg cgcagcaggc tggccgtctg gttggccacg atgcgcaggt
      121 acgcctcggt gtcgtgcgcc agcggccggc gcagcggagt gaccaccagc cagccgtagc
      181 tggtgccgtc ggcgctgatc gggtagcgct gctcgttcgc gccgtcgtcc gcctggccgc
      241 ccagctcgac cgcgaccgtc gcctcgggcg ccaccacccg cgccgcccgg ctggccgcct
      301 cggagaccag cgtcgggtcg cggttgacga ccggcgtctg gctgagtgcc agcagcgccg
      361 aggtgcgcac ctgctgccgg cgggcctcgc tggaggcgcg cacgaactgg ctgccgaggt
      421 agaccagcag cagcaggtag gagacgagcc agaggaagtc ggcggtcgtg atagccgggt
      481 ggaagggcgc cgggtagaag acgaactgca cctcggccac cgccgccgtg atcagcgaca
      541 gcgccagcca ggcgttctcg ccctcgcgta ccgcggccgt caagcgcagc gccagccagc
      601 agtagaggag cgcgacgacc gactccagca cgatgcccag ctcggtctgc cccgccagga
      661 tcgggatggt gatcgggtcg cggcggaaca tcaagggcgc ccaggcgtcg cggccgagga
      721 gtcgcggcag ccagccacgc ccgtaccaga ggccgacgtc gaccgcggtg accagcgcca
      781 ccaccccggc cagccagagc tcggcgcgcc gcggcggcag gcggcccggc gtgcgccggg
      841 ccaggcccag cagcagcagc accaccacca cgaagcgggt gaagagccag ccgtagagcg
      901 ccgccgaggc cgagccgtgg aagggatgac ccttgaaggg gaggatcagc ccgaaccaga
      961 gcgagctgac cgccaccgcg ctgaaggcgg cggcgatcgc caggtcgagc gggcgacggt
     1021 cgaagacgta gcgcgccagc ccgacgaagg caaccatcgt cgcgaccagg gcggtgaagg
     1081 tgtcgatggt gaagctgagc gcgtcgtgcc ggaaggtgac gcgcagactg ggatcgacga
     1141 cgagcagcag cgtgctgacg ccgaggatga gccaccaggc gcgcaccaac tggaccgcgc
     1201 cgctcacgcg ctccccgcca agtctgccga tgcccagcgg tgccaaccgc gccgccacgt
     1261 tcctcgctgc cacgctcacc gccagccgtc cacgtccacc gcgcagtata cgaccttacg
     1321 tcgaccgagg cgccgcaaac ggcagcgatg tacccccttt ggcgtatccc catggtggga
     1381 gtcggccggg aggtagacac gtctccgggt ggcagaggcg gcgaggcgcc agccggggcg
     1441 aggagtcgag cgtcctcacg ccccggctgg tgtgtgatca cacgcgctcg tcgtccgagc
     1501 gttccgacga gtcctggcga caagtcgctg atcaggagaa ggcgttgccg atcaggatct
     1561 gcgtgtagcc gctgaggtcc ggcagcgagt tgtagacccg gatctgatcg cgcgagacgc
     1621 cgaagcggcc catattgtag gtcggcatcg tgcccgaacc gccccgcagc gtgatggtgc
     1681 cgaccggctt gaagtcgagc gtgacccaca gcacggtgcc gtcgggcaga ttgacgtcct
     1741 tgacctgaac ggagaggagg ctgaagccgc cgcagccgct gaatttcgac tcatcggcgg
     1801 tcgcctgacc gcgcggcgtc ttgccgttga gcgtggtgcc gctcaggctc tccttgaggg
     1861 tgccgccggt ctgacagccg gacgggctcg gcgcgggcga gttcgtgacg gtgagcgaga
     1921 acgtgcccgt cgccgtgttc cccgcggcgt cggtcactgt gacattgaac gtctgcgtgc
     1981 cgaggatggt cggatcaccg agcaggagac ccgagggccg cagcgtcagt ccggcgggaa
     2041 cctggcccga ccaggtgtac ggctgcgcgc cgccgctggc caggaacgag ttggtgtaga
     2101 agttgtttac gaagacgttg ctgcccgcgc cgatgtcgca gacgccgttg gcgcaactga
     2161 agaactggtc gttgatcgcc agcggggcgg cggtcccggt cccgccgccg gtcaacggca
     2221 ccgactgcgg gctgtccggc gcgttgtcgg tgaacgtgac gttctccgag cgcgtgccgg
     2281 tggtggttgg cgagaaggtg accgcgatgg tgcagctggc gccgacggcg agcgtcgtgc
     2341 cgatgcactg gtcatcgctg atggtgaagt cgagtccgtc ggctccggtg agcgcgacgt
     2401 tgttgaagaa gagcgaggcc gtcccggtgt tggtcgccgt gatcgtctgg ggcgcgctgg
     2461 tcgtcccggt atcctgcgcg gcaaaggtca gcgacgtggg tgagagcgag agcgccggcg
     2521 tccccgacgc ctggacaccc gctaccaacg agagcgctac cagagcggcc gcgaagacgc
     2581 cgaacagccg cgcgcgtcgt tgccggcgcc gcagcgccgc gtggacggcg gcgaataagg
     2641 catacgtccg atgctgcatg tcgttctccc ttcgtcgaga cggtgcagta gcggccgcta
     2701 cggctgctct acgcggcaaa gagccggtcg gatgttgccc gaacgggaca tagttggcgg
     2761 ctggcggaca ggttgggatc ggtgcgtgga gctgagaaga tccgcggcgt ccgacgcccc
     2821 aggagccggc gccggcaaaa tacccccttt agcgtatccc ctccggtgcg agccacggtc
     2881 aatacttcgc cggtccctgg cggctcgaaa gtgcggcgga gccggcgcga acgcggggca
     2941 cggctgaacg gataacgaca cggacacgga caccaagacg gcaaccaccc aaggagtctt
     3001 tctctcatga gctcacggct tgagcggctc gacgggcgcg tcgtgctcgg ttggctggcc
     3061 cgcgcgccct tcacgctggc gctgctggcg gtcatgctgg cgctggggat cgccaccggc
     3121 tcgatccggc accagttgcc gggcagcggc ctgctcgcgc gggtcggttt cggcctgccg
     3181 gcgctccagc acggccggta ctacacgctc gtcacctgca tccccttcgc cctctacccc
     3241 tggatgctgg ccacgatcac gctgctggtc gtgctcttga tgctccccta cgaggtcgtc
     3301 gccggcaccc gccgcgccgc gctcgtcttc ctcgcctgcc agctgggcgg ctacctgctg
     3361 gcggcgctgc tggtggcctg gccgctgagc gcgctcggcc tcggctgggg tcgctatctg
     3421 gcctccgcgc gcgatgtcgg cccctcggcg ggcgccttcg gcgccgcggc ggcgctggtc
     3481 tggtacctgc cgcgtcgcct gcggctcccc ggcggcctgg cactctttag ctacctcggc
     3541 ggcttcctgc tgctgacgca ccgtgtctgg gacgtcgagc atctcatcgc tgccacgctt
     3601 ggtctcctgc tcggggccgt gctcaccggc aacgcgccgc gcctagcgct gccccggcgg
     3661 cgcggcttcc acgcgctgcg ctccaccccg cgcctggtca tcgccgggct ggtggtgctg
     3721 accggctgcc tgaacatcgt ctgcgccttc agcccgcgcg ccgacgcccg cttcgacccc
     3781 ctcg
//
LOCUS       DASUBI010000119         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1291180, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000119 DASUBI010000000
VERSION     DASUBI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291180"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1915
                     /locus_tag="VFU72_02290"
     CDS             293..1915
                     /locus_tag="VFU72_02290"
                     /inference="COORDINATES: protein motif:HMM:NF006020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HEU5422344.1"
                     /translation="MSAVSYTAQTPLSFLERSARVWPEKVAVIYGSQRLSYAEFAAEA
                     VRVAGALRASGVEPGDRVAYLMPNLPEMLIAHFAVPLAGGVLVAINTRLTAQEVSYIL
                     RHSGAKIFVTDAALLPVAAAAADGIGTVTQLVVATDAEAGTVRPDLAARPGTVSYPEF
                     LAGQTADPLPWSVPDELAPIAIDYTSGTTGRPKGVIYTHRGAYLNSYAQIIHSRHDEN
                     SVLLWTLPMFHCNGWCAPWAVTAIGGTHVCLRDVRGDAIWQQLRQHGVTHLNAAPTVV
                     STILNASAAGPLPRPVLITTAGAPPSPTTIAEMERMGFTVVHVYGLTETYGPITFCQF
                     QPGWADLTVDQRAALQARQGVGMVTAGGVRVVDDKMADVPADGKTMGEIVMRGNTVMA
                     GYYLDPEATAEAFRGGWFHSGDLGVMYPDGYIELRDRAKDIVISGGENISTVEVEQAL
                     MSHPCVLEVAVVGVPDDKWGERPKAYVVLRAGTSATQQELIAHVRTKIAGYKAPREIA
                     ITMELPKTSTGKIQKFVLREKEWAGHGHRVKG"
BASE COUNT          335 a          681 c          686 g          317 t
ORIGIN      
        1 cagccgacac caccttgtcg ccgagagctt tgcatcggat gcgtgagcag cctgccatcc
       61 gctctcccct acgcccggat atcacggtgg tccgcaacgt ctctcccggc cgaccgcacc
      121 aggtcggcgc ctcagtcaga cgacacggta gacagccgct caggcgtccg tcggagcagg
      181 aggacccgac cggctgggac cttggtctct tccggaggta gccgtcgtgc cttggggtga
      241 gcaaaggccg cccagcgctc ggtcacgagc ggcttgcctt aggaggtcag acatgagcgc
      301 agtttcgtac acggcccaga caccgctatc gttcctggag cggtcagctc gcgtgtggcc
      361 ggaaaaggtc gccgtcatct acggtagtca gcgcctcagc tacgccgagt tcgcggccga
      421 ggcggtgcgg gtggccgggg cgctgcgagc cagtggcgtc gagcccggcg accgggtcgc
      481 ctacctgatg ccgaatctgc ccgagatgct catcgcccat ttcgcggtgc cgctggccgg
      541 cggcgtgctt gtcgcgatca atacccgtct cacggcccag gaggtgtcgt atatcctgcg
      601 ccactcgggg gcgaagatct ttgtgactga cgccgccttg ctcccggtgg ccgcggctgc
      661 cgcggacggc atcggcacgg tcacccagct ggtcgtagcc acagatgccg aggccggaac
      721 ggtccgcccg gatctggcgg cccgtcccgg cacggtaagc taccccgagt tcctcgccgg
      781 ccagacggcc gatccgctgc cctggtcggt cccggacgag ctcgctccca tcgcgatcga
      841 ctacacctcc ggcaccaccg ggcgcccgaa aggcgtgatc tatacccacc gtggcgccta
      901 cctgaattca tatgcccaga tcatccattc ccggcacgac gagaacagcg tgctcctgtg
      961 gacgctgccg atgttccact gcaacggctg gtgcgccccg tgggcagtga ccgccatcgg
     1021 cggcacgcac gtgtgcctgc gcgacgtgcg cggcgatgcg atctggcagc agctgcgcca
     1081 gcacggcgtc acgcacctga acgcggcgcc caccgtggtg agcactatcc tgaacgcgag
     1141 tgcggccggg ccgctgccgc ggccggttct catcaccacc gctggcgcgc cgcccagccc
     1201 caccacgatc gcggagatgg agcgcatggg gttcacggtg gtgcatgtct acgggctgac
     1261 cgagacctac ggcccgatca cattctgcca gttccagccg ggctgggctg acctgaccgt
     1321 cgaccagcgc gccgcgctgc aagcgcgtca gggcgtgggg atggtcacag ccgggggcgt
     1381 gcgcgtcgtg gacgacaaga tggccgacgt tccggccgat ggcaagacga tgggcgagat
     1441 cgtcatgcgc ggcaataccg tcatggcggg ctactacctg gatcctgagg ccaccgccga
     1501 ggcgttccgc ggcggctggt tccacagcgg cgatctcggg gtcatgtatc ccgacgggta
     1561 catcgagctg cgcgaccgcg ccaaggacat cgtgatctcc ggcggggaga acatctccac
     1621 cgtggaggtg gagcaggcgc tgatgagtca cccgtgcgtc ctggaggtgg cggtcgtcgg
     1681 ggtacccgac gacaagtggg gcgagcgccc gaaggcttac gtggtgctgc gcgccggcac
     1741 cagtgcgacc cagcaggagc tgatcgcgca tgtgcgaacc aagatcgccg gctacaaggc
     1801 accccgtgag attgccatca cgatggagct gccgaagacc tcgaccggaa agatccagaa
     1861 attcgtcctg cgtgaaaagg aatgggccgg tcacggccac cgcgtgaagg gttaaccagc
     1921 ccggaaagcg gtgcctgtcg ccgggagaag ctgacgcagg gagggcggca gatggcctgc
     1981 tccctccgtg tggctgggac gaacggctca ccggctatc
//
LOCUS       DASUBI010000120         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_105627, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000120 DASUBI010000000
VERSION     DASUBI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105627"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFU72_02295"
     CDS             complement(<1..1124)
                     /locus_tag="VFU72_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5422345.1"
                     /translation="MNIQRFTEKAQEAIVAAQREAESQRHAQLDVDQLLYALVTQSEG
                     IVPQVLQRAGIDPRVAVEDLRQVLEAAPKLQYSAQPSLGAGLRQVLQRAEEDAQAMGD
                     EYVSTEHLLIAALEVAPNAASVKALNRLGLTKDTALEALSQLRRNQRVTSQTPESTYA
                     ALEKYGRDLTELARKGKLDPVIGRDDEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAERIVRGDVPEGLKDKKIVQLDLGAMVAGAKFRGEFEERLKAALQEIAQSEGQIIV
                     FIDELHTLVGAGGAEGAVDAANMLKPALARGDLRCIGATTLDEYQKHVEKDAALQRRF
                     QPVFVGEPSVEDTISILRGLKEKYELHHGVRITDGAIVAAA"
     gene            complement(1183..1530)
                     /locus_tag="VFU72_02300"
     CDS             complement(1183..1530)
                     /locus_tag="VFU72_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5422346.1"
                     /translation="MRENEPLYVISVAARLLELHPQTLRKYEREGFVSPSRTAGNLRL
                     YSSEDLERLRQVKYLVEEVGVNLAGVQLAMDLTARLKRLRQQTAQLSRRNDGRLDPLV
                     DELEQVLHMLGAE"
     gene            complement(1741..>2189)
                     /locus_tag="VFU72_02305"
     CDS             complement(1741..>2189)
                     /locus_tag="VFU72_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422347.1"
                     /translation="GRQPAHRSVRRHEHLPRPVMGGSQSLGQRAPRHVLEGSAATNSS
                     GSSRAALSPAQSCVASGVTLAAAGNYQRATDQFQRALELDSSLDLARAPGFWSLGSAG
                     YLAAARAYARRDRLADASSLLAIVKLGCASRRDLEALLQEVMVPSR"
BASE COUNT          335 a          773 c          707 g          374 t
ORIGIN      
        1 gcggcggcga cgatcgcgcc gtcggtgatg cgcacgccgt ggtgcagctc gtacttctcc
       61 ttgagcccgc gcaggatcga gatggtgtcc tcgacgctgg gctcgccgac gaacaccggc
      121 tggaagcgcc gctgcagcgc cgcgtccttc tcgacgtgct tctggtattc gtcgagcgtg
      181 gtcgcgccga tgcagcgcag atcgccgcgc gccagcgccg gcttcaacat gttggcggcg
      241 tcgaccgccc cctcggcgcc gcccgccccg accagcgtgt gcagctcgtc gatgaagacg
      301 atgatctgcc cctcggactg cgcgatctcc tgcagcgccg ctttcagccg ctcctcgaac
      361 tcaccacgga acttggcccc ggcgaccatc gcgccgaggt cgagctgtac gatcttcttg
      421 tccttcagcc cctcgggcac gtcgccacgc acgatgcgct cggccaggcc ctcgacgatg
      481 gccgtcttgc cgacgcccgg ctccccgatc agcaccgggt tgttcttggt gcggcgcgag
      541 agcacctgga ccacgcgacg aatctcgtcg tcgcgcccga tcaccggatc gagcttgccc
      601 ttgcgtgcca gctcggtcag gtcgcgcccg tacttctcca gcgccgcgta ggtcgactcg
      661 ggcgtttggc tggtcacacg ctgattgcgc cgcagctggc tgagggcctc cagcgccgtg
      721 tccttagtca ggccgagccg gttcagtgcc ttgaccgaag ccgcgttggg cgcgacctcc
      781 agcgccgcga tcagaaggtg ctcggtgctg acgtactcgt cgcccatcgc ctgcgcgtcc
      841 tcttcggcgc gctgcaacac ttgccgcagt cccgctccga gggagggctg ggcgctgtac
      901 tgcagtttgg gcgcggcctc cagcacctgc cgcagatcct ccacggccac acgcgggtcg
      961 atccccgccc gctgcaagac ctgcggcacg atgccttcgc tctgcgtcac gagcgcatag
     1021 aggagctgat cgacgtcgag ctgcgcgtgc cgctgagatt ccgcctcacg ctgcgccgcg
     1081 acgatcgcct cctgcgcctt ctcggtaaat cgctgaatgt tcatccctgt cacctgcttc
     1141 ttgctgcccc ggctcgcgcg agccggtcat cgccttcgtc tgctattcgg cgccgagcat
     1201 gtgcagcact tgctccagct cgtcgaccag ggggtcgagc cggccatcat tgcgccgcga
     1261 gagctgcgcg gtctgctgcc tcaggcgctt caagcgcgcg gtcagatcca tcgccagttg
     1321 cacgcccgcg aggttgaccc cgacctcctc aaccagatac ttgacctggc gcaggcgttc
     1381 gaggtcttcc gaggagtaca ggcggagatt ccccgccgtg cgcgacggcg agacgaatcc
     1441 ttcgcgctcg tacttgcgta gcgtctgcgg gtgcagttcg agtagtctgg cagccacgct
     1501 gatgacgtag agcggctcgt tctcccgcat cgttcctccc gaggccggca tttacacgcc
     1561 tggagtatag cagtttatat gagtcatatc aagcttcccc aattcaagga cgcaggccaa
     1621 ttgcggctca gacctgcggc ggcctcatcg ctctgccgca caccccgtac cgccagccgc
     1681 acaggacaaa aaaagaggaa ggcgaaacgc cttcctcaaa ggtgggaggg aaagagatgg
     1741 tcagcgggaa gggaccatga cctcctggag cagcgcctcc agatcacggc gggacgcgca
     1801 cccgagcttg acgatagcca gcagggagct cgcatcggcc aggcgatcgc gccgagcgta
     1861 ggcgcgggcc gccgccagat agcctgccga ccccagggac caaaagccgg gcgcgcgcgc
     1921 cagatcgagc gaggagtcca actccagcgc gcgctggaac tggtcggtcg cgcgctggta
     1981 gttgccggcg gccgccaggg tgacgccgct ggccacacag gactgagccg gcgagagcgc
     2041 ggcgcgcgac gatccgctcg aattcgtcgc ggctgagcct tcgagcacat ggcgcggcgc
     2101 gcgctggcca agagactggc tgccgcccat taccgggcgc ggcaggtgct cgtgtcggcg
     2161 cacgctgcgg tgagcgggct gtcgaccgc
//
LOCUS       DASUBI010000121         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1359177, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000121 DASUBI010000000
VERSION     DASUBI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359177"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFU72_02310"
     CDS             complement(<1..237)
                     /locus_tag="VFU72_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5422348.1"
                     /translation="MNTGAALIGLVPLGVAALHVAPVLARTEAGRRLFPALARTGDPG
                     AVALSFDDGPDRQLDAFLALLREHGATATFVVTGE"
     gene            635..1873
                     /locus_tag="VFU72_02315"
     CDS             635..1873
                     /locus_tag="VFU72_02315"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422349.1"
                     /translation="MSTRTHAGIFATPSAIPEALRMLRETPAPDTGVLSVYLDTSVGR
                     LQGRAYLLAFRAGAKALRDEPQATPLPEGKAFEQAVARVDDYLLHTFVPRAPGVALFA
                     AASGDYFVVTPLPRRPSEDIAWAPLPRLSQLEEVLDDFERVAVALTDKQTLRLFTLYL
                     GELQEHAGIVSELPGQKAAGDWPNRVRTSPPRQDTSAPNRLPAGAWTGMIEGRQARRH
                     DEFVQRHSRAAAHALVDLLRREPFDRLFVAGPEEPAELLAKSLPRPLRARLAGIFPLP
                     VTASAAEVRQAALLAAEAVERQEELDTVDRLIQSAGSPYNALGLAATLEAASLGAIDR
                     LVITDTFAATGRECPQCGRLAAGGDRCPTCGAPMAGEVNLGERLVDQALAQGAQVEFV
                     SGQATERLARHGGVGAFLRY"
BASE COUNT          318 a          731 c          729 g          286 t
ORIGIN      
        1 ctcgccggtg acgacgaagg tggccgtcgc accgtgctcc cgcagcaggg cgaggaaggc
       61 gtccaactgg cggtcggggc cgtcgtcgaa gctgagcgcg acggcgcccg ggtcgccggt
      121 gcgcgccagc gccgggaaga ggcgccgccc ggcctcggtg cgcgccagga ccggcgccac
      181 gtgcagcgcc gccactccca acggcaccaa cccgatgagc gcggcgccgg tattcaaggc
      241 ggcggctccg tttcccatac ccgtcccacc cgtccccggg gatgctgtcg aacgaaggca
      301 cacggacatg tgaacggata gagaatattc tgataaacga cctgccgcgg gcgatcctgt
      361 tcttcccgta ggggatcgtg gacctggcca cgcggcccca cagctactat atgagcgagc
      421 tcaacaccag cgtcaacgcc catcgctggc gggtcgatgc ctcgtagcgg aacccggcgc
      481 cgcgccggcc ccccaggaac tgcctggggg cggcctctcc ctgagcacga ggtatatgga
      541 ccaccggccg gcgcgccgag tggtcccgat cctgcggcga cgccctcatc gggtcgatgg
      601 tctggaacat acgccagcga cgaagggaga ccccatgtcg acacgaacgc acgcgggcat
      661 cttcgcgacg ccgagcgcga ttcccgaggc gctgcgaatg ctccgcgaaa ccccggcgcc
      721 ggacacagga gtgctctccg tctacctcga tacctccgtc ggccgcttgc aggggcgggc
      781 ctatctgctc gcctttcggg cgggggctaa ggcgctgcga gacgagccgc aggccacgcc
      841 cctgccggag ggcaaggcat tcgaacaggc agtcgcccgg gtcgatgact atctgctgca
      901 cacgttcgtc ccgcgcgctc ccggtgtggc gctcttcgcc gcggccagcg gggactactt
      961 cgtggtcact ccgctgccgc ggcggccatc cgaggatatc gcctgggcgc cgctgccgcg
     1021 gctcagccag ctcgaggagg tgctcgatga cttcgagcgg gtcgccgtgg cgctcaccga
     1081 caagcagacg ctacggctgt tcaccctcta tctgggagag ctccaggagc acgccggcat
     1141 cgtgagcgag ctaccgggcc agaaggcggc cggcgactgg ccgaaccggg tgcgcacctc
     1201 cccgccacgg caagacacga gcgcgccgaa ccggcttccg gccggcgcct ggaccggcat
     1261 gattgagggg cggcaagcgc ggcgccacga cgaattcgtg cagcgccaca gccgcgccgc
     1321 cgcccacgcg ctggtcgatc tgctgcggcg cgagccattc gatcgcctgt tcgtggcggg
     1381 gccggaggag ccggccgagc tgctggcgaa gagcttgccc cgcccgctcc gcgctcgcct
     1441 cgccggcatc ttcccattgc cggtgacggc cagcgcggct gaggtgcggc aggccgccct
     1501 gctcgcggcc gaagccgtcg agcgtcagga ggagctggac acggtggacc ggctcatcca
     1561 gtcagcaggc tccccctaca acgcgctcgg gctcgctgcg acgctcgaag ccgccagtct
     1621 cggagccatc gaccggctgg tcatcacgga taccttcgcg gccaccggcc gcgagtgccc
     1681 acagtgcggc cggctggccg ccggcgggga tcgctgtcca acctgcggcg cgccgatggc
     1741 cggggaggtc aacctcggcg agcggttggt ggatcaggcg ctagcgcagg gcgcgcaggt
     1801 cgagttcgtc tcgggtcagg cgaccgagcg gctggcgcgg cacggcggtg tcggcgcctt
     1861 tctgcgctac tgagccaagc gagaaggaga tcggtgatgg cggaagagca gcgaatccag
     1921 gttcagggct tcgtgctgag cgacatcgag gagcgccgca tccggcacga cctggcgacg
     1981 atcgagcagc ggcttgcccc ctatggcgag ccgtatatca gcctggtgct gcgctggcat
     2041 cccgaccagc ggctggtgcg cgcc
//
LOCUS       DASUBI010000122         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_732772, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000122 DASUBI010000000
VERSION     DASUBI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732772"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1193
                     /locus_tag="VFU72_02320"
     CDS             480..1193
                     /locus_tag="VFU72_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF981 family protein"
                     /protein_id="HEU5422350.1"
                     /translation="MLHGLALALTVPWTQVPMYNTIMGLAAGAALVLLVWFGADLLRD
                     RPVEYEGWALAFGIVGLLQFVTGLHMTLTWPIAFIAPWDNIYFGETCLAFGTLLLAAA
                     FYLWRRSAVLAALERPALIAHIQRVIRPNSLFVFALGLALAWIGVFPLVFPTFGAPPE
                     EPISGLLSGYPRIESTLFALLYFALALGCLLFPWAIGSLNRTLLRIIALVWGVPGIVL
                     VLFVAMNFFTHGGLIYNTM"
     gene            complement(1204..2475)
                     /locus_tag="VFU72_02325"
     CDS             complement(1204..2475)
                     /locus_tag="VFU72_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422351.1"
                     /translation="MGRLSLVSRRGAIGAGSTIAGERHGGLFASLQLYRDYRLLWLST
                     VFTQIGQWMLTITLGWLILTLTNSAAWVGLVAFASGLPFILVSVPAGVLIDRVDQRRL
                     LVACQAAAIAIGLGLALLATLGLAQPWHLLLASFLNGAVLAINSATRQTLVPTFVARE
                     HLGNAIGLMSAGQNVTRILGPSLGGPAIALLGSAGALYLQSGFLLAALINTVMLPAVL
                     PRGGKALALRRNLTDGLAVIWHSPALLGLMILATIPSLLVFPYLQFLPVYARDILRIG
                     ASGLGLLFTASGIGAVTGALLVAGAQALPRKGQLILGGTLLYTLIIIGFAYARWVPLS
                     LLLIFGCGALGSFYMGLNNTLLHLHVDDEMRGRVMGVYTLTSGLSTLGALPMGFLGAR
                     IGVPSAIGIAAALTFLITAAAALRLRTLREL"
     gene            complement(2542..>3412)
                     /locus_tag="VFU72_02330"
     CDS             complement(2542..>3412)
                     /locus_tag="VFU72_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEU5422352.1"
                     /translation="ALWEEAGDRRMAGKAEAVAARLAEAPPAQVLYALLLDALGYTRN
                     RAPMQALAAALPYDHLDARLDGHASSERFARAAALLLGAGGFLPLTPRERELAGLEPE
                     PAARIEAAWLELGAPWRDLGLSPTSWTLARVRPAAHPLRRLLALATLLAHSGDGLLGA
                     MDRYVESTDAEGGLLVWLTGDNPYLGAAHAHEIIVNVVVPFALAYGEATGRDGLLERA
                     QTLWRELRAGAGNSEVRRTVEQICGPHRVPVRSARAEQGLLHLRRTGCAQLRCFECPI
                     AHLALHHEQPGAP"
BASE COUNT          520 a         1212 c         1212 g          468 t
ORIGIN      
        1 ggcgcgcatc gtgtcctcgg agccggcccc gccggccacg tagccgaagg cgccggggtc
       61 gaggatcgcc tccgcgcgcg tctcccaatc gtcgaaggcg accgggtgct ccagcggcac
      121 gcccgcaccg tagatcgcca actgccgctc caggcccggc cgcgccatcc gcccacctcg
      181 ctctcaacgc tcatgccatc acgggtatcg attggcacgg aggctagcgc ggcggccgcg
      241 ccgccgcaag agcaacgacc gccacctccg cgcgatcggc cccggtggac acgcgcggcg
      301 cggctggaac ccgacgtagc ccggcccctg gcgcatgatc gcgcgctccg gcgtcgcggc
      361 cgagctggat gcgtcctctc cgcaggaatt gcttcgaagg gacaaggggg caccggcaca
      421 attcctgcag ggtgcggacg ccgggggaga cctggatcaa atccggcagg agggcgtgga
      481 tgctgcacgg tttagcgctc gcgctgacag ttccgtggac tcaggttccc atgtacaaca
      541 ccatcatggg tctggccgcg ggcgcggccc tggtgctcct ggtctggttc ggcgccgacc
      601 tcctccgtga ccgccccgtc gagtacgagg gctgggcgct cgccttcggc atcgtcggtc
      661 tgctccagtt tgtgaccggc ctccacatga ccctcacctg gccgatcgcg ttcatcgcac
      721 cgtgggacaa tatctacttc ggtgagacct gtctggcctt cgggacgctc ctgctggcgg
      781 ccgcgttcta cctctggcgc cgctcggcag tgctcgcagc gctggagcgc ccggcgttga
      841 tcgcgcacat ccagcgggtg attcgcccca acagcctgtt cgtgttcgcg ctcggccttg
      901 cgctggcctg gatcggcgtc ttcccactcg tcttccccac cttcggggca ccccccgagg
      961 agccaatctc cggcctcctg tccggctatc cacgcatcga gtccaccctc ttcgcgttgc
     1021 tctacttcgc cctggcgctc ggttgcctgc ttttcccctg ggccattggc tcgctcaatc
     1081 gcacgttgct gcgcatcatc gccctggtgt ggggcgtgcc gggcatcgtg ctggtcctct
     1141 ttgtggcgat gaatttcttc acgcacggcg ggctgatcta caacacgatg tgacggcgag
     1201 cgatcagagc tcgcgcagcg tccgcaggcg aagggcggcc gcggccgtga tcaggaacgt
     1261 gagggcggcg gcgataccga tggcgctcgg cacgccgatg cgcgccccca gaaagcccat
     1321 cggcagcgcg ccgagcgtgc tcagaccgct ggtcagcgtg tagacaccca tcacccgccc
     1381 gcgcatctcg tcgtcgacgt gcaggtgtag cagggtgttg ttcagcccca tgtagaacga
     1441 gccgagcgcg ccgcagccga agatgagcaa caggctcagc ggcacccaac gcgcgtaggc
     1501 gaagccgatg atgatcaggg tgtagaggag cgtgccaccc agaatcaact gccccttgcg
     1561 tggcagcgcc tgcgcgccgg ccaccagcag ggcgccggtc accgcgccga tcccactggc
     1621 cgtgaagagc aggccgagcc cgctggctcc gatgcgcagg atgtcgcgcg cgtagaccgg
     1681 gaggaactgg aggtagggga agaccagcag cgacgggatg gtcgccagga tcatcagacc
     1741 caggagcgcc gggctgtgcc agatcaccgc caggccgtca gtcaggttgc gccgcagcgc
     1801 cagcgccttg ccgccgcgcg gcaggaccgc cggcagcatg acggtgttga tcagcgcggc
     1861 gaggaggaaa ccggactgca ggtagagcgc cccggcggag ccgagcaggg cgatggccgg
     1921 accgccgagc gacggcccga ggatgcgggt cacgttctgg ccggcggaca tgaggccgat
     1981 ggcgttgccc aggtgctcgc gcgcgacgaa ggtcgggacc agcgtctgcc gggtggcgct
     2041 gttgatcgcc agcaccgcgc cgttcaggaa cgaggcgagc agcaggtgcc acggttgcgc
     2101 gaggccgagc gtggccaaca gcgccagccc gagcccgatc gcgatcgcgg ccgcctggca
     2161 ggcgaccagc aggcgccgct ggtcgacccg atcgatcagc acgccggccg gcaccgagac
     2221 gaggatgaag gggagtccgg aagcgaaggc gaccagcccc acccaggcgg ccgaattggt
     2281 cagcgtcagg atcagccagc ccagggtgat cgtcagcatc cactggccga tctgggtgaa
     2341 gacggtgctc aaccagagga gccggtagtc gcggtagagc tggagcgagg cgaacagccc
     2401 gccgtgccgc tcaccggcga tcgtcgagcc cgcgccgatc gctccgcgcc gactgacaag
     2461 agagagccgc cccacgcact tcctccggtt ccaccagacg cagccggcgt agcttaccag
     2521 acaccccccg ccagcgcgcg gtcagggcgc gccgggttgc tcgtggtgca gcgccaggtg
     2581 ggcgatgggg cactcgaagc agcgcaactg agcgcagccg gtgcggcgta ggtgcaagag
     2641 cccctgctcg gcgcgggcag agcgcaccgg cacgcgatga gggccgcaga tctgctcgac
     2701 ggtgcggcgc acctcgctgt tgccggctcc ggcgcgtagc tcgcgccaga gcgtctgcgc
     2761 ccgctccagc agcccgtcgc gccccgtcgc ctcgccgtag gccagcgcga agggcacgac
     2821 cacgttgacg atgatctcgt gggcgtgcgc cgcgccgagg taggggttgt cgccggtcag
     2881 ccacaccagc agcccgccct cggcatcggt tgactcgaca taacgatcca tggcgccgag
     2941 gagaccgtcg ccggagtggg cgaggagggt ggccagggcc agcaggcggc gcagcgggtg
     3001 ggcggcggga cgcacgcgcg ccagcgtcca gctcgtcggg ctgagcccca ggtcgcgcca
     3061 gggcgcaccc agctccagcc aggccgcctc gatacgcgcg gcgggctccg gctccagccc
     3121 agccagctcg cgctcgcgcg gcgtgagcgg caggaagccg ccggccccga gcaggagcgc
     3181 ggcggcgcga gcgaaacgct ccgagctggc gtggccgtcg agccgggcgt ccaggtgatc
     3241 gtaggggagg gcggcggcca gcgcctgcat cggggcgcgg ttgcgcgtgt agccgagcgc
     3301 atcgagcagc agcgcgtaga gcacctgggc gggcggcgcc tcggccagcc gcgccgccac
     3361 cgcctcggcc ttgcccgcca tgcgccggtc gccggcctcc tcccagagcg cc
//
LOCUS       DASUBI010000123         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_255645, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000123 DASUBI010000000
VERSION     DASUBI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255645"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFU72_02335"
     CDS             complement(<1..277)
                     /locus_tag="VFU72_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5422353.1"
                     /translation="MAAREVITEGDARLRQKAIKIKRVDGELHKLAADMFETMHEHRG
                     VGLAAPQIGLLRRFIVIGLAEGMEEDGSPAVELALVNPEIVRASGRQI"
     gene            complement(551..3025)
                     /gene="priA"
                     /locus_tag="VFU72_02340"
     CDS             complement(551..3025)
                     /gene="priA"
                     /locus_tag="VFU72_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5422354.1"
                     /translation="MSAPDETVEAGCFVDVAVDTTVSDRERLLTYALPPFLRGRIGAH
                     QLVWVPLRRELKLAITVRIHQEPPGFGVRDVHAPVEPEFRFSPLQWELVEWLAERTLC
                     SLFEAAGPFLPPGVGQRSVEYLRLREGVDLAGLQLTRQQRELAELLGERGELSVEAAR
                     KALGRALTSVIPKLDELGVIERTARVRNRPLRPSTVRVVRLLPADGAELERAPAQRRA
                     LELVRRRAALAGGGALAWSELVRRGGSATALRALAGRGLIEIAEVPADGVATAPAGDG
                     EGRRVQLSAQQARVWGQLLQPLREERHQAFLLHGVTGSGKTEIYLRAAAWCLARGRQV
                     IVLVPEISLATQVVERFTERFPGQVAVLHSALPDAERYQTWQAVAAGRVPVVVGPRSA
                     LFAPLERPGLIVLDEEHEAAYKQDQPPRYHARAVALELARRSRAVLLLGSATPDVTSY
                     AAAERGELALLELPERAGPAVLGGSGREPARLELPAVEIVDMRLELQQGNTHIFSRAL
                     RQRLEQTLRSGEQAILFLNRRGTSTFVQCRACGHVESCPYCDIPLTYHADRAQLVCHR
                     CNERRPPPGRCPACGSDAIGYYGTGTQRVERETRLLFPEARVLRWDQDALRRGVNHAD
                     LVRRVLRHEADIVVGTQMVAKGLDFPLVSTIGVINADTLLHLPDFRAAERTFQLLTQV
                     AGRAGRRAGGGQVVVQSYTTDHYAIQTASRHDYAAFYREELAFRRRHGYPPFRRLVRL
                     LYRHRDELTCQSAAEEVAAELAYTAYRLRLTDIDLLGPTPAFTARVRGAYQWQILLRG
                     RDGPALAAAVPLSPGWQIDVDPMSLL"
BASE COUNT          479 a         1244 c         1248 g          470 t
ORIGIN      
        1 cgatctggcg cccgctggcg cgcacgatct cggggttgac cagggccagc tcgacggcgg
       61 gtgagccgtc ctcctccatg ccctcggcca gcccgatcac gatgaagcgc cgcagcagcc
      121 cgatctgggg agccgctagc ccgacgccgc ggtgctcgtg catcgtctcg aacatgtcgg
      181 ctgccagctt gtgcaactcg ccgtcgactc tcttgatctt gattgctttt tgtcgcagtc
      241 gggcatcacc ctcggtgatg acttcgcgtg ctgccatcgt cggttcgttt cctcggtatg
      301 ttgtcgggtc gaatcatgcg tttcgacgcg ccctcgcggc gccgccgcgc ccccggcgtg
      361 cctgcataag tatacggcac ggaccggtct ggcggcgggt tggcggcctt cgaggaggcg
      421 ccgcgccccc gagagagggc ggacacgggg gtccgcccct acaggaaacg ggccgtgcct
      481 accttgtcgg ggcagacccc tgtgtctgcc cactcaccct gccacatcgc tcgcccaaaa
      541 tgtgctgatc tcacaacaag ctcatcgggt cgacgtcgat ctgccagccg ggggagagcg
      601 gcacggcggc ggccagcgcc gggccgtcgc gcccgcgcag caggatctgc cactggtagg
      661 cgccccgcac acgggcggtg aaggcgggcg tcgggccgag caggtcgata tcagtcaggc
      721 gcaggcgata ggcggtgtag gccagctcgg cggccacctc ctcggccgcg ctctggcagg
      781 tgagctcgtc gcggtggcgg tagaggaggc gcaccaggcg acggaagggc ggatagccgt
      841 ggcggcggcg gaaggccagc tcctcgcgat agaaggcggc gtaatcgtgc cggctggcgg
      901 tctggatggc gtagtggtcg gtggtgtagc tctggaccac cacctgcccg ccgccggccc
      961 ggcgcccggc gcgtcccgcc acctgggtca aaagctgaaa ggtgcgctcg gcggcgcgaa
     1021 agtcgggcag atgcagcagc gtgtcggcgt tgatcacgcc gatggtcgag acgagcggga
     1081 agtcgagccc tttggccacc atctgggtgc cgaccacgat gtcggcctcg tggcgcagca
     1141 cgcggcgcac cagatcggcg tggttgaccc cccggcgcag cgcgtcctgg tcccagcgca
     1201 gcacgcgcgc ctcggggaag aggaggcgcg tctcacgctc gacgcgctgg gtgccggtgc
     1261 cgtagtagcc gatggcgtcg ctgccgcagg ccgggcagcg ccccggcggg gggcggcgct
     1321 cgttgcagcg gtggcagacg agctgggcgc ggtcggcgtg gtaggtgagc gggatatcgc
     1381 agtaggggca gctctcgacg tgcccgcagg cgcggcactg cacgaaggtc gaggtgccgc
     1441 ggcggttgag aaagaggatc gcctgctcgc ccgagcgcag cgtctgctcc agccgctgcc
     1501 gcagcgcgcg gctgaagatg tgggtgttgc cctgctgcag ctccagccgc atgtcgacga
     1561 tctcgaccgc cggcagctcc agccgggcgg gctcgcggcc cgaaccgcca aggaccgccg
     1621 gcccggcgcg ctcgggtagc tcgagcagcg ccagctcgcc gcgctcggcg gcggcgtagc
     1681 tggtcacgtc aggcgtggcg ctgcccagca gtagcacggc cctgctgcgc cgtgccagct
     1741 ccagcgccac cgcgcgggcg tggtagcgcg gcggctggtc ctgcttgtag gccgcctcgt
     1801 gctcctcatc gagcacgatc aggcccggcc gctccagggg cgcgaagagg gccgagcgcg
     1861 ggccgaccac caccgggacg cgcccggccg ccaccgcctg ccaggtctga tagcgctcgg
     1921 cgtcgggcag cgcgctgtgc agcacggcca cctggccggg gaaacgctcg gtgaagcgct
     1981 cgaccacctg ggtcgccagg ctgatctccg gcaccagcac gatgacctgc cgcccgcgcg
     2041 ccaggcacca ggcggcggcg cgcaggtaga tctcggtctt gccgctgccg gtgaccccgt
     2101 gcagcaggaa cgcctgatgc cgctcctcgc gcagcggctg gagcaactgg ccccagaccc
     2161 gcgcctgctg cgccgagagc tgcacgcgcc gcccctcgcc gtcgccggca ggcgccgtcg
     2221 cgactccgtc ggccggcacc tcggcgatct cgatcagacc gcgccccgcc agcgcccgca
     2281 gcgccgtcgc gctgccgccg cgccgcacca gctcgctcca ggcgagcgcg ccgccgcccg
     2341 ccagcgccgc gcgccgtcgc accagctcca gcgcccggcg ctgggccggc gcccgctcga
     2401 gctcggcgcc gtccgccggt agcagccgga cgactcgcac cgtgctcggc cgcagcggcc
     2461 gattgcgcac ccgcgccgtg cgctcgatca cccccagctc gtcgagcttg gggatgacgc
     2521 tggtcagcgc gcggcccagc gccttgcgtg ctgcctcgac gctcagctca ccacgctcgc
     2581 cgagcagctc ggccagctcg cgctgctgtc gagtcagctg cagcccggcg aggtcgacgc
     2641 cctcgcgcag ccgcagatac tcgacgctgc gctgcccgac ccccggcggc aagaaggggc
     2701 cggcggcctc gaagagcgag cagagcgtgc gctcggccag ccactcgacc agctcccatt
     2761 gcagcggcga gaagcggaac tccggctcga ccggggcgtg aacgtcgcgc acgccgaagc
     2821 cgggcggctc ctggtggatg cgcacggtga tggcgagctt cagctcccgg cgcagcggca
     2881 cccagacgag ctggtgcgcg ccgatgcgcc cgcgcaggaa aggcggcagg gcgtaggtca
     2941 gcagccgctc gcggtcggat accgtcgtat cgacggccac atcgacaaag cagccggcct
     3001 ccaccgtctc gtctggcgcg ctcatggcgc aagtgtagca gggggtgggg gtgaacgttc
     3061 gggtgggagg atggcctacg atcaggttgc gtggcgatcc cggcccgcgc ggggctgaag
     3121 ccaccgcgct agcagacgaa gcctgctgca gcaggctgaa gctcaacccc cagcctgctg
     3181 cagcaggttt tcggaaagat cgagcgcggt ggttctaacc ccgcgagtcg ggcgagacgc
     3241 gcgcctggga tgcgcgcgaa ccttccttca acccctcgcg caacgaggca cgcctgcccc
     3301 ggatcacccc tccatccccg cgtggatggt ctccgcgtcg gcgaagaggg cgggcacgcc
     3361 gccgctgtgc aggaagatca ccgtctcgct cgggcgcacg gcgccgtcgc ggatgtcggc
     3421 cagcatgccg gcgaaggctt t
//
LOCUS       DASUBI010000124         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_418394, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000124 DASUBI010000000
VERSION     DASUBI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418394"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFU72_02345"
     CDS             complement(<1..671)
                     /locus_tag="VFU72_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5422355.1"
                     /translation="MRCSEARLHLSALLDQELAPKSAWAVRAHLDVCPRCRAIYREYA
                     ANQRQLDDLLRSTPSLPVAGGVLAVIRQAPRPTGPSRLARGGQRLLAGAALLALVAVV
                     TAGALAIRGAGQHRTAGLGATAAAPSSAVGASAKQPAASTLEATAPPVAAAISGFGAA
                     ASPAAHTAGATPAPVDPRLTALQATAGFTLYAPTWLPDGVTLAGAAPPKQLPGFVSLD
                     LPYRDA"
     gene            complement(672..1253)
                     /locus_tag="VFU72_02350"
     CDS             complement(672..1253)
                     /locus_tag="VFU72_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5422356.1"
                     /translation="MAGTNARAGGWGRRVDIAGQVRGADLVADFEDIVRTYEAPISRY
                     LYRMVGERELARDLAQETFLSAYRALPEAEITNLSGWLYRIATNHALAHFRRRKLIRW
                     VPLVKLLGTGSDPRSADHGEWVATSTAVEAALAKLDPRERACLLLKAAGFSSQEIGQQ
                     LGCSEGAARTRLSRARESFRRIYHQRDPGRLED"
     gene            complement(1324..2796)
                     /locus_tag="VFU72_02355"
     CDS             complement(1324..2796)
                     /locus_tag="VFU72_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5422357.1"
                     /translation="MARSASRSQAIDGDVLGPVTVRQIAIFVTVSLALLMSSIDSTIV
                     AVGLPNMMNGLHTNLVWIGWVLTGYSLTQTMILPMAGKFSDDFGRKRLFLACVVLFTT
                     SSLLCSIAPNVYILILCRVLQAVGGGAFMPSAAGIVSDVFGPQRRSTAIGLFTSVFPI
                     GGIVGPNVGGWVIDHFGWRAIFAVNVPIGVVLFLVGIVLLPAGGRSGTSRGIDLIGAL
                     SFGFGIAAFMYGMTVWGNNVTFDFQVALWMAAGLAALIYFLRHEARAVNPMIEIRLLK
                     ERAFFAANLYNFLYGSMVFGFFSFLPLYATMAYGMSATQAGFILTPRAIAMISLSAIS
                     SFLLIRFGYRLPMIIGVIFVSASLFLVSQGWHNPVIFGHQLSNLGLLSILIAIGGLGV
                     GIGGPAANNAAIELMPEAVARITGLRGMFRNTGGVLGTASIVLYMAHQQNQALGMEKL
                     FLILSVMVLLVIPPVFLIPDGAASRRKAAAAAIQPLVSGE"
     gene            complement(3207..5642)
                     /gene="lon"
                     /locus_tag="VFU72_02360"
     CDS             complement(3207..5642)
                     /gene="lon"
                     /locus_tag="VFU72_02360"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5422358.1"
                     /translation="MMSRYDMYSVRYPLLPLKNVVIFPRNVVTLLVGRTRSIQAVEEA
                     MSRDRRIVVVAHRVISSEDPQPDDLYQTGTLAEIASIEQQPNGNIQVALEGLCRVRLV
                     QFDTPRPFYTVRAEQAVERVPHTVEAQALVHHIHELAQRFQEVKSAFNGEVMDMVRAT
                     SDPSHLSDLLTTQLVRDSEQRQQFLENLDPLSRLELLTVHLTSDIDLAALEQRIKDRV
                     REQIDKNQREYYLREQLKVIHDELSGEGGNEMEAIRARIRERGLPEEVEEKLLKEVTR
                     LERMPTISAEGTVVRNYIDTVLGLPWNESSEDNLDLDHAEGILEADHYGLDQVKERIL
                     EFLAVRKLTGGRGVGASILCLAGPPGVGKTSLGKSIATAMNRQFVRVSLGGVRDEAEI
                     RGHRRTYVGAFPGRVVGAMKTAGTSNPVIVLDEIDKMTSDYRGDPASAMLEVLDPEQN
                     HAFNDHFLDMSYDLSKVLFIATANYLANVPRPLRDRMEIIEIGGYTEQEKMEIGKRHL
                     LPKQLAAHGLENGQIEIPEKIWMLLIRRYTREAGVRGMERQLASLCRKVAREVVKGRS
                     KKVRLNEARLEEYLGPQRFGFDHQVGESQVGVAIGLGTTDVGGELIPVEVAVMPGRGG
                     LTITGRAGDVMQESARAALSYARSRAEALKLAKDFQEKQDLHIHLPEGATPKDGPSAG
                     ITMATALISALTRRPVRADVAMTGEITLRGRVLPIGGLKDKTLAAHRAGIRRLIAPLD
                     NKQDIAKLPPKVASEMTFHWVENMDQVIAEALDFSEQVQEIDPSLREQLTTEQQPVAD
                     LSELPVEEAATDL"
     gene            6280..7062
                     /locus_tag="VFU72_02365"
     CDS             6280..7062
                     /locus_tag="VFU72_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEU5422359.1"
                     /translation="MELLQGKKGLVVGVSNDHSIGWGIAKALHAAGAELAFSYAGEPF
                     ERRVRPLAESLNAPLIAPCDVQSDAEITGLMDQVRAVFGSLDILVHAVAFAKREELSG
                     PYLNTSREGFRQAMDVSVYSLTALVKAAEELLNPGASIVTLTYHGSQQVAPHYNVMGL
                     AKAALEASVRYLAHDLGPRGVRVNAISAGPIRTLAASGISGFRALHKSFAEVAPLRRN
                     VTTEDVGHTATWLCSDWAAGVTGETIYVDAGFRNVMTQFPQE"
BASE COUNT         1188 a         2427 c         2528 g         1163 t
ORIGIN      
        1 gcatcgcggt aggggaggtc gaggctgacg aagccgggga gctgcttggg cggcgcggcc
       61 ccagccaacg tgaccccgtc cggaagccag gtgggcgcgt agagcgtgaa gccggccgtg
      121 gcctgcagcg ccgtcagccg cgggtcgacg ggcgccgggg tagcgccagc ggtgtgcgcc
      181 gccggggagg ccgccgcgcc gaagccggag atagctgccg ccacgggcgg cgcggtcgcc
      241 tccaacgtgc tcgcggccgg ctgcttggcg ctggcgccga cggcgctgct gggcgcggcc
      301 gccgtagccc ccagcccggc ggtccggtgc tgcccggcgc cacggatcgc cagcgcgccg
      361 gccgtcacca cggccaccaa cgcgagcagc gccgcgccgg ccagcaggcg ctgaccaccg
      421 cgcgccagcc gcgacgggcc ggtgggtcgg ggcgcctgcc ggatcaccgc gaggacgccg
      481 cccgccaccg gcagggaggg cgtgctccgc agcaaatcgt cgagctggcg ctggttggcg
      541 gcgtactccc gatagatcgc ccggcagcgt gggcagacgt ccaggtgggc gcgcactgcc
      601 caggccgatt tcggcgccag ttcctggtcg agcagcgcgg agagatggag gcgggcttcg
      661 ctgcagcgca tctagtcctc cagccttccc gggtcgcgtt gatggtagat gcgccggaac
      721 gactcgcggg cacgcgagag gcgcgtgcgg gccgcgccct cggagcagcc gagctgctgg
      781 ccgatctcct ggctggagaa gccggccgcc ttcagcagca ggcaggcgcg ctcgcgcggg
      841 tccagtttgg ccagcgccgc ttcgaccgcg gtgctggtgg cgacccactc gccgtggtcg
      901 gcgctgcgcg ggtcgctgcc ggtgcccagc agcttgacga gcggcaccca gcggatcagc
      961 ttcctgcggc ggaagtgggc cagcgcgtgg ttggtggcga tgcggtagag ccagcccgag
     1021 aggttggtga tctccgcctc cggcaaggcg cggtaggccg agaggaaggt ctcctgtgcc
     1081 aggtcgcgcg ccagctcgcg ctcgccaacc atgcggtaga ggtagcggga gatgggcgcc
     1141 tcgtaggtgc gcacgatatc ttcgaaatct gcgaccagat ccgctccgcg aacctgcccc
     1201 gcgatatcca cccgtctccc ccaaccgccc gcgcgcgcat tcgtcccggc catcggccca
     1261 tccttctgcc acgttcgatg cgcgggtgct tgaaatgtta cagggtaccg gccgggggcg
     1321 gcgctactcg ccggagacta ggggctggat agcggcggcg gccgccttac gccggctggc
     1381 cgcgccgtcc gggatcagga agaccggtgg gatgaccagc agcaccatga ccgagaggat
     1441 caggaagagc ttctccatgc cgagcgcctg gttctgctgg tgggccatgt agagcacgat
     1501 cgaggcggtg ccgagcacgc cgccggtgtt gcggaacatg ccgcgcagcc cggtgatgcg
     1561 cgccaccgcc tcgggcatca gctcgatggc ggcattgttg gccgccggac cgccgatgcc
     1621 gacgcccagc ccgccgatcg cgatcaggat cgagagcagg cccagattgc tgagctggtg
     1681 gccgaagatg accgggttgt gccagccctg gctgaccagg aagaggctgg cgctgacgaa
     1741 gatgacgccg atgatcatcg gcaggcgata gccgaagcgg atcaagagga aggagctgat
     1801 ggccgacagg ctgatcatgg cgatggcccg cggcgtcagg ataaagcccg cctgggtggc
     1861 cgacatgccg taggccatgg tggcgtagag cggcaggaag ctgaagaagc cgaagaccat
     1921 cgaaccgtag aggaagttgt agagattggc ggcgaagaag gcgcgctcct tcagcaggcg
     1981 aatctcgatc atcggattga cggcgcgcgc ctcgtggcgc aggaagtaga tgagcgcggc
     2041 cagtcccgcc gccatccaga gcgccacctg gaagtcgaag gtcacgttgt tgccccagac
     2101 ggtcataccg tacatgaagg cggcgatgcc gaagccgaag ctgagcgcgc cgatcaggtc
     2161 gataccgcgg ctcgtgccgg agcggccgcc cgccggcagc agcacgatgc cgaccaggaa
     2221 gaggaccacg ccaatcggca cgttgaccgc gaagatggcg cgccagccga agtggtcgat
     2281 cacccagccg ccgacgttcg ggccgacgat gccgccgatc gggaagacgc tggtgaagag
     2341 gccgatggcg gtgctgcgcc gctgcggccc gaagacatcg ctgacgatgc cggccgccga
     2401 gggcatgaag gcgccgccac cgaccgcctg cagcacccgg cagaggatca ggatgtagac
     2461 attgggcgcg atcgagcaga ggagcgacga ggtcgtaaag aggacgacgc aggccaggaa
     2521 gaggcgtttg cggccgaagt catcgctaaa cttgccggcc atcggcagga tcatcgtctg
     2581 cgtcaggctg tagccggtca gcacccagcc gatccagacg aggttcgtat gcaggccgtt
     2641 catcatgtta gggaggccga ccgccacaat ggtcgagtcg atgctcgaca tcagcagcgc
     2701 cagcgagacg gtgacgaaga tggcgatctg gcggacggtt accgggccga ggacatcgcc
     2761 gtcgatggcc tgcgagcgag aagcggagcg tgccatgctg tcccctcggt cgtggatctc
     2821 acgcgcccct ctccccgatt ccggacgcgt cgcgtgaata gccggccaac tgtacacccg
     2881 gtaccttgac catgcaacta tttgacgcga tgcaagggaa gagtatatcg caaggcgatg
     2941 gaacgcggcg agcggtgcgg ggaagggaca gtggcaaacg acgaggatgg ggcgtagggc
     3001 acgacggatc cttcgacccc gctcaggatg acagggttag agtcgggcga acgacgacga
     3061 ctcgtgtcgt ccgataggcc acatacctcc ccttgtcatc ctgagcggag tcgaaggatc
     3121 cgtgctgaag gggcacgcaa cgaagagggc gggtttgaaa cccgccccta cgaaatcttt
     3181 cgtgagctgt gcgtgcgggc ccgcgcttac aaatcggtcg cggcctcctc aacaggaagc
     3241 tcgctcaaat cggccaccgg ctgctgctcg gtggtgagct gctcgcgcag cgaggggtcg
     3301 atctcctgca cctgctcgct gaagtcgagc gcctcggcga tcacctggtc catattctcg
     3361 acccagtgga aggtcatctc cgacgccacc ttcggcggca gcttggcgat gtcctgctta
     3421 ttgtccagtg gggcgatcag ccggcgaatg ccggcgcgat gcgcggcgag cgtcttgtcc
     3481 ttgagcccgc cgatcggcag cacccgcccg cgcaaggtga tctcgccggt catggccacg
     3541 tcggcgcgca ccgggcggcg ggtcagcgcc gagatcagcg ccgtcgccat ggtgatgccg
     3601 gccgacggcc catctttggg cgtggcgccc tcgggcaggt ggatgtgcag atcctgcttc
     3661 tcctggaagt ccttggccag cttgagcgcc tcggcgcgcg agcgtgcgta gctgagcgcg
     3721 gcgcgagccg actcctgcat cacgtcgccc gcccggccgg tgatcgtcag cccgccgcgc
     3781 cccggcatga ccgcgacttc gaccgggatc aactcgccgc cgacatcggt ggtgcccagc
     3841 ccgatggcca cgccgacctg gctctcgccg acctggtgat cgaagccgaa gcgctgcggg
     3901 cccagatact cctccagccg cgcctcgttg agccgcacct tcttgctgcg gcccttgacg
     3961 acctcgcgcg ccaccttgcg gcagaggctg gccaactgtc gctccatgcc gcgcacgccc
     4021 gcctcgcggg tgtagcggcg gatcagcagc atccagatct tctcggggat ctcgatctgg
     4081 ccgttctcca gcccgtgcgc cgcgagctgc ttcggcagca ggtgacgctt accgatctcc
     4141 atcttctcct gctcggtgta gccgccgatc tcgatgatct ccatgcggtc gcgcagcggg
     4201 cgcggtacgt tggcgagata attggcggtg gcgatgaaga ggaccttcga caggtcgtag
     4261 gacatgtcga ggaagtgatc gttgaaggcg tggttctgct cgggatcgag cacctccagc
     4321 atggccgagg ccggatcgcc gcggtagtcg gaggtcatct tgtcgatctc gtcgagcacg
     4381 atcaccgggt tgctggtgcc ggcggtcttc atcgcgccca cgacacggcc agggaaggcg
     4441 ccgacgtagg tgcgccggtg gccgcggatc tcggcctcgt cgcgcacgcc gcccaggctg
     4501 acgcgcacga actggcggtt catcgcggtc gcgatcgact tgcccaggct ggtcttgccg
     4561 acgcctggcg gtccggccag acagaggatc gaggcgccga cgccccgccc gccggtcagc
     4621 ttgcgcaccg ccagaaactc gaggatacgc tccttgacct ggtcgagccc gtagtggtca
     4681 gcctccagga tgccttccgc gtggtcgaga tcgagattgt cctcgctgga ctcgttccag
     4741 gggagcccga ggacggtgtc gatatagttg cgtacgacgg tgccctcggc cgagatcgtc
     4801 ggcatgcgct ccaggcgggt gacctccttg agcagcttct cctcgacctc ctcgggcaga
     4861 ccgcgctcgc ggatacgcgc ccggatcgct tccatctcgt tgccgccctc gcccgagagc
     4921 tcgtcgtgga tcaccttgag ctgctcgcgc aggtagtact cgcgctggtt cttgtcgatc
     4981 tgctcgcgca cgcggtcctt gatgcgctgc tcgagcgcgg ccaggtcgat atcggaggtc
     5041 agatgcaccg tcagcagctc caggcggctg agcggatcga ggttctccag gaactgctgc
     5101 cgctgctcgg agtcgcgcac gagctgcgtg gtcagcaggt cggagaggtg cgagggatcg
     5161 ctggtggcgc gcaccatgtc catcacctca ccgttgaacg cgctcttgac ttcctgaaaa
     5221 cgctgcgcca actcgtggat gtggtgcacc agggcctgcg cctcgaccgt gtgcggcacc
     5281 cgctccacgg cctgctcggc ccgcacggtg tagaaggggc gcggcgtgtc gaattggact
     5341 agacggacgc ggcagagtcc ttcgagggcc acctggatat tgccgttggg ctgctgctcg
     5401 atcgaggcga tctcggcgag cgtgccggtc tgatagaggt cgtccggctg cgggtcctcg
     5461 gagctgatca cccggtgcgc gacgacgacg atgcgccggt cacggctcat tgcctcttcg
     5521 accgcttgaa tcgagcgggt ccggccgacg agcaacgtca cgacattccg cgggaagatc
     5581 acgacattct tgagcggcag cagaggataa cgaaccgaat acatgtcgta acggctcatc
     5641 atgagctatt tccttcttcc tgtgccattc ggcaatgcac accaggcacc agctccctca
     5701 ccagttcctg gctcgctcgc cgcccttgcg accagcggag cgccgtgacc caagcgcccc
     5761 gcctgtgctc gccaacccgc acatacgtga gcggtagatc gcggactggc ttctggtcta
     5821 tagggcggca ctgcccccca agtttacccg accccctccg aactgaaaag ggcgaccgag
     5881 gtcctaattg cccgcctttc gccgtcgtta atgcgaacgt tcgcgcggat tttctcgtcc
     5941 ggattcgtga cgaagcgcac aatctccccc gcagaattgc gcgaaaacgc aaccgtgcgc
     6001 gtcgcgcggc acgcgatagt gtcgcgatgg acccgtctag gaagctgaac gtgccagtcg
     6061 tcccccggtt gcgtccccgc cactgatgtc cgtaccatac gaacggacgg ggaccgccgt
     6121 ggtcgcgctc gctgtgccac aatgtcagcc gaggcgacgg cgcgagaggc tgccgggcta
     6181 gcccggctac cagctgaccg gccgccgctt cgccaggccg ctgctgcccg cgtatggtgg
     6241 cgcttgtcgc gccgcgggaa aagggagata gggagagcga tggagctgct gcaggggaag
     6301 aaggggctgg ttgtcggggt ctccaacgac cactcgatcg gctggggcat tgccaaggcg
     6361 ctgcacgccg ccggcgccga gctcgccttc agctatgccg gcgagccgtt cgagcgccgg
     6421 gtgcggccgc tggccgagag cctgaacgca ccgctgatcg ccccctgcga cgtgcagagc
     6481 gacgcggaga tcaccggctt gatggaccag gtgcgcgcgg tcttcggctc gctcgacatc
     6541 ctggtgcacg cggtggcctt cgccaaacgt gaagaactca gcggccccta cctcaacacc
     6601 tcgcgcgagg gcttccggca ggcgatggat gtcagcgtct actcgctgac ggccctggtc
     6661 aaggccgccg aggagctgct caaccccggc gcgtcgatcg tgaccctgac ctaccacggc
     6721 tcacagcagg tggcgcccca ctacaacgtg atggggctgg ccaaggccgc gttggaggcc
     6781 agcgtgcgct acctggcgca cgacctcggc ccgcgcggcg tccgggtcaa cgccatcagc
     6841 gccgggccga tccgcaccct ggcggccagc ggcatcagtg gcttccgcgc cctgcacaag
     6901 agcttcgccg aggtggcgcc gctgcgccgc aacgtcacca ccgaggatgt cggccacacc
     6961 gctacctggc tctgcagcga ctgggccgcc ggggtcaccg gcgagacgat ctacgtcgac
     7021 gccggcttcc gcaacgtgat gacccagttc ccccaggaat agcgccccgg gacacgagga
     7081 gggacgcgct cgccgcgcgt ccctcgtgcc aggcgcttga ggcgcggtgt ctaggagacg
     7141 gatccggaga gcgtgccggc ctcgcgaatc cactcttcga cctggctctg cgtcggtgcg
     7201 tggtgggtga tcttcttctc gtcgttggcc tgcttcagcg ccgcgtagag attctccggc
     7261 ttgcggtgct gcagctcctt gatcgagtcg acgccgcact cctcga
//
LOCUS       DASUBI010000125         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1141433, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000125 DASUBI010000000
VERSION     DASUBI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141433"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..605)
                     /locus_tag="VFU72_02370"
     CDS             complement(42..605)
                     /locus_tag="VFU72_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5422360.1"
                     /translation="MAHESSSYSIGDQEGRRAVDAYLDIVSLRAVRDYTDQPLGEQEL
                     ERILQAGRVAGSAGNRQPWTFVTITDRATLDKLAEIVYEPDSLHGAAAAVAVVVRTAN
                     QGFDAGRAAENMMLAAWAGGIGSSPNGLREREPAHDILSVPTDHYIANIITFGYPKKP
                     RQVDNKTPEGLLARVKRKPLSEIVRRI"
     gene            683..1627
                     /locus_tag="VFU72_02375"
     CDS             683..1627
                     /locus_tag="VFU72_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5422361.1"
                     /translation="MKPVQLLQLALLGLLWGSSYLFIKVAVKGIDPATLVEGRLVIGT
                     AVLFCLLRLRRRRLPRDPRLWFHLLVMAVVGVVLPQLLIAWSEQHIASSLAAILNATT
                     PFFTLCFAAGLFQTERFSAEKLGGLLIGFAGIAVLAGAGLADLAASSTRGELAMLLSS
                     VGYGAGFAYARRYLRGDAAVLAGGEMLLAALVAVPVVLATGHPGAMDLTLVRLVAWLA
                     LGAFSSGFAYILYFGLIESAGATRASFGTYLIPPVGVFWGWLLLGEGVGTRTIAGVVL
                     ILGGVALATWTRGNRAAPALAPTSAVQGEETVESARFD"
     gene            1656..2339
                     /locus_tag="VFU72_02380"
     CDS             1656..2339
                     /locus_tag="VFU72_02380"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEU5422362.1"
                     /translation="MQRDYILRLIEELGTFLKSVLEMRRRGQYQQAERLIDDQLMRMV
                     GFGMNVADQFPAATLVGMVRLGNVEERDQRIVAERLVVLGSLLQEGAEVDGDLGNERR
                     RDERRVKALHVFLIALTEEPTTPRRALASVDRLLEQLAEFDLPLDAKERLWQHFQQEG
                     QFARAEDWLYELLDDDRAPDDVLARGLAFYQRLSALSDDVLEAGNLPRDEVLAGLTQL
                     RARAGGQGE"
BASE COUNT          289 a          826 c          889 g          401 t
ORIGIN      
        1 gtgccatcct gcgccgggcg tgcggggaag ggtgagcccg cctagatacg ccgcacgatc
       61 tcgctgagcg gcttgcgctt gacgcgcgcc agcagcccct ccggcgtctt gttgtccacc
      121 tgccggggct tcttggggta accgaaggtg atgatgttgg cgatgtagtg gtcggtaggg
      181 acggacagga tgtcgtgcgc cggctcgcgc tcgcgcaggc cgttcgggct ggagccgatg
      241 ccgccggccc aggcggccag catcatgttc tcggccgcgc gcccggcgtc gaacccctga
      301 ttggccgtgc gcaccaccac cgccacggcg gcggccgcgc cgtgcaggct gtccggttcg
      361 tagacgatct cggccagctt gtcgagcgtg gcgcggtcgg tgatggtgac gaaggtccag
      421 ggctggcggt tgccggcgct gccggcgacg cgcccggcct gcaggatgcg ctccagctcc
      481 tgttcgccga gtggctggtc ggtgtagtcg cgcaccgcgc gcaggctgac gatgtcgaga
      541 taggcgtcca cggcccgccg tccttcctgg tcgccgatcg agtaagatga gctttcgtgc
      601 gccatgcgtg tgcgccaggc gcgtgcgcta cgccgcgcgg cacgccctgc gtctcgcgcg
      661 gatcgtgccc ggggagtctg ggatgaagcc ggtccagttg ctccagctcg cgctgctcgg
      721 cctcctctgg ggctcatcct acctctttat caaggtggcg gtgaagggga tcgatccggc
      781 cacgctggtc gagggccggc tggtgatcgg cacggcggtc ctcttttgcc tgctgcgcct
      841 gcgccgccgg cggctgccgc gcgacccgcg cctgtggttc cacctgctgg tgatggcggt
      901 ggtcggggtg gtgctacccc agctcctgat cgcctggagc gagcagcaca tcgcctccag
      961 cctggccgcc atcctcaacg cgaccacccc cttcttcacc ctctgcttcg cggccgggct
     1021 ctttcagacc gagcgcttca gcgccgagaa gctgggtggg ctgctgatcg gcttcgccgg
     1081 catcgcggtg ctggccgggg cggggctggc cgatctggcg gcgtcgtcga cccgcggcga
     1141 gttggccatg ctcctctcca gcgtcggcta cggcgccggc ttcgcctacg cccggcgcta
     1201 cctgcgcggc gacgccgccg tgctggccgg cggcgagatg ctgctggccg ccctggtggc
     1261 cgtgccggtc gtgctggcca ccggccaccc cggcgccatg gatctgacgc tcgtgcgcct
     1321 cgtcgcctgg ctcgcgctcg gcgccttcag ctccggcttc gcctacatcc tctacttcgg
     1381 cctgatcgag tcggccggcg ccacgcgcgc ctccttcggc acctacctca tcccgccggt
     1441 gggcgtcttc tggggctggc tgctgctggg tgaaggggtc ggcacgcgca ccatcgcggg
     1501 cgtggtgctg atcctgggcg gggtggcgct ggccacctgg acgcgcggca accgcgccgc
     1561 cccggccctc gcccccacct ccgccgtgca gggggaagag acggtcgagt cggcgcgctt
     1621 cgactgaggc gttcgcagcg gatggagatg acagcgtgca acgcgactac atcctgcggc
     1681 tgatcgagga gctgggcacc ttcctcaagt ccgtgctgga gatgcgccgc cgcgggcagt
     1741 atcagcaggc cgagcggctg atcgacgacc agctgatgcg catggtcggc ttcggcatga
     1801 acgtggcgga ccagttcccg gccgcgacgc tggtgggcat ggtgcggctg ggcaacgtcg
     1861 aagagcgcga tcagcgcatc gtggccgagc ggctggtggt gctggggagt ctcctgcagg
     1921 agggggcgga ggtcgacggc gacctgggca acgagcgccg gcgcgacgag cggcgcgtca
     1981 aggcgctgca cgtcttcctg atcgcgctga ccgaggagcc gaccacgccg cgccgggcgc
     2041 tggcgagcgt cgaccgcctg ctggagcagc tggccgagtt tgacctgccg ctcgacgcca
     2101 aggagcggct ctggcagcac ttccagcagg agggacagtt cgcccgggcc gaggactggc
     2161 tgtatgagct gctggacgac gaccgcgccc ccgacgatgt cctcgcccgc ggcctcgcct
     2221 tctaccagcg cctcagcgcg ctgagcgacg acgtgctgga ggccggcaac ctgccgcgcg
     2281 acgaggtgct ggccgggctg acgcagctgc gggcgcgagc ggggggacag ggggagtgag
     2341 aggtattctc gatggccgtg cgatcgggca agacggatcc ttcgactgcg ctcagagcct
     2401 gccct
//
LOCUS       DASUBI010000126         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_691062, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000126 DASUBI010000000
VERSION     DASUBI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691062"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFU72_02385"
     CDS             <1..813
                     /locus_tag="VFU72_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEU5422363.1"
                     /translation="RWRQRKVDLEPGHGLELVVLLVATIYAVILPLKHTISLVDLVVL
                     GSIFAFYIWRLSRLPAEPPHLVGPAETIGALPAVTRRLVTAALALGAAMAILAVAEPF
                     AEALVHTGTEFGIDEFLLVQWLAPLASEAPEFVVVSIFAWRGASGAAMGTLVSSKINQ
                     WTLLVGMLPLIYGISLGAANPLPLGVRQQHELLLTVAQTFFAVALMLDLRLHLYGAAM
                     LFGLFVAGFIWPDHHVALAGVYIALAVIALVVQRQGIREALRHVRGMFGQAT"
     gene            complement(898..2010)
                     /locus_tag="VFU72_02390"
     CDS             complement(898..2010)
                     /locus_tag="VFU72_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5422364.1"
                     /translation="MPSDPTSDQSVARDGARATSGARPRRRLRTVHKLGLLLALATII
                     LLVSSLLGLHPFGWIADVVTNIRRIPPRYIALACALKALESVLTAISWRNVLRAAFPE
                     SAVPFRVVFGAYQGGVGINAVTPANAGSWVMLGLFRVSIRGASIASLVAAALVQGLFF
                     SLLAVAPWLVLMVGDPNAVRAVIESAGRLAVTLFSHPAALGILTAVALLLALILARWL
                     RGRLRNLWQQILTGGAVLRTPRRYVTGVAAPQLASYLCRLGYTATFMAAFDIPVTLRT
                     VFLVVASGSIANGVQITPGGVGSTEAILVVALRGYASASDVTAFSLAKTSILTIWNLL
                     FGFSALSWGFGLARAGRILRNRHRISAQTRAQSADR"
BASE COUNT          339 a          649 c          737 g          335 t
ORIGIN      
        1 cgctggcgtc agcggaaggt cgatctggag ccagggcatg ggctggagct ggtcgtcctg
       61 ctggtcgcga ccatctacgc ggtcattctt ccgctgaagc acactatctc gctggtcgat
      121 ctggtggtgc tgggcagcat cttcgcgttc tacatctggc gcctctcgcg gcttcccgcc
      181 gaaccgcccc acctggttgg acccgccgag acgatcggcg ccttgcccgc tgtcacacgc
      241 cggctcgtga ccgccgcgct cgcgctcggt gccgcgatgg cgattctggc ggtggccgag
      301 ccatttgccg aggcgctggt ccatacgggc acggagttcg ggatcgacga gtttctgctg
      361 gtccagtggc tggcgccgct cgcttcggaa gcgcccgagt tcgtggtcgt cagcatcttt
      421 gcctggcgtg gcgccagcgg ggcggcgatg ggcacgctgg tctcttccaa gatcaaccag
      481 tggacgctgt tggtggggat gctgcccctg atctacggca tctcgctggg cgccgccaac
      541 cccctgccgc tcggcgtgcg acagcagcac gagctgctgc tgaccgtggc gcagacattc
      601 ttcgcggtgg cgctgatgct cgacttgcgg ttgcatttgt acggcgccgc catgttgttc
      661 gggctgttcg tcgccggatt catctggccg gatcatcacg tcgcactggc cggggtctac
      721 atcgccctgg ccgtgatcgc actggtcgtc caacgccagg ggattcgcga ggcgttgcgc
      781 cacgttcgcg ggatgttcgg tcaggctacc tgagcgggga gcggccgccg gagtctgaat
      841 ccggacggtc tcgatccgat acggatgggc gttgccgttc aggtgatgcg gcgccggcta
      901 ccgatcagcg ctctgggcac gggtctgggc cgagattcgg tggcggttgc ggaggatcct
      961 gccggctcgt gcgaggccga agccccacga cagtgctgag aagccgaaca agagattcca
     1021 gatcgtcaaa atcgatgtct tggccagcga gaacgcggtg acgtcgctcg cgctggcgta
     1081 gccgcgcagc gcgaccacca gaatggcctc ggtcgagccg acaccaccgg gcgtgatctg
     1141 gacgccgttg gcgatcgagc cggaggcgac caccagaaag accgtgcgca gcgtcaccgg
     1201 gatgtcgaag gccgccatga acgtggcggt atagcccagg cggcagagat acgatgcgag
     1261 ctgaggtgcc gcgacgccgg tcacatagcg tcgtggcgtg cgcagcaccg cgccgccggt
     1321 caggatctgc tgccagaggt tgcgtagccg tccccgcagc cagcgcgcga ggatgagtgc
     1381 caagagcagg gcaacggccg tgagaatccc cagagcagcc gggtgggaga acagggtcac
     1441 ggccagacgc ccagcactct cgatcaccgc ccgtaccgcg tttggatcgc cgaccatgag
     1501 caccagccag ggggcaacgg ccagcagcga gaagaacagt ccctgaacca gcgccgccgc
     1561 gacgagcgag gcaatgctcg cgccgcggat gctgacgcgg aaaaggccca gcatcaccca
     1621 ggatccggcg ttggccggtg tgaccgcgtt gatgccgacg ccgccctggt aggcgccgaa
     1681 gacgacgcga aacggaaccg cagactcggg gaaggcggcg cgaaggacgt ttcgccagga
     1741 gatcgcggtc agtacggatt cgagggcttt gagcgcgcag gccagcgcga tatagcgcgg
     1801 cgggatgcgg cggatattgg tcacgacgtc ggcgatccag ccgaatgggt gcaggccaag
     1861 gagagagctg accaggagaa tgatcgtggc caaagcgagc agcaaaccca gcttgtgcac
     1921 ggtgcgcagg cgacgacgcg ggcgggcccc cgatgtggcc cgggccccgt cgcgagcgac
     1981 gctctggtca ctggttgggt cgctgggcat tgatcggctt cctcgccgag agggccaccc
     2041 gccgcgcggt tcgccggggt
//
LOCUS       DASUBI010000127         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_691055, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000127 DASUBI010000000
VERSION     DASUBI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691055"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..772)
                     /locus_tag="VFU72_02395"
     CDS             complement(167..772)
                     /locus_tag="VFU72_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422365.1"
                     /translation="MSDKNEPNLPRIPRRSDAARHANLLFGSVEDLLADLIERGAPDG
                     RVVRIERAVRNNVRQTGGSATLGVAVTARREDEVLSCWVVVSRLSLDPWGQPTDRTRA
                     SALTARHHDAQRIIGALFADAGFDVRLGLYRLPDDCYRFAATCAALDQQAAAAQAQPG
                     TDQPRPSGMDRPSTAEDLTSPDDPNGEHTPQHLDGEAEDVH"
     gene            complement(1255..2490)
                     /locus_tag="VFU72_02400"
     CDS             complement(1255..2490)
                     /locus_tag="VFU72_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEU5422366.1"
                     /translation="MTPSERTLPSLPRSYNASRGAQRLTPEELDALKRAHPIEQVAAA
                     YDLSLTRVGAHLVGPCPLHQETRPSFTVYPQSGTFYCFGCRQGGDVIELVRRLEGVGF
                     VAAVERLAGPQLARLIPHRSPRQPVASAAAPSEGRGSNGAHEDDRIRRLLAARASPAG
                     QAALDLATWVYHQTLLQSAPAQAYLAGRGIGYPVARGCRVGYCTGDRLVPALRHQGIP
                     LEAAWAVGLLVGRDGRERFAGRLTIPEVRGGHTVWLTGRLLDPRAMDDAPRYMSLPGP
                     RPLLGAETIAGHPHVVGVEGSMDWLTLVGWGLPGFAALGGALSVEAFAALAHTRTVYL
                     AVDRDGPGQEAARALAARLDGRARRVLLPDGVKDVNELGQRADGRDQFRACLLRAAHP
                     IEPASPNDPASGASGEEAA"
BASE COUNT          458 a         1013 c         1147 g          497 t
ORIGIN      
        1 tcggccacgg accagcagta ggtcgcgcag gtagcgctgc cagcggtgcg tgcgccacca
       61 gggcgccggc gcaggtccag gcagctgggg atgctgcgcg cgggcagcgg ccatctgcgt
      121 caggggtgcg gcgtcgcgcc catccgactg accccggcta atgggcctag tgcacatctt
      181 ctgcctcccc gtccaggtgc tgaggcgtat gctcgccgtt tggatcatcg ggggacgtta
      241 gatcctcagc cgtacttggc cgatccattc ctgatgggcg cggttggtcg gtgcctggct
      301 gcgcttgcgc tgccgcagcc tgctggtcca gcgctgcgca ggtggccgcg aagcggtagc
      361 agtcgtctgg gagccggtag aggccgaggc ggacgtcgaa gccggcatcg gcgaagagcg
      421 caccgatgat gcgctgcgcg tcgtggtggc gcgccgtcag cgcgctggcc cttgtccgat
      481 cggtgggctg gccccaggga tcgagtgaga gccggctcac gacgacccag caggacagca
      541 cttcgtcctc gcggcgcgcc gtcacggcga caccgagggt ggccgagcca ccggtctggc
      601 gtacgttgtt gcggacagcg cgctcgatgc gcacgacgcg gccatctggg gcgccgcgct
      661 cgatcaggtc agccaacaga tcttcgaccg agccaaacag caggttggcg tggcgcgccg
      721 cgtctgagcg ccgggggatg cggggcaggt tcggttcgtt cttgtcactc atggtcgtat
      781 gctcctggga aacagaaggg gcagtcggcc taagccgact gcccgtggat tcgacaaggt
      841 ttcgcgcggt gtgggatacg gacatccgca gccgcagcgg cgatggtgca tgcacggatc
      901 gttgccgaga ctgcccggcc tatgcggttt tgcggagtgg tgggtccacc gacgtggatg
      961 ggcctgcctg ttgccgaacc gctgcgcccg gcggcggctc gtcgtcgggt ggcgcatgcg
     1021 ggtcgccggc atgtgcgggc cggaggactt ccacacggcg gacgtagccc gtccgcacat
     1081 cgcgggcata cacggtctgg cgcatccagc cgagcgatgc gtcgccacct gggaccggcg
     1141 gcaacagccg gagcaacagg tggcggcgcg ggtacggagg acgcgcgggt ggtcgatgct
     1201 gtctcggctg gggaatgtct gccgcgaggg caggcagagg aggttgctgc tccatcacgc
     1261 ggcctcctct cccgacgctc ccgacgccgg gtcgtttggc gaggccggtt cgatggggtg
     1321 cgccgcgcgc agcaggcagg cgcggaactg gtcccggccg tcggctcgct gccccagctc
     1381 gttcacgtcc ttgaccccgt ccggcagcaa cacccggcgg gcgcgtccgt ccaatcgcgc
     1441 agcaagcgcc cgtgccgcct cctggccggg tccgtcccga tcaacggcga ggtagaccgt
     1501 gcgcgtgtgc gcgagcgcag cgaacgcctc aactgacagg gctcctccca gcgcggcgaa
     1561 gccgggcagg ccccacccga ccagagtgag ccagtccatg ctcccctcca cgccgaccac
     1621 atgtggatgg ccggcgatgg tttccgcgcc gagcagtggg cgggggcccg gcaggctcat
     1681 gtagcgtggg gcgtcgtcca tggctcgggg gtcgaggagg cgcccggtga gccagacggt
     1741 gtggccaccg cggacctccg ggatggtgag gcggcccgcg aagcgctcgc gcccgtcgcg
     1801 gccgacgagc agccccacgg cccatgccgc ctccaggggt atcccctggt gacggagcgc
     1861 cgggaccagc ctatcaccgg tgcagtagcc aacgcggcac ccccgtgcga ccgggtatcc
     1921 gatcccacgt ccggccaagt aggcttgggc gggggccgat tggagcagcg tctggtggta
     1981 gacccacgtc gcgaggtcga gtgccgcctg cccggctggc gacgcgcggg ccgccaggag
     2041 gcgccgaatc cgatcgtcct cgtgcgcccc gttggatccc cggccctctg aaggtgcggc
     2101 ggccgaggca actggttggc gcggcgagcg atgcggtatg agccgcgcga gctggggacc
     2161 ggccagccgc tccacggcgg ccacgaagcc gacgccctcc agccggcgga ccagctcgat
     2221 gacgtcgccg ccttgcctgc agccgaaaca gtagaaggtg cccgactggg gatacaccgt
     2281 gaagctcggg cgcgtttcct ggtgaagcgg gcagggcccg acgaggtggg cgccgacccg
     2341 ggtcagggac agatcgtacg ctgccgcgac ctgctcaatg gggtgggcgc gcttgagcgc
     2401 atccagctcc tccggcgtga gtcgttgcgc tccacgagag gcgttgtagc tccgaggcag
     2461 tgacggtagt gtacgctccg agggcgtcat cgttcacctc cttgagcctg ccgagcctgg
     2521 gccccggcgc ggaacgtaag gaggtgacca tgccggccgc tggcgaccct gttgggcttg
     2581 acttcgggtg gcgcgcatgg tactactcga tgcggcgagt gtgctgcgga tctactgggt
     2641 ttcgtgtgga aacggggctt cagtcgggtc caccgcatcg cgcaacgtct gcccgtggtc
     2701 gtctgactca cgacgggggc agaactcaag agccgcccgg attgatggtc cgtggcggct
     2761 ctttggtgtc tgttgcgggg actcgtggtg tcgactcgca tgggtcgcgg tggtagtaat
     2821 ggtggtggcc acggcgactg agacgccgat gaccatttcg tcatgcaccg atcggtttcg
     2881 tttggcatct acgaatctcc tggactgttg gactgtgcaa cgacaggttc agccggcata
     2941 ccgggctcga cctgatggat agcccgattg cctgcagggc atacaggttc agcgggcgga
     3001 ccccgcgcac gcggcgtgga ccgctcgtac ggcccagtcg cagcaggcaa tgtcagacga
     3061 cgaaccgtca cggaatcgcc cctttcatga acagcagcgc gacgaacagc aacgc
//
LOCUS       DASUBI010000128         9943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_636960, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000128 DASUBI010000000
VERSION     DASUBI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636960"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..587
                     /locus_tag="VFU72_02405"
     CDS             279..587
                     /locus_tag="VFU72_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU5422367.1"
                     /translation="MHAHINQFTLKSGGALPPEATDRWRTLIERQPGFQGFLSMRDQE
                     HENQLTIVTLWESAEAAHQWGQNPDFVKIRDSEIQPAISDWTITHATVEAADLRKTSV
                     "
     gene            complement(872..2059)
                     /locus_tag="VFU72_02410"
     CDS             complement(872..2059)
                     /locus_tag="VFU72_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5422368.1"
                     /translation="MPTIEAIRDMLPAVSDTVYLNTGTCGPLPKVAYQAMVEELGHDL
                     GKARIDASHFPHLGELRMAVRQAVAAAIGADPGEIAVTNSTTDGMYVAIVGYRWQRGD
                     ELILSNIEHPGGLVPSFLARRRNGLRTRVADIGLGGGGTDKVVSAFERLITPRTRMIV
                     ISHVSYTTGAVLPVKEIVEMAHSHDVLVVVDGAQGYGALSLDMHALGVDFYACPGQKW
                     MCGPDGTGALYARASSVGEVEQTFVSGGTIRESLDYYGGSVAPALGAARFDTAGRSTV
                     QMTGQVTATRWVTEDVGLDWATKRIAELAGTTYAALEKLNGISMVTPREALGGLIAFN
                     LEGISGEELSHRLADEHNVTIRHVTRYINNPPAARVSVGFYNDESDIQRLIEGIQAVQ
                     KTL"
     gene            complement(2096..3280)
                     /locus_tag="VFU72_02415"
     CDS             complement(2096..3280)
                     /locus_tag="VFU72_02415"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5422369.1"
                     /translation="MEAALAGITVIDFSRHLAGPFAAMTLGDLGADVLKIEAPGRGDD
                     TRGYLPFWNGVSCYYLSANRNKRSLTLNLQAPEGQAIVRRLAAEADVLIENFRPGTME
                     RWGLGEADLRALNPRLIYCAISAVGSEGPDRERAGVDLLMQAYAGLMSITGYPDQPPV
                     RVGTSAVDLTAGANAVQGILAALYVRERTGKGQRVESSLLEGQVSWLTYHAVSYFGTG
                     QVPGRLGSAHASLAPYGAYPTSDGFLVLALASDALWRRFCKAVGRPELAEDARFARNE
                     ARMANRPALEESVSEILAARRAAEWSTLFDAEGIPASTVNSLDTVLNLPQVLHREMVV
                     PLPRDDIPDLKLPGVAVKLSETPGTLRRPPPKLGEHNDEVLAGLGYQPEEIARLHREG
                     VI"
     gene            complement(3293..4057)
                     /locus_tag="VFU72_02420"
     CDS             complement(3293..4057)
                     /locus_tag="VFU72_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5422370.1"
                     /translation="MAQESPVLVETGPEIAVLTLNRPRVRNAVNLAMLEALERALAEL
                     ETDPPRAVILRASAPGFSAGADLKESREQPPEFAHTRVVTMHRVLGKLRRFPAPVISA
                     IDGVCAGLGCELAISGDLRLASPASRLGYPEPKVAVPSPAHHLVHLIGLSRAQDMLLT
                     ARWVEAEEAARIGLVTRVVDDVERAARALAAQVAELAPRAQRLSKENLALSTRAGLEA
                     AVAHHIAGVSGAAYTADRREALAAFAEKRPPRFSGA"
     gene            complement(4309..5133)
                     /locus_tag="VFU72_02425"
     CDS             complement(4309..5133)
                     /locus_tag="VFU72_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422371.1"
                     /translation="MNVTTSDLKQLVETALRNYENYAPPAEDAESASLAIPDPLGYAL
                     GWIVADAIVQDRYLRAALDALPICHPENGWDRIVITRRVTCLLCQEEPADWFGTLMLT
                     GEDAPIYISPEGKELPLGTLLRQDPEAAVARVLELCTLSDLPEGDHANCWHARAVRYP
                     DLYHAVTRIIAEHPGVTALREMYVDEEQIDGAFHPIYLMTGALSKGLEYGWFAIENAD
                     YTAFFRVTGEQSVYFTDNGTWSTVGKQLTEEDNEGIRRRLLSWLRIEGKPDRATLD"
     gene            5316..5846
                     /locus_tag="VFU72_02430"
     CDS             5316..5846
                     /locus_tag="VFU72_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422372.1"
                     /translation="MTTPTSERAEAAARGLERLDLHYIAYVPSNTIAPIINHFRALAT
                     DSAPRAFTVAREEEAVGIIGAVSLAGRRGAIIMQDNGFGNALTALATFAVAYHRPLLI
                     VANTRGGLGEYNSMIHTISAAAPALLREIDIPVFTLDRRNAAADWEATIHEAGRHAQM
                     THRPVVVLMEFWGGQP"
     gene            5846..6439
                     /locus_tag="VFU72_02435"
     CDS             5846..6439
                     /locus_tag="VFU72_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5422373.1"
                     /translation="MKRIEALRVLAELTPGLPVVVTLAATSRELAAVADRDSHLYLLD
                     SMGLAPSVATGLALAIETTPVSRVVALEGDGSLLMNFGALATIGYLRPERLVLVVLDN
                     GVYASTADVPTYTQRLDLAPIASAIGLAAWTAIDEETLRTAMRQALAEPGPHFVHVRI
                     EPGNAPGAPLLLADPVVLAARFEGWLRRAMAGTGVEV"
     gene            6429..7217
                     /locus_tag="VFU72_02440"
     CDS             6429..7217
                     /locus_tag="VFU72_02440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422374.1"
                     /translation="MRSDGTGRSYLVDQQYRGGDNLSARMGLHERFSVNPLGWQRWVF
                     EQLALPDGARVLEVGCGLCALWTQNLDRLSGSWSITLADLSAGMLADARRGLGPDAER
                     FRWLVTDAQALPFADGAFDAVIANHMLYHVPDRSAAIAEFRRVLNDGGRLHAATNGLA
                     HMRELLPLLPAGSPTNPAGRFGLENGAAQLATHFSHVELRRYPDALEVTESAPLLAYL
                     QSIRAGDEFAPSEHAHLRRVIEHEITRRGAFYVTKDVGLFCCNL"
     gene            7429..9459
                     /locus_tag="VFU72_02445"
     CDS             7429..9459
                     /locus_tag="VFU72_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5422375.1"
                     /translation="MGEADRTPRENGGADRPRETMRGRRPGDRYVRVIKSTELPVSPR
                     SQRYVVAPPTEATSALGRLYARLHRLLIGRPIESAQAIHERLTKTKALAVFSSDALSS
                     TAYATEEILLLLVVAGAAAYRMVIPIGVAIAALLAIVVFSYRQTIHAYPQGGGSYIVT
                     KDNLGRKPSLMAASALLTGYTLTVAVSISAGVAALTSASETLLPYRVWLAVGAIVLMM
                     VANLRGVRESGTIFAVPTYLFIGAMVVLLLLGLAAWAGVGLTPHPVHSPLPAVAAPLT
                     AFVVLRAFAAGCAALTGVEAIADGVPAFKEPQAKNAAQTLVWMGVILGTLFLGVTFLT
                     HHFHLAPAADETLVSQLTRTIVGRTPFYYLIQAATAAILVLGANTAFADFPRLSYFLA
                     RDGFMPHQFMARGDRLAFSTGIVVLSGAAALLVISFKAEVSSLIPLYAVGVFVAFTMS
                     QSSMTFRWWRSPRSRQRTLGLAINGLGATTTLVVVLINITTRFFDGAWMVLVILPVFI
                     VVMLGIRRHYDNVAYQLKLSESEGQRQTVDRTLSGESAAVVPMSAIDRASLRAIDYAR
                     RITSNVTAVHVTDDPQEARELQTRWDELDLDVPLVIIESPYREIVGPLVNYIEELHRQ
                     TKGLTITVVLPEFVPAHLWELLLHNQTALRLKFALWTHPDVVVINVPYHLKR"
BASE COUNT         1489 a         3477 c         3406 g         1571 t
ORIGIN      
        1 cgtagcgacc cagacggtat cttgatccag tgtggcgatc ggccactgct ggtcgtcgcc
       61 gctgaaccgg tcgagcccgg ggtcgacgcg cgtctggcgg ccgaagcgag ctgaccgaag
      121 ccggccgccg gctatcgaat cggagcagta ccggagccgg tcggttagcc ccataatcgg
      181 gttgcacgca tacgccgggg gaagggagga atcgacacga ggacaacgcg gggcgccagc
      241 ggtcggaagt agacgattca agccgaaagg agtgcgagat gcacgcccat atcaaccagt
      301 tcacgctcaa atccggtggc gctctgccgc cagaggccac ggatcgctgg cgtacgctca
      361 tcgaacggca gccggggttc cagggattcc tctcgatgcg cgaccaggag cacgagaatc
      421 agttaaccat cgtgaccctc tgggagtccg ccgaagcggc gcatcagtgg ggacagaacc
      481 ctgacttcgt gaagatccgc gacagcgaga tccagcccgc catcagcgat tggacgatca
      541 cgcacgcgac ggttgaggcc gcggacctgc gcaagacctc cgtctgacct ggcaccggca
      601 gaggccgatg accccgagcg cttgactgcg aaggagagcg gatccacgca gagcgattcg
      661 caaagcgtgc gggcggcgca gcgagttccc cgtaggggca gacctccgtg tctgccctcg
      721 atgggttatc gccgagcccc gtcgttccgg ccgctggtgc cccatgtccc cgtttccgag
      781 tcgtaggaca ggaagggcag acacgggggt ctgcccctac agcctaaaac cctggtgccg
      841 accatgcgtg cgacggcggc gactcccgcg gctacagcgt cttctgcacc gcctgaatac
      901 cctcgatcag gcgctggatg tccgactcgt cgttgtagaa gccgaccgag acgcgcgcgg
      961 cgggcgggtt gttgatgtag cgcgtgacgt gccggatggt gacgttgtgc tcgtcggcca
     1021 gccgatgcga tagctcctcc ccggagatac cctcaaggtt gaaggcgatc agcccgccca
     1081 gcgcctcgcg cggggtgacc atgctgatgc cgttcagctt ctccagcgcc gcgtaggtcg
     1141 tgcccgccag ctcggcgatg cgcttcgtcg cccagtcgag ccccacgtct tcggttaccc
     1201 agcgggtggc agtcacctgg ccggtcatct gcaccgtgct gcgcccggcc gtgtcgaagc
     1261 gcgccgcgcc gagtgccgga gccaccgagc cgccgtagta gtcgagcgac tcgcggatcg
     1321 tgccgccgct gacgaaggtc tgctcgacct cgccgacgct ggaggcgcgg gcgtagagcg
     1381 cgccggtgcc gtccggcccg cacatccact tctgtcccgg gcaggcgtag aagtcgacgc
     1441 cgagagcatg catgtcgagc gacaacgcgc cgtagccctg cgcgccgtcg accaccacca
     1501 gcacgtcgtg gctgtgggcc atctcgacga tctccttgac cggcaggacc gcgccggtgg
     1561 tgtacgagac gtgcgagatg acgatcatgc gcgtgcgcgg cgtgatcagg cgctcgaagg
     1621 cggacaccac cttgtcggtg ccgccgccgc cgagcccgat gtcggccacg cgcgtgcgca
     1681 ggccgttgcg gcgccgcgcg agaaaggaag gcaccagccc gccggggtgc tcgatgttcg
     1741 agaggatcag ttcgtcgccc cgctgccagc gatagccgac gatggcgacg tacatgccgt
     1801 cggtggtgct gttggtcacc gcgatctcgc cggggtcggc gccgatggcc gccgccacgg
     1861 cttggcgcac cgccatacgc agctccccca ggtgggggaa gtgcgaggcg tcgatgcgcg
     1921 ccttgccaag atcgtgaccc agctcctcga ccatcgcctg gtaagcgacc ttcggcagcg
     1981 ggccgcaggt gccggtgttg agatagaccg tgtcggacac cgccggcagc atgtcgcgga
     2041 tggcctcaat cgtgggcatt gcccctcctc cgataggtaa accacaaccg acgcatcaga
     2101 tcactccctc acgatgcagg cgcgcgatct cctccggctg gtagcccagc ccggccagca
     2161 cctcgtcgtt gtgctcgccg agcttcggcg gcgggcggcg cagcgtaccc ggtgtctcag
     2221 agagcttgac ggcgacgccc ggcaatttca gatcggggat gtcgtcgcgc ggcagcggca
     2281 cgaccatctc ccggtgcagc acctgcggca ggttgagcac ggtgtcgagg ctattgaccg
     2341 tgctggccgg tattccctcg gcgtcgaaga gcgtactcca ctcggcggcg cggcgagccg
     2401 ccagaatctc gctgaccgac tcttccagcg cgggccggtt ggccatgcgc gcctcgttgc
     2461 gcgcgaagcg cgcatcctcg gccagctccg ggcgcccgac ggccttgcag aagcgccgcc
     2521 agagggcgtc gctggcgagg gccagcacca agaagccgtc actggtcggg tatgcgccgt
     2581 agggcgccag gctggcgtgc gccgagccga gccggcccgg cacctgtccg gtgccgaagt
     2641 aggagacggc gtggtaggtc agccaggaga cttgcccctc caggagcgag gattcgacgc
     2701 gctggccctt gccggtccgc tcgcgtacgt acagcgcggc caggatcccc tgcaccgcgt
     2761 tggcgccggc ggtgaggtcg accgccgaag tgccgacgcg caccggcggc tggtcggggt
     2821 agccggtgat gctcatcagc ccggcgtagg cctgcatcag gagatcgacg ccggcacgct
     2881 cacgatccgg tccctcgctg ccgaccgccg agatggcgca gtagatcaga cgcggattga
     2941 gggcgcgcag gtcggcctcg cccagccccc agcgctccat cgtgccggga cggaagttct
     3001 cgatcagcac gtcggcctcg gcggccagcc tgcgcacgat cgcctggcct tcgggcgctt
     3061 gcaggttgag cgtcaggctg cgcttgtttc gattggcgct gagatagtag caactgacgc
     3121 cgttccagaa ggggaggtag ccgcgcgtgt cgtcgccgcg gcccggcgcc tcgatcttga
     3181 gcacatcggc gcccaggtcg cccagcgtca tggcggcgaa cggcccggcc agatgccggc
     3241 tgaagtcgat gaccgtgatc ccggccagcg ccgcctccat caaacccact ctctaggcgc
     3301 cgctgaagcg cggcgggcgc ttctcggcga aggccgccag cgcctcgcga cgatcggcgg
     3361 tgtaggcggc gccgctgacc ccggcgatgt ggtgcgccac cgccgcctct agaccagcgc
     3421 gcgtgctgag tgccaggttc tccttgctca gtcgctgcgc gcgcggcgcc agctcggcca
     3481 cctgggcggc gagtgcccgc gccgcgcgct cgacatcgtc gaccacccgg gtcaccagcc
     3541 cgatgcgcgc cgcctcctcg gcctcgaccc agcgcgcggt cagcagcatg tcctgcgccc
     3601 gagacaaacc gatcaagtgg acgagatgat gcgcggggga gggcacggcg accttcggct
     3661 ccggatagcc caggcgcgag gccgggctgg cgaggcgcag gtcgcccgag atggccagct
     3721 cgcagccgag cccggcgcag acgccgtcga tggcgctgat gaccggcgcc gggaagcgcc
     3781 gcagcttgcc gagcacccgg tgcatggtga cgacgcgtgt gtgcgcgaac tcgggcggct
     3841 gctcgcgcga ctctttgaga tcggcgcccg cggagaagcc gggcgcgctg gcgcgcagga
     3901 tcacggcgcg gggtggatcg gtctccagct cggcgagcgc ccgctccagc gcctccagca
     3961 tggcgaggtt gacggcgttg cggacacgcg gacggttgag ggtgaggacc gcgatctccg
     4021 ggccggtctc gaccagcacg ggactctctt gcgccacgca ccctcctcgc cagacgccgc
     4081 ctcgcccgcg ctaggctacc gccgttgggg gatgcgcgca agtttgggga aggccctcac
     4141 cccaaccccg gggcgccctc accccaaccc ctctcccctg cgggggcgag gggctacgaa
     4201 ggcttggcct aaccggctcc ctctcccgcg cacgggagag ggcggggggt gatggctccc
     4261 cgtggcgggg ggatgagggc tcccctgtgg cgggggggtg agggcccttc agtcgagcgt
     4321 cgcgcgatcc ggcttgccct cgatgcgcag ccaggagagg aggcggcggc ggatgccctc
     4381 gttgtcctcc tcggtcagtt gcttgccgac ggtcgaccag gttccgttgt ccgtgaagta
     4441 gaccgactgc tcgccggtga cgcggaagaa cgccgtgtag tcggcattct cgatcgcgaa
     4501 ccagccgtac tcgagcccct tggagagcgc gccggtcatc aggtagatcg ggtggaaggc
     4561 gccgtcgatc tgctcctcgt cgacgtacat ctcgcgcagc gcggtgacgc cggggtgctc
     4621 ggcgatgatg cgcgtgaccg cgtggtagag gtcggggtag cgcacggcgc gcgcgtgcca
     4681 gcagttcgcg tgatcaccct cgggcaggtc ggagagcgtg cacagctcca gcaccctggc
     4741 gacggccgcc tcggggtcct ggcgcagcag cgtgccgagc ggcagctctt tgccctccgg
     4801 cgagatgtag atcggcgcgt cctcgccggt cagcatcagc gtgccgaacc agtcggccgg
     4861 ctcctcctga cagaggaggc aggtgacgcg gcgcgtaatg acgatgcggt cccagccgtt
     4921 ctccggatga cagatcggga gagcgtcgag cgcggcgcgc aggtaacggt cctgcacgat
     4981 cgcatccgcc acgatccagc cgagcgcata gcccagcgga tcgggaatcg cgagcgaggc
     5041 gctctcggca tcctcggcgg gcggcgcgta gttctcgtaa ttgcgtaggg cggtctcgac
     5101 gagctgcttg aggtccgagg tcgtaacgtt catcttctcc ccatccagtg tgcggcactc
     5161 gggtccaggc gggctcccac ccggtcgcgt cgactcctcg cctgggccgt cgcactaatt
     5221 gtatgcggcg cggcagtccg aggccaaagc tgctatcctg gccgcaccgg gcggcgcgcc
     5281 cggcggggcc gagcaatcgt ggatgagggt acagtgtgac gacgccgacg agcgagcggg
     5341 ccgaagcggc ggcgcggggt ctggagcggc tagacctcca ctacatcgcc tacgtcccct
     5401 ccaacactat cgcgccgatc atcaaccact tccgtgcgct ggccacggac agcgcgccgc
     5461 gcgcttttac ggtggcgcgc gaggaggagg cggtcggcat catcggcgcg gtcagcctgg
     5521 ccgggcggcg cggggcgatc atcatgcagg acaacggctt cggcaacgcc ctgaccgcgc
     5581 tggcgacctt cgccgtggcc taccaccgcc cgctgctgat cgtggccaat acgcgcggcg
     5641 gtctcggcga gtacaactcg atgatccaca ccatcagcgc ggccgcgccg gcgctcctgc
     5701 gcgagatcga catcccggtc ttcaccctgg accggcgcaa cgccgccgcc gactgggagg
     5761 caacgatcca cgaggccggg cgccatgctc agatgaccca ccggccggtg gtggtgctga
     5821 tggagttttg gggagggcag ccctgatgaa gcgcatcgag gcgctgcgcg tgctggccga
     5881 gctgacgccg gggctgccgg tcgtggtcac gctggcggcc accagccgcg agctggccgc
     5941 cgtggccgat cgcgacagcc acctctacct gctcgactcg atgggcctgg cgccctcggt
     6001 ggcgactggg ttggcgctgg cgatcgagac cacgccggtg tcacgcgtgg tggcgttgga
     6061 gggcgacggc tcgctgctga tgaacttcgg cgcgctggcc acgatcggtt acctcagacc
     6121 cgaacggctg gtgctggtgg tgctcgacaa cggcgtctac gcctcgaccg ccgacgtgcc
     6181 gacctacacc cagcggctcg acctcgctcc gatcgcctcg gccatcggcc tggccgcctg
     6241 gaccgccatc gacgaagaaa ccctgcgcac ggccatgcgc caggcgctgg ccgagccggg
     6301 gccgcacttc gtccacgtgc gtatcgagcc gggcaacgcg cccggcgccc cgctcctact
     6361 ggccgacccg gtcgtgctgg ccgcgcgctt cgagggctgg ctccggcgag cgatggcagg
     6421 caccggcgtt gaggtctgac ggcaccggcc gcagctacct ggtcgaccag cagtaccgcg
     6481 gcggcgacaa tctctcggca cgcatggggc tacacgagcg cttcagcgtc aacccgcttg
     6541 gctggcagcg ctgggtcttc gagcagctcg cgcttcctga tggcgcccgc gtcctggaag
     6601 tcggctgcgg cctgtgcgcc ctctggacgc agaacctcga tcgcctgtcc ggctcctggt
     6661 caatcaccct ggccgacctc tcggcgggga tgctggcgga cgcgcggcgc gggcttgggc
     6721 ctgacgcgga gcgctttcgc tggctggtga ccgacgccca ggcgctcccc ttcgcggacg
     6781 gcgccttcga cgccgtcatc gccaaccaca tgctctacca cgtgcccgac cgctccgccg
     6841 ccatcgcgga gttccgccgc gtgctcaacg atggtggacg gctccatgcc gccaccaacg
     6901 gcctggcgca catgcgggaa ctcctacccc tgctccccgc cggcagcccg accaaccccg
     6961 ccggacgctt cgggctggag aacggcgccg cgcaactcgc gacccatttc tcgcacgtcg
     7021 aattgcgccg ctatcccgac gcgctggagg tcaccgagtc cgcgccactc ctcgcctacc
     7081 tccagtcgat acgagccggc gacgagttcg cgccctccga gcacgcacac cttcggcgcg
     7141 tgatcgagca cgagatcacc cggcgcggcg cgttttatgt caccaaggac gtggggttgt
     7201 tctgctgcaa cttgtagcgc ggcactcatt tcaaagcgcg aaccccggcg gcgcgattgg
     7261 tggtaggatc acgcgcgttg aggggtagca gcgcgatggg cttggctctt ctggcgagag
     7321 cgagccgctg gatttcagca ttcagacggg tcgtgggatc gatcattcgc tacccccaca
     7381 catgtcgcca gcggcggcgc tcgccgccgg cacgcgagga acggggttat gggggaggct
     7441 gatcgcaccc cgcgggagaa cggcggcgcg gaccgtcccc gcgagaccat gcggggcagg
     7501 cgccccggcg atcgctacgt gcgcgtcatc aagtcgaccg agctgccggt cagcccgcgc
     7561 tcgcagcgct atgtcgtagc cccgccgacc gaggcgacca gcgccctcgg ccgcctctac
     7621 gcgcggctgc accggctgct gatcggccgg ccgatcgagt cggcccaggc gatccacgag
     7681 cgcttgacca agaccaaggc gctggccgtc ttctcctccg acgccctctc ctcgaccgcc
     7741 tacgccaccg aggagattct gctgctcctg gtggtggccg gggccgccgc ctaccgcatg
     7801 gtcatcccga tcggcgtggc catcgccgcc ctgctggcga tcgtggtctt ctcctaccgg
     7861 cagacgatcc acgcctatcc ccagggcggc ggctcctaca tcgtcaccaa ggacaacctc
     7921 gggcgcaagc cgagcctgat ggctgcttcg gcgctcctga ccggctacac cctgacggtg
     7981 gcggtgagta tctcggccgg ggtggccgcg ctcacctcgg ccagcgagac gctcctgccg
     8041 taccgggtct ggctggcggt gggcgccatc gtgctgatga tggtggccaa cctgcgcggc
     8101 gtgcgcgaga gcggcacgat cttcgccgtg ccgacctacc tcttcatcgg cgccatggtg
     8161 gtgctgctcc ttctcgggct ggccgcctgg gccggcgtcg gcttgacgcc ccacccggtc
     8221 cactccccgc tgccggccgt ggcggcgccg ttgaccgcgt tcgtggtgct gcgcgcgttc
     8281 gcggccggct gcgccgctct gaccggcgtc gaggcgatcg ccgacggcgt gcccgccttc
     8341 aaggagccgc aggccaagaa cgcggcccag acgctggtct ggatgggcgt catcctgggc
     8401 accctcttcc tgggtgtcac cttcctgacg catcacttcc acctggcgcc ggccgccgac
     8461 gagacgctgg tctcccagct cacgcgcacg atcgtcgggc gcaccccctt ctactacctg
     8521 atccaggccg ccaccgccgc catcctggtg ctcggcgcca acaccgcctt cgccgacttc
     8581 ccccggctga gctacttcct ggcgcgcgac ggcttcatgc cgcaccagtt catggcgcgc
     8641 ggcgaccggc tggccttctc gaccggcatc gtcgtgctga gcggcgcggc ggcgctgctg
     8701 gtcatctcat tcaaggccga agtgtcgtcc ctgatcccac tctacgcggt cggcgtcttc
     8761 gtggccttca ccatgtcgca gtcgagcatg accttccgct ggtggcgctc gccgcgtagc
     8821 cgccagcgca cgctcggtct cgcgatcaac gggctcggcg ccaccaccac gctggtagtg
     8881 gtgctgatca acatcaccac gcgcttcttc gacggcgcct ggatggtgct ggtcatcctg
     8941 ccggtcttca tcgtggtgat gctggggatt cgccggcact acgacaacgt ggcctaccaa
     9001 ctgaaactca gcgagagcga ggggcagcgg cagaccgtcg atcgcacact gagcggcgag
     9061 agcgccgccg tggtgccgat gagcgcgatc gaccgcgcct cgctgcgcgc catcgactac
     9121 gcgcgccgga tcaccagcaa cgtgaccgcg gtccacgtca ccgacgatcc gcaggaggcg
     9181 cgcgaactgc agacgcgctg ggacgagctg gacctggatg tgccgctggt gatcatcgag
     9241 tcgccctacc gcgagatcgt cgggccgctg gtgaactaca tcgaggagct gcaccggcag
     9301 accaaggggc tgaccatcac cgtggtgctg ccggagttcg tgccggccca cctctgggag
     9361 ctgctgctgc acaaccagac ggcgctgcgc ctcaagttcg ccctctggac ccaccccgac
     9421 gtggtcgtga tcaacgtgcc gtaccacttg aagcggtgat gggttgatac gttccgtcgc
     9481 ctgcattgga atattcgcca tcgttgaatg ttgggggccg catgacgcgc tgggctaaag
     9541 ccgcagcgct aaccaatcga agcccactga aggggctgaa ctttcaagct ccaggcatgg
     9601 cggcgaaatg cagcttccgc gacagatgca cgtttgagag atccttcgct gcggctcagg
     9661 ttgagaggaa tggagcctga gctcctccgg tcatcctgag gctgcggccg aaggatctct
     9721 cgtgcctttc cccaacgacc catgtttcag gatgatgcac atcaggccca tgccggatcg
     9781 tgccctttga gaaccgtccc gcagctcatc acgctcgccc gccggccggc cacctcggcg
     9841 ggagcgggtc gcgctggagg taggccagga tcagctcgac ggtgtgctcc aggtcctcct
     9901 cggtcaccat ctcgttgggc gagtgggtgt agcgggtggg gaa
//
LOCUS       DASUBI010000129         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_513487, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000129 DASUBI010000000
VERSION     DASUBI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513487"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1706)
                     /locus_tag="VFU72_02450"
     CDS             complement(252..1706)
                     /locus_tag="VFU72_02450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5422376.1"
                     /translation="MTTSERQQATWAPEVRAFSREMLVRHEVPGLALAAAQDGREVFA
                     EGFGVRELGRPEAVTPDTVFGVASVTKSFTALAVMQLFEAGKLALDDPVTRYLPEFRL
                     RDAAHAGAITVHHFLTHAGGLPPLPSRFFAFAQAAAADPHANKKPEWEADHAAFARFE
                     DLMDYLAEREITPLGPPGAQFSYCNEGFVLLGAIVERVSGEPYPDYILRHILEPLGMT
                     RSTFEADTLRGFDDVTTFHTARRENGQREVFPAPYWALSRVWAPAGDLNTTVRDLLRY
                     LEIFRTGGVGGANDGDGAVRLLSPAGIARMTYPHQPTNVPGTFYGYGLQVTPGYHGVT
                     VIQHGGGRKGIAANVLVAPEAGITAAAVANLDAVPAAQVTLAVLNGALGLPVDTKLTE
                     HVEQPASSAPLARYVGNYRGGEGQNYTFSVEDGALRASTAAGSFAARPVGGHDFLLDT
                     PGTETFARFLVRGDEPAWAVAAGSRIIPRWRDEG"
     gene            1867..2250
                     /locus_tag="VFU72_02455"
     CDS             1867..2250
                     /locus_tag="VFU72_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422377.1"
                     /translation="MWYVRRVVKGLGIAYGLTLANLLPLFLIHILLPPVAPLIGGYMA
                     GAVEMLGPNESLALGLLSGALVGLPLPIAYSVFGIMGYLTPLAIYFMAGFSALYCGFT
                     IGGLAWIAGDAARRHQRELAEARGS"
     gene            complement(2492..>3090)
                     /locus_tag="VFU72_02460"
     CDS             complement(2492..>3090)
                     /locus_tag="VFU72_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU5422378.1"
                     /translation="AGTPPQQQQQQNQGENPVQKILREPHQAWPAWIRDSLEILLVVI
                     VVAVIFWLALRTLRRLRKPDDELSVDEVHESVWSADLALAQLRDLLSGLRPRRAGHAQ
                     AARFDLAGEPADVREAYRRVVVLGAREGNERQPGETALDYSARLERVWADLGEPLGDL
                     TGRYLRARYAEESDQEDVRRARLDWRAIAARIVPERRR"
BASE COUNT          422 a         1109 c         1081 g          478 t
ORIGIN      
        1 ccaggcttaa tggtagcccc gaggcgcgcg ccgggctatc gggatcgtgc ggtcaaactc
       61 cgggcttcgg agcacactga gtgccgggtg agcacggatc cttcggctgc gctcaggatg
      121 actggggtag ttgggggcgg acaacgacga tgcgtgtcgt ccgaaaggcc atccccttta
      181 ccctgtcatc ctgagcgcag tcgaaggatc cgtgtcccag gcgacaccca atcctcttga
      241 tagcccgcgc cctacccctc gtcccgccag cgggggatga tccggctgcc ggcggcgacg
      301 gcccaggccg gctcgtcgcc gcgcaccagg aagcgggcga aagtctcggt gccgggcgtg
      361 tcgagcagga agtcgtgccc gccgaccggg cgcgcggcga agctgccggc cgcggtgctt
      421 gcccgcagcg ccccgtcctc gacgctgaag gtgtagttct ggccctcgcc gccgcgatag
      481 ttgccgacat agcgcgcgag cggcgcggac gaggccggct gctcgacgtg ctcggtcagc
      541 ttggtgtcga ccggcaggcc gagcgcgccg ttcaggaccg ccagggtgac ctgagccgca
      601 ggcacggcgt cgaggttggc aaccgcggcg gcggtgatac cggcctcggg cgccaccagc
      661 acgttggccg cgatcccctt gcgtccgccg ccgtgctgga tcacggtcac gccatggtag
      721 ccgggggtga cctgcaaacc gtagccgtag aaggtccccg gcacgttggt cggctggtgc
      781 gggtacgtca tgcgggcgat cccggccggc gagaggaggc gcaccgcgcc gtcgccgtcg
      841 ttggcgcccc caactccgcc cgtgcggaag atctccaggt agcgcagcaa gtctcgcacg
      901 gtggtattga ggtcaccggc cggcgcccag acgcgcgaca gcgcccagta gggcgccggg
      961 aagacctcgc gctgcccgtt ctcgcgcctg gcggtgtgga aggtggtcac gtcgtcgaag
     1021 ccacgcaggg tgtcggcctc gaaggtgctg cgcgtcatcc ccagcggttc caggatgtgc
     1081 cgcaggatgt agtcggggta gggctcgccg gagacgcgct cgacgatggc gccgagcagc
     1141 acgaagccct cgttgcagta gctgaactgc gcgccgggcg gccccagcgg ggtgatctcc
     1201 cgctcggcca ggtagtccat caggtcttcg aagcgcgcga aagcggcgtg gtcagcctcc
     1261 cactcgggct tcttgttggc atgcgggtcc gcggcggccg cctgggcgaa ggcgaagaag
     1321 cgcgagggga gcggcggcag cccacccgcg tgggtcagga agtggtgcac cgtgatcgcg
     1381 ccggcgtgcg cggcatcgcg cagccggaac tcgggcaggt agcgcgtcac cgggtcgtcg
     1441 agcgccagct tgccggcctc gaagagctgc atcaccgcca gcgcggtgaa agacttggtg
     1501 accgaggcca cgccgaagac ggtgtccggc gtcaccgcct ccggccggcc cagctcgcgc
     1561 acgccgaagc cctcggcgaa gacctcgcgc ccgtcctgcg ccgccgccag cgccagcccc
     1621 ggcacctcat gccttaccag catctcgcgt gagaaggcgc gcacctccgg cgcccaggtt
     1681 gcttgctgcc gttcgcttgt cgtcatccct gctgcattcc ttcccgcgcg gtcccgcacc
     1741 tgccgcgaca atgatgcgct tgtggcacgc gcacggcgga cttgtcaacc cgttgcctga
     1801 ggctcacaat ggctggcaag gaaccggtga gccgggcgcg gatgccttcg gtggggaggg
     1861 cacgcgatgt ggtacgtcag gcgggtcgtc aagggcctgg gcatcgccta cggtctgacg
     1921 ctcgccaacc tgctgccgct cttcttgatt catattctcc tgccgccggt cgcgccgctg
     1981 atcgggggct acatggccgg cgcggtcgag atgctcggcc ccaacgagtc gctcgcgctg
     2041 gggctgctct cgggggccct ggtcgggctg ccactaccga tcgcctacag cgtcttcggc
     2101 atcatgggct acctgacccc gctggcgatc tacttcatgg ccggcttcag cgcgctctac
     2161 tgcggcttta ccatcggcgg tctggcctgg atcgccggcg acgcggcccg acgccaccag
     2221 cgcgagctgg ccgaggcgcg ggggagttga cacgcgtcag cgcggcgacg attccttgcg
     2281 gtctctcgta cgccacgcat tttcggcgat gcgccccttt cccctgtagg ggcagacctc
     2341 tgtgtctgcc ctcgcggccc atcaccgggc gttgtcgtcc gccgtccacg ctccccatgg
     2401 ccctgctgcc tcgccgtagg gggaacaggg cggccacggg ggggccgccc ctacagggga
     2461 tcacgcctcg atacctcaaa acgcatgcgt tttaccgccg ccgctccggc acgatgcgcg
     2521 cggcgatggc gcgccagtcg agccgcgccc ggcgcacgtc ctcctggtcg ctctcctcgg
     2581 cgtagcgggc gcgcaggtag cgcccggtca ggtcgcccag cggctcgccc aggtcggccc
     2641 agacgcgctc cagccgcgcg ctgtagtcga gcgcggtctc gcccggctgg cgctcattgc
     2701 cctcgcgcgc ccccagcacc accacccgcc ggtaggcctc gcgcacgtcg gccggctcac
     2761 ccgccaggtc gaaccgcgcc gcctgggcgt gcccggcgcg gcgcggccgc agaccgctga
     2821 gcaggtcgcg gagctgcgcc agcgccaggt cggccgacca gacgctctcg tggacttcat
     2881 cgaccgacag ctcgtcatcc ggcttgcgca ggcggcgcag cgtgcgcagc gccagccaga
     2941 aaatgaccgc caccacgatc actaccagca ggatctccag ggagtcgcgg atccaggccg
     3001 gccaggcctg atgcggctcg cgcagaatct tctgcaccgg attctcgccc tggttctgct
     3061 gctgttgctg ctggggcggc gtgcccgcca
//
LOCUS       DASUBI010000130         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1304043, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000130 DASUBI010000000
VERSION     DASUBI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304043"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFU72_02465"
     CDS             complement(<1..340)
                     /locus_tag="VFU72_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422379.1"
                     /translation="MRRLVVGLIVALAAVAVLPLLAAAADPTVTVTNNNSLGMILTDP
                     AGKTLYIFKKDTPNTSNCYDACAQNWPPLVIKAGQPVAPANLGGDLGTTTRKDGSLQV
                     TFQGMPLYYYF"
     gene            complement(608..1159)
                     /locus_tag="VFU72_02470"
     CDS             complement(608..1159)
                     /locus_tag="VFU72_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5422380.1"
                     /translation="MPVRLPVSLGSILALALGLTLLLGLAACGSGGSSPAAPSAAAVA
                     TQPPGATAGPSPVATGAGAGVATSTAHALPASPAPTGVPTPPPTIAATPSAPVAAEVQ
                     VKIVNFAFEPATITIKPGTTVVWTNTSPTQHTTTSRQPANVWDSGIMDSGAVFRFTFQ
                     QAGEFDYWCALHPEMLGKVIVKP"
     gene            complement(1236..1781)
                     /locus_tag="VFU72_02475"
     CDS             complement(1236..1781)
                     /locus_tag="VFU72_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422381.1"
                     /translation="MKRAWLLAALAALTMLLAAALSAAAADTTITVQMTAQNGSGESG
                     TATLTDLGNGQTKVVVNLTGAPAGVAQPAHIHEGTCSNLNPTPKYPLTSVMDGKSETT
                     VPVSLADLMSENYAINVHKSQAEVQTYFSCGDIPAAAVGQVAPKTGQAGNLGLLSLTG
                     LLALAGGALAGGLVLRRRTAR"
     gene            2041..2736
                     /gene="dhaL"
                     /locus_tag="VFU72_02480"
     CDS             2041..2736
                     /gene="dhaL"
                     /locus_tag="VFU72_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU5422382.1"
                     /translation="MAQRDTAASDGLLGQVPQPVLGRRESIGLGTLARVQAAIRENAD
                     ELSRLDAAIGDGDHGYNLARAFHEAMVAVERLPEETPVAALTATGEALRAIACLASGL
                     YAAGCLAAARALPAERPPSTRDLAAAFEAGVAAIQAEGHAVPGNKTMVDALRPAADAL
                     LAAVAGEATLALALDRAAAAARAGMAATTAQPGVFGRAGKLGNAAIGHLDPGATSAYL
                     IVQALAHAAHALP"
     gene            2846..3499
                     /locus_tag="VFU72_02485"
     CDS             2846..3499
                     /locus_tag="VFU72_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5422383.1"
                     /translation="MSISRWNPWGDMMSLRDAMDELLRESFVLPRPGTASGGFGVALD
                     VRETNDDYVVKAVLPGVKPNEVHVQVKGDTLTISGETKEEEEQGPGQQQPGQQGQSQS
                     QGQTQQTQSQQTQAHGQQGQQPGQQAQRGQWLVRERRFGRFQRTVTLPTPVQADQAKA
                     NFEHGVLTITLPKSQEARARSIPIQAGQGQGQTPAIEAQGQVHGQQSQQGQQGQVHS"
     gene            3614..4216
                     /gene="plsY"
                     /locus_tag="VFU72_02490"
     CDS             3614..4216
                     /gene="plsY"
                     /locus_tag="VFU72_02490"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5422384.1"
                     /translation="MTVLLAGLLTLAAYLLGSIPAGLVISYRASGLDIRTQGSGNIGT
                     ANVYRVAGRRAAALTLLFDACKGLAPVLAARLLGLPLWTIFPIGAAAVLGHNFSFILR
                     GHGGKGIATSIGVIGALAPPAALLAIAVWLLVLLGSRYASLASLLMMAALPALLALLG
                     YPPGDPLFALGLVLLAVYRHRANIARLLAGTEPRFGKKKA"
BASE COUNT          632 a         1462 c         1554 g          592 t
ORIGIN      
        1 tgaaataata gtagagcggc atcccctgga aggtcacctg gaggctgcca tccttgcgcg
       61 tggtcgtgcc caggtcgccg ccgagattgg ccggggcgac cggctggccg gccttgatca
      121 cgagcggcgg ccagttctgc gcgcaggcgt cgtagcagtt gctggtgttg ggggtgtcct
      181 tcttgaagat atagagggtc ttaccggcgg ggtcggtcag aatcatgccc agcgaattgt
      241 tgttggtcac ggtcacggtc gggtccgccg cggcagccag caggggcagc actgccaccg
      301 cggccagcgc cacgatcaaa cccacgacaa gacgacgcat cgcgactacc tccagtaccg
      361 ccggactcgc ccggcggcgc ttctcgattc cggcactctc agctgcagct cgcaggttct
      421 acgcgcgtga acgggaatcg gatcgaagcg cacggccgcg agcagcgccg ggtatttcaa
      481 tgccggcgcg atccggcccg cgctggcggc cggccactgg gtggcgggcg caagcaacag
      541 ctaccggcgg ccgcgcgctg ggtggcgcgg gccggcactg gggctcgacg aggaggcgcg
      601 gccgcggcta gggcttgacg atgaccttgc cgagcatttc gggatggagg gcacaccagt
      661 agtcgaattc accggcctgc tggaaggtga agcggaagac ggcgcccgaa tccatgatgc
      721 cggagtccca gacgttcgcc ggctgccggc tcgtggtggt gtgctgcgtc gggctggtat
      781 tcgtccagac gaccgtcgtg ccgggtttga tcgtgatggt cgcgggctcg aacgcgaagt
      841 tgacgatctt cacctgcacc tcggccgcga ccggggcgga cggtgtcgcg gcgatcgtcg
      901 ggggtggcgt gggtacgcca gtcggcgcgg gcgacgccgg cagcgcgtgc gcggtcgagg
      961 tggccacgcc cgcgcctgcg cccgtggcca ccggcgatgg cccggccgtg gcgcctggag
     1021 gctgggtggc gacggcggcc gcgctgggag cggcggggga gctaccgccc gagccgcagg
     1081 cggcgagacc gagaagcagc gtcaggccga gcgcgagcgc gagaatcgag ccgagcgaaa
     1141 caggtagacg gacaggcatc gcgagtcctc tgaatcctac ggggcgcgag acggtctcgc
     1201 gccccgacat cgcgtcagtc gtacggcgtg ccggtctagc gagccgtccg ccgccgcagc
     1261 accaggccac cggcgagcgc gccgccggcg agcgccagca gacccgtgag cgacaggagg
     1321 ccgagattac ctgcctgacc ggtcttcggc gcgacctgac ccaccgccgc ggccggaata
     1381 tcgccgcagg agaagtaggt ctgcacctca gcctgagact tatgcacgtt gatcgcgtag
     1441 ttctcactca tcaggtcggc gagactcacc gggacggtcg tctccgactt gccgtccatc
     1501 acgctggtca acgggtactt gggggtcggg ttgagattgc tacaggtacc ctcgtggata
     1561 tgggcgggct gtgcgacacc cgccggcgcg ccggtcaaat tgacgacgac cttggtctgg
     1621 ccgttgccca gatcggtgag cgtcgccgtg ccggactcgc ccgaaccgtt ctgtgccgtc
     1681 atctggaccg tgatcgtggt gtcggccgca gcggccgaga gcgcggccgc gagcagcatt
     1741 gtcagtgccg ccagtgccgc gaggagccat gcgcgcttca ttaccctact cccttcacac
     1801 gaaaccctgg atagcccgtc cgccggaggc tcgcccggcc gcgatccgcg ccggtgaccg
     1861 ggcgcggccg cccgctcgcg cggatcgccg cacccggtca agacgtctac gcacggggat
     1921 gcgtatcgga tgaagccgca cggtgcgcgc gcggactggc cggatccgaa cctggaaggc
     1981 gcggcacgct cctggcaata cattcgccag cgtggtgtgg acaacgagag agggcgtgag
     2041 atggctcagc gagacacagc cgcctctgac ggcctgctgg ggcaggtgcc gcagccggta
     2101 ttggggcggc gtgagtcgat cggtctcggc acgctggcgc gggtgcaggc cgccatccgc
     2161 gagaacgcgg acgagctgtc gcggctcgac gcggccatcg gcgacggcga ccacggttac
     2221 aacctggcgc gcgccttcca cgaggcgatg gtcgcggtcg agcgcctgcc cgaggagact
     2281 ccggtcgccg cgctcacggc gaccggggag gcgctgcgcg cgatcgcgtg cctggcgtcc
     2341 gggctgtacg cggcgggctg cctggcggcc gcgcgggcgc ttcccgccga acggccgccg
     2401 agcacgcgcg atctggcggc agcgttcgag gcgggcgtgg cggcgatcca ggccgagggg
     2461 cacgccgtgc cgggcaataa gacgatggtc gacgcgctgc gcccggcggc cgacgccctg
     2521 ctggcggcgg tggccggcga ggcgacgctc gccctggcgc tcgaccgggc ggcggccgcg
     2581 gcgcgggccg gcatggcggc gaccaccgcg cagcccggcg tcttcggccg ggcgggcaag
     2641 ctgggcaacg ccgccatcgg ccacctcgac ccgggcgcga cctcggccta cctcatcgtg
     2701 caggcgctgg cgcacgccgc ccacgccctg ccctgagcgc gctgccggcc gaatcccggc
     2761 atgcaagctg cagagattat cgtgcccaga gtcgtagccg actcgcggga gctatatccg
     2821 tttttcaagg taaggagcca tgcccgtgag catctcgagg tggaatccct ggggcgatat
     2881 gatgagcctg cgggacgcga tggacgagtt gctgcgcgag agtttcgtcc tgccgcgccc
     2941 cggcaccgcg tccggtggct tcggcgtggc gctcgatgtc cgtgagacca acgacgacta
     3001 cgtcgtcaag gcggtactgc ccggcgtgaa gccgaacgag gtccacgtgc aggtcaaggg
     3061 cgacacgctg acgatctcgg gcgagaccaa ggaggaagag gagcagggac ccggccagca
     3121 gcagccaggg cagcagggcc agagccagag ccaggggcag acgcagcaga cccagagcca
     3181 gcagacccag gcgcacggtc agcaggggca gcagccaggg cagcaggccc agcgcggcca
     3241 gtggctggta cgggagcgcc gcttcggccg cttccagcgc accgtcaccc tgccgacgcc
     3301 ggtgcaggcc gatcaggcca aggcgaactt cgagcacggc gtgctgacca tcaccctgcc
     3361 gaagtcgcag gaggctcgag cccgctcgat ccccattcag gcggggcagg gccagggcca
     3421 gaccccggcg atcgaggctc agggccaggt gcacgggcag cagagccagc agggccagca
     3481 gggccaggtc cacagctagc gcgagcgcgc tcgcgagcca gcgccgcggc gcccaccggg
     3541 tgccgcggct cgtgtttaat ggccctggag cgacgcgctc ccaccgtccg catgcaggag
     3601 agcgacaccg ccgatgaccg tgctgctggc cggcctgctc acgctcgccg cttatctact
     3661 cggctcgatc cccgccggcc tggtcatctc ctaccgcgcc tccggcctcg acatccgcac
     3721 ccaggggagc ggcaacatcg gcacggccaa tgtctaccgc gtagccgggc gccgtgccgc
     3781 cgcgctgacc ctcctcttcg acgcctgcaa ggggctggca cccgtgctgg cggcgcgcct
     3841 gctcgggctg ccgctctgga cgatcttccc catcggcgcg gcggcggtcc tcggacacaa
     3901 cttctcgttc atcctgcgcg ggcacggcgg caaggggatc gccacctcga tcggggtgat
     3961 cggcgcgctg gcgcccccgg ccgccctgct ggcgatcgcc gtctggctgc tggtgctcct
     4021 gggcagccgc tacgcctcgc tggcctcgct gctgatgatg gccgcgttgc cggccctgct
     4081 ggcgctgctc ggctacccgc ccggcgaccc gctcttcgcg ctggggctgg tgctgctggc
     4141 ggtctaccgc caccgcgcca acatcgcccg cctgctggcc ggcaccgagc cgcgcttcgg
     4201 caagaagaag gcgtgagcgc ggtccgatcg ccggaaggcg
//
LOCUS       DASUBI010000131         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_78641, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000131 DASUBI010000000
VERSION     DASUBI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78641"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..285
                     /locus_tag="VFU72_02495"
     CDS             58..285
                     /locus_tag="VFU72_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5422385.1"
                     /translation="MSRSALLVMDVQHAIVDRFAASESAAGYIPRLREAIDAAHHAGI
                     PVVYVVIGFREGHPEISPRNKTFSEVFRATS"
     gene            427..1278
                     /locus_tag="VFU72_02500"
     CDS             427..1278
                     /locus_tag="VFU72_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU5422386.1"
                     /translation="MADDPVTFTCALEARRETVDFLARLLHQRRRELGTRSGTRALGP
                     FKHAVLALRWFLDNTRVRQLATDNAISLSTAYDYLHEAIDVLAACAPDIHDALLAAKA
                     GGATHLNLDGTLIRTDRVAMRGPNGADLWWSGKHKHHGGNIQVLSYPDGFPCWVSDVR
                     PGREHDTTCAKKAEDLLPALELYEAEHQMPTLTDLGYIGLSPAIRHPHKKPKGAELTE
                     TQTTYNTVIRGIHAIAERANALLKETFAALQHVSLSPTRIGAITKAALVLLHLEHGRP
                     IPGGYTA"
     gene            1320..2189
                     /locus_tag="VFU72_02505"
     CDS             1320..2189
                     /locus_tag="VFU72_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEU5422387.1"
                     /translation="MTALYVKIDDELVHERWRGRPPLLSDSELVCLAVAQALLEVRSE
                     AKWIRFVGQRLGALFPYRPQRPGYNKRLRAALPLVKRVIRLLARDTDFWLDTVWITDS
                     TPVPCGMSRPTVKRSNLAGWAGYGYCASHSRFFWGLRLYLVCTPSGMPILWALANPKL
                     GEREVLGAMLEVDADLVAEHAGVLLITDKGFAGRAFERDLAAHGVTLLRPSRKDERAR
                     GGEPMLKKVRQLIESVNDTLKGQLDLERHGGQSFEGVAIRVAQRVLALAAAIWHNFHT
                     GQTVSRSLTAYDH"
     gene            complement(2254..>2529)
                     /locus_tag="VFU72_02510"
     CDS             complement(2254..>2529)
                     /locus_tag="VFU72_02510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422388.1"
                     /translation="PRPDWRITCFYVDTRHRGQGVAREALRGAIEQIAQAGGGRTEAI
                     SEVTADRTAHGRFLFSATVELFEEHGFSRVRQVGKHAWIVSRLIEPV"
BASE COUNT          451 a          943 c          768 g          367 t
ORIGIN      
        1 tggtccgcgc cgatcaggca ctcgcccacc cgaccgcgca ccgaaacgag accgaacatg
       61 agtagaagcg cgctcctggt gatggacgtg cagcatgcca tcgtggaccg gttcgccgct
      121 tccgagagcg cggcggggta cattccccgg ctgcgcgagg cgatcgacgc cgcacaccac
      181 gccgggatcc cggtcgtcta cgtggtcatc ggcttccgcg aagggcaccc ggaaatcagc
      241 ccccgcaaca agaccttcag tgaggtcttc cgcgcaacgt cgtgagcaca cgccgtgatg
      301 gccgcgtgag tcgccctggg ccgtcccacg atctgacaca ccgtcatggc ggcacgaaac
      361 ccctggtaga agcaggtctt gacgagagat cagcttcaca ccgaggggtt tcgtgtggct
      421 cctatcatcg cagacgaccc ggtcaccttc acctgcgcgc tcgaagcacg ccgagagacc
      481 gtggacttcc ttgcccggct gctccaccag cggcgacgcg agctcggcac ccgctcgggc
      541 acgcgagcac tcgggccgtt caaacacgcg gtgctcgcgc tgcgctggtt cctcgacaac
      601 acccgcgtcc gccagttggc caccgacaac gcgatcagcc tctccaccgc ctacgactac
      661 ctccacgaag cgatcgacgt gctcgccgcc tgcgccccgg acatacacga cgcgctgctg
      721 gccgccaaag ccggcggcgc cacccatctc aacctcgacg gcaccctgat ccgcaccgac
      781 cgcgtcgcga tgcgcggacc caacggagcc gacctgtggt ggagcgggaa acacaaacac
      841 cacggcggaa acatccaggt cctgtcgtac ccggacgggt tcccctgctg ggtctcggac
      901 gtgcgcccgg gccgcgagca cgacaccacc tgcgcgaaga aagccgaaga cctgctgcca
      961 gcgctcgaac tctacgaggc cgaacatcag atgccgacgc tgaccgacct gggctacatc
     1021 ggcctctcac ccgcgatccg ccacccgcac aaaaagccca agggcgccga gctcaccgag
     1081 acacagacca cgtacaacac ggtcatccgc ggcattcacg ccatagccga gcgagccaac
     1141 gcactgctca aggagacctt cgccgcactc caacacgtca gcctcagccc cacccgcatc
     1201 ggcgcgatca ccaaggccgc actcgtcctc ctccacctcg aacacggccg ccccatccca
     1261 ggcggctaca ccgcgtaacg cagccttacg cggaacacct cactgatctc gagaccctca
     1321 tcacggcact gtacgtgaaa atcgacgacg agttggtgca cgagcgctgg cgtgggcggc
     1381 cgccgttgtt gtccgactcc gaactcgtct gcctcgccgt ggcccaggcg ctgctggaag
     1441 tacgtagcga ggcgaagtgg atccggttcg tcggccagcg cctgggcgcg ctgtttccct
     1501 accggcctca gcgccccggc tacaacaagc gcctgcgggc ggcgctgccg ctggttaaac
     1561 gcgtgatccg gctacttgcg cgggacaccg acttctggtt ggacacggtg tggatcaccg
     1621 attccactcc agtgccgtgt gggatgtccc ggccgacggt caaacgctcg aacctggccg
     1681 gctgggccgg gtacggctac tgcgcgagcc attcgcggtt cttctgggga ctacggctct
     1741 acctggtgtg caccccgtcg ggcatgccga tcctgtgggc cctggccaac ccgaagctcg
     1801 gggaacgcga agtgctcggc gcgatgctgg aggtggacgc cgacctggtc gccgagcacg
     1861 ccggtgtcct gctgatcacg gacaagggct tcgccgggcg cgccttcgaa cgcgacctgg
     1921 ccgcgcacgg ggtaacgctc ctgcgcccct cgcgcaagga cgaacgcgca cgcggcggcg
     1981 aaccgatgct caagaaggtg cgccagctga tcgaatcggt aaacgacacg ctcaaaggcc
     2041 agctcgatct cgagcgccac ggcggccaga gcttcgaggg cgtcgccatc cgcgtcgccc
     2101 aacgcgtcct ggccctggcc gccgcgatct ggcacaactt ccacaccggc cagacagtca
     2161 gccgatcact tactgcctac gaccattagg aactcatcgt ctagccccgc cgaccgaccc
     2221 gcaccgccgg gcccgtccgc caggcccgtc ccgctagacc ggctcgatca gcctgctgac
     2281 gatccacgcg tgcttgccga cctggcgcac ccggctgaat ccgtgctctt cgaagagctc
     2341 gacggtggcg ctgaacaaga accggccatg cgccgtgcgg tccgcggtga cctcggagat
     2401 ggcctccgtc cgtccgccgc cggcctgggc aatctgctcg atcgcgccgc gcagcgcctc
     2461 gcgggccacg ccctgcccgc ggtggcgggt gtcgacgtag aagcaggtga tccgccagtc
     2521 cggccgggg
//
LOCUS       DASUBI010000132         6212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_487244, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000132 DASUBI010000000
VERSION     DASUBI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487244"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VFU72_02515"
     CDS             complement(<1..1773)
                     /locus_tag="VFU72_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5422389.1"
                     /translation="MHNHTPPQDERGTDAPAPARHQQHAHTGHEGHTEQIQHGHDAHA
                     GHAEHIEHAPREAHGHGAQAGHAGHDEHASGAPHASHAGHAGHAGHADVFKRRFWVSL
                     VLSLPVVVFSPMVQDWLGYSAPSFPGSDLIAPILGTIVFLYGGWVFLTGAREELSERK
                     PGMMTLVSMAITVAFVASLATTFGLFGLDFWWELALLIDIMLLGHWLEMRALGQAQGA
                     LSALAALLPDQAERVVDGEVQSVPVADLQVGDVVLVRPGGRVPADGAIVDGQAELDES
                     MLTGESRPIPKGPGDKVVAGTVVAGSSVRVRVEAVGAETALAGIERLVAEAQSSQSRA
                     QRLADRFAGWLFYAAAGAGLVTFVVWVLLGVIDDAVVRTVTVLVIACPHALGLAIPLV
                     VALSTSLAARQGILIRDRMALERMRTIDTVLFDKTGTLTLGSYALSAVVPVSGDEDAV
                     LRLAAAAESDSEHPVAGAIVAAAAERDLALPRAADFRASAGRGVEATVEGQAVAVGGP
                     AMLREQGLTVPPELEARMRPWAARGAGILHVIRDGQVIGALALEDRVRPESRAAIDEL
                     HRRGVRVVMITGDARSVAEAVAKDL"
     gene            complement(2075..2680)
                     /locus_tag="VFU72_02520"
     CDS             complement(2075..2680)
                     /locus_tag="VFU72_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422390.1"
                     /translation="MVFERDNRPGNPPGSQPKFYDADYAPPGATNVLNHGWDVEDDSG
                     QHIGKVDQVYDNYFSIAEGYLIHNLVYAPFSAIDHVDLQDNAVTLNVSQQQIEALGWD
                     RIPEDVGFGTADNYVVGRTGEIYPNETPEQVERLLRAEGAPGAGRQATPGEQRAAAQR
                     ARQPETRTPGAAEQPETVEVTIVEEVIELGPPEAEPEPPRG"
     gene            complement(2758..3606)
                     /locus_tag="VFU72_02525"
     CDS             complement(2758..3606)
                     /locus_tag="VFU72_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5422391.1"
                     /translation="MADRTDEPGQNGEQEADLVPNQVLNRANRLRRPTEDKLLLAWSQ
                     REREAAQAFIHSDPWRVLRIVSEFVDGFDALAEIGPAVTIFGSARIHAEDPMYAAARA
                     IGRQLVQAGFAVITGGGPGVMEAANQGAAEAGGLSIGCNIELPMEQGTNPYVNLSLDF
                     HYFFVRKTMFLKYAEGFIIFPGGFGTLDELFESLTLIQTGKLETFPVVLFGSAYWQGL
                     LDWMDNFLLKQGKINAEDRGLLRVTDDPEEAVRIMLDCFIEAEMKREAAPHNLATPLD
                     IPNRPT"
     gene            complement(3674..4387)
                     /locus_tag="VFU72_02530"
     CDS             complement(3674..4387)
                     /locus_tag="VFU72_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU5422392.1"
                     /translation="MDGALSERRRQVEAIEPLRRLELRLPRAQRTLDLLLPADLDPLI
                     DAVEHDPEQNLPYWAELWPSGIALADAVLAAPKLVRGRRVLELGCGLGVTACAALYAG
                     AELTVADYAPEALAFCRYHALLNTGREPAALQINWRAPSAELLAAAGDGFDALLAADV
                     LYESRDVQPLLNLLERLLAPGGFLWLAEPGRAVARRFVELSRARGWEGPCETHPGPWP
                     DPRDDEVTVSIYRLRRPGP"
     gene            complement(4389..5750)
                     /locus_tag="VFU72_02535"
     CDS             complement(4389..5750)
                     /locus_tag="VFU72_02535"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5422393.1"
                     /translation="MSAGQEQEIAARAVELARRAGASDAEATVTTGRELAVTVREGEI
                     DYLREAGTRGLHLRVFADQRAALVSGADLSPDGLARLAEQAVALAQLAAPDPSAGLPD
                     GPFGGDDAALELYDPELESLDTPALTALAREADDAARGHDRRIGTTDGATLTRWLGTT
                     TLANSRGFAGSYQESRCALLASALALGDGHERRQEYWQESGRYLAQLGEAEAVGRAAA
                     RRALRQLGAGKVATREVPVVFAPEMARELIRLLARAASGEARYRGFTFLAGREGQALA
                     SPLVTLIDDATLPGRLGSRPFDAEGVASQRTPLLSAGRFQGFLYDSYSARRAGRASTG
                     NAGRQETPIGVRFGVTPSNLVLAAGETAPEAIIAGVEQGLYLTGTSGSGDNLTTGDFT
                     RGASGVWIERGALTHPVAEINVAGRLPEMLAAIDAVGSDLAIEGGAAAPTIRVARMMV
                     SGM"
     gene            complement(5747..>6212)
                     /locus_tag="VFU72_02540"
     CDS             complement(5747..>6212)
                     /locus_tag="VFU72_02540"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5422394.1"
                     /translation="YMQDWQSARALGVPASGNGRRQDFRHAPMPRMTTTYTLAGETPP
                     EEIIRAVDYGVYCVSFSGGQVEIASGDYTFATMEAYLIEHGRITAPIRTVNLIGNGPD
                     SLTRVKLVGNDLRIDEMAGNCGKGGKGEQWVPVGVGLPTVLLDGITVGGTKR"
BASE COUNT          849 a         2304 c         2156 g          903 t
ORIGIN      
        1 caggtccttg gccactgcct ccgccaccga gcgcgcatcg ccggtgatca tgactacgcg
       61 cacgccgcgc cggtgcagct cgtcgatcgc cgcgcgcgac tccggccgca cccggtcctc
      121 cagcgccagc gccccgatca cctgcccgtc gcggatgacg tgcaggatgc cggcgccgcg
      181 cgccgcccag ggccgcatac gcgcctccag ctcgggcggg acggtcaacc cctgctcgcg
      241 cagcatggcc ggcccgccga ccgccacggc ttggccctcc acggtcgcct ccacgccccg
      301 gcccgcgctg gcgcggaagt ccgccgcgcg cggcagcgcc agatcgcgct ccgcggccgc
      361 cgcgacgatg gcgccggcca ccggatgctc gctgtccgac tcggcggcgg cggccagccg
      421 cagcaccgca tcctcgtcgc cggacaccgg cacgaccgcg ctcaacgcgt agctgccgag
      481 cgtcagcgtg ccggtcttgt cgaagaggac ggtgtcgatg gtgcgcatgc gctccagcgc
      541 catgcggtcg cggatcagaa tcccctgccg cgccgccagc gaggtcgaga gcgcgaccac
      601 cagcgggatc gccaggccca gcgcgtgcgg gcaggcgata accagcaccg tgaccgtgcg
      661 tacgacggcg tcgtcgatga cgcccagcag cacccagacg acgaaggtca ccagcccggc
      721 tccggcggcg gcgtagaaga gccagccggc gaaccggtct gccagccgct gcgcgcgcga
      781 ctgtgacgat tgcgcctcgg ccaccagccg ctcgatgccg gccagcgccg tctccgcgcc
      841 gaccgcctcg acgcgcacgc gcaccgacga gccggccacg accgtcccgg ccaccacctt
      901 gtcacccggc cccttgggga tggggcgtga ctccccggtg agcatcgact cgtcgagctc
      961 ggcctgcccg tcgacgatcg cgccgtcggc cggcacccgg ccgccggggc gcaccagcac
     1021 gacgtcgcca acctgaaggt ccgcgaccgg cacgctctgg acctcgccgt cgaccacgcg
     1081 ctccgcctgg tccggcagca gcgccgccag cgcgctcaac gcgccctgcg cctgccccag
     1141 cgctcgcatc tcgagccaat gacccaagag catgatgtcg atcagcagcg ccagttccca
     1201 ccagaagtcg aggccgaaca acccgaaggt ggtggcgagg ctggcgacga acgcgaccgt
     1261 gatcgccatg ctgaccagcg tcatcatgcc cggcttgcgc tcggagagct cctcccgggc
     1321 gccggtcagg aagacccagc cgccatagag gaagacgatg gtgccgagga tgggggcgat
     1381 caggtcgctg ccggggaaag acggcgcgct gtagccgagc cagtcctgca ccatcgggct
     1441 gaagaccacc accggcagcg agagcaccag gctgacccag aagcgccgct tgaagacatc
     1501 ggcatgcccc gcgtgcccgg cgtgcccggc atgcgacgca tgcggcgccc cgctggcatg
     1561 ctcatcgtgt cccgcgtggc ccgcctgcgc tccgtggccg tgcgcctcac gaggcgcgtg
     1621 ctcgatgtgc tcggcgtgcc cggcatgggc gtcgtgcccg tgctggatct gctccgtatg
     1681 cccttcgtgc ccggtgtgcg cgtgctgctg gtgccgcgcc ggcgccggcg cgtccgtgcc
     1741 gcgctcgtcc tgcggtggcg tgtggttgtg catggggtat ttcctcgctg tcgcgcggtc
     1801 taccggcgcc actcgcgccc gtgcgacgct acgcgatctc cgagccgcgt ctcgactcgt
     1861 agcctagtat atacccccca ggggtattgt caagctcaat tcgatcactc gacgacgcgg
     1921 acatgggtgg acggtggggc gtggtttcgt gaaagcgagt gcagcgcggc gcccgggaat
     1981 tcgattcccg ggcgccaggc gccaccgaac ctccacggag tgaggcaaac catgcccgaa
     2041 gcgcgtggtc ccggcctcgg aatcagaagc gagatcagcc gcgcggcggc tccggctcag
     2101 cctccggcgg gcccagctcg atgacctcct cgacgatcgt cacctcgacg gtctcgggct
     2161 gctccgcggc tcccggcgtg cgcgtctccg gctgtcgagc gcgctgcgcg gcagcacgct
     2221 gctcgcccgg cgtagcttgc cggccggctc cgggcgcgcc ctcggcacgc agcagccgtt
     2281 cgacctgctc gggggtctcg ttgggataga tctcgccggt tcgccccacc acgtagttgt
     2341 ccgccgtgcc gaagccgacg tcctcgggga tgcggtccca gccgagcgcc tcgatctgct
     2401 gctggctgac gttgagggtg accgcgttgt cctggagatc gacgtggtcg atcgcgctga
     2461 agggggcgta gaccaggttg tggatcaggt agccctcggc gatgctgaag tagttgtcgt
     2521 agacctgatc gactttaccg atgtgctgtc cgctatcgtc ctccacgtcc cagccgtggt
     2581 tgaggacatt ggtcgcgccc ggcggggcgt agtcggcatc gtagaatttc ggctgactcc
     2641 ccggcggatt gccgggccgg ttatcgcgct cgaagaccat ctccgcgctc ctttcgctgg
     2701 ccgcgcacgc ggcaccgcgc atggcgcacg gctcgtgcca cttagccgcg cggaggctca
     2761 ggtcgggcgg ttcgggatat cgagcggggt ggccaggttg tgcggagcgg cctcgcgctt
     2821 catctccgcc tcgatgaagc agtcgagcat gatccgtacc gcctcctcgg gatcatcggt
     2881 cacgcgcagc agcccgcggt cctcggcgtt gatcttcccc tgcttgagca ggaagttgtc
     2941 catccagtcg agcaagccct gccagtaggc cgagccgaag aggacgaccg gaaacgtttc
     3001 cagcttgccg gtctggatca gggtcagcga ctcgaacagc tcatcgagcg tgccgaagcc
     3061 gccggggaag atgatgaagc cctcggcgta cttgaggaac atcgtcttgc gcacgaagaa
     3121 gtagtggaaa tcgagcgata gattgacgta gggattcgtc ccctgctcca tcggcagctc
     3181 gatgttgcag ccgatcgaga gcccccccgc ctcggcggcg ccctggttgg ccgcctccat
     3241 cacgccgggg ccgccgccgg tgatcacggc gaagcccgcc tgcaccagtt gccggccgat
     3301 ggcgcgcgcg gccgcgtaca tcggatcctc ggcgtggatg cgggccgagc cgaagatggt
     3361 gacggccggg ccgatctcgg ccagcgcgtc gaagccatcc acgaactcgc tgacgatgcg
     3421 cagcacgcgc cagggatcgc tgtggatgaa ggcctgcgcc gcctcgcgct cgcgttgcga
     3481 ccaggccagc agcagcttgt cctcggtggg gcggcgcagc cggttggccc ggttgagcac
     3541 ctggttcggc accaggtcgg cctcttgctc accgttctgc ccgggctcgt cggtccggtc
     3601 cgccatcatc gtctccttcc cgctgcccca ccgccctcac gcacggagcc cccgcccgta
     3661 cgggctgtct atcttacggc ccgggccgcc gcaagcggta gatgctgacc gtgacctcgt
     3721 cgtcgcgcgg atcgggccag gggccgggat gcgtctcgca cggcccctcc cagccgcgcg
     3781 cccgcgacag ctcgacgaag cggcgcgcca ccgcgcgtcc cggctcggcc agccagagga
     3841 atccaccggg cgccagcagc cgctccagca ggttgagaag cggctgcaca tcgcgcgatt
     3901 cgtagagcac gtccgcggcc agcagcgcgt cgaagccgtc gccggccgcg gccagcagct
     3961 ccgccgaggg cgcgcgccag ttgatctgca acgcggccgg ctcgcgcccg gtattgagca
     4021 gcgcgtgata gcggcagaag gccagcgcct cgggcgcgta gtcggccacg gtcagctcgg
     4081 ccccggcgta cagcgcggcg caggcggtga cgcccaggcc gcagccgagc tccagcacgc
     4141 gccgcccgcg caccagcttc ggcgcggcca gcacggcgtc ggccagcgcg atgccgcttg
     4201 gccacagctc ggcccagtag ggcaagttct gctcggggtc gtgctcgacc gcgtcgatca
     4261 gcgggtcgag gtcggccggc aggagcaggt cgagcgtgcg ctgggcgcgc ggcagccgca
     4321 gctcgagccg gcgcagcggc tcgatcgcct cgacctggcg ccgccgctcg ctgagggcac
     4381 cgtccacctc acatcccgct gaccatcatg cgcgccacgc ggatcgtcgg cgccgccgcg
     4441 ccgccctcga tggccagatc gctgccgacg gcgtcgatcg cggccagcat ctccggtagc
     4501 cgcccggcga cgttgatctc ggccaccggg tgggtcagcg cgccgcgctc gatccagacg
     4561 ccgctggcac cgcgcgtgaa gtcgccggtc gtcaggttgt cgccggagcc ggaggtgccg
     4621 gtcaggtaga ggccctgctc aacgccggca atgatcgcct ccggcgcggt ctcgccggcc
     4681 gccagcacca ggttgctcgg cgtgacgccg aatcgcacgc caatcggggt ctcttgccgg
     4741 cctgcattgc cggtgctggc acgccccgcg cggcgcgcgc tgtagctgtc gtagaggaag
     4801 ccctggaagc gcccggccga cagcagcggt gtgcgctggg aagcgactcc ttcggcgtcg
     4861 aaggggcgcg agccgagccg gccggggaga gtggcgtcgt cgatcagtgt caccagcggc
     4921 gaggccagcg cctggccctc gcgcccggcc aggaaggtga agccccggta gcgggcctcg
     4981 cccgaggccg cccgggccag gagccggatc aattcgcgcg ccatctcggg cgcgaagacg
     5041 accggcacct cccgcgtcgc caccttgccc gccccgagct gccgcagcgc ccgccgcgcc
     5101 gccgcgcgcc caaccgcctc ggcctcaccg agctgtgcca gatagcgtcc gctctcctgc
     5161 cagtactcct ggcgccgctc atgcccgtcg cccagcgcca gcgccgaggc caacaacgca
     5221 cagcggctct cctggtagct tccggcgaag ccgcggctat tggccagcgt ggtggtcccc
     5281 agccagcgcg tgagggtggc accgtcggtg gtgccgatgc gccggtcgtg gccgcgcgcg
     5341 gcgtcgtcgg cctcgcgcgc gagcgcggtc agcgccgggg tgtcgagcga ctccagctcg
     5401 ggatcgtaga gctcgagggc ggcgtcatcg ccgccgaagg gaccgtccgg caggccggca
     5461 gaggggtcgg gcgcggcgag ctgcgcgagc gcgactgcct gctcagccag gcgtgccagt
     5521 ccgtccgggc tgaggtcggc gccgctgacc agcgccgcgc gctggtccgc aaagacgcgc
     5581 aggtgcaggc cgcgcgtgcc ggcttcgcgc agatagtcga tctcgccctc gcgcacggtc
     5641 accgccagct cgcgcccggt ggtcacggtg gcctcggcgt cgctggctcc agcacgccgc
     5701 gccagctcga ccgcgcgcgc cgcgatctcc tgctcctgcc cggcgctcat cgcttcgttc
     5761 ctcccacggt gatgccgtcc agcagcacgg tcggcagtcc caccccgacc ggcacccact
     5821 gctcgccctt gccgcccttg ccgcagttcc cagccatctc gtcgatgcgc agatcgttgc
     5881 cgaccagctt gacgcgggtc aggctgtccg gtccgttgcc gatcaggttg acggtgcgga
     5941 tcggtgcggt gatgcggccg tgttcgatca ggtaggcctc catcgtggcg aaggtgtagt
     6001 cgccgctggc gatttcgacc tgcccgccgc tgaagctgac gcagtagacg ccgtagtcga
     6061 ccgcgcggat gatctcctcg ggcggagtct cgccggccag ggtgtaggtg gtggtcatgc
     6121 gcggcatcgg cgcgtggcgg aagtcctgcc gccgcccatt gcccgaggcc ggcacgccga
     6181 gagcgcgcgc cgactgccag tcctgcatgt ag
//
LOCUS       DASUBI010000133         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_704883, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000133 DASUBI010000000
VERSION     DASUBI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704883"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFU72_02545"
     CDS             <1..577
                     /locus_tag="VFU72_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5422395.1"
                     /translation="GRLVQSLLTLARADVGWRPALETLALDQVAEDVARVAAPLAAQH
                     QFSVNVHDEVDVLGNADQLKQLLLILLDNAFAYTPPGSEVSLTVRQVGDTAEMIVIDH
                     GPGIPPDELDHIFDRFYRGDVARARRAAGAGLGLAIARWIVDCHHAEVSVQSTEHQGT
                     IITVRLPAVTSAEPDGPRELEPLAAVGSTTN"
     gene            complement(628..2559)
                     /locus_tag="VFU72_02550"
     CDS             complement(628..2559)
                     /locus_tag="VFU72_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5422396.1"
                     /translation="MRELIDRQERFALGVCYYPEHWPRDRWERYARQMREVGLSYVRI
                     GEFAWSRIEPREGQFAWSWLDEAVETLAAAGLKVVLGTPTAAPPVWLARKHPEILSVD
                     AGGRRRDHGGRRHYDFSSRVYREHARRITRVIAERYGTHPTVVGWQTDNELSEHQSYS
                     SEALHAFREWLGERYAGIGQLNTAWGTVFWSQEYGDFDEITFPNLVVEAPNPSQQLDF
                     YRFCSDQLARFQEEQVAILRALSPGRWVTHNFMRLQPDFDHYRAAAGLDFVAWDNYPT
                     GGVELARLSPEEQRTWARTGHPDLIALNHDLYRGLLGGRSFWVMEQQCGQINWGRTNP
                     LPAPGAVALWTAQAWGHGANVVSYFRWRAATMAQELMHSGLLRHDESLDRGGAEVAAL
                     DLGKLPNVPVTTSVVLLHDYESLWAYDLQPHNQAASYWDQFLLFYVALRSLGVDVDIR
                     HPDADLSPYRLIVAPALQLMPDSRARRLASYAGSAELLFGPRTAYRTPSGRVHEGGQP
                     GPLRDLLGCSLLNVDGIPPGASVAIGRHTAITWAESYRPTTGTATQVYTGGPLAGGAA
                     AVRHGSVTTIGAWSPTLISEALEATLGRAGISTLRLPNGVRVSRRGRFEVWVNFSQRQ
                     AVASGDLRLEPVSYRIRER"
     gene            complement(3093..3524)
                     /locus_tag="VFU72_02555"
     CDS             complement(3093..3524)
                     /locus_tag="VFU72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEU5422397.1"
                     /translation="MRRSPAPLPLPGASQSHSQVSLMSDCSTCPTLALTVVAHRLRTN
                     EAAVQRNRRNAMARLSQEEENKLPSSDFAFPKERKEPLVDASHVRDAIARFDQVEGVS
                     NHERDEAWKRIVAAAKKYDIQMSESNWRELFTRNGRAVPKD"
     gene            3506..>3861
                     /locus_tag="VFU72_02560"
     CDS             3506..>3861
                     /locus_tag="VFU72_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5422398.1"
                     /translation="MPEIDASPRAATAPLIHDSVLEVIGSTPLVRLSRLTRGLKATVA
                     AKLEMLNPGGSVKDRIGFRMIDEAERRGWLRPGGTIVEPTSGNTGVGLAMAAAIRGYR
                     CVFVMPDKVSAEKVALL"
BASE COUNT          623 a         1309 c         1319 g          610 t
ORIGIN      
        1 gggccggctg gtgcagagcc tactgacgct ggcccgggct gatgtgggct ggcggccggc
       61 gctggagacg ctggcgctcg atcaggtggc agaggatgtg gcgcgcgtgg cggcgccgct
      121 ggctgcccag caccaattct cggtcaacgt ccacgacgag gtcgatgtcc tgggcaacgc
      181 cgaccagctc aagcaactgc tgctgatcct gctcgacaac gccttcgcct atacgcctcc
      241 gggaagcgag gtctcgctta cggtgcggca ggtcggcgac acggccgaga tgatcgtgat
      301 cgatcatgga ccgggtattc cacccgacga actcgatcac atcttcgacc gtttctatcg
      361 cggcgacgtg gcgcgagcgc ggcgcgcggc cggtgcagga ctggggttgg ccatcgcccg
      421 ctggatcgtg gattgccacc acgccgaggt ctcggtgcag agcaccgagc accagggcac
      481 gatcatcacg gtgcgcttgc cggcggtgac gagcgcggag ccggacggcc cgcgcgagct
      541 tgagccgttg gcggcggtcg gctccacgac caattgatgg tccggttcaa gccctgtcgt
      601 gttgccgcct ctcccagtcc ttgaccgtca ccgttcccgg atacggtacg acacgggttc
      661 aaggcggaga tcgccgctcg ctaccgcctg ccgttgcgag aagttcaccc acacctcgaa
      721 cctcccgcga cgcgagacgc gcacgccgtt gggcagtcgc agggttgaaa tccccgcgcg
      781 cccaagcgtg gcctccagag cctcggaaat cagcgtggga ctccaggcgc cgatcgtcgt
      841 cacgctcccg tggcgcacgg ccgcggcgcc gccggccagc ggcccgccgg tatacacctg
      901 cgtggccgtg cctgttgtcg gccggtagct ctcggcccag gtgatggcgg tgtgccggcc
      961 gatggcgacg gaagcgccag ggggaatgcc gtcgacgttc agcagcgagc agccgagcag
     1021 gtcgcgcagc gggcccggct gtccgccctc gtgcacccgg ccgctgggcg tgcgataggc
     1081 ggtgcgtggc ccgaacagca gctcggccga accggcgtag gacgccaggc gcctggcgcg
     1141 cgaatccggc atgagctgca gcgcgggggc gacgatcagc cggtaggggg agaggtctgc
     1201 gtcggggtga cggatgtcga catcgacgcc gagagagcgc agcgcgacat aaaacagaag
     1261 aaactgatcc cagtaggacg ccgcctggtt gtgcggttgc aggtcgtacg cccagaggct
     1321 ttcgtagtcg tgcagcagta cgaccgaggt cgtcaccggc acgttcggca gcttgccgag
     1381 gtcgagcgcc gccacctccg cgccgccccg gtcgagcgac tcgtcgtgcc gcagcaagcc
     1441 cgagtgcatc agctcctgcg ccatggtggc ggcgcgccag cgaaagtagc tcacgacgtt
     1501 cgcgccgtgg ccccaggcct gcgccgtcca gagcgcgacc gcgcccggcg ccgggagcgg
     1561 gttcgtgcgc ccccagttga tctgcccgca ctgttgctcc ataacccaga acgaccgccc
     1621 gccgagcagg ccgcgataga ggtcgtggtt gagcgcgatc aggtcggggt ggccggtgcg
     1681 cgcccaggtg cgctgctcct ccggcgacag cctcgcgagc tcgacgccac cagtcggata
     1741 gttgtcccag gcgacgaagt cgaggccggc ggccgctcgg tagtggtcga agtccggctg
     1801 cagccgcatg aagttgtgcg tcacccagcg gcccggcgac aaggcgcgca ggatggcgac
     1861 ctgctcctcc tggaagcggg cgagctgatc ggagcagaag cggtagaagt cgagctgctg
     1921 cgacggattc ggcgcctcga cgacgagatt ggggaaggtg atctcatcga agtcgccgta
     1981 ctcctggctc cagaagaccg taccccaggc cgtgttgagc tggccgatgc cggcgtagcg
     2041 ctcgcccagc cactcgcgga acgcgtgcag cgcctccgat gagtacgatt gatgctcgga
     2101 cagctcgttg tcggtctgcc agccgacgac ggtcgggtgc gtcccgtagc gctcggcgat
     2161 gacgcgggtg atccgccggg cgtgctcgcg gtagacccgg ctggagaaat cgtagtggcg
     2221 ccgcccgccg tgatcgcgcc gccggccgcc ggcatccacg ctcaagatct ccggatgctt
     2281 gcgcgccaac cagaccggcg gcgccgcggt tggcgtgccg aggacgacct tcagtcccgc
     2341 ggccgccagc gtctcgaccg cctcatccag ccagctccag gcgaactggc cctcacgagg
     2401 ctcgatccgg ctccaggcga attcaccgat gcgcacgtag gacagcccga cctcgcgcat
     2461 ctgccgggcg taccgctccc agcgatccct cggccagtgt tccgggtagt aacacacccc
     2521 gagcgcgaag cgctcttgcc tgtcgatgag ctctcgcatc ccatgcctca atcacctgga
     2581 caacagggta cggctccagc gatgggatag ccagggcaga ccggacctgc gcgacgacga
     2641 gcagcgatgc actcgccgtc atgagcggca gttgttcgag agatcgtcgc ccaccgctcg
     2701 cgtcgatggg cgccatgggg agcgtcggcg agatgcgtcg actacggccc cggcacgacc
     2761 ggcaggacga cagccccacc ccgctgtcag cctgagtcgc ggcgaacggt ctcctctggg
     2821 gtgcagcgcc gcgctcgctc cacgatcaag agagatcctt cggccgcggc ctcaggctga
     2881 caggagtggg aaagggcctc tcctcatggt ggaaaaggtg caatcacgta caccagggga
     2941 gatcccttcc ttcggcaagc tcaggaccag gctcagggca ggctcttcga cagcggccgc
     3001 gggatgaccg ggaaggcaag gtcctcccca ggacttcatc gggcgccggc ggcgacccca
     3061 cccgcgctcc attcgattcc gtgaacctcg cgctagtcct tcggcaccgc gcgtccgttg
     3121 cgcgtaaaga gctcgcgcca gttcgactcg ctcatctgga tgtcgtactt cttggccgcg
     3181 gccacgattc gtttccaggc ctcgtcgcgc tcgtggttgc tcacgccctc gacctggtcg
     3241 aaacgcgcga tcgcgtcgcg cacgtggctc gcgtcgacca gtggctcctt gcgctccttg
     3301 ggaaaggcaa aatcgctgct ggggagcttg ttttcctctt cctggctcag tctcgccatg
     3361 gcgttcctcc tgttcctctg aacggctgct tcgttcgtac gcagacggtg agccaccacg
     3421 gtcagtgcca gagtggggca cgtgctacaa tcgctcatca acgacacctg agagtgcgat
     3481 tggctcgcgc caggaagagg tagaggtgcc ggagatcgac gcatcgccgc gggcagcgac
     3541 cgcaccgctc atccacgaca gcgtcctgga ggttatcggc agtacgccac tggtgcgctt
     3601 gagccggctg acgcgcggtc tgaaggccac cgtcgcggcc aaactggaga tgctcaatcc
     3661 cggcgggagc gtcaaggacc gcatcggctt ccgcatgatc gacgaggccg aacggcgcgg
     3721 ctggctgcgg cccggcggca ccatcgtcga gccgaccagc ggcaacaccg gggtcggact
     3781 ggccatggcc gccgccattc ggggctaccg ctgcgtcttc gtgatgccgg acaaggtctc
     3841 ggctgagaag gtcgcactgc t
//
LOCUS       DASUBI010000134         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_732462, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000134 DASUBI010000000
VERSION     DASUBI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732462"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFU72_02565"
     CDS             <1..761
                     /locus_tag="VFU72_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5422399.1"
                     /translation="APEKMVEGRASFVEIPVYQGDTFETITPMRRQIAENMVRSERTA
                     PHVWTWMEVDMSRIVAARTRDKERFAAEEGFSLTYLPFVLKAVVNALREHPEVNAAWD
                     EANNRIIRRKAINLGVAVSLESGLVVPVIKHADERSIVGLARTANELATRARENKLMV
                     DDVQGGTFTVNNPGTFGTVLSTPLIVQPQAAILSTEAVIKRPVVLDDAIAIRPIMNLS
                     LSIDHRILDGLAAARFLSAVKGWLERFDPESSLY"
     gene            1358..>2406
                     /locus_tag="VFU72_02570"
     CDS             1358..>2406
                     /locus_tag="VFU72_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422400.1"
                     /translation="MGRLQLGVFVLGVALVVAACTISGPREPDSSQTATLTGVAPSPT
                     IPLAINSSTPPTPTPKPTASPTPSPTPSPTPSPTPTPTPPPPPLARCYQPRQVEAAAP
                     VATPAAPLGTPQTASEEAATPAPPPGPVLRPASPTSVFRDCQVVAYYGFPGNDQMGIL
                     GTADPATIVKKLKEQAAAYDAVNGSRGVAPALELIYAVAQGSQTSDGTYLSRMSDDMV
                     KEYLDIAEKNDLLLILDIQMGSSNIQKEWPHVLPYLKNPRVHLALDPEFVVPDPDTPP
                     GRVIGNMDVSQINQAEELLQKLVEEERLPNKILVVHQFIPDMILNKDKMQTQPNVDLV
                     IDQDGFGSAELKSSN"
BASE COUNT          434 a          838 c          763 g          371 t
ORIGIN      
        1 tcgcgccgga gaagatggtc gagggccgcg ccagcttcgt cgagatcccg gtctatcaag
       61 gtgacacgtt cgagacgatc acgcccatgc ggcggcagat cgccgagaac atggtgcgca
      121 gcgagcgcac cgcgccgcac gtgtggacct ggatggaggt cgacatgtcg cggatcgtgg
      181 cggcgcgcac ccgcgacaag gagcgcttcg ccgcggagga gggcttcagt ctgacctacc
      241 tccccttcgt gcttaaggcg gtggtcaacg ccctgcgcga gcaccccgag gtcaacgccg
      301 cctgggacga ggccaacaac cgcatcatcc ggcgcaaggc gatcaacctc ggggtggcgg
      361 tcagcctgga gagcgggctg gtggtgccgg tcatcaagca cgccgacgag cgcagcatcg
      421 tcgggctggc gcggacggcg aacgagctgg ccacgcgcgc ccgcgagaac aagctgatgg
      481 tcgatgacgt gcagggtggc accttcacgg tcaacaaccc cggcaccttc ggcaccgtcc
      541 tctccacgcc gctgatcgtg cagccacagg cggccatcct cagcacggag gcggtcatca
      601 agcgcccggt ggtgctcgac gacgccatcg ccatccggcc gatcatgaac ctgtcgctct
      661 cgatcgacca tcgcatcctc gacggcctgg ccgctgcccg cttcctgagc gcggtcaagg
      721 gctggttaga gcgcttcgac cccgagagct cgctgtatta atcgcgagcc tcgtcttgtc
      781 accggagcct cgccaggggc tccggtgaac gctgtcttgg aggtttcgcg cctcacgcag
      841 cgcccgggat ccccgaacca tcgaagagat acttcggctg cgacctcagc gcctgacctg
      901 ggcctgtcga agggatgaca aggaagagag agtcgctatc tttgtcatcc cgagccgcag
      961 caaagaatct ctcatcagca acaagcgact cctagagcgt cgggcacgta ccggtctggg
     1021 acggcgagac gccgttagcc ggacggtcgt tgccacccca ggctgagggg agatccttcg
     1081 cggcggttca ggatgacagg gggagggctg tcttttctct gtcatcctga ggccgcagcc
     1141 gaaggatctc ttcggtgttg cccagttgcc cggcgttacg tgaccggtgc tcgtgcctcc
     1201 gaacggcggg cccaactcgg cgtcacatgg cctcgctctt gtccccagca gcctctcatg
     1261 gggtaggatt gccaactcgt agcggaatgc gccttgctcg tgcgcgaggc caggtgcggc
     1321 gcggatacag gagctgggcg cgagagggga ttttcgggtg ggccgactgc agttgggggt
     1381 cttcgtgctg ggggtggcgc tggtggtggc cgcctgcacc atctccggac cccgcgagcc
     1441 ggactcttcc cagaccgcca cgctgaccgg cgtcgcgcct agcccgacga taccgctggc
     1501 gatcaactcc agtacccccc cgacaccgac gccgaaaccg accgccagcc cgactccttc
     1561 acccaccccg tcgccgacac cctcaccgac accgacgcct actccgcccc cgccgccgct
     1621 ggcgcgctgc taccagccgc gccaggtcga ggcagccgcg ccggtcgcga cgccggccgc
     1681 gcccctcggc acaccccaaa cagcgtcgga ggaagcagcg actcccgctc cgccgcccgg
     1741 cccggtgctg cgcccggcca gcccgacctc ggtcttccgc gactgccagg tggtcgccta
     1801 ctacggcttc cccggtaacg accagatggg catcctgggc accgccgacc ccgccacgat
     1861 cgtcaagaag ctcaaggagc aggccgccgc ctacgacgcg gtcaacggct cgcgcggggt
     1921 ggcgccggcg ctggagctga tctacgccgt ggcccagggt agccagacct ccgacggcac
     1981 ctacctctcg cgcatgtcgg acgacatggt caaggagtat ctggacatcg ccgaaaagaa
     2041 cgacctgctg ctcatcctcg acattcagat gggcagcagc aatatccaga aggagtggcc
     2101 gcacgtcctc ccctacctga aaaacccgcg cgtccacctg gcgctcgatc ccgagttcgt
     2161 ggtgcccgat ccggatacgc cgccgggccg cgtgatcggg aacatggacg tctcgcagat
     2221 caaccaggca gaggagctac tgcagaagct ggtcgaagag gagcgcctgc cgaacaagat
     2281 cctggtcgtg catcaattca tccccgacat gatcttgaac aaggacaaga tgcagacgca
     2341 accgaacgtc gatctggtca tcgaccagga cggcttcggc agcgccgagc tgaaaagctc
     2401 caacta
//
LOCUS       DASUBI010000135         9037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_677752, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000135 DASUBI010000000
VERSION     DASUBI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677752"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1484
                     /locus_tag="VFU72_02575"
     CDS             459..1484
                     /locus_tag="VFU72_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5422401.1"
                     /translation="MARTGAAPATALARRLNRRAVVGLMAVPLLSLLEPNVSEARLGG
                     STAQGAVYLGVWQPGAPQNLTVLDRFERDAGRDVAIVHWYQGWGAANAALDPALLASV
                     RARAAAPMITWEPWDYTKGVAQPSYSLARIADGDFDGYIRGWSTGLAAYGGPVLLRFA
                     HEMNNPVYPWSIGVNGNTADDYLRAWRHVHDIFTASGASNVQWVWSPLIWWPGAPELE
                     PLFPGDDVVDWVAVDGYNNPDWGGWQSFNQLFGPSYQKLTSLSSRPVMIAEIGCAEQG
                     GSKADWITDAFRTGIPSMPRLRAVVWFNEDQRASIGVDWRIESSGAARRAYARAISSS
                     WYRSAWP"
     gene            complement(1470..4310)
                     /locus_tag="VFU72_02580"
     CDS             complement(1470..4310)
                     /locus_tag="VFU72_02580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422402.1"
                     /translation="MSLRLQHEEVRDAPLGAEQPVVAFRRVLSRRQRVLLAVVIAADL
                     LVAIGFVVWLALPSHLPLAADSRGPLVTVLGLAGFGALLVLELTRAAQSATLWLFAWK
                     MRDPVPMLPEPGLRVAVLTTIVPSKEPLALVEETLAAMRNIRYHGQVDVWILDEGNDP
                     AVRRMAAKLGVKHFSLQGRPEYNQPSGPFRARTKAGNHNAWRAEHERDYDIVAQMDPD
                     HVPFPSLLARTLGYFRDPDVAFVVAPQVYGNRDDSFIVGAADDQSFVFHGVVQRGGNG
                     LGAPLLIGTNHLYRTSAWRQIDGYQDSVIEDHLTSLTVNGSENPETGNRWKGIYTPEI
                     VAVGEGPRSWTDYFNQQQRWAYGIWEVILKHAWRKLWRLSWSQRLSYLALELFYPSVA
                     VIWVLGSLLTALYLILGVTVIDLPLRTWSIFWLATFFTQYGTFLWLRRFNLQAHERAG
                     SGLNGMLLSIAAGPVYVAAAAAALCRRPLSYVVTAKSDLASADSLATFRSHFSWAVFA
                     AVMLVASLLMGHNHWPLRAWALVTLAAVAAPVAAHLSGRLTRRAARPRLALASAALAA
                     VLVVPMVAGATVLWGNRLLDGAAEQEAAVIGIQPPAGNVGQHPAAPAAGQGHSGEQGS
                     AVIARPTPPKLLVPGRGDAPVMFGIFDQQTGPQTGDIAAVYYQWTAQPENFARFVQGQ
                     AQAGRTALITWEPRSGEIQATPTAHDTDLLLEIASGRQDAYITSVADALAATKQPVII
                     RFAEEMDLATSGLHPWAGHPPAIYVAAYQRMVTLFRAAGADNVLWLWTPSAMTDAQGN
                     LTSMPYYPGDAYVDYTGFDAFLYWRWEEWNPQRQAARAYRSPDEYLTRPIQQLAALGK
                     PVIIPELGVDLGSDRSAAKQRWIEQAGNLMAQGHYPGLAAVVYFDAQHNWDDRQADWR
                     LSPDDSQSFLVPFQRAASGMPAGPTGPPLRPR"
     gene            complement(4994..6133)
                     /locus_tag="VFU72_02585"
     CDS             complement(4994..6133)
                     /locus_tag="VFU72_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5422403.1"
                     /translation="MPAVTIEDVRQRESELLDSLRAIVELESPSNDKTAVDRLARYLQ
                     ERCADLGGAVEVLPQAAYGDLTLANWKAEQGAAQPVLVLTHIDTVWPLGTLARKPFTV
                     EGRVARGPGVFDMKASVAMMLEALRLIRDQHLPHRAIQWLINTEEEVGSPVSRPFIED
                     LARQADYVLVLEPPVPPHGALKTARKGVGMFTMEIRGRAAHAGADHEAGISAIQELAN
                     QIQYLHGLTDYGLGTTVNVGVVSGGSARNVVAAEARAEIDVRVTTAREAERITRSILQ
                     AQPRLPGTKLSISGGLNRPPMERTERIAAAFGRAREIGASLGLDLGEASTGGGSDGNF
                     TAAVGAATIDGLGCPGEGAHAEHEHILLDQLVERTALLTALLTSL"
     gene            complement(6147..7151)
                     /locus_tag="VFU72_02590"
     CDS             complement(6147..7151)
                     /locus_tag="VFU72_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422404.1"
                     /translation="MKTYRTIQYGLGPIGQEVARLALAHPGIEVAGAVDIDPNKVGKD
                     LGQVVGLGRDVGVTVSSDAGQVFQAANADVVLHTTGSSLRQVMPQLDGILKAGLNVVS
                     TTEELAYPVANRVELAQQLDQLGKARGATLLGTGVNPGFVMDSLPIYFTAIAQKVDKV
                     FIERLQNASTRRQPLQAKIGSGKTIEEFRQLVEAGKVRHVGLQESVFMLAAALGWQLD
                     DYSESTNPVVADHQITTQYFDVKPGQVAGVDQLGLGFVKGQEKIRLHLKMYLDANPAQ
                     DRVMLEGPTPLELIIPGSLQGDRATTAIVMNSIPQVVAHSGGLVSMIDLPLVHAWGQQ
                     "
     gene            complement(7195..7533)
                     /locus_tag="VFU72_02595"
     CDS             complement(7195..7533)
                     /locus_tag="VFU72_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422405.1"
                     /translation="MYLADALDRIRERLQTNNARVETMRLLDTIGRQPGADRAQVGSQ
                     LELVRRLMRTPVANANVGVYDDLAVLEEQMASASAEASARRAAEESKPMPKSKKYYKT
                     LREREEKKSS"
     gene            7749..>9037
                     /gene="ribA"
                     /locus_tag="VFU72_02600"
     CDS             7749..>9037
                     /gene="ribA"
                     /locus_tag="VFU72_02600"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU5422406.1"
                     /translation="MTLRSSPPDDPETGALRRLVSTRLPTAEGSFRLCLYDSDDGKEH
                     LALVLGEVAGQAGVLVRVHSECFTGDVLGSLRCDCGEQLRRSLALIAAEGRGVLLYLR
                     QEGRGIGLLDKLRAYNLQDQGFDTVDANLLLGHQPDERDYRVAGEMLDELDVRSIRLL
                     TNNPAKLAALRALGVRVAGRVPLHTAAGPENLAYLTTKARRMAHQLEPEPEEPRGGPG
                     APPRNGHRQRPAVTLAYAQSLDGSLAVRSGEPLALSGPESLVMTHRLRAAHDAILVGI
                     GTVLADDPRLTVRLAPGRHPRPVILDSRLRFPLEARLLAGPRRPWIATGTDSSPERQE
                     ELEAAGARVLRLPLDDAGRIALPALLRVLAARGIERLMVEGGPRVIASFLRQRLADQL
                     VITLAPRLVGGLRLLDQPGDYPSLREPRQARVGQDLVL"
BASE COUNT         1354 a         3053 c         3200 g         1430 t
ORIGIN      
        1 gagaaggtgc ggaacttcct gatgggcgcc gcgacccaga tccaggacgt cgtggcggac
       61 ttccagtcga ccaacggcga gctgccgcag accggcgcgc cgcagcacca gcacccggcg
      121 atgtagccct catcccgcac ggttccaccc gtgcccacct ctcctctctg catcgagggc
      181 acgggagcac cgaaggcgat tctcacagcc aaccccttct ctcatctgcg agagaagggg
      241 tttcgcgcgc ccggtcctag acctcgacct aatttgtagc ctctcccttc ccgaggatgg
      301 ggaccaatga gggggttccc tctggctccg attcgtgtca tcctctgtcc cagacatcca
      361 gccgagcgat tcgctgacaa gtagccgcac ctggcggcgg gaaacaggtg tcctggggga
      421 cactggacac ctgctgggaa gggaagcttg tgtcaccatt ggcacggacg ggcgccgcgc
      481 cggccacggc tctggcgcgc cgtctcaacc ggagggccgt tgtcggactc atggcggtgc
      541 cgctgctctc gctgctggag ccgaacgtaa gcgaggcgcg cctcggcggc agtactgcgc
      601 aaggagccgt ctacctgggc gtctggcaac cgggtgcgcc ccagaacctg acggtactcg
      661 accgcttcga gcgcgatgcc gggcgcgacg tggccatcgt ccactggtat cagggttggg
      721 gcgcggccaa cgccgcgctc gacccggcgc tgctggcgtc ggtgcgggcg cgcgccgccg
      781 cgccgatgat cacctgggag ccctgggact ataccaaggg agtggcgcaa ccctcctaca
      841 gcctggcgcg tatcgcggac ggcgacttcg atggctacat ccgtggttgg tccaccggac
      901 tggcggccta cggcgggccg gtgctgctgc gcttcgccca tgagatgaat aacccggtct
      961 atccctggtc gatcggcgtc aacggcaaca ccgccgacga ctacctgcgt gcctggcgcc
     1021 acgtgcacga catcttcacg gcgagtggcg cctcgaacgt gcagtgggtc tggtcgccac
     1081 tgatctggtg gccgggcgcg cccgagctgg agccgctctt ccccggcgac gacgtggtcg
     1141 actgggttgc ggtcgacggc tacaacaatc cggactgggg cggttggcag agcttcaacc
     1201 agctcttcgg tccgtcatac cagaagctga cgtcgctcag cagccggccg gtgatgatcg
     1261 cggagatcgg ctgcgccgag cagggtggca gcaaggccga ctggatcacg gacgcgttcc
     1321 ggacaggcat cccgtcgatg ccgcgcctgc gggccgtcgt ctggttcaac gaggaccaac
     1381 gcgccagcat cggcgtcgac tggcgcatcg agtcctccgg cgcggcgcga cgggcctacg
     1441 cccgggcgat ctcatcgtcc tggtaccgct cagcgtggcc gtagtggcgg tccggtaggc
     1501 ccggccggca tcccgctcgc ggcccgctgg aacggcacga ggaaggactg gctgtcgtcc
     1561 ggcgagagac gccaatccgc ctggcggtca tcccagttgt gctgggcgtc gaaatagaca
     1621 accgccgcca agccggggta gtggccctga gccatcaggt tgccggcctg ttcgatccaa
     1681 cgctgcttgg ccgcgctgcg atcggagccg agatcgacgc ccagttccgg gatgatcacc
     1741 ggcttgccga gcgcggccaa ctgctggatc gggcgcgtca ggtattcgtc gggtgagcgg
     1801 taggcgcgcg ccgcctgccg ctgggggttc cattcctccc agcgccagta gaggaaggcg
     1861 tcgaagccgg tgtagtcgac gtaggcatcg cctgggtagt agggcatcga cgtgaggttc
     1921 ccctgcgcgt cggtcatggc cgacggcgtc cagagccaga gcacgttgtc cgcgcccgcg
     1981 gcgcggaaga gcgtaaccat gcgctgatag gcggccacgt agatggcggg cgggtggccc
     2041 gcccagggat gcaatcccga cgtggccaga tccatctcct cggcgaagcg gatgatcacc
     2101 ggctgcttgg tcgccgccag cgcgtccgcg acgctggtga tgtaggcgtc ctggcggccg
     2161 ctggcgatct ccagcaggag gtcggtgtcg tgggcggtgg gagtcgcctg aatctccccg
     2221 ctccgcggtt cccaggtgat caatgcggtg cgccccgcct gcgcctgccc ctgcacgaag
     2281 cgcgcgaagt tctccggctg cgcggtccac tgatagtaga cggcggcgat gtcgccggtc
     2341 tgagggccgg tctgctggtc gaagatgccg aacatgaccg gcgcgtcgcc gcggcccggc
     2401 acgagcagct tcggcggcgt tggcctggcg atgaccgcgc tgccctgctc gccggagtgg
     2461 ccttgacccg cggccggggc tgccggatgc tgcccgacat tgccagccgg cggctggatg
     2521 ccgatcaccg ccgcctcttg ctcggctgcg ccgtcgagca ggcggttgcc ccagagcacg
     2581 gtcgcgccgg cgaccatcgg cacgaccagc accgcggcca gtgccgccga cgccagcgcc
     2641 aggcgcggac gggcggcacg gcgtgtcagc cgcccggaga ggtgtgccgc caccggagcg
     2701 gccacggccg ccagcgtgac cagcgcccag gcgcggagcg gccagtggtt gtggcccatg
     2761 agcagactgg ccacgagcat caccgcggcg aacacggccc agctgaaatg gctgcggaag
     2821 gtcgccagcg agtcggcgct ggccagatcg gacttggcgg tcaccacgta gctgagcggg
     2881 cggcgacaga gcgccgcggc cgcggcggcg acgtagaccg ggccggccgc gatcgagagg
     2941 agcatgccgt tgagtcccga gccggcgcgc tcgtgggctt gcaggttgaa gcgccgcagc
     3001 cagaggaagg tgccgtactg ggtgaagaag gtggccagcc agaagatgct ccaggtacgc
     3061 agcggcagat cgatcaccgt caccccgagg atcaggtaga gcgcggtcag caggctgccg
     3121 agcacccaga tgaccgcgac gctggggtag aagagctcga gggccagata gctcaggcgc
     3181 tgcgaccagc tcagccgcca cagcttgcgc caggcgtgct tgaggatcac ctcccagatg
     3241 ccgtaggccc agcgctgctg ctggttgaag tagtcggtcc aactgcgcgg cccttcgccg
     3301 accgccacga tctccggcgt gtagatcccc ttccagcggt tcccggtctc gggattctcg
     3361 ctgccattga ccgtcaagct ggtcaggtgg tcctcgatga cgctgtcctg atagccgtcg
     3421 atctggcgcc aggcgctggt gcggtagagg tggttggtgc cgatcaggag cggcgcaccg
     3481 agaccgttgc cgccgcgctg gaccacccca tgaaacacga agctctggtc gtcggcggcc
     3541 ccgacgatga aggagtcgtc ccggttgccg tagacctgcg gcgcgaccac gaaggcgaca
     3601 tccggatcgc gaaaatagcc cagcgtgcgg gccagcaggc tcggaaaggg cacgtggtcg
     3661 ggatccatct gcgccacgat gtcgtagtca cgctcgtgct cggcgcgcca ggcgttgtgg
     3721 ttgcccgcct tggttctggc acgaaaggga ccggaaggct ggttatactc ggggcgcccc
     3781 tgtagcgaga agtgcttgac gccgagcttg gcggccatgc gccggaccgc gggatcgttg
     3841 ccctcgtcga gaatccagac gtcgacctga ccgtggtagc ggatgttccg catcgccgct
     3901 agcgtctcct cgaccagcgc gagcggttcc ttggagggca cgatggtggt gagcacggcc
     3961 acccgcaggc caggctcggg cagcatcggg accgggtcgc gcatcttcca ggcgaagagc
     4021 cagagggtgg ccgactgcgc cgcgcgcgtc agctccagga cgagcaaggc gccgaagccg
     4081 gcgaggccca gcaccgtgac gagcggaccg cgcgagtcgg cggcgagggg caggtgactg
     4141 gggagcgcca gccagaccac gaagccgatc gcgaccagga gatcggcggc gatgacgacc
     4201 gcgagcagca cgcgctggcg gcggctcaag acgcgacgaa aggccacgac cggctgctcc
     4261 gcgccgagcg gcgcgtctcg cacttcctca tgttggagac gtagcgacat tggcactcct
     4321 gctcttgcgt ccaaacaacc acgtggggtg gacgcaagta ctgtgcctgc caggaaccta
     4381 tcgcttttca gtaactacat tgggccgtgc gttggtcgaa cgcgcgagat ggggcgcggg
     4441 atttagcaat ctcgctgaac cggtggaggt gtcgcaagga gaggacccca caactcccga
     4501 atctgggagc ggagggtagg cgccgcgcgg agcgcgcgcc gaggggctgc ctgacgaaca
     4561 cgttgggtgc cgtcgcggac ggatccttcg actgcgctca ggatgacatg aatgggggat
     4621 gggggctgac gacgacgaac cgcgtcaggc gaggccgcac gtcgtcgtac gccccatccc
     4681 ctccccctgt catcctgagc gcagtcgaag gatccgtcct cccgcggctc ccatgcgcct
     4741 cgacggcagc cggcccctgc gcccaaccag gccagggaag agcccggcgc acggcagccg
     4801 tctgattcct cagctagcgc caggagcgcc gctcgaataa tggtaaagga cggcgaggca
     4861 ccattggctt gacagtcgct gttacccgag gccgaggaga gatccttcac tgcggctgag
     4921 gacgacagga gcgggcgcag ccgaaggatc tcttgggtgt cgcccaattg ccgggcgttc
     4981 cttgaccggc gctctagagc gacgtcagga gggcggtcag gagcgccgtg cgctctacga
     5041 gttggtcgag caggatgtgc tcgtgctcgg cgtgcgcacc ctcgcccggg cagccgaggc
     5101 cgtcgatggt cgcggcgccg acggccgcgg tgaagttgcc gtcgctgccg ccgccggtcg
     5161 aggcctcgcc gaggtcgaga ccgaggctgg cgccgatctc gcgcgcccgc ccgaaggccg
     5221 ccgcgatgcg ctcggtgcgc tccatcggcg gccggttcaa gcccccgctg atcgacagct
     5281 tcgtaccagg aaggcgcggc tgcgcctgga ggatcgagcg cgtgatgcgc tccgcctcgc
     5341 gcgcggtggt cacgcgcacg tcgatctcgg cgcgcgcctc ggccgccacc acgttgcgcg
     5401 cgctgccgcc ggagacgacg ccgacgttga ccgtggtgcc cagcccgtag tcggtcagac
     5461 cgtgcaggta ctggatctga ttggccagct cctggatggc gctgatgccg gcctcgtggt
     5521 cggcgccggc gtgcgcggcg cggccgcgaa tctccatggt gaacatgccg acgcccttgc
     5581 gcgcggtctt gagcgcgccg tgcggtggta ccggcggctc cagcaccagc acgtagtccg
     5641 cctgccgcgc caggtcctcg atgaaggggc gcgagacggg gctgccgacc tcctcctcgg
     5701 tgttgatcaa ccactggatg gcgcggtgcg gcaggtgctg gtcgcggatc aggcgcagcg
     5761 cctccagcat catcgcgacg ctggccttca tgtcgaagac gccgggcccg cgcgccacgc
     5821 gtccctcgac cgtgaacggc ttgcgcgcca gcgtgccgag cggccagacg gtgtcgatgt
     5881 gcgtcagcac cagcaccggt tgcgccgcgc cttgctcggc tttccagttg gccagcgtca
     5941 ggtcgccgta ggccgcctgg ggcaagacct cgaccgcgcc gccgagatcg gcgcagcgct
     6001 cctggagata gcgcgccagg cggtcgaccg ccgtcttgtc gttgctgggc gattccagct
     6061 cgacgatggc ccgcaggctg tcgagcagct cagactcgcg ctggcgtacg tcctcgatcg
     6121 tgaccgccgg caccgcgccg tcctctctat tgttggcccc aggcgtggac caggggcaga
     6181 tcgatcatcg agaccagacc gccggagtgg gccacgacct gcgggatgga gttcatcacg
     6241 atggcggtgg tggcgcggtc gccctgcagg ctcccgggga tgatcagctc cagcggggtc
     6301 ggtccttcga gcatcacgcg gtcctgcgcc gggttggcat ccagatacat cttgaggtgc
     6361 agccggatct tctcctggcc cttgacgaag ccgaggccga gctgatcgac cccggccacc
     6421 tggcccggct tgacgtcgaa gtactgggtg gtgatctggt gatcggccac caccggattg
     6481 gtcgactcgg agtagtcgtc gagctgccag ccgagcgcgg cggcgagcat gaagaccgac
     6541 tcctgcaggc cgacgtggcg cactttaccg gcttcgacca actggcggaa ctcctcgatc
     6601 gtcttgccgg agccgatctt ggcctgcagc ggctgccgcc gggtggaggc gttctgcaaa
     6661 cgctcgatga agaccttgtc gaccttctgg gcgatggcgg tgaagtagat cggcagcgag
     6721 tccatgacga agccggggtt gaccccggtg ccgagcagcg tggctccgcg cgccttgccg
     6781 agctggtcga gctgctgggc cagctcgacc cggttcgcca ccggataggc cagctcctcg
     6841 gttgtcgaga ccacgttcag tcccgctttg aggatgccgt cgagctgcgg catgacctgg
     6901 cgcagcgagg agccggtggt gtggagcacg acgtcggcgt tggccgcttg gaagacctgt
     6961 ccggcgtcgc tgctgaccgt gacgccgaca tcgcggccca gcccgaccac ctggccgaga
     7021 tccttgccga ccttgttggg gtcgatgtcg accgcaccgg cgacctcgat gccggggtgt
     7081 gccagcgcca gccgcgccac ttcctgcccg atcgggccaa gcccgtactg aatcgtgcga
     7141 taggtcttca ccgtcgcgtg tccctcccga tctgctccgg ctccgagcgc gcgctcagga
     7201 gctcttcttc tcttctcgct cgcgcagggt cttgtagtac ttcttggact tgggcatcgg
     7261 cttgctctcc tcggcggcgc gccgcgcgct cgcctccgcg ctggccgagg ccatctgctc
     7321 ttcgagcacc gccaggtcgt cgtagacgcc gacgttggcg ttggcgaccg gggtgcgcat
     7381 caggcggcgc accagctcca actgtgagcc cacctgcgcc cggtcggcgc ccggctggcg
     7441 accgatggtg tcgagcagcc gcatggtctc gactcgcgcg ttgttcgtct gcagccgctc
     7501 gcgaatgcgg tccagggcat ctgccaaata catgcgttgc ctctcttcca gtgcccccgt
     7561 gccaggctgg agtatcccgc ctgatgccga gcggcgcaag tcgcccgcca accggccgcg
     7621 gtgttggacc gtaacttgcc gtagaaagcg accaaccgtg aggtgacggc tgctcgaatg
     7681 acgtggtccc gagatccggc ccgctgggcg cgcgctcgaa ccccaccagc aacagacaag
     7741 aggtttccat gacccttcga tcctctcctc ccgacgaccc cgagaccggg gctttgcgcc
     7801 ggctggtctc cacgcgactg ccgacggccg aggggagctt tcgcctgtgt ctctacgaca
     7861 gcgatgacgg caaggagcac ctggcgctgg tgctgggtga agtcgccgga caggccggtg
     7921 tgctggtgcg cgtccactcc gaatgcttca ccggcgacgt gctcgggtcg ctgcgctgcg
     7981 actgcggcga gcaactgcga cgctcgctgg cgctgatcgc ggccgagggg cggggcgtgc
     8041 tgctctacct gcggcaggag ggacgcggca tcggcctgct cgacaagctc cgcgcctaca
     8101 acctgcagga tcaaggcttc gacacggtcg acgctaacct gttgctcggc caccaacctg
     8161 acgagcgcga ctaccgcgtg gccggcgaga tgctggacga gctggacgtg cgctcgatcc
     8221 gcctgctgac caacaatccg gccaagctgg ccgcgctgcg cgcgctcggc gtgcgcgtcg
     8281 cggggcgggt gccgctccac accgcggcgg ggccggagaa tctggcctac ctcaccacca
     8341 aggcgcgccg catggcgcac caactggagc cggagccgga ggagccgcgc ggcggtcctg
     8401 gagcgcctcc gcgcaacggg caccgccagc gtccggcggt gacactggcc tacgcgcaga
     8461 gtctcgatgg ctccctggct gtgcgctcgg gcgaaccgct ggccctgagc ggtcccgagt
     8521 cgctggtgat gactcatcgg ctgcgcgccg cgcacgacgc catcctggtc ggcatcggca
     8581 cggtgttggc cgacgacccc cgtctgacgg tgcgcctggc gccgggacga cacccgcgcc
     8641 cggtgatcct ggatagccgg ctgcgtttcc ccttggaggc gcgcttgctc gccggcccgc
     8701 ggcggccctg gattgctacg ggtacggaca gctcgcctga acggcaagag gagctggagg
     8761 ccgccggggc gcgggtgctg cgtctgccgc tggacgacgc gggccggatc gcgctaccgg
     8821 cgctgctgcg cgtgctggcc gcgcgcggta tcgagcggct gatggtcgag ggcgggccgc
     8881 gcgtgatcgc cagtttcctg cgccaaaggc tggccgacca gttggtgatt accctggcgc
     8941 cgcgcctggt cggcgggctg cggctgctgg atcagccggg cgattacccg agcctgcgcg
     9001 agccgcgcca ggcgcgcgtc ggccaggatc tggtgct
//
LOCUS       DASUBI010000136         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_677734, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000136 DASUBI010000000
VERSION     DASUBI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677734"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFU72_02605"
     CDS             <1..739
                     /locus_tag="VFU72_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422407.1"
                     /translation="RRLLANVAHELRTPLTSVQGYAQGLSDDVFTTPEAREEALRTIR
                     EESERVNELVLQVLQLARLESGQAEPRPRPVDLDALIARVLRRHRPAAEAAAVSLNEP
                     ARADLSILADESLLEQAIDNLVRNAIRHTPPGGSVGVSVRTAALTGERERRLKIRVSD
                     SGHGIPPDELTHIFDRFYRGSDEAARDGFGLGLAIVREIAFSHGGVVSAESESGRGAT
                     FVIDLPLRLAPAGGTAATGIIEQPARG"
     gene            829..>2387
                     /gene="dxs"
                     /locus_tag="VFU72_02610"
     CDS             829..>2387
                     /gene="dxs"
                     /locus_tag="VFU72_02610"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5422408.1"
                     /translation="MVLERINCPEDLKRLTIPQLETLAQEIRDYIYNVVVKQTGGHFA
                     SNLGTVELTLALHYVFESPKDKLVWDVGHQAYPHKILTGRRDRFPTIRQEGGLSGFLQ
                     REESPHDQFGAGHASTSISAALGMAVAGKLKGERFHTVAVIGDGALTGGIAYEALNNA
                     GSLQLPFIVVLNDNEMSIAPNVGALPKYLSRIRTDERYAQAKAEFERLLQRLPQGDLL
                     VELGKRMKDSFKEFVYHTMIWEELGFTYVGPVDGHNVRDLISTFQDARGLTGPVFIHA
                     VTTKGKGYQPAENDPFKHHSASIKVPGAPPTPPKYQDVFGETLTELARQDERIIAITA
                     AMPDGTGLLPFAKAHPNRFFDVGIAEQHAVTFAAGLATQGLRPVAAIYSTFLQRAYDQ
                     VIHDVCIQKLPVVFALDRAGLVGDDGRTHHGVFDFAYLRCLPNMVVMAPKDEDELRHM
                     LKTAVEHDGPIALRYPRGSGVGVSTASEPHALPIGRAELLREGSDLTIVAIGSTVLPA
                     MHAAEELAARG"
BASE COUNT          420 a          829 c          770 g          368 t
ORIGIN      
        1 gcgccggctg ctggccaacg tcgcccacga gttgcgcacg ccgttgacct cggtgcaggg
       61 gtatgctcag gggctctccg acgatgtctt caccacgccc gaggcgcgcg aggaggcgct
      121 gcgcaccatc cgcgaagaat cagagcgcgt caatgagctg gtgctccagg tactgcaact
      181 ggcgcgcctg gagtcggggc aggccgagcc gcgcccgcgg ccagtcgacc tggatgcact
      241 gatcgcgcgc gtgctgcggc gccaccggcc ggccgccgag gccgccgccg tctccctgaa
      301 cgagccggcc cgggcggacc tctcgatcct ggccgatgag tcgttgctgg agcaggccat
      361 tgacaatctg gtacgcaatg cgatccgcca taccccgccg ggcggcagcg tgggggtgtc
      421 ggtcaggacg gccgcgctga ccggagagcg cgagcgccgg ctgaagatca gggtcagcga
      481 cagcggccat ggcatcccac ccgacgagct gacgcacatc ttcgatcgct tctaccgcgg
      541 cagtgatgaa gccgcgcgcg acggcttcgg tctgggactt gcgatagttc gcgagattgc
      601 atttagtcac ggcggcgtcg tcagcgccga gagcgagtcg ggccgcggcg cgacgttcgt
      661 catcgacctg cccttgcggc tcgcgccagc agggggcacg gccgcaacgg gtataatcga
      721 gcagcctgcc cgaggctaat tgacgcggcg tgcgccggtt gctaggatca cgggcggtgt
      781 ggctgcaagc acgagggcag gtccgcgccc gctcggagga cgaattctat ggtactcgaa
      841 cggatcaact gtcccgaaga cctcaagcgg ctcacgattc ctcaactgga aaccctcgca
      901 caagagattc gcgattacat ctacaacgta gtggtcaagc agacgggtgg gcacttcgcc
      961 tccaacctcg gcaccgtcga actgacactg gcgctgcact acgtcttcga atcgcccaaa
     1021 gacaagctcg tctgggatgt cgggcatcaa gcctacccgc acaagatcct gactgggcgg
     1081 cgcgaccgct tcccgaccat tcgccaggag ggcggcctct cgggcttcct gcagcgcgag
     1141 gagagccccc acgaccagtt cggcgccggc catgccagca cgtcgatctc ggccgcgctc
     1201 ggcatggcgg tggcgggtaa gctcaagggc gagcgcttcc acacggtagc ggtgatcggc
     1261 gacggagcgc ttaccggtgg catcgcctac gaggccctca acaacgccgg cagcttgcaa
     1321 ctccccttca tcgtggtgct caacgacaac gagatgtcca tcgcgcccaa cgtcggcgcg
     1381 ctgccgaagt atctgagccg gattcgcacc gacgagcgtt acgcgcaggc aaaggccgaa
     1441 ttcgagcgcc tcctgcagcg gctaccgcag ggcgacctgc tggtcgaact gggcaagcgc
     1501 atgaaagact ccttcaagga gttcgtctac cacaccatga tctgggagga gctgggcttt
     1561 acctacgtcg gcccggtcga cggccacaac gtgcgcgacc tgatctccac cttccaggac
     1621 gcgcgcggcc tcaccggtcc ggtcttcatc cacgcggtga ccaccaaggg gaaaggctac
     1681 cagccggccg agaacgatcc gttcaagcac cacagcgcct cgatcaaagt gccgggcgcg
     1741 ccgcccacgc cgccgaagta tcaggacgtc ttcggcgaga ccctgaccga gctggcacgg
     1801 caagacgagc gcatcatcgc catcactgcc gccatgcccg acggtaccgg cctcctcccc
     1861 ttcgccaagg cgcaccccaa ccgcttcttc gacgtcggca tcgccgagca gcacgcggtt
     1921 accttcgccg ccgggctggc gacgcagggc ctgcgcccgg tggcggccat ctactcgacg
     1981 ttcctgcagc gcgcctacga tcaggtgatc cacgatgtct gcattcagaa gctaccggtc
     2041 gtcttcgcgc tcgaccgcgc cgggctggtg ggggacgacg gccgtacgca ccacggcgtt
     2101 ttcgacttcg cctacctgcg ctgtctgccg aacatggtcg tgatggcgcc caaagacgaa
     2161 gacgagctgc gccacatgct caagacggcg gttgagcacg acggcccgat cgcgctgcgc
     2221 tacccgcgtg gctccggggt tggcgtctcg accgccagcg aaccacacgc gctgcccatc
     2281 ggtcgtgcgg aactgctgcg cgaggggagc gacctgacca tcgtcgccat cggctccacg
     2341 gtgctccccg cgatgcacgc cgccgaggaa ctggccgcgc gcggcat
//
LOCUS       DASUBI010000137         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_664152, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000137 DASUBI010000000
VERSION     DASUBI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664152"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFU72_02615"
     CDS             <1..998
                     /locus_tag="VFU72_02615"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5422409.1"
                     /translation="LAAALIRTRGDLLRVAGAALAVGCSSGVASIWQHFAKFAAPYAG
                     DLSAAAILTWKGRSLGLAESPVSLANQMVILLAVVIGILACGLGKRRRIRFALIAAFV
                     VLLMGLNFSYTRSAAFGLAPALAAMAFCFRGRRRTTLLAIVVLAVLLFEGLQGTGYIG
                     ARYYKSANNDRSAASHEALWTADLAIALQDPFVGIGHKNFEQASAQFANQANTDITVG
                     ASALGNERPHNDFLSVWLSWGIFALIAYVAIFVGAIRNFLVAIRHQDLLIRGLAVGGI
                     GALIAYGTNSAFHNSMDSSTLLWLLAGLSVSVARIAAEMTVSRLPKTRGLLAGIS"
     gene            1013..2209
                     /locus_tag="VFU72_02620"
     CDS             1013..2209
                     /locus_tag="VFU72_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422410.1"
                     /translation="MQTILYPVNQLRIGGAEQQLLELVRRIDKTRFQAIVAPLYPGGP
                     LDPEFHALPGVEVVELNRRGKADPSPLWRLASLMRRRRVDIVQPFLSPATFFGLLPAL
                     AVRTPVMVVTERCGVRKERGIGYKTYRTVEDWLSRAADALVPNSQAGATMLLERGLPA
                     AKIRVIYNGINLERLEANSAAVAEHRAGLGVPAGGAVAGILASLTQPKGHDVLLRAVA
                     ELAHRQPGLRLAVIGDGPLRAALESQAAELGIADRVTFFGYQRRVADYLAACDLLVSA
                     SRDNEGCSNAILEAMALGVPVIATDIGGNRELVGHRETGYLVPVGDAPALADTIDGLL
                     QYPLEAQRIVSAARAMVHQRFSLERMVADYEALYTRLLSHDRVPVRQVANEAGSLEQS
                     VSAAEG"
BASE COUNT          362 a          775 c          806 g          437 t
ORIGIN      
        1 tactggcggc ggcgctgatc cgcacgcgcg gcgacctgct gcgcgtagca ggcgccgcgc
       61 tcgccgtcgg ctgctccagc ggcgtcgcgt ccatctggca gcacttcgcc aagtttgctg
      121 cgccttatgc cggcgacttg tcggccgccg caatactcac ctggaaaggg cgctcgctgg
      181 gtcttgcgga aagcccagtt tctctggcca atcagatggt gattctcctc gccgtagtca
      241 tcggtattct ggcctgcggc ttaggcaagc gacgtcgcat ccggttcgcg ctcatcgctg
      301 cgtttgtcgt tctgttgatg ggattgaatt tctcctacac tcgatcggcg gccttcggtc
      361 tggcgcccgc gctcgcggcg atggccttct gctttcgagg gcggcgtcgc acaacgcttc
      421 tggcgatcgt ggtgctcgcc gtgctgctct tcgaaggact ccagggcacc ggctacatcg
      481 gcgcgcgcta ctacaagtcg gccaacaacg accggagcgc cgcctcgcac gaggcgctgt
      541 ggaccgccga tctggcgatc gcgctgcagg atccgttcgt cggcatcggg cacaagaact
      601 tcgaacaggc ctccgcacaa ttcgcgaatc aggccaatac cgatatcacc gtcggcgcgt
      661 cggccctggg caacgagcgc ccgcacaacg acttcttgag cgtgtggctc tcctggggca
      721 tcttcgcctt gatcgcctat gtcgccatct tcgtgggcgc gattcgcaat tttctggtcg
      781 cgatccggca ccaggacctg ctcatccgcg gcctggcggt cggcggtatc ggcgcgctga
      841 tcgcctacgg caccaactcg gcctttcaca actcgatgga cagcagcacc ctgctgtggc
      901 tcctggccgg cctgtcggtc tcggtcgcgc ggatcgcggc cgagatgact gtgagccgtt
      961 tgcccaagac gagagggctg cttgctggaa tcagctagga ggggtggacg gcgtgcagac
     1021 gattctctat ccagtcaatc agcttcgtat cgggggagcc gagcagcagt tgctggagct
     1081 ggtgcggcgc atcgacaaga cgcgctttca agcgatcgtg gcgccgttgt atccaggcgg
     1141 cccgctcgat cccgaatttc atgccctccc gggcgtcgag gtcgtcgagc tcaaccggcg
     1201 cggcaaggcc gacccgtcgc cgctctggcg gctggcttcg ctgatgcgcc ggcgccgcgt
     1261 cgacatcgtg cagcccttcc tcagcccggc cacgttcttt ggtctcctgc cggccctggc
     1321 ggtgcgaacg ccggtgatgg tggtgaccga gcgctgcggt gtgcggaaag agcgaggtat
     1381 cggctacaag acctaccgca cggtggagga ctggctgagt cgagcggccg acgcgctggt
     1441 gccgaacagc caggccggcg cgaccatgct gctggagcgg ggcctgccgg ccgcgaagat
     1501 ccgcgtgatc tacaacggca tcaatctgga gcggctggag gccaactcgg ccgccgtcgc
     1561 cgagcaccgt gccggcctcg gcgtgccggc ggggggtgcc gtagccggca tcctggccag
     1621 cctgacgcag ccgaagggac atgacgtgct gctacgcgcg gtagccgaac tggcgcatcg
     1681 ccagccgggt ctgcgcctgg ccgtgatcgg cgacggaccg ctgcgcgccg cgctggagtc
     1741 acaagcagcg gagctcggga tcgccgaccg ggtgacgttc ttcggctacc agcgccgcgt
     1801 ggcggattac ctagcagcct gcgacctgct tgtctccgcc tcgcgcgaca acgagggttg
     1861 ctccaatgcg attctggagg ccatggcgct cggcgtgccg gtgatcgcca ccgacatcgg
     1921 cggcaaccgc gagctggtgg gccaccgcga gactggctat ctcgtccccg tcggtgacgc
     1981 cccggcgctg gcggacacga tcgacggcct gctgcagtac ccgctagaag cccagcgcat
     2041 cgtaagcgcg gcacgcgcga tggtgcacca gcgcttcagc ctggagcgca tggtggcgga
     2101 ctacgaggcg ctctataccc gcttgctgag ccacgatcgg gtgccggtgc gccaggtcgc
     2161 gaatgaggct ggctcactgg agcagagtgt ttcagcggcc gagggctagc tgggctgata
     2221 cggactgcgt cgagctgcaa acagattctc aacggtctct gcacccccgc gagatgatcg
     2281 atgcctagca tggtgggaaa gaaacctgcg ggtttgaact tgccgggcga gcgtgcgcgg
     2341 tggtggttcg gggtcccgaa ccgggattga tgatttcaca
//
LOCUS       DASUBI010000138         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_854784, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000138 DASUBI010000000
VERSION     DASUBI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854784"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFU72_02625"
     CDS             <1..1120
                     /locus_tag="VFU72_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422411.1"
                     /translation="IGLTTKLLFIWFLIAIPAAYAILLAADWLAAPSRRPLRDSWARL
                     RADLPLSSPLDLVAAGGGFLIGAAPVIYYNLVSRGSYLVFRANLTQTDRGVDNFALWH
                     NAKVELDAVRVLLDGGYFWFYGGIYTNRLWPWFAGLAALGLLLLTHAAPELRRYRRAT
                     VFLLAFSAVIFLLSCFTVSILGPTHLLILLPIPQLLVAAFALLGGRWLAARLPRPLRL
                     APPALAALLLVTLAAGDLLVDARYHQALARTGGYTNFSSAIYTLADQLEAEGDTRPYA
                     LDWGFKYNLEIISDGRVVPQEIYGATYEPGPDFEAAVRRALADPNAVFIAHVEGSESF
                     PRLDAFRRIVAESGRQLVLVRTDKELSGRPAYYEFRVK"
     gene            1613..3262
                     /locus_tag="VFU72_02630"
     CDS             1613..3262
                     /locus_tag="VFU72_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEU5422412.1"
                     /translation="MARLRLIQFRAKIQRLPLFRLALLLILIGALGLSWLADGYFNRG
                     VASGANLTPIPNTDVNPLGVNTLMNDDDPATIDRTLDMVAAGGFTYVRQMFPWYAIEP
                     VKDQFQWAQWDRIVAAADARHLELIVRLDKPPRWARAGQPNVDQVPDGPPNDDADYAR
                     FVAAVVSRYRGKIHYIQIWNEPNLAGEWGGLPIDPARFARLLETAYRAAKQADPAITV
                     LLPGLAPTDAKGPDNLSDLTYLQDLYAAGAKNSFDIVSAMVYGYGYSPGDRRVSFDRD
                     NFSRPIQTHEIMVRNGDGAKPVWAAEYGWVSLPKDWQGQPSPWGKPVSLKTQAEYLEQ
                     GYLRAQKEWPWMGVMCVWDFRWPYDPSLPDQAGNPTRGFSLVNYDFTPNPAYTVLSQA
                     RSLLDRAYTGAYRADTRLIQHDDGWALAGTRLTAARDGATLRIPFSGTRLDLLTSGDG
                     GFAVTLDGQARPAVPAGGRVTVAKGLSDGPHLLQLTARAGVPALDGFVVARRPLSAWI
                     FPYVYGALGVLILLDLLGLARVLRRARRRRALPVGQALAEH"
     gene            3291..3704
                     /locus_tag="VFU72_02635"
     CDS             3291..3704
                     /locus_tag="VFU72_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5422413.1"
                     /translation="MSGPADAGNGEIVRQFDSAWNSHDVDQVMRLFADNPLVELRPAI
                     PGLPDTYRNTDQLRMMLQALLPGSHVESQGARAQGNDVTWQASLSSDTFRQLGLATLT
                     SDLQATLTQQVKIERLAVLFPPDLVALIQGAPKTR"
     gene            complement(3897..>4427)
                     /locus_tag="VFU72_02640"
     CDS             complement(3897..>4427)
                     /locus_tag="VFU72_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422414.1"
                     /translation="KGLGAGNPRSEPDYHAYGIPAGGLGERISRLGESCRVLKLLWTE
                     EEASFAGRYYQLDHARPAIWPVQRPHPPLWIGGHGDRVLRMTARHADRWNYVGPFEGF
                     VDAVPRLRAACEQVGRDITTLTITAQISVDALPAAEARATMARYQEAGAQMLIAALPL
                     PYTEQRVTALAEKLFG"
BASE COUNT          678 a         1613 c         1444 g          692 t
ORIGIN      
        1 catcgggctg acgaccaagc tcctcttcat ctggttcctg atcgccatcc ccgccgccta
       61 cgcaatcctg ctggccgccg actggctcgc tgctccctcg cgccggcccc tgcgcgactc
      121 ctgggcacgg ctgcgggccg acctgccgct gtcgtcgccg ctcgatctgg tcgcggccgg
      181 cgggggcttc ctcatcgggg ccgccccggt catctactac aacctcgtca gccgcggcag
      241 ctacctggtc ttccgcgcca acctcaccca gaccgaccgc ggggtggaca atttcgccct
      301 ctggcacaac gccaaagtcg agctcgacgc ggtgcgcgtg ctgctcgacg gcggctactt
      361 ctggttctac ggcggcatct acaccaaccg gctctggccc tggtttgccg ggctcgcggc
      421 gctgggtctg ctgctgctga cccacgccgc gccggagctg cgccgctacc gccgcgccac
      481 cgtcttcctg ctcgccttca gtgcggtgat cttcctcttg agctgcttca ccgtctcgat
      541 cctcggtccg acgcatcttt tgatcctgct gccgatcccg cagctcctgg tcgcggcctt
      601 cgccctgctc ggcgggcgct ggctggcggc tcggctcccc cgcccgctgc gcctggcgcc
      661 gccggcgctg gccgcgctgc tgctggtgac gctggcggcc ggcgacctgc tggtcgacgc
      721 gcgctaccac caggcgctgg cgcgcaccgg cggctacacc aacttctcca gcgcgatcta
      781 caccctggcc gatcaattgg aggcggaggg ggatacgcgc ccctacgcgc tcgactgggg
      841 cttcaagtac aacctggaga tcatcagcga cgggcgcgtg gtgccccagg agatctacgg
      901 cgccacctac gagccggggc cggatttcga ggccgccgtg cgccgcgcgc tggccgaccc
      961 caacgcggtc ttcatcgccc acgtcgaggg ctcggaatcc tttccgcgcc tggacgcctt
     1021 ccgccgcatc gtggccgaga gcggccggca actggtgctg gtgcgtactg ataaagagct
     1081 gagcggacgg cccgcctact acgagttccg ggtgaagtaa catgacgcgt gcagtggcat
     1141 tcgcggcgcc ggtcgggcgg gaataatgga gttcgagtaa ttagcccgtg ctcggtcgcc
     1201 cgtgtcgcgc tgggctagag ccgcggcgct agcagacgaa gcctattgat ggagtttgac
     1261 gaattgatcc gggacattgg tgccggccgg cgcgcggggc taaagccgcc gcgctaagtc
     1321 ctacgaagcc cactgaaggg gctgggcagg aacggaaaaa acgccaaact atttcgtcaa
     1381 acgccctaaa caggctgagg accgcgacgg ggccgcacct gattactcca gcatattctg
     1441 tgaagcaagt tgagtattgt gcaatcttca gcctgcttca gcaggctttc gtgaagatct
     1501 agcgctgcgg ctttagccca gcgcggcacg ggcgatggag cacggggcta attgctcaaa
     1561 ctccataaac caccgcgcta acacagagga agcccgctaa aggggctgcg gtttggcacg
     1621 cttgagactg atccagttcc gcgcaaagat ccagcggctc ccgctcttcc ggctggcgct
     1681 gctgctgatc ctcatcggcg cgctcggcct ctcgtggttg gccgacggct acttcaaccg
     1741 cggggtggcc agcggcgcga acctgacgcc gattcccaac accgacgtca acccgctggg
     1801 cgtcaacacg ctgatgaacg acgacgaccc ggccaccatc gaccgcacgc tcgatatggt
     1861 cgcggccggc ggcttcacct acgtgcgcca gatgttcccc tggtacgcca tcgagccggt
     1921 gaaagaccaa ttccagtggg cgcagtggga ccgcatcgtg gccgccgctg acgcccgcca
     1981 cctggagctg atcgtgcggc tcgacaagcc gccgcgctgg gcgcgcgccg gccagcccaa
     2041 cgtcgaccag gtgcccgacg gcccccccaa cgacgacgcc gactacgccc gcttcgtggc
     2101 cgccgtggtc agccgctacc ggggcaagat ccattacatc cagatctgga acgagcccaa
     2161 cctggccggc gagtggggcg ggctgccgat cgacccggcg cgcttcgccc gcctgctgga
     2221 gaccgcctac cgcgccgcca agcaggccga ccccgccatc accgtccttc tccccggcct
     2281 ggcgcccacc gacgccaaag gaccggacaa cctcagcgac ctgacctacc tgcaagacct
     2341 ctacgccgcc ggggccaaga actccttcga catcgtcagc gccatggtct acggctacgg
     2401 ctacagcccc ggcgaccgcc gcgtcagctt cgaccgcgac aacttctcgc gccccatcca
     2461 gacacacgag atcatggtgc gcaacggcga cggcgccaag ccggtctggg ccgccgagta
     2521 tggctgggtc tcgctgccca aggactggca ggggcagccc tccccctggg gcaagccggt
     2581 cagcctcaag acccaggccg agtacctgga gcagggttac ctgcgtgcgc aaaaagagtg
     2641 gccctggatg ggggtcatgt gcgtctggga cttccgctgg ccctacgatc ccagcctgcc
     2701 cgaccaggcc ggcaacccga cgcgcggctt ctcgctggtc aactacgact tcacgcccaa
     2761 cccggcctac accgtgctca gccaggcacg gtcgctgctc gatcgcgcct acaccggcgc
     2821 ctaccgcgcc gacacgcgcc tgatccagca cgacgacggc tgggcgctgg ccggcacgcg
     2881 cctgaccgcc gcgcgcgacg gcgccacgct gcgcatcccc ttcagcggca cccggctcga
     2941 tctgctgacc agcggcgacg gcggcttcgc ggtcacgctc gacggccagg cgcgtccagc
     3001 cgtgccggct ggcgggcgcg tcaccgtcgc gaagggcctc tccgacggcc cgcacctgct
     3061 gcagctgacg gcgcgcgctg gagtccccgc gctcgacggc ttcgtggtgg cgcgccggcc
     3121 gctgagcgcc tggatcttcc cctacgtcta cggcgcgttg ggagtcttga tcctgctcga
     3181 cctgctcggc ctggcccgcg tgctgcggag ggcgcggcga cgacgggcgc ttccggtagg
     3241 gcaggcgctc gccgagcatt gagaaccggt ttagcggaga gggacgggcg atgagcggac
     3301 cagcggacgc gggcaacggc gagatcgtgc ggcaattcga cagcgcctgg aacagccacg
     3361 acgtcgacca ggtgatgcgc ctcttcgcgg acaacccgct ggtggagctg cgcccggcca
     3421 tccccggtct gcccgacacc tatcgcaaca cggatcagct tcgcatgatg ctgcaggcgc
     3481 tgctgccggg ctcgcacgtc gaatcgcagg gcgcgcgggc gcaggggaac gacgtgacct
     3541 ggcaggcgag cctctcctcc gacaccttcc gccagctggg gctcgccacg ctgaccagcg
     3601 acctgcaggc cacgctgaca cagcaggtca agatcgagcg cctggccgtg ctcttcccgc
     3661 ccgatctcgt cgcgctgatc cagggcgcgc cgaagacgcg gtagggccgc cgccttcgaa
     3721 tgttttgggt gccgcgcggg gcacgaatcc ttcgactgcg ctcaggatga cagggggaga
     3781 ggatgcggcc aatcggacga ggcaaatcct cctcgtccgc ccctctctta ccctgtcatc
     3841 ctgagcttgt cgaaggatcc gtcctcacag gccaccgacg ctcgtcgcaa gaagcctcag
     3901 ccgaagagct tctcggccag cgccgtgacc cgctgctcgg tgtaggggag cggcagcgcg
     3961 gcgatcagca tctgcgcgcc cgcctcctgg tagcgggcca tcgtcgcccg cgcctcggca
     4021 gccggtagcg cgtcgaccga gatctgcgcc gtgatggtca gcgtcgtgat gtcgcgcccg
     4081 acctgctcgc aggcggcgcg cagccggggg actgcatcga cgaagccctc gaagggaccg
     4141 acgtagttcc agcggtcggc gtggcgcgcg gtcatgcgca gcacgcgatc gccgtggccg
     4201 ccgatccaga gcggcgggtg cggccgctgc accggccaga tcgccgggcg cgcgtgatcg
     4261 agctggtagt agcggccggc gaagctggcc tcctcctcgg tccagaggag cttcagcacg
     4321 cggcacgact cgcccaggcg gctgatgcgc tcgcccaggc cgccagccgg gatgccgtag
     4381 gcgtggtagt ccggctccga gcgcgggttg ccggcgccca gcccctt
//
LOCUS       DASUBI010000139         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_145910, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000139 DASUBI010000000
VERSION     DASUBI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145910"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1231
                     /locus_tag="VFU72_02645"
     CDS             11..1231
                     /locus_tag="VFU72_02645"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEU5422415.1"
                     /translation="MAEGALRGVMRRPMVLKRFVNGAAEEPFFQKRAPQNLPEGIETA
                     RVTFPSGRWADLAVADDAADLLWAVNLGCIDLNPWPVRANDVDHPDELRIDLDPTPEA
                     TFEHVKQTAMLVKETLEDFGYRGFAKTSGSRGIHIYVRIEPRWEFQQVRRAGLALARE
                     IESRNPALVTTAWWKEERHGVFIDYNQNARDRTIASAYSVRPTPNAQVSCPLDWEEVP
                     GVELADFTIETVPRRFAEMGDPMAEIDDKLFSLEPLLELVEKQERAGQGDAPWPPQFP
                     KGEAEPRRVQPSRARKDGAAPKSGTGRRQSSEPLITIAKAQHKAQALDGLERWKQRHP
                     EAAPHIEAGDVLVDSMRGMSSTWTRIRVNLRHVPEDERPAEEAPDPDYDPWANVPAEL
                     RGRHTRFNPADGRL"
     gene            1391..>2050
                     /locus_tag="VFU72_02650"
     CDS             1391..>2050
                     /locus_tag="VFU72_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422416.1"
                     /translation="MSGSSNMQETSVAPADRSVAAIPLPRIDAQAVCLIVTTLIVGAL
                     ALLKTTDPDYWWHLRTGQLIAQTHSVPTVDSYSFTANGKSWLAHEWLSELIIYGLDQA
                     GGYALSILVFLAVAAGALWLGFRTALALGASRWAALLTFIWATFMFGGFWVVRPQVFS
                     WLFLALFLAIIVEHRRGKASLWWLVPLTAVWVNLHLGVMFGLAMVAIYAVSLGVERLL
                     WH"
BASE COUNT          330 a          669 c          727 g          324 t
ORIGIN      
        1 ctacgaggcg gtggcggagg gggcgctgcg aggcgtgatg cggcggccga tggtgctcaa
       61 gcgcttcgta aacggcgccg ccgaggagcc gttcttccag aagcgggcgc ctcagaacct
      121 gccggagggc atcgagacgg cgcgggtgac gttccccagc ggtcgctggg ccgacctcgc
      181 ggtcgcggac gacgctgccg acctgctatg ggccgtgaac ctgggctgca tcgacctgaa
      241 cccgtggccg gtgcgcgcca atgacgtcga tcaccccgac gagctgcgca tcgacctcga
      301 cccgacgccc gaggccacgt tcgagcacgt gaagcagacg gcgatgctgg tcaaggagac
      361 gctggaggac ttcggctatc gcggctttgc caagacctca ggctcgcgcg gcatccacat
      421 ctacgtgcgc attgagccgc ggtgggagtt tcagcaggtg cgtcgcgccg ggctggcact
      481 ggcgcgggag atcgagagtc gtaatccggc gctggtgacg accgcgtggt ggaaggaaga
      541 gcgccacggc gtgttcatcg actacaacca gaacgcgcgc gaccgcacga tcgcgtccgc
      601 gtactcggtg cggccgacgc cgaacgcgca ggtctcgtgc ccgctcgact gggaagaagt
      661 gccgggcgtc gagcttgccg acttcacgat cgagaccgtg ccgcggcgtt tcgccgagat
      721 gggcgacccg atggccgaga ttgacgacaa gctattttcg ctggaaccgc tgctggagct
      781 ggtcgagaag caggagcgcg cggggcaagg cgacgcgccg tggccgccgc agtttcccaa
      841 gggcgaggcc gagccgaggc gagtgcagcc cagtcgcgcg cgcaaggacg gcgccgcacc
      901 gaagagcggt acggggcgcc gccagtccag cgagccgctg atcacgatcg cgaaggcgca
      961 gcacaaggca caggcgctgg acggcctcga gcgctggaag cagcgccatc ccgaggcggc
     1021 gccgcatatc gaagccggag acgtgctggt cgattcgatg cggggcatgt cttcaacctg
     1081 gacgcgcatc cgcgtcaacc tgcgccatgt gccggaggac gagcgcccgg ccgaagaggc
     1141 cccggacccg gactacgacc cgtgggccaa cgtgccggcg gagctgaggg gccgccatac
     1201 gcgcttcaat ccggcggacg ggcggctgta agggcgcggc acagggattc tcctcagtac
     1261 ggaaggatgg cggccgcgcc gaaccttaga accagggaac cagggaacca ggggaacagc
     1321 gcctccatgt cctgcctgtg actccgcctt gccgccgtgg cgccgagtat tacagtggag
     1381 ccgcctggtc atgagcggct catcgaatat gcaagagacg tctgtagctc ccgccgaccg
     1441 tagcgtggcc gccatcccgc tgccgcgcat cgacgcgcag gccgtgtgcc tcatcgtgac
     1501 gacactgatc gtcggggcgc tggcgctgct caagacgacg gaccccgact actggtggca
     1561 cctgcgcacc ggacagctca tcgctcagac gcactcggtg ccgaccgtcg acagctactc
     1621 gttcaccgcg aacggcaagt cgtggctggc ccacgagtgg ctctcagagc tgatcatcta
     1681 cggtctcgac caggccggcg gctacgcctt gagcattctc gtgttcctcg ccgtcgccgc
     1741 cggggcgttg tggctcgggt tcaggaccgc gctggcactc ggggcctcgc gctgggcggc
     1801 gctgctcacc ttcatctggg ccacgttcat gttcggcggc ttctgggtcg tgcggcccca
     1861 ggtctttagc tggctgttcc tggcgctctt cctcgcgatc atcgtcgagc accggcgcgg
     1921 caaggcgtcg ctctggtggc tcgtgccgct taccgccgtg tgggtcaacc tacacctcgg
     1981 cgtgatgttc gggctggcga tggtggcgat ttatgccgtc agtcttgggg tcgagcgctt
     2041 gctgtggcac
//
LOCUS       DASUBI010000140         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_992533, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000140 DASUBI010000000
VERSION     DASUBI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992533"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..140
                     /locus_tag="VFU72_02655"
     tRNA            66..140
                     /locus_tag="VFU72_02655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Met,seq:cat)
     gene            310..1548
                     /gene="glgA"
                     /locus_tag="VFU72_02660"
     CDS             310..1548
                     /gene="glgA"
                     /locus_tag="VFU72_02660"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEU5422417.1"
                     /translation="MSTLDRVAIFTNEYPPNVYGGAGVHVEYLSRALARLLPVEVRCF
                     GDQNLVEGDLWVRGYQPWEALTQNTDPRFTGALDAFSRDLAMAKDWLDAGVVHCHTWY
                     TDMAGFLAKTLWNIPLVVTIHSLEPLRPWKVEQLGAAYHLSSWMERVGIEAADAVVAV
                     SHETRRDVLRHFAISPDNVHVIHNGIDLQEYHRTPRVEALTRYGVDPERPYVLFVGRI
                     TRQKGIIHLVDAIPQIDPALQVVLLAGAPDTQEIAREMAERVEEAQTARPNVVWIDAM
                     LPRPEVIEFFSHAAVFCCPSVYEPFGIINLEAMACETAVVASDVGGIPEVVVPDETGL
                     LVHLELKPGTFEPVDPARYAHELATAINTVGLNPELRQTFGRNGRRRVEQSFSWDSIA
                     QQTLALYQRLVAARSEQVTS"
     gene            complement(1538..2215)
                     /locus_tag="VFU72_02665"
     CDS             complement(1538..2215)
                     /locus_tag="VFU72_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422418.1"
                     /translation="MLCPNCSEPQLPGARRCPNCGARVSSPGTVRRRRRSSAGGVLVF
                     WAGLGVVALVGLLVLPGVIGAGSHRVAAGIEARIPRADASGGAVFSATPVSLVSSGAS
                     SVVADATATRVVLTAQDLNARVAEHAQELQPLDHVRIDVTGAGIVVDFGAYNLSGTYR
                     GGLTVVDGVPLVTGGKISGVLGWFVTPNQLESALNQEIAAAVSAQGVWVEQVETQPGE
                     LIFYVKT"
     gene            2373..2942
                     /locus_tag="VFU72_02670"
     CDS             2373..2942
                     /locus_tag="VFU72_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEU5422419.1"
                     /translation="MATTERYTMDQFVADAKRVMGAANGDRQRVVRELAPLVERVVWD
                     DGLFDVRYRAEPEGGRPRYLYHTEPDGSLQVYMVEFAPGMPTPVHDHVTWGLIGICGG
                     EQKTSRYSRLDDGSNPEQAELKPIGEETLGRGAVYPLLPPEDIHRIETVGDRPSYSLH
                     VLGADLRRQHRHIFDVESGSVTAVDGRGM"
BASE COUNT          489 a         1116 c         1010 g          446 t
ORIGIN      
        1 ccgtgggaga cgcggcctcc gcgcgcaccc cttacccgct cacccggctc acccaaaccc
       61 agcagcgcgg ggtggagcag tggcagctcg ttgggctcat aacccaaagg tcgcgggttc
      121 gaatcccgcc cccgctacca acacgacgca ccgttcgatc tcgaacggtg cgtctttcgt
      181 tttaaccccc caaccccctg gctagcgcgc cgcacgggcg gcagtacaat cgccgcgttg
      241 gcagccgggc tgcgatccgg cggggtctcc cggcggtggg ccgggatcgt gctcagacag
      301 ggacacctcg tgagtacact cgatcgcgtc gccatcttca ccaatgagta cccgccgaac
      361 gtctacggcg gcgccggggt ccacgtggag tatctcagcc gcgcgctcgc ccgcctgcta
      421 ccggtcgagg tgcgctgctt cggcgatcag aacctggtcg agggcgatct ctgggtgcgc
      481 ggctaccagc cctgggaggc gctgacgcag aacaccgatc cgcgcttcac cggcgcgctc
      541 gatgccttct cgcgcgatct ggccatggcc aaggattggc tcgacgccgg cgtggttcac
      601 tgccacacct ggtataccga catggccggc ttcctggcca agacgctctg gaacatcccg
      661 ctggtcgtca ccatccactc actcgagccg ctgcgcccct ggaaggtcga gcagctcggc
      721 gccgcctatc acctgagctc ctggatggag cgcgtcggca tcgaggccgc cgatgccgtg
      781 gtagcggtct cccacgagac gcgccgcgac gtgctgcgtc acttcgccat ctcgccggac
      841 aacgtccacg tcatccacaa cggcatcgac ctgcaggagt accaccgcac gccgcgcgtc
      901 gaggccctga cacgctatgg cgtcgatccc gagcgcccct acgtcctctt cgtcggccgc
      961 atcacgcggc agaaggggat tatccatctg gtcgacgcga ttccgcagat cgacccggcg
     1021 ctccaggtgg tgctgctggc cggcgcgccg gatacgcagg agatcgcgcg cgagatggcc
     1081 gagcgcgtcg aggaagcaca gacggcacgc cccaacgtcg tctggatcga cgccatgctg
     1141 ccgcggccgg aggtgatcga gttcttctcg cacgccgccg tcttctgctg tccctcggtc
     1201 tacgagccct tcgggatcat caacctggag gcgatggcct gcgagacggc ggtcgtcgcc
     1261 tcggatgtcg gcggcatccc cgaggtagtg gtgccggacg agaccggcct gctggtgcac
     1321 ctggaactca agccgggcac cttcgagccg gttgaccccg cgcgctatgc gcacgagctg
     1381 gcgacggcca tcaacacggt cggtttgaac cccgagctgc gccagacctt cgggcgcaac
     1441 ggccgccgcc gcgtggagca gtcgttcagc tgggacagca tcgcccagca gacgctggcg
     1501 ctctaccagc ggctggtggc ggcccgctcg gagcaggtta cgtcttgacg tagaagatca
     1561 gctcgcccgg ctgcgtctcg acctgctcca cccagactcc ctgcgccgag accgcggccg
     1621 cgatctcctg attcagcgcc gactcaagct gattgggcgt cacgaaccag ccgagcactc
     1681 cgctgatctt gccgccggtc accagcggca cgccgtcgac caccgtcagc ccgccgcgat
     1741 aggtgccgct gagattgtag gcgccgaagt cgaccacgat cccggcgccg gtgacgtcga
     1801 tgcgcacatg gtcgagcggc tgcagctcct gggcgtgctc cgccacgcgg gcgttgagat
     1861 cctgcgcggt cagcacgacg cgtgtcgccg tggcatcggc caccacgctg ctcgcgcccg
     1921 aggagaccag ggagaccggg gtggcgctga agacggcgcc gccgctggca tcggcccgtg
     1981 gaatcctggc ctcgatcccc gcggcgacgc gatgcgaccc cgccccgatc acccccggca
     2041 gcacgagcag ccccaccagc gcgacgacac ccagcccggc ccaaaagacc agcacgccgc
     2101 cggccgagct gcgccgccgc cgtctgaccg tgcccggcga ggagacgcgc gccccgcaat
     2161 tggggcagcg gcgcgccccc ggcagttgcg gttcggagca attcgggcag agcatgcgca
     2221 cctcccggtt cgagcggagc ggcgtgtcgt ggctacgtcg tgcagagtcc aacgccgctg
     2281 actatcccgt gtgctcctcg ctcgcttcga gtataacggt agccggacat tctggggcca
     2341 gcgccggcac gaggcaggat ggggagacgc gaatggcaac caccgagcgc tacacgatgg
     2401 atcagttcgt ggccgacgcc aagcgggtga tgggcgcggc caacggcgac cggcagcgcg
     2461 tcgtgcgcga gctcgcgccg ctggtggagc gcgtcgtctg ggacgacgga ctcttcgacg
     2521 tgcgctaccg cgccgagccg gagggcggcc gcccgcgcta cctctaccac accgagccgg
     2581 acggctcgct gcaggtctac atggtcgagt tcgcgccggg catgccgacg ccggtgcacg
     2641 accacgtcac ctggggcctg atcgggatct gcggcggcga gcagaagacc tcgcgctaca
     2701 gccgcctcga cgacggaagc aaccccgagc aggccgaact gaagccgatc ggggaggaga
     2761 cgctgggccg cggcgcggtc tatcccctgc tgccgcccga ggacatccac cgcatcgaga
     2821 cggtcggcga ccggccgagc tactcgctgc acgtcctcgg cgccgacctg cgccgccagc
     2881 accgccacat cttcgacgta gagagcggca gcgtcaccgc ggtcgacggc cggggaatgt
     2941 aggcgcgcag gcggttccgc ccgcagcacc ctccagcaga gggaagatca acaccccgct
     3001 ccgactgctc ggagcggggc atctcattta gccctctgat cgccgggcct ccacgctgga
     3061 t
//
LOCUS       DASUBI010000141         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1344485, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000141 DASUBI010000000
VERSION     DASUBI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344485"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2104)
                     /locus_tag="VFU72_02675"
     CDS             complement(80..2104)
                     /locus_tag="VFU72_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5422420.1"
                     /translation="MHEQEPPREPGEPAEERPREAIRGRRPGDRHIRVIRSTELRPPA
                     SPTYLVAEPSAAKSSFGRAYRRVHRVLIGAPIPTAHEIHERLTKVKALAVLSSDAISS
                     TAYATEETLLILVLAGSVAFQYVIPIGIAIIALLAIVASSYRQTIRAYPQGGGSYIVT
                     KDNLGTGPSLLAASSLLIDYTLTVAVSISSGVAAITSAFDSLAPYRVELAVGAIVFIT
                     LANLRGVRESGSIFAVPTYLFIVGMFAVILLGLAAAWFHLFTPHPVTHSIPPATKALT
                     IFLVLRAFSSGCTALTGVEAISDGVPAFQPPEARNARRTLTAMALVLGSLFLGITILT
                     YHFHLVPSANETILSQLTRTVVGRSPFYYYVQATTAGILLLAANTSFSDFPRLSFFLA
                     RDGFLPHQFRLRGDRLAYSTGILALGIASSILVIGFGASVNALIPLYAVGVFLAFTLS
                     QASMTYRWWKREPKGHQRNSGMLINGLGALTTGVVAVIIITTKFLTGAWIVLLLLPIL
                     IATMLAIHRHYQRVAAQLVLPPQEHRGAANWPRESAAIVPIDALNEASMRAIEYAERI
                     ASDVVVVHVALDTKEAEQLAEQWRRERMELPLVVIESPYREIVGPLVSYIEQAHAEKG
                     GPTLTVVLPEFVPAHLYELVLHNQTTLRLKLALWRHPGIVVTNVPYLLTV"
     gene            complement(2143..2535)
                     /locus_tag="VFU72_02680"
     CDS             complement(2143..2535)
                     /locus_tag="VFU72_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5422421.1"
                     /translation="MSGSFAAEFNPAVSHTALKVSDLEAALRFYRDTIGLPFVRSQGP
                     EERPAVVWLRGLQLVRRTPEEIGERGALDHIGLGIDNIEAVCARVEQVGTPVETALEH
                     RVLPGGQEVMVAFYRDPEGNRVELLKYL"
     gene            2808..3806
                     /locus_tag="VFU72_02685"
     CDS             2808..3806
                     /locus_tag="VFU72_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422422.1"
                     /translation="MFETYIGANGGVFRLSGAKLEPLGLEGKRVSAVYAWRDNGSTVV
                     LAGCYGDGIFRSQDGGQHWSEANQGLSAPVLRMIQADPTHPGALICGTEPARAFRSSD
                     GGRSWQELGGVTDLENYQEWYLPYSPRAGALRNFFSPPGQSDRLLGAIEVGGLIDSRD
                     GGKTWSYVDVGPDHDIHWVTGDPHEPNRLFAALGYARMPWQRELPHMPRFGGVGRSRD
                     GGATWEKLLTDYTRAVLVLPERPNMVLAGPSPEVGRQGRIEVSVDGGDHWLPASDGIS
                     VPMEDMVERFYLAPDGHVWAVTSGGRVFTANPDDWQWQAPLPGGQPVEAESVSFVA"
     gene            complement(3803..4087)
                     /locus_tag="VFU72_02690"
     CDS             complement(3803..4087)
                     /locus_tag="VFU72_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422423.1"
                     /translation="MMQPAPTIVSEIAGVRTSTLQVTYRGRTFQAVIRADWSSLQPSY
                     TLQLLELGPRGVPEPLHGASQAGLAGYGEAREAARNAIAALVDGCAVTSD"
     regulatory      complement(4248..4337)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4535..>4740
                     /locus_tag="VFU72_02695"
     CDS             4535..>4740
                     /locus_tag="VFU72_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422424.1"
                     /translation="MLGIGELGRLVGIKHRGRPAPPSPARADWLDRFPWVLLRLARAG
                     SGHAVGTLSVWLWWDRFAAHYWHIR"
BASE COUNT          732 a         1569 c         1742 g          697 t
ORIGIN      
        1 ctgggcgagg ggagacgaag cttaactccc cttctcccag cattgggaga agggggcggg
       61 gggatgaggg ccgcccgcac tacaccgtca ggagatacgg cacgttggtg acgacgatgc
      121 cggggtggcg ccagagcgcg agcttcaggc gcagcgtcgt ctggttgtgc aacaccagct
      181 catacaggtg cgccggcacg aactccggca agaccaccgt cagcgtcggg ccgcccttct
      241 cggcgtgcgc ctgctcgatg tagctgacca gcggcccgac gatctcgcgg tagggtgact
      301 cgatgaccac cagcggcagc tccatgcgct cccggcgcca ctgctccgcg agctgctcgg
      361 cctccttggt atcgagcgcg acatgcacca ccaccacgtc gctggcgatg cgctcggcgt
      421 actcgatggc ccgcatcgag gcttcgttga gcgcgtcgat cggcacgatc gcggccgact
      481 cgcgcggcca gttggcggcc ccacggtgct cttgcggcgg caggacgagc tgcgccgcca
      541 cgcgctggta gtggcggtgg atggcgagca tggtcgcgat caggatcggc aggagcagca
      601 gcacgatcca ggcgccggtg aggaacttgg tggtgatgat gatgaccgcg acgacaccgg
      661 tcgtcagcgc gcccagcccg ttgatcagca tccccgaatt gcgctggtgc cccttcggct
      721 cccgcttcca ccagcggtag gtcatgctcg cctgtgagag cgtgaaggcc aggaagacgc
      781 cgaccgcgta gagcgggatc agggcgttga cgctggcgcc gaagccgatc accaggatac
      841 tggaggcgat cccgagcgcc aggataccgg tcgagtaggc cagccggtcg ccccgcaggc
      901 ggaactgatg cggcaggaag ccgtcgcgcg ccagaaagaa ggagaggcgc gggaagtcgg
      961 agaagctggt gttggcggcc agcagcagga taccggcggt cgtcgcctgc acgtagtagt
     1021 agaagggcga gcgcccgacg accgtgcgcg tgagctggga gaggatggtt tcgttggcgc
     1081 tcggcaccag gtggaagtgg taggtcagga tggtgatgcc gaggaagagg ctgccgagca
     1141 ccagcgccat cgcggtcagc gtgcggcgcg cgttgcgggc ttcgggcggc tggaaggccg
     1201 gcacgccgtc ggagatggcc tcgaccccgg tcagcgcggt gcagcccgag gagaaagccc
     1261 gcagcaccag gaagatggtc agcgccttgg tcgcgggcgg gatggagtgc gtcaccgggt
     1321 gtggggtgaa gaggtggaac caggccgccg ccagcccgag caggatgacc gcgaacatgc
     1381 cgacgatgaa gagataggtg ggcacggcga agatgctgcc gctctcgcgc acgccgcgca
     1441 ggttggccag cgtgatgaag acgatcgcgc cgacggccag ctccacccgg tagggcgcca
     1501 ggctatcgaa ggccgaggtg atggcggcca cgccggagga gatgctgacc gcgaccgtca
     1561 gggtgtagtc gatcagcagc gaggacgcgg ccagcaggct cggcccggtg ccgaggttgt
     1621 ccttggtgac gatgtaggag ccgccgccct gggggtaggc acggatcgtc tgccggtagg
     1681 aggaggccac gatcgccagc agggcgatga tggcgatgcc gatggggatg acgtactgga
     1741 aggcgacgct gcccgccagc accaggatca gcagcgtctc ctcggtggcg taggcggtgg
     1801 aggagatggc gtcggaggag agcacggcca gcgccttgac cttggtcagg cgctcgtgga
     1861 tctcgtgcgc ggtcgggatc ggggcgccga tcagcacccg gtgcacgcgt cgataggcgc
     1921 ggccgaagga cgacttcgcc gcgctcggct cggcgaccag ataggtcggc gaggcgggcg
     1981 ggcgcagctc cgtcgagcga atgacgcgga tgtggcggtc tcccggccgc cgtccgcgga
     2041 tcgcctcgcg gggccgctcc tctgccggct cgcctggctc gcgcgggggc tcctgctcgt
     2101 gcatccccac cgctcgcttc ccccaacccc ccaggtgaga cgctagagat acttcagcag
     2161 ctcgacccgg ttcccctcgg gatcgcggta gaaggcgacc atcacctcct gcccacccgg
     2221 cagcacgcgg tgctccagcg cggtctcgac cggcgtgccg acctgctcga cgcgggcgca
     2281 gacggcctcg atgttgtcga tgcccagccc gatgtggtcg agcgcgccac gctcgccgat
     2341 ctcctccggc gtgcgccgga ccagctgcag gccgcgcagc cagacgaccg ccggccgctc
     2401 ttccggcccc tggctgcgca cgaagggcag gccgatggtg tcgcggtaga agcggagcgc
     2461 cgcctccaga tcgctcacct tcagtgccgt gtgactgacc gccggattga actccgccgc
     2521 gaagctccct gacatggctc ctctcctccc atcagcggcc actcgccgcc ggacgcatcc
     2581 tatcgcatcc agaggcgagg agtagcctgt accgtggaat tggttggcca gcttttcacg
     2641 gattgtcttg tcctcggccg gccctgtggc ggaggattcg ggctatgatg aaccgtgccc
     2701 ggtgcggatc acgctgatat ggcggcacgc attctgggcg gcgaccggtg agcggcctgt
     2761 gcgtggcgca ggcggcaatg ggtcacaggt caaaggaggc aggcagcatg ttcgagacct
     2821 acatcggcgc gaatggcggc gtcttccggc tgagcggcgc gaaactggag ccgctgggcc
     2881 tggagggcaa gcgcgtcagc gcggtctacg cctggcgcga caacggcagc acggtcgtgc
     2941 tggccggctg ctacggcgac ggtatcttcc gcagccagga cggcggtcag cactggagcg
     3001 aggccaacca ggggctgagc gcaccggtgc tgcgcatgat ccaggcggac ccgacccacc
     3061 ccggcgcgct catctgcggc accgagccgg cgcgcgcctt ccgcagcagc gacggcgggc
     3121 ggagctggca agagttgggc ggcgtcaccg acctggagaa ctaccaggag tggtacctgc
     3181 catactcgcc gcgcgccggc gccctgcgca acttcttctc tcccccgggg caatcggacc
     3241 gactgctcgg cgcgatcgag gtcggcgggc tgatcgacag ccgcgacggc ggcaaaacct
     3301 ggtcctacgt cgacgtcggc cccgaccacg acatccactg ggtcacgggc gacccgcacg
     3361 agcccaaccg gctcttcgcc gcgctcggct atgcccgcat gccctggcag cgcgagctac
     3421 cgcacatgcc gcgcttcggc ggtgtcggtc gctcgcgcga cggcggcgcc acctgggaga
     3481 aactcttgac cgactacacc cgcgccgtgc tggtgctgcc cgagcggccc aacatggtgc
     3541 tggccggacc ctcaccggag gtcgggcgcc agggacgcat cgaggtctcg gtcgatggcg
     3601 gcgaccactg gctgccggcc agcgacggca tcagcgtgcc gatggaggac atggtcgagc
     3661 gcttctacct ggcgccggac ggccacgtct gggcggtgac ctccggcggg cgcgtcttca
     3721 cggccaaccc ggacgactgg cagtggcagg cgccgctgcc gggcgggcag ccggtcgagg
     3781 ccgagtcggt cagcttcgtg gcctagtcgc tcgtcacggc gcagccgtcg accagcgcgg
     3841 cgatggcgtt gcgagccgcc tcgcgcgcct cgccgtagcc ggcgaggcca gcctgcgacg
     3901 cgccgtgcag cggctccgga acgccgcgcg ggcccagttc gagcaactgg agcgtgtagc
     3961 tgggctggag cgaggaccag tcggcgcgga tcacggcctg aaaggtgcgc ccgcggtagg
     4021 tcacctgcag cgtcgaggta cgcactcccg cgatctcgct gacgatcgtc ggcgctggtt
     4081 gcatcatcgt cgtttccttc gggcggagaa gaatggcggc gaggtcactc gccaccgtgc
     4141 catgccgtac ggtcccgcgc cggcgatgag ggtgtcgatg gtgccgtaga catctgctcg
     4201 ctccctctcc tgcgccgctg gcgcggcccg acggtgagag ggagaccttc ggcagcgcag
     4261 ccgtcgtggc ccagaggagc ggccgtagac ctgtcgcttt gcggccccgc ctttcggcgg
     4321 gtgtgccctt gtcgcgatct cccggatcga aacattcttg ccaggttcca gcctagcgcg
     4381 gcacggtcca gatcacgagg gctattcgga ccgtcgggga cgggagtttg ggccgggcgt
     4441 gatggactgg gaccggcgta ccgcggccgg gagtgcggcc cccggcgaac gtgctagagt
     4501 ggccacgcga tcgcgccgca tacggcgccg cgagatgctg gggatagggg agttggggcg
     4561 actcgtcggg atcaaacacc gcggcaggcc cgcgccaccg tcgccggcgc gcgcggactg
     4621 gctcgaccgt ttcccctggg tcttgcttcg cctggcgcgc gccggcagcg gccacgccgt
     4681 cggcaccctc agcgtctggc tctggtggga tcgcttcgcg gcgcactact ggcatatccg
//
LOCUS       DASUBI010000142         5165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1182288, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000142 DASUBI010000000
VERSION     DASUBI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182288"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFU72_02700"
     CDS             complement(<1..519)
                     /locus_tag="VFU72_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422425.1"
                     /translation="MLMARGLTFWKQVRRGEIPRVPIGSFALADQALISGFNFVFTVL
                     LARAFSAATFGTFAVGYVTLLLVNSLQAALVTQPHNVIGPSLTTDEYRAYTSSVLVEQ
                     LLLSATLGVLVLGAGLILSRLALISLAFAAALALAVVAWQAQELLRRVMYTESRFAAA
                     LLNDLVSYGGQLA"
     gene            complement(634..1572)
                     /locus_tag="VFU72_02705"
     CDS             complement(634..1572)
                     /locus_tag="VFU72_02705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422426.1"
                     /translation="MRSAAGLNARKFLKPASFALFRACDRLGVHILPKHYYTPIPDYA
                     WLAANRDVWTPRASLAGVCWDLDQQLEWLATTCRPYYAEVAGLGVYQEITRSQAGPGF
                     GPIESQVLHCFVRAAKPRRIIEIGSGVSTLVMLHAAALNEQGAAPPAEITCIEPYPSE
                     VLKRLHGIRRIQQPAQLVGAELFAQLQPGDLLFIDSSHAVKTGSDVPHVYLNIIPSLA
                     PGVFVHIHDVFLPYLYPRDVLSSYFGWQETALLLALLVNNEHLAVRACLSALGYDRTE
                     QLRAILPDYAPEASIEGLPAPGARGDFPTSTWLQVQ"
     gene            complement(1641..2660)
                     /locus_tag="VFU72_02710"
     CDS             complement(1641..2660)
                     /locus_tag="VFU72_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5422427.1"
                     /translation="MQGRVLVTGAGGFIGHHLVTALRQAGYWVRGVDLKRPEFAPTQA
                     EEFLLLDLREFENCRAAVEGIDEAYNLAADMGGIGYISANHAGIARNNVLINSHMLEA
                     ARLAGVKRFLYSSSACIYPAYRQESADVTPLREEDAYPAMPEEGYGWEKLFSEKLCQY
                     YFQDFGLETRVVRFHNVYGPFGSYQGGREKAPAAICRKVALAQDGDTIQVWGDGRQTR
                     SFMYIDDCVEGLLRLMRSDHHEPLNLGTDRLVTIDELVDIVAGIAGKTLHKRHDLSQP
                     QGVRGRNSDNSRLRAVLGWEPAITLEGGLRATYSWIADQLAPVPVPVQVSRLYAEAAA
                     DDAGR"
     gene            complement(2674..3390)
                     /locus_tag="VFU72_02715"
     CDS             complement(2674..3390)
                     /locus_tag="VFU72_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5422428.1"
                     /translation="MRVDALTYESAGEHILAWARDGQPRSVCVATVHMVMEGHDDPAF
                     QQIVNGADLVTSDGMPLVWGLRLLGCPQARRVYGPDLTLALCEAAADAGIPVGFYGST
                     APVLDSLIVRLSARYPGLPVVYRHSPPFRLLSSEEEAAEIASIRASGCRILFVGLGCP
                     KQERWMAAHRGQLDAVLLGVGAAFDFLGGNKRRAPRRMQALGLEWLFRLATEPRRLWR
                     RYLIHNPRFLALFARQVLAR"
     gene            complement(3444..4226)
                     /locus_tag="VFU72_02720"
     CDS             complement(3444..4226)
                     /locus_tag="VFU72_02720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5422429.1"
                     /translation="MLIPRRQLSDWNPDQGRPAPVASHARSGSGTIIVLDPQQHSGID
                     SRLDLNTLEHRIVEALQSLPATRHDRYALAKRALDITAAGVGLMLLAPFLAIIALLIR
                     LESPGPSFFVCQRVGRDGRTFTMIKFRSMFQGAENLLAFAANKQPGDCRVTRVGRLLR
                     RTSLDEVPQLINVLRGEMSLVGPRPELPEIVARYYEPWQYPRFSVPQGMTGWWQVTGR
                     GAKLMRDHTADDLYYIEHASFWFDLKILALTLRAVLRMDGAF"
     gene            4838..>5165
                     /locus_tag="VFU72_02725"
     CDS             4838..>5165
                     /locus_tag="VFU72_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422430.1"
                     /translation="MDVRQYLDYLREQQGAMVDELGRYVTIESGSREKDGVDRVGREV
                     SKALTELGFSIERIESETCGDHLVARRPGAGRGRLLALIHLDTTCPSGSLADNPFRVE
                     DGRAFGP"
BASE COUNT          920 a         1677 c         1775 g          793 t
ORIGIN      
        1 cgccagttgc ccgccgtagc tcaccagatc gttgagcaat gccgcggcga agcgggactc
       61 ggtgtacatg acgcgccgga gcagctcctg cgcctgccag gcgacgacgg ccagcgcgag
      121 cgccgccgcg aatgccagcg agatcagcgc caggcgcgac aggatcagcc cggccccgag
      181 caccagcacg cccagggttg cgctcagcag gagctgttcg accaggacgg acgaggtata
      241 ggcgcggtat tcatccgtcg tcagactcgg cccgatgacg ttgtggggct gcgtcaccag
      301 cgcggcctgc aagctgttga ccagcagcag cgtgacgtag ccgacggcga acgtgccgaa
      361 ggtcgcggcg ctgaacgcgc gcgccagcag caccgtaaag acgaaattga aaccggagat
      421 cagcgcctga tcggccagcg cgaacgaacc gatcggcacg cgcgggattt ccccgcgccg
      481 cacctgcttc caaaacgtca gccctcgcgc catcagcatg tgcgtcttag ttcctcgcca
      541 gcgccgggtg ccgcgtggcg ggcagccgcg gaggccggga gagggacgac gccccgcgct
      601 tcacctccgg tcgtgccggc cttgggatag ggttcattga acttgcaacc aagtgctggt
      661 cggaaaatcg cctcgcgcgc ccggggccgg cagtccctcg atgctcgctt ccggcgcata
      721 gtcgggcagg atcgctcgga gctgctcggt gcggtcatag cccagagccg acaggcaggc
      781 gcgcaccgcc aggtgctcgt tgttcaccag caaggccagc agcaaggcgg tctcctgcca
      841 gccgaaatag ctagagagca cgtcccgggg atagaggtac ggcaggaaga cgtcgtggat
      901 gtggacgaag acgccggggg ccaggctcgg gatgatgttc agatagacgt gcggcacgtc
      961 cgagccggtc ttcaccgcgt gggacgagtc gatgaacagg agatcacccg gttgcagctg
     1021 ggcgaagagc tcggcgccca ccagttgggc cggctgctga atgcgacgaa tgccgtggag
     1081 acgcttgagc acctcgcttg ggtacggctc gatgcaggtg atctccgcgg gcggcgcggc
     1141 accctgctcg ttgagcgcgg cggcgtggag catgacaagg gtggagacgc cgctgccgat
     1201 ctcgatgata cgcctgggtt tggccgcgcg cacgaagcag tgcagcacct gggattcgat
     1261 cggcccgaac cccggcccag cctggctgcg cgtgatctcc tggtagacgc ccagtccggc
     1321 gacttcggcg tagtagggcc ggcaggtcgt ggccagccat tccagctgtt ggtcgaggtc
     1381 ccagcacacg ccggccagcg atgctcgcgg cgtccagacg tcacggttgg ccgccaacca
     1441 ggcgtagtca gggatgggcg tgtagtaatg cttcggcagg atgtgcacgc cgagacgatc
     1501 gcacgcccgg aagagcgcga acgacgccgg cttcaggaac ttccgggcgt tgaggcccgc
     1561 ggcggagcgc atcgttccaa acccttcaac actggggtag agatcacctc gcgcccggtc
     1621 caggtgacga cgccgacatc ctatcgtccg gcgtcgtccg ccgccgcctc cgcgtacagg
     1681 cgtgacacct gcaccggtac cgggaccggc gcgagctgat ccgcgatcca ggagtacgtc
     1741 gcccgcaggc caccttcgag cgtgatggcg ggctcccagc cgagcaccgc gcgcagccgg
     1801 ctgttgtcgc tgttgcgacc gcgcacgccc tgtggctggc tgagatcgtg ccgcttgtgc
     1861 agcgtcttgc ccgctatgcc ggccacgata tcgaccagtt cgtcgatcgt caccagccga
     1921 tcagtcccaa gattgagcgg ctcgtggtgg tcggagcgca tcaggcgcag caagccctcg
     1981 acgcagtcgt cgatgtacat gaacgagcgc gtctgccggc cgtcgcccca gacctgaatc
     2041 gtgtcgccgt cctgggccag cgccaccttg cgacagatgg ccgcgggggc cttctcgcgg
     2101 ccaccctgat agctgccgaa cggaccgtag acgttgtgga aacgaacgac gcgtgtctcc
     2161 aacccgaagt cctggaagta atactggcag agcttctcgc tgaagagctt ctcccagccg
     2221 tagccctcct cgggcatggc ggggtaggcg tcctcctcgc gcagcggcgt gacgtcggcc
     2281 gactcctgcc ggtaggctgg gtagatgcag gccgaagacg agtagaggaa gcgcttgacc
     2341 ccggccagcc gggcggcctc cagcatgtga ctgttgatca gcacgttgtt gcgggcgatg
     2401 ccggcgtggt tcgcgctgat atagccgatg ccgcccatgt cggccgccag gttgtaggcc
     2461 tcatcgatgc cctcgacggc ggcgcgacag ttctcgaact cgcgcagatc gagcaggagg
     2521 aactcctcgg cctgggtcgg cgcgaactcg gggcgcttca ggtcgacgcc gcgcacccag
     2581 tagccggcct ggcggagcgc ggtcaccaga tgatggccga tgaacccgcc ggcgccagtc
     2641 accaacaccc gtccctgcat cgtcgtaccc ctcctagcgg gcaagcacct gacgcgcgaa
     2701 cagcgccagg aaacgcggat tgtggatcag atagcggcgc cagagccggc gcggctcggt
     2761 cgccaaccgg aacagccact ccagcccaag ggcctgcatg cggcggggag cccggcgctt
     2821 gttcccgccc agaaagtcga acgccgcgcc cacaccgagc agcacggcat cgagctggcc
     2881 ccggtgggcg gccatccagc gctcttgctt ggggcagccc aacccgacga agaggattcg
     2941 gcaaccggac gcgcggatcg acgcgatctc ggccgcctct tcctccgagc tcaagaggcg
     3001 gaacggcgga ctgtgacgat agaccaccgg cagtccggga tagcgagcgg acagccggac
     3061 gatcaagctg tcgagcaccg gcgcggtcga gccgtagaag cctacgggga tgccggcgtc
     3121 ggcggcggcc tcacacagtg ccagggtcag gtcggggccg tagacccggc gtgcctgggg
     3181 gcagcccagc agccgcagcc cccagacgag cggcatgccg tcggaggtca ccaggtcggc
     3241 gccgttgacg atctgctgga aggcgggatc gtcgtggccc tccatcacca tatggacggt
     3301 ggccacacag acgctgcgcg gctggccgtc gcgcgcccag gccaggatgt gctcgccggc
     3361 gctctcgtag gtcaaggcgt cgacgcgcat gccgaggatg gagcggctct cgaagtcccg
     3421 cggcggagac gcgagtgacg cgctcagaac gcaccgtcca tgcgcagcac cgcgcgcagc
     3481 gtgagcgcca ggatcttcag atcgaaccag aaggaggcgt gctcgatgta gtagaggtca
     3541 tcggcggtgt gatcgcgcat cagcttggcg ccgcgcccgg ttacctgcca ccagccggtc
     3601 attccctgtg gcacgctgaa gcgtgggtac tgccacggct cgtagtagcg cgccacgatc
     3661 tccggcagct ctggacgtgg gccgaccagg ctcatctccc cgcgcagcac gttgatgagc
     3721 tgcggcactt catcgaggct ggtacggcgc agcagccggc caacgcgcgt cacgcgacag
     3781 tcgccgggct gcttgttcgc ggcaaacgcg agcaggttct ccgcgccctg gaacatgctg
     3841 cggaacttga tcatcgtgaa ggtgcggcca tcgcgaccga cgcgctggca gacgaagaat
     3901 gacgggcccg gcgactccaa ccggatcaac agggcgatga tggccaggaa cggcgccagg
     3961 agcatcagtc ccaccccggc ggccgtgatg tcgagggcgc gcttggccag cgcgtagcga
     4021 tcgtgacggg tggccgggag cgactgcagc gcctcgacga tgcgatgctc aagcgtgttg
     4081 agatcgagac gggagtcgat gcccgagtgc tgctgcggat cgagtacgat gatggtgccg
     4141 gagccggagc gagcgtgtga ggccaccggc gcgggtcgac cctgatcagg gttccagtcg
     4201 ctcaattgcc gccgtgggat cagcatgctc aacacctctg cgccgcttgg ggcgagactc
     4261 tcaatatggt tgcgagggca gatcgaccgg catcgtcggc cgtgcgcgca accgaagcgg
     4321 gcatagcgtg gcctagaccg acagcccatc gtgcttcgat cattccttgt ccatctagga
     4381 ccatcgcatt cattacccag taccaggata ccaggacaat ttggtacgtc aaggtactga
     4441 agtaccggga accatgtgac ctacaggcaa gtcgaaagcg agggaattcg agcgtgtgac
     4501 acgcgaatca tccgtacgta cggacaccct tcatgcgtga attcgggtgc gagagcctgt
     4561 cggggcgaac ccggcacggt gcgttcgcct gccgccgcga ggacgaggtg cttcgtgtgg
     4621 atagcgaagc gcggtcattc gccgccgcgc ctggcgaatc cagcatcaac ggagactggc
     4681 ggcgcgttct gctctgtgcg acgatacggc agccacggtg agctcaagac gtggtcctct
     4741 ccaggcaagg acgcaccaac ctccgtggct tcctcgcgcg aagacagcag ctcggattcg
     4801 gcgcgtcagc tggccacatt tctggggagg ttcgaccatg gatgtgcggc agtatctcga
     4861 ctacctgcgc gagcagcagg gggccatggt cgatgaactc ggtcgctatg tgaccatcga
     4921 gagcggctcg cgcgagaagg atggcgtgga ccgggtgggc cgggaggtca gcaaagccct
     4981 caccgagctt ggcttcagca tcgaacgtat cgaatcggaa acctgcggcg accacctggt
     5041 ggcccgtcga ccgggcgcgg ggcgcgggcg cctgctggcg ctcatccacc tggacacgac
     5101 ctgtccatcc gggtcgctgg cggacaaccc ttttcgcgtt gaagacggcc gcgcctttgg
     5161 accgg
//
LOCUS       DASUBI010000143         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1102737, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000143 DASUBI010000000
VERSION     DASUBI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102737"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1926)
                     /locus_tag="VFU72_02730"
     CDS             complement(301..1926)
                     /locus_tag="VFU72_02730"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5422431.1"
                     /translation="MRLPSFLPHLKGLHLAEMTVTDQGVTLHLFATRQTAACPACRQR
                     SHRVHSWYDRTIADRPWGGTTVKLRLRVRRFICRAAACPQRIFAEQLPHLVDRYGRRT
                     HQLRDALRQIGLALGGAAGARLAAALGLPVGRTTLLSLIRAAPLPPSPQPTAVGVDEF
                     AWRRGRRFGTIIVDLEHRRPLDLLPDRDADHVAAWLRQQPQITTVARDRSGLYADAAT
                     RGAPGAQQVVDRWHLLHNLGEALEQFLLHKRAVLRDAASALAAAPAEAPADPSPAALP
                     ALPWQQRAEEAGRQRHAAQLARYEAIHQLHSAGADIAHIARTVGVSRETVYRYLRLPG
                     PPARMRLPARAAALDPYLPYLERRWAEGCRNGKRLWRELRAQGFDRSYSGVARFVARL
                     RRAERAGEPTGATRHATTQPPTPRQVAMLCFRHAQQRTPAQQAYLDHLRQADEAVASA
                     YTLTQDFAAMLRERRGERLDDWLAAAEGCPAPALRRFATGLRGDLNAVRAGLTEPWSN
                     GPTEGFVHKLKLLKRQGYGRAGFDLLRQRVLAA"
     gene            2190..>2477
                     /locus_tag="VFU72_02735"
     CDS             2190..>2477
                     /locus_tag="VFU72_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422432.1"
                     /translation="MRVLFTTQPGEGHFTPLVPLAQALLAAGHEVVVACAPSFCPVVA
                     ARGLRAVPMGLDYTLGKFSERWPALRDVPRGQGLTFIMAHIYAGDLAAHALP"
BASE COUNT          366 a          826 c          925 g          360 t
ORIGIN      
        1 cgtacgcttc cttcgcccgt tgatgcatcg ctcttcatcc gcgactggcc gagcgccgtc
       61 cgatccgccc gaccactttc atctcttgcc ccgagtagca ttggcggcgt gatagcctat
      121 gcccaaagaa acaatcggct atcctggtgt acgttcatca acgccagtgt aggcgacgat
      181 acctggtccg tcaaagctca ccatgttgat gtggacgagc acacgcgggg ctactagtca
      241 ctatgctatg agataatcta gccaaacgcc cggctcttgc cgcctttcga tgatcccctt
      301 tcacgcggcg agcacgcgct gtcgcaagag atcgaagccg gcccgcccgt agccctggcg
      361 cttgagcagc ttgagcttat gcacgaagcc ctcggtcggc ccgttgctcc aaggttcggt
      421 gaggcccgcg cgcaccgcat tgagatcgcc gcgcagtccg gtagcgaagc ggcggagcgc
      481 aggggcgggg cagccctccg ccgcagccaa ccagtcgtcc agccgctcac cacgccgctc
      541 gcgcagcatc gccgcgaagt cctgggtcag cgtgtaggcg ctggccaccg cctcgtcggc
      601 ctgccggagg tggtccaggt aggcctgctg cgccggcgtg cgctgctgcg cgtgcctgaa
      661 gcagagcatc gcgacctggc gcggcgtagg cggttgggtc gtcgcgtgcc gggtggcgcc
      721 ggtgggctcg ccagcgcgct cggcacggcg gagccgcgcg acgaagcgcg ccacgccgct
      781 gtaggagcgg tcgaacccct gcgcgcggag ctcgcgccac aggcgcttcc cgttgcggca
      841 cccctcggcc cagcgccgct ccaggtaggg gagatacggg tcgagggccg cagcgcgggc
      901 ggggaggcgc atgcgcgcgg gcggtccggg cagccgcagg tagcgataga ccgtctcgcg
      961 actcacgccg acggtgcgcg cgatgtgcgc gatgtccgcc ccggcgctgt gcagctggtg
     1021 gatcgcctcg tagcgcgcga gctgggcggc gtggcgctgc cgccccgctt cctcggcgcg
     1081 ctgctgccag gggagggccg gcagtgccgc aggggacggg tcggcgggcg cctctgccgg
     1141 ggcggcggcc agggcgctag cggcgtcacg gagcacggcg cgtttgtgga gcaagaactg
     1201 ctcgagcgct tcgccgagat tatggagcag gtgccagcgg tcgacgacct gctgggcacc
     1261 aggcgcgcca cgcgttgccg cgtcggcata gaggccactg cggtcgcgcg cgacggtggt
     1321 gatctgcggc tgctgccgaa gccaggcggc gacgtggtcg gcgtcgcggt cgggcaacag
     1381 gtcgagaggc cgtcggtgct ccaagtcaac gatgatggtg ccgaagcggc gaccacgacg
     1441 ccaggcgaac tcgtccacgc ccacggccgt gggctgcggg ctgggaggca gcggcgcggc
     1501 gcggatgagg ctgaggagcg tcgtccggcc aacgggcagc ccgagggcgg ccgccaggcg
     1561 ggcaccggcc gcgccgccga gcgccagccc gatctgccgc aaggcgtcgc gcaactggtg
     1621 ggtccgccgc ccgtagcgat ccacgaggtg tggcagctgc tcggcgaaga tgcgctgcgg
     1681 gcaagcggcg gcacggcaga tgaagcggcg cacgcgtagc cgcagcttga ccgtcgtgcc
     1741 gccccagggg cggtcggcga tcgtgcggtc gtaccaactg tggacccgat gcgaacgctg
     1801 ccggcacgcg gggcaggcgg cggtctgcct cgtggcgaag aggtggaggg tgacaccttg
     1861 atcggtaacg gtcatctcgg cgaggtgcag gcccttgagg tgcggcaaga acgagggaag
     1921 acgcatacgg tactccaagc gcaagcgact atagccaaac aggaaagcat agcctccccc
     1981 gtccagaacg gcttggagtg atcatcgaaa ggcggcaaga gccgacctgc ccgacacatg
     2041 tttgcactct ggggtggcag tggttgctgg acgagagtgc gtggcgggcg tagagtggtg
     2101 gcacgagaca acgggcgggc gcgagcgtgg ggcgcgcgtc tcgcgggtgc gagcagtgtc
     2161 cggggcattg ctccagaaag ggacgggtca tgcgcgtcct gttcacgact cagcccggcg
     2221 agggtcactt caccccgctg gtcccgctcg cccaggcgct gctcgcggcg gggcacgagg
     2281 tcgtggtggc ctgcgcgccc tccttctgtc cggtggtggc ggcgcgcggc ctgcgggccg
     2341 tcccgatggg cctggactac acgctgggca agttcagcga gcgctggccc gcgctgcgcg
     2401 acgtgccccg cgggcagggc ctcaccttca tcatggcgca catctacgcg ggcgacctgg
     2461 ccgcccacgc cctcccc
//
LOCUS       DASUBI010000144         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_554905, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000144 DASUBI010000000
VERSION     DASUBI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554905"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1221)
                     /locus_tag="VFU72_02740"
     CDS             complement(64..1221)
                     /locus_tag="VFU72_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEU5422433.1"
                     /translation="MSQDGSGIRREDLNGTFAMEQNERIPSTKRDERGKQALEWGLES
                     AASIRDRKIPLFNRSRRGYHHGGQFLDAPFLTDMRELGGQDLAFMGVPLDCGTTNRPG
                     TRYGPQALRRSSGHGYNPEIGVDLGASLDMIDVGDVTVIPANIEKSFDQIALGIGYLH
                     ERGVFPVVLGGDHSIGYPDIRGIAPYIDGNVGIIHFDRHSDLAEYSLDERMHGSPFFH
                     ATNIPNAPATNLVQIGIGGFTGMYDGLRVARDRRATVITLDDVDRYGVERVAEYALEI
                     AWKDAKAVFLSFDIDSVDPAFAPGTGTPEPGGFLPREVLRLLRLVTREGLIGMELVEV
                     SPPYDVSDITALLGARVIKEVLATLVLNGKLGQAPSGAAEEEPAQPSQLHP"
     gene            complement(1344..1649)
                     /locus_tag="VFU72_02745"
     CDS             complement(1344..1649)
                     /locus_tag="VFU72_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422434.1"
                     /translation="MPDEVPARDQLVVELHARDLAASRAVYQRLGFRVTLGKGNYVEL
                     AWGRRLVDARLGSRRLPAAATDRHYPRHGADGRRLLDALPAARPDGLGPRFASRLPM"
     gene            complement(1934..>3000)
                     /locus_tag="VFU72_02750"
     CDS             complement(1934..>3000)
                     /locus_tag="VFU72_02750"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEU5422435.1"
                     /translation="ARLGGDEFTVLLEGIESTARAVEIAERIGAAIATPLRIAGQPAC
                     TTASIGLALSEPGPLGAEEILRHADAALYQAKAQGRARCVIFNPAVNARAREHAELEA
                     ALRHAIDADALSLSFQPAVNLRSGRIVSLEAQTGWIHPLLGWQRNPDLLRLAQEHGLG
                     QPLMRSLLRRACRAAGEWLSLGEATPLSVNVPAPLAHQAGFADLVGAILDEQRLEPAR
                     LRLDITESATLDERDWVEPALHELARRGVRLAIDDFGIGQSSLASLRHLPVDALIIDQ
                     SFVTGLHDVENVAIVRAIVILARTLELQIAAGGIDSAHDLEQARILGCDLGRGDYISP
                     PVAPSEVTGLLRAGAVALPG"
BASE COUNT          435 a         1004 c         1099 g          462 t
ORIGIN      
        1 gataacgcta tagtaggcag cgcgcgccag cttgtcaagg ctggcgcgct cgtgcgttcg
       61 tggctacggg tgcagttggc tcggctgggc cggctcctcc tccgccgcac cgctcggcgc
      121 ctggccgagc ttgccgttga gcaccagcgt cgccagcacc tccttgatga cccgcgctcc
      181 cagcagcgcg gtgatgtcgg agacgtcgta gggcggcgag acctcgacta gctccatgcc
      241 gatcaagccc tcgcgcgtga ccagccgcag caggcgcagc acctcgcgcg gcaggaagcc
      301 gcccggctcg ggcgtgccgg tgcctggggc gaaggcggga tcgaccgagt cgatgtcgaa
      361 gctgaggaag acggccttgg cgtccttcca ggcgatctcc agcgcgtact cggccacacg
      421 ctcgacgccg tagcggtcga catcgtccag cgtgatcacg gtggcgcggc ggtcgcgcgc
      481 cacgcgcagt ccgtcataca tgccggtgaa gccgccgatg ccgatctgga ccagattggt
      541 ggcgggcgcg ttggggatat tggtggcgtg gaagaagggc gagccgtgca tgcgctcgtc
      601 gagcgagtac tccgccaggt cgctgtggcg gtcgaagtgg atgatgccga cgttgccgtc
      661 gatgtagggc gcgatgccgc ggatatcggg gtagccgatc gagtggtcgc cgccgagcac
      721 caccgggaag acgccgcgct cgtggaggta gccgatgccg agcgcgatct ggtcgaagct
      781 cttctcgatg ttagccggga tcacggtcac gtcgccgacg tcgatcatgt cgagcgacgc
      841 cccgaggtcg acgccgatct cggggttgta gccgtgcccg gaggagcgcc gcagcgcctg
      901 cgggccgtag cgggtgccgg ggcggttggt ggtgccgcag tcgagcggca cgcccatgaa
      961 ggccaggtcc tgcccgccca gctcgcgcat gtcggtcagg aagggcgcgt ccaggaactg
     1021 cccgccgtgg tggtagccgc ggcggctgcg gttgaagagc gggatcttgc ggtcgcggat
     1081 ggaggccgcc gactccaggc cccactcgag cgcctgcttg ccgcgctcgt cgcgcttggt
     1141 cgagggaatg cgctcattct gctccatggc aaaggtgcca ttgagatctt cgcgcctgat
     1201 gccgctgccg tcctggctca tcggatgccc ctttccctgg gtagcgccgc gaaggtccgt
     1261 gactgctcgc gcctcggtcg tcaagtggcg gataccgtca tgctagcgtg gttccgccgg
     1321 caggtcaacg ccggaggccg gcgctacatc gggaggcgac tggcgaagcg cgggccgagg
     1381 ccgtcaggcc gggccgccgg cagagcgtcc agtagtcgtc gaccgtcggc accatgacgc
     1441 ggatagtgcc gatcggtcgc ggcggcgggg agacgtcggg atcctagtcg agcatcaacc
     1501 aggcgtcgcc cccatgccag ctcgacgtaa ttccccttgc cgagcgtgac ccggaagccg
     1561 agccgctggt agacggcgcg cgaggcggcg agatcgcgcg catgcagctc gaccaccagc
     1621 tggtcgcggg ccggcacctc gtcgggcatg gcgggctcct ctcgcaccag ggtgctgcgc
     1681 cttgtatggc accgccgtgg agggacaggg gtatgctgcc catcgacgac gttggaatcc
     1741 gctccacgtt ggatccttcg acttcgctca gaatgacagg atacgttggt ggcggacgat
     1801 gacgatcagc gtcgcctacc gcccattttc ctcgtcagcc cgtgactagc cctgtcatcc
     1861 tgagcttgtc gaaggatccg tccccacgcg gacgggggct tttcggagga ctgtaggctc
     1921 gcaagcggcg tcgttacccc ggcagagcaa ccgcgcccgc gcggaggagg ccggtgactt
     1981 cagagggggc gaccggcggg gagatgtagt cgccgcgacc caggtcgcag ccgaggatgc
     2041 gcgcctgctc cagatcgtgc gcggaatcga tgccgcccgc cgcgatctgc agctccagcg
     2101 tgcgcgccag gatcacgatg gcgcgcacga tcgccacgtt ctcgacgtcg tgcagcccgg
     2161 tgacgaacga ctggtcgatg atcagcgcgt cgaccggcag gtgccgcagc gaggccagcg
     2221 acgactggcc gatgccgaag tcgtcgatcg ccaggcgcac cccgcgccgg gccagctcgt
     2281 gcagcgccgg ctcgacccaa tcgcgctcgt ccagcgtggc gctctcggtg atgtccaggc
     2341 gcaggcgtgc cggctccagc cgctgctcat cgaggatcgc gccgacgaga tcggcgaagc
     2401 cggcctggtg agccagcggc gcggggacat tgacgctgag cggcgtcgcc tcccccagcg
     2461 acagccactc gccggctgcg cggcaggcgc ggcgcagcag ggaccgcatc agtggctggc
     2521 cgagcccgtg ctcttgggcc agccgcagca gatcggggtt gcgctgccag ccgagcagcg
     2581 ggtgaatcca gccggtctgc gcctctaggc tgacgatccg cccagagcgc aggttcaccg
     2641 cgggctggaa gctgagcgag agcgcgtcgg cgtcgatggc gtggcgcagc gcggcctcca
     2701 gttcggcgtg ctcgcgtgcc cgggcgttga ccgccggatt gaagatgacg cagcgggcgc
     2761 gaccctgggc cttggcctga tagagcgcgg cgtcggcgtg gcgcaggatc tcctcggcgc
     2821 cgagcggccc cggctcgctc agcgccaggc cgatgctggc ggtggtgcag gccggctggc
     2881 cggcgatccg gagtggcgtc gcgatggccg cgccgatgcg ctcggcgatc tccactgccc
     2941 gcgcggtcga ctcgatcccc tccagcagca cggtgaactc gtcgccgccg agccgcgcca
//
LOCUS       DASUBI010000145         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_380332, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000145 DASUBI010000000
VERSION     DASUBI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380332"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1688
                     /gene="metG"
                     /locus_tag="VFU72_02755"
     CDS             156..1688
                     /gene="metG"
                     /locus_tag="VFU72_02755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5422436.1"
                     /translation="MTTQRFYVTTPIYYANGSPHIGSLYTTVVADFLARYHRLAGDTT
                     CFLTGLDEHGEKIFQTAQVRGTTPQPFVDEIAERFQQTWQTFNISYDIFMRTTNPEHG
                     RIVRTVLEQVYASGDIYFAEYEGRYCVGCERFLTEKELVDGRCPDHRTPPEPRREGNW
                     FFRLERRRPWLRAYIEEHPELIAPERYRREALAMLDEPIGDLSISRPRARVPWGIPLP
                     WDESQVTYVWFDALLSYRSALATPDGAREQRFWPSAHHLIGKDILKPHAIFWPAMLHA
                     LGVPVYRRLLVGGYLLGPDGRKMSKSRGNVVDPFALAARYGVDAVRYYLLREFPYGQD
                     GAVSEVALAERYQADLANTLGNLVNRVRAMLLRYRGGVVPDGQPAAGDHALIAEGTTL
                     VTRVSPLVDELRTHLALEQVIAFAQSLNRYVDEQQPWVLARQPEQAVRLDTVLHNLVA
                     GLSILATLLDPAMPGTMAALRAGLALGPRTLAQTACWQTPTGQTIPPSAPVLFPRVES
                     RA"
     gene            1795..2625
                     /locus_tag="VFU72_02760"
     CDS             1795..2625
                     /locus_tag="VFU72_02760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5422437.1"
                     /translation="MEPIERGAGTLQPVWFTRERAIFRQAEPAPALFVKVYQQADQLE
                     WEVAVMRRAAEAGVPVAPLVAFHCGPPAVLITEQVAGVPLSSAYPLAAEATGRLLRRF
                     HALGAAPAFVDGSVSQQRWSGFIRDWAEREIAATLRRGALAEDEARRLRRHFADLAPI
                     LDQRPCALIHGDCQTEHVLIDPASQRVNALLDFVDARPGDPLVDVAVLTLWDRALTPA
                     VLRGDGGDDPDAPTLLPAYRLLRHLAAANWLVANGAPEHASAHERAVQQALAMQVPRR
                     "
     gene            3209..3427
                     /locus_tag="VFU72_02765"
     CDS             3209..3427
                     /locus_tag="VFU72_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5422438.1"
                     /translation="MTRTFTVIDERDPESGWFIGEVVELPGCYTQASDINSLMANIPE
                     AIKVYLASTEVEDPLPQYVGSFQVAVPA"
     gene            3748..4323
                     /locus_tag="VFU72_02770"
     CDS             3748..4323
                     /locus_tag="VFU72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422439.1"
                     /translation="MTEPVPLIILLGPPATGKTTLGRRLAGELRLPCYSRDDFKERLF
                     DCLGWSDRAWSRRLGMASWELFWQVIATQLAAGQATIAETNFRHPRDSERFRLLAARY
                     PYISLQINCFTRGDVLLERHRARALSSERHPGHVDHLESAQAELLTELSAGRTEPLDL
                     GGQVIELDTTDFAAIDYEQLVVTIRRLLTRV"
     gene            complement(4572..4919)
                     /locus_tag="VFU72_02775"
     CDS             complement(4572..4919)
                     /locus_tag="VFU72_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422440.1"
                     /translation="MSTIPAGEIKRRGISAVDEALKQGPVQVIQRNEPRYVVLSVEQY
                     RELREAYEEITRARLRQALEDVEQGRAQRLTAQEISDGLAALQGFAALRQRLPDAEPL
                     DVVQLVREGRDGL"
     gene            complement(5020..5241)
                     /locus_tag="VFU72_02780"
     CDS             complement(5020..5241)
                     /locus_tag="VFU72_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU5422441.1"
                     /translation="MAQYQARKHDRIIRDPNILLGKPVVRGTRIPVALMLAKLVANPS
                     LDDLFLDFPASPSRTSALASNTPATSSSS"
BASE COUNT          862 a         1909 c         1790 g          895 t
ORIGIN      
        1 cgccgttgcc cacgttagcg ggcggaactg acgacgccac ggtcgtcagt cgaaggtcgc
       61 tgtcgtgagg cagcggcaaa ccagggtggt accgcgagcg cgcccctcgt ccctcgggat
      121 ggtgggcgtc ggtttgctcg cggaggtacg ccccgatgac cacgcaacgc ttctacgtca
      181 cgaccccgat ctactacgcc aacggctcgc cgcatatcgg cagcctctac accaccgtcg
      241 tcgccgattt cctggcacgc tatcaccggc tggcgggcga cacgacctgc tttctcaccg
      301 gcctggacga gcatggcgag aagatttttc agacggccca ggtgcgggga acgacgccgc
      361 agccattcgt cgatgagatc gccgagcgct tccagcagac ctggcagacg ttcaacatct
      421 cctacgacat cttcatgcgt accaccaatc cggagcatgg ccgaatcgtg cgcaccgtac
      481 tcgaacaggt ctacgcctcg ggtgatatct acttcgccga gtacgagggg cgctactgcg
      541 tcggctgcga gcgctttctg accgagaagg agctggtcga cggtaggtgc cccgaccacc
      601 gcacgccgcc ggagccgcgc cgcgagggca actggttctt ccgcctggag cgccgccggc
      661 cctggctgcg cgcctacatt gaggagcacc ccgagctgat cgcgcccgag cgctaccgca
      721 gagaggcgct ggccatgctg gacgagccga tcggcgacct ctcgatctcg cggccgcgcg
      781 cgcgcgtgcc ctggggcatc ccgctcccct gggatgagtc tcaagtaacc tacgtctggt
      841 tcgacgcgct gctcagctac cgctcggcgc tggcgacgcc ggatggcgcg cgcgagcagc
      901 gcttctggcc gtcggcgcac cacctgatcg gcaaggacat cctcaagccg cacgccatct
      961 tctggccggc gatgctgcac gcgctgggcg tgccggtcta ccggcgcctg ctggtcggcg
     1021 gctacctgct ggggccggac ggtcgcaaga tgagcaagtc gcgcggcaac gtggtcgacc
     1081 ccttcgcgct ggccgcgcgc tacggcgtcg acgccgtgcg ctactacctg ctgcgcgagt
     1141 tcccctatgg gcaggacggc gccgtctcgg aggtcgcgct ggccgagcgc taccaggccg
     1201 acctggccaa cacgctgggc aacctggtca accgcgtgcg cgccatgctg ctgcgctacc
     1261 gcggcggcgt cgtgccggac gggcagccgg cggcgggcga ccacgcgctg atcgcggagg
     1321 gcacaacgct ggtcacgcgc gtcagcccgc tcgtcgacga gctgcgcacc cacctggcgc
     1381 tggagcaagt gatcgccttc gcccagtcgc tcaaccgcta tgtcgacgag cagcagccct
     1441 gggtgctggc tcgccagccg gagcaggcgg tccggctcga caccgtgctg cacaacctgg
     1501 tggcgggcct gtcgatcctc gccacgctcc tagatccggc catgccgggc acgatggccg
     1561 cactccgcgc cggcctcgcg ctcggcccgc gcacgttggc gcagacggcg tgctggcaaa
     1621 cgcctaccgg ccagacgatc ccgcccagcg cgccggtgct ctttccgcgt gtggagagcc
     1681 gagcatgatt gaggcggcgg cgagggggga aatcacacgg cgggaagacc gatacgtatg
     1741 agtgacgaga cagcagtccc ggatagcaag cttcgtcagg cggcaactgc cgccttggag
     1801 cccatcgagc gcggcgccgg caccctgcaa ccggtctggt tcacccgcga gcgggcgatc
     1861 ttccgccagg ccgagccggc gccggccctc ttcgtcaagg tctaccagca ggccgatcaa
     1921 ctggagtggg aggtggccgt gatgcgccgg gcggcggagg cgggcgtccc ggtcgcgccg
     1981 ctggtggcct ttcactgcgg cccgccggcg gtgctcatca ccgagcaggt cgcgggcgtg
     2041 ccgctgtcgt cggcctatcc gctggcggcg gaggccactg gtcgcctcct gcgccgcttc
     2101 cacgccctcg gcgccgcgcc ggcattcgtg gatggctctg tcagtcaaca gcgctggtcc
     2161 ggcttcatcc gcgactgggc cgagcgtgag atcgccgcta ccctgcggcg cggggcgctc
     2221 gcggaggacg aggcgcggcg gctgcggcgg cacttcgccg atctggctcc catcctcgac
     2281 cagcggccct gcgcgctcat ccacggcgac tgccagacgg agcacgtgct gatcgacccc
     2341 gccagccagc gggtcaacgc gctgctcgat ttcgtcgacg ctcggcccgg tgacccgctg
     2401 gtcgatgtcg cggtgctgac cctctgggac cgcgccctga ccccggccgt cctgcgcggc
     2461 gacggcggcg acgaccccga cgcgcccacc ctgctccccg cgtaccgcct cctccgccac
     2521 ctggccgcgg ccaactggct cgtcgccaac ggcgctccgg agcacgcgtc tgcgcatgag
     2581 cgcgcggtgc aacaggcgct tgcgatgcag gttccgcgtc gatgagttcc ggcagcaata
     2641 actacccacc ctgtgctggc gcagccgtcg acgccgtatg gaagcgagtc caggtctggg
     2701 aggatgcgct ttggcggctg gtcactcgtc tccggggccc gattcccggc tacgcctacc
     2761 tgatcccgaa gcggcacatt gctttcatca ccgatttcga cggtgaggag agccgtacac
     2821 tgggcgaggt gctggcgcgg ctgacggcaa cgctgaggcg accggcgccg cactcatcca
     2881 cgttcacatc ttcggcgggg gcgccgccca tgcacccatc cacctggcgc ccgaccgggc
     2941 cggcgatgcc ctcaacctct cgcggatccg cggcacggtc agctacgagc ccttgccgag
     3001 cggcggccgc atcatgagcc acggatttcc gatgccggag gagcaagagc accgcgaggt
     3061 cgctgagaac gttcgccggg tgctgcaagg tgcgtgacgt gaggtggaat gctcaacgac
     3121 gaatgcgctg cttcctaccc ctgacaccat cgctgcggct acactagcgc gagcggcggc
     3181 gcgcggtgca gtaggcgaag aggcaatgat gacccgtaca ttcacagtca tcgacgaacg
     3241 cgatcctgag tccggctggt tcatcggaga ggtggtggaa ctgccggggt gctataccca
     3301 ggccagcgat atcaactcct tgatggccaa catcccggag gccatcaagg tctatcttgc
     3361 ttccaccgag gttgaggatc ctcttcccca gtacgtcggt tcgttccagg tagccgtgcc
     3421 cgcgtgagca aactccggat tttgccctac cgagatctga aaagcgttgt cgaagcctgc
     3481 ggcttcgtct gcgtctgggt tcgccgcgag ggaagccaca acgcgtttcg gcacccggat
     3541 ggccgagttc ccgtcgtgcc cgatcacggt tccgatccga ttgtccggcc attgctccgc
     3601 aagatcctgc gtgatctcaa cctatcagtc gatgagtgca acaacctact gaaccgttag
     3661 gccgcgccgt gctgcttcct tcggcatctg cgcgagcgtg actcgcacgc gttgccgaag
     3721 gacgcgtgag acgagatggg gcgctcgatg acggagccgg tcccgctgat catcctgctc
     3781 gggccgccag ctaccggcaa gaccacgctc ggccggcgcc tggccggcga gctgcgcctg
     3841 ccctgctaca gccgcgacga cttcaaggag cggctcttcg actgtctcgg ctggagcgac
     3901 cgcgcctggt cgcggcggct cgggatggcc agctgggagc tcttctggca ggtgatcgcc
     3961 acgcagctcg ccgccgggca ggccaccatc gccgagacca acttccgcca tccccgcgat
     4021 agcgagcgct tccggctgct ggcggcgcgt tacccctaca tctcgctgca gatcaactgc
     4081 ttcacccgag gcgatgtcct cctcgagcgc caccgcgcac gcgcgctgtc gagcgagcgc
     4141 caccccggcc acgtcgacca cctggagtcg gcgcaggccg agctcctgac cgaactctcg
     4201 gccgggcgca ccgagccgct ggatctcggc ggccaggtca tcgagctcga caccaccgac
     4261 ttcgccgcca tcgactacga acagcttgtc gtcacgatcc gccggctgct gacgagggtg
     4321 tagcagcttg gtgaattgat ctggcaattg ccgacgaccg cgcggggttg acggaggttg
     4381 ccgaattgat ccgggacctg ggtggccgcc ggcgcgccgg gctaaagccg cagcgctaac
     4441 tccacgaagc ccactgaagg ggctgaggat tgaccgtgag tagaccagag tatgtcgtca
     4501 agtgtcatag gccacgcacg aggagacgaa gccagctact agccggctaa ggagaaaccg
     4561 gctagcgctc gctacagtcc gtcccgtccc tcgcgcacga gttgcaccac atcgagcggc
     4621 tcggcatccg gcagccgctg gcgcagcgcg gcaaaccctt ggagcgcggc gagcccgtcg
     4681 ctgatctcct gtgcggtcag tcgctgtgct cgcccctgct ctacgtcctc caacgcctga
     4741 cggaggcgcg cccgtgtgat ctcctcgtag gcctcacgta gttcccggta ctgctccacg
     4801 ctcagcacca cgtagcgtgg ctcattacgt tggatgacct gcaccggccc ctgcttcagc
     4861 gcctcatcga ccgcgctgat ccctcgccgc ttgatctctc ccgcaggaat cgtgctcatt
     4921 ttagtactcc tcacagtact tcactgagta tcgtagcgag taccttagtt ggagtcaagc
     4981 ggcgtgcgag tgacgctcag ccgacacgaa tgatgctcgt cacgagctcg acgaggtcgc
     5041 gggcgtattc gaggcaagcg cggatgtccg cgacggtgag gcggggaagt cgaggaaaag
     5101 gtcgtcgagg ctcgggtttg ccaccagctt ggcgagcatc agcgccacgg ggatacgggt
     5161 gccgcgcacg accggcttgc cgagcaggat attggggtcg cggatgatgc gatcgtgctt
     5221 tcgagcctgg tactgcgcca tcgtgatcct tgaacctctg caactagtct cgctgccgga
     5281 gacttaccaa ctcctacccc agcgtcactc tcataatcgc cgccgaaccg agagcgacga
     5341 tcaccaccga cagggcgagc agcccccgct ggcggcgggc tcgggcggcg ggggctggcg
     5401 tgtagtcggc gatggcgacc cagaggccgg cggcgccgtg ggccaccgcc gccacc
//
LOCUS       DASUBI010000146         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_78223, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000146 DASUBI010000000
VERSION     DASUBI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78223"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..3154
                     /locus_tag="VFU72_02785"
     CDS             227..3154
                     /locus_tag="VFU72_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422442.1"
                     /translation="MSGEPETGDKARSAEPRVALRTVLRLCFAPQQADALAARARESE
                     SDPAKCLAPTVRLARLNALGGRWPGRSGSQYDGDPPPLRPRSGLPASAALALALHLGL
                     ALDGEVLARLLGRTPAEIGQALRQARQAVEAEGAAPCPEFAAALGRQRDPSLPRSQQL
                     ALMRHLSGCERCRTALERDRALDERLLARLWELECEAPEAPAARARLAPLLLGPALRL
                     GAILLLVLAVAVMAAGAGRRLLARGGEPVPLLAPEPANRSLTGWLLEKSSGGVQATNI
                     VTGERRALIPEQGSSSVSAILSPDQRRLAVLTFAGSERDVVGDLRVYQLNGSLVREWR
                     DIGQGGQSYLEGWLNDDLLLVGRLTTGNTVQLLALSLSSGAQSVLLGQGVRGPAALSP
                     DGHYLALTMVSREGPIDLELRPVVSGQLGAPLVTHRLPSSFLNSGPLWTTDSRTVLYT
                     LAATENAGPSMESLALDGTWTRRLTRPGTGEIRLLGLASDGRGALYAERASQPGGASS
                     PSSVVWEILLDGGQPVHFLASDSSFSQAVRAPGGTALALQVTAYSYPPQLRSDGSLTT
                     LLVGSLSNSVIFMSNTVTLALGPDGKLIGTLLDEFAPGELLAWLPQDALPERATPAPH
                     VGGAFGVPEQVKVIGATAQLDAASRPSPGGGWVLATGVGFPFALAVPVHGQPLAQVAG
                     PPLDASWLPGGPGTIGVLSPAGSGKGPDRVSIYPMSGDMQMLAQANFNPADLTGAPGQ
                     HYRQPLLSPNGLRYSVFVVEERQVALWVGGEDTLARSVIDWTPPSDARVDPPLLAEWI
                     GNDALLVAVPQDWSGGLPTAVALERITLAPDGSASVEPLLRWRTRGSERGIVARELAL
                     SPDQTHLAIRMRRFGGYDPAHDYADALLVAPSSDLTQSLELARGAGGDGLSWAPDGTE
                     LVAALRGHLLVLAADGNHVDQLATGEGPVAHPLWVRPGEIWYSSGSGDDAKIMRVDRP
                     GAG"
     gene            complement(3337..>3547)
                     /locus_tag="VFU72_02790"
     CDS             complement(3337..>3547)
                     /locus_tag="VFU72_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422443.1"
                     /translation="FTVPFDNNQAERDLRMVKVQQKIAGCFRTDSGAEQFCRIRGYLS
                     TARKQGHQPLAALEAVFRGQPLVLT"
BASE COUNT          495 a         1224 c         1291 g          537 t
ORIGIN      
        1 gcgcgacccc gaactccagc tccagcttga gccgcaccag cgccgggaaa tccatcgcct
       61 gtcgcctcca tcgtgagctg gcatcgacca ggccattatc gcgcgtccag agcgggaacc
      121 gaacgaccgc gggtggtgta gtatgcatgc gaaccctgcg atgcggtacc gcatgacttc
      181 tcggacgacg ccgtgatccg gcctgggacg gcgatgggag cgggcggtga gcggtgagcc
      241 ggagacgggt gacaaggcca ggagcgccga accgcgcgtt gcgctccgca cggtgctgcg
      301 gctctgcttc gcgccgcagc aggccgacgc gctggctgcc cgcgcccgtg aaagcgagtc
      361 ggaccccgcc aagtgcctgg ctccgaccgt gcggctggcg cggctgaacg cgctcggcgg
      421 ccgctggccg ggacgctcgg gtagtcagta cgacggtgat ccgccgccgc tgcggccgcg
      481 ctccggcttg ccggcttccg ccgcgctggc gctcgctctt catctcgggc tcgccctgga
      541 tggtgaggtg ctggcgcggc tcctcggccg cacgccggcg gagatcgggc aggcgctgcg
      601 ccaggcacgg caagccgtcg aggcggaggg cgcggcgccc tgccccgagt tcgccgctgc
      661 cctgggccgc cagcgagacc cgtcgctgcc gcgcagccag caactggcgc tgatgcgaca
      721 cctctccggc tgcgagcgct gccggacggc gctggagcgg gaccgcgcgc tcgacgagcg
      781 gttgctggcg cgactgtggg agctggaatg cgaggcgccg gaagcgccag ccgcccgggc
      841 gcggctggcc ccgctgctgc tcggtccagc gctccggctg ggggcgatcc tgctgctggt
      901 gctggcggtc gccgtaatgg cagccggcgc gggccgccgg ctcctggcac gcggcgggga
      961 gccggtgccg ctgctggcgc cggagcccgc caatcgctcg ctgactggat ggctgctgga
     1021 gaagtcgagc ggcggggtgc aggcgaccaa catcgtcacc ggcgagcggc gcgcgctcat
     1081 tcccgagcaa ggcagctctt cggttagcgc catcctctcc cccgatcagc ggcgcctcgc
     1141 agtgctgacc tttgccggca gcgagcgaga cgtcgtcggt gatctccgcg tctaccagct
     1201 caatggttcc ctcgtgcgtg aatggcgcga catcggccag ggcgggcaga gctacttgga
     1261 gggctggctc aacgacgatc tgcttttggt cggtcgattg accacgggaa acaccgtgca
     1321 gctgctggcg ttgagcctgt cgagcggcgc tcagagcgtg ctcctggggc aaggggtgcg
     1381 cgggccggct gccctttcgc ccgacggaca ctatctcgcg ctcacgatgg tcagccgtga
     1441 gggcccgata gatttggagc tccggcccgt cgtgagcggc caactcggtg ctccgctggt
     1501 gacgcatcgc ttgccgagca gcttcctcaa ctccggcccg ctctggacga ccgatagccg
     1561 gacggtcctc tacaccctcg cggcgaccga gaacgccggc ccgagtatgg agagcctggc
     1621 gctggacggg acgtggacgc ggcggctgac ccgccccggc accggcgaga ttcgcctgct
     1681 cggtctcgct tccgacggtc gcggcgcgct ctacgccgag cgcgcgagcc agccgggcgg
     1741 tgccagcagc ccgtcgtcgg tcgtctggga gatcctgctc gacggcggcc agccggttca
     1801 tttcctggcc agcgactcca gcttcagtca ggcggtccgc gcgcctggcg gcacggcgct
     1861 cgccctgcag gtcacggcct acagctaccc gccgcagctc agatcggatg gctcgctgac
     1921 cacgctactg gtcggctcac tcagcaacag tgtcatcttc atgagcaaca ctgttacgct
     1981 ggcacttggc cccgacggca agctgatcgg tacgctgctc gacgagttcg cgcccggcga
     2041 gctgctcgcc tggctgccgc aggacgccct gccggagcgg gctacacccg cgccgcacgt
     2101 cggcggcgcg ttcggtgtgc cggagcaggt caaggtgatc ggcgcgacgg cgcagctcga
     2161 cgccgccagc cggcccagcc ccgggggcgg ctgggtgctc gctacgggcg tcggtttccc
     2221 gttcgcgctg gcggtgccgg tgcacggtca gccgctggcg caggtcgcgg gaccgccgct
     2281 cgacgcgagt tggctgccgg gcggcccggg cacaatcggc gtcctatcgc ctgctggaag
     2341 tggtaaagga ccagaccgcg tcagtatcta ccccatgagc ggcgatatgc agatgctggc
     2401 ccaggccaac ttcaacccgg ccgacctgac gggagcgccc ggccagcact accgccagcc
     2461 gctgctgtcg cccaacggac tgcgctatag cgtctttgtg gtggaagagc gccaggtagc
     2521 gctctgggtc ggcggcgagg atacgctcgc caggagcgtg atcgactgga cgccgccgtc
     2581 cgatgccagg gtcgacccgc cgctgctggc agagtggatc ggcaacgatg cgctgctggt
     2641 cgcggtgccg caggactggt ccggcggctt gcccaccgcc gtcgcgctgg agcggatcac
     2701 gctcgcgccg gatggctcgg ccagcgtcga gccgctgctg cgctggcgca cgcgcggcag
     2761 cgagcgcggc atcgtcgctc gcgaactggc gctcagcccc gaccagacgc acctggccat
     2821 ccgaatgaga cgcttcggcg gttatgaccc ggcacacgac tacgccgacg cactgctggt
     2881 cgccccgtcc agcgatctga cgcagtcgtt ggagctggcg cgcggcgcgg gcggcgacgg
     2941 gctgagctgg gcgccggacg gcaccgagct ggtggcagcc ctgcgcggcc acctgctggt
     3001 actggcggcc gacggcaacc acgtcgatca actcgcgacc ggtgaggggc cggttgccca
     3061 cccgctctgg gtgcggccgg gcgagatctg gtacagcagc ggcagcggcg acgacgcgaa
     3121 gatcatgcgc gtcgaccggc cgggcgccgg gtgagcgcgc caggcgttgc ctatcgaaaa
     3181 cgttagacgc catcgaggac ggatccttcg actgcgctca ggatgacagg gtaagtaagg
     3241 ggctgacgag gaggaggggt cttatgccat gcacctcttc gtccggtagg cccgaccctc
     3301 tccctgtcat cctgagcgca gtcgtaacta ttcaggtcag gtgaggacga ggggctgccc
     3361 gcggaagacg gcttccaagg cggcgagggg ctggtgcccc tgcttgcggg ccgtcgagag
     3421 gtagccccgg atgcggcaga actgctcggc cccgctgtcc gtgcggaagc agccggcgat
     3481 cttctgctgc accttgacca tgcgcagatc gcgctcggcc tggttgttgt cgaagggcac
     3541 ggtgaag
//
LOCUS       DASUBI010000147         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_363773, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000147 DASUBI010000000
VERSION     DASUBI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363773"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFU72_02795"
     CDS             complement(<1..636)
                     /locus_tag="VFU72_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422444.1"
                     /translation="MRSRIPEFDRQAESFDARAGLGPEAGAEIARAIVQLTGLGPDDL
                     VLEIGAGTGELGQHLARQAARYLGLDLSRPLLSVFSSKLPAVPLAVADCEQPWPLPDG
                     SVRAIVASRVAHRLRPEPVTGEAMRVLRPGGVLLIGQIQRDPASLMEQLRAERRRIAS
                     GGRGGGQERRREARSLIESLVAAGASELSPSVAARWERRRSPREIIAGWEGL"
     gene            complement(752..1699)
                     /locus_tag="VFU72_02800"
     CDS             complement(752..1699)
                     /locus_tag="VFU72_02800"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEU5422445.1"
                     /translation="MTSVVVVGSVNTDLVARVRRFPAAGETVFAESFDTFGGGKGANQ
                     AVAAARLGAPTRLVGAVGTDAESQARLDALGADGVDCAGVRRFSGHGGMALIEVENLS
                     GQNRITVVSGANALVDAALVAEALAPALAPGDIVCCQLELPLAAVECALRLARDRCAT
                     TVLNAAPAQAGLEPLLALCDVLVVNEIEAGQLLGVTPLAPGDAEEGAAGLRRFGPPAV
                     IVTLGPAGAFLLDSSGAVRVPAPMVSVVDTTGAGDALVGAICAMLARGASLAEALRAG
                     VAAGSFAVLRAGAQPSFPTAVELDQWQRLLARSSPGEPG"
     gene            complement(1692..>4119)
                     /locus_tag="VFU72_02805"
     CDS             complement(1692..>4119)
                     /locus_tag="VFU72_02805"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEU5422446.1"
                     /translation="QGQRAEDLYLFVLLMGALVWIMAYTSVWLVFRSRSVWLALLFSG
                     IVVFTNLGYSLKVPTTLLILYLFAAILLLMRFSLAQREERWRRSGVPFPDTLAWRGLW
                     VASYLALGVLIFGWVFPVAPQSQSLVTAWQRIDGPWVQVRDSLNGWFSGLRGPNAGGG
                     PGGFAAFSDQFAVGGPLRLSTTPVVLVKGGNSQYLIAHTYSTFTGRSWTSGDDTLASE
                     PLVRLDANQAYPLPSTSAKDRGNTSFEVQILQPRGTVVYTADELGSVTVPSQLQLSWY
                     YYQNQSLDVTTATEANTPPTLWPLVQLLKDADFAPVERGGGVFGLPPDLQVPRERGIT
                     PTASPAPEAAAPGEGTPTPEGSPTPAASATPAASPTPNDFGRLPLVGSLPNVTPPPGR
                     SRVAVPENWNDIHSAMEELTARHIDTQVLLSPTFAVQTLRFTGNLPVYADLEAVFSQS
                     GVRTGDSYTVSSLPSQATVDTLRQASTAYPVEIASRYLQLPTVTPRVSALAHEIGDGQ
                     ANAYDKASAIQNFLRTNYKYNENVNAPPRDDDIVDYFLFESKQGYCTYYSTSMVEMLR
                     ILGIPAREVVGFYPASFDQNAGGFLYRDLNSHAWVEAYFPGYGWIPFEPTAARAEIAR
                     YVPPAPSSPSSSTSGSSQLPPNADGRLQELNELGGGGFGGPVVTQHHTSPYEWALRAL
                     AVLALLALVVLSYLWLRGLRGLSPSTQLFTKLQRGASWGGIRADPALTPYEYAGTVGK
                     RVPGARTHASLLADLYVKERYGAYPVASDELSRARHAWLRLRSLLLRHLLLDQWRPSV
                     RARVLDEADD"
BASE COUNT          697 a         1471 c         1391 g          560 t
ORIGIN      
        1 gaggccctcc caaccggcga taatctcgcg cggcgagcgc cggcgctccc aacgtgccgc
       61 cacgctcggt gacagctcgg acgcgcccgc ggccaccagc gactcgatca agcttcgcgc
      121 ctcgcgccgc cgttcctggc cgccgccccg cccgccgctc gcgatccggc gtcgctcagc
      181 gcgcaattgc tccatcagac tcgccgggtc acgctggatc tgcccgatca gcagcacccc
      241 gcccggccgc agcacccgca tcgcctcacc ggtgacgggc tcgggacgca gccggtgcgc
      301 tacgcgcgac gccacgatgg cgcgcacgga gccatccggc agaggccagg gctgctcgca
      361 gtcggcgacc gccagcggca cagcgggcag tttggagctg aagacggaca gcagggggcg
      421 cgagagatcg agaccaagat aacgcgccgc ttgccgtgcc aggtgctgcc ccagttcgcc
      481 ggtaccggcg ccaatctcca gcaccaggtc atccggcccg agtccagtca gctgaacaat
      541 cgcgcgcgcg atctccgcgc cggcttcagg gcccagcccg gcgcgcgcgt cgaagctctc
      601 ggcctgccga tcgaactccg gtatccgcga cctcaccaaa tcgtcctcac aacagcgcac
      661 ccgtcacgcc ccacgcggtg acgggaagcg ggcacttggc attcctcatc cgttggacct
      721 gaagggaccg tagcacgcag cgcggcccgc gtcaaccggg ttctccgggc gaggaccgcg
      781 ccagcagacg ctgccactgg tcaagctcga cggccgtggg gaatgacggc tgcgcgcccg
      841 cgcgcagcac ggcgaacgag cccgccgcga ccccggcgcg cagcgcctcc gccagtgacg
      901 ccccacgcgc cagcatggcg cagatggcgc cgaccaacgc gtcgccggcg ccggtggtat
      961 ccaccaccga caccatcggc gcgggcaccc tcactgcccc tgagctatcg agcaggaagg
     1021 cgccagccgg gccaagcgtg acgatcaccg cgggcggacc gaagcggcgc aggccggcag
     1081 ccccttcttc agcgtcccca ggcgcgagcg gcgtgacgcc gaggagctgg ccagcctcga
     1141 tctcgttgac caccagtacg tcgcacagcg ccagcaatgg ctccagcccc gcctgcgccg
     1201 gcgcggcgtt cagcacggtc gtggcgcacc gatcccgtgc cagccgcaac gcgcactcta
     1261 ccgccgccaa cggtagctcg agctgacagc agacgatgtc ccccggcgcc agtgccggcg
     1321 cgagcgcctc ggccaccagg gcggcgtcga ccagcgcgtt ggcgccggag accaccgtta
     1381 tccgattttg cccgctgaga ttctcgacct cgatcaacgc catgccgccg tggccggaga
     1441 agcggcgcac gcccgcacag tcgacgccat cggcgcccag cgcgtcgagc cgggcctggc
     1501 tctcggcatc cgtgccgaca gcgcccacca gacgggtcgg tgcacccaac cgggcggcgg
     1561 ccacggcctg attggcgccc ttgcccccgc cgaaggtgtc gaacgactct gcgaagacgg
     1621 tttcgcccgc cgccgggaag cgccgcaccc gggccaccag atcggtgttg acgctcccga
     1681 cgacgacgac gctagtcatc agcctcgtcg agcacccgcg cccgtaccga tggccgccac
     1741 tgatcgagca gcagatgccg caagagcagc gagcgtagcc gcagccaggc gtgtcgagcg
     1801 cggctcagct cgtcactggc caccgggtag gcgccgtagc gctctttgac gtacagatcg
     1861 gccagcaggc tggcgtgggt gcgtgccccc ggcacacgct tgccgacggt tcccgcgtat
     1921 tcatacggcg tgagcgccgg atcggcgcgt atcccgcccc agctcgcgcc ccgctgcagc
     1981 ttcgtgaaga gctgcgtcga cgggctgaga ccgcgcagcc cgcgcagcca gaggtaggag
     2041 agaaccacca gcgccagcag cgccagcacc gccagcgccc gcagggccca ctcgtagggg
     2101 ctggtatggt gctgcgttac gaccgggccg ccgaagccgc cgccgcccag ctcgttcagc
     2161 tcctgcaggc gtccgtcggc gttcggcggc aactggctgg agccggacgt tgagctggag
     2221 gggctggacg gcgcgggcgg cacgtagcgc gcgatctcgg cccgcgcggc ggtcggctcg
     2281 aaggggatcc agccgtagcc ggggaagtag gcctcgaccc aggcgtgcga attcaggtcg
     2341 cggtagagaa agccgccggc attctgatcg aagctggctg ggtagaaacc gaccacctcg
     2401 cgcgccggga tacccaggat gcggagcatc tcgaccatcg acgtcgagta gtaggtgcaa
     2461 tacccctgct tgctctcgaa gaggaagtag tcgacgatat cgtcgtcacg cggcggcgcg
     2521 ttgacgttct cgttgtactt gtagttcgtg cgtaggaagt tctggatggc gctcgccttg
     2581 tcgtaggcgt tcgcctgacc gtcgccgatc tcatgcgcca gcgcgctgac acgcggggtc
     2641 acggtgggga gctgcaggta acggctggca atctcgaccg ggtacgccgt gctcgcctgg
     2701 cgcagcgtgt cgaccgttgc ctgcgagggg agcgacgaca cggtgtaaga atcccccgtg
     2761 cgcaccccgc tctgcgagaa gaccgcctcg aggtcggcgt agaccggcaa gttgccggtg
     2821 aagcgcagcg tctgcacggc gaaggtcggg ctcagcagca cctgagtgtc gatatgcctg
     2881 gccgtcagtt cctccatggc cgagtggatg tcgttccagt tttcgggaac cgccacacgg
     2941 ctgcgccccg gcggcggagt gacgttgggc agcgaaccga ccaggggcag cctgccgaaa
     3001 tcgttggggg tcggagaggc cgcgggggtc gcggaagcgg ccggcgtcgg ggaaccttca
     3061 ggcgtcgggg tgccctctcc tggcgccgca gcctcggggg caggcgatgc tgtcggcgta
     3121 atcccccgct cacgcggcac ctgcaagtcc ggcggcagcc caaagacacc gccgcctcgc
     3181 tcgaccggag cgaaatcagc gtccttcaaa agctgcacca gcggccagag tgtgggcggt
     3241 gtgttcgcct cggtagccgt cgtcacgtcg agcgactgat tctgatagta gtaccaggag
     3301 agctgcaact ggctgggcac cgtcaccgag cccagctcat cggcggtgta gaccaccgtg
     3361 ccgcgcggct ggaggatctg cacctcgaag ctggtattgc cgcggtcctt ggccgacgtg
     3421 ctgggcagcg gataggcctg attcgcgtcg agccgcacga gcggctcgct ggccagcgta
     3481 tcgtcgcccg acgtccagct tcgcccggtg aacgtgctat aggtgtgcgc gatcaagtat
     3541 tgcgagttgc cgcccttgac cagcaccacc ggggtcgtgc tcaggcgcaa cggtccgccg
     3601 accgcgaact gatcgctgaa cgcggcgaaa ccgcctggcc cgccgccggc gttcgggccg
     3661 cgcaggccac tgaaccagcc gttgagcgaa tcgcgcacct gcacccaggg gccgtcgatc
     3721 cgttgccagg cggtgaccag tgactggctc tgcggcgcca ccgggaagac ccagccgaag
     3781 atcagcacgc ccagcgccag gtagctcgcc acccataacc cgcgccaggc cagcgtatcg
     3841 ggaaaaggta cgccgctgcg ccgccagcgc tcctcgcgct gcgccaggct aaagcgcatc
     3901 agcagcaaga tcgcggcgaa gaggtagagg atcagcaacg tcgtgggcac tttcagcgag
     3961 tagcccaagt tggtgaaaac cacgatcccg gagaagagca atgcgagcca gaccgagcgc
     4021 gagcggaaga ccagccagac cgaggtgtag gccatgatcc acaccagcgc gcccatcagc
     4081 agcacgaaga ggtagagatc ttcggcacgc tgcccctgg
//
LOCUS       DASUBI010000148         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_690658, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000148 DASUBI010000000
VERSION     DASUBI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690658"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFU72_02810"
     CDS             complement(<1..688)
                     /locus_tag="VFU72_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5422447.1"
                     /translation="MFQRFFGSSNDRKVRTMLGRVGRINDMEPQIQALSNEQLRAKTE
                     EFRARFANGETLDDLLEEAFAVVREAARRTLGQRHYDVQLVGGMVLHQGGIAEMRTGE
                     GKTLVATAPVYLNALAGKGVHLITVNDYLAQRDAEWMGQIYGFLGMSVGVIVHGLSQG
                     QRQASYNADITYGTNNEFGFDYLRDNLVYDASEMVQRGHNFAIVDEVDSILIDEARTP
                     LIISGPTEDRS"
     gene            complement(857..2026)
                     /locus_tag="VFU72_02815"
     CDS             complement(857..2026)
                     /locus_tag="VFU72_02815"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5422448.1"
                     /translation="MREAVIVSYARTGLAKSARGGFNMTPVMSMAAHAIKHAVDRAGV
                     EKEAVEDVFLGNVAHPNGDPARQAALLAGLPVTTAGVTINRFCSSGLNAIAAAANYVK
                     NDGAEVVVAGGVESISMPPGRGSAENNDPTLTQMYPAFHMPMIDTADVVAARYKISRE
                     YQDEYSLESQRRMAAAQQAGKFRDEIVPMKTKMKQVNKETGEEKIVDYEVNRDECNRP
                     DTTLEGLAKLQPVKGEGKFITAGNASQLSDGAAAVVIMEAKEAERRGLEPMGVFKGWA
                     VAGCEPDEMGIGPVFAVPRLLERHGLKVDDIELWELNEAFASQCLYCRDTLGVDPEIY
                     NVNGGSIAIGHPYGMTGARLTGHILQEGKRRGVKNVVVSMCIGTGMGGAGLFEVL"
     gene            2114..>2445
                     /locus_tag="VFU72_02820"
     CDS             2114..>2445
                     /locus_tag="VFU72_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422449.1"
                     /translation="MARDGARASAISIPPADARKTPPHQDNSDGGPVMSRKPRTAKAL
                     EVAAKPVEVVGQALERAFDPLTSTLKRAALKRADKQARQFAQATTAAPTNKAERTDLM
                     VSPLAVPFP"
BASE COUNT          368 a          842 c          810 g          425 t
ORIGIN      
        1 ccgagcggtc ctcggtgggc cccgagatga tcagcggcgt gcgcgcctcg tcgatcagga
       61 tcgagtccac ctcgtcgacg atcgcgaaat tgtggccccg ctggaccatc tcgctggcgt
      121 cgtagaccag gttgtcgcgc aggtagtcga agccgaactc gttgttggtc ccgtaggtga
      181 tgtcggcgtt gtaggaggcc tgccgctgcc cctgcgagag gccgtgcacg atcaccccga
      241 cgctcatgcc gaggaagccg tagatctggc ccatccattc ggcgtcgcgc tgcgccaggt
      301 agtcgttgac ggtgatcagg tgcacgccct tgccggcgag cgcgttcaga tagaccggcg
      361 cggtggccac cagggtcttg ccctcgccgg tccgcatctc ggcgatgccg ccctgatgca
      421 acaccatgcc gcccaccagc tgcacgtcgt agtgccgctg gcccagcgtc cggcgtgcgg
      481 cctcgcgcac gaccgcgaag gcctcctcca gcaggtcgtc gagcgtctcg ccgttggcga
      541 accgcgcgcg gaactcttcc gtcttggccc tcagctgctc gttggagagc gcctggatct
      601 gcggctccat gtcgttgatc cggccgacac ggcccagcat ggtgcggacc ttgcggtcgt
      661 tggaggagcc gaagaaccgt tggaacatgg tcaagcggag aggtccggac tgatgggcgg
      721 cggcaggccg cacaggcccg aaaaaaccgc tcatttgaag gcgcgcgaga cttaagcagc
      781 gcccccttca gtgtcaacgc ggcccccgga cccattcccg ctcatcccgg cgttcgccgg
      841 gatgagcggt tatcgatcac agcacctcga acaatcctgc gccgcccatg ccggtgccga
      901 tgcacatcga gaccacgacg ttcttcacgc cgcggcgctt gccttcctgc aggatgtggc
      961 cggtaaggcg cgcgccggtc atgccatacg ggtggccgat ggcgatcgag ccgccgttga
     1021 cgttgtagat ctccggatcg acgcccaggg tgtcgcggca gtagaggcac tgcgaggcga
     1081 aagcctcgtt gagctcccag agctcgatgt catccacctt caggccgtgg cgctccagca
     1141 gcctgggcac cgcgaacacc gggccgatgc ccatctcgtc gggctcgcag ccggcgaccg
     1201 cccagccctt gaagacgccc atcggctcca ggccgcgccg ctcggcttcc ttcgcctcca
     1261 tgatcaccac ggcggccgcg ccgtcggaga gctgcgaggc gttgccggcg gtgatgaact
     1321 tgccctcgcc cttcaccggc tgcagcttgg ccaggccctc gagcgtcgtg tcggggcggt
     1381 tgcactcgtc gcggttcacc tcgtagtcga cgatcttctc ctcgcccgtc tccttgttga
     1441 cctgcttcat cttggtcttc atcgggacga tctcgtcgcg gaacttgccg gcctgctggg
     1501 cggcggccat ccggcgctgg ctctccagcg aatactcgtc ctggtactcg cggctgatct
     1561 tgtagcgcgc ggccaccacg tcggcggtgt cgatcatcgg catgtggaag gccgggtaca
     1621 tctgggtcag cgtcgggtcg ttgttctccg ccgagccgcg gccggggggc atgctgatgc
     1681 tctccacgcc gccggccacg accacctcgg cgccgtcgtt cttcacatag ttggccgccg
     1741 cggcgatggc gttgaggccc gacgagcaga agcggttgat ggtgacgccg gcggtggtca
     1801 ccggcagccc cgcaagcagc gccgcctggc gcgcggggtc gccgtttgga tgagccacgt
     1861 tgccaaggaa gacgtcctcg acggcctcct tctcgacgcc ggcgcggtcg accgcgtgct
     1921 tgatggcgtg ggcggccatg ctcatgaccg gggtcatgtt gaacccgccg cgggccgatt
     1981 tggccagtcc cgtccgggcg taagaaacaa tgacggcttc gcgcatttga aatgatcctc
     2041 cacaagaatt cttcggcggc cctgacgggg tcgatctgag cgctccgtct aacctttgca
     2101 tcgtgtggcg caaatggcta gggacggggc gcgcgccagc gcgatttcca taccgccggc
     2161 cgacgcgcgc aagacgccgc cgcatcagga caactcagac ggggggccgg tcatgagccg
     2221 caagccgagg accgccaagg cgctggaagt cgccgccaag ccggtggagg tcgtgggcca
     2281 ggcgctggag cgcgcgttcg atcctctgac cagcacgctc aagcgcgccg cgctcaagcg
     2341 cgccgacaag caggcccgcc agttcgccca ggctacgacg gcggcgccga cgaacaaggc
     2401 cgagcgcacc gatctcatgg tctcgccgct cgcggttccg tttcc
//
LOCUS       DASUBI010000149         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_23068, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000149 DASUBI010000000
VERSION     DASUBI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23068"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VFU72_02825"
     CDS             <1..1380
                     /locus_tag="VFU72_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5422450.1"
                     /translation="KWDVSSDGTQYTFHLRSGMKWSDGQPVKAHDFEYSWKRALTPSL
                     ASDYASALYPLKGALDFNTGKSTDPNSVQVSATDDLTLHATTDHAAPYFLHLCATWTY
                     LPVPKWAVNKSGDKWVEPGNIVTNGMFKLQDWKHDQEIVIVRNEDYWGQKPTLQQISF
                     PISADPFSTSVKDYEANAIDITDEIGPADITRVQNDPTFSKELHHYVWSGTAFVAFDC
                     GNTDSPVSKADFRQALYLATDQKSLCQSVLKGLYDPAPTVTPPDIVGYDPSAAPTGGV
                     AAAKQKLASAGYANGQGFPGVKLVWPADVRYDLLAQALQQMWKTNLGIDVTLQRMESK
                     EFNAAFNSWNKTHFEMYIDRWGSDYSDPYNWYPILWTSDQDFFRTHWKNAAFDQQVAA
                     ADLELDPDKRKAEYEKAETMLMQGMPVLPIYHLNVNAMVRPYVAGFKEPASAAQWRQA
                     YSGVKILEH"
     gene            1385..>2141
                     /locus_tag="VFU72_02830"
     CDS             1385..>2141
                     /locus_tag="VFU72_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422451.1"
                     /translation="MGAYIVRRVLLIIPTLLLVYTIAFFMVHATPGGPWDNAEKPLPP
                     AVIENIKVKYHLNDPIWKQYLEYLSGAVRGDLGPSYVNRSQDVADIMRRAFPVSLQLG
                     VTALVLGALIGIPLGTLAAVKQNTLTDYLAMFFAVVGVSTPNYVTASALVLILAVFLH
                     WLPPGGWHGVFSKTIIIPALALAFGPASALARYIRSSMLEVLRQDYMRTARAKGLSDR
                     VVIIGHGLRNALVPVITVSAFLVADVITGSFFVE"
BASE COUNT          399 a          721 c          646 g          375 t
ORIGIN      
        1 aagtgggacg tctcgagcga cgggacgcag tacaccttcc acctgcgctc gggcatgaag
       61 tggagcgatg gccagccggt gaaggcgcac gacttcgagt actcctggaa gcgcgccctg
      121 acgccgtcgc tcgcctccga ctacgcctcg gcgctctacc cgctcaaggg cgcgctggac
      181 ttcaacaccg gcaagagcac ggaccccaac agcgttcagg tgtcagcgac cgacgacctc
      241 accctgcatg cgacgaccga ccacgccgcc ccgtatttcc tccacctatg cgccacctgg
      301 acttacctgc ccgtgcccaa gtgggccgtc aacaagtcag gggacaaatg ggtcgagccc
      361 ggcaatatcg tcaccaatgg gatgttcaag ctgcaggact ggaagcacga ccaggagatc
      421 gtgatcgtac gcaacgagga ctactggggc cagaagccga cactgcagca gatcagcttt
      481 ccgatcagcg ccgatccgtt ctccaccagc gtcaaggatt acgaggccaa cgccatcgac
      541 atcaccgatg agatcggtcc ggcggacatc acgcgggtgc aaaacgaccc gaccttcagc
      601 aaggagctcc accactatgt gtggtcgggt accgccttcg tcgccttcga ctgcggcaac
      661 accgattcgc ccgtcagcaa ggcggacttc cggcaggcgc tctacctcgc gaccgaccag
      721 aagtcgctgt gtcagagcgt gctcaagggg ctctacgatc cggcgcctac ggtcacgccg
      781 cccgatatcg tcggctacga ccccagcgcc gcgccgactg gcggagtggc ggccgccaag
      841 caaaagctgg cgtcggcggg gtatgccaat gggcaaggct tccccggcgt gaagctcgtc
      901 tggccggcgg acgtgcgcta cgacctgctc gcgcaggcgc tgcagcagat gtggaagacc
      961 aacctgggca tcgacgtcac gctccagcgc atggaatcga aggagttcaa cgcggcgttc
     1021 aactcctgga acaagaccca tttcgagatg tacatcgacc gttggggctc cgactattcg
     1081 gatccgtaca actggtatcc gatcttgtgg acctccgacc aggacttctt ccgtacgcac
     1141 tggaagaatg ccgcgttcga ccagcaggtc gcggcggccg atcttgagct cgatccggac
     1201 aagcgtaagg cggagtacga gaaagcggag acgatgctca tgcagggcat gccggtcctg
     1261 cccatatatc acctgaacgt gaatgcgatg gtccgcccgt atgtcgcggg cttcaaagag
     1321 cccgcgtcgg ccgcgcaatg gcggcaagcc tactcgggag tgaagatcct ggagcattga
     1381 gcgcatggga gcctacatcg ttcgccgcgt gctcctgatc atcccgacgc tgctcctggt
     1441 ctacacgatc gcgttcttca tggtgcacgc cacgcccggc gggccgtggg ataacgccga
     1501 gaagccgctg ccgccagcgg tgatcgagaa catcaaggtc aaataccacc tgaacgaccc
     1561 gatctggaag cagtacctcg agtatctgtc tggcgcggtg cgcggcgatc ttgggccttc
     1621 ctacgtgaac cgctcacagg atgtggccga tatcatgcgc cgcgcctttc ccgtttcgct
     1681 ccagctcggc gtcaccgcgc tcgtgctcgg cgcgctgatc ggcattcccc tcggtaccct
     1741 ggccgccgtc aagcagaaca cgctgaccga ctacctggcg atgttcttcg ccgtcgtcgg
     1801 cgtctcgacg ccgaactacg tcacggcgtc ggccctggtg ctgatcctgg cggtctttct
     1861 ccactggctg ccgccgggcg gctggcacgg cgtcttcagc aagacgatca tcatccccgc
     1921 gctggcgctc gcctttggcc cggcgagcgc cctggcccgc tatatacgct cctcgatgct
     1981 cgaggtcctg cgccaggact acatgcggac cgcgcgtgcc aaggggctca gcgaccgagt
     2041 ggtgatcatc ggccacggcc tgcgcaacgc gctggtgccg gtcatcaccg tctccgcctt
     2101 cctggtggcc gacgtgatca ccggctcgtt cttcgtcgag a
//
LOCUS       DASUBI010000150         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_104956, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000150 DASUBI010000000
VERSION     DASUBI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104956"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..899
                     /locus_tag="VFU72_02835"
     CDS             285..899
                     /locus_tag="VFU72_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422452.1"
                     /translation="MTNAAQRALGDVFVVRTPEELKVVADPLRLRLLALLRQAPHTVK
                     ELAELLDLPPTRLYYHINQLEAPGLIRVVETRLVSGIVEKRYRASSARLSIDRALLGS
                     GVGGADPALEAMLAAILDGTREGILRSAQAGLIDPGVESPREGGLLLGRRWLRLTPEQ
                     AARLYDRLVALVQEFEAEAPAPDAPDHAYELLLGLYPTLEETNG"
     gene            892..2163
                     /locus_tag="VFU72_02840"
     CDS             892..2163
                     /locus_tag="VFU72_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422453.1"
                     /translation="MAEREAVRRDFWVFWAGQTISNLGNAITFLALPLLIFHLTGSAL
                     NLAIASATAFLPYPLFGLVIGAWVDRVDRRRLMLATDLARAAMLCSIPLLFELGDLVV
                     WWIYAVGFINSALGIAFNAAEFAAIPSLVDHSDLVSANGRIQASYSAATVAGPLVAGV
                     LFAVVAPATVLLLDAATFVISAVSLVLIHTKLNTAAPQEPTSLAAIRRDVVEGLRYVL
                     GHPVLRAISIMMALVNFVGASTGAQLVFFAKERFTASDGQVSLLYSAAGAGVVLFSLS
                     AGLLRRRWSFSRVALGALMLNGLLTLAFALLPGFWPALGVWALLSGAGVLFNINTGSL
                     RQTIVPNQLLGRVQSIAGVLAWSAIPLGSFLGGLAIEWSGSVALIYALIGVSVAAIAA
                     AFAFTALGHAEDYLPPEQAGSPAEPVEVGAR"
     gene            2618..>3303
                     /locus_tag="VFU72_02845"
     CDS             2618..>3303
                     /locus_tag="VFU72_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5422454.1"
                     /translation="MPLDDNLFPILPTSPISERLTSSTHIQHDLRVPLRDGVELSLDV
                     IRPDAPGAYPVVLLRTPYDKTQSRTPFLHELARRGYIVALNDLRGRFNSDGQFTPYIH
                     DTDDGYDVVEWLAAQAWCDGNVGMAGGSCVARTQWLAAAANPPHLRAIVPVVSPPDAY
                     LNEPITHGCFLLPVSEWMLWMGRRSWQLTDAEPIYTQLRPYFDTLPLADVARAAGIQS
                     HWWDEWMQHP"
BASE COUNT          462 a         1193 c         1104 g          544 t
ORIGIN      
        1 aggttagagt gagtcatcgc ggcccctcct tagttgatgt aatggtaaat ctgtgcggcg
       61 atactagcat ccgcggcggt gcctgtaaag tgtctctacc cacctgacgc agtcctatat
      121 cttgcgcggg cggcaggacc cccacctatc cccggcgccg ggtgtggcct ggaagctcca
      181 gtgagctgag cggcggcgcg gccccgggga tctccttgac gaatcgccaa gcaccggcta
      241 tcttcttact caagaagatt tgaacgctca cgtggaggtg aataatgacg aatgcggcac
      301 agcgggcgct cggcgatgtc ttcgtcgtgc gcacgccgga ggagctcaag gtcgtggccg
      361 acccgttgcg gctgcggctg ctggcgctct tgcgccaggc gccgcacacg gtcaaggagt
      421 tggccgagct gctcgatctc ccgccgaccc gcctctacta ccacatcaac cagcttgaag
      481 cgcccggcct tatccgcgtg gtcgagaccc gcctcgtctc cggcatcgtc gagaagcgct
      541 accgggccag ctcggcccgc ctctcgatcg accgcgcgct gctggggtcc ggcgtcggag
      601 gggccgaccc cgcgctggag gccatgctgg cggccatcct cgatggcacg cgcgagggca
      661 tcttgcgcag cgcccaggcc gggctgatcg accccggcgt cgagtcgccg cgcgaggggg
      721 gtctgctgct cggccgccgc tggctgcggc tgacgccgga gcaggccgcg cggctctatg
      781 accgcctggt ggcgctggtg caagagttcg aggccgaggc gccggcgcct gatgctccgg
      841 accacgccta cgagctgctg ctggggctct accccacgct ggaggagacc aatggctgag
      901 cgcgaggcag tgcggcgcga cttctgggtc ttctgggccg gacagaccat ctccaacctg
      961 ggcaacgcca tcaccttcct ggccctgccg ctcttgatct tccacctgac cggctcggcg
     1021 ctgaatctgg ccatcgccag cgccaccgcc ttcctcccct atcccctctt cgggctggtc
     1081 atcggcgcct gggtcgaccg ggtcgaccgc cggcgcctga tgctcgccac cgacctggcc
     1141 cgcgcggcca tgctctgctc gatcccgctg ctcttcgagc tgggcgacct ggtagtctgg
     1201 tggatctacg cggtgggctt catcaacagc gccctcggca tcgccttcaa tgccgccgag
     1261 ttcgccgcca ttcccagcct ggtcgaccac agcgacctgg tcagcgccaa cggccgcatc
     1321 caggccagct actcggccgc caccgtcgcc gggccgctgg tggccggcgt cctcttcgcc
     1381 gtggtggcgc cggcgaccgt gctgctgctc gacgccgcca ccttcgtaat ctccgccgtc
     1441 tcgctcgtgc tgatccacac caaactcaac accgccgcgc cccaggagcc gaccagcctc
     1501 gcggcgatac gccgcgacgt ggtcgagggg ctgcgctacg tgctggggca tcccgtgctg
     1561 cgcgccatct cgatcatgat ggcgctggtc aacttcgtcg gcgcatccac cggcgcgcag
     1621 ctcgtcttct tcgccaagga gcgcttcacc gccagcgacg gccaggtcag cctcctctac
     1681 tcggccgccg gcgccggcgt ggtgctcttc tcgctctcgg cggggctgct gcggcgccgc
     1741 tggtcgttca gccgggtggc gctgggcgcg ctgatgctca acggcctgct gacgctggcc
     1801 ttcgcgctgc tgcccggctt ctggccggcg ctcggggtct gggcgctgct cagcggcgcg
     1861 ggggtgctct tcaacatcaa caccggcagc ctgcggcaga cgatcgtgcc caatcagttg
     1921 ctcgggcgcg tgcagagcat cgccggggtg ctggcctggt cggccatccc gctcggcagc
     1981 ttcctgggcg ggttggcgat cgagtggagc ggcagcgtgg cgctgatcta cgcgctgatc
     2041 ggcgtgagcg tggccgccat cgccgccgcc ttcgccttca ccgccctcgg ccacgccgag
     2101 gactacctgc cgccggagca agccggcagc ccagcggagc cggtcgaggt gggcgcgagg
     2161 tgagcctcgc tgacccccgc gttgcccgtg tgccggctgc aaacgcagcc tgcgccaagc
     2221 tccggctgac ccagcggtga ggatatcgac gcacaggccg cgcccaccgt cgtcgttcgc
     2281 accaatctgg ctcctgctgt tcagagaagc accgcaaggg gagtcaggaa cggcgcgagg
     2341 cgactcgtgc cccgagtctg gagaacggcg acgtgccgct gcctggtcat tcaatcgccc
     2401 cgccggcggc aagcgcaccg agccatcagg cggcccatca cgccgccggc ttgcggcgcg
     2461 gagcggacgc gcgatactgc ggtcgggatc gagacgctgg ccgggccgct caacgcgggg
     2521 gcgagatcgg accgagcggc ggtccgaaga cgattgccgg cagggggcct ggctccgctt
     2581 cgaaatgaga gcaggattcc tgttcacgga ggtgacgatg cccctcgacg acaacctgtt
     2641 cccgatcctg ccgaccagcc cgatctccga acggctgacc agcagcacgc acatccagca
     2701 cgatctgcgg gtgccgctcc gcgacggcgt cgaactctcc ctggacgtga tccggcccga
     2761 tgcgccgggg gcgtatccgg tcgtgctcct gcgcacgccg tacgacaaga cgcagagccg
     2821 caccccgttc ctgcacgagc tggcgcgccg gggctacatc gtcgccctga acgatctgcg
     2881 cgggcggttc aattcggacg gccagttcac gccctacatc cacgacacgg acgacggcta
     2941 cgatgtcgtc gagtggctcg cggctcaggc gtggtgtgac ggcaacgtcg gtatggccgg
     3001 cgggtcctgc gtggcgcgca cccagtggct ggccgcggcg gcgaacccgc cgcatctgcg
     3061 cgcgatcgtc ccggtcgtgt cgccgccgga cgcctatctg aacgagccga tcacccacgg
     3121 ctgcttcctc ttgccggtca gcgagtggat gctctggatg ggccggcgct cctggcagct
     3181 gaccgacgcc gagccgatct acacccagct gcggccgtac ttcgacaccc tgccgctggc
     3241 cgacgtcgca cgggcggccg gcatccagtc gcactggtgg gacgaatgga tgcagcaccc
     3301 caa
//
LOCUS       DASUBI010000151         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_732095, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000151 DASUBI010000000
VERSION     DASUBI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732095"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VFU72_02850"
     CDS             <1..1552
                     /locus_tag="VFU72_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5422455.1"
                     /translation="TISPGAIVDLARGTRFGWLWLARIALLLGAAALLARPAAWASTR
                     RRWRWPAIVLAGALFLPYALVSHAAAQTAGSPAAVSADWLHLLGTAIWIGGLLALLAG
                     LVYNPALPAESRRSVYASAIQHFSTVAIAAVVVLVLTGLYAAWLEVGSLEALRATRYG
                     HALDIKLLLLVPLLGLASYNLMVLGPAMLRVPSAAVGFARTVLGEATFGVALLLVVGV
                     LMGLPTGRQVTSQPSDHPLLRFEHDGIAAVVQLTPGTVGLNRYTVDVTRNGRPLPENY
                     KVLLDVSSSQLNGLREVELTRSAGELERWEAQGSELSVAGEWQVRLIVRPPDVDDWGA
                     IRTLEIGRSYTPAPPQPVSRFLGYLPALLVLLIAGGVALLVVGLRFHGSRETRRLVLE
                     LGAGLLIIGAVTLALTLGTSENAAAATLNRMPPTSVSISRGQHVYRQECAGCRGVDLR
                     GDGPGAAGLNPPPAGLTRHAGAHPDVQLFTWISDGMPNSAMPAFADRLTSPQRWDLVN
                     SLRSATGS"
     gene            1920..2180
                     /locus_tag="VFU72_02855"
     CDS             1920..2180
                     /locus_tag="VFU72_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422456.1"
                     /translation="MADGPSWAALTLVDWIGLIVVLLFWVLVPIAIYLLVSRGPSPRA
                     ADDAETWDWISHARLFPPEDAEQSASESAGAGLGEAAPAHGT"
BASE COUNT          298 a          814 c          819 g          401 t
ORIGIN      
        1 cacgatctct ccgggcgcta tcgtcgacct ggcgcgcggc acgcgcttcg gctggctgtg
       61 gctggcgcgg atcgcgctgc tgcttggcgc ggcggcgctg ctcgcgcgcc ccgctgcctg
      121 ggccagcacg cgacgccgct ggcgctggcc ggcgatcgtg ctggccggcg cgctctttct
      181 cccctatgcg ctggtcagcc acgcggcggc gcagaccgcc ggcagcccgg ccgcggtgag
      241 cgccgattgg ctgcacctgc tgggcaccgc gatctggatc ggcgggctgc tggcgctgct
      301 ggccgggctg gtctacaacc cggcgctgcc cgccgagtcc cgccgatcgg tgtacgcgag
      361 cgccattcag cacttctcga ccgtggccat cgcggccgtt gtcgtgctgg tgctgaccgg
      421 actctatgcc gcctggctgg aggtcggctc gctggaggcg ctacgcgcca cccgctatgg
      481 gcacgcgctc gacatcaagc tgctcctctt ggtcccactg ctggggttgg cttcctacaa
      541 tctcatggtg ctcggcccgg cgatgctgcg ggtaccgtcg gccgccgtcg gcttcgcccg
      601 taccgtcctg ggtgaggcga ccttcggcgt ggcgcttctg ctggtggtcg gcgtgctcat
      661 gggtctgccg accgggcggc aggtcaccag ccagccgagc gatcacccgc tgctgcgctt
      721 cgagcacgat gggatcgcgg ctgtggttca actcaccccc ggcacggtcg ggctgaaccg
      781 ttacacggtc gacgtgaccc gcaacggccg cccgctgccc gagaactaca aggtcctgct
      841 cgatgtctcc agcagtcagc tcaacgggct gcgggaggtc gagctcaccc gctcggccgg
      901 cgagcttgag cgctgggaag cgcagggaag cgagctgagc gtggccggcg agtggcaggt
      961 gcgcctgatc gtgcgcccgc cggacgtcga cgactggggc gcgattcgca cgctggagat
     1021 cgggcgcagc tacaccccgg cgccgcccca gccggtgagc cgcttcctgg gctacctgcc
     1081 ggcgctgttg gtgctgctga tcgccggagg cgtggcgctg ctggtggtgg ggctgcgctt
     1141 ccacggttcg cgggagacgc ggcggctggt gctggagctg ggagccgggc tgctgatcat
     1201 cggcgcggtg acgctggcgc tgacgctggg gacaagcgag aacgcggcgg cggcgacgct
     1261 caaccggatg ccgccgacgt ccgtctcgat ctcgcgcggc cagcacgtgt accggcagga
     1321 gtgcgccggc tgccgcggcg tggacctgcg cggcgacggt cccggcgctg ccgggctcaa
     1381 tccgccaccg gccggtttga cccggcacgc gggcgctcac cccgacgttc agctcttcac
     1441 ctggatcagc gacggcatgc ccaacagcgc catgccggcc ttcgcggatc gcctcacctc
     1501 accgcagcgc tgggatctgg tcaactccct ccgctcggca acggggagct gagcaggcgc
     1561 ggcggctcgg cgaacacgtt ggctgccatg tgcggcacgg atccctccac tgcggtcggg
     1621 atgacagggt taattggggg cggacgacaa cgactcccgt cgtcgtccgc ctcgtccttt
     1681 ttccctgtcc tcctgagcgc agtcgaagga tccgtgccgc acatggcacg aaaccttctc
     1741 gatgggcagc ggctcaccga agctgcgcag ccacaccgtt caattcccgc gagcgatctt
     1801 gacatccggg cgcacgggtg taatctcgtg gagcagagta tgcgaatcga cgcgatgggg
     1861 gttggagcag tgtagcgagc tgagggctcc gtcgtagcca cggatcggag gtacttccca
     1921 tggctgatgg accgagttgg gccgctttga cacttgtcga ctggatcggc ctgatcgtcg
     1981 ttctcctctt ctgggtattg gtcccgatcg ccatctacct gctggtctcg cgtgggccga
     2041 gcccgcgcgc cgccgacgat gcggagacct gggactggat cagccacgcc cggctcttcc
     2101 cgcccgagga cgccgagcag agcgcgtcgg agagcgccgg agccggtctt ggagaggcgg
     2161 cgcccgccca cggcacctga gtcgtccggt gcaatcaccg gatcgtaagc ctccccgtcg
     2221 cgtaccgatt gcaggccagg atcgcgtacc cgttccgcca ctcgcctcgt gaccgtccgg
     2281 atgccgggcc gggagcgcgc cagcgctccc gatcccctct gcttcttctt ca
//
LOCUS       DASUBI010000152         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_404791, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000152 DASUBI010000000
VERSION     DASUBI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404791"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="thyX"
                     /locus_tag="VFU72_02860"
     CDS             complement(<1..738)
                     /gene="thyX"
                     /locus_tag="VFU72_02860"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5422457.1"
                     /translation="MRVPVRVAIDHAAGRGRPKGSLAMDHVHVLDHGYVRLVDVLGDD
                     LLVVNAARVSFDKESSWQEAGGGDAGAERSLRARDQRLLDFLVREGHTSPFRHPTLSF
                     EVYAPLMVMRQWGKYRIGSVWAFQDSDDPRETWNESSRRYVTEEPVCYCPEEWREAPA
                     NKKQGSGAVLPASPVLHDLLRRTIEEGQARYAEAMAAGVCAEQARLFLPAYALYLRAR
                     WTVSLQGVIHFLRERQEDGAQYEIRAYA"
     gene            complement(935..1126)
                     /gene="rpmB"
                     /locus_tag="VFU72_02865"
     CDS             complement(935..1126)
                     /gene="rpmB"
                     /locus_tag="VFU72_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5422458.1"
                     /translation="MAKCEICGKGPSFGHNVSHSHHKTNRMFRPNIQKTTIYKDGQAR
                     KLKVCTRCLRTLYKQARTA"
     gene            1374..>2131
                     /locus_tag="VFU72_02870"
     CDS             1374..>2131
                     /locus_tag="VFU72_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU5422459.1"
                     /translation="MSRTETTERADRSARWSGADLLAALDFAVAYFEPFVPELNALNV
                     FPVPDGDTGTNMFLTLQAAGREADRLGPGGSAGEVLAAAAHGALMGARGNSGVILYQS
                     LTGLAEALQGEPVVTGAGLAAGLTRAGELVYQAVLKPVEGTMLTVMREAGAAARRAAE
                     AEHSLGPVLDAALAGAETALARTPEQLPILRQAGVVDAGGRGVVVFLAGLRAFVNGES
                     LDHGVAQPAKPLGQAMSFLDIPEIVHDAEEFGYCP"
BASE COUNT          321 a          736 c          742 g          332 t
ORIGIN      
        1 ggcgtaggcg cggatctcgt actgcgctcc atcttcctga cgctcgcgca ggaagtggat
       61 caccccctgc aagctgaccg tccagcgtgc gcgcaggtag agcgcgtagg ccgggaggaa
      121 gaggcgggcc tgctcggcgc agacgcccgc ggccatcgcc tcggcgtagc gcgcctgccc
      181 ctcttcgatg gtgcgccgca gcagatcgtg gagcacgggg ctagccggca gcaccgcgcc
      241 gctgccctgc ttcttgttgg ccggcgcctc acgccactcc tccgggcagt agcagaccgg
      301 ctcttcggtg acgtagcggc gcgacgactc attccaggtc tcgcgcgggt cgtcgctatc
      361 ctgaaaagcc cagacgctgc cgatccggta cttgccccac tggcgcatca ccatcagcgg
      421 cgcgtagacc tcgaacgaca gcgtcgggtg gcgaaacggg ctggtatgcc cctcgcgtac
      481 caggaagtcg agcagccgct ggtcgcgggc tcggagcgag cgctcggcgc cggcatcccc
      541 gccaccggcc tcctgccagc tggactcttt gtcgaagctg acgcgggcgg cgttgacgac
      601 caggaggtcg tcgcccagga catcaacgag ccgcacatac ccgtgatcga gtacgtggac
      661 gtgatccatc gccaggctcc ccttcgggcg gccgcggccg gcggcgtggt cgatcgcgac
      721 ccgaaccggc acacgcacct gccgcgtccc ccgcgtgtca tgcgcgcctc agcgtatctt
      781 ctcaatcgag tgtagcacgc cgttcaacgg cgacaggcgg cggtgaagcc agcgagaagc
      841 cacgcctgcc gcgcgtacca gccgcgacac tgcgcgagca gacgacaacg ccctcccgta
      901 tgggagggcg ttcagcgtcg gagagaagac ggcgctacgc ggtgcgggcc tgcttgtaga
      961 gcgtgcgcag gcagcgggtg caaaccttga gcttcctggc ctgcccgtcc ttgtagatcg
     1021 tcgtcttttg aatgttcggc cggaacatgc ggttcgtctt gtgatgcgag tggctgacgt
     1081 tgtggccgaa cgacggcccc ttgccgcaaa tctcacactt ggccatctcg gtcccccttt
     1141 accagtcttg ccggaggaaa taccatgttc atcgcgttgc cggggcgacg agattgaccg
     1201 cgaacgccca cgaactcgaa ggataccacc ggccggtggc cggcacaata cggcctgcgg
     1261 ctccgcgatt catggcgcgt gctgccagcg gtggcgttca accgcgccag atggtatgac
     1321 ccttgcgcgg gggtagggcc agccggggtg ttggggtgag gatgactatg gagttgagca
     1381 ggacggagac gacggagcgt gcggaccggt ccgcacgctg gagcggcgcc gaccttctgg
     1441 cggcactcga cttcgccgtc gcctatttcg agccgttcgt acccgagctc aacgccctca
     1501 acgtcttccc ggtccccgac ggcgacaccg gcaccaacat gttcctcacc ctgcaggcgg
     1561 ccgggcgtga ggccgaccgg ctcgggccgg gaggctcggc gggagaagtc ctggccgcgg
     1621 cggcgcacgg cgcgctgatg ggcgcgcgcg gcaactccgg cgtgatcctc tatcagtcgc
     1681 tgaccggcct ggccgaggcg ctgcagggcg agccggtcgt gaccggcgca ggcctggcgg
     1741 ccggtctgac ccgcgccggc gagctggtct accaggccgt gctcaagccg gtcgagggca
     1801 ccatgctgac cgtcatgcgc gaggcgggcg cggccgcgcg gcgcgcggcg gaggcagagc
     1861 actccctcgg ccccgtcctc gacgccgcac tggcgggcgc tgaaaccgcg ctggcgcgca
     1921 cgcctgagca gttgccgatc ctgcgccagg cgggcgtggt cgacgccggg gggcgcggcg
     1981 tggtcgtgtt tctggccggg ctgcgcgcct tcgtgaacgg cgaatcgctc gatcacggag
     2041 tagcccaacc cgccaagccg ctcgggcagg ccatgagctt cctcgacatc cccgagatcg
     2101 tgcacgacgc cgaggagttc ggctactgcc c
//
LOCUS       DASUBI010000153         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_296823, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000153 DASUBI010000000
VERSION     DASUBI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296823"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFU72_02875"
     CDS             complement(<1..931)
                     /locus_tag="VFU72_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5422460.1"
                     /translation="MLGTLQPGTVQRHEVTLPGAALAGERWPVISVSGVVPGPALFVN
                     AGVHGGEYPAVEAVIRLGQLLNPQELAGTVVLLPVLNLPAFRQRSMYVCPVDNLNPNR
                     QFPGSPGGSYSQQLVHALTEEFIARADYYIDLHGGDIVEDLAPFAICRQGSDPVDQRA
                     LELARLFGLPYVLAVDSSFQAGSGTLSYNAGAARGVPSLIAEAGGLGLLQPEAVELLV
                     NGVLRVLAHLGMRAATVSAAPAPTVLSEFRWIYSQHEGLFYPTVTINDQVRQGQVVGR
                     IGSLFGDTLEEVTAPVSGPVLFVMVNPSVPANGL"
     gene            complement(1125..2168)
                     /locus_tag="VFU72_02880"
     CDS             complement(1125..2168)
                     /locus_tag="VFU72_02880"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5422461.1"
                     /translation="MRVTVVGAGAIGGTAGALLHQAGHQVTLVDRDEAHVAAINRDGF
                     RLSGAVELVEPVPAITAERLPELVQHEGPLGMVLLAVKALDTEAAVEEIRPHLAPEGF
                     IVSYQNGLNEDLIACMVGRERTIGAFIHYGGDLVEPGHVVLANRVPSYLSELDGQETP
                     RIREVANILSAVTPAIITDNLEGYLWGKLCYASLAFAVSIVDAPTDEVVSVPQARRVL
                     RQLVAEVVDVAHAQDIRLENIHGFEPAIFDRVNPDRIAESDALFDRWAARGKTAIKRH
                     MGIHRDIKVRRRRTEVDFQVGPVVERGAAVGVATPLNALLQHLVHQVESGERPQDWSN
                     IEALDEAAATLGA"
     gene            complement(2255..>2695)
                     /locus_tag="VFU72_02885"
     CDS             complement(2255..>2695)
                     /locus_tag="VFU72_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5422462.1"
                     /translation="EQGARALPPGAPHLEAWRQKAIANAWKTAGRAKELGVPIICGTD
                     AAMPYVTHGGNAYELEMLVEAGLTPGEALAAATSGAADAIAFPEVGRIAAGNYADLVL
                     VDGDPLADIRVLQDRRRISLVLKSGAIVADRRAQVAAPVPAPAD"
BASE COUNT          401 a          911 c          976 g          407 t
ORIGIN      
        1 agaggccatt ggccgggacc gaggggttga ccatgacgaa gaggaccggg ccgctgaccg
       61 gcgcggtgac ctcttccagg gtgtcgccga agagcgaacc gatgcgaccg acgacctgtc
      121 cctggcggac ctggtcgttg atcgtcaccg tggggtagaa gagcccctcg tgctgggagt
      181 aaatccagcg gaactcgctc agcacggtgg gcgcgggcgc ggcggacacc gtggcggcgc
      241 gcatgccgag gtgggccagc acccgcagca cgccgttgac caggagctcg accgcctccg
      301 gctgcagcag gccgagcccg cccgcctcgg cgatgaggct gggtacgccg cgcgccgccc
      361 cagcgttgta gctcagcgtg ccgctgccgg cctggaacga gctgtcgacc gccagcacgt
      421 agggcaggcc aaagagccgg gccagctcca gcgcgcgctg atcgaccggg tcgctcccct
      481 ggcggcagat cgcgaagggg gccagatcct cgacgatgtc gccgccgtgc aggtcgatgt
      541 agtaatccgc ccgggcgatg aactcttcgg tcagggcgtg gaccaactgc tggctgtagc
      601 tgccgcccgg cgagccggga aactggcggt tggggttgag gttgtcgacc gggcagacgt
      661 acatgctgcg ctggcgaaag gccggcaggt tgagcaccgg cagcagcacg accgtgccgg
      721 ccagctcttg ggggttgagc agttggccca gccggatgac cgcctcgacc gccgggtact
      781 cgccgccgtg cacccccgcg ttgacgaaga gcgccggccc gggcaccacg ccgctgacgc
      841 tgatgaccgg ccagcgctcg cccgccagcg ccgcccccgg tagcgtcacc tcgtgccgct
      901 ggaccgtccc cggctggagc gtgccgagca cagggaaacg cgcggcggat gcacctgaag
      961 cagaactcac ggggcggccc tttcttcaca tctgaactgg gaatagcgat gagatccggg
     1021 gtcacgcggt cgacggtgta cgcattaaac tcggtgcgcg tttcgctttg cccaactgcc
     1081 acgcttccgc atagtaggaa gaaggcggtc gtctgtcaag atggttacgc gccgagcgtg
     1141 gccgcggcct cgtctaacgc ctcgatattc gaccagtcct ggggccgctc gccgctctcg
     1201 acctgatgca cgaggtgctg caggagggcg ttgagcggcg tggcgacacc cacggccgcg
     1261 ccgcgctcga ctaccgggcc gacctgaaaa tcgacctcgg tgcggcgccg gcgcaccttg
     1321 atgtcgcggt ggatgcccat gtggcgcttg atggccgtct tgccgcgcgc ggcccagcgg
     1381 tcgaagagcg cgtcgctctc ggcgatgcgg tcggggttga cgcggtcgaa gatggccggc
     1441 tcgaaaccgt ggatattctc cagccggatg tcctgggcgt gtgccacgtc caccacctcg
     1501 gccaccagct ggcggagcac gcgccgcgcc tgcggtacgc tgacgacctc gtcggtcggg
     1561 gcgtccacga tcgagacggc gaaggcgagc gaggcatagc agagcttgcc ccagaggtag
     1621 ccttcgagat tatccgtgat gatcgccggc gtgacggccg agaggatgtt cgcgacctcg
     1681 cggatgcgcg gcgtctcctg gccatccagt tcgctgaggt agctgggcac ccggttggcg
     1741 agcaccacgt ggcccggctc gaccaggtcg ccgccgtagt ggataaaggc gccgatagtg
     1801 cgctcgcggc cgaccatgca ggcgatcagg tcctcgttga gcccgttctg ataggagacg
     1861 atgaagccct ctggcgccag gtggggccgg atctcctcga cggcggcctc ggtgtcgagc
     1921 gccttgaccg ccagcagcac catgccgagc ggcccctcgt gctggacgag ctcggggagc
     1981 cgctcagcgg tgatggccgg caccggttcg accagctcga ccgctccact gagacgaaat
     2041 ccgtcgcgat tgattgcggc gacatgggcc tcgtcacggt cgaccagcgt gacctgatgc
     2101 ccggcctggt gcagcagcgc tcccgccgtg ccgccgatgg ccccggcccc gacaactgtg
     2161 acacgcatgg tgacctcctt tcggtcgctt gacttcagcc gggatgccct cacccccagc
     2221 ccctttctcg atgcgcggga gaggggagcg gcggctaatc cgccggcgct gggacgggcg
     2281 cggcgacctg ggcgcggcgg tcggcgacga tggcgccgct cttgagcacc agcgagatgc
     2341 gccggcggtc ctgcaggacg cggatatcgg cgagcggatc gccgtcgacc aggacgaggt
     2401 cggcgtagtt gccggccgcg atgcggccga cctcggggaa ggcgatggcg tccgccgcgc
     2461 cgctggtggc ggcggccagg gcctcgcccg gcgtcagccc ggcctcgacc agcatctcca
     2521 gctcgtaggc attgccgccg tgggtgacgt agggcatggc cgcgtcggtg ccgcagatga
     2581 tgggcacgcc cagctctttg gcccgcccgg cggtcttcca ggcgttggcg atcgccttct
     2641 ggcgccaggc ctccaggtga ggtgcgccgg gcggcagggc gcgcgcgccc tgctc
//
LOCUS       DASUBI010000154         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_241123, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000154 DASUBI010000000
VERSION     DASUBI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241123"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1110)
                     /locus_tag="VFU72_02890"
     CDS             complement(250..1110)
                     /locus_tag="VFU72_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422463.1"
                     /translation="MGETIRLPDGRALGFDEFGAHGGAPVIYLPGYGSSRLAAYPDDA
                     LTASTGARLIAVDRPGIGLSSPSDGYRPLDVAADVAALADALGLLRFALIGWSWGGAY
                     ALACGRAFPERVTRLGLASPMGGWLTGPGASTAVSPSFRQLALLVRLAPRALRLFLRR
                     QRRALLRDPEGAVTRAVHKLPLADRAVVGRPGMRTMLTANLLAAWRQGIDGVYQDTLA
                     VARPWGFAPQEVRLPVLLWQGEDDAEILPAMAHELVAALPDCRASFFMGEGHLVIYIH
                     WAEILSALVS"
     gene            1234..>2175
                     /locus_tag="VFU72_02895"
     CDS             1234..>2175
                     /locus_tag="VFU72_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5422464.1"
                     /translation="MSESEPRFDVSLARSIMVPMRDGVRLATDIYRPAEGVEPLPGSF
                     PTLLIRTPYDKGAADTIRRKGAYFASRGYAVVIQDVRGRYESEGEFYFLAQEAEDGYD
                     TIVWVGRQPWCDGKVGTLGTSYLAWTQSALAALNPPHLAAMYVNEGGANAYTSTVRQN
                     GAFELRFLAWAFMGAATSPGVPQAIRDALVASTPFSRWVRELPLKPGVSPLRLVPEHE
                     RWVLDIMYRGDYDEFWKHPGFNFEEHAEAHADVPILFSGAWYDSYTRATTENFVRFGE
                     IKRGPVRLLMGPWTHGGYPPDLTWSGDVEFGPTASIGG"
BASE COUNT          333 a          792 c          769 g          281 t
ORIGIN      
        1 gcgcggcgat atggctgtag ggcaccgcga tcgtgggcag gtcgggccgc tcgacgatga
       61 agttggactg ctccagcgcg accgcacggc cgaccggcag cccatcctcg gccacgaccg
      121 gcatccccag ctgtagtcgc gcgcgatctc gttcgataga catgtcttgc tccctccagc
      181 ggatctcctg cgcgggcgct cggcacccat cggctcacga tgcaactggc gtgccgggcc
      241 tattcgagct caactgacca gcgcgctcag gatctcggcc cagtggatgt agatcaccag
      301 gtgcccctcg cccatgaaga agctggctcg gcagtctggc agcgccgcga ccagttcatg
      361 ggccatggcc ggcaggatct cggcgtcgtc ctccccctgc cagaggagca ccggcagccg
      421 cacctcctgc ggcgcgaagc cccaggggcg ggcgaccgcc agcgtatcct ggtagacgcc
      481 gtcgatcccc tggcgccagg cggccagcag gttggcggtc agcatggtgc gcatgccggg
      541 gcggccgacg accgcgcggt cggccagcgg cagcttgtgg acggcgcggg tcacggcgcc
      601 ttcggggtcg cgcagcaggg cccgccgctg ccggcgcagg aagagtctca aggcgcgcgg
      661 cgccagccgg accagcaggg cgagttggcg aaagctcggg gagacggccg tgctcgcgcc
      721 ggggccggtc agccagccgc ccatcgggct ggccaggccg agccgcgtca cccgctcggg
      781 gaaggcgcgg ccgcaggcca gcgcgtaggc gccgccccac gaccagccga tcagcgcgaa
      841 gcgcaagaga ccgagcgcgt cggccagcgc cgccacgtcg gcggcgacgt cgagcggccg
      901 gtagccgtca cttgggctgg agagcccgat acccgggcga tcgaccgcga tcaggcgcgc
      961 gccggtcgaa gccgtcagcg cgtcgtccgg gtaggccgcc agccgcgagc tgccgtagcc
     1021 aggcaggtag atgaccggcg cgccgccgtg cgcgccgaat tcgtcgaagc cgagcgcccg
     1081 gccgtcgggc aggcggatcg tctctcccat aaccatcctt gaaggcgccg taccgcatcg
     1141 agcgcggctc gtcgcggcac cgcaaagggc gaaccagcag cctgggtctg ctagactgcg
     1201 cctgaccgag aagcgagcgg aaggagtgcg acaatgagcg agagcgagcc acggttcgac
     1261 gtcagcctgg cccgcagcat catggtgccg atgcgcgacg gcgtgcgcct ggcgaccgac
     1321 atctaccgcc cggccgaggg ggtcgagccg ctgcccggct cattcccgac cctgctgatc
     1381 cgcacgccct acgacaaggg cgcagccgac acgattcgcc gcaagggggc ctacttcgcc
     1441 agccggggct acgcggtcgt catccaggac gtgcgcgggc gctacgagtc ggagggcgag
     1501 ttctacttcc tggcgcagga ggccgaggac ggctacgaca ccatcgtctg ggtcggccgg
     1561 cagccctggt gcgacggcaa ggtgggcacg ctgggcacct cgtacctggc ctggacgcag
     1621 agcgcgctgg ccgcgctcaa cccgccgcac ctggctgcga tgtacgtcaa cgagggcggc
     1681 gccaacgcct acaccagcac ggtgcggcag aacggcgcct tcgagctgcg cttcctggcc
     1741 tgggccttca tgggcgcggc caccagcccc ggcgtgccgc aggcgatccg cgacgcgctg
     1801 gtcgcgagca cacccttcag ccgctgggtg cgcgagctgc cgctcaagcc cggcgtctcg
     1861 ccgctgcggc tggtccccga acacgagcgc tgggtgctcg acatcatgta ccgcggcgac
     1921 tacgacgagt tctggaagca ccccggcttc aacttcgagg agcacgccga ggcgcacgcc
     1981 gacgtgccga tcctcttctc cggtgcctgg tacgacagct acacccgcgc caccaccgag
     2041 aacttcgtgc gcttcggcga gatcaagcgc ggcccggtgc gcctgctgat gggcccctgg
     2101 acccacggcg gctacccgcc cgacctcacc tggtccggcg acgtcgagtt cggcccgacc
     2161 gcctcgatcg gcggc
//
LOCUS       DASUBI010000155         5205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1238446, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000155 DASUBI010000000
VERSION     DASUBI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238446"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1857
                     /locus_tag="VFU72_02900"
     CDS             163..1857
                     /locus_tag="VFU72_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU5422465.1"
                     /translation="MSTPATESTILPGSAPLVGQEITDNLEDLLAVLPPHITKGIRAL
                     HTAETRSDLIEIVLDLGRRPEARFTDRELYLSDDEVTREDIAFVAERIGRFGDDNRAG
                     IERTLHRISAIRSRSGEIVGLTCRVGRAVYGTIGIIRDLVDSGASILLLGRPGVGKTT
                     MLRETARVLADDLRKRVVVVDTSNEIAGDGDIPHPSIGRARRMQVPTPALQHAVMIEA
                     VENHMPEVIVIDEIGTELEAAAARTIAERGVQLIGTAHGNTLENLMMNPTLSDLIGGI
                     QSVTLSDEEARRRGTQKSILERKAPPTFAVMVEIQTRDRVAVHRDVAQSVDAILRGQT
                     LRVEVRERQPNGEVRVWQEQPYEAEHPTLVRQNSRNRQPAGSSNEIAPAEARPLAPRR
                     PPKSIRIFPFGVSRNRLEESISQLAVPATVVRELREADAVVTLKNYYRRQPHPLRDAE
                     ARGLPVYVLRNNTVAQIGALLRSLFQESAQGEPRAPQGPYSDVDPNRSPEDQVTAAMF
                     EAEDAIHHVMDGATSVELRPQAAWVRRLQHELAERYNVPSRSRGREPQRYVEFFRR"
     gene            1866..2492
                     /gene="tmk"
                     /locus_tag="VFU72_02905"
     CDS             1866..2492
                     /gene="tmk"
                     /locus_tag="VFU72_02905"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5422466.1"
                     /translation="MTLEGPEGGGKSTVGGRLREALEARGKSVVLTREPGGTPLGEAV
                     RDLLLGARHSTMQPEVEALLNSAARAQHVAEVIAPALDEGKIVISDRFADSTLAYQGA
                     GRGLDLNALAGVQRFATRGLTPELTLLFDVPYEVGQARRRESGELNRFDTDSRAFHER
                     VRAYFLWAARREPERWRVIDAAAPLDEVAEQALRLVLDHLDGQSMRVG"
     gene            2500..2832
                     /locus_tag="VFU72_02910"
     CDS             2500..2832
                     /locus_tag="VFU72_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU5422467.1"
                     /translation="MKLVFAVIQGRDADNLLAALRDAGYRSTLVSSAGGFLREQNVTV
                     LIGVEDQYVPDVMRLIKQNCYTRTRYVNPLLPIMEPGEFYVPDPVEVQVGGATVFVLN
                     VRYVERVI"
     gene            2875..3657
                     /locus_tag="VFU72_02915"
     CDS             2875..3657
                     /locus_tag="VFU72_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5422468.1"
                     /translation="MAALIARGVQLLIALGVAYFLALWFALIVWTFQDIQSRSRSVIA
                     QIFSTLVVVLFSFPGVILYLILRPRDTLDDAFQRSLEEEYLLQDLEELPLCPTCQHYV
                     EQDYVFCPHCRTELKEPCAGCGRPIDIRWEICPFCGEEQFEEELPAARLAEEWEPAEV
                     DPVTRLLGQARERLSGRRTRELGEGVGADERGMAELQQGGQSYLPWESEAPTVDTWAR
                     RPEPNALRGEREPRWGDDGAGPGEGDTYDPTEEIPIAPPPRP"
     gene            complement(3854..4702)
                     /locus_tag="VFU72_02920"
     CDS             complement(3854..4702)
                     /locus_tag="VFU72_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU5422469.1"
                     /translation="MGLRIGVIPARSKPEAAELAEELMPWLQAAGCAVVSEDALLRGD
                     SADLLLTIGGDGLIMRLAHNVPDVPILGINLGKVGFLARIERSAWRPALSEVVEGRYR
                     VEESPTLSASLIRGSKVDERGWAINDVVIRAAMQMVDVELYLDGQFVNTYPGDGMIVS
                     TPQGSTAYCMAAGGPVLGAGVRGFAIVPICAHSPIRTTLVAPEDALIELVLASEKAAD
                     LILDGVPAISLRDGDLVQVRRGTHRFRLVILPGMNFYQAFRSKFNFMIRPDAIPSRAA
                     QRAQPR"
     gene            4836..>5205
                     /locus_tag="VFU72_02925"
     CDS             4836..>5205
                     /locus_tag="VFU72_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU5422470.1"
                     /translation="MHWIEEAGLIAVVRLPDLGRAVRLADALADGGVRLLEFTYTNRE
                     AGAAIAEVRRALGERCRVGAGSVLDAETARQAILSGAEFIVTPTLRLATIELCRRYAV
                     PSVSGAFTPTEILTAWESGAD"
BASE COUNT          857 a         1751 c         1783 g          814 t
ORIGIN      
        1 ctccaacgtg gccgagcggc tgccgtcgcg tgtaccggcc catccgccgg cgccggcggg
       61 aacgaacatc cagctctgat cagtactccc gtcggtcacc ggcggacggc aatctgtgcg
      121 aacatcgcgt gtagaggcga aaacaaggag agaacagggc ctttgagcac accagctacc
      181 gaaagcacga ttcttcccgg atccgccccg ctcgtcggac aagagatcac cgacaacctg
      241 gaagacctgc tcgccgtgct gccgccgcat atcaccaagg ggattcgcgc gctgcacacg
      301 gcggagacgc gtagcgacct catcgagatc gtgctcgacc tgggccgtcg cccagaagct
      361 cgcttcaccg accgtgaact ctatctctcc gacgacgagg tcacgcgcga ggacatcgcc
      421 ttcgtggccg aacgcatcgg ccgcttcggc gacgacaacc gggccggcat agagcgcacg
      481 ctccatcgca tctcggccat ccgcagccgc tctggcgaga tcgtggggtt gacctgccgc
      541 gtcggccgcg ccgtctacgg cacgatcggg atcatccgcg atctggtcga ttccggggcc
      601 agcattctgc tcctcggccg tcccggtgtc ggcaagacga ccatgctgcg cgagaccgcg
      661 cgcgtgctgg ccgacgacct gcgcaagcgc gtggtggtgg tcgataccag caacgagatc
      721 gcgggggacg gcgacattcc ccacccctcg atcggccgcg cgcggcgcat gcaggtgccc
      781 acgcccgccc tacagcacgc ggtgatgatc gaggcggtcg aaaaccacat gccggaggtc
      841 atcgtcatcg acgagatcgg caccgagctg gaggccgcgg ccgcacgcac catcgccgag
      901 cgcggggtgc aactgatcgg caccgcgcac ggcaacacgc tcgagaacct gatgatgaac
      961 ccgaccctct ccgacctgat cggcggcatc cagtcggtga cgctcagcga cgaggaggcg
     1021 cgccggcgtg gcacgcagaa gtcgattctg gagcgcaagg cgcccccgac ctttgccgtg
     1081 atggtggaga ttcagacgcg cgaccgggtg gcggtccacc gcgacgtggc tcagagtgtc
     1141 gacgccatcc tgcgcggcca aacgctgcgc gtcgaggtac gcgagcggca gccaaacggc
     1201 gaggtgcgcg tctggcagga gcagccctac gaagccgagc acccaacgct ggtgcgccag
     1261 aacagtcgca atcggcagcc ggcggggtcg tcgaacgaga tcgcacccgc cgaggcaagg
     1321 ccgctggcgc cgcgccgccc gcccaagagc atccggatct tcccgttcgg cgtcagccgc
     1381 aaccggctgg aggagtcgat cagccagctg gcggtgccgg ccacggtcgt gcgtgagctg
     1441 cgcgaggccg acgccgtcgt gacgctgaag aactattatc gccgccagcc gcaccccctg
     1501 cgagatgccg aggcgcgcgg gctgccggtc tacgtgctgc gcaacaatac ggtggcccag
     1561 atcggcgcgc tgctccgttc gcttttccag gagagcgcgc agggcgagcc gcgggcgccg
     1621 caggggccct actccgacgt cgatcccaac cggtcgcccg aggaccaggt gaccgcggcc
     1681 atgttcgagg cagaggacgc catccaccac gtgatggacg gcgccacctc ggtcgagcta
     1741 cgaccccagg cggcctgggt gcggcgcctg cagcacgagc tggccgagcg ctacaacgtg
     1801 ccgtcgcgta gccgggggcg cgagccgcag cgctacgtcg aattcttccg tcgatgagcc
     1861 tcttcgtgac gctggagggg cccgagggcg gggggaagtc gacggtcggc ggccggctgc
     1921 gcgaggcgct tgaggcacgg ggcaagagcg tggtgctgac ccgtgagccg ggtgggacgc
     1981 cgctgggcga ggccgtgcgc gacttgctcc ttggtgcccg tcattctact atgcagcctg
     2041 aggtggaggc attgctcaac agcgcggcac gcgcccagca cgtggccgag gtcatcgcgc
     2101 cggccctgga cgaaggcaag atcgtgatct cggatcgctt tgccgattca accctggcct
     2161 atcagggcgc gggccgtggg ctcgatctca acgcgctggc cggcgtccag cggttcgcga
     2221 cccggggact gactcccgaa ctgacgctcc tgttcgatgt cccctacgaa gtcggccagg
     2281 ctcgtcgtcg tgagtcgggc gagttgaatc gcttcgacac cgatagtcgt gccttccacg
     2341 agcgcgtacg cgcctatttc ttgtgggccg cgcggcggga gccggagcgc tggcgcgtca
     2401 tcgacgccgc cgcgccgctc gacgaggtgg ccgagcaggc gctgcgattg gtgctcgatc
     2461 acctcgatgg ccagtcgatg agagtggggt agcagagcgg tgaagctcgt ttttgccgtc
     2521 atccaggggc gtgacgccga taatctgctg gccgcgctcc gcgacgcggg ctaccgctcg
     2581 acgctggtca gcagcgcggg cggattcttg cgagagcaga acgtgacggt gctgatcggc
     2641 gtggaagatc agtatgtgcc agatgtgatg cgcttgatca aacagaactg ctatacccgg
     2701 acgcgctacg tgaacccatt gctcccgatc atggagccgg gcgagttcta cgtgccggac
     2761 ccggtcgagg tgcaggtggg tggtgccacc gtgttcgtgc tcaatgtacg ctatgttgaa
     2821 cgcgtgatct agtctgggcg cggtgggcac ggaggcccga gaggggaaga tgaaatggca
     2881 gcgctgatcg cgcgcggcgt gcagctcttg atcgcactgg gggtcgccta tttcctggcg
     2941 ctctggttcg ccttgatcgt ctggacgttc caggatatcc agtcacgcag ccgcagcgtc
     3001 atcgcgcaga tcttctcgac gctggtcgtc gtgctcttct ctttccccgg agtgatcctc
     3061 tacctgatcc tgcgcccgcg cgacaccctc gacgacgcct tccagcgttc gctggaagag
     3121 gaatacctct tgcaggacct ggaagagctg ccgctctgtc ccacttgcca gcactacgtg
     3181 gagcaggact acgtcttctg cccacactgt cgaaccgagc tgaaggagcc gtgcgccggc
     3241 tgtgggcgtc ccatcgacat tcgctgggag atctgcccct tctgcggcga ggagcagttc
     3301 gaggaggagc tgccggccgc ccgcctggcc gaggagtggg agcccgccga ggtcgatccg
     3361 gtcacgcgcc tgctggggca ggcccgcgag cggctctcgg gccggcgcac gcgtgagctc
     3421 ggcgaggggg tgggtgcgga cgagcgtggg atggccgagc tgcagcaggg cgggcagagc
     3481 tatcttccct gggagagcga ggcacccacc gtcgacacct gggcgcgccg gcccgagccg
     3541 aacgcgttgc gcggtgagcg cgaaccgcgt tggggcgacg acggcgccgg gccgggcgag
     3601 ggcgacacgt acgaccccac cgaggagatc ccgatcgcgc cacctccacg gccgtaggga
     3661 cgcctttcga cttcattggg tgtttggtgg agacggatcc ttcgacacgc tcaggatgac
     3721 agggtcaagg gatgcggcgt acgagtacgt cacgtcgtat tcgtcggctc cgtcttagcc
     3781 cattatcctg agctgagtcg aagattccgt cctcacccgc cacccacccc gatcgatgga
     3841 cgcctgtggt ccttcagcgc ggctgcgcgc gctgcgcggc gcgcgagggg atggcgtcgg
     3901 gacggatcat gaagttgaac ttgctgcgga acgcctggta gaagttcatg ccgggcagga
     3961 tcaccaggcg gaagcggtgc gtaccgcggc ggacctgcac cagatcgccg tcgcgcaggc
     4021 tgatcgccgg cacgccatcg agaatcaggt cggcggcttt ctccgaagcc agcaccagct
     4081 cgatcagcgc gtcctccggc gcgaccagcg tggtgcggat cggcgaatgg gcgcagatcg
     4141 gcacgatggc gaagccgcgc acgccggccc ccaatacggg tccgccggcc gccatgcagt
     4201 aggcggtgct cccctgtggc gtcgagacga tcatgccatc accagggtag gtgttgacga
     4261 actggccgtc cagatagagc tcgacgtcga ccatctgcat cgcggcgcgg atcaccacgt
     4321 cattgatcgc ccagccgcgc tcatcgacct tgctcccccg gatcaagctg gccgagagcg
     4381 tcgggctctc ctccacccgg tagcgcccct cgacgacttc ggacagggct ggacgccagg
     4441 cgctgcgctc gatgcgcgcc aggaagccga ccttgcccag gttgatgccc aggatcggca
     4501 cgtcgggcac gttgtgcgcc aagcgcataa tcaggccgtc tccgccgatc gtcagcagga
     4561 gatcggccga atcaccgcgc agcagcgcgt cctccgatac gaccgcacaa cccgccgcct
     4621 gcagccaggg catcagctcc tcggccagct ccgccgcctc cggcttgctg cgcgcgggaa
     4681 tcacccctat ccgtagaccc accgaacccc ctcgccgctg cgcgtccccc aattgacttg
     4741 cccgaatcct accgcacgcc tgtcgcgcgg gcgccggatc cacgcgctag aaggtacact
     4801 ttcagtgaga attgggcgcg ggagagacgg ttatcatgca ctggatcgaa gaagcgggac
     4861 tgatcgcggt tgtgcgcctg ccggacctcg gccgggcggt gcggctggcc gacgcgttgg
     4921 cggacggtgg ggtgcgcctg ctggagttca cctacaccaa ccgcgaagcc ggggcggcca
     4981 tcgccgaggt gcgccgcgcg ctgggcgaac gctgccgggt cggcgccggc tcggtgctgg
     5041 acgccgagac ggcgcggcag gcgatcctga gcggcgccga attcatcgtg acaccgaccc
     5101 tgcggctcgc gacgatcgag ctctgccgtc gctacgccgt gccctcggtc agcggcgcct
     5161 tcacaccgac cgagatcctg accgcctggg agtccggcgc tgact
//
LOCUS       DASUBI010000156         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1167867, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000156 DASUBI010000000
VERSION     DASUBI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167867"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFU72_02930"
     CDS             <1..303
                     /locus_tag="VFU72_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5422471.1"
                     /translation="VEEFPTITFKSTRVEPKGKDHARVYGDLTVRGTTREVELDTEIN
                     GVGKNPYGQEVVGFSAETSISRKEWGLTWNVALESGGWLVADRLKITIEGQATKQE"
     gene            713..>2536
                     /locus_tag="VFU72_02935"
     CDS             713..>2536
                     /locus_tag="VFU72_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5422472.1"
                     /translation="MGAENEIASLAHWRLVGPFRGGRVVAVAGDPVDPLTGYFGACAG
                     GIWKTEDAGQSWRNVSDGYLKTASVGALAVAPSDRNVVYAGMGEACIRGNVSHGDGVY
                     RSDDAGKSWRHLGLADTRHIGRVRVHPHNPDLVYVAALGHAWGPNAERGVFRSRDSGQ
                     SWEKILSRDEKTGAIDLTMDPNNPRVLYAALWEAQRYPWALVSGGPGSGLFKSSDGGD
                     SWTELTNNPGLPEGLKGRIGVAVSPARPERVWAIIEAKEGGLYRSDDGGARWELVNDN
                     PDLRQRPWYYMHLFADPGDAETVWVLNLRMWKSTDGGKSVMQVPTPHGDNHDLWIDPE
                     NSRRMLTGNDGGACVSLDGGVTWSSIYNQPTAQFYHVITDDQFPYRIYGAQQDNSTLS
                     VPSYSDKGAIVAGDTYPVGGGESGYIAVRPDNPNIVFAGSYASRMTRYNHASANEVDI
                     TVWPEDPIGYGAGSLRYRFQWTFPIILSPHDPNLLYAAGNHVFRSTDGGQSWQELSSD
                     LTRAEPETLKPSGGPITLDNVSTEYYGTIFALAESPVHKGVIWAGSDDGLIHVTRDGG
                     QSWQNVTPPRALVGEWALINIIEASPHDAATAYVAATRYKLD"
BASE COUNT          440 a          915 c          827 g          354 t
ORIGIN      
        1 gtcgaggagt ttcccaccat caccttcaag agcacgcgcg tggagccaaa gggcaaggat
       61 cacgccaggg tctacggcga tctgacggtg cgcgggacca cgcgcgaggt cgagttggac
      121 accgagatca acggcgtggg caagaacccc tacggccagg aggtcgtcgg cttcagcgcc
      181 gagacgagca tcagccgcaa ggagtggggc ctgacctgga acgtggccct ggagtccggc
      241 ggctggctgg tggccgaccg tctgaagatc accatcgagg gccaggcgac caagcaggag
      301 taacgagccc tcacccctac ccctctccca atgctgggag aggggaattt ttgtgtgtgg
      361 agggtttgcc atcgatcggg gagctcgtcg ccgacggctc gcgcggggtt gaaaccgccg
      421 cgctaataag cgaagcctgc tcaagcaggc tgggaacgtt ccaaactccc agccttcttg
      481 agcaggcttt cgtcaagatc tagcgcggtg gttttaaccc cgcgcgccga gcggcactca
      541 gccgccacgg accacgccaa accctccacg actctcccgc gccacgcgcc acctaaactc
      601 cccgaacttc accgcctcac gccttgcccc tgccccgctc ggcgctatca tggccgggac
      661 gcggcgcgtc aggcgcggcg cgggcatacg gagctatggt ggaggaggga acgtgggcgc
      721 ggagaacgag atcgcgagcc tggcgcactg gcgcctggtg gggccgtttc gcggcgggcg
      781 cgtggtggcg gtggccggtg atcccgtcga tccgctcacc ggctacttcg gcgcctgcgc
      841 cgggggcatc tggaagaccg aggacgccgg gcagtcgtgg cgcaacgtct cggacggcta
      901 cctcaagacc gcttcggtcg gcgcgctggc cgtcgctccc tccgaccgca acgtggtcta
      961 cgccggcatg ggcgaggcct gcatccgggg caacgtctcg cacggcgacg gcgtctaccg
     1021 ctccgacgac gccggcaaga gctggcggca cctcggcctg gccgacacgc gccacatcgg
     1081 ccgcgtgcgc gtccatcccc acaatcccga cctggtctac gtggccgcgc tggggcacgc
     1141 ctggggaccg aacgccgagc gcggcgtctt ccgctccaga gacagcggcc agagttggga
     1201 gaagatcctc tcccgcgatg agaagaccgg cgccatcgac ctgaccatgg accccaacaa
     1261 cccgcgcgtc ctctacgccg cgctctggga ggcgcagcgc tacccctggg cgctggtcag
     1321 tggcggcccc ggcagcggcc tcttcaagtc gagcgacggc ggcgacagtt ggaccgagct
     1381 gaccaacaac cccggcctgc ccgaggggct gaagggccgc atcggggtgg ccgtgtcgcc
     1441 ggcccggccc gagcgcgtct gggccatcat cgaggccaag gaagggggac tctaccgctc
     1501 cgacgacggc ggcgcccgct gggagctggt caacgacaac cccgacctgc gccagcgacc
     1561 ctggtactac atgcacctct tcgcggaccc cggggacgcc gagaccgtct gggtgctcaa
     1621 cctgcgcatg tggaagtcga ccgacggcgg caagtcagtg atgcaggtgc ccacgccgca
     1681 cggcgacaac cacgatctct ggatcgaccc ggagaactcg cgccgtatgc taaccggcaa
     1741 cgacggcggc gcctgcgtct cgctcgacgg cggcgtcacc tggtcgagca tctacaacca
     1801 gccgacggcg cagttctacc acgtcatcac cgacgaccag tttccctatc gcatctacgg
     1861 cgcgcagcag gacaacagca ccctctcggt gcccagctac tccgacaagg gcgcgatcgt
     1921 ggctggcgac acctacccgg tgggcggcgg cgagagcggc tatatcgcgg tgcggccgga
     1981 caaccccaac atcgtcttcg ccggcagcta cgcctcgcgc atgacgcgct acaaccacgc
     2041 cagcgccaac gaggtcgaca tcaccgtctg gcccgaggac ccgatcggct acggcgccgg
     2101 gtcgctgcgc taccgcttcc agtggacctt cccgatcatc ctctcgccgc acgacccgaa
     2161 cctgctctac gccgccggca accacgtctt ccgctcgacc gacggcgggc agagctggca
     2221 ggagctgagc tccgacctga cgcgcgctga gccggagacg ctcaagccct ccggcggccc
     2281 gatcacgctc gacaacgtca gcaccgagta ctacggcacc atcttcgccc tggccgagtc
     2341 gccggtgcat aagggtgtca tctgggctgg ctccgacgac ggcctgatcc acgtcacgcg
     2401 cgacggcggg cagagctggc agaacgtcac cccgccgcgc gcgctggtcg gcgagtgggc
     2461 gctgatcaac atcatcgaag cctcgcccca cgatgccgcc accgcctatg tcgccgccac
     2521 gcgctacaag ctcgac
//
LOCUS       DASUBI010000157         4308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1045165, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000157 DASUBI010000000
VERSION     DASUBI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045165"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="rsmH"
                     /locus_tag="VFU72_02940"
     CDS             <1..920
                     /gene="rsmH"
                     /locus_tag="VFU72_02940"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5422473.1"
                     /translation="PVLPREMLEYLAPRSGGRYIDGTFGGGGHARAILEASAPEGRLL
                     ALDADPAAVARARELAARYPGRLTAAPGNFRDIVPLARAAGFEPVEGVLLDLGVSSFQ
                     LDQAERGFSFRFSGPLDMRFNPDFGSSAADIVNGSTPEELARVLFEFGEEPNARRIAR
                     AIERERRQAPITTTARLAVIVERAVGGRRGRPIHPATRTFQALRIAVNEELDALRQGL
                     AGAIALLAPGGRIVAISFHSLEDRIVKSVFRREASGCVCPPGTRVCVCGHQPQLRLLT
                     RRPVEPAPAEAVANPRSRSAKLRAAERLP"
     gene            917..1450
                     /locus_tag="VFU72_02945"
     CDS             917..1450
                     /locus_tag="VFU72_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422474.1"
                     /translation="MTLTVRRPRLIDRTEPIELVEQVEPAARARPRRAGRGYLMLNGA
                     ALVAIAALFLGCIGILYLIQTSQVAQLGYQMSHLQTRYDELTDANEKLRYQIDRDHSL
                     PVVQQIAITKLEMAPLHTFKFLQVQRPEQDQLPPPAPEPRTHLSLWQQMREAILGTSR
                     ADHLDSPPAASRAGAQP"
     gene            1447..3168
                     /locus_tag="VFU72_02950"
     CDS             1447..3168
                     /locus_tag="VFU72_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5422475.1"
                     /translation="MNQNQFRIPRRRIYVLFIGFVIFSLAISYRIVTFQVVHSQQLSQ
                     EAVASRLREDVVPARRGEILDAEGRPLATNIPADQLAAIVQQVKDPKQTAAALAPIIG
                     RSAEDIEAAITAPGKEWVVLKTRLSPEASQQITDLKLPGLVLSPEPRRIYPNDDFASQ
                     VLGFVNYNNDGSYGIEGKYNNLLSGTPGKLIGERDEQGNVIALANSVYDPPKDGADLV
                     LTIDSSIQWIVEQALDQAMKDQQASGGTIIVENPKTGAILAMASRGSFDPNAFETVED
                     PSVFVNPAISDIYEPGSTFKPLGMSLALDQGLVSPDTTYDGGSYRILPGGAKVTNALG
                     AAFGPETMTQVLEHSSNLGMMHVADLIGEQRFYQGLEAFGIGQPTGVDLQGESGGILP
                     LPGEPDWSAANFYTNTFGQGLAVTPLQLLNAICVLANGGELMQPYVVQQIRNADGTVK
                     QIQPQVEHRVISPQTSRTITDMLTAVMKTTYTRFAIPGYDVAAKTGTAQIPSPNGGYD
                     PNSTIGSVIGYGPSSDPQFAVLVKIDRPQNADWGEEAAGPAFKQVMQQLFLLKGIPPT
                     EATGTAP"
     gene            3200..>4308
                     /gene="murF"
                     /locus_tag="VFU72_02955"
     CDS             3200..>4308
                     /gene="murF"
                     /locus_tag="VFU72_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5422476.1"
                     /translation="MVSKLAPVPGDDGPPAVDPERAGERGSGLIALAQVLEGTVGRLH
                     GTAPAGLAFRRVWHDSRQVGPGDLFVALKGESLDGHDFVAQAFARGASAALVDRAHRD
                     ALAPLGRPLIVVRDTLAALHDLATYWRGLYATTVIGITGSIGKSSTKEVVAAVAARRY
                     RTVRNRGSFNNEVGLPLTLLDIRPDTEVAVLEMGGAYAFGEIAELCVMARPRIGIVTN
                     VSHSHLSRMGSLDAIARTKVELPAALPPEGAAILNGDDPRVRAMAAACRCRVLFYGLS
                     DDCDVRALDVQGHGLNGISFRLVLDGRVEHVKLPLLGRHSVHTALAGIAAGSVLGLSI
                     DEILPGFDDPAIQLRLYTLPGVGGALVIDDSYNANP"
BASE COUNT          684 a         1529 c         1460 g          635 t
ORIGIN      
        1 agccggtgct gccgcgtgag atgctggagt atctggcgcc gcggtccggc ggccgctaca
       61 tcgacggcac cttcggcggc ggcgggcacg cgcgggcgat cctggaggcc agcgcgccgg
      121 aggggcggct gctggcgctc gacgccgacc ccgccgccgt ggcgcgcgcc cgcgagctgg
      181 ccgcgcgcta tcctggccgg ctgacggccg cgccgggcaa cttccgcgac atcgtgccgc
      241 tggcgcgcgc cgccggcttc gagccggtcg agggcgtgct cctcgatctg ggggtctcct
      301 cgttccagct cgatcaggcc gagcgtggct tctccttccg cttcagcggg ccgctcgaca
      361 tgcgtttcaa ccccgatttc gggtcgagcg ccgccgatat cgtgaacggc tccacgccgg
      421 aggagctggc gcgcgtactc ttcgagttcg gcgaggagcc aaacgcacgg cgcattgcgc
      481 gggcgatcga gcgcgagcgg cggcaggcgc cgatcaccac gaccgctcgg ctggcggtca
      541 tcgtcgagcg agccgtgggc gggcgacgcg gcaggccgat ccaccccgcc acacgcacct
      601 ttcaggcgct gcggatcgcg gtcaatgaag aactggacgc gctgcgtcag gggctggccg
      661 gagccatcgc gctgctggcc ccgggcgggc gtatcgtggc catctccttc cactcgctgg
      721 aggaccgtat cgtcaagagc gtcttccgac gcgaggccag cggctgcgtc tgcccgccgg
      781 gcacgcgcgt ctgcgtctgc ggccaccagc cgcaactgcg tctcctgacg cgccgcccgg
      841 tcgagccggc gcccgccgaa gcggtggcca acccccgtag ccgcagcgcc aagctccgcg
      901 cggcggagcg gctgccatga cgctgaccgt gcgccgcccg cgactgatcg atcgcaccga
      961 gccgattgag ctggtcgagc aggtcgagcc ggctgcacgg gcgcggccgc gccgcgccgg
     1021 gcgcggctac ctgatgctca acggcgcggc tctggtggcg atcgcggcgc tcttcctggg
     1081 ctgcatcggg atcctctatc tgatccagac cagccaggtg gcccagctcg gctaccagat
     1141 gagccacctc caaactcgtt acgacgagct gaccgacgcc aacgagaagc tgcgttatca
     1201 gatcgaccgc gatcactcgc tgccggtcgt gcagcagatc gccatcacca agctggagat
     1261 ggctccgctg cacaccttca agttcctcca ggtgcagcga ccggagcagg atcaactccc
     1321 gccgccggcg ccggagccgc gcacgcacct ctcgctctgg cagcagatgc gcgaggcgat
     1381 cctcggcacc agccgcgccg atcacctcga cagcccgccg gcagcgagcc gcgccggagc
     1441 ccagccgtga accagaacca gttccgcatc ccgcgccgtc gcatctatgt tctcttcatc
     1501 ggcttcgtca tcttctcgct ggcgatcagc tatcgcatcg tcaccttcca ggtggtccac
     1561 agccagcagc tttcgcagga ggcggtcgcc tcgcgactgc gcgaggacgt cgtcccggcg
     1621 cggcgcggcg agatcctgga cgccgagggc cggccgctgg ccaccaacat cccggccgac
     1681 caactggcgg cgatcgtgca gcaggtgaaa gacccgaagc agacagcggc ggcgctagcg
     1741 ccgatcatcg ggcgtagcgc ggaggacatc gaggcggcca tcaccgcgcc gggcaaggag
     1801 tgggtggtgc tcaagacccg cctctcgccc gaggcctcgc agcagatcac tgacctgaag
     1861 ctcccggggc tggtgctcag ccctgagccg cgccgcatct accccaacga cgacttcgcc
     1921 tcgcaggtgc tcggtttcgt caactacaac aacgacggct cctatggtat cgaggggaag
     1981 tacaacaacc tgctctcggg cacaccgggc aagctgatcg gcgagcgcga tgagcagggg
     2041 aacgtcatcg cgctggccaa tagcgtctac gacccgccca aggacggcgc cgatctggtg
     2101 ctgacgatcg atagctcgat ccagtggatc gtcgagcagg cgctcgatca ggcgatgaaa
     2161 gatcagcagg cgtcgggtgg caccattatt gtagagaatc ccaagactgg cgccatcctg
     2221 gcgatggcca gccgcggctc gttcgacccc aacgccttcg agacggtgga agatccgagc
     2281 gttttcgtca atccggcgat ctcggacatc tacgagcccg gctcgacctt caagccgctc
     2341 gggatgtcgc tcgcgctcga ccagggcctg gtgtcgcccg acacgaccta cgacggcggc
     2401 tcgtaccgca tcctgccagg cggcgcgaaa gtcaccaacg cgctcggcgc cgccttcggc
     2461 cccgagacga tgacgcaggt gctggagcac tcgagcaact tgggcatgat gcacgtggcc
     2521 gatctgatcg gtgagcagcg cttctatcag ggtttggaag ccttcggcat cgggcaaccg
     2581 accggcgtcg acctgcaggg cgagtccggc ggcatcctgc cgctgccggg cgagccggac
     2641 tggagcgccg ccaatttcta caccaacacg ttcgggcagg ggctggcggt gacgccgcta
     2701 cagctgctca acgctatctg cgtgctggcc aacggagggg aactgatgca gccctacgtg
     2761 gtgcagcaga tccgcaacgc cgacggcacg gtcaagcaga tccagccgca ggtggagcac
     2821 cgcgttatct cgccgcagac ctcgcgcacg atcaccgaca tgttgacggc cgtgatgaag
     2881 acgacgtaca cgcgcttcgc catccccggc tacgacgtgg ccgccaagac cggcacggcg
     2941 cagatccctt cgcccaatgg cggctacgac cccaacagca ccatcggctc ggtgataggc
     3001 tacggcccgt cgtccgaccc gcagttcgcc gtgctggtca aaatcgaccg cccgcagaac
     3061 gccgactggg gcgaggaagc ggccgggcca gccttcaagc aggtgatgca gcagctcttc
     3121 ctgctgaaag gcatcccgcc gaccgaggcc accggcaccg cgccgtagga cgagcgattc
     3181 tataagggaa gagggagtca tggtttcaaa gctcgcgccc gtacccggcg acgacggccc
     3241 gccggccgtc gacccggagc gcgccggcga gcgtgggagc ggcctgatcg cgctggccca
     3301 ggtgctggag gggaccgtcg ggcggctgca cggcacggcg ccggcgggtc tcgccttccg
     3361 gcgcgtctgg cacgactcgc ggcaggtcgg ccccggcgat ctcttcgtgg ccctgaaggg
     3421 ggagagcctc gacggccacg acttcgtggc gcaggccttc gcgcgtggcg ccagtgcggc
     3481 gctggtcgac cgcgcgcacc gtgacgcgct ggcgccgctg ggccggccgc tgatcgtggt
     3541 gcgcgacacg ctggccgcgc tgcacgacct ggcgacctac tggcgcgggc tctacgccac
     3601 aaccgtgatc ggtatcaccg gcagcatcgg caagtcgagc accaaggaag tggttgcggc
     3661 ggtggccgcg cggcgctacc gcacggtgcg caatcgcggc agcttcaaca acgaggtcgg
     3721 cctgccgctg acgctgctgg atatccggcc cgacaccgaa gtcgcggtgc tggagatggg
     3781 cggcgcctac gccttcggcg agatcgccga gctgtgcgtc atggcccggc cgcgcatcgg
     3841 cattgtgacc aacgtctcgc actcgcacct ctcgcgcatg ggctcgctgg acgccatcgc
     3901 gcgcaccaag gtggagctgc ccgccgcctt accgccggaa ggggctgcca ttctgaacgg
     3961 cgacgacccg cgggtgcgcg cgatggcggc ggcctgccgc tgccgggtgc tcttctacgg
     4021 attgtcggac gactgcgacg tgcgcgcgct cgacgtgcag ggtcacgggc tgaacggcat
     4081 ctcctttcgt ctggtgctcg acggccgcgt ggagcacgtc aagctgccac tgctcgggcg
     4141 ccacagcgtc cacaccgcgc tggccggcat cgcggccggt tcggtcctcg gactgagcat
     4201 cgacgagatc ctgccgggct tcgacgatcc ggcgatccaa ctgcggctct acacgctgcc
     4261 cggcgtcggc ggcgcgctgg tcatcgacga cagctacaac gccaaccc
//
LOCUS       DASUBI010000158         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1235765, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000158 DASUBI010000000
VERSION     DASUBI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235765"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFU72_02960"
     CDS             <1..483
                     /locus_tag="VFU72_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422477.1"
                     /translation="FVTEHVEEFGNDGVVWMVDYSQTNRLLPTQLKSVLQDEGSSVFT
                     AEMLERVTPSLRQFTQLSDDDYVLFFEPPSLDERIVNQSALFSLMANPRSRLDGWLER
                     HPEVYRRLLIPVELKWEIRDKLDQANITERVLYPGLDGLSRYLRRYYTPNAHRQKRQR
                     "
     gene            complement(731..1600)
                     /locus_tag="VFU72_02965"
     CDS             complement(731..1600)
                     /locus_tag="VFU72_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5422478.1"
                     /translation="MIETIVVPLDGSPLAAEALPTARAIAGRTGKPLRLVRVIAPSAP
                     AAARDEAESYLKTTAEQVGGAETAVWLGDPAEQIIDGLSKLPDAVVVMTTHGRTGIGR
                     WLFGSVADRVVHAGVAPVLLIRSGNPAPTILRQIMVPLDGSALSETSLPMAEELAHDF
                     AAKLQLVRVVETTSLYGMFGADYGAIAAAPEALQQLLDEMDADAHSYLDGVAAKLKER
                     GLTADTTVLEGLIADQLLAFGRQQRADLIVMATRGRAGVSRLVLGSVAEQMLRLGDRP
                     LLLVPPQAPEPAE"
     gene            complement(1780..>4215)
                     /gene="mgtA"
                     /locus_tag="VFU72_02970"
     CDS             complement(1780..>4215)
                     /gene="mgtA"
                     /locus_tag="VFU72_02970"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEU5422479.1"
                     /translation="IRRRVAMGCNEVSATTGASVAMQLLGFIANPLVAILLIASVVSA
                     VLGEVANAVIITVIVVLSVVLDFVQSFRSQRAAERLRASVAPLATALRDGRWITLAPR
                     ELVEGDIIALKAGDLVPADARLLESRDLHVQQAALTGEALPVEKRVLERPSPRSAGLD
                     APDLIFLGSSVISGTAMTEVVATGERTTFGDVVARLRRHPPATEFERGLRDFGYLIMR
                     TVFLLVLFVLLVNVALRRDPLESLLFALALAVGLTPEFLPMITTVTLGQGALHMERQK
                     VIVKKLAAIQNFGSIDVLCSDKTGTLTSGRMALEQSLDPAGSPSPLPLELATLNSRFQ
                     TGIRSPLDDAVLAAGPAGDGDVSKIDEIPFDFERRRLSVVLDVGGVRRLITKGAPESV
                     LPCCTSYQSQGEIRPLDPSTRGQLAALHQSLAQQGYRVLAVACAAVPRRATYTPADEA
                     GLTLAGFLAFSDPLLPDAAEALATLRADGVRVMVLTGDNEAVARHACAAAGIDAGRIM
                     LGEELERRDDRQLQQLVEDVSVFARVTPGQKDRVILALKRAGHVVGYLGDGINDAPSL
                     HTADVGISVVNGTDVARDVADIILLEPGLRVLQSGIIEGRKAFGNVVKYLLMGTSSNF
                     GNMFSMAAATLFLPFLPMLPTQILLNNFLYDLAQITIPTDTVDPMFIRQPHRWDIRLI
                     RNFMLLIGPISSLYDFLTFFVLLKIFDASAELFHTGWFIESLVTQTLVLLVIRTAGTP
                     WRSRPSTTLALTLLAVILVAIALPFTPLAGTLGFVALPATYFLFLLLATLTYLALVEV
                     VKRPLLRRYWGRA"
BASE COUNT          722 a         1464 c         1409 g          620 t
ORIGIN      
        1 ttcgtcaccg agcatgtgga ggagttcggc aacgacggtg tggtctggat ggtcgactat
       61 tcccagacca accggctcct gcccacccag ctcaagagcg tgctgcagga cgagggctcc
      121 tcggtcttca ccgccgagat gctggagcgc gtcacgccca gcctgcgcca gttcacccag
      181 ctctccgatg acgactacgt cctcttcttc gagccgccct cgctcgacga gcgcatcgtc
      241 aaccagtcgg cgctcttctc gctgatggcc aacccgcgca gccggctcga cggctggctg
      301 gagcggcacc ccgaggtcta ccggcgcctg ctgattcccg tggagctgaa gtgggagatc
      361 cgcgacaagc tcgatcaggc caacatcacc gagcgggtgc tctaccccgg cctggacggc
      421 ctctcccgct acctgcgccg ctactacacc cccaacgccc accgccagaa gcggcagcgg
      481 tgaggggcca gggtggtgcc catcgacgat gttgcctgcc gctctacgac ggatccttcg
      541 acttcgctca gagcctgccc tgagcctgcc gaagggatga cagggctaag gacggggcat
      601 acgacgccga ctcgtgccgt ccgaaaggcc acgaaccaaa cccggtcatc ctgagcttga
      661 tcctgagcct gccgaaggaa ggatccgtcc tcacaagcgc cccatcgttg tctttagggg
      721 cgaccgtccg ctactccgcc ggctccggcg cctgcggtgg caccagcagc agcgggcgat
      781 cgcccagccg cagcatctgc tcggccacgc tgcccagcac caggcggctg acgccggcgc
      841 gcccgcgcgt cgccatcacg atcagatcgg cccgctgctg ccgtccgaag gcgagcagtt
      901 gatcggcgat cagcccctcc agcaccgtcg tatcggccgt caggccgcgc tctttcagct
      961 tggcggccac accgtccagg tagctgtggg cgtctgcgtc catctcgtcc agcaactgct
     1021 gcagcgcctc gggcgcggcg gcgatcgccc cgtagtcggc gccgaacatg ccgtagaggg
     1081 aggtcgtctc gaccacgcgc accaactgga gtttcgcggc gaagtcgtgc gccagctcct
     1141 ccgccatcgg cagggacgtc tcgctgagcg ccgagccgtc gagcgggacc atgatctgcc
     1201 gcaggatggt cggcgccgga ttgccggagc gaatcagcag gaccggcgct accccggcgt
     1261 gcaccacccg gtcggccacg ctgccgaaga gccagcggcc gatgccggtg cgaccgtggg
     1321 tggtcatcac gaccaccgcg tccggcagct tcgacaggcc gtcgatgatc tgctcggccg
     1381 gatcgcccag ccagacggcc gtctccgcgc cgccgacctg ctcggcggtc gtcttcaggt
     1441 agctctcggc ctcgtcgcgg gccgcggcgg gcgcgctggg cgcgatcacg cgcaccagcc
     1501 gcagcggctt gccggttcgt ccggcgatcg ccctggcggt gggcagcgcc tccgcggcca
     1561 gcggcgagcc atcgagcggc acgacaatgg tctcaatcac ggactgctcc tcctcatcca
     1621 cgctcatgac acgcggcgcg aacgagctcg cgcttgggcg taagaccggc gcatgtccgg
     1681 cggcgtcgcc ggagcatggc agcgacggcc tcgatgtcac aagcatgtga taagagcgtc
     1741 cgggccgcaa cagtctgctg gccggacgct ccgatcggat caggcgcgtc cccagtagcg
     1801 acggagcagc gggcgcttga ccacctccac cagcgccagg taggtcagcg tcgccagcag
     1861 cagaaacagg aaataggttg cggggagcgc gacgaaaccg agcgtgccgg cgagcggcgt
     1921 gaacggcagc gcaatcgcga ccagaatcac cgccaacagc gtcaacgcca gcgtcgtgct
     1981 cggccggctg cgccagggag tgccggcggt gcggatcacc agcagcacga gcgtctgggt
     2041 caccagcgat tcgatgaacc agccggtgtg gaagagctcg gcagaggcgt cgaagatctt
     2101 cagcagcacg aagaaggtca ggaagtcata gagcgagctg atcggcccga tcagcagcat
     2161 gaaattccga atcaggcgga tgtcccagcg atgcggctgg cggatgaaca tcggatcgac
     2221 cgtatcggtc gggatcgtga tctgcgcgag gtcgtagagg aaattgttga gcaggatttg
     2281 ggtgggcagc atcggcagaa acggcaggaa gagtgtcgcc gccgccatgc tgaacatgtt
     2341 cccgaaattc gagctggttc ccatcaagag atacttgacg acgttgccga acgccttgcg
     2401 gccctcgatg atgccgctct gcagcacgcg caggcccggc tccagcagga tgatatcggc
     2461 gacgtccctg gccacgtcgg tcccattgac gaccgagatg ccgacatcgg cggtgtgcag
     2521 cgacggagcg tcgttgatcc cgtcgccgag gtagccgacg acatggccag cgcgcttgag
     2581 cgccaggatc acgcgatcct tctgccccgg cgtgacccgc gcgaatacgc tgacgtcctc
     2641 caccagttgt tgcagctggc gatcgtcgcg gcgctccaac tcttcgccca gcatgatccg
     2701 cccggcgtcg attccggccg ccgcgcaggc gtggcgggcc accgcctcgt tgtcgccggt
     2761 cagcaccatc acgcgcacgc catcggctct cagcgtcgcc agcgcttcgg cggcgtccgg
     2821 taacagcgga tcgctgaacg ccaggaagcc ggccagggtc agcccggcct cgtcggcggg
     2881 tgtgtacgtc gcccggcgcg gcacggccgc gcaggcgacc gccagtacgc gatacccctg
     2941 ctgggcgagc gactgatgca gcgctgccag ctggccgcgc gtgctgggat cgagcggccg
     3001 gatctccccc tgggactggt agctcgtgca gcagggcagc acgctctccg gcgcgccctt
     3061 ggtgatcagc cggcgaacgc cgcccacgtc gagcacgacc gagagccggc ggcgctcgaa
     3121 gtcgaacgga atctcgtcga tcttggagac atcgccatcg ccggccggcc cggccgctag
     3181 cacggcgtcg tcgagagggc tacggatgcc ggtctggaag cggctgttga gtgtcgccag
     3241 ctccagcggt agcggggacg ggctgccagc cggatcgagc gactgctcca gggccatccg
     3301 gccgctggtc agggtgccgg tcttgtcgct gcagaggacg tcgatgcttc cgaaattctg
     3361 gatcgccgcc agcttcttga cgatgacctt ctgccgctcc atatgcagcg cgccctggcc
     3421 gagcgtcacg gtggtgatca tcgggaggaa ttcgggcgtc agcccgaccg ccagcgccag
     3481 cgcgaacagc aacgactcga gcggatcccg ccgcaaggcg acgttcacca gcagcacgaa
     3541 gagcaccagc aggaagaccg tgcgcatgat caggtagccg aagtcccgca gaccgcgctc
     3601 gaactcggtc gcgggtggat ggcgccgcag ccgcgcgacg acatccccaa aggtggtgcg
     3661 ctctccggtc gccaccacct ccgtcatcgc ggtgccgctg atgaccgagc tgcccaggaa
     3721 gatcagatcg ggtgcatcca gcccggcgga gcgcggtgag gggcgctcca gcacgcgctt
     3781 ctccaccggc agcgcctcgc cggtcagggc cgcctgctgg acgtgcagat cgcgggactc
     3841 gagcagccgg gcgtcggcgg ggacgagatc gcccgccttc aacgcgatga tgtcaccctc
     3901 gaccagctcc cgcggcgcga gggtgatcca gcggccgtcg cgcagggcgg tcgccagcgg
     3961 tgcgaccgag gcgcgcagcc gctcggcggc gcgctgcgag cggaacgact gcacgaaatc
     4021 gagcaccacg ctgagcacga cgatgaccgt gatgatgacc gcgttggcga cctcacccag
     4081 caccgccgaa acgacgctgg cgatcagcag aatcgccacg agcggattgg cgatgaaacc
     4141 gagcaactgc atcgccaccg aggctccggt ggtggcgctg acctcgttgc aacccatagc
     4201 cacccggcgt cggat
//
LOCUS       DASUBI010000159         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_282430, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000159 DASUBI010000000
VERSION     DASUBI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282430"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFU72_02975"
     CDS             complement(<1..197)
                     /locus_tag="VFU72_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5422480.1"
                     /translation="MRIGVLATRAGVTAKTIRYYETFGLLPAPRRATNGYRCYTEADL
                     TRLRFIRRAQAAGLSLQAIGS"
     gene            complement(382..825)
                     /locus_tag="VFU72_02980"
     CDS             complement(382..825)
                     /locus_tag="VFU72_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5422481.1"
                     /translation="MPPHPQFGQPPADGICRDRPGVYAVALVAGRLLLVETPAGRFLP
                     GGGRETGESAEAALRRELREETGYQLAALRELGAAGQYVIERSTGICYNKLETFFLVE
                     LGARVDGTSEPDHTLRWLAPHDALTALHEEAQAWAVRRALRFSPQ"
     gene            complement(832..1125)
                     /locus_tag="VFU72_02985"
     CDS             complement(832..1125)
                     /locus_tag="VFU72_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422482.1"
                     /translation="MPRSLTWSQRERPLWLPPLVSVATLFGLFLTAMAVRRSSLATPL
                     RPLMVALSGLAAAAIALDGAALLLQARARGLAAAVSGLVMLALGVMTSRHVLR"
     gene            complement(1137..2447)
                     /locus_tag="VFU72_02990"
     CDS             complement(1137..2447)
                     /locus_tag="VFU72_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422483.1"
                     /translation="MSEREQAEQLDRALARRRAGGQSAEDEPELSAELRELAALAGRL
                     ERELPPELPDPAFRLRQELLGEPVITPLPRHWYQDWRVAVIAAAVALLLVGGVVVEAL
                     NHGGAETPARATVASAFQPAKGNATEQAKGTQVVANTQVLPPIDAGHVVQVPLGLGAG
                     GGPAVAAATPEPTVPALAYAGTLPELPGTARSWLLSAPPSASTFVQNELARIGVTGTV
                     KEETQNGATVVLGLDETGFPVIRWRPADAFFSFDRGPNMPTPPPARADTDPKQTATDW
                     LEEIGFDLNTIHYRANLRADSTAQLRVVEFRPLDLPPGAIDPGLGVIVGVRPDGSISY
                     AYGFWLSLVESAEVPLRPVTQAWQAAQRGEGLIPSFGPPPRYKAIAVKSVQLAYLLTR
                     ADESSYVLEPVAAFTGEVEGTSEAGQVTIYVAAVKPPEPATPQP"
BASE COUNT          357 a          906 c          917 g          349 t
ORIGIN      
        1 atgctgccga ttgcctggag actcaagccg gcggcttgcg cgcggcgaat gaagcgcagg
       61 cgcgtgagat cggcctccgt gtagcagcgg tagccgttgg tggcccgtcg cggagccggg
      121 agcaagccga acgtttcata gtagcggatc gttttggccg tgaccccggc ccgagtggca
      181 agcacgccaa tccgcatagc gccgtccttc ctcgaatggt catcaccgca gccatcgcac
      241 aacacggtac gccggtcagt caagcagtcg aagtctggag cctggggctg gtttgcgcgt
      301 gaaccggatc agcacgctgc cgcagtgggt gatgggcggc atgttccccg tgagccgccc
      361 gccgcgacgc tgtgcggtcg ttcactgtgg cgagaaccgc agcgcgcggc ggacggccca
      421 ggcctgggct tcttcgtgga gggcggtgag ggcgtcgtgg ggtgcgagcc agcggagggt
      481 gtggtccggc tcggatgtgc cgtcgacgcg ggcgccaagc tcgaccagga agaaggtctc
      541 cagcttgttg tagcagatgc cggtcgagcg ctcgatcacg tactggccgg cggcgccgag
      601 ctcgcgcaga gcggcgagct ggtagccggt ctcctcgcgc agctcgcggc gcagggccgc
      661 ctcggcgctc tccccggttt cccggccgcc gcccggcagg aagcgcccgg ccggcgtctc
      721 gaccagcagc aagcgcccgg cgacgagcgc caccgcatag acgcctggac gatctcggca
      781 gatcccatca gccggcggct gcccgaactg ggggtgaggc ggcaaggccg atcagcgcag
      841 cacgtggcgg ctggtcatca cgcccagcgc gagcatgact agccccgata cggccgcggc
      901 cagcccgcga gcgcgcgcct gcagcagcaa cgcggcgccg tcgagcgcga tcgccgccgc
      961 tgctagcccc gagagggcga ccatcaacgg ccgcagtggt gtggccagcg aactgcggcg
     1021 caccgccatg gcggtcagaa agagtccaaa gagagtggct acgctgacca gcggcggcag
     1081 ccagagcggt cgctcgcgtt gcgaccaggt taagcttcgc ggcatcgctg ctcctttcat
     1141 ggttgcgggg tcgcgggctc cggcggcttg accgccgcga cgtagatcgt cacctgcccg
     1201 gcctcggacg ttccctcgac ctcgccggtg aaggcggcca ccggctccag gacatagctg
     1261 ctctcgtcgg cgcgcgtcag caggtaggcc aactgcaccg acttcaccgc gatcgctttg
     1321 tagcgcggcg gcgggccgaa gctcgggatc aggccctcgc cgcgctgcgc cgcctgccag
     1381 gcctgcgtca ccggccgcag cggcacctcc gccgactcca ccagcgagag ccagaacccg
     1441 taggcgtagc tgatcgagcc gtccggccgc acgccgacga tcactcccag accgggatcg
     1501 atcgcacccg gcggcagatc gagcgggcgg aactcgacca cgcgcaactg cgccgtcgag
     1561 tcggcgcgca ggttggcgcg gtagtggatg gtgttgagat cgaagccgat ctcctccagc
     1621 cagtcggtcg ctgtctgttt ggggtcggtg tcggcgcggg ccggcggcgg ggtcggcatg
     1681 ttcgggccgc gatcgaacga gaagaaggcg tcagcggggc gccagcggat caccgggaag
     1741 ccggtctcgt cgagcccgag caccactgtc gcgccgttct gcgtctcctc cttgaccgtg
     1801 ccggtgacac cgatgcgcgc cagctcgttc tgcacgaagg tgctggccga gggcggcgcg
     1861 gagaggagcc aggagcgcgc cgtgcccggc agctccggca gcgtgccggc gtaggcgagc
     1921 gccgggacgg ttggttccgg cgtggccgcc gccaccgccg gcccgccccc ggcgcccagc
     1981 cccagcggca cctgcaccac gtgcccggcg tcgatcggcg gcagcacctg ggtgttggcc
     2041 accacctgcg tgcccttggc ctgctcggtg gcgttgccct tggccggctg gaaggcgctg
     2101 gcgacggtgg cgcgcgccgg cgtctccgcg ccgccgtggt tgagcgcctc gaccaccacg
     2161 ccgccgacca agagcagcgc caccgccgcc gcgatcactg ccacgcgcca atcctgatac
     2221 cagtgccgcg gaaggggggt gatgaccggc tcgcccagca gttcctggcg caggcgaaag
     2281 gcggggtcgg gcagctcggg cggcagctcg cgctccaggc gcccggccag cgccgccagc
     2341 tcgcgcagct cggcgctcag ctccggctca tcctcggccg attgcccgcc ggcacgccgg
     2401 cgcgcgagcg cccggtcgag ctgctcggcc tgctcgcgct cagacaccac gcccctcttc
     2461 cagctccggg tcggcccgca gcgcgcgcag cgcgcgatgg accaactgct tggcggcgcc
     2521 ctcgctgcg
//
LOCUS       DASUBI010000160         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1031108, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000160 DASUBI010000000
VERSION     DASUBI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031108"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFU72_02995"
     CDS             <1..752
                     /locus_tag="VFU72_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422484.1"
                     /translation="LGLAAGAGEFLKWVGIEQRTPLATTREAMTAVRALLAGEPVAGW
                     LPEAYLRFPARPLPIYLGAFGPGMLRLAGEVADGVLPLLFPPEHIETVLPLVEAGAAR
                     AGRSLEEIDVAACVWCSVAEDRAAAEDALKDKIAYYGHALGPLIWDRLGLTQADFRPI
                     EHALMTERDPARARALVDERMLRIGVVGTPADLIARLEGLVVRGVRHLSFGPPLGPDV
                     AAAIVAIGREVIPHFRSGEQEETAGWGYRRY"
     gene            complement(869..1774)
                     /locus_tag="VFU72_03000"
     CDS             complement(869..1774)
                     /locus_tag="VFU72_03000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422485.1"
                     /translation="MAQPAPLLGINIDPAIERQEESFERAAIADRAGLDLATSQDHPY
                     IPHFFDTWTLLAMLAARTERLHLGSNVSPLPLRPPVMLAKAVASLDVLSGGRVELGIG
                     AGGFEAAIAAFGGRLPPRTEIVDDFAEAIELIRGLWRAERSFRFSGEHYQLKGTRFGP
                     APAHPLRIWVGATKPRMLRLTGRLADGVLVSNGYVPEAELAEVNRLIDQGAARAGRAP
                     EAIRRGYNLMGAIDLPGHPNQPEDLRPGTPLLPADGWVTHLVELYRVRRMDTFIFWPL
                     GERQLEQVEVFATEIAPQVRLALGA"
     gene            2073..2435
                     /locus_tag="VFU72_03005"
     CDS             2073..2435
                     /locus_tag="VFU72_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422486.1"
                     /translation="MSDAFRRHLISLREQVSVSVELAAQASREYHEVWSQRKGLATRL
                     GSLEPEAESLESLRRREMLYLGEAAALQWILGRLDQLVAELESGQCADDHIVRESSRA
                     PGTEPADDSRPDAAVVDD"
     gene            2535..4157
                     /locus_tag="VFU72_03010"
     CDS             2535..4157
                     /locus_tag="VFU72_03010"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU5422487.1"
                     /translation="MSSDLQREVARRRTFAIISHPDAGKTTLTEKLLLYGGAVQLAGS
                     VTARKQQRQTASDWMALERERGISISSTVLQFAYDGYQINLLDTPGHQDFSEDTYRTL
                     MAADSAVMVLDAARGIEPQTLKLFEVCRLRRIPIFTFINKLDRPARDPLDLLDEIERV
                     LGLAVYPMNWPIGDGPQFQGIYDRAAQAVLRYDRTEHGARRAPVQMAGLDDPDLDGLL
                     GAEPAVKLREAVELLEVAGYREDHERVLAGELTPVFFGSAVTNFGVQPFLDAFVALAP
                     PPRLRQAAERPVCPEDEAFSGFVFKVQANMDPRHRDRVAYLRVCSGRFEKDMQVRHLR
                     LGRDVRLSRPLRLFGQEREVVEEGYAGDVIGLINPGLFVVGDTVASGSESAGPFEALP
                     RFQPEHFALLRNARADRYKQFQRGLSQIEEEGGIQLLYPLDSGSREPILAAVGALQFD
                     VVRYRLEHEYGVETILEPLGYALARWVEGDPEEIRQLAGRRGRQRAEDVAGNPVVLFR
                     TSWDLDFAVEEHPAVRFTTLGGAPAALAAAGD"
     regulatory      4280..4364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4413..>5712
                     /locus_tag="VFU72_03015"
     CDS             4413..>5712
                     /locus_tag="VFU72_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5422488.1"
                     /translation="MLFTPPGPGALPPPRWAEIEVPSRRVAAGGANLRGAAIDRPRDS
                     WWQSRWRFLTIGVLLLCVPLLGILPADAAPPDNGPHADAGLLHQAAGNPNQLFRVIIQ
                     GKGEHGRAAADNAAAAAHVTTLNDVADVGVVVQISGKALAALAKAPGVKYISLDSPTR
                     PAACSSSGPVNSCSLTTLYPQATSATSLWPGGLTGKGVGVAVVDTGVSSKPDFDNRIV
                     VNQKFNAATTNQNDGYGHGTHVAGIIAGNSWSSSNSSVRGLYIGVAPQANIVNVKVAD
                     DTGMAYTSDVVNGIDWAITNRTAYNIRVLNLSMVQSTPESYHTSILDAEVEKAWFNGI
                     LVVVAAGNAGPGTALYPPMNDPFVVTVGAADVNNTADQRDDFLAPWSTYGATQDGVVK
                     PDLVAPGRVMYSVLASKSVVLAQLFPSRISPDGNYIWLSGT"
BASE COUNT          870 a         2044 c         1978 g          820 t
ORIGIN      
        1 ggctcgggct ggcggccggc gccggcgagt tcctcaagtg ggtgggcatc gaacagcgca
       61 caccgctggc gactacgcgc gaggccatga cagctgtgcg cgcgctgcta gcgggcgagc
      121 cggtcgccgg ctggctgccc gaggcctacc tgcgcttccc cgcgcgcccg ctgccgatct
      181 acctcggcgc cttcggcccg gggatgctgc ggctggccgg cgaagtggcc gacggcgtcc
      241 tgccgctcct cttcccgccc gagcacatcg agacggtcct gccgctggtc gaggcgggag
      301 cggcccgcgc cggtcggtcg ctggaggaga tcgacgtggc ggcctgcgtc tggtgctcgg
      361 tggccgagga ccgcgctgcc gccgaggacg cgctcaagga caagatcgcc tactacggcc
      421 acgccctcgg cccgctgatc tgggaccggc tcgggctgac ccaggccgat ttccgcccca
      481 tcgagcacgc gctgatgacc gagcgcgacc cggccagggc ccgggcgctg gtcgacgagc
      541 gcatgctgcg catcggggtc gtcggcacgc cggccgatct catcgcgcgc ctggaggggc
      601 tggtggtgcg cggcgtccgc cacctcagct tcggcccgcc gctcggcccc gacgtggccg
      661 ccgcgatcgt cgccatcggc cgcgaggtca tcccccactt tcgatccggc gaacaagagg
      721 agaccgccgg ttggggttac cggcggtact gaggaagaga agggggaatg gtggggttgg
      781 tcacggtttg tagtctgcgg ccccaccctg aaaccggggt gaaaacggcc tgaaaggttt
      841 ctgagcaaat cgtgccgggg ccaaaggatc aggcgccgag ggccaggcgc acctgtggcg
      901 cgatctcggt ggcgaagacc tcgacctgct ccagttggcg ctcgcccagc ggccagaaga
      961 tgaaggtgtc catgcgccgc acccggtaga gctcgaccag gtgcgtcacc cagccatcgg
     1021 ccggcagcag cggcgtgccc ggccgcaggt cctccggctg gttcgggtgg ccggggaggt
     1081 cgatggcgcc catcagattg tagccgcgcc ggatggcctc cggcgcgcgc ccggcgcgcg
     1141 ccgcgccctg gtcgatcagc cggttgacct cggccagctc cgcctccggc acgtagccgt
     1201 tcgagaccag cacgccgtcg gccagccggc cggtcaggcg cagcatgcgc ggcttggtgg
     1261 cgccgaccca gatgcgcagc gggtgcgccg gcgcggggcc gaagcgggtg cccttgagct
     1321 ggtagtgctc accgctgaag cggaagctgc gctcggctcg ccagagcccg cgaatcagct
     1381 cgatagcctc ggcgaagtcg tcgacgatct cggtgcgcgg cggcagccgc ccgccgaagg
     1441 cggcgatggc ggcctcgaag ccgcccgcgc cgatgcccag ctcgacgcgg ccgccggaga
     1501 gaacatcgag cgaggcgacc gccttggcca gcatcaccgg cgggcgcagc ggcagcggcg
     1561 agacgttgga gccgaggtgc agccgctcgg tgcgcgccgc cagcatggcc agcagcgtcc
     1621 aggtgtcgaa gaagtggggg atgtaggggt ggtcctggct ggtagccagg tcgagcccgg
     1681 cccggtcggc gatcgcggcg cgctcgaaac tctcctcctg ccgttcgatc gccgggtcga
     1741 tgttgattcc cagcagcggc gccggctgtg ccatctggtt cgtccttacc tcctaccgcc
     1801 caggccattc ctgggagcgg cgaccggttg tctgctggga tcatacgcga gcatgctgcc
     1861 aaacgcctgc cggagcccat ccaagaccgg gcacgaagca ctaccgccgc caagtacgac
     1921 tagcccgaaa gtaccggcgg cgcctgggcg gtattctcca gggaggagcg ggaagatagt
     1981 gggagattgc ccgctacgga ggaccacgat atgctcgata gtgcgcttga gagctgctag
     2041 ggggctcagc ccgacaatag aggaggggcg cgatgagcga cgcgtttcgc cggcatctga
     2101 tcagcctgcg cgagcaggtc tcggtctcgg tcgagctcgc cgcgcaggcc tcgcgcgagt
     2161 accacgaggt ctggagccag cgcaaaggcc tcgccacgcg cctgggctcg ctggagccgg
     2221 aggccgagag cctggagagc ctgcgccgcc gggagatgct ctatctcgga gaggcggccg
     2281 cgttgcagtg gatcctgggc cggctcgatc aactggtggc cgagctggag tcggggcaat
     2341 gcgcggacga ccatatcgtg cgggagtcta gccgagcgcc gggtacggaa ccggcggacg
     2401 actcgcgccc cgacgcggca gtcgtcgacg actagcgcgc cgggcggctc ctcggaccgg
     2461 acgtgcctgg tacaattggt tgtttgggcg cgcggtgggc gtgccgcggc gcgatccaat
     2521 ccgaggagca gcaggtgtca tccgatcttc agcgtgaggt cgcccggcgg cgcaccttcg
     2581 ccatcatctc gcaccccgac gccggcaaga ccacgctgac cgagaagctg ctgctctacg
     2641 gcggcgcggt ccagcttgcc ggctcggtca ccgcgcgcaa gcagcagcgc cagacggcct
     2701 ccgactggat ggcgctggag cgcgagcgcg gcatctcgat ctcctcgacc gtgctccagt
     2761 tcgcctacga cggctaccag atcaacctgc tcgacacccc gggccaccag gacttctcgg
     2821 aggacaccta ccgcaccctg atggcggccg acagcgcggt gatggtgctc gacgccgccc
     2881 gcggcatcga gccgcagacg ctgaagctct tcgaggtctg ccgcctgcgg cgtatcccga
     2941 tcttcacctt catcaacaag ctcgaccgcc cggcgcgcga cccgctcgac ctgctcgacg
     3001 agatcgagcg cgtcctcggg ctggcggtct accccatgaa ctggccgatc ggcgacggcc
     3061 cgcagttcca gggcatctac gaccgcgccg cccaggcagt gctgcgctac gaccgcaccg
     3121 agcacggcgc gcgccgcgcc ccggtgcaga tggccgggct ggacgacccc gacctggacg
     3181 gcctgctcgg cgccgagccg gcggtcaagc tgcgcgaggc ggtcgagctc ttggaggtag
     3241 cgggctatcg cgaggaccac gagcgcgtgc tggccggcga gctgacgccg gtcttcttcg
     3301 gcagcgccgt gaccaacttc ggcgtgcaac ccttcctcga tgccttcgtc gcgctggcgc
     3361 cgccaccgcg cctgcgccag gcggccgagc gcccggtctg ccccgaggac gaagccttct
     3421 ccggcttcgt cttcaaggtg caggccaaca tggacccacg ccaccgcgac cgcgtggcct
     3481 acctgcgcgt ctgctccggc cgcttcgaga aggacatgca ggtgcgccac ctgcgcctgg
     3541 ggcgcgacgt ccggctcagc cgcccgctgc ggctcttcgg ccaggagcgc gaggtggtcg
     3601 aggagggcta cgccggcgac gtgatcggcc tgatcaaccc cggcctcttc gtggtcggcg
     3661 acaccgtcgc gagcgggagc gagagcgccg gccccttcga ggcgctgccg cgcttccagc
     3721 cggagcattt cgcgctcttg cgcaacgccc gcgccgaccg ctacaagcag ttccagcgtg
     3781 gactgagcca gatcgaggag gagggcggca tccagctcct ctacccgctc gactcgggca
     3841 gtcgcgagcc gatcttggcc gcggtcggcg cgctgcagtt cgacgtggtg cgctaccggc
     3901 tggagcacga gtacggcgtc gagacaatcc tggagccgct cggctacgcg ctggcgcgct
     3961 gggtggaggg cgacccggag gagattcgac agctcgctgg gcggcgtggc cgccagcgcg
     4021 ccgaggatgt cgcgggcaac cccgtggttc tcttccgcac cagttgggat ctcgacttcg
     4081 ccgtcgagga gcaccccgcc gtgcgcttca ccaccctcgg cggcgccccc gccgccctgg
     4141 ccgccgccgg cgactagcgc ccggtactca actcccaggc caatcttccc ggcctgatct
     4201 cccgcccgcg cagagccgat cggctctacg ttgccgcgcg gcgcccgcct atcctgaagc
     4261 caatagtgat ctcgggagat cacgacaaag gcacacccgg cgaaagccgg ggccgcaaag
     4321 cgacaggtct acggccaccc ggctacgacg gctgcgctac cgaaggtctc cctctttgtt
     4381 tagccgggat ggcgagcaag agggagaaag tcatgctctt cacacccccg ggcccaggcg
     4441 ccctgccgcc cccacgctgg gccgagatcg aggttccgag ccgtcgcgtc gccgcgggcg
     4501 gagcgaacct gcgcggcgcg gcgattgacc ggccgcgaga tagctggtgg cagagccgct
     4561 ggcgcttcct gaccatcggc gtcttgctgc tctgtgtgcc gctgctgggc attctgcccg
     4621 ccgacgcggc cccgcccgac aatgggccgc acgcggacgc cgggctgctg caccaggcgg
     4681 ccggcaaccc caaccagctc ttccgcgtca tcattcaggg caagggcgag cacggccgcg
     4741 ccgccgcgga caacgcggcc gccgccgcgc atgtcacaac gctcaatgac gtggcggacg
     4801 tcggcgtggt cgtgcagatc tcgggcaagg cgctggccgc gctggccaaa gcgccgggcg
     4861 tcaagtacat cagcctcgac tctccgacgc ggcctgctgc ctgctccagc tcggggccgg
     4921 tcaactcgtg cagcctgacg acgctctatc cccaggccac cagcgccacc agtctctggc
     4981 cgggcgggct gaccggaaag ggcgtcggcg tggcggtggt cgataccggc gtctccagca
     5041 agcccgactt cgacaaccgc atcgtggtca accagaagtt caacgcggcc accaccaacc
     5101 agaacgatgg ctacgggcac ggcacccacg tcgccgggat catcgccggc aactcctgga
     5161 gcagctcgaa tagctccgta cgcggcctct acatcggcgt ggcgccgcag gccaacatcg
     5221 tcaatgtcaa ggtcgccgac gacaccggca tggcctacac ctccgatgtc gtcaacggga
     5281 tcgactgggc catcaccaac cgcacggcct acaatatccg cgtcttgaat ctctccatgg
     5341 tacagagcac gcccgagagc taccacacct cgatcctcga cgccgaggtg gagaaggcct
     5401 ggttcaacgg catcctggtg gtggtcgccg ccggcaacgc cggccccggc acggcgctct
     5461 acccgccgat gaacgatccc ttcgtggtca ccgtcggcgc ggcggacgtg aacaacaccg
     5521 ccgaccagcg cgacgacttc ctggcgccct ggtcgaccta tggcgcgacc caggacggcg
     5581 tcgtcaagcc cgacctggtg gcgcccggcc gcgtgatgta ctcggtgctg gccagcaaga
     5641 gcgtcgtcct ggcgcagctc ttccccagcc ggatcagccc cgacggcaac tacatctggc
     5701 tcagcggcac gt
//
LOCUS       DASUBI010000161        13094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_663385, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000161 DASUBI010000000
VERSION     DASUBI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663385"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFU72_03020"
     CDS             complement(<1..476)
                     /locus_tag="VFU72_03020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5422489.1"
                     /translation="MARSRPKLVCIADIHGHYDELIGLYNQLLAGGFDPERDTLVQLG
                     DIVDGGPDTQAVVTWCMAMAKRYPHWVFLKGNHEDLLLDALLFHCRLYGSYHLWWEQG
                     GQATAQSYRPHDASAYERALMHPCDYIPLAHLVWLAARPLYYATDRYLFVHAGLRPG"
     gene            complement(639..800)
                     /locus_tag="VFU72_03025"
     CDS             complement(639..800)
                     /locus_tag="VFU72_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422490.1"
                     /translation="MSKGKKRTEAEKSAQRARTAKNKINAIQRALQHAGGTHIARLQE
                     RLAFWQGKR"
     gene            complement(826..1218)
                     /locus_tag="VFU72_03030"
     CDS             complement(826..1218)
                     /locus_tag="VFU72_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422491.1"
                     /translation="MTHLNHSWPIGKRYDLTLRTLPAFLDHFNPRTPLYDELLPLRYR
                     ARQAQDWRLADAIRDYAELAYGYTIIDSPIAYPEDGHDFQHFRPVRLIAWYKKWRPYI
                     VSHAYEDTWLDRLATRGLMPYAPGWAAI"
     gene            complement(1399..1545)
                     /locus_tag="VFU72_03035"
     CDS             complement(1399..1545)
                     /locus_tag="VFU72_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422492.1"
                     /translation="MEELTFSLVHDLGCRPQGSSGGGIGFAGLLTIVFITLKLTGYIH
                     WPWL"
     gene            1586..1891
                     /locus_tag="VFU72_03040"
     CDS             1586..1891
                     /locus_tag="VFU72_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422493.1"
                     /translation="MTHGRSHSREFKLDVVRQIARGEKRPAQICREYGLAEGLLLRWR
                     KEYDARGEAAFGPSGDGENRELAQRVAELERLCGQLALENAALQRGLRLSRSRSSTR"
     gene            1888..2745
                     /locus_tag="VFU72_03045"
     CDS             1888..2745
                     /locus_tag="VFU72_03045"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5422494.1"
                     /translation="MIAELRASFPDLSVRRLCALLGTGRTWYYTRPTAEARAARDTAL
                     RDAIERVVLDFPGYGYRRVAKALQRAGWEINHKRVLRVMREEALLCQLKRRFVVTTDA
                     AHDCRTYPNLLAGLSIARLDQVWVADITYIRLPTAFAYLACVLDACSRRCIGWQLSRQ
                     IDTGLTLAALDHALAARRPVPGLIHHSDRGVQYASADYVARLEAAGCRVSMSAKGNPY
                     DNARAESFFKTLKREEVSLQQYQTFAEAERNLTRFIEDVYNQKRLHSSLGYRPPAEFE
                     ALAAATTGS"
     gene            complement(2816..3625)
                     /locus_tag="VFU72_03050"
     CDS             complement(2816..3625)
                     /locus_tag="VFU72_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422495.1"
                     /translation="MHLLPRKGPGRPRTKAPEIRVTVYSTDYHIPFHDVAAYRAFCHF
                     LAYVRPDYHIVLGDFIDATMLSTFAKDPAEANRTEEELLTANAVLDELQAASPNTITK
                     YVMGNHSKRLTKRLWDNPHLIPYLGGKTPLQLLANCLSLDERGIEYFDYPSGYDHFGI
                     YCTHGEATGMHSSKNELETYCASGISGHTHKNRYWEKRGRNGVTQWWTIGGLCSRNVS
                     YRPNNDWVQGIAISFQVVGTDVFTMLPITITKGQLIYDRHLFTQDGVFTCQ"
     gene            complement(3837..4118)
                     /locus_tag="VFU72_03055"
     CDS             complement(3837..4118)
                     /locus_tag="VFU72_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422496.1"
                     /translation="MTIDDEAPEVFHFDVESIGAGTWKVMGIVSNNLTTDAARFETAL
                     QRYLEDPMDITRLHDIHNAIDLVLEQRNMIIPLLESFSRYLDQHLKSPE"
     gene            complement(4294..4479)
                     /locus_tag="VFU72_03060"
     CDS             complement(4294..4479)
                     /locus_tag="VFU72_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422497.1"
                     /translation="MNEQVWQTLQDEGEVTVEVTLAEFDRMIQALNAAQLAFQFSGQS
                     TDTNTLICTVTLKDDEE"
     gene            complement(4566..5327)
                     /locus_tag="VFU72_03065"
     CDS             complement(4566..5327)
                     /locus_tag="VFU72_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422498.1"
                     /translation="MGPIRFAWTVNRALITGLVVGIVLGGFVMTKATAAGIPLPVYQT
                     PTARVVVQQIPMTVVVTQPAPVVASNQQQAPSANTQSQGAAPAGPTSPPVPTPTEGPT
                     PTPTPIPTPTATPTPKPTPTPHPLTTAGTVLKNGETWYADGATLQVTVTMSVTFFPLG
                     TFINFTYTNTSDQPQEFDFTRDNIHVTYAPGSVEAAGSSDTTHWYLAPGQSRSLKVSA
                     NLPSGDTHITVTFTNFGHIESAKWGMIWANNQLVP"
     gene            5722..6261
                     /locus_tag="VFU72_03070"
     CDS             5722..6261
                     /locus_tag="VFU72_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422499.1"
                     /translation="MEGSRTYGRGLAQFLVAAGAEVYEINPRWTAAGRWRARRPDKND
                     RRDAQAIALLVWREAAELPRVVADDETAVLDLLVTEREDVVAEMTRLKNQLHQLLLQL
                     DPEYGARLPSLRTQAGLHALEGYTVTDPTPVQQQRAGTVRRLAARLRLVTEQAKALAT
                     QIWTLAAPPSPFLAPQAGH"
     gene            complement(6658..7428)
                     /locus_tag="VFU72_03075"
     CDS             complement(6658..7428)
                     /locus_tag="VFU72_03075"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5422500.1"
                     /translation="MQLSTALPHLMGFQIELVQITADAITVHAATCGDAACCPLCGER
                     SERVQSRYQRTVVDRPVGGRWVFLVLQVRRFSCANVACPRRIFAERFPDLAAPYARRT
                     IQQQADLRQLGLALGGRAGARLAARLGLASSHDTILRLAAGAPTPDTGAPRVVGIDDF
                     AFRRGLTYGTLIVDLERRRPLDVLPGRAADTVGQWLAARPSSEVAVRDRYDADAVAAA
                     QGAPQACQVVDRCQLLRNLGDAVEKFLFTKTAVLRQVH"
     gene            complement(7624..8433)
                     /gene="heR"
                     /locus_tag="VFU72_03080"
     CDS             complement(7624..8433)
                     /gene="heR"
                     /locus_tag="VFU72_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEU5422501.1"
                     /translation="MRAVSAEVERVAPPGRTSAGARPFFTFNLVMAGLHGAQALAMLL
                     LSQDFRLPIIVSYLAFNEQTQRLDPAIQQIGRVPLAWLMVGFLLLSALAHASIATLYR
                     GRYERELALGLNRARWIEYSLSASVMMVGIALLVGVYDLGSLVLIFGLVAVMNLLGLV
                     MELDNQGVAPRRWSSFWVGCLAGLVPWFVVGLYFLASSRYGGGEIPAFVYGIYVSIFL
                     CFNCFAVNMYLQYKQIGPWRDYLFGERAYMILSLVAKSALAWQVFAGTLRP"
     gene            complement(9029..9393)
                     /locus_tag="VFU72_03085"
                     /pseudo
     CDS             complement(9029..9393)
                     /locus_tag="VFU72_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(10561..11670)
                     /locus_tag="VFU72_03090"
                     /pseudo
     CDS             complement(10561..11670)
                     /locus_tag="VFU72_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            11841..12674
                     /locus_tag="VFU72_03095"
     CDS             11841..12674
                     /locus_tag="VFU72_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEU5422502.1"
                     /translation="MRRLIQGAVCGNPGLPLPGDLNPRPRGLQATGPAQAEFVVCDQA
                     IDSTYVATQEGWRYLAVRLDAHARRVIGWALADHLRTELALDALGMALEWRRPGAGLV
                     PHTARGGQDTAGAYRAALAARSVTVSMSRSGACRDNALSERCVATRKAEIADAQVWLS
                     RAAARTAIFAWIAVWYNRQRRHSALADQSPACCEEEVVLWSCPAASRYAVHEIGATSV
                     CNPGSPVPATSAMIAGTGTALRASSRTCHSRTFGRTQLPPPWRRACSVDGRGGGGWPR
                     G"
BASE COUNT         2211 a         4255 c         4413 g         2215 t
ORIGIN      
        1 cctgggcgta acccggcatg gacaaagaga tagcggtcgg tcgcgtaata gagcggccgg
       61 gcggcgagcc agaccaggtg ggcgagtggg atgtagtcac aggggtgcat gagcgcgcgc
      121 tcgtaggcgc tggcgtcgtg agggcggtac gactgggcgg tggcctggcc accttgctcc
      181 caccacaggt gataagagcc atagagccgg cagtgaaaaa ggagcgcatc aagcagcagg
      241 tcttcatggt tgcctttcag aaagacccaa tgcggatagc gtttggccat cgccatacac
      301 caggtcacga ccgcttgggt gtccgggccg ccatcaacga tgtcacccag ttgcacgagg
      361 gtatcgcgct ctgggtcgaa gccgccggcc aggagctggt tatatagccc gatcagttcg
      421 tcatagtgcc cgtgaatgtc ggcgatgcag acgagcttag gccgggaccg ggccatagag
      481 cagtgtcctt tcttatacgg tgacgaggag tgtgcggact acaagaggga ttatcgtccg
      541 gccagggctg cgcttgtctg aatttccgga tcgaagaatc tgacaaattt ggcgtcatgg
      601 tcgtctattt cgccgccgca gatgctcggc aaggctggct agcgcttccc ctgccagaag
      661 gccaaccgct cttgcaggcg ggcgatatgt gtgccgccgg cgtgctgtaa ggcgcgctgg
      721 atagcgttga ttttattctt ggccgtgcga gcgcgttggg cggatttttc ggcttcggtg
      781 cgtttctttc ctttagacat agatagttcc tccaaaataa tgcggtcaga ttgccgccca
      841 tccaggtgca tacggcatga gaccacgcgt ggctagacga tccagccagg tatcctcata
      901 ggcatgactg acgatgtaag gacgccactt cttataccag gcgatcagcc gcactggcct
      961 aaaatgctgg aagtcgtggc cgtcctcggg atacgcgatg gggctatcaa tgatggtgta
     1021 tccataggca agctcagcat agtcccggat cgcgtcggcc agtcgccaat cttgcgcttg
     1081 tctggcccgg tagcggagcg gtaacagctc gtcataaagc ggtgtacggg gattgaagtg
     1141 gtcgaggaac gccggcaagg tgcgaagcgt gaggtcatag cgcttaccaa tgggccacga
     1201 gtggttgaga tgcgtcataa atattgatgg acggcgggag gtttccctcc attccggtca
     1261 ggcacgtctg aatgctgccg gtcgatcacg ccgcccgtaa ggtctctact tcaacacgtc
     1321 acagatcatg acaacggcga gcacaatgcc gatgactaca agcgcgatca ccagactgat
     1381 ccaaagcggg gataagacct aaagccaggg ccaatgaata tagcccgtga gtttgagggt
     1441 gataaagacg atggtcagca ggccagcaaa gccgatgccg ccgcctgatg aaccctgtgg
     1501 acggcacccc aaatcgtgaa ccaggctgaa agtcaactcc tccattcggg cggtagactc
     1561 ccgctcagga agaaggagga gcacgatgac ccatggacgg agccatagcc gcgagttcaa
     1621 gctcgatgtc gtccggcaga tcgcgcgcgg ggagaaacgc ccggcgcaga tatgccgcga
     1681 gtacggcctc gccgagggcc tgctgctacg ctggcggaag gagtacgacg cgcgtggcga
     1741 ggccgccttt ggcccctccg gggacggcga gaatcgggag ttggcgcagc gggtcgcgga
     1801 gttggaacgg ctgtgtggcc aactcgcgct ggagaacgcg gcgctacaaa ggggcttgcg
     1861 cctctcgcgc tcgcggagca gcacgcgatg atcgccgaac tgcgcgcgtc attccccgac
     1921 ctgtcggtgc gtcgcctctg tgcgctgctc ggcaccgggc ggacgtggta ctacacgcgc
     1981 ccgacggccg aggcgcgggc ggcccgcgac acggcgctgc gcgacgccat cgagcgcgtc
     2041 gtgctcgact tccccggcta tggctatcgg cgcgtggcaa aagccttgca gcgtgcgggg
     2101 tgggaaatca accacaagcg ggtgctccgg gtcatgcgcg aggaagccct gctgtgccag
     2161 ctcaagcggc gtttcgtcgt cacgactgac gcggcccacg actgccgcac ctacccaaac
     2221 ctgctcgccg ggctgtcgat cgcccggctc gaccaggtct gggtcgcgga catcacgtac
     2281 atccgcctgc cgacggcctt cgcctacctc gcctgcgtgc tggatgcctg ctcccgacgc
     2341 tgcatcggct ggcagctttc ccgccagatc gacacgggcc tcacgctcgc cgcgctcgac
     2401 cacgccctgg ccgcccggcg gccggtgccg gggctgatcc accactccga tcgcggggtc
     2461 cagtacgcca gcgccgacta cgtcgcccgg ctggaggcgg ccggctgccg ggtcagcatg
     2521 tccgccaagg gcaatcccta cgacaacgcc cgcgcggaga gtttcttcaa gacgttgaaa
     2581 cgcgaggagg tgtccctgca gcagtaccag accttcgccg aggccgagcg caacctcacc
     2641 cgcttcatcg aggacgtcta taatcagaag cgcctgcact ctagccttgg ctaccggccc
     2701 cccgccgagt tcgaggcatt ggcggcggcg accaccggga gttgacttta cccgtggttc
     2761 gcaattaggg gttcactcca ctgtgatgat ttagacatag atactccgtt accctttact
     2821 ggcaggtgaa cacgccatcc tgggtaaaga gatggcggtc gtagatcagc tggcctttgg
     2881 taatcgtgat gggcagcatg gtaaagacgt ctgtgccaac cacttggaag gaaatggcga
     2941 ttccctgcac ccagtcgttg ttgggccggt aactcacatt gcggctacac aggccgccaa
     3001 ttgtccacca ctgcgtgacg ccattacggc cgcgcttctc ccagtacctg ttcttatgcg
     3061 tatggccgct tatccctgac gcgcaatagg tctccaactc gttcttgctg gaatgcatcc
     3121 ccgttgcttc gccgtgggtg cagtaaatac caaagtggtc atagccagac gggtaatcaa
     3181 agtattcaat gccgcgttcg tcgagggata ggcagttcgc cagcagttgc agcggtgtct
     3241 tgccgcccag gtagggaatg aggtgcgggt tgtcccacag gcgcttggtc agtcgcttac
     3301 tgtggttgcc catgacgtac ttggtaatcg tattgggtga ggctgcttgc agctcatcga
     3361 gcacggcgtt ggcggtgagc agttcttctt ccgtgcggtt cgcctcggcc ggatctttgg
     3421 caaaggtgga gagcatcgtg gcgtcaataa aatcgcccag tacgatgtgg tagtcgggtc
     3481 gaacgtaggc gaggaagtgg caaaaggcgc ggtaagcggc cacgtcatgg aaaggaatgt
     3541 ggtaatccgt gctatagacg gtgacgcgaa tttcgggtgc tttcgtgcgg ggtcggccgg
     3601 ggcctttgcg gggcagcaag tgcatagata ttgggtagta tttgtcccct tataacagcc
     3661 aagcgaggtt attgtcaagc acgcggattt gaaggaacgg tccgaaaccg aagaggtgag
     3721 tacgcttgtc tcttctgttc tggagcgatt aagtaatccc atgtacgggc attatcgtga
     3781 tccagccagg tagggcccta tggttattcc gataaagacg cgcttagtgg tttagatcac
     3841 tcgggcgact taagatgttg atcgaggtag cgagagaaag attctaagag gggtatgatc
     3901 atattgcgtt gctccagaac caagtcaatc gcattgtgta tatcatgaag gcgggtgata
     3961 tccatcgggt cttccaaata gcgttgcagg gctgtttcga atctagccgc atcggtagtc
     4021 aggttgtttg agacgatgcc cataactttc caggttcccg caccaattga ttcaacatcg
     4081 aagtgaaata cttccggcgc ttcatcgtcg attgtcatca tcttccccaa tctgtagcga
     4141 atagaccagg cagctcagga gtcagtctct tcattgtcca ccgacgatgc ctcttggact
     4201 ttcaagaagc cgtccgcgtt gaaatgggcc tgggtaagtt ctaaggcgca gcccaggcta
     4261 cgaacggagg tggcggggcg ggtaagacgt cgcctactcc tcgtcatcct tcaaagtaac
     4321 ggtgcagatc agtgtgttgg tatcggtgga ctggccgctg aattgaaagg cgagctgcgc
     4381 cgcgttgagc gcctggatca tccggtcaaa ctccgccagc gtcacctcta ccgtgacttc
     4441 gccctcatcc tgcaaggtct gccagacctg ctcgttcacg gtggttgctc cggactgcca
     4501 tcagcgcgtg gctcggtccg attatatccc ggcgtcagtg ccgtaagcac agcttctcgc
     4561 ctctcctatg ggacaagctg attatttgcc caaatcatcc cccacttggc gctttcaatg
     4621 tgcccgaagt tcgtgaaggt cacggtgata tgggtgtcgc cagacgggag attagcgctg
     4681 actttcaatg atcgggactg gcctggcgcc agataccagt gggtcgtatc tgaagatcca
     4741 gccgcctcaa cactgccagg cgcgtaggta acgtggatat tatcccgcgt aaaatcgaac
     4801 tcctggggct ggtcggatgt gttggtgtag gtgaagttga taaacgtgcc gagggggaag
     4861 aacgtcacgc tcattgttac tgtcacctgg agcgtcgcgc catcggcata ccaggtctcg
     4921 ccattcttga gcaccgtgcc agccgttgtg agcggatgcg gtgtgggcgt gggtttgggc
     4981 gtcggcgtcg cggtaggggt aggaatcggc gtcggggttg gcgttggtcc ttcggttggt
     5041 gtgggaacag gcggacttgt tggaccagcg ggcgccgcgc cctgactttg cgtgttcgcc
     5101 gaaggtgctt gctgctgatt actcgccaca accggcgccg gctgggtcac cactaccgtc
     5161 atcgggatct gctgtactac cacccgcgcc gttggcgtct ggtacaccgg gagtggaatg
     5221 cccgcggcgg ttgccttagt catcacgaac ccacccaaga caatgccgac aaccaaccca
     5281 gtaatcagtg cccgattaac cgtccaagca aaacgaatcg gccccatctg cggcaatcct
     5341 ttcctgtatc taattgtctc tctgtaatac tggtggggag cacggcgcgg tgggctaact
     5401 ccctaattgt ctggccgcgt gcccgatcaa cgacgtgtcg cccccgtgcc gggctctcga
     5461 ggtgtgacct gagagaagcc agtcgcgcac atcgcgaccc aagagagacc tgtccgcctc
     5521 ggagccgtgc gactcggata atgagaagag gaggcggtcg tgctcacgat tggagtggac
     5581 gcgcacaaac gggtgcatgc ggcggtggcg ctcgatgaca atgggcacga ggtcgcgccg
     5641 tggcgtggtc cgaacagtgt tgacggctgg cggcagctag caggatgggc agcggcgttg
     5701 ggaacgccgt gtcgctaggg gattgagggc agtcggacgt atggccgcgg cctcgcgcag
     5761 ttcctcgtcg cggcgggtgc cgaagtctac gagatcaatc cgcgctggac ggcagcgggc
     5821 cggtggcgtg cgcggcgacc ggacaagaat gatcggcgcg acgcccaggc catcgcgctg
     5881 ctggtgtggc gcgaggcggc ggaattgccg cgggtggtcg ccgatgacga gacggccgtg
     5941 ctggacctgc tggtgaccga gcgcgaggat gtcgtcgcgg agatgacccg gctgaagaac
     6001 caactccacc aactcctgct ccagctcgac ccggagtatg gtgcccgcct gccgagtcta
     6061 cgtacgcaag ccgggctgca cgccctcgaa gggtacaccg tgaccgaccc gactcccgtc
     6121 cagcagcagc gtgctgggac agtccggcgg ctcgccgcgc gcctacggct cgtgacggag
     6181 caagcgaagg ccctcgccac ccagatctgg accctcgcgg cgccgcccag cccgttcctg
     6241 gcgccgcagg cgggccacta acttggccac cggcgacgac gtcccggggt cgccctggtc
     6301 gtggatcgcg cgccaccgtt gcgcggcgtt ccggcagccc tcgccccagc gccgcatcag
     6361 gtagtccagg taaggggcgc gcataggctg ccgcgcccgg cgggtcggct gcttgaccgg
     6421 gggcggctcg ggcatgtgca ggtagcgatc gaccgtgcgc cggccgaccc cgagtgtccg
     6481 ggcgatgtct ttgctggaca ccccttgcgc gtggagcgcg tggatccggc gataccgcgc
     6541 gatacgcact tgatggcggt gctggctcat ctccgccagg cgctgccgcc aggggggcag
     6601 ggcctcctgc tgcgtcgctg cggccgccgg ctggccggca gcgggcggct cgtcctctca
     6661 gtgaacctgg cgcaggaccg ccgtcttggt gaagaggaac ttctcgaccg cgtcgccgag
     6721 gttgcggagc agctggcagc ggtcgacgac ctggcaggcc tgcggcgccc cctgggctgc
     6781 cgccaccgcg tcggcgtcgt accggtcgcg cacggcgacc tcgctgctcg gccgggcggc
     6841 gagccactgc ccgaccgtat cggccgcccg gccgggcagc acgtcaagag ggcggcggcg
     6901 ctccaaatcg acaatgagcg tgccatatgt gagcccgcgg cgaaacgcga agtcatcgat
     6961 gccgaccacc cgtggcgcgc cggtgtcggg cgtgggcgcc cccgcggcga ggcgcaggat
     7021 ggtgtcgtgg ctgctggcga gaccgagccg cgcggccagg cgcgccccag cacggccacc
     7081 cagcgcgagg ccaagctgcc gtaggtcggc ctgctgctga atggtgcggc gcgcataggg
     7141 tgcagccagg tcgggaaagc gctcagcgaa gatgcgccgt gggcaggcca cattggcgca
     7201 ggagaagcgg cgcacctgca ggacgaggaa cacccaccgg ccgcccacgg gccgatccac
     7261 cacggtgcgc tgatagcggc tctggacgcg ctcggagcgc tcgccgcaca acggacaaca
     7321 ggcagcgtcg ccgcaggtgg ccgcgtgtac ggtgatcgcg tccgcagtga tctgtactag
     7381 ttcgatctgg aagcccataa gatgcggcaa ggcggtcgac agctgcacgg cgctctctca
     7441 gtcgcggtcg ccatgcccca gtggcctggc ccaccacagt tgtatacccg agaagggccg
     7501 aacgtgccgc agcgagcgtg cgtcagaccc ccgatgtcgt ggccgggcgt gcaccgcagt
     7561 gcggcactac cgaagccaac ggtggagcgg ttatctcccg acgatcccgt agcggcggca
     7621 gcgctacggc cgcaacgtcc cggcgaagac ctgccaggcc agcgccgatt tcgcgacgag
     7681 gctcaggatc atgtacgcgc gttccccgaa gaggtagtcc cgccacgggc cgatctgctt
     7741 atactgcagg tacatgttca cggcgaaaca gttgaagcag aggaagatcg agacgtagat
     7801 cccgtagacg aaggcgggaa tctccccgcc gccgtagcga ctcgacgcca agaagtagag
     7861 gcccaccacg aaccagggca cgaggcccgc gaggcagccg acccagaagc tgctccaccg
     7921 ccgcggcgcg acgccctggt tgtccagctc catcaccagc cccagcaggt tcatgaccgc
     7981 gaccaggccg aagatcagga ccaggctgcc gaggtcatag accccgacca gcagcgcgat
     8041 gcccaccatc atcaccgagg ccgagaggct atactcgatc caccgggccc ggttcagccc
     8101 cagagccaac tcccgctcgt accggccgcg atacagcgtc gcgatagacg cgtgcgccag
     8161 cgccgagagc agcaagaagc cgaccatcag ccaggccagg ggcacccggc cgatctgctg
     8221 gatcgccggg tccagccgct gcgtctgctc gttgaaggcc aggtaactca cgatgatcgg
     8281 caggcgaaaa tcctggctca gcagcagcat cgccagcgcc tgcgcgccgt gcaacccggc
     8341 catgaccaaa ttgaaggtga agaacggccg cgctccggcg ctcgtccgac ccggtggtgc
     8401 cacccgctcg acttccgccg atactgctcg catacctcac cccctttacc agacactccg
     8461 tcaccgccgt gcgcccaccc gttggcgagc ctaacagccc ggtccacgcc tgccgcgcgg
     8521 caccaggacg ggcaagttca tggccctgcc tctcgcgcag gcgcggcgcc cgtgtcactc
     8581 gcggcacgcc ggtccagtgt gccgatcatc gcaccgccag gtaaaccgcc ggcttgcggc
     8641 gcgtcaacgg gcggtaaatc agtgcggact ctggcgcgcc gcgatccggg cagggtcgcc
     8701 gcatcggcgc ggagcggggt gtcggcgggt gcgcggcgtc gccgggcctg gaagggcttg
     8761 cgccgcgaag ctacggttcg cgaagagccg tcaatcccac atgtcgcgcg cgaccgagcg
     8821 gaccgatggt tgccacaccg gcgtgagcat acccatgccg tgccagcgcc ccgcgccccg
     8881 ccggttgccc ggcggccgtt cgctcgacgc tcaccctggc ggcaccggct gcagccgcac
     8941 gtagtggatc acggccagcg ggtagcagtg gaacaggaaa ccggaataag cgcgctggcc
     9001 gtgccgcgcc gtcctcggcg gccgggtcgt gtagcgggcg gagtgccccg tgcgccaggg
     9061 gctcggccaa ggcgaaagtg tagcgcccga gcaggtgatg tgctcgtggc tgagcgggga
     9121 gaggcgcgcc acatcctccg ggttgaccag cagcccctcg gcgcgcagcc gcgccagcgc
     9181 cagcccgagg tagcgcgtgt tccacagcgc cagggcgttg accaccagcc cgagcgcgcc
     9241 gagctggtct tcctgcccct cgcggtagcg ctggcgcagt ccccctttct gcccgtggaa
     9301 gatggcgcgc gccaggctgt ggcgactctc gccacggttg agttgcacca agatgcggcg
     9361 gcggtaggcc gcgtcgtcga tgtaggccag cagtgaagtt gcctcacttc tgcggacagc
     9421 tgatgatacg gtccgaggag aggaggtgtc ccgatgccat ggacccaccg cccctacccg
     9481 ccggagttcc gggccgaggc cgtgcgcctc gcgcggggca gcgagcggtc gatcctcgcg
     9541 cttgccgttg acctcgggtc tcggccgagg cgctgcgcca ctggctgcgc caggccgacg
     9601 gcgacgacgg ccgggggcaa cccggcgact tgaccaccga cgagcgggcg atcctccgca
     9661 acgcggcggc ctcctgcgcg caggagaccc tgtgcgccgc tctcggtgca gccacgcggc
     9721 gcgggccacc tcgccgatcg ccctgctgtg ccgggggcgg caggttgccc ggtccggcga
     9781 cgacgcctgg acgcgccgcg gggccgcagc gcgcgcccgg cgggacgagg cactgccggc
     9841 gcccatcgcc gccgcccacg cgcgcagccg gcggaccgac ggggcaccgc gcctccacgc
     9901 ggcgttgcgg gcgcagggcg tgcgctgcgc gcggccggcc tggccggctg ccaccggcgc
     9961 cggcggacgc gcaccacgct cgccgacccg acccccacgc cggcgccgaa cctggtcgcg
    10021 cgcgacttcg ccgcacctgc cccggaccgc ctctggatcg gcgaaatagt ctgtacgcaa
    10081 atgacaaaag cgcccctgct cgccgcgcgg gccggtcggg atcacgtagc ggatctcgac
    10141 ctcgccatcc gtcacgatga cgcgggcgat gagcagttcg acgagctggc gctttcgctc
    10201 gaaggtggcg tcggccagcc ccgcccggac ccggcggcag aaatcctcga tgcccgccgc
    10261 cagcccggcc acttcggcgt gccgatccgc ctgtgccgtc agttgctcct cctgctggtc
    10321 gagcgctcgg aggtgctgct cgagatcctg gcggcgacgc tgatactccg gcagcgggat
    10381 caccccgccg acataggcgt ccgtcagccg ttccaattgt tgcctaagac gctgccgggc
    10441 gcggccgagt tgcgcgcggc ggtcctgcag ctcctgtggg agccactggc ccccgtgggc
    10501 gcgctccagc gcctgggtga tgctctccgg attggtcagc acgtcgcaga ggtcgtgcca
    10561 gactagatcg tcgagctgcc cggcggggct gaagcgcgcg gggcacttcg cctccttgcg
    10621 cgtgtagatg gcgcggccct tggccgcgca gacgtagtag ccgtagcccg ccgcatacca
    10681 gcgcccgccg caggacgact ggcagaggcc gcagctgacc agggcgcgca gcaggtagcg
    10741 atgcgccgtg ttgttgcgcg cggcgaagcg ctgattctgc gcgagcttgc gttgcgccat
    10801 ggcgaactcc tcctggctga cgatggccgg gatcgccgcg accgcgatcc actcctccgg
    10861 cggcagctcg gtcagcgagt cgtgcggccg accgaggggg tgggtcgccg agcggcggat
    10921 gcgcgccggt cgcgcgcggt agcgcccggc gtagagctgc ccggtgtagg cgggctggcg
    10981 caggatcgcc cgcagggtcg ccagccccca gagccgcttg ccgctcggcg tcgggatccc
    11041 ctgcgcctgg aggtgctttg ccagcccgcc gaggctggtg cgctccttga tatagtaggc
    11101 gaagatctcg cgcaccaccg ccgcctcgcc ctcgtccacc cacacccccg ccgggtcgcg
    11161 ggggcggtcg gcaccgaggc ggtagccgta gggcgggcgc gtccagggga taagaacgcc
    11221 ggcgcggtac ttctgctgcc gcccccggcg catccggtcg gcgatcagcg tgcgctcgta
    11281 ctcggccacc gccccgcgga tctgcaagag gaggcggtcg tgcgggttct gccccatcgg
    11341 gcggtcgagg aactccacgc ggcagccgcc gcgctccaac tcgtcgagga cgatcatctg
    11401 ctggacgaag ttgcgcgcca gccggtccgg ctcggtcacc agcgcgcagt cgcactcgcc
    11461 gcggcgggcg gcgtcgcgca ggcggtccag ccccggccgg ttcaggtcga cctcgctgcg
    11521 gccatcatcg cggaagacat gctccgggga caaggtccag ccttgtgcgc tgacatgcgc
    11581 ccgcaaccgg gccagctgct gctcaatcgt ctgcgcctgc gcctggttcg ccgtcgagac
    11641 ccgggcgtag atcgccgcgc gcacgctcat gctgcacctc cgccgcttgg caggtcgcgg
    11701 cgctgctccg ccgccccgcc tcgtcctcga gtgcccatcg caggaggtgc tggtacgctt
    11761 ggtcccaccg ccgctcgccg tccgggtggg actggaccgt ccgccgcgac tgccactggc
    11821 gtttcatgtt gccccccgcg atgcgtcgac tcatccaggg ggcagtgtgc ggcaacccgg
    11881 gcctgccgct gccaggcgac ctcaacccgc gaccacgcgg gttgcaggcg accggtccgg
    11941 ctcaggcaga atttgtcgtt tgtgatcagg ccattgacag cacctacgtc gccacgcagg
    12001 agggctggcg ctacctggcg gtgcgcctgg acgcccacgc gcggcgggtc atcggctggg
    12061 cgctggccga ccacctccgc accgaactgg ccctcgacgc gctcgggatg gccctcgagt
    12121 ggcgccgccc gggcgcgggc ctggtccccc acaccgcccg cggcggccag gacaccgccg
    12181 gggcctaccg ggcggcgctc gccgcccgca gcgtgaccgt ctcaatgagc cgcagcggcg
    12241 cgtgccgcga caacgcgctg tccgagcgct gcgtcgccac ccgcaaggcc gagatcgccg
    12301 acgcgcaggt ctggctgagc cgggccgcgg ccaggacggc gattttcgcg tggatcgcgg
    12361 tctggtacaa ccggcagcgc cggcactccg cgctcgccga ccagtcgccc gcgtgctgcg
    12421 aggaggaggt cgtgttgtgg tcttgccccg ccgcctcgcg atacgctgtc cacgaaatcg
    12481 gggcaacctc agtttgcaac ccaggctcgc cagtaccagc taccagtgcc atgatcgccg
    12541 gcaccggcac tgcgttacgc gccagcagcc ggacctgtca cagtagaacg ttcgggcgta
    12601 cgcagttgcc tccgccgtgg aggagagcgt gtagcgtgga cgggcgaggt ggaggcggat
    12661 ggcctcgcgg atgatagcgg tcttggcttc ccaccggctg atgccggtga cggggctgtg
    12721 ctgcttgagc tgtaagaagg cacgaatagc gcagttgatg tagatgcgct gcgcccgtgc
    12781 ggcccggact tccgccgttt gacgccgcac tggtagaccc ggaggccccc gcgcactcgg
    12841 caggcttgcg gcgcagcagt ttgccctgct ggccaactat tccttgtctc cgtccaggtg
    12901 gacgatcagg aaggccagga cgaagccgtc gtcttacaga tggacgtggc gccccgctgg
    12961 gcggatggcg ccgtcatcga gcgggcggtt gcggatgcgg ttggcgttga gtggtgtgac
    13021 ccacgccagc cgtggccccg gattgctttc aggttctcca ggcacgcata ccaacgaccg
    13081 aagatgaccg acgc
//
LOCUS       DASUBI010000162         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_38645, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000162 DASUBI010000000
VERSION     DASUBI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38645"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..315
                     /locus_tag="VFU72_03100"
     CDS             67..315
                     /locus_tag="VFU72_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEU5422503.1"
                     /translation="MDQPQVTLYTQAGCVESAQVRTWLQRHGVPFHERNVSADPDAAR
                     ALAETGIFATPLLVVGGYKILGFRPAEIEEALRASGVI"
     gene            complement(493..738)
                     /locus_tag="VFU72_03105"
     CDS             complement(493..738)
                     /locus_tag="VFU72_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422504.1"
                     /translation="MMDGYHWEQMGNGMGSWGWVGAIAMAVFGIVVLALAVSVIVRLA
                     SNAPTRHDTEPSGDRAEALLRERFARGEIFEAEYRQA"
     gene            1065..1193
                     /locus_tag="VFU72_03110"
     CDS             1065..1193
                     /locus_tag="VFU72_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422505.1"
                     /translation="MALRSDVHAASLLRRGLRLEYATLGWNVVGVVAGLAGFGPAR"
     gene            1204..1611
                     /locus_tag="VFU72_03115"
     CDS             1204..1611
                     /locus_tag="VFU72_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422506.1"
                     /translation="MIGRWPRHGSPHAIPLRVSLVLTLLVLAHFTVMHILDLSAVPAV
                     TRAQSTWAVMADHRVDIPAHSITEQAVCHAWDGCSLLTIRPDSAAIPLPFALLASVLL
                     VLGFILARSTPRLACLPLPRGPARQALLQRFTL"
     gene            1676..2233
                     /locus_tag="VFU72_03120"
     CDS             1676..2233
                     /locus_tag="VFU72_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5422507.1"
                     /translation="MRRVIVPASLLVLALVLVACGSGNSGTSETSEMTGTGTSHSSMA
                     GMAGMSATVGSSDHGQRIAEAAPADQASRTVTISAGDKLAFDPAAVSVKLGETVVFTV
                     TNTGQMAHEFTLGTAAFQEEHEHEMKSGMNMGSVPPDEAAFVFGLAPGESKTVAYRFS
                     QAGQLIYGCHVPGHFTSMRGTITVR"
     gene            2452..2886
                     /locus_tag="VFU72_03125"
     CDS             2452..2886
                     /locus_tag="VFU72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEU5422508.1"
                     /translation="MSRHALSTLGLIVLAALGLALGGRAPNMPVRDLPATMTAPAGVL
                     PLAVERAGPSTVAAYRAALTHPDVLASVPCLCGCQQSLGHRNNLDCYIAGAANGSVTY
                     SMHGVGCAVCQEITRIALEGARQGLSGPELKELVLSHYGVQS"
     gene            2915..3280
                     /locus_tag="VFU72_03130"
     CDS             2915..3280
                     /locus_tag="VFU72_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422509.1"
                     /translation="MTQSDEKVSRRAARRRRQERARGRRTVLLAVLGLAVVGVLALAG
                     FLAAGALSSGGAAKERAAVSWSTSAYSGGPRLAVDRTTVDDGAVPYGREVNATYRLKN
                     VGDQALNLAKASVEALEGC"
     gene            3305..3463
                     /locus_tag="VFU72_03135"
     CDS             3305..3463
                     /locus_tag="VFU72_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422510.1"
                     /translation="MTLQPGQETTLSVQYSMHQGMGGPHRFGITVPSNDPVQPKLTLY
                     ALASYPKP"
     gene            3775..4182
                     /locus_tag="VFU72_03140"
     CDS             3775..4182
                     /locus_tag="VFU72_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422511.1"
                     /translation="MFLAMLVGMAAGALALVLIFSTVLASNVQGMTADEVLARYPVLI
                     CLLVAAGMVATMVGWMRFRGMSWRPVTEMAAAMVVPLVPIFGLLWLDVLAGASACSVY
                     CIAMIPAMLVAMLLRVDLYTASHAAGASTRHAG"
     gene            4489..5178
                     /locus_tag="VFU72_03145"
     CDS             4489..5178
                     /locus_tag="VFU72_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422512.1"
                     /translation="MVDDEPALAELVASYLRREGFTIQTALDGTTALELARTQAPDLV
                     VLDLMLPGIDGLEVCRRLRQFSQAYVLMLTARGDEVDKIVGLTVGADDYLTKPFSPRE
                     LVARVHALLRRPRGVNGAGTDAERVDRFGSLVIDYGAHEVTRHGQPVRLTPHEFALLA
                     TLAARPGQVFSRDQLLARVWGDDFCGDEHVVDVHLSNLRRKLEGDASEPRSIETVRGV
                     GYRFRKFSKAP"
     gene            5178..6305
                     /locus_tag="VFU72_03150"
     CDS             5178..6305
                     /locus_tag="VFU72_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422513.1"
                     /translation="MRSRWSGARLQLKLLFSYLLVLVAALGAVALGMRLIAPSLFDQI
                     LMRHMDSGMMGGAMTLAMRRATDDAFQTTLRQSLLLALALASLVAVAVSVFVTGRITT
                     PIRRMALVSQRIARGDYGARAEVTARDEIGDLARSLNRMAAELETTEQRRIQLIGDVA
                     HELRTPIATLRGYLEGLQDGVVAPSDALWSQLQEQTGRLSRLVDDLRELSRVESGAAS
                     IALEPVAPDELVRASVAAITPRFAEKGVALSVSLAPNAPPVLADHDRAGQVLANLLTN
                     ALRYTPPGGSVIVSTAPGGSVVWFRVRDTGIGVPQEHLPRLFDRFYRVDPARSRALGG
                     SGIGLAIARALVEAQGGEIRADSPGPNQGSTFSFSLPVAHS"
     gene            6424..6687
                     /locus_tag="VFU72_03155"
     CDS             6424..6687
                     /locus_tag="VFU72_03155"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5422514.1"
                     /translation="MMDGYGWSLMTGWGWIGMTLMMLVWFGLLALLAYALSRAFWGTS
                     RNVASGAGGDDRALLVLRERFARGELTEDEFRTASATLRETAN"
BASE COUNT         1042 a         2307 c         2327 g         1100 t
ORIGIN      
        1 cttgccgtcg agggaagggt gtagcctcaa cctcgtcgat ccgggctgtc gacgaaggga
       61 tcgccgttgg accaaccaca ggtcacgctt tacacgcagg ccggctgtgt tgagagcgcc
      121 caggtccgaa cctggctcca gcggcacggc gtcccgttcc acgagcgcaa tgtcagcgcg
      181 gacccggacg cggcccgcgc cctggccgaa accggcatct tcgccacgcc gctcctggtg
      241 gtcggcgggt acaagatcct cggttttcgc cccgctgaga tcgaggaagc cctgcgggca
      301 agcggtgtaa tttgacgtcc ggaggctgat gagccgcgag cagcgccgcg gcgcgtccct
      361 cttccaccac cacggcgagt ggccaaggga gcgccttcag gtaagccagc gagccggcca
      421 gccacgtctt cgagcgcaca acgctggcat ttgcccttct cgccgctacg gcgccggcgt
      481 gcgcagagcc cgctatgcct ggcgatactc ggcctcgaag atctcacccc gggcgaagcg
      541 ctcgcgcagc agggcctcag cacgatcgcc ggagggctcc gtgtcgtgcc gcgtgggggc
      601 gttggaggcc agccgcacaa tgacggagac ggcgagcgcc agcaccacga tcccgaagac
      661 ggccattgcg atggcgccga cccagcccca gctgcccatc ccgtttccca tctgctccca
      721 gtgatagcca tccatcatct cggtccctca caatcggccc ctggagcgcg gcgtgcccgc
      781 cgctgtcgct gccttgctcc accatgcagc gacagcgtag cgcgcgagcc gtgacaatgc
      841 cgtgacggga agtcagcgcg tgtgacaatg gcgcgagagg tcaccgacat gcccggcgcc
      901 ggatcatggt gcgacgctgc ttgttcggct tccgccgcct gtggcagcat ggcgcgggcg
      961 gaaggcgacg catcgctccg cggcacccac ctcccgaaga accctggtgc gccgcttcaa
     1021 tctcagtccg aagccgcgtc aacgcgaggc gagaagggga agtaatggcg ctgaggtcag
     1081 atgtgcatgc cgccagcctg ctgcggcgcg ggctgcgact ggagtacgcc acgctcggct
     1141 ggaacgtggt cggcgtggtc gccgggctgg ccggcttcgg gcccgcgcgg tagactagcc
     1201 gtcatgatcg gacggtggcc gcgccatgga tccccacacg ccattcccct gcgagttagc
     1261 ctagtcctca cgctcctggt cctcgcacac ttcaccgtca tgcatatcct cgatctctcg
     1321 gccgtgccgg cggtgacgcg agcgcagtct acttgggccg tgatggccga ccatcgtgtc
     1381 gacatacccg cccactccat caccgagcag gctgtttgtc acgcctggga cggctgttct
     1441 ctgctgacga tacggccgga cagcgcggcg atcccgctgc ccttcgcgct cctcgcgagt
     1501 gtgctgctcg tgctaggctt catcctcgcc cgttcgacgc cgcgtcttgc ctgcctgccg
     1561 ctcccccgtg gtcccgcccg ccaggcgctc ttgcagcgct tcacgctatg agtgtcacgc
     1621 tcgaaccacg ctgatcgcgc gttggaccag tgacacatgt agcgaaggga tcactatgcg
     1681 ccgcgtcatt gtgcctgcca gcctgttggt gctggcactt gttctcgtgg cctgtgggag
     1741 cgggaactcc gggacgtccg aaacgagcga gatgaccggt accggtacgt cccattccag
     1801 tatggccggc atggcgggga tgagcgccac cgtcggaagc tcggaccacg gccagcggat
     1861 cgccgaggcc gcaccagccg accaggcgag ccggacggtt acgatcagcg ccggcgacaa
     1921 actcgcgttt gatcccgcgg ccgtttccgt caagctcggc gagacggtgg tcttcaccgt
     1981 caccaacacc ggccagatgg cgcacgagtt cacgctcggc acggccgcct tccaggagga
     2041 gcacgagcac gagatgaaga gtggcatgaa catgggaagc gtcccgccgg acgaagcagc
     2101 cttcgtgttc ggcctggcac cgggcgagtc caagacggtc gcctaccgct ttagccaggc
     2161 cggccagctc atctacggct gtcacgtgcc cgggcacttc acctccatgc gggggacgat
     2221 caccgtgcgc tgaggctggc gaggagtcag agagcgtcgc gctcgcgcgg gccccggagc
     2281 atgctccggg cccgcgccgg cacgagcgct ctgcccgctg actggcgctg atcttaccgg
     2341 ttacacgact ttgtaatcct cttccgtacg gagtcacatc gtcgtcatgt cctacgacct
     2401 acccttgggc catcgctcgc gagcgacggc cgagaagcga agggatctgc cgtgtcacgc
     2461 cacgctctta gcacgctggg gttgatcgtc ctcgcggcgc tcgggctcgc gctcggtggt
     2521 cgcgcaccga acatgccggt gcgagacctc cccgcgacca tgacggcgcc ggccggtgtg
     2581 ttgccgctgg cggtcgagcg ggccggcccg agcacggtcg cggcctaccg ggctgccctg
     2641 acgcacccgg acgtgctggc gtcggtgccc tgcctgtgcg gctgccaaca gtccctcgga
     2701 caccgcaaca atctggactg ctatatcgcc ggtgcggcga atgggtccgt cacctactcg
     2761 atgcatggag tcggctgcgc cgtctgccag gagatcaccc gtatcgccct ggagggggcg
     2821 cggcaggggc tgagcgggcc ggagctcaag gaattggtgc tgagccacta cggcgtgcag
     2881 tcgtagtacg cgttagggcg tagggagagg agctatgaca caaagcgacg agaaggtgtc
     2941 gcgccgcgct gcccggcgtc gccggcagga gcgcgcacgc ggtcgccgca cggttctgct
     3001 ggccgtgctg gggctggcgg tggtcggagt cctggcgctg gccggcttcc tggccgctgg
     3061 cgcgctgagc agcggcggcg cggccaagga gcgcgcggcg gtgagctggt cgacgagcgc
     3121 ctatagcggc gggccgcgcc tggcggtcga ccgcacgacc gtcgacgacg gcgcggtgcc
     3181 ctacggccgc gaggtcaacg ccacctaccg gctcaagaac gtcggcgacc aggcgctcaa
     3241 cctggcaaaa gcctcggtcg aggcgctgga ggggtgctga ccgccgacgc cggtcatcgg
     3301 tcagatgacc ttgcagccgg gccaggagac gacgctcagc gtgcagtaca gcatgcacca
     3361 ggggatgggc ggcccgcatc gcttcgggat cacggtgccg agcaacgatc cggttcagcc
     3421 gaagctaacg ctctacgcgc tggcgagcta tcccaagccg tagccggggc cagggaagag
     3481 cggatggcgg tacagacacg aatgcagccc catggatcga gaggtggtga attaacgcgc
     3541 gaacgcgcaa ccaggctgta acagttctgc gacatctgcc gtcacgctgt cactactttg
     3601 cgatgaccca cgcccgaggc tgagaacgac gccgtgacag acgcagcacg ctctcagcct
     3661 ggaggtgctc acatgaacca cccgagtagc agtgttgggg ctagcccgcg gatgcaccgg
     3721 caaccgatct ccgcatgcag gaagacggcc catttcatgc gacacgcggg ggagatgttc
     3781 ctcgcgatgc tggtcgggat ggccgccggc gcgctcgctc tggtgttgat cttctccacg
     3841 gtcctcgcgt cgaacgtcca ggggatgacg gctgacgagg tactcgcccg gtatccggtg
     3901 ctgatctgtc tgctcgtggc ggccggcatg gtcgcgacca tggtcggttg gatgcgcttt
     3961 cggggcatga gctggcggcc ggtgacggag atggccgcgg cgatggtcgt tccgctggtc
     4021 ccgatcttcg gtctgctgtg gctggatgtg ctcgccggcg cttcggcctg ctccgtgtac
     4081 tgcatcgcga tgattcccgc gatgctcgtg gccatgctgc tccgcgtcga cctctacacg
     4141 gcatcgcacg cggccggcgc ctctacccgg cacgctggat gagccgggag tcggttcgaa
     4201 cccggtgtgg agggatcgcg cccgctgacg gctgagaatc gaccgcagag ccatgtctcc
     4261 tcggcgggtc gggcgctctc gacagacggc tcgatcaccc agcccgccgg atcaggtcag
     4321 cctggtcgtg tgcgccgcag cggtaccggc gacaggcgac ggcagaagag tggagggctc
     4381 cattatctct tggtggacca gcgccagcgt gatcgcgaca ccttctcacg atcacgacga
     4441 cgttcagcag agaggattgc tcggttgact cccccagcac gagtcctgat ggtcgacgac
     4501 gagccggcgc tggccgagct ggtggccagc tatctccggc gcgagggctt cacgatccag
     4561 accgccctgg atgggacgac tgccctcgag ctcgctcgca cacaggcgcc cgatctcgtc
     4621 gtgctcgacc tgatgctgcc cgggatcgat ggcctggagg tctgccgccg cctgcggcag
     4681 ttctcgcagg cctacgttct gatgctgacc gcccgcggcg acgaggtcga caagatcgtc
     4741 ggcctgaccg tcggcgccga cgactacctg acgaaaccct tctcgccgcg cgagttggtg
     4801 gcgcgcgtcc atgcgctgct gcggcgcccc cgcggcgtga acggtgccgg cacggacgcg
     4861 gagcgcgtcg accggttcgg ctcgctggtg atcgactacg gcgcacacga ggtgacgcgg
     4921 cacggccagc cggtacgcct cacaccgcac gagttcgcgc tgctggcgac gctggcggcc
     4981 cggcccggcc aggtcttcag ccgcgaccag ctcctcgcac gagtctgggg cgacgacttc
     5041 tgcggcgacg agcacgtggt cgatgtccac ctgagcaacc tgcgccgcaa gctggagggc
     5101 gacgcgagcg agccacgctc catcgagacc gtgcgcggcg tcggctaccg cttccgcaag
     5161 ttctcgaagg cgccgtgatg cggtcgcgct ggagtggagc gcggctccag ctcaaactcc
     5221 tcttctccta tctgctcgtc ctcgttgccg ccctgggcgc ggtggcgctg ggcatgcgcc
     5281 tcatcgcgcc gtcgctcttc gaccagatcc tgatgcgcca catggacagt gggatgatgg
     5341 gcggcgcgat gacgctcgcc atgcggcgcg cgaccgacga cgcctttcaa acgaccctcc
     5401 ggcagtcgct gctgctggcg ctggcgctcg cgagcctggt ggcagtcgcg gtcagcgtct
     5461 tcgtcaccgg ccgcatcacc accccgatcc ggcgcatggc gctagtcagc cagcgcatcg
     5521 cgcggggcga ctacggcgcg cgggcggagg tcacggcgcg cgacgagatt ggggacctgg
     5581 cgcgaagcct gaaccggatg gccgccgagc tggagacgac cgagcagcgg cggatacagc
     5641 tgatcggcga cgtcgcgcac gagctacgca cgccgatcgc cacgctgcgg ggctacctgg
     5701 aaggtctgca agatggcgtc gtcgcgccat cggacgcgct ctggtcgcag ctgcaggagc
     5761 agaccgggcg cctgagccgg ctggtcgacg acctgcggga gctctcccgc gtcgagtcgg
     5821 gcgcggccag catcgcgctt gagccggtgg cgccggacga gctggtgcgg gccagcgtcg
     5881 cggcgatcac gccccggttc gcggagaaag gtgtcgcgct ctccgtctcc ctggcaccaa
     5941 acgcgccgcc ggtgctggcc gaccacgacc gcgcggggca ggtgctggcc aatctcctga
     6001 ccaacgccct gcgctacacc ccgcccggcg gcagcgtcat tgtctccacc gcgcccggcg
     6061 ggtcggtcgt ctggttccgc gtgcgggaca ccggcatcgg ggttccacag gagcatctcc
     6121 cacggctctt cgaccgcttc taccgcgtgg accccgcgcg gtcccgcgcg ctcggcggca
     6181 gcggcatcgg gctggccatc gcacgggcgc tggtcgaggc gcagggcggc gagatccgcg
     6241 ccgatagccc cggccccaac cagggcagca ccttcagctt cagcctcccc gtcgcgcact
     6301 cctgagcgct cacgatcttg atccaatctt gagcaatctc cgcatcgacc cttgaaccgg
     6361 ggagcctacg cttgagtcag atgacgggac gtccgtcaca agtggctcaa ggaggaaaag
     6421 acgatgatgg atggctacgg ctggagtctc atgacgggct ggggctggat cggcatgaca
     6481 ttgatgatgc tcgtctggtt tgggctgctg gcgctgctgg cctacgcgct gagccgtgcc
     6541 ttctggggca cgagccggaa tgtggcgtcc ggcgcgggcg gcgacgatcg cgccctgctc
     6601 gtgctgcgcg agcgcttcgc ccgcggcgag ctcaccgagg acgagttccg cacggccagc
     6661 gccacgctgc gtgagacagc gaattgacta tgccgttgga gcggcatgcc atcgaaggga
     6721 gcgagtcatg cggcggagat tcacggtgct ggtggcgatc ctggcggtcg ggttgc
//
LOCUS       DASUBI010000163         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_203628, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000163 DASUBI010000000
VERSION     DASUBI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203628"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..995
                     /locus_tag="VFU72_03160"
     CDS             84..995
                     /locus_tag="VFU72_03160"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5422515.1"
                     /translation="MSLPQRVRITEVGPRDGLQNEATHLSTAEKVALIARLAAAGLRD
                     IEATAFVHPRWIPALADAEDVLAALPRKERDQGVCYSVLVPNLKGYQRAAPLQPDEVS
                     LVCSASQGHNRANLNRSTEESLTGLAEVAAAARADGIPLRGAISTSFWCPFDGRTPEA
                     RVVEVARAYAAMGVAEVCLADTLGTADPRHVAGLVPRVRDATGLPVSLHLHDTAGMAL
                     ACVLAGLEAGVERFDAAVGGLGGCPYAPGAAGNLATEDLVGMLHQMGIATGVDEPALL
                     EAAYYLRDAVGHPLPSHRLALAGAQAH"
     gene            1026..2111
                     /locus_tag="VFU72_03165"
     CDS             1026..2111
                     /locus_tag="VFU72_03165"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEU5422516.1"
                     /translation="MSTRQTDPALLGYQTSSVADRMGEAAERFLAALAPDQRAMAVFP
                     LDDDAERRSWHYTPIPRPGLPLARMERHQQRLAHQLVSTGLSRSGYATAATIMGLEST
                     LDLIEGWEREDPGRDPARYYVSVFGTPSASAPWGWRFEGHHVSLHYTIAGGRIVAPTP
                     TFFGSNPAETPLSGTATLRPLAGVEDLARQLVHTLDQEQRARAILAPVAPADIVLANR
                     THVEPGVLPELRDDRQRDFTPAHAEALRYTAEPRGLGARAMSAGQRELLQALLHEYIG
                     RMPDEIAEMERQRLRGALDEVHFVWAGGIERRQGHYYRLQGTRFLVEYDNTQDDNNHI
                     HSVWRDPSNDFGADLLAQHYRRAHGTS"
     gene            complement(2358..>2560)
                     /locus_tag="VFU72_03170"
     CDS             complement(2358..>2560)
                     /locus_tag="VFU72_03170"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU5422517.1"
                     /translation="VCEQRDVKGLYAQARRGEVRGFTGIDDPYEAPESAEITLDTVSC
                     TAQENARRIMDHLRDQGFVRGD"
BASE COUNT          366 a          969 c          885 g          340 t
ORIGIN      
        1 gcgagggcag acacggaggt ctgcccctac agcgggcgac ccgtgtggcg gttgctctag
       61 ggcgctgcga aggagtccga gcgatgtcgc tcccccagcg cgtgcgcatc accgaggtcg
      121 gaccgcgcga cggcctgcag aacgaggcga cgcacctctc gacggcggaa aaggtggcat
      181 tgatcgcccg cctggccgcc gccgggctgc gcgacatcga ggccaccgcc ttcgtccacc
      241 cgcgctggat cccggcgctg gccgacgccg aggacgtgct ggccgcgctg ccgcgcaagg
      301 agcgcgacca gggcgtctgc tacagcgtcc tggtgcccaa cctcaagggc taccagcgcg
      361 ccgcgccgct ccagcccgac gaggtctcgc tggtctgctc ggccagccag gggcacaacc
      421 gcgccaacct caaccgctcg accgaggaat ctctgaccgg gctggccgag gtcgcggccg
      481 ccgcccgcgc cgacggcatc ccgctgcgcg gcgcgatctc gacctccttc tggtgcccct
      541 tcgacggccg cacgccggag gcgcgcgtgg tggaggtcgc tcgcgcctac gccgccatgg
      601 gcgtggcgga ggtctgcctg gccgacacgc tcgggaccgc cgacccgcgc cacgtggccg
      661 ggctggtgcc gcgcgtgcga gacgcgaccg ggctgcccgt cagcctgcac ctgcacgaca
      721 cggccggcat ggcgctggcc tgcgtgctgg ccgggctgga ggccggggtc gaacgcttcg
      781 acgccgccgt gggcgggctg ggcggctgcc cctacgcgcc gggcgcggcc gggaacctgg
      841 cgaccgagga tctggtcggc atgctgcacc agatggggat cgcgaccggc gtcgacgagc
      901 cggcgctgct ggaggccgcg tactatcttc gcgacgcggt cggccacccg ctccccagcc
      961 accgtctggc cctggccggc gcgcaggcac actgagcgtc gagccgcatc acgaaggagc
     1021 gcgccatgag caccaggcaa accgaccccg cgctgctcgg ctaccagacg agctcggtcg
     1081 cggaccgcat gggcgaggcc gccgagcgtt tcctggccgc gctggcgccc gaccagcgag
     1141 ccatggccgt cttcccgctg gatgacgatg ccgagcgccg ctcctggcac tacaccccga
     1201 tcccgcgccc cggcctgccg ctggcgcgga tggagcgaca tcagcagcgg ctggcgcatc
     1261 agctggtctc aaccgggctg agccgcagcg gctacgctac cgccgccacg atcatggggc
     1321 tggagtcgac gctcgatctg atcgaaggct gggagcgcga ggaccccggt cgcgatccgg
     1381 cgcgctatta tgtcagcgtc ttcggcacgc ccagcgcgag cgcaccctgg ggctggcgct
     1441 tcgaggggca ccacgtctcg ctgcactaca ccatcgccgg cgggcgcatc gtggcaccga
     1501 cgcccacctt cttcggctcc aacccggccg agacgccgct cagcggcacg gctaccctgc
     1561 gcccgctggc cggcgtcgaa gacctggcgc ggcaactggt ccacaccctc gatcaggagc
     1621 agcgcgcccg ggccatcctg gccccggtgg ccccggccga tatcgtgctg gccaaccgca
     1681 cccacgtcga gccgggcgtc ctgccggagc tgcgcgacga ccgccagcgc gacttcaccc
     1741 cggcgcacgc cgaggcgctg cgctacaccg ccgagccgcg cggcctgggc gcccgcgcca
     1801 tgtcggccgg gcagcgcgaa ctgctccagg cgctgctcca cgagtacatc ggccgcatgc
     1861 cggacgagat cgccgagatg gagcgccaac ggctacgcgg cgcgctggac gaggtgcact
     1921 tcgtctgggc cggcggcatc gagcgccgcc agggacacta ctatcgcctg cagggcacgc
     1981 gcttcctggt cgagtacgac aacacccagg acgacaacaa ccacatccac tcggtctggc
     2041 gcgacccctc caacgacttc ggcgccgacc tgctggccca gcattaccgc cgggcgcatg
     2101 gcacgagctg aagctgtgga gatgcgtccg tgctccgtgt gacgggggaa ggagcggacg
     2161 ggcgaggacg tcacctcgtc atcgcacgcc ccctgcgaca cccgtcattc tgagcttgtc
     2221 gaaggatcca agccgcgcct gccggccaac gaagaagggc gggtttcgaa cccgccccga
     2281 cattgtcgtg ctccccttct ccccatcgga tccttccgtg ggttcggaga aggggtggag
     2341 agacgaaagc tccccgccta gtcgccgcgc acgaagccct ggtcgcgcag gtggtccatg
     2401 atgcggcgcg cgttctcctg cgccgtgcag ctgaccgtgt cgagcgtgat ctccgcgctc
     2461 tccggcgcct cgtacgggtc gtcgatgccg gtgaagccgc gcacctcgcc gcgccgcgcc
     2521 tgggcgtaga gccccttgac gtcgcgctgc tcgcagacct
//
LOCUS       DASUBI010000164         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_173371, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000164 DASUBI010000000
VERSION     DASUBI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173371"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFU72_03175"
     CDS             complement(<1..414)
                     /locus_tag="VFU72_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422518.1"
                     /translation="MAQSATTRYRFATDAQVPLEATFDGGRLTSDGGLPWLAEADAEL
                     GLCAAYARCLPDWRTDPARAQHSLEALVRQRVFQLACGYADQDDADSLRTDPLFKLVC
                     GQRPDSGHDLASQPTLSRLDNCVDRLHCRRLSEALG"
     gene            596..1852
                     /locus_tag="VFU72_03180"
     CDS             596..1852
                     /locus_tag="VFU72_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422519.1"
                     /translation="MGDVQAEERTGAAAGATAAPQAASTARSGTLNLKAVLTMAFAHF
                     SHDLYGSFIAVLIPPIQDKLGIPLSVVSLMSPAQQFPSILQPAVGAVADRVGRRWFVV
                     LAPSVAAVSVSLVGLAPHFGIVLLLLLTAGLASAAFHPPAVTLAGEFSGGKTGQAMGW
                     FMAGGQLARTLGPLLITAAIAWFTLEGSFVVMIFGLLASVLLFLTLDTREVDTQSKAK
                     VQVPLRPLLRARWSWITSLVLVGFVRSVSSVPFTLFLVKLLEDQGHSAWYGGFSLSVL
                     FGAGVFGGFLGGTLSDQYGRRSILALTSVISPPLLYLYLWQSEHLIPAIALLIVNGVV
                     LSALRPVQLAYIHDMVPEAPGPANGLILAFQFVGQSVAAFAFGAFADSIGLVSAFWWV
                     PPTALLALPLIALLPGRRHIAELHRV"
     gene            complement(1876..2211)
                     /locus_tag="VFU72_03185"
     CDS             complement(1876..2211)
                     /locus_tag="VFU72_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422520.1"
                     /translation="MNMTYRKPQGARELEARSRARERGVRVRVRQPQRHYTSRSQTYS
                     NVTYHLEKAPEGWACSCEGYSYTGVCKHLAALERRSEREGWRFGKVAPVYPIVPAPRL
                     ALVDPSLFA"
     gene            2518..3753
                     /locus_tag="VFU72_03190"
     CDS             2518..3753
                     /locus_tag="VFU72_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422521.1"
                     /translation="MSPAVRRAVLMLAVMSAAFGMSMAIQENIVTNYFDQVLGLRGPQ
                     FGYITAIREIPGFLLILLTAVFYRMSVPRLTAMALVLVTVGYCFFGLSNSFWTVAPWV
                     VISSMGYHTVLQTQYALGMSLTQQNRSGRILGWMSAVNAGGGLLAMILVLVTFHYGWL
                     SFRPTFVIAGAIALVAAFAIFRFPTMQDGVTTVHLPRRERIVLRRSYRLYYYLALLDG
                     ARQQIFFSFGLWVLVQEFGMTVPVISALLLLVRCSGIVVGPWMGRLIDARGERAMLGI
                     INVSYVVALGGYAFFHNAIIAAACYAVYSLIFPLSSVGSATYLRKIAVSDEVAPSLAM
                     GVTLQHAAAIVVPVSTGFILNFVGYQVPFLIACGFAVMTILLTRRLDPETQRSPGRIA
                     EDARLAMREVQPETAAAVG"
     gene            complement(3968..4975)
                     /locus_tag="VFU72_03195"
     CDS             complement(3968..4975)
                     /locus_tag="VFU72_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5422522.1"
                     /translation="MAQRTRPRVALIGTGGTIQSLGRDSLDLYEYGANNGVLSATQLV
                     EQVPELASIAEIIPFASWTKASTAVIPSDWLALNEQIHRVVAENPDLSGVVVTHGTAT
                     LEETAYFLNLVLKVDLPVVVTGAQRPFSALSGDARLNLVNAVRVASDPASRGLGVLVV
                     LNDEINAAREVTKTSTYRLETFRTPDLGMLGYADPDAVVYYRRPTRRHFPQTEFDVRR
                     LTDLPRVDIVYAYAGADATAIEAALAAGAQAVVLASFAPAGCSRAQSAALIDAIQRGV
                     AVVQAARVGSGRVLAGSSLRRHGFVVADNLNPQKARVLTMLGLTAGADGEALQRMFWE
                     Y"
     gene            complement(5002..>5617)
                     /locus_tag="VFU72_03200"
     CDS             complement(5002..>5617)
                     /locus_tag="VFU72_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422523.1"
                     /translation="GTILGYVGVMGPQDGVDYLLRALRILVEDLGRREVYCILIGPGD
                     ALDELKALTADLRLEDHVCFTGPIHDPDRLARYLSTADICVDPDPLNSYNDRSTMLKL
                     MDYMAVARPIVAFDLAEHRRTAEGAAVFVPGNDERGFAEALAALIDDPERRERLGRAG
                     RQRVETELAWEYSVVNLLQVYELLLPAARVAPVVHPSSELAKDA"
BASE COUNT          854 a         1966 c         1742 g         1055 t
ORIGIN      
        1 gcccaaggcc tcgctcaggc gccgacagtg gaggcggtcg acgcaattgt ccaggcgcga
       61 gagggtcggc tggctggcca ggtcatgccc cgagtcgggg cgctggccgc agaccagctt
      121 gaagagcgga tcggtgcgca agctatcggc atcatcctgg tcggcgtagc cacatgccaa
      181 ctggaagacc cgctggcgca ccagcgcctc caggctgtgc tgggcacggg cgggatccgt
      241 gcgccaatcg gggaggcagc gggcgtaggc cgcacagaga ccgagttcgg cgtccgcctc
      301 cgccagccag ggcaacccgc cgtccgaggt caggcggcca ccatcgaagg tcgcctccag
      361 cggcacctgc gcatccgtcg cgaagcggta gcgagttgtg gcagactgag ccatgcaaga
      421 gcctcctggt gaccgccgag tcgtcgaaca actcgaccat aaccgcggag gctcttggct
      481 tcaaccccct cgtgaataat ccgggctagc atccttcccg cgtagcagcc gttcgcgaac
      541 tacgtggtgt actatctccg gaagcgattc gatcgcgttg cgaggagatg ggccgatggg
      601 agatgtgcag gcggaggagc ggaccggggc cgccgcgggt gcgacggcgg cgccccaggc
      661 agcgtcaacc gctcgcagcg gcaccctcaa cctcaaggcc gtgctcacca tggcctttgc
      721 ccacttctcg cacgacctct acggctcgtt catcgccgtt ctgatcccgc cgattcagga
      781 caagctcggc atcccgctct cggtcgtctc gctgatgtcc ccggcccagc agttccccag
      841 tatcttgcag cccgccgtcg gcgcggtcgc cgatcgggtc gggcggcgct ggttcgtggt
      901 gctggcgcct tccgtcgcgg cggtctcggt ctcgctggtc gggctcgcgc cgcacttcgg
      961 catcgtgctc ctgctgctcc tgaccgccgg gctcgcctcg gccgccttcc acccaccggc
     1021 ggtgacgctg gccggtgaat tctccggcgg caagaccggc caggcgatgg gctggttcat
     1081 ggccggcggg caactggcgc gcacgctcgg gcccttgttg atcaccgccg ccatagcctg
     1141 gtttacgctc gaaggctcct tcgtggtgat gatcttcggg ctgctggcct cggtgctcct
     1201 cttcctgacg cttgacacgc gggaggtcga cacccagtcg aaagccaagg tacaggtgcc
     1261 gctgcggccc ctgctgcgcg cccgctggag ctggatcacc agcctcgtgc tggtcggctt
     1321 cgtccgcagc gtcagttcgg tgccgttcac gctcttcctg gtgaaacttc tggaggacca
     1381 agggcacagc gcctggtacg gcggtttctc gctctcagta ctcttcggcg cgggggtgtt
     1441 tggcggattc ctgggcggca cgctctccga tcagtacggt cggcgcagca tcctggcgct
     1501 gacctcggtc atcagcccac cgctcctgta tctctatctc tggcagtccg agcatctgat
     1561 cccggcgatc gcgctcttga tcgtcaacgg cgtggtgctt tcggcgctgc ggccggtgca
     1621 gttggcctac atccacgaca tggtgcccga ggcgccagga cccgccaacg gcctgattct
     1681 ggctttccag ttcgtcggac agagcgtggc agccttcgcc tttggcgcct tcgccgactc
     1741 catcggtctc gtcagcgcct tctggtgggt gccgccgacc gcgctgctgg ctctgccgct
     1801 gatcgcgttg ctgcccggcc gccggcatat cgccgaactg caccgcgtct gacgtcgttc
     1861 gtcgccaccc gcctgctacg cgaagagaga tggatcgacc agcgccaggc gcggcgcggg
     1921 cacgattggg tagaccggcg cgaccttgcc gaagcgccaa ccctcccgtt cggagcgccg
     1981 ctcgagcgcc gccagatgct tgcacacacc ggtgtagctg tatccctcgc agctgcaggc
     2041 ccagccctcc ggcgcctttt ccaggtgata ggtcacgttc gagtaggtct gactgcggct
     2101 ggtgtagtgc cgctgcggtt gcctgacacg cacacgcaca ccccgctcgc gagcccgcga
     2161 gcgcgcctcc agctcgcgcg caccctgggg ttttcggtag gtcatgttca tgattcggaa
     2221 ctcctggcta ctagacgccg cgcgatttga cgacgtctga atccgtgatt ataccactct
     2281 cgtagatact cgcgtacgca gcatcacctg ctcaagaaca ccggtcgcag ccttcattcc
     2341 caccaatcgc ctcgtagaga cactccatca ccggaccgca gatgggccgg ttacatcgca
     2401 atccgattcg atcgggcgaa atagtctgat agcgtaaacg cgagatgcga taattcaggg
     2461 gttgcgcgac ctcgtctttc acgcggagaa cccttgatgt ctttcctgga tgaacggatg
     2521 agcccagccg tccggcgcgc cgtcttgatg ctcgccgtca tgtccgcggc cttcggcatg
     2581 tcgatggcga tccaggagaa catcgtcacc aactacttcg accaggtgct cgggctgcgc
     2641 ggcccgcagt tcggctatat caccgccatt cgcgagattc ccggcttcct gctgatcctg
     2701 ctcaccgcgg tcttctatcg catgtcggtg ccccggctga ccgcgatggc gctggtgctg
     2761 gtcaccgtcg gctactgctt cttcggactc tccaattcct tttggacggt ggcgccctgg
     2821 gtcgtcatct cctcgatggg ctaccacacc gtgctgcaga cgcagtacgc cctcggcatg
     2881 agtctcaccc agcagaaccg cagcgggcgg attctgggct ggatgtcggc ggtcaacgcc
     2941 ggcggcgggc tcttggccat gatcctggta ttggttacct tccactacgg ctggctctcc
     3001 ttccgcccga cgttcgtcat cgccggagcc atcgcgctgg tcgccgcctt cgcgattttt
     3061 cgcttcccga ccatgcaaga cggcgtcacc acggtccacc tgccccggcg tgagcgcatc
     3121 gtactgcgcc gctcgtaccg gctctactac tacctggcgc ttctcgatgg agcgcggcag
     3181 cagatcttct tctcgttcgg cctctgggtg ctggtgcagg agttcggcat gaccgtgccg
     3241 gtcatcagcg cgctgctctt actggtgcgc tgctccggaa tcgtcgtcgg cccctggatg
     3301 ggtcggctga tcgacgcgcg cggcgagcgc gccatgctcg gcatcatcaa cgtgtcctac
     3361 gtggtggcgc tcggcggcta cgcgttcttc cacaatgcga tcatcgccgc ggcgtgctac
     3421 gcggtctact cgctgatctt cccgctctcc agcgtcggct cggccaccta tctgcgcaag
     3481 atcgccgtct ccgacgaggt cgcgccctcg ctggccatgg gagtcacgct gcagcacgca
     3541 gccgctatcg tcgtgccggt ctcgaccggc ttcatcctga acttcgtggg ctatcaggtg
     3601 cccttcttga tcgcctgcgg cttcgccgtc atgactattc tgcttacccg tcgtctcgat
     3661 ccggaaacgc agcgctcacc gggtcgcatc gccgaggacg cccggctggc gatgcgtgag
     3721 gtgcaacccg agaccgcggc cgccgtcggc taggccctgt ctgaggaatc ggacgcctgc
     3781 cgtgcgcggc cggcttcctt cctggtccgg tttggcgcgg gagcgagcca gtgaggcacg
     3841 gatccttcga ctgcgctcag gatgacaggg gaaggaatga cgcctaccgg acgaggcacg
     3901 tcgtagtcgt acgccccatc ccctaccctg tcatcctgag ccgcctaacg tgttcgtcag
     3961 gcagccccta gtactcccaa aacatccgct gcagcgcttc gccgtcagcc ccggccgtca
     4021 atccgagcat cgtcaacact cgtgccttct gtgggttgag gttgtcggcc accacgaacc
     4081 catgccgccg cagcgaactc cccgccagca ctcgcccgct gccgacgcgc gccgcctgca
     4141 ccaccgctac ccctcgctgg atcgcgtcga tcaacgctgc gctctgcgcg cggctgcagc
     4201 cggccggcgc gaacgacgcc agcaccacgg cttgcgctcc ggctgccaag gcggcctcga
     4261 tcgccgtcgc gtcagcaccc gcataggcgt atacgatgtc cacccgtggc aggtccgtta
     4321 ggcggcgcac atcgaactcg gtctgcggga agtggcgtcg agtcgggcgt cgatagtaga
     4381 ccaccgcgtc agggtccgcg tagccgagca tccccagatc cggggtgcga aacgtctcca
     4441 gccggtacgt tgaggtcttg gtcacctcac gcgcggcatt gatctcgtcg ttcagcacca
     4501 ccagcacgcc gagcccgcgc gaggccggat ccgacgccac gcgcaccgcg ttgaccagat
     4561 tgagccgcgc atcgccgctc agcgccgaga agggacgctg tgccccggtc acgacaacgg
     4621 gcaggtcaac tttcaacacc agattgagaa aataggccgt ctcctccaga gtcgccgtgc
     4681 cgtgcgtcac gaccacgccg gacaaatcgg ggttctcggc caccacgcgg tggatctgct
     4741 cgttcaacgc cagccaatcg cttggtatga ctgccgtgct cgccttcgtc cagctcgcga
     4801 aggggatgat ctcggcgatc gaggcgagct ccggcacctg ctcgacgagc tgcgtcgcgc
     4861 tgagcacgcc gttattcgct ccatactcgt agaggtcgag gctatcccta ccgagcgact
     4921 gaatcgtacc gccggtgccg atcagcgcca cccgagggcg cgtgcgctgt gccatcatgc
     4981 tctccaactc accgccgtcg ctcacgcgtc cttcgccagt tcactggacg gatgcaccac
     5041 cggcgcgacg cgcgccgccg gcagcagcag ttcatagacc tggagcaggt tcaccaccga
     5101 gtactcccaa gctagctcgg tctcgactcg ctgccggccg gcgcgaccga ggcgctcgcg
     5161 gcgctcggga tcgtcgatca gcgccgcgag cgcctcggcg aagccgcgct cgtcgttgcc
     5221 gggcacgaag accgccgcgc cctcggccgt acgccggtgc tcggccaggt caaaggccac
     5281 gatcggcctg gcgaccgcca tgtagtccat cagcttgagc atggtcgagc ggtcgttgta
     5341 cgagttcaga ggatcggggt cgacgcaaat gtccgcggtc gagagatagc gcgcgagccg
     5401 atcgggatcg tggatcggac ctgtgaagca gacgtgatct tccagccgca ggtccgcggt
     5461 cagcgctttc agctcgtcga gcgcgtctcc cggtccgatc aagatgcaat acacctctcg
     5521 ccggcccagg tcttcgacca ggatgcgcag cgctcgcagt aggtagtcga cgccatcctg
     5581 cggccccatc acacccacat agccgagaat cgttccg
//
LOCUS       DASUBI010000165         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_472557, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000165 DASUBI010000000
VERSION     DASUBI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472557"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFU72_03205"
     CDS             <1..816
                     /locus_tag="VFU72_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422524.1"
                     /translation="LALGLVLAEVPLARPVYVELNPDWGNARQLLPRANRATVVDLLD
                     AYAPIERTGIGLLQSYITMWSRLPVLTVPTDPDQAAALGAADYERVLQLLGQYYNVIL
                     LDCGTGYADTLTQFGIQRADHLVLLSSPDHGTVRTALESARYLASTRYERTFADRLAA
                     LAAGGTADIRARQLAAVTLVVNQHGRPGAGIPADLGKVRRAASALNAVLTVPHSATLA
                     TLFDDQTLTLENLPRPVRRALKSLWVAVLDQLVAQQREAGVAAGSAPAAQEGS"
     gene            816..1052
                     /locus_tag="VFU72_03210"
     CDS             816..1052
                     /locus_tag="VFU72_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEU5422525.1"
                     /translation="MAAFFYALYNNLVAPLALALGVVAFAAASVQFLLGHREGVERLL
                     RVAAGLFLLGFAPVIVQMIWSAVLNSGGGVASGR"
     gene            1065..1418
                     /locus_tag="VFU72_03215"
     CDS             1065..1418
                     /locus_tag="VFU72_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422526.1"
                     /translation="MRTTNRTPRRLDDPLRIGPFTLAQWGVLLLATALVWLAARELAF
                     IAWRARLMLGALVVGLAGGLARTGYGGSLLEQPRKAWHTLTTPREYVARPPRQGPLVL
                     ELVDMAPAEEEPPDA"
     gene            1411..3933
                     /locus_tag="VFU72_03220"
     CDS             1411..3933
                     /locus_tag="VFU72_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422527.1"
                     /translation="MPSPLSRAGSAADLLPLLDLAEDHFVTTTGRLGRVLACTGLNLG
                     IASAATADQVAAHFAGVLDYLPTGCRVQFVALNRPLRAAAWVPRHLAEYRRHAPSRLH
                     EAVDLLADAYQTALGAGAVPDLHFYAVLTLPGAPAPHRRVAQRARVRRRLLRDRAAWQ
                     RALGDLDRADQQLARTLGALGIQAAALGRQGTLDLLWQCANPAWSRHAAAPRAEARPR
                     HPADLRALREQLAQSRLTRRHDYLRLDWGYETTLALRALPEETFPGWLGQLTASGVTF
                     RFALHVEPLAKERERRTLTQALKRRHAVLAERQGKGLPPDIEQEESYSEVAGVLRDMA
                     AGDLRTFRIAVFVTVRAPSVEQLQEAVGAVVYALGDAGGTSVDRCLLWQLPAWQATLP
                     LAENPAGMEYRVVTPNLADTLPFLRHGAGTKGGPLVGFAAPSREAVTLDLLDPGLPNP
                     ALVVCGRSGSGKTLFAQAYAFKHVLRGGRVVVLDRSTGHWDALAAAIGDAQVVPVGLD
                     GRCRLNPWQLPDGVREPDHRKLQYLVDLHHLLLGEEGGLDPAAAALVEASCRAVYRAN
                     AAPYERDLAAWLAALAADPEQEAGRRQRAALLADRLGPYTGDGLYARLLDGATAVRAD
                     ALLTIFNFKGLSDRLVPLAMLPLLEYLWTVIADPARPLLLVLDEGWKLLEHPASARFV
                     TEVARTGRHHGLAQLHLSQFPTDYTGGVGQTVLENAAATLLLAQHPHLLDDCQRIFGL
                     TDDERAIVARLGTVKGQAAGAYLRAGEGADSGAVELWVTPTEYWLCTSHPPEVALRAA
                     ALRRHSGDVWTAVRELAAMSPAGRATLGALAQPVTAAAAGGV"
BASE COUNT          520 a         1494 c         1402 g          593 t
ORIGIN      
        1 ctggcgctgg gtctcgtcct ggccgaggtg ccgctggccc ggcccgtcta cgtcgaactc
       61 aatccggact gggggaatgc ccgccagctc ctgccgcgcg ccaaccgcgc gaccgtggtg
      121 gacctgctcg acgcctacgc ccccatagag cggaccggca tcggcctgtt gcagtcctac
      181 atcaccatgt ggagccgcct gccggtgctg acagtgccga ccgatcccga ccaggcagcg
      241 gcgctcggtg cagctgacta cgagcgggtg ctgcaactcc tcgggcagta ctacaacgtc
      301 atcctgctcg actgcggcac cggctacgcc gacaccctga cccagttcgg catccagcgc
      361 gccgatcatc tcgttctcct cagttcgccc gaccacggca cggtgcgcac cgcgctggag
      421 tcggcgcgct atctcgcctc gacgcgctac gagcggacct ttgccgaccg actggccgcg
      481 ctggccgccg gcggcacggc cgacatccgc gcgcgccagc tcgccgcggt gacactggtg
      541 gtcaaccagc acggccggcc gggggccggc atccccgccg acctcggcaa ggtgcggcgg
      601 gcggccagcg cgctcaacgc ggtgctgacc gtgccgcact cggccacgct ggcgactctc
      661 tttgacgacc agacgttgac gctggagaac ctgccgcggc cggtgcggcg ggcgcttaag
      721 tcgctctggg tggcggtcct cgaccagctc gtagcgcagc agcgggaagc cggcgtggcg
      781 gctggcagcg cacctgcggc gcaggagggt tcgtgatggc ggcgttcttc tacgcgctct
      841 acaacaacct ggtcgcccca ctggcgctcg cgctcggcgt ggtcgctttc gcggcggcgt
      901 cggtgcaatt cctcctgggc catcgcgaag gggtggagcg actgctgcgc gtagccgcgg
      961 gcctcttcct gctcggcttc gcccctgtca ttgtgcaaat gatctggtcc gccgtgctca
     1021 acagcggtgg cggcgtggcc agcggccgct gagggaggcg cgccatgcgt accacgaacc
     1081 gcactccccg ccggctggat gacccgctcc gcattggccc gttcaccctt gcccagtggg
     1141 gcgtgctcct cttggccacg gccctagttt ggctggcggc gcgcgaactc gcgttcatcg
     1201 cctggcgggc gcggctcatg ctcggtgcgc tcgtcgtcgg cctcgccggc ggcctcgcgc
     1261 gcaccggcta cggtggctcg ctcctcgagc agccgcgcaa agcctggcac acgctcacta
     1321 ccccgcgcga gtacgtggcc cggccgccgc gccaggggcc tctagtgctc gaactggtgg
     1381 acatggcccc ggccgaggag gagccacccg atgcctagcc cgctaagtcg cgctggcagc
     1441 gcggccgacc tgctgcccct cctcgaccta gccgaggacc acttcgtcac caccaccggg
     1501 cggctgggcc gggtgctggc ttgcaccggc ctcaatctcg gcatcgcctc cgccgcgacc
     1561 gccgatcagg tcgccgccca cttcgccggc gtgctcgact acctgccgac cggctgccgc
     1621 gtgcaattcg tcgccctcaa ccgcccactg cgcgctgccg cctgggtgcc gcgccacctg
     1681 gccgagtacc gccggcacgc gccgtctcgg ctgcatgagg cggtggacct cctggcggac
     1741 gcctaccaga cggcgctcgg cgcgggcgcg gtgccggacc tgcacttcta cgccgttcta
     1801 acgctgccgg gcgcgccggc cccacatagg cgggtggcgc aacgggcgcg ggtccgtcgt
     1861 cgcctcctgc gcgatcgggc ggcgtggcag cgtgcgctcg gcgatctcga ccgggcggac
     1921 cagcaactcg cccgcaccct gggcgcgctc ggcatccagg cggcggcgct cggccggcag
     1981 gggacgctcg acctgctgtg gcagtgcgcc aatcccgcct ggtcgcgcca tgcggcagcg
     2041 ccgcgcgcgg aagcccgacc gaggcacccg gccgacctgc gcgctctgcg ggagcaactc
     2101 gcacagtcgc gcctcacccg tcgtcacgac tacctgcggc tcgactgggg ctacgagacg
     2161 acgctcgcgc ttcgtgccct gcccgaggag accttcccgg gctggctggg gcagctcacg
     2221 gccagcggtg tcaccttccg attcgccttg cacgtcgagc ccttggctaa agagcgggag
     2281 cgcaggacgc tcacccaggc gctcaagcgc cggcacgcag tgctggccga acgacagggg
     2341 aagggactgc cgcccgacat cgagcaggag gagagctaca gcgaggtcgc cggcgttctg
     2401 cgggacatgg ccgccggcga tctgcggacc ttccgcatcg ccgtcttcgt caccgtccgc
     2461 gccccatccg tcgagcaact gcaggaagcg gttggagcgg tcgtctacgc gctcggcgac
     2521 gccggcggca ccagcgtgga tcgctgcctc ctctggcagc tgccggcctg gcaggcgacg
     2581 ctgccgctgg ctgagaaccc ggctgggatg gagtaccggg tggtgacgcc gaatctggcc
     2641 gacacgctgc ccttcctgcg ccacggcgcg gggacaaagg gcgggccgct ggtcggcttc
     2701 gccgccccct cgcgcgaggc ggtgacactc gacctgctgg accccggtct gcccaatccg
     2761 gcgcttgtcg tctgtggccg ctcaggcagc ggcaagaccc tcttcgcgca ggcctatgcc
     2821 ttcaagcacg tgctgcgcgg tgggcgcgtc gtcgtcctcg accgctcgac tggccattgg
     2881 gacgcgttgg cggcggccat cggcgacgca caagtggtgc cggtcggcct cgacggccgc
     2941 tgccgcctca atccctggca gctacctgat ggcgtccgcg agccggacca ccgcaaactc
     3001 cagtatctcg tggacctaca ccatctcctc ctcggcgagg agggcggcct cgatccggcg
     3061 gcggccgcgc tggtcgaggc cagctgccgt gcggtctacc gcgccaatgc agcgccctac
     3121 gagcgcgacc tggccgcctg gctcgcggct ctggcggccg atccggagca agaggccggg
     3181 cggcgacagc gggcggcgct gctcgcggac cgccttggtc cgtacaccgg cgacggcctc
     3241 tatgcgcgcc tcctcgatgg ggcgacggcg gtccgcgccg acgcgctcct cactatcttc
     3301 aacttcaaag ggctcagcga ccggctggtg ccgctggcga tgctgccgct cctggaatac
     3361 ctctggaccg tcatcgccga cccggcgcgg ccgctcctgc tggtgctgga cgaaggctgg
     3421 aaactgctgg agcacccggc ttcggcgcgc ttcgtcaccg aggtggcgcg caccgggcgg
     3481 catcacggcc tggcgcagct gcacctcagc cagtttccga ccgattacac cggcggggtc
     3541 ggccagacgg tgctggagaa cgcggcggcg acactcctcc tggcgcagca cccgcacctc
     3601 ttagacgact gccagcgcat cttcggcctc acagacgacg agcgggccat cgtcgcccgg
     3661 ctcggcacgg tcaagggcca ggcggccggg gcgtacctgc gcgctggcga aggcgccgac
     3721 agcggcgcgg tggagctgtg ggtcacaccg acagagtact ggctctgcac cagtcaccca
     3781 cccgaggtcg cattgcgcgc ggcggcgctg cgtcgccaca gcggcgacgt ctggacagca
     3841 gtgcgggaac tcgcggcgat gtcgcccgct ggtcgggcga cgctcggcgc gctcgcccag
     3901 ccggtgacgg cggctgcagc gggcggcgtg tgatcgcggg cgggcgcggt ggacgaggac
     3961 ggcagtggcg caggcggagg tctcgcgggc tgtgggtgcg tcgccacca
//
LOCUS       DASUBI010000166         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_11193, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000166 DASUBI010000000
VERSION     DASUBI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11193"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFU72_03225"
     CDS             <1..987
                     /locus_tag="VFU72_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5422528.1"
                     /translation="PALEALRAAYPSAKITLLGAPWHQAFLAGRPGPLDRVLVVPPCF
                     GVRESGPHDPRSDAAELEPFFAAMRAERFDLALQLHGGGANSNPFVRRLGARVTAGFR
                     APGAPPLDRWAPYDYWQREVLRLLEGVALVGARPVTLDPRLALLPGDHEEAARLLGDG
                     AGPLVALHPGSSDVRRRWPTEGFAAVGVTLARHGARLVVTGGADERELAETVAAGIPG
                     ARALVDALSLGGLAGLFARCDLVVANDTGPLHLARAVGAATVGLYWCGNVVNAGPMTQ
                     ARHRTLLSWITTCPVCGRREGEGAPCTCPVSFVAQIAPEAVIEACLGLLGEG"
     gene            complement(1115..1930)
                     /locus_tag="VFU72_03230"
     CDS             complement(1115..1930)
                     /locus_tag="VFU72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422529.1"
                     /translation="MPYADAGAVRLYYESAGEGTPFILQAHHHSAWMPFQLPYFARFY
                     RVITYDRRGTGRSDSPPGPWTAADLARDLRGLMDTLGVERAIVGGASLGGAVACQFGL
                     DYPERARALIVGHTTPWLWPLAVAWLEEQIEAVRRGERPIVLQPRSYEWEAAGPPTAA
                     PGFAETLAGRFLGTLGGGLGQSPEAAIQALEVLLRWDLRPRYAELRELHVPALVIVGG
                     NEPQKTIELAYEWHQQFGVSDFVILPNTHHGAARENPIGWNEAVHGFLRRHGL"
     regulatory      2012..2128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2183..>2451
                     /locus_tag="VFU72_03235"
     CDS             2183..>2451
                     /locus_tag="VFU72_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase"
                     /protein_id="HEU5422530.1"
                     /translation="MNQDVREHELPEIGKISPEIFHEIIKPRLGAGNPNVLVGPQHGI
                     DVGIVDLGAGQVMAISTDPFFVVPEYGWQRAGWFAVNILASDAATS"
BASE COUNT          326 a          895 c          887 g          343 t
ORIGIN      
        1 cccgcgctgg aggcgctgcg cgccgcctac ccctcggcca agatcacgct gctcggcgcg
       61 ccctggcatc aggcgtttct cgccgggcgg cccggcccgc tcgatcgcgt gctggtcgtg
      121 cctccctgct tcggcgtgcg cgagagcggc ccgcacgacc ctcgcagcga cgcagccgag
      181 ctggagccgt tcttcgccgc catgcgcgcc gagcgcttcg acctggcgct ccagctccac
      241 ggcggcggcg ccaactccaa ccccttcgtg cgccggctcg gcgctcgcgt caccgccggc
      301 ttccgcgcgc cgggcgcgcc cccgctcgac cgctgggcgc cctacgacta ctggcagcgc
      361 gaggtgctgc gcctgctcga aggcgtggcg ctggtcggcg cccgcccggt cacgctcgat
      421 cccaggctcg cattgcttcc aggggatcat gaggaggcgg cacggctgct cggcgatggc
      481 gccgggccgc tggtggcgct ccaccccggc agcagcgacg tgcggcggcg ctggccgacg
      541 gagggcttcg ccgcggtcgg cgtgacgctg gcgcggcacg gcgcgcggct ggtggtgacc
      601 ggcggcgcgg acgagcggga gctggcggag acggtcgcgg ccgggatccc tggcgcgcgc
      661 gcgctggtgg acgccctctc gttgggcggt ctggccgggc tgttcgcccg ctgcgacctg
      721 gtcgtcgcca atgacaccgg gccgctgcac ctggcgcggg cggtcggcgc ggccacggtc
      781 ggactctact ggtgtggcaa cgtggtcaac gccgggccga tgacccaggc gcggcaccgc
      841 accctgctct cctggatcac cacctgcccc gtctgtggcc gccgtgaggg cgagggcgcg
      901 ccctgcacct gccccgtctc ctttgtggcc cagatcgccc ccgaggccgt catcgaggcg
      961 tgcctggggt tgctgggaga agggtagccc tcatcccgca cggagacccg cccttctccg
     1021 aacggagcca atgctgggag aaggggagcg tgtgacgaac ggtccttctc cctctcccag
     1081 cattggaaga gggctggggt gagggcccgt cccgctacaa cccgtgccgg cgcaggaagc
     1141 cgtggacggc ctcattccag ccgatggggt tctcgcgcgc cgcgccgtgg tgggtgttcg
     1201 gcaggataac gaagtcggag acgccgaact gctggtgcca ctcgtacgcc agctcgattg
     1261 tcttctgtgg ctcgttgccg cccacgatca ccagcgccgg cacatgcagc tctcgtagct
     1321 ccgcatagcg cggacgcaga tcccagcgca gcagcacctc cagcgcctgg atcgcggcct
     1381 cgggcgactg gcccaggccg ccgccgagcg tacccaggaa gcgcccggcc agggtctcgg
     1441 cgaagcccgg cgcggcggtc ggcggtccgg ccgcctccca ctcgtaggag cgcggctgca
     1501 gcacgatcgg ccgctcgccg cggcgcaccg cctcgatctg ctcctccagc caggctaccg
     1561 ccagcggcca gagccagggg gtggtgtggc cgacgatcag cgcccgcgcg cgctcggggt
     1621 agtcgagccc gaactggcag gcgaccgcgc cgccgagcga ggcgccgccg acgatggcgc
     1681 gctcgacgcc cagggtatcc atcaacccgc gcaggtcgcg cgccagatcg gccgccgtcc
     1741 acggcccggg cgggctgtcc gagcgcccag taccgcgccg gtcgtaggtg atcacgcggt
     1801 agaagcgcgc gaagtagggg agctgaaagg gcatccaggc gctatggtgg tgcgcctgga
     1861 ggatgaacgg tgtcccttcc ccggccgact cgtagtagag ccggaccgca ccggcgtccg
     1921 catagggcat gacccatctc ctctcatcga tcgtcgcgcg cagtgtgccc gctggcgcgg
     1981 cgcttgggaa gagcgcgcgg gcgtgctaga gtacccgtgc tgggggagcg cagaccgcaa
     2041 cgcgggcgtg ctgagagggt ggccgagcca ccgacccttg gaacctgaac cgggtcatgc
     2101 cggcggaggg aagcaccgcc aagagccaga ggacggcccg cgctccagcc cggcgcgggc
     2161 cgcgcgtttg gagggcagcg ggatgaacca ggacgtccgc gagcatgaac tccccgagat
     2221 cggcaagatc tcgcctgaga tcttccacga gatcatcaag ccccggctcg gcgccggcaa
     2281 ccccaatgtg ctggtcggcc cgcagcacgg gatcgatgtc ggcatcgtcg atctcggcgc
     2341 tgggcaggtg atggccatca gcaccgatcc cttcttcgtc gtgccggagt acggctggca
     2401 gcgcgccggc tggttcgcgg tcaacatcct ggcctccgac gccgccacct c
//
LOCUS       DASUBI010000167         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_363238, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000167 DASUBI010000000
VERSION     DASUBI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363238"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFU72_03240"
     CDS             complement(<1..564)
                     /locus_tag="VFU72_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HEU5422531.1"
                     /translation="MGRALRYVGLLVGIVIGWGIGSSWHPAPIDKVPLGFPLFLAALL
                     GALLFLITPYITLGIFHWLHQEIRRLSAIDIIAVAVALVIGGVISALLAWPLSLLPNV
                     FGRLLPALGALAICSVSVVAFLTKKRELQALLIRREPTSEIPDAFAPILLDTSAIIDG
                     RIADLAGSGFLPGDLVVPQFVLRELQLV"
     gene            complement(802..1137)
                     /locus_tag="VFU72_03245"
     CDS             complement(802..1137)
                     /locus_tag="VFU72_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU5422532.1"
                     /translation="MDMVTIDDFQRLDLRTGEITAVEDFPRARKPAYRVTVDFGPEMG
                     PKRSSIQATNYAKDALQGMQVIAVVNLPPRNIAGFISEVLILGVPGADDQIALLTPSR
                     AARLGGAVY"
     gene            complement(1429..1644)
                     /locus_tag="VFU72_03250"
     CDS             complement(1429..1644)
                     /locus_tag="VFU72_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEU5422533.1"
                     /translation="MAEQRRGRVEPGMQVVTSDNKLVGFVKDVDDARMRVEHLPLPDL
                     EIPLDAVYAIAGERVILTMAGDELKPG"
     gene            complement(1716..3440)
                     /locus_tag="VFU72_03255"
     CDS             complement(1716..3440)
                     /locus_tag="VFU72_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5422534.1"
                     /translation="MSAQPEKQRQPRTEWTRGGGGEPEPGRLLAAVIDALPGAAYVLA
                     ADASRTPLAVSEGFAALTGYQPRQWLDDPLFLLRHAHPDDRALLLEVPGEALDRTFRL
                     VAEDGRVVGLYEQARLASGGAGQPQLWYGVLLASSAALSDALGSSVLYDPLTGLPTGP
                     LLLDRLRHGLTRLQRRLGSVALLLVDVEQEILTQAVLATVVERLRGCVRPGDTLGRDG
                     NGRFVLVLEDVAAVEEAEDVAERVLGVLAAALPLAGGGGELRLAARAGIAIGTAPEAL
                     AEHLLRDAMTACDQAKPGRHTVFDPAIYARSLSALARERDLRHALAHDDLLVTYQPLV
                     DLTDQRIVAVTAAPYWEHPEQGLLDAAAFRELAQASGLDLTIGRALLDESCAQLGEWQ
                     SVYPTEPPLRLSVALSAYHLRQPDLALDVERALGQGHLAATSLILEIDESVVAGAADR
                     DLEALRALRGQGVRLALGAFGSGVAALTVLRRLPLDLLKLAPEAAAASGTKPEDDASR
                     RALVAVGHALGLPVVATDVETADDVARARSLGCDLAEGRALAPPNLPDAFARLLRLTG
                     GRLPEIAG"
     gene            3695..4888
                     /locus_tag="VFU72_03260"
     CDS             3695..4888
                     /locus_tag="VFU72_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422535.1"
                     /translation="MLHEPGNRLLAAASAVHLLNDACFAILYPLLPLIAADLNLNYAQ
                     VGLLKATFSGASSLLQIPAGMLGASLGEYLVLLLGNIWVGLGVVAMGLAGSFLGLLAL
                     AALAGLGGNAQHPLANSAVSQRTTPARMATALGTLNFAGDLGKFIGPVIAGIVAVRFG
                     WRYGLGTIGVITALVTLGLLLRRGLVEPPATTEEPAPAEASARPGAQPRATQRGFSLL
                     LLAGSLDNATRGAALTFLPFVLAHKGLAASTVSLAFGLIFAGGAAGKFACGWLTDRFG
                     PLAVIVVTETVTASALLGFLVSPAAAAIPLALGFGFVLNGTSSAFAVAVTGFIPARNR
                     SRGYGVYFTAALLSSALAPLAYGQLGDVAGLSVVFIVMALLTLAVILAALPIRRALAE
                     TLAGE"
     gene            complement(4898..>5236)
                     /locus_tag="VFU72_03265"
     CDS             complement(4898..>5236)
                     /locus_tag="VFU72_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422536.1"
                     /translation="GQFAPVAGVTALAVSENSHWLAFTVYVPGDNQPRFQGLWLLDLS
                     TSRLRQIPTAAGESVTDLWWAAGVLLYRTIGEAGASMAGVYTGTEPFALYQYDPAASA
                     PVERYRASAP"
BASE COUNT          798 a         1887 c         1795 g          756 t
ORIGIN      
        1 caccagctgc agctcgcgca gcacgaactg cggcacgacc agatcgccgg gcaagaaccc
       61 cgagcccgcc aggtcggcga tgcgcccgtc gatgatcgcg ctagtatcga gcaggatcgg
      121 cgcgaaggcg tcggggatct cggaagtcgg ctcgcggcgg atcagcagcg cctgcagctc
      181 ccgcttcttg gtcagaaagg cgacgaccga gacgctgcag atggccagcg cgcccagcgc
      241 cggcaagagc cgcccgaaga cattcggcag cagcgacagc ggccaggcca gcagcgccga
      301 gatgacccca ccgatgacca gcgccaccgc cacggcgatg atgtcgatgg ccgagaggcg
      361 ccggatctcc tggtgcagcc agtggaagat gccgagggtg atgtacgggg tgatcaagaa
      421 gaggagggcg cccagcagcg cggcgaggaa gagcggaaac ccgagcggca ccttgtcgat
      481 gggcgccgga tgccagctgg agccgatgcc ccagccgatc acgatgccga ccaacagtcc
      541 gacatagcgc agggcccgtc ccaatggagt gcgcgtgcgt tgggcccgag aacgccggtc
      601 agacatcgct tacaccttca gtcgcggttc ctgacccgcc acgtggacgt tcgtaagcaa
      661 ggggagagtg tgccgccgcc atggtggttt ccaggcaggc gagatacccg gaaacgcttg
      721 cgcccgtcca gcgagtgtag catccgcgtt ccagccggtc aacgcgcgcg catgaggcat
      781 gaggaggcgc gggcatcggt ctcagtacac cgcgccgccc aatcgcgccg cgcggctagg
      841 cgtgaggagg gcgatctgat cgtcggcgcc gggcacgccg aggatcagca cttccgagat
      901 gaagccggcg atgttgcgcg gcggcaggtt gaccacggcg atgacctgca tgccttgcag
      961 cgcgtctttg gcgtagttgg tggcctgaat gctggagcgc ttgggcccca tttcagggcc
     1021 gaagtcgacc gtgacgcggt aggcgggttt gcgcgcccgg gggaaatcct cgacggccgt
     1081 gatctctcca gtacgcaggt caagccgctg gaagtcgtcg atcgtcacca tatccatgct
     1141 gccgcccctc tcaactcagc gccgcttccg ccggcgtcga tgatacaggg ccgcggccgg
     1201 atccctccct gggccggagc agcgcggaag cgcctcggtg gccaccgaat gggttggctg
     1261 cagggcaagg acggatcctt cgaccgcgct caggctgaca gggtcagtta ggggctgacg
     1321 aggaggactc tcctcgtccg acaggccgca tcccctaacc ctcgcaacct gagcgcggtc
     1381 gagcgatcga tgtcccacgc ggcacccaat gtgttcgtca gagacaccct agcccggctt
     1441 gagctcgtcg ccggccatgg tgaggatgac gcgctcgccg gcgatggcgt agaccgcgtc
     1501 gagcggaatc tccaggtcgg gcaagggcag gtgctccacc cgcatccgcg cgtcgtccac
     1561 atctttgacg aacccgacca gcttgttgtc cgaggtgaca acctgcatgc ctggctcaac
     1621 ccgcccgcgc cgttgctccg ccactgtcgc cgctcccttc gcatctggcc gcccgccgtc
     1681 gcaccgggag accgcacaat tgctgccggg tggtctcagc cggcgatctc gggcaagcgg
     1741 ccgccggtca gtcgcaggag gcgcgcgaag gcatcgggca ggttcggcgg ggccagcgcg
     1801 cggccctcgg ccagatcgca accaagcgat cgcgcccgcg cgacatcgtc cgccgtctcc
     1861 acgtccgtcg ccaccaccgg cagaccgagc gcgtggccta ccgccaccag tgcgcgccgg
     1921 gatgcgtcat cctccggctt cgtaccggat gcggcggccg cctctggagc gagtttgagc
     1981 aggtcgagcg gcaggcgacg cagtaccgtc aatgccgcca cgccggagcc gaacgcgccg
     2041 agcgccagcc ggacgccctg accacgcagc gcgcgtagcg cctccagatc gcgatccgcc
     2101 gcgccagcca cgaccgattc atcgatctcc agtatcagcg aggtcgccgc gaggtggcct
     2161 tgccccagcg cacgctcgac gtcgagcgcg agatccggct gccgcagatg gtacgccgag
     2221 agcgcgacgc tcagccggag cggcggctcg gttgggtaga cactctgcca ctcgccgagc
     2281 tgggcgcagg actcgtcgag gagcgcgcgg ccgatggtga ggtccaaccc gctcgcctgc
     2341 gccagctcgc gaaacgccgc cgcatcgagc aggccctgct cggggtgctc ccagtagggc
     2401 gccgccgtga cggccacgat gcgctggtcg gtgaggtcga ccagcggttg ataggtcacg
     2461 agcagatcgt cgtgggccag cgcgtggcgc aggtcacgct cgcgtgccag cgccgacaac
     2521 gagcgcgcgt agatggccgg gtcgaagacg gtgtgccggc cgggcttggc ctgatcacac
     2581 gccgtcatcg cgtcgcgcag gaggtgctcg gccaacgcct cgggcgcggt gccgatcgcg
     2641 ataccggccc gcgccgccag gcgcagttca ccgcccccgc ccgccaacgg cagcgccgcc
     2701 gccagcacgc cgagcacgcg ctccgcgacg tcttccgcct cctcgaccgc ggccacgtcc
     2761 tccaggacga gcacgaagcg gccgttgcca tcgcgcccca gcgtgtcgcc cgggcgcaca
     2821 cagccacgga ggcgctcgac caccgtcgcc aggacggctt gggtcagaat ctcctgctct
     2881 acatcgacca gcagcaacgc gaccgagcca agccgccgct gcagccgggt cagcccatgg
     2941 cgcaggcgat cgagcagcaa ggggccagtc ggcaaaccgg tgaggggatc atagagcacg
     3001 ctggatccga gggcgtcgct gagtgcggcg ctgctcgcca acagcacgcc gtaccagagc
     3061 tggggctgtc cggcgccccc cgacgccagc cgcgcctgct cgtacagccc gacgacgcgg
     3121 ccatcctcag ccaccaggcg gaaggtgcgg tcgagcgcct cgccgggcac ctcgagcagc
     3181 agggcgcggt catccgggtg ggcgtggcgc agaaggaaca gcggatcgtc aagccactga
     3241 cggggctgat agccagtcag ggccgcgaag ccctcgctca ccgccagcgg agtgcgcgac
     3301 gcgtcggcgg ccaggacgta ggccgcaccc ggcagcgcgt cgatcaccgc ggcgagcagc
     3361 cgccccggct ctggctcccc tccaccgccg cgggtccact cggtccgcgg ctgccgctgc
     3421 ttctctggtt gcgcactcat ccggctgctc tcccatctca gccgttcgcc catggcgcta
     3481 gcgtacaaac gccgccgccg ccgtgccata cccattttgt atgggattct acggtacggc
     3541 gtcccaaacc agccgattcc gtcctgccgc acggaccagg tagtatcatg acagccgcgt
     3601 atcacgatga cgcggtccag atcgtgccgc gatgcgggcg ggagccggcg tgtcgacctc
     3661 tttgcaacgc ctccgcagcg tggcgtcaac cgccatgctc cacgaaccgg gcaaccggct
     3721 gctggcggcc gcttcggcgg tccacctgct gaacgacgcc tgcttcgcca ttctctatcc
     3781 cttgctcccc ctgatcgcgg ccgatctcaa cctcaactac gcccaggtcg gcctgctgaa
     3841 ggccaccttc tccggcgctt cgagcctgct gcagattcca gccgggatgc tcggcgcgtc
     3901 gctcggcgag tacctggtgc tcctgctcgg caacatctgg gttgggctcg gcgtggtggc
     3961 gatggggctg gccgggagtt tcctggggct gctggcgctg gcggccctgg cagggttggg
     4021 cggcaatgcc cagcatcctc tagcgaactc ggccgtctcc cagcgcacca cgccggcgcg
     4081 catggcgacc gcgctcggca cgctcaactt cgccggcgat ctcggcaagt tcatcggccc
     4141 ggtgatcgcc ggcatagtcg cggtacgctt cggctggcgc tacggcctcg ggacgatcgg
     4201 cgtcatcacc gccctggtca ccctcggcct gctgctgcgc cggggcctgg tcgagccgcc
     4261 cgcgacgacc gaggagccgg cgccggcgga ggcaagcgcg cgccccggcg cgcaaccacg
     4321 agcgacgcag cgcggcttct cgctgctgct gctggccggc agcctggaca acgccacccg
     4381 cggcgcagcg ctcaccttcc tgcccttcgt actggcgcac aaggggctgg cggccagcac
     4441 cgtcagcctc gccttcgggc tgatcttcgc gggcggggcc gcgggcaagt tcgcctgcgg
     4501 ctggctgacc gaccgcttcg gcccgctggc ggtcatcgtc gtgaccgaga cggtgactgc
     4561 ctcggcgttg ctcggcttcc tggtcagccc ggccgccgcc gcgatcccgc tggcgctcgg
     4621 cttcggcttc gtgctcaacg gcacctcgtc cgccttcgcc gtggcggtga cgggcttcat
     4681 cccggcgcga aatcgctcgc gcggctacgg cgtctacttc accgccgcgc tgctctcatc
     4741 ggcgctggcg ccgctcgcct acggccagct cggcgacgtc gccggcctga gcgtcgtctt
     4801 catcgtgatg gcgctgctga cgctcgccgt catcctcgcc gccctgccga tccggcgggc
     4861 gctggccgag acgctggcag gagagtagcc cggcgggcta gggcgccgaa gcgcggtagc
     4921 gctcgaccgg agcgctggcg gcggggtcgt actggtagag cgcgaagggc tcggtgccgg
     4981 tgtagacccc ggccatgctc gcacccgctt cgccgatcgt gcggtagagc agcacgccgg
     5041 ccgcccacca gaggtcggtg accgactcgc ccgccgcggt ggggatctgt cgcagccggc
     5101 tggtgctcag gtcgagcagc cagagcccct ggaagcgcgg ctggttgtca ccgggaacgt
     5161 agacggtaaa ggccagccag tggctattct ccgagacggc cagcgccgtc acgccggcta
     5221 cgggcgcgaa ctgccc
//
LOCUS       DASUBI010000168         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_526693, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000168 DASUBI010000000
VERSION     DASUBI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526693"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1260)
                     /locus_tag="VFU72_03270"
     CDS             complement(58..1260)
                     /locus_tag="VFU72_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEU5422537.1"
                     /translation="MYLGLLRKAGVEPDADFLREAVRVLGQALMEAEVAAHLGAERHE
                     RTPERSGQRNGYRERQWDTRVGTIDLKVPRVRDGSYFPTLLEPRKRAERALAAVVQEA
                     YVQGVSTRRVDELVRALGMSGISKSQVSRVCQELDAEVERFRTRRLDDGYPYVWLDAT
                     YVKARQDGRVVSLAVVIAIGVRTTGEREVLGLDVGASEDGAFWLQFLRALVARGLRGV
                     QLVISDAHEGLKGAIAAALHGAGWQRCRVHFVRNALALVPKAAQQLVAATIRTVFAQP
                     DATGTREQWRRVADNLRPRFPKLGDLLDAAEADVLAYLAFPAAHWRQIWSNNPLERLN
                     REIKRRTDVVGIFPDAAAVIRLVGAVLAEQHDEWQVSRRYFSAESLALLDAATIPAHA
                     GPALLAAD"
     gene            1312..1878
                     /locus_tag="VFU72_03275"
     CDS             1312..1878
                     /locus_tag="VFU72_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422538.1"
                     /translation="MRKPLEIPELTAAEAEALDTLYRTTRDVRLRTRAQIVLLAGEQR
                     LTAPAIARIVRESDQTVRTWLKRWLAEGVEGLQDRPMPGGPTKITAAYREQLLAAVRQ
                     RPRCLGRPYSLWTLQRLADYLAELTGIRLTYETVRVALKAGGIVLSRPQHTVSSPDPE
                     YHVKKRRSSMRATTSPPATPSTMRTSST"
     gene            1875..2372
                     /locus_tag="VFU72_03280"
     CDS             1875..2372
                     /locus_tag="VFU72_03280"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU5422539.1"
                     /translation="MSWLPTLRAMWSPKGQQVMIPTPGQPDRYYGIGAVNYHTGETVV
                     HFEHRKRRKEIAALLKALLAKHPTGTVYVAWDNVGTHEDDEVEAVVRAAAGRLVLLYL
                     PTYSPWLNPIEMLWRHFRREVTHCECFPTKQALLAAARDFFAHHNLQPQKILSVIGSK
                     ATNLI"
     gene            complement(2390..>3111)
                     /locus_tag="VFU72_03285"
     CDS             complement(2390..>3111)
                     /locus_tag="VFU72_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422540.1"
                     /translation="ASDRRNGARHPRPAGHQVAACQALARQPRPGVRAKKSQRDRLSR
                     LAETHPDWALGFADETWWSRLAVPGLHAWAEPQRPLRLVEQAVATDDPDPKALAAYGL
                     YLPADNEVLLRFVDGRPVSALTTQFLAWCCANLAARGTTALLLVWDNAGWHISKEVRA
                     WVRGHNRAVKRGEAAVRLVVCQLPVQSPWLNPIEPKWVHGKRRAAEPNRLLTGRETAE
                     RACAALGCAYEKHLALPQKVA"
BASE COUNT          477 a         1136 c         1032 g          466 t
ORIGIN      
        1 gcccttctca gcctggcgct gggtgcgatt cccgggaatc gcaccctgcg ccagggatca
       61 atcggcggcg agcagcgctg gccccgcgtg tgccgggatg gtggccgcgt ccaggagggc
      121 gagcgactcg gcgctgaagt agcgcctgct cacctgccac tcgtcgtgct gctcggccag
      181 cacggccccg accaggcgga tgacggccgc cgcatccggg aagatgccga ccacgtcggt
      241 gcggcgcttg atctcgcgat tgagccgctc cagcgggttg ttcgaccaga tttgccgcca
      301 gtgggcagcg gggaaggcaa ggtaggccag cacatctgcc tcggccgcgt ccagaagatc
      361 gcccagcttc gggaagcggg gccgcagatt gtcggcgacc cgccgccact gctcgcgggt
      421 gccggtcgcg tcgggctgcg cgaagacggt gcggatcgtc gcggcgacga gttgctgcgc
      481 cgccttcggc accagcgcga gggcgttgcg cacgaaatgg acgcggcagc gctgccagcc
      541 cgccccgtgg agcgccgccg cgatggcccc cttgagtccc tcgtgcgcgt cgctgatgac
      601 cagctgcacg ccccgcaacc cgcgcgcgac cagggcgcgt aggaactgca gccagaacgc
      661 accatcctcg ctcgcgccca cgtcgagccc cagcacctcc cgctcgcccg tcgtccgcac
      721 gccgatggcg atgacgaccg ccagagagac gacccgcccg tcctgccgcg ccttcacgta
      781 ggtcgcgtcc agccagacgt acgggtagcc gtcgtccagg cggcgcgtgc ggaagcgctc
      841 gacctcggcg tccaactcct gacacacccg cgagacctgg ctcttgctaa tgccgctcat
      901 ccccagcgcc cgcaccaact cgtccacccg ccgcgtcgag accccctgca cgtaggcctc
      961 ctgcacgacc gccgccaatg cccgctccgc ccgcttgcgc ggctccagca gcgtcgggaa
     1021 gtagctcccg tcgcgcaccc gcggcacctt caggtcgatc gtgccgacgc gcgtatccca
     1081 ctggcgttcg cggtagccgt tccgctggcc actgcgctct ggggtccgct cgtggcgctc
     1141 cgccccgaga tgcgccgcca cctccgcctc catcagcgct tgccccagca cccggaccgc
     1201 ctcgcgcaag aagtccgcgt ccggctctac ccccgccttg cggagcaacc ctaagtacaa
     1261 tcagatgaga ttcgtgggaa tgcggtatgc tggcggcggg aggaggccgc catgcgtaag
     1321 ccgctggaaa ttcctgagtt gaccgccgcc gaggcggagg ccctggacac actgtaccgg
     1381 acgacgcgcg atgttcgcct gcggacccgc gcgcagatcg tgctgctggc gggcgagcag
     1441 cgcctcaccg cccccgccat cgcgcggatc gtgcgcgagt ccgatcagac agtccgcact
     1501 tggctcaaac gctggttggc cgagggggtg gaggggctgc aggatcgccc gatgccaggc
     1561 gggccgacaa agatcaccgc tgcctaccgg gagcagctgc tcgccgccgt gcggcaacgg
     1621 ccgcgctgcc tggggcggcc ctactccttg tggacgttgc agcgtctcgc ggactacctg
     1681 gccgaactga ccggcatccg gctgacctac gagacggtgc gtgtcgcgct caaggcgggg
     1741 ggcatcgttc tgagccgccc gcagcatacc gttagcagcc ccgaccccga gtaccacgta
     1801 aaaaaacggc gatcgagcat gcgcgcgacc acctccccac cggcgacgcc ttctactatg
     1861 cggacgagtt caacctgagc tggctcccca ccctgcgcgc gatgtggagt ccgaaggggc
     1921 agcaggtgat gatcccgaca cccgggcagc cggaccgcta ctatggcatc ggcgcggtga
     1981 actaccatac cggcgagacg gtcgtgcatt tcgagcaccg caagcgccgc aaagagatcg
     2041 ccgcgctcct gaaggcgctg ctggcgaagc acccgactgg caccgtttac gtggcctggg
     2101 ataatgtggg cacgcacgag gacgacgagg tcgaggcggt cgtgcgcgcc gctgccgggc
     2161 gcctggtcct cctctatctg ccgacgtaca gcccgtggct caacccgatc gagatgctct
     2221 ggcgccactt ccggcgcgag gtgacccact gcgagtgctt cccgaccaag caggccctgc
     2281 tcgccgccgc ccgggacttc ttcgcccacc acaacctgca gccgcagaaa atactgtccg
     2341 tgatcggctc gaaagccacg aatctcatct gattgtactt agtgcatcat caggccacct
     2401 tctgtgggag ggctaagtgt ttctcgtagg cacagcccaa cgccgcgcag gcgcgctcgg
     2461 cggtctcgcg cccggtcagc aggcggttgg gttcggccgc ccggcgcttg ccgtggaccc
     2521 acttcggctc gatggggttg agccacggac tctgcaccgg caactggcag accaccagcc
     2581 gcaccgctgc ctcgccgcgt ttgactgccc ggttgtgccc cctgacccaa gcgcgcacct
     2641 ccttactgat gtgccagccg gcgttatccc acacgagcag cagggctgtc gtgccgcggg
     2701 ccgcgaggtt ggcacagcac caggccagaa actgggtcgt cagcgcgctg accggccggc
     2761 catcgacgaa gcgcagcagg acctcgttgt cggccggcaa gtagaggccg taggccgcga
     2821 gggccttggg gtcggggtcg tccgtggcga ccgcctgctc gaccaggcgc aggggccgct
     2881 gcggctcggc ccaggcgtgc agcccaggta ccgcgagacg gctccaccag gtctcgtccg
     2941 cgaagccgag cgcccagtcg ggatgggtct cagccaggcg gctcaggcgg tcgcgctggc
     3001 tttttttcgc gcgtacgccg ggtcggggct gacgagccag cgcttggcac gctgccactt
     3061 gatgcccagc cgggcgaggg tggcgcgcac cgtttcgccg gtcactggcc c
//
LOCUS       DASUBI010000169         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_63375, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000169 DASUBI010000000
VERSION     DASUBI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63375"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="VFU72_03290"
     CDS             complement(<1..1972)
                     /locus_tag="VFU72_03290"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU5422541.1"
                     /translation="MSKHREKTFEDEVVAHLTANGWRLGNPARYDRARALYPDDVLGW
                     LRDTQPQALAKVAAPAGQTVDDVVLDRLAAVLDKDGPLRVLRRGFKHINAQFAMCQFK
                     PSQGLNPDTLARYAQVRCTVIRQVRYSRHKGDSIDLVFFVNGLPVATAELKTDFTQRI
                     HDAIKQYRQDRPPRDPAANQAEPLLTFKRGALVHFAVSTDEVAMTTRLAGPDTHFLPF
                     NLGHDGGAGNPPNPDGYRTAYLWERVLQRDSLLDILGNFLHLERTEEATPDGGTRVRE
                     ALIFPRYHQWEAVGRLLAAARDEGPGHNYLIQHSAGSGKSNSIAWLAHRLATLHDDGD
                     RKVFDSVIVVTDRTVLDRQLQETIYQFEHKSGVVVPIKDDGPSKSEQLKTALLDRAPI
                     IIVTLQTFPFVVEAIRQQASLRDRAFAVIADEAHSSQTGSAASNLARVLTAEQLAEGE
                     EVAAEDVLLAAMEARAAHRNISYFAFTATPKAKTLQRFGRRPNPALPESDTNLPEAFH
                     VYTMQQAIEEGFILDVLQNYTTYRLAFRLAHNGAEYDEAEVDRARALKSLMRWVRLHP
                     YNIAQKVEVIVEHFHHNVLTLLEGRAKAMVVTGSRKEAVRYKLALDAYVKRQGYRDVA
                     ALVAFSGEVGDPESGPDPFSEHSMNPGLKGRDI"
BASE COUNT          272 a          682 c          769 g          363 t
ORIGIN      
        1 ggatgtcgcg ccctttcagg ccggggttca tcgagtgctc gctgaacggg tcggggccgc
       61 tctccgggtc gccgacctcg ccggagaacg cgaccagcgc cgcgacgtcg cggtagccct
      121 gccgcttcac gtaggcatcc agcgccagtt tgtagcgcac cgcctccttg cgcgagccgg
      181 tgacgaccat cgccttcgcc cgcccctcca gcagcgtcag gacattgtgg tggaagtgct
      241 cgacgatgac ttcgaccttc tgcgcgatgt tgtaggggtg cagccgcacc cagcgcatga
      301 ggcttttcag cgcccgcgcc cggtccacct cggcctcgtc gtattccgcg ccgttgtgcg
      361 ccagccggaa ggcgaggcgg taggtggtgt aattttgcag cacgtcgaga atgaagcctt
      421 cctcgatcgc ctgctgcatc gtgtagacgt ggaacgcctc gggcagattc gtgtccgact
      481 ccggcagggc cgggttgggg cggcggccga agcgttgcag cgttttggcc ttcggggtgg
      541 cggtgaaggc gaagtaactg atgttgcgat gcgccgcccg cgcctccatc gccgccagca
      601 gcacgtcctc ggcggcgacc tcctcgccct ccgccagttg ctcggcggtc agcacgcggg
      661 ccagattgct ggcggcggag ccggtctgcg aggagtgcgc ctcgtcggcg atcacggcga
      721 aggcgcggtc gcgcaggctc gcctgctggc ggatggcctc cacgacgaac gggaaggttt
      781 gcagcgtcac gatgatgatc ggcgcgcgat cgagcagcgc cgttttgagt tgctccgact
      841 tggacgggcc gtcgtccttg atcggcacga ccacgccgct cttgtgctcg aactggtaga
      901 tcgtctcctg caactgccgg tcgagcaccg tgcggtcggt gacgacgatc accgagtcga
      961 agaccttgcg gtcgccgtcg tcgtgcaggg tggcgagccg gtgcgccagc caggcgatcg
     1021 agttggattt gcccgagccg gccgagtgct ggatgaggta gttgtggccg gggccttcgt
     1081 cgcgcgccgc cgcgagcagg cggcccaccg cctcccactg gtggtagcgc gggaagatca
     1141 gcgcctcgcg gacgcgcgtg ccgccgtccg gcgtcgcctc ctccgtgcgt tccaggtgga
     1201 ggaagttgcc gaggatgtcg agcaggctat cccgctgcaa cacgcgctcc cacaggtagg
     1261 cggtgcggta gccgtccggg ttgggcgggt tgcctgcgcc gccgtcgtgc cccaggttga
     1321 agggcaggaa gtgggtgtcc ggcccggcga gccgcgtcgt catcgccacc tcgtcggtgc
     1381 tgaccgcgaa gtggaccagc gcgccccgct tgaaggtcag cagcggctcg gcctggttgg
     1441 cggccgggtc gcgcggcggg cggtcctgcc ggtactgctt gatggcgtcg tggatgcgct
     1501 gcgtgaagtc ggtcttcagt tcggcggtgg cgaccggcag gccgttgacg aagaagacga
     1561 ggtcaatgct gtcgcccttg tgccggctgt agcgcacttg gcggatgacg gtgcagcgga
     1621 cctgcgcgta ccgcgccagc gtgtcggggt tgaggccctg gctcggcttg aactggcaca
     1681 tggcgaactg ggcgttgatg tgcttgaagc cgcgccgcag gacgcgcagc ggcccgtcct
     1741 tatcgagcac ggcggccagc cggtcgagca ccacgtcgtc caccgtctgc cccgctggcg
     1801 cggcgacctt cgccagcgcc tggggctggg tatcgcgcag ccagccgagc acgtcgtcgg
     1861 ggtagagggc gcgcgcccgg tcgtagcgcg cggggttgcc gagccgccag ccgttggccg
     1921 tgaggtgcgc gacgacctcg tcctcaaacg ttttctcgcg gtgcttgctc atggcgcgac
     1981 cccggcggcg agcggcgcgg cgctctcagc ggtgacggcg tcgcgcacgt caatcttgcc
     2041 ggtgacggca gcggcaatca gcgccgtgcg gtgctcgcgc agcagg
//
LOCUS       DASUBI010000170         9005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_759216, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000170 DASUBI010000000
VERSION     DASUBI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759216"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..739
                     /locus_tag="VFU72_03295"
     CDS             323..739
                     /locus_tag="VFU72_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5422542.1"
                     /translation="MSRTPIRSLGEIALQVGDMDRMVDFYTNVVGLELLRRFPGNGPA
                     FFRIAPGVEGHTQVLALFDRGERGESRARRPPLDHIAFSLSLADLAAERERVRALGVP
                     LREETHAWVQWRSFYIDDPEGNVVEWVAYDPAIPRE"
     gene            872..1657
                     /locus_tag="VFU72_03300"
     CDS             872..1657
                     /locus_tag="VFU72_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5422543.1"
                     /translation="MQEYQSLFRLDGKRALVVGAGSGIGQASALGLAAFGAEVVCADI
                     NTGPVAALARQINDEGGKARALTVDLLDPASVQAAAGAVGTPDVLVSTPSINVRKPIL
                     QLSEEEFDRIVGLNLKGTFRLVKAFAPGMAERGSGSIIAFSSIRAQVVEPGQGVYAAT
                     KAGTVQMFRALAVELGERNVRVNVIAPGVVETPLTAQIKSHPEWYRAYADKTMLRRWA
                     QPSEMVGAVVYLASDASSYVTGSYLLVDAGWLAADGRFTPPLS"
     gene            1668..2900
                     /locus_tag="VFU72_03305"
     CDS             1668..2900
                     /locus_tag="VFU72_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5422544.1"
                     /translation="MTAHEHVSLTQTLARIDRGELVRLTQDLVRIPSVVRPDDGGNEQ
                     TAAEFVAGFLERHGLPVFRDEAAPGRPNVWAVLEGTQQGKTLLFEAHTDVVTEGDPAS
                     WSQPPFAGAVVGSRIYGRGACDTKGNLAAAVLAVLAIKRAGLDFPGRIVLCHPVDEEG
                     LMLGIKRFIAAGHAEGVDAAIICEPEENQLCIAQKGALRVAVRFSGRMAHGAMPLSGV
                     NPITRAARFVVAVEALERDEIERHGRDPLLGYPSLTPTVLRAPDGGEPQLNVIPAAAS
                     VALDIRTIPSQSHQGLLARLRECLDWLRADDPLLRAELTVLDDRPCTETPRDSAIVRA
                     ASDAYRTITGSEPRFNGVPGATDGTFLHAWAGIPIVTTGAGQRTVPHHADEWVDIDEL
                     ETTCRLYAATAMRYLGAD"
     gene            complement(3112..3267)
                     /locus_tag="VFU72_03310"
     CDS             complement(3112..3267)
                     /locus_tag="VFU72_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEU5422545.1"
                     /translation="MTDNLGYLFAAFLISWVVIAGYIWSLGRQVHSLRQEVQALTEED
                     AGVPRVG"
     gene            complement(3264..4037)
                     /gene="ccsA"
                     /locus_tag="VFU72_03315"
     CDS             complement(3264..4037)
                     /gene="ccsA"
                     /locus_tag="VFU72_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5422546.1"
                     /translation="MKSEGGAPGWGPILGGLTGLTMALSLYLMFIYAPTDAVQGNAQR
                     IFYIHVPMAWDAYLAFFVVFLAGIGYLWKRSVFWDQLGRASAEIGLVLTTLVLITGSL
                     WGRPIWGTWWTWDARLTTTLILWFMYLGYFMLRAYAGERERAARFAAVLGIVAFIDVP
                     INYLSVQWWRTLHPTQVVSLEHAAMPGSMLLALMVSLVAFTLLYAFLLQQKWAIEQAK
                     DRLIDYQLAEYERQSQADTPATSQPQSQQPAVQEGVSLG"
     gene            complement(4044..4712)
                     /locus_tag="VFU72_03320"
     CDS             complement(4044..4712)
                     /locus_tag="VFU72_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5422547.1"
                     /translation="MNALGKIGTLVWKDLLTELRGKELFLGMFVFAVLTIVTFNFAFD
                     LTGVAKAPSGAGALWVAFSFAGMLGLGRAVALERDRGSFDGLLLCPVDRGVIYLGKLV
                     GNLIFIGLVEIVAVPVFGALFDLPVLRPLLFLVLALGTIGFAAVGTLLAVMAASTRAR
                     EVMLPVLLFPLALPVVIATVRATTLVLTNQGDGLLDWINLLIGFDVLFVAIAFLVFDR
                     VVEE"
     gene            complement(4709..5470)
                     /gene="ccmA"
                     /locus_tag="VFU72_03325"
     CDS             complement(4709..5470)
                     /gene="ccmA"
                     /locus_tag="VFU72_03325"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU5422548.1"
                     /translation="MNELQSIPIAQEAPAGTSRAPAPRLQAERVSKRFDNRMVLRPLD
                     LTLAPGERVALFGANGAGKTTLLRILATLARPTTGRILIETVPVERDARRARRALGVV
                     AHQPYLYDDLTAEENLRFFARMYDVPEAERRIEQVLGLVGLRARSGDRVHTFSRGMQQ
                     RLAMGRAILHQPAVLLFDEPDTGLDREGMRLLQRLIGEHAAAGGSVLLTTHDLRFGLG
                     CADRVLLLAQGRLTLDAPVDAVDERQLDQRLAVPS"
     gene            complement(5467..5832)
                     /locus_tag="VFU72_03330"
     CDS             complement(5467..5832)
                     /locus_tag="VFU72_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422549.1"
                     /translation="MLGYLALAAVSIAVFIFVLEPLLGGEQEAAVALPAQLADLRARR
                     QYLIEAIRDVDFDHASGKVGDEEYRETRGRFLREAALATRDLNLETGNLDDELEVEIL
                     KLRALVAREGQPGQAQGDP"
     gene            complement(5832..6299)
                     /locus_tag="VFU72_03335"
     CDS             complement(5832..6299)
                     /locus_tag="VFU72_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEU5422550.1"
                     /translation="MKRSLSTAFAVAVLLLLLPLAAAADEPLSPEALKIANGLNCPVC
                     SGESVRDSQASIAVEMRQVIQQQLDQGQSPDQIKEYFVERYGVSILREPPKQGFYHTL
                     WWMPVAGLAFGAIILALYIMSRPRSLPEPSVDDDELRRLEEQVGQELLADQGM"
     gene            complement(6289..8283)
                     /locus_tag="VFU72_03340"
     CDS             complement(6289..8283)
                     /locus_tag="VFU72_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU5422551.1"
                     /translation="MAQLGSGALIVAFAITLYGIVVSVLGARRQSSELIQSGIRAVWG
                     VTALIVVAVGALITAFLTHDFQLVYVAGRSSREMPIHYVLAAFYGGQEGSLLYWSMIA
                     GLLSSLAIFLHRKRDRALIPYVAATVLTTELFFLVMLNFVASPFTLSPTTPSDGLGLN
                     PLLRDPGMMMHPPFLLGGFASFTVPFGFAMAAMISGRLGDDWIRAIRRWALVSFGILG
                     TGLVLGAWWAYHVLGWGGYWGWDPVENVALIPWLTVTAFIHSIIVQERRGMLKVWNMS
                     LIVASYILAVFATFTVRSGLISSVHTFATSPIGGWFLGYLAIVIVVATGFLAYRFRGL
                     KADRAIEAVVSRESGFLLNNLLFTALAFATFWGTVFPLISELFRNTRLTVGPPFYNQV
                     DGPLMLGVLILMGIGPMLAWRRSPGRLLLRNLLWPILLGVASVPVFQAILGNPLASLG
                     LGAAVFTVTSVVVEYARGARVRRRTMHENYLHALAMLVRRNNRRYGGYIVHLAIAIMA
                     VGIIGSHFFQSERQFTLKPGQTGQVAGFTLTYAALHDTTTNEGEVVSAVVDVSKGGSH
                     QATVETKRFFYTGWESQPTARMGIATVGLNDVYVVLDRWDTDSTASLRVYINPLVSWI
                     WWGAIVYVLGTVTLLWPVARPVARRATRRVREREAVPVEA"
     gene            complement(8296..8751)
                     /locus_tag="VFU72_03345"
     CDS             complement(8296..8751)
                     /locus_tag="VFU72_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU5422552.1"
                     /translation="MEAGAPMAQPVAAAAERRFSWVNPKLLIAGIAILLAVGYLIFSA
                     MQGSASSYFVTVGELQQQSVQMDGKRVRVGGDVVPGTIDKGNAGDPIRFVVGDGTHSI
                     PVVYNGVLPDIFTDNTQVVIEGTYHTNGTFEASTLLTKCPSRFTAKPSQ"
     gene            complement(8780..>9005)
                     /locus_tag="VFU72_03350"
     CDS             complement(8780..>9005)
                     /locus_tag="VFU72_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein CopD"
                     /protein_id="HEU5422553.1"
                     /translation="LVYVIVLGVKIVAALTAFWLTGVFTRRVAGRRQPARSGRAARVN
                     RSLLVLMLGGTAFVLGIALAASYPTGIGQR"
BASE COUNT         1559 a         3117 c         3033 g         1296 t
ORIGIN      
        1 acgtcctcgc cctcgaccac gccgtagcgc ggcccctgct gcgtggcaaa gcgcacgatc
       61 ttcatccggc actcctttcg cccagatggc ccttctacct gggacacgca tcgctgttca
      121 ctcaatccca taatcaggtg cccaactctg ctcgacagac aatagacgcg ggcgcggacc
      181 gggtcaatct gaggcacgga ggtgaagcgc gcgccgcatg aggttgtcac gcggccggcg
      241 tgcgatcatg gggcgtgcgc gccccagcgg catgtcgctg ctggacttca actgcgttca
      301 gaatgatgga aagggaggcc gggtgagccg aacaccgatt cggagtctgg gcgagatcgc
      361 gcttcaggtc ggggacatgg atcgcatggt cgatttctac accaacgtcg tcggcctcga
      421 attgctgcgg cgcttccctg ggaatggccc agccttcttc cgcatcgccc cgggcgttga
      481 gggccacacc caggtgctgg cgctcttcga ccgtggcgaa cgcggggaat cgcgagccag
      541 gcgtccgccg ctcgatcaca tcgcgttcag tctcagcttg gcggacctgg cggccgagcg
      601 tgagcgcgtg cgcgcgctgg gtgtgccgct gcgcgaggag acacacgcgt gggtgcagtg
      661 gcgctcgttc tacatcgacg atccggaggg aaacgtggtg gaatgggtcg cctacgatcc
      721 cgcgatcccg cgtgaataac gctggtggcg cgcagccccg gcccgggcgc tcgagagccg
      781 tctttcgcac tccaatccgg accggtatga tcgacgcggc cacggaaacc cggtcacact
      841 gatgtcacgc ggcagcgaga ggagcaacgg cgtgcaggag taccagtccc tcttccggct
      901 ggacggcaaa cgcgctctgg tggtgggcgc cggcagcggc atcgggcagg ccagcgccct
      961 cgggctggcc gcgttcggcg ccgaggtcgt ctgcgccgac atcaacaccg ggccggtcgc
     1021 ggcgctggcg cgccagatca atgacgaggg cggcaaagcc cgcgcgctga cggtcgacct
     1081 gctcgacccg gccagcgtcc aggcggccgc gggcgcggtc ggcacgcccg acgtgctggt
     1141 ctcgacgccg agcatcaatg tgcgcaagcc gatcctgcag ctgagcgagg aggagttcga
     1201 ccgaatcgtt gggctgaacc tgaaaggcac cttccggctg gtcaaggcgt tcgcgccggg
     1261 catggccgag cgcggctccg gcagcatcat tgccttctcc agcatccggg cgcaggtggt
     1321 cgagccgggc cagggcgtct acgccgccac caaggcgggc acggtgcaga tgttccgcgc
     1381 gctggcggtg gagctaggcg agcgcaacgt gcgcgtcaac gtgatcgcgc ccggtgtggt
     1441 cgagacgccg ctgaccgccc agatcaagtc gcacccggag tggtaccggg cctatgccga
     1501 caagaccatg ctgcgccgct gggcccagcc gagcgagatg gtcggcgcgg tcgtctatct
     1561 ggcctcggac gccagctcct acgtcaccgg ctcctacctg ctggtcgacg ccggctggct
     1621 ggccgccgac ggccgcttca ccccaccgct gtcgtgagga gccccgcgtg acggcgcacg
     1681 agcatgtctc cctgacgcag actttggcgc gcatcgaccg cggtgagctg gtgcggctga
     1741 cccaggatct cgtccgcatc ccaagcgtcg tccggccgga cgacggcggc aacgagcaga
     1801 cggccgccga attcgtggct gggttccttg agcgtcacgg cctgccggtt tttcgcgacg
     1861 aggccgcgcc ggggcgtccc aacgtctggg ccgtgctgga gggcacgcag cagggcaaga
     1921 cgctcttgtt cgaggcgcac accgacgtcg tcaccgaggg agacccggcg agctggtcgc
     1981 agccgccctt cgccggcgcg gtggttggct cgcgcatcta cggtcgggga gcctgcgata
     2041 ccaagggcaa cctggccgcc gcggtgctgg cggtgctcgc gatcaagcgt gccgggctcg
     2101 acttccccgg gcgcatcgtc ctctgccacc ccgtcgacga agaggggctg atgctcggaa
     2161 tcaagcgctt catcgccgcc ggccacgccg agggggtcga cgcggccatc atctgcgagc
     2221 cggaggagaa ccagctctgc atcgcgcaga aaggggcgct gcgggtcgcg gtccgcttca
     2281 gcgggcgcat ggcccacgga gcgatgcctc tgagcggtgt caacccgatc acgcgcgccg
     2341 cgcggttcgt ggtggccgtc gaggcgctgg agcgggacga gatcgagcgt cacggccgtg
     2401 atccgctgct cggctacccc agcctgacgc cgaccgtcct gcgcgccccc gacggcggcg
     2461 agccgcagct caacgtcatc cccgccgcgg ctagcgtggc gctggatatc cgcactatcc
     2521 ccagtcaatc gcatcagggg ctgctggcac ggctgcgcga gtgcttggac tggctgcggg
     2581 ccgacgatcc gctgctgcgc gccgagctga ccgtgctcga cgaccgcccc tgcaccgaga
     2641 cgccccgcga cagcgccatc gtgcgcgccg ccagcgacgc ctatcgcacg attaccggca
     2701 gcgagccgcg cttcaacggc gtacccggcg cgaccgacgg caccttcctg cacgcctggg
     2761 ccggcatccc gatcgtgacc accggcgcgg gccagcgcac cgtgccccac cacgccgatg
     2821 agtgggtcga catcgacgag ctggagacca cgtgccgcct ctacgcggcc acggcgatgc
     2881 gctacctggg ggcggactaa ggcgtaaggt tatgtgaagt atccggtttg aggatcgtgc
     2941 gtcggggtcg catgcggccg atgggagatc cttcgccgcg gctcagcgcc tgccctgagt
     3001 cgcggcgaag ggaggacagg ggaaggggcg ttgacttccc tgtcagcctg aggccgtagc
     3061 cgaaggatct cccatcggtc gctcgcggca accggtatcc acagctgccg atcagccaac
     3121 tcggggcacg ccggcgtctt cctcggtcag cgcctgcacc tcctggcgca gcgaatggac
     3181 ctggcgcccc agcgaccaga tgtagcccgc gatcacgacc caactgatca ggaacgcggc
     3241 gaaaaggtag ccgagattgt ccgtcatccg agcgacactc cctcttgcac ggctggttgt
     3301 tgcgactgcg gctgcgacgt cgccggcgta tccgcctgcg actggcgctc gtactcggcg
     3361 agctggtagt cgatcaagcg gtccttggcc tgctcgatgg cccatttctg ctgcagtagg
     3421 aaggcgtaga ggagcgtgaa ggctaccagc gacaccatca acgccagcag catgctcccc
     3481 ggcatggccg cgtgctccag cgaaacgacc tgggtgggat gcagcgtgcg ccaccactgg
     3541 accgacaggt aattgatcgg cacgtcgatg aaggccacga tgccgagcac cgccgcgaag
     3601 cgcgccgcgc gctcgcgctc tccagcgtag gcccgcagca tgaaatagcc gaggtacatg
     3661 aaccagagga tcagcgtggt cgtcaggcgc gcgtcccagg tccaccaggt gccccagatc
     3721 ggccggcccc agagcgagcc ggtgatcagc accagcgtcg tcagcaccag cccgatctca
     3781 gctgaggcgc gccccagttg atcccagaag accgagcgct tccagaggta gccgatgccg
     3841 gccaggaaga ccacgaagaa ggccaggtag gcgtcccagg ccatcggcac gtggatgtag
     3901 aagatgcgct gcgcattgcc ctgcaccgcg tcggtcggcg catagatgaa catcaggtac
     3961 aggctcagcg ccatcgtcag cccggtgagc ccgccgagga tcggtcccca accgggtgct
     4021 ccgccctcac tcttcatgtg cccctactcc tccaccacgc gatcgaagac cagaaacgcg
     4081 atcgcgacaa acagcacgtc gaagccgatc agcaggttga tccagtcgag cagcccgtcg
     4141 ccctggttgg tcagcaccag cgtggtcgca cgcaccgtgg cgatgaccac cggcagcgcc
     4201 agcggaaaga ggagcaccgg cagcatcacc tcgcgcgccc gcgtgctggc cgccatcacc
     4261 gccagcagcg tgccgaccgc cgcgaaccca atggtgccaa gcgccagtac cagaaacaat
     4321 agcgggcgca gcaccggcag atcgaagagc gccccgaaga ccggcacggc cacgatctcc
     4381 accagcccga tgaagatcaa gttgcccacc agcttgccca ggtagatcac cccgcggtcg
     4441 accgggcata gcagcaggcc gtcgaacgag ccgcggtcgc gctccagcgc caccgcgcgc
     4501 cccagcccca gcatgcccgc gaaactgaag gccacccaga gcgcgcccgc gccgctcgga
     4561 gctttggcca cgccggtcag gtcgaaggca aagttgaagg tgacaatcgt cagcacggcg
     4621 aagacgaaca tgccgagaaa gagttctttg ccgcgcagct cggtcagcag gtccttccag
     4681 accagcgtcc cgatcttgcc cagcgcgttc acgagggcac cgccagccgc tgatcgagct
     4741 gccgctcgtc gacggcgtcc accggcgcgt cgagcgtcaa gcgaccctgc gccaggagca
     4801 gcacgcgatc ggcacaaccc agcccgaagc gcaggtcgtg ggtcgtcagc agcacgctcc
     4861 cgccggccgc ggcgtgctcg ccgatcagcc gctgcagcag gcgcatcccc tcgcggtcga
     4921 gcccggtgtc cggctcgtcg aacagcagca ccgccggttg gtgcaggata gcgcggccca
     4981 tggccagccg ctgctgcatg ccgcgcgaga aggtgtgcac ccggtcgcca ctccgcgctc
     5041 gcagcccgac cagccccagc acctgctcga tgcgccgctc cgcctccggc acgtcgtaca
     5101 tacgcgcgaa gaagcgcagg ttctcctcgg cggtcaggtc gtcgtagagg tagggctggt
     5161 gggccacgac ccccaacgcg cgacgtgcac gccgggcatc acgctcgact ggaaccgtct
     5221 caatcaggat gcgcccggtc gtcgggcggg ccagcgtcgc caggatacgg aggagcgtcg
     5281 tcttgcccgc gccgttggca ccgaagagcg cgacccgttc gcccggcgcc agcgtcaggt
     5341 cgagcggccg gagcaccatg cgattgtcga agcgcttgct gacacgctcg gcttgcaggc
     5401 gtggtgcggg ggcacgcgat gtccctgccg gcgcttcctg agcaatcggg atggattgca
     5461 gctcattcac ggatcgcctt gtgcctggcc cggctgcccc tcgcgcgcaa ccagcgcgcg
     5521 cagcttcaag atctcgactt ccagctcatc gtcgagattg ccggtctcca ggttcagatc
     5581 gcgcgtcgcc agcgccgcct cgcgcaggaa gcgcccgcgt gtctcgcgat actcctcgtc
     5641 gccgaccttg cccgacgcat ggtcgaaatc gacgtcgcgg atcgcctcga tcagatactg
     5701 gcggcgagcg cgcaggtccg ccagctgcgc cggcagcgcg acggccgctt cctgctcgcc
     5761 gccgagcagc ggctccagca cgaagatgaa aaccgcgatc gagacggcgg ccagcgccag
     5821 gtatccgagc atcacatccc ctgatccgcg aggagctctt gccccacttg ctcctctaat
     5881 cgccgcagct cgtcgtcatc gacgctcggc tccggcaggc tcctgggccg gctcatgata
     5941 tagagcgcca ggatgatggc gccgaaggcc agcccggcca ccggcatcca ccagagggtg
     6001 tggtagaagc cctgcttcgg cggctcgcgc aggatgctga ccccgtagcg ctcgacgaag
     6061 tattccttga tctggtcggg cgactgcccc tgatcgagct gctgctggat cacctgccgc
     6121 atctcgaccg cgatgctcgc ctgcgagtcg cgcacgctct cgccggagca gaccgggcag
     6181 ttgagcccgt tggcgatctt gagcgcctcg ggtgagagcg gctcgtcggc cgccgccgcc
     6241 agcggcagta ggagcaacag cacggcgacc gcgaacgcgg tactgaggct acgcttcaac
     6301 tggcaccgcc tctcgctcgc gcacccgccg cgtcgcgcgc cgggcgaccg gccgcgccac
     6361 cggccagagc agcgtcaccg tgccgagcac gtagacgatc gcaccccacc agatccagct
     6421 taccagcggg ttgatgtaga cgcgcaggct ggcggtgctg tcggtgtccc agcgatcgag
     6481 caccacgtag acgtcgttga ggccgacggt cgcgatcccc atgcgggcgg tcggctggct
     6541 ctcccagccg gtgtagaaga agcgtttggt ctccaccgtc gcttgatgcg agccgccctt
     6601 ggagacgtcg accacggcgg agacgacctc gccctcgttg gtggtggtgt cgtgcagcgc
     6661 ggcataggtc agggtgaagc ccgcgacctg gcccgtctga cccggcttga gcgtgaattg
     6721 ccgctcgctc tggaagaagt gcgagccgat gatgccgacc gccatgatcg cgatcgccag
     6781 gtggacgatg tagccgccgt agcggcgatt attacgccgc accagcatcg ccagcgcgtg
     6841 caggtagttc tcgtgcatgg tgcgccgccg cacccgcgcc ccgcgcgcgt actccaccac
     6901 caccgaggtg acggtgaaga cggcggcgcc cagcccgagc gacgccagcg gattgcccag
     6961 gatcgcctgg aagaccggga ccgaggccac cccgagcagg atcggccaga gcaggttgcg
     7021 cagcagcagc cgccccggcg agcggcgcca ggcgagcatc ggcccgatgc ccatcaagat
     7081 cagcacgccg agcatcagcg gcccatcgac ctggttgtag aaggggggcc cgacggtcag
     7141 gcgggtgttg cggaagagct cgctgatcag cgggaagacg gtgccccaga aggtcgcgaa
     7201 ggccagcgcg gtgaagagga ggttgttcag caggaagccc gactcgcgcg agacgaccgc
     7261 ctcgatggcg cggtcggcct tgagcccgcg gaagcggtag gccaggaagc cggtcgcgac
     7321 cacgatcacg atcgccagat agcccaggaa ccagccgccg atcggcgagg tggcgaaggt
     7381 atgcaccgac gagatcagcc cgctacgcac ggtgaaagtg gcgaagaccg ccaggatgta
     7441 cgaggccacg atcagcgaca tgttccagac cttgagcatg ccgcgccgct cttgcacgat
     7501 gatcgagtgg atgaaggccg tcaccgtcaa ccaggggatc agcgccacgt tctcgaccgg
     7561 atcccagccc cagtagccgc cccagccgag cacgtggtag gcccaccagg cgcccagcac
     7621 cagcccggtg cccaggatgc cgaaggagac cagcgcccag cgccggatag cccggatcca
     7681 gtcgtcgccc agccgcccgc tgatcatggc cgccatggcg aagccgaagg gcacggtgaa
     7741 gctggcgaag ccgcccagca ggaagggcgg atgcatcatc atgccggggt cacgcaagag
     7801 cgggttgagg ccgaggccgt cgctcggcgt cgtgggcgag agcgtgaagg ggctggcgac
     7861 gaagttgagc atgaccagga agaagagctc ggtcgtcagc accgtcgccg cgacataggg
     7921 gatcagcgcc cggtcgcgct tgcggtgcag gaagatcgcc agcgacgaga gcagcccggc
     7981 gatcatcgac cagtagagca ggctgccttc ttgcccgccg tagaaggcgg ccaggacgta
     8041 atggatcggc atctcgcggc tggagcggcc ggctacgtag acgagctgaa agtcgtgggt
     8101 caggaaggcg gtgatgagcg caccgaccgc gaccacgatc agcgcggtca cgccccagac
     8161 cgcgcggatg ccgctctgga tcagctccga cgattggcgc ctggctccca gcaccgagac
     8221 cacgatgccg tagagcgtaa tcgcgaaggc gacgatgagc gcgccggagc cgagttgtgc
     8281 caagatcgac ctctcttact gacttggttt ggcggtgaag cgcgaggggc acttggtgag
     8341 cagcgtgctc gcctcgaacg tgccgttggt gtggtacgtc ccttcgatca ccacctgcgt
     8401 gttgtccgtg aatatgtcgg gcagcacacc gttgtaaacc accgggatcg agtgcgtgcc
     8461 gtccccgacc acgaagcgga tcgggtcgcc cgcgttcccc ttatcgatcg tgcccggcac
     8521 cacgtcaccg ccgacgcgca cgcgcttgcc gtccatctgc accgactgct gctgcagctc
     8581 gccgacggtc acgaaatacg aggacgcgga accctgcatg gccgagaaga tgaggtagcc
     8641 aaccgccagc aggatggcga taccggcgat gaggagcttc gggttgaccc aggagaagcg
     8701 cctctcggcg gccgcggcca ccggctgcgc catcggagcc cccgcttcca cgatccagtc
     8761 tccctcccag tcagcgaggt cagcgttggc cgatcccggt cgggtagctc gccgccagcg
     8821 cgatgcccag tacgaaggcc gtcccgccga gcatgagtac caggagcgac cgattgactc
     8881 tggccgcgcg tcccgagcgc gccggctgcc ggcgccccgc gacgcgccgg gtgaagacgc
     8941 cggtgagcca gaaggcggtc agcgccgcca cgatcttcac ccccagcacg atgacgtaga
     9001 ccagc
//
LOCUS       DASUBI010000171         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1072308, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000171 DASUBI010000000
VERSION     DASUBI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072308"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /gene="thiS"
                     /locus_tag="VFU72_03355"
     CDS             complement(<1..152)
                     /gene="thiS"
                     /locus_tag="VFU72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU5422554.1"
                     /translation="MELTINGKTARIEGVSTVAELIAARGLKQGLVAVEQNGEIVPRQ
                     EFAHRS"
     gene            complement(143..775)
                     /locus_tag="VFU72_03360"
     CDS             complement(143..775)
                     /locus_tag="VFU72_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5422555.1"
                     /translation="MTTVGRLHLISDRARCPLERLPEVAALAVRGGVDTVHLREKDLP
                     GGVLLELAGRLREAINGRAALIVNERVDVAVLAGADGVQLGEAGLPIDGTRRLLGDRL
                     IGRSVHDRAGAWKAGMQRADFLLAGHVYETGSKAGQPGRGLRMLGELAGVSHLPVVAI
                     GGITPERVVPVLRAGAWGVAVLSGILAADEPEAAARRYREVIEEELTQWS"
     gene            complement(772..1440)
                     /gene="thiE"
                     /locus_tag="VFU72_03365"
     CDS             complement(772..1440)
                     /gene="thiE"
                     /locus_tag="VFU72_03365"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5422556.1"
                     /translation="MDLEAVRAGLGNALRLYVLTDRRLAGGRAEAEVVRAALAGGATA
                     IQLRWKTGPLREALAAGREVRELCRAAGVLFVVNDRIDLALALEADAVHIGEHDLPLP
                     EARALVGERMLIGYSPPTLVEAIQAQRLGADYLGIGPVFSTRTKADAGEPVGLKRIHD
                     VAESVTLPFVGIGGIDADNAASVIEAGADGVAVISAVVAAPDIEAAARRLRHTVDAAL
                     DLRL"
     gene            complement(1441..2235)
                     /locus_tag="VFU72_03370"
     CDS             complement(1441..2235)
                     /locus_tag="VFU72_03370"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU5422557.1"
                     /translation="MHASNGARDGGLTIAGKTFSSRLILGTGKYRSSEEMVAAFAASG
                     AEMITVALRRIDFDDPKSRSILEDVDWTRYQILPNTAGCQTAEEAVRVARLARSLGLS
                     DWVKLEVIPDSKYLLPDPIGTLEAARQLVDEGFTVLPYINADPVLARRLQEAGTATVM
                     PLASPIGSGQGIPDYRQLQIIIEQATVPVVVDAGIGAPSDAALAMELGADACLVNTAI
                     AKAEDPAAMARAMRLGADAGRLAFQAGRIPKLAYAAASSPLEGVVR"
BASE COUNT          330 a          891 c          697 g          331 t
ORIGIN      
        1 aacgaccggt gtgcgaattc ctggcgcggc acgatctcgc cattctgctc gaccgccact
       61 agcccctgct tcagcccgcg cgccgcgatc aattcggcga ccgtgctgac cccttcgatt
      121 cgtgccgtct tcccgttgat cgtcagctcc actgcgtcag ctcctcctcg attacctcac
      181 gatagcggcg cgccgccgcc tcgggctcgt ccgccgccag gatgccggag agcaccgcca
      241 cgccccacgc gcccgcgcgc agcaccggca cgacccgctc cggcgtgatg ccgccgatcg
      301 ccaccaccgg caggtgcgat acgccggcca gctcccctag catccgcagc ccacgccctg
      361 gctgccctgc cttgctgccg gtctcgtaga catgccccgc cagcaggaaa tcggccctct
      421 gcatgccggc cttccaggcg ccggcccgat catggaccga gcgcccgatc aaccggtctc
      481 ccagcaggcg gcgcgtacca tcgatcggca gtcccgcctc acccaactga accccgtcgg
      541 cccccgcgag caccgccacg tcgacgcgct cgttgacaat cagcgccgcg cgcccattga
      601 tcgcctcgcg cagccgtccg gccagctcca gcaacacgcc gcctggcaga tccttctcgc
      661 gcaggtgcac cgtatcgaca ccgccgcgca ccgccagcgc cgccacctcc ggcagccgtt
      721 ccagcggaca gcgagcgcgg tcgctgatca ggtgcagccg gccgaccgtg gtcatagccg
      781 caggtcgagc gcggcgtcga ccgtgtgacg cagccgcctg gcagccgcct cgatgtccgg
      841 cgctgccacc acggccgaga tcaccgccac accatcggcc ccagcctcga tcaccgaggc
      901 ggcgttatcg gcgtcgatgc cgccgatccc gacgaaaggc agcgtgactg actcggctac
      961 gtcgtggatg cgcttgagtc cgaccggctc gccggcgtcg gccttggtgc gggtgctgaa
     1021 gaccgggccg atgccgagat agtcggctcc gaggcgttgg gcctggatcg cctcgaccag
     1081 cgtcggtggc gagtagccga tcagcatgcg ctctcccacc agcgcgcgcg cctccggcag
     1141 cggcaggtcg tgctcgccga tatggacggc gtcggcctcc agcgccagcg ccaggtcgat
     1201 gcggtcgttg accacgaaca gcacgcccgc cgcgcggcag agctcgcgca cctcacgccc
     1261 ggccgccagc gcctcgcgca gcggtccggt cttccagcga agctggatgg ccgtcgcgcc
     1321 gcccgccagc gcggcgcgca ctacctcggc ctccgcgcgc ccgcccgcca accggcgatc
     1381 ggtgagcacg tagagccgca gcgcgttgcc cagtcccgcc cgtaccgctt ccaaatccac
     1441 ctagcgcacc acgccttcga gcgggctgct ggccgcggca taggccagct tcgggatgcg
     1501 gccggcctgg aaggcgaggc gtccggcatc ggcccccagg cgcatcgccc gcgccatcgc
     1561 cgctggatcc tccgccttcg cgatcgccgt gttgaccagg caggcatcgg cgccgagctc
     1621 catcgccagc gcggcatcgg agggcgcgcc gataccggca tcgaccacca ccggcacggt
     1681 ggcctgctcg atgatgatct gcaactgccg gtagtcgggg atcccctgtc ccgaaccgat
     1741 cggcgaagcc agcggcatca cggtcgcggt gcccgcctcc tgcaaccgcc gcgccagcac
     1801 tgggtcggcg ttgatgtacg gcagcacggt gaacccttcg tcgacgagct gcctggccgc
     1861 ctccagcgtg ccgatcggat cgggcagcag atacttagaa tcggggatga cctccagctt
     1921 gacccaatcc gagagcccga gcgagcgcgc cagccgcgcc acgcgtacgg cctcctcggc
     1981 ggtctggcag cccgcggtgt tgggcaggat ctggtagcgc gtccaatcca cgtcttccag
     2041 aatgctgcgg ctcttgggat cgtcgaagtc gatgcgccgc agcgccaccg tgatcatctc
     2101 ggcgcccgac gccgcgaacg ctgccaccat ctcctcgctc gatcgatact tgccggtgcc
     2161 caggatcagc cgcgatgaga atgtcttacc ggcgatcgtc aagccgccgt cccgcgcccc
     2221 gttgctcgcg tgcattactc gctcccttc
//
LOCUS       DASUBI010000172         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_322728, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000172 DASUBI010000000
VERSION     DASUBI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322728"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFU72_03375"
     CDS             <1..423
                     /locus_tag="VFU72_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422558.1"
                     /translation="GNPVGDQYVSTGGTTVQYFENARFELNREGSVGLGRIGAEAFHQ
                     TVPRVDDPKSPNVAYFATTGHTLQGAFKTFWEQNGGLSVFGYPISEVRDEDGTKVQYF
                     ERARFELVPSGNSAQSTVRLTPLGAQMWASVQQTVVAH"
     gene            complement(420..791)
                     /locus_tag="VFU72_03380"
     CDS             complement(420..791)
                     /locus_tag="VFU72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422559.1"
                     /translation="MSAERKQQRVVVVGPCGAGKSTVVEQLRRWGYDAFTVSQEHSIV
                     PDLWRRRAPDALIYLHVEYPVLQARRGAAWPHALYERQMERLEDARRHATLVIDTGAA
                     GINETMARVVAALERSAARDS"
     gene            complement(842..1555)
                     /locus_tag="VFU72_03385"
     CDS             complement(842..1555)
                     /locus_tag="VFU72_03385"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU5422560.1"
                     /translation="MTAGIELAVAKTHRFGTRESGDTAEVVERPGGGISVVVVDGQGS
                     GVAAKRLSLMVAGKVMSMLSEGVRDGAVARAAHDFLFAQRGGRVSCALDIISVDQVTN
                     ELLLARNSEVPMLLARCAGLEELPDSGGRIGLYPRTRPSMLRFPAEPGLVAIVVSDGV
                     ASAGRRFGHTPGLSGLAAGLSFADTPAEQLADTLLRRAVEADRGHPRDDMTVVVVRLV
                     PPIVAYPVRRLALSLPVQE"
     gene            2279..>3458
                     /locus_tag="VFU72_03390"
     CDS             2279..>3458
                     /locus_tag="VFU72_03390"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU5422561.1"
                     /translation="MVPSERSLLLGVILVIFIVAGELFGAISLPTTSVKADVSISSAL
                     CFGAAITLGPMGALVVGVSKLAVEMYRRITPMKLAFNVSSYFITTFATGLAYVAVADP
                     HSSIIANPTNLLAAALAVLVFNVMNFSLAAAIFSQVASTSPLEMWIALARGNVFTILT
                     LPTLGMLVPILAQEQPLAILLMVVPLIGPYLSLRSYSQIHLETRRTVERLAGMLDQRD
                     PTTARHSRRVADYVEQILGEMDELTFEQREAILIAAPVHDIGKIGTSDLVLQKPGKLT
                     DEERDHINRHAADGGDILSNLSLYRHAAVIVRHHHERWDGKGYPDGLAGETIPIGARV
                     IAVADTFDAMTTDRPYRAGLTPQRALEEIQRCAGTQFDPRVAEIFLRLMSCYRPAVEL
                     P"
BASE COUNT          593 a         1198 c         1068 g          599 t
ORIGIN      
        1 ggcaaccccg tcggcgatca gtacgtcagc acgggaggca cgacagtcca gtatttcgaa
       61 aacgcgcgct tcgagctgaa ccgtgagggc tcggtcggcc tgggacggat cggcgcggag
      121 gcgttccacc agacggtgcc gcgcgtcgac gacccgaaga gcccgaacgt cgcctacttc
      181 gccaccaccg gtcacacgct ccagggcgcg ttcaagacgt tctgggagca gaacggtggc
      241 ctgtcggtct tcggttaccc gatcagcgag gtgcgcgatg aagacggcac caaggtgcaa
      301 tacttcgagc gcgcccgctt cgagctggtc cccagcggca actccgcgca gtcaaccgtg
      361 cgtctgacgc ccctcggcgc ccagatgtgg gcctcggtgc agcagactgt cgtcgcgcat
      421 taggagtcac gcgcagcgga ccgctcaagc gccgccacca cccgcgccat cgtctcattg
      481 atgccggccg cgccggtgtc gatcaccagg gtcgcgtgcc gccgggcgtc ttccaaccgc
      541 tccatctgcc gctcatagag cgcgtgcggc caagccgcgc cgcgccgcgc ctggagtacc
      601 gggtactcca catgcaggta gatcagtgcg tccggcgcgc gtcgccgcca gaggtcgggc
      661 acgatcgagt gctcctgact gaccgtgaat gcgtcgtagc cccagcgccg caactgctcg
      721 accacggtag atttgcccgc gccgcacggt ccgacgacta ccacccgttg ctgtttgcgt
      781 tccgcgctca tgccgcccgc gttccaaact acaccgtcgc cattcccgca ccgttgcggc
      841 actactcctg aaccgggagc gataatgcca gccgccgcac cggatatgcg acgatcggtg
      901 gcacgagccg gaccacgacg acggtcatgt cgtcgcgcgg gtgtccccga tcggcttcga
      961 ccgcgcggcg caggagcgtg tcggccaact gctccgccgg cgtatcggcg aatgacaatc
     1021 ccgcggccaa gccggacagc cccggcgtat gcccgaagcg ccgcccggcc gatgccaccc
     1081 cgtcgctgac cacgatggcg accagccccg gctccgctgg gaagcgcagc atgctcggtc
     1141 gagtccgggg ataaagcccg atcctgccgc cggaatccgg tagctcttcc aacccggcgc
     1201 aacgcgccag cagcataggc acctcgctat tgcgggccag cagtagctcg ttggtcacct
     1261 gatcgaccga gatgatatcc agcgcgcagg agacgcggcc gccgcgctgg gcaaagagga
     1321 agtcgtgcgc cgcacgcgcg accgcaccat cgcgcacacc ctcgctcaac attgacatga
     1381 ctttgcccgc taccatcagg ctcagccgtt tcgcggccac gccgcttccc tggccgtcga
     1441 cgaccaccac cgatataccg ccgccggggc gctcgaccac ctcggcagtg tctccgctct
     1501 cgcgcgtgcc gaacctatgc gtcttcgcaa ccgcgagctc gatgccagcg gtcatcacgg
     1561 agcgccttcg cgcggatgcg cggccggcag cacaggttga tcgcgtaatc gcacgacaac
     1621 ttccccaatg acggtgagat ccacgcgagt caggttgcct gtcaagagtc gcacggtcaa
     1681 gcagtgcatc gcaatccgag taccaacgtc ttgacctgaa cgtacgagcg tactattata
     1741 ggcatagcag cgggtacgcg tgtactcaga caaagcggac tgtttggcta gagccaaggg
     1801 gttgtgtgtc tgagcgccgg accacagccg ctaatgacgc tgagagagat ccagtcgggt
     1861 ggggtcgacg ggagtaggga cagggaaggg tcatgattag ctggcgctaa ctcatcggcc
     1921 gggatcaagc gcttgccagc cgtgaggcgc aagccgcgct ccccaatgac ctagactcgt
     1981 ccgggttccc gctagcgact gaggacgcct gctagcgaga gcccggtcgc tgttggtctc
     2041 ggtaaccccc cgtgcagtac agatcgccct gtccgttcag gaccaaaagg taccgacgca
     2101 ccggcgcggc ggcactagac tacgcttgac acgtgtcgat cgcaccgatc ggccggtcgg
     2161 tatctgccaa gcggctcggg agtcctgaat gcgacgaggc tcgcgcgtcc gtgcgtccgt
     2221 cctgacagtg acattgctgg cgtttctctg cgcatctctc ggtttgatcc actggggcgt
     2281 ggttccgagc gagcgctccc tgctccttgg cgttatcctg gtgatcttta tcgtggccgg
     2341 cgagctcttc ggcgcgatca gcttgcccac gacctcggtc aaagccgacg tgtccatttc
     2401 ttcggccctc tgtttcggcg cggcgattac cctcgggccg atgggagcgc tagtcgtcgg
     2461 cgtgtccaag ctcgccgtcg agatgtaccg gcggataacg ccgatgaagc tggccttcaa
     2521 cgtttcaagt tacttcatca ccacgtttgc gaccggactc gcgtatgtgg ccgtggccga
     2581 tccgcacagc tccatcatcg ccaatccgac caatctgctg gccgccgcgc tcgcggttct
     2641 cgtcttcaac gtgatgaact tcagcctcgc ggcggcaatc ttctcccagg tggcatccac
     2701 ttccccgctg gagatgtgga tcgcgctggc gcgtggcaat gtcttcacga tcttgaccct
     2761 cccgacgctc ggcatgctgg tgccgatcct ggcgcaggag caacccctgg cgatcctctt
     2821 gatggtagta ccgctgattg gtccctatct ctcgctgcgc agctacagcc agattcacct
     2881 ggagacgcgc cgcacggtcg agcgcctggc cggcatgctc gaccagcgcg accccaccac
     2941 cgcgcgccat tcgcgtcgcg tcgccgacta cgtcgagcag atcttggggg agatggatga
     3001 gctgaccttc gagcagcgcg aggccatcct gatcgccgcg ccggtccacg acatcggcaa
     3061 gatcggcacg agcgatctgg tgctgcagaa gccgggcaag ctgaccgacg aagagcgtga
     3121 ccatatcaac cgccacgcgg ccgacggcgg cgacattctc tccaatctct cgctctatcg
     3181 tcacgcggct gtcatcgtac gccaccatca cgagcgctgg gacggcaagg gctacccgga
     3241 cgggctggcg ggtgagacca tcccgatcgg cgcgcgggtc atcgccgtcg ccgatacgtt
     3301 cgacgccatg accaccgacc gcccctaccg cgccggcctc acgccgcagc gggcgctgga
     3361 agagatccag cgctgtgccg gcacgcagtt cgatcctcgt gtcgcggaga tcttcctgcg
     3421 cctgatgtcg tgctaccggc cggcggtcga gttgccgt
//
LOCUS       DASUBI010000173         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1357918, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000173 DASUBI010000000
VERSION     DASUBI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357918"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFU72_03395"
     CDS             <1..402
                     /locus_tag="VFU72_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5422562.1"
                     /translation="LRTRLDCERLVVTLGPEGAALVERDGGYLQVPAAARRQVFDVTG
                     AGDTVIAVMTAALAAGATSPAALRLSQVAAGLVISLWGNAQVSAEEIAAALDEGDPPS
                     AEEPSSPRPFSQSWEKGSHDRSPSPRIGRGS"
     gene            406..1350
                     /gene="cofD"
                     /locus_tag="VFU72_03400"
     CDS             406..1350
                     /gene="cofD"
                     /locus_tag="VFU72_03400"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEU5422563.1"
                     /translation="MRASIVALAGGVGGAKLAHGLALARPDRPLSVIVNTGDDFTLHG
                     LHIAPDLDTVMYTLAGLANPETGWGIQGDSFATLEMLERYGAESWFRLGDRDFATHIQ
                     RTLRLRQGQPLSAVTAELAGALGVRAALLPMCDEPVATLVETPAGVLDFQDYFVRRHQ
                     QDDVLGITLRGIELARPLSQVLDAVDAAEAIVLCPSNPIVSIGPILALPGLRARLKAA
                     PAPVVAVSPIIGGQALKGPADRMLATLGHEVSAAGVAALYNGLVDGMVIDEQDAALAG
                     RIEALGMAVEVAQTIMRDEADRRGLAERVLAFAERLRR"
     gene            1347..1967
                     /gene="cofC"
                     /locus_tag="VFU72_03405"
     CDS             1347..1967
                     /gene="cofC"
                     /locus_tag="VFU72_03405"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEU5422564.1"
                     /translation="MTTVALVPVQRLETAKSRLAGRLCPAARRELVLALLERVLGALA
                     GAERVDAVLVVSPDPAVLAFAAASGALPLAQPGSGLNAALKLGRSEALRRGADRLLIV
                     LGDLPLLTPAEVDALLAHPEAVVLAPDRHERGTNLLALRPPAALPPAFGPDSLRAHRL
                     AAQQRGLPLQEYRSRATALDLDTPEDLALLVEREDLTVPHCVHPRG"
     gene            2436..>3028
                     /locus_tag="VFU72_03410"
     CDS             2436..>3028
                     /locus_tag="VFU72_03410"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5422565.1"
                     /translation="MEEQFMPSEPIAIPPEYSRRIRELAGEVGVAWLARLPGVVEECA
                     ARWSLRLGRPFPPSYNYVVPGWRGGEPVVLKIGLPGAGLVREIEALERFDGRGVARLL
                     ALDRERGALLLERLVPGTSLATLGDDERATVIAADVLRRLWRPAPAAHGLTSLADRAA
                     DLSRIRVRFGGGSGPLPEGLVARAESLFAELIASAAPP"
BASE COUNT          374 a         1015 c         1192 g          447 t
ORIGIN      
        1 ctgcgcacgc ggctcgactg cgagcggctg gtggtgacgc ttgggccgga gggcgcggcg
       61 ctggtcgagc gcgacggcgg ttacctacag gtgccggcgg cggcgcggcg gcaggtcttc
      121 gacgtgaccg gcgcgggcga cacggtgatc gcggtgatga cggcggcgct ggcggccggc
      181 gccacctcac cggcggcgct gcggctgtcg caggtggcgg cggggctggt gatcagcctg
      241 tggggcaacg cgcaggtctc ggccgaggag atcgcggcgg cgctggacga gggcgatccg
      301 ccctcagcgg aggagccctc atccccccgc cccttctccc aatcctggga gaaggggagt
      361 catgaccgtt ccccttcgcc cagaattggg agagggagct agggggtgag ggcgtcgatc
      421 gtagcgctgg cggggggcgt gggcggcgcc aagctggcgc acgggctggc gctggcgcga
      481 ccggatcgtc ccctgagcgt gatcgtcaac accggcgacg acttcacgct gcacgggctg
      541 catatcgcgc ccgacctcga tacggtgatg tacaccctgg ccggcctcgc caacccggag
      601 accggctggg gcattcaggg cgacagcttc gccacgctgg agatgcttga gcgctacggc
      661 gccgagagct ggttccgcct gggcgaccgc gacttcgcga cgcacatcca gcgcacgcta
      721 cggctgcgcc agggccagcc gctcagcgcc gtgaccgccg aactggccgg cgcgctgggc
      781 gtgcgcgccg cgctgctgcc gatgtgcgac gagccggtgg ccacgctggt cgagacgccc
      841 gcgggcgtgc tcgacttcca ggactacttc gtgcgccgcc accagcagga cgacgtgctg
      901 ggcatcacgc tgcgcgggat cgagctggcg cggccgctgt cgcaagtcct ggacgccgtc
      961 gacgcggctg aggcgatcgt gctctgcccc tccaacccga tcgtcagcat cggcccgatc
     1021 ctcgcgctgc cggggctgcg cgcacgactg aaggcggccc ccgcgccggt cgtggcggtc
     1081 agcccgatca tcggcggcca ggcgctcaag ggtccggccg accgcatgct ggcgacgctg
     1141 gggcacgagg tctcggcggc gggcgtggcc gcgctctaca acgggctggt cgacggcatg
     1201 gtgatcgacg agcaggacgc ggcgctggct gggcggatcg aggcgctcgg gatggcggtc
     1261 gaggtggcgc agacgatcat gcgcgacgag gccgatcggc gcgggctggc cgagcgcgtg
     1321 ctggccttcg cggagcgcct gcgccggtga cgaccgtcgc gctggtgccg gtgcagcggc
     1381 tggagacggc caagagccgc ctggccggcc ggctctgccc ggcggcgcgg cgcgagttgg
     1441 tgctggcgct gctggagcgg gtgctgggcg ccctggccgg cgcggagcgc gtcgacgccg
     1501 tgctggtggt cagtcccgat ccggcggttc tggcgttcgc cgcggccagc ggggcgctgc
     1561 cgctggcgca gccggggagc gggctgaacg cggcgctgaa gctggggcgc tccgaggcgc
     1621 tgcggcgcgg cgcggatcgg ctcctgatcg tgctcggcga cctgccgctg ctcacgccgg
     1681 ccgaggtcga cgcgctgctg gcacacccgg aggcagtggt gctggcgccc gaccgccacg
     1741 agcgcggcac gaacctgctg gcactgcgcc cgcccgccgc gctgccgccc gccttcggcc
     1801 ccgacagcct gcgcgcccac cggctggccg cccagcagcg cggcctcccg ctccaggagt
     1861 atcgctcccg cgccaccgcg cttgacctcg acacgccgga ggacctggcg ctgctggtgg
     1921 agcgtgagga tttgaccgtg ccgcattgcg tccacccgag gggctaagac tccaacaaag
     1981 gttggattgt ttcgagcacg acttgctcgg tcatggctcc tcgcgctggg agcggtggcg
     2041 ccagactgtg ccgtagtctc tataggatcg gtgcctgggg ccgcgcgaag ttcatggacg
     2101 gtttgccgtt gtcccggagc gtcggtgccg ggtggcgccc gagaatggaa ttctcgggct
     2161 gagtccgcga agcccactga aggggctggg tttacgcttg actatgctgg cgcctttgca
     2221 gccaaatcgc tgcctcgccg cctggtgatc gcgcgatagc gaaaacattg tgacagccgt
     2281 tctcagcccc tttagtgggc ttcgtggact cagcccgaga atggaattct cgggcgccac
     2341 gcggcgctag ggtctcgggg tttacggcga acgacccacc cgaccccgcg cgaccggcgg
     2401 tgcgaggccc ccaaggggaa cggcacgagc cgtgcatgga agagcaattc atgcccagcg
     2461 aaccgatcgc catccctccc gaatactcgc gacgaatccg cgagctggcg ggggaggtgg
     2521 gcgtggcgtg gctggcgcgg ctgccggggg tcgtggagga atgcgcggcg cgctggtcgc
     2581 tgcggctggg gcgtccgttt ccgccctcct acaactacgt ggtgccgggt tggcgcgggg
     2641 gcgagccggt ggtgctcaaa atcgggctgc cgggggcggg gctggtgcgc gagatcgagg
     2701 cgctggagcg gttcgatggc cgcggcgtgg cgcggctgct ggcgctggac cgggagcggg
     2761 gcgccttgct gctggagcga ctggtgccgg gtacgtcgct ggcaaccctc ggcgatgacg
     2821 agcgcgcgac cgtgatcgcg gcggacgtgc tgcggcgctt gtggcgtccg gcgccggcag
     2881 cgcacggctt gacctcgctg gccgaccggg cggccgacct gtcccgcatc cgggtgcgct
     2941 ttgggggcgg cagcgggccg ctgccggagg ggctggtggc gcgggccgag tcgctcttcg
     3001 ccgagttgat cgcctctgcc gcaccgcc
//
LOCUS       DASUBI010000174         5388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1003635, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000174 DASUBI010000000
VERSION     DASUBI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003635"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFU72_03415"
     CDS             <1..399
                     /locus_tag="VFU72_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5422566.1"
                     /translation="RTMIMGEPTPEQARFFELMLQAQTLALDTIRPGIPCAAVDQAVK
                     DFFKEHDIERYWRHHSGHAKSTLIHEAPFLDVGDQRLIEEGMVFTVEPGIYVPDFAGF
                     RHSDTVAVTAAGIEMITYYPRDLEHLTIPV"
     gene            476..1411
                     /locus_tag="VFU72_03420"
     CDS             476..1411
                     /locus_tag="VFU72_03420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5422567.1"
                     /translation="MPRHVYLHPDAADPVLPDTTVLALVRRHVPAAQRLVAVEESGGE
                     ARTYLVDGELILKVQRPQQLRPLTSLAKEVFFLQRLAALPAECRISVPRVVGHGRVEP
                     GIEYTLLTRMPGRALRTTALAPAARQAALGELGRTLRRLHALPVEPFIQSGIVPGDLT
                     FMDTMTRIAEPLLDRSRAVRERGLAWNFPLSIEQIAGRALSSLPHARERAALHSNPYE
                     EHTFVDPDTGVFTGLIDFGDAYISHPALDIRRWNRPADRDAIMAGYQEAGRVSDDWLA
                     VWRALMIVADVDTMVRVPERADSARQDLETLLGEL"
     gene            1425..2522
                     /locus_tag="VFU72_03425"
     CDS             1425..2522
                     /locus_tag="VFU72_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422568.1"
                     /translation="MNPAVDLQHIAKIYAGQEVSKRGTSLQMSGPALWFRNLSRALQL
                     TASSSPPVQALSDVSLRIAPGEIFGLVGRNGSGKTTLIKIMAGLIRPTSGDGSVVGFS
                     LQEPQEIRKRVSYVSTTGWMGLEWPLTAEDNMRYFALLCGMPDRLARQRTEQALRDVE
                     LWEERTKYPSQLSNGMRQRVILARALLFHTPLVLLDEPTVGLDPLTTRSVLELIRGPL
                     RERGQTIVLTDHQSAEMEAVAGRIAILREGRIWRQGTPRELVARLAGLTVVEIRTEEM
                     DLPSGPPPEIVVAAQPLERPGALGERVWRVHAHRCPDALEQVVDWIEQPAGRIVFLAE
                     SEPTLRDLLALPEELVPGEAAASDVDLAAGR"
     gene            2519..3478
                     /locus_tag="VFU72_03430"
     CDS             2519..3478
                     /locus_tag="VFU72_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422569.1"
                     /translation="MSHSRKPAIAGAPPGVPARATGAAGRRSLWREFVALWAFGLREY
                     RIWQSYRVNQVLWLTNLFVTTYLFFLLGKTLAGDAASVIPAAYGTNYMSFIVVGVTIN
                     VFINTNLVDPYIRIQRAYFQGTMDLFLLSPMSIYTPLLGLMSKSAIDDYPRLFFTFGF
                     GMLLFGATFTFQYVWLALFFTALFLAAGFGIGLLSASSFHLLDIKKGTEPIGFVVQGL
                     LATLVAGTYYPVTVLPLPLRWLACLIPHTYAFDALRRLLDPNAQLGQPVLPLQHALPG
                     LSPIMVDGLALGLLAVTLLPLGFYLYGRGIERARGNGTLTRWQ"
     gene            3469..4443
                     /locus_tag="VFU72_03435"
     CDS             3469..4443
                     /locus_tag="VFU72_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5422570.1"
                     /translation="MAVIDVHGLTKRYRQGRGEPVTVLRDVDLRLEEGTVLGLLGPNG
                     AGKTTLIKILAGLLRPDAGGGSVLGFDLGRQHAEIRARVSLVAPTADVGIDNNLTVRQ
                     NLTFWAPIYGLRGAAARTRIDALLAQLELTDKADAWPMHISAGQRQRLALARSLIAEN
                     RLVFLDEPTNKLDLEGVRLVRTMITQLNRELGVTIVLTTHVMEEAEELCGEIALLHQG
                     ALVAHAPTGQLTWSLALARPVTITLRGTNPPDPNWLACLRAVPGVTNVHAPTRDAAGR
                     VVLAAESTDLRLTTPALLAYLRASGVALASMRAEPVTLDDVFSALGSL"
     gene            4449..5327
                     /locus_tag="VFU72_03440"
     CDS             4449..5327
                     /locus_tag="VFU72_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422571.1"
                     /translation="MPLPVQAREHQQGGALAFLRACWASGWLEYRNLRYYPTNLGLAA
                     VQELTTVGVWYFVARFLHSAADASVAQYGGNYLAFVVLGVLLNQVALAALTGPFQTVS
                     EAFWDKRLETYRLSIHGIWANVTGRLAWRVAFSSVLQFGALVILIAIGAIRIHAGIHV
                     ELALLALLLLVVANAGLGIAGASLFFLLEVKNGSDPITWAYKYLVMLAAGLYVPVVLL
                     PVWLRAAGAALPQTYAFAAERSIVLTGAGWSQGSVGHDLIGLALGALVALGAGCVMLN
                     LALRRAERQGGIGVVV"
BASE COUNT          806 a         1901 c         1743 g          938 t
ORIGIN      
        1 cggaccatga tcatgggcga gccgacgccg gagcaggcac gcttcttcga gctgatgctg
       61 caggcgcaga cgctggcgct cgacacgatc cggcccggca tcccctgcgc ggccgtcgac
      121 caggcggtca aagacttctt caaggagcac gacatcgaac gctactggcg gcaccacagt
      181 gggcacgcca agtcgaccct gatccacgag gcgcccttcc tcgatgtcgg cgaccagcgc
      241 ctgatcgagg agggcatggt cttcaccgtc gagccgggca tctacgtccc ggatttcgcc
      301 ggcttccgcc actccgacac cgtcgcggtc accgccgccg gcatcgagat gatcacctac
      361 tacccgcgcg acctggagca cctgacgatt ccggtctaac gtatgctaca gagcgtcata
      421 ccctatgccc ggtaccgcat ccgctggcaa tgcatgtccg tgcgaggagg ggtcggtgcc
      481 acgtcacgtg tatctgcatc ccgacgcggc agatccggtt ctgccggaca ccacggtgct
      541 ggcgctcgtg cgacgacatg tgcccgcggc ccagcgcctg gtggcggtgg aggaatcggg
      601 cggtgaggct cgcacgtatc tggtcgacgg cgagctcatc ctcaaggtgc aacgcccgca
      661 gcaactgcgg ccgctaacga gtctggccaa ggaggtcttc tttctccagc ggctcgcggc
      721 gctgccggcg gagtgccgga tcagcgtccc ccgcgtagtc gggcacgggc gagtggaacc
      781 tggcatcgaa tacaccctgc tgacacgcat gccagggagg gcgctgcgca cgacggccct
      841 ggctccggct gcccggcaag cggccctcgg cgagctgggc cgtacgctgc gccgcctgca
      901 cgcgctgccc gtcgagccat tcattcagag cggcatcgtg ccgggagacc tgaccttcat
      961 ggacaccatg acccgcatcg ccgagccttt gctcgatcgg tcgcgtgcgg tgcgcgagcg
     1021 cgggctggcc tggaacttcc ccctctcgat cgagcagatc gccggtcgtg cgctgtcatc
     1081 gctcccccac gcgcgcgagc gcgccgcgtt gcactccaac ccttacgagg agcatacctt
     1141 cgtcgacccc gacacggggg tctttaccgg gctgatcgat ttcggtgacg cctacatcag
     1201 ccacccggcc ttggacatcc gccgctggaa ccggccagcc gaccgcgacg cgatcatggc
     1261 cggctaccag gaggccggtc gcgtcagtga cgactggctg gccgtctggc gggcgctcat
     1321 gatcgtggcc gacgtggata ccatggtgcg cgttccggaa cgggccgact cggcccgcca
     1381 ggacctcgag acgctcctgg gcgagctcta gcggagccat gccgatgaat cccgccgtcg
     1441 atctgcagca catcgccaag atctacgccg gacaagaggt atccaagcgg ggcacctctc
     1501 tgcagatgag cggcccggcc ctctggttcc gcaacctcag ccgtgccctg cagttgaccg
     1561 ccagctcgtc gccgcccgtc caggcgctca gcgacgtatc gctgcggatc gcgcctgggg
     1621 agatctttgg tctggtgggg cgcaacggct ccggcaagac gacgttgatc aagatcatgg
     1681 ccggactgat ccgcccgacc agcggcgacg ggtcggtcgt ggggttctct ctacaagagc
     1741 cacaggagat ccgcaaacgc gtctcctacg tctcgacgac cggctggatg ggcttggagt
     1801 ggccgctcac cgccgaagac aatatgcgct actttgcgtt gctatgcggc atgccagatc
     1861 gcctggcgcg gcagcgaacc gagcaagcgt tgcgggacgt cgagctgtgg gaggagcgca
     1921 cgaagtaccc gtctcagctc tcgaatggca tgcgccagcg cgtgattctg gcacgggcgc
     1981 tcctcttcca caccccgctg gtgctcctcg acgagccgac cgtcggcctc gatccactga
     2041 ccacgcgctc cgttctggag ctcatccgcg gtcccttgcg tgagcgtggc cagaccatcg
     2101 tgctcaccga ccatcagtcg gctgagatgg aagcggtcgc cggccgcatc gcgatcctcc
     2161 gcgaaggccg gatctggcga cagggcacgc cacgcgagct cgtcgcgcgt ctcgcgggtc
     2221 ttaccgtcgt cgagatccgc accgaggaga tggacctgcc gtccggcccg ccacccgaga
     2281 tcgtggtcgc cgcccaaccg ctcgaacggc ccggcgccct cggcgaacgg gtctggcggg
     2341 tgcatgccca tcgttgcccg gacgcgctgg agcaggtggt cgactggatc gagcagccgg
     2401 ctgggcgcat cgtcttcctg gccgagagcg agccgaccct gcgcgatctg ctcgcgctgc
     2461 cggaggagtt ggtgcctggg gaagccgcgg ccagcgacgt cgacctggcg gcaggccgat
     2521 gagccactcg cggaaaccgg ctatcgcggg cgcacctccc ggtgtgccgg cgcgggccac
     2581 gggcgcggca ggccggcgca gcctctggcg ggaattcgtg gcgctgtggg ccttcgggct
     2641 gcgcgagtac cgcatctggc agtcctatcg cgtcaaccag gtgctctggc tgacgaacct
     2701 cttcgtcacc acctacctct tctttctgct cggcaagacg ctggccgggg acgcggcgag
     2761 cgtcatcccc gcggcctacg gcaccaacta catgtcgttc atcgtcgtcg gcgtcaccat
     2821 caacgtcttc atcaacacca acctggtcga cccctacatc cgcatccagc gggcctattt
     2881 tcagggcacg atggatctct tcctgttgag cccgatgtcg atctacactc cattgctggg
     2941 cttgatgagc aagtcggcca tcgatgacta cccgcgcctc ttctttacct tcggcttcgg
     3001 catgctcctc ttcggcgcga ccttcacctt ccagtatgtc tggctggcgc tcttcttcac
     3061 cgcgctcttt ctagcggccg gcttcggcat cgggctgctc agcgccagct ccttccacct
     3121 gctcgacatc aagaagggca ccgagccgat cggctttgtc gtgcaggggc tgctggcgac
     3181 gctggtggcg ggcacctact acccggtgac cgtgctgccg ctgccgctgc ggtggctcgc
     3241 ctgcctgatc ccccacacct atgccttcga cgccctgcgc cggctgctcg accccaacgc
     3301 ccagctgggg cagccggtcc tgccgctcca gcacgccctt cccggactct cgccgatcat
     3361 ggtcgacggc ctcgcgctgg ggctgctcgc ggtgacattg ctcccgctcg gcttctacct
     3421 ctacggtcgc gggatcgagc gggcgcgcgg caacgggacg ctgacccgat ggcagtgatc
     3481 gacgtgcatg ggctgaccaa gcgctaccgc caggggcgcg gcgagccggt cacggtgctg
     3541 cgcgacgtcg atctgcgcct ggaggagggg accgtgctcg ggctactcgg cccgaacggc
     3601 gccggcaaga cgaccttgat caagatcctg gccggcctgc tgcgaccgga cgccggcggc
     3661 ggcagcgtcc tcggcttcga cctggggcga cagcacgccg agatccgggc gcgggtgagc
     3721 ctggtcgcgc cgacggccga cgtcgggatc gacaacaatc tcaccgtccg ccagaatctc
     3781 accttctggg cgcccatcta cggcttgcgc ggtgccgccg cgcgcacccg catcgacgcc
     3841 ttgctcgcgc agctggagct gaccgacaag gccgacgcct ggcccatgca catcagcgcc
     3901 ggccaacgtc aacgtctcgc gctggctcgg tcactcatcg ccgagaaccg gctcgtcttt
     3961 ctcgacgagc caaccaacaa gctcgatctg gagggggttc gcttggtccg aaccatgatc
     4021 acccagctaa accgggagct cggcgtcacc atcgtgctga cgacccatgt gatggaggag
     4081 gcggaagaac tgtgcggtga gatcgcgttg ctccatcaag gagcgctcgt ggcgcacgcg
     4141 cccaccggac agctcacctg gtcgctggcg ctggcgcgac cggtcaccat cacgctgcgc
     4201 ggcacgaacc cgcccgaccc caactggctc gcgtgcctgc gcgccgtacc cggcgtgacc
     4261 aacgtgcatg cgcccacgcg cgacgccgcg ggtcgggtcg tcctggcggc cgaatcaact
     4321 gacctacggc tgaccacacc ggcgctgctg gcctacctgc gagccagcgg cgtcgccctg
     4381 gcgagcatgc gggcggagcc ggtcacgctg gatgatgtat tcagcgcgct ggggtccctc
     4441 taatggagat gccgctgccg gtgcaggcac gcgagcacca gcaaggtgga gcgctcgcct
     4501 tcctccgcgc gtgctgggcg tccggctggc tcgaataccg caacctgcgc tactacccca
     4561 ccaatctcgg tctggcggcc gtccaggagc tcacgaccgt cggggtttgg tacttcgtcg
     4621 cgcgcttcct gcattcggcg gctgatgcct cggtggcgca gtacggcggc aattacctgg
     4681 cgtttgtcgt gctcggcgtg ctgctgaatc aagtcgcgct ggcggcgctg accggtccat
     4741 tccagaccgt ctcggaggcc ttctgggaca agcgcctgga gacctaccgc ctgtcgatcc
     4801 acggtatctg ggccaacgta accgggcggc tcgcctggcg ggtcgctttc tcgagcgtcc
     4861 tccagttcgg cgccctggtc atcctgatcg ccatcggagc catccggatc catgccggta
     4921 tccacgtcga gctggcgctg ctggcgttgc tgctcctggt cgtcgcgaat gccggactcg
     4981 gcatcgccgg agccagcctc ttctttctgt tggaagtcaa gaacggcagc gacccgatca
     5041 cctgggccta caaatacctg gtcatgctgg ctgccggact ctatgtcccg gtcgtactcc
     5101 tgcccgtctg gctgcgggcc gccggggcag ccctgccgca aacctacgcc ttcgcggccg
     5161 agcggtcgat cgtcttgacc ggggcgggct ggtcgcaggg atccgtcggg catgatctta
     5221 tcggcctggc gctcggtgcg ctcgtggcgc tcggcgccgg ctgcgtcatg ttgaatctcg
     5281 ccctgcggcg ggccgaacgc cagggcggga tcggcgtcgt cgtctgacgc tcctgccggc
     5341 gctcgtttct ctgacaccgt gtcagcctgt ccatcagaga gaaggctg
//
LOCUS       DASUBI010000175         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_390222, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000175 DASUBI010000000
VERSION     DASUBI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390222"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..977
                     /locus_tag="VFU72_03445"
     CDS             189..977
                     /locus_tag="VFU72_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEU5422572.1"
                     /translation="MTAPRDPTAYPEVNAILRLLLEQTRAVLGTQFVGLCLYGSLSSG
                     DFDPASSDIDFLAVTEGELPPETVERLGAMHARLASSGLPWANRLEGSYIPRAALLRH
                     DPSNARHPTIGLDWDFGLGQHGPEWVIQRWIVREQGVTLAGPPPATLIDPVTPEMLRA
                     AVIDSLLGFWAEQLQGPEPEWLRTREYQAFAVLTMCRALYTLAQGALVSKPAAAAWAR
                     AALDSQWVPLIERALAWRHDRRPDDLTETLDFVRFAVERARQAQ"
     gene            1290..>3038
                     /locus_tag="VFU72_03450"
     CDS             1290..>3038
                     /locus_tag="VFU72_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5422573.1"
                     /translation="MQTTAAPAATESRQRVYVIFAGLMLVMLMASLDGTIVATALPTI
                     VGDLGGLAHLSWVVVGYLLAETIITPLYGKLGDLYGRKLLLQSAVVIFLLGSVLCGLS
                     RNLPELILFRTLQGIGGGGLSVTSTAVIGDVISPRERGRYQGIFGAVFAVATVIGPLL
                     GGFFVDHLSWRWIFYVNLPVGALALAVIAVALPPIAARAAHKIDYLGTILIALGLSGI
                     VLFTSLGGTTFAWSSPESIGFLALGVLCLIGFVFAERRAAEPVLPLSLFRNRVFTVTS
                     AIGLIVGLALFGSVTYLPVFLQVVQGASPTRSGLEMLPLMAGVLVTSVASGFLISRWG
                     RYKIFPIIGTAVTTVGLLLLARLGADSSRPATWLAMVVLGLGLGMVMQVLVIAVQNAV
                     DYRQLGVATSGATLFRLVGGSLGTAIFANRLSANLARFLPPSAGAQGASLAQGNPAAL
                     AALPPALRHDIILAYAHSLSPVFLVAAPVTAVAFVLTWFLREVPLRQTVEAGATNLEK
                     GFAMPQEPSSLDLIERGLGRIISREGAFQIYARIAARAGLPTSPQATWLLVRLGEASP
                     ISATALASRLRLAPSAL"
BASE COUNT          377 a         1130 c         1014 g          517 t
ORIGIN      
        1 atagcgacat cagcgccatt ctctggaacg acattatcgt cggcgtggtc gtcctggtgc
       61 tcagcgcctg gagcgcgctg gcacaggaga ccctggcgcg ctagctcccg gaccaccgcc
      121 agtgccggcg cccgtttccc cttgtcggac gccggtgcgg cggtccaggc ccgggtatcc
      181 ttgcgccgat gacggcgcca cgcgatccga cagcctaccc cgaggtcaac gccatactgc
      241 ggctgctgct ggagcagacg cgcgccgtgc tgggcacgca gttcgtcggc ctctgcctct
      301 acggctcgct ctcctccggc gacttcgacc ccgccagcag cgacatcgac ttcctggccg
      361 tcaccgaagg cgagctgcca ccggagaccg tcgaacggct gggcgcgatg cacgctcgcc
      421 tcgcctccag cggcctcccc tgggccaacc ggctcgaagg ttcttatatc ccccgtgccg
      481 cgctgctccg ccacgatccg tccaatgcgc gccacccgac catcggcctc gactgggact
      541 tcggcctggg tcagcacggg ccggagtggg tcatccagcg ctggatcgtg cgcgagcagg
      601 gcgtcaccct cgccggtccg cccccagcca cgctgatcga cccggtgacg cccgagatgc
      661 tgcgcgccgc cgtgatcgac agcctgctcg gcttctgggc ggagcagctt caggggcccg
      721 agccggagtg gctgcgcacg cgcgagtacc aggccttcgc cgtgctgacg atgtgccgcg
      781 cgctctacac cctcgcgcag ggcgcgctcg tctccaagcc ggccgctgcc gcctgggcgc
      841 gcgccgcgct ggactcccag tgggtgccgc tgatcgagcg cgccctcgcc tggcgccacg
      901 acaggcgccc cgacgacctg accgagactc tggacttcgt gcggttcgcg gtcgagcgtg
      961 cgcggcaggc gcagtaggcg cggtcggcgc ggacgccagc taccaggctg acgaacacgt
     1021 tgggtgccgc gtcagcacgg atccttcgac aagctcaaga ggacagggga ggttagcggc
     1081 cttcggacga ggcaattcgc cgtcgtgcgc cccgtactct ccctgtcatc ctgagcggag
     1141 tcgaaggatc cgttttgacg cgccactcat gctcctcgcg ggcggctacg ctgccggacg
     1201 cacgacacaa cccgtccaat ccccggcggc acgacgagtg cactggacct ctccggtcgg
     1261 ttgccaacca tccgacgaga ggagtgtcaa tgcagaccac cgccgcgccg gcggccaccg
     1321 agtcgcggca gcgggtgtac gtcatcttcg ccgggctgat gctggtgatg ctgatggcct
     1381 cgctcgacgg caccatcgtg gctacggcgc tgccgaccat cgtcggcgac ctcggcggcc
     1441 tggcgcacct ctcctgggtc gtggtcggct acctgctggc cgagacgatc atcaccccgc
     1501 tctacggcaa gctgggcgac ctctacgggc gcaaactgct cctgcagagc gcggtcgtca
     1561 tcttcctgct cggctcggtg ctctgcggcc tgagccggaa cctgcccgag ctgatcctct
     1621 tccgtacctt gcaggggatc ggcggaggcg ggctgagcgt cacctcgacc gccgtcatcg
     1681 gcgacgtgat ctcgccgcgc gagcgcgggc gctaccaggg catcttcggc gccgtcttcg
     1741 ccgtggcgac cgtgatcggc ccgctgctgg gcggcttctt cgtcgatcac ctctcctggc
     1801 gctggatctt ctacgtcaac ctgccggtcg gcgccctggc gctggcggtc atcgcggtgg
     1861 cgctgccgcc gatcgccgcg cgggccgcgc acaagatcga ctacctcggc accatcttga
     1921 tcgcgctcgg cctctccggc atcgtcctct tcaccagcct gggcggcacc accttcgcct
     1981 ggagctcgcc cgagagcatc ggctttctcg cgctgggagt gctctgcctg atcggcttcg
     2041 tcttcgccga gcgccgcgcc gccgagccgg tgctgccgct ctcgctcttc cgcaaccgcg
     2101 tcttcaccgt caccagcgcc atcggcctga tcgtcgggct ggcgctgttt ggctcggtca
     2161 cctatctgcc ggtcttcctg caggtggtgc agggcgccag cccgacccgc tcagggctgg
     2221 agatgctgcc gctgatggcc ggcgtgctgg tgacctcggt cgccagcggc ttcctgatca
     2281 gccgctgggg gcgctacaag atcttcccga tcatcggcac ggcggttacg accgtcgggc
     2341 tgctgctcct cgcgcgcctg ggggcggaca gcagccgccc cgccacctgg ctggcgatgg
     2401 tcgtgctcgg gctggggctg ggcatggtga tgcaggtgct ggtgatcgcg gtgcagaacg
     2461 cggtcgatta tcgccagctg ggagtggcga cctcgggcgc gacgctcttc cggttggtcg
     2521 gcggctcgct cggcaccgcc atcttcgcca atcgcctgag cgccaacctg gccagattct
     2581 taccgccctc ggccggcgcg cagggcgcca gtctggcgca ggggaacccg gcggcgctgg
     2641 ctgcgctgcc gcccgcgctg cgccacgaca tcatcctggc ctacgcgcac tctctgagcc
     2701 ccgttttcct ggtcgccgcg ccggtcaccg ccgtggcctt cgtcctcacc tggttcctgc
     2761 gcgaggtgcc gctgcggcag acggtcgagg caggcgcgac caacctggag aagggtttcg
     2821 ccatgccgca ggagccgtcg tcgctcgacc tgatcgagcg cgggctgggc cggatcatct
     2881 cgcgcgaggg cgccttccag atctacgccc gcatcgcggc gcgcgccggc ctgcccacct
     2941 cgccgcaggc gacctggctg ctggtgcgcc tcggcgaggc gtctccgatc tcggccaccg
     3001 cgctggcgtc gcgcctgcgg ctggcgccaa gcgcgctg
//
LOCUS       DASUBI010000176         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1044670, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000176 DASUBI010000000
VERSION     DASUBI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044670"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFU72_03455"
     CDS             <1..1015
                     /locus_tag="VFU72_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5422574.1"
                     /translation="GVDTSTIAASARVESAPSFNAQVSLPGSEHWENFAVNGVSADFV
                     TNSDAPLQVRATGYADDQAVWNAVKSGQNLAVIDSTALPANGGFGTPRFQLPGLKAGA
                     KTMEPTTVEFRNPATGKSETVTIIGVLDSKVSMFTGLFVMDSTFVKVFPEPSSITYYV
                     KVKPGVDAKAEARTIEAKLIAYGVQAQSIKEAVEQASSMSQGFLYLIEGFMGLGLLVG
                     IAALGVISFRAVVERRQQIGMLRAIGYQRAMVSASFLIESSMITLLGVGSGVILGLLL
                     AYQLMSSPDVTGSTGSETSFVIPWLLIAAFVVVSVGASLLMAYIPSRQASRVPIAEAL
                     RYE"
     gene            complement(1121..1288)
                     /locus_tag="VFU72_03460"
     CDS             complement(1121..1288)
                     /locus_tag="VFU72_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5422575.1"
                     /translation="MLARSRVALKFMTYGILLGVLFAPRSGSQTRHELMGWAKNNLGG
                     VMSGLGLGSRG"
     gene            complement(1525..1914)
                     /locus_tag="VFU72_03465"
     CDS             complement(1525..1914)
                     /locus_tag="VFU72_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5422576.1"
                     /translation="MSRQNGIGKHPGTLIFGSIVGGAAGTIATLLLTPWSGQRLREQL
                     FGPVAGAVSPAVEKAGSVAQSVTAKGSSLAAPAVDTVTSVVQKGTEKGSAVVFGATTQ
                     LQNLRGGAEEPPPPTPEASNGKPLTTL"
     gene            2104..3240
                     /gene="sucC"
                     /locus_tag="VFU72_03470"
     CDS             2104..3240
                     /gene="sucC"
                     /locus_tag="VFU72_03470"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5422577.1"
                     /translation="MNIHEYQAAEILAGYGVPVNAGIVCTTPEEVGAAAAQLGTPVVI
                     KAQVHTGGRGKAGGVKVARTPEQARELAGQILGLDIRGHVVHKVLVAPAVDIQREIYL
                     GVVLDRTARGVTVMASAAGGVDIEEVAATTPEQIHRVHVSPYLGLFPYQARALAFDLG
                     IEPKLVNGFAGVALKLFQAYRDSDATLAEINPLVLTGEGAWQALDSKIVLDNNALYRH
                     PVFEDLRDMNEEVATEIEARNAGISFVKLDGSIGCIVNGAGLAMATMDAIKLHGGEPA
                     NFLDVGGGASAEQVAKAFQLVLTDPAVRSVLVNIFGGITRGDLVAEGLIEALHELKVQ
                     VPIVVRLVGTNAEEGRRILQEAGITALDAMDEAAAAAVAAARGA"
BASE COUNT          543 a         1121 c         1149 g          539 t
ORIGIN      
        1 cggcgtcgac accagcacga tcgcggcgag cgcgcgcgtc gagtcggcgc cgagcttcaa
       61 cgcacaggtc agcctgccgg gcagcgaaca ctgggagaat ttcgcggtca acggcgtgag
      121 cgcggacttc gtgaccaact ccgacgcgcc gctgcaggtg cgtgcgaccg gctacgccga
      181 cgaccaggcc gtctggaacg cggtcaagtc cggtcagaac ctggcggtga tcgactccac
      241 ggcgctgccc gccaatggtg gcttcggcac gccgcgcttc cagcttccgg gcctcaaggc
      301 gggcgccaag acgatggagc cgaccacggt cgagttccgc aacccggcga ccggcaagag
      361 cgagacggtg accatcatcg gcgtgctcga ctcgaaggtc tccatgttca ccggcctctt
      421 cgtgatggac tcgaccttcg tcaaggtctt ccccgagccg agctccatta cctactacgt
      481 caaggtcaag cccggcgtcg acgccaaggc ggaggcgcgg acgatcgagg ccaagctgat
      541 cgcctacggc gtgcaggcgc aatcgatcaa ggaggcggtg gagcaggcgt cgagcatgtc
      601 gcagggcttc ctctacctga tcgagggctt catgggcctc ggcctgctgg tcggcatcgc
      661 cgcgctgggg gtgatctcct tccgggccgt cgtcgagcgg cggcagcaga tcggcatgct
      721 gcgggccatc ggctaccagc gggcgatggt ctccgccagt ttcctgatcg agtcgtcgat
      781 gatcacgctg ctgggcgtcg gctcgggtgt gatcctcggg ctgctgctgg cctatcagct
      841 catgagcagc ccggacgtca ccggcagcac cggctcggag accagcttcg tgatcccctg
      901 gctgctgatc gcggccttcg tggtggtgtc ggtgggcgcg tcgctcctga tggcctacat
      961 cccgtcgcgc caggcctcgc gcgtcccgat cgccgaggcg ctgcgctacg agtgaggcgc
     1021 cctcaccccc agccccactg ccaacgaggt gggagagggg agcacgaagg atttgtaggg
     1081 gcgggtttcg aacccgccct tgcttgttgc gcgtatgccc ctacccgcgg ctgccaaggc
     1141 cgaggccgct catcaccccg ccgaggttgt tcttggccca gcccatcaat tcgtggcgcg
     1201 tctgcgatcc gctacggggc gcgaagagga cgccgagcag aattccgtag gtcatgaatt
     1261 tgagcgctac gcgactccgt gccagcatgg ttccctcccg gttgattcat cgtgcgacgg
     1321 cgactgcccg tcctggggaa tcggtggctt gtgaggacgg atccttcgac aagctcagga
     1381 tgacaggggt agtaaggggc ggacgacaac gattcgtgtc atccgatagg ccacaaagca
     1441 aacactgtca tcctgagctt gtcgaaggat ccgtgcaaca ctcgcacccc aacgccatcg
     1501 ctcgccacca ttgctcgtct gcgcctacag cgtcgtcagc ggcttgccgt tggacgcctc
     1561 cggcgtgggg ggcggaggct cttccgcgcc gccgcgcagg ttctggagct gggtggtcgc
     1621 cccgaagacg acggcgctcc ccttctccgt gcccttctgc accaccgaag tgaccgtgtc
     1681 gaccgccggc gcggccagcg aagacccctt cgcggtcacg ctctgcgcca cgctgccggc
     1741 cttctcaacg gccgggctga cggcgccggc caccgggccg aagagctgct cgcgcagccg
     1801 ctggcccgac cagggcgtga gaagcagggt cgcgatcgtt ccagccgcac caccaacgat
     1861 gctcccgaag atcagcgttc ccggatgctt gccgatgcca ttctgcctgc tcatcacaac
     1921 cctcccgctt atccggccca ccgaatccgc atcaggctca tgctatcaaa ggttgtgcca
     1981 gaggtcttca gcacggctgc ggcacctgcg ctgggccgga gcgtgctatg atcgacgatt
     2041 ggatcgtcgc ggatcgcgat agacccggga ccttgaaccg cggtgcagca gtagaggtgg
     2101 cgcatgaaca ttcacgagta ccaggcggcg gagatactgg cggggtacgg ggttccggtc
     2161 aacgccggga tcgtttgcac cacgcccgag gaggtaggcg ccgcggcggc gcagctcggc
     2221 acgccggtcg tgatcaaggc gcaggtgcac accggcgggc gcggcaaggc gggcggtgtc
     2281 aaagtggcgc gcacgccgga gcaggcgcgc gagctagccg gccagatcct ggggctcgat
     2341 atccgcgggc acgtggtcca caaggtgctg gtcgcgcccg ctgtcgatat ccagcgcgag
     2401 atctacctcg gcgtggtgct cgaccgcacg gcgcgcggtg tcacggtgat ggccagcgcg
     2461 gcgggcggag tcgacatcga ggaggtcgcg gccaccacgc ccgagcagat ccaccgcgtg
     2521 catgtcagcc cctacctcgg cctctttccc taccaggcgc gcgcgctggc cttcgacctc
     2581 ggcatcgagc cgaagctggt caacggtttc gccggcgtgg cgctgaaact cttccaggcc
     2641 taccgcgact ccgacgccac cctggccgag atcaacccgc tggtgctgac cggcgagggc
     2701 gcctggcagg cgcttgactc caagatcgtg ctcgacaaca acgcgctcta ccgccacccg
     2761 gtcttcgagg acctgcgcga catgaacgag gaggtcgcca ccgagatcga ggcgcgcaac
     2821 gccggcatca gcttcgtcaa gctcgacggc tcgatcggct gcatcgtcaa cggcgccggg
     2881 ctggcgatgg cgaccatgga cgcgatcaag ctccacggcg gcgagccggc caacttcctc
     2941 gatgtcggcg gcggcgccag cgccgagcag gtcgccaagg ccttccagct cgtgctgacc
     3001 gacccggcgg tgcgctcggt gctggtcaac atcttcggcg gcatcacacg cggcgacctg
     3061 gtggccgagg ggttgatcga ggcgctgcac gagctgaagg tgcaggtgcc gatcgtggtg
     3121 cggctggtgg gcaccaacgc ggaggagggc cggcgcatct tgcaggaggc cggcatcacc
     3181 gcgctggatg cgatggacga ggccgcggcg gcggcggtgg ccgcggcgcg cggcgcctag
     3241 cggcgggagg agagagcgat ggcagttcta gtcaatgacg atacgcgcct cctcgtccag
     3301 ggcatcactg gccgcgaggg ttcgttccac acggggcaga tggtggagta cg
//
LOCUS       DASUBI010000177         9792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_212921, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000177 DASUBI010000000
VERSION     DASUBI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212921"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFU72_03475"
     CDS             complement(<1..182)
                     /locus_tag="VFU72_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422578.1"
                     /translation="MRRSALLLTLLLLSLVLPGAVFVPTAGAAEAVRVVSQQASANFP
                     TSMSFALTADTEQPVTA"
     gene            complement(172..1279)
                     /gene="prfB"
                     /locus_tag="VFU72_03480"
     CDS             complement(join(172..1209,1211..1279))
                     /gene="prfB"
                     /locus_tag="VFU72_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871204.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5422579.1"
                     /translation="MIENIEQTVASIAERLDRIGVRLDLPAKQQEIQDLELESSNPDF
                     WSDAQRAQRLMRHLGALKQQVDSWHALRRQASDLEELLVLAEADEALRAEIAAETEQA
                     LGQLEQLELRLLLSGPYDEADAIVAVHAGTGGVDAQDWAEMLVRMYLRWAAQAGLQAE
                     VLDEAEGEEAGIKSATIELRGENAYGYAKAEAGTHRLVRLSPFDAAHRRHTAFALVEV
                     LPEVEDDTDVQINDDDLRIDTYRSSGAGGQHVNKTSSAVRITHIPTGVVVTCQNERSQ
                     MQNKETAMKILRARLIERKLREREEEQARLKGEHVNPGWGNRIRSYVLHPYTLVTDHR
                     TEHSTGDIQAVLDGEIGPFVEAYLQQQLGAAHAS"
     gene            complement(1370..2170)
                     /locus_tag="VFU72_03485"
     CDS             complement(1370..2170)
                     /locus_tag="VFU72_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5422580.1"
                     /translation="MQATVRLGRIFGIPIGIHFSWLLVFALVTITLGYGIFPDWLGGS
                     LVVAWLAALAASLLFFVSLIAHELGHALVARRYGIPVHSITLFALGGVAGLEREPERA
                     GAEFWMALAGPAVSLALALLNGGLWYLTQHGPDAPNAVLFYLALANGMLVLFNLIPGY
                     PLDGGRLLHAAIWGLTGNLRRATRAAALIGTLVGLGFIAFGLFELIFETIYSGIWLVA
                     IGWFLQQLARDAYRSARPPRPSLPSFPAVERVRPRLPLAPPTIEQPEK"
     gene            complement(2460..3077)
                     /locus_tag="VFU72_03490"
     CDS             complement(2460..3077)
                     /locus_tag="VFU72_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264297359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3033..3035),aa:Sec)
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU5422581.1"
                     /translation="MAETTNIKVWYDYTUPYVYTAAVMLHHAKEQLGDQLNIDWKYFS
                     LEQVNSQHGPDWKLWEQPESYPSRARLAWKGAIAARQQGEQAFDRFNLALLTARHADG
                     KELTEPQTIVEAAKQAGLDMDRFKQDFEQATLAPVGRDFEEGREEYGVFGTPTIVFED
                     GRAGYMRLRPLPPEDQLLDVWQQVKSVINGRPSILEIKRPVPPAK"
     gene            complement(3172..3777)
                     /gene="coaE"
                     /locus_tag="VFU72_03495"
     CDS             complement(3172..3777)
                     /gene="coaE"
                     /locus_tag="VFU72_03495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476513.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5422582.1"
                     /translation="MTRTYVIGLTGNIACGKTLVLDELRALGAETIDADRVAHQVMQP
                     GGPAYQAIVAHFGPEIVAPDGALDRRKLGAIVFNSPAELDALERIVHPATVAAIRAQV
                     ARATAPVVVIDAIKLFEAGLDQDSDQIWAVRCAPAQQLERLMRRNNFSRAEALARINA
                     QPPQEEKLARADRVIDNSGSVDDTLAQVRAAWATIPHAGAV"
     gene            complement(3774..5723)
                     /locus_tag="VFU72_03500"
     CDS             complement(3774..5723)
                     /locus_tag="VFU72_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5422583.1"
                     /translation="MPTTTILSVDNLTKSFGTELIFKDVSFQLQERERVALVGVNGAG
                     KSTLLAILAGLERADSGEVVPRAGLRLAHQAQEARFDPGKSVREAALDAFAEARQAGA
                     ELTELEQRMGGAEGAELDALFERYSELSARFEAAGGYDIDHRTDQVLMGLGFGEEDFA
                     TPVAQLSGGQRTRLALAHALLTDPDLLLLDEPTNHLDLAALDWLEGFLRGWNRAFLVI
                     SHDRFFLDRVTNRTLDLSFGTLEDYPAGYSGYLRLREERLARRLAEYEAQQEFIARTE
                     EFIRKYKAGQRSKEARGRATRLARLERVERPQEQEALRLRLAGGLRSGRSVLSSKEIT
                     AGYPPRRGEVEPTVLVRTPALLIERGDRVGLIGPNGAGKTTLLRTIMGELKPLKGRLQ
                     YGVNVKPAYYAQGHEGLDPNATALSTILDGHPMSEEGARSLLGRFHFSGDDAFKPVSA
                     LSGGERSRLALARLTLEQANFLILDEPTNHLDISSREALEEVLEEYEGTILFVSHDRY
                     LIDRIANRLWVVEDGELKTYLGNFSDYQRTRAAAAPAAAKATATPAPEPAPESRTGEP
                     RASERERRQLQQELSAAERRVGKLEQRLNELNDDLALATIEQDVARITRLGEEYQAAQ
                     AELEAAYARWEELGSRLDELLEPSR"
     gene            6039..7364
                     /gene="lysA"
                     /locus_tag="VFU72_03505"
     CDS             6039..7364
                     /gene="lysA"
                     /locus_tag="VFU72_03505"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5422584.1"
                     /translation="MLWPITAVRDPQGELTIGGVRVSELAAEFGTPLYLYDAATIRAR
                     CRAYRDAFAAAYPRSRVVYAGKAYLGAAILEIIAEEGLWLDVVSGGELVFAVRAGFPP
                     ERIVLHGNNKSAEELALARRLGIGEIVVDNHHEIDLLETLARELPGAVDVLLRVNPGI
                     DAHTHEYRKTGIVDSKFGLLLAPGDAARAVERLLAIPGVRLRGYHAHVGSQINELEPF
                     VAAVDVLFGFAAEMRARFGVVPEQISPGGGLGIQYLAGESEPDIPGYAEQVAGAVARA
                     AERTGFPAPLLAVEPGRSIIGPAGVATYRVGAIKEIPGIRRYVCVDGGMGDNIRPALY
                     GAEYTAELADRAGGAGARVTIAGKYCESGDVLIGEVILPEPRPGDLVVIPAAGAYCLA
                     MASNYNLALRPAVVLVGDEAGAARLIQRRETYDDLLTREVGLVGARGGS"
     gene            7455..9059
                     /gene="rpoD"
                     /locus_tag="VFU72_03510"
     CDS             7455..9059
                     /gene="rpoD"
                     /locus_tag="VFU72_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU5422585.1"
                     /translation="MAELMRRHPDGAEDEFDLVDGAADELSETPTIDTAAIERLRAVG
                     QSRGYVTVAEIVQATGASDTSAAIVEAIRTQLEADSVMVTEQDDTSELAAIPDEPSVI
                     DIFSIRRGEVDLTGVSLDDPVRLYLREIGAVALLSWDDEVKLAKAMECGDYLTRLQQE
                     LTGADRVSPSSAAIALAVYRNFKQGFPIAVAFYAESFPGRRAATKSQVLADVLPFADE
                     REPAVVTTCERLGLSREALEEELRRRRIEHDLLPPEVQERIAELESWLPDDEARIVFD
                     CEDEALARRWNEMLDRGDYARQKLTEANLRLVVSVAKKYVGRGMSMLDLVQEGNLGLI
                     RAVEKFQYHKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINRLIRTSRQLH
                     QELGREPTSEEVAVRMEMPPDKVRDIMKISQEPVSLEMPIGEEEDSNLGDFIEDSKTL
                     APSDAASLKLLKEQVGAVLGTLSERERRVLELRYGLDDDGRSRTLEEVGREFGVTRER
                     IRQIEAKALRKLRHPNRSKKLRDFLE"
     gene            9191..9481
                     /locus_tag="VFU72_03515"
     CDS             9191..9481
                     /locus_tag="VFU72_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422586.1"
                     /translation="MAAGGATAGAAAAAAQYNALKATGAIIEVDPRTLLDILNKGDRP
                     LVVVNHGGFWTKRYNYLTPYRGLIFAAKSPTPLQLPSRAEIVAAQKFWLPRV"
     gene            9554..>9792
                     /locus_tag="VFU72_03520"
     CDS             9554..>9792
                     /locus_tag="VFU72_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5422587.1"
                     /translation="MFYDHARIQVTGGNGGNGAVSFRREKYVPHGGPDGGDGGRGGGV
                     YLRVDPHLNTLIAFKYQQRFHAGNGSHGMGARRTG"
BASE COUNT         1478 a         3287 c         3487 g         1540 t
ORIGIN      
        1 gcggtcactg gctgctcggt gtccgcggtc agcgcgaagg acatgctggt gggaaaattg
       61 gcgctcgcct gctggctgac gacacgtact gcctcggccg cacccgccgt gggcacgaag
      121 accgcgcccg gcagcaccag gctcaggagc agcagggtca gcagcagagc gctacgacgc
      181 atgggcggcg ccgagctgct gctgcaggta ggcctcgacg aaggggccga tctcgccgtc
      241 gagcacggcc tggatatcgc cggtactgtg ctcggtgcgg tggtcggtga ccagcgtgta
      301 ggggtgcagt acgtaggagc ggatgcggtt gccccagccg gggttgacgt gctcaccctt
      361 gagccgggcc tgctcctcct cgcgctcgcg cagcttgcgc tcgatcaggc gggcgcgcag
      421 gatcttcatg gccgtctctt tgttttgcat ctgcgagcgc tcgttctgac aggtcacgac
      481 gacgccggtg gggatgtggg tgatgcgcac cgccgagctg gtcttgttca cgtgctggcc
      541 gccggcgccg gaggagcggt aggtgtcgat gcgcaggtcg tcgtcgttga tctgcacatc
      601 ggtgtcgtcc tcgacctccg gcagcacctc gaccagggcg aaggcggtat ggcggcggtg
      661 ggcggcgtcg aagggtgaga ggcgcaccaa ccggtgggtg ccggcctcgg ccttggcgta
      721 gccgtaggcg ttctcgccgc gcagctcgat ggtcgcgctc ttgatgccgg cttcctcgcc
      781 ctcggcctcg tcgaggacct cggcctggag cccggcctgc gccgcccagc gcagatacat
      841 gcggaccagc atctcggccc aatcctgggc atcgacccca ccagtgccgg cgtggacggc
      901 gacgatggcg tcggcctcgt cgtagggacc gctgagaagc aagcgcagct ccagttgctc
      961 gagctgcccc agcgcctgct ccgtctccgc ggcgatctcg gcccgtaggg cctcatccgc
     1021 ctcggccagt acgagcagct cttccaggtc ggacgcctgg cgccgcaggg cgtgccagct
     1081 atcgacctgc tgcttgagcg cgccgaggtg gcgcatcagc cgctgggcgc gctgggcgtc
     1141 gctccagaag tcggggttgc tcgactcgag ctcaaggtct tgaatctcct gctgtttggc
     1201 aggcaagtca aagacgcacc ccgatccggt cgagacgctc ggcgatgctg gcgacggtct
     1261 gttcgatgtt ctcgatcatt gggaatgtct ccgtagtgaa cggccggcgc gcgtgcgcgg
     1321 ccggccgtcc ctgctcctgg tttacccgcc aagtatagca cgcaccggct cacttctcgg
     1381 gctgctcaat ggtcggcggc gccagcggca gacgcggccg gacgcgctcg accgccggaa
     1441 agctcgggag gctcgggcgg ggcgggcggg ccgaacggta ggcatcgcgg gccaactgct
     1501 gcaggaacca gccgatcgcc accagccaga tgccggagta gatcgtctcg aagatcagct
     1561 cgaagaggcc gaaggcgatg aagcccagcc cgaccagcgt gccgatcagc gcggcggcac
     1621 gagtggcgcg gcgcaggttg ccggtcagtc cccagatggc ggcatgcagc agccggccgc
     1681 cgtcgagtgg gtagccgggg atcaagttga agagcacgag catgccgttg gccagcgcca
     1741 ggtagaaaag gacggcgttg ggcgcgtccg gcccgtgctg ggtgaggtac cacagcccgc
     1801 cattgagcag cgccagcgcc aggctaacgg ctggcccggc cagggccatc cagaactcgg
     1861 ccccggcgcg ctccggctcg cgctccagcc cggccacccc gccgagggcg aagagggtta
     1921 tgctgtgcac gggaatcccg tagcgccggg cgaccaacgc gtgccccagc tcgtgggcga
     1981 tcagcgagac gaagaagagc aggctggccg ccagcgccgc cagccaggcg acaaccagtg
     2041 aaccgcccag ccagtccggg aagatgccgt agccgagcgt gatcgtgacc agcgcgaaga
     2101 ccagcagcca gctgaagtga atgccgatgg ggattccaaa gatccggccg agccgaaccg
     2161 tcgcctgcat gcgccgcgcc tgctttcgcc gtgattccgg cgccggttcg cgcgacgccg
     2221 cctagagact gattgtacgc tacgcaggga acgggccagg cgggggcata cgacagtgca
     2281 gggtggcacg tgaggacgga tccttcgact gcgctcagga tgacagggac aagggagagg
     2341 ctgacgacga cgatctgcgt cgtccggtag gctgctcccc tcccctgtca acctgagctt
     2401 gtcgaaggat ccgtcgtgga gcaaagctca aggtcgtcta tgggcgctgc gccgccgccc
     2461 tacttggccg gcggcaccgg gcgcttgatc tcaagaatgc tcgggcggcc gttgatgacc
     2521 gacttgacct gctgccagac gtcgagcagt tgatcctccg gcggcagcgg gcggagccgc
     2581 atgtagccgg cgcggccgtc ctcgaagacg atggtgggcg tgccgaagac gccgtactcc
     2641 tcgcggccct cctcgaagtc tcgtccgacg ggggccagcg tcgcctgctc gaagtcctgc
     2701 ttgaaccggt ccatatccag cccggcctgc ttggcggctt cgacgatcgt ctggggctcg
     2761 gtcagttcct tgccatccgc gtggcgcgcg gtcaagagcg ccaggttgaa gcggtcgaag
     2821 gcctgctcgc cctgctggcg cgccgcgatg gcgcccttcc aggcgaggcg cgcccggctg
     2881 ggatagctct cgggctgctc ccacagcttc cagtccggcc cgtgctggct attcacctgc
     2941 tccagtgaga agtatttcca gtcgatgttg agctgatcgc cgagctgctc tttggcgtgg
     3001 tgcagcatga ccgctgcggt gtagacgtat ggtcaggtgt agtcgtacca gactttgatg
     3061 ttggtcgtct cggccacgag gcgctcctct cgcgtgcggt ccactccggt ggggtggcgg
     3121 tggccaccgt gctcctttgt agcatagctt ggcgcgtggt ggatgcccgg gtcagacggc
     3181 gccagcgtgt gggatggtgg cccaggccgc gcggacctgg gccagcgtgt cgtcgacgct
     3241 gccgctgttg tcgatcacgc ggtcggcgcg cgccagcttc tcctcctggg gcggctgggc
     3301 gttgatccgg gccagcgcct ccgcgcggct gaagttgttg cggcgcatca ggcgctcgag
     3361 ctgctgcgcg ggcgcgcagc gcactgccca gatctggtca ctgtcctggt ccaacccggc
     3421 ctcgaagagc ttgatggcgt cgatgacgac caccggcgcg gtcgcgcggg cgacctgggc
     3481 gcggatggcc gccaccgtgg ccgggtgaac gatgcgctcc agcgcgtcca gctccgcggg
     3541 gctattgaag acgatcgcgc ccagcttgcg ccggtcgagc gcgccgtcgg gggcgacgat
     3601 ctccgggccg aagtgggcca cgatcgcctg gtaggccgga ccgcccggct gcatcacctg
     3661 gtgcgccacg cggtccgcgt cgatcgtctc ggcgccgagc gcgcgcaact cgtcgagcac
     3721 cagcgttttg ccgcaggcga tgttgccggt caggccgatg acgtaggtgc gcgtcaccgg
     3781 ctcggctcca ggagctcgtc gagccggctg cccagctcct cccagcgcgc gtaggccgcc
     3841 tcaagctcgg cctgtgccgc ctggtactcc tcgcccagcc gcgtgatgcg cgcgacatcc
     3901 tgctcgatcg tcgccagcgc caggtcgtcg ttcagctcgt tgagccgctg ctccagcttg
     3961 ccgacccggc gctcggccgc gctcagctcc tgctgcagtt ggcgccgctc gcgctcgctg
     4021 gcgcgcggct cgcccgtcct cgactcgggc gccggctccg gcgcgggggt ggctgtcgcc
     4081 ttggcagccg ccggggcggc cgccgcgcgc gtgcgctggt agtcgctgaa gttgcccagg
     4141 taggtcttca gctcgccgtc ctcgaccacc cagagccggt tggcgatacg gtcgatgaga
     4201 tagcggtcgt gggagacgaa gaggatggtt ccctcgtact cctccagcac ctcctccagc
     4261 gcctcgcgtg agctgatgtc gaggtggttg gtcggctcgt cgaggatcag gaagttggcc
     4321 tgctccagcg tcagcctggc cagcgccagg cggctgcgct cgccgccgga gagcgccgag
     4381 acgggtttga acgcgtcgtc gccgctgaag tggaaccggc ccagcagcga gcgcgcgccc
     4441 tcctcgctca ttgggtgccc gtcgaggata gtcgagagcg cggtcgcgtt gggatcgagt
     4501 ccctcgtggc cctgggcgta gtaggcgggc ttgacgttga cgccgtactg caggcgcccc
     4561 ttgagcggct tcagctcacc catgatcgtg cgcagcagcg tcgtcttgcc ggcgccattc
     4621 gggccgatca agccgacgcg gtcgccgcgc tcgatcaaga gcgcgggcgt gcgcaccagc
     4681 accgtcggct cgacctcgcc tcgccgggga gggtaaccgg cggtgatctc cttgctgctg
     4741 agcacgctgc ggccgctgcg cagacccccg gccagccgca ggcgcagcgc ctcctgctcc
     4801 tgtgggcgct cgacccgctc cagccgcgcc agccgggtag cgcgaccgcg cgcctccttg
     4861 gagcgctgcc ccgccttgta cttgcggatg aactcctcgg tgcgcgcgat gaactcctgc
     4921 tgcgcctcgt actcggccag ccgccgcgcc aggcgctcct cgcgcaggcg caggtagccg
     4981 gagtagcccg ccgggtagtc ctccagcgtg ccgaaactca ggtcgagggt gcggttggtc
     5041 acccggtcga ggaagaagcg gtcgtgcgag atgaccagga aggcgcggtt ccagccgcgc
     5101 aggaacccct ccagccagtc cagcgccgcc aggtcgagat ggttggtcgg ctcgtcgagc
     5161 aggagcagat cgggatcggt cagcagtgcg tgcgccagcg cgagtcgcgt gcgctgtccg
     5221 ccggagagct gcgcgaccgg ggtggcgaag tcctcctcgc cgaagccgag gcccatcagt
     5281 acctgatcgg tccggtggtc gatgtcgtag ccgcccgccg cctcgaagcg cgcgctcagc
     5341 tcggagtagc gctcgaagag ggcgtccagc tcggcgccct cggctccgcc catgcgctgc
     5401 tccagctcgg tcagctcggc gccggcctgg cgcgcctcgg cgaaggcgtc cagtgccgcc
     5461 tcgcgcaccg atttgccggg gtcgaatcgc gcctcctgcg cctggtgggc cagccgcagg
     5521 ccggcgcgcg gcacgacctc accgctgtcg gctcgctcca gcccggccag gatggccagc
     5581 agcgtactct tgccggcgcc attgacgccg accaacgcca cgcgctcgcg ctcctggagc
     5641 tgaaagctga cgtccttgaa gatcagctca gtgccgaagc tcttggtcaa attgtccacg
     5701 gagaggatcg tcgtcgtcgg catggtttcg tccctctgca tatcgccagc acaccgatat
     5761 cacgcggcag accgtcgtct gccccgagtc actgtgtgcg ggtgagggac gcacgccggc
     5821 cgcgagtggt ggttgcccac ccgtgccacc ggcacccatc gggcaggtcg ctacgccggt
     5881 cacccccggc atagtaccca actcctaaat cgggccgatg tctcgcattc atacaccaag
     5941 tttatccgtt ttgggagcgg cgcgccactg cgcgaccagc cggcttgcgg tggcggggta
     6001 taatcgggcc gcgattcagt gcgacacagg gagacgtgat gctctggccg atcacagccg
     6061 ttcgtgaccc ccagggagag ctgaccatcg gcggcgttcg ggtgagcgag ctggccgccg
     6121 agttcggcac gccgctctat ctctacgacg cggctacgat ccgggcgcgc tgtcgtgcct
     6181 atcgagacgc cttcgccgcg gcctacccgc gcagccgtgt ggtctatgcc ggcaaggcct
     6241 acctgggagc agcgatcctc gagatcatcg ccgaggaagg gctctggctc gacgtcgtct
     6301 ccgggggtga gctggtcttc gcggtgcgcg ccggcttccc gcccgagcgc atcgtgctcc
     6361 atggcaacaa caagagcgcg gaggagctgg cactggcccg gcgactgggg atcggcgaga
     6421 tcgtggtcga caaccaccac gagattgacc tgctggaaac cctggcgcgg gaattgcctg
     6481 gagcggtcga cgtgctcctg cgggtcaacc ccggcatcga cgcccacacg cacgagtatc
     6541 gcaagaccgg catcgtcgac tcgaagttcg gcctgctgct cgcgcccggc gacgcggcgc
     6601 gggcggtcga gcggctgctg gcgatccccg gcgtgcggct gcgcggctat cacgcgcacg
     6661 tcggctcgca gatcaacgag ctcgagccgt tcgtggccgc ggtggacgtg ctcttcggct
     6721 tcgcggccga gatgcgcgca cgcttcggcg tggtcccaga gcagatcagc cccggcggcg
     6781 ggctgggcat ccagtacctg gccggcgagt cggagccgga tatcccgggc tacgccgagc
     6841 aggtggccgg cgcggtggcg cgggcggcgg agcgcacggg gttcccagcg ccgctgctgg
     6901 cggtcgagcc ggggcgctcg atcatcggtc cggcgggcgt cgccacctac cgggtcggcg
     6961 cgatcaagga gattcccggc atccggcgct acgtctgcgt cgatggtggg atgggcgaca
     7021 acatccgccc ggcgctctac ggcgccgagt acacggcgga gctggcggat cgggcgggcg
     7081 gcgcaggggc gcgcgtcacg atcgccggca agtactgcga gtcgggcgac gtgctgatcg
     7141 gggaggtaat cctgccggag ccgcgccccg gtgatctggt ggtaatcccg gcggccggcg
     7201 cctactgcct ggcgatggcc agcaactata atctggccct tcgcccggcg gtggtgctgg
     7261 ttggggatga ggcaggcgcg gcgcggctga tccagcggcg cgagacgtac gatgatctcc
     7321 tgactcggga agtcgggcta gtgggagcac gcggcggcag ctagtggcgc atttgctttt
     7381 cgacgcgcga cgtagtaaga ctggcggacc gcagccagga agaactacgc gagcgacgcg
     7441 ggggagtatc ggtcgtggct gagttgatgc gacgtcaccc ggacggggcc gaggacgaat
     7501 tcgaccttgt cgacggggcc gccgatgagc tgtccgagac gccgacgatc gacacggccg
     7561 cgatcgagcg gctgcgcgcc gtgggccaga gccgcggcta cgtcaccgtc gcggagatcg
     7621 tgcaggcgac cggcgccagc gacaccagtg ccgccatcgt cgaggcgata cgaacccagc
     7681 tcgaggccga cagcgtgatg gtgacggagc aggacgacac cagcgagctc gccgccatcc
     7741 cggacgagcc gagtgtcatc gacatcttct ccattcgccg cggcgaggtc gatctaaccg
     7801 gcgtcagcct ggacgatccg gtgcgtctct acctgcgcga gatcggcgcg gtggcgctcc
     7861 tgagctggga cgatgaggtc aaactggcga aggcgatgga gtgcggcgac tacctcacgc
     7921 ggctgcagca ggagctgacc ggcgccgatc gtgtgtcgcc ctccagcgcg gccatcgcgc
     7981 tggccgtcta ccgcaacttc aagcagggct tccccatagc cgtcgccttc tatgccgaat
     8041 cgttccccgg ccgccgggcg gccaccaagt cccaggtgct agccgatgtg ctccccttcg
     8101 cggacgagcg cgagccggcg gtggtgacga cctgcgagcg gctcggcctg agccgcgagg
     8161 cgctcgaaga ggagctgcgc cgccgccgca tcgagcacga cctactgccg cccgaggtgc
     8221 aggagcggat cgccgagctg gagagctggc tcccggacga cgaggcgcgg atcgtcttcg
     8281 actgcgagga cgaggcgctg gcccggcgct ggaacgagat gctcgaccgg ggcgactacg
     8341 cgcggcagaa gctgaccgag gccaacctgc gcctggtcgt cagcgtggcc aagaaatacg
     8401 tcggccgcgg catgtcgatg ctcgacctgg tgcaggaggg caacctgggt ctgatccggg
     8461 cggtcgagaa gttccagtac cacaagggct acaagttctc gacctacgcc acctggtgga
     8521 ttcgccaggc gatcacgcgc gccatcgccg accaggcgcg cacgatccgc atcccggtcc
     8581 acatggtgga gacgatcaac cggctgatcc gcacctcacg ccagttgcac caggagctgg
     8641 ggcgcgagcc gacctccgag gaggtcgccg tgcgcatgga gatgccgccg gataaagtgc
     8701 gcgacatcat gaagatctcg caggagccgg tttcgttgga gatgccgatc ggcgaggaag
     8761 aggacagcaa cctcggggac ttcatcgagg actcgaagac gctggcgccc tccgacgccg
     8821 cctcgctcaa gctgctcaag gagcaggtgg gcgcggtgct cggcaccctc tccgagcgcg
     8881 agcggcgcgt gctggagctg cgctacgggc tcgacgacga cggacgcagc cgcacgctgg
     8941 aggaggtcgg gcgcgagttc ggcgttaccc gcgagcgtat ccggcagatc gaggccaagg
     9001 ccctgcgcaa gctgcgccac cccaaccgca gcaagaaact gcgcgatttc ctggagtagc
     9061 ccgcccgcga tcatccccgc tccggtccgc cacgtgcgat tgcgtccaag agtggcgacc
     9121 ggaggctgac gcgcgctctt gatcgttgtt ggagtgagcc tcgcgcaagc gggggagttg
     9181 gagacagcac gtggcagcgg gaggggcaac ggccggggcg gcagccgccg cggcgcagta
     9241 caacgcgctc aaggcgacgg gtgccatcat cgaagtcgac ccgcggacgc tgttggatat
     9301 cctcaacaag ggcgatcggc cgctcgtcgt cgtcaaccat ggcggcttct ggacaaagcg
     9361 ctacaactac ctcactccct accggggact gatcttcgcc gccaagtcgc ccacgccgct
     9421 gcagctgccg agccgggccg agatcgtggc ggcgcagaag ttctggctgc cgagggtctg
     9481 aggctctgaa cctgctggcc ccggcacggc ggttcacgat catgctccgg ggcagggtag
     9541 gacggaaggt gtcatgttct acgaccacgc acgcatccag gttaccgggg gcaacggcgg
     9601 caacggcgcc gtcagcttcc ggcgcgagaa gtacgtgccg cacggcggac cagacggcgg
     9661 cgacggcggc cgggggggcg gcgtctacct gcgcgtcgat ccgcacctca acacgctgat
     9721 cgccttcaag taccagcagc gctttcacgc cggcaacggc agccacggca tgggcgccag
     9781 gcgcaccggg ca
//
LOCUS       DASUBI010000178         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1072129, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000178 DASUBI010000000
VERSION     DASUBI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072129"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFU72_03525"
     CDS             complement(<1..267)
                     /locus_tag="VFU72_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422588.1"
                     /translation="MSPDDGPFARIARRIDPGARLLRRWELTGGVSAHVTALELERAS
                     GETTRLVVRQYGPADLLRNPNSAADEFRLLRLARAAGVAAPQPCL"
     gene            complement(324..794)
                     /locus_tag="VFU72_03530"
     CDS             complement(324..794)
                     /locus_tag="VFU72_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEU5422589.1"
                     /translation="MSAEPIDLGDDVTRGAVAGVVAAGAMAVAMWLDLAVTGAKTDEL
                     RLLGGLVPGLRRLWPITGLAMHLANGAALGAFYGWVQAGLPGKPWQRGLLLAEVENIA
                     LWPVLLVLARLQPASRGGTAQHFNTPGAFAVEVLRHAVYGVVLGLVFDRLIPRR"
     gene            complement(791..1525)
                     /locus_tag="VFU72_03535"
     CDS             complement(791..1525)
                     /locus_tag="VFU72_03535"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU5422590.1"
                     /translation="MALADEIAAWLRQEIDRVGASGAAVGLSGGIDSAVVAGLAARGL
                     GPERVQALIMPIHSQQQDVEDAQLVADAFGIPSQTIDLGPVFDLLRSLLPQGTQLAEA
                     NLKPRLRMLTLYFVANSRNLVVVGTGNRSELMVGYFTKYGDGGVDLLPLGSLLKHDVY
                     EVARGIGVPQRIIDRPPSAGLWAGQTDETEMGISYADLDQILTALTQGDTTGLAPEKL
                     AKVQRMIAGSEHKRHTPPIFQPGERP"
     gene            complement(1614..2105)
                     /locus_tag="VFU72_03540"
     CDS             complement(1614..2105)
                     /locus_tag="VFU72_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5422591.1"
                     /translation="MSDPAPAFAHSHPGFNYRVAGVCLHAGHVLLHRAEDDDFWALPG
                     GRPEFGEPSDAALVREMREEIEVEVTIERFLWLLENFYEYRGQQKHELCCYYVMAMPP
                     GSPLLDVSREFSGYEPTVRLIYRWFPLAALDAVTLYPTFLKAGLRALPAHPARVVQVD
                     END"
     gene            complement(2098..2718)
                     /locus_tag="VFU72_03545"
     CDS             complement(2098..2718)
                     /locus_tag="VFU72_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422592.1"
                     /translation="MPAEFDPAYAARLLARQQALTREASQVIERLQLRELLARAGEPF
                     QIGSSRYGLMVWRDLDFSILCAGSAAEAIASVIAALVANPLVRRLDYLDETGPRSPSG
                     LPGDQRHYVVLRAEPIAGQEWKIDLSFWIAEGQRGELEHPSHMAERLTEETRLRILHL
                     KDRWHRLPGYPDIVSASDIYDAVLEHGVRTPDELARYCEERGLPSE"
     gene            3090..>3352
                     /locus_tag="VFU72_03550"
     CDS             3090..>3352
                     /locus_tag="VFU72_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU5422593.1"
                     /translation="MERIATTELVAHAGERVRLAGWLERLRQLGSIDFLILRDGVGSA
                     QVVIDSPELREQLAGLPAESVLAVEGPAVRAPQAPGGVELRAER"
BASE COUNT          500 a         1165 c         1201 g          486 t
ORIGIN      
        1 caagcatggt tgcggcgcgg cgacccccgc cgcgcgcgcc aggcgcagca gccggaactc
       61 gtcggcggca ctgttcgggt tgcggagcag gtcggccggg ccgtactggc gcaccaccag
      121 gcgggtcgtc tcgccgctgg cccgctcaag ctccagcgcc gtgacgtgcg ccgagacgcc
      181 gccggtcagc tcccagcggc gcaatagccg cgcgcccgga tcgatgcgcc gggcgatgcg
      241 cgcgaacggc ccgtcgtcag gagacacggt tggtctggac cctccgggat gcggccgacc
      301 cactcgatag ccactttcgc ctgctaccgt cgcgggatca gccggtcgaa gacgaggccg
      361 aggacgacgc cgtagacggc gtggcgcaga acctcgacgg cgaaggcgcc gggcgtattg
      421 aagtgttggg ccgtgccgcc gcggctggcg ggctgaaggc gcgccagcac cagcaggacc
      481 ggccagaggg cgatgttctc gacctcggcc aagagcagcc cgcgctgcca gggcttgccc
      541 ggcagcccgg cctggaccca gccgtagaag gcgcccagcg cggcgccgtt ggccaggtgc
      601 atggcgagcc cggtgatcgg ccagaggcgg cgcagccccg gtaccagccc gccgagcagc
      661 cgcagttcgt cggtcttggc gccggttacg gccaggtcga gccacatcgc gaccgccatc
      721 gctccggcgg cgaccacgcc cgccaccgcg ccgcgggtga cgtcgtcccc cagatcgatc
      781 ggttcggcgc tcatggccgc tcccccggct ggaagatcgg cggcgtgtgc cgcttgtgct
      841 cggagccggc gatcatgcgc tgcaccttcg cgagcttctc tggcgccaga ccggtcgtat
      901 cgccctgtgt cagcgccgtc aggatctgat cgaggtcggc gtagctgatc cccatctcgg
      961 tctcgtcggt ctgccctgcc cagagcccgg ccgagggtgg acggtcgatg atccgctgcg
     1021 gcacgccgat gccgcgcgcc acctcgtaga catcgtgctt cagcaggctg cccagcggca
     1081 ggagatcgac accgccgtcg ccgtacttgg tgaagtagcc gaccatcagc tcgctgcggt
     1141 tgccggtgcc gacgaccacc aggttgcggc tgttggccac gaagtagagg gtcagcatgc
     1201 gcaggcgcgg cttgaggttg gcctcggcca gttgcgtgcc ctgaggcagg agcgagcgca
     1261 gcagatcgaa gaccgggccg aggtcgatgg tctgggacgg gatgccgaag gcatcggcca
     1321 ccagttgcgc gtcctccacg tcctgttgct ggctgtggat cggcatgatc agcgcctgga
     1381 cgcgctccgg gcccagcccg cgcgccgcca gcccggccac gacggccgag tcgatgccgc
     1441 cggagagccc gacggccgcg ccacttgccc caactcggtc gatctcctgg cgaagccagg
     1501 cggcgatctc atccgccagc gccatcgtcc cctcccgccg cgcccagtcc agcatgcgag
     1561 cacgcgcacg gcccgatgat gcggcgcgcc accgcgcccg tcaagcggag cgctcaatcg
     1621 ttctcgtcga cctggacgac gcgcgcgggg tgcgccggca gcgcgcgcag acctgccttc
     1681 aagaaggttg ggtagagcgt gaccgcgtcg agcgccgcca gtggaaacca gcggtagatc
     1741 agccgcacgg tcggctcgta gccgctgaac tcgcgcgaga catccaggag gggcgagccg
     1801 ggcggcatcg ccatcacgta gtagcagcac agctcgtgct tctgctggcc ccgatactcg
     1861 tagaaattct ccagcagcca gaggaagcgc tcgatggtga cctcgacctc gatctcctcg
     1921 cgcatctcgc gcacgagggc cgcgtcggag ggctcgccga actcgggccg cccaccaggc
     1981 agcgcccaga agtcgtcgtc ttcggcgcgg tgcaagagga cgtggcccgc gtgcaggcag
     2041 accccggcca cgcggtagtt gaatcccggg tggctgtgcg cgaatgccgg cgccggatca
     2101 ctcactcggc agcccccgct cttcgcaata gcgcgcgagc tcatccggcg tgcgtacgcc
     2161 gtgctccagg accgcatcgt agatgtcgct ggccgagacg atgtcgggat aaccagggag
     2221 ccggtgccag cgatctttga ggtgcaggat cctcaggcgc gtctcttccg tgagacgctc
     2281 agccatgtgc gaggggtgct ctagttcgcc ccgctggccc tcggcgatcc agaaggagag
     2341 atcgatcttc cactcctgcc cggcgattgg ctccgcacgg agcacgacgt agtggcgctg
     2401 gtcgccgggg aggccgctgg ggctgcgggg gccggtctcg tccaggtagt cgaggcgacg
     2461 caccagcggg ttggccacca aggcggcgat caccgacgcg atcgcctccg cggcggaccc
     2521 cgcacagagg atgctgaagt cgagatcgcg ccagaccatg agcccgtagc gcgagctgcc
     2581 gatctggaac ggctcaccgg ctcgcgccag cagctcgcgc agttgcagac gctcgatcac
     2641 ctggctcgct tcccgcgtca gcgcctgctg ccgcgcaagc agccgcgcgg cgtaggccgg
     2701 gtcgaactcc gctggcatcg gccgtccctt tcgtgggctg atgcgcttct ctgctgaccg
     2761 gcgccatagt ctaacagacg tcgctcaggt tgcggcggga gcgagaagcg agtaggctct
     2821 ggggaacgcg acgatggaga cgagtagcgt ggccaaccgg agtgcagcga gccgtcgctg
     2881 gtgggaagac ggcctccgga ccgcgcgaag accctccgga gcgcgaggaa gaacggatca
     2941 atctcagtaa gcctcgccgg ttcgcccccg ttagcgggcg tcgaggctgc cgcgttctcg
     3001 acggcagcga agtgcggtgg caccacgggt ggcccccctc gtccgcacgg gcgagggggg
     3061 ccagtgtttt cctcacggga ggtgtcaccg tggagcgtat cgccaccacg gagcttgtcg
     3121 cgcacgccgg tgagcgtgtg cggctggccg gctggctgga gcgtctacgc cagctcggct
     3181 cgatcgattt cctcatcctg cgcgacggcg tcggcagcgc ccaggtcgtg atcgacagtc
     3241 cggagctgcg cgagcagttg gccgggctgc cggccgagtc ggtgctggcg gtcgaggggc
     3301 cggcggtgcg cgcgccccag gcgccgggcg gcgtcgagct gcgcgccgag cg
//
LOCUS       DASUBI010000179         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_50011, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000179 DASUBI010000000
VERSION     DASUBI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50011"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFU72_03555"
     CDS             <1..293
                     /locus_tag="VFU72_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5422594.1"
                     /translation="KFGVPVCPHAGGVGLCELVQHLAMFDYVAVSASTTDRVIEYVDH
                     LHEHFENPVVVRDGRYLPPEAPGYSVTMRPASLAEFDFPLGPAWALPAEQQR"
     gene            475..675
                     /locus_tag="VFU72_03560"
     CDS             475..675
                     /locus_tag="VFU72_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422595.1"
                     /translation="MANEANQRARQNPPSNDTGISQERRAALEPALRALLRDFQRLEE
                     LVTPTTEPAPTPELEGYADERR"
     gene            662..2080
                     /locus_tag="VFU72_03565"
     CDS             662..2080
                     /locus_tag="VFU72_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5422596.1"
                     /translation="MSAVELCRLSLAEAAGLVARREVSPVALTEAALARIEQLNPILN
                     AFITVTGETALAEARAAEREIAGGAYRGPLHGIPLSLKDLYCTKGVRTTGGSRLLGGF
                     VPDEDATATRKLRQAGAVLLGKTAMLELAYGEEHPDYGATRNPWDIEYGTNGSSSGSA
                     AAVAAGLGYGSLGSDTGGSIRLPAAWCGIVGLKPTYGLVSRAGVLPLSWSLDHAGPMT
                     RTVRDCALLLQAIAGHDPADPTSARRPVPDYSAGLDRVPSDLTVGVFAPAPDDRVLPE
                     VRAALDAAAQTLRNAGISVREVRQPYPDQATRALLAMLYAEASSYHLPWLRERPQDYS
                     ANTRERLELGARLPATTYLSALRARRVIIAAYQELMRDVDILLTPPAPTASYRLDGPS
                     TAPVGPEGDRMAPLVRFSGPFDLTGLPAIAVPAGLTGAGLPIGVQLVARPFAEPTLLQ
                     TAQVVEQALRDRLPRPDTGLVV"
     gene            complement(2266..3000)
                     /locus_tag="VFU72_03570"
     CDS             complement(2266..3000)
                     /locus_tag="VFU72_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEU5422597.1"
                     /translation="MQERIFTRIERPDPQIVSGLARLGVSTVYEAMGLSLGARQLLEP
                     AFQVLVADQQIAGPAITAQCSAGDNTMSHVVGVLAQPGDVLVVAGGDPQTALWGDLTS
                     LVAHVHRVAGVVVDGGVRDVRVIRELGLPVWARVVSPARPAKGGPGSANVPVRAGGVL
                     IHPGDIVVADDDGVVVVPRASATEILARAEAREAREITLRSELIAGHHRALYEALGIE
                     KLLEAAGVETVDDSFDHWRERNGGRG"
     gene            3115..3597
                     /locus_tag="VFU72_03575"
     CDS             3115..3597
                     /locus_tag="VFU72_03575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5422598.1"
                     /translation="MIEPDEFRELMARFPSGVTVVTTAGSDGYHGVTVSAFCSLSLDP
                     PLVLVCIDQAIQSHDLITNAPAFVVNLLAREQTFVAEQFAGRTPLADPTFKRVPHRLG
                     QLGAPVLDGSLGFVECEHWAAYDGGDHTIVVGLARALGLGPASDPLVYFERQFTDLAW
                     "
     gene            complement(3791..4288)
                     /locus_tag="VFU72_03580"
     CDS             complement(3791..4288)
                     /locus_tag="VFU72_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422599.1"
                     /translation="MSTDAEVTLRCELRPGDLGRLTELHGRLYAEEYGFDPRFEAYVA
                     ESLGELVQRGRPERDRLWLAELSGRLVGSVGILGRENGAAQLRWFLVHPDARRRGLGS
                     RLLREAVEFARAAGSRSLYLWTVSPLADAARLYIAAGFRLTEETPAAAPWGVMLREQR
                     YDLEL"
     gene            complement(4311..4604)
                     /locus_tag="VFU72_03585"
     CDS             complement(4311..4604)
                     /locus_tag="VFU72_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5422600.1"
                     /translation="MSALWAVLGMGAGVYALRLSGLALRDVALPPVWERALGFVPIAL
                     LTALVLVTLDVGASGGWERLLAAGVAGLIAQRTGRMWTCILGGMVCLWLFRLV"
     gene            complement(4601..5329)
                     /locus_tag="VFU72_03590"
     CDS             complement(4601..5329)
                     /locus_tag="VFU72_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU5422601.1"
                     /translation="MRGAVRPRSTRRDGERTAWLTLFRRGFLAMAPLWAGAIPVGVAY
                     AVAARDAGLGAGATQLMSLLVFSAAAQVSAVSLLRAGASLPLLIATAMALNVQLLLIG
                     LAVGRQARPSWRQRLLTASVLTDGAYGVSAALGPLRLPLLLGAGSSMYLAWNGGTFLG
                     LLVGAALPDPERLGLGFIVPLAFLAVLVPLVRTRTSGLVVLSAAAATLLLAPHVPTGV
                     AVLGAGLAGSAAGGWWSRRGEDAA"
     gene            5396..6280
                     /locus_tag="VFU72_03595"
     CDS             5396..6280
                     /locus_tag="VFU72_03595"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422602.1"
                     /translation="MSSSSKSNSRRRGTRGRDAPTPGGERLRAVRERAGRTQLWVEAE
                     AELGTGYLQRIESGRVRLPVRATLERILGALGARYSERREVLELFGYAVATPLPDEDD
                     LAWARDASRQELADFPFPAYVLDCTHRLIAWNRTVPHLFGLRPDGPELARLARHSIQA
                     AWFDTASPLARLVAEPDSFLPALIRAMRYEMRPFRDEAWYADLLDQLLVLPRFRHYWA
                     LVEEEEPPVTETRAVMPLRLDVPGAGSLLFRLSAERFALDARFRVIYYFPSDEATMRR
                     CVAWAAHEAAGAPWPVRR"
     gene            6346..7467
                     /locus_tag="VFU72_03600"
     CDS             6346..7467
                     /locus_tag="VFU72_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEU5422603.1"
                     /translation="MLLDVALAFLVSRLTFYAAALVARWAIPEYAAGAYQPWHTSSPA
                     LIDASWRWDAGWYGTIIAHGYRLVPGQNNVAFFPLYPLLVRLVLLPFSLKWMYLAAVL
                     VNHLTFLAALLLIWNYVERIGGRALARRTLYLLAIFPGSFFFAAAYSESVFLLTAAGV
                     FLALQRERYGLAGGCALLASLARPPGILLALPIAVVLWRRHRARLAELARHGTALLLV
                     PLGPLFLMTYFWWRFDRPLAFLSAQGAWSRTHATPWWSLAKSLSLITAPGTWNLHLMV
                     VINTAAVLLALVLGLALVRREPAAAVFVLASITVPLVFAASTNPSTSLARFVAVLFPI
                     FLPLARWARHRLTLALLTAVFLPTNFLMAALFVRWYWVV"
     gene            7589..8890
                     /locus_tag="VFU72_03605"
     CDS             7589..8890
                     /locus_tag="VFU72_03605"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422604.1"
                     /translation="MAEFDGANEGSGLRGNPLSSLRYRDNRVFWIGTSFSSIGQAAFL
                     VASSWLAFRLGGSSAVGIVTFATMIPLFLATLAGGVLADRQERRRLVLASEAAQGIFA
                     LVIAFQALRGAMSFAQLVVLVFLTGVARAIEMPTVQTVLPSLVPRDELLNIFSLNNLA
                     NRGSRFVGPALVATILELRGPAPAFLVIALLYLLAMSQVVRVPRMHQAEVSQLNLREQ
                     VAEGVRYIAGHSPLGLLLLVIVLHCLLTMSFDSTLPLFASQNLKGNGAIYSTMVSAMG
                     LGSIMAALLLASIHSQRGRGALLLIGGIGSGAATALMAISMNSLLALSAIFLVGVMTT
                     WFMTLANTMMQEAVPDGLRGRISGIYLMSASGVMSFGNLGAGYLAAQYGSAAVLGLPA
                     LGFIVLLLAFSVLRPALRRLYRAGSFSLDQAGAMEPVSAGD"
BASE COUNT         1258 a         3375 c         3064 g         1375 t
ORIGIN      
        1 ccaagttcgg tgttccggtg tgcccgcacg cgggcggggt cgggctgtgc gagctcgtgc
       61 agcacctggc catgttcgac tacgtcgcgg tcagcgccag caccaccgac cgggtgatcg
      121 agtacgtcga ccacctgcac gagcacttcg agaacccggt ggtggtgcgc gacggccgct
      181 atctgccgcc cgaggccccc ggctacagcg tcaccatgcg cccggcctcg ctggccgagt
      241 tcgactttcc gctcggcccc gcctgggcgc ttcccgcgga gcaacagcgg tagcaggcaa
      301 tgaggagtac ggggagatcc ttcgccgcgg cctcagagcc tgccctgatg agcttgtcga
      361 tgggatgact ggggggagta tgccttttct ctgtcatcct gaggccgcag ccgacggatc
      421 tctcatcggc aacacgcgat agcaaccgaa cacggggagg gaggaccgcc taccatggcg
      481 aacgaagcga accaacgagc acggcagaat cccccgagca acgacactgg gatctcccag
      541 gagcgccgcg ccgccctgga gccggcgctg cgggcgctgc tgcgcgactt ccagcgcctg
      601 gaggagctgg tgacgccgac caccgagccg gccccgacgc cggagctgga gggctacgcc
      661 gatgagcgcc gttgagctct gccggctgtc cctggccgag gccgccgggc tggtcgcgcg
      721 gcgcgaggtc tcgccggtcg cactgaccga ggcggcgctg gcgcgcatcg aacagctcaa
      781 cccgatcctc aacgccttca tcaccgttac cggcgaaacc gctctcgctg aggcgcgggc
      841 ggccgagcgc gagatcgcgg gcggagccta ccgcggcccg ctgcacggca tcccgctctc
      901 gctgaaggat ctgtactgca ccaaaggcgt gcgcaccacc ggcggctcgc ggctccttgg
      961 cggcttcgtg ccggacgagg acgccacggc cacgcgcaag ctgcgccagg ccggcgccgt
     1021 gctgctcggc aagaccgcca tgcttgagct ggcctacggc gaggagcacc ccgactacgg
     1081 cgccacccgc aatccctggg acatcgagta cggcaccaac ggctccagca gcggctcggc
     1141 cgcggcggtg gcggccgggc tcggctacgg ctcgctcggc tcggacaccg gcggctcgat
     1201 ccgcctgccg gcggcgtggt gcggcatcgt cgggctgaag ccgacctacg ggctggtcag
     1261 ccgcgcgggc gtgctcccgc tctcctggtc gctcgaccac gccggcccga tgacccgcac
     1321 cgtgcgcgac tgcgctctgc tcttgcaggc catcgccggc cacgaccccg ctgaccccac
     1381 ctccgcccgg cgcccggtgc ccgactacag tgccgggctc gaccgggttc cgagtgatct
     1441 gacggtcggc gtcttcgcgc ccgcgccgga cgaccgcgtg ctccccgagg tgcgcgcggc
     1501 gctcgacgcc gccgcccaga cgctgcgcaa cgcggggatc agcgtgcgtg aggtgcgcca
     1561 accctacccc gatcaagcca cccgcgcctt gctggcgatg ctctatgccg aggccagcag
     1621 ctaccacctg ccctggctgc gggagcgtcc gcaggactac agcgccaaca cgcgtgagcg
     1681 gctggagctg ggcgcgcggc tccccgccac cacctacctg agcgcgctgc gcgcccgccg
     1741 cgtgatcatc gcggcctatc aggagctgat gcgggatgtc gacatcctgc tgacgccccc
     1801 ggcgccgacc gcctcctacc ggctcgacgg gccgtcaacc gcgccggtcg gtcctgaggg
     1861 cgaccgcatg gcgccgctgg tccgcttctc gggtccgttc gacctgaccg ggctgccggc
     1921 catcgcggtc ccggccgggc tgaccggcgc ggggctgccg atcggcgtgc agctcgtcgc
     1981 gcgccccttc gccgagccga ccctcttaca gacagcgcag gtcgtcgagc aggccctgcg
     2041 ggaccgtctg ccccggccgg atacgggctt ggtggtctga ggcgaacgac gaaaattagg
     2101 cggcgtgtga ggacggatcc tgcgacaagc tcaggatggc agggtaagag aggagcggac
     2161 gaggaagact atcctcgtcc ggcaggcccc tccctaacgc tgtcatcctg agcttgtcgc
     2221 aggatccgtc ctcacgcgct acagaaccct cgcgacggcc ccactctacc cccgcccccc
     2281 gttcctctcg cgccagtggt cgaacgagtc gtcgactgtc tcgacgccgg ccgcctccag
     2341 cagcttctcg atgcccaggg cctcgtagag cgcgcggtgg tggccggcga tcagctccga
     2401 gcgcagcgtg atctcgcgcg cctcgcgcgc ctcggcccgc gccagaatct cggtcgccga
     2461 cgcccgcggc accaccacca caccgtcgtc gtcggccacc acgatgtcgc ccggatggat
     2521 cagcacaccg ccggcccgca ccggcacgtt ggccgagccg gggccgccct tagccggtcg
     2581 cgccgggctg acgacgcgcg cccagaccgg cagtcccagc tcgcggatga cccgcacgtc
     2641 gcggacgcct ccgtcgacca ccacgcccgc cacccgatgc acgtgagcca ccagcgaggt
     2701 caggtcgccc cagagggcgg tctggggatc gcctccggcc acgaccagca cgtcgcctgg
     2761 ctgagccagc accccgacga cgtggctcat ggtgttgtcg cccgcgctgc actgcgccgt
     2821 gatcgcgggt ccggcgatct gctggtcggc caccagcacc tggaaggccg gctccagcaa
     2881 ctggcgcgcg cccagcgaca gccccatcgc ctcgtagacc gtcgagacac ccagccgcgc
     2941 caggccggac acgatctggg ggtccggccg ctcgatccgg gtgaagatcc gctcctgcat
     3001 atccttgctc ctccgcctcg ccgcacactg gccctgaacg gtaccagccg gagggcggaa
     3061 gttgaagcgg ggtgcccgcg tatactctca accaggggcg aggaaggacg acgcatgatc
     3121 gagccagacg agttccgcga gctgatggcc cgcttcccca gcggcgtgac ggtggtcacc
     3181 accgccggat ccgacggcta ccacggcgtc accgtctccg ccttctgctc gctctcgctc
     3241 gacccgccgc tggtgctggt ctgcatcgac caggcgatcc agagccacga cctgattacc
     3301 aacgcaccgg ccttcgtggt gaacctgctg gcgcgcgagc agaccttcgt ggccgagcag
     3361 ttcgccgggc gcacgccgct ggccgacccg accttcaagc gcgtgccgca ccggttgggg
     3421 cagctcggcg cgccggtcct cgacggctcg ctgggcttcg tcgagtgcga acactgggcg
     3481 gcctacgacg gcggcgacca caccatcgtc gtgggactgg cacgcgcgct cggcctcggc
     3541 ccggccagcg acccgctggt ctatttcgag cggcagttca ccgatttggc gtggtgacgt
     3601 ggcctccgac ggcgtgggat gcctggccag cacgggtcct tcgactgcgc tgaggatgac
     3661 aggggtagta gggggcggac gaggacgatt agcgtagtcg tgcgccccat cccctatcct
     3721 tgtcatcctg agcttgtcga aggatccgtg ctccatgcgg cacccatcgt cctcgacagg
     3781 ccgcccgcgc tcacaactcc aggtcgtacc gctgctccct gagcatgacg ccccagggcg
     3841 cggccgccgg ggtctcttcg gtcaggcgga agccggcggc gatgtagagc cgggcggcgt
     3901 cggccagcgg gctcaccgtc cacaggtaga gggagcggct gccggccgcg cgagcgaact
     3961 ccaccgcctc gcgcagcagc cggctgccca ggccgcgccg gcgcgcgtcg gggtgcacca
     4021 ggaaccagcg tagctgcgcc gcgccgttct ctcgcccgag aatcccgacc gaaccgacca
     4081 gccggcccga gagctcggcc agccagaggc ggtcgcgctc cggccgcccg cgttggacca
     4141 gctcgcccag gctctcggcc acgtaggcct cgaagcgcgg gtcgaagccg tactcctcgg
     4201 cgtagagccg gccgtgcagc tcggtcaggc ggccgagatc gcctgggcgc agctcgcagc
     4261 gcagcgtcac ctccgcatcg gtgctcatgg tcgatccggg ctcctctcac tcaaactagc
     4321 cgaaacagcc agagacagac catgccgccg aggatacagg tccacatccg ccccgtgcgc
     4381 tgggcgatga ggcccgcgac tcccgccgcc agcagccgct cccagccgcc gctcgcgccc
     4441 acatcgagcg tgaccagcac caacgcggtc agcagggcga tcgggacgaa gccgagcgcg
     4501 cgctcccaga cgggcggcag tgccacgtcg cgcagcgcca gccccgagag acgcagtgcg
     4561 taaacgccgg cccccatccc cagaacggcc cacagcgcgc tcatgccgcg tcctcgccgc
     4621 gccgcgacca ccaaccgccg gcggcgctgc cggccagccc ggcgccaagc accgccaccc
     4681 cagttggaac atgcggcgcc aacagcagcg tggccgcagc cgcgctgagc accaccagtc
     4741 cgctcgtgcg cgtgcggacc agcggcacca gcaccgcgag gaaggccagc ggcacgatga
     4801 agccgagccc cagccgctcg gggtcgggca gcgccgcgcc aaccagcagc ccgaggaatg
     4861 tgccgccgtt ccaggccagg tacatgctgc tgccggcgcc gagcagcagc ggtaaccgca
     4921 gcggccccag cgcggcgctg acgccgtagg cgccgtcggt cagcacgctg gccgtcagca
     4981 gccgctggcg ccaggagggg cgcgcctgcc gccccacggc cagcccgatc aggaggagct
     5041 ggacgttgag cgccatggcg gtcgcgatca gcagcgggag ggacgcaccc gcgcgcagca
     5101 gcgacaccgc gctcacctgc gccgccgccg agaagacgag caggctcatc aactgcgtgg
     5161 cgcccgcgcc cagcccggcg tcgcgcgccg ccacggcgta ggccacgccg accgggatgg
     5221 ctccggccca cagcggcgcc atcgccagga agccgcggcg gaagagcgtc agccaggccg
     5281 tacgttcgcc gtctcgtctt gtcgatctcg gccgcaccgc cccgcgcatc gctgccctgc
     5341 caccgtgccg aagtgctgat tccgctagct gctatgctag gcagcgggag gcggcatgtc
     5401 atcgtcatcg aagtcgaatt cgcgccggcg cggcacgcgc gggagagacg cgccgacgcc
     5461 ggggggcgag cggctgcggg cggtgcgcga gcgcgccggg cgcacgcagc tctgggtgga
     5521 ggccgaggcc gagctgggca ccggctacct gcagcggatc gagtcaggca gggtgcggct
     5581 gcccgtgcgc gctacgctgg agcgcatcct gggtgcgctg ggggcgcgct acagcgagcg
     5641 gcgtgaggtg ctggagctct tcggctacgc ggtcgccacg ccgctgccgg acgaggacga
     5701 tctcgcctgg gcgcgcgacg cctcccggca ggagctggca gacttcccct tcccagccta
     5761 cgtgctcgac tgcacccacc gcctgatcgc ctggaatcgt acggtgccgc acctcttcgg
     5821 gctgcggccg gatggcccgg agctggcgcg cctggcgcgg cactcgattc aggccgcctg
     5881 gttcgacacc gcctcgccgc tggcacggct ggtggccgag cccgactcct ttttgcccgc
     5941 gctgatccgt gccatgcgct acgagatgcg gcccttccgc gacgaggcct ggtacgccga
     6001 cctgctggat caactgctgg tcttgccgcg ctttcgccac tactgggcgc tggtggagga
     6061 ggaagagccg ccggtgaccg agacgcgcgc ggtgatgccg ctgcgcctgg acgtgcccgg
     6121 ggccggctcg ctcctcttcc ggctctcggc cgagcgcttc gcgctcgacg cgcgcttccg
     6181 cgtgatctac tacttcccct cggacgaggc gactatgcgc cggtgcgtgg cgtgggccgc
     6241 ccacgaggcc gctggagcac cgtggccggt ccggcgctga gaaggcggct ccgcgacgag
     6301 cggcgcgggg agcacctccg gtgttgggcc gtcgcccggc agcccatgct gctcgacgtg
     6361 gccctggcct tcctggtctc gcgcctcacc ttctacgccg ccgctctggt cgcgcgctgg
     6421 gccattccgg agtacgccgc cggcgcctac caaccctggc acacgtcctc ccccgccctg
     6481 atcgacgcca gttggcgctg ggatgccggc tggtacggca cgatcatcgc gcacggctac
     6541 cgcctggtgc ccgggcagaa caacgtcgcc ttcttcccgc tctacccgct gctggtgcgg
     6601 ctggtcctcc tccccttctc gctcaagtgg atgtacctgg cggcggtgct ggtcaaccac
     6661 ctcacctttc tggccgcgct gctcctcatt tggaattatg tcgagcgaat cggcgggcgc
     6721 gcgctggcgc ggcgcaccct ctacctgctg gcgatcttcc ccggcagctt cttcttcgcg
     6781 gcggcctaca gcgagtcggt cttcctcttg accgcggccg gcgtcttcct ggcgctccag
     6841 cgcgagcgct acgggctggc cggcggttgt gcgctgctcg ccagcctggc gcgcccgccg
     6901 ggcatcctgc tcgccctgcc gatcgcggtc gtgctgtggc ggcggcatcg cgccaggctg
     6961 gcggaactgg cgcggcacgg cacggcgctg ctgctggttc cgctcgggcc gctcttcctg
     7021 atgacctact tctggtggcg cttcgaccgg ccgctcgcct tcctcagcgc ccagggcgcc
     7081 tggagccgca cccacgccac accctggtgg tcgctggcga agtccctcag cctgatcacg
     7141 gcccccggaa cctggaacct gcatctcatg gtggtcatca ataccgccgc cgtcctgctc
     7201 gcgctggtgc tcgggctcgc gctcgtgcgg cgcgaaccgg ccgcggctgt cttcgtgctg
     7261 gccagcatca cggtgccgct ggtcttcgcc gccagtacca acccaagcac cagcctggcc
     7321 cgcttcgtag ccgtcctctt tccgatcttc ctgccgctcg cgcgctgggc acggcaccgc
     7381 ctcacgttgg cgctcctgac cgccgtcttc ctgcccacca acttcctgat ggccgcgctc
     7441 ttcgtgcgct ggtactgggt cgtctgagcg cgtcgattca ctcgtagcgc gaccgggcgg
     7501 gcacgactcc tgcctggacg attagaatag tgcggtcgag agcagcacgc gcgccggaga
     7561 ccgtgtccgc ttggtggaga ggaagggtat ggccgagttc gacggagcca acgaggggag
     7621 cggcctgcgc ggtaacccgc tgtcgtccct gcgttaccgc gacaaccggg tcttctggat
     7681 tggcacgtcg ttctccagca tcggacaggc cgcctttctg gtcgcgtcga gctggctcgc
     7741 cttccgcctg ggcggcagca gcgcggtcgg catcgtgacc ttcgccacca tgatcccgct
     7801 cttcctggcg acacttgccg gcggggtgct ggcggaccgg caggagcggc gccggctggt
     7861 gctggccagc gaggcggcgc agggcatctt cgccctggtg atcgcctttc aggcgctacg
     7921 cggggccatg agcttcgcgc aattggtcgt gctcgtcttc ctcacgggag tggcccgcgc
     7981 catcgagatg ccaaccgtgc aaaccgtcct gcccagcctg gtgccgcgcg atgagctgct
     8041 caacatcttc tcgctgaaca acctggccaa tcggggctcg cgcttcgtcg gcccggcact
     8101 ggtcgccacc atcctggagc tgcgcggccc ggccccggcc ttcctggtga tcgcgctgct
     8161 ctacctgctg gccatgagcc aggtggtgcg cgtgccgcgc atgcatcagg cggaggtcag
     8221 ccagctcaat ctgcgggagc aggtcgcaga gggcgtgcgc tacatcgccg gccacagccc
     8281 gctggggctc ctgctgctgg tcatcgtgct ccactgtctc ttgacgatgt cgttcgactc
     8341 caccctgccg ctcttcgcca gccagaacct gaagggcaac ggcgccatct actccaccat
     8401 ggtctcggcc atgggattgg gctcgatcat ggcggcgctg ttgctggcca gcatccactc
     8461 gcagcgcgga cgcggcgcgc tcctgctcat cggcgggatc ggcagcggcg cggcaacggc
     8521 gctgatggcg atctcgatga acagcctgct ggcgctgagc gcgatcttcc tggtgggcgt
     8581 gatgaccacc tggttcatga cgctggccaa caccatgatg caggaggcgg tgccggacgg
     8641 gctgcgcggg cgcatctcgg gcatctacct gatgagcgcc agcggggtga tgtccttcgg
     8701 caacctcggc gccggctacc tggccgcgca gtacggctcg gcggcggtgc tcggcctgcc
     8761 ggcgctcggc ttcatcgtgc tgctgctggc cttcagcgtc ctgcgccccg ccctgcgccg
     8821 gctctaccgc gccggctcct tctcgctcga ccaggccggc gccatggagc cggtcagcgc
     8881 gggcgattag aagctcggta caagggaatt gcgaatgtac atacttgcgc ccgccctaca
     8941 gcgcagttcc cctaccttgt ggtggactcg gagtggtgca tgtcatctag tgcaaattgt
     9001 gaacgacgaa ttttgggacc aaggcatgaa gggctcttgc gaatacggcg gtcgcggggc
     9061 aactgccttg cc
//
LOCUS       DASUBI010000180         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_444418, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000180 DASUBI010000000
VERSION     DASUBI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444418"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VFU72_03610"
     CDS             <1..1392
                     /locus_tag="VFU72_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422605.1"
                     /translation="YALGPARQSLTHLRPLPPFYWPPGYPLAVALTSFVAGARPLPGQ
                     LVSLVAGGLAAVFTLLLARELWPTSDMVALPAWGPALAGLVVACGGQLWQSSAVVMAD
                     TLALAAATAGVWALARYGRREQGRWLALAATGLAVAVVTRWAYALVALPCALSALTAL
                     VRRPRPLAVRDSLAALAVVVAILGPIAGPALAGLVQHAALAPFATDLQVYSWQPLNAF
                     RRTFATADGQLAYALPNGLYYASLPARPAYLTPLLAWLILPGLWLLARRRDLALWLLL
                     AGWAASVFAFHAGAPWQNVRFGLAYLPPLAILSTLSFAELWVRANATGRAVLGLLLAA
                     GLAWQVASGTLLLDDFIARKDADLALVRAVNVPPDAQLLTFGPTLTFQHYTALATTDL
                     SEVGPRDLARLLAGQRPTYLLLDVPNVESQWRGLPPDSNYQWLRAGPGLRPLRQYGPY
                     TLFQVGTAPPGTP"
     gene            1389..>2197
                     /locus_tag="VFU72_03615"
     CDS             1389..>2197
                     /locus_tag="VFU72_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422606.1"
                     /translation="MKIGLVVPGFSAHESDWCIPALLDLVRWLTREDDVRVLTLRYPY
                     QPGAYVVYGARVTALGGAARQRLGSALLWRRAFATLSSMHRREPFDVLHAFWATEAGF
                     IAALAGRRLGVPAVISLAGGELARLPDIGYGDQLRRAQRLKIGVALRAATIVTAGSQF
                     LLHRARPWLRSRAADTVHRAPLGVDLDHFQPASAPVQADPPALIHAASLVPVKDQATL
                     LRAATILRERGQRFTLEVAGGGPLEPELRRLTQALDLTNMVRFHGAVPHHDL"
BASE COUNT          258 a          831 c          735 g          373 t
ORIGIN      
        1 tacgcgctcg gccccgcgcg gcagagtttg acgcacttgc gcccgctgcc gccgttctac
       61 tggccgcccg gctacccgct cgccgtcgcg ctcacctcgt tcgtcgccgg cgcacggccg
      121 ctgcctgggc aactcgtcag cctggtcgcg ggcgggctgg cggccgtctt cacactgctg
      181 ctggcgcgcg aactctggcc gacgagcgac atggtggcac tgccggcgtg ggggccggcg
      241 ctcgcggggc tggtcgtcgc ctgcggcggg cagctgtggc aatcgagcgc cgtcgtcatg
      301 gccgacacac tggcgctggc ggcggcaacg gccggtgtct gggcgctggc gcgctacggg
      361 cggcgtgagc agggacgctg gcttgccctg gcggcgacgg gcctcgccgt ggccgtcgtg
      421 acccgctggg cgtatgcgct tgtcgcgctg ccctgcgcgc tctccgccct caccgcactc
      481 gtgcgacgac cgcgcccgtt ggcggtgcgc gacagcttag ccgcactggc cgtcgtggtc
      541 gcaatcctcg ggccgatcgc cggaccggcg ctcgccggcc tggtccagca cgccgcgctc
      601 gcgcccttcg cgaccgacct ccaggtctat tcctggcagc cgctgaacgc tttccgccgg
      661 accttcgcca ccgccgacgg ccaacttgcc tacgcgctgc caaacggcct atactacgcc
      721 agcctgcctg cccgcccggc ctacctgacg ccgctgctcg cctggctcat cctgccgggc
      781 ctctggctgc tcgctcgacg gcgcgacctg gcgctatggc tgctcctcgc tggctgggcc
      841 gccagcgtat tcgcttttca cgctggcgcg ccctggcaga atgtccgctt cgggctggcc
      901 tacctgccac ctctggcaat tctcagcacg ctcagcttcg ctgagctctg ggtgcgcgcg
      961 aacgccaccg gccgggccgt gctgggccta ttgctggccg ccggcctcgc ctggcaggtt
     1021 gcgagcggca cacttctgct cgatgacttc atcgcgcgca aggacgccga tctcgcgctc
     1081 gtgcgcgccg tcaacgtgcc gcctgatgcc cagttactca ccttcggccc aacactgacg
     1141 ttccagcact acactgcgct cgccacgacc gacctctcgg aagttggtcc gcgcgacctc
     1201 gcccgcctgc tcgctggcca gcggcccact tatctcctgc tcgatgtgcc aaatgtggag
     1261 agtcagtggc gcggcctgcc gcccgatagc aactaccagt ggctgcgcgc tgggccaggc
     1321 ttacgtccac tccgccagta tgggccgtat acgctctttc aggtcggcac ggcgccgccg
     1381 gggacgccat gaagattggc cttgttgtcc ctggtttcag cgcgcacgaa agcgactggt
     1441 gtattccggc gctgcttgac ctggtgcgct ggctgacgcg cgaggacgac gtgcgcgtcc
     1501 tcaccctgcg ctatccctac cagcccggcg cgtatgttgt ctatggcgcg cgggtcacgg
     1561 cgctgggcgg cgcggcgcgg cagcggctgg gaagcgccct gctctggcgc cgggcgttcg
     1621 cgacgctatc gtccatgcac cggcgcgagc cgttcgacgt gctgcatgcg ttctgggcga
     1681 ccgaggcggg cttcatcgcg gcgctcgctg gccgccggct gggtgtgccg gcggtcatca
     1741 gcctggcggg tggtgaactg gcccgcctgc cggacattgg ctacggcgac cagctacgcc
     1801 gcgcgcagcg cctgaaaatc ggcgtagcgc tgcgcgcggc gaccatcgtc accgccggct
     1861 cccaatttct gctgcaccgc gcacggccct ggctgcgctc gcgcgcggcg gacaccgttc
     1921 accgcgcgcc gctcggcgtg gacctcgacc acttccagcc ggccagtgcg ccggtgcagg
     1981 ctgacccacc ggcgctcatc cacgccgcct cgctcgtgcc ggtaaaagac caggcgaccc
     2041 tgctgcgcgc ggcgacgatc ctacgcgagc gcgggcaacg attcacgctc gaagtcgcgg
     2101 gcggcgggcc gctggagccg gaattgcggc ggctaaccca ggcgctcgac ctgaccaaca
     2161 tggtgcgctt ccacggcgct gtaccgcacc acgacct
//
LOCUS       DASUBI010000181         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1357614, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000181 DASUBI010000000
VERSION     DASUBI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357614"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFU72_03620"
     CDS             complement(<1..546)
                     /locus_tag="VFU72_03620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5422607.1"
                     /translation="MATVGAVEVAIVGGGIIGCAIAYELARRGVSVALVERGQVGGEA
                     SGTSAGIISPPSSLATPAEKARLTAQSVLAYPAFIEQLGEETGLDTGWRMRGELMVAF
                     GEADRAGLLRVADMQEGLGFQVEWLDGQAARELEPVLPEQVVGGALVREGGSLFSDQL
                     TRAIAEAARRHGARVIESTLVL"
     gene            complement(989..2197)
                     /locus_tag="VFU72_03625"
     CDS             complement(989..2197)
                     /locus_tag="VFU72_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422608.1"
                     /translation="MRDLMRQPVSRRAVLLTPALLALAACGGSGSGRPAAPAAASSPT
                     AHPQVDFTPKPGLARLEAEKPYFGANLDWGNDSAAAFSERLGAPAASYVQFVAFPLGG
                     NEKQILDQFFTQVLAEGGLAVVTLEPHVALGDISPAMAEDLAGFLGGYNARGAGVLVR
                     FAHEMNGTWYAWSQDPAAYVAAFRLVAEAVHRAPGCGMLWAPNYGGGYPFSGGAYQAK
                     PGTPAYDLLDTNHDGTLDEQDDPYAPYYPGDDAVDWVGLSLYHWGNRYPWGANAVPEA
                     NKFADQLSGNYNGQIGDERIVPDFYATYAEGHGKPLAIVETAALFNTTVTGDGELAIK
                     QAWWRQVFDPQVAARFPRIKMINWFEWRKAESEIGGATIDWTATRSQPIREAFRADFP
                     RERFTLGRQA"
     gene            2395..2853
                     /locus_tag="VFU72_03630"
     CDS             2395..2853
                     /locus_tag="VFU72_03630"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEU5422609.1"
                     /translation="MNGVLRGALRGALAGAAATVPMTAVMLGAEAAGLLFVPPPKQIT
                     ANAERQSGVRGQLSQPAFTASWLAAHFGFGTGMGVLYEAARPLLPGPAWLKGLLFGGA
                     VWAVSYTKVLPGLGLYPPPSQDRDTRSATMVAAHAVYGATLAELDQDWRG"
BASE COUNT          406 a         1100 c         1032 g          438 t
ORIGIN      
        1 cagtaccagc gtggactcga tgacccgcgc cccgtggcgc cgcgccgcct cggcgatggc
       61 gcgcgtgagc tggtcgctga agaggctgcc gccctcgcgc accagcgcgc cgcctaccac
      121 ctgctccggg agcaccggct ccagctcgcg cgcggcctgc ccgtcgagcc actcgacctg
      181 gaagccgagc ccctcctgca tgtcggctac ccgcagcagc ccggcgcgat ccgcctcgcc
      241 gaacgccacc atcagctcgc cgcgcatgcg ccagccggtg tcgaggccgg tctcctctcc
      301 cagttgctcg atgaaggcgg ggtaggcgag cacgctctgg gcggtcagcc gggccttctc
      361 cgccggggtg gcgagcgagg acgggggcga gatgatcccc gccgaggtgc cggaggcttc
      421 accccccacc tggccgcgct cgaccagcgc caccgagacg ccccgccgcg ccagctcgta
      481 ggcgatggcg cagccgatga tccccccgcc cacgatcgcc acctcgaccg cgcccactgt
      541 cgccatcacg ccactcctgt tctcgctcat acccgcggca cgttcgcgcg atgggtagca
      601 gatcgcgagc gccctggcaa gcgcggagca ggggatggtg atggatgacg ttggtcgtcg
      661 cccgctaggc cgcatccctt cccctgtcat tctgagcttg tcgaaggatc caacgtggaa
      721 cggccatcaa cgaggtcgat ggccctgttc ccgcgcctac gctcgcgcgc cgggtacggg
      781 cgtggcctgt cgagccggcc gggcgccgca tcagcacgga tccttcgact gcgctcagga
      841 tgaccgggaa cggtcgcagc ctaccggacg gcgaacgcag ccgtcgtctg ccatcctgcc
      901 ccacacgtga ctccccttct cccgcgcatc ggctccgtcc ggagaagggc gggggatgag
      961 ggctccccgg cgggggatga gggcgcgtct acgcctggcg gccgagggtg aagcgctcgc
     1021 gggggaagtc ggcgcggaag gcctcgcgga tcggctggct gcgcgtggcg gtccagtcga
     1081 tcgtcgcccc gccgatctcg ctctccgcct tgcgccattc gaaccagttg atcatcttga
     1141 tgcgcgggaa gcgcgcggcc acctgcggat cgaagacctg ccgccaccag gcctgcttga
     1201 tcgccagctc gccgtcgccc gtcacggtcg tgttgaagag ggcggccgtc tcgacgatcg
     1261 ccagcggctt gccgtgtccc tcggcatagg tggcgtagaa gtcgggcacg atgcgctcgt
     1321 cgccgatctg cccgttgtag ttgccgctga gctgatcggc gaacttgttg gcctcgggca
     1381 cggcgttggc gccccaggga tagcgattgc cccagtggta gagcgagagc cccacccagt
     1441 cgaccgcgtc gtcgcccgga tagtagggcg cgtaggggtc gtcctgctcg tccagcgtgc
     1501 cgtcgtggtt ggtgtcgagg aggtcgtagg ccggcgtgcc gggcttggcc tggtaggcgc
     1561 cgccgctgaa ggggtagccc ccgccgtagt tcggcgccca gagcatgccg cagccggggg
     1621 cgcgatgcac ggcctcggcg accagccgga aggcggcgac gtaggcggcc gggtcctgac
     1681 tccaggcgta ccaggtgccg ttcatctcgt gagcgaagcg caccagcacg cccgcgccgc
     1741 gcgcgttgta gccgcccagg aagccggcca gatcctccgc catcgccggg ctgatatcgc
     1801 ccagcgccac gtgtggctcg agcgtgacca ccgccagccc gccctcggcc agcacctgcg
     1861 tgaagaactg gtcgaggatc tgcttctcat tgccgcccag cgggaaggcg acgaactgca
     1921 cgtagctggc ggccggcgcg cccagccgct cgctgaaggc cgccgcgctg tcgttgcccc
     1981 agtcgaggtt ggccccgaag tagggcttct ccgcctccag ccgcgccagg cccggcttgg
     2041 gcgtgaaatc cacctgcggg tgcgccgtgg gcgaggacgc cgcggcaggc gccgccggcc
     2101 ggccgctgcc gctcccgccg caggccgcca gcgccagcag cgccggggtc aagaggaccg
     2161 cgcgccggct caccggctgc cgcatcagat cacgcatcgg catctgttcc ctcgccgctc
     2221 ccagaccgct cattccgcca ccacgatggc aactcgatgg ttcgccatgc ggccgaggac
     2281 cggcaagaga aggaagtcct gatgacgagg cgagacgcga gcgggcaccc ggtttgcggg
     2341 ggtccgcggc gggtcgccgt gtggcgatcc gccacgaccg agggagcgtg cctgatgaat
     2401 ggagtgctgc gtggcgcgct gcgtggcgcg ctggccggcg ccgcggccac ggtgccgatg
     2461 accgccgtca tgctgggggc cgaggcggcg ggcctcctct tcgtgccgcc gcccaagcag
     2521 atcaccgcca acgccgagcg gcagagcggc gtgcgcgggc agctctccca gccggccttc
     2581 accgccagct ggctggccgc ccacttcggc ttcggcaccg gcatgggcgt gctctacgag
     2641 gccgcgcgcc cgctcctgcc cggcccggcc tggctcaagg ggctcctctt cggcggcgcg
     2701 gtctgggcgg tcagctacac gaaagtgctc cccggcctcg ggctctaccc gccgcccagc
     2761 caggaccgcg atacccgcag cgccaccatg gtcgcggccc acgccgtcta cggcgccacc
     2821 ctggccgagc tggatcagga ttggcgtggc taggtgtgcc cgtcgaacac gttgggcgcc
     2881 atcgaggacg gatccttcga ctccgctctc atctttatac acaacgtgta cgtagcccat
     2941 acgaacgaga cgccgcctgt gccgcttcgc ccgctg
//
LOCUS       DASUBI010000182         4243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_662920, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000182 DASUBI010000000
VERSION     DASUBI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662920"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..2047
                     /locus_tag="VFU72_03635"
     CDS             413..2047
                     /locus_tag="VFU72_03635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5422610.1"
                     /translation="MTAERNPETEEQETTENGFSSFGLNAELMKAIDAAGYEEPTPVQ
                     RETIPLFLEGKDLIVQAQTGTGKTAAFALPILQRLVRDGKQPQAIVLTPTRELAIQVA
                     GTFHQLGKFESAQVVAVYGGQPIERQLRALRHGADVIVGTPGRVMDHLRRETLSLANI
                     TTFVLDEADEMLDMGFIEDIEWILERSPSERQTALFSATMPAPIANLAKKYLRSPVRV
                     SISPERVTVPETEQVYYEVQPRVKVEALTRILDLEQPSSAIIFCRTKREVDELTQRLQ
                     SLGYPSEALHGDLSQMQRDRVMARFRSGQDDILVATDVAARGIDVENVSHVINYDIPG
                     DPESYVHRIGRTGRAGRTGVAVTLVTPRERRMLRLIERATGQRIQQRGVPTIEQIKAK
                     ERELLGESLAEVIESGDLELPMEVVDELAAYYDPSEIAAAAIRMLMREHAPAEYEELT
                     PAQQAETGMTRLYLDLGRKEGIRPMDIVGAIANEAHIPGKSIGQIEITEKASFVDVPE
                     NVAQRVIQALNHTRLRGKPVHAELARPMELRQRHAG"
     gene            2108..3484
                     /locus_tag="VFU72_03640"
     CDS             2108..3484
                     /locus_tag="VFU72_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422611.1"
                     /translation="MRLGIARDVVRRPHRDAVRLAIILLLGLGARLALAPHGGFAGDA
                     QSFRRWAVALVQLPLGDFYASISNADHLPGDLWLLWGVAHLYHLVSPEMDVQGAGFLF
                     LLKLVPALADAGVGAMLFVIARQLAGARAGLLAAAGYVFNPAVIFLSATWGQWDAVSA
                     FVMTVALWLLLRGDLAWALPVLSYATLIKPQIALLFPLLALACWRWSRDGAVVGAPRG
                     FRPWQPVRLALGALASLALFLALSLPFGVGLPLMGTRWNIVERLSFALNRYQSVSLNA
                     FNLWGILGHAGAGQIVNDRRPFLLGVSYHIWGEFLFALAALGALALFAWRPGRLMALW
                     AAFALFFAAFMLPTRVHERYLLPALVLGIALAAVVPAMRWLVAALSLTYLVNIVAVYR
                     LSDAFGFRSASPRVDELVLLVSAVNLFLLLVSYAYGVALARAGEQDEATATRRASGQV
                     VTVAKGQG"
     gene            3491..3964
                     /locus_tag="VFU72_03645"
     CDS             3491..3964
                     /locus_tag="VFU72_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422612.1"
                     /translation="MDVRLPSRWIGASLLLAALVVAAALLTGAARSGTASAADALHAP
                     GFAVAACTRFGGAATADADLRALAWALRLPTDQLVWELSRGRTLAGIIEARGLSAPGV
                     VAAIVRRERAGLVGRVADGQLTQEQEAAMVVSLRARLGQAIAGDVPAGPGCPVRP"
BASE COUNT          598 a         1428 c         1548 g          669 t
ORIGIN      
        1 tcaggcgcgc cgccgcgttc aacaggtcgt cactggtcat ccggcaccct cccgtcactg
       61 aagctcggcc cggtccgcgg ggtccgcgct cccattgtgg tcccggcgaa ggaggaaggt
      121 caaccggcgg gccctccccc caaccccagc ggagcactca cccccggcgg ggagccctca
      181 ccccaacccc tctcccgtgc gcgggcgagg ggctacgaag gaacgcccgt accgagctcc
      241 ccttctccca acgcattggg agaaggggcg ggggggatga gggctccccg gcggggggtt
      301 gagggctccc cggcgggggg ttgagggcgc ctcgccaagt ctggcagccg aattgcacgt
      361 ggagcggcga agtccgctac agttagcggc gacatttagg aggcagagac gcatgaccgc
      421 ggagagaaac ccggaaaccg aggagcaaga gacgacagag aacggcttca gctcgttcgg
      481 gctcaatgct gagttgatga aagcgatcga cgccgccggc tacgaggagc cgacgccggt
      541 gcagcgcgag acgatcccgc tctttctcga aggcaaggat ctgatcgtgc aggcccagac
      601 cggcaccggt aagacggccg ccttcgcgct gccgatcctg caacgcctgg tgcgcgacgg
      661 caagcagccg caggcgatcg tgctgacgcc gacacgcgag ctggccattc aggtcgccgg
      721 caccttccac cagctcggca aattcgagag cgcgcaggtg gtggcggtct acggcggcca
      781 gccgatcgag cggcagctac gggcgctgcg ccacggcgcc gacgtgatcg tcggcacgcc
      841 gggccgggtg atggaccacc tgcgccgcga gacgctcagc ctggccaata tcacgacctt
      901 cgtgctcgac gaggccgacg agatgctcga catgggcttc atcgaagaca tcgagtggat
      961 cctggagcgc tcgccgagcg agcggcagac ggcgctcttc tcagccacca tgccggcgcc
     1021 gatcgccaac ctggctaaga agtacctgcg cagcccggtg cgcgtctcga tctcgccgga
     1081 gcgggtgacg gtgccggaga ccgagcaggt ctactatgag gtgcagccgc gggtcaaagt
     1141 cgaggcgctg acgcgcatcc tcgacctgga gcagccgtcg tcggccatca tcttctgccg
     1201 caccaagcgc gaggtcgacg agctgaccca gcgcttgcag tcgctcggct acccctccga
     1261 ggcgctacac ggcgacctga gccagatgca gcgcgaccgg gtgatggcgc gcttccgctc
     1321 cggtcaggac gacattctgg tcgccaccga tgtggcggcc cgcggcatcg acgtcgagaa
     1381 cgtcagccac gtgatcaact acgacatccc cggcgacccc gagagctacg tgcaccgtat
     1441 cggccgcacc ggccgggccg ggcggaccgg agtggcggtc acactggtca cgccgcgcga
     1501 gcgccgcatg ttgcgcctca tcgagcgggc cactgggcag cggatccagc agcgcggcgt
     1561 gccgaccatc gagcagatca aggccaaaga gcgcgagttg ctgggcgagt cgctggccga
     1621 ggtgatcgag tcgggcgacc tggagctgcc gatggaggtg gtggacgagc tggcggccta
     1681 ctacgacccc tcggagatcg ccgccgccgc cattcgtatg ctaatgcgcg agcacgcgcc
     1741 ggccgagtac gaggagctga cgccagcgca gcaggccgag acggggatga cgcggctgta
     1801 cctcgatctg ggacgcaagg aggggattcg cccgatggac atcgtcggcg ccatcgccaa
     1861 cgaggcgcac atccccggca agagcatcgg ccagatcgag atcaccgaga aggcgtcctt
     1921 cgtggatgtg ccggagaacg tggcgcagcg cgtgatccag gcgctcaacc acacgcgcct
     1981 gcgcggcaag ccggtccacg ccgagctggc ccgccccatg gagctgcgcc agcgccacgc
     2041 ggggtagccc tcacccccgg cccctctccc aatgctgggc gaggggagta tggatgggga
     2101 agggaaggtg cggttgggca tcgcgcgcga cgtcgtgagg cgcccacacc gggatgcggt
     2161 gcggctggcg atcatcctgc tgctggggct gggcgcgcgg ctggcgctgg cgccgcatgg
     2221 aggcttcgcg ggcgatgcgc agtcgttccg gcgctgggcg gtggcgctgg tgcagctgcc
     2281 gctgggcgac ttctacgcct cgatcagcaa cgccgaccat ctgccgggcg atctctggct
     2341 cctctggggc gtggcgcacc tctaccacct cgtctcgccc gagatggacg tgcagggcgc
     2401 cggctttctg ttcttgctca agctggtgcc ggcgctggcc gacgccggcg tcggggcgat
     2461 gctcttcgtc atcgcgcggc agctggctgg cgcgcgggcg gggctgctgg cggccgccgg
     2521 ctacgtcttc aatccggccg tgatcttcct ctcggccacc tggggccagt gggacgcggt
     2581 ctccgccttc gtgatgacgg tcgcgctctg gctgctgctg cgcggcgacc tggcttgggc
     2641 gctgccggtg ctgagctacg cgacgctaat caagccccag atagcgcttc tcttcccgct
     2701 gctggcgctg gcctgctggc gctggtcgcg ggatggcgcc gttgtcggtg cgccgcgcgg
     2761 atttcggccg tggcagcccg tgcggctggc gctgggggcg ctggcctcac tggcgctctt
     2821 cctggcgctg tcgctgccgt tcggcgtggg cctgccgctg atggggacgc ggtggaatat
     2881 cgtcgagcgg ctgtcgtttg ccctgaaccg ctaccagagc gttagcctga acgccttcaa
     2941 cctctggggc atcctggggc acgccggggc cggccagatc gtcaacgacc ggcgtccttt
     3001 cctgctgggc gtcagctacc acatttgggg cgagttcctc ttcgcgctgg ccgcgctcgg
     3061 cgcgctggcg ctgttcgcat ggcgcccagg ccgcttgatg gcgctctggg cggccttcgc
     3121 cctgtttttc gcggccttca tgctgccgac gcgcgtccac gagcgctacc tgctgccggc
     3181 gctggttctg ggcatcgccc tggcggcggt cgtgccggcc atgcgctggc tggtggcggc
     3241 cctctcgctg acctatctgg tcaacatcgt cgcggtctac cgtctctccg acgccttcgg
     3301 ctttcgctcg gccagcccgc gcgtcgacga gctggtgctg ctggtctccg ctgtgaacct
     3361 cttcctgctg ctggtgagct acgcctacgg tgtcgccctg gcgcgcgctg gggaacagga
     3421 tgaggccacc gccacgcgcc gagcgtcggg gcaggtagtc accgtggcta agggacaggg
     3481 gtgaggcgcg atggacgtgc gcctgccgtc gcgctggatc ggggcgagct tgctgctggc
     3541 agcgctggtc gtggcggcag cgctgcttac cggtgcggcc cgcagcggca cggcaagcgc
     3601 cgctgacgcc ttgcatgcgc ccggtttcgc cgtggccgcc tgcacgcgct tcggcggcgc
     3661 ggccaccgcg gacgccgacc tgcgtgcgct cgcctgggcg ctgcgcctgc cgaccgatca
     3721 gctcgtctgg gagctgagcc gcggccggac gctggccggg atcatcgagg cgcgtggcct
     3781 gagtgctccg ggcgtggtcg cggcgatcgt gcggcgggaa cgcgccgggc tggtcgggcg
     3841 cgttgccgac ggccagctga cgcaggagca ggaggcggcc atggtggtga gcctgcgcgc
     3901 ccggctgggg caggcgatcg cgggcgacgt gcccgccggg ccgggctgcc ccgtgcgtcc
     3961 ctagagtggt cggcacaccg gccgtctttt gtagaggcag acctctgtgt ctgccctgtt
     4021 gcccctacgc attggtaacg gagaataggc atcgtcggcg cgaatgacgt ggatcggtga
     4081 taacccaacg agggcagaca cggaggtctg cctctacagg gagaacggca ccgggcccta
     4141 cgcctcgccg cgtgatgtcg gcggcaggac cagcgtgcgc agcaggtggt cgagcgtggc
     4201 ctcggggtcg gcggtcaggc cggagtggac ggccgaggtc tgg
//
LOCUS       DASUBI010000183         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1262667, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000183 DASUBI010000000
VERSION     DASUBI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262667"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VFU72_03650"
     CDS             complement(<1..1588)
                     /locus_tag="VFU72_03650"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5422613.1"
                     /translation="MLTDTEIAASVQPKPITEIAEELGLGPDEIEQYGRYKAKISLDV
                     RKRLATRPNGKYVVVTAITPTPLGEGKTTVTVGLGEGLAYIGKKSIVAVRQASLGPVF
                     GIKGGAAGGGYAQVIPMTDLNLHLTGDAHAVTAAHNLLSAFIDNSLYQGNPLKIDPLT
                     ITWRRVLDVNDRALRQIVIGLGEGNGVPRETGFDITAASEVMAILALSTSYSDLRQRL
                     GRIVVALNTDREPVTAEDIGAAGAMAALMTDALKPNLLQTLEHTPAIVHAGPFGNIAH
                     GNSSVIADLIGLKLGDILVTEAGFGADLGAEKFFDIKCRVSGLVPDAAVVVATVRGLK
                     AHSGRYKVVAGRPLPKEMLEEDLDALQAGLPNLEKQIENVRLFGVPAVVAVNQFPTDT
                     PAEVEMVRQCALRAGALEAVSANVFSEGGKGAAELAEAVMRATERPNAFKFLYQLNEP
                     IQSKIETIATKLYGADTVEYLPTAERSIRQFTRLGYGDLPVCMAKTHLSLSANPKLLG
                     RPSDFSVLVREIRLSAGAGFV"
     gene            complement(1592..2482)
                     /locus_tag="VFU72_03655"
     CDS             complement(1592..2482)
                     /locus_tag="VFU72_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU5422614.1"
                     /translation="MSAMLLEGKPVARDMLARARDEIARLITRTGRAPELAVVRCGAE
                     PDALAYLKAIRRSCRSVALPLREIELPVTIGPDEFGTTIERLNRDPTVAGILVLQPLP
                     RQLPPTLAAQSVSPLKDVDGITIANAGRLFAGLETLVPSTPAGGLAILRHYDIPLAGQ
                     RAVVLGRSLVVGRPMALLLLSADATVTIAHSRSRELASLTREADILVAAVGRPGTVRP
                     EMVKPGATVIDFGVSVRDGVIVGDVDPAVAGVAGALTPVPGGTGVVTNAILIDNVLKA
                     ARWQQRWPAAEAARTAAGEE"
     gene            complement(2482..3126)
                     /locus_tag="VFU72_03660"
     CDS             complement(2482..3126)
                     /locus_tag="VFU72_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEU5422615.1"
                     /translation="MPEETPVDEQSFADQTMRWIAANVADPEHHSGGAVAAALALAAA
                     AASAELVLRLAMRRKANAGRLPVLQADTARLAQLRDDFMAGADHDLATFEQVFRAQRA
                     RKDGEPGAAERLRQALLTAAGSPLALAEQGLELIQIVARQLPVGTRFTISDLGAAAAL
                     ARGAIDASYLTVLANLATTGAAGAEQRRRAESASAEAARLDAEIRQATSERIAG"
     gene            3354..3782
                     /locus_tag="VFU72_03665"
     CDS             3354..3782
                     /locus_tag="VFU72_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5422616.1"
                     /translation="MVQVGQGFAELLKRYREQVRMSQSRLAECSGFDHSYVSRLESGH
                     RTPTRDAVVKFAQALGLPEPERDALLAAAGFMPQRVESLLAEEPILTEVFQLLQSQQI
                     PECVRRDVRDMLQLMVRQARLAALASPSDAVPSPDCLAAD"
     gene            4185..>5087
                     /locus_tag="VFU72_03670"
     CDS             4185..>5087
                     /locus_tag="VFU72_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5422617.1"
                     /translation="MPEFAHLHLHTEYSLLDGLGRIDDYMARAREYGMRHLAITDHGV
                     MYGVVDWYKAARAQELHPVLGIEAYLAPAAVTDRDKSSYHLLLLAENERGYRNLLKLA
                     SRASLEGFYYKPRVDLDMLNELREGVICTSACLGGPVANNFLNEKDEEAERLAGTLTE
                     LFGRDHFYIEIQDHGIEGQRRTNPQLVALARKLDLPLVATNDVHYVKREDAGTQELLV
                     CVQTNTTLDDPKRLRMESDQLYFKSPEEMWRIFGELPQALENTVRIAERCNVELEFGR
                     LHLPDPGIPEGVTPHEHLIQLCRAG"
BASE COUNT          769 a         1712 c         1794 g          812 t
ORIGIN      
        1 aaacgaaccc ggcgccggcc gagaggcgga tttctcggac cagaaccgag aagtcgctgg
       61 gccgcccgag gagctttggg ttggccgaga gcgacaggtg cgtcttggcc atgcagaccg
      121 gcaggtcgcc gtaacctagg cgggtgaact ggcggatgga gcgctcggcc gtcggcaggt
      181 actcgactgt atcggcgccg tagagcttgg tggcgatcgt ctcgatcttg gactgaatcg
      241 gctcattgag ctggtagagg aacttgaagg cattggggcg ctcggtagcg cgcatcaccg
      301 cctcagccag ctcggccgcg cccttgccac cctcgctgaa gacgttcgcc gataccgcct
      361 ccagcgcccc ggcgcgcagc gcgcactggc gcaccatctc gacctcggcc ggcgtatcgg
      421 tagggaactg gttgaccgcg accaccgccg gcacgccgaa gagacggacg ttctcgatct
      481 gcttctccag gttcggcagg cccgcctgta gggcgtcgag atcttcctcc agcatctctt
      541 tgggtaaagg acgtcccgcc acgaccttgt agcggccgct gtgcgccttg aggccgcgta
      601 cggtggccac caccacggca gcatccggca ccagcccgct gacgcggcac ttgatgtcga
      661 agaacttctc ggcgcccagg tcggcgccga aaccggcctc ggtgaccagg atgtcgccga
      721 gtttgaggcc gatcaggtcg gcgatgaccg agctgtttcc gtgggcgatg ttgccgaacg
      781 gaccggcgtg gacgatggcc ggcgtgtgct ccagggtctg cagcaggttc ggcttgagcg
      841 cgtcggtcat cagcgccgcc atggcgccgg ccgcaccgat atcctcggcg gttaccggct
      901 cgcggtcggt gttgagcgcc accacgatgc ggccgagccg ctggcgaagg tcactgtagg
      961 aggtggacag cgccaggatc gccatgacct cggatgcggc ggtgatgtcg aagccggtct
     1021 cgcgcggcac accgttccct tcacccagac cgatcacgat ctggcgtagg gcgcggtcgt
     1081 tgacgtcgag cacgcggcgc caggtgatgg tcagcgggtc gatcttgagc gggttgccct
     1141 ggtagagcga attgtcgatg aaggccgaca ggagattgtg ggcggcggtg acggcgtggg
     1201 cgtcgccggt caggtggagg ttgagatcgg tcattgggat gacctgggcg tagccgccgc
     1261 cggccgcgcc acccttgata ccgaagaccg ggccgagcga tgcctgccgg acggcgacga
     1321 tcgatttctt gccgatgtag gcgagcccct cgccgagccc gacggtgacg gtcgtcttgc
     1381 cctcgcccag cggcgtcggg gtgatggcgg tgacaacgac gtatttgccg ttcggccgag
     1441 tcgcgaggcg cttgcgcaca tccaggctga tcttggcctt gtagcggcca tactgctcga
     1501 tctcgtccgg gcccagcccc agctcctccg cgatctcggt gatcggcttc ggctggacgc
     1561 tcgccgcaat ctcagtatcg gttagcacgc attactcctc acctgcggcg gttcgcgccg
     1621 cttccgccgc cggccaccgt tgctgccagc gcgcagcctt caacacatta tcgatgagta
     1681 tcgcattggt caccacgccg gtcccaccgg gcaccggcgt cagggcgccg gccacgccgg
     1741 ccacggccgg gtcgacgtcg ccgacgatga ctccgtcccg cacgctgaca ccgaagtcga
     1801 tcacggtggc gccgggcttg accatctccg gccggaccgt gcccggacgt ccgacggccg
     1861 cgaccaggat atcggcctcc cgggtcaggc tggccagctc gcggctgcgg ctgtgggcga
     1921 tggtgacggt cgcgtcggcg ctgagcagga gcagcgccat cgggcggccc acgaccaggc
     1981 tgcgccccag cacaaccgcg cgctgcccgg ccagcgggat gtcgtagtgc cgcaggatcg
     2041 ccagcccgcc ggcgggggtg ctcggcacca gcgtctccag cccggcgaag aggcgccccg
     2101 cgttggcgat cgtgatgccg tcgacgtcct tgagcggcga cacgctctgg gccgccagcg
     2161 tcggggggag ctggcgaggc agcggctgca agaccagaat gccggcgacc gtggggtcgc
     2221 ggttcaggcg ctcgatcgtg gtgccaaact cgtcggggcc gatcgtgacc ggcagctcga
     2281 tctcgcgcag cggcagcgcg accgagcgac aggagcggcg aatggccttg aggtaggcca
     2341 gggcgtccgg ttccgcgccg cagcgcacga ccgccaactc cggagcgcga ccagtccgcg
     2401 tgatgagtct cgcgatttcg tcacgcgccc gcgccagcat gtcacgcgcg acgggctttc
     2461 cctccagcag catcgcgctc atcaaccggc gatccgctcg ctggtggcct gccggatctc
     2521 ggcgtcgagc cgggcggcct cggcgctggc cgactcggcg cgccggcgtt gctcggcgcc
     2581 ggctgcgccg gtcgtcgcca ggttggccag caccgtcaga taggaggcgt cgatggcgcc
     2641 gcgtgccagg gcggccgcgg cgcccaggtc gctgatggtg aaacgcgtgc cgacggggag
     2701 ctggcgcgcc acgatctgga tcagctcgag cccttgctcg gccagtgcca gcggcgagcc
     2761 ggcggcggtc aggagcgcct gccgcaggcg ctcggcggcg cctggttcgc cgtccttgcg
     2821 cgcgcgctgc gcgcggaaca cctgttcaaa ggtcgccagg tcgtgatcgg cgccggccat
     2881 gaagtcgtcg cgcagttggg ccaggcgcgc ggtgtccgcc tgcagtaccg gcagccgtcc
     2941 ggcgttggcc ttgcggcgca tggccaggcg cagcaccagc tcggccgagg cggcggcagc
     3001 ggccagcgcc agcgcggccg ccacggcgcc gccggagtga tgctccgggt cggccacgtt
     3061 ggcggcgatc cagcgcatgg tctggtcggc gaacgactgc tcgtcgaccg gggtctcttc
     3121 gggcacgtcc cacacctcct tccatatcag cggagattgt cgcgcctgac gatgtatccc
     3181 ggcaagtcgc ccgcccgtct gtccggttgc tggcgcgatc gtgacaggga gttggcggta
     3241 tagcccgctg gtgtggataa gtggacaacc gcttgacagg gtggcacccg tgggtgtaca
     3301 ttgaagtcaa cggtcgtacg tacattcgct gttagcgaat acaggaggcg ggcatggtcc
     3361 aggtcgggca ggggttcgcg gaactcctca agcggtatcg cgagcaagtg cggatgtccc
     3421 agagccggct tgccgagtgt tccggattcg accacagcta cgtgagccgg cttgagagcg
     3481 ggcaccgtac gcccacccgc gacgcggtgg tcaagttcgc gcaggccctg gggctccccg
     3541 agccggaacg cgacgcgctg ctggcggccg ccggcttcat gccgcagcga gtcgagtcgc
     3601 tgctggccga ggagccgatt ctgaccgagg tctttcaatt gctgcagagc cagcagattc
     3661 ccgaatgcgt gcggcgcgac gtgcgcgata tgctgcagct catggtgcgc caggcgcggc
     3721 tggcggcgct cgcctctccc tcggatgcgg tgcccagccc ggactgcctg gcggcggact
     3781 aggggacggg ggtggtgtcg cccctgccag gtccgactgg agcgggacgt cagcggcgct
     3841 gatgacctgt gacgatttgc ccgggtcttc tccgccgaac tgcaccgggt tgaggcccgc
     3901 gcgagcaggc tgatcgtttc aacgacctgt agccggtgtc gggccggctt cgttctcgcg
     3961 cgcggcggcc tcagcccgaa cggagacact cggtcccgcc gccgttcggc tcgtcgtcgt
     4021 ccgtcggcga tggctctaca atcgcgatgg gctgccaggc cggtcgtacg cgtggattct
     4081 cggggagatc ggcacagccg cgacgaccgg cgctcgtttt tcatagagag tgcgagccga
     4141 gggccgctcg ggcgcaccag tcaatctggg ggaaggtcgc agtcatgccg gagtttgccc
     4201 acctgcacct gcacaccgag tactcgctgc tcgacggact ggggcgcatc gacgactaca
     4261 tggcccgcgc gcgcgagtac gggatgcggc atctggcgat caccgaccac ggcgtgatgt
     4321 acggcgtcgt cgactggtac aaggcggcgc gcgcccagga gctgcacccg gtgctgggga
     4381 tcgaggccta tctggctccg gcggcggtga ccgaccgcga caagagctcc taccacctgc
     4441 tgctgctggc cgagaacgag cgcggctacc gcaacctgct gaaattggcc agccgcgcca
     4501 gtctggaggg cttctactac aagccgcgcg tcgacctcga catgctcaac gagctgcgcg
     4561 agggcgtgat ctgcacctca gcctgcctgg gcggcccggt cgccaacaac ttcctcaacg
     4621 agaaggacga ggaggccgag cgcctggcgg ggactctgac cgagctcttc ggccgcgacc
     4681 acttctacat cgagattcag gaccacggta tcgagggcca gcgccggacc aacccccagc
     4741 tcgtcgcgct ggcgcgcaag ctcgacctgc cgctggtcgc gaccaacgac gtgcactacg
     4801 tcaagcgcga agacgccggt acccaggagc tgctggtctg cgtccagacc aacaccacgc
     4861 tcgacgaccc caagcggctg cgcatggagt cggatcagct ctacttcaag agccccgagg
     4921 agatgtggcg catcttcggc gagctgcccc aggcgctgga gaacacggtg cgcatcgccg
     4981 agcgctgtaa cgtcgagctg gagttcggcc ggctgcacct gcccgacccc ggcatccccg
     5041 agggagtcac gccccacgaa cacctgatcc agctctgccg cgccggg
//
LOCUS       DASUBI010000184         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_772188, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000184 DASUBI010000000
VERSION     DASUBI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772188"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="VFU72_03675"
     CDS             complement(<1..1986)
                     /locus_tag="VFU72_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5422618.1"
                     /translation="MSTPVRRQYLAIKRQYPDVIVFFRLGDFYETFDDDAQLAAQALD
                     ITLTSRELGRDRRIPMAGIPYHAAEGYIAKLIAAGHKVAVVDQLSEPNGRQLVERRVT
                     RVVTPGTITDPAMLDGERNRFIAAALVEGARAGLAVADLSTGEFATTEIVATDAEAAR
                     GAVQRELLRLAPAELVHLGDDALGDVAPDGALPFRSPLERRCWRLDVARETLARHFEV
                     EVLDAFGCAGKPFAIRAAGALLHYLAETQLGSLRQIVDLVTYSTERYMALDAQTRRNL
                     ELSESSRGDRRHALVSVLDATRTPMGARLLRRWLGQPLLDLAGTLDRALVEEPPALLG
                     KGPVFQPGYAPELDDHRRRAREAREWIANLERTERERTGLRTLKVGYNKVFGYYLEIT
                     SAALAAAERDKLARDGTDGVLPDDYLPKQTLANATRYFTPQLKEYETVVLTAEDTLAE
                     LEADAFRKLVRIVAESGSRLLAAAQTIATADVLAALGEVAVERDYTRPVLDESGRIEI
                     AAGRHPVLETTMPRGDYVPNGAQLDPATDQIIILTGPNMAGKSSWLRQVALITLMAQI
                     GSFVPAESARIGLVDRIFTRIGAQDDIATGQSTFMVEMLETANILHHATARSLVVLDE
                     IGRGTSTYDGLAIARAIVEYIHNAPRLGCKTLFATHYH"
BASE COUNT          289 a          739 c          768 g          343 t
ORIGIN      
        1 gtggtagtgc gtcgcgaaga gcgtcttgca accgagccgt ggcgcgttgt ggatgtactc
       61 gacgatcgcc cgcgcgatcg ccagcccgtc gtaggtgctc gtgccccgcc cgatctcgtc
      121 cagcacgacc aggctgcgcg cggtcgcgtg atggaggatg ttcgccgtct ccagcatctc
      181 gaccatgaag gtgctctgac cggtcgcaat atcatcctgc gcgccgatgc gggtgaagat
      241 ccggtcgacc agcccgatcc gcgcggattc cgccggcacg aacgagccga tctgcgccat
      301 cagcgtgatc agcgccacct gccgcagcca ggagctcttg ccggccatgt tcggtccggt
      361 caggatgatg atctggtcgg ttgccgggtc gagctgggcg ccgttcggca cgtagtcgcc
      421 gcgcggcatc gtcgtctcca gcaccgggtg acgcccggcg gcgatctcga tccggcctga
      481 ttcgtccaat acgggccgcg tgtagtcgcg ctccaccgcc acctcgccca gggcggccag
      541 cacatcggcc gtggcgatcg tctgcgcggc ggccagcagg cgcgaaccgc tctccgcgac
      601 gatccggacg agtttgcgga aggcgtccgc ctccagctcg gcgagcgtgt cctcggccgt
      661 cagcacgacc gtctcgtact ccttgagctg gggtgtgaag tagcgcgtcg cgttggccag
      721 cgtctgcttc ggcagatagt cgtccggcag cacgccgtcg gtgccgtcgc gggccagctt
      781 gtcgcgctcg gcggcggcca gcgcggcgct ggtgatctcc aggtagtagc cgaagacctt
      841 gttgtagccg accttcagcg tccgcaagcc ggtccgctcg cgctcggttc gctcgaggtt
      901 cgcgatccac tcccgcgcct cgcgcgcccg ccggcgatgg tcgtccagct ccggcgcgta
      961 gccgggctgg aagaccggcc ccttgccgag cagcgccggc ggctcctcga ccagcgcccg
     1021 gtcgagcgtc cccgccagat cgagcagtgg ctggcccagc cagcggcgca ggaggcgcgc
     1081 gcccatcggc gtcctggtgg cgtcgagcac gctgaccagc gcgtggcggc ggtcgccgcg
     1141 gctgctctcg ctcagctcca ggttgcgccg cgtctgggcg tccagcgcca tgtagcgctc
     1201 ggtgctgtag gtgaccagat cgacgatctg gcgcaggctg ccgagctggg tctcagcgag
     1261 gtagtgcaag agcgctccgg cggcacggat ggcgaagggc ttgccggcgc agccgaaggc
     1321 gtcgagcacc tcgacctcga agtggcgcgc caacgtctcg cgagccacgt ccagccgcca
     1381 gcagcgccgc tccagcgggc tgcggaaggg gagggcgccg tcgggcgcga catcaccgag
     1441 tgcgtcgtcg ccaagatgca ccagctcggc cggggcaagc cgcagcagct cgcgctgcac
     1501 ggcgccccgc gcggcctcgg catcggtggc cacgatctcg gtggtggcga actcgccggt
     1561 cgagaggtcg gccacggcca gcccggcgcg tgcgccctcg accagcgcgg cggcgatgaa
     1621 acggttgcgc tcgccgtcca gcatggcggg gtcggtgatg gtgccgggcg tcaccacgcg
     1681 cgtcacccgc cgctcgacca gttggcgccc gttcggctcg ctcagttgat cgacgaccgc
     1741 caccttgtgg ccggcggcga tgagtttggc gatgtagccc tcggcggcat ggtaggggat
     1801 gccggccatc gggatgcgcc ggtcgcgccc cagctcgcgt gaggtgaggg tgatgtcgag
     1861 cgcctgagcc gccaactggg cgtcgtcgtc gaaagtctcg tagaagtcgc ccaggcggaa
     1921 gaagacgatg acgtcgggat actggcgttt gatcgcgaga tactgacgcc ggaccggcgt
     1981 actcatggca gcgtcgtccc cttgcctctg cccatgcttg cgcgagccgt acggctagac
     2041 tttaccagcc gcgggatgcg ctcgtcgcgt gggggcgggc gcaggtgtca tgcttcgtag
     2101 gagcttggcg ccgcccccgc gcgggactaa agtcgccgc
//
LOCUS       DASUBI010000185         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1030487, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000185 DASUBI010000000
VERSION     DASUBI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030487"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFU72_03680"
     CDS             <1..658
                     /locus_tag="VFU72_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU5422619.1"
                     /translation="IAIVEAVRAAVGPSVDLMVEGHTRFSVASALRVAERLAPSRPAW
                     FEEPTPHLDIDAVVEVARRSPVPVATGESFSSTQQFAELLARDAVHILQPEPLHLGGI
                     WRTRQVFGMADARYAVVAPHSAEGPIATAVCVQLGAATPNFYVAEVFDDFNVGWERDL
                     VTHPIAVVDGYIPIPERPGLGLELNLEELARHPYQPRNFLPLFAPGWERRTGAEHEKP
                     "
     gene            699..1472
                     /locus_tag="VFU72_03685"
     CDS             699..1472
                     /locus_tag="VFU72_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5422620.1"
                     /translation="MSALELIDQIRNELNPAERIPLYEQIAGQLRRAIEAGRLSPGEP
                     IPPEPELARSLGVSRQTVNQALTSLAQRGLLTRRRGVGTFVAEPFIEQPLDGLYTFIR
                     TLTAQGRQPSSRILGYRLTVEEDASRLLTGRDDGLIQEISRLRLVDGEPFVLETIFIS
                     AACGERIAPGRLASEPLYDLMRELCGIDITGAEETLRPVTLEPREAALLGLTTGEPAF
                     LVERTGFSGEEVIELRRSIVRGDRYRYRVWLSTANLDHP"
     gene            1565..>2401
                     /locus_tag="VFU72_03690"
     CDS             1565..>2401
                     /locus_tag="VFU72_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5422621.1"
                     /translation="MGGIELFVVGLFLAAVAAGLLGSLIGLGGGVLIVPILTIGFGLN
                     IHLAIGASIVSVIATSSGAASAYVRDHLTNIRVGMLLELATSLGALTGALVAVYLDAR
                     ILFFLFGGILIFSIVPTLKKLGEDVPRNVIPDRLATRLRLASVYPDRKTGNEIAYEVT
                     NVPTGFGMMYIAGIVSGLLGIGSGALKVLAMDTAMRLPMKVSSATSNFMIGVTAAASA
                     GIYFWRGDVIPIVAAPVALGILVGATVGAKLLVRFQNQTVRKLFLPVLGVVAVEMLLR
                     GTG"
BASE COUNT          358 a          854 c          788 g          401 t
ORIGIN      
        1 gatcgccatt gtcgaggcgg tgcgcgcggc ggtcggcccg tcggtcgacc tgatggtcga
       61 gggtcacacc cgcttcagcg tcgccagcgc gctgcgcgtc gccgagcgac tagcgccgag
      121 ccggcccgcc tggttcgagg agccgacgcc gcacctcgat atcgacgccg tggtcgaggt
      181 ggcgcgccgc agtccggtcc cggtcgcgac cggcgagagc ttctcctcga cacagcagtt
      241 cgccgagctg ctggcgcgcg atgccgtcca catcctgcag ccggagccgc tgcacctggg
      301 cggcatctgg cgcacgcgcc aggtcttcgg catggccgac gcgcgctacg cggtggtggc
      361 gccgcacagc gccgaagggc cgatcgcgac cgccgtctgc gtccagctcg gcgcggccac
      421 gcccaacttc tacgtcgccg aggtcttcga cgacttcaac gtcggctggg aacgcgacct
      481 ggtgacgcac ccgatcgcgg tggtcgacgg ctacatcccg atccccgagc ggccggggct
      541 gggactggag ctcaacctgg aagagctcgc gcgccacccc tatcagccgc gcaacttcct
      601 gcccctcttc gctcccggct gggagcggcg caccggcgcc gagcacgaga agccgtaatg
      661 gactggtcaa catcttcgcg ttaggacata ctcaagacat gagcgcgctg gagctgatcg
      721 atcagatccg taacgagcta aaccccgccg agcgcatccc gctctacgag cagatcgcgg
      781 ggcaactgcg gcgcgccatc gaggccgggc gactgagccc gggcgagcca atcccgcccg
      841 agccggagct ggcccgcagc ctgggtgtca gccggcaaac ggtcaaccag gcgctcacct
      901 cgctggccca gcgcggcctg ctgacccggc ggcgcggcgt cggcaccttc gtggccgagc
      961 ccttcatcga gcagccgctc gacgggctct acaccttcat ccgcaccctg accgcgcagg
     1021 gccgccagcc ctcctcgcgc atcctcggct accgcctgac ggtcgaggag gatgcctccc
     1081 gcctgctaac cgggcgcgac gacgggctca tccaggagat cagccgccta cgcctggtcg
     1141 acggagagcc gttcgtgctg gagacgatct tcatctcggc cgcctgcggc gagcgcatcg
     1201 cccccggccg gttggccagc gagccgctct acgacctgat gcgcgaactc tgcggcatcg
     1261 acatcaccgg agccgaggag acgctgcggc cggtcacgct ggagccacgc gaggcagctc
     1321 tgctcgggct gaccaccggg gagccggcct tcctggtcga gcgtaccggc ttctccggcg
     1381 aagaggtcat cgagctgcgc cgcagcatcg tgcgcggcga ccgctaccgc taccgcgtct
     1441 ggctctccac cgccaatctc gatcacccgt aggccttgcg ctccatccca tttgtctggt
     1501 ataatcctgc ctgacaagac aaaggcgcga tcgacgcctt tggatggagt ggaacggaag
     1561 gtctatgggc ggaatcgagc tctttgtcgt cggtttgttc ctggccgcgg tcgccgccgg
     1621 tctgctcggc tcgctgatcg ggctgggcgg cggtgtgctg atcgtgccga tcctgaccat
     1681 cggcttcggt ctgaacatcc acctggccat cggcgccagc atcgtctcgg tgatcgcgac
     1741 ctccagcggc gcggcctcgg cctacgtgcg cgaccacctg accaacattc gggtcggcat
     1801 gctgctggag ctggcgacct cgctcggcgc gctgaccggc gccctggtcg ccgtctacct
     1861 cgacgcgcgc atcctcttct tcctattcgg cggcatcctg atcttctcga tcgtgccgac
     1921 gctgaagaag ctgggcgagg acgtgccgcg caacgtcatc cccgatcgac tggcgacccg
     1981 cctgcggctg gccagcgtct accccgaccg caagaccggc aacgagatcg cctacgaggt
     2041 cacgaacgtg ccgaccgggt tcggcatgat gtatatcgcc ggcatcgtct cggggctgct
     2101 cggcatcggc tcgggcgcgc tcaaggtgct ggcgatggac accgccatgc gcctgccgat
     2161 gaaggtttcg tcggcgacca gcaacttcat gatcggtgtc acggctgctg ccagcgcggg
     2221 catctatttc tggcgcggcg acgtcatacc gatcgtcgcg gcaccggtcg cgctcggcat
     2281 tctggtcggt gcgaccgtcg gagcaaagct gctcgttcgg ttccagaatc agaccgtacg
     2341 taagctcttc ctaccggtcc tgggagtagt cgcggtggag atgttgttgc gcggcacggg
     2401 g
//
LOCUS       DASUBI010000186         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_950630, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000186 DASUBI010000000
VERSION     DASUBI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950630"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFU72_03695"
     CDS             <1..958
                     /locus_tag="VFU72_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5422622.1"
                     /translation="LGLLVNVVISFFCAVAIEPAVGWLARRGLRRGAATGLVILVVLL
                     AGAGFVLALGSLLVDQISTIVRNLPQYVNDVASWSNRSFHTHLSTGNLRNRVLHSNWV
                     RAYLSHGASNAWGIGATVFGIVFQLFTVLLFTYYMAADGPRLRRELCSVLPPARQAEV
                     LRAWEIAVDKTGGYLYSRALMALISGVAHYILLQVLGVPYAPALGVWVGVVSQFIPTV
                     GTYLAGALPVLIAFAENPWDALWVLGFVIVYQQFENYLLQPRITARTVDMHPAVAFGS
                     VVAGTALLGAVGALIAIPATATLQGFLTAYVRRYEVTDPRAE"
     gene            1144..2130
                     /gene="recA"
                     /locus_tag="VFU72_03700"
                     /pseudo
     CDS             1144..2130
                     /gene="recA"
                     /locus_tag="VFU72_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
BASE COUNT          338 a          758 c          744 g          350 t
ORIGIN      
        1 cctgggcctg ctcgtcaacg tcgtgatctc gttcttctgc gcggtcgcca tcgagcccgc
       61 ggtgggctgg ctcgcacgcc gcggtctgcg ccgcggcgcc gccaccggcc tggtcatcct
      121 ggtggtcctg ctcgcgggcg cgggtttcgt gctggccctc ggttcgcttc tggtggatca
      181 gatcagcacg atcgtgcgca acctgccgca gtacgtgaac gacgtggcct cgtggagcaa
      241 ccggtccttc cacacccacc tgagcaccgg caacctgaga aacagggtcc tccactcgaa
      301 ctgggtgcgc gcgtacctgt cccacggggc cagtaacgcc tggggcatcg gcgccaccgt
      361 gttcggcatc gtgttccagt tgttcacggt gctgctgttc acctattaca tggccgccga
      421 cggcccccgg ctgcgccgcg agctctgctc ggtgctgccg cccgcccggc aggccgaggt
      481 gctgcgcgcc tgggagatcg ccgtggacaa gaccggcggt tatctctact cgcgtgccct
      541 gatggccctg atctccggtg tcgcgcacta catcctgctc caggtgctcg gagtgccgta
      601 cgcgcccgcc ctgggggtgt gggtgggcgt ggtctcgcag ttcatcccga ccgtcggtac
      661 ctacctggcc ggagccctgc cggtcctcat cgccttcgcc gagaacccct gggacgcgct
      721 gtgggtgctg ggcttcgtga tcgtctacca gcagttcgag aactatctgc tgcagccgcg
      781 gatcacggca cggaccgtgg acatgcatcc ggcggtggcc ttcggctcgg tcgtcgcggg
      841 gacggccctg ctgggcgcgg tcggcgcgct catcgcgatc ccggccacgg cgacgctgca
      901 aggcttcctc accgcgtacg tccgccgtta cgaggtcacc gatccgcgcg ctgaatagcg
      961 gcgcagcggc gatcgcgtgt gccgcttgac agaaaaatcg aacatccatt cagatggatg
     1021 caccgggcgg ggcttccgcc ggagttgtcc acagcccggg gccgacgtcc gggcgcgttg
     1081 tcagtggcag gcgctagcgt cgtggacgtg aagcgatcga accagacatc ccgggtggaa
     1141 cccatggcag gaaacgaccg cgagaaggcg ctcgacgccg cgctcgcaca aattgaacgg
     1201 cagttcggca aaggcgcggt catgcgcctg ggcgatcggc cgaatgagcc catcgaggtc
     1261 atctccaccg ggtcgaccgc cctggacgtg gccctcggcg tcggcggtct gccgcgcggc
     1321 cgagtcgtgg aggtctacgg accggagtcc tccggcaaga ccacgctgac cctgcacgcg
     1381 gtggccaatg cccagcgggc gggcggcacc gtggccttcg tggacgccga gcacgccctc
     1441 gaccccgact acgccagggc cctgggcgtc gacacggaca acctcatcct gtcccagccg
     1501 gacaccggcg agcaggccct ggagatcgtg gacatgctgg tccgctccgg cgccctcgac
     1561 ctcatcgtca tcgactccgt cgccgcgctc gtcccgcgcg cggagatcga gggcgagatg
     1621 ggcgacagcc acgtcggtct gcaggcccgc ctgatgagcc aggccctgcg gaagatcacc
     1681 agcgcgctca accagtccaa gaccaccgcg atcttcatca accagctccg cgagaagatc
     1741 ggcgtgatgt tcggctcgcc cgagaccacc accggcggcc gcgcgctgaa gttctacgcg
     1801 tccgtgcggc tggacgtgcg ccggatcgag acgctcaagg acggccagga ggcggtggcc
     1861 aaccgcaccc gggtcaaggt ggtcaagaac aaggtggccc cgccgttccg gcaggccgag
     1921 ttcgacatcg tctacggcga ggggatcagc cgcgagggcg gcctgatcga catgggcgtg
     1981 gagcacggct tcgtgcgcaa gtcaggggcc tggtacacct acgagagcgg ccagctcggg
     2041 cagggcaagg aaaacgcccg ctccttcctg cgtgacaacc ccgacctcgc cgacgagatc
     2101 gagaagcgca tcaaggagaa gctcgggatc gggccggtgc tcgatgcgga ggcaccggca
     2161 cccatgccgg tcgatctgtg agtcgtcggc
//
LOCUS       DASUBI010000187         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_802389, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000187 DASUBI010000000
VERSION     DASUBI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802389"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(550..1755)
                     /locus_tag="VFU72_03705"
     CDS             complement(550..1755)
                     /locus_tag="VFU72_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5422623.1"
                     /translation="MQRRFSATVLVGVAVLSLGFGTLAGGAAGGLAGYWASGRDPATT
                     PAAVLSTVAPTPDHPTPVPTKAPDPTEQPSASRSPGASDAVSVADVVAQVAPAVVTVL
                     NKQVAPGASPGSKPQLMGTGTGFVIDTEGHVVTNNHVVEGSQELSVIFADGTQASAKL
                     LGTDRFADLAVVQVAGPIKATLPFGDSSALRPGEHVIAIGSPLGDFTNTVTDGIISAL
                     HRELETGDGFNMEDMIQHDAPINPGNSGGPLLTLDGKVVGVNTAKVDQAEPGVAAEGL
                     GFAIPSSTVKEITEQIIATGKVERPYLGIVYDTINPRQASSDNLPVDHGVIVREIQPG
                     SPAASADIHPGDIVTKLDDTAIDASNPLVNLLFRHKVGEAVKLEIYRVSDKQTLTVTV
                     TLAPKPDNS"
     gene            1881..2879
                     /locus_tag="VFU72_03710"
     CDS             1881..2879
                     /locus_tag="VFU72_03710"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5422624.1"
                     /translation="MTSARAVDYRCPAVNAVRPLCRRHHYAALALLLTLLVTGCTGAR
                     SFAPTTLYPPESYPGGELLVTSDWLRAHLDDPDLRVVDLSPMRDYDSGHVPGAFHLWW
                     QDTIEVHNDVYGMMVGKDGLRALVRQAGVTPGATVVLYDNAGDRYAARFFWVLRANGF
                     SNVKLLDGGRQAWQAAGFALSRAGASPPAADLPLSLDYSVLIDAKDILAHLHDGSLAI
                     VDNRTVDERQQTWYGRLRLGRIPGAALIPWTALTQDGRVPYYASADALRARFLAAGIT
                     PDKTVVVYGLDGVEAAQSYVALKLLGYPSVKVYDGSWAEWGSRADLPIEPLPGPGA"
     gene            2911..>3979
                     /locus_tag="VFU72_03715"
     CDS             2911..>3979
                     /locus_tag="VFU72_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5422625.1"
                     /translation="MARVAEEEHQTEETPAEGGGAARRTLVVLALGLILAGVVLLAPL
                     HGASPWAHRVMAIITLALVLWIGEGLNIAVTSLLVVGLLAMFGPGGSSKATQSALYGF
                     QLGAPYFLLGTLVIAAATVKSGLAARLARLLVAGTGGSTRRLYAQLVLFIPPFAIAVP
                     SATTRNAILVPAYEHVMRRYGIQRGDTLARLVGLGLGLLQVIGSTAVLTGGVAPITTA
                     FLLGGMSWGQWFVYMAVPNYAIILGAGTLFYLWQRPAPRVRAAPEFEAAREGPWTPAE
                     IRAALIIGTMTLLWLGDFLTGWDPVIPALIGAVALLAPGIGVLDWHEFELSSPWGIFF
                     VTASSLSIAHALETSGAAGWLA"
BASE COUNT          550 a         1377 c         1385 g          667 t
ORIGIN      
        1 gctccccgag ctgatgccgc cgggcatgcg agccgctctc cgtgcggtgg cggccggttt
       61 cggcgcccag gtcgagccgg cggttgcccc ggccacggac tgatgcgtct gggatgacac
      121 ggggccattt tggcaagcgg cgacactcat cgctttcgta cgcccctgct taaccctgtc
      181 atcctgagcg cagtcgaagg atccaagcta cacgcggcac tcgacccgct gactgggcca
      241 ccagcctgcc cggcgcgcga gcgcggacga cgacgagtgg catttgacgg cgttgacggg
      301 cgctccaggt tggatccctc gactgcgctc gggatgaccg gcggagtacg ggctggcgag
      361 gaggctggga cgcggacgac gacctgtctc cgtgtcatcc ccgccgtagt ggcgggatcc
      421 gtcgtggggc ggcaccgcat gacgctgggc cgtgcttaaa aggcaagagg cccgtctggt
      481 ggaccgggcc tcttgcggga taagggggag agtagggggt gggcagcggc gcgactcgct
      541 gcccaggggt caggagttat caggtttagg cgcgagcgtg accgtgacgg tcaacgtctg
      601 tttgtcgctc acgcggtaga tctccaattt caccgcctca ccgaccttgt gccggaagag
      661 caggttgacc agcggattgc tggcgtcgat ggcggtgtca tccagcttcg tgacgatatc
      721 gccgggatga atgtcggcgc tcgcggccgg cgagccgggt tgaatctcgc ggacgatcac
      781 gccgtgatcg accggcaggt tgtcgctgct cgcctggcgc gggttgatgg tgtcgtagac
      841 gatcccgaga tagggccgct ccaccttgcc ggtggcgatg atttgctccg tgatctcctt
      901 gaccgtgctg ctcgggatgg cgaaaccaag tccctccgcc gcgacgcccg gctcggcctg
      961 atcgaccttg gccgtattga cgccgacgac cttgccatcc agcgtcaaga gcggcccgcc
     1021 cgagttgccg gggttgatcg gtgcgtcgtg ctggatcatg tcctccatgt tgaagccgtc
     1081 gccggtctcc aactcgcggt gcagggcgct gatgatgccg tcggtgacgg tgttggtgaa
     1141 atcgcccagc gggctgccga tcgcgatcac atgctcgccg ggccgcagcg cggacgagtc
     1201 accgaaggga agggtcgcct tgatcggccc cgcgacctgc acgacggcca ggtcggcaaa
     1261 ccgatcggtg ccgagcaatt tggcgctggc ctgtgtgccg tccgcgaaga tcaccgagag
     1321 ttcctggctt ccctcaacca cgtggttgtt cgtcaccacg tgcccttccg tatcgatcac
     1381 gaatccggtg ccggtcccca tcaactgcgg cttgctgccg ggcgacgcgc ccggcgccac
     1441 ctgcttgttc aggaccgtca ccaccgccgg cgctacctgc gccaccacgt cggccacgct
     1501 gaccgcgtca gacgcgccgg gtgaacggga ggccgacggc tgctccgtcg gatctggcgc
     1561 cttggtgggc accggcgtgg ggtgatcggg cgtcggagca acggtgctca gtaccgctgc
     1621 cggcgtcgtc gccggatcgc ggccactcgc ccagtacccg gccagcccgc ccgcggcgcc
     1681 acccgccagg gtgccgaagc cgagggagag gacggcgacg ccgaccagaa ccgttgcgct
     1741 gaagcggcgt tgcacccgct gctccttcgg ccggcccggg agtttcctcc ccgaccactg
     1801 ccatcttccg tccgcacgct gaactcgctc ttaaccaaac ctgaaagcaa cctgaaaaat
     1861 cggttcaccc ggacactcgc ttgacgtccg cgcgggcggt tgactatcgt tgccccgctg
     1921 tgaacgccgt gcgcccgctc tgccgccgcc accattacgc cgccctcgcg ctgctgctca
     1981 cgctcctggt gaccggctgc acgggtgcgc gctccttcgc gccgaccacc ctctatcctc
     2041 cggaaagcta ccccggcggc gaactgctgg tcacctccga ctggctgcgc gcccacctgg
     2101 acgatcccga cctgcgcgtc gtggacctga gcccgatgcg cgactacgac agcggccacg
     2161 tgccgggcgc gttccatctc tggtggcagg atacgatcga ggtacacaac gacgtctatg
     2221 ggatgatggt aggaaaggat ggcctgcggg cgctggtccg gcaggccggc gtcacgccgg
     2281 gcgccaccgt ggtcctctac gacaacgcgg gcgaccgcta cgccgcgcgc ttcttctggg
     2341 tgctgcgcgc caacggcttc agcaacgtga agctactcga cggtgggcgt caggcctggc
     2401 aggcggcggg cttcgcgctc agccgtgccg gcgccagccc cccggccgcg gacctgccac
     2461 tctcgctcga ctacagcgtt ttgatcgacg ccaaagacat cctggctcac ctgcacgacg
     2521 gctcgctggc gatcgtcgac aatcgcacgg tggacgagcg gcagcagacc tggtacggac
     2581 gcctgcggct gggccgcatc cccggcgcgg cgctgatccc ctggaccgcg ctgacgcagg
     2641 atggccgcgt gccctactac gccagcgccg acgccctgcg cgcccgcttc ctggcggcgg
     2701 gcatcacgcc ggacaagacg gtggtggtct acgggctcga tggggtggag gcggcgcaga
     2761 gctacgtcgc gctcaagctt ctggggtacc cctcggtgaa ggtctacgac ggcagttggg
     2821 ccgagtgggg cagccgcgcc gaccttccca tcgagccgct tcctggaccc ggcgcgtgag
     2881 ccgggcgagg gtaccaagcg cagggggaga atggcgcggg tagcggaaga agagcaccag
     2941 accgaggaga cgcccgccga gggcggcggc gcggcgcggc gcacgctggt ggtgctggcg
     3001 ctggggctca tcctggcggg cgtcgtgctg ctggcacccc tgcacggcgc cagcccctgg
     3061 gcgcaccgcg tgatggcgat catcaccctg gcgctggtgc tctggatcgg cgaggggctg
     3121 aacatcgcgg tgacctcgct gctggtggtc gggctgctgg cgatgttcgg acccggcggc
     3181 tcgtccaagg ccactcagag cgcgctctac ggcttccagc tcggggcgcc ctatttcctg
     3241 ctcggcacat tggtgatcgc cgcggccacg gtcaagagcg gcctggcggc gcgcctggcc
     3301 cggctgctgg tggccggcac cggcggcagc acgcgccggc tctacgccca gctggtcctc
     3361 ttcattccgc ctttcgccat agcggttccc tcggcgacca cgcgcaacgc catcctggtc
     3421 ccggcctacg agcatgtgat gcggcgctac ggcatccagc gcggcgacac gctggcgcgg
     3481 ctggttgggc tgggccttgg cctgctgcag gtcatcggct cgaccgccgt gctgaccggc
     3541 ggcgtggcgc cgatcacgac cgccttcctg ctcggcggca tgagctgggg gcagtggttc
     3601 gtctacatgg cggtgccgaa ctacgccatc atcctcggtg ccggcacgct cttctacctc
     3661 tggcagcggc ccgcgccacg ggtgcgcgcc gcgcccgagt ttgaggcggc gcgggagggc
     3721 ccctggactc ccgccgagat ccgcgcggcg ctgatcatcg gcaccatgac gctcctctgg
     3781 ctgggcgact tcctgaccgg ctgggacccg gtgatcccgg cgctgatcgg cgcggtggcg
     3841 ctcttggcgc ccggcatcgg cgtgctcgac tggcacgagt tcgagctgtc gtccccgtgg
     3901 ggtatcttct tcgtcaccgc cagctcgctc tcgatcgcgc acgcgctgga gacgagcggc
     3961 gcggccggct ggctggcga
//
LOCUS       DASUBI010000188         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_785625, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000188 DASUBI010000000
VERSION     DASUBI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785625"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..695
                     /locus_tag="VFU72_03720"
     CDS             108..695
                     /locus_tag="VFU72_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU5422626.1"
                     /translation="MDWEIVSRVLSIVLLDLVLSGDNAVVIGMATRSLPSEQRRRAIV
                     FGGAGAVVLRIIFTAVAALLLGVPLLMAAGGLLLLWIASKLVRGGGEAEHHTGGAEKS
                     GMLDAIRTIILADVVMSLDNMLAVGGAAHGSLLLLLFGLALSIPILLLGSGLVARLMN
                     RLPWLVWLGAAILVWTAIGMILDDPLVERYVLSLL"
     gene            762..1262
                     /locus_tag="VFU72_03725"
     CDS             762..1262
                     /locus_tag="VFU72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422627.1"
                     /translation="MAVTTYQRAIEIMQTTPAVLRALVTGLPAEALERAPAEGQWSVR
                     DVLAHMVQAEAGTIGPRIQSMLDQPGAPLPRPNPVEVPATPRAMFDLWDAARQRNLEL
                     FRSVRPEQLDQTGQHARYGAVSLGEHVVEWAYHDLDHLRQMLAALQAELYPEIPTFHN
                     LYPSPV"
     gene            1433..3064
                     /gene="ggt"
                     /locus_tag="VFU72_03730"
     CDS             1433..3064
                     /gene="ggt"
                     /locus_tag="VFU72_03730"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5422628.1"
                     /translation="MASSVATRRPLVRALNGVVATADGLATAAGLEVLQRGGNAMDAA
                     VAAALVTGVTLPQMCGLGGDAFILYYDAKSGEVTGINGSGIAARGCDADYFRAQGLDK
                     VPYRGPHSVSVPGDVDALFVGLDRFGSLPAEELFAPAVRAAEQGFPITHGLANAIANA
                     AQGLREWPTSAAVFLPNGRPLRPGEILRNPNLARTLRAVADGGSEAFYQGQIAREITS
                     YLKQHGGFLSYEDFAEHQSDVYSPPSVDYRGYTVYQTQPPSQGHIMLEELNILENAEL
                     AALGHNSADAIHLMAETKKLAYADRLAYSGDPKFVRIPMEVLLSKSFARDRFQQIDMA
                     QAHEQVGAGRIPERIGDTTYLCVIDRHGNGVSLIHSVSADFGSRVIAGDTGVVLNNRA
                     GRGFTLEAGHPNVFAPGKRTMHTLNCYLVEQQGALRWVGGTPGGDGQPQWNMQALTNL
                     IDYGQDEQEAVESPRWTSFPGTDPLSIANDLVLRVEERVGLETVEALRQRGHRVMVLG
                     AWDGGGAAQVIGRDPESGVLSGGSDPRVEGAALGY"
BASE COUNT          461 a         1060 c         1128 g          491 t
ORIGIN      
        1 cgatcccctc tgcttcttct tcaccgttgt cttcttttgc acaactgcaa tgtgatacaa
       61 tgccggggtt gtcggccgtt cgctcgccgc gtgtgagagg agtgctcgtg gattgggaga
      121 tcgtcagccg cgtactgagc atcgtgctgc tcgacctggt gttgagcggc gacaacgcgg
      181 tggtcatcgg catggcgacc cggagcctcc cgagcgagca gcggcgacga gcgatcgtgt
      241 ttgggggcgc cggcgcggtg gtgctgcgca tcatcttcac cgcggtcgcg gcgctcctgt
      301 tgggtgtgcc gctcctgatg gcggccggtg ggctgctgct cctctggatc gcgtcgaagc
      361 tggtgcgtgg cgggggggag gcggagcacc acacgggcgg cgccgagaag tcggggatgc
      421 tcgacgcgat ccgcaccatc atcctggccg acgtggtgat gagcctggac aacatgctgg
      481 ccgtcggcgg cgcggcgcac ggcagtttgc tgctgctgct ttttggtctg gccctgagca
      541 ttcccatcct gctgctcggc tcgggtctgg tggcgcggct gatgaaccgc ctcccctggc
      601 tggtctggct gggcgccgcg atcctggtct ggaccgccat cggcatgatc ctggacgacc
      661 cgctggtgga gcgctatgtc ctgagcctgc tgtagccgag agcgaagagt cgcgctagag
      721 tcagtgcgac gaatcggtgt gccgatggag ggagccaagt cgtggcggtc acaacctatc
      781 agcgggcgat cgagatcatg cagacgacac cggcggtgct gcgcgccttg gtgaccggcc
      841 tgccggccga ggcgctggag cgcgcgccgg ccgagggcca gtggtcggtg cgcgacgtgc
      901 tggcgcacat ggtgcaggcc gaggcgggca cgatcgggcc gcgcatccag tcgatgctcg
      961 atcagccggg cgccccgctg ccccggccga atccggtcga ggtgcccgcg acgccgcggg
     1021 ccatgttcga cctctgggac gcggcgcggc agcgcaacct ggagctcttc cgctcggtgc
     1081 gtcccgagca gttggatcag acgggccagc acgcgcgcta tggcgcggtc agcctgggcg
     1141 agcacgtggt cgagtgggcc taccacgacc tggatcacct gcgccagatg ctggcggcgc
     1201 tgcaggcgga gctctacccc gagattccga ccttccacaa cctctacccg agcccggtct
     1261 agcgccggtg agcgcgtgga gcaaagcgcg gcccggaacg cgatcgctgc ctgatccacc
     1321 ggcggagaga tccttcgccg cggctcagca tgacggagat tgcgggggga caagcgcgcg
     1381 ggagtgcggt agagtcacgc cattgacaac ccaagcagga gggagagcga ccgtggcttc
     1441 gagtgtggcg acgcggcgtc cgctggtgcg ggcgctcaac ggggtggtgg cgacggcgga
     1501 cggtctggcg accgccgctg ggctggaggt cctgcagcgc ggcggcaacg ccatggacgc
     1561 cgcggtggcg gcggcgctgg tgaccggcgt gacgctgccg cagatgtgcg gtctgggcgg
     1621 cgacgccttc atcctctact acgatgccaa gagcggtgaa gttaccggga tcaacggctc
     1681 gggcatcgcc gcacgcggct gcgacgccga ctacttccgc gcgcagggtc tggacaaggt
     1741 gccctaccgc ggcccgcact cggtctcagt gcccggcgac gtcgacgcgc tcttcgtcgg
     1801 gctcgatcgc ttcggctcgc tgccggccga ggagctcttc gccccggccg tgcgcgcggc
     1861 cgagcagggc ttcccgatca cccatgggtt ggccaacgcg atcgccaacg ccgcccaggg
     1921 gctgcgcgag tggcccacct cggcggcggt attcctgccc aacggccgtc cgctgcgacc
     1981 cggcgagatt ctgcgcaacc cgaacctggc gcgcaccctg cgggcggtcg ccgacggtgg
     2041 ctccgaggcc ttctaccagg gccagatcgc gcgcgagatc acctcgtacc tcaaacagca
     2101 cggaggcttc ctctcgtacg aggacttcgc cgaacaccag tccgacgtct acagcccgcc
     2161 ctcggtcgac taccgcggct acaccgtcta ccagacgcag ccgcccagcc aggggcatat
     2221 catgctggag gagctgaaca tcctggagaa cgccgaactg gccgcgctgg ggcacaactc
     2281 ggccgacgcc atccacctga tggccgagac caagaagctg gcctacgccg atcggctggc
     2341 ctactccggc gatcccaagt tcgtgcgcat acccatggag gtgctcctct cgaagtcgtt
     2401 cgcccgcgac cgcttccagc agatcgacat ggcccaggcg cacgagcagg tcggcgccgg
     2461 gcgcatcccc gagcgcatcg gcgacaccac ctacctctgc gtcatcgacc gccacggcaa
     2521 cggcgtctcg ctgatccaca gcgtctcggc ggacttcggc agccgcgtga tcgccggcga
     2581 caccggcgtg gtgctgaaca accgcgccgg gcgtggcttc accctggagg cgggccaccc
     2641 caacgtcttc gcgccgggca agcgcaccat gcacacgctg aactgctatc tggtggagca
     2701 gcagggcgcc ttgcgttggg tcggcggcac gccgggcggc gatggccagc cgcagtggaa
     2761 catgcaggcg ctgacgaact tgatcgacta cggccaggac gagcaggagg cggtcgagtc
     2821 cccgcgctgg accagcttcc ccggcaccga cccgctctcg atcgccaacg acctggtgct
     2881 gcgggtggag gagcgcgtcg gcctggagac cgtggaggcg ctgcgccagc gcgggcatcg
     2941 cgtgatggtg ctgggcgcct gggacggcgg cggcgcggcg caggtgatcg gtcgcgatcc
     3001 ggagagcggc gtcctctccg gcggctccga tccgcgcgtc gagggcgcgg cgctggggta
     3061 ttagcgggaa gccctgttcc cagccgggtc gccctcatcc ccagcccttc tccggacgga
     3121 gccgatgcgc gggagaaggg
//
LOCUS       DASUBI010000189         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_812497, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000189 DASUBI010000000
VERSION     DASUBI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812497"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFU72_03735"
     CDS             <1..1666
                     /locus_tag="VFU72_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422629.1"
                     /translation="DLRSARMIAKYEQLRASEGIDPQVSAMLADEKQRQLDLERRLQE
                     ALADTLASGEVIFRGVTWDGSALGDNLSRAVNALLDRTVPELYPKLEMGARTVTGKEA
                     EEILKAANLNALPQVYYDPPMGLGLVRSDAAGRKAIDEEAEIAREVLAYLRQQHSYGE
                     KVTGRLLENHFTGLGYGWNPDVLHIVLAALLRAGAIEVSYQGRRYRNHIDPLVRQPFS
                     GTQAFRSAGFAPREKVDLKSLVRAAENLESLTGEDVDIEEEAIAGRLKALARQELEGI
                     GPALARINANKLPRSEVLTEYRDALRAIVEAAPDDCVRTLAGEGKTLRDERDQVRAIE
                     DAVSDAELPKLLGASRALHELLPELRRRGLSAEVAEAGGRLEALFNGGEVYSRPGELV
                     GLALRIRGAYEQLYANLHAERLQVYEAAIQELAERPEWRELEGLDDDSRLATLREAIL
                     ADLKRRACDSRAINPETERCRACHAGLGQLESDIEAIGGLRQRALEQLWKAVEPEAPD
                     TPSKRLRAVDYVHGPIASEEDLNAFLERLKAEIIAALRAGGRVILE"
     gene            1790..>2409
                     /locus_tag="VFU72_03740"
     CDS             1790..>2409
                     /locus_tag="VFU72_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422630.1"
                     /translation="MERELRNALRLAVTRCRTLLEEDFSGQLEGEYGVYAGGRVADED
                     ALPQLDAEGLRARRAIVAAIAHRRAGGVKPAEAVTQFVRESAFSTLNRLTALKLMEAP
                     ERRLVQQSLGDGASSSGFRQFLLLSPEAMRPEPDGGYRLYLELLFQDLSAEIGVLFDL
                     DLPQGILFPRERTLRALLEELNAPALAKVWGEDETLGWVYQYFTPK"
BASE COUNT          387 a          826 c          875 g          321 t
ORIGIN      
        1 cgacctccgg tctgccagga tgatcgcgaa atacgagcag ctccgcgcca gcgagggcat
       61 cgacccgcag gtctcggcca tgctggccga cgagaagcag cggcagctcg atctcgagcg
      121 ccggctgcag gaggcgctgg ccgacacgct ggccagcggc gaggtcatct tccgcggtgt
      181 cacctgggac ggctccgccc tcggcgacaa cctcagccgc gcggtgaacg cgctcctgga
      241 tcggaccgtg ccggagctct accccaagct cgagatgggc gcgcgcaccg tcaccggcaa
      301 ggaggcggag gagatcctga aagcggccaa tctcaacgcg ttgccgcagg tctactacga
      361 cccgcccatg gggctcggcc tggtgagaag cgacgccgcc ggccgcaagg ccatcgacga
      421 ggaggcagag atcgcccgcg aggtgctcgc ctacctccgc cagcagcaca gctacggtga
      481 gaaggtcacc ggccggctgc tggagaacca cttcaccggc ctcggctacg gctggaatcc
      541 cgacgtgctg cacatcgtgc tagccgccct gctgcgggcc ggcgccatcg aggtcagcta
      601 ccaggggcgc cgctatcgca accacatcga cccgctggtg cggcagccct tcagcggcac
      661 ccaggctttc cgctcggccg gcttcgcccc acgcgagaag gtcgacctca agagcctggt
      721 cagagcggcc gagaacctgg aatcgctgac cggcgaggat gtcgacatcg aagaggaggc
      781 gatcgccggc cggctgaagg ctttggcgcg gcaggagcta gaggggatcg ggccggccct
      841 ggccaggatc aacgccaaca agctgccgcg cagcgaggta ttgaccgagt atcgcgatgc
      901 cctgcgcgcg atcgtcgagg cggcgccgga cgactgcgtg cgcacgctgg ccggcgaggg
      961 gaagacgctg cgcgacgagc gcgaccaggt acgggcgatc gaggacgccg tttccgacgc
     1021 ggagctgccc aagctcctcg gcgccagccg ggccctccac gagctgctgc cggagctgcg
     1081 ccgccgtggc ctgagcgccg aggtggctga ggcgggcggc cgtctggaag ccctcttcaa
     1141 cggtggcgag gtctacagcc gccccggcga gctggtcggt ctggcgctgc gcatccgcgg
     1201 ggcctacgag cagctctacg ccaacctcca cgccgagcga ctgcaggtct acgaggcggc
     1261 catccaggag ttggccgagc gcccggaatg gcgcgagctg gaggggctgg atgacgacag
     1321 ccgcctcgcc acgctgcgtg aggccattct ggccgatctc aagcgccgcg cgtgcgacag
     1381 ccgggcgatc aatcccgaga ccgagcgctg ccgggcctgc catgccggcc tcggccagct
     1441 tgagtccgac atcgaggcga tcggcggctt gcgacagcgc gcgctggagc agctctggaa
     1501 agcggtcgag ccggaagcgc ctgatacgcc gtccaagcgg ctgcgcgcgg tcgactacgt
     1561 ccacggcccg atcgccagcg aggaggacct gaacgccttc ctcgaacggc tcaaggccga
     1621 gatcatcgcg gctctgcgcg ccggcggccg cgtgattctg gaataggagg ggcgtgacga
     1681 tgatgccggt actacccgtc gcgcgcgtgg cgctggcctg tgaagcgctg gtcgagcagc
     1741 agatgcgcga gtaccgcaca ctgctggcgg cggcctgagc ggccggcgga tggagcgaga
     1801 gctgcgcaac gccctgcggc tggcggtgac gcgctgccgg acgctgctgg aggaggactt
     1861 cagcggccag ctggagggcg agtacggcgt ctacgccggc ggacgcgtcg cggacgagga
     1921 cgccctgccg cagctcgacg ccgaggggct gcgggcgcgg cgggcgatcg tggcggccat
     1981 cgcgcaccgg cgggccggcg gggtaaagcc cgccgaagcc gtgacgcagt tcgtgcgcga
     2041 gtcggccttc tcgacgctga accggctgac cgcgctcaag ctgatggagg cgcccgagcg
     2101 gcggctggtc caacagtcgc tcggcgacgg agcgagctcc agcggcttcc ggcagttcct
     2161 gctcctctcg ccggaggcga tgcgtcccga gccggacggc ggctaccggc tctacctgga
     2221 gcttctcttc caggatctct ccgccgagat cggcgtcctc ttcgacctcg atctgcccca
     2281 gggcatcctc ttcccgcgcg agcgcacgct gcgggcgctg ctggaggagc tgaacgcgcc
     2341 ggcgctggcg aaggtctggg gcgaagacga aacgctcggc tgggtctacc agtacttcac
     2401 cccgaagga
//
LOCUS       DASUBI010000190         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_294540, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000190 DASUBI010000000
VERSION     DASUBI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294540"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFU72_03745"
     CDS             <1..410
                     /locus_tag="VFU72_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422631.1"
                     /translation="RMGLKWANPLSVGSFSIVSAAHALARTTGEFVLARVGLGVTEAL
                     ATPAVIKTVGECFEPEPRARALGWINASNSVGAIITPLVVPALALAVGWRAAFAAVGA
                     VGLLWTAAWLWLMRSFDSGARPPALPAMAAVRR"
     gene            534..1013
                     /locus_tag="VFU72_03750"
     CDS             534..1013
                     /locus_tag="VFU72_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422632.1"
                     /translation="MRALGSPVAAVYLAAIIGSVGGGSASSRLIAGGLRVVRARFAVM
                     LACSALAFALFAVMDVHWLWGAVALLGLGIVAHQGFSVNLFALITDVFEPERVGTITS
                     LGALSGNLAGMAVVWLLGVHLTAGGRYTPFLDFAAISYALALLWLWLLLPRLLRNAA"
     gene            1010..2020
                     /locus_tag="VFU72_03755"
     CDS             1010..2020
                     /locus_tag="VFU72_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5422633.1"
                     /translation="MSETRVLRWGILGAARIALRHVLPAFGSAPHAHAAAIAARDPRR
                     ARAAAEKFGIGRTYETYEALLADPDIDAVYVPLPNDLHMPWTVRALEAGKHVLCEKPI
                     ALDAGEARAIAAAAAAQGSQVAEAYMIRFHPQWQRAKALAAAGALGELRAIQCWFAYD
                     NPADELRNSPRHGGGGLYDIGGYALVAARWLFGTDPLRLIGSFHRESAGGVDCLMSGL
                     AEFPGGRQLGFGVSTRLPRLQTVIAHGTRARLTIETPFNAAADRPTRLLIDDGRDLYG
                     GGVRVETIPPADQYALQLEAFSLAVLEGRPPLFGIDDALVNMRAVDALFRSERSGAWE
                     RP"
     gene            2184..>2701
                     /locus_tag="VFU72_03760"
     CDS             2184..>2701
                     /locus_tag="VFU72_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU5422634.1"
                     /translation="MSVAAANLGLPPPPDATVLFDGRGLGQWNLAADPGGGWRQSDGL
                     MLPGGKVFNWLFTKRSFGSVQLHLEFREPHPARGAGQERGNSGVRLMGAYEIQILDAD
                     HDSTYADGVLGAVYGQTPPSVLPVMASGTWQSLDILFDAPRFQGNRLVRPPYVTVMVD
                     GIVVQDHQEIFG"
BASE COUNT          337 a          935 c          961 g          468 t
ORIGIN      
        1 accgtatggg gctcaaatgg gccaacccgc tcagcgtcgg ctccttcagc atcgtgtcgg
       61 cggcgcacgc gctcgcacgg accacaggcg agttcgtgct ggcgcgcgtc ggcctcggcg
      121 tgaccgaggc gctcgccact cccgcggtga tcaagaccgt gggcgagtgc ttcgagcccg
      181 agccgcgcgc ccgggcgttg ggttggatca acgcctccaa cagcgtcggt gccatcatca
      241 caccgctcgt cgtaccggcg ttggctctgg cggtcggctg gcgcgcggca ttcgcggccg
      301 tcggtgccgt cggcctgctc tggacggctg catggctgtg gctgatgcgc tcgttcgact
      361 cgggagcgcg gccgccggca ctgcccgcca tggcggctgt gcgccgctag ctgcgcctgc
      421 tcgccgagag gcgcacctgg gcgatcctcg gcgccaaggc gctgatcgac caggtgtggt
      481 ggctgttgct cttctggact ccggactttc tgcaccgtga tttccacctg tccgtgcgcg
      541 cgctgggctc gccggtcgcg gccgtctacc tggccgcgat catcggctcc gtgggtggcg
      601 gctcggcatc ctcccgcttg atcgccgggg ggctgcgcgt cgtgcgcgcc agattcgcgg
      661 tgatgctggc ctgctcggcg ctggctttcg cgctcttcgc cgtgatggat gtgcactggc
      721 tctggggggc ggtcgcgctg ctcggcctcg gcatagtggc ccatcagggc ttctcggtga
      781 acctcttcgc cctgatcacc gacgtgttcg agcccgagcg ggtcggcacg atcacgagcc
      841 tcggcgcgct gagcggcaat ctcgccggga tggcagtcgt gtggctgcta ggcgtgcacc
      901 tgacggccgg cggacgctac acgccctttc tcgacttcgc cgcgatctcg tatgcgctgg
      961 cgctgctgtg gctgtggctg ttgctgccgc ggctgctgcg aaatgcagca tgagcgagac
     1021 ccgggtactc cgttggggca ttctcggggc cgcgcgcatc gcgctgcgcc acgtgctgcc
     1081 ggcgttcggc tcggcgccgc acgcccatgc ggcggccatc gccgcccgtg accctcggcg
     1141 cgcgcgcgca gcggcggaga agttcggcat cgggcgcaca tatgagactt acgaggcgct
     1201 gctcgcggat cccgacatcg acgccgtcta tgtgccgctg ccgaacgatt tgcacatgcc
     1261 ctggaccgtc cgggcgctcg aggccggcaa gcatgtcctc tgcgagaagc cgatcgcgct
     1321 cgacgcgggt gaggcgcgcg cgatcgcggc ggcggccgcc gcccagggat ctcaagtggc
     1381 ggaggcgtac atgatccgct tccatccgca gtggcagcgg gcgaaggcgc tcgcggccgc
     1441 cggagcattg ggagagttgc gggcgatcca gtgttggttc gcgtatgaca atccggccga
     1501 cgagctgcgc aacagcccgc ggcacggcgg gggcggcctc tacgacattg gcggctacgc
     1561 gctggtggcg gcgcgctggc tcttcgggac cgatcccctg cggctgatcg gctcctttca
     1621 ccgggagtcc gccggcggcg tcgattgcct gatgagcgga ctcgcggagt tccctggcgg
     1681 gcggcaactc ggtttcggcg tctcgacccg tctgccgcgg ctgcagacgg tgattgccca
     1741 tggcacgcgc gcccgtctga cgatcgagac acccttcaac gcggccgccg acaggccgac
     1801 gcggctgctg atcgatgatg gccgcgatct ctacggcggc ggcgtgcggg tcgagacgat
     1861 cccgccggcc gatcagtacg ccctgcagct cgaggcgttc tccctcgccg tgctggaggg
     1921 tcggccgcct ctcttcggca tcgacgatgc gctcgtcaac atgcgcgccg tggatgcgct
     1981 gtttcggtcg gagcgctccg gcgcctggga gcggccatga gcggcatcgc cgccgtcagc
     2041 gtcctgtacc gcgaagaaac ccgatgatga tgcgtgcgct cagggctttc atcccctgcg
     2101 ccattctcct ggccgtgctg ccggcgccgg ccgccacaca accctggccg cccaacgatc
     2161 cgagccggcc gcagccgccg cgcatgagcg tcgccgcggc gaatctcggg ctgccgccgc
     2221 cgcccgatgc gacggtgttg ttcgatggcc gcggtctcgg gcagtggaac ctcgcggccg
     2281 acccgggcgg cggatggcgt cagtcggacg gcctgatgct ccccggcggc aaggtcttca
     2341 actggctgtt caccaagcgc agcttcggca gcgtgcagct gcacctggag ttccgcgagc
     2401 cccatccggc gcgcggcgcg ggccaggagc gcggcaacag cggggtgcgc ctcatggggg
     2461 cgtatgagat ccagatactg gatgccgatc acgactcgac ctatgccgac ggcgtattgg
     2521 gcgcggtcta cggccagaca ccgccgagcg tgctgccggt catggcctcc ggcacgtggc
     2581 aaagcctcga tatcctcttc gatgcgccgc ggttccaggg caatcggctc gtccgcccgc
     2641 cctacgtgac ggtcatggtg gacggcatcg tggtgcagga ccaccaggag atcttcggca
     2701 a
//
LOCUS       DASUBI010000191         5725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1003300, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000191 DASUBI010000000
VERSION     DASUBI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003300"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFU72_03765"
     CDS             <1..324
                     /locus_tag="VFU72_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEU5422635.1"
                     /translation="LVISKLPFSVPSDPVFSARSEGFDNPFNDYAVPQAILRFKQGFG
                     RLIRSSADRGVCVILDRRIVSRRYGGAFVSSLPECTVEVGGVQQMPARAERWLDRSRL
                     DTIMR"
     gene            357..1334
                     /gene="glpX"
                     /locus_tag="VFU72_03770"
     CDS             357..1334
                     /gene="glpX"
                     /locus_tag="VFU72_03770"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU5422636.1"
                     /translation="MSYTIDRNLALELVRTTEAAALSASRWMGRGDKIAADQAAVDGM
                     RRMLSTVQINGVVVIGEGEKDEAPMLYIGEEVGAGGPAVDIAVDPIDGTTLLSKGMPN
                     SIAVIAVADRGSMYYPPHIVYLNKISTGAEAAAAIDINASVADNLQAIAEAKRMNVRD
                     LTVVVLERPRHEQLIAEIRAVGSRIKLISDGDVAGSLMAATPGTGIDVLMGIGGSPEG
                     VISAAAMKSLGGAIQAKPWPRNEDERRQAEAAGVELNRVLTTDDLVHSDNVFFAATGI
                     TDGEVLQGVKYFSDTVTTQSLVMRSRSGTVRMVDATHRWTKVRTIFGEA"
     gene            1804..3057
                     /gene="rho"
                     /locus_tag="VFU72_03775"
     CDS             1804..3057
                     /gene="rho"
                     /locus_tag="VFU72_03775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5422637.1"
                     /translation="MTVTISDLEHKTLPELQDIAKELDVSGYSRLRKQELINRVLQVQ
                     TEQSGNIYGEGVLDIMDDGYGFLRGERYLPGPNDVYVSQSQIRRFGLRTGDWISGHVR
                     PPKESEKYFSLLRVEAVNGMDPEAARRRPNFDNLTPIFPLELIDLETEPNILSTRLLN
                     MVAPIGRGQRGLIVSPPKAGKTLLLKAIANGITTNYADVHLIVCLIGERPEEVTDMKR
                     SVAGEVVSSTFDEPVEDHTKVAEMTLERAKRLVECGRDVVILLDSITRLARAYNLAVP
                     PSGRTLSGGIDPVALYPPKRFFGAARNIEGGGSLTIVATCLIDTGSRMDDVIYEEFKG
                     TGNMELHLDRKLAERRIYPSIDIQRSGTRREELLLEDQVLRQVWTMRRMVSMLGGTEG
                     TELVLGRMAKTGSNAEFLATLNKDL"
     gene            complement(3209..4708)
                     /locus_tag="VFU72_03780"
     CDS             complement(3209..4708)
                     /locus_tag="VFU72_03780"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5422638.1"
                     /translation="MIELLASAPLVGGVEPIPVEGQTDADLVLRSLLDPDHDTIVLAS
                     QPLAPVTSPRLWLPGLTRQQLDDLLGGSITDWAKVGVPTATPVQPVAIGGVAVSRAAP
                     ATIQVPDYAGFLALLPQHPGALALAPLDVVDFQAGSPPVDDVRLLAETTPTGRAPLAL
                     ALVATGSGPVWEQLKPWLAAQSGALAGGRPAPTPLTVTFSGDVILGRTVHTIISRLGD
                     FAAPFRKVADELKGADLTILDLECTLSDTITPPTDPYTFSFMTRAAAADGLVLAGVDA
                     VSQANNHSMNFGADGMRDTLAALDARKILHFGIGEQLSQARQPVILQAKGVKLALLGF
                     DGITGDTYGATGDSAGTSPLVADNLVADIQAVRPKADLVIPFIHWGVEYTLTPTDGQV
                     LIAHQAIDAGADMVVGSHPHWVQGVETYHGRPIVYSLGNFVFDQEWSLETKQGLIMHL
                     IFRGRRLAGLRFVPIMIEDYYQPRLAEGDEISTILDRVWRSSDILAGTA"
     gene            5103..>5725
                     /locus_tag="VFU72_03785"
     CDS             5103..>5725
                     /locus_tag="VFU72_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422639.1"
                     /translation="MRSQGGHRRADYSRARRIHVLLGPLLALALLASGLAPRSSAASA
                     PAKVYFPVTGHHVSDQVLTYWRAHGDLSIFGYPLTEPIQRDGLTVQYFERARLELHPE
                     LAGTPYEVELTLLGDQTAAGHQEASFQRMSAVGANDSKDRVYFSATGHYLAYGFKSFW
                     DGNGGLAIFGYPISEEFSEKNADDGKTYTVQYFERARFEYHPEFKGTP"
BASE COUNT          984 a         1809 c         1977 g          955 t
ORIGIN      
        1 ctggtgatca gcaagctccc cttctcggtg cccagcgacc cggtcttctc cgcccgcagc
       61 gaggggttcg acaatccgtt caacgattat gccgtgccac aggcgatcct gcgtttcaag
      121 cagggctttg gccgcctgat ccgcagcagc gccgaccgcg gagtctgcgt catcctggat
      181 agacgcatcg tcagccggcg ctacggcggc gcgttcgtca gctcgctccc cgagtgcact
      241 gtcgaggtcg gcggcgtaca gcaaatgccg gcacgggcgg agcgctggct cgaccgttcg
      301 cggttggaca cgatcatgcg atagcggctc gcggtcggcg agcagggagg cagaccatga
      361 gctacacgat cgatcgcaac ctggcgctgg aactggtgcg caccaccgag gcggcggcac
      421 tctcggcctc gcgctggatg gggcgcggcg acaagatcgc ggccgatcaa gcggcggttg
      481 acggcatgcg ccgcatgctc agcaccgtcc agatcaatgg cgtggtcgta atcggcgagg
      541 gcgagaagga cgaggcgccg atgctctaca tcggcgagga ggttggcgcg ggcggcccgg
      601 cggtcgatat cgcggtcgat ccgattgacg gtaccaccct gctctccaag gggatgccga
      661 actcgatcgc ggtgatcgcg gtggctgacc gcggctcgat gtactacccg ccgcacatcg
      721 tctatctcaa caagatctcg actggagccg aagcggccgc cgccatcgac atcaacgcgt
      781 cggtagccga caacctgcag gcgatcgcgg aggccaagcg gatgaacgtg cgcgacctga
      841 ccgtagtcgt gctggagcgc ccgcgccacg agcagttgat cgcggagatt cgagcggtcg
      901 gctcgcgcat caaactcatc agcgacggcg acgtggccgg ctcgttgatg gcggccacgc
      961 cgggcacggg gatcgacgtg ctgatgggga ttggcggctc accggagggt gtcatttcgg
     1021 cggcggcgat gaaatcgctg gggggcgcca tccaggccaa gccgtggccg cgcaacgaag
     1081 atgaacggcg gcaggccgag gcggccggtg tcgagctgaa tcgggtgctg acgaccgatg
     1141 acctggttca ctccgacaat gtcttcttcg ccgccaccgg catcaccgac ggagaagtgc
     1201 tgcagggcgt gaagtatttc agcgacacgg tgacgaccca gtcgctggta atgcgttcgc
     1261 gctcgggcac ggtgcgcatg gtcgacgcca cgcaccgctg gaccaaagtc cgcaccatct
     1321 tcggtgaggc ttgacgcgat ttcggcgccg tgatatagtg gctgcggtgt cattggatgt
     1381 cgccgcagcc ccttctgccg ctagagcttc cgcgtcacta ctgtctagcg gtgttttcaa
     1441 aggtttcttg atgcgatcga gccccagtgc cctgagccac gagtgtgtcc ctgcgcggcg
     1501 ggcccgggtc gcatcagttc gaggaacgag atcggtcacc agccaaagat gagccgggca
     1561 agagacggaa cgacgcatcg cttgcgtgga cgaccgtccg gcgcggggcc gcccctgtct
     1621 atccccactc gctccccgtg cccacgacga tcaggacggc gttcattcct cgggacggcg
     1681 tgggagcgtg tccagacgcc ctgcacccat tcaacgatca acaaccggac tatgcaataa
     1741 ccgcgttccg ccatcctcgg gcgcgctgcc ccggccggaa acgcgaagca caaaggaacg
     1801 acaatgaccg ttaccatctc ggatctggag cacaagacgc taccggagct ccaggatatc
     1861 gccaaggagc tcgatgtctc cggctactcc cgcctgcgca aacaggagtt gatcaaccgg
     1921 gtactgcagg tgcagaccga acaatccggc aacatctatg gcgagggcgt gctcgacatc
     1981 atggacgacg gctatggctt cttgcgcggc gagcgctatc tgccggggcc gaacgacgtc
     2041 tacgtctcgc agtcgcagat ccggcgcttt ggtttgcgca ccggcgattg gatctccggc
     2101 catgtgcggc cgcccaaaga gagcgagaaa tactttagcc tgctgcgtgt agaagcggtc
     2161 aacggcatgg atccggaggc cgcgcggcgt cgcccgaact tcgacaacct caccccgatt
     2221 ttcccgctcg aactgatcga tctggagacc gagccgaata tcctctcgac gcgcctcctc
     2281 aacatggtgg ccccgatcgg ccgcgggcag cgcggactga tcgtttcacc gcccaaggcc
     2341 ggcaagacgc tgctgctgaa agcgatcgcc aacggcatca ccaccaacta cgccgacgtg
     2401 cacctcatcg tctgcctgat cggcgagcgg ccggaggaag tgaccgatat gaagcggtcg
     2461 gtcgccggtg aagtggtcag ttcgacgttc gacgagccgg tcgaggatca caccaaggtc
     2521 gcggagatga cgttggagcg ggccaagcgg ctggtcgaat gcggccgcga cgtggtgatc
     2581 ctgctcgact cgatcacgcg gctggcgcgc gcgtataacc tggccgtgcc gccgagcggg
     2641 cgcacgctct ccggcggtat cgacccggtg gcgctctatc cgcccaagcg cttcttcggc
     2701 gcggcgcgca atatcgaggg cggcggcagc ctgaccatcg tggcgacctg cctcatcgac
     2761 accggctccc gcatggacga tgtcatctac gaggagttca agggaaccgg caacatggag
     2821 ctgcacctcg accgcaagct ggccgagcgg cgcatctacc cctcgatcga catccagcgc
     2881 tcgggcaccc ggcgcgagga gctgctgctc gaagaccagg tgctgcgcca ggtctggacg
     2941 atgcggcgca tggtctcgat gctgggcggc accgagggca ccgagctggt gctgggtcgc
     3001 atggccaaga ccggcagcaa cgccgagttc ctggcgacgc tcaataagga tctctagccg
     3061 ggtctgcctg atccccaatg tgcgtgacgt tttgaggggc cgccatcgcg gcccctcttt
     3121 ccgtgtgttg cgcgcgcgaa tgaggcgcgg tggcccacga ctcggttgcc ggccgggcga
     3181 ggacggctcc cgtgcccgtc gaagaccgtc atgccgtgcc ggccaggatg tcgctggagc
     3241 gccagacgcg gtcgaggatg gtgctgatct cgtcgccttc ggccagccgg ggctggtagt
     3301 agtcctcgat catgatcggc acaaagcgca gccctgccag gcgtctgccg cggaagatga
     3361 gatgcatgat caggccctgt ttggtctcca gcgaccactc ttgatcgaag acgaagttcc
     3421 ccagcgagta gacaatcgga cggccgtggt aggtctcgac cccttgcacc caatgggggt
     3481 gtgagccgac gaccatgtcg gcgccggcgt cgatcgcctg gtgggcgata aggacctgac
     3541 cgtcggtcgg cgtcagcgtg tactcgaccc cccagtggat gaaggggatg acgagatcgg
     3601 ctttggggcg cacggcttgg atatcggcca ccaggttgtc cgccaccaat ggtgaagtcc
     3661 cggcgctgtc cccggtggcg ccataggtgt cgccggtgat gccgtcgaag ccgagcaggg
     3721 ccagcttgac ccctttcgct tgcaagatga ccggctggcg cgcctgggag agctgctctc
     3781 caatgccgaa gtggaggatc ttgcgcgcgt cgagcgcggc gagcgtgtcg cgcatgccgt
     3841 cggcaccgaa gttcatgctg tggttgttgg cctgactgac ggcatccacg ccggccagca
     3901 ccaggccgtc ggcggccgcc gcgcgcgtca tgaaggagaa ggtgtaggga tcggtcggcg
     3961 gggtgatggt gtctgagagg gtgcactcca ggtcgaggat ggtcaggtcg gcgcccttta
     4021 gttcgtcggc gaccttacgg aagggcgcgg cgaagtcgcc taggcgactg atgatggtgt
     4081 gcacggtgcg gcccaggatc acgtcgccgc tgaaggtgac ggtcaacggc gtcggggcgg
     4141 gtcggccgcc ggccagcgcg cccgactgcg cggcgagcca gggctttagt tgctcccaaa
     4201 caggcccgct gcccgtggca acgagcgcga gcgcgagcgg cgcccgcccg gtcggggttg
     4261 tctccgccag caagcgaacg tcatcgacgg gtggcgagcc ggcttgaaag tcgaccacgt
     4321 cgaggggcgc cagcgcgagc gcgccaggat gctgcggcag gagcgccagg aagccggcat
     4381 agtccggcac ctggatcgtc gcgggcgcag cgcggctgac ggcgacgcca ccgatcgcga
     4441 ccggctgcac cggagtcgcg gtcggcacgc ccaccttcgc ccagtcggtg atcgatccac
     4501 cgagcagatc gtcgagttgt tggcgcgtga gaccgggtag ccacagccgg ggcgaggtaa
     4561 ccggcgccag cggctgactt gccaggacga tggtgtcgtg atcggggtcg agcagcgagc
     4621 gcagcacgag gtcggcgtcg gtttgtcctt cgaccgggat cggctcgaca ccgcccacga
     4681 gcggcgcgct cgccagcagt tcaatcaccc gatcagcggt ctgtcgaaac gtgctggcga
     4741 ggctaggatc gagtaatacg cgaacagtca ccggaggtgg cgtcgctgtt ggagacggtg
     4801 gcagcggggt gggagagggc atcggggatg gcgccgcggt cgcgactccc gcgaccggtg
     4861 acgctgccgg cgcggttgag ggagacggta cggccgtggt ggcggcgggc ggctctggcc
     4921 cgcggcacgc ccagccgatg aacgcggaca tggcgagcag caagcgcctt cgcgagattg
     4981 gcaggcgatt catcttgact ccgttcgcgt gtgggtagat gatagcctcc aggatctcga
     5041 ccgacaagtg gttttgtgcc gcgtgcgcga cgccgcgtgc atcgatgaaa gtgggaacgc
     5101 tggtgaggag ccagggtggg caccgccgtg ccgactattc gcgcgcccgc cggatccacg
     5161 tgctccttgg gccgctgctg gcgctcgcgc tgctggcgtc cggcttagcc ccgcgctcct
     5221 cggcggcgtc agccccggcg aaggtctatt tcccagtcac cggccaccac gtctccgacc
     5281 aggtgctgac ctactggcgt gcgcacggcg acctgtcgat cttcggctat cccctgaccg
     5341 agccgatcca gcgcgatggc ctgacggtgc agtattttga acgcgcgcgt ctcgagctgc
     5401 atcctgagtt ggccgggacg ccctatgagg tcgagttgac gctgcttggc gatcagaccg
     5461 cggctgggca ccaggaagcg agcttccagc gcatgagcgc tgtaggggcc aacgactcga
     5521 aggatcgcgt ctacttttcg gcgactggtc actatctggc ctacggcttc aaatcgttct
     5581 gggacggcaa cggcggcctg gctatcttcg gctatccgat ctcggaggag ttctccgaga
     5641 agaacgcgga tgacggcaag acctacacgg tgcagtactt cgagcgggcg cgcttcgagt
     5701 accaccccga gttcaagggc acgcc
//
LOCUS       DASUBI010000192         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_500704, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000192 DASUBI010000000
VERSION     DASUBI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500704"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFU72_03790"
     CDS             complement(<1..663)
                     /locus_tag="VFU72_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEU5422640.1"
                     /translation="MTRDRARRANSVWANLPLRGKGLVVIAIPLVALLSMTLSLSLLM
                     RQDREATAWVTHSAEVQAEIQDVLALLVDAETGTRGYLLTGQNGFLQTYQLAQVALPE
                     HLARLTMLVQDNPDQSSRVERIVPLATQKLAVLSALQTSGTPGAALSPGAQQQLVSSN
                     ALMQTLRQELGAMQDDEARLLVARSARVKQLDARALVTTAISLLVGLVGGLLAMLLFT
                     DGV"
     gene            complement(822..2495)
                     /locus_tag="VFU72_03795"
     CDS             complement(822..2495)
                     /locus_tag="VFU72_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU5422641.1"
                     /translation="MAWRSLCTRCLLLIVPLLALLAPAGMGASAAPVDETAQALALIN
                     SYRAWAGVAPITRNADLDGSASAHARYYQLNYGDPSLSGMGLHAEQPGKPGFTGADMQ
                     DRVEAQGYGGWANENIGLSTDLLGSLDWFIGTINHRLTLIDPRYTDIGFGVINDGKVN
                     IEVIDVGAPSWSDTATPEWTAWPPDGTSGVGTSFDGETPDPFPGASYPVGYPITLKYF
                     GPGSVSYSSAKLTANGKDVPLFSATGSGWLTQQTFMIAASDPLQPGTTYTVTAGGSAN
                     GAPFTKTWSFQTATPTAHALADAHDFPPGLKSADPAVQNLWWSADGAVAEGWVQQSWL
                     WGPDSFAAKAEQYAGSPGNQRQVYYFDKSRMEISDPGADRSSQWFVTNGLLVREMITG
                     QVQSGPDSFTAARPAAIPLAGDEANNPAAPTYASLTHLTGAVPDQTGLAAINTLAQDG
                     TAGLDGSFAGVTKLGHYDATTGHNVAGVFWDWLSAQGWDPLYVMGRPLSEPYWVRTRV
                     KGVEQPVLVQAFERRVITYTPGNDPDWRVEMGNVGRHYYSWRYGQPPAG"
     gene            2658..2984
                     /locus_tag="VFU72_03800"
     CDS             2658..2984
                     /locus_tag="VFU72_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5422642.1"
                     /translation="MFVAILIAVFLIAVLAIWLTTHLVGLLITLLVAGFIGWLADMIV
                     PGKIPYGFPGAIAFGLIGSWLGGHFIHFGPHLAGLALIPGIIGAMILALAVQLYGTVA
                     SSTRLR"
     gene            complement(3000..3799)
                     /locus_tag="VFU72_03805"
                     /pseudo
     CDS             complement(3000..3799)
                     /locus_tag="VFU72_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(3842..>4217)
                     /locus_tag="VFU72_03810"
     CDS             complement(3842..>4217)
                     /locus_tag="VFU72_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5422643.1"
                     /translation="QQGQQGQMSQQTGMQGRGGQQQMMQRGPHTGKGPKRSDQHIDDE
                     VRRHLTQHGNLDASNIDVKVNNGEVTLTGTVDSRQSKRTAEQVVDSVPGVKDVTNQLK
                     IQQQQGQQGQTTQSAQQTTATH"
BASE COUNT          573 a         1430 c         1457 g          757 t
ORIGIN      
        1 aaccccgtcc gtaaagagca gcatcgccag cagcccgccg accagtccta cgagcaagct
       61 gatggcggtg gtgacgaggg cccgggcgtc gagctgcttc acccgggcgg agcgcgcgac
      121 gagcaagcgt gcttcgtcat cctgcatcgc ccccagctcc tggcggagcg tctgcatcag
      181 cgcgttgctg ctgacgagct gctgctgggc gccgggcgag agcgccgcgc ccggcgtgcc
      241 gctcgtctgc agcgcgctga gcaccgcgag cttctgggtc gccaggggca cgatacgctc
      301 gacgcgactg ctttgatcgg ggttgtcctg caccagcatc gtgagccggg ccagatgctc
      361 cggcagggca acctgggcca attgataggt ctgcagaaag ccattctgtc cggtcagcag
      421 gtagcccctg gtgcccgttt cggcgtccac caggagcgcg agcacgtcct ggatctcggc
      481 ctggacctcg gcggaatggg tgacccaggc ggtcgcctcg cgatcttgcc gcatcaacag
      541 gctgagcgag agggtcatcg acagcagggc caccaggggg atggcgatga ccaccagccc
      601 cttgccgcgc agcgggaggt tcgcccagac cgagttcgcc cgcctcgcgc gatcccgcgt
      661 catgccgtcc tcaccacttc cgcccgctgc ccgctgctcc tccgacggcg ggcagtatag
      721 gagggactct cgcggcctga caggagagag aggtaccggt ttgcgctcgt accgcgctgt
      781 tggagcgctg tcagccggga gctagcacgc gggcggccgg atcagcccgc cggcggctga
      841 ccgtagcgcc agctgtagta gtgccggcca acgttgccca tctcgacgcg ccagtcgggg
      901 tcattgccgg gcgtgtaggt gatcacccgg cgctcgaagg cctgcaccag caccggctgc
      961 tcgacgccct tgacgcgcgt gcgcacccag tagggctcgc tcagcgggcg gcccatgacg
     1021 tagagcggat cccagccctg cgcgctcagc cagtcccaga agacgccggc gacgttgtgg
     1081 ccggtggtgg cgtcgtagtg gccgagtttg gtcacgcccg cgaaggagcc gtcgagtccg
     1141 gccgtgccgt cttgggccag cgtgttgatc gccgccaggc ccgtctgatc ggggacggcg
     1201 cccgtcaggt gcgtcaggct ggcgtaggtc ggggcggcgg gattattcgc ttcgtctccg
     1261 gcgagcggga tggcggcggg cctggccgcc gtgaagctgt ctggcccgct ctgcacctgt
     1321 cccgtgatca tctcgcgcac cagcaggccg ttggtgacga accactggct ggaccgatcg
     1381 gcgccggggt cgctgatctc catgcggctc ttatcgaagt agtagacctg gcgctgattc
     1441 ccaggcgagc cggcgtactg ctcagccttc gcggcgaagc tgtccggccc ccagagccag
     1501 ctctgctgca cccagccttc ggccactgcc ccgtcggcgc tccaccacag gttctgcacc
     1561 gctgggtcgg ccgacttcag tccgggcggg aagtcgtgcg cgtccgcgag ggcgtgggcg
     1621 gtcggcgtcg ccgtctggaa gctccaggtc ttggtgaagg gagcgccgtt ggcgctgccg
     1681 ccggcggtta cggtgtaggt cgtgcccggc tgcagcgggt cgctcgccgc gatcatgaag
     1741 gtctgctgcg tcagccagcc gctgcccgtc gcgctgaaga gcggcacgtc tttaccgttg
     1801 gcggtcagtt tggcgctgct gtagctgacc gagccggggc cgaagtactt gagcgtgatc
     1861 gggtagccga ccgggtaact ggcgccgggg aagggatcgg gtgtctcgcc gtcgaagctg
     1921 gtgcccaccc cgctggtgcc gtcgggcggc caggcggtcc actccggcgt ggcggtgtcg
     1981 ctccagctcg gcgcgccgac gtcgatcacc tcgatgttga ctttgccgtc gttgatcacg
     2041 ccgaagccga tgtcggtgta gcgcgggtcg atcagtgtca gccggtggtt gatcgtgccg
     2101 atgaaccagt cgaggctccc cagcaagtcg gtgctgaggc cgatgttctc gttggcccag
     2161 ccgccgtagc cctgggcctc gacacggtcc tgcatatcgg cgccggtgaa gcccggcttg
     2221 cccggctgct cggcgtgcag ccccatgccg ctcaggctgg gatcgccgta gttgagctgg
     2281 tagtagcggg cgtgggcgct ggccgagccg tcgagatcgg cgttgcgcgt gatcggcgcc
     2341 accccggccc aggcgcggta gctgttgatc agggccagcg cctgggcggt ctcgtcgacc
     2401 ggcgcggccg aggcgcccat gccggccggc gcgagcagcg ccagcagcgg tacaatgagc
     2461 agcagacagc gggtacacag cgagcgccag gccatgaagc ctcttccctc cccgtcgagc
     2521 gcgtggcagt cccctgtccg atagtacaac gccgaacctg tccgctggta gcgctaacgg
     2581 tcgtggtcag ggcgggagcg gcgtgctata cagtaccgta gatacccgct cggttcggga
     2641 cggaaaggac ctctgagatg tttgtcgcca tcctcatcgc ggtgtttttg atcgccgtgc
     2701 tggccatctg gcttacgacg catctggtgg ggctgctgat cacgctgctg gtggccggct
     2761 tcatcggctg gctggccgac atgatcgtgc ccggcaagat cccctacggc ttccccggcg
     2821 ccatcgcctt cggcctgatc gggagctggc tcggcgggca cttcatccac ttcggcccgc
     2881 acctggccgg gctggcgctg atccccggca ttatcggcgc catgatcctg gcgctggccg
     2941 tacagctcta cggcaccgtc gccagcagca cccgtctgcg ctaggccgtg tctgcaaacc
     3001 tacaaccaga tcaggataca ggcgaaggtg agcagggcgt gatacgagtc ggcgagctta
     3061 tcatagcggg tggcgatcgc ccgattctgc ttcaagcgat tgagcagccg ttcgatgcgg
     3121 ttgcgctggc ggtaggcggc acggtcgaac tggcgctggc ggggctcgtc ggcccggcgc
     3181 gggatcaccg ccccgatccc ccggcggcgc aggtagcgcc gcacggtgcg gctgctgtag
     3241 cccttgtcgc ccaccacccg ctggggccgc agccgcggcc ggcctcgccc ccgccgccgg
     3301 accgccccct gctccaacaa ggtgggcaag gctttctgct cgttgcgctc gccacccgtc
     3361 aaacagaagg tgatgggctt gccgcggccc tcggcgcgca ggtggatctt ggtcgagaac
     3421 ccgccccgac tgcgtcccag cgcttggtcc ccttttttgc cgcccccgcc gcatgctggt
     3481 gggcccggac cacgctgccg tcgacaaaat ggatcgtcca gtcgagcttg ccctgcgcgt
     3541 cctcctcgac ctggagtgcg gccaggatcc ggtcccagat gccctgctgc tgccagcggc
     3601 ggaagcggct gtacaccgtc ttccagttcc cgtaccgctc cggcagatcg cgccacggcg
     3661 ctcccgtgcg tagccgccag cggatgccgt tgaggatctg ccgatggtcc agattgggcc
     3721 ggccaacctt cggtttgacc ggcgggagga gtggcgccag gcgctcccat tgctcattgg
     3781 tcagttcatg tcgtcgcata gcctaatgct acacgatcaa ccagcacttt gcagacacga
     3841 cctagtgcgt ggccgtggtc tgctgggccg actgggtcgt ctgcccctgc tgaccctgct
     3901 gctgctggat cttgagctgg ttggtaacgt ccttgacgcc cgggaccgaa tcgaccacct
     3961 gctccgccgt gcgcttggac tgacgcgaat cgacggtgcc ggtgagcgtc acctcgccgt
     4021 tgttgacctt gacgtcgatg ttcgaggcgt ccaggttgcc gtgctgggtc aggtggcgcc
     4081 ggacctcgtc gtcgatgtgc tggtcagagc gcttcgggcc cttgccggtg tgcgggccgc
     4141 gctgcatcat ctgctgctgc ccacccctgc cctgcatacc ggtctgctgg ctcatctggc
     4201 cctgctggcc ctgctgc
//
LOCUS       DASUBI010000193         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1112057, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000193 DASUBI010000000
VERSION     DASUBI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112057"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFU72_03815"
     CDS             complement(<1..638)
                     /locus_tag="VFU72_03815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5422644.1"
                     /translation="MATGIGDRPARAATRAPLDESRFRALVEHAPDLVAVLDSRAQIV
                     YIAPSVERLLGHAPDKLTGGSLFELTHPDDLEAALSAFQALIATPGPGAPVELRLRHA
                     DGSWRYVEALGHGALSIPGIEGVLLNARDISDRKQLEMQLAYQAFHDPLTGLPNRTVF
                     ADRLAHALSIAGRRGELVAVLFLDLDRFKTINDTLGHPVGDKLLTEVASRLRA"
     gene            917..2290
                     /locus_tag="VFU72_03820"
     CDS             917..2290
                     /locus_tag="VFU72_03820"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU5422645.1"
                     /translation="MVVEIIPAGRRTLAPSGEEILRRCQDEGVQFVNLQFTDVMGIVK
                     MVTIPVDVLPEVIAEGQWFDGSAVAGFARIAESDMFLMPDLTTFKIVPWERGRNTTAR
                     IICWVFRPNGDLFPGDPRAALLRQLERAARLGYVYHTGPELEFFLFRRSEGAIDPLPH
                     DRGSYFDFSTDLAAEVRKDMIRGLQELGITVEAGHHEIAAGQHEIDFEYGDGMLTADN
                     LVTSKYALKAIANQHGLYATFLPKPIEGIAGSGMHVHQSLSGLSSGANAFVDPDDAYG
                     LSEVAKHFIAGQLAHARGMSAVLAPLVNSYKRLVPEYEAPVYVSWARQNRSALIRIPM
                     LRAGRTEATRVELRCPDAAANPYLAFTAMLAAGLDGIERALPLPEPVEENLYHFTEED
                     LRRRNIPYLPATLGEALRELEQDEVVRAALGEHIFEKLIEAQYHEWDAYRRHVSQWEV
                     DRYLERY"
     gene            complement(2507..2779)
                     /locus_tag="VFU72_03825"
     CDS             complement(2507..2779)
                     /locus_tag="VFU72_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422646.1"
                     /translation="MAETHPIRLSQDEIKRQAKKYFEDELGMRLTRDELESLRFTGDK
                     GFVQIDIQPDTNNQCRVTLEHDGYEPRLQEFRRRLAKQATAETSAG"
     gene            2912..3337
                     /locus_tag="VFU72_03830"
     CDS             2912..3337
                     /locus_tag="VFU72_03830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5422647.1"
                     /translation="MTGKQGKGVQVSEQVDQRLRQAEIIWLTTVRGNGQPQPVPVWFL
                     WDGQTFLIYSQPDQAKLRNIRDNPRVALNLNSDPQGGTVTRLEGNAEIDKDAPLATVV
                     QPFVAKYAAAIQRIGMDPETFAREYSVAIRVTPTRVSAF"
     gene            complement(3346..>3572)
                     /locus_tag="VFU72_03835"
     CDS             complement(3346..>3572)
                     /locus_tag="VFU72_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5422648.1"
                     /translation="QSRVIPNREELEARVRELEARYAEGEVSLPPYWGGFRVAPESFE
                     FWQGRLNRLHDRLRYRPTADGAWIIERLAP"
BASE COUNT          534 a         1238 c         1235 g          565 t
ORIGIN      
        1 gcgcgcaggc gactcgcgac ttcggtcagc aacttatcgc cgaccggatg gcccagcgtg
       61 tcgttgatcg tcttgaagcg gtcgaggtcg aggaagagga ccgcgaccag ctcaccgcgc
      121 cgcccggcga tcgagagcgc atgcgcgagc cgatcggcga agacggtgcg gttgggcagg
      181 ccggtcaggg gatcgtgaaa cgcctgatag gcgagctgca tctcgagctg cttgcggtcg
      241 ctgatatcgc gcgcgttgag caggacaccc tcgatgccgg ggatgctcag ggcgccgtgc
      301 cccagcgctt ccacgtagcg ccagctcccg tcggcgtggc gcagccgcag ctcgaccggc
      361 gcgccggggc ctggcgtcgc gatcagtgcc tggaaggcac tcagcgcggc ctccaggtcg
      421 tcggggtgcg tcagctcgaa gagcgagccg ccagtcagct tgtccggcgc gtggccgagc
      481 aggcgctcga ccgaaggggc gatatagaca atttgtgccc gtgagtcaag cacggcgacc
      541 agatcgggcg catgctcaac cagggcgcga aagcggctct catcgagcgg tgcgcgcgtt
      601 gcggcgcgcg ccggcctgtc cccaatccct gttgccaccc tcgcctcacg ttcgctgggt
      661 tcgctccgcc gtgcggcgcc gcccggtctt catgccctca tgctagcgcg ccggcacgac
      721 cgcatcggat agcccatcgg tcatgggcga tggggcaccg gagtaccacc cgcggtcagg
      781 cccgcctgtg ctcccggcac aagcgcgcct gccgcgcttg ggcggcgcga ctcttggcgc
      841 gtgcgcgcgg cgcgcctagg cttggggcta ccggctgggg gtcggtggga tagccgtgcg
      901 cgaaggaggc gtggtcgtgg ttgtcgagat catcccggcg gggcggcgca cgctcgcacc
      961 ctcgggcgag gagatcctgc gccgttgcca ggacgagggc gtgcagttcg tcaacctgca
     1021 gttcaccgat gtgatgggca tcgtcaagat ggtgacgatc ccggtcgatg tgctgcccga
     1081 ggtgatcgcc gaggggcagt ggttcgacgg ctcggcggtc gccggcttcg cccgcatcgc
     1141 cgagagcgac atgttcctga tgcccgacct gacgaccttc aagatcgtac cctgggagcg
     1201 cggccgcaac accaccgcgc gcatcatctg ctgggtcttc cgccccaacg gcgatctctt
     1261 ccccggcgat ccgcgcgccg cgctgctgcg ccaactcgag cgcgccgccc ggctcggcta
     1321 cgtctaccac accgggccgg agctggagtt cttcctcttc cgccgcagcg aaggcgccat
     1381 cgacccgctg ccgcacgacc gcggcagcta cttcgatttc tcgaccgacc tggccgccga
     1441 ggtgcgcaag gacatgatcc gcgggctgca agagttgggg atcacggtgg aggcggggca
     1501 ccacgagatc gccgccggcc agcacgagat cgacttcgag tacggcgacg gcatgctgac
     1561 cgccgacaac ctggtcacct cgaagtacgc gttgaaggcg atcgccaacc agcacggcct
     1621 ctacgcgacc ttcctgccca agccgatcga ggggatcgcc ggctccggca tgcacgtcca
     1681 tcagagcctc tccgggctgt cgtcgggcgc caacgccttc gtcgatcccg acgacgccta
     1741 cgggctgtcg gaggtcgcca agcacttcat cgccgggcaa ttggcgcacg cccgtggcat
     1801 gagcgcggtg ctggcgccgc tggtcaactc ctacaagcgg ctggtgccgg agtatgaggc
     1861 gccggtctac gtcagttggg ccaggcagaa ccgctcggcg ctgatccgca tcccgatgct
     1921 gcgtgccggg cgcaccgagg cgacgcgcgt cgagctgcgc tgccccgacg ccgccgccaa
     1981 cccctacctg gccttcacgg cgatgctggc ggcgggcctc gacggcatcg agcgcgcgtt
     2041 gccgctgccg gagccggtgg aagagaacct gtatcacttc accgaagaag acctgcgccg
     2101 gcgcaatatc ccgtatctgc cggccacgct gggcgaggcg ctgcgcgagc tggagcagga
     2161 cgaggtcgtg cgcgcggcgc tcggggagca catctttgag aagctgatcg aggcgcagta
     2221 ccacgagtgg gacgcctacc gccgccacgt gagccagtgg gaggtcgacc gctatttgga
     2281 gcggtattga gggggacggg ccctcacccc cggccacggg gagccctcat tcccccgccc
     2341 cttctccggg gagccctcaa cccccaaccc cttctcccac tgcggtgggc gaaggggagc
     2401 acgtggcgac tacgacgtcg tgctccctcg cccccgcagg ggagagggcc ggggtgaggg
     2461 ctctccgtcg ggctgaggga tccccggcca gggggatgag gaccgtctac cccgcgctcg
     2521 tctccgccgt tgcctgcttg gccaggcggc ggcggaactc ctgcaaccgc ggctcgtagc
     2581 cgtcgtgctc gagcgtcacg cggcactggt tgttggtgtc gggctggatg tcgatctgga
     2641 cgaagccctt gtcgccggtg aagcgcaggc tctccagttc gtcgcgcgtc agccgcatgc
     2701 ccaactcatc ttcgaagtac ttcttcgcct gtcgcttgat ctcatcctgg ctgaggcgga
     2761 tcgggtgtgt ctcggccatc tcacgctcct ttccgtggct cgatcgcgtg ccgggccacg
     2821 tctccaccct cactctctca cgtcgcgtgc cggcaggtgg ccggacgtgg tagcctgagc
     2881 cgcggttcgc gcgcacggct aaggagcaaa catgaccggc aagcagggca agggtgttca
     2941 ggtgagcgag caggtcgatc agcggctgcg gcaggcggag atcatctggc tgacgaccgt
     3001 gcgcggcaac gggcagccgc agccggtgcc ggtctggttc ctttgggatg ggcagacgtt
     3061 cctgatctac tcccagcccg atcaggccaa gctgcgcaac atccgcgaca atccgcgggt
     3121 ggcgctcaat ctcaactccg atccgcaggg cggcacggtg acgcggctgg aaggcaacgc
     3181 ggagatcgac aaggacgcgc cgctggcgac cgtcgtgcag cctttcgtcg ccaagtacgc
     3241 cgccgcgatt cagcgcatcg ggatggaccc ggagaccttc gcccgcgagt attcggtcgc
     3301 catccgcgtc accccgacgc gcgtgtcggc cttctgaccg gcggctcagg gcgccaggcg
     3361 ctcgatgatc caggcgccgt cggcggtcgg gcggtagcgc aggcggtcgt gcagccgatt
     3421 gaggcgcccc tgccagaact cgaacgactc cggcgccacg cggaagccgc cccagtaggg
     3481 cggcagcgac acctcgccct cggcgtagcg cgcctccagc tcgcgcacgc gcgcctccag
     3541 ctcctcgcga ttcgggatca cccggctctg gt
//
LOCUS       DASUBI010000194         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1302663, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000194 DASUBI010000000
VERSION     DASUBI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302663"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFU72_03840"
     CDS             complement(<1..256)
                     /locus_tag="VFU72_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEU5422649.1"
                     /translation="MSTTTTIGDIVRGDREVFPPFLYAAYRSTVLRSPRLPLIEVPLT
                     LSELTGPGPAISAVTPADADLTSNAATGAEAIGQRIIVTGR"
     gene            complement(296..688)
                     /gene="pcaC"
                     /locus_tag="VFU72_03845"
     CDS             complement(296..688)
                     /gene="pcaC"
                     /locus_tag="VFU72_03845"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEU5422650.1"
                     /translation="MTESRYDEGLQVRRAVLGEAHVARALERQTAFDADFQRYITEAA
                     WGGVWARPGLDRRTRSLLTIVMLAALGRSEELALHLRASRNTGVETGEIAEALLQVAV
                     YAGVPAANTAFRIAKEVLGISPQSGTTE"
     gene            complement(1002..2180)
                     /locus_tag="VFU72_03850"
     CDS             complement(1002..2180)
                     /locus_tag="VFU72_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEU5422651.1"
                     /translation="MRTQVAIIGAGPAGLVLAHLLARAGIEAVVLEDRERGYVEQRVR
                     AGVLEQGPVDLLDELGLAARLHREGLVHRGIELRFEGKGHRIPLSDLTGGRAITIYGQ
                     QEVVKDLIQARLEAGGEILFEVSDVTLHGLDSDAPVVRFRHQGEARELRCDIVAGCDG
                     FHGVSRGAIPAGVLTVYENEYPFAWLGILAAVPPSTDELIYASHERGFALHSLRSPEI
                     SRLYLQVEPDEQIEEWPDARIWDELQARFATGDGWTLQRGPVLEKGITAMRSFVAEPM
                     QYGRLYLAGDAAHIVPATGAKGLNLAVADVRVLAEALAAWFHHGRTDLLDAYSTTALR
                     RVWRAQHFSWWMTSMLHRLHGPDEVFQRRLQLSQLHYTCTSPAAATSLAENYVGLERI
                     "
     gene            complement(2215..3306)
                     /locus_tag="VFU72_03855"
     CDS             complement(2215..3306)
                     /locus_tag="VFU72_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU5422652.1"
                     /translation="MGIWRSDVVGSLLRPGYLKEARKRHEAGELDEAAFKRVEDRAVD
                     AAVALQEGVGLDVITDGEMRRYAFFGHLIDAVEGFDKLGGWAIPFRDEHGNELVYPRP
                     VVVSRLQRRRPLAGEEFAYLRGRGDRPVKVTLISAQQAAAYYDPEKSKGAYPTIDAYL
                     ADVVDILRAEVAELIRLGCGYIQIDAPQYAALLDPQIREGYRQRGNDPDKLLARCIEL
                     DNAVIGEHPGVVFGIHLCRGNNQSRFYASGSYEPVAQVFERTRFQRFLLEYDDERSGD
                     FAPLRHVPDDRVVVLGLVTTKKPELESKEALKQRIHEAAQYIPLERLALSPQCGFAST
                     EEGNRVTPGDQEAKLWLVAETAREVWGNA"
BASE COUNT          526 a         1283 c         1311 g          567 t
ORIGIN      
        1 cccggccggt gacgatgatg cgctggccga tggcctcagc gccggtggcg gcgttgctgg
       61 tcaggtcggc gtccgcgggc gtgaccgcgc tgatggccgg accgggaccg gtcagctccg
      121 agagcgtcaa tggcacctcg atcagcggca gccggggcga gcgcagcacg gtcgagcggt
      181 aggccgcgta gaggaacggc gggaagacct cgcggtcccc gcggacgatg tcgccgatcg
      241 tggtcgtcgt gctcaccagt actctctcct tcgttgggca ggcaccggat gaagcctact
      301 ccgttgtccc gctctggggc gagatgccga gcacctcttt ggcgatgcgg aaggcggtgt
      361 tggccgccgg caccccggcg tagaccgcca cctgcagcag cgcctcggcg atctcgccgg
      421 tctcgacgcc ggtgttgcgg ctggcgcgca gatgcagggc cagctcctcg ctgcggccga
      481 gcgcggccag catgacgatg gtcagcaggc tgcgcgtgcg gcggtcgaga ccggggcgcg
      541 cccagacgcc gccccaggct gcctcggtga tgtagcgctg gaagtcggcg tcgaaggccg
      601 tctgccgctc cagcgcccgc gccacgtgcg cctcgcccag caccgcccgc cggacctgca
      661 acccctcgtc atagcgcgat tcggtcacga gtgatccttt cgggcgggca gcgccattgg
      721 tggatggtgt cgcctttggt gtgggtttgg cgccgcgtgg cgcgcgggaa taaattcacc
      781 gcgctgagtc catcaagccc actgaagggg ctgagaacga ttgtcgccgc gatttcacta
      841 tcacgcaccc attgtgcggc atatcaacgg gttttccgcg ggcattccgt cgtcgcctac
      901 cgtaaaccca gtcccttcag tggacttcgc ggactcagcc cgagaattct attctcgggc
      961 gccgtgcgtc gccgaacgtt ctcggcagcg acagaccccc atcaaatccg ctccaacccg
     1021 acataattct cggccagcga ggtcgcggcg gcaggggagg tacaggtgta gtggagctgc
     1081 gagagctgca ggcggcgctg gaagacctcg tcggggccgt ggagacggtg cagcatcgag
     1141 gtcatccacc aggagaagtg ctgcgcgcgc cagacccggc gcagcgccgt ggtggagtag
     1201 gcatcgagca ggtcggtgcg cccgtggtgg aaccaggccg ccagcgcctc ggccagcacg
     1261 cgcacgtcgg ccaccgccag gttgagcccc ttggcgccgg tggccggcac gatgtgggcc
     1321 gcgtcgccgg ccaggtagag gcggccgtac tgcattggct cggccacgaa gctgcgcatg
     1381 gccgtaatgc ccttctccag taccgggccg cgctggagcg tccagccgtc gccggtggcg
     1441 aagcgcgcct gcagctcgtc ccagatgcgc gcgtcgggcc actcctcgat ctgctcgtcc
     1501 ggctcgacct gcaggtagag gcggctgatc tcgggcgagc gcaggctgtg cagcgcgaag
     1561 ccgcgctcgt gtgaggcgta gatcagctcg tcggtcgaag gcggaaccgc ggccaggatg
     1621 ccgagccagg caaaggggta ttcgttctcg tagacggtca gcacgccggc cgggatcgcg
     1681 ccgcggctga cgccgtggaa gccgtcgcag cccgcgacga tgtcgcagcg cagctcgcgc
     1741 gcctcgccct ggtggcggaa gcgcaccacc ggcgcgtccg agtcgaggcc gtgcaacgtc
     1801 acgtcggaga cctcgaagag gatctcgcca ccggcctcca gccgggcctg gatcagatcc
     1861 ttgaccacct cctgctggcc gtagatggtg atggcgcgcc cgccggtcag gtcgctgagc
     1921 gggatgcggt ggcctttgcc ctcgaagcgc aactcgatgc cgcggtgcac cagcccctcg
     1981 cggtgcaggc gcgccgcgag ccccagctcg tcgagcaggt cgaccgggcc ctgctccagc
     2041 accccggcgc gcacgcgctg ctcgacgtag ccgcgctcgc ggtcctccag cacgaccgcc
     2101 tcgatccccg cgcgcgccag caggtgcgcc agcaccagcc cggccgggcc ggcgccgatg
     2161 atcgccacct gggtccgcat cggccgaact ctccctccgc tgagcggttc cggctcaggc
     2221 gtttccccag acctcgcgtg ccgtctcggc caccagccag agcttggcct cctggtcgcc
     2281 tggggtcacg cggttgccct cctcggtgga ggcgaagccg cactgcgggc tgagcgccag
     2341 ccgctccagc gggatgtact gggcagcctc gtggatgcgc tgcttgagcg cctccttgct
     2401 ctccagctcc ggcttcttgg tggtcaccag cccgagcacc accacgcggt cgtccggcac
     2461 gtggcgcagc ggggcgaagt cgcccgagcg ctcgtcgtcg tactccagca ggaagcgctg
     2521 gaagcgggtg cgctcgaaga cctgcgccac cggctcgtag ctgccgctgg cgtagaagcg
     2581 gctctggttg ttgccgcggc agaggtggat gccgaagacc acaccggggt gctcgccgat
     2641 gacggcgttg tccagctcga tgcagcgcgc cagcagctta tcggggtcgt tgccgcgctg
     2701 gcggtagccc tcgcggatct gcgggtcgag cagcgcggcg tactgtgggg cgtcgatctg
     2761 gatgtagccg cagccgagcc ggatcagctc ggcgacctcg gcccgcagga tgtcgaccac
     2821 gtcggccagg taggcgtcga tagtggggta ggcgcccttg gacttctcgg gatcgtagta
     2881 ggcggcggct tgctgcgcgc tgatcagcgt gactttgacc ggacggtcgc cgcgaccgcg
     2941 cagataggcg aactcctcgc cggccagcgg gcggcggcgt tgcaggcgcg agacgaccac
     3001 cgggcgcgga tagacgagct cgttcccatg ctcgtcgcgg aaggggatgg cccagccccc
     3061 gagcttatcg aagccttcaa ccgcgtcgat caggtggccg aagaaggcgt agcggcgcat
     3121 ctcgccgtcg gtgatcacgt ccagcccgac gccctcctgc agggcgacgg cggcgtcgac
     3181 ggcgcgatcc tcgacccgct tgaaggcggc ctcgtccagc tcgcccgcct cgtgccgctt
     3241 ccgggcttcc ttgagatagc ccggccgcag gaggctgccg accacgtcac tgcgccagat
     3301 ccccatcgcg tcccgtcctt tcgttcagcc ggctcggggc gattgtagca ccggccctca
     3361 cccccggcag gagtgccctc acccccggcc cctctcccct gcgggggcga ggggaggtgg
     3421 cgaacgacaa ggaggggcgt aggggggctc ccctatagca acatcttttc gatgggcagc
     3481 gcccctctcc cgcgcacggg agaggggtcg ggggtgaggg aactccgtcc ggggtgaggg
     3541 cccgtcacgc caccgtctgc ccgccgtcgg ccaccagcag cgtgccggtg acgaagctgg
     3601 cctcatcgct ggccaggaag aggaccacgt tggccagctc ctccggtgcg gcgtagcggc
     3661 gcagcggaat gcgcgagacc gccgcgc
//
LOCUS       DASUBI010000195         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1316514, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000195 DASUBI010000000
VERSION     DASUBI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316514"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFU72_03860"
     CDS             <1..543
                     /locus_tag="VFU72_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422653.1"
                     /translation="SAFVAVLTDGTHVGAYVCDGQTTAAWFTGAQQGEAIALTNDQGL
                     RLSATLHQAADGLQLSRGTMHEPDGTEHDFAAERVPAHGRAGLYRAVVPAPVGHGATD
                     EPPTVGVIVLPSGAYRGAYYADETVTPVTDVRLGSSRLTAIVPGIGSVEATQVSTPAT
                     TEVPRWPGPTTPHHLRVRAG"
     gene            complement(1799..2503)
                     /locus_tag="VFU72_03865"
     CDS             complement(1799..2503)
                     /locus_tag="VFU72_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422654.1"
                     /translation="MSTPPVVVPLILLVDDDRTILLTLRLQLEADGFRVMSAEHGQRA
                     LALAQGELPHLAIVDLMMPGIDGFALARQLRHYADLPIIMLTAIDDPAQKIAGLTHFA
                     DDYVTKPFTYGELLARIRNLLARAWPQGRLVASTCRVDDALTIDFARHVIHAGQEQRS
                     LTPTEARLLHLLVKNRGQILPNELLLDRLWPEGNGTMSSLWEYIRRLRDKLDDPAEEP
                     RYIESERGVGYRFRAE"
     gene            complement(2500..>2918)
                     /locus_tag="VFU72_03870"
     CDS             complement(2500..>2918)
                     /locus_tag="VFU72_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422655.1"
                     /translation="LADAERVEDVLTNLLDNAVKYSPAGGAIEVTIDAPPGGPDADTV
                     PSLHVSVHDCGLGIPPEEHERIFERFYRLDRLMVERAGGAGLGLYLCRSYIEGMGGRI
                     WVESRPGHGSTFRFTLPTAPGDDSPPSRPVPEVATL"
BASE COUNT          510 a          963 c          986 g          459 t
ORIGIN      
        1 agtgcattcg ttgccgtgct cacggatggg actcatgtag gtgcgtacgt ctgtgatgga
       61 cagacgaccg ccgcgtggtt caccggcgca caacagggcg aggccatcgc gctgaccaat
      121 gaccagggcc tgcggctctc ggcaacgcta caccaggcgg ccgatgggct gcagctctcg
      181 cgtgggacca tgcacgaacc ggacggcacg gagcacgatt ttgccgcgga gcgtgtgccg
      241 gcgcacgggc gggcaggcct ctaccgcgcg gtggtaccag cgcccgtcgg ccatggagcc
      301 accgacgagc cgccaacggt gggggtcatc gtgctcccct ccggcgccta ccggggcgcc
      361 tattatgccg atgagaccgt gaccccggtt acggacgtgc gcctcggtag cagtcgcctg
      421 accgcaattg tccccggcat cgggagtgtc gaggcgacgc aggtgagcac acctgccact
      481 acagaggtgc cgaggtggcc tggccctacc acgcctcacc acctcagggt gcgtgctgga
      541 tgagcatggc accccgacga tggtgactgg agtgtggccg gctggtccgc gtcacccgcg
      601 tcggcggaca ccgaccgagc ggcaaacgct tcttcacgtc gccagctgcg tgctgcggca
      661 gcgcgaccag ccgtcctgca ggcccgcgag gcgccgtgac tgccggctcg gtgtctcggt
      721 atttgtacgg cgggaaacga cgggccgggt cgtcaacgcc ctggatcacg agccacctgc
      781 tccacgcgta cgcccgcatg cgtgtggcag tgacgtaagc ggacagcggc tgctggccag
      841 cgaagaaccg gttgatgtcg ccgtcgctca caggcgatgg gcacgaacat tgctggagcg
      901 ctgcacaact ggcacgatgg cgcggtgcgc cagcgtcgcc gcgatcaccc gcgcggcgac
      961 atgagccatc gacagccggg ctggcagagc tagcgacatc gcgagctggt caactgtcgg
     1021 agatcgtcga gggaatacgg ggctacttcg ggtaccgcac tccatcgttg cccgcttctc
     1081 agaagcgggc gaagacatga catggcctct cgtcccacat agcgcaactg gagcgaatcg
     1141 ccgccgaaga ggtacaggct accgcgcgaa tcctcaagga ggaggagcag cgagtcagcc
     1201 cgatcgtagc cgcgatagcg gatggtgtac cgatccgcgt aggactcgcg gtcaaagccg
     1261 agcgtacgag gatgagcgtc ttgcaccaat ggcacctcct atgcacgcca actctactcg
     1321 ctccgcctca atacccacct caagattcgc gccggcggta ggtcacacgc gctgatgtct
     1381 accgtggtgc agacctggca ccgcaaaacc agcgccagcc aaagcgacgc agttgcgccg
     1441 cacgcgaccg gaacggcgag cagtgcagtg cagtgcttgg cggccgacac gacggcgagc
     1501 gtcctaccat gcggcggcga tcagtccact cgtgtcggca gagaggtcag tcacgaacag
     1561 ccgtctccgt gccgaatgtc aagtcacctc aggtgatcca cgttcatatg gttcgcgtgt
     1621 gtaggcccaa tcaccggcct cgctcctggt acgatgacgt gcgtagtacc gtgcggcccg
     1681 gcccgacaac cgcgaagcga gcacgtgcac gctgttcagg tctgccgccc gcgctgcgaa
     1741 tgggccccgg tcgtttggca cgtcggctgt gacgatagcg cagggctcag tgatgctgtc
     1801 actctgcgcg gaagcgatag ccaactcccc gctcgctctc gatgtagcgc ggctcctcgg
     1861 cgggatcgtc gagtttgtcc cgcaggcggc ggatatattc ccagagtgaa ctcatcgtac
     1921 cgttgccctc cggccagagc cggtcgagga gcagttcgtt cggcagaatc tgcccgcgat
     1981 tcttcacgag caagtggagc aagcgcgcct cggtgggggt cagcgagcgc tgctcctgcc
     2041 cggcgtggat gacgtgccgg gcgaagtcga tcgtcagcgc gtcgtcgacg cggcacgtcg
     2101 acgcgaccag ccggccctgc ggccacgcgc gcgccagcag gttgcggatc cgcgccagca
     2161 actcgccata ggtgaagggc ttggtcacgt agtcgtcggc aaagtgcgtg agccccgcaa
     2221 tcttctgcgc cgggtcgtca atggccgtca gcataatgat cggcaggtcg gcgtagtgcc
     2281 ggagttgtcg ggccagcgcg aagccatcga tgccaggcat catcaggtcg acaattgcca
     2341 ggtggggtaa ctcgccctgc gccagcgcca gcgcgcgctg cccgtgctcc gcgctcatga
     2401 cgcggaagcc gtcggcttcc agctggagcc gcagcgtcag caggatggtg cgatcgtcgt
     2461 ccaccagcag gatcagcggc acgacaacgg gcggggtact catagtgtgg cgacctccgg
     2521 caccgggcgc gacggcgggg agtcgtcacc tggcgcggtc ggcagggtga agcggaaggt
     2581 gctgccgtgg cccggccgac tctcgaccca gatacgcccg cccatgcctt cgatatatga
     2641 gcggcagagg tagaggccaa ggccggcccc gccggcccgc tcgaccatca ggcgatcgag
     2701 ccggtagaaa cgctcgaaga tccgctcgtg ctcctcgggc gggatgccga gcccgcagtc
     2761 gtggaccgat acgtgcaaac tcggcaccgt gtcggcgtcg gggccgccgg gcggcgcgtc
     2821 gatcgtcacc tcgatcgccc cccccgcagg ggagtacttg acagcattgt cgagcaggtt
     2881 ggtcagcacg tcctcgacac gctccgcatc ggccagcg
//
LOCUS       DASUBI010000196         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_689504, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000196 DASUBI010000000
VERSION     DASUBI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689504"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFU72_03875"
     CDS             <1..202
                     /locus_tag="VFU72_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5422656.1"
                     /translation="VVREPEPTDGRAVLIRATPAGRQLLEEGRARRTAVLARQLAALT
                     PEELATLQQAAEILDRLTRARD"
     gene            complement(482..1903)
                     /locus_tag="VFU72_03880"
     CDS             complement(482..1903)
                     /locus_tag="VFU72_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422657.1"
                     /translation="MKIAVIGGSAAGVFAALLLARAGHDVLVLERDHLDPALDVEAAA
                     AAAFRPTAPQIVQPHTVLARCRELLQERLPDVYRGLLAAGVGEAPLAAQLPAALAGTA
                     ARPDDERLTRLRTRRSTFDWVLRRALLAQRGVTVCFRVRVSELLAVPGVPPHVTGVHT
                     GQEAIGADLVVDATGRRSPIDRWLDEIGARPAATWRAETGVTYHSRHYRPRPAAALPG
                     PPPSGHIDVLAEFGVSIASTDNGAMQMTVTSLATDHRFRAVRRPEVFTAVLRTMPTYA
                     AWLDVLDPISPVYPMTTLHNTLRRLIVAGAPVVTGLHAIGDAVCATNPTFGRGLSMAL
                     WGATDLVDTIAQYGDDWTGQALALDALVAEHVQPYYEEQAAIDAARLAMLRHTIAGAP
                     APELPPARADRVTYGQLRAAARFDPTAFRALQSLLGMLRRPDAVYADPQVVVATHAVL
                     RERGSGPAPAQPTRAELLAALAT"
BASE COUNT          352 a          754 c          810 g          331 t
ORIGIN      
        1 agtggtccgc gagccggagc ctaccgatgg gcgcgcggtg ctgattcgcg cgacgccggc
       61 tggccggcag ctgcttgaag aaggccgggc gcggcgcacg gcggtgctgg ctcgccagct
      121 ggcggcattg acgccggagg agctggcgac gctgcaacag gccgccgaga ttctcgaccg
      181 gctgacgcgc gcgcgggatt gatgagtggc cctcactctc acgcctcctc atggtctcct
      241 gtacacatat tgctcgtggc cctggcaggc catagatggg aggcagatcg aggggcatac
      301 cggtcgctgt gctcgcttgg ttcgcaccgc accgtaagac gaggaaatct tgtaagtcgg
      361 cgacgatgta ggttatcaga ccactccagt ggagatcgtt tgtgaagcgt gtctccacat
      421 cgccgaaggt gcgcgacagc cggtgtcgct taagcggtcg gcgctgcctg cgacgcgccg
      481 gctacgtcgc cagcgccgcg agcagttctg cgcgggtcgg ttgcgccggc gccggcccgc
      541 tcccacgctc gcggaggacc gcgtgtgtcg cgacgacgac ctgcgggtcg gcatagaccg
      601 cgtcgggacg gcggagcatc cccaagagac tttgcagggc gcgaaacgcc gtcgggtcga
      661 aacgagctgc cgcgcgtagc tgcccgtagg tgacccggtc ggcacgggca ggtggcagct
      721 ccggggccgg ggcgccagcg atcgtgtgcc gcagcatcgc cagccgtgcg gcgtcgatgg
      781 cagcctgttc ctcgtagtag ggctgcacat gctccgcgac cagcgcatcc agcgccagag
      841 cctgcccggt ccagtcgtcg ccgtactggg cgatggtgtc gaccaggtct gtcgcgcccc
      901 acagtgccat gctcaggccg cggccgaacg tcgggttggt cgcgcagacc gcgtcgccga
      961 tcgcgtgcag gcccgtcacc accggggcac cagcgacgat gagacggcgc agggtgttgt
     1021 gcagcgtggt catcggatac acagggctga tcgggtccag cacgtcgagc cacgccgcgt
     1081 aggtcggcat ggtccgcagt accgcagtga acacctccgg gcgccggacc gcgcggaagc
     1141 ggtggtccgt ggccagcgag gtcacggtca tttgcattgc cccgttgtcg gtgctggcga
     1201 tgctgacgcc gaactcggcg aggacgtcga tgtgccccga tggcggcggg ccgggcagcg
     1261 cagccgctgg gcgcggccgg tagtggcgac tgtgataggt tactccggtc tccgctcgcc
     1321 aggtggccgc cggccgcgcg ccgatctcgt cgagccagcg gtcgatcggc gagcgccgcc
     1381 cggtggcgtc gaccacgaga tccgcaccga tggcctcctg gccggtgtgt acgcccgtca
     1441 cgtgcggtgg cacgccgggc acggcgagca gctccgatac gcgcaccctg aagcacaccg
     1501 tcacgccacg ctgggcgagg agcgcacgcc gcagcaccca gtcgaacgtc gagcgccgcg
     1561 tccgcagccg cgtcaggcgc tcgtcatccg gccgagccgc cgtgccggcc agcgccgccg
     1621 gcagctgcgc cgcgagcggc gcctcgccca cgccggcggc gagcaacccg cggtacacgt
     1681 cgggcagccg ctcttgcagg agctcgcggc acctagccag gacggtatgg ggctgcacga
     1741 tctgcggtgc cgtgggacgg aacgccgccg cagcggcggc ctccacatcg agcgcgggat
     1801 ccaggtggtc tcgttcgagc accaggacgt cgtgcccggc ccgcgccagc agcagtgcgg
     1861 cgaacacccc cgcggcgctc ccgccgatga cggcaatctt cattgccccg ccttcccgtc
     1921 gctgcggtga acacacctct gctcgacgct ggtcgggaaa tcgctggtac cggacaccac
     1981 gcaccatgga attgagtgat acacgaaata tagcgcaagt tgccagcggc agtgcgcctc
     2041 agcgccgggc gtggcatacc aggcgtgctc ggcgtagggc cagacagcga cgggagaggt
     2101 cgcggagtga gggcaatcgc gcgagtgtta ccacgctacc tactcgtccg tagatagaca
     2161 tggtctggcg ggcgtaagac gctccgggct acatgattgc gggacacgca cacggcgtcg
     2221 tgtcgggaat ggatggaaaa gcaacga
//
LOCUS       DASUBI010000197         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_772003, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000197 DASUBI010000000
VERSION     DASUBI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772003"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFU72_03885"
     CDS             complement(<1..939)
                     /locus_tag="VFU72_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5422658.1"
                     /translation="MLRTLIVGFSRAGMGLHWQVLRRMREAEPDSGLLSELPVMVYDL
                     RDVRERAARSGVVVASSLRRARAELEPDETVIHLCTPPTARLEILRELAELGFRHILV
                     EKPLAADRDTLRRIDELRRTHGLQLMVVAHWLDSSLTRNLRELLRSGELGSLRSISVL
                     QTKPRHTRTLAARGHPTAFEVEAPHSLGVVLSLAGDAEVATATAGDMHVGEQVVPLMG
                     SAHLVLRHHDGTRTEIFSDLTSPVRERRITLRLTRGLVVGHYSAGQDDRYAHLWVRAD
                     GREWRKLLPDDALMAFLSRAYRQFTVGEAPEAELELN"
     gene            complement(1002..>2089)
                     /locus_tag="VFU72_03890"
     CDS             complement(1002..>2089)
                     /locus_tag="VFU72_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5422659.1"
                     /translation="SARKVYEMTTPIPAQATATPSELRGQVLERVEDHLGSFIDFERE
                     RWSTVAARAATPIDAIASLIRAGGKRLRPAFCASGFLAAGGDPGDAMIVEVSAALELL
                     HAFALIQDDVLDNSPLRRGATTVHVRCADEHRLHGWRGEARRYGEGVAVLAGDLAFAY
                     ADRLMSGLPPRAREVWDELRTELIVGQYLDIAAAAEFLVDPALCRWIALCKSGRYTIY
                     RPLALGAAIAGRHDLIPMLAAYGLAVGEAFQLRDDLIGAFGDSKVTGKPAGLDFDQQK
                     MTLMLALAVQRDERVRRLVAAGGEGSWDAVALRPLLIEIGVRDEVERSIAQLVERASA
                     ALAEAAIDRGWREELASMALQVAYRDR"
BASE COUNT          305 a          769 c          735 g          280 t
ORIGIN      
        1 gttgagctcg agctctgcct cgggggcctc tccaacggtg aactgccggt aggcgcgcga
       61 caggaacgcc atgagcgcgt cgtcgggcag aagcttgcgc cactcgcggc cgtcggcgcg
      121 cacccagaga tgcgcgtagc ggtcgtcctg gccggcggag tagtggccga ccaccaggcc
      181 gcgcgtgagc cgcagcgtga tccggcgctc gcgcaccggc gaggtcaggt cggagaagat
      241 ctccgtgcgg gtgccatcgt ggtggcgcag gaccaggtgc gcgctcccca tcaaggggac
      301 cacctgctcg ccgacgtgca tatcgccggc ggtcgcggtc gccacctccg cgtcccctgc
      361 cagcgacagc accacgccga ggctgtgcgg ggcttcgacc tcgaacgcgg tggggtgacc
      421 acgggccgcc agcgtccgcg tgtgccgcgg cttggtctgc agcaccgaga tggaccgcag
      481 actgccgagc tcgccggagc gcagcagctc cctgaggttg cgggtgagac tgctgtcgag
      541 ccagtgcgcc accaccatga gctggagccc gtgcgtgcgc cgcagctcgt cgatccgccg
      601 caaagtatcg cggtcggcgg ccagcggctt ctccaccagg atgtgccgga accccagctc
      661 cgccagctcg cgcaggatct ccaggcgggc cgtcggcggc gtgcagaggt ggatcacggt
      721 ctcgtcgggc tccagctccg cgcgcgcacg gcgcagcgag gaggcgacga ccacgccgct
      781 tcgggccgcc cgctcccgca cgtcccggag gtcgtagacc atgacaggca gctccgagag
      841 caggcccgaa tccggctcgg cctcccgcat ccggcgcagc acctgccagt gcagtcccat
      901 gccggcgcgg ctgaagccga cgatgagggt cctcagcatc cgatcgcctt tcgcccgtgc
      961 tcatccggtc gccagggcgg gctcccaagc cctgcattcg ctcaccgatc ccgataggcc
     1021 acctggagcg ccatgctggc cagctcctcg cgccagccac ggtcgatcgc cgcctcggcg
     1081 agcgccgcgg acgcccgctc gaccagctgc gcgatggaac gctcgacctc gtcccgcacg
     1141 ccgatctcga tgagcagggg ccgcagcgcg acggcgtccc agctcccctc gccgccggcg
     1201 gcgacgagcc gccgcacgcg ctcgtcccgc tgcacggcca gcgccagcat gagggtcatc
     1261 ttctgctggt cgaagtccag ccccgccggc ttgccggtca ccttgctgtc gccgaaggcg
     1321 ccgatcaggt cgtcgcgaag ctggaacgct tcgcccacgg ccagcccgta ggccgcgagc
     1381 atcgggatca ggtcatgccg cccggcgatg gcggcgccca gggccagcgg ccggtagatc
     1441 gtgtagcggc cggacttgca cagcgcgatc cagcggcaca gcgccggatc caccaggaac
     1501 tcagccgccg cggcgatgtc caggtactgg ccgacgatca gctcggtgcg cagctcgtcc
     1561 cagacctcgc gggccctggg gggaaggccg gacatcagcc ggtcggcata ggcgaaggcg
     1621 aggtcgccgg cgaggacggc aacgccctcc ccgtagcgcc gcgcctcgcc gcgccagccg
     1681 tgcagccgat gctcatcggc gcagcggacg tggacggtgg tggcgccgcg gcgcagcggc
     1741 gagttgtcca gcacgtcgtc ctggataagc gcgaaggcgt gcagcagctc cagcgcggcg
     1801 gagacctcga cgatcatcgc gtcgccgggg tcgccaccgg ccgcgaggaa cccgcttgcg
     1861 cagaaggccg gccgcagccg cttgccaccg gcgcggatca gcgaggcgat cgcgtcgatc
     1921 ggcgtggccg ccctggcggc gaccgtcgac cagcgctcgc gctcgaaatc gatgaagctg
     1981 cccagatggt cctcgacccg ttccaggacc tgcccacgca gctcgctcgg ggtggcggta
     2041 gcctgggcag ggattggcgt ggtcatttcg tataccttcc gtgcagatc
//
LOCUS       DASUBI010000198         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1331720, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000198 DASUBI010000000
VERSION     DASUBI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331720"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="groEL"
                     /locus_tag="VFU72_03895"
     CDS             <1..1042
                     /gene="groEL"
                     /locus_tag="VFU72_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5422660.1"
                     /translation="ISAYFVTNTERMVSEIEDPFVLITDKKISAVQDILPLLEKALSI
                     TKNLVLIAEDVDGEALATLVVNKLRGTLNVLAVKAPGFGDRRKEMLRDMAILTGGTVI
                     SEEIGRRLDSATVQDLGRARRVTADKDNTTIVEGRGDEDAIKGRIAEIRTQIETTTSD
                     FDREKLQERLAKLAGGVAVIKVGAATETEQKEKQHRVEDALSATRAAVEEGIVPGGGV
                     ALINAIKGLDDVKAEGDVKTGVLILRRALEEPLRGIAQNAGLDGAVVISNVRRQQAEG
                     NENVGYDVLGDEYGDMLQKGIIDPAKVTRSAVQNAASIAAMILTTDALISDKPEEKAA
                     MPAGGMPGGMDY"
     gene            1253..>2553
                     /locus_tag="VFU72_03900"
     CDS             1253..>2553
                     /locus_tag="VFU72_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422661.1"
                     /translation="MAEPISSTFSGRTLPRLAGRDREQAVLRDRLSATLAGHGSLVLV
                     GGEAGIGKTTLAEALCAEATSQGALVLVGHCYDLTETPPYGPWAEALAAVPRDAASPP
                     TPDLAGENQATGREALFAAVRDYLAARSTDQALVLLLEDLHWADPASLDLLRAVARGV
                     TGVPLLCVATYRSDELTRRHPLYQLLPVLVREARATRLDVRPLDAASLRLLVRHYRLP
                     ERDEARLTGYLVERAEGNPFFAGELLRTLEEEGALRPDGDAWHLGDLAAIGVPSLLRQ
                     VIDARLDRLSGDGRVLLAAGAIVGQEVSLALWQVTSGADDETLATVMEAAQAAHLLVP
                     TADGARFAHALIREALYEGTLPLRRRAWHLRCAEALLAAPAPDPDAVAHHLQRAGDPR
                     AAEWLVRAGLRARGAVAWLTAAERFAGAAALLAGDASRARG"
BASE COUNT          383 a          906 c          924 g          340 t
ORIGIN      
        1 catctcggcc tacttcgtca ccaacaccga gcggatggtg tcggagatcg aagacccgtt
       61 cgtcctgatc accgacaaga agatctcggc ggtgcaggac atcctgccgc tgctggagaa
      121 ggcgctttcg atcaccaaga atctggttct catcgccgag gacgtggacg gtgaggcgct
      181 ggcgaccctg gtcgtcaaca agctgcgcgg cacgctcaac gtgcttgcgg tcaaggcgcc
      241 gggcttcggc gaccgccgca aggaaatgct gcgcgacatg gcgatcctga ccggcggcac
      301 ggtgatctcc gaggagatcg gccggcggct ggatagcgcg acggtgcagg acctgggccg
      361 cgcccgccgc gtgaccgccg acaaggacaa caccaccatc gtcgagggcc ggggggacga
      421 ggacgcgatc aagggccgga tcgccgagat tcgcacccag attgagacga ccaccagcga
      481 cttcgaccgt gagaagctgc aagagcgcct ggccaagctg gccggcggcg tggcggtgat
      541 caaggtcggc gccgcgaccg agaccgagca gaaggagaag cagcaccgcg tcgaggacgc
      601 gctgtcggcg acccgcgcgg cggtcgagga aggcatcgtg ccgggcggcg gcgtggcgct
      661 gatcaacgcg atcaagggcc tcgatgacgt caaggccgag ggcgacgtca agaccggtgt
      721 gctgatcctg cgccgggcgc tggaggagcc gctgcgcggc atcgcgcaga acgccggcct
      781 cgatggcgcg gtcgtgattt ccaatgtccg ccgccagcag gcggagggca acgagaacgt
      841 cggctacgac gtgctcggcg acgagtatgg cgacatgctc cagaagggca tcattgaccc
      901 ggcgaaggtc acgcgctcgg cggtgcagaa cgcggcctcg atcgcggcga tgattctgac
      961 caccgacgcc ctcatctccg acaagccgga ggagaaggcg gcgatgccgg ccggcggtat
     1021 gccgggcggc atggactact aggccgaaac gccgcaagat agcggcttgc acgcgcgccg
     1081 gccggggcag gagttgcccc ggccggcgcg tcttcgtgct cgcccggcac agcacttacc
     1141 ctcgtgctac aacgcaccca agtgggtcgc gatccgcccg actcgactaa ctttgctata
     1201 ctgcgggaac agattcgcgc ggacggcgac gcccgcctgg cccaggtgtg ccatggccga
     1261 gccgatcagc tccaccttct cgggccgcac actcccgcgc ctcgccggcc gggaccgtga
     1321 gcaggcagtg ctgcgcgacc ggctctccgc cacgctggcc ggacatggca gcctggtgct
     1381 agtcggtggc gaggcgggca tcggcaagac caccctcgcc gaagcccttt gcgctgaggc
     1441 gacgtcgcag ggcgcgctgg tgctggtggg ccattgctat gacctgaccg agacgccgcc
     1501 gtatggcccc tgggctgagg ccctggcggc tgtcccgcgc gatgctgcat cgccgccaac
     1561 gcccgacctc gccggggaaa accaggcgac cggtcgcgag gcgctgttcg cggcggtgcg
     1621 cgactacctc gcggcgcgat ccaccgacca ggcgctcgtc ctgctgctgg aagacctaca
     1681 ctgggccgac cccgcctccc tcgacctcct gcgcgccgtg gcgcgcggtg tgaccggggt
     1741 gccgctcctc tgcgtcgcca cctaccggag cgacgaactc acgcgccgcc acccgctcta
     1801 ccagctgctc ccggtcctgg tgcgcgaggc gcgggcgacg cgcctggacg tgcggcccct
     1861 cgacgcggcg agcctgcggc tgctcgtgcg ccactaccgc ctgcccgagc gcgacgaggc
     1921 gcgactgacc ggctacctgg tcgagcgcgc ggagggcaac ccgttcttcg cgggcgaact
     1981 gctgcgcacg ttggaggagg agggcgcgct gcgcccggac ggcgacgcct ggcacctggg
     2041 cgacctcgcg gcaatcgggg tgccgtcgct cctgcgccag gtcatcgacg cacgcctcga
     2101 ccgactcagc ggggatggtc gcgtcctgct cgcggccggg gcgatcgtgg gtcaggaggt
     2161 ctccctggcg ctctggcagg tgaccagcgg cgcggacgac gagacgctgg cgacggtcat
     2221 ggaggcggcg caagcggcgc acctcctggt gccgaccgcc gacggcgccc gcttcgccca
     2281 tgcgttgatt cgcgaggcgc tctacgaggg cacgctgccg ctgcgccgcc gggcctggca
     2341 cctgcgctgc gccgaggcgc tcctcgccgc gcctgccccc gacccggacg cggtggccca
     2401 ccacctccag cgggcgggcg acccgcgcgc cgccgagtgg ctcgtccgcg ccgggctgcg
     2461 agcgcggggg gccgtcgcct ggctcaccgc cgccgagcgc ttcgcggggg ccgccgcctt
     2521 gctcgcggga gacgcatcgc gcgcgcgtgg ctg
//
LOCUS       DASUBI010000199         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_502041, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000199 DASUBI010000000
VERSION     DASUBI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502041"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1926
                     /locus_tag="VFU72_03905"
     CDS             145..1926
                     /locus_tag="VFU72_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEU5422662.1"
                     /translation="MSYQPANPIIVQSDKSILLEVNNPAYEAARDALARFAELEKSPE
                     HIHTYRITPLSLWNAAAAGYGADFIIGALERFGKYELPGNVRTDISDYVARYGRVKLV
                     MDDGRLRLVSDDQPLLVEIANHRHLAPFIVERDARGLLVDGARRGHLKQALVQYGYPA
                     EDLAGYVEGAPFEIALRPATLSAGEPFSLRDYQRDSVAVFHAGGAAQGGSGVIVMPCG
                     AGKTMVGLAAMAELGCHTLILTPSIVAARQWMHEIVERTTIPPEAVGEYSGEVKEIRP
                     ITVTTYQVLTYRRKRGGEFPHFALFNQGDWGLIIYDEVHLLPAPVFRITAEIQARRRL
                     GLTATLVREDHCEGDVFALIGPKKYDVPWKDLERQGWIASAECHEVRIKLPEELRLEY
                     ALTEEREKYRFAAENPAKLAVLDQLVAKHKDDHVLIIGQYLDQLDSVAARYDAPLITG
                     KTATREREKLYEAFRQGEIRLMVVSKVANFSIDLPDASVAIQISGTFGSRQEEAQRLG
                     RILRPKSDGTLAHFYSLVTRETRDQEFAANRQKFLTEQGYSYEILYEDEVADFQPRAV
                     PEPRLLSGVDEVQSWGWSNRELTGTNE"
     gene            1930..>2410
                     /locus_tag="VFU72_03910"
     CDS             1930..>2410
                     /locus_tag="VFU72_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422663.1"
                     /translation="MLQQQPTFPVSFTEADVRRLCGTGLFERADRLQRAGHVLGPTFD
                     GATLAASVRGTWRRVDQASVGARAGQLEPACGCATPGFCQHAGALLLHWLRAPGAFQI
                     VLPGPEPGAPQALRRAAADSVTLGAISLPEGEPDELLTEALERDTNNHLRQIARARGV
                     "
BASE COUNT          379 a          857 c          819 g          355 t
ORIGIN      
        1 tcgttcccac ggctcccggt ctggggactg cttccccgcc agaaggtcgt ccatttcccc
       61 agacagtccc cagtccagcg ggaatcacga ggtctgcgcc gataggggct gattttcgcc
      121 gcatattggc gtataattga ctccatgagc tatcaacctg ccaacccgat catcgtgcag
      181 agcgacaagt ccatcctcct ggaggtcaac aacccggcct acgaggcggc gcgcgacgcg
      241 ctggcgcgct tcgccgaact cgaaaagagc cccgagcaca tccacaccta ccgcatcacc
      301 ccgctctcgc tctggaacgc ggccgccgct ggctacggcg ccgatttcat catcggcgcg
      361 ctggagcgct tcgggaagta cgagctgccc ggcaacgtgc gcaccgacat cagcgactac
      421 gtcgcgcgct acggccgtgt caagctggtc atggatgacg gccgcctgcg gctcgtctcc
      481 gacgatcagc cgctgctggt cgagatcgcc aaccaccgcc acctggcccc cttcatcgtc
      541 gagcgggacg cgcgcgggct gctggtcgac ggcgcgcggc gcggccacct caagcaggcg
      601 ctggtgcagt acggctaccc ggccgaggac ctggccggct acgtcgaggg cgcgcccttc
      661 gagatcgcgc tgcgcccggc caccctcagc gcgggcgagc ccttctccct gcgcgactac
      721 cagcgcgact cggtggcggt cttccacgcc ggcggcgcgg cccagggcgg cagcggtgtg
      781 atcgtgatgc cctgcggcgc cggcaagacg atggttggcc tggcggcgat ggccgagctg
      841 ggctgccaca cgctgatcct gaccccctcg atcgtggccg cgcgccagtg gatgcacgag
      901 atcgtcgagc gcaccaccat tccgcccgag gcggtcggcg agtacagtgg cgaggtcaag
      961 gagatccgcc cgatcacggt gaccacctat caggtgctga cctaccggcg caagcgcggc
     1021 ggcgagttcc cgcacttcgc cctgtttaac cagggcgact gggggctgat catctacgac
     1081 gaggtccacc tgctgcccgc gccggtcttc cgcatcacgg ccgagattca ggcgcggcgg
     1141 cggctggggc tgaccgccac gctggtgcgt gaggatcact gcgagggcga tgtcttcgcc
     1201 ctgatcgggc ccaagaagta cgacgtgccc tggaaggatc tggagcgcca gggctggatc
     1261 gccagcgccg agtgccacga ggtgcgcatc aagctccccg aggagttgcg gctggagtac
     1321 gcgctgaccg aggagcggga gaagtaccgc ttcgccgccg agaacccggc caagctggcg
     1381 gtgctcgacc agttggtggc caagcacaag gacgaccacg tgctgatcat cgggcagtat
     1441 ctcgaccagc tcgacagcgt ggccgcgcgc tacgacgcgc cgctgatcac cggcaagacg
     1501 gccacccgcg agcgcgagaa gctctacgag gccttccgcc agggcgagat ccggctgatg
     1561 gtcgtctcga aggtcgccaa cttctcgatc gacctgcccg acgccagcgt cgccatccag
     1621 atctccggca ccttcggctc gcgccaggaa gaggcgcagc ggctcgggcg catcctgcgg
     1681 cccaagagcg acggcacgct agcccacttc tactcgctgg tgacgcgcga gacgcgcgac
     1741 caggagttcg ccgccaaccg gcagaagttc ctcaccgagc aggggtatag ctacgagatc
     1801 ctctacgagg acgaggtggc ggacttccag ccgcgcgccg tgcccgagcc gcgcctgctc
     1861 tcgggcgtgg acgaggtgca gtcgtggggc tggtcgaacc gcgagctgac cgggacgaac
     1921 gagtaggcga tgctccagca gcagcccacc tttcccgtca gcttcacgga ggcggatgtg
     1981 cgccgcctct gcggcaccgg cctcttcgaa cgcgccgatc gcctgcagcg cgcgggccat
     2041 gtcctcggcc caaccttcga cggcgctacg ctggccgcca gcgtgcgcgg cacctggcgg
     2101 cgcgtcgacc aggcgtcggt cggcgcccgg gccgggcagc ttgagccggc ctgcggctgc
     2161 gccacgccgg ggttctgcca acacgccggg gcgctgctgc tccattggct gcgcgcccct
     2221 ggggcgtttc agatcgtgct gcctggcccc gagccgggag cgccccaagc gttgcgccgg
     2281 gcggcggcgg actcggtcac gctcggcgcg atttcgctgc cggaggggga gcccgacgag
     2341 ctgctgaccg aggcgctgga gcgcgatacc aacaaccacc tgcggcagat cgcgcgcgcg
     2401 cgcggcgtgt
//
LOCUS       DASUBI010000200         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_785494, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000200 DASUBI010000000
VERSION     DASUBI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785494"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1499
                     /locus_tag="VFU72_03915"
     CDS             189..1499
                     /locus_tag="VFU72_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422664.1"
                     /translation="MNRARVGMGVIGRVRGAGEQTFASLAVRDFRVLWFGFMGSWMAM
                     QMQQVARGVLAYQLTGNAFSLGLVTLAMGLPRIFLSPLGGVVADRFAKRTVLLWTQCA
                     LGLIALWGALMVAAGWMTIDWLVVLGLLQGAAFALNMPARQAYIPQIVGRGDSLANAI
                     ALNTAGMNLTRVVGPAVAGLLIAVSFINIAGVFFLVALCYIWVWASIYRVEERGESKM
                     GRRAGVRGSLTDGFSYIAQHRTLLVLISLGFVPLAIGMPYLSLMPVIALGNLKIGSGG
                     LGFLLTIAGVGALCGTLAIAYLARYEHKRQLQLVLGVLFGASLLGFAFFVLRGELLLA
                     LPFLFVTGMTGDAYQALNSTLIMMNTDEAVYGRVMGVYMVAQSIRPITVLPISAIADA
                     IGTPLTLMFSGGFCAAFVGGLAAFYPGYRRINEPQPAPEPVLAD"
     gene            1811..2791
                     /locus_tag="VFU72_03920"
     CDS             1811..2791
                     /locus_tag="VFU72_03920"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5422665.1"
                     /translation="MAALVRGRATIEQLILQFGVLIVALMVFAGELGVPFGIPIEVAL
                     LLVGAYTLHSAGALIGAIVLVVVADVLGTTLLYYLVRAGSQRVFARVLRKLGQDATML
                     DRWEARMRRYGARTVFAGRMLPMVRMYITIAAGVTRMPIGRFLLGEAPAAAIWSGAPL
                     LFGYFFRHDVRTLIGEYSSLSHAATLILPAVGPVVALIWWIQHGKTPRERLHRLRAVL
                     SITSTFVIIAYLAEAIDHFLSGHTGLSAPLTFLLPFLVVLAGISLTLLLIGIADLRSA
                     TRRITLPLPIIRLIAPEFRTTVVWFTLMVVAGIIIAGIELHYPTITLLYR"
BASE COUNT          373 a         1001 c          953 g          532 t
ORIGIN      
        1 gaggccatgc gcggggtcct ttcgcggtga aatactttga caatcgatat gcttgtagta
       61 gtatacgcgg cggcgcgggt cgccgtcccc cgctcaaccc gatctcgacg gcgcgggagc
      121 ggtgaatcgc cccaacgtga ccccaatctt agtgtacgcg gcggcagcga gcgagaagga
      181 tgcggcggtt gaatcgagca cgggtgggga tgggtgtcat cggacgggtg cgcggggcgg
      241 gcgagcagac ctttgcctcg ctggccgtgc gcgacttccg ggtcctctgg ttcggcttca
      301 tgggctcctg gatggcgatg cagatgcagc aggtggcgcg cggcgtgctc gcctaccagc
      361 tcaccggcaa cgctttctcc ctcggcctgg tgacgctggc catgggcctg ccgcgcatct
      421 tcctctcgcc gctcggcggc gtggtagccg atcgcttcgc caagcgcacc gttctcctct
      481 ggactcagtg cgccctcggt ttgatcgcgc tctggggcgc gctcatggtc gcggccggct
      541 ggatgacgat cgactggctg gtcgtgctcg ggctgctgca aggggcggcc ttcgcgctca
      601 acatgcccgc gcgccaggcc tacatccccc agatcgtcgg ccgcggcgac agcctggcca
      661 atgccatcgc actcaacacg gccggcatga acctaacgcg tgtggtcggc ccggcagtag
      721 ccgggctcct gatcgccgtc tcgttcatca acatcgccgg cgtcttcttc ctggtggcgc
      781 tgtgctacat ctgggtctgg gcctcgatct atcgcgtcga agagcgcggc gagtccaaga
      841 tggggcggcg cgctggggtg cgcggcagcc tgacggacgg cttcagctac atcgcgcagc
      901 accgcacgct gctggtgctg atcagcctgg gtttcgtgcc gctggcgatc ggcatgccct
      961 atctcagcct gatgccggtg atcgccctcg gcaatctgaa gatcggctcg ggtgggctgg
     1021 gcttcctgct gacgatcgcg ggggtcggcg cgctctgcgg cacgctggcg atcgcctacc
     1081 tggcgcgcta cgagcacaag cggcagctcc aactggtgct gggcgtcctc ttcggcgcct
     1141 cgctcctcgg ctttgccttc ttcgtgctgc ggggtgagct cctgctggcc cttcccttcc
     1201 tcttcgtcac cggcatgacc ggcgacgcct accaggcgct caacagcacg ctgatcatga
     1261 tgaacaccga cgaggcggtc tacggccggg tgatgggcgt ctacatggtg gcgcagtcga
     1321 ttcggccgat caccgtgctg ccgatcagcg ccatcgccga cgccatcggc acaccgctga
     1381 cgctgatgtt ctccggcggc ttctgcgccg ccttcgtcgg cggcctggcc gccttctacc
     1441 ccggctaccg tcgcatcaac gagccccagc ccgcgcccga gccggtgctg gccgactgag
     1501 caccggttcc cgtctcgccg accggcgtca tggcaggagc cggtgtggtg gagtacactc
     1561 gcggcgccgg tgtccgtgca tgggcgggcg gtgatgcgcc gcgctgccag cccgagtcac
     1621 cggccggcga ttcgcgcccg gcgtcgcgcg ctcgacagga gcgaccgacc gcgccggcgt
     1681 gccagggatg gtgacgaagg ttcacgatgg agccggaatc acggcaatcc gccgtccacg
     1741 ggccgcgccg gatttcccct ttggcgatga tggaccctcg cctgcgtaga tatggtccgg
     1801 gcggcacccc gtggctgccc tggttcgagg gagggcgacc atcgagcaac tgatcctgca
     1861 attcggcgtc ctgatcgtcg ccttgatggt cttcgcgggc gagctgggcg ttccctttgg
     1921 gatcccaatc gaggtcgcgc tgctcctggt cggcgcctac acgctgcact cggccggggc
     1981 gctgatcggc gcgatcgtgc tggtggtcgt ggccgacgtg ctcgggacca cgctcctcta
     2041 ctatctggta cgcgccggct cccagcgcgt cttcgcccgc gtgctgcgca agctggggca
     2101 ggacgccacc atgctcgacc gctgggaggc gcgcatgcgg cgctacggcg cccgcaccgt
     2161 cttcgccggc cgcatgctgc cgatggtgcg catgtacatc accatcgcgg ccggggtgac
     2221 ccgcatgccg atcggccgct tcctgcttgg ggaggcgccg gccgccgcca tctggtcggg
     2281 cgcgccgctc ctgttcggct acttcttccg ccatgacgtg cgcacgctga tcggagagta
     2341 cagcagcctc tcacacgcgg ccacgctgat cctgccggcg gtcggaccgg tggtggcgct
     2401 gatctggtgg attcagcacg gcaagacacc ccgggagcgc ctgcaccggc tgcgcgcggt
     2461 gctgagcatc acctccacct tcgtgatcat tgcctacctg gcggaggcga tcgaccactt
     2521 cctgagcggc cacaccggcc tcagcgcgcc gctgaccttc ctgctcccct tcctggtagt
     2581 gctggccggt atctcgctga cgcttctcct gatcggcatc gccgacttgc gcagcgccac
     2641 ccggcgcatc acgctgccgc tcccgatcat ccggctgatc gcgccggagt tccgcaccac
     2701 cgtcgtctgg ttcaccttga tggtggtggc cggcatcatc atcgccggca tcgagctcca
     2761 ctacccgacg atcacgctgc tctatcgata gaggagctcg cgctcgaagt catccgagcg
     2821 agccaggccc agcgtctcgc gcgcctcggc cacggcgta
//
LOCUS       DASUBI010000201         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_322154, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000201 DASUBI010000000
VERSION     DASUBI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322154"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFU72_03925"
     CDS             <1..804
                     /locus_tag="VFU72_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422666.1"
                     /translation="YYKNPHPHFKVLEVRQAMANSVDRKTVAEQLYGPGGSATGQTQN
                     TNKPYMLPDSDPAAQWKFDLKAAADLLDKAGAQKGGDGIRALNGRKMSWIYQTSVNEV
                     RQKNQEIVKQSLSTLGIDVQIKSVDASVFFSGDPGNNDTYSHFYTDLEMYTNGAGIFP
                     LNWYRRYLSANPDTDVAQKSNAWAGTNVSRYQSDAFNKLWQQAQTEIDPQKSIKLFQD
                     MQRQILTDVVEIGTVARNNVACVSNTLKGYNPTQWASDLWDVKNWTRTS"
     gene            875..1273
                     /locus_tag="VFU72_03930"
     CDS             875..1273
                     /locus_tag="VFU72_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5422667.1"
                     /translation="MRAERVLETCLYAADLDAAERFYRDVLGLAVESKVAGRHVFFRC
                     GAAVFLVFNPEATRRGGGPVPAHGASGPGHVAFAARPEELPGWREQLARGGVPLEQEV
                     RWPGGGVSLYFRDPAGNSIELATPGIWGQE"
     gene            1499..3451
                     /locus_tag="VFU72_03935"
     CDS             1499..3451
                     /locus_tag="VFU72_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5422668.1"
                     /translation="MSQDPRFDDLMRAAREGRLNRRALLKRAAVLGLSAPAIAALLAA
                     CGGSSSTSTSAAGTSAPTTSGGSAATAAPTTAAAATKAPTTAAASPVTNPGAATAETA
                     SGPAGGKGDLKLLWWQAPTIINPHIAQGTKDYDASRIVYEPLADFDANSKMVPMLAAE
                     IPSLDNGMVAKDGLSVTWKLRQGVKWHDGQPFTANDVKFTFDLVSDPKAAATTSGVYL
                     NVKSVDVVDDNTAKVNFKQPEPAWYYSFPGANGMILPQHVFKDYTGANLRNAPANLKP
                     IGTGAFKLTDFKPGDVGTYEINKDYWDKGKPHFDSITMKGGGDAPSAARAALQTGESD
                     WSWNIQVSPTVLKSMETAGKGKVVNWPGSGTERLEINWSDPQTEVNGEKSYYKNPHPH
                     FKVPEVRQALNNSIDRKTIAEQLYGSGGAPTGQTQNENTPYMLPASDPAAQWKFDLKA
                     AADLLDKAGAQKGGDGIRTLNGRKMSWVYQTSVNEVRQKHQEIVKQALGSIGVEVQIK
                     SIDASVYFSGDAGNPDTTGHFYADLEMHTNGCGIFPLNWYKRYLSANPDTDIPQKSNS
                     WAPSNIFRYQSDEFNTMWAQAQTELDPQKSIALFQNMQKLVLKDVVEIGQIARANVAC
                     VSNRLKGYNPTQWASDVWDIKNWTTA"
     gene            4027..>4952
                     /locus_tag="VFU72_03940"
     CDS             4027..>4952
                     /locus_tag="VFU72_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422669.1"
                     /translation="MNQDPRYDELLRAVRAGRMSRRGLLKRAAVLGLAAPAISALLAA
                     CGGGSNSSTTTGGTTTSGTGGTSTTGGTGAASTPAAKVPASPVFNPSAATPQASGATG
                     QRGGKGELKLLYWQAPTIINPHLAQGTKDFQASRVCLEPLADFDANTKMVPFLATEIP
                     SLDNGSVAKDGKSVTWKLRQGVTWHDGQPFTANDVKFTFDYVSDQATAATTYGSYVNV
                     DSVDAVDDHTVKVNFKQPEPAWYQPFTGENGMILPQHIFKDYKGATARQAPANLKPIG
                     TGPYQVTDFKPGDVVVYAINKNYWQDGAPHFDG"
BASE COUNT          965 a         1748 c         1549 g          690 t
ORIGIN      
        1 tactacaaga atcctcaccc gcacttcaag gtgctcgagg tgcgccaggc gatggccaac
       61 tcggtcgacc gcaagacggt tgccgagcag ctttacggcc ccggcggctc ggccaccggc
      121 cagacccaaa acaccaacaa gccgtacatg ctgccggact ccgacccggc ggcccaatgg
      181 aagttcgatc tcaaggccgc ggccgacctg ctcgacaagg ccggcgcgca gaagggcggc
      241 gacggtatcc gcgccctcaa tgggcgcaag atgtcctgga tctaccagac ctcggtcaac
      301 gaggtgcgcc agaagaacca ggagatcgtc aagcagtcgc tctccacgct gggcatcgac
      361 gtccagatca agtcggtcga cgcctcggtc ttcttctccg gcgaccccgg caacaacgac
      421 acctacagcc acttctatac cgacctggag atgtacacca acggcgccgg tatcttcccg
      481 ctcaactggt atcgccgcta cctctcggcc aatccggata ccgacgtcgc gcagaagtcg
      541 aacgcctggg ccggcaccaa cgtcagccgc taccagagcg acgccttcaa caagctctgg
      601 cagcaggcgc agaccgagat cgacccgcag aagtcgatca agctcttcca ggacatgcag
      661 cggcagatcc tgaccgacgt ggtcgagatc ggcaccgtgg cgcgcaacaa cgtggcctgc
      721 gtcagcaaca cgctaaaggg ctacaacccg acgcagtggg cctccgatct ctgggacgtc
      781 aagaactgga cgcgcacgtc ctagcgcggc cgcgagcgct tgtggtggcc ggatcgtgca
      841 cacgagccgg ccacgcggct actggagagt gatcatgcgc gctgagcggg tgctggagac
      901 ctgcctctac gccgccgacc tcgacgcggc cgagcgcttc taccgcgacg tgctcgggtt
      961 agccgtcgag tcgaaggtgg ctgggcgcca cgtcttcttc cgctgcggcg cggccgtctt
     1021 cctggtcttc aatccggagg ctacgcgccg cggcggaggg ccggtacccg cacacggcgc
     1081 gagcggccct ggccacgtcg ccttcgccgc ccggccggag gagctgcccg gctggcgcga
     1141 gcagctcgcg cgcggcggcg tgccgctcga gcaggaggtg cgctggccgg ggggcggcgt
     1201 ctcgctctac ttccgcgacc cggccggcaa cagcatcgag ctcgccacgc ccggtatctg
     1261 gggacaggag tagccgcggc gtatctgcca tcgcgagcat gagggcttga cgaggcttcc
     1321 attctattag aagatgaggc gaagccgttt cacgctggca caaccagatg accggttctt
     1381 gacgtaggtc aagcgagacc cacaaccgat ccagcccctg cgaggtggtg cgggagctag
     1441 agtggttgcg gcgtttgccg gtttccggcg tgcgaccgat cgtagtggag gaggagttat
     1501 gtcccaggat ccacgcttcg acgacctgat gcgggccgcg cgtgagggcc gcctgaaccg
     1561 ccgcgcgctc ctgaaacgcg ccgccgtcct tggactcagc gctccggcca tcgcggccct
     1621 gctagctgcc tgcggcggca gcagttcgac cagcaccagc gccgccggca ccagcgcgcc
     1681 gaccacctcc ggcggcagcg cggccaccgc cgccccgacc accgccgccg cggcgaccaa
     1741 ggcgccgacc accgccgccg cctcgccggt gaccaatcct ggagcagcga ccgcggagac
     1801 cgcctccggc ccggctggcg gcaagggcga cctgaagctc ctctggtggc aggcgcccac
     1861 catcatcaac ccccacatcg cgcaggggac caaggactac gacgcctcgc gcatcgtcta
     1921 cgagccgctg gccgacttcg acgccaacag caagatggtg ccgatgctgg cggccgagat
     1981 ccccagcctg gacaacggca tggtggccaa ggacggcctg agcgtcacct ggaagctgcg
     2041 ccagggggtc aagtggcacg acggccagcc gtttacggcc aacgacgtca agttcacctt
     2101 cgatctggtg agtgacccca aggccgccgc cacgacctcg ggcgtctacc tcaacgtcaa
     2161 gtcggtggac gtagtcgacg acaataccgc caaggtgaac ttcaagcagc cggagccggc
     2221 ctggtactac tccttccccg gcgccaacgg catgatcctg ccgcagcacg tcttcaagga
     2281 ttacaccggc gcgaacctgc gcaacgcccc ggccaacctc aagccgatcg gcaccggcgc
     2341 cttcaagctg accgacttca agccgggcga cgtcggcacc tacgagatca acaaggatta
     2401 ctgggacaag ggcaagccgc acttcgacag catcactatg aagggcggcg gcgatgcgcc
     2461 cagcgcggcc cgcgcggcgt tgcagaccgg cgagtccgac tggtcctgga atatccaggt
     2521 ctcaccgacg gtgctcaagt cgatggagac cgccggcaag ggcaaggtcg tgaactggcc
     2581 gggctcgggc accgagcgct tggagatcaa ctggtcggac ccgcagaccg aggtcaacgg
     2641 cgagaagtcg tactacaaga atccccaccc gcacttcaag gtacccgagg tacgccaggc
     2701 gctgaacaac tcgatcgacc gcaagacgat cgccgagcag ctctacggct ccggcggcgc
     2761 gccgaccggc cagacgcaga acgagaatac gccctatatg ctgcccgcct ccgacccggc
     2821 ggcgcagtgg aagttcgacc tgaaggcggc cgccgacctg ctcgacaagg ccggcgccca
     2881 gaagggcggc gacggcatcc gcacgctgaa cgggcgcaag atgtcctggg tctaccagac
     2941 ctcggtcaat gaggtgcgcc agaagcacca ggagatcgtc aagcaggcgc tgggctcgat
     3001 cggcgtcgag gtgcagatca agtcgatcga cgcctcggtc tacttctcgg gtgacgccgg
     3061 caacccggat accaccgggc acttctatgc cgacctcgag atgcatacca acggctgcgg
     3121 catcttcccg ctcaactggt acaagcgcta tctctcggcc aacccggaca ccgatatccc
     3181 gcagaagtcg aactcctggg cgccgtcgaa catcttccgc taccagagcg acgagttcaa
     3241 caccatgtgg gcgcaggcgc agaccgagct ggacccgcag aagtcgatcg cgctcttcca
     3301 gaacatgcag aagcttgtgc tgaaggacgt ggtcgagatc ggccagatcg cgcgcgccaa
     3361 cgtggcctgc gtcagcaacc ggctgaaggg ctacaacccg acccagtggg cctccgacgt
     3421 ctgggacatc aagaactgga cgacggccta ggtttcggcg cgccgctacg atctgcctca
     3481 gggggctgcc gctggcagcc cccatcttcg tgcccgccgc atcgcccacg gacaggagcc
     3541 ataccggcaa gcgctgccgc cctcaaggaa catgggcggc agcgagagcg gacccttcgg
     3601 ctgcgctcag aatgacaggg agagcaagag gcggacgaag acgatcggcg gcgtcgtacg
     3661 ccccaatccc ccattcatgt tatcctgagc gcagtcgaaa gatccgtact cgaccgtcag
     3721 catcccctgc agacggcctc ccccgacccc gaccacccca ccggccagcg tgagtttgcg
     3781 cctcccttca cgaggaatgg agacgctggc ggctattttg ctaccgtatt ccgcaatgcg
     3841 agtctgggtg aaatggctcg cttgacgaaa gccagcgcgt tatagcagta ttgcgttatg
     3901 gcggtggccg gatgtcggcc gcctcggtgg cgggccagcg gaggaggagc accagccgga
     3961 gcgacgccta gagcaaaccc tgacgagtcc cgactgcctg acctcgctag aggagatgag
     4021 gagacgatga atcaggatcc acgctacgat gaactgctgc gtgcggtgcg cgcgggccgg
     4081 atgagccggc gcgggctgct caagcgcgcc gcggtgctgg ggctggcggc cccggccatc
     4141 tccgccctct tggcggcctg cggcggtggg agcaacagca gcacaaccac cggcggcacg
     4201 accaccagcg gcacgggcgg caccagcacc accggcggca ccggcgcggc cagcacgccc
     4261 gccgcgaagg ttcccgcctc gccggtcttc aaccccagtg cggccacccc gcaggcgtcc
     4321 ggcgccaccg gccagcgcgg cggcaagggc gagctgaagt tgctctactg gcaggcgccg
     4381 accatcatca acccacacct ggcccagggc accaaagact tccaggcctc gcgcgtctgc
     4441 ctggagcccc tggccgactt cgacgccaac accaagatgg tccccttcct ggcgactgag
     4501 atccccagcc tggacaacgg cagcgtcgcc aaggacggca agagcgtcac ctggaagctg
     4561 cgccagggcg tcacctggca cgacggccag cccttcaccg ccaacgacgt caagttcacc
     4621 ttcgactacg tctccgacca ggcgaccgcc gccaccacct acggcagcta cgtcaacgtc
     4681 gactcggtcg acgcggtcga cgaccacacc gtcaaggtca acttcaagca gccggagccg
     4741 gcctggtacc agccattcac cggcgagaac ggcatgatcc tgccgcagca catcttcaag
     4801 gactacaaag gcgccaccgc ccgccaggcg ccggccaacc tcaagccgat cggcaccggt
     4861 ccctatcagg tgaccgactt caagccgggc gatgtcgtcg tctacgccat caacaagaac
     4921 tactggcagg acggcgcgcc gcacttcgac gg
//
LOCUS       DASUBI010000202         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_571216, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000202 DASUBI010000000
VERSION     DASUBI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571216"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="VFU72_03945"
     CDS             <1..1848
                     /locus_tag="VFU72_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422670.1"
                     /translation="VSGFVRAPATLDASIVNQASAFMPDREVRQIMGSANDNFLLVRV
                     DDPERASQTASEIQSFLDKRGVSSGSYTVRDPSGFTGSRELGTLLLLLRVFSYVGAVL
                     SSFLVANTIAAVMLEETRQIGVIKALGGSRWDAMRTYLLFAGIIGLSGAALGWGCGLL
                     FGRVLSRYLAGLSGLVLPAFSFSPREVVLALVVGLGVALSSAAVPAWLAARQRVAPLL
                     ANRGVVADFRRGFVQQLTHWLGRLGALPAMGLRNLARRPTRAGITLALVAASVAAFLA
                     TQALSNSVNFTVDELYALYGADGWVYFNQPMQAGFAQTLETDPNVTGAELWTTAQASI
                     GSVRTDAWGMPVDPTIYTVRLTAGTWLRPANPVAAVLTSNLAATLQARVGDVLPLDIG
                     KRTGLVQVVGIVDDQSTYLGATSTGKIFLSNDDLQQIAGGAAQVMALKLRSSDPGAVD
                     ASLHEIEQRFSQYHPVTLAMYRDKASSRQAINILTLMLDVMVVIVGVVGLAGIANTLL
                     INLTERRREFGVLRALGASSRQVIRLVITEALGLAALGCALGLVIGYPLARYLVVLTG
                     AQLFQLDFRLGPLSVFGIIVVALVATAAVATGPGLIAARLRPIRVLRYE"
     gene            1841..>2764
                     /locus_tag="VFU72_03950"
     CDS             1841..>2764
                     /locus_tag="VFU72_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5422671.1"
                     /translation="MNRARLRRALVVIVLLILLAAIIAGFVLSRGGSERTAVVTRGSL
                     QVTIQTVGRLAAKNPVTVRNDVDGSVLLVAAQTGDTVKQGDVVVQLDPAPYQQAVDQA
                     KQAETDAESALSAVEQGLPANPSPEQTAQRLAAVQKVNEAKDAVSQAQDNLAGTLVLA
                     PADGTLITVSVSAGQRVPAGSQIAQEADLTQLQLNVDLDEVDFPNVQTGMGASIRFDA
                     FPGQEVKGTITRISPVAQTTGGTTTFPLTIDFTTPQGVTLRPGMNASVGIETAIRQNV
                     LLIPQQALRTVGERSFVTVVTDGHHEEREIHV"
BASE COUNT          399 a          982 c          924 g          459 t
ORIGIN      
        1 gtgagcggct tcgtacgcgc gccggccacg ctcgacgcct cgatcgtcaa ccaggccagc
       61 gcgttcatgc ccgaccgtga ggtgcggcag atcatgggca gcgccaacga taacttcctg
      121 ctggtgcgcg tcgacgatcc cgagcgcgcc agccagaccg cgagcgagat ccaaagcttc
      181 ctggacaagc gcggtgtctc ctccggcagc tacacggttc gcgaccccag cgggtttacc
      241 ggcagccgcg aactgggtac cttgctcctc ctgctgcgcg tcttctccta cgtcggcgcc
      301 gtgctcagca gtttcctggt agccaacacc atcgcggcgg tgatgttgga ggagacgcgc
      361 cagatcggcg tgatcaaggc gctcggcggc tcgcgctggg acgcgatgcg cacgtatctc
      421 ctcttcgccg gcatcatcgg cttgagcggc gccgcgctcg gctggggctg cgggctcctc
      481 ttcggacggg tgctgtcgcg ctacctggcc ggcctctcgg gactcgtgct ccccgcgttc
      541 agcttctcac ctcgtgaggt cgtgctggcg ctggtggtcg gcctgggagt ggcgctcagc
      601 tccgcggccg tccccgcctg gctggccgcc cgccagcgcg tcgcgcctct gcttgccaat
      661 cgcggcgtcg tcgccgattt ccgccgtggg ttcgtccagc agctcacgca ctggctcggc
      721 cgactgggag cgctcccggc catggggctg cgcaacctgg cgcgccgtcc gacgcgcgcg
      781 gggatcacgc tggccctggt ggcggcctcg gtcgccgctt tcctggccac gcaagcgctt
      841 tcgaactcgg tcaactttac cgtcgacgag ctctacgccc tctacggcgc cgacggttgg
      901 gtctacttca accagccgat gcaggccggc ttcgcccaga cgctggagac cgacccgaac
      961 gtgaccggcg ccgagctctg gaccactgcc caggcttcga tcggcagcgt gcgcaccgat
     1021 gcctggggca tgcccgtgga ccccacgatc tacacggttc gcctgacggc gggcacctgg
     1081 ctgcgcccgg ccaacccggt cgccgccgtc ctcaccagta acctggcggc gacgctgcag
     1141 gcgcgcgtcg gcgacgtgct gccgctcgac atcggcaagc gcaccggttt ggtacaggtg
     1201 gtcggcatcg tcgacgacca gagcacctat ctgggcgcca cctcgaccgg caagatcttc
     1261 ttgagcaacg acgacctgca acagatcgcg ggcggtgccg cccaggtgat ggcgctcaag
     1321 ctgcgctcgt ctgatcccgg cgcggtcgac gccagcctgc atgagatcga gcagcgcttc
     1381 agccagtatc acccggtgac actggcgatg taccgtgaca aggcgagctc acgtcaggcg
     1441 atcaatatcc tgacgctgat gctcgacgtg atggtggtga tcgtcggggt ggtcgggctg
     1501 gccggcatcg ccaacacgct gctgatcaat ctcaccgagc gccgccgcga gttcggcgtg
     1561 ctgcgcgcgc tgggcgcctc cagccgtcag gtgatccggc tggtgattac cgaggcgctg
     1621 gggctggccg cactcggctg cgcgctcggg ctcgtgatcg gctacccgct ggcgcgctac
     1681 ctggtggtac tcaccggcgc gcagctcttc cagctcgact tccggctcgg accgctctcg
     1741 gtgttcggca tcatcgtcgt cgccctggtc gcgacggcgg cggtggcgac tggcccgggc
     1801 ctgatcgccg cgcgtctgcg gccgatccgg gtgctgcgct atgaataggg ccaggctgcg
     1861 ccgcgcgctg gtggtgatcg tcctcctgat cttgctggcc gcgatcatcg ccggcttcgt
     1921 gctctcgcgc ggcggctcgg agcggaccgc ggtggtcact cgtggctcgc tccaggtcac
     1981 cattcagacc gtcggacgtc tggcggccaa gaacccggta acggtgcgca acgatgtgga
     2041 tggcagtgtt ctgctcgtcg cggcgcagac cggcgacacc gtcaagcagg gcgacgtggt
     2101 cgtgcagctc gatccggcgc cgtatcagca ggcagtcgac caggccaaac aggccgagac
     2161 tgacgccgag tctgccctca gcgcggtcga gcaggggctg cccgccaacc cgagcccgga
     2221 gcagaccgcg cagcgcctgg ccgccgtaca gaaggtgaac gaggcgaagg acgccgtctc
     2281 gcaggcgcag gacaacctgg ccggcacgct ggtgctggcg cctgccgatg gcacgctgat
     2341 caccgtgagc gtcagcgccg gccagcgcgt gccggccggc tcgcagatcg cccaggaggc
     2401 cgatctcacg cagctccagc tcaatgtcga tctcgacgag gtcgacttcc ccaacgtcca
     2461 gaccggcatg ggcgcctcga tccgtttcga cgcctttccc ggtcaggagg tgaaaggcac
     2521 catcacgcgc atctcgccgg tggcgcagac gaccggcggc accaccacct tccccctgac
     2581 catcgacttc accacgcccc agggcgtcac gctgcggccg ggcatgaacg ccagcgtcgg
     2641 catcgagacc gctattcgcc agaacgtcct gctgataccc cagcaggcat tgcgcacggt
     2701 cggcgagcgc agcttcgtca ccgtggtgac ggacgggcat cacgaggagc gcgagatcca
     2761 cgtg
//
LOCUS       DASUBI010000203         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_785444, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000203 DASUBI010000000
VERSION     DASUBI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785444"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFU72_03955"
     CDS             <1..663
                     /locus_tag="VFU72_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5422672.1"
                     /translation="EGEYAGTGGRVHTGTPHEVALQTSVFSRFNVERVVRYAFELARG
                     RRKHLTSITKSNAMQYAMPFWDEVVVEVAADYPDVAVTSLLVDAAAALMVRAPERFDV
                     AVGSNLFADILTDLGGAIMGSLGLPPSANLAYSDEFPSLFEPVHGSAPDIAGQGLADP
                     LGAIWAGAMMLDYLGLGAAGKRVMRALEATTAARDVLPVDLGGHASTDQVAGAVIAHL
                     KD"
     gene            complement(842..1687)
                     /locus_tag="VFU72_03960"
     CDS             complement(842..1687)
                     /locus_tag="VFU72_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5422673.1"
                     /translation="MNIYMSVDMEGITGIVHRDMLSQEARDYERGRRLMTADAVAAVE
                     GLAQGGATYVLVNDGHGPMRNLLFEEFRSPAVLLSGTADSKAHCQVEAADLRHYDAAV
                     FVGYHAMARTPKAIAPHTIAGVAVAELRINGRPHGETGLNAAVLGSLGIPVVMVTGDT
                     TLEQEAKAFLGDAIQTVAVKEPVGTAAAICRPQQETLPEIRDAARRALESRGQVKPYN
                     PSEFRLEVDFFNVPQCDRASRVAGVERLGPLTMLVAGGSPWEQYAVLWSALRAALNAP
                     QSWLA"
     gene            1777..2169
                     /locus_tag="VFU72_03965"
     CDS             1777..2169
                     /locus_tag="VFU72_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422674.1"
                     /translation="MPHDEVPRSFQQHDYDETEGHTYGLAPVPVQIRHGDHVDVLQGG
                     HLHSLREDGSLEEHSIPASATNPVDCTRGHFEPGIGLGHEAGHQHGADCGHPLVPHAG
                     HMDYLVNGRLHFPHGDHCDDHGPVEVVH"
     gene            complement(2299..3000)
                     /locus_tag="VFU72_03970"
     CDS             complement(2299..3000)
                     /locus_tag="VFU72_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422675.1"
                     /translation="MHTAENRQQPRSGALSRRHLIRRGGLAGAAALGLALVQPAAAMQ
                     PATHTSAAPRDPNALTLHDTMRKLWEDHITWTRLFIVSFAAGLPDLDPTTQRLLRNQV
                     DIGDAFKPAFGAAAGTQLTQLLTEHILGAARLLAAAKAGDSAGVATAQAAWYDNANQI
                     AAFWNGLNPSAWPLDDMQMMMKDHLDLTLAEGVARLNGDFAADIAAYDQVHLEILAMA
                     DMLTAGFPLGGRARP"
     gene            3473..>3861
                     /locus_tag="VFU72_03975"
     CDS             3473..>3861
                     /locus_tag="VFU72_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5422676.1"
                     /translation="MMDRPWVDFDELRGKTDLVILPSGAVEVYGEHLPVGTDSIVVTA
                     IAKRVGEQLGAPVLPTLPVGFSRSLDDFPGTLNISTSTFKSYLRDTAESVLRWGFTRV
                     LFINSHRGNIGPIGEVAMELQRNQGVR"
BASE COUNT          573 a         1308 c         1379 g          601 t
ORIGIN      
        1 gagggcgagt acgccggcac gggcgggcgc gtgcacaccg gcacgccgca cgaggtggcg
       61 ctccagacct cggtcttctc gcgcttcaac gtggagcggg tggtgcgtta cgccttcgag
      121 ctggcgcgcg ggcggcgcaa gcacctgacc agcatcacca agagcaacgc catgcagtac
      181 gccatgccct tctgggacga ggtggtggtc gaggtggcgg ctgactaccc cgatgtggcc
      241 gtcacctcgc tgctggtcga cgcggcggcc gcgttgatgg tgcgcgcgcc cgagcgcttc
      301 gacgtggcgg tcggtagcaa cctcttcgcc gacatcctga ccgacctggg cggcgcgatc
      361 atgggcagcc tcggcctgcc gcccagcgcc aacctggcct acagcgacga gttcccctcg
      421 ctcttcgagc cggtgcacgg cagcgcgccg gacatcgccg gccaggggct ggccgacccg
      481 ctgggcgcga tctgggccgg tgccatgatg ctcgactacc tggggttagg cgcggccggc
      541 aagcgggtca tgcgcgcgct ggaggcgacg accgccgccc gcgacgtcct cccggtcgac
      601 ctcggcggcc acgccagcac cgaccaggtg gccggcgcgg tgattgctca cctgaaggac
      661 tgatggctaa aggacggcat cggggtaggg caagacggat ccttcgacaa gctcaggatg
      721 acagggtaag ggagaggcga tcgactacgt atatcgttgt cgtcagcccg ctattaaccc
      781 tgtcatcctg agcttgtcga aggatccgtc ctcacgcggc acaaatcgta ttcggcgggc
      841 gctacgccag ccaggactgc ggcgcgttga gcgcggcgcg cagggccgac cacaggacgg
      901 cgtactgctc ccaggggctg ccgccggcga ccagcatcgt cagcgggccg aggcgctcga
      961 cgccggccac gcgggaggcg cggtcgcact gcggcacgtt gaagaagtcg acctccagcc
     1021 ggaactcgga cgggttgtag ggcttgacct ggccgcgact ctccagcgcg cgtctggccg
     1081 cgtcgcggat ctcgggtagc gtctcttgct gcgggcggca gatcgcggcc gccgtgccga
     1141 ccggctcctt gaccgccacc gtctggatcg cgtcgcccag gaaggctttc gcctcctgct
     1201 ccagcgtggt gtcgccggtc accatcacta ccgggatgcc gagcgagccg agcaccgccg
     1261 cgttgaggcc ggtctcgccg tgcgggcggc cgttgatgcg cagctcggcg acggccaccc
     1321 cggcgatggt gtgcggcgcg atcgccttgg gcgtgcgggc catggcgtgg tagccgacga
     1381 agacggcggc gtcgtagtgc cgcagatcgg cggcctcgac ctggcagtgg gccttggagt
     1441 cggcggtgcc gctcaagagg accgccggcg agcggaactc ctcgaagagg agattgcgca
     1501 tcggcccgtg gccgtcgttg accagcacgt aggtcgcgcc gccctgggcc agcccttcga
     1561 ccgcggccac cgcgtcggcg gtcatcaggc gccggccgcg ctcatagtcg cgcgcctcct
     1621 gcgacagcat gtcgcggtgc acgatcccgg tgatgccttc catatcgaca ctcatataga
     1681 tgttcatgcc tgcaccctcc cttcgcgcgc atcatcgcac gggcgccttg ggcacgaaac
     1741 tggcgtggcg cgagcgagag acgagaggag cacgcgatgc cccatgacga ggtgccgaga
     1801 tcattccagc agcacgacta cgacgagacc gaggggcaca cctacgggct cgcgccggtg
     1861 ccggtccaga tccgccacgg cgaccacgtc gacgtgctgc agggcgggca cctgcactcc
     1921 ctgcgcgagg acggcagcct ggaggagcac agcatcccgg ccagcgcgac caaccccgtc
     1981 gactgtacgc ggggccactt cgagccgggc atcggcctgg gccacgaggc gggccaccag
     2041 cacggggcgg actgtggcca cccgctggtg ccgcacgccg gccatatgga ttacctggta
     2101 aacggccggc tgcacttccc gcacggagac cactgcgacg accacggccc ggtcgaggtc
     2161 gtccactgac gcgttcgtcg cagttggttt gatgggagct gggacagtga tcccactacc
     2221 gtgggccgcc gtggggttcg aattacccca cggcggctgt tgctctaccc gagcggaagc
     2281 tgagaattgg gaggacggtt acggcctcgc gcggccgccg agcgggaagc ccgcggttag
     2341 catgtcggcc atcgcgagaa tctccaggtg gacttgatcg taggcggcga tatcggcggc
     2401 gaagtcgccg ttgagccggg ccaccccttc ggccagcgtc aggtccaggt ggtccttcat
     2461 catcatctgc atgtcgtcga gcggccaggc gctcgggtta aggccattcc agaacgccgc
     2521 gatctggttg gcgttgtcgt accaggcggc ctgggccgtg gcgacccccg cggaatcgcc
     2581 cgccttggcc gcggccagga gccgggcggc gccgaggatg tgctcggtca ggagctgcgt
     2641 gagctgcgtg ccggccgcag cgccgaaggc cggcttgaag gcatcgccga tgtcgacctg
     2701 attccgcagc aggcgctggg tcgtcgggtc gaggtccggc agtccggcgg cgaagctgac
     2761 gatgaagagg cgcgtccagg tgatgtggtc ctcccacagc ttgcgcatcg tgtcgtgcag
     2821 ggtgagtgcg tttgggtcgc gcggcgcggc gctcgtgtgg gtggccggct gcatcgcggc
     2881 ggccggttgc accagcgcca ggccgagcgc ggccgcgccg gcgagccccc cacgcctgat
     2941 caggtgccgg cgactcaggg cgccggaccg aggttgctgc cgattctcag cggtgtgcat
     3001 ggtagatccc cctcgtctct cgatcatccg atgcgcggcg tgacacgagg tgccgttcag
     3061 gggccgcgcg cgggcggaag cagcgaccat gcgttgctga cgcggccggc gcagcccgcg
     3121 tgatgcccgc gcggccctcg gaggccggcg ctcgtctctg cgtggctggc agcggtagct
     3181 atcgtcgtcg ggctcgccat ggcgtacgat ccgcccgttc aataagtcca aaaacctctg
     3241 agttttctgt cttacgacca accgctggcg ctggattgct cgcctggggc gtgcaagttg
     3301 gcgcgctcgc cccgccgtgc tgtccagcgc cacggcacgc gcgagccggg tgtacaacca
     3361 cgcttgatcg ctcatcacgc gcgtgctagg gtgagcctgc tgattgccgg aggccgacgc
     3421 gccgggacgg atggcgctcg cccgtgaggg gaaggggacg accaccttgt acatgatgga
     3481 caggccctgg gtcgatttcg atgagctgcg gggcaagacc gacctcgtca tcctgcccag
     3541 cggcgcggtg gaggtctacg gcgagcacct cccggtcggc accgacagca tcgtggtgac
     3601 ggccatcgcc aagcgcgtcg gcgagcagct cggcgcgccc gtcctgccga cgctgccggt
     3661 gggcttctcg cgctcgctcg acgacttccc cggcacgctg aacatctcga ccagtacgtt
     3721 caagtcgtac ctgcgcgaca ccgccgagag cgtgctgcgc tggggcttca cgcgcgtgct
     3781 cttcatcaac agccaccgcg gcaacatcgg gcccatcggc gaggtcgcca tggagctgca
     3841 gcgcaaccag ggcgtgcgct g
//
LOCUS       DASUBI010000204         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1275650, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000204 DASUBI010000000
VERSION     DASUBI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275650"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFU72_03980"
     CDS             <1..235
                     /locus_tag="VFU72_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422677.1"
                     /translation="NGASRRGRRGRPEPASLRRFPPCPRRGDRLVEIAAFLLVLFTAI
                     YGSTLLSERAFRALRLITNRGCRSMRQKNNGYL"
     gene            complement(414..>2079)
                     /locus_tag="VFU72_03985"
     CDS             complement(414..>2079)
                     /locus_tag="VFU72_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422678.1"
                     /translation="DAVGAFEDLLRATLRPRLRRDFPAWILKAPFVEANRGVFMLRFA
                     PKWQQWAATEAQRIGIDPRNTLAGEAGWNQLTMLYDALSVQLRRIADRRSAFSWLVIT
                     SRINPTDLLPAACASTSEAVLFFALSEVAANPRPSRSTELIASRWWLAPQALTDLDEL
                     CSVGMLLFNVYNARRRVAKGQSLRLGGPHLVPEITDIDDTAARSIFLYDSRLRLAGGR
                     GNDLGSLPNLVTDRDSANIVTWADLRGQDLVSRERLAQLLTQYGPVFPLGTKIQDLAP
                     AAFCGGIGLTLETQAAATALDAAWNYLAQRGATWKSNAGEWSQYGYLRCPRNWFIRHL
                     EAGPALPELLGSSWRAGATNRGVITLLTRKQVLLPVGRYFVVNLTSATSLLMHSFERA
                     REGAAANEWGSSFEAAVQKAIDNTSWKPTGALRSVIGKRVKDENGNVITDIDAVAFHT
                     ETLWLISAKSFQTGPGYRIGAYDAVVRIARKAVDASEQWNGCLERIRKRPSALGVDLP
                     AGCTLAGLVVTPFVPYVPIDSSAAQSVGDLCHVSSIHELIAAASRA"
BASE COUNT          326 a          670 c          726 g          357 t
ORIGIN      
        1 caatggcgcg tcccgccgcg gtcggcgtgg gcgccctgag cccgccagcc ttcggcgctt
       61 tccaccctgc cctcggcgcg gcgatcgtct cgtcgagatc gccgccttcc tcctcgtcct
      121 gttcaccgcg atctacggat cgacgctcct aagcgagcgc gccttccgcg cgctgcgcct
      181 catcaccaac aggggttgcc gtagcatgcg ccaaaaaaac aacggatatc tctgaccagc
      241 gcaaacacgc cccgatagtg gggatgaggc ctccgggaac gttagggatc cgtgatctgg
      301 ctgggtcggt cgagccggct ggtgacgcgg acatctctcg gccgcgtact ggcgaagtcg
      361 cgaatcaagt gtcccggtgg gctaagcgca gtgctcactg aggttaggca gttctacgcc
      421 cgcgacgccg cggcgatgag ctcatggatg ctcgagacgt ggcagaggtc tccaaccgac
      481 tgcgcggccg aggagtcgat cggcacgtac gggacgaacg gcgtgacgac cagcccggcg
      541 agcgtgcatc ccgcaggcag gtccacccca agcgcggacg gacgcttccg gattctttcg
      601 aggcagccgt tccactgctc gctcgcatcc acggctttgc gcgcgatcct taccacggcg
      661 tcgtaggcgc cgatgcggta gccgggtccg gtctggaatg acttcgcgct gatgagccac
      721 agggtctcgg tgtggaaggc gacggcgtcg atgtcggtga tgacgttgcc gttctcgtcc
      781 ttgaccctct tgccgatcac cgagcgcagc gcgccggtcg gcttccagga cgtgttgtcg
      841 atcgccttct gcacggcggc ttcgaacgaa ctgccccact cgttggccgc ggccccttcc
      901 ctggcccgtt cgaagctgtg catcagcagc gaggtggcgc tcgtgagatt gacgacgaag
      961 tagcgcccga ccggcagcag cacctgcttg cgggtcagca gcgtgatcac gccgcggttc
     1021 gttgcgcctg cacgccagga tgagccgagc agttcgggca gcgccgggcc ggcttccagg
     1081 tggcggatga accagttgcg ggggcagcgc agatagccgt attgggacca ttctcccgcg
     1141 ttagacttcc atgttgctcc gcgctgcgcg agatagttcc aggcggcgtc cagggccgtg
     1201 gcagcggcct gagtctccag ggtcaggccg atgccgccgc agaaagccgc gggtgcgagg
     1261 tcctggattt tcgtgccgag cgggaagacc gggccgtact gcgtgagcag ttgcgcgaga
     1321 cgttcgcggg agacgaggtc ttggccgcga aggtccgccc aggtgacgat gttcgcggag
     1381 tcgcggtccg tgaccaggtt cgggagggag cccaggtcgt tgccgcggcc gcctgcgagt
     1441 ctcagcctgc tgtcgtagag gaagatggag cgcgcggcgg tgtcgtcgat gtctgtgatc
     1501 tcggggacca ggtgcggtcc accgagcctg aggctctggc ccttggccac ccgtcgacgg
     1561 gcgttgtaga cgttgaacag gagcatcccg accgagcaca gttcgtccag gtcggtcagc
     1621 gcctgcggag cgagccacca acggctcgcg atcagctcgg tcgacctgct cgggcgcggg
     1681 ttggccgcca cctcgcttag ggcgaagaag agcacggcct cggaggtcga ggcgcaggcg
     1741 gcgggcagca gatcggtggg attgatccgg ctagtgatca ccagccagct gaaggccgat
     1801 cgtcgatcag cgatccggcg tagctgaacg ctcagcgcgt cgtagagcat ggtcaactgg
     1861 ttccatccgg cctcgcccgc aagggtgttg cgggggtcga tgccgatccg ctgtgcctcg
     1921 gtggcggccc actgctgcca cttcggcgcg aaccgcagca tgaacacccc ccggttcgcc
     1981 tcgacgaacg gcgccttgag gatccaggcg gggaagtctc ggcgcaggcg agggcgcagg
     2041 gtcgcgcgca gtaggtcttc gaacgcgccc accgcatcc
//
LOCUS       DASUBI010000205         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_21774, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000205 DASUBI010000000
VERSION     DASUBI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21774"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VFU72_03990"
     CDS             complement(<1..1642)
                     /locus_tag="VFU72_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422679.1"
                     /translation="MADYYDLGAHSWPITTISPEAQRWFDRGLNWTYGFNHVEARRCF
                     ERAIEHDPNCAMAHWGVAYAAGPNYNRTWAMFEPAELRKTVARTFAASQRALAVCEHA
                     APGEQALISALAARYPQAEPVEDCAIWNDDYADAMRAVYRDYRDDLDVAALFAEALMN
                     RTPWQLWDLPTGQPAEGASTLEARAVLERALEQDAARKHPGILHMYIHLMEMSPEPEV
                     ALRAADWLRELVPDAGHLRHMPTHIDVLCGNYHDVVVSNSAAIVADDKYLAREGAMNF
                     YTLYRVHDFHFKVYGAMFLGQCETALRTADEVLGTIPRELLERTEPNMADWLEGFLGI
                     RLHVLIRFGQWQAILDTPLPEDQELYCVTTALSHYARGVALAASGRVAEADAEQARFE
                     AAAARVPESRMLFNNRCLDILEIARAMLAGELEYRRDNYEEAFAQLRHAVALDDGLLY
                     DEPWGWMQPARHALGALLLEQGRVEEAAAVYRADLGLDGSLARACQHPENIWSLHGYH
                     ECLERLGEHEQAALVKQRLQLAAARADIPIHASCYCRMA"
     gene            complement(2036..>2211)
                     /locus_tag="VFU72_03995"
     CDS             complement(2036..>2211)
                     /locus_tag="VFU72_03995"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5422680.1"
                     /translation="PCGWLRDRYGVSWQVVPSRLIELLGDQDRERANRVMQAMLRMSK
                     IEIAPLERAYAGE"
BASE COUNT          328 a          754 c          779 g          350 t
ORIGIN      
        1 ccgccatgcg gcagtagcag gaggcgtgga tcgggatgtc agcgcgggcc gccgccagct
       61 ggagccgctg cttgaccagc gcggcttgct catgctcccc gagccgctcc aggcactcgt
      121 ggtagccgtg caggctccag atgttctcgg ggtgctggca ggcccgcgcc aggctgccgt
      181 cgagccccag gtcggcgcgg tagaccgccg cggcctcctc gacgcgcccc tgctccagaa
      241 ggagcgcacc cagcgcgtgc cgggccggct gcatccagcc ccagggctcg tcgtagagga
      301 gcccgtcgtc gagcgccacg gcgtggcgca gctgggcgaa ggcctcctcg tagttgtccc
      361 ggcggtactc cagctcgccg gccagcatag cgcgcgcgat ctccaggatg tccaggcagc
      421 ggttgttgaa gagcatgcgc gactcgggca cgcgggcggc ggcggcctcg aagcgtgcct
      481 gctccgcatc cgcctcggcg acgcggccgc tggcggccag ggccacgccc cgtgcgtagt
      541 ggctgagcgc ggtggtgacg cagtagagct cctgatcctc gggcagcggc gtgtcgagga
      601 tggcctgcca ctggccgaag cggatcagca cgtgcagccg gatgccgaga aaaccctcca
      661 gccagtcggc catgttcggc tcggtcctct ccagcaactc ccggggaatg gtgccgagca
      721 cctcatcggc ggtccgcagc gccgtctcgc actggcccag gaacatcgcg ccatagacct
      781 tgaagtggaa gtcgtgcacc cggtagagcg tgtagaagtt catggcgccc tcgcgcgcca
      841 ggtatttgtc gtcggccacg atggcggcgc tgttggagac gaccacatcg tggtagttgc
      901 cgcagaggac gtcgatgtgg gtcggcatgt ggcgcaggtg gccggcatcg ggcacgagct
      961 cacgcagcca gtcggccgcc cgcagggcga cttccggctc gggcgacatc tccatcaggt
     1021 ggatgtacat gtggaggatg cccgggtgct tgcgcgcggc atcctgctct agcgcccgct
     1081 ccagcaccgc gcgcgcctcc agggtgctgg cgccctcggc cggctggccg gtcggcagat
     1141 cccagagctg ccagggggtg cggttcatga gcgcctcggc gaagagcgcg gccacgtcca
     1201 ggtcatcacg atagtcgcgg tagacggcgc gcatggcgtc ggcgtagtcg tcgttccaga
     1261 tcgcgcagtc ctcgaccggc tcggcctgcg ggtagcgcgc ggccagcgcg ctgatcagcg
     1321 cctgctcacc gggtgcggca tgctcgcaga cggccagggc acgctggctg gcggcgaagg
     1381 tacgcgcgac ggtcttgcgg agctcggcgg gctcgaacat cgcccaggtc cggttgtagt
     1441 tcggcccggc ggcgtaggcc acgccccagt gggccatggc gcagttcggg tcgtgctcga
     1501 tggcgcgctc gaagcagcgc cgcgcctcca cgtgattgaa gccataggtc cagttgaggc
     1561 cgcgatcgaa ccagcgctgc gcctcgggcg agatggtcgt gatcggccag ctgtgagcgc
     1621 ccaggtcgta gtagtcggcc atgagactct ccctctccct ccgctcggac gccccagcgc
     1681 ggcgtgatgc tgttcgttgt tggtctggcc ttcatcctag caggaaagcg accggcgacc
     1741 aagcacctgc gaagcagcct cacgccgcaa tcactcgcgg cccgatgatg gtgtcaggga
     1801 gagggcggcg gtgccggcgc acgctcacgc gggcgtagcg ggctatcctc catgttgaag
     1861 agagccccct tgctgcggct caggatggca ggggtagcgg ctatccgctc cctgtcgtcc
     1921 tcagggccgc ggccgaatga gctctccgac gcttctatac aaaccgcgcg ccaggggact
     1981 gatgctcagg tcatgcaaca agagactccg aggcctccgc gctggcgaac acgcgctatt
     2041 cccccgcgta ggcgcgctcg agcggtgcga tctcaatctt ggacatccgc agcatggcct
     2101 gcatgacgcg gttggcccgc tcgcggtcct ggtcgcccag cagctcgatg agtctcgacg
     2161 gcaccacctg ccaggagacg ccatacctgt ccctcagcca gccgcaggga c
//
LOCUS       DASUBI010000206         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_21719, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000206 DASUBI010000000
VERSION     DASUBI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21719"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..380
                     /locus_tag="VFU72_04000"
                     /pseudo
     CDS             138..380
                     /locus_tag="VFU72_04000"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
     gene            406..2421
                     /locus_tag="VFU72_04005"
     CDS             406..2421
                     /locus_tag="VFU72_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5422681.1"
                     /translation="MPTPQITPRQLQRCAYLYIRQSTMQQVVTNIESTARQYALRERA
                     LALGWPAERIVVIDQDLGQSGASATDRAGFQRLVAEVGLAHVGLVLGLEVSRLARCSS
                     DWHHLLELCALTGTLILDEDGLYDPATFNDRLLLGLKGTMSEAELHLIHARLQGGIRN
                     KARRAALKLHLPIGFCYTERDEIVLDPDRQVQDTIRFVFRTFQRTGSASATVRAVRRA
                     GVLFPRRLRSGPHQGELVWSELRHDEVLRLLHHPGYAGAYVFGRTRTWKTADGKVHIT
                     ALPRDEWQVTIRDAHAGYLTWVEYEANLAQLAANSQAYSPPRLRPPREGPALLQGLVL
                     CGRCGARMSVRYHQRGGRRIVPDYVCQREGVARGTPPCQRFLGRDVDAAVGTLLLAEM
                     TPAAVAATLAVHDEVRARTADAARLRQPAVERAQYEANLAQRRYLRTDPDNRLVAGVL
                     EAEWNDKLHALAAARETAERQRQTDEAHLSAVERAALLALPDEFARLWRDPQTTDQER
                     KRLVRLILEDVTLLKTAQIVVQVRFKGGATRTLAVPLPPPFAAARLTPGATLAAIDRL
                     LDQTTDAGIAAALNAQGYRTFAGLPFAATHVGQLRRTHRLKDRYTRLREAGLLTAEEL
                     AARLGVRPQTIWRWYRGGRLRGERYNDRGTCLFWPPADELGRDNEDA"
BASE COUNT          381 a          940 c          851 g          369 t
ORIGIN      
        1 gaaccccgca cggccataca tgctccgctt gaccaacttc aaccgattta cttgtccctc
       61 cacttgcccg gacggacgca ccgacgacta cacccgatgg cttcgatgag gccgtcccgt
      121 ttaccgttac ccaccctttc caccccctcg tcggccagcg gttcaccgcc gtgatgcagc
      181 gcaccgccta cggcgagccg cgcgtgtttt accaggaccc tgccaccgcg caggtgcggt
      241 ccttcccact cgcgtggacc gatctggccc cacccgacgc cttccggctg gtcgccgccg
      301 accgcgctat cctgcgcctc accgatctgc gcgccttggc ggcactagta cacgggctca
      361 gcgcggcgcg caggcgctga ccacgcctag ggaggaaggc aggccatgcc gacaccgcag
      421 atcacgccac ggcagttgca gcgctgcgcc tatttgtaca tccggcagag cacgatgcag
      481 caggtggtga ccaacattga gagcaccgcg cggcagtacg ccctgcgtga gcgtgcgctg
      541 gccttgggtt ggccggccga gcgcatcgtc gtcatcgacc aggacctggg gcagtcgggc
      601 gcctcggcca ccgaccgggc tggcttccag cggctggtcg ccgaggtcgg cctcgcgcat
      661 gtcggcttgg tgctcgggct ggaggtctcg cgcctggccc gctgctccag cgactggcat
      721 cacctgctcg aactctgcgc cctgaccggc accctcatcc tcgatgagga tggcctctac
      781 gatcccgcca ccttcaacga ccggctgttg ctcgggctga agggcacgat gagcgaggcc
      841 gagctgcatc tcatccacgc ccgcctgcag ggtggcatcc gcaacaaggc tcgccgcgcg
      901 gcgctcaagt tgcacctgcc gatcgggttc tgctacaccg aacgcgacga gattgtgctc
      961 gaccccgacc ggcaggtgca ggacaccatc cgcttcgtct tccgcacctt ccagcggact
     1021 ggctcggcca gcgccaccgt ccgggccgtc cgccgcgcgg gcgtgctttt tcctcgtcgg
     1081 ctgcgcagcg gtccacacca gggcgaactc gtctggagcg aactgcgcca cgacgaggtg
     1141 ctgcgcctgc tgcatcaccc cggctacgcc ggcgcgtatg tcttcgggcg gacgcgcacc
     1201 tggaagaccg ccgacggcaa agtccatatc accgcgctgc cgcgcgacga gtggcaggtc
     1261 acgatccgcg acgcgcatgc tggctacctc acctgggtcg agtacgaggc caacctggcg
     1321 cagctagcgg caaacagcca agcgtatagc ccgccgcgcc tgcgcccgcc gcgcgagggc
     1381 ccggcgctgc tccaggggct ggtgctctgc ggccgctgcg gcgcgcggat gtcggtgcgc
     1441 taccaccagc gcggcgggcg gcgcatcgtg cccgactacg tttgccagcg cgagggcgtg
     1501 gcccgcggta ccccgccgtg ccagcggttc ctggggcgcg atgtcgacgc cgccgtcggt
     1561 acgctcctgc tggcggagat gacgccagcg gcggtcgccg ccaccctcgc cgtgcacgac
     1621 gaggtgcggg cgcggactgc cgacgcggcc cggttgcgcc aacccgccgt ggagcgggcc
     1681 cagtatgagg cgaatctggc gcagcgccgc tacctgcgga ccgatcccga taaccgtctg
     1741 gtggccgggg tgctggaagc cgagtggaac gacaagctgc acgcgttggc ggcggcacga
     1801 gagacggcgg agcgccagcg ccagacggac gaggcccacc tcagcgcggt cgagcgggcc
     1861 gcgctcttgg cgttgccgga cgagttcgcc cgcctctggc gcgatccgca gaccaccgac
     1921 caggagcgca aacgcctggt gcggctcatc ctcgaggacg tcaccctact aaagaccgcc
     1981 cagatcgtcg tgcaggtgcg cttcaagggc ggcgcgaccc gaaccctcgc ggtgccgctc
     2041 ccgccgccgt tcgctgcggc gcgcctcacc ccgggggcca ccctcgccgc aatcgaccgc
     2101 ctgctcgacc agacgacgga cgcggggatc gcggcggcac tgaatgcaca gggctatcgc
     2161 acgttcgccg gactgccctt cgcggcgacg catgtcggcc agctccgccg cacacacaga
     2221 ctgaaagacc gctatacgcg actccgcgag gcggggctgc tgacggcgga ggaactggct
     2281 gcccggctcg gcgtccggcc gcagacgatc tggcgctggt atcggggcgg gcgcctgcgc
     2341 ggcgaacgct acaatgaccg tgggacgtgc ctgttctggc cgccggcgga cgagctgggc
     2401 cgcgacaacg aggacgcctg aggccgtgat agcggcgacg aggtgcagta tgcaacagga
     2461 tcggaactcc acgcgtggtc cagtgccgcc tgcaccgccg cctggtcccg gcggatcccc
     2521 gcggcgaagt cgcgcacctc g
//
LOCUS       DASUBI010000207         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_349162, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000207 DASUBI010000000
VERSION     DASUBI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349162"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VFU72_04010"
     CDS             <1..1376
                     /locus_tag="VFU72_04010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5422682.1"
                     /translation="ALGQVACGYPDVTSIAEIVQGVAARARELPAGSWVHGRGYDDNK
                     LAERRHPNRHDLDEAAPNHPVIIVNGSGHMCAVNSLALKLAGVTRDTPDPSGGHIVRD
                     EHGEPTGVLQETAQELVRRVIPPPTVEDQVAALKRCSDAYVAAGITSSTDASSTAAQQ
                     IEAYQQAVRAGALKLRTTMMIREHLLPHLLGLGIKQGFGDDHLRVGPVKLFIDGSLIG
                     RTAAVTRPFLEDPRPDNLGLTMMSQEELDNYVWQAHAAGFQIAIHAIGDRGIEMVLEA
                     YGKALERLPRADHRHRIEHCGILRPDLIERIAAMRLVIVTQPIFITEYGDGFIRHLGL
                     DRIQLTYPFRSLLDAGIPIVFSSDCPVSAFEPLKSIEVAVTERTGSGRSYALEEAISV
                     EEALPLYTVNGAYATFEEQRKGRLRPGMLADFAVLEQDPRQVEPEAIASVPISQTIIG
                     GQTVYES"
     gene            1398..2351
                     /locus_tag="VFU72_04015"
     CDS             1398..2351
                     /locus_tag="VFU72_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422683.1"
                     /translation="MQLPTVGMDLVDKSNARGALDASKRAAARGVPMVWSTTGPRAPD
                     PMSFFAAVAVETEQLGFGTAIVPTYPRHPTVIATQALALDQLAPGRFRLGLGPSHQPT
                     ISGALGLPMGKPLDHLREYVTITRDLLWNGGADIDGQYYRVHSQMNGTANVPIYISAL
                     RSRAFRLAGEVADGAISWMCPLGYLRDVAAPAMRQGAELAGRSTPRMVAHIPVVMTNE
                     RARMVELARPRVGGYARLPFYAAMFADAGYPVGSDAQVSEDLLDHLVVWGDPGEVRRR
                     LSNALGQGVDELLVTLLPGDEPAKDEEALSRVLTELSQESR"
     gene            2501..>3110
                     /locus_tag="VFU72_04020"
     CDS             2501..>3110
                     /locus_tag="VFU72_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5422684.1"
                     /translation="MTAAGEELRGKIALVTGAGSGIGRAIALRFAAEGATVALAGRTR
                     ETLEASARAIGAAALPVPLDVTDPESVAAGVLAVGQRLGPVDILVNNAGIGTTKSVVD
                     VEPEEWERVFAVNVRGVYLCTRAVLPGMLERGSGTIVNIASALGLVGVPGRAAYAASK
                     GAVIALTKQVAVEYAAQGIRCNCICPGTVDSPWVTRLLDQADA"
BASE COUNT          478 a         1123 c         1079 g          430 t
ORIGIN      
        1 ccgcgctggg tcaggtggcc tgcggctacc cagacgtgac ctcgatcgcc gagatcgtcc
       61 agggggtcgc ggcgcgggcg cgcgagctgc cggccgggag ctgggtccac ggccggggct
      121 acgacgacaa caagctggcc gagcgccgcc accccaaccg gcacgatctg gacgaggccg
      181 cgcccaacca cccggtgatc atcgtcaacg gctccggcca tatgtgcgcc gtcaactcgc
      241 tggcgctcaa gctggccggg gtgacgcggg acacgcccga cccctccggc ggtcacatcg
      301 tgcgcgacga gcacggcgag ccgaccggcg tgctgcagga gaccgcacag gagctcgtgc
      361 gccgggtgat cccgccgccg acggtggagg atcaggtcgc ggccctcaaa cgctgcagcg
      421 acgcctacgt ggccgctggg atcacctcca gcaccgacgc cagcagcacg gcggcccagc
      481 agatcgaggc ctaccagcag gccgtgcgcg cgggcgcgct caagctgcgg accacgatga
      541 tgatccgtga gcacctcctg ccccacctgc tcggcctggg catcaagcag ggcttcggcg
      601 acgatcacct gcgtgtcggc ccggtcaagc tgttcatcga cggcagcctg atcgggcgca
      661 ccgccgccgt gacgcgcccc ttcctggagg atccccgccc ggataacctc ggcctgacca
      721 tgatgtcgca ggaagagctg gacaactacg tctggcaggc gcacgcggcc ggcttccaga
      781 tcgcgatcca cgccatcggc gaccgcggca tcgagatggt gctggaggcc tacggcaagg
      841 cactggagcg gctgccgcgc gccgatcatc ggcaccgcat cgagcactgc ggcatcttgc
      901 ggcccgacct gatcgagcgc atcgccgcca tgcgactggt gatcgtcacc cagccgatct
      961 tcatcaccga gtacggcgac ggcttcatcc gccacctggg cctggaccgc atccagctca
     1021 cctacccctt ccgcagcctg ctcgacgccg gcattcctat cgtcttctct tccgattgcc
     1081 cggtctctgc cttcgagccg ctcaagagca tcgaggtcgc cgtgaccgag cgcaccggca
     1141 gcggccgctc ctacgccttg gaggaggcga tctcggtcga ggaggcgctg ccgctctaca
     1201 ccgtcaacgg cgcctacgcc accttcgagg agcagcgcaa gggacgcctc cggccgggga
     1261 tgctggccga cttcgccgtg ctggagcagg acccgcgtca ggtcgagccg gaagcgatcg
     1321 cgtcggttcc catcagccag acgatcatcg gcggacagac ggtctacgag tcgtagtcta
     1381 ggcaagcgag gcgagcgatg cagttgccaa ccgtcgggat ggacctcgtc gacaagagta
     1441 acgcccgcgg cgcgctagac gcatcgaagc gtgctgccgc gcgcggcgtg ccgatggtct
     1501 ggtcgaccac cggcccccgc gcgccggacc cgatgagctt cttcgccgcc gtcgcggtcg
     1561 agaccgagca gctcggcttc ggcaccgcga tcgtgccgac ctacccgcgc cacccgaccg
     1621 tgatcgcgac gcaggcgctg gcgcttgatc agctggcgcc cggccgtttc cggctcggac
     1681 tcgggcccag ccaccagccg acgatcagcg gcgcgctcgg gctgccgatg ggcaagccgc
     1741 tcgaccacct gcgcgagtac gtcaccatca cgcgcgacct cctctggaac ggcggcgccg
     1801 acatcgacgg ccagtactac cgggtgcact cgcagatgaa cggcaccgcg aacgtgccga
     1861 tctacatctc ggcgctgcgc tcgcgcgcct tccgcctggc cggcgaggtg gccgacggcg
     1921 cgatctcctg gatgtgcccg ctggggtacc tgcgcgacgt agcggccccc gcgatgcgcc
     1981 agggtgcgga gctggctgga cgatccacgc cacgcatggt ggcccacatc ccggtcgtga
     2041 tgaccaacga gcgcgcccgt atggtcgagc tggcgcggcc gcgcgtcggc ggctacgcgc
     2101 gcctcccctt ctacgccgcc atgttcgccg acgccggcta cccggtcggc tcggatgccc
     2161 aggtcagcga agatctgctc gatcacctgg tcgtctgggg cgatccaggc gaggtgcgcc
     2221 gccggctctc caacgcgctg ggccagggag ttgacgagct gctggtcaca ctcttgcccg
     2281 gcgacgagcc cgcaaaggac gaggaggcgc tgtcgcgggt tctgaccgag ctgagccaag
     2341 aatcccggta ggggcggctc accgtggccg cccggctgcc ccaacgggaa tggcacgggg
     2401 aagcatggaa ccgtgcgtgg caaacgacga tgcccggcaa gagggcggca acggcaggca
     2461 cgggggcccg cccctagcgg ttcgagagcg aggagcgtcc gtgacggcag caggcgagga
     2521 gctgcggggc aaaatcgcgc tggtgaccgg cgccggctcg ggcatcggcc gcgcgatcgc
     2581 gctgcgattc gccgccgagg gcgcgaccgt cgccctggcg ggccgcaccc gcgagacgct
     2641 ggaggcgagc gcgcgcgcca tcggcgcggc ggccctgccg gtgccgctcg acgtgaccga
     2701 tcccgagagc gtggcggccg gcgtgctcgc ggtcggccag cggcttggcc cggtcgatat
     2761 cctagtcaac aacgccggca tcgggacgac caagagcgtg gtcgatgtcg agccggaaga
     2821 gtgggagcgc gtcttcgcgg tcaacgtgcg cggcgtctac ctctgcactc gcgccgtctt
     2881 gcccggcatg ctggagcgcg gctccggcac catcgtcaat atcgcctcgg cactgggtct
     2941 ggtcggcgtg cccgggcgcg cggcctacgc cgccagcaag ggcgcggtca tcgccctgac
     3001 caagcaggtg gcggtcgagt acgccgcgca ggggatccgc tgcaactgca tctgccccgg
     3061 cacggtcgat agcccctggg tgacgcgcct gctggatcag gctgacgctc
//
LOCUS       DASUBI010000208         8335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_310724, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000208 DASUBI010000000
VERSION     DASUBI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310724"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFU72_04025"
     CDS             <1..435
                     /locus_tag="VFU72_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5422685.1"
                     /translation="EDAYDRHEPTHAALRQATVEWFPRLKNVAFTHAWGGPLGMPRDW
                     LPTMSYDRAAGIASSRGYTGQGVATANLAGRVLTDLILERETPLTELPLVNHQSPDWE
                     PEPLRWLGVRYVQRGYQRLDEQSERSGRAPNGRTLVERLGSH"
     gene            complement(495..1715)
                     /locus_tag="VFU72_04030"
     CDS             complement(495..1715)
                     /locus_tag="VFU72_04030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5422686.1"
                     /translation="MAEGEGEGEGARGPLDGIRVLDLSRVLAGPYCTMVLGDLGAEVI
                     KIEQPGSGDDTRAWGPPWAGGESAYYLSINRNKQSVTLNLKSERGREILRALARQSDI
                     VVENFKVGALDRMGIGYDALAAENPGLIWCSITGYGQTGPYADHPGYDFVAQGEAGIM
                     SITGEAEGEPTKVGVAIVDITTGLFAGNAILAALHARDLTGRGQRIDAALLTSAVAWL
                     ANVGSNYLISGKVPGRLGNAHPNIVPYQTFRARDRWMTVAVGNDRQFRALAGIVGRSE
                     LAEDARYATNPARVANRDTLVALLEEIFATRDADDWLAAMNAAGIPSGPINTVDRVFA
                     HPQVLHRDMLVEVPHPTAGSVRLAGIPFTLSGTPASVRLHPPLLGEQTEAVLHERLGL
                     SPDEIARLRDEGVI"
     gene            complement(2001..2297)
                     /locus_tag="VFU72_04035"
     CDS             complement(2001..2297)
                     /locus_tag="VFU72_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEU5422687.1"
                     /translation="MIVKEYTKTGRSCRVTFELPCEVDAESVSLLGDFNDWDPAARQL
                     KRRKDGSFATTISLKPGTDYRFRYLLDGDRWVNDTAADRHEPNRFGSEDSVISL"
     gene            complement(2487..2984)
                     /locus_tag="VFU72_04040"
     CDS             complement(2487..2984)
                     /locus_tag="VFU72_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422688.1"
                     /translation="MSQQERGVLRCRPLRPADRDFVLSLAARLEAAGMPPWREPQAMR
                     AFHRRYAEALLATEGPEQLALIAEDAAGRPLGVVHVTGAESGLSGEAQGYLATLAVAA
                     EAEGQGVGRALMAAAEEWARSRGYRLLALEVFAANERARAFYRRLGYQEETLKLVKEL
                     RPDEG"
     gene            complement(3106..3288)
                     /locus_tag="VFU72_04045"
     CDS             complement(3106..3288)
                     /locus_tag="VFU72_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422689.1"
                     /translation="MAAHQRAQMGMILANAHLVTDAVNGAACSKGSTAASDATKQVKQ
                     MLADYDAQHDDVPEFP"
     gene            complement(3403..3798)
                     /locus_tag="VFU72_04050"
     CDS             complement(3403..3798)
                     /locus_tag="VFU72_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422690.1"
                     /translation="MSTRLASADALTRSVIPTVLISSVVFSLALLPDPLQLLLPLVAF
                     GSGYYFRPRRTWVIWLASALIVSAGFAALWLLGYQPPKTADPGPANAAGIAFDTGAYF
                     VYLAALALAPLWFGRRFAERRTLEAPPAR"
     gene            complement(3827..4180)
                     /locus_tag="VFU72_04055"
     CDS             complement(3827..4180)
                     /locus_tag="VFU72_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422691.1"
                     /translation="MAGTHESTGEPLMSRLFSAPRTRAGLGALALLGLHVFFMLLWRL
                     GPRFGAGGGGETFFSNPWHAAFILLAGATAIAAGPLALFALIRRRERSILLYPVIVYA
                     LFVAAFALGEVLSPH"
     gene            4398..5534
                     /locus_tag="VFU72_04060"
     CDS             4398..5534
                     /locus_tag="VFU72_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5422692.1"
                     /translation="MTVELATLRAEFPIAEEWAYLNHAAIGPFSRRTEAAIAAVSGAF
                     ARPPSMGNAYGEAIETARGNVAKFVGGTPDGVAFVGSLADAMSLVAAGIDWHPGDNVV
                     MPQEEFPSNVYPFLNLGRLGVELRMVEKDEQGFTSLERVAAAMDGHTRAVVLSHVEFM
                     TGYKNDLDALGRLCQEHGALSVVDATQSMGALPIDVAQSGIDVIAAHGYKWLMASFGL
                     GVVHLSERAIEAIHPVYVGRLSVALGFEELEYRIHWQPGARRYQTGGINWLGVAAFNA
                     SATLIEAAEPARTAAHCLALTDRLLAGVAELGYQVTSCLDPAHRSQIVSFSSGDRDTD
                     ERLVENLEAQQVSTTLRGRGVRVSPYFYNTEADIDRLLELLPPR"
     gene            5830..7314
                     /locus_tag="VFU72_04065"
     CDS             5830..7314
                     /locus_tag="VFU72_04065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEU5422693.1"
                     /translation="MRRLLSAVCLLFSMLMPLGMQAGSSLQPVLMHPTVGDYAFITAS
                     ETPPTEAQCMSVGRRCFTPQAIQAAYNMGPLYAAGNNGQGITIAIVDSYGSDTIAHDL
                     HVFDQAFGLQPMCGEEGVTCASGMPSFSILHVQGAPATKAPPATSKGTGQEDKAAWAL
                     EVALDVEMAHATAPMANILLVTTPTAETLGVQGFPDMMNAEQYVVDHHLAQVISQSFA
                     SAEDAFGSAQSLLNLRHAYVAAAQNGVTVLGSSGDDGTANGAKQPVGQGGSTIPYPTV
                     EWPASDPLVTGVGGTYLCTDPTNTTSRVVDSTDPPGRCGGANTGQAEVGWIVSGGGYS
                     HVFAKPAYQDTLPAGSTAIGDMRGVPDVAFQASATTGALVYLSLPPDGQSGLMCGDNP
                     CSTGWYDIGGTSLSCPQWAGLVAIADQINGGGLGLINPALYRIGADASRYAADFYDVT
                     TGANQIDPSIPGYPATTGWDPVTGLGTPNAANLVPDLVAAAHGH"
     gene            complement(7508..>8335)
                     /locus_tag="VFU72_04070"
     CDS             complement(7508..>8335)
                     /locus_tag="VFU72_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5422694.1"
                     /translation="QDIARKRDLYGRAAALFRQAAPLLRPAIEPVEIPWRDLSLPAYL
                     RCPEGDAGPYPLVVLLNGASTVKEETTRWSRPLLKRGFATLALDTPGSGEAWDRVLGR
                     PGQEDIADALLAFGEAHPALDARRVAVLGISLRGALAVQMAAHDERLAAAISVTAPFH
                     PATYFRHLSELVTGEIAHLTGAESGELSTLVARMSLVDVAPRLRSPLLVIGAGNDLVV
                     PPRESLRLYQAAGGPKRLHFIPKANHVAFSHMEEWLAVAGSWLGEVFAQRGRPALPA"
BASE COUNT         1267 a         2987 c         2909 g         1172 t
ORIGIN      
        1 gaggacgcgt acgaccgcca cgagccgacg cacgccgcgc tgcgccaggc gaccgtcgag
       61 tggttccccc ggctgaagaa cgtcgccttc acgcacgcct ggggcggccc gctcggcatg
      121 ccgcgcgact ggctgccgac gatgtcctac gaccgcgccg ccggcatcgc ctcctcgcgc
      181 ggctacaccg gccagggcgt ggcgacggcc aacctggccg ggcgcgtgct caccgacctg
      241 atcctggagc gcgagacgcc cctgaccgag ctgccgctgg tcaatcacca gtcgccggat
      301 tgggagccgg agccgctgcg ctggctcggg gtgcgctacg tgcagcgcgg ctaccaacgc
      361 ctggacgagc aatccgagcg ctccggccgc gcgcccaacg gccgcaccct ggtcgagcgc
      421 ctgggcagcc actgacgagg gcgagaggtt tgcccattga ggacgttggg tggcgcgtga
      481 ggagcaaggc agccctagat cacgccctcg tcgcgcaggc gggcgatctc gtcgggcgag
      541 aggccgaggc gctcgtgcag caccgcctcg gtctgctccc cgagcagcgg cgggtgaagc
      601 cgcaccgagg ccggagtccc cgagagcgtg aaggggatgc cggccaggcg cacgctgccg
      661 gcggtcgggt gcggcacttc gaccagcatg tcgcggtgca agacctgggg gtgggcgaag
      721 acgcggtcga cggtgttgat cggcccgctg gggatgccgg ccgcgttcat ggccgccagc
      781 cagtcgtcgg cgtcgcgcgt ggcgaagatc tcctccagga gcgccaccag tgtgtcgcgg
      841 ttggccacgc gcgcggggtt ggtggcgtag cgtgcgtcct cggccagctc ggagcgcccg
      901 acgatgccgg ccagcgcgcg gaactggcgg tcattcccca ccgccaccgt catccagcgg
      961 tcgcgcgcgc ggaacgtctg gtagggcacg atgttggggt gggcgttgcc cagccggccc
     1021 ggcaccttgc ccgagatgag gtagttgctg ccgacgttgg ccagccaggc gacggccgag
     1081 gtcaagagcg cggcgtcgat gcgctggccg cgccccgtga gatcgcgcgc gtgcagcgcc
     1141 gccaggatgg cgttcccagc gaatagcccc gtcgttatgt caacgatggc cacgccgacc
     1201 ttggtcggct ccccctccgc ttcgccggtg atgctcatga tgccggcctc gccctgggcc
     1261 acgaagtcgt agccggggtg gtcggcgtag gggccggtct ggccgtagcc ggtgatcgag
     1321 caccagatca ggccggggtt ctcggccgcg agcgcgtcgt agccgatgcc catgcggtcg
     1381 agggcgccga ccttgaagtt ctcgaccacg atgtcgctct gacgcgcgag cgcacgcagg
     1441 atctcgcgcc cgcgctcgct cttgaggttg agtgtgacgc tctgcttgtt gcggttgatg
     1501 ctgaggtaat aggcgctctc gccgccggcc cagggcggcc cccaggcgcg agtgtcgtcg
     1561 ccgctgccgg gctgctcgat cttgatgacc tcggcgccca ggtcgcccag caccatggtg
     1621 caatacgggc cggccagcac ccgcgagaga tcgagcaccc gaatgccgtc gagcggcccg
     1681 cgcgcaccct ccccttcacc ttcaccttcc gccatcacgc cgcctttccg cgcacgccag
     1741 tccatcgggc gccagcctat cacaggggac gtggcagtcg agaagactgg gtgccaggtg
     1801 cggcttggat ccttcgacta cgcccgggat gacagggtga gagaggggct gacgaggagg
     1861 atgcgtcttg ggcgatgcgc ttcctccccc ggtaggcccc aacacttccc ctgtcatcct
     1921 gagcttgtcg aaggatccaa cctggagcgg cacgcaacgc gttcgaccgc cccgcgccgc
     1981 tccactggca ttccggggcc tcagaggctg atgaccgagt cctcgctgcc gaagcggttc
     2041 ggctcgtgcc ggtcggcggc ggtgtcgttg acccagcgat cgccgtcgag cagatagcgg
     2101 aagcggtagt cggtccccgg tttgagcgag atggtggtgg cgaagctgcc gtccttgcgc
     2161 cgcttgagct ggcgcgcggc cggatcccaa tcattgaaat cgcccagcag gctcaccgac
     2221 tccgcgtcga cctcgcacgg cagctcgaag gtcacgcggc acgagcggcc ggtcttggtg
     2281 tactccttga cgatcaacgg tgcgaatccc ttctcaacac acagcatgat ccggccaggt
     2341 gaaccaggat ggttcgcttc cgcgccggtg gccgcaagtc tgcctgagaa gggtaccgtg
     2401 atgcaaacca aatgcgccct gggccgcgcg gccagtcagg ccggcgatgg tgcgctcacg
     2461 cccgcggcgg gggcccggcg cggccgtcaa ccctcgtccg gccgcagctc cttgaccagc
     2521 ttgagcgttt cctcctggta gccgagccgc cggtagaagg cgcgggcgcg ctcgttggcg
     2581 gcgaagacct ccagcgccag caaccggtag ccccgggacc gcgcccactc ctcggccgcc
     2641 gccatcagcg cccggccgac gccctgcccc tcggcctcgg cggccaccgc cagcgtggcg
     2701 aggtagccct gcgcttcgcc gctcaacccg ctctccgcgc cggtcacgtg gaccacgccc
     2761 agcggtctgc ccgccgcgtc ctcggcgatc agggccagct gctccggacc ctcagtcgcc
     2821 agcagcgcct cggcgtagcg acggtggaag gcccgcatcg cctgcggttc gcgccagggc
     2881 ggcataccgg cggcctccag ccgcgcggcc agcgacagca cgaagtcgcg atccgcgggt
     2941 cgcagcggcc ggcagcggag gactccacgt tcctgctggc tcatcctggc tccaatcaac
     3001 cgtgcgtaac gcacaacacg ggctcgtccc ggggctggta tggcaaacgg ggcgagctgg
     3061 cctcgcccca ctcattatgc cgcgtgctgc atctagggac gcgcgctagg ggaactcggg
     3121 cacatcgtcg tgctgcgcgt cgtagtccgc cagcatctgt ttgacctgct tggtcgcgtc
     3181 cgaggccgcc gtcgagccct tggagcaggc cgcgccattg accgcgtcgg tgacgagatg
     3241 cgcgttggcg aggatcatgc ccatctgcgc ccgctgatgc gccgccatcg ccgggaagaa
     3301 ggccccccca gcacgcgttc ggcccaggcg accagggctt gcaggtaggc gcgcaggcgc
     3361 tcgcggatct cggcgtcggt caggctgccg gcagcccgga cgctagcgcg ccggcggggc
     3421 ctccagcgtc cggcgctcgg cgaatctgcg cccaaaccag agcggcgcca gcgccagcgc
     3481 ggccaggtag acgaaatagg cgccggtgtc gaacgcgatg ccggccgcgt tggccggccc
     3541 cgggtcggcg gtcttgggcg gttggtagcc cagcagccag agggccgcga agccggccga
     3601 gacgatcagc gcggaggcga gccagatcac ccaggtgcgg cgcgggcgga agtagtagcc
     3661 gctgccgaac gccaccaggg ggagcagcaa ctgcaacggg tcgggcagca gcgccagcga
     3721 gaagaccacg ctgcttatca gcacggtcgg aatcacggag cgcgtgagtg cgtcggcgct
     3781 cgccagcctg gttgacatgc gttcctcctg tacccacgcc cggtacttag tgcggcgaga
     3841 gcacctcgcc cagggcaaag gcggcgacga agagcgcgta gacgatgacg ggatagagca
     3901 ggatcgagcg ctcgcgccgc cggatgagcg cgaagagcgc cagcggaccg gccgcgatcg
     3961 ccgtcgctcc ggccagcagg atgaacgcgg cgtgccaggg attgctgaaa aaggtctcgc
     4021 ccccgccgcc cgcgccgaaa cgcggcccca ggcgccagag gagcatgaag aagacatgca
     4081 ggcccagcag cgccagggcg cccagaccgg cgcgcgtgcg cggcgcgctg aagagccggc
     4141 tcatgagcgg ctccccggtg gactcgtgcg tgccggccat ctcgacaccc cccgctgatc
     4201 accacacggc cgtcacgggt actctagacc acggcgcgtc acgggactgt cgcagcaccg
     4261 gcccggtcat gtcaggatcg taacagtgcg gccggcgcgc cctgcccagc acgcggagga
     4321 tcgagacagc gcgggcgaag cgcgctagca tgagaccgcc gaggcgctcg gcacggttgc
     4381 tggaaggatg atgagcgatg actgtcgagc tggcgacgct ccgggcggag ttcccaatcg
     4441 ccgaggagtg ggcctacctg aatcacgcgg cgatcggccc cttctcgcgc cgcaccgagg
     4501 cggcaatcgc ggcggtgagc ggcgccttcg cccggccacc ttccatgggc aacgcctacg
     4561 gcgaggcgat cgagacggcg cgcggcaacg tggccaaatt cgtcggcggc acgcccgacg
     4621 gcgtggcctt cgtgggcagc ctggccgacg ccatgagcct ggtcgcggcc gggatcgact
     4681 ggcatcccgg cgacaacgtg gtcatgccgc aggaggagtt cccctccaac gtctacccct
     4741 tcctcaacct gggccggctg ggcgtcgagc tgcgcatggt cgagaaggac gagcagggct
     4801 tcaccagcct ggagcgcgtg gcggccgcga tggacggcca cacgcgcgcg gtcgtcctca
     4861 gccacgtcga gttcatgacc ggctacaaga acgacctcga cgccctgggc cggctctgcc
     4921 aggagcacgg cgcgctctcg gtcgtcgacg ccacccagtc gatgggcgcg ctgccgatcg
     4981 acgtggccca gagcggcatc gacgtgatcg ccgcgcacgg ctacaagtgg ctgatggcgt
     5041 cctttggcct gggcgtggtg cacctctccg agcgcgccat cgaggcgatc cacccggtct
     5101 acgtcggccg cctgagcgtg gcgctcggct tcgaggagct ggagtaccgc atccactggc
     5161 agccgggcgc gcgccgctac cagaccggcg gcatcaactg gctcggcgtg gccgcgttca
     5221 acgcctcggc cacgctgatc gaggcggccg aaccggcgcg caccgcggcg cactgcctgg
     5281 cgctgaccga ccggctgctc gcgggcgtgg ccgagctggg ctaccaggtg acctcctgcc
     5341 tcgaccccgc tcaccgctcc cagatcgtga gcttttccag cggcgaccgc gacaccgacg
     5401 agcgcctggt cgagaacctg gaggcgcagc aggtctcgac caccctgcgc ggccgcggtg
     5461 tgcgcgtctc accgtacttc tacaacacgg aagccgacat cgaccgcctg ctggagctgc
     5521 tgccgccgcg ttagcctccg cgcgaggacc gcgccggaaa ccagcgcggt cctcgcgtta
     5581 atcttcgaac ccgcctcacg catcgacgcc attcaacaac ctgctatgat gccgacctat
     5641 gccgcggaat cgctcgcctc acgaggtata gtgggccgcg gcgtgggtgc cggcgtcgat
     5701 ccgtcgacca aacgaggagg aggttgcggg cgaagtcttc tgcgagtcgg gccccgcgtg
     5761 cgagcttgcc tcgcgggcgc gggggtggcg tgccgcgcgc acgcgttcac gaggaagagg
     5821 gagagcgaga tgcgtcgttt actctccgcg gtgtgtcttc tcttcagcat gctcatgccc
     5881 ctcggcatgc aagccggctc atcgctgcag ccggtgctga tgcaccccac cgtcggcgac
     5941 tacgccttca tcacggcgtc ggagacgccg ccgaccgagg cccagtgcat gtcggtcgga
     6001 cggcgctgct tcacgccaca ggcgatccag gcggcctaca acatgggtcc gctctacgcc
     6061 gcgggcaaca acgggcaggg catcaccatc gccatcgtcg actcctacgg cagcgacacg
     6121 atcgcgcacg acctgcacgt cttcgatcag gccttcggtc tgcagccgat gtgcggtgag
     6181 gaaggtgtga cctgcgccag cggcatgccc agcttcagca tcctgcacgt gcagggggcg
     6241 ccggctacca aggcgccgcc cgcgaccagc aagggcaccg ggcaggagga caaggcggcc
     6301 tgggcgctgg aggtcgcgct cgacgtcgag atggcgcacg ccacggcgcc aatggccaat
     6361 atcctcttgg tcaccacgcc caccgccgag acgctcggcg tgcagggctt ccccgatatg
     6421 atgaacgccg agcagtacgt ggtcgaccat cacctggccc aggtcatctc gcagagcttc
     6481 gcctcggccg aggatgcctt cggcagcgcc cagtcgctgc tgaacctgcg gcacgcctac
     6541 gtcgccgcgg cgcagaacgg cgtcaccgta ctcggctcct cgggtgacga cggcaccgcc
     6601 aacggcgcca agcagccggt cggccagggc ggctcgacca tcccctatcc caccgtcgag
     6661 tggcccgcca gcgatccgct ggtgaccggc gtcggcggca cctacctctg caccgacccg
     6721 accaacacga ccagccgcgt ggtcgatagc accgacccac ccggccgctg cggcggcgcc
     6781 aataccggcc aggccgaggt cggctggatc gtcagcggcg gcggctacag ccacgtcttc
     6841 gccaagccgg cctaccagga cacgctgccg gccggcagca cggcgatcgg cgacatgcgc
     6901 ggcgtgcccg acgttgcttt ccaagccagc gccacgaccg gcgcgctcgt ctatctgtcg
     6961 ctcccgccgg atggccagag tgggctgatg tgcggcgata acccgtgcag caccggctgg
     7021 tacgatatcg gcggcacctc gctgagctgc ccgcagtggg ccgggctggt ggcgatcgcc
     7081 gatcagatca acggcggcgg tctgggcctg atcaaccccg cgctctaccg catcggggct
     7141 gacgcctccc gctacgccgc cgacttctac gacgtgacca ccggcgccaa ccagatcgat
     7201 ccgtcgatcc ccggctaccc ggcgacgacc ggctgggatc cggtgaccgg tttgggcacg
     7261 cccaacgcgg ccaacctcgt ccccgatctc gtcgccgccg cgcacggcca ctgagccgcg
     7321 cacgctcgcg gtgacaatcg cggcccggcc atccaggccg ggccgtttcg ctgcctcacg
     7381 atgaagtttt atgtcgagtt ggtgtcttgt gaggcagcgg tctgcgcctg aaagcgaacg
     7441 ctgggagccg cgtgaggacg ggtccttcgg ctgcgccatg ccttgtcgcc ggccacccct
     7501 ccccgcccta cgcgggcagc gcggggcgac cgcgctgggc gaagacctcg cccagccagg
     7561 agccagccac cgccagccat tcctccatgt gcgagaaggc gacgtggttg gccttgggga
     7621 tgaagtgcag ccgcttgggg ccgccggccg cctgatagag ccgcagcgac tcgcgcggcg
     7681 gcaccaccag atcgttgccg gcgccgatca ccagcagcgg cgaccgcagc cgcggggcga
     7741 cgtcgaccag cgacatgcgt gcgaccagcg tgctcagctc ccccgactcc gctccggtca
     7801 ggtgcgcgat ctcgccggtg accagctcgc tgagatgccg gaaatacgtc gcggggtgga
     7861 acggcgcggt gaccgagatc gcggcggcca ggcgctcatc gtgcgcggcc atctgcaccg
     7921 cgagagcgcc gcgcaggctg atgccgagca cggcgacgcg gcgcgcgtcg agcgccgggt
     7981 gcgcctcccc aaaggccagc agcgcgtcgg cgatatcctc ctgaccgggg cgccccagca
     8041 cgcggtccca ggcctcgccg ctgccgggcg tgtcgagcgc cagcgtggcg aagccgcgct
     8101 tcaggagcgg ccgcgaccag cgcgtcgtct cctccttgac cgtgctggcg ccgttgagca
     8161 ggaccaccag cgggtacggc ccagcgtcac cctccgggca acgcaggtag gccggcaggc
     8221 tgaggtcgcg ccaggggatc tcgaccggct cgatggccgg gcgcagcagc ggcgcggcct
     8281 ggcggaagag cgcggcggcg cgcccgtaga ggtcgcgctt gcgggcgata tcctg
//
LOCUS       DASUBI010000209         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_452183, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000209 DASUBI010000000
VERSION     DASUBI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452183"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFU72_04075"
     CDS             <1..1194
                     /locus_tag="VFU72_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU5422695.1"
                     /translation="CSPSRAALFTGRYPHSNGVLGLTHANFAWDLHPGERHLGQVLQA
                     AGYATALFGVQHESLRGSDAEVAASCGIDDIALGGRGEEVSDRALAWLAQGGRADRPF
                     FLELGYQEPHRLRATTHDEPDYHGFVGDYLAADDTRGVTVPPYLRDTPLARQELAELQ
                     GAVRYVDAQIGRVLAGLRDLGLDEHTLVVMTTDHGVALPRAKCSLYDPGLETALILRL
                     PARGWRGGRLVDALIANVDIFPTVLDAAGLPAPDNVQGRSLLALLDGAAGYIPRDHLF
                     AEMTYHDYYDPRRCIRTEQYKLIVNFSAAPGFMDPSQSWRPRTDTVVPAHPALDYHPL
                     VELYDPVADPLEWHNLADDPAYAIVRADLLHRLGRWMRETADPLLQGAVTSPLHQRAV
                     AALVD"
     gene            1353..2624
                     /locus_tag="VFU72_04080"
     CDS             1353..2624
                     /locus_tag="VFU72_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422696.1"
                     /translation="MSRWRRTLYTIFATQVLTLIGFSCVFPFFPLYIQTLGIAGPAVI
                     LWSGVITFSGSFTLAVASPIWGALADRYGRKPMLIRAMTGGAVVTALLIVAPNIWVVL
                     ILRVLQGTLTGTVAPARALVAAVTPREELGFSMGLMEASIFLGSAVGPFAGGFLNDRL
                     GFHGTFAVGSSLLAISAILVLFLIDEQFTPAASAGHHTLNPVANLRQTALMPGLAILA
                     LVLFGANFANSVPSPVLPLLIPELHGVPTIGGVPQVGTAVGLVLAVAGLCATAAAWRT
                     RLLTNRFGYRRTLIGALIVAGAFTVPAAIVGTVWQMLAVRAAVGLCIGAALPTVSALV
                     SLMTPRGQQGSAYGLLASAELIGFALGPLVGGVIGASLGLRATFLVPAAVLILVAMLV
                     LTRVHIPPTAEITSVPHGAEPLAEEEVTPAT"
BASE COUNT          372 a          969 c          924 g          492 t
ORIGIN      
        1 tgcagcccct cgcgcgccgc gctcttcacc gggcgctatc cgcacagcaa cggcgtgctc
       61 ggcctgacgc acgcgaattt cgcctgggat ttgcatcccg gcgagcgcca tctcggccag
      121 gtgttgcagg cggccggcta cgcgacggcg ctcttcggcg tgcagcacga gtcgttgcgc
      181 gggagtgatg cggaggttgc cgccagctgc ggcattgacg acatcgccct cggcgggcgc
      241 ggcgaggagg tcagcgaccg tgcgctggcg tggctggcgc aaggtggacg cgctgaccgg
      301 cccttcttcc tcgaactggg ctaccaggag ccgcatcgcc tgcgcgccac cacccacgac
      361 gagcctgatt atcacggctt tgtgggcgat tacctcgcgg ccgacgacac ccgcggcgtc
      421 accgtgccac cctacctgcg cgacacgccg ctggcgcggc aggagctggc cgaactgcaa
      481 ggcgcggtgc gctacgtaga cgcccagatc ggccgtgtac tggccgggtt gcgcgacctc
      541 ggcctggacg agcacacgct ggtcgtgatg acgaccgacc atggcgtggc cctgccgcgc
      601 gccaagtgct cgctctacga tcctggcctg gagacggcgc tgatcctacg actgcccgcg
      661 cgtggctggc gcggtggccg tttggtggac gccctcatcg ccaacgtgga catctttccg
      721 accgtgctcg atgcggctgg gctgccggcg ccggacaatg tgcagggtcg cagcctgctg
      781 gcgctgctcg atggcgcggc tggctacata ccgcgcgacc atctcttcgc cgagatgacc
      841 tatcacgact actacgatcc ccggcgctgc atccgcaccg agcagtacaa gttgattgtc
      901 aacttcagcg ccgcgccggg gttcatggat ccgtcgcagt cgtggcggcc acgcaccgat
      961 acggtcgtgc ccgcgcaccc ggcgctcgac tatcatccgc tggtcgaact gtacgacccc
     1021 gtggccgacc cgctggagtg gcacaacctc gcggacgatc cggcatatgc tatcgtgcgc
     1081 gccgatctgc tgcaccggct aggtcgctgg atgcgcgaga ccgccgatcc actcctccag
     1141 ggcgcggtca cgtcgccact gcaccagcgc gcggtggcgg cgctggtcga ctgagtgaca
     1201 ggccggttcg ggctggacga gcgacgcggc tttccccgct aacgggcaca atcttacctg
     1261 ccttgctaca ctgctcgcgg cggcgctgcc cggcatcgtt cactcgcggc cgacggatgt
     1321 gattcgactg acgaccatgc gactgagggg acatgagccg ctggcgccgc acgctctaca
     1381 cgatcttcgc gacgcaggtg ctcaccctga tcgggttcag ctgcgtcttt ccgtttttcc
     1441 cgctctatat tcagacactc ggcatcgccg gccccgccgt catcctctgg tcgggcgtca
     1501 tcaccttcag cggctcgttc acgctcgccg tggcctcgcc gatctggggc gcgctggccg
     1561 atcggtatgg ccgcaagccg atgctcatcc gcgccatgac cggcggcgcg gtagtgaccg
     1621 cgctgctgat cgtcgcgccg aacatctggg tcgtgctgat attgcgcgtg ctgcagggca
     1681 cgctcaccgg cacggtcgcg ccggcccgcg cgctggtcgc agccgtcaca ccgcgcgaag
     1741 agctcggttt cagcatggga ctgatggagg catccatttt cctcggcagc gcggtcggcc
     1801 ccttcgctgg cggctttctg aacgaccggc ttggcttcca cggcaccttc gccgtaggct
     1861 cgtcgctgct ggcgatctcc gccatcctgg tgctcttcct gattgacgag cagttcacgc
     1921 cagcggccag cgcgggccac catacactga accccgtcgc caacctgcgc cagacggcgt
     1981 tgatgccggg actggcgatc ctggcgctgg tgctattcgg cgcgaacttc gccaactcgg
     2041 tgccgtcgcc ggtgctgccg ctgctcattc ccgaactgca cggcgtaccg accatcggcg
     2101 gcgtgccgca ggtcggcacg gcggtcggcc tggtgctggc ggtcgcgggc ctctgcgcca
     2161 ccgccgccgc ctggcgtacg cgcctgctca ccaaccgctt cggctatcgc cgcaccctga
     2221 tcggcgcttt gatcgtggcc ggcgcattca ccgtgccggc ggcgattgtc ggcaccgtct
     2281 ggcaaatgct ggccgtccgc gcggcggtgg gtctctgtat cggcgcggcg ctgccgacag
     2341 tcagtgcgct cgtcagcctg atgaccccgc gtgggcagca ggggtcagcg tatggcctgc
     2401 tggcgtcggc ggagttgatc ggcttcgcgc tcggcccgct ggtcggcggg gttatcgggg
     2461 cctcgctcgg cctgcgcgcg acctttctgg tgccggccgc agtgctgatc ctcgtggcaa
     2521 tgctggtgct gaccagggtg catattccgc caacggcgga gataaccagt gtgccgcacg
     2581 gcgcggagcc gctggctgag gaagaagtca cgcccgctac atagctgccg caagcgcggt
     2641 ggcgctgcgg ggttgcgccg aatgaattcg gcgcgaaggg cggctccgcc gccacgaagt
     2701 ccctgcggga ctggggctgg tctaccactg tgccagcagc tagcctcggc ctcgcaa
//
LOCUS       DASUBI010000210         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_889861, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000210 DASUBI010000000
VERSION     DASUBI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889861"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFU72_04085"
     CDS             <1..255
                     /locus_tag="VFU72_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422697.1"
                     /translation="AKLAVIYPRLTNTASMADYWLPAAPGTEAALLLGIAHVMLRDDL
                     CDLDFVRRWTNWDEYLRVVHPNEPIEFDRFLALLTEEYAR"
     gene            286..2166
                     /locus_tag="VFU72_04090"
     CDS             286..2166
                     /locus_tag="VFU72_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU5422698.1"
                     /translation="MPGATIVELAREIGAAGSAFSSHNWRAAAAGHLGGWEVPRTLFL
                     LNVLSGSVGTPGGVAANVWDKFVPRPFAEPAKQKVWNEVSWPLEYPLAHHEMSFLLPH
                     FLKEGRGKLAMYFTRVYNPVWTNPDGFVWIDALKNESLIELHAALTPTWSESAWFADF
                     VLPMGVGAERHDSFSYETHAAKWIGFRQPVVRVAMERLGQKVEFTYQANPGEVWEEDE
                     FWIELSWRIDPDGSLGIRKFYESPYRLGEKITIDEYYQWLFENSVPGLPEAAAAEGLT
                     PLGYMRRYGAFEIAKQPSRPYDEHVPLEALEGAGVDPVSGVIMSPSPRPHGPEITPLP
                     GNPPSAEGRAVGVLIDGEPCLGFPTPSGKLEFFSRTLHDWGWPEYAIPSYIPSQVAPS
                     EIDVEAGEMDLLPNYRIPTLVHTRTGNSKWLNEISHSNPLWLHPSDAGRIGVKTGDLA
                     RVETEIGYFVDRVWVTEGMRPGVVACSHHLGRWRLDEARGMDRWGSALVRLEEPEPGV
                     YRMRWREGVHPFVSSDPDTLNVWWSDAGVHQNLTFPVQPDPISGSHAWHQKVKVVAAK
                     ETDRYGDIVVDTEKSMAAYRRWLELARPAPGPGNMRRPLWLLRPYKPDPSAYQVGGPT
                     GR"
BASE COUNT          339 a          794 c          772 g          330 t
ORIGIN      
        1 gccaagctgg cggtgatcta cccgcgcctc accaacaccg cctcgatggc cgactactgg
       61 ctgccggccg cgccgggcac cgaggcggcg ctgctgctgg gcatcgccca tgtcatgctg
      121 cgcgacgacc tctgcgatct cgacttcgtg cgccgctgga ccaactggga cgagtacctg
      181 cgcgtggtgc accccaacga gccaattgag ttcgatcgct tcctggcgct gctcaccgag
      241 gaatacgcgc gctagacgcc ggagttcgtc gaggccgaat gcggcgtgcc gggggcgacc
      301 atcgtcgagc tggcgcgcga gatcggcgcg gcgggctcgg ccttctcctc gcacaactgg
      361 cgcgcggcgg ccgccggcca tctcggcggc tgggaggtgc cgcgcacgct gttcctcttg
      421 aacgtcctct ccggcagcgt cggcacgccg ggcggggtgg cggccaacgt ctgggacaag
      481 ttcgtgccgc gccccttcgc cgagccggcc aagcagaagg tctggaacga ggtctcctgg
      541 ccgctggagt acccgctggc gcaccacgag atgagcttcc tcttgccgca cttcctcaag
      601 gaggggcgcg gcaagctggc gatgtacttc acccgcgtct acaacccggt ctggaccaac
      661 ccggacggct tcgtctggat cgacgcgctc aagaacgagt cgctgatcga gctgcacgcc
      721 gcgctgacgc cgacctggag cgagtcggcc tggttcgccg acttcgtgct gccgatgggc
      781 gtcggcgccg agcggcacga ctccttctcc tacgagacgc acgccgccaa gtggatcggc
      841 ttccgccagc cggtggtgcg ggtggcgatg gagcggctgg ggcagaaggt cgagttcacc
      901 taccaggcca accccggcga ggtctgggag gaggacgaat tctggatcga gctctcctgg
      961 cgtatcgacc ccgacggctc gctcggcatc cgcaagttct acgagtcgcc ctatcgcctg
     1021 ggcgagaaga tcaccatcga cgagtactac cagtggctct tcgagaactc ggtgccgggg
     1081 ctgcccgagg ctgccgccgc cgaggggctg acgccgctcg gctacatgcg ccgctacggc
     1141 gccttcgaga tcgccaagca gccctcccgc ccctacgacg agcacgtgcc gctggaggcg
     1201 ctggagggcg ccggtgtcga ccccgtgagc ggggtcatca tgagccccag cccgcgcccg
     1261 cacggccccg agatcacgcc gctgccgggc aacccgccgt ccgcggaggg gcgcgcggtg
     1321 ggcgtgctga tcgacggcga gccctgcctc ggcttcccga cgccctccgg caagctggag
     1381 ttcttctcgc gcacgctgca cgactggggc tggccggagt acgccatccc cagctatatc
     1441 cccagccagg tggcgccgag cgagatcgac gttgaagcgg gcgagatgga cctgctgccc
     1501 aactaccgca tcccgacgct ggtgcacacg cgcaccggca acagcaagtg gctcaacgag
     1561 atcagccact ccaacccgct ctggctgcac ccgtcggacg ccgggcgtat cggcgtcaag
     1621 actggcgacc tggcgcgcgt cgagactgag atcggctact tcgtcgatcg cgtctgggtg
     1681 accgagggga tgcgccccgg agtggtggcc tgctcgcatc atctggggcg ctggcgcctg
     1741 gacgaggcgc gcggcatgga ccgctggggc tcggcgctgg tgcggctgga ggagccggag
     1801 ccgggcgtct accgcatgcg ctggcgcgag ggggtacatc ccttcgtcag cagcgacccc
     1861 gacacgctga atgtctggtg gagcgacgcc ggggtgcacc agaacctgac cttcccggtg
     1921 cagcccgacc cgatcagcgg ctcgcacgcc tggcaccaga aggtgaaggt ggtggcggcc
     1981 aaggagaccg accgctatgg cgacatcgtg gtcgacaccg agaagtcgat ggcggcctac
     2041 cgccgctggc tcgaactcgc ccgcccggcc cccggccccg gcaacatgcg ccggccgctc
     2101 tggctgctgc gcccctacaa gcccgacccc agcgcctatc aagtcggggg gccgacggga
     2161 cggtagggcc ctcaccccct cccctctccc actgcggcgg gggaggggcg ctgcccatcg
     2221 acgaggatga gtgcc
//
LOCUS       DASUBI010000211         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_54602, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000211 DASUBI010000000
VERSION     DASUBI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54602"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFU72_04095"
     CDS             complement(<1..575)
                     /locus_tag="VFU72_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422699.1"
                     /translation="MATDNPAARLGHVLWIGGPPDSGKTSIADALAVRYGLQVYHFDR
                     QEPVHIGRADPARQPALTRLRTLLETLDERAFAEALWLAQEPDEMAAWTIASWSERFA
                     MARDDLLAMPESPGIVAEGPGFFPECVAPLLDDPRKAIWLVPDEDFKRASVARRGKPG
                     ARRHTSDPERASRNLTARDLLLGRHVVAQAQA"
     gene            complement(659..2284)
                     /locus_tag="VFU72_04100"
     CDS             complement(659..2284)
                     /locus_tag="VFU72_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5422700.1"
                     /translation="MTGGQALVASLIKQGVDTVFGLPGVQLDWLFDAIWEKRDQIKVY
                     YTRHEQGTAYMADGYARTTGKVGTCIVVPGPGLLNAMAGLATAYSASSPVLCVTGQIR
                     SDAIEQGRGLLHEIPNQLDMVRSATKWAARAMTPAEVPGVVEEAFRQLRSDRVRPVEI
                     EVPPDILQASGQVTIPGRVAEQRLQGDPELLDKAAKLLGEAQRPVIIAGGGVIRSGAS
                     DELKALAEMLQAPVVMSSHGKGSLSYRDDLALNPNALDELVPGADVVLLVGNRYVEYG
                     INEWWRPAKGQVGIRLDTDAEMLERSQPTLGILTDAMLGLAELVERVGKYNHAREPRT
                     AELKALKAHLEDLWWEVQPQAAYATVLREELPDDGILVSESTQVGYWSDRAFPVYEPR
                     TYLTSGYQGTLGYGYATALGAQVGNPDRKVVSINGDGGFMYNVQELGTAVQQGINAVG
                     VVFNDRAYGNVRRTQRLNFNGHIIATDLHNPDFVKLADSFGMAGIRAEGPEGLRAALR
                     EALALKGPALIEVPVNEMPNIRTTLQSAQARTR"
     gene            2365..>3390
                     /locus_tag="VFU72_04105"
     CDS             2365..>3390
                     /locus_tag="VFU72_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5422701.1"
                     /translation="MRIETPLAAVIFTLTLLLVLARPRELAEAWWAAAGGLLVLLLGL
                     VTPQQAWRILRLAEDALVFLIGMMILSALAERAGFFEWTAGLAARAGGGKVLRLYIFV
                     FLVGAVITATLSLDATAIVLTPIIYGMVVRLRLRPLAFMFAISAIANTASLFLPISNL
                     TNLLLYGALGLDFFHFALVMLLPALLAVLTNIVVFILIFRHDLRGGYPREAGGFAPKD
                     RAFFRLASIWLGLVLAGVFTVSALGLPVGPVALGGGLLLLAVSWARGWAAPGPVLRAV
                     SWELIVLVLGLFLVVQGVENAGLAGLIQRAFDAAAPGAQLLQIAATALGTTLGANLIN
                     NIPMTIVT"
BASE COUNT          423 a         1180 c         1213 g          574 t
ORIGIN      
        1 gcctgagcct gcgcgacgac gtgccggccc agcagcaggt cgcgcgcggt caggttgcgg
       61 ctggcgcgct cggggtcgct ggtgtggcgc cgggcgccgg gcttgccgcg ccgggccacc
      121 gaggcgcgtt tgaaatcctc gtccggcacc agccagatcg ccttgcgcgg gtcgtccaag
      181 agcggggcga cgcactcggg gaagaagccc ggcccctcgg ccacgatgcc gggcgactcc
      241 ggcattgcca gcagatcgtc acgcgccatg gcgaagcgct ccgaccaact ggcgatggtc
      301 cacgccgcca tctcgtccgg ctcctgcgcg agccacagcg cctcggcgaa ggcgcgttcg
      361 tcgagcgtct ccagtagcgt gcgcagccgg gtcagggccg gctggcgcgc ggggtcggca
      421 cggccgatgt ggaccggctc ctgacgatcg aagtggtaga cctgcaggcc gtacctgacg
      481 gccagcgcat cggcgatcga ggtcttgccg ctgtcgggcg gcccgccgat ccagaggacg
      541 tggcccagcc gcgcggcggg attatccgta gccatcgcga cgctcctttg cgggtgcgat
      601 cggcgcgaac ggcgaagcgc cccaccgccg tttggcagcg gggcgcgttc gatacgcgct
      661 agcgggtgcg cgcctgcgcc gactggagcg tggtgcggat gttcggcatc tcgttgaccg
      721 gcacctcgat cagcgccgga cccttgagcg ccagcgcctc gcgcagcgcg gcgcgcaggc
      781 cctccggccc ctcggcccgg atgccggcca tgccgaagga gtcggccagc ttgacgaagt
      841 ccgggttgtg gaggtcggtg gcgatgatgt ggccgttgaa gttcagccgc tgggtgcggc
      901 gcacgttgcc gtaggcacgg tcgttgaaga ccacgccgac ggcgttgatc ccctgctgca
      961 ccgcggtgcc cagctcctgc acgttgtaca tgaagccgcc gtcaccgttg atcgagacga
     1021 ccttcctgtc cgggttgccg acctgggcgc cgagcgcggt ggcgtagccg tagccgagcg
     1081 tgccctggta gccgctggtg agataggtgc gcggctcgta gaccgggaag gcgcggtccg
     1141 accagtagcc gacctgggtg ctctcgctga ccaggatgcc gtcgtcgggc agctcctcgc
     1201 gcagcacggt ggcgtaggcg gcctgcggct gcacctccca ccagaggtct tccaggtgcg
     1261 ccttgagcgc cttcagctcg gcggtgcgcg gctcgcgcgc gtggttgtac ttgccgacgc
     1321 gctcgaccag ctcggccagc ccgagcatgg cgtcggtcag gatgccgagg gtcggctggc
     1381 tgcgctccag catctcggcg tcggtgtcga gccggatgcc gacctgcccc ttggccgggc
     1441 gccaccactc gttgatgccg tactcgacgt agcggttgcc gaccagaagc accacgtcgg
     1501 cgcccggcac gagctcgtcg agggcgttgg ggttgagcgc caggtcgtcg cggtaggaga
     1561 gggagccctt gccgtgcgag ctcatcacca ccggcgcctg gagcatctcg gccagcgcct
     1621 tcagctcgtc ggatgcgccc gagcggatca cgccgccgcc ggcgatgatc accggccgct
     1681 gggcctcgcc cagcagcttg gcggctttgt ccagcagctc ggggtcgccc tgcaggcgct
     1741 gctcggccac ccgcccgggg atggtcacct ggccgctcgc ctgcaggatg tccggcggca
     1801 cctcgatctc gaccgggcgc acgcggtcgc tgcgcagttg gcggaaggcc tcctcgacga
     1861 cgccgggcac ctcggccgga gtcatggccc gggcggccca cttggtcgcc gagcgcacca
     1921 tgtcgagctg gttggggatc tcgtgcaaga gcccccgccc ctgctcgatg gcgtcggagc
     1981 ggatctgccc ggtgacgcag aggaccggcg agctggccga gtaggcggtc gccagcccgg
     2041 ccatggcgtt gagcagcccg gggccgggca cgacgatgca ggtgccgacc ttgccggtgg
     2101 tgcgggcata gccgtcggcc atgtaggccg tgccctgctc gtggcgggtg taatagacct
     2161 tgatctggtc ccgcttctcc cagatggcgt cgaagagcca gtcgagctgc acgccgggca
     2221 gcccgaagac ggtgtcgaca ccctgtttga tcagcgatgc gacgagggcc tgacccccgg
     2281 tcatctcagc cacgcgtgat cctttcttgc gttcgacctt taccagcccc ggcattgtac
     2341 ggcaatgcca cgtatcatgc gcgcatgcgc atcgagacgc cgctggcggc ggtcatcttc
     2401 acgctcacgc tcctgctggt gctggcccgt ccgcgtgagc tggccgaggc ctggtgggcg
     2461 gcggccggcg ggctgctggt gctgctgctg ggattggtga cgccacagca ggcctggcgc
     2521 atcctccggc tggccgagga cgcgctcgtc ttcttaatcg gcatgatgat cctgagcgcg
     2581 ctggccgaac gcgccggctt cttcgagtgg acggccgggc tggcggcgcg ggccggcggc
     2641 gggaaagtgc tgcggctcta catcttcgtc ttcctggtcg gcgcggtgat caccgccacc
     2701 ctctcgctcg acgccaccgc catcgtgctg acgccgatca tctacggcat ggtggtgcgg
     2761 ctgcggctgc gcccgctggc cttcatgttc gctatcagcg ccatcgccaa caccgcctcg
     2821 ctcttcctgc cgatctcgaa cctcacgaac ctgctgctct acggcgcgct ggggctcgac
     2881 ttcttccact tcgccctggt catgctccta ccggcgctgc tggcggtgct gaccaacatc
     2941 gtcgtcttca tcctcatttt ccgccacgac ctgcgcggcg ggtatccgcg cgaggcgggc
     3001 ggcttcgccc ccaaagaccg cgcgttcttc cggctggcga gcatctggct gggtctcgtg
     3061 ctggccgggg tctttacggt ctcggcgctc ggactgccgg tcgggccggt ggcgctgggc
     3121 ggcgggctgc tcttgctggc ggtcagttgg gcgcgcggct gggcggctcc cgggccggta
     3181 ctgcgcgcgg tctcctggga gctgatcgtg ctggtgctcg ggctcttcct ggtggtgcag
     3241 ggggtggaga acgccgggct ggccgggctg atccagcgcg ccttcgacgc cgccgcgccc
     3301 ggcgcccagc tgctgcagat cgccgccacc gcgctcggaa cgaccctcgg cgccaacctg
     3361 atcaacaaca tcccaatgac gatcgtgaca
//
LOCUS       DASUBI010000212         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1135931, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000212 DASUBI010000000
VERSION     DASUBI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135931"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1555)
                     /gene="rfaE2"
                     /locus_tag="VFU72_04110"
     CDS             complement(56..1555)
                     /gene="rfaE2"
                     /locus_tag="VFU72_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU5422702.1"
                     /translation="MAGAIETVRGFRRLRALVIGDAMLDSYLEGTAARLCAEGPVPVV
                     RKTAEERLPGGAANTAANLAALGAEVLFLGVVGADPAGALLRAALRDHDVDDSWLVED
                     DAISTLHKLRILANGQYVVRFDDGGAESLGHRTYERLLATLDHAFPRCDLVVISDYNY
                     GVLSDGLVRHLRALRQARPCVLAVDSKHLRRFRESDATIITPNHLEALLAVDGAVPAG
                     EGLNLHEVKRAGRRLVEAIETEHVAVTLGAGGVCLLDRRGRARHFPAHPVANPSDVGA
                     GDSFLAALALALASGARCTEAIQIGIEAAGIAVSKRRTAVVLYQELLQRISLREHLPR
                     AGDLDVFGAVAAQLDAARERGRKIVFTNGVFDILHAGHVEFLRRARALGDVLVVGVNT
                     DRSARLLKGKNRPINSEEHRLALVAALDAVDHALLFDEETPEALIRDLRPHIHVKGGD
                     YAGEDLPEAEAVAEVGGRVVILPLVGDLSTSGVIDRIVALAGDGLGGER"
     gene            complement(1539..2189)
                     /locus_tag="VFU72_04115"
     CDS             complement(1539..2189)
                     /locus_tag="VFU72_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5422703.1"
                     /translation="MRRALFLDRDGTLVEPRHYPSRPEELVLYAGIGPELRRIQQAGF
                     ALIVVTNQSGIARGYFDEADLRAMHDHLTCELARDGVRLDGIYHCPHHPDGVIPALAI
                     GCDCRKPRPGLLLRAGAEHALDLTRSWFVGDILDDIEAGNRAGCRSLLVDLGTEPPPE
                     DALRRPGFVARDTRHALRLIRFLEGLGPAVALDYRPARWRREPAVAALGGCDGRRD"
     gene            complement(2189..>2825)
                     /locus_tag="VFU72_04120"
     CDS             complement(2189..>2825)
                     /locus_tag="VFU72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5422704.1"
                     /translation="SPSLLDTGRFLARDRYLAAVDGVLGERRALLDRAIGALQQRSTV
                     IAEAASCIVDALRGGHKVLVVGNGGSAAEAQHFACELMGRFQQERAPYAVLALTADST
                     ILTALGNDYGYRQVFARQVAGLGQPGDLLLAFSTSGESPNVVEAALAARGRGMTVVAL
                     TGERASQLEKLADLTLRAPCALTPVAQEIHMLLTHVLCGVVEAELAAGEVV"
BASE COUNT          390 a         1033 c         1041 g          361 t
ORIGIN      
        1 tccagccgta cggccagcag gttgcgcgcg cgctcccagg ccgggtcgat catgctcacc
       61 tctcgcctcc aagcccgtcg cccgccagcg ccacgatacg gtcgatcacg cccgaggtgc
      121 tcaggtcgcc gaccagcggc aggatcacca cacgcccgcc gacctcggcc accgcctcgg
      181 cctccggcaa gtcctcgccc gcgtagtcgc cccccttgac gtggatgtgc ggccgcaggt
      241 cgcggatcag cgcttcgggg gtctcttcgt cgaagaggag agcgtggtcg accgcgtcca
      301 gcgcggccac cagcgccagg cggtgctcct cgctgttgat cgggcggttc ttgcctttga
      361 gcagccgcgc gctgcggtcg gtattgacgc cgaccaccag cacgtcgccg agcgcgcgcg
      421 cccggcgcag gaattcgacg tgcccggcgt gcaggatgtc gaagacgccg ttggtgaaga
      481 cgatcttgcg gccgcgctcg cgcgccgcgt cgagctgggc cgccaccgcg ccgaacacgt
      541 cgaggtcgcc ggcgcgcggc aggtgctcgc gcaggctgat ccgctgcagc agctcctgat
      601 agagcacgac cgccgtgcgc cgcttgctga ccgcgatgcc ggccgcttcg atcccgatct
      661 ggatcgcctc ggtgcagcgc gcgcccgacg ccagcgccag cgccagcgcg gccaggaacg
      721 agtcgccggc ccccacatcg ctgggattgg ccaccgggtg ggccgggaaa tgccgcgccc
      781 gcccgcgccg gtcgagcagg cagacgcccc ccgcgccgag cgtcaccgcg acgtgctcgg
      841 tctcgatcgc ctcgaccagc cgccgcccgg cgcgcttgac ctcgtgcagg ttcagccctt
      901 cgccggccgg caccgcgccg tcgacggcca gcagcgcctc caggtgattg ggagtgatga
      961 tggtcgcgtc ggactcgcgg aagcggcgca ggtgcttgga gtcgaccgcc agcacacagg
     1021 ggcgcgcctg ccgcagcgcg cgcaggtggc gcaccagtcc gtcgctgagc acgccgtagt
     1081 tgtagtcgga gatcacgacc aggtcgcagc gcgggaaggc gtggtcgagc gtggccagca
     1141 ggcgctcgta ggtacggtgg ccgagcgatt cggcgccgcc gtcatcgaag cgcaccacgt
     1201 actggccgtt ggccaggatg cgcagcttgt gcagcgtgct gatcgcgtca tcctcgacca
     1261 gccaggagtc gtcgacgtcg tggtcgcgca gcgcggcgcg gagcagcgcc cccgctggat
     1321 cggcgccgac cacccccagg aagaggacct cggcccccag cgcggccagg ttggcggcgg
     1381 tgttggccgc cccgcccggc agccgctcct cggccgtctt gcgcaccacc ggcaccggcc
     1441 cctcggcgca gagccgcgcg gcggtgccct ccaggtagct gtcgagcatc gcgtcgccga
     1501 tcaccagcgc gcgcaggcgg cgaaagccgc gcacggtctc aatcgcgccg gccatcgcat
     1561 cctcccaacg ccgccaccgc cggctcccgc ctccagcggg ccgggcggta gtcgagcgcg
     1621 accgccgggc cgagcccctc caggaagcgg atcagccgca gcgcgtggcg tgtgtcgcgg
     1681 gcgacgaagc ccgggcggcg cagcgcatcc tcgggcggcg gctcggtgcc gagatcgacc
     1741 agcagcgagc ggcagccggc gcggttgccc gcctcgatgt catccaggat gtcgcccacg
     1801 aaccaggagc gcgtcaggtc gagggcgtgc tcggctccgg cgcgcagcag caggccgggg
     1861 cgcggcttgc ggcagtcgca gccgatcgcc agggcgggga tgacgccgtc ggggtggtgc
     1921 gggcagtggt agatgccgtc gagccgcacg ccgtcgcgcg ccagctcaca cgtcaggtgg
     1981 tcgtgcatgg cgcgcagatc agcctcgtcg aagtagccgc gggcgatgcc cgactggttg
     2041 gtgaccacga tcagggcgaa gccggcctgc tggatgcggc gcagttcggg gccgatgccg
     2101 gcgtagagga cgagctcctc cggccgggag gggtagtggc gcggctcgac cagcgtgccg
     2161 tcgcggtcca ggaagagggc gcggcgcatc agacgacctc cccggcggcc agctccgcct
     2221 cgaccacgcc gcagaggacg tgcgtcagca acatgtggat ctcctgcgcg acgggcgtca
     2281 gggcgcaggg ggcgcgcagc gtcaggtcgg ccagcttctc gagctggctg gcccgctccc
     2341 cggtcagcgc taccaccgtc atgccgcggc cgcgcgcggc cagcgccgcc tcgaccacgt
     2401 tgggcgactc gccgctggtg ctgaaggcca gcagcagatc gcccggctgg cccaggcccg
     2461 ccacctggcg cgcgaagacc tggcggtagc cgtagtcgtt gcccagcgcg gtcagaatgg
     2521 tgctgtcggc ggtgagcgcc aggacggcgt agggcgcgcg ctcctgctgg aagcggccca
     2581 tgagctcgca ggcgaagtgc tgcgcctcgg cggcgctgcc gccattaccg acgaccagca
     2641 ccttgtggcc gccgcgcaga gcgtcgacga tgcatgaggc ggcctcggcg atcacggtgg
     2701 agcgctgctg caaggcgccg atagcgcggt cgagcagggc gcggcgctcg cccagcaccc
     2761 cgtcgaccgc ggccagatag cgatcgcggg ccaggaagcg gccggtgtcg agcaaggatg
     2821 ggctg
//
LOCUS       DASUBI010000213         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_289718, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000213 DASUBI010000000
VERSION     DASUBI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289718"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..379)
                     /locus_tag="VFU72_04125"
     CDS             complement(161..379)
                     /locus_tag="VFU72_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5422705.1"
                     /translation="MKYAAFIRYGNSDQIATVRPRHREYLGSLKEQGKLAASGPFTDD
                     SGALIQADPFRQAGVFQEIELKEWRQVF"
     gene            516..881
                     /locus_tag="VFU72_04130"
     CDS             516..881
                     /locus_tag="VFU72_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEU5422706.1"
                     /translation="MDDLHPSARGQDHLLQAIQVCTVGHRVCLQSLNHALAAGGRMAA
                     PEFVTRLLDCAALCQAAVDFMLRESPLYPRVCALCAEAADLCAHDAALFDDDPVMHAC
                     AETCARAAAACRQTARLPL"
     gene            916..1827
                     /locus_tag="VFU72_04135"
     CDS             916..1827
                     /locus_tag="VFU72_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU5422707.1"
                     /translation="MARVVITQDRQEIGRAIDEALGRLDLDALVRGKMVAVKPNETWA
                     SPEDISGVTQADSLRATLRRLKEHNPRELIVSGGAGAAQTADVFRYAGLMDVIHDEGA
                     EFFDHNQPPFVSVDLHYQPEHDFAGPQQAVMVNPRVLEYETLISLAQLKLHETATVTL
                     SLKNIAMSFPAADYYGHPRATQEHENSFFADMHSFIVAMAQRFPIDLAIIVGHPAMIA
                     SGPLGGKAVETGLVIAGTDPVAADVVGARLLGFRPQGVRYLWEAGKIELGETKVDRIE
                     FPAMSLEDAIGAFTEAAYGERLTFKHA"
     gene            complement(2004..2675)
                     /locus_tag="VFU72_04140"
     CDS             complement(2004..2675)
                     /locus_tag="VFU72_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422708.1"
                     /translation="MPKILVVVAEAILRDRLTAELDAAGHDARGAASGADALLAAAEP
                     LDLVILDALLPGAEGFALCRRLREMGVPAILLIAELDGVTEQLAREAGADDLLLAPFE
                     MGELLDRVRALLPPQPVSAPIEEPVVVDDLVIDLAANRVLRGAVPVALKPKEMELLAF
                     LVRHRGAVCSTRQLLGEVWGYEYFGDSRTLAVHIHGLREKLGDDPSEPHLIQTVRGAG
                     YRFMG"
     gene            2969..>3193
                     /locus_tag="VFU72_04145"
     CDS             2969..>3193
                     /locus_tag="VFU72_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422709.1"
                     /translation="MGVRHTDSSTPADAGKSSQTGQFSRYRRLLTRYLRPEWPRVLLL
                     ALIIGATIGVQVAAPLVGSRFVDHATAGAPL"
BASE COUNT          496 a         1132 c         1078 g          487 t
ORIGIN      
        1 ggctgccgcc tgcgcggccg cgacaatact cgccggtggc cggcgcaccc atcatgtgac
       61 gttctacctt accgaggcag ccgaaggatc tctcatcaat tgggggcgac aggaatcgag
      121 gtggaatggg cacaagccct ctccacgagg ggcgggcgcg ctagaagacc tggcgccact
      181 ccttcagctc gatctcctgg aagacgccgg cttggcggaa ggggtcggcc tggatcagcg
      241 cgccgctgtc gtcggtgaac ggtccggaag cagccagctt gccctgctct ttgaggctgc
      301 cgaggtattc gcggtgcctg ggccgcaccg tggcgatctg gtcgctgttg ccgtagcgga
      361 tgaacgcggc gtacttcatg tcccctccca ggtcagcgtc cgattccgac ggcgcgatcg
      421 tagcgcagcc gcgctggatc cgtcaggttt gccaggggca cgtcctttgc gccgtggcgc
      481 caggtgtcga ggtgccgcga aaggagccgg tgacgatgga cgaccttcac cccagcgcac
      541 gcggccagga tcatcttctc caggcgatcc aggtctgcac cgtcggccac cgcgtctgcc
      601 tgcagagttt gaaccacgcg ctggccgcgg gcggtcgcat ggccgctccc gagttcgtca
      661 ctcgcttgct cgactgcgcg gcgctgtgcc aggcggcggt cgatttcatg ctgcgcgagt
      721 cgccgctcta cccgcgcgtc tgcgcgctct gcgccgaggc cgccgacctc tgcgcccacg
      781 acgcggctct cttcgacgac gatccggtca tgcacgcctg cgccgagacc tgcgcgcgtg
      841 ccgccgcggc ttgccgtcag acggcgcggc tgccgctata atcccccggt tcaggccaac
      901 ggggaggagc gagcgatggc gcgagtggtg atcacgcagg accggcagga gatcggccgg
      961 gcgatcgacg aggcactcgg ccggctcgat ctcgacgcgc tggtgcgcgg caagatggtg
     1021 gcggtcaagc cgaacgagac ctgggcctcg cccgaggaca tcagcggcgt gacccaggcc
     1081 gacagcctgc gggccacgct gcgccggctc aaagagcaca atcccaggga gttgatcgtc
     1141 agtggcggcg cgggcgcggc ccagaccgcc gacgtcttcc gctacgccgg gctgatggat
     1201 gtcatccacg acgagggcgc cgagttcttc gaccacaatc agccgccctt cgtttcggtc
     1261 gacctccact accagcccga gcacgacttc gccgggccgc agcaggcggt gatggtcaac
     1321 ccgcgcgtgc tggagtacga gaccctgatc tcactggccc agctcaagct gcacgagacc
     1381 gccaccgtca ccctgtcgct gaagaacatc gccatgtcct tcccggcggc ggactactac
     1441 ggccacccgc gcgccaccca ggagcacgag aacagctttt tcgcggacat gcactccttc
     1501 atcgtggcca tggcccagcg cttcccgatc gacctggcca tcatcgtcgg gcatccggcc
     1561 atgatcgcca gcggcccgct cggcggcaag gcggtcgaga ccggcctggt gatcgccggc
     1621 accgatccgg tggcggcgga cgtggtcggc gcgcggctgc tcggcttccg cccgcagggc
     1681 gtgcgttatc tgtgggaagc gggcaagatc gagctgggtg agaccaaggt ggaccggatc
     1741 gagttcccgg ccatgtcgct ggaggacgcc atcggcgcct tcaccgaagc cgcctacggc
     1801 gagcgcctga ccttcaagca cgcgtgaccg gccctctacc cgtttggtag gggcagaccc
     1861 ctgtgtctgc cctcgttgcc caacgcatcg gcaccgggga tatgggaaac cttgggcggc
     1921 gcacaacgga ccaagtgcca ggccgcgagg gcaggccccc gtgctgcccc tacagggggt
     1981 agcaccgcgc ctcttggcgg ctgtcacccc atgaagcggt agccggcgcc gcgcacagtc
     2041 tggatcaggt gcggctcgct gggatcgtcg cccagcttct cgcgcaggcc gtggatgtgg
     2101 accgccagcg tacggctatc gccgaagtac tcgtagcccc agacttcgcc cagcagttgg
     2161 cgcgtggagc agaccgcgcc ccggtgccgc accagaaagg ccagcagctc catctccttc
     2221 ggcttgagcg ccaccggcac ggcgccgcgc agcacccggt tggcggccag gtcgatcacc
     2281 agatcgtcga ccaccaccgg ttcctcgatc ggcgcgctca ccggctgggg aggtaagagc
     2341 gcgcggacgc ggtccagcag ctcgcccatc tcgaaggggg ccagcaggag atcgtcggcg
     2401 ccagcctcgc gggccagctg ctcggtcaca ccgtccagct cggcgatgag gaggatggcg
     2461 ggcacgccca tctcgcgcag ccgccggcag agcgcgaagc cctctgcccc cggcaggagc
     2521 gcgtcgagga tcaccagatc gagcggctcg gcggcggcca gcagcgcgtc ggccccgctg
     2581 gcggctccac gggcatcgtg tccggcggcg tccagctcgg ccgtcagccg gtcgcggagg
     2641 atggcttctg ctacgacaac gaggatcttg ggcacggctc accccctgtc gcctcgacac
     2701 ccacgaatgg tagcaggcag gcgccgctcg tgcgtgacaa cccgcccgtg accagtgaca
     2761 ggggtctcgc gcctgggccg gtgggccggc tggcccttgc cgcggcgcgt ccgcctccgc
     2821 tggcgcaacc ctccacccgg ctgctagaat ccgatccggc cgggcaatct gctccggtac
     2881 tcgacgccag gcagccacga ggagtgtccc atgcagccaa cgttgaagat cccagcagcc
     2941 gagcggttct tcaacgagag agtgatgcat gggagttcgc cataccgatt cctcgacacc
     3001 ggcagacgcc ggtaagtcca gccaaacagg acaattcagc cgctaccgcc ggctgctgac
     3061 gcgctatctc cggccggagt ggccacgggt gctgctgctg gcgctgatca tcggcgccac
     3121 catcggcgtg caggtcgccg cgccgctggt cggtagccgc ttcgtcgatc acgcgaccgc
     3181 cggcgcgccg ctg
//
LOCUS       DASUBI010000214         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1266106, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000214 DASUBI010000000
VERSION     DASUBI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266106"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFU72_04150"
     CDS             complement(<1..388)
                     /locus_tag="VFU72_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5422710.1"
                     /translation="MTEVEVPAGARTRGGTAPRRLLRRLRGRRFAVLAILGPGLIAAT
                     AGDDAGGIATYASVGAEFGFALLWALVLITISLALVQEMAERMGAVTGKGFAELVRER
                     MGIRVTAFVMLALLVANTGLVISEFAG"
     gene            complement(385..1662)
                     /locus_tag="VFU72_04155"
     CDS             complement(385..1662)
                     /locus_tag="VFU72_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5422711.1"
                     /translation="MGARRAAANPNVLYLSQILGKHILDVQGEPIGRIQDVVVQFGSG
                     PHPPVKGLVARQGQRDFYLDWSQVAELAPRGARLATPKVDLRAFERRPGEVLLRKDVL
                     DKQLIDVNGRRLVRANDLELARVYGRYRLVGVDVGFQAIARRLGPRALAARFRGSRLI
                     DWANVESFTTDVPTVRLRVPHARVARLHPVEIAQIMEDLAPRQSQEVLASLDDETAAE
                     AMQEMDPDDAADLLERLNQERAADILEEMEPDDAADVLAEMDEERAAALLHLMEPDEA
                     ADVRELLTYEEDSAAGMMTTEFVVLPRHLTAADTLRRLRELEEPPEQLYDLYVIDAFD
                     NQRLAGVVPLRDLILAPPEATLSALAQPAHPVSPGISAREAAAIMAEYNLVMLPVVDE
                     DEHLLGVVSVDDAMELLLPEPHEWLAPSRIFRQ"
BASE COUNT          306 a          725 c          713 g          285 t
ORIGIN      
        1 tgccggcgaa ctccgagatc accagaccgg tgttggccac cagtagcgcc aacatcacga
       61 aggccgtgac acggatgccc atgcgctcgc gcaccagctc ggcgaagccc ttgccggtga
      121 cggctcccat gcgctcggcc atctcctgaa ccagcgcaag gctgatggtg atcagcacca
      181 gcgcccagag cagcgcgaag ccgaactcgg cgccgaccga ggcgtaggtg gcgatgccgc
      241 cggcatcgtc gccagccgtg gcggcgatga ggccggggcc gaggatcgcc aggacggcga
      301 agcgtcggcc acgcagccgg cgcagcaggc gacgcggcgc cgtgccgccg cgcgtccgcg
      361 cccctgccgg cacctcgacc tccgtcactg acgaaagatc cgcgagggag ccagccactc
      421 gtgcggctcc ggcagcagca gctccatcgc gtcgtcgacc gagacgacgc ccaggagatg
      481 ctcgtcctcg tcgaccaccg ggagcatgac caggttgtac tcggccatga tcgcggcggc
      541 ctcgcgcgcg gagatacccg gcgagaccgg gtgggccggc tgggccagcg ccgagagcgt
      601 cgcctcgggt ggcgccagga tcagatcgcg cagcgggacc acgccggcca ggcgctggtt
      661 gtcgaaggcg tcgatgacgt agagatcgta gagctgttcg ggcggctcct ccaactcgcg
      721 caggcgccgc agcgtgtcgg cggcggtgag atgtcgcggc aggacgacga actccgtcgt
      781 catcatgccg gcggcgctgt cctcctcata ggtcagcagc tcgcgcacgt cggcggcctc
      841 gtccggctcc atcaagtgga gcagcgcggc cgcgcgctcc tcgtccatct ccgccagcac
      901 atcggcggcg tcgtccggct ccatctcctc cagaatgtcg gcggcgcgct cctggttgag
      961 gcgctccagc aggtcggcgg cgtcgtcggg gtccatctcc tgcatcgcct cggcggcggt
     1021 ctcgtcgtcg agcgaggcga gcacctcctg gctctggcgc ggcgccagat cctccatgat
     1081 ctgcgcgatc tcgaccgggt gcagccgggc cacgcgcgcg tggggcacgc gcaggcgcac
     1141 cgtcggcacg tcggtggtaa aggactcgac gttggcccag tcgatcaggc ggctgccgcg
     1201 gaagcgggcg gccagcgcgc gcggccccaa acggcgcgcg atcgcctgga agccgacatc
     1261 cacgccgacc aggcgatagc gcccgtagac gcgcgccagc tccaggtcgt tggcgcgtac
     1321 cagtcgccgc ccgttgacgt cgatgagctg cttgtcgagc acgtccttgc gcagcagcac
     1381 ctcgccgggc cgccgctcga aggcgcgcag atccaccttc ggcgtcgcca gtcgcgcccc
     1441 cctcggcgcg agctcggcga cctgcgacca gtcgagatag aagtcgcgct ggccctggcg
     1501 cgccaccagc cccttgaccg gcgggtgggg gccgctgccg aactgcacca ccacatcctg
     1561 gatgcgaccg atgggctcgc cctgcacatc gaggatgtgc ttgccgagga tctgcgacag
     1621 gtagagcacg ttcggattcg cggcggctcg ccgagcgccc atgtccgagc cctttctgcg
     1681 tgcggcgttg cgagtgccgg ccgtcggggt acgtgccggg gcatggccgc tccgccaggg
     1741 tctcgaccgg caaagtcagg tccaggtgca gccccgagcg ctccttcgcg ccgacgcgct
     1801 cgaggtgctc gggctcagcg agcgagcggt tgatcgagtg gggagatggt ggaaacctcg
     1861 agcgcgttga gtgagggagg attcccgtcg gtggtactca tacgctccgt cccaaccgaa
     1921 gaccacgcct ccccgctggg gaggacgacg cgctaggact atagcagccg ccgccgcccg
     1981 tcgcaattcg gcggcggagc gctcaatccg ccggccatta ccacgtggc
//
LOCUS       DASUBI010000215         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1022613, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000215 DASUBI010000000
VERSION     DASUBI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022613"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFU72_04160"
     CDS             complement(<1..588)
                     /locus_tag="VFU72_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422712.1"
                     /translation="MPRIRLGVALWSQCASWDDYQAAAQLVDRLGYDTLLTNDHLLSE
                     VGPPDQPKFEAWTALAAWATVTSHAMLGHWVGSNTFRHPALVAKMAATVDHASHGRLL
                     LGIGAGWFAHEHEVFGLDYGRSPGERLAWLDEAVSILRPLFDGETVTHDGPRYQVRDL
                     TLYPPPVRGSIPIMIGGAGERKTLRTVAKYADLWDA"
     gene            757..1029
                     /locus_tag="VFU72_04165"
     CDS             757..1029
                     /locus_tag="VFU72_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5422713.1"
                     /translation="MKDISATIISKNQITIPAEVRRHLGLKQGDKVAFVLGDGGTVEL
                     RALKYPSVASRAGAAGRLTKSLPLDFDEMLEIAREDALEHDFPPAR"
     gene            1038..1535
                     /locus_tag="VFU72_04170"
     CDS             1038..1535
                     /locus_tag="VFU72_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422714.1"
                     /translation="MVYTADNDHLAGITLLEPQRLKGRAQNERAQRCILKLAPRRENR
                     RGVIRAEARFALNNSPDRVESGALMSRRFRPIDVADFPELRRIADEVQQTGEPRLLQR
                     DGEDLAVIVPIAEARRSARRPKTEADHQAFLSAAGGWADLDTDKLVADIYADRRRSNR
                     PPIEL"
     gene            1624..1923
                     /locus_tag="VFU72_04175"
     CDS             1624..1923
                     /locus_tag="VFU72_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5422715.1"
                     /translation="MAISIITFAEIYEGIYYGHDPARHEQFFRRFLQGVSVLDLTRTV
                     ARRYARLRGELRQLGQMIDQPDLFIAATAIEYDLELLTRNVRDFERIPGLILARP"
     gene            2118..3101
                     /gene="ftsY"
                     /locus_tag="VFU72_04180"
     CDS             2118..3101
                     /gene="ftsY"
                     /locus_tag="VFU72_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5422716.1"
                     /translation="MVFGRLFRRKTEPEPKIEAGLKKTRRGLFQDISRLFERSEITED
                     LYEDLEALLIQADLGVETTVELLENLRARIERERIRQPAEAREALRTEMIALLEHATR
                     NRRIKIYQRGVPFAILVVGVNGTGKTTTIAKLAKLHQDAGRTPVLVAGDTFRAAAIDQ
                     LKVWGERLGVTVIAHDPGADPGAVVFDGMQAAYNRKADILLIDTAGRLHTKFNLMEEL
                     KKIKKVIQRHVAEAPQEVLLVIDATTGQNGLVQAKKFAEAAEITDIAIAKLDGTARGG
                     IAFAISRELGIPISYVGTGEKVTDLAEFNPETYVDALFFGDEDLEQGEGGA"
     gene            3103..4623
                     /gene="ffh"
                     /locus_tag="VFU72_04185"
     CDS             3103..4623
                     /gene="ffh"
                     /locus_tag="VFU72_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5422717.1"
                     /translation="MFETLSDRLNDVFQKIGKKGRLTEADVEQATKEVRLALLEADVN
                     YKVVSNFVKAVRERAVGEEVLKSLTPAQTVISIVNEELIRILGEERVPLESATPPPTI
                     VMLVGLQGSGKTTHVAKLAAHLKREGKRPLLVAADVYRPAAIQQLETLGRQVNVPVFA
                     EGTQANPVEIARHGIELARREGYNPVIIDTAGRLQIDERMMAELEEITAAVQPTETLL
                     VADAMTGQEAVNVAQEFHRRLDLTGMVLTKMDGDARGGAALSIRSVVGVPIKFIGTGE
                     KIDALEPFYPDRLAGRILGMGDVLSLIERAQEQISEEESLELQEKVLEGAFDLEDFLH
                     QLQQIKRMGPLTQLLEMIPGVGQAIRQQDVQISDDQFKHIEAIIYSMTAAERRNPEII
                     RPSRRRRIAAGSGTSQADVNQLLTQFKQMQKMMQQLGGLGTGGKRGKLRGLLKGNPFG
                     GMNPAELEAMMQGGEGAFPAGRPLPAPPRGTQLPRKHSSSGGGSKKAKKKAKGRRR"
     gene            4704..5783
                     /locus_tag="VFU72_04190"
     CDS             4704..5783
                     /locus_tag="VFU72_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422718.1"
                     /translation="MEYVSLDAPTGSRHERLLGLPLFYKVLVGNSIIVVAGAIIGTAA
                     TLKVSAETSTAFDLVAIFAIIGTFASILVNWIVLRAALRPLSVLEEAVDDVRHGNFGV
                     RAEKVAFSDPSMDALIDTFNGMLDAVERYRIQLGDLSMQVLSAQEEERKRISRELHDN
                     TAQTLTAQLLRLKTIEARGGTLDPAGLTRLIETTAQALEEVRYMAHELRPPSLDDLGL
                     RASLEGLAAQYDERFSLPVQVRGDRLRRLGPDAELAIYRIVQEALTNVAKHAGASGAR
                     VELVCDQATLRVRISDDGRGFDEREVALREGAGLGLFGMQERAALVGGTLAISSRSGG
                     GTVVALAIPLGGARAHPDSRLEVLA"
     gene            5780..6457
                     /locus_tag="VFU72_04195"
     CDS             5780..6457
                     /locus_tag="VFU72_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422719.1"
                     /translation="MTTTISSRVISVLLADDHPIVRAGLRALLEAQPDISVVGEAGSG
                     EDSVELCQRHRPDVAIMDISMPGGGLEAMRKIAGLGLSTRVLILTVHAEERYLLPVLE
                     AGGSGYVRKSSLHTDLIDAIRMVARGEVFLDPAATRMLLKGYLDRADDGRELDPGKIL
                     SEREREVLKLTAEGYTAKEIGDRLYLSPKTVETYRHRIVQKLGFSHRSELVRYALRAG
                     LLDAGAE"
     gene            complement(6931..>7112)
                     /locus_tag="VFU72_04200"
     CDS             complement(6931..>7112)
                     /locus_tag="VFU72_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422720.1"
                     /translation="WLAWFGLAAAVILLAAIMFMPFMILPLWTLGAAVALLRRETAPR
                     TASRQQPAVPQAGQP"
BASE COUNT         1213 a         2430 c         2415 g         1054 t
ORIGIN      
        1 ggcgtcccac agatcggcgt acttcgccac cgtgcgcagc gtcttgcgct cgcccgcgcc
       61 gccgatcatg atcgggatgc tgccgcgcac cggcggcggg tagagcgtca agtcgcgcac
      121 ctggtagcgc gggccgtcgt gcgtcaccgt ctcgccgtcg aagagagggc gcaggatgct
      181 caccgcctcg tcgagccagg cgagccgctc gccggggctg cggccgtaat cgaggccgaa
      241 gacctcgtgc tcatgggcga accatccggc gccgatgccg agcaggagcc ggccgtgcga
      301 ggcgtggtcg acggtggcgg ccatcttggc caccagcgcc gggtggcgga aagtgttgct
      361 gccgacccag tggccgagca tggcgtgact ggtcaccgtg gcccaggcgg ccagcgcggt
      421 ccaggcctcg aacttgggct gatcgggcgg gccgacctcg ctcagcaggt ggtcgttggt
      481 cagcagcgtg tcgtagccca ggcgatcgac caactgcgcc gccgcctgat agtcgtccca
      541 gctcgcgcac tgcgaccaga gcgccacacc cagccgtatc cgcggcatcc gccccgcccc
      601 cttcccgata cgactgcgct ccccaatgct gtacagtgta cgggattttg ggcgctcacg
      661 atatactgag agcgtgtttc gtccacagca ctggtgccgt gtcggagcgt tccgcgtcga
      721 tgtgcctgct gtcgtctccc tgccgctgga gcctcaatga aagatatctc cgccaccatc
      781 atcagcaaga accagatcac gatcccggcg gaggtccgcc gccacctcgg tctcaagcag
      841 ggcgacaagg tcgccttcgt cctgggcgat gggggtacgg tggagctgag ggctctgaaa
      901 tatccctcgg tcgcctcgcg agccggcgcc gctggtcgtt tgaccaaatc gctaccgctc
      961 gacttcgacg agatgctcga gatcgcccgg gaagacgcgc tggagcatga tttcccgccc
     1021 gcgcgatgag ggcgcccttg gtctataccg cggacaatga ccacttggcg ggcatcaccc
     1081 tcctggagcc gcagcggctc aaaggtcgcg ctcagaacga acgtgcccag cggtgtatac
     1141 ttaaactcgc cccgcgccgt gagaaccggc gcggcgtcat tcgagccgag gcacgtttcg
     1201 ccctaaacaa ctccccagat cgagttgaga gcggggcgct catgagccgc cgattcagac
     1261 cgattgatgt cgcggacttc ccggagcttc ggcgcatcgc cgacgaggtg cagcagacgg
     1321 gcgagccgcg cctgctgcaa cgcgacggcg aggacctggc ggtcatcgtg ccaattgccg
     1381 aggcacgcag gtcagcacgc cgccccaaga ctgaggccga ccaccaggcg ttcctctccg
     1441 cggctggtgg ctgggccgac ctcgataccg acaagctcgt cgccgacatc tatgccgacc
     1501 gccgtcgtag taaccggccg ccgattgagc tatgaggtac ctgatcgata gcgattgggt
     1561 cgccgattat ctcaaaggcc gggagtctgc ggcaatgctg ctggctgatc tttccccgcc
     1621 ggtttggcca tcagcatcat caccttcgct gagatttacg agggcatcta ctacgggcac
     1681 gatccagcgc gtcacgagca atttttccgg cgcttcctgc aaggcgtctc ggttctcgat
     1741 ttgacacgca cggttgctcg ccgctacgcg cggctccgag gggaactgcg ccaacttggg
     1801 caaatgatcg accaacctga tcttttcatc gcggcgacgg cgatcgagta cgacctcgag
     1861 ctgttgacac gtaacgtgcg cgacttcgag cgaatccccg gtctcatcct ggctcgtccg
     1921 tagcgccttc tctgccgggg cgaacggtgg atatagggta gcatcgaggc gcctgacccc
     1981 gcgcccggag cgaccgcacc caacggtgta tacttgactt gcgccgcgcc gtgagaaccg
     2041 gcgcggcgtc gtgctacacc gcgcgcactt caacgtcaac catgatcaat atgggcacac
     2101 ccgaagagga ttgagcggtg gtattcggca ggctctttcg ccgcaagaca gaaccagaac
     2161 cgaagatcga ggcgggcctc aagaagacgc gccgcggttt gtttcaggac atctcccggc
     2221 tcttcgagcg cagcgagatc accgaggacc tctacgaaga ccttgaagcg ctcttgattc
     2281 aggccgacct cggcgtcgag acgaccgtcg agctgctgga gaacctgcgc gcgcgcatcg
     2341 agcgcgagcg catccgccag ccggccgagg cgcgcgaggc gctgcgtacc gagatgatcg
     2401 ccctcttgga gcacgccacc cgcaaccggc gcatcaagat ctatcagcgc ggcgtgccct
     2461 tcgcgatcct ggtcgtcggc gtcaacggca ccggcaagac taccaccatc gccaagctgg
     2521 cgaagctcca ccaggacgcg ggccgcaccc cggtgctggt ggccggcgac accttccgcg
     2581 ccgccgccat cgatcagctc aaggtctggg gcgagcggct cggcgtcacc gtcatcgcgc
     2641 acgatccggg cgccgacccc ggcgccgtgg tcttcgacgg catgcaggcc gcctacaacc
     2701 gcaaggccga catcctgctg atcgacaccg ccggccggct ccacaccaag ttcaacctga
     2761 tggaggagct gaagaagatc aagaaggtca tccagcggca cgtggccgag gcgccgcagg
     2821 aggtgctact ggtcatcgat gccaccaccg gtcagaacgg gctggtgcag gccaagaagt
     2881 tcgccgaggc ggccgagatc accgatatcg ccatcgccaa gctcgacggc acggcgcgcg
     2941 gcggcatcgc cttcgccatc tcgcgcgagc tgggcatccc gatcagctac gtcggcaccg
     3001 gcgagaaggt caccgacctg gccgaattca accccgagac ctacgtcgac gcgctcttct
     3061 tcggggacga ggatctagag cagggtgagg gaggggcctg agatgtttga gacgttgagc
     3121 gaccggctca acgacgtctt ccagaagatc ggcaagaagg gccggctgac cgaggccgac
     3181 gtcgagcagg cgaccaaaga ggtgcgcctg gcgctgctgg aggccgacgt caactacaag
     3241 gtggtcagca acttcgtcaa ggcggtgcgc gagcgcgccg tcggcgagga ggtgctcaag
     3301 agcctgaccc cggcccagac ggtcatctcg atcgtcaacg aggagctgat ccgcatcctc
     3361 ggcgaggagc gcgtgccgct ggagagcgcc acgccgccgc cgacgatcgt gatgctggtc
     3421 ggcctgcagg gctcgggcaa gacgacccac gtggccaagc tggcggcgca cctcaagcgc
     3481 gagggcaagc gcccgctctt ggtggccgcc gacgtctacc gcccggccgc catccagcaa
     3541 ttggagacgc tggggcgaca ggtcaacgtg ccggtcttcg ccgagggcac gcaggccaac
     3601 ccggtcgaga tcgcgcgcca cggcatcgag ctggcgcggc gcgagggcta caacccggtc
     3661 atcatcgaca ccgccggccg gctgcagatc gacgagcgca tgatggccga gctggaggag
     3721 atcaccgccg ccgtgcagcc gaccgagacg ctgctggtgg ccgacgctat gaccggccag
     3781 gaagcggtca acgtggcgca ggagttccac cgccgcctgg acctgaccgg catggtgctg
     3841 accaagatgg acggcgacgc ccgcggcggt gcggcgctct cgatccgctc ggtggtcggc
     3901 gtgccgatca agttcatcgg caccggcgag aagatcgacg cgctggagcc cttctacccc
     3961 gatcgcctgg ccgggcgcat cctcggcatg ggcgacgtgc tcagcctgat cgagcgcgcc
     4021 caggagcaga tcagcgagga ggagagcctc gagctgcagg agaaggtgct ggagggcgcc
     4081 ttcgacctgg aggacttcct gcaccagctg cagcagatca aacgcatggg cccgctgacc
     4141 cagctgctgg agatgatccc cggcgtcggg caggcgattc gccagcagga cgtgcagatc
     4201 agcgacgatc agttcaagca catcgaggcg atcatctact cgatgacggc cgccgagcgc
     4261 cgcaacccgg agatcatccg gcccagccgg cgccgccgca tcgcggccgg cagcggcacc
     4321 agccaggccg acgtgaacca gctcttgacg cagttcaagc agatgcagaa gatgatgcag
     4381 cagcttggcg gcctgggcac aggcggcaaa cggggcaagc tgcgcgggtt gctgaagggc
     4441 aaccccttcg gcggcatgaa ccccgccgag ctagaggcca tgatgcaggg tggcgagggc
     4501 gccttcccgg ctggccggcc gctgccggcg ccgcctcgcg gaacccagct cccccgcaag
     4561 cacagcagca gcgggggtgg cagcaagaag gccaagaaga aggccaaggg ccgccgccgc
     4621 taggtgaccg ccaggcgacg gccttccccg cgggctcgcc gcggtcccca agatgggcat
     4681 cggcatggac acggagccag gcgatggagt acgtgagtct cgacgcgcca accggttccc
     4741 ggcacgagcg gctgctcggc ctgccgctct tctacaaggt gctggttggc aactcgatca
     4801 tcgtggtggc cggcgcgatc atcggcacgg cggccacgct caaggtctcg gccgagacca
     4861 gcacggcctt cgatctggtc gccatcttcg ccatcatcgg caccttcgcc agcatcctcg
     4921 tcaactggat cgtgctgcgc gccgcgctgc ggcccctctc ggtgctggag gaggcggtcg
     4981 acgacgtgcg ccacggcaac ttcggcgtgc gggccgagaa ggtcgccttc agcgacccca
     5041 gcatggacgc gctgatcgac accttcaacg gtatgctcga cgccgtcgag cgctaccgca
     5101 tccaactggg cgacctctcg atgcaggtcc tctcggcgca ggaggaggag cgcaagcgca
     5161 tctcccgcga gctccacgac aacacggcgc agacgctgac cgcgcagctg ctgcggctga
     5221 agacgatcga ggcgcgcggc ggcacgctcg acccggcggg gctgacccgc ctgatcgaga
     5281 ccacggcgca ggcgctggag gaggtgcgct atatggcgca cgagctgcgc ccgcccagcc
     5341 tggacgatct cggcctgcgc gcctcgctgg agggcctggc tgcgcaatac gacgagcgct
     5401 tcagcctgcc ggtccaggtc cgcggcgacc gcctgcggcg gctggggccc gacgccgagc
     5461 tggccatcta ccgcatcgtg caggaggcgt tgaccaacgt ggccaagcac gcgggggcga
     5521 gcggcgcgcg cgtggagctg gtctgcgacc aggccacgct acgggtgcgg atcagcgacg
     5581 acggccgcgg cttcgacgag cgggaggtcg cgctgcgcga gggggcggga ttggggttgt
     5641 tcggcatgca ggagcgagcg gcgctggtcg gcgggacgct ggccatctca tcgcgctcgg
     5701 gcggggggac agtggtcgct ctggcgatcc cgctcggcgg cgcccgcgcc caccctgatt
     5761 cgcgcttgga ggttctcgcg tgacgaccac catcagctct cgcgtcatca gcgtgctgct
     5821 ggccgacgac cacccgatcg tgcgcgccgg cctgcgggcc ctgctggagg cccaacccga
     5881 catttcggtg gtgggcgagg cgggcagcgg tgaggactcg gtcgagctgt gccagcgtca
     5941 ccggccggat gtcgccatca tggacatctc gatgccgggc ggcgggctgg aggcgatgcg
     6001 caagatcgcc ggcctgggcc tctctacccg tgtcctgatc ctgaccgtcc acgctgagga
     6061 gcgctacctg ctgccggtac tggaggccgg cggctccggg tacgtgcgca agtcgagcct
     6121 gcacaccgac ctgatcgacg cgatccgcat ggtggcgcgc ggcgaggtct tcctcgaccc
     6181 ggcggccacg cgcatgctgc tcaagggcta cctcgaccgc gccgacgacg ggcgcgagct
     6241 ggacccgggc aagatcctct ccgagcgcga gcgcgaggtg ctgaagctga cggccgaggg
     6301 ctacaccgcc aaggagatcg gcgaccgcct ctacctctca cccaagacgg tcgagaccta
     6361 ccgccaccgc atcgtgcaga agctcggctt cagccaccgc tcggaactgg tgcgctacgc
     6421 cctgcgcgcc ggcctgctcg acgccggggc ggagtaggcg ggcgcgcggg ttggttgcgg
     6481 cctcccgggg atgtccggtt ccgttcgtcg cgccgggcca aagccacagc gctaccaaga
     6541 cgaagcccac taaaggggct gagattccgc gctccacgcg attcgacgat gggacgcatc
     6601 agctctagct acagaaggga gatccttcgg ctgcggcctc cccacccctg tcatccttcg
     6661 ctgcgccgaa tgatctccca tcagcaccgt gcggcagtca acgcggcacg tggctgcgga
     6721 tcctcgccgc ttcgccgcgg ccggctcagg gacgtagggc cggaatggga gcgcgccgtg
     6781 cggttctctc tcagcgatgc cgagggcgcg cggcccgtcg ccgactacgg cagcgccttc
     6841 cggctcacgc ggctgagcgc cgtgccggca gcgcgacgtg accatcgagg gcgccatacc
     6901 ggcgccctcg atgagccgtc gtgcgtccgc ttacggctgg ccggcctgcg gcacggctgg
     6961 ctgctgccgc gaggccgtgc gcggggcagt ttcgcgccgc agcagcgcga ccgccgctcc
     7021 gagtgtccag agcggcaaga tcatgaacgg catgaacatg atcgcggcca gcaggatgac
     7081 cgcggccgcc aggccgaacc aggccagcca gt
//
LOCUS       DASUBI010000216         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_358005, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000216 DASUBI010000000
VERSION     DASUBI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358005"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VFU72_04205"
     CDS             complement(<1..1465)
                     /locus_tag="VFU72_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5422721.1"
                     /translation="MIALRHRFDSAPLQRPHLALPAALLLLAGLALTLGLAGGPATGL
                     LEAEARNGLLARAAAGHGGVLLNPAPGSSALLAALGALFGRALGFDVQALRLAALAGG
                     LGTALFSGLWLRRLFGAPWGAAGALVVAGSAPLLAYNRVALSPVAGALALAATLWLLG
                     EAYTRPGWRSLPWYLAAGAAGGIGFLSDPPLRLLPLLLLVPLALAIARRAPGQRTVGL
                     ALALVICDLLLSPSLPDLARSGTLGFWVPTAGLGGATITGATGLPHGYLAALARLFWP
                     PREALFGPLLLPWLLLGLLALPRRIRRPDVLLAVLWGLLLLLPAALVAPAQSGRLLAA
                     LPLLAALPLAGMRAALRRAHARWARRSLAVLAALSLGGNLAWAAVLNPTVALPPSSTE
                     QAIALANRLLGPEPVLFSTSGRDDIVDYLAATDPDGAGRLDFDGRALLPIPLGQSGYL
                     ISPADTPLDPALAALLAPLALPSLSGPAATVFRLDDRA"
     gene            1584..>2207
                     /gene="rsgA"
                     /locus_tag="VFU72_04210"
     CDS             1584..>2207
                     /gene="rsgA"
                     /locus_tag="VFU72_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5422722.1"
                     /translation="MQQPPNIVIRNYGRYYDVETPEGIVRCVPRGSLKRARRERKTDV
                     VAVGDRVEITPTGPGEGVIEAVAPRRKVLARLARGTSDVEQVILANPDQLVAVFAVAE
                     PEPHPRMLDRFLLLAEARGIPAAVVVNKIDLDESGELRRRFAPYRAAGYPVIETSART
                     GAGIEELRRLLAGKISVLTGPSGVGKSSLINDLAPERRERTGEISTAT"
BASE COUNT          328 a          801 c          866 g          212 t
ORIGIN      
        1 gggcgcggtc gtcgaggcga aagacggtcg cggcggggcc gctgagcgag ggcagggcga
       61 gcggcgccag cagcgccgcc agcgccgggt cgagcggggt gtcggccggc gagatcaggt
      121 agccactctg ccccagcggg atcggcagga gtgcgcgccc gtcgaagtcc agccgcccgg
      181 cgccgtccgg atcggtggcg gccaggtagt cgacgatgtc gtcgcgcccg ctggtgctga
      241 agagcaccgg ctccgggccg agcaggcggt tggccagcgc gatcgcttgc tcggtgctgc
      301 tcggcggaag cgcgacggtt ggattcagca cggcggccca ggcgaggttg ccgccgaggc
      361 tgagcgccgc cagtaccgcc aggctgcgcc gcgcccagcg cgcatgcgcc cggcgcagcg
      421 cggcgcgcat gccggccagc ggcagcgcgg ccagcagtgg tagcgcggcc agcagccgtc
      481 cgctctgcgc cggggcgacc agcgccgccg ggagcagcag cagcaatccc cagaggaccg
      541 ccagcagcac gtccggccgg cgaatccggc gcgggagggc cagcaggccg agtagcagcc
      601 agggcagcag cagcgggccg aagagcgcct cacgcggcgg ccagaagagg cgggcgagcg
      661 cggcgaggta gccgtgcggc aggcccgtgg cgcccgtgat cgtggcgccg cccagcccgg
      721 cggtcggcac ccagaagccg agggtcccgc tgcgtgccag gtccggcagg gacgggctga
      781 gtaacagatc gcagatcacc agcgcgagcg ccaggccgac ggtgcgctgg ccgggcgcgc
      841 gccgagcgat cgccagcgcc agcggcacca gcaggagcag cggcaggaga cgcagtggcg
      901 gatcggagag gaagccgatt ccgccggcgg ctccggctgc caggtaccag gggagcgacc
      961 gccagcccgg acgcgtgtac gcctcgccga gcagccagag cgtggccgcg agcgccagcg
     1021 cgccggccac cgggctgagc gccacccggt tataggccag cagcggcgcc gagccggcca
     1081 ccaccagcgc cccggccgcg ccccagggcg cgccgaagag gcggcgcagc cacaggccgc
     1141 taaagagcgc cgtgcccagc ccgcccgcca gcgcggccag gcgtagcgcc tgcacgtcga
     1201 agccgagcgc gcggccgaag agcgcgccga gcgctgccag cagcgcggag ctgccgggcg
     1261 ccgggttgag caggacgccg ccgtggcccg ccgcggcgcg ggccagcagc ccgttgcgcg
     1321 cctcggcctc cagtagcccg gtcgcagggc cacccgccag gcccagcgtc agcgccagcc
     1381 cggccagcag gagcagcgcg gccggcaggg cgaggtgcgg gcgctggagc ggagcagagt
     1441 cgaagcggtg acgtagcgcg atcatgatgg ccgattctag tcctgccgcg cgcgtccccg
     1501 gatagcccat cggtgctaga cttgaccggc ttgacccagg cctgtgccca cgctcacgct
     1561 cgcccgcgct ggtggagacc cgcttgcagc agccgcccaa catcgtcatc cgcaactacg
     1621 gacgctacta cgacgtcgag acgcccgagg ggatcgtgcg ctgcgtgccg cgcggttcgc
     1681 tgaagcgcgc gcggcgcgag cggaagaccg acgtggtggc ggtcggcgac cgggtcgaga
     1741 tcacgccgac cggcccgggt gagggagtga tcgaggcggt cgccccgcgc cggaaggtgc
     1801 tggcgcggct ggcgcgcggc accagcgacg tggagcaggt gatcctggcc aacccggacc
     1861 aactggtggc cgtcttcgcc gtggccgagc cggagccgca cccgcgcatg ctcgaccgct
     1921 tcctcttgct ggccgaggcg cgcgggatcc ccgccgcggt ggtcgtcaac aagatcgacc
     1981 tcgacgagag cggtgagctg cgccggcgct tcgcccccta ccgcgccgcc ggctacccgg
     2041 tcatcgagac cagcgcccgc actggcgcgg ggatcgagga gctgcgccgg ttgctggcgg
     2101 gcaagatctc ggtgctgacc ggcccctcgg gcgtgggcaa gtcgagcctg atcaacgacc
     2161 tggcgccgga gcggcgcgag cgcaccggcg agatcagcac cgccacc
//
LOCUS       DASUBI010000217        14298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_262825, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000217 DASUBI010000000
VERSION     DASUBI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262825"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3944
                     /locus_tag="VFU72_04215"
     CDS             <1..3944
                     /locus_tag="VFU72_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEU5422723.1"
                     /translation="LPALAPGDYQLRLKSGSLTGQAAIPVEDGTLVFVESDKPIYQPG
                     QTIHLRVLALDAALRPGAGQVTVEASDAKGIKVFKQTATVDDFGLAAVDLPLSDEPNL
                     GNWTVRGALGQRQGEAQLRVERYVLPKYEVTLQPAKSWVLAGEPLKGTISAEYSFGKP
                     VVGEVELVAARYVGDWQEYARLTKPLDGTLAFELPPVQYATGVPAAGGLAQVRLDVTV
                     REQATGYEEQDSQLVSVAAQPVGLRLIPESVTFKPGLPFSLLLVAETPDKQPVDTEVE
                     LEIGWERDGSPGANDTQRLATKHGLATLSLTPPRGAVLLHVLASAAQAGAVPLLVQAS
                     YSPSGAFIHLSQASQGSLKVGDTARFQVATTRETPHVYYEVTARGGVVASGVAQPPEL
                     ALPLTPLMAPEARLVVYQLLPGSVELAADYLPFSVSGDYPQAVTLAPDKDEVRPGDAL
                     ALRLQTEGPARVGLAAVDRAVFILAENRLNLQQVFDALEQLYQKPQIELHAAGPLGDF
                     GAIGGAPASMPQPAAGGIAAQPAPLVPSAPTVVPGAKELFDYAGLTVLTNRTLPEGKP
                     LASAADARKVIAGAAESVSGASAATAASQPAAIPAAAAPPAAPEPPRLRQFFPETWLW
                     DTFDSDAAGRASRDVTAPDSITTWQLRAVALSKEHGLGIGEAQLRVFQPFFVQVDLPY
                     AATRGEEFPVKLALYNYLAQPQQISVTLESAAWFDLLGAAQQALTVPANSVGAVSIPI
                     RPTALGAHGLKVTARGQQAADAIEKELLVEAEGVPRELAENLVLAAGVTRQITRDVPA
                     EAVPGSARVTLAVTGDVLSQTMDGLDALLRMPFGCGEQNMVLFAPNVFVTQYLKATAQ
                     LKTEVLAKADVMMLTGYQRELTYRRDDGSFSAFGQQDPSGSLWLTAFVLKTFAQARGL
                     LYVDDGVLAQARDWIRGHQQADGSFEPVGFVHHQELLGGLKGKAALTAFVAVALREAG
                     DDAGAAHAQRYLEGALAGLADPYGLALTSYALALGKSGQAGKARDQLMALAHESEDGL
                     YWGSPPVVLPATSYPAPPAGIPPVETTAYAVLALLELGDNLNAGRAVRWLAARRNPQG
                     GFGTTQDTVVALQAMTSAAVAGRAEIDATVSLSAGSWRKELRLTPQSADVMQLIELPL
                     DEPESVVALAVQGKGQVQAQVVRRFNLPSAADDTHSAFQIEVRYDADEVAVDDLLTIE
                     ATVTFTPPEPIAAGMTVLDLAVPTGFAAETNSLQDLQAREPRLKRFDLAGRKVIFYIE
                     DMRPNDRLILSFRARALFPVKAQAGQSQAYAYYRPEWRGEALAGALTVRGA"
     gene            4048..4308
                     /locus_tag="VFU72_04220"
     CDS             4048..4308
                     /locus_tag="VFU72_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5422724.1"
                     /translation="MNIILWIILGAIAGWIASMIMGRNASMGWLANILVGIVGAFIGA
                     LVWHLITGAAWVAGFNITPLIIAIVGAIILLFIWNLITRSRA"
     gene            4572..6773
                     /gene="recQ"
                     /locus_tag="VFU72_04225"
     CDS             4572..6773
                     /gene="recQ"
                     /locus_tag="VFU72_04225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEU5422725.1"
                     /translation="MGGSPAAIDDERLHATLAAVFGYSSFRPLQEEIIRATLDGRDVF
                     ALLPTGGGKSLCYQLPALLHPGLTVVVSPLIALMKDQVDALTALGVPATYINSSLDLD
                     ELRARQDAVARGEVRLLYVAPERLMLPGFLRLLQRVPLAGFAIDEAHCISEWGHDFRP
                     EYRELRRLRRLFSATPLAAFTATATARVEQDILEQLGLEEAARFRGSFNRANLFYEVR
                     PKRDAYRQLVSYLRDHRRDSGIIYCQSRAGTEKLAERLRADGFPAVAYHAGLESEERR
                     TRQEAFSRDQVPIVVATVAFGMGIDKPDVRFVIHYDLPKNLESYYQESGRAGRDGEPS
                     DCILFFSRGDVTKQQHFIEEKPTPEERRVAHEQLRQMADWADAHACRRRALLAYFDED
                     LAGQTGPCCDVCRSPAEEVDYTIPAQMLLSCVVRTGQRFGMNHVVDVLRGSQSQKIQQ
                     FGHERLSTYGIGRDRRKAEWLALARELVRRGYLTQDPERFNTLAVTEPGRQVLRGEET
                     VRLPKLYERGAGRADEPAGDYDDAVFEELRALRRRLAEERGVPPYVIFHDSTLRELAS
                     RLPTSQAGLRRVTGVGERKAEDFGAAVLDLLAPHAAARGVPDDEPEPDDDAGQLRFST
                     APARRTRGLSDTAQESLDLFDEGCDVAEIAQRRGLARSTIEGHLTQAIACGALADLDR
                     LVPPAKQRAIVAAVEALETARLAPLLERLGEGYSYAELRFVVTALGRERPA"
     gene            complement(7090..8160)
                     /locus_tag="VFU72_04230"
     CDS             complement(7090..8160)
                     /locus_tag="VFU72_04230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5422726.1"
                     /translation="MEVATRPIDQEKLTAFLEQMVQELAVVISSGLVVVGDRLGLYRA
                     MADGRPVSDAELAATTGVDLHYLHDWLVNQAAGGYVEYDAATDRYRLTPEQALILAGL
                     DGPLDAPGGFISMTALVQAADRIAGVMRTGGGMSWGEHHHGLFEGTERFFKGAYRTSL
                     VQQWIPALDGVAAKLRAGARVADVGCGHAASTIIMAQAYPNSRFVGFDAHAPSIEHAR
                     QAAEEAGVADRVTFAVADAASYPGQDYDLIAYFDCFHDLPAPYRSAQHSYQALAPDGT
                     VMLVEPMAGNTVAENLNPVGRFASAASVLVCRPNALATSDVALGTIAPDAAIEGMVRR
                     AGFSHFRRATETPFNRVFEARR"
     gene            8410..12105
                     /locus_tag="VFU72_04235"
     CDS             8410..12105
                     /locus_tag="VFU72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422727.1"
                     /translation="MLAPLRIQLLGDFRIQVGERAIAAEAWRLRAAALLVKLLALEPS
                     HRLHHEQLMETIWPEALPEDAGRNLRYALHAARRVLAPVGTANGGYLQRTGQWLLLEP
                     GDEGSVDVADFEAAALAARQSPDIERYAQALGLYGGDLLPGDRYEERFATRRFALRAT
                     YLALLAEFAVILRAEGRVDAAIDTLRRLLEAEPDNEAAHLALMELFVLRGEDQRALRQ
                     YDRLAEALRQVDAVPGAAARRLAGSIRPRQDEADLDVLAAGQAGGALALIVGRAAEQR
                     QLRLALEATLAGRGRVVLVSGEAGIGKTALVEDLAGQAAARGCLVLRGHAYDLSVTPP
                     YGPWLEIFRAYQPLARDLPPLPAFVESAEELARAGSQEQLFAAVAEFFAAVAARRPLL
                     LVLEDLHWADQASLAFLRVLARQVATQRILIAVTYRTDEPRQHGLYPLLPLLVREAHA
                     ERLDVPPLDERGQRALVRGRYRLDEGDADRLERYLAAHAEGHPLFAQELLRTLEQERI
                     LRLEAARWRLGELARPRVPLLLRQVIEGRLARLDQATRQLLQIAAIIGQEVPLELWQQ
                     VAEAGDEALAVAVEQGQAAHLLEEAPGEALYRFRHALFRQVLYAELNVLRRRAWHRRA
                     AEVLAAGARPDPEPVAYHYQQAGDPRAVDWLLQAGERARRAYAWLSAAERFQAALDGL
                     AEHAVPPVERAILLYRIASTLRFSDRSRALALAEEARQLAAAAGEPGLAGHCQLLAGV
                     LQCFLGEARTGVAVMEQGLVALDAIAPADLARLSARLKGISHEPAGLLAMWLAHAGRL
                     TEARSRALAMLAEDHAPVLEPGLESTGYGNMLSALAIAEALLGRPHAARRAMRRSRGA
                     NRAIHHYAEVAAMCKEELDLVQLPYFPEELEQRALLAREGDDNRQRALRTGTLIPARS
                     YALSLMVLEGNWAEAREIAVRTYDVPSWPTRPRGGRWLALLAGLQGDTALAWRIIDDL
                     FPQGSSTAPGNLVIWAALPLQRLAAELALEAGELAAARDWLRAHDRWLDWSGAVLGRA
                     EGTLLWARCYHAGGDHTPAHRQAGCALALASDPRQPLALIAVHRFLGQLETESRRLSA
                     AEEHLGQSLELADVCAAPYERALSLLELASLRLAQAQPQQAREALAGARTICERLGAG
                     PALRRVAALEEQLALTHTPRYPAGLTPREVEVLGLVAQGLTDAQVAERLFISRRTVSS
                     HLASIYNKLGVSTRTAATRFVVEHHLS"
     gene            complement(12193..13371)
                     /locus_tag="VFU72_04240"
     CDS             complement(12193..13371)
                     /locus_tag="VFU72_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422728.1"
                     /translation="MRLPVPRFVVLAVCLSLLLAACGGSASTSTTTTSTSSTAAPTAT
                     SAATATTASTPAAAASSPAASPSATTPTAAATATAGTPGAAGPLVKVELDTGFLPSMN
                     FAPYYLAAAKGYYAAQGLDVTIKDGANPGLLEQIAGGGMVFGIAGGDSLITARAAGIP
                     DVLVLQQFQKYPVGLITLANGGAAIAKPADLKGKKIGVSAPNGSTYFGLLALLQAANL
                     TKDDVTIVSIGFTELEALSQKRVDGAMTFITNEPIQARDQGIPVNQLAVSDYVNLVST
                     GLVTSEKLVAEHPDIVQKFVTASLQGLAEAQRDPDAAYAASIKRMQELSPAAAKTQRD
                     ILTATLGFEQQPSGHPYGWSDPNAWTTTINLLKSVGVITKDVDASSCYTNTFVENARP
                     "
     gene            complement(13445..14188)
                     /locus_tag="VFU72_04245"
     CDS             complement(13445..14188)
                     /locus_tag="VFU72_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU5422729.1"
                     /translation="MVAVLLSIATFVSTGLGGLFALHRRGQLCLIMAFSAGLLVAAAL
                     FDLLPEAIDMAAQSTLRTTEDVLAATAVGFLVFFGLDRLVHSLAAAHVKVGHVEMHDT
                     AAFGAIAALGLVIHSFLDGFAIGSAFRASDSIGWLVALAVIGHDFGDGVSTVSLVLGN
                     RGGLRASASLLAADALAPVLGALAALAIALPSGSLSLLLGFFAGTFLFIGASHLLPES
                     QGEGDAGPITLAVVAGFVLMYIVTQLLGA"
BASE COUNT         2050 a         5158 c         5105 g         1985 t
ORIGIN      
        1 cgctgccggc gctggcaccc ggcgactacc agctgcggct caagagcggc agcctgaccg
       61 gacaggccgc catcccggtc gaggacggca cgctcgtctt cgtcgagagc gacaagccga
      121 tctaccagcc cggccagaca atccacctgc gcgtgctggc gctcgacgcc gcgctgcgac
      181 cgggcgccgg acaggtgacg gtcgaggcga gcgacgccaa ggggatcaag gtcttcaagc
      241 agaccgccac cgtcgacgac ttcggcctgg ccgctgtcga cctgccgctc tccgacgagc
      301 cgaacctggg gaactggacg gtgcgcggcg cgctcggcca gcgccagggg gaggcgcaac
      361 tgcgcgtcga gcgctacgtg ctgcccaagt acgaggtcac gctgcagccg gccaagtcct
      421 gggtgctggc cggcgagccg ctcaagggca cgatctccgc cgagtacagc ttcggcaagc
      481 cggtggtggg cgaggtcgag ctggtcgccg cgcgctacgt cggcgactgg caggagtacg
      541 cccgcttgac caagccgctc gacggcacgc tcgccttcga gctgccgccc gtgcaatacg
      601 cgaccggcgt gccggcggcc ggcgggctgg cccaggtgcg gctcgacgtc accgtgcgcg
      661 agcaggctac cggctacgag gagcaggaca gccagctcgt cagcgtggcc gcccagccgg
      721 tcggcctgcg cctgatcccc gagagcgtca ccttcaagcc ggggctgccc ttcagcctgc
      781 tgctggtggc cgagacgccc gacaagcagc cggtcgacac cgaggtcgaa ctggagatcg
      841 gctgggagcg cgacggctcg ccgggagcga atgacaccca gcggctggcg accaagcacg
      901 gtctggccac gctcagcctg acgccgccca ggggagcggt cctgctgcac gtcctggcca
      961 gtgccgccca ggctggcgcc gtgccgctgc tggtgcaggc cagctactcc ccctccggcg
     1021 ccttcatcca cctgtcgcag gccagccagg gctcgctcaa ggtgggcgac accgcccgct
     1081 tccaggtcgc caccacgcgc gagacgccgc acgtttacta cgaggtgacg gcgcgcggcg
     1141 gcgtggtcgc tagcggtgtg gcccagccgc cggagctcgc cctgccgctg acgccgctga
     1201 tggcgcccga ggcgcgcctc gtcgtctacc agctcctccc cggcagcgtc gagctggccg
     1261 ccgactacct ccccttcagc gtcagcggcg actacccgca ggcggtgacg ctggctcctg
     1321 acaaggacga ggtcaggccg ggcgatgcgc tggcgctgcg gctgcagacc gagggtccgg
     1381 cgcgcgtcgg gctggcggcg gtcgaccgcg ccgtcttcat cctggccgag aaccggctca
     1441 atctgcagca ggtgttcgac gcgctggagc agctctacca gaagccgcag atcgagttgc
     1501 acgcggccgg gccgttgggt gatttcggcg ctatcggcgg cgctcccgcg tccatgccgc
     1561 agccggctgc cggtggcatc gccgctcagc cggcgccgct ggttcccagc gcgcccaccg
     1621 tcgtccccgg cgccaaggag ctgttcgact acgccgggct gaccgtgctg accaaccgca
     1681 cgctgccgga aggtaagccg ctggcgtcgg ccgcggacgc gcggaaggtg atcgccggag
     1741 ccgccgagtc agtctcggga gcctccgccg ccaccgcagc cagccagccc gccgccatcc
     1801 cggcagccgc ggcgccgccc gccgcgcccg agccgccgcg cctgcgccag ttcttccccg
     1861 agacctggct ctgggacacc ttcgacagcg acgccgccgg ccgcgccagc cgcgacgtga
     1921 ccgcgcccga cagcatcacc acctggcaac tgcgcgcggt ggcgctctcg aaagagcacg
     1981 ggctgggcat cggcgaggcg cagctgcgcg tcttccagcc cttcttcgtg caggtcgatc
     2041 tcccctacgc ggcgacgcgc ggcgaggagt tcccggtcaa gctggcgctc tacaactatc
     2101 tggctcagcc gcagcagatc agcgtgacgc tggagagcgc cgcctggttc gacctgctcg
     2161 gcgcggcgca gcaggcgctg accgtgccgg ccaacagcgt cggcgcggtc tcgatcccga
     2221 tccggcccac ggcgctcggc gcgcacgggc tgaaggtgac ggcgcgcggc cagcaggccg
     2281 ccgacgccat cgagaaggag ctgctggtcg aggccgaggg cgtgccgcgc gagctggccg
     2341 agaatctggt gctggcggcc ggggtcacgc gccagatcac gcgcgacgtg ccagccgagg
     2401 ccgtgcccgg ctcggcccgc gtcacgctgg cggtcaccgg cgacgtgctc agccagacga
     2461 tggacgggct ggatgcgctg ctgcgcatgc ccttcggctg cggcgagcag aatatggtcc
     2521 tcttcgcgcc caacgtcttc gtgacccagt acctgaaggc gaccgcgcag ctcaagaccg
     2581 aggtgctggc caaggccgac gtcatgatgc tgaccggcta ccagcgcgag ctgacctacc
     2641 ggcgcgacga cggctccttc tccgccttcg gccagcagga tccctccggc agcctctggc
     2701 tgaccgcctt cgtgctcaag accttcgccc aggcgcgcgg cctcctctac gtcgacgacg
     2761 gcgtgctggc ccaggcgcgc gactggatcc gcgggcacca gcaggccgac ggcagcttcg
     2821 agccggtggg cttcgtccac caccaggagt tgctgggcgg gctcaagggc aaggcggcgc
     2881 tgaccgcctt cgtggccgtg gccctgcgcg aggccggcga cgacgcgggc gcggcccacg
     2941 cccaacgcta tctcgaaggc gcgctggccg gcctcgccga cccctatggc ctggcgctga
     3001 ccagctacgc gctggcgctg ggcaagagcg gccaggccgg caaagcgcgc gaccagctca
     3061 tggcgctggc tcacgagagc gaggacggcc tctactgggg cagcccgccg gtggtgctgc
     3121 ccgcgaccag ctacccggca ccgccggccg gcatcccgcc ggtcgagacg accgcctacg
     3181 ccgtgctggc gctgctcgag ctcggcgaca acctcaacgc cggccgcgcc gtgcgctggc
     3241 tggcggcgcg gcgcaacccc cagggcggct tcggtacgac ccaggacacc gtcgtcgcgt
     3301 tgcaggccat gaccagcgcc gccgtcgccg gccgcgccga gatcgacgcc accgtgtcgc
     3361 tgagcgccgg gagctggcgt aaagagttgc gcctgacgcc gcagagcgcc gacgtgatgc
     3421 agttgatcga gctgccgctc gacgagccgg agagcgtggt ggcgctggcg gtgcagggca
     3481 aggggcaggt gcaggcccag gtagtgcgcc gcttcaacct gcccagcgcg gcggacgaca
     3541 cccattccgc cttccagatc gaggtgcgct acgacgccga cgaggtggcg gtcgacgacc
     3601 tgctgaccat cgaggcgacg gtgaccttca ccccgccgga gccgatcgcg gccggcatga
     3661 cggtgctcga cctggccgtg ccgaccggct tcgccgccga gaccaattct ctgcaagatc
     3721 tgcaggcacg cgagccgcgt ctcaagcgct tcgacctggc cggacgcaag gtgatcttct
     3781 acatcgagga catgcgaccc aacgaccggc tcatcctcag cttccgggcg cgggcgctct
     3841 tcccggtcaa ggcgcaggcg ggtcaatcgc aggcctacgc ctactaccgg cccgaatggc
     3901 gcggcgaggc gctagccggg gcgctgacgg tgcgcggcgc gtgacaggtg gcacagcggt
     3961 tgcatagagg ggcgtaggac cggaggcgag gtccgagccg ttgggccggg ggacatgacc
     4021 cacccggctg aaacaaggag gtcgttcgtg aacattatct tatggatcat cctgggcgcg
     4081 atcgccggct ggatcgccag tatgatcatg ggccgcaacg cgtcgatggg ctggctggcc
     4141 aacatcctcg tcgggatcgt gggcgccttc atcggcgcgc tggtctggca cctgatcacc
     4201 ggcgcggcct gggtggcggg cttcaacatc accccgctga tcatcgccat cgtcggcgcg
     4261 atcatcctgc tcttcatctg gaacctgatt acccgcagtc gcgcctaacg gcatcacctc
     4321 caaccatctc agtggaacga ccccggcccc gccggggtcg tcgcatttgc acggggtggc
     4381 cctcatcccc ccgccccttc tcccaatact gggagaaggg gagcaacgta cgggagcacg
     4441 atagtgctcc ccgctcccaa tcctgggagc ggggatgggg gtgagggctc gtccgaacgc
     4501 atgttcgcaa cccatccaac ctgctacact tcaagcggat gaacgtacgg cgcgacggag
     4561 agaggcagac catgggcggc agtcccgcgg cgatcgatga cgagcggctc cacgccacgc
     4621 tggccgccgt cttcggctac tcctccttcc gcccgctgca agaggagatc atccgcgcca
     4681 cgctcgacgg gcgcgacgtc ttcgccctgc tgccgaccgg tggcggcaag tcgctctgct
     4741 accagcttcc cgccctctta caccccggcc tgaccgtggt cgtctcgccg ctgatcgcgc
     4801 tgatgaagga tcaggtcgat gccctgaccg cgctcggcgt gccggccacc tacatcaaca
     4861 gctcgctcga tctcgacgag ttgcgcgcgc gccaggacgc cgtggcgcgc ggcgaggtgc
     4921 ggctcctcta cgtcgcgccc gagcggctga tgctgccggg cttcctgcgc ctcttgcagc
     4981 gcgtgccgct ggccggcttc gccatcgacg aggcgcactg catctccgag tggggccacg
     5041 acttccgccc cgagtatcgc gagctgcgcc ggctgcgccg gctcttctcg gccaccccgc
     5101 tagccgcctt caccgccacc gcgaccgcgc gggtggagca ggacatcctg gagcagctcg
     5161 ggctcgaaga ggccgcgcgc ttccgcggca gcttcaaccg cgccaacctc ttctacgagg
     5221 tgcgacccaa gcgcgacgcc taccgccagc tcgtgagcta cctccgggac caccggcgcg
     5281 actccggcat catctactgc cagtcgcgcg ccggcaccga gaagctggcc gagcgcctgc
     5341 gggccgacgg cttcccggcg gtcgcctatc acgcggggct ggagagcgag gagcgccgca
     5401 cgcggcagga ggccttcagc cgcgaccagg tgccgatcgt ggtcgcgacc gtcgccttcg
     5461 gcatggggat cgacaagccc gacgtgcgct tcgtgatcca ctacgaccta cccaagaacc
     5521 tggagagcta ctaccaggag agcggccgcg ccgggcgcga cggcgagccg agcgactgca
     5581 tcctcttctt cagccgcggc gacgtgacca agcagcagca cttcatcgag gagaagccga
     5641 cgccggagga gcggcgcgtc gcccacgagc aactgcgcca gatggccgac tgggccgacg
     5701 cccacgcgtg ccgccgccgc gccttgctcg cctacttcga cgaggatctc gccgggcaga
     5761 ccggcccctg ctgcgacgtc tgccgcagcc cggccgagga ggtcgactac accatcccgg
     5821 cgcagatgct cctctcctgc gtggtgcgca cgggccagcg cttcggcatg aaccacgtgg
     5881 tggatgtctt gcgcggctcg cagtcgcaga agatccagca gttcggccac gagcgcctct
     5941 cgacctacgg catcggccgc gaccggcgca aggccgagtg gctggcgctg gcgcgcgagc
     6001 tggtgcggcg cggctacctg acgcaggacc ccgagcgctt caacacgctg gccgtgaccg
     6061 agcccggccg gcaggtgctg cgcggcgagg agacggtgcg gttgccgaag ctctacgagc
     6121 gcggcgccgg gcgcgcggac gagccggcgg gcgactacga cgacgccgtc ttcgaggagc
     6181 tgcgcgcgct gcgtcgccgg ctggccgagg agcgcggcgt gccgccctac gtcatcttcc
     6241 acgacagcac cctgcgcgag ctggccagcc ggctgccgac gagccaggcc gggctgcggc
     6301 gcgtcaccgg cgtcggcgag cgcaaggccg aggacttcgg cgcagccgtg ctggacctgc
     6361 tggcgccgca cgccgccgcc cgcggcgtgc cggacgacga gcccgagccg gacgatgacg
     6421 cgggccagct gcgcttctcc acggcgccgg cccgccgcac gcggggtctg agcgacaccg
     6481 cccaggagag cctcgacctc ttcgacgagg gctgcgacgt ggccgagatc gctcagcggc
     6541 gtgggctggc gcgctccacc atcgaggggc acctgaccca ggcgatcgcc tgtggcgcgc
     6601 tggccgacct cgaccgcctc gtcccgcccg cgaagcagcg ggcgatcgtc gccgccgtcg
     6661 aggcgctcga gaccgcccgc ctcgccccgc tgctggagcg cctcggcgag ggctacagct
     6721 acgccgaatt gcgcttcgtc gtcaccgcgc tgggccgcga gcggccggcg taaccggcga
     6781 cgccgggtcg agcgaccgtg cgagcctcgt caggcgccgc gccagcgagc gacgcaccgg
     6841 gaaggacgaa ttgctcggcg aggggatcgg catctccccg gccgccgact gccgcgcgga
     6901 ccaatagaag aaggcctggt catgcgcgcg gagccggtcg cttgccgacc ggtcaagcgg
     6961 cacgactgcc cgccgcgttc gacgagagag atagaaatct gccactcgga catacgggtc
     7021 catgttgacc tcctgactcc gcgagcggca cagtcatccc gcgccgcgcc gaaggccccg
     7081 gtcttgccgt cagcgcctgg cctcgaagac gcggttgaag ggcgtctcgg tggcgcggcg
     7141 gaagtggctg aagccggcgc ggcgcaccat cccttcgatc gccgcgtccg gcgcgatcgt
     7201 gcccagcgcc acgtcgctgg tggccagcgc gttcggccgg cagaccagca ccgaggcggc
     7261 cgacgcgaag cgaccaaccg ggttgaggtt ctcggccacg gtattccccg ccatcggctc
     7321 gaccagcatc accgtgccgt ccggcgccag cgcctgatag ctgtgctggg ccgagcgata
     7381 gggcgccggc agatcgtgga agcagtcaaa gtaggcgatc aggtcgtagt cctggcccgg
     7441 gtaggacgcc gcgtcggcca ccgcgaaggt gacgcgatcc gccacgcccg cttcttccgc
     7501 cgcctggcgc gcatgctcga tcgacggcgc gtgcgcgtcg aagccgacga agcgcgagtt
     7561 ggggtaggcc tgggccatga tgatggttga ggcggcgtga ccgcagccga cgtcggccac
     7621 ccgcgcgccg gcccgcagct tggccgccac gccgtcgagc gccgggatcc actgctgcac
     7681 caggctggta cggtaggcgc ctttgaagaa gcgctcggtg ccctcgaaga gcccgtggtg
     7741 gtgctcgccc caggacatac cgccgcccgt gcgcatcacc ccggcgatgc ggtcggccgc
     7801 ctgcaccagc gccgtcatcg agatgaagcc gcccggcgcg tcgagcgggc cgtccaggcc
     7861 ggccaggatc agcgcctgct ccggggtcag ccggtagcgg tcggtggcgg cgtcgtactc
     7921 gacgtagccg ccggccgcct ggttgaccag ccagtcatgg agatagtgca ggtcgacgcc
     7981 ggtcgtcgcg gccaactcag cgtcgctcac cggccggccg tcggccatgg cgcgatagag
     8041 cccgagcctg tccccgacca ccaccaggcc ggagctgatc acgaccgcga gctcctgaac
     8101 catctgctcg agaaacgccg tgagcttctc ctggtcgatc ggtcgtgtcg cgacttccat
     8161 cggagcgccc ctctctccta acaccgatac cgctgccggc ccagcgcgcc ggcaggctac
     8221 gtgttgatcc gctgccacac caccctagcg cggcgacgtt cgcgcgccgt ttgccgggga
     8281 ctacgtacgc acacgcggca cacgtacgta gtgacttgac gcagggggaa aaaggaggat
     8341 actgaagtcg gcaaataccc tcttgcacga tcatgtgaat ccgcggcgga actggtgatg
     8401 gaattggacg tgctggctcc actccgcatt cagctgctcg gcgactttcg cattcaggtc
     8461 ggagaacgag cgatcgccgc cgaagcctgg cgcctgcggg cggccgcgct gctggtcaag
     8521 ctgctggcgc tggagccgtc gcaccgcctg catcacgagc agttgatgga gacgatctgg
     8581 cccgaggcgc ttccggagga tgccggccgc aacctgcgct acgccctgca cgccgcgcgg
     8641 cgggtgcttg ctcccgtggg aacggccaac ggcggctatc tccagcgtac gggccagtgg
     8701 ctgctgctcg agcccggcga cgagggcagc gtcgatgtcg ccgacttcga agcggcggcg
     8761 ctggccgcgc gccagtcgcc cgacatcgag cgctacgccc aggcgctggg gctctacggc
     8821 ggcgatctcc tccccggcga ccgctacgaa gaacggttcg cgacccgccg gttcgcgctg
     8881 cgcgccacct acctcgcgct gctcgccgaa ttcgcggtga tcctgcgcgc cgagggccgg
     8941 gtcgacgccg cgatcgacac cctgcggcgg ctgctggagg ccgagccgga caacgaggcg
     9001 gcccacctgg cgctgatgga gctgttcgtg ctgcgcgggg aggaccagcg cgcgctgcgg
     9061 cagtacgacc ggctcgccga ggcgctgcgc caggtggacg ccgttcccgg cgccgccgct
     9121 cgccgcctgg cgggctccat ccgcccgagg caggacgagg cggacctgga cgtgctcgcg
     9181 gccggccagg ccggcggcgc gctggcgctg atcgtgggtc gcgccgccga gcagcggcaa
     9241 ctgcggctgg ccctggaggc cacgctggcg gggcgcggcc gggtcgtgct ggtgagcggc
     9301 gaagccggca tcggcaagac ggcactggtg gaggatctcg cgggccaggc cgccgcgcgc
     9361 ggctgcctcg tcctccgtgg ccatgcctac gatctctcgg ttacgccgcc ctacggcccc
     9421 tggctggaga tcttccgcgc ctaccagccg ctggcgcggg atctgccgcc gctccccgcc
     9481 ttcgtcgaga gcgccgagga gctggcccgg gccggcagcc aggagcaact gttcgcggcg
     9541 gtggccgagt tcttcgccgc cgtcgccgcg cggcgcccct tgctgctggt gctggaggat
     9601 ctgcactggg ccgatcaggc cagcctcgcg ttcctgcgcg tgctggcgcg gcaggtcgcc
     9661 acccagcgca tcctcatcgc ggtcacctat cgcacggacg agccgcggca gcacgggctc
     9721 tacccgctgc tgccgctcct ggtccgggaa gcgcatgccg agcggctgga cgtaccccca
     9781 ctggacgagc gcgggcagcg cgccctggtc cgcggccgct accggctgga cgagggcgac
     9841 gcggaccggc ttgagcgcta cctcgccgcg cacgccgagg ggcatccgct cttcgcgcag
     9901 gagcttctgc gaacgctgga gcaggagcgt atcctgcggc tggaggctgc gcgctggcgc
     9961 ctgggtgagc tggcgcggcc gcgcgtgccg ctgctgctgc gccaggtgat cgaggggcgg
    10021 ctggcacggc tcgaccaggc cacacgccaa ctgctgcaga tcgcggcgat catcggtcag
    10081 gaggtgccgc tggagctgtg gcagcaggtg gccgaggcgg gcgacgaggc gctggcggtg
    10141 gccgtcgagc aggggcaggc ggcccatctc cttgaggagg cgcccggcga agcgctctat
    10201 cgtttccgcc acgccctctt ccgtcaggtg ctctacgccg agctgaacgt gctgcggcgg
    10261 cgcgcctggc accggcgggc ggccgaggtg ctggcggccg gcgcgcggcc ggacccggag
    10321 cctgtcgcct accactatca gcaggcgggc gacccgcgtg ccgtcgattg gctgctccag
    10381 gcgggcgagc gcgcgcgccg tgcctacgcc tggctcagcg ccgccgagcg cttccaggca
    10441 gcgctggatg ggctggccga gcacgccgta ccccccgtgg agcgtgcgat cctcctctac
    10501 cgcatcgcga gcacgctccg cttctcggac cggagccgcg cgctggcgct ggcggaggag
    10561 gcgcgtcagt tggcggccgc ggcgggtgag ccgggtttgg ccggccactg tcagttgctc
    10621 gcgggggtac tccagtgctt cctgggagaa gcccgcacgg gggtggccgt gatggagcag
    10681 gggctcgtgg cgctcgatgc catcgcccca gccgatctgg cgcggctctc ggcccgcttg
    10741 aaaggcatct cgcacgagcc ggccggcctg ctggccatgt ggctggccca tgccgggcgc
    10801 ctgacggaag cgcgctcgcg cgcgctggcg atgctggcgg aagatcacgc cccggtgctc
    10861 gagccgggcc tggaatccac gggctacggc aacatgctct cggcgctggc catcgccgag
    10921 gcgctgctcg gtcgcccgca cgccgcccgg cgcgcgatgc ggcgctcccg gggcgccaac
    10981 cgcgcgatcc accactacgc cgaggtggcc gccatgtgca aagaggagct ggacctggtg
    11041 cagcttccct acttccccga agagctggag caacgcgcgc tcctggcgcg cgagggcgac
    11101 gacaaccggc aacgcgcgct gcgcaccggc acgctgattc cggcgcgatc ctatgccctc
    11161 tccctcatgg tgctggaggg gaactgggcg gaggcgcgcg agatcgccgt gcggacctac
    11221 gacgtgccgt cctggcccac gcgcccgcgc ggcggccgct ggctcgcgct gctggccggg
    11281 ctgcagggcg acacggccct ggcgtggcgg atcatcgacg atctcttccc ccaaggctcg
    11341 tcaaccgcac cgggcaatct ggtcatctgg gccgcgctgc cgctgcagcg cctggcggcc
    11401 gagctggcgc tggaggcggg cgagctagcc gccgcccgcg actggctacg ggcccacgac
    11461 cgctggctcg actggtccgg cgcggtgctc ggccgggccg agggtaccct cctctgggcg
    11521 cgctgctacc acgccggcgg cgaccacacg ccggcccacc ggcaggccgg gtgcgcgctc
    11581 gccctcgcct ccgatccccg ccagccgctg gccctgatcg ccgtccaccg tttcctgggg
    11641 cagctcgaga cggagtcgag gcgcctcagc gccgccgagg agcacttggg ccagtcgctg
    11701 gagctagcgg acgtctgtgc cgcaccctat gaacgcgcgt tgagcctgct ggaattggcg
    11761 agtctgcggc tggcacaggc acagccgcag caggcgcggg aggcgctggc cggcgcccgg
    11821 acgatctgtg agcgactggg cgccggaccg gcgctgcggc gcgtcgccgc gctggaggag
    11881 cagctcgcgc tcacacacac gccccgctac ccggcggggt tgaccccgcg cgaggtggag
    11941 gtgctggggc tggtggccca ggggctgacc gacgcccagg tggccgagcg gctcttcatc
    12001 agccgacgga ccgtcagcag ccatctggcc tccatctaca acaagctcgg cgtctcgact
    12061 cgaaccgccg ccacccgctt cgtggtcgag catcacctgt cgtaagcgac cgtgcgcctc
    12121 actccgcggc tggagcgcct gggtgagggc aaacacggcg gactgccctc cagcatctac
    12181 ggaccagcga tcttacggcc tggcgttctc cacgaacgtg ttggtgtagc agctcgacgc
    12241 gtcgacgtcc ttggtgatca cgccgaccga cttcagcagg ttgatcgtgg tcgtccaggc
    12301 gttggggtcg ctccaaccgt agggatgacc cgacggctgc tgctcgaagc cgagcgtggc
    12361 ggtcaggatg tcgcgctgcg tcttggccgc ggccgggctc agctcctgca tgcgtttgat
    12421 cgacgctgca tacgccgcgt cggggtcgcg ctgcgcctcg gccagcccct gcagcgaggc
    12481 ggtcacgaac ttctgcacga tatccgggtg ctcggcgacc agcttctcgc tggtcaccag
    12541 cccggtcgag accagattga cgtaatccga gacggccagc tgattgaccg ggatgccctg
    12601 gtcgcgcgcc tggatcggct cgttggtgat gaaggtcatg gcgccgtcga cccgcttctg
    12661 cgacagcgcc tccagctcgg tgaagccgat gctgacgatg gtgacgtcgt ccttggtcag
    12721 gttggccgcc tgcaggagcg ccagcagccc gaagtaggtc gagccgttgg gcgccgagac
    12781 gccgatcttc ttgcccttga ggtcggcggg cttggcgatt gcggcgccgc cgttggccag
    12841 cgtgatcagc ccgaccgggt acttctggaa ctgctgcagc accagcacgt cggggatgcc
    12901 ggccgcgcgc gccgtaatca gcgagtcgcc gccggcgatg ccgaagacca tcccgccgcc
    12961 agcgatctgc tccagcaggc cggggttggc gccgtccttg atggtgacgt cgagcccctg
    13021 ggcggcgtag tagcccttcg cggccgccag gtagtagggc gcgaagttca tgctcggcag
    13081 gaagccggtg tccagttcca ccttgaccag cgggccggcc gcgccgggcg tgccggccgt
    13141 ggctgtcgcg gcggccgtcg gggtggtggc gctggggctg gcggccgggc tgctggcggc
    13201 cgcggcgggc gtgctggctg tggtggccgt cgccgcgctc gtcgcggtcg gggcggcggt
    13261 gctcgatgtc gaggtcgtgg tggtgctggt cgaggccgag ccgccgcagg cggccagcag
    13321 caggctcagg cagacggcga gcacgacgaa gcgggggaca ggcagacgca tagctcctcc
    13381 tcatgctgtc cccgcgccac cagacgtcgc gggggcgttg ggcaggatag cacaggacga
    13441 tgcgtcaggc cccgagcaat tgggtcacga tatacatcag gacgaagccg gccacgaccg
    13501 ccagtgtgat cggcccggcg tcgccctcgc cctgcgactc gggcagcagg tgtgaggcgc
    13561 cgatgaagag gaaggtgccg gcgaagaagc cgagcaggag gctcaggctg ccgctgggga
    13621 gcgcgatcgc cagcgccgcc agcgccccga gcaccggagc cagcgcgtcg gcggccagca
    13681 ggctcgccga ggcccgcagc ccgccgcggt tgcccagcac caggctgacc gtcgagacgc
    13741 cgtcgccgaa atcgtggccg atcacggcca gcgcgaccag ccagccgatc gagtcgctgg
    13801 cgcggaaggc gctgccgatg gcgaagccgt cgaggaagga gtgaatcacc aggccgagcg
    13861 ccgcgatggc gccgaaggcg gcggtgtcgt gcatctcgac gtggcccacc ttgacgtgcg
    13921 cggcggccag gctatggacc aggcgatcga gcccgaagaa gacgaggaag ccgaccgcgg
    13981 tcgcggccag cacgtcttcg gtcgtccgca gcgtcgattg cgcggccata tcgatagcct
    14041 cgggcagcag atcgaagagc gccgccgcca ccagcagtcc ggccgagaag gccatgatca
    14101 ggcagagctg gccccggcgg tgcagggcga agaggccgcc cagcccggtg gagacgaagg
    14161 tcgcgatcga caacagaaca gctaccatgg atttcctctc gcgccctcgc tccacgggca
    14221 gcacgaccgc tgcccggcgg ccccgcccac cccgtatgat agagcctgtc gagcgagcgc
    14281 gtggcgatgg ccagtgta
//
LOCUS       DASUBI010000218         6797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_794430, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000218 DASUBI010000000
VERSION     DASUBI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794430"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..219)
                     /locus_tag="VFU72_04250"
     tRNA            complement(147..219)
                     /locus_tag="VFU72_04250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:Arg,seq:tcg)
     gene            468..797
                     /locus_tag="VFU72_04255"
     CDS             468..797
                     /locus_tag="VFU72_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5422730.1"
                     /translation="MMEQKPGSLAGLGRFSEPGLFILISLAGGPKHGYAMMEDIARLT
                     QSQIGPGTLYGALSRLEQQGLIEALPPEERRRPYRLTERGADVLRHTLTTMQSLATAG
                     QARLAQA"
     gene            794..1231
                     /locus_tag="VFU72_04260"
     CDS             794..1231
                     /locus_tag="VFU72_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422731.1"
                     /translation="MKRLVYLYPRRWRARYGDEFMALLEDCPRRPGVVLDVLRGCGEA
                     HWTAMTSRDWSASAMLRSSAQVLAALFGFVVCYIMLSIAIHFVAVAVISPLMMEPGLH
                     TVAPWVAQLLFGVIPLLLALSVTVVGMLRLRRRLSRKLAVGDA"
     gene            1900..2571
                     /locus_tag="VFU72_04265"
     CDS             1900..2571
                     /locus_tag="VFU72_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEU5422732.1"
                     /translation="MAVDENQIATIGESIIGRLERVNEARERALAETRRIVRLSANAI
                     RAVHRNEFDQAESLLAEARALQDGLAGALADHPNIYWSGYVQDAQKEYAEACITLGII
                     SGSGVPEPAALNVEDAVYLNGLGEAVGELRRYALDALRRDDLARAESLLPTMDEIYGL
                     LVSVDFPDAVTGGLRRTTDMVRGVLERTRGDLTLSRQQHELEKAIVRALDRLGDGEER
                     AASGV"
     gene            2687..5602
                     /gene="gcvP"
                     /locus_tag="VFU72_04270"
     CDS             2687..5602
                     /gene="gcvP"
                     /locus_tag="VFU72_04270"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU5422733.1"
                     /translation="MGATKTAIPSLDYTDRFENRHIGPRPDQIEKMLSYLGFNTLDDL
                     IDAAIPRQIRTARPLSFAGIGGPVGEESRSEYEALADLKALASRNKMYRSYIGMGYYN
                     TITPPVILRNIVENPGWYTQYTPYQAEIAQGRLEALINFQTVVTDLTGLEIANASLLD
                     EATAAAEAMSMAHAIKVRKGNPAFFVSDECHPQTVAVVQTRAGALDIPVIVGDWRTFA
                     FDQEVFGVLLQYPTTYGAVEDYGPFIEKAHAAGALAVVAADILSLTLLRPPAEFGADI
                     AVGTTQRFGVPLGYGGPHAAYLSTKDEYKRQIPGRICGISIDAEGKPAVRLALQTREQ
                     HIRREKATSNICTAQVLLAVVAGMYAVWHGPEGLKEIARRVHLLTATLDAGLKRLGYD
                     TGDQLYFDTLRIDTGKTAARAIIDRALNHEINLRPIDDHTVGISLDQVTSQEDVAELL
                     TIFNGGQPLDFELGEIVMTLDTSYPGSHARTSPYLEHPVFNTHHSETEMLRYITRLEH
                     RDLSLNYSMIPLGSCTMKLNATAEMIPVTWPEFGGIHPYVPEGQTPGYHEMMIRLEKM
                     LAEITGFDEVSLQPNAGSQGEYTGLLVIREYQKSRGQEHRDICLIPSSAHGTNPASAT
                     MAGFKVVVVKCDNEGNIEVDDLRAKAEQHQENLAALMVTYPSTHGVFEESIVEICDIV
                     HQHGGQVYMDGANMNAQVGLCRPAEIGADVCHLNLHKTFCIPHGGGGPGMGPIGVKSH
                     LAPFLPTHPVVHIGGPEGIGPVAAAPFGSPSILTISYAYISMMGAHGLTEATKVAILN
                     ANYIAKRLDPHYPVLYKGKNGMVAHECIVDLRQLKERTGVEVADAAKRLMDYGFHAPT
                     ISFPVPGTMMIEPTESEDQGEMDRFCDAMIAIRDEIAEIELGMADREDNLLKNAPHTA
                     LMIASDEWAHPYSRMKAAYPAHWLIGNKFWPPVARINDVYGDRNLVCACPPVETYAD"
     gene            5714..6715
                     /locus_tag="VFU72_04275"
     CDS             5714..6715
                     /locus_tag="VFU72_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU5422734.1"
                     /translation="MAGKTVYGGQAVMEGVMMRGRDSMAVAVRAPDQSIVVYHEPLAA
                     SPWALRVKNWPLVRGVVMLWDTMVLGTRALVFSANIGLTEEAEEDQEPSALAGPALWG
                     TVAISILFSIGLFFLLPLLVVHLIDRYIDSSVVSNLVEGLIRLGLLLGYLGLLGNLRD
                     IRRVFGYHGAEHKTINALEAGDPLDVEHVRRHTLLHPRCGTGFLLVVAVISVIVFAFL
                     GRPDFVLRVLSRILLVPVIAAIAYEFLKFTAKHDRNPIVRTVIAPSLAMQRLTTREPD
                     DEMLQVAIVSLQRVLVAEGALSAATLARPGIREVDIDGHVAGQPGAAIEPTPALGID"
BASE COUNT         1140 a         2270 c         2261 g         1126 t
ORIGIN      
        1 cgccggagct ggcgcgcgaa gcaatcgcgg ccaccgacgc cgcgctcggt tcctgagaac
       61 accttggcta cacgagacgc gaatccggcc cggcgctctc gtcgggccgt tcccattttg
      121 cgcgttcagt caacgatttc ggctagtacc ctcggaggga ctcgaacccc caaccccttg
      181 atccgaagtc aagtgctctg tccgttgagc tacgagggcc tcttcggatt ttcgcgtgtg
      241 gatacggcgc cttaccgctg accccgcgac ctgccttgcc gcaacaggct cgctgcacgc
      301 cacgattcca cacggaaata ttaccacgga cctcgcgcca gcctgccgtt cagctgtccg
      361 catccgagtg atgtgacagc cgcagtagtt ggcgtgcccc ttgacggcga cggccgcgtg
      421 agctattatt acgtcgaacg atatagcgga ctacgtagta gagggtaatg atggagcaaa
      481 agccgggaag tttggccggg cttggccggt tttccgagcc agggctcttt atcctcatca
      541 gcctcgcggg tggtccgaag catggctacg ccatgatgga ggacatcgcg cggttgaccc
      601 agtcgcagat cggtcccggc acgctctatg gcgcgctgtc gcgcctggag cagcaagggc
      661 tgatcgaagc gctgccgccg gaggagcggc gcagacccta ccgcctgacc gagcgtgggg
      721 ccgacgtact gcgacacaca ctgaccacca tgcagagcct agccacggcc ggacaagcgc
      781 ggttggccca ggcatgaagc ggctggtcta cctctacccc cgtcgctggc gcgcccgcta
      841 cggcgacgag ttcatggccc tgttggagga ctgcccaagg cgtcctggcg tcgtgctgga
      901 cgtgctgcga ggctgcggag aggcccactg gacggcaatg acaagccggg attggagtgc
      961 ttcagctatg ttgcgtagct ctgcacaggt gttggcggcg ctctttggtt tcgtggtgtg
     1021 ttacatcatg ctgagcatcg cgatccactt tgtcgctgtc gctgttatct ctccactcat
     1081 gatggagccg gggttgcaca cggtcgcgcc gtgggtggcg cagctcttgt tcggcgtcat
     1141 cccgctgttg ctggcgctct ccgtcaccgt cgttggcatg cttcggcttc ggagaaggtt
     1201 gagccggaag cttgcggtcg gcgatgcctg atagcgcgag tgagaacggc cgtcggcggt
     1261 gagtctgagc ctctccattg cgtgtagcgc gatacggttg ctggtgcggc ggctcgtggt
     1321 cgacccggac gggacggcgt gtttgatacg catggtcgta tccccgctgc ctggcctggt
     1381 tgagggctcg aatcgcgttt ccggtgatct ggcggtgtgc catgctcaac ccgggcagcc
     1441 ccgaggagtc ggcgcgggag cgcggcgcgg cgcgattggt tcgcgaggat cggccacttg
     1501 ctggtctcgg tcgtcctggg cttgcccagc ttgctctcca tcgcctgggg gctctacctg
     1561 gttttgatcc cgctgcaccg cggggtctat cgggatagta cgaacctgtt ctacgtggtc
     1621 cttggtctcg tcgtcctgac cgtcggagcg tcttcgccgg cttcgcggtc tggatcgttc
     1681 gggccgccgg tccttccaag acgaagaacg acgacaacag ctaatggaag cgtgcacgtg
     1741 atccgcaggc cacgtgattg accgtcgcgc cacgacggcc ggcgcacgag cgcgcccgca
     1801 acaccccatc tcgtgtgatt ataagaacgg cagttagccc ggccggatcg ggctggccac
     1861 gcctgcagtg gggtgtgttc gagcagagtg gggtgttcca tggcggtgga cgagaatcag
     1921 atcgcgacga tcggtgagtc gatcatcggg cggctggagc gggtcaacga ggcgcgcgag
     1981 cgggcgctgg cagagaccag gcgcatcgtg cgcctgagcg ccaacgcgat ccgcgccgtc
     2041 catcgcaatg agtttgacca ggcggagtcg ctgctggccg aggcgcgcgc gctgcaggat
     2101 gggctggccg gcgcgctggc cgaccacccc aatatctatt ggtcgggcta cgtgcaggac
     2161 gcacagaagg agtacgccga ggcgtgcatc acgctgggga tcatcagcgg ctccggcgtg
     2221 cccgagccgg cggcgctcaa cgtcgaagac gccgtctacc tcaacggcct cggcgaggcg
     2281 gtcggcgagc tgcgccgcta cgcgctcgac gccctgcggc gcgacgatct ggcgcgcgcc
     2341 gagtcgctcc tgcccaccat ggacgagatc tacggcctgc tcgtctcggt cgacttcccc
     2401 gacgcggtca ccggcggcct gcgccgcacc accgacatgg tgcgcggggt gctggagcgc
     2461 acgcgcggcg acctcaccct ctcgcggcag cagcacgagt tagaaaaggc gatcgtgcgc
     2521 gcgctcgacc ggctgggcga cggcgaggag cgtgccgcga gcggcgtgtg agcgggggct
     2581 attggcactg acgcccgcat gccaaacgcg caactgggtg taagattagc agttggacgc
     2641 caccagaccg gcgcacgggt cagacgacgc ctacagggag tgagacatgg gggccaccaa
     2701 gaccgcgatt ccgtcacttg actacaccga tcggttcgaa aaccggcaca tcgggccgcg
     2761 tccggaccag atcgaaaaga tgctcagcta tctgggcttc aacacgctcg acgacctgat
     2821 cgacgcggcc atcccgcgcc agatccgcac cgcacgcccg ctctccttcg cggggatcgg
     2881 tgggccggtc ggggaggaga gccgcagcga gtacgaggcc ctggcggacc tcaaggcgct
     2941 ggcgtctcgg aacaagatgt atcgcagcta cattggcatg ggctactaca acaccatcac
     3001 cccgccggtg atcctgcgca acatcgtgga gaaccccggc tggtataccc agtacacgcc
     3061 ctatcaggcc gagatcgccc aggggcgact ggaggcgctg atcaacttcc agacggtggt
     3121 caccgacctg accgggctgg agatcgccaa cgcctcgctg ctcgacgagg ccaccgcggc
     3181 ggccgaggcc atgtcgatgg cccatgccat caaggtgcgc aagggcaacc cggcgttctt
     3241 cgtctccgac gagtgtcacc cgcagaccgt ggccgtggtt cagacccgcg ccggcgcgct
     3301 cgatatcccc gtgatcgtcg gcgactggcg caccttcgcc ttcgaccagg aggtcttcgg
     3361 cgtgctgctg cagtacccga cgacctacgg cgcggtcgag gactacgggc cgttcatcga
     3421 gaaggcgcac gcggccggcg cgctggccgt cgtggccgcc gacatcctaa gcttgacgct
     3481 gctgcgcccg ccggccgagt tcggcgcgga catcgcggtc ggcacgacgc agcgcttcgg
     3541 cgtgccgctc ggctacggcg gcccgcacgc ggcctatctc tcgaccaagg atgaatacaa
     3601 gcggcagatc cccgggcgca tctgcggcat ctcgatcgac gccgagggta agccggcggt
     3661 tcgcctggcg ctgcagacgc gcgagcagca catccggcgc gagaaggcga ccagcaacat
     3721 ctgcacggcg caggtgctgc tggcggtggt ggccggcatg tacgcggtct ggcacggccc
     3781 cgaggggctg aaggagatcg cccgccgcgt ccatctgctg acggccacgc tcgacgccgg
     3841 cctcaagcgg ctcggctacg acaccggcga tcagctctac ttcgacacgc tgcgcatcga
     3901 caccggcaag acggcggccc gcgcgatcat cgaccgcgcg ctgaaccacg agatcaacct
     3961 gcgcccgatc gacgaccaca ccgtcggtat ctcgctggat caggtcacca gccaggagga
     4021 tgtcgcagag ctgctgacga tcttcaacgg cggccagccg ctcgacttcg agctgggcga
     4081 gatcgtgatg accttggaca ccagctatcc cggctcgcac gcgcgcacca gtccctacct
     4141 ggagcaccca gtcttcaaca cgcatcactc cgagaccgag atgctgcgct acatcacccg
     4201 gctggagcac cgcgatctct cgctcaatta ctcgatgatc ccgctcggct cgtgcaccat
     4261 gaagctcaac gccaccgccg agatgatccc ggtcacctgg ccggagttcg gcggcatcca
     4321 cccctacgtg cccgaggggc aaacacccgg ctaccacgag atgatgatcc ggctggagaa
     4381 gatgctggcc gagatcaccg gcttcgacga ggtcagcttg cagccgaacg ccggctcgca
     4441 gggcgagtac accggcctgc tggtgatccg ggagtatcag aagtcccggg gccaggagca
     4501 ccgcgacatc tgcctgatcc cctcctcggc gcacggcacc aacccggcca gcgccaccat
     4561 ggccggcttc aaggtggtag tggtcaagtg cgacaacgag gggaatatcg aggtcgacga
     4621 cctgcgcgcc aaggccgagc agcaccagga gaacctggcg gcgctgatgg ttacctaccc
     4681 ctcgacgcac ggcgtcttcg aggagtcgat cgtcgagatc tgcgacatcg tgcaccagca
     4741 cggcgggcag gtctacatgg acggcgccaa catgaacgct caggtcggcc tctgccgccc
     4801 ggccgagatc ggcgccgacg tctgccacct gaatctgcac aagaccttct gcatcccgca
     4861 cggtggcggt ggcccgggca tgggcccgat cggggtcaag tcgcacctgg cgcccttcct
     4921 gccgacccac ccagtggtcc acatcggtgg tccggaggga atcgggccgg tggcggcagc
     4981 gcccttcggc agcccgagca ttctgaccat ctcctacgcc tacatctcga tgatgggcgc
     5041 gcatgggctg accgaggcga ccaaggtggc gatcctcaac gccaactaca tcgccaagcg
     5101 gctcgacccg cactaccccg tgctctacaa gggcaagaac ggcatggtcg cgcacgagtg
     5161 catcgtcgac ctgcgccagc tcaaggagcg caccggcgtc gaggtcgcgg atgccgccaa
     5221 gcgcctgatg gactacggct tccacgcgcc gacgatctcc ttcccggtgc ccggcacgat
     5281 gatgatcgag ccgaccgaga gcgaggacca gggcgagatg gaccgcttct gcgacgccat
     5341 gatcgccatc cgcgacgaga tcgccgagat cgagctgggg atggccgatc gcgaggacaa
     5401 cctgctcaag aacgcgccgc acaccgcgct gatgatcgcc agcgacgagt gggcccaccc
     5461 ctactcgcgc atgaaggccg cctacccggc gcactggctg atcggcaaca agttctggcc
     5521 gccggtggcg cgtatcaacg acgtctacgg cgaccgcaac ctcgtctgcg cctgcccgcc
     5581 ggtcgagacc tacgcggact gacgcgacgc gtacccagac ggtagcaggc ggcccgcccc
     5641 ggcggcgggc cgctgccgtt tggtagaatc gtttgaacat gtcgaatagc aaccaagaac
     5701 gcagggagat ctgatggccg ggaagactgt gtacggcggc caggcggtca tggagggtgt
     5761 catgatgcgc gggcgcgaca gcatggcggt cgccgtgcgc gccccggatc agagtatcgt
     5821 cgtctaccac gagccgctgg cggcctcgcc ctgggcgctg cgggtcaaga actggccgct
     5881 ggtgcgcggc gtggtcatgc tctgggacac catggtgctg ggtacgcggg cgctcgtctt
     5941 ctcggccaac atcggtctca ccgaggaagc cgaggaggac caggagccgt cggcgctggc
     6001 ggggccggcg ctctggggca cggtcgccat ctcgatcctc ttttcgatcg gcctcttctt
     6061 cctcctgccc ttgctggtcg ttcatctgat cgaccgctac atcgacagct cggtcgtcag
     6121 caatctggtc gaggggctga ttcgcctcgg cctgctgctc ggctacctgg gcctgctggg
     6181 gaacctgcgc gatatccggc gcgtgttcgg ctaccacggc gccgagcaca agacgatcaa
     6241 cgccctggag gcgggcgacc cgctcgacgt cgagcacgtg cgccggcaca ccttgcttca
     6301 cccgcgctgc ggcaccggct tcctgctggt agtggcggtc atctcggtga tcgtcttcgc
     6361 tttcctgggc cggcccgact tcgtgctgcg ggtcctctca cgcatcctgc tggtgccggt
     6421 gatcgccgcc atcgcctatg agttcctgaa gttcaccgcc aagcatgacc gcaatccgat
     6481 cgtgcgcacg gtcatcgcgc ccagcctggc gatgcagcgc ctgacgacgc gcgagccgga
     6541 cgacgagatg ctgcaggtcg cgatcgtctc gctgcagcgc gtgctggtgg ccgagggcgc
     6601 gctctcggcg gccacgctcg cgcggccggg catccgcgag gtggacatcg atgggcacgt
     6661 ggcggggcag ccgggtgcgg cgatcgagcc gacccccgcg ctggggattg actagcgaac
     6721 cgccgaactg ggcaggcacg gaggtctgcc cgtaccggaa agcggcccgg cgggtggaca
     6781 acatcagtcg taccctc
//
LOCUS       DASUBI010000219         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1155338, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000219 DASUBI010000000
VERSION     DASUBI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155338"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFU72_04280"
     CDS             complement(<1..221)
                     /locus_tag="VFU72_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5422735.1"
                     /translation="MTLPAVLPPREATPKAGAAIIEAHGIEKTYDTGKVRVPALRGVD
                     LTVHRGEMIAIMGPSGCGKTTLLNCLSGL"
     gene            complement(754..>2283)
                     /locus_tag="VFU72_04285"
     CDS             complement(754..>2283)
                     /locus_tag="VFU72_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5422736.1"
                     /translation="VTMLAAARIGAIHIVVFAGFGSGALGERIRLAGARTLFTADITY
                     RKGQDVPLKGIVDAALAGDSPVEQVVLLRRGREDVPMTAGRDLTWDDFLQRAAGQDDG
                     YEVMEANEPAYILATSGTTAKPKLAVHSHGAYQVGIHQSGSVCFGLQPTDVWWATADI
                     GWAVGHSYILYDPLLVGCTTIAYEGAIDYPGPENPYRIISQNRVSGFFTAPTAARLLM
                     RYGTEAAEGYDLSSVERVVCAGELLNPPAWEWLQKDLFGDRVPVIDHWWQTETGGPVV
                     GNPYGINLLPIKPGSAGIPLPGIELEVIDPEGKPCGTNEKGIVVIKHPFPGLAATLWG
                     DPERYGRDYWGIVPGVYFTGDAAAIDEDGYVWFSGRADEIIKVAGHRIGTVEVETAFL
                     RHPAVAEAGVTGRPDELRGEVISCFVVLKPGQEQSKSLKDELQQTVRSELGPLAVISD
                     INFVSVLPKTRSGKIMRRVLKAVTLDRDPGDISTIEDEGSVDEARAAWQAMKAEVSGG
                     S"
BASE COUNT          347 a          762 c          792 g          382 t
ORIGIN      
        1 tcgagtccgg agaggcagtt gagcagggtg gtcttgccgc agccggaggg acccatgatc
       61 gcgatcatct cgccacggtg caccgtcaga tcgacgccgc gcagcgccgg cacccgcacc
      121 ttgccggtgt cgtaggtctt ctcgatgccg tgcgcctcaa tgatggcggc ccccgctttg
      181 ggggtggcct cgcgcggcgg gagtacggct gggagcgtca tactggatgt gtccatcggg
      241 cacctcgcgt ctatttcttc gatgccggcc atgcgagccc ggacggccta gggctggagc
      301 agggctgcag ccccaagcct atggtgatgg gcgtcacgaa ggagtaacgg gagcgcacgg
      361 ggtgtgacaa aaccgtagcg aacgaggcag catccagtga ggtgccccgt tgcggggcgc
      421 aagggcagga cgccggccac tgcccgagcc cgatcgaagc ggtcgcctcg gagttgtctg
      481 gcgaagacat tggctgccgc gtggggcacg aatccttcga ctgtgctcgg gatgaccggg
      541 ggaggggatg cggcctagcg gacgaggcgt atcatcgtcc gacgctcatc ctcgtcagcc
      601 cggccttaac cctgtcattc cgaccgaagt ggagggatcc gtcctcgccc ggcacccttg
      661 cccctggcca gccaccggct cacatgcgcg caaaaccagg ccaggagggc gatccggccg
      721 cgcacggcag tcgttcgagt cgtcagacag tgcctagcta ccgccagaga cttccgcctt
      781 catggcctgc caggcggcgc gagcttcgtc gaccgagcct tcgtcctcga tggtcgagat
      841 gtcgcccggg tcgcggtcga gcgtgaccgc tttgagcacg cggcgcatga tcttgccgct
      901 gcgcgtcttg ggcagcacgc tgacgaagtt gatgtcgctg atgaccgcca gcggccccag
      961 ctcgctgcgc accgtctgct gcaattcgtc tttgagggac ttgctctgct cctggcccgg
     1021 cttgagcacc acgaagcagg agatgacctc gccgcgcagc tcgtcggggc ggccggtgac
     1081 gcccgcctcg gccaccgccg gatgacgcag gaacgcggtc tcgacttcga ccgtgccgat
     1141 gcggtggccg gccactttga tgatctcgtc ggcccgcccg ctgaaccaaa cgtagccgtc
     1201 ttcatcgatc gcggcggcgt cgccggtgaa atagacgccg ggcacgatgc cccagtagtc
     1261 acgcccgtag cgctccgggt cgccccagag ggtggcggcc agcccgggaa acgggtgctt
     1321 gatgaccacg attcccttct cgttggtgcc gcaaggcttt ccctccgggt cgatcacctc
     1381 cagctcgatc cccggcagcg ggatgccggc cgagccgggc ttgatcggca gcaggttgat
     1441 gccgtacggg ttgccgacga ccggcccgcc ggtctcggtc tgccaccagt ggtcgatcac
     1501 cggcacgcgg tcgccgaaca agtccttctg tagccactcc caggcgggcg ggttgagtag
     1561 ctcgccggcg cagaccacgc gctcgaccga gctgagatca tagccctcgg cggcctcggt
     1621 gccgtagcgc atcagcagcc gggcggccgt tggcgcggtg aagaaaccgc tgacccggtt
     1681 ctggctgatg atgcggtagg ggttctcggg gccggggtag tcaatcgcgc cctcgtaggc
     1741 gatggtggtg cagccgacca gcagcggatc gtagaggatg tagctgtgcc ccaccgccca
     1801 gccgatgtcg gccgtagccc accagacgtc ggtcggctgc aggccgaagc agacgctgcc
     1861 ggattggtgg atgccaacct ggtaggcgcc gtggctgtga acggccagct tcggcttggc
     1921 ggtcgtcccc gaggtcgcca ggatgtaggc cggctcgttg gcctccatga cttcgtagcc
     1981 gtcgtcctgc cccgccgcgc gctgcaggaa atcgtcccag gtcaggtcgc gccccgccgt
     2041 catcggcacg tcttcacggc cacgtcgcag cagcacgacc tgctcgacgg gcgagtcgcc
     2101 ggccagcgcg gcgtcgacga tccctttgag cggcacgtcc tgacccttgc ggtaggtgat
     2161 gtcggcagtg aagagggtgc gcgcgccggc cagcctgatg cgctctccca gcgcgccgct
     2221 gccgaagccg gcgaagacga cgatgtggat ggcgccgatg cgggctgccg ccagcatggt
     2281 gac
//
LOCUS       DASUBI010000220         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_795818, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000220 DASUBI010000000
VERSION     DASUBI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795818"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFU72_04290"
     CDS             complement(<1..713)
                     /locus_tag="VFU72_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5422737.1"
                     /translation="MIARRLLAPPPGWIRRADVVVVGSGIAGVTAALTAHASLPDSNV
                     LLVTKSMLDDGSTRWAQGGIAAAIGPGDSPEQHLADTLTAGAGLCDVNAVRTLVTDGP
                     DAVRRLIALGARFDSEPGGPLALTREGGHLRRRIAHAGGDATGAEISRALLVAVKEAD
                     EGTGGGLDVIEHAMSLDLLLTPDGRAAGLTLHVIGEGQQDGVGAVSAPVVILATGGIG
                     QIYSATTNPQVSTGDGVALA"
     gene            complement(710..1672)
                     /gene="panC"
                     /locus_tag="VFU72_04295"
     CDS             complement(710..1672)
                     /gene="panC"
                     /locus_tag="VFU72_04295"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5422738.1"
                     /translation="MTLSDCSACFPGRHHDRYSMIPVVARTMAGMTAARATMRSPVVL
                     VPTMGALHDGHRALLRHARRMAGQDGSVVVSVFVNPLQFGAGMDLDRYPRTLASDVSL
                     CAEEDVAVIFAPLVDQMYPFKQEITVNPGSVGEILEGAFRPGFFTGVLTVVLKIFNLV
                     RPDAAVFGQKDAQQVFLVRRMVADLNLLVDISSVATVREPDGLAASSRNSYLSPGERL
                     TALALSRALNAGAAAGAGGQQAVLTAARAELDAAAEADPPLLTDYLELVDPATFATVD
                     SGYAGAAQLLVAGTVGKTRLIDNALVIVGPVGSAEPSGPAGSTP"
     gene            complement(1626..>2000)
                     /locus_tag="VFU72_04300"
     CDS             complement(1626..>2000)
                     /locus_tag="VFU72_04300"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEU5422739.1"
                     /translation="EDKRGIYHAALARAANHLVTLVVEATDMLNAAGVADPARMLGPL
                     LAASLENSLALGPAALTGPVARGDAETVAAHIAAIKATAPEALAAYLALARLTADRAL
                     AAHRLTADDAERLLGVLSGPPS"
BASE COUNT          314 a          722 c          674 g          290 t
ORIGIN      
        1 gcgagcgcga ccccgtcgcc ggtggagacc tgcgggttgg tcgtggccga gtagatttgg
       61 ccgataccgc cggtcgccag gatcacgacc ggcgcggaca cggcgccaac cccgtcctgc
      121 tgcccttcgc cgatcacgtg cagcgtcagg ccggcggcgc ggccgtcggg cgtgagcagc
      181 aggtcgagcg acatcgcgtg ctcgatcacg tcgaggccgc cgccggtgcc ctcgtcagcc
      241 tccttcaccg cgacaagcag cgcacgcgag atctcggcgc cggtggcgtc accgcccgcg
      301 tgcgcgatcc tgcggcgcag gtggccgccc tccctggtga gagcgagcgg accgccaggc
      361 tcggaatcaa accgggcgcc aagcgcgatc agccgcctga ccgcgtcggg cccgtcggtg
      421 accagcgtcc tgaccgcgtt gacgtcgcag agcccggctc ccgccgtcag cgtgtcggcc
      481 aggtgctgct cgggcgagtc gcctggcccg attgcggcgg ctatgccgcc ctgcgcccac
      541 ctagtcgagc cgtcgtcgag catggacttg gtcaccagca gcacgttgct gtcaggtagc
      601 gacgcgtgcg cggtcagcgc cgccgtcacc ccggcgatgc cggatccgac caccaccacg
      661 tcggcgcgcc tgatccagcc gggcggcggc gcgagcaacc gccgcgcgat cacggcgtgc
      721 tccccgcggg cccggacggc tccgccgacc ctacgggccc cacgatcacc agggcgttgt
      781 cgatcagcct ggtcttgccc acggtgccag cgaccaggag ctgcgcggcc ccggcatagc
      841 cggaatccac ggtggcgaac gtcgccggat cgaccagctc cagatagtcg gtcagcagcg
      901 gcgggtcggc ctcggcggcg gcgtcgagtt cggcccgggc tgccgtcagc acggcttgct
      961 ggccgccggc accagcggcc gcgcccgcgt tcagtgccct gctcagcgct agtgccgtca
     1021 gccgctctcc gggcgacagg taactgtttc tgctcgaggc agcgaggccg tcgggctcgc
     1081 gcaccgtagc gaccgacgag atgtcgacca acaggttcaa atccgccacc atccgccgca
     1141 cgaggaacac ctgctgagcg tccttctgac cgaatacagc cgcatccggc ctgaccaggt
     1201 tgaatatctt cagtaccaca gtcaggacgc cggtgaagaa ccccggcctg aacgctcctt
     1261 cgagaatctc gccgaccgat cccggattta ccgtaatttc ttgcttaaaa gggtacatct
     1321 ggtcgacgag aggggcgaag atcactgcga cgtcttcctc agcacagagg ctcacgtcgc
     1381 tggcgagcgt gcgcggatac ctgtcaagat ccattccggc gccgaactgg agcgggttca
     1441 cgaagacaga caccacgaca gaaccgtcct gaccagccat ccgccgagcg tgccgcagca
     1501 gcgcgcgatg cccgtcatgc agcgccccca tggtcggcac gagcacgaca ggagagcgca
     1561 tggtcgccct ggccgcggtc atccctgcca tggtcctggc taccaccgga atcatgctgt
     1621 accgatcatg atggcggccc ggaaagcacg ccgagcagtc gctcagcgtc atcggcggtc
     1681 agcctgtgcg ccgccagcgc acgatcggcg gtcagccggg cgagcgccag gtaagcggcg
     1741 agggcttcgg gggctgtggc cttgatcgcc gcgatgtggg ccgccacggt ttccgcgtcg
     1801 cctcgagcga ccgggccggt gagcgcggcc ggaccaagcg ccagcgagtt ctcgagcgac
     1861 gcggccagca atgggccgag catcctggcc ggatcagcaa cgccggccgc gttcagcatg
     1921 tccgtcgcct ccacgacgag cgtgaccagg tggttggccg ccctggcgag cgccgcgtgg
     1981 tagatgccgc gcttgtcctc
//
LOCUS       DASUBI010000221         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_661213, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000221 DASUBI010000000
VERSION     DASUBI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661213"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFU72_04305"
     CDS             <1..193
                     /locus_tag="VFU72_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5422740.1"
                     /translation="WVDRLLAAADNPEAARRALEDRHPQRRLGTPEQVAAAALYLASD
                     AAAFVTGSALVMDGGQSAR"
     gene            272..1372
                     /gene="ugpC"
                     /locus_tag="VFU72_04310"
     CDS             272..1372
                     /gene="ugpC"
                     /locus_tag="VFU72_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5422741.1"
                     /translation="MAQVIYDHVTKRFDGVVALNDLELDITDGEFLVLVGPSGCGKST
                     ALRCLAGLEEITSGNIMIGDRVVTNIPPKDRDIAMVFQAYALYPHMSVRDNLAFGLKL
                     RHTPKDEIERRVRQAADMLGLEALLDRKPRALSGGQRQRVALGRAIVREPQVFLMDEP
                     LSNLDAKLRVQTRAEIIKLQERLGTTTVYVTHDQVEAMTMGDRIAVMSAGLLQQLDTP
                     QNLYDHPANLFVATFIGSPAMNIFPAEVVRGDGGLHLKTDGIDLLLPGIREGKLGSYV
                     GQQVMIGIRPEHLSVAEGSVSPERSMRLPVELVEPLGAETLVHFAGPDGTIIVARVQP
                     ETRVKEGQMLDLVVETEHLHAFDRDSEQSLIS"
     gene            complement(1392..2648)
                     /locus_tag="VFU72_04315"
     CDS             complement(1392..2648)
                     /locus_tag="VFU72_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422742.1"
                     /translation="MSTLSQRAARVRIQLSRFPGTLWLLAGANLVLFTARGMTIPFLV
                     IYFGQILGLGEGHVGAGIAASSIAGVLFTLAAAGTIDRFGPRNMVVITTLGTAATTAL
                     FPVATDLTRFFALMVVQGLFTQLYWPSSDTLATTLVPVEQAGEMFALLRVASALGTGA
                     GGLIGGLMVAGGTIPEFQLLYWVAGGGIGLAGILVLALVCQPRRQRLAEHQQAGGSWR
                     EVLTDRRFMFSQIVMFILLAGLTQFQVTAPPYLHAQAGLGETAVGSLFTINTIIVVAA
                     QLLVANRIARWGRGISLSAAALFWCVSYAMIGASPWVAGLPFAAAVVYTLGEMIFMPT
                     SGVITVELAPERLRGRYLAFSSVIWGSAWGLASWAAGIALGSAHPTLLWPALIVLLLI
                     GAAGGWLFDRTAPRVVAPVAVTTQDD"
     gene            3071..>3644
                     /locus_tag="VFU72_04320"
     CDS             3071..>3644
                     /locus_tag="VFU72_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5422743.1"
                     /translation="MSAISGFAEAAASVTSLDELTLALRATVTTLLPAETASLEIWDE
                     ALAAAPANDRARAFQTAFASSLSPVRAPRDNGTAQLAVPVCVGRVPIGALVVESERAG
                     EVGEHDESMLHDLAREAARAVVRLATRPASSPAHQNGQHDAPSNAQSATPSGRFLQAL
                     LEGEEIQQILNDAAEAAARRVGADGAEIRLS"
BASE COUNT          619 a         1251 c         1276 g          498 t
ORIGIN      
        1 gtgggtcgac cggctgctgg cggcggcgga taaccccgag gccgcgcggc gcgcgttgga
       61 ggaccgccac ccgcagcgcc gcctcggcac gccggagcaa gtggcggccg cggcgctcta
      121 tctcgcgtcc gacgcggcgg ccttcgtcac gggcagcgcg ctggtgatgg acggcggcca
      181 gtccgcgcgc taggcacggg tagcgtacga tcgggggtat tccggagagc agacggacgg
      241 tggcagtcgt cgttgcggaa gggacatggt catggcccag gtgatttacg atcacgtgac
      301 caagcggttc gacggcgtcg tggcgctcaa tgacctggaa ctcgatatca ccgacggcga
      361 gttcctggtg ctggtcggcc cctccggctg tggcaagtcg accgcgctgc gctgcctggc
      421 cgggctggag gagatcacct ccggcaacat catgatcggc gaccgggtcg tgaccaacat
      481 cccgcccaag gaccgtgaca tcgccatggt cttccaggcg tacgcgctct atccccacat
      541 gtcggtgcgc gacaacctgg ccttcgggct caagctgcgc cacacgccca aggacgagat
      601 cgagcgccgg gtgcgccagg cggccgacat gctcggactg gaggcgttgc tcgaccgcaa
      661 gccgcgcgcg ctctccggcg gccagaggca acgcgtggcg ttgggacgcg ccatcgtgcg
      721 cgagccgcag gtcttcctga tggacgaacc gctctccaac ctcgacgcca aactgcgcgt
      781 gcagacgcgc gccgagatca tcaagctaca ggagcgcctg ggcaccacca ccgtctacgt
      841 cacccacgac caggtggagg cgatgacgat gggcgaccgc atcgcggtca tgagcgccgg
      901 tctcctgcaa caactcgaca ccccgcagaa cctctacgac cacccggcca acctcttcgt
      961 ggccaccttc atcggcagcc cggcgatgaa tatcttcccg gccgaggtgg tgcgcggcga
     1021 cggcgggctg cacctgaaga cggacggcat cgacctgctg ctaccgggca tccgtgaggg
     1081 caagctgggg agctacgtcg gccagcaggt aatgattggc atccggccgg agcacctgag
     1141 cgtggccgaa gggagtgttt cgccggagcg ctcgatgcgc ctgccggtgg agctggtcga
     1201 accgctgggg gcggagacgc tggtccactt cgccgggccg gatggcacga tcatcgtggc
     1261 gcgggtgcag ccggagacgc gcgtcaagga ggggcagatg ctcgacctgg tagtcgagac
     1321 cgagcacctg cacgcctttg accgtgacag cgagcagtcg ctgatctcgt aggccataag
     1381 ccggaaccgg ctcaatcgtc ctgggtggtg acggcgaccg gcgcgaccac gcgcggcgct
     1441 gtgcgatcga agagccagcc gccggccgcg ccgatcagca gcagcacgat gagcgccggc
     1501 cagaggagcg tggggtgggc ggagccgagc gcgatgcccg ccgcccatga cgccaggccc
     1561 caggcgctgc cccagatcac gctgctgaag gccaggtagc gcccccgcag ccgctccggc
     1621 gccagttcca ccgtgatcac gccggaagtc ggcatgaaga tcatctcgcc cagcgtgtag
     1681 accaccgccg cggcgaacgg gagcccggct acccacggac tggcgccgat catggcgtac
     1741 gacacgcacc agaagagggc ggcggcggac agcgagatgc cgcgccccca gcgcgcgatg
     1801 cggttggcca ccaacaactg cgccgcgacc acgatgatcg tgttgatcgt gaagagcgag
     1861 ccgacggccg tctcgcccag cccggcctgg gcgtgcaggt agggcggcgc ggtcacctgg
     1921 aactgcgtca gccccgccag caggatgaac atcacgatct gcgagaacat gaagcggcgg
     1981 tcggtcagta cctcgcgcca gcttccgccc gcctgctgat gctccgccag tcgttgccgc
     2041 cgcggttgac ataccaacgc cagcacgagg ataccggcca gcccgatacc gccacccgcg
     2101 acccagtaga gcagctgaaa ctcggggatg gtgccgcccg cgaccatcag gccgccgatc
     2161 agcccgccag cgccggtgcc gagcgcgctg gcgacccgca gcagcgcgaa catctcgccc
     2221 gcctgttcga ccggcaccag ggtcgtcgcc agcgtgtcgg aggagggcca gtagagctgg
     2281 gtgaaaagcc cctgtaccac catgagggcg aagaagcggg tgagatcggt ggcgacgggg
     2341 aagagcgcgg tcgtggccgc cgtccccagg gtggtgatca cgaccatgtt gcgcggcccg
     2401 aagcggtcga tggtgccggc cgctgccagc gtgaagagca ctcccgcgat cgagctggcc
     2461 gcgatgcccg cgccgacgtg cccctcgccc agaccgagga tctggccgaa gtagatcacc
     2521 agaaagggga tggtcatacc ccgcgcggtg aagagcacca gattggcccc ggccagcaac
     2581 cagagggtgc cggggaagcg cgacagttgt atccgaacgc gcgcggcccg ctgcgaaagc
     2641 gtgctcacct ctccccttct cgtcgggggc aagtgtggca ggggcgactc gcccgcgcca
     2701 ctggccgagc ggccagctat ccgaagggat agcggtaccg gagagaaccg ctgggaagcc
     2761 caggggcgca gacatgcgcc gggctgcgct gccccgcgcg gcgcccgaac caacgcgagc
     2821 cagcgtgcgt tcacgcgccg ccggtcggtt ttgcctggag tgcggaccaa tggcgcaggt
     2881 ggaacggccg tgttccaacc ttcttcctat gttccacggc cgcaaatcac gcgtatcgtg
     2941 ggcgatatgg agttctggcg tcgtcgtttc gactcttggt gggcggcgcc ggtcccagcc
     3001 ctaacgggaa ccggcgagat gacaagggga tagcaggctg atgggttcgt cgctgcgggt
     3061 tgacggggca gtgagcgcga tctctggctt tgccgaggca gcggcctccg tcaccagcct
     3121 ggacgagctc actctggcgc tgcgcgccac cgtcacaacg ctgctcccgg ccgaaaccgc
     3181 cagcctggag atctgggacg aggcgctggc tgccgcgccg gccaacgacc gggcccgcgc
     3241 cttccaaacc gccttcgcca gctcgttgtc gccggtacgt gcgccccgcg acaacggcac
     3301 ggcccagctc gcggtgccgg tctgtgtcgg ccgggtgccg atcggcgcgc tggtggtgga
     3361 gtccgagcgc gcgggcgagg tcggcgaaca cgacgagtcg atgctgcacg atctcgcgcg
     3421 cgaggccgcg cgtgccgtcg tccgcctggc gacgcgtcca gccagcagcc ctgctcacca
     3481 gaacggccag catgacgcgc cgagcaacgc gcaatccgcg acgccctccg gccgtttcct
     3541 gcaggcgctc ctcgaaggtg aagagatcca acagatcctc aacgacgccg ccgaggccgc
     3601 tgcgcgccgc gtcggcgccg acggagccga gattcgtctc agcg
//
LOCUS       DASUBI010000222         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_167617, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000222 DASUBI010000000
VERSION     DASUBI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167617"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFU72_04325"
     CDS             complement(<1..741)
                     /locus_tag="VFU72_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5422744.1"
                     /translation="MSETTRARDLGVRLGRLAPGRWNAITDVAGVRVGHTTLIEGEGP
                     LVVGQGPVRTGVTVIQPHKGNAGADPVFAGYHVLNGNGEMTGLAWLAEAGLLMTPVAI
                     TNTHSVGVVRDALIAYDVAHGGKQEYSWSLPVVAETYDGFLSDIDGQHVRPEHVFAAL
                     DAASAGPVPEGNVGGGTGMICHGFKGGIGTASRVLAEADGGWTVGALVQANYGQRALL
                     RIGGVPVGQAIGPEVVPLPGVRPEGAGSI"
     gene            complement(872..1615)
                     /locus_tag="VFU72_04330"
     CDS             complement(872..1615)
                     /locus_tag="VFU72_04330"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEU5422745.1"
                     /translation="MAAVETPDIQRPPKELVEGLAQIGSATAAGELSRLGIRNPHMRG
                     ITSWTPGKAVAGPALTLQFMPKREDIYRVDEYSDPEKQLHRHVLYHTQPGDVVVVAAR
                     GDLSSGVFGDMMLTYFKGRGGAGVVIDGCIRDYPKAQRLGLGLWLRGVTPNFHTQTEI
                     YPYAVNVPIACSNVLVMPGDIIVADDDGAVVVPITLAPQLLAKASEHAEWEDFSRQRL
                     MEGGDLRRYYPLSDAARPEYEEWKRKQGK"
     gene            complement(1765..2487)
                     /locus_tag="VFU72_04335"
     CDS             complement(1765..2487)
                     /locus_tag="VFU72_04335"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEU5422746.1"
                     /translation="MTNRDSSSRTVGILGGMGPLATVDLYRKIILATPATCDQEHLHV
                     IIDADPTVPDRTAALRGDAPDPLPWLARGARRLETAGANFIVIPCNTAHAWLPRLRPL
                     VGIPILDMIAEAVARVRADYPDARRVGLLATRGTIGSALYHQALTAQNLTALVPAPDG
                     EQHLVDRAIGAVKAGDTGRAASDPLVTAARALAAAGAEVLLAACTEIPLALTAAQAPV
                     PLLDPTQVLAEAAVREATQQPA"
     gene            complement(2491..3591)
                     /locus_tag="VFU72_04340"
     CDS             complement(2491..3591)
                     /locus_tag="VFU72_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5422747.1"
                     /translation="MSAEQRCRVRDLGVVIGELPPGPLNAITDVAGVRVGHATIVRGE
                     GPLVVGKGPVRTGVTAILPHGGDTFAEKVPAAAEVFNGFGKSIGLVQIAELGQLETPI
                     LLTNTLSVGRVADALVGYMIERHPAIGVTTGTVNPVVCECNDGWLNDIGGRHVGETEV
                     RAALASATADAVPEGNLGAGAGMTCYGFAGGIGTASRRLDAAHGGYTVGALVLANFGR
                     RADLTIAGVPAGRALARRDPAPAPREQGSVIVALATDAALDARQLGRLCRRVPLGLAR
                     TGTHGGHGSGDLAIAFSTADRIPHTATPLTRTITVLNEQSAALDLLFRAVVETTEEAV
                     INALCMARPLTGRDGHHREALPLDVVRMTNDE"
BASE COUNT          551 a         1386 c         1240 g          584 t
ORIGIN      
        1 gatcgagccg gcgccctccg gccggacgcc aggcagcggc acaacctccg gcccgatggc
       61 ctgcccgacc ggcacgccgc caattcgcag cagcgcccgc tgcccgtagt tcgcctgcac
      121 cagcgcgccg accgtccagc caccatccgc ctcggccagc acgcgcgagg ccgtgccgat
      181 gccgcccttg aagccgtggc agatcatccc cgtgccgccg ccgacgttgc cctccggcac
      241 cggaccggcg ctggcggcgt cgagcgccgc gaagacgtgc tcgggccgga catgttggcc
      301 gtcaatatcc gacagaaagc cgtcgtaggt ctcggcgacg accggcaggc tccacgaata
      361 ttcctgcttg ccgccgtgcg ccacgtcata ggcgatcagc gcgtcgcgca ccacgccgac
      421 actgtgcgtg ttggtgatgg cgaccggcgt catcagcaga ccggcctccg ccagccaggc
      481 caggccggtc atctcgccgt taccgttcag gacgtggtag ccggcgaaga cgggatcggc
      541 cccggcgttc cccttgtgcg gctgaatgac agtcacgccc gtccgcaccg gcccctgtcc
      601 gacgaccagc ggcccctcac cctcgatcag cgtcgtgtgg ccgacgcgca cgccggccac
      661 gtcggtaatc gcattccagc gtcccggcgc cagccgcccg agccggacac ccaggtcgcg
      721 cgcccgcgtc gtctcgctca tttgcccttg cctggtgatg gcccccgccc ccaattctgg
      781 gggagcaccc atccgaacgc cggggcatgt ctgcgaaccg cccaggctaa ccagcgtgct
      841 cccccaggat tggggggcgg gggccgttcc tctatttccc ctgcttgcgc ttccactcct
      901 cgtattcggg gcgggcggca tcggagagcg gatagtaccg gcgcaggtcg ccaccttcca
      961 tcaggcgctg gcgcgagaaa tcctcccact cggcgtgctc gctcgccttc gccagtaact
     1021 gcggcgccag cgtgatcggc accaccaccg cgccgtcatc atcggcgacg atgatgtcgc
     1081 cgggcatcac cagcacgttg ctgcacgcga tcggcacgtt gaccgcgtag ggatagattt
     1141 cggtctgcgt gtggaagttg ggcgtcacgc cgcgcagcca tagccccagc cccagccgct
     1201 gcgccttcgg gtagtcgcgg atgcagccgt caatgacgac gcctgcgcct ccacgcccct
     1261 tgaagtaggt gagcatcatg tccccgaaga cgccgctgct caggtcgccg cgcgcggcta
     1321 cgaccacgac atcgcccggc tgcgtgtggt agagaacgtg ccggtgcaac tgcttctccg
     1381 gatcggaata ctcgtccacc cggtagatgt cctcgcgctt gggcatgaac tgcaaggtga
     1441 gggccggacc agccaccgcc ttgcccggcg tccagctcgt gatgccgcgc atgtgcgggt
     1501 tgcgaatgcc cagccggctc agttcgccgg ccgccgtcgc gctgccgatc tgtgccagtc
     1561 cctcaaccag ttccttcggt ggacgctgaa tatccggtgt ttcgaccgcc gccatcgctt
     1621 tcccctcctc tggcccgggc aactctgccc cggtcacctg ccaacgacac cagccgcccc
     1681 agccgggacc gcgccaatgc ccacaccgag cggtgcccgc cgagtatagc agccagtaga
     1741 cactgaatgg tggttaagcg atagtcaggc aggctgttgc gtcgcctcgc gcaccgccgc
     1801 ctccgccagc acctgcgtgg ggtcgagcag cggcacgggt gcctgcgcgg cggtcagcgc
     1861 cagcggaatc tccgtgcagg cggccagcaa cacctccgcg ccggcagcgg cgagcgcccg
     1921 cgccgccgtg accagtgggt cgctggcggc tcggccggta tcgcctgctt tgaccgcccc
     1981 gatcgcgcgg tccacgaggt gttgttcgcc atccggcgcg gggacgagcg cggtcaggtt
     2041 ttgcgcggtc agcgcctggt gatagagcgc gctgccgatg gtgccgcgcg tcgccagtag
     2101 tcccacgcgg cgcgcgtcgg ggtagtcggc acgcacgcgc gccaccgcct cggcgatcat
     2161 gtcgaggatc gggatgccga cgaggggccg caggcgcggc agccaggcgt gcgccgtatt
     2221 gcaggggatg acgatgaagt tcgcgccggc cgtctccagt cgccgcgccc cgcgcgccag
     2281 ccagggcagc gggtcgggcg cgtcgccgcg tagcgcggcc gtgcggtccg gcacggtcgg
     2341 gtcggcgtca atgatgacgt gcaggtgttc ctggtcgcac gtcgccggcg tcgcgaggat
     2401 aatcttccgg tagaggtcga cggtcgccag cggccccatc ccacccagaa tcccgaccgt
     2461 ccggctgctg ctatcacgat tcgtcattcg tcactcgtca ttcgtcattc gcacaacgtc
     2521 cagcggtagc gcctcgcgat gatgcccgtc gcggccagtc aatggccgag ccatgcagag
     2581 cgcattgatg accgcctcct cggtcgtttc gaccaccgcg cgaaaaagca gatcgagcgc
     2641 cgcgctctgt tcgttgagga cagtgatggt ccgcgtcaac ggcgtggcgg tgtgcgggat
     2701 gcggtcggcg gtcgagaagg cgatggcgag gtcaccgctg ccatgcccgc cgtgcgtgcc
     2761 ggtgcgcgcc agcccgagcg gcacgcgacg gcagaggcga ccgagctggc gcgcgtcaag
     2821 cgccgcgtcg gtcgccagcg ccacaatcac cgaaccttgc tcgcggggcg ccggcgcggg
     2881 atcgcgccgc gccagcgcgc ggccagccgg cacgccggcg atcgtcaggt cggcgcggcg
     2941 gccgaagttc gccagcacaa gcgcgccgac cgtgtagccg ccgtgcgccg cgtccaggcg
     3001 acgcgaggcc gtgccgatac cacccgcgaa gccgtagcaa gtcatgcccg cgcccgcgcc
     3061 aagattgcct tcgggcaccg cgtcggccgt ggcgctcgcc agcgccgcgc gcacctccgt
     3121 ttcaccaaca tgccggccgc cgatgtcgtt cagccagcca tcgttgcatt cgcagacgac
     3181 cggattgacc gtgccggtcg tcacgccgat cgctggatgc cgttcaatca tgtaccctac
     3241 cagcgcgtcg gccactcgcc cgacactgag ggtattggtc agcaggatcg gcgtctccag
     3301 ctgccccaat tcagcaattt ggaccaaccc gatggacttg ccgaagccat tgaagacctc
     3361 ggcagctgcc gggactttct ccgcgaaggt atcgccgccg tgcggcagaa ttgccgtgac
     3421 gccggtgcgg accggtccct tcccgacgac gagcggcccc tcgccgcgca cgatcgtcgc
     3481 gtggccgacc cgcacgccgg ctacgtcggt gatcgcgttc agcggaccgg gcggcagttc
     3541 gccaatcacc acgcccaggt cgcgcacccg gcagcgttgc tccgcgctca tacggcgtag
     3601 gggctgtgcg cgcgatagct ccgctcaaac tcctcgcgca cacagagcgt gcgcgggtcg
     3661 gtcagccgat ccacggccag cacgccgtcg aggtggtcga gttcgtgctg gaggagttcc
     3721 gccaggtcgc cctcggcgtc gagcgtttgc cacgcgctgc t
//
LOCUS       DASUBI010000223         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_930702, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000223 DASUBI010000000
VERSION     DASUBI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930702"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(521..1171)
                     /locus_tag="VFU72_04345"
     CDS             complement(521..1171)
                     /locus_tag="VFU72_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5422748.1"
                     /translation="MADQTGAAQTSGAARELELAELLRGRRSVRRLRPDPVPRPLVEQ
                     VLEAGRWAPSPHGTQPWRFAVLTEPAARTRLADAMAESWQHNLAMDGQDTATIARRLE
                     GSRRRLLEAPVLIMVSLYLDDLDRYPDPERQAAETTMAIQSLGACVQNMLLMAYRLGL
                     DAGWMCAPLFCPEVAREALGLPASHLPHALLPLGYAAADPKRRPRRPLESLITVWE"
     gene            complement(1290..2477)
                     /locus_tag="VFU72_04350"
     CDS             complement(1290..2477)
                     /locus_tag="VFU72_04350"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU5422749.1"
                     /translation="MAQTRPNMRFLAQEIDELKAKGLYHPLRVVGTANEAVFTVDGQR
                     VINLSSNNYLGLTTHPKLIEAAVAATREFGVGSGAVRTIAGTMGIHQELEERLAAFKG
                     TEASLTFQSGFTANVGVLQSVLHEEDVVISDQLNHASIIDGIRLTRAQRKIFPHKDMD
                     GLEQALKETQSARVRLVVTDGVFSMDGDIAPLPQIVDLAERYNAVVMVDDAHASGVLG
                     KNGRGSVSHFGLEGRVQIQVGTLSKAIGSVGGYVAGEQVLRDFLIHMARPFLFSTSQP
                     PSVAATCIAAIDVLEQEPQLIDRLWQNARFLKEQLQQLGFNTGRSETPITPIIVGDAD
                     TAMNLSNTLFERGVFAQGIGFPTVPHGQARLRTIVSAAHTQEQLQQAVDIIARTGKEL
                     GVI"
     gene            complement(2506..3591)
                     /gene="tdh"
                     /locus_tag="VFU72_04355"
     CDS             complement(2506..3591)
                     /gene="tdh"
                     /locus_tag="VFU72_04355"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5422750.1"
                     /translation="MTETMLGIVKPERAPGAELREVPVPRPGDHDALIRVIATSMCGT
                     DLHIYRWDPWAEEHFGPTPMVFGHEYCGEVVEVGRDVTHVQVGDFVSGETHIGCGVCD
                     RCREGQAHLCERMGLVGVTRPGCFAEYICIPAENAWVNPPDLPVDTAAIQEPFGNAVH
                     TVLSGEVTARSALVMGCGPIGLFAVGIARAGGAAPIYAVDVNDFRLELALKMGATRVI
                     NGREEDPVAVIRRELGSGVDVAFEMSGSPIGTRQAFAALRPGGRISMLGLADEPFEFD
                     FSNLLVLKGIEVHGIFGRKMFETWRQTSTLLGRGLVDVAPIITHRLPLTEFQRGFELL
                     LHSQAGKVILYPTEPPEPTENHAHGAR"
     gene            4062..>4265
                     /locus_tag="VFU72_04360"
     CDS             4062..>4265
                     /locus_tag="VFU72_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422751.1"
                     /translation="MRGRAVIRRLLLALILLAPGTPAIVAQDVAAHAATQPQTVSVAA
                     LSAASAPPDDRLHLAALPTPSGAS"
BASE COUNT          618 a         1472 c         1460 g          715 t
ORIGIN      
        1 ttccgctatc agttgccgac gatctacgac agcctctacg tggcgctggc tgtggccctt
       61 gatctaccac tttggacggc cgatcgacgc cttcgtaaca gccttggcac tgcctttccg
      121 aggttacgcg tgctcgcata ggcacgaatg agcagacgcg tggcgccgcg ctttcccatg
      181 gcaaattacc gcggattcgc aaccgtttca tctagctgag cggccgtttc ctggcctgga
      241 gccgaaggaa gaggtggcgc gcatcggaag ccgggcgcgt cattgttggg ggtggagagg
      301 ggccagacac agcccggcga ccccacggtc ggcggggtcg ccgggcgtgt gactgttgcg
      361 gctcatcaga ctagagcagc cagatgagca ggatgatgat gatgatcaga gcgagaagac
      421 cgccaccgat atacatccgg tacctccgtt gccttatcca cgactgccct tcgcagtcac
      481 tctcgttaag gcaagccacg tgccacgtgg tcggcggcgg ctattcccag acggtgatca
      541 ggctctccag cgggcggcgc gggcggcgtt tggggtcggc ggcagcgtag ccgagcggca
      601 gcagcgcgtg gggcaggtga ctggcgggca ggccgagcgc ctcgcgcgcc acctcggggc
      661 agaagagggg cgcgcacatc cagccggcgt cgagtcccag gcggtaggcc atcagcagca
      721 tgttctgcac gcaggcgccc aggctctgga tcgccatggt cgtctcggcc gcctggcgtt
      781 cgggatcggg gtagcggtcg agatcgtcca ggtagagcga gaccatgatc aggacgggcg
      841 cctccagcag ccggcggcgg gagccctcca gccgcctggc gatcgtcgcg gtatcctgcc
      901 catccatggc cagattgtgc tgccagctct cggccatcgc gtcggccagc cgggtgcgcg
      961 ccgccggctc ggtcagcacg gcgaagcgcc agggctgggt tccatgcggc gagggcgccc
     1021 agcggccggc ctccagcacc tgctccacca gggggcgcgg caccgggtcg gggcgcagcc
     1081 gccgcaccga gcggcgcccg cgcagcagct ccgccagctc gagctcgcgt gccgcgccgc
     1141 tcgtttgcgc cgctccggtc tgatccgcca cgcgtccacc ttctccccta cgccggtcca
     1201 cgccgcgaag attacacggg cgcgcgggtt cgggcgaggt ggctgctccg tcgcggtggt
     1261 gggtggcgta ttcgacggag cgcgcggcct cagatcacgc ccagctcctt gccggtgcgc
     1321 gcgatgatgt cgaccgcctg ctgcaactgc tcctgggtgt gggccgcgct gacgatggtg
     1381 cgcagccgcg cctggccatg cggcaccgtc gggaagccga tcccctgcgc gaagacgccg
     1441 cgctcgaaga gcgtattgga gaggttcatg gccgtgtcgg cgtcgccgac gatgatcggc
     1501 gtgatcggcg tctcgctgcg gccggtgttg aagccgagct gttgcaattg ctccttgagg
     1561 aagcgcgcgt tttgccacag ccggtcgatc aactgcggct cctgctccag cacgtcgatg
     1621 gccgcgatgc aggtcgccgc tacactcggc ggctgcgagg tgctgaagag gaagggccgc
     1681 gccatgtgga tcaggaaatc gcgcagcacc tgctcgccgg ccacatagcc gccgaccgag
     1741 ccgatcgcct tggagagcgt gccgacctgg atctggacgc gcccctccag cccgaagtgg
     1801 ctgacgctgc cgcggccgtt cttgcccagc acacccgagg cgtgggcatc atcgaccatc
     1861 accaccgcgt tgtagcgctc ggccagatcg acgatctggg gcagcggggc gatatcgccg
     1921 tccatgctga agacgccgtc ggtcaccacc aggcgcacgc gggcgctctg cgtctctttg
     1981 agcgcctgct ccaggccgtc catgtccttg tgggggaaga tcttgcgctg ggcgcgcgtc
     2041 aggcggatgc cgtcgatgat gctggcgtgg ttgagctggt cggagatgac cacgtcctcc
     2101 tcgtgcagca ccgactgcaa gacgcccacg ttggcggtga agccggactg gaaggtgagc
     2161 gaggcctcgg tccccttgaa ggcggccagg cgctcctcca gctcctggtg gatccccatc
     2221 gtgccggcga tggtgcgcac ggcgcccgag ccgacgccga actcgcgtgt ggccgcgacg
     2281 gccgcctcga tcagcttggg gtgggtcgtc agcccgagat aattgttcga cgagaggttg
     2341 atcacgcgct ggccgtcgac cgtgaagacc gcctcgttgg ccgtccccac caccctgagc
     2401 ggatgataga gccccttcgc cttcagctcg tcgatctcct gggcgaggaa gcgcatgttc
     2461 ggccgcgtct gtgccacggg cggctccttt ctgggactgc ggcgctcagc gcgcgccgtg
     2521 cgcgtgattt tccgtgggct ccggtggctc cgttggatac aggatgacct tgccggcctg
     2581 gctgtgcaag agcagctcga agccccgctg gaactcggtc agcggcaggc ggtgggtgat
     2641 aatcggcgcg acgtcgacca gcccgcggcc gagcagcgtg ctcgtctggc gccaggtctc
     2701 gaacatcttg cgcccgaaga tgccgtgcac ctcgatcccc ttgagcacca gcaggttgct
     2761 gaagtcgaac tcgaagggtt cgtccgccag cccgagcatc gagatgcggc cgccggggcg
     2821 cagcgccgcg aaggcctgcc gcgtgccgat gggcgagccg ctcatctcga aggccacgtc
     2881 gacgcccgag cccagctcgc gccggatcac cgcgaccggg tcctcttcac gcccgttgat
     2941 cacccgggtc gcgcccatct tgagcgccag ctccaggcgg aagtcgttga cgtcgacggc
     3001 gtagatcggg gcggcgccgc cggcccgcgc gatgccgacc gcgaagaggc cgatcgggcc
     3061 gcagcccatc accagcgccg agcgtgccgt gacctcgccg gagaggacgg tatggaccgc
     3121 gttgccgaag ggctcctgga tggcggcggt gtcgaccggc agatcgggcg ggttgaccca
     3181 ggcgttctcg gccgggatgc agatgtactc ggcgaagcag ccgggccggg tcacgccgac
     3241 cagccccatg cgctcgcaga ggtgcgcctg gccctcgcgg cagcggtcgc agacgccgca
     3301 gccgatgtgc gtctcgccgg agacgaagtc gccgacctgc acatgcgtga cgtcgcgccc
     3361 aacttcgacc acctcgccgc agtactcgtg gccgaagacc atcggggtag ggccgaagtg
     3421 ctcctcggcc caggggtccc agcggtagat gtggagatcg gtgccgcaca tcgaggtggc
     3481 gatgacgcgg atcagggcat cgtggtcgcc ggggcgcggc accggcacct cccgcagctc
     3541 ggcgcccggc gcgcgctcag gcttgacgat cccgagcatc gtctcggtca tggccggtcg
     3601 acctcctccg tcgtggcccc accgtcacgc tcgctcgccc ccatcgcccg gctcgccggc
     3661 gcggccggga cagcgtcgct gcgaacgaac tggtgccctc tttccgcagc ttccgagcca
     3721 ccgcggcgtg tatgccgcag gctgtgcggt tagccacgct ttttgaccgc cgctaagctc
     3781 tcgatccgtg agtagaagtt gcctcaccgc cgaccccttg agccacgcac cgctggcctg
     3841 tccgacgacg gtgggtctgc cgttgtcatc atgctcaccc cgggacaaat ttgtccttcc
     3901 ctcttgacga cctgtcatgc cccctgctag gttgatctcg cgcgaatttc ccgctgctgt
     3961 attggcagaa agctaatacc cgcgatcctg tggggaccca cgctgctggt tgtcgtaggt
     4021 cttctcaggg tctgcatggt agttcatgag gggggccgtt catgcgtggc cgggcggtca
     4081 ttcgccgctt gctgctggcg ctcatcctgc tcgcgcccgg cacacccgcg atcgtcgccc
     4141 aggacgtcgc cgcccacgcc gcgacccaac cgcagactgt gtccgtagcc gcactttcgg
     4201 ccgcgagtgc gccgcccgac gatcgcctgc acctcgccgc cctgcccacg ccctccggcg
     4261 cctct
//
LOCUS       DASUBI010000224         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_945422, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000224 DASUBI010000000
VERSION     DASUBI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945422"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..374)
                     /locus_tag="VFU72_04365"
     CDS             complement(18..374)
                     /locus_tag="VFU72_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422752.1"
                     /translation="MAHEHEHEHEQQQNSPRNPVPPKGKQAGNPGDVGSDPKEKAGGP
                     GTKGVYPVSAHDAPDDAEVQGMASFGQGKRGAEGYFDHGESEIDMQPGKTDQEILNEQ
                     QRSTQQKKSQGKQGQS"
     gene            complement(443..1792)
                     /locus_tag="VFU72_04370"
     CDS             complement(443..1792)
                     /locus_tag="VFU72_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEU5422753.1"
                     /translation="MSEQRPIGAVVEAVYNAAQPLQGTRNDYDKLMDLIGDAQFALIG
                     EASHGTHDFYQQRAEITKRLITEKGFTAVAVEADWPDAYRVNCYVRGRGVDGNADDAL
                     GGFERFPTWMWRNAVVEEFVDWLRDHNEGLANEKQCGFYGLDLYSMYSSIAEVIRYLD
                     QTDPEAAQRARKRYACLEHFGRDAQAYGYATVFGQSETCEQEVVNQLVDLQRHRLELI
                     KQHGEIEEEKFFSAEMNARVVLDSEEYYRTMFYGNVASWNLRDSHMVDTLDSLAEHLG
                     KINGQPPRIVVWAHNSHLGDARATEMGAAGEWNVGELVRERHDGKARLIGFTTYTGTV
                     TAASDWDQPPQRKRVRRGLPGSYEDVFHGTEIPRFYLPLRDGARAAEQLSEPRLERAI
                     GVIYRPETERISHYFLARISDQFDAVIHLDETRALEPLEINAEWHSIEPPETYPFGQ"
     gene            complement(1886..2581)
                     /locus_tag="VFU72_04375"
     CDS             complement(1886..2581)
                     /locus_tag="VFU72_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEU5422754.1"
                     /translation="MPVPFQNRFDAGRQLAQKLLEYVDMPTTDVLGLPRGGVPVAYEV
                     AIQLNQPLDVFLVRKLGVPDHEELAMGAVASGGVRVLNQSVIYGLDISEEAIDDETRR
                     ELAVIQQRERDYRGDQPPLRINGRTIILIDDGLATGSTMLAAVAALRQKNPAKIVVGV
                     PVASREACREVGSHVDQIVCLATPEPFYAVGQWYLEFGETSDAEVRDLLRRARRRAGV
                     WTPPPEEDLPRTG"
BASE COUNT          405 a          939 c          952 g          516 t
ORIGIN      
        1 gagggctccc ccggccgcta cgactgcccc tgcttgccct gcgacttctt ctgctgggta
       61 gacctctgct gctcgttcaa gatctcctga tcggtcttgc ccggctgcat gtcgatctcg
      121 gactcaccgt ggtcgaaata gccctcggcg ccccgcttgc cctgaccgaa cgaggccatc
      181 ccctggacct ccgcatcgtc gggcgcgtcg tgcgccgaca cggggtagac ccctttggtg
      241 cccgggccgc cggccttctc cttgggatcg gagccgacgt caccaggatt gccggcctgc
      301 ttgcccttgg gcggcaccgg gttgcggggc gagttctgct gctgctcgtg ctcgtgctcg
      361 tgctcgtgtg ccaatggtgg ctcccttctg tgcggcggtt cctccgccgc gtcggccgct
      421 gcacgcggcg tgccggtggc tgttactggc caaacggata cgtctcaggt ggctcgatcg
      481 agtgccactc ggcgttgatc tccagcggct ccagcgcgcg ggtctcgtcc agatggatca
      541 ccgcgtcgaa ctggtccgag atgcgcgcga ggaagtagtg gctgatgcgc tcggtctcag
      601 gccggtagat cacaccgatg gcgcgttcca gccgcggctc gctgagctgc tcggccgccc
      661 gggcgccgtc gcgcagcggc aggtagaagc gcgggatctc ggtgccgtgg aagacgtctt
      721 cgtagctgcc gggcagaccg cgacggacgc gcttacgctg cggcggctgg tcccaatcgc
      781 tggcggcggt gacggtgccg gtataggtgg tgaaaccgat caggcgtgcc ttgccgtcgt
      841 gccgctcgcg caccagctcg cccacgttcc actcgccggc cgcgcccatc tcggtagcgc
      901 gggcgtcgcc caggtgcgag ttgtgcgccc agacgacgat cctgggcggc tggccgttga
      961 tcttgcccag gtgctcggcc agcgagtcga gggtatcgac catgtggctg tcgcgcaggt
     1021 tccaggaggc gacgttgccg tagaacatgg tgcggtagta ctcctcggaa tcgagcacca
     1081 cccgcgcgtt catctcggcc gagaagaact tctcctcctc gatctctccg tgctgcttga
     1141 tcagctccaa ccggtggcgc tggagatcga ccaactggtt gacgacctcc tgctcgcagg
     1201 tctcggactg gccgaagacg gtggcatagc cgtaggcctg ggcgtcgcgg ccgaagtgct
     1261 ccaggcaggc gtagcgcttg cgggcgcgct gcgccgcctc ggggtcggtc tggtcgagat
     1321 agcggatcac ctcggcgatc gagctgtaca tgctgtagag gtcgagaccg tagaagccgc
     1381 actgcttctc gttcgccagg ccctcgttgt ggtcgcgcag ccagtcgacg aattcctcga
     1441 ccaccgcgtt gcgccacatc caggtgggga agcgctcgaa gccgcccagc gcgtcgtcgg
     1501 cgttgccgtc cactccgcgg ccacgcacgt agcagttgac ccggtaggca tccggccagt
     1561 cggcttcgac cgccacggcg gtgaagccct tctccgtgat caggcgcttg gtgatctcgg
     1621 cacgctgctg gtagaagtcg tgggtgccgt ggctggcctc gccgatcagg gcgaattgcg
     1681 cgtcgccgat cagatccatt agcttgtcat aatcgttccg ggtgccctga agcggctggg
     1741 ccgcgttgta gacagcctca accaccgcac caataggtcg ttgttcactc atgcgtgcgc
     1801 tcctccctgc tgcatcacaa acgttccgca cacgctaact aagcgcattg gccgtaccaa
     1861 cgagccggtg cggcgagtcg tacgttcagc cggtgcgcgg cagatcctcc tcgggcgggg
     1921 gcgtccaaac gcccgcgcgc cggcgggcgc ggcgcagcag atcgcgcacc tcggcgtcgc
     1981 tggtctcgcc gaactccagg taccactgac cgacagcgta gaacggctcg ggggtggcga
     2041 ggcagacgat ctggtcgacg tggctgccga cctcgcggca ggcctcgcgc gacgccaccg
     2101 gcacgccgac cacgatcttg gccgggttct tctggcgcag cgcggccacg gcggccagca
     2161 tggtcgagcc agtcgccagc ccgtcgtcga tcagaatgat ggtgcggccg ttgatgcgca
     2221 gcggcggctg gtcgccgcga tagtcccgct cgcgctgctg gatgaccgcc agctcgcgcc
     2281 gggtctcgtc gtcgatcgct tcctcggaga tgtctaaccc gtagatcacg ctctggttga
     2341 gcacgcgcac gccgccggag gcgacggcgc ccatggccag ctcctcgtgg tccggaacgc
     2401 ccagcttgcg caccaggaag acgtcgagcg gctgattcag ttggatcgcg acctcgtagg
     2461 ccaccgggac gccgccgcgc ggcaggccga gcacatccgt cgtgggcatg tcgacgtatt
     2521 cgagcagctt ctgggcaagc tggcgcccgg cgtcgaagcg attctggaat ggtactggca
     2581 tggcatgctc caatcgcgtg gcccagctca cagctgggcc tgcccgccgt ctagcgctgc
     2641 aaggcgtgtg ccggtgtggc gagggtggtg gcgaacgagg aacgtggggc gctggtgagg
     2701 acggatcctt cgacaagctc aggatgaccg ggaaatggag atggcctagc ggacaacgct
     2761 catcgtattc gccagcccct acctccccct gtcatcctga gcttgtcgaa gg
//
LOCUS       DASUBI010000225         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_82364, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000225 DASUBI010000000
VERSION     DASUBI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82364"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1246)
                     /locus_tag="VFU72_04380"
     CDS             complement(86..1246)
                     /locus_tag="VFU72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5422755.1"
                     /translation="MPSMTPEMLERIRAEAQARTAAVAALAARICEVPSPTGEESQRG
                     AFVAALLAERGYQTETDDVGNVYTRRGRRGGPLLMLLAHIDTVFPAGTPIAVRQEGDT
                     LHGPGIGDNAVNVASMITLLDVLDALGVETSCDLIACADVGEEGLGNLRGARAACERF
                     REELGAVLVIDGRQGNITNAGVGSKRWLVTVRGPGGHSFGAFGVPSAIHGLGRIIAAI
                     ADIQVPHEPKTTYNVGVIEGGTSVNTIAARASGLLDMRSVGVPELDQLAAKVERIIGT
                     APDPGLTSEIQVVGERPAGSRPLNDPLVQLAISVLRWLGYEPELHASSTDANIPISLG
                     IPAVCVGVARGEGAHTVHEFIEIPSITDGLAQIIRLTIEAGDLVAQGVVHPD"
     gene            complement(1523..>2205)
                     /locus_tag="VFU72_04385"
     CDS             complement(1523..>2205)
                     /locus_tag="VFU72_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5422756.1"
                     /translation="YSYGRYGAQAGIGRLLDSLARHDVRATFFIPGYDAERYPEVMAR
                     IAADGHEPAGHGYAHEDFSALAPEAQEAALARGEAAFERIFGAKPRGWRAPDGLMSLE
                     TRAILARRGYRYDSSYNDDDLPYVVGEGERRLAELPVFWSSSDRNYYALYRQPEVVAA
                     ALIEEFRAMYAVGGLCTLILHPRGDYGSGRQVRLEAVETILQRIREEPSVWLATCGEI
                     ADWALESA"
BASE COUNT          318 a          784 c          766 g          337 t
ORIGIN      
        1 gtgcgacagc gccgtttccc cccaacgatg cgtaggacaa cgcgggcaga cacggaggtc
       61 tgcccctaca acgagatgac ggccctcaat ccggatgcac cacgccctga gcgacgagat
      121 cgccggcctc gatcgtcagg cggatgatct gggccagacc gtcggtgatc gaggggatct
      181 cgatgaactc gtgcaccgtg tgcgcgccct cgccgcgcgc cacgccgacg cagacggccg
      241 ggatgcccag gctgatcggg atgttggcgt cggtgctgga ggcgtgcagc tccggctcgt
      301 agcccagcca gcgcagcacg ctgatcgcca actgcaccag gggatcgttg agcgggcgac
      361 tgccggccgg gcgctcgcct actacctgga tctcgctggt cagccccgga tcgggcgcgg
      421 tgccgatgat tcgctcgacc ttggcggcca gctgatccag ctccggcacg ccgaccgagc
      481 gcatgtcgag taggccagag gcgcgagccg cgatggtgtt gaccgaggtg ccgccctcga
      541 tcacgccgac gttgtaggtc gtcttcggct cgtgcggcac ctggatgtcg gcgatggcgg
      601 cgatgatcct gcccaggccg tggatcgcgc tgggcacgcc gaaggcgccg aaggagtgcc
      661 cgccggggcc gcgcaccgtc accagccagc gcttcgagcc gacgccggcg ttggtgatgt
      721 tcccctgccg tccgtcgatc accagcaccg cacccagctc ttcgcgaaag cgctcgcagg
      781 ccgcccgcgc gccgcgcagg ttgccgagcc cctcctcgcc gacatcggcg caggcaatca
      841 ggtcgcagga ggtctcgacg ccgagcgcgt cgagcacgtc gagcagggtg atcatcgagg
      901 cgacgttgac cgcgttatcg ccgatgccgg ggccgtgcag cgtgtcgccc tcctggcgca
      961 cggcgatcgg cgtgccggcg gggaagacgg tgtcgatgtg cgccagcagc atcagaaggg
     1021 gcccgccacg ccggccacgt cgggtgtaga cgttgccgac gtcgtcggtc tcggtctggt
     1081 agccgcgctc ggccagcagc gcggcgacga aggcgccgcg ctgcgactcc tcgccggtcg
     1141 gcgatggcac ctcgcagatg cgcgccgcca gggccgccac cgccgccgtg cgcgcctgcg
     1201 cctcggcgcg aatccgctcc agcatctcgg gtgtcatgct cggcatggtc ctccttggtg
     1261 tttgctgccc tcacccccga cccctctccg gacggagccg tgcgcgggcg aggggcgctg
     1321 cccatcgaag agattggacg ctttcgagga cggatccttc gacttcgctc aggatgacag
     1381 ggtaggagag agacgtacga cagcgttgtt tgtcgttgtc agctcctctt gaacctggtc
     1441 atcccgagcg cagtcgaggg atccatcttg ccctatcacc ccagcaacac ctttcgccac
     1501 ctctggcggg tgaaggcccc cttcaggccg actccagcgc ccagtcggcg atctcgccgc
     1561 aggtggccag ccagacgctc ggctcctcgc ggatgcgctg caggatcgtc tcgactgctt
     1621 ccaggcgcac ttggcggccg gagccgtagt cgccgcgcgg gtgcaggatc agggtgcaga
     1681 gcccgcccac ggcgtacatg gcgcggaact cctcgatcag cgcggcggcc accacctccg
     1741 gctggcggta gagcgcgtag taattgcggt cgctcgacga ccagaagacc ggcagctcgg
     1801 ccagccgccg ctcgccctcg ccgaccacgt acggcaagtc gtcgtcgttg tagctgctgt
     1861 cgtagcggta gccgcgccgg gccaggatgg cgcgcgtctc caggctcatc aggccatccg
     1921 gcgcccgcca gccgcgcggc ttcgcgccga agatgcgttc gaaggccgcc tcgccgcgcg
     1981 ccagcgccgc ctcctgcgcc tcgggcgcca gcgcgctgaa gtcctcgtgg gcatacccgt
     2041 gcccagctgg ctcgtgcccg tcggccgcga tacgcgccat gacctccggg tagcgctcgg
     2101 cgtcgtagcc ggggatgaag aaggtggcgc gcacgtcgtg gcgcgccagg ctgtcgagca
     2161 ggcggccaat cccggcctgg gcgccgtagc ggccgtagga gtagc
//
LOCUS       DASUBI010000226         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_696716, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000226 DASUBI010000000
VERSION     DASUBI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696716"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFU72_04390"
     CDS             complement(<1..372)
                     /locus_tag="VFU72_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5422757.1"
                     /translation="MDIELGKPVVTEGGKQIGSVDGLIFDPHSKELVQLLVKQGTLLS
                     KDRIAAIDEVASVDGDGTVHLSIPDSMADQLPAFVEREFVIVKPHEYPDLPPSFAGGT
                     GAGLLFWGGSRLGLGYDPHSQY"
     gene            complement(463..687)
                     /locus_tag="VFU72_04395"
     CDS             complement(463..687)
                     /locus_tag="VFU72_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422758.1"
                     /translation="MASPLTPSDPVRSGLPVDAFLMYVGGVSIFALLWLYYGLRHFQA
                     QRSGIALVGYGSVALSIIVVVIVFIVGAIR"
     gene            complement(835..1623)
                     /locus_tag="VFU72_04400"
     CDS             complement(835..1623)
                     /locus_tag="VFU72_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5422759.1"
                     /translation="MELTRILWEIEGGVGVATLNRPERLNALDLRTIVELDTVAERAW
                     NNPEIRCLVITGTGRGFSAGADVKEWSSSAEEREPDEDGWVPRMHRLMARLYRMPKPV
                     IAAVNGVAVGGGCDLALVADLRIASTAARFGEAYIRVGFCPDAGGSFLLPRLIGPAKA
                     AELIFTGRIIDAPEAQQLGLLNALVEPEELMPTAMEWARRLASLPTVAIGLAKENIHA
                     NLDLTFEEALRNERRAGQICGATEDHREGLRATVEKRQPVFTGR"
     gene            2102..2398
                     /locus_tag="VFU72_04405"
     CDS             2102..2398
                     /locus_tag="VFU72_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422760.1"
                     /translation="MDEHAHEDQLSHLSADELRERLEKGELTPQQVDQALQQQARRAA
                     AQRSSDELLPDEHVTAEGFGSGQGMGTARTGQGPNRPDEEGFPRTQKDKEDWPS"
     gene            complement(2416..3948)
                     /locus_tag="VFU72_04410"
     CDS             complement(2416..3948)
                     /locus_tag="VFU72_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5422761.1"
                     /translation="MRQTNNRLVVVAVLLATFLSALDTSIIGTAMPTVIGQLGGLALY
                     SWVFSAYLLTSTTAVPIYGRLADLYGRKPLAFSAIALFLLGSALCGLAQNMPELVAFR
                     ALQGLGAGGIIPITMTILGDIFAAEDLAKVQGLTSAVWGSSAIAGPTVGGFIVDYVGW
                     RWVFYVNLPFGIAAAALLWLFFHEQVERRPHRIDYLGALTMTLSITSLLLALLRVGQD
                     RAWLDPTVIGPFVLAVLLLGWFLWNEAHAAEPMLPLGLFRLRIISVVFPANVLIGAAM
                     FGVTSYLPLFVQGVLGGSAIDAGLVLAPYSVCWSLASVLSGRILIRFGYFTSAFAGFL
                     VMLAGAVLLQLVPGSQTLWPSIVSAAIFGCGMGLSATAFIIAAQNAVGWSERGAATAS
                     VMFGRTIGGAIGVAALGAVLSAAMAPRLASLGGHYDANALLDPAARAALAPDILQAIQ
                     GALQHGLQLAYLGVLGGVVVAVLVVLAFPRGSVAQLQVADESRGGGTATPVREPATLR
                     ED"
     gene            complement(3932..>4670)
                     /locus_tag="VFU72_04415"
     CDS             complement(3932..>4670)
                     /locus_tag="VFU72_04415"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5422762.1"
                     /translation="ALSDAFVFRALAGLGFAVQGVAVVALAGQLARGQPGSGADDNAS
                     GVAALLGAARALEDERPEEVETWLLFTSGADDGRTGMVQFLGENQFDPTTTDFIDVDR
                     VGSAQLCYTRGEGLVAPLRSNARLLALAEEVAAAHVDWGVGATVSRWPGNQFATLIRG
                     YPALAITGCGIRRWRAGADTVNTLDIESVAHAAALVLGIIRRLDREVRLGAVEEVEIW
                     QPGQSPAAPDEPESTDEEAMGERAADQ"
BASE COUNT          788 a         1597 c         1638 g          647 t
ORIGIN      
        1 gtactgggag tgcggatcgt agcccaggcc gagccggctg ccgccccaga acaacaggcc
       61 ggcgccggtg ccgccggcaa acgagggtgg caggtcggga tactcgtgcg gcttgacgat
      121 gacgaactcg cgctccacga aagccgggag ctgatcggcc atgctgtcgg ggatactaag
      181 atgaaccgtg ccgtcgccgt cgacgctggc gacctcgtcg atcgccgcga tgcggtcctt
      241 ggacaacagc gtgccctgct tgaccagcag ttgcaccagc tctttcgagt ggggatcgaa
      301 gatcaagcca tcgaccgagc cgatctgctt gccgccctcg gtcacgaccg gttttccaag
      361 ctcgatgtcc acggcagtct cctctcccgc gcagcgatca acaccacagg cgtgtttgac
      421 tgccgtcgcg cgtctgcaac ttcgatgcca cccgagggcg ggctagcgaa tcgcgccgac
      481 gatgaagacg atgacgacga cgatgatcga gagcgccacg ctgccgtagc cgaccagcgc
      541 gattccgctg cgctgcgcct ggaagtgcct cagcccgtag tagagccaga ggagggcgaa
      601 gatcgagacg ccgcccacgt acatcaggaa ggcgtcgacc ggaaggcccg accggaccgg
      661 gtcactgggg gttagcgggc ttgccacgcg cgaacgctcc tctcaccgac gagcggttcc
      721 agccgctcgc cgcgtcaatc gttctcggcc ggctacggcg catacggacg cgtgcgcccg
      781 ccgcgcactc gtagcctacg ccaactccgg cccggcgtca ccccctttgg aggtctagcg
      841 gccggtgaag accggctggc gcttctcgac cgtggcgcgc aggccctcgc gatgatcctc
      901 ggtggcgccg cagatctgcc cggcgcgccg ctcgttgcgc agcgcctcct cgaaggtgag
      961 gtcgagattg gcgtggatgt tctccttggc cagcccgatc gccaccgtcg gcagcgaggc
     1021 gagccgccgc gcccactcca tcgcggtggg catcaactcc tcgggctcga ccagggcgtt
     1081 gagcagcccc agttgttggg cctccggcgc gtcgatgatg cgcccggtga agatcagttc
     1141 ggcggccttg gccgggccga tcaggcgtgg caggaggaag gagccgccgg catcggggca
     1201 gaagccgacg cggatatagg cctcgccgaa gcgcgcggcg gtcgaggcga tacgcaggtc
     1261 ggccaccagc gccaggtcgc agccgccgcc gactgccacc ccgttgaccg cggcgatgac
     1321 gggcttgggc atgcggtaga ggcgcgccat cagccggtgc atgcgcggta cccaaccgtc
     1381 ctcgtccggc tcgcgctcct cggcagagct gctccactcc ttgacgtcgg cgccggccga
     1441 gaagccgcgt ccggtgccgg tgatcaccag acagcggatc tcggggttgt tccaggcgcg
     1501 ctcggccacg gtgtccagct cgacgatggt gcgcaggtcg agcgcgttga gccgctcggg
     1561 ccggttcagc gtggccacgc cgacgccgcc ctcgatctcc cagaggatgc gtgtcagctc
     1621 catcaagtgc tcctctccat ctgctcttgc acaggtccgg ctccagtgta gtacggaata
     1681 ccggagagtg gaccggcgcg cggggcgggc gcctgaggcg gccgtcggcg agcaacgacg
     1741 ctccggtgaa aacgccgggc aattcgccag ctctccgcgc gatcgtcacg atgcgccacg
     1801 gctgcccagg tatgagttcg ttcgcttggt taaaggcgac gcggcgtgct gggggacacc
     1861 gcacgccgcg tcaagggaag ggaagggatt gggttttccg ggttgtgaca cttgcgtgcc
     1921 gccccggtgc ctacatccta tcatttgaca taaaccttgt caaatggtgg ggccgggaac
     1981 aacccgtcgc atccctctat ggcaggttcc gtgccacggt gactccctgt catctctcgg
     2041 cgtcccgaat cgggaccagg gtacggctct tgcatcctcg gacctgacgt aaggagggat
     2101 catggacgag cacgcacacg aggatcagtt gagtcacctg agcgcggacg agctgcgcga
     2161 gcggctggag aagggcgagc tgacgcccca gcaggtcgac caggctctgc aacaacaagc
     2221 acggcgtgcg gcggcgcagc gctcatccga cgagctgctg ccggacgagc acgtcaccgc
     2281 cgaaggcttc ggctcggggc aggggatggg caccgcgcgc actgggcagg gtccgaatcg
     2341 ccccgatgaa gagggtttcc cgcgcacgca gaaggacaag gaagactggc cgtcgtagtg
     2401 acgcgcctgc tccgctcagt cttcccgtag ggtggccggc tcgcggaccg gggtggctgt
     2461 cccgccgccc cggctctcgt ctgccacttg cagttgcgcc acgctcccgc gcgggaatgc
     2521 cagcaccacc agcaccgcta ctaccacccc gcccagcacg ccgagatagg cgagctggag
     2581 gccgtgctgc agcgcgccct ggatcgcctg caggatatcc ggcgccagcg cggcgcgggc
     2641 ggcggggtcg agcagcgcgt tggcgtcgta gtggccgccg aggctggcca gccggggagc
     2701 catggccgcg ctgagcacgg cgcccagcgc cgccacgccg atcgcgccgc cgatggtgcg
     2761 cccgaacatc accgacgccg tggcggcgcc gcgctcggac cagccgaccg cgttctgggc
     2821 cgcgatgatg aaggccgtag ccgagagtcc catgccgcag ccgaagatcg cggccgagac
     2881 gatgctcggc cagagcgtct ggctgccggg caccaactgt agcaggaccg cgccggccag
     2941 catcaccagg aagcccgcga aggccgaggt gaagtagccg aagcgaatca ggattcgccc
     3001 cgagaggacc gaggccagcg accagcagac cgagtagggg gccagcacca gcccggcgtc
     3061 gatggcgctg ccgccgagca cgccctgcac gaagagcggc aggtaggatg tgacgccgaa
     3121 catagccgcg ccgatcagca cgttggccgg aaagaccacg ctgatgatgc gcaggcggaa
     3181 gagcccaagc ggcagcatcg gctcggccgc gtgcgcctcg ttccagagga accagcccag
     3241 caggagcacc gccagcacga acgggccgat gaccgtgggg tcgagccagg cgcggtcctg
     3301 cccgacgcgc agcagcgcga gcaacaggct cgtgatactc agcgtcatgg tcagcgcgcc
     3361 gaggtagtcg atgcggtgcg gccggcgttc gacctgctcg tggaagaaga gccagaggag
     3421 ggcggccgcc gcgatgccga aggggaggtt gacgtagaag acccagcgcc aaccgacgta
     3481 gtcgacgatg aagccgccga ccgtcggccc ggcgatggcc gacgagcccc agacggcgct
     3541 ggtcagcccc tggaccttgg ccagatcttc ggcggcgaag atgtcgccca ggatggtcat
     3601 ggtgatgggg atgatgccgc ccgcgccgag cccttgcagg gcgcggaagg cgaccagctc
     3661 cggcatgttc tgcgccaggc cgcagagcgc cgagccgagc aggaagaggg cgatggcgct
     3721 gaaggcgagc ggcttgcgcc cgtagaggtc ggccaggcgc ccgtagatcg gcacggcggt
     3781 ggtcgaggtc agcaggtagg ccgagaaaac ccacgagtag agcgcgagac cgccgagctg
     3841 cccgatgaca gtcggcatcg ccgtgccgat gatgctggtg tcgagcgccg agaggaacgt
     3901 cgccagcagc accgccacca cgacgagccg gttattggtc tgccgcacgc tcccccatcg
     3961 cttcctcgtc cgttgactcg ggttcatcgg gcgccgccgg gctctggccc ggttgccaaa
     4021 tctccacctc ctcgaccgcg cccaagcgca cctcgcgatc gagccttcgg atgatgccga
     4081 ggaccagagc ggcggcgtgc gcgacgcttt cgatgtcgag agtattcacg gtatccgccc
     4141 cggcgcgcca gcgccggatg ccgcagccgg tgatcgcgag cgccggatag ccgcggatca
     4201 gcgtggcgaa ctgattgccg ggccagcgcg agacggtcgc gccgactccc cagtcaacgt
     4261 gcgcggcggc gacctcctcg gccagcgcga gcagccgcgc gttcgagcgg agcggcgcga
     4321 ccagcccctc gccacgggtg tagcagagct gcgcgctacc gacgcggtcg acatcgatga
     4381 agtcagtggt ggtcgggtcg aattggttct cgccgaggaa ctgcaccata ccggtgcggc
     4441 catcgtcggc gccggaggtg aagaggagcc aggtctcgac ctcctccggc cgctcgtcct
     4501 ccaacgcgcg cgccgcgccc agcagcgcgg ccacgccgga ggcattgtcg tcggcgccgg
     4561 agcctggctg gccgcgcgcc agttgcccgg ccagcgccac caccgcgact ccctgcaccg
     4621 cgaagccgag gcccgccagg gcgcggaaga cgaacgcgtc gctcagcgcc
//
LOCUS       DASUBI010000227         5555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_492201, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000227 DASUBI010000000
VERSION     DASUBI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492201"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFU72_04420"
     CDS             complement(<1..985)
                     /locus_tag="VFU72_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEU5422763.1"
                     /translation="MSDNKWLRNGFVWIVLIIAVVALWFTFMGSGGNPGTVDFAAVAT
                     DIQKGKVERLDTHDNSSEITVHYYGGETKSTRLPPGNVNVWQALSYFDIPADKVPVKA
                     NPGSSWGSWIGALSILIPVLFLGGMIILMMRQAQGSNNQAMSFGKSRARMFTGTKPTV
                     TFGDVAGVDEAKEELAEVVEFLKYPDKFASLGARIPRGVLLVGPPGTGKTLLSRAVAG
                     EAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFVDEIDAVGRQRGAGLG
                     GSHDEREQTLNQILVEMDGFDTNTNVIVVAATNRPDVLDPALLRPGRFDRQV"
     gene            complement(1118..1684)
                     /gene="hpt"
                     /locus_tag="VFU72_04425"
     CDS             complement(1118..1684)
                     /gene="hpt"
                     /locus_tag="VFU72_04425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5422764.1"
                     /translation="MGSKIRDIPLHRGLTKVLIDEATLARRVAELGREIGEFYRGREP
                     ILIGVLTGAVVFMADLARAMEIPLTMEFLAISSYGNATRSSGVVRILKDLNHPIEGKP
                     VLVVEDIIDSGLTLDYLLDLLQRRNPSEIRVATLLLKQRARQIEVKPDWVGFEIPDEF
                     VVGYGLDYAGRYRNLPFIGIGSADAIAS"
     gene            complement(1665..2714)
                     /gene="tilS"
                     /locus_tag="VFU72_04430"
     CDS             complement(1665..2714)
                     /gene="tilS"
                     /locus_tag="VFU72_04430"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5422765.1"
                     /translation="MSDPLYQRVRRCLLDAGLGPGDRVVVGFSGGVDSLVLLHLLQAL
                     HERQAGPEPLALHVDHGLRPQSAADAERAVEMAREIGAPCRVAVVDVARWDVATIGVE
                     AAARHARYAALAGEARRASAAVLAVAHNRDDQAETVLLRLLRGAGLHGLAAMRAVSRR
                     EVLLAPRGESMSVTLVRPLLETARAEIEGYAATHGLRPIEDPSNREGVFRRNRVRHEL
                     LPLAEALVPGARATIARSAALLADDAALLAQLADEAAAAVLREDGASLMLSRDPLLAL
                     PVALQRRVLLRAWARQDAASEPSFERVEALRVALRRGVPATIELGGGLTARVDHAGAR
                     LRHIGKESVGGEQDP"
     gene            complement(2931..3356)
                     /locus_tag="VFU72_04435"
     CDS             complement(2931..3356)
                     /locus_tag="VFU72_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5422766.1"
                     /translation="MGLRRVWERIGRQERPAPVRRGPAPRVAIYAREERDAEGRLVLG
                     VEAQEEECRVFAARQGWEIVATFADRSTEPEAGRDGLKALRGAIWRRRFDAVVSSTPD
                     RLYYDIERLVLLAREARLLGVELIFVEAPRDLDLYGEDF"
     gene            complement(3358..3840)
                     /locus_tag="VFU72_04440"
     CDS             complement(3358..3840)
                     /locus_tag="VFU72_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422767.1"
                     /translation="MAEQVEAVEAVGTGGPFVQLAVLCEHAQQDQYGSLSVLNVLEQL
                     VAGSSDPKAPDVMPEFRFQADLVVAFASGTVSGERTLSIVPEQPSGERLEPVAQVVEF
                     RGDDSRVTFVSNLSIDIGQPGVYWFDVLLDEALVTRVPLRVRYERNVPPRWKVYNLEY
                     "
     gene            complement(4106..5431)
                     /locus_tag="VFU72_04445"
     CDS             complement(4106..5431)
                     /locus_tag="VFU72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5422768.1"
                     /translation="MATTVSAPRYARSRALFAESQQYLAGGNSRLTVFFQPHPLFMER
                     GSGARIYDVDGNEYLDIINNYTSLIHGHAQPEVVEAAIGAVRSGTAFAAPTPPEVGLG
                     KLICERIPAVERVRFVNSGTEAVMMAVKAARAYTGKPAIAKFEGAYHGAYDFVEVSLA
                     SAPANWGAATQPASTRYAAGQPQSVLDEVVALPFNQLEETRALLRQHQGRLAAVLVDV
                     MPSRVGLIPGSDEFLQGLQAECRALGILLILDEVISLRLGYHGAQGELGLEPDLTTMA
                     KIIGGGFPVGAVGGKETVMAVFDPTSGKPALPHGGTFNANPVTMAAGLRAMELMTPEE
                     FERINALGAQARQGLADVVRLLEVGWQVTGAGSLFKLVPSTGPLSDYRSAFVTPEQAA
                     RIHALHWALLDRGVFMGHDALGCISTPMTEADVDQIIHAVEDALRQLEE"
BASE COUNT          879 a         1983 c         1875 g          818 t
ORIGIN      
        1 tcacctggcg gtcgaagcgg cccggtcgca gcagggcagg gtccagcacg tcggggcggt
       61 tggtcgcggc gacgacgatg acgttggtgt tggtgtcgaa gccgtccatc tcgaccaaga
      121 tctggttgag ggtctgctcg cgctcgtcgt gagagccacc aagcccggcg ccgcgctgcc
      181 gcccgacggc gtcgatctcg tcgacgaaga cgatgcaggg ggcgttgcgc ttggcctggt
      241 cgaagaggtc gcgcacgcgg ctggcgccga cgcccacgaa catctcgacg aactcggagc
      301 cgctgatgct gaagaagggc acgccagcct caccggccac ggcgcgggag agcagcgtct
      361 tgccggtgcc cggcgggccg accagcagca cgccgcgcgg gatgcgcgcc ccgagcgagg
      421 caaacttgtc cggatacttg aggaactcga cgacctcggc taattcttct ttggcttcgt
      481 cgacccccgc gacatcgccg aaggtgacgg tcggtttggt cccggtgaac atgcgcgccc
      541 ggctcttgcc gaacgacatc gcctggttat tcgagccctg cgcctgacgc atcatcaaga
      601 tgatcatgcc gcccaggaag aggaccggga tcaggatgct cagcgcgccg atccaactgc
      661 cccaggagct gccgggattg gccttgaccg gaactttgtc ggccgggata tcgaagtagc
      721 tcaacgcctg ccagacgttg acgttgcccg gcggcagccg cgtcgacttg gtctccccgc
      781 cgtagtagtg gacggtgatc tcgctggagt tgtcgtgggt gtcgagccgc tcgaccttcc
      841 ccttctggat gtcagtggcg acagcggcaa aatcgaccgt tccgggattg ccgccgctcc
      901 ccataaaggt gaaccagagc gcaacgacgg cgatgatcag gacgatccag acaaagccgt
      961 tgcgcagcca tttgttgtcc gacatgcgag ccctccgacc gcgtcgatcg cgctctcggt
     1021 accctgacgg tgggccacag gcgtgcggac cagtcatggg tgccggaatt ataacagaca
     1081 gccgatacgc gctcgccgcg ggacgtgtcg cgcgggctca ggaggcgatc gcgtcggcac
     1141 tgccgatgcc gatgaagggg agattgcgat agcggccggc gtagtcgagc ccgtagccga
     1201 ccacgaactc gtccgggatt tcgaagccga cccaatccgg cttgacctcg atctggcgcg
     1261 cgcgctgctt gagcagtagg gtcgcgactc gaatctcgga cgggttgcgg cgctgcagca
     1321 ggtcgagcag atagtcgagc gtcaagccgc tgtcgatgat gtcctcgacc accagcactg
     1381 gtttgccctc gatcggatgg ttcaagtcct tgaggatacg caccacgccg ctgctgcggg
     1441 tggcgttgcc gtagctagag atcgccagga actccatcgt cagcgggatc tccatggcgc
     1501 gggccagatc ggccatgaag acgactgctc cggtcagcac gccgatcagg atcggttccc
     1561 ggccgcggta gaactcgccg atctcgcgcc ccagctcggc cacacgtcgc gccagcgtcg
     1621 cctcgtcgat cagaaccttg gtcagccccc gatgcagggg gatgtcacgg atcttgctcc
     1681 ccacccacgc tctccttccc gatgtgccgc agccgcgcgc ccgcgtgatc gacacgcgcc
     1741 gtcagcccgc cgcccagctc gatcgtcgcc gggacgccac gccgcagcgc gacccgcagc
     1801 gcctcgacgc gctcgaacga cggctccgac gcggcgtctt gccgcgccca ggcccgcagc
     1861 agcacccgcc gttgcagcgc cacggggagc gccaggagcg gatcccgcga gagcatgagc
     1921 gacgcaccat cctctcgcaa caccgccgcc gccgcctcgt cggcgagctg tgccagcagg
     1981 gccgcgtcgt cggccagcag agcggcggag cgggcgatag tcgcgcgcgc ccccggcacg
     2041 agcgcctcgg ccagcggcag cagctcgtgg cgcacccggt tgcgccggaa cacgccctcc
     2101 cggttcgagg gatcctcgat cgggcgcagg ccgtgcgtcg cggcgtagcc ctcgatctcg
     2161 gcgcgtgctg tctccaacag cggacgcacc agcgttacgc tcatcgactc cccgcgcggc
     2221 gccagcagca cctcccggcg cgaaacggcc cgcatcgccg ccagcccatg gagccccgcg
     2281 ccgcgcagca gccgcagcag caccgtctcg gcctggtcgt cccgattgtg cgcgaccgct
     2341 agcacggcgg cgctcgcccg gcgcgcttcc cccgccagcg cggcgtagcg cgcgtgccgt
     2401 gccgccgcct ccaccccgat agtggccacg tcccagcgtg ccacatcgac cacagccacg
     2461 cgacacggcg cgccgatctc gcgcgccatt tcgaccgcac gctcggcgtc ggccgccgac
     2521 tgcggccgca ggccatgatc gacgtgcagc gccagcggtt ccggcccggc ctggcgctcg
     2581 tgcaaggcct gcagcagatg caggagcacc agcgaatcga ccccgccgga gaagccaacg
     2641 acgacccggt cgcccgggcc gaggccggca tcgagcaggc aacgccgcac gcgctgatac
     2701 aaaggatcgc tcatcggcgc tggccagcct gatataggcg gcgatcgaca agatggcgat
     2761 ggagggcaag acggatcctt cgaccgcgct caggatgaca ggctagggtt tgtgaccttc
     2821 cgaacgacga tgatcgtcgc ccgccccaaa ttgccatgca tgtcatcctg agcgcggtcg
     2881 aaggatccgt cttcacgcgg cacaaatcct acttgactgg cagcggcccg tcagaaatct
     2941 tcgccgtaca ggtcgaggtc gcgcggcgcc tcgacgaaga tcaactcgac gccgagcaag
     3001 cgggcctcgc gcgccagcag caccaggcgc tcgatgtcgt agtagagacg atcgggagtg
     3061 ctgctgacaa cggcgtcaaa gcgccgccgc cagatagcgc cgcgcagcgc cttcagcccg
     3121 tcgcggccgg cttccggctc ggtgctccgg tcggcgaagg tggccacgat ctcccaaccc
     3181 tgacgcgcgg cgaagacgcg gcactcctcc tcttgcgcct cgacgccgag caccagccgc
     3241 ccctccgcat cacgctcctc gcgcgcgtag atcgcgacgc gcggggcagg cccgcgcctc
     3301 accggcgccg gccgctcctg ccgcccgatt cgctcccaga cccgtcgcag gcccacctca
     3361 atactccagg ttatagacct tccagcgcgg cggcacgttg cgctcgtagc gcacgcgcaa
     3421 gggcacgcgc gtgaccaggg cctcgtcgag cagcacgtcg aaccagtaga cgccgggctg
     3481 gccgatgtcg atcgagaggt tcgagacgaa ggtcacgcgg ctgtcgtcgc cgcggaactc
     3541 gaccacctgc gcgaccggct ccagtcgctc accggagggc tgctcgggca cgatgctgag
     3601 cgtgcgctcg ccgctgaccg tgccggaggc gaaggccacc accaggtcgg cctggaagcg
     3661 gaactccggc atgacatcag gcgccttggg gtcgctggat ccggccacca gttgctcgag
     3721 cacattgagc acgctgagcg agccgtactg gtcctgctgc gcgtgctcgc agaggacggc
     3781 cagctgcacg aacggcccgc ccgttcccac ggcctcgact gcctcgactt gctccgccaa
     3841 gctgcctccc gcctccaggg cggctgcgcg ccgcgccact accacgaatg actttaccct
     3901 atgggaggcg gcggtgcagg ggaagtggag tacgtgctgt tgaggtaggg gcagacccct
     3961 gtgtctgccc gcctggtgag gcactgatcc tcacggtcgt cacccacctt ccctatttct
     4021 ccgttgccga cgcgcgggac agcgagggcg gccacagggg gccgccccta caagcgttgg
     4081 tcgacgggcc aggggtgagt gcccctcact cctcgagctg ccgcagcgcg tcctccacgg
     4141 cgtggatgat ctggtcgacg tcggcctcgg tcatcggggt cgagatgcag ccgagggcat
     4201 cgtggcccat gaagacgcca cggtcgagca gcgcccagtg cagggcgtgg atgcgcgccg
     4261 cctgctccgg cgtgacgaag gccgagcggt agtcgctgag cggcccggtc gagggcacca
     4321 gcttgaagag cgagccggcg ccggtcacct gccagccgac ctccagcagc cgcaccacgt
     4381 cggccagccc ctgccgcgcc tgcgcgccga gcgcgttgat gcgctcgaac tcctccggcg
     4441 tcatcagctc catggcgcgc agcccggcgg ccatcgtcac cgggttcgcg ttgaaggtgc
     4501 cgccgtgggg cagcgcgggc ttgccgctgg tggggtcgaa gaccgccatg accgtctcct
     4561 tgccgccgac cgcgcccacc gggaagccgc cgccgatgat cttggccatg gtggtcaggt
     4621 ccggctccag ccccagctcg ccctgcgcgc cgtgatagcc gagccgcagc gagatgacct
     4681 cgtcgagaat gagcaggatg ccgagcgcgc ggcactccgc ttgcagcccc tgcaggaact
     4741 cgtcgctgcc ggggatcagg ccgacgcgcg agggcatgac gtcgaccagc acggctgcca
     4801 gccggccctg gtgctggcgc aaaagcgcgc gcgtctcctc gagctggttg aagggcagcg
     4861 cgacgacctc gtcgagcacg ctctgcggct gaccggcggc gtagcgcgtc gaggccggct
     4921 gggtggccgc gccccagttg gccggcgccg aggccagact cacctcgacg aagtcgtagg
     4981 cgccgtggta ggcgccctcg aacttggcga tggccggctt gccggtgtag gcgcgcgcgg
     5041 ccttgaccgc catcatcacc gcctcggtgc ccgagttgac gaagcgcacc cgctcgacgg
     5101 cggggatgcg ctcgcagatc agcttgccca gcccgacctc gggcggggtc ggcgcggcga
     5161 aggcggtgcc cgagcgcacg gcgccgatgg cggcctcgac aacctccggc tgcgcgtggc
     5221 cgtggatcag cgaggtgtag ttgttgatga tgtcgagata ctcgttgccg tcgacgtcgt
     5281 agatgcgcgc cccgctgccg cgctccatga agagcgggtg gggttggaag aagaccgtca
     5341 ggcgggagtt gccgcccgcg agatactgct gggactccgc gaagagcgcg cgcgagcgcg
     5401 cgtagcgggg tgccgaaacc gtggttgcca tgtcgcgtcc tcccccgacg atggtgacgc
     5461 cctgccgatg ccgccacgct agcacacccg gcccctcctg actacggccg ttgcggccag
     5521 gaccaccggc tttcagcgcc atcttgccgc gcgtc
//
LOCUS       DASUBI010000228         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_830923, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000228 DASUBI010000000
VERSION     DASUBI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830923"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFU72_04450"
     CDS             <1..1031
                     /locus_tag="VFU72_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5422769.1"
                     /translation="LPLGDGPIHYPLSVGGTPLVASPALRERLGLPQLWLKDETRGPT
                     GSNKDRASALVLELALRERRDTVSCASTGNVAVSLAVGAAAAGRRAVIFVPAQVSPSN
                     LTLMLLAGATVVKVRAGYKAAFRLSEEAAERFGWFSRNTGVNPVTLEAKKTVAFEVWE
                     QLNRDLPDVVVAPVGDGVTLAALAKGFRELIACGVTARLPRIIGVQAEGSAPIARAWA
                     TDRPLEAIEPNTLADGIAVGAPVSAGEVLRDVRASGGGMVTVSDETLLDWIGILAREA
                     GLLAEPAGVAGTAGVAAALEQGLLDWSERVVALVSGTLLKNLQFARPTRKPIEVEGEL
                     GELERALG"
     gene            complement(1040..1273)
                     /locus_tag="VFU72_04455"
     CDS             complement(1040..1273)
                     /locus_tag="VFU72_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422770.1"
                     /translation="MPYSLPTREDDPVHLVRTIARLAQMLVELRDEYVRSPREDTLDQ
                     IERRLDELSVLRQQLRERRDEQRLHDLEDDGGK"
     gene            complement(1293..3341)
                     /gene="ligA"
                     /locus_tag="VFU72_04460"
     CDS             complement(1293..3341)
                     /gene="ligA"
                     /locus_tag="VFU72_04460"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5422771.1"
                     /translation="MDEANLRRRVDELRALIDRYNYEYYILDQSSISDAEYDALLNEL
                     RRIEAEHPELVTPESPTQRVGATPSQGFGTVRHELPMLSLGNVFSEQDLREWAERVFK
                     LAGRRDLQFVTEPKIDGSAVSILYRDGAYVRGATRGNGIEGEDVTPNIRTIRNLPLRL
                     RPIDDTPVPELLEARGEVYMRKRDFEELNRVRGDAGESLYANPRNSASGALRQIDPAV
                     TANRPLRFFAWDVGIVEGEARPTHSGNLDMLAALGLPVVPDYRVWDSTDEVWAEIARW
                     QSVRHDLPFQIDGVVVKVNEVELQRELGIVGREPRWATAYKFPAIQKTTVLKEIQVNV
                     GRTGTLNPRAILEPVEIGGVVVRHATLHNEDEIRRLGLMLGDTVVVERAGDVIPKIMS
                     VVEPARTGAEIPWEMPTHCPVCGAETVRLPGEAMRYCPNASCPAQLEEEVKHFVSRGA
                     LDIEGIGGKTAERFVRLGLITWLPDIYHLDWGAISELEGFGEKSIENLQASIEASKQK
                     PLSRLLAGLNVRHVGERNAELLARHFGSMERLMAADTEELQTIPGIGGVVADAVYDFF
                     HLPRNVEMITALRDAGLRMSEPVESQPPASAVLAGKTVVVTGRLEGLTRSQAEELLRQ
                     AGANVTSSVTRKTDLVVVGEEPGSKATRARELGVPIVGEAELLRMLNGLASGEDEAAR
                     "
     gene            3475..>5030
                     /locus_tag="VFU72_04465"
     CDS             3475..>5030
                     /locus_tag="VFU72_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU5422772.1"
                     /translation="MAGHDYQGLRERAEEFVRAAGGAVPEERLIAHVFGNGGNPALWR
                     PLLRQILDAHDRLSLRADGCWALPNARLDRDALPGDFVVLDLETTGLKPAQQRIIEVG
                     LVCFAGGAQIEAYGSLVNPERRLPAYIARLTRIDDSQLIGAPRFLQLADEILARLEGK
                     LLVGFNLGFDIGFLNAELKRLGRPALINERLDLLPLAARLLPAGRRPGLDGVCRALDI
                     EVRERHRALADAELTALAFGKLLALAREQELGSLESLQRAAAGEVPVPRRRGDVGRGR
                     AVLARDHLEGIPHTPGVYVMHDARDRVIYVGKAKDLRNRVASYYSQPLGYTRKMDGLL
                     ESIARIESVETGSELMALLVESQLIRRYQPQYNRQLRNSESYPYIKIDVGNPWPRVAL
                     TRQRADDGAVYLGPFRVASAAKTTIELIHDVFPLRSCTRSFRTARSYGSPCLELALGR
                     CPGPCVGSADREEYRATVHRVVDFLHGAQDEAIERLHDELARAADTLDFEKAARLRDR
                     LRRVQQLVLS"
BASE COUNT          715 a         1730 c         1785 g          800 t
ORIGIN      
        1 tactgccact gggcgatggt ccgattcact accccctgag cgtcggcggg acgccgctgg
       61 tcgcctcgcc ggcgctgcgc gagcggctgg gactgccgca actgtggctg aaggacgaga
      121 cgcgcggccc gaccggctcc aacaaggacc gcgccagcgc gctggtgctg gagctggcgc
      181 tgcgcgagag gcgcgatacg gtcagttgcg cctcgaccgg caacgtcgcg gtgtcgctgg
      241 cggtgggcgc ggcggcggcg ggtaggcgcg ccgtcatctt cgtcccggcg caggtctcgc
      301 cgtcaaatct gacgctgatg ctgctggcgg gcgcgacggt ggtcaaggtg cgggcgggct
      361 acaaagccgc tttccggctc tctgaggagg cggccgagcg cttcggctgg ttctcgcgca
      421 ataccggcgt caacccggtc acgctggagg ccaagaagac cgtcgcgttc gaggtctggg
      481 agcagctcaa tcgtgatctc ccggacgtgg tcgtggcacc ggtcggcgat ggcgtgacgc
      541 tggcggcgct ggccaaggga ttccgcgagc tgatcgcctg cggcgtgacc gcgcggctgc
      601 cgcgcatcat cggcgtgcag gccgagggga gcgcgccgat cgcccgcgcc tgggccaccg
      661 atcgcccact ggaggccatt gaaccgaaca cgctggccga cggcatcgcg gtcggcgcgc
      721 cggtcagtgc cggcgaggta ctgcgcgacg tgcgcgcgag cggcggcggt atggtcacgg
      781 tctcggatga gaccctgctg gactggatcg gcatcctggc gcgtgaggcc gggctgctgg
      841 ccgagccggc cggggtagcc ggcacggcgg gcgtcgccgc cgcgctggag cagggactgc
      901 tcgactggag cgagcgcgtg gtggcgctgg tgagcggcac gttgctgaag aacctgcaat
      961 tcgcccggcc cacgcgcaag ccgatcgagg tcgaggggga gctgggggaa ctggagagag
     1021 cgctggggta ggaaattccc tacttccccc cgtcgtcctc cagatcgtgc aggcgctgct
     1081 cgtcgcggcg ctcgcggagc tgttggcgca ggacgctgag ttcgtccagg cggcgctcga
     1141 tctggtcgag cgtgtcctcg cgcgggctgc gcacgtattc gtcgcgcagc tccaccagca
     1201 tctgggccag ccgggcgatg gtgcgcacca ggtgaaccgg gtcgtcctca cgggtgggca
     1261 ggctgtaggg catcagacgc ttccagtcac tgtcaccgcg cggcctcgtc ctcgccgcta
     1321 gccaggccgt tgagcatgcg cagcaactcc gcctcgccga cgatcggtac gcccagctcg
     1381 cgcgcgcgag tcgccttgct gcccggctcc tcacccacca ccaccagatc ggtcttgcgc
     1441 gtgaccgagc tggtgacgtt ggctcccgct tgccgcagta actcttccgc ctgactgcgg
     1501 gtcagcccct ccagccggcc ggtcacgacg accgtcttgc ctgccagcac cgccgaggcc
     1561 ggcggctgtg actcgaccgg ctcgctcatg cgaagcccgg catcgcgcag cgcggtgatc
     1621 atctcgacgt tgcgcggcag gtggaagaag tcatagacgg cgtcggccac gaccccgccg
     1681 atgccgggaa tggtttgcag ctcctcggta tcggccgcca tcaaccgctc catgctgccg
     1741 aagtggcgcg ccagcagctc ggcattgcgc tcaccgacgt ggcgcacgtt gaggccggcc
     1801 aggagacgcg agagcggctt ctgcttgctg gcctcgatcg aggcctgcag gttctcgatg
     1861 ctcttctcgc cgaagccctc cagctccgag atcgcgcccc agtcgaggtg gtagatatcc
     1921 ggcagccagg tgatcagacc cagccggacg aagcgctccg cggtcttgcc gccgatcccc
     1981 tcgatatcca gcgcgccgcg cgagacgaag tgcttgactt cctcctcgag ctgcgcgggg
     2041 caggaggcgt tagggcagta gcgcatcgcc tcgcccggca ggcgcacggt ctcggccccg
     2101 cagaccggac aatgcgtcgg catctcccag ggaatctcag cgccggttcg tgccggctcg
     2161 accaccgaca taatcttcgg gatcacgtcg ccggcccgct cgacgacgac cgtgtcgccc
     2221 agcatcagac ccagccggcg gatctcgtcc tcgttgtgga gcgtggcgtg gcgcaccacc
     2281 acgccgccga tctcgaccgg ctccaggatg gcgcgtgggt tgagggtgcc ggtgcggccg
     2341 acgttgacct gaatctcctt caggacggtg gtcttctgga tcgccgggaa cttgtaggcc
     2401 gtcgcccagc ggggctcgcg gccgacgatg ccgagctccc gctgcagctc gacctcgttg
     2461 accttgacca ccaccccatc gatctggaag ggaagatcgt ggcggaccga ctgccagcgc
     2521 gcgatctcgg cccagacctc gtcggtcgaa tcccagacac ggtagtcggg caccaccggc
     2581 aagccgagcg cggccagcat gtcgaggttg ccgctgtggg tcgggcgcgc ctcgccctcg
     2641 acgatgccga cgtcccaggc gaagaagcgc agcgggcggt tggcggtcac cgccgggtcg
     2701 atctggcgca gggcgccgga ggcgctgttg cgcgggttgg cgtagaggga ctcgccggca
     2761 tcgccgcgga cgcgattgag ctcctcgaag tcgcgcttgc gcatgtagac ctcgccgcgc
     2821 gcctccagca gctcgggcac cggcgtgtcg tcgatgggcc gcaggcgcag cggcaggttg
     2881 cggatggtgc ggatgttcgg cgtgacatcc tcgccctcga tgccgttgcc ccgcgtggct
     2941 ccgcgcacat acgcgccatc gcgatagagg atcgagaccg cgctgccgtc gatcttcggt
     3001 tcggtgacga attggaggtc gcggcgtccg gccagcttga agacgcgctc ggcccactcg
     3061 cgcagatcct gctcggagaa gacgttgccc aggctgagca tcggcagctc gtggcgcacg
     3121 gtgccgaagc cctgcgaggg cgtcgcgccc acgcgctggg taggcgattc cggcgtcacc
     3181 agctcggggt gctccgcctc gatgcgccgc aactcattga gcagcgcgtc gtactcagcg
     3241 tcgctgatcg aggattgatc gaggatgtag tactcgtagt tgtagcggtc gatgagcgcc
     3301 cgcagctcat ccacccgtcg tcgcaggttg gcctcgtcca tggcccgaag ctccgtcgtc
     3361 ccaccttcta gtcaccagtt gccgctggaa gtatagcacg gtttgtccgt acacttgttc
     3421 gcaccggcgc ggcccactac catccgccgc gacggcgacg tgtggggaga ggtgatggcg
     3481 gggcacgact accagggact gcgcgagcgc gccgaggagt tcgtgcgcgc ggcgggcggc
     3541 gccgtgcctg aggagcggct gatcgcgcac gtcttcggca atggcggcaa cccggcgctc
     3601 tggcggccgc tgctgcgcca gatcctggac gcccacgacc gcctgtccct gcgcgccgac
     3661 ggctgctggg cgcttcccaa cgcccgcctc gaccgtgatg ccctgcccgg cgacttcgtc
     3721 gtgctcgatc tggagacgac cggcctcaag ccggcgcagc agcgcatcat cgaggtcggg
     3781 ttggtctgct tcgccggcgg ggcacagatc gaggcctatg gcagcctggt caacccagag
     3841 cggcgcctgc cggcctacat cgcgcgcctg acccgaatcg acgacagcca gctgatcggg
     3901 gcgccgcgct tcctacagct cgcggacgag atcctggcgc ggctggaggg gaagctgctg
     3961 gtcggcttca acctcgggtt cgatatcggc ttcctcaatg ccgagctgaa gcggctgggc
     4021 cgcccggcgc tgatcaacga gcggctcgat ctcctgccgc tcgcggcgcg gttgctgccc
     4081 gccgggcgac gacccggcct cgacggcgtc tgccgcgcgc tcgacatcga ggtgcgcgag
     4141 cggcaccggg cactcgccga cgccgagctg accgcgctgg cgttcgggaa gctgctggca
     4201 ttggcgcgcg agcaggagct gggctcgctg gagtcgttgc agcgggcagc cgccggcgag
     4261 gtgccggtgc cgcggcggcg cggcgacgtc ggccgcgggc gcgcggtgct ggcgcgtgac
     4321 cacctggagg gcatcccgca cacgccgggc gtttacgtga tgcacgacgc acgggaccgg
     4381 gtgatctacg tcggcaaggc caaggacctg cgtaaccggg tcgcctccta ttactcccag
     4441 ccgctgggct acacgcgcaa gatggacggg ctgttggagt cgatcgcgcg catcgagtcg
     4501 gtcgagaccg gctctgagct gatggcgctg ctggtcgagt cgcagctaat ccgtcgctac
     4561 cagccccagt acaatcggca gctacggaat agcgagtcgt acccctacat caagatcgac
     4621 gtcggcaatc cctggccgcg tgtggcattg acccgtcaac gggccgacga cggcgcggtc
     4681 tacctggggc cgtttcgcgt ggccagcgcc gccaagacga cgatcgagct gatccacgac
     4741 gtcttcccgc tgcgcagttg cacccgctcc ttccgtaccg cccgctccta cggttcgccc
     4801 tgcctggagc tggcgctggg tcgctgccct ggcccgtgcg tcggcagcgc cgaccgcgag
     4861 gagtaccgcg ccaccgtgca ccgggtggtc gacttcctgc acggggcgca ggacgaggcg
     4921 atcgagcggc tccacgacga gcttgcccgc gcggcggaca cgctcgactt cgagaaggcc
     4981 gcccgtttgc gcgaccggct gcgccgggtg cagcagctcg tgctcagcca
//
LOCUS       DASUBI010000229         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_586379, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000229 DASUBI010000000
VERSION     DASUBI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586379"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFU72_04470"
     CDS             <1..906
                     /locus_tag="VFU72_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5422773.1"
                     /translation="VPETPGVDLTGALVGSEGMLGIVTKVMVRLTRNPEAVNVLLAAF
                     NDVESASSAVSAIVAAGLLPAALEMMDATTIKAVEAAFHAGYPEDAGAVLLIEIDGLA
                     EIVAESSDTIAQLCRQHDALDVRVARTQEERDLLWLGRKAAFGAMGRLAPNYYLQDTV
                     VPRTKLPITLKRVDEISREYQLQVANVFHAGDGNLHPLILFDRYRKGDIEKVLRAGDE
                     LLHWCVDHGGTVSGEHGIGMEKNNFLSLVFTTDDLAAMAGLKLSFDPTEMFNPDKVFP
                     SGFSCGEVASLKRQGIPYKALSDVV"
     gene            896..2149
                     /locus_tag="VFU72_04475"
     CDS             896..2149
                     /locus_tag="VFU72_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5422774.1"
                     /translation="MSSEQPETAGYVVDGVEPGRVVRPDSVAELQEVVREAAASASPL
                     IPWGAGTQMGFGNLAGPTALAVDLTRLNQIVEYEPDDLTIGIQAGATVGATNELLGQH
                     GQFLPAEAADPARATLGGVFATGIAGPRRFGFGSPRDMVLGISLVLPDGTLARGGGKV
                     VKNVSGFDLMRLNYGALGALGIIVQLNLKVLPAARAQRTVLTRYAAVTPALEAAMRVR
                     ESTLGPTAIVLVDTAASSAAGLGDAAWTLALRCEAPPAAVTRQADRIREAVSAGALDS
                     ATLDEDATLATWAALNRALSAEPAMDVVNVRFGVVPSQAAATLDACAAAAISTGWALE
                     RTADLWSGLSYARLSGPNGMLLGAWRALAHAGSHRSLLSAPAAVKAETDVFGARNEGF
                     PLMEALKEQFDRARIFNRGRFIGKL"
     gene            2163..>2809
                     /locus_tag="VFU72_04480"
     CDS             2163..>2809
                     /locus_tag="VFU72_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5422775.1"
                     /translation="MVLEIEKLVEQASNFSGPDAPSFDVIRSCVHCGLCLPACPTYRL
                     TGRERSSPRGRIWLMKQVATGQVELADPIFAEEMALCLNCRACEAVCPSGVRYGQILE
                     ASRAQLEENLPTTVKKRVLRAAGFDLLFRDMRVFRTVNRGLKLYQRTPLRAAARKSGV
                     LRVLGMDDAERLMPALSDDFVVPHGETYPALGERRGRVGLFTGCVMSTAYAPVHQA"
BASE COUNT          445 a          928 c          990 g          446 t
ORIGIN      
        1 gtgccggaga cgccaggggt cgatctgacc ggcgcgctgg tcggctccga agggatgctc
       61 ggcatcgtga ccaaggtcat ggtgcggctg acgcgcaacc ccgaagcggt caatgtgttg
      121 ctcgccgctt tcaacgacgt cgagagcgcc tcaagtgcgg tctcggccat cgtggcggcc
      181 gggctgctgc cggcggcatt ggagatgatg gacgccacca cgatcaaggc agtcgaggcg
      241 gcgttccacg ccggttaccc cgaagatgcc ggggcggtcc tgttgatcga gatcgacggg
      301 ctggccgaga tcgtggccga gagcagcgac acgatcgcgc agctctgccg ccagcacgac
      361 gcgctcgatg tgcgcgtagc acgcacgcag gaggagcgcg atctgctctg gttgggccgt
      421 aaagccgcct tcggcgcgat gggccgactg gcccccaact actacctgca agacacggtg
      481 gtgccgcgca cgaagctgcc gattacgttg aagcgcgtgg acgagatcag ccgcgagtat
      541 cagctccagg tcgccaatgt ctttcacgcg ggcgacggca acctgcaccc gctgatcctc
      601 ttcgaccgct accgtaaggg cgacatcgag aaggtgctgc gggcgggcga cgagctgctg
      661 cactggtgcg tcgaccacgg cggcacggtc tcgggcgagc acggcatcgg catggagaag
      721 aataatttcc tgtcgctcgt cttcaccacc gacgacctgg cggccatggc cgggctcaag
      781 ctgagctttg acccaaccga gatgttcaac cccgacaagg tcttcccatc cggcttctcc
      841 tgcggtgagg tagccagcct caagcgacag ggaatcccct acaaggcgct gagcgatgtc
      901 gtctgagcag ccggagacag ccggctacgt ggtcgatggc gtcgaacccg ggcgagtcgt
      961 gcggccggac tcggtcgcgg agttgcagga ggtcgtgcgt gaggcggcgg cttcggcgtc
     1021 accgctcatc ccctggggcg ccggtacgca gatgggtttc ggcaatctgg cgggaccgac
     1081 cgcgctggcg gtcgatctga cccgactgaa ccagatcgtc gaatatgagc ctgacgatct
     1141 caccatcggc atccaggccg gagccaccgt cggtgccacc aacgagctgc tgggccagca
     1201 cgggcagttc ctgcccgccg aggcagccga tccggcgcgg gccacgctgg gcggggtctt
     1261 cgcgaccggg atcgccggac cgcgccgctt cggctttggc tcgccgcgcg acatggtcct
     1321 cggcatcagc ctggtgctgc cggacggtac gctggcgcgc ggcggcggca aggtggttaa
     1381 gaacgtctcc ggcttcgatc tcatgcgcct caactacggt gcgctcggcg cgctggggat
     1441 catcgtgcaa ctcaatctca aggtactgcc cgcggcccgc gcgcagcgga cggtgctgac
     1501 ccggtacgcc gctgtgacgc cggcactgga ggcggccatg cgcgtgcgcg agtcgacgct
     1561 cggcccgacg gccatcgtac tggtcgacac ggcggcaagc agcgcggccg ggctcggtga
     1621 cgccgcctgg acgctggcgc tgcgctgcga ggcccctccg gcggcagtca cgcgccaggc
     1681 agatcgaatt cgcgaggcgg tctcagccgg cgcgctcgac agtgcgacgc tagacgaaga
     1741 cgcaacgctc gctacctggg cggcgctcaa ccgggcactg tcggccgaac cggcaatgga
     1801 cgtcgtcaac gtgcgctttg gtgtggtccc ctcgcaggcg gccgcgacgc ttgacgcgtg
     1861 cgccgcggcg gcgatttcaa cgggatgggc cttggagcgc acggccgatc tgtggagtgg
     1921 gctgagctac gcgcgcctaa gcggaccgaa cgggatgttg ctgggcgcct ggcgggcgct
     1981 ggcgcacgcc ggcagccacc gcagcctgct cagcgcgccg gcggcggtca aggcggagac
     2041 cgacgtcttc ggcgcgcgca acgagggctt cccactcatg gaggcgctca aggagcaatt
     2101 cgaccgggcg cgcatcttca accgtggccg cttcatcggc aagctctaga aggtggtgga
     2161 cgatggtcct ggaaatcgag aagctggtcg agcaggcgag caacttcagc ggcccggacg
     2221 cgccgagctt cgacgtgatc cgctcgtgcg tccactgcgg cctctgtctg ccggcctgcc
     2281 ccacctaccg cctcaccggg cgtgagcgct cgtcgccgcg cgggcgcatc tggctgatga
     2341 agcaggtcgc caccggccag gtggagctgg ctgatccgat ctttgcggag gagatggcgc
     2401 tctgcctgaa ctgtcgcgcc tgcgaggcgg tctgcccttc gggcgtgcgg tatgggcaga
     2461 ttctggaagc atcgcgtgcc cagcttgagg agaatctgcc caccacagtg aagaagcgtg
     2521 tgctccgtgc ggccggcttc gatctcctct tcagggacat gcgcgtcttc cgcacggtca
     2581 accgcgggtt aaagctctat cagcgcacgc cgctacgcgc ggccgcacgc aagagcggcg
     2641 tactgcgcgt gctcggcatg gacgatgccg agcggctgat gccggcgctt agcgacgact
     2701 tcgtggtgcc ccacggcgag acttaccccg ccctgggcga gcggcgcggg cgagtcggcc
     2761 tcttcaccgg ctgcgtgatg agcaccgcct acgcgccggt ccaccaggc
//
LOCUS       DASUBI010000230         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_207936, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000230 DASUBI010000000
VERSION     DASUBI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207936"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFU72_04485"
     CDS             <1..309
                     /locus_tag="VFU72_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU5422776.1"
                     /translation="DYFLLLPGQAIHGVTRERVTLPDNICGWIQGRSRFARVGLMVHV
                     TASFMQPGIVDASQVLEMNNAGPMPLAIHPGTAICQIILEECVGSAHYSGRFQSQRLP
                     "
     gene            609..1901
                     /locus_tag="VFU72_04490"
     CDS             609..1901
                     /locus_tag="VFU72_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422777.1"
                     /translation="MMDVADLRHDEIAANAGPFTILSAVAWPLAGLSLALIVGSLLLA
                     TRNQLNLTDMGHLPFVASAALVGGVLNRRRPANLVGWLFTGTGLSFALLEFTGQYASY
                     GLITHPGALPFARALAWPQSWLWVPGGLLLFAFLPLLFPQGRLITPGWRWVLRANIAI
                     CLVLVVLSAVLPGGMGNGSVGGGRVQNPLGIAALSGMMGTLNLIGPLLVLVIVVTATA
                     SLVTRFRHSRGLERQQMKWLTFAVVLYPVAMAVNHWVAAVPLLVPLTLIAVPLAAGLA
                     IFRYRLYDIDWLIDKTLVYIPLTAILAGLYSASTTLCQRVFVSATGETSGAAIVLTTL
                     TLAAAFTPIKNSLQAVVDRRYKPARQPLVVAEGGVPVAQGAEERAVDDEGLRALLLAQ
                     QEVLTDLARRLDRLEGARAATSNGQASQAAASSSRQAP"
     gene            2080..3048
                     /locus_tag="VFU72_04495"
     CDS             2080..3048
                     /locus_tag="VFU72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU5422778.1"
                     /translation="MAAQVLLVAVAVAFAWSMGAHYTGATMGMPYATRSIQLWPALGT
                     IALLALIGATFASHKVERTVGHGILGTSSISISLGAATIIIATAFALTTMFNYLRIPT
                     STIQILVFCVIGVGLAVHLSIQWHTILRLVIIWVLAPVVAVGLGFGFTRIIDLLAPGT
                     KGTIGSFGPWHAVLVVVGAGASFAMGANDVSNATGAFLMTHLFDVFVAGLIGGIGLSV
                     GALTWGKPLLERVAFDIVKMDLAMASAAQFVQALVVLTAVAFGYFTSMNQALVGAMAG
                     TGLARGRDTVQWPVVRGILKGWAFGPASGLAIAYVAARLIGLVVHL"
     gene            3221..>4521
                     /locus_tag="VFU72_04500"
     CDS             3221..>4521
                     /locus_tag="VFU72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEU5422779.1"
                     /translation="MLERDEILTIYAQGPEAVVALVQALTAELTTVQTRLQALEARLA
                     LDSHNSSQPPSRDEPSTPAPKSLRQRSGRKPGGQPGHPGTTLAWSETPEVVVRHRPTT
                     CAGCQAPLALMPVVGVERRQVLDLPELRLVTTEHVVEACRCAACGHLTRGRFPAAARA
                     ALGYGPRLRSVAVALHQYQLLPSARVAAFLGDLFGCSFAPGTLATAVADCHTALAASE
                     TAMQQALRQAAVLHVDETGLRVDGGRQWLHVASTDRLTYYALQPGRRADLIAACGVLS
                     AFRGVAVHDGQAAYFTFPCDHALCNVHHLRELTFVAEQLQQGWAAQLKTLLLEMKAAV
                     DAARAAGERHLGWVATVKFELRSWQLVRQGLAANPPQEKPPNRPGPPKRTKGGKLALR
                     LARDAEAVLRFLHDFTVPFDNNQAERDLRMVKVQQKIAGCFR"
BASE COUNT          617 a         1586 c         1582 g          736 t
ORIGIN      
        1 gactacttcc tcctgctgcc ggggcaggcg atccacgggg tgacgcgcga gcgcgtcacg
       61 ctgccggata acatctgcgg ctggatccaa gggcgcagcc gcttcgcgcg ggtcggtctg
      121 atggtgcacg tcaccgccag ctttatgcag ccggggatcg tcgacgccag ccaggtgctg
      181 gagatgaaca acgccgggcc gatgccgctg gcgatccacc caggcaccgc catctgccag
      241 atcatcctcg aggagtgcgt cggcagcgcg cactacagcg gccgctttca gtcgcagcgc
      301 ttgccttgag tagtcgagga cgcgctctgt tcctccactc gcacagttcg gcgcgctcgc
      361 gcctggcgta agcgctcctg cggcgacaga ccaatggtcg tcggaattgg cgtgtgccgg
      421 cgcgcacgct agaattaatg gtcgaagcgg taatcaagtg tcccggcgct cgcgttcgag
      481 accgccggcg ggttcgagcg cgatcggcgc gcgccctgct gccgccccgt ggccatacgg
      541 ctggctacct cgcgatgcct ggcgccacgc cgctcccccc cgtccgagcg gcgcgaggag
      601 aggaccgaat gatggacgtg gctgacctgc ggcacgacga gatcgccgcc aacgcggggc
      661 cgttcacaat cctgagcgcc gtcgcctggc cgctagccgg gttgagcctg gcgctgatcg
      721 taggaagcct gctactggcg acgcggaacc agctgaacct cacggatatg gggcacctgc
      781 cgttcgtagc cagcgcggcc ctggtcggcg gggtgctgaa ccggcgccgt ccggctaacc
      841 tggtcggctg gctcttcacc ggcaccggcc tgagcttcgc gctcctcgag ttcaccgggc
      901 agtatgcctc atacggcctg atcacccatc caggggcgct ccccttcgcg cgggcgctgg
      961 cctggccgca gtcatggctg tgggttccgg gcggcttgct cctcttcgcc tttctgccgc
     1021 tcctctttcc gcagggccgc ctgatcacgc ccggctggcg ctgggtcctc cgcgccaaca
     1081 tcgcgatctg cctcgtcctc gtcgtcctgt cggccgtgct gcccggcggc atgggcaatg
     1141 gctcggtcgg cggcggacgc gtgcagaacc cgctcggcat cgctgcgctc agcgggatga
     1201 tgggcaccct caacttgatc gggccgctcc tggtgctggt catcgtggtc acggcgaccg
     1261 ccagcctggt cacgcgcttc cgccactccc gagggctcga gcggcagcag atgaaatggc
     1321 tcaccttcgc cgtggtgctg tacccggtcg ccatggccgt caaccactgg gtggcggccg
     1381 tgccgctgct ggtgccgcta acgctgatcg ccgtcccgct ggcggcgggg ctcgccatct
     1441 tccgctaccg tctgtacgac atcgactggc tgatcgacaa gaccctcgtc tacatccccc
     1501 tgaccgccat cctcgccgga ctctacagcg cgtccaccac gctctgccag cgcgtgttcg
     1561 tctcggcgac cggcgagacc tccggcgcgg cgatcgtcct gacgaccctc accctggccg
     1621 cggccttcac gccgatcaag aacagcctgc aagcggtagt ggaccggcgc tacaagcctg
     1681 cccgacagcc cctcgtcgtc gccgaaggcg gcgtccccgt cgcgcagggg gcggaggagc
     1741 gcgcggtcga cgacgagggg ctgcgcgcgc tgctgctggc gcagcaagaa gtcctgacgg
     1801 acctggctcg ccggctggat cggctggagg gcgcccgggc ggccacctcc aacgggcagg
     1861 cgagccaggc agcggcgtcc tcttcccgcc aagctccctg actcagcgga gccgcaacct
     1921 ccatggcccc acgcagaacc ggccgcgggt aggctatgcg gcggttccgg cgccagaccg
     1981 attccgatcg cccgccgcga gaaatccacg cccgcgtgct accatgcagg cgccgcaacg
     2041 gtgtacgtac ctgtgcggca ggcgtggaaa gggaaggtga tggcggcaca ggtcctcctc
     2101 gtcgcggtgg cggtggcctt cgcctggagc atgggcgcgc actacaccgg cgccaccatg
     2161 ggcatgccct acgcgacccg ctcgatccag ctctggccgg cgctcggcac catcgcgcta
     2221 ctggcgctga tcggcgccac cttcgccagc cacaaggtcg agcgtaccgt cgggcacggc
     2281 atcctgggca cctcgtcgat ctcgatcagt ctgggcgcgg ccaccatcat catcgccacc
     2341 gccttcgccc tgacgaccat gttcaactac ctgcgtatcc ccacctcgac cattcagatc
     2401 ctggtcttct gcgtcatcgg cgtcgggctg gcggtgcacc tgtcgatcca gtggcacacc
     2461 atcttgcgcc tggtcatcat ctgggtgctg gctcctgtgg tggcggtcgg cctcggcttc
     2521 ggcttcaccc ggatcatcga cctgctggcc ccgggcacca aagggacgat cggctccttc
     2581 ggcccctggc acgcggtgct ggtggtcgtg ggcgccggcg cctccttcgc catgggcgcc
     2641 aacgacgtct ccaacgccac cggcgccttc ctgatgaccc acctcttcga cgtcttcgtg
     2701 gccgggctga tcggcggcat cgggctgagc gtgggcgcgc tgacctgggg caagccgctc
     2761 ctggagcggg tggccttcga catcgtcaag atggatctgg cgatggccag cgccgcgcag
     2821 ttcgtgcagg cgctggtggt gttgaccgcg gtggccttcg gctacttcac gtcgatgaat
     2881 caggcgctgg tcggcgccat ggccggcacc gggctcgccc gcgggcgcga cacggtgcag
     2941 tggccggtgg tgcgcggcat cctcaagggc tgggccttcg gcccggcctc ggggctggcg
     3001 atcgcctacg tcgccgcgcg cctgatcggc ctggtggtgc atctgtaggt ccattccgag
     3061 gaatcgcatg gctgcgccga accgcggcag cgtgggaact cggcggtggc cggcgaggag
     3121 catgggtgct ggtcgaggac ggatccttcg actgcgcgta actgttcagg tcgggggtca
     3181 ggggcttgtc aagcagcgtg ggtacgggta ggctgcgcgc atgctggaac gcgacgagat
     3241 tctgaccatc tacgcgcaag gaccggaagc cgtggtggcg ctggtgcagg cgctcacggc
     3301 ggagttgacg accgtccaga cccgcctgca ggcgctggag gcgcgactgg cgctcgactc
     3361 gcacaactcc tcccagccgc cgtcgcgcga tgaaccgagc acgcccgcgc ccaaaagcct
     3421 gcggcagcgg agcggacgga aaccgggcgg gcagccgggg catccgggga cgacgttggc
     3481 ctggagcgag acgccggagg tggtggtgcg gcaccggccg acgacctgcg ccggttgtca
     3541 ggcgcccttg gccctgatgc cggtggtcgg ggtcgagcgg cggcaggtgc tggacctgcc
     3601 ggagttgcgg ctggtgacga ccgagcacgt ggtggaggcc tgccgctgtg cggcctgcgg
     3661 ccacctcacc cggggcaggt tcccggctgc ggcgcgggcc gcgctgggct atggtccccg
     3721 gctgcggagt gtggcggtcg ccctccacca gtatcagctc ctgccctccg cgcgcgtcgc
     3781 ggcgttcctg ggcgacctct tcggctgctc cttcgcgccg ggcacgctgg cgacggccgt
     3841 ggccgactgt cacacggcgc tggcggcgtc cgagacggcc atgcagcagg cgctgcggca
     3901 ggccgcggtg ctccatgtgg acgagaccgg gctgcgggtg gacgggggac ggcagtggct
     3961 gcatgtggcc agcaccgacc ggttgacgta ctacgcgctg cagccgggcc gccgggcgga
     4021 cctcatcgcc gcgtgcgggg tgctctccgc ctttcggggg gtggcggtgc atgatgggca
     4081 ggccgcctac ttcaccttcc cctgcgacca cgcgctctgc aacgtgcatc acctgcgcga
     4141 gctgaccttc gtggccgagc agctgcagca aggctgggcc gcccagctca agacccttct
     4201 cctggagatg aaagcggcgg tggacgcggc gcgggcggcg ggggagcgcc acctcggctg
     4261 ggtggccacg gtcaaattcg aactgcgctc ctggcagctc gtgcgccagg ggctggcggc
     4321 gaacccgccg caggagaaac cccccaatcg acccggtccg cccaagcgga cgaaaggggg
     4381 caagctggcg ctgcggctgg cgcgggatgc tgaggccgtc ttgcgcttcc tgcacgactt
     4441 caccgtgccc ttcgacaaca accaggccga gcgcgatctg cgcatggtca aggtgcagca
     4501 gaagatcgcc ggctgcttcc g
//
LOCUS       DASUBI010000231         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_44495, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000231 DASUBI010000000
VERSION     DASUBI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44495"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1065)
                     /gene="hpt"
                     /locus_tag="VFU72_04505"
     CDS             complement(112..1065)
                     /gene="hpt"
                     /locus_tag="VFU72_04505"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5422780.1"
                     /translation="MTETDRVDARTEARTDARPVPPHSFAHPAEAEFAAFLDFYRIRW
                     MYEPKSFPLRWRDDRPAEMFTPDFYLPEQDLYIELTTMKQSLVTRKNRKVRRLRQLYP
                     NINITLLYRKDYHELLSRFGYGAVDITSLKAEQIERVLLSAAEIQERVAELGAEISAD
                     YAGQSIVLVGVLKGITFFLADLARAITRPLAIDFLQLSHSVERGVALGQDLDIDIHGQ
                     HVLLVEDIVNTGVSLDFVLRELRERQPASLKVCALFDKDERRLLPVPVDYVGFTIPNE
                     FVVGYGLDYRQLYRNLPFICVLKRSVYEADTPALAGMSAGR"
     gene            complement(1137..1682)
                     /locus_tag="VFU72_04510"
     CDS             complement(1137..1682)
                     /locus_tag="VFU72_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU5422781.1"
                     /translation="MYWNRKHVLICTASHCAQKGAQQVAGRLRIELKRKGLDVDVLAN
                     TCDSIDLCDIGPNIVVYPEGTIYHGVTVKDIPDLIASLREGGQPVERLVLEPHAGAEE
                     ERRRFYHEANAVEPLTPEAFAEIAARYGFDANWVNEQARRGFIARKEVDGQPVITIIS
                     KARNRYGLPAAAPAQAGIQDR"
     gene            complement(1769..3328)
                     /locus_tag="VFU72_04515"
     CDS             complement(1769..3328)
                     /locus_tag="VFU72_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU5422782.1"
                     /translation="MTAANRPDETTGARPPASGAELPFRSVVLIQPSRQRMAELQARE
                     GLPASLAEHLQDLRVAPGTLRADGWLYVRVNVPTRRAGERIFWERVDLILGAECLILI
                     QPRPLTVVRRALARAQRRAQTPADLLALVLSQVVESFHLLFWSIERELAGLEPDEGGP
                     APATAELRAQVGELDRADDSLRGVMQEILDSDALPADSPAANELHTSLDRLTTIEADT
                     DRIEEQLDELKAQRGEEEAEARVLGAEAPEVFASATRAGERRLERLNLAHAVTALVGG
                     LAVSFGAVAMVWVGGPWEASLGEGRAHLLGALAFPIGFVILLIGKGELFTEDFFVPVT
                     GVIRGRGRVRDLLTLWGYVLFFNFVGALIFAFLLSRAGVLEPAPRQFLIDLAVAKVHY
                     SFELALMRGIFAGWLMTIMTWLILAADGFGARLVIIWMTGFLIVAGHFNHVVISMPEV
                     FTAMFLGAPITIPQWLVRNFVPALLGNLIGGVVFVTLLGYAQARSLEMSEEQRQANGN
                     GNGNGNGNGNG"
     gene            complement(3427..>3718)
                     /locus_tag="VFU72_04520"
     CDS             complement(3427..>3718)
                     /locus_tag="VFU72_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422783.1"
                     /translation="RDLIEAAAAFTAILDEPTGPTVEEDERERVVRFAAGEQVLTLAE
                     PLVETGALAERIQRKGDGPFEGRLAARGIEQARLIPMNQADGARLRLIPTGA"
BASE COUNT          580 a         1267 c         1314 g          557 t
ORIGIN      
        1 acctgtcatt gtgagccgca gcgaaggatc tctcatcggc ttggagtggc agcagccgcg
       61 cacggtagcg gagattctgc cgcaagctcg gggcgaacga gtggaaggcg gctagcgccc
      121 ggctgacatc cccgccaacg cgggcgtgtc ggcctcgtag acggagcgct tgaggacgca
      181 gatgaagggg aggttgcggt agagctggcg gtagtccagc ccgtagccga ccacgaactc
      241 gttggggatg gtgaagccga cgtagtcgac cggcaccggc agcagccgcc gctcgtcttt
      301 atcgaacagg gcgcagacct tcaggctggc cggctggcgc tcgcgcaact cgcgcagcac
      361 gaagtcgagc gagacgccag tgttgacgat gtcctcgacc agcagcacgt gctgcccgtg
      421 gatgtcgatg tcgagatcct ggcccagcgc caccccgcgc tcgacgctgt gcgacagctg
      481 caggaagtcg atcgccagcg ggcgggtgat ggcgcgcgcc aaatcggcca ggaagaaggt
      541 gatccccttg agcaccccga ccagcacgat cgactgaccg gcatagtcgg ccgagatctc
      601 ggcgcccagc tcggccaccc gctcctggat ctcggccgcg ctgagcagca cgcgctcgat
      661 ctgctccgcc ttgagcgagg tgatgtcgac cgcgccgtag ccgaagcgcg acaggagctc
      721 gtggtagtcc ttgcgataga ggagcgtgat gttgatgttg gggtagagct ggcgcaggcg
      781 gcgcaccttg cggttcttgc gcgtcaccag gctctgcttc atggtcgtca gctcgatgta
      841 gagatcctgc tccggcaggt agaagtcggg ggtgaacatc tcggccgggc ggtcgtcgcg
      901 ccagcggagg ggaaaggact tcggctcgta catccagcga atgcggtaga aatcgaggaa
      961 ggcggcgaac tcggcttcgg cggggtgagc gaaggagtgc ggcggaaccg ggcgggcgtc
     1021 cgtccgcgct tcggtcctgg cgtccacgcg gtcagtctcg gtcatccgac ctcctcagca
     1081 ccgacagcgc cactttcact tcgactatag cgaactttcc tcgcttgcgc aagtgtctag
     1141 cggtcctgga tcccggcctg cgccggcgcc gcagcgggca ggccgtagcg gttgcgcgcc
     1201 ttgctgatga tcgtgatcac cggctggccg tcgacctcct tgcgcgcgat gaagccgcgc
     1261 cgcgcctgct cgttgaccca gttggcgtcg aagccgtagc gcgcggcgat ctcggcgaag
     1321 gcctccggcg tcagcggctc gacggcgttc gcctcatggt agaagcggcg gcgctcctcc
     1381 tcggcgcccg cgtgcggctc gagcaccagc cgctccaccg gctgcccgcc ctcgcgcagg
     1441 gacgcgatca gatccgggat gtccttgacc gtgacgccgt ggtagatcgt gccttcgggg
     1501 tagacgacga tgttcggccc gatgtcgcag aggtcgatcg agtcgcaggt gttggccagc
     1561 acgtcgacgt cgaggccctt gcgcttcagc tcgatgcgca gccggccggc gacctgctgc
     1621 gcccccttct gggcgcagtg cgacgcggta cagatcagaa catgcttgcg attccagtac
     1681 atctggtctc cggactagga gcgagccggc ggcccgtggg gccgcggccg gactttccat
     1741 accctgaagt ttaccaccgc cttcccgctc atccgttccc attgccattg ccgttcccgt
     1801 tgccgttgcc gttcgcctgg cgctgctcct cgctcatctc gagcgagcgc gcctgggcgt
     1861 agccgagcag ggtgacgaag acgacgccgc cgatcaggtt gccgagcagc gccgggacga
     1921 agttgcgcac cagccactgt gggatggtga tcggcgcgcc caggaacatg gcggtgaaga
     1981 cctcgggcat cgagatcacc acgtggttga agtggcccgc cacgatcagg aagccggtca
     2041 tccagatgat gaccaggcgg gcgccgaagc cgtcggcggc caggatcagc caggtcatga
     2101 tcgtcatcaa ccagccggcg aagatgccgc gcatcagcgc caactcgaag gagtagtgga
     2161 ctttcgccac ggccaggtcg atcaggaact ggcgcggcgc cggctccagc acgccggcgc
     2221 ggctgagcag gaaggcgaag atcagcgcgc ccacgaagtt gaagaagagg acgtagcccc
     2281 agagcgtcag cagatcgcgc acgcggccac gaccgcgaat cacgccggtg accggcacga
     2341 agaagtcttc ggtaaagagc tcgcccttgc cgatcagcag aatgacgaag ccgatcggga
     2401 aggccagcgc ccccagcagg tgggcgcgac cctcgcccag cgacgcctcc caggggccgc
     2461 caacccagac catcgccacc gcgccgaagc tgaccgccag gccgccgacc agggcggtca
     2521 cggcgtgcgc caggttgagg cgctccagcc tccgctcgcc ggcgcgggtg gcgctggcga
     2581 agacctcggg cgcctcggcg ccgagcaccc gcgcctcggc ctcctcttca ccccgctgcg
     2641 ctttcagctc gtcgagctgc tcctcgatgc ggtcggtgtc ggcctcgatg gtcgtcaggc
     2701 ggtcgagcga ggtgtgcagc tcgttggcgg cgggcgagtc ggccggcagc gcgtcactgt
     2761 cgaggatctc ttgcatgacg ccgcgcaggc tgtcgtcggc gcggtccagc tcaccgacct
     2821 gggcgcgcag ctcggccgtg gccggcgccg gtccgccctc gtccggctcc agcccggcca
     2881 gctcgcgctc gatcgaccag aagaggagat gaaagctctc gaccacctgg ctcagcacca
     2941 gggccagcag atcggcgggt gtctgcgccc ggcgctgggc gcgggccagc gcccggcgca
     3001 cgaccgtcag cgggcgcggc tggatcagga tgaggcactc tgcgccgaga atgaggtcga
     3061 cgcgctccca gaagatgcgc tcgccggcgc gacgggtggg cacgttgacc cgcacgtaga
     3121 gccagccatc ggcgcgcagc gtgcccggcg cgacgcgcag atcctgcagg tgctcggcca
     3181 gcgaggcggg cagcccctcg cgcgcttgca gctcggccat ccgctggcgc gagggctgga
     3241 tcagcacgac agagcggaac ggcagctcag cgccggaggc cggcggccgt gcgccggttg
     3301 tctcgtccgg ccggttggcg gcggtcatcc tggatcctct ctcgacccgc ccgaccggcg
     3361 gatgctgcgg cggcgcgacg gcgtgtcgcg cccgctaacg cagggagagt gccaggtgac
     3421 gcgccctcag gcgccggtgg ggatcaagcg cagccgggcg ccgtcggcct ggttcatcgg
     3481 gatcagacgc gcctgctcga tcccgcgcgc ggccaggcgg ccctcgaagg ggccgtcgcc
     3541 cttgcgctgg atgcgctcgg ccagcgcacc ggtctccacc agcggctcgg ccagcgtgag
     3601 cacctgctcg ccggccgcga agcgcaccac ccgctcgcgc tcgtcctcct cgacggtggg
     3661 gccggtcggc tcatcgagga tggccgtgaa ggccgcggcg gcctcgatca ggtcgcgc
//
LOCUS       DASUBI010000232         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_185269, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000232 DASUBI010000000
VERSION     DASUBI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185269"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFU72_04525"
     CDS             complement(<1..1181)
                     /locus_tag="VFU72_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5422784.1"
                     /translation="MPFQARHIDPAGSAQARLGAELRRLRAARKLSQAGLGQLIQYSP
                     DLIRRVETTERFPAREFVEACDKALSADGALLALWPAADQERHSATPNRSVASALPPP
                     RFDPDTSAPLVEHWLAARGDHADFSCDAADRLWMTAADVELAEDTMRMFRQLDHAHGA
                     GNFAAHLKLYIDNELDPLMRRPSASPDVAVARARVATGFYELAGYQAVDCGRPGWAQF
                     YYGRALALTAQAADRGYGSYLVAANLAHLALHCDHPAIALAWASGARAEADRSASPVT
                     RAAVIAVVARAHARLGEERETTRLLVQAEELLDRADADDEPPWIRYFNHGYLADEMAH
                     CFHDLGRPPAARVQVADALDGVGRSHVRRLAIDAALLASTWLRSGELDEACAVGRQAV
                     D"
     gene            complement(1295..>2023)
                     /locus_tag="VFU72_04530"
     CDS             complement(1295..>2023)
                     /locus_tag="VFU72_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422785.1"
                     /translation="RSIGARTDRGTWVRIEARRPEKIDGQGWNGTECAALLEGIAKPD
                     WYAAFSWRDAERAVMWRADETSLVTAAPVKPGGILTSEPTLSEDWWASLDASLTALAA
                     APTTRVSTPDTEPMTQHRLTETIERVFPGLTLTQVDEWTTAHADLNWANLTAPECFLL
                     DWEDWGRAPRGLDAANLWVNSLAVPALARRVREQRSADLASRSGRLTALFYCAQIVVA
                     ADESDPLFAPAQREANSLLEDLAE"
BASE COUNT          314 a          682 c          684 g          343 t
ORIGIN      
        1 taatccaccg cttgacggcc gaccgcgcac gcctcgtcca gctcgcccga acgcagccat
       61 gtactcgcca agagcgcggc gtcgatcgcg agccgacgaa cgtgcgaacg ccccacgccg
      121 tcgagcgcgt cggccacctg aacacgggcc gccggtggac ggccgaggtc gtggaaacag
      181 tgcgccatct cgtcggcgag atacccgtgg ttgaagtatc tgatccatgg cggctcgtcg
      241 tcggcgtcgg cgcggtcgag caactcctcg gcctgaacca ggagtctggt ggtttcgcgc
      301 tcttcaccga ggcgagcatg tgcccgagcg acaactgcga tgaccgcggc gcgcgtgacg
      361 gggctggctg accggtccgc ctcggcacgt gctccgctcg cccacgccaa ggcgatggcc
      421 ggatggtcac aatgcagcgc cagatgcgca agattcgccg caacgaggta gctcccgtag
      481 ccgcgatccg cagcttgcgc tgtcaacgcc aacgcccgac cgtagtagaa ctgcgcccac
      541 cccggccgac cgcagtccac ggcctggtaa cccgccagct cgtagaaccc tgtcgccaca
      601 cgcgcacgcg ccaccgcaac atcaggactg gccgacggcc ggcgcatgag cgggtcaagt
      661 tcattgtcga tgtagagctt cagatgcgcc gcgaagttcc cggcgccgtg tgcatggtcc
      721 agctggcgaa acatccgcat cgtgtcctct gccaactcca cgtcggcggc ggtcatccac
      781 agtctgtcgg ctgcgtcgca cgagaagtcc gcgtggtctc cacgggcggc tagccaatgc
      841 tcgaccagcg gcgccgacgt gtcggggtca aaccgcggag gtggcagcgc actggcaacg
      901 cttcgattcg gcgtcgcact gtgacgctcc tggtcggcag ccggccacag cgcgagcagg
      961 gcgccgtccg cgctaagcgc cttgtcgcac gcctcgacga actcgcgtgc cggaaaccgt
     1021 tccgttgtct cgacccggcg gattaggtca gggctgtact gaatcagctg gcccaggcct
     1081 gcttggctca gcttgcgcgc ggcccggagt ctgcgcagtt cggcgccgag cctggcctgg
     1141 gcagacccgg ccggatcgat gtgccgcgct tggaagggca tcccgtttcc ctgcacacgt
     1201 gggtgacgct ccgactttgt cggagcgtca ctttaccgtc acggtgcgct gatcgcagta
     1261 ttttgtgaat cgcgatcagt tctcgaacag ggcgctattc agcgagatcc tcaagcaggc
     1321 tgtttgcttc gcgctgggct ggagcgaaga gcgggtctga ttcgtctgcg gccacgacga
     1381 tctgggcgca gtagaaaagg gcggtcagtc ggccggatct gctggccagg tccgcgcttc
     1441 gttgctcgcg gacgcgtcgg gccagtgcgg gcacggcgag ggagttgacc cagaggttcg
     1501 ccgcatccaa gccgcgcgga gctcgacccc agtcctccca atccagcagg aagcactccg
     1561 gagcggtgag gttggcccag ttcagatccg cgtgggcggt ggtccactcg tcgacctgcg
     1621 tgagggtgag gccggggaag acgcgctcga tcgtctctgt tagacggtgc tgggtcatcg
     1681 gctcggtgtc gggcgtggat acgcgcgtcg taggggcggc ggccaacgca gtcagcgacg
     1741 cgtcgagtga cgcccaccag tcctcggaca gtgtcggctc gctcgtcaga ataccgccgg
     1801 gtttgaccgg cgcggcggtg accaggctgg tctcgtccgc gcgccacatc accgcgcgtt
     1861 cggcgtcgcg ccaggagaat gcggcgtacc agtccggttt ggcgatgcct tcgagtagcg
     1921 ctgcgcattc cgtgccgttc cagccctggc cgtcgatctt ctcgggcctg cgcgcctcga
     1981 tccgcaccca cgtgccgcgg tcggtgcgcg cgccgatcga gcg
//
LOCUS       DASUBI010000233         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_650433, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000233 DASUBI010000000
VERSION     DASUBI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650433"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFU72_04535"
     CDS             complement(<1..1166)
                     /locus_tag="VFU72_04535"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5422786.1"
                     /translation="MTKEHFETLAIHAGQEPDETTGAVIVPIYQTSTFAQEEVGKHKG
                     FEYSRTDNPTRSALQTALAALEDGRWGLTYASGLAATQNAWYLLSPGDHLIMSDDAYG
                     GTYRLASKVWSRYGIDYSMVDLTNLDAVRAAIRPSTRMIWVETPTNPYLKIVDIAGIA
                     QVKAGKPIRLVVDNTFASPYLQQPLVLGADMVLHSTTKYLGGHSDVVGGAILGNDEEI
                     YDTLKFHQNAAGAVPGPFDAWLVLRGIKTLAVRMRQHSSNGEAIAAMLLEHPAVETVY
                     YPGLPGHANHDVAARQMPNGFGGMVSFTVRAGYDAARAVVAGTRLFTLAESLGGVESL
                     IEHPGQMTHASMAGSGFEVAPNLIRLSVGIEHAGDLIDDLRQALKVAVPAGVAGS"
     gene            complement(1166..>2185)
                     /locus_tag="VFU72_04540"
     CDS             complement(1166..>2185)
                     /locus_tag="VFU72_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5422787.1"
                     /translation="DRLTREIPNAFQPNQYFNPMNPRSHYLTTGPEIWQQTAGRVTHF
                     VAGVGTGGTISGIGKYLKEQNPTVQVIGADPAGSIYTDPDNVRPYKVEGIGEDFWPGT
                     FDRSLVDEFVQVGDRDSLLSARRVTREEGILVGGSGGTAVWAALEIARREDQPETLVV
                     VILPDSGRGYLSKVYNDDWMRENGFLTRFGQATRVGTLIGQQRAGVGVAPELPSVVGV
                     SVEETVEAAIDLLHRYRISQMPVLRQGASAGRIDVHDVVGSIQERTLLDQVFRTPEAV
                     HQQVTSVMDPPFALVNVKEEVERVVPHLSAGSPAVLVEDGGALVGIITRADLLEFVAH
                     RNGEH"
BASE COUNT          346 a          757 c          710 g          372 t
ORIGIN      
        1 gaccccgcta cgcccgccgg aaccgccacc ttcagcgcct ggcgcagatc gtcgatcagg
       61 tcgccagcgt gctcgatgcc caccgagagc cggatcaggt tcggcgccac ctcgaagccc
      121 gagccagcca tgctggcgtg cgtcatctgc cccggatgct cgatcagcga ctcgaccccg
      181 cccaggctct cggccagggt aaagagcctg gtcccggcca caaccgcccg ggcggcgtcg
      241 taaccggccc gcaccgtgaa ggagaccatg ccgccgaaac cgttcggcat ctggcgcgcg
      301 gccacgtcgt ggttggcgtg gcccggcaag ccggggtagt agacggtctc gaccgccggg
      361 tgttccagca gcatggcggc gatcgcttcg ccgtttgagc tatgctggcg catgcgcacg
      421 gccagcgtct tgatgccgcg cagcaccaac caggcgtcga acggtcccgg caccgcaccg
      481 gccgcgttct ggtggaattt cagcgtgtcg tagatctcct cgtcgttgcc taagatcgcg
      541 ccgccgacga cgtcgctgtg gccgcccaga tacttggtcg tgctgtgcaa gaccatgtcg
      601 gcgcccagca ccaggggctg ttgcaggtag ggtgaggcga aggtgttgtc aaccaccagc
      661 cggatcggct tgcccgcttt cacctgggcg atcccggcga tatcgacgat cttgaggtag
      721 ggattggtcg gcgtctcaac ccagatcatg cgcgtgctcg gccggatggc ggcgcgcacg
      781 gcatcgagat tggtcagatc gaccatcgag tagtcgatgc cgtagcgcga ccagaccttg
      841 gaggccagcc ggtaggtgcc gccgtaggcg tcgtcggaca tgatcagatg gtcgccggga
      901 ctgagcagat accaggcgtt ctgcgtcgcg gccaggcccg aggcgtaggt cagcccccac
      961 cgtccgtcct ccagcgccgc cagcgccgtc tgcagcgccg agcgcgtcgg attgtcggtg
     1021 cgcgaatact caaagccctt gtgcttgccg acctcctcct gggcgaaggt cgaggtctgg
     1081 tagatcggca cgatcaccgc gcccgtggtc tcgtccggct cctggccggc gtggatggcc
     1141 agtgtctcaa aatgctcttt ggtcatcagt gctctccgtt tcgatgggcc acgaactcga
     1201 gcaagtccgc tcgggtgatg atgccgacca gcgcaccgcc gtcctcgacc agcacggcgg
     1261 gcgagccggc cgagagatgc ggcacgacgc gctcgacctc ctccttgaca ttgaccagcg
     1321 cgaagggcgg atccatcacc gatgtcacct gctgatggac cgcctccggc gtacggaaga
     1381 cctgatcgag cagcgtccgc tcctgaatgc tgccgacgac atcgtgcacg tcgatgcgcc
     1441 ccgccgacgc gccctggcgc agtaccggca tctgcgagat gcggtagcgg tgcagcagat
     1501 cgatggccgc ttcgaccgtt tcttcgacgc tgacgccgac cacggaaggc agctccggcg
     1561 ccacgcctac tcccgcgcgc tgctgtccga tcaacgtccc cacgcgcgtc gcctgaccga
     1621 agcgggtcaa gaagccgttc tcgcgcatcc agtcgtcgtt gtagaccttc gagagataac
     1681 cacgcccgct gtccggcagg atcacgacca ccagcgtctc gggctggtcc tcccgccgcg
     1741 cgatctccag cgcggcccag acggccgtac cccccgagcc gccgaccagg atgccctcct
     1801 cgcgcgtcac gcggcgcgcg ctcagcagcg agtcgcgatc gccgacctgc acgaactcgt
     1861 ccacgagcga gcggtcgaag gtgccgggcc agaagtcctc gccgatcccc tcgaccttgt
     1921 aggggcgtac gttgtccggg tcggtgtaga tcgaaccggc gggatcggcg ccgatcacct
     1981 gcacggtcgg gttctgctcc ttgaggtact tgccgatgcc gctgatcgtg ccgccggtgc
     2041 ccaccccggc cacgaagtgc gtcacccgtc cggccgtctg ttgccagatc tccggaccgg
     2101 tcgtcagata gtgcgagcgg ggattcatcg ggttgaaata ctggtttggc tggaaggcgt
     2161 tggggatctc gcgcgtcagc cgatc
//
LOCUS       DASUBI010000234         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1053117, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000234 DASUBI010000000
VERSION     DASUBI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053117"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1764)
                     /locus_tag="VFU72_04545"
     CDS             complement(178..1764)
                     /locus_tag="VFU72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422788.1"
                     /translation="MLLAVLLPALGLCGPFPWQTARAAELPYRVEPAMTSSYGSSPRY
                     LSASGSLIVWQDSRGGAPDIYAYDLDDQREFRVARSAAYRTQPAVDGSRIVWVNGSDP
                     EQRKIEGLDLSQGTTLTVTRQPGEVADPAISGHIIVWRQRVDGRWTIEAQDLTDGETW
                     TVSGEADNNAYPAIAGDTVVWQAYRDGHWRIMRYDLTTRTTSTISGGSDDETRPATDG
                     TWLVFLRQPATGGPPRLVLRDLKTGAEKILVQDHLVAQPAVRHGVVAWEDWRTGLPDV
                     YAYDIAQDITFAIARSQQAYQPAVSERGVAWLSQSDMQRGRVQAVAFIPRLPTDPLDP
                     PAVPSADHQYVAETHHFMSSGFKAFWQAHGGPQLLGYPLTEEFSEKDPATGETIIVQY
                     FQRAELEYRASGPEGHKIGLARLGVELTSGRDFPGVQPFGNSADRLYFPETGHSLALG
                     FKEFWDANGGLAMFGYPISEELQENGRTVQYFERARFEFDPSAKDPRYQVVLGLLGQE
                     ALQRKGWIPNPPVDTTMLVP"
     gene            1892..>2212
                     /locus_tag="VFU72_04550"
     CDS             1892..>2212
                     /locus_tag="VFU72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5422789.1"
                     /translation="MAPDGQIPVIMDVDTGVDDAIALLLATRIPELRLLGVTTVAGNV
                     ALDMTTENTLRVLAAAGHAEIPVYRGMAAPLARPHLDAAGFHGANGLADVELPRSPAS
                     VSRVS"
BASE COUNT          340 a          731 c          788 g          353 t
ORIGIN      
        1 gagatgtcac gtcagctcgg atccctcgac cgcgctcggg atgacagggt tagagagggg
       61 cgtaggagta cgacctgccg cactcgtacg cctcatcccc tccccacgtc atcctgagcc
      121 tgtcgaagga tccgtgcccc acgcggcacc ccacgccgtc gagggccacc ggcccgatca
      181 cggcaccagc atggtggtgt cgaccggcgg gttggggatc cagcctttgc gctgcagggc
      241 ctcctggccg agcagcccga gcacgacctg gtagcgcgga tccttggcgc tcgggtcgaa
      301 ctcgaagcgg gcgcgctcga agtactgcac cgtgcgcccg ttctcctgca gctcctcgga
      361 gatcggatag ccgaacatcg ccagcccgcc gttcgcgtcc caaaactcct tgaagccgag
      421 cgccagcgaa tggccagtct ccgggaaata gaggcggtcg gcgctgttgc cgaacggctg
      481 cacccctggg aaatcgcgcc cactcgtcag ctccacgcca agccgcgcca gtccgatctt
      541 gtgcccctcc ggtcccgaag cgcggtactc gagctcggcg cgctggaaat actgcacgat
      601 gatcgtctcg ccggtagccg gatccttctc gctaaactcc tcggtcagcg gatagcccag
      661 cagttgcggg ccgccgtgcg cctgccagaa tgccttgaag ccagatgaca taaagtggtg
      721 cgtctcggcc acgtactggt gatcggccga gggcaccgcc ggcgggtcga gcgggtcggt
      781 cggcagacgc gggatgaagg cgaccgcctg cacgcgcccg cgctgcatat cggactggct
      841 gagccaggcc acgccacgct cggagaccgc gggctggtag gcctgttggg agcgcgcaat
      901 ggcgaaggtg atgtcctggg cgatgtcgta ggcgtagaca tcgggcaagc cggtgcgcca
      961 gtcctcccag gcaaccacgc cgtgccgcac ggcgggctgg gccaccagat gatcctgcac
     1021 caggatcttc tccgcgccgg tcttgaggtc gcgcagcacc agccggggcg ggccgccggt
     1081 cgccggctgg cgcaagaaga cgagccacgt accatccgtg gccgggcgcg tctcgtcgtc
     1141 gctgccgccg ctgatggtgg aagtggtgcg agtggtcagg tcgtagcgca tgatccgcca
     1201 gtggccgtcg cggtaggcct gccagacgac ggtgtcgccc gcgatggcgg ggtaggcgtt
     1261 gttgtccgct tcgcctgaga cggtccaggt ctcaccgtcg gtcaggtctt gggcctcgat
     1321 cgtccagcgg ccgtcgacgc gctggcgcca gacgatgata tggccggaga tggcgggatc
     1381 ggccacttct cccggttggc gtgtcacggt cagcgtggtg ccctggctca gatcgagccc
     1441 ctcgatcttc cgctgctccg ggtcgctgcc gttgacccag acgatacgcg agccgtcgac
     1501 cgcgggctgg gtgcggtagg ccgccgagcg tgccacgcgg aactcgcgct ggtcgtcgag
     1561 gtcgtaggcg tagatgtcgg gcgcgccgcc acggctgtcc tgccagacga taagggagcc
     1621 ggaggcagag aggtatctgg gcgatgagcc gtacgacgag gtcatcgccg gctcgacgcg
     1681 atagggcagt tcggcggcgc gggccgtttg ccagggaaac ggcccgcaca gcccgagcgc
     1741 cggcaggagg acggccagga gcatcagaca ccggctgagc ccgtttgttc gagcgacctt
     1801 catggctgcc gagtatagcc caccgacatg gccgctgccg gctgacgctc tttcgtgcta
     1861 ccgtgcgctc gagtacgagg aggtggttgg catggcacct gacggacaga tccccgtcat
     1921 catggatgtg gacaccggcg tcgatgacgc gatcgcactg cttctggcga cgcgcatccc
     1981 ggagctgagg ctgctcggcg tgaccacggt ggccgggaac gtggcgctgg acatgacgac
     2041 cgagaacacg ctgcgcgtgc tggcggcggc ggggcatgcg gagatcccgg tctaccgcgg
     2101 catggcggcg ccgctggcgc gtccacacct ggacgcggcg gggttccacg gcgccaacgg
     2161 gctggccgat gtcgagctgc cgcgctcgcc ggcgtcggtg tcgcgtgtga gc
//
LOCUS       DASUBI010000235         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_85712, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000235 DASUBI010000000
VERSION     DASUBI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85712"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1401
                     /locus_tag="VFU72_04555"
     CDS             154..1401
                     /locus_tag="VFU72_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5422790.1"
                     /translation="MPPLTPDVPAQADGAGAAFPHGGDEPPHSIADGAPLATAAETPR
                     VPGAASTAVADQLAVPIRSQVAAMANGVASVHETASSHRPAANTPEGAGTPGSVPSGT
                     ALTLDRLTADSLALPPAQDWAVTTPLAIQCFGAFRVRAGETMLAPRRHHKAWELLQLL
                     AAHPPRSLTRDRLIVALWPDPEALLNRNVVNALVGRLREELVAQVPGLAREVVRQARN
                     GDCWLDLDLVTVDVHQWLAIVDREPRLPLAAALAECRQAHALYRPGLLAGASFEWLYP
                     RDEDGLDLAEDYHDTWRRYVLRLARRCVREERADLAIPLYRCLRDAHPRDEAIARELY
                     RCYGRTGDLPGLEREARLLAAALRQEYGDEEDSEALPDLDGPAPETRELFEQIRRQLQ
                     AYSQARTGHPDIGVAAQHHSAGG"
     gene            complement(1623..>2195)
                     /locus_tag="VFU72_04560"
     CDS             complement(1623..>2195)
                     /locus_tag="VFU72_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5422791.1"
                     /translation="ATEQAVAYLYNLGHRQIAHLPGLLHTTVGRDRTNAYCDAMLQRG
                     LAPWVLAAGWTIEEGMAVTETLLSARKLPTAILASSDHTAIGAMHVLRRAGLRVPDDL
                     SVLGFANLPGSELVDPPLTTMGYDREQMGQLMITALFSLVRGEPAPGKIVVPAQFLVR
                     RSCAPPAVAGEERILLRTAPEDGGTSHGHA"
BASE COUNT          381 a          756 c          727 g          331 t
ORIGIN      
        1 agcggtcggc ggcagaatga cacggcgaag gtagtgccgg gccgtgccgg cgcggatacg
       61 ctcctggttc cacccaccgg cgcgcagccc gacgccgctg gtgatgctgg cgaacgtacc
      121 cggccagggg cgctggccgt cgagcacgcg acaatgccgc cactgacgcc tgacgtgccg
      181 gcacaggctg acggagccgg cgccgcattc ccgcatggcg gcgatgagcc gccacacagc
      241 attgccgacg gcgcgccgct cgcgacagcg gccgagacgc cgcgcgtgcc tggtgcggcg
      301 tccacggcgg tagccgacca attggccgtg cccatccgta gccaggtggc cgccatggcg
      361 aatggtgttg cgtccgttca cgaaacggca agcagtcacc ggccagcagc gaacacgccg
      421 gagggggccg ggacgcctgg cagcgttcct tcagggacag cgctcacgct ggaccgtctg
      481 acagccgatt ccctggcgct gcctccagcg caggactggg cagtgaccac accgctggcg
      541 attcagtgct tcggagcgtt ccgggtccgc gccggtgaga ccatgctcgc cccccgccgg
      601 caccacaagg cctgggaact cttgcagctc ctggccgcac acccgcctcg ctcactcacg
      661 cgcgacaggc tcatcgtcgc cctctggccc gatcccgagg cgctgctgaa ccgcaatgtc
      721 gtcaatgccc tcgtcggccg cctgcgtgag gaacttgtcg cgcaggtgcc tggactcgcc
      781 cgtgaagtcg tgcgccaggc acgcaatggc gactgctggc tcgacctcga tctcgtaacg
      841 gtggacgtcc accagtggct ggcgatcgtg gaccgcgagc cgcggctgcc cctggcagcg
      901 gcgctggcgg aatgtcgcca ggcgcacgcg ctctatcgcc ccgggctgct ggcgggcgcg
      961 agcttcgaat ggctctatcc ccgtgacgag gacggcctcg acctggcaga ggactaccac
     1021 gacacgtggc gccggtacgt gctccgcctg gcccgccgct gcgtccgcga agagcgggca
     1081 gacttggcga tcccgctcta ccgctgcctg cgggacgcgc acccacgcga tgaagcgatc
     1141 gcccgcgaac tctaccggtg ttatggccgc accggcgatc taccgggtct ggaacgcgag
     1201 gcacgcctcc tggctgccgc gctccgccag gagtacgggg acgaagagga cagcgaggcg
     1261 ctgcccgacc tcgacggccc agcgccggaa actcgcgagc tcttcgagca gattcgccgg
     1321 caactccaag catattcgca ggcgcgaaca ggtcatccgg acattggcgt cgccgcacag
     1381 caccacagcg ccggtgggta acgcctggga ggcgcaccgg cgatatcgtt ctgactccac
     1441 ccgcctggtc catgatcgtt tcttgaacga cagccatata gaagacgtca cgaaatgccc
     1501 ggctcgctct gcttccagga tggcaaccga cgaggagtga acagaaagga ggagtgaatt
     1561 aatcgggatc gttctcgcgg gcacgaacga ggtgatcggg cggtgcttgt cgccactatt
     1621 gattatgcat ggccatgact agtgccgccg tcttcaggcg cggtgcgcag caggatacgc
     1681 tcctcaccgg ccacggccgg gggggcgcac gatcgccgca ccagaaattg cgcggggacg
     1741 acgattttcc ctggcgcggg ttcaccccga accagactga aaagcgcggt gatcatcaac
     1801 tgccccatct gttcccggtc atagcccatc gtggtcagcg gcggatcgac cagctcggaa
     1861 ccgggcagat tcgcgaagcc cagcaccgat agatcgtccg gcacgcgcag accggcgcgg
     1921 cgcagcacat gcatcgctcc gatcgcggtg tgatcggagg aggccaggat cgcagtgggc
     1981 aactttctgg ccgacagtaa cgtttcagtg acggccatcc cttcttcaat cgtccagccg
     2041 gcggccagca cccacggcgc cagcccacgc tgcagcatcg cgtcacagta cgcgttggtc
     2101 cggtcacgac cgaccgtcgt gtgcagcaag ccgggcagat gggcgatctg gcggtggccc
     2161 aggttgtaaa gataggcgac agcctgctcg gtcgc
//
LOCUS       DASUBI010000236         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1075416, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000236 DASUBI010000000
VERSION     DASUBI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075416"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFU72_04565"
     CDS             <1..331
                     /locus_tag="VFU72_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5422792.1"
                     /translation="IVARVLGLHETALEPDSRFFESGMDSLTAVETRHRLERELDVEL
                     PSTVVFDYPTPAAVADHILSRLEPGVAPLEEDAAEAEALDIDNLPDELVADLLDRQLA
                     QIEELPD"
     gene            328..>2858
                     /locus_tag="VFU72_04570"
     CDS             328..>2858
                     /locus_tag="VFU72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEU5422793.1"
                     /translation="MSANEDQDTARRLKRAAAAIEKLQGRLAALEGSRREAIAVVGLG
                     CRLPGAPDPEAYWRLLRDGVDATTEVPADRWNADALYDAEPATPGRMSTKRFGSIGNL
                     KGFDAAFFGIAPREARSMDPQQRLLLEVAWEALEDAGIPAKSLAGSRTGVYMAACGTD
                     YFQLLLERGFGAIDPYFASGTVHSMAAGRLSYILGLVGPSLSVDTACSSSLVAVHLAC
                     RALRAGDCELALVGGVNLILSPELGVAFSQSRMLSGDGRCKTFSAAADGYGRAEGCGA
                     VVLKRLSRAEADGDRVLAVIRGTAVNQDGATSGLTVPNGPAQQAVIRSALADGGVPAA
                     AVGYVEAHGTGTALGDPIEMGALGAVFGPGRTAAAPLWVGSVKTNIGHAEGAAGIAGL
                     IKVVLALRQGEMPAHLHCAEPSAHIDWRGGSLAVPQTTQPFPERQGRRVAGVSSFGFS
                     GTNAHVVVEAAAEEARSVAADAAPRLYVVSAQSAAALAARAERHAAHLAARAPADWLD
                     LCHTAGAGRAHLAHRLAVVAPSPQAAAARLAAYPSGVRSGRVVGGTAPRVAFLFTGQG
                     AAGAEAGLELAAAGGAFGATLARCGELMKGLVGRPLAEILADGGVWATRADEAQPALF
                     ALQMALVAEWRASGIEPEAVLGHSLGEYAAAVTAGVFPLETGLRLVAARGALMAGLSS
                     EGTMLALPLGEAAARALIGGEAGVSVGAVNGPAATVLSGPRPALARLAARLPAGVEGT
                     WLAVTHAFHSPQMAPLVEAYREVVEAEHARTPFQMPRLGFLSTLMGRVVREEVCDPGY
                     WCRQLVEPVMFWPAVTGLAASGATHAVEIGPRPVLGGLARGCVGP"
BASE COUNT          334 a          842 c         1260 g          422 t
ORIGIN      
        1 catcgtggcg cgcgtgcttg gcctccacga gacagcactc gagcctgaca gtcgcttttt
       61 cgaatcgggc atggacagcc tgacggctgt cgagacacgc caccggctcg agcgggagct
      121 cgatgtcgag ctgccatcga cggtcgtctt cgactatccg acgcccgcgg ccgtcgccga
      181 ccatatcctg agccggctcg agcccggcgt ggcccccctc gaggaagacg ctgccgaagc
      241 ggaagctctc gacatcgaca atctgccgga tgagctcgtc gcggatctgc tggatcgcca
      301 gcttgcccaa atcgaggagc tgccggattg agcgccaacg aagaccagga cacagcgcga
      361 cgccttaagc gtgccgccgc cgccatcgag aagctgcagg gtcggcttgc ggcgctggag
      421 ggctcgcggc gcgaggcgat cgccgtggtg gggctcggct gccgcctgcc cggggcgccg
      481 gatccggagg cttactggcg tctgctgcgc gacggcgtcg atgcgacgac ggaggttccg
      541 gccgatcgat ggaacgccga tgcgctctat gatgccgagc cggcgacgcc tgggcgaatg
      601 agcacgaagc gcttcgggtc catcggcaat ctgaaagggt tcgacgcggc gtttttcggg
      661 atagcgccgc gtgaggcgcg cagcatggat ccgcagcagc ggctgttgct ggaggtcgcc
      721 tgggaggcgc tcgaggatgc gggcattcca gccaagagcc tcgcgggcag ccgcaccggc
      781 gtctacatgg ccgcctgcgg caccgattac ttccagctcc ttctcgagcg cggcttcggc
      841 gccatcgatc catacttcgc ctccggcacg gtccacagca tggcggctgg tcggctctct
      901 tatattctcg gtctcgtcgg tccgagcctg tcggtggaca cggcgtgctc gtcgtcgctg
      961 gtggcggtgc acctggcgtg ccgggcgctg cgcgcgggcg actgcgagct ggccctggtg
     1021 ggcggcgtca acctcatcct gtcgccggag ctcggtgtcg cgttttcgca atcgcggatg
     1081 ctgtctgggg acggtcggtg caagacgttc tcggcggcgg cggacgggta cggtcgggcg
     1141 gaaggctgcg gggccgtggt gctgaagcgg ctgagccggg cggaggccga tggcgaccgg
     1201 gtgctggcgg tgatccgggg cacggcggtg aaccaggacg gggcgacgag cggtctgacg
     1261 gtgccgaacg ggccggcgca gcaggcggtg atccggtcgg cgctggcgga cggcggggtg
     1321 ccggcggcgg cggtgggcta cgtcgaggcg cacgggacgg ggacggcgct gggcgacccg
     1381 atcgagatgg gggcgctggg ggcggtgttc gggccgggcc ggacggccgc ggcgccgctg
     1441 tgggtggggt cggtgaagac caacatcggg catgccgagg gggcggcggg gatcgccggg
     1501 ctgatcaagg tggtgttggc gctgcggcag ggcgagatgc cggcgcacct gcattgtgcc
     1561 gagcccagtg cgcacatcga ctggcggggc ggttcgctgg cggtgccgca gacgacgcag
     1621 ccgttcccgg agcggcaggg ccggcgggtg gcgggggtga gctcgttcgg cttcagcggc
     1681 accaacgcgc atgtcgtggt ggaggcagcg gccgaggaag cgagatcggt cgcggccgat
     1741 gccgcgccgc ggctttatgt tgtgtcggcg caaagcgcgg cggcgctggc ggcgcgggcg
     1801 gagcggcatg cggcgcatct ggcggcgcgt gcgccggcgg actggctgga cctgtgccac
     1861 acggcggggg cgggccgggc gcacctggcg caccggctgg cggtggtggc gccaagcccg
     1921 caggcggcgg cggcgcgcct ggcggcttac ccgtcggggg tgcggtcggg ccgggtggtg
     1981 gggggaacgg cgccgcgggt ggcgttcctg ttcacgggcc agggcgcggc cggggcggag
     2041 gcggggctcg agctggcggc cgcgggcggg gcgttcggcg cgacgctggc gcgctgcggc
     2101 gagctgatga aggggctggt cgggcggccg ctggcggaga tcctggcgga tggcggcgtg
     2161 tgggcgacgc gggccgacga ggcgcagccg gcgctgtttg cgctgcagat ggcgctggtg
     2221 gcggaatggc gggcgagcgg gatcgagccg gaggcggtgc tggggcacag cctcggggag
     2281 tatgcggcgg cggtgacggc gggggtgttc ccgctggaga cggggctgcg gctggtggcg
     2341 gcgcggggcg cgctgatggc ggggctttcg tcggagggca cgatgctggc gctgccgctg
     2401 ggggaggcgg cggcgcgggc gctgatcggg ggcgaggcgg gggtgagcgt gggggcggtg
     2461 aacgggcctg cggcgacggt gctgtcgggt ccgcggccgg ctctggcgcg cctcgcggcg
     2521 cggctgccgg cgggggtgga ggggacatgg cttgcggtga cgcacgcgtt ccattcgccg
     2581 cagatggcgc ccttggtgga ggcctatcgc gaggtggtgg aagcggagca cgcgcggacg
     2641 ccgttccaga tgccgcggct cggctttttg agcacgctga tggggcgagt ggtgcgggag
     2701 gaggtgtgcg accccgggta ctggtgccgg cagctggtgg agccggtgat gttctggccg
     2761 gcggtgacgg ggctggcggc gtcgggggcg acgcatgcgg tggagatcgg gccgcggccg
     2821 gtgctgggtg ggctggcgcg ggggtgcgtg gggccgga
//
LOCUS       DASUBI010000237         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_562372, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000237 DASUBI010000000
VERSION     DASUBI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562372"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..>2216)
                     /locus_tag="VFU72_04575"
     CDS             complement(283..>2216)
                     /locus_tag="VFU72_04575"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422794.1"
                     /translation="TLAGQRAPLDALADALRSRSALLVLDNCEHLPQVAPVVRALLTA
                     APRLRLLATSRAPLELEGETLLRLEGLPVPPVDAPASDPGSFAGVRLFLDRATRLTPG
                     WGQDQEELAGAVRLCRLLDGLPLGIELAAHWVGHFSPDEIAAELHDNLDFLAARTRDV
                     PERQRSLRAVFDYTWRLLSPAEQQALARLSVVRGGFDREAARAVGSVRATTLVMLADK
                     ALLRHAGSGRYGLHELLRQFAAEQLEARGELEATRDRHRDWCLTLNGSPHSALPNPEQ
                     AARLERDQDNVRAALRWTIERRDVEGALRLGISDGAYWYLRGQFAEGRAWLGQVLRLN
                     APPQLAALRSRALAYAGLLAYCEGDLRSAEPLLRDAATLAKDAVDSETVGLCELYLGH
                     LARARGSRTQAEQRYEHALQMARRSDGECWDTIVLSALTRLRYDNGDFAGASAAADET
                     LALCAVRDDPSTRARTLAIRSRLAALAHEDALADALMAECLQLQQRLRAPLEHAYAHL
                     MAAETALDRRRLDEAARHLDEGLRLAAQVHYQLALARGLEGVAELLASAEPEAALQLG
                     GTATSIRAAHGLTPNPIEAARLERWLDVARAAVGAEEAERMQQVGRARSAADGLAQAR
                     AACAAIAGAPITAQPVSSP"
BASE COUNT          329 a          784 c          787 g          316 t
ORIGIN      
        1 ggcgcggagt acttcgagca tcgtctccgc ccactgcgtc aatgcgggac tcccttcgtg
       61 agcgatcagc gcggggtggt gcgcggtgat gcgctctccc tccccggcga gcaggcgagt
      121 ggcacctggt cgcgtggcta actctaggga gcgaagatga gcgtcaggta agaattcagt
      181 gagaagattc taatggaggt cgcgttgacg gggtgagaag cgcaggaaag ccccggtacg
      241 aggcccacgg ctgggtatgg gagcgcgtcg acggcagggt ggttagggtg aggacaccgg
      301 ctgggcagtg attggcgcgc ccgcgatcgc cgcgcaggcc gcgcgcgcct gggccagccc
      361 gtctgccgct gagcgcgccc ggcccacctg ttgcatgcgc tcggcctcct ccgcgccgac
      421 ggctgcgcga gcgacgtcta gccagcgctc cagccgcgct gcttcgattg ggttgggcgt
      481 caggccgtgc gccgcgcgga tgctcgtcgc ggtcccacca agctggaggg ccgcttccgg
      541 ctcggccgaa gccagcagct ccgccacgcc ctccagcccg cgggcgagtg ccaactgata
      601 gtgtacctgg gcggccagcc gcagcccttc atcgagatgc cgcgccgctt catcgagtcg
      661 gcgtcgatcg agcgccgtct cggcggccat gagatgggca taggcgtgct ccaggggagc
      721 gcgcaatcgt tgctgcagct gcagacattc ggccatgagc gcgtccgcca gtgcgtcctc
      781 gtgggccagc gcagctagcc ggctgcgaat ggccagcgtc cgggcgcggg tggacggatc
      841 atcacgcacc gcgcacagcg ctagcgtctc gtccgcggcc gcgcttgcgc cggcgaagtc
      901 gccattgtcg tagcggagcc tggtcagcgc gctcagcacg atcgtgtccc agcactcgcc
      961 atccgaccgg cgcgccatct gaagagcgtg ctcgtaccgc tgctcggcct gagttctgga
     1021 cccgcgcgcc cgcgccaggt gcccgaggta gagttcgcat aggccaactg tttcgctgtc
     1081 aacggcgtcc tttgccagcg tggccgcatc gcgtagaagc ggctcggccg accggagatc
     1141 gccctcacag taggccaaca ggccggcata ggcgagcgct cggctgcgga gcgcggcgag
     1201 ctgcggcggt gcgttgagcc tgagaacctg tcccagccag gcacgccctt ccgcgaactg
     1261 cccgcgcaga taccagtacg cgccgtcgct gatccccagc cgcagcgcgc cctcaacatc
     1321 ccggcgctcg atggtccagc gcagcgccgc acgcacgttg tcctggtcgc gttcgagccg
     1381 cgccgcctgt tcgggattgg gcagcgccga atgcggagat ccgttgaggg tcaagcacca
     1441 atcgcgatgc cggtcgcgcg ttgcctccag ctcgccgcgc gcttcgagct gctcggcggc
     1501 gaactggcgc agcagctcgt gcaggccgta gcggccggac cccgcatgcc gcagcagcgc
     1561 tttgtccgcc agcatgacca gggtcgtcgc ccgcacgctg ccgacagccc gcgccgcttc
     1621 gcggtcgaag ccaccccgga cgacggagag ccgcgccagc gcctgctgct cggccgggct
     1681 gagcaggcgc caggtgtagt cgaagacggc ccgcaggctg cgctggcgct ccggcacatc
     1741 gcgcgtgcgc gcagctaaaa aatccaggtt gtcatggagc tcggcggcga tctcgtccgg
     1801 gctgaaatgg ccgacccagt gcgccgccag ctcgatcccc agcggcagcc cgtcgagcag
     1861 ccggcagagc cgcaccgcgc ccgcgagctc ttcctgatcc tggccccagc caggcgtcag
     1921 gcgcgtcgca cgatcgagga agaggcgcac tccggcgaaa ctgccggggt cggaggcggg
     1981 ggcgtccacc ggcggcaccg gcagcccctc cagtcgcagc agcgtttcac cctccagttc
     2041 gagcggggcg cgcgaggtgg cgagcaggcg gagtctcgga gcagccgtca gcagggcacg
     2101 gaccaccggg gccacctgcg gcaagtgctc gcagttgtcg agcaccagca aggcgctgcg
     2161 cgaccgcagc gcgtccgcca gcgcgtcgag cggcgcgcgc tggccggcca gcgtca
//
LOCUS       DASUBI010000238         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_548667, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000238 DASUBI010000000
VERSION     DASUBI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548667"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VFU72_04580"
     CDS             complement(<1..1184)
                     /locus_tag="VFU72_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5422795.1"
                     /translation="MTTGLVLPLAWLFGVLLRDAGAGWLVVAGLAVLAGLAALLSRRR
                     VQRVVILLLLCLSLAGFWRAQVSPPAPATLPSRSPMTLRAEVAGRPLPVSGRVHARLR
                     PLDSPSAIDANLPALPGVHQGDIVLVRGGLSPGQPATLFVTSFEVEGSLASRPLRLRN
                     RLGDSLRAAILGHIPEPAGSLTLGVVTGDESGMTGATRQAFRQAGLSHITAVSGWNLA
                     LVAAALALLIRGSRLPRLTRLGLLLGGIWLYAFLVGLDASVVRAAAMSSLFLLARWRG
                     RPADIIAALLWGITAVLAVWPALRFDTGFQLSVAATVALALIAPKLVGRPPWQVALAV
                     PAAAELAVAPLTLHHFGSFALLSPLSNFLVEALIGPVMAGGVLVALASWWLPLLADLL
                     GAV"
     gene            complement(1181..1747)
                     /locus_tag="VFU72_04585"
     CDS             complement(1181..1747)
                     /locus_tag="VFU72_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU5422796.1"
                     /translation="MEMSRLRLLLLGITVALATAIITLVVVGRRTPDLVLSVEPVAAD
                     AQITVYVGGAVARPGLYTLPARSRVADALTQAQALDASDLARLPMAAVLHDGQQVIVP
                     ERAAGTPPATAAASRATPTPQGPIDVNSASAAELEALPGVGPVLAQRIVAYRDEHGPF
                     ATLDALDAVQGVSARMVDGWRQMATVGP"
     gene            1937..>2865
                     /locus_tag="VFU72_04590"
     CDS             1937..>2865
                     /locus_tag="VFU72_04590"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5422797.1"
                     /translation="MSLKSLLPLLKTDASLAALRGRVDQLAPGQAVSVPALPSAARVA
                     TLATLLTGNQRPALVLTGRLDAAEELAAALAEYLPAERAPLIWPVPEALPYEQVPVDR
                     VASARRVDILIQLLDHQPVVVVAPARALAQPIAPPSELARQRLTIRVGQRLRQDVLIR
                     DLLDQGYELVSLVTEPGQVSRRGGIVDLFLPSGDDAVRIDLFGDEVDALRLFDPETQR
                     SIKSLDAFEALPAFEVTLDRRRKAAEHLRALEDGSLRPEVAEEWARTIRRLEHGDPRT
                     GLDLITPYLLPRPASLLDYLPDDHLLAVVEPTS"
BASE COUNT          458 a         1024 c         1033 g          350 t
ORIGIN      
        1 acggcgccca ggagatccgc cagcagcggg agccaccaac tcgccagcgc caccaagacg
       61 ccaccggcca tcaccggacc gatgagcgcc tcgacgagga agttgctcag gggcgagagc
      121 agggcgaagc tgccgaagtg atgcagcgtc agcggcgcca ccgccagctc ggcggccgcc
      181 ggcacggcca gcgccacctg ccagggcggc cgccccacca gcttcggtgc gatcagggcc
      241 agcgcgacgg tggcggccac cgagagctga aagccggtgt cgaagcgcag cgccggccag
      301 acggccagca cggcggtaat gccccacagc aatgcggcga tgatgtcggc cgggcggccg
      361 cgccagcgcg ccagcagaaa gaggctgctc atggcggcgg cacgcaccac cgaggcatcg
      421 aggccgacca ggaaggcgta gagccagatg ccgcccagga gtagaccgag gcgcgtcagc
      481 ctgggcaggc ggctgccgcg gataaggagt gccagcgcgg cggccactag cgccaggttc
      541 cagccgctga cggcggtgat gtgcgacagg cccgcctggc gaaacgcctg ccgggtggcc
      601 cccgtcatgc cactctcgtc gccggtgaca acgccgagcg tgagggaccc cgccggctcg
      661 gggatatgcc cgaggatggc ggcgcgcagg gagtcaccga gccggttgcg caagcgcagc
      721 ggacggctgg ccagcgaacc ctcgacctcg aagctggtca cgaagagcgt cgccggctgg
      781 cccggcgaca ggccaccgcg caccagcacg atgtcgccct gatgcacgcc gggcagcgcg
      841 gggaggttgg cgtcgatcgc ggaaggagag tcgagcggcc ggagccgagc atgtactcgc
      901 cccgagaccg gcagcggacg cccggctacc tcggcccgca gcgtcatcgg actgcggctg
      961 gggagcgtgg ccggggcggg cggggagacc tgcgcgcgcc agaaaccggc gagggacagg
     1021 cagaggagca gcaggattac gacgcgttgc acccgtctcc gcgataacaa ggccgccagt
     1081 cccgcgagca cggccagacc ggcaacgacc agccacccgg cgccggcatc gcgcagcagc
     1141 acgccgaaca accaggcgag cggcaggacg aggccggtcg tcacggccct acggtcgcca
     1201 tctggcgcca gccatcgacc atgcgcgccg agacgccctg caccgcgtcg agcgcgtcga
     1261 gcgtggcgaa tggcccgtgc tcgtcgcggt aggccacgat gcgctgggca agcaccggcc
     1321 caacccccgg cagcgcctcc agctccgccg cactggcgga gttgacgtcg atcggcccct
     1381 gcggcgtcgg cgtcgcccgg ctcgcggcag cggtggccgg gggagtgccg gcggcgcgct
     1441 cgggaacgat cacctgctgc ccgtcgtgca gcaccgccgc catcggcagc cgggccaggt
     1501 cggaggcgtc gagcgcctgc gcctgggtca gcgcatcggc cacccgagag cgggcgggca
     1561 gcgtgtagag gccggggcgc gccaccgcgc cgccgacgta cacggtgatc tgggcatcgg
     1621 cggctaccgg ctcgaccgac agcaccaggt cgggcgtgcg ccggcccacg accaccagcg
     1681 tgatgatcgc cgtggccagc gcgaccgtga ttccgagtag gagtaagcgc aagcgcgaca
     1741 tctccatggc gcgagtccct ccctgccgag cgccagttgg aatgcgtaca cgcgagtggc
     1801 cgcaatgtac tcgctcttgt ctcgattgca tagggggcct gcgcacaagt gggcggttgg
     1861 tatactagta agcgaaactc gacagggccg gtcccgccga ccggccctct ttggcgtgtc
     1921 ggaaggcggc tctccggtgt ctctcaaaag cctgctcccc ctcctcaaga ccgacgccag
     1981 cctggccgcg ctgcgtggtc gggtcgatca gcttgccccc ggtcaggcgg tcagcgtgcc
     2041 cgcgctgccg agcgccgcgc gcgtggcgac gctggccacg ctgctgacgg gaaaccagcg
     2101 tccggcgttg gttctgaccg gacggctcga cgccgctgag gagctggccg cggcgctggc
     2161 cgagtacctg ccggcggagc gcgccccgct gatctggccc gtgcccgagg cactgcccta
     2221 cgaacaagtg ccggtcgatc gcgtcgccag cgcccggcgc gtcgacatcc tgatccaact
     2281 gctcgatcat cagccggtgg tcgtggtcgc gccggccagg gcgctggcac agccgatcgc
     2341 gccgccgtcc gaattggcgc gccagcggct gacgatccgc gtcgggcagc ggctgcgcca
     2401 ggatgtgctg atccgcgact tgctcgacca gggctacgag ctagtgtcgc tggtcaccga
     2461 gccggggcaa gtcagccgtc ggggcgggat cgtcgacctc ttcctgccca gcggcgacga
     2521 cgccgtgcgc atcgatctct tcggcgatga ggtcgatgcc ctacgcctct tcgatcccga
     2581 gacgcagcgc tcgatcaagt cactggatgc attcgaggcg ctgccggcat tcgaggtcac
     2641 gctcgaccgc cggcgcaagg ccgctgagca cctgcgcgcg ctcgaagacg gcagcctgcg
     2701 gccggaggtg gccgaggagt gggcgcgcac catccggcga ctggagcacg gcgacccgcg
     2761 caccgggctc gatctgatca cgccctatct actgccgcgg ccggcctcgc tgctcgacta
     2821 ccttcccgac gatcacttgc tggccgtggt tgagccgacc tcgat
//
LOCUS       DASUBI010000239         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_702159, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000239 DASUBI010000000
VERSION     DASUBI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702159"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFU72_04595"
     CDS             complement(<1..804)
                     /locus_tag="VFU72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5422798.1"
                     /translation="MRLIIRAERVFDGSGAPPQAAEVVIEGERIVEVNHGAGTLARDD
                     DQVLELREGTLLPGLIDAHVHLIGSGEPGDRAFGLGDVVNSIPTVTLNCFRNAQRDLE
                     AGFTTVRDAACRYYADIALRDAINRGELTGPRIWACGLGITSTAGHMDREKILPPHLS
                     LPGPSAVADGPVEARTAVRQNLRYDVDFIKFNATLTEHVRRYQAYCAPEMTAETMRAL
                     IEEAHWHGRRVTAHCYGGPGADWALDAGIDGIEHGFYLTDEQLGRMAEQG"
     gene            complement(829..1590)
                     /locus_tag="VFU72_04600"
     CDS             complement(829..1590)
                     /locus_tag="VFU72_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5422799.1"
                     /translation="MPDEVLIERMSWPQVRDALAAGKRTVLLPVAAMEQHGPHMAIGT
                     DTFLGYETARRLAEALGDALVAPAISIGYSVGHLPMPGTVTISEETLTTVITEVADSL
                     AHHGFRDIVFVISHGGNYGAVNAALPRLRAAHPEVRFHAQTDFESGMGDRAALYRELG
                     VEARRMGVHAGQGETSMMLASHPELVRMEAAVEGFTGDASIRWKAKVPPPMDEMSPTG
                     ILGDARGSTAEIGEALFNQNITRWVDAIRAGTYAG"
     gene            complement(1603..>2145)
                     /locus_tag="VFU72_04605"
     CDS             complement(1603..>2145)
                     /locus_tag="VFU72_04605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5422800.1"
                     /translation="NGSFYIAGGGRGDIDLTFDSMRYARQFLPNYVKNRSIFRLRVGR
                     RLLGDVAQSLRGAAAGRRPSLAREGDVEPKPDLDLARRSRDRFTELFPALGPVRLHRV
                     WAGYIDSLPDAVPVLGETGQVQGFILATGFSGHGFAMGPIVGQLMAELIVDGQPSVDI
                     RTLSYERFLTGHIARPKSVV"
BASE COUNT          311 a          734 c          769 g          331 t
ORIGIN      
        1 gccctgctcg gccatgcgcc cgagctgctc gtcggtgagg tagaaaccgt gctcgatgcc
       61 gtcgatgccg gcgtccagcg cccagtcggc ccccggcccg ccgtagcagt gggcggtcac
      121 ccgccgcccg tgccagtgcg cctcctcgat cagcgcccgc atcgtctcgg ccgtcatctc
      181 gggggcgcag taggcctggt agcggcgcac gtgctcggtc agagtggcgt tgaacttgat
      241 gaaatccacg tcgtagcgca ggttctggcg cacggcggtg cgcgcctcga ccgggccgtc
      301 ggccaccgcg ctcgggccgg gcagcgagag gtgcggcggc aggatcttct cgcggtccat
      361 gtggccggcg gtcgaggtga tgccgaggcc gcaggcccag atgcgcggac cggtcagttc
      421 gccccggttg atcgcgtcgc gcagcgcgat gtcggcgtag tagcggcagg cggcgtcgcg
      481 cacggtggtg aagccggcct ccaggtcgcg ctgggcgttg cggaagcagt tgagcgtgac
      541 ggtagggatc gagttgacga catcgcccag cccgaaggcg cggtcgcccg gctcgccgct
      601 gccgatcagg tgtacgtgag cgtcgatcag ccccggcagc agggtgccct cacgcagctc
      661 gagcacctgg tcgtcgtcgc gcgccagcgt gccggcgccg tgattgacct cgacgatgcg
      721 ctcgccctcg atcaccacct cggcggcctg tggcggcgcc ccgctgccat caaacacccg
      781 ctccgctcgg atgatcagtc gcatgtacgc catcctctcg tgcgtgcgtc agcccgcata
      841 ggtgccggcg cggatcgcat cgacccagcg cgtgatgttc tggttgaaga gcgcctcgcc
      901 gatctcggcg gttgagccgc gggcgtcgcc gagaatgccg gtcgggctca tctcatccat
      961 cggcggcggc accttcgctt tccagcgaat cgaggcgtcg ccggtgaaac cttcgacagc
     1021 ggcctccata cgcaccaact cggggtggct ggcgagcatc atcgaggttt cgccctggcc
     1081 ggcgtggacg cccatgcgcc gcgcctcgac gcccagctcg cgatagaggg ccgcgcgatc
     1141 gcccatgccg ctctcgaagt cggtttgggc gtggaagcgg acctcggggt gggcggcgcg
     1201 cagccggggc aaggcggcgt tgaccgcccc gtagttgccg ccgtggctga tcacgaagac
     1261 gatgtcgcga aagccgtggt gggccaggga gtccgcgacc tcggtgatga cggtggtgag
     1321 tgtctcctcg gagatggtca ccgtgccggg catcggcaga tgaccaaccg agtagccgat
     1381 gctgatggcc ggcgcgacca gcgcgtcgcc gagcgcctcg gccagccgcc gcgcggtctc
     1441 gtagcccaga aaggtgtcgg tgccgatcgc catgtgcggt ccgtgctgct ccatcgcggc
     1501 gaccggcagc aggacggtgc gcttgccggc ggccagcgcg tcacgcacct ggggccagct
     1561 catccgctcg atgagaacct catccggcat ggttcgctcc aatcagacga cgctcttcgg
     1621 ccgggcgatg tggccggtga ggaagcgctc gtaggacaac gtgcggatat cgaccgaggg
     1681 ctgcccatcg acgatcagct ccgccatcag ttgcccgacg atggggccca tggcgaagcc
     1741 gtggccgctg aagccggtcg ccaggatgaa cccctgcacc tgcccggttt caccgagcac
     1801 cggcacggca tcggggagcg agtcgatgta gccggcccag acgcggtgca gccgcaccgg
     1861 cccgagcgcg gggaagagct ccgtgaagcg atcgcggctg cgccgcgcga gatcgaggtc
     1921 gggcttgggc tcgacatcgc cctctcgtgc cagggacggc cgtcgcccag cggccgcccc
     1981 gcgcaggctc tgggcgacgt cgccgagcag gcgacgccca acgcgcaacc ggaagatact
     2041 ccgattcttg acgtagttcg gcaggaattg ccgggcgtag cgcatcgagt cgaacgtcag
     2101 gtcgatgtcg ccgcgcccgc cgcccgcgat gtagaagctg ccgtt
//
LOCUS       DASUBI010000240         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_85633, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000240 DASUBI010000000
VERSION     DASUBI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85633"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFU72_04610"
     CDS             complement(<1..643)
                     /locus_tag="VFU72_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5422801.1"
                     /translation="MAALSHLTPAAPRPLLVGREREQAVLRDCLAHALTGQGSLVLLS
                     GEAGIGKTALAEVLLAEAATQGALVLVGRCYDLSETPPYGPWAEALARAPHDADLPVL
                     PTAVLPPDQAGETLASQEAIIARVQAYLAALAARQPLVLLLDDLHWADPASLDLLRVL
                     ARDLADLPLLLLVTYRSDELTRRHLLYQLLPLLVRESHAQRLDLHPLDDTGLRA"
     gene            complement(846..2033)
                     /locus_tag="VFU72_04615"
     CDS             complement(846..2033)
                     /locus_tag="VFU72_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422802.1"
                     /translation="MNIPRRRGHRHALSALLIVLALAGCAPAGTTTALARPGTATAPP
                     PAAAVTPSTTPTALAQAPASASPSGATSRCPEQATPPSAVPTVASAASPAAGLPAAGR
                     VIAAIPVGRGPVDYPAAGAGAIWIPNRGDGTISRLDPQTNQVAATICVGGNGGAADGS
                     DFAVAVAGQSVWATRYTGGERSVVRLAPATNQIAATFPVDVLPFALAAGDDSLWLTSF
                     GDDALERLDPQSGRVVARLAVDKPTAVAVGAGAVWVVLHRHSQVARIDPATNQIVATI
                     AVGDQPEQLTVGEGGVWVSSPGDRSVWRLDPQTNQVAATIPVAPGPFGVAAGGGAVWV
                     VSLGGAANRYVATLSRIDPATNQVAGVSALRPGARSVTLAAGAAWVTSSAGDLDLVER
                     IAP"
     gene            complement(2274..>4338)
                     /locus_tag="VFU72_04620"
     CDS             complement(2274..>4338)
                     /locus_tag="VFU72_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5422803.1"
                     /translation="QVLDARVDRLGAAARDLLTIAAVIGQDAPLALWQAVAGAPEDDL
                     LAVVERAAAARLLDPTADGLRFAHALVREALYERVLPPRRRRLHRQVAEALLTTPAPD
                     PDAVAYHFQQAGDPQAVAWLIAAGERAQMAYAWLTAADRYEAALALDTGVLAPRERAV
                     ALLTLAQLRRYSDPQRGSISAAEAVCLAEDAGDALLATAARFDQAHLRCLTDDLAQGL
                     PQLEAAFASLASVPLAVRAQLPAPRIQGIRPDEDYHRGALVNWLNHAGRYAEALAAGA
                     PIAARAAGTTARGLAGLAQTYAALGQPDEARRAITDARAAFAAAGQYQETGQMWLGEF
                     QWIVWPYQTDQRVAYQHLLVAARQAFARAAETMGTRRPWEGTMLLDILWLEGHWEDAR
                     AQACAEPETFRAQRLLALLSRAQGDPAPAWQYVRDEPYASAIPGGARFQRVTDRQRLA
                     AALALDAGDLPTARAWLETYDRWLAWSGAVLGRAEGCLGWAAYYRAVGDLGRARHHAD
                     QALAHASAPRQPLALLAAHRLLGELVTAAGQQTEAATHLAAALALADACAAPYEWALC
                     LLAQAELQAASGDRQQALAAIAEARTLLEPLQARLALARADALTARLAFLKPSAPVRP
                     ANLTPREMEVLRLLAAGRSNREMAAALSLSARTVERHITNLYRKLDVRGRAQATAFAL
                     HHHLT"
BASE COUNT          601 a         1552 c         1621 g          564 t
ORIGIN      
        1 gcgcccgtag tccggtatcg tccagcgggt gcaggtccag ccgctgtgcg tgggactcgc
       61 gcaccagcag gggcaacagt tggtagagca ggtgccggcg ggtgagttcg tcgctgcggt
      121 aggtgacgag cagcagcagg gggaggtcgg cgaggtcgcg cgccagcacg cgcagcaggt
      181 cgagcgaggc tgggtcggcc cagtgcaggt catccagcag cagcacgagc ggctgccgcg
      241 ccgccagcgc cgcgagatac gcctgcacgc gcgcgatgat ggcctcctgg ctcgccagcg
      301 tctccccggc ctggtcgggt ggcagcactg ccgtgggcag gaccggcagg tcggcatcgt
      361 gtggggcgcg ggcgagcgcc tcggcccacg gcccataggg gggcgtctcg gagaggtcgt
      421 agcagcgccc gaccaggacc agcgccccct gtgtggccgc ctcagccagc aagacttcgg
      481 ccagcgcggt cttgccgatg ccggcctcgc cgctgaggag caccaggctg ccctggccgg
      541 tcagggcgtg ggccaggcag tcgcgcagga cggcctgctc gcgctcccgg ccgacgagga
      601 gcgggcgggg ggccgccgga gtgagatggg acaatgcggc catggcgcac ctggacgatg
      661 ccattgcggt tgcttctacg tcagcatagt gcatgagtat agcagcgaga ggacacagcg
      721 tcaccgccga gatcgtcatt ggacggccag gcagattcgg gcgtggtccc aaccccagcc
      781 accaaattcc ggcgccactg cggttgtcca ggtccgtggc gcctctccat gccgtcggac
      841 cacgactacg gtgcgatgcg ctccaccaga tcaaggtcgc ccgcactgct ggtgacccag
      901 gccgctcccg ccgccagggt gacgcttcgc gcgccggggc gcagcgcgct tacgccggcg
      961 acctgattgg tcgcagggtc aatccggctc agcgtcgcca cgtaccggtt cgccgcgccc
     1021 ccgaggctga cgacccagac cgccccgcca ccagcggcga cgccgaaggg gccaggggcg
     1081 accgggatgg tcgccgcgac ctgattggtc tgcgggtcga gccgccagac cgaccggtcg
     1141 cccggactgc tcacccacac gccgccctcg ccaacggtga gttgctcggg ctgatcgccc
     1201 accgcgatgg tcgcgacgat ctggttggtg gcggggtcga tccgcgcgac ctggctgtgg
     1261 cggtggagca ccacccagac ggcgccggcg ccgacagcca ccgcggtggg cttgtcaacc
     1321 gcgagccggg ccaccacgcg gccactctgc gggtcgaggc gctccagcgc gtcgtccccg
     1381 aaggaagtga gccagaggct atcgtcgccg gcggccagcg cgaaaggcag gacatcaacc
     1441 gggaacgtcg cggcgatctg attagtcgcc ggggcaagac gcaccaccga ccgctctccg
     1501 ccggtgtacc tggtcgccca gacgctctgc ccggcgaccg cgaccgcgaa atccgaacca
     1561 tccgccgccc caccgttgcc gccgacgcag atggtcgcgg ccacctgatt ggtttgggga
     1621 tcgaggcgcg agatggtgcc atcgccccgg ttggggatcc agatggctcc agcgccggcg
     1681 gccgggtagt caactggccc ccggcccacc ggaatcgccg cgatgacccg gccagcggcc
     1741 ggcagccccg ccgccgggct cgccgcactg gccacggtcg gcacggcgct cggcggcgtc
     1801 gcctgctctg ggcagcgact ggtcgcgccg ctgggtgagg cactggccgg cgcctgggcc
     1861 agcgcggtgg gggtggtgga cggcgtgacc gcggcggcag gcggtggtgc cgtggcggta
     1921 ccgggccggg ccagggcggt ggtggtgccg gccggcgcgc acccggccag cgcgagcacg
     1981 atgagcaggg cagacagagc gtggcggtgt ccccggcgtc gtggtatgtt catggtgccc
     2041 tcccgggtgt gctggacgac agcaacgaac catgccgtcg tcgcgcgaac gccgcgacgg
     2101 tgtcgaggat ccgccgggtg tcccaggtca tgcccgtgac gtccggcgaa gtcctcgaca
     2161 tgaagcactg tagcgccaac gccaggacac gacttccggt aaaccacgta attcgctgcg
     2221 ccgcgacgag tccggcgtcg tcgctagggc tgccggaaac cccgtagtcg tcgttatgtc
     2281 agatgatggt gcagggcaaa ggcggtggcc tgcgcccggc cacgcacgtc gagtttccgg
     2341 tagaggttgg tgatgtggcg ctcgaccgtc cgggcgctga gactgagagc cgccgccatc
     2401 tcccggttac tccgcccggc ggcgagcagc cgcagcacct ccatctcgcg cggcgtcagg
     2461 ttggcggggc gcaccggggc agacggtttc aggaacgcca accgcgcggt cagcgcgtcg
     2521 gcgcgggcca gcgcgagccg cgcttggagc ggctcaagga gcgtgcgggc ctcggctatc
     2581 gccgccagcg cctgctgccg gtcgccgctg gcagcctgca actctgcctg ggcgagcagg
     2641 cacagcgccc actcataggg cgcggcgcag gcatcggcca gcgcgagggc ggcagcgaga
     2701 tgcgttgcgg cctcagtctg ctggccggca gcggtgacga gttcgccgag cagccggtgc
     2761 gcggcgagga gggcgagcgg ctggcgcggc gcgctggcgt gggcgagcgc ctggtcggca
     2821 tggtgccgcg cccggccgag gtcgcccacc gcgcggtagt aggcggccca ccccaggcag
     2881 ccctcggccc gccccagcac cgcgccagac caggcgagcc agcggtcgta cgtctccagc
     2941 caggcgcggg cggtgggcaa atcaccggcg tcaagcgcga gggcggcggc gaggcgctgg
     3001 cggtcggtga cgcgctgaaa gcgcgcgcca ccaggtatag cgctcgcata cggttcatcg
     3061 cgcacgtact gccaggctgg cgccgggtcg ccctgtgccc ggctcagcag cgccaggagc
     3121 cgttgcgcgc gaaacgtctc cggttccgcg cacgcctgcg cgcgggcgtc ctcccagtgc
     3181 ccctccagcc ataggatgtc gagcagcatc gtcccttccc agggcctgcg cgtgcccatc
     3241 gtctcggccg cgcgggcgaa cgcctgccgg gccgccacca gcaggtgctg gtaggccacc
     3301 cgctgatcag tctgataggg ccagacgatc cactggaact cgcccagcca catctgcccg
     3361 gtctcctggt actgcccggc ggcagcgaac gcggcgcggg cgtccgtgat ggcgcgccgg
     3421 gcctcgtcgg gttgtccgag cgcggcatag gtctgcgcca gccccgccag tccgcgcgcg
     3481 gttgttcccg ccgcgcgggc ggcaatcggc gccccggcgg cgagtgcctc ggcgtagcgg
     3541 cccgcgtgat tcagccagtt gacgagcgcc ccccggtgat aatcctcgtc gggccggata
     3601 ccctggatgc gcggcgcggg tagctgagcg cgcacggcga gcggcactga cgccaggctg
     3661 gcgaacgccg cctccaattg tggaagaccc tgggccaggt cgtccgtcag gcagcggaga
     3721 tgcgcctggt cgaagcgggc agcggttgcc aggagagcgt caccggcatc ctccgccagg
     3781 cacaccgcct ccgccgcact gatgctgccg cgctgtgggt cgctgtagcg gcgcaattgg
     3841 gccagcgtga gcagcgccac cgcacgctcg cgcggcgcga ggacgcccgt atccagcgcc
     3901 agcgccgcct cgtagcggtc ggcggcggtg agccaggcgt acgccatctg cgcccgctcg
     3961 ccggccgcga tcagccaggc cactgcttgc ggatcgccgg cttgctggaa gtggtaggcc
     4021 acggcgtccg ggtcgggcgc gggcgtggtc agcagcgcct cggctacctg ccggtgcagc
     4081 cggcggcggc gcggcggcag cacgcgctcg tacagcgcct cgcggacgag cgcgtgggcg
     4141 aagcgcagcc cgtcggcggt cggatcgagt agtcgcgccg ccgccgcgcg ctcgaccacc
     4201 gccagcagat cgtcctccgg cgcgccggcc accgcctgcc acagcgccag cggcgcgtcc
     4261 tgcccaatca cggcggcgat cgtcagcagg tcgcgcgccg ccgcgcccag gcggtccacg
     4321 cgcgcgtcga gcacctgg
//
LOCUS       DASUBI010000241         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1025094, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000241 DASUBI010000000
VERSION     DASUBI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025094"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFU72_04625"
     CDS             <1..213
                     /locus_tag="VFU72_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU5422804.1"
                     /translation="AASMLTEMMVGEPLEAVRELTAQELLDELGVPISPARVKCALLG
                     LKVLKSGAYGITDWPPQDAAHIGEEN"
     gene            226..543
                     /locus_tag="VFU72_04630"
     CDS             226..543
                     /locus_tag="VFU72_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5422805.1"
                     /translation="MASFSADEVREALKDVYDPEIGVNIVDLGLVYDVDVDDDDGKTD
                     VLITMTLTSMGCPLGPVIMQEVNRALGELPELGEIGVNLVWSPPWTPDMMSEEAKDEL
                     GIW"
     gene            1055..2359
                     /locus_tag="VFU72_04635"
     CDS             1055..2359
                     /locus_tag="VFU72_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEU5422806.1"
                     /translation="MVESTIHFTVPQSLDARFDLPDATSDGGLVWLAEAEQHLGLGAA
                     LAAALPEWRHGAIRHARATLIRQRIFQIACGYADQNDADTLRDDPLLQQVCGSDGPLA
                     SQPTLSRLENAVDRTACYRLSQALLQVYLQERERPGRPQHIRLDFDGTDDPVHGQQEG
                     SGYHGYYRQHMYFPLLVFDGDTDQLLAAVLRPGTVHAGHGALAVLKRLVRALRARWPG
                     VTIELRADSGFALPALYDYCEAEGLTYTIGLIPNTRLEALAADLMAEAERQQTETHAE
                     KVRLLAETTYQAGSWDHERRVVYKAEALPKGPNTRFVVTNRSDPPHDLYAWYVRRGTS
                     EQWIDDFKAACQADRLSDHRFWANQFRLLLHAAAYWLLDTLRRWLLQRGVPRCELHTL
                     QLLVLKIGGWVQRLTDRVRLHLAQSHPGQALWHLLATRSGRP"
     gene            complement(2428..2988)
                     /locus_tag="VFU72_04640"
                     /pseudo
     CDS             complement(2428..2988)
                     /locus_tag="VFU72_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
BASE COUNT          480 a         1060 c         1024 g          497 t
ORIGIN      
        1 gcggcttcga tgctgaccga gatgatggtc ggcgagccgc tggaggcggt gcgcgagctg
       61 acggcgcagg agctgctcga cgaattgggg gtgccgatca gcccggcgcg ggtcaagtgc
      121 gccctgctcg gcctcaaggt gctgaagtcg ggcgcctacg gcatcaccga ttggccgccg
      181 caggatgcgg cgcatattgg ggaagagaac tgacgggagc attgcatggc gagctttagc
      241 gccgatgagg tgcgcgaggc gctgaaagat gtctacgacc ccgagatcgg cgtcaacatc
      301 gtcgatctcg gattggtcta cgacgtcgac gtggatgatg acgacggcaa gaccgacgtc
      361 ctgatcacca tgaccctgac ctcgatgggc tgccctctcg ggccggtcat catgcaggag
      421 gtcaaccgcg ccctgggcga gctgcccgag ctgggagaga tcggcgtcaa cctcgtctgg
      481 agcccgccct ggactcccga catgatgtcc gaggaagcca aggacgagct ggggatctgg
      541 taacacgttc cattgcatga cgcggcccgg agtaccctcc gggccgcgtg cgtatagccg
      601 tgcatgccgc tcgagtgagc tcgaaacacc cgttcactga agcgccgcga gacgcttcca
      661 atgtgcgcgg cctcgacgcg cgcgtcggcc acgtccaggg atgggacggc gagccggcgt
      721 tggcctgacg gtcgctgcca gccgaggctg aggatgtctt cgctgttgcc cagttgccgg
      781 gcgttacctg ccagcgctct agcaacgagc gagacggccg atccgccgag tccattcttg
      841 ccggtcgtga ggcgcctgac cgaattcggc cagtgattct cggagagagc cgcggtggcg
      901 cccgaagcgg tcgacgctcc ctgtttggca ccaaagagga gccgctggct cggggaagct
      961 cagcggctag cctggattat tcatgaggtg acaggaaggc acctgtctgg tatcggtgaa
     1021 ggtgcttgat ccgcgccgag ccaggaggtg cctcatggtc gagtctacca ttcactttac
     1081 cgttccccag tccctcgacg cccgctttga tctgccggat gcgacctcgg acggcggtct
     1141 ggtctggttg gccgaagccg agcagcacct cggcctggga gccgccttgg cagccgccct
     1201 ccccgagtgg cggcatgggg ccatccgcca tgcccgcgcg accctgattc gccagcgcat
     1261 cttccagatt gcctgtggct atgccgacca gaacgacgcc gacacgctgc gggatgatcc
     1321 cctcttgcag caggtctgcg gcagcgatgg tcccttggcc agccagccga ccctgtcccg
     1381 gctggagaac gccgtcgatc gcacggcctg ctaccgcctg agccaggccc tgctccaggt
     1441 ctacctccag gaacgggagc gcccggggcg gccgcagcat atccggctcg attttgacgg
     1501 caccgacgat ccggtccatg gccagcagga agggagcggc taccacggct actaccgcca
     1561 gcacatgtac ttccccctgt tggtcttcga tggggacacg gaccagctcc tcgcggcggt
     1621 cctgcggccc ggcacggtcc acgccgggca tggtgcgctg gccgtgctca aacggctggt
     1681 gcgggcgcta cgagctcgct ggccgggcgt caccattgag ctgcgcgccg acagcggctt
     1741 cgcgctgccg gccctctacg actactgcga ggccgaaggg ctcacctaca ccatcggcct
     1801 gatccccaat acgcgcctgg aagccttggc ggccgacctc atggcggagg ccgagcgcca
     1861 acagacggag acccacgccg agaaggtgcg cttgctggcc gagaccacct atcaggccgg
     1921 ctcgtgggac cacgagcgcc gcgtcgtcta taaggccgaa gcgctgccca agggcccgaa
     1981 tacccgcttc gtggtcacga accgcagcga tccgccccac gacctctacg cctggtacgt
     2041 ccgccggggc accagcgagc aatggatcga cgacttcaaa gccgcctgcc aggccgatcg
     2101 cttgagtgac catcgcttct gggccaacca gtttcgcctg ctcctgcatg ccgccgccta
     2161 ctggctgctc gataccctgc gccgctggct gctgcagcgg ggagtcccgc gctgcgagct
     2221 ccacaccctc cagttactgg tgctcaagat cggcggctgg gtccagcgcc tgaccgaccg
     2281 agtccgtctg catctggccc aaagccatcc ggggcaagcc ctctggcacc tcttggccac
     2341 ccgctcaggg cgtccatgaa taatccgggc tagggcgcgg atcatctcgt cgctgagctg
     2401 gcgatcgccg gggaccagcg cgcgacagtt ggagtggctg gccagcacgg taccatggaa
     2461 gcgctccagc gcctcccaga agctctggtc ggtcaggtgg ctgaggtcga ggatcaggcc
     2521 gagccgctcc atctcggcca gcagcgcgcg cccctcgtcg ctcagcggcc cggtggactc
     2581 ggtgcccttg gcgtaggcgc tcggcccata gtgcgccagc ccgaccacgc gcaggccgtc
     2641 gtcccaccac gcccgcgcct gctccggcgc gacgatcggg tcggcgccct ccatgcagag
     2701 gatgatgccg agcggggtcg tcgccgggtc cgcacgccag tcgtcgagct gccgctgcag
     2761 tgccgcccag tcggtgagca ggcgcagcac cccctggcgc tccagttcgc ggtagtacgc
     2821 aagctgcccc tgcgcctggg cgtaggcgat ctcgtgggtg cggtagccgg gcagccggct
     2881 ctcgggctgc tgcacgcgcg cgatgaccgt ggccagcgag acgaccgcct cggcccggcg
     2941 cagggccggg aaggagaccg tgcccgcggc gcgccccttc tcggtcatcc cctctcccgc
     3001 ctcctgcgcg cgaatctcgt gcacgccgcg gcgcaggtcg cgattccagt tgagcgcgtt
     3061 c
//
LOCUS       DASUBI010000242         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1080110, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000242 DASUBI010000000
VERSION     DASUBI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080110"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFU72_04645"
     CDS             complement(<1..400)
                     /locus_tag="VFU72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEU5422807.1"
                     /translation="MARVDRHAGDDEIDFRVRKPNRARRPERRELDHDHDVTAERRTR
                     RAPEADTFRCKHCKRLVGPTLSGGRHRNHCPVCLYSRHVDGKTPGDRASDCRSLMEPV
                     GLFARPSGEQVVVHRCLGCGLERYNRVAADD"
     gene            982..>2410
                     /locus_tag="VFU72_04650"
     CDS             982..>2410
                     /locus_tag="VFU72_04650"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5422808.1"
                     /translation="MEIEFRPGALVEQEVDTLIIPVGPGQDLGDEAAAVNRRLEGALA
                     RLLADRDFKGAIGDTLVVPTFDRLPARQLVLTGVGAPERRGAGEITRAWSVAARAARE
                     AGGQVVASGQPPAGQLGAASAARAAVEGVILGLYRFLEYKTGPAPARAIETLVFTGDD
                     AAVKRGVALGEAVAAGVVLARNLGNQPPNALYPESLAAGAAELAQVRGLQVKIYGPDE
                     LEELGAGAILAVGKGSVHEPRLIHLTYNPQGASQGSIALIGKSITFDSGGINLKPSGE
                     GITSMKIDMAGGGAVLGAMSVVPSLGLPFTVHGVLAAAENMPSAGSFRPGDILTTMSG
                     KTVEIGSTDAEGRLVLADALTYSIGQGARSLVDLATLTGSNVVALGDQGTGVYGNDQG
                     LVDEVLAAGGAVGELLWQMPLWEVYEGRLKGDFADLRNTSTRDGGAIFAALFIREFSQ
                     GVPWAHLDIAGPSWTSRGTDLSTAGA"
BASE COUNT          361 a          822 c          894 g          333 t
ORIGIN      
        1 tgtcgtcggc ggcaacccgg ttgtagcgct ctaggccgca gccgaggcag cggtggacga
       61 ccacctgctc cccggacggc cgggcgaaga gtccgaccgg ctccatcagc gagcggcagt
      121 cgctggcgcg gtcgccgggc gtcttgccgt cgacgtggcg cgagtagagg cagaccggac
      181 agtggttgcg gtggcggccg ccgctgagag tcgggccgac cagccgcttg cagtgcttgc
      241 agcgaaaggt gtctgcctcc ggcgcgcgcc gcgtgcgccg ctcggcggtc acatcgtggt
      301 cgtggtccag ttcacgccgc tccgggcggc gcgcccggtt cggcttacgg acgcggaaat
      361 cgatctcgtc gtcgcctgcg tggcggtcta cgcgtgccaa tggggtctcc ttcaatcagc
      421 aacaaggccg gtagctggag ctgctggaaa aaacccgtgg acgcgtatac cctgggtgac
      481 atggtgctcc tccatctgac acaaaaaacg gacaggtcaa gacaatcccg gcgaggccgc
      541 cgccggcaag accggtgacg acagccgggt acgcaaagcc gcggcggggg cgcccgcgtt
      601 agctcgtgtg ctcgctgttg gatctagtca gatggtgaat cagacgagga gacgacgagc
      661 gcgagcaggc accggaacgg ccgcggcgtg gcggacagcc gcttgatgtc gcttcatggc
      721 cccatgccct tctagcccga tcgcgtgccg cacggcgcgg caccgcacca cgatagcatc
      781 cgtcctcaat cctcgtcaag catcgcgcgg aatggtcggg cgaccaatcg gtcgggagac
      841 gatggccgtg gattcggagc ctcgctgcct ctcgacgccg gctacactcc tgacccatac
      901 tgcgacccac ggcagcggct ggatcaccgc cgcgcccgcg aatgccaccg cgatacggtc
      961 gatgcgagga gagggagcat catggagatc gaatttcgcc ccggcgcgct ggtcgagcag
     1021 gaggtcgaca cgctgatcat tccggtcggg cccggccagg atctgggcga cgaggccgcc
     1081 gcggtcaatc gccgcctgga gggcgcgctg gcgcgcctgc tggccgaccg cgacttcaag
     1141 ggcgccatcg gcgacacgct ggtcgtgccg accttcgacc gcctgccggc ccgccagctg
     1201 gtgctgaccg gtgtcggcgc gcccgagcgg cgcggcgcgg gggagatcac ccgcgcctgg
     1261 tcggtggcgg cccgcgcggc gcgcgaggcc ggagggcagg tcgtggccag cggccagccg
     1321 cccgccgggc agctcggcgc cgcgagcgcg gcccgcgcgg cggtggaggg ggtgatcctc
     1381 ggcctctacc gcttcctgga gtacaagacc ggtccggcgc cggcgcgggc gatcgagacc
     1441 ctggtcttta ccggcgacga tgccgccgtg aagcgcggcg tggcgctggg cgaggccgtg
     1501 gccgcggggg tggtgctggc gcgcaacctc ggcaaccagc cgccgaacgc gctctacccc
     1561 gagtcactgg ccgcgggggc cgccgagctg gcgcaggtgc gcgggctgca ggtcaagatc
     1621 tacggcccgg atgaactgga ggagctgggc gccggggcga tcctggcggt cggcaagggg
     1681 agcgtgcacg agccgcgcct gatccacctg acctacaacc cccagggcgc ttcccagggc
     1741 tcgatcgcgc tgatcggcaa gtcgatcacc ttcgactcgg gcggcatcaa cctcaagccc
     1801 tcgggcgagg ggatcaccag catgaagatc gacatggcgg gcggcggggc cgtactgggc
     1861 gcgatgagcg tcgtaccctc gctcgggctg ccgttcaccg tgcacggcgt gctggccgcg
     1921 gccgagaaca tgcccagcgc cggcagcttc cgccccggcg acatcctgac cacgatgagc
     1981 ggcaagacgg tcgagatcgg cagcaccgat gccgagggac ggctggtgct ggccgacgcg
     2041 ctgacctaca gcatcggtca gggggcgcgc tcgctggtcg atctggccac cctgaccggc
     2101 tcgaacgtgg tggcgctggg cgaccagggg accggggtct acggtaacga ccaggggctg
     2161 gtcgacgagg tgctggcggc gggcggcgcc gtgggcgagc tgctctggca gatgccgctc
     2221 tgggaggtct acgagggccg gctgaagggc gacttcgcgg acctgcgcaa cacctcgacc
     2281 cgcgacggcg gcgccatctt cgccgcgctc ttcatccgcg agttcagcca gggcgtgccc
     2341 tgggcgcacc tggacatcgc cggtccctcg tggaccagcc gcggcaccga cctgagcacc
     2401 gccggcgcca
//
LOCUS       DASUBI010000243         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_751060, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000243 DASUBI010000000
VERSION     DASUBI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751060"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFU72_04655"
     CDS             complement(<1..445)
                     /locus_tag="VFU72_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5422809.1"
                     /translation="MTDYCPTCGGDLVSGQQCPLCAALQPIEAIDNAAAAIPAADVPA
                     AAFDQDAAAYERYLDRGRGRLRQELAVGNLLEALPDLSRRRRRVLDLGGGGGRLAVRL
                     ARLGHEIDLIDPSPEMVRLARLRAHGAGVDESVTCTLGSAEDLAGG"
     gene            complement(450..1484)
                     /locus_tag="VFU72_04660"
     CDS             complement(450..1484)
                     /locus_tag="VFU72_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5422810.1"
                     /translation="MAVAVGIGLTNECNLACPHCYRPTLALQRLTLAQVETILDNLDV
                     GSVNLGVGENGVHPEYRQMLALLRARGVKTAITSNGYSLDLLDDEELCGFHSVELSFD
                     FPTKAEMDQFRGEGSWEMAIESLERCQRLGIPTAVTGVMMNSNFHRMGELAAFAFSLG
                     CDLRVNVYQPVTGEGFTLSYEQFWQGFANLFAAGPVVVCSEPLVNALMGLETTRGNAC
                     GRRSIRALPAGTIAPCPYWPVAAGRIRQLAELGDAIWETREFARARQVPTACQDCRYV
                     ESCAGGCASRRALRGRLDEPDEYCPVVRGEAEVARIQQLLRYSLSDPEDLPKAGNACT
                     TVIRASARVG"
     gene            1747..>2324
                     /locus_tag="VFU72_04665"
     CDS             1747..>2324
                     /locus_tag="VFU72_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5422811.1"
                     /translation="MGIVNLGILAHVDAGKTTLTERILFETGIIRAVGSVDQGTTQTD
                     TLELERARGISIKSAVVSFQLGQLKVNLIDTPGHADVVAEVARSLDVLDAVVLVVSAV
                     EGVQPQTRRLARAIRAARLPLLIFVNKIDRLGARGDAILPELRTKLRLRAVPLNAATG
                     PGERAARVVSLDRDDPVWREAVLDLLAETSEQV"
BASE COUNT          367 a          833 c          775 g          349 t
ORIGIN      
        1 ggccgccggc cagatcttcg gcgctgccga gcgtacaggt gacggactcg tccacgcccg
       61 cgccgtgcgc gcgcagcctg gccaggcgga ccatctccgg cgagggatcg atcagatcga
      121 tctcgtgtcc cagccgcgcc agccgcacgg ccaaccggcc accgccgccg cccagatcga
      181 gcacgcgccg ccggcgccgc gacaggtcgg gcaatgcctc cagcagattc ccgacggcca
      241 gttcctggcg caggcgcccc cgtcccctgt cgaggtagcg ctcgtaggcc gcggcgtcct
      301 ggtcgaacgc ggccgctggc acgtcagcgg cgggaatcgc ggccgccgcg ttgtcgatgg
      361 cctctatcgg ttgcagcgcc gcgcagagcg ggcactgttg accggaaacg aggtcgccgc
      421 cgcaggtggg gcagtagtcc gtcacggcct tagcccacgc gagccgacgc acgaatcact
      481 gtcgtgcagg cgttgccggc cttgggcaga tcctccgggt cgctgagcga atagcgcagc
      541 agctgctgga tgcgcgcgac ctccgcctca ccgcgcacca ccgggcagta ctcgtccggc
      601 tcatcgagcc ggccgcgcag cgcccgccgg ctggcgcagc cgcccgcaca gctctcgaca
      661 taacgacaat cctgacaggc ggtgggcacc tgccgcgcgc gggcgaactc gcgcgtctcc
      721 cagatcgcgt cgcccagctc ggccaattgc cggatgcggc ccgccgctac cggccagtag
      781 gggcaggggg cgatggttcc ggccggcagc gctcggatgc tgcgccttcc gcaggcattc
      841 ccccgcgtgg tctccagccc catgagcgcg ttgaccagcg gctcgctgca gacgaccacc
      901 ggcccggcgg cgaagagatt ggcgaagccc tgccagaact gctcgtagct gagggtgaag
      961 ccctcgccgg tcaccggctg atagacgttg acgcgcagat cgcagccgag gctgaaggcg
     1021 aatgcggcca actcgcccat gcggtggaag ttgctattca tcatcacgcc ggtgaccgcg
     1081 gtcgggatgc cgagccgctg gcagcgctcc agcgactcga tcgccatctc ccaggagcct
     1141 tcaccgcgga actggtccat ctccgccttg gtggggaagt cgaagctcag ctcaaccgag
     1201 tggaagccgc acagctcctc gtcgtcgagc aggtccaggc tgtagccgtt ggaggtgata
     1261 gctgtcttga cgccgcgcgc gcgcagcagc gccagcatct ggcggtattc ggggtggacg
     1321 ccgttctcac ccacaccgag attgaccgag ccgacgtcca ggttgtcgag gatcgtctcg
     1381 acctgcgcca gtgtcagccg ctgcagcgcc agcgtcgggc ggtagcagtg cgggcaggcc
     1441 aggttgcact cgttggtgag cccgataccg accgcgaccg ccattgacgc ctccctgact
     1501 gaacccggca ccatcatcgc gcgacgacag gatacccgca tccgcccgcg acgtatacgc
     1561 gccggacggc agcacggacg ttcgcgcgcc gggcgcccat cccgcccgct ggtctataat
     1621 ggcgactgat cggcccgccg tgcgggccga atggtttcgg cgcacatgtc aggcacgagg
     1681 agcgtcccat ggggcagacg ttgaagaacg cagccgtcat ctgtctcttc agcgaaggcc
     1741 atctgcatgg gaatcgtcaa tctcgggatc ctggcgcatg tcgacgccgg taagacgacc
     1801 ctcaccgagc gcatcctctt cgagaccggc atcatccgcg cggtcggcag cgtcgaccaa
     1861 ggcaccacac agaccgacac gctggagcta gagcgcgctc gcggtatctc gatcaagtcc
     1921 gcggtcgtct cgttccagct cggacagctc aaggtcaatc tgatcgatac ccccggccac
     1981 gccgacgtcg tggccgaggt ggcgcgctcg ctcgacgtgc tcgacgccgt cgtgctggtg
     2041 gtctccgccg tcgagggggt gcagccgcag acgcgccgcc tggcgcgagc catccgcgcc
     2101 gcccgtctgc cgctcctgat cttcgtcaac aagatcgacc gtctcggagc gcgcggcgac
     2161 gctatcttgc ccgagctgcg caccaagctg cgtctgcgcg ccgtaccgtt gaacgccgcg
     2221 acagggccag gcgaacgtgc cgcgcgggtc gtttcgctcg accgcgatga cccggtttgg
     2281 cgcgaggcgg tgctcgacct gctggccgag acgagcgagc aggt
//
LOCUS       DASUBI010000244         5718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_344163, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000244 DASUBI010000000
VERSION     DASUBI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344163"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..898)
                     /gene="otnI"
                     /locus_tag="VFU72_04670"
     CDS             complement(113..898)
                     /gene="otnI"
                     /locus_tag="VFU72_04670"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF043033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEU5422812.1"
                     /translation="MPRFAANLTMLFTEYPFLERFDRAAAAGFQAVEFLFPYDQDVPA
                     IAAALRRNRLAQVNFNLPAGDFAGGERGIANDPHRVDAFRDGVERAIAIAGELGCLRL
                     NCLVGLALPEVPIALQWDVLTDNLRFAAERTAEAGITLLVEPLNNLDTPGYLLVHTDQ
                     ALELLDRAGNPNLKLQYDCYHMQRMEGNLTATLVEHLARIGHIQIAACPGRHQPGSGE
                     INYPFIFEALDAAGYDGWVSLEYNPVPNTEASLAWLRDWGYWT"
     gene            1037..1825
                     /locus_tag="VFU72_04675"
     CDS             1037..1825
                     /locus_tag="VFU72_04675"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEU5422813.1"
                     /translation="MAGGAEPQRVGLIGLGAIGRSLANLAAGRDDLALVGALVRDTRK
                     DRGVAVPVVSTLAELLALGPEVVVEIAGHEGLRQHGAAALRAGYDLIAVSVGALADPA
                     LQDELLEAAQASGRQVKVASGAIGALDAIAAARLGGLERVTHTTRKPATTLLGAEGAT
                     LREPRELFRGPARAAVVKFPESVNVAAAVSLAGLGLDRTEVCVIADPAVERNQHVVEV
                     EGAFGRLRFEIAGIPSGDNPKTGRIVAMSILHTILMRRAPLVVG"
     gene            1901..1973
                     /locus_tag="VFU72_04680"
     tRNA            1901..1973
                     /locus_tag="VFU72_04680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1934..1936,aa:Lys,seq:ttt)
     gene            complement(2062..2751)
                     /locus_tag="VFU72_04685"
     CDS             complement(2062..2751)
                     /locus_tag="VFU72_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5422814.1"
                     /translation="MSVFVLVHGSFSGGWKWKKVVPLLRQAGHQVSTPTLTGLGERSH
                     LVWPGLGLETHITDIVNVLRFNDLEDVILAGHSWGGMVITGVAERVPERIARLVYVDA
                     FVPHDGESVFSISPAAGERWRARTVAGMTQPYEPEHWNISDPEDAAWLQAHMVPMSIK
                     THEDPIALPAYRAAELPRTYIWCTESAAFAPMARRARDEGMRYFELPTHHNPMVTMPR
                     ELTDILLTSAE"
     gene            complement(2798..3565)
                     /locus_tag="VFU72_04690"
     CDS             complement(2798..3565)
                     /locus_tag="VFU72_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5422815.1"
                     /translation="MANGPVLWAKLSWPEIEDLVRRGPRMAIWPLGTTEEHGRHLPVD
                     VDLANCWEVARGVSANTGVPLIPPLPYGDSGFWRGWPGTLSLSAESFIAVVGDVCAGL
                     YESGIRKILLLNGHLGNVPALRIASRKVQARHPDAQVRALSWWDVSPRVIEVMYADSI
                     TGASRSFHGNDGETSVYLTHSPELVHLDLAVDEPDRSRPQPFFTYGSQQITGSGAIGH
                     PTSASVDKGREVLRWAIEDLTAEVRRAIEEIAPGVRS"
     gene            complement(3621..4379)
                     /locus_tag="VFU72_04695"
     CDS             complement(3621..4379)
                     /locus_tag="VFU72_04695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU5422816.1"
                     /translation="MGTRVLDGKVALITGAGRGFGRAIAERFAEEGADLALNYRASRA
                     GCEEVAAATRERGQRATLAQADVSDGAAVAAMVRQVVEELGRIDILVNNAGIMRLGPF
                     EQSGEQPWQEEIATNIFGPLQVTRAVVPHMIARRYGKIVNLSSQLAITGWERGAVYAG
                     TKGFILTWTRSLARELGQYNINVNAIGPGSILTDMNRSVYPDEAAIQRRAQELPLRRL
                     GTPRDVAEVALYFASDAASFLTGQFLSPSGGNVM"
     gene            complement(4562..5557)
                     /locus_tag="VFU72_04700"
     CDS             complement(4562..5557)
                     /locus_tag="VFU72_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5422817.1"
                     /translation="MVIDLHSHYFPLEAAGQPGAPVTVAEGGEPTRLVVAGHAMSLDA
                     GLFDLARQRDDLQRQGLTRRVLAVPPFTILYELPAAEGLRWSRALNDSLARAQARDSE
                     RFIGFATVPLQDAEAAAQELERASGELGLQGVEILTSVNGVGLDTPALDRFWRAAERV
                     GLPILVHPHYVSGAERMGDYHLRNLIGNPAETAQAGARLLFGGVIERFPGLKLVLSHG
                     GGALPHLIGRLRQGYAVRAETRARASDPLAGLTRLYYDTVVFDPTVLRHLVELVGASQ
                     VVVGTDYPFDMSEGRPVDLVRAAGLGQAEQETILQAAERLLPTGVAGRASGSQGV"
BASE COUNT          837 a         1959 c         2073 g          849 t
ORIGIN      
        1 ccccggcccc tctcccaata ctgggagagg ggagtacgaa gtgctcctgt tacgtactcc
       61 cccttcttcc agtattggga gaagggggtt gggggatgag ggctcctccc ggttatgtcc
      121 agtagcccca gtcgcgcagc caggcgagcg acgcctcggt gttgggcacc gggttgtact
      181 ccaggctgac ccagccatcg tagccggcgg catcgagcgc ctcgaagatg aaggggtagt
      241 tgatctcacc gctgccgggc tgatggcggc ccgggcaggc ggcgatctgg atgtgcccga
      301 tgcgcgccag gtgctcgacc agtgtggcgg tgaggttccc ctccatgcgc tgcatgtggt
      361 agcagtcgta ttgcagcttc aggttgggat tgccggcgcg gtcgagcagc tccagcgcct
      421 ggtcggtgtg gaccagcagg tagccgggcg tgtcgaggtt gttgagcggc tcgaccagta
      481 aggtgatgcc cgcctcggcg gtgcgttcgg cggcgaagcg caggttgtcg gtcagcacgt
      541 cccactgcaa cgcgatcggc acctccggca gcgccaaccc gaccaggcag ttgagccgga
      601 ggcagccgag ctccccggcg atggcgatgg cgcgctcgac gccgtcgcgg aaggcgtcga
      661 cgcggtgtgg gtcgttggcg atgccacgct cgccgccggc gaagtcgccg gccggcaggt
      721 tgaagttgac ctgcgccagc cggttgcgcc gcagcgcggc ggcgatggcc ggcacgtcct
      781 ggtcgtaggg gaagaggaac tcgaccgcct ggaagccggc cgccgcggcg cggtcgaagc
      841 gctccagaaa cggatactcg gtgaagagca tggtcagatt ggcggcgaaa cggggcatgg
      901 tgcctccctc atctccgtcc ggcgcgtgcg gctgccgcgc ggagcggctg ccgggaaggt
      961 agcccaggcg gcggcggggc gcaagccgag gcgatcgggt aagatgcggc gacgggcgca
     1021 caagaggagg tgggtgatgg cagggggagc agagccacag cgggtggggc tgatcggcct
     1081 gggcgcgatc ggccgctcgc tggccaacct ggcggcgggc cgcgacgacc tggcgctggt
     1141 cggggcgctg gtgcgcgaca ccaggaaaga tcgcggggtg gcggtgccgg tggtgagcac
     1201 gctggcggag ctgctggcgc tgggaccgga ggtcgtggtc gagatcgccg ggcacgaggg
     1261 attgcgccag cacggggcgg cggcgctgcg ggccggctac gacctgatcg cggtcagcgt
     1321 gggcgcgctg gccgacccgg cgctgcagga cgaactgctt gaggcggcgc aggcgagcgg
     1381 gcggcaggtc aaggtggcgt cgggggcgat cggggcgctg gacgcgatcg cggcggcgcg
     1441 gctgggcggg ctggagcggg tgacgcacac cacacgcaag ccggccacga cgctgctggg
     1501 tgccgagggc gcgacgctgc gcgagccgcg cgagctcttt cgcggcccgg cgcgcgcggc
     1561 ggtggtgaag ttcccggaga gcgtcaacgt ggcggcggcg gtcagcctgg ccgggctcgg
     1621 gctggaccgg accgaggtct gcgtcatcgc cgacccggcg gtcgagcgca accagcacgt
     1681 ggtggaggtg gaaggcgcct tcggtcggct gcgcttcgag atcgcgggga tcccctcggg
     1741 cgacaacccc aagaccggcc gcatcgtggc catgagcatc ctgcacacca tcctgatgcg
     1801 ccgcgcgccg ctggtagtcg gctagcgcgt ccgcccccac tgaacgctgc cctgtcacgc
     1861 tcggcaggat tctcgtagaa tcaatgaaga gccagcccac gagcccgtag ctcagctggc
     1921 agagcagcgg acttttaatc cgcgggtcct gggttcgaat cccagcgggc tcactctcga
     1981 tatatagccc acgcgcccga gggcgcgtgc gccgcaccgt gtcgcaggtg ccttcccggt
     2041 cgacggagcg aagctccggc gctactcggc gctcgtcagc aggatgtcgg tcagctcgcg
     2101 cggcatcgtg accatggggt tgtggtgggt cggcagctcg aagtagcgca tgccctcgtc
     2161 gcgcgcgcgc cgcgccatcg gggcgaacgc ggccgactcg gtgcaccaga tgtaggtccg
     2221 cggcagctcg gcggcccggt acgcgggcag cgcgatcggg tcctcgtgcg tcttgatcga
     2281 catggggacc atgtgcgcct gcagccaggc cgcgtcctcg ggatcgctga tgttccagtg
     2341 ctctggctcg tacggctgcg tcatcccggc gacggtgcgc gcccgccagc gctcgcccgc
     2401 cgccggactg atgctgaaga cgctctcgcc gtcgtgcggc acgaaggcgt cgacgtagac
     2461 cagccgggcg atgcgctcgg gcacgcgctc cgccacgccg gtgatgacca tgccgcccca
     2521 gctgtggccg gccaggatca cgtcttccag atcgttgaag cgcagcacgt tgacgatatc
     2581 ggtgatgtgg gtctccaggc ccagacccgg ccagaccaga tggctgcgct cgcccagccc
     2641 cgtcagcgtc ggcgtggaga cctgatgtcc cgcctgccgc aagagcggca ccaccttctt
     2701 ccacttccag ccgccggaga acgagccgtg caccagtacg aagacgctca tcgatgtgcc
     2761 tctctctgct tgggcgtgcg cgttccagat gaccgcctca ggagcgaacc ccgggcgcga
     2821 tctcctcgat agcgcgacgc acctccgccg tcaggtcttc gatcgcccag cgcaggacct
     2881 cgcggccctt gtcgacactc gcgctcgtcg ggtggccgat cgcgccgctt ccggtgatct
     2941 gctgcgagcc gtaggtgaag aagggctgcg gccgcgagcg gtccggctcg tcgaccgcca
     3001 ggtcgagatg caccagctcc ggcgagtggg tcagatagac cgaggtctcg ccgtcgttgc
     3061 cgtggaagct gcggctggcg ccggtgatgc tgtccgcgta catgacctcg atgacgcgcg
     3121 gcgagacatc ccaccaactg agggcgcgca cctgcgcgtc cgggtggcgc gcctgcacct
     3181 tacgcgaggc gatgcgcagc gcgggcacat tgcccaggtg cccgttcaag agcagtatct
     3241 tgcggatgcc gctctcgtac agcccggcgc agacatcgcc gaccacggcg atgaagctct
     3301 cggcgctcag cgacagcgtg ccgggccagc cgcgccagaa gcccgagtcg ccgtagggga
     3361 gcggcgggat cagcggcacg ccggtgttgg ccgagacccc gcgcgcgacc tcccagcagt
     3421 tggccaggtc gacgtcgacc ggcaggtgcc ggccgtgctc ctcggtggtg ccgagcggcc
     3481 agatcgccat gcgcggcccg cgccgcacca gatcctcgat ctcgggccag gagagcttgg
     3541 cccagagcac ggggccattc gccatgacag tacctccagc atctcgacga gtcgcgcagg
     3601 gcgcgctggt tgagcggcgt ctacatcacg ttgcccccgc tggggctgag gaactggccg
     3661 gtcaggaagc tggcggcgtc cgaggcgaaa tagagggcca cctcggccac gtcgcgcggc
     3721 gtgcccagcc ggcgcagcgg cagctcctgg gcgcggcgct gaatagccgc ctcgtcgggg
     3781 tagaccgagc ggttcatgtc ggtcaggatc gagccggggc cgatggcgtt gacgttgatg
     3841 ttgtactgcc ccagctcgcg cgccagcgag cgggtccagg tcaggatgaa gcccttggtg
     3901 ccggcgtaga ccgcgccccg ctcccagccg gtgatcgcca gctgcgacga gaggttgacg
     3961 atcttgccgt agcgccgcgc gatcatgtgg ggcacgaccg cgcgcgtcac ctgcagcggc
     4021 ccgaagatgt tggtggcgat ctcttcctgc cagggctgct cacccgactg ctcgaacgga
     4081 cccagccgca tgatgccggc gttgttgacc aggatgtcga tgcgccccag ctcctcgacc
     4141 acctgccgca ccatggccgc caccgccgcg ccgtcgctga cgtcggcctg cgccagcgtc
     4201 gcgcgctgcc cgcgctcgcg cgtcgcggcg gcgacctcct cgcagcccgc ccggctcgca
     4261 cgatagttga gcgccagatc ggcgccctcc tcggcgaagc gctcggcgat cgcgcgcccg
     4321 aagccgcgcc ccgcgccggt gattagcgcc accttgccgt caagtactct cgtacccata
     4381 ctgcacgctc ccgtcacacg ctgccagcga ctggataggc cggggatgct ggcgttcgag
     4441 ggaaagccgt cctacctcgc gtcgctctct tcagcctgcg tgagcaggct tcgctctcga
     4501 gcgcggcggc ttcagctcgg acggagccgc gcggtcccgc ggcgacgacc gattcacgca
     4561 gtcaaactcc ttggctcccc gacgcacgcc cagcgacgcc ggtcggcagg aggcgctccg
     4621 cggcctgaag aatcgtctcc tgctcggcct ggcccaaacc ggccgcgcgc accaggtcga
     4681 ccggccggcc ctcggacatg tcgaacggat aatccgtccc caccaccacc tgcgaggcgc
     4741 cgaccagctc gaccaggtgg cgcagcacgg tcgggtcgaa gacgacggtg tcgtagtaga
     4801 ggcgcgtcag cccggccaac ggatcgctgg cgcgggcgcg agtctccgct cgcaccgcgt
     4861 acccctgccg cagccggcca atgaggtgcg gcaacgcgcc gccgccgtgc gagaggacca
     4921 gcttgagccc cgggaagcgc tcgatgaccc caccgaagag gaggcgcgcg cccgcctgcg
     4981 cggtctcggc cggattgccg atcaaattgc gcagatggta gtcgcccatg cgctcggccc
     5041 cggagacgta gtgggggtgg accaggatgg gcaggcccac gcgctcggcc gcgcgccaga
     5101 agcgatcgag cgcgggcgtg tccagcccga cgccgttgac gctggtgagg atctcgacgc
     5161 cctgcaagcc cagctcgccg ctggcacgct ccagctcctg cgcggcggcc tccgcgtcct
     5221 gcagcggcac cgtggcgaag ccgatgaagc gctcggagtc ccgcgcctgt gcccgcgcca
     5281 ggctgtcatt gagcgcacgt gaccagcgca gcccctcggc ggccggcagc tcgtagagga
     5341 tggtgaaggg cggcacggcg agcacgcgcc gcgtgaggcc ctggcgctgg aggtcgtcgc
     5401 gctggcgcgc gaggtcgaag agaccggcgt ccaggctcat cgcgtgcccg gcaaccacca
     5461 gccgggtcgg ctcgccgccc tcggcgaccg tcaccggcgc gcccggctgc ccggccgcct
     5521 ccagcgggaa gtagtgactg tgcagatcga tgaccaaatt actctcctgt gctgtgaccc
     5581 tgctcgctgg catatacgac ggcgggttat gacctttgac acaatacttc ggcccgactc
     5641 ggaagccttc tcagcctgct ttcagcaggc ttcgcttgct agcgcggtgg cttcagcccg
     5701 gcgcggtcgt gcggtctg
//
LOCUS       DASUBI010000245         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_736852, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000245 DASUBI010000000
VERSION     DASUBI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736852"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFU72_04705"
     CDS             complement(<1..448)
                     /locus_tag="VFU72_04705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5422818.1"
                     /translation="MGNRGGMEQGTGQNQAGHATEPARQPSSTTAFWQHYYATPAPAP
                     GARPRLPYLPALDGIRGVAVLAVIVYHARPGWLPAGFLGVDIFFVLSGYLITGLLMAE
                     WERHQAIDLPAFWLRRARRLLPALYLLLLVALAYAVVFRPANLAQLR"
     gene            complement(588..2504)
                     /locus_tag="VFU72_04710"
     CDS             complement(588..2504)
                     /locus_tag="VFU72_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5422819.1"
                     /translation="MPAREDLAELIVSLSFPEDPRIAPGGRRVAYVATPYGREKDAEG
                     AIWLAPLDGGTPARQITQGNGRDERPRWSPDGQSLAFLSERVKPGIAALYRMPLSGGE
                     PEPLVERKRGIASFAWSPDGRRIAFLAPDEPTEEDERREKERDDAEVYGERWPLARLH
                     LLDLASGAVTTLPTGEAHLAELAWSPDGARIAFSAWRTPEIESHLRARIATIAVEHPE
                     QQTTVVDEANGWSAHQLTWSDDGAKLLFVGSHDPEPQGSFTLYAVDATGGRPRVLGPS
                     ADEECCVTGLKAVPGSARVVISVAEGLSTRLEWVDTESGAREPLYAPRDGDISSFDVR
                     VVDGAVKLAVVESAGDRPPELYGGTPGQLQPLSAHHAALGEFEFGRQEPFHWTAPDGL
                     PLDGVLIRPPQAPDGPLPLVVLVHGGPYGRSSNGWNLRPLNWAQWLAAHGYAVLLPNY
                     RGGMGHGNAFAAHARGNVGEGDFPDVLSGVDAAIERGIADPARLGIGGWSQGGFMTAW
                     AVTQTSRFKAGVMGAGVSDWGMMTMTSDMPTFESVLGGDRPWDGPGPHRAAAISPISY
                     AGRVETPLLMLHGKYDERVPVTQAIGYERALRERGVPVQLVVYPREPHGVRERAHQRD
                     ILRRVVAWYDRWLS"
     gene            2645..>3214
                     /locus_tag="VFU72_04715"
     CDS             2645..>3214
                     /locus_tag="VFU72_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5422820.1"
                     /translation="MRRSVALLAVIIALQVGLAGWLLADHRSGAAGARLAPDLTVVTP
                     SHWRPATLGRQLTVMTYNLQEGFSRDDRFDLERQAAAIAAERPDVVVVQEVSRGWLVA
                     AGADEVLWLSQRLGMQAIFGANSDDGLWGNAILSRAPLTDAAREQYTSTQNLKRSVVS
                     ARVETAAGPLWIYGTHLDNPVGATQVRLDQ"
BASE COUNT          444 a         1185 c         1147 g          438 t
ORIGIN      
        1 gccgcagttg ggccagattc gccggccgaa atacgaccgc gtaggccaac gcgaccagca
       61 gcagcaggta gagcgccggc agcaggcgcc gcgcccggcg caaccagaag gccggcaggt
      121 caatcgcctg gtggcgttcc cattcggcca tgaggagtcc cgtgatcagg tagccgctca
      181 gcacgaagaa gatgtcgacg cccaggaagc ccgccggcag ccagcccggg cgcgcgtggt
      241 agacgatcac cgccaggacg gccacgcccc ggatcccgtc cagcgccggc agatagggca
      301 gccgcggccg cgcgcccggg gcgggcgcgg gggtcgcgta gtagtgctgc cagaatgcgg
      361 tggtggagga cggctggcgt gccggttcgg tggcgtgtcc cgcctggttc tgcccggttc
      421 cctgctccat cccgccgcga ttccccatga ttcacaccga gcgctggtcg tgccgcgtcg
      481 cccggggcgc tccctcaccg tcgcgactgc tccggctgcc acggacagtg gccactacgc
      541 actcaagtgc gcgagggcgg acgaacgtca caactgcgac tggccgtcta cgatagccag
      601 cggtcgtacc acgcgaccac gcgacgcagg atgtcgcgct ggtgggcgcg ttcgcgcacg
      661 ccgtgcggct cgcgcgggta gacgacgagc tgcaccggca cgccgcgctc gcgcagcgcc
      721 cgctcgtagc cgatcgcctg cgtcaccggc acgcgctcgt cgtacttgcc gtgcagcatc
      781 aagagcggcg tctcgacccg cccggcgtag gagatcggcg agatggcggc ggcgcggtgc
      841 ggaccgggac cgtcccaggg acggtcgccg ccgagcacgc tctcgaaggt cggcatgtcg
      901 ctggtcatgg tcatcatgcc ccagtcgctg accccggcgc ccatcacccc ggccttgaag
      961 cggctcgttt gcgtcaccgc ccaggcggtc atgaagccgc cctgactcca gccgccgatg
     1021 ccgagccgcg cggggtcggc gatgccgcgc tcgatggccg cgtcgacgcc cgagaggacg
     1081 tcggggaagt cgccctcgcc gacgttgccg cgggcgtgcg cggcgaaggc attcccgtgc
     1141 cccatccccc cgcggtagtt cggcagcagc accgcgtagc cgtgcgccgc cagccactgc
     1201 gcccagttga gcgggcgcag gttccagccg ttggaggagc gcccgtaggg accgccgtgc
     1261 accagcacca ccagcggtag cggaccatcc ggcgcctgcg gtgggcggat cagcacgccg
     1321 tcgagcggca ggccgtcggg cgcggtccag tggaagggtt cttgccggcc gaactcgaac
     1381 tcgccgaggg ccgcgtggtg cgcgctgagc ggctggagct ggcccggcgt gccgccgtac
     1441 agctccggcg gccggtcccc ggcgctctcc accacggcca gcttcaccgc gccatccacc
     1501 acgcgcacgt cgaatgaact gatgtcgccg tccctgggcg cgtagagcgg ctcccgcgcg
     1561 ccgctctcgg tatcgaccca ctccagtcgc gtgctcagcc cctcggcgac gctgatcacc
     1621 acccgggccg agcccggtac tgccttcagc ccggtgacgc agcattcctc atcggcgctg
     1681 gggccgagca cgcgcggcct gccgcccgtc gcgtcgaccg cgtagagggt gaaggagccc
     1741 tgcggctcgg ggtcgtgcga gccgacgaag aggagttttg cgccgtcgtc gctccaggtc
     1801 agttggtgcg ccgaccagcc gttggcctca tccaccaccg tggtctgctg ctcggggtgc
     1861 tcgaccgcga tggtcgcgat ccgcgcccgc aggtgcgact cgatctccgg cgtgcgccag
     1921 gcactgaagg cgatgcgtgc gccgtccggc gaccaggcca gctcggccag gtgcgcctcc
     1981 ccggtgggaa gggtggtgac cgcgccgctc gccagatcga gcagatgcag gcgcgccagc
     2041 ggccagcgct cgccgtacac ctcggcgtca tcgcgctcct tctcgcgccg ctcgtcctct
     2101 tccgtcggct catccggcgc caggaaggcg atgcgccggc cgtcgggcga ccaggcgaag
     2161 ctggcgatgc ctcgcttgcg ctcgaccagc ggctcgggct cgccgccgct cagcggcatg
     2221 cggtagagcg cggcgatgcc cggcttgacc cgctcggaga ggaaggccag gctctgccca
     2281 tcgggcgacc agcgcggccg ctcgtcgcgc ccgttcccct gggtgatctg gcgcgccggc
     2341 gtgccgccgt cgagcggcgc cagccagatg gcgccctcgg cgtccttctc gcggccgtag
     2401 ggcgtggcga cataggccac ccgccgccca cccggcgcga tgcgcggatc ctccgggaac
     2461 gagagcgaga cgatcagctc ggccagatct tcgcgcgccg gcatgtgggc gaccccttcc
     2521 gtgtgcagcg ggagataggc gaatgtcctg aggcacagga tagcatcatg cctctagcgc
     2581 ggacgcaccg catcaggtag cctgcggcgc agcgcgttcg cgacggttcg accgggggga
     2641 gatcatgcgg cggtccgtgg cgctgctcgc cgtcatcatc gcgctgcagg tggggctggc
     2701 cggctggctg ctggccgacc accgcagcgg cgccgccggg gcgcggctgg cgcccgatct
     2761 gaccgtggtg acgcccagcc actggcggcc ggccacgctg gggcgccagc tcacggtgat
     2821 gacctacaac ctgcaggagg gcttctcgcg cgacgaccgc ttcgatctgg agcgccaggc
     2881 ggcggcgatc gcggccgagc gccccgacgt ggtggtggta caggaggtca gtcgcggctg
     2941 gctggtggcg gccggcgccg atgaagtcct ctggctcagc cagcggctag ggatgcaggc
     3001 catcttcggc gccaactccg acgacggcct ctggggcaac gccatcctca gccgcgcgcc
     3061 gctcacggac gccgcgcgcg agcaatacac cagcacccaa aacctcaagc gctccgtggt
     3121 ctcggcgcgc gtcgagaccg ccgccggccc gctctggatc tacggcaccc acctcgacaa
     3181 cccggtcggc gcgacccagg tacggctgga ccag
//
LOCUS       DASUBI010000246         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_785848, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000246 DASUBI010000000
VERSION     DASUBI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785848"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFU72_04720"
     CDS             complement(<1..1116)
                     /locus_tag="VFU72_04720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU5422821.1"
                     /translation="MAVVGLLGSGSDALAANEPHLTAQYAVVVDADTGKVLFDKNMDA
                     PAAPASLTKLFTAAVALSSASLDQELTVDSYDLVGESSMGLSAGQTVTLRTALYGMLL
                     PSGNDAAMTVAQNLGALPGDTPQQSIDRFMARVNATAARLGLQHTHLVTPHGLDAPGH
                     QTTARDIAAMTMFALRNPDFRTIIGSDTYKGEGFDLLQANRLLGSYPGLIGGKTGITD
                     NAGYCLMEAAERDGHTIIAVVLKSTADAWYTDASSLLDYGFGMLAGGTLPSDLPTISL
                     APVAPQAPVVQQPATGAVASTPAGMTVQRVSEHEVVVGHRDLVNVTHLSWHWPLAAVL
                     AMILATALALNYHLLIALAGLGWQHRPRLEPRRARGGR"
     gene            1424..2740
                     /locus_tag="VFU72_04725"
     CDS             1424..2740
                     /locus_tag="VFU72_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU5422822.1"
                     /translation="MTSDFPGRGKVAVLRTQPETVLDDIGQVMTLAGYQEALPNDLDT
                     ILKINVSWQTWYPACSTTPWQLEGVIRRLRADGYQRLIGAHNKTVVVDAYVGERNNKH
                     KYVVDKYGVENVHLYEPEIEWSIYEPKAEMLVLHDIFPEGIRIPRMFIGRNIIQLPTV
                     KTHVFTTITGAMKNAFGGLLNDRRHWTHAVIHETLVDLLTIQQEIHPGLFAVMDGTFA
                     GDGPGPRAMRVHEKDILLASADQVAIDAVSAKLQGFDPMSLDFIRLAHEKGLGVGDPR
                     DIEIVGADVSAEDWGFVREDTFASRGQHLIYHGPLKRFEKPLLRSPLVPWSYFASNFY
                     HNVYWYPFVGRPRVKAALDTKWGRLFQSYGDGRVVLPGPQPGATAVAGALAAAALGAS
                     ALVARRAFTGRASGPALPQHNRPCGSARPGQPEPPTKSAESPRGWA"
     gene            complement(2782..3396)
                     /locus_tag="VFU72_04730"
     CDS             complement(2782..3396)
                     /locus_tag="VFU72_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422823.1"
                     /translation="MLRVAEALNSKGEGVMAETVAAETTSRLHAIDELVLKVADDLSE
                     DELNWRPSQEAPSIAFHLWHIARWSDRNQVTIPRMAVDLANRFATQERWLSAGVARDW
                     GLDPARLGRGQSGIGMSDSDADQFRPPTKEALVDYLRQSLADVEQVYAAIDDRLAVQP
                     STVPEDNGATLGGAMLSHLTHVCRHLGMIEALRGVHGQRGSATA"
     gene            3518..4600
                     /locus_tag="VFU72_04735"
     CDS             3518..4600
                     /locus_tag="VFU72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEU5422824.1"
                     /translation="MRAAVCYEFGQPLVIDEVELDEPGRGEVRVKIAAVAICHSDVHL
                     IRGEWGGELPVVAGHEAAGIVDAVGEGVDSVQPGDHVIVSLLRSCGHCFYCTTGSPYV
                     CESQFPLRTETRLHTPAGEPILQGINCGAFAEYAVVHQSQLVLVDDELPLDSLALLAC
                     GVITGAGAVINTGGVRPGESVAVIGTGGVGLNAVQGAALAGANPIIALDLLDSKLEAA
                     RAFGATHALNAADPELPARVRALTGGRGPDYAVVTVGSVAAIEQALRLVRREGTVVVA
                     GMPHVDASFALPVYRFVGSGRRIVGSVMGSTRLHVDVPRFVELYQQGRLKLDQLISNR
                     YPLESINAAIESMESGQALRNVIVFD"
BASE COUNT          765 a         1574 c         1572 g          758 t
ORIGIN      
        1 tcgaccgccg cgagctcgcc gcggctccag ccgggggcgg tgctgccagc ccaggccggc
       61 cagcgcgatc aacaggtgat agttgagcgc cagcgccgtc gctaggatca tggccagcac
      121 cgccgccagc ggccagtgcc aggagaggtg cgtcacgttc accaggtcac ggtggccgac
      181 gaccacctca tgctcgctga cgcgctgcac cgtcatcccg gccggcgtcg acgccaccgc
      241 gccggtggcc ggctgctgga cgaccggcgc ctgtggcgcg accggcgcga gcgagatggt
      301 gggcagatct gacgggagcg tgccaccggc aagcatgccg aaaccgtagt cgagcaggct
      361 cgacgcgtcg gtgtaccagg cgtcggcggt gctcttgagc acgaccgcga tgatcgtatg
      421 cccgtctcgc tcggccgcct ccatcaaaca gtagccggcg ttgtcggtga taccggtctt
      481 accgccgatc aggccgggat agcttccgag cagtcgattg gcctggagca gatcgaagcc
      541 ctcgcctttg tacgtgtcgc tgccgatgat ggtgcgaaag tcggggttgc gcagagcgaa
      601 catcgtcatc gcggcgatgt cgcgcgccgt ggtctggtgg ccgggcgcgt ccagcccgtg
      661 cggcgtaacg aggtgcgtgt gctgcaggcc gagccgggcc gccgtggcgt tcacgcgcgc
      721 catgaagcgg tcgatcgact gctgcggcgt gtcaccgggc agcgcgccca ggttctgcgc
      781 caccgtcatg gcggcatcgt tgcccgaggg gagaagcatg ccgtagaggg ccgtgcgcag
      841 ggtaaccgtc tggccggcgc tgaggcccat cgaggactcc ccgacgagat cgtagctgtc
      901 gacggtcagc tcctgatcga gcgacgcgct gctcagggcg acggccgcgg tgaagagctt
      961 ggtcaggctg gccggcgcgg ccggcgcatc catatttttg tcgaagagaa ccttgccggt
     1021 gtcggcgtcg accacgacgg cgtactgcgc ggtgagatgt ggttcgttcg ccgccagcgc
     1081 gtccgagccg ctgccgagca agccaacgac cgccacgagc gaagccagaa gacacgcaca
     1141 gagccgtgcc tgtgagatgg cacgcgccac cgcagacccc caatcgggct tcccgaggtt
     1201 cccgcgcacg gcccccggct gtgtcagtcc ccccgggacc aacgcgcccc caccgtccgg
     1261 caagtcccca ttcgctgggc gggacaggtc gcgctatgac cgtgcgtcac gatgatagca
     1321 cgaacgaact gtacgtacaa gcgggtctga gtgggtttct gtcgtgaggc gtgctactat
     1381 cgagcctgcg taggcgggtt caggagagaa aagacgagga cgcatgacga gcgatttccc
     1441 gggacgtggc aaggtggcgg tgctccgaac ccagccggag acggtgctgg atgacatcgg
     1501 ccaggtgatg acgttggccg gttaccagga ggcgctgccc aacgatctgg atacgattct
     1561 caagatcaac gtctcctggc aaacctggta tcccgcctgc tcgactacgc cctggcagct
     1621 cgaaggggtg atccgccggc tgcgggcgga cggttaccag cgcctgatcg gcgcccacaa
     1681 caagacggtg gtcgtggatg cctacgtcgg tgagcgcaac aacaagcaca agtacgtggt
     1741 cgacaagtac ggcgtcgaga atgtccacct ctacgagccg gagatcgagt ggagcatcta
     1801 cgagccaaag gccgagatgc tggtgctcca cgatatcttc cccgagggca tccgtatccc
     1861 gcgcatgttc atcgggcgca acatcattca gctccccacg gtcaagaccc acgtcttcac
     1921 caccatcacc ggcgccatga agaacgcctt cggcggctta ctcaacgacc gccgtcactg
     1981 gacgcatgcg gtcatccacg agaccctggt cgatctcttg accattcagc aggagattca
     2041 ccccggcctc ttcgcggtga tggatggcac cttcgccggc gatggacccg gcccgcgcgc
     2101 catgcgtgtg cacgagaagg acatcctgct ggccagcgcc gaccaggtcg cgatcgacgc
     2161 cgtcagcgcc aagctgcaag gcttcgaccc catgtcactc gacttcattc gcctggcgca
     2221 cgagaaggga ctcggcgtcg gcgacccgag ggacatcgag atcgtggggg cggacgtgtc
     2281 ggccgaggac tggggcttcg tgcgcgagga caccttcgcc agccgcggcc agcacctgat
     2341 ctaccacggc ccgctcaagc gcttcgagaa gccgctgctg cgctcgccgc tggtgccctg
     2401 gtcctacttc gcctcgaact tctaccacaa cgtctattgg taccccttcg tcggacgccc
     2461 gcgggtcaag gcggcgctgg ataccaagtg gggccgcctc ttccagagct acggcgacgg
     2521 ccgcgtcgtc ctccccggcc cgcagcccgg cgcgaccgcc gtggccggcg ctctggccgc
     2581 tgccgcactc ggagcgtcgg cactcgttgc ccggcgcgcc ttcactggcc gagcctccgg
     2641 tccggcactg ccccagcaca atcgaccttg cggcagcgca cgaccaggac agccggaacc
     2701 tccgaccaaa tcggccgaat ctccgcgagg ctgggcgtag cctccggctc gccactaacg
     2761 ttgattcgcc gtcgcacacg actacgcggt ggcgctgccg cgctgaccgt gaactccacg
     2821 aagcgcttcg atcatgccca ggtgacggca gacgtgcgtc aggtggctca gcatggcgcc
     2881 gccaagggtc gcgccgttgt cctcgggcac agtggaaggc tggaccgcca ggcgatcgtc
     2941 gatcgcggcg tagacctgct cgacatccgc cagcgattgg cgcaggtaat cgaccagcgc
     3001 ctcctttgtc ggagggcgaa actgatcggc gtcgctgtcc gacatgccga tccccgattg
     3061 gccgcgtccc agccgcgccg ggtcgaggcc ccagtcgcgc gcgacacccg cggacagcca
     3121 gcgctcttgt gtggcgaatc ggttcgccag atcgacggcc atccgcggga tcgtgacctg
     3181 gttgcggtcg gaccaacgcg cgatgtgcca gaggtgaaac gcgatcgacg gcgcttcctg
     3241 gctcggacgc cagttcagct catcctcgga aaggtcgtcc gccactttca aaaccagttc
     3301 gtcgatcgcg tgcaaccggc tcgtggtctc cgcggcaacg gtctccgcca tgacaccttc
     3361 ccccttcgaa ttcaaggctt cagccacgcg cagcatagca tccaccagtt cagtccggac
     3421 cgatcgcgcc aactacgtgc ccgattgacc gcacctctcc actgctctag gctgggctca
     3481 tcggcgtggg agcatccggc acacgaggag ggcgaacatg agagccgccg tctgctacga
     3541 gttcgggcaa ccgctggtga tcgacgaggt cgaactggac gagccggggc gcggtgaagt
     3601 gcgcgtcaag atcgcggcgg tggcgatctg ccacagcgac gtgcacttga tccgcggcga
     3661 gtggggcggc gagctgccgg tcgtggccgg gcacgaggcc gcgggcatcg tggacgcggt
     3721 cggggaaggg gtggactcgg tccagcccgg cgaccacgtc atcgtctcgc tgctgcgctc
     3781 ctgcggccac tgcttctact gcaccaccgg ttcgccctac gtctgtgaga gccagttccc
     3841 gctgcggacc gagacgcgcc tgcacacgcc agccggcgag ccgattctgc aggggatcaa
     3901 ctgcggcgcc ttcgccgagt acgcggtcgt tcatcagtcg cagttggtgc tggtggacga
     3961 cgaattgccg cttgacagcc tggcgctgct cgcctgcggc gtcatcaccg gagcgggggc
     4021 ggtcatcaat acgggcgggg tgcgccccgg ggagagcgtg gccgtgatcg gcaccggcgg
     4081 cgtagggttg aacgcggtcc agggtgcggc gctggccggg gccaacccga tcatcgccct
     4141 cgatctcctc gacagtaagt tagaggcggc tcgcgccttc ggcgccaccc acgcgctcaa
     4201 cgccgccgat cccgagctgc cggcgcgagt gcgcgcgctg accggcgggc gcggcccgga
     4261 ctacgcggtg gtgacggtcg gcagcgtggc cgccatcgag caggcgctac ggctggtgcg
     4321 ccgcgagggc acggtggtgg tggccgggat gccgcacgtc gacgccagct tcgccttgcc
     4381 ggtctaccgc ttcgtcggct cgggccgtcg catcgtcggc agcgtgatgg gttcgacccg
     4441 cctgcacgtg gatgtgccgc gcttcgtcga gctctaccag cagggccggc tcaagctcga
     4501 ccagttgatc agcaaccgtt accccttgga gagcatcaac gcggcgatcg agtcgatgga
     4561 atccggtcaa gcgctgcgca acgtcatcgt gtttgactaa ccggcacggg ctctttcggc
     4621 tccggatcct tggcgtagat gccgtggtac ttgggcggca gcagcttga
//
LOCUS       DASUBI010000247        10540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1218640, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000247 DASUBI010000000
VERSION     DASUBI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218640"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..2041)
                     /locus_tag="VFU72_04740"
     CDS             complement(464..2041)
                     /locus_tag="VFU72_04740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5422825.1"
                     /translation="MHRTLLSSRHWRWLLALLASGVLLLGAAGAGLADDDEGQVPPPF
                     GFSGFAAQGKGQPFVTIPARGAALYPSAKPVPSGGLSVGSNVNIIGRSDVVQQATIDG
                     VDVQTCNPGMQTAQNETPIAVNPANADNLVAGANDYRTYESSENRYDSGGGFYRSTNG
                     GTSWIAGFLPGLVRANGGPYESAGDPAIAAGPDDVFWYANLVFNRSDAASGVAVSRST
                     DGGATWTTSYVLQTSAAAGATLFNDKEWIAADPANPNVAYVTWTQFHSGPTGHTTSSI
                     IVLSQTSDGGAHWSAPVAISPFLYSQGSVGQVDDAGQVHIVYEAFSHGKNVIAHSVDD
                     PASGAVQTEIASVENFVPSPLPGMTFRTNSFPGFALDGNTLHVVWANWNGSDADVVYT
                     QKTLGGAWSAPVTLGTVAGDQFFPWVGAMNGKVYVSWMNSAGANDTYTEQATGSADGG
                     LTWTAPVTVSSASSTVTNGNEFGFPDCGPVFIGDYTGIAVDANGAAHPMWTDIRIGND
                     GRRTADQDPYTAVLTLK"
     gene            complement(2207..3301)
                     /locus_tag="VFU72_04745"
     CDS             complement(2207..3301)
                     /locus_tag="VFU72_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5422826.1"
                     /translation="MDAADIARHRLVNLRITGHAGTTPAEVVAALGAIQAQDFVAGKW
                     AIGLRLPGSTEADIERALDDGAIVRTWPMRGTLHFVAAADVRWLLRLLAPRVIAGSAG
                     RLRALEIDDAVLARSRTVMTRALEGGQRLTRNDLYRRLDEAGISTAGQRGYHLLWLAA
                     HEGLICFGPLAGKQQAVVLVDEWLPGGAEAQRAREEALARLAGRYVSSHGPATLADFA
                     RWGGLTVSDARLGLHGAAGTLARETIDGQDFWLPRDAPASPAASPRVDLLPSFDEYLV
                     GYKERGAVLDAAHSQRVIPGSNGMFLPALVVDGRVVGTWKRVPVKGGVRVVATPFAPL
                     DAAAVRGFAAAASRYGAYLGLPAEAVMLDT"
     gene            3429..4652
                     /locus_tag="VFU72_04750"
     CDS             3429..4652
                     /locus_tag="VFU72_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422827.1"
                     /translation="MAKSEAAHAQRQPALSVVRNRPLMTLFFGHMTIDLYAGLLPILY
                     PVLSQRFTLDLDTVGLLTLAYSGVGSVSQPLFGWLADRYGTRLTGFALAWTACTFAAI
                     AFAPTFPIVLLLAGAAGLGSGAFHPFGALNANAVIPERQRNSAMSIYASGGTVGFAVG
                     PLIGVGLLAAFGMHGIAAMILPGALIAAWLLYEMRTIAVKSRRHGGQGFGAVAPIPLI
                     PVAAVILVMMARSWTMSSIQAYVPIWYEQLGYGKSFYGSLATAVTLASAAGTLGLGSV
                     ADRHGRRALIAGSLVLTIPSLLLFAQFTGPIAFVTGPLVGIIAASSAPLLLVMAQQLM
                     RGRAGIASGLILGLGFVTGAIGVPITGAVADSVGIQMAMRLQVIVVLIAIPLALLLPS
                     ERRFRELDEAGRQPA"
     gene            complement(5128..5931)
                     /locus_tag="VFU72_04755"
     CDS             complement(5128..5931)
                     /locus_tag="VFU72_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5422828.1"
                     /translation="MRMGMASAPRIARHPTAPAPASRLPHASREPRAAALDRLYTSVL
                     GGVGRTDRRSSLGPAWSSGPAWCLPLVVRRGIMPTRAEILDTLAASQARVIAFFHGLR
                     PHDLERPVTASGVPGAAPWRARDHLAHLVQSERNIQHLLRRTLAGDTRDVLLRLQYPA
                     GMSLPGTLGDWDALTPREQEQLELAVARVNQIALNDHQDDTLEQLAAAYLAARQDTLG
                     LLHQFTDEQLAAAVPTVVSERAAGDLFAGLAAHATGHITLIEDGWLQGG"
     gene            5924..6049
                     /locus_tag="VFU72_04760"
     CDS             5924..6049
                     /locus_tag="VFU72_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422829.1"
                     /translation="MRMLERDPWLVGCLLVLAVLIVAVVGTVVFLLVVRTSGVML"
     gene            6549..8510
                     /locus_tag="VFU72_04765"
     CDS             6549..8510
                     /locus_tag="VFU72_04765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5422830.1"
                     /translation="MQQQISPAAFVYDLQQPGDPQLSPDGARLVYTLTRVDPESKKTT
                     SQLWLCDRDGGNARQLTRGGVGEKHSGARWSPDGTAIAFVSDRVEAPAKNGIFVLPVA
                     QPGEARRLTSHNQALGELAWSPDGATLAYTTTFDPENPSEEPPKEGEPAKVRVTRRLD
                     YKMDGRGWLGDVRKQVWTVDVASGERTMRTQEPVDHSQPLWSPDGRTLAVRKQAPDGW
                     TSSIALLDLASGEQRRAGDVVGTHPSAAWSPDGSRLLITGDPEHSYQPDLYLVDVASN
                     QTRRLSDDLPILPIDGMGETALAWLDARHVLINAAAHARHGLYRLDVETEELICLDNQ
                     EASAVGLSLDAGGRFVAQARTSFESPGEITVHDLREGRTTPATGLNGALAAAAGPAGW
                     ERFELERGGFTIESWLLKPADYDPAHRYPVVLMIHGGPNGFFGYRFLANQQLLANHGY
                     LVVLPNPRGSTSYGREFAVQVLGDRGGEDYHDLMAVADQVAARPDVDPERIGIYGYSY
                     GGYMTAWILGQTDRFKACVCGAPSVDLTAQFGTSDIGWHYDVTQYRAQPHERPEWYRD
                     HSPLTYAHRTSTPTLLVSGESDLRCPTSQAEELFVALKKGGCEVEFVRYPGVGHGFAS
                     AGPSQYREDFLARTLAWFDRYLGGGSGQS"
     gene            complement(8594..9487)
                     /locus_tag="VFU72_04770"
     CDS             complement(8594..9487)
                     /locus_tag="VFU72_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5422831.1"
                     /translation="MDLSWQLAVAGALVGLCVGLTGMGGGALMTPILVLFFGVDPTTA
                     VGSDLLVSVTMKPVGAAVHQRSGMVRWELVRWIVPAGVPAGFAGAFVVHLLGNGALLQ
                     HRVKLAIGAALLAAVAGMIARVLLNRRGRKRAAAIRSSEPLVVRPVPTALIGLVGGFI
                     VGMTSVGSGSLIIVMLMLCYPQLRAAELVGTDLVQAVPLVGAAALGHLLAGDVRLALT
                     GSLLLGALPAIYLGAKLSTNVPSAVLKWILGVLLLASGLQLWGLSTALVCVISLTFAA
                     VVGLYRFGGSRYSTLGGRPTS"
     gene            complement(9749..>10540)
                     /locus_tag="VFU72_04775"
     CDS             complement(9749..>10540)
                     /locus_tag="VFU72_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5422832.1"
                     /translation="ELYAHQAAELVETSRLYREADRTVHDREEFLSTAAHELKTPLTT
                     VKGYSQLLWQQVQRDPLDQARLRLLVEHLGQQVGRMELLVADLLDISRIQQGHLVLRP
                     EEVDLVALAREVYERFVDAPERRLEHRLTFEAPEDLRGHWDPVRLDQILTNLLTNALK
                     YSPSGGPVWLRIARDEDEAVVEVADRGVGMSREERARIFQPFSRAESASRAISGVGLG
                     LYISAQIVRRHGGSIGVESEPGVGSIFTVRLPLGEQLRPPEGPGA"
BASE COUNT         1616 a         3789 c         3584 g         1551 t
ORIGIN      
        1 caacgcgctg cacaccgtcg tcgccgcgcg caaccacgac gcctggcaga tcgcgctctt
       61 ccagaacacc ccggcccagt accacggctg gcccgaattg acggagcagc tgaccgcgga
      121 gctacgcgag ctgctgtagg cgccgggtgg ccatcgagaa gcccacccgc cgcgtcaaca
      181 cggatccctc gactccgctc gggatgacag ggggaagagg acgcgtgcga cgagggtcag
      241 agccatcggc acaagaaggc ccaccgtgct cttgtagggg cagacctccg tgtctgccct
      301 cgctgcccta ccgccggaca ttctcggcag acgccgacgt tgctcctgtc accccttgcc
      361 gaggcgtcgg ggcaagaggg cagacacgga ggtctgcccc taccgtgaaa acggcgcggg
      421 gccgggagga cgcgccgtcc ccccggcctg gccgcctcct tcgttatttc agcgtcagca
      481 ccgccgtgta cgggtcctga tcggcggttc ggcgcccgtc gttgccgatg cggatgtcgg
      541 tccacatcgg gtgcgccgcg ccgttcgcat cgaccgcgat gccggtgtag tcaccgatga
      601 agaccgggcc gcagtcgggg aagccgaact cgttgccgtt cgtcaccgtc gagctggcgc
      661 tggagacggt caccggcgcg gtccaggtca ggccgccgtc ggccgagccg gtcgcctgct
      721 cggtgtaggt gtcgttcgcg cccgcgctgt tcatccagct cacgtagacc ttgccgttca
      781 tcgccccgac ccaggggaag aactggtcgc cggcgaccgt accgagcgtg actggcgcgc
      841 tccaggcgcc gcccagcgtc ttctgggtgt agacgacatc ggcatcgctg ccgttccagt
      901 tggcccagac gacgtgcagc gtgttgccgt cgagcgcgaa gccggggaag ctattggtgc
      961 gaaacgtcat gcccggcagc gggctgggga cgaagttctc cactgacgcg atctccgtct
     1021 gcacggcccc gctcgccgga tcgtcgacgc tgtgggcgat cacgttcttg ccgtgggaga
     1081 aggcttcgta gacgatgtgg acctggcccg cgtcgtccac ctgccccacc gagccctggc
     1141 tatagaggaa gggcgagatg gcgaccggag ccgaccagtg cgcgccgccg tcgctggtct
     1201 gcgagagcac gatgatcgaa ctggtggtgt ggccggtggg ccccgaatgg aactgcgtcc
     1261 aggtcacgta ggccacgttc ggattggccg gatcggcggc gatccactct ttgtcgttga
     1321 agagggtcgc acccgctgcc gccgaggtct ggagaacgta gctcgtcgtc caggtggcgc
     1381 cgccgtcggt cgagcggctc accgccacgc cgctggcggc gtcgctgcgg ttgaagacca
     1441 ggttggcata ccagaagacg tcgtccggcc cggcggcgat ggccggatcg ccggccgact
     1501 cataggggcc gccgttggcg cgcaccagac cgggcaggaa gccggctatc caggaggtcc
     1561 cgccgttcgt cgaacggtag aagccgcccc cggagtcgta gcgattttcg ctcgactcgt
     1621 aggtccgata gtcgttggcg ccagccacga gattgtcggc attggcgggg ttgaccgcga
     1681 tcggcgtctc gttctgggcc gtctgcatgc cggggttgca ggtctgcacg tcgaccccgt
     1741 cgatcgtcgc ctgctgcacg acatcacttc tgccgatgat gttgacgttg ctgcccacgc
     1801 tcagcccacc ggaggggacc ggcttggcgg acgggtacag ggcggcgccg cgcgccggta
     1861 tggtcacgaa cggctgcccc ttgccctgcg cggcgaagcc ggagaatccg aacggcgggg
     1921 gaacctgccc ctcgtcgtca tcggccagcc cggcgccggc cgcgccgagc agcaagacgc
     1981 cgctcgccag cagcgcgagt agccaacgcc agtgccgcga actcagaagc gtgcgatgca
     2041 tgcatggctc ccttcccgat cgcccacact ttcatcttgc cgcgaagtag agccactatg
     2101 cgcccggaca ccacttctgt caagctaggc cggtggggtt ctcggatgtc gggcaagcgc
     2161 gtgcgtgggt ccgcgcggag gtgcaatcgg ggaggatcgt gattactcag gtatcgagca
     2221 tgaccgcctc ggccggtagg ccgaggtacg cgccgtagcg gctcgccgcc gccgcgaagc
     2281 cgcgcacggc ggcggcgtcc agcggagcga agggcgtggc gaccacgcgc acgccgcctt
     2341 tcaccgggac gcgcttccag gtaccgacca cgcggccgtc gaccaccagt gccggcagga
     2401 acatcccatt gctgccgggg atgacccgct ggctgtgcgc tgcgtcgagc acggcgccgc
     2461 gttccttgta gcccaccagg tactcgtcga aactgggcag gagatcgaca cgcggtgagg
     2521 ctgcgggtga agccggcgca tcacgtggca gccagaagtc ctggccgtcg atcgtctcgc
     2581 gcgccagcgt cccggccgcg ccgtgaagtc ccagccgggc atcgctcacg gtcaggccgc
     2641 cccagcgggc gaagtcggcc agtgtcgccg ggccgtggct gctgacgtag cgcccggcca
     2701 gcctggccag ggcctcctcg cgcgcccgct gcgcttcggc gccgcccggc agccactcgt
     2761 cgacgaggac gacggcctgc tgcttgccgg ccagcggacc gaagcagatc agcccctcat
     2821 gcgccgcgag ccagaggagg tggtagccgc gctggccggc agtcgagatg cccgcctcgt
     2881 cgagccgccg gtacaggtcg ttccgcgtca ggcgctgccc gccctccagc gcccgagtca
     2941 tgaccgtccg gctgcgggcc agaaccgcgt cgtcgatctc cagcgcgcgc aggcgtcccg
     3001 cgctgccggc gatgacgcgc ggcgccagga gccgcagcag ccagcgcacg tcggccgcgg
     3061 ccacgaagtg gagcgtgccg cgcatcggcc aggtgcggac gatggcgccg tcgtccaggg
     3121 cacgctcgat gtcggcctcg gtcgaacctg gcaggcgcaa cccgatggcc cacttgccgg
     3181 cgacgaagtc ctgcgcctgg atggcgccga gcgcggccac gacctcggcc ggcgtcgtgc
     3241 ccgcatggcc ggtgatccgc aggttgacga gccggtgccg cgcgatgtcc gctgcgtcca
     3301 tagtcgccca gtatagtcac gcggagctgc cgtttcatct tgacgctgac cgggcgctcg
     3361 cgtagggttt tcggccagca ggagcatgca gaagcgcgcg ccagggacga cgatcggagc
     3421 agaccgagtt ggccaagagc gaagccgcgc acgcccaacg ccaaccggcg ctcagcgtgg
     3481 tgcgcaaccg tccgctgatg acgctcttct tcgggcatat gacgattgac ctctacgccg
     3541 ggctgctgcc gatcctctac ccggtcctct cgcagcgctt caccctcgat ctggacaccg
     3601 tcggactgct gacgctggcc tatagcggcg tcggctcggt ctcgcagccg ctcttcggct
     3661 ggctggccga ccgctacggc acgcgcctga ccggcttcgc gctggcctgg accgcgtgca
     3721 ccttcgcggc catcgccttc gcgccgacct tccccatcgt gctgctcctg gccggggcag
     3781 cggggctcgg gtccggcgcc ttccacccct tcggcgcgct gaacgccaac gccgtcatcc
     3841 ccgagcggca gcgcaactcg gccatgtcga tctatgcctc gggcggcacc gtcggcttcg
     3901 ccgtcgggcc gctgatcggc gtggggctct tggccgcctt cggcatgcac ggcatcgcgg
     3961 ccatgatcct gcccggcgcg ctgatcgccg cctggctcct ctacgagatg cgcaccatcg
     4021 cggtcaagtc gcgtcggcac gggggacagg gcttcggcgc ggtggcgccg atcccgctga
     4081 tcccggtcgc cgcggtcatc ctggtgatga tggcgcgctc ctggacgatg tcgagcatcc
     4141 aggcctacgt gccgatctgg tacgagcagc tcggctacgg caagtcgttc tatggctcgc
     4201 tggcgaccgc ggtgacgctg gccagcgccg ccggcacgct cggcctgggc agcgtggccg
     4261 accgccacgg gcgacgcgcg ctgatcgccg gctcgctggt gctgaccatc cccagcctgc
     4321 tcctcttcgc ccagttcacc gggccgatcg ccttcgtcac cggcccgctg gtcgggatca
     4381 tcgccgcctc ctcggcgccg ctgctgctgg tgatggcgca gcagctgatg cgcggacggg
     4441 ccggcatcgc ctccgggctg atcctcggcc tcggcttcgt caccggcgcc atcggcgtgc
     4501 cgatcaccgg cgcggtggcc gactcggtcg gcatccagat ggcgatgcgc ctccaggtca
     4561 tcgtggtgct gatcgccatc ccgctggcgc tcctcctgcc cagcgagcgc cgcttccgcg
     4621 agctggacga ggcgggaagg cagccagcct agcctgagcg gcgcgcgggc gcggccccct
     4681 gctgtcgtcg gcgcggcggc cgtgcgcacg agtgacaagc cagcgggcag ctcggatgga
     4741 gcgcgcgacc gtctcactgc tctggcaaac gtcttgagca tcgggctcga agacgcgcgc
     4801 gtatttgaag cacgcgcgtg ccaattcacg ctgggcgaac cggatcaacc gctctgcctg
     4861 atcccgagcg catcgcctat cgtgaggccg gacccgttgt cgccgcttcc cgcctcctga
     4921 aggacacgct cacgggcccc gacaccagat gcatccccac cacgggccac acgccttccg
     4981 atccctctct ttgagacaga agggaaccaa ccggtgtgga tccgtctccg catccgcacg
     5041 acgcgatcgc ccagcaaagc gcacggcaac agcagtccga ttcccaccgg ggcatgacgg
     5101 cgccgaccgg cgaagaccgc aagccttcta cccgccctga agccatccgt cctcgatcaa
     5161 ggtgatgtgc ccggtcgcat gcgccgcgag cccggcaaac aagtcgccgg cggcccgctc
     5221 actgacgacc gtcggcaccg cagcggccaa ctgctcatcg gtgaactggt gcaggaggcc
     5281 caaggtgtcc tgccgcgccg ccaggtacgc cgcggccaac tgctccagcg tgtcgtcctg
     5341 gtggtcgttc agggcgattt gattgacgcg ggcgacggcc agctccagct gctcctgctc
     5401 cctcggggtc agcgcatccc aatcgcccag cgtgcccggc agcgacatgc ccgccgggta
     5461 ttgcaggcgc aagagcacgt ctcgcgtgtc gccggcgagg gtacggcgca gcaagtgctg
     5521 gatgttgcgc tcgctttgca ccaggtgcgc cagatgatcc ctggcgcgcc atggagctgc
     5581 tccgggaacg ccgctcgccg tgaccgggcg ctccaggtcg tgcggtctca gtccgtgaaa
     5641 gaacgctatg acccgcgcct gcgacgccgc cagggtgtcc agaatctccg cgcgcgttgg
     5701 catgattccc ctccgaacaa ccagtgggag gcaccaggcg ggtccagacg accacgccgg
     5761 gccgagagac gagcggcgat cagtgcggcc cacgcctccg agaacagagg tatatagcct
     5821 atccaacgct gccgcacgtg gttcacgaga cgcgtgcggc agccggcttg ctggtgccgg
     5881 cgccgtgggg tgccgtgcta tcctcggcgc ggaggccatg cccatgcgca tgctagagcg
     5941 cgatccctgg ctcgtcggct gcctgctggt gctcgcagta ctgatcgtgg cggtcgtcgg
     6001 tacggtcgtc ttcctgctgg tggtgcgcac cagcggcgtc atgctctaga gaggccggcg
     6061 agcgcgtagg gcaggctcgc cggccaaagg tgagggggaa aggtcgggga atcacgggtt
     6121 agcccggcag cacgcccagc tgccgctgca tgccggcgaa gtccgcctgg atccactgtt
     6181 cggcgccctg gccgccttcg ataaggagtg tgctcgtctg cgacatgacg tgctccaatc
     6241 gtcatcgccc ggtgacgggg catggtgcgt gatcgtgtgg cgcttctgct tggccgcgcg
     6301 gatgggagat ccttcggcgg cggcctcagg atgagagcgc aagaataatc cctggactgc
     6361 actcctctcg gtgtgccggt ccgatgcctc cattctcttc ccggccccgg ccagtcacca
     6421 gtccaatggc tgcgcattcc ctgctcagtt tcgcggcacg agcgtgctat gatcgacagc
     6481 ccgcgaggat tggctgccca gcgccggcct accgtcggcg acgccgggcg cgacaggagg
     6541 aagacccgat gcagcagcag atcagcccgg cggcgttcgt ctacgacctc cagcagcccg
     6601 gcgatcccca gctctcgccc gacggcgcac gcctcgtcta cacgctgaca cgcgtcgatc
     6661 ccgagagtaa gaagaccacc agccagctct ggctctgcga ccgtgacggc ggcaatgcgc
     6721 gccagttgac ccgtggcggg gtgggcgaga agcacagcgg cgcccgctgg tcgcccgacg
     6781 gcacggccat cgccttcgtc tccgaccgcg tcgaggcgcc cgccaagaac ggcatcttcg
     6841 tgctgccggt cgcgcagccc ggcgaggcgc gccggctcac cagccacaac caggcgctcg
     6901 gcgagctggc ctggtcgccc gacggcgcga ccctcgccta caccaccacc ttcgacccgg
     6961 agaacccgag cgaggagccg cccaaggagg gcgagccggc caaggtgcgt gtgacccggc
     7021 ggctcgacta caagatggac gggcgcggct ggctcgggga tgtgcgcaag caggtctgga
     7081 cggtcgacgt cgcctcgggc gagcgcacga tgcgcacgca ggagccggtc gaccacagcc
     7141 agccgctctg gtcgccggac ggccgcacgc tggccgtgcg caagcaggcg cccgacggct
     7201 ggaccagctc catcgcgctg ctcgacctcg cctccggcga gcagcgccgc gcgggcgacg
     7261 tggtcggcac ccacccctcg gcggcctggt cgccggatgg cagccggctg ctcatcaccg
     7321 gcgaccccga gcacagctac cagcccgatc tctacctggt cgacgtggca agcaatcaga
     7381 cccgccgcct gagcgacgac ctgccgatcc tgccgatcga cggcatgggc gagacggcgc
     7441 tggcctggct cgacgcgcgg cacgtgctga tcaatgccgc cgcgcacgcg cgccacggcc
     7501 tctaccggct cgacgtcgaa accgaggagc tgatctgcct ggacaaccag gaggccagcg
     7561 cggtcgggct gagtctcgac gcgggaggcc gcttcgtggc gcaggcgcgc accagcttcg
     7621 agtcgcccgg cgagatcacc gtccacgatc tgcgcgaggg ccgcaccacg ccggcgaccg
     7681 gcctgaacgg cgcgctggcc gcggccgctg gcccggccgg ctgggagcgc ttcgagctgg
     7741 agcgcggcgg cttcaccatc gagtcgtggc tgctcaagcc cgccgactac gaccccgcgc
     7801 accgctatcc agtcgtcctg atgatccacg gcgggccgaa cggcttcttc ggctaccgct
     7861 tcctggccaa ccagcagttg ctggccaacc acggctatct ggtcgtgctg cccaacccgc
     7921 gcggctcgac ctcctacggg cgtgagttcg cggtccaggt gctcggcgac cgcggcggcg
     7981 aggactacca cgacctgatg gcggtcgccg accaggtcgc cgcccggccc gacgtggacc
     8041 ccgagcgcat cggcatctac ggctacagct acggcggcta catgaccgcc tggatcctcg
     8101 gccagaccga ccgcttcaag gcctgcgtct gcggcgcgcc ctcggtcgac ctgaccgcgc
     8161 agttcggcac cagcgacatc ggctggcact acgacgtgac ccagtaccga gcccagccgc
     8221 acgagcggcc ggagtggtac cgcgaccact cgccgctgac ctacgcccac cgcaccagca
     8281 cgccgacgct gctggtcagc ggcgagagcg acctgcgctg ccccaccagc caggccgagg
     8341 agctgttcgt ggccctgaag aagggcggct gcgaggtcga gttcgtgcgc taccccggcg
     8401 tcggccacgg cttcgccagc gccggcccgt cccagtaccg cgaggacttc ctggcgcgca
     8461 cgctggcctg gttcgaccgc tacctcggcg ggggaagcgg gcagtcctga ttcatttctg
     8521 gctgaaacgc ggacggctcc gtccagcacc cacaccggac ggagccgccg gagccgccac
     8581 acgagcacgc aattcaggat gtcgggcggc cccccaatgt cgagtagcgc gaaccgccaa
     8641 agcggtacag gccaaccacg gcggcgaagg taagagagat gacacagacg agggctgtgg
     8701 agagtcccca gagttggagc ccggaagcca gcagcaggac gccgagaatc cacttgagca
     8761 ccgcgctcgg cacgttggtc gagagtttcg cgccaaggta gatcgctggg agcgcgccca
     8821 gcaggagcga gccggttagg gccagccgaa catcccccgc cagcagatgg ccgagcgcgg
     8881 cggcgccgac cagcggcacc gcttgcacca ggtcggtgcc caccagttcg gcggcgcgca
     8941 gttgcgggta gcagagcatc agcatcacga tgatcaggga gcccgagccg accgaggtca
     9001 tgccgacgat gaagccgccc acgagaccga tcagcgccgt cggcaccggc cgcaccacca
     9061 ggggctccga tgacctgatc gcggcggctc gcttgcgccc gcgccggttc agcagcaccc
     9121 gggcgatcat gcccgcaacc gccgccagga gggcggcgcc gatcgccagc ttgacccggt
     9181 gctgcagcag tgcgccgtta cccagcagat gcaccacaaa cgccccagcg aagccggccg
     9241 gcacacccgc cggaacgatc cagcggacca gctcccagcg caccatgccg gaccgctggt
     9301 gcaccgcggc gccgaccggc ttcatcgtga ccgagaccag caggtcgctg ccgacagcgg
     9361 tggtcggatc gaccccgaaa aagagcacca ggataggcgt catcagcgcc ccaccgccca
     9421 tgccggtcaa tccgacgcac aggccgacta acgcgccggc gacggcaagc tgccaagaca
     9481 ggtccatgcc tcaatcctcc atgcctggcg tcgttggccc gtgtcgatcg cggggggact
     9541 ggaacactat cggcggacga tctcagcgga gatgccgagg ccggcgttcg ccagcggacg
     9601 ctggtggagc gcatgctcgg aaacggcgcc cgtgtcgatt cactccagga aagccccgtc
     9661 tggtggagcc gtctccatca ggcgcgttgc attctggtga aggagcgcgg agcgtagcag
     9721 tcggcggtga tcgcccggcc cggcctggct aggcccccgg gccttccggc ggccggagct
     9781 gctctccgag cggcaggcgc acggtgaaga tgctgcccac acccggctcg ctctcgacgc
     9841 cgatgctgcc gccgtggcgg cggacgatct gggcgctgat gtagaggccc agcccgaccc
     9901 cactgatcgc ccggctggcc gactccgctc gcgagaacgg ctggaagatg cgcgcccgct
     9961 cctcgcggga catgccgacg ccgcgatcgg cgacctcgac gacggcttcg tcctcgtcgc
    10021 gcgcgatccg cagccagacc ggcccgccgc tcggcgagta cttcaacgcg ttggtgagca
    10081 ggttggtgag aatctggtcc agccgcaccg gatcccagtg gccgcgcaga tcctcgggcg
    10141 cctcgaacgt cagccggtgc tcgaggcgcc gctccggcgc gtccacgaag cgctcataca
    10201 cctcgcgcgc cagcgcgacg aggtcgacct cctcgggccg cagcaccaga tggccctgct
    10261 gaatgcgcga gatgtcgagc aggtcggcca ccagtagctc catgcgaccc acctgctgcc
    10321 ccagatgctc gaccagcagc cgcagccggg cttgatcgag cggatcacgc tgcacctgct
    10381 gccacaggag ctggctgtag cccttgaccg tggtgagcgg ggtcttcagc tcgtgggccg
    10441 ccgtcgagag gaactcttcg cggtcgtgca ccgtgcgatc ggcctcgcga tagagccggc
    10501 tggtctcgac cagctcggcc gcctggtggg cgtagagctc
//
LOCUS       DASUBI010000248         5123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_508881, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000248 DASUBI010000000
VERSION     DASUBI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508881"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFU72_04780"
     CDS             <1..702
                     /locus_tag="VFU72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEU5422833.1"
                     /translation="LIAAVLAATVLPLAPGAVVVNLATGALFVNAALAYVMVALVMAG
                     WGPDGVYAMIGGWRFLGQLVAYSMLIVMPLTAVVMRAQSLVVVTVVTAQAHLWNIVAQ
                     PLGFVLFWLAALAVCFLPPFDLPVAPDLAGGVWAEYTGVRLAVMRLARLLLVLVLALT
                     VTVFFLGGWLGPALPPWLWTALKTLAVAALFLTGGRFVPRLRDEQVLAWAWKYGIPLA
                     LVNIFWVGVTLLTVR"
     gene            741..1223
                     /locus_tag="VFU72_04785"
     CDS             741..1223
                     /locus_tag="VFU72_04785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5422834.1"
                     /translation="MIGFGALALWFGVAVFRTSSMVRSALALLFAMAALGGLFLALEA
                     EFLGVLQLMMMATEMAIMAIFMVMYMMDPGGLGQMEMTHQKRRALAAGTLGGLVSAAL
                     SLLTGWGPLTRAAPDPVAQVHDLGRELLERSMLIFETAGVTILVAMVAATAVAIGRRA
                     "
     gene            1220..1528
                     /gene="nuoK"
                     /locus_tag="VFU72_04790"
     CDS             1220..1528
                     /gene="nuoK"
                     /locus_tag="VFU72_04790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5422835.1"
                     /translation="MMTFSLTLLVGAGLFGIGLYGALSQTNLVMIMMGVELMLGAALV
                     NLVVFWRFLHPDVYSGQVFVLIVMTVMALEMTVGFGVATARFRAKGSVEMEEAAELQG
                     "
     gene            1727..3424
                     /locus_tag="VFU72_04795"
     CDS             1727..3424
                     /locus_tag="VFU72_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5422836.1"
                     /translation="MVDPRAASLSAVVAVVTLLVLVYAVGYMAGDQAQPRFVAGMTFF
                     AAAMQALVLAGDWLLFLTAWELIGFASYLLIGFWFTRPGVGVAATRAFVTTRAADLGL
                     YVGIFLLTTHAGTTAIAPTLHAQGSAARVAGLLLLLAAAGKAGQVPFQGWLQDAMVGP
                     TPVSALLHSAALVAAGAVLLIRASPLLDGPVLLVIGVVGGTTALVTGLMALAQGDLKR
                     LLASSTSSQLGFMFLAVGAGAPVAALVHLVAHAAMKSALFLGAGVFQHAREATTFDRL
                     AGVGRTHRATYVGFGLAGLALAGVPPLAGFWSKDAVVAATVASPYHWALAPLALLGTL
                     LTGGYVARALRLLWQGAGVQQPVRGLAWMGAGLAALTGLAAALGLAVHPLAHLLNATL
                     PENRVATLGALVLAVAGLALGATLPIARLLGPLRGWGERGFRLGGGFAGLVVHPALAL
                     ARQADRLDQAIHRGVFAVGHGGIAVARAARTLDQVDERLAGSVGRLALALAQAARWTD
                     EDAIDALIAGLVQRTRDLGARARRAQTGLVHQELLVAAGGAALLLVVLVAGLVLTGR"
     gene            3455..4924
                     /locus_tag="VFU72_04800"
     CDS             3455..4924
                     /locus_tag="VFU72_04800"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5422837.1"
                     /translation="MVPLVSLTLFLPLFGALLVAVWPGASARVCHSVGLGAAGLSLLG
                     AMGIWLRGVGTGFGQIEEVRWIPSLGAAYRVGVDGINLPLVVLTAILFLAALVFSTNI
                     TDRPRAYVALFLLLETSCLGVFLALDLVLFYVFWEVTLVAMYFIIAGWGHAGRQRAAL
                     TFFLYTLLGSLPLLLAILALYLGSRPRTFDVRALVAAPPLTGGAEMLTFIALLLAFAV
                     KSPLFPLHTWLPAAHVEAPTAGSVVLAGVLLKLGTYGFIRFALELTPGAFRTAAPVVA
                     GIAVFSALYGAFVALAQTDLKRLVAYTSVNHMGYVVLGVAAAAAATDRGARALALDGA
                     TLQMVSHGLVTGVLFLLVGTVQDRAKTRELGALSGLLRVIPVIGWGFILAAFASFGLP
                     GLAHFPAEFQIFLGAFQVWPWAAAIVVLGIAVTAGLYLRAIQIAFLGEAPGRWQAVPD
                     LNAREAWAILPLHMLTVAVGLGPGAVLDLIHRATQAVGG"
     gene            4921..>5123
                     /locus_tag="VFU72_04805"
     CDS             4921..>5123
                     /locus_tag="VFU72_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422838.1"
                     /translation="MTGAATLAMGHDLALLLPQLLVLVTAVGALVAEMLQRPRAALVI
                     TVAGLLVAAMLAATRLDQQVTVFL"
BASE COUNT          539 a         1765 c         1825 g          994 t
ORIGIN      
        1 ctgatcgccg ccgtgctggc cgccactgtc ctgccgctcg cgcccggcgc cgtggtggtg
       61 aacctggcga ccggagcgct cttcgtcaac gcggcgctcg cgtacgtgat ggtggcgctg
      121 gtgatggcgg gctgggggcc agacggtgtc tacgcgatga tcggcggctg gcgtttcctg
      181 gggcaactcg tcgcgtattc catgctgatt gtgatgccgc ttaccgccgt cgtcatgcgc
      241 gcgcagtcgc tcgtggtcgt caccgtcgtc acggcacagg cacacctgtg gaacatcgtg
      301 gcccagccgc ttggcttcgt cctcttctgg ctggcggccc tggccgtctg cttcttgccg
      361 ccgttcgacc tgccggtcgc ccccgatctg gcgggcggtg tctgggcgga atacaccggc
      421 gtgcggctgg cggtcatgcg cctggcgcgg ctcctgctcg tcctggtgct cgcgctcacg
      481 gtcaccgtct tcttcctggg tggctggctc gggccagcgc tgccgccgtg gctctggact
      541 gcgctgaaga cgctcgctgt cgcggcgttg tttctcacgg ggggccgctt cgtgccgcgt
      601 ctacgcgatg agcaggtact ggcctgggcc tggaagtatg ggattccgct ggcgctggtg
      661 aatatcttct gggtcggcgt cacactgctg acggtgcggt gaaggaggtg agcatgagtg
      721 gtctcgggca actcctggcc gtgatcgggt tcggcgcgct cgcgctgtgg tttggcgtcg
      781 ccgtcttccg cacctcctcg atggtccgct ccgcgctggc gctgctcttc gcgatggccg
      841 cgcttggcgg cctctttctc gccctggagg ccgagtttct cggcgtgctg cagctgatga
      901 tgatggcgac cgaaatggcg atcatggcca tcttcatggt gatgtacatg atggaccccg
      961 gtggtctggg tcagatggag atgacgcacc agaagcgccg tgcgctcgcc gccgggacac
     1021 tcggcgggct ggtgagcgcc gcgctcagtc tgctcactgg ctggggaccg ctcacgcgcg
     1081 cagcgcccga tccggtcgcg caagtgcacg atctcggtcg cgaactgctg gagcgctcaa
     1141 tgctgatctt cgagaccgcc ggggtgacga tcctggtggc gatggtcgcc gcgacggcgg
     1201 tcgcgatcgg gaggcgcgca tgatgacgtt cagcctgacc ctgcttgtcg gcgcggggct
     1261 cttcggcatt gggctgtacg gagcactcag ccagaccaat ctggtgatga tcatgatggg
     1321 cgtcgagttg atgctcggcg cggccctggt caacctggtc gttttctggc gcttcctcca
     1381 ccccgacgtc tatagcggcc aggtcttcgt cctgatcgtg atgacggtga tggcgctgga
     1441 gatgacggtt ggctttggcg tcgccaccgc gcggttccgc gccaaaggct cggtcgagat
     1501 ggaagaagcg gcggagctgc agggatgatc ctcgcttcgc tggcactgct cgcaccactg
     1561 gccgcagcgg gcgcgattct cgcgctgcgg cgccgggccg cggcgctcgc gctgctcggg
     1621 gcagcagtcg gcgccggggc cgcgcttggt acactggccc gcgtcgcggg ggggggcgcg
     1681 cgctgcgacg gcaatccccg gcctacccgg cctgccgctc cggctcgtgg tagacccgcg
     1741 ggcggcaagc ctcagcgcgg tcgtcgcggt cgtgacgctg ctggtcctgg tctacgccgt
     1801 ggggtatatg gcgggcgatc aggcacagcc gcgcttcgtc gccgggatga cctttttcgc
     1861 cgcggcgatg caggcgctgg tgctcgccgg cgactggctg ctcttcctga ccgcctggga
     1921 gctcatcggc ttcgcctcgt acctgctcat cggcttctgg ttcacgcgtc ccggcgtcgg
     1981 cgtggccgca acgcgcgcct tcgtcaccac gcgcgcggcc gacctcgggt tgtatgtcgg
     2041 catcttcctc ctcaccaccc acgcgggcac gaccgccatc gcgccaacac tccacgccca
     2101 gggcagcgcg gcgagggtcg cgggcctgct cctgctgctg gcggccgccg ggaaagcggg
     2161 ccaggtcccc ttccagggct ggttgcagga cgcgatggtc gggcctactc cggtctcggc
     2221 gctcctgcat tcggcggcgc tggtggcggc cggcgcggtc ctgctcatcc gcgccagccc
     2281 actgctggac ggacccgtgc tgcttgtcat cggggtggtg ggcggcacaa cggcgctggt
     2341 cacggggctg atggccctgg ctcagggcga cctgaaacga ctgctggcgt cgtcaacgtc
     2401 gagccagctc ggcttcatgt tcctcgccgt tggtgcggga gcgccggtgg ccgcgctcgt
     2461 gcacctggtc gcgcacgccg ccatgaagag cgcgctcttc ctcggggccg gtgtgttcca
     2521 gcacgcgcgc gaggcgacga cgtttgaccg gctagcgggc gtcgggcgga cgcaccgcgc
     2581 cacatatgtc ggctttggcc tggccggact cgcgctggcc ggggtgccgc cgctggccgg
     2641 cttctggtcc aaggacgccg tcgtcgccgc gaccgtcgcc tcgccgtacc actgggcgct
     2701 cgcgccgctg gcgttgctcg gcacgctgct gaccggtggt tacgtcgccc gcgccctgcg
     2761 cttgctgtgg cagggcgcgg gcgtgcagca gccggtacgt ggcctggcct ggatgggcgc
     2821 cgggctggcc gcgctgaccg ggctcgcggc agcacttggc ctggcggttc acccgctggc
     2881 tcacctgctc aacgccacgc tgccggagaa ccgcgtcgcc accctcggcg ccctggtgct
     2941 ggccgtcgct ggactcgctc ttggcgcgac tctccccatc gccaggttgc ttggtccgct
     3001 gcgcggctgg ggtgagcgcg ggtttcgtct cggcggcggc ttcgccgggc tcgtcgtgca
     3061 tcccgcactg gccctggccc gccaggctga ccggctcgac caggccatcc accggggtgt
     3121 gttcgcggtg ggccacggcg gcattgctgt cgcccgcgcc gcccgtacgc ttgaccaggt
     3181 cgacgagcgt ctggctggga gcgtcggacg cctggccctc gcgctcgcgc aggcggcacg
     3241 ctggaccgac gaggacgcga ttgatgcgct gatcgctggg ctcgtccagc gcacgcgcga
     3301 tctcggcgca cgcgccagac gcgcgcagac tggactcgtc caccaggagc tcctcgtcgc
     3361 cgccggaggc gcggcgctgc tgctcgtggt gctggtcgcc ggacttgtac ttaccgggcg
     3421 ctgatgctcg ggctggccgc ggaaaggagc cgtcgtggtg ccactcgtct cgctgaccct
     3481 ctttcttccc ctgtttggcg cgctgctcgt ggcggtctgg ccaggcgcgt ccgcacgggt
     3541 ttgccacagt gttggcctgg gggcagcagg gttgagcctg ctcggcgcga tggggatctg
     3601 gctgcgcggc gtgggcaccg ggttcggcca gatcgaggag gtccgctgga tcccctcact
     3661 cggcgcggcc taccgggtcg gcgtggatgg tatcaacctg ccgcttgtcg tgctgacggc
     3721 gatcctgttt ctcgccgcgc tggtcttctc caccaacatt accgaccggc cgcgtgccta
     3781 cgtcgccctg tttctcctgc tggagacgag ctgcctcggg gtcttcctcg cgctcgatct
     3841 ggtcctcttc tacgttttct gggaagtcac gctggtggcg atgtacttca tcattgccgg
     3901 ttggggacac gcgggccggc agcgcgcggc gttgaccttc tttctctaca cgctgcttgg
     3961 tagcctgccc ttgctgctgg ccatcctcgc cctgtacctg ggcagtcgcc cgcgcacgtt
     4021 cgatgtgcgc gcactggtcg ccgcgccgcc cctgaccggt ggcgccgaga tgctcacgtt
     4081 catcgccctg ctgctggcct tcgcggtcaa gtcgccgctc ttcccgctgc atacctggct
     4141 gccggccgcg cacgtcgagg cgccgacagc ggggagcgtc gtgctggcgg gcgtcctctt
     4201 gaaactgggc acctacggct tcatccgctt cgcactcgag ttgaccccgg gcgcgttccg
     4261 tactgccgcg ccggttgtcg cgggcatcgc ggtcttctcg gcgctctatg gcgcgttcgt
     4321 cgcgctcgcc cagaccgacc tgaaacggct ggtcgcctac accagcgtca atcacatggg
     4381 ctacgtggtg ctcggggtgg ccgcggcggc cgcggccacc gaccggggcg cgcgagcgct
     4441 ggccctcgac ggggcgacgc tccagatggt cagccacggt ctcgtcaccg gtgtcctctt
     4501 cctcctggtc ggcacggtgc aagaccgcgc gaagacacgc gagctgggcg cgctcagtgg
     4561 gctgctgcgg gtgatccccg tcatcggctg gggcttcatt ctggcggcgt tcgcgtcatt
     4621 cggcctgccc ggtctcgcgc atttcccggc cgagttccag atcttcctcg gcgccttcca
     4681 ggtctggccc tgggccgccg ccatcgtcgt gctgggcatc gcggtcacgg cgggcctcta
     4741 cctgcgcgcg atccagatcg cctttctcgg tgaagcgcct ggccgctggc aggccgtgcc
     4801 cgatctgaac gcgcgcgaag cctgggccat tctgccgctg cacatgctga ccgtggcggt
     4861 gggtcttggc cctggcgcgg tgctcgacct catccaccgg gcgacacagg cggtgggcgg
     4921 atgaccggtg cggccaccct ggcaatgggg cacgatctgg ccctgctcct cccgcaactc
     4981 ctcgtgctgg tgacggcggt cggggcgctg gtggccgaga tgttgcagcg cccgcgggcc
     5041 gcgctggtga tcacggtggc tgggctcctc gtcgccgcga tgcttgcggc gacgcgtctc
     5101 gaccagcagg tgacggtctt cct
//
LOCUS       DASUBI010000249         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_480438, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000249 DASUBI010000000
VERSION     DASUBI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480438"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..>3062
                     /locus_tag="VFU72_04810"
     CDS             165..>3062
                     /locus_tag="VFU72_04810"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEU5422839.1"
                     /translation="MLASQRDVDALKYAWLFFGAQAHLPGDDGRPFLRVALDESRAYA
                     AGIEDDLRDRAYGAISAACQALADALAAERGIPATLLSHEDLRAVYGDALALLYQLLF
                     ILYAESRELLPVEDERYRRELSLRTLIERLPGRQDVRGDQHGQREQPAAAPREIWARL
                     RRLFRALDDDTRPEYVPAFKGRLFDRDAHPLLRDLTPADGPLASMLALLGRTRDGRLI
                     DYADLTVRQLGSIYEGLLEHQAIAVAEEDLALVKGAKDKVLLIVPLTDAAGRRVLERY
                     PAGTVYLSNDKGERHAAGTYYTPEPVVRYLVDETLGPLVAGKRAEEILRLKVLDPAMG
                     SGHFLVAAVDYLARAALAAEAEAARGGVGASGGRPTATDGRPTATDGRPTATDGRPTA
                     ETADREALDSGVMALKRRIAEQCIYGVDLNESAVELAKLSLWLATAARELPLTFLDAH
                     LRAGNSLVGLAPGELDHAAEPPAPGAKRRGAKTKAAAGTKASGRATGAVAASAASGQS
                     TVGASGGRPAQPAQADLWDESGFTQDMFRLVGGRQVIDLMDSRTAEDVRAKTHVLETL
                     DPLAAPYRRLADLVVSRRFGNQMATEVFAAAAQTLLERGGVRLPEATLAPALAAARRI
                     AERQHVFHWELEFPEVFRDGLGRTRGDAAGFDALLGNPPYVSVNTLRAADPEAWAYFP
                     QVYFTTAQGKYDLYAAFVERGLALLNAHGRLAYILPNKWLTTDAGVGLRGLLAERRAA
                     HAVVDFGAYPVFPGVSNYTCLLFARREPAERISVAQRTAPQGEIALPPRAESGMWASG
                     EVEAGGLGAGVWHLAVGGAQAILDRMRDWPTLGAQATVFVGTCTNANPVFILREQGRK
                     EGIVTSWSAALGRAVRLEAELLRPVLQGRDIAPYACDDGGNRLLFPYRVREGGANLLP
                     ADELAQRYPLAWAYLNEPTVRAALEGRENGRFMGKANWYGFGYPRNMHLLGE"
BASE COUNT          460 a         1037 c         1119 g          446 t
ORIGIN      
        1 tcgatgagcg cgagcgagca gatagacccg ccgcaagcgc ccagctcttg cgtctgctcg
       61 cggcgtcaga tgccaattgg ggcattctca gcgatggtac gcgttggcgg ctctatcacc
      121 gtggcgggaa cgcgctcgaa gcgcactacg aggttgatct ggctgtgctc gccagccagc
      181 gcgacgtgga cgcgctcaag tatgcctggc tgttcttcgg ggcacaggcc catctgcctg
      241 gcgacgatgg ccggccgttt ctgcgggtcg cgctggatga gagccgcgcc tacgccgccg
      301 gcatcgaaga cgacctgcgc gaccgcgcct atggcgcgat ctcagccgcc tgccaggcgc
      361 tggccgatgc cctcgccgcc gagcgcggca tccccgccac actgctctcg cacgaggatc
      421 tgcgcgcggt ctatggcgac gcgctggcgc tgctttacca gctgctcttc atcctctatg
      481 ccgagagccg cgagctgctg ccggtcgaag acgagcgcta ccgccgcgag ctgagcctgc
      541 ggacgctgat cgagcgccta cctggcaggc aggatgtccg cggcgatcaa catgggcagc
      601 gcgagcagcc cgccgcggca cctcgcgaga tctgggcacg gctgcgccgg ctcttccgcg
      661 cgctcgacga cgacacacgg ccagagtatg tgccggcctt caagggccgg ctcttcgacc
      721 gcgacgcgca cccgctgctg cgcgacctca cgccggcgga cggcccgctg gcgagcatgc
      781 tcgcgctgct gggacgcacg cgcgatgggc gactgataga ttacgccgac ctcaccgtgc
      841 gccagctcgg cagcatctac gaggggttgc tcgaacacca ggcgatcgcc gtggctgaag
      901 aggacttggc actggttaag ggcgcgaagg acaaagtcct gctgatcgtg ccgctgaccg
      961 acgccgcggg acgccgtgtg ctggagcgct atccggccgg cacggtctat ctctcgaacg
     1021 acaagggtga gcgccacgcc gccggcacct actatacgcc tgagccggtg gtgcgctatc
     1081 tagtggacga gacgctgggt ccgctggtgg cgggcaagcg tgccgaggag atcctgcggc
     1141 tgaaggtgct cgatccggcg atgggcagcg ggcacttcct ggtggcggcg gtagattatc
     1201 tggcgcgcgc ggcgctggcg gctgaggccg aggccgctcg cggcggagta ggggcgtccg
     1261 gtggacgccc gactgcgacg gacggacgcc cgactgcgac ggacggacgc ccgactgcga
     1321 cggacggacg cccgactgcg gagacggccg accgcgaggc gctcgacagc ggcgtgatgg
     1381 cgctcaagcg gcgcatcgcc gagcagtgca tctacggcgt ggaccttaat gaatcggcgg
     1441 tcgagctggc gaagctgagc ctgtggctgg cgacggcggc gcgcgagctg ccgctgacct
     1501 tcctcgacgc gcacctgcgc gccggcaaca gcctggtcgg tctggcgccc ggcgagctcg
     1561 atcatgccgc ggagccgccg gcccctggcg caaagcggcg cggcgcgaag acgaaagcgg
     1621 ccgccggtac gaaggcgagc ggcagggcga ccggcgctgt agcggcaagc gctgcgtccg
     1681 gccaatccac cgtaggggcg tccggtggac gccctgcgca accggcgcag gccgacctgt
     1741 gggacgagag cggcttcacg caggatatgt tccggctggt cggcgggcgg caggtgattg
     1801 acctgatgga ctcgcggacc gccgaggacg tgcgcgccaa gacgcacgtg ctggagacgc
     1861 tggacccgct cgcggcgccc taccggcggc tggcggatct ggtggtgagc cggcgctttg
     1921 gcaaccagat ggcgacggag gtcttcgcgg ccgcggcgca gacgctgctg gagcgcggcg
     1981 gtgtgcggct gcccgaggca acgctggcgc ccgcgctggc cgcggcgcgc cgtatcgccg
     2041 agcgccagca cgtcttccac tgggagctgg agttccccga agtcttccgc gacgggctgg
     2101 ggcgcacgcg cggcgatgca gccggcttcg acgcgctgct gggcaacccg ccatatgtaa
     2161 gcgtgaacac cctgcgcgcc gccgatcccg aagcctgggc gtacttcccg caggtctatt
     2221 tcacgacggc gcagggtaag tacgacctct acgcggcctt cgtcgagcgc gggctggcgc
     2281 tgctgaacgc ccacggccgg ctcgcctaca tcctgcccaa caagtggctg accaccgacg
     2341 ctggcgtggg actgcgcggt ctgctggcgg agcggcgcgc ggcgcacgca gtggtggact
     2401 tcggcgcgta tccggtcttc cccggagtct cgaactacac ctgcctgctc ttcgcccggc
     2461 gcgagccagc cgagcgcata agcgtggctc agcggaccgc cccacaaggc gagatcgcac
     2521 tgccgccccg cgcggagagc gggatgtggg cgagcggcga ggtggaggcc ggcgggctcg
     2581 gtgctggcgt atggcatctc gcggttggcg gcgcccaggc catcctcgat cgcatgcgcg
     2641 actggccaac actaggtgca caggccaccg tcttcgtcgg gacatgtacg aacgccaatc
     2701 ccgtcttcat cctgcgagag caagggcgca aggaaggcat cgtcacctcc tggtcggcgg
     2761 cgctggggcg ggcggtgcgg ctcgaagcgg agctgctgcg gccggtgctg caggggcgcg
     2821 acatcgcgcc ctacgcctgc gacgacggcg gtaaccggct cctcttcccc tatcgggtgc
     2881 gagagggcgg cgccaacctg ctgcccgccg atgagctggc gcagcgctac ccgttggcct
     2941 gggcctatct gaacgagccg accgtgcgcg ccgcgctgga gggccgtgag aacggccgct
     3001 tcatgggcaa agctaactgg tatggattcg gctacccgcg caacatgcac ctgctgggcg
     3061 ag
//
LOCUS       DASUBI010000250         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1281760, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000250 DASUBI010000000
VERSION     DASUBI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281760"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFU72_04815"
     CDS             complement(<1..190)
                     /locus_tag="VFU72_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422840.1"
                     /translation="MGTYIARRLLLVIPTILAVYTITFVLMHATPGGPWDNADRPLSA
                     QAIANLNAKYHLNDPLWKQ"
     gene            complement(232..2049)
                     /locus_tag="VFU72_04820"
     CDS             complement(232..2049)
                     /locus_tag="VFU72_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5422841.1"
                     /translation="MPEQLTLREQHLLTRRRFIKLSAFLSSGAILAACGGSASSPTAA
                     STSSSSSGGSTATSAPSAAGTSAPAAAGSPAATGFASNYSQTFVINPSSAGTKLASDQ
                     SFRYMYTEPVHLDPGLATSGNEVYLIYEIWEGLVRFDNTGKAIPLAATNWDISPDGTV
                     YTFHLRDGIKWTDGTPITAQDFVWTWTRNLDPKTASEYAEALYPVKNGQAFNSGKLTD
                     ASQLGLKALDDKTFQVTLEGPAGYFLRLASTWTAMPLPKQSIEKNAEKWVEAANIISN
                     GPFKMTEWNHDQEITLTRNDGYWGDKPTLQKAVIKIVTDFATSSLQGYENDELDWATG
                     PWPADMDRIKKDAKLSKELHTVPGSQTYFVVCDSTNTGSPIGKADVRKALYLALDRQQ
                     IVEKVFQGLYIPAYTILPDDILGHNPDARIQGTVKDAQNLLAKAGYPGGKDVKLTMPF
                     SQTATNNLLAQVLQQMWQTNLGITVELQPTESKAFTALRKTFTTGHFDVYFSAWGSDY
                     LDPYDWMNFLFTTGTDFYHAHWSNQQFDQLCAKAATLQDQNERAKLYAQAEAILVGDA
                     AYIPLYHTATPYLIKPWVLDYVYQATGGRPWVNLKIAQH"
BASE COUNT          389 a          769 c          919 g          497 t
ORIGIN      
        1 attgtttcca gaggggatcg ttgaggtggt acttggcgtt gaggttggcg atcgcctgcg
       61 ccgacagcgg ccggtcggcg ttgtcccacg gcccgccggg cgtggcgtgc atcagcacga
      121 aggtgatggt gtagacggcc aggatggtgg ggatcaccag cagcagccgc cgtgcgatat
      181 aggttcccat gggcgcttct cactccctcg gccagcgctc aacgatcagg ctcagtgctg
      241 ggcgatcttc agattcaccc aggggcggcc gcccgtcgcc tggtagacgt agtccagcac
      301 ccacggcttg atcaggtagg gcgtggcggt gtggtagagc gggatgtagg ccgcgtcgcc
      361 gaccaagatc gcctcggcct gcgcgtagag cttggcccgc tcattctgat cctgcagcgt
      421 ggccgccttg gcgcagagct gatcgaactg ctggttcgac cagtgggcgt ggtagaagtc
      481 ggtgccggtg gtgaagagga agttcatcca gtcgtagggg tcgaggtagt ccgagcccca
      541 ggccgagaag tagacgtcga agtgaccagt ggtaaacgtc ttccgcagcg cggtgaaggc
      601 cttggactcc gtcggctgga gttcgaccgt gatgccgaga ttggtctgcc acatctgctg
      661 cagcacctgg gccagcaggt tgttggtggc ggtctgcgag aatggcatgg tcagcttcac
      721 atccttgccg ccagggtagc cggccttggc cagcagattc tgggcgtcct tgaccgtgcc
      781 ctggatgcgc gcgtccgggt tgtggccgag gatgtcgtcc ggcaggatcg tataggccgg
      841 gatgtagagc ccctggaaga ccttctcgac gatctgctgc cggtcgagcg ccaggtagag
      901 cgccttgcgc acgtcggcct tgccgatcgg cgagccggtg ttggtcgagt cgcagacgac
      961 gaagtaggtc tgcgagccgg ggacggtgtg cagctccttg ctgagcttgg cgtccttctt
     1021 gatgcgatcc atgtcggccg gccagggacc ggtggcccaa tccagctcgt cgttttcgta
     1081 gccctgcagc gaggaggtcg cgaagtcggt cacgatcttg atgaccgcct tctgcagcgt
     1141 cggcttgtcg ccccagtagc cgtcgttgcg agtcagcgtg atctcctggt cgtggttcca
     1201 ctcggtcatc ttgaaggggc cgttgctgat gatattggcc gcctcgaccc acttctcggc
     1261 gttcttctcg atggactgct tgggcagcgg catggcggtc caggtggagg cgaggcgcag
     1321 gaagtagccg gccgggcctt cgagcgtgac ctggaaggtc ttgtcgtcga gcgccttgag
     1381 accaagctgg ctggcatcgg tcagcttgcc cgagttgaag gcctggccgt tcttgaccgg
     1441 gtagagcgcc tcggcgtact ccgaggcggt cttgggatcg aggttgcgcg tccaggtcca
     1501 gacgaagtcc tgcgcggtga tcggcgtgcc gtcggtccat ttgatcccat cgcgcagatg
     1561 gaaggtgtag accgtgccgt cgggggagat atcccagttg gtggccgcca gcggaatcgc
     1621 cttgccggtg ttgtcgaagc gcaccagccc ttcccagatc tcgtagatca ggtagacctc
     1681 attgccgctc gtcgccaggc ccgggtcgag gtggaccggc tcggtgtaca tgtagcggaa
     1741 agactgatcg gaagcgagct tggtgccggc ggagctgggg ttgatgacga acgtctggga
     1801 gtagttcgag gcgaagccgg tcgcggcggg ggaaccggcc gcggctgggg cgctcgtgcc
     1861 cgccgcgctg ggggcgctgg tggccgtcga gccgccgctg gatgagctgc tggtgctggc
     1921 ggcggtgggg ctggaggcgc tgccgccgca ggcggccagg atggccccac tgctgaggaa
     1981 cgcggacaat ttgatgaagc gacgtcgagt gagaagatgc tgctcgcgaa gcgtcagttg
     2041 ctccggcatg atcaatccct ttcaacgctc gtctgccgtc caggcggact tacccggtct
     2101 ggcccgcggc gccgccgagt tcacacccac ccaggtgacc tcctcctcca cctgttcgcg
     2161 ccttgtcccg tgagcgggcg ccagacccgc ctcggatcac gatataatct gcccgcggcg
     2221 acagtcgagc taggtgattg gccggcgtcc gcccccgtgc gggatgccgg ctcgtgctgc
     2281 cgcgtggcgg gattccgtgg ctccctatac aactcgccgc gcaatctgac aatggccgca
     2341 atggagcctg gctgatcggc gcgtacgccg ggtttgcgga gcgcgccgcc tcgcgggccg
     2401 tcatccggac acgccgtgtg atactggttt gttgccgccg gctgtgccgg cggagagagg
     2461 agcgcaccag atgtccgaca ggccgaatcc accggagacc cggcacacga ccgaggagga
     2521 gctgcaagcc gtcaccctgg tgcccttgac gccgctcaac gggccgatcc tgct
//
LOCUS       DASUBI010000251         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1052872, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000251 DASUBI010000000
VERSION     DASUBI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052872"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFU72_04825"
     CDS             <1..801
                     /locus_tag="VFU72_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5422842.1"
                     /translation="ATQRGAQLPTATDFTALPGRGVQARVNGQLWQAGGPRLLEQAGA
                     QLPAALADAARAWGERGQTVVYLLEGDQPRAAFALADVIRPESREAVAVLQAQGVRVA
                     MLTGDAEEVARWVAGELGITEYFAQVLPEDKSARVRALQEGGALVAMVGDGVNDAPAL
                     AQADVGIAIGAGTDVARASAGIVLVKNDPRDVARIITLSRASYRKMTQNLAWAVGYNV
                     IALPLAAGVLAPIGFVLPPAVGALLMSLSTVIVALNAQTLRRLDLRAS"
     gene            1036..1653
                     /locus_tag="VFU72_04830"
     CDS             1036..1653
                     /locus_tag="VFU72_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422843.1"
                     /translation="MSEDRKLILEMLTSGRVTVEQADQLLAALTAAPAEPHELAAAPG
                     RQWQRDARTDGFVASLTPEQLIELRNHDVSGAFVRQMRATGLADLSVNDLIELHDHDV
                     TPRFVRELREAGFSGLTCHELVELRNHDIDTAFLRQMREVGLDNVTLEQLVNLHNHGV
                     DAAFVREMQDLGFDTLAPGELIELYDHGIDGPFVHGLRDPAESPE"
     gene            1789..2133
                     /locus_tag="VFU72_04835"
     CDS             1789..2133
                     /locus_tag="VFU72_04835"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEU5422844.1"
                     /translation="MSGGELIVTRLEAPHSGIVIEGAFSLGWIGCLTPEQLEFVGLLV
                     RNRGNVQKVAADLDIAYNTARNRLDEIVTALGVPAEPVDSGQAREQRTAVLRRLAAGE
                     ITSDEALRLLKE"
BASE COUNT          432 a          731 c          848 g          383 t
ORIGIN      
        1 gcgacacagc gcggggcaca actgcccacc gcgacggatt tcacggcgct gcccggccgg
       61 ggcgtgcagg ccagggtaaa tgggcagctg tggcaggccg gcgggccgcg cctgctggaa
      121 caggccggcg cgcaactgcc cgccgcgctg gcggacgcgg cccgcgcctg gggcgagcgc
      181 gggcagacgg tggtctacct gctcgaaggg gatcagccgc gcgcggcgtt cgcactggcc
      241 gacgtgatcc gcccggagag tcgcgaggcg gtcgccgtgt tacaggcgca gggcgtgcgc
      301 gtggcaatgc tgaccggcga cgcggaggaa gtcgcgcgct gggtcgctgg cgagctgggc
      361 atcacggaat acttcgcgca ggtgctgccg gaagacaaga gcgcccgcgt gcgcgcgttg
      421 caagagggcg gcgcgctggt cgcgatggtc ggtgacggcg tgaacgatgc gccggcgctg
      481 gcgcaggccg acgtcggcat cgcgatcggc gcggggaccg atgtggcgcg ggcctcggcg
      541 gggatcgtcc tggtcaagaa cgacccgcgc gacgtcgcgc gcatcatcac tctgagccgg
      601 gcgagttatc gcaagatgac gcaaaacctg gcctgggcgg tcggctacaa cgtcatcgcc
      661 ttgccgctcg ccgccggggt gctcgcgcca atcggcttcg tgctgccgcc ggccgtcggc
      721 gcgctactga tgtccctcag cacggtgatc gtcgcgctca acgcgcagac cctgcgccgc
      781 ctcgacctgc gggcgagctg acaatatgag aacacaatca gccaggagcg ttgtaacgct
      841 cctggctgat tgccttgctc ctctttcgtg agcagccacg tcacatacgg tcggggcgca
      901 gcttgactag tgcaaactcg tgcgatatta ttgctatatt gaatggcaat atttaccacg
      961 taaggtgcaa ttaccatacg gagcggagag ttcgcaggtg aatgcggcgg gtgctgcgac
     1021 cgaaagggga cgatcatgag cgaggaccgc aaactgattc tggagatgct gacgtcggga
     1081 cgtgtgactg tggagcaagc cgatcagtta ctggcggcgc tcactgctgc tccagccgag
     1141 ccgcacgaac tggccgccgc gccaggacga caatggcagc gggacgcgcg cacagacggc
     1201 ttcgtcgcca gcctgacacc cgagcaactg atcgagttgc gcaatcacga tgtgagcggg
     1261 gcattcgtcc ggcagatgcg cgcgacgggg ctggccgatc tgagcgtcaa cgacctcatc
     1321 gagctgcacg accatgacgt cacgccgcgc ttcgtccgcg aattacggga ggccggcttc
     1381 agcggcctta cctgccatga gctggtcgag ttgcgcaacc acgacattga cacggccttc
     1441 ctgcgccaaa tgcgcgaggt cgggctcgac aacgtgacgc tggagcagct ggtcaatctg
     1501 cataaccacg gagtagacgc cgcctttgta cgggagatgc aggaccttgg ctttgatacc
     1561 ctggcccctg gcgaattgat cgagctgtac gatcacggta ttgacggccc cttcgtgcat
     1621 gggctgcgtg accccgctga gtcgcccgag tgagatgaga tcggtgaatg agggctgccg
     1681 acaggtgcgg caagcagcaa cggcacacgg gccggacagg gatacggtga aatgctgaga
     1741 aagaccggaa ctcgggcact gcggcatcca ttaatcacgc gggatccggt gagcggcggc
     1801 gagttgatcg tgacacgcct ggaggcaccc cacagcggta tcgtgatcga gggcgccttc
     1861 agcctggggt ggatcggttg cctgacgccg gagcagctgg agtttgtcgg tctcctcgta
     1921 cgcaatcgcg gcaatgtgca gaaggtcgcc gccgatctgg acattgccta caacacggcg
     1981 cgcaaccgcc tcgacgagat tgtgaccgcc ctgggtgtgc cggccgaacc tgtagacagc
     2041 ggccaggcgc gggagcagcg caccgccgtg ctgcggcgcc tggccgctgg cgagatcacg
     2101 agcgacgagg cgctacgcct cttgaaagag tgagtgggta atgatcctgt acgctgggat
     2161 gggcatatgt gcaatcatcc ctggcaggag actgggcgca aattcgtagt ttgcgagaaa
     2221 cgccacacgc ccagattaag tcagtcagca gccacctgcg gcgacagcac ctcgcgccag
     2281 ctcgccgcga gttcggggca ggcggccagc gcggcggcgg cttcggggtc gcgcttgcgg
     2341 cggtgcatcg tcacgaaggc gcgggcgata gtccactccg ggtgcggccg gtcg
//
LOCUS       DASUBI010000252         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1201764, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000252 DASUBI010000000
VERSION     DASUBI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201764"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2692)
                     /locus_tag="VFU72_04840"
     CDS             complement(<1..2692)
                     /locus_tag="VFU72_04840"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEU5422845.1"
                     /translation="MANPLQKLATVGQSFWLDNLSRPLITSGELQRLIRDDGLRGITS
                     NPTIFEKALADSDDYDPEIRGLAIQGLNAEQIFEQLAMQDIREAAQLLRPVFKATEGG
                     DGFVSLELPPYLAGNTETSIREAKRLFLEIGRPNVMIKVPGTPQGIPAIRQLIADGIN
                     INITLLFSLARYQEVIEAYLAGLEARQAQGLPIDSIASVASFFVSRVDTEVDKRIDAL
                     LGETGDDDKRKKLEWLKGRVAVANAKLAYQLFQQRFSQQDARWRRLKEAGARLQRPLW
                     ASTSTKNPAYSDIKYVEALIGPDTIDTMPPATAADFRDHGVVEVNVTKDMAEQSRVLE
                     TLAETGISYDDVVETLQDQGVAAFGKSFESLLAGIEEKRKRFAGGQARGIFSDQIQAT
                     ARRLERERAVERLWQKDASLWSNDPQVQQKIKDRLGWLEVPQTMLGEVGRLNKLAEDV
                     RQAGFESAVLLGMGGSSLAPEVFQHTFGNQMGAPVLTVLDTTNPDAIQRLAGQLDLAR
                     SLFIVSSKSGTTVETSTLYRYFRQQLEQQVGADAGKHFIAVTDPGTPLEKLAKDDGFR
                     DVFVNPPDIGGRYSALSFFGLVPAAVIGLDIQHLLDRACHMAENTKTSSPQNPALQLG
                     AALGGLAQIGRDKLTFLSDPALASLQDWLEQLIAESTGKDGNGIVPVAHEPHGAPDDY
                     GSDRHFVGIDLAPQPHVDTDAMLAGLETAQQPVTRLWLRDEWDVGAEFFRWEVATALA
                     GAVLGINPFDEPNVQESKDNTNRLLQTYQQEHALPEPPAGGPDGTVAMVGNEPSSVAE
                     LVTRFLGKVRSGDYLALMAFCERSPEVEARLQEMRRLLRTQLGVATTLGYGPRFLHST
                     GQLYKGGPPQGDFLQIVVEPAEDLNIPGEPYSFGTLFGAQS"
BASE COUNT          531 a         1138 c         1219 g          603 t
ORIGIN      
        1 gcgactgggc gccgaagagc gtgccaaagc tatacggttc gccggggatg ttcagatctt
       61 cggccggctc gaccacgatc tgcaggaaat cgccctgggg tgggccgccc ttgtagagct
      121 ggccggtcga gtgtaggaag cgcgggccgt agccgagcgt ggtcgccacc ccgagctgcg
      181 tgcgcagcag acgccgcatc tcctgcaggc gggcctccac ctccggcgag cgctcgcaga
      241 aggccatgag cgccagatag tcgcccgagc gtaccttgcc caggaagcgg gtgacgagct
      301 cggccacgct gctcggttcg ttgcccacca ttgccacggt tccgtccggc ccgcccgccg
      361 gaggctcggg gagcgcgtgc tcctgctgat aggtctgtag caggcggttg gtgttgtcct
      421 tgctctcctg cacgttgggc tcgtcgaagg ggttgatgcc gagcacggcg ccggccagcg
      481 cggtggccac ctcccagcgg aagaactcgg cgccgacatc ccactcgtcg cgcagccaga
      541 ggcgcgtgac cggctgctgg gccgtctcca gtccggccag catggcgtcg gtgtcgacgt
      601 gcggctgggg cgccagatcg atgccgacga aatggcgatc cgagccgtag tcgtcggggg
      661 cgccgtgcgg ctcgtgcgcc accggcacga tgccgttgcc gtccttgccg gtgctctcgg
      721 cgatcaactg ctccagccag tcctgcaggg aggccagcgc cggatcgctg agaaaggtca
      781 atttgtcgcg gccgatctgc gccagcccgc cgagcgccgc accgagctgc agggccgggt
      841 tctggggcga ggaggttttc gtgttctcgg ccatgtggca ggcgcggtcg agcaggtgct
      901 ggatgtcgag gccgatcacg gcggcgggca ccaacccgaa gaaggagagc gccgagtagc
      961 gcccgccgat gtcgggcggg ttgacgaaga cgtcgcggaa gccgtcgtct ttcgccaatt
     1021 tctccagggg cgtgccgggg tcggtcacgg cgatgaagtg cttgccggcg tcggcgccga
     1081 cctgctgctc cagctgctgg cggaagtagc ggtagagggt cgaggtctcg accgtggtgc
     1141 cggacttcga gctgacgatg aagagactgc gcgccagatc gagctgcccg gccaggcgct
     1201 ggatggcatc gggattggtc gtatccagca cggtcagaac gggggcgccc atctggttac
     1261 cgaaggtatg ctggaaaacc tcgggcgcca ggctactgcc acccatgccc agcagcacgg
     1321 ccgactcgaa gccggcctgg cgcacgtcct cggccagttt gttcagccgg ccgacctcgc
     1381 ccagcatcgt ctggggaacc tccagccagc cgagccggtc cttgatcttc tgctgcacct
     1441 gcgggtcgtt gctccaaagg ctggcgtcct tctgccacag ccgctcgacg gcgcgctcgc
     1501 gctcgagccg gcgcgcggtg gcctggatct ggtcgctgaa gatgccgcgc gcctgcccgc
     1561 cggcgaagcg cttgcgcttc tcttcgatgc cggccagcag gctctcgaac gacttgccga
     1621 aggcggccac gccctggtcc tgcagcgtct cgaccacgtc atcgtagctg ataccggtct
     1681 cggccagcgt ctccagcacg cggctctgct cggccatgtc cttggtgacg ttgacctcta
     1741 cgacgccatg gtcgcggaag tcggcggcgg tggccggcgg catggtgtcg atggtgtcgg
     1801 ggccgatcag cgcctcgacg tacttgatgt cgctgtaggc cggattcttg gtgctggtgc
     1861 tggcccagag cggacgctgc aggcgcgcgc cggcttcctt gagacgccgc cagcgcgcgt
     1921 cctgctgact gaagcgctgc tggaagagct ggtaggccag cttggcgttg gcgacggcga
     1981 cgcggccctt gagccactcc agcttcttgc gcttgtcatc gtcgccggtc tcgcccagca
     2041 gggcgtcgat gcgcttgtcg acctcggtgt cgacccggct gacgaagaag ctggcgacgg
     2101 aggcgatgga gtcgatcggc agtccctgtg cctggcgcgc ctccaggccc gccaggtagg
     2161 cctcgatgac ctcctgatag cgcgccagcg agaagaggag ggtgatgttg atgttgatgc
     2221 cgtcggcgat cagttgccgg atggcgggga tgccctgggg ggtgcccggc accttgatca
     2281 tgacgttggg ccggccgatc tccaggaaga gccgcttggc ctcacggatg ctggtctcgg
     2341 tgttgccggc caggtagggg ggcagctcga gcgagacgaa gccgtcgccg ccctcggtcg
     2401 ccttgaagac cgggcgcagg agctgggcgg cctcccggat gtcctgcatg gcgagctgct
     2461 cgaagatctg ctcggcgttg agcccctgga tggccagccc gcggatctcg gggtcgtaat
     2521 catcgctgtc ggcgagcgcc ttctcgaaga tggtgggatt cgaggtgata ccgcgcaggc
     2581 cgtcgtcgcg gatgagccgc tgcagctcgc cggacgtgat caagggacga ctgaggttgt
     2641 cgagccagaa gctctggccc accgtcgcca gcttctgcag tggattagcc acgggagctc
     2701 cctcctcggc ggatgcgtcg tgcggcgcag ctgcgtcgcg gcggagcgac gccgccgtct
     2761 cgatgcattg taaccacagg catcgtgtcg cacatccagc tccggcacgc actatccgtg
     2821 cccgtcaggc gctgggtgtg gcggaggacg aagcggcgtg ccgcgtggga cacggatccc
     2881 tcgactgcgc tcggaatgac agggctaatg ggggctgacg acgaggacct gcatcgggcg
     2941 aggactcgcc tcgtccggga gaccccatcc cccctattat cccttcgaca agctcagagc
     3001 aggctctgcg cgcaggcgaa ggatccgtcc tcatgcggca ccatgcgttg tcgaaagcca
     3061 cctcgcccac cccgaagaac tccccagaac caatcggcca aagggggttg acaaatcgtc
     3121 cagcgtacgt acaataaggt cagggtgtac gtacaacgtg agaggaggga cgcttggacg
     3181 cacgagtcgg aagacgctcc cgccggcatc ccagtcgcta ctcgcgttgc cgccgtctca
     3241 tggagcggca ggtacgtccc ggagccgacc tgacctggac tcgcgagctg ccggccggcg
     3301 ccgtggggca gcggaagtcg gactacagca tcagttgccc ggccagccgc gactggctcc
     3361 tggctcgcag ccgccgtttg aagagtttcc acgcgcgctg ctggccaagc tgaaccacga
     3421 accatctctt ccctccctcc ctgtacgggg ccgtcccaga cggccccttt ttgatttctc
     3481 tagaccggtc a
//
LOCUS       DASUBI010000253         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_57502, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000253 DASUBI010000000
VERSION     DASUBI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57502"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFU72_04845"
     CDS             <1..752
                     /locus_tag="VFU72_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422846.1"
                     /translation="AVAIEARSQFNPATDVLPWANSTASLGTVEPRWDIFLRTFGLAL
                     LPHAFFRGLYRAMVHLDPRGGGLCSGLAHLALERSLKPTSAQPTIDDVLRWHGRQLSD
                     RATLASAPWFLRPSPRRAYRAFRAQLLAQGWSDICFDIAQPSLRHPRVGRVLTNGHTV
                     LPYALRQESPERAEVLVYDPNDPQAAREDRAVISFDLAGDSYAYPGHVAPHDGTTIYA
                     PRQRSYRGGRTALLASLASLVLFPRSFKKDR"
     gene            complement(1066..1392)
                     /gene="trxA"
                     /locus_tag="VFU72_04850"
     CDS             complement(1066..1392)
                     /gene="trxA"
                     /locus_tag="VFU72_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5422847.1"
                     /translation="MAKPEAVTDATFNQSVVQSDKPVLVDFWAEWCGPCRMVAPILDE
                     IAREKDGELKIAKVNVDQDGRIAQSLGIVSIPTLILYKDGKPVERIVGAQPKQRLLAQ
                     IEKHLS"
     gene            1484..>2008
                     /locus_tag="VFU72_04855"
     CDS             1484..>2008
                     /locus_tag="VFU72_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5422848.1"
                     /translation="MSGATPLIGLDGFFLSQPTTGSGQYTVNLWRELADHDHRLASVL
                     LCPATREYESVKGPAQRLVVPARLAAPKSRKLWWEQRGVLRAARLARASLLHVPYFSA
                     PRYARLPVVTTIHDVIPLLFPEYGGSPAMQLYLRLVSAAARRARLVLTDSDCSRRDIT
                     RLLGIPEARIRVVPL"
BASE COUNT          290 a          731 c          646 g          341 t
ORIGIN      
        1 tcgcggtggc gatcgaggcg cgcagccaat tcaacccggc caccgatgtg ctaccctggg
       61 ccaacagcac cgcttcactg ggcaccgtcg agccgcgctg ggacatcttt ctccgtacct
      121 ttggtctggc gctgctgccg cacgccttct ttcgcggact gtaccgcgcg atggtccacc
      181 tcgacccgcg cggcggcggc ctctgcagcg gcctggccca cctggcgctg gagcgcagcc
      241 tcaagcccac gagcgctcag ccgaccatcg acgacgtgct tcgctggcac ggccggcagc
      301 tctccgaccg cgccacgctg gcctcggcac cctggttcct gcggccgagc ccacgccgcg
      361 cgtaccgggc cttccgcgcg caactgttgg cgcagggctg gagcgacatc tgcttcgata
      421 tcgcccagcc gagcctgcgc catccccggg ttggtcgcgt gctgacaaac gggcatacgg
      481 tactccccta cgcgctgcga caggagtcgc ccgagcgggc cgaggtgctg gtctacgacc
      541 ccaacgaccc gcaggccgcg cgcgaggacc gcgccgtgat cagcttcgac ctcgccggcg
      601 acagctacgc ctacccgggc cacgtggcgc cgcacgacgg caccacgatc tacgccccgc
      661 gccagcgcag ctaccgcggc gggcgcacgg cgctgctggc cagtctggcc agcctggtgc
      721 tctttccgcg ctcgttcaag aaagatcggt gactgaagca gaacgttggg tgtcggggca
      781 gcacggaccc ctccactgcg gtcgggatga cagagttaag aaggggcgtg cgagtacgac
      841 gcgacgcact cgtacgcccc atattttggc ccgtcaacct gaacctgtcg aaggattcgt
      901 cctcgcgcga cacccgacga agaagggcag gttcgaaacc cgcccctaca accgtagccc
      961 cacccttctc ccgcgcaccg ggagacaggc cggggatgag ggttcccccg ccgccagtca
     1021 gaaggagccc gcgcggtctg tgcgccgcgc gggtggtacg ccgggttagc tgagatgctt
     1081 ctcgatctgg gccagcaggc gctgcttcgg ctgcgcgccg acgatgcgct cgaccggctt
     1141 gccgtccttg tagaggatca atgtcgggat gctgacgatc ccgagcgact gggcgatgcg
     1201 cccgtcttga tcgacgttca ccttggcgat cttgagctcg ccgtccttct cgcgcgcgat
     1261 ctcgtccagg atcggggcga ccatgcggca gggaccgcac cactcggccc agaaatcgac
     1321 gagaaccggc ttgtccgact gcaccacgct ctggttgaac gtggcgtcgg tcaccgcttc
     1381 gggtttggcc atcgatgtct ccttcgtctc accccggcgc catccgctct ggcaccggtg
     1441 catgtatcgc aacgttcgcg ccgtctgtta taatccgccc gtcatgagcg gcgccactcc
     1501 tctgattggc ctcgatgggt tctttctctc ccagccgacg accggaagcg gtcagtacac
     1561 cgtcaacctg tggcgcgagc ttgccgacca tgaccatcgt ctcgcgtcag tcctcctctg
     1621 tccagcgacc agggagtacg aaagtgtgaa aggtccggcc cagcggctgg tggtgccggc
     1681 gcggctggcc gcgcccaagt cgcgcaagct ctggtgggag cagcggggcg tgctgcgcgc
     1741 ggcccggctg gcgcgggcca gtttgctgca cgtgccctac ttctcggcgc cccgctacgc
     1801 gcggctcccg gtcgtgacca cgatccacga cgtgatcccg ctcctcttcc ccgagtacgg
     1861 cggctcgccg gcgatgcagc tctacctgcg gctggtcagc gcggccgcgc gccgggcccg
     1921 gctggttctg accgactccg actgctcgcg gcgggacatc acgcggctct tgggcatccc
     1981 cgaggcgcgc attcgcgtgg tgccgctg
//
LOCUS       DASUBI010000254         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1147726, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000254 DASUBI010000000
VERSION     DASUBI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147726"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1693
                     /locus_tag="VFU72_04860"
     CDS             185..1693
                     /locus_tag="VFU72_04860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5422849.1"
                     /translation="MSTIGAQVETLRNYVGGQWIEAAASETQEVRNPATDELLAKVPL
                     GNAADVDAAVAAAKAAFPAWRATPPQERARYFFKLRTLVEEHADELARLITTEMGKTL
                     DDARGEVQRAIENIETACGIPTLMMGYGLEDGAARGIDEEVIYQPLGVFAAITPFNFP
                     FMIAFWFWPYAVGCGNTYIVKPSEQDPIVQRRVFELIDQVGFPPGVINLVNGGKEAVN
                     SLLDHPDVRGISFVGSTATAQYVYARASASGKRVQASGGAKNAIVVMPDADLDEYLST
                     IMNSCFGAAGQRCLANSLILPVASAHDTVVERVADAAGKLRVGDGLASGVELGPVVSA
                     AARDRIFAAIGGAVDEGASAVLDGRQVNVPDRPHGYFVGPTVLDALHPSMTAYQQEIF
                     GPVVGVSQVGSLDEAIARVNAADYGNAASIFTQSGAAAREFRYRVEAGNIGINVGVAA
                     PMAYFPFSGAKRSFFGTLHPQSQDAVRFFTESKVVISRWTTGSKGPVTGIGR"
     gene            complement(1690..>2283)
                     /locus_tag="VFU72_04865"
     CDS             complement(1690..>2283)
                     /locus_tag="VFU72_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5422850.1"
                     /translation="IVSALNRSATENLNGQAFQLIGLIQTSAPINPGNSGGALVNLQG
                     QLIGIPTLGAVNPESGAAAPGIGFAIPSNRVKFVMDQLIKNGRLVNSGQGFIGIQGED
                     VTPDLASAYNLPAQSGVLVAGFAPDSSGNSPAQQAGVKQGDIITAVNGKAVANSGDLA
                     GALESQSPGTKVTLTVVRSSGTQQITVTLGQRPASGS"
BASE COUNT          351 a          768 c          802 g          362 t
ORIGIN      
        1 cgcgacactc gatcgcgcgc tggaagctgc tgagcgcgca taaccgacac atcgcgggcg
       61 tatatcccag tccgcggagg cggacttcgt ggccgcaagg ccccataggc gcggtttcaa
      121 ccgccggccg gctgtcggcg cggattcatc caccgggcga atagaaccag gaggcaggca
      181 taccatgagc acgattggcg cgcaggtcga gacgctgcgc aactacgtcg gcggccaatg
      241 gatcgaggcg gcggcgagcg agacgcaaga ggtgcgcaat cctgccaccg acgagctgct
      301 ggcgaaggtc ccgctgggca acgcggccga cgtggatgcc gcggttgccg ccgcgaaggc
      361 ggccttcccc gcgtggcgcg cgaccccgcc acaggaacgc gcgcgctact tcttcaagct
      421 gcgcacgctg gtggaggagc acgccgacga gctggcccgc ctgatcacca ccgagatggg
      481 caagacgctc gacgacgccc gcggcgaggt gcagcgggcg atcgagaaca tcgagacggc
      541 ctgcggcatc cccaccttga tgatgggcta cgggctggag gacggcgccg cgcgcggcat
      601 tgacgaggag gtcatctacc agccgctggg ggtcttcgcg gccatcacgc cgttcaactt
      661 cccctttatg atcgccttct ggttctggcc ctacgccgtg ggctgcggca acacctacat
      721 cgtcaagccc tccgagcagg accccatcgt gcagcggcgg gtcttcgagc taatcgacca
      781 ggttggcttc ccgccggggg tgatcaacct cgtcaacggc ggcaaagagg ccgtcaactc
      841 cctgctcgac caccccgacg tgcgcggcat ctcgttcgtc ggctcgacgg ccaccgcgca
      901 atacgtctac gcccgtgcct cggccagtgg caagcgtgtg caggcctccg gcggggccaa
      961 gaacgccatc gtcgtgatgc ccgacgccga cctggacgag tatctctcca cgatcatgaa
     1021 ctcgtgcttc ggcgccgccg gccagcgctg cctcgccaac tcgctgatcc tgcccgtcgc
     1081 gagcgcgcac gataccgtcg tggagcgcgt ggcggatgcc gccggcaagc tgcgcgtggg
     1141 cgatggactg gcgtccggcg tggagctagg gccggtggtc tcggccgccg cgcgcgaccg
     1201 tatcttcgcg gcgatcggcg gcgcggtgga tgagggcgcc tcggcggtgc tcgatgggcg
     1261 ccaggtgaac gtgcccgacc gcccccacgg ctacttcgtc gggcccacgg tgctcgacgc
     1321 gctgcatccg agcatgacgg cctaccagca ggagatcttc ggtccggtgg tcggcgtctc
     1381 gcaggtcggc agcctggatg aagccatcgc gcgggtcaac gcggcggact acggcaacgc
     1441 ggcctcgatc ttcacgcaga gcggcgccgc ggcgcgcgag ttccgctacc gtgtcgaggc
     1501 gggcaacatc ggcatcaatg tcggcgtcgc cgcgccgatg gcctacttcc ccttctccgg
     1561 cgccaagcgc tcgttctttg gcacgctgca cccacagagc caggacgccg tgcgcttttt
     1621 cacggagagc aaggtggtca tcagccgctg gacgacgggc agcaaaggcc cagtcacagg
     1681 gatcgggcgc tagctgccgc tggcggggcg ctggccgagc gtgaccgtga tttgctgcgt
     1741 gccggagctg cgcacgaccg tgagcgtgac cttcgtgccc ggcgactggc tttcgagcgc
     1801 gccggccagg tcgccgctgt tggcgaccgc cttgccgttg acggcggtga tgatgtcgcc
     1861 ttgcttcacg ccggcctgct gcgcgggact gttgccggac gagtcggggg cgaagccggc
     1921 caccagcacg ccgctctgcg ccggcaggtt atacgcgctg gcgagatcgg gcgtcacgtc
     1981 ctcgccctgg atgccgatga agccctggcc agagttgacg agtcgcccgt tcttgatgag
     2041 ctggtccatc acgaacttga cgcggttgga ggggatcgcg aagccgatgc caggggcggc
     2101 cgcgcccgac tcgggattca ccgcgcccag cgtggggatg ccgatgagct ggccttgcaa
     2161 gttgaccagc gcgccgccgc tgttgcccgg attgatgggc gcgctggtct ggatcaggcc
     2221 gatcaattgg aaagcctggc cgttgaggtt ctcggtcgcg gagcggttga gcgcgctgac
     2281 gat
//
LOCUS       DASUBI010000255         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_57399, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000255 DASUBI010000000
VERSION     DASUBI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57399"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1380
                     /locus_tag="VFU72_04870"
     CDS             190..1380
                     /locus_tag="VFU72_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5422851.1"
                     /translation="MKLLVVTPHLPAPPESGGARRMFELIRQLAARHDVVTLSLAGPD
                     EDVALNERELGRVVAVRVPATARMPPSAAKRRTQLRSLLSPRSFHHYFYRQAALQRRL
                     RELLTAERFDLVQFEYSAMGGYDAPGLPRVVDVVDIEYDVLGQQAREGSIARRIFNAV
                     EARKFAGEERAAWCAATVCLATSEQDAERIAAITGGRVAVIPNGVDTGYFQRRPLDGT
                     DPRDLVFVGALRYRPNAEAVLFFVEQVMPLLRRSVPGVSLTVVGADPPPEISALCGPD
                     VQIAGTVPDVRPHLAAAGTVVVPLHVGGGTRLKILEAFAAGRPVISTRLGAAGLAVRD
                     GEELLYAEEPAEFVAALRRLIEQPELRQRLAGNGHALVRARYEWAAIVRRLDDAYQAI
                     CSGA"
     gene            1496..>3901
                     /locus_tag="VFU72_04875"
     CDS             1496..>3901
                     /locus_tag="VFU72_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5422852.1"
                     /translation="MRDSESPFRPNDHGRTNEPLVGREREQALLRQALDAMLAGHGGL
                     MLVSGEAGIGKTTLVEWLAGEAFGTGASVLWGHAYDLSATPPYGPWIEIARRYPASTQ
                     PASFPNFLDDPRHASAIGSQDQVFSQVTSFLTHLSDERPLVLILDDLHWADQASLDLL
                     RVVAREAHAHRLLLLGTCRSEELTRRHPLFQLIPLLVREAHAERLVVRPLDDEATRAL
                     IEARYPLSRADVARLGTYLRARSEGNPLYALELLRTLEDDDILSAPTGGWQLGDLEHV
                     RVPQLLLQMLERRAGRLTQEARGLLQFAAVIGHDVPLDLWQEASGAADDALARAIDQG
                     LETHLLVENASDAVRFGHALMREALYAEVITPRRRSLHQKVAETLERAPHPDPDAVAY
                     HYQQAGDGRAARWFIKAGERAEDSYAWVTAVERFEVARALLERQRVDAAEYGWLLLRI
                     AAIRRYERPSRAMEILDAAAAYAESARDPVLFAYFLLHRGQLRCLEGQVELGLADLEA
                     GVAAVRGLAPDQRARLGALPPNVLWAVDGDGLGTLVFHLARSGRYHAARALGEQFASE
                     HAQHGASIAPLSDRGDVSCGLAIAYAALGRPDDAREAFRHARAAYSRLGHHLLVADAL
                     EFELNMVHLPYESDDLHWRSRLLLAGEQALDRSLGASFYDSLRDFDLSVRILEGDWAC
                     ARAFVQANPSRLLHQFALASVGYAQGDTELAIAVVRAAFPDGAATAPGNKRFFYALPM
                     LRLAAEIAFDTGDLPNAQTWLDAHDRWLDWSGAVLGRAEGAVLWARYHHVNGDHEGAQ
                     RLVG"
BASE COUNT          559 a         1367 c         1355 g          620 t
ORIGIN      
        1 gacgactgtg gcaagtcgta gtcgaaggcc acaaacgcta agctgtcatc ctgagcgcag
       61 tcgaaggatc cgtcttgccc tagcgcaata ccgactggtt cgccacctcc cctctcccgc
      121 gcacggctcc gtccggagag gggccggggg tgagggcact cccgccgggg gtgagggccc
      181 ggccacacca tgaagctcct cgtcgtcacg ccgcacctgc cggcaccacc cgagtcgggc
      241 ggcgcgcggc gcatgttcga gctgatccgc cagctggcgg cgcggcatga tgtcgtcacg
      301 ctcagcctgg ccgggccgga cgaggacgtg gcgctcaacg agcgggagct gggacgagtg
      361 gtggcggtgc gcgtgccggc caccgcccgc atgccgccca gcgccgccaa gcgccgcacg
      421 cagctccgct cgctcctctc gcctcgctcc ttccaccact acttctatcg ccaggccgcg
      481 ctgcagcgcc gcctgcgcga gctgctgacc gccgagcgct tcgatctggt ccagttcgag
      541 tactcggcca tgggcggcta cgacgcaccg gggctgccca gagtggtgga tgtcgtcgat
      601 atcgagtacg acgtccttgg ccagcaggcg cgcgagggtt ccatcgcacg gcgcatcttc
      661 aacgccgtcg aggcgcgcaa gttcgccggg gaggagcgcg ccgcctggtg cgcggccacg
      721 gtctgcctgg cgacctcgga gcaagacgcg gagcggatcg cggcgatcac cggcgggcgg
      781 gtggcggtca tccccaacgg cgtcgacacc ggctacttcc agcgccggcc gctggacggc
      841 accgacccgc gcgacctcgt cttcgtcggt gcgctacgct accggcccaa cgccgaggcg
      901 gtcctcttct tcgtcgagca ggtcatgccg ctgctgcgcc ggtcggtccc cggcgtctcg
      961 ctgaccgtcg tgggcgccga cccgccgccc gagatcagcg cgctctgcgg cccagatgtc
     1021 cagatcgccg gcacggtgcc cgacgtgcgg ccccacctgg cggcagcggg cacggtggtc
     1081 gtgccgctgc acgtcggcgg cggcacgcgg ctgaagatcc tggaggcgtt cgcggcgggc
     1141 cggccggtga tctcgacccg gctcggcgcg gcggggctgg ccgtgcgcga cggcgaagag
     1201 ctcctctacg ccgaggagcc ggccgagttc gtggccgctc tgcgacgcct gatcgagcag
     1261 ccggagctgc gccagcggct ggccggcaac ggccacgccc tggtgcgcgc ccgctacgaa
     1321 tgggccgcca tcgtgcgccg cctcgacgat gcctaccagg cgatctgctc gggtgcgtag
     1381 gcgcaccgcg atcgcgcgcg ctcaggtggc ttgacgctgg tcctctgttt gacgaaaatg
     1441 cgagctgcgg gccgtatctc ttgctgagtt caccaagggc tgcggggcat tgactatgcg
     1501 cgactccgag tccccgttca ggccaaatga tcacggccgc accaacgagc ccctcgtcgg
     1561 ccgcgagcgc gagcaggcgc tcctgcgcca ggcgctcgac gcgatgctgg ccgggcacgg
     1621 cggtctgatg ctggtcagcg gtgaggccgg catcggcaag accactctgg tggagtggct
     1681 ggccggggaa gccttcggaa ccggagccag cgtcctctgg gggcacgcgt acgacctgtc
     1741 ggccaccccg ccctacggcc cctggatcga gatcgcccgg cgttacccgg cgagcaccca
     1801 accggcgtct tttccgaact ttctcgacga cccgcggcac gcgagcgcca ttggcagcca
     1861 ggaccaggtc ttcagccagg tgacgtcgtt tttgacccac ctgtccgatg agcgtccgct
     1921 ggtgctcatc ctcgacgacc tgcactgggc cgatcaggcc agcctcgacc tcctgcgcgt
     1981 cgtcgctcgt gaggcccacg cgcatcggct ccttctgctc ggtacctgcc gcagcgagga
     2041 gttgacccgt cgccatccgc tcttccagtt gattccactt ctcgtgcgcg aggcgcacgc
     2101 cgagcggctg gtggtgcggc cactggacga cgaggcgacg cgcgcgctga tcgaagcacg
     2161 ctatccgctg tcccgcgccg acgtcgctcg cctgggcaca tacctgcggg cgcgatcgga
     2221 gggaaacccg ctctacgccc tcgaactgct acgcacgctg gaagacgacg acatattgag
     2281 cgcgccgacc ggcggctggc agctgggcga cctggagcac gtgcgggtgc cgcagttgct
     2341 gctgcagatg ctggagcgcc gcgccgggcg gctcacccag gaagcgcgtg ggctgctcca
     2401 attcgcggca gtcattgggc acgatgtgcc gcttgatctg tggcaggagg cgagcggagc
     2461 agcagacgac gcgctggctc gggccatcga tcagggactg gagacgcatc tgctcgtgga
     2521 gaacgcgagc gatgcggtgc gcttcggcca cgcgctgatg cgtgaggccc tgtatgcgga
     2581 ggtgatcaca ccgcggcgcc ggagcctgca tcagaaggtc gcggagacgc tggagcgcgc
     2641 gccacacccc gacccggacg ccgtcgccta ccactaccag caggcaggcg acgggcgtgc
     2701 ggcgcggtgg tttatcaaag cgggcgagcg ggcagaagac agctatgcct gggtaaccgc
     2761 ggtcgagcga ttcgaggtgg cccgcgcgct cttggagcgg cagcgcgtcg atgccgccga
     2821 gtatggctgg ctcctcctgc ggattgccgc cattcgccgg tacgagcgcc cgtcgcgggc
     2881 tatggagatc ctcgacgccg ccgcggccta tgcggaatcg gcgcgggacc cggtgctctt
     2941 tgcctacttc ctgctccatc gtggtcagct ccggtgtctg gaagggcagg tcgaattggg
     3001 gctcgccgat ctggaagccg gggtcgcggc ggtgcgcggt ctcgcacctg atcagcgggc
     3061 gcggctcggc gccctgccgc ccaacgtgct ctgggctgtg gacggggacg ggctcggcac
     3121 gcttgtcttt cacctggctc gctctggacg ctaccatgcc gcacgagcac tcggcgagca
     3181 gttcgccagc gaacacgccc aacacggcgc ctcgatcgct cccctcagcg atcgcggtga
     3241 tgtgtcctgc gggctggcga tcgcctatgc ggcgttggga aggcccgatg acgcgcggga
     3301 agccttccgc cacgctcgcg cggcgtacag cagactcggc catcacctcc tggtcgcgga
     3361 cgccctcgaa ttcgaactca acatggtcca tctgccgtat gagtccgacg atctgcactg
     3421 gcgatcgcga ctgctgctgg ctggcgagca ggcgctcgac cgatcgctgg gggcgtcatt
     3481 ctacgactcc ctccgcgact tcgacctgtc ggtacggatc ctcgaaggcg actgggcatg
     3541 cgcgcgtgcc ttcgtgcaag cgaacccgtc gcggctgctc caccaattcg ctctcgcctc
     3601 ggtgggctac gcgcagggtg acacggagct ggccatcgcg gttgtccgcg ccgcgtttcc
     3661 cgatggcgcg gctaccgcac ccggaaacaa acgcttcttc tacgcgcttc cgatgctgag
     3721 gcttgccgcc gagatagcgt tcgacactgg cgacctgccg aacgcgcaga cctggctcga
     3781 cgcccacgat cgctggctgg attggtcggg agccgtactc ggccgggccg aaggcgctgt
     3841 gctgtgggcg cggtaccacc acgtcaacgg agatcacgaa ggcgcccagc gactggtcgg
     3901 g
//
LOCUS       DASUBI010000256         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_139595, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000256 DASUBI010000000
VERSION     DASUBI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139595"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFU72_04880"
     CDS             complement(<1..464)
                     /locus_tag="VFU72_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422853.1"
                     /translation="MIRILVVDDHPIVREGLVAVLDAQDDMTVVGEASDGEQALRIYK
                     AARPDVVLMDLAMPQLDGVEAIEAIRAFDHDARVVVLTAYDTDERILRAVEAGARGYL
                     LKGAPRDEIYRAVRVVNLGGSLLEPGIAGKLLNRVGDILRGEIPEEELTPRE"
     gene            complement(461..>2530)
                     /locus_tag="VFU72_04885"
     CDS             complement(461..>2530)
                     /locus_tag="VFU72_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5422854.1"
                     /translation="ARVIPGHNFLISFYDAPSANLSPRLLYFQGIRRPELEQHPRPAG
                     LIRVMAETQQPLAVLDYVAACAEHGVQPDPLVLQSPQPLHFMAAPMIQGGQTVGVIAC
                     FSPHLPFQPEDLELLAAAARQTAIALEIRRLIKAERKRVNQLRAVNQLAREIVARQDA
                     PTLLQTAADLVHELFGYNLVAILLREGTDELVMRASAPRPGEVAPASLRLPIDEHSIV
                     GSVAATGAAALVGDVAADPRYLATPQTGSTRAELAVPLARDGRVLGVLDVQSPELHAF
                     DRHDLTTLATIADQIVVALQNAELLQDERERSRALALMLNTTRAAGSSLITDEVLEHL
                     AGGIVDAAAARACAIYIFDPESKSFSPATVVGDWTLGRPTFAVERNPFLQRLLSQQQP
                     FASCTLGAFSNNSDCACHGAPLLLVPLIARGQTLGLALVVAGDEHDFSPETVRLVQGV
                     ADSAALTVENARLYARSQGLTIAEERGRLAQEIHDGLAQGLTAISLQLDLADTFLPAK
                     PAKALEKVRRALELTRENLEDARRSVLDLSASRLHEVPLPEALRRLALGSSRAAGVPV
                     DVFTEGLSGRLSARVETGLYRIAEEALENTRRHARAHAVRLSLQASDGVVRLTIEDDG
                     VGFDPSQTARSLRPGSGFGLVGIRERARLLKGRLELESAAGAGTRLIVTVPYEASRRA
                     EKMENEA"
BASE COUNT          362 a          907 c          896 g          365 t
ORIGIN      
        1 tgctcacgcg gggtcagctc ctcttcgggg atctcgccgc gcaggatatc gcccacccgg
       61 ttgagcagct tgccggcgat ccccggctcc agcagcgagc cgccgagatt gaccacgcgc
      121 acggcgcgat agatctcgtc gcgcggcgcg cctttgagca ggtagccgcg cgcgccggcc
      181 tcgaccgcgc gcaggatgcg ctcgtcggtg tcgtaggcgg tcagcaccac cacgcgcgcg
      241 tcgtggtcga aggcgcgaat ggcctcgatc gcctcgacgc cgtcgagctg aggcatcgcc
      301 aggtccatca gcaccacgtc gggccgcgcc gccttgtaga tgcgcagcgc ctgctcgccg
      361 tcgctggcct cgccgaccac cgtcatgtcg tcctgagcgt cgaggacggc gaccagcccc
      421 tcccggacaa tcgggtgatc atccaccacc agaatacgaa tcatgcttca ttctccattt
      481 tttccgctct acggcttgct tcatagggga cagtcacgat cagccgcgtg ccggcgcccg
      541 ccgcgctctc caattccagc cggcccttga gcagccgggc gcgctcgcgg atgcccacca
      601 ggccgaagcc gctgcccggc cgcagcgagc gggccgtctg gctgggatcg aagcccacgc
      661 cgtcgtcctc gatcgtcagc cgcaccaccc catcgctggc ctgcagcgac agccgcaccg
      721 cgtgcgcccg ggcgtggcgg cgcgtattct ccagcgcctc ctcggcgatg cggtagagcc
      781 cggtctcgac ccgcgccgag agccgcccgc tcagcccctc ggtgaagaca tcgaccggca
      841 cccccgcggc tcggctactc cccagcgcca ggcggcgcaa cgcctccggc agcggcacct
      901 cgtgcagccg cgaggcgctc agatcgagca ccgaacgccg cgcgtcctcc agattctcgc
      961 gtgtcagctc cagcgcgcgg cgcaccttct ccagcgcctt ggccggcttg gcgggcagga
     1021 aggtgtcggc cagatcgagc tgcagcgaga tggcggtcag gccctgggcc agcccatcgt
     1081 ggatctcctg cgccagccgc ccgcgctcct cggcgatcgt cagtccctgc gagcgggcgt
     1141 agaggcgcgc gttctcgacc gtcagggcgg cggagtcggc cacgccctgc accagccgca
     1201 ccgtctccgg gctgaagtcg tgctcgtcgc cggccaccac cagcgccagc ccgagcgtct
     1261 gcccccgcgc gatcagcggc accagcagca gcggcgcgcc gtggcaggca cagtcggagt
     1321 tgttcgagaa ggcgcccagg gtgcagctgg cgaagggctg ctgctggctg aggagacgct
     1381 ggaggaaggg gttgcgctca accgcgaagg tggggcggcc gagggtccaa tcgcccacca
     1441 cggtcgccgg ggagaagctc ttgctctcgg gatcgaagat atagatggcg caggcgcggg
     1501 cggcagcggc gtccacgatt ccgcccgcca ggtgttcgag cacctcgtcg gtgatcaggc
     1561 tggagccggc ggcgcgggtg gtattgagca tcagggccag cgcccggctg cgctcgcgct
     1621 cgtcctgcaa cagctcggcg ttctgcaagg ccaccacgat ctgatcggcg atcgtcgcca
     1681 gcgtggtgag gtcatggcgg tcgaaggcgt gcaattcggg gctctgcacg tcgagcacgc
     1741 cgagcacccg gccgtcgcgc gccagcggca ccgccagctc cgcgcgcgtg ctgccggttt
     1801 gcggcgtagc caggtagcgc gggtcggcgg cgacatcgcc caccagggcc gccgcgccgg
     1861 tggcggccac gctgccgacg atgctgtgct cgtcgatcgg caggcgcagc gaagcgggcg
     1921 cgacctcgcc gggccggggc gcgctggcgc gcatgaccag ttcgtcggtg ccctcgcgca
     1981 gcaggatcgc caccaggttg tagccgaaga gctcatgcac cagatcggcc gcggtctgca
     2041 gcagcgtcgg cgcgtcctgc cgcgccacga tctcgcgcgc gagctggttg acggctcgca
     2101 actggttgac gcgcttgcgc tcggccttga tcagccgccg gatctccagc gcgatcgcgg
     2161 tctgccgtgc ggcagcggcc agcaactcca gatcctcggg ctggaagggc aggtgcggcg
     2221 agaagcaggc gatgacgccg acggtctggc cgccctggat catcggggcg gccatgaaat
     2281 gcagcggctg cgggctctgc agcaccagtg ggtcgggctg gaccccgtgc tcggcgcagg
     2341 cggccacgta gtccagcacc gccagcggct gctgcgtctc ggccatcacc cggatcagcc
     2401 ccgccgggcg tgggtgctgc tccagctcgg gacggcggat gccctggaag tagaggaggc
     2461 gcggcgagag attggcgctc ggggcatcgt agaaggagat caggaagttg tggccgggga
     2521 tgacgcgtgc
//
LOCUS       DASUBI010000257         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_984282, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000257 DASUBI010000000
VERSION     DASUBI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984282"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFU72_04890"
     CDS             complement(<1..903)
                     /locus_tag="VFU72_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEU5422855.1"
                     /translation="MNALERRTADATAAQTSQSTDAYQPGDYYDVAALKRANPLDQVA
                     LDYGLRLLPAGERRFWALCPFHNDRRRPNLRLDLRDAGDEHFHCFAGQCRAHGDVIHF
                     VMRLEGLDFRAACTLLARRAGLPDTYRQRAAGGRGAALSRPLRRWDHLSLDEQTVMNL
                     AGAIYRRMLWQHTDALRYLQARGLPRWLIRRCALGWADGVTLAGVLARHRDPRLLPIA
                     LDLGLLRRGPSGRPCDALAGRIVVPEVRNGHYLWFIGRGLSASGGTTLAGAKYLALPG
                     ERPVLGQQWAARRPVVYACEGVFDW"
     gene            complement(900..2099)
                     /locus_tag="VFU72_04895"
     CDS             complement(900..2099)
                     /locus_tag="VFU72_04895"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU5422856.1"
                     /translation="MLFGLAHQSLLIFDLFDRSLTNANMVVIATSGAGKSFCLKLLVL
                     RQLAEGVGSFIIDPDDEYRRLCAAVGGQYIRLALASRQAINPLDLPLPSTQDDDEPRD
                     PVAEQVASVLTLLELLLADPGRQLAAAERALLDRALYATYAASGITPADPTSWSRPAP
                     LLGDLAAELTAQPGDLAASLVVRLERYVSGSLAGLVNRRTNVSLDGRLVVFSIQQLEK
                     DLQPLAIQIITAYIWGRIRRDRQPRLLVVDEAWKLLQHPAGADFLQAMYRRARKYELG
                     VVALTHRAEDFLATDHGAAILANTAVKLLLKQTETSVRTVAQLFALTSGEERDLLGAR
                     KGEGLFFARGSRVALTVVACPEEHDLATSDPREVAALEAAAHTEAASAALPSGTSVSF
                     RPEGRQR"
BASE COUNT          363 a          707 c          776 g          319 t
ORIGIN      
        1 ccagtcgaag acgccctcgc aggcgtagac caccggccgc cgcgccgccc actgctgccc
       61 gagtacgggg cgctcgccag gcaacgctag atacttcgcg cccgcgagcg tggtgccgcc
      121 acttgcgctg aggccgcgcc cgataaacca gagatagtgg ccgttccgca cttccggcac
      181 gacgatccgc ccagccagcg catcgcatgg tcggccactg ggtccgcgtc gcaagagacc
      241 caggtcgagc gcgatcggca gcaaacgtgg atcacggtgc cgtgcgagga caccggcgag
      301 cgtgacgcca tcggcccagc cgagtgcgca gcggcggatc agccagcggg gcagaccgcg
      361 tgcctggaga tagcgcaggg cgtcggtgtg ctgccagagc atgcgccggt agatggcgcc
      421 ggccagattc atcacggtct gttcgtccaa ggaaaggtga tcccagcggc gcagcggcct
      481 ggacagcgcc gcgccgcgcc cgcccgctgc ccgctgccgg tatgtgtcag gcagacccgc
      541 gcgccgcgcg agcagcgtgc aggcagcccg gaagtccaga ccctccaggc gcatcacgaa
      601 gtggatgacg tcgccgtgtg ctcgacactg gccggcgaag caatgaaagt gttcgtcgcc
      661 ggcgtctcgc aggtcgaggc gcaggttcgg ccggcgacgg tcgttgtgga aggggcagag
      721 cgcccagaag cgtctttcgc cggccggcag caagcgcagg ccatagtcga gcgcaacctg
      781 gtcgagggga ttagcgcgtt tgagggccgc gacatcgtag tagtctccag gctggtatgc
      841 gtccgtgctc tgcgacgtct gcgcggcggt tgcgtcggct gtccggcgct ccagcgcatt
      901 catcgctgcc ttccttccgg ccgaaacgat acggaggtgc cgctgggcag tgcggcgctc
      961 gccgcttctg tgtgggcagc ggcctccagc gccgccacct cgcgcgggtc gctcgtggcg
     1021 aggtcgtgtt cctctgggca ggcgacgacc gtaagcgcga cccgactgcc acgcgcgaag
     1081 aagagtcctt cgcccttgcg cgcgccaagc aaatcgcgct cctcgcccga cgtcagcgca
     1141 aagagctggg cgaccgtgcg gaccgaggtc tcggtctgct tcaagagcag tttgacggcg
     1201 gtattcgcga ggatcgcggc gccatggtcg gtggccagaa aatcctcggc gcgatgcgtg
     1261 agcgcgacga cgccgagttc atacttgcgc gcccggcgat acatcgcttg caggaaatcg
     1321 gcgccggcgg ggtgctgcaa cagtttccac gcctcgtcca cgaccaggag gcgcggctgc
     1381 cgatcgcggc gaatgcggcc ccagatatag gcggtgatga tctggatcgc cagcggttgt
     1441 aagtcctttt cgagctgctg gatgctgaag acgacgagtc gtccatcgag gctgacattg
     1501 gtgcggcgat tgaccaggcc ggccagcgaa ccgctgacat accgttcgag gcgtaccacg
     1561 agagaggcgg ccaggtcgcc cggctgcgcc gtgagttcag cggcaaggtc accgaggagc
     1621 ggcgccggcc gcgaccagga ggttgggtcg gcgggcgtga tgccggaagc ggcgtaggtc
     1681 gcgtagagcg cccggtcgag cagcgcccgc tcggctgcgg ccagttgccg gcccggatcg
     1741 gccagcagca attccaggag ggtgagcacg ctcgccacct gctcggccac cgggtcgcgc
     1801 ggctcatcgt cgtcctgcgt gctgggtaac ggcaggtcga gcgggttgat cgcctggcgc
     1861 gaggcgagcg ccaggcggat gtactggccg ccgaccgccg cgcagagccg gcggtactcg
     1921 tcgtcggggt caatgatgaa cgagccaacg ccttccgcca gctgccgcag caccagcaac
     1981 ttcaggcaga aactcttccc cgcgcccgac gtggcgatca caaccatatt ggcgttggtg
     2041 aggctgcgat cgaagaggtc gaaaatcagc agcgactggt gggccagacc gaagagcact
     2101 ccggtacgca tcgccagggt gccggcgtcg aaggggaacg acgcggccag cgcggacgtc
     2161 gtgag
//
LOCUS       DASUBI010000258         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_490801, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000258 DASUBI010000000
VERSION     DASUBI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490801"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1667
                     /locus_tag="VFU72_04900"
     CDS             324..1667
                     /locus_tag="VFU72_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422857.1"
                     /translation="MHTTPPPTPPTPPTPDPGLAEIEAFLRTLIADLEPEPVSTGPGR
                     LRILPALALWGGFLVCVLRGFSSQLDLWRLLALGQLWDYPRYPVGDQAVYKRLAAAGT
                     QPLEQLFAQTSAVLADRLAPYAASDLAPFAREVVALDHSDLDPIARRLPRLRDLPPDD
                     AQLLPGQLAGLFDVRRQQWREIQFIPEPHQNEKVTARAMVAGLPPESLVLCDLGFFGF
                     AWFDDLTEQGLWYVSRLRQGTSYAVLHTYYADHETFDGLIWLGQHRADRAKHAVRLIR
                     FRHQGNLHHYITNVLDPLTCPLREIARLSARRWDFELAANLVKQHLGLHLVWSAKPVV
                     IQQQLWAVLLISQILQALRLEIAGRAGVDPFEVSMALLVRWLPRLAAAGQDPLQVFVA
                     QGRDVGFIRPSSRLTITAPTIPKEAIAPLPPDLPLERTPRHAHRKCGPRSAKETN"
     gene            complement(1731..2096)
                     /locus_tag="VFU72_04905"
     CDS             complement(1731..2096)
                     /locus_tag="VFU72_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422858.1"
                     /translation="MDNGASQPGRAVQTRSTPLQQEIANHLAALHELGPEYTDSVAAA
                     LVDELDQQIELKVRQVLAEGGVRQASTLHEKQKFVAMVLALGIPLLAIAGGIAGVFGI
                     VLVVAAIGVLGTVAMTHNF"
     gene            2242..2754
                     /locus_tag="VFU72_04910"
     CDS             2242..2754
                     /locus_tag="VFU72_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5422859.1"
                     /translation="MTLKPGWEGRFYEDFEVGDVYRSRYGRTITQADNIWFTLLSNNT
                     NPIHFDQHYAEQTEFGRPLVNSILTLAVVAGLGVADTSENGFALGWDEITLPNPLFEG
                     DTLYSESEVVAKRESRSKPEWGIVKFRSRGVQQDGTIVMEYTRSVMVWKRAHAPARDL
                     FPEPKESGNG"
     gene            2747..>3736
                     /locus_tag="VFU72_04915"
     CDS             2747..>3736
                     /locus_tag="VFU72_04915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5422860.1"
                     /translation="MAEPALSGITVLAFEQAAAGPFATLILADLGAEVIKVERPDSGD
                     VVRSWDRAVHGLSSGFVWLSRRKRSLTVDAKTPEGQAVLRRLAEQADVFLTNFAPGVA
                     ESLGLGYAELAERNPRLIYCALTGYGLSGPYRDAKAYDLLIQGEAGVLATTGYPDAPA
                     KVGLPISDIAAGMYAALGIVLALYQRDRTGRGQLVDVAMFDSILSWLGYFPHHYWHQG
                     EEAGRAGMRHHYIVPYGPYLARDGRYVNAVVASPRDWERFCRQVIERPELLEDPRFRD
                     SPARRENRAELEALVEAIFLERDSEEWFARLAAAELPHGALRGIAEVLAHPQVA"
BASE COUNT          567 a         1373 c         1205 g          591 t
ORIGIN      
        1 ggcccgcgcg ggctgacccc ggacgagctg gaggagttca gccaggccgg gctgcgacgg
       61 gtcgcgctcg aggagctgaa cgcaccggag agctccccct ggccgcagct ccgcgccgag
      121 taccagcgcg cctaacgcgc ggtcccggta ggggcagacc tccagatctg cccggtcgcc
      181 cctacgaccg ggcaatagga tgaggggaac gttggtggcg tcggtgttgt cgggcagcag
      241 ggcggcaagg acgagggcag acacggaggt catcgactcc aactaattcg cgtgggaggg
      301 gcgcactttg tcatactggt gggatgcata cgacaccacc acccacgcca cccacgccac
      361 ccacgccgga tcctggtctg gccgagatcg aagccttcct gcgcacgctg atcgccgatt
      421 tggagccgga gccggtcagc accggtcccg gtcgcctgcg catcctcccc gccctcgcgc
      481 tgtggggcgg cttcctggtc tgcgtgctgc gcggcttctc cagccaactc gacctctggc
      541 gcctgctcgc gctgggccag ctctgggact atccccgcta tcccgtcggc gatcaggcgg
      601 tctataagcg cctggcggcg gccggcaccc agcccttgga gcagctcttt gcccagacca
      661 gcgcggtcct ggccgaccgc ctggcgccct acgccgccag tgacctggcc cccttcgcgc
      721 gcgaggtcgt cgcgctcgac catagcgacc tcgatccgat cgcccggcgg ctcccccgct
      781 tacgggacct gccgccggac gatgcccaac tcctccccgg ccaactggcg ggcctgtttg
      841 atgtgcgccg ccagcagtgg cgggagatcc agtttatccc cgagccgcat cagaacgaga
      901 aggttaccgc ccgggccatg gtggccggct tgccccccga gagcctcgtg ctgtgcgatc
      961 tgggcttctt cggcttcgcg tggttcgatg acctgaccga gcaggggctg tggtacgtct
     1021 cccgcctgcg ccagggcacc agttacgcgg tcctgcacac ctactacgcc gaccacgaga
     1081 ccttcgatgg cctcatctgg ctgggccagc accgcgccga tcgcgccaag cacgccgtgc
     1141 gcctgatccg cttccgccac caagggaacc tgcaccacta catcaccaat gtgctcgatc
     1201 ccctcacctg ccccctgcgg gagatcgccc ggctctctgc ccgccgctgg gacttcgaac
     1261 tggcggccaa tttggtcaag caacacctgg gcttgcacct cgtatggtcg gccaagccgg
     1321 tcgtcatcca gcagcaactc tgggccgtgt tgctgatcag tcagattctg caagcgcttc
     1381 ggctggagat cgccgggcgg gcgggcgtcg atcccttcga ggtctccatg gcgctgttgg
     1441 tacgctggct gccacgcttg gctgcggccg gtcaggaccc gctccaggtc ttcgtcgcgc
     1501 agggtcgcga cgtcggcttc atccgcccct cgtcacggct gacgatcacc gcgccgacga
     1561 tccccaagga agccatcgct ccgctacccc cggacctgcc cttggagcga acgccgcgcc
     1621 atgcccaccg taaatgcggc ccccgttccg ccaaggagac gaattagttg gaggcgatga
     1681 cacaggggtc cgcccctaca ggcgtcaacg cgtgtgcacg acgccgaata tcagaagttg
     1741 tgcgtcatcg ccacggtgcc gaggaccccg atggccgcta ccaccaggac gatcccgaac
     1801 accccggcta tgccgccggc gatggccagc agcgggatgc ccagcgccag taccatggcc
     1861 acgaacttct gcttctcgtg cagtgtgctc gcctggcgca cgccgccctc ggccagcacc
     1921 tggcgcacct tcagctcgat ctgctgatcc aactcatcca ctagcgccgc cgccaccgag
     1981 tcggtgtact ccggccccag ctcgtgcagc gcggcgaggt ggttggcgat ctcctgctgc
     2041 agcggggttg accgcgtctg aaccgctcga cctggctggc ttgcgccgtt atccatgcca
     2101 tgctcctcgc ccgctgccgc gatcacctgc gctcccagtg gcgatagtat acccgctgac
     2161 ccgccggcat ccggcgcaac cgccgggcgc gtctctggta ttctctcccc gggtgatcgg
     2221 gcgcgcggag aggagccggc gatgacgctc aagccgggct gggaaggccg cttctacgag
     2281 gatttcgaag tgggcgacgt ctaccgctcg cgctacggac gcactatcac ccaggccgac
     2341 aacatctggt tcaccctcct ctcgaacaac accaacccga tccacttcga ccagcactac
     2401 gccgagcaga ccgaattcgg ccgcccgctg gtcaacagca tcctgacgct ggcggtcgtg
     2461 gccgggctgg gcgtggccga caccagcgag aacggcttcg cgctcggctg ggacgagatc
     2521 acgctcccca atcccctctt cgaaggcgat acgctctact cggagagcga ggtggtcgcc
     2581 aagcgcgagt cgcgctccaa gccggagtgg ggcatcgtca agttccgcag ccggggggtg
     2641 cagcaagacg ggacaatcgt catggagtac acgcgcagcg tgatggtctg gaagcgcgcg
     2701 cacgcgccgg cgcgcgacct cttccccgaa ccgaaggagt ccggtaatgg ctgagccggc
     2761 gctgagcgga atcacggtgc tggccttcga gcaggcggcc gccggccctt tcgccaccct
     2821 catcctggcc gacctgggcg ccgaggtgat caaggtcgag cggcccgaca gcggcgacgt
     2881 ggtgcgtagt tgggaccgcg cggtccacgg cctctcctcc ggcttcgtct ggctcagccg
     2941 ccgcaagcgc agcctcacgg tcgacgccaa gacgcccgag ggtcaggccg tgctgcggcg
     3001 gctggccgag caggccgacg tcttcctgac caacttcgcc cccggtgtgg ccgagagcct
     3061 ggggctgggc tacgccgagc tggccgagcg caacccgcgc ctgatctact gcgcgctgac
     3121 cggctacggg ctgagcggac cctaccgcga cgccaaggcc tacgacctct tgatccaggg
     3181 cgaggccggc gtgctggcca ccaccggcta ccccgacgcc ccggccaagg tcgggctgcc
     3241 gatctccgac atcgccgccg gcatgtacgc cgccctcggc atcgtgctgg ccctctacca
     3301 gcgcgaccgg actgggcgcg gccaactggt cgatgtggcc atgttcgact ccatcctctc
     3361 ctggctgggc tacttcccgc accactactg gcaccagggc gaggaggcgg gccgcgccgg
     3421 catgcgccac cactacatcg tcccctacgg gccgtatctg gcgcgcgacg ggcgctacgt
     3481 caacgcggtc gtcgccagcc cgcgcgactg ggagcgcttc tgccgccagg tcatcgagcg
     3541 gccggagctg ctggaggacc cgcgctttcg cgactcgccc gcccggcgcg aaaaccgcgc
     3601 ggaactggag gcgctggtcg aggcgatctt cctggagcgc gacagtgagg agtggttcgc
     3661 gcgcctggcg gcggccgagc tgccccacgg cgcgctgcgc ggcatcgccg aggtgctggc
     3721 ccatccccag gtagcc
//
LOCUS       DASUBI010000259         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_168301, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000259 DASUBI010000000
VERSION     DASUBI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168301"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFU72_04920"
     CDS             <1..289
                     /locus_tag="VFU72_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5422861.1"
                     /translation="RLAQTWNLRFSPHVWGGAVALAAALQLCAAVAPYPHVAREAAPI
                     YLEYDRGPNALREELAAAPFVVEGDRVRVPSGPGLGIELDWDAVARYRIDT"
     gene            complement(486..1541)
                     /locus_tag="VFU72_04925"
     CDS             complement(486..1541)
                     /locus_tag="VFU72_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEU5422862.1"
                     /translation="MPTTDLERATQSYAALKERFSQAPGLLRDAYPGNGAYAYLWPYS
                     QALQATLDMAALPNDGDEYREDVATRLDGLARYWRPEPQPPGYQSTVMPRLGQLSDVY
                     YDDNLWVALALVDAARQPGEEGWLARAAAIFDLSESGWDTTPRHPDPGGVFWVRADWN
                     HDRNTVSTAPTAKLALHLYQHGDDDRYLDWALRLLGWVERYLRAPDGLYWDHIDLQGK
                     IDKTEWSYNQGTMLGANALLYRATGRRVQLDRAREIASAALRRYGQQNFLGQPVAFNA
                     IFFRNLLLLGAIDGDEDYRHALRGYVDALWASAFDAATGLIRGEQGKSFSLLDHSAAV
                     QMAALLCWRPEQYAAIA"
     gene            complement(1717..2100)
                     /locus_tag="VFU72_04930"
     CDS             complement(1717..2100)
                     /locus_tag="VFU72_04930"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEU5422863.1"
                     /translation="MSAPLDAGSDPKPDERIGLMEGPEALPRKNGELVFQAPWEGRAF
                     GMAVALSDCHHYAWDDFRSRLIGQIAAAERAGVESSYYERWLAAFEELLIGRGILDRE
                     ELDARTAEYASGMHDDHHGDSHQHH"
     gene            complement(2113..2739)
                     /gene="nthA"
                     /locus_tag="VFU72_04935"
     CDS             complement(2113..2739)
                     /gene="nthA"
                     /locus_tag="VFU72_04935"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEU5422864.1"
                     /translation="MSEHHYGHDHGHDHHHEPVPVVARRAQALESLLIEKGLLSGEDI
                     DALVRSYENDIGPTRGAAVVARAWVDPAYKARLLADAPAALAELGIVHNEMVVVENTQ
                     DVHNVVVCTLCSCYPWSVLGLPPSWYKSAAYRSRVVIQPREVLREFGTELGEDIEVRV
                     WDSSAEMRYMVLPERPEHTEGMSEAELAELATRDAMIGVTKARQPATA"
     gene            complement(2780..3457)
                     /gene="nthB"
                     /locus_tag="VFU72_04940"
     CDS             complement(2780..3457)
                     /gene="nthB"
                     /locus_tag="VFU72_04940"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEU5422865.1"
                     /translation="MNGIHDMGGMHGFGPVDREGHEPGPETEWVRTVLALNRLLRGNG
                     YVNIDEFRYGIERMAPARYLTAPYFERWLETLVTNLIDKGVLTSDELAARAAGLDDAQ
                     TTGANQGVAPEAARKARPGVPATPSPRRETDRAPRFNIGDEVRTRNRHPTGHTRLPRY
                     ARGKRGVINRVQGVHVFPDTNALGLGEQPQHLYSVQFAGDELWGPDAEPGQYLYLDLF
                     EPYLEPA"
BASE COUNT          523 a         1263 c         1260 g          601 t
ORIGIN      
        1 acgcctggcc cagacctgga acctgcgctt ctcgccgcac gtctggggtg gcgccgtagc
       61 cctggcggcc gcgctgcagc tctgcgccgc cgtcgcgccc tacccgcacg tcgcccgcga
      121 agccgccccg atctacctgg agtacgaccg cggccccaac gccctgcgcg aggagctggc
      181 cgccgcgccc ttcgtggtcg aaggcgaccg cgtgcgcgtc cccagcggcc cgggtttggg
      241 gatcgagctg gactgggacg cggtggcgcg gtacaggatc gatacttgag tggcactgcc
      301 cagggacgac gttgagtgcc atcgaggacg gatccttcga caagctcagg atgacaggga
      361 taggcagcag cctagcggaa gagggaggcc gtcgtcgttc gcctcatccc tagtcgtgtc
      421 atcctgagcg cagtcgaagg atccgtcctc gcaggccatc attccatgtc ggtaggcgcc
      481 gcgccctacg cgatcgccgc gtactgctcc ggccgccagc agaggagcgc ggccatctgg
      541 accgcggcgg aatggtccag cagtgagaag gacttgccct gctcgccacg gatgaggccg
      601 gtcgcggcgt cgaaggcgct cgcccagagg gcgtcgacat agccgcgcag cgcgtgacgg
      661 tagtcctcat caccgtcgat cgcccccagg agcagcaggt tgcggaagaa gatggcgttg
      721 aaggccaccg gctgacccag gaaattctgc tggccgtagc gccgcagcgc cgcgctggcg
      781 atctcgcggg cgcggtcgag ttgcacccgg cgtccggtgg cgcggtagag gagggcgttg
      841 gcgccgagca tcgtgccctg gttgtagctc cactcggttt tgtcgatctt gccctgcagg
      901 tcgatgtggt cccagtagag cccatcggga gcgcgcagat agcgctcgac ccaacccagg
      961 agccgcagcg cccagtcgag gtaccgatcg tcgtcgccgt gctggtagag gtggagcgcc
     1021 agcttggcgg tgggcgcggt cgagacggtg ttgcggtcgt gattccagtc cgcgcgcacc
     1081 cagaagacgc cgccgggatc ggggtgcctg ggagtggtgt cccaaccgga ctcgctcagg
     1141 tcgaagatcg cggcggcccg cgccagccag ccttcctcgc caggctgccg cgccgcgtcg
     1201 accaacgcca gcgccaccca gaggttgtcg tcgtagtaga cgtcgctgag ctggcccagc
     1261 cgtggcatca ccgtcgactg gtagccgggc ggctggggct ccggccgcca gtagcgcgcc
     1321 agcccatcca ggcgcgtggc gacatcctct cggtattcgt ccccgtcatt cggcagcgcg
     1381 gccatatcga gcgtcgcctg cagcgcctgc gagtagggcc agagataggc gtaagcgcca
     1441 ttgccgggat aggcgtcgcg tagcagcccc ggcgcttgac tgaagcgctc cttcagcgcg
     1501 gcatagctct gcgtggcacg ctccagatca gtggtcggca tggtcggctc ctcgggttcg
     1561 acgacgaacg cgcacgagac cgcgctcgtc ctgccgcccg cagcctccct gcccgcacct
     1621 cggcgcggag gccgaattga tgcagcgagc cggccatgtg gccggctcgc tacggcctga
     1681 ggcggactgg agccgctaga caggacgtgt ggaatctcag tgatgctgat gcgaatcgcc
     1741 gtggtggtcg tcgtgcatcc cggaggcgta ctcggcggtg cgcgcgtcga gctcctcgcg
     1801 atcgagaatg ccccgcccga tgagcagctc ctcgaaggcg gccagccagc gctcgtagta
     1861 gctcgactcc accccggcgc gctcggcggc ggcgatctgc ccgatcaagc gtgagcgaaa
     1921 gtcgtcccag gcgtagtggt gacagtcgct cagggccacg gccatgccga aggcgcgccc
     1981 ttcccagggc gcctggaaga ccagctcgcc gttcttgcgc ggcagcgcct cagggccctc
     2041 cattagaccg atacgctcgt ccggcttcgg gtcggagccg gcgtccaatg gtgcgctcat
     2101 ctcgctccct ccctaggccg tcgccggttg cctggccttc gtcacgccga tcatcgcgtc
     2161 gcgcgtagcc agctccgcca gctcggcctc actcatgccc tcggtgtgct cgggccgctc
     2221 gggtaggacc atgtagcgca tctcggcgct gctgtcccag acgcgcacct cgatgtcctc
     2281 gcccagttcc gtgccgaact cgcgcagcac ctcgcgcggt tggatgacca cgcgcgagcg
     2341 gtaggcggcg ctcttgtacc agctcggcgg caggccgagc accgaccagg ggtagcacga
     2401 gcagagcgtg cagacgacga cgttgtgaac gtcctgggtg ttctcgacca cgaccatctc
     2461 gttgtgcacg atgccgagct cggccagcgc cgcgggggcg tcggccagca ggcgcgcctt
     2521 gtacgcggga tcgacccagg cacgcgccac gaccgcggcg cctctggtcg ggccgatgtc
     2581 gttctcatac gagcgcacca gggcgtcgat gtcctcgccg gagagcagcc ccttctcgat
     2641 cagcagcgac tccaacgcct gcgcgcgccg cgccacgacc ggtaccggct cgtggtgatg
     2701 gtcgtgaccg tggtcgtgtc cgtagtgatg ctcgctcacg tctcgctcct ttccaagcgg
     2761 gtgtgccttc ggcgcctgct cacgccggtt ccaggtaggg ctcaaagagg tcgaggtaga
     2821 gatactggcc cggctcggcg tccggacccc agagttcgtc gccggcgaac tggacgctgt
     2881 agaggtgctg gggctgctcg cccaacccga gcgcgttggt gtcgggaaag acgtggaccc
     2941 cctgtacccg attgatcacc ccgcgcttgc cgcgggcgta gcgcggcagc cgcgtgtggc
     3001 cggtcgggtg gcggttgcgg gtgcgcacct cgtcgccgat gttgaagcgg ggcgcgcgat
     3061 cggtctcacg ccgcggcgat ggcgtcgccg ggacgcctgg ccgtgccttc cgcgcagctt
     3121 caggcgccac tccttggttc gcgcccgtcg tctgggcatc gtccagcccg gccgctcgcg
     3181 ccgccagttc gtcggacgtc agcacgccct tgtcgataag gttggtcacg agcgtctcca
     3241 gccagcgctc gaagtagggc gcggtcaggt aacgcgccgg tgccatgcgc tcgatgccgt
     3301 agcggaattc gtcgatgttg acgtagccgt tcccgcgcaa gaggcggttc agcgccagca
     3361 cggtccgaac ccactcggtt tcgggtcccg gttcgtgccc ctcgcggtcg accggtccga
     3421 agccgtgcat accgcccatg tcgtggatgc cgttcatccc tccctcctct ctaatcgccg
     3481 gccctcgcgg ccggcagtga attgccgggg atcgcgaaaa tcggacggta ggcgtcatgg
     3541 tcggtgccgt agtagtcggc cagcagcgcg gcgtaggcgg cggcctgtgc gggccgcgtg
     3601 cgccagcgca cgcccaggta ctcggccgcc ttgaccacac ccagcac
//
LOCUS       DASUBI010000260         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_261316, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000260 DASUBI010000000
VERSION     DASUBI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261316"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFU72_04945"
     CDS             <1..321
                     /locus_tag="VFU72_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422866.1"
                     /translation="TAEERTPPSFRAPAGALEDSYYLGTGRQLWDGSLLRCSLLISRG
                     GRDFWSAPGDPERLAAQAVHAAEVRRLEQPEATHFVHLDRPERGRAALLDAVRDFLEP
                     APPS"
     gene            complement(392..1204)
                     /locus_tag="VFU72_04950"
     CDS             complement(392..1204)
                     /locus_tag="VFU72_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HEU5422867.1"
                     /translation="MAQNQSAHPFDVALPVMYVAPGQSLTGAYISADSIEHLAALGQA
                     ASSLGVTLSLVYTYWDPSQRRYVGMVVLDQTNTQGGAQRVTQALGQAPGVQVLATAGA
                     GPGSGLVAFEQNRLSVAGTPVVVIARPFLGGTHRRLLEALGDQAATLLFQSGQSAGQM
                     AASGVPDLVKHLGVPLSPDLVRQRFRDLQVFSWATVVDLKVNDRFAGEARLADDFEAA
                     IWQGQAQSAVCHWIRGFLTGALSSLTGHAIQVSEPECQAKGDQYCRMTFQAS"
     gene            complement(1269..1652)
                     /locus_tag="VFU72_04955"
     CDS             complement(1269..1652)
                     /locus_tag="VFU72_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5422868.1"
                     /translation="MSRDTVEAAIQRYYAAINARQPAAIAGVFAPDGVFHYPGRPPLH
                     GRAAIEAAEQQILAPINTITLTPEETYIDGPRAAVKFGMRVRTRDGGSASMEGIDTFV
                     VNPDGTLQQVDVYYDARALVALTTS"
     gene            1823..2050
                     /locus_tag="VFU72_04960"
     CDS             1823..2050
                     /locus_tag="VFU72_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422869.1"
                     /translation="MPGFAKDIQPLFTQEDIEHMLGVDPDLNLGSYDSVKKRVNDIYR
                     MVSFGAMPPGHPWPKSQGDLFKRWMDAGSPR"
     gene            complement(2069..3205)
                     /gene="tal"
                     /locus_tag="VFU72_04965"
     CDS             complement(2069..3205)
                     /gene="tal"
                     /locus_tag="VFU72_04965"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5422870.1"
                     /translation="MMTDNPLRELEQCGQSVWLDAISRDLLRSGQFAHLIKQDGVSGV
                     TANPSIFEKAIVGTHDYDESIRELARAGNAPLQIYEQLAIHDVGAAADLLRPTYERTQ
                     GRDGFASIEVSPDLAAKTEASIEEARRFWRELNRPNVMIKIPATAEGIPAIEALTAEG
                     INVNITLIFAVEMYERVMEAYLRGLERRVEANQSITHIASVASFFVSRVDTLVDTLLR
                     KRIDAAPDQRAALEAFAGTIAIANAKIAYQRFQTVFASQRFTALQAKGAQLQRPLWAS
                     TSTKNPAYGDTRYVASLIGPHTVNTMPLETIDAFRDHGAVDCGAVTEGLAGAQQMMSA
                     LERAGISMTTVTDELIREGVDKFCQALHSLLETIGARQGKLVSP"
     gene            complement(3890..>4296)
                     /locus_tag="VFU72_04970"
     CDS             complement(3890..>4296)
                     /locus_tag="VFU72_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422871.1"
                     /translation="RLDDRARQVLPASIPTAAFADGAELRGSTLEPHGRSGQIAVLLT
                     WRLPGDGHGRRARLLLRPTQPGMPDESATVDLPGFTQPTDVVRLVILDAPPDGAEADL
                     FVSLLDAAGRPVPGSGVDTDGRLFLNRYRFTR"
BASE COUNT          612 a         1455 c         1553 g          676 t
ORIGIN      
        1 accgccgagg agcgcacccc gcccagcttc cgcgcgccgg ccggagccct ggaggatagc
       61 tactacctcg gcacgggccg gcaactgtgg gacggctcgc tgctgcgctg cagcctgctg
      121 atctcgcgcg gcgggcgcga cttctggtcg gcgcccggcg acccggagcg gctggcggcg
      181 caggcggtcc acgccgccga ggtacggcgg ctggagcagc cggaggccac ccacttcgtc
      241 cacctcgatc gccccgagcg cgggcgcgcc gccttgctcg acgccgtgcg cgatttcctg
      301 gagccggccc cgccgtcgta gcccggggtt gcgatcatca accagcgatc ttgtcgtccg
      361 ccggcgggaa gccgaaggaa cgccgcgtcc ctcaggacgc ctggaacgtc atgcggcagt
      421 actggtcgcc cttggcctgg cactcgggct cgctgacctg gatcgcgtgc ccggtcaggg
      481 aggagagcgc gccggtcagg aagccgcgga tccagtggca aacggcgctc tgggcctgcc
      541 cctgccagat cgccgcctcg aaatcgtcgg ccagccgcgc ttcgccggcg aagcggtcgt
      601 tgaccttgag gtcgacgacg gtggcccaac tgaagacctg taagtcgcgg aagcgctggc
      661 gaaccaggtc gggcgagagc ggcacgccca ggtgcttgac caggtcaggc accccggagg
      721 ccgccatctg cccggcgctc tgacccgact ggaagaggag ggtggccgct tggtcgccca
      781 gcgcctccag cagccggcga tgggttccgc cgaggaaggg ccgggcgatc accaccaccg
      841 gggtgccggc gacgctcagc cggttctgct cgaaggcaac caggccggag ccgggaccgg
      901 ctcccgccgt ggccagcacc tgcacccccg gcgcctggcc cagcgcctgg gtgacgcgct
      961 gcgccccgcc ctgcgtgttg gtctggtcga gcacgaccat gccgacgtag cgtcgctggc
     1021 tggggtccca gtaggtgtag accagggaga gcgtcacgcc cagggagctg gccgcctgcc
     1081 cgagcgccgc caggtgttcg atgctgtcgg cgctgatgta ggcgccggtg agcgactgcc
     1141 ccggcgccac gtacatgacg ggcagggcca cgtcgaaagg atgtgcggat tgattctgcg
     1201 ccataccgcg gtccttcctg catgatccag gcgatgcgtg gatcgattct gctgccccgg
     1261 cgccgcgctc aggacgtcgt cagggccacc agggcgcggg cgtcgtagta gacatcgacc
     1321 tgctggagtg tcccgtcggg gttgaccacg aaggtgtcga tgccctccat gctggcggag
     1381 cccccgtccc gggtgcgtac ccgcatgccg aacttcacgg cggcgcgcgg gccgtcgatg
     1441 taggtctcct ccggcgtcag cgtgatcgta ttgatggggg ccaggatttg ctgctcggcg
     1501 gcctcgatcg ccgcgcggcc gtgcagcggc ggccgccccg ggtagtggaa gacgccgtcc
     1561 ggcgcgaaca ccccggcgat ggccgccggc tgccgggcgt tgatcgccgc gtagtagcgc
     1621 tggatggccg cctccaccgt gtcacgcgac acctcccatc ctgctgtgtc gtggttgcga
     1681 gtgccgagcg gagttgcagc agaagccggg ccagcggcgc atgccacggc gcatggagcc
     1741 gccgtggctt ggtcgatgcg ggcacgatcg tgtccatccg tgacggcccg ttccatcggg
     1801 tcgctcgtgc gaggagtcag acatgcccgg cttcgcgaag gacatccagc cgctctttac
     1861 ccaggaggac atcgagcaca tgctcggcgt ggatcccgat ctcaacctcg gcagctatga
     1921 ctcggtaaag aagcgcgtca acgacatcta ccgcatggtg tcgttcggcg cgatgccgcc
     1981 cggccacccc tggcccaaga gccaggggga cctcttcaag cgatggatgg acgcgggctc
     2041 gccgcggtga gtgtccgcgc cggccgcgct aggggctgac cagcttcccc tggcgcgcgc
     2101 cgatcgtctc tagcagcgag tgcagcgcct ggcagaactt gtctacgccc tcgcggatca
     2161 gctcgtcggt gaccgtcgtc atgctgatgc ccgcgcgctc cagcgcgctc atcatctgct
     2221 gtgccccggc caacccctcg gtcaccgccc cacaatcgac cgcgccgtga tctcggaagg
     2281 cgtcgatcgt ctcaagcggc atggtgttga cggtgtgcgg gccgatcagg ctggcgacgt
     2341 agcgcgtgtc gccgtaggcc gggttcttgg tactggtgct ggcccagagc gggcgctgga
     2401 gctgcgcgcc tttggcctgc aaggccgtga agcgctgtga ggcgaagacg gtctggaagc
     2461 gctggtaggc gattttggcg ttggcgatgg cgatggtgcc cgcgaacgct tccagagcgg
     2521 cgcgttggtc cggcgccgcg tcgatccgct tccgcagcag cgtgtcgacc agcgtatcca
     2581 cccggctgac gaagaaggag gcgaccgagg cgatgtgcgt gattgactgg ttcgcctcga
     2641 ctcgccgctc cagcccgcgc aggtaggcct ccatgacgcg ctcgtacatc tcgaccgcga
     2701 agatcagcgt gatgttgacg ttgatgccct ccgcggtcag cgcctcgatc gccgggatac
     2761 cctcggccgt cgccgggatc ttgatcatca cgttcggccg gttcagctcg cgccagaagc
     2821 ggcgggcctc ctcgatgctg gcctcggtct tggcggcgag atccggcgag acctcgatgc
     2881 tggcgaagcc gtcgcggccc tgggtgcgct cgtaggtcgg acgcagcagg tcggcggccg
     2941 cgccgacgtc gtggatggcg agctgctcgt agatctgaag gggcgcattc ccggcgcgcg
     3001 ccagttcgcg gatgctctcg tcgtagtcgt gggtgccgac gatggccttc tcgaagatgc
     3061 ttgggttggc ggttacgccg ctgacgccgt cctgcttgat caggtgggcg aattggccgg
     3121 agcgcagcag atcgcggctg atcgcgtcga gccagacact ctggccgcac tgttcgagct
     3181 cccgcagcgg gttgtcagtc atcatgtcgc ctccgtctga tagcgctgat cccagtgttc
     3241 tcgaccgatg agacccgccg ccccgcgctt cgagcaccgc tcaatccggt cgctgatcgg
     3301 cgaatcacca ggcatccagc aagtcccgga ccaacccgag gagccgtgcg accgccgcgg
     3361 ccgtgtcccc gggcgatcac gcactgttcc cccttcgtcg tgcgcccgcc gcagggcaag
     3421 ggcgcggccg caactcctct cttctagcgg tgccacggtt gtcgccattc ctgaaaagga
     3481 gagatccttc acccccgacc ggtcattctg aggccacggc cgaagaatct ctcgtcaccg
     3541 ccgagcgagg gtcacgccgc acgtgaggag cggtcctttc cccctcggtc atgctgaggc
     3601 cgcagccgaa gaatctcctc tcattcgcag cagcactgcc gcttgttgct gacgggagat
     3661 ccttcgcggc ggctcaggct gacagggcag cgcccaggat gacagggggg aagggcgcgg
     3721 gctgaccgag cagcggcgcc gcacgacagc ggtggcggct caggctgaca ggagggagag
     3781 ggcgcgggct cacacgggcg actcaggcta accggagggg cggaggccgc gtcgatgctg
     3841 ggcttcgtgc gccggtatcg ggggcggcgc gacagccgcc gcgagcgcgc tagcgcgtga
     3901 agcggtagcg gttgaggaag agccggccgt cggtgtcgac gccgctgccc gggaccggcc
     3961 ggccggcggc gtcgagcagg ctgacgaaga ggtcggcctc ggcgccgtcc gggggcgcgt
     4021 cgaggatgac cagccgcacc acgtcggttg gctgggtgaa gccggggaga tcgacggtgg
     4081 cgctctcgtc gggcatccct ggctgcgtcg ggcgcaggag caggcgcgcc cggcggccgt
     4141 gaccgtcgcc cggcagacgc caggtcagca ggacggcgat ctggccgctc cggccgtgcg
     4201 gctccagggt gctgccgcgc agctcggcgc cgtcggcgaa ggcggcggtg gggatgctgg
     4261 ccggtaatac ctggcgggcg cggtcgtcga ggcgaa
//
LOCUS       DASUBI010000261         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_890500, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000261 DASUBI010000000
VERSION     DASUBI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890500"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFU72_04975"
     CDS             complement(<1..585)
                     /locus_tag="VFU72_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEU5422872.1"
                     /translation="MSSRLPSHEGKLPFRGHEVWYRVVGEGEEPGKLPLLTLHGGPGA
                     AHDYLEPLEGLAASGRRVIFYDQLGCGRSDQPHDPAMWTVELFLEEIEAVRAALGLDE
                     LHLLGQSWGGMLGMEYALTQPPGLASLTVASSPASMCQWVEEANRLRAELPPETQATL
                     LRHEAAGTTDSPEYQQAMAVFYGRHVCRLDPMPDF"
     gene            1413..1628
                     /locus_tag="VFU72_04980"
     CDS             1413..1628
                     /locus_tag="VFU72_04980"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEU5422873.1"
                     /translation="MKIEFDKLVNILYLRLRPGTVERTIEFAQDVYLDIDDEGHVLGA
                     EFVNAGDFFAILNQHDGKLDIPERIPA"
     gene            1787..3151
                     /locus_tag="VFU72_04985"
     CDS             1787..3151
                     /locus_tag="VFU72_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5422874.1"
                     /translation="MAELDDVYRYIDDHRDEYIALVQRLCRQPSISAQNVGIQETAAL
                     VKEVLENAGASARIVPIEGGNPVVYAEAGPAGKAKTLSFYNHYDVQPPEPLDLWEVDP
                     FAAEIRDGRIYARGVADNKGNIASRVAAIDAYRTVRGALPLRVKFIIEGEEEIGSPHL
                     ERFTAEHPDLVQADGNIWESGSKDVEGRPIISLGLKGICYVELRVRSTARDLHSSLGA
                     SVPNAAWRLTWALASLKGPDERVRIPGFYDKVVPPTEDDLKALDSLPDTEAKRRELYQ
                     IDQFVTGLSGRPLQLKEYFQPTCTICGLESGYTGPGAKTVMPAQAMAKVDFRLVIDQD
                     PEEIRGLLRRYLDDQGFTDIEIIPFSMEHPARTSPDAEIAQVATAAHRELDGVEPIVV
                     PTSLGTGPAYVLAYQFGIPFASSGVGHADSRAHSPMENIYVEDYVLGIKRAVRIMDGF
                     ARAR"
     gene            3316..>4027
                     /locus_tag="VFU72_04990"
     CDS             3316..>4027
                     /locus_tag="VFU72_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422875.1"
                     /translation="MEKNRRWAATASLVVTLVLLLLIYVGSRGLRDFDAALIGYAVAT
                     VFMAAALTYRYALWVGRPPTWRYFRAGWVEFLSWKNFRRYTLLIPRAWWTDIFGQTFI
                     KRRSTTRWVMHLCIFWGVIISLLVTIPLTFGWIHFTLVPPSHYRAWFFGIPVLTFPIE
                     AGTGFAVFHILDFSAVLLIVGLTIAIWRRLTNAGLLTTQRFGFDIMPLVLLFAIAITG
                     LALTASYTWWQGRFYWFIS"
BASE COUNT          654 a         1428 c         1255 g          690 t
ORIGIN      
        1 gaaatcgggc atcgggtcga gccggcagac gtgccgccca tagaagaccg ccatcgcctg
       61 ctggtattcg ggcgagtcgg tcgtgccggc cgcttcgtga cgcagcagcg tcgcctgcgt
      121 ctcgggcggt agctccgcgc gcagccggtt ggcttcttca acccactggc acatcgaggc
      181 gggcgaactg gcgaccgtca ggctggccag gccgggcggc tgggtcaggg cgtactccat
      241 ccccagcatg ccgccccacg attgcccgag cagatgcagc tcgtccagcc ccagcgcggc
      301 gcgcacggcc tcgatctcct ccaggaagag ctcgaccgtc cacatggcgg ggtcgtgcgg
      361 ctggtcggag cggccgcaac cgagctgatc gtagaagatg acgcgccggc cgctggcggc
      421 cagcccctcc agcggctcca ggtagtcgtg ggccgcgccc ggcccgccgt gcagcgtcaa
      481 gagcggcagc ttgcccggct cctcgccttc gccgaccacg cggtaccaga cctcgtgccc
      541 gcgaaacgga agctttccct catggcttgg tagccggctg ctcatcgcga cctcctccgc
      601 ccctgtgcct gtccaccctt gatgccgcct gatgcgcggg accgtatgga acggatgcca
      661 caatccgtgg agcttcggaa ccacgcggcg cgcggggtta aaaccaccgc gctaaatcca
      721 acgaagccca ctaaaggggc tgggtcttgg atcggttgtg atcgaggcat cctcgaccgc
      781 gaccagcttt gtccacagtc atccgaaaag gaccgtcgcc ttcgcccgcc gcaagggtgg
      841 agaggcgcat tgccaaatct cagccccttc agtgggcttc gtaaagatta gcgcggtggt
      901 tttaaccccg cgcgccggcg ggcacgcatg tcccagattc gttgctcaat gatcattgac
      961 cactgattac ccccgcgcgt cgcgggcgct aaggctcgta gatggccgct catccgcact
     1021 cataccggcg actctagccc gccggcgcgc agccgtcaac gcgctggcga agatggctga
     1081 gatcgcccat catcgatcgt cgctcgctgt gggccgtgta tgatgtgtcc gtcgatgtca
     1141 acctcggagg ctccgtgtgg actccggatc aggtggaaat cccccgacta tgcccgtaat
     1201 cgtatgcacg aacggaggac ctccgagcgg caagtactca acgcactcaa cgcactcaac
     1261 gatccacatc ggcattatcc cagccgtgac ggcaggaccg ttgcagagcg tgacacgggc
     1321 atggggaacg ttctccgtgt cgtctatctc gatcgcccgg aaggaccgct catcgtgacg
     1381 gtggtccgca tctcgccgaa aggcggtaca aggtgaagat cgaattcgac aagttggtga
     1441 atatcctcta cctccgtctg cgcccgggca ctgtcgagcg gacgattgag tttgcccagg
     1501 atgtctatct tgatatcgac gacgagggcc acgtgctcgg cgccgagttc gtcaacgccg
     1561 gcgacttctt tgccatcctc aaccagcatg acggcaagct cgacatcccc gagcgtatcc
     1621 cggcctagct ctacccgcgg caatccgccc ggcgatcgcg ccggattctt ctttgcccgc
     1681 cgcgacttcg atcatgctct ggttgacagc cgcgcggtca gcgccgagag tgtgcgcgcg
     1741 gcccacgcgg ggccgctgga tacacgggga gtgaggagcg ctggtcatgg ccgagttgga
     1801 cgacgtctat cgctatatcg acgaccaccg cgacgaatac atcgcgctgg tgcagcggct
     1861 ctgccgccag ccaagcatct cggcgcagaa cgtcggcatc caggagaccg ccgcgctggt
     1921 caaggaggtg ctggagaacg ccggggccag cgcccgcatc gtgccgatcg agggcggcaa
     1981 cccggtggtc tacgccgagg ccgggccggc gggcaaagcg aagaccctgt cgttctacaa
     2041 ccactacgac gtgcagccgc cggagccgct cgatctttgg gaggtcgacc ccttcgccgc
     2101 cgagatccgc gacggtcgca tctacgcccg cggcgtggcc gacaacaagg ggaacatcgc
     2161 ctcacgcgtg gccgccatcg acgcctaccg cacggtgcgc ggcgcgctgc cgctgcgagt
     2221 caagttcatc atcgagggtg aggaggagat cggcagcccg cacctggagc gcttcacggc
     2281 cgagcatccc gatctggtgc aggccgacgg caacatctgg gagagcggca gcaaggacgt
     2341 cgaggggcgc ccgatcatct cgctcggtct caagggcatc tgctacgtcg agctgcgcgt
     2401 gcgctccacc gcgcgcgacc tgcattcctc cctcggagcc tcggtgccca acgccgcctg
     2461 gcgcctgacc tgggcgctgg cctcgctcaa ggggcccgac gagcgcgtgc gcatccccgg
     2521 cttctacgac aaggtcgtgc cgccgaccga ggacgacctc aaggcgctcg actcgctgcc
     2581 cgacaccgag gccaagcgcc gggagctcta ccagatcgac cagttcgtca ccgggctcag
     2641 tggccgcccg ctgcagctca aagagtactt ccagccaacc tgcaccatct gcgggctgga
     2701 gtcgggctac accggccccg gcgccaagac ggtcatgccg gcccaggcga tggccaaggt
     2761 cgacttccgc ctggtgatcg accaggatcc ggaggagatt cgcggcctgc tacgcagata
     2821 cctggacgac cagggcttca cggacatcga gatcatcccc ttcagcatgg agcacccggc
     2881 gcgcacctcg cccgacgccg agatcgcgca ggtggccacc gcggcgcacc gcgagctgga
     2941 cggcgtggag ccgatcgtgg tgccgaccag cctgggcacc ggcccggcct acgtgctggc
     3001 ctaccagttc ggcatcccct tcgcctcctc aggcgtcggc cacgccgact cgcgggcgca
     3061 ctcgccgatg gagaacatct atgtcgagga ttacgtcctc ggcatcaagc gcgccgtccg
     3121 catcatggat ggcttcgccc gcgcccgtta ggacgcatcc cgagccgctg tcaaacctac
     3181 cccgacagca catcctgctc ccctctccca gaattgggag aggggttggg ggtgagggcg
     3241 cgtcgggcat caaccgtgcg agacaatcgc cctgtatgat gcgctggggc cgggcctagg
     3301 gggcaggagg cgtctgtgga gaagaaccgg cggtgggccg ccaccgcctc gctggtggtc
     3361 acgctcgtcc tgctgctgct gatctacgtc ggctcgcgcg ggctgcgcga cttcgacgcc
     3421 gccctgatcg gctacgccgt ggccaccgtc ttcatggcgg ccgccctcac ctaccgctac
     3481 gctctctggg tcgggcgccc gcccacctgg cgctacttcc gcgccggctg ggtcgagttc
     3541 ctctcctgga agaacttccg ccgctacacc ctgctcatcc cgcgcgcctg gtggaccgac
     3601 atcttcggcc agaccttcat caagcgccgc tcgaccaccc gctgggtcat gcacctgtgc
     3661 atcttctggg gggtgatcat ctcactcctg gtcactatcc cgctgacctt cggctggatc
     3721 cacttcacgt tggtcccgcc cagccactac cgcgcctggt tcttcggcat cccggtgctc
     3781 accttcccga tcgaggccgg caccggcttc gccgtcttcc atatcctcga cttctccgcc
     3841 gtgctgctga tcgtcgggct caccatcgcc atctggcgcc ggctgaccaa cgccgggctg
     3901 ctgactaccc agcgcttcgg cttcgacatc atgccgctgg tcctcctctt cgccatcgcg
     3961 atcaccgggc tggcgctgac ggcctcttac acctggtggc aggggcgctt ctactggttc
     4021 atctcgc
//
LOCUS       DASUBI010000262         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_166686, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000262 DASUBI010000000
VERSION     DASUBI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166686"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFU72_04995"
     CDS             complement(<1..390)
                     /locus_tag="VFU72_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422876.1"
                     /translation="MPRRRILLASAIALLVLLALPLSGALAYGKQDQPLAQLAFSGNC
                     DNPDYPLCAPPPAGFGTGGIWLWIEVDANGTGDVAGAGCGHTVGGVGGPGGAGAGSIR
                     GEITWQYSSGANLPANAFVVGVDPTDTY"
     gene            857..>2469
                     /locus_tag="VFU72_05000"
     CDS             857..>2469
                     /locus_tag="VFU72_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5422877.1"
                     /translation="MPTTWQESDVERPILDDISLAEPWALLERFSSLVRLSGSPEEAE
                     AVAYITGKLSQWGVPHRVHHPVTLISLPGPASLRTLGPQERSFTVKTSSFSPSTNGEE
                     ITGELVYVPGRQAQAIGDLFSGAPTVVGRQDLRGKVVMTEGLGIAARGLDLAGSGAIA
                     ALFINPGERIHEGITTTTWGSPDLGSQGRTPPVPVFGINHPEGEALKALLAEGPVRVA
                     LANQVDTGWRPIPVIVAEIAGSQAADEFVLFHGHLDSWHVGIGDNATGDATLLEVARV
                     FYQHREQLARTLRVAWWSGHSHGRYAGSTWYAQEFAQDLVANCVAHVNCDSPGCRWAT
                     EYRDVYAMAEAAGLVEAAVRDVTGQAASTEPPLRAGDISFNNLGISTCFMLSSTMPLE
                     LAREKGYYGVGGCGGNIAWHTEDDTMEIADRDNLLRDLRVYGATLLRLLNAPLYPLDY
                     RATVDQIAAIVERLQQAAGEKFDFTASLRAAQDLRATLDRFYGEAVELSDREADDPAV
                     RQANASQRLLARSLVTTVYSQDGRFRQDPA"
BASE COUNT          389 a          900 c          829 g          351 t
ORIGIN      
        1 gtaggtatcg gttggatcga caccgacgac gaaggcattg gccggcagat tcgcgcccga
       61 ggagtactgc caggtgatct caccgcggat cgagccggcg ccggcgccgc cgggcccgcc
      121 gacgccgccg accgtgtggc cgcagcccgc accggcgaca tcgccggtcc cgtttgcgtc
      181 aacctcgatc cacagccaga tgcctccggt tccaaatccc gccggaggcg gcgcacacaa
      241 cggatagtct gggttgtcgc agttgccgga gaatgcaagc tgggccaggg gctggtcttg
      301 cttgccgtag gccagcgctc cgctcagcgg cagagccagg aggacgagca gcgcgatggc
      361 cgaagcgagc aggatacgtc gacgaggcat ggcgtctctc tccctctcct ctcccatcac
      421 cgtcttacga cgatggggaa aatgaacggc caccgtcggc gtcccaggca agcgacccac
      481 cggcgatgac ctccgcgcgc aatcttccgc tcctctgagt tgtcgagttc gtccatgata
      541 gcggcaagcg tcacggttgt cactatccgt gctgggccag tttcattggc gacgcgatcc
      601 gttcggtagg ggcccgcgcg gactctcggg cagcaccgag cagccggcca ccgtcgggct
      661 cggtattccg gatgcacccg cgaggatcgc tgggctgatg agactgccag caggccggcg
      721 acgcgaatgc ccggcggcct gcaagtggca gagcgtccgt cgcacgatgc ggcgccagag
      781 ccgatgcgct accattgcgg ccactgaatg cagccgagcc gccgccgagc gcgccccgca
      841 cacgaaggag tcacgcgtgc caaccacctg gcaggagtcc gatgtcgagc ggccaatcct
      901 cgacgacatc tcgctggccg agccctgggc gctcctggag cgcttcagca gcctagtccg
      961 gctctccggc agtccggagg aggccgaggc ggtggcctac atcaccggca aactctcgca
     1021 gtggggcgtg ccccaccggg tgcaccatcc ggtgacgttg atcagcctgc ccggcccggc
     1081 cagcctgcgc accctcggcc cgcaggagcg ctcgttcacg gtcaagacct cctccttctc
     1141 cccctcgacc aacggtgagg agatcacggg cgagctggtc tacgtgccgg gccgccaggc
     1201 acaggccatc ggcgacctct tcagcggcgc gcccaccgtc gtgggccggc aggacctgcg
     1261 cggcaaggtg gtgatgaccg aggggctggg catcgccgcg cgcgggctcg atctggccgg
     1321 ctccggcgcc atcgccgcgc tcttcatcaa ccccggcgag cgcatccacg aggggatcac
     1381 gaccaccacc tggggctcgc ccgacctcgg ctcgcagggc cgcacaccgc cggtgccggt
     1441 cttcggcatc aatcaccccg agggcgaggc gctcaaggcg ctgctggcgg aggggccggt
     1501 gcgggtggcg ctggccaacc aggttgacac cggctggcgc ccgatcccgg tgatcgtggc
     1561 cgagatcgcc ggcagccagg ccgccgacga attcgtcctc ttccacggcc acctcgactc
     1621 ctggcacgtc ggcatcggcg acaacgccac cggcgacgcc acgctgctgg aggtggctcg
     1681 cgtcttctac cagcaccgcg agcagctcgc gcgcacgctg cgggtcgcct ggtggtcggg
     1741 ccactcgcac gggcgctacg ccggcagcac ctggtacgcc caggagttcg cccaggatct
     1801 ggtagccaac tgcgtggcgc acgtcaactg cgattcgccc ggctgccgct gggcgaccga
     1861 gtaccgtgac gtctacgcca tggccgaggc ggccgggctg gtcgaagccg cggtccgaga
     1921 cgtgaccggc caggcggcca gcaccgagcc gccgctgcgg gccggcgata tctccttcaa
     1981 caacctgggc atcagcacct gcttcatgct ctcctcgacc atgccgctgg agctggccag
     2041 ggagaagggc tactacgggg tcggcggctg cggcggcaac atcgcctggc acaccgagga
     2101 cgacaccatg gagatcgccg accgcgacaa cctgctgcgc gatctccgcg tctacggagc
     2161 tacgctgctg cgcctgctca acgcgccgct ctacccgctg gactaccgcg ccaccgtgga
     2221 ccagatcgcc gccatcgtcg agcggctgca gcaggccgcc ggggagaagt tcgacttcac
     2281 cgcctcgctg cgcgccgccc aagacctgcg tgccacgctc gaccgcttct atggcgaggc
     2341 cgtcgagctg agcgaccgcg aggccgacga cccggcggtg cgccaggcga acgccagcca
     2401 gcgcctgctg gcgcgctcgc tggtgacgac ggtctacagc caggacggcc gcttccgcca
     2461 ggatccggc
//
LOCUS       DASUBI010000263         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_875140, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000263 DASUBI010000000
VERSION     DASUBI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875140"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFU72_05005"
     CDS             complement(<1..631)
                     /locus_tag="VFU72_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5422878.1"
                     /translation="MEVPTVIETTNRGERVYDIYSRLLRDRIIFLGTPINDQIANAIM
                     AQMIFLEYENPEQDIRLYINSPGGSVYAGMSIYDTMQMVRCDVATYCVGLAASMAAIL
                     LAGGAKDKRFALPNSRILIHQGSAGFSGAVPDVELAARETLTMTTQVTELLARHTGQP
                     FERVKHDTERDYFMSAREAQDYGLIDHILEPNNLGLPFGQAPNGKERHGG"
     gene            complement(657..1160)
                     /locus_tag="VFU72_05010"
     CDS             complement(657..1160)
                     /locus_tag="VFU72_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5422879.1"
                     /translation="MSDIDEGFPLWLEATLRSRGLSQAQLARELGVAETQVSRWRRGL
                     AVPTVQSLQRLAEALAVPRTALEQLAGYPNGTPVFAETGHDAALAAELHAHQALLGQL
                     LAQRVPRELWQVYVAGCAALADALRSSYQETLRRAEDELAGAGGESVTRTGEQLGFRV
                     PASRGHE"
     gene            complement(1293..2270)
                     /locus_tag="VFU72_05015"
     CDS             complement(1293..2270)
                     /locus_tag="VFU72_05015"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU5422880.1"
                     /translation="MRAGRRLAYLGLVLVVALALAACGNGSSSSGTATQGRAASTTTP
                     APTTAPTAAPSPAATAAATAATAAPTTAATPAATAATTATASPTAQEQAVTAYLLQGG
                     KVWPVSRPIAHTLEVARAAMTELLAGPSQSEQAAGLSTSIPAGTTLRGVTIANGVATV
                     DLSAEFTGAGGGPLAARLAQVIYTLTQFNNVQAVTVRVGGQPLSPPPGTPVPTQSAKR
                     DDFEDETPAILIERPLLGATVTSPVRVAGSANVFEAQFVVQVQDAHGKVLKEVQARAT
                     SGTGQRGTFDLEVPFSVSGSQPGMIVAFDLSAKDGSKIDEVKVPVTLAP"
     gene            complement(2607..3545)
                     /gene="cysK"
                     /locus_tag="VFU72_05020"
     CDS             complement(2607..3545)
                     /gene="cysK"
                     /locus_tag="VFU72_05020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU5422881.1"
                     /translation="MRIANNATELVGNTPLVRLNKVVGGNATVLAKIEAFNPGFSIKD
                     RIALNMIEEAERSGELEPGTPIIEATSGNTGIGLAWVAAVKGYPVIITVSARNSPERI
                     TMLRALGAKVVVTPADQGTPGAIEEANRIAASLPKAFLPRQHWNLANPAAHYQHTAME
                     IWQDTEGRVDTFVAGFGTGGTITGVGQRLKELKPEVRIVAVEPEESPVLSQGRRGSHG
                     IQGISPGFVPEVLRPEVIDEVVLVSTDDAVAMAQRLMKEEGLLVGISSGANAAAAGRL
                     AERPENAGKVIVTVFPDLAERYLSTRLFAEAAQQGT"
     gene            complement(3630..4502)
                     /locus_tag="VFU72_05025"
     CDS             complement(3630..4502)
                     /locus_tag="VFU72_05025"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5422882.1"
                     /translation="MTALPTRDQLLVSTGWLAEHLGDPRLRILDCRYYFDRDSFQVYQ
                     EGHIPGAVYFNWNQALSDPNNPVDFMIAPPEQVEAALRRAGVDDDTLIVCYDDEGGHY
                     SSRVWLVLARYGRSDQLRILDGGWTKWLAEGRPVSSEAPQVAPGNFTLNPSQFQPELI
                     ASLEEVRRASDDGQSVILDVRRLSEFTGEEVRAKHGGRIPGAQHLLWNENLDWSGDRS
                     FHSDDELRERHEQAGLSRETPIITYCQGAVRAAHAALALGLAGYQNVRVYDGSWAEWG
                     NRDDLPIETGPARE"
     gene            4614..>4981
                     /locus_tag="VFU72_05030"
     CDS             4614..>4981
                     /locus_tag="VFU72_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5422883.1"
                     /translation="MPDTLVIRVTRQAVVLLLLALLGIWLAARLLDVLIVLLLAILLA
                     IAVAPLARRLEAGGVPRGAAILLIYVAVLGVFALTIALLVPLVNTEIDQLQAAFPGYI
                     ALLGRYGIPALNPFESTQFKS"
BASE COUNT          669 a         1764 c         1737 g          811 t
ORIGIN      
        1 ggcccccgtg ccgctctttc ccgttcggcg cctggccgaa cggcagcccc agattgttcg
       61 gctccaggat gtgatcgatc agcccgtagt cctgcgcctc ccgcgcggac atgaagtagt
      121 cgcgctcggt gtcgtgcttg acgcgctcga agggctggcc ggtgtgccgc gccagcagct
      181 cggtgacctg cgtcgtcatg gtcagcgtct cgcgcgccgc cagctcgaca tcggggacgg
      241 cgccgctgaa gccggccgag ccctggtgga tgaggatgcg cgagttcggc agggcgaagc
      301 gcttgtcctt ggcgccaccg gccagcagga tggcggccat gctggcggcc agcccgacgc
      361 agtaggtcgc cacgtcgcag cgcaccatct gcatggtgtc gtagatcgac atgccggcgt
      421 agaccgagcc gccggggctg ttgatgtaga gccggatgtc ctgctcgggg ttctcgtact
      481 cgagaaagat catctgggcc atgatcgcgt tggcgatctg atcgttgatc ggcgtgccca
      541 ggaagatgat acggtcgcgc aacaggcgcg agtaaatgtc gtagacgcgc tcaccgcggt
      601 tggtcgtctc gatgacggtg ggaacttcca tctcgctctc ctgactggtg actggctcat
      661 tcgtggccgc gtgaggccgg cacgcgaaag ccgagctgct cgccagttct tgtgaccgac
      721 tcgccgccag cgcccgccag ctcgtcctcg gcccgccgca gcgtctcctg gtaggagctc
      781 ctcagggcgt cggccagcgc cgcgcagccg gcaacgtaga cctgccacag ctcgcgcggc
      841 acgcgctgcg ccaagagctg gcccagcagc gcctggtgag cgtgcaattc cgcggccagc
      901 gccgcgtcgt ggccggtctc cgcgaagacc ggcgtgccgt tggggtagcc ggccagctgc
      961 tcgagcgcgg tgcggggcac ggccagcgcc tcggccaacc gctggagcga ctggacggtc
     1021 ggcacggcca acccacgccg ccagcgcgag acctgcgtct cggccacgcc cagctcgcgg
     1081 gcgagctgcg cctgcgacag cccccggctg cggagcgtcg cctccaacca gagcgggaac
     1141 ccctcgtcga tgtcgctcac agccgacctc cttgccgcta ccttaccagc aagcaagggt
     1201 tcttgcaaga gggatagtcg atgctggggc gtgctcggcg cggacgcgtg acggcgggta
     1261 cggcggagcc ggccagcagc ctccgccgta gctcagggcg ccagcgtgac cggcaccttc
     1321 acctcgtcga tcttcgagcc gtccttagcc gagagatcga aggcgacgat catgccgggc
     1381 tgcgacccgc tgacgctgaa gggcacttcg agatcgaagg tgccccgctg gccggtgccg
     1441 gaggtggcgc gcgcctgcac ctccttgagc accttgccgt gcgcgtcctg cacctgcacc
     1501 acgaattgcg cctcgaagac gttggcgctg ccggcgacgc gcaccgggct ggtgacggtg
     1561 gcgccgagca gcgggcgttc gatcaggatg gccggcgtct cgtcctcgaa atcgtcacgc
     1621 ttggccgact gagtgggcac cggcgtgccg ggcggcgggc tgagcggctg gccgccgacc
     1681 cgcaccgtca ccgcctgcac gttgttgaac tgcgtcaggg tgtagatcac ctgggccagc
     1741 cgcgccgcga gtgggccgcc gcccgcgccg gtgaactcgg ccgagagatc gaccgtggcc
     1801 accccgttgg cgatggtcac gccgcgcagc gtggtcccgg ccgggatgct cgtcgagagg
     1861 cccgccgcct gctcactctg gctcgggccg gcgaggagct cggtcatggc cgcgcgggcg
     1921 acctccagcg tgtgcgcgat cggccggctc accggccaga ccttgccacc ctgcagcagg
     1981 taggcggtga ccgcctgctc ctgagcggtc ggcgaggcgg tggccgtggt ggccgcggtg
     2041 gcagccggcg tcgccgccgt ggtcggggcg gcggtcgcgg cggtcgcggc ggcggtggcc
     2101 gcgggcgacg gcgccgcggt cggcgccgtg gtgggggccg gcgtcgtcgt gctggccgcg
     2161 cggccctgcg tggccgtgcc gctgctgctc gaaccgttgc cgcaggccgc cagggccagc
     2221 gccaccacga gcacgagccc caggtaggcg agccgccggc ccgctctcac tcgctcgcct
     2281 cgacccacgt tgctcatccg cgccgtcctc tccgtgcgtg accgtacaga cgattaacgc
     2341 acgggcggca aggtggggac gcacgaggag cgttcggctc tatcggcgga cgacaaggat
     2401 cggcgtaggg caagacggat ccctcgactg cgctcaggat gacagggtta gtgtccggct
     2461 gacgacgagg gtctatgccg tccgatagac ctcatctcta cacctgtcat cctgagccgg
     2521 gcggcccctc gtcctcacct gacctgaata gttacgcgca gtcgaaggat ccgtcctcgc
     2581 ccggcgccga acgtggccgt cagccgctac gtgccctgct gcgcggcctc ggcgaagagg
     2641 cgggtcgaga ggtagcgctc ggccaggtcc gggaagacgg tcacgatgac cttgccggcg
     2701 ttctccggcc gctcggccag gcgcccggcg gcggccgcgt tggccccgct ggagatgccg
     2761 accagcagcc cctcctcctt catcaggcgc tgcgccatgg cgacggcatc gtcggtggag
     2821 accagcacca cctcgtcgat cacctccggc cgcagcacct cgggcacgaa gccgggcgag
     2881 atcccctgga tgccgtgcga gccgcgccgc ccctgcgaaa ggaccggcga ctcctccggc
     2941 tcgaccgcca cgatgcgcac ctcaggcttc agctccttca atcgctgccc gacgccggtg
     3001 atcgtgccgc cggtgccgaa gccggccacg aaggtatcga cccgcccctc ggtgtcctgc
     3061 caaatctcca tggcagtgtg ctggtagtgc gccgccgggt tggccaggtt ccagtgctgg
     3121 cgcggcaaga aggctttggg caaggacgcc gcgatgcgat tggcctcctc gatcgcgccc
     3181 ggcgtgccct ggtcggcggg cgtgaccacc accttcgccc ccagcgcccg cagcatggtg
     3241 atgcgctcgg ggctgttgcg cgccgagacg gtgatgatca ccgggtaacc cttgaccgcc
     3301 gccacccagg ccagcccgat gccggtgttg ccgctggtgg cctcgatgat cggcgtaccc
     3361 ggctccagct cgcccgaacg ctcggcctcc tcgatcatgt tcagcgcgat ccggtccttg
     3421 atcgagaagc cggggttgaa ggcctcgatc ttggccagga cggtggcgtt gccgccgacg
     3481 accttgttca ggcgcaccag cggcgtattg ccgaccagct ccgtggcgtt gttggcgatc
     3541 cgcatcgact tcttccctcc gtgatggctg gctgctctcg cagaggacaa cacggccgcg
     3601 cgccgtcctc ttccccaccc ccgaggatgc tactcgcgcg ccgggccggt ctcgatcggc
     3661 aggtcgtcgc ggttgcccca ctcggcccaa ctgccgtcgt agacgcgcac gttctggtag
     3721 ccggccaggc cgagcgccag cgccgcgtgg gcggcgcgca ccgcgccctg gcagtaggtg
     3781 atgatcggcg tctcccggct gagcccggcc tgctcgtgtc gctcccgcag ctcgtcgtcg
     3841 ctgtggaagg agcggtcgcc gctccagtcc aggttctcgt tccagagcag gtgctgggcg
     3901 ccggggatgc gcccgccgtg cttggcgcgc acctcctcgc cggtgaactc gctcagccgg
     3961 cgcacgtcga ggatcacgct ctggccgtcg tcgctcgccc ggcgcacctc ctctagcgag
     4021 gcgatcagct ccggctggaa ctggctcggg ttgagcgtga agttgccggg cgcgacctgc
     4081 ggcgcctcgc tgctgaccgg gcgaccctcg gccagccact tggtccagcc gccgtcgagg
     4141 atgcgcagtt gatcgctgcg cccgtagcgc gccagcacca gccagacgcg cgaggagtag
     4201 tggccgccct cgtcgtcgta gcacacgatc agggtgtcgt cgtcgacccc agcccggcgc
     4261 agggcggcct ccacctgctc gggcggggcg atcatgaagt cgaccgggtt attcgggtcg
     4321 ctcagcgcct ggttccagtt gaaatagacc gcgccgggga tgtgaccctc ctggtagacc
     4381 tgaaagctat cgcggtcgaa gtagtagcgg cagtcgagta tgcgcagcct ggggtcgccg
     4441 aggtgctcgg cgagccagcc ggtgctgacg agcaactggt cgcgcgtggg caatgcggtc
     4501 atcttcggtc ctctctctgc tcgcggccgc gcggtggacg gccgtcccgg cagtatactc
     4561 agcccatcga ccgtgtcgat cgaagtcgat caaagggagt ggcgcgaccg tccatgcccg
     4621 acacgctggt catccgggtg acgcgacagg ccgtggtcct gctgctgctg gccctactgg
     4681 gcatctggct ggcggcgcgg ctgctcgacg tcctgatcgt gctcttgctg gccattctgc
     4741 tggcgatcgc ggtggccccg ctggcccggc ggctggaggc cggcggcgtg ccgcgcgggg
     4801 ccgccatcct gctgatctac gtcgcggtgc tgggcgtctt cgcgctgacc atcgcgctgc
     4861 tggtgccgct ggtcaatacc gagatcgacc agctacaggc cgcctttccc ggctacatcg
     4921 ctctgctcgg ccgctacggc atccccgcac tcaacccctt cgagagcacc cagttcaagt
     4981 c
//
LOCUS       DASUBI010000264         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_493843, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000264 DASUBI010000000
VERSION     DASUBI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493843"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..2014)
                     /locus_tag="VFU72_05035"
     CDS             complement(383..2014)
                     /locus_tag="VFU72_05035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5422884.1"
                     /translation="MAQVAEQAGAPVSAEQIDAIFAEWDRSDSPGCALAVVQNGEVVY
                     ARGYGMANLDLGVAITPASVFHVASVSKQFTAFAVALLAEEGKLALDDDVRKYVPELP
                     DFGQKITLRNLLQHTSGLRDQYGLFRLAGWRTGDIQAFDDVIDFARWHRRLNFAPGDE
                     YSYCNTSYTLLVLVVERVSGQRFRDFVQERLLLPLKMTHSHIHDDVEEIVPGRASAYA
                     PRKEDEGQGKGGFKVADSHVEAIGAICLYTTAEDLARWLRNLGERRVAGAVVELAATP
                     GVLNNGKQTRYGLGQMVRTYRGLKTVSHGGVDAGYRAEMLWFPEASFGVAILANLATI
                     RPGALARRVADLYLADRLGPDELRDAPAVDLPEAEQARYSGLYRDERRRLTHTVKVEA
                     GKLTINSGFGDRMELTPLGDSRFRLGEPPVGVEIVPGESGRLEVRMLEPDGDAAIFTA
                     AEAAAPTPEALAAYAGSYVCPELGGVRYSFLLRDGALVLRRRKEKDQTLEPTVADAFA
                     LPNGDLVFSRDGRGDVDGFDIYTDRIRYLRFAREA"
     gene            complement(2105..3028)
                     /gene="nikC"
                     /locus_tag="VFU72_05040"
     CDS             complement(2105..3028)
                     /gene="nikC"
                     /locus_tag="VFU72_05040"
                     /inference="COORDINATES: protein motif:HMM:NF045474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEU5422885.1"
                     /translation="MIDVDDVNKDTSAAHPARPGGLHAYKPRRRSVDFARTIYRSYPG
                     RIGGSVMLVIILLAVVAPLVAPYSADGQQIVDRLQSPSLRHFFGTDELGRDIFSRILY
                     GSRISLTVGIVAVSIALAIGVTVGLIAGYYGGVVDMLLMRFVDIMLAFPGFLLALAII
                     AMLGPSLRNAMIAVGIGSSTGFARLVRGSVLSIKENDYVMAAHVIGAPSSRIMRTHVL
                     PNALTPIIVLATLELPATILIAAGLSFLGLGAQPPSPEWGAMLVDGRSYIQTAWWLIT
                     FPGIAIFVTVLGFNLFGNALQDALNPRLRRR"
     gene            complement(3045..3968)
                     /locus_tag="VFU72_05045"
     CDS             complement(3045..3968)
                     /locus_tag="VFU72_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422886.1"
                     /translation="MAAYVLRRLILLVPIWFGILILVFLMRVLVPGDPIQLMFAGQFS
                     DPQVEAALRHKYGLDKPLTAQFVDYVDGVVHGNLGESITAQAPVTELIKSHYPYTVVL
                     TLTSLSIALVIGMVTGVISAIKKDTIIDVISMVFALAGLSMPAFWLGLLLIYIFAVDL
                     SWFPVLGSMTPRGLVLPSVTLGVIASAVTARLVRATMLDALNEDYVRTARAKGISNLR
                     VVILHALRNALIPIVTIVGLQFGGLLSGAFIVEVVFAWHGVGELAVNALKQRDFPLIQ
                     GIVLVVATTYVLVNLVVDLLYAVLDPRIAYK"
BASE COUNT          694 a         1393 c         1421 g          603 t
ORIGIN      
        1 tgagccacac atgggcagca tcggcgggca aaagggcgcc attctccgct caggtagggg
       61 cggccccccc gtggccgccg atacgcatca agttaaggat ttcgcgatcc agacgcgggt
      121 tttggcgtcg tggtgcagcg atcggcggat cgaccgaacc cgaatcgacc ggccgttcgg
      181 tctctctagc ctccaacgcc acgcggtagc cgctttggac gcggcgttcg ttcgcacctt
      241 ctccttaaac ttgatgcgta tcggcggtcc cccgtggccg ccctgttcgc cctacggccg
      301 tgcccgtcgc cgcgccgctg ggccgcccct acctgagcgg agaatggcgc ccttttgccc
      361 gccgatgcta cccatgtgtg gctcacgcct cgcgcgcgaa gcgcaggtag cggatgcggt
      421 cggtgtagat gtcgaagccg tccacgtcgc cgcgcccgtc gcgcgagaag acgaggtcgc
      481 cgttgggcag cgcgaaggca tcagccacgg tcggctccag cgtctgatcc ttctccttgc
      541 gccggcgcag caccagggcg ccgtcgcgca gcaggaagct gtagcgcacc ccgcccagct
      601 ccgggcagac gtagctgccg gcgtaggccg ccagcgcctc cggcgtcggg gcagcggcct
      661 cggccgcggt gaagatggcg gcatcgccgt ccggctccag catgcgcacc tccagccggc
      721 ccgactcgcc ggggacgatc tcgacgccga ccggcggctc acccagccgg aagcgactat
      781 cgcccagcgg cgtcagctcc atgcggtcgc cgaagccgct gttgatcgtc agcttgccgg
      841 cctcgacctt gacggtgtgc gtcaggcggc ggcgctcgtc gcggtagagc ccgctatagc
      901 gcgcctgctc ggcctccggc agatcgaccg ccggggcgtc gcgcaactcg tcggggccga
      961 gccggtcggc caggtagaga tcggccaccc gtcgcgccag cgcccccggc cggatcgtgg
     1021 ccagattggc caggatggcg acgccgaagg acgcctcggg gaaccagagc atctcggcgc
     1081 ggtagccggc gtcgacaccg ccatggctga ccgtcttcag cccgcgatag gtgcggacca
     1141 tctggcccag gccatagcgc gtctgcttgc cgttgttgag cacgccgggg gtggcggcca
     1201 gctcgaccac cgcgccggcc acccggcgct cgcccaggtt gcggagccac ctggccagat
     1261 cctcggcggt ggtgtagagg cagatggcgc cgatggcctc gacgtgggag tcggccacct
     1321 tgaagccacc cttgccttga ccctcgtcct ccttacgggg agcgtaggcg ctggcgcggc
     1381 cgggcacgat ctcctcgacg tcgtcgtgga tgtggctgtg cgtcatcttc agcggcagca
     1441 gcaagcgctc ctggacgaag tcgcggaagc gctggccgct gacgcgctcg accaccagca
     1501 ccagcagcgt atagctggtg ttgcagtagg agtactcgtc gcccggagcg aagttgagcc
     1561 ggcggtgcca gcgggcgaag tcgatcacat cgtcgaacgc ctggatgtcg ccggtgcgcc
     1621 agccggccag gcggaagagg ccgtactggt cgcgcaggcc gctggtgtgc tgcagcaggt
     1681 tgcgcagcgt gatcttctgg ccgaagtccg gcagctccgg cacatatttg cgcacgtcgt
     1741 cgtcgagcgc gagcttgccc tcctcggcca gcagcgccac cgcgaaggcg gtgaactgct
     1801 tggagaccga ggcgacgtgg aagaccgagg cgggggtgat cgccaccccg agatcgagat
     1861 tggccatgcc gtagccgcgc gcgtagacga cctcgccgtt ctggacgacc gccagcgcgc
     1921 agccgggcga gtcggagcga tcccactcgg cgaagatggc gtcgatctgc tcggcgctga
     1981 ccggcgcgcc cgcctgctct gcaacctgtg ccatgtctcc ccacttcctg tggtcgtacg
     2041 cggcggaccg accgtcgcga atcaccgggc gtcgcgcggc gcccgctgct ctcgaagcgc
     2101 gaacctagcg ccgccgcagg cgcgggttga gcgcgtcctg cagcgcgttg ccgaagagat
     2161 tgaaaccgag cacggtgacg aagatggcga tgccggggaa ggtgatcagc caccaggcgg
     2221 tctggatgta ggagcggcca tcgaccagca tggcgcccca ctcgggcgag ggcggctgcg
     2281 cccccaggcc gaggaaggag agcccagccg cgatcagaat cgtcgccggc agctccagcg
     2341 tggccagcac gatgatcggc gtcagcgcgt tgggcagcac gtgcgtgcgc atgatgcgcg
     2401 agctgggcgc gccgatcacg tgcgctgcca tgacgtagtc gttctccttg atcgagagga
     2461 ccgagccgcg caccagacgc gcgaagccgg tggaggagcc gatgccgacc gcgatcatgg
     2521 cgttgcgcag gctggggccg agcatggcga tgatcgccag cgccagcagg aagccgggga
     2581 aggccagcat gatatcgacg aagcgcatca gcagcatgtc gaccacgccg ccgtagtagc
     2641 cggcgatcag gccgacggtg acgccgatcg ccagcgcgat cgagaccgcc acgatgccga
     2701 ccgtcaggct gatgcgcgag ccgtagagga tgcggctgaa gatgtcgcgc cccagctcat
     2761 cggtgccgaa gaaatgccgc agcgagggcg actgcaagcg gtccacgatc tgctgtccgt
     2821 ccgccgagta gggcgcgacc agcggggcga cgaccgccag caggatgatc accagcatca
     2881 cgctgccgcc gatgcggccg ggatagctac ggtagatcgt gcgcgcgaag tcgacgctgc
     2941 ggcgccgggg cttgtaggcg tgcagcccgc cgggccgcgc cgggtgcgct gcgctcgtgt
     3001 ccttgttcac atcatccacg tcgatcatcg ctcctccagc ccccctactt atacgcgatg
     3061 cgcggatcga gcaccgcgta gagcagatcg acgaccagat tgaccagcac gtaggtcgtc
     3121 gccaccacca gcacgatccc ctggatcaag gggaaatcgc gctgcttgag cgcgttcacc
     3181 gccagctccc cgacgccgtg ccaggcgaag acgacctcga cgatgaaggc gccggagagg
     3241 agcccgccga actgcaggcc gacgatagtc acgatcggga tcagcgcgtt gcgcagggcg
     3301 tgcaggatga cgacccgcag gttcgagatc cccttggcgc gcgcggtgcg cacgtagtcc
     3361 tcgttgagcg cgtcgagcat ggtggcgcgc accaggcggg cggtgaccgc cgaggcgatc
     3421 acgccgagcg tcaccgaagg gagcaccagg ccccggggcg tcatcgagcc gagcaccggg
     3481 aaccagctca gatcgacggc gaagatgtag atcagcagca ggcccagcca gaaggccggc
     3541 atcgacagcc cggccagcgc gaagaccatc gagatgacgt cgatgatcgt gtctttcttg
     3601 atcgccgaga tgaccccggt caccatgccg atgaccagcg cgatcgagag gctggtgagt
     3661 gtcagcacga cggtgtaggg atagtgtgac ttgatcagct cggtcacggg cgcctgagcc
     3721 gtgatcgact cgcccaggtt gccgtggacc acgccgtcga cgtagtccac gaactgggcc
     3781 gtcagcggtt tgtcgagccc gtacttgtgg cgcagggcgg cctcgacctg cgggtcactg
     3841 aactgcccgg cgaacatcag ttggatcggg tcgccgggca ccaacacccg catcaggaag
     3901 accaggatca ggatgccgaa ccagatcggg acgagcagga tcaggcgacg aagcacatac
     3961 gcggccaccg gtcttccatt ccttcactag tcgcgctcgg gggcggccct cacggaggtc
     4021 cctcaccccc agcccctctc ccgtgcgcgg gcgaggggcg ttgcccagcg agcaggctta
     4081 gcatcaggga aatatcccta acaccaaacc t
//
LOCUS       DASUBI010000265         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_434686, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000265 DASUBI010000000
VERSION     DASUBI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434686"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="VFU72_05050"
     CDS             complement(<1..1598)
                     /locus_tag="VFU72_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5422887.1"
                     /translation="MATTADLLVERLIDWGVDTIFGLPGDGINDIFESLRTHQDKIRF
                     IQVRHEESAAFAACGYAKYTGRPGVCLATSGPGGIHLLNGLYDAKLDGQPVLAITGHT
                     FHDLIGTHYQQDVDLDKLFMDVSAYNERVMGPHHVVSVVDEAMKTLLGRRSVAHITIP
                     KDIQGWSKDDEPRSSADIPGHSGEIFAARHSLPPQEMLQRAAELINAGEKVAILAGRG
                     ALDARAEVLALADAAAAPIVKPLLGKAVVPDDNPFTTGGIGLLGTGASYDVMQDADTL
                     VILGSTFPYMEFYPKPGQARTVQVDIDPNRIGLRHPVEVGLIGDCRSVLQALLPMIQR
                     KSKRDFLKDAQSKMEDWRKLMQERGTRGGTPMKPQVVAYQLNRFLPDNTIICCDTGTV
                     TTWAARHLDIRGDMQFSASGMLATMACGLPYAVGAGAAYPDRPVLAFVGDGGFSMLMA
                     EMATLVKYNLPVKIVLIKNNVLGQIKWEQIVFEGFPQYGVELQPIDFAAYARACGANA
                     ATLDDPDKAEETVRQFMETPGPGLL"
     gene            1914..>2571
                     /locus_tag="VFU72_05055"
     CDS             1914..>2571
                     /locus_tag="VFU72_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEU5422888.1"
                     /translation="MRFTRRVACLVMLLALVAGSFPFFASADSPPLPPGTITTTTFDD
                     DASPNNGTCSLREAVAAVNSQQKVDQCPAGKAIALAAGTYHLTVKAGTAGASSTGALG
                     ISGNVTITSAGAGLTTIDATGLGDGVFNVSAKATLTLANLTIRGGNSSDDGGAIVNTG
                     TLSLSGVVLTANHGGGNGGAILNSGGTLNLSTTLLSQNSASGDGGGLAIPGGTVTIAA
                     S"
BASE COUNT          419 a          857 c          885 g          410 t
ORIGIN      
        1 agcagcccgg ggccaggcgt ctccatgaac tgccggacgg tctcctcggc cttgtccgga
       61 tcgtcgagcg tggcggcgtt ggcgccgcag gcgcgggcgt aggccgcgaa gtcgatcggc
      121 tgcagctcga cgccgtactg cgggaagccc tcgaagacga tctgctccca cttgatctga
      181 cccaggacgt tgttcttgat cagcacgatc ttgaccggca ggttgtactt caccagcgtc
      241 gccatctcgg ccatcagcat cgagaagccg ccatcgccga cgaaggccag caccgggcga
      301 tcgggatagg cggccccggc gccgacggcg taggggaggc cgcaggccat cgtcgccaac
      361 atgcccgagg cggagaactg catgtcgcca cggatatcga gatggcgcgc ggcccaggtg
      421 gtcaccgtgc cggtgtcgca gcagatgatg gtgttgtccg gcaggaagcg gttgagctga
      481 taagcgacca cctggggctt catcggcgtg cctccgcgcg tgccgcgctc ctgcatcagc
      541 ttgcgccagt cctccatctt cgactgcgcg tccttcagga agtcgcgctt ggacttgcgc
      601 tggatcatgg gcagcagcgc ctgcagcacc gagcgacagt cgccgatcag accgacctcg
      661 accgggtgcc gcaggccgat gcggttgggg tcgatgtcta cctggaccgt ccgggcctgc
      721 ccgggcttgg gatagaactc catgtagggg aaggtgctgc cgaggatcac cagcgtatcg
      781 gcgtcttgca tcacgtcgta ggaggcgccg gtgccgagca ggccgatgcc gccggtggtg
      841 aagggattgt cgtcgggcac gaccgccttg cccagcagcg gcttgacgat cggcgcggcg
      901 gcggcgtcgg ccagggccag cacctcggcg cgggcgtcga gcgcgccgcg cccggccagg
      961 atcgcgacct tttcgccggc gttgatgagc tccgccgcgc gttggagcat ctcctggggc
     1021 ggcaggctgt ggcgcgcggc gaagatctcg ccgctgtggc cggggatatc ggccgaggag
     1081 cgcggctcgt catccttcga ccagccctgg atatccttgg ggatggtgat gtgcgccacg
     1141 ctgcggcggc cgagcaacgt cttcatcgcc tcgtcgacca cgctgacgac gtggtgcggg
     1201 cccatcacgc gctcgttgta ggccgacacg tccatgaaga gcttgtcgag atcgacatcc
     1261 tgctggtaat gcgtgccgat caggtcgtgg aaggtgtggc cggtgatcgc cagcaccggc
     1321 tggccgtcga gcttggcgtc gtagaggcca ttcagcaggt gaatgccacc ggggccggac
     1381 gtcgccaggc agacaccggg acgaccggtg tacttggcat agccgcaggc cgcgaaggcg
     1441 gccgattctt cgtggcgcac ctggatgaag cgaatcttgt cctgatgggt gcgcagcgac
     1501 tcgaagatat cgttgatgcc gtcgccgggg agcccgaaga tcgtgtccac gccccagtcg
     1561 atcagccgct cgacgagaag gtccgccgtg gtcgccatga gccgctctcc ttgatctcgc
     1621 gttgtgccac gccagtccgc gcgccccgag agcagcaatt tgtgtaccga ggatcgcggc
     1681 acgaggtggt gccggcggtt cagtgtcgca gcgcgacaca ctatccccag gtgaacaggt
     1741 actttcggac tttgctcgca gcgtgacatg atgttataag acagaggtac cgaatcgcgt
     1801 tagccatttc gtcaccggta ggtggcgtct cgcgacgtgg cggagcgggc gcgccaggga
     1861 cgacggagcc ccggccgatg ggggtgtgcg gcgcgcaatg ggaggggaag ttgatgcgtt
     1921 tcacgcgacg cgtggcctgc ctggtcatgc tcttggcgct cgtggccggt tcgttcccat
     1981 tcttcgccag tgccgactcc ccaccacttc ctcccggcac catcaccacc acgaccttcg
     2041 acgacgacgc cagccccaac aacggcacct gctcgctgcg cgaggcggtg gccgcggtca
     2101 acagccagca gaaggtcgac cagtgccccg ccggtaaggc gatcgcactg gccgccggca
     2161 cctaccacct gaccgtcaag gccggcacgg cgggcgccag ctcgaccggc gcgcttggaa
     2221 tctccggcaa cgtgaccatc accagcgcgg gcgccggcct gacgaccatc gacgccaccg
     2281 gcctgggcga cggcgtcttc aacgtcagcg ccaaggcgac gctgacgctc gcgaacctga
     2341 ccattcgcgg cggcaacagc agcgacgacg gcggcgccat cgtcaacacc ggcaccctga
     2401 gcctgagcgg cgtcgtgctg accgccaacc acgggggcgg caacggcggc gccatcctca
     2461 acagcggcgg cacgctcaac ttgagcacca cgctgctcag ccagaacagc gccagcggcg
     2521 acggcggcgg gctcgccatc cccggcggca cggtgaccat cgcggccagc g
//
LOCUS       DASUBI010000266         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_107456, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000266 DASUBI010000000
VERSION     DASUBI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107456"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..492
                     /gene="rplL"
                     /locus_tag="VFU72_05060"
     CDS             118..492
                     /gene="rplL"
                     /locus_tag="VFU72_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5422889.1"
                     /translation="MDQIIQAIENMTVLQLSQLVKALEERFGVSAAAPVAVAAAPGAA
                     GAAPAEEAEEKTAFDVVLTEIGPNKIQVIKAVRELTSLGLKEAKDLVESAPKPVKEGV
                     NKEEAEAAKAKLEEAGAKAEVK"
     gene            584..660
                     /locus_tag="VFU72_05065"
     tRNA            584..660
                     /locus_tag="VFU72_05065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:618..620,aa:Pro,seq:ggg)
     gene            712..1311
                     /locus_tag="VFU72_05070"
     CDS             712..1311
                     /locus_tag="VFU72_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5422890.1"
                     /translation="MEKPHAPARVVIADDESIIRMDLREMLTHLGYEVVGEAADGRTA
                     LELVDKLRPDLVVLDIKMPQLDGIEAARELAVDHIAPVILLTAYSEQHLVDRAKDAGV
                     AGYLVKPFRESELLPVIELALSQFEQMRRLESEVDELKGALETRKLVERAKGILMEVH
                     GLKEAEAFSRMRRASMDHRKSMREIAEAILLSHHVENNP"
     gene            complement(1533..1952)
                     /locus_tag="VFU72_05075"
     CDS             complement(1533..1952)
                     /locus_tag="VFU72_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEU5422891.1"
                     /translation="MRPRHRPAWARLRHFFDRGNIARAVVSLILAFLLWAWVTAENNP
                     QVNRLVGGIQVSSVNLSDQLQVESQLPTVDIRLEGPRDELNALDPLRIQVTVDLGDVK
                     QPSTVTVPVHVTVPGHLRVREVAPSKVSVTVARSGTK"
     gene            complement(1921..2814)
                     /gene="cdaA"
                     /locus_tag="VFU72_05080"
     CDS             complement(1921..2814)
                     /gene="cdaA"
                     /locus_tag="VFU72_05080"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU5422892.1"
                     /translation="MPHLPWIFTRLDLRALIDILAVASIFFWVLWVAQGTRATQLIRG
                     LVILVVLVVGAANIFDLTALKWLLDKTLPAFIIAIPIVFQPELRRALEQLGHTGSWLR
                     PPFTAQPEEQLEHMVDEIGRATSQLSRYRYGALIVIERETGLQEYVVRGVPLDANLTW
                     QLLINIFYPNSPLHDGAVIIRGDTILAASCVLPLSENVTGSQLGTRHRAGLGISEESD
                     AVAVIVSEETGRISVAHNGRLVRDLDQERLRRILRTLLRLDRYERPRPPPRPPAAEGG
                     ERPPDMERGIEDAAQTSARVG"
     gene            complement(2904..>4432)
                     /locus_tag="VFU72_05085"
     CDS             complement(2904..>4432)
                     /locus_tag="VFU72_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5422893.1"
                     /translation="LDLERLRIHTYPTPEGLLHLATAALDAGRTDLANSYVPAAIEIA
                     FPAAGSAQALKLAESWVTQQPDNWVAKVLLALELVAAGRLDAVLPLLTPPPVDADTRS
                     LVAALAVAAVVDSPEQWSLAARLWERVPGNRQELKAIRANFELAANKTGFQVVLWIHM
                     GLIELAAGNCKTAREHLLSHRPPDALRAYAALLGAVAAALQGHDDAAASAALKHAAPL
                     HARPEVADFAARVHCVPGGRGLIELGIELAERLIAHDDINAALGVLESLSDAAPRNAA
                     LTKRYAELLESAGQQEQALSRLTQLLARQEQARDTEGAQHTLRGMLQLDPTNQQLRER
                     LIESYLRRGNIEGAVEERCKVAERLAERGRTEEALNQLLQALEVASLTTDWRSLERIF
                     TLVVRIAPNDVDLRHRAITAAIEHGKLGEAVRHLWDVVRICSDNLDLDETIAALHQII
                     ALAPSDSDAYHRLGEALAAIGEFQQAERVYRRLQTLLPDDAAVRAKQIALAALARENQ
                     "
BASE COUNT          749 a         1433 c         1530 g          720 t
ORIGIN      
        1 gcgccgaagc cgcctagcaa gtcgctcgcc ggagacgcga tccggcattg gaaccgtcgg
       61 ctagcgcgcc ccgcgcgcgg agagggagac acaatcatgg cagtggatca gcagaacatg
      121 gaccagatca tccaggcgat cgagaacatg acggtgctgc agttgtcgca gttggtgaag
      181 gcgctggagg agcgcttcgg cgtcagcgcc gcggcgccgg tggccgtcgc cgccgcgccg
      241 ggcgccgccg gtgcggcccc cgccgaggag gccgaggaga agacggcctt cgacgtggtg
      301 ctgaccgaga ttggcccgaa caagattcag gtcatcaagg ccgtgcgcga gctgaccagc
      361 ctgggtctga aggaggccaa ggacctggtc gagtcggcgc ccaagccggt caaggagggc
      421 gtcaacaagg aggaggccga ggcggccaag gccaagctgg aagaggccgg cgctaaggcc
      481 gaggtcaagt agccgcgatc gccggcaggt tggccgcgtc gcaaccggcg cggcccagcc
      541 cctgcgggcc agcccgatgg tatgctatac tccagccaat attcggggcg tagcgcagcc
      601 cggtagcgca ccggtatggg gtaccggcgg tcggaggttc gaatcctctc gccccgacca
      661 cgttctagct gattccccct cgcatctgca cgcaacctga gggattgcga tatggaaaag
      721 ccccacgccc ccgctcgcgt ggtcatcgcc gatgacgagt cgattatccg catggatttg
      781 cgcgagatgc tgacgcacct cggttacgag gtggtcggcg aggcggcgga cggccgcacc
      841 gcgctcgagc tggtggacaa gctgcggccg gacctggtag tcctcgacat caagatgccg
      901 cagctggacg gcatcgaggc cgcgcgagaa ctggcggtag atcacatcgc gccggtcatt
      961 ctgctgacgg cctacagcga gcagcatctg gtggaccggg ccaaggatgc cggtgtcgcc
     1021 ggctacctgg tcaagccctt ccgcgagagc gagctgctgc ccgtgatcga gctggcgctg
     1081 tcgcaattcg agcagatgcg gcgtctggag agcgaggttg acgagctgaa gggcgcgttg
     1141 gagacgcgca agctcgtcga gcgcgccaag ggtatcctca tggaggtgca cggcctgaaa
     1201 gaggccgagg cgttcagtcg catgcgccgc gccagcatgg accaccgcaa gtcgatgcgc
     1261 gagatcgccg aggcgatcct cctgtcccat cacgtcgaga acaacccctg agcccggcgt
     1321 tcctctccca tttctgcctg tcgacatgaa tgattatcac tcgaggacgg atccctcgac
     1381 tgcgctcggg atgaccgtgt gagtgagggg ctcatacgga ggaacgtcct cgttcgagag
     1441 gcttcatcct gttccctgtc atccccggcg cagtcgaggg atctgttctg ggccgtgtcc
     1501 agccgttgtc gaaagccgcg cctcggtgac gctcacttcg tgccactgcg ggcgacagtg
     1561 accgagacct tggacggcgc gacctcccgc acgcgcaggt ggccggggac ggtgacgtgt
     1621 accgggacgg tcaccgtgct gggctgtttc acgtcgccga ggtcgaccgt gacctgaatt
     1681 cggagcggat cgagcgcatt cagctcgtcg cgcggcccct cgagtcggat atcgacggtt
     1741 gggagctgcg actccacctg gagctggtcg gagaggttga cggacgacac ttgaatcccg
     1801 ccgaccaggc gattgacctg gggattgttc tcggccgtga cccaggccca cagcaggaaa
     1861 gccagaatca acgacaccac cgcgcgcgcg atgttgccgc gatcgaaaaa gtgacgcagc
     1921 ctagcccacg cgggccgatg tctgggccgc atcttcgatc cctcgttcca tatccggcgg
     1981 ccgctcaccg ccttcggcag cgggcggcct aggtggtggg cgtgggcgtt cgtagcgatc
     2041 gagccgcagg agggtgcgca ggatgcggcg caggcgctcc tgatcgagat cgcgtaccag
     2101 gcgtccgtta tgcgccaccg agatgcgccc ggtttcttcg gagacgatca ccgccaccgc
     2161 atcggattcc tcagagattc ccaatccagc acggtggcgg gtgccgagtt ggctgccggt
     2221 tacgttctcg ctcaagggga gcacacagct ggcggcgaga atcgtatcgc cccggatgat
     2281 gaccgcccca tcgtggagcg gcgagttcgg gtagaagatg ttgatcagga gttgccaggt
     2341 cagattggca tcgagcggca ccccacgaac gacatactcc tgcaggccgg tctcacgctc
     2401 gatcacgatc aacgcgccgt agcggtaacg cgagagctgg gacgtggccc ggccgatctc
     2461 gtcgaccata tgctccaact gctcctccgg ttgggcggtg aagggcggcc gcagccagga
     2521 gccggtgtgg ccgagttgct ccagtgcacg gcgtagctcg ggctggaaca cgatcgggat
     2581 ggctatgatg aaggccggca gcgtcttgtc cagcagccac ttgagcgcgg tgaggtcgaa
     2641 gatgttggcc gcgccgacga ccagcaccac caggatgacc agaccgcgga tgagctgcgt
     2701 agcgcgcgtg ccctgagcca cccagagcac ccaaaaaaag atcgacgcga cggccagaat
     2761 atcgatgagc gcgcgaagat cgagcctggt gaagatccac gggagatggg gcatgctgtc
     2821 atcctgtgcc ggtcgcgtac gcaccagtgg tcacgattgt atcatgaccc tcccccgttc
     2881 ccgcctcagt tcagcgccgc gtgctactgg ttctcccgcg cgagcgccgc cagcgcgatc
     2941 tgcttggcgc gtaccgcggc gtcatccggc aagagcgtct gcagccgccg gtaaacgcgc
     3001 tcggcttgct ggaactcacc gatcgccgcc agcgcttcgc ccagacggtg ataggcgtcg
     3061 gagtcgctgg gcgccagcgc gatgatctgg tggagcgccg cgatcgtctc gtccagatcg
     3121 aggttgtcgc tgcagattcg caccacgtcc cagagatggc ggacagcctc gccgagcttg
     3181 ccgtgctcga tggcggcggt gatggcgcgg tgccgcaggt ccacatcatt cggcgcgatc
     3241 cgcaccacca gcgtgaagat tcgctccagg ctgcgccaat cggtcgtgag gctggcgacc
     3301 tccaacgcct gcaggagctg gttgagcgcc tcctcggttc gcccgcgctc cgccaaccgc
     3361 tcggcgacct tgcagcgctc ctcgacggcc ccctcgatat tgccccgccg caggtagctc
     3421 tcgatcagtc gttcgcggag ctgctggttg gtgggatcga gctgcagcat gccgcgaagc
     3481 gtgtgctgcg cgccctcggt atcacgcgcc tgctcctgtc gcgccagcag ctgcgtcaag
     3541 cgtgatagcg cctgctcctg ctgcccggcg ctctccaaca gttcggcgta gcgcttggtc
     3601 agcgcggcat tgcgcggcgc ggcatcgctg aggctctcca gcacgcccag tgcggcgttg
     3661 atgtcgtcgt gcgcgatcag gcgttccgcg agttcgatgc ccagctcgat cagcccgcgg
     3721 cctcccggca cgcagtgcac ccgggcagcg aaatccgcca cctcgggccg ggcatgcagg
     3781 ggtgccgcgt gcttcagcgc ggcgctggcg gcggcatcgt cgtgaccctg gagagccgcg
     3841 gcgaccgcgc cgagcagagc ggcgtacgcc cgcaacgcat ccggtggccg gtggctgagc
     3901 aggtgctcgc gcgccgtctt gcagttgccg gcagccagct cgatcaagcc catatggatc
     3961 cagagcacga cctggaatcc ggtcttgttg gccgccagct cgaagttggc ccggatggcc
     4021 ttcagctctt ggcgattgcc gggcacgcgc tcccagagcc gggccgccag cgaccactgt
     4081 tccgggctat cgaccaccgc cgccacggcc aacgcggcga cgagcgagcg ggtgtcggca
     4141 tcgaccggcg gaggggtgag cagaggaagt accgcatcca ggcgcccggc ggcgacgagc
     4201 tccagcgcca agagcacctt ggctacccag ttgtccggct gttgggtgac ccagctctcg
     4261 gcgagcttca gcgcctgcgc tgagccggcg gccggaaagg cgatctcgat cgcggcaggc
     4321 acatagctgt tggccaggtc ggttcggccg gcgtcgagcg cggcagtggc caggtggagt
     4381 agtccctccg gcgtggggta ggtgtggatg cgcaggcgct cgagatcgag cg
//
LOCUS       DASUBI010000267         7072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_423452, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000267 DASUBI010000000
VERSION     DASUBI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423452"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFU72_05090"
     CDS             complement(<1..342)
                     /locus_tag="VFU72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5422894.1"
                     /translation="MSSDHRTPYLFVSYASADRERVLALVARLEAVGVDIWIDRDGIH
                     GGANYAQVISDAIKGAAALLLAASPAALASRNVRQELALGWRYEKPYLPLLLDPVEIP
                     DELAYWLEGSQW"
     gene            complement(662..1867)
                     /locus_tag="VFU72_05095"
     CDS             complement(662..1867)
                     /locus_tag="VFU72_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5422895.1"
                     /translation="MTEVQAGERARRTATRDRRRLFVAMMTTQGAFGGFQTLWPLYIA
                     ALGASPSQVGLVIGLVEAMRLGALLPVGVLGERFAPKWVITGGLLLKVLALVLYALAG
                     TWWQLLPAGFLFAVGAIFPAILAEIAAVAGDGPERVRTFTLMNTVAPAIGLLIAPAIA
                     GLIAARASLHGVFLFAALLTAAAAGLFATLAPRPPAPAQPVAVTYRSTLAQQAIRRWL
                     LLEMAAVFALGLGITLIPNYLHDYQGVSNGAIGFFASLSAAGSIVLGLLVHRVPALRQ
                     PVRGVTLALGASALALLLVLAGQSLPAFVAAYVLIGGFFATWTLFEAALSGVTPAAYR
                     ARTYALAELMSGAGISLAPFIAGVLYERDPRAPVLAGLVCIVTLVAAMFAMDRRGALP
                     ASHLPEEAT"
     gene            complement(1932..3776)
                     /locus_tag="VFU72_05100"
     CDS             complement(1932..3776)
                     /locus_tag="VFU72_05100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5422896.1"
                     /translation="MPTSNEHWSREPGRWETALSPVAYAMVPSISTPCPAPDGRRVAY
                     SRGYDGRNDLWVVDASGGLPVQLTDRAALQGPDPNQRHATAIAWTPDGRHLIFASNKD
                     GKLWQVPAAGGPSRVIDEGPGNHHSPAVAPDGTRVAYVAERGEQGDIVVADIEGHSLR
                     LVSAGDEYVLQPRWSPDSRRLLYGQWPHYDMPWDERALVVADVASGERRIIAGGERVT
                     NADAVWSPDGASIAFVSDREGEFGNLWTCDADGANARRLVAEEAQHATPAWAPDGRRI
                     AYTRNDDGDCQIWLWQDGQTRQLTHEPGVHGELSWLGGERILCSFSSPVRPTDLYLID
                     AASGERRQLTQSATGGILGAEPVMPEHVAWQSSDGLTVHGLLFTPREQRPGQHPLVIS
                     IHGGPVGQTLPNWQGHVQYLVDRGYVVLAPNYRGSKGYGRGFMEKLYGDWGGGDLQDN
                     ISGAKMVIERGLVDPRKVIAMGGSAGGYSTLICMTKAPDFFRAGVCRFGIGDLATFTD
                     KTWIFERHYIAKLMGAPGANSALYYDRSPVHHVANVREPLLILQGEEDIVCHPSQMNE
                     MVEALRAAGKDVEYHTYPGEGHGWRKVATIVDDAERIDDFLVRKVLNR"
     gene            complement(3830..4867)
                     /locus_tag="VFU72_05105"
     CDS             complement(3830..4867)
                     /locus_tag="VFU72_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5422897.1"
                     /translation="MTETTEQPVRFGLSLPNRAVLFGLPVETLLASAEQADRSGFFDS
                     VWVGDNFLSKPRLEAIVTLSALAARTRHLRLGTICLASFPLRHPLPLALQWASLDVLS
                     GGRTILAVCLGGSARMGPKFAQELAALGVRSNERAGRLEEGIELLRLLWGPEPVSYHG
                     RFYTLDDVVLQPKPVQPRIPIILAVNPPEQADPAVEERALRRVARLADGWQTDGTPPA
                     VFARRWARIREYAHEFGRADEVRDASLHLMVNIDDDAGRARREAVEFLAHYYGSGAIS
                     DEKLASWLAFGPPVAVAEKIAAFVAAGCTTVVVRFTALDQQGQLDRFLAEVAPLLRQQ
                     FAIVCGAPQAR"
     gene            complement(4883..6094)
                     /locus_tag="VFU72_05110"
     CDS             complement(4883..6094)
                     /locus_tag="VFU72_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEU5422898.1"
                     /translation="MPAPDPAAIAGLVETHLERGYLVETLARLAAISTAVEPGFDTLM
                     EPDDPKLVAYVQGVLRPELVRQGAYDLLDVPRNNLVVRLGSGASGRSLLIQNYTPSQH
                     QQETDPHLAGRVGNARAYGCDEPAVFGQGVSQNKAHQAVMLAVIGLLLRSGVQLRGRL
                     YWAVNNEGRSSHACSDAIVAALGERPAFGVIQIGTGLNISLGNRGRVDVDVHLRGRAV
                     HSSTPEQGLSAIEGAHEVISRLRALRWADRHPLLGGRHAIVYKLRYAPVLPHTLPAEA
                     FLTIDRRLLPGDDPAAAAEEVRAAIGDLAPYQVQVTQGVFMLPALVDAEHPGVRALQA
                     ANRAVRGHEAEEYYGAGTFDAGGPNAHGIPTVMFGASGRSTVTGTDFVTIAAVEAEAR
                     VLAHLILSELG"
     gene            6476..>7072
                     /locus_tag="VFU72_05115"
     CDS             6476..>7072
                     /locus_tag="VFU72_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422899.1"
                     /translation="MKRDITFGDLLRELLEERLGPRSAKALAVRLGVDPSLVRRWLRN
                     ARVPALDSPYLDLIVATLGLTPSEREHLRQAQIDMLSVPPKERRRFSPASQVRQLLTT
                     GHQPQPQPELRAATGPLQPAYYGPGALLRAFQDAVAALPPAHGPQPPILFALNGSWTP
                     RRTSEEIMQLWMRALSRLLELGWDWTVVGGPRMPGTNRQ"
BASE COUNT          999 a         2494 c         2574 g         1005 t
ORIGIN      
        1 ccactgcgag ccttccagcc aataggccag ctcgtccggg atctcgaccg ggtcgagcag
       61 gagcggcagg tacggcttct cgtagcgcca gccgagggcc agctcctgcc gcacgttgcg
      121 cgaggcgagc gcggcgggcg aggcggcgag gaggagggcc gccgccccct tgatggcatc
      181 actgatcacc tgagcgtagt tggcgccgcc atggatgccg tcgcggtcga tccagatgtc
      241 gacccctacc gcttcaagcc gcgcgaccaa cgccagcact cgctcccggt cggcgctggc
      301 gtagctgacg aacaggtacg gagtccggtg atcgctagac ataacatccc tcggccgctc
      361 gtgtagcatc ggttgaggct cccatttgac cgcgccggag atgtcgcgtc aagatacctt
      421 tgggaggttc ggctggtcac gccacgcgcg gccgcgaagc cggagcagct tggcctggct
      481 cggggagcct ggttgagcca aaagccgcgc tcccgctctt gcaccgggat ccgttggacc
      541 ggctcctcgt cgcccaagcg ctgctggagg agttgccgct cctgagcgtc gaccccgctg
      601 tggcccaata caacgtcctt gtcatctggt gaaccggcgc cgccgctcca tccgaaacgg
      661 gctacgtagc ttcctctggc agatggctcg ccggcagggc gccgcggcga tccatcgcga
      721 acatggccgc gaccagggtg acgatgcaga cgaggccggc cagcaccggg gcgcgggggt
      781 cgcgctcgta gaggaccccg gcgatgaagg gcgccagcga gatgcccgcg ccgctcatca
      841 gttcggccag cgcgtaggtg cgcgcgcggt aggccgccgg ggtgacgccg ctgagcgccg
      901 cctcgaagag ggtccaggtg gcgaagaagc cgccgatcag gacgtaggcc gccacgaagg
      961 cgggcagcga ctgcccggcc agcaccagca gcagcgccag cgcggaggcg ccgagcgcca
     1021 gcgtcacccc gcgcaccggc tggcgcagcg ccggcacgcg gtggaccagt aggcccagca
     1081 cgatgctccc cgccgccgag agcgaggcga agaagccgat ggcgccgttg gagacgccct
     1141 gataatcgtg caggtagttg ggtatcagcg tgatacccag ccccagcgcg aagaccgcgg
     1201 ccatctccag cagcagccag cgccggatgg cctgctgggc cagcgtcgag cggtaggtga
     1261 cggccaccgg ctgggccggc gccgggggcc ggggcgcgag ggtggcgaag aggccggcgg
     1321 cggcggccgt cagcagcgcg gcgaagagga agacaccgtg cagcgaggcg cgcgccgcga
     1381 tcaggccggc gatcgccggg gcgatcagca gcccgatcgc gggggccacg gtgttcatca
     1441 gggtgaaggt gcgcacccgc tccgggccat cgccggccac cgcggcgatc tcggccagaa
     1501 tggcggggaa gatcgcgccc accgcgaaga ggaagccggc cggcaggagc tgccaccagg
     1561 tgcccgccag ggcgtagagc accagcgcca gcaccttcag cagcaacccg cccgtgatga
     1621 cccacttggg cgcgaagcgc tcgcccagca cgccgacggg gaggagcgcg ccgaggcgca
     1681 tcgcctccac cagcccgatg accagcccca cctgcgaggg cgaggcgccc agcgcggcga
     1741 tgtagagcgg ccagagcgtc tggaagccgc cgaaggcgcc ctgggtagtc atcatggcca
     1801 cgaagaggcg ccgccggtcg cgcgtggcgg tccgccgcgc ccgctccccc gcctgtactt
     1861 cagtcaagct ctacgccagc ttcgcgatgt ctagccgcct cccgcgttcc gccgcgccgc
     1921 ttcgccagcc ttcagcggtt cagcaccttg cgcaccagaa aatcgtcgat gcgctcggcg
     1981 tcgtcgacga tcgtcgcgac cttgcgccag ccgtgcccct cgccggggta ggtgtggtat
     2041 tcgacgtcct tgccggcggc acgcagcgcc tcgaccatct cgttcatctg gctgggatgg
     2101 cagacgatgt cctcctcgcc ctgcaagatc agcagcggct cgcgcacgtt ggccacgtgg
     2161 tggaccgggc tgcggtcgta gtacagcgcg ctgttggcgc caggcgcgcc catcagcttg
     2221 gcgatgtagt ggcgctcgaa aatccaggtc ttgtcggtga aggtcgccag atcgccgatg
     2281 ccgaagcggc agacgccggc gcggaagaag tcgggcgcct tggtcatgca gatcagcgtg
     2341 ctgtagccac cggcgctacc gcccatcgcg atcaccttgc gcgggtcgac cagcccccgc
     2401 tcgatgacca tcttggcccc gctgatgttg tcttgcaggt cgccgccgcc ccagtcgccg
     2461 tagagcttct ccatgaagcc gcggccatag cccttgctgc cacggtagtt gggcgccagc
     2521 accacgtagc cgcgatcgac caggtactgg acgtgcccct gccagttggg gagcgtctgc
     2581 cccaccggac cgccgtggat cgagatgacc agcgggtgct ggccggggcg ctgctcgcgc
     2641 ggggtgaaga gcagcccgtg cacggtcagc ccatcgctgc tctgccaggc gacgtgctcg
     2701 ggcatcaccg gctcggcgcc caggatgccg ccggtggcgc tctgggtgag ctggcgacgc
     2761 tcgccggatg cggcgtcgat caggtagaga tcggtgggcc ggaccggcga tgagaagctg
     2821 cacaggatgc gctcgccgcc gagccagctc agctcgccgt gcacccccgg ctcgtgggtg
     2881 agctggcgcg tctggccgtc ttgccagagc cagatctggc agtcaccgtc gtcgttgcgc
     2941 gtgtaggcga tgcggcggcc gtcgggcgcc caggcgggcg tggcgtgctg cgcctcctcc
     3001 gccaccagcc ggcgcgcgtt ggccccgtcg gcgtcgcagg tccagaggtt gccgaactcg
     3061 ccctcgcggt cggagacgaa ggcgatggac gcgccgtcgg gcgaccagac ggcgtcggcg
     3121 ttggtgacgc gctcgccccc ggcgatgatg cgccgctcgc cgctcgcgac gtcggccacc
     3181 accagggcgc gctcgtccca gggcatgtcg tagtgcggcc actggccgta gagcagccgg
     3241 cggctatcgg gcgaccagcg tggctgcagc acgtactcgt cgccggcgct cacgaggcgg
     3301 agactatgcc cctcgatgtc ggccacgacg atgtcgccct gctcgccgcg ctcggccacg
     3361 taggccacgc gcgtgccgtc gggcgcgacg gcaggcgagt ggtggttgcc gggcccctcg
     3421 tcgatgacgc gcgacggccc gcccgcggcc ggcacctgcc acagcttgcc gtccttgttg
     3481 gaggcgaaga tgagatgccg gccgtcgggc gtccaggcga tcgcggtggc gtggcgctgg
     3541 ttggggtccg gcccctgtag cgcggcccgg tcggtcagtt gtacgggcag accaccgctg
     3601 gcatcgacga cccagagatc gttgcgcccg tcgtagccgc ggctgtaggc cacccgccgg
     3661 ccatcgggcg cggggcaggg ggtggagatc gagggcacca tggcgtaggc cacagggctg
     3721 agcgccgtct cccagcgccc cggctcgcgg ctccagtgct cattcgaggt cggcatgtcg
     3781 actccctttc ccatctattc gcgcgtggcc cgcccgccgc gggcgccgct caacgtgctt
     3841 ggggcgcccc gcacacgatc gcgaactgct gccgcagcag cggcgcgacc tcggccagga
     3901 agcggtcgag ctggccctgc tgatcgagcg cggtgaagcg cacgacgacc gtggtgcagc
     3961 cggccgccac gaaggcggcg atcttctcgg ccacggccac gggcgggccg aaggccagcc
     4021 aggaggccag cttctcgtcg gagatggcgc cgctgccgta gtagtgcgcc aggaattcga
     4081 ccgcctcgcg ccgggcgcgg ccggcgtcgt cgtcgatatt gaccatcagg tgcaggctgg
     4141 cgtcgcggac ttcgtcggcg cgcccgaact catgggcgta ttcccggatg cgggcccagc
     4201 gccgggcgaa gacggcgggc ggcgtgccgt cggtctgcca gccgtcggcc aggcgcgcga
     4261 cccggcgcag ggcacgctcc tcgacggccg ggtcggcctg ctccggcggg ttgaccgcca
     4321 ggatgatcgg gatgcgcggc tggactggct tgggctggag cacgacatcg tccagtgtat
     4381 agaagcggcc gtggtagctg accggctccg gtccccagag aagacgcagc agttcgatcc
     4441 cctcctccag cctcccggcg cgctcgttgg agcgcacgcc cagggcggcc agctcctggg
     4501 cgaacttcgg ccccatgcgc gccgacccgc ccaggcagac cgccaggatg gtgcgcccgc
     4561 cggagaggac atccaggctg gcccactgca gcgccagcgg cagcggatgg cgcagcggaa
     4621 agctggccag gcagatggtg ccgagccgca ggtggcgggt gcgcgcggcc agggcgctga
     4681 gcgtgacgat cgcctccagc cgcggtttcg agaggaagtt gtcgcccacc cagaccgagt
     4741 cgaagaagcc ggagcggtcg gcctgctcgg cgctcgccag cagcgtctcg accggcagtc
     4801 cgaagaggac ggcgcggttg ggcaggctga gcccgaagcg gaccggctgc tcggtggtct
     4861 ccgtcacgtc acttcgctcc tctcagccca gctcgctcag gatcaggtgt gccagcacgc
     4921 gcgcctcggc ctcgaccgcg gcgatggtga cgaagtcggt gccggtgacg gtcgaccggc
     4981 cgctggcgcc gaacatgacc gtcgggatgc cgtgggcgtt gggcccgcca gcgtcgaagg
     5041 tgccggcgcc gtagtactcc tccgcctcgt gcccgcgcac ggcccggttg gcggcctgca
     5101 gcgcgcgcac gccggggtgc tcggcgtcca ccagcgccgg cagcatgaag acgccctggg
     5161 tcacctggac ctgatagggc gccagatcgc cgatggcggc gcgcacttcc tcggcggcgg
     5221 cggcggggtc gtcgcccggc agcaggcggc ggtcgatggt caggaaggcc tcggccggca
     5281 gggtgtgcgg caggaccggc gcgtagcgga gcttgtagac gatggcgtgg cgcccgccga
     5341 gcaggggatg gcggtcggcc cagcgcagcg cgcgcagccg gctgatgacc tcgtgcgcgc
     5401 cctcgatggc gctcagcccc tgctccggcg tgctggagtg gaccgcccgg ccacgcaggt
     5461 gcacgtcgac atcgacccgg ccgcgattgc ccagcgagat gttcaatccg gtgccgatct
     5521 ggatgacgcc gaacgcgggc cgctccccca gcgccgccac gatcgcgtcg gagcaggcgt
     5581 ggctgctgcg cccctcgttg ttgaccgccc agtagagccg accgcgcagt tggaccccgc
     5641 tccgcagcaa gagcccgatg accgccagca tgaccgcctg atgcgccttg ttctggctga
     5701 cgccctgccc gaagacggcg ggctcgtcgc agccgtaggc gcgcgcgttg ccgacgcggc
     5761 cggccaggtg cgggtcggtc tcctgctgat gctggctggg ggtgtagttc tggatcagga
     5821 gcgagcggcc gctcgcgccg ctgccgagcc gcacgacgag gttgttgcgc ggcacgtcga
     5881 gcaggtcgta ggcgccctgg cgcaccagct cggggcgcag cacgccctgc acgtaggcga
     5941 ccagcttggg gtcgtccggc tccatcagcg tgtcgaagcc cggctcgacc gcggtcgaga
     6001 tcgccgccag ccgcgccagg gtctcgacca ggtagccccg ctcgaggtgc gtctcgacga
     6061 gtcccgcgat ggcggccggg tctggcgcgg gcatgcgctc ctctccctct gatcgacgtc
     6121 cgcggctctc cgccgcggcc aggccgcgtc cccgtctgtg cctgcccatg gtagcgcccg
     6181 gcggacggct gtcaacgacc gcgcgggctc cggccacgca cgtcgacgaa caggacttca
     6241 gcgccacgtg tttggcaccg atggccgttt cttcggtgcc gtgagcccgc taggctgagg
     6301 gagcggggtt gcgtcatgcc cacagctcgc gcgcgtcgac gctggacggc gaggcgagac
     6361 gcgggcgcat cggtgttgcg atactgcgcc cggcttgcgg gccaatattg tcctgtttgc
     6421 aaggctcggt ttgtctccgg gtcgcgttag gatgggggcg gggaagcgga gggtgatgaa
     6481 gcgcgacatc acattcggtg acctgctgcg tgagctgctc gaggagcggc tcggtccacg
     6541 cagcgcgaag gcgctggctg tccgcctcgg cgtcgatcca tcgctggtgc gccgctggct
     6601 gcgcaatgcc cgcgtgccgg cgctggactc gccctatctc gatctgatcg tcgccacgct
     6661 cggcctgaca ccatcggagc gtgagcacct gcgacaggcg cagatcgaca tgctctcggt
     6721 cccccccaaa gagcgccggc gcttctcccc agcgagtcag gtgcgccagc tcctcaccac
     6781 gggacaccag ccccagccac agcccgagct gcgcgccgcg accggtccgc tgcagcctgc
     6841 ctactacggg ccgggcgccc tgctgcgcgc ctttcaggat gcggtggcgg cgctgccccc
     6901 ggcgcacggc ccccagccgc cgatcctgtt cgcgttgaat ggttcctgga caccgcgccg
     6961 gaccagcgaa gagatcatgc agctctggat gcgcgccctc tcgcgcctgc tggagctggg
     7021 gtgggattgg accgtggtcg gcggcccccg catgccgggc accaaccggc ag
//
LOCUS       DASUBI010000268         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1066605, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000268 DASUBI010000000
VERSION     DASUBI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066605"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..997)
                     /locus_tag="VFU72_05120"
     CDS             complement(176..997)
                     /locus_tag="VFU72_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422900.1"
                     /translation="MSITAFEGSGRARPGAWRRLAWTIEDALTMVKRNLLHIPRNPEL
                     LLDVTVQPIIFVLLFRYVFGGAINVPGGSYVNYLMAGIFVQTIVFSALATGIFLANDM
                     QKGLIDRFRALPMARSAVLAGRTLTDLLRGLLAVVIMLVVGILVGFRPSGGADGWLAG
                     LGLLLLFGFALAWVGVTAGVLLRSPESVQAAMFVAVFPLTFASSAFVPTQTMPHWLRA
                     FAANQPMTLVVNAIRDFTLGQPAGATAWEALAWIIGIIVIFAPLSVALYQRKGLS"
     gene            complement(994..2010)
                     /locus_tag="VFU72_05125"
     CDS             complement(994..2010)
                     /locus_tag="VFU72_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5422901.1"
                     /translation="MSNNGLAVVAEDLHKHFGETQALRGVDLAIAEGSILAVLGPNGA
                     GKTTAVRILSTLLKADSGHAEVAGFDVAREPEEVRRRIGLTGQYAALDEFLTGRENLE
                     MIGRLYHLPRALVRRRALELLERFDLTDAGSRLVKTYSGGMRRRLDLAASLIAAPPVL
                     FLDEPTTGLDPRSRLGMWGLIEELVAGGTTLLLTTQYLEEADRLAHQIAVIDHGLVIA
                     RGTADELKTQVGGERLILTLAPGSDLPAAARVLDHLAVGPLQLAEPQYQLVIPVAASS
                     GLLTQVVRELDAIGVAVDDLQLHRPTLDDVFLTLTGHAAEDGVGEGEPGVDTVEQRME
                     VGAA"
     gene            2210..>2535
                     /locus_tag="VFU72_05130"
     CDS             2210..>2535
                     /locus_tag="VFU72_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422902.1"
                     /translation="MIVATSGRERVLQSARELFFTRGYAEVSMQQIAESVGLTKAALY
                     YHFRDKDDLFAQVVVREMTHQRQGFAREIARGGTLGEILERLAHSYIAQLSPDVVRIM
                     VDFKQHV"
BASE COUNT          416 a          867 c          891 g          361 t
ORIGIN      
        1 cacggttacg cgcagcgcca cacccgctgc cagcgtcgca ccggccaccg gcgccgtgcc
       61 ggccataacc acgtcgtagg aggggcagac gcggtccctg tcctctggcc cgccggcgcc
      121 gtgcaacctg ggccgcgatg ctcgcttgtc cgccggcgcg gcccaccgcg cctggctagc
      181 tcaacccctt gcgctggtag agcgccaccg agagcggggc gaagatcacg atgatgccga
      241 taatccaggc gagtgcttcc caggcggtcg cgccggctgg ctggccgaga gtgaagtcgc
      301 ggatggcgtt gaccaccagc gtcatcggct ggttggccgc gaaggcgcgc agccagtgcg
      361 gcatcgtttg ggtgggcacg aaggcgctgc tagcgaaggt gagtgggaag accgccacga
      421 acatggccgc ctgcaccgac tccgggctgc gcagcagcac gcccgccgtg actccgaccc
      481 aggccagggc gaagccgaag aggaggagca ggccgaggcc ggccagccag ccgtcggcgc
      541 cgccggacgg gcggaagccg accaggatgc cgaccaccag catgatgacg accgccagca
      601 ggccgcgcag cagatcggtc agcgtgcgcc cggccagcac cgccgagcgc gccatcggca
      661 gcgcgcggaa gcggtcgatc agtcccttct gcatgtcgtt ggcgaggaag atcccggtcg
      721 ccagcgcgga gaacacgatc gtctggacga agatgccggc catcagatag ttgacgtagc
      781 tcccaccggg cacgttgatc gcgcccccga agacgtaacg gaagaggagc acgaagatga
      841 tcggctgcac cgtgacatcc agcagcagtt ccgggttgcg cggaatgtgc agcaggttgc
      901 gcttgaccat ggtcagcgcg tcctcgatgg tccaggcgag ccgtcgccag gcgcccggac
      961 gcgcccggcc cgatccctca aacgcggtga tgctcatgcc gcgccaacct ccatccgctg
     1021 ctcaaccgtg tctacccctg gctcgccctc accaacgccg tcttcggcgg catggccggt
     1081 cagggtcagg aagacgtcgt ccagggtcgg gcggtggagc tgcagatcgt cgaccgcgac
     1141 gccgatcgcg tccagctcgc gcacgacctg ggtcaatagc ccggaactgg ccgccaccgg
     1201 aatcaccagt tgatactgcg gctcggcgag ctgaagcggg ccgaccgcca ggtgatcgag
     1261 cacgcgcgcg gcggccggca ggtcgctgcc cggcgccagg gtcaggatca gccgctcacc
     1321 gcccacctgc gtcttgagct cgtcggcggt gccgcgcgcg atcaccagcc cgtggtcgat
     1381 gaccgcgatc tggtgcgcca gccggtcggc ctcctccaga tactgggtgg tcagcagcag
     1441 cgtcgtgccg ccggccacca gctcctcgat cagcccccac atgcccaagc ggctacgcgg
     1501 atcgagcccg gtggtcggct cgtcgaggaa gaggacgggc ggcgcggcga tcaggctggc
     1561 ggccagatcg agccgccggc gcatgccgcc ggagtaggtc ttgaccaggc gtgagccggc
     1621 atcggtcagg tcgaagcgct ccagcagctc gagcgcgcgg cggcgcacca gggcgcgcgg
     1681 caggtggtag agccggccga tcatctccag gttctcgcgc ccagtgagaa actcgtcgag
     1741 cgccgcgtac tggccggtca ggccgatgcg tcggcgcacc tcctccggct cgcgcgcgac
     1801 gtcgaagccg gcgacctcgg cgtggccgga gtcggctttc aagagcgtcg agaggatgcg
     1861 caccgcggtg gtcttaccgg cgccattggg gccaagcacc gccaggatgc tcccttcggc
     1921 gatggcgagg tcgacaccgc gcagcgcctg cgtctcgccg aagtgcttgt gcagatcttc
     1981 ggcgacgacg gccagtccat tgttgctcat gcggcctcct ggtaccgggt ggtggggcca
     2041 gccgcttgac gcactgaccg cccggtaagt agactggtgg cgacggtcgc tctgggggga
     2101 gcggtcgcct aactaaccgg tcggtaagta ctctaatggg cggtgacgcc gttgtcaacc
     2161 ctgcccgggg ggagaacgcg gcccgcgccg ccgagataga gacggggcga tgatcgtcgc
     2221 gaccagcggg cgtgagcgcg ttctgcagtc ggcgcgcgag ctcttcttca cgcgtggcta
     2281 tgccgaggtg tcgatgcagc agatcgccga gtccgtcggc ctgaccaagg cggccctcta
     2341 ctaccatttc cgcgacaagg acgacctctt cgcgcaggtg gtggtgcgcg agatgaccca
     2401 ccagcgccag ggcttcgcgc gcgagatcgc acgcggcggg acgctgggcg aaattctgga
     2461 gcgcctggcc cacagctaca tcgcgcagct cagccccgac gtggtacgca tcatggttga
     2521 tttcaagcag cacgt
//
LOCUS       DASUBI010000269         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1120877, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000269 DASUBI010000000
VERSION     DASUBI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120877"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFU72_05135"
     CDS             <1..722
                     /locus_tag="VFU72_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU5422903.1"
                     /translation="EGKMVRRILLGAGVVSSLLYIAADIIGTLRYPGYSWLDQEFSEL
                     TAQGAPTRPLMIGLVEFPYNLLVLALALGLWTTAGPHRRAARLAAGALAGYTAFGFVA
                     GTITPMATREAMAAGQDTLRNAFHGPLTMVSDLFIAAGMGFAGQLLGKRFRYYSYATI
                     ATLIVFGAVTALQIPQMQANEPTPWMGLEERVNIYATMLWVALLAVGLWRAEAREESS
                     QLGKPSATPPQLPVGRGRTPA"
     gene            749..1489
                     /locus_tag="VFU72_05140"
     CDS             749..1489
                     /locus_tag="VFU72_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU5422904.1"
                     /translation="MIAVRQTLRRTLERHPTVVKVLLGCGVIASAWWVAADVIASLRY
                     PGYSYLDQTVSELSAEGAPTRAFLMAVNAVPFAALLAAFGLGVWALSRARAGRVIGGL
                     LAGSAVVGLLGGILFPMMMRGAEGDLRNAMHAPYGAVSMLLFVLMFGFGAKLLGTRFR
                     YYTYGTIAVWFVFGVLMALQSGRMVADEPTPWMGLEERISVYATMLWFAVLSVGLLRT
                     EAAGGSRSPGQPAAVPPTKQPDRGKLPA"
     gene            1516..2268
                     /locus_tag="VFU72_05145"
     CDS             1516..2268
                     /locus_tag="VFU72_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU5422905.1"
                     /translation="MIALPQSPRRARPRPVTITKVLLVGGVVAPVWWVIMDVVGSLRY
                     PGYSYVDQTISELSAQGAPTRTFMLVLSGIPYTVLLLAFAAGVWRAAGARRAGRVTAA
                     LTAVEALWGFVGGIAFPMATREVIAAGQDTLRNQMHAWYGLGMPLLFALVIGFGSRLL
                     GKRFRYLSYATLLTMLVFGLLMGLQTGAMTANQPTPWMGVEERVTGYAPMLWFVVLAI
                     GLVRAGAADSPHRPWRPVSAEPPRPSRARLVR"
     gene            complement(2368..>3360)
                     /locus_tag="VFU72_05150"
     CDS             complement(2368..>3360)
                     /locus_tag="VFU72_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5422906.1"
                     /translation="AWGQMSVAALNISGWWDMNFPGAHLNFSGMRARARSDVLRTGQK
                     LIIGPWPHRVNRSRTLNGLDFGEQAIINLEQYQLRFYDRWLKGVDNGIDAEPPVYVFV
                     LGANEWRADWDWPPSDAEPVPFYFHSRGHANRLTGDGVLSTEPPGEEPEDAYRYDPRD
                     PNGMLWRIADGPVDDRLPAIRDDLLCYTTEVLDEPLTVVGPVSVTLYAASSARDTDWH
                     VRLCDVHPDGSARFLCHGMLRARFRESFEQPRLLDPGEVYAFTFGADACGVRFLPGHR
                     IRVEVTSSWFPEYDRNTNSGAENNFLDDQVVVATNRIFHERGRASHVTLPVVRG"
BASE COUNT          481 a         1114 c         1193 g          572 t
ORIGIN      
        1 gagagggaaa gatggtacgc aggatcctgc tgggcgcggg cgtcgtctcg tcgctgctct
       61 atatcgccgc cgacatcatc ggcacgctgc gctaccctgg ctacagctgg ctcgatcagg
      121 agttcagcga gttgacggcg cagggcgcgc cgacgcggcc gctcatgatc gggctggtcg
      181 agttccccta taacctgctg gtgctggccc tggccctggg cctgtggacg acggccggcc
      241 cgcaccggcg cgcggcgcgc ctcgccgcgg gcgcgctggc cggctatacc gccttcggct
      301 tcgtggccgg cacgatcacc ccgatggcca cgcgtgaggc gatggccgcc ggccaggata
      361 cgctgcgcaa cgccttccac ggtccgctga cgatggtgag cgatctcttc atagcagcgg
      421 gcatgggctt tgccggccag ctgctgggga agcggttccg ctactactcg tacgcaacca
      481 ttgcgaccct tatcgtcttc ggcgcggtga ccgccctgca gatcccacag atgcaggcga
      541 acgagccgac gccctggatg gggttggagg agcgcgtcaa catctacgcc accatgctct
      601 gggtggcgct gctggcggtt ggcctctggc gggctgaagc acgcgaggaa tccagtcagc
      661 tcgggaagcc ctcggcgact ccaccgcagc tgccggtagg tcgcggccgg acgccggcct
      721 gagccgagcg ccggagggag gatcagcatt gattgccgta cgccagacgt tgcgccgtac
      781 cttggaacgt caccccacgg tcgtcaaggt gctgcttggc tgcggggtca tcgcgtcggc
      841 gtggtgggtg gcggcggatg tgatcgcgag cctgcgctat cccggctaca gctacctcga
      901 tcagaccgtc agcgagctct cggcggaggg ggcgccaacg cgcgccttcc tgatggcagt
      961 gaatgccgtt ccgttcgccg ccctgctggc ggccttcgga ctcggcgtct gggcgctgag
     1021 ccgggcccgc gcgggacgcg tcatcggcgg cctgctggct ggaagcgcgg tcgtcggcct
     1081 gttgggtgga atcctcttcc cgatgatgat gcgcggtgcc gagggagatc tgcgcaacgc
     1141 catgcacgcc ccctatgggg cggtgagcat gctcctcttc gtactcatgt ttggcttcgg
     1201 ggcaaagctg ctgggcacgc ggttccggta ctacacctac gggaccatcg ccgtctggtt
     1261 cgtatttggc gtcctgatgg ctctgcagtc gggccggatg gttgccgacg agccgacgcc
     1321 ctggatgggg ctggaggagc gcatcagcgt ctacgccacc atgctctggt tcgcggtgct
     1381 ctcggtcggc ctgctgcgaa ctgaagcggc cggcggatcc cggtctcctg ggcagcctgc
     1441 ggcggttccg ccgacaaagc agcccgaccg cggcaagctg ccggcctgac gctcgtgccg
     1501 gaaggaggag tcacaatgat tgctctgcca cagtcgccgc gccgtgccag gccgcgcccc
     1561 gtgacgatca ccaaggtgct gctcgtcggc ggggtcgtcg cgccggtatg gtgggtcatc
     1621 atggacgtcg tggggagcct acgctacccc ggctacagct acgtcgatca gaccattagt
     1681 gagctgtcgg cgcagggagc gccgacgagg acctttatgc tggtgctcag cggcatcccg
     1741 tataccgtgc tgctgctcgc ctttgccgcc ggcgtctgga gagccgcggg cgcgcgccgc
     1801 gccggccgcg tcaccgccgc gctgacggcc gtcgaggccc tctggggctt cgtgggcggg
     1861 atcgccttcc cgatggccac ccgcgaggtc atcgcggcgg gccaggacac gctgcgcaac
     1921 cagatgcacg cgtggtacgg gctggggatg ccgctcctct tcgcactggt catcggcttc
     1981 gggtccaggc tgctcggcaa gcggttccgg tatctctcgt atgcgaccct cctcaccatg
     2041 ctcgtgtttg gcctgttgat gggcctacag accggcgcca tgaccgccaa ccaaccgacg
     2101 ccgtggatgg gggttgagga gcgcgtcacc ggctacgcgc cgatgctctg gttcgtggtg
     2161 ctggccatcg gtctcgtgcg tgccggcgcg gccgactcgc ctcaccggcc ctggcggccg
     2221 gtgagcgcgg agccaccgcg gccatctagg gcacgtctcg tcagatgatt gcgggcgagc
     2281 cgcgcttggt cccgtcagcg gcgcggccgc ggcgttggcg aagagcgacg gcgttctcgg
     2341 gcgggaatcc gccggcgacc gtcgccgcta tccgcgcacg accggcaagg tgacgtgcga
     2401 tgccctcccc cgctcgtgga agatgcgatt ggtggcgacg accacctggt cgtcgaggaa
     2461 gttgttctcc gcgcccgagt tggtattgcg gtcgtactcg gggaaccacg aactggtcac
     2521 ttccacgcga atgcggtgac ccggcaggaa gcgcaccccg caggcgtcgg ccccaaaggt
     2581 gaaggcgtag acctcgcccg gatcgagcaa ccgcggctgc tcgaacgact cgcggaaacg
     2641 cgcccgcagc atgccgtggc agagaaagcg cgccgagccg tcaggatgca catcgcacag
     2701 ccgcacgtgc cagtcggtgt cgcgcgcgga cgaggcggcg tagagcgtga ccgagaccgg
     2761 gccgacgacg gtgagcggct cgtcgagcac ctcggtggtg tagcacaaca ggtcgtcgcg
     2821 gatcgcgggt aggcggtcgt ccaccgggcc atcggcgatc cgccagagca tgccgtttgg
     2881 atcacgcggg tcgtagcggt aggcgtcttc gggctcctcg ccgggcggct cggtcgagag
     2941 cacgccgtcg cccgtcagcc gattggcgtg gccccgcgag tggaaataga acggcaccgg
     3001 ctcagcatcg gatggcggcc agtcccagtc ggcccgccat tcattggccc cgagcacgaa
     3061 gacgtacacg ggcggctcgg cgtcgatgcc gttgtcgacg cccttgagcc agcgatcgta
     3121 gaagcgcagt tggtactgct ccaggttgat gatggcctgc tcgccgaagt ccagcccgtt
     3181 gagggtgcgg ctgcggttca cccggtgcgg ccaggggccg atgatgagct tctgacccgt
     3241 gcgcaggaca tcgctgcgcg cccgcgcgcg catgccgctg aagttcaggt gtgcgccggg
     3301 gaagttcatg tcccaccagc cgctgatatt gagcgccgcg accgacatct gcccccaggc
//
LOCUS       DASUBI010000270         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_309239, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000270 DASUBI010000000
VERSION     DASUBI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309239"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2064)
                     /locus_tag="VFU72_05155"
     CDS             complement(<1..2064)
                     /locus_tag="VFU72_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5422907.1"
                     /translation="MVVKAEPKLVTLNINGQDITVPDGSTVLDACLSHGIEVPNLCFQ
                     PLLRPWGSCRMCIVEILGRRGGLVESCAAQVREGMEVATHSEEVLKSREFLLQMYLID
                     HALDCPTCDKSGECYLQDNTYLHNVQVSPFNRPKLAQPYEHFSDVIDYKWDRCIICAR
                     CTRVCDEVIGVTAIEIDGRGLEASVTTAWDMDLRDTTCISCGMCIAVCPVGAMTDRKF
                     GEHPWLMDATETICGFCDVGCTLSVEHNRGIIRRVSHLWDRGVNHGYTCERGKWGYQF
                     VQNPERLEDAAIRRDGVLQAVPLDEALDEAAARLSHYQGEQFAALASADDTNESNYLL
                     QQFARAVMGSNNVDRTMTHNQAAVDRALTDSFGLGASTNSVQEMFTDTGCALVVGPNI
                     VDAAPVASYWVNWGRLYRETKDIVISRDHFVMCDRAQVWIKPPENGETAIINAIARVI
                     LDNKMLTPPASPPAGYQAWLDTLQDYAPEDVAKSTGVPLADIQRAAVLYATGGHEVSD
                     DPSGYGPSTIFSTLASQIGGDVYPAELALHNLALLTGNIGRPGGGVIAFRGHSNFQGS
                     TDAGCHPALLPGYQALDDAEALRKFSAAWLPRWNDDVVPRNGFKRPQSLPTARGLGVE
                     EIIQAIDDGRIKALYLAFGSQPKNYGFDARLLEVLPKLELLIVEDIYPSELTGLAHIV
                     LPAAMF"
BASE COUNT          357 a          724 c          788 g          398 t
ORIGIN      
        1 gaacatcgcg gccggcagca cgatgtgggc cagcccggtc agctcgctgg ggtagatatc
       61 ctccacgatc agcagctcga gcttgggcag cacctccagc agccgggcgt cgaagccgta
      121 gtttttgggc tgactgccaa aggcgaggta gagcgctttg atgcggccgt cgtcgatcgc
      181 ctggatgatc tcttcgacgc cgagaccgcg cgcggtgggc aggctctgcg ggcgcttgaa
      241 accgttgcgc ggcacgacgt cgtcgttcca gcgtggcagc caggcggcgc tgaacttccg
      301 cagcgcctcg gcgtcatcga gtgcctggta gccgggcaag agcgccgggt ggcagccggc
      361 atcggtgctg ccctggaagt tggagtggcc gcggaaggcg atcaccccac cgcccgggcg
      421 cccgatgttg ccggtcagga gcgccaggtt gtgcagcgcc aactccgccg ggtaaacatc
      481 gccgccgatc tgactcgcca gcgtgctgaa gatggtgctt gggccgtagc cgctcgggtc
      541 gtcggagacc tcatggccgc cggtcgcgta gagcaccgcc gcgcgctgaa tatcggccag
      601 cggcacaccg gtcgatttgg cgacatcctc cggcgcgtaa tcctgcagcg tgtccagcca
      661 cgcctgatag ccggccggcg gactggccgg cggtgtcagc atcttgttgt cgaggatgac
      721 gcgagcgata gcgttgatga tggccgtctc gccgttctcc ggcggcttga tccagacctg
      781 cgcccgatcg cacatcacga agtggtcgcg gctgatgacg atatccttcg tctcgcggta
      841 gaggcgcccc cagttgaccc aatagctggc gaccggcgcg gcgtcgacga tattcggacc
      901 gacgaccagc gcgcagccgg tgtcggtgaa catctcctgc accgaattgg tgctggcgcc
      961 cagaccgaac gagtccgtca gcgcgcgatc gaccgccgcc tggttgtggg tcatggtgcg
     1021 atcgacgttg ttgctgccca tgacggcgcg ggcgaactgc tgcagcaggt agttgctctc
     1081 attggtatcg tccgctgagg ccaacgccgc gaattgctca ccctggtagt ggctcaggcg
     1141 cgcggccgct tcgtctaacg cctcgtccag cggtacggcc tgcagcacgc cgtcgcgtcg
     1201 gatggcggca tcctccagcc gctccgggtt ctgcacgaac tgatagcccc atttgccacg
     1261 ctcacaggtg taaccgtggt tgacgccgcg gtcccagaga tggctgacgc ggcgtatgat
     1321 gccgcggttg tgctcgacac tcagcgtgca gccgacgtcg cagaagccgc agatcgtctc
     1381 ggtcgcgtcc atcagccagg ggtgctcgcc gaatttacgg tcggtcatgg cccccaccgg
     1441 gcagacggcg atgcacatac cgcaggagat gcaggtggtg tcgcgcaggt ccatgtccca
     1501 ggcggtagtc acgctcgcct ccagcccgcg gccgtcgatc tcgatggcgg tgacgccgat
     1561 cacctcatca cagacgcgcg tgcagcgggc gcagatgatg cagcggtccc acttgtagtc
     1621 gatcacgtcg ctgaagtgct cgtagggctg ggccagtttg gggcggttga aggggctgac
     1681 ctggacgttg tggaggtagg tgttgtcctg caggtagcac tcgcccgact tgtcgcaggt
     1741 ggggcagtcg agcgcgtggt cgatgaggta catctgcagg aggaactcgc gcgacttgag
     1801 cacctcctcc gagtgggtgg cgacctccat cccctcgcgc acctgcgcgg cgcacgactc
     1861 gaccagaccg ccgcgacgcc cgaggatctc gacgatacac atgcggcagg agccccaggg
     1921 gcgcagcagc ggttggaagc agaggttggg cacctcgatg ccgtgcgaca ggcacgcgtc
     1981 gagcaccgtg ctcccatcgg gaacggtgat gtcttgcccg ttgatattga gggtgacgag
     2041 cttgggttca gccttgacga ccatgcggcg gacctgcctc cccatcggat tcggcgagcg
     2101 gggcgtcgcg cgggaggctc gcgccggtcg gggtgcggag gaggtggcgg cgtccggcgg
     2161 gtggccccgg cgagcatgcc ctcgatcgcc gattctacca tagcgcccgc gtttgcccgc
     2221 gatttcacaa tcggaggtcg cgcgatagtc ctcaggaacc aggccgc
//
LOCUS       DASUBI010000271         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1053692, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000271 DASUBI010000000
VERSION     DASUBI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053692"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFU72_05160"
     CDS             complement(<1..228)
                     /locus_tag="VFU72_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422908.1"
                     /translation="MFSSAARRLAILNAAVVIVVVLLLGGTTFFALRHELNAEVDSSL
                     RGRLKPVVKQWAESGLNGASASQQAKPDDDIE"
     gene            complement(232..903)
                     /locus_tag="VFU72_05165"
     CDS             complement(232..903)
                     /locus_tag="VFU72_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5422909.1"
                     /translation="MRILVVEDEVGLADVIARVLRRERYEVDIAHDGEQGLELALAGI
                     YDAIVLDRMLPGIDGLELSRRLRREAVHTPVLILSARRELDERVEGLDAGADDYLGKP
                     FAFTELLARLRALTRRGERPLLTAVLRAGGVTLDQRTHRVERAGQPVELSPREYALLE
                     YLMRNAGQVLTRDQILERVWGYEAQPESNVVDLYIYYLRRKLGDEGAPSPIQTVRGVG
                     YTMRA"
     gene            complement(923..1609)
                     /locus_tag="VFU72_05170"
     CDS             complement(923..1609)
                     /locus_tag="VFU72_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422910.1"
                     /translation="MVIESPAKPAGAARHGPLAAPLGVLLVALGIAGLLAAALLLGFS
                     PSPATWYLARASGFTLYLLLWWSVVSGLGLTTSLLGLGGRAAAWDAHRFTTELAFVFL
                     ALHLLSLAIDPTVPLGLLGVLVPLQSAVRQPWTDLGILTGYGMIIITASFALRRFIGQ
                     RGWRLLHYGAFPLWLIALAHGLGAGSDAHRLWAQLLYLASVAVVIFLLAFRLLRRGRA
                     PGGGGASGPV"
     gene            complement(1830..>2190)
                     /locus_tag="VFU72_05175"
     CDS             complement(1830..>2190)
                     /locus_tag="VFU72_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422911.1"
                     /translation="TSMIIMMSTMGTTMTTTDLDALRAPRAGLAPRGRVATMERHARL
                     KKLILAGALGSFLGLFGLTVVQDRQSLPVAAGAVPPATSVLPSSAGASAGQQTSGTVR
                     LAAPVKKAKPKVRTSSS"
BASE COUNT          344 a          792 c          754 g          300 t
ORIGIN      
        1 ctcgatatca tcgtcgggct tggcctgctg gctggcgctc gcgccgttca gcccgctctc
       61 ggcccactgc ttcaccaccg gcttcagccg cccgcgcagc gagctatcga cctcggcgtt
      121 gagctcgtgg cgcagcgcga agaaggtcgt gccgcccaag agcagcacca cgacgatgac
      181 gacggccgcg ttgaggatgg ccagccggcg cgcggcgctg ctgaacacgg ctcacgctcg
      241 catggtgtag ccgacgccgc gcacggtctg gatcggagag ggcgcgccct cgtcgccgag
      301 cttgcgccgc agataataga tgtagagatc gaccacgttg ctctcgggct gcgcctcgta
      361 gccccagacg cgctccagga tctggtcgcg cgtcagcacc tggccggcgt tgcgcatcag
      421 gtattcgagc aaggcgtact cgcgcgggct cagctcgacc ggctgaccgg cgcgctcgac
      481 ccggtgggtg cgctggtcga gcgtgacgcc gccggcacgc agcacggccg tcagcagcgg
      541 ccgctcacca cggcgggtga gggcgcgcag gcgcgccagc agctcggtga aggcgaaggg
      601 cttaccgagg tagtcatcgg cgccggcatc cagtccctcg acccgctcgt ccagctcgcg
      661 ccgcgcgctc aggatcagca ccggcgtgtg gaccgcctcg cgccgcaggc gccggctcag
      721 ctcgagcccg tcgatgccgg ggagcatgcg gtcgagcacg atggcgtcgt agatgccggc
      781 cagcgccaat tcgagcccct gctcgccgtc gtgagcgata tccacctcgt agcgttcccg
      841 gcgcaggacg cgcgcgatca cgtccgccag gcccacctca tcttccacca ccaggatacg
      901 catggtcgcg ccggctacct tactagacag gcccgctggc tccccctccg ccgggcgcgc
      961 gtccccgccg cagcaggcga aaggcgagca ggaagatcac cacggcgacg ctcgccaggt
     1021 agagcagttg cgcccagagc cggtgcgcgt cgctgcccgc gccgagcccg tgcgccagcg
     1081 ctatcagcca gagcggaaac gcgccgtagt gcagcagccg ccagccccgt tgcccgatga
     1141 agcgccgcag cgcgaagctt gccgtaatga taatcatgcc atagccggtc aggatgccga
     1201 gatcggtcca gggctggcgc accgccgatt gcaggggcac gagcacgccc agcagcccga
     1261 gcggcacggt cgggtcgatc gccagcgaga gcagatggag cgccaggaag acaaaggcca
     1321 gctcggtggt gaagcggtgc gcgtcccagg cggcggcacg gccgcccagc ccgagcaggc
     1381 tggtcgtcag gcccaggccg gagaccaccg accaccagag cagcaggtag agcgtgaagc
     1441 cggaggcccg cgccaggtac caggtggccg ggctgggcga gaagccgagc agcagggcgg
     1501 cggccagcag ccccgcgatc cccagcgcga ccagcagcac gcccagcggc gcagccagcg
     1561 ggccgtgccg cgctgcgccg gcaggtttgg ccggtgattc aatcaccatc gatctcaacc
     1621 cctcacgtac gttacgcggc gcggcaccgt ggacgctcgg tggctgaccc cgcgtgagga
     1681 gagatccttc gcctgccctg agcttgtcga agggatgaca ggaaagcgac tcgcgcttcc
     1741 cctgtcagcc tgaggccgca gccgaaggat ctctccgccg ttccatgggg caccgcatgc
     1801 tgtgatcttc gtgctcggtg gcgccggtat cacgacgaac tggtgcggac cttgggcttg
     1861 gccttcttga ccggcgcggc cagccgcacc gtgcccgagg tctgctgccc ggcgctggcc
     1921 ccggcgctcg acggcaagac gctcgtcgcc ggtggcacgg cgccggccgc taccggcagg
     1981 gattgccggt cctgcaccac ggtcaggccg aagagcccca ggaagctgcc cagcgcgccg
     2041 gccaggatca gctttttgag ccgcgcgtgc cgctccatcg tcgccacccg gccgcgcggc
     2101 gccagcccgg cgcgcggcgc gcgcagcgcg tccagatcag tcgtggtcat cgtcgtgccc
     2161 atcgtgctca tcatgattat catgctcgtt
//
LOCUS       DASUBI010000272         6741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_509675, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000272 DASUBI010000000
VERSION     DASUBI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509675"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VFU72_05180"
     CDS             <1..1575
                     /locus_tag="VFU72_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422912.1"
                     /translation="PAATSAATKAATPASSGSAPGSPTAAAAAATKAPQGKQGGTLTY
                     GYNVQQLLQLDPARISTGRVAGELLPQLFSALVQFDEKLSIVPDLAEKWDISDDGLNY
                     TFHLRQGLKFHNGDTLTGKDFVYTYNRTLDPKLASPHANKLKTITKAESPDDLTFKLT
                     FSAPFAPFLATTCTRGPGRALTPVPQKAVEQIGEDQFLLKPVGCGPFMLQPETMNAQS
                     GFTMVAWDGWYGGRPLLDKIVVKFIPEPSSQVNALAAGDIDAMNQVPAQGYAQLEGND
                     KVSVLKVHGTNWIAVQFNTATAPFSNQDARMAIAKGIDRDQFIKTALFGLAEPSVGAI
                     APAFAWATQTEADLKDDPEKYDPAAAKQLAESSGLTKIKPELLAGASDHRAEDTLRNL
                     ILKIGVDINLVLLQDADYNNRWQNGQYQMAIQGSVVDADPDDNDYNFFYPDGPWNTGK
                     WNRDEAKKLLDDERAVTDQKKRAQDFQQLQDLARKEAAFAFLYHAYYLPGFQKYVKGY
                     RQIPEIFYAETIWLDK"
     gene            1666..2610
                     /locus_tag="VFU72_05185"
     CDS             1666..2610
                     /locus_tag="VFU72_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422913.1"
                     /translation="MTQYIIRRLIYSIFIIWGCTTVVFFMLRLVPGDPVAAMLGIEYT
                     PQAAAQLRKNLGLDQPIYVQYVKWIGELAHGDLGKSIDTQQSVLAQIKSAFPKTLSIT
                     ILGFLIGVVVAVPMGMLAALKRDSIFDYIASITTFIGISLPTFWFGIVFILIFAVQIR
                     LLPSVGYVPPSKGIWPWFEHLILPGLAAGVGEAAILMRFVRAGLLETMGSDYIRTARA
                     KGLPASRVIMGHAMRNSLIPVVTVAGLSLAGLLGGLVITETVFAISGIGRLLTVAIFA
                     KDYPIVQGVILVITLVFVLANLAVDIMYTFLDPRIRYG"
     gene            2603..3550
                     /locus_tag="VFU72_05190"
     CDS             2603..3550
                     /locus_tag="VFU72_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5422914.1"
                     /translation="MADEKSVERMATSATARASVDEQRLDQLAEQRRAEGSRFWRRVR
                     AEKKAFLGLIFFAVLVIISVGGPIIAPYGADDVSFEQLEKPTLHHPMGTDSFGHDLLS
                     RVILGTRVSFSVGIVGAILSMIAGVAAGVVSGYYGGWIDNLIMRVVDLIWAFPAIVLA
                     MGLVSIYGAGAKNIIIAIAISNLVAFARIVRGDVLSLRESDFTLAARSIGVRDGRIML
                     RHLLPNVAAPILVQMTLVVGFGILTETGLTFLGLGVNPATPTWGQALNDSRNFIHQAW
                     WLAVFPGLAIGFTVLSMNLLGDGLRDALDVRNVSEAQGT"
     gene            complement(3766..5466)
                     /locus_tag="VFU72_05195"
     CDS             complement(3766..5466)
                     /locus_tag="VFU72_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5422915.1"
                     /translation="MRGADVIVEMLKAYGVDYVFGVPGDTTMPLYDALYQARGAINHV
                     LARDERSASFMADAYARLTNRPGICEGPSGGGATYIVPGVAEANGSSIPVICFTSDNP
                     LRWEHTGALTAIDQVALLKPATKWNTAVKQADRLPDLIRRAFREATSGRPGAVHLSLP
                     EDVLETGVDNPRIYAEDACASFPSYRTRPSRAESETIYRALKAARQPMIVAGGGVTIA
                     QGGEELARLAALLEIPVGTTINGKGSMPEYSPWSVGVIGGNGGRPYANQLMREADTIL
                     YLGTKVNYLVTLNGSIPAPEVTVLQIDVDPNELGNNVRLSAGAAADAREALRDLVALA
                     EADSDRRLVREVGAAELARRAGEFWQAFQAKAADDSFPVKPQRIIAALEELLPERAVI
                     VADPGTMTPFTAANFRVHQSGRSVVIPRAHGGLGYALPASVGAACARPGERIVALIGD
                     GSFGMSGTELETVGRMNLPITFIQFSNGSFGWIKMLQKLYFSDRYHNVDFTSTVDYAG
                     AATALGVPGVRVEHADQLADVIAESLRAEGPLFIDVPTESELEETPPVHAWQQALGLV
                     "
     gene            5604..6491
                     /locus_tag="VFU72_05200"
     CDS             5604..6491
                     /locus_tag="VFU72_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422916.1"
                     /translation="MRAEDIRTIALPDGRELSFVEYGDPAGRPVLYFHGIPGSHRSPA
                     FADEPARVRGVRIIAVDRPGIGRSTFQPQRRFLDWPQDVAALSDTLGLERFAVAGVSG
                     GAAYVAACAYALPERISFAGIISGMGPLETPGATQGMRGMTSRRRIEIALARRAPRLT
                     CRLAQGHIARQSDPRRGDVVALMRDELAPADVELLGHPELAAAVRHDFAEAFRQGPRG
                     VTWDLVLYARPWGFSPTDIPIEVHLWHGDADVTVPIAFGRALAAAIPRCRTRWFPGQG
                     HLLAISYIDEMLDVIAARI"
     gene            6547..>6741
                     /locus_tag="VFU72_05205"
     CDS             6547..>6741
                     /locus_tag="VFU72_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422917.1"
                     /translation="MQAEELRALQSPLKTRYREDPGSALLTLRAEGTLDAAAITCAVG
                     THHGDVEAGLHPATGGSGLEA"
BASE COUNT         1068 a         2372 c         2212 g         1089 t
ORIGIN      
        1 ccggccgcga cgtcagccgc gacaaaagca gcaacgccag cgtcgtccgg cagcgcgccg
       61 ggatcgccca cggcggcagc cgcggcggcc accaaggcgc cgcaggggaa gcagggcggc
      121 acactcacct acggctacaa cgtccagcag ctcctgcagc tagacccggc ccgcatcagc
      181 accggccgcg tcgccggcga gctgctgccc cagctcttct ccgcgctggt gcagttcgac
      241 gagaaactga gcatcgtgcc ggacctcgcg gagaagtggg acatctcgga cgacggcctc
      301 aactacacct tccacctgcg tcaggggctg aagttccaca acggcgatac gctcaccggc
      361 aaagacttcg tctacaccta caaccgcacg ctcgacccga agctggcctc accacacgcc
      421 aacaagctca agaccattac caaagcagag tcgccggacg atctcacctt caagctcacc
      481 ttcagcgcgc ccttcgcgcc gttcctggcg accacctgca cccgggggcc gggccgcgcc
      541 ctgacgcccg tgccgcagaa ggccgtcgag cagatcggtg aagaccagtt cctgctcaag
      601 ccggtcggct gcgggccgtt catgctgcag ccggagacga tgaacgcgca gtccggcttt
      661 accatggtgg cctgggacgg ctggtacggc gggcgcccgc tgctcgacaa gatcgtggtc
      721 aagttcatcc cggagccgtc cagccaggtg aatgccctgg ccgccggcga tatcgacgcg
      781 atgaaccagg tgccggcgca gggctacgcc cagctcgagg gcaacgacaa ggtctctgtg
      841 ctcaaggtcc acggcaccaa ctggatcgcc gtgcagttca acacggccac ggcgccgttc
      901 agcaatcagg acgcgcgcat ggcgatcgcc aaggggatcg accgcgacca gttcatcaag
      961 accgcgctct tcggactggc cgagccgagt gtcggcgcga tcgccccggc ctttgcctgg
     1021 gctacccaga ccgaggcgga cctcaaggac gatccggaga agtacgaccc ggccgccgcc
     1081 aagcagctgg ccgaatcctc cggcctgacc aagatcaagc cggagctgct cgccggcgcc
     1141 agcgaccacc gcgccgagga cacgctgcgc aacctgatct tgaagatcgg ggtcgatatc
     1201 aatctcgtcc tgctgcaaga cgccgactac aacaaccgct ggcaaaacgg ccagtatcaa
     1261 atggccatcc aggggagcgt ggtggacgcc gacccggatg acaacgacta caacttcttc
     1321 tatccggatg gcccctggaa caccggcaaa tggaaccggg acgaggccaa gaaactcctt
     1381 gacgacgagc gcgcggtcac cgaccagaag aagcgcgccc aggacttcca gcagctgcag
     1441 gacctggccc gcaaggaggc cgcgttcgcg ttcctctacc acgcgtacta cctgccggga
     1501 ttccaaaagt acgtgaaggg ctaccgccag atcccggaga tcttctacgc cgaaaccatc
     1561 tggctggata agtagcgcgc gccggagtga gccggtgatg ggaggcgggt ctcaacggcc
     1621 cgcctcccgc tccccggcgc agggtaccaa ccagaggggc gtcccatgac gcagtacatc
     1681 attcgccgct tgatctactc gattttcatc atctggggct gcacgacagt cgtcttcttc
     1741 atgctgcgcc tggttcccgg cgacccggtg gcggccatgc tcggcattga gtacacgccc
     1801 caggccgcgg cccagctgcg caagaacctc ggcctcgacc agcccatcta cgtccaatac
     1861 gtcaagtgga tcggcgagtt ggcgcacggt gacctgggca agtcgatcga cacccagcaa
     1921 tcggtactgg cccagatcaa gagcgcgttc cccaagacgc tttcgatcac gatcctcggg
     1981 ttcctcatcg gggtggtcgt ggcggtgccg atggggatgc tggccgcgct caaacgcgac
     2041 agcatcttcg actacattgc cagtatcacg accttcatcg gcatcagcct gccgaccttc
     2101 tggttcggca tcgtcttcat cctgatcttc gcggtgcaga tccgcctgct gccctcggtg
     2161 ggctacgtcc ctccttccaa gggcatctgg ccctggttcg agcacctgat cctgcccgga
     2221 ttggccgccg gcgtcggtga ggcagccatc ctgatgcgct tcgtgcgcgc cggactcctg
     2281 gagacgatgg ggagcgatta catccgtacc gcgcgcgcga agggcctgcc ggcgagccgc
     2341 gtcatcatgg ggcacgcgat gcgcaactcg ctgatcccgg tcgtcaccgt cgcggggctc
     2401 tcgctggccg ggttgctggg cggcctggtc atcaccgaga cggtcttcgc catcagcggc
     2461 atcggccggc tgctgacggt ggcgatcttc gccaaggact acccgatcgt gcagggcgtg
     2521 atcttggtga ttaccctggt cttcgtacta gcgaatctgg cggtcgatat catgtatacg
     2581 ttcttggatc caaggatacg ctatggctga tgagaagtcg gttgaacgca tggccacgtc
     2641 cgctaccgcg cgcgcgtccg tcgacgagca gcggctcgat caactggcgg agcagcgaag
     2701 ggcggagggt agccgcttct ggcgccgggt gagggccgag aagaaggcct tcctggggct
     2761 gatcttcttc gctgtgctgg tcatcatctc cgtgggcggg ccgataatcg ccccgtacgg
     2821 agccgatgac gtcagcttcg agcagttgga gaagccgacc ctgcaccatc cgatgggcac
     2881 cgattcgttc ggtcacgacc tcctcagccg ggtcatcctc ggcacgcgcg tctcgttcag
     2941 cgtcggcatc gtcggcgcga tcctgtcgat gatcgccggc gttgcggctg gtgtggtctc
     3001 cggctactac ggcggctgga tcgataacct gatcatgcgc gtcgtcgatc tgatctgggc
     3061 atttcccgcc attgtgctgg caatggggct ggtttccatc tacggggcgg gcgccaagaa
     3121 catcatcatc gcgatcgcga tctcgaacct ggtcgccttc gcgcgcatcg tgcgcggcga
     3181 cgtgctcagc ctgcgggagt ccgacttcac cctggcggcc cgctcgatcg gcgtgcgcga
     3241 cggccggatc atgctgaggc acctgctgcc gaatgtggcc gcgccgatcc tggtacagat
     3301 gacgctggtc gtcggcttcg gcattttgac cgagaccggg ctgaccttcc tcggcctggg
     3361 cgtcaacccg gccacgccga cctggggcca ggcgctcaac gacagccgca acttcatcca
     3421 tcaggcctgg tggctggcgg tcttccccgg cctggcgatc ggcttcaccg tgctcagcat
     3481 gaacctgctc ggcgacggcc tgcgcgacgc gctcgacgtg cgcaacgtca gcgaggccca
     3541 gggcacgtaa cgagtgacgg gcggtcacct ggcgcccggg tgaggttcct tcgacgcgcg
     3601 tagatgacca tggcgaattc ggggcccggc gaacggcaca gatcgtcgcc ggcggtccgt
     3661 catccccagt tatgtcatcc cgcgcgcagt cgaaggatcc gtcctgacgc gggaccgatg
     3721 ccgcacgatc gccgcgcaac gcccccgcac gcacagggcc ggacgctata ccagccccag
     3781 cgcctgctgc caggcgtgca ccggcggcgt ctcctccagc tccgactcgg tcggcacgtc
     3841 gatgaagagc ggaccttcgg cccgcaggct ctcggcgatc acatcggcga gctgatcggc
     3901 gtgctccacg cgcacccccg gcaccccgag cgccgtcgcc gctcccgcgt agtcgaccgt
     3961 gctggtgaag tcgacattgt ggtagcggtc gctgaagtag agcttctgca gcatcttgat
     4021 ccagccgaag ctgccgttgc tgaactggat gaaggtaatc ggcaagttca tgcgccctac
     4081 cgtctccagc tcggtgcccg acatgccgaa gctgccgtcg ccgatcaacg ccacgatacg
     4141 ttcgccgggg cgggcgcagg ccgctcccac cgaggccggc agcgcgtagc ccagcccgcc
     4201 gtgggcgcgc gggatgacca cgctgcgccc gctctggtgc acgcggaagt tggcggcggt
     4261 gaagggcgtc atggtgccgg ggtcggccac gatcaccgcg cgctccggca gcagttcctc
     4321 cagcgccgcg atgatgcgct gcggcttgac cgggaagctg tcgtcggcgg ccttggcctg
     4381 gaaagcctgc cagaactcgc ccgcgcgccg cgccagctcc gccgcgccca cctcgcgcac
     4441 cagccggcgg tcgctgtccg cctcggccag cgccaccagg tcgcgcagcg cctcgcgcgc
     4501 gtcggcggcc gcgcccgcgc tcaggcgcac gttgttgccc agctcgttcg gatcgacgtc
     4561 gatctggagc acggtgacct cgggcgccgg gatgctgccg ttcagcgtca ccaggtagtt
     4621 caccttggtg ccgaggtaga ggatggtgtc ggcctcacgc atcaactggt tggcataggg
     4681 acggccgccg ttgccgccga tcacgcctac gctccagggg ctgtactccg gcatgctccc
     4741 cttgccgttg atcgtggtgc cgaccgggat ctccaacagc gcggccagcc gggccagctc
     4801 ctctccgccc tgcgcgatcg tcaccccgcc gccggccacg atcatcggct ggcgcgccgc
     4861 cttcagcgcc cggtagatcg tctcgctctc cgcgcgactt ggccgcgtgc ggtagctggg
     4921 gaagctggcg caggcgtcct cggcgtagat gcgcgggttg tcgacgccgg tctccagcac
     4981 atcctccggc agcgagaggt gcacggcgcc cggacggccg ctggtcgcct cacggaaggc
     5041 gcggcggatc aggtcgggca gccggtcggc ctgcttgacg gcggtgttcc acttggtggc
     5101 gggcttgagc agcgcgacct gatcgatggc ggtcagcgcg ccggtgtgct cccagcgcag
     5161 cgggttgtcc gaggtgaagc agatcaccgg gatcgaggag ccgttggcct cggccacgcc
     5221 gggcacgatg taggtcgcgc cgccgccgct cggtccctcg cagatgccgg gtcgattggt
     5281 gagccgggcg taggcgtcgg ccatgaagct ggccgagcgc tcgtcacgtg ccagcacgtg
     5341 gttgatcgcg ccgcgcgcct ggtagagcgc gtcgtagagc ggcatggtgg tgtcgccggg
     5401 gacaccgaag acgtagtcga ccccgtaggc cttcagcatc tcgacaatga cgtccgcccc
     5461 gcgcatcact cgctcccctc atcgaacacc gacggctgga gtcgccccga ggcgacgcca
     5521 gttggtaggc tacgacgagc ggcgccccgc cgtccagagc gggacatcgg taagcattcg
     5581 tagctgggaa cgagcgtgag cgcatgcgcg ccgaggatat ccgaaccatc gcgctccccg
     5641 acgggcgcga gctgagcttc gtcgagtacg gcgatcccgc tgggcggccg gtcctctact
     5701 tccacggcat ccccggctcg caccgctcgc ccgccttcgc ggacgaaccg gcgcgggtgc
     5761 gcggcgtgcg catcatcgcc gtcgaccgcc ccggcattgg gcgctcgacc tttcagcccc
     5821 aacgccgctt cctcgactgg ccccaggatg tcgccgcgct gagcgacacg ctggggttgg
     5881 agcggttcgc ggtggccggc gtctcgggcg gcgcggccta cgtggccgcc tgcgcctatg
     5941 cgcttcccga gcggatcagc ttcgccggca tcatcagcgg catggggccg ctggagacgc
     6001 cgggcgcgac ccagggcatg cgtggcatga ccagccgccg gcgcatcgag atcgcgctgg
     6061 cccgccgcgc accacgcctg acctgccgcc tggcgcaggg ccacatcgcc cggcagtccg
     6121 acccccgtcg cggcgacgtg gtcgccctga tgcgcgacga gctggcgccg gccgacgtcg
     6181 agctgcttgg gcacccagag ctggcggcgg cggtgcgcca tgacttcgcc gaggcctttc
     6241 gccagggccc gcgcggtgtg acctgggatc tcgtgctcta cgcccgcccc tggggtttct
     6301 ctcccaccga cattcccatc gaggtccatc tctggcacgg cgacgccgac gtgaccgtgc
     6361 cgatcgcctt cggccgcgcg ctggccgccg ccatcccgcg ctgccggacg cgctggttcc
     6421 ccggccaggg gcacctgctg gcaatcagct acattgacga gatgctcgac gtcatcgccg
     6481 cgcgcatctg agcggctgcc tgctaccatt cgccgggcga gccggcctga ctgaaggagc
     6541 gtatcgatgc aagctgagga attgcgggcg ctacagtcgc ccttgaagac gcgctaccgc
     6601 gaggatcccg ggtcggcgct gctgactctg cgcgccgaag gcacgctcga cgccgcagcc
     6661 atcacctgcg ccgtcggcac gcaccacggc gacgtcgagg ccgggctgca cccggcgacc
     6721 ggcggcagcg gcctggaggc c
//
LOCUS       DASUBI010000273         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1085568, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000273 DASUBI010000000
VERSION     DASUBI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085568"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFU72_05210"
     CDS             <1..666
                     /locus_tag="VFU72_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5422918.1"
                     /translation="SILRDMGAHITGAGTPHIRVQGVDRLRGYRAAILPDRIEAGTIA
                     IAAAISNGEVVLDHVSEPDMLPLTSKLREAGAEVWWSGDSMLVRGSGRLEATEIQALP
                     FPGFPTDLQAAFAVLMTQAHGTSQIYERVFNDRLRYVEELCRMGADICLKDKQHAVIE
                     GPTALHGSVVSALDIRSGASLVLAGLVAEGETQILETQHLRRGYEDIVGKFARLGADI
                     SYV"
     gene            792..1406
                     /locus_tag="VFU72_05215"
     CDS             792..1406
                     /locus_tag="VFU72_05215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5422919.1"
                     /translation="MLVSPRRRIAALSAGTIRSRLIPALAVTLVVAMLALLGWAMFAP
                     KSETVSQDGRIDAPGVFVPIKGRTAPNFDLVDFSGQNISLKDFRGKTVVLNFWASWCD
                     SCKAEAPLLSHVAPTLDSNKTVILGIDALDARADGQQFMQQYGLTFRSAFDANGSVAV
                     DYGISGMPETFIISPSGKLVGKFFGAITKPEQVSEAVAAAEAAG"
     gene            1419..2144
                     /locus_tag="VFU72_05220"
     CDS             1419..2144
                     /locus_tag="VFU72_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5422920.1"
                     /translation="MTDLSLFAAFVAGLASFLSPCVCPLVPGYLSLISGNSLSAEGER
                     RAGWRVAGSSLVFVAGFSLVFVTLGVTASSLGTAITAHRDTLNRLAGVFMIVMGLFIV
                     GALQVPWLYRERRFHPEPRHSLTRSETLLLGMAFGFGWTPCIGPLLGSILVYTSAAAN
                     VSRGTLLLTSYSIGLGVPFIVVGVGLGRALGAIRWISRHYQVISIPSGATMMAVGLLF
                     LTGRFFYFSISAQRLVEQLGQVL"
     gene            2224..>2481
                     /locus_tag="VFU72_05225"
     CDS             2224..>2481
                     /locus_tag="VFU72_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopD"
                     /protein_id="HEU5422921.1"
                     /translation="MTAIEILRLLLRLAHALAAALWLGGGAYYALALRPQLRDADPAR
                     QALAREAQRAFGEWAATLTLVMIVTGVILLYDRLTDGQGTLV"
BASE COUNT          345 a          815 c          854 g          467 t
ORIGIN      
        1 tcgatcctgc gcgacatggg cgcgcacatc accggcgcgg gtacgccgca catccgcgtg
       61 cagggggtcg atcgcctgcg cggctatcgc gccgccatcc tgcctgaccg tatcgaagcc
      121 ggcaccatcg ctatcgcggc cgccatctcc aatggcgaag tggtgctcga tcacgtttcc
      181 gaaccggata tgctgccctt gacctcgaag ctccgcgagg ccggcgccga ggtatggtgg
      241 agtggcgact cgatgctcgt gcgcggcagc gggcggctgg aggcgaccga gatccaggcg
      301 ctccccttcc ccggcttccc caccgacctg caggccgcgt tcgcggtgct gatgacccag
      361 gcgcacggca ccagccagat ctacgagcgc gtcttcaacg accggctgcg ctacgtcgag
      421 gagctgtgcc gcatgggagc cgacatctgc ctgaaggaca agcagcacgc ggtgatcgaa
      481 ggcccaacgg cgctgcacgg ctcggtcgtc agcgcgctcg acatccgcag tggggccagt
      541 ttggtgctgg cgggtctggt ggccgagggg gagacgcaga tcctggagac gcagcacctg
      601 cgccgcgggt atgaggatat tgtcgggaaa ttcgcccgcc ttggcgctga catttcttac
      661 gtgtgatcgt cgccaatagg ggaatccttc ggcatccccg gcggttggct ttgactttgc
      721 cggttgggtc gccataatag gctgcggtag acgtcagttg tccggccgag cgtggcagtt
      781 gggaagggcc aatgctggtc agcccgagac gacgaatagc ggctctctcc gcgggcacga
      841 ttcgctcgcg gctcatcccg gccctggccg tgacgctggt ggtagcgatg ctggcgctgc
      901 tcggctgggc catgttcgcg cccaagtcag agaccgtcag ccaggacggc cgcatcgacg
      961 cgccgggtgt cttcgtgccg atcaagggcc gcacggcgcc taacttcgac ttggtcgatt
     1021 tctcgggcca gaacatctcg ctcaaggatt tccgtggcaa gaccgtggtg ctcaacttct
     1081 gggcctcctg gtgcgactcc tgcaaggccg aggctccgct cctgtcgcac gtcgcgccga
     1141 cgctcgattc gaacaagacc gtgatcctgg ggatcgacgc gctcgacgcc agggccgatg
     1201 ggcagcagtt catgcaacag tatgggctta ccttccgctc ggccttcgac gccaacggct
     1261 cggtcgcggt cgactacggc atctcgggga tgcccgagac cttcatcatc agtccgagcg
     1321 gcaagctcgt gggcaagttc ttcggcgcga tcaccaagcc ggagcaggtg agcgaggccg
     1381 tcgccgccgc cgaggccgcg ggctagtgag ccggcgcagt gactgacctg agcctcttcg
     1441 ccgccttcgt cgccgggctg gcgtcgtttc tctcgccctg cgtctgtccg ctggtgcccg
     1501 gctacctctc gctgatctcg ggcaacagcc tgagcgccga gggcgagcgc cgggccggct
     1561 ggcgtgtggc cggttcgagc ctggtcttcg tggccggttt ctcgttggtt ttcgttaccc
     1621 tcggcgtgac ggcctcctcg ctcggcacgg ccatcacggc gcaccgcgac acgctcaacc
     1681 gcctggccgg cgtcttcatg atcgtgatgg gtctcttcat cgtcggcgcg ctgcaggtgc
     1741 cctggttgta ccgtgagcgg cgctttcacc cggaaccgcg ccattcactg acgcgctcgg
     1801 agacgctgct gctgggcatg gccttcggtt tcggttggac gccctgcatt gggccgctgc
     1861 tcggatcgat cctggtctac accagcgccg cggccaacgt cagccgcggc acgctgctgc
     1921 tgacgtcgta ctcgatcggg ctgggcgtgc ccttcatcgt ggtgggggtt ggactggggc
     1981 gggcgctggg cgcgatccgc tggatctcgc gccactatca ggtcatctcg atcccgagcg
     2041 gagcgaccat gatggcggtg gggctgctct ttctgacggg ccgattcttc tatttcagta
     2101 tcagcgccca gcggcttgtc gagcaattgg gacaggtcct atgaggacat ttcccgacgt
     2161 tcgcgcgcgc gcggcgtctg gtacagttcg cgcgacgagc ggccggcggg ggcaggccgg
     2221 cgtgtgaccg ccatcgagat cctgcggctg ctgctgcgcc tggcccacgc gctggcggcg
     2281 gcgctctggc ttggcggcgg cgcctactac gcgctggcgc tgcgtccgca actgcgggac
     2341 gccgatccgg ccaggcaggc gctagcgcgc gaggcgcagc gagctttcgg tgagtgggcc
     2401 gcgacgctga cgctggtcat gatcgtcacc ggcgtgatct tgctgtacga ccgcctgacg
     2461 gatggacagg gcacgctggt c
//
LOCUS       DASUBI010000274         5051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_621360, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000274 DASUBI010000000
VERSION     DASUBI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621360"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..995)
                     /locus_tag="VFU72_05230"
     CDS             complement(51..995)
                     /locus_tag="VFU72_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422922.1"
                     /translation="MSVRSGYIELDDGRLAYDEAGNGYPLVLLHGFTVDRQVWDRQFL
                     PLTRTRRVIRYDLRGFGASSVPGETPYSHVDDLAHLLDELGLDRADLIGLSLGGGVAV
                     DFALAHPDRVRGLVLVDPTLGGVPWSVELLDEFRALNHTAREQGVDAARARWLRHPFF
                     AAALEHPGAAALLNEIEARYSGWHWLHRDPARALRPPAVERLAELQAPTLVIVGERDV
                     PDIRRMAELLAKGIPGASQIVMPGAGHLPNVEVPKEFNRVLAGFLDRLDAGEASDGGE
                     RRSWVPRQDGSFDCAQDDRGKGEADDEDRRRTRMRLVR"
     gene            1193..2149
                     /locus_tag="VFU72_05235"
     CDS             1193..2149
                     /locus_tag="VFU72_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5422923.1"
                     /translation="MRIGVMIEGQEAITWERWFHILDVADRLGYDSLWRSDHLFSVMG
                     MSQRPTLALWPSLTAVGLKSQRLEFGQLVSPTTFRNPVMLAKEAVALDHLSNGRYWLG
                     LGAGWNEREHAAFGFDLPPLKDRMDRLEEALEVIALLWTGEPVSYAGRFFHLRDAQMQ
                     PTPLKQGGVPLIIGGSGEKRLLALVARFADEWNCTVMPPERYAHKAQVLAEHCERVGR
                     DPKTIARSIMLGHIVGRDQAELEQRAAWLGNFLPALKDRSPGEIVQNRREGGWLVGTP
                     NEAVAQLQQLSQLGVSRVMLQTFNLEDDAELELIAREVMPQV"
     gene            complement(2300..3145)
                     /locus_tag="VFU72_05240"
     CDS             complement(2300..3145)
                     /locus_tag="VFU72_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5422924.1"
                     /translation="MPERPVVDLHSHSDASDGMLPPGDLIARAAELGLRVLALTDHDT
                     TAGLAAAVAAGARLGVAVIRGLELGISVSQGELHMLGYFIDPAAPALQATLTMLREGR
                     RDRAGVMVRQLNQAGVPVTLDEVERLAGGEVVGRAHVARALVERGIAGSMNEAFDRFL
                     AAGKPGYAPRPRLSAEEAIDVIHAAGGAAVLAHPFSVADLAETLPRLAAHGLDGLEVF
                     YAAYDDLARARLAVLAAEHGLAPTGGSDFHGIPGPGEEGRALASAPVPLEAVTLLRQR
                     AEKHQ"
     gene            complement(3236..3511)
                     /locus_tag="VFU72_05245"
     CDS             complement(3236..3511)
                     /locus_tag="VFU72_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEU5422925.1"
                     /translation="MEERRGDVLKVSARSRPSAVAGAIAGVVRESGRAEVQAIGAGAT
                     NQAVKAVAIARDYLHETGIEAVCLPAFIDVQIDEESRTAIRLIVEPR"
     gene            complement(4022..>5051)
                     /gene="rny"
                     /locus_tag="VFU72_05250"
     CDS             complement(4022..>5051)
                     /gene="rny"
                     /locus_tag="VFU72_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5422926.1"
                     /translation="EQEAKQEANVRSQRILATTIQRIASDYVAESTVSVVPLPSDDMK
                     GRIIGREGRNIRALEQATGVDLIVDDTPEAVTLSSFDPVRREIARRALQKLIQDGRIH
                     PARIEEIVNKTRQEVEQIIREEGEKVAYEANVQGLHPDLIKLLGRLKYRTSYGQNVLQ
                     HSLEVSQIAGALAAELGAEYNVCKTAALLHDIGKAVDHEVEGPHALIGADIARRLGRS
                     AKIVHAIAAHHGEEEPQTVEAFIVATSDAISGARPGARREMIETYIKRLEALEGVANS
                     FAGVEKAYAIQAGREVRILVQPDKVDDLAAQRLARDIVKKIQETLEYPGQIKVTVIRE
                     TRAIDYAR"
BASE COUNT          782 a         1780 c         1708 g          781 t
ORIGIN      
        1 cagagcctgc cctgagcctg ccgaagggat gacaggctat aggggatgac ctaccggaca
       61 aggcgcatcc tcgtccgacg ccgatcttcg tcgtccgcct ctcccttgcc cctgtcatcc
      121 tgagcgcagt cgaaggatcc gtcctgacgc ggcacccagc tccgtcgttc gccaccatcc
      181 gacgcctcac ccgcgtccag gcggtcgagg aagccagcca gcacgcggtt gaactccttc
      241 ggaacctcga cattcggcag gtgcccggcg ccgggcatca cgatctgaga ggcgccaggg
      301 atgcccttcg ccagcagctc agccatgcgc cgaatgtccg gcacgtcgcg ctcgcccacg
      361 atcaccagcg tgggcgcctg aagctcggcc aggcgctcga cggccggagg ccggagcgcg
      421 cgggccgggt cgcggtgcag ccagtgccag ccggagtagc gcgcctcgat ctcattgagc
      481 agcgcggccg cgccgggatg ctccagcgcc gccgcgaaga aggggtggcg cagccagcgc
      541 gcccgggccg cgtcgacgcc ctgctcgcgc gccgtgtgat tgagcgcgcg gaactcgtcc
      601 agcagctcca ccgaccaggg cacgccgccg agggtcggat cgaccagcac gaggccgcgc
      661 acgcgatcgg ggtgcgccag cgcgaagtcg acggctacgc ccccgcccag cgagagcccg
      721 atcagatcgg cccggtcgag tcccagttca tcgagcaggt gagccaggtc gtcgacgtga
      781 gagtacggcg tctcaccggg gaccgaagag gcgccgaagc cgcgcaagtc gtagcggatc
      841 acacgacgcg tgcgggtcag cggcaggaat tgccggtccc agacctgccg gtcgacggtg
      901 aagccgtgca gaagcaccag cgggtagccg ttgccggctt cgtcgtaggc cagccgccca
      961 tcgtccagct cgatgtaccc gctcctgacc gacacggcgc acgactccca cgaaatgacg
     1021 atccatcgtc gttcatcata gatggcggca ggcggcggtc aaggttggag attcgcggcc
     1081 catcggcgac aatccgcgcg ttggggagcc ctcgctcccg gcccctgacc caggtgcgcc
     1141 gggtgagggg agtcgacgtg tatggaacaa ccgggcaatt gaaggagcga gcatgcgcat
     1201 tggggtgatg atcgaggggc aagaggcgat cacctgggag cgctggtttc acattctcga
     1261 cgtggccgac cgcctgggtt acgactcgct ctggcgctcc gatcacctct tctcggtcat
     1321 gggtatgagc cagcgcccga cgctggcgct ctggccctcg ctcacggcgg tcggcctcaa
     1381 gagccagcgg ctggagttcg gccagctcgt ctcgcccacc accttccgca acccggtgat
     1441 gctggccaag gaggcggtcg cgctcgacca cctctccaac ggccgctatt ggctcggcct
     1501 gggcgccggc tggaacgagc gtgagcacgc cgccttcggc ttcgacctgc cgccgctcaa
     1561 ggaccgcatg gaccggctgg aggaggcgct ggaggtcatc gcgctcctct ggaccggcga
     1621 gccggtcagc tacgccgggc gcttctttca cctgcgcgac gctcagatgc agcccacccc
     1681 gctgaagcag ggcggcgtgc ccttgatcat cggcggctcg ggagagaagc gactgctcgc
     1741 cctggtggcg cgcttcgccg acgagtggaa ctgcaccgtc atgccgcccg agcgctacgc
     1801 gcacaaggcc caggtgctgg cagagcactg cgagcgggtc ggccgcgatc ctaagaccat
     1861 cgcgcgctcg atcatgctcg gccacatcgt cgggcgcgat caggcggagc tggagcagcg
     1921 cgcggcctgg ctgggcaact tcctgccggc gctcaaggac cgctcgccgg gggagatcgt
     1981 gcaaaaccgc cgcgagggcg gctggctggt gggcaccccg aacgaggcgg tagcgcaact
     2041 ccagcagctc agccagctcg gcgtctcgcg cgtcatgctc cagaccttca acctggaaga
     2101 cgacgccgag ctggagttga tcgcgcgcga ggtcatgccg caggtgtagg cgggccgctc
     2161 agggggccct catccccagc cacggagagc cctcatcccc cgcccttctc ccgatgcgcg
     2221 ggagaaggga gacgaagagg tgtcgctgcc gacgcgccct gtcgcacggg gcagaaatcc
     2281 acgacgaaga atcgccgcgc tattggtgct tctcggcgcg ctggcgcagg agcgtgacag
     2341 cctctaaggg gaccggggcg ctggcgagcg cgcgaccctc ctcgcccggg ccggggatgc
     2401 cgtggaagtc gctgccgccg gtcggcgcca gcccgtgctc ggccgccagc accgccagcc
     2461 gtgcccgcgc caggtcgtcg taggcggcgt agaagacctc caggccgtcc agaccgtgcg
     2521 ccgccagccg aggcagggtc tcggccaggt cggccacact gaaggggtgc gccagcaccg
     2581 ccgcgccgcc cgcggcgtgg atcacgtcga tcgcctcctc ggcgctgaga cgcgggcgcg
     2641 gcgcgtagcc gggcttgccc gccgccagaa agcggtcgaa ggcctcgttc atcgagccag
     2701 cgatgccgcg ctcgaccagc gcgcgggcca cgtgcgccct gcccaccacc tcgccaccgg
     2761 ccagccgctc gacctcatcc aacgtcacgg gcacgccggc ctggttgagc tgccgcacca
     2821 tgaccccggc gcggtcgcgc cgcccctcgc gcagcatggt cagcgtggct tgcagcgccg
     2881 gcgcggcggg atcgatgaag tagccgagca tgtgcagctc gccctggctg acgctgatgc
     2941 ccagctcgag cccgcgaatc accgcgacgc ccagccgcgc gccggccgcg accgccgcgg
     3001 ccagtccggc ggtggtgtcg tggtcggtca gcgccagcac ccgcagcccc agctccgctg
     3061 cccgcgcgat caggtcgccc ggtgggagca tgccgtccga ggcgtcgcta tggctgtgga
     3121 gatcgacgac ggggcgctcc ggcatcgaca cgtcccttcg cgaggagtgg ggcaaaaaaa
     3181 cgtgcgccgc tggggtggcg gcgcacgcaa tataccaata tagatggtgc ggtgcttagc
     3241 gcggctcgac gatcagtcga atcgcggtgc ggctctcctc atcgatctga acgtcgatga
     3301 aggccgggag gcacacggcc tcgatgccgg tctcgtgcag atagtcgcgc gcgatggcca
     3361 cggccttgac ggcctggttg gtggcgccag cgccgatggc ctgaacctcg gcccgaccgg
     3421 actcgcgcac gacgccggcg atcgcaccgg ccaccgcgct cgggcgcgag cgggcggaga
     3481 ccttcagcac gtcgccgcgg cgctcttcga tcagcacggg agcctcgtcg gccgtggtgt
     3541 cgacgaaccc attcgtctcc tcgacctcat agccggtctc atcgggcgga aagctggcgt
     3601 tcggctcgat cttgatgtcg cgcagatcca gcttgctaaa gaaacgatcc atcatgtcct
     3661 ccatgagaac ggcacccgcc gctcccccat gtgcatggtg catgcagtgc gtcggtgttg
     3721 ttcccccacg tggtctgtcg tgtctcgcct cgtcaacctc ccaacccgcg tagaacgatg
     3781 acgggacggc cgccggccgc cggctgcgca ggcgttgcct gcgccgcgcg gactgtcccc
     3841 acacaggccg cgcgggcgcg cccaccgcac cagatattgg cgctccggcg ccaccggccg
     3901 aatcctcgca aggcttacca cagggttcaa gagtgcgtca acttatcagc caaccacgga
     3961 cccatactga aaacgaaacg ccggatcggc aataccgatc cggcgtccct ctgcaagggg
     4021 tctagcgtgc gtagtcgatc gcgcgggtct cgcggatcac ggtcaccttg atctgccccg
     4081 gatactccag ggtctcctgg atcttcttga cgatgtcgcg cgccagccgc tgcgcggcca
     4141 gatcgtcgac cttgtccggc tgtaccagga tgcgaacctc gcgtccggct tgaatagcat
     4201 aggccttctc gacgccggcg aaggagttag ccaccccctc cagcgcctcc agccgcttga
     4261 tgtaggtctc gatcatctcg cgccgcgcac ccggccgcgc gccgctgatg gcgtcggagg
     4321 tggcgacgat gaaggcttcg accgtctgcg gctcctcctc accgtggtgc gccgcgatgg
     4381 cgtgcacgat cttggccgag cggccgaggc gacgggcgat atccgcgccg atcagcgcgt
     4441 gcggcccctc gacctcgtga tcgaccgcct tgccgatgtc gtgcagcagc gcggcggtct
     4501 tgcagacgtt gtactcggcg cccaactccg ctgccagcgc gcccgcgatc tgtgagacct
     4561 cgagcgagtg ctgcagaacg ttctggccgt agctggtgcg gtacttgagc cggccgagga
     4621 gcttgatcag gtcggggtgg agcccctgca cgtttgcctc gtaggcgacc ttctcccctt
     4681 cctcacggat gatctgctcg acttcctggc gcgtcttgtt gacgatctct tcgatgcgcg
     4741 ccgggtggat acgcccgtct tggatcagct tctgcagcgc gcgccgcgcg atctcacgcc
     4801 ggacgggatc gaatgatgag agcgtgacgg cttccggggt gtcgtcgacg atcagatcga
     4861 cgccggtcgc ctgctccagc gcgcggatat tgcgaccctc gcgcccgatg atgcgcccct
     4921 tcatgtcatc gctggggagc ggcaccaccg agacggtgct ctcggccacg tagtcggatg
     4981 cgatgcgctg gatggtggtg gccaggatac gctgggagcg gacgttggcc tcctgcttgg
     5041 cttcctgctc g
//
LOCUS       DASUBI010000275         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1012030, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000275 DASUBI010000000
VERSION     DASUBI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012030"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU72_05255"
     CDS             complement(<1..201)
                     /locus_tag="VFU72_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEU5422927.1"
                     /translation="MPTDERLLPPEEALEIILRQARPLPDEIVPLARAGARVLAEPLI
                     AHQDLPPFRAATMDGFAVIAGDV"
     gene            357..2585
                     /locus_tag="VFU72_05260"
     CDS             357..2585
                     /locus_tag="VFU72_05260"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5422928.1"
                     /translation="MVTPRPDEERRPALVAATTPLPRAASWSDLEAVIQSRMPGMDLT
                     LVRHAYDVADLAHAGKLRRSGEPYICHPTEVAIILAEMQMDPETLAAALLHDVIEDTE
                     LTREDLAAEFGERVARLVDGVTKLGQIRWTPDEDQATREKNRQAESLRKMFLAMIDDV
                     SVVLIKLADRLHNMRTLEHMPHAKQMRSAQETLEIYAPLANRLGIWQMKSELEDLALR
                     YVDAQTYFAVERALDQRGIDRERFLNQVIAELHEALAEAGIRADISGREKHITSIVRK
                     MERKGRSFEEIYDVLGVRVIVDEKKDCYGALGVIHTMWHPIPGEFDDYIATPKEGVYQ
                     SIHTAVMGPDGHPVEIQIRTHEMHHIAEFGIAAHWRYKEGARPDPNVEAKISWLRQLM
                     DWRDEVVDAHEFVESLKSDVFQEMIYVFTPRGDIIELPAGATPVDFAYRIHTEVGHQC
                     VGAKVGGHLVPLNTRLQNGQVVQIMTSKTKVGPSRDWLLSSSGYITTASAREKVRQWF
                     RRQEREENIQQGRDLLEREMRRLGVEAKLDDTLKHFPHYQKLDDFLAAIGYGAVTPQQ
                     IATRLAEQEDRTVLAQTAPSTPALPPSLRVTGVGDLYTRLATCCKPVYGDQIIGYVTR
                     GRGITVHRADCYNVRRVAEPERLVQVSWGAEQKRLYPVSVRLEAWDRVGLLRDITTLV
                     ADAKMNMQSVLTSVNDDRTVTVLMTLEVESVRQLSQILQKLEAVRDVYDVRRDATPNM
                     SR"
BASE COUNT          514 a          935 c          901 g          398 t
ORIGIN      
        1 gacatcgccg gcgatgaccg cgaagccgtc catggtcgcg gcccggaagg gcggcagatc
       61 ctggtgggcg atcagcggct cggccagcac gcgcgcgccg gcccgcgcca gcggcacgat
      121 ctcatccggc agcgggcgag cctggcgcag gatgatctcc agcgcttcct cgggtggcaa
      181 caggcgctca tcggtcggca tggcaggcgc ggctcctcgt gttctccgga cgtggcaacg
      241 accccggcga cgatcttagc acagggcgaa cgcccgtcgc gttttgacaa tttcaccgct
      301 cacgactatg atttgttggg agtgctgtca cccttcactt aatggaatcc aggaacgtgg
      361 taaccccaag accggacgag gaacgacgcc ccgcgctggt ggcagcgacg acgccgctgc
      421 cgcgtgcggc aagctggagc gatctggaag cggtgatcca gagccggatg cccggcatgg
      481 atcttacgct ggtcaggcat gcctatgacg tcgccgacct ggcgcacgcc ggcaagctac
      541 gccgctcagg cgagccctac atctgccacc ccaccgaggt cgcgatcatc ctggccgaga
      601 tgcagatgga cccggagacg ctggccgccg ccctcctcca cgacgtcatc gaggataccg
      661 agctaacccg cgaggatctg gcggccgagt tcggcgagcg cgtcgcgcgg ctggtcgacg
      721 gcgtaaccaa gctggggcag atccgctgga cgcccgacga ggaccaggcg acccgcgaaa
      781 agaaccgcca ggccgagagc ctgcgcaaga tgttcctggc catgatcgac gatgtctcgg
      841 tcgtgctgat caagctggcc gaccggctgc acaacatgcg cacgctggag cacatgcccc
      901 acgccaagca gatgcgcagc gcgcaggaga cgctggagat ctacgcgccc ctggccaacc
      961 ggctcggcat ctggcagatg aagtccgagc tggaggacct ggcgctgcgc tacgtcgatg
     1021 cccagaccta cttcgcggtc gagcgggcgc tcgaccagcg cggcatcgac cgcgagcgct
     1081 tcctcaacca ggtgatcgcc gagctgcacg aggcgctggc cgaggccggc atccgggccg
     1141 atatctccgg ccgcgagaag cacatcacct cgatcgtgcg caagatggag cgtaagggtc
     1201 gcagcttcga agagatctac gacgtgctcg gcgtgcgcgt gatcgtcgac gagaagaagg
     1261 actgctacgg cgcgctcggc gtaatccaca ccatgtggca ccccatcccc ggcgaattcg
     1321 acgactacat cgccacgccc aaagagggcg tctaccagtc gatccacacc gccgtgatgg
     1381 ggccggatgg ccatccggtc gagattcaga ttcgcaccca cgagatgcac catatcgccg
     1441 agttcggcat cgcggcgcac tggcgctaca aagagggtgc ccggccggat cccaacgttg
     1501 aggccaagat ctcctggctg cgccagctga tggactggcg cgacgaggtg gtcgacgcgc
     1561 acgagttcgt cgagtcgctc aaatccgatg tcttccagga gatgatctac gtcttcactc
     1621 ccaggggcga catcatcgaa cttccggccg gggctacccc ggtcgatttc gcctaccgca
     1681 tccacaccga ggttggccac caatgcgtcg gcgccaaagt cggtggtcat ctggtgccgc
     1741 tcaacacccg gctacagaac ggccaggtcg tgcagatcat gaccagcaag accaaagtcg
     1801 ggccgagccg cgactggctc ctctcgagca gcggctacat cacgaccgcc tcggcgcgtg
     1861 agaaggtgcg ccagtggttc cggcgccagg agcgcgagga gaacatccag caggggcgcg
     1921 acctgctcga gcgcgagatg cgccggctgg gcgtcgaggc caagctggat gacacgctca
     1981 agcacttccc gcactaccag aagctggacg acttcctggc ggccatcggc tacggcgcgg
     2041 tcacgccgca gcagatcgcg acgcggctgg ccgagcagga ggaccgcacc gtgctggcgc
     2101 agacggcgcc ctcgacgccg gccctgccgc ccagtctgcg cgtcaccggc gtgggcgacc
     2161 tctacacccg gctggcgacc tgctgcaagc cagtctacgg cgaccagatc atcggctatg
     2221 tcacccgtgg ccgcggcatc accgtccatc gcgccgattg ctacaacgtg cgccgcgtgg
     2281 ccgagcccga gcggctggtg caggtgagct ggggcgccga gcagaagcgg ctctacccgg
     2341 tgagcgtccg gctggaggcc tgggaccgcg tcgggctgct gcgcgacatc accacgctgg
     2401 tggcagacgc gaagatgaac atgcagtcgg tgctgaccag cgtcaacgac gaccgcacgg
     2461 tgacggtgct gatgacgctg gaggtcgaga gcgtgcgcca gttgagccag atcctgcaga
     2521 agctcgaagc cgtccgcgac gtctacgacg tgcgccgcga cgccacgccg aacatgtcac
     2581 ggtagccacc gcgcagatcc cgctcgcgaa atcggcacac gaggttgatg ctgtaggggc
     2641 agacctccgt gtctgccctc gtggccctac tgcccagccg caccaacgcc acccacggcc
     2701 cctctatccc cgttgtcggt gcgccggaaa accagggcag acacggag
//
LOCUS       DASUBI010000276         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_793194, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000276 DASUBI010000000
VERSION     DASUBI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793194"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..546)
                     /gene="ndk"
                     /locus_tag="VFU72_05265"
     CDS             complement(88..546)
                     /gene="ndk"
                     /locus_tag="VFU72_05265"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5422929.1"
                     /translation="MAVERTLIIVKPDAVQRGLVAEVLRRLERRGLKFVGLKLMRIPR
                     ELAERHYEEHREKGFYAGLVGFITSGPVVVGVIEGPDAIAVTRATAGATNPAGAAPGS
                     IRADFGLTIGQNIIHASDSAASAAREIALFFQPAELLDYQRAIDPWITES"
     gene            complement(658..987)
                     /locus_tag="VFU72_05270"
     CDS             complement(658..987)
                     /locus_tag="VFU72_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422930.1"
                     /translation="MEAQLLELLDYQPVLAAIASTRDGLLVASVGLADEDAEAVAAAG
                     SAVAAGEAEPVMTIALKGGDLYVALGQELMLLALAETDAAGAGAGLAGVMLEKVGILD
                     SLLAGEG"
     gene            complement(987..>2476)
                     /locus_tag="VFU72_05275"
     CDS             complement(987..>2476)
                     /locus_tag="VFU72_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5422931.1"
                     /translation="DGVAPFAYGAQAGEPAAAEHEPAEAPAEPVSGVSQLFTLATDED
                     ILAARPPEERPSGYTQMLNSLDELGLQPFATAEPPRPSTPFSATAAAPTATPEPAVEA
                     PVAAAPEAVAEPEPEAQPEAAAAGPEAEPAPELDFAAELTAGWEEIDQEIEQAIPSGL
                     TAGYTEELRRIDDSGLEPFNVDAVSGVETPDWLQAFELPATPEPAVESIAELIAEPMT
                     EPLDEASAPAAEAAAEAPPAGDLLFDWPGDDDLAQAVPGDLPRGFTMEFKELEASGIE
                     PFVFGEPPAPAPASANTGIIDEEDLRGITEELEAERLQAAAAAAPAPAPAHAEVVEVA
                     EVVVSERDGEALRTSIERLGLDEALFERARAAKTQLVGQGQISGLKQLPGSEPPGPTL
                     AELQEAVAANPDDGAARQQLASVLVAAGEGRQALEQYRWLYHHQQAGEEAIGALEKLA
                     AERDMVAAGAERLIGAIYRRDGQLTRSAAHYQTALELQLRAKERS"
BASE COUNT          319 a          972 c          805 g          380 t
ORIGIN      
        1 catgctgagc gcactcgaag gatccgtgcc tcacgcgcca cccaacctgc tcgtcaacct
       61 ctccgtttca cgtgaaacgg gcgccgctca ggactccgtg atccaggggt cgatggcgcg
      121 ctggtagtcg agcagctctg ccggctggaa gaagagggcg atctcgcgcg cggccgaggc
      181 ggcgctgtcc gaggcgtgga tgatgttctg gccgatggtc agcccgaagt cggcgcggat
      241 cgagccgggg gccgcgccgg ccgggttggt ggcgccggcg gtggcgcgcg tcaccgcgat
      301 cgcgtccggc ccctcgatca cgccgaccac caccggcccc gaggtgatga accccaccag
      361 gccggcgtag aaccccttct cccggtgctc ttcgtagtgc cgctcggcca gctcgcgggg
      421 gattcgcatc agcttcagcc ccacgaactt caagccgcgc cgctccagcc gccgcaagac
      481 ctccgcgact aacccgcgct ggaccgcatc cggcttgacg atgatcagcg tgcgctcgac
      541 cgccacgtgc cctccctgtt tctgtttctg ttcctgttcc cgttcctgtc agcaaacgac
      601 cgccggccac aggaccggcg gtcgcaccac gtctcctcga atcgtcgcgg tgccgggcta
      661 accctcgccc gccagcaggc tatcaaggat accgactttt tcaagcatca cgccggccag
      721 ccccgcgccc gcgcccgccg cgtccgtctc cgccagggcc agcagcatca gctcctgccc
      781 cagcgcgacg tagagatcgc cgcccttgag cgcgatcgtc atgacgggct cggcctcgcc
      841 ggccgccacc gccgagccgg ccgcggccac ggcctcggcg tcctcgtcgg ccaaccccac
      901 gctggccacc agcagcccgt cgcgcgtgct ggcgatcgcc gccagcaccg gctggtaatc
      961 gagcagctct agcagttgcg cctccattac gaccgctcct tcgcacgcag ttgcagctcc
     1021 agcgccgtct ggtagtgagc cgccgatcgg gtcaactgcc catcccggcg atagatcgcc
     1081 ccgatcagcc gctcggcgcc ggccgccacc atgtcgcgct cggccgccag cttctcaagc
     1141 gccccgattg cctcctcgcc cgcctgctgg tggtggtaga gccagcgata ctgctccagc
     1201 gcctggcgtc cttcgcccgc cgccaccagc accgaggcca actgctgccg cgccgcgccg
     1261 tcgtcgggat tggcggcgac cgcctcctgc agctcggcca gcgtcgggcc gggcggctcg
     1321 ctccccggca actgcttcag gccgctgatc tgcccctggc caaccagttg cgtcttggcc
     1381 gcccgcgcgc gctcgaagag cgcctcatcc agtccgagcc gctcgatcga agtccgcagc
     1441 gcctcgccgt cgcgctcgct caccaccacc tcggcgacct ccacgacctc ggcgtgtgcc
     1501 ggagcggggg cgggcgccgc cgcggccgcg gcctggagcc tctcggcctc caactcctcg
     1561 gtgatgccgc gcagatcctc ctcgtcgatg atgcccgtgt tcgcggaggc cggcgccggc
     1621 gcgggcggct cgccaaagac gaatggctcg atgccggagg cctccaactc cttgaactcc
     1681 atggtgaagc cgcgcggcag atcgcccggc accgcctgcg ccagatcatc gtcgcccggc
     1741 cagtcaaaga ggagatcgcc ggccggcggc gcttccgcgg ctgcttcggc cgccggcgcg
     1801 ctcgcctcgt ccagcggctc agtcatcggt tccgctatca gctcggcgat tgactcgacc
     1861 gccggctcgg gcgttgccgg cagctcgaag gcctgcagcc agtcgggcgt ctcgacgccg
     1921 gagaccgcat ccacgttgaa cggctccagg ccgctgtcgt cgatgcgccg caactcctcg
     1981 gtatagcccg cggtcaagcc ggaggggatg gcctgctcga tctcctgatc gatctcctcc
     2041 cagccggccg tcaactcggc cgcgaaatcg agctcggggg caggctcggc ctcaggcccg
     2101 gctgccgccg cttccggctg ggcctccggc tccggctctg ccaccgcttc cggcgcggcc
     2161 gcgaccggcg cttcgactgc cggctctgga gtggccgtcg gcgcggctgc cgtcgcgctg
     2221 aatggggtgc tcggccgggg cggctcggcc gtggcgaacg gctgcaagcc cagctcatcg
     2281 agcgaattca gcatctgcgt gtagccggac ggccgctcct cgggcgggcg ggccgccagg
     2341 atgtcctcat cggtggccag ggtgaagagt tggctgaccc cgctgacggg ctccgccggc
     2401 gcctcagccg gctcgtgctc cgcggccgcc ggctcgcccg cctgggcacc gtaggcgaag
     2461 ggcgccacgc catcgc
//
LOCUS       DASUBI010000277         8822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_631807, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000277 DASUBI010000000
VERSION     DASUBI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631807"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1148..1981)
                     /locus_tag="VFU72_05280"
     CDS             complement(1148..1981)
                     /locus_tag="VFU72_05280"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5422932.1"
                     /translation="MALLCRVLQIARAGYDAWARRGVAARARADVALTGPIARIQEES
                     RGTDGAPRVHAALRARGGHCGARRVAWLLRAAGLVGCHRRRRARTTVADPAGPPAATL
                     IARDFSAPAPNRWWIGDITSVSTWAGWLSLAVLLDVHARRVVGWARADHRRAALALEA
                     LALALADRRPAAGLVQHPDRGCQDTAGVYQEALAARGIAVSMPRAGEGDDNALAERCF
                     ATLKGELVDTRPWPTRRAARQAIFAWIAVFYHRRRVHSALGDQRPVAFARARAAAAPA
                     A"
     gene            complement(2026..2382)
                     /locus_tag="VFU72_05285"
     CDS             complement(2026..2382)
                     /locus_tag="VFU72_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5422933.1"
                     /translation="MGLRRFCGHPDTGDDGARKGVHGGQDACALPPAFRAEAVELART
                     SGRGIPQLARELGIAEQALRGWVTRAYSDAGRGQPGALTTAEREEWRRLRREVTVRQQ
                     EREILTKAAASFAQET"
     gene            complement(2788..3615)
                     /locus_tag="VFU72_05290"
     CDS             complement(2788..3615)
                     /locus_tag="VFU72_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5422934.1"
                     /translation="MVGWIGAERAIHRAPTTYRWSLADYPDLHPEGVTIQSRTGITIA
                     GRFFPGRSRTTIILSHGYGDNQERMLPWANFLHQAGYSVVTYDMRNRGASGGDAITVG
                     ALEQQDLLLVIDAVAARPDVDRDKIGALGVSLGGATTILTAAQDQRIKAVVDDCGFTD
                     VTGVLGTAFEYFLHLPPFPFAPVAVKIAELRTGQQVAKVRPIDVVGRISPRPIFIILG
                     LADPAVPPVNSARMYAAEGEPKQVWFVPGAKHNGSREVAGAEYEQRIVAFFCQSLGE"
     gene            complement(3857..6478)
                     /locus_tag="VFU72_05295"
     CDS             complement(3857..6478)
                     /locus_tag="VFU72_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEU5422935.1"
                     /translation="MDTQQERQATRFDEAVRIEHQIEEIDRRVQALERWAATMYWNPL
                     LPATPRRQPVQSTPPQAPAGSPDVVLTTAAVAPAAPAPRSTPPAGPAPAPPPPTRTLP
                     PVARPPTLAPRWTLGDLDQLLSGRGLAWLGGLAILLGALFFLGLAFTRGWIGPEARVG
                     IGLVAGIGLVTGGAWRFTKHEVLFGHVLVATGLGVLSLAFVAATRLYDLVPVEAGLAG
                     ALLVAIAAALIAIRADSQLVAGYGLVTALAAPPLLGAAPTFATIAFLATALIGTTAIA
                     LYRTWAWLPPVAFVLAAPQLAEWIFGGLPASFAPALAALAGFWLLNSLAAGGEEFRVR
                     HNRLRPTSATLLLANAAFLVWAGFGHLARHDAEGARGLLLLIVAVAHGLLGGYFLYED
                     GERHPFGLLACGTGLATFTIAIPVQLGGPVVPIAWAAEAAALAWVYAWRRHGYSGAAA
                     LVLGGLAVAHLASIEYPFWQIASEQQNTAPFFNAQGGTLGFILLALAVAGFFVRSWPV
                     RAGLTSAGAILVLYALPFELSGLALLACFAAALAIEVALNRAFLLLRARRVSGAAPVM
                     FRVALYLPSALAAVLTAGHALLFELPPRLLGVLPATPFSDNATLAAGMLVAASLIAGE
                     LAGQPATRRAGRLAAIAVFAYLLPFELHSAAVVAGWAALTLALGGLAAWDHQGERYYL
                     GAAAMLVALGFVVGLTLVAPPERLVVDGRTTLRHPPLWCGATAAFGALAAILALGAWR
                     YQKRRWARSLAILAGGALIYLLSVGVVDEFQGRVGGAVAFASLRKGGQVALSILWAAL
                     GGAAFVGGVIRRITSLRLAGLALLALATGKVVLVDLASLDATYRVPSFIALGLFLLAS
                     SYVYQRLRPQPPRQEAH"
     gene            complement(6842..8152)
                     /locus_tag="VFU72_05300"
     CDS             complement(6842..8152)
                     /locus_tag="VFU72_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5422936.1"
                     /translation="MSVMLPEQITPQHHERRAVIYVRQSTPKQVREHRASHANQYALV
                     DRAVALGWVRPRVQVIDDDLGQSGRDGERPGFQALVSAVSLGEVGLILAFEASRLARN
                     NADWYRLLDLAALVGTLIADVDGVFDPRRYHDRLLLGLQGMRSEAEWPCQRQRLDAGR
                     QRRIAEGPYRQLLPTGLVRQEDGRVVLDPDQQVRHAVELVFARFAALGSCQKVLRSLR
                     DAGVLLPRRQTGGLHAGQLLWKRPNESALDELLTNPADAGAFVFGRTGRAPGTRPGRR
                     APAPRRPLAWALAALAVAGWGYVHYLLIVRTWSPVLEHRLAYEAARNGALPGPSPGMS
                     LLSNVLVALELLVAPPLIGLLAAARAGRCQPLVGAAAGLLTILSALLVPAILFAVVFA
                     RAGIATGNEPTDWELTLVFGVLGLVLGAAGGLLALRGAKSPAGR"
     gene            complement(8660..8822)
                     /locus_tag="VFU72_05305"
                     /pseudo
     CDS             complement(8660..8822)
                     /locus_tag="VFU72_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1408 a         3061 c         3257 g         1096 t
ORIGIN      
        1 tcatgccggc gctgcagcgt gaggcggcgg acatgatgga caacctgctg ctagccggca
       61 ggtagtcgtg gatgggtgaa cccggcggag ggcgccgatg cacgcaatag gcgtcgggtt
      121 cccgtgtccg gccacggcac ccgcccacaa tggcatggtg attacctcgc tgtcactaca
      181 ggccagggcc gggccgctcg ccagcttgta cgttggcggc agccggcgcc acaacgcgtc
      241 caccacgtcc agccacaagc agaaggccgc gaagtgcgct atcatcatcc ggtcctcctc
      301 gccagggcgt ttggcgtagt gtgcggtaac cccatctgcg ctaggctgag gctcttcact
      361 ccgctcagta gcccgccgcg ttcggtatga tcgcccaaag cgaatggcgt ctgcgaacag
      421 tggggggagg ggcatgctct tggaggttgc cttggctgaa aatgtgtaag caccgcgctc
      481 tgcagcacga tggtaggtat ggtgctggcc gcccaggacg ggcaccgcac gtctcggacc
      541 ggcgcgcccc ccggtctcct cgcctggcgg cgcaggcagg gcttggccgc gtccctggtg
      601 gggcggttgc agtaggcgac gtactcgcgc cgcgcgtgcg ccaggtggcg ctccccaagg
      661 acgaggcggt catgcaggca gcgcgcacac ggcgttcgcc cgcagcgacg gacgcgaacg
      721 tcgccgcgga tttgccgtcg ttgtcgcaga tcaagtaccg tgggcgctgg ccgaaggtgg
      781 aagcctcgcg gagctgctgc gcggcccagg cggcggtcgg gtggcgcagg aaggtcaccc
      841 agcgttggct acgcagaagg ggggcatggc agccccgcag gtaggtctgg atcgtgcgct
      901 gcgccacccc gatgcctagc ttggcgagcg cgccgcgccc cagcgcggta ctccgccgcc
      961 aagcggcgga tgagggcgac agtcgctgcc gggagcggcg gacggcccgg cccggggcgc
     1021 gacttccgcc gccagcgggc gcgaaagccc gcgcgatgcc agcgcagcag tgtcccgggc
     1081 tgcacgagga gcaaggcctg ccgccaggcg cggacgggct taggtcagtt tcgtggacag
     1141 gatgaggcta ggccgctggc gccgccgccg cacgcgcccg cgcgaaggcc accgggcgct
     1201 ggtcaccgag ggccgagtgc acccgccggc gatggtagaa gaccgcgatc catgcgaaga
     1261 tggcctgccg cgccgcccgc cgggtgggcc aggggcgcgt gtcgacaagt tcgcccttca
     1321 aggttgcgaa gcagcgctcc gccagggcgt tgtcgtcgcc ctccccggcc cggggcatcg
     1381 acacggcgat gccccgggcg gcgagcgcct cctggtacac gccggcggtg tcctggcacc
     1441 cccggtcggg gtgctggacg aggcccgccg ccggccgtcg gtctgccaac gccagcgcca
     1501 gggcctcgag ggccagcgcg gcgcgccggt ggtcggccct ggcccagccg accacccggc
     1561 gcgcgtggac atccaggagc accgccagag agagccagcc cgcccaggtt gaaacggacg
     1621 tgatgtcgcc gatccaccag cgattcggcg ccggcgcgct gaagtcgcgc gcgatgagcg
     1681 tggccgccgg cggccccgcc gggtcggcga cggtggtgcg ggcacggcgc cggcggtggc
     1741 acccgacgag gccggccgcc cgcagcagcc acgccacccg gcgggcgccg cagtgcccgc
     1801 cccgcgcccg cagcgcggcg tggacccgcg gcgcgccgtc ggtcccgcgg ctctcctcct
     1861 ggatccgggc gatcggcccg gtcaacgcca catccgcccg ggcgcgggcc gcgaccccgc
     1921 ggcgcgccca ggcgtcgtag ccggcccggg caatctgcag cacccggcac agcagggcga
     1981 tcggctaggc ggcccgctcc gcgtggatga accgataccg gctcatcagg tctcctgcgc
     2041 gaaggaggcc gccgcttttg tcaggatctc gcgctcctgc tggcgcaccg tgacctcgcg
     2101 gcgcagccgg cgccactcct cgcgctccgc cgtggtcagc gcgcccggct ggccgcgccc
     2161 ggcgtcgctg taggcgcgcg tgacccagcc gcgcagcgcc tgctccgcga tccccagctc
     2221 ccgggcgagc tgggggatgc ccctgccgct ggtccgagcc aactcgactg cctcggcgcg
     2281 gaaggccggg ggtagggcgc atgcgtcttg gccaccgtgg actcctttcc gagcaccgtc
     2341 gtccccagta tccgggtgtc cacaaaaccg acgcaagccc acggacgtgt ccggcgagcg
     2401 gcacgaggag cgtccgatcc gcccgggtca tgggcaggtg cgcggcgctc cgccgtagca
     2461 tcagcaggtg ctggcgcagc agatcgcccc ggtccccacc gcgtgcgtcg caagtgccgg
     2521 cgttgcccag atgccggcgt ggagggtgcg ccgcatgccc gcgcctattc tggtcaaccg
     2581 acgacggcac gcctgcctaa acagcaccac ggccattgcc ctggccccac gcttccgccc
     2641 cacacgagac gccattacgc ctcgccgaag cgatgctcgt cgcctgatgg agtgatcaat
     2701 ggcgtagact tgcggcgaag gcccggcccc gatggatgtt gagccaggac agccccacac
     2761 gggccgcaac gaatcaccag acacgcgcta ctcgccgagg gactggcaga agaacgcgac
     2821 gatccgctgc tcgtactcag cgccggccac ctcgcggctg ccgttgtgct tcgcccccgg
     2881 cacgaaccag acctgcttcg gctcgccttc cgccgcgtac atccgcgcgc tgttgaccgg
     2941 tgggaccgcc gggtcggcca ggccgaggat gatgaagatt ggtcgtgggc tgatccggcc
     3001 aacgacgtca atcgggcgca cctttgctac ctgctggccg gtccgcagtt cggcgatctt
     3061 caccgcgacc ggcgcgaagg ggaagggcgg cagatgcagg aagtactcga aggcggtgcc
     3121 aagaaccccg gtcacgtcgg tgaagccgca gtcatccacc acggccttga tgcgctgatc
     3181 ctgcgctgcg gtaagaatcg tcgtcgcgcc gccgagcgag acacccagcg cgccgatctt
     3241 gtcccggtcc acgtcgggcc gtgccgccac cgcgtcaatg accaacagca gatcctgctg
     3301 ctccagcgcc ccgacggtga tcgcgtcgcc gccgctcgcg ccgcggttgc gcatgtcata
     3361 ggtgacgacg ctgtacccgg cctggtggag aaagttggcc caggggagca tccgctcctg
     3421 gttgtcgccg tagccgtgcg acaggatgat cgtcgtccgg ctgcggccgg ggaagaaacg
     3481 cccggcgatg gtgatgccgg tgcggctctg gatggtcacg ccctccggat ggaggtccgg
     3541 ataatcggcg agcgaccagc gataggtggt cggtgcccgg tggattgccc gctccgcgcc
     3601 gatccagccg accatgccca gtccggcagt cgcgacgacg agcagcagcg tgaccaggag
     3661 cagaacgcta cgccggagta gcctggcgtg tcgtcacggg cgatggccgg acgccattct
     3721 ggtggcggct tgtctcgctg gctgaccgga agatgcaagt ctggtcagga gatcccttcc
     3781 ccgtgtcgcc gggggccgac cggcccccgg cgacgccacg cctataccat gctggcgctc
     3841 tcgccatccc gggccatcag tgcgcctcct gccgtggagg ctgggggcgc aaccgctggt
     3901 agacgtagga acttgccagc aggaagaggc cgagggcgat gaacgagggc acccggtagg
     3961 tcgcatcgag cgacgcgaga tcgaccagaa cgaccttgcc ggtcgccagc gcaaggagcg
     4021 ccaggccggc caggcgcagc gacgtgatcc ggcgaattac cccgccgacg aaggcggccc
     4081 cgccgagcgc cgcccagagg atcgagagcg cgacctgccc gcccttccgg agcgacgcga
     4141 aggcgaccgc gccaccaacg cgcccctgaa actcgtcaac caccccgacc gagagcaggt
     4201 agatgagtgc gccgcccgcc aggatggcga gggaccgcgc ccagcggcgc ttctggtacc
     4261 gccaggcacc gagggccagg atcgcggcga gggcaccgaa ggccgccgta gcgccacacc
     4321 agagcggcgg gtggcgaagc gtcgtgcgcc catccacaac gagccgctcg ggcggggcga
     4381 ccagcgtcag gccaaccacg aagccaagcg cgaccagcat cgccgccgcg ccaagatagt
     4441 accgctcgcc ctggtggtcc cacgctgcga ggccgccaag cgcgagtgtc agcgccgccc
     4501 agccggcgac gaccgccgcg ctgtgcagct cgaacgggag caggtaggcg aacacagcga
     4561 tggccgcgag acgcccggcc cggcgagttg ccggctgccc agccagttcg ccggcgatca
     4621 ggctggcggc aaccagcata cctgcggcca gcgtcgcatt gtcgctgaag ggcgtcgccg
     4681 ggagcacgcc caggaggcga ggcggcaact cgaagaggag cgcgtggccg gcggtgagga
     4741 ccgcagccag cgcggatggg agatagagtg cgacgcggaa catcaccggc gcggcacccg
     4801 atacgcggcg agcccgcagg agcaggaagg cgcggttgag ggcaacctcg atcgcgagcg
     4861 ctgccgcgaa gcaggcgagt agcgcgagac ctgagagttc gaatggcaag gcatagagga
     4921 caaggatcgc cccggcactg gtgagaccag cgcgcaccgg ccacgagcgc acgaagaagc
     4981 cggcaaccgc gagcgccagc aggatgaagc cgagcgtacc gccctgtgcg ttgaagaacg
     5041 gtgcggtatt ctgctgttcg ctagcgatct gccagaacgg atattcgatg ctcgccagat
     5101 gggcgacagc aaggccgccc aggacaagcg ccgcagcgcc gctgtagccg tgccgccgcc
     5161 aggcatagac ccaggcgagc gccgcagcct cagcggccca ggcgatcggc accaccggcc
     5221 cgccgagctg gaccggaatc gcgattgtaa acgtcgcgag tcctgtgccg caggcgagga
     5281 ggccaaaggg atggcgctcg ccatcctcat agaggaagta gccgccgagg aggccgtggg
     5341 cgaccgcgac gatcagcaga agaagcccgc gcgcgccctc cgcgtcgtgc cgcgcgagat
     5401 gcccaaagcc ggcccagacc aggaaagcgg cgttcgccag cagcagcgtg gccgaggtcg
     5461 ggcgcagccg gttgtggcgc acccggaact cctcgccgcc cgccgccagg gagttgagca
     5521 accagaagcc ggcgagggcc gcaagtgctg gtgcgaagga agcgggcagc cctccgaaga
     5581 tccattcggc gagctggggt gcggccaaga cgaaggctac cggtggcagc caggcccagg
     5641 tgcggtagag agctattgcc gttgtgccga tgagggccgt cgccaggaag gcgatcgtcg
     5701 cgaaggtcgg ggctgcgccc agcagcggcg gcgcggccaa cgccgttacc aggccgtagc
     5761 cggccacgag ttgcgaatcg gcccggatgg cgatgagcgc cgcggcgatg gccacgagca
     5821 gcgcgccggc gaggccagcc tcgaccggga cgaggtcgta gagtcgggtg gcggcgacga
     5881 aggcgagact gaggaccccg aggccggtgg cgaccaggac atgcccgaag agaacctcgt
     5941 gcttcgtgaa gcgccacgca ccgccagtga cgaggccgat ccccgcaaca agcccgatac
     6001 cgacccgcgc ctcgggaccg atccagccgc gcgtgaacgc gaggccgagg aagaagagcg
     6061 cgccgagcag gatcgccagc ccgcccagcc aggcgaggcc gcgcccactc agcagctggt
     6121 cgaggtcgcc gagcgtccat ctcggcgcca gcgtcggtgg acgagctacc ggcggcagcg
     6181 tacgggtcgg tgggggcggg gccggcgccg gccccgcggg tggcgtagat cggggagccg
     6241 gcgcggcggg cgccactgct gccgtcgtca gtaccacatc gggggagcca gcgggagcct
     6301 gtggtggcgt gctctgcact ggctgacggc ggggtgtcgc aggcagcaat gggttccagt
     6361 acatcgtcgc agcccagcgc tccagcgcct gcacacgcct gtcgatctcc tcgatctggt
     6421 gctcgatcct tactgcttca tcgaaacggg tggcctgtcg ctcctgttgc gtatccattg
     6481 ttctatcccc ctctgagcgc cacaggagct agccggcgca gaccgggccg cgcagccggg
     6541 cgcagatcgc gcgacactat caccacgctg atcgtagtcg ggaactgtca ccaggcatat
     6601 tgcagccggt gaacggcacg taaatcagcg gcaggggagt cgcctcgttc gaccgggctg
     6661 cttacgctac ggtgcctgtt cccccgccat tcatgatctg caccctcgct gcctacctcg
     6721 cgaggtgagc ataggagcat acgttcgccc agacgtgcgc cggtacgccg atcgcagcgc
     6781 cgggcgagtt gccgaacata cgctgcgcgt caacacccga acccttgccg caatcgcagc
     6841 atcagcgccc ggccggcgac ttcgcgccgc gcaaggcgag cagaccgccg gcagccccga
     6901 gcaccaggcc aagcacaccg aagacaaggg tcagctccca gtcggtcggt tcattaccag
     6961 ttgcgatgcc cgcccgcgcg aacacgaccg cgaagaggat ggcgggcacg aggagggcgc
     7021 tgaggatcgt cagcagcccg gctgctgcgc cgacgagtgg ctggcatcgt ccggcgcggg
     7081 cggcggcgag caagccgatc aggggcggcg ccactaggag ttctagcgcc accaggacat
     7141 tgctcagtag gctcatcccc ggtgaaggac cggggagggc gccgtttcgc gccgcctcat
     7201 acgcgaggcg atgctcgagg actggtgacc aggtgcggac gatgagcagg taatgcacgt
     7261 agccccagcc ggcaacggcg agcgcggcaa gcgcccaggc gagcgggcgg cgcggcgcgg
     7321 gggcgcggcg gccggggcgc gtgccgggcg cgcggccggt ccggccgaag acgaaggccc
     7381 cggcgtcggc cgggttggtc agcagctcgt cgagcgccga ctcgttcggc cgcttccaga
     7441 gcagctgacc ggcgtgcagc ccgccagtct gccgtcgcgg cagcagcacc cccgcgtcgc
     7501 gcaggctgcg cagcaccttc tggcagctgc ccaacgcggc gaagcgcgcg aagacgagct
     7561 cgacggcgtg gcgcacctgc tggtcggggt ccagcacgac gcgcccgtcc tcttgccgca
     7621 ccagcccggt cggcagcagc tggcggtagg ggccctcggc aatccggcgc tggcgccccg
     7681 cgtccagccg ctggcgctgg cagggccact cggcctcgct gcgcatcccc tgcagcccga
     7741 gcaggagtcg gtcgtggtag cgccgcgggt cgaagacccc gtcaacgtcg gcgatgaggg
     7801 tgccgaccag ggcggcgagg tcgagcaggc ggtaccagtc ggcgttgttg cgcgccagac
     7861 ggctggcctc gaaggcgagg atgaggccga cctcgcccag cgagacggcg ctgaccagcg
     7921 cctggaaccc cggccgctcg ccgtcccggc ccgactggcc gaggtcgtcg tcgatgacct
     7981 gcacgcgcgg gcgcacccag ccgagcgcca ccgcgcgatc gaccagcgca tactggttgg
     8041 cgtggctggc gcggtgctcg cgcacttgct tgggcgtcga ctggcggacg tagatgaccg
     8101 cccgccgctc atggtgctgc ggggtgatct gctcggggag catcaccgac atgcgctacc
     8161 tcctgcagga gcgcgacgag cgcctgacgg ccctgcgcct gctgcgccgg ggcgagggtg
     8221 ggccacaccg ccgccggcgg gcggggcggc gtgagcggga gcggcggcga cgtggccagc
     8281 gggagcggga gcggcagggc gagctggcgt ggcgggcgcg ggcggggcag cgccatgggc
     8341 atcctcctgg ccgggtggcg ggttgggcac ctcgtccagg caggcgagca cggcgaggag
     8401 ggcggcgacg ccgggggccg agaggcggca gggcgagggg gggcggggcg gcccgccgag
     8461 gctggtggcg gcgacgggga tgaggcgctc gacgccgggg gcgagccaga cgacgcagac
     8521 gcgccccagg tgctgctgct tggtcacccc gaggcagggg aggcgcaggc cgtagagcgg
     8581 gtgcgttggg tcggtgacct cgatctgctc agacggcggg acgctcaggc caacaggggt
     8641 actgtcttga gtggatgtgg agccacgtcg ccagagtggc ggccgtgcga tcggggagga
     8701 catcgaccgg acagtggcgt tccagatcga cgaggatcgt gccaaagacc tggccccgtc
     8761 ggtagctcca atcgtctacc cccagcacgc gcggcgtcgg cgcgggcggg aggtcggccc
     8821 gc
//
LOCUS       DASUBI010000278         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_261557, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000278 DASUBI010000000
VERSION     DASUBI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261557"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1378
                     /locus_tag="VFU72_05310"
     CDS             170..1378
                     /locus_tag="VFU72_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5422937.1"
                     /translation="MSTSPDAAMGRLENHYRKASPQEFKRRHELLYKLMAQKGLDGVI
                     VYGGYKEMYQANARWLTGMREAMQFYAFFPARGEPTAWNALFPHLIAAQRMSAIPDTR
                     WGGPSIAEAVAGRIKELGLRHGKIGLVGVHSARGITLPMDHWLIWQDELPHVEFVNVT
                     RDIEDMQLVKSDEELQFYAKGAEYTDYTMAELIKAIEPGVDEVTLFGRMIIAAHEIGG
                     QLDFALLGSTPMANPDMPYPWHVPTERVLAQGDIVLNEISTNYAGCSGQLIVPISLGE
                     PTPEYRELYDIAAESLERARLVLRPGATQDDILEAARPITDAGLTSQASFIHGWPNPP
                     MRPAVRLGERGAAEGRDQFVLQENMMIMIEPNPTTPDKKRGIFLGALHVVTPEGGRNL
                     HKYPLAFVRR"
     gene            1436..>2089
                     /locus_tag="VFU72_05315"
     CDS             1436..>2089
                     /locus_tag="VFU72_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5422938.1"
                     /translation="MAEHASRLVDRLTAGHLSRRTLLRSGALAGAGASVLGLLAACGG
                     SSSTTATASSGGGAATTPAATQASGGGSSSTAAATQASGSPVSGTQAAGGGKPKFDKV
                     IIAESADVRFFDPTIRPTSTDATVLVNIYDNLTQLNEKFELVPGLATKWELGANDQQT
                     WTFTLRQGVKFHNGEPFNADTITAWYDRLVNIPTLVKDQPSSIQQIASVDKVSKVDDY
                     "
BASE COUNT          366 a          738 c          703 g          282 t
ORIGIN      
        1 gcatcttcta cggcaatctc tgcgtggtga ccaaggacgg ctgccgcaac ctgcacaagt
       61 tccccgacga gttcgtggtc gtgcagtagg cagcgagacg gaggcgcggg gcaacgcgcg
      121 gcgcgttgcc ccgcgcgacc cccgcgacga gtgcgggagg aggtagcgga tgtccacttc
      181 cccggacgcg gccatgggac ggctcgagaa tcactaccgc aaggcgtcac cgcaggagtt
      241 caagcgccgg cacgagctcc tctacaagct gatggcccag aaggggctcg acggcgtcat
      301 cgtctacggc ggctacaagg agatgtacca ggccaacgcg cgctggctga ccggcatgcg
      361 tgaggcgatg cagttctacg ccttcttccc ggcgcggggc gagccgaccg cctggaacgc
      421 gctcttcccg catctgatcg ccgcccagcg catgtccgcc atccccgaca cccgctgggg
      481 cgggccgagc atcgccgagg cggtggccgg gcgcatcaag gagctggggc tgcggcacgg
      541 caagatcggg ctggtcggcg tgcactccgc gcgcggcatc accttgccga tggaccactg
      601 gctgatctgg caggacgagc tgccccacgt cgagttcgtc aacgtcacgc gcgacatcga
      661 ggacatgcag ttggtcaaga gcgacgagga gctgcagttc tacgcgaagg gcgccgagta
      721 caccgactac accatggccg agctgatcaa ggcgatcgag ccgggggtcg acgaggtgac
      781 cctcttcggg cgcatgatca tcgcggccca cgagatcggc gggcagctcg acttcgccct
      841 gctcggcagc acgccgatgg cgaacccgga catgccctac ccctggcacg tgcccaccga
      901 gcgggtgctg gcccagggcg acatcgtgct gaacgagatc agcaccaact acgccggctg
      961 ctccggccag ctgatcgtcc cgatcagcct gggcgagccg acgccggagt atcgcgagct
     1021 ctacgacatc gcggcggagt cgcttgagcg cgcccgcctg gtgctgcggc cgggcgcgac
     1081 ccaggacgac atcctggagg cggcgcggcc gatcaccgac gccggtctca cctcgcaggc
     1141 ctcgttcatc cacggctggc cgaacccgcc catgcgcccg gccgtccgcc tgggcgagcg
     1201 cggggcagcc gaggggcgcg atcagttcgt gctgcaagag aacatgatga tcatgatcga
     1261 gccaaacccg accacgcccg acaagaagcg cggtatcttc ctgggcgcgc tgcacgtggt
     1321 gaccccggaa ggcggccgca acctgcacaa gtatcccctc gcattcgtgc gccgctgagg
     1381 cggcgcggtc gtggagatcc gggccgtttc ccggcccgag aggaggagag cgcacatggc
     1441 agagcacgca tcgcggttgg tggatcgctt gacggccggg catctcagtc gccgcaccct
     1501 gctgcgcagc ggcgcgctgg cgggcgccgg cgcctcggtg ctggggctgc tggccgcctg
     1561 cggcggcagc tcgtcgacca ccgccactgc cagtagcggc ggcggcgccg ccaccacgcc
     1621 cgccgccacc caggccagcg gcggcggctc gagctcgacc gcagcggcta cccaggccag
     1681 cggctcgccg gtcagcggca cccaggccgc cggcggcggc aagccgaagt tcgacaaggt
     1741 catcatcgcc gagagcgcgg atgtccgctt cttcgacccg acgatccggc cgacctcgac
     1801 cgacgccacc gtcctggtca acatctacga caacctgacc cagctcaacg agaagttcga
     1861 gctcgtgccg gggctggcga ccaagtggga gctgggcgcc aacgaccagc agacctggac
     1921 cttcaccctg cgccagggcg tcaagttcca taacggcgag ccgttcaacg ccgacacgat
     1981 cacggcctgg tacgaccggc tggtcaacat ccccacgctg gtcaaggatc agccgagctc
     2041 aatccagcag atcgccagcg tcgacaaagt ctccaaggtc gacgactac
//
LOCUS       DASUBI010000279         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_869137, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000279 DASUBI010000000
VERSION     DASUBI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869137"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2201)
                     /locus_tag="VFU72_05320"
     CDS             complement(<1..>2201)
                     /locus_tag="VFU72_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEU5422939.1"
                     /translation="YTVDAACASSLIAIELALRDLQSGACDLALAGGSQVWIPVPALN
                     VFCQLHALSHRQQIRPFDKDADGTLLGEGIGMIVLKRLADAERDGDRIYSVIRGVGVA
                     SDGRGTSVMAPRIEGEELALRRAYAAAGVAPETIELIEAHGTATPVGDVVEVQALARV
                     FGPREGEQARVALGTVKSMISHTIPAAGIAGMIKTSLALYHKVLPPTINVTEPNPKLE
                     LEKTPFYLNTETRPWIHGTPEPRRAGVNAFGFGGINAHVVLEEYRGTPVEDDAPPDGS
                     AARGHAPEWASEVVVLEAGSAAELRARAELLHVALEPLQTGAAPRLTDLAYTLNSALG
                     AVESPLRLAVIATSLADLRKKLERALKRLADPNCRQIKDVSGIYYSAGPLARQGKLAL
                     LFPGEGSQYPNMLADLCLHFPEVRESFDRTDRIYRGHPRGYVLSDVAFPRPSFNAAER
                     AQAEQRLMQMDNAIEAVLTANGAMLLLLRRLGLKPDAYLGHSTGEYSAMIAAGVLDLE
                     SEPEIARFSLALNRGYEAERAREGVPTALMLAIGAPREQVEALAREAGGDLLPAMDNC
                     PHQTVLVGERAAGERAIAILQREGLIYEQLTFDRAYHTRLFAPYSDTLRAAVAEAPVR
                     PAREPIYSCTTCAPYPEQPEAIRELMIEHWHQPVEFRRTIEHLHDEGYRLFVEAGARG
                     NLTAFVEDILRGRDFQAASADTQRRDGITQLNHLVGMLLVHGVALDLSYLY"
BASE COUNT          294 a          771 c          816 g          320 t
ORIGIN      
        1 ggtagaggta gctgagatcg agcgccacgc cgtggaccag cagcatgccg accaggtgat
       61 tcagctgggt gatgccgtcg cgccgctggg tgtcggcgga ggcggcctgg aagtcgcgtc
      121 cgcgcaggat gtcctcgacg aaggccgtca ggttgccgcg cgcgcccgcc tcgacgaaaa
      181 ggcggtagcc ttcgtcgtgc aggtgctcga tggtgcggcg gaactcgacc ggctggtgcc
      241 agtgctcgat catcagctcg cggatcgcct cgggctgctc cgggtagggc gcgcaggtgg
      301 tgcaggagta gatcggctcg cgcgccgggc gcaccggcgc ctcggccacc gccgcccgca
      361 gcgtgtcgga gtagggcgcg aagagccggg tgtggtaggc gcggtcgaag gtgagctgct
      421 cgtagatcag accctcgcgc tgcaggatcg cgatggcgcg ctcgccggcc gcgcgctcgc
      481 cgaccagcac cgtctgatgc gggcagttgt ccatcgccgg caggaggtcg ccgcccgcct
      541 cgcgggcgag cgcctcgacc tgctcgcgcg gcgcgccgat ggccagcatc agcgcggtcg
      601 gcacgccctc gcgcgcgcgc tcggcctcgt agccgcggtt caacgccagg ctgaagcggg
      661 cgatctccgg ctcgctctcc aggtcgagca cgccggccgc gatcatggcc gagtactcgc
      721 cggtgctgtg gcccaggtag gcgtccggct tgaggccgag acggcgcagg agcagcagca
      781 tggcgccgtt ggccgtcagc accgcctcga tggcgttgtc catctgcatc agacgctgct
      841 cggcctgggc gcgctcagcg gcgttgaaac tcgggcgcgg gaaggccacg tcgctcagca
      901 cgtagccgcg cgggtggccc cggtagatgc ggtcggtgcg atcgaagctc tcgcgcacct
      961 cggggaaatg caggcagaga tcggccagca tgttggggta ctgcgaacct tcgccgggga
     1021 agaggagcgc cagcttgccc tggcgcgcca gcggcccggc gctgtagtaa atgccggaga
     1081 catccttgat ctggcggcaa ttggggtcgg ccaggcgctt gagcgcgcgc tccagcttct
     1141 tgcgcaggtc ggcgagcgag gtcgcgatca ctgccagccg cagcggcgac tcgaccgcgc
     1201 cgagcgcgct attgagcgtg taggcgaggt cggtcaggcg cggcgcggcg cccgtctgca
     1261 gcggctccag cgcgacgtgc agcagctcgg cgcgcgcgcg cagctccgcg gccgagcccg
     1321 cctccagcac caccacctcg ctggcccact cgggcgcgtg gccgcgggcg gcgctgccgt
     1381 ccggcggggc gtcgtcttca accggtgtgc cgcgatactc ctccagcacg acgtgcgcgt
     1441 tgatgccgcc gaagccgaag gcattcaccc cggcccggcg cggctcgggc gtgccgtgga
     1501 tccagggccg cgtctcggtg ttgaggtaga agggggtctt ctccagctcg agcttcgggt
     1561 tcggctcggt cacgttgatc gtcggcggca gcaccttgtg gtagagcgcc agcgacgtct
     1621 tgatcatccc ggcgatgccg gcggccggga tggtgtggct gatcatcgac ttcaccgtgc
     1681 ccagcgcgac ccgcgcctgc tcgccctcgc gcgggccgaa gacgcgcgcg agcgcctgca
     1741 cctcgaccac gtcgcccacc ggcgtcgccg tgccgtgcgc ctcgatcagc tcgatcgtct
     1801 ccggcgcgac gccggccgcc gcgtaggcgc gccgcagcgc cagctcctcg ccctcgatgc
     1861 gcggcgccat cacgctcgtg ccgcgcccgt cgctggccac gcccaccccc cggatgaccg
     1921 agtagatgcg gtcgccatcg cgctcggcat cggccaggcg cttgagcacg atcatgccga
     1981 tgccctcgcc cagcagcgtg ccgtcggcgt ccttgtcgaa ggggcggatc tgctggcggt
     2041 gcgacagcgc gtgcaactgg cagaagacgt tgagcgccgg caccgggatc cagacctgcg
     2101 agccgccggc cagcgccagg tcgcaggcgc cggactggag gtcgcgcagc gccagctcga
     2161 tcgcgatcaa ggacgaggcg caggcggcgt ccacggtgta g
//
LOCUS       DASUBI010000280         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_793155, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000280 DASUBI010000000
VERSION     DASUBI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793155"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFU72_05325"
     CDS             <1..790
                     /locus_tag="VFU72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEU5422940.1"
                     /translation="LVEKLWPLVRLTFPKLGLLSQPNSGRSALALAENSPTGKPFNLN
                     SAGSAVQFFPILVAELLSGPLSWFGFVDLPAGEARPRAFGVRPAAGVLVDRIPVSQSP
                     AEAAGLVVGDDLTVLIPIDHPDSEIHGLLAQAGELVEASPGGLRYRLTSERVQALFDQ
                     GVSGPEMLRVLDEHGATLPQAARAQLDGWWDAYGRVRLYDELTVIELADDRLLPELLA
                     GTSLGEKLIYTFSPRLIAIDASAAGELVAELTRLGHAPRLVEGD"
     gene            790..>3088
                     /locus_tag="VFU72_05330"
     CDS             790..>3088
                     /locus_tag="VFU72_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEU5422941.1"
                     /translation="MAAETPAKDILRGYTNAALQMMAKTRGLNVQGKGKEKLVQLLAE
                     TLFSPSRIKPALSELEPLQREVLDRVILAGGRMPTQVLRAALEQENLVERQPPRSLYG
                     YAQPVSGSAYKKGSRKFEDSLARLCALGLVFTTGASSGRMYDLVTPGLRVWIPPQILR
                     HLPPVTVALVTTEPPPEIVAGNPGPLQRDLYTLLSLAERQTIPLTMRGQIMKRALVKL
                     DPELLLSEMTPQTRSEDELGRIPFLRALAEDLGLLSASVGTLAPNPGIQAYLRRPAGE
                     RLAQLYQTYRDSARFNELYRIPNLRISGKGSSTRAAPPPVIAARQRVLREIAELPAEQ
                     WIPLGHLIDRLRRRDYEFLLERPDEVRYEYYYYGRQDFNPYANNAMGVTFEGLNEGEG
                     WTRVEAGMIRVIVTEALAWLGVVDLGWREGEPVAFRVSEAGAHLLRGETPASLEAAPT
                     SNVVVQPNFQLFAFEPTGEDVLFMLDRIAERVRAEQAIEYELTRDSIYRGQQSGLDIA
                     EIIAFLERVSTVELPRNVRRTLAEWGAQHERVTLRRGVGLLHAVDAATLDALYADPEL
                     GPLLGRRAAPDVALVPAANLHTLVPRLLAAGQLPALSEGDDLAPRPVFTIDPEGRVTF
                     RQRLPSIQVLRVLRPLTDGPSQPGQGDTPRLTRESLRRAARSGQSAEAIIDSLARFHD
                     GPLPEEVVAFVKRWAKDWGKGALVEAVLLQLERPELLDDLLADPLLRPCLRRVPGAPA
                     YALVRPGALEAVRAALEERDMPLQGE"
BASE COUNT          429 a         1086 c         1122 g          451 t
ORIGIN      
        1 gctggtcgag aagctctggc cgctggtgcg gctcaccttc ccgaagctgg gcctgctgag
       61 ccagccgaac tccggccgct cggcgctggc gctggcggag aactcgccga ctggcaaacc
      121 gttcaacttg aattccgccg ggagcgcggt gcagttcttc cccatcctgg tcgcggagct
      181 gctctccggt ccgctgagct ggttcggctt cgtcgatctc cccgccggcg aggcacgccc
      241 gcgcgccttt ggcgtgcgcc cggcggccgg cgtgctggtc gatcgcatcc ccgtcagcca
      301 gtcgccggcc gaggccgcgg ggctggtggt cggcgacgac ctgacggtct tgattccgat
      361 cgaccaccca gactccgaga tccacggcct gttggcgcag gcgggagagc tggtcgaggc
      421 atcgcccggc ggcctgcgct accgcttgac cagcgagcgg gttcaggcgc tcttcgacca
      481 aggcgtcagc gggccggaga tgctgcgcgt gctcgacgag cacggcgcga cgctgccgca
      541 ggcggcccgc gcgcagctgg acggctggtg ggatgcctac ggccgggtgc gcctctacga
      601 cgagctgacg gtgatcgagc tggccgacga ccggctgctg ccggagctgc tggcggggac
      661 atcgctgggt gagaagctga tctacacctt ctcgccccgg ctgatcgcca tcgacgccag
      721 cgccgccggt gagctggtgg ccgagctgac gcggctgggg cacgcgccgc ggctggtgga
      781 gggcgactga tggccgccga gaccccggcc aaggatattc tccggggcta cacgaacgcc
      841 gccctgcaga tgatggccaa gactcgcggg ctcaacgtgc agggcaaggg caaggagaag
      901 ctggttcagc ttctcgcgga gacactcttc agcccgagcc ggatcaagcc ggcactctcc
      961 gagttggagc cgttgcagcg cgaggtgctc gaccgggtga tcctggccgg cggcaggatg
     1021 ccgacgcagg tgctgcgcgc ggcgctggag caggagaacc tggtcgagcg gcagccgcca
     1081 cgcagcctct atggctacgc ccagccggtg tcgggctcgg cctacaagaa gggctcgcgc
     1141 aagttcgagg acagcctggc gcggctctgc gcgctcgggc tggtcttcac caccggggcg
     1201 tcgtcggggc gcatgtacga cctggtcacg cccgggctcc gggtctggat cccgccacag
     1261 atcttgcggc acctgccacc ggttacggtg gcgctggtca cgacggagcc accgccggag
     1321 atcgtcgcgg ggaaccccgg gccgctgcag cgcgatctct acacgctgct gagcctggcc
     1381 gagcgccaga cgatcccgct gacgatgcgc gggcaaatca tgaagcgggc gctggtcaag
     1441 ctcgatccgg agctactgct ctccgagatg accccgcaga cccgctccga ggatgagctg
     1501 ggccgcattc ccttcttgcg cgcgctggcc gaagacctcg gcctcctctc ggccagtgtc
     1561 ggcacgctgg cgccgaaccc cggcatccag gcctacctgc ggcgaccggc cggcgagcgc
     1621 ctggcgcagc tctaccagac ctatcgcgac agcgcgcgct tcaacgagct ctatcgcatc
     1681 cccaacctgc ggatcagcgg caagggaagc tcgacgcgcg ccgcgccgcc gccggtgatc
     1741 gcggcgcggc agcgcgtgct gcgcgagatc gccgaattgc cggccgagca gtggatcccc
     1801 ctcggccacc tgatcgatcg cctgcgccgg cgcgactacg agttcctgct ggagcgcccg
     1861 gatgaggtcc ggtacgagta ctactactac ggccgccaag acttcaatcc ctacgccaac
     1921 aacgccatgg gcgtgacgtt tgaggggctg aacgagggcg agggttggac ccgggtcgag
     1981 gccgggatga tccgggtgat cgtgaccgag gcactcgcct ggctgggcgt ggtcgatctc
     2041 ggctggcgcg agggcgagcc ggtagccttc cgcgtcagcg aggccggcgc ccacctgctg
     2101 cggggcgaga cgccggcctc gctggaggcc gcgcccacct ccaacgtggt ggtgcagccg
     2161 aacttccagc tcttcgcctt cgagccgacc ggcgaggatg ttctcttcat gctcgaccgg
     2221 atagccgagc gcgtgcgcgc cgagcaggcg atcgagtacg agctgacgcg cgactcgatc
     2281 taccgcggcc agcagagcgg gctcgatatc gcggagatca tcgcctttct ggagcgggtc
     2341 agtacggtgg agctgccgcg gaacgtgcgc cgcacgctgg ccgagtgggg cgcccagcac
     2401 gagcgcgtca ccctgcggcg cggcgtcggg ctgctgcacg cggtggacgc cgccacgctc
     2461 gacgcgctct acgccgaccc cgagcttggc ccgctgcttg ggcgccgcgc cgcgccggac
     2521 gtcgcgctgg tgccggccgc caacctgcac acgctcgtgc cgcgcctgct ggcggcgggg
     2581 cagttgccgg ccctgagcga aggggacgat ctggcgccgc gcccggtatt caccatcgac
     2641 cccgaggggc gtgtgacctt ccgccagcgg ctccccagca tccaggtgct gcgtgtcttg
     2701 cgtcccctga ccgatggccc gagccagccc ggccagggcg atacgccgcg cctgacccgg
     2761 gagagcctgc gccgggcggc gcggagcggg cagagcgccg aggcgatcat cgacagcctg
     2821 gcgcgcttcc acgacggtcc gctgccggag gaggtcgtgg ccttcgtcaa gcgctgggcc
     2881 aaggactggg gcaagggcgc gctggtcgag gcggtgctgc tgcagctcga gcgcccggag
     2941 ctgctggacg acctgctcgc cgaccccttg ctccggccct gcctgcgccg cgtgcccggc
     3001 gcgccggcct acgccctggt gcgccccggc gcgctggagg cggtgcgcgc ggcgctggag
     3061 gagcgcgaca tgccgctgca gggtgagt
//
LOCUS       DASUBI010000281         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_534136, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000281 DASUBI010000000
VERSION     DASUBI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534136"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFU72_05335"
     CDS             <1..592
                     /locus_tag="VFU72_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422942.1"
                     /translation="DSGEGEEDEDDETPAAKPAAPGGAYVLEFDAARKIAQGLGAHLD
                     RLPTVVAVKGDKARLLPVADRARHLFGKETAQPVVPTARTRRAAVQSALPGFPAQPHQ
                     GSLDDYIEQVVADAQADTAVPPAGSTVLDRLHQAMLLFAGGRTAGLRRFLVEEGIGRD
                     ERFWRLAQALSALYPMGSDEKRWVDGVLARKKGWGL"
     gene            594..3473
                     /locus_tag="VFU72_05340"
     CDS             594..3473
                     /locus_tag="VFU72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HEU5422943.1"
                     /translation="MPNMKPWHTVVTPREDLREKRPLDAAEFAVHLGLVREGTAPKVY
                     QQPQEFFERTFLTANLTALAAEVARRLSGIATETSAVFNLATQFGGGKTHALTLLYHL
                     AHNGVDRGTDWPGVGSILARAGVASIPRAAVATFVGTEFDSLDGRGGGDGSPHRVTPW
                     GEIAYQLGGAEAFAHVAEHDRQRIAPGGDVLRKILPTERPTLILMDELLNYAARWRRE
                     GVVSQLYPFLQNLSETARSLPNVVLAVSIPASELEMTPEDHADHERFKKLLDRLGKAY
                     LLSAETETSEIIRRRLFEWDLRTLGQNGRVNLPREARATCDDYALWVLDHRQQLPQWF
                     PLDRAAEAFAATYPFHPSVLSVFERKWQTLPRFQRTRGVLRLLALWVSHAYDDGYRKA
                     HRDPLIGLGTAPLEDPLFRAAVFEQLGESRLEPVVTTDICGKPGAHAVRLDEEAPELL
                     RKSRLHQKVATAIFFESHGGSAGSQPGASLPETRLAVAEPGLDLGNVETALDALVAAT
                     YYLRAEGTLYRFGLTPNLNKLLADRRATIQQVAIAERVRREIEAVFKAGSGAERVFFP
                     DKSNDIPDRPALTLAILAPDTLTLAEPDARERLLALTREYGASARQFKNALIWCVAES
                     SAALDEEARKVLAWESLDRDPHALGDDPNERQRLGHELSEGLGRAKRDLQNANWRAYR
                     RLLILDKANGLKDLDLGQLNASGGALVTQILAQLQQRDELTDGVGPAYLGRNWPPALP
                     EWSTRAVRDAFYASPLFPRLSRADVVKETIAKGVSEGVLAYVGAKSPSGEYRPFRFNE
                     PLSPTDVDLSADMFIIPAEAANAYLQAQSARTAQEAAEPTPVPVPVPIPAGASANDSG
                     IAIGADGQPAQEGTASFIYPRIGAQGGNPGVRDSRTLSWAGEIPPQKWTLFYTKVVSS
                     LVNAGELSLHLTLTVRLGDGASQQRIEALKAALRELGLSDTLDMS"
BASE COUNT          570 a         1299 c         1116 g          536 t
ORIGIN      
        1 cgattccggc gagggagaag aggacgagga cgacgagacg cccgccgcga agccggccgc
       61 gccgggcggc gcctacgtgc tcgaattcga cgccgcgcgc aagatcgccc agggactggg
      121 ggcgcacctc gaccgcctgc ccactgtcgt cgccgtcaag ggcgacaagg cgcggctgct
      181 gcccgtcgcc gaccgcgcgc gccacctctt cgggaaggag acggctcagc cagtcgtccc
      241 cacggcgcgc acacggcgtg cggccgtcca gtctgcgctc cccggtttcc ccgcgcagcc
      301 gcatcagggc tccctggacg actacatcga gcaggtcgtg gccgacgccc aggccgatac
      361 ggcggtgcct cccgcgggct ccacagttct cgaccggctg caccaggcga tgctgctctt
      421 cgccggtggc cgcacggcgg ggctgcggcg cttcctggtg gaagaaggca tcggccgcga
      481 cgagcgcttc tggcggctgg ctcaggcgct ctccgcactc tacccgatgg gcagcgacga
      541 gaagcgctgg gtggacggcg tgctggcgcg caagaaagga tggggactgt aacatgccga
      601 acatgaagcc ctggcacacc gtcgtcaccc cgcgcgagga cctccgcgag aagcgcccgc
      661 ttgacgccgc cgaattcgcc gtccatctgg ggctagtccg cgaggggacc gcgcccaagg
      721 tctaccagca gccacaagaa ttcttcgaac ggacctttct gaccgccaat ctcaccgcgc
      781 tggccgccga ggtcgcccgg cgcctctccg gcatcgccac ggagacttcg gccgtcttca
      841 acctcgccac ccagttcggc ggcggcaaga cacacgccct caccctgctc tatcacctcg
      901 cacacaacgg cgtggatcgc ggcacggact ggccgggtgt cggctcgatt ctcgcccgtg
      961 ccggcgttgc cagcatcccc cgcgcggcgg tggcgacctt cgtcggcacc gagttcgact
     1021 cgctggacgg gcgcggcggg ggagatggca gcccgcaccg tgtcacgccc tggggcgaga
     1081 tcgcctacca gctcggcgga gccgaggcct tcgcgcacgt ggccgagcac gaccgccagc
     1141 gcatcgcccc cggcggcgac gtgctgcgca agattctgcc caccgagcgc ccgacgctca
     1201 tcctcatgga cgagctgctc aactacgccg cgcgctggcg cagggaaggc gtcgtctcgc
     1261 agctctaccc cttcctacag aatctctccg agaccgcgcg ctcgctgcct aacgtcgtgc
     1321 tggccgtctc aatccccgcc tcggagctgg agatgactcc cgaggatcac gccgaccacg
     1381 agcgcttcaa gaagctgctc gaccggctcg gcaaggccta tctgctctcc gccgagaccg
     1441 agacctcgga gatcatccgc cggcggctct tcgagtggga tctgcgcacg ctgggacaga
     1501 acggccgcgt gaacctgccg cgcgaggcac gcgccacctg cgatgactac gcactatggg
     1561 tactcgacca ccggcaacag ctgccgcagt ggttcccgct cgaccgcgcc gccgaggcct
     1621 ttgccgccac gtatcccttc cacccctccg tgctgtcggt cttcgaacgc aagtggcaga
     1681 cgctgccgcg cttccagcgc acgcgcgggg tgctgcggct gctcgcgctg tgggtctcgc
     1741 acgcctacga cgacggctac cgcaaggcgc accgcgaccc cctgatcggc ctgggcaccg
     1801 ccccgctgga ggatccgctc ttccgcgctg cggtcttcga gcagcttggc gagtcgcgcc
     1861 tggaacccgt ggtgaccacc gacatctgcg gcaagccggg tgcgcatgcg gtgcgcctgg
     1921 acgaggaagc gcccgagctg ctgcgcaaga gccgcctaca ccagaaggtc gccaccgcca
     1981 tcttctttga gtcacacggc ggcagtgccg ggagtcagcc gggcgcgagc cttccggaga
     2041 cgcgcctggc tgtggccgag cctggtttgg acctgggcaa cgtcgagacc gcgctcgacg
     2101 cgctggtcgc cgccacctac tacctgcggg ccgaaggcac gctctatcgc ttcggcctca
     2161 cccccaacct caataagctg ctcgccgacc gccgcgccac catccagcag gtcgccattg
     2221 ctgagcgtgt gcggcgcgag atcgaggccg tcttcaaagc cggctcaggc gccgaacgcg
     2281 tcttcttccc tgacaagagc aacgacatcc ctgaccgtcc cgcacttacc ctcgccatcc
     2341 tcgcgcctga cacgcttacg ctcgccgagc ccgatgcccg cgagcggctg ctggcactca
     2401 cccgtgagta tggcgcctcc gcgcgccagt tcaagaacgc cctcatctgg tgcgtagccg
     2461 agtcttccgc tgcgctcgac gaagaggcgc ggaaggtgct ggcgtgggaa tcgctcgata
     2521 gggatccaca cgccctcggc gacgatccca acgaacggca gcgcctgggc cacgagctgt
     2581 ccgaaggtct gggtcgtgcc aagcgcgatc tgcagaacgc gaactggcgc gcctaccgcc
     2641 gactgctcat cctcgacaaa gccaacggcc tcaaagacct ggacctcggc cagttgaacg
     2701 cctctggagg tgcgctcgtc actcagatcc ttgcgcagct ccagcagcgc gacgagctga
     2761 ccgatggcgt cgggccggcc taccttgggc gtaactggcc cccggccctg ccagagtggt
     2821 ccacccgcgc cgtgcgagat gccttctacg cgtcgccgct ttttccgcgt ctctcgcgtg
     2881 ccgacgtcgt gaaggagacc atcgccaagg gagtgagcga aggggtgttg gcgtacgtgg
     2941 gcgcgaagtc ccccagcggc gaatatcggc ccttccgctt caacgagccg ctctccccca
     3001 ccgacgtaga cctctctgcc gacatgttca tcatccccgc ggaggccgcc aacgcctatt
     3061 tgcaggccca gtcggctcgt acggcccagg aagctgccga gccgactcct gttcccgtgc
     3121 cggtccccat ccccgccgga gccagcgcca acgactcagg gatcgccatt ggtgccgatg
     3181 ggcagccggc gcaagagggc accgcgtcct tcatctaccc caggatcggc gctcagggcg
     3241 gcaatccagg cgtgcgtgat tctaggaccc tgagctgggc cggcgagatt ccgccgcaga
     3301 agtggaccct cttctacacc aaggtcgtct cgtcgctagt caatgccggc gagctttctc
     3361 tacacctgac ccttacggta aggctcggcg acggcgcgag ccagcaacgg atcgaggcgc
     3421 tcaaggcagc gctacgcgag ctcgggctgt cagatacgct cgatatgtcg tgaccggcgg
     3481 cggatgatcc acatgaatag caccagcccg gcgaggccga c
//
LOCUS       DASUBI010000282         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_203055, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000282 DASUBI010000000
VERSION     DASUBI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203055"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="sbcD"
                     /locus_tag="VFU72_05345"
     CDS             complement(<1..548)
                     /gene="sbcD"
                     /locus_tag="VFU72_05345"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEU5422944.1"
                     /translation="MKIIHTSDWHLGRTLHGADLLPYQASFLDWLLGQAVSNQADAVV
                     VAGDIYDRAVPPTEAVTVLDRALRGFASARIPVLLISGNHDSAVRLGFGASLSEVAGV
                     HLRTDVADIARPVVLMDSYGEVAIYGIPYLLPDAVMTDPVLANHGALERSHESVLAAA
                     TAQIRADASARGIGRTVVTAHA"
     gene            538..1668
                     /locus_tag="VFU72_05350"
     CDS             538..1668
                     /locus_tag="VFU72_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5422945.1"
                     /translation="MIFIGGAPDVVGEEVLGRTPLHHAGPPRPFLGIGTGGGVSRASG
                     AAVLVLTLVTTSASQADKEEQHRLTTLSGLAALSLDTLSSVAYGPEAIVVVLVAAGSA
                     GLADALPVTYAIVILLAVLVISYRQVIEAFPSGGGAYAVARTHLGTMPSLVAAGSLIV
                     DYVLNAAVGVSAGVAALTSAFPGLYGATVPICLIVLALITAANLWGVAESARLFIVPT
                     LAFIFAIALVIVAGLVRSHPVVTPPSHLPSVAESVGALLLLRAFASGCSALTGVEAIA
                     NAVPSFRKPRAKHAQNTEAWLGALLGAMLIGLAILIKKYHVTPDPREVQPARPWQRIL
                     HNQRGFVLDRAIQRGTVNVVICRLHFQLANWPEPGATSGALH"
     gene            1655..2071
                     /locus_tag="VFU72_05355"
     CDS             1655..2071
                     /locus_tag="VFU72_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5422946.1"
                     /translation="MPSIEVDIARRLFSAARVARLVTVSASTPHVVPFTFAVDGDLIY
                     SAVDAKPKSTTNLRRLRNIRDNPRVAALADHYEDDWSALWWVRADGLAAVVSEAEAMA
                     RPLGLLAGRYPQYAASPPAGPVIVIRVDRWTGWTAS"
BASE COUNT          325 a          775 c          747 g          377 t
ORIGIN      
        1 aacgcgtgcg ccgtcaccac agtgcggccg atcccgcggg cggatgcgtc ggcccggatc
       61 tgagcggtcg cggccgccag gacggactcg tgcgagcgtt ctagcgcccc gtggttggct
      121 agcaccgggt cggtcatcac ggcgtcgggc agcaggtaag gaatgccgta gatggcgacc
      181 tcgccgtagg agtccatcag caccacaggc ctcgcgatgt cggccacgtc ggtgcgcagg
      241 tgcacgccgg ccacctcgct gaggctggcg ccgaacccca gcctgaccgc cgagtcatgg
      301 ttgccgctga tcagcagcac ggggatgcgg gcgctggcga acccgcgcaa cgcgcggtcg
      361 agcacggtga ccgcctcggt gggcggcacg gcacggtcgt agatgtcgcc ggccacgaca
      421 acggcgtcag cctgattgct caccgcctgg ccaagcagcc agtcaaggaa cgacgcctgg
      481 taaggcagca ggtcggcgcc gtgcaacgtc cgccccagat gccagtccga ggtatggatg
      541 atcttcatcg gcggtgcacc tgacgtcgtc ggggaagagg tgctcggtcg aactccgctc
      601 catcatgccg ggccgccgcg gccattcctg ggcattggca caggcggcgg cgtgtcgcgc
      661 gccagcgggg cggccgtgct tgtattgact ctcgtgacca cgagtgcaag tcaggcggat
      721 aaggaagaac agcacaggct gacgaccttg tcggggctgg ccgcgctgtc gctggacacg
      781 ctgtcctctg tggcatacgg gccagaggca atcgtggtcg tgcttgtcgc cgccgggagc
      841 gccggacttg ctgacgcgct gccggtgacg tacgcgatcg tgatcttgct ggccgtgctc
      901 gtgatttcct accgccaggt catcgaggcc tttcccagtg gcggcggtgc ctacgcggtc
      961 gcccgcacgc acctgggcac gatgccgagc ctggtcgcgg ctggctcgct gattgtcgac
     1021 tacgtgctga acgccgccgt cggcgtctcg gccggcgtcg cggcgctgac ctccgccttt
     1081 ccgggcctgt acggcgccac ggtgccgatc tgcctgattg tgctcgcgtt gatcaccgcg
     1141 gccaatctgt ggggcgtggc tgagtcggcg cggctcttca tcgtcccgac gctcgcgttc
     1201 atcttcgcga tcgcgctggt catcgtcgcc ggcctggtgc gcagccaccc ggtggtcacc
     1261 ccgccttcgc acctgcccag tgttgccgag tcggtcgggg cactgctgct gctccgcgcg
     1321 ttcgcttccg gctgctcggc cctgaccggt gttgaggcga tcgccaacgc ggtgccgtcg
     1381 ttccgcaagc cgcgagccaa gcacgcgcag aacaccgagg catggctggg cgccctgctc
     1441 ggcgccatgc tgatcggcct ggcgatcttg atcaagaagt accacgtcac gcccgaccca
     1501 cgcgaggtcc agccggccag gccctggcag cgcatcctgc acaaccagcg gggtttcgtc
     1561 ctggaccgcg ccattcagcg cggcaccgtg aacgtggtga tctgccgcct ccacttccag
     1621 ctagccaact ggccagaacc gggagcgacc tccggtgccc tccattgaag ttgatatcgc
     1681 tcggcgcctg ttttctgccg ctcgggtagc ccgactcgtt accgtttccg cgagcacgcc
     1741 gcacgtagtc ccgttcacgt tcgccgtcga tggcgacctg atctactccg cggtcgacgc
     1801 caaaccgaag tccacgacca atctgcgacg actgcggaac attcgcgaca atccccgcgt
     1861 ggcggcgctc gccgaccatt acgaggacga ctggtcggcg ctttggtggg tgcgcgccga
     1921 cggactcgcc gcggtcgtct cagaggccga agccatggcg cggccgctcg gcctgctggc
     1981 cgggcgctac ccccagtacg cggccagccc gccagccggg ccggttatcg tgatccgggt
     2041 cgaccggtgg acgggctgga ccgcgagcta gcaggctaca agccggccac ctgcgggatc
     2101 acctgagagc cgatgacctc aagcggcgtc acgtcccaga cattggccac ggcgccgatg
     2161 gcggtgtcga aacccatctc ggcgagccgg cctagctgat cgacaatctc gcctgccttc
     2221 tcgc
//
LOCUS       DASUBI010000283         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_533520, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000283 DASUBI010000000
VERSION     DASUBI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533520"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFU72_05360"
     CDS             complement(<1..698)
                     /locus_tag="VFU72_05360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5422947.1"
                     /translation="MSGLYDELTIFSGNSNPALTREVCSCIDLPPGRIEVFTFSNDNI
                     FVRIGESVRENDVFVIQSLSYPVNHHIMELLIILDTLKRASAGRITAVIPYYAYGRTD
                     KKDQPRVPITARLIADLITVSGADRVLTVDLHAGQIQGFFNIPVDEMTAMGILAEHFR
                     EKGLTNPVVVSPDLGNTKRARNFAEALNASLAIIEKRRLGNVDQTEVLNLIGSVAGGQ
                     AVLIDDEIDTAGSIT"
     gene            complement(682..1398)
                     /gene="rnc"
                     /locus_tag="VFU72_05365"
     CDS             complement(682..1398)
                     /gene="rnc"
                     /locus_tag="VFU72_05365"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5422948.1"
                     /translation="MDRAETNLGIEFRDRELLRLALIHRSYLNEHDVAVEAREPNERL
                     EFLGDSVLGMVTAEFLYKRYPRLPEGELTAYRTALVRTETLAGWARRFGLDALLYLGR
                     GEQSDGDIPNRILAGAFEAVLAAIFLDRGMRVTRRFLWGLLKADAERIIRAGQETNYK
                     GRLQELIQERERITPAYRTVAVEGPAHERTFTVEVLLNGRPIGHGSGNSKRVAQQLAA
                     KSALIALGEEGIIPSDERPL"
     gene            complement(1452..1892)
                     /locus_tag="VFU72_05370"
     CDS             complement(1452..1892)
                     /locus_tag="VFU72_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422949.1"
                     /translation="MSVPDDLDRLLKYIAQQYTGRARAKEVERDAAAEEPDTALAASE
                     RKTIAAEERRAESMAADFRDGLQRAIAARRAGGNAISLDDRVPEENRMADALVHFLVG
                     AGLADSTTREIVPLHYVYTIAIDWKQLAEVAAAAHVNLDTLLGQ"
     gene            complement(1917..2153)
                     /locus_tag="VFU72_05375"
     CDS             complement(1917..2153)
                     /locus_tag="VFU72_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422950.1"
                     /translation="MSGELKITTEDGKNYWFGKDERFPGEVRIYGAANQDHWAYMPTN
                     QARQIVRQGLEHGGEVQQGDPVLFFDRAEHTPAG"
     gene            2275..>2815
                     /locus_tag="VFU72_05380"
     CDS             2275..>2815
                     /locus_tag="VFU72_05380"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEU5422951.1"
                     /translation="MTEFAPLEYRDGYLPIADHGLIGDGTTAALIARDGAVVWLCLPR
                     FDSPPVFCHILDHERGGVFRVAPEGVVAARQRYEPDTAVLVTELRTPGGLVRLTDALV
                     LRAGARLEEDAAADRGELVRLVEVLQGPVTLQVDLEPRGGGEIRGRSEALCLRPAGRA
                     DIELQLSSSLAISEPHTSLE"
BASE COUNT          410 a          960 c          953 g          492 t
ORIGIN      
        1 gtgatcgagc cggcggtgtc gatctcgtcg tcgatcagca cggcctggcc ccccgcaacc
       61 gaaccgatca gattcagcac ctcggtctgg tcgacgttcc ccagccggcg cttctcgatg
      121 atcgccagcg aggcgttcag cgcctcggcg aagttgcgcg cccgcttggt gttgcccaga
      181 tccggtgaga cgacgaccgg attcgtgagc cccttctcgc gaaagtgctc ggccagaatc
      241 cccatggcgg tcatctcatc caccgggata ttgaagaagc cctggatctg gccggcgtgg
      301 agatcgaccg tcagcacccg atcggcgccg gagacggtga tcaggtcggc gatcaggcgc
      361 gccgtgatcg gcacgcgcgg ctggtccttc ttatcggtgc ggccgtaggc atagtaggga
      421 atcacggcgg tgatgcgccc ggccgaggcg cgtttgagcg tgtcgaggat gatcagcagc
      481 tccatgatgt ggtggttgac cgggtagctg agcgactgga tgacgaagac gtcgttctcg
      541 cgcacgctct cgccgatgcg cacgaagatg ttgtcgttgc tgaaggtgaa gacctcgata
      601 cggccgggcg gcaggtcgat gcaggagcag acctcgcggg tcagagccgg gttcgagttg
      661 ccgctgaaga tggtcagctc atcatagagg ccgctcatcg ctcgggatga ttccttcctc
      721 gcccagcgcg atcaaggcgc tcttggccgc caactgctgt gccacgcgct tgctgttgcc
      781 gctcccgtga ccgatcggcc gcccgttgag cagcacctcg acggtgaacg tccgctcgtg
      841 ggccgggccc tccaccgcca cggtgcggta ggccggcgtg atccgctcac gctcctggat
      901 cagctcctgc aggcggccct tgtagttggt ctcctgcccg gctctgatga tgcgctcggc
      961 gtcggctttg agcaagcccc agaggaagcg ccgcgtgacg cgcatgccgc gatcgagaaa
     1021 gatcgcggcc aggaccgcct cgaaggcgcc ggccagaatg cggttgggga tatcgccgtc
     1081 gctctgctca ccccggccga gatagagtag cgcgtcgagg ccgaagcgac gcgcccagcc
     1141 ggccagcgtc tcggtgcgca ccagcgcggt acggtaggca gtcagctcgc cctccggcag
     1201 gcgcgggtag cgcttgtaga ggaactcggc ggtcaccatg ccgagcaccg agtcgcccag
     1261 gaattccagg cgctcgttcg gctcgcgggc ctccaccgcc acgtcgtgct cgttgagata
     1321 ggagcggtgg atcagcgcca ggcgcagtag ctcgcgatcg cggaactcga tgcccaggtt
     1381 ggtctcggcg cgatccaacc ggcgctctgg gtcgagccca cccttcgcgc gggattcggg
     1441 tgcgacagcc atcactgccc gaggagcgtg tcgaggttga cgtgcgcggc cgccgcgacc
     1501 tcggccaact gcttccagtc gatcgcgatg gtgtagacgt agtgcaacgg cacgatctcg
     1561 cgcgtggtcg agtcggccag gccggcgccg accaggaaat gcaccagcgc gtcggccatg
     1621 cggttctcct cgggcacgcg gtcgtcgagc gagatggcgt tgccgccggc ccggcgcgcg
     1681 gcgatcgcgc gctgcaggcc atcgcgaaag tcggcggcca tcgactcggc gcgacgctcc
     1741 tcggccgcga tcgtcttgcg ctcgctggcg gccagggccg tgtccggctc ctcggccgcg
     1801 gcgtcgcgct cgacctcctt ggctcgcgcc cgcccggtat attgctgggc gatgtatttc
     1861 agcaatcgat ccagatcgtc cggcacgctc attcctcgcc ttgtcccgac ccctctctac
     1921 cccgcgggcg tgtgctccgc gcgatcgaag aatagcactg gatcgccctg ctgcacctcg
     1981 ccgccgtgct cgagcccctg ccgtacgatc tgccgggcct ggttggtcgg catataggcc
     2041 caatgatcct ggttggcggc gccgtagatc cgcacctcgc cggggaagcg ttcgtcctta
     2101 ccgaaccagt agttcttgcc gtcctcggtc gtgatcttca attctccgct caacctgctc
     2161 tccctccgac tcagaagaaa cggccgtcca ggtggcacga tcgcaggacg tctgccctgg
     2221 ctccacgctt gcgtgctacc atcggctgtt gactcgtgcc gcctgaggag gccgatgacc
     2281 gaattcgccc cgctcgagta ccgagatggc tatctcccga tcgcggatca cggcctgatc
     2341 ggtgacggca ccaccgccgc cctgatcgcg cgcgacggcg ccgtcgtctg gctctgcctg
     2401 ccgcgcttcg actcgcctcc ggtcttctgt cacatcctcg accacgaacg cggtggtgtc
     2461 ttccgggtcg ctccggaagg agtcgtcgcc gcccgccagc gctacgagcc ggacaccgcc
     2521 gtgctggtga ccgagctgcg cacgcccggc ggcctggtgc gcctgactga tgcgctggtg
     2581 ctgcgggccg gagcgcggct ggaggaggat gccgcggccg accggggcga gctggtgcgg
     2641 ctggtcgagg ttctccaggg accggtgacg ctacaggttg acctggagcc gcgcggcggt
     2701 ggcgagatcc gcggccgcag cgaggcgctc tgcctccgcc ccgccgggcg cgcggacatc
     2761 gagctgcagc tgagcagcag tctggcgatc agcgagccgc acacgagcct ggagc
//
LOCUS       DASUBI010000284         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_697486, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000284 DASUBI010000000
VERSION     DASUBI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697486"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..683
                     /locus_tag="VFU72_05385"
     CDS             138..683
                     /locus_tag="VFU72_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422952.1"
                     /translation="MSATNDETLRRVSQPTYQNRLRVIGRQLDLGRFRAINLLEIGGG
                     FLVRALTSSNRLAEALEFPHSEFRDSVARAIAARGEGERQHPAHPLLPTGYEDFLRAL
                     GYRLDEQHAEAIAVTELEGFIAVSGVAKRDRISQTSIAPFQTLLRADDIVALLDEAFR
                     RRGAPVPEPKPRQGLARRFRP"
     gene            784..1395
                     /locus_tag="VFU72_05390"
     CDS             784..1395
                     /locus_tag="VFU72_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422953.1"
                     /translation="MTSSNQPEQPAHGDRTVVAVFDRHAGVERAIEALRAAGYTNQEI
                     TLVARNTVAGQEIPNIAASEEAVKQSGIGAAIGGVSGTALAFLGAGTALAIPGIGPVL
                     LALGGLAVGAGAGAWIGSILGLEVPDDVARRYSDLANQGHLILFVRPFKGQHDQVEQL
                     LRQAGSNDVQSYQYTFQVSNVDQLPGASPHTQAEPSDEPGETA"
     gene            complement(1565..2374)
                     /locus_tag="VFU72_05395"
     CDS             complement(1565..2374)
                     /locus_tag="VFU72_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5422954.1"
                     /translation="MRRDRPLIIAHRGGSPRDIDNSREAFVHALAVGVDMLECDLRRT
                     RDGALVLLNAEAIRSDGRRLVVADTSYVDLKRALPWLLTLAEFLAEFGGALPFNLDLK
                     THGYELAALAEIERHGLTERVIFSTGHTLSLRRLAAARPGLRLGLSRGHLNSHRSLRW
                     LTFWLQWLLPRLLPLMLRAADATAAMLQYRVADRALVERLHRFDYEVFSWTVDTPAEA
                     FRLTDAGVDGIATNVPARLLASFAEREEHQRALGIDKDGTLVRTDPSTSSG"
     gene            complement(2371..3120)
                     /locus_tag="VFU72_05400"
     CDS             complement(2371..3120)
                     /locus_tag="VFU72_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5422955.1"
                     /translation="MAKIAHELEMAAASAEHAASQRPTTPIPVQRRRGRAFLIAALVA
                     FGGYLVLLVLVRFNRQLRLDIDATLHFQRRRHPLLARLMTAVSWFGFRPQSFLLPSAA
                     VGALWVLRFRLESVFLGLAWLASFGNFLTKLVVNRPRPQGFGIRVAPAKILHSSFPSG
                     HVLHYLAFWGFLSYIVFTKATSPIRWAVLAVVGVFVSLVGASRVYLGHHWLTDVLASY
                     LLGFAYLIGLIAFYRRVRARTLRDRGPGAPR"
     gene            complement(3421..4371)
                     /gene="ispH"
                     /locus_tag="VFU72_05405"
     CDS             complement(3421..4371)
                     /gene="ispH"
                     /locus_tag="VFU72_05405"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5422956.1"
                     /translation="MSKQIVLAEEMGYCWGVRRALDIITKAGSTAAPVATVGDVIHNP
                     QIVERLRSHGVDTAETVAEAETRGFQRVAITAHGAGPERAADASARGLELIDTTCPLV
                     TKVQRLAQKLVRQGYYLVVYGDAPHPEVRGVIAWAGTSRAVAAKHIEQLPWTAARGQG
                     EPSQVPPRKVAVVSQTTKNVDEFLTFIGELTPLVARDGGELRVCNTICEPTTERQNAL
                     RRLAAEVDAIVVIGGKKSSNTARLAEVGRSLGVPSYHVERPEEVDPAWLEDVERVGVT
                     AGASTPDDVILELVHHLETLGFDAPEEELAVRDLNATPAF"
     gene            complement(4402..>5229)
                     /locus_tag="VFU72_05410"
     CDS             complement(4402..>5229)
                     /locus_tag="VFU72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5422957.1"
                     /translation="PEAFRADLQWLYDHDFYVIPIHDLISNSIKAPAGKRPVVLTFDD
                     GTVTQFRYIVAADGSKTIDPTCAVGILEDFFGKHPDFGRGGLFSIIATEPFAWPAADD
                     QQPYMKEKVQWLIDHGYEIGNHTVHHADMKQETDEQIEAELAGAVDVMRDFSPQAQME
                     VIAVPFGSYPKGGDTTLFAGFDYRGKHYGAKAALEVGANPGPSPMDKDWDPMWIPRIR
                     GTTDELDKWFAWAQDNPGILYVSDGNPDTITIPRDLPPALAGKLDEARAAGKPIIRY"
BASE COUNT          829 a         1778 c         1826 g          796 t
ORIGIN      
        1 atcatgcggg cctacgagct tggtgcaagc ggctatatca gcaagccaag ctatctccac
       61 gacctgcgcg ccgtgttggg caacacgctt ctctactggt cggccatgaa gcggccgagc
      121 gaagggagcg agaccgcgtg agcgcgacga acgacgagac gctgcggcga gtctcacagc
      181 ccacctacca gaaccggctc cgtgtcatcg ggcggcagct cgacctcggc cgctttcgcg
      241 cgatcaacct cctggagatc ggcggaggct tcctcgtccg cgccctcacc tccagcaatc
      301 gcctggcgga agccctggaa tttccccaca gcgagttccg agatagtgtc gcgcgggcga
      361 tcgccgcgcg cggtgagggc gagcggcaac acccggcgca tccgctgcta cccacgggct
      421 atgaggattt cctgcgcgcg ctcggctacc gcctggacga gcagcacgcg gaggcgatcg
      481 ccgtgaccga gctggaaggc ttcatcgcgg tgagcggagt ggccaagcgc gaccggattt
      541 cccagacgag catcgcgccc ttccagacgc tgctgcgtgc ggacgacatc gtcgcgctgc
      601 tcgacgaggc atttcgccgg cgcggagccc ccgtccccga gcccaagccg cgccaggggc
      661 tcgcccggag gttccggccc tgatgtcgcc cgccggggag cggcgcgcgt gcggggcacg
      721 agccttgcat cggtatgccg ggcgacgacc cgccgcccca gggcgcagcg acggagaatg
      781 ccaatgacaa gcagcaacca gccagagcag cccgcgcacg gcgaccgcac cgtggtcgcc
      841 gtcttcgacc gccacgccgg cgtcgagcgc gcgatcgagg ccctgcgtgc ggccggctat
      901 accaaccagg agatcacgct ggtggcgcgc aacacggtgg ccggccaaga gatccccaac
      961 atcgccgcca gcgaggaggc ggtgaagcaa tccggcatcg gcgcggccat cggcggggtg
     1021 agtgggacgg ccctggcctt cctcggcgcc ggcacggcgc tcgcgatccc cggcatcggg
     1081 ccggtcttgc tggcgcttgg cgggctggcg gtcggggcgg gagccggcgc ctggatcggc
     1141 agcatcctgg ggcttgaagt gccggacgac gtcgcccggc gctacagcga cctggcgaac
     1201 caggggcact tgatcctctt cgtgcgcccc ttcaagggcc agcacgacca ggtcgagcag
     1261 ctgctgcgcc aggccggctc gaacgacgtg cagagctacc agtacacgtt ccaggtcagc
     1321 aacgtcgatc aactgcccgg cgccagcccg cacacgcagg ccgagcccag cgacgagccg
     1381 ggggagacgg cgtaggcggt gttctcgtgg ctccgctgac tggcaccaag gcattgcatc
     1441 gatcgcggac ggatccttcg actgcgctca gagcctgccc tgagcctgcc gaagggatga
     1501 caatgtttgg ttagagacct ttcggacgag gcaattcctc gtaggaagtc cctctcgcac
     1561 ccggtcatcc tgagcttgtc gaaggatccg tcctcacgag cgtcccatcc ttgtcgatgc
     1621 ccagtgccct ttggtgctct tcccgctcgg cgaacgaggc cagcaggcgc gcggggacgt
     1681 tggtggcgat gccgtcgacg cccgcgtcgg tcaggcggaa ggcctcggcg ggtgtatcca
     1741 ccgtccagct gaacacctcg tagtcgaacc ggtgcagccg ctcgaccagg gcgcggtcgg
     1801 cgacgcggta ctgcagcatg gcggcggttg cgtccgccgc gcgcagcatg agcggcaaga
     1861 ggcgcggcag cagccactgg agccagaagg tgagccagcg cagtgagcgg tggctgttga
     1921 ggtggccgcg cgagagcccc aggcgcaggc ccggccgcgc ggcggccaac cggcgcaggc
     1981 tgagggtgtg ccccgtggag aagatcaccc gctccgtcaa cccgtggcgc tcgatctcgg
     2041 ccagcgcggc cagctcgtag ccgtgggtct tgagatcgag gttgaagggc agcgcgccgc
     2101 cgaactccgc caggaactcg gccagcgtca gcagccaggg gagcgcccgc tttaggtcga
     2161 cataactcgt atcggccacg accaatctcc ggccgtcact ccggatcgcc tcagcgttga
     2221 gcagaaccag cgcgccgtcg cgcgtgcggc gcaggtcgca ctccagcatg tcgacgccga
     2281 cggccagggc gtggacgaac gcctcgcgcg agttgtcgat atcgcgcggc gagccaccgc
     2341 ggtgggcgat gatcaggggg cggtcgcgtc tcaccgcggc gcgcccgggc cgcgatcacg
     2401 aagcgtgcgc gcgcgcacgc gccggtagaa cgcgatcagg ccgatcaggt aggcgaagcc
     2461 gaggaggtag gaggcgagca cgtcggttag ccagtggtgg ccgagataga cgcgcgaggc
     2521 gccgaccagc gacacgaaga cgccgacgac cgccagcacc gcccagcgga tcggactggt
     2581 cgccttggtg aagacgatgt agctcaggaa gccccagaag gcgaggtagt gcagcacgtg
     2641 accgctgggg aagctgctgt gcaggatctt ggccggcgcc acccggatgc cgaagccctg
     2701 cgggcgcggg cggttgacca ccagcttggt caggaagttg ccgaagctcg ccagccaggc
     2761 gagcccgagg aagacgctct ccagccggaa gcgcagcacc cagagcgcgc ccacggccgc
     2821 ggacggcagc aggaagctct gcgggcggaa gccgaaccag ctcacggcgg tcatcagccg
     2881 cgccagcagt gggtggcggc ggcgctggaa gtggagcgtg gcgtcgatgt ccagccggag
     2941 ctggcggttg aagcgcacca gcaccagcag caccaggtag ccgccgaagg cgaccagcgc
     3001 ggcgatcaag aaggcgcgcc cgcgccgccg ctgcaccggg atcggcgtgg tcgggcgttg
     3061 gctcgctgcg tgctcggcgc ttgcggccgc catctccagt tcgtgggcta tctttgccat
     3121 gggcgctcct ctggacttcg cttgtccacc cgtgcaacac ccgcaagagc cggcccacgt
     3181 ttcggggccc tcacccccgg cccttctccc gcgtaccggg tgaggggagg tggcgaacgc
     3241 caagaatggt tgccgcgtca gcacggatcc ttcgacaagc tcaggatgac agggaaagga
     3301 agcagcctac cggacgaggc acgtcctcct cgtcggcctc tcacgaaccc tgtcatcccg
     3361 accgaagcgg agggatcctt gctgacgcgg caacctatgt caccgatggg cacaacccgc
     3421 ctagaacgcc ggcgtagcgt tcaggtcgcg gacggccaac tcctcctcgg gggcgtcgaa
     3481 gccgagtgtt tcgaggtggt gcaccagctc cagaatgacg tcgtcggggg tcgaggcgcc
     3541 cgccgtcacg cccacgcgct cgacatcctc caaccaggcc gggtcgacct cctcgggccg
     3601 ctcgacgtgg tagctcggca cgcccaggct gcggccgacc tcggccaggc gggcggtgtt
     3661 cgagctcttc ttgccgccga tcaccacgat cgcgtccacc tcggcggcca ggcgccgcag
     3721 cgcgttctgc cgctcggtcg tcggttcgca gatcgtgttg cagacgcgca gctcgccgcc
     3781 gtcgcgcgcc accagcggcg tcagctcgcc gatgaaggtc agaaactcat ccacgttctt
     3841 ggtcgtctgg ctgaccaccg ccaccttgcg cggcggcacc tgacttggct ctccctggcc
     3901 gcgcgccgcc gtccagggca gctgctcgat gtgcttggcg gccaccgcgc gcgaggtacc
     3961 ggcccaggcg atcacaccgc gtacctccgg gtggggcgcg tcgccgtaga ccaccagata
     4021 gtagccctga cgcaccagct tctgggccag gcgctgcacc ttggtcacca gcgggcaggt
     4081 ggtgtcgatc agctccaggc cgcgggcgct ggcatcggcc gcccgctctg gcccggcgcc
     4141 gtgggcggtg atcgccaccc gctggaagcc gcgcgtctcg gcctcggcca cggtctccgc
     4201 cgtgtcgacg ccgtgcgacc tcagccgctc gacgatctgg gggttgtgga tcacgtcgcc
     4261 gacggtggcg accggcgccg cggttgagcc ggctttggtg atgatgtcga gcgcgcggcg
     4321 cactccccag caatagccca tctcttcggc caggacgatc tgcttgctca tggtctgcgt
     4381 tccctgctca gctcgacacg atcagtagcg gataatcggc ttcccagcgg cgcgcgcctc
     4441 gtcgagcttg ccggccagcg cgggcgggag atcgcgcggg atcgtgatcg tgtccgggtt
     4501 cccgtcgctg acgtagagta taccggggtt gtcttgcgcc caggcgaacc atttatccaa
     4561 ttcgtcggtt gtgccgcgaa tgcgcgggat ccacatcggg tcccagtctt tgtccatggg
     4621 cgaggggccg gggttggcgc cgacctcgag cgccgctttg gcgccgtagt gcttgccgcg
     4681 gtagtcaaag cctgcaaaca gcgtggtgtc gccccccttg gggtagctgc cgaagggcac
     4741 cgcgatgact tccatctgcg cctggggcga gaaatcccgc atcacgtcga ccgcgcccgc
     4801 cagctcggcc tcgatctgct cgtcggtctc ctgcttcatg tctgcatggt gcacggtgtg
     4861 gttgccgatc tcgtagccgt gatcgatcag ccactgcacc ttctctttca tgtacggctg
     4921 ctggtcgtcg gcggcgggcc aggcgaaggg ctcggtcgcg atgatcgaga agaggccgcc
     4981 gcggccgaag tcggggtgct tgccgaagaa gtcctccagg ataccgacgg cgcaggtggg
     5041 atcgatcgtc ttactgccgt cggccgccac gatgtagcgg aactgggtga cggtgccgtc
     5101 gtcgaaggtc agcaccacgg gtcgcttgcc ggccggagcc ttgatgctgt tgctgatcag
     5161 gtcgtggatc gggatgacgt agaagtcgtg gtcgtagagc cactgaaggt cggcgcggaa
     5221 ggcctccgg
//
LOCUS       DASUBI010000285         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_808730, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000285 DASUBI010000000
VERSION     DASUBI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808730"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFU72_05415"
     CDS             complement(<1..205)
                     /locus_tag="VFU72_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422958.1"
                     /translation="MSEIVHEAGKEPFDIDAAMDVVAEAVEPYPPAALFQLADEGFGS
                     PFEQLVACMISIRTLDETTLVVGR"
     gene            295..978
                     /gene="nfi"
                     /locus_tag="VFU72_05420"
     CDS             295..978
                     /gene="nfi"
                     /locus_tag="VFU72_05420"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476949.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEU5422959.1"
                     /translation="MRGRQGRGEVPPQVIDEYRERQREMAARVEFTPLEAPPRLIAAV
                     DVHLRDDTGIAAVVLMRYPELEVVEEQVASTPVDFPYISGLLTFREAPACLAALGQLH
                     EQPDLLLIDGQGRAHPRRFGLACHIGVALDRPTIGVAKSVLTGRYAELGEARGSSAEL
                     VSRGEVVGMALRSRDGVTPLYISVGNRITLPEAVEWTLRCCTRYRLPEPSRLAHRLAK
                     RLATETTGP"
     gene            complement(1196..1873)
                     /locus_tag="VFU72_05425"
     CDS             complement(1196..1873)
                     /locus_tag="VFU72_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEU5422960.1"
                     /translation="MKISLRSELPQIVLIAVMFALAAITWPSAPDRIATHWNLHGQVD
                     GYGGKFSGLLLLPLLALGLYLLLLLLPLLDPGRANYRNFARAYTIIRLAVLVLLAAIY
                     GVTILWIHGIELDMARVVPVLLGLLFIAIGSVLGKVRPNWFVGIRTPWTLSSKRSWVK
                     THRLGGYLFVLAGFTLVVAGLITSTALFPVVFGLLIALTLVLVAYSYVQWRRDPEKQP
                     PSGSLPG"
     gene            complement(1851..2150)
                     /locus_tag="VFU72_05430"
     CDS             complement(1851..2150)
                     /locus_tag="VFU72_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5422961.1"
                     /translation="MDEVFRALSDPKRRAILRLLRQRDFSAGELGERFPVARSTLSGH
                     LSVLRAAGLVVTERQGTTIVYSLNLAVYEELLAVVLELLGVGAREGARHEDQSPQ"
     gene            2377..>2914
                     /locus_tag="VFU72_05435"
     CDS             2377..>2914
                     /locus_tag="VFU72_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422962.1"
                     /translation="MTRDSIGSAQYQVTGVILAHIEEIGGLWKRLLGYQPKDPGAAAV
                     DALTDRSLLAFATYLRGDDSGRALLSQQWLALRPDQRMAADAVAAMSLFPEAVRQVLA
                     DRPELPLPLAEILAAAREFVLRTTTAVLQSIDLEPSEARWEEAARELEEQRDRRVARL
                     GVLTEIARAVNTSDDLDSL"
BASE COUNT          484 a          971 c         1040 g          419 t
ORIGIN      
        1 gccggccgac caccagggtc gtctcatcga gcgtgcggat cgagatcatg caggcgacca
       61 gttgctcgaa gggcgagccg aagccctcgt cggcgagctg aaagagcgcg gccggtgggt
      121 agggttcgac cgcctcggcg acgacgtcca tggccgcgtc gatgtcgaaa ggttccttgc
      181 cggcctcgtg cacgatctcg gacaacggcg cacctcccag atccttgctg ccgattgtaa
      241 gccagatcga gtcgcgggca cgccgcgtgc tgccggcagg ggcaggaggt gagcatgcgc
      301 ggccggcagg gtagaggcga ggtgccgccc caggtaatcg acgaataccg cgagcggcag
      361 cgggagatgg cggcgcgggt ggagtttacg ccgctggagg cgccgccgcg cctgatcgcg
      421 gcggtcgacg tccacctgcg cgacgacacc ggcatcgccg cggtggtgct gatgcgctac
      481 cccgagctgg aggtggttga ggagcaggtc gccagtacgc cggtcgactt cccctacatc
      541 tccggcctcc tcactttccg cgaggcgccc gcctgcctgg cggcgctggg gcagttgcac
      601 gagcagcccg atctgctgct gatcgacggg caggggcgcg cgcacccacg ccgcttcggg
      661 ctggcctgcc atatcggggt cgcgctggac cgcccgacca tcggggtggc caagtcggtc
      721 ctgaccgggc gctacgccga gctgggtgag gcgcgcggca gcagcgccga actggtctcg
      781 cgcggcgagg tggtgggcat ggcgctgcgc agccgcgacg gtgtcacgcc gctgtacatc
      841 tcggtcggca accggatcac gctgccggag gcggtggagt ggacgctgcg ctgctgcacc
      901 cgctaccggc tgcccgagcc ctcgcgcctg gcgcaccggc tggccaagcg cctggcgacg
      961 gaaacaaccg ggccctaacc cgtggagcgg tatacatggt cctcggccgg cgcggttcag
     1021 ttcctgctgt aggggcagac ctccgtgtct gccctcgccg ccctgccgct ggacgttgtc
     1081 gatcgctgcg atgtcgtccc gattcccgtt gccggggcgt ggggcaggca gggcagacac
     1141 agaagtctgc ccctacaatt gacgaggcac cggagccaac ggacgggccg accccctacc
     1201 ccggcagcga gccgctggga ggctgcttct cggggtcgcg gcgccattgg acgtaggagt
     1261 aggccaccag caccagcgtc agcgcgatca gcaggccgaa gacgacgggg aagagggcgg
     1321 tgctggtgat cagaccagcc acgaccagag tgaatccggc cagcacgaag aggtagccgc
     1381 ccagacggtg ggtcttgacc caggagcgct tgctcgatag ggtccagggg gtgcggatgc
     1441 cgacgaacca gttgggccgc accttgccca gcacgctgcc gatggcgatg aagagcagac
     1501 cgagcagcac cggcacgacg cgcgccatgt cgagctcgat accgtggatc cagaggatgg
     1561 tgacgccgta gatcgccgcc agcagtacga gcaccgccag ccggatgatg gtgtaggccc
     1621 gcgcgaagtt gcggtagttg gcgcggcccg ggtcgagcag cggcagcagc agtaggagaa
     1681 ggtagaggcc cagcgcgagc agcggcagca gcagcagccc gctaaacttg ccgccgtagc
     1741 cgtcgacctg gccgtggagg ttccagtgcg tggcgatgcg gtcgggcgcc gagggccagg
     1801 tgatggccgc cagcgcgaac atcaccgcga tcagcacgat ctgtggcaac tcactgcgga
     1861 gactgatctt catggcgcgc tccttctcga gcaccgacac ccagcagctc cagcacgacc
     1921 gccaggagct cctcgtagac cgccaggttg aggctgtaga cgatcgtcgt gccctgtcgc
     1981 tcggtcacca ccagccccgc cgcgcgcagc acgctcaggt ggcccgagag ggtggagcgc
     2041 gccaccggga agcgctcgcc cagctctccg gcactgaagt cgcgctggcg cagcaggcgc
     2101 aggatcgccc gccgcttggg atcgctcaac gcccggaaaa cctcgtccac tcgctcgcca
     2161 cctcacggcc gtatttcggc aaagaccgaa ataagtatag ccccgcgccg ctgccgtacc
     2221 aagtgtcgcg ccggtatcct gtaccaatgg acagggcgcg actgtgcgca gggccgattc
     2281 gttcgcaccg cgaaatgcaa atactcaaag cagagggaac ggcaagagga tgccgtttgg
     2341 ggagcacgag tctgttgggg gtcggtggag agtatgatga cgcgtgacag catcggcagc
     2401 gcacagtacc aggtcacggg ggtgatcctc gcccatatcg aggagattgg gggcctctgg
     2461 aagaggctcc ttggctatca gccgaaagac cccggcgcgg ctgctgtcga cgccctcacc
     2521 gaccgctcgc tgctcgcctt cgccacctat ctgcgcggcg acgacagcgg gcgcgcgctc
     2581 ctctcccagc agtggctggc attgcggccc gatcagcgca tggcggccga cgccgtggcg
     2641 gcgatgagcc tcttccccga ggccgtgcgc caggtgctcg ccgatcggcc ggagctgccg
     2701 ctgccgctcg ccgagatcct ggccgccgcg cgcgagttcg tgctgcgtac caccaccgcc
     2761 gttctgcagt cgatcgatct ggagccgagc gaggcccgct gggaggaggc ggcgcgcgag
     2821 ctggaggagc agcgcgaccg gcgcgtggcg cggctcggcg tgctgaccga gatcgcccgg
     2881 gccgtcaata ccagcgatga cctggacagc ctct
//
LOCUS       DASUBI010000286         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_636237, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000286 DASUBI010000000
VERSION     DASUBI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636237"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..647)
                     /locus_tag="VFU72_05440"
     CDS             complement(234..647)
                     /locus_tag="VFU72_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422963.1"
                     /translation="MSIDAGAESGVTAELRALRERMLPMLRAITREYVTRSDPGYPVV
                     VDNLAAGYFGLTLDPQYGLYFMNDGERVIAQLNIVGWRSDVRSSASKEKFASLPFSGV
                     RPVSNALSDNQLRNLLAELLSHWNKQPLMIRVTDS"
     gene            complement(644..1327)
                     /locus_tag="VFU72_05445"
     CDS             complement(644..1327)
                     /locus_tag="VFU72_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEU5422964.1"
                     /translation="MDHSSTLRLRDMPRWSGDQWLTGAWRFVMLIAGLFIFATGLTLG
                     LQCNLGANSWTVFSDGIAHHSMLTIGQASQITGLVMIAIGWFVGVRPGLGTVLNMLLV
                     GWFLDLELQHGWIPQAESYPARLAMMLATVAVLGFATGLYIKAGFGAGPRDSFMLAVT
                     RLTGWPVGLNRWLIESGAVLAGALLGGAFGVGTILMALMIGPAVGVGFRLFGLPTRKR
                     GERRKEPIG"
     gene            complement(1454..2029)
                     /locus_tag="VFU72_05450"
     CDS             complement(1454..2029)
                     /locus_tag="VFU72_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5422965.1"
                     /translation="MLTLLHILIVVSFIVWVAELYWIYQQARLGGEDALSAVSGAAIF
                     WVLALPVAQRAPDLTDRLAAFWDRLAHLDFGGQREEPVPRLMVQGRAVPLTEERVRIG
                     RYPNNDIIIDHPTVSAYHAEVSLRPDGRHELVDRESRNGTRINGNPVRSAILRDGDQI
                     TLGAITLHYLVRAPSERTIQSGLSLPKRRLG"
     gene            2149..2466
                     /locus_tag="VFU72_05455"
     CDS             2149..2466
                     /locus_tag="VFU72_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422966.1"
                     /translation="MDDLEQVEILAEVLRERLPTVTHIIDEWSVAGSDAVSYAQIKDL
                     IWQIAAPLGLAMLGYSAAERARQDEPGNLEVGEAQALFARAGEDCGGVLGLFLGAASR
                     VRD"
     gene            complement(2463..>2985)
                     /locus_tag="VFU72_05460"
     CDS             complement(2463..>2985)
                     /locus_tag="VFU72_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422967.1"
                     /translation="FGRDRRYINGSAPTTADMREERERAWAERFPGPSSLEDYRRGFL
                     RYSPLFWDIVQSSQHDLLRLLTGRVPAELGVPAIFGLTVLFGRHPKADAATRAALGMI
                     VNELQPGQARTLLVSLADAWQNAERRPYDQRGPQVADEFERALRRLALSQAEDDGVIS
                     TLNTLVGADERED"
BASE COUNT          479 a         1026 c         1039 g          441 t
ORIGIN      
        1 cggcccgcaa gcaggggcac tcacccctgg tcgccctgga ggccgtcttc gccgggcggc
       61 ccctcgtcct cacctgacct gaatagttac gactgcgctc aggatgacgg gggaagggag
      121 gggcggacga atacgacgtg acgtactcgt acgccccatc ccctcccctt gtcagcctga
      181 gcttgtcgaa ggatccgtat cccaatggca cccaacgtcg tcgctggccg ccgctagctg
      241 tccgtcaccc ggatcatcag gggctgcttg ttccagtgcg acagcagctc ggccagcagg
      301 ttgcgcagct ggttgtcgct cagggcgttg ctgaccgggc gcacaccgct gaaggggagg
      361 ctggcgaact tctccttgct ggcgctggag cgcacgtcgc tgcgccagcc gacgatgttg
      421 agctgggcga tcacgcgctc gccgtcgttc atgaagtaga gcccgtactg cgggtcgagc
      481 gtcagcccga agtagcccgc cgccaggtta tccaccacta ccgggtagcc ggggtcgctg
      541 cgcgtcacgt actcgcgcgt gatcgcccgc agcatcggca gcatgcgctc gcgcagcgcc
      601 cgcagctcgg ccgtcacccc cgactccgca cccgcgtcga tactcaccct attggctcct
      661 tcctgcgttc gccacgcttc ctggtcggca ggccgaagag gcggaagccg acgcccaccg
      721 ccggtccgat catcagggcc atcagaatcg tacccacgcc gaacgcgccg cccagcagcg
      781 cgccggccag caccgcgccc gactcgatca gccagcgatt cagccccacc ggccagccgg
      841 tcagccgcgt caccgccagc atgaagctgt cgcgcgggcc ggcgccgaag ccggccttga
      901 tgtagagacc ggtggcgaag ccgagcacgg ccaccgtcgc cagcatcatc gccagccgcg
      961 ccgggtagga ctcggcctgg gggatccagc cgtgctgcag ctccagatcg aggaaccagc
     1021 cgaccagcag catgttgagc accgtgccca ggcccggccg caccccgacg aaccagccga
     1081 tggcgatcat caccagcccg gtgatctgcg aggcctgccc gatggtgagc atgctgtggt
     1141 gcgcgatgcc gtcgctgaag acggtccagg agttggcgcc gaggttgcac tgcaagccca
     1201 gcgtcagccc ggtggcgaag atgaacagac cggcgatcag catgacgaag cgccaggccc
     1261 cggtcagcca ttggtctccg ctccagcggg gcatgtcccg tagccgcagg gtgctgctgt
     1321 gatccaaggt cttcctcacg tcatacccgc cgccggtcgc gccccggcgg cgttgtgtct
     1381 cgcacaacgc acaacgcaca acgctcaacg caacgggcca cgcagaaatc gcaccggagg
     1441 ggaagcgctc ccgctagccc agccgccgct tcggcagtga caacccgctc tggatggtac
     1501 gctcgctggg cgcccgcacc agataatgga gcgtgatcgc gccgagcgtg atctgatcgc
     1561 cgtcgcgcag gatggccgag cggaccgggt tgccgttgat gcgggtgccg ttgcggctct
     1621 cgcggtcgac cagctcgtgc cgcccgtcgg ggcgcaggct gacctcggcg tgataggcgg
     1681 agacggtcgg gtggtcgatg atgatgtcgt tgttggggta gcggccgatg cgcacccgct
     1741 cctcggtcag cgggacggcg cggccctgca ccatcaggcg cggcaccggc tcctcgcgct
     1801 ggccgccgaa gtcgagatgt gccaggcggt cccagaacgc ggccaggcgg tcggtcaggt
     1861 cgggggcgcg ttgggcgacg gggagggcca gcacccagaa gatggccgcg cccgagacgg
     1921 cgctgagcgc atcctcccca ccaagccgcg cctgctggta gatccagtag agctcggcga
     1981 cccagacgat gaagctcacc acgatcagaa tgtggagcag cgtcagcatg gccccgtcct
     2041 ccctctccgc tctggtgatg cgagtatacg gcagtttggc acgactcgcg cggaacgtcg
     2101 acaatgagga aacgcgggcg acgtggcgta ggcacgggag gagcgaccat ggacgacctg
     2161 gagcaagtcg agattctggc ggaggtgctg cgggagcggc tgcccacggt gacgcatatc
     2221 atcgacgagt ggagcgtggc cggctccgac gcggtcagct acgcccagat caaggatttg
     2281 atctggcaga tcgccgcgcc gctgggcctg gcgatgctcg gctacagcgc cgccgagcgt
     2341 gcccggcagg acgaaccggg caacctggag gtcggcgagg cccaggccct cttcgcgcgg
     2401 gccggcgagg attgcggcgg cgtcttgggg ctctttctgg gcgcggccag ccgggtacga
     2461 gactagtcct cgcgctcatc ggctcccacg agcgtgttca gggtggagat gacgccgtcg
     2521 tcttcggcct gggacagcgc caggcggcgt agggcgcgct cgaactcgtc cgccacctgc
     2581 ggcccgcgct ggtcgtaggg ccgccgctcc gcgttctgcc aggcatcggc gaggctgacc
     2641 aagagcgtgc gtgcctgccc cggttgcagc tcgttgacga tcatgcccag cgcggcgcgc
     2701 gtggcggcgt cggccttggg gtggcggccg aagaggacgg tcaggccgaa gatcgccggc
     2761 acgcccagct cggccggcac gcgcccggtc agcaggcgca ggaggtcgtg ctggctcgac
     2821 tgcacgatgt cccagaagag gggcgagtag cgcaggaagc cgcggcgata gtcttccagg
     2881 ctggacggac cggggaagcg ctcggcccag gcccgctcgc gctcctcccg catgtcggcg
     2941 gtcgtcggag cggatccgtt gatgtaccgg cgatcccgtc caaaa
//
LOCUS       DASUBI010000287         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1140056, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000287 DASUBI010000000
VERSION     DASUBI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140056"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1013
                     /locus_tag="VFU72_05465"
     CDS             105..1013
                     /locus_tag="VFU72_05465"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR01505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEU5422968.1"
                     /translation="MAESLGFIGLGIMGKPMALNLLKAGYKLVVHSRSRGPVDELVQA
                     GATAAASPSAVAGQVKTVITMLPDSPDVRLVALGPDGLIDSMGQGSLLIDMSTISPLV
                     TQEVAGALAEKGAGMVDAPVSGGDKGAIAASLSIMAGGSEADFARALPIFQALGKTIV
                     HVGDIGAGQIVKACNQVVVAINFEALSEALVLGSKAGVDPEKILQVLGGGLAGSKVLE
                     LRGQSMLAHNFQPGFRLRLHRKDLNIALSTARSLDVPLPVTALVDQFITALVAAGRGN
                     LDHSAILTELEDLAHYTVGSGEQREE"
     gene            1349..2287
                     /gene="hpaI"
                     /locus_tag="VFU72_05470"
     CDS             1349..2287
                     /gene="hpaI"
                     /locus_tag="VFU72_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HEU5422969.1"
                     /translation="MASRNSRKELKGSIVPLVTPFKHGQFDEAAFRELIEWQIESGSH
                     GISMTGSTGEPAALTPEEREHVIATTVSAVRGRVPVVAGTGTNNFDETVRYTRFAERA
                     GADAALVMVPYYNRPSQEGLYQHFSGVADATSLPVIIYNIPGRTAVNMEPETMARVMR
                     DHKNVVGVKEANKDFEQVTKVFDRCGRDTLVYSGIEALCFPLLALGGSGYISATGNVL
                     PRQTAELYNLVAAGKWKEARDLHYESFPMNEALFWETNPGPVKYVLSLMGKIAPELRL
                     PLVLPSEANQRKLRQVADKYGLVPAAAVAGGEDADR"
     gene            2284..3033
                     /locus_tag="VFU72_05475"
     CDS             2284..3033
                     /locus_tag="VFU72_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5422970.1"
                     /translation="MRTVRFMAQGRLHTGQPHPDGDRLIDEAGHSHRQNEVTWLPPVV
                     PSKVLGLVLNYAEHAKELGLETPQAPVLFFKPLTSLIGHHEPIVYPDGVEQLHYEVEL
                     GVVIGRRCRNVRASDAFSVVRGYTIGNDVTARDFITNYFRPPVKAKGFDTFGPLGPWV
                     VEDDNIDPDNLELRAFVNGELRQQGNTSKLIHKVPEIIEYITGFMTLEPDDVILTGTP
                     EGISYVRPGDTLRVEVEGVGALENPIIPQPS"
     gene            3081..>4250
                     /gene="hpaB"
                     /locus_tag="VFU72_05480"
     CDS             3081..>4250
                     /gene="hpaB"
                     /locus_tag="VFU72_05480"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HEU5422971.1"
                     /translation="MGARTGEQYLAGLRDHPREVWIHGERVQGDITKHPAFRNVTRSV
                     ASLYDMQCDPELQSEMTYVSPSSGERVGLSFLQPRSRDDILRRRVMMKHWADYSGGMM
                     GRSPDYLNSGLMAMAAAAEFFAQNDPRHAPNMRQYYEHAREHDLCLTHTLITPQVNRS
                     LGPSQQSDPFIAARVVKETDDGLIVRGARLLATLGPIADEIEVFPSTVLRGGPEDKPY
                     TFAFSIPCDTPGLKFICRESFDYGKTPFDHPLGARFEEMDAVVVFDDVLVPWERVFLY
                     GDVELANGVFSETNAVIHMAYQVIVKNQAKAEFILGIAASIADRIGIDQFQHVQEKLS
                     EIIVAVEMMRAFLRASEADAAVDRWGLMTPAWGPINAARNLFPKLYPRLVEIIQQL"
BASE COUNT          737 a         1456 c         1395 g          662 t
ORIGIN      
        1 tactgggaga agggggagta cgtaacagga gcacttcgta ctcccctctc ccagtattgg
       61 gagaggggcc gggggtgagg gcccgtccga gaagggagtc gatcgtggcc gagagtctgg
      121 ggttcatcgg gctggggatc atgggcaagc cgatggcgct caatctcctc aaagccggct
      181 acaagctggt cgtgcatagc cgtagccggg gaccggtcga cgagctagtt caggcgggcg
      241 ccacggcggc cgcaagcccg agcgccgtcg ccgggcaggt caagacggtg atcaccatgc
      301 tgcccgactc gccggacgtg cgcctggtgg cgctcggccc cgacggcctg atcgattcca
      361 tggggcaggg cagcctcttg atcgacatga gcaccatctc gccgctggtg acgcaggagg
      421 tcgccggggc gctggccgag aagggcgccg ggatggtcga cgcgcccgtc agcggcggcg
      481 acaagggcgc catcgccgcc tcactcagca tcatggccgg cggtagcgag gcggacttcg
      541 cgcgggcgtt gccgatcttc caggcgctcg gcaagacgat cgtgcacgtg ggggacatcg
      601 gcgccggcca gatcgtcaag gcctgcaacc aggtggtggt ggcgatcaac ttcgaggcgc
      661 tcagcgaagc actggtactc ggcagcaaag ccggcgtcga ccccgagaag atcctgcagg
      721 tgctcggcgg cggactggcc ggcagcaagg tgctcgagct gcgcggccag tcgatgctgg
      781 cccacaactt ccagcccggc tttcgcctgc gcctgcaccg caaggacctc aatatcgccc
      841 tctccaccgc gcgtagcctc gacgtacccc tgccggtgac cgcgctcgtc gatcagttca
      901 tcaccgcgct ggtcgccgcc ggccgcggca acctcgacca ctccgccatc ctgaccgagc
      961 tggaggatct ggcgcactac acggtgggtt caggggagca gcgggaggag tagagggcgc
     1021 ggtactggaa gctgtcgagc aggcattggt gccgggtgag gacggatcct tcgacttcgc
     1081 tcaggatgac agggttaatt tggggctgac gaggatggcg tgcctcgtcc gataggcctc
     1141 atctcctacc gtgtcatcct gagcgcagtc gaaggatcta tcttgtccga catcccatcc
     1201 acgtcgtcag ctacctcccc tctcccgcgc accggaagag gggccggggg tgcggggccc
     1261 cgtctgttgg cgtccacggc gcaaggtgcg atagtgttgc tacgtttccg gccatcgggc
     1321 atggcacacg ctggagtgga ggacgctgat ggcttcgcgc aattcgcgca aggaactcaa
     1381 aggctcgatc gttcccctgg tcacgccgtt caagcacggg cagttcgacg aggccgcctt
     1441 tcgcgagctg atcgagtggc agatcgagag cggcagccac ggtatctcga tgaccggctc
     1501 gaccggcgag ccggccgcgc tgactcccga agagcgcgag cacgtcatcg ccaccacggt
     1561 cagcgccgtg cgcgggcgcg tgcccgtggt cgccggcacc ggcaccaaca acttcgacga
     1621 gactgtgcgc tacactcgct tcgccgagcg cgccggcgcc gacgccgcgc tggtgatggt
     1681 cccctactac aaccggccca gccaggaggg cctctatcag cacttcagcg gcgtggccga
     1741 tgcgacctcg ctgccggtca tcatctacaa catccccggc cgcaccgccg tcaacatgga
     1801 gccggagacg atggcgcgcg tgatgcgcga ccataagaac gtcgtggggg tcaaagaggc
     1861 caacaaggat ttcgagcagg tcaccaaggt cttcgaccgc tgcgggcgcg acacgctggt
     1921 ctactccggc atcgaggcgc tctgcttccc gctgctggcg ctcggcggct ccggctacat
     1981 cagcgccacc ggcaacgtgc tgccgcgcca gacggccgag ctctacaacc tggtggccgc
     2041 gggtaaatgg aaggaagcgc gcgacctcca ctacgagtcc ttcccgatga acgaggccct
     2101 cttctgggag accaaccccg gcccggtcaa gtacgtgctg agcctgatgg gcaagatcgc
     2161 gccggagctg cgcctgccgc tggtcctccc ctcggaggcc aaccagcgca agctgcgcca
     2221 ggtcgcggac aaatacggct tggtgccggc cgcagcggtg gccggcggag aggatgcgga
     2281 tcgatgagga ccgtgcgctt catggcccag ggacgtctgc acaccggcca gccccaccct
     2341 gacggggatc gcctgatcga cgaggccggg cacagccacc gccagaacga agtgacgtgg
     2401 ctgccgccgg tggtgccgag caaggtgctc ggcctggtgc tgaactacgc cgagcacgcc
     2461 aaggagctgg gcctggagac accgcaggcg ccggtcctct tcttcaagcc gctgacttcc
     2521 ctgatcggcc accacgagcc gatcgtctac ccggacggcg tcgagcagct gcactacgag
     2581 gtcgagctgg gcgtggtcat cggccgccgc tgccgcaacg tgcgcgcctc tgacgccttc
     2641 tcggtcgtgc gcggctacac catcggcaac gacgtcaccg cgcgcgactt catcaccaac
     2701 tacttccgcc cgccggtcaa ggccaaaggc ttcgatacct tcggcccgct tgggccctgg
     2761 gtggtcgagg atgacaacat cgacccggat aatctcgagc tgcgcgcctt cgtcaacggc
     2821 gaattgcgcc agcagggcaa cacctccaag ctgatccaca aggtgcccga gatcatcgaa
     2881 tacatcactg gcttcatgac gctggagccg gacgatgtga tcctgaccgg cacgcccgaa
     2941 ggcatctcct acgtgcgtcc cggcgacacg ctgcgggtcg aggtggaggg cgtcggcgcg
     3001 ctggagaacc cgatcatccc ccagccgtcc tgagccggcg ggcggcggag cagggcgaaa
     3061 gcgaaacgga ggaggcgacc atgggtgccc ggacgggcga gcaatacctg gcggggctgc
     3121 gcgaccatcc gcgcgaggtc tggatccacg gcgagcgggt gcagggcgac atcaccaagc
     3181 accccgcctt ccgcaacgtc acgcgcagcg tggcctcgct ctacgatatg cagtgtgatc
     3241 ccgagctaca gagcgagatg acctacgtct cgccaagcag cggcgagcgc gtcggcctct
     3301 ccttcctcca gccgcgctcg cgcgacgaca tcctccgccg ccgcgtgatg atgaagcact
     3361 gggccgacta ctccggcggc atgatgggcc gctcgcccga ctacctcaac agcggcctca
     3421 tggcgatggc cgccgccgcc gagttcttcg cccagaacga tccgcgccac gcccccaaca
     3481 tgcgccagta ctacgagcac gcgcgcgagc acgatctctg cctgacccac acgctgatca
     3541 cgccgcaggt caaccgctcg ctcggcccca gccagcagtc cgaccccttc atcgcggcgc
     3601 gcgtggtcaa ggagaccgac gacgggttga tcgtgcgtgg cgcgcgcctc ttggccactc
     3661 tcggcccgat cgccgacgag atcgaggtct tccccagcac cgtgctgcgc ggcggtccgg
     3721 aggacaaacc ctacaccttc gccttctcga tcccctgcga cacgccgggg ctgaagttca
     3781 tctgccgcga gtcattcgac tatggcaaga cgcccttcga ccacccgctc ggcgcccgct
     3841 tcgaggagat ggacgcggtg gtggttttcg acgacgtgct ggtcccctgg gagcgcgtct
     3901 tcctctacgg cgatgtcgag ctggccaacg gggtcttcag tgagaccaac gcggtcatcc
     3961 acatggccta ccaggtgatc gtcaagaacc aggcgaaggc cgagtttatc ctgggcatcg
     4021 ccgcctcgat cgccgatcgc atcggcatcg accagttcca gcacgtgcag gagaagctct
     4081 ccgagatcat cgtcgcagtc gagatgatgc gcgccttcct gcgcgccagc gaggccgacg
     4141 ccgccgtcga ccgctggggt ctgatgacgc ccgcctgggg gccgatcaac gcggcgcgca
     4201 acctcttccc gaagctctat ccccggctgg tcgagatcat ccagcagctc
//
LOCUS       DASUBI010000288         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_97923, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000288 DASUBI010000000
VERSION     DASUBI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97923"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFU72_05485"
     CDS             complement(<1..1176)
                     /locus_tag="VFU72_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422972.1"
                     /translation="MTGPDPSLGQLLRRYRLLAGLTQEELAERSAYSTDYISKLERDQ
                     RQPPLVTIDRLAAVLGLGQPEHQTLRAARERHERAAPASRAPRWAPLPAAPTPLVGRV
                     AEQAAVWSALDTSRLVTLTGAGGTGKTRLALEVARRAAEGRAFADGVAFVDCGPLTSH
                     GLLGPTIARALGLVEQPDLPSELRLLEALRSRHLLLVIDTLEHLLAETPLLSRLLAAA
                     PQLHILATSRAPLHLYGEYEVHVPPLSLPSAGGEPSDQGADASEAVQLFVARARAVAP
                     GFDAGGSARATLGALCRALDGLPLAIELAAARVTLFPPEALLDRLGECLAVLADGPRD
                     LPHRQQTLRATLDWSFRLLAPPEQALFTHLGVFAGSFDARAAAAICTPDTASGPEPEL
                     "
     gene            1818..>2200
                     /locus_tag="VFU72_05490"
     CDS             1818..>2200
                     /locus_tag="VFU72_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422973.1"
                     /translation="MKITLRFLLAALLVLTLVGLGSSALAAPSGNADAAHLCQDGGYA
                     SLQGSDGTTFSNAGECVSYAAQGGTIVGINACVVTSTTGCLTLDNVTLQSGSANGDTM
                     TVTGSISFKTACPYTPPANGICYMSG"
BASE COUNT          345 a          755 c          793 g          307 t
ORIGIN      
        1 aagctccggc tctggcccag acgcggtatc tggggtgcag atcgcggcgg cggccctggc
       61 atcgaaggag ccggcgaaga ccccaagatg ggtgaagagc gcttgctcgg gcggcgcaag
      121 caggcgaaag ctccaatcga gggtggcgcg gagtgtttgc tgccggtgcg gcagatcgcg
      181 cggcccgtcg gcgaggacgg ccagacactc gccgagccgg tcgagcaacg cctccggcgg
      241 aaagagcgtg acccgagcgg cggccagctc gatggccaga ggaaggccat cgagggcgcg
      301 gcagagcgcg cccagcgtcg cccgcgcgct gccgccggcg tcgaaaccag gggcgacggc
      361 ccgcgctcgc gccacgaaga gctgcacggc ctcgctggcg tccgctccct gatcgctggg
      421 ctcgcctccg gccgacggca acgacagggg tgggacatga acctcgtatt caccatagag
      481 gtggaggggt gcccggctgg tggccaagat gtgcagctgg ggcgcggcgg ccagcagccg
      541 gctcaagagc ggtgtctccg ccagcaggtg ctccagcgta tcgatcacca ggagcagatg
      601 gcgcgaccgg agcgcttcga ggagtcgcag ctcagacggc aggtcgggct gctcgaccag
      661 ccccagcgcc cgggcaatgg tcggacctag cagaccgtgg ctggtcaacg gcccgcaatc
      721 gacgaaggcg accccgtcgg cgaaggcgcg gccttccgcg gcgcgccgcg ccacctccag
      781 cgccagccgg gtcttgccgg ttccgccagc gccggtgagg gttaccaggc gagaggtgtc
      841 gagcgcgctc cagacggccg cctgttcggc cacccgcccg acgagcggtg tcggtgccgc
      901 cgggagcggg gcccaccgcg gcgcacggct cgccggcgcg gcgcgctcgt gtcgctcccg
      961 ggcggcgcgc agcgtctggt gctccggctg accgaggccc agcacggcag ccaaccgatc
     1021 gatggtcacc aggggcggct ggcgctgatc gcgctccagc ttgctgatgt agtcggtgct
     1081 gtaggcgctg cgctcggcca actcctcctg cgtgaggccg gccagcaggc ggtagcgccg
     1141 gagcaactgc cccaaggacg gatcaggccc ggtcacctat acccttagcc ttgcctccag
     1201 agcgcgacgc gcgccggccc ggctcgtgca atcaatgcct gtacgcacac tatgtctagc
     1261 tttctcgctt gtcaaccgcc gaccacacga ttttgtccgg gtgtttgtcc gggtttgtct
     1321 gtggtcaatg aggcgagtga cggccagact gggagtgtgt tcgatgccgc tcgatggcgc
     1381 gacggcgcgg cggtcggcgg cgccgggagg gctcttcggt gggcagcagg atggcgatga
     1441 cgatggagct gcccgcgccc gcgacgtcac gtcgtgtcgg aggcgggcag ccgagccgcg
     1501 ggacgccccg actcagcgcg caggccgcgg ctgctccaca ggagcggtcg ccgttctcgt
     1561 ccgccacgct caccgccggc ggccctgcgc accgtgtcgc tgctggggtc actgggggcg
     1621 cggccacgag catggcgaac cgcacccgac gcgaccgcgc ccagttacac cgcgcctggg
     1681 cggttgaaga gaggaggaat cgggagatcg gcgacctgta cgacacctga ctaatcggtc
     1741 tgatcagaga ggagcgcgcg atgaccacgc atcgagcggc tgagcgccgc caccgcccac
     1801 gccaaggaga gcattccatg aagatcaccc tgcgttttct gctcgccgcg ctgctggtgc
     1861 tgacgctggt cggcctgggg agcagcgccc tcgccgcgcc gagcggcaac gccgacgccg
     1921 cccatctgtg ccaggacggc ggctacgcca gcctgcaggg gagcgacggc acgaccttca
     1981 gcaacgcggg cgagtgtgtc agctacgcgg ctcagggcgg gaccatcgtg ggcatcaacg
     2041 cctgcgtggt caccagcacc accggctgcc tgaccttaga caatgtcacc ctccagtcgg
     2101 gttcggccaa cggcgacacc atgaccgtca ccggctcgat ctccttcaag acggcctgtc
     2161 cgtatacgcc gcccgccaat ggaatttgtt acatgtcggg
//
LOCUS       DASUBI010000289         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_937720, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000289 DASUBI010000000
VERSION     DASUBI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937720"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..3372)
                     /locus_tag="VFU72_05495"
     CDS             complement(175..3372)
                     /locus_tag="VFU72_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5422974.1"
                     /translation="MSVQSQIDPALLKALKWRLIGPFRGGRVVAVAGDPADPFTFYFG
                     ACDGGVWKTSDGGNVWENVSDGFFNTAPVGALAVAPSDPNVIYAGMGETAIRGNVSEG
                     DGVYKSTDGGQTWQHMGLADTRAIGRIRVHPNNPDLVYVAALGHIFGQNEERGVFRSQ
                     DGGQSWQKVLYKSPKAGAVDLSMDASNPRILYASLWEAQRHPHTLISGGPDSGLYKSS
                     DGGDTWQEISHNKGLPQGVLGKIGVAASPARAGRVWALVEAEDGGLFRSDDGGNSWQR
                     LNDEPDLRQRPWYYMHIYADPQEADTVYVLNLRVWKSVDGGKSFSGIPTAHGDNHDLW
                     IDPNNTKRMVEGNDGGACVSFNGGRSWSTLYNQPTAQFYHVVTDSRTPYFVYGAQQDN
                     STLAGPSRSDAGSITMGEWYPVGGGESGYIAVRPDDPDIVYAGSYGGLITRYDHRTRQ
                     QRNIAAWPENPMGYSAGELKYRFQWTYPIILSPHDPNVLYVCSQYVHRTSDEGTSWET
                     ISPDLTRAEQSKMGPSGGPITRDNTSVEYYGTIFSFAESPVQRGVLWAGADDGLIHVS
                     KDNGQSWQNVTPAANVLPEWALISLIEPSPHDAGTAYVAATRYKLDDLRPYLLKTSDF
                     GQNWQAITGGLPEDDFMRAIREDPQRQGLLYAGGEKGLYVSLDDGLHWQPFRQNLPLV
                     PIHDLAVADGDLIAATHGRSFWILDDLAPLRQLQPGVAGEAAHLFAPRATVRYRGGGR
                     RGGGGESQSERDFQRSGGVSVLTGRTPGGETVLLDAGQNPPTGVVVTYYLKEAPQGEV
                     TLAFLDAQGKELRAFSSEAKDEKGQGKSEGGGGQRRQREPRVPKDAGSNRFVWNLRLA
                     NATEVPGAIIWAGNLQGPQVPPGKYQVRLTAGGQSYTQPFEVVKDPRLPTSQQDFEEQ
                     YQLLLKIGDKLSQTHAAINEIRELRQQAESWQTRAERAEQGKPVAEAAKELTEKLTAI
                     EEELIQVKSKAMEDPLNYPIKLNNKLAALAGIVDSADAAPTKQSYEVFDHLARGVDTL
                     LDQLSALRENQVQRFNALVHQTQLPALSTQKAAAD"
     gene            3541..>4758
                     /locus_tag="VFU72_05500"
     CDS             3541..>4758
                     /locus_tag="VFU72_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5422975.1"
                     /translation="MPDLETALALLAVVLLVAALFSGLVARLPLSFPMIFLGLGFLLG
                     ERGLGVIHIGVDDPALEAIAIVSLSFVLFLDAINLRFDELGPGWRVPLLALGPGTLLT
                     VALVALGAALALGLSPLNALLLGAILASVDPVVLRDVARDRRIPRSIRLALTTEAGTN
                     DVIVLPLVLVLSAIEIGGVSGVTAWLALLARLFLLGPLVGAALGFALVWLIAKARAHE
                     LISREYRALYGVGGVLAAYVAGEAVGGSGFLAVFAAGVVVAATDYDLCDCFLEFDEVT
                     AEMAMLLAFILFGALLSTLLGSVPPARTLLFALLVLAVARPLALGVVLARAVVSRPAR
                     LFMGWFGPRGLSSLLFGLLVVRRGVPDGERLLALSGAVVVVSVVLHGVSEVPLAGWYG
                     RAVERQTLPEEREG"
BASE COUNT          549 a         1641 c         1698 g          870 t
ORIGIN      
        1 gccactaccc ctgtgggggc agacctccgt gtccgccctc gttggatggg cacccagcct
       61 tctcgaacgc cacccacgtt caccccttcc ctgtcgccga ggcgtaaggg aacgagggca
      121 gacacggagg tctgccccta cagcgtacgt ttcgcagccg tactccggaa gccctcagtc
      181 agccgccgcc ttctgcgtgc tgagcgccgg tagctgggtc tggtgcacca gcgcgttgaa
      241 gcgctgcacc tggttctcgc gcagcgccga gagctggtcg agcagcgtgt cgacgccgcg
      301 cgccaggtgg tcgaagacct catacgactg cttcgtcggg gccgcgtcgg cgctgtcgac
      361 gatgccggcc agcgcggcca gcttgttgtt cagcttgatc gggtagttga gcgggtcctc
      421 catcgccttc gatttgacct ggatcagctc ctcctcgatc gcggtcagct tctcggtcag
      481 ctccttggcg gcctcggcta ccggcttgcc ctgctccgcg cgctcggctc gcgtctgcca
      541 gctctccgcc tgctggcgta gctcgcggat ctcgttgatc gccgcgtgtg tctgagagag
      601 cttgtcgccg atcttcagca ggagctggta ctgctcctcg aagtcctgct gcgaggtcgg
      661 caggcgcggg tccttgacca cctcgaacgg ctgggtgtag ctctggccgc cggctgtcag
      721 ccgtacctgg tacttgcccg gcggcacctg tggcccctgg agattgccgg cccagatgat
      781 cgcgcccggc acctcggtcg cgttggccag gcgcagattc cagacgaagc ggttgctgcc
      841 agcgtccttg ggcacgcgcg gctcgcgctg gcggcgttgg ccgcctccgc cctcactctt
      901 gccctggccc ttctcgtcct tggcctcgct ggagaaggcg cgcagctcct tcccttgggc
      961 gtcgaggaag gcgagcgtca cctcaccctg cggtgcctcc ttgaggtagt aggtcacgac
     1021 cacgccggtg ggcgggttct ggccggcgtc gagcaggacc gtctcgccgc ccggcgtgcg
     1081 cccggttagc acgctgacgc cgcccgagcg ctggaagtcg cgctcgctct ggctctcgcc
     1141 gccgcccccg cgccggccgc ctcctcgata gcggacggtg gcgcgcggcg cgaagaggtg
     1201 cgccgcctcg ccggccacgc ccggctgcag ttggcgcagc ggcgcgaggt cgtcgaggat
     1261 ccagaaggag cggccgtggg tggcggcgat caggtcgccg tcggccaccg ccaggtcgtg
     1321 gatcggcacc agcggcaggt tctgccggaa cggctgccag tgcaggccgt cgtcgagcga
     1381 gacgtagagc cccttctcgc cgccggcgta gagcagcccc tggcgctgcg ggtcctcgcg
     1441 gatggcgcgc atgaagtcgt cctcgggcag cccgccggtg atcgcctgcc agttctggcc
     1501 gaagtcgctg gtcttgagga ggtatggccg caggtcgtcg agcttgtagc gggtcgcggc
     1561 gacataggcc gtgccggcgt cgtgcggcga gggctcgatc aacgagatca gcgcccactc
     1621 gggcaggaca ttggccgcag gcgtgacgtt ctgccagctc tggccgttgt ccttcgagac
     1681 gtggatcagg ccgtcgtcgg cgccggccca gagcacgccc cgctgcaccg gcgactcggc
     1741 aaagctgaag atggtgccgt agtactcgac gctggtgttg tcgcgcgtga tcggcccgcc
     1801 cgatggcccc atcttgctct gctcggcgcg cgtcaggtcg ggcgagatgg tctcccagct
     1861 cgtgccttcg tcgctcgtgc ggtgcacgta ctgcgagcag acgtagagca cgttggggtc
     1921 gtgcggcgag aggatgatgg ggtaggtcca ctggaagcga tacttgagct cgccggccga
     1981 gtagcccatc gggttctccg gccaggcggc gatgttgcgc tgctggcggg tgcggtggtc
     2041 gtagcgggtg atcaggccgc cgtagctgcc ggcgtagacg atgtcggggt cgtcggggcg
     2101 cacggcgatg tagccgctct cgccgccgcc caccgggtac cactcgccca tcgtgatcga
     2161 gccggcgtcg gagcggctcg gtccggccag ggtgctgttg tcctgctggg cgccgtagac
     2221 gaagtagggc gtccggctgt cggtgaccac atgatagaac tgcgcggtcg gctggttgta
     2281 gagcgtcgac cagctgcgcc cgccgttgaa gctgacgcag gcgccgccgt cgttcccctc
     2341 gaccatacgc ttggtgttgt tggggtcgat ccagaggtcg tggttgtcgc catgggcggt
     2401 cgggatgccg ctgaagctct tgccgccgtc gaccgacttc cagacgcgca ggttgagcac
     2461 gtagaccgtg tcggcctcct gggggtcggc gtagatgtgc atgtagtacc aggggcgctg
     2521 gcgcaggtcg ggctcgtcgt tgagccgctg ccaggaattc ccgccgtcgt cggagcggaa
     2581 gaggccgccg tcctcggcct cgaccagcgc ccagacgcgc cccgcgcgcg cgggtgaggc
     2641 ggccacgccg atcttgccca gcacgccctg cggcagcccc ttgttgtgcg agatctcctg
     2701 ccacgtgtcg ccgccgtcgc tcgacttgta gaggccgctg tcgggcccgc cgctgatcag
     2761 cgtgtgcggg tggcgctgcg cctcccagag cgaggcgtag aggatgcgcg ggttgctggc
     2821 gtccatactg aggtcgaccg cgcccgcctt cgggctcttg tacagcacct tctgccagct
     2881 ctggccgccg tcttgggaac gaaagacgcc gcgctcctcg ttctgcccga agatgtggcc
     2941 cagcgcggcc acgtagacca gatcagggtt attcgggtgg acccggatgc ggccgatggc
     3001 gcgcgtgtcg gccagcccca tatgctgcca ggtctgcccg ccgtcggtcg acttgtagac
     3061 gccgtcgccc tcggagacgt tgccacggat ggcggtctca cccataccgg cgtagatcac
     3121 gttggggtcg gagggcgcga cggcgagcgc gccgaccggc gcggtgttga agaagccgtc
     3181 ggagacgttc tcccagacat tgccgccgtc gctcgtcttc cagacgccgc cgtcgcaggc
     3241 gccgaagtag aaggtgaagg ggtcggccgg gtcgccggcg acggccacca cgcgcccgcc
     3301 gcggaacggg ccgatcaagc gccacttgag cgccttcaac aacgcgggat cgatctgact
     3361 ctgcaccgac atggtcttcc tccctgtcgc actgcggccc ggcggacgcg accactccca
     3421 ctctcgcccg gcccgccttc agtcggttcc cgattgtcac gccgggtggg aggcgtgtca
     3481 aaccgccggg gagcggatcg gcgcggtgcg ctgctatcct tcccgctgga aggaggcgcc
     3541 gtgcccgatc tggagaccgc gctggcgttg ctggccgtag tgctgctcgt cgcggcgctc
     3601 ttctccggcc tggtggcgcg cctgccgctc tcgtttccca tgatcttcct cgggctcggc
     3661 ttcctgctgg gcgagcgcgg gctgggtgtc atccacatcg gcgtcgacga tccggcgctc
     3721 gaagccatcg ccatcgtcag cctctccttc gtcctctttc tggatgccat caacctgcgc
     3781 ttcgacgagc tgggcccggg ctggcgtgtg ccgctgctgg cgcttggccc cggcacgctc
     3841 ctcacggtgg cgctggtcgc cctcggcgcc gcgcttgccc tcggcctctc gccgctcaac
     3901 gcgctgctgc tgggcgcgat cctggcctcg gtcgatcccg tggtgctgcg cgacgtggcg
     3961 cgcgaccggc gcatcccgcg ttcgatccgg ctggcgctga ccaccgaggc gggcaccaac
     4021 gatgtcatcg tgctgccgct cgtgctggtg ctgagcgcga tcgagatcgg cggggtcagc
     4081 ggcgtgacgg cctggctcgc gctgctggcg cggctcttcc tgctcgggcc actggtcggc
     4141 gcggcgctcg ggtttgcgct ggtctggttg atcgccaaag cgcgtgccca cgagctgatc
     4201 agccgcgagt accgcgccct ctatggcgtc ggcggcgtgc tggcggccta cgtcgccggg
     4261 gaggcggtcg gcggcagcgg cttcctggcg gtcttcgcgg ctggcgtggt cgtggcagcc
     4321 accgactacg acctctgcga ctgcttcctg gaattcgacg aggtcaccgc cgagatggcg
     4381 atgctgctgg ccttcatcct cttcggcgcg ctgctctcga ccctgctcgg aagcgtgccc
     4441 ccggcgcgta cgctcctctt cgccctgctg gtgctggccg tggcgcgccc gctggcgctc
     4501 ggcgtggtgc tggcgcgcgc cgtggtcagc cggcccgcgc gcctcttcat gggctggttc
     4561 ggcccgcgcg gcctcagctc gctcctcttc ggcctgctgg tcgtccgccg gggcgtgccg
     4621 gacggtgagc ggctgctggc gctctccggc gcggtcgtgg tcgtctcggt cgtgctgcac
     4681 ggcgtcagcg aggtgccgct ggccggctgg tatgggcggg ccgtcgagcg gcagaccctg
     4741 ccggaggagc gtgagggc
//
LOCUS       DASUBI010000290         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_285258, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000290 DASUBI010000000
VERSION     DASUBI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285258"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFU72_05505"
     CDS             <1..269
                     /locus_tag="VFU72_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422976.1"
                     /translation="GLFVAHNITNNTQVDVVARGVVRALREAGLDARDFPVVAREIGV
                     NDAEGAAIFAAAGVRYLGAGSTLSDAARAMVRAMRERVASETGG"
     gene            266..1207
                     /locus_tag="VFU72_05510"
     CDS             266..1207
                     /locus_tag="VFU72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5422977.1"
                     /translation="MMLVDETTRVVIQGITGREARMVTAHMLAYGTQVVAGATPGRGG
                     SEVDGVPVYDTLEEVMARHAPTASLISVPPGAVLDACLEALDAGLRLLVIATEGMPQH
                     DAMLALEAARAARARIIGPNSVGVINPAARVKLGAIGGDNPARAFPPGGIGIISRSGG
                     MTAEIGLMLKRLDLGVSTAVSVGGDALIGTPPARLLPLYQRDAHTEGVVLFGEPGTRF
                     EEEVAETIQARRYTKPLVAMVAGRFTESLPEGTAFGHAAAIIQGAGGRPSRKLERLRE
                     AGALVAEDIDDLLQYLSLVFKNAPYLGRSLDGPRLDR"
     gene            complement(1545..2771)
                     /locus_tag="VFU72_05515"
     CDS             complement(1545..2771)
                     /locus_tag="VFU72_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5422978.1"
                     /translation="MANIAIIAVALIAGAAVGTYFARTVAMTAMPQMVALFNGMGGGT
                     AALVSVAEFVRFYNDSGSVGGGESLSVVVGTTIGAISFAGSMIAFGKLQELISGRPIR
                     FAGGQIVNAVILIAILALWVIILLGSNTLPVWGLLAAALILGVLVVLPIGGADMPVVI
                     AVLNSMTGLAAAAAGFVLDNQILIVAGALVGASGALLTVLMSRAMNRSLANVMFGAFG
                     SAPAGGAAATGEARPIRQTTAEDVAVQMAYANEVIIVPGYGLAVAQAQHQIRELADEL
                     EKRGVTVKYAIHPVAGRMPGHMNVLLAEANVPYDQLFEMEQINDEFERADVALVVGAN
                     DVVNPAARHDKGSPIFGMPILNVDQARSVIVLKRSMRPGFAGIENELFHEDKTAMLFG
                     DAKASLAALTNEVKAA"
BASE COUNT          407 a          975 c         1034 g          387 t
ORIGIN      
        1 gcgggctctt cgtggcccac aacatcacca acaacaccca ggtcgacgtg gtggcgcgcg
       61 gggtggtgcg ggcgctgcgg gaggccgggc tcgacgcgcg cgacttcccg gtggtggcgc
      121 gcgagatcgg cgtcaacgac gccgagggag cggccatctt tgcggcggcg ggcgtgcgct
      181 acctgggcgc cggcagcacg ctgagcgacg cggcgcgcgc gatggtgcgg gcgatgcgcg
      241 agcgcgtggc gagtgaaacg gggggatgat gctggtcgac gagacgacgc gcgtggtgat
      301 ccaggggatc accgggcgcg aggcgcgcat ggtgacggcg cacatgctcg cctacggcac
      361 ccaggtggtg gcgggggcca cgccggggcg cggcggcagc gaggtcgacg gcgtgccggt
      421 ctacgacacc ctggaggagg tgatggcacg gcacgcgccg accgcctcgc tgatctcggt
      481 gccacccggc gcggtgctcg acgcctgcct ggaggcgctc gacgcggggc tgcggctgct
      541 ggtgatcgcc accgagggga tgccccagca cgacgccatg ctggcgctgg aggcggcgcg
      601 cgcggcgcgg gcgcgcatca tcggccccaa ctcggtcggg gtgatcaacc cggcggcgcg
      661 cgtcaagctc ggcgcgatcg gcggcgacaa ccccgcgcgg gcctttccgc cgggcggcat
      721 cggcatcatc tcgcgcagcg gcggcatgac cgccgagatc gggctcatgc tcaagcggct
      781 cgatctgggg gtcagcacgg cggtcagcgt cggcggcgac gcgctcatcg gcacgccgcc
      841 cgccaggctc ctgccgctct accagcgcga cgcgcacacc gagggcgtgg tcctcttcgg
      901 cgagcccggc acccgcttcg aggaggaggt cgcggagacg atccaggccc ggcgctacac
      961 caagccgctg gtggcgatgg tggccggccg cttcaccgag tcgctgccgg agggaactgc
     1021 tttcggccac gccgccgcca tcatccaggg cgccggcggc cgcccgagcc gcaagctgga
     1081 gcggctgcgc gaggcgggcg cgctggtggc cgaggacatc gacgacctgc tgcagtacct
     1141 cagcctggtc ttcaagaacg ccccctacct gggcaggtcc ctcgatggcc cgcgcctcga
     1201 ccggtaggag cagacctccg tgtctgcccg gttgccctca cgcggcggca acggaggcgt
     1261 gggcagcgtg ggggagcgga cgatgaggat cgaccgtggg gggagcaagg caggcatggg
     1321 gcctgccccg acggtgtcat tgccgggcgt tccccccgcg caccggcggg gggcaacggc
     1381 attcgttccc ccgctcgtag gggcgacccc ccgtggtcgc cctcgtggcc cggcgcggtg
     1441 gcaacgggga cacgggggga tattggctgg cgttggtgtc gaatcggtgg tgaggcggcg
     1501 agggcagaca cggaggtctg cccctacagc ggtgagaatg cgcgctacgc cgccttgacc
     1561 tcgttggtca gcgccgccag cgacgccttg gcgtcgccga agagcatggc ggtcttgtcc
     1621 tcgtggaaca gctcgttctc gatgccggcg aagcccggcc gcatgctgcg cttgagcacg
     1681 atcacactgc gcgcctgatc gacgttgagg atcggcatgc cgaagatcgg gctgcccttg
     1741 tcgtggcgcg cggcggggtt gaccacgtcg ttggccccga ccaccagcgc cacgtcggcg
     1801 cgctcgaact cgtcgttgat ctgctccatc tcgaagagct ggtcgtaggg cacgttggcc
     1861 tcggccagca gcacgttcat gtgccccggc atacgcccgg ccaccgggtg aatggcgtac
     1921 ttgaccgtga cgccgcgctt ctccagctcg tcggccagct cgcgaatctg atgctgcgcc
     1981 tgcgccaccg ccaggccgta gcccggcacg atgatgacct cgttggcgta ggccatctgc
     2041 accgcgacgt cctcggcggt cgtctggcgg atcggcctgg cctccccggt cgcggccgcg
     2101 ccgcccgccg gcgccgagcc gaaggcgccg aacatcacgt tggccagcga gcggttcatg
     2161 gcccggctca tcagcacggt cagcagcgcg cccgaggcgc cgaccagcgc gccggccacg
     2221 atcaagatct ggttgtcgag cacgaagccg gccgccgccg ctgccaggcc ggtcatcgag
     2281 ttgaggaccg cgatcaccac cggcatgtcg gcgccgccga tcggcagcac caccagcacg
     2341 ccgaggatca gcgccgccgc cagcagcccc cagaccggca gcgtgttgct gccgagcagg
     2401 atgatcaccc agagcgccag gatggcgatc aggatgacgg cgttgacgat ctggcccccg
     2461 gcgaagcgga tcggccgccc ggagatcagc tcctgcagct tgccgaaggc gatcatgctg
     2521 ccggcgaacg agatggcgcc gatggtcgtg ccgaccacca ccgagagcga ctcgcccccg
     2581 ccgacgctgc cgctgtcgtt gtagaagcgc acgaactcgg cgaccgagac gagcgccgcg
     2641 gtgccgccac ccatgccgtt gaagagcgcc accatctgcg gcatggcggt catcgccacc
     2701 gtgcgcgcga agtaggtgcc gaccgccgcc ccggcgatca gcgccaccgc gatgatcgcg
     2761 atgttggcca cgttccagtg ccatgtttag tttggatcga tga
//
LOCUS       DASUBI010000291         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_547765, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000291 DASUBI010000000
VERSION     DASUBI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547765"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFU72_05520"
     CDS             complement(<1..341)
                     /locus_tag="VFU72_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5422979.1"
                     /translation="MAQSRQTDQRLQARIRTLFDEARLPVDVDVVDGVVHLVGRVDSP
                     RLHQAAIDLVEGLPGLSGIDDQIDYEVIAPDMATEPLDDDEQFGYADEEALDDDISDT
                     DPTFMEEVSTR"
     gene            453..857
                     /locus_tag="VFU72_05525"
     CDS             453..857
                     /locus_tag="VFU72_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5422980.1"
                     /translation="MASDAPEIMSIHQLEAASGVPARRIRHFIAEGLLPPPLGKGRAA
                     HYGRQHLELLRRIETLRAANLGLEEIRGRLEGELESSGGPAPESDTWRRWAIAPGIEL
                     HARADLGPELTRIAQTLADLARQMRGGGGRGG"
     gene            870..1340
                     /locus_tag="VFU72_05530"
     CDS             870..1340
                     /locus_tag="VFU72_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422981.1"
                     /translation="MREGETARDGWDERAVLRPVEVALEVAGFYAYLTLDQEARWMVA
                     SDSELGRVDVRVGRDGYELAVWDVSPGLFLEEEDERRRVAQERLARVTLPALARGYLA
                     ANQEVWWDEELHGVGARLRLELPFAAQPHLGEIARQRLDDLNDLIAFVETKLSE"
     gene            1360..3039
                     /gene="ade"
                     /locus_tag="VFU72_05535"
     CDS             1360..3039
                     /gene="ade"
                     /locus_tag="VFU72_05535"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEU5422982.1"
                     /translation="MIDRWHRVLAVAEGREPADLLLRDARLVNVASGEIERVAVALVA
                     GTIAGVGDYRAGEREIDLAGSYLAPSFIDAHVHVESSLLWVDAFARAVVPHGTGAVVT
                     DPHEIANVAGLPGVRALMGAATNQPLDLFFTIPSCVPASALESAGAEMTPAVIAEGLE
                     LPRVVGLGEMMNFPGVLAGDADIFAKLSLPAARRDGHAPGLRGHALNAYIASGIGSDH
                     ESTELEEAREKLRRGLKLMIREGSTERNLLELLPLVDDRTYPRCCFASDDRDCATLLH
                     DGHVDAILRKAIAAGLDPIRAIRMATLSSAEYWRLPWLGQVAPGYRANLVVFDRLDDL
                     RPRLVLYDGRVVAEDGRPAFEAPAPVPEWLTQTFHLAPVTPDDLRLAPHAPMIAVGAI
                     PGQIVTRKLEVEPTVREGLVVSDPERDLLKLAAVERHHGTGRVGVGLLAGFGLKRGAL
                     ATSVAHDAHNVVVTGVDDHDMLVAIGEVAAMEGGLVAVEHGRVLARLPLPVAGILSPR
                     PLEEVTVAYERLEAAAHELGSTLPAPFGLLSFMALSVIPEARVTDRGLVTF"
     gene            3050..3685
                     /locus_tag="VFU72_05540"
     CDS             3050..3685
                     /locus_tag="VFU72_05540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5422983.1"
                     /translation="MEAEREQRVEALVALARRWADDPAIAAARDGSNLVLGAGNPLAR
                     LVLIGEAPGGQEEKAGRPFVGPAGKLLDEAFAAAGLARDEVWITNVVKFRPTVPGVGN
                     RRRNRAPTRDESQLFLPWLREELAIIEPKALVCLGATAARAMIGQPVKIMSERGRWFT
                     SLGGRPTMLTYHPAFLLRRTTDYPERFAELASDLRTAGSFVEADDAPRAAR"
     gene            complement(3690..4235)
                     /locus_tag="VFU72_05545"
     CDS             complement(3690..4235)
                     /locus_tag="VFU72_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5422984.1"
                     /translation="MTREHDLSEQIILADGTLISVRPIRPADAPALQRFHRRPSPNSI
                     YLRHFSPVPELSDARARYFTHLETPDRFALVALDPEQPAEIIAVLRFDHEAGTDRAEY
                     AAAVEDRWQGRGIGRALTRRLIAAARERGIRQLYALVLPENRRMIHLLRDLGLPSRTV
                     WSDGALRVNVDLEPGEAEPPV"
BASE COUNT          612 a         1461 c         1671 g          639 t
ORIGIN      
        1 tcgcgcgtgc tgacctcttc catgaaggtc gggtcggtgt cggagatgtc gtcgtcgagc
       61 gcctcctcgt cggcgtagcc gaactgctcg tcgtcgtcga gcggctcggt cgccatatcg
      121 ggggcgatga cctcgtagtc gatctggtcg tcgatgccgg agagccccgg taggccctcg
      181 accaggtcga tggccgcctg gtgcagccgc ggcgagtcga cccggccgac caggtggacc
      241 acgccgtcga ccacatcgac gtcgaccggc aggcgggcct cgtcgaagag cgtgcggatg
      301 cgagcctgca gccgctggtc ggtttgtctc gattgcgcca tcagacactc ccctgctgtt
      361 gacgggcggc gcgcgatttg cccgtagaat acagtgcacg gctcgtaccc ggccaaccgg
      421 cctggcggac cgggcgaatg gaggcctgga gcgtggcatc cgacgcgcca gagatcatgt
      481 cgattcacca gctcgaagcg gccagcggcg tgccggcacg ccgcatccgc cacttcatcg
      541 ccgagggatt gctcccgcct ccgctgggca agggacgtgc cgcgcattac ggccggcagc
      601 atctggaact gctgcggcgc atcgagacgc tgcgcgcggc caacctgggg ctggaggaga
      661 ttcgcggccg gttggagggt gagctggaga gcagcggcgg cccggcgcct gagagcgata
      721 cctggcgccg ctgggcgatc gcgccgggga tcgagctgca cgcgcgggcg gacctgggtc
      781 cggagttgac gcgaatcgcc caaacgctgg ccgacctggc gcgccagatg cgaggcgggg
      841 gcgggcgcgg tgggtagatg aggagatcgg tgcgcgaagg ggagacagca cgagacggtt
      901 gggacgagcg ggcggtgctg cgcccagtcg aggtcgcgct ggaggtcgcc ggcttctacg
      961 cctacctcac gctcgatcag gaggcgcgct ggatggtcgc cagcgacagc gagctgggcc
     1021 gggtcgacgt gcgcgtcggc cgtgatggct acgagctggc ggtctgggat gtctcgcccg
     1081 gcctctttct ggaggaagaa gacgagcggc ggcgcgtggc gcaggagcgg ctggcgcggg
     1141 tcacgctgcc ggcgctggcg cgcggctatc tggctgcgaa ccaggaggtc tggtgggacg
     1201 aggagttgca cggcgtgggg gcgcgcctgc ggctggaact gcccttcgcc gcccagcccc
     1261 atctgggtga gatcgcgcgg cagcggctgg atgatctgaa tgacctgatc gccttcgtgg
     1321 aaacgaagct gagcgagtag atagggacgt gtgagtacgg tgatcgatcg atggcaccgg
     1381 gtgctggcgg tggccgaggg gcgcgagccc gccgatctcc tgctgcgcga tgcgcggctg
     1441 gtcaacgtgg cctcgggcga gatcgagcgc gtcgcggtgg cgctcgtcgc cggcacgatc
     1501 gccggcgtgg gcgactaccg ggcgggggag cgcgagatcg acctggccgg cagctacctg
     1561 gcgcccagct tcatcgacgc gcacgtccac gtcgagagct cgctcctctg ggtcgatgcc
     1621 ttcgcccgcg ccgtggtgcc gcacggcacc ggggcggtcg tcaccgaccc ccacgagatc
     1681 gccaacgtcg cgggtctgcc gggggtgcgc gcgctgatgg gcgccgcgac caaccagccg
     1741 ctcgacctct tcttcaccat cccctcctgc gtgccggcca gcgccttgga gagcgccgga
     1801 gcggagatga cgcccgccgt gatcgccgag gggctggagc tgccgcgcgt ggtcgggctg
     1861 ggcgagatga tgaacttccc cggcgtgctg gccggcgacg ccgacatctt cgccaagctc
     1921 agcctgccgg cagcccggcg cgacggccac gcgccggggc tgcgcgggca cgcgcttaac
     1981 gcctacatcg cctcgggtat cggctccgac cacgagtcaa ccgagctgga ggaggcgcga
     2041 gagaagctgc gccgggggct gaagctgatg atccgcgagg gctcgaccga gcgcaacctg
     2101 ctggagctgc tgccgctggt cgacgaccgc acctatccgc gctgctgctt cgccagcgac
     2161 gaccgcgact gcgccacgct gctgcacgac gggcatgtgg acgccattct gcgcaaggcg
     2221 atcgcggccg ggctcgaccc gatccgcgcc attcgcatgg cgaccctctc cagcgccgag
     2281 tactggcgcc tcccctggct ggggcaggtg gcgccgggct accgcgccaa cctggtcgtc
     2341 ttcgaccgcc tggacgatct ccggccgcgc ctggtgctct acgacggccg agtggtggcc
     2401 gaggacggcc gcccggcctt cgaagcgccc gcgccggtgc ccgaatggct cacgcagacc
     2461 tttcacctgg ccccggtcac gcccgacgat ctgaggctgg cgccgcacgc gccgatgatc
     2521 gcggtcggcg cgatccccgg ccagatcgtg acgcgcaagc tggaggtcga gccgaccgtg
     2581 cgcgagggtc tggtcgtctc cgaccccgag cgtgacctgc tcaagctggc ggcggtggag
     2641 cggcaccacg gcaccggacg cgtcggcgtg gggctcctcg ccggcttcgg gctcaagcgc
     2701 ggcgcgctgg cgacgtcggt ggcccacgac gcgcacaacg tggtcgtgac cggtgtggac
     2761 gaccacgaca tgctggtggc gatcggggag gtggccgcga tggagggcgg gctggtggcg
     2821 gtggagcacg ggcgcgtgct ggcacggctg ccgctgccgg tggcgggcat cctctcgccg
     2881 cgcccgctgg aggaggtcac ggtggcctac gagcggctgg aggcggcggc gcacgagttg
     2941 gggagcacgt tgccggcgcc cttcggcctc ctctccttca tggcgctctc ggtcatcccg
     3001 gaggcgcgtg tcaccgatcg cgggctggtg accttctagg gaggcgcgga tggaagcgga
     3061 gcgcgagcag cgagtagagg cgctggtggc tctagctcgg cgctgggcgg acgatccggc
     3121 gatcgcggcg gcacgggatg gcagcaacct ggtgctgggc gccggcaacc cgctggcccg
     3181 cttggtcctg atcggtgagg cgccgggggg gcaggaggag aaggccgggc ggccgttcgt
     3241 cggcccggcg ggcaagctgc tggacgaggc gttcgccgcc gccgggctcg cgcgcgacga
     3301 ggtctggatc accaacgtgg tcaagttccg cccgaccgtc cccggcgtgg gcaaccgcag
     3361 gcgcaaccgc gccccgacgc gcgacgagtc gcagcttttc ctcccctggc tgcgcgagga
     3421 gctggcgatc atcgagccga aggcgctggt ctgcctgggc gcgaccgcgg cgcgagcaat
     3481 gatcggccag ccggtcaaga tcatgtccga gcgcggccgg tggttcacca gcctgggcgg
     3541 acggccaacg atgctgacct atcacccggc cttcctgctg cgccgcacca ccgattaccc
     3601 cgaacgcttc gccgagctgg cgagcgatct gcgcaccgcc ggctccttcg tcgaggccga
     3661 cgacgcgccg cgcgcggcgc gctgacggtt cagacaggcg gctccgcctc ccctggctcg
     3721 aggtcgacgt tgacccgcag cgcaccgtcg gaccagacgg tgcggctcgg cagaccgaga
     3781 tcacgcagga ggtgaatcat gcgccggttc tcggggagta ccagcgcata gagctggcgg
     3841 ataccgcgct cgcgtgccgc cgcgatcagc cgccgggtta gcgcgcggcc gataccgcgc
     3901 ccctgccagc ggtcctcgac ggccgcggcg tactcggcgc ggtcggtgcc cgcctcgtgg
     3961 tcgaagcgca gcaccgcgat gatctcagcc ggctgctcgg ggtcgagcgc gaccagggcg
     4021 aagcggtcgg gggtctccag gtgggtgaaa tagcgggcgc gggcgtcgga gagctccggc
     4081 accgggctga agtggcgcag gtagatcgag ttcgggctgg ggcgccgatg gaagcgctgc
     4141 agcgccgggg cgtcggcggg ccggatcggc cgcaccgaaa tcaaggtgcc atcagcgagg
     4201 ataatctgct cgctcagatc atgctcacgc gtcatacgtc accgacccaa ctggcggcct
     4261 ctggttcgac cgccgcttcg agtttacgtt tctgccggct gcctggatcg tgggcccgtg
     4321 ggtgagaaga gacgaggaga ttcgtcatgc ctgacgcgcg gctgcgcgcg ccggaagagc
     4381 gcg
//
LOCUS       DASUBI010000292         9293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_499248, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000292 DASUBI010000000
VERSION     DASUBI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499248"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFU72_05550"
     CDS             <1..401
                     /locus_tag="VFU72_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5422985.1"
                     /translation="EEVARAMDVPLEKVRQVLDAARHPVSLEAPVGQEGDAFLGDFIE
                     DETMPPPLELASHKLLRGQIGDTLDRLTERERRIIVLRYGLDDGQFRTLEEVGREFGI
                     TRERIRQIEAKALRKLRHPQYSRQLRGYLD"
     gene            complement(551..1813)
                     /gene="fabF"
                     /locus_tag="VFU72_05555"
     CDS             complement(551..1813)
                     /gene="fabF"
                     /locus_tag="VFU72_05555"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5422986.1"
                     /translation="MRVANRERSPSERVVITGLGVISPVGNDPAVAWNALLEGRSGTG
                     PITRFDATGFDSTIAGEVKDFDPREHLAAKAARRVDRYVQFAVAAARQALLGAGLTIS
                     EANRDRIGVLIGTALGGIETAERELRTLSERGPGRVSPFFIPMFLGDMGAGYVAIELG
                     ARGPNFATLSACASGAHALGEATEIIRRGAADVMLAGAAEAAVTPGSVAGFAALGALS
                     TRNDEAQTASRPFDATRDGFVVAEGAAVLVLERLDYAEARGARPLAELSGYGATADAN
                     HITQPAPGGVGAAEAMRGAIAGAGLTPVQIDYINAHGTSTPLNDRLETEAIKRVFETT
                     PTPVSSTKALTGHLVGAAGALEAVFSIQSLSQQTIPPTWHLRVPDPACDLDYVSEGPR
                     AARLRHVLSNSLGFGGHNVALVFSRVEE"
     gene            complement(1800..2780)
                     /locus_tag="VFU72_05560"
     CDS             complement(1800..2780)
                     /locus_tag="VFU72_05560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU5422987.1"
                     /translation="MRIAVTRLIPESGLELLRAEADVALWPNPLPPGRDEIITLAHGA
                     DGLLTLVTDRVDGALLDALPSVRVVSNYAVGFDNIDVPACTARDVAVCTTPDVLTDAT
                     ADYGFTLLLAAARKLIPAVEAARRGDWLTWEPMGFLGQETAGRALGVVGLGRIGAALA
                     RRGHAFGMRIVYTDAVARPELEQELGAERRELDALLAESDFVSVHVPLTPDTVHLIGA
                     RELKLMKPSAVLVNTSRGPVVDNEALADALEQGVIWAAALDVTEPEPLPATHRLMHLP
                     NVIVTPHIASATYDTRSEMSILSARNVLAVLQGQEPPRCLNPEVLGRARR"
     gene            3127..4167
                     /locus_tag="VFU72_05565"
     CDS             3127..4167
                     /locus_tag="VFU72_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5422988.1"
                     /translation="MTIGERLRSARRTLDPEGINTTIIEQARQHLPDVQAACGALGFF
                     GATRLVVAIDLFGEAKSNNQSGQAQSGAARSGRGSAGKRGRGANAAADDAVVALLAGV
                     PSTTELVVVERSFDTQAEQLTASSGARVAVERHDVPRGQPLLQWLVARARQHDTVIEA
                     PIAEEILNALFPGSWQSSARFDDVPPDLFRLDNEIAKLSAAAGTGPVTTDLVRQLVLG
                     ADAENTWGLVDALTGGQPSRAVQEVERALSHGTAPEALLGQLTGHFEALAAILLAGRG
                     TSPREIAAATGLSDGRVRMASSGAQRTSIAAVAGAIDALRTLDADAKRGRVEPADALV
                     ALVARLASGESR"
     gene            complement(4561..4836)
                     /gene="rpsT"
                     /locus_tag="VFU72_05570"
     CDS             complement(4561..4836)
                     /gene="rpsT"
                     /locus_tag="VFU72_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5422989.1"
                     /translation="MANSKSAAKRARVAERQRIRNRIFRSAARTYVKKAERLIAARDA
                     EAASTAVGDAISTLDRAAIKGIIHKNNAARRKSRLMAKFNQLVRPQA"
     gene            4991..6631
                     /gene="murJ"
                     /locus_tag="VFU72_05575"
     CDS             4991..6631
                     /gene="murJ"
                     /locus_tag="VFU72_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU5422990.1"
                     /translation="MTSISRRPRRVNPPSREQAGGRSVAANASIVAGAFVVSRVLGLL
                     REVLIARQFGTSQNYDAYVAAFKIPDFLFLVVMSGAFGAAFIPVFAGFLSRDERERAW
                     HLSSAVISYTVVTLVGVGLLVFVFAGPLMRYVVAPGYTPAQEHLATNLTRLLLLSPLL
                     LGLGAASKGMLEAQDAFTVPAIAPILYNLGIIFGALFLAPSIGVYGLVVGVIAGACGH
                     AGIQFVTLARRGMRFRPTLSTHVEGLRAVGHLMLPRLVGQAAFQVNFIVMTNFASRLP
                     QSPVAALNYAYQLFMLPHGVLALSLSTVIFPAMARQYELGRVRELKQTLTSALGPLLF
                     LTIPASIGLFAFRVSIVQVIFQHGDFDAHSTAMVARGLEFFAIGLVAFAVVEAVTRAF
                     YAMHDTRTPVIAGVTAVIANIGLSALLAPKLGHGGLALSISLTTFLEMTILLVVLARR
                     MGGFDSQLPRQLLKMLVAGAVLYLVSRWLAGPLAAATNPANGHRLVTILAFGVTLAAA
                     GATYFVAAYYLRVPELERVLARVRPRLPGWAARWLRPL"
     gene            6674..8500
                     /gene="lepA"
                     /locus_tag="VFU72_05580"
     CDS             6674..8500
                     /gene="lepA"
                     /locus_tag="VFU72_05580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5422991.1"
                     /translation="MSDQSLIRNFSIIAHIDHGKSTLADRLLEYTHTISEREMVAQVL
                     DSMDLEREKGITIKARAVRMEYTAADGQTYHLNLIDTPGHVDFTYEVSRSLAACEGAL
                     LVVDAAQGIEAQTMANVHLAVDGGLALIGVINKIDLPNAEPTRVKREVGEFIGLLDQE
                     IVLASAKTGEGIAEILEAIVKQVPPPGGDPDRPLRALIFDSHYDPYRGVIAYVKIVDG
                     TLRGDDRVRMMVTGKDAELLEIGMFRPLMTPVAALGTGEVGYIATGLKNVRDCQVGDT
                     VTLASAPAQTPLPGYRPIKPMVFAGFYPVNGEEYPELRDALERLKLNDASLVFEPESS
                     DALGFGFRCGFLGLLHMEIVQERLEREYDIDLLATAPSVEYEVVMMDDSVRTVDNPSE
                     MPNPGTILEIREPWVDLTIIAPSRYIGTIMELVTTRRGEFRDMDYLDEGRVRQSFGMP
                     LGELIVDFYDQLKSRTQGYASLDYQLAGMRPADLVKLDVLVNGTSVDALSLITHRDNA
                     YYRGRALVQQLKELIPRQMFDVPVQASIGSRIISRETIRALRKNVLAKCYGGDVTRKR
                     KLLEKQKEGKARMKMVGSVEIPQEAFLAVLSIGDEARGNGRE"
     gene            8497..>9293
                     /locus_tag="VFU72_05585"
     CDS             8497..>9293
                     /locus_tag="VFU72_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5422992.1"
                     /translation="MSSAHEILVVGLGPGSPDLLTRETVAALRSSRVFLRTHIHPTLA
                     YLPESAGWRSFDRLYDEAPDFSALYDRMVDTLLAKSRQGPLVYAVPGSPLFAEASVRH
                     LRRRAALEGVPVRVLPAVSFIDVVAGALDLDPVAGNIQLVDALDLAAYSEREPFAGGS
                     LPLSNLRPALVAQLYSKPIASAAKLALLTVYPPDLQIALVTAAGTPEQAIARRALSEL
                     DHGPVDHLSTLYLPTVEPLVWPRSPEAVRQITSRLRAPGGCPWDREQT"
BASE COUNT         1443 a         3280 c         3004 g         1566 t
ORIGIN      
        1 acgaggaagt cgcgcgcgcc atggacgtgc cgctggagaa ggtgcgtcag gtgctcgacg
       61 ccgcgcgcca ccccgtctcc ctggaggcgc ccgtgggcca agagggcgac gcgttcctgg
      121 gtgatttcat cgaagacgaa acgatgccgc cgcccctgga gttggcctca cacaagctcc
      181 tgcggggtca gatcggcgac acgctcgacc gcttgactga gcgcgagcgg cgcatcatcg
      241 tcctgcgcta cgggctcgat gatggtcagt ttcgtaccct ggaggaggtc gggcgcgagt
      301 tcggcatcac gcgcgagcgc atcaggcaga tcgaggccaa agccctgcgc aagctgcgcc
      361 accctcagta cagtcggcag ctccgcggct atcttgacta ggctctagtt caccgccgat
      421 ggacagcgcg gcgagtcatc gccgcgctgt ttcgtatctc tgcatttgga gatgctttcc
      481 agtgggtccg tggcgtgctg tgttccaaga gtcctggctt gacgtgacca agcgcccccc
      541 gcgagagccc ctactcctca actcgcgaaa acaccagcgc aacattatgt ccaccaaacc
      601 ccagcgagtt gctgagcaca tggcgaagac gcgccgcgcg cgggccctct gagacatagt
      661 cgagatcgca ggctgggtca ggtactctga gatgccaggt gggcgggatc gtctgctggc
      721 tcaaggactg gatggagaag accgcttcca gcgcgccggc cgcgccgacc aaatgtccag
      781 tcagcgcctt ggtcgagctg accggcgttg gtgtcgtctc gaagacgcgc ttgatcgcct
      841 cggtctccaa acgatcgttg agtggcgtcg aggtgccgtg agcgttgatg tagtcgatct
      901 ggaccggcgt caatccggcg ccggcgatcg ccccgcgcat cgcttccgcg gcgcccactc
      961 cgccgggcgc cggctgggtg atgtggttcg cgtcggcggt cgctccatag ccactgagct
     1021 cggccagcgg ccgcgcaccg cgcgcctcgg cgtagtcgag ccgctccagc accagtaccg
     1081 ccgcgccctc ggcaaccacg aaaccgtcac gcgtagcgtc gaacggtcgg ctggcggttt
     1141 gcgcctcatc gttacgtgtc gagagcgcgc ccaacgcggc gaaccccgcg accgagcccg
     1201 gcgtcaccgc tgcctccgcg gcgccggcca gcatcacgtc ggccgcgcct cgccggataa
     1261 tctccgtcgc ttcgccgaga gcatgcgcgc cgctggcgca ggccgagagc gtggcgaagt
     1321 tggggccgcg cgcgcccagc tcgatggcga cgtagcccgc gcccatatcg cccagaaaca
     1381 tcgggataaa gaagggactc acacggcccg gcccgcgctc ggacagcgtg cgcagctcgc
     1441 gctcggccgt ctcgatgccg ccgagcgccg tgccgatcaa gaccccgatg cggtcacggt
     1501 tcgcctctga tatcgtcagc ccggcgccga gcagcgcctg gcgcgccgca gccaccgcga
     1561 attgcacgta gcgatcgacc cgccgcgctg ctttggccgc cagatgctcg cgcggatcaa
     1621 aatccttcac ttccccggcg atggtcgagt cgaaaccggt ggcatcgaag cgcgtgatcg
     1681 gtcccgtgcc cgagcgtccc tccagtagcg cgttccaggc caccgcgggg tcattcccaa
     1741 ccggcgaaat cacgcccaga cccgtgatga cgacacgctc gctgggacta cgctcccgat
     1801 tagcgacgcg cacggccgag cacctccggg ttcaggcaac gcggtggctc ctgtccttgc
     1861 agcactgcca gcacgttgcg ggcggagagg atcgacatct cggagcgggt gtcgtaggtg
     1921 gcgctggcga tgtgcggcgt gacgatgacg ttcggcaggt gcatcagtcg atgcgtcgcc
     1981 ggcagcggtt ccggctcggt gacgtcgagc gccgcggccc agatgactcc ctgctccagc
     2041 gcgtccgcca gcgcctcatt gtcgaccacc ggcccgcgcg aggtattgac cagcaccgcg
     2101 ctcggcttca tgagcttgag ctcgcgcgcg ccgatcaaat gtacggtgtc tggcgtcagc
     2161 ggcacgtgta cgctgacgaa gtcgctttcg gccaggagcg catccagctc gcgccgctcc
     2221 gcgccgagct cttgctccag ctccggacgg gcgaccgcgt cggtgtagac gatgcgcatg
     2281 ccgaaggcat gcccccgtcg cgcgagcgcc gccccgatgc gccccagccc gactaccccg
     2341 agcgctcgtc ccgccgtctc ctgccccagg aaacccatcg gttcccaggt caaccagtcg
     2401 ccacggcgcg cggcctcgac cgccggaatc agcttgcgcg ccgccgccag caacagcgtg
     2461 aagccgtagt cggcggtcgc atcggtgagg acgtcgggcg tcgtacagac ggccacgtcg
     2521 cgcgcggtgc aggccggtac gtcgatgttg tcgaagccga ccgcgtagtt gctcaccacc
     2581 cgaaccgacg ggagcgcgtc gagcaacgcg ccgtcgacgc ggtccgtaac cagtgtcagg
     2641 aggccgtcgg cgccatgcgc gagcgttatt atctcgtcgc ggcctggcgg cagcggattc
     2701 ggccagagcg cgacgtccgc ctccgcgcgc agcaattcca atccactctc cgggatcagc
     2761 cgcgtgaccg cgattcgcac gcgcgcctcc tttcgctgcg cgcattctag cccggctgaa
     2821 ggcgtgccgg caaagcgcgc gaacgtacgg taagcgcgct ctgggcaagc cgtcttggct
     2881 gtgctatact cacgcctggg gcaaggcccc acgcagcccg cgacgtgatg tacggtcttg
     2941 agccaacgta tgccaatcaa cgggagctgc taatcaagcc tcgtggggtt tggggttgcg
     3001 gcgcccacct gctatgcagg ttcaacactc gaatccgtcg cggaacgtgg gctgaggccc
     3061 ctttctcttt gccctgtgat cggcaagcga gcgcgcgtga tcacgatcct gcacgggaca
     3121 gaccagttga cgatcggcga gcgcctgcgc agcgcccgac gtaccctcga ccccgaaggc
     3181 atcaatacca ccattatcga acaagcgcgg cagcatcttc cagacgtcca ggcagcctgc
     3241 ggcgccctcg gcttcttcgg cgccacccgc ctggtcgtag ccatcgacct ctttggcgag
     3301 gcaaagtcga acaaccagtc cggccaagca caatccggcg ccgcgcgctc gggacggggc
     3361 agcgcgggca agcgtggccg cggggcgaac gcggccgcgg acgacgcggt cgtcgctctg
     3421 ctagcgggag tgccgtcgac cacggagctt gtggtggtcg agcgctcgtt cgacacgcag
     3481 gccgagcaac tgaccgcatc gtccggggcg agagtcgccg tcgagcgcca tgacgtgccg
     3541 cgtgggcagc cactgctgca gtggctcgtc gcccgtgccc ggcagcatga cacggtgatt
     3601 gaggccccga tcgccgagga gatcctcaac gcgctctttc ccggcagctg gcaaagcagc
     3661 gcgcgcttcg atgacgtgcc gcccgacctg ttccgcctcg acaacgagat cgccaaactc
     3721 tccgcggcgg caggtacggg gccggtcacg accgacctcg tccgtcaact ggttcttggg
     3781 gcagacgctg agaacacctg ggggctggtc gacgctctga ccggtggcca gccgtcacga
     3841 gcggtgcaag aagtggaacg cgcgctctcg cacggcaccg cgccggaggc gctgttgggg
     3901 cagttgaccg gccacttcga ggcgctcgcg gcgatactcc tcgcgggccg aggcacatct
     3961 ccacgcgaga tcgcggccgc gactggcctc tccgacggtc gggtgcgcat ggcctccagt
     4021 ggcgcgcagc gcacgtcgat cgccgcggtg gccggagcaa tcgacgctct gcgtaccctc
     4081 gatgccgacg ccaagcgcgg ccgggtcgag ccggccgatg ctctggtcgc actcgtggca
     4141 cggctggcaa gcggggagtc gcggtagtcg cctcgcttgt cgcgtcagtg cttgctgcga
     4201 gcttgtcgaa ggggggtagg gataccggcg ccgtcttccc agctaacctg agccctcccc
     4261 ccgtcatcct gagccgcggc gaagcattcc gcatcagcga caagcggcag tgctgccgca
     4321 actgagagga gattcatcgg ctgcggcctc agcatgacag ggaggacgta tctctcctac
     4381 ccctccctaa tcaccaacga tccaggatgg tgttcatccc gcccgagcca gcaaagctct
     4441 gctccgagcg tgacgttcgc actcataggt tatgccgtat cggcgccgca tgtcctggag
     4501 acagcactag ccggagcgct gccgctccgg ctctctcgct cggtgtggct tcgcgcgctg
     4561 ctacgcctgc gggcgcacca attgattgaa cttcgccatc aggcgcgact tgcgccgtgc
     4621 ggcgttgttc ttatgaatga tccctttgat cgccgcccgg tccaacgtgc tgatcgcatc
     4681 gcccaccgcg gtcgaggccg cctcggcgtc ccgcgcggcg atcagccgct ccgccttctt
     4741 gacgtaggtc cgcgccgcgg agcggaagat ccgattgcgt atccgctggc gctccgccac
     4801 ccgcgcccgc ttggctgctg acttgctgtt cgccaaagct ctagatcctc cgataggtgc
     4861 tcaaaccggt caaattgtct cacacgcaga caaaattggc agcgcatctg cctgccgcgt
     4921 ttgaggatac caagcgccga gcggccgcgc aagcgacggc acccctcccg gttgcgtaca
     4981 attccggctc atgacgagca tctcccggcg cccccggcgc gtcaacccac catcacggga
     5041 gcaggccggc ggccgctcgg tcgcggctaa tgcctcgatc gtggccggcg ccttcgtcgt
     5101 cagccgcgtg ctggggctct tgcgcgaggt gctgatcgcg cgccagttcg gcaccagcca
     5161 gaattacgac gcctacgtcg cggcgttcaa gatccccgat ttcctcttcc tggtggtgat
     5221 gagcggggcc ttcggcgcgg ccttcatccc ggtcttcgcc ggcttcctca gccgcgacga
     5281 gcgcgagcgc gcctggcacc tctccagcgc ggtcatcagt tacaccgtcg tgaccctggt
     5341 tggcgtcggt ctgctggtct tcgtcttcgc cgggccactc atgcgctacg tcgtcgcccc
     5401 cggctacact ccggcccagg agcacctggc caccaatctc acgcgcctcc tcctgctctc
     5461 gccgctcctg cttggcctcg gcgccgccag taaaggcatg ctcgaggcgc aggatgcctt
     5521 caccgtgccg gcgatcgcgc cgatcctcta caacctcggc atcatcttcg gagccctctt
     5581 cctggcaccc agcattggcg tctatggtct ggtggtgggc gtcatcgccg gcgcctgcgg
     5641 ccacgccggc atccagttcg tcaccctggc gcggcgcggc atgcgcttcc gccccaccct
     5701 atccacccat gtggagggct tgcgagcggt cggacacttg atgctcccgc gcctggtcgg
     5761 gcaggcggct ttccaggtca acttcatcgt catgaccaac ttcgcctcgc gcctgccgca
     5821 gagcccggtg gccgcgctca actacgccta ccagctcttc atgctgcccc acggcgtgct
     5881 cgccctcagt ctgtcgacgg tgatcttccc ggccatggcg cgccaatacg agctcggccg
     5941 cgtgcgcgag ctgaagcaga cgctgacctc cgccctcgga ccgctcctgt tcctgaccat
     6001 cccggcttcg atcgggctct tcgccttccg ggtcagcatc gtgcaggtca ttttccagca
     6061 cggggacttc gacgcccact cgaccgccat ggtcgcgcgc ggcctggagt tctttgcgat
     6121 cggtctggtc gccttcgccg tagtcgaggc cgtcacgcgc gccttctatg ccatgcacga
     6181 cacccgcacc ccggtcatcg ccggggtcac ggcggtgatc gcgaacatcg gcctgagcgc
     6241 gctgctggca ccgaagctcg gccacggcgg cctggcgctc agcatctcgc tcaccacgtt
     6301 cctggagatg acgatcctgc tggtcgtgct ggcgcgccgc atgggcgggt tcgatagcca
     6361 gcttcccagg cagctgctca agatgctggt ggccggcgcg gtgctctacc tggtctcgcg
     6421 ctggctggcc ggtccactgg ccgcagcgac caaccccgcc aatggtcacc ggctcgtgac
     6481 gatactcgcc ttcggcgtta ccctcgcggc cgccggcgcg acctacttcg tcgcggccta
     6541 ttacctgcgc gtccccgagt tggagcgggt gctcgcgcgc gtgcgccccc ggctcccggg
     6601 ctgggcggca cgctggctcc gtccgctata attggcgact ggcctacgac ggggagaagg
     6661 cagcacgcga agcatgagcg accagagtct gattcgtaac ttcagcatca tcgcacacat
     6721 cgatcacggc aagtcgacgc tcgccgaccg gctgctggag tacacccaca ccatcagcga
     6781 gcgcgagatg gtcgctcagg tgctcgacag tatggatctc gagcgcgaga agggcatcac
     6841 gatcaaggcg cgtgcggtgc gtatggagta caccgccgcc gacggccaga cctaccacct
     6901 gaacctgatc gacaccccgg ggcacgttga tttcacctat gaggtgagcc gcagcctggc
     6961 cgcctgcgag ggcgcccttt tggtggtcga cgccgcgcaa ggcatcgagg cccagaccat
     7021 ggccaacgtt cacctggcgg tcgacggcgg gctggcgctg atcggcgtga tcaataagat
     7081 cgacctgccg aacgcggagc cgacccgcgt caagcgcgag gtgggcgagt tcatcggcct
     7141 gctcgatcaa gagattgtgc tggcctcggc caagaccggc gaaggcatcg ccgagatcct
     7201 ggaggcgatc gtcaagcagg tgccgccgcc agggggtgat cccgaccgtc cgctgcgcgc
     7261 gctcatcttc gactcgcact atgaccccta tcgcggtgtg atcgcctacg tcaagatcgt
     7321 cgatggcacg ctgcgcggcg acgaccgcgt gcgcatgatg gtcacgggca aggacgcgga
     7381 gctgctggag atcggcatgt tccgcccgct gatgaccccg gtggccgcgc tgggcaccgg
     7441 cgaggtcggc tacatcgcca ccgggctgaa gaacgtgcgt gattgccagg taggtgacac
     7501 ggtcacgctg gccagcgccc cggcgcagac gccgctgccg ggctatcgcc cgatcaagcc
     7561 gatggtcttc gccggcttct acccggtcaa cggcgaggag tatcccgagc tacgcgacgc
     7621 gctggagcgg ctcaagctca acgacgcctc gctcgtcttc gagccggagt cgtccgacgc
     7681 gctcggtttc ggcttccgct gcggcttcct gggcctgctg cacatggaga tagtgcagga
     7741 gcggctggag cgtgagtacg acatcgatct gctggcgacc gcgcccagcg tcgagtacga
     7801 agtcgtgatg atggacgact cggtgcgcac ggttgacaac ccctcggaga tgccgaaccc
     7861 cggcacgatc ctggagatcc gtgagccctg ggtcgacctg acgatcatcg cgccctcacg
     7921 ctacatcggc acgattatgg aactggtcac gacccgccgc ggcgagttcc gcgacatgga
     7981 ttatctcgac gaggggcgcg tgcgccagag cttcggcatg ccgctcggcg agttgatcgt
     8041 cgacttctac gaccagctca agtcgcgcac acagggctat gccagccttg actaccagct
     8101 tgctggcatg cgcccggccg acctggtcaa gctcgacgtg ctggtcaatg gcacctcggt
     8161 cgacgcgctg tcgttgatca cccaccgcga caacgcctac tatcgcgggc gcgcgctggt
     8221 gcagcagctc aaggagctga tcccgcgaca gatgttcgac gtgccggtgc aagcctcgat
     8281 cggcagccgc atcatctcgc gcgagacgat ccgcgccctg cgcaagaacg tgctggccaa
     8341 gtgctacggc ggcgacgtga cgcgcaagcg caagctgctg gagaagcaga aggagggcaa
     8401 ggcacgcatg aagatggtcg gcagcgtcga gatcccccag gaggccttcc tggcggtgct
     8461 gagcatcggc gacgaagcgc gcgggaacgg ccgcgagtga gcagcgctca cgagatcctg
     8521 gtcgtcggcc tcggccccgg ttcgcccgac ctgctgaccc gggagacggt cgcggcgctg
     8581 cgctcgtcgc gggtcttcct gcgcacccac atccacccga cgctcgccta cttgcccgag
     8641 tccgccggct ggcgctcctt cgaccggctc tacgatgagg ccccggactt cagcgcgctc
     8701 tacgaccgca tggtggatac gctgctggct aagtcgcggc agggcccatt ggtctatgcc
     8761 gtccccggca gcccgctctt cgcggaggcg agcgtgcgcc acctgcgccg gcgggcggcg
     8821 ctcgaaggcg tgccggtgcg cgtgctgccg gcggtcagct tcatcgatgt ggtcgccggc
     8881 gcgctcgatc tcgaccctgt cgccggcaat atccagttgg tcgacgcgct cgatctcgcg
     8941 gcgtactccg agcgcgagcc gttcgccggc ggctcactgc cgctctcgaa tctgcgcccg
     9001 gccctggttg cccagctcta ctcgaagccg atcgcctcgg ccgccaagct ggcgctgttg
     9061 actgtctacc cgcctgacct tcagatcgcg ctggtcacgg ctgccgggac gccggagcag
     9121 gcgatcgcgc gccgcgcgct cagcgagctg gatcacggtc cggtcgacca cctctcgaca
     9181 ctctatcttc cgacggtcga gccattggtc tggccacgct cgcccgaggc cgtgcggcag
     9241 atcacttccc ggctccgcgc cccgggcggc tgcccctggg accgcgagca gac
//
LOCUS       DASUBI010000293         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_413057, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000293 DASUBI010000000
VERSION     DASUBI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413057"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFU72_05590"
     CDS             complement(<1..288)
                     /locus_tag="VFU72_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422993.1"
                     /translation="MAPVRSSDASVVAPQRGFPRLVNLALVLLVAASLIAGMLVMSQA
                     MPRAAVVEQQAELAAAWPLLTIEESLAAWRAVGDRYRTTGETPVDWPLPAAP"
     gene            complement(300..800)
                     /locus_tag="VFU72_05595"
     CDS             complement(300..800)
                     /locus_tag="VFU72_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422994.1"
                     /translation="MASPWPYPTLTVAPRPTAIGASTALASPRAATPDTLPPDQAALL
                     ELVIAGLDAAITSQPLPAELSPVVRQQLLALIERQDPYYQRRAWVVDWQPGPLTWTSG
                     PAPGPALAVWHVALTGAVTLVTSDHLAPVVTAFDPSVNDLSVTLARDGDRWTVARVAG
                     ALFPTP"
     gene            complement(1074..1292)
                     /locus_tag="VFU72_05600"
     CDS             complement(1074..1292)
                     /locus_tag="VFU72_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422995.1"
                     /translation="MTERPVARTGVGDYLRRWGRLELLFLTLAALGILTCGCVALVLL
                     WRLGLAQAGPTPTLGWPAAPTLAPRPTP"
     gene            complement(1285..3018)
                     /locus_tag="VFU72_05605"
     CDS             complement(1285..3018)
                     /locus_tag="VFU72_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5422996.1"
                     /translation="MSLGSGLRRLALLSTLLTLLGALLAPAALAAAPTPTPHPATSTP
                     APRPTATVAGTALVDGVSYIAVLDTAGGRKQYDKWHREMYSYGVPDPDAARIAFAWVK
                     DWCQTKVPAGCPATGFDLSAVDVRDGSAGNGFNDQCQVWGVDFTPQWGDTTPKPAPKC
                     APDAPKRDGLNGARATSCGSTAASGVCAGGGASDGGSYPGLSATTDQPATGSFCVTLP
                     ANLGEACTDIRLPIPTVGSVVRAALGGLAAADWGELLGALLRGLVRLLVNSLVQATGQ
                     PVLDGLLHNPDLHAGGGGLANVRSVFLALRDAAVALCLAVFAAAALQYLYGYLPDPGS
                     LLGRLVAVLAAIGFYPLLMDEFLAGINTLIDGVLAAGQDEVNGVWVKLLALTTLPAAA
                     PGIVLVLALVAGLLLLTLLFLKLAAVATLVLLYVVGPLALAGALHPVTARVAALWAQA
                     LVRVACWPLAWALELRLLWALMQGINPLSGGAVADAMTLVGLLMVMAATPFALSKVSA
                     GEHVRGAVAKVVGVTTLAAGAAVGGVGGTAAAAGHAGAGWTGVVRGIGAASAVRQQAQ
                     ARQAAGASGDD"
     gene            complement(3031..>3600)
                     /locus_tag="VFU72_05610"
     CDS             complement(3031..>3600)
                     /locus_tag="VFU72_05610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5422997.1"
                     /translation="AANGFACGAAYAEGILALLAAVARPPVARAGFWAITGGRTYPIT
                     QEFGRTDFSTGSGRDNYGYGAAYGLPADAHPGIDVGTPAGTPLASPVDGVVEIAGGSG
                     YYRDEDEPGVDPATSGELRLRRANGDLVILGHLRRITVRVGQVVRASQPVGVSGSANG
                     PHVHVEVRVVDPALASGYRVVDPRAYLGE"
BASE COUNT          563 a         1307 c         1274 g          456 t
ORIGIN      
        1 cggtgccgct ggcaacggcc agtccaccgg cgtctctccg gtcgttcggt agcggtcccc
       61 gaccgcccgc caggctgcca ggctctcctc gattgtcagg agcggccagg ccgcagcaag
      121 ttccgcctgc tgttctacca ccgcagctct gggcatggcc tgggacatca ccagcattcc
      181 ggcgatgagc gatgcggcaa ccagcaggac gagcgcgaga ttcaccaggc gtgggaaccc
      241 gcgctgcgga gcaactactg aggcatcgct cgagcgtacc ggtgccatga acggcctcct
      301 cacggagtag gaaagagtgc gccggcgacg cgcgcgaccg tccaccggtc gccgtcccgg
      361 gccagtgtga cactgaggtc gttgacgctc gggtcaaacg cggtcaccac cggcgcgaga
      421 tggtcgctgg tgaccagcgt gaccgccccg gtcagcgcga catgccagac ggccagcgcc
      481 gggccgggcg cgggaccact cgtccaggtg agcggccccg gctgccagtc caccacccag
      541 gcacgccgct ggtaataggg gtcctggcgc tcgatcagcg ccaggagttg ctgccgcacg
      601 acgggcgaca actctgccgg cagcggctgg ctggtgatgg ccgcgtccag gcccgcaatg
      661 accagttcta agagcgccgc ctggtcgggc ggcagcgtgt ccggtgtggc cgcacgtgga
      721 ctggcgagag cggtcgatgc accgatggcg gtagggcgag gcgcaacggt gagcgttgga
      781 tacggccagg gggacgccac cgaatccggc gtcccgacgg cccctgttac cgaggtcgca
      841 ataaggccag aagcgctcgc cggcgtccag gcaaggccgc cgccgagagc cggcgtggat
      901 gtgcccgccg gagtttccat cggagcagtc gccgctagcg ccgctccacg ttgctgctgg
      961 tgcgccagca ccccgctaat gagaactagt atcaatagca tccccgccgc gatgaggagc
     1021 ggcccgggaa caccgggcgc gccgtagacg tcgtgaaaca tcggcaccct ccgtcacggc
     1081 gtgggccgcg gcgccagggt cggcgccgcc ggccagccga gcgtcggggt cggacccgcc
     1141 tgtgcgaggc ctaggcgcca cagcaacacc agcgccacac agccgcaggt caggattccg
     1201 agcgcggcca gggtcaggaa gagcagttcc agccggcccc agcggcggag gtagtcgccc
     1261 acgccggtgc gcgcgaccgg tcgttcagtc atcgccgctc gctcctgccg cctggcgcgc
     1321 ctgcgcctgc tggcgcaccg cgctggccgc gccgatccca cgcaccacac cggtccagcc
     1381 cgcgccggcg tgaccggcag cggcggccgt cccgcccacg ccaccgacgg ccgccccggc
     1441 ggccagggtc gtcacgccga ccactttcgc caccgcgccc cggacgtgtt caccggcgct
     1501 gaccttgctg agggcgaagg gcgtcgcggc catgaccatg agcaggccga ccagcgtcat
     1561 ggcgtcagcc acggctccgc cactgagtgg attaatgccc tgcatgagcg cccacaagag
     1621 gcgcagctcg agcgcccagg ccaggggcca gcaggccacg cgcaccagcg cctgcgccca
     1681 cagcgccgcc actctcgcgg tgaccgggtg cagcgctccg gccagagcca gcggcccaac
     1741 cacgtagagg agcactagcg tcgccaccgc cgccagtttg agaaacagca acgtgaggag
     1801 gaggaggccc gccaccagcg caagcacgag gacgattccc ggcgcggccg ctggcagggt
     1861 ggtcagggcg agcaacttca cccagacgcc gttcacctcg tcctgcccgg cggccagtac
     1921 tccgtcgatc agcgtgttga tcccggccag gaactcgtcc atgaggagcg gatagaagcc
     1981 gatcgccgcc agcaccgcca ccagccgtcc caggagactc cccggatcgg gcaggtagcc
     2041 atagagatat tgcagcgccg ccgccgcgaa gaccgccagg cacagggcca ccgccgcatc
     2101 gcgcagcgcc aggaaaacgc tgcgcacgtt ggccagcccg ccaccgccag cgtggaggtc
     2161 ggggttatgg agcagcccgt cgaggacggg ctggccggtc gcttgcacca gtgaattgac
     2221 cagcagacgg acgaggccgc gcaggagcgc gccgaggagt tcaccccagt cggccgccgc
     2281 gagtccgccg agcgccgcgc ggacgacgct gcctacggtg gggatcggca agcggatatc
     2341 ggtgcaggcc tcgcccaggt tcgccggcag cgtcacgcag aaggaaccgg tcgccggctg
     2401 gtcggtggtc gcgctgaggc cggggtaact cccaccgtcg ctcgcgccgc cgccggcaca
     2461 gacgccgctg gcggcggtgc tgccgcaact ggtggccctg gccccgttca gaccatcgcg
     2521 cttcggcgca tccggagcac acttcggcgc cggtttcggc gtggtgtcgc cccactgcgg
     2581 cgtgaagtcc acgccccaga cctggcactg gtcgttgaag ccgttgcccg cgctgccgtc
     2641 acgcacgtcc actgcgctca ggtcgaagcc ggtcgccggg cagcccgccg gtaccttggt
     2701 ctggcaccag tccttgaccc aggcgaaggc gatgcgcgcg gcgtcggggt cgggcacgcc
     2761 gtaggagtac atctcgcggt gccacttgtc gtactgcttg cgccccccag cagtgtccag
     2821 cacggcgatg taggagacgc cgtccaccag tgcggtcccc gccaccgtgg ctgtcgggcg
     2881 cggcgctggt gtgcttgtcg ccgggtgagg cgtcggcgtg ggcgcggccg ccagcgcggc
     2941 cggcgccagc agcgcgccga gcagtgtgag cagcgtggac agtaaggcca gccgccgcaa
     3001 accgctgccg agagacatgg cgtcctccgc tcactcgccc aggtaagcac gcgggtccac
     3061 gacgcgatag cccgacgcca gcgccgggtc gacgacgcgc acctcgacgt ggacgtgggg
     3121 accattggcg ctgccgctca ctcccactgg ctggctggcg cgcacgacct ggccgacccg
     3181 caccgtaatt cggcgcaggt ggccgagaat gaccaggtcg ccattggcac gccgcagccg
     3241 cagttcgccc gaggtcgccg ggtccacgcc aggctcgtcc tcgtcacggt agtagcccga
     3301 gccgccggcg atctcgacga cgccgtccac cgggctggcg agtggcgtgc cggccggcgt
     3361 gccgacgtcg atgcccgggt gcgcgtcggc gggcaggccg taggccgcgc cgtagccgta
     3421 gttgtcacgc cctgagccgg tggagaagtc ggtgcggccg aactcctgcg tgatcgggta
     3481 ggtccgccca ccggtgatgg cccagaaacc cgcacgggct accggtgggc gggccacggc
     3541 ggcgagcagc gccaggatgc cctcggcgta ggccgcgccg caggcaaagc cgttggctgc
//
LOCUS       DASUBI010000294         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_724815, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000294 DASUBI010000000
VERSION     DASUBI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724815"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFU72_05615"
     CDS             <1..248
                     /locus_tag="VFU72_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5422998.1"
                     /translation="VLAEIPLPDELAQSVQQAPDPRVGPFARLAHDWVRSIAERDGDG
                     RAPTFADGMRVQEIVDGAMKSQELGRWIDTSGKKWPV"
     gene            261..1370
                     /locus_tag="VFU72_05620"
     CDS             261..1370
                     /locus_tag="VFU72_05620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5422999.1"
                     /translation="MSAMSYRDTLDTPCLVVHQEILDQNLAEMADYARQHGINLRPHQ
                     KTHKTAEIARMQVEAGAVGGTCAKLGEAEALVDAGALDDVLLAYQIVGPLKIARLLSL
                     MERAHVTVGVDGVDSARRLSDAVKQAGKTLDVLIEVNTGLNRAGLRPGQEVLDLAREV
                     KAMPGLRLRGIFTHEGHVARAQDGDELAGIARQAGEELVETAELLREHNIPVEVVSVG
                     STPAAFATTGVEGITEMRPGTYVFNDNSAFRLGRIGPERCALRILTTVISRPSPDRAV
                     VDAGSKTLAMDPSPGRPGHGYVVEIPDATIVRLSEEHGVIELPEDARELQVGDTLEII
                     PNHVCPTVNLQDELFVVRGDEVVACWPVIARGKVR"
     gene            1797..2570
                     /locus_tag="VFU72_05625"
     CDS             1797..2570
                     /locus_tag="VFU72_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5423000.1"
                     /translation="MRKVGSTLLAVALVLGMALVFSAGPAAAATCSQVTTLKGQMTAS
                     VIATSDQVVTGDIDATGCDIGVFVGSGVSGVTVNATVHDANQYGVFNQGNVTVDQSEI
                     SNIGNHSGGVFAPNGGQTGLGVYFAFGSNASGAVTNSVIRAYQKGGVAVNGSGASATV
                     TGNTIVGLSDVPFIAQNGIQFGYGASGVIHDNTIDGNWYTGADWSATGLLLFDVNASG
                     VKTSQNHFMNNQVNLTMVEDNACSSMYGGFYENYGLCVF"
     gene            complement(2637..3299)
                     /locus_tag="VFU72_05630"
     CDS             complement(2637..3299)
                     /locus_tag="VFU72_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423001.1"
                     /translation="MGRQPDSADEHAETVGGPGWLSIEAACRILGVDQSTLRRWSDEG
                     KVPVFRTPGGHRRYREDDLHALQRGEPRARRRMSRQVLTDLSLSGYEADYLRQAHTRR
                     WFRAYNAEQLAELRASGRNLVDLAVRSISGRGDRQHLLDESRALGRRYGALAAEAGLS
                     AEEAVEAFLFFRYPVLQAVTRFIEEENLPARRAARTFVEISQFLDQVLLATIAAHDDL
                     AP"
     gene            3484..3702
                     /gene="infA"
                     /locus_tag="VFU72_05635"
     CDS             3484..3702
                     /gene="infA"
                     /locus_tag="VFU72_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5423002.1"
                     /translation="MARSDMIQAEGAVVDALPNAMFTVELENGHKVLAHASGKMRKNF
                     IRILPGDRVRVELSPYDLERGRITYRFR"
     gene            complement(3683..>4796)
                     /locus_tag="VFU72_05640"
     CDS             complement(3683..>4796)
                     /locus_tag="VFU72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5423003.1"
                     /translation="DTIAVVARSQYLVLWSRLGRYDSAWLDELHFPDRQLFEYWAHAA
                     SIIPLELYPYFRRRMHDFASRARDYHTDWSEENGEVFEHIRERIRRQGAVSSTDFDRP
                     PGSGPVEPWSWYGGKPTNRALDILWSTGELGIQRRVNFQRHYHFAHHLYPEHHESELP
                     SADEEQRVLALTAVRAMGVVAPRWLNDYFRTRWGVRGQGGPKPEELLAGLAAAGALIP
                     VEVDAIGGAYVAATNLPLLEAVGNGARPSRTTLLSPFDNLIWDRARTQELFDFEYRIE
                     CYTPAPKRKYGYYTMPILHHGKLVGRLDPKVDRKTRVFSVRSLHLEPGVTLDESLAGA
                     LDGVLTEFAAFNGAETVDLVAAPRLAEMIAANGNGR"
BASE COUNT          796 a         1559 c         1676 g          765 t
ORIGIN      
        1 cggtgctggc cgagattccg ctgccggacg agctggcgca gtcggttcag caggcacccg
       61 acccgcgcgt cgggccgttc gcgcggctgg cccacgactg ggtgcggagc atcgctgagc
      121 gggatggcga cggacgggcg ccaaccttcg cggacggcat gcgcgtgcaa gagatcgtcg
      181 acggggcgat gaagagccag gagctgggcc gctggatcga caccagcggc aagaagtggc
      241 cggtctaggc cggggaggcg atgagcgcga tgagctaccg tgacacgctg gatacgccct
      301 gtctggtggt ccaccaggag attctggacc agaacctggc cgagatggct gactacgcgc
      361 gccagcacgg gatcaacctg cgcccgcacc agaagacgca caagacggcc gagatcgcgc
      421 gcatgcaggt ggaggctggc gccgtcggcg gcacctgcgc caagctcggc gaggccgagg
      481 cgctggtcga tgccggtgcg ctcgacgacg tgctgctcgc ctaccagatc gtgggaccgc
      541 tgaagatcgc gcgcctcttg agcctgatgg agcgggcgca cgtcaccgtc ggggtggatg
      601 gtgtcgactc ggcgcggcgg ctctccgacg ccgtgaagca agcgggtaag acactcgacg
      661 tgctgatcga ggtcaacacc ggcctcaacc gtgccgggct gcggccgggc caggaggtgc
      721 tggatctggc gcgcgaggtt aaggccatgc ccggcctgcg cctgcgcggc atcttcaccc
      781 acgagggcca cgtcgcgcgc gcccaggacg gcgacgagct ggccgggatc gcccgccagg
      841 ctggcgaaga gctggtcgag acggcggagc tgctgcgcga gcacaacatc cctgtcgagg
      901 tcgtcagcgt cggctcgacg ccggctgcct tcgccaccac cggcgtcgag gggatcaccg
      961 agatgcgccc gggaacctac gtcttcaacg acaacagcgc ctttcgcctg ggccgcatcg
     1021 gccccgagcg ctgcgccctg cgcatcctca cgaccgtgat cagccgcccc tcgcccgacc
     1081 gcgcggtggt cgacgccggt tcgaagacgc tggcgatgga cccctcaccg ggccggccag
     1141 gccacggcta cgtggtcgag atcccggacg ccaccatcgt gcggctttcg gaggagcacg
     1201 gtgtgatcga gctgcccgag gacgcgcgcg agctgcaggt gggtgacacg ctagagatca
     1261 tccccaacca cgtctgcccg actgtcaacc ttcaggacga gctgttcgtg gtcaggggcg
     1321 acgaggtcgt cgcctgctgg ccggtgatcg cccgcggcaa ggtgcgctga gggcggaacg
     1381 agccgcgcaa acactctcgg cggcagatga gccggtcgac cgtccaccgt ggcgaggctc
     1441 gcatcttgtc gttgccgagg atgcgcggcg acggacaggg cgcacgcccg agcgatagct
     1501 ttacgtttga tggggaaccc cgcccctgtc ccgcagcgct ggttgcctgg ccgtccctca
     1561 acgcgcggcg gtgaagttga cccacatcac cgtttcccgc gtcgctccga acacctatcg
     1621 cggcgatacc tcgtacagcg cttgcgattg atcgatcctc ttgacagatg aacctgacca
     1681 tcgtagtatc gtatcaggat gttcttgtgg atagcgtgag acgcagcgac agtacgcgtg
     1741 gaaggccgcg cgtagttcgt cgtgtgtcgt acactaatag tctgcggaga gggaagatgc
     1801 gcaaagttgg atcgacgctg ctggcggttg ctctggtgct gggtatggcg ctggtctttt
     1861 cggccgggcc ggctgccgcc gcgacgtgtt cgcaggtcac gacgctcaaa ggccagatga
     1921 cggcaagcgt cattgccacc tcggaccaag tcgtcactgg cgacattgac gcgacgggat
     1981 gcgacatcgg ggtcttcgtc ggctcgggcg tcagcggggt gaccgtcaac gctacggtgc
     2041 acgacgccaa ccaatacggc gtgttcaatc aaggcaacgt cacagtcgat cagtccgaga
     2101 tctccaacat cggtaatcac tccggcggcg tgttcgcccc gaacgggggg cagaccgggc
     2161 tcggcgtcta cttcgccttt ggctcgaacg ccagcggcgc ggtgaccaat agtgtcatcc
     2221 gcgcctatca gaagggcggg gtcgcggtca acggatcagg cgcgtcggcc acggtgaccg
     2281 gcaacacgat cgtcgggttg agcgacgtgc cgttcatcgc gcagaacggg attcagttcg
     2341 gctacggcgc cagcggcgtg atccacgaca acacgatcga cggcaactgg tacaccggag
     2401 cggactggtc cgcgaccggc ctgttgctct tcgacgtaaa cgccagcggg gtgaagacgt
     2461 cgcagaacca cttcatgaac aaccaggtca acctcaccat ggtcgaagac aacgcctgtt
     2521 cgtcgatgta cggcggcttc tacgagaact acgggctctg cgtattctag gcggggtccg
     2581 attcgcggcg ccgcggccca tccttcgccg ggtgggccgc tcgcgttgag caaccgtcac
     2641 ggcgccaggt cgtcgtgggc ggcgatggtg gcgaggagga cttgatccag gaactggctg
     2701 atctcgacga aggttctggc ggcgcggcgg gctggcaggt tctcttcttc gatgaagcgc
     2761 gtgaccgcct ggaggaccgg gtagcgaaag aacaggaatg cctcgaccgc ctcttcggcg
     2821 ctcagcccgg cctcggcggc cagggctcca tagcgccggc cgagcgcgcg gctctcatcg
     2881 agcaggtgct ggcggtcgcc gcgcccgctg atcgagcgca cggccagatc gaccagattg
     2941 cggccgctgg cgcgcagctc ggcgagttgc tccgcgttgt aggcgcggaa ccagcggcgc
     3001 gtatgcgcct gacgcaggta gtcggcctcg tagccggaca gcgagaggtc ggtcagcacc
     3061 tggcggctca tgcgccggcg ggcgcgcggc tcgccgcgct gcagagcgtg caggtcatct
     3121 tcgcgatagc gccggtggcc gccgggggtg cggaagaccg gcactttgcc ctcgtcgctc
     3181 cagcgacgga gggtcgattg atcgacgccg agaatgcggc aggccgcctc gatactcagc
     3241 cagcccggcc cgcccaccgt ctcggcgtgt tcgtccgcgg aatccggctg ccgacccatc
     3301 gcgattggcc tccatagcgt agaattaacg gtggacttgc tcgagtctag cgggtgtggt
     3361 tacccgtgac aacggcccgg cgcgtgcgtc agattgacgc gcgagtccag cccgcgaccc
     3421 ggccgcgagg agtgccgtgg gcgtgggcaa tgagagcagc tttacgaaag gaacacacac
     3481 catatggcca ggagcgacat gatccaggcg gagggtgcgg tcgtcgacgc gctgcccaac
     3541 gccatgttta ccgtcgaact ggagaacggc cacaaggtcc tggcgcatgc ctccggcaag
     3601 atgcgcaaga acttcatccg tattctcccc ggcgaccgcg tgcgcgtcga gctttccccg
     3661 tacgacctcg aacgcggccg tatcacctac cgtttccgtt agccgcgatc atctccgcca
     3721 gccgcggcgc agccacgaga tcgaccgtct ccgcaccgtt gaaggccgcg aactcggtca
     3781 gcacgccgtc gagcgcgccg gccagcgact cgtcgagcgt cacgcccggc tccaggtgga
     3841 gcgagcgcac cgagaagacg cgcgtcttgc ggtccacctt ggggtccagg cggccgacca
     3901 gcttgccgtg gtgcaggatc ggcatggtgt agtagccgta cttgcgcttg ggcgccgggg
     3961 tgtagcactc gatacggtac tcgaagtcga acaactcctg ggtgcgcgcc cgatcccaga
     4021 tcaggttgtc gaagggtgag agcagcgtgg tacggctggg ccgcgcgccg tttccgaccg
     4081 cctccagcag cggcaggttg gtggccgcga cgtaggcgcc gccgatcgcg tctacttcga
     4141 cggggatcaa cgcgcccgct gcggccagtc cggccagcag ttcctccggc ttcgggccac
     4201 cctggccgcg cacgccccag cgggtgcgga agtagtcgtt gagccagcgc ggcgcgacga
     4261 ctcccatggc tcgcacggcc gtcagcgcca gcacgcgttg ctcctcatcc gcgctcggca
     4321 gctcagactc gtgatgctcc ggatagaggt ggtgggcgaa gtggtagtgg cgctggaagt
     4381 tgacccggcg ctggatgccc agctcgccgg tcgaccagag gatgtcgagc gcccggttgg
     4441 tgggcttgcc gccgtaccag ctccagggct cgaccggccc gctccctggc gggcggtcga
     4501 agtcggtcga gctgaccgcg ccctgacggc ggatgcgctc ccggatatgc tcgaagacct
     4561 cgccgttctc ttcactccag tcggtgtggt agtcgcgcgc gcggctggcg aagtcgtgca
     4621 tgcgacggcg aaagtagggg tagagctcca gcgggatgat cgaggccgcg tgcgcccagt
     4681 actcgaacaa ctggcggtcg ggaaagtgca gttcgtccag ccaggcggag tcgtaccgcc
     4741 cgagccggct ccagagcacg agatactggc tgcgcgccac caccgcgatg gtgtcg
//
LOCUS       DASUBI010000295         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1083690, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000295 DASUBI010000000
VERSION     DASUBI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083690"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFU72_05645"
     CDS             complement(<1..1081)
                     /locus_tag="VFU72_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU5423004.1"
                     /translation="MIRATNARLGMLGILHVIALAGLALLPLALLPAPSTAAPATQRW
                     GYYVTYDNTSLATLQQHIGQLDVVVPYFYQLNADGTLKDTTNPTALALMRAAGVRILP
                     MIKNVAQYNDFHTSMASPAQRDAVATTITNLVLTNNYDGIHIDFEGLNPADGSLLTDF
                     MQRLAVKLHAQGKMVTQALAARTNDAAAGWAGAFDYAALGKVNDFVAIMAYDFHYAGG
                     SPGAVAPANWVQQVAAYTTTRIPAEKVILGVPFYGYDWDTTSNDNAHSVRFDQTATLL
                     AQPGVARQFDSTAQTPWFTYKDSAGHAHEVWYEDADSLHAKLQVMLDNRLAGFASWRL
                     GQEDPAAWAEIARLNTPATRIPPFTS"
     gene            complement(1303..2550)
                     /locus_tag="VFU72_05650"
     CDS             complement(1303..2550)
                     /locus_tag="VFU72_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423005.1"
                     /translation="MAQPGRRNQPPRRPRRGASRGPRSGGSSSVGIVVATTLLVIVAA
                     LVGWLAVGFRHGSSSPSPGATPSPTALALAPSPTAPVKETPVATVTATAAATAVPSPK
                     PTSTSQSAAVKTPEPTSTAAPTAAPTTPSKPATGAFGELPPADIPTGNAAGRQMSLDY
                     RLDMSLQQVPQNAAVYQLQPRTWTKDDVGALAKSLGLTGAVSDQGGAYRVSGGGDLYV
                     AGNFVQSIAGAETATPTHAPLPDDAAAVQTARSWLLRHDLVGANLGAGQVVARDEQAG
                     KVQVRIKPADPDSILSATPSATVTLGPGGQVQEADCRWPGDTQQASYGLRGADALWSD
                     AKQGQGYLEVDPNALPAGNLQGTATITGASIAYTIAGSPSGVQYLVPLVVFDGQATFQ
                     GAKAPVAIKVYVPAVSAQSAPRG"
BASE COUNT          337 a          848 c          996 g          450 t
ORIGIN      
        1 tgctggtgaa gggcgggatg cgcgtggccg gggtgttcag ccgcgcgatc tcggcccagg
       61 cagccggatc ctcctgaccg agccgccagc tggcgaagcc ggccagccgg ttgtcgagca
      121 tcacctgcag cttggcgtgc aggctgtcgg cgtcttcgta ccagacctcg tgcgcgtggc
      181 cggcgctgtc tttgtaggtg aaccacggag tctgcgccgt gctgtcgaac tggcgcgcga
      241 cgccgggctg ggccagcagc gtggccgtct gatcgaagcg caccgagtgc gcgttgtcgt
      301 tgctggtcgt atcccagtcg tagccgtaga agggcacgcc caggatgacc ttctccgcgg
      361 ggatgcgggt agtggtgtat gccgcgacct gctggaccca attggccggc gcgaccgcgc
      421 cggggctgcc gcccgcgtag tgaaagtcgt aggccatgat cgcgacgaag tcgttgacct
      481 tgccgagcgc ggcgtagtcg aaggcgcccg cccagccggc cgccgcgtcg ttggtgcgcg
      541 cggccagcgc ctgggtgacc attttgccct gcgcgtgcag cttcaccgcc aagcgctgca
      601 tgaagtcggt cagcagcgag ccgtcggcgg ggttgagccc ctcgaagtcg atgtggatgc
      661 cgtcgtagtt gttcgtgagc accagattgg tgatggtggt cgccaccgcg tcgcgctgcg
      721 cggggctggc catgctggtg tggaagtcgt tgtactgggc cacgttcttg atcatcggca
      781 ggatgcgcac gccggccgcg cgcatcagcg ccagagcggt ggggttggtc gtgtctttga
      841 gcgtgccgtc ggcgttgagc tggtagaagt agggcacgac gacgtcgagc tggccgatgt
      901 gctgctgcag ggtggcgagt gaggtgttgt cataggtgac gtagtagccc cagcgctgcg
      961 tggcgggcgc ggcggtcgag ggagcgggca gcagggccag cggcagcagc gccagcccgg
     1021 ccagcgcgat gacgtgcagg attcccagca tccccaggcg cgcgttcgtc gcgcggatca
     1081 tggcagcccg ctcccctccc caaattctga tgctcggtca tcgtcgcgtc agatcgcgct
     1141 cgtttggtca cctttggcgg gttaacgccg catcggcggc ggggtcgcgt ggggtcgtcg
     1201 catttccggt catctaggag cgtcgacgcc gttgggcgcg cggtggtttg gaccccgcgc
     1261 gctgtcgcgg cgacaaccca atgaatccgt caatctctca actcaccccc gcggcgcgga
     1321 ctgtgcgctc acagccggca cgtagacctt gatcgcgacc ggggctttgg caccctggaa
     1381 ggtcgcctgg ccgtcgaaga cgaccagcgg cacgaggtat tgcaccccgc tcggtgagcc
     1441 ggcgatggtg taggcgatgg aggcgccggt gatggtggcc gtgccctgga gattgccggc
     1501 cggcagcgcg ttgggatcga cttcgaggta gccctgaccc tgcttggcgt cagaccagag
     1561 cgcgtcggcg ccgcgcagcc cgtagctggc ctgttgggtg tcgcccggcc agcggcagtc
     1621 ggcctcctgc acctgacccc cgggtccgag ggtcaccgtg gccgagggcg tcgccgagag
     1681 aatgctgtcg gggtcggccg gcttgattcg cacctgcact ttgccggcct gctcgtcacg
     1741 ggccaccacc tgaccggctc ccaggttggc gccgaccaga tcgtgccgca ggagccagga
     1801 gcgagccgtc tggacggcgg cggcgtcgtc cggcagcggc gcgtgggtcg gagtggccgt
     1861 ctcggccccg gcgatggact gcacgaagtt gccggcgacg tagaggtcgc caccgccgga
     1921 gacgcgatag gcgccgccct gatcgctgac cgcgccggtc agccccagcg acttggccag
     1981 cgcccccacg tcgtccttgg tccacgtgcg cggctgaagc tggtagaccg ccgcgttctg
     2041 tggcacctgc tgcagactca tgtcgagccg gtagtcgagc gacatctgac gcccggcggc
     2101 attgccggtc gggatatcgg ccggcggcag ctcaccgaag gcgccggtgg ccggcttgct
     2161 cggtgtcgtc ggcgcggcgg tcggggcggc ggtgctggtc ggttcgggtg tcttcaccgc
     2221 ggcgctctga ctggtactcg tcggcttcgg gctgggcacg gcggtagcgg ccgcggtagc
     2281 ggtgacggtt gccaccggcg tctccttaac gggggcggtg gggctgggtg ccagggcaag
     2341 cgcggtcggg cttggcgtgg ctccgggtga tggtgaggag gatccgtggc ggaagccgac
     2401 ggcgagccag ccgaccagcg ccgccacgat caccagcagc gtggttgcca ccacgatgcc
     2461 gaccgaagac gagccgcccg agcgcggccc gcggctcgcg ccgcgccggg gccgtcgagg
     2521 cggttggttc ctgcgtcctg gctgtgccat gcgctccgtt gctctctagc gccggcggca
     2581 ctgggcgagc gagcggcagc cggcgccgcg gtctatctcc tgctcacggt c
//
LOCUS       DASUBI010000296         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1025006, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000296 DASUBI010000000
VERSION     DASUBI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025006"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFU72_05655"
     CDS             complement(<1..769)
                     /locus_tag="VFU72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423006.1"
                     /translation="MRDDGAETIPWRRNLYVLWIGETLTILGFSMCYPFLPFYLADLG
                     ARSFASQALWSGALLAASAGFMTITSPFWGMAADRYGRKPMLVRAMFCGALTTGLMGL
                     VVAPWQLLILFILDGALSGTVSAAMTLVASTTPKERLGYALGLLQTAVFTGISLGPLV
                     GGVLADRVGYRPVFGLGSLLLIVAALLALTLTRERFVRAPRGQADDVSDGREARLLAI
                     FLAGAVPAAIGILFALRLASAAVTPILPLFVERLSHTG"
     gene            979..1383
                     /locus_tag="VFU72_05660"
     CDS             979..1383
                     /locus_tag="VFU72_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423007.1"
                     /translation="MADARSIIPWLKSAAREHWLPLLLLIPPVVWVVYQSDAENILPM
                     LPLPLAAFLIGLVLRPRHPWLLWLGSVVFEWIVVIAWGKYDVPGSGETLTSIMLEAFF
                     WMAAGVWLPVWIGRAIRTAYGADRRSDQSPAP"
     gene            complement(1432..2166)
                     /locus_tag="VFU72_05665"
     CDS             complement(1432..2166)
                     /locus_tag="VFU72_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423008.1"
                     /translation="MLYQGAPADDGWSLAIPRLDAPPPSQRALAGLKAAIDAGEGTTP
                     IVFIEELFPNLSPALRAAGFVISLQTPLMLCSPAMLQPPPLIPGLTVSVVTGESPLAD
                     VQVALDVNEQGFDPSYSGHASIDAAEQFRQSLTAARAFIGRLDGNPVAGGMYQAPHAG
                     ITRLDGIATLEHYRRRGIGASLSATITGAAFSAGGELAFLSAANETAERLYTRIGYRR
                     AGTIVTWVEERHRRPEDGDGGHGSIP"
     gene            complement(2474..2980)
                     /locus_tag="VFU72_05670"
     CDS             complement(2474..2980)
                     /locus_tag="VFU72_05670"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5423009.1"
                     /translation="MNDGLLDAVRHNAWASGQVLQTCRALTPAQLETTVPGTYGSIID
                     TLRHMISSEASYWARLSGDEPGWDRRAEDAPGLAVLAARNDELAARWEEFLAHPFDAE
                     RTFAIRWHDGGIRDVPAGVVLMQAIHHGNDHRTQICTALTVLGIPAPELGLWDYAEAT
                     DRAPRRPA"
     gene            complement(3063..3662)
                     /locus_tag="VFU72_05675"
     CDS             complement(3063..3662)
                     /locus_tag="VFU72_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423010.1"
                     /translation="MRQDDARLLTREATLHAEALTVLDDLNLLECLAMLGQPVLVGSV
                     ALGLMTWHDIDITVLCPRLDPQATFAVMQPLAGHPRVGELRFRNDTGSWNQDPSLPDG
                     LYWGLGYRDAAGEDWKLDLWFLHETTRQPDLEHLETIPPRLTPETRLAILRIKDVWCR
                     LPSYRRTVRSYNIYQAVLDHGVRTPEDFRAYLEASGQAD"
     gene            3877..4074
                     /locus_tag="VFU72_05680"
     CDS             3877..4074
                     /locus_tag="VFU72_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423011.1"
                     /translation="MYERRLIPLRAARERLGVSKATMARLVKEGRFTIYENPLDRRQK
                     LVDEQELERTVRPRPRPAPAE"
     gene            4123..>4888
                     /locus_tag="VFU72_05685"
     CDS             4123..>4888
                     /locus_tag="VFU72_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423012.1"
                     /translation="MDPSRISLEDDRRGLSGLAFRFGNRTTYRGLEAGWHVWRVEIFV
                     APTDELQLVEVDEQGNKSSRQPAPGEMFDALELLSEPGPQTFAALAALVLTGVELRAA
                     GEAAQDELGALLRAAAEDTSAPMPLHLPGDERPLHLSQQGRQVLRQLADTLAREGRAM
                     ATLPAVRRLQVSPELIDALHKRVRRQHLQAYRTGDHWFVVLENLPKAPEDESPLLARA
                     EEPATPEHEQPSGSSNGYHRDDAGASSPSQATEEPAT"
BASE COUNT          796 a         1644 c         1704 g          744 t
ORIGIN      
        1 cgccggtgtg gctcagccgc tcgacgaaga gcggcagaat cggcgtgacg gccgcactcg
       61 ccagccgcag ggcgaagagg atgccgatcg ccgccggcac cgcgcccgcc aggaagatcg
      121 ccagcagacg tgcctcgcgc ccatcgctca cgtcgtcggc ctggcctctc ggcgcgcgca
      181 cgaagcgctc gcgcgtcagg gtgagcgcca gcagcgcggc gacgatcagt agcaggctac
      241 ccagaccgaa gaccggccgg tagccgacgc ggtcggccag caccccgccg accagcggcc
      301 cgagcgagat gccggtgaag acggcggtct gcagcagccc cagcgcgtat cccaggcgct
      361 ccttgggcgt agtcgaggcg accagcgtca tggcggccga cacggtgccg gagagcgctc
      421 cgtcgaggat gaagaggatg agcagttgcc agggagcgac gaccaggccc atcaatccgg
      481 tggtgagcgc gccgcagaac atggcgcgca ccagcatcgg cttgcgtcca tagcggtcgg
      541 cggccatacc ccagaagggc gaggtgattg tcataaaacc cgcgctggcg gccagcagcg
      601 cgccggacca gagcgcctgg ctggcgaagg agcgcgcgcc gagatcggcc aggtagaagg
      661 gcaggaaggg gtaacacatg ctgaagccga ggatggtcag cgtctcgccg atccacagca
      721 cgtagagatt gcgccgccag ggaatggtct ccgcgccgtc gtcgcgcacg ctcgacgctc
      781 ctcgatcgtc gtcaggctga catcgccgga ttgtcgcacc gcgggagcgg cggtgtccag
      841 ttgatctgtg gctcgactcc gacgtgaggc ggtgcatcca ccaaatctgt cacgggattg
      901 ttacgcaatc tcggcgcgtg atgccgcccg tacacgcgcg aggctcgata ctcggcctga
      961 gggacagagg aggatgcgat ggccgacgcc cgatcgataa tcccatggct gaagagcgcc
     1021 gcgcgcgagc actggctgcc gctgctgctg ctgattccgc cggttgtctg ggtggtctac
     1081 cagtcggacg ccgagaatat cttgccgatg ctgcccttgc cgctggcggc cttcctgatc
     1141 gggctggtgc tgcggccacg gcacccctgg ctgctctggc tgggcagcgt cgtgttcgag
     1201 tggatcgtcg tgatcgcctg gggcaagtac gatgtgccgg ggtcgggcga gacgctcacc
     1261 agcatcatgc tcgaagcgtt cttctggatg gccgcgggtg tctggctccc ggtctggatc
     1321 ggccgagcga tccgaacggc gtacggcgcg gaccggcgct ccgaccagtc gccagcgccc
     1381 taagaatacg ggcacgcgag agttcgggct tctctgctgg aggcggattg atcatggaat
     1441 actgccgtgc ccgccatcgc catcttcggg cctacgatgc cgctcctcga cccaggtcac
     1501 gatcgtgccg gcgcgccgat agccgatgcg cgtgtagagc cgctcggctg tctcgttggc
     1561 ggccgacaag aaggcgagct cgccaccggc cgagaacgcg gccccggtta tcgtcgcgct
     1621 cagtgacgcg ccgataccac ggcgccggta gtgctcgagc gtggcgatgc cgtcgagccg
     1681 ggtgatcccg gcatgaggcg cctgatacat gccgccggcg acggggttgc cgtcgagccg
     1741 cccgatgaac gcacgggcag cggtcagcga ctgccggaac tgctccgcgg cgtcgatact
     1801 cgcgtgcccg ctgtagctcg gatcaaagcc ctgctcgttg acatcgagcg cgacctggac
     1861 gtcggcgagc ggtgattcgc ccgtcacgac gctgacggtc agaccgggaa tgagcggcgg
     1921 cggctgaagc atcgcgggtg aacaaagcat caacggcgtc tgcaacgata tcacgaagcc
     1981 tgccgctcgc agcgccgggg agagattcgg gaacagctcc tcgatgaaga cgattggcgt
     2041 ggttccctcg cctgcatcga tggctgcttt caaccccgcg agcgctcgtt gcgaaggcgg
     2101 cggcgcgtca agccgcggga tcgccaggct ccagccgtcg tcggctggcg cgccctgata
     2161 gagcacgaag agcgggatga cgcgcgcttc gctactgctc tcccgcgcgg tccagtgcgc
     2221 aaactcgtgc agtcgccgca agacccctga cgtgtgcaag tcgtagcctc ccgccgtgtt
     2281 gcgttcgcag gtcacgaacg acgtcccggc actactcccg tggcagatgg tagcgcgcgc
     2341 gccctgaggc caggctgagt gggggtggcc ttgggcggcg atgcccgcga cacgtaacgc
     2401 gcataactgt ccttatgcgc acgtcatcga ttcgcccgct gctcgtgccg ggtggcgccg
     2461 cggcgtgcgg gcgctacgcc gggcgccgcg gggcgcggtc ggtagcctcg gcgtagtccc
     2521 acaggcccag ttcaggcgcc ggtatgccga ggacggtcag cgcggtgcag atctgggtgc
     2581 gatggtcgtt gccgtggtgg atggcttgca tcagcaccac gccggcgggc acgtcgcgga
     2641 tgccgccgtc gtgccagcgg atggcgaagg tgcgctcggc gtcgaacggg tgcgcgagga
     2701 actcttccca gcgcgccgcc agctcgtcgt tgcgcgcggc cagcacggcc agcccaggcg
     2761 cgtcctccgc gcggcggtcc cagcccggct cgtcgccgct gagccgagcc cagtagctgg
     2821 cctcggagct gatcatatgc cgcagcgtgt cgataatgct gccgtaggtt cccggcacgg
     2881 tcgtctcaag ctgcgccggc gtcagcgcgc ggcaggtctg gagcacctga ccgctggccc
     2941 aggcgttgtg gcgaacggcg tcgagcaagc catcgttcat gattcgcccc cgatcagcgt
     3001 gcgatacgtg agtagcgcct gaagcgtgct accggcgaga catcggccga gagcagcggg
     3061 cctcagtcgg cctggccgct cgcctcaaga tacgcgcgga agtcctccgg cgtgcgcacg
     3121 ccgtggtcga gcacggcctg gtagatattg tagctgcgca ccgtcctgcg gtacgacggc
     3181 aggcgacacc agacatcctt gatgcgcagg atcgccagtc gcgtctccgg cgtcaggcgc
     3241 ggcgggatcg tctccaggtg ctcgaggtcc ggctggcgcg tcgtctcgtg caggaaccag
     3301 aggtcgagct tccagtcctc gcccgccgcg tcgcggtagc ccagccccca gtagagccca
     3361 tccggcaggc tggggtcctg gttccaggag ccggtgtcat tgcggaagcg tagctctccc
     3421 actcgcgggt gtccggccag cggctgcatc acggcgaagg ttgcttgcgg gtcgagccgc
     3481 gggcagagaa ccgttatgtc aatgtcatgc caggtcatca gccccagcgc gacgctgccc
     3541 accagcaccg gttggcccag catggctagg cactccaaca gattcagatc gtccagcacc
     3601 gtcagcgcct cggcatgcag cgtcgcctcg cgcgtcagta gccgggcgtc gtcctgcctc
     3661 accgtcaccc ctcctctccc gggcacgctc acacgccttc tacgatgtta ccaaccagcc
     3721 ggcccggagc cgtacgcacc ggccctccgg cgggcagcga agcatccttt gcgagcgaca
     3781 cgagtcagcg aacgaacgcg cgaaccgatc ttgaccgatg cgtacggaca tgatatcgtg
     3841 tctgtgaggc aatcaccgat cgagaggagt cgcttcgtgt acgaacggcg gctgatacct
     3901 ctgcgagccg cgcgcgagcg gttgggcgtg agtaaggcga ccatggcgcg gctggtcaaa
     3961 gagggccgct tcacgatcta cgagaatccg ctcgatcgac gccagaagct ggtggacgag
     4021 caggagttgg agcgcaccgt tcgccctcgg ccccgcccgg cgcccgccga gtgagccgtg
     4081 cgtcgcacac caatcacggt gatcacgagg aggagcagcg tcatggatcc ctcgcgcatc
     4141 agtctggaag acgaccgccg tggcctcagc ggcctggcct tccgcttcgg caaccggacg
     4201 acgtaccgcg gccttgaagc cggctggcac gtctggcgcg tggagatctt cgtcgcgccc
     4261 accgacgaat tacagctcgt cgaggtggac gagcaaggca acaagagctc gcgtcagccg
     4321 gcgccgggcg agatgtttga cgcgctggag ctcctcagcg aaccgggtcc acagacgttc
     4381 gcggcgctcg cggcgctggt tctgaccgga gtcgagctgc gcgcggccgg cgaagccgcg
     4441 caagacgaac tgggcgcgct gctgcgcgcg gccgccgagg ataccagcgc gccgatgccg
     4501 ctgcacctcc ccggcgatga gcggcccttg cacctgtcgc agcagggccg gcaagtcttg
     4561 cgccagctcg ccgatacact ggcgcgcgag ggcagggcca tggcgacgct tccggcggtc
     4621 aggcggctgc aggtcagtcc cgagctgatc gatgcgctgc acaagcgggt gcggcgccag
     4681 cacctgcagg cctaccgcac cggtgaccac tggttcgtag tgttggagaa cctccccaag
     4741 gcccctgagg acgagtcgcc gctgctggcg cgcgcggagg agccagccac gccggagcat
     4801 gagcagccgt ccggatcgtc caatggctac caccgcgacg acgccggggc atccagcccg
     4861 tcgcaggcca ccgaggagcc ggcgaccg
//
LOCUS       DASUBI010000297         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_697276, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000297 DASUBI010000000
VERSION     DASUBI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697276"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFU72_05690"
     CDS             <1..240
                     /locus_tag="VFU72_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423013.1"
                     /translation="ASHRRQCSAPSVIAVLRRVPEKRLRVFALAEQLRGPAGELDLDA
                     VGRHGPAIDLACLEAEGYARGTCAAVAALRRLPAR"
     gene            250..1269
                     /locus_tag="VFU72_05695"
     CDS             250..1269
                     /locus_tag="VFU72_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423014.1"
                     /translation="MTNDHPVDQAPPPAPAAAPRLVRPVALGAALHRAPPLPTSLAHL
                     LDGHHGAPAFRQIVEAIFPAQAPAILAATQPGLDRREARVAAFLTRVEAEHFPVYTLD
                     DWYAGDDDPYDFVLGGIPFVRLGWSDEDYHELDRRDGYRLLAALCQHPYDDTGAPLAL
                     LDSLAALAPPETLRRLPQAGFPPETLHARLDGGPFAAAADFADWRWATTGTAFLDCDD
                     TMDIVDAHWTPENVALLTTQWRQADAILERIDALATWLEIDPAIHFAALLDAAIREVA
                     AEAATAGEAAAPAHAGGGPLVAALPVDAGTDVNSDGGDHGHNPTDATRDSTPGEQPVP
                     ARRAA"
     gene            1259..1936
                     /locus_tag="VFU72_05700"
     CDS             1259..1936
                     /locus_tag="VFU72_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423015.1"
                     /translation="MPPELDVEEDPLRLRLDFHDESVILHDYSDRGGVTTRLVAALEV
                     AHALASELDLTTGLLPPDTLWVVKRASGAWLALWEPPQVRTVRLRTSLEAPPRRYRLP
                     CPGLVFLCPPAGQAPYVFAARARPRREADALAHAPFYNVFASGRVCVGTHPFPHDPAR
                     VPHAFFTSYFSAVMDTARDKSRRHPDDVGRPWDDVDKLREFPLDDLVLHLTVGDAMRL
                     GAAGSHS"
     gene            1933..2637
                     /locus_tag="VFU72_05705"
     CDS             1933..2637
                     /locus_tag="VFU72_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEU5423016.1"
                     /translation="MSAPGTAPAPPLVDYLVARAGAPSPRGLAYDYVLAGDGLWLTAA
                     NAALTVRVPVARCTVRGLPPVGSACILRHGRIPGWIWAACVAITQLWATMGVEILLLV
                     TCDVAGRYALVIPEQAASTARVRYTPPMLPVGTSIVLALHSHHQLPAYFSGTDDGDEQ
                     SLALYAVVGRLDTARPEVALRAGVYGAWLPVPWADVFAGERGAFRDVQFDPLAEAETE
                     QARTVGVGDDAEEARR"
     gene            2634..3533
                     /locus_tag="VFU72_05710"
     CDS             2634..3533
                     /locus_tag="VFU72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU5423017.1"
                     /translation="MSHVVDTRPCGYDGQPLRCERAATIVLVGCGGTGSFLADALCRL
                     LIGWDARVYLIDMDRVEPHNVGRQAFDRREVGRFKAEVLAERLAQRYGRAIDYAVAPY
                     DGGTHAGSFADGTPSALNLLVSAVDNAAARRALAATIEERDPYGGWGALSRHIWLLDC
                     GNARNSGQVLLGNTMRPEALRGAFLRREGRCRALPAPGLQRPDLLVSPPEPERSLHLD
                     CAEAIAQELQGPTINQVVAAIAASMVEKLLAGTCAWMGVYFDLDAGTLRPVPAEPATV
                     AAIAGLSHNAVVPPSGRNAPAVA"
     gene            3704..3991
                     /locus_tag="VFU72_05715"
     CDS             3704..3991
                     /locus_tag="VFU72_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423018.1"
                     /translation="MTGTVNERLLLTVEEAARRLGIGRTLAWRLVRSGDLPSIRLGRL
                     VRVPQSALLVWLDRRSNGTGRQRPGNGDAAERRTRHATASGSNGKGRDGRA"
     gene            3978..>5047
                     /locus_tag="VFU72_05720"
     CDS             3978..>5047
                     /locus_tag="VFU72_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5423019.1"
                     /translation="MAEHKRRRRRANGEGSVYQRGDRGTWVAQVPVGINPDGSYRFKR
                     YSCETQAAAKAKLRAAQEALDRGADLGAKSQTLGTFLDAWLAEVVRRDAEPKTYEGYA
                     YTAGLIKARLGRVPLDKLSPQQVQKLLNELLEQGGAGGTGLSPRTVQYARATLRRALG
                     QAQKWGLVTRNVATLVEPPKSRRAEVQPFTEDEARALLAAAQGDRLEALYAVALTLGL
                     RQGELLGLRWEDLDLDRGVLRVRQQLQHMKREQPTLKGLKTRASRRDLELPARLVLHL
                     RAHRARQRRERERKGDAWREHGLVFPSAVGTPLTPRNLVRRFQELLRRAGLPARRFHD
                     LRHTAASLMFERGLEATTVQRVL"
BASE COUNT          714 a         1845 c         1778 g          710 t
ORIGIN      
        1 gcctcgcacc gtcgccagtg ctccgccccg agcgtcatcg ctgtcctgcg ccgcgtgccg
       61 gagaagcgcc tgcgcgtctt cgccctggcc gagcagctgc gcggaccggc tggcgaactg
      121 gatctcgacg cggtaggacg acacgggcca gcgatcgacc tggcctgcct ggaggcggaa
      181 ggctatgcgc gcggcacctg cgcggcggtc gcggcgctcc gacgactccc cgcccgctga
      241 agcgcaggca tgacgaacga ccatccggtt gaccaggctc cgccgccagc gcccgccgca
      301 gcgccgcgcc tggtccgccc cgtcgccctc ggcgcggcgc tccaccgcgc gccgccgctc
      361 ccgaccagcc tggcgcacct actcgatggg catcacggcg cgcccgcgtt ccgccagatc
      421 gtggaagcga tcttccccgc acaggcgccg gccatcctgg cggccacgca accggggctc
      481 gaccggcgcg aggcgcgcgt cgccgctttc ctcacccgcg tcgaggccga gcacttcccg
      541 gtctacacgc tcgacgactg gtatgccggg gacgacgacc cctacgactt tgtcctcggc
      601 ggcatcccct tcgtccggct gggctggagt gacgaggact atcacgaact cgaccgccgc
      661 gatggctacc gcctcctcgc cgcgctctgc cagcacccgt atgatgacac tggcgcaccc
      721 ctcgcgctcc tggactcgct cgcagcgttg gcgccgccag agacgctgcg ccggctcccg
      781 caggcgggct tcccgcctga aaccctgcac gcccgcctcg acggcggacc attcgccgcg
      841 gcggcggact ttgccgactg gcgctgggcg accaccggca ccgccttcct cgactgcgac
      901 gacacaatgg acatcgtcga cgctcactgg accccggaga atgtcgcgct gctcaccacg
      961 cagtggcgcc aggccgacgc catcctcgaa cggatcgatg ccctggcgac ctggctagag
     1021 atcgatccag ccatccactt cgccgccctg ttggacgctg caattcgtga ggtggcggcg
     1081 gaagcggcca cggccggcga agcggcggcg ccagcgcatg ctggtggcgg tccgctggtg
     1141 gcggcgctgc cggtggacgc tggtactgac gtgaactctg acggaggtga ccatggccac
     1201 aacccgaccg acgcgacacg cgacagcacg cccggcgagc agcccgtacc agcgcgccgt
     1261 gccgcctgaa ctcgatgtcg aggaggatcc gctgcgcctg cggctcgact tccacgatga
     1321 gagcgtcatc ctgcatgact acagtgaccg cggcggggtg acgacgcggc tggtggcggc
     1381 gctggaggtg gcgcacgcgc tggcgagcga actcgacctg acgactgggc tcctaccgcc
     1441 cgacactctc tgggtggtca agcgggcgag cggcgcctgg ctggccctgt gggagccgcc
     1501 acaggtccgt acagtgcgtc tccgcaccag cctggaggcg ccaccccggc gctaccgtct
     1561 cccctgcccc ggtctcgtct tcctctgccc acccgctggt caggcgccct atgtcttcgc
     1621 ggcgcgggcg cgcccccggc gcgaggccga cgcgctcgct catgccccgt tctacaatgt
     1681 cttcgcgagc gggcgcgtct gtgttggcac gcatcccttc ccccacgacc cggcgcgggt
     1741 gccgcatgcc tttttcacca gctacttctc ggcggtcatg gacaccgcac gggacaaatc
     1801 acggcgacac ccggatgacg tcggccgccc ctgggacgac gtggacaaat tacgcgagtt
     1861 tccgctcgac gacctcgtgc tccacctcac agtaggcgac gcgatgcgcc tcggcgccgc
     1921 cgggagccac tcgtgagcgc gcccggcacc gcccccgccc caccgctggt ggattacctg
     1981 gttgcccgag caggcgcgcc atcgccacgc ggcctggcct acgactacgt cctcgccggg
     2041 gacggcctct ggctcaccgc cgcgaacgcc gcgctcactg tccgcgtgcc ggttgcccgc
     2101 tgcaccgtgc gtggcctgcc acccgttggc agcgcctgca tcttgcggca cgggcggatt
     2161 ccgggctgga tctgggcggc ctgcgtagcg atcactcaac tctgggccac catgggcgtg
     2221 gagatcctgc tgctggtgac ctgtgatgtg gcggggcgct acgccctcgt catccccgag
     2281 caggcggcgt caacggcacg cgtgcggtac acgccgccaa tgctccccgt tggcaccagc
     2341 atcgtgctcg cgctgcattc gcatcaccag ctccccgcgt acttcagcgg caccgacgac
     2401 ggtgacgagc agagcctcgc gctctacgcc gtcgtcgggc ggctcgacac cgcgcgcccg
     2461 gaggtcgcgc tccgggctgg cgtctacggc gcctggctgc cggtgccttg ggcagacgtc
     2521 ttcgctggcg agcggggcgc cttccgggac gtacagttcg acccactcgc tgaggcggag
     2581 acggagcagg cccgcaccgt cggtgtcggc gacgacgcgg aggaggcgcg ccgatgagcc
     2641 acgtcgtcga cacgcggccg tgcggctatg acggccagcc cctgcgctgc gagcgcgcgg
     2701 caaccatcgt cctggttggc tgtggcggca ccggcagttt tctcgccgac gccctctgcc
     2761 ggctcctcat cggatgggat gcgcgggtgt acctgatcga tatggaccgc gttgagccgc
     2821 acaacgtcgg ccggcaggcg ttcgaccggc gcgaggtcgg gcgcttcaag gccgaggtgc
     2881 tggccgagcg gctagcgcag cgctatggcc gcgcgatcga ctatgcggtc gcgccgtatg
     2941 acggcgggac gcacgccggc agcttcgccg acgggacgcc gagcgccctc aatctcctgg
     3001 tgagtgcagt agacaacgcc gccgcgcgcc gggcgctggc tgcgactatc gaggagcgcg
     3061 acccctacgg cggctggggc gcgctgtccc ggcatatctg gctgctggac tgtggcaacg
     3121 cgcgcaacag cggccaggtg ctgctgggca acaccatgcg tccggaggcg ctccgcggcg
     3181 cgttcttgcg ccgcgaggga cgctgtcgcg cgctgccggc acctggcctc cagcgccctg
     3241 acttgctggt cagtccgccc gagcctgagc ggtcgctcca cctcgactgc gccgaagcga
     3301 tcgcccagga gctccaggga ccgacgatca accaggtcgt cgccgccatc gccgcgagta
     3361 tggtcgaaaa actcctcgct ggcacctgcg cctggatggg tgtctacttc gacctggacg
     3421 ccggcacgct gcgcccggta cccgccgagc cagcgaccgt ggccgccatc gctggactct
     3481 cccacaacgc ggtggttccg cccagcggtc gcaacgcgcc cgctgttgcc tgacgcggct
     3541 gtgccaaccg tccccgctgt gtcgagcacc gtccaagccg gctgtgagag cgttgtgaga
     3601 gccacgctct ctaggctggg aactacgcgc gccatggcgc gtctggtggc ggcatcggcc
     3661 aaccgcccgc ggtcgacgat agcacgatgg gaggtcacga cccatgacag gaacagtcaa
     3721 tgaacgcttg ctactgaccg tcgaagaagc agcgcgacga ctggggatcg gcaggacgct
     3781 cgcctggcgg ctggtgcgga gcggcgacct accgagcatc cggttggggc ggctggtgcg
     3841 cgtgccccag agcgcactcc tggtctggct cgaccggcgc agcaacggca ctggccggca
     3901 acggcccggg aacggcgacg cggcggagcg acgaacccgc cacgccaccg cttccggtag
     3961 caatgggaag gggcgagatg gccgagcata agcggcgtcg ccggcgcgcg aatggtgaag
     4021 gatcggtcta ccagcgcggc gaccggggta cctgggtggc ccaggttccg gtcgggatca
     4081 acccggacgg cagctaccgc ttcaagcgct attcgtgcga gacgcaggcc gcagcgaagg
     4141 cgaagctgcg cgcggcgcag gaggcgctcg accgcggggc cgacctcggg gcgaagtccc
     4201 agacgctcgg gacgttcctc gacgcgtggc tggccgaggt ggtgcggcgc gacgccgagc
     4261 cgaagaccta cgaggggtac gcctacaccg ccgggctgat caaggcgcgg ctcgggcggg
     4321 tgccgctcga caagctgtcg ccgcagcaag tgcaaaagct gctcaacgag ctcctggagc
     4381 agggcggcgc gggcggcacg ggcttgtcgc cgcgcacggt gcagtacgcg cgcgcgacgc
     4441 tgcggcgcgc cctcggccag gcgcagaagt gggggctggt gacgcgcaac gtcgccacgc
     4501 tggtcgagcc gccgaagtcg cggcgtgccg aggtgcagcc cttcactgag gacgaggcgc
     4561 gcgcgctgct cgccgccgcg cagggcgacc gactggaggc gctctacgcc gtggcgctca
     4621 cgctcggact gcggcagggc gagctgctgg ggctgcgctg ggaggacctc gacctcgacc
     4681 ggggcgtgct gcgcgtccgg cagcagctgc agcacatgaa gcgcgagcag cccaccctca
     4741 aggggctgaa gacgcgggcg agccggcgcg acctcgagct acccgcccgg ctggtgctgc
     4801 acctgcgggc gcaccgggcg cggcagcggc gcgagcggga gcgcaagggc gacgcctggc
     4861 gcgagcatgg gctggtcttc ccatcggcgg tgggcacgcc gctgaccccg cgcaacctgg
     4921 tgcggcgctt ccaggaactg ctccggcggg cggggctgcc tgcgcggcgc ttccacgacc
     4981 tgcggcacac cgccgcatcg ctgatgttcg agcgcgggct ggaggcgacg acggtgcagc
     5041 gggtgct
//
LOCUS       DASUBI010000298         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_779160, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000298 DASUBI010000000
VERSION     DASUBI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779160"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2083)
                     /locus_tag="VFU72_05725"
     CDS             complement(<1..2083)
                     /locus_tag="VFU72_05725"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU5423020.1"
                     /translation="MPRSAHRAELRARANHRFTYGGRALLISDVFGVVQGDPEGFYFE
                     NTRLLCRDRLLADVQPLFPFVASPVDGSAFLAYAEVPESDSIPPRSILLTLRRFVEDG
                     LREELIIESFHRSAPLRFELALELAADFADLAEAMSGKREQTAPVETTWDASQRELCF
                     RYRHPRLDRAVAVRVVAAPAGFAWDEARGAFAAALELEHRRPVTITLSVEPVFDGRRR
                     VPPARRFGEVVSPLDRLRRELSENIPRLTSSNASVVRTWETATADLAASPLGVASGPA
                     AASAGLPMYQQLFGRDSLTIGWQAALALPLLLRGALRMNAAWQGTVIDDWRDEEPGKL
                     LHQARNGPLSALGDNPFSAYYGDYATPPDFIAMLGQYFLWTNELAEVRALLPAARATL
                     QWLDRYGDRDGDGFLEYVTRSPDGLANQGWKDSWNAIVYPDGGIVSDPIATCEIQGYW
                     YGGLQQLAFVFAAAGDEAYAVELWRQAARLKRRFNEAFWMEDEGFYAMALDADKRQVR
                     SLASNPSQLLVTGIVPRERGPRVVRRLMQPDLFSGWGIRTLSSEHPSFNPFSYHLGSV
                     WPVDSGTAALGFARYGCVEELHRLAEGLFAAADLFDANRLPEVLGGVPRDAAHPHPGI
                     YPESNQPQGWSDSGVVLTIQALLGLRPAAPIGLLLIDPVLPDWLPELQLRGIRVGQAR
                     LDLRAWRDGRRT"
     gene            complement(2110..3120)
                     /locus_tag="VFU72_05730"
     CDS             complement(2110..3120)
                     /locus_tag="VFU72_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5423021.1"
                     /translation="MPKPLAEQVVVITGASSGIGRETAIELAMRRASLVLAARNQDAL
                     EEVSREVERLGGTALPVVTDVSDWTQLEHLAQQAVARFGRIDTWINDAAVIEYATVAD
                     TTPDESARIVRVNLLGQIDGSMAALPELTRQGQGMLVNVSSQVAIRSVPFLAVYSATK
                     HGIKGFTEALRLELAHTHPGITVTLILPGSINTPFFTHARSKLGSLPGAIPPVYEPNV
                     VAQAIVYALEHPRRDVYVGGAGRLLPLAEALAPGLLDRLMLADGLFFRLQRSDRPDNG
                     RDNLFASPAGTGATTGPWGQRSVSTSLTTRALWLHPRRLGLAMGGTLLGLLALLRWGG
                     RG"
     gene            3343..>5669
                     /locus_tag="VFU72_05735"
     CDS             3343..>5669
                     /locus_tag="VFU72_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5423022.1"
                     /translation="MPNHFPLPSDRDPAPLVGREHERALLDERLRATLDGQGSLVLVS
                     GEAGIGKTTLVANLARQAEAAGALVLWGHCYDLSITPPYGPWLELAGRYQPCDGLPPA
                     PAFLHDPAALAAAGSQEALFAQTWAFFAALAAARPLVLVLEDLHWADQESLTLLRLVA
                     RRLAGHRVLVLATYRSDDLSRRHALYPLIPLLVREAQAARLELQRLTDADRRALITQR
                     YGLPETDATRLVAYLRVHAEGNPLYAGELLRTLEETRVLVRAEHDWRLGDLGPVRVPP
                     LLLQVVEGRLSRLDDETRRLLQVAAIIGQEVPLALWQRVSEACDAALSAAIEQGQEAA
                     LLEGSPQVAGYRFRHALLRAALYEEQTIPLRRRRHRRVAELLAAARTPDADAVARHFQ
                     QAGDVRAVAWLLESARRARMTFATATALDRLETAIALDEQHDDASGLRGWLLAALASW
                     STHLPDVEQHVRMFEEASDIASRTNDEALAGLVEWSRAYLESQWFASVTEQMGRARAR
                     IHQLPAAERARLFGFLYGAVGSALDPGGPDLASVVIGFQGQSGQYRETLAAVERIHAD
                     YPVLPPVAEMNIDNALMASYQALGRPEAALQRYDRMLAAHGRDHITNWAAVVLWLKLR
                     DLVLVYWPDRIDMRRALADETVAAVRRAKSDQTFGPDMPDEIGIIWLLLVEGRWDEVR
                     RVARQATAGGRYAAFLAAAPSMILSREQGDPEAAQAGLSLVFPEGPASEPGRTTFETS
                     VACMHAAIEIALDAGDLQLARRWLECHERWIGWSGS"
BASE COUNT          841 a         1935 c         2103 g          790 t
ORIGIN      
        1 gggtacgccg gccgtcgcgc caggcgcgga ggtcgaggcg cgcctggccg acccggatac
       61 cgcgcagctg cagctcgggg agccagtcgg gcaggacggg atcaatcagc agcagcccga
      121 ttggggcggc ggggcgcagc ccgagcagcg cctggatggt cagcaccacg ccgctgtcgg
      181 accagccctg cggctggttg ctctcggggt agatgccggg gtgggggtgc gcggcgtcgc
      241 ggggcacgcc acccagcacc tccggcagcc ggttggcgtc gaagaggtcg gcggcggcga
      301 agagcccctc ggccagacgg tgcagctcct cgacgcagcc gtagcgcgcg aagccgagcg
      361 cggcggtgcc gctgtcgacc ggccagaccg agccgaggtg gtagctgaag gggttgaaag
      421 aggggtgctc gctggagagg gtgcggatgc cccagccgct gaagaggtcg ggctgcatca
      481 gccgccggac cacgcggggg ccgcgctccc ggggcacgat gccggtcacc aggagctggc
      541 tggggttgga ggcgagcgag cgcacctggc gcttgtcggc gtcgagcgcc atggcgtaga
      601 agccctcgtc ctccatccag aaggcctcgt tgaagcggcg cttgagccgc gccgcctggc
      661 gccagagctc gaccgcgtag gcctcgtcgc cggcggccgc gaagacgaag gccagctgct
      721 gcagaccgcc gtaccagtag ccctggatct cgcaggtggc gatcgggtcg gacacgatgc
      781 cgccgtcggg gtagacgatg gcgttccagg agtccttcca gccctggttg gcgagcccgt
      841 ccggggagcg ggtgacgtac tccaggaagc cgtcgccgtc gcggtcgccg tagcggtcca
      901 gccactgcag cgtggcgcgg gcggcaggta acagcgcgcg cacctccgcc agttcgttgg
      961 tccacaggaa gtactgcccg agcatggcga tgaagtcggg cggggtggcg tagtcgccgt
     1021 agtaggcgga gaacgggttg tcgccgaggg ccgagagagg gccgttgcgc gcctgatgca
     1081 ggagcttgcc cggctcctcg tcgcgccagt cgtcgatcac cgtcccctgc caggccgcgt
     1141 tcatgcgcag cgcgccgcgc aggaggagcg gcagcgccag ggccgcctgc cagccgatcg
     1201 tcagcgagtc gcgcccgaag agctgctggt acatcggcag gccggcgctg gcggcggccg
     1261 ggccagatgc gacccccagc ggcgaggcgg cgagatccgc ggtcgcggtc tcccaagtgc
     1321 gcaccacgct ggcgttcgac gaggtcaggc gcgggatgtt ctcggaaagc tcccggcgca
     1381 ggcggtcgag cgggctgacg acctcgccga accggcgcgc gggcggcacc cggcgccggc
     1441 cgtcgaagac cggctcgacc gagagggtga tcgtcacggg ccgccggtgc tccagctcca
     1501 gcgccgccgc gaaggcgccg cgtgcctcat cccaggcgaa gccggcaggc gcggcgacga
     1561 cgcgcaccgc cacggcgcgg tcgagcctgg ggtgccggta gcggaagcac agctctcgct
     1621 ggctggcatc ccaggtggtc tcgaccgggg cggtctgctc tctcttaccc gacatggcct
     1681 cggcgaggtc ggcgaagtcg gcggccagct ccagcgccag ctcgaaccgc agcggcgccg
     1741 agcgatggaa gctctcgatg atgagctctt cgcgcagccc gtcctccacg aagcgccgca
     1801 gcgtcagcag gatcgagcgt ggggggatgg agtcgctctc gggcacctcg gcgtaggcca
     1861 ggaaggcgct tccgtcgacg gggctggcga cgaaggggaa gagcggctga acgtcggcaa
     1921 ggagacggtc gcggcagagg aggcgcgtgt tctcgaagta gaagccctcg ggatcgccct
     1981 gaacgacgcc gaagacgtcg ctgatcagca gcgcgcgccc gccgtaggtg aagcggtggt
     2041 tggcccgggc gcgcagttcg gcgcgatgcg cgctgcgagg cactctgcgt ggcgccctgc
     2101 gcggggccat cagccgcgcc cgccccagcg cagcagcgcc agcaggccga gcagcgtgcc
     2161 gcccatcgcc agccccagcc ggcgcgggtg cagccagagg gcgcgcgtgg tgaggctagt
     2221 ggagaccgag cgctggcccc agggaccggt ggtcgcgccg gtgccagccg gagacgcgaa
     2281 gaggttgtcg cggccgttgt caggccgatc cgagcgctgc aggcggaaga agaggccgtc
     2341 ggccagcatc aggcggtcga gcagccccgg cgccagcgcc tcggccagcg gcaagagccg
     2401 gccagcaccg ccgacgtaga cgtcgcgccg ggggtgctcc agggcgtaga cgatggcctg
     2461 ggcgaccacg ttgggctcgt agaccggcgg gatcgcgccc ggcagcgagc cgagcttgga
     2521 gcgcgcgtgc gtgaagaagg gcgtgttgat cgagcccggc agaatcagcg tgacggtgat
     2581 gccggggtgc gtgtgggcca gctccaggcg cagcgcctcg gtgaagccct tgatgccgtg
     2641 cttggtggcc gagtagaccg ccaggaacgg tacggagcga atcgccacct gcgaggagac
     2701 gttgacgagc atcccctggc cctggcgagt cagctccggc agcgccgcca tgctgccgtc
     2761 gatctggccg agcaagttga cccgcacgat gcgcgcggac tcgtcgggcg tcgtgtcagc
     2821 cacggtggcg tactcgatca ccgccgcgtc gttgatccag gtgtcgatgc gcccgaagcg
     2881 cgccaccgcc tgctgggcca ggtgctccag ctgggtccag tcggagacgt cggtgaccac
     2941 cggcagcgct gtgccaccca agcgctcgac ctcgcggctg acttcctcaa gcgcgtcctg
     3001 gttgcgcgcg gccagcacga gcgaggcgcg ccgcattgcc agctcgatgg ccgtctcacg
     3061 cccgatgccc gaggatgccc cagtgatgac gaccacttgc tcggccagcg gcttcggcat
     3121 ggctcgtcct ccattcgctc gacactggtt tggaggtctg caaagcgggt gccggccaat
     3181 ccggatcgct attgaacggt acgggggggc aagcgcttca gattcctcaa cccgactcaa
     3241 cagcggaatc catgccggaa gagaatcggt gctacactca ggctgtcacg cgaatccgac
     3301 ggtgaacatg cacaaggact ttgccagcaa ggggcggcag cgatgccaaa ccatttcccg
     3361 ctcccgagcg accgtgaccc cgcgccgctg gtcggccggg agcacgagcg tgcgctgctc
     3421 gacgagcggc tacgggccac actggacggg caggggagtc tggtgctggt cagcggcgag
     3481 gcgggcatcg gcaagacgac gctggtggcg aatctcgccc gccaggccga agccgcgggc
     3541 gcgctcgtcc tctggggcca ttgctacgac ctctcgatca cgccacccta cggcccctgg
     3601 ctggagctgg ccggccgcta ccagccgtgc gacggcctgc cacccgcacc cgccttcctg
     3661 cacgatccgg ccgcactggc cgccgccggc agccaggagg cgctcttcgc ccagacctgg
     3721 gccttcttcg cggcgctcgc cgcggcgcgg cccctcgtgc tggtgctgga ggacctgcac
     3781 tgggcggacc aggagagcct gaccttgctg cgactggtag cccgccggct cgccgggcac
     3841 cgggtgctcg tgctggccac ctaccgcagc gacgacctca gccgccggca tgcgctctac
     3901 ccactcatcc cgttgctcgt gcgcgaggcg caggccgccc gcttggagct ccagcgactg
     3961 accgacgccg accgccgtgc cctgatcacc cagcgctacg gcttgccgga aaccgatgcc
     4021 acccgtctgg tggcgtacct gcgcgtacac gccgagggga acccgctgta cgctggcgaa
     4081 ctcctgcgca cgctggagga gacgcgggtg ctggtgcggg cggagcacga ctggcgcctg
     4141 ggcgacctgg ggccggtgcg ggtgccgccg ctgctgttgc aggtcgtcga gggccgcctg
     4201 agccggctcg acgacgagac gcgccgtctg ctccaggtgg cggcgatcat cgggcaggag
     4261 gtgccgctgg cgctctggca gcgggtgagc gaggcgtgcg acgcggcact gtcggcggcc
     4321 atcgagcagg gacaggaggc ggcgctgctg gagggctcgc cccaagtggc tggctatcgc
     4381 ttccgtcacg ccctgctgcg agccgcgctc tacgaggagc agaccatccc gctccgccgt
     4441 cgccggcatc gcagggtggc tgagttgctg gccgcggcgc gcacgcccga tgcggacgcc
     4501 gtcgcccggc acttccagca ggccggtgac gttcgcgccg tcgcctggct gctggagtcg
     4561 gctcgacgtg cgcggatgac cttcgccacg gccaccgccc tcgatcgcct ggagacggcc
     4621 atcgcgctgg acgagcagca tgacgacgcc agcggcctgc gcggctggct gctggccgcc
     4681 ctcgcgagct ggagcaccca tctgccggac gtcgagcagc acgtgcgcat gttcgaggag
     4741 gcgtcggaca tcgcgtcgcg aacgaacgac gaggcgctcg ccgggctggt cgagtggtca
     4801 cgcgcgtacc tcgaaagcca atggttcgcc tcagtcaccg aacagatggg ccgggcgcga
     4861 gctcggattc accaactgcc agccgctgag cgcgcgcgac tgttcggctt cctctacggc
     4921 gcggttggct cggcgctcga tccgggtggc cccgacctgg cgagcgtggt gattggcttt
     4981 caggggcaat ctggacagta ccgcgagaca ctggctgccg ttgagcgcat ccacgcggac
     5041 tatccggtac tgcccccggt cgccgagatg aatatcgaca atgccctcat ggcatcgtat
     5101 caggcgcttg gacggccgga agcggcgctc cagcgctacg atcgcatgct cgccgcgcac
     5161 ggccgcgacc acatcaccaa ctgggccgcc gtcgtgctgt ggctcaagct gcgcgacctc
     5221 gtcctggtct actggccgga ccggatcgac atgcgacgcg cgctggcaga cgagacggtc
     5281 gcggcggtcc ggcgcgccaa atccgatcag accttcggcc cggacatgcc ggacgagatc
     5341 ggcatcatct ggctgctcct cgtggagggc cgctgggatg aggtgcgacg cgtggcgcgg
     5401 caggccaccg ctggcgggcg ctacgccgcg ttcttggccg ccgcgccctc gatgatcctg
     5461 tcgcgcgagc aaggcgaccc cgaggccgcg caggcgggat tgtcattggt cttccccgag
     5521 ggtccggcat ccgaacccgg acgcacgact tttgaaacgt cggtggcctg catgcatgcc
     5581 gcgatcgaga tcgcgctcga tgccggtgat ctccagttgg cgcgtcgatg gctggagtgt
     5641 cacgaacgct ggatcggctg gagcggttc
//
LOCUS       DASUBI010000299         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_192988, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000299 DASUBI010000000
VERSION     DASUBI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192988"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..892)
                     /locus_tag="VFU72_05740"
     CDS             complement(11..892)
                     /locus_tag="VFU72_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU5423023.1"
                     /translation="MSDRSWGGQSMVAPLRRVLVYPPVPPAPEVSWESFGYLRPIDHA
                     LAVREHAALRRILQAAGAEVIAGEIDDAGLQDAIFPFDPLLTTDRGVILLRPGKELRL
                     GEVELGAQTLAELGVPVAGRIEAPGTLEGGDTFWLDEHTLIVGRGYRSNDAGIDQLRA
                     LLAEQGVEVLAYDLPHWHGRGEVLHLLSLISPVDERLAVVYLPLMPSRLVELLEARGW
                     GFVEVPDAEFASQGPNVLALAPRRCLLLRENTVTAERLRAAGCEVTLFAGDEISHNRT
                     GGPTCLTRPLLRQMPTS"
     gene            complement(892..1548)
                     /locus_tag="VFU72_05745"
     CDS             complement(892..1548)
                     /locus_tag="VFU72_05745"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU5423024.1"
                     /translation="MSSPPADERVTTSLRERKKARTRAEIQRQALRLFREQGFAETTV
                     EQIAAAAEVSPSTFFRYFPTKEDVVLRDDYDELLLEAFAAQPAELNPIQALRAAMRAT
                     AGRMTPEELEQEARRQRLVLATPELYPRMLSESTRTIQTLTAAIARRTGRSPDDFAAQ
                     VFTGAVIGVALSAWLAATESEADFWPLMDRALAYLEAGLPLAAEPGGGDASNTEQEAG
                     "
     gene            complement(1526..3082)
                     /locus_tag="VFU72_05750"
     CDS             complement(1526..3082)
                     /locus_tag="VFU72_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423025.1"
                     /translation="MRTADRRKWWALGALVLAVLAVGLDGTILSVALPTLAAALHASE
                     ADLQWFTSGYLLVLAAAMLPAGLLGDRYGRKKIMLIALAFFGAGSAASAFAPSAGALI
                     AARLVLGLAGAGVIVLALSALTVLFDEAERPRAVGVWAAANFLALPIGPILGGWLLTH
                     YWWGWVFLMNVPVALLGLAATALLVPETRAPRQPGLDLAGIAGSTAGLVLLTYGMIEA
                     GEHGWLDPGALVPMIAGLAVLVGFFLWEWRLSRRPGGQPLLDLALFRSAAFTWGVILA
                     GVAVLAMIGVLFTMPQFFQGVLGTDAMGSGLRLLPLVGGLIAGALPADRVARRLGVKL
                     TAALGFAVLAAGLLLGSATQLPASDRVIAGWMAVVGAGMGLTLATTTSAALVELTAER
                     AGIGSAVLQALSKLGGPFGAAVLGSVLSAAYQSRLRLGGLPSQTAELARSSVFGGVAV
                     ARQLGSPALLDSARAAFTHGMDVALLVSAAIAAAGCLLALLFLPHRGGALPQPEAMRV
                     ESEHELAARR"
     gene            3250..4353
                     /locus_tag="VFU72_05755"
     CDS             3250..4353
                     /locus_tag="VFU72_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5423026.1"
                     /translation="MSNELLARRIERAQEVMRERGVDFLFVAPSSDLVYFFDLAAHAS
                     ERLLLLIIPRQGQAHLVVANFERPHAAGKENLVQLHTWEEHERPLELVHDIVAGVSAP
                     TIGISDQMRAGFLVGMLETLPDAAFVNGTGIIRELRMVKDEDEIAKLKRAAAMADAAW
                     EEFVATAKLTGKTEREAAGELAALRAKHGLEVTSIGICASGPNSAAPHHRTGERVIQP
                     GDSVVFDYGGKYQHYTADVTRTVHIGEPSEEYRHAYDTVLRANEAAVPFYRPGVACQE
                     VDQAARAVIEAAGFGQYFSHRLGHGLGLDGHEEPYLVKGNTLPLRAGMVFSDEPGIYI
                     PGRFGIRIEDSVVVRAGGGEKINHARRDLLVLE"
     gene            4437..>4759
                     /locus_tag="VFU72_05760"
     CDS             4437..>4759
                     /locus_tag="VFU72_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5423027.1"
                     /translation="MPSSADRKRLEVPTHLHERLVEIAIDEDRTVASVVQEIITLGLG
                     QYQSRLAPEAGVGQAPFTERAIRALAFAREEAVPFNHSYLGTEHLLLGLLREGEGVAA
                     RILAR"
BASE COUNT          769 a         1715 c         1674 g          601 t
ORIGIN      
        1 ctgatgctcc tcagctcgta ggcatctggc gcaggagcgg ccgggtcaga caggttgggc
       61 cgccggtgcg gttgtgcgag atctcgtcgc cggcgaagag ggtgacctcg cagccggcgg
      121 cgcgcagccg ctcggcggtg accgtgttct cgcgcagcag caggcagcgg cgcggcgcca
      181 gcgccagcac gttcggcccc tggctggcga actccgcgtc cggcacctcg acgaagcccc
      241 agccgcgcgc ctctagcagc tcgaccagcc gcgagggcat gagcggcaga tagaccaccg
      301 ccaggcgctc gtcgaccggg ctgatcagcg acaggaggtg cagcacctcg ccccgcccgt
      361 gccagtgcgg caggtcgtag gcaagcacct cgacgccctg ctccgccagc agcgcgcgga
      421 gctggtcgat gccggcgtcg ttgctgcggt agccgcgccc gacgatcaag gtgtgctcat
      481 cgagccagaa agtgtcaccc ccttcgagcg tgcccggcgc ctcgatgcgc cccgcgaccg
      541 gcacgcccag ttcggccagc gtctgcgcgc ccagctcgac ctcgcccaag cgcagctcct
      601 tgccgggccg caacaggatc acaccccggt ccgtggtcaa gagcgggtcg aaggggaaga
      661 tggcgtcctg caggcccgcg tcgtcgatct cgcccgcgat cacctcggcg cccgccgcct
      721 gcaggatgcg gcgcaaggcg gcgtgctcgc ggacggccag cgcgtggtcg atcggtcgca
      781 ggtagccgaa ggactcccag gagacctcgg gcgcgggcgg caccggtgga tagaccagca
      841 cccgccgcag cggcgccacc atcgactgcc caccccagga tcggtcgctc atcaacctgc
      901 ctcctgctcc gtgttgctgg cgtccccccc gccgggctct gccgcgagcg gcagccctgc
      961 ctccagatag gccagcgccc ggtccatcag cggccaaaaa tccgcctccg actcggtcgc
     1021 cgccagccag gcggagagcg cgaccccgat caccgcaccg gtgaagacct gcgccgcgaa
     1081 atcgtccggc gagcggccgg tacgccgggc gatggcggcc gtgagcgtct ggatcgtccg
     1141 cgttgactcg ctgagcatgc gcgggtagag ctcgggcgtc gccaggacca ggcgctggcg
     1201 cctggcctcc tgctccagct cctccggcgt catcctcccg gcggtggcgc gcattgcggc
     1261 ccgcagcgcc tgaatcgggt tgagctcggc gggctgcgcg gcgaaggcct ccagcagcaa
     1321 ctcatcgtag tcgtcgcgca gcacgacgtc ttctttagtg gggaaatagc ggaagaaggt
     1381 gctgggcgag acttcggccg cggcggcgat ctgctcgacc gtcgtctcgg cgaagccctg
     1441 ctcgcggaac aggcgtagcg cctgacgctg gatctcggcg cgcgtcctgg ccttcttccg
     1501 ctcacgcagg ctggttgtga ctcgttcatc ggcgggcggc gagctcatgc tccgattcta
     1561 cccgcatcgc ctccggctgc ggtagcgcac cgccgcggtg cggcaggaag aggagcgcca
     1621 gcaggcagcc ggcggcggcg atggcggccg atacgagcag cgccacatcc atcccgtggg
     1681 tgaaggccgc gcgcgccgag tcgagcagcg cgggcgagcc gagctgccgc gccacggcga
     1741 cgccgccgaa gacgctggac cgcgccagct cggcggtttg gctgggcagg ccgccgagcc
     1801 ggagacggct ctggtaggcg gcgctgagca cgctccccag caccgccgcg ccgaatggcc
     1861 cgccgagctt cgacagcgcc tgcagcaccg ccgagccgat gcccgcgcgc tcggcggtga
     1921 gctcgaccag cgcggcggag gtggtggtcg ccagggtgag gcccatcccg gcgccgacca
     1981 cggccatcca gccggcgatg acgcgatcgc tggccggcag ctgggtagcg gagccgagca
     2041 gcagcccggc ggccagcacg gcgaagccga gcgcggccgt cagcttcacg cccaggcggc
     2101 gcgccacccg gtccgccggc agggcgccgg caatcagccc gcccaccagg ggcagcagcc
     2161 gcaggccgga gcccatggcg tccgtaccga gcacgccctg aaagaactgc ggcatggtga
     2221 agaggacacc gatcatggcc agcaccgcca cccccgccag gatcacgccc caggtgaagg
     2281 cggcggagcg aaagagcgcc aggtcgagca acggctggcc gcccggccgg cggctgaggc
     2341 gccactccca gaggaagaaa cccaccagca cggccagccc ggcgatcatc gggacgagag
     2401 cgcctggatc gagccagccg tgctcgcccg cctcgatcat gccgtaggtc agcagcacca
     2461 gtccggcggt cgagccggcg atgccggcga gatcgagtcc cggctgtcgc ggggcgcgcg
     2521 tctccggcac cagcagcgcg gtggcggcca gaccgagcag cgccaccggg acgttcatca
     2581 ggaagaccca gccccaccag taatgcgtca gcagccagcc gccgaggatc ggaccgatcg
     2641 gcagggccag gaagttggcg gccgcccaga ctccgacggc gcgcgggcgc tcggcctcgt
     2701 cgaagaggac ggtcagggcc gaaagcgcca gcacgatcac gcccgcgccg gccaggccaa
     2761 ggacgaggcg cgccgcgatc agggcgccgg ccgagggcgc gaaggcgctg gccgccgagc
     2821 cggcgccgaa gaacgccagc gcgatgagca tgatcttctt gcgcccatag cgatcgccga
     2881 gcaggcccgc cggcagcatc gcggccgcca gcaccagcag gtagccggaa gtgaaccact
     2941 gcaggtccgc ctccgaggcg tgcagcgcgg ccgccagcgt cggcagtgcc acgctgagga
     3001 tggtgccgtc gagccccact gccagcacgg caagcaccag ggcgccgagc gcccaccact
     3061 tgcgcctgtc tgctgttctc atgacattcc ctgccctttc acgcctcagt tctaatgata
     3121 gtctctatca taatcgagtg actgtcaagg agccgacgag cggcgcggtc cgtgagtcgt
     3181 ggcgcccctt gggctggtcg gctatgctgg cgcggcaaac gagcggctga gcgagaagtg
     3241 agggcgagca tgagcaacga gctgctggcg cggcgcatcg agcgggcgca ggaggtgatg
     3301 cgggaacggg gcgtcgattt cctcttcgtc gcgcccagct cggacctggt ctacttcttc
     3361 gatctggcgg cgcacgccag cgagcgcctg ctgctcttga tcatcccgcg ccaggggcag
     3421 gcgcacctgg tggtggccaa cttcgagcgg ccccacgcgg ccggcaagga gaatctggtc
     3481 cagctccaca cctgggagga gcacgagcgc ccgctggagc tggtgcacga catcgtggcc
     3541 ggcgtcagcg caccgacgat cgggatcagc gatcagatgc gcgccggctt cctcgtcggc
     3601 atgctggaga ccctgccgga cgccgcgttc gtcaacggca ccggcatcat ccgcgagctg
     3661 cgcatggtca aggacgagga cgagatcgcc aagctcaagc gcgccgccgc catggccgac
     3721 gccgcctggg aggagttcgt ggccaccgcc aagctgaccg gcaagaccga gcgcgaggcg
     3781 gcgggcgagc tggccgcgct gcgcgccaag cacgggctgg aggtaaccag catcggcatc
     3841 tgcgccagcg gccccaacag cgccgcgccg caccaccgca ccggcgagcg cgtcatccag
     3901 cccggcgact cggtcgtctt cgactacggc ggcaagtacc agcactacac cgccgatgtc
     3961 acccgcaccg tgcacatcgg cgagccgagc gaagagtacc ggcacgccta cgacaccgtg
     4021 ctgcgggcca acgaggccgc cgtgcccttc taccgcccgg gcgtcgcgtg ccaggaagtc
     4081 gatcaggctg cgcgcgccgt gatcgaggcc gccggcttcg gccagtactt cagccaccgg
     4141 ctgggacacg gtctcggcct cgacggccac gaggagccct atctggtcaa ggggaacacc
     4201 ctgccgctgc gcgccggcat ggtcttcagc gacgaaccgg gcatctacat ccccggccgc
     4261 ttcggcatcc ggatcgagga ctcagtggtg gtgcgcgcgg gcggcggcga gaagatcaac
     4321 cacgcgcgcc gcgacctgct ggtgctggag tagcggcgcc aggacttgca acaccgtaaa
     4381 tacggtggta atctgaaaca cgactgatcc gcggcattcg tccgaggagg tgcttcatgc
     4441 catcgagtgc cgaccgcaag cgcctggaag ttccgaccca cctgcacgag cggctggtcg
     4501 agatcgccat cgacgaggac cgcaccgtcg ccagcgtggt gcaggagatc atcacgctgg
     4561 ggctgggcca gtatcagtcg cggctggcgc cggaggcagg cgtgggccaa gcgcccttca
     4621 ccgagcgcgc catccgcgcg ctggccttcg cccgcgagga ggccgtgccc ttcaaccaca
     4681 gctatctcgg caccgagcac ctgctgctcg gcctcctgcg cgagggcgaa ggagtcgcgg
     4741 cgcgtatcct ggcgcggtt
//
LOCUS       DASUBI010000300         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_448625, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000300 DASUBI010000000
VERSION     DASUBI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448625"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..436)
                     /locus_tag="VFU72_05765"
     CDS             complement(80..436)
                     /locus_tag="VFU72_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423028.1"
                     /translation="MGFVMTEEDGFFVPVAVCDHCGREAGPGSLVLWDMAASRAFGAE
                     RRMLVACEDECLEALAGGADDPEGERADERDWVAVALDAYLAGLVERLAIDPAAVLER
                     ERAAAAAEETRREGPE"
     gene            complement(472..873)
                     /locus_tag="VFU72_05770"
     CDS             complement(472..873)
                     /locus_tag="VFU72_05770"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU5423029.1"
                     /translation="MEAPLHLTFFLDENVDLRVGLFLRQQGYTVYSVREHFVPGSPDR
                     AIVAAANAMQAIVVTWNAKHFRPIISRDSVKSYQEFPHAGLIAMTCRPSNALAHRRKW
                     LPSIEFEYRRLQAQADRRLIVEISESGLRIR"
     gene            complement(876..1547)
                     /locus_tag="VFU72_05775"
     CDS             complement(876..1547)
                     /locus_tag="VFU72_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU5423030.1"
                     /translation="MAIVGGFTPVQTAQLTGLSRRQLERWARTGVYKPSLANASRAYG
                     RIYSVRDVVALRALAMMRGKVTLQQIRHAGAWLQRHVDIPWSSLRFSLCERELVLRDA
                     ATGAPLTVWEREPEATAVELGSIAHEVPERLEVELESIAREVVERLPLFQRRQPNHIG
                     RVARNRFVEHNDAVVEGTRIPTQVVWDYHEAGYDTCAILELFPGLTAQDIEAAVAFEQ
                     RHLPS"
     gene            complement(1593..1835)
                     /locus_tag="VFU72_05780"
     CDS             complement(1593..1835)
                     /locus_tag="VFU72_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU5423031.1"
                     /translation="MTYVIAQPCIGLKDGSCVEVCPVDCIHTDDAAEQYYIDPDECID
                     CGVCAEVCPVEAIFFEDDLPEEWTNFLLVNKQYFNK"
     gene            complement(1982..2188)
                     /locus_tag="VFU72_05785"
     CDS             complement(1982..2188)
                     /locus_tag="VFU72_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423032.1"
                     /translation="MPVPMSLPTRVNELSDSALEEQGFSRDEIDRLSLLREMYPYIEY
                     TDTRRQWHRLRFVKWLYNQGQIKK"
     gene            complement(2375..2710)
                     /gene="rpmE"
                     /locus_tag="VFU72_05790"
     CDS             complement(2375..2710)
                     /gene="rpmE"
                     /locus_tag="VFU72_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5423033.1"
                     /translation="MKQGIHPKYQEAQVVCACGNSWTTRSTKPLLRIDLCPQCHPFYT
                     GEQRIVDSAGQVERFMKRMEAAQEQPRRKKVERRQKRLEQRARVQRSTEQLVVEGAEE
                     TEGRAEPAE"
     gene            complement(2735..3010)
                     /gene="rpmA"
                     /locus_tag="VFU72_05795"
     CDS             complement(2735..3010)
                     /gene="rpmA"
                     /locus_tag="VFU72_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5423034.1"
                     /translation="MAHKKGVGSSRNGRESHAKRLGVKRYDGNLVTAGSILVRQRGTR
                     FFAGNNVGMGRDHTLFALIDGSVKWEPISKDRKQVSVYSPVASEPAA"
     gene            complement(3056..3373)
                     /gene="rplU"
                     /locus_tag="VFU72_05800"
     CDS             complement(3056..3373)
                     /gene="rplU"
                     /locus_tag="VFU72_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5423035.1"
                     /translation="MYAVVETGGKQYRVAPGDVIEVEKLASEPGAEITLERVLMVANE
                     GGVTIGTPVVAGARVVASVEDQIKGPKLIVFKMKPKRRYRKKTGHRQKLTRLVIKEIV
                     ASA"
     gene            3647..4630
                     /gene="hemC"
                     /locus_tag="VFU72_05805"
     CDS             3647..4630
                     /gene="hemC"
                     /locus_tag="VFU72_05805"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU5423036.1"
                     /translation="MTTTGDAGLARVRDGEGHTLRAALCVGTRGSALALEQTRRVVAL
                     LQAAAPELTIETRLITTHGDVDKQTPLAVLGGQGVFAKEIEAALLAGEIDLAVHSAKD
                     LTSELPRGLIIAAIPERDDPRDALVAPTPGLRLDDLSPGAHVGTSSRRRALQLQQARP
                     DLVAVELRGNVDTRLRKVRQREGGLAAGILAASGLLRMGWQDAIAEVLPLDTFLPAPG
                     QGALAVECRADDEPLRALLATIDDPAVARAVAIERAFLRAVGGGCRSPIGAFAVIQDE
                     RVLLRAMLGDEENTTALYASRLGALADAEELAAELARGMLQQLAERVAAAR"
BASE COUNT          729 a         1699 c         1553 g          811 t
ORIGIN      
        1 ccgcccgccc gccacgccgc acctcccagc atccgcacgg gcccggatcc acgcccgggt
       61 ccgtgcgcgt tgaagagggc tactccggcc cctcccggcg ggtctcctcg gccgccgccg
      121 cggcccgctc gcgctcgagc acggcagccg ggtcgattgc caggcgttcg accaggccgg
      181 cgaggtaggc gtcgagcgcc accgccaccc agtcacgctc gtccgcgcgc tcgccctctg
      241 gatcgtccgc gcctcccgcc agcgcctcca ggcactcgtc ctcgcaggcg accagcatgc
      301 ggcgctcggc gccgaaggcg cggctggcgg ccatgtccca gaggacgagg ctcccggggc
      361 cggcctcgcg cccgcagtgg tcgcagaccg ccaccggcac gaagaagccg tcctcctcag
      421 tcatcacgaa gcccacgaga aagctgctcc tctcgcccca ccagggagcg actaccgtat
      481 gcgaaggcca ctctcagaga tctcgacaat tagccgtcgg tcggcctgtg cttgcagccg
      541 gcggtactcg aactcaatcg acggcagcca ctttcgtcgg tgtgcgagcg cattgctcgg
      601 ccggcaggtc attgcgatga ggcccgcgtg ggggaactcc tgatagctct tgaccgaatc
      661 gcgggagatg atcggccgga agtgcttggc attccaggtc acgacgatcg cttgcatcgc
      721 gttggcagcg gcaacgatgg cgcgatccgg tgagcccggg acaaaatgct cgcgcaccga
      781 gtagacggta tagccctgct ggcgcaggaa gaggcccact ctgagatcga cgttctcatc
      841 gaggaagaac gtcaggtgga gcggagcctc cactgctaac tcgggagatg ccgctgctca
      901 aaggctacgg cggcttcgat gtcctgggcg gttagaccag gaaagagctc caggatcgcg
      961 caggtgtcgt acccggcttc gtgatagtcc cagacgacct gggtcgggat ccgggttccc
     1021 tcgacgaccg cgtcgttgtg ctccacaaat cgattgcgag cgactcgccc aatgtggttg
     1081 ggctgccgcc gctgaaacag tggaaggcgc tccacgacct cgcgcgcgat tgactccaat
     1141 tcaacttcga gtcgctccgg gacctcgtgt gcgattgacc ccaattcaac tgcagtcgct
     1201 tccggctcgc gttcccacac agtgagaggc gccccggtcg cggcgtcgcg gaggacaagc
     1261 tcacgctcgc agagggagaa tcgcaggctc gaccagggga tatcgacatg ccgctggagc
     1321 caggcgccgg catgccgaat ctgctggagc gtcaccttgc ctcgcatcat ggcgagcgcg
     1381 cgtagcgcga ctacatcgcg gacggaatag attcggccgt aggcacgcga agcgttcgcc
     1441 aggctcggct tatacacacc cgtgcgcgcc cagcgctcca gctgtcgtct gctcagtccg
     1501 gtcagttgag cggtctgcac cggggtgaac ccgcccacga tagccatggc ttccagcctt
     1561 ccgggcccac ctggctccag ctacgtcgcc agttacttat tgaaatactg cttattcacc
     1621 aggaggaagt tcgtccactc ctcggggagg tcgtcctcga agaagatcgc ctcgaccggg
     1681 cagacctcgg cgcagacgcc gcagtcgatg cactcgtcgg ggtcgatgta gtactgctcg
     1741 gccgcgtcgt cggtgtggat gcaatcgacc gggcagacct cgacgcagga gccgtccttg
     1801 aggccgatgc agggctgggc gattacgtag gtcaccgaaa gatcctttcc gtcgctcatc
     1861 gcgaggtcat ggtcatggtc atgggtcgcg cgccgtcagc gttcgagtat acccgaccgg
     1921 cacacgcgcg gggcagccgc ccggctgccc cgcacccact gcctgatcga cgagcgcccg
     1981 tctacttctt gatctgcccc tgattgtaga gccacttcac gaagcgcagg cggtgccact
     2041 ggcgccgcgt gtcggtgtac tcgatatagg ggtacatctc acggaggaga ctgagccgat
     2101 cgatctcatc cctgctgaac ccctgctcct ccaacgccga atccgacagc tcgttgacac
     2161 gagtcgggag agacattggt acaggcatcg ctcgctcctc ctcttggggc ggcgtctccg
     2221 ccgcaccgga cagaaactgc cctctgttaa tattgtacga cgccgggcga ttcctggttc
     2281 aggcggccaa ccgcgcgtac cacctgcacc ataaacgaat cggcgcggct gagagccgcg
     2341 ccgaccgtcc cgatttcgga ttggttcctg gtacttactc ggccggttcg gcccggccct
     2401 cggtctcttc ggctccctcg accacgagct gctccgtgct cctctgcacc ctggcacgct
     2461 gctcgagccg cttctgccgc cgctccacct tcttgcggcg cggctgctcc tgggccgcct
     2521 ccatgcgctt catgaagcgc tcgacctgcc ccgcgctgtc cacgatgcgc tgctcgcccg
     2581 tgtagaacgg gtggcactgc gggcagaggt cgatgcgcaa cagcggcttg gtggaccggg
     2641 tggtccagct attgccgcag gcgcagacga cctgggcttc ctgatacttc ggatggattc
     2701 cctgcttcac tcgactaccg tccttcggct gcccttaggc tgccggctcg ctcgccaccg
     2761 gcgaatagac actgacctgc ttgcggtctt tgctgatcgg ctcccacttg accgagccgt
     2821 cgatcagcgc gaagagcgta tgatcgcggc ccatgccgac gttgtttccg gcgaagaagc
     2881 gggtgccccg ctgccgcacc aggatcgagc cggcggtcac caggttgccg tcgtagcgct
     2941 tgactcccag acgcttggcg tggctctcgc ggccgttgcg cgagctgccg acgcccttct
     3001 tatgcgccat atctctcctc catgccccgg ataccctgga tccgcgccgg ccgcgctagg
     3061 cgctggccac gatctccttg atcaccaggc gcgtcagctt ctggcggtgg ccggtcttct
     3121 tgcgatagcg ccgcttgggc ttcatcttga agacgatcag cttcgggccc ttgatctggt
     3181 cctcgacgct ggccactacc cgggcgcccg cgacgaccgg cgtgccgatc gtgacaccgc
     3241 cctcgttcgc caccatcagc acccgttcga gcgtgatctc cgcgcccggc tcagaagcca
     3301 gcttctcgac ctcgatcacg tcgcccggcg cgacccggta ctgctttccg cctgtttcga
     3361 cgactgcgta catggttcgt gcttcaatcc tcactgctgc cgtagacggc caaagccggc
     3421 gtgcgacacc cgcgtaatgt tcgcagacaa aagcggtggg cgcacgcgcc cacgacctat
     3481 gaaggctatc aaacacctgc cgctttgtca aacatctccc gaccaccccg ccttgacacg
     3541 tcattcggct gctccgtaga atggcatcgg tgcatagttt tgcacagatt tatagagttt
     3601 tccatagatt ttgcgccggg cagccagcct gggagggagc cgaagggtga cgacgaccgg
     3661 cgacgccggg ctggcgagag tgcgcgacgg ggaggggcac acgctccgcg ctgcgctctg
     3721 cgtcggcacg cgcggcagcg cgctggcgct ggagcagacc cggcgtgtgg tggcgctcct
     3781 gcaggccgcc gcgcccgagc tgaccatcga gacccgcctg atcaccaccc acggcgatgt
     3841 cgacaagcag acgccgctcg ccgtcctcgg cgggcaaggg gtcttcgcca aggagatcga
     3901 ggccgcgctt ttggccggcg agatcgatct ggcggtccac agcgccaagg acctgacctc
     3961 ggagctgccg aggggcctga tcatcgccgc catccccgag cgcgacgatc cgcgcgacgc
     4021 gctggtcgcg ccgaccccgg gcctccgcct cgacgatctg tcccccggcg cccacgtcgg
     4081 caccagctcg cgccgccggg cgctgcaatt gcagcaggcg cggcccgatc tggtcgcggt
     4141 cgagctgcgc ggcaacgtcg atacccggct gcgcaaggtg cgccagcggg aaggcgggct
     4201 ggccgccggc atcctggccg cgtccgggct gctgcgcatg ggctggcagg acgcgatcgc
     4261 tgaggtcttg ccgctcgaca ccttcctgcc ggcgccgggc cagggcgcgc tggcggtcga
     4321 gtgccgcgcc gacgacgagc cgctgcgcgc gctgctggcg accatcgacg atccggccgt
     4381 cgcgcgcgcg gtggcgatcg agcgcgcctt cctgcgcgcg gtgggcggcg gctgccgctc
     4441 gccgatcggc gccttcgccg tgatccagga cgagcgcgtg ctgctgcgcg ccatgctcgg
     4501 cgacgaggag aacaccaccg cgctctacgc cagccgcctg ggcgccctgg ccgacgccga
     4561 ggagctggcg gccgagttgg cgcgggggat gctgcagcag ttggccgagc gggtggcggc
     4621 cgcgcgatga gcgcgccgct ggccggcaag cgcgtcctcg tcacccgggc gcggggacag
     4681 gccggcgcgc tggcgcagga gctgcgcgcg gccggcgccg agccggtcga gctgccggtg
     4741 atcgccttcg ccccgccgcg cgaccccgcc ccgctggcgg ccgccctggt ac
//
LOCUS       DASUBI010000301         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_560786, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000301 DASUBI010000000
VERSION     DASUBI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560786"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFU72_05810"
     CDS             complement(<1..644)
                     /locus_tag="VFU72_05810"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEU5423037.1"
                     /translation="MEVQLRIQRYNPEVDSKPHYEEYTVDAEPDDRVLDALNQIKWHQ
                     DGTLTFRRSCAHGVCGSDAMRINGRNRLACKILIKDIGSKITIEPLLGFDVIKDLVID
                     FEPFFASYRKVMPYLVNDDSPPATERIQTPAERELYEDTSKCILCGACTTACPIIWTT
                     EWVGPAAIVNAHRFIFDSRDQAGEDRLRILNDGQGVWRCRTIFNCTDACPRGIQV"
     gene            complement(644..2371)
                     /gene="sdhA"
                     /locus_tag="VFU72_05815"
     CDS             complement(644..2371)
                     /gene="sdhA"
                     /locus_tag="VFU72_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEU5423038.1"
                     /translation="MYHRFDAIVVGAGGAGLMAVAQLAGKANVAVISKLYPVRSHTGA
                     AQGGIAAALGNLEEDHWVWHMFDTVKGGDYMVDQDAAEVLAREAVDAIYDLEHMGFPF
                     NRTPDGRIDQRRFGGHTRNFGEGPVRRSCYAADRTGQMILYTLYQQGIKHNVRFFNEL
                     HMLDLLLTDDGACAGLVGMDVQTGEIHVFHTKAVLLATGGYGRVFKTTSNALTLAGDG
                     MAIASRRGLPLEDMEFYQFHPTGIAYKGILLSEACRGEGGILRNDAGERFMERYAPTL
                     LDLAPRDMVSRSIEMEIRAGRGIGGRDYVHLDLTHLPPEVIETKLPDITDFVRTYQGL
                     DPIKDLIPIQPTAHYAMGGIPTDVEGRVTIDENNTILPGLYAAGEVACVSVHGANRLG
                     TNSLVDLVVFGRRAGRHMLDYVQQSDFHPLPKEPEFRARAEVAAFLDGPGDENAADIR
                     TEMQSVMWDDCGIFRSGEGLTELRTKLTELRNRFSRVGTGDKGKTFNLELFEVLELGY
                     LLDLAEIMAESALRRHESRGAHVREDFPDRDDKNWLKHTLAYRTSGGLEMRYKPVVLT
                     RFEPKERVY"
     gene            complement(2382..2789)
                     /locus_tag="VFU72_05820"
     CDS             complement(2382..2789)
                     /locus_tag="VFU72_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU5423039.1"
                     /translation="MVALPRVGRERPTGGNFELYSWYFLRVSGILLIFLAVVHVIIMH
                     VIHQVSDIDFQFVANRWNSPFWRFFDWLLLTLALLHGLNGARIAIDDYIHARGWRIIA
                     LSALWSAAIIFLILGSVAIVTFNPANFNSVAGR"
     gene            complement(2815..3168)
                     /gene="sdhC"
                     /locus_tag="VFU72_05825"
     CDS             complement(2815..3168)
                     /gene="sdhC"
                     /locus_tag="VFU72_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU5423040.1"
                     /translation="MVSSYRGYRAPQGMLSWALQRLSGLGILAFLLLHIIDIFIIGYG
                     PKDFNDLLFLYHNPIFKIGELILVAAVYYHAANGVRIILIDFWRSAYRYERQLWWIVW
                     IAFVVAMIPTTIRMF"
     gene            3496..>3820
                     /locus_tag="VFU72_05830"
     CDS             3496..>3820
                     /locus_tag="VFU72_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5423041.1"
                     /translation="MSGVYVALDLEATGMDPERDEIIEVAAIKFRDDRVLDRFESLVR
                     PQSSQIPFNISSLTGIGAKDVRRAPLFSVVAPRLRDFVRNFPIVGQSPQFDVEMLVGA
                     GLALQN"
BASE COUNT          673 a         1163 c         1315 g          669 t
ORIGIN      
        1 acctggatgc cgcgcgggca ggcgtcggtg cagttgaaga tggtgcggca gcgccagacg
       61 ccctggccgt cattcaagat acggaggcga tcctcaccgg cctgatcgcg gctgtcgaag
      121 atgaagcggt gggcgttgac gatggcggcc gggccgaccc actcggtagt ccagatgatc
      181 gggcaggcgg tggtgcaggc gccgcagagg atgcacttgc tcgtgtcctc gtagagctcg
      241 cgctcggccg gggtctgaat gcgctcggtc gccggcggag aatcgtcgtt gaccagatac
      301 ggcatcacct tgcggtagga cgcgaagaac ggctcaaagt cgatgacgag gtccttgatc
      361 acgtcgaagc cgagcaacgg ctcgatggtg atcttactgc cgatgtcctt gatcaggatc
      421 ttgcaggcga ggcggttgcg gccgttgatg cgcatggcgt cggagccgca gacgccgtgg
      481 gcgcaggagc ggcggaaggt cagcgtgccg tcctggtgcc acttgatctg gttgagcgcg
      541 tcgagtacgc ggtcgtcggg ctcggcgtcg acggtgtact cctcgtagtg cggcttgctg
      601 tcgacctcgg ggttgtagcg ctggatacgg agctgcactt ccatcagtag actcgttcct
      661 ttggctcgaa gcgggtcagc acgacaggct tgtagcgcat ctccaggccg cccgaggtac
      721 ggtaggccag cgtgtgcttc agccagttct tgtcgtctcg atccgggaag tcctcgcgaa
      781 cgtgggcgcc gcggctctca tggcgacgca gcgcgctctc ggccatgatc tccgcgagat
      841 cgagcaggta gcccagctcg agcacctcga agagttccag attgaaggtc ttgcccttgt
      901 cgccggtgcc gacgcggctg aagcggttgc gcagctcggt cagcttggtc ctcaattcgg
      961 tcaggccctc gccgctgcgg aagatgccgc agtcgtccca catcacgctc tgcatctcgg
     1021 tgcggatatc ggccgcgttc tcatcacccg gtccatcgag gaacgcggcg acctcggcac
     1081 gggcgcgaaa ctccggttcc ttgggcagcg ggtgaaaatc gctctgctgc acgtagtcga
     1141 gcatgtggcg cccggcgcgg cggccgaaga cgaccagatc gacgagcgag ttggtgccga
     1201 gccggttggc gccgtggacg ctgacgcagg ccacctcacc cgcggcgtag aggcccggta
     1261 ggatggtgtt gttctcgtcg atggtgacgc ggccctccac atcggtgggg ataccgccca
     1321 tcgcgtagtg cgccgtcggc tggatcggga tcagatcctt gatcgggtcg aggccctggt
     1381 aggtgcgcac gaaatcggtg atgtcgggca gtttggtctc gatcacttcg ggcggcaggt
     1441 gggtgaggtc gaggtggacg tagtcccggc cgccgattcc gcgcccggcg cgaatctcca
     1501 tctcaatgct gcgcgagacc atgtcgcgcg gggccagatc gagcaaggtg ggggcatagc
     1561 gctccatgaa gcgctcgccg gcgtcgttgc gcaggatacc gccctcgccc cggcaggcct
     1621 ccgagagcag gatgcccttg taggcgatac cggtggggtg gaactggtag aactccatgt
     1681 cctccagggg cagcccgcgc cgcgaggcga tcgccatgcc gtcgccggcc agcgtgagcg
     1741 cgttgctggt ggtcttgaag acgcgcccgt agccgccggt ggccagcagc acggccttgg
     1801 tgtgaaagac gtggatctcg ccggtctgca catccatgcc gaccagaccg gcgcaggcgc
     1861 catcgtcggt gagcaacagg tcgagcatgt gcagctcgtt gaagaagcgc acgttgtgct
     1921 tgatcccctg ctggtagagc gtgtagagga tcatctggcc ggtgcggtcg gcggcatagc
     1981 aggagcgccg caccgggccc tcgccgaagt tgcgcgtgtg gccaccgaag cgacgctgat
     2041 cgatgcgacc gtcgggggtc cggttgaagg ggaaccccat gtgctccagg tcgtagatgg
     2101 cgtcgaccgc ctcgcgcgcc agcacctcgg cggcgtcctg gtcaaccatg tagtcgccgc
     2161 ccttgacggt gtcgaacatg tgccagaccc agtgatcttc ttcgagattg ccgagggcgg
     2221 cggcgatgcc gccctgcgcc gcgccggtgt gcgagcgtac cgggtagagc ttgctgatga
     2281 cggcgacgtt ggccttgccg gcgagctgcg ccaccgccat caggcccgca ccgccggcgc
     2341 cgacgacgat ggcgtcgaat cggtgataca tcgtctctct cctatcgacc ggcgacgctg
     2401 ttgaagttcg ccgggttgaa cgtgacgatc gccaccgagc caaggatcag gaagatgatg
     2461 gccgcgctcc agagcgccga gagggcgatg atgcgccagc cccgcgcgtg aatgtaatca
     2521 tcaatcgcga ttcgcgcgcc attgagcccg tgcagcagcg ccagcgtcag cagcagccaa
     2581 tcgaagaagc gccagaacgg agagttccag cggttggcga cgaactggaa atcgatgtcg
     2641 ctgacctggt gaatgacgtg catgatgatg acgtggacga cggccaggaa gatcaggagg
     2701 atgccgctga cccgcaagaa gtaccaggaa tagagctcga aattgccgcc ggtggggcgc
     2761 tcgcgtccga cccgaggaag agctaccacg atcgtaccct ctctcgcttc cgcgctagaa
     2821 catccggatc gtggtcggga tcatggcgac cacgaaggcg atccagacga tccaccagag
     2881 ctggcgctcg tagcggtagg cgctgcgcca gaaatcgatc aggataatgc gcacgccgtt
     2941 ggcggcatgg tagtagaccg ccgcgaccag gatgagctcg cctatcttga agatcgggtt
     3001 gtgatagagg aacaggagat cgttgaagtc cttggggccg taaccgatga tgaagatgtc
     3061 gatgatatgc aggagcaaga aggccaagat gccgaggccc gagagacgct gcagcgccca
     3121 ggagagcatg ccctgcggcg cgcggtaccc tctgtagctt gagaccactc cgcccgatcc
     3181 cttcgctagg ccgatccaga cgcgacctcg ttgtcgtgcc agcggtcatt gtattccgca
     3241 ccatttttgt gtcaacagcg ccgcccccgt ccttgctata ctgcccgagg aagtgggagc
     3301 ggcagcgcgg ggatagctca accgccggtg atcggtgagt ttctcggtag cggccggtgg
     3361 tgccgcctcg gataccaagc gcgaccggtg cagatgaccg tcggcccgcc cacccactag
     3421 ccgggaggca tgacctcggc tagactggtg tctagcccac agcggagcca ccccggaact
     3481 agaaaggccg gcccggtgag cggagtctac gtcgcgctcg atctggaagc gacggggatg
     3541 gaccccgagc gcgacgagat catcgaggtg gccgcgatca aatttcgcga cgatcgcgtg
     3601 ctcgaccgct tcgagagcct ggtacgcccg caaagcagcc agatcccgtt caatatcagc
     3661 agcttgaccg gcatcggggc caaggacgtg cgccgcgcgc cgctcttcag tgtggtcgcg
     3721 ccccggctgc gcgacttcgt gcgcaacttc ccgatcgtcg gccagtcgcc gcagttcgat
     3781 gtcgaaatgc tggtaggcgc cggactggcg ctgcagaacc
//
LOCUS       DASUBI010000302         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_824571, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000302 DASUBI010000000
VERSION     DASUBI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824571"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3342
                     /locus_tag="VFU72_05835"
     CDS             <1..3342
                     /locus_tag="VFU72_05835"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEU5423042.1"
                     /translation="WYSGDVTIQWTCSDATSGLDGTCPVDSTIIGEGGNLSASATVSD
                     LAGNSTTMTVDHIQIDRTAPATSASITGNLHNGWYNATIQIALTATDNLSGVDATYYS
                     IDGGAPQAYSGQVSFGSEGTHTLTYWSVDKAGNVEDKTGNSITLKIDTTAPTLSGTPT
                     TQPNGNGWYSGDVTITWTCADTGSGLDGTCPADSTISGEGDNLGASASISDLAGNSIT
                     TTVDHIQIDRTAPSTTASVPDAPASGWYTDDVPVTLTGHDSLSGVAATYYSVDGGAAQ
                     LYSGPFSFGLEGTHSISFWSEDKAGNAETPGSPLTLEIDKTAPTTTVINPISPASGWF
                     VTSGISFAFQATDNASGIAATYYTIDGGAPQTYGQPFTANLSDGSHLITYWSVDLAGN
                     VETHATTNTVQVNVDTTPPTITGSQSPVANGFGWNNTDVSVTFTCGDAAPGSGLQTGV
                     AGCSGDTTLTNEGADQMVRGNAVDVAGNTSSTDFGPVNIDKTVPTLSGAATTNPNAFS
                     WYKGDVKIHWTGQDGLSGIDPATQPVDSVITGEGANLGAGPVSISDKAGNAGSGSVSG
                     IKIDRTAPAIGGATVNDDGTPRSPNGAGWFNSAVRVRFSCSDALSGVQECASDVVLST
                     DGANQSASGSASDKADNSASATVSGINIDSQAPQTSANNQCDSKNGWCKGQTATVVLT
                     ATDQLGLSGVKEIHYFVNGGPEKIASGASVNVNVPLAAKSGIATVEYFAVDNAGNGEA
                     KTGVSLKYDNIAPSVTHTLNPPANAAGWNNADVTVHFAATDDDGGSGVDPATVTQDQT
                     ISSETTGQVVNGSASDLAGNLGTDSVTVKLDKTAPTISGTATTSPNAGGWYNGPVTVH
                     FTCAEQGAVQSGVATCPADVVLSGDGANQSASGTASDKAGNTASATVSGINIDSTKPT
                     INSISVVNGAIYTLGDPATPSGQLSCSAGDGGSGVASCTVSVTGGNANGVGTFSYTAT
                     ATDNAGNTATLTGSYKIIYRWDGFLQPINDTAHQVGTTTSIFKGGSTVPVKFQLKKAD
                     GTIIQSNTTPQWLTPAKGSATTAPVDETVYSDPATSGSTYRYDSTAQQYIYNWGTAKN
                     QAGYYWRIGVQLDDGQIYYVNIGLR"
     gene            complement(3537..4286)
                     /locus_tag="VFU72_05840"
     CDS             complement(3537..4286)
                     /locus_tag="VFU72_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5423043.1"
                     /translation="MNAAREITRYEILGVRLDDVTLEEAVALIEGFIAAGGPHLVATT
                     NTEFVMAAQTDPAFRRVLNEAALSLPDGIGLIWGLRLLGENVREHVRGTDLVERLLAV
                     AAEHGQRVFLLGAAEGVAEAAAARLRQRYPTLQIAGCYAGSPEPAHDAEARAAIRAAG
                     PVDILLVAYGGGRQEQWIARNLEPLGIPVGIGVGGVFDFLAGRVPRAPRWVRRLELEW
                     LYRLIRQPWRWRRQLALPHFALMVLAAKLRR"
     gene            complement(4283..>4583)
                     /locus_tag="VFU72_05845"
     CDS             complement(4283..>4583)
                     /locus_tag="VFU72_05845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEU5423044.1"
                     /translation="AHVARIERETDALSAEVELPPHFILPGDSVPGAALDVARAVVRR
                     AERLAVKLAHDGVVHNPEVLAYLNRLSSLLFILARFEDREAGVTPTPAKEERPRR"
BASE COUNT          856 a         1627 c         1508 g          592 t
ORIGIN      
        1 tggtacagcg gcgacgtcac catccagtgg acgtgctcgg atgccacatc cggcctcgat
       61 ggcacctgcc cggtcgatag cacgatcatc ggcgagggtg gcaacctgtc cgcgtccgcc
      121 accgtcagcg acctggccgg caacagtact acgatgactg tcgatcacat ccagatcgac
      181 cgcaccgctc cagctacgtc ggcgagtatc accggcaacc tccacaacgg atggtacaac
      241 gccaccatcc agatcgcgtt gacggctacc gacaacctct cgggcgtcga cgcgacctac
      301 tattcgatcg acggcggggc gccgcaggcc tactccggtc aggtctcctt cggaagcgaa
      361 ggcacgcaca ccctcaccta ctggagcgtg gacaaggcag gcaacgtcga ggacaagacg
      421 ggcaacagca tcacgctcaa gatcgacacg accgcgccga cgctcagtgg cacgccgact
      481 actcagccga acggcaacgg ctggtacagc ggtgacgtca ccatcacctg gacctgcgcg
      541 gataccggtt ccggcctcga tggcacctgc ccggccgata gcacgatcag cggcgagggc
      601 gacaacctcg gtgccagcgc ctcgatcagc gatctggccg gcaacagcat cacgacgacg
      661 gtcgatcaca tccagatcga ccgcacggcg ccatcgacta cggcaagcgt ccccgatgcg
      721 ccggccagcg gctggtacac cgacgatgtc ccggtcacgc tgaccggcca cgactcgctc
      781 tcaggcgtcg ccgcgaccta ctacagcgtc gacggcgggg cggcgcagct ctacagcggt
      841 cccttctcct tcggcctgga gggaacgcac agcatcagct tctggagcga ggataaggcc
      901 ggaaacgccg agacccccgg cagcccgctg acgctggaga tcgacaagac cgcgccgacg
      961 acgacggtga tcaacccgat ctccccggcc agcggctggt tcgtcaccag cggcatctcc
     1021 ttcgcgttcc aggcgacgga caacgcgtcg ggtatcgccg cgacctacta caccatcgac
     1081 ggcggcgccc cgcagaccta cggccagccg ttcaccgcga acctgtccga cggcagccac
     1141 ctgatcacct actggagcgt ggacctcgcc ggcaacgtgg aaacccatgc gaccacgaat
     1201 acggttcagg tcaacgtcga caccacgccg ccgacgatca cgggcagcca gtcgccagtc
     1261 gcgaatggct tcggctggaa caacaccgat gtcagcgtaa ccttcacctg tggcgacgcg
     1321 gcccccggct ccggtctcca aaccggtgtc gccggttgca gtggcgacac gaccctcacc
     1381 aacgagggag cggatcagat ggttcgcggt aacgcggtcg acgtggcggg caacacgagc
     1441 agcaccgatt tcggcccggt caacatcgac aagaccgttc cgacgctgag cggcgcggcg
     1501 acgacgaacc cgaacgcctt tagctggtat aagggcgacg tcaagatcca ttggaccggc
     1561 caggacggcc tgtccggcat cgacccggct acgcagccgg tcgatagtgt gatcaccggc
     1621 gagggcgcca acctcggcgc cggcccggtc agtatcagcg acaaggccgg caatgccggc
     1681 tccggctcgg tcagcggcat caagatcgac cgcaccgcgc ccgcgatcgg cggcgcgacc
     1741 gtcaacgacg acggcactcc ccgtagcccc aacggcgccg gctggttcaa cagcgccgtg
     1801 cgcgtgcgct tcagctgcag cgacgcgctc tccggcgtgc aggagtgcgc cagtgacgtg
     1861 gtcctgagca cggacggcgc caaccagagc gcgagcggct cggccagcga caaggccgac
     1921 aacagcgcca gcgcgacggt cagcggcatc aacatcgaca gccaggcgcc gcaaaccagc
     1981 gccaacaacc agtgcgacag caagaacggc tggtgcaagg ggcagacggc gaccgtcgtg
     2041 ctgacggcga ccgatcagct cggcctctcc ggtgtcaagg agattcacta cttcgtcaac
     2101 ggtgggccgg agaagatcgc cagcggcgcg agcgtaaacg tcaacgtgcc tcttgccgcc
     2161 aagtcgggta tcgcgaccgt cgagtacttc gcggtcgaca acgctggcaa cggggaggcg
     2221 aagaccggcg tctcgctcaa gtacgacaac atcgctccgt ccgtgacgca tacgctgaac
     2281 ccgccggcga acgcggccgg ctggaacaac gccgacgtca ccgtgcactt cgctgcgacg
     2341 gacgacgatg gcggttcggg cgtcgatccg gcgacggtca cccaagatca gacgatcagc
     2401 agcgagacga ccggtcaggt cgtgaacggt tccgccagcg acctggccgg caacctcggc
     2461 accgactcgg tgaccgtcaa gctcgacaag accgcgccga ccatcagtgg gacggccacg
     2521 acgagcccca acgccggcgg ctggtacaac gggccggtga cggtccactt cacctgcgcc
     2581 gagcagggcg ccgtgcaatc cggggtcgcg acctgcccgg ctgacgtggt gctcagcggc
     2641 gacggcgcca accagagcgc gagcggcacg gccagcgaca aggccggcaa taccgccagc
     2701 gcgacggtca gcggcatcaa catcgactcg accaagccga cgatcaattc gatcagcgtc
     2761 gtcaatggcg cgatctacac gctcggtgat ccggcgacgc cgtccgggca actgagctgc
     2821 agcgccggcg atggcggctc gggtgtcgcc tcctgcacgg tcagcgtgac gggcggcaac
     2881 gctaacggcg tcggcacctt cagctacacg gccacggcga ccgacaatgc cggcaacacg
     2941 gccacgctga ccggcagcta caagatcatc taccgctggg atggcttcct gcagccgatc
     3001 aacgacaccg cgcatcaggt cggcacgacg accagcatct tcaagggtgg cagcaccgtg
     3061 ccggtgaagt tccagctcaa gaaggcggat ggaaccatca tccagtcgaa cacgactccg
     3121 cagtggctca cgcccgcgaa gggcagtgcc accaccgcgc cggttgacga gaccgtctac
     3181 agcgatccgg cgacgagcgg cagcacgtac cgatacgaca gcacggccca gcagtacatc
     3241 tacaactggg ggacggcgaa gaaccaggcc ggctactact ggcggatcgg ggtccagctg
     3301 gacgacggac agatctacta cgtcaacatc gggctgcgct gagccgaagc tgtaggccga
     3361 tatgtgcgag cggacccgtg caggcgggtc cgctccttct ttctccatac cgggtcgggg
     3421 agatcgcgcg ggccgtcgcc cggcagattg agcgagacga cggtgagcat gatcgctagg
     3481 ccgggcacca tggtcggcac cgggcgcgaa ctcgctgtcc gcccgtaccg cctggctcag
     3541 cgacgcagct ttgccgccag caccatcagc gcgaagtggg gcagggcgag ctggcggcgc
     3601 cagcgccagg gctggcggat caggcggtag agccactcca gctccagccg gcgcacccag
     3661 cgcggggcgc gcggcacgcg ccccgccagg aagtcgaaga ccccgccgac gccgatcccg
     3721 accgggatcc ccagcggctc caggttgcgc gcgatccact gctcctgccg gccgccgccg
     3781 taggccacca gcaggatatc gacgggcccg gcggcgcgga tcgccgcccg cgcctcggcg
     3841 tcgtgcgccg gttccggcga gccggcgtag caaccggcga tctggagcgt ggggtagcgc
     3901 tggcgcagcc gcgccgccgc cgcctcggcc accccctcgg ccgcgcccag caggaagacg
     3961 cgctgtccgt gctcggccgc caccgccagc agccgctcga ccaggtcggt gccgcgcacg
     4021 tgctcgcgca cgttctcacc taagagccgc aggccccaga tcagcccgat gccgtcgggc
     4081 agcgagagcg cggcctcgtt cagcacgcgg cggaacgcgg ggtccgtttg cgcggccatg
     4141 acgaactcgg tgttggttgt ggcgaccagg tgcgggccgc ccgcggcgat gaagccctcg
     4201 atcagcgcga cggcctcctc cagcgtgaca tcgtccaggc gcacgccgag gatctcgtag
     4261 cgggtgatct ccctggccgc gttcaccggc gcgggcgctc ctccttggcg ggcgtcggag
     4321 tgacgccggc ctcgcggtct tcgaagcgcg ccaggatgaa gaggagcgag gagaggcggt
     4381 tgaggtaggc cagcacctcg gggttgtgca cgaccccgtc gtgggccagc ttgaccgcca
     4441 gccgctcggc gcggcgcact accgcgcggg ccacgtcgag cgcggcgccg ggcacgctgt
     4501 cccccggcag gatgaagtgg ggcggcagct cgacctcggc gctgagcgcg tcggtctcgc
     4561 gctcgatccg ggcgacgtgt gcg
//
LOCUS       DASUBI010000303         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_2436, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000303 DASUBI010000000
VERSION     DASUBI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFU72_05850"
     CDS             complement(<1..465)
                     /locus_tag="VFU72_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5423045.1"
                     /translation="MFDAFGLGIFCADLEGRFTYVNPAAQALLGWTGEELLGRVVHDT
                     IHHSRPDGSPFAREDCVLHQALERVAAQQEQDDQFWRKDGAPLEIAQTIAPLREKDKL
                     IGAIVVFVDVSERRRHADALRRRATQQSALTELGLLALGDESLQALIDQCTAR"
     gene            complement(533..1177)
                     /locus_tag="VFU72_05855"
     CDS             complement(533..1177)
                     /locus_tag="VFU72_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423046.1"
                     /translation="MPSKARIRVYLVDDHPMFLRGIVQEVKLRPELELVGQATNGRQA
                     LADLRSLQPDVALIDMRIPGLSGLEVLAAARRDALKTRVVFLSAHLDGDLVYRAIASG
                     ASGYLSKEADRDQILDAVTAVARGQVVLAPEGQTGLAGEVRRREVVERPILTPREREV
                     LVMTADGLTAPEIAQRLQLGVATVRSHLQNVYEKLEVSDRAAAVAEAMRAGLLE"
     gene            complement(1428..2222)
                     /locus_tag="VFU72_05860"
     CDS             complement(1428..2222)
                     /locus_tag="VFU72_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5423047.1"
                     /translation="MESVANTLALAIDRRKTEEELWQRNAEMVKLTRQLTRLADERRR
                     IMADALDAEDRARERISQALHDEVLQSLLSARQDLAKARRDKSGGDHALRDAAEAVVE
                     AIGNLRSAVVALHPVTRARGGIAAAIRAVADLHAKRGGLEVTLSLQPQTSGVYDQLIV
                     SLVGELLSNVAQHAEATQVTVSLRRTREQILLEVADNGRGIEPGRAEEALGQGHIGLA
                     SIAMRVESCGGRFELSATPGEGTRARAVLPAARTGGSSRSGGASRD"
BASE COUNT          366 a          800 c          740 g          389 t
ORIGIN      
        1 ccgcgcagtg cactgatcga tcaacgcttg cagcgattcg tcgccgagtg ccagcaggcc
       61 cagttcggtc agagccgact gctgcgtcgc tcgccgacgg agtgcgtcag catggcggcg
      121 ccgctcactc acgtcgacga acacgacgat cgcaccgatc agcttgtcct tttcccgcag
      181 cggcgcaatc gtctgcgcga tctccagggg tgcgccgtcc ttgcgccaga actgatcgtc
      241 ttgctcctgc tgtgccgcga cgcgctcgag cgcttgatgc agcacacagt cctcgcgcgc
      301 aaacggcgag ccgtcaggac gggaatggtg aatcgtgtcg tgcacgacgc gcccgagcaa
      361 ctcctcgccg gtccagccca ggagcgcttg ggcggctggg ttgacgtagg tgaacctgcc
      421 ttcgagatcc gcgcagaaga ttccgagtcc gaaggcgtcg aacaccgaga cgacgtcgga
      481 gacgccgcgc ttgaggatcg acgggatttt cagcaggcgc tcaggcagcg gttcactcca
      541 gcagccctgc gcgcatcgcc tccgcgacgg ccgccgcccg gtcagacact tcgagctttt
      601 cgtagacgtt ctggaggtga ctcctgaccg tggccacccc gagctgcagc cgctgggcta
      661 tctccggcgc cgtgaggccg tccgcggtca ttaccaagac ctctctctcc cggggtgtca
      721 ggatcggtcg ctcgaccact tcgcgtctgc gaacctcccc ggcgagaccg gtctgacctt
      781 cgggggcgag caccacctga ccgcgcgcga cagcggtcac ggcatcgagg atctgatcgc
      841 ggtcggcctc cttcgagaga tagccggatg cgccgcttgc gattgcgcgg tagaccaagt
      901 cgccgtcgag gtgcgctgag aggaacacaa cgcgcgtctt gagggcgtcg cgcctggccg
      961 ccgcgagcac ctcgagcccc gacaacccgg gaatacgcat gtcgatcaga gccacgtctg
     1021 gctgcagcga ccgaaggtcg gcaagcgcct ggcgcccgtt cgtagcctgg ccgaccagct
     1081 ccagctccgg acgcagcttc acctcctgga cgatcccgcg caggaacatc ggatgatcgt
     1141 caaccagata gacccggatg cgcgccttcg acggcacgcc gtcacaccgc tgtcgcgggc
     1201 cgcccgacgc gccggggcgg cagtgtttct gggacgccaa caggccgccc gatctgcgag
     1261 gtgccgttgt gaaacttcaa gaccggtatt cctctgatga agcctaattc gttgttctcg
     1321 tgcaccgcat gggaacaggc gcaggccggg gctcctcccg aatatggcag cggttgcacc
     1381 gccgcgccat cccacgaccg caggaccgag cagcggtggc tcctcgctca atcgcgggat
     1441 gcgcctcctg atcggctcga gccgccagtc cgtgcggctg ggagcacagc ccgtgcacgc
     1501 gtaccctcgc caggggtggc gctgagctcg aagcgtccgc cgcaggactc gacgcgcatc
     1561 gcgatcgacg ccaggccgat gtggccttgc ccgagcgcct cttcggcgcg tccaggctca
     1621 attccacggc cgttgtcggc gacttcgaga agaatctgct cgcgggtgcg ccggaggctg
     1681 acggtcacct gggtcgcctc ggcatgctgg gccacgttgg acagcagctc gccgaccagc
     1741 gagacgatca gctggtcgta gacgccgctg gtttgcggct ggagactcaa cgtcacctct
     1801 aatccgccac gcttggcgtg aaggtcggcg accgccctga tcgccgcagc gatgccgccc
     1861 cgcgcacgtg tgacgggatg gagagcgacc acagcgctgc gcaggttgcc gatcgcttcg
     1921 acgacagcct cggctgcgtc tctgagcgcg tgatcgcctc cacttttgtc tcgtctcgcc
     1981 ttggccagat cttggcgggc agaaaggagg ctctgcagca cctcgtcgtg cagcgcctgc
     2041 gagatccgct cgcgcgcgcg atcctcggcg tcgagggcat ccgccatgat ccggcggcgc
     2101 tcatcggcca gcctggtcag ctgccgggtg agcttcacca tctcggcatt gcgctgccac
     2161 agctcctcct cggttttgcg gcgatcgatc gccagcgcca gcgtgttggc gaccgactcc
     2221 aagaaggtga cgtcgtctgc gctgaatgcg cgccccagcc ggctgtgtgc accaacgacc
     2281 ccccaaggtt ggctc
//
LOCUS       DASUBI010000304         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_675368, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000304 DASUBI010000000
VERSION     DASUBI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675368"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VFU72_05865"
     CDS             complement(<1..1461)
                     /locus_tag="VFU72_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423048.1"
                     /translation="MARAVQRQVDLVCLAAMELQHEEPDVSVVGQVHALLREITAQAE
                     AGAATPIVEGARLVTQAIAVADFESQAPTPYMGHFVTATAADLARVVHALLGELDPAP
                     ILSHARETLRLMPRRSTDFLVEIDLDDPEQVARIFAAVTEEEPELTVVQPIGQRNAEP
                     RRGQPARLRELRQLLATYVTHVEKLRAQPERRETLDDLLVGAKALRASAAAANLSEVN
                     RLLGRLVAVIDAQRASPEATTADMVAFALACARAIGEAIAAPDGSSEMFSALHEASSR
                     LLHHFQVDTGQFQTGILSGQPAGERRSLAQAPSASQPRAGGPEPGALDRVEQERLRQE
                     LAVALAHLPETIERLERDRHSVAARAALAQLIQLAHEAAVGAGLDALSVRCAALEQAL
                     EAAGFSTVPAEVLADARQLARELERQVRRADARPDDAAPRAAIPVDAAGLGEVVGLVQ
                     ELTLKRGQHGHEARSRLLGASVQEMAVFADRLRALAE"
     gene            1711..2520
                     /locus_tag="VFU72_05870"
     CDS             1711..2520
                     /locus_tag="VFU72_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5423049.1"
                     /translation="MSMGMGVGVEMEPPPVLSELRQGIGVVTLNRPRVLNALSRELAR
                     RLRQAIEELGANPALRVVVLRGAGRAFSAGADLTERLTLTPEETAGHTDLIREAVDAL
                     AALPVPVIAAVHGACLAGGAELALACDLRLAAEGASFGFPEVKRGIFPGAGAVARLPR
                     LVGPGQAADLIFTGRTIDAREALALGLIERLVRPNELGDAAAKIAAEIHANGPLAVRA
                     AKAALRQGLDRPLPEALALAEALRRPLDQTRDYREGLAAFAERRPPRFRGE"
BASE COUNT          324 a          950 c          940 g          335 t
ORIGIN      
        1 ctcggccagc gcccgcagcc ggtcggcaaa gacggccatc tcctgcacgc tggcgccgag
       61 cagccgcgag cgcgcctcgt gcccgtgctg cccacgcttc agcgtcagct cctgcaccag
      121 ccccacgacc tcgccgagac ccgccgcgtc caccgggatc gccgcgcgcg gcgccgcgtc
      181 gtccgggcgt gcgtccgcgc gccgcacctg ccgctccagc tcgcgcgcca actggcgcgc
      241 gtcggccagg acctcggccg gcacggtgga gaagccggcg gcttccagcg cctgctccag
      301 cgccgcgcag cgcacgctca gcgcgtccaa tccggcccca accgccgcct cgtgcgccag
      361 ttgaatcaac tgggccaacg cggcccgcgc cgcgaccgag tggcgatcgc gctccaggcg
      421 ctcgatcgtc tccggcagat gggccagcgc gaccgccagc tcctgccgca gccgctcctg
      481 ctcgacccgg tcgagcgcgc ccggctccgg gccgccggcg cgcggctggc tggcgctcgg
      541 cgcctgagcg agcgagcggc gctcgccggc cggttggccg ctcaggattc cggtctggaa
      601 ctggcccgtg tcgacctgga agtggtgcag caggcgcgag gaggcctcgt ggagggcgga
      661 gaacatctcg ctgctgccgt ccggcgcggc gatggcttcg ccgatggcgc gcgcgcaggc
      721 cagcgcgaag gcgaccatgt cggcggtggt ggcctcggga ctggcccgct gcgcgtcgat
      781 caccgcgacc agcctgccca gcagccggtt gacctcgctc agattggcgg cggcggccga
      841 ggcacgcagc gccttggcgc cgaccagcag gtcgtccagc gtctcacgcc gctcaggttg
      901 ggcgcgcagc ttctcgacgt gggtgacgta ggtggccagg agctggcgca gctcgcgcag
      961 gcgcgccggc tggccccggc gcggctccgc gttccgctgg ccaatgggct gcaccacggt
     1021 cagctccggc tcttcttcgg tgaccgcggc aaagatacga gccacctgct ccgggtcgtc
     1081 cagatcgatc tcgaccagga agtcggtgct gcggcggggc atcaggcgca acgtctcgcg
     1141 ggcgtggctg aggatcggcg cggggtccag ctcgcccagc agcgcgtgga cgacgcgcgc
     1201 caggtcggcg gcggtggcgg tgacgaagtg gcccatgtaa ggggtcggcg cctgactctc
     1261 gaagtcggcc accgcgatcg cctgggtgac gaggcgggcg ccctcgacga ttggcgtggc
     1321 ggcgccggcc tccgcctgcg cggtgatctc ccgcagcagg gcatgtacct ggccgacgac
     1381 ggacacgtca ggctcctcgt gctgcagctc catggccgca aggcagacga gatcaacctg
     1441 ccgctgaacc gcccgcgcca cctcggctat cgcctgcgat tgcactccgg gccccattcc
     1501 ggcagcttcg ctccggccgc gtcaccgcga cgctgtacgc caagcatagc ctgaccgcgg
     1561 gccgcgggct tgggatcgcg gtactgaaaa tcgcggcgcc cgacgggtag ggccggtgcc
     1621 tccgtaccgt gaccgcgagc gtccggcgag gcgctcgtat actgagacca gcggaacccg
     1681 cgcgtcgatc cgaacaatgg tggagtatgc atgagcatgg gcatgggtgt gggtgtagag
     1741 atggagccac cgccggtgct ctcggagctg cgccagggga tcggcgtggt cacgctgaac
     1801 cggccgcgcg tgctcaacgc gctgagccgg gaactggcgc gccggctgcg gcaggcgatc
     1861 gaggagctgg gcgcgaaccc ggccctgcgg gtcgtcgtgc tgcgcggtgc aggccgcgcc
     1921 ttctcggccg gggccgacct gaccgaacgt ctgacgctga ccccggagga gaccgccggg
     1981 cacaccgacc tgatccgtga ggcggtcgac gccctcgccg cgctgccggt gccggtcatc
     2041 gcggcggtgc acggcgcctg cctggcgggc ggcgccgagc tcgcgctcgc ctgcgacctg
     2101 cgcctcgccg ccgaaggcgc cagcttcggc ttccccgagg tcaagcgcgg catcttcccc
     2161 ggcgccggtg cggtggcgcg gctgccgcgc ctggtcgggc cgggccaggc cgccgacctg
     2221 atcttcaccg ggcgcaccat cgacgcgcgc gaggcgctgg cgctcggcct aatcgagcgg
     2281 ctggtgcggc cgaatgagct gggagatgcc gccgccaaga tcgcggcgga gatccacgcc
     2341 aacggcccgc tggccgtgcg cgccgccaaa gcggcgctcc ggcaggggct cgaccgcccg
     2401 ctgcccgagg cgctggcgct agccgaggcg ctgcgccgcc cgctcgacca gacgcgcgac
     2461 taccgcgagg gcctggcggc cttcgccgag cgccggcccc cacgctttcg tggggagtag
     2521 cagagcggag ccttcccttg caccgtgga
//
LOCUS       DASUBI010000305         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_17115, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000305 DASUBI010000000
VERSION     DASUBI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17115"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFU72_05875"
     CDS             <1..356
                     /locus_tag="VFU72_05875"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5423050.1"
                     /translation="YGDGQQTRSLCYVADLVRGLRLALETAGTTGEVINLGNPDERTV
                     VDLATIVLRLSDSRSELVYYPARPDDPYQRCPDIAKAERLLGWRPTIDIEQGMKETID
                     YFCAYVAPEPSTLGD"
     gene            346..1875
                     /locus_tag="VFU72_05880"
     CDS             346..1875
                     /locus_tag="VFU72_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5423051.1"
                     /translation="MAISRPAHLLTPSRAGVLLALLTAAVIAAPVYPINLVLIPAIAF
                     GIALLLHPMLAPVILIFSVPVQDVGGVMMGGASVTGTRLALVAVIAALALQLPTRGER
                     LRGTWLLAPFGLYLSAILLSLLGARQLSPGVDVFYQWGVTVFALVLTLYAVRTPRQAL
                     LLVAVMGAGAAAEAFLGLVQGLGNLGPASYAITSHVSRAFGTFGMPNSYAGYLEMTTP
                     LLAVVALWSLARTYADWLRYRQARVSGMAGSQSDRRRLVRGAALSAWLALAALLGFAG
                     IVMSFSRGAWLGFAAGGLAMLLVSGRRASLVAAAAIIFAGLVFAAGGVHYAPAAIQDR
                     YHQLTSQLTYFNSNDVLLTDENFAAVERMAHWQTGIAMFLAHPLSGVGAGNFNGRFAD
                     FAVNPAFTVSRGHAHNYYIQAAAETGSIGLISYLLLITGALLVCFRSAREAPTGLGRA
                     LGVGAVGVTVAVMVHNVVEDLHVLNLGIQLSAVWALAIIGWRFLPAGAGDAPRAEAGE
                     N"
     gene            1902..2909
                     /locus_tag="VFU72_05885"
     CDS             1902..2909
                     /locus_tag="VFU72_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5423052.1"
                     /translation="MLTTSAPEPVAEVSLARRVLKPRTLASFVIAAGVILFALSRFNL
                     DLGDVLEQMRGANPWYLLGAFAVYYGSFTFRAIRWRSLLGSADVRPEPGRHLPTLGGF
                     YVIYLLSWFVNCLVPAKLGDAYRGYALRQRSGASFSAALGTIFAERLADLVGLGVLLL
                     ASGFLVFGRHIPKSISDWLYVGVGLGMLLVVAVLVAYRIRHHLRALVPHRFRETYIRL
                     EEGALGSFSRLPTLIGLTAVIWLFEGLRFFLVGLSLHAGVSIGAALLVALLVSLLTAV
                     PITPAGLGIVELGTVGTLALLHVGQNTAASMAFLDRIVAYWSVLLIGGVVYLAARWRW
                     R"
     gene            2916..>3737
                     /locus_tag="VFU72_05890"
     CDS             2916..>3737
                     /locus_tag="VFU72_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423053.1"
                     /translation="MRVAIDYTSALRQRGGVGRYTRDLVAALLAEAPEDEFLLWHAAD
                     CSSGIPQAANVLPCRLPISTRWLTAGWQRLRLPITLERFVGPVDVAHAPDFVAPPSHA
                     PAIATIHDLSYLITPEYAHPALRRFLAAAVPRTLARAARVLAVSETTRVDLIERYGLN
                     PGRVVSVPNGVDRRFRTPGPEEMDNALRLFDLRRPYFLIVGTIEPRKNHLALLRAFER
                     VSQRWPEAALVIIGQPGWLAEPIVARIEDAAQRLPVRYLRFVDDRWLPSLYAASTA"
BASE COUNT          470 a         1338 c         1205 g          724 t
ORIGIN      
        1 tctacggcga cggtcagcag acacgctcgc tctgctacgt ggcggatctc gtgcgcggcc
       61 tgcgcctcgc ccttgagaca gccgggacaa ccggcgaggt gatcaatctg ggcaatcccg
      121 acgagcgcac ggtcgtggat ctggccacca tcgtgctgcg tttgtctgat tcccgctcag
      181 agctggtcta ctacccggcc cggccggacg acccctacca gcgctgtccg gatatcgcca
      241 aggcagagcg cttgctcggc tggcggccaa cgatcgacat cgagcagggc atgaaggaga
      301 cgatcgacta tttctgcgcc tacgtcgcgc ccgagccatc cactcttggc gattagccgc
      361 ccagcgcacc tgctcacacc ctcgcgcgcc ggtgtgctgc tggcgctgct gacggccgcc
      421 gtcatcgccg ctcccgtcta tccaatcaac cttgtgttga tcccggcaat cgcgtttggt
      481 atcgcactcc tgctccaccc aatgttggcg ccggtgatcc tgatcttctc cgtgccggtg
      541 caggacgtcg gaggggtcat gatgggcggc gcgtcggtga ccggcacccg gctggcgctg
      601 gtggccgtga tcgcggcgct cgccttgcag ctccccactc gcggggaacg gttgcgcggc
      661 acctggctgc tggcgccctt cggtctctac ctgagcgcca ttctcctctc gctcctcggg
      721 gccaggcagc tgtcccccgg cgtcgacgtc ttctaccaat ggggcgttac ggtcttcgcg
      781 ctcgtgctga ccctctacgc cgtgcgcacg ccgcgccagg cgttgctctt ggtcgcggtg
      841 atgggcgccg gcgcggcggc ggaggcgttc cttggcctgg tgcaaggctt gggcaatctc
      901 ggcccggcca gctacgccat cacgtcgcat gtctcacgcg cgtttggcac cttcggtatg
      961 ccaaacagct acgccggcta cctggaaatg accacgcccc tgttagcggt ggtggcgctc
     1021 tggtcactgg cgcggacata cgccgactgg cttcgctacc ggcaggcgcg ggtgtccggg
     1081 atggcgggca gccagtcaga ccgacgacgg ctcgtgcgcg gcgccgcgct cagcgcctgg
     1141 ctggcgctgg ccgcgctgct gggctttgcc ggcatcgtca tgagcttctc gcgcggcgcg
     1201 tggctcggct tcgcggccgg cgggctggcc atgttgctcg tctccggccg tcgtgcttcg
     1261 ctggtcgcgg cggcggcgat catcttcgcc gggctggtgt tcgcggccgg cggcgttcac
     1321 tatgcgccgg ccgccattca ggatcgttat caccagttga ccagccagtt gacttatttc
     1381 aactcgaacg acgtcctgct cacggacgag aatttcgccg ccgtcgagcg catggcccat
     1441 tggcagaccg gcatcgcgat gttcctggcg cacccgctca gcggggttgg agcgggcaac
     1501 ttcaatggcc gcttcgccga cttcgcggtc aacccggctt tcaccgtctc gcgcgggcac
     1561 gcccataact actatatcca ggccgccgcg gagaccggct ccatcggtct gatcagctac
     1621 ctgctgctca tcaccggcgc gctcctggta tgcttccggt ctgcgaggga ggcaccaacc
     1681 ggcctgggcc gagcgctcgg cgtgggtgcg gtaggcgtca cggtggcggt gatggtgcac
     1741 aacgtcgtcg aagacctgca cgtgctcaat ctcgggatcc agctctcggc ggtctgggcg
     1801 ctcgccatca tcggctggcg cttcctccca gcgggggctg gcgacgctcc acgagcggag
     1861 gcgggcgaaa actgatggtt ggacgtcctg tggaagaacc gatgctcacg acgagcgcgc
     1921 ccgaaccggt ggccgaggtt tcgttagccc gccgggtgct gaagccgcgc acgctggcct
     1981 cgttcgtgat cgcggccggc gttatcctct ttgcgctctc gcgcttcaat ctcgacctgg
     2041 gtgacgtgct tgaacagatg cgcggcgcca acccctggta cctgctcggc gccttcgccg
     2101 tctactacgg ttccttcacc ttccgcgcga tccgctggcg ctcgctgctc ggcagcgccg
     2161 atgtacgccc cgaaccgggc cggcacttgc ccaccctggg cggcttctac gtcatctacc
     2221 ttctctcctg gttcgtcaac tgtctggtgc cggccaagct cggcgacgcc taccgcggct
     2281 acgccctgcg ccagcgctcc ggagcatcct tcagcgccgc gctgggcacc atcttcgccg
     2341 agcggctcgc cgatctggtg gggcttggtg tgctgctgct ggcctcgggc ttcctggtct
     2401 tcgggcgcca catccccaag agcatctcgg actggctcta cgtcggcgtt ggcctcggga
     2461 tgctcctggt cgtcgcggtg ctggtcgcct atcgcatccg gcaccacttg cgcgcactgg
     2521 tgccgcaccg cttccgcgag acctatatcc gactggagga gggcgccctc ggctccttct
     2581 cgcggctacc gacgttgatc ggcctgacag cggtcatctg gctcttcgaa ggtctgcgct
     2641 tctttctcgt tgggctatcg ctgcacgccg gcgtctcgat cggcgcggcg ctgctggtgg
     2701 cgctgctggt cagcttgctg acggccgttc cgattactcc cgccgggctg ggcatcgtcg
     2761 agctggggac cgtcggcacc ctggcgctcc tccacgtcgg acagaacacc gccgcctcga
     2821 tggcattcct cgatcgcatc gtcgcctatt ggagcgtcct gctgatcggc ggcgtcgtct
     2881 acctcgcggc gcgctggcgc tggcggtaga cgccgatgcg cgtcgcgatc gactacacct
     2941 ccgcgctgcg tcagcggggc ggcgtcggac gctacacgcg cgacctggtc gcggcgctgc
     3001 tcgccgaagc tccggaggac gagttcctgc tctggcacgc cgccgattgc tcatccggga
     3061 ttccacaggc ggcgaacgtt cttccctgcc ggctgcccat ctcgacgcgt tggctgactg
     3121 ccggctggca gcggctccgg ctgccgatca cgctcgagcg ctttgtcggc ccggtggatg
     3181 tcgcgcacgc gcccgacttc gtggccccgc cgagccacgc gcccgccatc gcgaccatcc
     3241 acgacctctc gtatctgatc acgcctgagt acgctcaccc cgctttgcgc cgcttcctgg
     3301 cggcggccgt gccgcgcacc ctggcgcgag ccgcgcgcgt gctggccgtc tcggagacaa
     3361 cccgcgtcga tcttatcgag cgctacggcc tcaacccggg ccgagttgtc tcggtcccaa
     3421 acggagtcga ccgccgcttt cgaactccag gcccggagga gatggacaac gcgctacgcc
     3481 tctttgacct acgccggccc tacttcttga tcgtcggcac catcgagccg cgcaaaaacc
     3541 acctggccct gctgcgcgca ttcgagcgcg tgagccagcg ctggcccgag gccgcgctgg
     3601 tgatcatcgg gcagcccggc tggctggccg agccgatcgt ggcgcgcatc gaggatgccg
     3661 cgcaacggct tcccgtccgc tacctgcgtt tcgtcgacga tcgctggctg ccgtcgctct
     3721 atgcggccag cacggcc
//
LOCUS       DASUBI010000306         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_550151, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000306 DASUBI010000000
VERSION     DASUBI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550151"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..2225)
                     /locus_tag="VFU72_05895"
     CDS             complement(390..2225)
                     /locus_tag="VFU72_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEU5423054.1"
                     /translation="MRSRRSRQRPSEPSREQPELAWRKMDLHIHTPASSDYQQPQVTF
                     IDILRRAEEVGLDILAFTDHNSVRGFARMWREIEDLELLEALGRISPSEAELLAEYRR
                     LLNRILLLPGFEFTATFGFHILAIFPPETSIRKLEQTLLALNIAEEKLDQGTSEVGAT
                     TEVREAYELMHGAGAMVIPAHVNSTHGVAMQGFPFGGQTKIAYTQSEEIHALEATDLE
                     SGGSRSTARFFNGSKTEYPRRMHVIQGSDAHRLLRESERSKDLGVGDRVTEVALPVLS
                     FEALRAVFEGNDFSVVRPFTGSEGKLAQALGAREQGATQTQAFHASLGKSRRKTNTLF
                     KDVVAFANGEGGTIYVGMDATAGGAVTGVPRAKVMAEQVREDLARFIAPAPVATVEVL
                     SAHDHDLLTVTIASGPDKPYAIADGEIYIREGGESLVANRDQIVELVRRALLPAATAQ
                     PEPIEPEAEPEPLAVLAERTASDEVPLPRSGFEVLGAVERDGATHYTLLDLRERKLLE
                     DVTAENARRLESYAIRQSQSGTPERAAVKWEGPFGVIRRRKERNGNVRYDLAYQHNGD
                     LRVFYNVAAGSLGKEWRAAVQALSPTTSGGNGNGNGNGNGAGAGR"
     gene            2435..>2697
                     /locus_tag="VFU72_05900"
     CDS             2435..>2697
                     /locus_tag="VFU72_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5423055.1"
                     /translation="MSVFVVLDILLLILIALPIPIGFWRGAQREAFVTLGILFGAALS
                     DWWARPWGGDLAAMSGLRDSAGAFMVAMLFLVGATFLLGYGTGA"
BASE COUNT          387 a          956 c          881 g          473 t
ORIGIN      
        1 gttcggagac gccgggcccg cgcgcccccg cttgcgcccg agcaccgccc cggcgatcgc
       61 ccgccccggt ccctgctggt agccccgcag cgcccgcttc gggaggaaca cccgcgagaa
      121 ctcccacggc tcggccagcg ccgcccgcag ccccccaccc cgtcccatct ctcccccctc
      181 cccgcattga gtaggaccat caaagcgggc cacagcctga caggggaaac tcggaccagg
      241 atcaagaaga gcgccgccgt tgattttccg cacgggcagc acctccggcg ctgcccctcg
      301 cccattgccc caagcagcgc aaaacaaaac ccctcgccca aaggtgggcg aggggtttct
      361 gtgtctagac cgtggcaggc gccgtcaggt tagcgcccgg cgccggctcc gttgccatta
      421 ccgttgccgt tgccgttgcc gccgctggtg gtcgggctga gcgcttgcac cgcggcacgc
      481 cactccttgc ccaggctgcc cgcggccaca ttgtagaaga cgcgcaagtc gccgttgtgc
      541 tggtaggcca ggtcgtagcg cacgttgccg ttgcgctcct tgcggcgccg gatgaccccg
      601 aaggggcctt cccacttgac cgccgcgcgc tccggcgtgc cggattggct ttgccggatg
      661 gcgtagctct ccagccggcg cgcgttctcg gcggtcacgt cttccagcag cttgcgctcg
      721 cgcaggtcga gcaacgtgta atgggtggct ccgtcgcgct cgacggcgcc cagcacctcg
      781 aagccgctgc gcggtagcgg cacctcgtcg ctcgccgtac gctcggccag cacggccagc
      841 ggctccggct cggcctcggg ttcgattggc tccggctgcg cggtcgcggc cggtagcagc
      901 gcgcgccgca ccagctcgac gatctgatcg cggttggcga ccagcgactc gccgccctcg
      961 cggatgtaga tctcgccgtc ggcgatcgcg tagggcttgt cgggaccgct ggcgatcgtg
     1021 accgtcagca gatcgtggtc gtgggcgctc aatacctcga ccgtggctac cggggccggc
     1081 gcgatgaagc gcgcgagatc ctcgcgcacc tgctcggcca tgaccttggc tcgcggcacg
     1141 ccggtgacgg cgccaccggc ggtcgcatcc atgcccacgt agatcgtgcc gccctcgccg
     1201 ttggcgaagg ccaccacgtc cttgaagagg gtgttggtct tgcgccggct cttgcccagg
     1261 ctggcgtgaa atgcctgcgt ctgggttgca ccctgttcgc gggcgccgag ggcctgcgcc
     1321 agcttgccct ccgagccggt gaatggccgc actaccgaga agtcgttgcc ctcgaaaacc
     1381 gcgcgcagcg cctcgaagct caacaccggc agcgccacct cggtcacacg gtcgccgacg
     1441 cccaagtcct tggagcgctc ggactcgcgc agcagccggt gcgcgtcgga accctgaatc
     1501 acatgcatcc ggcgcggata ctcggtcttg ctaccgttga agaagcgcgc ggtcgagcgc
     1561 gagccgccgc tctccagatc ggtggcctcc agcgcgtgga tctcctcgct ctgggtatag
     1621 gcgatcttgg tctgtccgcc gaagggaaaa ccctgcatcg ccaccccgtg cgtcgagttg
     1681 acatgggccg ggatcaccat ggcccctgcc ccgtgcatca gctcgtaggc ctcacggacc
     1741 tccgtcgtgg cgccgacctc gctggtgccc tgatcgagct tctcctcggc gatgttcagc
     1801 gccaggagcg tctgctccag cttgcggatc gacgtctcgg gcgggaagat cgccaggata
     1861 tggaaaccga aggtcgcggt gaactcaaag ccggggagga ggaggatgcg gttgaggagg
     1921 cgacggtact cggccagcaa ctcggcctcg cttgggctga tgcgtcccag cgcttcgagg
     1981 agctccaggt cctcgatctc gcgccacatg cgggcgaagc cgcggacgct gttgtggtcg
     2041 gtgaatgcca gaatgtcgag cccgacttcc tcggcgcggc gcaggatgtc gatgaaggtc
     2101 acctgcggct gctgatagtc gctcgaggcg ggcgtatgaa tgtgcaagtc catcttgcgc
     2161 caggcgagtt ctggctgctc gcgcgacggc tcggacggac gttggcggga acgtctgctt
     2221 ctcaccaagg gttctcctgt tcaccacctt tctgtgacat agaacgagcc gtggcggtcg
     2281 aattcgcagc cacactcatt ctatgcgcaa ttctaccata actggaggga cgaaccggag
     2341 cacggcgggc gccagcggtc ctgatccatg ttagaatacc ggccaccctg ccgcatcggg
     2401 gccacgcgcc ggccgcgctg atgagggaga gcacgtgagc gtcttcgtcg tcctcgacat
     2461 tctcctcttg attctgatcg ctttgccgat cccgatcggc ttctggcgcg gtgcccagcg
     2521 cgaggcgttt gtgacgctcg gcatcctctt cggcgccgcc ctctccgact ggtgggcgcg
     2581 cccctggggc ggcgacctgg ccgccatgag cggcctgcgc gactccgccg gcgccttcat
     2641 ggtcgccatg ctcttcctgg tcggcgccac cttcctgctc ggttacggca ccggcgc
//
LOCUS       DASUBI010000307         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_71163, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000307 DASUBI010000000
VERSION     DASUBI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71163"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFU72_05905"
     CDS             <1..705
                     /locus_tag="VFU72_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423056.1"
                     /translation="GSVERIKELLAAGFPVITPQWLDHKPDAIGHYRVVRGYDDARGG
                     FLVNDSMIGPDVFYSYDDFVWLWRAFNYRYLPVYRPADEPKVLAILGPDADHQQNLLR
                     ALDLFTGLAAQNPNDAYLRFSIGTSFYELGNKQEAVAAYERAAQLGLPPKMLWYQFWP
                     VMAYNDVGNYHAALELATVQIADADTFGEMRYERGRAFEALGNLPAAIADYHRAVTDD
                     ANLTAPHEALKRLGLE"
     gene            715..2316
                     /locus_tag="VFU72_05910"
     CDS             715..2316
                     /locus_tag="VFU72_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5423057.1"
                     /translation="MSKPLVFVNGHVMTAEPEPRPASAVAVHNGSIVAVGSDDEMRPW
                     RDSVGAELIDLHGRTLTPGFNDAHCHPMGVGFSLLMVNAATPPHRRIADIMAAIGERA
                     AQSADGAWVVARGYDDARLAENRHPTRQDLDAISPNNPVVLIRACGHIGVANSRALVL
                     AGIGRDTANPEGGTIDRDAHGEPTGVLREEALGLVRDLIAEPTANQIQEALLLAGKKY
                     RSEGVTSWAEAGIRTAAELQAYEGLAQAGKLPLRSYLMMMIAETLEPLSRLGIHTGFG
                     DPWLRIGPAKLFLDGSIGGRTARMSQPYEGQDDNLGLWMEEPRVMREKLIAAHRAGFQ
                     CCAHAIGDAAIELLIQTYEEAQQRYPRPNARHRIEHSSILRPDLIDRIVKLGAIPVPG
                     TSFLFAFEKAYVQNLGYDRIRWAYAMRTFFDRGIVAPASTDAPVVSTNPMIGIQTMMT
                     RRSEEGDVIWPEEAVSLEEAVRAYTYNGAYASFEDEIKGTVEPGKLGDLTVWETDLRT
                     VAPGEMAGVRADLTVIDGEVVYERA"
     gene            2365..3060
                     /locus_tag="VFU72_05915"
     CDS             2365..3060
                     /locus_tag="VFU72_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5423058.1"
                     /translation="MLTIRYLGHSAFSLDNGEHEVLIDPFLSGNPTAVTSHEEHTPIA
                     ILLTHGHNDHVGDAVAIAKREDAPIITTYELANYLETEGAKTIGGGHGGTIKFQGGSA
                     KFVPAWHSSSYTNAQGNVVAPGVPAGFVVRFGGKTVYFAGDTCLFGDMKLIGEEGLDG
                     AVLPIGDHFTMGPQDALKAVKLLEPKVAFPCHYNTFEAIQQDEKAWGKKVESETDTKV
                     VVLAPGESYKLTE"
     gene            3198..3560
                     /locus_tag="VFU72_05920"
     CDS             3198..3560
                     /locus_tag="VFU72_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423059.1"
                     /translation="MSMDATPTPEERRAALTAEMSRTAFQRKAVSRAAPRAALQEAID
                     FFTARGYRAGWTGRPNQVFVMGGREGILPRVNAEILAQANVGKGKVTMLTISGFGEDL
                     SRHLAAYVDHLRQQRKSS"
     gene            complement(3601..>4538)
                     /locus_tag="VFU72_05925"
     CDS             complement(3601..>4538)
                     /locus_tag="VFU72_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEU5423060.1"
                     /translation="LEACVDEGLEFTILSPEQAAAPADSRHPYQVTLPSGRDFGVVFY
                     EGGLSGTVSFDPIATNSSREFLERFVLPRFDADDPDDPQTITIASDGEVYGHHHRFKD
                     YFLQDLLYARAEEAGLQVVSSEQLLALPQPRRETAIRERSSWGCAHQLLRWSGDCDCT
                     PGSGQWKADLRRALDRLAARVDDLTERAGGAFDIWALRDDYVDVIMGAVTLTGWLAAH
                     GIEPESDDAGRVTRLLEAQRHRLAMYASCAFYWDDLTRIEAGYGVRSALYAASMLDQS
                     FGSDLAAGFTADLDSVVGWKSDRSAAQLYASSAAR"
BASE COUNT          794 a         1552 c         1528 g          664 t
ORIGIN      
        1 ggcagcgtcg agcgcatcaa ggaactgctg gccgctggct tcccggtcat caccccacag
       61 tggctcgacc acaagcccga cgccatcggc cactaccgcg tggtgcgtgg ctacgacgac
      121 gcgcgcggcg gcttcctggt caacgactca atgatcggtc ccgatgtctt ctacagctac
      181 gacgatttcg tctggctgtg gcgcgccttc aattaccgtt acctgccggt ctatcgcccc
      241 gccgacgagc ccaaggtgct cgccatcctg gggccggatg ccgaccacca gcagaacctg
      301 ctgcgcgccc tcgatctgtt caccgggctc gccgcgcaga acccgaacga cgcctacctg
      361 cgcttctcga tcggcaccag cttctacgag ctgggcaaca agcaggaggc ggtggccgcc
      421 tacgagcggg ccgcccagct cggtctgccg cccaagatgc tctggtatca attctggccc
      481 gtgatggcgt ataacgatgt ggggaactat catgcggccc tggagttggc gaccgtgcag
      541 atcgcggacg ccgacacgtt cggcgagatg cgctacgagc gcgggcgggc ctttgaagcg
      601 ctgggcaatc tccccgccgc catcgcggat taccaccgcg cggtgaccga cgacgctaac
      661 ctgaccgcgc cccacgaggc gctcaagcga ctgggccttg agtagaggag aagcatgagc
      721 aagccgctgg tcttcgtcaa cgggcacgtc atgaccgcgg agcccgagcc gcgccccgcc
      781 agcgcggtgg cggtgcacaa cggcagcatc gtggcggtcg ggtcggacga cgagatgcgg
      841 ccctggcgcg acagcgtggg cgccgagctg atcgatcttc acgggcgcac gttgactccc
      901 ggcttcaacg acgcgcactg ccacccgatg ggcgtcggct tcagcttgct gatggtcaac
      961 gcggccaccc cgccgcaccg gcgcatcgcc gacatcatgg cggccatcgg cgagcgtgcg
     1021 gcgcagtcag cggacggcgc ctgggtcgtg gcgcgcggct atgacgacgc gcggctggcc
     1081 gagaaccgcc acccgacacg ccaggatctc gacgcgattt cgcccaacaa tccggtcgtc
     1141 ctgatccgcg cctgcggcca catcggcgtc gccaacagcc gcgcgcttgt tctggccggc
     1201 atcggccgcg acaccgcgaa cccggagggc ggcaccatcg accgcgacgc gcatggcgag
     1261 ccgaccggcg tgttgcgcga agaggcgctc ggactggtgc gcgatttgat cgcggagccg
     1321 acggcgaacc agattcaaga ggcgctgctg ctggcgggca aaaaataccg ctcagagggt
     1381 gtcacgagtt gggccgaggc gggcatccgc accgccgccg agttgcaggc ctacgagggg
     1441 ctggcgcagg cgggcaagct gccgctgcgt agctacctca tgatgatgat cgccgagacg
     1501 ctggagccgc tcagccggct cggcatccac accggcttcg gcgacccttg gctgcgcatc
     1561 ggcccggcca agctcttcct cgacggcagc atcggcgggc gtaccgcgcg catgtcgcag
     1621 ccctacgagg gccaggacga caatctcggc ctctggatgg aggagccgcg cgtgatgcgg
     1681 gagaagctga tcgcggccca ccgcgccggc ttccagtgct gcgcccacgc catcggcgac
     1741 gccgccatcg agctgctgat ccagacctac gaagaggcgc agcagcgcta tccgcgcccc
     1801 aatgcgcgcc accgcatcga gcacagcagc atcctgcggc ccgacctgat cgatcgcatc
     1861 gtgaagctcg gcgccatccc ggtccccggc accagcttcc tcttcgcctt cgagaaggcc
     1921 tacgtacaga acctgggcta cgaccgcatc cgctgggcct acgccatgcg caccttcttc
     1981 gaccggggca tcgtcgcgcc ggccagcacc gacgcgcccg tcgtctccac caacccgatg
     2041 atcggtatcc agacgatgat gacgcgccgc agtgaggagg gcgacgtcat ctggcccgag
     2101 gaggcggtca gcctggagga ggccgtgcgc gcctacacct acaacggcgc ctacgcctcg
     2161 ttcgaagatg agatcaaggg cacggtcgag ccgggcaagc tgggcgactt gacggtctgg
     2221 gagactgatc tacgcacggt ggcgcccggg gaaatggcgg gcgtgcgcgc cgatctcacc
     2281 gtgatcgatg gtgaggtcgt ctacgaacgc gcgtgaatcc gatcggcggt gtgatcggac
     2341 gtgtcgacag cgaaggaggg atccatgctg accattcgct acctggggca ctcggcgttc
     2401 tcgctggaca acggcgagca cgaggttctg atcgacccct tcctgagcgg caatccgacc
     2461 gccgtcacca gccatgaaga gcacaccccg atcgcgatcc tgctcaccca cggccacaac
     2521 gaccacgtcg gggacgccgt cgccatcgcc aaacgcgagg acgcgccgat catcaccacc
     2581 tatgagctgg cgaactatct ggagaccgag ggcgccaaaa cgatcggcgg cggccatggt
     2641 ggcacgatca agttccaagg cggcagcgcc aagttcgtgc cggcctggca ctcttcgtcc
     2701 tataccaacg cgcaggggaa tgtggtcgcg ccgggcgtgc cggccggctt cgtggtgcgc
     2761 ttcggcggca agacggttta cttcgccggc gacacctgcc tcttcggcga catgaagctg
     2821 atcggcgagg agggcctcga cggcgcggtg ctcccgatcg gcgaccactt caccatgggc
     2881 ccgcaggacg cgctgaaggc cgtcaagctg ctggagccga aggtcgcctt cccctgccac
     2941 tacaacacct tcgaggccat ccagcaggac gagaaggcct ggggcaagaa ggtcgagtcc
     3001 gagaccgaca ccaaggtggt cgtgctggcc cccggcgagt cgtacaagct gacagagtaa
     3061 cggaaggact ggccgtcgac gaggctggtt gccacgaagg gcaggctctg agcgccgtcg
     3121 aaggatccgg gcctccgccg gcactcaaac gaagtcggta ggcagccctc gaatgcccgg
     3181 ccgcggcagg aaagcccatg tcaatggacg cgacaccgac accggaggaa cgccgggcgg
     3241 ccttgaccgc cgagatgagc cgcaccgcct ttcaacgcaa ggcggtctcg cgtgccgccc
     3301 cgcgcgccgc gctgcaagag gcgatcgatt tcttcacggc gcgcggctac cgcgccggct
     3361 ggacgggacg gcccaaccag gtcttcgtca tgggcgggcg cgagggcatc ctgccgcgcg
     3421 tcaacgccga gatcctggcc caggccaacg tcggcaaggg caaggtcacc atgctcacca
     3481 tcagcggttt cggggaagat ctcagccggc acctggccgc ctacgtcgat cacctgcgtc
     3541 agcagcgcaa gtcaagctga agtcccaaac tgagatgccc ctggttgagc gcggtcgtgc
     3601 tcagcgcgcg gcggaggagg cgtacagctg ggcggcgctg cggtccgact tccagccgac
     3661 gaccgagtcg aggtcggcgg tgaaaccggc ggccaggtcg ctgccgaacg actggtcgag
     3721 catactggcg gcgtagagtg ccgagcgcac tccgtagccg gcttcgatgc gcgtcaagtc
     3781 atcccagtag aaggcgcaac tggcgtacat cgccagccgg tgacgctggg cctccagcag
     3841 ccgggtaacg cggcccgcgt cgtcgctctc cggctcgatc ccgtgcgccg cgagccagcc
     3901 ggtcagcgtc accgcgccca tgatgacgtc gacgtagtcg tcgcgcaggg cccagatgtc
     3961 gaacgcgccg cccgcgcgct cggtgaggtc gtcgactcgc gccgccagcc tatcgagcgc
     4021 gcgccgcaag tcggccttcc actggcccga gccgggggtg cagtcgcagt cgccgctcca
     4081 gcgaagaagc tgatgggcgc agccccacga cgaacgttca cggatcgcgg tctcgcgccg
     4141 cggttggggc agcgccagga gctgctcgct ggagacaacc tgcagcccgg cctcttcggc
     4201 gcgggcatag agcaggtctt gcaggaagta gtccttgaag cggtggtggt ggccgtacac
     4261 ctcgccgtcg ctggcgatgg tgatcgtctg tgggtcgtcc ggatcatcgg cgtcaaagcg
     4321 cggcagcacg aagcgctcga ggaattcccg cgaggagttg gtagcgatgg ggtcgaagga
     4381 gaccgtcccc gagagcccgc cttcgtagaa gacgacgccg aaatcgcgcc ccgacgggag
     4441 cgtgacctgg tagggatggc gactgtcagc cggcgcggcg gcctgctccg ggctgaggat
     4501 ggtgaactcc agcccctcgt cgacgcaggc ctctaagg
//
LOCUS       DASUBI010000308         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_624557, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000308 DASUBI010000000
VERSION     DASUBI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624557"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFU72_05930"
     CDS             complement(<1..301)
                     /locus_tag="VFU72_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423061.1"
                     /translation="MATAETGERLTRRDFFHDLAGELGPLLPESLRGFQSHATHNLLK
                     LSYGERRLHFEVWANARDSHIEIGLHFEDGPESTERLLAFFNRHIVELKHELGPEL"
     gene            complement(317..1132)
                     /locus_tag="VFU72_05935"
     CDS             complement(317..1132)
                     /locus_tag="VFU72_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEU5423062.1"
                     /translation="MSQSSRTSYHTHSRYCDGHGEIAEVIEEAIAAGLETVGISSHAP
                     LPFSTEWNMPLERLAGYVAEVRGLQTRYADQIRVLLGSEIDYIPGDEVAEFQRREVHP
                     LGFDYYVGSVHFLGSGSPPRSFDGTEADFRAILRDGYGDDISRMVADYYRRLPGVLTI
                     PGVRVVGHFDVIKRWNAGQKYFRGDEPWYLDAVEGALQPIAASGQIVELNTAGWRKGL
                     GEPYPSPAILARCRELGIPITVNSDSHTPAEVAAGFRQAAELLAGLGIEPVSL"
     gene            complement(1158..>3196)
                     /locus_tag="VFU72_05940"
     CDS             complement(1158..>3196)
                     /locus_tag="VFU72_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEU5423063.1"
                     /translation="RWLQELFPVDELLAAGLGLPLERIALEEWSGDELYAVDAYDTEG
                     QRLLEETFSPLSYRRPYLGLYPAAGTVHVVGGGLIARQPGRELIEPVPTDLDLFWSYY
                     QGEVLPRVARFILESTGGEPRVVDQPFFDELRVDLSLSATDEPFGVREERRSATEALH
                     EDIYFNTLDLIETLGQELSGEPFAAPGAVLPFVHLRPGKAPTARISLRARPRAVAELE
                     LDGALEPLGAIADQLPPEGAVTGYTLDRDGTAGLQVALALDVQQQARLAALAALFPVA
                     AGEPALTVSAGSETVALAWPTAPPPPEAVPAERATPEGEIMNAERLSRQLARLAAAPE
                     ARYTPAVEWSYGGRALPLLELTAPITTSLWSPRKLSLLKPTLLIIARHHANEVASTTA
                     ALLLAERLVDLPEWRWLLSRVNLVVLPLANPDGAALHDLLQREHPTWKHHPARYNATG
                     FEFAADIGNPDSRYGEARARDKVFRRWLPDIVVDNHGVPSHEWAQLYAGFGSPPRFGV
                     SYWQVQALIYGIIHYDEERPAHRQAAFALRNAVAAAVAADAELLAWNRRFRERYQTWG
                     ANRLPDRFPAETHREMVFHFGPASGRRGRGGRDFAARFPGLTVADWVTEVADETVRGA
                     MLRRTAGAHLTANRATLDLLAAAATPPQRRILSSADGARTRITLCRRRPILLGG"
BASE COUNT          454 a         1128 c         1162 g          452 t
ORIGIN      
        1 ccagctccgg cccgagctcg tgcttcagct ccacgatgtg ccggttgaag aaggccagca
       61 ggcgctcggt gctctccggt ccgtcctcga agtgcagccc aatctcgatg tggctgtcgc
      121 gcgcgttggc ccagacctcg aagtgcagcc gccgctcgcc gtaggagagc ttcagcaggt
      181 tgtgggtggc gtggctctgg aagccgcgca acgactccgg cagcagcggc cccagctcgc
      241 cggccaggtc gtggaagaag tcgcgccgcg tcagccgctc gccggtctcc gccgtcgcca
      301 tcgtctccct ccgggcttac aggctcaccg gctcgatccc cagcccggcc agcagctcgg
      361 ctgcctgccg gaagccggcc gcgacctccg ccggcgtgtg cgagtcgctg ttgaccgtga
      421 tcgggatgcc cagctcgcgg cagcgcgcca ggatggccgg cgagggatag ggctcgccca
      481 ggcccttgcg ccagccggcg gtgttcagtt cgacgatctg acccgaggcg gcgatgggct
      541 gcagcgcacc ctcgaccgcg tccaggtacc agggctcgtc accccggaag tacttctgcc
      601 cggcgttcca gcgcttgatc acgtcgaagt gccccaccac gcgcacgccg gggatggtga
      661 gcacgccggg caggcggcga tagtagtccg ccaccatgcg cgagatgtcg tcgccgtagc
      721 cgtcgcgcag gatggcgcgg aagtcggcct cggtgccgtc gaaggagcgc ggcggtgagc
      781 cactgcccag gaagtggacc gagccgacgt agtagtcgaa gccgagcggg tggacttcgc
      841 gccgctggaa ctccgctacc tcatcgccgg ggatgtagtc gatctcggag ccgagcagca
      901 cccggatctg gtcggcgtag cgcgtctgga ggccgcgcac ctcggccacg tagccggcca
      961 gccgctccag cggcatgttc cactccgtgg agaaggggag cggagcgtgg ctggagatgc
     1021 cgaccgtctc gagcccggcg gcgatggcct cctcgatcac ctcggcgatc tcgccgtgac
     1081 cgtcgcagta gcgactgtgc gtgtggtagc tggtgcggct cgactggctc atctcaccct
     1141 ctcctgaagc tcgcggctca cccgcccagc aggatcggcc gccgccggca cagcgtgatc
     1201 cgcgtacgcg cgccgtccgc gctgctaagg atacgtcgct gcggcggggt ggcggcggcg
     1261 gccagcagat cgagcgtggc ccgattggcg gtcaggtgcg cgcccgcggt gcgacgcagc
     1321 atcgccccgc gcaccgtctc gtcggctacc tcggtcaccc aatcagccac cgtcaggccg
     1381 gggaaacgcg cggcgaagtc gcgcccacct cggccgcggc ggccggaggc cgggccgaaa
     1441 tggaagacca tctcgcggtg tgtctcggcc gggaagcgat ccggcaggcg gttggcgccc
     1501 caggtctggt agcgctcacg gaagcggcgg ttccaggcca gcagctccgc gtcggcggcg
     1561 accgccgcgg cgacggcgtt gcgcagggcg aaggccgcct ggcggtgagc cggccgctcc
     1621 tcgtcgtagt ggatgatgcc gtagatcagc gcctgcacct gccagtagct gacgccaaag
     1681 cgcggcggcg agccgaagcc ggcgtagagc tgcgcccact cgtgcgaggg tacgccgtgg
     1741 ttgtcgacca cgatatcagg cagccagcgc cgaaacacct tgtcgcgcgc ccgcgcctcg
     1801 ccgtagcgcg agtcggggtt accgatgtcg gcggcaaact cgaagccggt ggcgttgtag
     1861 cgcgccggat ggtgcttcca ggtcgggtgc tctcgctgca gcaggtcgtg cagcgcggcg
     1921 ccgtcggggt tggccagcgg cagcacgacc aggttgaccc ggctgagcag ccagcgccac
     1981 tccggcaggt ccaccaggcg ctcggccagg agcagcgcag cggtcgtgct ggcgacctcg
     2041 ttggcgtggt ggcgcgcgat gatcagcagg gtgggtttga gcagcgagag cttgcgcggc
     2101 gaccagaggc tggtcgtgat cggcgccgtc agctcgagca gcggcagcgc ccgcccgccg
     2161 taggaccact cgacggccgg ggtgtagcgc gcctccggcg ccgccgccag ccgggcaagc
     2221 tgccggctga ggcgttcggc gttcatgatc tcgccctccg gcgtggcccg ctcggccggt
     2281 acggcttcgg gcgggggagg agcggtcggc caggccagcg cgacggtctc cgacccggcc
     2341 gacacggtca gcgccggctc gccggcggcc accgggaaga gcgcggccag cgcggccagg
     2401 cgcgcctgct gctggacatc gagggcaagc gcgacctgca gaccggctgt accgtcgcga
     2461 tccagcgtgt atcccgtgac cgcgccctcg ggcggcaatt ggtccgcgat cgcgcccagc
     2521 ggctccaggg ccccgtccag ctccagctcg gccacggcgc gcgggcgagc gcgcagactg
     2581 atgcgtgcgg tcggcgcctt accggggcgc aggtggacga aggggagcac cgcgccgggc
     2641 gcggcaaacg gttcgccgct cagctcctgc cccagcgtct cgatcaggtc gagcgtgttg
     2701 aagtagatgt cctcgtgcag cgcctcggtg gccgagcgcc gctcctcgcg caccccgaag
     2761 ggttcgtcgg tcgcgctgag cgacaggtct acgcgcagct cgtcgaagaa gggctgatcg
     2821 acgacgcgtg gctcgccgcc ggtcgattcc aggatgaagc gcgccacgcg cggcaacacc
     2881 tcgccctggt agtagctcca gaagagatcg agatcggtgg gcaccggctc gatcaattct
     2941 cttcccggct ggcgcgcgat cagcccgccg cccacgacgt ggaccgtgcc ggccgcggga
     3001 tacagcccca ggtagggccg ccggtagctg agcggcgaga acgtctcctc gaggagccgc
     3061 tgcccctcgg tgtcgtaggc gtcgacggcg tagagttcgt ccccgctcca ctcctccagc
     3121 gcgatccgct ccagcggcag ccccagcccc gccgccagca gctcgtcgac cgggaagagc
     3181 tcctgcagcc agcgga
//
LOCUS       DASUBI010000309         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_280433, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000309 DASUBI010000000
VERSION     DASUBI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280433"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VFU72_05945"
     CDS             complement(<1..1952)
                     /locus_tag="VFU72_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5423064.1"
                     /translation="MDNLFGIPMASIMDILLVAFALCLGVGFWIFLRHRVVFKMGVRN
                     VPRRPAQTTLIVIGLMLSTLIIAAALTTGDTLNNSLTGTVYDLTGHVDEVVVQSTGAK
                     ADSSSVGDSFFTQQMVADLDQKLVGNPDIAGTMPILAVPVPTINDRTELHEPSLTLTG
                     IDPAKLDAFGGLKDVNGKAIDLGSLPAGKVVIGKKAADKLDARPGDTLTVFAQNTQHT
                     LTVAAIAPDEIMTGMTDVGGNGGFVMPLPEAQQLSGRAGQISLVAVANKGSVKESVKR
                     SDAAVSALNLALEGTHYKPVAIKQSALKDSELAGNIFTSVFMMLGLFSIAAGVLLIFL
                     IFVLLAAERKPEMGMARAVGLKRRQLTQMFLAEGIAYDLVSALVGAALGVLVAFIIAT
                     AMGKLVGDFFTIKPVATARSLVIAYTLGVVVTFITIAVSSWRVSRLNIVAAIRDIPEP
                     SRQRASRRWLIAGILGVIIGALLIWVGESTKEVFPLSVGVSLLPLSLAVALRRFGVPA
                     RPLYTVASLLVLFFWLAPESWNDAVFGKLHGGIEMFFVSGIMMVAAATMAIIWNAEIL
                     TGRVSLLGRTFSRWLPAVKTAVAYPSASKGRTGMTIAMFSLIVFSLVMMATMNANFTQ
                     IFTTDKAGAGWDISATQAPTNPIQDFP"
     gene            complement(1980..>2322)
                     /locus_tag="VFU72_05950"
     CDS             complement(1980..>2322)
                     /locus_tag="VFU72_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5423065.1"
                     /translation="PKEARARAMQVLDQVGLSEWAGHKPAELSGGQRQRVTIARALVN
                     GPAIVWADEPTGDLDSETADEVLTLMRQLNERNHQTFVLVTHDPRIGALCDRIVRMRD
                     GQIISTNDTDQ"
BASE COUNT          379 a          748 c          817 g          378 t
ORIGIN      
        1 gggaagtcct ggatcggatt ggtcggcgcc tgcgtcgcgc tgatgtccca gccggcgccg
       61 gccttatcgg tggtgaagat ctgggtgaag ttggcgttca tggtcgccat catcaccagc
      121 gagaagacga tcaggctgaa catggcgatc gtcatgccgg tgcggccctt gctggccgag
      181 ggataggcga cggcagtctt gaccgccggc agccagcggc tgaaggtgcg gccgagcagg
      241 ctgacacgtc cggtcaggat ctcggcgttc cagatgatcg ccatggtggc cgcggcgacc
      301 atcatgatgc ccgagacgaa gaacatctcg atgccgccat ggagcttgcc gaagacggcg
      361 tcgttccacg attcaggcgc cagccagaag aagaggacca gcagcgaggc cacggtgtag
      421 agcgggcggg ccggcacgcc gaagcgccgc agcgccaccg ccaggctgag cggcaggagc
      481 gacacgccga cgctcagcgg gaagacttcc ttggtgctct ccccgaccca gatcaggagc
      541 gcgccgatga tcacgcccag gatgccggcg atcagccagc ggcggctggc gcgctggcgc
      601 gacggctcgg ggatgtcgcg gatcgccgcc acgatgttga ggcggctgac ccgccaggag
      661 gagaccgcga tggtgatgaa ggtcacgacc acgcccagcg tgtaggcgat caccaggctg
      721 cgcgcggtgg ccaccggctt gatggtgaag aagtcgccca ccagcttgcc catcgcggtg
      781 gcgatgatga aggccaccag cacccccagc gcggctccga ccagcgccga gaccaggtcg
      841 taggcgatcc cttcggccag gaacatctgg gtcagttggc gccgcttcag cccgaccgcg
      901 cgtgccatgc ccatctccgg cttgcgctcg gcggccagca gcacgaagat caggaagatc
      961 agcagcacgc cggccgcgat cgagaagaga ccgagcatca tgaagacgct ggtgaagatg
     1021 ttgccggcca gctcggagtc cttcagggcg ctctgcttga tggcgaccgg cttatagtgc
     1081 gtgccctcca gcgccaagtt cagcgcgctg acggccgcgt cggagcgctt gacgctctcc
     1141 ttgacgctcc ccttgttggc caccgcgacc aggctgatct ggcccgcgcg cccgctgagc
     1201 tgctgcgcct cgggcagcgg catgacgaag ccgccgttgc cgccgacatc cgtcatgccg
     1261 gtcatgatct cgtccggcgc gatggcggcc acggtcagcg tgtgctgggt gttctgggcg
     1321 aagacggtga gcgtatcgcc cggccgggcg tcgagcttat cggcggcttt cttgccgatg
     1381 acgaccttcc ccgccggcaa cgaacccagg tcaatcgcct tgccattgac gtccttgagg
     1441 ccgccgaagg cgtcgagctt ggcgggatcg atcccggtca gcgtcaacga gggctcgtgc
     1501 agctcggtgc ggtcattgat cgtcggcacc ggcaccgcca ggatgggcat ggtgccggcg
     1561 atatccgggt tgccgaccag cttctggtcg agatcggcga ccatctgctg cgtgaagaag
     1621 gagtcgccca ccgaggatga gtccgccttg gcgccggtcg actgcaccac cacctcgtcg
     1681 acatggccgg tgaggtcgta gaccgtgccg gtcaggctgt tgttgagcgt gtcaccggtc
     1741 gtcagcgcgg cggcgatgat cagcgtgctg agcatcaggc cgatgacgat cagcgtggtc
     1801 tgcgccgggc ggcgcggcac gttgcgcacg cccatcttga aaacgacgcg atggcgcagg
     1861 aagatccaga aacccacgcc gaggcagagg gcgaaggcca ccaggaggat gtccatgatg
     1921 gacgccatgg ggatgccgaa gaggttgtcc actgtgtctg ctcgctccct gcttgccggc
     1981 tactggtcgg tgtcgttggt gctgatgatc tgaccgtcgc gcatgcgcac gatgcggtcg
     2041 cagagcgcgc cgatgcgcgg gtcgtgtgtc accagcacga aggtctggtg gttccgctcg
     2101 ttgagctggc gcatcagcgt cagcacctca tcggccgtct cgctgtcgag gtcgccggtc
     2161 ggctcgtcgg cccagacgat ggcggggccg ttgaccagcg cgcgggcgat ggtgacgcgc
     2221 tgccgctggc cgccggagag ctcggccggc ttgtgcccgg cccactcgct cagcccaacc
     2281 tggtcgagca cctgcatggc gcgggcgcgc gcctccttcg gc
//
LOCUS       DASUBI010000310         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_893978, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000310 DASUBI010000000
VERSION     DASUBI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893978"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1813
                     /locus_tag="VFU72_05955"
     CDS             143..1813
                     /locus_tag="VFU72_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5423066.1"
                     /translation="MERAPLTEDGLPHPFEIGIPMRDGVELAASVYLPGTDTTPTPAI
                     VEITPYDKTSLTAEATLYQRHGYAFVAVDCRGRGKSEGLWRAFFNDPAEGHDIIEWVA
                     AQPWCDGKVGTTGLSYMGWTQWAAASERPPHLVCMVSNSAAGRWQQEIPYTNGCFQLY
                     FGWWVFAVRRRIAERYRSQHVAWDEVLRRLPLDAIGDFIDPSGPTWRDMMDHDRLDAF
                     WQPIRFDGRYDQIDVPCLHVTGWYDLEDLLGAFHHYEHMIEESPARERQWLLVGPWSH
                     VGTRHPSATYGGVEFGRDAAIEMDDIHLRWFDHWLRGNANGVLDDPKVRLFEPGTNVW
                     VESDHWPLGSREESLFLRFDGRDGRLTGTPADGREPGRAYRYDPHDPAPTQLDVLKYP
                     FEDPPLDQSAVEARTDVLVYTSEPLTETLVISGWPHLELFASSDGDDTEWHVKLTDVT
                     PEGRSLKVTQGCLRASYRDSLAEPEPLTPDEVTRFDVELWPTHHAFLPGHRLRVTITS
                     SDFPWFARSLNRFGPLKALGEARVATNTVHHGAPSPSRIRLPVAGDSG"
     gene            complement(1962..2672)
                     /locus_tag="VFU72_05960"
     CDS             complement(1962..2672)
                     /locus_tag="VFU72_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423067.1"
                     /translation="MAQQQLEHYPDTAPSRPGRQPISVVLVDDHVLFRQALRHLLESE
                     PDIRIVGEAGTGDAALGLAEQKHPDVVLMDISMPGMDGVAATRDLKAEDPGVKVIILT
                     MFGEDGHVIRAVRAGADAYLLKNTESTKLVDAIRAAARGASVIEPRMAATLLSEFRRM
                     SSMQDDDGLASLTEREIDLLRLVAQGLSNKEIAYKLKLAESTIKNRLSILFEKLDVKD
                     RTQAAIYALSHGLVNRRE"
     gene            2851..3495
                     /locus_tag="VFU72_05965"
     CDS             2851..3495
                     /locus_tag="VFU72_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423068.1"
                     /translation="MSDFTAGPADDTLVSAARRGDLAAFNLLVDRYERLVYAICLRLL
                     REPALAEDATQETFIRAYGALEQYHGPSLRAWLARIATNRCYDLLRGVRRHPLQSLEA
                     EPGEREPHWSLETPPEDPFQQAVRSELGARLIAALEQLPLEQRVVVLLCDVHGYDYEE
                     IAAATEVAVGTVKSRLSRARARLREILRADQRSRELFEGIGRHYFDDDPGARRR"
     gene            3492..>4224
                     /locus_tag="VFU72_05970"
     CDS             3492..>4224
                     /locus_tag="VFU72_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5423069.1"
                     /translation="MSERETPTGPNGSGRRHETDETLSAYLDGALADQRELARVRAHL
                     AGCAECRRSLSELRALTALLRAQPLPEPPRSFRLTPEQVRRPTPPVPWPIRWQPALRR
                     LTALAAVLLVVVVTSDLIVHGRAQPAASTQAITTSSGASSGGNATAVPALAATAAATA
                     ASAETKSVAAGARPDATPEPFAARAAEASPAAAPAAVAATPAAAAAGASAPVSGQAAS
                     AVTPPAITPAPSETTAPSPAPSAAAA"
BASE COUNT          639 a         1524 c         1427 g          634 t
ORIGIN      
        1 gaaccgccac gcgccccagc ccaattggcg ccagtggctc gatctggtgc ggtgagcgcc
       61 tttgccccct tgcccgagat caccgtggga gagtgacatg gacgcacgcc ggccagcgga
      121 caagacccca tttgagcgag ccatggagcg cgctccgctg actgaagacg gcctgccgca
      181 cccgttcgag atcgggatcc cgatgcggga tggagtcgag ctggcggcga gcgtgtatct
      241 gcccggcact gacacgacgc ccacccccgc catcgtcgag atcacgccct atgacaagac
      301 gagcctcacg gccgaggcga ccctctatca gcgtcatggc tacgccttcg tcgccgtcga
      361 ttgccgcgga cggggcaagt ccgagggtct gtggcgcgcc ttcttcaacg atccggccga
      421 gggccacgac atcatcgagt gggtcgcggc gcagccgtgg tgcgacggta aagtcggcac
      481 gaccggtctc agctacatgg gctggacgca gtgggcagcc gcctcggaac ggccgccgca
      541 cctcgtctgc atggtctcca actcggccgc cgggcgctgg cagcaggaga tcccctacac
      601 caacggctgc ttccagctct acttcggctg gtgggtcttc gccgtgcgcc ggcgcatcgc
      661 cgagcgctac cgcagccagc acgtcgcctg ggatgaggtg ctgcgccgct tgccgctcga
      721 cgccatcggc gactttatcg acccgagcgg tccgacctgg cgcgacatga tggaccacga
      781 ccgcctcgac gccttctggc agccgatccg cttcgacgga cgctatgacc agatcgacgt
      841 gccctgcctg catgtgaccg gctggtacga tctggaagac ctactgggag ccttccacca
      901 ctacgagcac atgatcgaag agtcacccgc gcgcgagcgg cagtggttgc tcgtcggccc
      961 gtggagccac gtgggcacgc gccatccgag cgccacttac ggcggcgtcg agttcggccg
     1021 tgatgccgcc atcgagatgg acgacattca cctgcgctgg ttcgaccact ggctgagagg
     1081 gaacgcgaac ggcgtgctcg acgaccccaa agtgcggctc ttcgagccgg ggacgaatgt
     1141 ctgggtcgag tcggatcact ggccgctcgg ctcacgcgaa gagtcgctgt tcctgcgctt
     1201 cgacggccgc gacgggcggc tcacgggcac gcccgccgac ggccgcgagc cggggcgcgc
     1261 ctatcgctac gacccgcacg atccggcgcc gacgcagctc gatgtgctga agtacccctt
     1321 cgaggatccg ccgctcgatc agtccgccgt cgaggcgcgt accgatgtgc tcgtctatac
     1381 cagcgagcca ctcaccgaga cgctggtcat ctccggctgg ccgcacctcg agctgttcgc
     1441 gtccagcgat ggcgacgaca cggagtggca tgtcaagcta accgacgtga cccccgaggg
     1501 acgctcgctc aaggtgacgc agggctgcct gcgcgcctcg tatcgcgatt cgctcgccga
     1561 gccggaaccg ctcacgccgg acgaagtcac gcgcttcgac gtcgagctgt ggcccaccca
     1621 ccacgccttc ctgcccggcc atcgcctgcg cgtcacgatc acgagcagcg atttcccctg
     1681 gttcgcgcgc agcctgaacc gcttcggccc gctcaaggcg ctcggcgagg cgcgcgtggc
     1741 gacgaacacc gtccatcacg gcgcgccgtc gccctcgcgc atccggctcc ccgtcgccgg
     1801 ggactcaggc tgaggtgcgt gccaaacgga accgaggcga gatcctttcc ctgtcagcct
     1861 gaggccgtag cgaaggatct cttctcgcgt tccctcgcca cggatgttcg ggaacactgc
     1921 tcgccatctg gccggtgcgc ggccgccagc cggccgcgac gttactcgcg tcgattgacc
     1981 agcccgtgcg acaacgcgta gatggcggcc tgagtgcgat ccttgacgtc cagcttctcg
     2041 aagaggatcg agagccggtt cttgatcgtg ctctcggcca gcttgagctt gtaggcgatc
     2101 tccttgttgc tcaggccctg cgccaccagg cgcagcaggt cgatctcgcg ctcggtcagt
     2161 gaggccagcc cgtcgtcgtc ctgcatactc gacatgcggc ggaactcgga gaggagcgtg
     2221 gccgccatgc gcggctcgat gaccgaggcg ccgcgcgcgg cggcgcggat ggcatcgacc
     2281 agcttggtgg actcggtgtt cttgagcagg taggcgtcgg ccccggcacg caccgcccgg
     2341 atgacatgcc cgtcctcgcc gaacatggtg aggatgatca ccttcacccc gggatcctcg
     2401 gccttgagat cgcgggtggc ggccacgcca tccatacccg gcatgctgat gtccatcagc
     2461 acgacatccg ggtgcttttg ctcggcgagc cccagcgcgg cgtcgccggt accggcttca
     2521 ccgacgatgc ggatatctgg ctcgctctcg agcagatggc gcagcgcctg acgaaacaag
     2581 acgtggtcgt cgaccagcac caccgagatc ggctgacggc ccggccgcga cggcgcggtg
     2641 tccggatagt gctcaagctg ctgctgggcc attgccacct ccctcatggt tctcgctggc
     2701 caccgagagg agtgtaggcg ctctgtactg gaccggacat gacccgaaag caccaacagc
     2761 ctgcggtgca aaggtggcca tctcaccacc ggcgacagga ctctgtcata atcgcggaaa
     2821 gacggatcga caccggggtt cgagggggag atgtcggact tcaccgccgg cccggcggac
     2881 gacacgcttg tcagcgcggc acggcgtggc gatctcgcgg cgttcaacct cctggttgat
     2941 cgctacgagc ggctggtcta cgccatctgc ctgcgcctgc tgcgcgagcc ggcgctggcc
     3001 gaggacgcca cccaggaaac gttcatccgg gcctacggcg cgctggagca ataccacggc
     3061 ccctcgctgc gcgcctggct ggcccgcatc gccaccaacc gttgctacga tctgctgcgc
     3121 ggcgtccggc gccacccgct gcagtcgctg gaggccgagc cgggcgagcg tgagccgcac
     3181 tggagcctgg aaaccccgcc cgaggatcct ttccagcagg ccgtgcgcag cgagctgggc
     3241 gcgcgcctga tcgcggccct ggagcagctt ccgctggagc agcgcgtcgt cgtgctcctc
     3301 tgcgacgtgc acggctacga ctatgaggag atcgcggcgg cgaccgaggt ggcggtcggc
     3361 acggtcaagt cgcgcctcag ccgcgcccgt gcccggctgc gcgagattct acgcgccgat
     3421 cagcgctcgc gggaactttt cgagggcatc ggacgtcact attttgacga cgatccggga
     3481 gcgcggcggc gatgagcgag cgagagaccc caactgggcc aaacggcagc ggacggcggc
     3541 acgagaccga cgagacgctc tccgcctacc tcgacggagc gctcgccgac cagcgcgagc
     3601 tggcacgtgt gcgggcccac ctggccggct gcgccgagtg ccgccggtcg ctgtccgagc
     3661 tgcgcgcgct gacggcgctg ctgcgcgcgc agccgctacc cgagccgccg cgctccttcc
     3721 ggctgacgcc cgagcaggtg cgccgtccga cgccgccggt gccctggccg atccgctggc
     3781 aacccgccct gcgccggctg acagcgctgg cggcggtgct gctggtggtg gtggtgacca
     3841 gcgacctgat cgtccacggg cgggcccagc cggcggcatc cacccaggcg atcaccacgt
     3901 cgtccggagc gtcgtccggc gggaatgcga cggccgttcc cgcgctggcc gccaccgcgg
     3961 ccgcgaccgc cgccagcgcg gagaccaaga gcgtggctgc cggggcgcgg ccggacgcga
     4021 cccccgagcc gttcgcggcg cgggcggctg aagcctcgcc ggcggctgcc ccggcggcgg
     4081 tcgcggccac gccggcggca gccgccgctg gcgcgtcggc gccggtcagc ggccaggccg
     4141 cctcggcggt caccccgccg gccatcacgc ccgcgccctc cgagacgact gctccgagcc
     4201 cggcgccgag cgcggccgcg gccg
//
LOCUS       DASUBI010000311         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_257823, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000311 DASUBI010000000
VERSION     DASUBI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257823"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFU72_05975"
     CDS             <1..1034
                     /locus_tag="VFU72_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5423070.1"
                     /translation="DAEVIHLALTVPAAAGVETRLVLGHVGLVLDDLDRLPLRQRSRD
                     WLLWSMERLRKGQALDLEGDLAGLVSDGQLPALFTEMNVALRDVPAEQLERWVLSILG
                     EVGVAVQGGSRAPEEVVAGVIAKLSQHSDEADVRAAFAFLRELAAIHGAPERVLPEIR
                     SLVRRHDLQQQPIDQLERVLELLVAYGHDVDRFELNLGLGRGLHYYTGVLFEVYTADG
                     SSQLCGGGRYDDLAQLLGARQPLPACGFSCGLERLVDEASSLAELSPTDVLVVPASTD
                     AMTTAIRVAEQLRRRGEQVELEVRGRSPNAARRYAMRRGIGRVLLVEADDQPGRQQPL
                     AGAAGEQNV"
     gene            1027..2040
                     /gene="hisG"
                     /locus_tag="VFU72_05980"
     CDS             1027..2040
                     /gene="hisG"
                     /locus_tag="VFU72_05980"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU5423071.1"
                     /translation="MSEPLRLAVASKGSYEDATARFLEQAGLAIWRPNPRQYVGRLSG
                     LSGVEVLFQRVEDIVLKVAENSVDLGITGYDLVAEHGDDDPNLLVVIDDLGFRRCELV
                     VAVPEGWLDIATVADLADLSVALRRSGRALRVATKFPNLTRAFFERHGVHYYSLVDAH
                     GALEAAPVLGYADIITDLSETGVTLRDNRLRVLSGGVVLRAQACLIASRRALRTDAAK
                     LEQARAVVELIEARLRTRAFRLVTANVQGDSPEAVARHVTAQLETAGRQGPTIASVFP
                     KGTEDGVRWWAVTVIVPAERLLAAVDHLRRGGSSGITVQAPEYVFDGCSAAFERLLTA
                     LKE"
     gene            2043..>2471
                     /locus_tag="VFU72_05985"
     CDS             2043..>2471
                     /locus_tag="VFU72_05985"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU5423072.1"
                     /translation="MSLPAGEMVRLFDSEPEARDFLLRRRRLEDTTLPPRVRESVRRI
                     FGADLSASDVVERILADVRREGDRALRRYTLAIDGADLGDLRVSSEEFATARAQVAPD
                     VIEALQLARARIERFHQRQLRTSWMHVDEHGTLGQLLRPIE"
BASE COUNT          352 a          855 c          872 g          392 t
ORIGIN      
        1 ccgacgccga ggtgattcac ctggcgctca cagttccggc ggcggccggc gtcgagaccc
       61 gcctggtgtt ggggcacgtc ggtctggtgc tcgatgacct cgatcgcctg ccgctacgcc
      121 agcgctcgcg cgactggctc ctctggagca tggagcggct gcgcaagggc caggccctcg
      181 atctggaagg cgatctcgcg gggctagtca gtgacggtca actccccgcg ctgttcaccg
      241 agatgaacgt cgcgctccgc gacgtgccgg ccgagcagct ggagcgctgg gtgctgtcga
      301 tcctcggcga ggtcggcgtc gcggtgcagg gcggcagccg ggctccggaa gaggtcgtgg
      361 ccggggtaat cgccaagctg agccagcaca gcgacgaagc ggatgtccgg gcagccttcg
      421 ccttcctgcg cgagctggcg gcgatccacg gagcgccgga gagggtattg ccggagatac
      481 gctccctggt acggcgccac gacttgcagc agcaaccgat cgaccagctc gagcgcgtac
      541 tcgaactgct ggttgcctac ggccacgacg tggatcgctt cgagctgaac ctcggtctcg
      601 gccgtggtct gcactactac accggcgtgc tctttgaagt ctataccgca gacggttcct
      661 cgcagctctg cggcggcgga cgctatgacg acctggcgca actgctcggc gcgcgtcagc
      721 cgctgcctgc ctgcggcttc tcctgcggcc tggagcggct ggtcgacgag gcgtcatcct
      781 tggccgagct gagcccaacc gatgtgctgg tcgtgccggc gagcacggat gccatgacca
      841 ccgccatccg cgtcgccgag caattacgcc gtcggggcga gcaggtcgaa ctggaggtgc
      901 gcgggcgctc gcccaatgcc gctcgacgct acgccatgcg ccgcggcatc ggccgcgtac
      961 tgctggtcga ggccgacgac cagcccggcc ggcagcagcc gttggcgggc gcggcgggag
     1021 agcagaatgt ctgagccgct gcgcctggcg gtcgcctcga aaggctccta cgaggacgcg
     1081 accgcccgct tcctggagca ggccgggctg gcgatctggc ggccgaaccc gcgtcagtac
     1141 gtcggccgct tgagcggttt gagcggagtc gaagtcctgt tccagcgcgt cgaggacatc
     1201 gtgctcaagg tggccgagaa cagtgtcgac ctcggcatca ccggctacga tctggtggcc
     1261 gagcacggcg acgacgaccc caatctgctg gtcgtgatcg acgacctcgg ctttcgccgc
     1321 tgtgagctgg tggtggccgt gccggagggc tggctggaca tcgccaccgt cgccgatctg
     1381 gccgatctct cggtggcgct gcgtcgctcc gggcgcgcct tgcgcgtggc caccaagttt
     1441 cccaacctga cacgcgcctt cttcgagcgt cacggcgtcc actactactc actggtcgac
     1501 gcccacggcg cgctggaagc cgcgccggtg ctcggctacg ccgacatcat cacggacctc
     1561 tccgagaccg gcgtgaccct gcgcgacaac cggctgcgag tgctcagcgg cggcgtggtc
     1621 ctgcgcgcgc aggcctgcct gatcgcctcc cggcgcgcgc tgcgcaccga cgccgccaag
     1681 ctggagcagg cgcgggcggt cgtcgagctg atcgaggcac ggctgcgcac acgcgccttt
     1741 cgtctggtca ccgccaacgt gcagggcgat tcgcccgagg cggtcgcgcg tcatgtcacc
     1801 gcccaactgg aaacggccgg acggcagggg ccgaccatcg ccagtgtttt ccccaagggg
     1861 actgaggacg gcgttcgctg gtgggcggtg acggtgatcg tacccgccga gcggctgctg
     1921 gccgcggtgg atcacctgcg gcgcggcggc agcagcggca tcactgtaca ggcgccggaa
     1981 tacgtcttcg acgggtgctc ggcggccttc gagcgcctgc ttaccgcgct gaaggagtga
     2041 tgatgagcct acccgcgggc gagatggtgc gcctattcga cagcgagccc gaggcgcgcg
     2101 acttcctgct gcggcggcgc cggctcgagg acaccacgct cccgccccgc gtgcgcgaat
     2161 cggtgcggcg catcttcggc gcggacctct ctgcgtctga cgtggtcgag cgcattctgg
     2221 ccgatgtgcg ccgcgagggc gaccgcgcgc tgcgccgcta cacactggcg atcgacggcg
     2281 ccgaccttgg cgacttgcgc gtgtcgtccg aggaattcgc gacggcacgc gcccaggtcg
     2341 cgccggacgt catcgaggcg ctacagctgg cccgcgcgcg catcgagcgg ttccatcagc
     2401 gccaactccg cacctcctgg atgcacgtcg acgagcacgg gacgctcggc cagctgctgc
     2461 gccctatcga g
//
LOCUS       DASUBI010000312         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_914582, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000312 DASUBI010000000
VERSION     DASUBI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914582"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFU72_05990"
     CDS             complement(<1..438)
                     /locus_tag="VFU72_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5423073.1"
                     /translation="MLETIFFGSPEFAVPSLLALAADARYHVGLVVTQPDRGAGRGRR
                     PQPPPVKVAALERGLPVWQPPTLRASEPAERLAALAPDLFVVVAYGEILRRRVLAVPR
                     NGALNVHPSLLPELRGASPIPAAILAGRGETGVSLIQLEARLDA"
     gene            complement(452..955)
                     /locus_tag="VFU72_05995"
     CDS             complement(452..955)
                     /locus_tag="VFU72_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HEU5423074.1"
                     /translation="MKTLKVIVGGEGNVGKTSLIRQYAKGKFSEARNITLGIDITTQQ
                     FSIGGEDVRLAIWDIEGQAGNRPNFYIGAQAAALVYDGTEPASLEALIQWYQRVRRYG
                     SDTPVIVVCNKVDLGLAFPPVWGRVFARCIGARHGFVSAKSGDNVGQLFQLLAESAVD
                     HAALAHR"
     gene            1098..>4929
                     /locus_tag="VFU72_06000"
     CDS             1098..>4929
                     /locus_tag="VFU72_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEU5423075.1"
                     /translation="MSTTRSRRFLFLIAASVVICLALGLRFFGRDWDQGWYMQPDERF
                     MVMVLTDRIHAPDIHQLGQLFDPAHSPLNPRSVDQSGRPQAYAYGSLPLFATDATAAV
                     AGFLTRQDLNSYARVGMVGRSLSALADVITVVLAMILARRLYGRAAAPISGLLLATTV
                     TMIQLAHFFTVDSFVTMFATAVLLAALLLVERPTRLRAIVAGLLIGAALATKISVFTL
                     AIPVALALVLAYRQHGPPRSLVLALRRLLWTGAAALVAFALFEPYAFWQPHAFFNDIS
                     SQWKMANGSADLPYTRQFVGTIPVRYELGNLVHWGLGPLFGIAALVALVVVARRGRKM
                     RPGEIILLAWIVPYFAVISTAEVKYLRYAEPIVPALAILTGGFLAALAVRWPARSWRG
                     AVSLGLTAVVLLGTLGWALAFESIYTRPNSHLAASDWIYRNVPPGSTITAEWWDDSLP
                     LPLANIPPPQAAYKQITLDLYADRPNQQEFDYIAGFLRDSDYIILASDRLAQSIPRLP
                     WRYPVATDYYRLLESGQLGFQLVYESNVMPSLGPLHFNDRGADESFTVYDHPHVRIYK
                     KVTTLSRDDLQQRFASAIAQPWYPARTPPQPTLLLSTPVDQQPAVNDLGWSAAWTSHN
                     LVAVAVWLLVIAALGLLAIPATSVLFGRFADAGWGLSRALGILIAGYAVWIAVSLQLI
                     QFRLPDMLLVLALAIGAEWLILRRQTRQGLRELRARWRMVALSEGLFLAAFAFFLLLR
                     AVNPDLWQTFWGGEKPMEMSFIQALARSAHFPPYDPWYAGGAMNYYYYGFYLMAALWK
                     LTGIPPEIAFQLSIATISAMLVSTVFSLAATAGGDLLRTRRGRWQAGAGVSAVVIEIF
                     VGNLDAARQLLQHSPAAFDFWQSSRVVDYGITEFPYFTSIWADLHPHAINLPFTVLLL
                     ALVYVAFREGLHRWWFAGASLVLGSVMIINSWDMPYGLLVVAAAVLTALVRRDGWTWR
                     AVGLAALAWGLTAGGAYILFQPFFSHFLALVNGVARTAHGTAVAQYGLHFGVMLGVLG
                     AAGVVLVLAQWPRLDPRLRPLDSLSGAILGLASFGVAALTIEQHTLWHFALGALVAAA
                     AGVAFYALLSGGASLPGPRKLWYATATIFTLALGLLLPERTTAALLLLPLAAGLRLWR
                     GRLEDPSTSIAGIFLLGASGITLGADLIYIVDDLNGSPWARMNTIFKFYVEAWTLYAL
                     AAGLALVWLLFSARLGWGQPLGIVAGARGARRPIDITKPQPTMLAARMALVVALCFVG
                     LGFLYPIFGTPARLK"
BASE COUNT          690 a         1765 c         1529 g          945 t
ORIGIN      
        1 ggcatcgagc cgtgcctcca gctggatcag cgagacgccg gtctcgccac gaccggccag
       61 tatcgccgcc gggatcggcg acgctccccg caactccggt aggagcgacg gatgaacatt
      121 gagcgccccg ttgcgcggga ccgccagcac gcgccgccgc aagatctcgc cataggccac
      181 caccacgaag agatcgggag ccagcgccgc cagcctctcc gccggttccg aagcgcgcag
      241 ggtcggcggc tgccagaccg gcagaccccg ctccagcgcc gccaccttca ccggcggcgg
      301 ttgcggacgg cggccgcggc ctgcccctcg gtcgggctgg gtcacgacca gcccgacgtg
      361 atagcgcgcg tcggcggcca gagctaggag cgagggaacc gcgaattcgg gggagccgaa
      421 gaagatcgtt tcgagcaaga gaccgcgagt ctcagcggtg cgccagcgcg gcgtggtcca
      481 cggccgactc cgccagcagt tggaagagtt ggccgacatt gtcgccgctc ttggccgaaa
      541 cgaagccgtg ccgcgcaccg atgcagcggg cgaagacgcg cccccagacc ggtgggaagg
      601 ccaggccgag atcgaccttg ttacacacga caatcaccgg cgtatcgctg ccgtagcggc
      661 gcacgcgttg gtaccactgg atcagcgcct ccaacgaggc cggctcggta ccatcgtaga
      721 ccagcgcggc agcctgcgcg ccgatgtaga agttgggccg gttgcccgct tggccctcga
      781 tgtcccagat cgccagccgc acgtcctcgc cgccgattga gaactgctgc gtggtgatgt
      841 cgattccgag ggtaatattt cgcgcttcgc tgaacttccc cttggcgtac tggcgaatca
      901 gcgacgtttt gcctacgttc ccttcgccgc cgacgatcac cttcagcgtc ttcatcccca
      961 cccgactccc gtcagtgcct gatcgcagtc tcatcggttc atggcaagaa cattagtccg
     1021 agtataggtg gggggcgtat ggggggtcaa cgccgatttg tcccgcttta gtcacgactg
     1081 ctatcatttc gagcgccatg agcacgactc gatcgcgtcg ctttctcttt ctcatcgcgg
     1141 ccagcgtcgt gatctgcctg gcgctcggtc tgcgcttctt cggacgcgac tgggaccagg
     1201 gctggtacat gcagcccgac gaacgcttca tggttatggt cctgaccgac cgcattcacg
     1261 cgcccgacat tcaccagctc ggacagctct tcgatccagc ccactcgccg ctcaacccac
     1321 gcagcgtgga ccagagcggg cggccccagg cctacgccta tggctcgctg cccctctttg
     1381 ccaccgacgc cacggctgcc gtcgccggct tcctgacacg gcaagatctc aatagctacg
     1441 cgcgcgtcgg tatggttggc cgcagcctca gcgcgctggc ggacgtcata accgtcgtcc
     1501 tggccatgat cctggcgcgt cgcctctacg gccgtgcagc tgcgccgatc agcgggctcc
     1561 tgctggccac aacggtcacg atgattcagc tcgcccattt cttcaccgtc gatagcttcg
     1621 tgaccatgtt cgccacggcg gtcttgttgg cggcgctgct tctggtcgaa cgaccgacgc
     1681 ggctccgggc gatcgtggcc ggcctcctga tcggcgccgc gctggcgacc aagatcagcg
     1741 ttttcacgct cgccatcccg gtcgcgctcg cgctcgtgct ggcctaccgg cagcacggcc
     1801 ctccgcgctc actcgtgctg gcgctgcgcc gtcttctctg gactggagcc gccgcgctcg
     1861 tcgcgtttgc gctcttcgag ccgtacgcgt tctggcagcc gcacgccttc ttcaacgaca
     1921 tcagtagcca gtggaagatg gccaacggca gcgcggatct gccctacacc cgtcagttcg
     1981 tgggcacgat tccggtgcgc tacgagctgg gcaatctggt ccattggggt ctcggcccgc
     2041 tcttcggcat cgccgcgctg gtcgcgctcg tcgtcgtcgc ccgtcgtggc cggaagatgc
     2101 gcccgggcga gatcatcctg ctcgcctgga tcgtcccgta cttcgcggtc atctcgaccg
     2161 cggaggtcaa gtatctgcgc tacgcagagc cgatcgtacc cgcgctggcg atcctgaccg
     2221 gcggtttcct ggccgcgctg gcggttcgct ggccggcccg gagctggcgc ggcgccgtct
     2281 cgctcggact aaccgccgtg gtgctgctcg gcacccttgg ttgggcgctc gccttcgagt
     2341 cgatctacac ccgccccaac agccacctgg ccgcatccga ctggatctat cgcaacgtcc
     2401 cgccgggctc gaccatcacc gccgagtggt gggacgactc gctcccgctg ccgctggcca
     2461 acattccccc gccccaggcg gcctacaaac agatcacgct cgatctctac gctgaccggc
     2521 caaaccagca agagttcgac tacatcgccg gttttctgcg cgacagcgac tacatcatcc
     2581 tggcgagtga ccggctggcg cagtcgattc cgcgcctccc ctggcgctat ccagtagcaa
     2641 ccgactacta ccgattgctc gaatcgggtc agctcggctt tcagctggtc tacgaatcga
     2701 acgtgatgcc gtcgcttggg ccgctccact tcaacgaccg cggcgccgac gagagcttca
     2761 ccgtttatga tcacccccat gtgcgcatct acaagaaggt aacgacgctc tcgcgcgacg
     2821 atctccagca gcgcttcgcc tcggccatcg cccagccgtg gtaccccgca cgcacaccgc
     2881 cgcagccgac gctgctgttg agtacgcccg tcgaccagca gcccgccgtc aatgacctcg
     2941 gctggtcagc ggcctggacc agccacaatc tggttgccgt ggctgtctgg ctgctggtca
     3001 tcgcggcgct gggtctgctg gcgattccgg ccacgtcggt cctgttcggc cgctttgccg
     3061 acgccggctg gggactgtcg cgcgcgctcg gtatcctgat cgccgggtat gcggtctgga
     3121 tcgccgtcag cctgcaactg attcagttcc gcctgccgga catgctgctc gtgctggcgt
     3181 tggcgatcgg cgccgagtgg cttatcctgc gccgtcagac acgccagggg ctacgagaat
     3241 tacgcgcgcg ctggcgcatg gtggcgctca gcgaagggct cttcctggct gcttttgcct
     3301 tcttcctgct gctgcgagcg gtcaaccccg atctctggca gactttctgg gggggcgaga
     3361 agccgatgga gatgtccttc attcaggccc tcgcccgcag cgcgcacttc ccgccctatg
     3421 acccctggta cgcgggcggg gccatgaact actactacta cggtttctac ctgatggccg
     3481 cgctctggaa gttgaccggg attccccccg agatcgcctt ccagctctcg atcgccacta
     3541 tcagcgccat gctcgtctcg actgtcttca gcctggcggc caccgccggc ggcgacctgc
     3601 tgcgcacccg tcgcggccgc tggcaggccg gggctggtgt ctcagcggtg gtcatcgaga
     3661 tcttcgtcgg caacctcgac gcggcgcggc aacttctgca acactcaccc gccgcgtttg
     3721 atttctggca gagcagtcgc gtcgtcgact atggcatcac cgaattcccc tacttcacct
     3781 cgatttgggc cgacctccac ccgcacgcga tcaacctccc cttcacggtg ctgctgctgg
     3841 cgctggtcta cgtcgccttc cgtgaagggt tgcaccgctg gtggttcgcc ggagcctcgc
     3901 tggtgctggg ctcggttatg atcatcaact cttgggatat gccgtatggg ctgctggtcg
     3961 tcgcggcggc cgtactcacc gccctggtgc gtcgcgacgg ctggacctgg cgcgccgtcg
     4021 ggctggccgc gcttgcctgg gggctgaccg cgggcggcgc ctatatcctc ttccagccct
     4081 tcttcagcca cttcttggcg ctcgtcaacg gcgtggcgcg caccgcgcac ggcacggccg
     4141 tggcgcaata cggcctccac ttcggcgtca tgctcggggt gcttggtgcc gccggcgtgg
     4201 tgctggtcct ggcgcagtgg ccgcgactcg atcctcgtct ccgaccgctc gacagcctgt
     4261 ctggcgccat cctcgggttg gctagcttcg gcgtggccgc gctcacgatc gagcagcaca
     4321 cgctctggca ctttgccctc ggtgcgctgg tcgcggccgc tgccggagtg gcgttctatg
     4381 cgttgctttc cggaggtgcg tcgctgcccg ggccgagaaa gctctggtat gccaccgcga
     4441 cgatcttcac gctcgcgctc ggcctgctcc tcccggagcg caccaccgcg gcgctcctgc
     4501 tgctgccgct ggcggcggga ctgcgactct ggcggggccg gctcgaagat ccttccacgt
     4561 caatcgcggg tatcttcctg ctcggcgcct ccggcatcac cctgggcgcc gatctgatct
     4621 acatcgtcga cgacctaaac ggctcgccct gggcgcgcat gaacacgatc ttcaagttct
     4681 acgtcgaggc ctggacgctc tacgcgctgg cggccggcct ggcgttggtc tggctcctgt
     4741 tctccgcccg tctcggctgg ggtcagccgc tcggcatcgt ggccggcgcg cgcggcgcgc
     4801 gccggccgat cgacatcacc aagccgcagc caacaatgct ggccgcgcgc atggcgctgg
     4861 tggtcgcgct ctgcttcgtg gggctcggct tcctctaccc catcttcggc actccggcgc
     4921 ggctcaagc
//
LOCUS       DASUBI010000313         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_111242, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000313 DASUBI010000000
VERSION     DASUBI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111242"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFU72_06005"
     CDS             <1..961
                     /locus_tag="VFU72_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423076.1"
                     /translation="MTRNDAYWGPKPAFKNAVFRIIPDIATQIAELLAGRVDIVPAFP
                     LDQIDALQKSGAGTAKVQAILRTAFRGMDVRGRSGSTIFKDKAVRQAVNYACNVKGYT
                     EKLEKGGDLTPGNVNPMAFGYDKTVEPYPYDPKKAKQLLTDAGYKPDSDGIMAKDGQR
                     LEFQFITGPSTVPNNSQLNQAISQDLADVGIKATIQNIGDSTQFTTLVSTNKAVFYQW
                     DWGYFSVFDADGILYDMFHSDSPYAYYSTPELDKLLEEGRGTLDSAKREQVYSQAQHL
                     LHDDAAVLFMFGAHSIWGVSSKVDWTPRSDEIDRLFEAKPKTS"
     gene            1030..1944
                     /locus_tag="VFU72_06010"
     CDS             1030..1944
                     /locus_tag="VFU72_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423077.1"
                     /translation="MRAYIVSRLIQTVVVVFGISLIAFTITYVLGDPLAVLLPLDAPQ
                     ETRIAYRHAMGLDQPLPVQYYHFMSQVATGHFGNSYVVHKPAYDLIMERMPATMLLAV
                     AGLIVALVIAVPLGIIAAYRRGSWVDNLSSLIAVAGSAMPIYWLGLMLIILFGVHLKW
                     LPPSGYGSWKNIILPGFTLGVFLAPITMRLIRSDMLEVLDQDYIRVARAKGVRERAVL
                     TRHALRNAIIPVLSVLGIQFGQLLGGAVVTETTFAWPGVASQVVQAIQNQDRPVVQAG
                     VIILAVAICLVNLLVDIVVVFLDPRIRT"
     gene            1947..2870
                     /locus_tag="VFU72_06015"
     CDS             1947..2870
                     /locus_tag="VFU72_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423078.1"
                     /translation="MNSTSALTRDDAALSELGGGRPSERRKLLRRVWRTKGLVIGGSV
                     VFVIVLIAVFGPFVAPHDPLRGRMLDRLAPPIWAGGTTEYILGTDALGRDELSRLILG
                     ARVSLEAGASGTLVAALFGVALGLLAGYFSGALDWVISSLVNIMLTFPFVLLALAIIA
                     VLGTDFRNLIIVLALTSWPIYTRVIRAQVRALRDREFMTAARVIGVGHARAMLRHALP
                     NTVNAIIVIASLQVAQLIILESFLSFLGLGVQPPTPSWGSMLGESRTYMFDRWWLAAM
                     PGLAIFVTTLAINLLGDGLRDLLDPHGRNTI"
BASE COUNT          531 a         1205 c         1101 g          581 t
ORIGIN      
        1 gatgacgcgc aacgacgcct actggggccc caagccggcc ttcaagaacg ccgtcttccg
       61 catcatcccc gacatcgcca cccagatcgc cgagctgcta gcggggcggg tcgacatcgt
      121 gccggccttc ccgctcgatc agatcgacgc gctgcagaag tcgggcgccg gcacggccaa
      181 ggtgcaggcc atcctgcgca ccgccttccg cggcatggac gtgcgcggcc gctccggcag
      241 caccatcttc aaggacaagg ccgtgcgcca ggcggtcaac tacgcctgca acgtcaaggg
      301 ctacacggag aagctggaga agggcggcga tctgacgccc ggcaacgtca acccgatggc
      361 cttcggctac gacaagacgg tcgagcccta cccctacgat cccaagaagg ccaagcaact
      421 gctgaccgac gccggctaca agcccgactc cgacgggatc atggccaagg acgggcagcg
      481 gctggagttc cagttcatca ccggtccttc gacggtgccc aacaactccc agctcaacca
      541 ggccatcagc caggatctgg cggatgtcgg catcaaggcc accatccaga acatcggcga
      601 ctcgacccag ttcaccaccc tggtcagcac caacaaggcg gtcttctacc agtgggactg
      661 gggctacttc tccgtcttcg acgccgacgg catcctctac gacatgttcc acagcgactc
      721 gccctacgcc tactactcga cgccggagct ggacaagctg ctggaggagg ggcgcggcac
      781 gctcgactcc gccaagcgcg agcaggtcta cagccaggcg cagcacctgc tgcacgacga
      841 cgcggcggtg ctgttcatgt tcggcgccca cagcatctgg ggcgtcagca gcaaggtcga
      901 ctggacgccg cgctcggacg agatcgaccg cctcttcgag gccaagccca agacgtctta
      961 gcgccgcgcg ggtcagcgcg ggcggaggtc ggctctgccc gcgccggcct ggggtggggg
     1021 attgtcgcca tgcgcgccta tatcgtcagc cggctgattc agacggtggt ggtcgtcttc
     1081 ggcatctcgc tgatcgcctt caccatcacc tacgtgctgg gcgatccgct ggccgtgctc
     1141 ctgccgctcg acgcgcccca ggagacgcgc atcgcctacc gccacgcgat ggggctcgat
     1201 cagccgctgc cggtgcagta ctaccacttc atgtcccagg tcgcgaccgg gcacttcggc
     1261 aactcctacg tggtgcacaa gccggcctac gacctgatca tggagcgcat gcccgccacc
     1321 atgctgctgg cggtggccgg tctgatcgtc gcgttggtga tcgccgtgcc gctggggatc
     1381 atcgccgcct accgccgcgg ctcctgggtg gataatctct cctcgctgat cgcggtcgcc
     1441 ggctcggcca tgccgatcta ctggctgggt ctgatgctga tcatcctctt cggggtgcat
     1501 ctcaagtggc tgccgccctc gggctacggg agctggaaga acatcatcct gcccggtttc
     1561 accctgggcg tcttcctggc gccgatcacc atgcggctga tccgctccga tatgctggag
     1621 gtgctcgatc aggactacat ccgcgtagcc cgcgccaagg gcgtgcgcga gcgcgccgtg
     1681 ctcacccgcc acgcgctgcg caacgcgatc atcccggtcc tctcggtgct cggtatccag
     1741 ttcggccaac tgctcggcgg cgcggtcgtg accgagacga ccttcgcctg gccgggcgtg
     1801 gccagccagg tggtgcaggc gatccagaac caggaccggc cggtggtcca ggcgggggtg
     1861 atcatcctgg cggtcgccat ctgcctggtc aatctcctcg tcgatatcgt cgtcgtcttc
     1921 ctcgacccac ggatcaggac gtgagcatga atagcacgag cgcgttgacg cgcgacgacg
     1981 cggcgctcag cgagctgggt ggcgggcggc cgagcgagcg gcgcaagctg ctgcgccgcg
     2041 tctggcgcac gaaagggctg gtgatcggcg gctcggtcgt gttcgtgatc gtcctgatcg
     2101 cggtgttcgg ccccttcgtg gcgccgcacg acccgctgcg cgggcgcatg ctcgaccggc
     2161 tggcgccgcc gatctgggcg ggcggcacga ccgagtacat cctgggcacc gacgcgctcg
     2221 ggcgcgacga attgagccgg ctgatcctcg gcgcgcgcgt ctcgctggag gccggcgcca
     2281 gcggcacgct ggtggccgcg ctcttcggcg tcgccctggg gctgctggcc ggctacttca
     2341 gcggcgcgct cgactgggtg atctccagcc tggtcaacat catgctgacc ttccccttcg
     2401 tgctgctggc gctggcgatc atcgccgtgc tgggcaccga cttccgcaac ctgatcatcg
     2461 tgctggcgtt gacgtcgtgg ccgatctata cgcgtgtcat ccgggcgcag gtgcgggcgc
     2521 tgcgcgaccg cgagttcatg accgcggcgc gggtgatcgg cgtcgggcac gcacgggcca
     2581 tgctgcgcca cgcgctgccc aacacggtca atgcgatcat cgtgatcgcc agcctccagg
     2641 tggcccaact gatcattctg gagtcgttcc tcagcttcct ggggctgggc gtgcaaccgc
     2701 cgacgccctc ctggggcagc atgctgggcg aatcgcgcac ctacatgttc gaccgctggt
     2761 ggctggccgc catgccgggg ctggcgatct tcgtcaccac gctggcgatc aacctgctgg
     2821 gcgacggcct gcgcgacctg ctcgaccccc acggccgcaa caccatctag ctcgcacctc
     2881 cgcctctgag cagcctgatc caggtaatat tgcgcagcca agcgccgtgg cacgattctg
     2941 gctgcgttcc cctcatattt gggggacgaa aggtatggtg ggagatgttt ggaggcatcg
     3001 gcgagttgat cctgatgggc tggctggggc tgatcgtcta tctggtctac aagctgatcg
     3061 agtccatcgg caacgcccgc cacaaagggt gagcgccggg cagccggccg cgccgcgacg
     3121 acacgcacgg cacgcggtat tcctgtaggg gcaggcctct gcgtccgcac ctgccctcgc
     3181 ggcctgccgc cgcacggtgt cgctcgctgc cctggccacc ccgttcgcca cgtggccacg
     3241 cgtccccggt gccggaccgc agaggcaaca gcgcgagggt ggacactggg gtgagcccgt
     3301 acaggggtac acggggccgt gccacatacc ctcgacccgt tcaccagcgt cttcaaccct
     3361 gcccctaccg tgaacccacc cccacgatcc ccgctatttg cgctctcgca aagacccc
//
LOCUS       DASUBI010000314         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_780308, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000314 DASUBI010000000
VERSION     DASUBI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780308"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFU72_06020"
     CDS             complement(<1..392)
                     /locus_tag="VFU72_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5423079.1"
                     /translation="MVEGVAMLAAAETKTIVTRGGVRLHGRVGIDGAKNAALPAMAAA
                     LLTEEECLLDNVPVLEDVLVMIELLRRLGAEVEFDQPNHRLRICAAPVHDHNAPPELV
                     ERMRASFLVSGPLLSRLGRAGSTAPGGC"
     gene            complement(688..1134)
                     /locus_tag="VFU72_06025"
     CDS             complement(688..1134)
                     /locus_tag="VFU72_06025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU5423080.1"
                     /translation="MPLSVEVVTGERLVYEADDVDMVIAPGADGTLGILPRHAPLIST
                     LAAGELRVRRGNQEEELAVFGGFIEVAHNMVRVLADVAELAEEIDVVRAQAARDRAEA
                     RIAQSRTEIDLVRAEAALRRSLVRLRVAQHSRRHGRADVGRVEVGP"
     gene            complement(1310..2728)
                     /gene="atpD"
                     /locus_tag="VFU72_06030"
     CDS             complement(1310..2728)
                     /gene="atpD"
                     /locus_tag="VFU72_06030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5423081.1"
                     /translation="MAQAPVKGATGKIVQIQGVVVDVEFPPDQLPQIYNALNVELEGR
                     GTMVLEVQQHLGNDWVRCVAMSSTDGLRRGVDAVDTGKPIEVPVGEVTLGRIFNVLGE
                     PVDNAGAVEAQIEYPIHRDAPTFEEQSTSIEVFETGMKVIDLIAPFTRGGKTGVFGGA
                     GVGKTVIIMELIRNIAAEHGGYSVFTGVGERTREGTQLWGEMHESGVVDKTVLVFGQM
                     NEPPGARLRVGLTGLTMAEYFRDEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGY
                     QPTLATEMGQLQERITSTKRGSITSVQAVYVPADDYTDPAPATTFAHLDATVSLERSI
                     ADQGLYPAVDPLSSTSRILDPQVVGVEHYTVAREVQRVLQRYRDLQDIIAILGIEELS
                     DEDKLTVTRARKIQRFLTQPMFVAQAFTGQEGRYVDRAETVRGFKEILEGKYDELPEQ
                     AFYMVGKIEEAVQRAEEMAAQG"
     gene            complement(2746..>3575)
                     /gene="atpG"
                     /locus_tag="VFU72_06035"
     CDS             complement(2746..>3575)
                     /gene="atpG"
                     /locus_tag="VFU72_06035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5423082.1"
                     /translation="RNTSQITRAMEMVSASKMRRAQQHVTATRPYAERIAAMIGDVAA
                     AASPQEQAQFPLLVQRPVNRAEIILVTSDKGLAGAFNTNIIRRAVHLMTDEREAGHAE
                     PVLLAVGRRGRDFFNRFRGNVAASYGPIPDQPALLAIRPIVQRAIEDFSSGEVDAVYV
                     VYTRFINTLRQVPEVIRVLPIEVPEGAGDVREYIFEPSAEAVLEALLPRFVEVQIYHA
                     ILESLASEHSARMVAMRNATENARDLVSDLSLSYNRARQAQITREVAEIAAGAAALG"
BASE COUNT          553 a         1267 c         1162 g          593 t
ORIGIN      
        1 ttgcagccac cgggcgcggt cgagccggca cgccccaggc gcgagaggag cgggccgctg
       61 accaggaacg aggcgcgcat acgctcgacc agctccggcg gggcgttgtg gtcgtggacc
      121 ggcgcggcgc agatgcgcaa gcggtgattc ggctggtcga actcgacctc ggctcccagc
      181 cgtcgcagca gctcgatcat gaccagcacg tcttcgagga ccgggacgtt gtcgaggagg
      241 cattcctcct cggtcagcag cgcggccgcc atcgccggca gagcggcgtt cttggccccg
      301 tcgatgccga cgcgcccgtg cagccgcacg ccaccgcgcg tcacgattgt cttcgtctcg
      361 gctgctgcga gcatggccac cccctcgacc accgcccgac ggcgcctcgc cgtcgccgcc
      421 gttagcataa caaacgaagg tgtacgggcg ggtgaggtgg ctggcggtga aggttgagcg
      481 atcggccagg acgaatcctt ctcctgagcg cagagcctac cctgagcttg ccgaagggat
      541 gaccgggaaa cgggatgcag ctcaccggcc aaggcgcgtc gtcgtccggc accgatcctc
      601 gtcgtcagcc agacattaac cctgtcatcc cgaccgcagt ggagggatcc gtccgcacac
      661 ggaagccagc gtcgtcgccg gcccggccta cggtccgacc tcaacgcgcc caacgtcggc
      721 gcggccgtgg cgccgcgagt gctgcgccac gcgcagccgc accagcgagc gccgcagcgc
      781 ggcctccgcc cgtaccaggt cgatctccgt acgcgactgc gcgatgcgcg cctcggcccg
      841 gtcgcgcgcc gcttgggccc gcaccacgtc gatctcctcg gcgagctcgg ccacgtcggc
      901 cagcacgcgc accatgttgt gggcgacttc gatgaagcca ccgaagacgg ccaattcctc
      961 ttcctggttg ccacggcgta cgcgcagctc gcccgccgcc agcgtcgaga tcagcggcgc
     1021 gtggcgcggc aggatgccga gcgtgccatc ggcccccggt gcgatgacca tatcgacgtc
     1081 gtccgcttca tacaccagac gctcgccggt gacgacttca accgaaagcg gcatgtagct
     1141 ctccctgtga caatctgcga cgacctgctt cgctacgttg gcatgcgcga ggagggcaga
     1201 cacggaggtc tgcccctacc gcatggaagc cagcggctgt tcctgtagag gcgcacctgt
     1261 gtgtccgccc tcccacgtgt gcgcctggct ccgtcgtagg ccctcgcgcc tacccctgcg
     1321 ccgccatctc ctcggcccgc tgcaccgctt cctcgatctt cccgaccatg tagaaggcct
     1381 gctcgggtag ctcgtcgtac ttgccttcca gaatctcctt gaagccgcgc acggtctcgg
     1441 cccggtcgac gtagcggccc tcctggccgg tgaaggcctg agccacgaac atcggctggg
     1501 tcaggaagcg ctggatcttg cgcgcgcgcg tgaccgtgag cttatcctcg tcactcagct
     1561 cctcgatgcc gaggatggcg atgatgtcct gcaggtcgcg gtagcgctgc aagacgcgct
     1621 gcacctcgcg ggcgacagtg tagtgctcga cacccaccac ctgcgggtca aggatgcgcg
     1681 aggtcgagga gagtgggtcc accgccgggt agagcccctg gtcggcgatc gagcgctcca
     1741 gcgacacggt cgcgtcgagg tgggcgaagg tcgtcgccgg cgccgggtcg gtgtaatcgt
     1801 cggccggcac gtagaccgcc tgcaccgacg tgatcgagcc gcgcttggtc gaagtgatgc
     1861 gctcctgcag ctgccccatc tcggtggcca aggtcggctg gtaccccacc gcgctcggca
     1921 tgcggcccag gagcgccgag acctctgaac cggcctgcac gaagcggaag atgttgtcga
     1981 tgaagaggag cacgtcgcgc ccctcgtcgc ggaagtactc tgccatcgtc aggccggtca
     2041 gcccgacgcg caggcgcgcc cccggcggct cgttcatctg gccgaacacg agcaccgtct
     2101 tgtcgaccac acccgactcg tgcatctcgc cccagagctg cgtgccctcg cgggtgcgct
     2161 cgcccacccc cgtgaagacc gagtagccgc cgtgctcggc ggcgatgttg cggataagct
     2221 ccatgatgat gacggtctta ccgaccccgg cgccgccgaa gacacccgtc ttgccgcccc
     2281 gggtgaaggg ggcgatcaga tcgatcacct tcataccggt ctcgaagacc tcgatgctgg
     2341 tggactgctc ttcgaacgtc ggcgcgtcgc ggtggattgg gtactcaatc tgcgcctcga
     2401 ccgcgccggc gttatcgacc ggctcgccga gcacgttgaa gatgcggccc agtgtgacct
     2461 cgccgaccgg cacctcaatc ggcttgccgg tatcgaccgc gtcgaccccg cggcgcaggc
     2521 catcggtgct cgacatcgcc acacagcgca cccagtcgtt accgaggtgc tgctgcacct
     2581 ccagcaccat cgtgccgcgg ccttccagtt ccacgttcaa tgcgttgtag atctgcggga
     2641 gctgatcggg tgggaactcg acatcgacca ccacgccctg aatctggacg atcttacccg
     2701 tggctccttt gaccggtgct tgtgccatgt tgatgctcct tctgcctaac cgagcgccgc
     2761 ggcaccggcc gcgatttcgg ccacttctct ggtgatctgc gcctgacggg cgcggttgta
     2821 gctcaaggac aggtcgctca ccaggtcgcg cgcgttttcg gtcgcgttcc gcatggcgac
     2881 catgcgcgcg ctgtgctcgc tagccagcga ctccaggatc gcgtgataga tctgcacctc
     2941 gacaaagcgc ggcagcagcg cctccagcac cgcctccgcg ctcggctcga agatgtattc
     3001 gcgtacgtca ccggcgccct ccggcacctc gatcggcagc acccggatca cctccggtac
     3061 ctgacggagc gtgttgatga aacgcgtgta gactacgtag accgcgtcga cctctccgga
     3121 gctaaagtct tcgatggccc gctgcacgat cggccggatc gccagcagcg ccggttgatc
     3181 ggggatcggg ccgtaggacg ctgccacgtt gccgcgaaag cgattgaaga agtcgcgccc
     3241 ccgccggccg acggccaaca gcaccggctc ggcgtgcccg gcctcgcgct cgtcggtcat
     3301 caggtgcacc gcgcggcgga tgatgttggt gttgaaggcc ccggccagcc ccttgtccga
     3361 cgtgaccagg atgatctcgg cgcggttgac cgggcgctgc accagcagcg ggaactgcgc
     3421 ctgctcctgg ggcgaggccg ccgccgccac atcgccgatc atggcggcga tgcgctcggc
     3481 gtagggccgg gtcgcggtca cgtgctgctg ggcgcggcgc atcttggagg ccgacaccat
     3541 ctccatcgcg cgcgtgatct gcgacgtatt gcgaa
//
LOCUS       DASUBI010000315         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1106053, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000315 DASUBI010000000
VERSION     DASUBI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106053"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFU72_06040"
     CDS             <1..1140
                     /locus_tag="VFU72_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5423083.1"
                     /translation="RDKDMQPPLYAKLDIALARGEGVWLWDIDGNRYLDMMSNYGVNI
                     LGHSHPAVVNAIARQASVLMNCHQSFANQTRVALLEKLTAIAPAGLNKAFLSNSGTEA
                     VEAALKFAYVATGRGKLVAAKRGYHGRTLGALAATAEKKYREPFLQELSEAEHVAYGN
                     LDELAQAVDERTAAVVLEPVQGEGGIYPAPAEYLQAARELVTERGALLIFDEVQTGFR
                     TGSWFACQASGVVPDLLATSKGLGNGVPIGATLMTERIAATLEGGVHGTTFGGNPLAC
                     AAALATLEEIERAHLLEQSAEVGRYLVEQIKALQHPKIRDVRGRGMMIGVELKARATP
                     VLRGLQERGVLALMAGSLVIRFLPPLILDQAQADLAVQTLADALG"
     gene            complement(1259..1780)
                     /locus_tag="VFU72_06045"
     CDS             complement(1259..1780)
                     /locus_tag="VFU72_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423084.1"
                     /translation="MGFAYHPEAWHDFYIALAGACAALWGLIFVAISLHPGRVADNPI
                     HRNRARLNLIGLYFLLVVALLALIPGQPAAALGIELALIWILWTVVVGMANLRLLRRL
                     ERVPGGIRARMAVVLVMSACGICSGVTLWLGAGGGLLWNVAANVLGLAMALTTAWSVS
                     FSEDPRESGGSKG"
BASE COUNT          360 a          746 c          745 g          295 t
ORIGIN      
        1 cgcgacaagg acatgcagcc gccgctctac gccaagctcg acatcgcgct ggcgcgcggc
       61 gagggcgtct ggctctggga catcgacggc aaccgctacc tcgacatgat gagcaactac
      121 ggcgtcaaca tcctcggcca cagccacccg gcggtggtca acgccatcgc ccgccaggcg
      181 tcggtgctga tgaactgcca ccagtcgttc gccaaccaga cgcgcgtggc gctcctggag
      241 aagctgaccg cgatcgcgcc cgccgggctg aacaaagcct tcctctccaa ctcgggcacc
      301 gaggcggtcg aggcggcact caagttcgcc tacgtcgcca ccgggcgcgg caagctggtg
      361 gcggccaagc gcggctacca cgggcgcacg cttggggcgc tggcagccac cgccgagaag
      421 aagtatcgcg agcccttcct gcaggagctc tccgaggccg aacacgtcgc ctacggcaac
      481 ctcgatgagc tggcccaggc ggtcgacgag cgcacggccg cggtggtgct ggagcccgtg
      541 cagggtgagg gtggcatcta cccggctccg gccgagtacc tgcaggcggc ccgcgagctg
      601 gtcaccgagc gcggcgcgct gctgatcttc gacgaggtgc agaccggctt ccgcaccggc
      661 tcctggttcg cctgccaggc ctcgggcgtg gtgcccgatc tgctggcgac ctccaagggg
      721 ctgggcaacg gcgtgccgat cggcgccacg ctgatgaccg agcgcatcgc cgcgacactg
      781 gaggggggcg tgcacggcac caccttcggc ggcaacccgc tggcctgcgc cgccgccctg
      841 gcgacgttgg aggagatcga gcgggcacat ctcctggagc agagcgccga ggtgggccgc
      901 tacctggtcg agcagatcaa agcgctccag caccccaaga tccgcgacgt gcgcgggcgg
      961 gggatgatga tcggcgtcga gctgaaggcg cgggccacgc cggtgctccg gggcctgcag
     1021 gagcgcgggg tgctggcgct gatggccggc agcctggtga tccgcttcct gccgccgctg
     1081 atcctcgatc aggcgcaggc cgacctggcc gtccagaccc tggccgacgc gctcggctga
     1141 tccgtccggc ggaccggtgc gcctgctccc tctctcgatc cggctgggga gcggaggcca
     1201 aatcgcgcaa ctccggcgcc cggccccctg ctgcgagcgg caggcttcga gcgccggttc
     1261 agcccttcga gccgccactc tcccggggat cctcggagaa cgacaccgac caggcggtgg
     1321 taagcgccat cgccagcccc agcacgttgg ccgccacgtt ccagagcagg ccgccgcccg
     1381 cgcccagcca cagtgtcacc ccggagcaga tgccgcaggc cgacatcacc agcaccacgg
     1441 ccatgcgcgc gcggattccc ccgggcacgc gctcgagtcg acgcaacagg cgcaggttcg
     1501 ccatgccgac gacgaccgtc cagaggatcc agatcagcgc gagctcgatc ccgagcgccg
     1561 cggccggctg gccgggtatc agggccaaca gcgcgaccac cagcaggaag tagaggccga
     1621 tcaggttcag ccgggcgcgg ttgcggtgga tagggttgtc ggcgacccgt ccgggatgca
     1681 gcgagatagc gacgaagatc agcccccaga gcgcggcgca ggcgccggcc agcgcgatgt
     1741 agaagtcgtg ccaggcctcc ggatgatagg caaatcccat gtccgcgtgt ccccgaatca
     1801 tcgtgcaccg acgcgccgcg acggcaaacg ataccgcaga acgtcgggag gagcgcgccg
     1861 gcgccgagct cgcggcgatc gagcggcatg ggagccgggt gaggacggat ccttcgacaa
     1921 gctcaggatg acagggtttg attggggcct accggacgag gaagatcatg gtccatgact
     1981 catcctcctc gttaacctct cactaaccct gtcatcctga gtggttcgac cgtcatgatt
     2041 tgtccggttc gaggaccgga tagagccgct tgagtttgat gcgggcatcg gcgggggtga
     2101 actgccaatc gatggtgacc gccgcggcgt tgcgggcctg caccca
//
LOCUS       DASUBI010000316         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_997004, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000316 DASUBI010000000
VERSION     DASUBI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997004"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFU72_06050"
     CDS             complement(<1..869)
                     /locus_tag="VFU72_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5423085.1"
                     /translation="MAAPKPFTPITVAEAVHRYRDALHLRVATGALSPATRDAYVRDL
                     AEAAALLGLDTVLDDVEAEDVEYALVRIANAPDRRFTRGRKAGPGGEPAPGRGLQARA
                     RWTAAVRGLFAWAAEQGYVRSDPMPGVSRVRVPTRAVGARLGLTVEQAQALQETPQAA
                     ASASALRADQRLTFRDEVILRLLTETGPRVAELCAANLDDVRQHELTGTPVLRIRNGK
                     GGRSRDVPLSAALVQLLGEYQQHERAAPPAADAEPDRADAARALVVTIRGRRMTPRDV
                     QRMVERRVKQMPA"
     gene            complement(1451..2848)
                     /locus_tag="VFU72_06055"
     CDS             complement(1451..2848)
                     /locus_tag="VFU72_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEU5423086.1"
                     /translation="MSTAPFHLTGNHAPVDAERTAHNLPVTGAVPPELTGWYIRNGPN
                     PRTPSGHWFTGEGMLHAVRLQGGRATCYRNRWVRTASLQDPTRRPYRPDGTQDLAAGP
                     ANTHLIRHAGRTFALVESALPYEISCNHQDLTTIGPHDFAGRLTTSMTAHPKTCPDSG
                     ELHFFGYGALTPPHVTYHRADATGNLIISRPLDAADIPTHTMMHDMALTTHYVVFLHL
                     PVVFDKTKAAAGGMPYHFAPDHPARIGLLRRADPYGPVHWFEVEACYVFHVVNAYEDA
                     RGRVRLHVVRYPHLWWEGHGKADASLWCWTLDPEGYGGGGSGTSVREEQLDDRPCEFP
                     RIDDRLTGHAARFGYVTDVGEAADGGGRGTDARRAGAILRYDLTTGSATAHEFAAGRT
                     PGEACFVPRAPGLTSDDGWLLSYVYDATTDRSDLVILDTADLTGAPAAVVHLPWRVPF
                     GFHGNWLPDGVAGEW"
     gene            complement(2845..>3342)
                     /locus_tag="VFU72_06060"
     CDS             complement(2845..>3342)
                     /locus_tag="VFU72_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423087.1"
                     /translation="PGWAACAALAVALWLVAAQRRAGRAWDALVIECSAIVFLAAYAI
                     TGFASPHSDFLAHYGGPLSSLWLAATAWGSLAIGHPFTLGIARTQTPPSVWRTALFRR
                     VNRVITTVWAAAFTACGIGTALLRHYQPDADTARTALIAASFVLPVLFTLRFPQAARA
                     RWAAE"
BASE COUNT          475 a         1045 c         1312 g          510 t
ORIGIN      
        1 tccgcgggca tctgcttgac gcggcgctcg accatgcgct gcacgtcgcg cggggtcatg
       61 cggcggccgc ggatcgtcac caccagggcg cgcgccgcgt cggcgcggtc gggttcagcg
      121 tcggcggccg ggggagcggc acgctcatgc tgctggtatt cgccgagcag ctggaccagg
      181 gccgcggaca ggggaacgtc ccggctgcgg ccgccttttc cgttgcggat gcgcaggacc
      241 ggggtgccgg tgagctcgtg ctggcggacg tcgtccaggt tcgccgcgca caactccgcc
      301 acacgcgggc cggtctcagt cagaagcctg aggatcacct catcgcggaa cgtcaggcgc
      361 tgatcggccc gcagcgcact ggcggaagcc gccgcctgag gtgtctcctg caacgcctgc
      421 gcctgctcca cggtcaaacc caggcgcgcg ccgaccgccc gcgtgggaac ccgcacccgc
      481 gagacgccgg gcatcggatc ggaccgtaca taaccctgct cggcagccca cgcgaacagg
      541 ccccgcacag cggcggtcca gcgcgcccga gcctgcaacc cgcgcccggg agccggctca
      601 ccgccgggcc cggccttgcg cccgcgcgtg aaacgccggt ccggcgcgtt ggcgatgcgt
      661 acgagcgcgt actcgacgtc ttcggcctcg acgtcgtcca ggacggtgtc cagccccagc
      721 agcgcggcgg cctcggcgag gtcgcgcacg tacgcgtcac gcgtcgcggg cgagagcgca
      781 ccggtggcca cgcgcaggtg gagcgcgtcg cggtagcggt ggacggcttc ggcgacggtg
      841 atgggggtga agggtttggg ggcggccact ggtctatcga agcagatggg gaggacaggt
      901 cggagccagg taacttgaca taatgtacat tatcgggcaa tcggatcgag gggtggagcg
      961 ggggttcggg atgtcggttt gtatcgccga tggcagcacg aggttcacgg agcgctgcgc
     1021 gattgcgcac atgcggcgga ttgctgggcc gctgcactgt gcagcgttca tgatcaaggc
     1081 tggcgatggt gtggagatcg ggaacagacc agcgggccgt ggcagatcgg tggtgaagat
     1141 ggcggcgggt gcggcagtcg gcgtttggga ggtggattca ggctgaaggc gtgtggtgag
     1201 tggcgctgga gccgcggtcc tggcttcggc gcgcgccggc tgtggtcgct ggccttctca
     1261 gaaccctgtc gtgtaatgcg ggttatacgg cttgagatgt gtgggcagta aagagcgctg
     1321 agcggcagcc ggcaacagcg cgccgaacct agccgccgaa gccctgcgcc cagcccacca
     1381 agcagtttgt cagacccctg gctgcttcga taccgcagat cagaaataga tcggtgcggc
     1441 ggcaggacgg tcaccactcc cccgcgacgc cgtccgggag ccagtttcca tggaacccga
     1501 acggcacccg ccacggcaga tgcaccaccg ccgccggtgc tccggtcagg tcggcggtgt
     1561 ccaggatcac caggtcgctg cggtcggtgg tggcgtcgta gacgtagctg aggagccagc
     1621 cgtcgtcgct ggtcaggcct ggtgctcggg ggacgaagca ggcctcgccg ggggtgcgtc
     1681 ctgctgcgaa ttcgtgggcg gtggccgagc ctgttgtcag gtcgtagcgc aggatcgctc
     1741 ctgcgcggcg cgcgtcagtg ccgcggccgc cgccatccgc agcctcgccg acgtccgtga
     1801 cgtacccgaa gcgtgccgcg tggccggtca ggcggtcgtc gatgcggggg aactcgcatg
     1861 ggcggtcgtc cagttgctcc tcgcggacgc ttgtacctga gccgccgccg ccatagccct
     1921 caggatcgag cgtccagcac cacaacgacg catcggcctt cccatgcccc tcccaccaca
     1981 agtgcggata ccgcaccaca tgaagccgca ccctcccccg cgcgtcctcg taggcgttca
     2041 ccacgtggaa cacgtagcag gcctccacct cgaaccagtg caccggcccg tacgggtccg
     2101 cgcggcgtag cagcccgatg cgggccgggt ggtctggggc gaagtggtat ggcatgccgc
     2161 ctgcggctgc tttggtcttg tcgaagacga ctggcaggtg caggaagacc acgtagtggg
     2221 tggtcagggc catgtcgtgc atcatcgtgt gggtggggat gtcggcggcg tccagggggc
     2281 ggctgatgat gaggtttccg gtggcgtctg ctcggtggta ggtgacgtgg ggcggggtga
     2341 gggctccgta gccgaagaag tggagttcgc cgctgtcggg gcaggttttg gggtgggccg
     2401 tcatggaggt tgtcaggcgg cctgcgaagt cgtgggggcc gatggtggtc aggtcctggt
     2461 ggttgcagga gatctcgtag ggcagggcgg attccaccag ggcgaaggtg cggccggcgt
     2521 ggcggatcag gtgcgtgttg gccgggcctg ccgccaggtc ttgggtgccg tcggggcggt
     2581 aggggcggcg ggtcgggtct tgcagggacg ccgttcgcac ccagcggttg cggtagcacg
     2641 tggccctgcc gccctgcagc ctcaccgcgt gcagcatccc ctcccccgtg aaccagtgcc
     2701 ctgacggggt gcgcgggttg gggccgttgc ggatgtacca gccggtcagc tccggcggga
     2761 cggcgccggt caccggcagg ttgtgcgcgg tgcgctcggc gtcgaccggg gcgtggttgc
     2821 cggtcaggtg gaagggtgcg gtgctcattc ggcggcccag cgggcgcgcg ccgcctgcgg
     2881 gaagcggagg gtgaacagga ccggcaggac gaatgaggcg gcgatcaggg cggtgcgggc
     2941 ggtgtcggcg tcaggctggt agtggcgcag cagggcggtg ccgatgccgc aggcggtgaa
     3001 cgccgcggcc cagactgtgg tgatgacgcg gttgacgcgc cggaacagtg cggtgcgcca
     3061 taccgacggc ggggtctggg ttctggcgat gcccagggtg aaggggtggc cgatggccag
     3121 ggagccccag gctgtggcgg ccagccacag cgaggacagc ggcccgccgt agtgggcgag
     3181 gaagtccgag tgggggctgg cgaatccggt gatggcgtag gcggccagga acacgatggc
     3241 gctgcactcg atcaccagcg cgtcccaggc ccggccggcc cggcgctggg ccgcgaccag
     3301 ccacagcgcc accgccagcg ccgcgcaggc ggcccagccg gg
//
LOCUS       DASUBI010000317         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_779563, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000317 DASUBI010000000
VERSION     DASUBI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779563"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VFU72_06065"
     CDS             <1..1402
                     /locus_tag="VFU72_06065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEU5423088.1"
                     /translation="ARRSFAPSWEASAAELQAAAMQPPGWLERVERDHDNVRAALIWL
                     LDNRRGEDALRLGAAFWQFWSLRGYLAEGRMWLQRILDLPGGDVSLRIRVLLGASLLA
                     FRQDDLASASADDAAALRLARETGDRAAIAVALYRLGDDARTRGELATARTLLEAALE
                     ASRLAGNPSVEAWSRFGCGNVALAAGEYAAACTWLAEALASFRRLGIQASAATTTRRL
                     GVVHYLLGDHAAGRSLLEEGLAGVRRAGDQGQVVMALWELGAIASNEGDFTHARALLT
                     DGLQLARELGDQARVCWYLEGFARLAAAQGQTHRALRLASAATALGKEYPRHASGWHA
                     PAMALPQIAGDYAVLEHQLRTARTRLGTPAADAWATGRTLSLDEATAAALLPEPAIAP
                     QPAHGSDGQIPLGLTARELEVVQLVADGKTNRQIAEALFVSDKTVKRHLDNIFAKLGV
                     SSRAAAAAAVLRAQRA"
     gene            1583..>2439
                     /locus_tag="VFU72_06070"
     CDS             1583..>2439
                     /locus_tag="VFU72_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423089.1"
                     /translation="MKRVTALQLVTCVLVVLLLVACGSSAPLPASPTTAASSTSATAS
                     PAAAPPPVPTVQAVEPATGAGRTPVKIAVSGTIAEAPIYVATERGYFAENGISPVFVS
                     FDSAARAVPALSTGQIDVASGSLSSGLFNAVDRGVGIKLVAAQSVNSTCAHSSSWLLV
                     RKDLADSGAVRTAADLRGRKIAVSSKAGTLEYVLDTLLRQGGLQPTDVEIVEMGFGDM
                     GAAFGGHAIDAAFGAEPTATSYVDKGLATKWLCGADIIPDMQITYIQYSSQFANQHTD
                     LAQRWMAAYR"
BASE COUNT          373 a          848 c          825 g          393 t
ORIGIN      
        1 ggcacgccgc agcttcgcgc cgagctggga ggcatccgcg gctgagttgc aggccgcggc
       61 aatgcagccg cccggctggc tcgagcgtgt ggaacgcgac cacgacaacg tgcgggccgc
      121 gttgatctgg ctgctggaca atcgcagggg tgaggacgcg ctgcggctgg gcgccgcgtt
      181 ctggcagttc tggtccctgc gcggatacct ggctgaaggg cggatgtggt tgcagcgcat
      241 cctcgacctc cccggtggcg acgtgtcgtt acgcatccgt gtcctgctcg gcgcgagcct
      301 gctggcgttt cgccaggacg acctcgcgtc ggcgagcgcg gacgatgcgg cagcactccg
      361 gctcgcgcgc gaaaccggcg atcgtgcggc gattgcggtc gcgctctacc gcctcggcga
      421 cgacgctcgt accaggggcg aactcgccac cgcacgcacc ctgctcgagg cagcgttaga
      481 ggccagccga ctggccggca atcccagcgt cgaagcctgg agccggtttg gctgcggcaa
      541 cgtagcgctt gctgcgggcg aatacgccgc tgcttgcacc tggctggcgg aggccctggc
      601 gagctttcgc cggttgggca tccaggcgag cgcggccaca accacgcgac gacttggcgt
      661 tgtgcactac ctgctgggcg atcacgccgc cggccgttca ctgctcgagg agggactggc
      721 cggagtgcgc cgggccggcg accaggggca ggtcgtgatg gcgctgtggg aactgggagc
      781 catcgccagc aacgagggcg acttcaccca cgcccgggcc cttctgaccg acggactcca
      841 gctcgcgcgc gagctcggtg accaggcacg cgtgtgctgg tacctggagg gctttgcccg
      901 gctcgcggcg gcgcaggggc aaacccaccg ggcactccgc ctcgcgagcg cagccactgc
      961 gctgggcaag gagtacccgc gccatgcgtc cggctggcac gcgccggcca tggcgctccc
     1021 ccagatcgct ggcgactacg ccgtgctgga gcaccagttg cggacggcga gaactcggct
     1081 cggcacaccg gcggcagacg cctgggcgac ggggcgtacg ctgtcacttg acgaagcaac
     1141 cgccgccgcg ctgctgcccg agccagccat cgcaccacag cctgcccatg gtagcgacgg
     1201 gcaaattccg ctcggcctta ccgcgcgtga gctggaagtg gtgcagctcg tcgcggacgg
     1261 taaaacgaat cgccagatcg ccgaggcgct gttcgtgagc gacaagacgg tcaagcgcca
     1321 tctggacaat atcttcgcca aactcggggt ctcctcccgc gcggccgccg ccgccgctgt
     1381 gctgcgcgct caacgcgcct gatagccttg catgccggcc ggtatggacc attcggccca
     1441 tggcgcggag gtgtacgcga atgggccgat ccgccgatgc cctgtcctcc agcgagcgat
     1501 acgctctcgg cattgccatt gtccggggca ggcgtccaga aacgtcttcg cgacgcttcc
     1561 ccacccgccg aaggaggctg tcatgaagcg tgttaccgcc ctccagcttg tgacatgtgt
     1621 cctcgtcgtc ctgctcttgg tcgcgtgcgg ttcgagcgcg ccgctgcccg cgtcaccgac
     1681 cacagccgcg tcaagcacaa gcgcaacggc ctccccggct gctgctccgc ccccggtgcc
     1741 cacggtccag gccgtcgaac ccgccactgg cgccggccgg acgccggtca agatcgcggt
     1801 ttccggcacg attgccgaag cgccgatcta cgtcgccacg gagcgcggct acttcgccga
     1861 gaacggcatc tcgccagtat tcgtctcgtt cgattcagcc gctcgcgccg tcccagccct
     1921 gtccaccggc cagattgacg ttgcgtccgg ttcgctctcg tccgggttgt tcaacgccgt
     1981 cgatcgtggt gtcgggatca aactggtggc ggcgcaatcg gtcaatagca cctgcgcaca
     2041 ttcgagctcc tggctgctgg tacgcaagga tctggcggac agcggggcgg tccggactgc
     2101 ggcggacctg cgcggccgga agatcgccgt atcctcgaag gccggcaccc tcgagtatgt
     2161 cctcgatacc ttgctccgcc agggcggctt gcagcccacg gacgtcgaaa tcgtcgagat
     2221 gggcttcggc gacatgggcg cggcgtttgg cggccacgcg attgatgccg ccttcggggc
     2281 cgagccgacc gcgaccagct acgtggacaa agggctggcg acgaaatggc tatgcggcgc
     2341 cgacatcatc ccggatatgc agatcaccta tatccagtac tcgtcccagt ttgccaacca
     2401 gcacaccgat ctggcccaac gctggatggc ggcgtaccg
//
LOCUS       DASUBI010000318         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_638829, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000318 DASUBI010000000
VERSION     DASUBI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638829"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1005
                     /locus_tag="VFU72_06075"
                     /pseudo
     CDS             49..1005
                     /locus_tag="VFU72_06075"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
     gene            1000..1470
                     /locus_tag="VFU72_06080"
     CDS             1000..1470
                     /locus_tag="VFU72_06080"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEU5423090.1"
                     /translation="MRGEWEEWAAPQPAQSVQSARARQPARGKRPPRPPRPRPYRSER
                     GDLIYVGHNGRQNEIVTFELAGPDDTWLHARGMPGAHVVVRWAGAESPELLEQAASLA
                     AWYSAGRAATSVEVDAAPRRHVRKLKGAGPGMVTYRHERTINARPRAPEELGLA"
     gene            complement(1572..>2267)
                     /gene="tatC"
                     /locus_tag="VFU72_06085"
     CDS             complement(1572..>2267)
                     /gene="tatC"
                     /locus_tag="VFU72_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5423091.1"
                     /translation="EQFAEMTLQEHLEELRSRLVRSALAVMVGSVVGLLLAWRIIHLM
                     QRLSNVKEFYAITPTESFTSWMKVGLYFGIALAMPVLIYQFVSFLAPGLTRKEKRVLY
                     RFLPLIVLMFALGVAFAFFVVVPRALDWLAHFGNGTITSQLRPEEVISFYTTLILWCG
                     LVFETPVVMYVLAKLHIVTHQFLAHIRRYMLIVVMVAAAIITPTPDPFNMFMVAIPMY
                     ALYEFGILLAKWA"
BASE COUNT          386 a          797 c          808 g          276 t
ORIGIN      
        1 agcggccacg ggcgtgacga ggacggcgac gacgacggca ccgagggggt gatcgtctac
       61 accgatctct acgttgagct gatgggccgg cgcagcaaca tcatcctgac cgacgagacc
      121 ggtcgcatcc tcgacgcggt caagcgcgtc accgcggaga tgagccgggt acggccggtt
      181 ctgccgggcc ggcagtacgc gccgcccccg ccacaggcca agcgcgaccc gcgcggactg
      241 acgccggccg agctgggcgc gctgctctcc gccgccccgc ccggcgagcg cgcggccgat
      301 ctactcgtgc gcgagctggc cggcatcagc ccacaggtgg cgcgcgaggc ggtctaccgc
      361 gccgttggcc gggaggatgg cgccatctcg gaaatggacg ccgcacggct ggccggcgcg
      421 ctggtggacc tgctggcgcc gctctccagc ggcacctggc agccctcgct ctacctggag
      481 gacggccagc cggtcgcctt cgctccgctg cgcctggcga gcctggccag ccgctacgac
      541 gaggaagcca cgccgacgct ctcggccgcc agcgagcgcg ccagcaacgc cggggccgag
      601 ccggcccacc tgcgctacgc ccgccggcgc gagaagctgg tggcgcagat cggcgccgcc
      661 gccgaccgcg tgcgcgcccg gctgcactcg ctgcgcgagg aggaggagcg ggccagcgcg
      721 gccgagcgct ggcgcgagca gggcgagctg atctacgcct acgtctagca gctgtcgccg
      781 gggcagacag aactggtggt cgaggaccgg cgcatcccgc tcgatccctc catgacgccg
      841 agcgagaacg cgcaggagta cttcgagcgc tatcgcaagg ggcgctcggc ccaggccaat
      901 ctgccggagc tgatcgctgc cgccaccacc gaactggcct atctggacga gctgcgcacg
      961 ttgaccgagc tggccgacgg gctggacgag atcgagcaac tgcgcggcga gtgggaagag
     1021 tgggcggcgc cccagccagc ccagtcagtc cagtcagcgc gggcgcgcca gccggcccgg
     1081 ggtaagcgcc cgccgcgccc gccgcgcccc cggccctacc gcagcgagcg cggcgacctg
     1141 atctacgtcg gtcacaacgg ccggcagaac gagatcgtca cgtttgaatt ggccgggccg
     1201 gacgacacct ggctgcacgc ccgcggcatg cccggcgcgc acgtcgtcgt ccgctgggcc
     1261 ggtgcggaaa gtccggagct cctggagcag gccgccagcc tggccgcctg gtacagcgcc
     1321 ggtcgcgccg ccaccagcgt ggaggtcgac gccgccccgc gccggcacgt gcgcaagctc
     1381 aagggcgctg gccccggcat ggtcacctac cgccacgagc gcaccatcaa cgcccgcccg
     1441 cgcgcccccg aggaactggg gctggcgtag ggcagacgcg aggctcgccg acaacgtagg
     1501 ccgtcggggg gacgagggca ggcatggagg cctgccctcg ttgccctacc gccggaggtc
     1561 gtcggcgtcg cttacgccca cttggccagc aagatgccga actcgtagag cgcgtacatc
     1621 gggatggcga ccatgaacat gttgaagggg tcgggcgtcg gcgtgatgat cgccgccgcc
     1681 accatcacca cgatcagcat gtagcgccgg atatgcgcca ggaactggtg ggtgacgatg
     1741 tgcagcttgg ccagcacgta catcaccacc ggggtctcga agacgaggcc gcaccagagg
     1801 atcaacgtgg tgtagaagga gatgacctcc tccggccgca gttgcgaggt gatcgtgcca
     1861 ttgccgaagt gcgccagcca gtcgagggcg cgcggcacca cgacgaagaa ggcgaaggcc
     1921 accccgagcg cgaacatgag cacgatcagc ggcaggaagc ggtagaggac gcgcttctcc
     1981 ttgcgcgtca gacccggcgc caggaaggag acgaactggt agatcagcac cggcatggcc
     2041 agcgcgatgc cgaagtagag cccgaccttc atccaggagg tgaagctctc ggtcggcgtg
     2101 atggcgtaga actctttgac gttcgacagc cgctgcatga ggtggatgat ccgccaggcc
     2161 aggagaagcc cgaccacgga cccgaccatc accgccagcg ccgacctgac caggcgcgag
     2221 cggagctcct ccaggtgctc ctggagggtc atctcggcga attgttc
//
LOCUS       DASUBI010000319         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_899591, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000319 DASUBI010000000
VERSION     DASUBI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899591"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFU72_06090"
     CDS             <1..498
                     /locus_tag="VFU72_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5423092.1"
                     /translation="YVLLHPGASAQARRYPPERFGEVARLLGAAGQQVLLTGVEREAA
                     LLEAARAEAPEAAFLAARTTLPEYAALVARAALVVCNNSLPLHLADATGTPLVVLYSG
                     TDRESEWEPRAVPARLLRRPTACAPCRLFECPIGLPCLDIPPREVAEAALALLEPVVA
                     RVGAV"
     gene            498..1574
                     /locus_tag="VFU72_06095"
     CDS             498..1574
                     /locus_tag="VFU72_06095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5423093.1"
                     /translation="MAAIEPRAVRRIAVLRALQLGDLLAAVPALRSLRAGFPLAEITL
                     IGLPWAEQFAARFSRYLDRFVVFGGAPGIDEVELDEGRMAAYLVRQRAYGYDLAVQLH
                     GSGGASNPAVLALDARYSAGCYVGIPPTGLTVAVPYPDDLAEPLRLLRLIEAVGCPAR
                     GTQLEFPLRAGDLAEAGALLAPLDCQSGPWVGLHCGARAPARRWPAARFASLADALAR
                     RHAAAIVLTGGPGEEPIAAEVAGLMEAPALNLAGRTSLGGLAALIARLDLFISNDTGP
                     AHLARALDTPSVTIFGPAEFPRWAPLDPARHRLERQPVRCNPCAHQECPLDHRCLRGV
                     STTRVLSAAEALLSEIMRGRRCAA"
     gene            1562..>2073
                     /locus_tag="VFU72_06100"
     CDS             1562..>2073
                     /locus_tag="VFU72_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423094.1"
                     /translation="MRRLKILIWHIHGSYLNALARIEHDWYLPVKPGKPEGYGGRGPT
                     FDLPEYVREVPAEQVRDLDLDLVIYQTPKNYFEDGPAILSPAQRALPAIYLEHNTPKP
                     SAVDTRHPIDDPNLLLVHVTHYNRLMWDSGRTPTTVIEHSVAIAPGVCYTGELARGIT
                     VVNGPQKRPR"
BASE COUNT          260 a          794 c          735 g          284 t
ORIGIN      
        1 tacgtcctgc tgcaccccgg cgccagcgcg caggcgcggc gctacccgcc cgagcgcttc
       61 ggcgaagtcg cgcggctgct cggcgcggcc ggccagcagg tgctgctgac cggcgtcgag
      121 cgcgaggcgg cgctgctgga agcggcgcgt gccgaggcgc cggaggccgc cttcctggcc
      181 gcgcgcacca cactgcccga gtacgcggcg ctggtcgcgc gcgcggcgct ggtcgtctgc
      241 aataactcgc tgccgctgca cctggccgat gccaccggca ccccgctggt cgtgctctac
      301 tcgggcaccg atcgcgagtc ggagtgggag ccacgcgccg tgccggcccg gctgctgcgc
      361 cgcccgaccg cctgcgcgcc ctgccgcctg ttcgagtgcc cgatcggcct cccctgcctc
      421 gacatcccgc cgcgggaggt ggccgaggcc gcgctggcgc tgctcgagcc ggtggtcgca
      481 cgagtgggag ccgtctgatg gccgctatcg agccgcgcgc ggtgcggcgc atcgccgtgt
      541 tgcgggcgct gcagttaggc gacctgctgg cggcggtgcc ggcgctgcgc tcgctgcgcg
      601 ccggctttcc cctggccgag atcaccctga tcggcctccc ctgggcggag cagttcgccg
      661 cccgcttcag ccgctacctc gaccgcttcg tcgtctttgg cggtgcgccg gggatcgacg
      721 aggtcgagct ggacgaggga cgcatggcgg cctatctggt ccggcagcgc gcctacggct
      781 atgacctggc ggtgcagctc cacggcagcg gcggcgccag caaccccgcc gtgctggcgc
      841 tcgacgcgcg ctacagcgcc ggctgctacg tgggcattcc gcccaccggg ctgactgtgg
      901 cggtgcccta ccccgacgac ctcgcggagc cgctgaggct gctgcgcctc atcgaagccg
      961 ttggttgccc cgcgcgcggc acgcagctgg aattcccgct ccgggcagga gacctggccg
     1021 aggccggtgc gctcctggcg ccgctggatt gccagagcgg gccgtgggtc gggctgcact
     1081 gcggcgcgcg tgccccggcc cgtcgctggc ccgccgcgcg cttcgccagc ctggccgacg
     1141 ccctggcgcg ccgtcacgcc gccgccatcg tgctgaccgg cgggccgggc gaggagccga
     1201 tcgcggccga ggtggccggg ctgatggagg ctccggcgct caacctggcc gggcgcacct
     1261 cgctgggtgg gctggcggcg ctgatcgccc ggctcgacct cttcatcagc aacgataccg
     1321 gcccggcgca cctggcccgc gcgctcgaca cgcccagcgt caccatcttc ggcccggccg
     1381 agttcccgcg ctgggcgccg ctcgacccgg cgcgccaccg gttggagcgg cagccggtgc
     1441 gctgcaaccc ctgcgcccac caggagtgcc cgctcgacca ccgctgcctg cgcggggtga
     1501 gcacgacgag ggtgctgagc gccgccgagg cgctgttgtc cgaaatcatg agaggaagac
     1561 gatgcgccgc ctgaagatcc tgatctggca catccacggc agctatctca acgcgctggc
     1621 gcgcatcgag cacgactggt acctgccggt caagccgggc aagcctgagg gctacggcgg
     1681 gcgcgggccg accttcgatc tgccggagta cgtgcgcgag gtgcccgccg agcaggtgcg
     1741 cgacctcgat ctcgacctgg tgatctacca gacgcccaag aactacttcg aagacggtcc
     1801 ggccatcctc agcccggcgc agcgcgcgct accggcgatc tacctggagc acaacacgcc
     1861 caagccgagc gccgtcgaca cgcgccaccc gatcgacgac ccgaacctgc tgctggtcca
     1921 cgtcactcac tacaaccgcc tgatgtggga cagcggccgc acgcccacca ccgtcatcga
     1981 gcacagcgtg gccatcgcgc cgggcgtgtg ctacacgggc gagctggcgc gcggcatcac
     2041 cgtcgtcaac ggcccccaga agcggccgcg cat
//
LOCUS       DASUBI010000320         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_19107, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000320 DASUBI010000000
VERSION     DASUBI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19107"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFU72_06105"
     CDS             complement(<1..826)
                     /locus_tag="VFU72_06105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5423095.1"
                     /translation="MLPVPKRSPARRWTGRIAGSPVALALGLGLAAALLYLSRRSISL
                     DDFDSVSFALALRDFNLFVQQPQPPGFPVYVALGRALLRLHADPRWALVTLSALSGGA
                     AVSATFGLGYLLSGGRRAAGLAAALLLGLMPLFWLSSEKALSDVPALAGLLVAILLLI
                     LARERATAGWAALAGLAAGLSLGFRPQNVLALLPFALALALVLARGRRWRGLAALGAS
                     GLAGVALWLAPVVEISLGPSLYLQLLRIQGRHVWQTDSLFAAGPLSLGTVHARLSDV"
     gene            975..1328
                     /locus_tag="VFU72_06110"
     CDS             975..1328
                     /locus_tag="VFU72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5423096.1"
                     /translation="MRRDEVLRILAQHHAELAEMGVGSLALFGSVARDKAGPESDVDL
                     LVEFRKPVGLFELVGLKEYLEQLLGRPVDLVPRDSIKRQSRERILQEAVPAYYRCLRH
                     GSTPLVLVLDLGETA"
     gene            1583..2845
                     /locus_tag="VFU72_06115"
     CDS             1583..2845
                     /locus_tag="VFU72_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEU5423097.1"
                     /translation="MSHHDGHRYDVAVVGAGPGGAATAAYAAEMGLDVLLLDRASFPR
                     DKTCGDGLTPRAQRVLAEMGLLGEALGAGHRLNTMEIVAPSAYRATTPMLRSDARTDG
                     VAVVPRLILDELIVRRAEARGARLETPVRVVDVVQEGAGGEGVLLTAERQGREVEYRA
                     RMAVIATGAGMGLLRRIGLLEQPPRLLMLCGRAYFSDISLPLEEIGVHFAGVTLPGYG
                     WVFPLDGGRANIGAGVFRPGRKASGPQNGQQAFARFVASPHLGRALAGARREGPVRAA
                     PLRLDFATAPTYGPRTLLVGEAAGLVSPVTGEGIDYALESGKIAAEHLHAMLADGDCS
                     DERLRAYDRELRRRYQRLFVLFNRLRWLFLNPRVLTKLVVAADRHPDLMDLCLSIVLE
                     NENVADAFRARTVLKVLLTRAPAAASRA"
BASE COUNT          448 a         1044 c         1049 g          399 t
ORIGIN      
        1 ggacgtcgct caggcgcgcg tgcacggtgc ccaggctcag cggcccggcc gcgaagagcg
       61 agtcggtctg ccagacgtgc cgcccctgga tgcgcagcag ctgcaggtag aggctgggcc
      121 cgagcgagat ctccaccacc ggcgccagcc agagcgccac cccggccagc ccgctcgcac
      181 cgagcgccgc cagcccgcgc cagcggcgac cgcgtgccag caccagcgcc agggcgagcg
      241 cgaaggggag gagcgccagc acgttctgcg gccggaatcc gaggctgagt ccggcagcga
      301 gccccgccag tgccgcccag cccgccgtgg ctcgctcccg cgccaggatg aggaggagga
      361 tggcgacgag cagccccgcc agcgccggaa cgtcggagag cgccttctcg ctgctgagcc
      421 agaagagcgg catcagtccc aagagaagcg cggcggcgag cccggcggca cgccgcccac
      481 cgctcagcag gtagcccagc ccgaacgtgg ccgacaccgc cgccccgccg ctcagcgcgc
      541 tgagtgtgac cagcgcccag cgcgggtcgg cgtgcagccg gagcagggcg cgccccaggg
      601 cgacgtagac cggaaagccg ggcggctgcg gctgctgcac gaagaggttg aagtcgcgaa
      661 gggccagggc gaagctgacc gagtcgaagt cgtccagcga gatgctgcgc cggctgagat
      721 agaggagcgc ggcggccagc ccgagaccca gggcgagcgc gaccggcgag cccgcgatcc
      781 gccccgtcca gcgccgagct ggcgagcgct tcggaaccgg gagcaccgtc gtcaagccga
      841 aagacccttc tacattccaa ggatgcgatc cggcgcaact ctaccagccg gcacgcgcgt
      901 cgcggcggaa tcggccctgc tagactgggg cgagatttgg gaccgcgcac gaggaacgcc
      961 tagaggagcg gggtatgcgg cgcgacgagg ttctccgcat tctggcccaa catcacgccg
     1021 aactggcgga gatgggcgtc ggctctctcg cgctcttcgg ctcggttgcg cgcgacaaag
     1081 ccggaccgga gagcgatgtc gatctgctgg tcgagttccg caagccggtc gggctcttcg
     1141 agttggtggg actgaaagag tacctggagc agctgctcgg tcgtccggtc gatctggtgc
     1201 cgcgcgacag catcaaacgt cagtcgcgcg agcgcattct ccaggaggcg gtccctgcct
     1261 actatcgatg cttacggcat ggatcaacgc cgctcgttct cgtgctggat ctcggagaga
     1321 ccgcctgatg tttgaggaaa cgctgtggct cgcgtcgtgc ccaaactcag ccccttcagt
     1381 gggcttggtg gattcagcgc ggcgagttta ctcccgcgcg tcaggtggca cggagcccag
     1441 ccgaaatgcg tcagttgacg ccccagcggc gcgcttgcta gcgtaggcga acgcgggccg
     1501 agtgacgtcg acggcgcgga tcgtgccggc acgtgaccgg atcagcctcc agctcgggta
     1561 cgcatggagg gagcgacggg aaatgagcca ccatgacggg catcgctacg acgtggccgt
     1621 cgtcggcgcc ggccccggcg gcgccgccac ggccgcctac gccgcggaga tggggctcga
     1681 tgtcctcctc ctcgaccgcg ccagcttccc gcgcgacaag acctgcggcg acggcctgac
     1741 tccgcgcgcc cagcgcgtgc tcgccgagat gggtttgctc ggcgaggccc tgggcgccgg
     1801 acaccgcctg aacacgatgg agatcgtggc gccaagcgcc taccgggcca ccaccccgat
     1861 gctccggagc gacgcgcgaa ccgacggcgt ggccgtcgtg ccgcgcctga tcctcgatga
     1921 attgatcgtg cgccgcgccg aggcgcgtgg cgcgcggctg gagaccccgg tgcgcgtcgt
     1981 cgatgtggtg caggagggag cggggggcga gggcgtgctg ctgaccgccg agcggcaggg
     2041 gcgggaggtc gagtaccgcg ctcgcatggc agtcatcgcc accggggccg gcatggggct
     2101 gctgcgccgc atcggcctgc tggagcagcc gccacgcctc ttgatgctct gcggccgcgc
     2161 ctacttcagc gacatctcgc tcccgctgga ggagatcgga gtccacttcg ccggggtgac
     2221 gctgcccggc tacggctggg tcttcccgct cgacggcggc cgcgccaaca tcggcgcggg
     2281 ggtcttccgt cccggtcgca aggcgagcgg gccgcagaac gggcagcagg ccttcgcccg
     2341 cttcgtggcc tcgccgcacc tcggccgcgc gctcgccggc gcccgccgcg aaggcccagt
     2401 cagggccgca ccgctgcgac tcgacttcgc caccgccccg acctacgggc cacgcacgct
     2461 gctggtgggc gaggcggccg gtctggtcag tccggtgacc ggcgagggga tcgactacgc
     2521 gctggagtcg ggcaagatcg ccgccgagca cctgcacgcg atgctggccg acggcgactg
     2581 ctcggatgag cggctgcgcg cctacgaccg cgagctgcgc cggcgctacc agcgcctctt
     2641 cgtcctcttc aatcgcctgc gctggctctt cctcaacccc cgggtgctga ccaagctggt
     2701 cgtcgccgcc gaccgccacc ccgacctgat ggacctctgt ctgagcatcg tgctggagaa
     2761 cgagaacgtc gccgacgcct tccgcgcccg caccgtcctc aaggtgctgc tgacccgcgc
     2821 tcccgccgcg gcgtcgcgcg cttgacatca aagatcgccg cttgataccc tctgcgggtc
     2881 gtgctgcggc gaaagccgtt cgagaggtgt gcctgctgat gctctaccgt atgcccggac
//
LOCUS       DASUBI010000321         9295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_806148, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000321 DASUBI010000000
VERSION     DASUBI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806148"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFU72_06120"
     CDS             complement(<1..931)
                     /locus_tag="VFU72_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5423098.1"
                     /translation="MVHIDKDARSNASVSPAVRDPENPPLPAQGVRQPFSPEQTSGLA
                     SYPPPERWDDWEEFDPRAWPKPVKHRYMLVPTTCFNCESACGLMAYVDRETLEVRKLE
                     GNPFHPGSRGRNCAKGPATLNQIEDPDRILYPLKRVGPRGAGQWERVSWDEVLDTIAA
                     KIRGALTSGRQNEVMYHVGRPGEDGYMERVLQSWGIDGHNSHTNICSSGGRTGYAFWM
                     GFDRPSPDFANARFILLVSAHLESGHYFNPHAQRIMEAKANGAKLAVIDPRLSNTASM
                     ADYWLPAAPGTEAALLLGIAHVMLRDGLCDLDFV"
     gene            complement(988..2523)
                     /gene="nrfD"
                     /locus_tag="VFU72_06125"
     CDS             complement(988..2523)
                     /gene="nrfD"
                     /locus_tag="VFU72_06125"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5423099.1"
                     /translation="MRYGFVIDNRKCIGCHACTTACKSENEVPLGVFRTWVKYTEKGT
                     FPNTRRYFNVTRCNHCDNAPCITVCPTKALYRRPDGIVDFDSSRCIGCKSCMQACPYD
                     ALYIDPFEHTAAKCNYCAHRVDVGLLPACVIVCPEKAIIAGDLDDPTSEIAHLVATQP
                     VAVRKPEQGTQPKLFYIDADAAALTPEAQTRQDGYLWGEVRPDDRPRLAVTAGDGPDH
                     AAEARIAYDVHHPKPWGGKVATYLWTKSIGAGAALVAAILFLAGAVDNRATFEIAAPL
                     IAILFILATTVLLVADLKRPERFWYLLLKPNPRSWLVWGGYILGAFGAVAFLWLVVAA
                     AGLDTATKVLMALSIPLALAAAGYTAFLFGQAEGRDFWQSRLLLPHLIAQAIVAGAAA
                     LLLFGVSLGASNHELRVTSVALAVGLVLFAGLLLAELRGAHSNAHVARTVKLLTEGPY
                     RPVLLYGVWLLGVLAPLLLIAATLLTGLEIVTAAFAASAALAGLWFWERIWVSAGQDV
                     PLS"
     gene            2874..4757
                     /locus_tag="VFU72_06130"
     CDS             2874..4757
                     /locus_tag="VFU72_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5423100.1"
                     /translation="MLIHSHQWRARAVALITGAYLLYYLWWRTTATLNLDALVFSLVF
                     LLAELQGALSYALFAFMTWDCERQFAFEPLPGRAVDIFIPTFNEPLDILEATLVGCGS
                     IRYPHTTYVLDDGRRPEVAALAASLGCRYLTRENNLHAKAGNLNAALARTDGEFVVIL
                     DADTVPQPDFVDKTLGYFVDERVALVQLPQEFYNADSIQHAGRRGEPDPWHDQALFYR
                     VIQPGKNRWNAAFWCGSPAIVRRTALDSVGGVATGSITEDIHTSLRLHSAGWKTIYHN
                     ETLAYGIAPQTLHAFALQRLRWAQGTMQILRSRENPLLKPHLTLAQRLNYLASMLTYF
                     DAFQKLIYLVVPALILLSGVLPLTVSGWIYLAHWLPYFALGILANKALGRGSFRYLRV
                     EEYNLLKMFTFIWASSVIVWPRRLGFRVTPKETADGVQAQERRQVAPLALLLGIIAGT
                     VGLALINLLFGLYRIDLAQSIYLVAIVWALVNAGILGGCLYTVLHRVHARKRYRFPAH
                     VPAVVLGADGSLLETTTSDLSLEGMGLLIPADQLWLSPRVELLLALPDGDAVITGDIL
                     HARSRPRGRCRVGVRFREMNAETRRRLIGYLFVTLPRGTATDQPALPLAPFVELQSAP
                     AAA"
     gene            4878..6071
                     /locus_tag="VFU72_06135"
     CDS             4878..6071
                     /locus_tag="VFU72_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU5423101.1"
                     /translation="MRDTGQWLVGAAGLFLIFSVLLDAFETIVLPRRVTRQFRLTRLV
                     YRLTWIPWSSVARHMSRHSPRRESFLSFYGPLSLIILLLVWVVTLVVGFGLLFWGLGS
                     PLVLARGREHLSTDIYLSGTNFITLGIGDVDPNTTLTRAITVIEAATGFGFFALVISY
                     LPTLYQAFSRREARIALLDARAGSPPTAVELLRRAGRSHGVAKLDQFLYSWEEWSAEL
                     LESHLSYPTLAYFRSQHENQSWLGALTMILDACSLVLVGVDGVPAGQAQLTFAMARHA
                     AVDLSQVFGARPEPPSPPRLTVDEFTAIQAELADSGLPMPKGAEAWHELGQLRELYEP
                     YVNALARRLLFALPSWLPPAVVHDDWAASAWESVWAIPPLPDFENEAGWLISEETRPG
                     PAADG"
     gene            6109..6405
                     /locus_tag="VFU72_06140"
     CDS             6109..6405
                     /locus_tag="VFU72_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEU5423102.1"
                     /translation="MVRGPNNMHGEYKVPGGKLVVADLEIRDGRFAAVEISGDFFLEP
                     AEALALIAEAITGLPADLTAAEIAEQISNALPDDIVMLGFSPEAVAHAVRRAVG"
     gene            6402..7178
                     /locus_tag="VFU72_06145"
     CDS             6402..7178
                     /locus_tag="VFU72_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEU5423103.1"
                     /translation="MIERDWTAYPWQLIRGEAIEPVLNHAIDEVLARRVGAGARPPTL
                     RLWGRNAPEVVIGRFQSVRNEVDEEAARRHGIAINRRLTGGGAMFVEPANIITYSIYA
                     PPELVDGLSFVDSYAFLDSWVIEALRALGIDAWYEPINDITSSGGKIGGAAQARRFGS
                     VLHHATMSYDLNSDRMLEVLRIGKEKLSDKGIASAAKRVGPLRQQTNLPRETVVSHML
                     QTFAARHGLNESELTPEELAEARDLVATKYATPEWIYILP"
     gene            complement(7429..9138)
                     /locus_tag="VFU72_06150"
     CDS             complement(7429..9138)
                     /locus_tag="VFU72_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU5423104.1"
                     /translation="MSLSTAGVLSGTAATGSGGVYPIEITASNGVSPDATQAFTLTVD
                     EAAAFTSADQTIFTVGNAGSFSVTTTGFPAPSLSEIGSLPAGVTFADNGDGTATFSGT
                     PAVGSAGSYPLTITARISGQGDVSQASQSFTLSVDAAPVADAGGPYTVAEGGNVTLDA
                     DQSSDPDGDTLTYSWDLNGDGAFGDATGVNPTLTWAQLEALGLSDGPAAISNVTVRVD
                     DGHGHTVISPATGLTIENVPPQGSISGGQSLGGSPGQPLSVTFSASDPSPQDHAAGFS
                     FAIDWGDGSGVQTVKGTSPQAVSHTYGGVGVYRISMTATDKDGGTKGAAFASVTIASA
                     RIVPDPCDAGSTALMVSGTSGNDSISISPARGVRQVAVTLNGVPQGTFGPFSRIIVYG
                     QAGDDSITVERGIAQPSIIYGGDGDDTIRGGNGTGILIGGNGDDDLTGGGVSDILIGG
                     SGADTLVGGSGEDILIAGSTSYDEAASGNQAVLCAISAEWSHPLVSYHTRIDHLTGAA
                     TGGLNGTSYLLGPGAGQTVFNDVSPDTLTGGFGRDWYLLNEAGGGALDMSNRTLLEVA
                     TDL"
     gene            9125..9265
                     /locus_tag="VFU72_06155"
     CDS             9125..9265
                     /locus_tag="VFU72_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423105.1"
                     /translation="MDKLTPLGSEPVALKLGAGEPVAVSVKRSACPTLKVVLLALVIA
                     GA"
BASE COUNT         1413 a         3221 c         3124 g         1537 t
ORIGIN      
        1 gcacgaagtc gaggtcgcag aggccgtcgc gcagcatgac gtgggcgata cccaagagca
       61 gcgccgcctc ggtgccgggc gcggccggca gccagtagtc ggccatcgag gcggtgttgg
      121 agaggcgcgg gtcgatcacc gccagcttgg cgccattggc cttggcctcc atgatccgct
      181 gcgcgtgcgg gttgaagtag tggccgctct ccaggtgcgc gctgaccaac aggatgaagc
      241 gcgcattggc gaagtcgggc gaggggcggt cgaagcccat ccagaaggcg tagccggtcc
      301 ggccgcccga ggagcagatg ttggtatgcg agttgtggcc gtcgatgccc cacgactgca
      361 gcacgcgctc catgtagccg tcctcgccgg ggcggcccac gtggtacatc acctcattct
      421 ggcgcccgct ggtcagcgcg ccccggatct tggcggcgat ggtgtccagc acctcgtccc
      481 agctcacccg ctcccactgg cccgccccgc gcgggccgac acgcttgagc gggtagagga
      541 tgcggtcggg gtcctcgatc tggttgagcg tggccgggcc tttggcgcag ttgcgcccgc
      601 ggctaccggg gtggaagggg ttgccctcca gcttgcgcac ctccagggtc tcgcggtcca
      661 cgtaggccat cagcccgcag gccgactcgc agttgaagca ggtggtcggc acgagcatgt
      721 agcggtgttt gaccggtttg ggccaggcgc gcgggtcgaa ctcctcccag tcgtcccagc
      781 gctcgggcgg cgggtaggag gccaggccgc tggtctgctc cggactgaag ggctggcgca
      841 cgccctgcgc cgggagcggt gggttctcgg ggtcgcgaac cgcgggcgag acgctggcgt
      901 tgctgcgagc atccttgtcg atgtggacca tgaatccctg aatccttact ccctagcgcg
      961 cgaatagtcg aatgagcgcg cgagcgctca gctcagcggc acgtcctggc cggcgctcac
     1021 ccagatccgc tcccagaacc agagtccagc cagcgcggcg ctggcggcga aggcggcggt
     1081 cacgatctcc agcccggtca ggagcgtggc ggcgatcagg agcagcgggg ccagcacgcc
     1141 gagcagccaa acgccataga gcagcaccgg gcggtacggc ccctcggtta gcagcttcac
     1201 ggtgcgggcg acgtgcgcgt tggaatgcgc cccgcgcagc tccgccagca gcagcccggc
     1261 gaagagcacg agacctaccg ccagcgcgac cgacgtgacg cgcaactcgt ggttgctggc
     1321 gccgagcgac acgccgaaga ggagcagcgc cgccgcgccg gccacgatcg cctgcgcgat
     1381 cagatgcggc aagagcaggc gcgactgcca gaagtcccgc ccctcggcct ggccgaagag
     1441 gaaggcggtg tagcccgcgg ccgccagcgc cagcgggatg gacaacgcca tcagcacctt
     1501 cgtggcggta tcgagcccgg cagcggccac caccagccag aggaaggcca ccgcgccgaa
     1561 ggcgcccagg atgtagccgc cccagaccag ccaggagcgc gggttcggct tgagcagcag
     1621 gtaccagaag cgctccggcc gcttgagatc ggccaccagc agcaccgtag tcgccaggat
     1681 gaagaggatc gcgatcaatg gtgcggcgat ctcgaaggtg gcgcggttgt cgaccgcgcc
     1741 ggccaggaag aggatggcgg cgaccagcgc ggccccggcg ccgatcgact tggtccagag
     1801 gtaggtcgcc accttgccgc cccacggctt ggggtggtgc acgtcgtagg cgatgcgggc
     1861 ttccgcggcg tgatcgggcc catcgcccgc ggtcaccgcc agccggggcc ggtcgtcagg
     1921 ccgcacctcg ccccaaaggt agccgtcctg ccgcgtctgc gcctcgggcg tcagcgcggc
     1981 cgcgtcggca tcgatgtaaa agagcttggg ctgcgtgccc tgctccggct tgcgcaccgc
     2041 taccggctgg gtcgcgacca ggtgcgcgat ctcgctcgtc gggtcgtcca gatcgccagc
     2101 gatgatcgcc ttctccgggc agacgatgac acaggccggc aacagcccga cgtcgacccg
     2161 gtgcgcgcag tagttgcact tggcggcggt gtgctcgaag gggtcgatgt agagcgcgtc
     2221 gtaggggcag gcctgcatgc acgacttgca gccgatgcag cgcgaggagt cgaaatcgac
     2281 gatgccgtcc ggccggcgat agagcgcctt ggtcgggcag acggtgatgc agggcgcgtt
     2341 gtcgcagtgg ttgcagcggg tgacgttgaa gtagcgccgg gtgttgggga acgtcccctt
     2401 ctcggtgtac ttcacccagg tgcggaagac gcccagcggc acctcgttct cgctcttgca
     2461 ggcggtggtg caggcgtggc agccgatgca cttgcggttg tcgatgacaa acccgtatct
     2521 catgccgaaa cgcacttcca gttcttgtgc acttgtgctc ttgtgctctt ggcctcgggc
     2581 gaacggatcg cgggcgcacc gtaccaaagg cgcgggcgcc tgtccagcca gcccgtgcgt
     2641 tggcgcgtcg tcatgcgagc ccgggtacac cgaattcctc cgcgcacggg gcacgtcact
     2701 ggtcttccgc cgccgcgcag caggctcgtg tcgcgagtca atcgggactc cacgcccgaa
     2761 ctgaccggta cctcagcctc atcacgtacc cgcgcattcc cctgcaagct tcagacacgc
     2821 cagcggcggc gcgtgtgccg taggcgcaac gatgagaggc gagtgccggt tcgatgctca
     2881 tccattctca ccagtggcgc gctcgcgccg tcgccctgat caccggggcc tatctgctct
     2941 actatctctg gtggcgcacc accgccacgc tcaatctcga cgcgctcgtc ttctcgctcg
     3001 tctttctctt ggcggagctg caaggcgcgc tgagctacgc cctattcgcc ttcatgacct
     3061 gggactgcga gcggcagttc gccttcgagc cgctgcccgg acgcgccgtc gacatcttca
     3121 tccccacctt caacgagccg ctggatatcc tggaggcgac gctggtcggc tgcggttcga
     3181 tccgctaccc gcacacgacc tacgtgctcg acgacggccg ccgtcccgag gtggccgcgc
     3241 tggccgcctc gctcggctgc cgctacctga cgcgcgagaa caacctgcac gctaaggcgg
     3301 gcaacctcaa cgccgcgctg gcgcgcacgg acggcgagtt cgtggtgatc ctcgatgccg
     3361 acaccgtgcc ccagcccgac ttcgtcgaca agacgctggg ctacttcgtg gacgagcgcg
     3421 tggcgctggt gcagctcccg caggagttct acaacgccga ctcgatccag cacgcggggc
     3481 ggcgcggcga gccggacccc tggcacgacc aggcgctctt ctaccgcgtc attcagccgg
     3541 gcaagaaccg ctggaacgcg gccttctggt gcggcagccc ggccatcgtg cggcgtaccg
     3601 cgctcgactc ggtcggcggc gtggcgaccg gctcgatcac cgaggacatc cacacctcgc
     3661 tgcggctcca ctcagcgggc tggaagacga tctaccataa cgagacgctg gcgtacggca
     3721 tcgcgccgca gacgctgcac gccttcgcgc tgcagcgcct gcgctgggcg caggggacca
     3781 tgcagatcct gcgcagccgc gagaacccgc tgctcaagcc gcacctgacg ctggcccagc
     3841 ggctcaatta cctggcctcg atgctgacct acttcgacgc cttccagaag ctgatctacc
     3901 tggtcgtgcc ggcgctgatc ctgctgagcg gcgtcctgcc gctgacggtg agcggctgga
     3961 tctacctggc gcactggctg ccctacttcg cgctcggcat cctggccaac aaggcgctcg
     4021 gccgcggcag cttccgctac ctgcgcgtcg aggagtacaa cctgctcaag atgttcacct
     4081 ttatctgggc gtccagtgtg atcgtctggc cgcggcggct gggcttccgc gtcacgccga
     4141 aggagaccgc cgacggcgtc caggcgcagg agcggcgcca ggtggcgccg ctggccctgc
     4201 tgctcggcat catcgccggc acggtcggcc tggccctgat caacctgctg ttcgggctgt
     4261 accgcatcga tctggcccag agcatctacc tggtggccat cgtgtgggcg ctggtcaacg
     4321 ccggcattct gggtggctgt ctctacaccg tgctgcaccg cgtccacgcg cgcaagcgct
     4381 accgcttccc ggcgcacgtg cccgccgtcg tgctcggcgc cgacggcagc ctgctggaga
     4441 cgaccaccag cgatctctcg ctggagggca tggggctgct cattccagcc gaccagctct
     4501 ggctgtcgcc gcgggtggaa ctcttgctcg cgttgccgga cggcgacgcg gtcatcaccg
     4561 gcgacatcct gcacgcgcgc agccggccgc gcgggcgctg ccgggtcggc gtgcgcttcc
     4621 gcgagatgaa cgccgagacg cgccgccgtc tgatcggcta cctcttcgtg acgctcccgc
     4681 gcgggaccgc gaccgaccag ccggcgcttc ccctggctcc cttcgttgaa ctccagtccg
     4741 caccggccgc cgcctgaact ccccgccggc aacgatatga ccgggcaccc gccgcacgcg
     4801 gtaggctttg acgcggcccg cttgggggac acgcagggtc gaaagcctct ggctgtgggc
     4861 aaaggcggtg ggcggggatg cgggacacag ggcagtggct ggtcggcgcg gccgggttat
     4921 tcctgatctt ctcagttctc ctcgatgcct tcgagacaat cgtgctgccg cgccgcgtca
     4981 cccggcagtt tcgcctgacc cgcctggtct accgcctgac ctggattccc tggtccagcg
     5041 tcgcccgcca catgagccgg cacagccccc ggcgggagag cttcctcagc ttctacggtc
     5101 ccctctcgct cattattctc ctgctggtct gggtggtaac gctcgtggtc ggcttcggtc
     5161 tcctcttttg gggacttggc tcaccgctcg tgttggcacg cggccgtgaa cacctgagca
     5221 ccgatatcta cctcagcggg acgaacttca tcaccctggg tatcggtgac gtcgatccga
     5281 atacgacgct gacgcgagcc atcacggtga tcgaggcggc caccgggttc ggcttcttcg
     5341 ccctggtaat cagctatctg ccgacgctgt accaggcgtt ctcgcgccgc gaggcgcgca
     5401 tcgcgctgct cgacgcccgc gctgggtcgc cgccaaccgc ggtcgagctg ctgcggcgcg
     5461 ccgggcgctc tcatggcgtg gcgaagctcg accagtttct ctacagctgg gaagagtggt
     5521 cggccgagct gcttgagagc catctctcct acccgacgct ggcgtacttc cgctcgcagc
     5581 acgagaatca atcctggctc ggggcgctca ccatgatcct ggacgcctgc tcattggtgc
     5641 tggtgggtgt cgatggcgtg ccagccggac aggcgcagct gaccttcgcc atggcacgcc
     5701 atgcggccgt ggacctcagc caggtcttcg gtgcccggcc cgagccaccg tcgccgccac
     5761 gcctgaccgt ggatgagttc actgccatac aggccgagct cgccgactcg ggattaccga
     5821 tgccgaaagg agccgaggcc tggcacgaac tcgggcagct tcgcgagctg tatgaaccct
     5881 acgtcaacgc actggcccgc cgcctcctct tcgccttgcc gagctggctg ccgccggcgg
     5941 tggtgcatga cgattgggcc gcgtcggcct gggagtcggt ctgggctatc ccgccgctac
     6001 ccgatttcga gaacgaagcc ggctggctga tctccgagga gactcgaccg ggaccggccg
     6061 ctgacggata attgagctgc gcggctccga gcgagagcgg atcgagagat ggtgcgaggt
     6121 ccaaacaata tgcacggtga gtacaaggtg ccgggcggca agctggtggt cgccgacctg
     6181 gagatccgcg acggtcgctt cgccgcggtc gagatcagcg gcgacttctt cctggagccg
     6241 gccgaggcgc tggcgctgat cgccgaggcg atcacggggc tgccggctga cctgacggca
     6301 gcggagatag cggagcagat cagcaacgcc ctgccggacg acatcgtcat gctcggcttc
     6361 tcgccggagg cggtcgccca cgcggtgcgg agggctgtgg gatgatcgag cgcgactgga
     6421 cggcctatcc ctggcaactg atccgcggcg aggcgatcga gccggtgctc aaccacgcca
     6481 tcgacgaggt gctggcgcgc cgcgtcggcg ctggcgcgcg cccgcccacc ttgcgcctct
     6541 ggggccgcaa cgcacccgag gtcgtcatcg gccgcttcca atcggtgcgc aacgaggtgg
     6601 acgaggaagc tgcgcggcgg cacggcatcg cgatcaaccg gcggctaacc ggcggcggcg
     6661 ccatgttcgt cgagccggca aatatcatca cctactcgat ctacgccccg cccgagctgg
     6721 tcgacggcct ctcgttcgtc gactcctacg ccttcctcga ctcctgggtg atcgaggcgc
     6781 tgcgcgcgct ggggatcgac gcctggtacg agccgatcaa tgacatcacc tcgtccggcg
     6841 gcaagatcgg cggcgccgcc caggcgcgcc gcttcggcag cgtgctgcac cacgccacca
     6901 tgtcgtacga cctcaactcc gaccgcatgc tcgaggtgct gcgcatcggc aaggagaagc
     6961 tgagcgacaa gggcatcgcc agcgcggcca agcgcgttgg ccccctgcgc cagcagacga
     7021 acctgccgcg cgagacggtc gtctcacaca tgctgcagac cttcgccgcc cgtcacggtc
     7081 tcaacgagag cgagctaact cccgaggaac tcgctgaggc gcgcgatctg gtcgccacca
     7141 agtacgccac ccccgagtgg atctacatcc tgccgtagcg ctcgggccgc accggcgcgc
     7201 gtcgaccggg ccgagtgccg ggtgaggatg gatccctcgt ccgcgctcgg gaagacaggg
     7261 tcactggcgg gctgacgacg aaggtttgcc tcgtccggca ggccacctct ctcaccctgc
     7321 catcctgagc gcggtcgggg gatccgtcgt ggagcgatac cgatcggtgt cgatagctac
     7381 agacgccccg agggaaatcg accaggacgt ctgttgtgtg ccgcgccctc agagatccgt
     7441 cgccacctcc agcaaggtgc ggttcgacat gtccagcgct cccccacccg cctcgttcag
     7501 caggtaccaa tcgcgaccga agccaccggt gagcgtgtcg ggggagacat cgttgaagac
     7561 cgtctggccc gcgcccggcc ccagcaggta gctcgttccg ttcaggccgc cggtcgcggc
     7621 tcctgttaga tgatcgatgc gcgtgtgata gctcaccaac gggtgcgacc actcagccga
     7681 gatggcgcac aagaccgcct gattcccact cgccgcttcg tcgtagctgg tgctgccggc
     7741 gatcaggata tcttcgccgc tgcctccgac cagcgtgtcc gcgccactgc cgccgatcag
     7801 gatatcgctc acgccgccgc cggtgaggtc gtcgtcgcca ttacccccga tgagaatacc
     7861 ggtgccgttg ccaccgcgga tggtgtcgtc gccgtcacca ccgtagatga tgctcggctg
     7921 cgcgatccct cgctccacgg tgatgctatc gtcaccggcc tgaccataga cgatgatgcg
     7981 actgaaaggc ccgaacgtgc cttgcgggac tccgttgagc gtcaccgcga cctggcgcac
     8041 ccctcgggcc gggctgatcg agatgctgtc gttgccggag gtgccactga ccatcagtgc
     8101 ggttgagccg gcgtcacacg ggtcagggac gatccgtgcg ctggcgatag tgaccgaggc
     8161 aaacgcggcc cccttcgtgc cgccgtcttt gtcggtcgcc gtcatggaga tcctgtagac
     8221 tccgaccccg ccgtaggtat ggctcacggc ctgggggctc gtgcccttga cggtctgcac
     8281 cccacttccg tcaccccagt cgatcgcaaa gctgaacccg gcagcgtgat cctgcgggga
     8341 cgggtcgctt gcgctgaacg tgacgctgag cggctgaccc gggctcccgc ccaggctttg
     8401 cccgccggag atactcccct gcggaggtac attctcgatg gtgaggccgg tggccgggga
     8461 gatcacggtg tgaccgtggc catcgtctac tcgaaccgtg acgttgctga tcgccgccgg
     8521 tccgtcactg aggcccagcg cctcaagctg cgcccaggtg agcgtcgggt tgacgcccgt
     8581 cgcgtcgccg aaggccccat cgccattgag gtcccaggaa taggtcagcg tgtcgccatc
     8641 cggatcggac gattgatcgg catcaagcgt cacgttgccg ccctcggcga cggtgtacgg
     8701 ccctccggcg tcggccaccg gcgcggcatc gacagagagc gtaaaactct ggctggcctg
     8761 gctcacgtcc ccctggccac taattcgcgc cgtgatcgtg aggggatagc tgccggcgct
     8821 gccgacagcg ggcgtgccgc tgaacgtcgc ggtgccgtcc ccgttgtccg caaacgtcac
     8881 gccagccggc agcgacccga tctcgctcag gctcggggcc gggaatccgg tcgtcgtgac
     8941 gctgaacgag ccggcgttgc ccaccgtgaa gatcgtctga tccgcgctgg tgaacgccgc
     9001 cgcttcatcc accgtcagcg tgaaagcctg cgtggcgtcg gggctcacgc cgttactggc
     9061 ggtgatctca atcgggtaga ccccgccgct gcccgtggcc gccgtcccgc tcagcactcc
     9121 ggccgtggac aagctcacgc cgctggggag cgagccggtc gcgctgaagc tcggggctgg
     9181 ggagcccgtc gccgtgagcg tgaagaggtc agcctgtccg accctgaagg tcgtgctgct
     9241 cgcgctggtg atcgccggtg cctgatcgac cgcgtccgag gtgccgatga cggtg
//
LOCUS       DASUBI010000322        11150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_465331, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000322 DASUBI010000000
VERSION     DASUBI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465331"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VFU72_06160"
     CDS             complement(<1..1199)
                     /locus_tag="VFU72_06160"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEU5423106.1"
                     /translation="MPYAGPLFLAAALGLVVGAVVQRRRDAPGSGALLLLVASGSLWA
                     AAYGAAYLSAGMGAKLFWAKLAYSGAAWIPFAWLIFAFAYTRRAGWLTRRALLLLAIE
                     PLVTVVLLWTNRSHHLYWADVEQSGAEHFSIFSPGLWFWAHLAYSYALLALASLPLLR
                     VLNHSLRLFRRQAAALLIVILVPWLANLLTVMPLTPASWPDLTPFAFPIVELLLAWNL
                     ARWHLLDVVPVARDLSVQGMSDGIVVVDRTARVVDVNPAAQRMLGWSSDALGQPLAER
                     LPPGVMQAMRQAAGSLSGVVVLLGAPAKPRHHELTLSPLRDGRGRPTGQLLALHDVEE
                     RHALLLAEQAARRAAEEAQARLAFLAEASRVLGSSLDYEATLQRVVRLAVPRLAEYCA
                     VDLIHDD"
     gene            1342..1860
                     /locus_tag="VFU72_06165"
     CDS             1342..1860
                     /locus_tag="VFU72_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEU5423107.1"
                     /translation="MRNLELKVPVPALAEAREAMGRIGAVSMGSQAQTDTYYRVPHGR
                     LKLRQIAGQGGATLIAYDRPDEPASRYSDYLLAPVADPDALRATLALALGVLVVVRKR
                     RELWRYGATRIHLDAVDGLGSFVELETVFGGQPAAAAVAEHLLVRDRLGLAALVAIPG
                     GYADLLLAARER"
     gene            1930..2208
                     /locus_tag="VFU72_06170"
     CDS             1930..2208
                     /locus_tag="VFU72_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423108.1"
                     /translation="MAEQQTKQRVQGEGKSGTHSHPPMTHTHDHWHVSHHHTGDGKFE
                     HHASWHTHEHNHAELTHSHDYTQDEEQQHHSSEAHIHDHSAPATPRQG"
     gene            complement(2389..3162)
                     /locus_tag="VFU72_06175"
     CDS             complement(2389..3162)
                     /locus_tag="VFU72_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423109.1"
                     /translation="MSPDPPARGGQPFDPRPHLRRVAGQREGLLDVRVRLLWLRAEHP
                     EARVATEHVLLDERHAVFRATAELPSGARASGYGAASAAESDDYIERAETRALGRALA
                     VLGFGMQYTADFDLLEPEATVPEPAPAPVRPPRGEGEQPASPAAAEPASEERPAARRA
                     AEPPRIRMVPPPEESTPAPMPEPRPVPAAVPEAAQTEAGPFDMADYSWNDFWRWARPL
                     GYRSRTELDELLGVVTEEMTPREVRLLLLQYRAEHDQGE"
     gene            complement(3159..4103)
                     /locus_tag="VFU72_06180"
     CDS             complement(3159..4103)
                     /locus_tag="VFU72_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5423110.1"
                     /translation="MANAVQLEPGLFLIDLNFQGVPGVIGSYLLAGDGELALIETGPT
                     STIDALLAGVCAAGFAPEDISQLLVTHIHLDHAGAAGALLQRLPRARLLVHPQGAPHM
                     IDPTRLLASAARIFGENLLPLWGEFVPAPADRVEELPDEARVRVAGRELVALHTPGHA
                     YHHIAYWQPENGTLFTGDVAGVRLGGAPYVRPPTVPPELDLELWQESVTRMRGLAAKR
                     LYLTHFGGYDDIAWHLDDLLARLFFWAGWAGARLQAEPDTPTLTRELGELGDAEIVAK
                     TGRQELVMPYEIATAYQMTIDGMARYFRKRWAPRGGAR"
     gene            complement(4139..4933)
                     /locus_tag="VFU72_06185"
     CDS             complement(4139..4933)
                     /locus_tag="VFU72_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5423111.1"
                     /translation="MVELTFLGTGTSNGIPIIGCHCPVCTSPDPRDRRGRTSAVVRFD
                     GHTILIDTATELRLQAIQAGLDQVDAVLYTHAHADHVAGLDDLRRFNELNQAWLPVYA
                     DPLTAANLRERFAYAFQPQFPFFGGKPDLTLHEFTGPFTLFEREIIPIPVPHGRWQVQ
                     GFRFGPLVYVTDAKTIPPTSLELMHDADILVLNALRERPHPTHLSLGEALAIIEEIQP
                     RQAYLVHLCHELAHEVASARLPANVAVAYDGLRVCTGRAWEPPVLP"
     gene            5102..7435
                     /locus_tag="VFU72_06190"
     CDS             5102..7435
                     /locus_tag="VFU72_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5423112.1"
                     /translation="MTGQLRRSGWMDAITGHARASATEVEDREIVQPELFALLQRMTA
                     RLRSSAPLGELLSGLAASICGALETEIGLVRLLDRDHERLSVQGSHGVPPHLLGGLLG
                     ERSRALAEFLTLRPGSLITYTPDRPLVLQHATTIDTAEVQELQRLGAQHILILPLHHR
                     GELVGRLDLLRLRDVPFSAVEGALATTLGTLVAGAIFGPAHPDPGERYDRIIEASFAF
                     QQSIAPLANVGEMLQSIVEAAGRVVPCDRCYGLLWDGTRASFAPVAVAGADTTILAQL
                     KRLSFSFTPRDIPALAEVLGTDRPLVATAGQDRRLPDWLVEALGMHRLALVPLRSRRE
                     TLIGVLMLDMVDPEDDFSQRDLAVLSVIAPHAAMLVEHALLYEEVKRSSDRLALVNDI
                     GIELASLTDLEHLFRQIRLHVASVMTTSRFCLGLVLPDGESIEYRYALEDDLGQEPIT
                     RPLGGDPMSRAIRARQPILIETRESGDLSDWFPLEQEPRSESMLAVPVQVGHRAIGVI
                     AAFSEICNAYTQNDLDLLATVGLQAGVAIENARLYTLVQARGERRAYLLDQMLSRHEA
                     ERKTIAADIHNDTLQTLASSLYSLDLISRRAVEQNPQATQEELREVRDNLAENIDRLR
                     QITFQIRPSTLDILGLEPALREHARYIERSDNISISIEVDLFERLSNDVETAVYRIVQ
                     EAIDEVRGRAGVSYIVIRIKQVGELIIATLADNGESVSLDTVDEGTADLRPLSPAVRR
                     LVALRERIELAGGHLHTANLPGGGTTLQLVLPNRGAA"
     gene            7432..8370
                     /locus_tag="VFU72_06195"
     CDS             7432..8370
                     /locus_tag="VFU72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5423113.1"
                     /translation="MSALPETATVPLALVVEAQRRLLAAETLPELAEAVWALACRVSA
                     RPGVRLYRRTDDDLVLVQAHPDTVGEPAPPAECSAGLMVAWVPPAEAPAGAVVLEHDG
                     GDAAALRALAELAAQAFCLWLRNQEERERERRQFARYLHDGIVQDLAYLALQLDLLAR
                     GLPAESAETRRLAGRLRGEIDAAIGGLRGTIGDLRRARTPTVSGTTEAHAPDLERALL
                     AIVREALQNVRKHAHASRVAIELVGDQHELRLSVSDNGVGIAQREDAIAGEHFGLEQM
                     RELAGELGGELALEPAPGGGTRLAVHLPLHGSEWVW"
     gene            8383..9054
                     /locus_tag="VFU72_06200"
     CDS             8383..9054
                     /locus_tag="VFU72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423114.1"
                     /translation="MSDDAIRIVVADDHDLFREGLRQLLETIPAVEIVGEAADGQEAI
                     KLVAELHPDVVLMDINMPRVDGLRATEYIVKHFPTTNVVVLTMYQDDEYAIHAIRAGA
                     KSYLLKNSRSDEVIKAIQVASDGGSTIDATLAPALMREYHRLLTKTPSSNQRHLSERE
                     AMLLRLLASGFNNRQIADHLDLAESTVKNNLSALFQKIGVRDRTQAALYAIAQGYAAK
                     ASEPV"
     gene            9231..10415
                     /locus_tag="VFU72_06205"
     CDS             9231..10415
                     /locus_tag="VFU72_06205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5423115.1"
                     /translation="MQALSGVRVVDLTRVMTGPYATMLLGDMGADVVKIEQPGKGDDT
                     RAWGPPFIGDQSAYYLSVNRNKRGITLNLKHEAAREALWKLIDQADVVVENFSPGTAK
                     RLGFGYEAVRARRPNIVYCSISGFGQQGPEHDRVAYDQIIQGMSGLMSVTGFEDGPPT
                     RYGVPIADIAAGMFAAYAIVSALFSRERTGQGQYIDTSMLGGMVALLTYQAGIYFGSG
                     DAPGRTGNAHPIVAPYQTFRSQDGYVNVAAGNEGIYRRFCAAMGLERLLDDPRFQDNV
                     GRITNRAALAGEIEDTLQRFTTAEIVARLDQADVPCGPIYTVPEVFANPQAEYYQLRR
                     RVPHPTLGEVDQTGFPYRLSETPCEIRLAPPLLGQHTAEVLGELGYDQARIAAMRDGG
                     AI"
     gene            complement(10638..10952)
                     /locus_tag="VFU72_06210"
     CDS             complement(10638..10952)
                     /locus_tag="VFU72_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEU5423116.1"
                     /translation="MQNLRGLAWLLPTTSLLFVGYIVFAFLGALSFGLPIWLILLPSL
                     PALGLTAIALVVALLDVTKRPAAEISEESRIVWVLVLIIFPVVGLLPYWLVVMRRPSA
                     SA"
BASE COUNT         1767 a         3847 c         3995 g         1541 t
ORIGIN      
        1 tcatcgtcgt ggatcaagtc gaccgcgcaa tattcggcca ggcgcggcac ggccaggcgc
       61 acgacgcgct gcagcgtcgc ctcatagtcg agcgacgaac cgagcacgcg gctggcctcg
      121 gccaggaagg ccaggcgtgc ctgcgcctcc tccgccgcgc gccgggcggc ctgctcggcg
      181 agcaggagcg cgtgacgctc ttcgacatcg tgcagcgcca gcagttggcc cgtcgggcgc
      241 ccgcgcccgt cgcgcagcgg cgacagcgtc agctcgtggt gccgtggctt agccggggcg
      301 cccagcagca cgacgacacc cgacaggctt ccggccgcct gccgcatggc ctgcatcacg
      361 ccgggcggca gccgctccgc cagcggttgg ccgagggcat cgctggacca gccgagcatg
      421 cgctgcgccg ccgggttgac atcgaccaca cgggccgtgc gatcgaccac cacaatacca
      481 tcgctcatcc cctgcacact gagatcgcgc gccaccggca cgacgtcgag caggtgccag
      541 cgggcgaggt tccaggccag cagcagctcg acgatcggga aggcgaaggg tgtgaggtcc
      601 ggccaggacg ccggcgtgag cggcatcacg gtgaggagat tggccagcca gggcaccagg
      661 atcacgatca aaagggcggc ggcctggcgc cggaagagac gcagcgagtg gttgagaacg
      721 cgcagcagcg gcaggctggc gagcgctagc agcgcgtagg aatacgccaa gtgcgcccag
      781 aaccagaggc ccgggctgaa gatggaaaag tgctcggcgc ccgactgctc gacatcagcc
      841 cagtagaggt ggtgtgaccg gttcgtccag agcaggacca ccgtcaccag cggctcgatc
      901 gccagcagca ggagcgcccg ccgcgtgagc cagccggcgc gacgggtgta ggcgaaggcg
      961 aagatcagcc aggcgaacgg gatccaagcc gcgccggagt acgccagctt ggcccagaag
     1021 agcttggcgc ccatccccgc gctgaggtag gcggcgccat aggcggccgc ccataggctg
     1081 ccgctggcta cgagcagcag gagcgcgccc gagccgggag cgtcgcgccg ccgctgtacc
     1141 acggcgccaa cgaccaggcc gagggcggca gccaggaaaa gcgggcccgc gtagggcatt
     1201 gcctgccagt ccactgggaa ccctccgagg ccaagcatca cggagcatga ccatcatagc
     1261 ggccgcggat cacgctgaca aggcgaaacg acagcgggtc gcggcaagat gcgaagatgc
     1321 gggtgtggag aggaggaagc gttgcgcaac ctggaactga aggtgccagt gcccgcgctg
     1381 gccgaggcgc gggaggccat ggggcggatc ggcgcggttt ccatgggtag ccaggcgcag
     1441 acggacacct attaccgtgt cccgcacggt cgcctgaagc tgcgtcagat cgccggtcag
     1501 ggcggggcga ccctgatcgc ctacgaccgg cccgacgagc ccgccagccg ctactccgac
     1561 tacctgctgg cgccggtggc ggatccggac gcgctcaggg cgacactcgc gctggcgctc
     1621 ggcgtgctgg tcgtggtgcg caagcggcgc gagctctggc gctacggcgc cacacgcatc
     1681 catctggatg cggtcgacgg gctcggcagc ttcgtggagc tggagacggt gttcggcggc
     1741 cagcccgccg ccgcggccgt ggccgagcac ctcctcgtcc gcgaccggct cgggttggcc
     1801 gctctggtcg cgatccccgg cggttacgct gacctgctgc tcgccgctcg cgagcggtga
     1861 tgggtgttgg cacggcacat gcacgctggc gcaggtatcg tccgtcgtaa cgcgaaaggg
     1921 gagacgcgca tggccgagca gcagacgaag cagcgggtgc agggcgaggg gaaatcgggc
     1981 acgcacagcc acccgccgat gacgcacacc cacgatcact ggcacgtgag ccatcaccac
     2041 accggcgacg gcaaatttga gcatcacgcg tcgtggcaca cgcacgagca caaccacgcc
     2101 gagctgacgc acagccacga ctacacccag gacgaagagc agcagcatca ttccagcgag
     2161 gcgcacatcc acgaccactc ggccccggcc accccgcgcc agggctgagc gcgagctcgg
     2221 cgttggatgt tctcgctgta ggggcagacc tccgtgtctg ccctgtctca cgtgtgcctc
     2281 ggcaacgaga tcagggcgag cgtcgggggc agtcgtgaag acaattgcac gtgctgcgag
     2341 ggcaagggca gacacggcgg tctgccccta ccggaaacgg aagcgtccct actctccctg
     2401 gtcgtgctcg gcgcgatatt gcagcaggag gaggcgcacc tcgcgcgggg tcatctcttc
     2461 ggtgaccacg cccagcagct cgtccagctc ggtgcggctg cggtagccga gcgggcgcgc
     2521 ccagcgccag aagtcgttcc agctgtagtc ggccatgtcg aagggcccgg cctcggtctg
     2581 agccgcctct ggcaccgcgg cgggaacggg tcgcggctcc ggcatggggg cgggcgtcga
     2641 ctcctccggc ggcggcacca tgcgaatgcg gggcggctcg gccgcgcgtc gtgcggccgg
     2701 gcgttcctcg ctggccggct cagcggctgc cggcgatgcc ggctgctcgc cctcgccgcg
     2761 cggcggtcga accggcgccg gagccggctc gggcacggtg gcttctggct ccagcaggtc
     2821 gaagtcggcg gtgtactgca tgccgaagcc gagcacggcc agcgcgcggc cgagcgcccg
     2881 cgtctcggcg cgctcgatgt agtcgtcgga ctcggcggcg gaggcggcgc cgtagccgct
     2941 ggcgcgcgcg ccactgggga gttccgccgt ggcacggaag acggcgtgcc gctcgtcgag
     3001 cagcacgtgc tcggttgcaa cgcgcgcctc ggggtgctcg gcgcgcagcc agagcagccg
     3061 cacgcgcacg tcgagcagac cctcgcgctg cccggcaacc cggcgcaggt ggggccgggg
     3121 gtcgaacggc tggccgccgc gggccggagg atccgggctc atcgggcacc gccgcgcggc
     3181 gcccagcgct tgcggaagta gcgcgccatg ccgtcgatcg tcatctggta ggcggtggcg
     3241 atctcgtagg gcatcaccag ctcctggcgg ccggtcttgg ccacgatctc ggcgtcgccc
     3301 agctcgccca gctcacgtgt cagcgtcgga gtatccggct cagcctgcaa gcgcgcgccc
     3361 gcccagccgg cccagaagaa gaggcgggcc agcaggtcgt ccaggtgcca ggcgatgtcg
     3421 tcgtagccgc cgaagtgcgt cagatagagc cgcttggccg cgaggccgcg catgcgcgtc
     3481 acgctctcct gccacagctc cagatccagc tcgggcggca ccgtcggtgg gcgcacgtag
     3541 ggcgcgccgc ccagccgcac gccggccacg tcgccggtga agagcgtgcc gttctcgggc
     3601 tgccagtagg cgatgtgatg gtaggcgtgg ccgggcgtgt gcagggccac cagctcgcgc
     3661 ccggccacgc gcacccgcgc ctcgtcgggc agctcctcga cacggtcggc gggcgccggc
     3721 acgaattcgc cccagagcgg cagcaggttc tcgccgaaga tgcgcgccgc gctggccagc
     3781 aggcgggttg gatcgatcat gtggggcgcg ccctgcggat ggaccagcag gcgtgcgcgg
     3841 ggcaggcgct gcagcagcgc gcccgccgcg ccggcgtgat cgaggtggat gtgcgtcacc
     3901 aggagctggg agatgtcctc cggcgcgaag ccggccgcgc acaccccggc caacagggca
     3961 tcgatggtcg aggtcggccc ggtctcgatc agcgccagct cgccgtcgcc ggccaggagg
     4021 tacgagccga tgacaccggg cacaccctgg aagttcagat cgatcagaaa gagaccgggt
     4081 tcgagctgga ctgcgttcgc catcgcgcct ccttgtcagc cgcctccggc cggtcgaact
     4141 atggtagcac aggcggttcc cacgcccgcc cggtgcagac gcgcaggccg tcgtaggcca
     4201 ccgccacatt ggccggcagc cgcgcgctgg cgacctcgtg cgccagctcg tggcagagat
     4261 gcaccagata ggcctggcgc ggctggatct cctcgataat cgccagcgcc tcgcccaggc
     4321 tcaggtgcgt tgggtggggg cgctcgcgca gcgcgttgag caccaggatg tcggcgtcgt
     4381 gcatcagctc cagcgacgtc ggtgggatcg tcttggcgtc ggtcacgtag accagcgggc
     4441 cgaagcgaaa gccctgcacc tgccagcgcc cgtgcggcac cgggatcggg atgatctcgc
     4501 gctcgaagag cgtaaacggt cccgtgaact cgtgcagcgt cagatcgggc ttgccgccaa
     4561 agaaggggaa ctgcggctgg aaggcgtagg cgaagcgctc gcgcaggttg gcggcggtca
     4621 gcggatcggc gtagaccggc agccaggctt ggttcagctc gttgaagcgg cgcaggtcgt
     4681 ccagcccggc gacgtggtcg gcgtgggcgt gggtgtagag gacggcgtcg acctggtcga
     4741 gcccggcctg gatcgcctgc aagcgcagct cagtggcggt gtcgatcagg atcgtgtggc
     4801 cgtcgaagcg gaccaccgcc gaggtgcggc cacggcggtc gcgcggatcg ggcgaggtac
     4861 agacggggca gtggcagccg atgatcggga tgccgttgga ggttcccgtg cccagaaacg
     4921 tcagctcaac catcgcccgc gccgcgcctc ccccggcaag tataccgatc gtcctagcgc
     4981 gcccgtgaag acggtacttc ttggttgctt atccctgggt acccgccgcc cggcgttcgc
     5041 tatcatgagg ctaccaccgt cgcacggtgc ggtgtcgttc ggaaaccaga gcaggatgac
     5101 aatgaccggg caactgcgac gctccggctg gatggacgcg atcacgggtc acgcccgcgc
     5161 gagcgcgacc gaggtggagg accgcgagat cgtgcagccg gagctgtttg cgcttcttca
     5221 gcggatgacc gcccgcctgc gctcctcggc gccgctcggc gagctgctca gcggcctggc
     5281 cgcatccatc tgcggcgcgc tcgagaccga gatcgggctg gtgcgcctgc tcgaccgcga
     5341 ccacgagcgc ctgtcggtgc agggcagcca cggcgttccg ccgcacctgc tcggcgggct
     5401 cctgggcgag cgcagccgcg cgctggccga gttcctgacg ctgcggccgg gctcgctcat
     5461 cacctacacc ccggaccggc cgctggtact gcagcacgcc accaccatcg acaccgccga
     5521 ggtgcaggag ctgcagcggc tgggcgcgca gcacatcctg atcttgccgc tgcatcatcg
     5581 gggtgagctg gtcggccggc tcgacctgtt acggctgcgc gacgtgccgt tctcggcggt
     5641 cgaaggagcg ctggcgacca cgctggggac gctggtggcc ggcgcgatct tcggcccggc
     5701 gcaccccgac cccggcgagc gctacgaccg catcatcgag gcgagcttcg ccttccagca
     5761 gtcgatcgcg ccgctggcca acgtcggaga gatgctgcag agcatcgtcg aggcggccgg
     5821 acgggtcgtc ccctgcgacc gctgctatgg gctcctctgg gacggcaccc gggccagctt
     5881 cgccccggtg gccgtcgccg gcgccgatac gacgatcctg gcgcagctca agcgcctgtc
     5941 gttctccttc accccgcgcg acatcccggc gctggccgag gtactcggca ccgatcgccc
     6001 gctggtagcc accgccggcc aggaccggcg cctgcccgat tggctcgtcg aggcgctggg
     6061 gatgcaccgg ctggcgctgg tgccgctgcg cagccggcgc gagacgctga tcggcgtgct
     6121 gatgctcgac atggtcgacc cggaggacga tttcagccag cgcgacctgg cggtgctgag
     6181 cgtgatcgcg ccgcacgccg ccatgctggt cgagcacgcg ctcctctatg aggaggtcaa
     6241 gcgcagctcc gaccggctgg ccctggtcaa cgacatcggc atcgagctgg cctcgctgac
     6301 cgacctcgaa cacctcttcc ggcagatccg gctgcacgtc gcctcggtca tgaccaccag
     6361 ccgcttctgc ctcggcctgg tcctgcccga cggcgagtcg atcgagtacc gctacgcgct
     6421 ggaggacgac ctggggcagg agcccatcac acgcccgctg ggcggcgacc cgatgagccg
     6481 cgccatccgc gcgcgccagc cgatcctgat cgagacgcgt gagtcgggcg atctcagcga
     6541 ctggttcccg ctggagcagg agccgcgctc ggagtcgatg ctggcggtgc cggtgcaggt
     6601 ggggcaccgc gcgatcggcg tgatcgcggc cttcagcgag atctgcaacg cctacacgca
     6661 gaacgacctc gatctgctgg cgacggtcgg gctgcaggcc ggggtggcga tcgagaacgc
     6721 ccgcctctac acgctggtgc aggcgcgggg cgagcgccgg gcctacctgc tcgaccagat
     6781 gctgagccgg cacgaggccg agcgcaagac gatcgcggcc gatatccaca acgacacgct
     6841 gcagacgctg gccagcagcc tctacagcct cgatctgatt agccggcgcg ccgtggagca
     6901 gaatccgcag gcgacccagg aggagctgcg ggaggtgcgc gacaacctgg ccgagaatat
     6961 cgaccggctg cgccagatca ccttccagat ccggccgtcg acgctcgaca tcctggggct
     7021 ggagccggcg ttgcgcgagc acgcgcgcta catcgaacgc agcgacaata tcagcatcag
     7081 tatcgaggtc gatctgttcg aacggctgag caatgacgtg gagaccgccg tctaccgcat
     7141 cgtccaggag gcaatcgacg aggtgcgtgg gcgcgccggc gtcagctaca tcgtgatccg
     7201 gatcaagcag gttggcgagc tgatcatcgc cactttggcc gacaacggcg agtcggtctc
     7261 gctcgacacc gtcgacgaag ggacggccga tctgcggccg ctctccccgg cggtgcggcg
     7321 cctggtggcg ctgcgcgagc gcatcgaact ggcgggcggt cacctgcaca cggccaacct
     7381 ccccggcggc ggcaccacgc tgcagctggt cttgccgaat cgaggcgcag cgtgagcgcg
     7441 ctgcccgaga cggccacggt gccactggcg ctggtcgtgg aagcgcaacg tcgcctgctg
     7501 gcggccgaga ccttgccgga gttggccgag gccgtctggg cgctggcctg ccgggtgtcc
     7561 gcgcgccccg gcgtgcgcct ctaccggcgc actgacgacg acctggtgct ggtgcaggcg
     7621 cacccggata cagtgggcga gccagcgccg ccggccgaat gcagtgccgg gctgatggtc
     7681 gcctgggtgc cgccagccga ggcgccggcg ggcgcggtgg tgctggagca cgacggcggc
     7741 gacgcggccg cgctgcgggc gctggccgag ctggccgccc aggcgttctg cctgtggctg
     7801 cgcaaccagg aggagcgcga gcgagagcgg cgccagttcg cgcgctacct gcacgacggg
     7861 atcgtgcagg acctggccta cctggcgctc cagctcgatc tgctggcgcg cgggctgccg
     7921 gcggagtcgg cggagacgcg tcgcctggcc ggccgcttgc gcggcgagat cgacgcggcg
     7981 atcggcggcc tgcgcggcac gatcggcgat ctgcgccgcg cgcggacacc gaccgtgtcc
     8041 gggacgacgg aggcgcacgc gccggatctc gaacgcgcgc tgctggcgat cgtgcgggag
     8101 gcgctccaga acgtgcgcaa gcacgcgcac gcgtcgcgtg tagcgatcga gttggtggga
     8161 gatcagcatg agcttcgcct cagcgtcagc gacaacgggg tcgggatcgc gcagcgcgaa
     8221 gacgccatcg cgggcgagca tttcggtttg gagcagatgc gcgagctggc cggtgaactg
     8281 ggcggcgagc tggcgctcga gccggccccc ggcggcggta cgcgcctcgc ggttcatctt
     8341 ccgctacatg ggagtgagtg ggtttggtag gagggtgtca gggtgagtga cgacgcgatt
     8401 cgcatcgtgg tagccgacga ccacgacctc tttcgcgagg gtctccggca attgctggag
     8461 acgatccccg cggtcgagat cgtgggcgag gcggccgacg ggcaggaggc gatcaagctc
     8521 gtcgccgagc tgcacccgga tgtggtcttg atggacatca acatgccgcg ggtcgatggg
     8581 ctgcgcgcca ccgagtacat cgtcaagcac ttcccgacga cgaatgtggt cgtgctgacg
     8641 atgtatcagg acgacgagta cgccatccac gccattcggg ccggcgccaa gagctatctg
     8701 ctcaagaaca gccgctccga cgaggtgatc aaggccatcc aggtggcctc cgacggcggc
     8761 tcgacgatcg acgcgacgct ggcgccggcg ctgatgcgcg agtatcaccg gctgttgacc
     8821 aagacgccca gttccaacca gcgccacctg agcgagcgcg aggcgatgct gctgcggctg
     8881 ctggccagcg gcttcaacaa ccgccagatc gccgaccatc tcgacctggc cgagagcacg
     8941 gtcaagaaca acctctcggc gctcttccag aagatcggcg tgcgcgaccg cacccaggcc
     9001 gccctctacg ccatcgccca gggctacgcc gccaaggcca gcgagccggt ctgacccagc
     9061 ttctcctccc tctgtctccc cgccgagcgg tcgtgtagac tcgcctcggc gggcgcgacg
     9121 ttttccccca gggccagcgc gtcccaagcc cggcgcggag cgctccgggt taggtggcgc
     9181 tgcccggcgg gctttgcgcg gaggatggac aggcgagagg agcggggctg atgcaggcgc
     9241 tgagcggggt gcgggtggtc gatctgacgc gggtcatgac cgggccgtat gccacgatgt
     9301 tgctcggtga catgggcgcc gacgtggtca agatcgagca gccgggcaag ggtgatgaca
     9361 cccgcgcctg ggggccgccc ttcatcggcg accagagcgc ctactacctg agcgtcaacc
     9421 gcaacaagcg cggcatcacc ctcaacctca agcacgaggc cgcgcgcgag gcgctctgga
     9481 agctgatcga ccaggccgac gtggtggtcg agaacttcag ccccggcacc gccaaacggc
     9541 tcggcttcgg ctacgaggcc gtgcgcgcgc ggcgcccgaa catcgtctat tgctcgatct
     9601 ccggcttcgg ccagcagggg ccggagcacg accgcgtggc ctacgatcag atcattcagg
     9661 gcatgagcgg gctgatgagc gtcaccggct tcgaggacgg gccgccgacg cgctacggcg
     9721 tgccgatcgc cgacatcgcc gccggcatgt tcgccgccta cgccatcgtc agcgccctct
     9781 tcagccgcga gcgcactggc caggggcagt acatcgacac ctcgatgctc ggcggcatgg
     9841 tcgcgctctt gacctatcag gccggcatct acttcggcag cggtgacgcg cccggccgca
     9901 ccggcaacgc ccacccgatc gtggcgccct accagacctt ccgctcccag gatggctacg
     9961 tcaacgtggc cgccggcaac gagggcatct accgccgctt ctgcgcagcc atgggcctgg
    10021 agcggctact cgacgacccg cgcttccagg acaacgtggg gcgcatcacc aaccgcgccg
    10081 cgctggccgg cgagatcgag gacacgctgc agcgcttcac caccgccgag atcgtggccc
    10141 ggctcgacca ggccgacgtg ccctgcggcc cgatctacac cgtgcccgag gtcttcgcca
    10201 acccgcaggc cgagtactac cagttgcgcc ggcgcgtgcc gcacccgacg ctgggcgagg
    10261 tcgaccagac cggcttcccc taccgcctca gcgagacgcc ctgcgagatc cgcctggcgc
    10321 cgccgctgct gggccagcac acggccgagg tgctgggcga gctgggctac gaccaggcgc
    10381 ggatcgccgc catgcgcgac ggcggcgcga tctagagcgc tatgcgcgat ccgaggcggc
    10441 cgggcgccat cccctgtagg ggcagacctc tgtgtctacc ctcgccgccc tggcaattgc
    10501 cctgaccaac gccgctcacg ttgccctatt cccccgttac cgcgccagcg gggcgacagg
    10561 gcagacacag aggtctgccc ccaacaggcg tcaccgcgtg cgcgaatctc gcagggtttg
    10621 gttcagggga gcaacgatta cgcggaggcg gacgggcgac gcatgaccac cagccagtag
    10681 gggagcagcc cgaccaccgg gaagatgatc agcaccagca cccagacgat gcggctctcc
    10741 tcgctgatct cggcggcggg acgcttggtg acgtccagca gcgccaccac cagcgcgatg
    10801 gcggtcaggc cgagcgccgg caggctgggc agcaggatca gccagatcgg caggccgaag
    10861 ctgagcgcgc ccaggaaggc gaagacgatg tagccgacga agagcaggga ggtggtgggg
    10921 aggagccagg ccagacccct caggttctgc atcgtatctc caagactgcc gaacagcaga
    10981 gtgggcactc cgtgaccgct ggtcgctggg agtgcccacg aggaaccatc gatcgggaag
    11041 cgtggcgcat cgcgctacct ccctactccg gccttggctt ccttgcggcg ccgcttgtgc
    11101 tccgccttgc gcgccttggc ccggcgctgc tcgctcttgc tgacgaagcg
//
LOCUS       DASUBI010000323         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_732708, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000323 DASUBI010000000
VERSION     DASUBI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732708"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFU72_06215"
     CDS             complement(<1..560)
                     /locus_tag="VFU72_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5423117.1"
                     /translation="MTSPALRDSLRRLPAISTILADPVILPLLHQIQPELATKIAQRV
                     VDDIRTDILAGAQGVSADDVIPAVARELQSLLAPKLHAVINGTGVIVHTNLGRAPVSL
                     AAAAAMAEAAARYTPLELELTSGRRGGRMAELGRLLGLLTGAEATLVVNNNAAAVLLV
                     LSALCAGRAVVLSRGQAVEIGGGFRVP"
     gene            complement(639..716)
                     /locus_tag="VFU72_06220"
     tRNA            complement(639..716)
                     /locus_tag="VFU72_06220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Pro,seq:cgg)
     gene            863..936
                     /locus_tag="VFU72_06225"
     tRNA            863..936
                     /locus_tag="VFU72_06225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Arg,seq:cct)
     gene            1018..1602
                     /gene="raiA"
                     /locus_tag="VFU72_06230"
     CDS             1018..1602
                     /gene="raiA"
                     /locus_tag="VFU72_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5423118.1"
                     /translation="MELQIKTRGVQLTPKLEEYVERRFQKLDRVSENVLEAKLELRTE
                     KNRTGGEQAVAQITIATKNAILRAEEKNRDLHTAIDLAAERMARQIRRFHDKKVFTRR
                     RQKAVGEPEAAVAAAADGLSGVPAEEPDELPLAALVRRKRFKIQPLSEDEAIEHMELL
                     GHDFFVFFNPDAAQINVLYRRKNGQYGLIQPELA"
     gene            complement(1672..2235)
                     /locus_tag="VFU72_06235"
     CDS             complement(1672..2235)
                     /locus_tag="VFU72_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423119.1"
                     /translation="MVAQIQNQPGVLSVVPGITPSDTLTIEVSNGNRHVSVQNNSGSK
                     IAELWKVGDQVWASAAGSPPVQASANTPVVGVLVPLALADQQLITTFTAQGATYTVSG
                     SEDINGVATQVETATYQINPSDNIFLGSGPYTVNSTLWVAKSGGYLVQAQMLLSQGAG
                     TPSASSPSVKINVTQVGKSITITPPSS"
     gene            2261..2689
                     /locus_tag="VFU72_06240"
     CDS             2261..2689
                     /locus_tag="VFU72_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423120.1"
                     /translation="MLKAVARLTGPLTCVPVAGEAEADEDVVGLAVAILLTGEAVAVA
                     TALGDAAPLVGLAAALVVGTALAAAAGEDTAAVVGLLEPPPQALRMSAPASESAATRA
                     KTGFIQVPPEDYVIRESSLGAFRAAGCAVQILCPYDRRLG"
     gene            2757..>3196
                     /locus_tag="VFU72_06245"
     CDS             2757..>3196
                     /locus_tag="VFU72_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423121.1"
                     /translation="MDVEQSNWVVRGALCSLIAVGLLAGVGIWARHAAVSPGQVPLLD
                     PHSRGFWALGLLAALVSAALFQALAPLPDTLVGTAGAFGAPAPATAPSAWVVPFIVSL
                     TSVMLLGVYHSPASIGVIVLGAFVVLAGAVLAQRALRTGDDETR"
BASE COUNT          515 a         1049 c         1127 g          505 t
ORIGIN      
        1 ggcacgcgga agccgccgcc gatctcgacc gcctgcccac gcgagaggac gaccgcgcgc
       61 ccggcgcaga gcgcgctgag caccagcagg acggcggcgg cgttgttgtt gacgaccagc
      121 gtcgcctcgg ctccggtcaa gagtcccagc aagcggccca actcggccat gcgcccgccg
      181 cgccggccgc tcgtcagctc cagctcaaga ggggtgtagc gcgcggcggc ctcggccatg
      241 gccgcggcgg cggccaggct gaccggcgcc cggcccaggt tggtgtggac gatcactccc
      301 gttccgttga tgaccgcgtg cagcttgggc gccagcagcg actgtagctc gcgagcgacc
      361 gccggaatga catcgtccgc cgagacgccc tgagcacccg ccaggatatc ggtgcgaata
      421 tcatcgacga cgcgctgcgc gatcttcgtc gccagttcgg gctgaatctg gtggaggagc
      481 ggcaggataa cgggatcggc gagaatggtc gagatggcgg ggagccggcg aagcgagtcg
      541 cgaagcgcgg gcgaggtcat ctggcgaccg tctcccttcg acgcccggcg ggtggggcgc
      601 gggacaaaca aagaaggagc ggcgatgccg ctccaacatg gtcggggaga gaggatttga
      661 acctccgacc tccgcgtccc gaacgcggcg cgctaaccgg gctgcgccac tccccgagtg
      721 cgctacagta tagcagatag ctgggcggcg cggcaaatgc gcccgcgccc atgcccatcg
      781 cccatgagcc ggcgtcaaga ttggaaaccg ggccggaagt taggctatac ttaggtgctt
      841 ttccgcatgt agctgcggat ttgcccgcgt agctcagggg aaagagccgg ggcgtcctaa
      901 gccctgtggc cgggggttcg agtccctccg cgggcgcaaa ctggccagtc atcgcccgat
      961 gacgccggtc cccaattgtc tctgccagga gcacgagtaa gagaggggaa cagcctgatg
     1021 gaactccaga tcaagacccg cggcgtccag ctcacgccca agctcgaaga atacgtcgag
     1081 cggcgcttcc agaagctcga ccgggtgagc gagaacgtgc tcgaggccaa gctagagctg
     1141 cgcaccgaga agaaccgcac gggcggcgaa caggcggtgg cccagatcac catcgcgacc
     1201 aagaacgcta tcttgcgggc cgaggagaag aaccgcgacc tgcacaccgc catcgacctg
     1261 gccgccgagc gcatggcgcg ccagatccgg cgcttccacg acaagaaggt cttcactcgc
     1321 cggcggcaga aggcggttgg tgagccggag gcagcggtcg cggcggcggc tgacggactc
     1381 tccggcgtgc cggcagagga acccgacgag ctgccgctgg cggcgctcgt ccggcgcaag
     1441 cgcttcaaga tccagccgct gagcgaggac gaagcgatcg agcacatgga gctgctcggc
     1501 cacgatttct tcgtcttctt caaccccgac gcggcccaga tcaacgtgct ctaccggcgc
     1561 aagaacggtc agtacggtct gatccagccc gaactggcct gatccatcgc ctgaacgcgg
     1621 gacgcccgga gcatgtgctc cgggcgtcgc gtcgtgggct gccggcccag tttacgagct
     1681 gggtggggtg atggtgatcg acttgcccac ctgggtcacg ttgatcttga cgctggggct
     1741 gctggcgctc ggcgtgccgg ctccctgcga gagcagcatc tgcgcctgca cgagataacc
     1801 gccgctcttg gcgacccaga gcgtcgagtt gaccgtgtag gggccgctgc ccaggaagat
     1861 gttgtcgctg gggttgatct gataggtggc cgtctcgacc tgcgtcgcga cgccattgat
     1921 atcttccgag ccggacacgg tgtaggtcgc gccctgcgcg gtaaacgtcg tgatcaattg
     1981 ctggtcggcc agcgccagcg gcaccagcac cccgacgacc ggcgtgttcg cgctcgcctg
     2041 caccggcggc gagcccgcgg ccgacgccca cacctgatcg ccgactttcc acagctcggc
     2101 gatcttgctg ccggagttgt tctgcaccga aacgtggcgg ttgccgttgg agacctcgat
     2161 cgtcaacgtg tcggacggtg tgatccccgg gacgaccgag agcacgcccg gctggttctg
     2221 gatctgggcg accatctggt acgagccctg gctttcgagg ttgctgaaag cggttgccag
     2281 attaaccggc ccgctgactt gcgtccctgt cgccggcgaa gcggaggccg acgaggatgt
     2341 cgtcgggctg gcggtggcta tactgctcac gggggaagcg gtggccgtcg ccacggcgct
     2401 cggcgacgcg gcgccgctgg tggggctcgc ggccgcgctg gtggttggga cggcgctggc
     2461 cgcggcggcc ggcgaagaca ccgccgcggt ggtggggcta ctcgaaccgc cgccgcaggc
     2521 gctaaggatg agcgctcctg ccagcgagag cgcggcaact agggcgaaga ccggtttcat
     2581 tcaagtacct cctgaggact acgtgatacg ggaatcatcg ctcggcgcct tccgcgccgc
     2641 cggctgcgcg gtgcaaatcc tatgcccgta cgatcgccgg cttggataat cgcctgtggg
     2701 tacgttagga ttgaccgccg agggccgacc atctcgatgc tgaaccgtgg ggagcaatgg
     2761 acgtcgagca gtcgaattgg gtcgtccgcg gagcgctctg cagcctgatc gccgtcggcc
     2821 tcctggccgg cgtcggcatc tgggccaggc acgccgccgt ctcgcccggc caggtgccgc
     2881 tgcttgaccc gcacagccgc ggcttctggg cgctcgggct gctggcggca ctggtaagcg
     2941 ccgcgctctt tcaggccctg gcccccttgc cggatacgct cgtcggaacc gccggcgcgt
     3001 tcggtgcccc cgcgccggcg acggcgccga gtgcctgggt ggtgccgttc atcgtcagtc
     3061 tcacttcggt gatgttgctc ggcgtctatc actcgccggc gtcgatcggc gtgatcgtgc
     3121 tgggcgcctt cgtggtgctg gcgggcgccg tcctggcgca gcgagcgctg cgcaccggcg
     3181 acgacgagac acggca
//
LOCUS       DASUBI010000324         6063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1310739, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000324 DASUBI010000000
VERSION     DASUBI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310739"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFU72_06250"
     CDS             complement(<1..322)
                     /locus_tag="VFU72_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5423122.1"
                     /translation="MRTTLVETVPRERRQDLLTYDGGGDLPFAVETRGLSKRYGARLA
                     VDRLDLRVRRGEVYGFLGPNGAGKTTTLRMLLGLVRPTSGTAVVLDAPPGASRGLARI
                     GALIE"
     gene            538..1584
                     /locus_tag="VFU72_06255"
     CDS             538..1584
                     /locus_tag="VFU72_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423123.1"
                     /translation="MGEAFSHPVPAAAGASSGVLSIQALDWAVAGLSVLVTGGFTLDV
                     WAHNHGKVDKSFFTPWHAILYTSVALTGLFLAYQASRRGRSLPTGYARSLAGVALFIV
                     GGVLDLGWHTLFGVEADIETLLSPTHLLLALAGLLILTGPLRSLSRRDAEGWRELGPA
                     MIAATLVLVVFGIFTQYAHPFTEIAAVEPPGRQALGISSILLQTALLMGMVLLLVQRW
                     ALPPGALTLLFAVNGLAMAIIQDQYHLLPVMVLGGLAGDGLLRWLHPPGPSPARLRMF
                     AAGVPMVLYALYFLDVFLTGTVTWSTHLWAGSIVLAGVVGLLLSMLVSSPLGAAAVDP
                     PACRSRTDPSTSSG"
     gene            complement(1711..2781)
                     /locus_tag="VFU72_06260"
     CDS             complement(1711..2781)
                     /locus_tag="VFU72_06260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5423124.1"
                     /translation="MLTAALLTLGDPGRLTGGYLFHQRLAELAPEFDARIAFVSFPER
                     RFPLAALDAPRVLRRARATGAQVLVLDSIAAAFLGPRFLLRPPELPLVGMLHQPPGGI
                     DYGPARATVQAWLDRLAERRAARLLAASESLAADLIASGVPPGRVRVVPPGRDVAPLP
                     AGPLPELRRGRAMALLCVGNWVPRKGIDSLLEAVARLPSEAATLHLAGDDRAEPRYAA
                     RLYWRLAAQDLAGRVVVHGPLPREQVAALYAAADAFALPSLKEPFGTVYGEAMAFGLP
                     VVGWRAGNLPYLAEHEREGLLAAPGDLAGLTGALARLAADSALRERLGAAAKARALSR
                     PTWRETAEHFFAALREVARQAG"
     gene            2780..3892
                     /locus_tag="VFU72_06265"
     CDS             2780..3892
                     /locus_tag="VFU72_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5423125.1"
                     /translation="MRVVQVVPTPFGRAGLFGGGERYPLELARALSQSGEVECRLITF
                     GPQLEEARDPSGLHVSVLPARGYLGGHPAHPIAPRLVGALAGADLIHTHHLRSLPSRL
                     AALAAWPRRQPAVVTDHGLQGGDWAGLLPRLFSRFLLVSRYSARLLDAPPERTRVIYG
                     GADPARFQPDPDARREGVLFVGRLTPHKGIDRLLAALPPGAHLRVAGSDGHDPLPPER
                     DYPRYLRQLAAGRDVDFLGPIADDDLPALYRTASVLVLPSVERTIFGKEIACSELLGL
                     VLLEAMASGTPVIASRLGGLAEVVEDGVTGFLVEPGAVAELRERISQLLGDLALARRL
                     GRNAREAVLARFTWDACARRCLDAYQELVDGSGRAR"
     gene            4120..4608
                     /locus_tag="VFU72_06270"
     CDS             4120..4608
                     /locus_tag="VFU72_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423126.1"
                     /translation="MSGVIYLVQNDDSERLLEMTDTAYDQEFVGRAGAVEVEVFKRIL
                     QWSEQRELSSWFGTGAQYGTCYPTYPTLMHKGECSWVFALLTYGSVEIEFGTMKTKPP
                     LSDDKRREIVRRLNEIPGARIPENRLSGYPTIPLSRIIDEAALRQFLSVFGWVVEEVR
                     RL"
     gene            complement(4848..>6063)
                     /locus_tag="VFU72_06275"
     CDS             complement(4848..>6063)
                     /locus_tag="VFU72_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5423127.1"
                     /translation="SATIDFADPVIKADPYPLYRELRETQPVFWNGQGWFVFRYDDVL
                     RLFNDPRISSARTGSLLSVLPPDVQRELEPMRTVLASRMLLTDAPTHTRLRNLVTKAF
                     SAKAANVKRERIQALCDQLLNGLLARDELELMEEFASPLPSYVIADMLGVSPERQPDF
                     ARWSRDQVRIYDRPGTLGERVAVMRQGQASMLETKAYLEEVIAARRRQPREDLITRLI
                     EAEEAGDRLTTDEMVVMIIALLVGGNNSTAHLIGNAVLTLLRHPEALARLRAEPRLIR
                     PAIEEVLRFECPVQTTSRVAKERIEIGGQVIEAGQNVFLVLASANRDERQFPDPDRLD
                     ITREPNRHLDFAHGPHFCLGSAIARAESQIAVNTLIQRCPELELAGGEIEWLDGFSFR
                     GPKRLPLRCGAG"
BASE COUNT          841 a         2134 c         2138 g          950 t
ORIGIN      
        1 cctcgatcag ggcgccgatg cgggccagcc cgcgcgacgc ccccggcggc gcgtcgagca
       61 cgacggccgt gcccgaggtg ggccggacga gccccagcag catgcgcagt gtggtcgtct
      121 tgcccgcgcc gttgggcccc aggaagccgt agacctcgcc ccggcgcacg cgcagatcga
      181 ggcggtcgac ggccagccgc gcgccgtagc gcttgctcag gccgcgcgtc tcgacggcga
      241 acgggaggtc gccgcctccg tcgtacgtca gcaggtcctg gcgccgctcc cgtggcaccg
      301 tctccaccag tgtcgtccgc atggcatcgc cttccgtgcc cgtctgaatc aaggccgccc
      361 ggcccatccg gacggcactt ttcaatgatc ggccgcgcga ggcacgaagc gatgcggcga
      421 acgctcacga aacaggcacg atgcgctccc agtacgggta acgccccgcc tgtgttaggc
      481 tcatgcctta ccgatgagga gtggcaggcg cgtcgcgcgt tcggaaggga taccgcgatg
      541 ggcgaggcat tctcacaccc ggtgccggcc gccgctggcg cttcctccgg ggtgctgagc
      601 attcaggccc tggactgggc cgtggccggg ttgtcggtgc tcgtcaccgg cggattcacg
      661 ctggacgtct gggcacacaa ccacggtaag gtcgacaaat ccttcttcac gccctggcac
      721 gcgatcctct acacgagcgt cgccctcacg ggtctcttcc tggcctacca ggcgagccgt
      781 cgcggtcgct cgctgccgac cggctacgcc cgctcgctgg ccggcgtggc actcttcatc
      841 gttggcggag tcctcgacct cggctggcac acgctcttcg gcgtagaggc cgatattgag
      901 acgctgctca gcccgacgca cctgctgctg gcgctggccg gcctcctgat cctgaccggg
      961 ccgctgcgct cgctcagccg gcgtgatgcg gagggctggc gggagctggg accggccatg
     1021 atcgcggcga cgctggtgct ggtcgtcttc ggcatcttca cgcagtacgc ccacccgttc
     1081 accgagatcg ccgccgtcga gccgccgggt cgccaggcgc tcggcatcag tagcatcctg
     1141 ctgcagacgg cgctgctgat ggggatggtg ctgctgctgg tgcagcgctg ggccctgcca
     1201 cccggcgccc tgacgctcct cttcgcggtg aacggcctgg cgatggcgat catccaggat
     1261 cagtaccacc tcctgcccgt gatggtgctc ggcgggctgg ccggggacgg gctgctgcgc
     1321 tggctgcacc cgcccggccc ctcccccgcg cgcctgcgca tgttcgccgc cggggtgccg
     1381 atggtgctct acgccctcta cttcctggat gtcttcctga ccggcacggt cacctggtcg
     1441 acgcacctct gggcgggctc gatcgtgctg gccggggtcg tcggactgct gctgagcatg
     1501 ctggtcagct ccccgctggg cgcggcggct gtcgacccgc ctgcttgccg ctccaggacg
     1561 gatccttcga caagctcagg atgacagagt agggatgagg cgaacgaccg ggattgtcct
     1621 catccggtag gccccgcggc aaccctgtca tcctgagctt gtcgaaggat ccgtcctcac
     1681 ccgatcccca atgtgttcgc cagccgcgcc ctagccggcc tgcctcgcca cctcgcgcag
     1741 cgcggcgaag aagtgttcgg cggtctcccg ccaggtaggg cggctgaggg cccgcgcctt
     1801 cgcggcggcg cccaggcgct cacggagcgc ggagtcggcg gccaggcgag ccagcgcacc
     1861 ggtcaacccg gccaggtcgc cgggcgcggc cagcaacccc tcgcgctcgt gctcggccag
     1921 ataggggagg ttgccggcgc gccagccgac caccggcagc ccgaaggcca tcgcctcgcc
     1981 gtagaccgtc ccgaacggct ccttcaaact gggcagggcg aaggcgtcgg cggcggcgta
     2041 gagcgcggcg acctgctcgc gcggcagcgg tccgtggacc accacgcgtc cggccaggtc
     2101 ctgcgcggcc agccgccagt agagccgcgc ggcgtagcgc ggctcggccc ggtcgtcgcc
     2161 ggccaggtgg agcgtggcgg cctcggacgg caagcgcgcc acggcttcca gcaggctgtc
     2221 gatccccttg cgcggcaccc agttgccgac gcagagcagc gccatggctc gcccccggcg
     2281 cagctcgggc agcggcccgg ctgggagagg ggccacgtcg cggccgggcg gcaccacgcg
     2341 cacgcgcccg ggcggcacgc cgctggcgat caaatcggcg gccagcgact cgctggcggc
     2401 cagcaggcgt gcggcgcggc gctcggccag ccggtcgagc caggcctgca ccgtggcgcg
     2461 cgccggacca tagtcgatcc cgcccggcgg ctggtgcagc atcccgacca ggggcagctc
     2521 gggcgggcgc agcaggaagc gcggcccgag gaaggcggcg gcgatgctgt cgagcacgag
     2581 cacctgggcg ccggtagcgc gcgcccggcg cagcacgcgc ggggcgtcga gcgcggccag
     2641 cgggaagcgg cgctcgggga aggagacgaa cgcgatgcgc gcgtcgaact ccggcgccag
     2701 ctcggccagc cgctggtgaa agagatagcc gccggtcagg cggccgggat caccgagcgt
     2761 gagtagggca gcggtcagca tgcgtgtcgt ccaggtcgtt cccaccccct tcggccgcgc
     2821 cgggctcttc ggcggcggcg agcgctaccc gctggagctg gcccgcgcgc tgtcccaaag
     2881 cggcgaggtc gaatgccgct tgatcacctt cggcccccag ctggaggaag cgcgcgaccc
     2941 cagcggtctg cacgtctccg tgctgccagc gcgcggctac ctgggcggcc acccggcgca
     3001 ccccatcgcc ccgcgcctcg ttggcgcgct ggccggcgct gatttgatcc acacccacca
     3061 tctgcgcagc ctgccgagcc ggctggcggc gctcgcggcc tggcctcgcc gccaaccggc
     3121 tgtcgtcacc gaccacggcc tgcaaggcgg cgactgggcc gggctgctgc cgcgcctctt
     3181 cagccgcttc ctgctcgtct cgcgctactc cgcccggctc ctcgacgcgc ctccggagcg
     3241 cacgcgcgtc atctacggcg gcgccgatcc ggcgcgcttc cagccggatc ccgacgcgcg
     3301 gcgcgagggc gtcctcttcg tcgggcgcct gacgccgcac aaggggatcg accgcctgct
     3361 ggcggcgctg ccgcccggcg cgcacttgcg cgtggccggc tcggacggcc acgatccttt
     3421 accgcccgag cgcgactatc cgcggtacct gcgccagctc gccgccgggc gcgacgtcga
     3481 cttcctcggc ccgatcgccg acgacgacct gccggcactc taccgcacgg cctccgtcct
     3541 cgtcctcccc tccgtcgagc gcaccatatt tggaaaagag attgcctgct cggagctgct
     3601 ggggttggtg ctgctggagg cgatggccag cggcacgccg gtcatcgcca gccggctcgg
     3661 cgggctggcc gaggtggtcg aagacggcgt gaccggcttc ctggtcgagc cgggcgctgt
     3721 ggccgagctg cgcgagcgca tctcgcaact gctcggtgac ctggcgctgg cccgccgcct
     3781 cggccgcaac gcccgcgagg ccgtcctggc ccgcttcacc tgggacgcct gcgcccggcg
     3841 ctgcctggat gcctaccagg agctggtcga cggaagcggg cgtgcgcggt gacgcgggga
     3901 aggaaagctg cctatcgagg acattgcgtg cagggtgagg acggatcctt gcgctgtcgt
     3961 cagccacctc ctcttgtcgc gggcggtgcg agagggccgt cgcctctgtc cgctcttccc
     4021 tgtccctgct acactgccgg tgacgacccc cattgcagta tctaccacgt cgcgcgcatg
     4081 ggcgtaccgt acgccgcacg gtagggagct gttggttcga tgagtggtgt gatctacctc
     4141 gtgcagaacg acgatagcga acgactgctg gagatgacgg acacggcgta cgaccaggag
     4201 ttcgtcgggc gtgccggcgc cgtcgaggtc gaggtcttca agcgcattct tcaatggagt
     4261 gaacagcgcg agttatcgtc gtggttcggg acgggagcgc aatacggcac ctgttatccc
     4321 acgtatccca cgctcatgca taaaggggag tgctcctggg tgtttgcgct gctgacgtat
     4381 ggatcggttg agatcgagtt tggcacgatg aagaccaagc ccccgttgtc agacgacaaa
     4441 cgccgcgaga tcgtgcgccg cctcaacgag atacccggtg cgcgcattcc cgagaatagg
     4501 ctgtccggct atcccaccat tccgctgtcg cggatcatcg acgaggctgc gcttcggcag
     4561 ttcctgagcg ttttcggctg ggtcgtcgag gaggtgcggc ggttgtaagg caggacggga
     4621 tggtaaaggt ttggcgcata tgtgtaggtt cggcgcccgt cggcgcgcgg ggctaaagcc
     4681 accgcgctaa ccttttacga aagcctgctc aagcaggctg gagtttgcgc aatgctcagc
     4741 ctgcttcagc aggcttcgcc tcctagcgcg gcggctttag ccccgcgcga tcgtgagcct
     4801 gggacggtcg aattcgtgag ccttcatgat ttccccgcgc ccacgcctca ccccgctcca
     4861 catcgcagcg gcaaccgttt cgggccgcgg aaggagaagc cgtcgagcca ctcgatctcg
     4921 ccgccggcta gctccagctc ggggcagcgc tggatgagcg tgttgaccgc gatctgggac
     4981 tcggcgcggg cgatggccga accgaggcag aagtgcgggc cgtgggcgaa gtcgagatgg
     5041 cggttcggct cgcgcgtgat gtcgaggcgg tcggggtccg ggaactggcg ctcgtcgcgg
     5101 ttggccgagg ccagcaccaa aaagacgttc tggcccgcct cgatcacctg cccgccgatc
     5161 tcgatgcgct ctttggccac ccggctggtc gtctgcaccg ggcactcgaa gcgcagcacc
     5221 tcctcgatgg ccgggcggat caggcgtggc tcggctcgca gccgtgccag cgcctcaggg
     5281 tgccgcagca gcgtcaaaac ggcgttgccg atcaggtggg cggtcgagtt gttgccgccg
     5341 accagcaggg cgatgatcat caccaccatc tcgtcggtcg tcagccggtc gcccgcctcc
     5401 tcggcctcga tcagccgcgt gatcaggtcc tcgcgcggct ggcgacggcg cgcggcgatg
     5461 acctcttcca gatacgcctt cgtctccagc atggacgcct gaccctggcg catcaccgcc
     5521 acgcgctcgc cgagggtgcc ggggcggtcg tagatgcgca cctgatcgcg gctccagcgg
     5581 gcgaagtcgg gctgccgctc gggcgagaca cccagcatgt cggcgatgac gtagcttggc
     5641 agcggggagg caaactcctc catcagctcc agctcgtcgc gcgctaagag gccgttcaac
     5701 aattggtcgc agagcgcctg gatgcgctcg cgcttgacgt tggccgcttt ggccgagaag
     5761 gccttggtga ctaggttccg cagccgggtg tgggtcggcg catcggtcag cagcatgcgg
     5821 ctggcgagga ccgtccgcat cggctccagc tcgcgctgca cgtcgggcgg taggacggag
     5881 aggagcgagc cggtgcgcgc cgaggagatg cgcgggtcgt tgaagaggcg gagcacgtcg
     5941 tcgtagcgga agacgaacca gccctgcccg ttccagaaga ccggctgcgt ctcgcgcagc
     6001 tcgcggtaga gcgggtaggg gtccgccttg atgaccgggt cggcgaagtc gatcgtcgcg
     6061 gac
//
LOCUS       DASUBI010000325         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_908160, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000325 DASUBI010000000
VERSION     DASUBI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908160"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFU72_06280"
     CDS             complement(<1..675)
                     /locus_tag="VFU72_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423128.1"
                     /translation="MTATPSASGGRSLPFPRLLAFSTGSTPVYIMAILVGVYLPKFYA
                     GHIGLTLLALGGVMATVRLTDLGFDLALGWLMDKTHTRFGRYRPWYAAGLPIFAIAAY
                     QLFNPPAGAGTSYLFIWLIVVYIGYSMLALAHSAWASRIAGAYHERSRLFGWMQCMAV
                     LGTSSILALPLVTGGRINPAVSTTMGAVGWVVIGVALVVVPLTLFAAHEPVTAEIKKE
                     RTTVQDY"
     gene            complement(779..>2049)
                     /locus_tag="VFU72_06285"
     CDS             complement(779..>2049)
                     /locus_tag="VFU72_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423129.1"
                     /translation="GLFGLVRLIDIPIDPALGLMMDRTRTPFGRYRPWIIVGIPILML
                     ALYMLYQAPVGVTRGTLMIWLLVMYLGTSILVLGVNSWASSLASTYQDRSRIFGIMSA
                     LGVAGSATVMLIPVVVGRVSGGGEAASVRAVGWFLAGLAPIAIGFAVLNTPERIVGGA
                     HAARFTLRDYLSLVTRPNVLRILAADLAVTLGPGWMAALYIFYWEASRRVSFGMSNLL
                     LVIYILAGLVGAPVTALLANRIGKHRALIVSTTLYSLFLIIIPLTPKGAWIPSVPIMF
                     CLGAFAAGFVVTIRAITADVGDEIRLEKGSHQMGLLYALTSATSKVAGAFSITFTFKV
                     LQMIGYDPKLGPANGPAQIHGLELAFIVGPIFFVMAGGACFLGYKLTADKHADIRRQL
                     DERDAVYAEPAAVEAATGGAMEPLATPPNA"
BASE COUNT          368 a          667 c          750 g          264 t
ORIGIN      
        1 gtagtcctgg acggtcgtcc gctccttctt gatctcggcc gtgaccggct cgtgggcggc
       61 gaacagggtc agcggcacga cgaccagcgc gaccccgatc accacccagc cgaccgcgcc
      121 catggtcgtg gagaccgccg ggttgatgcg gccgccggtg accagcggca gggccaggat
      181 ggacgaggtg ccgagcaccg ccatgcactg catccagccg aacagccgcg accgctcgtg
      241 gtaggcgccg gcgatccgcg aggcccaggc cgagtgcgcc agcgccagca tcgagtagcc
      301 gatgtagacc acgatcagcc agatgaagag gtagctcgtt ccggcgccgg ccggggggtt
      361 gaacagctga taggcggcga tggcgaagat cggcaagccg gccgcatacc agggccgata
      421 gcggccgaag cgcgtgtggg tcttgtccat cagccagccg agggcgaggt cgaagcccag
      481 gtcggtcagc cgcaccgtcg ccatcacccc gccgagggcc aggagggtca gcccgatgtg
      541 gccggcgtag aacttcggca ggtagacgcc gaccaggatc gccatgatgt agaccggcgt
      601 gctgccggtc gagaaggcca gcagccgcgg aaacggcagc gagcggcccc cggatgcgga
      661 gggggtggcg gtcaagagcg tctcctcaaa caatcaatgg tcgccctctg gcggggcgct
      721 aatcgggtca gggaagcgcg gcgcgtgagg cggcgcaagg catttccgtc gcgcaacatc
      781 aggcgttggg cggggtggcc aggggctcca tggcgccgcc tgtcgccgct tcgacggcag
      841 ccggttcggc atagacggcg tcgcgctcgt cgagctggcg gcggatgtcg gcgtgcttgt
      901 cggcggtcag cttgtagccc aggaagcagg cgccgcccgc catgacgaag aagatcggcc
      961 cgacgatgaa ggccagctcc agcccgtgga tctgggccgg gccgttggcc ggtcccagct
     1021 tggggtcgta gccgatcatc tgcagcacct tgaaggtgaa ggtgatcgag aaggcgcccg
     1081 ccaccttcga ggtggcgctg gtcagggcgt agagcaggcc catctggtgg ctgcccttct
     1141 ccaggcggat ctcgtcgccc acgtcggcgg tgatggcgcg gatcgtgacc acgaagccgg
     1201 cggcgaacgc gcccaggcag aacatgatcg gcaccgaggg gatccacgcg cccttggggg
     1261 tcagcggaat gatgatcagg aacagcgagt agagcgttgt cgagacgatc agcgcccggt
     1321 gcttgccgat ccggttggcg agcaaggcgg tgaccggcgc gcccaccagg ccggccagga
     1381 tgtagatcac caggagcagg ttcgacatgc cgaagctgac ccgccgcgag gcctcccagt
     1441 agaagatgta gagcgcggcc atccagcccg gcccgagggt gacagcaagg tcggcggcca
     1501 ggatgcgcag cacgttcggc cgggtcacca ggctgaggta gtcccgcagg gtgaagcgcg
     1561 cggcatgcgc gccgccgacg atcctctccg gcgtgttcag caccgcgaag ccgatcgcga
     1621 tcggcgcgag gcccgcgagg aaccagccca cggcgcgcac gctcgccgcc tcgccgccgc
     1681 cgctgacccg cccgaccacg accgggatca gcatcaccgt ggcggagccg gccaccccga
     1741 gcgccgacat gatgccgaag atccgcgagc ggtcctggta ggtgctggcc agcgacgaag
     1801 cccaggagtt gactcccagc accaggatcg aggtgccgag gtacatgacc agcagccaga
     1861 tcatcagcgt gccgcgggtc acgccgacgg gcgcctggta gagcatgtag agcgccagca
     1921 tcaggatcgg gatgccgacg atgatccagg gccgatagcg gccgaacggc gtgcgcgtgc
     1981 ggtccatcat cagcccgagc gcagggtcga tcgggatgtc gatcagccgc acgagcccga
     2041 acaggccgc
//
LOCUS       DASUBI010000326         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_831332, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000326 DASUBI010000000
VERSION     DASUBI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831332"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFU72_06290"
     CDS             <1..699
                     /locus_tag="VFU72_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HEU5423130.1"
                     /translation="VVRQGEREAHFWAPAPDWRNPENYIYNQIGQLRYGHDQPAAIEP
                     CGRGFRFTYQTVWRDDHDEPVLDETRTIAIYAGADATMCDVFTSKTATYGPLDYPASK
                     HGTIGVRIQPQLLPFMGGEILGGMEGTVRRGRADEVASGQPCNYVAYEADVPGLGRFG
                     VCLIVLANTASADHRGPWFIRDYGMAMFNASMRDAIHTAAGETWTAGLRVVAYDGALS
                     DERVRAWREPAATG"
     gene            742..>2459
                     /locus_tag="VFU72_06295"
     CDS             742..>2459
                     /locus_tag="VFU72_06295"
                     /inference="COORDINATES: protein motif:HMM:NF039329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423131.1"
                     /translation="MQTLPHIDVVGDARIQQVLDQPGNYLFAACACETLDGWVPDIGL
                     TNYPNESIFRPPSIAGAGISSTIRHRFWSTLDGETDHTGTRYIGVVLYDATGEECPLE
                     FRLGADDIGRIAPARHDNRRHLIVLDQPVAFHGEMEVLQVAAPGKGTYRIETFALLAA
                     RPAPSDFAPHLDRLTTRLVARDGDSVTAEVHCTTSQVAICEAVARHADAGEAAPLSAH
                     EDRPGRLHVMSLSGLAPDRQYTVYVTATEQAGATATARLPLFTGATQQDSGAASVTVP
                     VELLAPSDAPTLAGLPLTFGVPLPKGQLYAVDACQVRAGEVERPAQARVHARWPDGSA
                     RWVLIDTDCPSGLQPAITLPAQVTLTPAGAPISEEEPPALTWDIDAAGISVTGQTVRV
                     TVRRDQVRLAPQVERRGPDGAWGTVLTADEASAPTIVLGDGRELRAGAVTDLALEEAG
                     GERAAICYQVPHQDSAGVTHLLGTMRVQVYARQPFITLSHRLTVVSSELPPAAGGPAP
                     SGFKQGTALTAGAADEAQTLLTVRSATLHLPCADNRQVGFADEVYPLRETTSWRLGQG
                     DDRAYR"
BASE COUNT          409 a          835 c          826 g          389 t
ORIGIN      
        1 gtcgtgcgcc agggcgagcg cgaggcgcat ttctgggcgc ccgcgcccga ctggcgcaat
       61 ccagagaact acatctacaa ccagatcggc cagttgcgct atggtcacga ccagccggcg
      121 gccatcgagc catgcgggcg cggctttcgc ttcacctacc aaaccgtctg gcgtgatgac
      181 catgacgagc cggtactcga cgagacacgc acaattgcga tctatgccgg cgccgacgcg
      241 acgatgtgcg acgtctttac cagcaaaacc gccacgtatg gccctctgga ctatccggcc
      301 agcaagcatg gcacgattgg tgtgcgcatc cagccacaac tgctgccctt catgggcggc
      361 gagatcctcg gcgggatgga ggggactgtg cggcgcgggc gagcggacga agtggcctcc
      421 ggccagccgt gcaattacgt cgcctacgaa gcggatgtgc cgggtctcgg ccgcttcggc
      481 gtctgcctga ttgtgctggc gaacaccgcg tcggccgatc accggggacc atggttcatc
      541 cgcgactacg gcatggcgat gttcaacgcc tcgatgcggg acgccatcca caccgcagcg
      601 ggcgagacct ggaccgccgg cctgcgcgtc gttgcctacg atggtgcgtt gagcgacgag
      661 cgcgttcggg catggcggga accggcggcc accggttgat agtggtcacc cacccaatac
      721 ccatttacag gagccgcggc gatgcagacg cttccgcata ttgatgtcgt gggcgatgcg
      781 cgcatccagc aggtgctcga ccagccgggc aactacctgt tcgctgcgtg tgcctgcgaa
      841 acactcgacg gctgggtgcc ggacatcggc ttgaccaact accccaacga gagcatcttc
      901 cggccaccga gcatcgccgg cgccggcatc tcctccacga tccggcatcg cttctggagc
      961 acgctggacg gtgagaccga ccataccggc acgcgataca tcggcgtcgt gctctatgac
     1021 gcgaccggtg aggaatgtcc attggagttc cggctggggg ccgacgacat cggccggatc
     1081 gcgccagcgc ggcacgacaa ccggcgtcac ctcatcgtgc ttgatcagcc ggtcgcgttt
     1141 cacggcgaaa tggaggtcct gcaagtcgcg gcgcccggca agggcacgta ccgcatcgag
     1201 acattcgcgc tactggccgc gcgcccggcg ccgagcgact tcgcgccaca ccttgatcgc
     1261 ctcaccacgc gcctcgtcgc gcgtgacggg gacagcgtga cggcggaagt ccactgcacg
     1321 accagccagg tcgccatctg cgaagccgtg gcgcgacacg ctgatgctgg cgaagcagcg
     1381 cccctctcgg cgcacgagga ccggccgggc agattgcacg tcatgtccct gtctggcctg
     1441 gcgccggacc ggcagtatac cgtctatgtc acggctaccg agcaggctgg cgcgaccgcc
     1501 accgcgcggc tgccattgtt caccggggca acgcagcagg attccggcgc ggccagtgtc
     1561 actgtgccgg tggaactgct cgcgccttcg gacgcgccga cgctagctgg tctgccgctg
     1621 acctttggcg tgccgctgcc caaagggcaa ctgtatgcgg ttgacgcctg tcaggtccgg
     1681 gccggtgaag ttgagcgacc agcacaggcg cgcgttcacg cccgctggcc cgacggctcg
     1741 gctcgctggg tgctgattga caccgactgc ccgtcggggc tacagccagc tattaccttg
     1801 cctgcacagg tcacgcttac ccctgccggc gcgcccatca gtgaggaaga gccgccggca
     1861 ttaacttggg acattgacgc ggcgggcatc agcgtgaccg gacaaaccgt gcgcgtaaca
     1921 gtgcggcgcg atcaggtccg cctggcgccc caggttgagc ggcgcgggcc ggacggcgcg
     1981 tggggcaccg tgctcacggc ggacgaagca tccgcgccta ccattgtgct cggagatgga
     2041 cgggaactgc gcgcgggcgc ggtcaccgac ctcgcgctgg aggaggcggg cggtgagcgc
     2101 gcggccattt gctaccaggt gccgcaccag gatagcgccg gggtgacgca cctgctgggc
     2161 acgatgcgcg tgcaggtcta tgcccggcag cccttcatta ctctcagcca ccggctcacc
     2221 gtcgtcagtt ccgaattgcc gcccgcggca ggcggcccgg cgccgagcgg gttcaagcag
     2281 ggcacggccc tgaccgccgg cgctgctgac gaagcgcaga ccttgctgac tgtccggtcg
     2341 gccacgctgc acctcccctg tgccgacaat cggcaggtcg gctttgccga cgaggtctac
     2401 ccgctgcgtg agacgacatc gtggcggctg ggccagggag acgaccgggc gtaccggat
//
LOCUS       DASUBI010000327         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_650189, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000327 DASUBI010000000
VERSION     DASUBI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650189"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFU72_06300"
     CDS             <1..612
                     /locus_tag="VFU72_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU5423132.1"
                     /translation="DARSGREFVAPLPGSFQAVNAAAALGAARALALPALDDALIARG
                     LAATRFPGRIEVVQDSPQVVLDGAHNPDKMRSLAASVPLLGSPARRVLVLGALDGHDY
                     LQNARIIAPLCDAVVLTAPHTIERATASTEALAGVMRELGKPVDTVLEPREAIEAALA
                     QAGPEDQVLVTGSLYLVGQVRERWYPSDDIVWQQTSWPAALTP"
     gene            744..>2060
                     /locus_tag="VFU72_06305"
     CDS             744..>2060
                     /locus_tag="VFU72_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423133.1"
                     /translation="MPENPPLAIDELYELAGLPRDGNGDGYPDALRARIVLEDEPDAT
                     AWCALLDLAARLGLETAGFSPPLVVDEPGADQLPVVVRRGEAAEPRIVPGGFRGREAV
                     VVEGARALEALARAGQADRGALEAATAEPRRTLDLRDLFETSGLLADHDGDGAPDGTR
                     LCVVVPAALPRRLGAALVDLCVRLGMESGGVDLPLAVAGSPTAGSVPLAIELDPARQG
                     GELAVAPGEAGAGLVLRGDAGSAAALIERLTRGWPAPLPGRLDAAQVVTWLRRSVAGW
                     TAEGRAAALRVALLERGQLPEDAAIRLLTEDHVEARRLSTVARDASGVVVRGPGSSLT
                     VLAEEWSARWEAERALEALRERLLPGLDVALPLQLTVLVSEPATVRARLREQVERLLA
                     RAGFSPAQTRVVVLDAFKAGLSWLLEDVLPRLRELAGLDRLVIRYRP"
BASE COUNT          218 a          717 c          840 g          285 t
ORIGIN      
        1 gacgcccgct cgggccgcga gttcgtcgcg ccgctgccgg ggagcttcca ggcggtcaac
       61 gcggcggcgg cgctcggcgc ggcgcgcgca ctcgctctgc cggcgctgga tgacgcgctg
      121 atcgcgcgcg ggctggcggc cacccgcttt ccggggcgca tcgaggtggt gcaggactcg
      181 ccgcaggtgg tgctcgacgg cgcgcacaac ccggacaaga tgcgctcgct ggcggccagc
      241 gtgccgctgc tcggctcgcc cgcgcggcgg gtgctggtgc tgggcgcgct cgacggccat
      301 gattacctgc agaacgcgcg catcatcgcg ccgctctgcg acgcggtcgt gctgaccgcg
      361 ccgcacacca tcgagcgcgc caccgcctcg accgaggcgc tggccggggt gatgcgcgag
      421 ctgggcaagc cggtggacac cgtcctggag ccgcgcgagg cgatcgaggc cgcgctggct
      481 caggccgggc cggaggacca ggtgctggtg accggctcgc tctacctggt gggtcaggtg
      541 cgcgagcgct ggtaccccag cgacgacatc gtctggcagc agacgagctg gccagcggcc
      601 ctcaccccct gaccccctct cccaatgctg ggcgaggggg aacgagggcg ggcgggtttc
      661 aaacccgccc ctacagtcgt ccttgctccc ctctccaaat tttccgggag agcggctggg
      721 agtgagggcg cgaaggagca attgtgcccg agaacccgcc tctggcgatc gacgagctgt
      781 acgagctggc gggcttgccg cgcgacggca acggcgacgg ctatcccgac gcgctgcgcg
      841 ctcgcatcgt gcttgaggac gagccggacg cgacggcctg gtgcgccctg ctcgatctgg
      901 ccgcgcggct ggggctggag acggccggct tctcgccgcc gctggtagtg gacgagccgg
      961 gggcggacca attgccggtc gtagtgcggc ggggcgaggc ggccgagccg cgcatcgtgc
     1021 cgggcggctt ccgcgggcgt gaggcggtcg tcgtcgaggg cgcccgcgcg ctcgaggcgc
     1081 tggcgcgtgc cgggcaggcg gaccgtggcg cgctggaggc cgcgacggcg gagccccgcc
     1141 gcacgctcga tctacgcgat ctcttcgaga cgagcgggct gctggccgac cacgacggcg
     1201 acggcgctcc cgacggcacc cggctgtgcg tggtcgtgcc ggccgcgctg ccgcgccggc
     1261 tcggcgccgc gctggtcgat ctctgtgtcc ggctggggat ggagagcggg ggagtcgatc
     1321 tcccgctggc cgtggccggc tccccgacgg ccggctccgt tccgctcgcc atcgagctgg
     1381 atccggcgcg gcaggggggc gagctggcgg ttgcgccggg tgaggccggc gccggcctgg
     1441 tgctgcgcgg cgacgccgga tccgccgccg ccctgatcga gcgtctgacg cgcggctggc
     1501 cggcgccgct gccgggccgg ctggatgccg cgcaggtcgt gacctggctg cggcgctccg
     1561 tggccggctg gacggcggaa gggcgcgcgg ccgcgctgcg cgtggcgctg ctggagcgtg
     1621 gccagctccc ggaggatgcg gcgatccggc tcctgaccga ggatcacgtc gaggctcgcc
     1681 gcctctcgac ggtggcgcgc gatgcctccg gtgtggtcgt gcgcggcccg gggtcgagcc
     1741 tgacggtcct cgccgaagag tggtcggcgc gctgggaggc cgagcgcgcg ctggaggcgc
     1801 tgcgcgagcg gctgctgccg gggctggatg tggcgctgcc gctgcagctg acggtgctgg
     1861 tctcggagcc ggcgacggtg cgcgcccgcc tgcgcgagca ggtcgagcgg ctgctggcgc
     1921 gggccggctt ctccccggcg cagacgcgcg tggtggtgct cgacgccttc aaagcgggcc
     1981 tgagctggct gctggaggac gtgctgccac ggctgcggga gctggcaggg ctcgatcgcc
     2041 tggtgatccg ctaccggccg
//
LOCUS       DASUBI010000328         7809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1004852, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000328 DASUBI010000000
VERSION     DASUBI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004852"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1068)
                     /locus_tag="VFU72_06310"
     CDS             complement(145..1068)
                     /locus_tag="VFU72_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5423134.1"
                     /translation="MSATRTFLERKYGAPVDGVEPIGTGAWSQAFAYSIGDTRYVIRW
                     SDVADNFERDAFAARFNGAGLPVPPVNELGRSEDRFFAISPFVPGVYLESLADAELEA
                     TVPAILDMFRALRSVDLSGTTGFGFFDGSGNAHYPNWRAFLLNDKNESAGSLINGWRA
                     NLEASPLGAGDYDRLRARFEPLVERCPEVRGLVHADLLNYNVLCQGGRITGVLDWGSA
                     FYGDPLYDIVRSRFYAPWYPEFARIQLADQLLADFRADPRADTVEIELRLRCYELHIG
                     LDSIAYNAFKQDWAHAKEAAAYTLELTAPGR"
     gene            complement(1335..2096)
                     /locus_tag="VFU72_06315"
     CDS             complement(1335..2096)
                     /locus_tag="VFU72_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423135.1"
                     /translation="MSREARAAAPTSVPAAPGPLLGISRLNVAYGDVQVLWDVSLEVL
                     SGEIVALIGSNGAGKSTLLTTISGLLAPRSGAIVFAGAPITRAATQEIVRRGIAQVPE
                     GRRLFGAMTVRDNLLMGAFQREDKEQVQDDLARVLDLFPRLKERLSAQASKLSGGEQQ
                     MVALGRGLMSRPRLLMIDELSLGLAPVIVENLLEIIRQINRDGVTVLIVEQDVQIALE
                     IADRGYVLETGHITLAGPAAQLLADERVRQAYLGL"
     gene            complement(2089..3108)
                     /locus_tag="VFU72_06320"
     CDS             complement(2089..3108)
                     /locus_tag="VFU72_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5423136.1"
                     /translation="MSAPADGVILQVRDATKLYGGVMAVQDISFDVARGEILGLIGPN
                     GAGKTTLVNLITGTARPTRGTVVFEGAALHRLAPHKIGRLGIARTFQVVRPFLHLSVR
                     ENVAVGAMFGSGGARRSTGAAFRKADEVLDFVEMLPLRDEPADSLPIGWRKRLELAKA
                     LAMEPRLLLLDEVMAGLRGSEVDQAIALVKRINAGGITIVVIEHVMKVIMSLSHRVIV
                     LDYGKKIAEGTPAQVVRDPAVIQAYLGKKFAQTAGPATMDEAIDEEQAAARAAGLELD
                     EPPKPQEEAQALRTDDHPRGWQELGERQPGRAPGEEPGTAGQEAPEPDPPPGRAGEPK
                     ERERE"
     gene            complement(3105..4178)
                     /locus_tag="VFU72_06325"
     CDS             complement(3105..4178)
                     /locus_tag="VFU72_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5423137.1"
                     /translation="MNLLIFALALVLAIVAARPQLAPGLRRAGLVLLLAVMLAAPFIS
                     QSPAPLFDALTAMTVAYGWNFIGGYTGYPSFGNVAFLGLGAYIAAGFMSRQPHHPHLP
                     WYAGIPLGVILTTLAAVLIGLVVLRLRGHYFAIATLGVAVAMPQLINWSGLFGQFFGG
                     GLPLNFPLVRGASLFGLTLSADRFFYYLALGSALLSLGLTAWLSRSRFGYSLIAIREN
                     EEAAEVMGINTTVAKVMAYALSAALAALGGAIIGFRLTNMTTENSAIFNPINNLQMII
                     IVLIGGVATIWGPWIGAVILFGIQELIQTAAGGILDWQGVIFALIIIVVVIFLPAGLM
                     EFVRSRARFSWRLLAHNIAEHRV"
     gene            complement(4175..5041)
                     /locus_tag="VFU72_06330"
     CDS             complement(4175..5041)
                     /locus_tag="VFU72_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5423138.1"
                     /translation="MQFVQHIVNGLLGGFVLALVGLGFSMVWGILNIINLAHAAFIML
                     GAFTTYYLWERIGLDPFLSLPLTMIVIFIIGFLVQFYVINLVIRGSLLVTFLLTFGIE
                     GLAINLALRFFSANTRQTRPFYANESMGLGPIRVSYTLLAGAVVTLLLTLALYLFLDY
                     TKTGRSIRAVGLDQAAARLMGVDIGRTYALTFGIGAALAAAAGALISTANGFSPGSFD
                     AYNVLAFSVVVLGGLGSIPGALLGGLAFGMVNEFASVYALPQRDVVVFTILILVLVLK
                     PTGLLGREGYNL"
     gene            complement(5123..6529)
                     /locus_tag="VFU72_06335"
     CDS             complement(5123..6529)
                     /locus_tag="VFU72_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5423139.1"
                     /translation="MRSVRPVGLFLSLTLAALLVLAACGGSSSNSTTTSGATTAATSA
                     ATSAAGAATTVASPAATKASSGAASPAATSAAAGSPASGSFSGTFDGTILFGAAISLT
                     GATSKEGGYTRDGYELWKDTYNANGGIVINGKHYKIETEYYDDTSNAQTAATLADKLI
                     KEDHVQFLLGPYGTSSTLQVSTVAEKNQIPMVDTNGAAKSIFSQGYQYTFGVLSPAPN
                     YLQGVIDMALAQNPKPKSVAILSADDPFSVEVADAAQKYAQSKGLEVVYYQKYPNAST
                     NLTAPLTEAKGKNPDLLLNSGHLEESVAIMQQAHQLDLNPLGYGFSVGPSLPDFQSTL
                     KNDANYVFGGTQWTPQLTYNADDLFKSPANYAKLFQQRFGAEPPYQAAESTAGGVTFV
                     KAIEKAGSVDPKKVRDALAGLSFTSFYGPIKFDAQGMNVTKPMAVEQWQNGQKQTVWP
                     QDVAAAKPMWPTPHWNQR"
     gene            complement(6817..7641)
                     /locus_tag="VFU72_06340"
     CDS             complement(6817..7641)
                     /locus_tag="VFU72_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5423140.1"
                     /translation="MSYDPTSTAGVPAHGRERLERMRAEVARGFFTSDLSVNEFLLVK
                     QAGFDPLGLVVGSSIYHIGYQQSMWSQNQEMGVLTQAMYHARELAMTRMEEEADQLGA
                     DGVVGVRLDIGRYEWGEALAEFIAIGTAVKHRGGELHRAPNGRPFTSDLSGQDFWTLL
                     SAGYRPVGMVMGNCVYHVAHQGLRNWFKQIGQNQEMPNYTQALYDARELAMERMQAEA
                     EELQSEGIVGVQIHERSHGWGSHVIEFFAVGTAVVPTTSEHQIAAPSMVLTLNDKG"
BASE COUNT         1233 a         2630 c         2706 g         1240 t
ORIGIN      
        1 gcgccggatg agaaggtcat gctgcagcag acgctggccg tggccggcca cggcctgacc
       61 cgcagcgaaa ccctgcgtgc ctatccgatc gaggaggccc agcagatgct gcagggctga
      121 tcgcccggac ccgctcagcc ggtatcagcg cccgggcgcg gtcagctcca gcgtgtaggc
      181 ggccgcctct ttggcatgcg cccagtcctg cttgaaggcg ttgtaggcga tcgagtcgag
      241 cccgatatgc agctcgtagc agcgaagccg cagctcgatc tccacggtgt cggcgcgcgg
      301 gtcggcgcga aagtccgcca gcagctgatc cgcgagctgg atacgcgcga attcagggta
      361 ccagggcgcg tagaaccggg accggacgat gtcgtacagg gggtcgccat agaacgcgga
      421 gccccagtcg agcaccccgg tgatgcggcc accctggcag aggacgttgt agttgagcag
      481 gtcggcgtgg accagcccgc gcacctccgg gcagcgctcc accagcggct cgaaacgcgc
      541 gcgcagccgg tcgtagtccc cggcgccgag cggggatgct tcgaggtttg cgcgccagcc
      601 gttgatcagg ctgccggcag actcgttctt gtcgttcagc aggaacgcac gccagtttgg
      661 gtagtgcgca ttgccgctgc cgtcgaagaa gccgaagccg gtggtgcctg agagatcgac
      721 cgatcgcagc gcgcggaaca tatcgaggat ggcgggaacc gtcgcttcga gctccgcgtc
      781 agcgagcgac tccagataga cgccgggcac gaagggggag atggcgaaga accgatcctc
      841 cgaccggccg agctcgttga ccggcggcac tggcaagccc gcgccattga agcgcgcggc
      901 gaacgcgtcg cgctcgaagt tgtccgcgac atcgctccag cggatgacgt acctggtatc
      961 accgatggaa tacgcgaacg cctgcgacca ggcgccggtg ccgatcggct cgacaccgtc
     1021 gaccggtgcg ccgtatttcc gctcgagaaa cgtccttgtc gccgacactg tcgcgctcgt
     1081 cattgcggcc tttccagcgc cgtttccata ccggggcgca cgagcgcggg cgatgggtgc
     1141 gggccgacga tcatgttgga tgccgctccg cgacggatcc ttcgactacg ctcgggatga
     1201 caagggtaag ggatggggcc tatcggacga ggatcgtctt cctcgtcagc ccctaaccaa
     1261 acactgtcat cctgagcgca gtcgaaggat ccgtcctcgc ccaccacctg accatgtcgc
     1321 tggccggcgc cctcctacag ccccagatac gcctggcgca cgcgctcgtc ggccaggagc
     1381 tgggcggcgg ggccggcgag cgtgatgtgg ccggtctcca gcacgtagcc gcggtcggcg
     1441 atctccagcg cgatctggac gtcctgctcc acgatcagca ccgtcacccc gtcgcggttg
     1501 atctgccgga tgatctccag caggttctcg acgatcaccg gcgccagccc cagcgacagc
     1561 tcgtcgatca tcagcaggcg cggccggctc atcaggcccc ggcccagcgc caccatctgc
     1621 tgctcgccgc ccgagagctt actcgcctgc gcgctcagcc gctccttgag gcgggggaag
     1681 aggtcgagca cgcgcgccag atcgtcctga acttgctcct tgtcctcgcg ctgaaaggcg
     1741 cccatcagca ggttgtcgcg cacggtcatg gcgccgaaca ggcggcggcc ctcgggaacc
     1801 tgggcgatgc cccggcgcac gatctcctga gtggcggcgc gagtgatcgg cgccccggcg
     1861 aagacgatcg cgcccgagcg cggggcgagc aggccgctga tggtcgtcaa cagcgtgctc
     1921 ttgccggcgc cgttggagcc gatcagcgcc acgatctcgc cggacagcac ctccaggctg
     1981 acgtcccaga gcacctgcac atcgccgtag gccacgttga gccggctgat ccccagcagc
     2041 gggccggggg cggcgggcac agacgtgggt gccgccgcgc gcgcctccct actcacgctc
     2101 gcgctccttc ggctcgcccg cgcgaccggg cggcggatcc ggctctggcg cctcctgccc
     2161 ggccgtgccc ggctcctcgc ccggggcgcg gccgggctgc cgctcgccca gctcctgcca
     2221 gccgcgcggg tggtcgtcgg tgcgcagcgc ctgcgcttcc tcctgcggtt tgggcggctc
     2281 gtccagctcc agcccggcgg cacgggccgc cgcctgctcc tcatcgatgg cctcgtccat
     2341 ggtcgccggc ccggccgtct gggcgaactt cttgccgaga taggcctgga tcaccgccgg
     2401 gtcgcgcacc acctgggcgg gggtgccctc ggcgatcttc ttgccgtagt cgagcacgat
     2461 cacgcggtgg ctcaggctca tgatcacctt catcacgtgc tcgatgacga cgatggtgat
     2521 gccgccggcg ttgatgcgct tgaccagtgc gatcgcctgg tcgacctcgc tgccgcgcaa
     2581 tcccgccatc acctcgtcga gcagcagcag gcgcggctcc atcgccagcg ccttagccag
     2641 ctccaggcgc ttgcgccagc cgatcggcag gctgtcggcc ggctcgtcgc gcagcggcag
     2701 catctcgacg aagtcgagca cctcatccgc cttgcggaag gcggcgccgg tcgagcgccg
     2761 cgcgccgccg ctgccgaaca tggcgccgac cgccacgttc tcgcgcacgc tgagatggag
     2821 gaaggggcgc accacctgga aggtgcgcgc gatccccagg cggccgatct tatgcggcgc
     2881 caacctgtgc agcgcggccc cctcgaagac gaccgtgccg cgcgtgggcc tggcggtgcc
     2941 ggtgatcagg ttgaccagcg tcgtcttgcc ggcgccgttg gggccgatca gacccaggat
     3001 ctcgccgcgc gccacgtcga agctgatgtc ctgcaccgcc atgacgccgc cgtagagctt
     3061 ggtggcgtcg cgcacctgca aaatcacgcc atcggccggc gcgctcatac acggtgctcg
     3121 gcgatgttgt gggccagcag ccgccagctg aagcgcgccc ggctgcgcac gaactccatc
     3181 agcccggcgg gcaggaagat gacgacgacg atgatgatca gcgcgaagat caccccctgc
     3241 cagtcgagga tgccgccggc cgcggtctgg atcagctcct ggatgccgaa gaggatcacg
     3301 gcgccgatcc agggccccca gatggtggcc acgccgccga tcaggacgat gatgatcatc
     3361 tgcaggttgt tgatcgggtt gaagatggcc gagttctcgg tcgtcatgtt ggtcaggcgg
     3421 aagccgatga tcgcgccgcc gagcgcggcc agcgcggcgc tgagcgcgta ggccatcacc
     3481 ttggccacgg tggtgttgat ccccatcacc tcggccgcct cctcgttctc gcggatggcg
     3541 atcaggctgt agccgaagcg gctgcggctg agccaggcgg tcagacccag gctgagcagc
     3601 gccgagccga gcgccaggta gtagaagaag cggtcggcgc tgagcgtcag gccgaagagg
     3661 ctggcgccgc gcaccagtgg gaagttcagc ggcagcccgc cgccgaagaa ctgcccgaag
     3721 agtccgctcc agttgatcaa ctgcggcatc gcgaccgcca cgccgagggt ggcgatggcg
     3781 aagtagtggc cgcgcaggcg cagcaccacc agcccgatca acaccgccgc cagggtggtc
     3841 aggatcaccc ccagcgggat gccggcgtac caggggaggt ggggatggtg cggttggcgg
     3901 ctcatgaagc ccgccgcgat gtaggcgccc agcccgagga aggcgacgtt gccgaaggag
     3961 ggatagccgg tgtagccgcc gatgaagttc cagccgtagg ccacggtcat ggcggtgagc
     4021 gcgtcgaaga ggggcgccgg gctctggctg atgaagggcg cggccagcat caccgccagc
     4081 agcagcacca gtcccgcgcg ccgcaggccg ggcgccagct gcgggcgggc ggccacgatc
     4141 gccagcacca gagccagcgc gaagatgagg aggttcatag gttgtagccc tcccgcccga
     4201 gcagcccggt cggcttgagc accagcacca ggatgaggat ggtgaagacc accacgtcgc
     4261 gctggggcag cgcgtagacg ctggcgaact cgttgaccat gccgaaggcc agcccgccga
     4321 gcagcgcgcc ggggatgctc cccagcccgc cgagcacgac cacgctgaag gccagcacgt
     4381 tgtaggcgtc gaaggagccg ggtgagaagc cgttggcggt cgagatcagc gccccggcgg
     4441 cggcggccag ggcggcgccg atgccgaacg tgagcgcgta ggtgcggccg atatcgaccc
     4501 ccatcagccg cgccgccgcc tggtcgagcc cgacagcccg gatcgagcgc ccggtcttgg
     4561 tgtagtccag gaagaggtag agcgccagcg tcagcagcag ggtaaccacg gcgccggcca
     4621 gcagcgtgta ggagacgcgg atcgggccga ggcccatcga ctcgttggcg tagaagggcc
     4681 gggtctggcg cgtgttggcc gagaagaagc gcagcgccag gttgatcgcc agcccctcga
     4741 tgccgaaggt caagaggaag gtgaccagca ggctgccgcg gatgaccaga ttgatcacgt
     4801 agaactgcac caggaagccg atgatgaaga tcacgatcat ggtgagcggc aggctgagga
     4861 aggggtccag cccaattcgc tcccaaagat agtaggtggt gaaggcgccg agcatgatga
     4921 aggcggcgtg ggccaggttg atgatgttca ggatgcccca gaccatcgag aagccgagac
     4981 ccaccagcgc caggacgaac ccgccgagca ggccgttgac gatgtgctgc acaaactgca
     5041 tcgcgactct cccccgatcg ccggcgatga tcaggcgccg ggcctgagac agctcagacc
     5101 cggcgcggca ggcgacgcgc gcctagcgtt ggttccagtg cggcgtcggc cacatcggct
     5161 tggcggcggc gacatcctgc ggccagaccg tctgcttctg gccgttctgc cactgctcga
     5221 ccgccatcgg tttggtgacg ttcatcccct gcgcgtcgaa cttgatcggc ccgtagaacg
     5281 aggtgaagct gaggccggcc agcgcgtcgc gcaccttctt gggatcgacg ctgcccgcct
     5341 tctcgatcgc cttgacgaag gtcacgccgc cggcggtcga ctcggccgcc tggtagggcg
     5401 gctcggcgcc gaagcgctgc tggaagagct tggcgtagtt ggccggcgac ttgaagagat
     5461 catcggcgtt gtaggtcaac tgcggggtcc actgggtgcc gccgaagacg tagttggcgt
     5521 cattcttcag cgtcgactgg aagtcgggca gcgacgggcc gacgctgaag ccgtagccca
     5581 gcgggttgag gtcgagctgg tgcgcctgct gcatgatggc gacgctctcc tccaggtggc
     5641 cgctgttgag cagcaggtcg gggttcttgc ctttggcctc ggtcagcggc gcggtcaggt
     5701 tggtggaggc gttggggtac ttctggtagt agacgacctc cagccccttg gactgcgcgt
     5761 acttctgcgc cgcgtcggcc acctcgaccg agaacgggtc gtcggcgctg aggatggcga
     5821 ccgacttcgg cttgggattc tgcgccagcg ccatgtcgat cacgccctgc aggtagttgg
     5881 gcgccgggct gagcacgccg aaggtgtact gatagccctg gctgaagatg ctcttggccg
     5941 ccccgttggt gtcgaccatc gggatctggt tcttctcggc cacggtgctg acctgcagcg
     6001 tcgagctggt gccgtagggg ccgagcagga actggacgtg gtcttccttg atcagcttgt
     6061 cggccagggt cgcggccgtc tgggcattgc tggtgtcgtc gtagtactcg gtctcgatct
     6121 tgtagtgctt gccgttgatc acgatgccgc cgttggcgtt gtaggtgtcc ttccagagct
     6181 cgtaaccgtc gcgggtatag ccgccctcct tggaggtggc gccggtcagc gagatcgccg
     6241 cgccgaagag gatcgtgccg tcgaaggtgc cgctgaagct gccgctggcc ggcgagccgg
     6301 cggccgcgct cgtggcggcg gggctcgcgg caccgctgga cgccttggtg gcggccggcg
     6361 aggcgaccgt ggtggcggcg ccggccgcgc tggtggccgc gctggtggcg gccgtggtcg
     6421 cgccgctggt ggtagtggaa ttcgacgatg agccgccgca ggccgccagc accagcagcg
     6481 cggccaacgt gaggctgagg aacaagccaa caggccgtac gctccgcacg gatctcctcc
     6541 tcacccggtg aacccgagtc cactggtaat ccccctgagc caagtgacag gagcggtatg
     6601 ccggtcagtc tcgcgtgctg ggttgtcgcg ccacatggcg ataccgcgtc tccgccgcgc
     6661 cgaggagcac gcgccgcccc gaacgatctc ctcctcactc tgcgccttcg cgatccctcg
     6721 cacatccccc gtcgtgcgcg gatgccgcat atcgacttcc gaggttggct cagtgggcgc
     6781 atcgtacacg cccgggcggc gacgcacaag cgcgggctag cccttgtcgt tcagcgtcag
     6841 caccatcgac ggcgcggcga tctggtgctc ggacgttgtc ggcacgaccg cggtgccgac
     6901 ggcgaagaac tcgatgacgt gcgagcccca gccgtggctg cgctcgtgga tctgcacccc
     6961 gacgatcccc tcggactgca gctcctcggc ctcggcctgc atgcgctcca tggccaattc
     7021 ccgggcgtcg tagagcgcct gggtgtagtt cggcatctcc tggttctggc cgatctgctt
     7081 gaaccagtta cgcaggccct gatgcgccac gtggtagacg cagttgccca tcaccatgcc
     7141 gaccggacgg tagccagccg agaggagcgt ccagaaatcc tgccccgaga ggtcgctggt
     7201 gaaggggcgg ccgttgggcg cgcggtgtag ctcgccgccg cggtgcttga cggccgtgcc
     7261 gatggcgatg aactcggcca gcgcctcgcc ccactcgtag cgcccgatgt cgagccgcac
     7321 gccgaccacg ccgtcggcgc cgagctgatc ggcctcctcc tccatgcggg tcatcgccag
     7381 ctcgcgggcg tggtacatcg cctgggtcag cacccccatc tcctgattct ggctccacat
     7441 cgactgttga tagccgatgt ggtagatcga gctgccgacc actagcccca gcggatcgaa
     7501 gcctgcctgc ttgaccagca ggaactcgtt gaccgagagg tcgctggtga agaagccgcg
     7561 cgccacctcg gcccgcatgc gctccagccg ctcgcgccca tgcgccggca ctcccgccgt
     7621 cgaggtcgga tcgtagctca tcgtgcgctt cccttctcac taccgcgcaa ctggttgccg
     7681 ctggcgccac tcgactccgc gcgcagatcg agcaccgggt ggatcgccgg gatcttcggg
     7741 accatctcct tcagtgcggt ccccatgacg tggaaggtga cgatcatgtc tttgcgcttc
     7801 tggtcattg
//
LOCUS       DASUBI010000329         7931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1037708, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000329 DASUBI010000000
VERSION     DASUBI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037708"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFU72_06345"
     CDS             complement(<1..1115)
                     /locus_tag="VFU72_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HEU5423141.1"
                     /translation="MTRRYLSAAEAASELGIAQATLYAYVSRGLIHSEPADSGNGHSR
                     ARRYRAEDVRRLRERQEQRHDPARAAATALSWGSAVLESAITLIHDGRLYYRGRDALA
                     LAHSRSIEEVAALLWTGALDQPLDWPAGAPLAPQPLEPTLPAIERCQLLLPRLQAADP
                     RAYDLQPRALLAAGARILRALVREAAGGVPVEGGSAATLQRAWAPGEPGAAALLNAAL
                     VLSADHELNVSSFTVRCVASAGAPLYAAVLGGLSALEGVKHGGVSRRVEALLREIGEP
                     GNARAVLQSRLRRGEPIPGFGHRLYPEGDPRGALLLELSRQAFPDTEAVRLVDATVAA
                     GRELLDERPNIDLGLVGLTRALGLPAGSALTLRALGRV"
     gene            1276..2430
                     /locus_tag="VFU72_06350"
     CDS             1276..2430
                     /locus_tag="VFU72_06350"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HEU5423142.1"
                     /translation="MSAATSQSTAFRAGLEGVIAAVTRLSDVDGQAGELILRGFPLEE
                     IASRANFEELVYLFWHGQLPNAAQLADFQAALAAQRELPEATQALLAAAAARKLPPMD
                     ALRVAVDSLSLELASASGDLAVATGLLARAPVIVAAYSRLLAGLPPVAPDPALGHAAN
                     YLFMLSGEPPSAARVRALDTYLNTVIDHGLNASTFAARVIVATQSDLVSAIVGALGAL
                     KGPLHGGAPGPALDMVFEIGSVERAEPYLRAKLERGERLMGFGHRVYKTRDPRADVLA
                     AAAARLFEAGAGDPQLYALVRAVEERALALLEEYKPGCHLRTNVEFYTALLLHALELP
                     VELFTPTFAVSRTAGWLAHCFEQQAANRLIRPLSAYDGPRDRRWVAIEAR"
     gene            complement(2553..3470)
                     /locus_tag="VFU72_06355"
     CDS             complement(2553..3470)
                     /locus_tag="VFU72_06355"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5423143.1"
                     /translation="MTGQEPRRPAGKMHAGELDIDAALVRRLLAAQFPQWAELPLAPV
                     PSAGTDNALYRLGAELAVRLPRIQEATAQVEKEQRWLARLAPQLPLAIPVPLALGAPS
                     AGYPWPWSVYRWLDGENATRERLADPAQAATELAGFLAALRRIDPGGGPSPGAHNSFR
                     GAPLARRDASVREAIVALRGMLDAEAVTAVWEAALQAPEWGDPPVWIHGDLLPGNLLV
                     VAGRLSAVIDFGCLGVGDPACDLMSAWALFSGESREAFRAQLAVDDASWARGRGWALS
                     WALIFIPYYLETNPTGVRAAWHTLAEVLA"
     gene            complement(3467..4666)
                     /locus_tag="VFU72_06360"
     CDS             complement(3467..4666)
                     /locus_tag="VFU72_06360"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEU5423144.1"
                     /translation="MTATRLLVAQSGGATAVINSSLVGVVEAARAAGLDVLGARRGIE
                     GILAGDLVDLGRQPQTVLDHLRRTPSAALGTTRQRLDDDSAARAAALLARQGIRLLAY
                     IGGNDSADTALRLAAAARAAGQELAVVGVPKTIDNDLPGTDHCPGYGSIARFMALAAR
                     DAGRDTEASAGLHPIKLIEVMGRDAGWITAAAALGQESPAEAPQLIFFPERPARDLDG
                     FVAEIAAAYRTYGQVVAVVPETIKDASGKPLAGSVSWRDSFGHPYTPSPGPALAQAIQ
                     ERLGARARFDKPGTIARMFMAAVSEPDLAEAYAVGAGAVRLLLRGVSEVMVTLERVSD
                     EPYQVRTGSVPLPEVANRERLLPDEFIAPGGRAVTDAFRRYALPLIGGPLPPYARLLD
                     LAPGESR"
     gene            4789..4938
                     /locus_tag="VFU72_06365"
     CDS             4789..4938
                     /locus_tag="VFU72_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423145.1"
                     /translation="MVEQIGDLIRRGAEALRDFIDPHGRLARRPGYAPVPVPVRTRHY
                     PRRYR"
     gene            complement(5262..6314)
                     /locus_tag="VFU72_06370"
     CDS             complement(5262..6314)
                     /locus_tag="VFU72_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423146.1"
                     /translation="MASTRLMRIVAIVSLAAALLAFAPVAAAKGPAAPTGPQVRRYLV
                     PPNGQPGGQRQVKPRDRVQQGGTAGASAPLSQGILDQSQTSFFDGGGLSVDQVQIVGQ
                     VFTPELSGALSAARLQVGRCVQYDGSDQPLPVGDLIAEIRTVDPRTGLPTATVLSTAL
                     VPEADVWQGSIWNGSQYVAFDDSTWTTIQFAQPATVAAGTPYALLLRPAAPSDCNFFW
                     SWGVGSGDLYPGGYLVSNFPSEPYADQWFRHDETDLAFQTYVTPLTPPEWIAGMKKQI
                     RDMQLPHTVATSLTAKLDATLASIEKEHTGPACNQLAAFINQVEAQRGKALTPEQADL
                     LISEAMATQSSLTCAR"
     gene            complement(6599..7321)
                     /locus_tag="VFU72_06375"
     CDS             complement(6599..7321)
                     /locus_tag="VFU72_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEU5423147.1"
                     /translation="MVLLLLFGLFVFAGFALLVLLATVGVTAASLVTLASAPAQLRAL
                     VRDRRLRRNHALEHATINVIEERFGPSRLSGQAGAEGFVIRGGVSPQVVAEAASEALE
                     RLRAGERRLAIHPRCGTTLLAAQLVLAVTFLAALLVFDSFSLLPFLLGILAAALLGPR
                     LSPLMQRWLTTDADMGALGVTAVEVRPPHAGLGLVSWLMLSPVTVRTGEGAELSETGP
                     RTGDHPRAAEPWEIPARDYRIR"
     gene            complement(7393..7587)
                     /locus_tag="VFU72_06380"
     CDS             complement(7393..7587)
                     /locus_tag="VFU72_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423148.1"
                     /translation="MAGEREINELVAELRRGLPATPASWSAEDLQRFCENIARAIVRA
                     GEQATAAGQQPEYGEVERPG"
     gene            complement(7702..7872)
                     /locus_tag="VFU72_06385"
     CDS             complement(7702..7872)
                     /locus_tag="VFU72_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423149.1"
                     /translation="MAVINKIIRLMGNGADNGFESYYTALARRQGGPNANEARRDYEQ
                     IRHILDKAFIIG"
BASE COUNT         1122 a         2879 c         2875 g         1055 t
ORIGIN      
        1 acgcgcccca gcgcccttag agtcagcgcg ctacccgccg gcaggccgag cgcgcgcgtc
       61 aggccgacca gccccaggtc gatgttgggc ctctcgtcga gcagctcgcg ccccgccgcc
      121 acggtcgcgt cgaccagccg caccgcctcg gtgtccggaa acgcctggcg ggacaactcc
      181 agcaggagcg cgccgcgcgg gtcgccctcg gggtagaggc ggtggccgaa gccggggatc
      241 ggctcgcccc ggcgcaggcg gctctggagg acggcgcgcg cgttacccgg ctcgccgatc
      301 tcgcgcaaga gcgcctcgac ccgccggctg accccgccgt gcttgacgcc ctccagcgcg
      361 ctcagcccgc ccagcaccgc cgcgtagagc ggcgcgccgg ccgaggccac gcagcgcacg
      421 gtgaaggatg acacgttcag ctcgtggtcg gccgagagca ccagcgccgc gttcaagagc
      481 gcggccgccc ccggctctcc cggcgcccac gcgcgctgca gcgtggcggc gctgccgccc
      541 tcgacgggca cgccgccggc cgcctcgcgc accagcgccc gcaggatgcg cgctccggcc
      601 gccagcagcg cccgcggctg caggtcgtag gcgcgcgggt cggccgcctg caggcgcggc
      661 aggaggagct ggcagcgttc gatggccggg agggtcggct ccaggggctg gggggcgagc
      721 ggcgcgccgg ccggccagtc gagcggctgg tcgagcgcgc cggtccagag gagcgcggcg
      781 acctcctcga tggagcgaga gtgggccagc gccagcgcgt cgcgcccgcg atagtagagc
      841 cgcccgtcgt ggatcagggt gatggccgac tcgagcacgg cgctgcccca actcagcgcg
      901 gtggcggccg cgcgggcggg gtcgtgccgc tgctcctggc gctcccgcag ccgccgcacg
      961 tcctccgccc ggtaacggcg cgcgcggcta tgaccattcc cgctgtcggc cggctcggag
     1021 tggatcaggc cgcggctgac gtaggcgtag agggtcgcct gggcgatgcc gagctcgctc
     1081 gccgcctcgg ccgcgctgag atagcgccgg gtcatcgtgc cgctcctctc cgctcagagc
     1141 ctgccgcttt gcggccaagg atgccatgca ttgatttatt gatcaagatt gacgcggcta
     1201 cttgaccgcg cctagactgg gccacgagcg gccgccgcgg cgtctcggcg gcccgagcaa
     1261 gccgaggagc gaactatgag cgcagccaca tcacagagca ccgcgtttcg agcggggctc
     1321 gagggcgtca tcgccgccgt cacccggctc agcgatgtcg acggccaggc gggcgagctg
     1381 atcctgcgcg gcttcccgct ggaggagatc gcctcccggg cgaacttcga ggagctggtc
     1441 tacctcttct ggcacggcca gcttcccaat gccgcgcagc tggcggactt ccaggccgcg
     1501 ctggccgccc agcgcgaatt gccggaggcg acgcaggcgc tgctggcagc ggccgccgcg
     1561 cgcaagctgc cgccgatgga cgccctgcgc gtggcggtcg acagcctcag tctggagctg
     1621 gcgtcggcct cgggcgacct ggccgtcgcg accggcctgc tcgcgcgcgc gccggtgatc
     1681 gtggccgcct acagccgcct gctggcgggc ctgccgccgg tcgcgcccga tccggcgctg
     1741 ggccacgccg ccaactacct cttcatgctg agcggcgagc cgccctccgc ggcccgcgtg
     1801 cgcgcgctcg acacctacct caacaccgtc atcgaccacg ggctgaacgc ctcgaccttc
     1861 gccgcgcgcg tgatcgtggc gacgcagtcg gacctcgtct cggccatcgt gggggcgctg
     1921 ggcgcgctca aggggccgct gcacggcggg gcgccggggc ccgcgctcga catggtcttc
     1981 gagatcggga gcgtcgagcg cgccgagcca tacctgcgcg ccaagctcga gcgcggcgag
     2041 cgcctgatgg gcttcggcca ccgcgtctac aagacgcgcg acccgcgcgc cgacgtgctg
     2101 gccgccgccg ccgcgcgcct cttcgaggcg ggcgcgggcg acccccagct ctacgcgctg
     2161 gtgcgggcgg tcgaggagcg ggcgctggcg ctcttggagg agtacaagcc gggatgccac
     2221 ctgcgcacca acgtcgagtt ctacaccgcg ctgctgctgc acgcgctcga gctaccggtc
     2281 gagctcttca cccccacctt cgccgtcagc cgcacggccg gctggctggc gcactgcttc
     2341 gagcagcagg cggccaatcg cctgatccgg cccctgtcgg cctacgacgg cccgcgcgac
     2401 cgccgctggg tggcgatcga ggcgcgctga gcagccgggc ggccctcatc cccccgcccc
     2461 ttctcgaacg gagcccaatg ctgggagaag gagagtcatg accgttcccc ctcgcccaga
     2521 attgggagag ggggttaggg ggtgagggcg cgtcaggcca ggacctcggc gagcgtgtgc
     2581 caggcggcgc gcacgccggt ggggttcgtc tccagatagt acgggatgaa gatcagcgcc
     2641 caggagagcg cccagccgcg cccgcgcgcc cagctcgcgt cgtcgaccgc cagctgcgcg
     2701 cgaaacgcct cccggctctc gcccgagaag agcgcccagg cgctcatcag gtcgcaggcg
     2761 gggtcgccca cgcccaggca gccgaagtcg atcacggcgc tgagccggcc cgcgacgacc
     2821 aggagattcc ccggtaacag gtcgccgtgg atccagacgg gtggatcgcc ccactccggc
     2881 gcctgcagag ccgcttccca gacggccgtg accgcttcgg cgtcgagcat ccctcgcagc
     2941 gcgacgatcg cctcccggac ggacgcgtcg cgccgggcca ggggcgcgcc acggaaggag
     3001 ttgtgcgcgc cggggcttgg cccgccgcct gggtcgatgc gccgcagggc cgccaggaag
     3061 cccgccagct ccgtcgccgc ttgcgccgga tcggccaggc gctcgcgggt cgcgttctcg
     3121 ccgtcgagcc agcggtagac ggaccagggc caggggtagc ccgcgctcgg tgctcccagc
     3181 gccagcggga ccgggatggc cagcggcagc tgtggggcga gccgcgccag ccagcgctgc
     3241 tccttctcca cctgcgccgt cgcctcctga atgcgcggca ggcgcacggc cagctccgcg
     3301 cccagccggt agagcgcgtt gtccgtcccg gcggagggga ccggtgcgag cggcagctca
     3361 gcccactggg gaaactgcgc ggcgagcaga cggcggacga gcgccgcgtc gatatccagc
     3421 tcgccggcgt gcatcttccc cgcgggccgg cgcggctcct ggccggtcat cggctttcgc
     3481 ccggcgccag gtcgagcagg cgggcgtagg gggggagcgg cccgccgatc agcggcagcg
     3541 cgtagcggcg gaaggcatcc gtcaccgcgc gcccgcccgg cgcgatgaac tcgtcgggca
     3601 gcagccgctc gcggttggcc acctcgggca gcggcaccga gccggtgcgc acctgatagg
     3661 gctcgtcgga gacgcgctcc agcgtgacca tcacctcgga gacgccgcgc agcagcagcc
     3721 gcaccgcccc ggcgccgacc gcgtaggcct cggccagatc cggctcggag accgccgcca
     3781 tgaacatgcg cgcgatggtg ccgggcttgt cgaagcgggc gcgcgcgccc aggcgctcct
     3841 ggatagcctg cgccagcgcc ggaccggggc tgggcgtata ggggtggccg aaggagtcgc
     3901 gccagcttac cgagccggcc agtggcttgc cgctcgcgtc cttgatcgtc tcgggcacca
     3961 cggccaccac ctggccatag gtccggtagg cggcggcgat ctcggccacg aagccgtcga
     4021 gatcgcgcgc cgggcgctcg gggaagaaga tcaactgcgg cgcctcggcg ggcgattcct
     4081 gccccagcgc ggccgcggcg gtgatccagc cggcgtcgcg ccccatgacc tcgatcagct
     4141 tgatcgggtg caggccggcg ctggcctcgg tgtcgcgccc cgcgtcgcgc gccgccagcg
     4201 ccatgaagcg ggcgattgag ccatagccgg gacagtggtc ggtgccgggg aggtcgttgt
     4261 cgatcgtctt gggcacgccg accacggcga gctcctgccc ggcggcgcgg gcggcggcgg
     4321 ccaggcgcag cgcggtgtca gccgagtcgt taccgccgat gtaggccagc agacggatgc
     4381 cctgacgcgc cagcagcgcg gcggcgcggg ccgcggagtc gtcgtccagc cgctggcggg
     4441 tggtgccgag cgcggccgag ggggtgcggc gcaggtggtc gagcacggtt tgcggctggc
     4501 gaccgaggtc gacgaggtcg ccggccagga taccctcgat gccccggcgc gcgccgagca
     4561 cgtcgaggcc ggctgcccgc gccgcctcga ccacaccgac caggctgctg ttgatgacgg
     4621 cggtcgcgcc gcccgattgc gccaccagca gcctcgtagc cgtcatccgc ttctcccctg
     4681 attgcgacga caaaatgcgc aactctcgcg cggcacgacc cgtgcgttat agcaagcagc
     4741 accggcagcg gcaaccgcgg ccggcgcgga gaggagtgac ggctcgcgat ggtcgagcag
     4801 ataggtgact tgattcgacg cggcgccgag gccctacgcg acttcatcga cccccacggt
     4861 cgcctggcgc gccgacccgg ctacgccccc gtgccggttc ccgtgcggac ccggcactac
     4921 ccacgccgct accgctagac cgatccagcg tatcccggtt ccgcgataac gtacgacggc
     4981 gggagaccgc cgagggcgaa tgcccccggc gtcgtcgtcc cccggcgcac ggccaggggc
     5041 cgtcgccaac gtttgctggc gtgtgaggac ggatccttgg accgcgctca gagcctgccc
     5101 tgagcttgcc gaagggatga ccggggggag ggatggggcc tagcggacga gacgagtcgc
     5161 cgtcgtccgc ccccaattaa ccctgtcatt ccggccgaag tggagggctc cgtgccgcac
     5221 gcggcaccca tgctcctctc ttcagccagc ggctcccgcg cctagcgcgc gcaggtaagg
     5281 ctggattgcg tcgccatcgc ctccgagatc agcaggtccg cctgctcggg cgtaagcgcc
     5341 ttgccccgct gcgcctccac ctggttgatg aaggccgcca gctggttgca ggccgggccg
     5401 gtgtgctcct tctcgatcga agcgagcgtc gcatcgagct tggcggtgag actcgtcgcg
     5461 acagtgtgcg gcagctgcat gtcgcggatc tgcttcttca ttcccgcgat ccactccggc
     5521 ggcgtgagcg gcgtcacgta ggtctggaag gcgagatccg tctcgtcatg ccggaaccac
     5581 tggtcggcgt agggctcgga cgggaaattg ctcaccaggt acccgcctgg gtagagatcg
     5641 ccgctgccga cgccccagga ccagaagaag ttacagtcgc tgggtgcggc agggcgaagg
     5701 agcagggcgt agggcgtgcc cgccgcgacc gtggccggct gggcgaactg gatcgtggtc
     5761 caggtggaat cgtcgaaggc gacgtactgg gatccgttcc agatcgaccc ctgccagacg
     5821 tccgcctcgg gcacgagggc cgtgctcagc accgtcgcgg tggggagccc ggtccgcggg
     5881 tcgaccgtgc gaatctcggc gatgagatcg ccgaccggca gcggttgatc ggagccgtca
     5941 tactgcacgc agcggcccac ctgcagccgc gcggcgctca gcgcccccga caattcgggg
     6001 gtgaacacct gccccacaat ctggacctga tcgaccgaca ggccgccgcc gtcgaaaaag
     6061 gacgtctgcg actgatcgag aatcccctgc gagagcggcg cggacgctcc ggccgttcct
     6121 ccctgctgca cccggtcccg cggcttcacc tgccgctgcc cgcccggctg gccattgggc
     6181 ggcacgaggt agcgccgcac ctgggggcca gtcggagccg ccggcccctt ggcggccgcg
     6241 acgggcgcga acgcgagcag cgcggccgcc agcgacacga tagccacgat ccgcatcaat
     6301 ctcgtcgatg ccacgagccc gttccttccc gtgtccgatc gagtccccca cgacgtcatc
     6361 ccgttcacag gcatggccaa cggtcaggtg gccggcgcgg cagtgaaagg gaggacgtca
     6421 gggcgaataa tagcacgaga gtacctgcta gtgtggtact tgtgggtgag gcattgaagt
     6481 tactgaggta cggcaatcgt gtcgccggca aagatcccag cccccagcga cgacgtgcgt
     6541 ctcgttaggg agcgggccac tcccggccgc cggacgggcg acaagcgccc gtccgcagct
     6601 agcggatacg atagtcgcgc gcggggatct cccagggctc ggcggcgcgg gggtggtcac
     6661 cagttcgcgg tccggtctcc gagagctccg cgccttcgcc ggtgcgcacc gtgaccgggc
     6721 tcagcatcag ccaggagacg aggcccagcc cggcgtgcgg cgggcgcacc tcgacggcgg
     6781 tcacgccgag cgcccccatg tcggcgtcgg tggtcagcca gcgctgcatg agcggactga
     6841 ggcgcgggcc cagcagggcc gcggccagga tgccgagcag gaaggggagg aggctgaagc
     6901 tgtcgaagac gagcagggcg gccaggaagg tgacggccag caccaactgc gcggccagca
     6961 gggtggtgcc gcagcgcggg tggatcgcca gccggcgctc cccggcgcgc agccgctcca
     7021 gcgcctcgga cgccgcctcg gccaccacct gcggcgagac cccgccgcgg atcacgaagc
     7081 cctcggcgcc ggcctgaccg gagagccgcg agggtccgaa gcgctcctcg atgacgttga
     7141 tggtggcgtg ctccagcgcg tggttgcgcc gtaggcggcg gtcgcgcacg agcgcgcgca
     7201 actgcgccgg cgcgctcgcc aaggtgacca gcgaggcggc ggtgacgccg accgtggcca
     7261 gcagcaccag cagcgcgaag cccgcgaaga cgaagagccc gaagaggagt agcaagacca
     7321 tggttccccg cgcttcttgc cggagagcga gtccggtccc gtaattatgt catattgggg
     7381 cggggcgacg ccttagcccg ggcgctcgac ctcgccgtac tcgggctgct gcccggccgc
     7441 cgtggcctgc tcgcccgcgc gcacgatggc gcgggcgatg ttctcgcaga agcgctgcaa
     7501 gtcctcggcg ctccagctgg ccggggtcgc cgggaggccg cgccgcagct ccgccaccag
     7561 ctcgttgatc tctcgctcgc ctgccacggt accctccgct cagatggtcg cctatcagca
     7621 ggagcatgat agccagccgc ccgcaaacgg gcaagcaaaa ggggctccat ggctggagcc
     7681 cctgccgccg gccgtcgccg gctagccgat gatgaaggcc ttgtcgagga tgtggcggat
     7741 ctgctcgtag tcgcgccgcg cctcgttggc gttcgggcca ccctggcgcc gcgccagcgc
     7801 ggtgtagtag ctctcgaagc cgttgtccgc cccgttgccc atcagccgga tgatcttatt
     7861 gatgactgcc atgatttgat tcctttcagc gccgccaata accagcgcgg cgcccgcatc
     7921 gacctaatga t
//
LOCUS       DASUBI010000330         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_369951, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000330 DASUBI010000000
VERSION     DASUBI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369951"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFU72_06390"
     CDS             complement(<1..1269)
                     /locus_tag="VFU72_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEU5423150.1"
                     /translation="MTSPISIAPDTSAARRERQVLSPPRTHVLPRQITLETLFYAVIF
                     LLALVTRFWDLGSRALHHDESLHAYFSWIFAEGFGYRHDPLMHGPFLFHANALIYLLL
                     GASDATSRIVPALAGVVVVMLPWLLRDERFLGRWGALTASALLLISPSILYYSRFLRH
                     DIYCLLGTFALFIAIVRYVDEQQPKWAVLGGIAIGFLLCTKEVSFIVLFIFVAFLGAA
                     LIYRVAPILYAVVAVALVAFAVLAKALKALGAAPLPGIPWSNPTGPQIRTFTVHLLTH
                     PLVVGALGITLLALVATLWLLDRRRDPERGWLDGLLGDAPDDSVAGALRELLWERRGL
                     LWGIAFAVGIFVLFYSTLFTNREGLASGTFGAVGYWLGQQGVQRGEEPWFYYLLLLPQ
                     YEFVAVVLFPILGGWFAWTLLQARRAGAPAT"
     gene            complement(1435..1758)
                     /locus_tag="VFU72_06395"
     CDS             complement(1435..1758)
                     /locus_tag="VFU72_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423151.1"
                     /translation="MASQEQPTAEVMEQLRPHVTSVARRINVDEFTTVQFVEALHLDD
                     PARAAYEEAVRRWPENEHLAKMILHGQVIPQLLRESGLVEWAGFAYGEEDPYAVPAWW
                     RRLTP"
     gene            complement(1778..2599)
                     /locus_tag="VFU72_06400"
     CDS             complement(1778..2599)
                     /locus_tag="VFU72_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU5423152.1"
                     /translation="MGALIVIGILVVLALIIFSATVKVVQEYERGVVFRLGRLAGVRG
                     PGLILLVPYIERMVKVDLRTLTMDVPAQEVITKDNVTIRVNAVAYFRVVDPATAVVNI
                     ADYVRATSQIAQTTIRSALGQAELDELLSEREQINQRLQTIIDEQTEPWGVKVSVVEI
                     KDVELPETMQRAMARQAEAEREKRAKIIHAEGEAAASQQLAQAANVITASPGALELRY
                     LQTLSEIATERNSTIVFPIPVDVISRFLGDMTGHHDGAHAGNGVKPATVEPPVHD"
     gene            complement(2612..>3119)
                     /locus_tag="VFU72_06405"
     CDS             complement(2612..>3119)
                     /locus_tag="VFU72_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU5423153.1"
                     /translation="DNAWWAIGVLLAAEMATLASAHWVRHALLTAVGVAGFAIGSFLL
                     LAIASIPALVVLASVAVVAVASFALAAAARRAHDWPVQTGREVLIGRLAETREQLDPQ
                     GFVLINGELWRATSVAGPIPRGATVRVLAIEGLTLLVTLDGRGQVEDLRDADRREAAT
                     TRLERFGG"
BASE COUNT          548 a         1036 c         1095 g          440 t
ORIGIN      
        1 cgtggcgggc gcgccggcgc gccgggcctg aagcagcgtc caggcgaacc agccgccgag
       61 gatcgggaag agaacgacgg ccacgaactc atactgcggc agaaggagca ggtagtagaa
      121 ccagggctcc tcaccgcgct gcacaccctg ctgccccagc cagtagccga ccgcgccgaa
      181 ggtgccgctg gccaggccct ccctgttggt gaagagggtg gagtagaaga gcacgaagat
      241 gccgacggcg aaggcgatgc cccagaggag cccgcgccgc tcccagagca actcccgcag
      301 cgctccggcg acggagtcgt ccggcgcgtc acccagcagt ccgtcgagcc agccgcgctc
      361 aggatcgcga cggcggtcga gcagccagag cgtggccacc agcgccagca gggtgatgcc
      421 gagggctccg acgaccaatg ggtgggtcag gaggtgcacg gtgaaggtgc ggatctgtgg
      481 cccggtcggg ttggaccagg ggataccggg aagtggcgcc gcgcccagcg ccttgagcgc
      541 cttggcgagc acggcgaagg cgaccagcgc gacggcgacg acggcgtaga ggatcggcgc
      601 cacgcggtag atcaacgccg cgccgagaaa ggccacgaag atgaacagga caatgaagct
      661 gacttccttg gtgcagagga ggaagccaat ggcgatgccg ccgagcacgg cccacttggg
      721 ctgttgctca tcgacataac ggacgatggc gatgaagagg gcgaacgtgc cgagcaggca
      781 gtagatgtcg tgccgcagga agcggctgta gtagaggatc gagggtgaga tcaggagcag
      841 cgccgaggcc gtgagcgcgc cccagcggcc gaggaagcgc tcgtcgcgca gcagccaggg
      901 gagcatgacc acgaccacgc cggccagcgc cggcacgatg cgcgaggtgg catcgctggc
      961 gcctagcagc agatagatca acgcgttggc gtgaaagaga aagggcccgt gcatcagtgg
     1021 gtcgtgacgg tagccgaagc cctcggcgaa tatccaggag aagtaggcgt gcagcgactc
     1081 gtcgtgatgc agcgcccgcg agccgaggtc ccagaagcga gtgacgagcg cgagcaggaa
     1141 gatgacggca tagaagaggg tttcgagcgt gatctgcctg ggcagcacgt gcgtgcgcgg
     1201 agggctgagg acttgccgct cacgcctcgc ggcggacgtg tccggggcga tcgagatggg
     1261 agaggtcacc tgctccacgg ggaggaagat ccgatccggc cgatgcggcg aatgcggcaa
     1321 gaatggccaa acgtcgggcg cgattctaca ccgagtgcct gaggatctgc cacgtagcgg
     1381 atggtagggg gctcgtgccc accgtgcgcc acccaacgtc gtcgaccgcc gccgctacgg
     1441 cgtcagcctg cgccaccaag cggggacggc gtaggggtct tcctcgccgt aggcgaagcc
     1501 ggcccactcg accagcccgc tctcgcgcag aagctgcggg atcacctgcc cgtggaggat
     1561 catcttggcc agatgctcgt tctccggcca gcggcgcacg gcctcctcgt aggcagcccg
     1621 cgcgggatcg tcgagatgga gcgcttccac gaattgcacc gtggtgaact cgtccacgtt
     1681 gatgcgccgc gccaccgagg tcacgtgcgg ccgtagctgc tccatgacct cggcggtagg
     1741 ttgctcctgg ctggccacgc ggtgctcgcg ttcctagtta gtcgtgcacc ggtggctcga
     1801 ccgtggccgg cttgacgccg ttgcccgcgt gggcgccgtc gtgatgcccg gtcatgtcgc
     1861 cgaggaagcg gctgatcaca tccaccggaa tcgggaagac gatggtgctg ttgcgctcgg
     1921 tcgcgatctc gctgagcgtc tgcaggtagc gcagctccaa cgcgcccggc gaggccgtga
     1981 tgacattggc ggcctgcgcc aattgctgcg aggccgcggc ctcgccctcg gcgtggatga
     2041 tcttggcccg cttctcgcgc tcggcctccg cctggcgcgc catggcgcgc tgcatcgtct
     2101 ccggcagctc gacgtctttg atctcgacca cgctgacctt gacgccccac ggctcggtct
     2161 gctcgtcgat gatcgtctgc aggcgctggt tgatctgctc ccgctcggag agcagctcgt
     2221 ccagctcggc ctgccctagc gcgctgcgga tcgtggtctg cgcgatctgc gaggtggcgc
     2281 gcacgtagtc ggcgatgttg accacggccg tggccggatc gaccacgcgg aagtaggcca
     2341 ccgcgttgac gcggatggtg acgttatcct tggtgatgac ttcctgggcc ggcacgtcca
     2401 tcgtcagtgt gcgcaggtcg accttgacca tgcgctcgat gtaaggcacc agcaggatca
     2461 ggcccggccc gcgtaccccg gccagccggc cgagacggaa gacgacgccg cgctcgtact
     2521 cctgcaccac tttgacggtg gcgctgaaga tgatgagcgc caggacgacc agaatgccga
     2581 tgacgatcaa tgcacccaac tgaaactcct ctcagccccc gaagcgctct agccgcgtcg
     2641 tggccgcctc ccggcggtcc gcatctcgca ggtcttccac ctgccctcgc ccgtcgagcg
     2701 tgacgagcag ggtcagaccc tcgattgcga gcacacgcac cgtcgcgccg cgcggaatcg
     2761 gccccgcgac cgaggtcgcc cgccagagtt cgccgttgat cagcacgaag ccctgcggat
     2821 cgagctgctc ccgcgtctcg gccagccggc cgatcagcac ctcccgtccc gtctgcaccg
     2881 gccagtcgtg cgcgcgacgc gcggccgcgg ccagcgcgaa cgaggccacg gccacgaccg
     2941 cgacgctcgc cagcacgacg agcgcgggga tcgaggcgat tgccagcaac aggaacgagc
     3001 cgatcgcgaa gccggccacg cccaccgccg tcagcagcgc gtggcgcacc caatgcgccg
     3061 aggccagggt ggccatctcg gccgccagca gcacgccgat ggcccaccag gcgttatcg
//
LOCUS       DASUBI010000331         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_794120, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000331 DASUBI010000000
VERSION     DASUBI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794120"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFU72_06410"
     CDS             complement(<1..727)
                     /locus_tag="VFU72_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423154.1"
                     /translation="MALAGGRRPIMIMALACTALAVLAACSSDGSAARPVLRPVLPAG
                     ATAVLGKGTLYLLLGNEAISANLWRVDLGSGLVRQLTFNPPQYGVSNFSASTAGLVLG
                     NARSGVDEADIMVAGKPQPLSGGVGDSPQINSAGQIVDVAFAEQGVRHGPWSRDRLLY
                     WANPAARYRTIYQAKPSSLVDLGWNPTGTMILAAEGPDAYSRLFAVNARGQIVSQFVT
                     VPGFPHGWAWGRYGLAVGYQSAAP"
     gene            complement(745..1122)
                     /locus_tag="VFU72_06415"
     CDS             complement(745..1122)
                     /locus_tag="VFU72_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423155.1"
                     /translation="MMIGSKWGTKVVNGAAMATLNFMLGKGAASIGGSTQVKNYATHS
                     GDTGGDPNLAVPKAWRKYSIIRVNAFYISSHDLFFQGTGSSEDNVGHALYEFDTKGTV
                     DFKYGAAIQVRAFCAEVSCPAGF"
     gene            complement(1471..2142)
                     /locus_tag="VFU72_06420"
     CDS             complement(1471..2142)
                     /locus_tag="VFU72_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423156.1"
                     /translation="MIRVALADDQALVRSGFAALLDAEDDIEVVGEAADGDAAVQLAR
                     RHRPDVLLMDIRMPVLDGIAATRTIAASQELADVHVVILTTFELDEYIFDGLRAGAAG
                     FLVKDTDAAELLRAVRVVAAGEALLSPAVTRRLIAEFTARTAGTTQVPGVDELTARER
                     EVVSLIATGLSNEEIAGRIYVSQSTVKTHVARAMMKLRARDRAQLVVFAYQSEIVRPG
                     ANGYI"
     gene            complement(2139..>2325)
                     /locus_tag="VFU72_06425"
     CDS             complement(2139..>2325)
                     /locus_tag="VFU72_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423157.1"
                     /translation="VPATVAARPAAARPPERDPGTGSGIAGMAERAQALGGTLQTARR
                     PDGGFGVLATLPIEGSR"
BASE COUNT          396 a          810 c          715 g          404 t
ORIGIN      
        1 tgggcgccgc gctctgatag ccgaccgcca ggccatacct tccccaggcc cacccgtgag
       61 ggaagccagg cacggtcacg aactggctca caatctgccc tcgcgcattc acggcgaaca
      121 atcggctgta ggcgtccggc ccttctgccg ccaggatcat cgtgccagta gggttccagc
      181 ctaggtcgac caaacttgac ggttttgcct ggtaaatggt ccggtaccgg gccgccggat
      241 tggcccagta aagcagccgg tcgcgcgacc acgggccatg ccggacacct tgctcggcaa
      301 atgcgacgtc cacgatctgc cccgccgaat taatttgagg cgagtcgccg acaccaccgc
      361 tgagcggctg aggcttgccc gcgaccatga tgtcggcctc atccacacca gaccgggcat
      421 tcccaagcac cagcccggcc gtcgacgcgc tgaaattcga caccccatac tgcggcgggt
      481 tgaacgtcag ctgccgaacc agcccgctgc cgagatctac ccgccacagg ttcgccgaga
      541 tggcctcgtt gcccagcagc aggtacaacg tgcctttgcc caatactgcc gtcgcgcccg
      601 ctggcagcac cggccggagt accggtcgtg ctgccgagcc gtccgacgag cacgcagcca
      661 gcaccgccag cgccgtgcag gcgagcgcca tgatcatgat cggtcgacgg ccgcctgcta
      721 gcgccacctc gcatcctccc ttgctcagaa gcctgccggg cacgaaacct cggcgcagaa
      781 agcccgaact tgaatggccg cgccgtactt gaagtccacc gttcccttgg tgtcgaattc
      841 gtacaaggcg tgaccgacgt tgtcttcgct gcttccggtg ccctggaaga agagatcgtg
      901 cgaggagatg tagaacgcgt tcacccggat gatgctgtac ttgcgccatg ccttcggcac
      961 ggccaggttg ggatccccac cggtgtcccc ggagtgcgtg gcgtaattct ttacctgcgt
     1021 gctcccgccg atcgaagccg cacccttgcc caacatgaaa ttcaacgtcg ccattgccgc
     1081 gccattaacg accttggtgc cccatttact gccgatcatc atcggccgag catatttgag
     1141 tacgatcgct ccggcgcacg ccaccgctca ccgcgtttgc ggtagccgag aaactggcgc
     1201 tggccgagac agttttggga tgaccggcag gtcgttgcgg gcccgacgcc attgccggac
     1261 cgaccccgca ggtcatcccg gctatgagag gggtgccaga taaggacaat aatgcccgct
     1321 tccttaaagt attccccgtc atgctcgttc ccctctgcga gcaccgtagt gcccttagtt
     1381 gcgatcagga acgtatcacg gtcgccggta tacgacaatg gtcagatagc ccaatggccc
     1441 gaaggtgaca ttaccttaaa tgagtagcag ttagatataa ccgttcgcgc cgggtcgaac
     1501 tatttcggat tgataggcga agaccacgag ctgggcccgg tcgcgcgccc tgagcttcat
     1561 catggcccgg gcgacgtgag tcttgaccgt ggactgactc acgtagatcc gcccggcgat
     1621 ctcctcgttc gacaggccgg tcgcgatcag cgagactacc tcgcgctccc gcgcggtcag
     1681 ctcatcgaca cctgggactt gggtcgtgcc cgccgtccgg gcggtgaact cggcgatcag
     1741 tcgcctggtc acggccggcg acagcagcgc ctcgccggcc gcgacgactc tgaccgcgcg
     1801 cagcagctcg gccgcgtcgg tgtccttgac caggaacccg gccgcgccgg cccgcagccc
     1861 gtcgaagatg tactcgtcca gctcgaacgt ggtcaagatg accacgtgca cgtcggccag
     1921 ctcctggctc gcggcgatcg tcctggtcgc ggcgatgcca tcgaggaccg gcatcctgat
     1981 gtccatcagc agcacgtccg gccggtgccg gcgtgcgagc tggacggccg cgtcgccgtc
     2041 ggccgcttcc ccgaccacct cgatgtcgtc ttcggcgtcg agcagcgcgg cgaagccgga
     2101 gcggaccagc gcctggtcgt cggcgagcgc gaccctgatc atcggcttcc ctcgatcggc
     2161 agcgtcgcca gcacgccgaa gccgccgtcc ggtcgccgag cggtctggag ggtgccgccg
     2221 agggcctggg cgcgttcggc catcccggcg attccgctgc cggtgcctgg atcgcgctct
     2281 ggcggccttg ctgccgcggg ccgggcggcg accgtggccg gaacc
//
LOCUS       DASUBI010000332         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_997499, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000332 DASUBI010000000
VERSION     DASUBI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997499"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..2032)
                     /locus_tag="VFU72_06430"
     CDS             complement(89..2032)
                     /locus_tag="VFU72_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEU5423158.1"
                     /translation="MDLSVRTQLAHPLTPLSVEEIEQALGILRAARPFEAGTRVASVA
                     LREPPKAAVLAYPGEPVEREAAVLLLDKPGGAVHEAVVSLSRGGGGGELRAWRDLPGV
                     QPPIMGEEFASCEAAIKADPRVLEALARRGVTDLDLVMVDPWSAGYYGGAHDDPERGR
                     RLVRALCWVRPRPGGHGYAHPIDNLAILCDLNTLEVIEIEDFGVVPVPQTPGEYAGDS
                     LGPPRADLKPLEIHQPEGPSFQVDGYEVRWQKWHFRVGFTPREGLVLHTLGYQDGGRE
                     RPILYRASLAEMVVPYGDPHPAQWRKNAFDAGEYAIGTLVNSLELGCDCLGEIHYFDA
                     ALADGDGRAQTVRNAICLHEEDAGILWKHLDFRTGATEVRRSRRLVISWIATVGNYEY
                     GFYWYLYQDGTITFEIKLTGIVSTGALPPGERTPFGQLLSPDGLYGPIHQHFFSLRLD
                     LDIDGPLNSVYEIHTESVPLGPETPHGNAYRTYSELLESEQGAQRVIDPLAARYWKVV
                     NPNSLNAVGEPVGYRLLPHSNVLPFAQPSAAVMARAGFMAKHLWVTPYAPDELYAAGE
                     YPNQHPGGAGLPAWTAADRSVAETDVVLWYTLGSHHPVRLEDWPVMPVQTVGFTLQPQ
                     GFFDANPALDVPPSPGHGQNGHC"
BASE COUNT          362 a          823 c          866 g          352 t
ORIGIN      
        1 actgggagaa ggggagttaa gctacgggcg acacgtgcgt gctcccctct cccagcattg
       61 ggagaggggc ggggggtgag ggcccgttct agcaatgccc gttctgaccg tgccccggac
      121 tcggcggcac gtcgagcgcc gggttggcgt cgaagaagcc ctgcggctgc agcgtgaagc
      181 cgaccgtctg caccggcatc accggccagt cctccagccg caccgggtgg tgcgagccca
      241 gcgtgtacca gagcaccacg tcggtctcgg ctaccgaacg atcggccgcc gtccacgccg
      301 gcagcccggc gccgccgggg tgctggttgg ggtactcgcc cgcggcatag agctcgtccg
      361 gcgcgtaggg cgtcacccac aggtgcttgg ccatgaagcc ggcgcgcgcc atcaccgccg
      421 cgctgggctg ggcgaagggg agtacgttgg agtgcggcag cagccggtag ccgaccggct
      481 cgccgacggc gttcagcgaa ttcgggttga ccaccttcca gtagcgcgcg gccagcgggt
      541 cgatcacccg ttgcgccccc tgctcgctct cgagcagttc ggagtaggtg cggtaggcgt
      601 tgccgtgcgg ggtctccggc ccgagcggga cggattcggt gtggatctcg tagaccgagt
      661 tgagcgggcc gtcgatgtcc aggtcgagcc gcaggctgaa gaagtgctgg tggatggggc
      721 cgtagaggcc gtcaggcgag aggagctggc cgaagggcgt gcgctcgccc ggcggcagcg
      781 cgccggtcga gacgatgccg gtcagcttga tctcgaaggt gatggtgccg tcctggtaga
      841 gataccagta gaagccgtat tcgtagttgc cgacggtggc gatccaggag atcaccaggc
      901 ggcgcgagcg gcgcacttcg gtcgcgccgg tgcggaaatc caggtgcttc cagaggatgc
      961 cggcgtcctc ctcgtgcagg cagatggcgt tgcgcacggt ctgcgcccgg ccgtcgccat
     1021 ccgccagcgc cgcgtcgaag tagtggatct cgcccaggca gtcgcagccc agctccagcg
     1081 agttgaccag cgtgccgatg gcgtactcgc cggcgtcgaa ggcgttcttg cgccactgcg
     1141 ccgggtgagg atcgccgtag ggcaccacca tctcggcgag cgaggcgcgg tagaggatgg
     1201 gccgctcgcg cccgccgtcc tggtagccga gggtgtgcag caccagcccc tcgcgcgggg
     1261 tgaagccgac gcggaagtgc cacttctgcc agcgcacctc gtagccgtcg acctggaagc
     1321 tcggcccctc gggctggtgg atctccagcg gcttgaggtc ggcgcgcggc gggccgagcg
     1381 agtcgccggc gtactcgccg ggggtctgcg gcaccggcac cacgccgaag tcctcgatct
     1441 cgatcacctc cagcgtgttg aggtcgcaga ggatggcgag gttgtcgatc gggtgggcgt
     1501 agccgtggcc gccgggccgg ggccgcaccc agcagagcgc ccgcaccagc ctccgcccgc
     1561 gctcggggtc atcgtgggcg ccgccgtagt agccggcgga ccagggatcg accatcacca
     1621 gatcgagatc cgtgacgcca cgacgggcca gcgcctcgag cacgcgcggg tcggccttga
     1681 tcgccgcctc gcagctggcg aactcctcgc ccatgatcgg cggctgcacc cccggcaggt
     1741 cgcgccacgc gcgcagctcg cccccacccc caccccggct gagcgagacg acggcctcgt
     1801 ggaccgcgcc gcccggcttg tccagcagca gcacggccgc ctcgcgctcg accggctcac
     1861 ccgggtaggc cagcaccgcc gccttgggtg gctcgcgcag cgcgaccgag gcgacgcgcg
     1921 tgccggcctc gaacggccgc gcggcgcgca ggatgcccag cgcctgctcg atctcctcga
     1981 cgctgagtgg agttaaggga tgcgccagct gggtgcgcac gctcaggtcc atgatcgaca
     2041 cctcctgcgt ctcgctcctg gtggaagccg gtgtgccgct ggttcgcggg gggaagactg
     2101 gccctactct acggagcagc catgaagcgt gtcaagcgcc gcgcccggca tacgaattgc
     2161 acatttggga taggcgagcc cccgcaccgg agactcgcgc cgcactcgat cgccagcaca
     2221 ccagggaggc cgccatggat gccaagacgc tcaagggcat cgccgtcatc gcgattcaag
     2281 atggacaaaa ggtcgggacc gtcagccaga tccgcttcaa cctcgaccgt cgccggattc
     2341 agggcttcga ggtcggcacc ggcggcctct tcggcggcaa caccctcttc ctgccgatgg
     2401 aca
//
LOCUS       DASUBI010000333         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_659522, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000333 DASUBI010000000
VERSION     DASUBI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659522"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFU72_06435"
     CDS             <1..501
                     /locus_tag="VFU72_06435"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5423159.1"
                     /translation="VVALCRYYHFPTLQVIDAIAPGLLMAQAFGRIGCIINGDAWGAP
                     TASRFAFIYTNPKALLPPDLLGVPTYPYPLYDLVINIAIFAVIWRLRRRGLPNGALFA
                     IFTALYGASRFLISFMRQERVWFWDLQEAQVVAILALIASLAALALLFRHRAPRDVNV
                     DVPASV"
     gene            694..769
                     /locus_tag="VFU72_06440"
     tRNA            694..769
                     /locus_tag="VFU72_06440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:727..729,aa:Ala,seq:cgc)
     gene            908..1180
                     /locus_tag="VFU72_06445"
     CDS             908..1180
                     /locus_tag="VFU72_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423160.1"
                     /translation="MRGGKYRLIGRRADRPQSLVFRDQQGRHFLRTNCGARLVRLTAR
                     DAQRLLRQYPYTSVLDGAWRDYQEALGYECPLPIPDAPGILPADGN"
     gene            complement(1466..>3240)
                     /gene="ftsH"
                     /locus_tag="VFU72_06450"
     CDS             complement(1466..>3240)
                     /gene="ftsH"
                     /locus_tag="VFU72_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5423161.1"
                     /translation="QVLVKYKAGHQPLTAITNKEAGSLTDTLTALGVTKDQLAAVNIS
                     VEPASSWGSWLGTLGFILPTLFLIGVFVFMMRQAQGTNNQALSFGKSRARLFMGNKPT
                     ITFSDVAGVEEAKQELAEVVEFLKYPDKFAALGARIPRGVLLVGPPGTGKTLLARAVA
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFVDEIDAVGRQRGAGL
                     GGSHDEREQTLNQILVEMDGFDSTTNVIVIAATNRPDVLDPALLRPGRFDRQVVLDRP
                     DIAGRRAILDVHARGKPFESEVDLEVLARQTPGFSGADLENLVNEAAILAARRNKKTV
                     GLNELTEAIDRVIAGPERKSRVISERERLMTAYHEAGHALVARMLPNADPVHKVSIVA
                     RGMMGGYTRVLPEEDRFFWTKKQFEDQLAVFMGGHVAEELIFQEISTGAANDIERMTN
                     LARQMVTQYGMSKRLGPLAFGRKEELVFLGREISEQRNYSEEIAFEIDKEIRELIDDA
                     YGRAKQILTDNMDKLEAIAMLLMDRETIDGAELEALFDEPRPRPQLFGPAAQRPAVAS
                     EAQPSKEYDQRPSPAPRMRPQPAS"
BASE COUNT          477 a         1143 c         1045 g          575 t
ORIGIN      
        1 gtcgtcgcgc tctgccgcta ttaccacttc cccaccctgc aggtgatcga cgccatcgcg
       61 cccgggctgt tgatggcgca ggccttcggc cgcatcggct gcatcatcaa cggcgacgcc
      121 tggggcgcgc cgaccgcgtc acgcttcgcc ttcatctaca ccaaccccaa ggctctgctg
      181 ccgcccgatc tgctgggcgt gccgacctac ccctacccgc tctacgatct ggtgatcaat
      241 atcgcgatct tcgcggtcat ctggcgcctg cggcggcgtg gtctgcccaa cggcgcgctc
      301 ttcgccatct tcaccgcgct ctacggcgcc tcgcgcttcc tgatcagctt catgcgccag
      361 gagcgagtct ggttctggga tctgcaagag gcgcaagtgg tggcgatcct ggcgctgatc
      421 gcatcgctgg ccgcgctggc acttctcttc cggcatcgcg cgccgcgcga cgtgaacgta
      481 gacgtacccg cctcggtcta gccccctccg gcgcgggggc agagcggcta gctgtcgtca
      541 tgctgccggc acgccggcag gcaggagacc gtaagacccg cactccaggc gcctcgcctc
      601 aacgcggttg acacccccgg gccaccgtcg gtatacttct cctcgtctgc ccggccggta
      661 gattaatccc tcgtgtcgga cagcaacacc cgtgggggca tagctccgct gggagagcgc
      721 tacaatcgca ctgtagaggt cgggggttcg aatccccctg cctccaccac gcgggttcgt
      781 tcctcggtcc cggtcgtact cgtcagtgac acgcggatac ctctgggagc cggttcggtc
      841 cccgtcgcat cgtccatcca cgtgcatgcg ggtcttggcc cgcccctgag tgaaagggga
      901 accgtccatg cgtggtggca agtaccgtct tatcggtcgg cgcgcggacc ggccacagag
      961 cctcgtcttc cgcgatcagc agggccggca ttttctgcgc accaactgcg gcgcgcgcct
     1021 ggttcggctc acggcgcgcg atgcccagcg cctgctgcgt cagtacccct atacctccgt
     1081 gctcgacggc gcctggcgcg actatcagga agcgctcggc tacgagtgcc cgctgccgat
     1141 tccggacgca ccggggatcc tcccggccga cggcaactga gggcgggatt gcagccttcc
     1201 gtgtcgacat cgtgccgagc ggcgcgcggg actaacgtcg ccgcgctcac aaacgaagcc
     1261 tgctacaagc aggctgagag catggctgga ccttagccgg cttcgacccg agcgcggcgg
     1321 ctccagcccc gcgcgacgta gcccggtcgc cggaagtatt cgtcagactc caccacccgc
     1381 cgtaccgagg gcactcctga tctgacgacg aataaacggc cccggcgttt tgccggggcc
     1441 gtggtcgtgt cgaaggtcaa ccgcgctacg acgcgggttg gggccgcatg cgcggggccg
     1501 ggctggggcg ctggtcgtat tctttgctcg gctgcgcctc gctggccacc gccggacgct
     1561 gcgccgccgg accgaagagc tgcggccggg ggcgcggctc gtcgaagagc gcctccagct
     1621 cggcgccgtc gatcgtctcg cggtccatca agagcatcgc gatcgcctca agcttgtcca
     1681 tgttgtcggt cagaatctgc ttggcgcgcc cgtaggcgtc gtcgatcagc tcgcggatct
     1741 ccttgtcgat ctcgaaggcg atctcttcgc tgtagttgcg ctgctcgctg atctcgcgcc
     1801 cgaggaaaac cagctcctcc ttgcggccga aggccagcgg gccgagccgc ttgctcatgc
     1861 cgtactgggt caccatctgg cgcgccaggt tggtcatgcg ctcgatatcg ttggcggcgc
     1921 cggtcgagat ctcctgaaag atcaactcct cggccacgtg gccgcccatg aagaccgcca
     1981 gctgatcctc gaactgcttc ttggtccaga agaagcggtc ctcctccggc aggacgcggg
     2041 tgtagccgcc catcatgccg cgcgccacga tcgagacctt gtggaccgga tcggcattgg
     2101 gcagcatgcg cgccaccagc gcgtgcccgg cctcgtggta ggcggtcatc agccgctcgc
     2161 gctcgctgat cacgcggctc ttgcgctcgg ggccggcgat gacgcggtcg atcgcctcgg
     2221 tcagctcgtt gagaccgacc gtcttcttgt tgcgccgcgc ggccagaatg gcggcctcgt
     2281 tgaccaaatt ctccaggtcg gcgccggaga agccgggcgt ctgccgcgcc agcacctcca
     2341 ggtcgacttc gctctcgaac ggcttgccac gcgcgtgcac gtcgaggatc gcccgccgcc
     2401 ccgcgatgtc cggccggtcg agcacgacct ggcggtcgaa gcggccgggg cgcagcagcg
     2461 ccgggtcgag cacgtcgggc cggttggtgg ccgcgatcac gatcacgttg gtggtgctgt
     2521 cgaagccgtc catctcgacc aggatctggt tcagcgtctg ctcgcgctcg tcgtggctgc
     2581 cgcccagtcc agcgccgcgc tggcgcccga cggcgtcgat ctcgtccacg aagacgatgc
     2641 agggcgcgtt gcgcttggcc tggtcgaaca ggtcgcgcac gcggctggcg ccgacgccga
     2701 cgaacatctc gacaaactcc gagccgctga tggagaagaa cggcaccccg gcctcgccgg
     2761 ccacggcgcg ggccagcagc gtcttaccgg tgcccggagg gccgaccagc aagacaccgc
     2821 gcgggatacg cgccccgagc gcggcgaact tgtccgggta tttgaggaac tcgaccacct
     2881 cggccagttc ctgcttggcc tcctccacgc ccgccacgtc gctgaaggta atggtcggct
     2941 tattgcccat gaacaggcgt gcccggctct taccgaacga cagggcctgg ttattcgtgc
     3001 cctgggcctg gcgcatcatg aagacgaaca cgccgatcag gaacagcgtc ggcaggataa
     3061 agccgagcgt ccccagccac gatccccagg aggatgccgg ctccacgctg atgttcaccg
     3121 cggccagttg gtccttggtg acgccgaggg cggtcagcgt atcggtcagg ctacccgcct
     3181 ctttgttggt gatcgcggtc agcggctgat ggcccgcctt gtacttgacc agaacctggt
//
LOCUS       DASUBI010000334         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1064083, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000334 DASUBI010000000
VERSION     DASUBI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064083"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFU72_06455"
     CDS             <1..212
                     /locus_tag="VFU72_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU5423162.1"
                     /translation="VLDKDGKTRALMVSDPFHMFRVDHMARDLGLQPLSSPTQTSPIR
                     PGSATEQRYAVREVFAYLAYLFLQR"
     gene            complement(203..1345)
                     /locus_tag="VFU72_06460"
     CDS             complement(203..1345)
                     /locus_tag="VFU72_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5423163.1"
                     /translation="MQFEPFALERWMTAWETEVEYDLAESGIKPLTVRELLALASGEP
                     ALERLLDISLGYSEARGTEELRTILAGLYRDTGPDNVLVTTGAIEANFLLFSVLIRPG
                     DHVVAVHPAYQQLQSVPRGLGAEVDLWRLRPENDFRYQLDELERLVSPHTRLIVINSP
                     HNPTGAILTQAELERIYALAERVDAYVLSDEAYRWLELPGGEPFAGPARDLGPRGLSV
                     GTLSKPLGLPGLRIGWLAAPEELVAACWGQRDYISLSPAKMSDYLATLALRHRTQIFT
                     RTRGIVERNLATLNQWLREREGVVDWVPPRGGLLGLLHYDIAVPSAELADRLAREASV
                     MLAPGSAFGFEHHLRIGLGADPAVFAEGLARATPVIEAALAERGQR"
     gene            complement(1565..4039)
                     /gene="leuS"
                     /locus_tag="VFU72_06465"
     CDS             complement(1565..4039)
                     /gene="leuS"
                     /locus_tag="VFU72_06465"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5423164.1"
                     /translation="MATTERVVRPERYDPNAIEDKWQRIWEETGLYQTVEDDSRPKWY
                     HVTMYPYPSGDLHIGHWYAMTPSDAGARYKRMQGYNVLFPMGFDAFGLPAENAAIKRG
                     IHPYTWTMTSIPRMEQQMRSMGTMIDWSREVITCVPEYYKWNQWFFLEFYKRGLAYRA
                     MAPVWWCPTCQTSLANEQVINGRCERCESEVYRRDLEQWFFRITKYADELLSFAGIEW
                     PERVRTMQRNWIGRSEGARVRFPTAIGEPIEIFTTRPDTLWGATFMVLAPEHPLVEKL
                     TTPERRAAVAAYVDAARRETEIERTSTDKEKTGEFIGAYATNPVNGEAVPIWIADYVL
                     MGYGTGAIMAVPAHDQRDFEFARKFGLPIEVVIEPEGETLDAVEINEAYAGEGVMVHS
                     GPFNGTPTAGGQAIDRVIAWLKEAGIGQGEVTYRLRDWLISRQRYWGTPIPIVYCDTC
                     GVVPVPEDQLPVILPQDAEFTPTGQSPLVTDEEFVSTTCPRCGGPARRETDTMDTFVD
                     SSWYQYRYLSPHDDTAPFTKEKADYWMPVDRYTGGIEHAILHLMYARFFTKAMRDMGL
                     VDYGEPFVRLANQGIILGENSEKMSKSRGNVVDPDDLVSQLGADVVRMYLMFIGPWDQ
                     GGPWNSRGIAGVERFVRRVWSLVQETADNPAPEAATTEAHELRRITHRTIQAATEDIE
                     NFRFNTMIARLMELQNELAPRKDTDVASTRAWREALEALTLMLAPIAPYLAEELWQRL
                     GQTYSVHQQLWPTFDAELVKEELIELPVQVNGKVRDHLDVPADISQENAIEQAMASPR
                     VAEFTAGKQVQRVVYVPRRLINIVAR"
     gene            complement(4186..4261)
                     /locus_tag="VFU72_06470"
     tRNA            complement(4186..4261)
                     /locus_tag="VFU72_06470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4226..4228),aa:Ala,seq:ggc)
     gene            complement(4568..>5662)
                     /locus_tag="VFU72_06475"
     CDS             complement(4568..>5662)
                     /locus_tag="VFU72_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423165.1"
                     /translation="YGGNGRVGVGVDYATSVHLTDDTVLDDVTGASSIAIIDFSQATH
                     DWVGSQQTLSARDVLTQIMPPGPYSYNVVGRRLSVAGFAQPAIAPTAWDAGVLRAPGP
                     GTLLLGGDLLAGGSGLSSFVSQARATGSHRVVLVVAGFFSTGDASSTANTYAQALKKL
                     GWSQSKDQITILNFEDTTGWSRVAPDVVDGAAGVILVGGDQSQMARPLADATFRALVE
                     QAIDTAPVVLTDRAMTPAMGEQYVTNADPTTQNLQSQSIAAFSSDYAEVEPGLGYVHA
                     AFEPRLTTDQRWGRLYALAQADPQTLVFGISEQTALSLDGGTATVVGERSVVALDGRT
                     ATFATGDNGAIAAFNVWLNLYAPGETVAGP"
BASE COUNT          863 a         1955 c         1884 g          960 t
ORIGIN      
        1 acgtgttgga caaagacggc aagacacgcg cgctgatggt cagcgacccc ttccacatgt
       61 tccgggtgga tcacatggcg cgcgatctgg gtctccagcc gctgtcgtcg ccgacccaga
      121 ccagcccgat tcgtccgggc agcgccaccg agcaacggta tgccgtgcgc gaggtcttcg
      181 cctacctggc ctatctcttc cttcagcgct gaccgcgctc ggccagcgcc gcctcgatga
      241 ccggcgtggc gcgcgccagc ccctcggcga agacggccgg atccgcgccc agtccgatgc
      301 gcaggtgatg ctcgaagccg aaggccgagc cgggcgccag catcacgctc gcttcgcgtg
      361 ctagccggtc ggccaactcg gcggacggca cggcgatgtc gtagtgcagc agcccgagga
      421 gtccgccgcg cggcggcacc cagtcgacga ccccctcgcg ctcgcgtagc cattggttca
      481 gcgtcgccag attccgctcc acgatgcccc gcgtgcgcgt gaagatctgc gtgcggtgcc
      541 gcagcgccag cgtcgccagg tagtcgctca tcttggccgg gctgagcgag atgtagtcgc
      601 gctggcccca gcaggccgcg accagctcct cgggcgccgc cagccagccg atgcgcagcc
      661 ccggcagccc gagcggtttc gagagcgtgc cgacgctcaa cccgcgcggg ccgaggtcgc
      721 gcgccggccc ggcgaagggc tcgccgccgg gcagttccag ccagcgatag gcctcgtcgc
      781 ttagcacgta ggcatcgact cgctcggcca gggcgtagat acgctccagc tcggcctgcg
      841 tcaggatggc gccggtcggg ttgtgcggcg agttgatcac gatcagccgg gtgtgcggcg
      901 agacgagccg ctccagctcg tccaactggt agcgaaagtc gttctccggg cgcagccgcc
      961 agagatcgac ctcggcgccc agtccacgcg gtacgctctg cagctgctgg taggcggggt
     1021 gaaccgccac cacgtggtcg ccggggcgaa tcagcacact gaagaggagg aagttggctt
     1081 cgatcgcgcc ggtggtcacc agcacgttgt ccggcccggt gtcgcgatac aggcccgcga
     1141 ggatcgtgcg cagctcctcg gtaccgcgcg cctcgctgta gccgagcgag atgtcgagca
     1201 gccgctccaa cgccggttcg ccgctggcca gcgccagcag ctcgcgcacc gtcagcggct
     1261 tgatcccgct ctcggccagg tcgtactcga cctcggtctc ccaggccgtc atccagcgtt
     1321 cgagcgcgaa cggttcgaac tgcaaagtgt gctccatctc caatgctgcg ggaactgcgc
     1381 gagtcgttag cttcggtaag ggcagacctc cgtgtctgcc ctcgttggcc tattgccggg
     1441 ccgcttcgta cgccgctgat gtccccaatg ccccgtcgcc cttgtcctag tggcgttggc
     1501 ataggggaga cagggcagac acaggggtct gcccctacag gataggcgcg ggttgcgcgg
     1561 ttggctaccg cgccacgatg ttgatcaggc gccgcgggac gtagacgacg cgctgcacct
     1621 gtttccccgc ggtgaactcg gccacgcgcg ggctggccat ggcctgctcg atcgcgttct
     1681 cctggctgat gtcggcaggc acgtccaggt ggtcgcgtac cttgccgttg acctgcaccg
     1741 gcagctcgat cagctcttcc ttgaccagct ccgcgtcgaa ggtcggccac aactgctgat
     1801 gcacgctata ggtctgcccc agccgctgcc acagttcttc ggccagatag ggcgcaatcg
     1861 gcgccagcat cagcgtcagc gcctccagcg cctcgcgcca ggcgcgcgtc gacgccacgt
     1921 ccgtgtcctt gcgcggcgcc agctcgttct gcagctccat cagccgcgcg atcatcgtgt
     1981 tgaagcggaa gttctcgatg tcttcggtcg ccgcttggat cgtgcggtgg gtgatccggc
     2041 gcaactcatg ggcctccgtc gtcgccgcct ccggcgccgg gttgtcggcg gtctcctgta
     2101 ccagcgacca cacgcggcgc acgaagcgtt cgactccggc gatgccgcgc gagttccagg
     2161 ggccgccctg gtcccatggg ccgatgaaca tcaggtacat gcgcaccacg tcggcgccca
     2221 attgcgagac caggtcgtcg gggtcgacga cgttgccccg gctcttggac atcttctcgc
     2281 tgttctcacc caggatgatc ccctggttcg ccaggcgcac gaagggctcg ccgtagtcca
     2341 ccagccccat gtcccgcatc gccttggtaa agaagcgcgc gtacatcaga tgcagaatgg
     2401 cgtgttcgat accgccggtg tagcgatcga caggcatcca gtagtcggcc ttctccttcg
     2461 taaacggcgc ggtgtcgtcg tgcgggctca ggtagcgata ctggtaccag gacgagtcga
     2521 cgaaggtgtc catggtgtcg gtctcgcgcc gcgccggccc gccgcacctg gggcaggtgg
     2581 tgctgacgaa ctcttcgtcg gtcaccagcg gcgactgccc ggtcggggtg aactcggcgt
     2641 cctgcggcag gattaccggc agttgatcct ccggcaccgg caccacaccg caggtgtcgc
     2701 agtagacgat cgggatcggc gtgccccagt agcgctggcg cgagatcagc cagtcgcgca
     2761 ggcgataggt gacctcgccc tggccgatcc ccgcctcctt cagccaggcg atcacgcggt
     2821 cgatcgcctg gccgcccgcg gtgggagtgc cgttgaacgg ccccgagtgg accatcacgc
     2881 cctcgccggc gtaggcctcg tttatctcca ccgcatccag cgtctcgcct tcgggctcga
     2941 tgacgacctc gatcggcagg ccgaacttgc gcgcgaactc gaagtcgcgc tgatcgtgcg
     3001 ccggcaccgc catgatcgct ccggtgccgt agcccatcag cacgtagtcg gcgatccaga
     3061 tcggcacggc ctcgccgttg actgggttgg tggcgtaggc gccgatgaac tcgccggtct
     3121 tctccttgtc ggtcgaggtg cgctcgatct cggtctcccg ccgcgcggca tcgacgtagg
     3181 ccgccacggc ggcgcggcgc tcgggcgtcg tcaacttctc gaccagcggg tgctccggcg
     3241 ccagcaccat gaaggtggcg ccccacagcg tatcgggccg ggtggtgaag atctcgatcg
     3301 gctcgccgat ggcggtgggg aagcgcaccc gcgcgccctc cgagcgcccg atccagttgc
     3361 gctgcatcgt ccgcacgcgc tcgggccact cgatccctgc gaagctcagc agctcatcgg
     3421 catacttggt gatgcggaag aaccactgct ccaggtcgcg acggtagacc tcgctctcgc
     3481 aacgctcgca gcggccgttg atcacctgct cgttggccag cgaggtctga caggtggggc
     3541 accaccagac gggcgccatg gcgcgatagg ccagcccgcg tttgtagaac tccaggaaga
     3601 accactggtt ccacttgtag tactccggca cacaggtgat gacctcgcgc gaccagtcga
     3661 tcatcgtgcc catcgagcgc atctgctgct ccatgcgggg gatgctggtc atggtccagg
     3721 tgtaggggtg gataccgcgc ttgatggccg cgttctcggc cggcagcccg aaggcgtcga
     3781 agcccatcgg gaagagcacg ttgtagccct gcatgcgctt gtagcgcgcg ccggcgtcgg
     3841 acggcgtcat ggcgtaccag tggccgatgt gcaggtcgcc cgagggatag gggtacatcg
     3901 tgacgtggta ccacttgggg cgcgagtcat cctcgaccgt ctggtacagc ccggtctctt
     3961 cccagatgcg ctgccacttg tcttcgatcg cgttggggtc gtagcgctcc ggccggacga
     4021 cccgctcggt ggttgccatg ttgtgcctcc tcgatccgcg gcggctgtgc agctacgaaa
     4081 ccccaggccg cgccgcgatt cagccggtta aacaaaagag cctccgtcct gatcagggac
     4141 gaaagctcgg cttccgcggt accaccctgc ttgataggcg aggtctggtg gagctgaggg
     4201 gattcgaacc cctgacccca acactgccag tgttgtgctc tcccagctga gctacagccc
     4261 cgcgccgtgc ctggcctatc cgcttatgga cccgctaacg ggggtaaacc ggcgccggct
     4321 aactcttcac cggcgcggct cccgggcgag ttcgaccgct ctggctgcct cgcaccatcc
     4381 ggcagctcgc tgtgcggcgc ggcctactac tcccgttcgt cgccattcac aggctaaatt
     4441 acgcggggag acgcgctccc cgcgagcggt aattctagca ccggccgagc gccgctgtca
     4501 aacgcgcgcc ggcgcgaagg cccgggggcc ttgcccagga ggggcgacag cccccaagcg
     4561 ccttcgtcta cggcccggcg acggtctcgc cgggcgcgta gaggttgagc cacacattga
     4621 aggccgcgat ggcgccgttg tcgccggtgg cgaaggtggc cgtgcgccca tcgagcgcca
     4681 ccaccgagcg ctcgccgacg accgtggccg taccgccgtc aagcgagagc gcggtttgct
     4741 cgctgatgcc gaagacgagc gtctgcgggt cggcctgcgc cagagcgtag agccgtcccc
     4801 agcgctggtc tgtggtcaac cgcggctcga aggcagcgtg gacgtagccc aggcccggct
     4861 cgacctcggc atagtcgctg ctgaaggccg cgatcgactg actctgcagg ttctgcgtgg
     4921 tcgggtcggc gttggtcacg tactgctcac ccatcgccgg cgtcatggcg cggtcggtca
     4981 gcacgaccgg cgcggtgtcg atcgcctgct cgaccagggc acggaaggtg gcatcggcca
     5041 gcggacgcgc catctgcgat tggtcgccgc ccaccaggat cacaccagcc gcgccgtcga
     5101 ccacatccgg tgcgacgcgc gaccagccgg tcgtgtcctc gaaattgagt atggtgattt
     5161 gatccttcga ctgtgaccag cccagcttct tcaacgcctg cgcgtaggtg ttggcggtgc
     5221 tgctcgcgtc cccggtcgag aagaacccgg ccacaaccag cacgacgcgg tgagatcccg
     5281 tcgcccgcgc ctggctgacg aagctcgaca gcccactgcc cccggcgagc aggtcgccgc
     5341 ccagcagtaa ggtgccgggg ccgggcgccc gcagcacgcc ggcgtcccac gcagttggcg
     5401 caatggcagg ctgggcgaac ccagcgaccg agagccggcg gccgacgacg ttatagctgt
     5461 acgggccggg aggcatgatc tgcgtcagaa catcgcgcgc cgagagcgtc tgttgtgacc
     5521 cgacccaatc gtgcgtcgcc tgggagaaat cgatgatcgc gatcgagctg gcgccggtga
     5581 catcgtcgag cacggtgtcg tcggtcaggt ggacgctggt ggcatagtcg acgccgacgc
     5641 cgacccggcc attgccgccg ta
//
LOCUS       DASUBI010000335         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_898324, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000335 DASUBI010000000
VERSION     DASUBI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898324"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFU72_06480"
     CDS             complement(<1..324)
                     /locus_tag="VFU72_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5423166.1"
                     /translation="MANGDSGRGGAGVQSRIEIPSGLTEDDLRGMYELMALARALDER
                     MWVLNRAGEAPFVISVQGHEAAQAGMAFALKPGRDVLAPYYRDLALVLHFGITPRSVM
                     CSLFGK"
     gene            complement(369..1766)
                     /gene="lpdA"
                     /locus_tag="VFU72_06485"
     CDS             complement(369..1766)
                     /gene="lpdA"
                     /locus_tag="VFU72_06485"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5423167.1"
                     /translation="MPDEMFDVVLLGGGTGGYVAAIRGGQLGLKVAVVERDKVGGTCV
                     HRGCIPAKALLKSGSVVHLVQNASDFGITASGWEINYAQASSRSEKIVNQMYNGIKFL
                     FRKHNVTTIEGTGRLKDNHTILVDKSDGGTQEVSAKAIIVDTGSRPRPIPGIEFDGQR
                     VVNSDHTTKMDWFPKRVIIRGGGATGVEFATVYRDFGAEVTLVGRIVPNEDQEVSRQL
                     TRSFQKAGIKIIPNYRPTADDFDITEGGVALRVRGDGGKEERVEADTLLVATGRQGNI
                     EDIGLEQLGIQTEKSYIMTDEYFRTGAEGVYAIGDVIGKSMLAHTAMHQGIIVMELLA
                     GEKPMPLDYNRVPNVTFCHPEIGSLGLTEEQAKEAGYAVKVGKFPLRANGKAVIEDVA
                     DGFIKMVADQETNDILGVHMIGGNATEIIGEAALAKLLEATPWEVGLSVHPHPTISEV
                     MGEAALAVDNLQIHA"
     gene            2052..>2363
                     /locus_tag="VFU72_06490"
     CDS             2052..>2363
                     /locus_tag="VFU72_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU5423168.1"
                     /translation="MVTTIEMTVPAATWTRMAAKANRERLRAYRLNDDPRSWAVTSHS
                     SDDIAYEVTILDGDLLCSCRGSAFHPYCKHRALVLKELGVLEEAGGEEELVPGRVLAA
                     LQ"
BASE COUNT          362 a          798 c          764 g          439 t
ORIGIN      
        1 cttgccgaac aggctgcaca tgaccgagcg cggggtgatg ccgaagtgga gcaccagcgc
       61 caggtcgcga tagtagggcg cgagcacatc gcgccccggt ttgagggcga aggccatgcc
      121 ggcctgcgcc gcctcgtgcc cctgcacgga gatcacgaag ggcgcctcgc cggcccggtt
      181 gagcacccac atgcgctcgt cgagcgcgcg ggccagggcc atcaactcgt acatgccgcg
      241 aaggtcatcc tccgtgaggc cggacggaat ctcgatgcgg ctctgtactc cggccccgcc
      301 gcgaccggaa tcgccgttcg ccatgtcagc ctcctctggc tctcggagcc ctcgttggct
      361 ccgcgttctc aggcgtgaat ctgtaggttg tcgacggcca gcgcggcctc gcccatcacc
      421 tcggagatcg tcggatgcgg gtgtacgctc agtccgacct cccagggcgt cgcctcgagc
      481 agcttggcca gcgccgcctc gccgatgatc tccgtggcgt taccgccgat catgtggacg
      541 ccgaggatgt cgttggtctc ctggtcggcc accatcttga taaagccgtc cgccacgtct
      601 tcgatcacgg ctttgccgtt ggcgcgcagc gggaacttgc cgaccttgac cgcgtagccg
      661 gcttctttcg cctgctcctc ggtcagcccc aggctaccga tctcggggtg acagaaggtc
      721 acgttcggga cccggttgta gtcgagcggc atcggctttt cgccggctag cagctccatc
      781 acgatgatcc cctgatgcat ggcggtgtgg gccagcatcg acttgccgat cacgtcgccg
      841 atagcgtaga ccccctcggc acccgtgcgg aagtactcat cggtcatgat gtaggacttc
      901 tcggtctgga tgccgagctg ctccagcccg atgtcctcga tattcccctg gcgccccgtc
      961 gcgaccagga gcgtatcggc ctcgacgcgc tcctccttgc cgccgtcgcc gcgcacccgc
     1021 agcgcgacgc cgccctcggt gatgtcgaag tcgtcggcgg tcgggcggta gttcgggatg
     1081 atcttgatgc cggccttctg gaaggagcgg gtcaattgcc gggagacttc ttgatcctcg
     1141 ttcggcacga tgcgcccgac cagggtcacc tccgcgccga agtcgcggta gaccgtggcg
     1201 aactcgacgc cggtcgcgcc ccctccgcga atgattactc gcttggggaa ccagtccatc
     1261 ttggtggtgt ggtcgctgtt gacgacgcgc tgcccgtcga actcaatccc cgggatcggc
     1321 cgtgggcgcg agccggtgtc gacgatgatc gccttcgcgc tgacctcctg tgtgccgccg
     1381 tcgctcttgt cgaccaggat ggtgtggttg tccttgaggc ggccggtgcc ctcgatagtg
     1441 gtgacgttgt gcttgcgaaa gaggaacttg atgccgttgt acatctggtt gacgatcttc
     1501 tcggagcggc tggaggcctg cgcgtagttg atctcccagc cggaggcggt gatgccgaag
     1561 tcggaggcgt tctgcaccag atgcacgacg gagccgctct tgagcagcgc cttggcgggg
     1621 atgcagccac ggtgcacgca cgtcccacca actttgtccc gctcgacgac cgccactttc
     1681 agaccgagct gcccgccgcg aatcgcggcg acatagccgc cggtaccacc gccgagcaga
     1741 acgacgtcga acatctcgtc aggcattccg attcccctct tatctggcgc ttggcctcgc
     1801 cggggctcac cccacgcggc ggccacccca cggcaatcgc ccggggtgtg gccggtgcag
     1861 atggggctgg tatgccacca tgcaaggttg caccttccgt gccacggcgc gcccatcccg
     1921 cgagcggcac gccttatcct cacccgtatt aaacaccctg tgcgctcgcg atgaggtggt
     1981 tagaacagat gtccgtatgg cataattggc gctcgacggc agggtttcgg tcttcgacag
     2041 gaagcgttga catggtgacg acgatcgaga tgacagtccc ggccgcgacc tggacccgga
     2101 tggcggccaa ggcgaaccgc gagcggctgc gcgcctaccg gctcaacgac gacccacgct
     2161 cctgggcggt caccagccac agcagcgacg acatcgccta cgaggtgacg atcctcgacg
     2221 gtgatctgct ctgctcctgc cgcggcagcg cctttcaccc ctactgcaag caccgcgcgc
     2281 tggtgctcaa ggagctgggt gtgctcgaag aggcgggcgg cgaagaggag ctcgtgccgg
     2341 gccgggtgct ggccgcgctc cag
//
LOCUS       DASUBI010000336         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_424035, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000336 DASUBI010000000
VERSION     DASUBI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424035"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1597)
                     /gene="narH"
                     /locus_tag="VFU72_06495"
     CDS             complement(26..1597)
                     /gene="narH"
                     /locus_tag="VFU72_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEU5423169.1"
                     /translation="MNIRAQVSMVFHLDKCIGCHTCSVACKNIWTDRKGTEYMWWNNV
                     ETKPGTGYPTLWEDQSKYKGGWERTNGNGNGKANGTSNGDGAAQGADGLRLRLHGRLG
                     GLKNIFSNPYLPTMDDYYEPWTYDYEHLFNAPEGDDQPTARAVSLVSGKPMDTIEAGP
                     NWDDDLGGSPVYAANDPNLEKLSPEEREQMAEVERLVFFYLPRICNHCLNPGCVAACP
                     AGAIYKRGEDGIVLVSQEKCRGWRMCISGCPYKKVYYNWATGKSEKCILCFPRLESGQ
                     APACFHSCVGRIRYLGILLYDADGIRATAGLPDAELVEAQRALIQDPFDPAVIAAARE
                     NGVADQLIDAAQKSPVYKFVKRWQLALPLHPEFRTRPMLFYVPPMLPVLASVQNGHYD
                     VAGADSEGLGALLTSLERARMPLRYMASLFAAGNEEIVEAVYRKLIAVRVYKRAQKVK
                     DYPPEEARRALEAGRTTAEEAEAIFRLTSLPTFDERFVVPPLAREQAIEQTLDPFTHK
                     PATGFGFRQPPERRF"
     gene            complement(1650..>3230)
                     /locus_tag="VFU72_06500"
     CDS             complement(1650..>3230)
                     /locus_tag="VFU72_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU5423170.1"
                     /translation="GNAIQSSAKGHEFFLRHYLGTHDNIDADERARDSVRTVTVREPA
                     PRGKMDLVVDLNFRMDSSALYSDIVLPAAFWYEKNDLNTTDLHSFIHPLGQAVPPVWE
                     SKTDWEIFKALSRKVSELAPGVFPEPVTDLVTLPLRHDTPDELAQTEPRDWRAGECAP
                     IPGQTMPHFHLVERDYTRLYQRFISLGPRIREDGVNGNGVHIPIARFYDELLQNPVGG
                     SPDPRHMRCVEWDGQRYPSLEDALDAANTLLHLAPESNGEVAYAAFQEEERHTGVPLA
                     DLAEKSRSVRMNFGDITRQVRRNLTSPCWSGIVNDGRAYAAWCLNIERLVPWRTLTGR
                     QHFYIDHPWYLDFGEGLPVYKPKLNPVRTGDIVRSPVDESCLVLNYITPHGKWNIHST
                     YKDNHRMLTLSRGMDPIWINDKDADKIGLNDNDWVEVYNDNGVVVTRAAVSARVHPGT
                     ALYYHAVERTVYIPKSQVRGKRRAGGHNSLTRTRINPVQLAGGYGQLSYSFNYWGPIG
                     IMTRDTYCVVRKLEQLEW"
BASE COUNT          457 a         1032 c         1156 g          585 t
ORIGIN      
        1 agccgcgcgc gttcgcagac catggttaga acctccgctc gggtggctgc cggaagccga
       61 agccggtcgc cggcttgtgc gtgaacgggt cgagcgtctg ctcgatcgcc tgctcgcgcg
      121 ccagcggcgg cacgacgaag cgctcgtcga aggtcggcaa ggacgtgagg cggaagatcg
      181 cctccgcttc ctccgccgtg gtccggccgg cctccagcgc ccgccgcgcc tcctccggcg
      241 gatagtcctt gaccttctgc gcgcgtttgt agacccgcac cgcgatcaac ttgcggtaga
      301 ccgcctcgac gatctcttcg ttgccggccg cgaacaggct cgccatatag cgcagcggca
      361 tgcgggcgcg ctcgagtgac gtcaagagcg cccccagccc ctcactgtcg gcgccggcga
      421 cgtcgtagtg gccgttctgg acgctggcca gcaccggcag catcggcggc acgtagaaga
      481 gcatcggccg ggtgcggaac tcgggatgga gcggcagtgc cagttgccag cgcttgacga
      541 atttgtagac cggcgatttc tgcgcggcgt cgatcaactg gtcggcgacg ccattctcgc
      601 gcgcggcggc gatgacggcc gggtcgaacg ggtcctgaat gagcgcccgc tgcgcctcga
      661 ccagctcggc atcgggcagc ccggccgtgg cgcggatgcc atcggcgtcg tagaggagga
      721 tgccgaggta gcggatgcgc ccgacgcacg agtggaagca ggccggcgcc tggccactct
      781 ccaggcgcgg gaaacagagg atgcacttct cggacttgcc ggtggcccaa ttgtaataga
      841 ccttcttgta ggggcagccg gagatgcaca tgcgccagcc gcggcacttc tcctggctga
      901 ccagcacgat gccgtcctcg ccgcgcttgt agatcgcgcc ggcggggcag gccgccacgc
      961 agccagggtt gaggcagtgg ttgcagatcc gcggcaggta gaagaagacg agccgctcca
     1021 cctcggccat ctgctcgcgt tcctcggggc tcagcttctc caggttgggg tcgttggccg
     1081 cgtagaccgg cgagccgccc aggtcgtcgt cccagttggg gccggcctcg atggtatcca
     1141 tcggcttgcc gctgaccagc gacaccgcgc gggcggtggg ctggtcgtcg ccctcggggg
     1201 cgttgaagag gtgctcgtaa tcgtaggtcc acggttcgta gtagtcgtcc atggtcggca
     1261 ggtaggggtt cgagaagatg ttcttgagcc cgcccagccg cccgtgcagg cgcagccgca
     1321 ggccgtccgc cccctgcgcc gctccatcgc cgttgctcgt gccgttggcc ttgccgttac
     1381 cgttgccgtt ggtgcgctcc cagccgccct tgtacttgct ctggtcctcc cagagggtcg
     1441 ggtagccggt gcccggcttg gtctcgacgt tgttccacca catgtactcg gtgcccttgc
     1501 ggtcggtcca gatgttcttg caggcgacgc tgcaggtgtg gcagccgatg cacttgtcga
     1561 gatgaaagac catcgagacc tgcgcgcgga tgttcatcga tcggtccctt tccgtccgcg
     1621 agacgggcgc ggtcgcggcg ctcgcccggt taccactcca gctgctccag cttgcgcacc
     1681 acgcagtagg tgtcgcgggt catgatgccg atcggtcccc agtagttgaa gctgtagctg
     1741 agctggccgt agccgccggc cagttggacc gggttgatcc gcgtgcgtgt caggctgttg
     1801 tgcccgccgg cgcggcgctt gccccgcacc tgcgacttgg ggatgtagac cgtgcgctcg
     1861 acggcgtggt agtagagcgc ggtgccgggg tgcacgcgcg cgctgaccgc cgcgcgggtc
     1921 acgaccaccc cgttgtcgtt gtagacctcg acccagtcgt tgtcgttgag gccgatcttg
     1981 tcggcgtcct tgtcgttgat ccagatcggg tccatgccgc gcgagagcgt cagcatgcga
     2041 tggttgtcct tgtaggtcga gtggatattc cactttccgt gcggggtgat gtagttcagc
     2101 accaggcagc tctcatcgac cgggctgcgc acgatgtcgc cggtccgcac cgggttgagc
     2161 ttcggcttgt agaccggcag tccctcgccg aagtccaggt accaggggtg gtcgatgtag
     2221 aagtgctggc gcccggtcag tgtccgccag gggaccagcc gctcgatgtt gaggcaccag
     2281 gcggcgtagg cgcggccgtc gttgacgatg cccgaccagc aggggctagt gaggttgcgc
     2341 cgcacctggc gggtgatatc gccgaagttc atgcgcacgc tgcgcgactt ctcggccagg
     2401 tcggccagcg gcaccccggt gtgccgctcc tcctcctgga aggcggcgta ggcgacctcg
     2461 ccgttgctct cgggggcgag gtggagcagc gtgttggccg cgtcgagcgc gtcctccaga
     2521 ctcgggtagc gctggccgtc ccactccaca cagcgcatgt gccgcgggtc gggcgaccca
     2581 ccaacggggt tctgaagcag ctcgtcgtag aagcgggcga tcgggatatg cacgccgttg
     2641 ccgttgacgc cgtcctcgcg gatgcgcggg ccgagtgaga tgaagcgctg gtagaggcgg
     2701 gtgtagtcgc gctcgaccag gtggaagtgc ggcatggtct ggccggggat cggcgcgcac
     2761 tcgcccgccc gccagtcgcg cggctcggtc tgcgccagct cgtccggcgt gtcgtgccgc
     2821 agggggagcg tgacgaggtc ggtcaccggc tccgggaaga ctccgggcgc cagctccgag
     2881 accttgcgcg agagcgcctt aaagatctcc caatcggtct tcgactccca gacgggcggc
     2941 actgcctgcc cgagcgggtg gatgaacgag tgcaggtcgg tggtgttcag gtcgttcttc
     3001 tcgtaccaga aggcggccgg cagcacgatg tcggagtaga gcgccgaaga atccatgcgg
     3061 aagttgaggt cgacgacgag gtccatcttg ccgcgcggcg ccggctcgcg gacggtgacg
     3121 gtgcgcacgg agtcacgcgc gcgctcgtcg gcgtcgatgt tgtcgtgggt gccgaggtag
     3181 tggcgcagga agaactcgtg acccttggcg ctggattgaa tggcgttgcc
//
LOCUS       DASUBI010000337         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_340854, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000337 DASUBI010000000
VERSION     DASUBI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340854"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFU72_06505"
     CDS             <1..448
                     /locus_tag="VFU72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5423171.1"
                     /translation="DYCSDMQRTYYFLRDGETEAPAPVLHAFEVVSGAIQEAAALLKP
                     GVQGWEVDQVAREIFARAGAEEWGYALGHQLGRACHDGGCLLGPRWERYGERPYDPVE
                     ENEVYTLEIGVSVPGYGRVSLEEDVVVTQDGCEFLSEPQRRPILIG"
     gene            475..1641
                     /locus_tag="VFU72_06510"
     CDS             475..1641
                     /locus_tag="VFU72_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5423172.1"
                     /translation="MPDTQTLLAYADEAVDRYLLELAEIVDIDSGTRDKAGVDRAGSF
                     LARLLTGIGCELRGFPNPELGDNFTATLRGGGERRVVLLGHVDTVYPRGTVAERRFTI
                     SERRAYGPGVLDMKGGLILGYHAMRILGEVARDEFAELVFVANGDEEIGSPSSRGQIE
                     AEARRADAVLVLEPGHEPGSVLAERKGVGWYAVEVFGRASHAGAAPEEGASAVLELAH
                     KIIALHGLNDPGSGTTVSANVIRGGTARNVIPADAHAAVDVRVTTPEEAARVDAAIQA
                     LARRATVAGTRTVISGGLNRPPMAPRQSTQPLLECARRLDRQLGLEFSALGSGGGSDG
                     NFTAALGVPTLDGLGPVGRNAHSVDEYIELDSIPARLALLTGLILGVPRRPGRA"
     gene            1920..>2340
                     /locus_tag="VFU72_06515"
     CDS             1920..>2340
                     /locus_tag="VFU72_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5423173.1"
                     /translation="MAGWFEVRKLPDGVVGLGEPGHDEDVKSYLVRGSELALLVDSGT
                     GIGDIRSVVDQLVTTPVLLVNSHAHWDHIGGNWQFPRVWIHEAEADRLGEQLPRERLR
                     RFVAPERFVGAPPEGFDAQRFTIRPSQAERRLREGDEI"
BASE COUNT          346 a          794 c          851 g          349 t
ORIGIN      
        1 cgactactgc tccgacatgc agcgcaccta ctacttcctg cgcgatggcg agaccgaagc
       61 gccggcgccg gtgctgcacg ccttcgaggt cgtctccggc gcgatccagg aggcagccgc
      121 gctgctgaag cccggcgtgc agggctggga ggtcgatcag gtggcgcgcg agatcttcgc
      181 gcgcgccggc gcggaggagt ggggctacgc gctcggccat cagctcggac gcgcctgcca
      241 cgacggcggc tgcctgctcg gcccgcgctg ggagcgctac ggcgagcggc cctacgaccc
      301 ggtcgaggag aacgaggtct acacgctgga gatcggcgtg agcgtccccg gctacggtcg
      361 ggtcagtctg gaagaggacg tggtcgtcac ccaggacggc tgcgagttcc tctccgagcc
      421 gcagcgccgg ccgatcctga tcggctagca atagagcgga aggacggttc cgacgtgccc
      481 gatacccaga cgctcctggc gtatgccgat gaggctgtcg accgctacct gttggagctg
      541 gccgagatcg tcgatatcga cagcggcacg cgcgacaagg cgggggtcga tcgggcgggg
      601 agcttcctgg cgcgtctgct gacgggcatc ggctgtgagc tgcgtggctt ccccaaccca
      661 gagttgggcg acaacttcac cgccaccctg cgtggcggcg gcgagcgccg agtcgtcctc
      721 ctcgggcacg tcgacacggt ctacccgcgt ggcacggtgg ccgagcggcg cttcaccatc
      781 agcgagcgtc gcgcctacgg tccaggggtg ctcgacatga agggcgggct gatcctcggc
      841 taccacgcca tgcgcatcct gggagaggtc gcgcgcgatg agttcgccga gctggtcttc
      901 gtggccaacg gcgatgagga gatcggctca cccagctcgc gtgggcagat cgaagctgaa
      961 gcgcggcgtg ccgacgcggt actggtgctg gagccggggc acgagccggg cagcgtgctg
     1021 gccgagcgca agggtgtcgg ctggtatgcg gtcgaggtct tcggccgcgc ctcgcacgcc
     1081 ggtgccgcgc ccgaggaggg ggccagcgcg gtcctggagc tggcgcacaa gattatcgcc
     1141 ctgcacggac tcaacgaccc cggttcaggc acgacggtct cggccaacgt gattcgcgga
     1201 ggcacggccc gcaatgtgat cccggccgac gcgcatgccg ctgtcgacgt gcgggtgacc
     1261 acgccggagg aggccgcgcg cgtcgacgcg gcgatccagg cgctcgcccg ccgcgccact
     1321 gtggcgggca cacggactgt gatctcgggt ggtctcaacc gcccgccgat ggcgccgcgg
     1381 cagagcacgc agccgctcct ggaatgcgct cgccggctgg accggcagct tgggctggag
     1441 ttctccgcgc ttggcagtgg cggcggcagc gacggcaact tcaccgccgc gctgggcgtg
     1501 cccacgctcg acggtctcgg cccggtcggc cgcaacgcgc acagcgtcga cgagtacatc
     1561 gagctggact cgatccccgc gcggctggcc ctcctgaccg gcctcatcct cggcgtgccg
     1621 cgccggcctg gccgagccta ggcggtggcc gtcgaccacg ttggttgccg ggtgaggacg
     1681 gatccttcga ctgcgctcag gatgacaggg agagtgagag gccgacgacg aggactgtct
     1741 tcgtccggca ggcccctccc taacgctgtc atcctgagcg tagtcgaagg atccgtcctt
     1801 gatgattccc atgctccgcg atggccaccg cgcccggcag ccgtccgatt cctcagcccg
     1861 cacccagacg ctcccgtata atcgggccgc gagtgccgcc gacggatagg gagacgctga
     1921 tggccggttg gttcgaggtg cgcaaattgc cggatggggt ggtcggcctg ggcgagccgg
     1981 gccacgacga ggacgtcaag tcgtatctgg tgcgcggcag cgaactggcg ctcctcgtcg
     2041 acagcggcac cggcatcggc gacatccgtt ccgtggtgga tcaactggtc accactcccg
     2101 tgctcctcgt caatagccac gcccactggg accatatcgg cggcaactgg cagttcccgc
     2161 gagtctggat ccacgaggcg gaggccgacc ggctcggtga gcaactgccg cgcgagcgcc
     2221 tacgccgctt cgtggcgccg gagcggttcg tcggcgcgcc gccggagggc ttcgacgcgc
     2281 agcgcttcac catccggccc agccaggccg agcgccggct gcgcgagggg gacgagatcg
//
LOCUS       DASUBI010000338         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_772078, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000338 DASUBI010000000
VERSION     DASUBI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772078"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFU72_06520"
     CDS             complement(<1..1217)
                     /locus_tag="VFU72_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5423174.1"
                     /translation="MADKFEKFTERARKVLTLAQEEAHRFNHNYIGTEHLLLGLVREG
                     DGVAARVLANMGVQLPKVRSAVEFIIGRGDSMIVGEIGLTPRAKKVIELAVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRQQVLQVVSQSSGYQQKAQQTKT
                     PYMDALGFDLTEAARSSKLDPVIGRQSEIERVMQILSRRTKNNPALIGEPGVGKTAIV
                     EGLAQRIISGDVPEPLQNKRLVALDIGALVAGTKYRGEFEERLKKIVGEVKETGAILF
                     IDELHTLVGAGAAEGAVDAANILKPALSRGEVQTIGATTMDEYRKYIERDAALERRFQ
                     PVQVDEPSIEDTIAILTGVRPLYEEHHRLKIADDALVAAAQLSSRYVPDRRLPDKAID
                     LIDEASSRVRLQKA"
     gene            complement(1396..2727)
                     /gene="rpsA"
                     /locus_tag="VFU72_06525"
     CDS             complement(1396..2727)
                     /gene="rpsA"
                     /locus_tag="VFU72_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU5423175.1"
                     /translation="MDRTQQTVSTDDGGVELPVSLMEEFLNDPLHDYRVLSYGDVLEG
                     QLMHVDRDELLVDIGSKSEGVIPAREFSSLNEEERRRLTVGDQVLVFVVQPENQEGQA
                     VLSIDRALAEKSWRRLQDVYDAGEVIEAEVTNYNKGGLLVNLDGVRGFVPASQVTEIR
                     GGDEGSKQADMARLIGSKLSLKIIEINRHRNRLILSERQALQERRDVMKERLIEELHA
                     GETRRGRVTSITDFGAFVDIGGADGLVHLSELSWSRVKHPSEVLHVGEEVDVYVLGIN
                     AEEKKIALSIKRTQPEPWSRVAAKYEVGQLVIGTVTQLANFGAFARIEDGIEGLIHVS
                     ELSEQRIEHPKQVVHEGQDLILRIIRIDPARRRMGLSLRRALEATDEEVEAALGDDAV
                     ALKQELLAQEIEPDESEVEYEPEDAPASDAAEHATAAEAPRADNGEEDSEG"
     gene            complement(3136..3669)
                     /locus_tag="VFU72_06530"
     CDS             complement(3136..3669)
                     /locus_tag="VFU72_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5423176.1"
                     /translation="MLQPAESERFATPLKIGVVADTHLGAEPRPLPEALVRELRRVDL
                     ILHAGDVCSAAALALFAEIGPPLRAVVGNNDSMALRDLLPRARRFQFGLFSAGMIHGD
                     GFGRLTARQAAESIFAGQVDLGIFGHSHRPLCQRVDGLLLFNPGSALQRRWQPRHTLG
                     MLRISQEIDAEIVELPY"
     gene            complement(3669..4016)
                     /locus_tag="VFU72_06535"
     CDS             complement(3669..4016)
                     /locus_tag="VFU72_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5423177.1"
                     /translation="MARIVGLLVANGLTLWLLARFMPRQVSYDQTWQTIIAFAAVLTL
                     LDLILAPILRLITWPLSCLTLGLFSLIVSGAVFYLGGRLAGGVEITYLGALVGGIVAG
                     IVSSTLTTALRKR"
     gene            complement(4009..>4485)
                     /locus_tag="VFU72_06540"
     CDS             complement(4009..>4485)
                     /locus_tag="VFU72_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HEU5423178.1"
                     /translation="HRFWLGLTLQDPATKLRLQEYQNALSIIRTHPLFGVGFGAAPSI
                     DQQTGVSSIYLSIAERAGFLGVAVFLFALGAVVLAGWRAWREAPEGAYNDLLLGLLAA
                     LASALVVGVFDHYYFNIMFPHMAALFWMTCGLILALATMPIAERWDAVMEPGGVDG"
BASE COUNT          723 a         1569 c         1428 g          765 t
ORIGIN      
        1 gccttctgaa gccggacgcg cgatgaggct tcgtcgatca ggtcgatcgc cttgtcgggc
       61 aggcgccggt cggggacgta gcgcgacgag agctgcgccg ccgcgaccag ggcgtcgtcc
      121 gcgatcttga gccggtggtg ctcctcgtag agcgggcgaa cgccggtcag aatcgcgatc
      181 gtatcctcga tcgacggctc atcgacctga acgggctgga agcgccgctc cagggccgcg
      241 tcgcgttcga tgtacttgcg gtactcgtcc atcgtggtgg cgccgatggt ctgcacctca
      301 ccacgcgaga gcgccggctt gaggatgttg gccgcgtcga ccgcgccctc cgccgcgccg
      361 gctcccacca gtgtgtgcag ctcgtcgatg aagaggatgg cgccggtctc cttgacctcg
      421 cccacgatct tcttgagccg ctcctcgaac tcaccgcggt acttggtgcc ggcgaccagc
      481 gcgccgatgt cgagcgccac cagccgcttg ttctgcagcg gctcgggcac atcgcccgag
      541 atgattcgct gcgccagacc ttcgacgatg gccgtcttgc cgacgcccgg ctcgccgatc
      601 agcgccgggt tgttcttggt gcggcgtgag agaatctgca tcacgcgctc gatctccgac
      661 tgccggccga tgaccgggtc gagcttgctc gaccgggcgg cctcggtcag atcgaagccc
      721 agtgcgtcca tgtatggtgt cttcgtttgc tgtgcctttt gctggtagcc gctgctctgg
      781 ctgaccactt gcaggacctg ctggcgcacc ttctccaggt tgacacccag gctctccagc
      841 acgcccgccg cgataccctc gccctcacga acgaggccga gcaacaggtg ctcggtgccg
      901 atgtagtggt ggttgagtcg ccgcgcctcg tccaccgcca gctcgatgac cttcttggcg
      961 cgcggcgtca gcccgatctc accgacgatc atcgagtcgc cccggccgat gatgaactcg
     1021 accgcgctgc gcaccttcgg cagttgcacg cccatgttgg ccagcacgcg ggcggcgacg
     1081 ccgtcgccct ccctgacgag gccgaggagc aggtgctcag tcccgatgta gttgtggttg
     1141 aagcggtgcg cctcttcctg cgccagcgtc agcaccttgc gcgcccgctc ggtgaacttc
     1201 tcgaatttgt ctgccattgc tctctcctcg aggcctcagt agtggcgcgg ctgcctaccg
     1261 tccccacctt agcaccgatc ccagaaatgg ctagcccccg cacaccgggt ggtagggggc
     1321 ttcgccatct accggcgcgc gtcaacgacg tgccggttgg tttctcatgt gcgagggtct
     1381 ggcggggact cccggctagc cttcggagtc ttcctcacca ttgtcggccc gtggagcttc
     1441 agctgcagtg gcgtgctctg ctgcgtcgga tgccggtgca tcctcgggct cgtattcaac
     1501 ttcggattcg tccggttcga tctcttgcgc cagcagttcc tgcttcagcg cgaccgcatc
     1561 gtcacccagc gccgcctcga cttcctcgtc ggtcgcctcc agggcccggc ggaggctgag
     1621 ccccatgcgc cggcgcgcgg gatcaatacg gatgatacgc aagatcaggt cctgcccctc
     1681 gtggacgacc tgcttcgggt gctcgatccg ctgctccgaa agctcggaga cgtggatcag
     1741 accctcgatg ccgtcctcga tgcgcgcgaa ggcaccaaag ttcgccaact gcgtgacggt
     1801 gccgatgact agctggccga cctcgtactt ggccgccacc cggctccacg gctccggctg
     1861 cgtgcgtttg atgctcaggg caatcttctt ctcctcggcg ttgatgccga ggacgtagac
     1921 gtcgacttcc tcgccgacgt gcagcacctc actggggtgc ttgacccggc tccaagagag
     1981 ctccgagaga tggacgaggc cgtcggcccc accgatgtcg acgaacgcgc cgaagtcggt
     2041 gatgctggtg acgcgcccac gccgcgtctc gccggcgtgc agctcctcga tcaggcgctc
     2101 cttcatcacg tcgcgccgct cctgcagcgc ctggcgctcc gagaggatga gccggttgcg
     2161 gtggcggttg atctcgatga tcttgagcga gagcttgctg ccgatcaagc gcgccatgtc
     2221 ggcctgcttg ctgccttcgt cgccgccgcg gatctcggtg acctgcgaag ccggcacgaa
     2281 gccgcgcacc ccgtcgaggt tcaccagcag gccacccttg ttgtagttgg tgacctcggc
     2341 ctcgatgacc tcaccggcgt cgtagacgtc ttgcagccgc cgccagctct tctccgccag
     2401 cgcccggtcg atcgagagaa ccgcctggcc ctcctgattc tccggctgca ccacgaagac
     2461 gagcacctga tcgccgaccg tcaaacgcct gcgctcctcc tcgttcaacg acgagaactc
     2521 gcgcgccggg atgacgccct ccgacttgga gccaatgtcg accagcagct cgtcgcggtc
     2581 gacgtgcatc agctgaccct ccagcacgtc cccgtagctc agcacccggt agtcgtgcaa
     2641 ggggtcgttg agaaactcct ccatgagcga caccggcagc tcgacgccgc cgtcatcggt
     2701 gctcacggtc tgttgcgtac gatccataca ccttcctcga tggtagattc acccaagaca
     2761 tctgtggcac ggtgcgaccc actgcctgca agacgtgctg ggcgttagca gaattatagc
     2821 cgctgccggc aaggcccggc aacccggcac gccgctcgcc atgacgattt tccctcttgg
     2881 aatcagtgat ttcacccaaa cctacctctt cgggcaggtg ccgcaccgct gtgcccgttt
     2941 cgtggcgaac gacgacattg agttccggtg aggacggatc cttcgactcc gctcaggatg
     3001 acagggaggg gaagcggcct accggacgag gaagatcgtg gtcgtccgcc ccatccctca
     3061 cccggtcatc ctgagcggag tcgaaggatc cgtacctcac gcggcaccca acgtcgtcga
     3121 ccgccgccca gccgctcaat acggcagctc cacgatctcg gcgtcgatct cctggctgat
     3181 gcgcagcatg ccgagcgtgt gccgcggctg ccagcgccgc tgcagcgccg agccggggtt
     3241 gaagaggagc aggccgtcca cgcgctggca gagcggccgg tggctgtggc cgaagatgcc
     3301 caggtcgacc tgacccgcga agatcgactc ggccgcctgg cgcgcggtca gccgtccgaa
     3361 gccgtcgccg tggatcatgc ccgccgagaa gaggccaaac tggaagcgtc gcgcgcgcgg
     3421 cagcagatcg cgcagcgcca tcgaatcatt gttccccacc accgcccgca gcggcggccc
     3481 gatctcggcg aagagcgcca gggcggccgc gctgcagaca tcgcccgcgt gcaggatcag
     3541 atcgacgcgc cgcagctccc gcacgagcgc ctccggcagc gggcgcggct cggcgcccag
     3601 gtgcgtgtcg gcgaccacac cgatcttgag cggagtcgcg aagcgctcgc tttccgccgg
     3661 ctggagcact agcgcttgcg gagagcggtg gtgagcgtgc tgctcacgat acccgccacg
     3721 atgccgccga ccagcgcgcc caggtaggtg atctcgacgc cgccggccag ccggccgccc
     3781 aggtagaaga ccgcgccgga gacgatcaac gagaagagcc cgagcgtcag gcagctgagc
     3841 ggccaggtga tgaggcgcag gatcggcgcg aggatcagat cgagcagcgt cagcaccgcc
     3901 gcgaaggcga tgatcgtctg ccaggtctgg tcgtagctca cctggcgcgg catgaaacgc
     3961 gccagcagcc agagcgtcag cccgttggcc accagcaggc ccacgatcct agccatcgac
     4021 gcctcccggc tccatgacgg catcccaccg ctcagcgatc ggcatcgtcg cgagcgccaa
     4081 gatcagcccg caggtcatcc agaacagcgc ggccatgtgc ggaaacatga tgttgaagta
     4141 gtagtggtca aacacgccca ccaccagtgc cgaggccagc gccgccagca ggccgagcaa
     4201 gaggtcgttg tacgcgccct cgggtgcctc gcgccaggcg cgccagcccg ccagcacgac
     4261 cgcgccgagc gcgaagagga agaccgccac cccgaggaac cccgcgcgct cggcgatcga
     4321 gaggtagatg ctggagacgc cggtctgctg gtcgatcgag ggcgccgcgc cgaagccgac
     4381 cccgaagagc gggtgcgtgc ggatgatcga cagcgcgttc tgatactcct gcagccgcag
     4441 cttggtggct ggatcctgca gcgtcagccc cagccagaaa cgatg
//
LOCUS       DASUBI010000339         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1118177, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000339 DASUBI010000000
VERSION     DASUBI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118177"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFU72_06545"
     CDS             <1..372
                     /locus_tag="VFU72_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423179.1"
                     /translation="GALGPDPQRLPDGVRHLRGYLFARFPRLLAIEATRRPAPGELEA
                     ALGEAGFAAIGTGTLWETRRRYPSFDDLAAELRARAGRSILHELSDDELAELIAFLRQ
                     RLPQSEPLIDRDRWTIWSGRR"
     gene            complement(277..1515)
                     /locus_tag="VFU72_06550"
     CDS             complement(277..1515)
                     /locus_tag="VFU72_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5423180.1"
                     /translation="MRLSLVISSLACGGSERVLSTIANFFAEPCRGWQVTLLTFDSGE
                     EPFYRLHPAVEHHALAIQRLCGACPRGTHRNLCYLWKLRRALRRSRPDVILAFVDRSN
                     VATLLASRGLGVPVVISERVNPRHWPIGGPGWRVLRRLLYPRAAALVVQTRAAQSGYP
                     AWLRPLTRVIPNPVVPREGAEQVPCTRPDGQTVIAAGRLVPQKGFDVLLQAFARVAPC
                     RPGWRLVIWGEGPERPALEALRAELGLDGRASLPGVTPVLGRELRGADLFVLSSRFEG
                     FPNVLCEALASGLPAIASDCPDGPRELIRDGIDGLLVPRDDVASLAGALERLMGDPAL
                     RGRLSAATGAARERFALERVMGLWSNTLRGAAGRAGYPAGDHAGAAASATAAPDRPAV
                     AVDERLRLGQPLPQEGDQLR"
     gene            complement(1800..>2511)
                     /locus_tag="VFU72_06555"
     CDS             complement(1800..>2511)
                     /locus_tag="VFU72_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5423181.1"
                     /translation="YAPRRGVERVLDLLERYGVPGTFFLEGWNARKYASLAREIEARG
                     HEVGAHGWMHERWNELAPEVERDLIRRTTETLGEALGHMPRGWRAPGALLTPRTLEFL
                     VDAGYQYDASFGDDDIPYLIQVGPGRSEELVELPMEWQLDDAVYYAHPGTIRRAGEVA
                     QLWIEEFDAYKTHAGYFMLVCHPRYSGRPARIEALERLIHHIAGLPGIWFARCHEVAD
                     VARDASWTPHFPYPERAE"
BASE COUNT          394 a          902 c          881 g          334 t
ORIGIN      
        1 ggtgcgctgg gtccagaccc acagcgcctg ccagacgggg tccggcacct gcgcggctat
       61 ctcttcgcgc gcttcccccg cctgctggcg atcgaggcga cgcgccggcc cgcgccgggc
      121 gagctggaag ccgcgctggg cgaggccggc tttgctgcga tcggcaccgg cacgctctgg
      181 gagacgcgcc gccgctaccc cagcttcgat gacctggccg ccgagctgcg cgctcgcgct
      241 gggcgctcga ttctccacga actcagcgat gacgagctag cggagctgat cgccttcctg
      301 cggcagcggc tgccccaatc ggagccgctc atcgaccgcg accgctggac gatctggagc
      361 ggccgtcgct gatgccgccg caccggcatg atccccggca gggtatccgg cgcggcccgc
      421 ggcgccccgc aaggtattac tccacaggcc catgacgcgc tccagcgcga agcgctcgcg
      481 tgccgcgccg gtggcggcag acaggcggcc acgcagcgcg ggatctccca tcagccgctc
      541 caacgccccg gccagcgagg caacatcgtc gcgcggcacc agcaggccgt cgatgccgtc
      601 acggatcagt tcgcgcggtc catcggggca gtcgctggcg atcgccggca gtccactggc
      661 cagcgcctcg cagagcacgt tggggaagcc ctcgaagcgc gaggacagca cgaagaggtc
      721 ggccccgcgc agctcgcgcc ccagcaccgg cgtgacaccg ggcaggctcg cccgaccgtc
      781 gaggccgagc tcggcgcgca gcgcttccag cgcgggccgc tccggtccct cgccccagat
      841 caccaagcgc cagcccgggc ggcagggcgc gacgcgggcg aaggcttgca gcagcacgtc
      901 gaagcccttc tgcggcacca gtcgtccggc ggcgatcacc gtctggccgt ccggccgggt
      961 acacggaacc tgctcggcgc cctcgcgcgg cacgacgggg ttcgggatga cgcgcgtgag
     1021 cggccgcaac caggccgggt agccggactg cgcggcccgc gtctggacta ccagggcggc
     1081 ggcgcgcggg tagagcagcc ggcgcagcac gcgccagccg ggaccgccga tcggccagtg
     1141 gcgcgggttc acccgctccg agataaccac cggcacaccc agcccgcggc tggccagcag
     1201 cgtcgcgacg ttggatcgat cgacaaatgc gaggatgacg tcgggccggc tccggcggag
     1261 cgcccgccgc agcttccaca ggtagcacag attgcggtgg gtgccgcgcg ggcacgcgcc
     1321 gcacagccgc tggatggcga gcgcgtgatg ctctaccgct ggatgcaggc ggtagaacgg
     1381 ctcttcaccg gagtcgaagg tgagcagtgt cacctgccag ccccggcagg gttcggcgaa
     1441 gaaattggca atagtcgaca gcacccgctc cgagccaccg caggcgagcg acgatatcac
     1501 gaggctcaat cgcatctcgg ttctcccacc ccaggcacgc agaatcaggg ccgagccgga
     1561 ggtgagttgg agacctgaaa agacgctgtc gacgcctcgg ctatcggtac tgggcggcgc
     1621 ggacaccctt ctttcgccgg tcatgctgag gacgcagccg aagcgtctcc tctcagctcc
     1681 gagatcgctg ctattccacc cgtagaagag atccttcgcc gcggcgcaga gcctgccctg
     1741 agcctgtcga taggatgacg gggggaaggc tccggcaggc gtgacgacac cgcggctggc
     1801 tattccgcgc gctcggggta ggggaagtgg ggcgtccagg aggcgtcgcg tgcgacgtcg
     1861 gcgacttcgt ggcagcgggc gaaccagatg ccgggcagtc cggcgatatg gtggatcaga
     1921 cgctccagcg cctcgatgcg cgccgggcgg ccgctatagc gcgggtgaca gaccagcatg
     1981 aagtagccgg catgggtctt gtacgcgtcg aactcctcga tccagagctg ggccacctcg
     2041 cccgcgcgcc ggatagtgcc ggggtgggcg tagtagaccg cgtcgtcgag ctgccactcc
     2101 atcggcagct cgaccagctc ctcgctccgc cccgggccta cctgaatcag atacgggata
     2161 tcgtcgtcgc cgaaggacgc gtcgtactgg tagccagcat cgacgaggaa ctccagcgtg
     2221 cggggcgtca agagggcacc gggggcgcgc cagccacggg gcatgtgccc cagcgcctcg
     2281 cctagcgtct cggtggtgcg gcggatcagg tcgcgctcga cctcgggcgc cagctcgttc
     2341 cagcgctcgt gcatccagcc gtgcgcgcca acctcgtgcc cgcgtgcctc gatctcgcgc
     2401 gccagcgacg cgtacttgcg cgcgttccag ccctccagga agaaggttcc cggcaccccg
     2461 tagcgctcca gcagatcgag cacacgctcg acaccccggc gcggcgcgta g
//
LOCUS       DASUBI010000340         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_532307, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000340 DASUBI010000000
VERSION     DASUBI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532307"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1105
                     /locus_tag="VFU72_06560"
     CDS             107..1105
                     /locus_tag="VFU72_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU5423182.1"
                     /translation="MSQGTQHRLGREAIHTAWDNTIPPRLTIALGDTVVFDTLEASYG
                     HVGRLVAGGELACEPGLAALISEGVYPAAPDRSGGHPLTGPVAIDGAQPGDTLVVEIL
                     AIEPAPWGWTGCRPGGIGLLDDEFDTAARHLWDLRDGRTAIFKPGISLPMAPFCGVMG
                     VAPAEPGRHPTPPPARHGGNMDIRQLTTGSTLYLPVLVPGALFSVGDVHGAQGDGEVS
                     GTGIEMEARVTLRFDLQQGRTIRQPQFRTADANPGPRGPYVAATGHHPDLREAAREAL
                     RGALDLLEERGLTRTEAYILASACVNLSISQVVDAPNYTVSAFVPLSIFVDRAAGG"
     gene            complement(1278..2132)
                     /locus_tag="VFU72_06565"
     CDS             complement(1278..2132)
                     /locus_tag="VFU72_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEU5423183.1"
                     /translation="MSLGAAVLTFREGLEAALIVAILLGYLRKIERPDARRYVLGGVA
                     LALAVTLGFVAILQVIGAEFEDPAAAIYEGTTSLLAVAMVTDMTFWMARHGRSMKGEL
                     EGEMRAKLARGSVWGLCALAFITVIREGIETGLFLSAATFASSGIDTLAGGLVGLAAA
                     IAVAWAMYVSGARLNLGKFFRIAGLLLVVFGAAILRYGVHEFEEVGVIPPLIEHVWNI
                     QATLPEKSGIGAVLQALVGYTAKPSLTQLIAYTGYFVVVGLALWKPWRSQPPRPRPNA
                     QVSITGSD"
BASE COUNT          393 a          910 c          832 g          361 t
ORIGIN      
        1 accgccattc gtccacgctt cccttcgaag gtcccgttca caacctccca gcaggccgta
       61 tcatgccgct gatatttcgc cggagcaggt aggggaggga gcgggcatga gccaggggac
      121 gcagcaccga ctgggccgcg aggcgatcca caccgcctgg gacaacacca tcccgccgcg
      181 gctaaccatc gcgctgggcg acacggtcgt cttcgacacg ctggaggcct cctacggaca
      241 cgtcgggcgg ctggtggccg gcggcgagct ggcctgtgag ccgggtctcg ccgcgctgat
      301 cagcgagggc gtctatcccg ccgcgcccga ccgcagcggc ggccacccgc tgaccggacc
      361 ggtggcgatc gacggcgcgc agccgggcga cacgctggtg gtcgagatcc tcgccatcga
      421 gccggcgccc tggggctgga ccggctgtcg ccccggcggc atcggcctgc tcgacgacga
      481 gttcgacaca gccgcgcgcc acctctggga cctgcgcgac ggccgcaccg ccatcttcaa
      541 gccgggcatc agcctgccca tggcgccctt ctgcggcgtg atgggcgtgg cccccgccga
      601 gccgggccgc caccccaccc cgcccccggc gcgccacggc ggcaatatgg acatccgcca
      661 gctcacgacc gggtccacgc tctacctgcc ggtgctggtc cccggcgcgc tcttctcggt
      721 gggcgacgtc cacggcgcgc agggcgacgg cgaggtcagc ggcaccggga tcgagatgga
      781 ggcccgcgtc acgctgcgct tcgatctcca gcagggccga actatccgcc agccgcagtt
      841 ccgcacggcg gacgcgaatc cggggccgcg gggaccgtac gtcgccgcca ccggccacca
      901 tcccgacctg agggaggcgg cgcgcgaggc gctacgcggc gcgctcgatc tgttggagga
      961 gcgcgggctg acccgaaccg aggcgtatat cctcgccagc gcctgcgtca acctctcgat
     1021 cagccaggtg gtcgacgcgc ccaactacac cgtcagcgcc ttcgtcccgc tcagcatctt
     1081 cgtggatcgg gccgccggcg ggtgatgcgg ggtggcggcg cggttgtcgg cggagcctgt
     1141 gcgctggggc cgcgccgggt ttcccggtcc gcgccagaga acgcaccaac ccggacgtag
     1201 ccaacgccga gaccccggcg aatgccggag tctctcacct cagatagtcg gagctgtgcg
     1261 cggctggcta cgtgttctca gtcagagcca gtgatgctca cctgggcatt tggacgcggg
     1321 cggggtggct gggaacgcca gggcttccag agcgccaggc cgacgaccac gaagtagccg
     1381 gtgtaggcga tcaactgcgt cagcgagggc ttcgcggtgt agcccaccag cgcctgcaag
     1441 accgcgccga tgccgctctt ctccggtaag gtcgcctgga tgttccaaac gtgctcgatc
     1501 agcggcggaa tcacgccgac ctcttcgaac tcgtgcaccc catagcgcag gatggccgcg
     1561 ccgaagacca ccagcaagag cccggcgatg cggaagaact tgcccaggtt gagccgcgcg
     1621 ccgctgacgt acatcgccca ggccacggcg atcgccgccg ccaggcccac gagcccgccc
     1681 gccagcgtat cgatccccga agaggcgaag gtggccgccg agagaaagag accggtctcg
     1741 atgccctcgc ggatcacggt gatgaaggcg agcgcgcaga gtccccagac gctaccgcgc
     1801 gccagcttgg cgcgcatctc gccctccaac tcgcccttca tcgagcggcc gtgccgcgcc
     1861 atccagaagg tcatatccgt caccatcgcg accgccagca ggctggtagt gccttcgtag
     1921 atcgccgcgg ccgggtcttc aaactcggcg ccgatgacct gcaggatcgc gacgaacccc
     1981 agcgtcaccg ccagtgccag cgccaccccc ccaagcacat agcgccgcgc gtcgggacgt
     2041 tcgatcttgc gcaggtagcc cagcaagatc gcgacgatca gggcggcttc cagcccctca
     2101 cggaacgtca gcaccgctgc acccaggctc atccagttct ccctctcgct ctccgactca
     2161 acgtttcgcg ttgagatgcc tgccggctgt aatcccgact ccgtggctcg gatatagctg
     2221 gagaataccg gttcgcgttc cccgctgtca accttctccc ggggctttgg ctggaaccgc
     2281 ccacggtggg ctggattgac gcgccgccgc gacccgccta cagtcggcgc gttggctgag
     2341 cggacggcgc ggcagggaga ccgattgatg actgtactgc tgaccggcgc gaccgggttc
     2401 gtgccctcga acatcgtgct ggcgctggtg gaggctggcg ataccgtgct ggcgctggac
     2461 cagcgcccgc ccgaggaacg gctgctgcgc gagctg
//
LOCUS       DASUBI010000341         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_657609, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000341 DASUBI010000000
VERSION     DASUBI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657609"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFU72_06570"
     CDS             complement(<1..561)
                     /locus_tag="VFU72_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423184.1"
                     /translation="MGTLLLMNASEALQAPAVSPQAASASAVETHGLRKVYGDKVAVA
                     DLTIRVEPGEIFGFLGPNGAGKSTSIKMLVGLVHPSAGSARLLGRPLGDRGARARIGY
                     LPELFRFHDWLSGEEFLDLHGQLYGMSRAARRARIPEVLALVGLQQAGSQRIRTYSKG
                     MQQRVGLAQALLNDPRLVFLDEPTSAL"
     gene            complement(645..1796)
                     /locus_tag="VFU72_06575"
     CDS             complement(645..1796)
                     /locus_tag="VFU72_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423185.1"
                     /translation="MMPEQRCLSDGELRDLIERPGAPHAHLVRCPGCRARLERIRADA
                     GAAAAILASPAPAEIDPTQAYHRWRQRQATDTTPTMKGDLMHRFTRVQRAGLAGAVLA
                     AVMALALIANPVTTLADSFLNQFRIQHFTAITVPMDLVQGLGAQAQAMTPADKAAISG
                     ELQKLGSFSTTFNMQGATKVGSLAEAQQQFSGLDVPKSLGAFQGDAASPSAIYLGQAG
                     SASYTVNVAEADKLLQSMNLGTQGLPDPQATPTVTFKVDVNPSAVMVWQQNGQHLVVG
                     QTESPVLNVPDSVNMDQLRDDLMQFPLIPADIKAQLNSVTDWKNTLIVPVPQGASHSD
                     VTIGNSQGLLVQSDKLSFVLWQDHGVLHVVASDSGANVVDIAKSLKPAA"
     gene            complement(1786..2397)
                     /locus_tag="VFU72_06580"
     CDS             complement(1786..2397)
                     /locus_tag="VFU72_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423186.1"
                     /translation="MATAKTLLAGSDAVPAETRRRESADVEDGCFEERFRRHYPTVYG
                     VLLRLTGNPEEAEDLAQEVFLRLHQHQAELDDERLAGWLYRVALNTGFNAVRSRRRTL
                     QRLLRWARLEPAQQHGLPGPEEEVMARDEARRVRQALTGLRQRDQAVLVLRYSGVSYA
                     EIAEATGIKPGSVGTILARAERALRERYVALTPGSEEESAHDA"
     gene            complement(2481..3581)
                     /locus_tag="VFU72_06585"
     CDS             complement(2481..3581)
                     /locus_tag="VFU72_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5423187.1"
                     /translation="MKRQIAFDSALGLIVAALVAGLIAGVALGTAAGSWLAGRPAASV
                     AGRGQTIALTQDGNAAVARVNPAVVTVDNLQRGGAGSEQVAGVGSGFIIDAQGHIVTN
                     HHVVAGADALAVIFSDGRKAPATLVGSDQFQDVAVLAVGGGVPAVAVFGDSDQLQPGQ
                     PVIAIGSALGEFHNTVTEGVVSGGGRSLDTGDGYRLEHLVQHDAPITPGDSGGPLIDA
                     SGKVVAMNTAVVTGGVAPSTAANLSFAIEANTVRGLAAQLIASGHVDRPYLGVAIQPA
                     RQAAAAPEDMHDSEPMPVVVVQVDQGSPAARAGLADGDQITAVAGTKLDEQHQFLNLV
                     YRHQPGETLTLQVRRDGQSRDVPVTLGQRTGA"
     gene            3710..>3921
                     /locus_tag="VFU72_06590"
     CDS             3710..>3921
                     /locus_tag="VFU72_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423188.1"
                     /translation="MEAPTPSPQPAEQPQPHPQPQRGHRVWRTLLIVGAALVIFGSST
                     GVGLVVDELFLQPGNAASASSVPSAF"
BASE COUNT          546 a         1401 c         1390 g          584 t
ORIGIN      
        1 gagcgccgag gtcggctcgt cgaggaagac caggcggggg tcgttgagca gcgcctgggc
       61 cagccccacc cgctgctgca tccccttgga gtaggtgcga atccgctggc tcccggcctg
      121 ctgcaaaccc accagcgcca gcacctctgg gatgcgcgcc cgtcgcgcgg cgcgggacat
      181 gccgtagagc tggccatgca ggtcgaggaa ctcctcgccg ctgagccagt cgtggaagcg
      241 aaagagctcc ggcaggtagc cgatgcgcgc ccgcgccccg cggtcgccga gcggacggcc
      301 cagcaagcgt gcgctgccgg cgctggggtg gaccaggccc accagcatct tgatcgaggt
      361 cgatttaccc gcgccgttcg gaccgagaaa gccgaaaatc tcgcccggct cgacccggat
      421 ggtcaggtcg gccaccgcga ccttgtcgcc gtagaccttg cgcagcccgt gcgtctcgac
      481 ggccgacgcg ctggcggcct gtggcgagac ggcgggcgcc tgcagcgctt cagaggcgtt
      541 catgaggaga agtgtgccca acagggacac gccccggcca gtcggccggg gcgtgtcggt
      601 gagggtggag gcgagccgcg acgggggaag gcacgcggcc acccctacgc ggcgggcttg
      661 agggacttgg cgatatcgac cacgttcgcg ccgctgtcgc tggccacgac gtgcagcacg
      721 ccgtggtcct gccagagcac gaacgagagc ttgtcgctct ggaccagcag cccctgagag
      781 ttgccgatcg tcacgtcgga gtgcgaggcg ccctgcggca ccggcacgat caacgtgttc
      841 ttccagtcgg tgacgctgtt gagctgggcc ttgatatcgg ccgggatcag cgggaactgc
      901 atcagatcgt cgcggagctg atccatgttg accgagtcag gcacgttgag caccgggctc
      961 tcggtctgac cgacgaccag gtgctggccg ttctgttgcc agaccatcac cgccgacggg
     1021 ttgacgtcga ccttgaaggt gaccgtcggg gtcgcctgcg gatcgggcag accctgggtg
     1081 ccgaggttca tgctctgcag gagcttgtcg gcctcggcca cgttcacggt gtagctggcc
     1141 gagccggcct ggcccagata gatcgcggac gggctggccg cgtcaccctg gaaggcgccg
     1201 agcgacttcg gcacatccag gccgctgaac tgctgctggg cctcggcgag cgagccgacc
     1261 ttggtggcgc cctgcatgtt gaaggtggtc gagaaactgc cgagcttctg cagctcgccg
     1321 ctgatggcgg ccttgtccgc cggggtcatc gcctgcgcct gcgcgcccag cccctgcacc
     1381 agatccatcg ggacggtgat ggcggtgaag tgctggatgc ggaactggtt caggaagcta
     1441 tcggccaggg tggtgaccgg gttggcgatc agcgccaggg ccatcaccgc ggccagcacg
     1501 gcgccggcca ggccggcgcg ttggacgcgt gtaaagcggt gcatcaggtc tcccttcatc
     1561 gtcggtgtcg tatcggtcgc ttgccgctgc cgccagcggt ggtatgcctg cgtgggatcg
     1621 atctccgccg gcgccggcga ggccaggatc gcagccgccg cgccagcgtc ggcgcggatg
     1681 cgctccaggc gagcgcggca gccaggacag cgcaccaggt gcgcgtgcgg cgcgccgggc
     1741 cgctcgatca ggtcgcgcag ctcaccatcg ctcaggcagc gctgctcagg catcatgcgc
     1801 ggactcctct tccgacccgg gcgtcagtgc cacgtaccgc tcgcgcagcg cgcgttcggc
     1861 ccgtgccaga atggtgccga ccgagccggg cttgatcccg gtcgcctccg cgatctcggc
     1921 gtaggagacg ccggagtagc gcagcaccag gactgcctgg tcgcgctggc gcagaccggt
     1981 cagcgcctgg cgcacgcggc gcgcctcatc gcgcgccatc acctcttcct ccggaccggg
     2041 caggccgtgc tgctgggccg gctccagacg cgcccagcgc aggaggcgct gcagcgtgcg
     2101 gcgacgcgag cgcacggcgt tgaagccggt attcagcgcc acccggtaga gccagccggc
     2161 caggcgctcg tcgtccagct cggcctggtg ctgatgaagc cgcaggaaga cctcctgggc
     2221 cagatcctcg gcctcctcgg gattgccggt cagccgcagc agcacgccgt agaccgtcgg
     2281 gtagtggcgg cggaagcgct cctcgaagca gccgtcctcc acgtccgccg actccctgcg
     2341 gcgcgtctcg gccggcacgg cgtccgagcc ggccagcagc gtcttggcgg tcgccatcgg
     2401 catccttccc gcgcggtccg gcggcccggc cgcgctcacc tatacaacac cacgccccgg
     2461 gcctttgtga caggcgccgc tcaggcgccg gtccgctggc ccagcgtcac cggcacgtca
     2521 cggctctggc cgtcgcgccg cacctggagc gtgagcgtct cgcccggctg gtggcggtag
     2581 acgagattga gaaactgatg ctgctcgtcg agtttggtgc ccgccaccgc ggtgatctgg
     2641 tcgccgtcgg ccaggccggc gcgcgcggcc gggctgccct gatcgacctg gaccaccacc
     2701 accggcatcg gctcgctgtc gtgcatatct tcgggtgcgg ccgccgcctg ccgggcgggc
     2761 tgaatggcca cgccgaggta gggtcggtcg acgtgcccgc tggcgatcaa ctgggcggcc
     2821 aggccgcgca ccgtattggc ctcgatcgcg aacgagaggt tcgccgcggt cgacggggcc
     2881 acgccaccgg tgacgacggc ggtgttcatc gccaccacct tcccgctggc gtcgatcagc
     2941 gggccgcccg aatcgccggg cgtgatcggg gcgtcgtgct gcaccagatg ctccagccgg
     3001 tagccgtcgc cggtgtccag gctgcgcccg ccgccgctga ccacgccctc ggtgacggtg
     3061 ttgtggaact cgcccagcgc gctgccgatg gcgatgaccg gctggccggg ctggagctgg
     3121 tcggagtcgc cgaagaccgc caccgccggc acgccgccgc cgaccgccag cacggccacg
     3181 tcctggaact gatcggagcc gaccagcgtg gccggcgcct tgcgtccatc gctgaagatg
     3241 accgccagcg cgtcggcgcc ggcgaccacg tggtggttgg tgacgatgtg cccctgcgcg
     3301 tcgatgatga agccgctacc cacgccggcc acctgctcgc tgccggcgcc gccccgctgc
     3361 aggttgtcga cggtcacgac cgccgggttg acccgggcga cggccgcatt gccgtcctgg
     3421 gtcagcgcga tggtctgccc gcgcccggcc acgctcgcgg ccggccgccc ggccagccag
     3481 gagccggcgg cggtgcccag cgctacgccg gcgatcagtc cggccaccag ggcggccacg
     3541 atcaggccca gcgcggagtc gaacgcgatc tgacgtttca cccggttcgt ccttccgtcg
     3601 ccggagcccg ttcgcgcctc gctgccggaa cacacggtgg agcacgtata ctccaggaaa
     3661 ttctacgtcg gctcagcgcg gcacgccccg cctggttctg aggagcgtta tggaagcacc
     3721 gacaccctcc ccccagcccg ccgagcagcc tcaaccccac ccgcagccgc agcgtggcca
     3781 ccgggtgtgg cgcacgctgc tgatcgtggg tgcggcgctg gtcatcttcg gctccagcac
     3841 cggcgtcgga ctggtggtcg acgagctgtt tctccagccg ggcaacgcgg ccagcgccag
     3901 cagtgtgccc agcgccttcg a
//
LOCUS       DASUBI010000342         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1020949, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000342 DASUBI010000000
VERSION     DASUBI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020949"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFU72_06595"
     CDS             complement(<1..460)
                     /locus_tag="VFU72_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5423189.1"
                     /translation="MFEGFTLSMIELEDVALRVRHGGSGPPLLLLHGHPETHAMWHKV
                     APRLAAEFTVVAPDLRGYGGSSKPPTTPDHAPYSMRTKARDMVALMRVFGCERFFVAG
                     HDRGGRCAYRLALDHPERVAKLAVLDIIPTGDAFRLADMSFGLTFWMWFFL"
     gene            complement(908..1828)
                     /locus_tag="VFU72_06600"
     CDS             complement(908..1828)
                     /locus_tag="VFU72_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5423190.1"
                     /translation="MRRSLLLLAPRRLEWVGEPLTAPGEGELLVETLAGAISVGSELP
                     LYRGDERASRPTTYPRMTGYESLGVVRSAGSGVSSPAVGERVVATYGHRDAALVPAAK
                     AIPVPPGLPDELALLGILSCDVAKGIGKLAPRADEPVLISGAGTIGLLTLWVLRAQGV
                     RTVDVIEPLPERRVLAETLGAAHAWAPGELTGDDYAAGFECSSRDAAFARLQRALRPG
                     GRIGILADGNVEPLTLTPEFHARELAVVGSSDGLDYQAHARWFFPRVQDGRSGLERLF
                     DCHVTAAELPATFERLATGELRAVKVLVRY"
     gene            2256..3104
                     /locus_tag="VFU72_06605"
     CDS             2256..3104
                     /locus_tag="VFU72_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5423191.1"
                     /translation="MSRSQRRRQALLALGACLAVWLLSVASGMAASYEVQSGDTLSGI
                     AEAYGVPLSVLAAQNHIGDVNLVYAGQQLVVPGQAPPAGPPPASADVYHARTGDSLWS
                     IAALRQTTLAQLIAANPEIVDPNHIEVDQTINLPSSAAAPSQAADVPALLEHYAGVYG
                     LDPALVKAIAWQESGWRQSVVSPDGALGVMQLLPSTGTWLARDVVGQPLDVSGSADDN
                     VHGGVAFLRFLIDRTGSVQLAVAAYYQGPGSLQRDGMLPETQHYVDNVLAIRSYLLLY
                     GVPPGA"
BASE COUNT          519 a         1185 c         1221 g          476 t
ORIGIN      
        1 ccaggaagaa ccacatccag aaggtcaggc cgaagctcat gtcggccagg cggaaggcat
       61 cgccggtcgg gatgatgtcc agcacggcca gcttggcgac gcgctcgggg tgatccagcg
      121 cgagccggta ggcgcagcgg ccgccgcggt catgtccggc cacgaagaag cgctcgcagc
      181 cgaagacgcg catcagcgcg accatgtcgc gcgccttggt gcgcatcgaa tacggcgcgt
      241 ggtcgggggt cgtcggcggc ttggagctgc cgccgtagcc gcgcagatcc ggcgcgacga
      301 cggtgaactc cgccgccaga cgcggcgcga ccttgtgcca catggcgtgc gtctcgggat
      361 ggccgtgcaa cagcagcagg ggcggcccgc tgccgccgtg ccggactcgc agcgccacgt
      421 cttccagctc gatcatggac aacgtgaatc cttcgaacat ctccggcacc cttctccatc
      481 tcatcgcgta cgagcgacgg ccgcgcgctg cgcccgcgag aggcagagca tactcctcag
      541 actttgccgc gcatgggcca ggtaccaccg ggcatgaccg acgacgatct cgtcttccct
      601 ctgaaacgga ggatcggtag cccggcgccg caggctgcct gacgcgagac ggccggccga
      661 cgggatcgag ccgggaggca ggcgcgagtc acgcagcggg tggcggtgat ggggtttcag
      721 ccgtcgcggg cagcttggcc ttccgactgg cgtaggccgg ggccagggtc cggctgatcg
      781 accgcgcgag tcggtggagg caccaccggc gccggccgct ggccggggaa ctcggccggg
      841 ccgcgctcgt cgaccaacgg tcagccggcc tgcaggtgcg cgaggaccgg gtcgcggaag
      901 gcgcggctca gtagcgcacc agcaccttga ccgcgcgcag ctcgccggtc gccaggcgct
      961 cgaaggtggc gggcagctcg gcggcagtga cgtgacaatc gaagaggcgc tccagccccg
     1021 agcggccgtc ctggacgcgg gggaagaacc agcgcgcgtg cgcctggtag tcgagtccgt
     1081 cgctggagcc gaccacggca agctcgcgcg cgtggaactc gggtgtgagg gtcagcggct
     1141 cgacgttgcc gtcggccagt atgccgatgc ggccgccagg gcgcagcgcc cgctgcagcc
     1201 gggcgaaggc ggcgtcgcgg ctggagcatt cgaagccggc ggcgtagtcg tcgccggtca
     1261 gctcgccggg cgcccaggca tgcgccgcgc ccagcgtctc ggccagcacc cgccgctcgg
     1321 gcagcggctc gatcacgtcg accgtgcgta ccccctgggc gcgcagcacc cagagcgtca
     1381 gcaggccgat cgtgccggcg ccgctgatca gcaccggctc gtcggcccgg ggcgcgagct
     1441 tgccgatccc cttagccacg tcgcagctga ggatgccgag gagcgccagc tcgtccggca
     1501 gcccgggcgg aaccgggatc gccttggccg ctgggacgag ggccgcgtcg cggtgcccgt
     1561 aggtggcgac gacgcgctcg ccgaccgcgg gcgaggagac accggaaccc gccgagcgca
     1621 ccacacccag gctctcgtag ccggtcatgc gcgggtaggt cgtcgggcgg ctggcccgct
     1681 cgtcgccgcg gtagagcggc agctcgctgc ccacgctgat cgcgccggcc agtgtctcga
     1741 ccagtagctc accttcgccc ggagccgtca gcggctcccc gacccactcc aaccggcgcg
     1801 gagccagcag cagcagcgag cggcgcacgc ctcactcccc ttcgacaggc gccgcggcat
     1861 ccacgcccgg ctcacccaaa agtaccggtc cgcgtgctca caagcgtaca cgctgacagg
     1921 cgggcatggg gcgcggggca agcctgatat ctgtccgccc cccatcgccg tcgcgcggag
     1981 tgaagtcggg cggccccggg agacacctct actgggatac gtaccgtgcc tgccgcggtc
     2041 gccttcgccg ggcccggtcg accgcctgtc aggtacgccg tgtcgctctg gcggccgtgc
     2101 gtgggtcacg gccggcgcca ggttctccgc ggtgacttgt ggtcaagttg acaggctgac
     2161 aaatttgaca tggggcgagc ggctgagtac agtgtgaacg tccgtcgcgt acggacagtg
     2221 agggagtagc cgtgcgaacg aagagagcag ggagcatgtc tcgatcgcag cgccggcgcc
     2281 aggcgctctt ggccctgggc gcctgcctgg ccgtctggtt gctgagcgtc gcctcgggca
     2341 tggcggccag ctacgaggtg cagagcggcg atacgttgag cggaatcgcg gaggcctacg
     2401 gcgtgccgct cagcgtgctg gcggcgcaga accacatcgg tgacgtcaat ctggtctacg
     2461 ccggtcagca actcgtcgtt cccggccagg cgcccccggc cggaccacct ccggcctccg
     2521 cggatgtcta ccatgcgcgg accggcgatt cgctctggtc gatcgccgcg ctgcgtcaaa
     2581 cgacgctggc gcagttgatc gcagccaacc ccgagatcgt cgatccgaat cacatcgagg
     2641 tcgatcagac gatcaacctg ccctccagcg cggccgcgcc gtcgcaagca gcggacgtgc
     2701 ccgcgctgct ggagcactac gccggcgtct acgggctcga tcccgcgttg gtcaaggcca
     2761 tcgcctggca ggagagcggc tggcggcaga gcgtggtgag cccggacggc gcgctagggg
     2821 tgatgcagct gttgccgagc acgggcacct ggctggcgcg ggatgtcgtc ggccagccgc
     2881 tcgacgtgtc cggcagtgcc gacgacaacg tccatggcgg cgtggccttc ctgcgcttcc
     2941 tgatcgatcg caccggctcg gtgcagctgg ccgtggcggc ctattaccag gggcccggca
     3001 gcctgcagcg cgacggcatg ctgcccgaga cgcagcacta cgtcgacaac gtgctggcga
     3061 tccgctcgta cctgctgctc tacggcgtcc cgccgggcgc ctagggcgat ccaaggcagg
     3121 accggtcacg gcctgggagg tcgcgaccgg tccgccggtt cccccgcacg tcatccagac
     3181 ctattccggt cgtcgactca tccggacggc atcaacgccg agccggagta cggcgaggtt
     3241 ccgcaagtac gtatctcccg caatattgcg caccagatga gcagctttgg ggctggaacg
     3301 cacgcggcgc gcgtgctaat ctggcagcga gcagcatttc acgagccgca tgagcggcac
     3361 gtcagccgac tgaccggtcg gtgctcgctg tcgagccggg a
//
LOCUS       DASUBI010000343         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_604203, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000343 DASUBI010000000
VERSION     DASUBI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604203"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /gene="fusA"
                     /locus_tag="VFU72_06610"
     CDS             complement(<1..1562)
                     /gene="fusA"
                     /locus_tag="VFU72_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5423192.1"
                     /translation="MSSTATQRIENQATALQRTRNIGIIAHIDAGKTTTTERILFYTG
                     RIHRPGEVHEGAATMDWMIQERERGITITSAATTCYWKDHRINIIDTPGHVDFTVEVE
                     RSLRVLDGGVVVFDAVAGVEPQSETVWRQADKYRVPRFCFINKMDRTGANFWRCVEMI
                     VDRLKARPAVTQLPIGSEASFLGIVDLLQMKAFIYHDELGQNIEQVEIPAEMLDEATM
                     RRHELVELIAETSEDLMMRYLEDEELSESELQAGLRAATIAGELVPVLCGTALKNKGV
                     QLMLDAIIDYLPSPLDIPPIHGLNPKTGEEEERHTDSEAPFSALAFKIMSDPHVGRLA
                     YIRVYSGTLASGSYALNSTKDQRERIGRLLRMHANHREEVAEVSAGDICAVIGLKNTF
                     TGDTLCDGDHPILLEAITFPEPVISVAVEPKTRSDQDKMGMALARLAEEDPTFQMYTD
                     QDSGQTIIKGMGELHLDVIIDRMKREFKVEANVGKPQVAYKETITRPVRVEGRFVRQT
                     GGRGQYGHVWLEL"
     gene            complement(1612..2082)
                     /gene="rpsG"
                     /locus_tag="VFU72_06615"
     CDS             complement(1612..2082)
                     /gene="rpsG"
                     /locus_tag="VFU72_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5423193.1"
                     /translation="MPRRAKVERRVPPPDARYGSELVARFINKVMQRGKKGVAERIVY
                     DALGMIQERTGRDAVEVFQQAIHNATPIVEVKPRRVGGATYQVPVQIEPNRRLSLAIR
                     WLLGSARARSGHSMAEKLAAELMDAANNTGATIKRREDTHRMAEANRAFSHYRW"
     gene            complement(2368..2793)
                     /gene="rpsL"
                     /locus_tag="VFU72_06620"
     CDS             complement(2368..2793)
                     /gene="rpsL"
                     /locus_tag="VFU72_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5423194.1"
                     /translation="MPTINQLVRKGRKRVTKKTKAPALRFTNNALGRHAGRLRRDKGS
                     PQKRGVCTQVRTMTPKKPNSALRKIARVRLTNGLEVTAYIPGEGHNLQEHSVVLIRGG
                     RVRDLPGVRYHIIRGALDARGVENRRQGRSKYGTKKPKS"
     gene            complement(2966..>4409)
                     /locus_tag="VFU72_06625"
     CDS             complement(2966..>4409)
                     /locus_tag="VFU72_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5423195.1"
                     /translation="PVIDSETGEILIDRGVELDENLVRQILDRGIARVFVRTAMHCQA
                     EHGVCQRCYGRNLASGRLVEPGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITTG
                     LPRVEELFEAREPKGKAVLAQNAGVARVIEDEQGRRIVITSEEIVTDTLELPAGYVMV
                     IGDGDNVTVGQTLAEAPEGQDGQPVAATMDGQVFFEGGQFVIRHENHEVRDYVISAGA
                     HIRIGDSDVVETGTQLTDGNLDPQEILETLGPDAVRSYIVDDVQKVYKSQGVVTNDKH
                     IEVIVRQMLRKVAVEHPGDTDLLVGELVDRFTFNRVNQDVIVQGGEPATAHQVLLGIT
                     KASLATDSFLSAASFQETTRVLTEAAIHGKIDYLRGLKENVIIGKLIPAGSGFNARLQ
                     AGGLTLSALDEAVLGTMSDEREQSERQQMEELVELMTGEQPVLAQPLAAPESSDEPAI
                     PPASLAAGALDDDADDDESIEDEPDEDEDA"
BASE COUNT          692 a         1518 c         1357 g          842 t
ORIGIN      
        1 agctccagcc agacgtggcc gtactgaccg cggccgccgg tctggcgcac gaagcggccc
       61 tcgacgcgca ccgggcgcgt aatcgtctcc ttgtaggcca cctggggctt gccgacgttg
      121 gcctcgacct tgaactcgcg cttcatgcgg tcgatgatca cgtccaggtg cagctcgccc
      181 atccccttga tgatggtctg cccgctgtcc tggtcggtgt acatctggaa ggtcgggtcc
      241 tcctcggcca gccgcgccag ggccataccc atcttgtcct ggtccgagcg cgtcttcggc
      301 tcgaccgcga ccgagatcac cggctccggg aaggtgatcg cctccagcag aatcgggtgg
      361 tcgccgtcgc agagcgtgtc gccggtgaag gtgttcttca gcccaatcac agcgcagata
      421 tcacccgcac tgacctccgc gacctcttca cgatggttgg cgtgcatgcg cagcagccgc
      481 ccgatacgct cgcgctgatc cttggtcgag ttgagcgcgt acgagccact ggccagcgtg
      541 ccggagtaga cccggatgta ggcgagacgc ccaacgtgcg gatcggacat gatcttgaag
      601 gccagcgccg agaagggagc ttcagagtcg gtatgccgtt cttcttcctc acccgtcttc
      661 gggttcagac cgtggatcgg cggaatatca agcggggagg ggagatagtc gatgatcgcg
      721 tccagcatga gctggacgcc cttgttcttg agcgcggtgc cgcagagaac cgggaccaac
      781 tcgcccgcga tggtggcggc gcgcaacccc gcctgcagct cagactcact cagctcctcg
      841 tcttcgaggt agcgcatcat cagatcttcg ctcgtctcgg cgatcagctc caccagttcg
      901 tgccggcgca tggtcgcctc gtcgagcatc tcggccggga tctcgacctg ctcgatattc
      961 tggccaagct catcatggta gatgaaggcc ttcatctgca ggagatcgac gatgccgagg
     1021 aagctcgcct cgctgccgat cgggagctgc gtgaccgccg gcctggcctt gagccggtcg
     1081 acgatcatct cgacgcagcg ccagaagttg gcgccggtgc ggtccatctt gttgatgaag
     1141 cagaagcgag gcacccgata cttatcggcc tgacgccaga cggtctccga ttgcggctcg
     1201 acaccagcca ctgcgtcgaa gacgaccacg ccgccgtcga gcacgcgcag cgagcgctcg
     1261 acctcgacgg tgaaatccac gtggccgggg gtgtcgatga tgttgatgcg atggtctttc
     1321 cagtagcatg tcgtggccgc cgaggtaatg gtgatgccgc gctctcgctc ctggatcatc
     1381 cagtccatcg tcgcggcgcc ctcgtgtacc tcgccgggcc ggtgaatgcg gcccgtgtag
     1441 aagaggatgc gctcggtcgt cgtcgtcttg ccggcatcga tgtgcgcgat gatgccgata
     1501 ttcctggtgc gctgaagcgc cgtggcttga ttttcgatac gctgggttgc agtgctgctc
     1561 atagtcgtcg tgattcctca atgtcccagc gcggaagcca ggactgcgcg cttaccatcg
     1621 ataatgcgag aacgcccggt tggcctcggc catgcggtgc gtgtcttcgc gccgcttgat
     1681 ggtcgcgccg gtgttattgg ccgcgtccat caattccgcc gcgagcttct cggccatgct
     1741 gtggccgctg cgcgcgcgcg cggatccgag gagccagcgg atcgccagag agaggcgccg
     1801 attgggctcg atctgaaccg gaacctggta ggtggcgccg ccgacgcgcc gcggcttgac
     1861 ctcgacgatc ggcgtggcgt tgtgaatcgc ctgctgaaag acctccaccg cgtcccggcc
     1921 ggtccgctcc tgaatcattc ccagcgcgtc atagacgatg cgctcggcaa cgcccttctt
     1981 gccacgctgc atgaccttat tgatgaagcg ggccaccagc tcgctcccgt accgggcatc
     2041 cggcggcgga acgcgccgct cgaccttcgc tcgcctcggc attgctctct tgcctccctg
     2101 taccagtctc cacaggcgcc cgcgcacccg gtcatttcgt ccggccgccg gcgccgtgcc
     2161 cgtcccgctc gtctcttcgc agcggcgact cgacgtcacc ctctccacga cacgaaaaag
     2221 cggtcagcac gaacgtaaac gtcgtgaggc cgctttccgt accgtacgat ctgcgaagag
     2281 aggctgcgag gacgggctca gctcaaccgg gggccgggct ctcaggggcg gctccgcgat
     2341 tcgtgagcca cccgtcgtcc gaaaccgcta actcttcggc tttttggtcc cgtacttcga
     2401 ccgcccttgc cggcggttct ctacaccgcg cgcgtcgaga gcgccccgga tgatgtgata
     2461 gcgcacaccc ggaaggtccc tgacacggcc accgcgaatg agcacgaccg agtgctcctg
     2521 caggttgtgg ccctcgcccg gaatgtatgc ggtgacttcc aaaccgttcg tcaaccgcac
     2581 acgggcgatc ttgcgcagcg ccgagttggg cttcttgggg gtcatagtgc gcacctgggt
     2641 gcaaacgccg cgcttctgcg gcgatccctt atcccggcgc aaacgaccag catgccggcc
     2701 aagcgcgtta ttcgtaaagc gcaacgccgg ggccttggtc ttcttggtga ccctcttccg
     2761 ccctttgcgg accaactgat tgatcgtcgg cacgtacgtc tctccttcta agacggccag
     2821 caaaaaccga accgtgctct gcctcggatg cagctgcggt gccgggcgtc ttttaaggaa
     2881 ggtcttcctc actcttgtgg ccgtccgtgg acggccacaa gcacctagtg tatcgcctac
     2941 gcccttacgt gtcaaggaaa aggcgctacg cgtcctcgtc ctcgtcgggc tcgtcctcga
     3001 tcgactcgtc gtcatccgcg tcatcgtcga gcgcgccagc cgcgaggctg gccggcggaa
     3061 tggccggctc gtccgacgac tccggcgcgg ccaggggctg cgccagcacc ggctgctcgc
     3121 cggtcatcaa ctcgaccaac tcttccatct gctggcgctc ggactgctca cgctcatcgg
     3181 acatcgtccc cagcaccgcc tcgtcgagtg cgctcagggt cagaccacca gcctgcaacc
     3241 gggcgttgaa gccggagccg gccgggatca gcttgccgat gatcacgttc tccttcagcc
     3301 cgcgcagata gtcgatcttg ccgtggatgg ccgcctccgt cagcacccgc gtcgtctcct
     3361 ggaaggaggc cgccgagagg aagctgtcgg tcgccagcga cgccttggtg atgcccagca
     3421 acacctgatg cgccgtggcc ggctcgcccc cctgcacaat cacatcctgg ttgacccggt
     3481 tgaaggtgaa gcgatccacc agctcgccga ccaaaaggtc ggtgtcgccg gggtgctcca
     3541 ccgcgacctt gcgcagcatc tgccgcacga tgacctcgat gtgcttgtcg ttggtgacca
     3601 cgccctgcga cttgtacacc ttctgcacat cgtccacgat gtaactacgc accgcgtccg
     3661 gccccaacgt ctccaggatc tcctggggat cgaggttgcc gtcggtcagt tgcgtgcccg
     3721 tctcgacgac atcgctgtcg ccgatccgga tgtgcgcgcc ggccgagatg acgtagtcgc
     3781 gcacctcatg gttctcgtgg cggatcacga actgaccgcc ctcgaagaag acctggccgt
     3841 ccatcgtcgc ggccaccggc tggccatcct ggccctccgg cgcctcggcg agggtctggc
     3901 cgacggtgac gttgtcgccg tcgccgatca ccatgacgta gcctgccggc agctccagcg
     3961 tatcggtcac gatctcttcg ctcgtgatca cgatgcgccg tccctgctca tcttcgatga
     4021 cgcgtgccac gccggcgttc tgcgccagga ccgccttgcc cttcggctcg cgcgcctcga
     4081 acagctcctc gacgcgcggc agaccggtcg tgatgtcctc gccggccaca ccgccggtgt
     4141 ggaaggtccg catcgtcaac tgggtgcccg gctcgccgat gctctgcgcg gcgatgatgc
     4201 cgaccgcctc gcccggctcg accaagcgac cgctggccag gttgcggcca tagcagcgct
     4261 ggcaaacacc gtgctcggcc tggcagtgca tcgcggtgcg cacgaagacg cgcgcgatac
     4321 cccggtccag gatctgccgc accagattct cgtccagctc gacgccgcgg tcgatcagaa
     4381 tctctccggt ctcggagtcg atcaccggg
//
LOCUS       DASUBI010000344         8401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_586094, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000344 DASUBI010000000
VERSION     DASUBI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586094"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VFU72_06630"
     CDS             <1..1044
                     /locus_tag="VFU72_06630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5423196.1"
                     /translation="GSSMLTATGRAVYALTRENLRLLREELPRELGDDFSLRLPGTLD
                     IATNQEQWEHLAESVAAQQAHGIDVRLLDRAELRAMVPVVSERVLGAKFAPGSGHLWP
                     FALVFALAAGARRHGATLATWTPVTRLLTANGAIVGVETAAGSVEAGAVVLATNAWTP
                     LLLPELPPGALVPARGQILVTQAMPGVLPHPFGTNFDKEYGRQTATGQILCGGFRRLD
                     VDEGLGHYEERVTAPVLGGIAGCLQTLFPPLGGSQWVRGWAGIMGFTADGLPLIGPYA
                     PLRGLFVAAGFNGGGFSWGAATGKALAELIVSGQSPFDLSPFDPARFHHAAITWANPF
                     TAGERNNPRRASG"
     gene            complement(1119..2789)
                     /locus_tag="VFU72_06635"
     CDS             complement(1119..2789)
                     /locus_tag="VFU72_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423197.1"
                     /translation="MKRSHWLLLAGLVVLTPGLGTPLTALGDPAPLSDRAPYPVPQLW
                     AQVDGPDTPSHAWVWGPTLLAAAHEPFAGAPGGTRQVFYFDKGRLQIDDPGQDPTSPG
                     YVGSGLLVRDLIAGEIQIGDDQYLTSTPANIPLTGDTVGNALAPTYAALNGVASIGDD
                     LANRRSPQRVGQPVTALMHADGSIDPNAVPQSDVSLATYSNATGHNIAKPFADWLSGQ
                     AQSWELLTGYPLTEPYWSDTVIAGKTRRVLFQAFERRVLTYTPGNPAGWQVESNNAGQ
                     HYRSWHHLATPADQPASWLADGVPFGEVIVRSARADNLDPFLVAALAQVASNFDPAYG
                     GPDGRAGLFGVRPQLLVGQATSLEPYVNAEAALRLLSDAHSHNSDWRAVLAIYYTGQA
                     HPDWTQPGLNTFVDGVLREQATLLSQFSQPPPPPAGPPAIVHRAAAIGAFANLYLVDS
                     GPAAYYSPNHTVAWWDYTVQRYKSWGQALPNAQPDPNGYYCVHPDFKVGDRLQLTANG
                     VTLWCTIGDTVAAQDEASWRAHWAVELSWNTFVALGLPGNNQVEVRHP"
     gene            complement(2975..4141)
                     /locus_tag="VFU72_06640"
     CDS             complement(2975..4141)
                     /locus_tag="VFU72_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5423198.1"
                     /translation="MTEREGGEYVETGGVEEAQAESPALLRAIERLSRAFAGHDVGGM
                     VEAIAAELADTPPLEAAGIWLDQSGCLTRRAWASGNERRADEQAVATAVAALRGVAYQ
                     QMLEQRSLLVLPLLRDEERLGALALVSTRPLKSVERTALTLLVRQVSAELAARSVDDS
                     PAAISEFIALVTHELRSPLTALRGNVQLAGMAARKGDLPRASERIEAALKGVDGITAL
                     LQNLQDISQLERGRFDLRLDPGELAPIVERAARRAERLTDLDQGVIALSTTSALVAQD
                     AGRLEQALFNLFVNALQYSRGSPVRVRLVVEGEEAVITIADRGIGIPREELGHIFEPY
                     YRGAAAAHGHAKGLGLGLTISRATVQRHGGTIEAESHAGQGNVFTIRLPLLAVA"
     gene            4276..4557
                     /locus_tag="VFU72_06645"
     CDS             4276..4557
                     /locus_tag="VFU72_06645"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU5423199.1"
                     /translation="MARLSDEELRAALAGLPDWARAGDAVEREFTFRTFRQAMAFANA
                     VAEIAHEARHHPDILIRYNRVRLTLSTHDEGGVTEKDIAFARAADAAYG"
     gene            4629..5612
                     /locus_tag="VFU72_06650"
     CDS             4629..5612
                     /locus_tag="VFU72_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU5423200.1"
                     /translation="MVTMSHWERVQAALDGLPVDHPPVSLWRHFPERDQTAQDLATVT
                     LAWQRRFGFDFVKLMPPGDYATIGWGAESEYQGSRGGTRQTTRYPISEIEDWRTISAV
                     PIDQGMNREVLEAARLVDEGLNGEVPVLQTIFSPLTIAMKLSNGQVIQHLRQEPALIH
                     DALRAITAVTRQLTEATLRHGASGIFFATQAASTDVMTPEEYVEFGVPYDILVLEAAL
                     TSRFTLVHIHGEHILFDELRTYPTQALNWHDRRTPPTLAEGQARSGRAVVGGIDERAI
                     VRMTPDAAATQARDAVAELNGRAMMVAPGCVIPIDTPEANVEAVVRAVRGA"
     gene            complement(5747..6220)
                     /gene="paaD"
                     /locus_tag="VFU72_06655"
     CDS             complement(5747..6220)
                     /gene="paaD"
                     /locus_tag="VFU72_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU5423201.1"
                     /translation="MTAISEAAIWERLREVPDPEIPTISVVDLGIIRSVEVGETIRVA
                     LLPTFVGCPAIDVMRRDIAAKLADLGEVEVKVVYDEAWTTQRITLHGRKMLKKAHLVP
                     PPPGDVAAEGDGEPVACPYCGSTETQLENLFGPTPCRSIYYCRHCRQPFERFKPV"
     gene            complement(6251..7066)
                     /gene="paaC"
                     /locus_tag="VFU72_06660"
     CDS             complement(6251..7066)
                     /gene="paaC"
                     /locus_tag="VFU72_06660"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU5423202.1"
                     /translation="MSREPDAQPQAALRRHLITLADDELVLGYRCSEWTGVAPMVEED
                     VAFSSLAQDEIGHARLYYTLASELGGGDPDRLALDRAPGDYYHARVLEQRTAPRYDPS
                     GLAPQGGDWARAVALCELYERFDELRTAVLAECSLPQIAGALDKVRREERYHLRHAAD
                     WWSRLAGGSPEARDRLQQAIDDLWPGVLGLFEGSPGEALLVEVGVLPGMTAALRPRWL
                     EALAPAFAAYGLTLPSAETAPLLGGRQGIHGPEWDALHDEMTMVRRLAPEGVW"
     gene            complement(7056..7346)
                     /locus_tag="VFU72_06665"
     CDS             complement(7056..7346)
                     /locus_tag="VFU72_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HEU5423203.1"
                     /translation="MQVFEVFRQEEEGEALRHAGSVDAPDEALAIQYAREVYSRRGEA
                     TRLWVVPRAAIVEVVDEDFLHPVIERSYRLGEGYRVTVAKRRQLKEAGAGEP"
     gene            complement(7372..8295)
                     /gene="paaA"
                     /locus_tag="VFU72_06670"
     CDS             complement(7372..8295)
                     /gene="paaA"
                     /locus_tag="VFU72_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU5423204.1"
                     /translation="MSVMSIEERTERFRRHVREGHQVEATDWLPDEYRARLTKFIEMH
                     ANSEMMGALPERDWILRAPTLHRKLAVTAKIQDEVGHAQLLYRLVEDLGKPREQIFDD
                     LIGGKTKFHNVFHYPTRSWGDVGIIAWLVDAAAIFSQSALQRSSYAPYARVMRRICWE
                     EAFHLKHGEDIVLTAMMGTPAQQALMQEALERWWEPLMMFHGPPTPVEQDKDLAWGIK
                     SRPNEELRQDFLTRYVRKIQEIGLKIPDSRLRFDEASGRWQYSEPDWDKLYWIVTGNG
                     PASRERLGFRRLSYGESDWVRQAVAGQAAAA"
BASE COUNT         1181 a         2982 c         2994 g         1244 t
ORIGIN      
        1 gggtcgtcga tgctgaccgc caccggtcgc gccgtctacg ccctgacccg cgagaacctg
       61 cgcctgctgc gcgaggagct gccgcgtgag ctgggcgacg atttctcgct gcgcctgccg
      121 ggcacgctcg acatcgccac gaaccaagag cagtgggagc acctggccga gagcgtcgcg
      181 gcccagcagg cgcacgggat cgacgtgcgg ctgctcgacc gcgccgagct gcgcgccatg
      241 gtgccggtcg tctccgagcg cgtgctgggc gccaagttcg cccccggcag cggccatctc
      301 tggccattcg ccctggtctt cgccctggcg gccggcgcgc gccgccacgg cgccacgctg
      361 gccacctgga cgccggtcac gcgcctgctg acggccaacg gcgcgatcgt gggagtcgag
      421 acggcggcgg ggagcgtcga ggctggcgcg gtggtgctgg ccaccaacgc ctggacaccg
      481 ctcctcctgc ccgagctgcc gcccggcgcg ctggtaccgg cgcgcggcca gatcctggtg
      541 acgcaggcga tgcccggcgt gctgccgcac cccttcggca ccaacttcga caaggagtac
      601 gggcggcaga cggccaccgg ccagatcctc tgcggcggct tccgccggct cgacgtcgac
      661 gagggcctgg ggcactacga ggagcgggtc accgcgccgg tgctcggcgg catcgccggc
      721 tgcctgcaga cgctcttccc gccgctgggc ggctcgcaat gggtgcgcgg ctgggccggc
      781 atcatgggct tcaccgccga cggtctgccc ctgatcggcc cctacgcgcc cctgcgcggg
      841 ctgttcgtcg ccgccggctt caacggcggc ggcttctcct ggggcgccgc caccggcaag
      901 gcgctggccg agctgatcgt cagcggccaa tctcccttcg acctctcgcc cttcgacccg
      961 gcgcgcttcc accacgccgc catcacctgg gccaacccct tcaccgccgg tgagcgcaac
     1021 aacccgcgac gggccagcgg atgagggcta ttcgccctgc ccgctggccc gggcattcgg
     1081 gattagccgc gggggccggc gatggcgccg gccctctcct acggatgccg tacctcgacc
     1141 tgattgttgc ccggcagccc gagcgccacg aacgtgttcc aggagagctc gaccgcccag
     1201 tgggcgcgcc aagaagcctc gtcctgtgcc gccaccgtgt cgccgatcgt gcaccagagc
     1261 gtgacgccat tggcggtcaa ctgcagccgg tcgcccacct tgaagtcggg gtggacgcag
     1321 tagtagccgt tggggtcggg ctgcgcgttg ggcagggcct gaccccagct cttgtagcgc
     1381 tgcaccgtgt agtcccacca ggccacggta tggttcggcg agtagtaggc cgccgggccg
     1441 ctatcgacca ggtacaggtt cgcgaacgcc ccgatggctg ccgcccggtg cacgatggcc
     1501 gggggaccgg ccggcggcgg aggcggctgg ctgaattgcg acagcagtgt cgcctgctca
     1561 cgcagcacgc cgtcgacgaa ggtgttgagg cccggctgcg tccagtcggg gtgcgcctgg
     1621 ccggtgtagt agatcgccag caccgcgcgc caatcgctgt tgtggctgtg cgcgtcggac
     1681 agcaagcgca acgcggcctc ggcgttgacg tacggctcga gcgacgtcgc ctggcccacc
     1741 agcagttgcg gccgcacgcc gaagagcccc gcgcggccat ccgggccgcc atacgccgga
     1801 tcgaagttgc tggcgacctg ggccagcgcg gctaccagga acggatcgag gttgtcggcc
     1861 cgcgccgagc ggacgatcac ctcgccgaag ggcacgccgt cggccagcca gctcgccggc
     1921 tggtcggccg gggtcgccag atgatgccag ctacggtagt gctgacccgc gttgttcgac
     1981 tcgacctgcc agcccgcggg gttacccggc gtgtaggtca gcacgcgccg ctcgaaagcc
     2041 tggaagagga cgcgccgggt cttgccggcg atgacggtgt cgctccagta gggctcggtc
     2101 agcgggtagc cggtcagcag ctcccacgac tgcgcctggc ccgacaacca gtcggcgaaa
     2161 ggtttggcga tgttgtggcc ggtggcgttg gagtaggtcg cgagcgacac gtcgctctgg
     2221 ggaaccgcgt tggggtcgat cgagccgtcg gcgtgcatca gggccgtcac cggctggccg
     2281 acgcgctgcg ggctgcgccg gttggcgagg tcgtcaccga tcgaggcgac gccattgagc
     2341 gccgcgtagg tcggcgccag cgcgttgccg accgtgtcgc cagtgagcgg gatgttggcc
     2401 ggggtgctgg tgaggtattg gtcgtcgccg atctggattt cgccggcgat caggtcgcgt
     2461 accagcagtc cgctgccgac gtagccgggg gaggtgggat cctggcccgg atcgtcgatc
     2521 tgcagccgtc ccttgtcgaa gtagaacacc tgacgcgtgc cgccgggcgc gccggcgaag
     2581 ggttcgtggg cggcggccag gagagtcggc ccccagaccc aggcatggct tggcgtatcc
     2641 ggtccgtcga cctgcgccca gagctgtggc accgggtacg gagcccggtc gctcagcggc
     2701 gccggatcgc ccagcgccgt cagcggcgtc cccaaccccg gcgtcagaac gaccagtccg
     2761 gccagcagca gccagtgtga tcgcttcaaa taccctcccc gcattggacc aatcgcgtcc
     2821 ggcggctgga cacggaatgg gtgcgggcca gccccggccg ggcccgatac cgggccatcg
     2881 tcatgctagg cagcggtggt tgcgcgaaca ataagttttg gtgggaatat cgtgatgtgc
     2941 ttggcaggca acgccggacc ggggtactcc gacgctaggc gacagccagc agcggcaggc
     3001 gtatggtgaa gacgttcccc tggccggcgt ggctctcggc ctcgatcgtg ccaccgtggc
     3061 gctgcaccgt ggcgcggctg atggtcagcc cgagcccgag ccccttggcg tggccgtgcg
     3121 ccgctgccgc tccccggtaa tagggctcga agatgtgccc cagttcctcg cgcgggatgc
     3181 cgataccccg gtcggcgatg gtgatgaccg cctcttcgcc ctcgaccacc agccgcacgc
     3241 gcacgggact tccacgtgaa tactggagcg cgttgacgaa caggttgaag agcgcctgct
     3301 ccagccggcc cgcgtcctgc gccaccagtg ccgaggttgt cgacagggcg atcacgccct
     3361 gatcgagatc ggtcaggcgc tcggcgcgcc gggcggcgcg ctcgacgatc ggcgccagct
     3421 cgcccggatc gagccggaga tcgaaccggc cacgctccag ctggctgatg tcctgcaggt
     3481 tctgcagcag ggcggtgatg ccgtcgaccc ccttgagggc ggcttcgatg cgctcgctgg
     3541 cgcggggcag atcgcccttg cgcgcggcca tgcccgctag ctgcacgttg ccgcgcaggg
     3601 cggtcagcgg cgagcgcagc tcgtgcgtga ccagcgcgat gaactcgctg atggccgccg
     3661 gcgagtcgtc caccgagcgg gcagccagtt cggcggacac ctggcgcacc aggagcgtga
     3721 gcgcggtccg ttcgacgctc ttgagcgggc gcgtgctgac gagggccagc gcacccaggc
     3781 gttcttcgtc gcgcagcagc gggagaacca gcagactgcg ctgctccaac atctgctggt
     3841 acgctacgcc gcgcagcgcg gcgaccgccg tggcgacggc ctgctcgtcg gcgcgcctct
     3901 cattgccgct ggcccaggct cgccgtgtca ggcagccgga ctggtcgagc cagatgccgg
     3961 ccgcctctag cggcggcgtg tccgccagct cggcggcgat ggcctcgacc atgcctccca
     4021 cgtcatgccc ggcgaacgcg cggctcaagc gctcgatcgc acggagcagc gccgggctct
     4081 ccgcctgggc ctcctcaacc ccaccggttt ccacgtactc tccgccctct cgctcggtca
     4141 tctcagcgcg cggccggtcc cagcgcgttc gctcccgccg cccgccgcac tgacccgtac
     4201 ttcgcaacac gggtgccagt aggcggactc ggtgtagatt gagtaggggc cgtttggtcc
     4261 tggaatggag gcgacatggc acgactatca gacgaggaac tgcgcgcggc gttggccggg
     4321 ctgccggact gggcgcgagc cggcgatgcc gtcgagcggg agttcacctt ccgcaccttt
     4381 cgccaggcga tggccttcgc caacgcggtg gccgagatcg cccacgaggc gcgccaccac
     4441 cccgacatcc tgatccgcta caaccgggtg cggctgacgc tgagcaccca cgacgagggc
     4501 ggtgtgaccg agaaggacat cgccttcgcg cgggccgcgg acgcggcgta tgggtgacgg
     4561 gcgcggcggg ccgatggtag tctgaggcgg acggctggcc cgtggccggc ggcgagagga
     4621 gtagtccaat ggttaccatg tcccactggg agcgggttca ggccgcgctc gacggcctgc
     4681 cggtcgatca tccgccggtc agcctgtggc gccacttccc ggagcgcgac cagaccgccc
     4741 aggatctggc gacggtcacg ctggcgtggc agcggcgctt cggcttcgac ttcgtcaagc
     4801 tgatgccgcc cggcgactac gccacgatcg gctggggcgc cgagagcgaa taccagggga
     4861 gccgcggcgg cacccgacag acgacacgct acccgatcag cgagatcgag gactggcgca
     4921 cgatcagcgc ggtgccgatc gaccagggga tgaaccgcga ggtgctggag gccgcccggc
     4981 tggtcgacga ggggctgaac ggcgaggtgc cggtgctgca gacgatcttc agcccgctga
     5041 ccatcgccat gaagctctcg aacggccagg tgatccagca cctgcggcag gagccggcgc
     5101 tgatccacga cgcgctacgc gccatcacgg ccgtcacgcg gcagctgacc gaggccaccc
     5161 tgcggcacgg cgcctcgggc atcttcttcg ccacccaggc cgccagcacc gacgtcatga
     5221 cgcccgagga gtatgtcgag ttcggcgtgc cctacgacat cctggtgctg gaggccgcgc
     5281 tcacctcgcg cttcacgctg gtccacatcc acggcgagca catcctgttc gacgagctgc
     5341 gcacctaccc cacccaggcg ctcaactggc acgaccggcg cacgccgccg acgctggccg
     5401 agggccaagc gcgcagcggc cgcgccgtcg tcggggggat cgacgagcgc gccatcgtgc
     5461 gcatgactcc ggacgccgca gcgacccagg cgcgcgacgc cgttgccgag ttgaacggcc
     5521 gcgccatgat ggtcgcgccc ggctgcgtca tcccgatcga cacgcccgag gccaacgtcg
     5581 aggccgtggt gcgggcggtg cgcggggcgt agcggtcgcg acgcgtgacg acgtgggcag
     5641 catccgtgaa gccgtcgtgc caggcgccgg gcggcacgag tgccccacgg aggacgtcca
     5701 acgtcgtcgt ggggcagatg gacaagatgc accgtggtcg cggcgctcac accggcttga
     5761 agcgctcgaa gggctggcgg cagtggcggc agtagtagat cgagcggcag ggcgtcggcc
     5821 caaagaggtt ctcgagctgc gtctcggtcg agccgcagta ggggcaggcg accggctctc
     5881 cgtcgccctc cgcggccacg tcgccgggcg gtggcggcac caggtgcgcc ttcttgagca
     5941 tcttgcggcc gtgcagcgtg atgcgctgcg tggtccaggc ctcgtcgtag acgaccttga
     6001 cctcgacctc gcccagatcg gccagcttgg cggcgatgtc gcgccgcatg acgtcgatgg
     6061 ccgggcagcc gacgaaggtc gggagtaatg cgacgcggat cgtctcgccg acctcgaccg
     6121 agcggatgat gccgagatcg accacgctga tggtcgggat ctcgggatcg ggcacctcgc
     6181 gcaggcgctc ccagatcgcg gcctcgctga tggcggtcat cttccgctcc tctccgggcg
     6241 ctcccagcgg ctaccagacg ccttcgggcg ccagccgtcg caccatcgtc atctcgtcgt
     6301 gcagcgcgtc ccactcaggg ccgtggatgc cttgccggcc gccgagcaag ggtgcggtct
     6361 ccgccgacgg cagcgtcagc ccataggcgg cgaacgcggg cgcgagcgcc tccagccagc
     6421 gcgggcgcag cgccgcggtc atccccggca ggacccccac ctcaaccagc agcgcctcgc
     6481 cgggcgaccc ctcgaagagg ccgagcacgc ccggccagag gtcgtcgatg gcctgctgga
     6541 gccggtcgcg cgcctcgggg gagccgccgg ccagccggct ccaccagtcg gcggcgtggc
     6601 gcaggtggta gcgctcctcg cgccgcactt tatccagcgc gcccgcgatc tggggcagcg
     6661 agcattcggc cagcacggcg gtgcgcagtt cgtcgaagcg ctcgtagagc tcgcacagcg
     6721 cgacggcccg ggcccaatcg ccgccctggg gcgccaggcc cgaggggtcg tagcgggggg
     6781 cggtgcgctg ctccagcacg cgcgcgtggt agtagtcacc gggcgcgcgg tcgagcgcca
     6841 ggcgatcggg gtcgccgcca cccagctcgc tcgccagcgt gtagtagagg cgcgcgtgtc
     6901 cgatctcgtc ctgcgccagc gagctgaagg cgacgtcctc ttccaccatg ggcgccacgc
     6961 cggtccactc cgagcagcga tagccgagca ccagctcgtc gtcggccagg gtgatcaggt
     7021 ggcggcgcag ggcggcctgg ggttgcgcgt ccggctcacg gctcaccggc gcccgcctcc
     7081 ttgagctgcc gccgcttggc caccgtcacc cggtagccct cgcccagccg gtagctgcgc
     7141 tcgatcaccg ggtgcaggaa gtcctcgtcg acgacctcga cgatggcggc gcgcggcacg
     7201 acccagaggc gcgtggcctc gccgcggcgc gagtagacct cgcgcgcgta ctggatggcg
     7261 agcgcctcgt cgggcgcgtc gacgctgccg gcgtgccgca gcgcctcgcc ctcctcctct
     7321 tgccggaaga cctcgaagac ctgcactggc tcgctcgctt tcccggccgc ctcaggcggc
     7381 cgcggcctgc cccgccaccg cctggcgcac ccagtcgctc tcgccgtagg agagccggcg
     7441 gaagccgagc cgctcccgcg aggccgggcc gttgccggtg acgatccagt agagcttgtc
     7501 ccagtccggc tcgctgtact gccagcggcc gctcgcctcg tcgaagcgca gcctggagtc
     7561 ggggatcttg agcccgatct cctggatctt gcgcacatag cgggtcagga agtcctggcg
     7621 cagctcctca ttcggccggc tcttgatgcc ccaggcgagg tccttgtcct gctcaaccgg
     7681 cgtcggcggg ccgtggaaca tcatcagggg ctcccaccag cgctcgagcg cctcctgcat
     7741 cagcgcttgc tgggccggcg tgcccatcat ggccgtcagc acgatgtcct cgccgtgctt
     7801 gaggtggaag gcctcctccc agcagatgcg gcgcatgacc cgggcgtagg gggcatagct
     7861 ggagcgctgc agggcgctct gactgaagat ggcggcggca tcgaccagcc aggcgatgat
     7921 gccgacgtcg ccccaggagc gcgtcgggta gtggaagacg ttgtggaact tggtcttacc
     7981 gccgatcagg tcgtcgaaga tctgctcgcg cggcttgccg agatcctcga ccaggcggta
     8041 gaggagctgg gcgtggccga cctcgtcctg gatcttggcg gtgacggcca gcttgcggtg
     8101 cagcgtcggc gcgcgcagga tccagtcgcg ctcggggagc gcgcccatca tctccgagtt
     8161 ggcgtgcatc tcgatgaact tggtcagccg ggcgcggtac tcgtcgggca gccagtcggt
     8221 cgcctcgacc tgatgcccct cccgcacatg ccgccggaag cgctcggttc gctcctcgat
     8281 cgacatcaca ctcatgctgc acctcctggc gggttgctgg ctcgattccc ggtactgccc
     8341 ggcgctggga cgtgagacac gccaatcata ccgctgcaag cgggctgcca ccgcgcggag
     8401 g
//
LOCUS       DASUBI010000345         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_805971, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000345 DASUBI010000000
VERSION     DASUBI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805971"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..349
                     /locus_tag="VFU72_06675"
     tRNA            274..349
                     /locus_tag="VFU72_06675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Gly,seq:tcc)
     gene            945..3545
                     /locus_tag="VFU72_06680"
     CDS             945..3545
                     /locus_tag="VFU72_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423205.1"
                     /translation="MESNSQELLGVLDAAIAKGVHQETVRRAIRAGHLPALRIGRNFV
                     IRPADLQGWRPHYEKDPGRREAGRTPAARPGRSATPPAMRLGVRSRLGAPPSHNVTVP
                     PTPLLGRERELEIVRTYLLSAGKRLVTLTGPGGAGKTRLALELAQQLVGSFEQGVWLV
                     DLVPLRDAALIISAIAQTLDIRDLGRRPLGEVLVAWLRHRRLLLVLDNCEHLLAGMSE
                     VATLLGACPGLRVLATSRTALHLAEEQVAPLHPLPVPDLARASSAGAVGQSPAVALFV
                     QRASAVDPTFQLTDVNAPTVAEVCVRLDGLPLAIELAAARARVLSVEQIAAHLDDRFH
                     LLTAGPRAALPRQQTLRATVDWSYALLSEPERALLRRLSVFAGGWTLEAAESVAAGDG
                     VQPSAVLDLLAQLVDESLVIAEARRGAMRYRLLETIREDARDRLQAVGDAERTRDRHL
                     AYYLALAEDAELKLRGAEYQLYLNRLEEEHANLRAALGWALASPDGGEASLRLSGALA
                     WYWWLRSHHDEGWRWLQRALAGTADTSAARMKALYGAGFLAHHRRDSTLARALLDESL
                     AIARARDDRWTVAWVLHHLGRVAYFDDDPAAARALGEESLAVAEAIGDRWLNAWPLHL
                     LGLAAHIAGDYSSAREYYARSLAIRRELGYQEGIGILLVLLGVVALREGDLARAHALF
                     REGVEGAQTVLGPWGLAMPLAGFAHLAAASGQPLRATRLGAAAAAQSEASHTPLIPLF
                     DALLREGLEAARRALDEGAYAAAWAEGRVLSLEESIAEALAVEAGPPTLPPAPAAHPR
                     RDGAFAQLTPAEFEVLRLLSGGRTTREIAAGLVVSISTVDRHLTHLYGKLGVRNRAEA
                     TAFALRHGLL"
     gene            3767..>3972
                     /locus_tag="VFU72_06685"
     CDS             3767..>3972
                     /locus_tag="VFU72_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU5423206.1"
                     /translation="MTTSRQIATTSVDDATIEAFRADFRGELSRPGDTTYDKTRSVFN
                     GMIDRHPALIARPTGPADVVAAVK"
BASE COUNT          594 a         1350 c         1377 g          651 t
ORIGIN      
        1 cacccagcag agcggctccg gcatggggct ggggctcggc ctgcatatct gcaagaccat
       61 cgtcgagcgg catggcggca aggtcggcgt ccgcagcgcg ccggaccagg gcagcgtctt
      121 ctggttcacg ctgcccctgg cggatgagac gccagagcga cctgcgccct agccactcta
      181 tctcgcggcc tcgctgctct atccgcgcac ggcgcgatgt ggtatgctat cgcctgttgg
      241 cgtccgggcg cgaagtctgg cgctggcgac cacgcgggag tagctcagtt ggtagagtac
      301 ctgccttcca agcagggcgt cgcgggttcg agtcccgtct cccgctccag acctggattt
      361 tccggggttt gtgctccttc cctgcaacga cacaggcagg gtgtcacctc aaacgtcgaa
      421 cccctcgccg cgcgtaaata actaggtaac atccgtcctg acgccgagcg attgccgcta
      481 actgcctgaa agcagagcgt cgtgtggccg cggccgcatg acgggctagc ggcattggat
      541 agatcccctc acccggcggt agaggtggct cctccccatc tcgagtgtcg tcacgctggc
      601 gtcgtcttgg tcactgaaat ttgactcaga tctgacgtga atgcccattg acttaccaag
      661 agcacgtggt gcgggcgcag cccatgggcg gcctctgaga catcgtgccg tctcgggtta
      721 gtgcagtata gatcacacgg acctccgccc gattcgtagg cgagggcgag agcgaggacg
      781 tggatggcca aaacagttgg taccgagcaa gagtgatgtc cgttctcgct ccgccggggc
      841 ctgacccagg cccgctgtcc ttgtagatct gttgcgtcga gatgacacat cgcgtacaat
      901 cagggcgtgt cgcgaggcca cgcatcgaca atcgcccaag aaccatggaa agcaatagcc
      961 aagagctact cggcgtcctc gacgctgcga ttgccaaggg tgtccaccag gagacggtgc
     1021 ggcgcgccat ccgcgctggc catctgcctg ccctgcggat cgggaggaac ttcgtcatcc
     1081 gccccgccga cttgcagggc tggcggcccc actatgagaa agaccccggg cgacgggaag
     1141 ctggccgcac cccggccgcg cgacccggac gatcggccac accgccggcc atgcggttgg
     1201 gcgttcggtc gcggctgggg gctcctccct cccacaacgt gaccgtgccg cccacgccgc
     1261 ttctcggccg cgagcgcgag ttggagatcg tacgcaccta tctgctgagc gcgggcaagc
     1321 ggctagttac gctgaccggt ccgggtggcg cgggcaagac gcgcctggcc ctggagcttg
     1381 cccagcagct cgtcggctcc ttcgagcagg gggtgtggct cgtcgacctg gtcccgctgc
     1441 gcgacgcggc gctgatcatc tcggccatcg cccagacgct ggacatccgc gatctcggtc
     1501 gtcgcccgct gggggaggtc ttagtcgctt ggctgcgcca ccggcgcctg ctgctggtac
     1561 tggacaactg cgagcaccta ctcgccggca tgtccgaggt ggccacattg ctcggcgcct
     1621 gtccggggct gcgagtgctg gccaccagcc ggactgccct ccacctggcg gaggagcagg
     1681 tggcgccgct ccaccccctg cccgtgcccg acctcgcgcg cgcgtcctcc gcaggggccg
     1741 ttggccaatc gccggccgtg gcgctgttcg tgcagcgcgc atcagccgtg gatcccacct
     1801 ttcagctgac cgacgtgaac gcgccgacgg tggcggaagt ctgtgtgcgg ttggacgggc
     1861 tgccgttggc aatcgagctc gcggcggcgc gggcgcgcgt cttgtcggtt gagcagatcg
     1921 ctgcgcacct ggacgaccgc ttccatctgc tcaccgccgg tccgcgggcg gcgctgccac
     1981 gccagcagac cctgcgggcg acggtggact ggagctatgc actcctctcg gagccggagc
     2041 gcgcgctgct gcgccgactg tcggtgttcg cgggcggctg gaccctcgag gccgccgagt
     2101 ccgtcgcggc gggcgacggc gtccagccct cggctgtgct cgacctgctg gcccagttgg
     2161 tcgacgagtc gctggtgatc gccgaggcac ggcgcggcgc catgcgctac cggctgctcg
     2221 agaccatccg cgaggacgcc cgcgaccgac tgcaagcggt cggcgatgcc gagcgcacgc
     2281 gggaccgcca cctcgcctat tacctcgccc tggccgaaga tgccgagcta aagctccgcg
     2341 gagccgaata ccagttgtac ctgaatcggc tggaggagga gcacgccaac ctgcgagcgg
     2401 cgttggggtg ggctctggcg tcgccggacg ggggcgaggc gtcgctccgg ctgagcggcg
     2461 cgctggcctg gtactggtgg ctgcggagtc accacgacga gggctggcgg tggctgcaac
     2521 gcgccctggc cgggacagcg gacacctcgg cggcgcgcat gaaggcgctt tacggcgcgg
     2581 gtttcttggc gcatcaccgg cgtgactcca cgttggctcg cgcgctgctg gatgaaagcc
     2641 tggcgatcgc gcgcgcccgg gacgaccgct ggacagtcgc gtgggtgctc caccatctgg
     2701 gtcgggtcgc ctatttcgac gacgatcccg ctgcggcgcg tgcgctgggt gaggagagcc
     2761 tggcggtggc ggaagcgata ggcgaccgtt ggctaaacgc gtggccgctg cacctgctgg
     2821 gtctggcggc gcacatcgct ggcgactact cgagtgcgcg ggaatactac gcgcgcagct
     2881 tggcgatccg gcgggagttg ggctaccagg agggcattgg catcctgctc gtcctgctgg
     2941 gagtcgtcgc gctgcgcgaa ggcgacctcg cccgcgcgca cgcactgttt cgagagggtg
     3001 tggagggcgc ccagaccgtg ctggggccgt ggggcttggc catgccgctg gcgggttttg
     3061 cccatctcgc ggctgcgtct gggcagccgt tgcgggccac gcggcttggc gccgccgcgg
     3121 cagcgcagag cgaggcgtct cacacgccat tgatccccct cttcgatgcg ctgctccggg
     3181 aggggctgga ggccgcgcgg cgggccctcg atgagggcgc ctacgccgcc gcgtgggcgg
     3241 aaggccgagt gctgtcactg gaggagagca tcgcggaagc gctcgccgtc gaggccggac
     3301 caccgactct gccgccagcg cctgcggcgc accctcgccg agatggcgcc ttcgcgcagc
     3361 tcacgcccgc agagttcgag gtcctgcggc tgctgtccgg aggccggacc accagggaga
     3421 tcgccgccgg gctggtcgtc tccatctcca ccgtcgatcg tcacctcact cacctctatg
     3481 gcaagctcgg cgtccgcaac cgggccgagg caacggcgtt cgcgctgagg cacggcctgc
     3541 tctgaagacg gcggccgatt cgctgctccg tctccctcgt cgccacctct gagggaaatc
     3601 caccaggtac ctcagcgcgg tccagcacaa gatcccaggt ttcttggacg acggcgacgg
     3661 acatcgtggg tatgctgcga ctgtctccag atgccggatg acagccgaca cgctgcattg
     3721 agcgggccgc ccgtgcagtc ccgccaggaa aggcagaacg cgacccatga ccacctcaag
     3781 gcaaatcgcg accacctcgg tggacgatgc caccatcgaa gcgttccggg cagacttccg
     3841 tggagagctg agccggcccg gcgacacaac atacgacaag acccgtagcg tctttaacgg
     3901 gatgatcgac cgacatcccg cgctcatcgc gcggccgacc ggccccgccg acgtggtcgc
     3961 ggccgtgaag at
//
LOCUS       DASUBI010000346         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1145409, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000346 DASUBI010000000
VERSION     DASUBI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145409"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFU72_06690"
     CDS             complement(<1..722)
                     /locus_tag="VFU72_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEU5423207.1"
                     /translation="MTNTRELERDIDLQGRLAAFSISDLFQMLSFTRKTGTVTLIQGW
                     NSRTVTFEEGRISYVAAGSRLPSLTELLLRLGKVSREQVEGLYTNGIRGEDALLRHLL
                     EIGAVDEGDLRRCQEQLLEISIYTLFLWRNCVFTFHAGDVVREGGVAVDVDSMQIIIE
                     GTRRVDEWIEVSPVVPSVYMIFRARRRQPVGPPSFDLRLVFALVDGRRDVVAIARQAG
                     LTQFQTARALYHLVRAGYIEAVP"
     gene            complement(743..1738)
                     /locus_tag="VFU72_06695"
     CDS             complement(743..1738)
                     /locus_tag="VFU72_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU5423208.1"
                     /translation="MRALRPLGVLLGLLLLLPLPSLTGAAPQPVYIALGDSIAAGLVT
                     SLPRERGYPALVDELMPGTRLVNLAVPGETTERFMNRQLQRFQQEIGQLKASGAGLRA
                     VTITLGGNDVLALQGQSAAERQAGLDRFKSAYPLALAAIRDALGDLKPAVVVTTYYDL
                     SEGDPNQRGSDAWWLHQFNDVIRAAAQAQGMKVADLEPAFRGQIGQWTWYPNDIHPNN
                     AGHAAIARLVWTALGFDTQAPSIAVQSPGAGTLERRTPTVRVAASDNVGVTDVSLWVD
                     GKEIGGLLYVPAQQAYIGVWDARDYPRARATLTIKAHDLAGNEATADISVALPGS"
     gene            complement(1735..>2086)
                     /locus_tag="VFU72_06700"
     CDS             complement(1735..>2086)
                     /locus_tag="VFU72_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5423209.1"
                     /translation="IALRRSADGSLVLPKGHIEADEAAWQTAVREVREEMGLLAQVAG
                     WAGTLRITSRGIERHIAFLTATAEPAPEFADHLGHDTFLFTPEDAVQALSHEPARALL
                     RETLARAAWTGAGP"
BASE COUNT          307 a          745 c          721 g          313 t
ORIGIN      
        1 ggaaccgcct cgatgtagcc ggcccgtacc aggtggtaga gcgcgcgggc ggtctggaac
       61 tgggtcaggc ccgcctggcg ggcgatcgcc accacgtcgc ggcggccatc gaccagggcg
      121 aacaccaacc gtaggtcgaa ggagggcggg ccgacaggct ggcgccgacg ggcgcgaaag
      181 atcatgtaga ccgagggcac caccgggcta acctcgatcc attcgtcgac ccggcgcgtc
      241 ccctcgatga tgatctgcat gctgtcgacg tcgaccgcca cgccgccctc acgcacgacg
      301 tcgcccgcgt ggaaggtgaa gacgcaattg cgccagagaa agagggtgta gatggagatc
      361 tccagcaatt gctcctggca gcggcgcaga tctccctcat ccaccgcgcc gatctcgagc
      421 aggtggcgca gcagcgcgtc ctcgccgcgg atcccgttgg tgtagaggcc ttccacctgt
      481 tcgcggctga ccttccccag ccgcagcagc agctcggtca gcgacggcag gcggctgccg
      541 gccgcgacgt agctgatgcg gccctcctcg aaggtcacgg tgcgcgagtt ccagccctgg
      601 atcagcgtca ccgtcccggt cttacgggtg aagctgagca tctgaaagag gtcggagatg
      661 ctgaaggcgg ccaggcggcc ctgcaaatcg atatcccgct ccagctcgcg cgtgttggtc
      721 atcgccccgt tccctcccgc tcctagctgc ccggcagcgc gacgctgatg tcggccgtgg
      781 cctcgttgcc cgccagatcg tgtgccttga tcgtcagcgt ggctcgggcc cgcgggtaat
      841 cccgcgcgtc ccagacaccg atgtaggcct gttgcgccgg gacgtacagc aggcccccaa
      901 tctctttgcc gtcgacccag agcgagacat cggtcacgcc cacgttgtcg ctggccgcga
      961 cacgcaccgt cggcgtgcgc cgctccagcg tgccggcacc gggcgactgt acggcgatgc
     1021 tcggcgcctg ggtatcgaag ccgagcgcgg tccagaccag gcgcgcgatg gcggcgtgcc
     1081 cggcgttatt ggggtggata tcgttgggat accacgtcca ctggccgatc tggccgcgga
     1141 aggccggctc cagatcggcg accttcatcc cctgcgcctg ggctgccgcg cggatcacgt
     1201 cgttgaactg gtgcagccac caggcatccg agccccgctg gttcgggtcg ccctcgctca
     1261 ggtcgtagta ggtcgtcacc acgacggccg gcttgagatc gcccagggcg tcgcggatgg
     1321 cggccagcgc cagggggtag gccgatttga accggtcgag gcccgcctgc cgctcggcgg
     1381 cgctctggcc ttgcagcgcc agcacgtcgt tgccccccag cgtgatcgtc accgcccgca
     1441 ggccggcacc gctcgctttg agctgcccga tctcctgctg aaaacgctgg agctgccggt
     1501 tcatgaaacg ctcggtggtc tctcccggca ccgccaggtt gaccagtcgc gtgcccggca
     1561 tcagctcgtc gaccagcgcc gggtagccgc gctcgcgcgg caggctggtc accagcccgg
     1621 ccgcgatcga gtcgcccagc gcgatgtaga ccggctgggg cgccgcgccg gtcagcgacg
     1681 gtagggggag gagcaggagc aggccaagga gcaccccgag cggccgcaga gccctcacgg
     1741 cccggctcca gtccaggcag cgcgcgccag cgtctctcgc agcagcgcgc gcgccggctc
     1801 gtggctgagc gcctgtacgg cgtcctcggg cgtgaagagg aaggtgtcat ggcccaggtg
     1861 atcggcgaac tccggcgcgg gctcggcggt cgcggtcagg aaggcgatgt gccgctcgat
     1921 gccgcggctg gtgatgcgca gcgtgccggc ccagcccgcc acctgcgcca gcaggcccat
     1981 ctcctcgcgc acctcccgca cggccgtctg ccaggcggcc tcgtccgcct cgatgtgccc
     2041 cttgggcagc accagcgagc cgtccgccga gcggcgcagc gcgatc
//
LOCUS       DASUBI010000347         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_788215, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000347 DASUBI010000000
VERSION     DASUBI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788215"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="fusA"
                     /locus_tag="VFU72_06705"
     CDS             <1..299
                     /gene="fusA"
                     /locus_tag="VFU72_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870944.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5423210.1"
                     /translation="LEPVMRLKITVPENFVGDVMSDLNTKRGQVQNMAPGDNGTTIEA
                     LVPAAESQRYATDLRSITQGRGSFSAEFDHYQPVPAHLTDQIIAERKAKAEAHA"
     gene            436..837
                     /locus_tag="VFU72_06710"
     CDS             436..837
                     /locus_tag="VFU72_06710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEU5423211.1"
                     /translation="MADLSIVFDGGSKGNPGFGYGSFLLEDRAGFHEITRLEYGDAIT
                     NNQAEYRTLIAALEQALAHAGRRGWAPAALSLTVRSDSQLVIEQVLGRWKIRHPGLQP
                     LASEARRLIAHFGRADLTWQPRAQTVKVLGH"
     gene            910..>2430
                     /locus_tag="VFU72_06715"
     CDS             910..>2430
                     /locus_tag="VFU72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEU5423212.1"
                     /translation="MTPDPGQAIAHTKNALGRQQPLTEHLEAVSRLAGDFASQFGAEE
                     LATWLGRWHDIGKFNPAFQQYLRAAEADPTHRRHGPDHKAAGAQLAAEAAGPAALVVQ
                     GHHGGLTTPAGFVAWLKERAADPAVADAIALAREAYPALARRDELIEPPHAAGIEPPD
                     LELFIRMLLSAVVDADRLDTERHFAPERAASRGLVLEMDELWTRFARNQESFDRERRP
                     NGPREEEILRIRRAIYDDCLAAADEAPGLFRLTVPTGGGKTRSALAFALRHARRHGLR
                     RVVVAVPFITITEQTADIYRTILESPEDQEPVVLEHHSGVDAGDDEAGDAGSEEVWRR
                     LTAENWDASVVVTTTVQLFESLFANHTSPARKLHRLARSVIILDEAQALPPPLLTPAL
                     DVLRQLCERYGCTVVLSTATQPAFEAIGAFATLPAREIVPEPERYFEPLRRVDYTILR
                     AEPLDWPAAADLLRPEPQALAVLNTKAAAIALLDALDDREALHLSTLLCGGHRRQVL"
BASE COUNT          396 a          881 c          800 g          353 t
ORIGIN      
        1 tcctggagcc ggtgatgcgg ctcaagatca ccgtgccgga gaacttcgtc ggcgacgtga
       61 tgagtgacct gaacaccaag cgcggtcagg tgcagaatat ggcgcccggc gacaacggca
      121 ccacgatcga ggcgttggtg ccggcagccg agtcccagcg ctacgccacc gacctgcgct
      181 cgatcaccca ggggcgcggc tcgttcagtg ccgagttcga ccactaccag ccggtgccgg
      241 cgcacctgac cgaccagatc atcgccgaac gcaaggccaa agccgaagcg cacgcgtagg
      301 cgcgcaacgc gaaaactggc gcaccgtagc acaccgttgg ggcgggtttg aaacccgccc
      361 tctccttcca accccactcg gccacgttcg agcaggcatt ttgcgtgcgg cgcgcgcacg
      421 agaggattcg ggttcttggc agatctctct atcgtcttcg atggcggctc gaagggtaac
      481 cccggcttcg gctacggcag tttcctcctt gaggaccgcg ccggcttcca cgagatcacc
      541 cggctcgaat acggcgacgc catcaccaac aatcaggccg agtaccgtac cctgatcgcc
      601 gcgctcgagc aggcgctggc ccatgccggg cggcgcggct gggcgccggc ggcgctctcg
      661 ctcaccgtcc gctccgacag ccagctcgtc atcgagcagg tgctcggccg ttggaagatc
      721 cgccaccccg gcctgcagcc gctggccagc gaagcccgcc gcctgatcgc ccacttcggc
      781 cgcgccgacc tcacctggca gccccgcgcc cagaccgtca aagtgctcgg gcattgagca
      841 gcgcgcacgt gtgtccgcgg cgaggaggcg gagcgggcag gtgcggatgg catgaaaagg
      901 gaattgaaga tgacgccgga tccagggcag gcgatcgctc acacgaagaa cgcgctcgga
      961 cgacagcagc cgctcaccga gcacctggag gcagtatctc ggctggcggg agatttcgcc
     1021 tcacagtttg gtgcggagga actcgccacc tggcttgggc gctggcacga catcggcaag
     1081 ttcaaccccg cattccagca gtacctgcgc gcggccgagg ccgaccccac ccaccgtcgc
     1141 cacggcccgg atcacaaggc ggccggcgcg caactggcgg cagaggcagc cggtcccgca
     1201 gctctcgtcg tccaggggca ccacgggggc cttaccactc cggcaggctt cgtcgcgtgg
     1261 ctcaaggagc gcgccgccga tcccgccgtc gccgacgcga tcgcgctcgc acgcgaggcc
     1321 taccccgctc tggcccggcg cgacgaactc attgagccgc cgcacgccgc cggcatcgag
     1381 ccccccgacc tggagctgtt cattcgcatg ctgctctctg ccgtcgtcga cgctgaccgg
     1441 ctcgacaccg agcggcattt cgcgcccgag cgcgccgcct cgcgcggctt ggtgctggag
     1501 atggatgagc tgtggacgcg gttcgcgcgg aatcaggagt ccttcgaccg ggagcgcagg
     1561 cctaacggac cgcgagaaga ggagatcctc cgcatcagac gggcgatcta cgatgattgc
     1621 ctggcggcag ccgacgaggc tcccggactc tttcgcctga cggttccgac cggtggcggc
     1681 aagacacgct cggcgctcgc gttcgcgctg cgccacgcgc ggcggcacgg gctgcggcgc
     1741 gtggtggtgg cggttccttt catcaccatc acggagcaga ccgccgacat ctatcgcacc
     1801 atcctagagt ctcccgagga ccaggagccg gtcgtgctcg agcaccacag cggcgtggac
     1861 gccggcgacg acgaggcggg cgacgccggc tccgaagagg tctggcggcg actcaccgcg
     1921 gagaactggg acgcttcggt cgtggtgacg accacggtgc agctcttcga gagcctgttt
     1981 gccaaccaca ccagcccggc gcgcaagctc caccggctcg cccgcagtgt catcatcctc
     2041 gacgaggcgc aagcgctgcc cccgccgctg ctgacgccgg cgctggacgt gctgcgccag
     2101 ctctgcgagc gctacggctg caccgtcgtc ctctccaccg ccactcagcc agccttcgag
     2161 gcgatcggcg ccttcgcgac gttgcctgcc cgcgagatcg tgcctgagcc agagcggtac
     2221 ttcgagccgc tgcgccgggt agactacacg atcctgcgcg ccgagccgct cgattggccg
     2281 gccgccgccg acctgctgcg cccagagccg caggcgttgg ccgtgctcaa caccaaggcc
     2341 gccgccatcg cgctactgga cgcgctcgac gatcgcgagg cgctgcatct ctcgacgctg
     2401 ctctgtggcg ggcaccggcg gcaggtgctg
//
LOCUS       DASUBI010000348         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_734725, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000348 DASUBI010000000
VERSION     DASUBI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734725"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /gene="ileS"
                     /locus_tag="VFU72_06720"
     CDS             complement(<1..2508)
                     /gene="ileS"
                     /locus_tag="VFU72_06720"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5423213.1"
                     /translation="MFKPVSSKVDFPAKERETLSWWYSNGIVERYLKKNDQSERRWSF
                     LDGPITANNPMGVHHGWGRTYKDIYQRFHTMLGDKQRYQNGFDCQGLWVEVEVEKELG
                     FSSKRDIEAYGIDRFVEKCKERVWKFAGVQTEQSKRLGQFMDWDNSYYTMSDENNYTI
                     WHFLKVCHEHGWIYRGQDVMPWCPRCATGLSEMELSEGYQERTHRSLYVRFPLAGRPG
                     EYLLVWTTTPWTLAANVAAAVNPELTYAKVRQGDDTYYVAREAVPHAIHGEHEVVGEV
                     SGQDLLGWPYRGPFDELPAERGVQHQVIPWSDVSATEGSGIVHIAPGAGREDFRLGRE
                     HGLPVVAPIDENGVYVEGFGWLSGQRASAVSSEIEQNLREKGLLYHAYNHTHRYPTCW
                     RCGTDLLFRNVEEWFIAVDPWREEVMNVARQVQWIPDFGLERELDWLRNMDDWMISKK
                     RYWGLALPIWTCDDCGWFDVVGSETELEARAVAGWEEFAGHSPHKPWIDAVKIRCDAC
                     GGVASRIPDVGNPWLDAGIVAYSTLHYRDDRDYWREWFPADFITESFPGQFRNWFYSL
                     LAQSTVLTEQPPMKTVLGFATLRDEHGEEMHKSKGNAIWFEDAADNMGADVMRWLYLK
                     HNPAANLNFGFTLGDEARRAFILPLWNSYAFFVNYAALDGFNPLQHEVPLAERTLLDR
                     WIVSRLQELIGLVRERLFAYDNLTAARAIEDFVVEELSNWYIRRNRRRFWKAESDRDK
                     IAAYLTLHEVLVGVTTLLAPFTPFITEEMYQNLVRAVDTAAPESVHLTAYPEADEAKI
                     DRELTRDMAVLLHVVGLGRSARSQAGVKVRQPLAQVLV"
     gene            complement(3145..4494)
                     /locus_tag="VFU72_06725"
     CDS             complement(3145..4494)
                     /locus_tag="VFU72_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5423214.1"
                     /translation="METTDALGVLQQLRTQFHRTVLGRRKDTLFELADALLVADGPQP
                     LVHYSLSPAVQHGWPSLPDALSAGTLDAAALQRLLLPYLPVPERGHPVWVVDGTTWPR
                     PAAVTSPERTWCYRPSAGIPQHGLVPGWAYQWLVAVPEPGTSWVVPLDITRRGPEAAT
                     PTQVAVAQLRAVLPHVPPGPEPVLTADTGYDLGDLLTTCHPDAPDRVAVTPLLRLNRR
                     RCVAAPPAPYPGRGRRPTYGPKLHLRDPASLPPPDQSLILADPRHDTLTIDLWRDMRV
                     RPHGQYPFCLVRLQAARLPQSARPPDPLWLAWCAPTPPADLGDLWRWYSLRFTVEHGF
                     RFSKHELGWTTVRLRAPAAADRWSQLILVTWWQLWLARPLVADAHLPWERDLPPARLT
                     PRRVRRALRAILPTLGSPVPTLQPRGKSPGRRPGVQPDPHPHQPLVKRPARAPPKAA"
BASE COUNT          727 a         1565 c         1723 g          740 t
ORIGIN      
        1 gaccagcacc tgcgccagcg gctggcgcac cttgacgccg gcctgactgc gcgccgagcg
       61 gcccagcccg accacgtgca gcagcaccgc catgtcgcgg gtcaattcgc ggtcgatctt
      121 ggcctcgtcg gcctcgggat aggcggtcag gtgcaccgac tccggcgcgg cggtgtcgac
      181 cgcgcgcacc agattctgat acatctcctc ggtgatgaag ggggtgaagg gcgccagcag
      241 cgtggtgacc cccaccagca cctcgtgcag cgtcaggtag gcggcgatct tgtcgcggtc
      301 gctctcggcc ttccagaagc gccgccggtt gcgccggatg taccagttcg acagctcctc
      361 gaccacgaag tcctcgatgg cgcgcgcagc cgtcaggttg tcgtaggcga agaggcgctc
      421 gcgcaccagc ccgatcagct cctgcaggcg cgagacgatc cagcggtcga gcagggtgcg
      481 ctcggccagc ggcacctcgt gctgcagcgg gttgaagccg tcgagcgcgg cgtagttcac
      541 gaagaaggcg taggagttcc acagcggcag gatgaaggcg cgccgcgcct cgtcgcccag
      601 ggtgaagccg aagttgaggt tggccgccgg gttgtgcttg agatagagcc agcgcatcac
      661 gtcggcgccc atgttgtcgg cggcgtcctc gaaccagatg gcgttgccct tgctcttgtg
      721 catctcctcg ccgtgctcgt cgcgcagcgt ggcgaagccg agcaccgtct tcatcggcgg
      781 ctgctcggtc agtaccgtgc tctgagccag cagcgagtag aaccagttgc ggaactggcc
      841 ggggaaggac tcggtgatga agtcggccgg gaaccactcg cgccagtagt cgcggtcgtc
      901 gcggtagtgc agcgtcgagt aggcgacgat gccggcgtcg agccacgggt tgccgacgtc
      961 ggggatgcgc gaggcgaccc cgccgcaggc atcgcagcgg atcttgaccg cgtcgatcca
     1021 gggcttgtgc ggcgagtgcc cggcgaactc ctcccagccc gccaccgcgc gcgcctccag
     1081 ctcggtctcg ctgccgacca cgtcgaacca gccgcagtcg tcgcaggtcc agatcggcag
     1141 cgccaggccc cagtagcgct tcttggagat catccagtcg tccatgttgc gcagccagtc
     1201 cagctcgcgc tccagaccga agtcggggat ccactgcacc tggcgggcga cgttcatcac
     1261 ctcttcgcgc cagggatcga cggcgatgaa ccactcctcg acgttgcgga agaggagatc
     1321 ggtgccgcag cgccagcagg tcggatagcg gtgggtgtgg ttgtaggcgt ggtagagcag
     1381 ccccttctcg cgcaggttct gctcgatctc gcttgacacc gcgctggccc gctggccgct
     1441 cagccagccg aagccctcga cgtagacgcc gttctcgtcg atcggcgcga ccaccggcag
     1501 gccatgctcg cggcccaggc ggaagtcctc gcggccggcg cccggcgcga tgtgcacgat
     1561 gccgctgccc tcggtggcgc tgacgtcgct ccaggggatc acctggtgct gcacgccgcg
     1621 ctcggccggc agctcgtcga aggggccgcg gtagggccag ccgagcaggt cctgcccact
     1681 gacctcgccg acgacctcgt gctcgccgtg gatggcgtgc ggcacggcct ctctggccac
     1741 gtagtaggtg tcgtccccct ggcgcacctt ggcataggtc agctcggggt tgaccgcggc
     1801 cgccacgttg gcggccagcg tccagggggt ggtggtccag accagcaggt actcaccggg
     1861 ccggcctgcg agcgggaagc gcacgtagag gctgcgatgg gtacgctcct ggtagccctc
     1921 gctcagttcc atctcggaga ggccggtggc gcagcgtggg caccagggca tcacgtcctg
     1981 gccacgatag atccagccgt gctcgtggca gaccttcagg aagtgccaga tggtgtagtt
     2041 gttctcgtcg gacatggtgt agtaggagtt gtcccagtcc atgaactggc ccaggcgctt
     2101 ggactgctcg gtctgcactc cggcgaactt ccagacgcgc tccttgcact tctcgacgaa
     2161 gcggtcgatg ccgtaggcct cgatatcgcg cttggaggag aagcccagct ccttctcgac
     2221 ctcgacctcg acccagagcc cctggcagtc gaagccgttc tggtagcgct gcttgtcgcc
     2281 gagcatggta tggaagcgct ggtagatgtc cttataggta cggccccagc cgtggtgcac
     2341 gcccatcggg ttgttggccg tgatcgggcc gtcgaggaac gaccagcgcc gctccgactg
     2401 atcatttttc ttgagatagc gctcaacgat cccgttgctg taccaccacg agagcgtctc
     2461 gcgctctttg gccggaaaat cgaccttgct cgataccggt ttgaacatca gcatgcctcc
     2521 gtatgggccc tcaccccccg cgggggttgc cctcaccccg gccctctccc gtgcgcggga
     2581 gagggggcgc gacctttcgt gccggctccc cttctcccgc gcacgggctc cgatcgagaa
     2641 ggggttgggg gatgagggcc accccaacac gcacacaaaa accccgtcct cggttgagga
     2701 cggggggaac ccgcggtacc acctcagttg gccccaagtg aggcccactc agccggatgc
     2761 cgcccacgag tgggtgcgca tccgtgccgc gctaacgggc ggcgacccgg ccacgtctac
     2821 taggcttgcg ccgttcgggt ggcggctcgg gaaggatctt ccgcggggtt catagcatcc
     2881 ggctcgcacc agccccggac tcgctgcgcc ggcggccccg cgtactcgtt tccgtcacag
     2941 ccgctcaacg atattgaccc cgaaggatac cacagaattg cgtttccagc aacttcgttg
     3001 gggccgtgcc catcgaggag cattggtggc gctccgggac ggatccttcg actgcgctca
     3061 ggatttggcg tttggacaaa gcagataccg tccgggatac agtgcaaccg gactggcggg
     3121 acgggtggcg gcgtgggcgg ggcctcaggc ggctttcggc ggggcgcggg cgggccgttt
     3181 gaccagcggc tggtgcgggt gggggtcggg ctggacgccc ggacggcgtc cgggagactt
     3241 tcctcggggt tggagcgtgg ggaccggact gcccagcgtc gggagaatcg cgcgcagggc
     3301 gcggcggacg cggcgcgggg tgaggcgcgc gggcggcaga tcccgttccc aggggaggtg
     3361 cgcatcggcg accaggggcc gcgccagcca gagttgccac caggtgacga ggatgagctg
     3421 actccagcga tcggcggccg ccggcgcacg cagccgcacc gtggtccagc cgagttcgtg
     3481 tttgctgaac cggaagccat gctcgacggt gaagcgcagg ctgtaccagc gccagaggtc
     3541 gcccaggtcg gcaggcgggg tgggggcgca ccaggccagc cagagggggt cgggggggcg
     3601 cgcagactgg ggcaggcgcg ccgcctggag gcgcaccaag cagaaggggt actggccgtg
     3661 cgggcgcacc cgcatgtcgc gccagaggtc gatggtcagc gtgtcatggc gcggatcggc
     3721 caggatcagc gactggtccg gtggcggcag gctggccggg tcgcggaggt ggagcttcgg
     3781 gccataggtg gggcggcgcc cgcggcccgg gtagggcgcc gggggcgcgg cgacgcagcg
     3841 acggcggttg aggcggagga ggggcgtgac cgccacgcgg tcgggcgcgt ccggatggca
     3901 ggtggtcagg aggtcgccca gatcatagcc ggtatcggcg gtcagcaccg gctcggggcc
     3961 gggtgggacg tgcggcagca cggcgcggag ttgggccacg gcgacctggg tcggggtggc
     4021 ggcctccggc ccgcggcggg tgatgtccag gggcacgacc caactcgtgc ccggctccgg
     4081 cacggccacc agccactgat aggcccagcc gggcaccagc ccgtgctggg ggatgccggc
     4141 gctgggccga taacaccagg tgcgctcggg gctggtgacg gcggctggac gcggccaggt
     4201 ggtgccatca accacccaga ccgggtggcc ccgctccggc accggcaggt acggcagcag
     4261 caggcgctgc agggccgcgg catcgagggt gcccgcgctc agggcatcgg gcagactggg
     4321 ccagccgtgc tgcaccgccg gactgaggct gtagtggacc agcggctggg ggccgtcggc
     4381 cacgagcagg gcatcggcca gttcaaacag ggtatccttg cgccgaccca gcacggtgcg
     4441 gtggaactgg gtgcgcagct gctgcaagac gccaagcgcg tcggtcgttt ccataccgct
     4501 ttcgcgccgc acgccgcaga ccacgcccct tccccgctcg gctgggtctc acattgtcta
     4561 aacacgagtc aggatgaccg ggagggtaag gggctgacga ggaggatggg tcttggacga
     4621 tggccctgct cgtccgaaag gccgcacccc ttcccccgtc atcctgagcg cagtcgaagg
     4681 atacgtcccc gacggctccc attcatgtcg aactccacac aacgcgaaac cgccgctccc
     4741 caagcggaga gcggc
//
LOCUS       DASUBI010000349         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1308920, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000349 DASUBI010000000
VERSION     DASUBI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308920"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1171)
                     /locus_tag="VFU72_06730"
     CDS             complement(173..1171)
                     /locus_tag="VFU72_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423215.1"
                     /translation="MTERAIQDTYELERLAAEAEVEHVIDESADLLDQATNDAVYRYF
                     RDVGGHRLLTHAEEIEYSRAVRAGLEVRKQLEAGEVETAEMRLILERAKQSREKLIRH
                     NLRLVVSIAKKYRASGLPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIRQAIG
                     RAVANLSRTIRVPVHMHDLISKVRRTEAQLEQQYGRPATDQELAQVLEMEVERIEQAR
                     SAIPRTSSLDKPIGEDGESTIGDLLPDPQSDEVVDQAVTGAIRDQIRRSLENLTERER
                     GVLELRFGLGEGNRQPQTLAEIAEHYGISRERVRQIEKEALAKLRKSDLIQLANAA"
     gene            1695..>2214
                     /locus_tag="VFU72_06735"
     CDS             1695..>2214
                     /locus_tag="VFU72_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423216.1"
                     /translation="MISGIDHLVILVPNLEQAITQYTDLGFQVVRGGEHPGGTHNALV
                     GFADGSYLELIAFQQPEQPNPHRWYRFLSAGCGLIDFALRCDDVDQAAARLREAGLDY
                     TVAPGARRTPRGVELQWRSAQPPQERAGELPFLIQDVTPRAERVPGGAQARQPNGVVG
                     VRGLVVAVRDLIE"
BASE COUNT          308 a          798 c          730 g          378 t
ORIGIN      
        1 gccacgtttc cccgttgccg aggcgtgggg gaagcagggc ggacacggcg gtccgcccct
       61 acaggacgcg cgtgatcgga ctgtacgtac aggagtgttc ctgctggcgg ctgcggcggg
      121 caagaaaaat ccccggtggg cgtgcccacc ggggagagct gcttccgcga aactaggcgg
      181 cgttggccag ttggatcagg tccgacttgc gcagcttggc tagggcttcc ttctcgatct
      241 gccgcacgcg ctcccggctg atgccgtagt gctcggcgat ctcggccagc gtctgcggct
      301 ggcggttgcc ctcacccagg ccgaagcgca gctcgagcac cccgcgctcg cgctcggtca
      361 ggttctccag gctgcggcgg atctggtcgc gaatggcgcc ggtcaccgcc tgatcgacca
      421 cctcgtcgct ctgcgggtcg ggcagcaggt cgccgatcgt gctctcgcca tcctcgccga
      481 tcggcttgtc gagcgacgag gtgcgcggga tcgccgagcg tgcctgctcg atgcgctcga
      541 cctccatctc gagcacctgg gccagctcct ggtcggtggc cgggcggccg tactgctgct
      601 cgagctgcgc ctcggtgcgg cggaccttcg agatcaggtc gtgcatgtgc accggcacgc
      661 ggatcgtgcg gctcaggttg gccaccgcgc ggccgatcgc ctggcgaatc cagaaggtgg
      721 cgtaggtgct gaagcggtag ccgagctcgg gatcgtagcg ctcgaccgcg gtcatcagcc
      781 caatgttgcc ctcctggatc aggtcgatca gcggcagccc actggcgcga tacttcttgg
      841 cgatcgagac gaccaggcgc aggttgtgcc ggatcagctt ctcgcgcgac tgcttggcgc
      901 gctccaggat caggcgcatc tccgcggtct cgacctcgcc cgcctccaac tgcttgcgga
      961 cttccagccc ggcacgcacg gcgcgcgagt actcgatctc ttcggcatgc gtcaggagtc
     1021 gatgccctcc gacatcgcgg aagtagcggt agacggcgtc attcgtcgcc tgatcgagca
     1081 ggtcggcgga ctcgtcgata acatgctcga cctccgcctc cgcggccagc cgctccagct
     1141 cgtaggtatc ttggatcgct cgctccgtca tgtgactccc agactccgac ttcttccagt
     1201 cgttcggttc cggcgcggcc cccactcagg ctgcatggtc cgaaaccttc gtagtatacc
     1261 gcattgcgta tacctttgtc aagaacgacc ggacgctcgc gcggttgcgc aatctttcac
     1321 aatcacagcg cgccccgcac gccccgccgg cgccgccgcc cctcccgggg gcatgactgc
     1381 gtgcacggac gaccggctgc gcctctacgt actctttata cgaacgacct ggccgctggt
     1441 tgcgcccctg tcacagcttc cacgccgccg cttgcggctg ccgcgaatcc cgcgccgcgg
     1501 gcaccggcga aggcagccat tcaccggaat gtacgtcgtg gagggccaag tcgatctata
     1561 ctcctctctg gccgccggcc cgcggagagt cgccggggcc agatcggacc cgcgacgtcc
     1621 gcgccagcga gtcaccgcac cccgtcggcg gcgcccgggc cggtcccgtg ctagctggaa
     1681 aggaagcagg ccgcatgatc tccggtatcg atcacctcgt catcctggtg cccaacctcg
     1741 agcaggcgat cacccagtac accgatctcg gcttccaggt ggtgcgcggc ggcgaacacc
     1801 cgggcggcac ccacaatgcg ctggtcggct ttgccgacgg ctcgtacctg gagctgatcg
     1861 ccttccagca gccggagcag ccgaacccac accgctggta ccgctttctc tccgccggct
     1921 gcggtctgat cgatttcgcc ctgcgctgcg acgacgtcga ccaggcggcc gcccgcctgc
     1981 gcgaggccgg cctcgactac accgttgctc cgggcgcgcg ccgcaccccg cgcggggtcg
     2041 agctgcagtg gcgctcggcc cagccgcccc aggagcgcgc cggcgagctg cccttcttga
     2101 tccaggacgt cacccctcgc gccgagcgcg tgccgggcgg cgcccaggcg cgccagccga
     2161 acggcgtggt cggcgtgcgc gggctggtgg tggcggtgcg cgacctgatc gagg
//
LOCUS       DASUBI010000350         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_890782, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000350 DASUBI010000000
VERSION     DASUBI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890782"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="alr"
                     /locus_tag="VFU72_06740"
     CDS             complement(<1..892)
                     /gene="alr"
                     /locus_tag="VFU72_06740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5423217.1"
                     /translation="MGEFAGRLSAARAGSHAVIDLDAYAGNLARMRARPQLPAEAALM
                     AVVKANAYGHGLLPIAQTAVEAGADWLGVARIEEGLLLRQAGIETPVLVLGPPNPALL
                     AQAVAAGLDLAAGSLETVAAIAAAATARQPARVHLKLDSGMHRYGIEPEGAVALARTV
                     AGERRLALAGLFTHFATADEPGGFMAEQIRRFEAARAALAAAGIAPPLLHYANSAGTL
                     RGAMPAPGAGQTAIVRCGYALYGLSPSAEVPVPLDFRPVLSLHSRLARVFTLPAGEGV
                     SYGLSFVAERPLRCATLPIGY"
     gene            complement(1016..1204)
                     /locus_tag="VFU72_06745"
     CDS             complement(1016..1204)
                     /locus_tag="VFU72_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423218.1"
                     /translation="MDESLKNKAKETASDLIEDASTEITEQAEELKDKAGKLFSKLQD
                     KLEDLTGGDDDKAKEKPA"
     gene            complement(1562..1774)
                     /locus_tag="VFU72_06750"
     CDS             complement(1562..1774)
                     /locus_tag="VFU72_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5423219.1"
                     /translation="MDESTKNKAKGTWEEAKGKAKEAAGDVTDDTSMQAEGQADELKG
                     KARKQFGEFQGKLDDLTDDEKRDKPS"
     gene            complement(1886..2374)
                     /locus_tag="VFU72_06755"
     CDS             complement(1886..2374)
                     /locus_tag="VFU72_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5423220.1"
                     /translation="MSEQDALLSAVRQGVSQVLREQHEAIRQTLAGLDTAALNWSPGP
                     EMNSIAVIFTHMLGAEDAITATVLGEPIERDRDAEFRVHDVEPDRLAARAEQVGQRAL
                     DRIERLTAAQLEAFHTPPRDRLGRRFLGSWWIFHVLEHNREHIGQAGLTRQLYQEQAN
                     HS"
     gene            complement(2399..>2646)
                     /locus_tag="VFU72_06760"
     CDS             complement(2399..>2646)
                     /locus_tag="VFU72_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HEU5423221.1"
                     /translation="QSGYLADTAGIRALGLVGVEPELLDHYFPEFRPELGRCYYADCT
                     HLDEPGCAVREAVEAGAIDRGRYESYRALRNPAEAED"
BASE COUNT          346 a          939 c          939 g          422 t
ORIGIN      
        1 cgtagccgat ggggagcgtg gcgcagcgta gcggccgctc ggccacgaag ctcaggccgt
       61 aggagacccc ctcgccggcc ggcagggtga agacgcgcgc cagccgggag tgcaggctga
      121 gcaccggccg gaaatcgagc gggaccggca cctcggccga gggcgagagg ccgtagagcg
      181 cgtagccgca gcgcacgatg gccgtctggc ccgcaccggg ggccggcatc gcgccgcgca
      241 aagttccggc gctgttggcg tagtggagca ggggcggcgc gatgccggcg gcggccagag
      301 ccgcgcgcgc ggcctcgaag cggcggatct gctcggccat gaagccgccc ggctcgtcgg
      361 cggtggcgaa gtgggtgaag aggccggcca gcgcgaggcg acgctcccct gcgacggtcc
      421 gggccagcgc gaccgcccct tccggctcga tgccgtagcg gtgcatgccg ctgtcgagct
      481 tgaggtgcac ccgcgccggc tggcgggccg tggccgcggc ggcgatggcg gcgacggtct
      541 ccaggctgcc ggcggccagg tcgagcccgg cggcgacggc ctgggccagc agcgcggggt
      601 tgggcgggcc gagcaccagc accggcgttt caatgcccgc ctggcgcagc agcagcccct
      661 cctcgatgcg cgccacgccg agccaatccg ccccggcctc aacggccgtt tgcgcgatgg
      721 gcagcaggcc gtggccgtag gcgttggcct tgacgacggc catcagcgcg gcctcagccg
      781 gcagctgcgg gcgcgcccgc atccgcgcca gattgccggc gtaggcgtcc aggtcgatga
      841 cggcgtggct gccggcccgc gccgccgaca gccgcccggc gaactcaccc atcaagcagc
      901 gctccccgcg tcagcccgcg cggcgtgcgg cccaagcgca cccagtaggc gacgggcaca
      961 accagcgtgc cggatgttcc cggcgccccg gcgccactgg gtggtggtac gaggactaag
     1021 ctggcttctc ttttgccttg tcgtcgtcgc cgccggtcag gtcctcgagc ttgtcctgga
     1081 gcttgctgaa gagcttgccg gctttgtcct tcagctcctc ggcctgctcc gtgatctcgg
     1141 tcgaggcgtc ctcgatgagg tcgctcgcgg tctctttggc tttgttcttg aggctctcgt
     1201 ccatacatcc ccctatagat atggccacgc atcataccga tgcgcggcgg catcgaatcg
     1261 cgcgcgatat ccctgggaga cactgccgtg cgtctagtat aagccatggc gggccggaat
     1321 acgcacgccg cggctgggag agccggcggg gcagaccagc ccggccgatg tatgcgcggt
     1381 tgtgcgccag gaggtggacg atccagcaac tgccggggga gcggcgcccc aggcggtcgc
     1441 gcggcggcgt gcggtacccc ttcatcgctc acctgtagcc cagcgggccg ccgccgggca
     1501 cgacgaacgc gccgggtgtt ccccggcgcg ctgccgttgc tcgtgagcga gcccgcgacg
     1561 actaggacgg cttgtcgcgc ttctcgtcgt cggtcaggtc gtcgagcttg ccctgaaatt
     1621 caccgaactg cttgcgggcc ttgcccttca gctcgtcggc ctggccctcg gcctgcatcg
     1681 acgtgtcgtc ggtgacgtcg cctgcggcct ccttggcctt gcccttggcc tcttcccagg
     1741 tgcctttggc tttgttcttc gtgctctcgt ccatgcttcc tcccataacc ctagacgcgc
     1801 accacaccga tgcgtgtcgc tttccaatct cgggcgatgc cctctgggcg gtaagaagca
     1861 atggtcatgc cataagatac ggtgctcagg aatgattcgc ctgctcctgg tagagctggc
     1921 gggtcagacc ggcctggccg atgtgctcgc ggttgtgctc cagcacgtgg aagatccacc
     1981 agctgccgag gaagcggcgc cccaggcggt cgcgcggcgg tgtgtggaac gcctccagtt
     2041 gcgcggccgt caggcgctcg atccggtcga gggcgcgctg ccccacctgc tcggcgcgcg
     2101 ccgcgagccg gtccggctcg acatcgtgca cgcggaactc ggcatcgcgg tcgcgctcga
     2161 tcggctcgcc cagtaccgtg gcggtgatgg cgtcctcggc cccgagcatg tgggtgaaga
     2221 tcaccgcgat cgagttcatc tcgggtccgg gcgaccagtt gagcgcggcc gtatccagcc
     2281 cggccagcgt ctgccggatg gcctcgtgct gctcgcgcag cacctgcgac actccctggc
     2341 gcaccgcgct cagcagcgca tcctgctcac tcatgcgtcc tcctgccggc gcgctgccct
     2401 agtcctcggc ctccgccggg ttgcgcagcg cgcggtagct ctcgtagcgg ccacggtcga
     2461 tcgccccggc ctcgaccgcc tcgcgcacgg cacagccggg ctcgtcgagg tgggtgcagt
     2521 cggcatagta gcaccggccc agctccgggc ggaactcggg gaaatagtgg tcgagcagct
     2581 ccggctcgac tcccaccaag ccgagcgcgc ggataccggc ggtgtcggcc aggtagccgc
     2641 tctggc
//
LOCUS       DASUBI010000351         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_631409, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000351 DASUBI010000000
VERSION     DASUBI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631409"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFU72_06765"
     CDS             <1..976
                     /locus_tag="VFU72_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEU5423222.1"
                     /translation="DAWRARAALGAGELARRILDFPRSAALLREALEIQRELGDADGI
                     AWSLVYLAYTLGMAGDFEGARAPAEESLALFRASGDQLGVARALNTLAEDARLRGDYG
                     VAAAYYREALTIDRQLGDPHGSAIRLHNLGYVALHEGDVRQAVRAFRESHQLHQALGY
                     RSGPLSFLEGMAASASAAGGAEQAARLYGAWEANCRLPGTEFTLHPPDQREYDRYVAR
                     ARDALGGQALAHAWESGRHLTLEQAIAEALAFAETLDQPARLTARERQVAALAAHGLT
                     NHQIALKLDIAERTVDAHVAHILRKLALSSRRQLAARLTGSDPEPFAC"
     gene            1118..1411
                     /locus_tag="VFU72_06770"
     CDS             1118..1411
                     /locus_tag="VFU72_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423223.1"
                     /translation="MVRHHCSFLVRWWLLDHGGSRIELEHIQSGARTIAGSPAEALAW
                     MQATAASESGPSGSEDGASAARGEEQSRRHTSGNAQTHDRSMGWSEEGKEGTL"
     gene            1408..1839
                     /locus_tag="VFU72_06775"
     CDS             1408..1839
                     /locus_tag="VFU72_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423224.1"
                     /translation="MKLRRIIATSLTTLALAGLVAVGGAGAAPLNSPREQTIALTCQD
                     NDLGISNVDLATLDNNGSWTPGHILTINGQPAAAIAVPLEFHFTFTFGDQSFSADQVK
                     PGSRNGITATATCSGSETITDEDGNTGTFDVTVTFFVAPRG"
     gene            1925..2650
                     /locus_tag="VFU72_06780"
     CDS             1925..2650
                     /locus_tag="VFU72_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423225.1"
                     /translation="MNARNIGSTGDDAAQRWGLWALAAGGALIAILTAFWMTGLGGGG
                     NDPAQQARDQASLLSVIGFGWLQILAFVALIFGLLALYAGARPAAPRAALAGLVLGIL
                     SLSAVIAAFGAAALGGAVVAHSYLNGNTGALDAMNHLSGGSFGAAIMQALVAGTILSV
                     LCAIAGGIALWREAAAPRWAIVAFGLGFVLAVASIPGISTVGGLLLIASGVVFTRSLL
                     PRATVVSESQGGAQARPQPTHGD"
     gene            2945..>3628
                     /locus_tag="VFU72_06785"
     CDS             2945..>3628
                     /locus_tag="VFU72_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5423226.1"
                     /translation="MATREELRQRIVDAIEAQRDEIVAVAEEIRQNPELGYQEVKASQ
                     LLASAIRDQGFEVEKPAAGIETAFVASKHGYKDGPHVAVLAEYDALAGIGHGCGHNLI
                     GASGLAAAIGLNAVMDELPGVFEVIGTPAEEGGAGKVKMERAGVFDDVDAALMIHHAG
                     NHSGAATEWPGGTCNALTQFTLEYFGKPAHAAADPYNGVNALNAVIEFFKGVDALRQH
                     VPMETRIHGI"
BASE COUNT          580 a         1272 c         1265 g          511 t
ORIGIN      
        1 agacgcgtgg cgggcgcgcg cggcactcgg cgccggggag cttgcgcggc gtatcctcga
       61 ctttcctcgc tccgccgcgt tgctacggga ggcactggag atccagcgcg aactgggcga
      121 tgcggatggc atcgcctggt cgctggtcta tctcgcctac acgctcggca tggccggcga
      181 cttcgagggg gcgcgcgcgc ccgccgagga gagcctggcg ctcttccgcg ccagcgggga
      241 tcagctcggc gtggcccggg cgctcaacac gctggccgag gacgcccggc tgcgtgggga
      301 ttacggcgtg gcggccgcgt actaccggga agcgctgacg atcgaccgcc agctcggcga
      361 cccgcacggg agcgcgatcc gcctgcacaa cctcggctat gtggcgctgc acgagggaga
      421 cgtacgccag gccgtgcgcg cctttcgcga gagccaccaa ctgcatcagg cgctcggcta
      481 ccgctccggc ccgctctcgt ttctggaggg catggccgcg tccgccagcg ccgccggagg
      541 cgccgaacag gcggcacgcc tctacggcgc ctgggaagcc aactgccggc tgcctggcac
      601 ggaattcacg cttcacccac ccgaccagcg cgagtacgac cggtacgtcg cgcgagcccg
      661 cgacgcgctg ggcggacagg cgctcgccca tgcctgggaa tccggccggc acctgacgct
      721 ggagcaggcc atcgccgaag cgctcgcgtt cgcggagacg ctggaccagc ccgcgcgcct
      781 gaccgcgcgc gagcgccagg tcgccgcgct ggccgcgcac ggcctgacca accaccagat
      841 cgccctgaag ctcgacatcg ccgagcgcac cgtcgacgcg cacgtcgcgc atatcctgcg
      901 caagctcgcg ctcagctcgc gccggcagct cgccgcccgg ctgaccgggt cggaccccga
      961 acccttcgcc tgctgaatcc agctctcgcg cccggttcat acccgcatag gtagacctag
     1021 ttccccgatc tcgcccatct cgtaacagcc ggcaaacatc agactggtag gctggcctca
     1081 tcacccgctg tcgaggcagg ggaaggttcg cggcgcgatg gtacggcacc actgctcgtt
     1141 cctggttcgc tggtggctgc tggaccatgg cggctcccgc atcgaactgg agcacatcca
     1201 gtcgggcgcc agaaccatcg ccggctcgcc cgccgaggcg ctcgcctgga tgcaggcgac
     1261 ggccgcgagc gagtccggcc cgagcgggtc cgaagatgga gcgagcgccg cgaggggaga
     1321 ggagcagagc cgccggcaca caagcgggaa cgcgcagacc catgaccgct cgatgggttg
     1381 gagtgaggaa ggcaaggagg ggacactgtg aaactacgac gcatcatcgc gaccagcctg
     1441 acgaccctgg cgctggccgg gctcgtcgcg gttggcggcg ccggcgcggc gccgctcaac
     1501 tcaccgcggg agcagacgat cgcgctgacc tgccaggaca acgacctcgg catcagcaac
     1561 gtcgatctcg cgacgttgga caacaacggc tcctggacgc cgggccacat cttgacgatc
     1621 aacggccagc cggccgccgc gatcgccgtt ccgctggagt tccacttcac cttcaccttt
     1681 ggggaccagt ctttctcggc cgaccaggtc aagccgggca gtcgcaacgg catcacggca
     1741 acggcaactt gcagcggcag cgagaccatc acggacgaag acgggaacac gggaaccttc
     1801 gacgtcacgg tgacgttctt cgtcgcgccg cgcggctagg caccatgagc ggcccgagcg
     1861 agcggcccaa gctgggcatg ctcgctcgac cgcggaacaa ccagtggaga gaagggtgag
     1921 acacatgaat gcacggaata ttggatcaac gggtgacgac gccgcccagc gctggggcct
     1981 atgggcgctg gcggccggcg gggcgctgat cgcgatcctg accgccttct ggatgaccgg
     2041 cctcggcggc gggggaaacg acccggcgca gcaggccagg gatcaggcga gtctcctcag
     2101 cgtcatcggc ttcggctggc tgcagatcct ggccttcgtg gcgctgatct tcgggctgct
     2161 ggcgctctat gccggtgcgc gaccggccgc tccgcgggcg gcgctcgccg ggctcgtgct
     2221 cggcatcctg agcctcagcg ccgtgatcgc cgcttttggc gccgcggcgc tcggcggtgc
     2281 ggtcgtggct cattcgtatc tcaatgggaa taccggggcg ctcgacgcca tgaatcacct
     2341 gtcgggcggc agctttggcg cggctatcat gcaggcgctg gtggcaggca cgatcctcag
     2401 cgtcctctgc gccatcgcgg gcggtatcgc cctctggcgt gaggcggccg cgccgcgctg
     2461 ggcgatcgtc gccttcggcc tcggctttgt tctcgccgtc gccagcattc ccgggatctc
     2521 gaccgtcggc ggcctgctgc tgatcgccag cggcgtcgtc ttcacccgct cgctgttgcc
     2581 gcgcgcgacc gtcgtctcgg agagccaggg cggggcccag gcccggccgc agccaacgca
     2641 cggagactaa cggcacgctg acagcagccc acggctcgat cgtcgtccag tagcaacagc
     2701 gaccgaacac ctgactgccg ggcaagggcg gcggagcgtc cccaagacgc gcaccgccgc
     2761 ccggcgcccg tcgtcctatg gcggctcgtt tccacccatc tcgtcgccac tccataccgg
     2821 acgcgccagg tcagcctgcc ctccatccgg acgccggacc gctagccacg atgcccggcc
     2881 ggtggtatgg tgcgcccaaa ctggcaggcg aatagtgagc cttaatgcag agatggaggg
     2941 accagtggcg acacgggagg agctgcggca gcgcatcgtg gacgcgatcg aggcccagcg
     3001 ggacgagatc gtcgcggtgg ccgaggagat ccgccagaac cccgagctcg gctaccagga
     3061 ggtcaaagcc tcgcagctct tggcctcggc catccgggac cagggcttcg aggtcgagaa
     3121 gccggccgcc ggcatcgaga cggccttcgt ggccagcaag cacggctaca aggacggacc
     3181 ccacgtggcg gtcttggccg agtacgacgc gttggcgggc atcggacacg gctgcgggca
     3241 caacctgatc ggggcctcgg ggctggcggc ggccattggg ctgaacgccg tcatggatga
     3301 gctgccgggg gtcttcgagg tgatcggcac cccggccgag gagggcggcg cgggcaaggt
     3361 caagatggag cgcgccggtg tcttcgacga cgtcgacgcg gcgctgatga tccaccacgc
     3421 cggcaaccac tccggtgcgg ccaccgagtg gcccggcggc acctgcaacg ccctgaccca
     3481 gttcacgctg gagtacttcg gcaagccggc gcacgcggcg gccgacccct acaacggggt
     3541 caacgcgctc aatgcggtga tcgagttctt caagggggtg gacgcgctgc ggcagcacgt
     3601 gccgatggag acgcgcatcc acggcatc
//
LOCUS       DASUBI010000352         5704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_944734, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000352 DASUBI010000000
VERSION     DASUBI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944734"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1017)
                     /gene="pdxS"
                     /locus_tag="VFU72_06790"
     CDS             complement(136..1017)
                     /gene="pdxS"
                     /locus_tag="VFU72_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5423227.1"
                     /translation="MEKSTWTTKAGLAQMLKGGVIMDVVTAEQARIAEEAGACAVMAL
                     ERVPADIRAQGGVARMSDPELIIQIKEAVTIPVMAKARIGHFAEAQILEEIGVDYIDE
                     SEVLTPADERFHINKHEFKVPFVCGARDLGEALRRIGEGAAMIRSKGEAGTGNIVEAV
                     RHLREITDGIRRLQSLPKEELMSVAKELGAPYDLVVEVAETGKLPVVMFCAGGIATPA
                     DASLTMQLGAEGNFVGSGIFKSDNPYERGKAIVEATTHFRNPKVLAEVSRGLGEAMRG
                     LELASIPAEQQLAKRGW"
     gene            complement(1076..2611)
                     /locus_tag="VFU72_06795"
     CDS             complement(1076..2611)
                     /locus_tag="VFU72_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5423228.1"
                     /translation="MLVELDRADETPLYIQISRQLREQIRGGSLTPGYRLPPERRLAA
                     MLGVNRTTVVNAYRELAGDGLIISRVGQGTRVAQPGELLAVPDEPALALGRSPARLPW
                     EQLFTAATEVAQDPLLADVQLVAARPDVISFASGVPSPDLYPIEQIRSLLDEALHQSG
                     QSLLQYCPTEGYMPLREVLADWMARFGDPVDPASVVVVSGSQQGLYLLARTLLEPGDV
                     VAVESPTYLGAIQVFRSVGARLLPIPVDEEGMRVELLEESIGRRRPKLIYTLPTFQNP
                     TGATLSLERRRRLLALAARHQVPIIEDDPYGALRYEGRSLPSLHALDEAGTVIYLSTI
                     SKMLFPGFRIGWLAAPRPVIERLGLMKQIVDLDTNPLAQWAVWAFLERGLLVEHVERL
                     RAVYPQRRDQMLAALARACGGALELGRPEGGFYLWGRLTGGLRSRDLLPQAARRGVAF
                     APGAPFHLDGGGEATLRLNFTLPDEAAIEVGVARLAAALAALGAGQDGFGERRVAAAT
                     PIV"
     gene            complement(2733..3056)
                     /locus_tag="VFU72_06800"
     CDS             complement(2733..3056)
                     /locus_tag="VFU72_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5423229.1"
                     /translation="MIVTTTNNVEGRAVREYLGIVSGEAILGANIFRDIFAGIRDVVG
                     GRSGAYEQELRKARDIAVAEMSEAAQQLGADAVVGVDIDYETIELGNHGGMMMVTAAG
                     TAVKL"
     gene            complement(3216..3932)
                     /locus_tag="VFU72_06805"
     CDS             complement(3216..3932)
                     /locus_tag="VFU72_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423230.1"
                     /translation="MASAVQQGVTSTSRPESRFPGDVITGICMIVGPVILVITSVLAI
                     GIYHAAGIDYVRGMAAHHTRTAAEFDLFIAGWMFLIFALIGLANRITAVRPWLGRAAG
                     IVSIVGLFGPVFFNGVYFGGFQLTGPSRQTAAGYLIDHAQIIPSNVINISGPALVIGF
                     ILLALGAAKAGVLGRPRAVALGLSCVMSAGFISGYMIIAVVAFLGMALALVPLGVSLL
                     RSHPAREGSLAPTRRQAAQG"
     gene            3988..4614
                     /locus_tag="VFU72_06810"
     CDS             3988..4614
                     /locus_tag="VFU72_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423231.1"
                     /translation="MAESRPYQSERRRRQAAETRRDILQAARRLFAERGYAATPLSEV
                     AAAAGVSVPTLYASVGAKADIARALVEFVNEEAEIAEHDRRQRVAETAPELIRANVHL
                     VRVLNERCGDIIRAMASAAASEPEVVPALEAGRRYHREGEQAIARRLGEMGALRAGLD
                     EAEAAAISSTLLSYEVFALFALTEGWSYDRIEDFLAGTLSGLLLRPGA"
     gene            4755..>5704
                     /locus_tag="VFU72_06815"
     CDS             4755..>5704
                     /locus_tag="VFU72_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU5423232.1"
                     /translation="MPEITLRDLCRWERRLALIPPVGVEREAALDRGLSWAVSVRAAP
                     PHLPPLRGEELVVLPLRVLEQLEAGESLTRQELLAELARQPIAAILTEPGFSEEPIDH
                     LPLLTLPAPFPLDAEGTLNRMITERRAELYRLGAELSRRLSQAVADPRGVEALLASAA
                     ELAGRDLLLEDTEGNVVAWSGAEQPAPSFALLGAGGARALDGPLLAATDARGREHLIV
                     ALSGGPSPGYLSLSGETGKLTEGDRLILTQTAVTCGALLGQSPRRTGGRDGRERLVAD
                     LLLGRLASSAAAQARARTLGIDGTQPVVVGLAAAPREADAR"
BASE COUNT          862 a         2050 c         1972 g          820 t
ORIGIN      
        1 tcccccccgc cccttctccc aatgctggga gaaggggggc aactaccgaa tacgtcgtcg
       61 tgctccctct cccgcgcttc gggagagggt ggggtgaggg ccctccgggt gtggagcgag
      121 ggcagtccgc ctcgcctacc agccgcgctt ggccagctgc tgctcggccg ggatcgacgc
      181 cagctccaga ccgcgcatcg cctcgcccag accacggctg acctcggcca gcaccttcgg
      241 attgcggaag tgggtcgtcg cctcgacgat cgccttgccg cgctcgtagg ggttgtccga
      301 cttgaagatg ccggagccga cgaagttgcc ctcggcgccg agctgcatcg tcaacgaggc
      361 atcggccggg gtggcgatgc cgccggcgca gaacatgacc accggcagct tgccggtctc
      421 ggccacctct accaccaggt cgtagggcgc gcccagctcc ttggccaccg acatcagctc
      481 ttccttcggc agcgactgca ggcgccggat gccgtcggtg atctcgcgca ggtggcgcac
      541 ggcctcgacg atgttgccgg tgccggcctc acccttgctg cggatcatgg ccgcaccctc
      601 gccgatgcgg cgcagcgcct cacccagatc gcgggcgccg cagacgaacg gcaccttgaa
      661 ctcgtgcttg ttgatgtgga agcgctcgtc cgccggggtc agcacttcgc tctcatcgat
      721 gtagtcgacg ccgatctcct ccagaatctg cgcctcggcg aagtggccga tgcgcgcctt
      781 ggccatcacc gggatggtga ccgcctcttt gatctggatg atcagctcgg ggtcgctcat
      841 gcgggccacg ccgccctgcg cccggatatc ggccggcacg cgctccagcg ccatcaccgc
      901 acaggcgccc gcctcctcgg cgatgcgcgc ctgctccgcc gtcaccacgt ccatgatcac
      961 gccgcccttg agcatctgcg ccaggccggc tttcgtcgtc caggtcgatt tctccaccgt
     1021 acgccctcca taggtttcca acccgcccag cccagtcgcc gggtgggacc aagcgctaga
     1081 caatcggcgt cgccgccgcg acgcgtcgct ccccgaagcc gtcctggccc gcgccgagcg
     1141 cggccagggc cgcggccagc cgggccacgc ccacctcgat cgccgcctcg tcggggagcg
     1201 tgaaattgag gcgcagcgtc gcctcgccgc cgccgtccag gtgaaacggc gcgccgggcg
     1261 cgaaagccac cccgcgccgc gccgcctgcg gcagcaggtc acgcgagcgc aggccgccgg
     1321 tcagccggcc ccagaggtag aagccgccct cggggcggcc cagctccagt gcgccgccgc
     1381 acgcgcgcgc cagcgcggcc agcatctggt cgcgccgctg cggataaacc gcgcgcaggc
     1441 gctcgacgtg ctccaccagc agcccacgct ccagaaaggc ccagaccgcc cactgcgcca
     1501 gcgggttggt gtccaggtcg acgatctgct tcatgagccc caagcgttcg atcaccggcc
     1561 ggggcgcggc cagccagccg atgcgaaagc cggggaagag catcttcgag atagtgctga
     1621 ggtagatcac cgtgccggcc tcgtccagcg cgtgcagcga ggggagcgaa cgcccctcgt
     1681 agcgcagtgc gccgtaagga tcgtcctcga tgatcggcac ctggtgccgc gccgccagcg
     1741 ccagcagccg gcgccgtcgc tccagactga gcgtcgcgcc ggtcgggttc tggaaggtcg
     1801 gcagggtgta gatcagcttg ggccgacgcc ggccgatcga ctcctccagc agctcgacgc
     1861 gcatcccctc ctcgtcgacc gggatcggca gcagccgcgc tccgaccgag cggaagacct
     1921 ggatggcgcc gaggtaggtg ggggactcga ccgccaccac gtcgcccggc tccagcagcg
     1981 tgcgcgccag caggtacagc ccctgctgcg agccggagac caccaccacg ctggccgggt
     2041 cgaccgggtc gccgaagcgg gccatccaat cggccagcac ctcgcgcagc ggcatgtagc
     2101 cctcggtcgg gcagtactgc aggagcgact gccccgactg gtgcagcgcc tcatccagca
     2161 gcgagcggat ctgctcgatc gggtagaggt cgggcgaggg gacgccggag gcgaacgaga
     2221 tcacgtccgg ccgcgccgcg acgagctgca cgtcagccag cagcggatcc tgcgccacct
     2281 cggtggctgc ggtgaagagc tgctcccagg gaagccgtgc cggtgaccgg cccagcgcca
     2341 gcgccggctc gtcggggacc gccagcagct cgcccggctg cgccactcgc gtgccctggc
     2401 cgacccggct gatgatcagc ccgtcgccgg ccagttcgcg gtaggcgttg acgaccgtgg
     2461 tgcgattgac gccgagcatg gccgccagcc gccgctccgg cggcagacgg taaccgggtg
     2521 tgagcgaacc gccgcgaatc tgctcgcgca gttggcggct gatctgtatg tagagcggcg
     2581 tctcgtccgc gcggtccagc tcgaccagca tccgatcctc cctttggatc cctccaataa
     2641 ggataccatc gggatccgaa ggaggaaacg gccaattgga tggggtttga agccatccaa
     2701 ttggcagaga gagctgagag ggacgcttgc ggctagagct tcacggccgt gccggcggcg
     2761 gtgaccatca tcatgccgcc gtggttcccc agctcgatcg tctcgtagtc gatatcgacg
     2821 ccgacgaccg cgtcggcccc cagctgctgg gctgcctcgc tcatctccgc cacggcgatg
     2881 tcgcgcgcct tgcgcagctc ctgctcgtag gcgcccgagc ggccgcccac gacgtcgcgg
     2941 ataccggcga agatgtcgcg gaagatgttg gcgccgagga tggcctcgcc gctgacgatg
     3001 cccaggtact cccgaaccgc ccggccctcc acgttgttgg tcgtcgtgac gatcatgtcg
     3061 acgctccttt cccggtcgcg cttcgcgggc aagcagcaca caacgattga cctcggggga
     3121 ccacacgtcc cggccactat cgtacacggg cggacgctgc gccggctgcg agagcaggaa
     3181 ggtgtcgccc acctccccgc ttcccgcacg cggtattatc cctgcgccgc ctgccggcgg
     3241 gtaggagcga gactgccctc cctggccggg tgcgaccgca gcaggctgac gccgagcggc
     3301 accagcgcga gcgccattcc caggaaggcc acgacggcga taatcatgta gccggagatg
     3361 aagccggccg acatgacgca ggagaggccg agcgcgacgg cgcgcgggcg gccgagcacg
     3421 ccggccttgg ccgcgccgag cgccagcaga atgaagccga tgaccagcgc cgggccggag
     3481 atattgatga cgttcgaggg gatgatttga gcgtgatcga tcaggtagcc ggcggcggtc
     3541 tgccgagacg ggccggtcag ttggaagccg ccgaagtaga ccccgttgaa gaagaccggg
     3601 ccgaagaggc cgacgatgct tacgatgccc gccgcgcgtc cgagccaggg acgcaccgcc
     3661 gtgatccggt tggccaggcc gatcagcgcg aagatcagga acatccagcc ggcgatgaag
     3721 aggtcgaact cggcggcggt acgcgtgtga tgcgccgcca tgccgcgcac gtagtcgatg
     3781 cccgccgcgt gatagatgcc gatggcgagc acggacgtga tgaccaggat caccggcccc
     3841 acgatcatac agatgccggt gatcacgtcg ccggggaacc ggctctccgg ccggctcgtg
     3901 ctcgtcacac cctgctgtac cgcgcttgcc atccgttggc cccttcctaa ttgaatccgg
     3961 ttataatcaa tactatagga tgatggcatg gccgagtcaa gaccctatca atcggagcgg
     4021 cggcggcggc aggcggccga gacccgccgc gacattctcc aggccgcgcg ccggctcttc
     4081 gccgagcgcg gctatgccgc cacgccgctg agcgaggtcg cggccgcggc tggtgtctcg
     4141 gtgccgacgc tctacgccag cgtcggcgcc aaggcggaca tcgcgcgtgc gctggtcgag
     4201 ttcgtcaacg aggaagcgga gatcgccgag cacgaccggc gccagcgggt ggccgagacg
     4261 gctccggagc tgatccgggc caacgtccac ctggtgcgcg tgctcaacga gcgctgcggc
     4321 gacatcatcc gcgccatggc ctcggcggcg gccagcgagc cggaggtcgt gccggccctg
     4381 gaggcgggcc gccgctatca ccgtgagggg gagcaggcca tcgcccgccg cctgggagag
     4441 atgggagcgc tgcgtgcggg tctcgacgag gcggaggcag ccgcgatcag cagcacgctc
     4501 ctctcctacg aagtcttcgc cctcttcgcc ctgaccgagg gctggtccta cgaccgcatc
     4561 gaggacttcc tggccggcac cctgagcggg ctgctgctgc ggcccggcgc atgatcctcc
     4621 cgcgcccgcg ccggactatt tggctcgcgg cgctacaatt atgtcgtgtt gatccaccat
     4681 agtgtacgta ccgggtcaac gccgcccgct atacttacga ccagggacca gccgaggccg
     4741 agtgagggag acgagtgccg gagatcacgc tgcgcgacct ctgccgctgg gagcggaggc
     4801 tggcgctcat cccgccggtc ggagtcgagc gcgaggccgc cctcgaccgg ggtctctcct
     4861 gggccgtctc cgtgcgcgcc gcgccaccac acctgccgcc cctgcgcggt gaggagctgg
     4921 tggtgctgcc gctgcgcgtg ctggagcagc tcgaagccgg tgagtcgctg acgcgccagg
     4981 agctgctggc cgagctggcg cggcagccga tcgcggcgat cctgaccgag ccgggcttca
     5041 gcgaggagcc gatcgaccac cttccgctgc tgacgctgcc tgcccccttt ccgctcgacg
     5101 ccgagggcac gctcaaccgc atgatcaccg aacggcgcgc cgagctctat cgcctgggcg
     5161 ccgagctctc gcgccggctc tcgcaggccg tagccgatcc gcgcggcgtc gaagcgctct
     5221 tggcctcggc cgccgaactg gccgggcgcg acctgctgtt ggaggacacc gagggaaatg
     5281 tggtcgcctg gagcggcgcc gagcagcccg cgcccagctt cgcgctgctc ggcgcgggcg
     5341 gcgcgcgcgc cctggacggc ccgctgctgg ccgcgacgga tgccaggggc cgcgagcatc
     5401 tgatcgtggc cttgagcggc ggtcccagcc ccggctacct ctcgctctcc ggtgagacgg
     5461 gcaagctgac cgagggtgac cggctgatcc tgacccagac cgccgtcacc tgcggcgcgc
     5521 tcctggggca atcgccgcgc aggacgggcg ggcgcgacgg gcgcgagcgg ctggtggccg
     5581 acctgctgct gggacggctg gcctcgtccg ccgcggcgca ggcgcgtgct cgcacgctgg
     5641 gcatcgacgg cacgcagccg gtggtggttg gcctggccgc agccccgcgc gaggcggacg
     5701 cacg
//
LOCUS       DASUBI010000353         7221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_327604, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000353 DASUBI010000000
VERSION     DASUBI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327604"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..178)
                     /locus_tag="VFU72_06820"
     CDS             complement(35..178)
                     /locus_tag="VFU72_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423233.1"
                     /translation="MPDSDPVTAGGSPRLFTLLRDARPVLFTLGEPGSFASTPPICGR
                     LAA"
     gene            complement(204..818)
                     /locus_tag="VFU72_06825"
     CDS             complement(204..818)
                     /locus_tag="VFU72_06825"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEU5423234.1"
                     /translation="MERSSIIQRRDLGVTVNANLPRAVVLVEGPSDEAALTTLASRRH
                     LDLAALGVAIVPMGGAHALSRFLDRFGPHGANIPVRGLYDAHEEPVVRHALAGAGLGS
                     RLTRAELERQGFYACDADLEDELIRALGTQAVEAIIAAQGELGPFRTFQRQPAQRTRA
                     LEQQLQRFLGTHAGRKIQYARLLVEALDLANVPRPLDRVLASVW"
     gene            complement(972..2438)
                     /locus_tag="VFU72_06830"
     CDS             complement(972..2438)
                     /locus_tag="VFU72_06830"
                     /inference="COORDINATES: protein motif:HMM:NF005303.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5423235.1"
                     /translation="MPGHAVVIVGGGPTGLMLAGELALAGVDVALVERRTSQDLVGSR
                     AGGLHARTIEVLDQRGIAERFLEQGQVAQVAGFAWIRLDISDFPTRHPYGLALRQQHI
                     ERILAGWVDEQAVPFYRGREVTGFAQDDTGVAVDLSEGQSLRADYLVGCDGGRSLIRK
                     AAGIEFPGWDPTISNLIAEVELAEEPEWGLRRDALGLHALSRLEDGERIGVMVTEQQL
                     GLTSEPTLRDLSETLSAVYGTDFGVHNPSWITRFTDMTRQAASYRAGRVLLAGDAAHV
                     HYPTGGQGLNIGLQDAVNLGWKLAQVVKGVAPESLLDTYHAERHPVAARVLRNTMAQV
                     ALLRPDERTDALRNSVSELLSMDEPRKRFGAMMSGLDIRYDLGEGHPLLGRRMPDLDL
                     LTSDGPLRIYTLLHAARPVLLNFGEPGSFDITPWADRVQLIDAHYAGVWELPVLGAVA
                     APPAVLVRPDGHVAWAGGLDGLGLPEALATWFGPPAPA"
     gene            3048..3743
                     /locus_tag="VFU72_06835"
     CDS             3048..3743
                     /locus_tag="VFU72_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423236.1"
                     /translation="MAVYLRVLRPWQLRWGATEDETIRPLPGDEVVPNPTFNATRAVT
                     IMARPEEIWPWLVQIGLRRAGWYSYDWIDNLGQPSAQRLILEFQQLAIGDFIPMSPIG
                     RVGQWVKEFEPNRWLLWWDNRGDVSWLWFLDPAGERHTRLITRVRMRYRWASPTILFN
                     LLLDVGDIVMMRKSLLGIKQRAERPAESPSVSRRHAPQACPFPAEGSQLAPRLKIAST
                     ASAIPSGKGAPRT"
     gene            complement(3740..3940)
                     /locus_tag="VFU72_06840"
     CDS             complement(3740..3940)
                     /locus_tag="VFU72_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423237.1"
                     /translation="MAKCDLVHRAFIKRLLQLAAVLLQGASGGWLGTRFVASSEWGGA
                     AQDQQAVLAAGTDDTVRTTVYD"
     gene            4095..7004
                     /locus_tag="VFU72_06845"
     CDS             4095..7004
                     /locus_tag="VFU72_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5423238.1"
                     /translation="MLSSSLGDEPALFVGRDRERGVLQARLTAALSGHGSLVLLSGEA
                     GIGKTALVEDLMQTAAGRDVLVLVGGCYDLTETPPYGPWFEALGDLPQQQDALGQPDQ
                     TRHVAPNRQPSIIAQVRAHLAALAADQPVVLVLEDLHWADPASLDVLRAVARDLERQP
                     LLLVVTYRDDEVTRHHPFYPLVPLLVREARAERMGLQPLDRAGLTTLVSRRYALHQHD
                     TAALVDYLARRSEGNPFFAGELVRLLEEMGELRRTEAGWLLDQLRVDAVPPLLRQVID
                     MRVDRLGEAARELLAAAAVIGETVPFGLWQAVSGADEVTLAEVVERAASARLMEESGD
                     GSGARFVHALIREALYEAIVPSRRRRLHRLAGESLVALPEPDADAVAHHFRAAGDERA
                     AAWLIAAGERAERAYAWMTAAERFEAALSLMATQRAGAGQRGWLLYRLATLRRFGDAP
                     RSVEALEDAGRLATEAGDLALAAYALVMLGHVRCFAGDLARGISELEAGIAAVAALPA
                     SEHARREVVDFVEPSTGTGTLVYWLPQVGRLAEARALGEEYVATSDARDAQGIARPSY
                     IDAFFGLGDVYARLGEPVRARQAYDDARAGYDALGYSHQSYTVLMNILHGVIVPYRTD
                     DLAERRRVTAEAEWGAARVRDIRGVLPSGIEQLPLMAVEAAGWDEARRLAGWVADRPA
                     AEQWSTEAVGAVLLARGDAEILEPLIAQELPQGPNSEPGSAMFESAIALQRAAATLAL
                     RAGDLPAAREWLEAHDRWLAWSGSVLGQADGALLWAEYFHHAGDAEQARRHAERALTH
                     AGAPRQPLALLAAHRLLGQIETTARRFAQAEMQLQASLALAQSCQAPFERALTLVELA
                     RLRLAQGQTDEARALLGEVRATCEPLGAGPALAQVAALEAQLAASRPARAFGLTEREL
                     DVLRRVAEGMTDAQVAEQLFISRRTVSSHLLSIYNKLGVPLRAAATRFAVEQQLL"
BASE COUNT         1098 a         2503 c         2501 g         1119 t
ORIGIN      
        1 agctggagac cgacctcgcc gcgtgattcg acgatcacgc ggcgaggcgc ccgcaaatcg
       61 gcggtgtgct cgcgaagctg cccggctcgc cgagcgtgaa gagcaccggc cgggcgtcgc
      121 gcagcagggt gaagagccgc ggcgagccac cggcggtcac tgggtcactg tccggcatgc
      181 ggcgcccgag cagccgatgg ctctcaccac acggacgcaa ggacccgatc aagcgggcgt
      241 ggaacgttgg cgaggtccaa cgcttccacg aggaggcgcg cgtactggat cttgcgcccc
      301 gcatgcgtcc ccaggaagcg ctgaagctgc tgctcgagcg ctcgggtgcg ctgtgccggc
      361 tgccgttgga aggtgcgaaa cggtcccagc tcgccctgcg ccgcgatgat cgcctccacc
      421 gcctgtgtgc cgagcgcccg gatcagctcg tcttccaggt cggcgtcgca ggcataaaag
      481 ccctgccgct ccagctccgc ccgcgtcagc cgggagccca ggcccgctcc tgccagcgcg
      541 tggcgcacga ccggctcctc atgcgcgtca tagagcccgc ggaccgggat gttcgcgccg
      601 tgcgggccga agcgatccag gaagcgcgag agggcatgcg cgccgcccat cggcacgatg
      661 gcaaccccaa gggccgcgag atccaggtgg cggcgagacg cgagcgtcgt gagcgcggct
      721 tcgtcgctcg gtccctcgac cagcaccacc gcccgcggaa ggttggcgtt caccgtgacc
      781 ccaagatcac gacgctggat gatcgacgac cgttccaatt tcggctcccg cgacggtgca
      841 agtgcgactc ctgaccgacg aagactggcc ggaatgactg gcatacatgc cccttcgcga
      901 cgtcgtgccg gaggacgggc cctgccgcgt gatacgtggg tcagacggcg aggtccacgg
      961 cgggcgtcgc gctacgccgg ggccggcggc ccgaaccagg tggctaaggc ctccggcagc
     1021 cccaagccat ctaggccgcc ggcccaggcg acgtgcccgt cgggccgcac cagcacggca
     1081 ggcggcgcgg ccaccgcgcc aagtaccggc aactcccaca caccggcata gtgcgcgtcg
     1141 atcagttgaa cgcgatccgc ccaaggcgtg atatcgaacg agccgggttc accgaagttg
     1201 aggagcaccg gccgggcagc gtgcagcagg gtgtagatcc gcagcgggcc gtcactggtg
     1261 agcaggtcga ggtcgggcat gcggcggccc agcagcgggt gtccctcgcc gagatcgtac
     1321 cggatgtcca gccccgacat catcgcgccg aaccgcttgc ggggctcgtc catgctgagg
     1381 agttcggaaa cgctgttgcg tagcgcgtcg gtgcgctcgt ccgggcggag caacgcgacc
     1441 tgcgccatcg tgttccgcaa gacgcgagca gcgaccgggt ggcgctcggc atggtaggta
     1501 tccaagaggc tctccggcgc tacccctttg accacctggg ccagtttcca gcccagattc
     1561 accgcatcct gcaagccgat gttgagaccc tgtccgcccg tggggtaatg cacgtgcgcg
     1621 gcatcgccgg ccagcagcac ccgccccgcg cggtaagaag ccgcctgccg ggtcatatcg
     1681 gtgaacctgg tgatccaact gggattgtgg actccgaagt cggtcccata gacggcgctg
     1741 agcgtctcgc tgaggtcgcg cagggtgggt tcgctggtga gcccgagctg ctgttcggtc
     1801 accatgaccc cgatccgctc cccatcctcc agcctgctca gggcatggag gccgagggcg
     1861 tcgcggcgca agccccattc cggctcctcg gccagctcga cctcggcgat caggttgctg
     1921 atcgtgggat cccagccagg gaactcgata ccggctgctt tccgaatcag gctgcgtccg
     1981 ccgtcgcagc ccaccagata gtctgctcgc aacgactggc cttcagacag atcgaccgcg
     2041 acgccggtgt cgtcctgcgc gaatccggtc acttcacggc cccggtagaa cggcacggcc
     2101 tgctcgtcga cccagccggc caggatgcgc tcgatgtgct gctgcctgag cgcgagcccg
     2161 taggggtgtc gggtgggaaa gtcgctgatg tccaaccgta tccaggcgaa gcccgccacc
     2221 tgcgccacct gcccctgctc caggaagcgc tcagcgatgc cgcgctggtc gagcacctcg
     2281 atggtgcgcg cgtgcaaccc accggcgcgc gaaccgacga gatcctggct ggtgcgtcgt
     2341 tcgacaaggg cgacatcgac ccccgctaac gccagctcgc ccgccaacat gagcccggtc
     2401 gggccgcctc caacgatcac caccgcatgc ccgggcatcg cgaccctcat cgttaccctc
     2461 ccatcggcat cacgccggtc gccaccagca cggcacaagt ctgcgccgct cgccaacatc
     2521 gagacctcgc tcgcccgtaa ccaccgagtc gtacgcacct cgcaggtctt accaaccgac
     2581 ggaaccggtc attctaccag ggatgtcgcg cccgggtgac gttcgagaac cgtctccggg
     2641 aagccgaagg tgtcatcgac gcgcgtgctc cgagcgcgcc gatcctcggg aatgcctggg
     2701 gggaagaaat caacaaagtt gtgatctggg gcaggcggtg gccgatcggg gtctgagcga
     2761 gtttccggtg aggcgaagca gtccagctcg ccctggtact gacggggccg ctgtggcgcg
     2821 tttccaccgt acctcgcacc accacgcatc ctacccattg gcgttgtcca tcccctcccg
     2881 ctgggtggtg cccagcacgc accgatagcc tatgctcgga caatttgggc gcgagccggg
     2941 ggtgctgacg gccccgtcga cccaacttca agcgcatcgc ctggtgagag gagaagcgca
     3001 tgctttcgcg cagacatttc ggcctggcaa tgcttggcgc cttcttcgtg gccgtgtacc
     3061 tgcgtgtcct gcgcccctgg cagctgcgtt ggggagcgac tgaggacgag accatccggc
     3121 cgctgccggg cgatgaggtg gtgccgaatc ccaccttcaa cgccacgcgc gctgtgacca
     3181 tcatggcgcg gccggaggag atttggcctt ggctggtgca gatcggcctg cgccgcgcgg
     3241 gctggtacag ctacgactgg atcgacaacc tgggtcagcc aagcgcgcag cgcctcatcc
     3301 tggaattcca gcagctggcg atcggggatt tcattccgat gtcgcccatc ggcagggtgg
     3361 gtcagtgggt aaaggagttc gagccgaatc gctggctgct ctggtgggac aaccgggggg
     3421 acgtgagctg gctgtggttc ctcgatccgg cgggcgagcg ccacacccgt ctgatcacgc
     3481 gcgtccgcat gcggtaccgc tgggcctcgc cgaccatcct cttcaaccta ttgctggacg
     3541 tgggcgacat cgtcatgatg cgcaagagcc tcctggggat caagcagcgg gcggaacgcc
     3601 ctgccgaatc gccgagcgtc tcgaggcggc atgcgccaca ggcgtgcccg tttccagcgg
     3661 aaggctccca gctcgccccg cgcctcaaga tcgcttccac cgcgtcggcg atcccctcgg
     3721 gaaaaggcgc gccgcgcacc tagtcataga cggtggtccg caccgtgtcg tcggtgcccg
     3781 cggccagcac cgcctgctga tcctgggccg cgccgcccca ctccgagctg gccacgaacc
     3841 gcgtccccaa ccagccgccc gacgctccct ggagcaagac ggcggcgagc tgcagcaatc
     3901 gcttgatgaa cgcgcgatgc accaagtcgc acttcgccaa gcgccgtacg atcgtttgct
     3961 tcaccgcacg ttgttctata atgaggtcca atcgtacgcc gtacccagca gaagacatcc
     4021 agcgcgaccg ccgtcgtaat ggcggcgtca cgaatgcttc acccgagacc ggtcgccgcg
     4081 acacgaggtg agccatgctg tcatcatcac tcggggatga gcccgcgctg ttcgtcgggc
     4141 gtgaccgcga gcgcggcgtg ctccaggcgc ggcttacggc agcgctgtcc ggccacggca
     4201 gcctggtgct gctcagtggc gaggccggca tcggcaagac ggcactggtc gaagacttga
     4261 tgcagacggc ggccggccga gatgtcctgg tgctcgtcgg tggctgctac gatctcacgg
     4321 agacgccgcc ctatggtccc tggttcgagg cgctcggcga cctgccccag cagcaggacg
     4381 cgctgggcca gccggaccag acgcggcacg tcgcgcccaa ccggcagccc tccatcatcg
     4441 cccaggtccg cgcccacctc gccgccttag cagcggacca gcccgtcgtc ctggtgctgg
     4501 aggatctgca ctgggccgac ccggcttcgc tcgacgtgct ccgtgccgtc gcccgcgacc
     4561 tggagcgcca gccgctgctc ctagtcgtga cctaccgcga cgacgaggtg acgcgtcatc
     4621 atcccttcta ccccttggtg ccgctgctgg tgcgcgaggc gcgagccgaa cgtatgggtc
     4681 tccaaccgct ggaccgggct ggcctgacaa ccctggtgtc ccgccgctac gctctccacc
     4741 agcacgacac ggccgcgctg gtcgattacc tggcgcggcg ctcggagggc aacccgttct
     4801 tcgcgggcga gctggtgcgg ttgctggagg agatgggtga gctgcggcgg acggaagcgg
     4861 gctggctgct cgatcagctt cgtgtcgacg ccgtgccgcc gctgctgcgc caggtgatcg
     4921 acatgcgcgt cgatcggctc ggagaggccg cccgcgagct gctggcggcg gcggcggtga
     4981 tcggcgagac ggtgccgttc ggcttgtggc aggccgtgag cggagcggac gaggtgacgc
     5041 tggccgaggt ggtggaacgt gccgcaagcg cgcgtctgat ggaggagtcc ggagatggga
     5101 gcggggcgcg cttcgtccat gcgctcatcc gtgaggcgct ctacgaggcc atcgtcccct
     5161 cgcgccggcg ccggctgcac cggctggccg gcgagtcgct ggtggcactc ccggagccgg
     5221 atgccgacgc cgtggcgcac cacttccggg ccgccggcga cgagcgagcg gcggcatggt
     5281 taatcgcggc gggagaacgc gccgagcggg cttacgcctg gatgacggca gcggagcgct
     5341 tcgaggccgc gctctccctc atggcgactc agcgagcggg cgcggggcaa cgcggctggc
     5401 tgctctaccg gctggccacc ctgcgccgct tcggggacgc gccgcgcagc gtcgaggcgc
     5461 tggaggacgc cggacggctc gcgacggagg cgggtgattt ggcgctggcc gcctacgcgc
     5521 tggtcatgct cggtcacgtg cgctgcttcg cgggcgattt ggcacgcggc atctcggagc
     5581 tggaggccgg tatcgcggcg gtcgcggccc tcccggcaag tgagcacgcc cgtcgagaag
     5641 tggtggactt tgtcgagcca tccaccggca cgggcacact cgtctactgg ctgcctcagg
     5701 ttggtcgctt ggcggaggct cgtgctctgg gcgaggagta cgtcgcgacc agcgacgctc
     5761 gcgatgccca aggcatcgcg cgtccttcgt acatcgacgc gttcttcggc ctgggcgacg
     5821 tctacgcgcg cctcggtgag cccgtgcggg cgcggcaggc gtacgacgac gcgcgcgcgg
     5881 gctacgacgc gctggggtat tcccatcaat cgtacaccgt cctgatgaac atcctgcacg
     5941 gcgtgatcgt gccctatcgc acggatgacc tggcggagcg gcgccgcgtg accgccgaag
     6001 ccgaatgggg agcggcgcgt gtgcgggaca tccgcggagt cctgccgagc ggcatcgagc
     6061 agcttcccct catggccgtc gaggcggccg gctgggatga ggcccggagg ctcgccggct
     6121 gggtggcgga tcgccctgcc gccgaacaat ggtcgaccga ggcggtcggc gcggtgctac
     6181 tggcgcgggg cgatgccgag atcttggaac cactcatcgc ccaggagttg cctcagggac
     6241 cgaacagcga gcccggctct gccatgtttg agtccgcgat cgctctgcaa cgggcggcgg
     6301 ccacactggc gctacgcgcg ggcgatctgc cggccgcgcg ggagtggctg gaggcgcacg
     6361 atcgctggct ggcctggagc ggctcggtgc tggggcaagc ggacggcgcg ctcctctggg
     6421 ccgagtattt tcatcatgcg ggcgatgccg agcaggcccg ccggcatgcc gagcgcgccc
     6481 tgacacacgc cggcgcccca cgccagccgc tggctctcct agccgcacac cgcctgctcg
     6541 gtcagatcga gaccactgcc cggcgctttg cccaggcaga gatgcagttg caggcgtccc
     6601 tggccctcgc ccagtcctgc caggcgccct tcgagcgggc cttgacgctg gtggagctcg
     6661 cacgcctgcg gttggcgcag gggcagaccg acgaggcgcg tgccctgctc ggcgaggtgc
     6721 gcgcgacctg cgaaccgttg ggcgccggtc cggcgctggc ccaggtggcg gcgctggaag
     6781 ctcagttggc ggccagccgg ccggcacgag catttgggct caccgagcgc gagctggacg
     6841 tgctgcggcg tgtggcggag gggatgaccg acgcccaggt ggccgagcag ctcttcatca
     6901 gccgccgcac cgtcagcagc cacctcctct ccatctacaa caagctcggc gtccccttgc
     6961 gcgccgctgc gacccgcttc gccgtcgagc agcagctgct ctgagccctg gcgagcgcgt
     7021 cgtcgtcctc ctggtaggca gtcagtagtt ctgcggacgc ccgaccctct gccgtctact
     7081 cgcggaatcc cgtagtccgc cggatgtgcc cgccccctgg ccgacctaga gtgatctggg
     7141 tcggcgaggc ggccagacgg agaagggggc cggggacgcg tccaccggca ccggtctaat
     7201 tagcgatggg agttcggcga t
//
LOCUS       DASUBI010000354         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_584070, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000354 DASUBI010000000
VERSION     DASUBI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584070"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU72_06850"
     CDS             <1..723
                     /locus_tag="VFU72_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5423239.1"
                     /translation="AASFAAFLLCNAYDQIRMGVAYPHLNVKLVGSHAGISIGEDGPS
                     QMGVEDVALACALPGMTVIVPSDEHQTRAATHAMLAHDGPVYLRVGRPAVPIIYDEQV
                     QFTIGKATQLRAGRDITLIADGIMVAGALDAAARLEQQGIQARVLDMATVKPLDTEAV
                     VAAARETGGIVVAEEHLHHGGLGSVVAMAVAANEPCRMAFVDLDDTFAQSGDAWELLQ
                     TYGLTGDRIYVAALDLVQQAKG"
     gene            773..1477
                     /gene="rpiA"
                     /locus_tag="VFU72_06855"
     CDS             773..1477
                     /gene="rpiA"
                     /locus_tag="VFU72_06855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU5423240.1"
                     /translation="MPDETEEEPAAREKRAASERAAAVVEDGMVVGLGSGTTAELAVR
                     ALGERVRDGLRIQGVPTSERTAALARSLGIPLVSLLHPGQVDLAIDGADEIVPGTLSL
                     LKGHGGALVREKLVAGAARRVLIVADHTKLVDRLGAHHSVPVEVVPFGARLVAVELMS
                     LGGQPVPRLVGLQPFITDNGNLLLDVAFGPIADSAALAARLKQISGVVDHGLFIGLAS
                     EALIGTSEGVRTLHAE"
     gene            1557..2906
                     /locus_tag="VFU72_06860"
     CDS             1557..2906
                     /locus_tag="VFU72_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5423241.1"
                     /translation="MIGEQQEPGESGLVSLYAMRLRSRAELGGARPPAGPAPEMRSFG
                     RPVSAPTRRRRVRVRRAAGVSALVATGLIVGTQAAAAAATTHQVEPGETLGGIAAHYH
                     VSVQQLTQLNHLNNPNLIIAGSTLTIDQDQAPAAAPAGAKQHQVLPGETLSTIASQYG
                     VTVDSVAKLNNLANPNVIVVGSELKIPAVGSSATQSASAPVDSGQVGLHLVVPGETLT
                     TIARSYGVQVRALAEANGISDLDFVEVGQMLRVPASLETGPVQSDGTVKLDSMPVMEQ
                     SLPLSCEDAAVSLATAYWGNQVSEWVFIENMPHNANPHLGFRGRLAGAFGGTDDYGVY
                     AEPFVSLLSRYGYTGDVFYAEGDAGQLKSQIDQGHPVVVWITNMASVQDKSYGYANGA
                     RFALVPQEHAVVVYGYDANGVFVADPGDGAYRHFNWGDFLRSWSYFDGMSLAVYPSA"
BASE COUNT          494 a         1101 c         1169 g          458 t
ORIGIN      
        1 gccgccagct tcgccgcctt cctgctctgc aacgcctacg accagatccg catgggcgtg
       61 gcctacccgc acctcaacgt caagctggtc ggcagccacg ccggcatcag catcggcgag
      121 gacgggccgt cgcagatggg ggtcgaggac gtggcgctgg cctgcgcgct ccccggcatg
      181 accgtgatcg tgcccagcga cgagcaccaa acgcgcgccg cgacgcatgc gatgctggcg
      241 cacgatggcc cggtctacct gcgcgtcggc cggccggcgg tgccgatcat ctacgacgag
      301 caggtccagt tcaccatcgg caaggcgacg cagttgcgcg cgggccgcga catcaccctg
      361 atcgccgacg ggatcatggt ggccggggcg ctggatgccg cggcgcggct ggagcagcag
      421 gggatccagg cgcgggtgct cgacatggcg acggtcaagc cgctggatac cgaggcggtg
      481 gtggcggcgg cgcgcgagac cggcgggatc gtggtggccg aggagcacct gcaccacggc
      541 gggctgggaa gcgtggtggc gatggcggtg gcagcgaatg agccctgccg catggccttc
      601 gtcgacctcg acgacacctt cgcgcagtcc ggtgacgcct gggagctgtt gcagacctat
      661 ggcctgaccg gcgaccgcat ctacgtggcc gcgctcgacc tggtgcagca ggcgaagggg
      721 tagggcgggg ggtgagggct cctcgtgagg gaccgtgcga aacggaggac tggtgcctga
      781 cgaaacagag gaggagcccg ccgcgcggga gaagcgcgcg gcgtccgagc gcgcggcggc
      841 ggtggtcgag gacggcatgg tggtcggcct cggcagcggc acgaccgccg aactggccgt
      901 gcgggcgctc ggcgagcggg tgcgcgacgg gctgcgcatt cagggcgtgc cgacctcgga
      961 gcgcaccgcc gcgctggccc gctctctcgg catcccgctg gtgtcgctgc tgcacccggg
     1021 gcaggtcgac ctggcgatcg acggcgccga cgagatcgtg ccgggcacgc tgagtctcct
     1081 caaggggcac ggcggagcgc tggtgcgcga gaagctggtc gccggcgccg cgcgccgcgt
     1141 gctgatcgtg gccgaccaca ccaagctggt cgaccggctc ggcgcgcatc attcggtccc
     1201 ggtcgaggtg gtgcccttcg gcgcgcggct ggtggctgtc gagttgatgt cgctcggcgg
     1261 ccagccggtg ccgcgcctgg tcgggctgca gcccttcatc accgacaacg gcaacctgct
     1321 gctcgacgtc gccttcggcc cgatcgccga ctccgcggcg ctggccgcgc gcctcaaaca
     1381 gatcagcggc gtggtcgacc acggcctctt catcgggctg gccagtgaag cgctcatcgg
     1441 caccagcgag ggcgtcagaa ccctgcacgc cgaatagccc cggcttgcca attcgtcacc
     1501 acccagctat actcgtgtac gtacagtatg tacgagcgag cgcaggggag gtcacgatga
     1561 tcggcgagca gcaggagccg ggcgagagcg gactggtgtc gctctacgcg atgcgtttgc
     1621 gcagccgagc ggagctggga ggggcgcggc cgccggccgg gccggcgccg gagatgcgct
     1681 cctttgggcg cccggtatcc gctcccacgc gtcgacggcg cgtgcgcgtg cggcgagccg
     1741 cgggcgtctc ggcgctggtg gcgaccgggc tgatcgtggg cacgcaggcg gccgccgccg
     1801 cggcgaccac gcaccaggtc gagcctggtg agacgctggg cggcatcgcg gcgcactacc
     1861 acgtcagcgt gcagcagttg acgcagctca atcacctgaa caacccgaac ctgatcatcg
     1921 ccggctcgac gctgacgatc gaccaggacc aggcgccggc cgccgcaccg gcgggcgcca
     1981 agcagcatca ggtgctgccg ggggagacgc tctcgaccat cgccagccag tacggcgtca
     2041 cggtcgactc cgtggccaag ctcaacaacc tggccaaccc caacgtgatc gtggtgggca
     2101 gcgagctgaa gatcccggcg gtcggctcga gcgccaccca gagcgccagc gcgccggtgg
     2161 actccggtca ggtggggctg catctggtgg tgccgggcga gacgctgacc accatcgccc
     2221 gcagctacgg agtccaggtg cgcgcgctag ccgaggccaa cggcatcagc gatctcgact
     2281 tcgtcgaggt cgggcagatg ctgcgcgtcc ccgccagcct ggagaccggg ccggtgcaga
     2341 gcgacggcac ggtcaagctg gacagcatgc cggtaatgga gcagtcgctg ccgctcagtt
     2401 gcgaggacgc ggcggtcagc ctggcgaccg cctactgggg taaccaggtc tccgagtggg
     2461 tcttcatcga gaacatgccg cacaacgcca acccgcacct gggctttcgc ggccggctgg
     2521 ccggggcctt cggcggcacc gacgactacg gcgtctacgc cgagccattc gtttcgctgc
     2581 tcagccgcta cggctacacc ggcgatgtct tttacgccga gggtgacgcc gggcagctca
     2641 agtcgcagat cgaccaggga cacccggtgg tcgtctggat caccaacatg gcttcggtgc
     2701 aggacaagag ctacggctac gccaacggcg cgcgcttcgc gctggtgccg caggagcacg
     2761 cggtggtggt ctacggctac gacgccaacg gcgtcttcgt cgccgatccc ggcgacggcg
     2821 cctaccggca cttcaattgg ggtgatttcc tgcgctcgtg gagttacttc gacggcatgt
     2881 cgctggccgt ctacccgagc gcctgacaca gcgcacaccc tccggggttg ggttgcgcgg
     2941 agacgcccgg cacggggaca gccgggcgtt tccgcttgcc cccggggacc gggagcggca
     3001 ggcacggtcc ttaatcgcgg cgcaccgctc gctgtagggg cagacctccg tgtctgccct
     3061 cgccgccctg ccaccggaca tagccgatta ccgcaccact gcccctattc cccgttgccg
     3121 agacgcgggg gagacagggc cgacacggag gcctgcccct acacggaatg cggattcgcc
     3181 ctcgccggga acactgccaa ttgttctatc ccggaaaagc gc
//
LOCUS       DASUBI010000355         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_211892, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000355 DASUBI010000000
VERSION     DASUBI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211892"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1024
                     /locus_tag="VFU72_06865"
     CDS             320..1024
                     /locus_tag="VFU72_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423242.1"
                     /translation="MNTKFFDELTVSLGTAKTRRSVARVLAGGAAAGALARLGVQAAA
                     ACTPLGKKCAHSQDCCFGGCKGHKCRCSAGNKPCNGRCIPVDQCCNGCPSGQVCQGGT
                     CVGEPRQTVEVATNMKIVDHDNWPGSDDICSPLKDSRKVTLTAANPRASFDSIVETCD
                     VQRVDFDLDAELLDSGHIRVTGIAHLSTNGSFADQVEIGPIDAASGVPAQVPFKLGAP
                     DGNRAEGTVTVRTSNA"
     gene            complement(1235..>2610)
                     /locus_tag="VFU72_06870"
     CDS             complement(1235..>2610)
                     /locus_tag="VFU72_06870"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423243.1"
                     /translation="EGPDAPLWHDRLTRDLDNLRAALDRLLDQRPADASLSVASALFR
                     FWSLGPHRQAGREYLRRALEQAGAESSPVAARTLLTAAHLANQAAAYQDATRLYEQSR
                     IMFESLGDVAGVAMVEIAHGMIELNLGQLETAFTWFTASRAHYEQAGDGAGVADAIGC
                     AGNARSAQGRLIEATALLEAAERRYRELGDDLGLAICRVDQADTLRDQGHPGEAAALL
                     EDSLDALRRYGNWHGLGLALTRLADVVREQGDLARSADLLDEAATLSTQVHSQGLAGW
                     ALLQRCRLGLAARDFAAAQQAGEQAIPLLDAGGARWESCEAQETLALVHAAQGEWEGA
                     RRLALESLIGFAAMGAQVWTVNVLETVIAILAATRPDPRLAAAGAAADSVRQTLRARR
                     LPTREARFARAMDILRAALGADGFAAAWERGATTSLAEAVAAVNALSESSTGINSGVS
                     NQATIIK"
BASE COUNT          425 a          899 c          880 g          406 t
ORIGIN      
        1 gacagcccgg ccgcgagtcg gtgttggcgg agtaatccgc cgaaagttgc cgatgacccg
       61 ggcttctccc gtgctcgctt cgtggaaacc atcaccccgc tcccgcgctt gcatggatcg
      121 taatccggga ccgtccgccg gtacaacgtc cgcccgctgt gggctgccgc ttcgtcactg
      181 cgtgggtagt gcgtggcaag cgcgtacaga gtgcgcgtag tggctggcgg ggctgggacg
      241 gaggatggag ccaagttcgg cgggggccgg acgacggaga tctggacaag ggctggcgcg
      301 tagccagcaa ggagccaaga tgaacaccaa gttcttcgac gagttgaccg tttccttggg
      361 caccgcgaag acgcgtcgga gcgtggcccg ggtcctcgcc ggcggagccg cggcgggcgc
      421 tctggccaga ctcggggtcc aggcggccgc cgcctgcacc ccgctcggca agaagtgcgc
      481 gcacagccag gactgctgct tcggcggctg caaaggccac aagtgccggt gcagcgccgg
      541 caacaagccg tgcaatgggc gctgcattcc ggtcgaccag tgctgcaacg gctgcccctc
      601 cggccaggtg tgccaaggcg ggacctgcgt cggggagccc cggcagaccg tcgaggtggc
      661 aacgaacatg aagatcgttg atcacgacaa ttggccgggc agcgacgaca tctgctcgcc
      721 gctcaaggac tcccgtaagg tcacgctgac ggccgccaat ccccgggcaa gcttcgacag
      781 catcgtggag acctgcgacg tgcaacgcgt cgacttcgat ctcgacgcgg agctgcttga
      841 cagcggccac attcgggtga cgggcattgc gcacctctcc accaatgggt cgttcgcgga
      901 tcaggtagaa attggaccga tcgatgccgc ctctggggtc cccgcccaag tcccgttcaa
      961 attaggcgct ccggatggca accgcgctga aggcacggtg acggtccgca cctccaacgc
     1021 ctagcgggtg gggcaccccc acttggcatc gcgggaagca acggcaacgg gtcgcgcgcc
     1081 gggggctcaa gttgggctcc cggcgccggt ctgagttgag gggactgccc ccgcagcatg
     1141 tgcggccgag gtcattggcg gcgacgattt cgtctccagg ggcgaccgcg acggactctg
     1201 ttgctgcggt gatcgcctca gaaccaacgg ttcactattt gatgattgtc gcttgattcg
     1261 agacgccgga attgatgccg gtcgacgatt cactcaacgc attgaccgct gcgaccgcct
     1321 cagcaaggga tgtcgttgcc cccctttccc atgccgctgc aaagccatcc gcccccagcg
     1381 cagcgcggag gatgtccatt gcccgcgcga atcgcgcctc ccgcgtcggc agccgccgcg
     1441 cccgcaaggt ctgtctgacg gaatctgctg ccgcgcccgc cgccgccaga cgcggatccg
     1501 gacgcgtcgc cgcgaggatc gctatgaccg tctccagcac gttgaccgtc cagacctgcg
     1561 cccccatggc ggcaaacccg atcaggcttt cgagcgctag gcggcgcgcc ccctcccact
     1621 cgccctgcgc cgcgtgcacg agcgcgagag tttcctgggc ttcacaggat tcccagcgcg
     1681 ccccacctgc gtcaagcagg ggaatggcct gctcgccggc ctgctgcgcc gcagcaaagt
     1741 cacgggcggc gaggccgagg cggcagcgtt gcagcagcgc ccaccccgcc aggccttggc
     1801 tgtgcacctg cgtgctgagc gtggcagcct cgtccagcag gtctgcggaa cgggcaaggt
     1861 ccccctgttc gcggaccacg tccgccaagc gggtgagggc gaggccaagc ccatgccaat
     1921 tgccatagcg ccgcagggcg tcaaggctgt cctccaggag cgccgccgcc tcgccgggat
     1981 gaccctgatc gcggagggtg tcggcctgat cgacgcggca aatcgccagc cccaggtcgt
     2041 cgccgagttc ccgatagcgc cgctcggccg cctcgagcaa cgccgtcgcc tctatcagcc
     2101 gaccttgcgc cgagcgggcg ttcccggcgc acccgatggc gtccgccacg ccggccccgt
     2161 cgccagcctg ttcatagtgc gcacggctcg ccgtaaacca agtgaacgcg gtttcgagct
     2221 ggcccagatt gagctcgatc atgccgtgcg cgatctcaac catcgcgacg cccgccacgt
     2281 cgccgaggga ttcgaacatg attcggcttt gctcgtacag ccgggttgca tcctggtatg
     2341 cggcggcctg gttcgcgaga tgggccgccg tcagcaaggt gcgggccgcc acgggactcg
     2401 actccgcgcc ggcctgctcg agggcacggc gaagatactc gcgtcccgct tgccgatggg
     2461 gaccgagcga ccaaaagcgg aagagggcgg acgcgaccga aagactggcg tccgccggtc
     2521 tctggtcgag caatcggtcg agcgcggcgc ggaggttgtc gaggtcacgc gtcagtcggt
     2581 cgtgccacag cggcgcgtcc ggtccctcca
//
LOCUS       DASUBI010000356         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_707664, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000356 DASUBI010000000
VERSION     DASUBI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707664"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFU72_06875"
     CDS             complement(<1..1724)
                     /locus_tag="VFU72_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5423244.1"
                     /translation="MVLSRMRGARVIRKEDPRLITGSATYVDDVRVPGTLHMVFARSQ
                     HPHARIRSIRTDQASQMPGVVAIYVGGKANASGNQHVRVLRDIVAPSEEPAEEVEAPD
                     IRETEEAEHPEEAREPEQFWMAIDTVRSVGIAVAGVVAETEEQARDAAEAIEIDYEPL
                     PAVVDVEAAMKDGAPQLYPQFANNICHHYDHTAGDADAAFKAAPITVKERLINQRVAG
                     VPMEPRGVLAMPDPMINGLTFWTSTQAQFWNRRDVAQSLNIPESNVRTIAPQVGGGFG
                     VKIGAYPEDYILSAVARDLGRPVKWIETRSESLVVTHQGRAQVADVEVAAEQNGKITG
                     YRLNVIQDQGFYPKDVSLSTLTGLMAVGCYDIKNIDVHAIGVLTNTTEIGAYRGAGRP
                     EAAFYIERVVDLVADACKLDPAEVRRVNFIQPNQFPYATVTGAKYDSGDYEKALDKAL
                     QNVNYKQLRQEQADARKQGRYIGIGLSSYVEICGFGPFDSAIVRVERTGAVTVYSGIL
                     PQGQGQETAFAQIVADDLGVPFDSISVRFGDTASSPQGQGTMGSRGLVVGGSAVVGAA
                     AQVREKAKR"
     gene            complement(1828..2313)
                     /locus_tag="VFU72_06880"
     CDS             complement(1828..2313)
                     /locus_tag="VFU72_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5423245.1"
                     /translation="MRRHAVAGRLRVGPADDIGALTPAMTEETTVFSRAVHLAPLPLR
                     IGLGIIMVSHGQPKVFGESKEHFPGTVESLGVPAPEKMARLVSYLELFGGLALIAGFL
                     TRLIAFLFVGEFGFVILRVKWSKGLMQGYEFDLSLLAGFLSLVLLGAGAGSLDRLLGI
                     D"
     gene            2397..2777
                     /locus_tag="VFU72_06885"
     CDS             2397..2777
                     /locus_tag="VFU72_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423246.1"
                     /translation="MARVYQLEHVGLGARRDLYETTVRFYEEVFGWHRLRELPGELTF
                     VGDGAGARLEIFPTDSAALPNPHHLAFGVDLDQFDAMRETLRQAGATLDEPYVNEYGD
                     RIGYFIDPAGNRAQIVGRKAPLPR"
BASE COUNT          445 a         1053 c         1048 g          524 t
ORIGIN      
        1 cgcttggcct tctcccgcac ctgcgccgcc gcgccgacga ccgcgctgcc gccgaccacc
       61 aggccgcggc tgcccatcgt cccctgaccc tgcggcgagg aggcggtgtc gccgaagcgg
      121 accgagatcg agtcgaaggg cacgcccagg tcatcggcca cgatctgggc gaaggcggtc
      181 tcctggccct ggccctgggg caggatgccg ctgtagaccg tgacggcgcc ggtgcgctcg
      241 acgcgcacga tggcgctgtc gaaggggccg aagccgcaga tctcgacata ggaggagagg
      301 ccgatgccga tgtagcggcc ctgcttgcgc gcgtcggcct gctcctggcg cagctgcttg
      361 tagttgacgt tctgcagcgc cttgtcgagc gccttctcgt agtcgccgct gtcgtacttg
      421 gcgccggtga cggtggcgta ggggaactgg ttgggctgga tgaagttgac gcggcgcact
      481 tcggcggggt cgagcttgca ggcgtcggcc accagatcga ccacgcgctc gatgtagaag
      541 gcggcctcgg ggcggccggc gccgcggtag gcgccgatct cggtggtgtt ggtcagcacg
      601 ccgatggcgt ggacgtcgat attcttgatg tcgtagcagc cgacggccat cagtccggtc
      661 agcgtagaca ggctgacgtc cttgggatag aagccctggt cctggatcac gttcagccgg
      721 tagccggtga tcttgccgtt ctgctcggcg gcgacctcga cgtcggctac ctgggcgcgg
      781 ccctggtgcg ttaccaccag gctctcgctg cgcgtctcga tccacttgac cgggcggccg
      841 agatcgcgcg ccacggcgct gaggatgtaa tcctcggggt aggcgccgat cttgacgccg
      901 aagccgccgc cgacctgcgg cgcgatggtg cgcacgttcg actcggggat gttgaggctc
      961 tgggccacgt cgcgccggtt ccagaactgc gcctgggtgg aggtccagaa ggtgagcccg
     1021 ttgatcatcg ggtcgggcat ggccagcacg ccgcgcggct ccatcggcac cccggccacg
     1081 cgctggttga tcagccgctc cttgacggtg atcggggccg ccttgaaggc ggcgtcggcg
     1141 tcgccggccg tgtggtcgta gtggtggcag atgttgttgg cgaactgcgg atagagctgc
     1201 ggcgcgccgt ctttcatggc cgcttcgacg tcgaccaccg ccggcagcgg ctcgtagtcg
     1261 atctcgatcg cctcggcggc gtcgcgcgcc tgctcctcgg tctcggccac gacacccgcc
     1321 accgcgatac cgacgctgcg cacggtgtcg atcgccatcc agaactgctc gggctctcgc
     1381 gcctcctcag gatgctccgc ctcttccgtt tcgcggatgt ccggcgcctc gacttcctcg
     1441 gccggctcct cactcggcgc cacgatgtcg cgcagcacgc gcacgtgctg gttgccgctg
     1501 gcgttggcct tgcctcctac gtagatggcg acgacacccg gcatctggct ggcctggtcg
     1561 gttcggatgc tgcgtattct ggcgtgcggg tgctggctgc gcgcgaagac catgtgcagc
     1621 gtgcccggca cgcgcacgtc gtcgacgtag gtcgccgagc cggtgatcag gcgcggatcc
     1681 tccttgcgaa tgacccgagc gccccgcatt ctgctgagta ccatcccgtc acctccgctg
     1741 gagccttgtc gttggccggc gccgcgcgcc agtctgatcg ttggtctatt gtcgcctgct
     1801 tctggccggg cggcaatcct gccgcgctca gtcgatgccc agcaggcggt cgagcgagcc
     1861 ggcgccggcg cccagcagca ccaggctgag gaagccggcc agcagcgaga ggtcgaactc
     1921 atatccctgc atcagacctt tggaccactt cacgcgcagg atgacgaagc cgaactcgcc
     1981 gacgaagagg aaggcgatca accgggtgag gaatccggcg atgagcgcca gcccgccgaa
     2041 gagctccaga taactcacca accgcgccat cttctccggc gcgggcacgc cgagcgactc
     2101 caccgtgccg gggaagtgct ccttgctctc gccgaagacc ttcggttgcc cgtgcgacac
     2161 catgatgatc cccagcccga tccgcaacgg gagcggcgcc agatgaacgg ccctcgaaaa
     2221 cacagtcgtc tcctctgtca ttgccggcgt cagcgcgccg atatcgtccg ccggcccgac
     2281 ccgcaaacgc ccggccacgg cgtggcggcg cacgatcctt cccggcgtcc tctgtacgtg
     2341 ctacaagtgg cgtgcggaac agcgttgctt gagaaacgtc ggaggaggag ccagccgtgg
     2401 ccagggtcta tcaactggag cacgtgggac tgggggcgcg ccgcgatctc tacgagacga
     2461 cggtgcgctt ctacgaggag gtcttcggct ggcaccgcct gcgcgagctg ccgggcgaat
     2521 tgaccttcgt cggcgacggc gccggcgcgc gtctggagat cttcccgacc gactcagcgg
     2581 ccttgcccaa cccgcaccac ctggccttcg gcgtcgatct ggaccagttc gacgccatgc
     2641 gcgagacctt gcgccaggcc ggcgccacgc tcgacgagcc gtacgtcaac gaatacggcg
     2701 accgcatcgg ctacttcatc gacccggccg gcaatcgcgc ccagatcgtc ggacgcaagg
     2761 cgccgctgcc gcggtagggt cgttgcatac ctttcgcacg gggcgaatgc gccggtcgcg
     2821 gtaggggcag acctctgtgt ccgccctcgt tgccctattg accatctgct cgtccgtcgt
     2881 cccacgttgc ccatgtcccc gttgcctcgc tgtaggggaa agagggcaga cacggaggcc
     2941 tgcccctaca gtgcgtgagg cacggcggtc agcgacggcg tgagccgcgc tacggcccgg
     3001 gcggcagcag gtccgccacg tcgtccaggc ggaagaccca gatctcgggg ccgctgtagc
     3061 gatcgccgcg
//
LOCUS       DASUBI010000357         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_330043, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000357 DASUBI010000000
VERSION     DASUBI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330043"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFU72_06890"
     CDS             complement(<1..584)
                     /locus_tag="VFU72_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5423247.1"
                     /translation="MDRLWTPWRMRYVGGETRAPGCIFCTKPADQNDITNLILYRGSD
                     AFIIMNLYPYNTGHVMVVPYAHAAGLDQLTPAEVGGTFGLLPWVTAAQRRALRCEGIN
                     IGLNLGRVAGAGVAEHLHVHVVPRWEGDANFMPILANTMVLPELLPVTYAKLRAELAV
                     SALGMPPGEQLLPQAGAVVLLPSLGQIALRRSADG"
     gene            788..2269
                     /locus_tag="VFU72_06895"
     CDS             788..2269
                     /locus_tag="VFU72_06895"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEU5423248.1"
                     /translation="MATLAGVLTRQSARAQRFGRLIAAGRAVSDASLILLAFVAGYWL
                     RYRWEIGGQVLPGDVRSFGFFIGKAVMLMVATVAIFQLRGLYRLPRWTGLLDEASVIA
                     SGATTGMALVILYSFLQRFYPSRLIFIYTWLLVIVFLLGKRLVTRQLRGALWRRGIGV
                     DRVLVVGAGRAGQRYMQQILNSPLAGYQMVGFVHDEAIEDNWGIATERRVERPPYLGT
                     IDRVPELVRQYEADEVVIALPPNAHAQVHRLIAQCRESDVAFTLVPDLFDLALDHVVT
                     HDLGGLPLITLNESRIRGWNYGLKRAMDIVIASAILVSLSWLMALIALAIRLDSPGPV
                     FFTQERVGKGRRTFILYKFRTMHRDAEREKADLQAQYNVGDLLFKIPDDPRRTRVGRV
                     LRRTSLDELPQFINILLGDMSVVGPRPQVPEEVAKYEEWHYHRLEVTPGLTGLWQVSG
                     RSDLSFDEMVKLDLYYAEHWSPWLDIKLILRTIPAVILGRGAY"
     gene            complement(2263..>2859)
                     /locus_tag="VFU72_06900"
     CDS             complement(2263..>2859)
                     /locus_tag="VFU72_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423249.1"
                     /translation="EQARRLFLEQGYAAVSMQQIADAAGVNKATLYHHFRDKEDLFLA
                     VFGAELARIEGAVREALTDAGTLRQRLQRVAAGFLASPDCDVGRLLAELKRHVGEERR
                     AELHRRVRFPWEVLLPVLQASAAELRVEPELVAELFFGMLVSQARRAKLNQQPPRPDL
                     ARVIVDILLDGAGAQGTAGADAGDSRLTNAMSLALPPQ"
BASE COUNT          435 a         1007 c          963 g          454 t
ORIGIN      
        1 ccgtccgccg agcggcgcag cgcgatctga cccagcgagg gcagcagcac gaccgcaccg
       61 gcctgcggca ggagctgctc gcccggcggc atcccgagcg cggagacggc cagctcggcg
      121 cgcagcttgg cataggtgac cggcagcaac tcgggcagca ccatggtatt ggccaggatc
      181 ggcatgaagt tggcgtcgcc ctcccagcgc ggcacgacgt ggacgtgcag gtgctcggcc
      241 acgcccgccc cggccacgcg gccgagattg aggccgatgt tgatcccctc acagcgcagc
      301 gcgcgccgct gggccgcggt gacccacggg agcagcccga aggtgccgcc gacctccgct
      361 ggtgtgagct gatccagccc ggcggcgtgc gcatagggca ccaccatcac gtgaccggtg
      421 ttgtaggggt agaggttcat gatgatgaac gcatcgctcc cccggtagag gatcaggttc
      481 gttatgtcgt tctggtcggc cggtttggta cagaatatgc atccgggggc gcgagtctcg
      541 ccgccgacgt agcgcatgcg ccacggcgtc cacaggcggt ccatgctgcg gtgatcctcg
      601 gcctgctcac ccatatctcc cccgatctta gcacagggct gacagccggc ttttggcccg
      661 aaagtccggg gagccggcgc caagcgggct agtttgatga acggacactc tcggctgcta
      721 tacttcggcg tcaactgccg cgccgagagg gggacacgag gcaatggcgg ttcgttcggg
      781 aggggacgtg gcgaccctgg cgggggtgct tacccgacag agcgcccgcg cgcagcgctt
      841 cgggcggctg atcgccgccg gccgcgccgt ctccgatgcc agcctgatcc tgctcgcctt
      901 cgtggccggc tactggctgc gctatcgctg ggagatcggc ggccaggtgc tgccgggcga
      961 tgtgcggtcc ttcggcttct tcatcggcaa ggccgtcatg ttgatggtgg ccacggtcgc
     1021 catcttccaa ctgcgtgggc tctaccgcct gccgcgctgg accggcctgc tcgacgaggc
     1081 ttcggtcatc gccagcggcg ccaccaccgg catggcgctg gtgatcctct acagcttcct
     1141 gcagcgcttc tacccgtcac gcctgatctt catctacacc tggctgctgg tgatcgtctt
     1201 cctgctcggc aagcgcctgg tgacccgcca actgcgcgga gcactctggc gccgcggcat
     1261 cggcgtcgac cgcgtgctgg tcgtcggcgc cgggcgcgcc ggccagcgct acatgcagca
     1321 gatcctcaac tcgccgctgg ccggctacca gatggtcggg ttcgtccatg acgaggccat
     1381 cgaggacaac tggggcatcg ccacggagcg gcgcgtcgag cgcccgccgt atctcggcac
     1441 gatcgaccgt gtgcccgagc tggtgcgcca gtacgaggct gacgaggtgg tcatcgcgct
     1501 gccgccgaac gcgcacgccc aggtgcaccg cctcatcgcc cagtgccgcg agagcgacgt
     1561 cgccttcacg ctggtgcccg atctcttcga tctggcgctc gaccacgtgg tgacgcacga
     1621 cctcggcggg ctgccgctga tcaccctcaa cgagtcgcgt atccgcggct ggaactatgg
     1681 cctcaagcgc gccatggaca tcgtcatcgc cagcgctatt ctggtctcac tctcctggct
     1741 gatggcgctg atcgcgctgg cgatccggct cgactcaccc ggcccggtct tcttcaccca
     1801 ggagcgcgtc ggcaaggggc ggcgcacctt catcctctat aagttccgca ccatgcaccg
     1861 cgacgccgag cgcgagaagg ccgacctgca ggcgcagtac aacgtcggcg atctcctctt
     1921 caagatcccc gacgacccac ggcgcacccg cgtcggccgc gtcctgcgtc gcaccagcct
     1981 ggacgagctg ccacagttca tcaacattct gctgggcgac atgagcgtgg tcggcccgcg
     2041 gccgcaggtg ccggaggagg tcgccaagta cgaggagtgg cactaccacc ggctggaggt
     2101 cacgccgggg ctgaccgggc tgtggcaggt cagcgggcgc agcgacctct ccttcgacga
     2161 gatggtcaag ctcgacctct actacgccga gcactggtcg ccctggctcg acatcaagct
     2221 gatcctgcgc acgatccccg cggtgatcct gggccgcggc gcctactgag gcggcagcgc
     2281 gagcgacatg gcgttcgtca gccgcgagtc gccggcgtca gcaccggcag tgccctgggc
     2341 accggcgccg tcgagcagaa tgtcgacgat gacgcgcgcc agatcgggcc ggggcggctg
     2401 ctggttcagc ttggcgcgcc gcgcctggct gacgagcatg ccgaagaaga gctcggccac
     2461 cagctccggc tcgacgcgca attcagcggc cgatgcctgc aagaccggca gcagcacctc
     2521 ccaggggaag cgcacgcggc gatgcagctc ggcgcgccgc tcctcgccga cgtggcgctt
     2581 cagctccgcc agcaggcggc cgacatcgca gtcgggcgag gccagaaacc cggcggcgac
     2641 gcgctgcagc cgctggcgca gcgtgcccgc gtcggtcagc gcctcgcgga ccgcgccttc
     2701 gatacgcgcc agttcggcgc cgaagacagc caggaacaga tcttccttgt cgcggaaatg
     2761 gtggtagagg gtcgccttgt tcaccccggc ggcgtcggcg atctgctgca tcgacaccgc
     2821 ggcatagccc tgctccaaaa agagccggcg cgcctgctc
//
LOCUS       DASUBI010000358         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_667932, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000358 DASUBI010000000
VERSION     DASUBI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667932"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFU72_06905"
     CDS             <1..767
                     /locus_tag="VFU72_06905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5423250.1"
                     /translation="PARDGLVIQGWLLRPPGAAEGARLPLIANVHGGPTARWGPTFHG
                     TWHDWAQSLAGAGYAVLLPNPRGSTGRGAAFTSANRSDLGGKDFEDVLAGVDYLIERG
                     IADPERLGIGGWSYGGFLTAWAVGQTDRFKAAVAGAAVTNWPSKVGTTDIRTGNEKNF
                     PGPLHEQPDAFWERSPVRYLGRISTPTLVLHGEADPRVPPGQGYELYQGLREMGVPTD
                     FITYPRQKHAFHEKAFQQDLLQRVVGWFQRWIPVEQ"
     gene            788..970
                     /locus_tag="VFU72_06910"
     CDS             788..970
                     /locus_tag="VFU72_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423251.1"
                     /translation="MIARNIAPHLPGSGIIEGREPLLGQPAPEWQLRDEAGNLVTLKR
                     LTRQQPLLLHLYRGAW"
     gene            989..1603
                     /locus_tag="VFU72_06915"
     CDS             989..1603
                     /locus_tag="VFU72_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5423252.1"
                     /translation="MSQLRQHYWELRGLGVELVTAANDTPETNAALREQLDLPFTLLS
                     DPEAEVARAYGAFHENEHRGRKIARVAMFLIGSAAQEQRILWEYVGPTSRHRPAPSRL
                     SEEVQRALGRRELLVSVVVPSTWQLEQSIAALHQPPLGYARTPAPPPAHVVLLQRDYL
                     RELAMQSEAEVQRLTEEGWTLAAVSPEYAGAQATGQRYVFRRRA"
     gene            complement(1760..1945)
                     /locus_tag="VFU72_06920"
     CDS             complement(1760..1945)
                     /locus_tag="VFU72_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423253.1"
                     /translation="MSAKLLLAALAILAGYVLLGLLWLAVEGATSLGALLFFALGLPV
                     AAAALWWSTRGGPPRVR"
     gene            complement(1952..3307)
                     /gene="mgtE"
                     /locus_tag="VFU72_06925"
     CDS             complement(1952..3307)
                     /gene="mgtE"
                     /locus_tag="VFU72_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU5423254.1"
                     /translation="MTAERERAPSLEAGLERGDTAALTAALRALTEQGDLPERLARLT
                     PAEVRRLAELLGDDALGELLGEIDPYDAAALLSRLAVVDAADVLEAMEPDDAADVVGE
                     FAPEEADRILVAMEPIEAAELRDLLSYPPDSAGGRMTPAYVAVAPDLSARQAIAALQK
                     LAEAAETVAYVYVTDAQEHLLGVLSLRRLVLSPPDTPIARIIDRDVVSVPVTADQEQA
                     ARLLLAEDFLALPVVDADNRLLGIITADDVADIIEQETTEDIERLGGSEPLEEPYLRA
                     APQRLWRKRVGWLLALFFAEAYTGTVLRHYQNEVETVVALSFFIPLLIGTGGNVGSQV
                     VTTIVRAMAVGDVRFGDMFRIVRKEFVTGIMLGVVMAVATFIRAMTLRVGVDVGLTVA
                     MAVTAIVIWAAIVGAILPLVLHKLRVDPAVVSAPFISTLVDGTGLVIYFNVARLFLRL
                     H"
BASE COUNT          529 a         1246 c         1250 g          469 t
ORIGIN      
        1 ggcctgcgcg ggacggcctc gtgatccagg gctggctgct gcgcccgccg ggcgcggcgg
       61 agggcgcgcg cttgccgctg atcgccaacg tgcacggcgg gccgacggcg cgctggggtc
      121 cgaccttcca cggcacctgg cacgactggg ctcagagcct ggcaggggcc ggctacgccg
      181 tgctgctgcc caacccgcgc ggcagcaccg ggcgcggcgc cgccttcacc agcgccaacc
      241 ggagcgatct cggcgggaaa gacttcgagg acgtgctggc cggggtcgat tatttgatcg
      301 agcgggggat tgccgacccg gagcggctgg gcatcggcgg ctggagctac ggcggcttcc
      361 tgaccgcctg ggccgtcggc caaaccgacc gcttcaaggc ggccgtggcc ggcgcggccg
      421 tgaccaactg gcccagcaaa gttggcacga ccgatatccg caccggcaac gagaagaact
      481 tccccggccc gctacacgag cagcccgacg ctttctggga gcgctcgccg gtgcgctacc
      541 tgggccggat cagcacaccg acgctggtgc tgcacggcga ggccgacccg cgcgtgccgc
      601 ccggccaggg ctacgagctc taccagggtc tgcgcgagat gggcgtgccg accgacttca
      661 tcacctaccc gcgccagaag cacgccttcc acgagaaggc tttccagcaa gacctcttgc
      721 agcgcgttgt cggctggttc cagcgctgga ttccggtcga acaataagga gtaaggagca
      781 cgccgggatg atcgcgcgca acatcgcgcc gcacctgccg gggagcggca tcatcgaggg
      841 acgcgagccg ctcttgggcc agccggcgcc ggagtggcaa ctgcgcgacg aggcgggcaa
      901 tctcgtcacg ctgaagcggc tgactcgcca gcagccgtta ctgctccatc tctaccgcgg
      961 cgcctggtga cccacctgac gacgctatct gtcgcagttg cggcagcact actgggagct
     1021 tcgtgggctg ggtgtcgagc tagtgaccgc ggccaacgat acgccggaga ccaacgcggc
     1081 gctgcgggag cagctcgatc tgccgttcac gctcctctcc gatcctgagg cggaggtcgc
     1141 ccgcgcctac ggcgccttcc acgagaacga gcatcgtggc cgcaagatcg cgcgcgtggc
     1201 catgttcctg atcggctcgg ccgcgcagga gcagcgcatc ctctgggagt acgtcgggcc
     1261 gacctcgcgc caccgccccg cgccgtcgcg cctctccgag gaggtgcagc gcgcgctcgg
     1321 gcgccgcgag ctgctcgtct cggtcgtcgt cccctccacc tggcagctcg agcagtccat
     1381 cgcggcgctg caccagccgc cgctcggcta cgcgcgcacg ccggcgccac cgcccgcgca
     1441 cgtcgtcctg ctgcagcgcg actacctgcg cgagctggcg atgcagagcg aggccgaggt
     1501 gcagcggctg accgaggagg gctggacgct ggccgccgtc agccccgaat acgccggcgc
     1561 ccaggcgacc ggccagcgct acgtcttccg ccgccgggcg tgatgcggtt tccccgtcgc
     1621 cagtgcgttg ggccacgagg gcagacacag gggtctgccc ctacaggatc ggcgccgcgt
     1681 ttttccggta ggggcggccc cccgtggccg ccctctcccc cctacaaccc atcttaaccg
     1741 taggccgccc caccaacccc taccgcaccc gcggcgggcc gccgcgggtg ctccaccaga
     1801 gggcggccgc ggccaccggc aggccgaggg cgaagaagag gagcgcgccg aggctggtcg
     1861 cgccctcgac ggccagccag agcagaccga gcagcacgta gccggccagg atcgccagcg
     1921 cggccagcag cagcttggcg ctcatcgcgg ctcagtgcag ccgcaggaac agcctggcga
     1981 cgttgaagta gatcaccagc ccagtcccat cgaccagggt cgagatgaag ggggccgaga
     2041 cgaccgccgg atcgacgcgc agcttgtgca gcaccagcgg caggatcgcg ccgacgatgg
     2101 ccgcccagat cacgatggcg gtgaccgcca tcgccaccgt cagcccgacg tcgaccccca
     2161 cccgcagcgt catggcccgg atgaaggtcg cgacggccat gaccaccccg agcatgatgc
     2221 cggtgacgaa ctccttgcgc acgatgcgaa acatgtcgcc gaagcgcacg tcgccgaccg
     2281 ccatggcgcg cacgatggtg gtcacgacct gcgagccgac gttgccgccg gtgccgatca
     2341 ggagcgggat gaagaaggag agggccacca ccgtctcgac ctcgttctgg tagtggcgca
     2401 gcacggtgcc ggtgtaggcc tcggcgaaga agagggccag cagccagccg acgcgcttgc
     2461 gccacagccg ctgcggcgcc gcgcgcaggt acggctcctc cagcggctcg gagccgccca
     2521 gccgctcgat gtcctcggtc gtctcctgct cgatgatatc ggccacgtcg tcggcggtga
     2581 tgatccccag caggcggttg tcggcgtcga ccaccggcag cgccaggaag tcttcggcca
     2641 ggagcagccg cgcggcctgc tcctggtcgg ccgtcaccgg cacgctgacg acatcgcggt
     2701 cgatgatgcg cgcgatcggg gtatcgggcg ggctgaggac caggcggcgc agcgagagga
     2761 cgcccagcag atgctcctgg gcgtcggtga cgtagacgta ggcgacggtc tcggcggcct
     2821 cggccagctt ctgcagcgcg gcgatcgcct ggcgcgcgct caggtcgggc gcgactgcca
     2881 cgtaggcggg ggtcatgcgg ccgccggcgc tgtcgggcgg gtagctgagc aggtcgcgca
     2941 gctcggcggc ctcgatcggc tccatcgcca ccaggatgcg gtcggcctct tcgggcgcga
     3001 actcgccgac cacgtcggcg gcgtcgtccg gctccatggc ctccagcaca tcggccgcgt
     3061 cgaccacggc caggcggctg aggagggcgg cggcgtcgta gggatcgatc tcgcccagca
     3121 gctcgcccag ggcgtcgtcg cccagcagct cggccaggcg gcgcacctcg gcgggcgtga
     3181 ggcgggccag gcgctcgggc agatcgccct gctcggtcag cgcgcgcagg gccgccgtca
     3241 gcgcggcggt gtcgccgcgc tccagccctg cctctaacga tggtgcgcgc tcccgctccg
     3301 ccgtcacggc gctatggtac gcgtacgagc cggatagggc gagcggtgtc cttcgagggg
     3361 catgggatgt aaggcaagac ggatccttcg actgcgctca ggatttggcg tttggacaaa
     3421 gcagataccg tccgggatac agtgcaaccg gactggcggg acgggtggcg gcgtgggcgg
     3481 ggcctcaggc ggct
//
LOCUS       DASUBI010000359         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_963205, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000359 DASUBI010000000
VERSION     DASUBI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963205"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VFU72_06930"
     CDS             complement(<1..1829)
                     /locus_tag="VFU72_06930"
                     /inference="COORDINATES: protein motif:HMM:NF033179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA-like heteromeric transposase endonuclease
                     subunit"
                     /protein_id="HEU5423255.1"
                     /translation="MAAQQAWSLAQPSGFDLGFVTADGTRHRGRLTECWAQPFEQGQP
                     ARKFPSYKGQKNFTGWRWVATTGRHVGFESWLERDHLTLLDYDPALTAICSQPFTVFW
                     REGRRERMHTPDYFARAVDGSALVLDVKSQAQITQRDAVAFAAIARVCGEVGWRYERV
                     GELDPVYRANMRWLAAYRHPRFGESPLLQRVRACFAQPTPLFAGAQAAGDRITILPLV
                     YHLLWRQMLRADLRAQRLNPMTLVWDAHAVDGGGSRSPATREQQPEAGAMPALRVPDL
                     SDRPQPEGALMTDAARVLRVEDHVRFEGRTWRVAALAGAAVTLADHEGRRKQLWLTEL
                     YLHGSPVHPDKRPRHVPSGTLKLLKEPVLKQARWWETHILEVITGVPPGAPPDTAPRP
                     EYDPSTRTLAEREQAKSAELRRAGHGRGASAASVKVKRLRYQARGLEGLVDGHSDPDR
                     ERAVDPRYVAALRQLIKEAKDESTRNAKFFRWKTELRLTELYGRGAVPLPSRATFYRL
                     FDELTVGRHTTGSARTRRSVAVQPPAPFGVYSVTRPGELMEIDSTPLDIAVRLPRGVV
                     GRVELTAIIDVGSRSVPAAVLRPTTKAVDASLLVARTVTPELMR"
     gene            complement(2162..2416)
                     /locus_tag="VFU72_06935"
     CDS             complement(2162..2416)
                     /locus_tag="VFU72_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5423256.1"
                     /translation="MSSSSPPPDPRLHAFGLNIRRLRERAGLSLEELAERADLSFRGL
                     VYIEHGRRNAGVLTVFKIADGLRVPATALFDYPPPPADSR"
BASE COUNT          426 a          932 c          988 g          405 t
ORIGIN      
        1 cgcatcagtt ccggggtcac cgtgcgcgcc accagcagcg aggcgtccac ggccttggtg
       61 gtcggacgca gcaccgcggc gggcaccgac ctgctgccga cgtcgatgat cgcggtcagc
      121 tccacccggc ctaccactcc ccgaggcagg cgcacggcaa tgtccagggg cgtggagtcg
      181 atctccatca gctcgccggg gcgcgtaacg ctgtacacgc cgaacggggc gggcggctgg
      241 acggcgaccg agcgacgggt acgggcagag ccggtggtgt gccggcctac ggtcagctcg
      301 tcgaagagcc ggtagaacgt ggcacgcgag ggcagcggca cggcgccgcg gccgtacaac
      361 tcggtcaggc gcagttcggt cttccagcgg aagaacttgg cgttgcgcgt ggactcgtcc
      421 ttcgcctcct tgatcaattg gcgcagcgcg gccacgtacc gggggtccac ggcgcgttcg
      481 cggtccgggt cgctgtgccc gtcgaccaag ccctcgagcc ctcgcgcctg gtatctcagc
      541 cgcttgacct tgacgctcgc cgcgctggcc ccgcggccgt ggcccgcgcg gcgcagctcg
      601 gcggatttgg cctgctcccg ttcggccagc gtgcgcgtgc tcgggtcgta ttccgggcgc
      661 ggcgcggtgt ccggcggcgc accggggggc acgccggtga tgacctccag gatgtgggtc
      721 tcccaccagc gagcctgctt gagcaccggc tccttgagca gtttgagggt tccggagggc
      781 acatggcgcg gccgcttgtc ggggtgtacc ggcgagccgt gcaggtagag ctccgtgagc
      841 cacagttgct tgcgccggcc ctcgtggtcg gcgagcgtga ctgcggcgcc cgcgagcgct
      901 gccaccctcc aggtgcgacc ctcgaagcgg acgtggtcct ccacgcgcag cacgcgcgcg
      961 gcgtcggtca tcaacgctcc ctcgggttgc ggtcgatcgc tcaggtccgg cacccgcagc
     1021 gcgggcatcg cgcccgcctc gggctgctgc tcgcgcgtgg ccggcgacct gctaccccca
     1081 ccgtccacgg cgtgcgcgtc ccacaccagg gtcatcgggt tcagacgctg cgcgcgcagg
     1141 tcggccctga gcatctgccg ccaaagcagg tggtagacca gcggcaggat cgtgatccgg
     1201 tcgcctgcgg cctgggcgcc agcgaacagc ggcgtgggct gcgcgaaaca ggcgcgaacg
     1261 cgctgcaata gcggcgattc cccgaaccgg ggatgacggt aggccgccag ccagcgcatg
     1321 ttcgcgcggt agaccggatc aagctcaccg acccgctcat agcgccagcc cacctcgccg
     1381 cagacccggg caatggcggc gaacgccacg gcgtcgcgtt gcgtgatctg cgcctgcgac
     1441 ttgacgtcga ggaccagcgc cgacccgtcc acggcccggg cgaagtagtc cggagtgtgc
     1501 atgcgttctc gccggccctc gcgccaaaag acggtgaacg gctgggagca gatagccgtc
     1561 agcgccgggt cgtagtcgag cagcgtcaga tggtcgcgtt ccaaccagga ctcgaaaccg
     1621 acgtgacgac ccgtcgtggc cacccagcgc cacccggtga aattcttctg ccctttgtag
     1681 gaagggaact tacgcgccgg ttggccttgt tcgaagggct gcgcccagca ctcggtcagg
     1741 cgtcctctgt gccgtgtgcc gtccgcggtc acgaagccga gatcgaaccc ggaaggctgg
     1801 gccaacgacc aggcctgttg cgccgccacg caccctccgc agctcactga tatgaggtcg
     1861 cgtatgagag tactgcaaaa gcctcacgga tgggatcacg atccgggatt cgcgcgtcgg
     1921 cacctcgaat cgagaggtga cggcgcccgc cgcatcctca gagcccttct ctgccgacgc
     1981 tctgacgagg gaagccacga tgtcggtgag ctggaagcgc gcggccacgc ggcccgcggt
     2041 gacttgcggc acctgcgccg gcggcacggc accccggcgg cgacgggatc acggaacgcc
     2101 ggcgatgtgc ctgaccgtga ggggccgcta gggctcgtcc cggggcttgg ggctaaaaac
     2161 cctatcttga gtcggcgggt ggcggcggat agtcgaagag cgccgtcgcg ggcacccgca
     2221 gaccatccgc gatcttgaag acggtcagca ccccggcgtt gcggcggccg tgctcgatgt
     2281 agaccaggcc gcggaagctc aagtctgccc gctctgccag ttcctcgagc gagagcccgg
     2341 cgcgctcgcg cagtcggcgg atgttcaggc cgaaggcgtg caggcggggg tccgggggcg
     2401 ggctgctgga agacacgctg cgacaccagc agtccaagat cagctaaacc atgaacagtt
     2461 gtgcataatc gtgatcaatg ctggcgggac gggcgacaaa gacccgggcg ggtggggcgg
     2521 cgccgagcac ggacatcgag gaggacatca gtgagcggga cgagcgacgc gatcaacgct
     2581 tacaagcgcc attacgggta cgcgctgcgc gacctggacc ggttgatgcg cttcatggac
     2641 ctgggctggg tgcgccacta gccgccggcg gtcgccctgg tccttgaggg catcgacgac
     2701 aggccccgtg ccgtggtcga cgagctggtc gaggcggagc gcggcgcgcc g
//
LOCUS       DASUBI010000360         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_711791, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000360 DASUBI010000000
VERSION     DASUBI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711791"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFU72_06940"
     CDS             complement(<1..822)
                     /locus_tag="VFU72_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5423257.1"
                     /translation="MGGRGSEHGAAPLRLVIAGGGTGGHVQPAVATLQELRRRVPLQP
                     LWLGSAAGLERSVAADEGIPFRAIAVGKLRRYFSLQTPLDALRIPLGVAQSLRHLRAA
                     RPDLVFSTGGFVSVPTVVAARLLGIPSLTHEQTAVIGLATRINARFVDVIALSYERSR
                     ALLGANRGQVVVTGDPIRSSLFDGDPAGAFQAFGLDPNLPLVYVTGGALGAQAINSAV
                     REALPRLLARVQVIHQCGRTAGEEERLHVAREALPHELQLRYAIRPFIGPELADIY"
     gene            1012..1368
                     /locus_tag="VFU72_06945"
     CDS             1012..1368
                     /locus_tag="VFU72_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5423258.1"
                     /translation="MDVFRGRLTQAFQETAGEIADIDDELQGLALDDTAAEGVDNHIG
                     DDSDQLFERERLMTIRGELVDRRLAIQQALQKLDDGTYGICERCGKPIAPERLEALPF
                     ARYCLDCQDIIDRQGR"
     gene            1374..1922
                     /gene="lspA"
                     /locus_tag="VFU72_06950"
     CDS             1374..1922
                     /gene="lspA"
                     /locus_tag="VFU72_06950"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5423259.1"
                     /translation="MSDEPRRHQTRLPALLAVAIAGLVILADQLSKLAVLDVLGPHGD
                     RTVIPIVPGLLRLIYVQNTGAAFGMFQGRSPVILVLAVAVIILLLYYFREAIAASRWL
                     ALALGLQIGGAIGNMIDRLNHGFVVDFINVPHWPTFNVADSAVTVGVIMLAIYLIGRD
                     APRAAEDTPSARPVQPEAQERP"
     gene            1969..2901
                     /locus_tag="VFU72_06955"
     CDS             1969..2901
                     /locus_tag="VFU72_06955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5423260.1"
                     /translation="MTEAVRVALDVTGEEDGERLDRLIAGRVADISRSYAQNLVKQGD
                     VLVNGAAAKPAARVRAGDRVEVALPPVGQPEELTPYHFPVPVIYEDDDVIVFDKPAGL
                     VVHPAPGHEHGTLANIIRSLRPDLELVKSQRPGIVHRLDKDTSGLIVVAKNEAARLYL
                     LKQWQNRDVIKRYTTLVNGVVAEDEGTIDAPIGRDPNNRKRMWVVAKGRPAVTHFRAM
                     RRFADATLLDVQIVTGRTHQIRVHMAFIGHPVIGDQTYGKRPFRYPVERQFLHASYLR
                     FFLPGGHPIALETPLPPDLQAVLTALESERARGS"
     gene            2898..4202
                     /locus_tag="VFU72_06960"
     CDS             2898..4202
                     /locus_tag="VFU72_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEU5423261.1"
                     /translation="MSEDAARAAVEAIFRAALHAVDPERLVREALGRQREDIVVAGRR
                     FAVPGRVLLLAVGKAALPMARGAEEALGDRIAGGLAVTKRGFLAGQVPPARIAVLEGA
                     HPVPDAASLAAGERLLELADALGPDDLALVLISGGGSALVEAPSAGVTLAEIASTTDL
                     LLRHGADITTLNTVRRRLSRLKAGGLARAAAPARVINLIVSDVLGSPLPIIASGLSVD
                     PGALAPSVLAAVRASDAWRELPPTVRDALSAPLHDERPDNVVWSEVLADAARAARAAT
                     EAARERGYQAMLLATRFEGEAPDFGGFWTQLALGVARGEFALARPACLVGAGEMTVTV
                     RGAGRGGRNTEMALAAALMLSGQRGVAVASLATDGDDGSSGAAGGVVTGASVAAAEAA
                     GLDPRRLLEANDSARFLAACGGLIVTGATGTNVNDLYLALIE"
BASE COUNT          624 a         1463 c         1544 g          592 t
ORIGIN      
        1 gtagatgtct gccagctccg gcccgatgaa gggccggatc gcgtagcgca gctggagctc
       61 gtgcggtagc gcctcgcgag cgacgtgcag ccgctcctcc tcgcctgcgg tacggccgca
      121 ctggtggata acctggaccc gcgccagaag tcgcggcaac gcctcgcgca cggcgctgtt
      181 gatcgcctgc gcgccgagcg cgcctccggt cacgtagacc agcggcaggt tcggatccag
      241 gccgaaggcc tgaaaggcgc cagccgggtc accgtcgaag agcgaggagc ggatcgggtc
      301 gccggtgacg acgacctggc cgcggttcgc gccgaggagg gcacggctac gctcgtacga
      361 gagcgcgatt acatcgacga agcgcgcatt gatgcgggtc gccagcccaa tcaccgccgt
      421 ctgctcgtgc gttaggctgg gaatgcccaa caggcgcgcc gcgaccacgg tcgggacgct
      481 gacgaagccg ccggtgctga agaccagatc gggccgggcg gcgcgcagat gccgcagcga
      541 ctgcgcgaca ccgagcggga tgcgcagcgc gtcgagcgga gtctggagcg agaagtagcg
      601 gcgcagctta ccgaccgcga tggcgcggaa gggaattccc tcgtccgccg ccacgctgcg
      661 ttcgagacca gcggcgctgc cgagccagag cggctggagc ggcacacgcc ggcgtagctc
      721 ctgtagcgta gcgaccgccg gctggacgtg cccgccggtg ccgcccccgg cgatgaccag
      781 ccgcagcggc gccgcgccgt gctccgaacc ccgccctccc acgatccacc ttccccgctg
      841 gcaccgaatc tgccccgcgc aatcctatgt cgcggcaacc tcgggcggca aagcagcggc
      901 gatcgccggt cgcgccgtct ggtgcctctg ctacaattct gggaccactg gctggatttc
      961 cagccggtgg ctgaacgagg ggcgcggaca actgtggtat ctcaggaact gatggacgtc
     1021 ttccggggcc gtttgacaca agcgtttcag gagacggcgg gcgagatcgc cgacatcgac
     1081 gacgagctgc aagggctcgc gctcgacgac accgccgccg aaggcgtcga caaccacatc
     1141 ggcgacgact cggatcagct cttcgagcgc gagcgcctga tgacgatccg gggcgagctg
     1201 gtcgaccgcc ggctggcgat ccagcaggcc ctgcagaagc tcgacgacgg cacctacggc
     1261 atctgcgagc gctgcggcaa gccgatcgcg ccggagcgcc tggaagccct cccgttcgcg
     1321 cgctactgcc tcgactgcca ggacatcatc gaccgtcaag gacgatagcc acggtgagtg
     1381 acgaaccacg ccggcatcag acgcgcttgc ccgccttgct ggcggtcgcg atcgccggtc
     1441 tggtgatcct ggccgaccag ctcagcaaac tcgccgtgct cgacgtgctc ggcccgcacg
     1501 gcgaccgcac cgtgatcccg atcgtgccgg ggctgctgcg cctgatctac gtgcagaaca
     1561 ccggcgcggc cttcggcatg ttccaggggc gcagcccggt catcctggta ctggcggtgg
     1621 cggtcatcat cttgctgctc tactactttc gcgaagcgat cgcggccagc cgctggctcg
     1681 cgctggcgct cggcctacag atcggcggcg ccatcgggaa catgatcgac cggctgaatc
     1741 atggcttcgt ggtcgacttc atcaatgtgc cgcactggcc caccttcaac gtggccgaca
     1801 gcgccgtcac ggttggggtg atcatgctcg cgatctacct catcggtcgc gacgcgccgc
     1861 gcgcggccga ggacacgccc agcgcccgcc ccgtccagcc ggaggcccag gagcggccct
     1921 agcccggcgg tcggcgcccg cgggcggcac gcagcatgag gggccctgat gaccgaagcg
     1981 gtgcgcgtcg ccctcgacgt gacgggcgag gaagacggtg agcggctcga ccggctgatc
     2041 gccgggcggg tggccgacat cagccgcagc tacgcgcaga atctggtcaa acaaggtgac
     2101 gtgctggtca acggcgcggc cgccaagccc gccgcgcgcg tgcgcgcggg cgaccgggtc
     2161 gaggtggctc tgccgccggt cgggcagccc gaagagttga cgccgtacca ctttccggtg
     2221 ccggtgatct atgaggacga cgacgtgatc gtctttgaca agccggccgg gctggtcgtg
     2281 caccccgcgc cggggcacga gcacggcacg ctggccaaca tcatccgctc actccggccg
     2341 gatctagagc tggtcaagtc acagcgcccc ggcatcgtgc accggctcga caaagacacc
     2401 tcgggtctga tcgtggtggc caagaacgag gccgcgcgcc tctacctcct gaagcagtgg
     2461 cagaaccgcg acgtgatcaa gcgctacacc acgctggtca atggcgtggt cgcagaggat
     2521 gaggggacca tcgacgcgcc gatcgggcgc gatcccaaca accgcaagcg catgtgggtg
     2581 gtggccaagg ggcgaccggc ggtgacccat ttccgggcca tgcggcgctt cgccgacgcc
     2641 acgctgctcg acgtgcagat cgtcaccggc cgcacgcatc agatccgcgt ccacatggct
     2701 ttcatcgggc acccggtgat tggcgaccag acctacggca agcgtccctt ccgctacccg
     2761 gtcgagcggc agttcctgca cgccagctac ctgcgcttct tcctgcccgg cggccacccg
     2821 atcgcgctgg agactcccct gccgcccgat ttgcaggctg tgctgacggc gctagagtcc
     2881 gagcgggcac gcggctcgtg agcgaagatg cggcgcgcgc ggccgtcgaa gccatcttcc
     2941 gtgcggcgct gcatgcggtc gatcccgagc ggctggtgcg cgaggcgctc ggccgccagc
     3001 gcgaggacat cgtcgtcgcg gggcggcgct tcgcggtgcc ggggagggtg ctgctgctgg
     3061 cggtgggcaa ggcggcgctt ccgatggcgc gcggcgccga ggaggcgctg ggcgaccgca
     3121 tcgccggggg cctggccgtg accaagcgcg gctttctggc aggtcaggtg cccccagctc
     3181 gcatcgccgt gctggagggc gcgcacccgg tgcctgatgc ggcgagcctg gccgccgggg
     3241 agcgactgct ggagctggca gacgcgctcg gccccgacga cctggcactg gtgctgatct
     3301 cgggcggcgg ctcggcgctg gtcgaggcac ccagcgccgg ggtgaccctg gccgagatcg
     3361 cctcgacgac ggatctcctg ctgcgccacg gggcggacat caccacgctc aacacggtgc
     3421 ggcgccggct cagccggttg aaggcgggcg gcctggctcg cgccgccgcc ccggcgcgcg
     3481 tgatcaacct gatcgtctcc gacgtgctcg gcagcccgct gccaatcatc gccagcggcc
     3541 tctcggtcga tcctggcgcg cttgctccgt ccgtcctggc cgccgtgcgc gcgtcggacg
     3601 cctggcgcga gttgccgccg acggtgcggg acgcactcag cgcgccgctg cacgacgagc
     3661 ggcccgacaa cgtggtgtgg agcgaggtgt tggccgacgc ggcccgtgcg gcgcgggcgg
     3721 cgacggaggc ggcgcgcgag cgtggttacc aggccatgct cctcgccacg cgcttcgaag
     3781 gagaggcgcc cgacttcggc ggcttctgga cgcagctcgc gctcggcgtg gcccgcggcg
     3841 agttcgcgct ggcgcgtccg gcctgcctgg tcggtgcggg cgagatgacc gtgacggtgc
     3901 gcggggcggg acggggcggc cgcaacaccg agatggcgct ggcggcggcg ctgatgctct
     3961 caggccagcg gggtgtggcg gtggccagcc tggctacgga cggggacgac ggcagcagcg
     4021 gcgcggcggg cggggtggtg accggcgcga gcgtggcggc ggccgaggcg gcgggtctcg
     4081 atccccggcg gctgttggag gcgaacgaca gcgcgcgctt cctggcggcc tgcggcgggc
     4141 tgatcgtgac cggcgcaacg ggcacgaacg tcaacgacct gtatttggcg ctcatcgagt
     4201 gaggggccgg cggcggccaa gcg
//
LOCUS       DASUBI010000361         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1043483, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000361 DASUBI010000000
VERSION     DASUBI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043483"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..409)
                     /locus_tag="VFU72_06965"
     CDS             complement(104..409)
                     /locus_tag="VFU72_06965"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5423262.1"
                     /translation="MDERDKPSTLEELRARREEILSIAAARGASNVRVFGSVARDDAG
                     PESDIDFLVDLERGRDLFDLGGRISELEDLPGRPVDVVEIGKPSRVAGRIQREAVQL"
     gene            complement(514..3741)
                     /locus_tag="VFU72_06970"
     CDS             complement(514..3741)
                     /locus_tag="VFU72_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5423263.1"
                     /translation="MQEGTSNGQAAERWPGLRYRLVGPFRGGRVIAVAGHPWEQATFY
                     FGSTGGGVWKSNDTGMTWRNISDGYFKRASVGALAIAESDPNVLYAGMGEATIRGNVS
                     HGDGVYKTTDGGKSWRNVGLVETRNISKVRVHPDDADLVYVAALGHAHGPNKERGIFR
                     STDGGEHWEQVLFRSEETGAIDLTLDATNPRNLYAAFWQARRYPHTLVGGGPESGLFR
                     SRDGGDSWEDISHNPGLPEGVFGKIGIVASPAQGGRVWALVEAEKGGVYRSDDGGDTW
                     TLVSDDRNLLQRPWYFTHIFADPEDAETVWVLNFYTYKSTDGGKTFTQIPTPHADNHD
                     LWIDPNNTRRMIIGHDGGATVSLNGAETWSTVLNQGTVEFYHVTTDNSVPYRLYGAQQ
                     DNTTISVPSRSNTSAITVQDWFEVGGGESGYIAVRPDNPDIIFAGSYGGMLTRYDRST
                     GQRKNINVWPEGGTGYGAGELKYRFQWTYPIVLSPHDPNILYVTSQYVHRSTDDGQSW
                     QTISPDLTRAAPHTLGRSGGPITFDNTGAEYYATIFAFAESPVQAGVFWAGSDDGLVH
                     VSRDHGQSWQNVTPPDLPEFALISIIDAAPHDPGTAYLAATRYKLDDFTPYLYRTSDY
                     GQSWQKITQGIPAEDFTRVIRADPNHQGLLYAGTETGVYVSFDDGQHWQPFQQNLPVV
                     PIHDLEIKDTDLIAATHGRGFWSIDDLTPLYQMQGGQAPLQLFQPRPTVQFKTNFGFG
                     GGAGPGNHFQMTGATMYTYRLEKNEQGGEERVNLDAGQNPPEGVLFRYSFAQAPEGEV
                     KLTISDGQGNEIRGFTSKKPEGASEVKPMVAGESFEQMMEQAGVTEPEDKQEPKVPTA
                     AGMNRFVWDMRYPGAVEVPGDKSMQFVGGSRGPSAPPGTYTVTLSANGQSQSQSFTLL
                     KDPRNPATDEDLQAQFQFLMQVRDKLSQTNEAINRIRTVREQVVGWGKRLAGKDSGAA
                     VREVGKPLVRQLSEIEEQLIQVHAKSRQDTLNYPVMLNAKLAGLMRSAASAYARPTEQ
                     SYAVFDDLSKQVDAQLERLNNLLQSELPKFNQAVQQAQVPAVEAP"
     gene            complement(3973..>4536)
                     /locus_tag="VFU72_06975"
     CDS             complement(3973..>4536)
                     /locus_tag="VFU72_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5423264.1"
                     /translation="ADLVILSLHHHEEGASTEDPAAFARDFARQMIEAGADLVVGHGS
                     HLLRGLELYQGKPIFYSLGNFIAQNDLTYKLPADDYEQFRVDPNLTPGEVYRQRSEDG
                     AKGFPADSRYWETLVPLLRFEGGALKEIALYPVTLGHGQPTSRRGRPRLAEGEQAGVI
                     LERFAALSRPFGTEIEVGSDRAEVRLS"
BASE COUNT          645 a         1487 c         1580 g          824 t
ORIGIN      
        1 tcgtgcccag cctgtagggg cggacccccg tgtccgccct cgtgcggcaa ggacgatgcg
       61 tcgggtaaac ggcgcaatac gggctgatga ccaaaccgtt ccattacagc tgcacagcct
      121 ctcgctggat gcggccggcc acgcgtgagg gcttcccaat ctcgacgaca tccaccggcc
      181 ggcccgggag gtcttcgagt tcggagattc gaccacccaa atcgaagaga tcgcgcccgc
      241 gctccaggtc caccaggaag tcgatgtcgc tctccggacc ggcatcgtcg cgcgccaccg
      301 agccgaagac gcgcacgttg ctggcaccgc gagcggccgc gatgctcagg atctcctcgc
      361 gccgcgctcg cagctcttcc agtgtcgacg gcttgtcgcg ctcgtccatg gtctgccact
      421 ccggcgctgt tccgacggtg tattcaaagc gggaccgggc gtccatcccg agtcagctac
      481 ggacgccacg atcccgcgtg tccattctac ggcctatggc gcctcgaccg ctggcacctg
      541 cgcctgctgc accgcctggt tgaacttcgg cagctcgctt tgcagcaggt tgttcagccg
      601 ctcgagctgc gcgtcgacct gcttcgacag atcgtcgaag acggcgtagg actgctcggt
      661 cgggcgcgcg taggcgctgg cggccgagcg catcaggccc gccagcttgg cgttgagcat
      721 caccgggtag ttgagcgtgt cctgccggct cttggcgtgg acctggatca attgctcctc
      781 gatctcgctg agctgccgca ccagcggctt gcccacctcg cggaccgctg cgccgctgtc
      841 cttgccggcc aggcgcttgc cccagccgac gacctgctcg cgcaccgtgc ggatgcggtt
      901 gatcgcctcg ttggtctggc tcagcttgtc ccgcacctgc atcaggaact ggaactgcgc
      961 ttggaggtcc tcgtccgtgg ccgggttgcg cgggtccttg agcagcgtga agctctgact
     1021 ctgcgactgg ccattggcgc tcagcgtgac cgtgtacgtg ccgggaggcg cgctcgggcc
     1081 gcgcgagccg ccgacgaact gcatcgattt gtcgccgggc acctcgaccg cgccggggta
     1141 gcgcatgtcc cagacgaagc ggttcatgcc cgccgccgtg ggcaccttcg gctcctgctt
     1201 gtcctcgggc tcggtgacgc ccgcctgctc catcatctgc tcgaaggact cgccggccac
     1261 catcggcttg acctccgacg cgccctccgg cttcttgctg gtgaagccgc ggatctcgtt
     1321 gccctgccca tcgctgatgg tcagcttgac ctcgccctcg ggcgcctgcg cgaagctgta
     1381 gcggaagagc accccctcgg gcgggttctg cccggcgtcg aggttgaccc gttcctctcc
     1441 accctgctcg ttcttctcca ggcggtaggt gtacatggtg gcgccagtca tctgaaagtg
     1501 attgcctggg ccggcgccgc cgccgaagcc gaagttggtc ttgaactgca ccgtcggccg
     1561 cggctggaaa agctgcagcg gcgcctgacc gccctgcatc tgatacaggg gagtcagatc
     1621 gtcgatcgac cagaagccgc gtccatgcgt ggcggcgatc agatcggtgt ccttgatctc
     1681 caggtcgtgg atcggtacga ccggcaggtt ctgctggaag ggttgccagt gctgcccgtc
     1741 gtcgaaggag acgtagacgc ccgtctccgt gccggcgtag agcagcccct ggtggttggg
     1801 atccgcccgg atgacccgcg tgaagtcctc ggccgggatg ccctgggtga tcttctgcca
     1861 cgactgcccg tagtcgctgg tcctgtagag gtagggggtg aagtcgtcca gcttgtagcg
     1921 ggtggcggcc aggtaggccg tgccggggtc gtggggcgcg gcgtcgatga tcgagatcag
     1981 ggcgaactcg ggcaggtcgg gtggggtgac gttctgccag gactggccgt ggtcgcgcga
     2041 gacatgcacc agaccgtcgt cggagccggc ccagaagacg ccggcttgga cgggggactc
     2101 ggcgaaggcg aagatggtgg cgtagtactc cgctccggtg ttgtcgaagg tgatcggccc
     2161 gccggagcgg cccagcgtgt gcggcgcggc gcgcgtcagg tcgggcgaaa tcgtctgcca
     2221 gctctggccg tcgtcggtcg aacgatggac gtactgcgag gtgacgtaga ggatgttcgg
     2281 gtcgtgcggc gagagcacga tcgggtaggt ccactggaag cggtacttga gctcgcccgc
     2341 gccgtagccg gtgccgccct ccggccagac gttgatgttc ttgcgctggc cggtgctccg
     2401 gtcatagcgc gtgagcatgc cgccatagct gccggcgaag atgatgtccg ggttgtcggg
     2461 gcgcacggcg atgtagccgc tctcgccgcc gccgacctcg aaccagtcct gcacggtgat
     2521 ggccgaggta ttggagcggc tgggcacgct gatggtggtg ttatcctgct gcgcgccgta
     2581 gaggcgatag ggcaccgagt tgtcggtggt gacgtggtag aactcgaccg ttccctgatt
     2641 gagcacggtt gaccaggtct cggcgccgtt gagcgagacg gtggcgccgc cgtcgtggcc
     2701 gatgatcatg cgccgggtgt tgttggggtc gatccagagg tcgtggttgt cggcgtgcgg
     2761 cgtcgggatc tgggtgaagg tcttgccgcc gtcggtcgac ttataggtgt agaagttgag
     2821 cacccagacg gtctcggcgt cctccgggtc ggcgaagatg tgggtgaagt accaggggcg
     2881 ctgcagcagg ttgcgatcgt cgctgacgag cgtccaagtg tcgccgccgt cgtcggagcg
     2941 gtagacgccg cccttctcgg cctcgaccag cgcccagacg cggccgccct gggccggtga
     3001 ggcgacgatg ccgatcttgc cgaagacgcc ttccggcagc cctgggttat ggctgatgtc
     3061 ctcccagctg tcgccgccgt cgcggctgcg gaagaggccg ctctcggggc cgccgccgac
     3121 cagcgtgtgc gggtagcggc gcgcctgcca gaaggcggcg tagaggttgc gcgggttggt
     3181 cgcatccagc gtcaggtcga tggcgccggt ctcctcgctg cggaagagca cctgctccca
     3241 gtgctcgcca ccgtcggtcg agcggaagat gccgcgctcc ttgttcggtc cgtgagcgtg
     3301 gcccagcgcg gcgacataga ccaggtcggc gtcgtcggga tgcacgcgca ccttgctgat
     3361 gttacgtgtc tcgaccagcc ccacgttgcg ccagctcttg ccgccgtcgg tcgtcttgta
     3421 gaccccgtcg ccgtgcgaga cgttgccgcg aatcgtcgcc tcgcccatgc cggcgtagag
     3481 gacgttgggg tcggactcgg cgatggccag cgcgccgacc gaggcacgct tgaagtagcc
     3541 gtcggagatg ttgcgccagg tcatgccggt gtcgttgctc ttccagacgc caccgccggt
     3601 cgagccgaag tagaaggtgg cctgctccca ggggtggccg gcgacggcga tgacgcggcc
     3661 gccgcgaaac gggccgacga ggcgatagcg tagacccggc cagcgctccg ccgcctgccc
     3721 gttgctcgtg ccttcctgca tacctgtttc tccctccctg ctgtgcggga actactgcga
     3781 acacgatgag tcatgacaca actgctaccc catacggatg agagatcctt cggccgcggc
     3841 ctcacgatga cagagaagag tgcgccgtgt tccctgtcat cgcgagccgc ggcgaaggat
     3901 ctctcatccg ttaccccgcc ccaagacgtt gtcactggct gtcgacgccc cgcactcacc
     3961 cgcgcacccg cctcaactca ggcggacctc ggcgcgatcg gacccgacct cgatctccgt
     4021 gccgaagggt cgcgagagcg cggcgaagcg ctccagaata acgcctgcct gctcgccctc
     4081 ggccaggcgc ggccgcccgc gtcggctggt cggctggccg tggccgagcg tcaccgggta
     4141 gagcgcgatc tccttgagcg cgccgccctc gaagcggagg agcggcacca gcgtctccca
     4201 gtagcggctg tcggccggga agcccttggc gccgtcctcc gagcgctggc gatagacctc
     4261 gcccggtgtc aggttcggat cgacgcggaa ctgctcgtag tcgtcggccg ggagcttgta
     4321 ggtcaggtcg ttctgggcga tgaagttgcc caggctgtag aagatcggct tgccctgata
     4381 gagctccagg ccgcgcaaga ggtgcgagcc gtgaccgacg accaggtcgg cgccggcctc
     4441 gatcatctgg cgggcgaagt cgcgcgcgaa ggccgccgga tcctccgtgc tggccccttc
     4501 ctcgtggtgg tgcagactga ggatgaccag atcggc
//
LOCUS       DASUBI010000362         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_920457, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000362 DASUBI010000000
VERSION     DASUBI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920457"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFU72_06980"
     CDS             complement(<1..526)
                     /locus_tag="VFU72_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5423265.1"
                     /translation="MERRVFRIDLGYDGADFAGSQVQPGVRTVQGVVEEALARLTDEP
                     VRLALAGRTDRGVHAVGQVASGSLRWERPVEALAAGLRALTPPDVAIYRVALAPAGFH
                     ARYDAVAREYRYRIWSGAPPPLLLRGAVWHQRRPLDLQAMVRAAEALPGEQDFAAVAG
                     SGVGVPWAEIDRRRR"
     gene            complement(549..902)
                     /gene="rplQ"
                     /locus_tag="VFU72_06985"
     CDS             complement(549..902)
                     /gene="rplQ"
                     /locus_tag="VFU72_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5423266.1"
                     /translation="MRHRVAGRKFGRNTNQRKALRRNLAVSLILHDRMTTTEAKAKTI
                     RPVVEKLVTLARTDDLHHRNLVMSRLADERATAKLFDVIAPRFEGHPGGYTRIYKLGP
                     RRGDAAPMALIEFIA"
     gene            complement(899..1918)
                     /locus_tag="VFU72_06990"
     CDS             complement(899..1918)
                     /locus_tag="VFU72_06990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5423267.1"
                     /translation="MLEIAQPRVQQTHSGENYGRFEIEPLEPGYGITLGNALRRILLR
                     SLPGAAIVRVRITDVWHEFSTIEGVREDVTEIVLNLKQIRLQTQLDNFREARASLYAR
                     GEGPVRAGDIDWPAEVEVVNPEQIVATLDNERARLEMDLVVMRGRGYQPAEAQEIFSI
                     GEIPVDAIFTPIEKVNYVVEHTRVGQMTDFDRLILEIVTDGTIDPVEALSTAAQILVD
                     HGRLIADFNRQPSEAADETARPMTEADNKPLSELGLSPRVLNALRSRHIERVGQVLDM
                     DPDQLLSIRNFGPRSLQELKDKLAEHGYTLEVPTGLGGEELEDNEALDDVTAEGADDE
                     EEQEE"
     gene            complement(2007..2420)
                     /gene="rpsK"
                     /locus_tag="VFU72_06995"
     CDS             complement(2007..2420)
                     /gene="rpsK"
                     /locus_tag="VFU72_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5423268.1"
                     /translation="MADRRRGAARRGAARGRRRERKNIPRGRAYVQSTFNNTIVTLTD
                     QAGNVIAWSSAGSSGFKGSRKSTPYAAQVAAETAARKAMEHGLRQVEVFVKGPGAGRE
                     MAIRSLQAAGIQVLSIADVTPVPHNGCRPPKRRRV"
     gene            complement(2520..>2699)
                     /gene="rpsM"
                     /locus_tag="VFU72_07000"
     CDS             complement(2520..>2699)
                     /gene="rpsM"
                     /locus_tag="VFU72_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5423269.1"
                     /translation="VEGDLRREVNMNIKRLMDIGSYRGLRHRRGLPVRGQQTKTNART
                     RKGPKKGIGLKKKGR"
BASE COUNT          394 a          999 c          847 g          459 t
ORIGIN      
        1 ccctccgcct gcggtcaatc tcagcccagg gcacacccac gccgctcccc gccaccgccg
       61 caaagtcctg ctcgccgggc agcgcctcgg ctgcccgcac catcgcctgc aaatcgagcg
      121 ggcggcgctg atgccagacc gccccccgca gcagcagggg cggcggcgcc cccgaccaga
      181 tgcggtagcg gtactcccgc gccaccgcat cgtagcgagc gtggaagccc gcgggagcga
      241 gcgcgacccg gtagatcgcg acgtccggcg gcgtcagcgc ccgcagcccg gctgccagcg
      301 cctccacggg ccgctcccag cgcagcgagc cgctggccac ctggccgacc gcgtgcaccc
      361 cgcgatccgt gcgccccgcc agcgccagcc gcaccggctc atcagtcagc cgcgccagcg
      421 cctcctccac caccccctgg acggtccgca cgcccggctg cacttgcgag cccgcgaagt
      481 cggcgccgtc gtagcccaga tcaatgcgaa agacgcggcg ctccatggcc gcgcctcgcc
      541 tcgccccgct aggcgatgaa ctcgatcaac gccatggggg cggcatcgcc ccggcgcggg
      601 ccgagcttgt agatccgggt atagccaccg ggatgaccct cgaaacgcgg cgcgatcacg
      661 tcgaacagct tggccgtggc gcgctcatcg gccagacgcg acatcaccag gttgcggtga
      721 tgcaggtcat ccgttcgcgc cagcgtgacc agcttctcga cgacaggccg gatcgtcttg
      781 gccttggcct cggtcgtcgt catgcgatcg tgcaggatca acgacacggc caggttgcgc
      841 cgcaacgcct tgcgctggtt ggtgttccgc ccgaacttcc gtccggctac acggtgcctc
      901 attcctcctg ctcctcctcg tcgtcagcac cctcagccgt gacgtcgtcg agggcttcgt
      961 tgtcttccaa ttcctcgccg ccgaggccgg tcggaacctc caacgtgtag ccgtgctccg
     1021 ccagcttgtc cttcagctct tgcagcgagc gcgggccaaa gttccggatc gaaaggagct
     1081 gatccggatc catgtccagc acctgcccca cgcgctcgat gtgccggctg cgcagcgcgt
     1141 tgagcacgcg cggcgagagc cccaactcag agaggggctt gttgtcggcc tcggtcatgg
     1201 gccgcgccgt ctcgtcggcg gcctcgctcg gctgccggtt gaagtcggcg atcaagcggc
     1261 cgtgatcgac cagaatctgc gccgcggtgc tgagcgcttc aaccggatca atcgtgccgt
     1321 cggtgacgat ctccagaatc agccggtcga agtcggtcat ctggccgacg cgcgtgtgct
     1381 cgacgacgta gttgaccttc tcgatcggcg tgaagatcgc gtcgaccggg atctccccga
     1441 tcgagaagat ctcctgcgcc tcggccggct ggtagccgcg cccacgcatc accacgagat
     1501 ccatctcgag ccgcgcgcgc tcgttgtcga gcgtggccac gatctgctcg gggttgacca
     1561 cctcgacctc ggcgggccag tcgatgtcgc ctgcccgcac cgggccctcg ccacgggcgt
     1621 agaggctcgc gcgcgcttcc cggaagttgt cgagctgggt ctgcaggcga atctgcttca
     1681 ggttcaggac aatctcggtg acgtcctcgc gcacgccctc gatcgtcgag aactcatgcc
     1741 acacgtcggt aatgcgtacg cgcacgatgg ccgcaccggg caaggagcgc agcaagatgc
     1801 gccgcagcgc gttgcccagc gtgatgccat agcccggctc cagcggttcg atctcgaacc
     1861 gtccatagtt ctcgccgctg tgcgtttgct gcacacgcgg ctgagcaatc tccaacaccc
     1921 gattccgcct cctacgccga ctctctgcga gccgccgcta ttaactcctg atgacgtatc
     1981 ccgattgctg gcggagcctc gcggcgttag acgcgccggc gcttgggtgg ccggcagccg
     2041 ttgtgcggaa ccggggtgac gtccgcgatc gagagcacct ggatgccggc ggcctgcagc
     2101 gagcgaatgg ccatctcacg cccggcgccc ggtcccttga cgaatacctc gacctgacgc
     2161 agcccgtgct ccatcgcctt gcgcgcggcg gtctcggccg cgacctgcgc cgcgtaggga
     2221 gtgctcttgc gcgagccctt gaagcccgag gaccccgcgc tcgaccacgc gatgacattg
     2281 ccggcctgat cggtcagggt cacgatcgtg ttgttgaacg tcgactgcac gtaggcacgg
     2341 ccgcgcggga tattcttccg ctcacgtcgt cgtcctcgtg ccgctccgcg ccgcgccgcg
     2401 ccgcgccgtc gatctgccat gtagcccggt tcctttcctg actgctactc tgaagcggcc
     2461 cacgagccgc cggattctga ttgcgcatag cgccggcacc tgtgcagcgt cggaaggacc
     2521 tagcggccct tcttcttcag gccaatgccc ttcttcggcc ccttgcgggt gcgggcgttg
     2581 gtcttggtct gctggccgcg caccggcagc ccacggcgat ggcgcagccc acgataggag
     2641 ccgatgtcca tcagccgctt gatgttcatg ttgacttcgc gccgcaggtc gccctcgac
//
LOCUS       DASUBI010000363         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1320795, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000363 DASUBI010000000
VERSION     DASUBI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320795"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFU72_07005"
     CDS             <1..709
                     /locus_tag="VFU72_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU5423270.1"
                     /translation="VVELDALLIDGVTGRAGAVAALQGVANPIAVARRILEASPHVLL
                     AGDGATLFAQEQGFPPADCVTEAQRQAWLSMQASGSLRDLGLNPFTGQPEPTKPADTV
                     GCVVYTSEGTAAGVSTGGLFFKHPGRVGDSAIVGAGAFASEAGAVACTGLGEAFHELL
                     LAQRVGAQLQQGVHPQSAVEEALHYLWERRQGIGGVIAVDRHGRCGLAHNGQSFAAAV
                     LLDGQLAPAVPVRVLAR"
     gene            842..1591
                     /locus_tag="VFU72_07010"
     CDS             842..1591
                     /locus_tag="VFU72_07010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU5423271.1"
                     /translation="MGVLEGRKALVTGASRGIGREIALAFAREGADVAVNYHRQEEAA
                     REVAAAIEALGRRALAVQASVAEVEPVRTMVARVLAEFGHIDILVNNAGFVTLAPVEA
                     MTVEQWDEMLATHLRGTFLVTRQVLPGMLERGSGRIINVSSQLGQIGRPWFAHYCAAK
                     AGIIGFTKALAREVATRGVLVNCIAPGPIETGIVPRVEGAPRVDYTSRLPIQREGTVE
                     EVAPTAVFLASDAATYYVGQTLGPNGGDVML"
     gene            complement(1620..2027)
                     /locus_tag="VFU72_07015"
     CDS             complement(1620..2027)
                     /locus_tag="VFU72_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423272.1"
                     /translation="METIGRDIAPFLTFMGRAEEAMNFYVSLFDHSEVLSITRYGADV
                     PGAEGMVMHATFSLRGRVFMCIDSPISHEWGFTPAISLWVPCESDEEIERLYRALSDG
                     GQVYMELGSYPFSAKFAWVGDRFGVTWQLSLDR"
     gene            complement(2419..2862)
                     /locus_tag="VFU72_07020"
     CDS             complement(2419..2862)
                     /locus_tag="VFU72_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423273.1"
                     /translation="MTTSTTGRRWLVGPEDGAEHLLLDRLELRAGRVVGLHKHDGEEA
                     HVVLSGTVRFLVDGVQRVCGPGEVGFAPIGAEHGFRALSDAVLVTVREQRLGSIAILL
                     EPDGSRREIPIYRNGPPWSNEPPAGAGPTPNEEIEAIYRTTAHLI"
     gene            3039..4616
                     /gene="hutH"
                     /locus_tag="VFU72_07025"
     CDS             3039..4616
                     /gene="hutH"
                     /locus_tag="VFU72_07025"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5423274.1"
                     /translation="MRQSGTATGDHPQGAPGVIPIDGEHLALEEVVRVARGGARVALE
                     PGAEAAVAASRALIERILEGGATVYGVNTGFGELSSVSIPTGQLTALQRNLLRSHAAG
                     TGPCLPRDVVRAMLLLRANALAKGYSGVRPVVVQRLLDLLNAGVSPQVPAQGSVGASG
                     DLAPLAHLSLVLIGEGEATVGDDPAVLTGAEALKRAGLEPLSLSAKEGLALINGTQLM
                     TAIGVLALHDAEVLLESAEVAAAASLEALRGTDTAFDAEIQAVRPQPGQARAAARLRA
                     LLAGSEILASHRDPKLDPRVQDPYSLRCTPQVLGASWDALAYVRRVLEIECNSATDNP
                     LVFPGSGRVLSGGNFHGQPLALALDFLKLAVAEVGSISERRTYLMLDPARSGLPPFLA
                     PAPGIESGFMVAQYTAAALVSENKVLAHPASVDSIPTSAGMEDHVSMGPIAARGAAEI
                     IANVTRVVAIELLCGAQGVEQHVPLRPGRGVVEAVRRLREVVPPLTHDRPLAPDIAQV
                     AKMIGAGEFRLADQTVA"
     gene            4809..>5331
                     /locus_tag="VFU72_07030"
     CDS             4809..>5331
                     /locus_tag="VFU72_07030"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871590.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5423275.1"
                     /translation="MTIDRNDQQQHPSGPRPVRAPRGTELRCRGWEQEAALRMLMNNL
                     DQEVAERPDELVVYGGTGRAARSWAAFDAIVRELERLENDETLLVQSGKPVGVLRTHE
                     DAPRVLISNAMLVPNWASWEQFRELEQLGLTMYGQMTAGSWIYIGTQGILQGTYETFA
                     AVAEKRFNGTLAGT"
BASE COUNT          828 a         1820 c         1884 g          799 t
ORIGIN      
        1 tgttgtcgag ctcgatgcac tccttattga cggcgttacc ggtagggcag gagcagtagc
       61 ggcactgcaa ggggtagcaa accctattgc cgtggctcgg cgcatccttg aggcctcgcc
      121 acacgtcctc ttggctggag acggagcaac cctcttcgca caggaacagg gcttccctcc
      181 agcagattgt gtcacggagg cccaacgcca ggcgtggctg agcatgcagg cgagcggatc
      241 gctgcgtgac cttgggctga atccgtttac gggccaaccg gaaccgacta aacccgctga
      301 cacggtcggc tgtgtcgttt atacgagtga aggcacggcc gccggtgtat cgaccggcgg
      361 cctgttcttc aaacacccag gacgcgtggg agactcggct atcgtcggcg ccggcgcgtt
      421 cgcttcggaa gccggtgcgg tagcctgcac gggactagga gaagcgtttc acgaattgct
      481 actagcgcaa cgggttggcg cgcaattgca gcagggcgtg cacccgcaga gtgcggtcga
      541 ggaagccttg cactatcttt gggagcggcg acaaggtatt ggcggagtga tcgcagtcga
      601 tcgtcatggt cgctgtggcc tggcccacaa cgggcagtca ttcgcggcag cagtcctcct
      661 cgacgggcag ctcgcccctg cggttccagt gcgggttctg gctcgctagc cggtcgcgtt
      721 agaccaagca gtcatcccag ccgcgcccgc aactgatgac agccaacgcg ccagcagatc
      781 aactccggac gcgtactcag tatcactgtc atcgagagaa taacttggaa aggattgcgg
      841 agtgggcgta ttagaagggc gcaaagcgct ggtgaccgga gcgagccgcg ggatcgggcg
      901 cgagatcgcg ctggccttcg cccgcgaggg cgccgacgtg gcggtcaact accaccggca
      961 ggaggaggcg gcgcgcgagg tggccgccgc catcgaggcg ctggggcgcc gggcactggc
     1021 ggtgcaggcc agcgtggccg aggtcgagcc ggtgcgcact atggtcgcgc gcgtgctggc
     1081 ggagttcggc cacatcgaca tcctggtcaa caacgccggc ttcgtgacgc tggcgccggt
     1141 cgaggcgatg acggtcgagc agtgggacga gatgctggcc acccacctgc gcggcacctt
     1201 cctggtgacg cgccaggtgc tgccggggat gctcgagcgc ggctcgggcc ggatcatcaa
     1261 cgtctcctcg cagctcggcc agatcgggcg gccctggttc gcccactact gcgccgccaa
     1321 agccggcatc atcggcttca ccaaggcgct cgcgcgcgag gtggccacgc gcggcgtgct
     1381 ggtcaactgc atcgcgccgg ggccgatcga gacgggcatc gtgccgcgcg tcgagggggc
     1441 gccgcgcgtg gactacacca gccgcctgcc gatccagcgc gaggggacgg tcgaggaggt
     1501 cgcgcccacc gccgtctttc tggcctcgga tgccgcgacc tactacgtcg ggcagacgct
     1561 cggtcccaac ggcggcgacg tcatgctctg agccagcggc gagatcgggg tgcgcacgat
     1621 caccgatcga gcgagagctg ccaggtcacg ccgaagcggt cgccgaccca ggcgaacttc
     1681 gcgctgaagg ggtagctgcc cagctccatg tagacctgcc cgccgtcgga gagcgcccgg
     1741 tagaggcgct cgatctcctc gtcggactcg cagggcaccc acagcgagat cgccggcgtg
     1801 aagccccact cgtgcgagat cggactgtcg atgcacatga acacgcggcc ccgcagcgag
     1861 aaggtggcgt gcatcaccat cccctcggcg cccggcacat cggcgccgta gcgcgtgata
     1921 ctgagcacct cggagtggtc gaagagcgac acgtagaaat tcatcgcctc ctcggcccgc
     1981 cccatgaagg tcagaaatgg cgcgatatcc cggccaatcg tctccacgcg tgtctcccct
     2041 ttccaccgag cgcaccggcg atgtgcaact tcgaggttac agattagccg cggatcgacc
     2101 acggcgctcc cgtcgtagaa gccgcggcgg cacgtgacgc gcggggctga agccgccgcg
     2161 ctagatcttc cgaagcctgc tgaagcaggc tgaagtttga gggtggtggc gcgtcgctct
     2221 ggcggcgcac gttactcgct cagaacgtgc gcccggggaa cggttctctc tgggtttgag
     2281 atggccccag cctgcttcag caggcttccg tagaaggcta gcgcggtggc ttcagccccg
     2341 cgcgtcacgc ggcatcgcac tcccaggatg gccaccctcc accggcactc cagccgcgaa
     2401 cacgccctac ccagcgccct agatcaaatg cgccgtcgtg cggtagatcg cctcgatctc
     2461 ctcgttcggc gtcgggccgg cgccggccgg aggctcgttc gaccagggcg ggccgttgcg
     2521 gtagatcggg atctcgcgcc ggctgccgtc cggctccagc aggatcgcga tgctgcccag
     2581 ccgctgctcg cgcacggtga ccagcacggc gtcgctcagt gcgcggaagc cgtgctccgc
     2641 cccaatcggc gcgaagccga cctcgccggg gccgcagacg cgttgcacgc cgtcgacgag
     2701 gaagcgcacc gtgccgctca ggacgacgtg ggcctcctcg ccgtcgtgct tgtgcaggcc
     2761 caccacgcgt ccggcccgca gctccagccg gtcgagcagc agatgctcgg cgccatcctc
     2821 aggccccacc agccagcgtc gtccggtcgt gctcgtcgtc atacgtcgct cctcgtcgaa
     2881 tgcgagctac cgtccactcg ttcctatttt cgggcaaacc gccgcgatgt accttaaact
     2941 tagacccgag cctgtgcggg cggttggacg ggagactgac tgggcaaacc cgccacgacg
     3001 atttggcccg cacgagcagc tagaaagaca ggaccagcat gcgacagtca ggcacagcca
     3061 ccggcgacca cccgcagggc gcgccagggg tgattcccat cgacggcgag catctggcgc
     3121 tggaggaggt ggtgcgcgtg gcgcgcggcg gcgcccgcgt ggcgctggag ccgggggccg
     3181 aggcggcggt cgcggcctcg cgggcgctga tcgagcggat cctggaaggt ggcgcgacgg
     3241 tctacggcgt caacaccggc ttcggcgagc tgagcagcgt ctcgatcccc accggccagc
     3301 tcaccgccct gcagcgcaac ctgctgcgct cgcacgccgc cggcaccggg ccctgcctgc
     3361 cgcgcgacgt ggtgcgcgcc atgctcctgc tgcgcgccaa cgcgctggcc aagggctact
     3421 ccggcgtgcg cccggtggtg gtgcaacgcc tgctcgacct gctcaacgca ggggtctcgc
     3481 cccaggtgcc ggcgcagggc tcggtgggag ccagcggcga cctggcgccg ctggcgcatc
     3541 tctcgctggt gctgatcggt gagggcgagg ccactgtcgg cgacgacccg gccgtgctga
     3601 ccggggccga ggcgctgaag cgtgccgggc tggagccgct gtcgctcagc gccaaggagg
     3661 gcctggcgct gatcaatggc acgcaattga tgaccgccat cggcgtgctg gcgctgcacg
     3721 acgccgaggt gctgctggag tcggccgagg tcgcggccgc cgctagcctg gaggcgctgc
     3781 gcggcaccga caccgccttc gacgccgaga tccaggcggt gcggccccag cccggccagg
     3841 cgcgagccgc cgcccggctg cgggcgctgt tggccggcag cgagatcctg gcctcgcacc
     3901 gcgaccccaa gctcgacccg cgcgtgcagg acccctactc gctgcgctgc accccgcagg
     3961 tgctgggcgc ctcctgggac gcgctcgcct acgtgcgccg cgtgctggag atcgagtgca
     4021 acagtgccac cgacaacccg ctcgtcttcc ccggcagcgg gcgcgtcctc tcgggcggca
     4081 acttccacgg ccagccgctg gccctggcgc tcgacttcct gaagctggcc gtggccgagg
     4141 tcggcagcat ttcggagcgg cgcacgtatc tcatgctcga cccggcgcgt agcggcctgc
     4201 cgcccttcct ggcgcccgcg ccggggatcg agtcgggctt catggtggcg cagtacaccg
     4261 ccgccgcgct ggtctcggag aacaaggtgc tggcccaccc cgccagcgtc gactcgatcc
     4321 cgacctcggc cggcatggag gaccacgtca gcatggggcc gatcgcggcc cggggcgcgg
     4381 ccgagatcat cgccaacgtg acgcgcgtgg tcgcgatcga gctgctctgc ggggcgcagg
     4441 gcgtcgagca gcacgtgccg ctgcgccccg ggcgcggcgt ggtcgaggcg gtccggcggc
     4501 tgcgcgaggt cgttccgccc ttgacgcacg atcgcccact ggctccggac atcgcgcagg
     4561 tcgcgaagat gatcggcgct ggggagttcc ggctggccga tcagacggtg gcgtaagtcc
     4621 cccggtaggg gcagacccct gtgtctgccc tcgtggcccg gtgccgggcg tggtcgtccg
     4681 tcaccggcgt tccccttccc ccgttgcatt gtcgtcggga gaaccgggca gacacggggg
     4741 tctgccccta cagtgggcgg ggaacggtgg caaacgaggg tgcgaaggcg ttctagagag
     4801 gctttctgat gacgattgac cgcaatgacc aacagcaaca tccgagcgga ccgcggccgg
     4861 tgcgcgcgcc gcgcggcacg gagctgcgct gccgcggctg ggagcaggag gccgccctgc
     4921 gcatgctgat gaacaacctc gatcaggagg tggccgagcg gcccgacgag ctggtggtct
     4981 acggcggcac cggacgcgcc gcgcgcagtt gggccgcctt cgacgccatc gtgcgcgagc
     5041 tggagcggct ggagaacgac gagacgctgc tggtgcaatc cggcaagccg gttggggtgc
     5101 tgcgcaccca cgaggacgcg ccacgggtgc tgatcagcaa cgccatgctg gtgcccaact
     5161 gggccagttg ggagcagttc cgcgagctgg agcagcttgg cctgacgatg tacggccaga
     5221 tgaccgccgg gagctggatc tacatcggca cccagggcat cctgcagggc acctacgaga
     5281 cattcgcggc ggtggcggag aagcggttca acggcacgct cgcgggcacg c
//
LOCUS       DASUBI010000364         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_164524, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000364 DASUBI010000000
VERSION     DASUBI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164524"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFU72_07035"
     CDS             complement(<1..529)
                     /locus_tag="VFU72_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423276.1"
                     /translation="MTDDQALDRLIDALNAGRLDPAAIDEPELAELLALARDLRRLGP
                     SAPAPVTEWPAADFPRQLAQGLAAALRPAPRPLPPPSLLDSGDAAPPLPVLNRHAHMD
                     KQLATVTERGPARRWLREGLRLAAAGVALALLTGLLAALLHDRGGQSAHIGSAGGQGS
                     RPAVTAPGTPSGTPGP"
     gene            complement(526..1068)
                     /locus_tag="VFU72_07040"
     CDS             complement(526..1068)
                     /locus_tag="VFU72_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423277.1"
                     /translation="MGEQQLLYRARQGDTEALEELCRREWQPVYRLVARAVSNRAEAE
                     DLTQEVFLRALRALDRYQETGVPFRAFLATIARNLIRTRWKSRRGGPVNLADWPELPG
                     DEDGPEETAIAASERAELRRALDTLSADYQTVLRLRLLDGRPTDEVAALMGRSPGAVR
                     VLQHRALTALRAVLQKGAML"
     gene            1512..>2036
                     /locus_tag="VFU72_07045"
     CDS             1512..>2036
                     /locus_tag="VFU72_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423278.1"
                     /translation="MSRPAHRPVRVAFVLLCLTLALTLVPFAGRQAAAAGPGALVAYL
                     DRGNNLWVSNPDGSSARQLTPGGFGAVAWSPDGSRLAAAGPYNGGQGVYLTSPDGSLK
                     LTPLSAGAKPAWAPDSGDLALINSGTIAVFSRDGVAVRSAAVGADTLRWSPNAHIVGY
                     TRIVADPYGTNCPVR"
BASE COUNT          262 a          748 c          726 g          300 t
ORIGIN      
        1 cgggccccgg cgtgccgctc ggcgtgccgg gcgccgtgac cgccggccgc gagccctgcc
       61 cgcccgccga gccgatgtgc gccgattgcc cgccccggtc gtgcagcagc gccgccagca
      121 gcccggtcag gagcgccagc gccacgcccg ccgccgccag gcgcagcccc tcgcgcagcc
      181 agcggcgggc cgggccgcgt tcggtcaccg tggccagttg cttgtccatg tgcgcgtggc
      241 ggttgagcac gggcagcggc ggcgcggcgt cgcccgagtc cagcaggctg ggcgggggga
      301 gcggccgtgg cgcgggccgg agcgccgccg ccagcccctg cgccagctgc cgggggaagt
      361 ccgccgcggg ccactccgtc accggcgcgg gtgctgacgg gccaagccgg cggaggtcgc
      421 gcgccagggc gagcagctcg gccagttcgg gttcgtcgat cgccgccggg tcgagccgcc
      481 cggcgttgag ggcgtcgatc agccggtcga gcgcctggtc gtcggtcata gcatcgctcc
      541 cttctggagc acggcgcgca gggccgtgag cgcgcgatgt tgcaggacgc ggacggcgcc
      601 ggggctgcgc cccatgaggg cggcgacttc atcggtcggc cgcccgtcga gcaggcgcag
      661 ccgcagcacg gtctggtaat cggctgagag ggtgtcgagg gcccggcgca gctcggcacg
      721 ctcgctggcg gcgatggcgg tctcctcggg accgtcctcg tcgccgggca gctccggcca
      781 gtcggccaga ttgaccggtc cgccgcggcg gctcttccag cgggtgcgga tcaggttgcg
      841 ggcgatggtg gcgaggaagg cgcggaaggg gacgccggtc tcctggtagc ggtcgagcgc
      901 gcggagggcg cgcaggaaga cctcctgggt cagatcctcg gcctcggcgc ggttggagac
      961 cgcgcgcgcg acgaggcggt agaccggctg ccactcgcgc cggcagagct cctccagcgc
     1021 ctccgtatcc ccctgtcgcg cccggtaaag gagctgctgc tcgcccatgt gaccccctct
     1081 caccactcta aaccacgcat tggcgcgggt gttacagcta gagaggagga tgagagcttg
     1141 gagacttcct ttgccgttcg gtgctgcgtg acgccggaga atcgaattcc cgggctgagt
     1201 ccacgaagcc cactaaaggg actgcgttta cgtttgccga tagcagcaca tcttcgccca
     1261 aatcgccaac tcacccttca tgtacacgcg atagcgacga cgcggcgaca atcgttctca
     1321 gccccttcag tgggcttttt ggactcagcc cgagaattcg attctcgggc gtcggcgggc
     1381 gcccgtgtcc caagggacgc ggccgccccc cgaacaaccc tcaccgccgc atattcgttg
     1441 tagtctgtgc gcccgtgtgg gaggcgggcg cgctgtggtg ctctatcccc tgggaatggg
     1501 aggtctcgcg catgtccagg cccgctcacc ggccggtgcg cgtcgcgttc gtgctcctct
     1561 gcctcaccct cgcgctgacg ctcgtaccct tcgctggccg ccaggccgcc gccgccggcc
     1621 ccggcgctct ggtcgcctat ctcgaccgtg gcaacaacct ctgggtcagc aaccccgacg
     1681 gctcctcggc gcgccagctc acccccggcg gcttcggcgc tgtcgcctgg tcgcccgacg
     1741 gcagccggct ggcggccgcc gggccctaca acggcggcca gggtgtctat ctcaccagcc
     1801 ccgacggtag cctgaagctc acgccactca gcgcgggagc caagcccgcc tgggcgccgg
     1861 acagcggcga cctggcgctg atcaatagcg gtacgatcgc cgtcttcagc cgcgacggcg
     1921 ttgcggtgcg tagcgccgcc gtcggcgctg acacgctgcg ctggtcgccc aacgcccaca
     1981 tcgtcgggta tacccgcatc gtcgccgacc cctacggaac caactgcccg gtgcgc
//
LOCUS       DASUBI010000365         7187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_521963, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000365 DASUBI010000000
VERSION     DASUBI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521963"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFU72_07050"
     CDS             complement(<1..565)
                     /locus_tag="VFU72_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEU5423279.1"
                     /translation="MSTKAATSSPAIALPKGGGALTGIGETFSPDLHTGTGNFSVPIA
                     LPPGRHGFQPELSLRYSSGSGNGPFGLGWGLNMPSISRKTSRGLPRYDEAIDTFIFAG
                     AEDLVPVASMPTCTYYRPRTEGLFARIMHVHSADDNYWEVRTTDGRVDLYGTPRPTTA
                     PATWRDPASVADPDRPEHIFTWQLTRTT"
     gene            complement(888..1067)
                     /locus_tag="VFU72_07055"
     CDS             complement(888..1067)
                     /locus_tag="VFU72_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423280.1"
                     /translation="MLQEQYEEVLPVFLIVGEFGVVFALWQRPDELPSHAVLATWALD
                     RNPDRAASPDFPKPG"
     gene            complement(1239..2594)
                     /locus_tag="VFU72_07060"
     CDS             complement(1239..2594)
                     /locus_tag="VFU72_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5423281.1"
                     /translation="MDTLPPAMITLVAPFAPLFSDRVWRHALVLVAGTLLAPGKRTVC
                     AALRAMGLSGTRQFARYHRVLNRATWSSLAVSRVLLGLLVAAFAPTGPVVVGVDETIE
                     RRWGAQIAAKGLYRDAVRSSKGYFVKVSGLRWLCLMLLVPLPWAERVWALPFLTILAP
                     SERHDRERGRRHKKLTDWARQALLLLRRWLPQRALVVVADSGYAAIELLTHCTRLVNP
                     ITVVTRLRLDAALYEPAPPRRPGQNGRPRKKGRRLPTLAARIADPVTAWTPVVVNWYG
                     EGARVVEVASDTAVWYHSGLPPVPLRWVLVRDPQGRFDTQALLCTDLAADPAQILAWF
                     VLRWQLEVTFEEVRRHLGVETQRQWSERAILRTTPALLGLFSLVTLWAHPRIGPTGDR
                     VRQAAWYHKPLPTFSDALALVRRELWGHLAFCMSDREADLVKVPRALIERFADALCYA
                     A"
     gene            2713..3602
                     /locus_tag="VFU72_07065"
                     /pseudo
     CDS             2713..3602
                     /locus_tag="VFU72_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            3679..4845
                     /locus_tag="VFU72_07070"
     CDS             3679..4845
                     /locus_tag="VFU72_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEU5423282.1"
                     /translation="MVDQGLVQAWAADLDALPARLGPRFGRAEPRRRVRAYGARLLSP
                     VERKNGWQVAEQAGEATPYGMQRLLAGAKWDAEAVRDDLHASVVEHLGDPEAVLIVDE
                     TGFLKKGTKSVGVQRQYSGTAGRIENCQVGVFLCYATPRGRTFLDRELYLPKEWANDA
                     VRREEAGVPEDVAFRTKPELARAMLARAFAAGVPAGWVTGDEVYGRDRRLRLWLEQQN
                     RPFVLAVAANEPLWCATPRGPQQVGAADIAAAIRPGAWERVSAGDGAKGPRLYDWARV
                     RRARLDEPGRGHWRLVRRSLDDPAAFAFSVVFAPAATALAALVRVAGTRWAVEEGFEI
                     AKGEVGLDHYEVRRWEGWYRHITLALVAQAFLAVVRAGAAGAQKGGAPPCWPPR"
     gene            4827..5021
                     /locus_tag="VFU72_07075"
     CDS             4827..5021
                     /locus_tag="VFU72_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423283.1"
                     /translation="MLAAALIPLTVPEVRRLLWRLVWHAVPPPDFVIAWSRWRRRHQA
                     RARRSHYKRRLVRLPGQVQL"
     gene            5296..5973
                     /locus_tag="VFU72_07080"
     CDS             5296..5973
                     /locus_tag="VFU72_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423284.1"
                     /translation="MHIARVRLTADNLPAQRAFYAGTLGLRVVATEAAAFTVRAGDSL
                     LTFAQGHAGGPYHLAFNVPRARFQEAQAWLAARAPLLTRDGADEFAFTSWAARACYCA
                     DPAGNILECIARQTIADGAAAGPFGPAALRCVSEIGLAVVAVPAAAAALSAQFGLPPY
                     GDVSATFAALGDERGLVILAAIGRPWLPTTDARAAVAPVAVTIAGGRRGQYRLSGYPY
                     VIEAVAR"
     gene            6286..6744
                     /locus_tag="VFU72_07085"
     CDS             6286..6744
                     /locus_tag="VFU72_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423285.1"
                     /translation="MVAHVDETTDILLIEDSAADTRLVREALRACPHAHLHCLPNGET
                     ALAALQHRVPVSDGAIQPGFILLDLNLPRLDGRAVLRALQADPVLARIPVVVLSTSAH
                     SADVAACYDLGANAFVTKPLDLDAFLATVQAIARFWCRVVRLPARVPPSR"
     gene            complement(6816..7001)
                     /locus_tag="VFU72_07090"
     CDS             complement(6816..7001)
                     /locus_tag="VFU72_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423286.1"
                     /translation="MPQPEATSPPGVRYLRKPFGLAELVREASALLERPDHPIAPAWP
                     AERHAAHSRGCAVAAGD"
BASE COUNT         1103 a         2428 c         2585 g         1071 t
ORIGIN      
        1 ccgtcgtgcg ggtcagctgc caggtgaaga tatgctcggg gcggtcgggg tctgcaacac
       61 tcgccggatc ccgccaggtc gcaggtgcag tcgtgggccg cggcgtgccg tacaggtcga
      121 cgcgcccgtc cgtggttcgc acttcccagt aattgtcgtc cgcgctgtgg acgtgcatga
      181 tgcgggcaaa cagcccctcc gttcttggcc ggtagtaggt gcaggtcggc atgctcgcca
      241 cgggcacgag atcctcggcg cctgcgaaga tgaacgtgtc gatagcctca tcgtaccggg
      301 ggagccctcg cgacgtcttg cggctaatac tcggcatgtt cagcccccag cccaagccga
      361 aggggccgtt accggaacca gaactgtaac ggaggctcag ctcaggctgg aagccgtgac
      421 ggcctggtgg cagggcgatc ggtaccgaga agttgccagt acccgtgtgc aggtcgggtg
      481 agaaggtctc gccgataccc gtcagcgcgc cgccaccttt cggaagcgcg atggccggac
      541 tcgatgtcgc tgccttggta ctcatccgtg cgctccttgt ggctgtgatt gggcgaagaa
      601 tcgccgatca ccagcaggta aagactgatt caccactgcg aagtgcaatt ggctacccgc
      661 cgtccgcagc cgcttaccga gcgcagcgag gcggcatgtc cgcgctacgg agctgccgac
      721 gacagcttcg tcacctgggc gagccagcca cagtctggac ggtatctctc gacactgggc
      781 tgttaggtgc tgccacagtg tagcccccca gcccctgtct ccggtacgct atcagggcca
      841 gcctccttga ccagccatcg tgtgccggcg cgcggcggca ctcggcatta ccctggctta
      901 gggaaatccg gcgacgcggc gcgatccgga ttccggtcga gtgcccaggt tgccaggacc
      961 gcatgcgatg gcaattcatc cggcctctgc cacagagcga agacaacgcc gaactcgccg
     1021 acgatgagaa atacaggcag tacttcctca tactgctcct gcagcatgtg catggctgcg
     1081 ctgtgcgggt ttaccgcgtt cgatcgttgg tccagatccc gctaaggaca cggtcctcac
     1141 gctcgaataa acgagcagta cgtcaatttt cggacaagag tcgtagatcg ataatttaca
     1201 ctaccaccct aagctcgact ttggccgttc gtcgccgatc aggcggcgta gcagagcgcg
     1261 tcggcgaagc gctcgatgag tgcgcggggg actttcacga gatcggcctc gcgatcggac
     1321 atgcagaaag ccaggtggcc ccagagttcg cggcggacca gggcgagcgc gtcggagaag
     1381 gtcggcagcg gcttgtgata ccacgcggcc tgccgcacgc ggtcgccggt cggccctatg
     1441 cgtgggtgcg cccacagcgt gaccagcgag aagagaccga gcaaggccgg cgtggtccgc
     1501 aggatggcgc gctcggacca ctggcgctgg gtctcgacgc cgaggtggcg gcgcacttcc
     1561 tcgaaggtga cctccaactg ccagcgcagc acgaaccagg cgaggatctg cgccgggtcc
     1621 gccgccaggt cggtgcagag caacgcctgc gtgtcgaacc ggccctgcgg gtcacggacc
     1681 agcacccagc ggaggggcac cggcggcagg ccgctgtggt accacaccgc cgtgtcggag
     1741 gcgacctcca cgacgcgcgc gccctccccg taccaattga cgacgacggg cgtccacgcc
     1801 gtgacggggt cggcgatgcg cgccgccagt gtgggcaggc gtcgcccctt cttgcggggg
     1861 cggccgttct ggccgggccg gcgcggcggg gccggctcat agagcgcagc gtcgaggcgc
     1921 agccgcgtga cgacggtgat cgggttgacc aggcgcgtac agtgggtcag cagctcgatg
     1981 gcagcgtagc cgctgtcggc gacgacgacc agggcgcgtt gcgggagcca gcgccgcagg
     2041 agcagcagcg cctgccgcgc ccagtcggtg agcttcttgt ggcgccgccc gcgctcccgg
     2101 tcgtgccgct cggacggagc gaggatggtg aggaagggca aggcccagac ccgctcagcc
     2161 caggggagcg gcacgaggag catcaggcac aaccagcgca gcccgctgac cttgacgaag
     2221 tagcccttac tggagcgcac ggcatcccga tacagcccct tggccgcgat ttgcgcgccc
     2281 cagcggcgct cgatcgtctc gtccaccccg acgaccaccg gccccgtggg ggcgaacgcc
     2341 gcgacgagca ggcccagcaa cacccggctg actgccagac tggaccaggt ggcgcggttg
     2401 aggacgcgat gatagcgagc gaactgcctg gtgccgctca ggcccatcgc gcgcagcgcg
     2461 gcgcagacgg tgcgcttgcc cggagccaag agtgttccgg cgaccagcac cagcgcatgc
     2521 cgccagacgc gatcggagaa gagtggcgcg aagggggcca ccagggttat catcgcgggc
     2581 ggtagggtat ccatcgtcat ctcctcttgc aagagtgatg acgagcatac cctatcgctc
     2641 ttcatgctcc aaagggccaa agtcgagata acagcctatc cggaaagtga ggcgctgtgg
     2701 tagcatcctc ctatgaatac gcccaacgcc gcgccagcgg ccccccaatc acgcaaagac
     2761 gagccgacga tttggcgtgt ggacgacgcg ctgtgggccg aactccagcc gctcctcgtg
     2821 gtcgacaagc cacgcaagaa gcctgggcgg ccccgccacg acgaccgcgc gatcttcgat
     2881 gggctgatct ggctggcgcg gacgggcagc cagtgggccg cactcccgcg cgagtttggg
     2941 gccaaatcta cggtccacgc gcgtttcttg gaatgggtcg agcacggctg cttccaccgg
     3001 gcctgggcgg tcctcctcgc acggtacgat ggcgaggtgg gcctcgactg gcagtggcaa
     3061 gccgccgatg gctgtttggt caaggcgccg ctgggcaaaa aaggggcgct ggtgaggcac
     3121 aggcgaccgg ggccaacccg accgaccggg gcaaatgtgg cgtcaagcgc cacctcttga
     3181 ccgacggcca gggcgtgccg ctcgccacgg tgctctcggg ggcgaatcgc accgacatga
     3241 agaaactggg tgacctgctg gacgcgcagc tggtcgaagc cccgccggac cgggcgcggc
     3301 atctgtgtct ggaccggggc tacgactatg cggcctgccg cgagacggcg cgcgcgcatg
     3361 gctacaccgc gcatatcccg ccgcaggcga gcgcagcgca gcccctgccg ccaccgggcc
     3421 atcccgaccg ccacctacca cgccgctggg tggtggaggt cgcgcatagc tggtgcaacc
     3481 gcttccgccg gttgctcatc cgctgggaga agcacgccga cacctatctc gccttcgtcc
     3541 aactggccgc ctgcctcatt atctaccgca aactccgcca tgctcggtca ctttccggat
     3601 aggctgtaat tctacagttg cacttgcgtg gttggttgcg catcatgccc ggcgaggaga
     3661 ggggacgtga tggcggcgat ggtagaccaa gggctggtgc aggcgtgggc ggccgacctc
     3721 gacgcgctgc ctgcgcggct ggggccgcgg ttcgggcggg cggagccccg ccgtcgggtg
     3781 cgcgcgtatg gcgcgcgcct gctcagcccg gtggagcgca agaacggctg gcaggtggcg
     3841 gagcaggcgg gggaagcgac gccgtatggg atgcagcgcc tgctggccgg ggcgaagtgg
     3901 gacgccgagg cggtgcgcga cgacctgcat gcctccgtgg tcgagcacct gggcgacccg
     3961 gaagcggtgc tgatcgtcga cgagaccggc ttcctgaaga agggtaccaa gtcggtcggg
     4021 gtgcagcggc agtacagcgg cacggcgggg cgcatcgaga actgccaggt cggcgtcttc
     4081 ctctgctacg ccacgccgcg cgggcgtacc ttcctcgacc gggagctgta tctgcccaag
     4141 gagtgggcca acgacgccgt gcggcgggag gaggccggcg tcccggagga tgtggcgttc
     4201 cgcaccaagc ccgagctggc gcgggcgatg ttggcgcggg ccttcgcggc cggggtgccg
     4261 gcggggtggg tgacgggcga tgaggtctac gggcgtgacc ggcggctgcg gctgtggctg
     4321 gagcagcaga accggccgtt cgtgctggcg gtggcggcga acgagccgct gtggtgcgcc
     4381 acgccgcggg ggccgcagca ggttggggcc gccgacatcg cggcagcgat ccggcccggc
     4441 gcgtgggagc gggtctccgc gggcgacggg gccaaggggc cgcggctgta cgactgggcg
     4501 cgggtccgcc gcgcgcgcct ggacgagccg gggcggggcc actggcggct ggtgcgccgc
     4561 agcttggacg acccggccgc gttcgcgttc tccgtcgtct tcgctcccgc cgcgaccgcg
     4621 ctggcggcac tcgtgcgggt ggccggtacg cgctgggccg tggaggaggg cttcgagatc
     4681 gccaaagggg aggtcgggct ggaccactac gaggtccgcc gctgggaggg ctggtatcgg
     4741 cacatcacgc tggcgctcgt cgcccaggcg ttcctggcgg tggtgcgagc gggcgccgcc
     4801 ggggcgcaaa aggggggcgc gccgccgtgc tggccgccgc gttgatcccg ctgacggtgc
     4861 ccgaggtgcg ccgcctgctg tggcggctgg tgtggcacgc ggtcccgccg ccggacttcg
     4921 tgatcgcctg gtcgcgctgg cgccgacgcc accaggcgcg ggctaggcgc agccactaca
     4981 agcggcgtct ggtgcgcctg cccggccaag tgcaactgta ggagtaaggc gtttatggtc
     5041 aaggtcgcga tgagtcggct gcctctgtcc cggcactgcc ggcaccagaa ccgagtttct
     5101 tgcggagtca ttcgccagct gtcgttccaa tcaggtagct gcccgtgctt cgccagccag
     5161 acaactatcg ccatgacgag aacccacgcg aggactccag gaaaatgatc acgaaacggt
     5221 ggcgaaaacc tcgggaaacg gcgagaatag cgatgccagc ggcgacggac gaaccccgcg
     5281 tcgagggggg acacgatgca catcgcgcga gtgcgcctga cggcagacaa cctcccggcg
     5341 cagcgggcct tctacgcggg cacgctcggg ctgcgcgtcg tcgccacgga ggcggccgcg
     5401 ttcaccgtcc gcgcaggcga ttcactcctc accttcgcgc agggtcacgc gggggggccc
     5461 taccacctcg cattcaacgt cccgcgtgct cggttccagg aggcgcaggc ctggctcgcg
     5521 gcgcgggcgc cgctgctcac ccgggacggc gcggacgagt tcgcgttcac ttcgtgggcc
     5581 gcccgcgcat gctactgcgc cgatccggcc ggcaacatcc tcgagtgcat cgcccgccag
     5641 acgattgcgg acggagcggc ggcggggccg ttcgggccgg cggcgctgcg gtgcgtgagt
     5701 gagatcgggc tggcggtggt cgctgtcccg gcggcggccg cggccctgtc cgcgcagttc
     5761 ggcctcccgc cctacggcga cgtgtcggcc accttcgccg ccctcggcga cgagcgcggg
     5821 ctcgtcatcc tcgccgcgat cggccggcca tggctgccaa cgaccgacgc ccgcgcggcg
     5881 gtcgcgccgg tcgccgtcac gatcgccggg ggcaggcggg gccagtatcg gctgtcgggc
     5941 tatccctatg tcatcgaggc ggtcgcgcgc tgaggacggc cgccagccgc ctgaggtacg
     6001 gcgaccgcag ttggtcgacc gcgcgcatcc tggcgccggc ggcggtgccg gcggcgccaa
     6061 catccgcagc gccacccttc cttgcgcgta cgctgggcca ggtccgtgcc agtgcgcgtg
     6121 accgggtgcg gcggcacgcc gccgggacac ccagccgcgt cgcgttggcc gcgaccgttt
     6181 acccaccagt gacccccgac aacctagccg gcaagggcat gcggcgatag tacacgctaa
     6241 cgaaacggtg acgcggcgag cgcggacaga agagaccatg aaggcgtggt ggcgcacgtg
     6301 gatgagacga ccgatatttt gctgatcgag gatagtgcag cggacacgcg cctggtgcgc
     6361 gaagccctgc gcgcgtgtcc ccacgcgcac ctgcactgcc tgcccaacgg cgagaccgcg
     6421 ctcgccgcgc tccagcatcg tgtgcccgtg tcggacggcg ccatccaacc tgggttcatc
     6481 ctactggacc tcaacctgcc gcggttggac gggcgggcgg tgctgcgcgc ccttcaagcc
     6541 gacccggtgc tggcgcgcat ccccgtcgtc gtgctcagca cctcggcgca ctcggcggat
     6601 gtggcggcct gttatgacct gggagccaac gccttcgtca ccaagccgct cgacctcgac
     6661 gcctttctgg ccaccgtgca ggccatcgcc cgcttctggt gccgcgtggt ccgcctgccc
     6721 gcgcgggtgc cgccgtcgcg ctgaggcccg tgcggtacca cctcgccctc gccgtcggcc
     6781 agcgcgacga gcgtgagggt tgctcgcgtg gcgcctcagt ccccggcggc gacggcgcag
     6841 ccccggctgt gcgccgcgtg gcgctccgcc ggccaggccg gcgcgatggg gtggtccggc
     6901 cgctcgagga gcgcgctcgc ctcgcgcacc agctccgcca gcccaaaagg cttacgcagg
     6961 tagcggacgc caggcggact ggtggcctcc ggctggggca ccacggtact ctgtatacgg
     7021 caacgaaaac tgggttctgc ggatcatccg gtgacggcgg ggtcgtccca tccgctcggc
     7081 tccgcaaccg gcgggacacg gtcgcgtgtg ggagaatgct ctccaccagg atgctgtccg
     7141 tcgccccgga ggtcgccatg accgtgccaa cactcgtgcc cgactgc
//
LOCUS       DASUBI010000366         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_805137, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000366 DASUBI010000000
VERSION     DASUBI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805137"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFU72_07095"
     CDS             complement(<1..367)
                     /locus_tag="VFU72_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423287.1"
                     /translation="MNLLAWSLTDAERVVLAAALDALIPPEGSFPRPSECGVIDEFIL
                     RRVPPPGAQPVPYPGLDADGLRALLARLTAESAAMTAGLVRLEQEHPAEFLALWRLAA
                     YGYYSRPATIAAIQRELAPA"
     gene            592..951
                     /locus_tag="VFU72_07100"
     CDS             592..951
                     /locus_tag="VFU72_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423288.1"
                     /translation="MTANAALFVGWGQAIPGREQKALQVFNEAIQYWSKLQQQGMIES
                     FEPFLLEPHGGDLAGFCLLRGQHETLAQLRTTPEFIQLNARAGLVVANLGVVSAFTGE
                     ALQQQFADFGQFAAELA"
     gene            complement(963..2756)
                     /locus_tag="VFU72_07105"
     CDS             complement(963..2756)
                     /locus_tag="VFU72_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HEU5423289.1"
                     /translation="MNRRSATFDQVSSTRPDGARPRSADELRASLARLDRRPYPAYKE
                     LRGAWSLGAVTLFIDHVQGDPFAAPSRVRLRVGREHGLDPALDGTDPARRVALADFLA
                     RRGAARIAAQSHRGRQGSGKSGEVRIDVPGQEVLERSACRVTGDWAELRLSAGLPAAG
                     RTILGREATELLLDTLPAFAWQALRLDSDATATARRHCDTVEDARALRQQLRERGLVA
                     FVADGATLPRTSGVSQRPRAGGVPFNSPPELRVTLTAPHAGAVSGLGVPEGLTVIVGG
                     GYHGKSTLLAALARGVYDHAPGDGRERVVSRADAAVIRAEDGRRVATVDISPYIGTLP
                     SGDSTARFSTDNASGSTSQAANIVEALEAGCRLLLMDEDTCATNFLIRDARMQRLVPA
                     GHEPITPFVDRVRDLWTGPGVSTILALGGSGDYLGLADTVIWMDAYEPHDVTARAHQV
                     AAEVGPARQPERVIPLGLPPARVPDPGSISAERAGRTRVRARGTEEIAYGAEEIDLRA
                     VEQIVDPSQTRAIGALLAHAVARGHFDGRRTIAEGLARLDADLDRAGLDLLAPQPGEH
                     PGDLARPRMLEVAAALNRLRSLRVALTSPPG"
BASE COUNT          464 a         1002 c         1026 g          467 t
ORIGIN      
        1 aggccggagc cagttcccgc tggatggcgg cgatggtcgc cggtcgcgag taatagccgt
       61 aggcggcgag gcgccacagc gccagaaact ccgctgggtg ttcctgttcg agccggacga
      121 ggccagccgt catcgccgcg ctctccgccg tcagtcgtgc cagcagcgcc cgcagcccat
      181 cggcgtcgag gccgggataa ggcacaggct gggcgccggg cggcggcacg cggcgcagga
      241 tgaactcgtc aatcacgccg cactccgagg ggcgcggaaa actgccctcc ggcgggataa
      301 gggcgtcaag cgcggcagcg agcacaacgc gctccgcatc ggtcagcgac cacgctagca
      361 gattcacgct ttccttctct cgttcaatag ttgtcgccgc ttctctcgcg ccatcctaca
      421 ctgctaggcg cgctgccgct acttgcttgt cgtctacctg attgcggagg gcgcggtata
      481 ggtggttttc cggatggttc cggcgctcga attggcgtat ggtaagagtg gcctgatccg
      541 cgcgaaacgt ttgttgcgag tgtctgcggc aacgctgaaa ggagaacggc gatgacggca
      601 aatgcggccc tgttcgttgg ctggggccag gcgattcccg gccgcgagca gaaggcgtta
      661 caggtcttca acgaggccat tcagtactgg agcaagttgc agcaacaggg catgatcgag
      721 agtttcgagc cgttcttgct ggagccacac ggcggcgatc tcgctggttt ctgtctctta
      781 cgcgggcagc acgaaacgct ggcgcagttg cggacgacgc cggagtttat ccagctgaac
      841 gcgcgggctg gtctcgtcgt ggcaaatctg ggcgtcgtca gcgccttcac cggcgaggcg
      901 cttcaacagc agttcgccga tttcggccag ttcgcggcgg aactggcctg acagcgccac
      961 cctcaccccg gcggcgaggt gagggccacg cgcaggctcc gcagccggtt gagcgcggcg
     1021 gcgacttcga gcatccgggg acgcgccagg tcgccgggat gctcgccggg ttgcggcgcg
     1081 agcaggtcga gtccggcgcg gtcgaggtcg gcgtcgaggc gggccaaacc ttctgcgatg
     1141 gtccgccggc catcgaagtg cccgcgcgcc accgcgtgcg ccagcagggc gccgatcgcg
     1201 cgcgtctggc tggggtccac gatctgctcg accgcgcgca ggtcaatctc ctccgcgccg
     1261 tacgcgatct cttcggtgcc gcgcgcccgt acgcgtgtgc ggcccgctcg ctccgcgctg
     1321 atgctgccgg ggtccggcac gcgcgctggc ggcaggccga gcggtattac ccgctccggc
     1381 tggcgtgccg gcccaacctc ggcggccacc tggtgcgcgc gggcagtgac atcgtgcggc
     1441 tcgtaggcgt ccatccagat tacagtgtcg gcgagaccca ggtagtcacc agacccaccc
     1501 agcgccagga tggtcgagac gccggggccg gtccagaggt cgcgcacgcg gtccacaaag
     1561 ggtgtgatcg gctcgtgacc ggccggcacc aggcgctgca ttcgcgcgtc gcgaatcagg
     1621 aaattcgtcg cgcaggtgtc ctcgtccatc aacagcaagc ggcagcccgc ttccagcgcc
     1681 tcgacgatat ttgcggcctg cgacgtgctg ccggacgcat tatcggtgct gaagcgcgcg
     1741 gtcgagtcgc cggacggcag tgtgccgatg tagggcgaga tgtccaccgt ggcgacgcgc
     1801 cggccgtcct ccgcgcggat aactgccgcg tcggcgcggc tgacgacgcg ctcgcggcca
     1861 tcgccgggcg catggtcgta gacgccgcgc gccagcgccg ccagcaaggt tgacttgccg
     1921 tggtagccgc cgccgacgat gaccgttagc ccttccggca cacccaggcc actgactgcg
     1981 cccgcgtgcg gcgcggtcag tgtgacccgc agttcgggcg ggctgttgaa cggcacaccg
     2041 ccggcgcgcg ggcgctgtga gacgccactg gtgcgcggca aggttgcccc atcagcgaca
     2101 aaggctacca ggccgcgctc gcgcaactgc tggcggagcg cccgcgcgtc ctccacggta
     2161 tcgcagtgcc gccgcgcggt cgcagtagcg tctgaatcaa ggcgtagcgc ctgccaggca
     2221 aaggctggca gcgtatcgag caacagctcg gtggcctcgc gtccgagaat tgtccgcccg
     2281 gcggcgggca gacccgcgct gaggcgcagt tcggcccagt cgccggtgac gcggcaggcg
     2341 ctccgctcca gcacctcctg gcccggcacg tcaatccgca cttcgcccga tttaccggac
     2401 ccttgccggc cccggtggct ctgcgctgcg atcctggccg cgccgcgccg ggccagaaag
     2461 tcggccagcg ccacgcgccg cgcgggatcg gtaccgtcga gtgccgggtc aagtccgtgt
     2521 tcacggccta cacgcagtcg cacacgcgac ggcgcggcga aaggatcgcc ctgcacatgg
     2581 tcaatgaaca gcgtcaccgc accaagcgac cacgccccgc gcaactcctt gtacgccgga
     2641 taggggcggc gatcgagccg cgccagcgat gcgcgcaact cgtcagcgga acgcggacgt
     2701 gccccatccg gtcgcgtgga cgaaacctga tcgaaggtgg cggagcggcg gttcatcgtt
     2761 ctccctagat gcacatgact gtgtcaccaa gcctctggcg gttatagccg attggcggcg
     2821 gcgggcgcaa aagccactgg gggtcgaggg cgggtgaaat cctaacccac gcaggcgggt
     2881 ttcgtggtcc cgcagggcca ttcaggcgcg acttacggtc accggccaac gcccgaagat
     2941 tgattctttt cagcctcaa
//
LOCUS       DASUBI010000367         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_576856, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000367 DASUBI010000000
VERSION     DASUBI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576856"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VFU72_07110"
     CDS             <1..1676
                     /locus_tag="VFU72_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423290.1"
                     /translation="ALGISLVAGVTAGGVAAGDAAGGKLFAGSTPRTDGTTVVYETMG
                     NGNWDVVGASLADRQQFPLALGPSQAMYADIEGDVAIWQETNGRDDYDIVGKHINSNT
                     SFTIAASANSEVYPALSDGWVAFVSAPHIFAETEVQELQVRSVVTMDPARTLDKAALA
                     GGPSGGFLRPAISGKRVAWVRLEQTSLHVVHWQLKTQRLDETSATVIAEADLDIGGPL
                     GALAMPSVDLSGDILVYSADLRLTVVNLATGERHEIAAAQEADYHPAQNPTTDGRFVF
                     WQDYHGTGDTGTMIDMLQHGTLTAAIGGYDLLTGSRFTAVANQGYNVDPYARHGLLTF
                     ARKESVAQGESQVYALPVASLLPSAPQSDPGDPNVSYFPETGHWLGGEIRDFWKLSGG
                     LPVFGYPLTAEFDEGGFTAQYFERQRFEVHPEYQGTPYAVELGLLGVEDAAKRGLLDT
                     TPFQRLSEDAPRTAPCVYFAATGHTVCANFLNYWQSHGLEFGDPGISYRESLALFGYP
                     ISQPFTDPATGLTVQYFERARFEWHSENTAPYNLLLGRLGFDRLSERGW"
     gene            1831..>2330
                     /locus_tag="VFU72_07115"
     CDS             1831..>2330
                     /locus_tag="VFU72_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423291.1"
                     /translation="MVRELTREERIEPLEQRWALATGEEFQVAAAKRDPAAFAPLYER
                     YVDAIYRYAYRRVGSHAEAEDVTAQTFQQALAALPNFEWRGVPFGAWLYRIAGHVIVQ
                     RGRRSSRELVVEDLRVHLDRGDRADDEDPARLFEARAEAGELVAAVRRLPRDQQRVLV
                     LKFARGL"
BASE COUNT          363 a          835 c          804 g          328 t
ORIGIN      
        1 tggcgcttgg gatcagctta gtcgccggcg tcacggccgg cggggtcgcg gcgggcgacg
       61 ccgcgggcgg gaaactcttc gccggcagca cgccgcgcac cgacggcacg accgtggtct
      121 acgagacgat gggcaacggc aactgggacg tggtcggcgc cagcctcgcc gaccgccagc
      181 agttcccgct cgcgctcggc ccgagccagg cgatgtacgc cgacatcgag ggcgacgtcg
      241 cgatctggca ggagaccaac ggccgcgatg actacgacat cgtcggcaag cacatcaaca
      301 gcaacacgag cttcaccatc gccgccagcg cgaacagcga ggtctacccc gcgctctcgg
      361 atggctgggt ggccttcgtc agcgcgccgc acatcttcgc ggagaccgaa gtgcaggaac
      421 tgcaggtgcg cagcgtggtg acgatggacc ccgcgcgcac gctcgacaag gccgcgctcg
      481 ccggcggccc cagcggcggc ttcctgcgcc ccgccatcag cggcaagcgg gtcgcctggg
      541 tgcgcctgga gcagacttcg ttgcacgtgg tccactggca actgaagact cagcggctgg
      601 atgagacgtc cgctaccgtc atcgccgaag ccgacctcga catcggcggg ccgctcggag
      661 cgctggccat gccgtcggtc gatctctcgg gcgacatcct ggtctacagc gccgacctgc
      721 ggctgaccgt ggtcaatctg gccaccggcg agcgccacga gatcgccgcg gcccaggagg
      781 ccgactatca cccggcgcag aacccgacca ccgacggccg cttcgtcttc tggcaggact
      841 accacggcac cggcgacacc ggcacgatga tcgatatgct ccagcacggc acgctgaccg
      901 ccgccatcgg cggctacgac ctgctgaccg gcagccgctt cacggcggtc gccaaccagg
      961 gctacaacgt cgacccctac gcccgccacg gcctgctgac cttcgcgcgc aaggagtcgg
     1021 tggctcaggg cgagtcgcag gtctacgccc tgccggtcgc cagcctgctg ccgagcgcgc
     1081 cgcagtccga cccgggcgac ccgaacgtct cctactttcc ggagaccggc cactggctgg
     1141 gcggtgagat ccgcgacttc tggaagctct ccggcggact gccggtcttc ggctacccgc
     1201 tgaccgcgga gttcgacgag ggcggcttca cggcgcagta cttcgagcgc cagcgcttcg
     1261 aggtccaccc tgagtaccag ggcacgccct acgcggtcga gctgggcctg ctgggggtag
     1321 aggacgccgc caagcgcggg ctgctcgaca cgacaccctt ccagcggctg tccgaagacg
     1381 cgccgcgcac ggccccttgc gtctacttcg cggccaccgg ccacacggtc tgcgccaact
     1441 tcctcaacta ctggcagagc cacgggctgg agttcggtga tccgggcatc tcctatcgcg
     1501 agtcgctggc gctcttcggc tacccgatca gccagccctt caccgatccg gcgacggggc
     1561 tgacggtgca gtacttcgag cgcgcccgct ttgagtggca ctcggagaac accgcaccct
     1621 acaacctgct gctcggtcgg ctcggcttcg accgtctgag cgagcgcggc tggtagccgc
     1681 gctttgaccc tcacactcgc ctgtagagcg agtgtgaggg tgctgtcagt ggtaccggcg
     1741 cgggcgctgc cgcaggatag ccacacgagt tcagcgcgag tcgggcggcg ggggaaccgg
     1801 cgcgagacgg ccggaggaga gagacggatc atggtccgcg agctgacgcg ggaggagcgg
     1861 atcgagccac tcgagcagcg ctgggcgctg gcgaccggcg aggagttcca ggtcgccgcc
     1921 gccaagcgcg acccggcggc cttcgcgccg ctctacgagc gctacgtcga tgccatctac
     1981 cgctacgcct accgccgcgt cggtagccac gccgaagccg aggacgtgac ggcgcagacc
     2041 ttccagcagg cgctggcggc gctgcccaac tttgaatggc gcggcgtccc cttcggcgcc
     2101 tggctctacc gcatcgccgg ccacgtcatc gtccagcgcg ggcggcggag cagccgcgaa
     2161 ctggtggtgg aggatctgcg cgtccacctc gaccgcggcg accgagcgga cgacgaggat
     2221 ccggcgcggc tcttcgaggc gcgcgccgaa gccggggagc tggtggccgc ggtccggcgc
     2281 ctgccgcgcg atcagcagcg ggtgctggtg ctgaagttcg cccgtgggct
//
LOCUS       DASUBI010000368         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1028025, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000368 DASUBI010000000
VERSION     DASUBI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028025"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..1352)
                     /locus_tag="VFU72_07120"
     CDS             complement(435..1352)
                     /locus_tag="VFU72_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5423292.1"
                     /translation="MTTMLVPLAGVPRDRHAIRFGQTMARALHGTLELLCLSEPIAEE
                     TDPPARSAPVPYLDFTALKDDAELLVDRRIASGHPAEQIAARAAALDDAFVVLALPAA
                     PAAGHRRAWRVAQALLRHCPAPVVLLPARSAAPGSLTSLLVPLDSSPLAQSVLPLVAD
                     IAGRSRAVVELVWAEDLGRLLMRYGVGDETDALEIGRSGEIPQLIDHAARLYLERSAS
                     HLRTQGIDARWQVLTGGAGAAISREAATTAADLIVMATHGRNRLERLAFGSVTQDVLR
                     QARVPVLVLPPAARRRRLSPLVHDVQTVA"
     gene            1738..2205
                     /locus_tag="VFU72_07125"
     CDS             1738..2205
                     /locus_tag="VFU72_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5423293.1"
                     /translation="MSMKQVSLGLLLLRVGSGLALATHGYPKLFGGEGKEAPTLARVL
                     GKNFPAAMERGGAENFSKGLERMGVPFPKHGAYLSALTEFGGGLALALGLATRLVAPA
                     IMVNMLVAIRKAHWSTGFSGQGGYELPSLFTVIAATLCLTGPGRYSLDRLLRG"
     gene            2354..3037
                     /locus_tag="VFU72_07130"
     CDS             2354..3037
                     /locus_tag="VFU72_07130"
                     /inference="COORDINATES: protein motif:HMM:NF018952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HEU5423294.1"
                     /translation="MRTLGVLTIGQSPRADGLTRDVQAIVGPEIRVVERGALDGLSRA
                     EIDRLAPRAEDYHLITMLADGSSVQLGKAAILDRLQARIEQLEAEDDAAATLLMCTGA
                     FPRFNHQRPLFQPQAALYGAVSGLAAGERIGALEPLAVQLDQGHAKWHALGHDDVVLA
                     TANPYLPEPLASIRESAAGVRAAGAGILFMDCFGYSLAMAAAARAAFGGPVVLARSLA
                     ARLAAELIA"
     gene            3161..>3366
                     /locus_tag="VFU72_07135"
     CDS             3161..>3366
                     /locus_tag="VFU72_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423295.1"
                     /translation="MTPPDLLVVVAKYPTPGRVKTRLGQAIGLEQSARLYHAMLLDLE
                     ERLRQLPAHHPHIWAFAPDERDPAA"
BASE COUNT          516 a         1186 c         1176 g          488 t
ORIGIN      
        1 cgccagcgtg agcgtccgca cgccgggcag ctctacggcg gcatcgacga ccgcagcggt
       61 ccaccagcgc aaggaatcga ctggcatggc ctcttcgtat cccgcgctcg tctgctccaa
      121 ctcctccatg actcacctac acctgtatga cgagcgcgtg cccggaggcc aggcgcccaa
      181 cagcggccac acgctcggcg ccggccagga gctgtccggc gaacacgttc ggtgtcgcgt
      241 cagcacggat ccttcgacaa gctcaggatg acagggtgac ggcaggggcc tttcggacga
      301 cacggatcct ctttgtcagc ccgctatgag ccctgtcatc ctgagcccag tcgaaggatc
      361 cgtgctcctc gccagtcgcc ggctcgctcc ccggcaaccc gggctcggca gccggttggg
      421 ccctcggaca gcgcctaggc taccgtctga acatcgtgga ccagcggcga cagccggcgg
      481 cggcgcgcgg cgggcggcag cacgagcacc ggcactcgcg cctgccgcag cacatcctgc
      541 gttacgctgc cgaacgccag ccgctcgagc cggttgcgac catgcgtcgc catcacgatg
      601 agatcggcag cggtcgtggc tgcctcacga ctgatcgccg ctcccgcacc gccggtcagc
      661 acctgccagc gagcgtcgat cccctgcgtt cgcaggtgcg aggcgctgcg ctccagatag
      721 agccgcgccg cgtggtcgat caactgtgga atctcgccgg atcgtccgat ctccagcgcg
      781 tcggtctcat ccccgacccc ataacgcatc aacaggcgcc caagatcctc ggcccagacc
      841 aactcgacca ccgcgcggct gcgcccggcg atatccgcca cgagcggcaa gacggattgc
      901 gcgagaggcg agctgtcgag cggcaccagc agggaggtga gggatcctgg cgccgcgctc
      961 cgcgcgggaa gcaagacgac cggcgccggg cagtgccgca gcaaggcctg cgcgacgcgc
     1021 cacgcgcgac ggtggccggc cgccggagcc gcggggagcg cgaggaccac gaacgcatcg
     1081 tcgagcgcgg cagcccgcgc ggcgatctgc tcggcgggat ggccgctcgc gatccgacga
     1141 tcgacgagga gttccgcgtc atccttgagc gcggtgaaat caagatacgg cacgggggcg
     1201 gagcgtgcgg gggggtcggt ctcctccgcg atcggctccg acaggcagag caactccagc
     1261 gtcccgtgca gcgcgcgcgc catggtttga ccgaatcgga tagcgtgccg atcccgcggc
     1321 acgcccgcga gcggtacgag catcgtggtc atcaagcgcc tcgcttctcc tgccgcgatg
     1381 accgtgcgcg gatccggcgt ctcgtagtcg gttttcccaa gcagatgcgg cagacgctgg
     1441 ccgggcggcg cgtggcggga cgccggacaa cgaacgacac gcacctgatg ccagcgtatg
     1501 cctcgcgatg cgagctgtca ttgcgcggga acaagctcga ccggcagacg ggagacacac
     1561 gcacgccagc ggtcccgccg gcgcctgcag cgcgccggca gccggtcgcc agccgcgccc
     1621 aggctcagcc tacggcggcg tgctccggaa caatgggaaa taatcgcccg tggcgcggag
     1681 tttgaagtgc cggtgcatcg tggaccgagc gacggtacag ggaaggatat ggccggcatg
     1741 agcatgaagc aggtgagtct gggactgttg ctgctgcgcg tgggcagcgg gctggcgctg
     1801 gccacccacg gctaccccaa gcttttcggc ggcgagggca aggaggctcc gacgctggcg
     1861 cgggtgctgg gcaagaactt cccggcagcc atggagcgcg gcggggcgga gaacttcagc
     1921 aaagggctgg agcgcatggg cgtccccttc cccaagcacg gggcgtatct ctcggcgttg
     1981 accgagttcg gtggcgggtt ggcgctggca ctggggctgg cgacccggct ggtggcgcca
     2041 gccatcatgg tcaatatgct ggtggcgatc cgcaaggcgc actggtcgac gggcttctcc
     2101 ggccagggcg gctacgagct gccgagcctc ttcaccgtta tcgccgccac gctctgcctg
     2161 accggtcccg gccgctactc gctcgaccgt ctgctgcgag gctaacgccg cgcggccgcg
     2221 cccggcgcgg tcgtcgcggc gatcagcggc aactgtcttc cgtacgcgct acactgagcg
     2281 gcgacggtgt tccccgatca ccgtgtcgcg cgagctcccg gcgtgcccgg tgatcccaac
     2341 aaggagtcgg aagatgcgta ctctcggcgt cctgaccatc gggcagtcgc cgcgcgctga
     2401 cggtctgacg cgcgatgtcc aggcgatcgt cggcccggag atccgcgtgg tcgagcgcgg
     2461 cgcgctggac ggactcagtc gcgcggagat cgaccgcctg gctcccaggg ccgaagacta
     2521 ccacctgatc acgatgctgg ccgacggaag ctcggtccag ctcggcaagg ccgccattct
     2581 cgaccgcctc caggcgcgca tcgagcagct ggaggcagag gacgatgccg ccgccacact
     2641 cttgatgtgt accggcgcct tcccgcgctt caatcaccag cgacccctct tccagccgca
     2701 ggccgcgctc tacggcgcgg tgtcggggct ggcggccggt gagcgcatcg gcgcgttgga
     2761 gccgctggcg gtccagctcg accagggtca cgccaaatgg cacgcgctgg ggcacgacga
     2821 cgtggtcctg gcgacggcca acccgtacct gcccgagccg ctggcgtcga tccgcgagtc
     2881 cgccgccggc gtgcgcgcgg cgggtgccgg catcctcttc atggattgct tcggctactc
     2941 gctggcgatg gcggccgccg cccgcgccgc cttcggcggt ccggtcgtgc tggcccggtc
     3001 gctggccgcc cgcctggctg cggaattgat cgcgtgaggc gcggtggctg tcccgcgggt
     3061 tgggtggcgc gtgaggacgg atccttcgcc tgcgctcagg atgacaaggg gaaggatgag
     3121 gcctttcgga cgacgcccga cgtgatcgac ggccagcggc gtgacccctc ctgacctgct
     3181 ggtcgtcgtc gccaagtacc cgacgccggg ccgggtgaag acgcgcctgg ggcaggccat
     3241 cggcctggag cagagcgcac ggctctacca tgccatgctg ctcgacctcg aagaacggtt
     3301 gcggcagctt ccggcacacc acccccacat ctgggccttt gcccccgacg agcgcgaccc
     3361 ggccgc
//
LOCUS       DASUBI010000369         5804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1186094, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000369 DASUBI010000000
VERSION     DASUBI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186094"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFU72_07140"
     CDS             complement(<1..762)
                     /locus_tag="VFU72_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5423296.1"
                     /translation="MIERYTRPAMGAIWSERRKIDLWLKVEIAVAEAWSERGVVPPDA
                     LERIRQASCDLDRMKEIEREVDHDVIAFLRATGETVGEAARFIHLGLTSSDVIDTALA
                     MQMVEGLDLLLADVDELLAVVGSEAVKHRDTVMIGRTHGVHAEPITFGFKLAVWYDEL
                     RRGRERLEFARENIRVGKISGAVGTHANVPPEIEEAVCARLGLQPAPVSTQIIQRDRH
                     AQVIEALALAASSLDKFATEIRPLQRTEVRELEEPF"
     gene            complement(772..2103)
                     /gene="mtaB"
                     /locus_tag="VFU72_07145"
     CDS             complement(772..2103)
                     /gene="mtaB"
                     /locus_tag="VFU72_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEU5423297.1"
                     /translation="MTAPRAPRHAPRFAICTLGCKLNQADEDEVRRGLRQAGLVEVAF
                     DDPADIYIVNTCTVTHLADRRSRQMLRRARRQAPESLVAAIGCYPAVSPEELQAMPEV
                     DLVVGSVEKLTVVDDILTRLEWHNAAFDLDPEPSLFSTRTRRMLKIQEGCRAHCTYCV
                     IPTARGKPRNIAPEQIVAQVREAEREGYREVVLTGTHVGTYKWPVPGSLQPLRLADLL
                     EQVLDQTGIERLRVTSVGPHEIDAQFISILQRPRMMPHLHLALQSGSATVLHRMKRWY
                     NLQQFRRAVNQLRAAMPEIGITTDVIVGFPGESDEEFEESCDFVREMAFAKVHVFPFS
                     ARRGTPAAGMPEQVPSRVKEARGARLRALAGEMHAAFVAQQLGREAAVLLEHEAGELQ
                     GRALWSGLSGNYLRVYVPAEPGADVENRIVTVRLRAPYQDGALADAPPAIG"
     gene            complement(2203..3114)
                     /locus_tag="VFU72_07150"
                     /pseudo
     CDS             complement(2203..3114)
                     /locus_tag="VFU72_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(3350..3676)
                     /locus_tag="VFU72_07155"
     CDS             complement(3350..3676)
                     /locus_tag="VFU72_07155"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5423298.1"
                     /translation="MSGIDTALSAYVRAAIKRAQCDWLAESQEYFCAVPELQGAWSTG
                     KSKDEARSELSEVIGDWVQLGLRLHHYIPIVEGIDPNPRSDSENGAHEEPQRSCPRSV
                     QSVEVN"
     gene            4165..>5804
                     /gene="glmS"
                     /locus_tag="VFU72_07160"
     CDS             4165..>5804
                     /gene="glmS"
                     /locus_tag="VFU72_07160"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5423299.1"
                     /translation="MCGIFGFVGGRRPAGDLILGALRTLEYRGYDSWGVAVGVNGQID
                     VAKQAGRIGDAVAVVPAATIGFGHTRWATHGGVTDQNAHPHLDCTGRFAVIHNGIIEN
                     FRTLKNELLARGHEFASQTDTEVVAHLIEEAVSGSEPSDLVTGLRAVFARLRGLNAVI
                     VLDNLTHHLAAVKNVSPLVLGRGPEGYYIASDAVALVEHAGEIHYLNDGEVALLGAEG
                     VQLYDAKSGQERQPNFQPLTITAADTSLHEYPHFMLKEIHEQPRVLRAIGRDYGDYVK
                     ELAALIRASQGTFLIGCGTASYAALSGQYLFSRIARRHVNFVVGSEFKYQEHFINERS
                     LVVALSQSGETADVIESVLAAKARGAKIAALVNVQGSTLDRLADFSVPLGAGPEQCVL
                     STKAYTAKLAILLLTAHEINGSGHAGKEWLWQAIDGVERAISEESESLIRRIAASIVE
                     RDHCFIIGRGLSYPAALEAALKIKEVSYMHAEGFAGGELKHGVIALVEPGTPCIVYSP
                     LDETRDDILSGAMEMKSRGGQIIGVSPVNDEVFDIHVPV"
BASE COUNT         1021 a         1947 c         1831 g         1005 t
ORIGIN      
        1 gaacggctcc tccagctcgc ggacctccgt gcgctgcagg gggcggatct cggtggcgaa
       61 cttgtccagt gagctggccg ccagcgccag cgcctcgatc acctgcgcgt gacggtcgcg
      121 ctggatgatc tgggttgaga ccggggccgg ctgcagcccg agccgcgcgc agaccgcctc
      181 ctcaatctcc ggcggcacgt tggcgtgcgt cccgaccgcg cccgagatct taccgacccg
      241 gatgttctcg cgggcgaact ctagccgctc gcgcccgcgc cgcagctcgt cgtaccagac
      301 ggccagcttg aagccgaagg tgatcggctc ggcgtgcacg ccatgggtgc gcccgatcat
      361 gaccgtatcg cggtgcttga ccgcctcgct gccgaccacc gccagcagct cgtcgacgtc
      421 ggccagcaac agatcgagcc cctcgaccat ctgcatggcc agcgccgtat cgatcacatc
      481 cgaactggtc agaccgaggt ggatgaagcg cgccgcctcg ccgacggttt cgccggtcgc
      541 ccgcaggaag gcgatcacgt cgtggtcgac ctcgcgctcg atctccttca tgcggtcgag
      601 atcgcagctc gcctggcgaa tccgttcgag cgcgtcgggc ggcaccacgc cgcgctcgga
      661 ccaggcctcg gccaccgcga tctccacctt gagccagaga tcgatcttac gccgctcgct
      721 ccagatcgcg cccatcgccg gtcgggtata gcgctcgatc acaccgctct cctagccgat
      781 cgccggcggc gcatccgcca gcgctccgtc ctgatacggt gcacgcagcc gcaccgtcac
      841 gatgcgattc tcgacgtccg cccccggctc ggccggcacg tagacccgca ggtagttccc
      901 gctgagcccg gaccagagcg cccgtccttg cagttcgccc gcttcgtgct caagcagcac
      961 cgcggcctcg cgcccaagct gttgagcaac gaatgcggcg tgcatctcgc ccgccagcgc
     1021 gcgcagccgc gcgccgcgcg cctctttgac cctggacggc acctgctcag gcattccggc
     1081 cgccggtgta ccgcgccgcg ctgagaaggg gaagacgtgc actttggcga aggccatctc
     1141 acgcacgaag tcgcaggact cttcgaactc ctcgtcgctc tcgcctggga agccgacgat
     1201 cacgtcggtg gtgatgccga tctcgggcat ggccgcgcgc aactggttca ccgcgcgacg
     1261 gaactgctgc aggttgtacc agcgcttcat gcggtgcagc acggtcgcgc tgccgctctg
     1321 cagggccagg tgcaggtgcg gcatcatgcg cgggcgctgc aggatgctga tgaattgggc
     1381 gtcgatctcg tgcggaccga ctgaggtcac gcgcaggcgt tcgatgccgg tctggtcgag
     1441 cacctgctcc agcagatcgg ccaaacgcag cggttgcagg ctccccggca cgggccactt
     1501 gtaggtgccc acgtgcgtgc cggtcagcac gacctcgcgg tagccttcgc gctcggcctc
     1561 gcgcacctgc gccacgatct gctcgggcgc gatgttgcgc ggctttcccc gcgccgtggg
     1621 gatgacgcag tacgtacagt gggcgcggca gccctcctgg atcttcagca tgcgccgcgt
     1681 gcgcgtcgag aagagagatg gctcgggatc caggtcgaag gcggcattgt gccactcgag
     1741 ccgggtcagg atgtcgtcga ccacggtgag cttctcgacc gagccgacca ccagatcgac
     1801 ttccggcatc gcctgcagct cctcgggcga gaccgccgga tagcagccga tcgccgccac
     1861 cagcgactcc ggcgcctgac ggcgggcgcg gcgcagcatc tggcgcgagc ggcggtcggc
     1921 caggtgggtc acggtgcaag tgttgacgat gtagatgtcg gccggatcgt cgaaggcgac
     1981 ctcgaccagg cccgcctggc gcagcccgcg ccggacctcg tcttcgtcgg cctgattgag
     2041 cttgcaaccc agggtacaga tcgcaaagcg cggcgcgtgc cgcggcgctc ggggcgctgt
     2101 caagaggtga atcggagtgt ccatcttccc caacgcgcga acagcgaatc tacctctgga
     2161 ttataccgaa ataccgctgg ccgcgttcgg acggggactt ggtcactcca ttcgatgccc
     2221 gccaccgacg aagaaaagtt ggccggtcag ccagcgcgcc tgctcagagg cgaggaagac
     2281 gatgacgtcg gctacgtctt cgggttgccc aatccgaccg agcggaatct cgcggacagc
     2341 gatcgcctcg tgctcgggcg tgatccagcc tgtctggatc ggccccggcg acacgatatt
     2401 gacggtgatc ccatactgcc ccagctcacg cgcggcggca cggctgtagg actccatcgc
     2461 gtacttgctg gcgccgtacg acacttcgtt ggggaagttc gatgccccat cggtgctgac
     2521 gttgatgatc cgcccccaag acgcgcccct ctaaacgaga cgtcgcgcga actccgccat
     2581 gaggagagcc acggcgcgcg tattcaccgc gaaatgctga ttgatcgttc gtggatcaat
     2641 tctcggaatc ggttccctgg tccacaaatg gggcaacgga ttggcgagat cggtcaggcg
     2701 aggcacgaag gtatcgggcc ccgcagcggc ggcgttgttg acgaggatat ctactggccc
     2761 aaattccgcc tgcgccccgt ccaaaaggcg agcaatattt tccggattgg cgaggtcgat
     2821 ttcgagcgac gctgcctccc cgccctcctg ctggatcgcc gcgacaacgt catcagcggt
     2881 cgcggctcgg ttctcgtgat agaggcgctc atcggatagt aatgcgcccg gaggaggtgg
     2941 ctgccgcaga tactgaatga agatcttgac gccttgcgct gccagcgcct cagcagttgc
     3001 cgcaccgatt ccgtgattgc cacccgtaac gagggcatgt tttccggtaa gacctggatc
     3061 aagcatcgct cttctccgac ggaggcactt tggaggcgga taggacgcag tcatggaact
     3121 gttatgaccg ggtagtactt actaataata ccaccattaa agtgattccc attcagttcg
     3181 agatagccct gcttgacgga gaattctgct tagggttcga cgccgacatc gccttggtgc
     3241 gggttcggga tgcgcaggcg tacttgtccc tgaatcatga aatcgtgctt ggcccccgga
     3301 aaatggccca tcaaatccgg cgcggtgtag aaatcgaatc aattcacgtc taattcacct
     3361 ctactgattg gaccgaacgg gggcacgagc gttgtggctc ctcgtgagca ccgttttcgc
     3421 tgtcgctccg aggatttgga tcgattcctt cgacgatcgg aatgtagtga tgcagcctca
     3481 atccgagttg aacccagtcc ccgataacct cactcagttc acttcgtgcc tcgtctttgg
     3541 acttgcccgt cgaccaagca ccctgtaatt caggaactgc gcaaaaatac tcctggcttt
     3601 ccgccagcca atcacactga gcacgcttga tcgccgcacg aacataagca gacaacgctg
     3661 tatcgatccc gctcatcaca cggttcccac tccaacagca gaagtcgcaa tcgaccgcac
     3721 tatcctcgcc aattatccac caggatgagt cttctcggcg gcaaagcgcg gagtattgcc
     3781 gtcagacgaa cggcccttgt cactggcact ccgaaggact atagtgaccg cagcaagcgc
     3841 ccgcggagga agcgggagcg cacgtacgcg ggagagacga gcgaacggac agcaaccagt
     3901 agcgccggga cttgccgtgg aggcggcgag tcgacgaggt gaaggccgat cgaaagcttc
     3961 ggcggatggc cttccggccg gcacggtcga gacggtcgcg cgaaatccgg tgagcaatcg
     4021 ccgggacaag cggcggccgg tgtgcggaca gattaccctc aaccttctcc cgtgaacaac
     4081 gtgccttcct gtccgttggg gcgatgcggt gcgcggccac gatggcggcc tgcgctcgac
     4141 tcaacatgga aggaatgtgc cgggatgtgt ggcatcttcg gcttcgtcgg cggccgtcgt
     4201 ccggccggcg acttgatact cggcgccctg cgcaccctcg agtaccgtgg ctatgactcc
     4261 tggggtgtcg cggtcggcgt caatggccag atcgacgtcg ccaagcaggc cggtcgcatc
     4321 ggcgacgcgg tcgcagtcgt gccggcggcc accatcggct tcggccacac ccgctgggcg
     4381 actcacggcg gcgtcaccga ccagaacgcg catccgcatc tcgactgcac cggccgcttc
     4441 gcggtcattc acaatggcat catcgagaac ttccgcacgc tcaagaatga gctgctggcg
     4501 cgcgggcacg agttcgccag tcagaccgac accgaggtgg tggcgcacct catcgaggaa
     4561 gcggtcagcg gcagcgagcc atccgacctg gtcaccggtc tgcgtgcggt cttcgcccgg
     4621 ctgcgcggcc tcaacgccgt catcgtcctc gacaacctga cccaccacct ggcggcggtc
     4681 aagaacgtct caccgctcgt gctcgggcgc ggcccggagg gctactacat cgcctccgac
     4741 gcggtggcgc tggtcgagca cgccggcgag attcactacc tcaacgacgg cgaggtcgcg
     4801 ctgcttggcg ccgaaggcgt gcaactctac gacgcgaagt cgggccagga acgccagccg
     4861 aacttccagc cgctgacgat caccgccgcc gacacctcgc tgcacgaata cccccacttc
     4921 atgctcaagg aaatccacga gcagccccgg gtgctgcgcg ccatcgggcg cgactatggc
     4981 gattatgtca aggaattggc cgcgctcatc cgtgcgtcgc agggtacctt cttgatcggt
     5041 tgcggcacgg ccagctacgc cgcgctctcc ggccagtatc tcttctcgcg catcgcccgc
     5101 cgccacgtga acttcgtggt cggctccgag ttcaagtacc aggagcactt catcaacgag
     5161 cgcagcctgg tggtggccct ctcgcagagc ggcgagaccg ccgacgtaat cgagtcagtg
     5221 ctggcggcca aggcgcgcgg cgccaagatc gccgcgctgg tcaacgtgca gggctcgacg
     5281 ctcgaccggc tggccgactt cagcgtgccg ctcggcgccg gcccggagca atgcgtcctc
     5341 tcgaccaagg cctacactgc caagctggcc atcctgctcc tgacggcgca tgagatcaac
     5401 ggcagcggcc acgccggcaa agagtggctc tggcaggcga tcgacggcgt cgaacgcgcc
     5461 atcagcgagg agtcggagag ccttatccgt cggatcgcgg ccagcatcgt cgaacgcgat
     5521 cactgcttca tcatcgggcg tggtctctcc tacccggccg cgctggaggc ggcgctgaag
     5581 atcaaagagg tgagctacat gcacgccgag ggcttcgccg gcggcgagct gaagcacggc
     5641 gtgatcgcgc tggtcgagcc gggcacgccc tgcatcgtct attcgccgtt ggacgagacc
     5701 cgcgacgaca tcctctccgg cgcgatggag atgaagagcc gcggcggcca gatcatcggc
     5761 gtctcgccgg tcaatgacga ggtcttcgac atccacgtgc cggt
//
LOCUS       DASUBI010000370         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_273115, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000370 DASUBI010000000
VERSION     DASUBI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273115"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFU72_07165"
     CDS             <1..945
                     /locus_tag="VFU72_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEU5423300.1"
                     /translation="LADVSVEAPDGSLTALLGPSGSGKSTLLRVIAGLETPDAGEVTI
                     DGTAATGLSPQRRNVGFVFQHYAAFKHMTVRANIGFGLRIRNRPKPEIARRVDELLAL
                     VQLDGFGDRYPSQLSGGQRQRMALARALAVEPRVLLLDEPFGALDARVREELREWLRR
                     LHEQVHVTTVLVTHDQSEAMDVASRIVVMNHGRIEQVGTPAEIYERPATEFVMTFVGK
                     VNRLPDRFIRPHDVAIRPAGVSGCEPATVEQIRSLGFDAWVALRLASGETLWAQLTRA
                     ELDRLHLAPGMTVGADLTQRVRFVAKDDRDVPELARAR"
     gene            2666..3127
                     /locus_tag="VFU72_07170"
     CDS             2666..3127
                     /locus_tag="VFU72_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423301.1"
                     /translation="MPRRGLGGGLRQHVAAAVTGGVESLRQGAVDFEATAVDGSIVPQ
                     EGVFASASKDWDPQKAPLVWANMGEPIIYEIAIAGDLGVATYADLEGERVAWRADFPE
                     VNLNTRVYLAYGGLTWGDMERVAVHGFFDATLKALENGDLDAAFVWRLTQR"
     gene            3265..>5565
                     /locus_tag="VFU72_07175"
     CDS             3265..>5565
                     /locus_tag="VFU72_07175"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423302.1"
                     /translation="MPQLPTGTVTFLFTDIEGSTALWERDRAAMREAVARHLVILNRI
                     IEEHHGVLYKIVGDGTQSAFASAEDAVRAAVAAQRAFQQEAWADPPGVLRVRMALHAG
                     EAAPDARGDYLAAPLNRLSRLLAIGHGGQILLTQTVQQLCRDGLPVDVALRDLGEHRL
                     RDLREPERVFQVLHPDLPAVFPPLKSLDARPTNLPRQPTAFIGREREVAEVVAQLRQP
                     GTQLVTLTGPGGAGKTRLALQAAAELLDDFPDGVWFVPLASLRDPALVLPAIATAVGV
                     RDEAGQPLGERLRDYFAGRRALLVLDNVEHLLAAAPLVAELLAAAPGLTVAATSRAPL
                     RLQAEREYPVASLPLPRRTPPPSPEHLAHYDAVRLFVDRARAVKPTFTVDDANAPAVA
                     EICHRLDGLPLAIELAAARVRMLPPEALLRRLDQRLPLLTGGTRDAPARQRTLRDTIA
                     WSHDLLRPDDQILFRGLAVFAGGATFAAIEAVVEDEEIDCWNGLEQLLEHNLVRQADG
                     PEREPRFVMLETIREYGWERLAEAGELERMRRAHAAYFLALAEEAEPELKGPRQGTWL
                     QRLETDHDNLRAALDWSTASQEPELGLRLAAALSRVWSIHGHLTEGREWLERALATRG
                     AAAPATRARALNGAGFLTHMQGDYPQAVALYEEALTLERELGDKGGMATSLNNLGIVA
                     LEQGDYPRATALYEEALTLQRALGNKDSAANSLNNLGNVACEQGDYSRATALYEEALA
                     LDRALGNQRGIARSLNNLGSVAKQQGD"
BASE COUNT          868 a         1894 c         1981 g          822 t
ORIGIN      
        1 ctcgccgacg tttcggtcga agcgccggac ggatcgctga cggccttgct cgggccgagc
       61 ggcagcggca agagcacctt gctgcgggtg atcgccgggt tggagacgcc cgacgccggc
      121 gaggtgacga tcgacggcac agcggcgacg ggattgtcgc cgcagcgacg caacgtcggc
      181 ttcgtctttc agcattacgc ggcgttcaag cacatgaccg tccgcgccaa catcggcttc
      241 ggcttgcgga ttcgcaaccg gccgaagccg gagatcgcgc ggcgggtcga cgaattgctg
      301 gcgctggtgc agttggacgg cttcggcgac cgctatccgt cgcaactctc gggcgggcag
      361 cggcagcgga tggcgctggc gcgggcgctg gcggtcgagc cgcgggtgtt gctgctcgac
      421 gagccgtttg gcgcgctcga cgcccgcgtc cgcgaagaat tgcgggaatg gctgcgccgg
      481 ctgcacgagc aggtccacgt gacgacggtg ctggtgacgc acgaccaaag cgaagcgatg
      541 gacgtggcct cccggatcgt ggtgatgaac catggccgga tcgagcaggt cggcacgccg
      601 gccgagattt acgagcggcc ggcgaccgaa ttcgtgatga ccttcgtcgg caaggtcaac
      661 cggctgcccg accgcttcat ccggccgcac gacgtcgcga tccgtcccgc cggcgtttcg
      721 ggctgcgagc cggcgacggt cgagcagatt cgctcgctcg gcttcgacgc ctgggtcgca
      781 ttgcggctcg cctcgggcga aacgctctgg gcgcaactca cgcgggccga actcgaccgc
      841 ctgcatctcg cgcccggcat gaccgtcggc gccgatttga cgcaacgggt gcggttcgtc
      901 gccaaggatg accgcgacgt tcccgaactg gcccgcgcca ggtaggcaac cgacccccgt
      961 gatttcttgc attggatgga aacgagccgg cgccctgccg agcatgaact ccgccgcgct
     1021 ggttccgaga atgaggcagc gagttgaaaa aagggtcgtg gtggtcgaca ggggcgacga
     1081 tacggcgcac cttgctccgg tttgcctaag tttgatccgg ctcgcggtcg cgccgaatga
     1141 tctcccagac ctgattgctc ccaccgagcg agtttttcaa catgctggat cgtgtgaccc
     1201 gcgggatggc gcgctcccgg ggagatcact ccgaaccgcg ccaggtcaag ccgttggggg
     1261 cgccgcgggc ggcgtcgcta gcgcccctgc cagcgccggc gtttggcgtc gccggccgtg
     1321 ccgccggcca ccgtggcgac gcatccagcg ccagcgcgca gactggagga cgaagtcctg
     1381 atccgtatcc ggtcaggctg atggcgggtc tatcccgcga atggccgcga tcgacaagat
     1441 gatcggcgcg tcggcgacgg ttctgttctg caaacacggt ttggcagaag ccccatcccc
     1501 gggttcgaac ccagagatgg gcaggggttc atccaagccc ccgcggttga gcccggatga
     1561 cgcatggagg ccgagtgcag ccggctggcg ctccggcgca cgccgtccgc gagcctggcg
     1621 ccgcgcggga ggcgttcgct gcagctcatc ggcacgagaa gtttcccgaa ctatgcagtt
     1681 ggcagatctc caaacaacac accgggccac cctgggtcaa acagcaggtg ggacgggggc
     1741 aacacatcaa gcctcccccg cctccctccg gcaagccgca gctgcgcggg tcgcaacatg
     1801 ccgccgtctg gccagcgccg caacaaacct gccccgtcgg gcaccccccg cagtcgtcct
     1861 cctgaccgtc cggacagcag gcctgaccgc agaggccggg atgccccgac ggggcggccg
     1921 gaccacaggt gaaacacaac cctttgccat gggcggcgtc gctgatgcat tggccgcgtt
     1981 gcggtcccgg cgggaagacg gtcgtgcaga aatgggcgca attgcgagcg ctcgccttcg
     2041 ccttgttggc tgcgccatgt cgacggtggc ggcgtttcgc ctcactcgtg ccggcgacgc
     2101 cggcgagggc cgcgccgagc agcccgccca gcgcccgacg ccgggaggcg gattgactga
     2161 aggtgcgagc cacccgatca aaggcgtggg ggtccatgcg cccggctcct cccacgtgga
     2221 atgtagcgtc ggtcgggtca acgtggtcaa ccgcggcgag tgtggcggga gccgccgtga
     2281 ttgtcaatca gcagaccagg aaatcggcat gacgatttca tcgacgccag gcgggtgggc
     2341 cgcggcggtg gtcaccctga tcgactggct ccgcagcctg ttgaagaact cccggggtat
     2401 cgcgtccgag gctttttggg tcgctcgtcg accccgaccc cgatgttcgg cgcgcagggt
     2461 cccacgaacg ccgcggcccc ctcacctgct ccgatgggca aaggacgatc cgacgtagcg
     2521 tgcctggctg agggtcaaac gggagcgccg cgcaagcggc ttcccgcctg gagcgccgcc
     2581 aacgaggggc gccgcctggc cgagcgaccg gagcgacagg aaggctccgc cccgatacgt
     2641 caggaggcaa tcatggaacg tctcgttgcc gcggcgtgga ctcggcggcg gcttgcggca
     2701 gcacgtggcg gccgccgtca cgggcggcgt cgagtcgctc cggcagggag cggtcgattt
     2761 cgaggcgacc gcggtggacg gatcgatcgt gccgcaggag ggtgtgttcg cgtccgccag
     2821 caaggattgg gacccgcaaa aggcgccact cgtttgggca aacatgggcg agccgatcat
     2881 ctatgagatc gccatcgcgg gggatctcgg cgttgcgacc tatgccgatc tcgagggcga
     2941 gcgcgtcgcc tggcgcgccg atttcccgga ggtcaacctg aacacccggg tctacctggc
     3001 ctacggcggc ctcacctggg gcgacatgga gcgagtagcc gtccacggct tcttcgacgc
     3061 caccctgaag gcgctggaga acggcgacct ggacgccgcc ttcgtctgga gactcaccca
     3121 gcgatgagca cgggatcggc gccgaaccga ggcattggag ccgccgggcg ctggtccggg
     3181 acgagcggcc cgttcccgcc tgccccctcc cgcatgcgat catgccgtcc accaggagcc
     3241 aaggccagtc ctcggagcgc gatgatgccc cagcttccca ccggcacggt cacctttctc
     3301 ttcaccgaca tcgagggcag caccgcgttg tgggagcggg atcgtgccgc gatgcgggag
     3361 gcggtcgcga ggcacctggt catcctcaac cgcatcatcg aggagcacca cggagtcctc
     3421 tacaaaatcg ttggcgatgg aacccaatcc gcctttgcct ccgccgaaga cgccgttcgc
     3481 gccgcggtcg cggcgcaacg ggcgttccag caggaggcgt gggccgatcc tcccggcgtg
     3541 ctgcgggtgc gcatggcgtt gcatgctgga gaagcggcgc ctgatgcgcg gggggactac
     3601 ctggccgcgc ccctcaatcg gctctcccgg ctcctggcca tcgggcacgg cggccagatc
     3661 ctcctgaccc aaacggtgca gcagctctgt cgcgacgggt tgccggttga tgtcgcgctg
     3721 cgcgacctgg gggagcatcg gctccgtgac ctgcgcgaac cagagcgcgt cttccaggtg
     3781 ctgcaccccg acctgcccgc cgtctttccg cccctcaaga gcctcgacgc ccgccccacc
     3841 aacctgccac gccagccgac ggcctttatc gggcgcgagc gcgaggtggc cgaggtcgtg
     3901 gcgcagctgc gacaacccgg gacgcagctc gtcaccttga ccggccccgg cggcgccggg
     3961 aagacgcggc tggccctcca ggccgccgcc gaactgctgg acgactttcc ggacggggtc
     4021 tggttcgtgc cgctggcgtc gttgcgggac ccggccctgg tcttgccggc catcgccacg
     4081 gcggtggggg tgcgggacga ggcggggcag ccgctcggag agcggctgcg cgattacttc
     4141 gccggccggc gggcgctgct cgtgctggac aacgtcgagc acctgctcgc ggccgcgccc
     4201 ctggtcgccg agctcctcgc cgcggcgcca ggcctgaccg tggcggccac cagccgcgcg
     4261 ccgttgcggc tccaggcgga gcgggagtat cccgtcgcat ccttgcccct gccgcggcgg
     4321 acgccgccac cctctccgga gcacctcgcc cattacgacg ccgtccgcct cttcgtcgat
     4381 cgcgcccggg cggtcaaacc gacgttcacg gtcgacgacg cgaacgctcc cgccgtggcc
     4441 gaaatctgcc atcgtctcga cggcctgccg ctggccatcg agctggcggc ggcgcgggtg
     4501 cggatgctgc cgccggaggc gctcctgcgg cggctggacc agcgactgcc gttgctgacg
     4561 ggcggaaccc gggacgctcc ggcccggcag cgaaccctgc gcgacaccat cgcctggagc
     4621 cacgacctgc tccgtccgga cgaccagatc ctcttccgcg gtctggcggt cttcgcgggc
     4681 ggcgcgacct tcgcggcaat cgaggcggtg gtcgaagacg aggagatcga ctgctggaat
     4741 gggctggagc aactcctcga gcacaacctg gtccggcaag cggacggacc cgagcgcgag
     4801 ccccgctttg tgatgctgga gacgatccgg gagtacggct gggagcggct tgcggaggcc
     4861 ggggagctgg agcgtatgcg gcgggctcac gccgcatact tcctcgccct ggccgaggag
     4921 gccgagcccg agctcaaggg gccacggcag gggacgtggc tgcagcgcct ggagaccgac
     4981 cacgacaacc tgcgggcggc cctggactgg tccacggcga gccaggagcc ggagttgggt
     5041 ctccggctcg cggcggccct gtcgcgggtc tggtccatcc acggccatct gacggaggga
     5101 cgggagtggc tggagcgggc gctggccacg cgtggagcag ccgcgccggc gacacgggcc
     5161 agggcgttga acggagccgg cttcctgacc catatgcagg gtgactaccc gcaggcagta
     5221 gcgttgtacg aggaagccct gacgctggag cgagagctcg gggacaaggg cggcatggcc
     5281 acctccctca acaacctggg gatcgtggcg cttgagcaag gcgactaccc gcgggcgacg
     5341 gcgttgtatg aggaagccct gacgctgcag cgggcgctgg ggaacaagga cagcgccgcg
     5401 aactccctca acaacctcgg gaacgtggcg tgcgagcagg gcgactactc gcgggcgacg
     5461 gcgttgtatg aagaagccct ggcgttggac cgggcgctag ggaatcagcg cggcatcgcc
     5521 agatctctca acaacctcgg gagcgtggcg aaacagcagg gcgac
//
LOCUS       DASUBI010000371         6281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_832143, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000371 DASUBI010000000
VERSION     DASUBI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832143"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /gene="carB"
                     /locus_tag="VFU72_07180"
     CDS             complement(<1..1819)
                     /gene="carB"
                     /locus_tag="VFU72_07180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5423303.1"
                     /translation="MSAGDTQPVQSVLVIGSGPIIIGQAAEFDYAGSQAIRALREEGV
                     RSILVNSNPATIMTDEDLADVVYIEPLTVESLRRIIAQERPDGLLPTLGGQTGLNLAV
                     RLAEAGVLEEFDVRLLGTPLEAIKRAEDRELFKELLIEIGEPVIESAIVHTLPEAHAF
                     VEQVPLPLIIRPAYTLGGTGGGIATTPEDLDRLVASGLNASPIHQILLERCLTGWKEI
                     EYEVMRDANDTCITICNMENLDPMGVHTGDSIVVAPSQTLSDREYQMLRSASLRIIRA
                     LGIEGGCNIQFGLDPKSFQYYVIEVNPRVSRSSALASKATGYPIARVAAKIAVGKRLD
                     EVLNSVTQQTTAAFEPALDYVVVKIPRWPFDKFTRADRTIDTQMKATGEVMAIDRSFE
                     AALQKAVRSLESDQNDLLWEDPVWNVSGEDLWEHIRRPNDLRLWAMSAALRRGAAPEE
                     VSAASGVDPWFTRKLKRLIRLEERITHEPLTPELLWQAKRAGFGDAQIADLTGRDAAA
                     VRALRYETGIVPVYKMVDTCAAEFEAMTPYFYSTYEEEDEAPPLPGRKALVVGSGPIR
                     IGQGIEFDYASVKAAQTLREVGWGAIMVNSNPETVSTDFD"
     gene            complement(1822..2979)
                     /gene="carA"
                     /locus_tag="VFU72_07185"
     CDS             complement(1822..2979)
                     /gene="carA"
                     /locus_tag="VFU72_07185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5423304.1"
                     /translation="MTKLHLTGALVLEDGRIFRGEPFGARGDAEGEVVFTTAMTGYQE
                     VATDPSFYGQIVCMTYPLIGNYGVTPGDEESRQPWIAGMVVREYCDEPENWRSEGGFA
                     EYLQRHAIPALHSVDTRALTRHIRAHGDMRGLLVSDAAGRSDDELRARARAAWTPAEA
                     NVVEAVTTPSEREFAAGEPLHLVLMDCGVKENIMRSLERRNIRVTVVPYDTPASAILK
                     LRPDGVLTSPGPGDPRHTAGAVDTVSAILDAGLPYMGICLGHQLLGLAIGASTSKLKF
                     GHRGANHPVKDLRTGAVHITSQNHGYQVDAGSVPAGAGWHVSQVNLNDGSVEGLAHET
                     LPVFSIQYHPEGSPGPQDNQYLFDRFIEQVRRHQAATNPQFAVATAAGDQE"
     gene            complement(2972..4279)
                     /locus_tag="VFU72_07190"
     CDS             complement(2972..4279)
                     /locus_tag="VFU72_07190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5423305.1"
                     /translation="MFRGGRIIDPEQGLDERADLLVQDGRIAAIGRGLTQPEGAEEFD
                     AANLLVMPGLVDVHAHLRDPGFTAKETLETGAQSALQGGFTTVCCMPNTNPALDTPER
                     VADIVARARELPLRLFPLGAISLGRRGEELADLAGMTEAGAVGFSDDGDSTRSSQVMR
                     EALAWSVASGKPIMVHCEDWTLINGGVMHEGAVSRELGLPGIPAAAEEIITSRDIELA
                     RLTGGWLHVLHVSTELGRDLVRRAKEQGVHVTAEVMPHHLLLTDEWVAGRRRFVGDEC
                     VYATVRPDPNAKVNPPLRTEPDARALLAGLREGTFDILATDHAPHAPEDKPGDLRRAA
                     SGMIGFELALPLLLRLVRAGDLSLLTLVDLLSTRPARLFGLPGGRLRPGSPADLIVVD
                     PEQEWNVNETTIASKSKNSPLLGMTVQGKVRLTMVNGEVRYRD"
     gene            complement(4577..5410)
                     /locus_tag="VFU72_07195"
     CDS             complement(4577..5410)
                     /locus_tag="VFU72_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5423306.1"
                     /translation="MTGSRPGDREDNHCRSTHRARARLRSRYVWIPILYLAAYVIFVR
                     LRAFANQTGIPVRFRYPIQVDHALFAGHDPTVWLQATLRQAGHINWLDSLLVAVYVTY
                     FFVPHLVALYFWLRRDVRTVRYVLAFFGTLYVGLIPIFLVPTAPPWLASERGYLPPFM
                     RVVPTAINHIIPGSFGIGDQLAGQNDVAAMPSLHMAGICVVAFVLARWRAPLGAILAV
                     VYILAMGFALVYLGEHYLSDVVAGLVLAAAVWYVAQRVARHRGGGLSRSTSTERQARS
                     G"
     gene            complement(5513..>6281)
                     /locus_tag="VFU72_07200"
     CDS             complement(5513..>6281)
                     /locus_tag="VFU72_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU5423307.1"
                     /translation="LAIVCAMKTELRHAMEATEITGERRIGPWTITEGSIERRAVRLV
                     RSGIGMVNAGAALSALLAVERPAAVLNYGCAGAHRADLLPGDVVIGERVVAPQSLTIL
                     PGGEERYTGLFCALGAEDKPVEEIDADPGLLAGARHAAEGWQPERWPGSPRGSRPPVV
                     ERGVVASADCWTQHAPRIEALHGRHGSLCEDMEAAALAQVCHLWSVPFLTVKDISNNE
                     LLAVTELGDDLPTLEPHLDEIGRRAWALLRRTLPLIP"
BASE COUNT          976 a         2182 c         2146 g          977 t
ORIGIN      
        1 tgtcgaagtc ggtcgaaacg gtctcggggt tgctgttgac catgatcgcg ccccagccga
       61 cctcgcgcag cgtctgggcg gccttgacgc tggcgtagtc aaactcgatt ccctgaccga
      121 tgcggattgg gccggagccg accaccagcg ccttgcgacc cggcagcggc ggcgcctcgt
      181 cctcttcctc gtaggtgctg tagaagtagg gcgtcatggc ctcgaactcg gcggcgcagg
      241 tgtcgaccat tttgtagacc ggcacgatcc cggtctcgta gcgcagcgcg cgcacggcgg
      301 cggcgtcgcg cccggtcagg tcggcgatct gggcgtcgcc gaagccggcg cgtttggcct
      361 gccagagcag ctcgggcgtc agcggctcgt gcgtgatgcg ctcctccagc cggatgagcc
      421 gcttcagctt gcgcgtgaac cagggatcaa ccccgctggc cgcgctgacc tcttcgggcg
      481 cggcgccccg ccgcagcgcg gcgctcatgg cccagaggcg caggtcgttg gggcgacgga
      541 tgtgttccca gagatcctcg cccgagacgt tccagaccgg gtcctcccag aggagatcgt
      601 tctggtccga ctccagcgag cgcaccgcct tctgcagcgc cgcctcgaag gagcggtcga
      661 tggccatcac ctcgccggtg gccttcatct gcgtatcgat ggtgcggtcg gcgcgggtga
      721 acttgtcgaa gggccagcgc ggaatcttga ccaccacgta gtcgagcgcc ggctcgaaag
      781 ccgccgtcgt ctgctgcgtg accgagttga gcacctcgtc gagccgcttg ccgaccgcga
      841 tcttggccgc cacgcgggcg atcgggtagc cggtcgcctt cgaggccagc gccgaggagc
      901 ggctgacgcg cgggttgacc tcgatcacgt agtactggaa cgacttggga tcgaggccga
      961 actggatgtt gcagcccccc tcgatgccca gcgcccggat gatgcgcagc gaggccgagc
     1021 gcagcatctg gtactcacga tcgctcagcg tctggctcgg cgcgaccacg atgctgtcgc
     1081 cggtgtggac ccccatcggg tccaggttct ccatgttgca gatagtgatg caggtgtcgt
     1141 tggcatcccg catcacctca tactcgatct ctttccaacc ggtcaggcag cgctccagca
     1201 ggatctggtg gatcgggctg gcgttcaggc cgctggcaac caggcggtcg agatcctcgg
     1261 gtgtggtcgc gatcccgccg ccggtgccgc cgagcgtgta ggccgggcgg atgatcagcg
     1321 gcagcggcac ctgctcgacg aaggcgtgcg cctcgggcag cgtatgcacg atggcgctct
     1381 cgatgaccgg ttcgccgatc tcgatcagca gctccttgaa cagctcgcgg tcctcggcgc
     1441 gcttgatcgc ctccagcggt gtgccgagca gccgcacgtc gaactcctcc agcacgccgg
     1501 cctcggccag ccgcacggcc agattgagcc cggtctggcc gcccagcgtc ggcagcaggc
     1561 cgtccggccg ctcctgggcg atgatgcgcc gcagcgactc gaccgtcagc ggctcgatgt
     1621 agaccacgtc ggccaggtcc tcgtcggtca tgatcgtagc cgggttggag ttgaccagga
     1681 tcgagcgcac gccctcctcg cgcagggcgc ggatggcttg cgagccggcg taatcgaact
     1741 ccgcggcctg gccgatgatg atcggccccg agccgatgac caggacactt tggaccggtt
     1801 gggtgtctcc tgcgctcatg tctactcctg gtctccggcc gccgtggcga cggcgaactg
     1861 cgggttggtc gccgcctggt gacggcgcac ttgctcgatg aagcggtcga agaggtactg
     1921 gttgtcctgc ggccccggcg agccttcggg gtgatactgg atcgagaaga ccggcagcgt
     1981 ctcgtgggcc agcccctcga ccgagccgtc gttgagattg acctgcgaca cgtgccagcc
     2041 ggcgcccgcc gggacgctgc ccgcgtccac ctggtagccg tggttctgcg aagtgatgtg
     2101 aacagcgccg gtgcgcaggt cctttaccgg gtgattggcg ccgcggtgtc cgaacttcag
     2161 cttgctggtg ctggcgccga tcgccaggcc gagcaactgg tggcccaggc agatccccat
     2221 gtagggcagc ccggcgtcga ggatcgcgct cacggtgtca accgcaccgg ccgtgtgccg
     2281 cgggtcgccc ggccccggcg aggtgagcac gccatccggg cgcagcttca ggatcgcgct
     2341 cgccggggtg tcgtagggca cgaccgtcac gcggatgttg cgccgctcca gcgaacgcat
     2401 gatgttctcc ttgacgccac agtccatcag caccaggtgc agcggctcgc cagcggcgaa
     2461 ctcgcgctcg ctcggcgtcg tcaccgcctc gaccacgttg gcctcggccg gcgtccaggc
     2521 ggctcgcgcc cgcgcccgca gctcgtcgtc ggagcgtccg gcggcgtcgg agaccagcag
     2581 gccgcgcatg tcgccgtgcg cgcggatgtg ccgcgtcagg gcgcgcgtat cgaccgagtg
     2641 cagcgccgga atggcgtgtc gctggagata ctcggcgaaa ccgccctccg agcgccagtt
     2701 ctcgggctcg tcgcagtact cgcgcaccac catgcccgcg atccagggct ggcgcgactc
     2761 ctcgtcgccg ggcgtgacgc cgtagttgcc gatcagcgga taggtcatgc agacgatctg
     2821 gccgtagaat gacgggtcgg tggcgacctc ctggtagccg gtcatggcgg tggtgaagac
     2881 cacctcgccc tcggcgtcgc cgcgcgcgcc gaacggctcc ccccgaaaga tccgcccgtc
     2941 ttcgaggacc agcgcccccg tcaaatgcag cttagtcacg gtagcggacc tccccattca
     3001 ccatcgtcag tcgcaccttc ccctgcaccg tcatccccag cagcgggctg ttcttgctct
     3061 tggaagcgat cgtcgtctcg ttcacgttcc actcctgctc cggatcaacc acgatcaggt
     3121 cggcgggcga gcccggcctg agccggccgc ccggcaggcc gaacaacctg gccggcctgg
     3181 tcgaaagcag gtcgaccagc gtcaggaggg agagatcgcc ggcgcgtacc agccgcagca
     3241 gcagcggcag cgccagctcg aagccaatca tgcccgaggc cgcgcggcgc aggtcgcccg
     3301 gcttgtcctc cggcgcgtgc ggcgcgtggt cggtcgccag gatgtcgaag gtgccctcgc
     3361 gcagcccggc cagcagcgcg cgagcatcgg gctccgtgcg cagcggcgga ttgaccttgg
     3421 cgttggggtc ggggcggacg gtggcataga cgcattcgtc gcccacgaag cggcgccggc
     3481 cggcgaccca ttcatcggtc aagaggagat gatgcggcat cacctcggcg gtgacgtgca
     3541 ccccctgctc cttcgcgcgc cgcaccagat cgcggcccag ctccgtgctg acgtgcagca
     3601 cgtgcagcca gccgccggtc aggcgcgcca gctcgatgtc gcggctggtg atgatctcct
     3661 cggcggcggc cgggatgccg ggcaggccca gctcgcgcga gaccgcgccc tcgtgcatca
     3721 ccccgccgtt gatcagcgtc cagtcctcgc agtggaccat gatcggcttg cccgatgcca
     3781 ccgaccaggc cagcgcctcc cgcatcacct gcgagctgcg cgtgctgtcg ccgtcgtccg
     3841 agaagccgac cgcgccggcc tcggtcatgc cggccagatc ggccaactcc tcgccgcgcc
     3901 gccccagcga gatcgcgccg agcgggaaga gccgcagcgg gagctcgcga gcgcgcgcca
     3961 cgatgtcggc cacgcgctcg ggcgtgtcga gcgccggatt ggtgttgggc atgcagcaga
     4021 ctgtggtgaa gccgccctgg agggcggatt gcgcgccggt ctccagcgtc tccttggcgg
     4081 tgaagccggg gtcgcgcagg tgggcgtgga catcgaccag gccgggcatg acgagcaggt
     4141 tggcggcgtc gaactcctca gccccctcag gctgggtcag accccggccg atcgcggcaa
     4201 tgcgcccatc ctgaacgagc agatcggcgc gctcgtccag cccctgctcg ggatcgataa
     4261 tccgaccccc gcgaaacatc accgccccca tagccccctc accgctccgt cccggccgca
     4321 agccgcgcat aaaaaaaggc ccctgggctc gaaaggagtc caaaggccag tcgccacgtc
     4381 gcaggttgcg cgtacgctat ccaggtgtcg gtctcgctca gcaaatgtcc gtccctgggt
     4441 ccgtagacga ccgacttcgg tgcatccgag cgattatacc aggagcgacc gccagtctca
     4501 agggtggagc ggaccaagtg tacgttcaat cccaggcgat tcccctcggc tgcgagggcc
     4561 gcccagcggc gccgcctcag ccgcttcgcg cttggcgctc cgtgctggtt gatctcgaca
     4621 agccgccgcc acgatggcgt gcgactcgct gcgccacgta ccagacggcg gcggccagca
     4681 ccaacccggc cacgacatcg ctcaggtagt gctcgccgag atagaccagg gcgaacccca
     4741 tcgccagtat gtagacgacc gccaggatcg cgccgagcgg cgccctccag cgcgctagca
     4801 cgaaagcgac gacgcagatc ccggccatgt gcagggacgg catcgctgcc acgtcgtttt
     4861 ggccggcaag ctgatcgccg atgccgaagc tgccgggaat gatgtggttg atcgcggtcg
     4921 ggaccacgcg catgaacggc ggcaggtagc cgcgctcaga tgccagccag ggaggggccg
     4981 tcgggaccaa gaaaatgggg atgagcccga catagagcgt gccgaagaag gccaggacat
     5041 aacggacggt ccggacgtcc cggcgcagcc agaagtagag cgcgacgaga tggggcacga
     5101 agaagtaggt cacgtagacc gccaccagca gggaatccaa ccaattgata tggccggcct
     5161 gacgcagtgt cgcttgaagc cagacggtcg gatcgtgccc ggcaaagagg gcgtgatcga
     5221 cctgaattgg gtagcggaag cgcacgggta taccggtctg gttggcgaac gcgcgcagcc
     5281 gcacgaagat cacgtaggcg gccaggtaga ggatcggtat ccatacgtag cgcgacctga
     5341 gccgcgcgcg agccctgtgg gtcgaccggc aatggttgtc ttccctatcg ccgggtcggc
     5401 tgccggtcat gcgttcgccc taccgtgcct cgcgggatta cccctgggca aaccgtgttc
     5461 cttcaacatc ggtgccgctg ggcgggccgg ttgcactgtg gaacccgtgc gctcacggaa
     5521 tcagcggcag cgtgcgacgc agcagcgccc aggcacggcg gccgatctca tccaggtgcg
     5581 gctccagcgt gggcaagtca tcgcccagct cggtgacggc gagcagctcg ttgttggaga
     5641 tgtccttgac cgtcagaaac ggcactgacc agagatggca gacctgggcc agcgcggcgg
     5701 cctccatgtc ctcgcagagc gagccgtggc ggccgtgcag cgcctcgatg cgcggcgcgt
     5761 gctgggtcca gcagtcggcc gaggcgacga caccgcgctc gacaacgggc ggtctgctgc
     5821 cgcgtggcga acccggccac cgctccggct gccagccctc cgcggcgtgc cgtgccccgg
     5881 cgagcaagcc cgggtcagcg tcgatctcct cgaccggctt gtcctctgcc cccagggcac
     5941 agaagaggcc cgtgtagcgc tcctcgccgc ccggcagaat cgtcagcgac tgcggcgcga
     6001 ccacgcgctc gccaatgacc acgtcgccgg gcaggaggtc ggcccggtgc gccccggcac
     6061 agccgtagtt cagcacggcc gccggtcgct cgaccgccag cagcgccgag agcgccgcgc
     6121 cggcgttgac catgccgatg ccgctacgca ccagtcgcac ggctcgacgc tcgatagaac
     6181 cctcggtgat ggtccagggg ccaatgcgcc gctcgccggt gatctcggtg gcctccatcg
     6241 cgtggcgcaa ctccgtcttc atggcgcaga cgatcgccag c
//
LOCUS       DASUBI010000372         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1069625, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000372 DASUBI010000000
VERSION     DASUBI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069625"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..483)
                     /locus_tag="VFU72_07205"
     CDS             complement(64..483)
                     /locus_tag="VFU72_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5423308.1"
                     /translation="MPTLPYLEEEDAGDEVRRLYQEIKAGFGAGQVPNFYKVLGNNAD
                     MLAAALDNRRRIMEQGQLDPTLKEFLAWASVTLANNEFGIKVHTARLKKLGYGNAQIL
                     EALAVLQYFTGVSAVINGLAMGDDVNSSVLKYLESAQ"
     gene            complement(495..971)
                     /locus_tag="VFU72_07210"
     CDS             complement(495..971)
                     /locus_tag="VFU72_07210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423309.1"
                     /translation="MSTSQPAGSEPGLIRTWVARSLDEMPDIYSVRHEVFVVEQGLTH
                     TVVDEPDDRYSVHVLAAIGAQIVGVGRVTFIGDEAQIAWVAVRRAYRRHGVGRAIMDL
                     LLEVSREQTSLVSLNAQTHALHFYESLGFRPVGRRFHMAQIEHQHMVMELPPAGQQ"
     gene            complement(943..>2382)
                     /gene="argH"
                     /locus_tag="VFU72_07215"
     CDS             complement(943..>2382)
                     /gene="argH"
                     /locus_tag="VFU72_07215"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5423310.1"
                     /translation="TDRLVDRLNASLPYDRRFYREDIGGSIAHARMLARQGIITTADA
                     EVIEAGLRAVLAEIERGDYEFRLEDEDIHLSVERRLRELIGSPAGRLHTARSRNDQAA
                     LDFRLWIRAALLRLAAGVAESIAALLELAARYPEAIMPGYTHLQRAQPVLLAHHLQAY
                     AAMLFRDLDRIRDAERRINQSPLGAGALAGVTYPIDRAFVAELLGFDGVAVNSMDAVA
                     DRDYVVDTLSACSQIMMHLSRLAEDLILWSTTEFGFVELDDAFATGSSIMPQKKNPDV
                     AELIRGKAGRVYGHLMAMLTAAKGLPLTYNKDLQEDKEALFDAVDTVETILDVLPPML
                     RGATFRVERMAEAAGGGFALATDIADHLARQGVPFREAHEIVGRLVAYCVANGKDFPD
                     LAVSEWADFSPLLAESPPPVTVRDAIAAREMLGGTGFRAVAAERDRARAELASWQRWI
                     AGRLDREAAVAARLGFENTSDEHVAAGGQ"
BASE COUNT          373 a          842 c          790 g          377 t
ORIGIN      
        1 cccgagcgca gtcgagagat ccgtgcccca ggcgccaccc aatctacgcg accgtcccga
       61 tctctactgc gcgctctcca aatacttcag taccgacgaa ttcacgtcgt cgcccatggc
      121 caaaccattg atcacggccg agacgccggt gaagtactgc aagaccgcca gcgcctccag
      181 gatctgggcg ttgccgtagc ccagcttctt gaggcgcgcg gtgtgaacct tgataccaaa
      241 ctcgttattc gccagcgtca ccgaggccca ggccaggaac tctttcagag tggggtcgag
      301 ctgcccctgc tccatgatgc ggcgccggtt gtcgagcgcg gccgccagca tgtccgcgtt
      361 gttgccgagc accttgtaga agttcggcac ttggccggcg ccgaagccgg ccttgatctc
      421 ctggtacagc cgccggacct cgtcgccggc gtcttcttcc tccagatagg gcagcgtcgg
      481 catcggcttc tccttcactg ttggccagcc ggtggcagct ccatcaccat gtgctgatgc
      541 tcgatctggg ccatgtggaa gcggcgcccg accgggcgga agccgagcga ttcgtagaag
      601 tgcagggcgt gcgtctgggc gttcaggctg acgagactgg tctgctcgcg gctgacctcc
      661 agaagcagat ccatgatggc gcggcccacc ccatgccggc ggtaggcccg ccgcaccgcc
      721 acccaggcaa tctgcgcctc gtcgccgatg aaggtcaccc gcccgacgcc gacgatctgc
      781 gcgccgatgg ccgccagcac gtgcacgctg tagcggtcgt ccggctcgtc gacgaccgta
      841 tgggtcaggc cctgctcgac cacgaagacc tcgtgccgca ccgagtagat gtcaggcatc
      901 tcgtcgagcg agcgtgccac ccaggtacgg atcagcccgg gttcactgcc cgccggctgc
      961 gacgtgctca tcgctcgtat tctcgaagcc gaggcgtgcc gcgaccgcgg cttcgcggtc
     1021 cagccgcccg gcgatccagc gctgccagga cgcgagttcg gctcgcgccc ggtcccgctc
     1081 cgctgccacg gcgcggaatc cggttcctcc cagcatctcc cgcgccgcga tcgcgtcccg
     1141 cacggtgacc ggcggcggac tctcggccag cagcggcgag aagtcagccc actccgacac
     1201 cgccaggtcg ggaaagtcct tgccattggc gacgcagtag gcgaccaggc gcccgacgat
     1261 ttcgtgcgcc tcgcggaagg gcaccccctg gcgcgccagg tggtcggcga tgtcggtggc
     1321 cagcgcgaag ccgccgccgg ccgcctcggc catgcgctcg acgcggaagg tggcgccgcg
     1381 cagcatcggc ggcagcacgt cgaggatcgt ctcgacggtg tcgaccgcgt cgaacagcgc
     1441 ctctttgtcc tcctgcagat ccttgttgta ggtcagcggc agccccttgg cggcggtcag
     1501 catcgccatc aggtgcccgt agacccgccc cgccttgccg cggatcagct cggcgacgtc
     1561 gggattcttc ttctgcggca tgatgctgct gccggtggcg aaggcgtcgt ccaactccac
     1621 aaacccgaac tcggtggtgg accagaggat caggtcttcc gccaggcgcg agaggtgcat
     1681 catgatctgg ctgcaggccg agagcgtgtc gaccacgtaa tcgcggtcgg ccaccgcgtc
     1741 catgctgttg accgccaccc cgtcgaagcc cagcagctcg gccacgaacg cgcgatcgat
     1801 cgggtaggtc acgcccgcca gcgcgccggc gcccagcggc gactggttga tccggcgctc
     1861 ggcatcgcgg atacggtcga ggtcgcggaa aagcatggcg gcgtaggcct gcaggtggtg
     1921 cgccagcagc accggctggg cgcgctgcag gtgggtgtaa ccgggcatga tcgcctcggg
     1981 gtagcgcgcg gccaactcca gcagcgcggc gatcgactcg gccacgcccg cggccagccg
     2041 cagcagggcg gcgcggatcc agagacggaa atcgagcgcg gcctggtcgt tgcggctacg
     2101 ggcggtgtgc aggcgcccgg ccggagaacc gatcagctca cgcagacgcc gctcgacgct
     2161 caggtggatg tcctcgtctt ccaagcggaa ctcgtagtca ccccgctcga tctcggccag
     2221 caccgcgcgc agccccgcct caatcacctc ggcgtcagcg gtggtgatga tcccctgccg
     2281 cgccagcatg cgcgcatggg cgatcgagcc cccgatgtcc tcgcggtaga agcgccggtc
     2341 gtaggggagc gaggcgttca ggcgatcgac caggcgatcg gt
//
LOCUS       DASUBI010000373         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_552795, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000373 DASUBI010000000
VERSION     DASUBI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552795"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..931)
                     /gene="ubiE"
                     /locus_tag="VFU72_07220"
     CDS             complement(188..931)
                     /gene="ubiE"
                     /locus_tag="VFU72_07220"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEU5423311.1"
                     /translation="MTTAETETETSEAASGALKRPPEVRSMFDRISWRYDLMNTLMTA
                     GRHHDWRRRAARAAIGAGAARVLDVATGTGDLALELAGQGVALVVGADFSGGMLASAA
                     AKRAESGTQAVRLVQADAMRLPFASGSFDAVTIGFGLRNLPDYGGAVAELARVLRPGG
                     RLVILEMSPLRQPLLRRGFDLYFAHLVPLVGGLISGDRDAYGYLPRSVGAFPPPEQLM
                     DLMRQAGLRSVRYESLALGTVALHIGVHR"
     gene            1157..2110
                     /locus_tag="VFU72_07225"
     CDS             1157..2110
                     /locus_tag="VFU72_07225"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5423312.1"
                     /translation="MTTTSGSGAGPLAAELNNQGQFVRQEYSIRDRISADGSSGFPAE
                     PGRYHLYVSLACPWAHRSIIARRLLGLEDTVSLSVVDPVRDERGWAFSEGFADPVNGF
                     KFLAEAYHATDPDYVGRYTVPCLWDRASGRLVSNNFPDLTLDFETQFTDYHKPGAPDL
                     YPEALRPQIDAMNEVIYQDVNNGVYRAGFATSQAAYDEAVTALFARLDWLEQRLAGQR
                     YLVGDQLTEADIRLFPTLVRFDAVYYLHFKCNLRRLVDYPNLWAYARDLYQRPGFGDT
                     VDFDHIKRHYYTTHPQINPTRIVPKGPIVDWDAPHERARLG"
     gene            complement(2130..2804)
                     /locus_tag="VFU72_07230"
     CDS             complement(2130..2804)
                     /locus_tag="VFU72_07230"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5423313.1"
                     /translation="MDELDITESSAEARDTGRIEAFSDGVFAIAATLLVLQVAVPESS
                     TNLLRTVLHQWPAFLAYAISFVTILIMWINHNMIMRQIGHADHGFKVLNGALLMAITF
                     VNYPTALLARYLTTPQERDAALIFNGTYVVVALCFLALWRYSAHRGRLLSRQADPRLV
                     AAITRSYRFGPLIYLVATIAAFASVPVSLAINGGLALYWAIFGARAVGEFGAGATARR
                     PRWRTR"
     gene            complement(2968..3915)
                     /locus_tag="VFU72_07235"
     CDS             complement(2968..3915)
                     /locus_tag="VFU72_07235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5423314.1"
                     /translation="MKTIGFVGVGSIGLPMARRLLAAGYPLVFASSREPACQELEALG
                     GRAVPTPPVVAEECEVFMTALPSDRALSEVYLGPGSVLEHLRPGSIVVDFSTASPMIL
                     QRVDTEARGRNIRVIDAPVSGGISGAEQGTLTIMAGGEPDVVEEILPIFETLGERVFI
                     VGDVGMGKVFKIINNLLTGATYVLVGEALALAACAGADLDLLHEVIAVSSGASRAWGD
                     AVPKLVHRADGEQASFRLELMRKDLRLAAGLGEDLATPTPLTSLALQFYTAAVSRGLG
                     RQDAYTVARLVADLARANLTAADEPGAATRDGDLAAGDE"
     gene            complement(4008..>4517)
                     /locus_tag="VFU72_07240"
     CDS             complement(4008..>4517)
                     /locus_tag="VFU72_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEU5423315.1"
                     /translation="LMRRGLLVGTILRSALRQGEVIYEMDDRELRAVTAQWLQYARED
                     LAVAESMSRGTVATPRHICFQAQQAAEMALKAALVYLQIDFPRTHELDVLRGLIPAGW
                     AAREALPELTRLSSWSVRERSPGSAPAVPQEIPICLDLARDVLDVVEQDLRRQGFTAP
                     DDAGHASEQ"
BASE COUNT          701 a         1573 c         1604 g          639 t
ORIGIN      
        1 gaggagatcc tggcgcgggc gctgggcgaa ccagagggcg acgcggattt cctgtagggg
       61 caggcccctg tgcctgccct gctgccccta cccggaagca acggggcaga ggtgcgcgac
      121 ggtcggcgct gatgcgggcc ggtgccaggg cgacgagggc agacacggct ccgttcgagg
      181 tctgccccta ccgatgtacc ccgatgtgga gggcgacggt gccgagggcc agcgactcgt
      241 agcgcacgct gcgcagcccg gcctggcgca tgaggtccat gagctgctcg gggggtggga
      301 aggcgccgac cgagcgcggc aggtagccgt aggcgtcgcg gtcgccgctg atcagcccgc
      361 cgaccagcgg caccaggtgc gcgaaataga ggtcgaagcc gcggcgcagg agcggctgcc
      421 gcagcggcga catctccagg atgaccagcc gcccgccggg ccgcagcacg cgcgccagct
      481 cggccaccgc gccgccgtag tcgggcaggt tgcggagtcc gaagccgatc gtgaccgcgt
      541 cgaagctgcc gctggcgaag gggagccgca tggcgtcggc ctgtaccaac cgcacggcct
      601 gcgtaccgga ctcggcgcgc ttggcggcgg cgctggccag catgccgccg gagaagtccg
      661 cgccgaccac cagcgccacc ccctgccccg ccagctccag cgccagatcg ccggtgccgg
      721 tcgccacgtc gagcacgcgt gcggcgccgg ccccaatggc ggcacgggcc gcccgccggc
      781 gccagtcgtg gtgccggccg gcggtcatca gggtgttcat caggtcgtag cgccaggaga
      841 tgcggtcgaa catcgagcgg acttcggggg gccgcttgag ggcgccgctc gccgcctcgc
      901 tggtttcggt ctcggtctcg gccgttgtca cgggatgctc cttcatgcgg caatcatacg
      961 gcgcgggcga gtcctcaccc ccacgcaccg gccacggggg catggagaac gtgggaggcg
     1021 gacgtgaggg ccggccgtgg gggcggtagg gcaggcacgg ggccggcccc taccggtggg
     1081 gtggccgggg aatgcgcgcc atctggcacc ggttgccaga tagcggtacg ctgagaaccg
     1141 aaaggagctg aagattatga caactacgag cggcagcggt gcgggaccgc tggcggcgga
     1201 gctgaataat caggggcagt tcgtgcgcca ggagtacagc atccgcgacc gcatcagcgc
     1261 cgatggctcg tcgggtttcc cggccgagcc ggggcgttac cacctctacg tctcactcgc
     1321 ctgcccctgg gcgcaccgct cgatcatcgc gcggcggctg ctcgggctgg aggataccgt
     1381 ctcgctctcg gtggtcgacc cggtgcgtga cgagcgcggc tgggccttca gcgagggctt
     1441 cgcggacccg gtcaacggct tcaagttcct ggccgaggcc taccacgcca ccgatcccga
     1501 ctacgtcggc cgctacaccg tcccctgcct ctgggaccgt gcatccggcc ggctggtcag
     1561 caacaatttc cccgacctga cgctcgactt cgagacgcag ttcaccgact accacaagcc
     1621 gggcgcgccc gatctctatc ccgaggcgct gcggccgcag atcgacgcga tgaacgaggt
     1681 gatctaccag gacgtcaaca atggcgtcta ccgcgccggc ttcgccacct cgcaggcggc
     1741 ctacgacgag gcggtcacgg cgctctttgc ccggctcgac tggctggagc agcggttggc
     1801 ggggcagcgc tacctggtcg gcgatcagct gaccgaggcc gacatccgcc tcttcccgac
     1861 gctggtgcgc ttcgacgcgg tctactacct gcacttcaag tgcaacctgc gccgcctggt
     1921 cgactacccc aacctgtggg cctacgcccg cgacctctac cagcggcccg gcttcggcga
     1981 cacggtcgat ttcgaccaca tcaagcggca ttactacacc acgcacccgc agatcaaccc
     2041 gacgcgcatc gtgcctaagg ggccgatcgt cgattgggac gcgccgcacg agcgcgcgcg
     2101 gctggggtag cccgtcggcc gggcggcaat cagcgcgtgc gccaacgcgg gcggcgggcc
     2161 gtcgccccgg cgccgaactc gcccacggca cgcgcgccga agatggccca gtagagcgcc
     2221 agcccgccgt tgatcgccag cgagacgggc acgctggcga aggcggcgat cgtggcgacg
     2281 agatagatca gggggccgaa gcggtaggag cgggtgatcg ccgcgaccag gcgcgggtcg
     2341 gcctgccgcg agagtagccg gccgcggtgc gcggaatagc gccacagcgc gaggaagcag
     2401 agcgcgacca ccacgtaggt gccgttgaag atcagggcgg cgtcgcgctc ctggggcgtg
     2461 gtgaggtagc gcgccagcag cgcggtcggg tagttcacga aggtgatcgc catcagcagc
     2521 gcgccgttga gcaccttgaa gccgtggtcg gcgtgcccga tctggcgcat gatcatgttg
     2581 tggttgatcc acatgatcaa gatggtcacg aagctgatgg cgtaggccag gaaggcgggc
     2641 cactggtgca ggaccgtgcg caacaggttg gtcgaactct ccgggaccgc cacctggagg
     2701 accagcaggg tcgccgcgat ggcgaagacg ccgtcgctga aggcctcgat gcggccggtg
     2761 tcgcgggctt cggcgcttga ctcggtgatg tccagttcgt ccacacgcgt ctccgtcggg
     2821 ccctcacccc ctgcccgcac ggagattctc ccaatgctgg tcgaggggag acgagggtgg
     2881 gcgggtttga aacccgcccc tacaatccgt cgtgctcccc cgtctcccag tattgggaga
     2941 agggggcggg gggatgaggg ccgtctccta ctcgtcgccc gcggcgaggt cgccatcacg
     3001 cgtggcggcg cccggctcgt ccgcagcggt tagattggcg cgggccaggt cggccaccag
     3061 gcgcgccacc gtataggcgt cctggcggcc cagcccgcgc gagaccgccg cggtgtagaa
     3121 ctgcagagcc agcgaggtca gcggcgtcgg cgtagccagg tcctcgccca gcccggcggc
     3181 caggcgcagg tccttgcgca tcagctccag ccggaaactg gcctgctcgc cgtccgcgcg
     3241 gtggaccagc ttgggcacgg cgtcgcccca ggcgcgcgag gcgccggagc tgaccgcgat
     3301 cacctcgtgc agcaggtcga ggtcggcacc ggcgcaggcc gccagcgcga gcgcctcgcc
     3361 caccagcacg taggtggcgc cggtcagcag gttgttgatg atcttgaaga ccttgcccat
     3421 gccgacgtcg ccgacgatga agacgcgctc gcccagcgtc tcgaagatcg gcaggatctc
     3481 ctcgaccacg tccggctcgc cgccggccat gatcgtcagc gtgccctgct cggcgccgga
     3541 gatgccgccg ctgaccgggg cgtcgatcac ccggatgttg cgcccgcgcg cctcggtgtc
     3601 gacgcgctgc aggatcatcg gcgaggcggt gctgaagtcg accacgatgc tgccggggcg
     3661 caggtgctcc agcacgcttc cggggcccag gtagacctcg ctcagcgcgc ggtcggaggg
     3721 gagggcggtc atgaagacct cgcactcctc ggccaccacc ggcggcgtcg gcaccgcgcg
     3781 cccgccgagc gcctccagct cctggcaggc cggctcgcgg ctgctggcga agacgagcgg
     3841 gtagccggcc gcgaggagcc gccgggccat cggcagcccg atcgaaccga ccccgacgaa
     3901 cccgatggtc ttcatgccgc gctcctcacc aatctgccgc cttgggtcaa tggccgcacc
     3961 cgcccctcat ccgctgcaat cctaccgagt gcggcaagat cgggagtcta ctgctcgctg
     4021 gcatgccccg catcatccgg cgcggtgaag ccctggcgcc gcagatcctg ctcgacgacg
     4081 tcgaggacgt cgcgcgccaa gtccaagcag atggggattt cctgcggcac ggccggtgcc
     4141 gagccggggg agcgctcacg gaccgaccag gacgacaatc gcgtcaactc cggcagcgct
     4201 tcccgggcgg cccagccggc cgggatcaag ccgcgcagca catcgagctc atgcgtgcgc
     4261 ggaaagtcga tctgcaggta aacgagcgcg gctttgagcg ccatctctgc cgcttgctgg
     4321 gcctgaaaac agatgtggcg cggcgtagcg accgtgcccc tgctcattga ttccgccacc
     4381 gccaggtctt cgcgcgcgta ctgcagccat tgggcggtca ccgctcgtag ctcccggtca
     4441 tccatctcgt agatcacctc cccttggcgc agagctgagc gcagaatcgt gccgaccagg
     4501 agtcctcgcc gcatgag
//
LOCUS       DASUBI010000374         8569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_310732, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000374 DASUBI010000000
VERSION     DASUBI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310732"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFU72_07245"
     CDS             complement(<1..290)
                     /locus_tag="VFU72_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423316.1"
                     /translation="MIDRVRTLRDRFRPPAPATAGRRLSRRQREERQRRLVIYGIIAS
                     GILVVALLAGGAAYQYLYLPNQTLAAVNGTKISRSAYWKYRKYELINQINQYS"
     gene            complement(393..593)
                     /gene="rpsR"
                     /locus_tag="VFU72_07250"
                     /pseudo
     CDS             complement(393..593)
                     /gene="rpsR"
                     /locus_tag="VFU72_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(764..1183)
                     /gene="ssb"
                     /locus_tag="VFU72_07255"
     CDS             complement(764..1183)
                     /gene="ssb"
                     /locus_tag="VFU72_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5423317.1"
                     /translation="MSRGLNKIMLIGHVGREPEMRYTPSGKPVTEFSLAVNRRRRGSE
                     GEATDETDWFRIIAWDRLAEIVDQYVTKGRQVYVEGRLQIRRYTDREGQERTAVEVVA
                     NDLQMLGPRMESTGGGGSREPGGEPEFEKDEFDDVPF"
     gene            complement(1221..1673)
                     /gene="rpsF"
                     /locus_tag="VFU72_07260"
     CDS             complement(1221..1673)
                     /gene="rpsF"
                     /locus_tag="VFU72_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5423318.1"
                     /translation="MVLVMPELADEALTAEVEQISGFLNGNGAEITLLKRDTPWGRRR
                     LAYPIERFRDATYVLYRFNAPPGAVRAIERELQLDERVIRYLFVRQESAQEEAEAEAE
                     EAPQTTEGEATPAAVQAQVEPAPAEAEPDGETEPAAEPPEEPEPAESV"
     gene            2085..2465
                     /locus_tag="VFU72_07265"
     CDS             2085..2465
                     /locus_tag="VFU72_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423319.1"
                     /translation="MADDDPAIRALLTELLTDEGYDVLEAVDGRAAVEIALALQPQVI
                     LMDLRMPHIDGIDATRTLKADPRADGIRIIAMSAGYNLRSVADQLPADSLIAKPFDLD
                     ALLADVAVQLHHAAAATQSLTEHA"
     gene            2534..3121
                     /locus_tag="VFU72_07270"
     CDS             2534..3121
                     /locus_tag="VFU72_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5423320.1"
                     /translation="MDDERRQEIANWARTIQRRRDVAFLDTETTGLRHDDEIIDIALV
                     DASGRTLLNTLVRPSRPIPAEVIAIHGISDAHVAGSPRWPEVYPELCRLLQEFHCLVI
                     YNAPFDLRLLDQTCRRFQLSPLSASIEAHCAMRQYARYAGLGKRSRLQDALTAVGARA
                     NLAHRALADANACRTLVGAMARDGACRTIPGVGRR"
     gene            complement(3231..4073)
                     /locus_tag="VFU72_07275"
     CDS             complement(3231..4073)
                     /locus_tag="VFU72_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423321.1"
                     /translation="MDGCLRGCLVVIAVLVLLPFLPIILLGFVVFVVGRFLLGFWQSD
                     RPLRTKRAVTGLVFYPAGAYFLYRDTRYPPKLKHAFLAAAIVATVVAVNASALLLPLA
                     VALGAVFLALYMAGTQAGAPEAATVKITPARTVGERRLEVPSDDMRRLLEIEEARGED
                     ERRLLLAREFNRLATEACGPAPLDRAFWPDRQRLGVLRVEIGLLRASALETPAALRAP
                     QDEVVDYADLRVAIDSLRGYVGMMLALDGQEHPDPEYLRRLARERGRIQMVQDELLSR
                     LQKS"
     gene            complement(4164..4874)
                     /locus_tag="VFU72_07280"
     CDS             complement(4164..4874)
                     /locus_tag="VFU72_07280"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEU5423322.1"
                     /translation="MTFTLVARCPRTGMLGIGTATRAYAAGVRVPFVRANLGAVAIMA
                     IADERLGPTALRFLELGYKAPGVIEELVRADPFPEYRQLAVVDADGFAAARTGAKNRA
                     WAGHRLGDGYVVLGNAIAGEAVVDAMEAAFRAEPGEDLEERLLRSVEAGRDAGGQPGG
                     QRSAALLVFDRKTFSRVDLRVDLHPEPVGELRRIFDQYRPAIPYYEERQVDPRVMPLD
                     DWLVAHGAEPAPAVPFGG"
     gene            5017..5538
                     /locus_tag="VFU72_07285"
     CDS             5017..5538
                     /locus_tag="VFU72_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423323.1"
                     /translation="MSITPTPPRGRDRDRYLGWVPVGLVGGELGGLGLALTSMIWTWF
                     NGLGFWYPLSLFGSLLLGESAVSHGAAASADLLGVAVLVVLSALFGLVFTYGVAHTGL
                     AQGAIIALGVGWGLLLWAIGVAFIDAQFIPAGLSDAPTSMMVVSFIVWGFLLGLVTAM
                     SLARSPHLIRQRP"
     gene            complement(5543..6409)
                     /locus_tag="VFU72_07290"
     CDS             complement(5543..6409)
                     /locus_tag="VFU72_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5423324.1"
                     /translation="MAQNETGTRGPRDNDTGYAGRRIQPEQNWSYTPPGSQVDLNEPI
                     WHAENYNRGAFAGWPNHNWDNERPTSGLWQVRGPYIGKGPRTERQDVFIQEQISENLA
                     EHPQLDASEITVFVEHGEVTLEGSVDSRWAKRQAEQAADSVPGVKDVHNRLAIEPVAE
                     EGWRRGVAPQPLGTISGAIEPGMRQPMLTSAGTNAGAPGTGLGLRGGPAAASGRLRTG
                     MYVMGADGHQIGTIKAVRGDDFLVDRNLQRDVYVPFRAVQAIQGDRITLSVSGPGVDA
                     RNWPRPSLFGGA"
     gene            complement(6483..7514)
                     /locus_tag="VFU72_07295"
     CDS             complement(6483..7514)
                     /locus_tag="VFU72_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5423325.1"
                     /translation="MRIGMVAPLVEPVPPPLYGGTERVVSVLTEELVRRGHDVTLFAT
                     GDSETNAHLIACAPRGLRLDDQVHDYNAFTIIELGEVYQRAQEFDVIHNHTDYFGFPF
                     ARLTETPTVTTTHGRLDLPEVQRVYQLFPEQPLVSISDAQRAPLRGQNWQATVYNGIE
                     LDNYTFRSEQGDYLVFLGRISPEKRPDRAIEIARDTGMPLIIAAKVDPVDQAYYEQAI
                     RPLIESSRLIEYIGEVDEREKDKLLGEAYAYLFPIDWPEPFGLTMVESMATGTPVIAS
                     AVGSVPEVVVDGVTGFVRQSLHDMVDAVERVGQLDRAACRAHVEACFSAEAMARGYEQ
                     VYQRLAAHA"
     gene            7693..8310
                     /locus_tag="VFU72_07300"
     CDS             7693..8310
                     /locus_tag="VFU72_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423326.1"
                     /translation="MAHTRVGIWKLTSGSDRDELAQVFREQIVPEFQQQPGFISYLAV
                     GSGRSQAAGVQTWETPEQAVQAGARTGDRVRQQIGGQATLESWFHGPVAIDIARQPLD
                     KARYARIGIWDIAPSANRQELTEKAREALIPMLQQASGLVSYRAIEGSEGRLVVVHCW
                     ESEEASERGMQSTGQWLQETLANQLALVARYDGPVLLAASAKQGG"
BASE COUNT         1374 a         3000 c         2804 g         1391 t
ORIGIN      
        1 gaatattgat tgatttggtt gatcagctcg tatttgcggt acttccagta ggccgagcgg
       61 ctgatcttgg tgccgttgac cgcggccagc gtctggttgg gcaagtagag gtattgatag
      121 gcggcgcccc cggcaaggag cgccacgacc aggatgcccg acgcgatgat gccgtagatg
      181 acgaggcgac gttgacgctc ctcgcgctgc cggcgcgaga gccgccggcc ggccgtcgca
      241 ggcgccggcg gacggaagcg gtcgcgcaga gtgcgcacac gatcgatcat gctactgctt
      301 tcgtctcaag ccgtatcaaa ggcatgttga gcagagcaaa ccgcgcccag cctcggccgg
      361 tccagccgtc cgaagccagg acacacggtt gactattggg tgaacggaag cagcccaacg
      421 tggcgcgcgc gcttgatcgc gaccgccagc gagcgctggt gcttggcaca ggtgccggtc
      481 tgccgtcgcg gctcgatctt gccacggtcg gagacgtagc ggcgcaggcg cccgaagtcc
      541 ttgtagtcca cctggccgat gtgatccatg cagaaatggc acaccttgcg ccgcgggtag
      601 tatcggccac caccgccacc aaagcgccgt cgcggccgct caccgcccgc gcgctcgcca
      661 cgctccggcc gctcggagcg atccgagcgc tccgggcgcg ccgtcatctc acgtcgttcc
      721 tctgacacgt attcctcctc gtatcgctcg acgcgcgtcc tacctagaac ggcacgtcgt
      781 cgaactcgtc cttttcaaac tcgggttcgc cgcccggctc ccggctaccg ccgccgccgg
      841 tcgactccat gcgcgggcca agcatctgca aatcgttggc aacgacctca accgcggtgc
      901 gctcctgccc ctcgcggtcg gtgtagcgcc ggatctgcaa acggccttcc acgtagacct
      961 gacggccctt ggtcacatac tgatcgacga tctcggccag ccggtcccag gcgataatcc
     1021 ggaaccagtc ggtctcgtcc gtcgcctcac cctccgagcc gcgccgcctc cggttcaccg
     1081 cgagggagaa ctcagtgacc ggcttgccgc tcggggtata gcgcatctcg ggctctctcc
     1141 cgacatggcc gatcagcatg atcttgttca agccccgaga catcgcgcta ctcctctcgc
     1201 ttggcgatcc accgcattcg ctagaccgat tcggccggct cgggctcctc cggcggctcc
     1261 gcggcaggtt ctgtctcgcc gtcaggctct gcctcggcgg gcgccggctc tacctgcgcc
     1321 tggaccgccg cgggagtggc ctctccctca gtcgtctggg gagcttcttc cgcctcggcc
     1381 tcagcctcct cctgcgcgga ttcctgacgg acaaacaggt aacgaatgac gcgctcgtcg
     1441 agttgcagct cgcgctcgat cgcccgcacc gcgccagggg gcgcgttgaa gcggtagagc
     1501 acgtaggtgg catcgcggaa gcgctcgatc gggtacgcca gccggcgccg gccccagggg
     1561 gtatcacgct tgaggagagt gatctccgcg ccattgccat tgaggaagcc gctgatttgc
     1621 tcaacctccg cggtgagcgc ctcgtcggca agctcgggca tgaccaacac catcagttcg
     1681 tagggacgag gtatggagcg atactctcgc aacgatttcc ccttccatcg ggaatgcgcc
     1741 ggtttcaacg cggatcgttg cgcaccgcgt gagacaacag cgcagaaagg acaatcccgg
     1801 gtatacaccc gctggctccg gcccgtcgat tgccgggaac ccgcaaagta tatcactggc
     1861 gtgcatcctg acccaatcgt ggatttcgga gcgcctgtcc gggagctggg cggtggatga
     1921 ccggctggag cgcgccggcg gcgcacgaat aattcacttc gcgagatact gcgcgcgggg
     1981 atggccgaac gcctcgccgg gggtcgtttt ggcctggatt ttgcgattac atcacgcacg
     2041 ccttggctgg gatgagaagg gattgtcgag tgggacgaat cttggtggcc gacgatgatc
     2101 cggccatccg cgcgttgctc accgagctac tgaccgacga aggctatgac gtgctcgaag
     2161 cggtcgacgg gagagccgcc gttgagattg ccctcgcgct gcagccacag gtaatcttga
     2221 tggatctgcg catgccgcac atcgacggca tcgacgcgac gcgcacgctc aaggccgatc
     2281 cgcgtgccga cggcatccga atcatcgcca tgtcggctgg ctacaacctg cgcagcgtcg
     2341 ccgatcagct tccggccgac agtttgatcg ctaaaccgtt cgatctcgat gccttgctgg
     2401 ccgacgtcgc cgtgcagctc caccacgccg cggccgcgac ccagtcgctc accgagcacg
     2461 cctgactcgc ttcgcttgcc gccctgcccg gctcgcgcta tcgtcgactc atccgatgtg
     2521 aagggatggg acgatggacg acgagcggag acaggaaatc gcgaactggg cgcgcacgat
     2581 ccagaggcgg cgcgatgtcg cctttctcga caccgagacg accggcctac gccacgacga
     2641 tgagatcatc gacatcgcgc tggtcgatgc gtccgggcgc acgctgctca acacgctggt
     2701 gcgccccagc cgaccaatcc ccgccgaggt catcgccatc cacggcatct ccgacgcaca
     2761 cgtcgccggc agcccgcgct ggcccgaggt ctaccccgag ctctgtcgcc tgctgcagga
     2821 attccactgc ctggtaatct acaatgcgcc gttcgatctg cggctgctcg accagacctg
     2881 ccggcgcttc cagttgtcgc cgctctcggc gtcgatcgag gcgcactgcg cgatgcgcca
     2941 gtacgcgcgc tacgccggtc tgggcaagcg ctcgcgactc caggacgcgc tgaccgcggt
     3001 tggcgccaga gccaacctgg cccaccgcgc cctggccgat gccaacgcct gtcgcaccct
     3061 ggtcggtgcc atggcacgcg acggcgcctg ccgcacgata cccggcgtcg gcaggcgcta
     3121 gagcgccggc cacgaaacgc caagcaactg ggcaacacca aagagatcct tggctgcgca
     3181 cctcaagccg gaggcggcgc gccgtcctac acctgccccg gacctgcgcg ctaggacttc
     3241 tgcagccggg agagcagctc gtcctgcacc atttggatcc gcccgcgctc gcgtgccagc
     3301 cggcgcaggt actccgggtc ggggtgttcc tggccatcca atgccagcat catgcccacg
     3361 taaccgcgca ggctgtcgat cgccacgcgc aagtcggcgt agtcgaccac ctcgtcctgg
     3421 ggagcacgca gcgcggcggg agtctccaag gcgctggccc gtagcagtcc aatctccacg
     3481 cgcagcaccc cgagccgctg ccggtcgggc cagaaggcgc ggtccagcgg cgcgggcccg
     3541 caggcctcgg tcgccagccg gttgaactcc cgcgccagca gcagccggcg ctcgtcctcg
     3601 ccgcgtgcct cctcgatctc caacagtcgc cgcatgtcgt cggacggcac ctccagacgc
     3661 cgttctccga ccgtgcgcgc cggtgtgatc ttgaccgtgg ccgcttccgg agcgcccgcc
     3721 tgggtaccgg ccatatagag cgccaggaag acggcgccga gcgcgacggc caagggcaga
     3781 aggagcgcgc tggcgttgac cgcgacgacg gtcgcgacga tcgccgcggc caggaaggcg
     3841 tgcttgagct tgggtgggta gcgtgtgtcg cggtagagaa aatacgctcc ggccgggtag
     3901 aagaccagcc cggtgacggc gcgcttggtg cgcagcggcc ggtcggactg ccagaagcca
     3961 agcaggaagc ggcccaccac gaagaccacg aaacccagca ggatgatcgg caggaacggc
     4021 aagagcacga gcaccgcgat caccaccagg cagccacgga ggcatccatc catcgtcgaa
     4081 ccccctctcc gtgacggcag ccatacgctc tgagtatagc cgctcgcgcc gagtcgtcgc
     4141 cccgagccga aggggcgccg ccgctagccg ccgaatggga ccgcgggcgc cggctcggcc
     4201 ccgtgcgcga cgagccagtc gtcgagcggc atgacgcggg gatcaacctg gcgctcctcg
     4261 tagtagggga tcgccgggcg gtactgatca aagatacgcc gcagctcgcc aaccggttcc
     4321 gggtgcagat cgacccgcag atcgacgcgc gagaacgtct tgcgatcgaa gaccagcagc
     4381 gcggccgagc gttgcccgcc cggctgcccg ccggcgtccc gcccagcctc caccgagcgc
     4441 agcagccgct cttccagatc ctcgccgggt tcggccctga aggccgcctc catggcatcg
     4501 acgaccgctt ccccggcgat cgcgttcccg agcacgacgt aaccatcgcc gagccgatga
     4561 ccggcccagg cgcggttctt cgctcccgtg cgcgcggccg cgaagccgtc ggcgtccacc
     4621 accgcgagct ggcgatactc gggaaacgga tcggcgcgca ccagctcctc gatcacacct
     4681 ggggccttgt agcccagctc cagaaagcgc agcgccgtcg gacccaaccg ctcgtccgcg
     4741 atcgccatga tcgcgaccgc tcccagattg gctcgcacga acggcacgcg cacgccggcc
     4801 gcgtaggcgc gagtggccgt gccgatcccc agcatcccgg tgcgcgggca gcgcgccacc
     4861 agcgtgaacg tcatcgctcg actccctccc atgcctgccg catcacgaat gccatcatgg
     4921 tatccggttc cccacccccc cgcttcggca cgcggcgtgc atgctcgccc ccaagcacgg
     4981 ccgcgccgcg agcggccgcc ggagggagga gcacgcatgt cgatcacccc gacgccacct
     5041 cgagggcgcg accgcgaccg ctatctcggc tgggttccgg tcggactggt cggaggcgag
     5101 ctgggtggcc tggggctggc gctgaccagc atgatctgga cctggttcaa cggtctcgga
     5161 ttctggtatc cgctcagcct cttcggcagc ctcctgctcg gcgagagcgc cgtcagccac
     5221 ggcgccgcgg ccagtgccga tctgctcggt gtcgcggtcc tggttgtcct ctcggcgctc
     5281 ttcggcctcg tcttcacgta tggcgtggcc cacaccggcc tggcacaggg cgcgattatc
     5341 gcgctcgggg tcggctgggg cctgctcctc tgggccatcg gcgtggcctt catcgacgcc
     5401 cagttcatcc cggcgggcct gagcgacgcc ccgacctcga tgatggtcgt gtccttcatc
     5461 gtctggggct ttctcctcgg cctggtcacc gccatgtcgc tggcgcgctc gccgcacctg
     5521 attcgccagc gcccctgacg gcctacgcgc cgccgaagag cgacggccgg ggccagttcc
     5581 gggcatcgac acctggtccg ctgacgctga gcgtgatccg gtctccctgg atcgcctgga
     5641 ccgcgcggaa cggcacgtag acgtcgcgct gcaggttgcg gtcgaccaga aagtcgtcgc
     5701 cgcgcaccgc tttgatcgtg ccgatctgat gcccatcggc gcccatgacg tacatccccg
     5761 ttcgcagccg cccgcttgcc gccgccggac caccgcgcag acccaggccg gtccccggcg
     5821 cccccgcatt cgttccggcg ctggtaagca tcggctggcg catccccggc tcgatcgcgc
     5881 cgctgatggt gccaagcggc tgcggcgcga ccccccggcg ccagccttcc tcggctaccg
     5941 gctcgatcgc cagccggttg tggacgtcct tgactcccgg cacgctgtcg gcggcttgct
     6001 cggcctgccg cttggcccag cggctgtcga cgctcccctc cagcgtcacc tcaccatgct
     6061 cgacaaagac cgtgatctcg gaggcgtcga gctggggatg ctcggcgaga ttctccgaga
     6121 tctgctcctg gatgaagacg tcctggcgct cggtccgcgg acccttgccg atatatggcc
     6181 cgcgcacctg ccaaaggccg gaggtcgggc gctcgttgtc ccaattgtgg ttgggccagc
     6241 cggcgaaggc gccccggttg tagttctcgg cgtgccagat tggctcattc aaatcgacct
     6301 gtgagccggg gggcgtgtac gaccaattct gctcgggctg aatgcgccgc ccggcgtagc
     6361 cggtgtcgtt atctcgcggc cccctagtgc cagtctcgtt ctgcgccatc gctcgccctc
     6421 cgtcatcgac tgccggccgt tcaggtcagt tgtctcctga cgtgcaagcg ctatgccggg
     6481 gatcaggcat gggccgccaa ccgctggtag acctgctcgt agccgcgcgc catcgcctcg
     6541 gccgagaagc aggcctcgac gtgcgcgcgg caggcggcgc gatcgagctg acccacccgc
     6601 tcgaccgcat cgaccatgtc gtgcaacgac tgccgcacga agccggtgac accgtcgacc
     6661 accacctccg gtacagagcc gacagccgac gcgatgaccg gcgttccggt cgccatggac
     6721 tcgaccatgg tcaggccgaa cggctccggc cagtcgatcg ggaagagata ggcgtacgcc
     6781 tcgccgagca gtttatcctt ctcgcgctca tcgacctcac cgatgtattc gatcaaccgg
     6841 ctgctctcga tcagcgggcg aatcgcctgc tcgtagtagg cctggtcgac cgggtcgact
     6901 ttggccgcga tgatcagcgg catgccggtg tcccgggcga tctcgatcgc gcgatcgggg
     6961 cgcttctcgg gggatatccg gccgaggaag acgaggtagt cgccctgctc ggagcggaag
     7021 gtgtagttgt ccagctcgat gccgttgtag accgtggcct gccagttctg accgcggagc
     7081 ggcgcgcgct gcgcgtcgct gatcgacact agcggctgct cggggaagag ctgatacacc
     7141 cgctgtacct ccggcagatc gagccgcccg tgtgtcgtcg tcacggtcgg tgtctcggtc
     7201 agcctggcga agggaaagcc gaaatagtcg gtgtgattgt ggataacgtc gaactcttgc
     7261 gcgcgctggt agacctcgcc gagctcgatg atggtaaagg cattgtagtc gtgcacctga
     7321 tcgtccaggc gcagcccgcg cggggcgcag gcgatcaagt gggcgttcgt ctccgagtcg
     7381 ccggtggcga agagcgtcac gtcgtgaccg cgccggacca gctcctcggt caacacggag
     7441 acgacacgtt cggtgccgcc gtagagtggt ggcggcaccg gttcaactag cggcgcgacc
     7501 attccaattc gcatcgttct acctcccaag gccgggtctg tcgcccggca cccgggccga
     7561 tgcacgatcc atgccgatcg gcgacggggg tcggcacgtc gcctgcggag aacagtctcc
     7621 agctttggcg gcgaagcgag gcgggcgcgg cggcgcgcca tacggctgtg cgagcggcgg
     7681 aaaggaagcc cgatggcaca cacgagagtc ggtatctgga agctcacctc gggaagcgac
     7741 cgggacgaac tggcacaagt gtttcgcgag cagatcgtgc cggagttcca gcagcaaccc
     7801 ggctttatca gctacctggc cgtgggatcc ggccgtagcc aggccgccgg cgtgcagacc
     7861 tgggagacgc cggagcaggc ggttcaggca ggcgccagga ccggcgatcg ggtgcggcag
     7921 cagatcggtg gccaggcgac cctggagagc tggttccacg gaccggtcgc gatcgacatc
     7981 gcgcgccagc cgctggacaa ggcgcgctac gcccgcatcg gcatctggga catcgcgccg
     8041 agcgccaacc ggcaggagct gaccgaaaag gcccgtgaag cgctgatccc catgctgcag
     8101 caggcgagcg gcttggtctc ctaccgtgcg atcgagggga gcgaaggacg cctggtggta
     8161 gtgcattgct gggaatcgga ggaggcctcc gagcggggta tgcaatcgac cggccagtgg
     8221 ctgcaagaga cgctcgcgaa ccagctggcc ctcgtcgccc gctacgacgg cccggtcctg
     8281 ctcgccgcgt ccgctaaaca gggcggctga cggcttgcgt cgttgctgcg tgaggacggg
     8341 tgcttcgact gcgcttagta gtccacccgc cgtcatttgt ccgcttgaca ccgcggcgct
     8401 agactcgcgt gcgacggccg gaagcagacg tgcggagggg gacgatgagc cagcaacacg
     8461 tgatccgctt gacgcccgcg gaacgagcgg agctgcagca attgatccgg gccggggtgg
     8521 cgccagcccg gcaactgacg cgggcgcgca tcgtgctcaa agccgaccg
//
LOCUS       DASUBI010000375         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_569788, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000375 DASUBI010000000
VERSION     DASUBI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569788"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFU72_07305"
     CDS             complement(<1..773)
                     /locus_tag="VFU72_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423327.1"
                     /translation="MPPPGQRLAEVEEAEGHDAVAPPQAPPSNGRERIRHGINHTFSA
                     LAVYNFRILWFGMLFTMAAFQINIVSRSWLAYSISGSGAVLGIVALARGIPQFLLAPY
                     GGVAADRFDKRTLLIVAQLLMAVLSATIAVLVQTGLITIWQLVVIGFFQGVIFPFLMP
                     TRTAYISELVDDGRLPNALALDSTGRNLNRVVAPTLAGFLIALSPAMAFWAVTGFFLL
                     SAAMLLRLPKPTPKPFRSSGTFSDMLFGFRYIAARPNLVA"
     gene            complement(1204..2022)
                     /locus_tag="VFU72_07310"
     CDS             complement(1204..2022)
                     /locus_tag="VFU72_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5423328.1"
                     /translation="MPMIDVNGVALAYERLGEAGPPLVLLHGSWGDHYNFNRVAPLLA
                     GSFRLLSYDRRGHSASQRPDEPVTMADHAADLAALLEALDFAPAYLAGISKGGAVALN
                     LAARRPELIRGLAAHEPPLLDLLAGDPALAALEQETRDRLDAVAALLARGEWARGAEF
                     FVEEVALGRGSWARLSEGARRTFVDNARTFLDESRDPLGYGVDLAALARFAAPVLLTR
                     GDQSPPYFAPVVDRLLATLPRAHVHTFTGAGHVPHASQPEAYAATVASFLRSVA"
     gene            complement(2302..2742)
                     /locus_tag="VFU72_07315"
     CDS             complement(2302..2742)
                     /locus_tag="VFU72_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5423329.1"
                     /translation="MARSLPDGREAAFIAAQRVAHLATAGAHGEPSLVPVCYAWDGAR
                     FFTPIDEKPKRRDRPLRRVRNVEATGRAALVFDHYEEDWDRLGWVLVHGAAEMLAPGA
                     PGHTEAVALLRARYPQYRAMALEAAPLIAITPEQVTSWGRLSVG"
     gene            2814..3101
                     /locus_tag="VFU72_07320"
     CDS             2814..3101
                     /locus_tag="VFU72_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5423330.1"
                     /translation="MKTIAMPSTDERVVTMLRAVANPVRYRILQILAERGECQCGPIS
                     DELPIAQSTLSQHLKVLREAGLIRGEVAGPAVCYCRDEAAIGWLKTQLRAL"
     gene            3110..>3456
                     /locus_tag="VFU72_07325"
     CDS             3110..>3456
                     /locus_tag="VFU72_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5423331.1"
                     /translation="MPEEIDQQAKKTVLRLFTYGLYVVTTRDGETPGSFTANWLSQAS
                     FDPPLVMVSVEADSHSLPLIRASGRFAVNVLESGQRELAGHFGKKARHVGNKLEGIPH
                     HLSASGLPVLDAAL"
BASE COUNT          556 a         1176 c         1243 g          481 t
ORIGIN      
        1 gccaccaggt tcggccgcgc ggcgatgtag cggaagccga agagcatgtc ggagaaggtg
       61 ccgctgctgc ggaagggctt cggcgtcggc ttgggcagcc tgagcagcat cgccgccgag
      121 agcaggaaga agcccgttac cgcccagaag gccatcgccg ggctgagcgc gatcaggaag
      181 ccggccaggg tcggcgccac cacacggttg aggttgcggc cggtcgagtc gagcgccagc
      241 gcgttcggca gccgcccgtc atcgaccagc tccgagatgt aggcggtgcg cgtcggcatc
      301 aggaagggga agatcacgcc ctggaagaaa ccgatgacca ccagctgcca gatggtgatc
      361 aacccggtct gcaccaacac ggcgatggtg gccgagagca ccgccatcaa gagctgcgcc
      421 acgatcagca gggtgcgctt gtcgaaacgg tcggcggcca cgccgccgta gggcgccagc
      481 aggaactgcg ggatgccgcg cgccagcgcc acgatgccga gcacggcgcc ggagccggag
      541 atgctgtagg ccagccagga gcgcgagacg atgttgatct ggaaggccgc catggtgaag
      601 agcatgccga accagaggat gcggaagttg tagacggcca gcgccgagaa ggtatggttg
      661 atcccatgcc ggatgcgttc gcgcccattg gagggcggtg cctggggcgg cgcgaccgcg
      721 tcgtggccct cggcctcctc gacctcggcc agacgctggc ccggaggtgg catggtgttc
      781 gcttggctcc cgtctatcgg tcttatatcg tctaacgacc agcggcaagc ataacagatc
      841 gtgccacgag gcgcgacgca ccttccgttc cagaatggcg ccggccacgg acgagccgcg
      901 gaggaaatgg ggcggatgta tcaccatata cgtaagtatg cgactgtgct ggtggaatcc
      961 ggcgatacag tgggcggttt atgcgaagac ggattgattg ccgccagggg cagggaggcg
     1021 caacgcccgg gcaacgcgcc tggcctgcga ggaggttggg tgtcgcgtgc ggcacggatc
     1081 cttcgacacg ctcaggatga cagggtaagg gtggggccta tcggatgagg tcaatctctc
     1141 tcgtctgtcc gaccttaacc ctgtcatccc gaccgaagtg gagggatccg ttctcgaggg
     1201 cgctcaggcc acggaccgga ggaaggaggc tacggtcgcg gcgtaggcct ccggctgact
     1261 ggcgtggggc acgtggccgg cgccggtgaa ggtgtgcacg tgggcgcgcg gcagcgtcgc
     1321 cagcagccgg tcgaccaccg gcgcgaagta gggcgggctc tggtcgccgc gggtcagcag
     1381 caccggcgcg gcgaagcgcg ccagggcggc cagatcgacc ccgtagccga gcgggtcgcg
     1441 cgactcgtcg aggaaggtgc gcgcgttgtc gacgaaggtg cggcgcgcgc cctcggagag
     1501 ccgcgcccag cttccccgcc ccagcgccac ctcctccacg aagaattcag cgccgcgcgc
     1561 ccactccccc cgcgccagca gcgcggccac cgcgtcgagc cgatctcggg tctcctgctc
     1621 gagcgcggcg agggcgggat cgccggccag caggtcgagc agcggcggct cgtgcgccgc
     1681 caggccgcgg atcagctccg ggcggcgcgc ggccagattg agcgccaccg cgccgccctt
     1741 ggagatgccg gccaggtagg ccggggcgaa gtcgagcgcc tccaggagcg ccgccaggtc
     1801 ggccgcgtgg tcggccatcg tcaccggctc gtcgggccgc tggctggcgc tgtggccgcg
     1861 acggtcgtag ctgagcaggc ggaaggagcc ggccagcagc ggcgccacgc ggttgaagtt
     1921 gtagtggtcg ccccaggagc cgtgcagcag caccagcggc ggcccggcct cgcccagccg
     1981 ctcgtaggcg agcgcgaccc cgttgacgtc gatcatgggc atggtctgga ctcccttggt
     2041 gttctgggcg tgggttggtg gccgtcgaga gggcattgtg cggggtggag cacggatccc
     2101 tccactgcgg tcgggatgac cgggaagggg aggcggcctc ccggacgaga ccgatcatcg
     2161 tccgagggtc atcgcccaca gcccattctc gtcgtcagcc cgacactccc cctgtcatcc
     2221 cgaccgcagt ggagggatcc gtcttcgacg gcagccaatc ctctcgacgg gcagcgtccc
     2281 cgcgcagtct agcgcgccgg ctcaaccaac ggacagcctg ccccagctcg tcacctgctc
     2341 gggggtgatc gcgatcagcg gggcggcctc cagcgccatg gcgcggtact gcgggtagcg
     2401 cgcgcgcagg agcgccacgg cttcggtgtg acccggcgca ccgggcgcca gcatctccgc
     2461 cgcgccgtgc accagcaccc agcccagccg gtcccagtcc tcctcgtagt ggtcgaagac
     2521 cagcgccgcg cgcccggtcg cctccacgtt gcgcacacgc cgcagcgggc ggtcgcgccg
     2581 cttcggcttc tcgtcgatcg gcgtgaagaa gcgcgccccg tcccaggcgt agcagaccgg
     2641 caccagcgag ggctcgccgt gcgcgccggc ggtcgccagg tgcgccacgc gctgcgccgc
     2701 gatgaaggcc gcctcgcgcc cgtccggcag gctccgcgcc atgttcactc ctttcgcgca
     2761 ttgacagcgg cggccagata tcgtaaaaac acgaagacga tgagggggcc gccatgaaga
     2821 cgattgccat gccaagcacc gacgagcgcg tcgtgacgat gctgcgcgcc gtcgccaacc
     2881 cggtgcgcta ccgtattctc cagattctgg ccgagcgcgg cgagtgccag tgcgggccga
     2941 tcagcgacga gctgccgatc gcgcagtcga cgctgtcgca gcatctcaag gtgttgcgcg
     3001 aggcgggcct gatccgcggc gaggtcgccg ggccggcggt ctgctactgt cgcgacgagg
     3061 ccgccatcgg ctggctcaag acccagctcc gcgcgctcta ggaggaatca tgcccgagga
     3121 gatcgaccag caggccaaga agacggtcct gcggctcttc acctacggcc tctacgtcgt
     3181 caccaccagg gacggcgaga cgcccggctc cttcaccgcc aactggctca gccaggcgtc
     3241 gttcgatccc ccgctggtga tggtctcggt cgaggccgac tcccactcgc tgccgctcat
     3301 ccgcgccagc gggcgcttcg cggtcaacgt gctggagtcg ggccagcgcg agctggccgg
     3361 gcacttcggc aagaaggcgc ggcatgtcgg caacaagctg gagggcatcc cccaccacct
     3421 cagcgccagc ggcctgccgg tgctcgacgc cgccct
//
LOCUS       DASUBI010000376         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_561668, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000376 DASUBI010000000
VERSION     DASUBI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561668"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFU72_07330"
     CDS             <1..927
                     /locus_tag="VFU72_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5423332.1"
                     /translation="ALQGVSFTVELGKVFGLLGPNGAGKSTIVKILTTLSRPDSGRAL
                     VAGHDVAREPESVRRAIGYVSQQAAVDLEATGAENLLLQGQLYGLSGAELHRRGGELL
                     ARFGLHEAAAHLARTYSGGMQRKLDVAMGLIHRPRVLFLDEPTTGLDPEARAEMWREI
                     TRLAHDEGLAILLTTHYLDEADHLAERVAIIDRGRIVAAGTPEQLKSELRGDAILLEL
                     AELAPEGAVRAALSRLDGEIREVALAGRELRARADQGATAAAAVLQALEGHGIAVASV
                     AVARPSLDDVYLRYAGRAFVAAQGKSEGGDNR"
     gene            924..1700
                     /locus_tag="VFU72_07335"
     CDS             924..1700
                     /locus_tag="VFU72_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423333.1"
                     /translation="MSAMVLASSHLSMRRLRAMARQPAFIAVTLVQPVIWLLLFGQLF
                     KRVVEIPGFTGDSYITYLTPGVVVMTALFSSGWSGMSLIVEMQRGVLNRFLVTPAPRA
                     ALTVGNVIYQGVLVLIQSAIIVALGLALGARFHGGPLALLAFAVAIVLLTAAFTAFSD
                     AIALVARTEESVIALSTFLVLPLSFLSSAFMPQTLLPGWMQVVARFNPVNWTVEVGRE
                     TLSAGTDWTLIATRLGLLLLLAVVCTGLATRAFRAYRRSI"
     gene            2022..>2368
                     /locus_tag="VFU72_07340"
     CDS             2022..>2368
                     /locus_tag="VFU72_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423334.1"
                     /translation="MAGASTQTTTSAGPRRPRFAALHSRNFRILWFGLIFSNAGAQVQ
                     MVAESWLILQLTNSPLALGIMSLCFGVPMIVLPMLGGALADRLDRITILKFTQSEEIV
                     VPLALSLIIAGGHL"
BASE COUNT          324 a          821 c          814 g          409 t
ORIGIN      
        1 gcccttcagg gcgtgagctt cactgtcgag ctgggcaaag tcttcggctt gctcgggccg
       61 aacggcgccg gcaagtccac catcgtcaag attctgacga cgctctcgcg gcccgactcc
      121 ggccgcgcgc tggtcgccgg ccacgacgtg gcgcgcgagc cggagtccgt gcgccgcgcg
      181 atcggctacg tctcacagca ggccgcggtc gatctggagg cgaccggcgc cgagaacctg
      241 ctgctccagg ggcagctcta cgggctctcc ggcgccgagc ttcaccgccg cggcggtgag
      301 ctgctggcgc gctttgggct gcacgaggcg gcggcgcacc tggcgcgcac ctactccggc
      361 ggcatgcagc gcaagctcga cgtcgccatg ggactgatcc accggccgcg cgtcctcttc
      421 ctcgacgagc cgaccaccgg gctggacccc gaggcgcggg ccgaaatgtg gcgtgagatc
      481 acacgactgg cccacgatga ggggttggcg atcctgctga cgacccacta ccttgacgag
      541 gccgaccacc tggccgagcg cgtggcgatc atcgaccgcg gccgcatcgt ggccgccggc
      601 acacccgagc agctcaaaag cgagctgcgc ggcgacgcca tcctgctgga gctggccgag
      661 ctggcaccgg agggcgctgt gcgcgccgcg ctgagccggc tcgacggcga gatccgcgag
      721 gtcgcgctgg cggggcggga gctgcgggcg cgggccgacc agggcgcgac cgcggcggcc
      781 gccgtgcttc aggcgctcga agggcacggc atcgccgtcg catcagtcgc ggtcgcgcgc
      841 ccgtcgctcg acgatgtcta tctgcgctac gccggccgcg ccttcgtggc ggcacagggg
      901 aagagtgagg ggggagacaa ccgatgagcg cgatggtcct ggcgtcgagc cacctgagca
      961 tgcgccggtt gcgggcgatg gctcgccagc ctgcctttat cgcggtgacg ctggtgcagc
     1021 cggtgatctg gctgctcctc ttcggccagc tcttcaagcg cgtggtcgag atccccggct
     1081 tcaccggaga ctcctacatc acctacctaa cgccgggcgt ggtggtgatg accgcgctct
     1141 tctccagcgg ttggtcaggc atgtccttga tcgtcgagat gcagcgcggc gtgctcaatc
     1201 gcttcctggt gacgcccgcg ccgcgcgccg cgctcaccgt cggcaacgtc atctaccagg
     1261 gtgtgctggt gctgattcag tcggcgatca tcgtggcgct cgggctggcc ctcggcgcgc
     1321 gcttccacgg cggcccgctg gcgctgctgg ccttcgccgt ggcgatcgtg ctgctgaccg
     1381 cggctttcac cgccttctcg gacgcgatcg cgctggtcgc gcgcaccgag gagtcggtga
     1441 tcgcgctctc gaccttcctg gtgctgccac tctccttcct ctcgtcggca ttcatgccgc
     1501 agacgctctt gccgggctgg atgcaggtcg tggcgcggtt caacccggtc aactggacag
     1561 tcgaggtcgg gcgagagacg ctgagcgcgg ggacggattg gaccctgatc gccacccgcc
     1621 tggggctcct gctgttgctg gcggtcgtct gcaccggtct ggcgacgcgc gcgtttcggg
     1681 cctatcgacg ctcgatctag gaaggccgat tcgtcaagcg ggcgcctgcc tgatgaccgc
     1741 gttggtcgtt gtgtgaggac ggctccatcg acaagctcag gatgacaggg gaggggatga
     1801 ggctgacgac gacgtgtgtc gccctcgtac gcctctatcc cccatgtatg acccctgtgc
     1861 gtgtcgaagg ttccgtgccc cacgcggcac ccaacgtcga ctgagggcaa ctgctcgccc
     1921 ttcggcctat ccgaagggac agggcatttc gctggcacgg ccgcgagcgg tcgtgtcaga
     1981 ctacacctat cgagcgtggc gagccggtct gggagcaacg agtggcgggc gcatcgaccc
     2041 aaacgaccac cagcgcgggc ccgcgccgcc cgcgcttcgc cgcgctgcac tcgcgcaact
     2101 tccgtatcct ttggttcggc ctgatcttct ccaacgccgg cgcccaggtg cagatggtgg
     2161 ccgagtcgtg gctgatcctg caactgacca actcgccgct ggcgctcggc atcatgagcc
     2221 tctgcttcgg ggtgccgatg atcgtgctgc cgatgctcgg cggcgccctg gccgaccggc
     2281 tcgaccgcat caccattctc aagttcaccc agtctgagga gatcgtcgtc ccgcttgcac
     2341 tcagtctgat catcgccggc gggcacct
//
LOCUS       DASUBI010000377         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_709811, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000377 DASUBI010000000
VERSION     DASUBI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709811"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFU72_07345"
     CDS             complement(<1..391)
                     /locus_tag="VFU72_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423335.1"
                     /translation="MIETLIEFALLVGLTAAISTWAHAAQQRQRLRLALLILLGVIAV
                     PLLIGGVIALLALGLRNAGAWLALALGLALGLPLLRPVRVALAWVTPMSADSAPDLVG
                     LAILAAVAVVSGWSSLTAGPHLGSAASP"
     gene            508..1365
                     /locus_tag="VFU72_07350"
     CDS             508..1365
                     /locus_tag="VFU72_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5423336.1"
                     /translation="MAQRKGGLGRGLSALIQSQGELSGVQEIEVAAVSPNPYQPRESF
                     DDEALQGLAASIRELGVLQPVIVSRNQDGAAFQLLAGERRWRAARLAGLRTIPAIIRE
                     TSPRQMLEVALIENVQRADLSVLEEATAYRQLIDDFGLTQAEVAERAGKSRVAVTNAL
                     RVLAAPEEIRAALVGRQITEGHARALLGLAHAAEQVAALELVIARNLSVRQTETLVRD
                     WRERPPARPHPTEPAAPQRRLAEAFQRALGTKVDLQRGRKGGRLVIHYYSDEELDGLY
                     RRIVGEDEV"
     gene            complement(1522..>2602)
                     /locus_tag="VFU72_07355"
     CDS             complement(1522..>2602)
                     /locus_tag="VFU72_07355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5423337.1"
                     /translation="EGMPLEEMTRLIRESNLACPVCGQRDWTDVRQFNMMFQTTIGPV
                     ESSGTAAFLRPETAQAIFLDFRNVLQTSRQKLPFGIAQIGKAFRNEITPGNFIFRLLE
                     FEQMEIEYFIRPEGWEEHFEQWLRLVADFYAWIGLRPDRLRLREHSPEELSHYSRRTV
                     DYEYKFPFGWKELSGQAYRTDFDLRRHMEFSGVDLSYFDQATNERFLPHVIEPTVGVE
                     RLMLTLLLDAYDEEETVDVNGKPYTRVVLRLHPRVAPYQVAVLPLMRKDELVAKAREV
                     ETLLRQVVRVDYDETQSIGRRYRRQDEIGTPYCVTIDYETLDDAAVTLRDRETMEQAR
                     IPIAELAGTVAARIAAWPEATAVRP"
BASE COUNT          419 a          934 c          884 g          365 t
ORIGIN      
        1 tcggcgaggc ggccgacccc aggtgcgggc cggcggtcag tgagctccag cccgaaacca
       61 ccgccacggc cgccaggatc gccaggccca ccaaatccgg cgccgagtca gccgacatcg
      121 gcgtgaccca ggccagcgcg acccggaccg gccgcagcag cggaagcccc agtgccagcc
      181 ccaacgccaa cgccaaccag gcgccggcgt tgcgcagccc gagcgcgagc agcgcgatca
      241 cgccgccgat cagcagcggc acggcgatca ccccgaggag gatcaagagc gccaggcgca
      301 accgttggcg ctgctgcgcc gcgtgcgccc aggtcgaaat ggctgccgtc aggccaacca
      361 gcagcgcgaa ctcaatgagc gtctcgatca acgacccctc cccagcaaga ctcgaacccg
      421 agccgcgcgc cacatgctac actcctgcca ccggggcgaa gcgcgagggt gggcgcgagg
      481 acgcgcgtgg gtacgaggga gtcgagcatg gcacagcgca agggagggct cggccgtggt
      541 ctgagcgcgc tgatccagtc gcagggtgag ctgagcggtg tgcaggagat cgaggtcgcg
      601 gcggtcagcc cgaaccccta ccagcctcgc gagtccttcg acgacgaagc cctgcagggc
      661 ctggccgcct cgatccgcga acttggcgtc ttgcagccgg taattgtcag ccggaatcag
      721 gacggcgccg cctttcaact cctcgccggc gagcgccgct ggcgagccgc ccggctcgcg
      781 ggcctccgca cgatccccgc tattatccgt gaaaccagcc cccggcagat gctcgaagtg
      841 gccctaatcg agaacgtgca gcgggcggac ctctccgtat tggaagaagc caccgcctac
      901 cgccagctga tcgacgactt cgggctgacg caggccgagg tcgccgagcg cgccggtaag
      961 agccgggtag ccgtcaccaa cgcgttgcgg gtcctggccg cgccggagga gatcagggcg
     1021 gccctggtcg gccgccagat caccgagggc cacgcccgcg cgctgctcgg gctggcgcac
     1081 gccgccgagc aggtcgccgc gctggagctg gtgatcgcgc gcaacctctc agtgcggcag
     1141 accgaaacgc tggtgcgcga ttggcgcgaa cggcctcccg cgcggccgca ccccaccgaa
     1201 ccggccgcgc cgcagcgccg gctggccgag gccttccagc gcgcgctcgg caccaaggtc
     1261 gatctccagc gcggccgcaa gggcgggcga ctcgtgatcc actactactc cgacgaagag
     1321 ctcgacggtc tctaccgccg catcgtcggc gaggacgagg tctagccctc accccctccc
     1381 ctctcccgtg cgcgtctccg cacggagagg ggcgctgccc atcgatacag ctcgccaatg
     1441 agaagtgtgg cctattcttg tcgttcgcca cctcccctcg cccccgcagg ggagaggggc
     1501 agggggtgag ggccgtcgtg cctacggcct gaccgccgtc gcctctggcc aggccgcgat
     1561 gcgggcagcc accgtgccgg ccagctcggc gatcgggatg cgcgcctgct ccatcgtctc
     1621 gcggtcgcgc agcgtcaccg ccgcgtcgtc cagcgtctcg tagtcgatgg tgacgcagta
     1681 gggcgtgccg atctcgtcct ggcggcggta gcgccgcccg atgctctgcg tctcgtcgta
     1741 gtccacgcgc accacctggc gcaggagcgt ctcgacctcg cgcgccttgg cgaccagctc
     1801 gtccttgcgc atcagcggca ggaccgcgac ctgatagggc gccacgcgcg gatgcagccg
     1861 cagcaccacc cgcgtgtagg gcttgccgtt gacgtcgacc gtctcctcct cgtcgtaggc
     1921 gtccagcagc agcgtcagca tcaggcgctc gacgccgacc gtcggctcga tcacgtgggg
     1981 gaggaagcgc tcgttcgtcg cctgatcgaa gtagctgagg tcgaccccgc tgaactccat
     2041 gtgccggcgc aggtcgaagt cggtgcggta ggcctggccc gacagctcct tccacccgaa
     2101 ggggaacttg tactcgtagt ccaccgtgcg ccgcgagtag tgcgagagct cttcgggcga
     2161 gtgctcgcgc aggcgcagcc ggtccgggcg cagcccgatc caggcgtaga agtcggccac
     2221 caggcgcagc cactgctcga agtgctcctc ccagccctcg ggccggatga agtactcgat
     2281 ctccatctgt tcgaactcga gcaggcggaa gatgaagttg ccgggggtga tctcgttgcg
     2341 gaacgccttg ccaatctggg cgatgccgaa ggggagcttc tgccggctcg tctgcagcac
     2401 gttgcggaag tcgaggaaga tggcctgggc cgtctcgggt cgcaggaagg ccgccgtgcc
     2461 ggaggactcc acgggcccga tcgtggtctg gaacatcatg ttgaactgac gcacgtcggt
     2521 ccagtcgcgc tggccacaga ccgggcaggc gaggttgctc tcccggatca ggcgcgtcat
     2581 ctcctccagc ggcatgccct cc
//
LOCUS       DASUBI010000378         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_341255, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000378 DASUBI010000000
VERSION     DASUBI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341255"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFU72_07360"
     CDS             <1..342
                     /locus_tag="VFU72_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5423338.1"
                     /translation="ADLLNYNVLCQGGRITGVLDWGSAFYGDPLYDVVWCRFYEPWYP
                     EFKRITLPERLLADFRADPRANTTDIDARVRCYELHIGLDSIAYNAFKQDWAHAKEAA
                     AYTLALTAPGR"
     gene            complement(367..654)
                     /locus_tag="VFU72_07365"
     CDS             complement(367..654)
                     /locus_tag="VFU72_07365"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU5423339.1"
                     /translation="MPMFVILGHFTDQGAKNVKELLPAFQKNRANGEKLGIKLHGWYL
                     TQGRYDFIVLVEAPDEKVMLQQTLAVAGHGLTRSETLRAYPIEEVQQMLQG"
     gene            972..2060
                     /gene="msrA"
                     /locus_tag="VFU72_07370"
     CDS             972..2060
                     /gene="msrA"
                     /locus_tag="VFU72_07370"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5423340.1"
                     /translation="MFFNRGKSKPVSAEDALPGREQPSYDVPERHEVLGTSLKPPFPA
                     GLETAQFALGCFWGAERLFWELPGVYSTASGYAGGATPNPTYREVCSGKTGHAESVQV
                     VFDPKQISYAELLKTFFEAHDPTQGMRQGNDIGTQYRSAVYYLNDEQRRLAEAARDAY
                     NAELRAARRGEITTEIAPAGPFYYAEDYHQQYLHKVPDGYCGLAGTGVTCPLPAAVSG
                     DADPELLPVLGSDEEWRQRLNKEQYNVLRRAGTERPFTGEYVDTETDGLYRCAACGNL
                     LFDSGTKFHSDCGWPSFTEAVPGAVEYLEDRSYGMTRTEVRCARCHSHLGHVFDDGPV
                     DRGGQRYCMNSVSLKLKPRLQVRLPQTG"
     gene            2195..3025
                     /locus_tag="VFU72_07375"
     CDS             2195..3025
                     /locus_tag="VFU72_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEU5423341.1"
                     /translation="MERPDNGHWADAQLAEVSEEPYYLPIADEVTIFEAAYARRLPVM
                     LKGPTGVGKTRFLRHMAHRLGRPLVTVACHDDLTTSDLLGRYLIKGDETVWVDGPLTY
                     ALRIGAICYLDEIVEARKDTTVIIHPLTDDRRRLRIEKTDELIIAPPEFLLVVSYNPG
                     YQSLLKNLKPSTRQRFVALDFDFPPPELETEIVAHESGVDPATASQLVRLGNLIRNLS
                     DSGLDEPASTRLLIYAGQLIGGGIGPRRACELAIAAPITDDQTVQSAVLDLVATVFPG
                     "
BASE COUNT          514 a         1131 c         1044 g          498 t
ORIGIN      
        1 gccgacctgc tcaactacaa cgtcctctgc cagggtggcc gcatcaccgg ggtgctcgac
       61 tggggctccg cgttctacgg cgacccgctc tacgacgtcg tctggtgcag gttctatgag
      121 ccgtggtatc cggagttcaa acgcatcacg ctgccggagc gcctgctggc ggactttcgc
      181 gccgacccgc gcgccaacac aaccgatatc gacgcacggg tccgctgcta cgagctgcat
      241 atcgggctcg actcaatcgc ctacaacgcc ttcaagcagg actgggcaca tgccaaagag
      301 gcggccgcct atacgctggc gctgaccgcg cccgggcgtt gagaccggct gagcgggtcc
      361 gggcgatcag ccctgcagca tctgctggac ctcctcgatc ggataggcac gcagggtttc
      421 gctgcgggtc aggccgtggc cggccacggc cagcgtctgc tgcagcatga ccttctcatc
      481 cggcgcttcg acgagcacga tgaagtcata ccggccctgg gtcaggtacc aaccgtggag
      541 cttgatgccc agcttctctc cgttggcccg gttcttctga aacgccggga ggagttcctt
      601 gacgttcttg gcaccctggt cggtaaagtg gccgaggatc acgaacatcg gcatcgggcc
      661 gctcccttct ggtgtcggtg cggtcggcag cgttccgtgg ccactctcgc agatgtcgga
      721 gcggctgtca acgagaattg gagcgagggg acgaggatag tgtcgcattc cgcgactttt
      781 tgtcggaata tccaagcatg attggatcgc gtcgcgtgat acgcttcagg cgaacaggtg
      841 cgcgagtcgg ctgtgggccg gaagaatcgg agccctccgg ctgttggtta catcacaacc
      901 cgtcgtcgcg gctggctcgc cgcgcggcag cgccctgagg acacaatcca accccacccg
      961 gaggtgcatc tatgttcttc aaccgcggca agtcgaagcc tgtttccgcc gaggacgccc
     1021 tgcccgggcg cgagcagccg tcctacgacg tgcccgagcg gcacgaggtg ctgggcacct
     1081 cgctcaagcc gcccttcccc gcggggctgg agacggcgca gttcgcgctc ggctgcttct
     1141 ggggcgccga gcggctcttc tgggagctcc ctggcgtcta cagcacggcc tccggctacg
     1201 ccggcggcgc cacgcccaat ccgacctacc gcgaggtctg ctcgggcaag accggccacg
     1261 ccgagtcggt gcaggtcgtc ttcgacccca agcaaatctc ctacgccgag ctgctgaaga
     1321 cgttcttcga ggcccacgac ccgacccagg ggatgcgcca gggcaacgac atcggcacgc
     1381 agtaccgctc ggccgtctac tatctgaatg acgagcagcg gcggctggcc gaggcggcgc
     1441 gcgacgccta caacgcggag ctgcgcgcgg cccggcgcgg cgagatcacc accgagatcg
     1501 cccccgctgg ccccttctac tacgccgagg actaccacca gcagtacctg cacaaggtgc
     1561 cggacggcta ctgcgggttg gcaggcaccg gcgtcacctg cccgctgccg gccgcggtca
     1621 gcggcgacgc cgacccggag ctgctgccgg ttctcggcag cgatgaagag tggcgccagc
     1681 ggctgaacaa ggagcagtac aacgtgctgc gccgggccgg gaccgagcgc cccttcaccg
     1741 gcgagtacgt cgacaccgag accgacggcc tctaccgctg cgccgcctgc ggcaacctcc
     1801 tcttcgacag cggcaccaag ttccactccg actgcggctg gccgagcttc accgaggccg
     1861 ttccgggcgc ggtcgagtac ctggaggacc gctcctacgg catgacccgc accgaggtgc
     1921 gctgcgcccg ctgccactcc cacctgggcc acgtcttcga cgacgggccg gtggaccggg
     1981 gcggccagcg ctactgcatg aacagcgtgt cgctcaagct gaagccgcgg ctccaggtgc
     2041 gcctgccgca gaccggctag ctcgccggga tcggacacgg tccacgtctc atccttccca
     2101 ggctgtcgcg tgtcgcaccg gctccgagat acggccgggg ccggtagact tgggagtggc
     2161 acgacgagga gagtctgggg aggatgcagg cgagatggag cgaccggata acgggcactg
     2221 ggccgacgcg caactggcgg aggtcagcga ggagccctac tacctgccga tcgccgacga
     2281 ggtgacgatc ttcgaggcgg cctacgcccg ccggctgccg gtgatgctca aggggccgac
     2341 cggggtgggc aagacgcgct tcctgcgcca catggcccac cggctgggcc gcccgctggt
     2401 cactgtggcc tgccacgacg acctgaccac cagcgacctg ctggggcgct acctgattaa
     2461 aggcgacgag acggtctggg tcgacggccc gctgacctac gccttgcgca tcggcgccat
     2521 ctgctacctg gatgagatcg tcgaggcgcg caaggacacc accgtcatca tccacccgct
     2581 gaccgacgac cggcggcggc tgcgcatcga gaagaccgac gagctgatca tcgccccgcc
     2641 cgagtttctc ctggtcgtct cctacaaccc cggctaccag agcctgctca agaacctcaa
     2701 gccgagcacc cgccagcgct tcgtggccct cgacttcgac ttcccgccgc ccgagctgga
     2761 gaccgagatc gtggcgcacg agagcggcgt cgacccggcc accgccagcc agctggtgcg
     2821 gctgggcaac ctgatccgca acctgagcga ctcggggctg gatgagccgg ccagcacgcg
     2881 cctcctgatc tacgccggac agttgatcgg cggcggcatc ggcccccggc gcgcctgcga
     2941 actggcgatc gcggcgccga tcaccgacga ccagacggtg cagagcgcgg tgctcgatct
     3001 ggtcgccacc gtcttcccag gctagccgtg ccgcgttggc tctcccgcct cctcgccgag
     3061 ccgcgcccat cgcggcgggc atccgttgac gaggtgccgc gctttagctt ccccttcagc
     3121 gaggtcgagg gcacgctggc cctctacgtc accgccctct cggacggcgc gctgcgtgtg
     3181 gccgcca
//
LOCUS       DASUBI010000379         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1307959, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000379 DASUBI010000000
VERSION     DASUBI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307959"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFU72_07380"
     CDS             <1..221
                     /locus_tag="VFU72_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HEU5423342.1"
                     /translation="QEEHRGKLAYFAGIDGVRFKRQVVPGDTLRLEVELGRLRRGVGV
                     GNAVATVDGELAARGELMFALVDAAREA"
     gene            211..1365
                     /locus_tag="VFU72_07385"
     CDS             211..1365
                     /locus_tag="VFU72_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5423343.1"
                     /translation="MRPDRPWRTRAIERLARAADRVSRARHPASSLDSGQARRILVVK
                     PCCLGDMLMATPALRALKHHFPGARIQVLTTDWCVPALDNNPHVAGTLPYPPHPTPVR
                     LLALAQRLRQERFDVGVSLDRSPLVNGLLLLAGIPERAGIDSRGRGVGLTRRASPKPE
                     QHESDLFLSVAAALGVPPCGEQPEFHPSLEARQQAERVIGGAQRPLVVIHPGGALNPG
                     SAMLSKRWPAIAFGELASLLVQREGATIVLVGALSDRDAVQVAADFTDGPVIDLGGQL
                     SIPELAAVVERADLYIGNDSGASHLAAAVGTPTVTIFGPTSPYQYRPLGPCAQVCAPQ
                     GSWNPGLVGLDLRGRRAQLDLAADIRDVSVDEVYTACHDVLAAPREMQRP"
     gene            1362..2501
                     /locus_tag="VFU72_07390"
     CDS             1362..2501
                     /locus_tag="VFU72_07390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5423344.1"
                     /translation="MIRPLARALLTRGLTTLAPPVQPGNSRSVLLIRPDHLGDLLFLT
                     PGLRRLRAALPGWRLVLACGPWGAPVMAGNPDIDELIELPFPGFTRAPSPHPLQPYQL
                     ARVAAAQLRARDFAAAVVLRDDHWWGGLLALLAGIPRRIGASDPAVASLLTQRVPVAG
                     HSVARNTALLDAAARLLGGTPPDRSPTPERDPLAWTVTEQDRADARRLTAAEPYVAVH
                     PGSGAPVKSWPAARWAELADRLAARKVRVVFTGGEAERWLVETIRARMASDSLNLAGQ
                     TSLRALGAIYADARAVIGVDSGPLHLAVAAGAPTVQLYGPSDPEKFGPWGPVERHRVL
                     HVGLHCSRCGDLSPGRPEGAGCMLALDGECVSRALFELIEPPADG"
     gene            2494..>3361
                     /locus_tag="VFU72_07395"
     CDS             2494..>3361
                     /locus_tag="VFU72_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423345.1"
                     /translation="MAEARALTVGIDASRATIAQKTGTERYSARILEAVLALGTRHRF
                     RLYLNARQPLPLALRAGDRQRLIPFPRLWTHARLSAELAGHPPDALFVPAHVVPLVHP
                     RASGVTIHDLGYLYEPEAHPVARRLALDLTTRWSARAAARVIAISGATRDDLVRRYGV
                     PAEKITVIHHGVDGAFQPPAEAEVARVRERYQLPARYLLSVGTLQPRKNLARLIAAFE
                     RLAAEDEELGLILAGKTGWLAGTIEAALTASPARARITLTGHVPDADLPALYGGAAAF
                     AFPSLYEGFGMPA"
BASE COUNT          414 a         1273 c         1185 g          489 t
ORIGIN      
        1 gtcaagagga gcaccggggc aagctggcct acttcgcggg tatcgacggc gtgcgcttca
       61 agcgtcaggt ggtacccggc gatacgctgc ggctggaggt cgagctgggc cggctgcgcc
      121 ggggcgtcgg cgtcggcaac gcggtggcga cggtcgatgg cgagctggcc gcgcgcggcg
      181 agctgatgtt tgcgctggtc gatgccgctc gtgaggcctg accggccctg gcgcacccgc
      241 gcgatcgagc ggctggcgcg cgcggccgat cgcgtcagcc gcgcccggca ccccgcttcg
      301 tcgctcgact ccggtcaggc gcggcgtatt ctggtcgtca agccctgctg cctgggcgac
      361 atgctgatgg cgacgcccgc gctgcgcgcg ctcaagcatc acttccccgg cgcccgtatc
      421 caggtgttga cgaccgactg gtgcgtgccc gcgctcgaca acaaccccca cgtggccggc
      481 acgctcccct atccgccgca ccccacgccc gtgcgcttgc tggcgctggc gcagcggctg
      541 cgccaggagc gcttcgacgt cggggtcagc ctcgaccgct cgccgctggt caacggccta
      601 ctgctgctgg ccggtatccc cgagcgcgcc gggatcgaca gccgcgggcg aggcgtcggg
      661 ctgacgcgcc gggccagtcc caagccggag cagcacgaga gcgacctgtt tctctcggtc
      721 gccgccgcgc tcggcgtgcc gccctgcggc gagcagccgg agttccaccc ctcgctggag
      781 gcgcgccagc aagccgagcg agtgatcggc ggcgcgcagc ggcccctggt ggtgattcat
      841 cccggcgggg cgctcaatcc cggcagcgcc atgctctcca agcgctggcc ggccatcgcc
      901 ttcggcgagc tggccagcct gctggtgcag cgtgaaggcg ccaccatcgt gctggtcggc
      961 gcgctctccg accgcgacgc ggtgcaggtc gcggccgatt tcaccgatgg accggtcatc
     1021 gatctgggcg ggcagctctc gatccccgag ctggcggcgg tggtcgagcg cgcagacctc
     1081 tacatcggca acgacagcgg cgccagccac ctggccgcgg cggtcggcac cccgaccgtg
     1141 accatcttcg gcccgaccag cccgtaccag taccgcccgc tcggaccctg cgcacaggtc
     1201 tgcgcgcccc agggcagttg gaatcctggc ctggtcgggc tcgacctgcg cggccggcgc
     1261 gctcagctcg atctggcggc ggatatccgc gacgtcagcg tcgacgaggt ttacaccgcc
     1321 tgtcacgacg tgctggccgc gccgcgcgag atgcaacggc cgtgatccgt ccgctcgccc
     1381 gcgcgctgct gacgcgcggg ctcacgacgc ttgccccgcc ggtacagcca gggaactcgc
     1441 gctctgtgct gctgatccgg ccggatcacc tcggcgatct gctcttcctg acacccggcc
     1501 tgcgccgcct tcgcgccgcg ctgcccggct ggcggctcgt gctggcctgc ggcccctggg
     1561 gcgcgccggt catggccggc aaccccgaca tcgacgagct gatcgagctc cccttccccg
     1621 gcttcacccg cgcgcccagt ccgcacccgc ttcagccata tcaactggcg cgcgtggccg
     1681 ccgcgcagct ccgcgcgcgc gacttcgccg ccgcggtcgt gctgcgcgat gaccactggt
     1741 ggggcggcct gctggccctg ctagccggca tcccgcgtcg gatcggcgca agcgatccgg
     1801 ctgtggcctc gctgctgacc cagcgggtgc cggtcgccgg tcacagcgtg gcgcgcaaca
     1861 cagccctgct cgacgccgcc gcgcgtctcc tgggcggcac gccgcccgac aggtcaccga
     1921 cgcccgagcg cgatccgctg gcctggacgg tgacggagca ggaccgggcc gacgcgcggc
     1981 ggctgaccgc cgctgaacca tacgtcgccg tccacccggg ctcgggtgcg ccggtcaagt
     2041 cgtggccagc cgcgcgctgg gccgagctgg ccgatcgcct cgcggcgcgc aaggtccgcg
     2101 tcgtcttcac cggcggcgag gccgaacgct ggctggtcga gacgattcgg gcacgaatgg
     2161 catccgactc gttgaacctc gctgggcaga cctcgctgcg agcgctgggg gcgatctacg
     2221 ccgatgcccg tgcggtgatc ggcgtcgaca gcggcccgct gcacctggcg gtggccgccg
     2281 gggcgccgac agtgcagctc tacggtccca gcgacccgga gaagttcggc ccctggggtc
     2341 cggtggagcg tcaccgggtg ctgcacgtcg ggctgcactg ctcgcgctgc ggtgatctct
     2401 cgccgggtcg gcccgagggc gccggctgca tgctggcgct cgacggcgag tgcgtcagcc
     2461 gggcactctt cgagctgatc gagccgccag ccgatggctg aggcgcgcgc gctgaccgtc
     2521 ggcatcgacg ccagccgggc gacgatcgcc cagaagaccg ggacggagcg ctactcggca
     2581 cgcatcctgg aggccgtgct ggcgcttggc acacgccacc gcttccggct ctacctcaac
     2641 gcgcgccagc cgctgccgct cgccttgcgc gccggcgacc gccagcggct aatccctttc
     2701 ccgcgtctct ggacccacgc gcggctgtcc gcggaactgg cgggtcaccc ccccgatgcc
     2761 ctcttcgtcc cggcgcacgt ggtgccgctc gttcatccgc gcgccagcgg ggtcactatc
     2821 cacgacctcg gctacctcta cgaacccgag gctcacccgg tcgcacggcg actggcgctc
     2881 gatctgacga cgcgctggag cgcccgcgcg gccgcgcgcg tgatcgccat ctccggcgcc
     2941 acgcgcgacg atctggtgcg ccgctacggt gtgccggcgg aaaagattac cgtgatccat
     3001 cacggggtcg atggagcgtt ccagccgccg gcggaggccg aggtcgcgcg ggtgcgagag
     3061 cgctaccagc tcccggcgcg ctacctgctg agcgtcggca cgctgcaacc acgtaagaat
     3121 ctggcgcgcc tgatcgccgc attcgagcgg ctggcggccg aggatgagga gctgggactg
     3181 atactggcgg gcaagaccgg ctggctggcc ggcacgatcg aggccgcgct gaccgctagc
     3241 ccggcgcggg cccgcatcac gctcaccggc cacgtgcccg atgccgacct gccggcgctc
     3301 tacggcggcg ccgcggcctt cgcattccct tcgctgtatg agggcttcgg catgcccgct
     3361 c
//
LOCUS       DASUBI010000380         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_915479, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000380 DASUBI010000000
VERSION     DASUBI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915479"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFU72_07400"
     CDS             complement(<1..1245)
                     /locus_tag="VFU72_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5423346.1"
                     /translation="MADINPRLRAVFDMSVSEVREYSGRHEYDGKIQDLSPDGVGAGL
                     RRLAEAEADSAARIADEHDDAHLRVFTESVKVGYAELAQHRSNPYYHLANMDLACYDR
                     EYAAPEVRASARAEHLAAWPDAVDMAISSLDQVPAPVAKALGGAVRGLAAGVPADGDE
                     PSRAAALAAHARLVSHVDHAAEHGDPDAALGSAALTRLMSSTEGIDLDLGRLAERADT
                     ERDRLTARLAESCAQIDANRPAMDVVHELVRQHPDADGVLDAARIWTTRVIDFTRQKN
                     LVPYQDGECLVALAPQSRRWAMAMMATAAPGESEAPSMYYITPPDPSWPDHEAQEWLE
                     VFSDTTLPGITAHEVAPGHFSHGRAIRRAPTEVRRTLHSGAFIEGWAHYSEELCVEEG
                     FCADDPRFAIGVWLEALVRVTRL"
     gene            complement(1476..>2240)
                     /locus_tag="VFU72_07405"
     CDS             complement(1476..>2240)
                     /locus_tag="VFU72_07405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5423347.1"
                     /translation="GDARSLLRRIGPLPADRAWAIVSQVAAALDAAHRRGLVHRDVKP
                     GNMLLDVSSGLAGTDLTDHVYLTDFGISKQAQSAGLTVTGQILGTLDYAAPEQIEGRD
                     VDGRTDLYSLACSVFELLGGTPPFRRSQGPALIYAHLSTPAPPVTASRPDLPPAADRV
                     IAKALAKAAADRYASCAEFAADLGRALRLIAGRVEAVGHGLLPPVASTKVHPRPKPPL
                     QGPDAAQQVKPVKPAMPPGGLARPPGSEPPTRKREP"
BASE COUNT          314 a          773 c          796 g          357 t
ORIGIN      
        1 gagcctggtc accctgacca gcgcttccag ccaaacgccg atcgcgaagc gcggatcgtc
       61 ggcgcagaac ccttcctcga cgcagagctc ctcgctgtag tgggcccagc cttcgatgaa
      121 ggcgcctgag tgcagagtgc gcctgacctc ggttggcgcc cgtcggatgg ctctgccgtg
      181 cgagaagtgg ccaggtgcga cctcatgcgc ggtgatcccg ggcagcgtcg tgtcgctgaa
      241 cacctcaagc cattcctgcg cctcgtgatc tggccagctc ggatcgggcg gcgtgatgta
      301 gtacatcgac ggcgcctccg actcgcccgg ggccgccgtc gccatcatcg ccatcgccca
      361 gcggcgggac tgcggcgcga gcgcgaccag gcattcaccg tcctggtatg ggaccaggtt
      421 cttctgcctg gtgaagtcga tgacccgcgt ggtccagatc ctcgccgcat caagcacgcc
      481 gtcggcatcg ggatgctggc gcaccagttc gtgcaccacg tccatcgccg gccggttcgc
      541 gtcgatctgc gcgcaactct ccgccagccg cgcggtcagc cggtcgcgct cggtgtcggc
      601 ccgctcggcc agcctgccaa ggtcgagatc gatgccctcg gtgctgctca tcagcctggt
      661 cagggccgcg ctgccgagcg ccgcgtcggg atctccgtgc tcggccgcgt gatcaacgtg
      721 gctgaccagc ctggcgtgcg ccgcgagcgc ggccgctctt gacggctcgt caccgtcagc
      781 cggaacaccg gccgcgaggc cgcgcaccgc gccgccgagc gccttggcca ccggggccgg
      841 aacctggtcg agcgatgaga tcgccatgtc gacggcgtca ggccaggcgg ctaggtgctc
      901 ggcgcgggcg ctcgcgcgca cctccggtgc ggcgtactcc cggtcgtagc aggccaggtc
      961 catgttggca aggtggtaat aggggttgct ccggtgctgc gcaagctcgg cgtagccgac
     1021 cttgacggac tcggtgaaga cgcgcagatg cgcgtcatcg tgctcgtcgg ctatccgcgc
     1081 ggcgctgtct gcttcggctt ccgccagccg gcgaaggccg gcgcccacac catcggggga
     1141 gaggtcctga atcttgccgt cgtattcatg cctgccgctg tactcgcgta cttcggaaac
     1201 cgacatatcg aagacggcgc gcagcctcgg gttgatgtca gccatggccg aaatttacct
     1261 gtgcgggccg ccgcgccact acccggggcc tgcaccggca ggctagtgag agattgagcg
     1321 attcttcgct ggggttagag ggacccgccc ggccagcacg ccgcccggtc cctgttcccg
     1381 gcagccccat cacgcacacc gcaccgggga gagcgacacg gtaagtctcc gtcgtgacaa
     1441 aaagggccac gaacgccggc cggtcaaccg gcaggctagg gctcgcgctt gcgtgtcgga
     1501 ggttcgctgc ctggcggtct ggcgagcccg ccgggcggca tcgccggctt caccggtttg
     1561 acctgctggg cggcgtctgg gccctggagg ggcggcttgg gccttggatg cactttcgtg
     1621 ctggcgactg gcggcagcag cccgtggcca accgcctcga cccggccggc gattagcctg
     1681 agcgcgcggc cgaggtcagc ggcgaactcc gcgcagctcg catagcggtc ggccgcggcc
     1741 ttggccaggg ccttggcgat cacccggtcg gcggctggcg gcaggtcggg cctgctagcg
     1801 gtcacggggg gcgccggcgt cgacagatgc gcgtagataa gcgcgggccc ctggctgcgc
     1861 cggaacggcg gggtgccgcc gagcagttcg aacaccgagc acgcgagcga gtacagatcg
     1921 gtcctgccgt ccacgtcacg gccctcgatc tgctcgggag cggcgtagtc gagcgtgccg
     1981 agtatctggc cggtgaccgt gagaccagcc gactgtgcct gcttgctgat gccgaaatcg
     2041 gtgaggtaga cgtggtcggt caggtcggtg ccagccaggc cgctgctgac gtcgagcagc
     2101 atgttgcccg gtttgacgtc ccggtgcacg agcccgcgcc gatgcgcggc gtcaagcgcg
     2161 gcggctacct gggagacgat ggcccacgcc cggtcggccg gcagcggccc gatgcgccgc
     2221 agcagcgacc gagcgtcgcc
//
LOCUS       DASUBI010000381         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_162916, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000381 DASUBI010000000
VERSION     DASUBI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162916"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFU72_07410"
     CDS             complement(<1..289)
                     /locus_tag="VFU72_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423348.1"
                     /translation="MGEWVRRARVVVVLVAALMLFVALAGQAAAANIDFRVAAFNCLS
                     DPGPLSLAKGAIPSDCTPAAGVAVTVSDTSGAPLANCTTNANGLCILSLTEG"
     gene            741..1811
                     /locus_tag="VFU72_07415"
     CDS             741..1811
                     /locus_tag="VFU72_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423349.1"
                     /translation="MIRDETVVERNDARAAALAGERAVAELLRFPVVSSEAATAEDAL
                     PPRLVQLFLGLMRDQQIDVNSLLEHPGIRYKFIQDVLRDPSMTEADVLRGGQLFGIDF
                     TRTRAVILVDAREYLIGADPAGAGPTPDLVQRRAQKIINSVVSFFALPSETICASIGE
                     GEIAILKASTTQDLAGWVEGRGENRDGGGSWANLSALKRAAEDLLRSIQRDTGAEITI
                     GIGRYHPGVRGLAHSYQDARTALSLGRQSQGGNQVYCLDQLGIASFVGLNDERTKFGL
                     AAHLLSPLDHAPELLETLDVFFRENCCPSTTAYRLEIHRNTLRYRLDKIASLTGLDPR
                     AFDDAVQIRLALFLRQLNRNAA"
BASE COUNT          339 a          726 c          648 g          310 t
ORIGIN      
        1 tcccctccgt caggctcaga atgcagagac cgttggcgtt ggtggtgcag ttcgccagcg
       61 gcgcgccgct ggtgtcgctg acggtgacgg cgacgccggc cgcgggcgtg cagtcggagg
      121 gaatcgctcc cttggccagg ctgagcggtc cgggatcgct cagacaattg aaggcggcca
      181 cgcgaaagtc gatgttggcc gccgccgcct gcccggccag cgccacgaag agcatcagcg
      241 ccgcgaccaa gaccaccacg acgcgtgccc gacgcaccca ctcccccacg acgatccctc
      301 cccgtcccct ggcgaacccc ttgacgccgg ctcactctag ctgagtgctg ccgctgagtc
      361 aacgaggttg cggcgtgccg gttgcgggcg cggccgggag accagctact gtcggcggga
      421 cctggaagcc ggcggcgccg cggccgtcgg agacgtgcgc ttcggcggcg cgggcggttc
      481 ggatacggcc ggcgaacccc cggtcgcggc gaagagggcc agcgaccggc gtcaaccacc
      541 ccccaagccg caccgtgtct tggggtcacg gcggcagcat ggctgaaagc gcgtagtcgt
      601 gcgacgatta ttgtcacacc aacatttatc tggcacagat tatgcattat cgcgctccga
      661 agttcggtca gcgggttgga cgattcctcc catcaccgag cgctttcccc ctagaatgcg
      721 aggttcgggc attgaacgta gtgatacgag acgagacggt tgtcgagcgc aacgacgcca
      781 gagccgcagc gctcgccggt gagcgcgccg tcgcggagct gctgcggttc ccggtggtct
      841 ccagcgaggc ggcgaccgcc gaggatgcgc tgccgccccg gctggtacag ctctttctcg
      901 ggctgatgcg cgatcagcag atcgacgtca acagcctgct cgaacacccc ggcatccggt
      961 acaagttcat ccaggacgtg ctgcgcgatc cctcgatgac cgaggccgac gtgctgcgcg
     1021 gcggccagct cttcggcatc gacttcaccc gcacccgcgc cgtgatcctg gtcgacgccc
     1081 gcgagtacct gatcggcgcc gatccggccg gcgccggccc gacacccgac ctggtgcaac
     1141 ggcgggcgca gaagatcatc aacagcgtgg ttagcttctt cgcgctgccc agcgagacca
     1201 tctgcgcctc catcggcgag ggtgagatcg ccattctcaa ggcgagcacc acgcaggacc
     1261 tggcgggctg ggtcgaaggg cgcggcgaga accgcgacgg cggcgggagc tgggccaacc
     1321 tcagcgcgct caagcgcgcc gccgaggatc tgctgcgctc gatccagcgc gacaccggcg
     1381 ccgagatcac catcggcatc ggccgctacc accccggcgt gcgcggccta gcgcactcct
     1441 atcaggacgc gcgcacggcg ctctcgctgg ggcgccaatc gcagggcggc aaccaggtct
     1501 actgcctcga tcagctcggc atcgcctcct tcgtggggct gaacgacgag cgcaccaagt
     1561 tcggactggc cgcgcacctg ctcagcccgc tcgaccacgc gcccgagctg ctggagaccc
     1621 tcgacgtctt cttccgcgag aactgctgcc cctcgaccac ggcctaccgc ctcgagatcc
     1681 accgcaacac gctgcgctac cgcctcgaca agatcgcctc gctgaccggc ctcgacccgc
     1741 gcgccttcga cgacgcggtc cagatccgcc tggcactctt cctccgccaa ctgaatcgca
     1801 acgccgcctg actgtgcact tgcccaacgg ctcccaggcc tgattgggca aatgcacaat
     1861 gaaacgattg agctactgtt cacctgcccg atgcaggaag tagcgatcgt acgctacttt
     1921 ttgatggacg ccgtcgcggg gtcggggtga gccatccctg agcgctcccc tggcgccgcc
     1981 ccgccagacc gatgggagta tcagcgtgca ggaagacatc ctc
//
LOCUS       DASUBI010000382         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_764496, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000382 DASUBI010000000
VERSION     DASUBI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764496"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..499)
                     /locus_tag="VFU72_07420"
     CDS             complement(74..499)
                     /locus_tag="VFU72_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5423350.1"
                     /translation="MTVHGQSRRTGYDYGMTGSYSYGLHGNYDYGAFLDHYDQPIHQI
                     PVWQFGQPYNPWTAHGPQTGKGPKRSDARLQEDVSDQLHDHAWLDARDVEVRVSQGEV
                     TLTGSVATRQDKRLAEELADTVPGVVDVHNRLTIQTGQG"
     gene            657..1778
                     /locus_tag="VFU72_07425"
     CDS             657..1778
                     /locus_tag="VFU72_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5423351.1"
                     /translation="MRESRLRVGVLGAGNWARSAHIPGWQRDPRAEVVAVADVEPELA
                     GEVARELGIPEASGDWQALVARPDIDVIDVVTPSHTHFELAWAALEAGKHVLCEKPVA
                     YDFRDTLRAAELARRKGLKTKLGFTFRYSPGVQYAKAMIDEGFTGTPYIFNGYEQNSQ
                     WLDPQTPLRQVDHEADQSRLQTSSLEGYGAPVIDISHWWVGADYTRVVGTLKNFVPER
                     VVRATGQMMRMNIDDGDIYLAEYTNGAIGSIQTSYVTVGNYPGIEARLYGSEGAIICR
                     LVEEYGVAETIKVATKDAVEFRELEIPQRFYPAGGHPRESWRSLFYANLIKDFIDEIQ
                     DGGPRNQGNFDDGAWVQEAINAVERSFHERRWVDLPLER"
     gene            1779..3461
                     /locus_tag="VFU72_07430"
     CDS             1779..3461
                     /locus_tag="VFU72_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5423352.1"
                     /translation="MASAAPAFTAWRDDFLAAYFRQNPVNATFIGVHAHDHRLPDYSE
                     HGLGDRRAEIETLLARLETLPAEPLSEAEALDRTLAEGHLRIQHWELDSAQFRHGNPS
                     LFISEAVFGVMSLFLRRFAPLDERVESAVARLHAIPALMAQGKEQLHAAPAAWTARAL
                     DECAGTLAFLGEGIDLLLAGEGIDDARARRAADVATAAVVEFQHHLETETLLRQPEGY
                     AAGGEALDLLLKHGHFLAQDAAVIEAYAADQLAESEAKLREGAATLGAADWQAALARL
                     EDSHPSAAGYLPRFEEIWRAARAHTERHELVSWPDYPLRYVPRPAWTRAAAPHLYFLF
                     YRAPAAFDRVPVVDYLVAPLDGLDAEQQERLLRATNDSVITLNHVVHHGGLGHHVQNW
                     YAYNRAESRLGQVAAVDCASRIAMFCGGTMAEGWACYATELIAEAGFLTPLQQLSVQH
                     SRARMAARAIVDVRLHTGRLTLEEAARFYQERTGMSAGAARAEAVKNSLFPGAALMYL
                     LGTDQIWSLRRELERRAGFSLRAFHDRFLSYGSVPVALIAQAMRAEAGFETQ"
     gene            complement(3455..4345)
                     /locus_tag="VFU72_07435"
     CDS             complement(3455..4345)
                     /locus_tag="VFU72_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5423353.1"
                     /translation="MLRRMGTPCARTRLPGVALALVLLLAGRLLPLGAGATVGSTGVV
                     GPKHYYLALGDSVAFGYQPNLDFSHGYTAQWFSGDLAAKGSQEYVNYACNGETSVTFV
                     NGNCPYSWLLHDHYAGAQLSAAVAFIQAHPGQVSPVTLDIGANDMLDDINDATCTIDL
                     NAWNQDLQALDQNLNVILQALTGALTDAGGNRTGDLILMNYYNPYQNDCTGSSVDSNA
                     YTQQLNALIASHAAAYNLPVADVYTAFGSSAAASNPALCGDTWMCRRVHDIHPSGGRW
                     GEPGNGYGVIAAAFKQTIGY"
BASE COUNT          707 a         1591 c         1631 g          726 t
ORIGIN      
        1 aggctagcgc cgcgggcttt agcccggcgc gtccccggcg ccgagcctcc atggacgtgg
       61 tacccgctac ccgctacccc tgtcccgtct gaatcgtcag ccggttgtgg acgtcgacca
      121 cgccggggac ggtgtcggcc agctcctcgg ccaggcgctt gtcctggcgc gtggcgaccg
      181 agccggtcag cgtcacctcg ccctgactga cccgcacctc gacatcgcgg gcatcgagcc
      241 aggcgtggtc gtggagctgg tcgctgacgt cctcttgcag gcgcgcgtcg gagcgcttcg
      301 ggcccttgcc tgtctggggg ccgtgtgcgg tccaggggtt gtacggctgg ccgaactgcc
      361 agaccgggat ctgatggatc ggctggtcgt agtggtccag gaaggcgccg tagtcgtagt
      421 tgccatgcag gccgtaggaa tacgagccgg tcatgccgta atcgtagccg gtgcgccggc
      481 tctgcccgtg gacggtcatc gcgccctcct ctgactctac tccgcgccgg gcgcccgcgc
      541 cccggtcgat cactggccga ttcgcatggc tcgtgccggg tcggctgttg ccggaaagcg
      601 ccgcaggctc tatacccatg cagcagcgaa cggcgaatag cggagggaga gtcgaaatgc
      661 gcgagagcag gctgcgggta ggcgtgttgg gagccggcaa ttgggcgcgc tcggcgcaca
      721 taccgggctg gcagcgcgac ccgcgcgccg aggtcgtggc cgtcgccgac gtcgagccgg
      781 agctggccgg cgaggtggcg cgcgagctcg gcatccccga ggcgagcggc gactggcagg
      841 cgctggtggc gcggccggat atcgacgtga tcgacgtcgt caccccctcg cacacgcact
      901 tcgaactggc ttgggccgcg ctggaggcgg gcaagcacgt cctctgcgag aagccggtgg
      961 cctacgattt ccgcgacacc ctgcgcgccg ccgagctggc acgccgcaag gggctcaaga
     1021 ccaagctcgg cttcaccttc cgctacagcc cgggcgtgca gtacgccaaa gccatgatcg
     1081 acgagggctt caccggcacg ccctatatct tcaacggcta cgagcagaac tcgcagtggc
     1141 tcgatccaca gaccccgctg cgccaggtgg accacgaagc cgaccagtcg cgcctgcaga
     1201 cctcctcact ggagggctac ggcgcgccgg tgatcgacat cagccactgg tgggtcggcg
     1261 ccgactacac ccgcgtggtc ggcacgctca agaacttcgt gcccgagcgc gtggtgcgcg
     1321 ccaccggcca gatgatgcgc atgaatatcg acgacggcga catctacctg gccgagtaca
     1381 ccaacggcgc catcggctcg atccagacca gctacgtgac cgtcggcaac taccccggca
     1441 tcgaggcgcg cctgtatggc agcgagggcg cgatcatctg ccggctggtc gaggagtacg
     1501 gcgtggccga gacgatcaag gtagcgacca aggacgccgt ggagttccgc gagttggaga
     1561 tcccgcagcg cttctacccg gccggcggcc acccgcgcga gtcctggcgc tcgctcttct
     1621 acgccaacct gatcaaggac ttcatcgacg agatccagga cggcggcccg cgcaaccagg
     1681 gcaacttcga cgacggcgcc tgggtgcagg aggcgatcaa cgcggtcgag cgctccttcc
     1741 acgagcggcg ctgggtcgac ctgccgctgg agcgctagat ggcgagcgcg gcccccgcct
     1801 tcacggcctg gcgcgacgac ttcttggcgg cgtactttcg ccaaaacccg gtcaacgcga
     1861 ccttcatcgg cgtgcacgcc cacgaccacc ggctgccgga ctattcggag cacgggctgg
     1921 gcgaccggcg ggccgagatc gagacgctgc tggcccggct ggagacgctg ccggccgagc
     1981 cgctgagcga ggccgaagcg ctcgatcgca cgctggccga gggccacctg cgcatccagc
     2041 actgggagct cgactcggcg cagttccgcc acggcaaccc ctcgctcttt atctccgagg
     2101 cagtattcgg cgtcatgagc ctcttcctgc gccggttcgc gccgctggat gagcgagtcg
     2161 agtccgccgt ggcccgcctg cacgcgatcc cggcgctcat ggcgcagggc aaggagcagc
     2221 tgcacgcggc gccggccgcc tggaccgcgc gcgccctcga cgagtgcgcc ggcacgctgg
     2281 ccttcctggg cgagggcatc gacctgttgc tggccgggga ggggatcgac gacgcgcgtg
     2341 cgcgccgggc ggcggatgtg gccaccgccg cggtcgtgga gttccagcac catctcgaaa
     2401 ccgagacgct cctgcgccag ccggagggct acgcggccgg cggcgaggcg ctcgatctcc
     2461 tgttgaagca cgggcacttc ctggcgcagg acgcggcggt gatcgaggcg tacgccgccg
     2521 atcaactggc cgagagcgag gcgaagctcc gggagggcgc cgcgacgctc ggcgccgctg
     2581 actggcaggc ggcgctggcc cggttagagg acagccaccc cagcgccgcc ggctacctac
     2641 cgcgcttcga ggagatctgg cgggcggcgc gtgcgcacac ggagcggcac gagctggtga
     2701 gctggccgga ctatccgctg cgctacgtcc cgcgcccggc ctggacccgc gccgccgcgc
     2761 cgcatctcta cttcctcttc taccgcgcgc cggccgcctt cgatcgcgtc ccggtggtgg
     2821 actacttggt cgcgccactc gacgggcttg atgcagagca gcaggagcgc ttgctgcgtg
     2881 ccaccaacga cagcgtgatc acgctcaacc acgtcgtcca ccacggcggg ctggggcatc
     2941 acgtgcagaa ctggtacgcc tacaaccgtg ctgagtctcg ccttgggcag gtggcggcag
     3001 tcgattgcgc ctcccgcatc gccatgttct gcggcggcac catggccgag ggctgggcct
     3061 gctatgccac cgagctgata gccgaggccg gcttcctgac gccgctccag cagctcagcg
     3121 tgcagcacag ccgggcgcgc atggcggcgc gggccatcgt cgacgtgcga ctgcacaccg
     3181 gccgcctgac gcttgaggag gcggcacgct tctaccagga gcgcacgggg atgagcgcgg
     3241 gcgcggcgcg cgccgaggcg gtcaagaact cgctgttccc cggcgcggcg ctgatgtacc
     3301 tactcggcac cgaccagatc tggagccttc gccgcgagct ggagcggcgc gcgggcttca
     3361 gcctgcgcgc cttccacgac cgctttctct cctacggctc ggtgccggtg gcgctcatcg
     3421 cccaggcgat gcgggccgaa gccggattcg aaactcagta gccgatcgtc tgcttgaagg
     3481 ccgcggcgat cacgccgtag ccgttgcccg gttcgcccca gcgcccgccc gaggggtgga
     3541 tgtcgtgcac ccggcggcac atccaggtgt cgccgcagag cgccgggtta ctggccgccg
     3601 cactggagcc gaaggcggta tagacgtcgg ccaccggcag gttgtacgcg gcggcgtggc
     3661 tcgcgatcag cgcgttcaac tgctgcgtgt aggcgttgct gtcgacgctc gagccggtgc
     3721 agtcgttctg gtaggggttg tagtagttca tcaggatcag atccccggtg cggttgccgc
     3781 cggcgtcggt gagcgcgccg gtcagcgcct gcaggatcac gttgagattc tgatccagcg
     3841 cctgcaggtc ctggttccag gcattgagat cgatggtgca ggtggcgtcg ttgatgtcgt
     3901 cgagcatgtc gttggcgccg atatcgagcg tcaccgggct gacctggccg gggtgtgcct
     3961 ggatgaacgc gaccgccgcg ctcaattgcg cgccggcgta gtggtcgtgc agcagccaac
     4021 tgtaggggca gttgccgttg acgaaggtga cgctggtctc gccgttgcag gcgtagttga
     4081 cgtactcctg gctgcccttg gcggccaggt cgccggagaa ccactgcgcc gtatagccgt
     4141 gtgagaagtc gaggttcggc tggtagccga aggccaccga gtcgcccagc gccaggtaat
     4201 agtgcttcgg accaacgacg ccggtcgatc cgacggtcgc cccggctccc agcggcagga
     4261 gtctccccgc cagcagcagc acaagcgcca acgccacgcc gggcagccgc gtgcgcgcgc
     4321 acggcgtccc catccgcctc agcatcgcca atcccttcca cgcggggcag cgagcgggtc
     4381 gtccccggcc ccggcgagct gtcttgtccc tctagcggca tcgtagaagc gggccgagat
     4441 gatgtcaagc agatccggct cgcggcccgg cttctgcggg ccagttcatt accttcaggc
     4501 gaaggggtta tcggagacga ataacgagag acggagtgaa ttatgagtct gagcgcggcc
     4561 aaccggctgg tcgagccggt cagtcaacag gatcacgtgc tcggtccggc ggatgccccg
     4621 gtgacgctgg tcgagtacgg cgacttcgag tgccc
//
LOCUS       DASUBI010000383         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1281523, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000383 DASUBI010000000
VERSION     DASUBI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281523"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2398)
                     /locus_tag="VFU72_07440"
     CDS             complement(<1..>2398)
                     /locus_tag="VFU72_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5423354.1"
                     /translation="EATTAPAAGRARPLLVGREREQGLLRGQLDRMLGGHGGVVLVGG
                     EAGIGKTTLVEDLSVQAEEQGALVLWGHAYDLSVTPPYGPWLEILRQYRSLAAADLPP
                     VPPFVFDTAELAKVGSQETLFTTMTGFFTTLAARRPLLLVLDDLQWDDQASLDFLRHL
                     ARQLAGQRVLLLATYRSDEVTRRHPLAALLPLLVREAGAERVDVQPIQQAGRRALIRS
                     RYALGEADQTRLERYLEEHAEGNPLYAGELLRTLEEEGVLTQQAGDWTLGNLEQVRLP
                     ALLRQVIEGRLARLDEECQRLLGVAAVVGQVVPLAVWAAAGEVDEDTLLDHAERAEEA
                     RLLEGLPAGDGVRFSHALIREALYDGIPSLRRRRLHQRIGEVLSAAPHPDPDIVAYHL
                     QRAGDPRAIDWLLQAAERAWSVYAPATAIAHFDQAEALAEPQGRALPASAYLIRGQAQ
                     RLVGNFAPARDDLEQALTLARQAGDGRTEWQALTELGMLWTARDYAKTGEYFRQALAL
                     ARTLDAPDLTAHALNRVGNWHVNVEELLEGVQNHREALAIFERLDDRAGLAGTYDLLA
                     LGCFLSGDLTQGGDYLRHAVALFRASGDRAGLASCLSLVTMQGVNNLTETVFIEQETV
                     AARLSASEEAFAIAHAMGWRVGEAYALWTRAFCLGASGAYGAALDAVQRSLEIAEEID
                     HQQWLSAAHAAAGAIYFDLLALPQAQHHYEQSFSFARQNASTTWMYAASGLVASVLLA
                     GGDRAGAEAALDAILDPDTPMYSLPQRRLWVTRVELVLAQGDPEAALGLLDRMAAANP
                     "
BASE COUNT          363 a          864 c          833 g          338 t
ORIGIN      
        1 ctggggttgg ccgcggccat ccggtcgagc agcccgagcg ccgcctcggg atcgccctga
       61 gccagcacga gctccacccg cgtgacccac agacgccgtt gaggcagcga atacattggc
      121 gtatcggggt cgaggatggc atcgagggcg gcctcggctc ccgcgcggtc gcctccggcc
      181 agcagcacgc tcgcgacgag gccggatgcc gcatacatcc aggtcgtgga ggcgttctgg
      241 cgcgcgaagg agaacgattg ctcgtaatgg tgctgtgcct gaggaagcgc cagcaagtcg
      301 aagtagatcg ctccggcggc ggcgtgcgcg gccgacagcc actgttggtg gtcgatctcc
      361 tcggcgatct caaggctccg ctggaccgca tcgagcgccg cgccatacgc tccgctcgcc
      421 ccgagacaga acgcgcgcgt ccacaacgcg tacgcttccc ccacgcgcca gcccatcgcg
      481 tgggcgatcg cgaacgcctc ttcgctggcg ctcaggcgcg cggccacggt ctcctgctcg
      541 atgaagaccg tctcggtcag attgttcacg ccttgcatcg tcaccagaga caggcacgag
      601 gcgagcccgg cacgatcgcc ggaggcgcga aagagcgcga ccgcatgtcg gaggtagtcg
      661 cctccctggg tgaggtcccc gctgagaaag cagcccaacg ccaagagatc ataggtcccc
      721 gccaggccgg cccgatcgtc taatcgttcg aagatggcca gcgcctcgcg atgattctgg
      781 acgccttcca gcagctcctc gacattcaca tgccagttgc cgacgcgatt gagcgcgtgc
      841 gcggtgagat ctggggcatc cagcgtccgg gccagcgcca gcgcctgccg aaaatactcg
      901 cccgtcttgg cgtagtcgcg ggccgtccag agcatgccga gctcggtgag cgcctgccac
      961 tcggtccgtc cgtcgccggc ttgccgggcc agcgtcagcg cttgttcaag atcgtcgcgc
     1021 gccggcgcga agttgccgac gaggcgctgc gcctgcccgc gaatcaggta ggcggaagcg
     1081 gggagcgcgc gcccctgcgg ctccgccagc gcctcggcct gatcgaaatg cgcgatggcg
     1141 gtggcgggcg cgtagacgct ccaggcccgc tcggcggcct ggagcagcca gtcgatcgcg
     1201 cgcgggtcgc ccgcccgctg gagatggtag gcgacgatgt cggggtccgg atggggggcg
     1261 gcgctgagca cctcgccgat ccgctgatgc agccgccgcc ggcgcagcga ggggatgccg
     1321 tcgtagagcg cctcccggat cagggcgtgg ctgaagcgca cgccgtcgcc cgcgggcagc
     1381 ccttcgagca gcctggcctc ctcggcgcgc tcggcgtgat cgagcagggt gtcctcgtcg
     1441 acctcgcccg ccgccgccca gaccgccagc ggcactacct gaccgacgac cgccgccacc
     1501 cccagcagcc gttggcactc ctcgtccagg cgcgccagcc ggccctcgat gacctgccgc
     1561 agcagcgccg gcaagcgcac ctgctccagg ttgccaagcg tccagtcgcc agcctgctgg
     1621 gtcagcacgc cctcctcttc cagggtgcgc agcagctcgc cggcatagag cgggttgccc
     1681 tcggcgtgct cctccaggta acgctccagc cgggtctggt cggcctcccc cagcgcgtag
     1741 cggcttcgga tcaacgcccg gcggcccgcc tgctggatcg gctgtacgtc cacccgctcg
     1801 gcgcccgctt cacgaacgag cagcggcagc agcgcggcga ggggatggcg gcgcgtcacc
     1861 tcatccgagc gataggtggc cagcagcagg acgcgctggc cggcaagctg gcgagccaga
     1921 tggcgcaaga agtcgagcga ggcctggtcg tcccactgga ggtcgtcgag caccagcagc
     1981 agcggacgcc gcgccgccag ggtggtgaag aagcccgtca tcgtcgtgaa gagcgtctcc
     2041 tgggaaccga ccttcgccag ctccgcggtg tcgaagacga aaggcggcac gggcggcagg
     2101 tcggcagcgg ccagcgaacg gtattggcgc aggatctcca gccaggggcc gtagggcggg
     2161 gtgacactca ggtcgtaggc atgcccccag aggacgaggg ctccttgttc ctcggcctgc
     2221 acgctcagat cctcgaccag tgtcgtcttg ccgatccccg cttcgccgcc gaccagcacc
     2281 acaccgccgt gcccgccgag catgcggtcg agctgccccc gcagcaggcc ctgttcgcgc
     2341 tcgcggccga cgagcaaggg gcgcgcccgg ccggcggcgg gtgccgtcgt tgcttccg
//
LOCUS       DASUBI010000384         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_820193, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000384 DASUBI010000000
VERSION     DASUBI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820193"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..>2322)
                     /locus_tag="VFU72_07445"
     CDS             complement(75..>2322)
                     /locus_tag="VFU72_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5423355.1"
                     /translation="RPRRGEQAMSATAATKIHPQHQRRQALVYVRQSTTKQLAVNQES
                     TRRQYQLAERAQALGWPPPRVVVIDDDLGLSGSSSEARAGFQRLVSAVGLGEVGAIFV
                     TEISRLSRRNSDWHRVIELCAVFDTLIADEDGIYDPADPNDRLVLGLKGTLFAAELHI
                     LRARMRGGLLNKARRGALALRLPVGYRRLPDGTVVLEPDEAVRGVLATVFSQFPQLGS
                     ARAVQRYLQAHGLQMPRYHQTGPDAGRIVWVAPTYQMVQMVLHSPVYAGLFVYGRRRE
                     VITPGDPPQVTTHRVPVDEWAIVVPGVYPAYVTEDQYRANRRRLCDNQYNFVKRRRGA
                     VREGPGLLVGLLVCGRCGGRLTPTYSAAHHAYVCRRAKATYGTPTCQSFPQRELDQTV
                     AAAFLAAVQPAALETLLTALAAHEAERQAVARHWAQRLERARYEAARAQRQYDACEPE
                     NRLVARTLEARWEEALAAMAQVEQEYAAAERSALAPLTAAEQAAARALASDLPAVWQA
                     ATTTAADRKRLLRLVIQDVTVTVAPGGRDAAVTILWSGGATTAHTIIRPPRGWHCTTD
                     AAVVARLRALAQHRPDHQIADLLNAEGLTTQTGKPWTHQRVASIRKQHAILTACPVGT
                     AIHAVRGDGFMPVALAAERLGVSRSLVHVWVGLGALVGEQRVAASKRWVRLTAADAAR
                     LDGKHDWGHLPTLRQVMRERGWDRTETWARVRAGEYVAHRHPTRQRWEWRLQQARPSR
                     PPGPSDVR"
BASE COUNT          298 a          839 c          844 g          341 t
ORIGIN      
        1 ggccgccgag ttgctcttcc agttcttcgc ccagtgatat tcatactgcg tggtgccttt
       61 ctcatgacat ccaactaacg cacgtcgctt ggacccggtg gtctgcttgg ccgggcctgc
      121 tggaggcgcc attcccaccg ctgccgcgtc gggtgacggt gggcgacata ctcgccggcg
      181 cggacacgcg cccaggtctc cgtccggtcc cagccccgct cgcgcatcac ctggcggagc
      241 gtggggaggt ggccccaatc gtgcttgcca tccagccggg cggcatccgc cgccgtcagg
      301 cgcacccagc gcttggaggc ggccacgcgc tgctcgccaa cgagcgcccc gaggcctacc
      361 cagacatgca ccagcgagcg cgagacgccc agccgctcgg cggcgagggc gacgggcata
      421 aacccgtcac cgcgcacggc gtggatggcc gtgccaaccg ggcaggctgt aaggatggcg
      481 tgctgtttgc ggatcgacgc gacccgctga tgggtccacg gcttgcccgt ctgcgtcgtc
      541 aggccctcgg cattgagcag atcggcgatc tggtggtcgg gccggtgctg cgccagcgcg
      601 cgcaggcggg cgacgacggc cgcgtcggtc gtgcagtgcc agccccgcgg cgggcggatg
      661 atggtgtgcg cggtggttgc cccgccgctc cagaggatgg tgacagcggc gtcgcggccg
      721 ccgggcgcga cggtgacggt gacgtcctgg atgaccagcc gcagcaggcg cttgcgatcc
      781 gccgccgtgg tcgtcgcggc ctgccagacc gccggcagat cgctcgccag cgcgcgggcc
      841 gccgcctgct ccgccgccgt cagcggtgcg agggcgctac gctcggctgc ggcatactcc
      901 tgctccacct gcgccatggc cgccagcgcc tcctcccagc gggcctccaa ggtccgcgcg
      961 accaggcggt tctccggctc gcaggcgtcg tactgccgct gcgcccgcgc cgcctcgtaa
     1021 cgcgcccgct ccagccgctg cgcccagtgg cgggcgacag cctgccgttc cgcctcgtgc
     1081 gcggccagcg cggtcagcag cgtctccagc gccgcgggct gcaccgccgc caggaaggcc
     1141 gcagcgaccg tctggtcgag ctcgcgctgg gggaaggatt ggcaggtcgg ggtgccgtag
     1201 gtcgccttcg cgcggcggca gacataggcg tggtgggccg cgctgtaggt cggcgtcagc
     1261 cgccccccgc agcggccgca gacgagcagc ccgaccaaga gccccggtcc ctcgcgcacc
     1321 gccccccgcc tccgtttgac gaagttgtac tgattgtcgc acagccgccg gcggttggcg
     1381 cggtactggt cctcggtgac gtaggccggg tagacgcctg ggacgacgat ggcccactcg
     1441 tcgaccggca cccgatgagt ggtgacctgc ggcgggtccc ccggcgtgat gacctcgcgg
     1501 cgtcggccat agacgaacag gccggcgtag accgggctgt gcagcaccat ctgcaccatc
     1561 tggtaggtcg gcgcgaccca gacgatgcgc cccgcgtcgg ggccagtctg gtgataccgg
     1621 ggcatctgca ggccgtgggc ctgcaggtag cgctgcaccg cgcgggcgct gcccagctgc
     1681 gggaactggc tgaagaccgt cgccaggacg ccgcgcaccg cctcgtccgg ctcgaggacg
     1741 accgtgccgt cgggcaggcg gcggtagccg accggcaggc gcagcgccag tgcgccccgc
     1801 cgggccttgt tcaagaggcc gccgcgcatg cgggcgcgca ggatgtgcag ttcggccgca
     1861 aagagcgtcc ccttcagccc gaggaccagc cggtcattag ggtcggccgg gtcgtagatg
     1921 ccgtcctcat cggcgatgag ggtgtcgaag acggcgcaca gctcgatgac gcggtgccag
     1981 tcgctgttgc ggcgcgacag gcgcgagatc tcggtcacga agattgcccc cacttccccg
     2041 aggccgaccg ccgagaccag gcgctggaag ccggcgcgcg cctcactact actgccggag
     2101 aggccgaggt cgtcgtcgat gaccacgacg cgcggcggcg gccagccgag cgcctgcgcc
     2161 cgctcggcca gctggtactg ccgccgggtg ctctcctggt tgacggcgag ttgcttggtc
     2221 gtcgactgcc gcacgtagac cagcgcctgg cggcgctggt gctgcgggtg gatcttggtt
     2281 gccgcggtcg cactcatcgc ttgctctccc ctccggggtc gg
//
LOCUS       DASUBI010000385         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_682431, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000385 DASUBI010000000
VERSION     DASUBI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682431"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFU72_07450"
     CDS             <1..854
                     /locus_tag="VFU72_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5423356.1"
                     /translation="LTLWRTRPEQPYAPEDQDFLLALASRAALAVENARLIQQLQQAV
                     QAREEFLSIAAHELKTPMTTVKGFAALLVDQLRGGEPDPEIVQECGDELRAQVDRFET
                     LVNDLLDASRAQRQRLDLHVEPLDLTELAVRVVARFQRGAGADGHRVALSAGEPVEGV
                     WDPDRLDQVLTNLLSNAVKYSPEGSEIRVAVWRHGDQAALSVSDHGIGMSPDEQARLF
                     TPFVRTSAARQTAPGTGLGLYISRQILEQHGGSIEVESAVGAGTTMVVRLPIRREPAT
                     DSDAPAR"
     gene            complement(887..1213)
                     /locus_tag="VFU72_07455"
     CDS             complement(887..1213)
                     /locus_tag="VFU72_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423357.1"
                     /translation="MSGTAEIEMQLLQALVDTEQSQAHLPGDSFVVVPLGYQGMAVFY
                     PHARPITGYERAHLDHLQELGLLRITRQRSGSDLCQITQEGYRTAERQPGWPAPLNEH
                     TLKLSG"
     gene            1881..2738
                     /locus_tag="VFU72_07460"
     CDS             1881..2738
                     /locus_tag="VFU72_07460"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5423358.1"
                     /translation="MTTAGNQQRQVLVGTDWVAEHLNDPNVRLVEVDVDTSAYDTGHA
                     PGAVGWNWKTDLQRHPVRDIPTEQEWEALLGRSGIGNDTTVILYGDNNNWFAAFAYWL
                     FRMYGHQDVRLMNGGRAKWLAENRPQTTEVPQPAPRQYQAGEFNADLRAFRDNVQRAI
                     NEPGSAMVDVRSPAEFSGALLAPENLPQEGAQRGGHVPSAANVPWSTAVAPDGTFKPL
                     AELRQIYDGKGVTPDKDVIAYCRIGERSAHTWYVLHELLGYPNVHNYDGSWTEWGSLI
                     GAPIATGEE"
BASE COUNT          441 a          991 c         1005 g          429 t
ORIGIN      
        1 cgctgacgct ctggcgcacg cgcccggagc agccctacgc gcccgaggac caggatttcc
       61 tgctggcgct ggcctcacgc gccgcgctgg ccgtcgagaa cgcgcgcctg atccagcagt
      121 tgcagcaggc ggtccaggcg cgtgaggagt ttctctcgat cgcggcgcac gagctgaaga
      181 cgccgatgac cacggtcaaa gggttcgccg cgctcctggt ggatcagttg cgcggcggcg
      241 agcccgatcc cgagatcgtg caggagtgcg gcgacgaact gcgcgcgcag gtcgaccgct
      301 tcgaaacgct ggtcaacgac ctgctggacg cctcccgcgc gcagcgccag cgactcgacc
      361 tccacgtcga gccgctggat ctgaccgagc tcgccgtccg cgtggtcgct cgcttccaga
      421 gaggggcggg cgccgatggg caccgggtgg cgctcagcgc gggggagccg gtcgaggggg
      481 tctgggatcc ggatcgtctc gatcaggtgc tgaccaatct cctctccaac gcggtcaagt
      541 attcgcccga ggggagcgag atacgggtcg cggtttggag gcatggtgac caggcggcgc
      601 tctcggtcag cgatcacggc atcggcatga gccccgacga gcaggctcgc ctcttcacgc
      661 ccttcgtgcg cacgtcggcg gcgcgccaga cagcgcccgg caccgggctc gggctgtaca
      721 tctcgcgcca gatcctggag cagcacggcg gctcgatcga agtcgagagc gccgtgggcg
      781 ccggcacgac gatggtcgtg cgcttgccga tccggcgcga gcctgcgacg gactccgacg
      841 cgcctgcgcg ttgacccaat cgcgagcaca ggccgccgaa ggggcgctag cccgagagct
      901 tgagggtgtg ctcgttgagc ggtgccggcc agccgggctg tcgttcggcg gtccggtagc
      961 cttcctgcgt gatctggcag aggtcggagc cactccgctg gcgcgtgatg cgcagcaggc
     1021 cgagctcttg caagtggtcg agatgggcac gctcgtagcc ggtgatcggc cgggcgtggg
     1081 ggtagaagac cgccatcccc tggtagccga gcggcaccac gacgaaggag tcgccgggca
     1141 ggtgcgcctg tgactgctcg gtatcgacca atgcctgaag gagctgcatt tcaatctctg
     1201 ccgtgcccga caccggccac ctcccgaacg ctcgtcgccg tatttgctcc gagcataccg
     1261 actgatcgtc gcacccacgt ggagaagcgt gcgcctgtgc gacaatcgcg tgacaaagcc
     1321 gcgctccagc ggcccagcgg cgcgtcttcc ggcggcgcga cggccgccga gacgtgagcg
     1381 tttacggtcg tgcgcgtgca tcgctgaagc gcgggcattt tcaggggctc aactacggca
     1441 cacccgcgcc ctgtggccgc ccatcccggc tgcgtccagc gcgcgttgcg ccagacgctc
     1501 ccggcgcggc ccgcatgagt tcggatcgac ccacagcgcc ttctcaccgc ccaagacggg
     1561 ccggctggcg tcaggcgcct gctgcgatgt cgcggagtat ggcctggatc atgtgagaac
     1621 cccgtgacaa ccatgcgcgc ggtgttgcaa aaacgtgaca atcggcccgt cgcgagtcgg
     1681 cgatggccac gagattggta tgcgcgtggg ggtggccgcc gcgcctgcgt ggcgctgccc
     1741 ctgcctcctt gaacggcagc cacccaatct tccctgggga atgccgcctg cctgcctcgc
     1801 gttgtcaccg acgagctccc cggtcaggcg tgggccgcgc cggggctgtg gcgagcgcgt
     1861 ttccatggaa ggaccggggc atgacgacag caggaaatca gcagcgccag gtgctggtgg
     1921 ggaccgattg ggtggccgag catctcaacg acccgaacgt gcggctggtc gaggtcgacg
     1981 tcgacacctc cgcctacgat accgggcatg cccccggcgc ggtcggctgg aactggaaga
     2041 ccgacctgca gcgccacccg gtgcgcgaca tcccgaccga gcaggagtgg gaggcgttgc
     2101 tgggccgctc cggcatcggc aacgacacca ccgtcatcct ctacggcgac aacaataact
     2161 ggttcgccgc cttcgcctac tggctcttcc gcatgtacgg gcatcaggat gtgcgcctga
     2221 tgaacggcgg gcgcgccaag tggctggccg agaaccggcc gcagacgacc gaggtccccc
     2281 agcccgcgcc gcggcagtat caggccggcg agttcaatgc cgatctgcgc gccttccgcg
     2341 acaatgtgca gcgggcgatc aacgagcccg gctcggccat ggtcgacgtg cgtagcccgg
     2401 cggagttcag cggggcgctg ctggcgccgg agaacctgcc gcaggagggg gcgcagcgcg
     2461 gggggcacgt ccccagcgcg gccaacgtcc cctggtcgac ggcggtggct ccggatggca
     2521 ccttcaagcc gctggccgag ctgcgtcaga tctacgacgg caaaggcgtc acgccggaca
     2581 aggacgtgat cgcctactgc cgcatcggcg agcgctcggc gcacacctgg tatgtgctgc
     2641 acgagctgct cggctacccc aacgtccaca actacgacgg ctcctggacc gagtggggca
     2701 gcctgatcgg cgccccgatc gcgaccggcg aggagtagcc gtccgctcac gcatcccctg
     2761 gcccgcgctc gcgtgcgagc gcgggccgtt gctctgccgg cccctcggca cctcgcggcg
     2821 cgacaggctc tcgccgccca ctcccaggac gccgcagtgt ttctgg
//
LOCUS       DASUBI010000386         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_968391, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000386 DASUBI010000000
VERSION     DASUBI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968391"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFU72_07465"
     CDS             complement(<1..839)
                     /locus_tag="VFU72_07465"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5423359.1"
                     /translation="MRTLTLLPDAQVLHLDALTTTADTIIVVVSTTGDWACCPECCQP
                     SRHRHSRRWRLVADLPWQGLAVRLELHFRRFYCDTPTCPRRIFTERLPTVVAPYGRRT
                     RRLAEVVAAVVFALGGEAGARLLITLGLTASPDTLLRVIQASPAPPVPTPRVLGVDDW
                     ARRRGRSYGTLLVDLERHRPVDVLPDRRAATFAAWLKAHPGSEIICRDRGGAYADGAR
                     QGAPAAIQVADRWHLYANLGELLERLLVRHHALLRRVASAVGAPAATPPPAAEMAPTT
                     PAAT"
     gene            2093..3658
                     /gene="istA"
                     /locus_tag="VFU72_07470"
     CDS             2093..3658
                     /gene="istA"
                     /locus_tag="VFU72_07470"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEU5423360.1"
                     /translation="MAGRSIDLMDVREILRYLQTTTNLSAIQRATGLNRRTLMRYRAW
                     AMEHGLLTRPLPSLEELQQLVARTLTPPAPPQTVSTVEPYRDLVRDLHARGVEGTAIW
                     QRLAEQGYTGSLSSVYRFLHQLSPPVLGVTVRVERAPGSEAQVDFGYAGYLLDDTAGR
                     RRKAWAFVMTLAYSRHQYVEFAWDQTLPTWIALHRHAFTFFGGVPQRVVLDNLKAGVA
                     RACFDDPQIQATYRECAEHYGFLVAPCAPRTPEHKGKVEQGGVHYVKRNFLGGRRPTT
                     LARANADVRAWCETTAGRRRHGTTKEAPLERFAAVERDQLLPLPPESYDLAIWKEVTL
                     HRDCHVVFEQAFYSAPFRLVGQRLRVRGGSREVRLYTTDYQLVATHPRAPGPGTRQTH
                     PDHLPAAKLPQLLWARELCRVLAVEVGPAATAVVQQLLADPVVDRHSRVVRILRLRET
                     VGDARLEAACARALRYDDLTYATIKRILAQGLEAAERDPTPTVAPARTFVRTAADLLG
                     HLFGGVAWTSSTN"
     gene            3637..4431
                     /gene="istB"
                     /locus_tag="VFU72_07475"
     CDS             3637..4431
                     /gene="istB"
                     /locus_tag="VFU72_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEU5423361.1"
                     /translation="MDLQHQLSPKLKQLRLSGVLATLEARHRQALDGQWSYVEFLERL
                     LEDEVERRAQKQLALRIRRGSLNTTKTLEGYDFSFNPAASRPQVLHLAAGDYIRRRRN
                     LLICGPTGVGKSHLAQALAHEAARQGHEVLFVTTDKMLHHLHGGRADGTWERRLAQYL
                     RPDLLVLDDFGLKPLTDPAPSDLYDVINERYEVGSILVTSNRAPAEWPDLFGDPLLAS
                     AGLDRLAHQAETLIITGRSYRAQGRAGVGEERGLLKEEGGGPTPEH"
     gene            complement(4560..4898)
                     /locus_tag="VFU72_07480"
     CDS             complement(4560..4898)
                     /locus_tag="VFU72_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423362.1"
                     /translation="MLCSPHTRPLILLVEDDPAVRDVTVLALQEAGYRVVAVATLAEM
                     ADAPTTTAAWTSPAWLRQAAGAMQVVIFTAYAPSRFAGYAARGFAGLLAQPFDLDAFL
                     TQVQALLQVA"
     gene            complement(5002..5343)
                     /locus_tag="VFU72_07485"
     CDS             complement(5002..5343)
                     /locus_tag="VFU72_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423363.1"
                     /translation="MAPTILVADDVPSIADLLADEGYTVLTAYDGAQALALAAQAYPA
                     LIISDIMMPRLSGVDLVAQLRARAADAGPPVLLLSAGPPPRLPVQTAFLAKPFDLDEL
                     VATVDRLLVSA"
BASE COUNT          973 a         2006 c         2137 g          850 t
ORIGIN      
        1 gtggccgccg gcgtggtcgg ggccatctca gccgctgggg gtggggtcgc tgcgggcgcg
       61 ccgacggcgg acgcgacccg gcggaggagc gcgtggtggc gcaccaggag ccgttccagc
      121 agctcgccga gattggcgta caagtgccag cggtcggcga cctggatggc cgcgggggcc
      181 ccttgtcgcg ccccgtccgc ataggccccg ccccggtcgc gacagatgat ctcgctgccc
      241 ggatgagcct tgagccaagc cgcgaacgtc gccgcacggc gatcggggag gacgtccacc
      301 ggccgatggc gctccagatc gaccaacagg gtgccatagc tgcggccccg gcggcgcgcc
      361 cagtcgtcga cgcccaagac tcgcggggtc gggaccggcg gggcgggact ggcctggatg
      421 acgcgcagca gcgtatcggg actggccgtg agccccaagg taatgagcag gcgggccccc
      481 gcttcgcccc ccagcgcgaa gacgacggcc gcgacgacct cggccaagcg gcgcgtccgc
      541 cgcccatagg gggccacgac ggtcgggagg cgctctgtga agatgcgccg cgggcaggtc
      601 ggcgtgtcgc agtagaagcg gcgaaagtgg agttcgaggc gcaccgccag cccttgccag
      661 ggcaagtcag cgaccaggcg ccagcgccga ctatgccgat ggcgcgacgg ttggcagcac
      721 tcggggcaac aggcccagtc accggtggtg ctgacgacca cgatgatggt gtctgcggtc
      781 gtcgtcaacg cgtcgaggtg cagcacctgc gcgtctggca ggagggtcag cgtccgcatc
      841 ggtagctccc accgtggggt cgcccgcgcc catagatggc ccggcccagt gagtatacgc
      901 cgcccgcgct ggccgctggc gcaccaactt cacgggagag ccacttctcc gtcggaattt
      961 acagacgctg cggcggtgcg ctgctgcgcg gccccagcgt gctcctcgtc ctgctggtcg
     1021 ccctggtgga tcgcctgccc cgccgccccc ggcgcagtgc agctgcggcc ggccggtgac
     1081 ccaccccggc ataagtttcc gctttccgcc gccgttgccc cgaccacaac ggccggtgag
     1141 ggccgctggt gacgggtggc gctgcgtacg ccatgagggc accgtgatca cgatattcga
     1201 ccgcccggcc atctccttgc taaggaggag gtcgctcgtc attaccggtt cttctcagcg
     1261 tggaaatgta gccaatctgt agccgagcgc ctgggacgtc gtggaacgcc gtggccgtag
     1321 cggccggtat tgcggcgtac gaacgaggat cgcggaagca gggcaagagc ggtggacagg
     1381 agaatcaggg acttttaatc agtgggtcaa ttgctgccag aaagcgagga ccgctcgccc
     1441 tattatcgca cgcaccggac cagggccgat agccagggaa tgctggcacc agcgaagcgc
     1501 ggtgcgggga tgtagaccgg catgtagaca acaatgtaga cgagccgctg gcattgcctg
     1561 gaagcggatg gagcattcaa gcacgttttt tgtggtctag cagggaaaat gcattcctat
     1621 ggaaggcagt ggaggccgac aatgctgttt tgtaaacagc aagttcaggg ttcgagtccc
     1681 tgggtcggct ccgtgaataa ccgcagtaca gcaaggaaaa tgcggcgtcc gcaccggcga
     1741 ggacgccgcc tttgggttgg tgtgtagaca agaatgtaga caaactcctc gcgcgacgcg
     1801 gctggcccgg ctggtgcagc gggcctagcg ctggcgtgag cggcgggtgg ccgccttgcc
     1861 cgtgtgccgt ggatcgccca cggggccggc tggtgtgcag gaccgggagt aagccgactg
     1921 cggcagcgcg ttggcgcacc ggcagaagtg tcattcatca cggtatagtg cacggtagtc
     1981 atcggccata gcgcacaatc ggcggcaata ctgcacaggt tgggccgacc gctcgccacg
     2041 ccgcgtggct atcctctcag cagtgcgtga atcgtgactg ctgggaggaa gtatggcggg
     2101 aaggagcatc gatctgatgg acgtgcgcga gatcctccgc tacctacaga cgactaccaa
     2161 tctgagcgcg attcagcggg cgacggggtt gaaccggcgg acgctgatgc gctaccgggc
     2221 gtgggcgatg gaacatgggc tgctgacacg tccgctgcca tccttggaag agttgcagca
     2281 gctggtggcg cggaccctca cgccgccggc accgccgcag acggtctcga ccgtcgagcc
     2341 ctaccgcgac ctggtgcgcg acctgcacgc ccgtggcgtg gaggggacgg cgatctggca
     2401 gcgcctcgcc gagcagggct acacggggag tttgtcctcc gtctatcgct tcctccacca
     2461 gctcagccca ccggtgctgg gggtgacggt gcgggtggag cgggcgccgg gcagcgaggc
     2521 gcaggtcgac ttcggctacg ccggctacct gctggacgac acggccgggc ggcgccgcaa
     2581 ggcctgggcc ttcgtgatga cgctggccta cagccgccac cagtacgtcg agttcgcctg
     2641 ggaccagacg ctgccgacct ggatcgccct ccatcggcac gccttcacct tcttcggcgg
     2701 ggtgccgcag cgcgtcgtcc tggataatct gaaggcgggt gtcgccaggg cctgcttcga
     2761 cgacccgcag atccaggcga cctaccgcga gtgcgccgag cattacggct tcctcgtggc
     2821 cccctgcgcg ccgcggacac ccgagcataa ggggaaggtg gagcagggcg gcgtccacta
     2881 cgtcaagcgc aacttcctcg gcggccggcg ccccaccacg ctggcgcggg ccaacgcgga
     2941 tgtccgcgcc tggtgcgaga cgactgccgg gcggcgccgt cacggcacca cgaaagaggc
     3001 tccgctcgag cggttcgccg cagtggagcg ggaccagcta ctgccgctgc cgccggaatc
     3061 ctatgacttg gcgatctgga aggaggtcac gctccatcgc gactgccacg tcgtcttcga
     3121 gcaggccttc tactcggcgc cgttccgcct ggtcgggcag cgcctgcggg tgcgcggcgg
     3181 cagccgggag gtgcgcctgt acacgaccga ctaccaactg gtggccaccc acccacgcgc
     3241 gccggggccg ggcacgcggc agacccaccc cgaccacctg ccggcggcga aactgccaca
     3301 gttattgtgg gcccgggagc tgtgccgagt gctcgcggtc gaggtcggcc cggcggcgac
     3361 ggcggtggtg cagcagctcc tcgccgaccc ggtggtggac cgccacagcc gcgtcgtgcg
     3421 catcctgcgc ctgcgggaga cggtcggcga cgcgcgcctc gaggcggcct gcgcgcgggc
     3481 gctgcggtac gacgacctga cctacgccac catcaagcgc atcctggctc agggcctgga
     3541 ggcagcggag cgggacccga ctccgaccgt cgccccggcg cgcaccttcg tgcgcacggc
     3601 ggcggactta ctgggccatc tgttcggagg agtggcatgg acctccagca ccaactaagc
     3661 cccaagctca agcaactgcg cctttccggg gtgctcgcca ccctggaggc ccgccaccgg
     3721 caggcgctcg acggccagtg gtcctatgtt gagttcctgg agcggctgct ggaagatgag
     3781 gtggagcgcc gcgcccagaa gcaactcgcg ctgcgcatcc gccgcggcag cctgaacacc
     3841 accaagaccc tggagggcta cgacttcagc ttcaacccgg cggccagccg cccgcaggtg
     3901 ctccacctgg ccgctggcga ctacatccgc cgccggcgca acctgcttat ctgcggtccg
     3961 acaggtgtcg ggaagagtca tttggcgcaa gcgttggccc acgaggcggc gcggcagggg
     4021 cacgaggtgc tcttcgtcac caccgacaag atgctccacc atctccacgg cggccgcgcc
     4081 gacggcacct gggagcggcg gctggcgcag tacctgcgcc cggacctgct ggtgctcgac
     4141 gacttcggcc tcaagcccct gaccgacccg gccccgagcg atttgtacga cgtgattaat
     4201 gagcggtacg aggtcggcag catcctggtg accagtaacc gcgccccagc ggagtggccc
     4261 gacctcttcg gtgacccgct gctggcctcg gccgggctgg atcggctggc gcaccaggcc
     4321 gagacgctca tcatcaccgg gcgcagctac cgcgcccagg gacgagccgg cgtaggggag
     4381 gaacgaggat tgcttaaaga ggaaggaggt gggccaacgc cggaacactg agccggcgtg
     4441 ggcggccagg ccagcggtgt gcaggatcgc cgccgattcc tgtgcgcgat tgccgccgat
     4501 cgccgtgcag tcacgggcgg tgattgacag cagaaggggt aggaccaggt ggcgcgcggt
     4561 caggcgactt ggaggagcgc ctgcacctgg gtcaggaacg cgtcgaggtc gaacggctgg
     4621 gccagcagcc ccgcaaagcc ccgcgcggcg tagcccgcga accgcgacgg cgcgtacgcc
     4681 gtgaagatga cgacctgcat ggcccccgcg gcctgccgca gccacgccgg gctggtccac
     4741 gccgcggtgg tggtcggcgc gtctgccatc tcggccaggg tggcgaccgc caccacgcga
     4801 tagcccgcct cctgcagcgc caggacagtg acgtcccgga cggccgggtc gtcttccacc
     4861 aggagaatca gcgggcgagt atgaggagag cacagcatcg ggcgctcctt gccggtggat
     4921 gacggtatcc gccacccaca cgacgctagt gtgcctgatg catgcctggc caccactacc
     4981 gccaaggcgc accagccagc gtcaggcgct gacgagcagg cggtcgaccg tggccacgag
     5041 ctcatcgaga tcaaagggtt tggccaggaa cgccgtctgg acggggagcc ggggcggcgg
     5101 ccccgcactg agcagcagca ccgggggccc ggcatcagcc gcccgcgccc gcaactgggc
     5161 taccaagtcc acgccggaca gccggggcat catgatgtcg ctgataatca gagcggggta
     5221 ggcttgcgcg gccagcgcga gcgcctgggc accatcgtac gcggtcagca cggtatagcc
     5281 ttcatcagcc aggagatccg cgatggaggg gacatcgtca gcgaccagaa tcgtgggggc
     5341 catcggcctg cctagcgcca gtgtctggct tcatggcacc aaccaccacc tggcaggcga
     5401 cgtcgcatca cgcatgccag cgctgccgtg gcgtgcggcc agcatgcggc tctggcacgg
     5461 caatgtgtca gggccgcgag gaacgcggtc gcgtccccca ggagagatga gcggggccac
     5521 caggcccacg gcaccagtcg cagcaggcgt gccaccagca gaccacgccg tcaccggcgc
     5581 ggtggcggca ccactggtgt gtcccaccgg tcggcgggat agcccccgcc cggtgggata
     5641 gcccccgccc ggtggggcac cgcgcctgca ggtatgcgct cggagtctgc ggccagggcc
     5701 gccaccagag cgctgcactg gctggtgctg ttcgtggcct gctcgcggag gcgacacgcg
     5761 tgtcggaccg gcgcgatggt gccctgccta gctggcggtc gcggcgggcg ctggggcgct
     5821 gacctgacgc ctcacacggc cgctggctgg tgctgctgca gcgagcgacg gcacgcggcc
     5881 cgcgccggca aggagcagcc gagaggatct gctcagtgag acagccacgc caccggcgtg
     5941 ccagcaggag tatgtggtgc cgtgcg
//
LOCUS       DASUBI010000387         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_622826, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000387 DASUBI010000000
VERSION     DASUBI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622826"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1017
                     /locus_tag="VFU72_07490"
     CDS             46..1017
                     /locus_tag="VFU72_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5423364.1"
                     /translation="MPFAFTPTPLEEMPRLRAALGPAAPRLLMTRDDLTGFAFGGNKV
                     RALEFRMAHVLAHGYDAFVLVNIGQSNHARLHAAACTRLGIKMVIVKPGAKQEVAQGN
                     LLLDRMVGVEIVETGTTDPVALEQALDSVLADLRRRGHKPFASTREPFSELAGTIAYT
                     EAAIELTEQLQAAGVERAEIFLVGGTSAAGLALGGKLLGSPYRVQLISVGGSAEALRA
                     RTIRLTARVADILEVAPVITPDDFEVSDDYVGARYADVTPAVREAIRLAARTDAIFLD
                     PVYTGKSMAGLLGEIRKGRLTAEQTAVFVHTGGLPIIFAYSDLLKDL"
     gene            1119..2708
                     /locus_tag="VFU72_07495"
     CDS             1119..2708
                     /locus_tag="VFU72_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU5423365.1"
                     /translation="MTGRRGMVTSAHPVASLAGLRTLMEGGNAIDAAVAVASCIGATE
                     IGASGVGGVGWMLIYHAASGTLTTLDYQGWSPYATDRSRYRAPEDNYYGLLAAMVPGS
                     AMGWCAALERFGTMARADVFKHVIDICEQGFPLTVRNAQALTAAMGTPLFSYPSSRDF
                     YFPDGCAPRPGQLHKQPDLAGTFRALAEDGPDVYYHGHLADTIAGFCQENGGLITKRD
                     LADMTVEWVEPLDIRYRDYTIYGPPPPSSAMQWMQTLKMLESDDLVALGHNSADYLHT
                     LIEVERLAIVDRMHYNVQPEAPIGALLADDYCAARRALVNPARASKIVGERYARTTEV
                     GLRWHGSPLLRESTTHFDVVDAAGNAVACTQSLGGFGCGVVVPGTGIMLNNFMHWFDL
                     DGDSPNMIGPHKKNEMCLSPGMIWRDGRLLSCIGTPGSYGIMETTPQFMINLLDFGMT
                     VQAAIEAPRVRPQEMNRVIAEARIAPEVRTTLVERGHAVDVQPAWSALFGGAQGIVVD
                     PDSGTFTGGADPRRDGYAVGW"
     gene            2727..>3171
                     /locus_tag="VFU72_07500"
     CDS             2727..>3171
                     /locus_tag="VFU72_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5423366.1"
                     /translation="MTIGTIRGDFPALAGWIYLNCGGMAPLPQPVGEALLRIPRAVVR
                     EGPLRLQAHDEEFLGSEAARAIIARFIGAVPDEIAFTTQFSTAINIVIEGLPWRAGDE
                     IVVTDQEHPSLLAPVLNMARRRGLVVRRFAVAPSADELLTGLRRVL"
BASE COUNT          508 a         1062 c         1063 g          538 t
ORIGIN      
        1 ctgacgccca ccgagatcca tcagcgcatc gatcgtctac cgcggattcc gttcgccttc
       61 acacccacgc cgctcgagga gatgccccgg ctgcgcgcgg ccctcggtcc cgccgcgccg
      121 cgccttctga tgacgcgcga cgatctgacg ggttttgcgt tcggcgggaa caaggtccgc
      181 gcgctggaat ttcgcatggc gcacgtactg gcgcacggct atgacgcctt cgtgctggtc
      241 aacatcggtc aatccaatca cgcgcgcctc catgccgccg cctgcacccg cctgggcatc
      301 aagatggtta tcgtcaaacc cggtgcgaaa caggaggtcg cccagggcaa cctcctcctc
      361 gaccgcatgg tcggtgtcga gattgtcgag accgggacca ccgatccggt cgcgctggag
      421 caggccctcg actcggtcct ggccgatctc cggcggcggg ggcataagcc gttcgcctcg
      481 acccgcgagc ctttctccga actggccggc acgatcgcct acacagaggc ggcgatcgag
      541 ctcaccgagc agctgcaagc cgccggtgtg gagcgagccg agatctttct ggttgggggc
      601 acctcagcag cagggctcgc cctgggcggt aagctgctgg gcagcccgta ccgcgtgcag
      661 ctcatcagcg tcggcggctc agcagaagcc ctgcgcgcgc gcacgatccg gctcacggcg
      721 cgggtggccg acatcctgga ggttgcccct gttatcacgc ctgacgactt cgaggtgtcc
      781 gacgactacg tcggcgcgcg ctatgccgat gtgacgccgg ccgtgcgaga ggcgattcgc
      841 ctggccgctc gcaccgacgc gatcttcctt gacccggtct atacagggaa gtcgatggcg
      901 ggcctgctcg gtgagatccg gaaagggcgg ctcacggccg agcagaccgc cgtcttcgtg
      961 cataccgggg gcttgccgat catcttcgcc tacagtgacc tgctgaaaga tctgtagcag
     1021 ggtgcctccg aatcgctcga cggtcgccaa ttcaaacaca gaaaggacgt aggtcagcga
     1081 tgagtctttc ttatggcggc gttcagcatc gcccggccgt gaccggccgg cgcggtatgg
     1141 tgacctcggc ccatcccgtt gcctctctgg ccggcttgcg caccctcatg gagggcggca
     1201 acgccatcga tgcagcggtc gccgtcgcct cctgcatcgg cgcgaccgaa atcggggcct
     1261 cgggcgtcgg cggcgtgggt tggatgctga tctatcacgc cgcgtcgggc acactgacca
     1321 cgctcgacta ccagggctgg agcccgtatg cgaccgaccg gtcccgctac cgcgcgccgg
     1381 aggacaacta ttacggcctg ctggccgcga tggtgccggg gtcggctatg ggctggtgcg
     1441 ccgcgctgga acggttcggc acgatggcgc gtgccgacgt cttcaagcat gttatcgaca
     1501 tctgcgaaca gggcttcccg ctcaccgtcc gcaacgccca ggcgctgacc gccgcgatgg
     1561 gaaccccact cttcagttat ccctcgtcgc gcgacttcta cttccccgac gggtgcgccc
     1621 ccaggccggg tcagctgcac aagcagccgg accttgccgg gaccttccgg gcgctggcgg
     1681 aggacggccc cgacgtgtac tatcacgggc acctcgccga caccatcgcc ggtttctgcc
     1741 aggagaacgg cggattgatc acgaagcgcg acctggccga catgaccgtc gagtgggttg
     1801 agccactgga catccgctac cgcgactaca ccatctatgg ccccccgccg ccatccagcg
     1861 ccatgcagtg gatgcagacg ctgaagatgc tggaaagtga cgacctggtg gcgctgggcc
     1921 acaacagcgc cgactacctg cacaccctga tcgaggtcga gcgcctggcg atcgtcgatc
     1981 ggatgcacta caacgtccag cccgaggctc cgatcggcgc gctgctggcc gatgactact
     2041 gcgcggcgcg ccgggcgctg gtcaatccgg ctcgggcgag caagatcgtc ggcgaacgct
     2101 acgcgcgcac caccgaggtg ggattgcgct ggcacggcag ccctctgctg cgcgagagta
     2161 cgacccactt cgacgtcgtg gacgccgcgg gcaacgccgt cgcctgcacc cagagcctgg
     2221 gcggcttcgg ttgcggcgtg gtcgtgcccg gcaccgggat catgctgaac aactttatgc
     2281 actggttcga tctcgatggc gacagcccga atatgatcgg gccgcacaag aagaacgaaa
     2341 tgtgcctgtc gcccgggatg atctggcggg acggacgcct cctctcctgc atcgggacac
     2401 ctggctccta cggcatcatg gagacgacgc cgcagttcat gatcaatctg cttgactttg
     2461 ggatgacggt gcaggcggcg atcgaggcgc cgcgcgtccg cccgcaggag atgaaccgcg
     2521 tgattgcaga ggcgcgcatt gcgccggaag tgcgcaccac actcgtggag cgcggacacg
     2581 cggtggacgt ccagccggcg tggtcggcac tcttcggcgg ggcgcaaggt atcgtggttg
     2641 accccgacag cgggacgttc acgggcggcg ccgatccccg tcgcgatggc tacgcggtgg
     2701 gctggtagcg cgggaagggg agatcggtga cgatcggcac gattcgcggc gactttccgg
     2761 cgctcgccgg ctggatctac ctcaactgcg gcggtatggc gccgctgcca cagccggtcg
     2821 gagaggcgct gctgcgtatc cctcgagcgg tggtccgcga gggtccgctg cgcttgcagg
     2881 cgcacgacga ggagtttctc ggcagcgagg cggcacgggc aatcatcgcc cgcttcatcg
     2941 gcgccgtgcc cgacgagatt gccttcacga cccagttctc gacagcgatc aatatcgtca
     3001 tcgagggttt gccctggcgt gcgggtgatg agattgtcgt cactgaccag gaacacccgt
     3061 cgctgctggc tccggtgctg aatatggcgc ggcggcgcgg gctggtggtg cggcgcttcg
     3121 cggtcgcgcc gagcgccgat gagctgctga ccggcttgcg ccgcgtgctg a
//
LOCUS       DASUBI010000388         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_166034, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000388 DASUBI010000000
VERSION     DASUBI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166034"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3211
                     /locus_tag="VFU72_07505"
     CDS             <1..>3211
                     /locus_tag="VFU72_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU5423367.1"
                     /translation="LAKRGVRLGVLVGTAYLFTEEAVRGGAITDAFQQAALACEQTVL
                     LESGPGHATRCLPSPFAGDFAEEKRRLIQRGLPSEEIRGELEVLNIGRLRIASKGVDR
                     HPRYGEDPSVPKLVELGGQEQWARGMYMIGQVASLQSQVRTIAALHADVSLNGTAALE
                     DLAPERDEEPAPLPPADIAVVGIGTIMPGASDLQTFWDNIVNKVDAVREVPRERWDWH
                     HYFDPDRNARDKSYSRWGGFIDPVPFDPVSFGMPPNSLKSIEPFQLLGLLVTQAALRD
                     AGMLDRPFDRERTSVILGAGGGGADLSSNYVVRSSLPGLFGERAPELADGLSAALPEW
                     TEDSFPGILMNVAAGRIANRFNFGGVNYTIDAACASSLAAVYQGVRELQAGTSEVAIV
                     GGVDAIQNPFSYMCFSKTQALSPTGRCRPFDAAADGIAISEGFAALVLKRLADAERDG
                     DRIYAVIRGAGGSSDGRDRSLTAPRPEGQIRALRRAYRQAGFSPATVELVEAHGTGTV
                     AGDRAEVSSLSTFLGAAGAAAQNCAIGSVKSMVGHTKAAAGVAGLAKVALALHHRVLP
                     ATLGVSQPNASVNFPNGPIYVNTESRPWVHGAPDYPRRAGVSAFGFGGTNFHLVLEEY
                     TGNFLPDQSLGLSDWPAELFVWRAHSRDEILAGVTRLATALERGARPPLADLAYTLAR
                     QAGTVEASAATLALVAGSWDELAEKLRGARDFLATGAGRQHLPTGVHFSAVPLAAEWQ
                     IAFLFPGQGSQYVNMARELAVVFPEVRAVFDAADQALDGRFEQPLSRLIFPPPVFAES
                     DAKRQQAELTETNVAQPALGATGLAYLHLLRGLGVTPQMTAGHSYGEFVALHAAGCFD
                     AATLLALSEARGRFMREGASEESGTMAAVSAAPEALQPLLADPELTLANLNHPTQTVV
                     SGTHAAVERALAWCQEQGLRAQLLPVACAFHSPLVAPAQQRLAELLGNVAIAPPQMPV
                     YSNTIAAPYPDDGEQMVALLAEHLVRPVEFVRQVRAMYAAGARVFVEVGPKSVLSGLV
                     GRILGDEPHLCVPLDQAGRPSLVTLLYGLAALISEGTPVR"
BASE COUNT          427 a         1196 c         1126 g          462 t
ORIGIN      
        1 gctggccaag cgcggcgtgc gcctcggtgt gctggtcggc actgcctacc tctttaccga
       61 ggaggccgtg cgcggcggcg ccatcaccga cgccttccag caggcggcgc tggcttgcga
      121 gcaaaccgtg ctgctggaga gcggccccgg ccacgctacc cgctgcctgc ccagcccctt
      181 cgccggcgac ttcgccgagg agaagcgccg cctgatccag cgcggcctgc cctccgagga
      241 gatccgcggc gagctggagg tgctcaacat cgggcgccta cgcatcgcct cgaagggcgt
      301 cgaccgccac ccgcgctacg gcgaggaccc cagcgtgccc aagctggtcg aacttggcgg
      361 ccaagagcaa tgggcgcgcg gcatgtacat gatcggccag gtggcctcgc tgcagtccca
      421 ggtgcgcacc atcgccgcgc tgcacgccga tgtctcgctc aacggcaccg ccgcgctgga
      481 ggatctggcg ccggagcggg acgaggagcc ggcgcccctg ccgccggccg atatcgcggt
      541 cgtcggcatc ggcacgatca tgcccggcgc cagcgacctg cagaccttct gggacaacat
      601 cgtcaacaag gtcgatgccg tgcgcgaggt gccgcgcgag cgctgggact ggcaccacta
      661 cttcgacccc gaccgcaacg cgcgcgacaa gtcctactcg cgctggggcg gcttcatcga
      721 cccggtgccg ttcgacccgg tcagcttcgg catgccgccg aacagcctga agtcgatcga
      781 gccatttcaa ttgctggggc tgctggtcac gcaggccgcg ctgcgggacg ccggcatgct
      841 cgaccgcccc ttcgaccgcg agcgcacctc ggtcatcctc ggcgcgggcg gcggcggcgc
      901 cgatctcagc agcaactacg tggtccgctc cagcctgcct ggcctcttcg gagagcgagc
      961 gccggagctg gccgacggcc tgagcgcggc gctgccggag tggaccgagg actccttccc
     1021 aggcattctg atgaacgtgg ccgccgggcg catcgccaac cgcttcaatt tcggcggggt
     1081 caactacacc atcgacgcgg cctgcgcctc atcgctggcc gcggtctacc agggggtgcg
     1141 cgagctgcag gccggcacca gcgaggtcgc catcgtcggc ggggtggacg cgatccagaa
     1201 ccccttctcc tacatgtgct tcagtaagac ccaggcgctc tcgcccaccg gccgctgccg
     1261 gcccttcgac gccgccgccg acggcatcgc catcagcgag ggcttcgccg cgctcgtgct
     1321 caagcgcctg gccgacgccg agcgcgacgg cgaccgcatc tacgccgtga tccgcggcgc
     1381 gggtggctcc agcgacggcc gcgaccgcag cctgaccgcg ccccggccgg agggccagat
     1441 ccgcgccctg cgccgcgcct accgccaggc cgggttctcg ccggcgacag tcgagctggt
     1501 cgaagcgcac ggcaccggca cggtggccgg cgaccgcgcc gaggtctcct cgctctcgac
     1561 cttccttggc gcggccggcg ccgccgcgca gaactgcgcc atcggctcgg tcaagtcgat
     1621 ggtcggccac accaaggccg ccgccggcgt ggccgggttg gccaaggtgg cgctggcgct
     1681 gcaccaccgc gtgctgccgg cgacgctcgg cgtcagccag cccaatgcca gcgtcaactt
     1741 ccccaatggt ccgatttatg tcaataccga gtcacgtccc tgggtccacg gtgcgccgga
     1801 ctacccgcgc cgggcgggcg tcagcgcctt cggcttcggc ggcaccaact tccacctggt
     1861 gctggaggag tacaccggca acttcctgcc cgaccagagc ctggggttga gcgactggcc
     1921 ggccgagttg ttcgtctggc gggcgcactc ccgcgacgag atcctggccg gcgtgacgcg
     1981 cctggccacg gcgctggagc gcggcgcgcg cccaccgctg gccgatctgg cctacaccct
     2041 ggcccgccag gcgggcacgg tggaggcgag cgccgcgacg ctggcgctcg tggccggctc
     2101 ctgggatgag ctagccgaga agctgcgcgg ggcgcgcgac tttctggcga cgggcgccgg
     2161 ccggcagcac ctgccgaccg gggtgcactt ctccgcggtg ccgctggccg ccgagtggca
     2221 gatcgccttc ctcttccccg gccagggctc gcagtacgtc aacatggcgc gcgaattggc
     2281 ggtcgtcttc cccgaggtcc gcgccgtctt cgacgccgcc gaccaggcgc tcgacggccg
     2341 cttcgagcag ccgctcagcc gcctgatctt cccgccgccg gtcttcgccg agtccgacgc
     2401 caagcgccag caagctgagc tgaccgagac caacgtggcg cagccggcgc tgggcgcgac
     2461 cggcctcgcc tacctgcacc tgctgcgcgg tctcggcgtc acgccgcaga tgaccgccgg
     2521 ccacagctat ggcgagttcg tggcgctgca cgccgcgggc tgcttcgacg ccgccacgct
     2581 cctggcgctc tcggaggcgc gcgggcgctt catgcgcgag ggcgcctcgg aggagagcgg
     2641 cactatggcg gcggtcagcg ccgccccgga ggcgctgcag ccgctgctgg cggacccgga
     2701 gctcacgctg gccaatctca atcatccgac gcagacggtg gtctcgggca cgcacgcggc
     2761 cgtcgagcgg gcgctggcct ggtgccagga gcagggcctg cgcgcccagc tcctgccggt
     2821 cgcctgcgcc ttccactcgc cgctggtggc tccggcccag cagcggctgg ccgagctgct
     2881 ggggaacgtc gcgatcgctc cgccgcagat gccggtctat tcgaacacga tcgccgcgcc
     2941 ctacccggac gacggcgagc agatggtcgc gctgctggcc gagcatttgg ttcggccggt
     3001 ggagttcgtg cgccaggtgc gggccatgta cgcggccggg gcgcgcgtct ttgtggaggt
     3061 gggaccaaag agcgtgctaa gcggcctggt cggccgcatc ctgggcgatg agccacatct
     3121 ctgcgtcccg ctcgatcagg cgggtcgccc ctcgctggtg acgctgctgt acgggctggc
     3181 cgcgctgatc agcgagggta cgccggtgcg g
//
LOCUS       DASUBI010000389         7416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_164557, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000389 DASUBI010000000
VERSION     DASUBI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164557"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFU72_07510"
     CDS             <1..500
                     /locus_tag="VFU72_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5423368.1"
                     /translation="FHPLGTDQPVEAGMLMKTDFGGYRDGYYSDVARTVCLGRASDQQ
                     RDTYARLQEIKHRTVDFIKPGVLASEVANYTRDLYRQQGLEYKWAIIGHGIGLGIHES
                     PQIYPWVHDPILPGMVMMIELGYTDYPHDSYHIEDLIVVTERGAEYRSDFSRHERLWE
                     VGMEG"
     gene            complement(497..889)
                     /locus_tag="VFU72_07515"
     CDS             complement(497..889)
                     /locus_tag="VFU72_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423369.1"
                     /translation="MPHDPLIAVVNDDTAFLNMMNELLSDEGYRTVIWREGEGAYDLV
                     CQHRPSLVILDIRMEHPGTGWHVLELLRLDPETASIPVIVCSADSRLLRAKQDRLQSL
                     NCQTLEKPFDLDELLAMVRLALGQDPQD"
     gene            1071..1871
                     /locus_tag="VFU72_07520"
     CDS             1071..1871
                     /locus_tag="VFU72_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5423370.1"
                     /translation="MAGRCIFLLDAGAGAPLLFLHGIPTFSYLWREVVPPLAESRRCL
                     APDLLGFGYSEKPSGTSFGPARQAEMVTGLLDELGIDRVAVVAHDFGALVAAELLARW
                     PERVAALVLTNTSLRRDQWRSGPPFSLLRLPLVGELAMALARRWMLTLAMRVYVQNDT
                     RLTRPVMAHYWWPFQHGFKRTLLALSRERWAGEDDFGRWRAALAAFAAPCLLVWGARD
                     PTFTLAEANDLAALLPRGQLEVLPDANHFLQEDAPAALAALIDAFLSA"
     gene            complement(1880..4621)
                     /locus_tag="VFU72_07525"
     CDS             complement(1880..4621)
                     /locus_tag="VFU72_07525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5423371.1"
                     /translation="MREMPRAARLYLAGCYLLGLAALVWLTCLASWQADERSRLLTAA
                     LAVLAATAQVFTVARAQGHHSDHLTPAPIFAAVLLLPAPFLALVVCFTFIPEWIVHRR
                     RWFIQLFNIASWLIAAAGAREVIHALAGRPELARQGPLNAGVILLALAVFLALSTFLL
                     AWVLRLARRQSLRETGLFVPAKLFSEIALLCSGWAFAAAWAVDPYYAIPAVLPLLVIF
                     QALHVPNLIVEASTDPKTGLANMRHVNAVLPRELERAERTGQPGSVLMCDLDYLRTIN
                     NTYGHPVGDIVLAGVADVIRQNVRACDLAGRFGGEEFIIYLLDTPQEGAWQVGERIRG
                     ELEHRRFPTGEGNQTVGATVSIGLASYPLDGSSYETLLREADLALYQAKREGRNRVIA
                     AGRASRELVGDWARDNVLPAQRDPGSTGRLPFIQFGSQLLRTRPPAPVGQAAEPPVQE
                     QSPRPLPAAPVRQGLLGIFIAYCLAAALALLLPGLAGLLHSHIPWLGLLFFASLVIVA
                     EQLSIDLDGRSQTSVSVVAILATAFLYGRLGILVVAAIFALWAKLKSHSPLHRMLFNF
                     AMIALAATVANAGFRLLAREPLDGAGFARLALLAAASGLIYYLINHALLSTVRGLNEG
                     RSVVAIFRADYQWLWPYYAVFGLLGLTVAEAYIAFGWAGVLAIAAPVAMVRFSIKQYM
                     ERTAVYVNELKLVNQRLTDSYEATLQALSRALDTRDAETEEHSQRVRRYSQLIARRCG
                     LSEEEIEHLCRGALLHDIGKIGVPDAVLLKRGRLTEPETELMRRHPTIGFTMIAHIPF
                     LTKAAEVVLHHHESFDGSGYPSGLSAHRIPLVARIFTVVDALDAMTSNRPYRQALPFT
                     QALDELRRCSGTQFDPHIVDIVLAIPLDELIACSRGDLENLPATYLSDADGLAIASD"
     gene            complement(4822..5256)
                     /locus_tag="VFU72_07530"
     CDS             complement(4822..5256)
                     /locus_tag="VFU72_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423372.1"
                     /translation="MSDELTGQPAGGARIRRREEMPALERGKGARSWLFLDREQGAEH
                     FIAGSSTFQPGIEVPRHSHNVEEFVTVLEGAGECEIEGASYPVKPFDTTFIPAGLVHC
                     FRNTGDTPLTILWIYGGTEVTRTFAGTGITVRHFSPEDRVGR"
     gene            complement(5253..6158)
                     /gene="rfbA"
                     /locus_tag="VFU72_07535"
     CDS             complement(5253..6158)
                     /gene="rfbA"
                     /locus_tag="VFU72_07535"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU5423373.1"
                     /translation="MKGIILAGGTGSRLYPITQVVSKQLLPVYDKPMIYYPLTTLMLA
                     GIQEILIISTPDDTPRFARLLGDGAQWGVDFHYAVQERPEGLAQAFMVGRDFVGRDPV
                     ALILGDNIFYGHELAERVQRAARRTDGASIFAYYVQDPQRYGVVEFDRAGLVVSLEEK
                     PARPRSSYAVTGLYFYDNDVLDIAAGLRPSARGEYEITDVNLAYLRRQRLRVEVLGRG
                     TAWLDTGTHESLQQAASFIQIIEQRQGLKIACPEEVAYRMGYIGPEAVTALADGLPNG
                     YGQYLRRLLEDPTVRPSQEWMRSRT"
     gene            complement(6155..7207)
                     /gene="rfbB"
                     /locus_tag="VFU72_07540"
     CDS             complement(6155..7207)
                     /gene="rfbB"
                     /locus_tag="VFU72_07540"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5423374.1"
                     /translation="MRLLVTGGAGFIGSALVRHVVRELGAPLLNLDKLTYAGNLNAVA
                     EVAGHPAYHFAQVDLADAPAVQAAVASFAPTAIVHLAAETHVDRSIDDPGVFIQSNIV
                     GTFNLLEAAHAYWRALPEDERAAFRLLHVSSDEVYGALGADGAFSERSPYQPNSPYSA
                     SKAAADHLVRAWHHTYGLPALITNCSNNYGPYQYPEKLIPLMIRTALAGQPLPVYGQG
                     RQVRDWLYVEDHVRALLTVLERGTPGETYVVGGRNERPNIEVVEAICELLDELRPDAP
                     QAPHRQLISFVPDRPGHDQRYAIDPGKLMDELGWRPREGFQSGLLKTVRWYLEHPEWA
                     REALSAGRLGLGWARA"
BASE COUNT         1170 a         2434 c         2684 g         1128 t
ORIGIN      
        1 acttccaccc gctcggcacc gaccagccgg tcgaggccgg gatgctgatg aagaccgatt
       61 tcggcggcta tcgagacggc tattattcgg acgtggcgcg caccgtctgc ctcggccgcg
      121 ccagcgacca acagcgggac acctacgcgc gcctgcagga gatcaagcac cgcaccgtcg
      181 acttcatcaa gcccggcgtg ctggcctcgg aggtcgccaa ctacacacgc gatctctatc
      241 ggcagcaggg gctggagtac aagtgggcga tcatcgggca cggtatcggc ctgggcatcc
      301 acgagtcgcc gcagatctac ccctgggtcc acgatccgat cctgccgggg atggtgatga
      361 tgatcgagct ggggtacacc gactacccgc acgatagcta ccacatcgag gatctgatcg
      421 tggtgaccga gcggggcgcc gagtatcgct cagacttcag ccgccacgag cggctgtggg
      481 aggtggggat ggaaggctag tcctgggggt cttgccccag cgccaggcgg accatcgcca
      541 gcagctcgtc gaggtcgaag ggcttctcca gcgtctggca gttgagactc tgcagccggt
      601 cctgcttcgc gcgcagcagg cggctgtcgg ccgagcagac gatcaccggg atggaggcag
      661 tctcggggtc gagccgcagc aactccagca catgccagcc ggtgccgggg tgctccatgc
      721 ggatgtcgag aataacgagg ctgggccggt gctggcagac cagatcgtag gcgccctcgc
      781 cctcgcgcca gatcaccgtg cggtagccct cgtcgctgag aagctcgttc atcatattga
      841 gaaaggcggt gtcgtcgttg accaccgcga tgaggggatc gtgcggcacc tggttgctcc
      901 cttctcgggc gcgtgcccgc gacgcgcgcg ctacgtgccc cgtccagttc cgtctgcgtt
      961 agatgcaaag cgcgtacccg gacaatttgc agcctgatct cagggcgcgg tgcgccgagc
     1021 gaaccggcgc cggtggtggc gccttccggg cttccgggcg ctacctcgac gtggccggac
     1081 gctgcatctt tctgctcgat gcgggcgccg gggcgccgct cctcttcctg cacggcatcc
     1141 cgaccttctc ctatctctgg cgcgaggtgg tcccgcccct ggcggagtcg cggcgctgcc
     1201 tggcgccgga cctgctcggc ttcggctact cagagaagcc aagcggcacg agcttcggcc
     1261 ccgcccggca ggccgagatg gtgacggggc tgctcgatga actggggatc gatcgcgtcg
     1321 ccgtggtcgc gcacgacttc ggcgcgctgg tggcggcgga gctgctagcg cgttggccgg
     1381 aacgagtcgc cgcgctggtg ctgaccaata ccagcctgcg gcgggatcag tggcgcagcg
     1441 ggccgccgtt ctcgctgctg cgcttgccgc tcgtcggcga gctggccatg gcgctggcgc
     1501 ggcgctggat gctcacgctg gccatgcgcg tctacgtcca gaacgacacg cgcctgacgc
     1561 gcccggtgat ggcgcactac tggtggccct tccagcacgg cttcaagcgc acgctgctgg
     1621 ctctctcacg cgagcgctgg gccggcgagg acgacttcgg ccgctggcgc gcggcgctgg
     1681 cggcgttcgc cgcaccctgt ctgctggtct ggggcgcgcg cgacccgacg ttcaccctgg
     1741 ccgaagccaa cgacctggcg gcgctgctgc cgcgcgggca gctggaggtg ctgccggatg
     1801 ccaaccactt cctgcaagag gacgcgcccg ccgcgctggc ggcgttgatc gacgcctttc
     1861 tcagcgcctg agacggcgct cagtcggagg cgatggcgag accgtcggcg tccgagaggt
     1921 aggtagcggg caggttctcc aggtcacccc ggctacacgc gatcagctcg tcgagcggga
     1981 tggccagcac gatatcgacg atatgcggat cgaactgggt gccggagcag cggcggagct
     2041 cgtccagcgc ctgggtgaac gggagcgcct gacggtaggg ccggttggag gtcatggcgt
     2101 cgagggcatc gaccacggtg aagatgcgcg ccaccagtgg gatgcggtgc gccgacaggc
     2161 cgcttgggta gccgctgccg tcgaaggatt cgtggtggtg cagcacgacc tcggccgcct
     2221 tcgtcaggaa ggggatgtga gcgatcatgg tgaagccgat ggtcgggtgc cgccgcatca
     2281 gctcggtctc aggctcggtg agccggccgc gtttgaggag cacggcgtcg ggtacgccga
     2341 tcttgccgat gtcatggagc agcgcgccgc ggcaaaggtg ctcgatctcc tcctcggaga
     2401 gcccgcagcg ccgggcgatg agctgcgagt agcgtcgcac ccgttgggag tgctcttcgg
     2461 tctccgcatc gcgcgtatcg agcgcgcgcg acagcgcctg gagggtggcc tcgtaggagt
     2521 cggtcaggcg ctggttgacc agcttcagct cgttgacgta gaccgcggtg cgctccatgt
     2581 actgcttgat cgagaagcgc accatcgcga cgggcgcggc gatggccagc acgccggccc
     2641 agccgaaggc gatgtaggcc tcggccacgg tcagcccgag caagccgaag accgcgtagt
     2701 agggccagag ccactgatag tcggcgcgga agatggcgac gaccgaccgc ccctcgttca
     2761 ggccgcgcac ggtcgagagc agcgcgtggt tgatcaggta gtagatcaaa cccgacgcag
     2821 cggcgaggag cgccagccgc gcgaagccgg cgccgtcgag cggctctcgt gccaggaggc
     2881 ggaagccggc gttggccacg gtggcggcga gcgcgatcat ggcgaagttg aagagcatgc
     2941 ggtgcagcgg gctatgtgac ttgagcttgg cccacagcgc gaagattgct gccaccacca
     3001 aaatgccgag ccggccgtag aggaaggcgg tcgccaggat cgccaccacc gagacggagg
     3061 tctggctgcg gccgtcgagg tcgatcgaga gctgctcggc cacgatcacc agcgaggcaa
     3121 agaagaggag ccccagccag gggatgtggc tgtgcagcag cccggctaat cccggcagga
     3181 ggagagccag ggccgccgcc aggcaatagg cgatgaagat gcccagcaac ccctgccgga
     3241 ccggcgcagc tgggagcggc cgcggcgact gctcctgcac cggaggctcg gccgcctggc
     3301 cgacgggcgc gggcgggcgt gtccggagca gttgcgagcc gaactggatg aaggggaggc
     3361 gaccggtact gcccggatcg cgctgcgccg gcagaacgtt gtcgcgtgcc cagtcgccga
     3421 ccaactcccg actggcgcgc ccggcggcga tgacgcggtt gcggccctcg cgcttggcct
     3481 ggtagagcgc cagatccgcc tcgcgcagca acgtctcgta gctgctgcca tcgaggggat
     3541 agctcgccag accgatgctg accgtggcgc ctaccgtctg gttgccctcc ccggtcggga
     3601 aacggcggtg ctccagttcg ccgcgaatcc gctcgccgac ttgccaggcc ccttcctgcg
     3661 gggtgtccag caggtagatg atgaactctt cgccgccgaa acgcccggcc agatcgcagg
     3721 cacgcacgtt ttgccggatc acgtcggcca ccccggccag tacgatgtcg ccgaccgggt
     3781 ggccgtaggt gttgttgatc gtgcgcaggt agtcgagatc gcacatcaaa acgctgcccg
     3841 gctgcccggt gcgctcggcg cgctccagct cacgcggcag cacggcgttg acgtgccgca
     3901 tgttggccag accggtcttg ggatcggtgg aggcctcgac gatcagattg ggaacgtgga
     3961 gcgcctggaa gatgaccagc agcgggagca ccgccgggat ggcgtagtag ggatcgacgg
     4021 cccaggcggc ggcgaaggcc caccctgagc agaggagcgc gatctccgag aagagttttg
     4081 ctgggacgaa gagcccggtt tcgcgcagcg actgccgccg cgccaggcgc agtacccacg
     4141 cgagcaggaa ggtggagagc gccaggaaga ccgccagcgc cagcaggatg accccggcgt
     4201 tgagcggtcc ctgccgcgcc agctcgggcc ggccggccag ggcatgaatg acttcgcgtg
     4261 cgcccgcggc cgcgatcagc cacgaggcga tgttgaagag ctggatgaac cagcgccggc
     4321 gatggacgat ccattcgggg atgaaggtga agcagacgac cagggccagg aagggggcag
     4381 ggagcaacag cacggcggcg aagattggcg ccggcgtgag gtgatcggag tgatggccct
     4441 gcgcgcgcgc cacggtgaag acctgggcgg tggccgccag cacggccagc gcggcggtca
     4501 gcaaccggga ccgctcgtcg gcctgccagg acgcaaggca ggtcagccag accagcgcgg
     4561 ccaggccgag gaggtagcag ccggcgaggt agaggcgtgc ggcgcgcggc atctcacgca
     4621 tcgcgaactc ccggatgatg gctcgcggcc ggcgccccgc gcgcggctcg atcgcgagca
     4681 gccctgatgc cccgctcgca atttcacttt ccgggctacg ttcgtacaac cgattggggt
     4741 gggtaattgc tcccaggccg gcacggacgt tggactcatc gcggcgagac attcggcccg
     4801 cgggatgtct aacaggcggc tctaacgccc gacgcggtct tcgggcgaga agtgacgcac
     4861 ggtgatgccg gtgccggcga aggtgcgggt gacttcggtg ccgccgtaga tccagaggat
     4921 ggtaagtggt gtgtccccgg tgttgcggaa gcagtggacc aggccggccg ggatgaaggt
     4981 ggtgtcgaag ggtttgaccg ggtagctggc gccctcgatc tcgcactcgc cggcgccctc
     5041 caacaccgtc acgaactcct ccacgttgtg gctgtggcgc ggcacctcga tccccggctg
     5101 gaaggtcgag ctgccggcga tgaagtgctc ggcgccctgc tcgcggtcaa ggaacagcca
     5161 actgcgggcg cccttgccgc gctccagcgc cggcatctct tcgcgccgcc ggatgcgggc
     5221 acctccggcc ggttgccccg tcagctcatc gctcatgtgc ggctcctcat ccactcctgg
     5281 ctgggccgga cggtcgggtc ctccagcagg cggcggagat actgaccgta gccgttgggc
     5341 aggccgtcgg ccagggccgt caccgcctcc ggaccaatgt agcccatgcg gtaggcgacc
     5401 tcctcgggac aggcgatctt cagcccctgc cgctgctcga tgatctgaat gaagctcgcc
     5461 gcctgttgca gcgactcgtg cgtgccggtg tcgagccagg cggtgccgcg cccgagcact
     5521 tccacgcgca gccgctgccg ccgcaggtag gccaggttga cgtcggtgat ttcgtactcg
     5581 ccgcgcgccg agggacggag ccccgccgcg atgtcgagca cgtcattgtc gtagaagtag
     5641 aggccggtga cggcgtagga ggacctgggc cgtgccggtt tctcctccag gctgaccacc
     5701 agcccggcgc ggtcgaactc gaccacgccg tagcgctgcg ggtcctgcac gtagtaggcg
     5761 aagatgctgg cgccgtcggt ccggcgcgcg gcgcgctgca cgcgctcggc cagctcgtgg
     5821 ccatagaaga tgttgtcgcc caggatcagc gccaccgggt cgcgtccgac gaagtcgcgc
     5881 ccgaccatga aggcctgggc caggccctcg ggccgctcct gcacggcgta gtggaagtcc
     5941 acgccccact gggcgccgtc gcccagcagg cgcgcgaagc gcggcgtgtc gtcgggggtc
     6001 gagatgatca ggatctcctg aatgccggcc agcatgaggg tcgtcagcgg gtagtagatc
     6061 atcggcttgt catagaccgg cagcagttgc ttgctgacta cctgggtaat cgggtagagc
     6121 cggctgccgg tgccaccggc caggatgatc cccttcatgc ccgcgcccag cccagcccca
     6181 gccgcccggc ggacagcgcc tcgcgcgccc actcgggatg ctccaggtac cagcgcaccg
     6241 tcttgagcag gccgctctgg aagccctcgc gcggccgcca gcccagctcg tccatcagct
     6301 tgcccgggtc gatggcgtag cgctggtcgt ggccggggcg gtcgggcacg aagctgatca
     6361 gctgccggtg gggcgcctgc ggcgcgtcgg ggcgcagctc gtcgagcagc tcgcagatgg
     6421 cctccacgac ctcgatattg ggccgctcgt tgcgcccgcc gacgacgtag gtttcgcccg
     6481 gtgtgccccg ctccagcacg gtcagcaacg cgcgcacatg gtcttccacg tagagccaat
     6541 cgcgcacctg ccggccctgg ccgtagaccg gcagcggctg gccggccagc gcggtgcgga
     6601 tcatcagcgg gatcagcttc tcggggtact gataggggcc gtagttgttg gagcaattgg
     6661 tgatcagcgc cggcaggccg taggtgtggt gccaggcgcg caccagatgg tcggcggccg
     6721 ccttgctggc cgagtagggc gagttgggtt ggtagggcga gcgctcggaa aatgcgccgt
     6781 ccgccccgag cgcgccgtag acctcgtcgg aggagacgtg cagcaagcgg aaggccgcgc
     6841 gctcgtcctc tggcagcgcg cgccaatagg cgtgggccgc ctccaataag ttgaaggtgc
     6901 cgacgatgtt gctctgaatg aagacaccgg ggtcgtcgat cgagcggtcg acgtgcgtct
     6961 cggcggccag gtggacgatg gcggtgggcg cgaaggaggc gacggcggcc tgcacggccg
     7021 gcgcgtcggc cagatcgacc tgggcgaagt ggtaggccgg gtggccggcg acctccgcga
     7081 ccgcgttgag attgcccgcg taggttagct tgtcgagatt gagcagcggc gcgccgagct
     7141 cgcgcaccac gtggcgcacc agtgccgatc cgataaagcc cgcacctccg gtcaccagca
     7201 gccgcaccgc ggtaatccct tccccggtcc cacaccgctt ccgggctgat tatgcacgcg
     7261 cgccccggtg acaggccagc acaggcggct atcccaccag cggcgggcga cgccggccga
     7321 cctgggtggc ctgctcgaag gcgtgtgcca attgcagcac gccgaggtca tcatgcgtgc
     7381 ggccgacgat ctggatgccg accggcagcc cctccg
//
LOCUS       DASUBI010000390         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1231777, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000390 DASUBI010000000
VERSION     DASUBI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231777"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFU72_07545"
     CDS             complement(<1..348)
                     /locus_tag="VFU72_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5423375.1"
                     /translation="MSVNTELRAALAAIRGRTLLVVGDLYLDCYIFGHPARVSREAPI
                     MVLEETRREERPGGGTAPALAVRALGGEVWQVGVVGDDAEGRELRTLLRARGVNTDGV
                     LIDPSRPTTTKTRL"
     gene            complement(345..893)
                     /locus_tag="VFU72_07550"
     CDS             complement(345..893)
                     /locus_tag="VFU72_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEU5423376.1"
                     /translation="MVLSDIEEQRCTYHPNVRTRLRCSKCGTPICPRCAVETPVGFRC
                     PTCAAVRGLPTYSTGPATMVKAIVVGLVVALAVGILWGVIPDWRFYMALLLGFGVAEG
                     IAWAAQEKRGADLQLAGILLVIVGMVVARVVMAERSPFFTLDMLLHHATQPAISSFFQ
                     LRLLPDALFAALALLIPWIRFR"
     gene            complement(1028..1489)
                     /locus_tag="VFU72_07555"
     CDS             complement(1028..1489)
                     /locus_tag="VFU72_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5423377.1"
                     /translation="MITTTLEDGRPDANAVQVLELLRRAVSQLQPGIEWIGDIAPQLT
                     IQQLRVMGILYIEGPTRVSTLAQRLNVSTPTVTGILDRLVQRDMTVRVDDPSDRRVVL
                     NVLTDEGRHVIERFHPLEPARLADAVARLSEHDQQALIDGLTVLLSVAGRQ"
     gene            1813..2469
                     /locus_tag="VFU72_07560"
     CDS             1813..2469
                     /locus_tag="VFU72_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5423378.1"
                     /translation="MAERPQLSAEPRTIRGKQVSRLRRQGIAPGVVYGPPLDAPRPIS
                     IETHALERVFYAHGPSALVDLSVDGETYPVFIRSLQREPIRHAVLSVEFYAPQMNQPV
                     VSTVPVIATGALASTVDGILTHTRESVDVRGLPDQIPSHFEVDVAQLDEVDAAILVSD
                     LTLPPGIELLTPLEEILVKVAAPSRVPEPAPAAEEALAEETGDMPQAATEDIPPVEEP
                     "
     gene            complement(2724..3143)
                     /locus_tag="VFU72_07565"
     CDS             complement(2724..3143)
                     /locus_tag="VFU72_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423379.1"
                     /translation="MAPRRTATVVRGGGLRRWVRSPLADALVDVAPDLLRLTGRAVRP
                     HPLAGAAPGLPPSGANGLTVSEIEIDVASPLLRRVVVRSTNAWSLAPEVALGKRRRGG
                     LLRLGAVSVAGLTLLGIVMGRRAPRSLPSRVPRQDPF"
BASE COUNT          624 a         1291 c         1218 g          529 t
ORIGIN      
        1 cagccgcgtc ttggttgtgg tcgggcggga ggggtcgatc agcacgccgt cggtattcac
       61 gccccgcgcc cgcagcagcg tgcgcagctc gcgtccctcg gcgtcgtcgc cgaccacgcc
      121 cacctgccag acctcgccac cgagcgcgcg caccgccagc gccggggccg tcccaccgcc
      181 cggccgctcc tcgcggcgcg tctcctccag caccatgatc ggcgcctcgc gcgagacgcg
      241 cgccggatga ccaaagatgt agcagtcgag atagaggtcg cccaccacca gcagcgtgcg
      301 gccgcggatg gccgccagcg cggcccgcag ctccgtgttg acactcaccg gaagcggatc
      361 caggggatga gcaacgccag cgcggcaaag agcgcgtccg gcagcaggcg caactggaag
      421 aagctgctga tcgccggctg cgtggcgtgg tgcagcagca tgtccaaggt gaagaagggg
      481 ctgcgctcgg ccatgaccac gcgcgccacg accatcccga caatcaccag caggatgccc
      541 gccagttgca ggtccgcgcc ccgcttctcc tgcgccgccc aggcgatgcc ctcggccact
      601 ccgaagccga gcagcagcgc catgtagaag cgccagtcgg ggatgacgcc ccagaggatg
      661 ccgaccgcga gcgccaccac cagacccacg acgatcgcct tcaccatcgt cgccgggccg
      721 gtactgtagg tcggcaggcc gcgcaccgcg gcgcaggtcg ggcaacgaaa gccgaccggc
      781 gtctcgaccg cgcatcgggg gcagatgggc gtgccgcact tgctgcagcg caggcgggtg
      841 cggacattgg gatgataggt acagcgctgc tcttcgatat cggacagcac caccaggggt
      901 ctccatcagt ctagcttggg ggcacgagcc gggcgagaca gccaccctgc cagtgccgcg
      961 gcggacctga ggtccgccgc caacactatg ccatagacag cgtggttgtt gctcggtttg
     1021 gctgtgccta ctggcgcccg gcgaccgaga ggagaacggt cagaccgtcg atcagcgcct
     1081 gctggtcgtg ctcgctgagg cgcgcgacgg cgtcggcgag ccgggccggc tccagcgggt
     1141 gaaagcgctc gatgacgtgg cgtccctcgt cggtcagcac gttgagcacc acgcgccggt
     1201 cggacggatc atcgacgcgc acggtcatgt cgcgctgcac cagccggtcc aagatgccgg
     1261 tcacggtggg tgtcgagaca ttgagacgct gcgccagcgt cgaaaccctg gtcggcccct
     1321 cgatatagag gatacccatg acgcgcaact gctggatggt cagctgtgga gcgatatccc
     1381 cgatccactc gatgccgggc tgcaactgcg aaaccgcgcg ccgcagcagc tccaacacct
     1441 ggaccgcatt cgcatctggc cgtccgtcct cgagcgtggt tgtaatcatg gacgcatcgg
     1501 atcctttcac gttgccggga actttccccc ggtcgtttac tcccgatctg ccccaagctc
     1561 cccacctgga accattcgct catagatcgc ctcggggcaa tcgcttccga cgcgccgagc
     1621 aagcggcgaa gatgaacaaa tggtcccctc atgcctgtac atgcataata ccacgtcttc
     1681 cgcgcgtgcg ggcagcgttc ctgcgaattc aacgcccccc tggcgagcaa gccctacccg
     1741 gcgtgccgta tgcttcagcg cgcgtggggt cagcgccttc gagcccgatc acgacagtca
     1801 ggaaggaaga acatggcaga gcgaccacaa ttatcggccg agccgcgtac gattcgcggc
     1861 aagcaggtca gccgcctgcg gcggcaaggg atcgcgccgg gcgtggttta cggaccgccg
     1921 ctcgacgcgc cgcggccgat cagcatcgag acgcacgcgc tggagcgggt cttctacgcc
     1981 cacggcccca gcgccctggt cgacctgagc gtcgacggcg agacctaccc ggtcttcatc
     2041 cgcagtctgc agcgcgagcc gatccggcat gcggtgctga gcgtcgagtt ctacgcgccc
     2101 cagatgaacc agccggtggt ctcgaccgtg ccggtcatcg ccaccggcgc cctggcctcc
     2161 acggtcgacg gcatcctgac gcacacgcgt gagtcggtgg atgtgcgcgg cctgccggac
     2221 cagattccgt cgcacttcga ggtcgatgtg gcgcaactcg acgaggtgga cgccgcgatc
     2281 ctggtctccg acctgacgct gccgcccggc atcgaactgc tgacacccct ggaggagatc
     2341 ctcgtcaagg tcgccgcacc gtcgcgcgtg ccggagccgg cgccggcggc cgaggaagcc
     2401 ttggccgagg agacgggcga tatgccgcag gcagccaccg aggacatccc accagtcgag
     2461 gagccgtagt cctgtccccg ctgtgagtgg cccggcccat cgagggcgac gtgtggcctg
     2521 tgaggacgga tccgtcgact gcgctcagag cctgccctga gcctgccgaa gggatgaccg
     2581 ggtagaggat ggggcctttc ggacgacacg agccgtcttc gtccgcccct cactcaccct
     2641 gtcatcctga gcttgtcgaa ggatccgtcg tggtgcggca ctcaacattg tcggccggca
     2701 ggggtgagtg agcgcgggcg cggctagaac gggtcctgcc gcggaactcg cgacggcagc
     2761 gagcggggcg cacggcgccc catgacgatg cccagcagcg tcagcccggc cacgctcacc
     2821 gcacccagcc ggagcagccc gccgcgccgc cgcttgccca gcgcgacctc cggtgccagc
     2881 gaccaggcgt tggtcgagcg caccaccacc cgtcgcagca ggggcgaggc gacgtcgatc
     2941 tcgatctccg agacggtgag gccgttggcg cccgagggcg gcaggccggg cgccgcgccg
     3001 gccagcggat gcggccgcac cgcgcgcccc gtcaggcgca gcaggtcggg cgccacgtcg
     3061 accagcgcgt cggccagcgg cgagcgcacc cagcggcgca gcccaccacc acgaacgacc
     3121 gtcgcggttc gccgtggcgc cacctcggct cgcagtggca cggctggaag tttctcgccc
     3181 atatccccgc ccttcgccat tccatcagtt gacgctgctc gatcctaccc gcaagctcag
     3241 tccctcgcaa gttcgcactc gccgagtgat tgacaagcgg gcagcgggcc gctaggatga
     3301 aagaacggtc cagcgggacc gtgtcggatt tgctcgcgat ggaagggcca ggctgatgca
     3361 gatggctccc aacatcatca tccacagcca gtgggactgg ccgccgagcc tcggcgagct
     3421 gcccgtcttg gctaatggtg ggatcagtct gcccaggagc ggggtgacct gagcgagcca
     3481 atcgcgcaag cgccatccga gccgcgggca gagacgagaa tctcgcccgc ggtttttgtt
     3541 tgtgccccaa actcgcgcgc cacctgggtg cgccggccgc ggacgggatc gtgtgaacga
     3601 ggggaaacag gacagaggat gacattcgac cagctgcgat caagccacac gctcagcctg
     3661 ct
//
LOCUS       DASUBI010000391         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_281684, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000391 DASUBI010000000
VERSION     DASUBI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281684"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..779
                     /locus_tag="VFU72_07570"
     CDS             57..779
                     /locus_tag="VFU72_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423380.1"
                     /translation="MIVARELTRVYELGGVLVRALDGVSLTIADGEFLAIMGPSGSGK
                     TTLMNLLGCLDTPSSGSYELDGEAVDGLDDDQLAGIRGRKIGFVFQSYNLLPRVPALD
                     QVALPLLYLGVPARERRELARVALEAVGLGDRLRHRPTELSGGQQQRVGIARALVKQP
                     SLILADEPTGNLDSHASAEILELFARLNQDTGITVALVTHEAEIAARTRRTITLRDGR
                     LVSDSGWETGAGGAARLDEVPA"
     gene            776..2020
                     /locus_tag="VFU72_07575"
     CDS             776..2020
                     /locus_tag="VFU72_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423381.1"
                     /translation="MTLADALDIAAAALTANKLRTLLTMLGVIIGVSAVIALVAVGRG
                     AQNQVEARIAGLGTNVLFVRPGSQTTGGVRAASGSAPTLTLDDAAALAQLDGVSGVAP
                     ESTIAAQVSFQGENVNTRVVGTTPDYQDVRNFYPAEGDFLTQQDVSASSSVAVLGATT
                     AQNLFGDLDPVGQTIHIGRGQIAIPFRVVGVMESKGGTGLGNQDDQIFVPISTLVRKL
                     QVARTAEGSYQVGQIDVEVASKSRIDPAVQALGDLLRTRHRVAEDDFTIQSQEDTLQA
                     LTQTTRTFTILLGSIAAISLVVGGIGIMNIMLVSVTERTREIGIRKALGARRRDILLQ
                     FLVESLAVSALGGLLGVAVGVGAARLVNGRQLGAQTLYTSVAASSVLLAVLVAAAIGI
                     FFGLYPALRASRLNPIEALRYE"
     gene            2058..2597
                     /locus_tag="VFU72_07580"
     CDS             2058..2597
                     /locus_tag="VFU72_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423382.1"
                     /translation="MRRAFTQLLLGVIVLGLALGAAFGAGTLYGRHSATAAAAPAAQQ
                     ALVAASGQATPEAAGATGRTGRFAAGLGTPVAGGFGQGLGGGGFAAAARAVSGTVASV
                     SGQQVTITTAAGASVTVDLDAATRVGTVQRGELAALKAGDTVIVTTTRAGQGAQGGQG
                     GATAETASVVLILPGGFQP"
     regulatory      2637..2711
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2769..3137
                     /gene="crcB"
                     /locus_tag="VFU72_07585"
     CDS             2769..3137
                     /gene="crcB"
                     /locus_tag="VFU72_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5423383.1"
                     /translation="MVYLWLALGGALGTISRYTVSGWVAARYPSFPWGTFTVNIAGAL
                     VIGFFLTLAEERFLIAPEARVFVATGFLGGFTTFSTFSWETMALLRDGAFAPALGNAA
                     GSLLTGLFAVYLGIVLARLV"
BASE COUNT          425 a         1126 c         1161 g          460 t
ORIGIN      
        1 ggtggcttcg gtggcggtgg cggcgccggg cggaccggaa ccggaggccg cacgccgtga
       61 tcgtggcccg cgagctgacc cgggtctacg agttgggggg cgtgctggtg cgggcgctcg
      121 acggcgtcag cctgacgatc gccgacggcg agttcctggc gatcatgggc ccctccggct
      181 cgggcaagac gacgttgatg aacctgctcg gctgcctcga cacgcccagc agcggcagct
      241 acgagctgga cggtgaggcg gttgacggac tcgacgacga ccaactcgcc ggcatccgcg
      301 ggcgcaagat cggcttcgtc ttccagagct acaacctcct gccacgcgtg ccggcgctcg
      361 atcaggtggc tttgccgctc ctctacctcg gcgtgccggc acgcgagcgg cgcgagctgg
      421 cgcgggtggc gctggaggcg gtcgggctgg gcgaccggct gcgccaccgg ccgaccgagc
      481 tctccggcgg ccagcagcag cgcgtcggca tcgcccgcgc cctggtcaag cagccgagcc
      541 tgatcctggc cgacgagccg accggcaacc tcgacagcca cgccagcgcc gagatcctgg
      601 agctcttcgc ccggctcaac caggacaccg gcatcaccgt ggcgctggtg acccacgaag
      661 ccgagatcgc cgcgcgcacc cggcgaacca taaccctgcg cgacggccgg ctggtctccg
      721 acagcggctg ggagacgggt gccgggggcg ccgcgcgcct ggacgaggtg ccggcgtgac
      781 gctggccgac gcgctcgata tcgccgccgc cgcgctgacc gccaacaagc tgcgcaccct
      841 gctgacgatg ctcggggtga tcatcggcgt cagcgccgtg atcgcgctgg tggcggtcgg
      901 ccggggcgcc cagaatcagg tcgaggcgcg catcgccggc ctcggcacca acgtgctctt
      961 cgtgcgcccc ggcagccaga ccacgggcgg ggtgcgcgcg gccagcggtt cggcgccgac
     1021 cctgacgctc gacgacgcgg cggcgctcgc gcagctcgac ggcgtcagcg gcgtggcgcc
     1081 ggagagcacc atcgccgcgc aggtctcctt tcagggtgag aacgtcaaca cgcgcgtggt
     1141 cggcaccacg ccggactacc aggacgtgcg caacttctac ccggccgagg gcgacttcct
     1201 gacgcagcaa gatgtgtcgg cctccagctc ggtggccgtg ctgggcgcga ccacggcgca
     1261 gaatctcttc ggcgacctcg acccggtcgg gcagacgatc cacatcggcc gcggccagat
     1321 cgccatcccc ttccgtgtgg tgggggtgat ggagtcgaag ggcggcaccg ggctcggcaa
     1381 ccaggacgac cagatcttcg tcccgatctc gacgctggtg cgcaagctgc aggtggcgcg
     1441 caccgccgaa ggctcctatc aggtcggcca gatcgacgtc gaggtggcca gcaagagccg
     1501 catcgacccg gccgtgcagg cgctgggtga cctgctgcgc acgcgccacc gcgtagccga
     1561 ggacgacttc accatccaga gccaggagga cacgctgcag gcgctgacgc agacgactcg
     1621 gaccttcacc atcctgctcg gcagcatcgc ggctatctcg ctggtcgtcg gcggcatcgg
     1681 gatcatgaac atcatgctcg tctccgtcac cgagcgcacc cgcgagatcg gcatccgcaa
     1741 ggccctgggc gcccggcgtc gcgacatcct gctgcagttc ctggtcgagt cgctggcggt
     1801 cagtgcgctg ggcgggttgc tcggggtggc cgtcggagtg ggcgcggcgc gcctggtcaa
     1861 tgggcgccag ctcggcgcgc agacgctcta cacctcggtg gccgccagct cggtgctact
     1921 ggcggtgctg gtcgcggcgg cgatcggcat cttcttcggt ctctatccgg cgttgcgcgc
     1981 ctcgcggctc aacccgatcg aggcgctgcg ctacgagtag ccggagcggg ctcgctccgg
     2041 tggagagtgg gacgacgatg cggagagcct tcacccaact gctgctcgga gtcatcgtgc
     2101 tcggcctggc gctcggtgcg gccttcggcg ccggcaccct ctacggccgc cacagcgcga
     2161 cggccgcggc ggcgccggcc gcccagcagg cgctggttgc cgcgtcgggt caggcgacac
     2221 ccgaagccgc cggggcgacc gggcgtaccg gccgcttcgc cgccgggttg ggcacccccg
     2281 tcgcgggcgg gttcggccag gggctcggcg gcgggggctt cgcggccgcg gcacgcgccg
     2341 tcagcggcac ggtcgcgagc gtcagcggcc aacaggtgac gatcaccacc gccgccggag
     2401 cgagcgtgac ggtcgacctc gacgcggcca cccgggtcgg cacggtgcag cgcggcgagc
     2461 tcgccgcgct gaaggcgggc gacacggtga tcgtgaccac cacgcgcgcc ggccagggag
     2521 cccaaggcgg ccagggcggc gcgacggcag agaccgcctc ggtcgtgctg atcctccccg
     2581 gcggcttcca gccgtagcgg cgcgccatac ctcgacaagc ctgctggagg gcgtatcata
     2641 cgccgggcgg tggggtccgc ccgggcgcgc gcgcccgaac cgccgcggag gccgatgact
     2701 cctgctcgtt cgagcaggag ttttcctgtg ccgcgcaccg ttgtgcctgg gaagggagcc
     2761 gcggcggcat ggtctatctc tggctggcgc tggggggcgc gctcggtacg atcagccgct
     2821 acaccgtctc cggctgggtg gccgcgcgct accctagctt cccctggggc accttcaccg
     2881 tcaacatcgc gggcgcgctg gtgatcggct tcttcctgac gctggccgag gagcgcttcc
     2941 tgatcgcgcc cgaggcgcgc gtcttcgtgg ccaccggctt tctgggtggc ttcaccacct
     3001 tctcgacctt cagctgggag accatggcgc tcctgcgcga cggcgccttc gccccggcgc
     3061 tgggcaacgc ggcaggttct ttgctgaccg ggctcttcgc ggtctacctg ggtatcgtgc
     3121 tggctcggct ggtgtaagga gacggtccca tggcgctcga aggcggtaaa gt
//
LOCUS       DASUBI010000392         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1143860, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000392 DASUBI010000000
VERSION     DASUBI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143860"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFU72_07590"
     CDS             complement(<1..308)
                     /locus_tag="VFU72_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423384.1"
                     /translation="MLGSSRVVGVLLTAAGVVLGLGIFAWLIVSSREAAGGKSGGAVL
                     GGVLALACIILPLLAGGILLMLRGQAEVKDYGEVQKERAVLNMVETRGQVKISDIALE
                     T"
     gene            complement(411..1544)
                     /locus_tag="VFU72_07595"
     CDS             complement(411..1544)
                     /locus_tag="VFU72_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5423385.1"
                     /translation="MKLFTLGDMLNITPALRALRVRYPAARIDVLATRVGATGLARSP
                     YVDDVIIFDKTLFDRVGGLASPRALLAGLRFALTLRRRRYDVLVLLHHLITTWGTLKY
                     TVLALWSGAPVRAGLDNGRGWFLTHRARDRGFGAVNERRYWLDVVATLGAVSDNEQPQ
                     FTVTDADRAAAQELLGPTSGPVIVIHPTNGPYAPKRQWAPERFAAVGDRLAREHGATI
                     VLVGVASEAARIAAVERAMATLALNLAGRTDLPVLAGILQRADLVIGNDSAVCHLAAA
                     LGTPTLAIFGPANDRAWAPYGAQPVVLPLNEQQQPCPPDSRVLTVRGADPHAPCLYTG
                     YGPGNPHGCPNCRCLDLVDTDRIAVLAAHLLARPAARVATAAN"
     gene            complement(1744..>2058)
                     /locus_tag="VFU72_07600"
     CDS             complement(1744..>2058)
                     /locus_tag="VFU72_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423386.1"
                     /translation="TGAEALVFPSLYEGFGLPAIEAMACGTPVLTSNTSSMPEVAGDA
                     ALLVDPLDTAAIAAGIERIATDQALRAMLRERGLAHAATFTWRRAAEQTLAVLEEVGN
                     EA"
BASE COUNT          338 a          675 c          741 g          304 t
ORIGIN      
        1 gtttccagcg cgatgtccga aatcttcacc tgaccgcgcg tctccaccat gttcagcacg
       61 gcgcgctctt tctgcacctc gccgtagtct ttgacctcgg cctggccacg cagcatgagc
      121 aagatgccgc cggcaagcaa gggcaggatg atgcaggcga gggcgagcac gccgcccagc
      181 accgcgccgc cgctcttgcc ccccgccgcc tcacgcgacg agacaatcaa ccaggcgaaa
      241 ataccgagac cgaggacgac acctgcggcg gtgagcagaa cgccgacgac gcgactgctg
      301 ccaagcacag acacctcctg agtgacgagt aatgaacgag tattggctgc tgtcgtctcg
      361 gttcttacga gggccgacgc cccatatacg tcaggtggcg cggcggcgtt tcagttggct
      421 gctgtggcga cgcgggcggc aggtcgcgcc agtaggtgag cggccaggac ggcgatgcgg
      481 tcggtgtcaa cgaggtcgag acagcggcag ttggggcaac cgtgcggatt gccgggacca
      541 tagccggtgt agagacaggg cgcgtgcggg tcggcgccgc gcacggtcag cacgcggcta
      601 tccggcgggc agggttgctg ctgttcgttc aggggcaaca cgactggttg cgcgccgtaa
      661 ggcgcccagg cgcggtcatt cgccgggccg aagatggcga gcgtcggcgt gccgagcgcg
      721 gcggcgaggt ggcagaccgc gctatcgttg ccgatgacga ggtcggcgcg ttgcaggatg
      781 ccggcgagca ctggcaggtc ggtgcggccg gcgaggttga gcgccagcgt cgccatcgcg
      841 cgctcgacgg cggcgatgcg cgccgcttca ctggcgacgc cgaccaggac aatcgtcgcg
      901 ccgtgctcgc gcgccagtcg gtccccgacc gccgcgaagc gttccggcgc ccactgccgt
      961 tttggcgcgt aggggccgtt cgtcgggtga ataacaatca ccgggccgct ggtgggaccg
     1021 aggagctctt gtgccgccgc ccggtcggcg tcggtgacgg tgaattgtgg ctgctcatta
     1081 tcgctgaccg cgccgagcgt ggcgacgacg tcgagccagt agcgccgctc gttgaccgcg
     1141 ccgaagcctc ggtcgcgcgc ccggtgggtc aggaaccagc cccggccatt gtcgaggccc
     1201 gcgcgcaccg gcgcgccgct ccacagtgcc agcacggtgt atttcagcgt gccccaggta
     1261 gtaatgaggt gatggagcag caccagcacg tcatagcgcc ggcggcgcag cgtgagcgcg
     1321 aagcgcagcc cggccagcag agcgcgcggc gaggccaggc cgccgacccg gtcgaagagc
     1381 gtcttgtcaa agatgatgac gtcgtcaacg taaggcgagc gcgccagccc ggtggcgcca
     1441 actctagtcg ccagcacgtc aatgcgcgcg gccggatagc gcactcgcag cgcccgcagc
     1501 gccggcgtaa tattcagcat atcgccgagc gtaaagagtt tcactaccag aatgcgctga
     1561 ggcgcggcag gcggcgacgg cgcgggcgct gggacgtagc gcgagcggag aatggcagcg
     1621 gttggccccc ccagcccccc aattctgggg ggagctacgc tggtcaaccc ccttagtttt
     1681 gcagaaaggc cccggcgtag ggagagatgc tccccccaga attgaggggc cgggggggcc
     1741 atcttacgct tcattcccta cttcctccag caccgccagt gtctgctcgg ccgcgcgccg
     1801 ccaggtgaag gtggcggcgt gggccaggcc gcgctcacgc agcattgcgc gcaacgcctg
     1861 gtcggtggcg atacgctcga tgccggcggc gatcgcggcg gtgtcgagtg ggtctaccag
     1921 cagcgccgcg tcgcccgcca cttcgggcat tgacgaggtg ttgctggtca ggacgggcgt
     1981 gccgcaggcc atcgcctcga tcgccggcag gccgaagccc tcgtagagcg acgggaagac
     2041 gagcgcctcg gctccagt
//
LOCUS       DASUBI010000393         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_953444, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000393 DASUBI010000000
VERSION     DASUBI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953444"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2351)
                     /locus_tag="VFU72_07605"
     CDS             complement(<1..>2351)
                     /locus_tag="VFU72_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5423387.1"
                     /translation="DYLGSIADPARRALVERLFATFEAETLPKLASLRASVIYNDAND
                     YNALVRAEEPGEPQAVGVIDFGDMLRTATVCELAIGCAYAMFGKPDPLAAAAHVVAGY
                     HDALLLSEDELAALYPLILARLCVSVTNSAYQQAIDPGNAYLQISDRPAWETLERLAD
                     VPPALALALFRDACGLPASPTSPAVVAWLKAHGETFTPLVAPELTAGGVMTLDLSIGS
                     RELGNFPDFAETEPFTRRVFARMAEAGVALSIGRYNEPRPIYTTDAYRIEGNDGPEWR
                     TLHVALDLFAVAGTPIFAPLEGVVHSFQDNAAPRDYGPTIILEHTVTNETTGAPLTFY
                     TLYGHLSRDSLDGLREGMAVARGTEIARIGEEAVNGGWPPHLHFQIISDLLGKRGDFP
                     GVARPSQRAVWLSLSPDPHLIARLPIAAAPEGEPPRAVEQLLDVRRRHLGGNLSVAYR
                     RPLKIVRGYRQYLYDEDGRAYLDAVNNVPHVGHNHPRVVAAGQRQMAVLNTNTRYLHD
                     ELARYAERLCATLPEPLRVCYFVCSGSEANELALRLARAHTRRKGLVVVDVAYHGNTT
                     TLVEISPYKFNGPGGSGPAPYIRVVPLPDVYRGLYRDDPAAGPRYAAHVAQAIAEARA
                     AGGDEIGAFIAESMLGCGGQIVLPEGYLAAAYEHVRAAGGVAIADEVQTGFGRAGSHF
                     WAFETQGVVPDIVTMGKPIGNGHPLAAVVTTPEIAASFDNGMEYFNTFGGKPVSCAIG
                     LAVLDVIEEEGLQAHAQRTGAHLLAGLRGLMEGHAIVGDVRGQ"
BASE COUNT          332 a          847 c          825 g          347 t
ORIGIN      
        1 ctggccgcgc acgtcgccga cgatcgcgtg gccctccatc agcccgcgca ggcccgccag
       61 cagatgggcg ccggtgcgct gcgcgtgcgc ctgtaggccc tcctcttcga tcacgtccag
      121 caccgccagg ccaatcgcgc acgagaccgg cttgccgccg aaggtgttga agtactccat
      181 gccgttgtcg aacgaggcgg cgatctccgg cgtcgtcacc accgcggcga gcgggtgccc
      241 gttgccgatc ggcttgccca tcgtcacgat atcgggcacc acgccctgcg tctcgaaggc
      301 ccagaagtgc gagccggcgc gtccgaagcc cgtctgcacc tcgtcggcga tggcgacgcc
      361 gcccgccgcc cgcacgtgct cgtatgccgc cgccagatag ccctccggca gcacaatctg
      421 cccgccgcag ccgagcatag attccgcgat gaaggcgccg atctcgtcgc cgcccgccgc
      481 ccgcgcctcg gcgatcgcct gcgccacgtg cgccgcgtac cgcgggccgg ccgccggatc
      541 gtcacggtag aggccgcggt agacgtcggg cagcgggacc acgcggatgt acggcgcggg
      601 gccgctgccg ccggggccgt tgaacttgta cgggctgatc tcgaccagcg tggtcgtgtt
      661 gccgtggtag gccacgtcca ccaccaccag ccccttgcgc cgcgtgtgcg cccgcgccag
      721 ccgcagcgcc agctcgttcg cctcgctgcc cgagcagacg aagtagcaga cgcgcagcgg
      781 ctccggcagg gtcgcgcaga gccgctctgc gtagcgcgcc agctcgtcgt gcaggtagcg
      841 cgtgttggtg ttgaggacgg ccatctggcg ctggccggcg gccaccacgc gcggatgatt
      901 gtgcccaacg tgcggcacat tgttgacggc gtccagatat gcccggccgt cctcgtcgta
      961 gaggtactgc cggtagccgc gcacgatctt cagcggccgg cgataggcga cgctcagatt
     1021 gccgccgaga tgccgccgcc ggacatccag gagctgctcg accgcgcgcg gcggctcgcc
     1081 ctccggcgcc gccgcgatgg gcaaccgcgc gatcagatgc gggtcggggc tgaggctgag
     1141 ccagaccgcg cgctggctgg gccgcgccac gccagggaag tcgccgcgct tgcccagcag
     1201 gtcgctgatg atctggaagt gcaggtgcgg cggccagcca ccattgaccg cctcctcacc
     1261 gatgcgcgcg atctccgtcc cgcgcgccac cgccatcccc tcgcgcagcc cgtcgagcga
     1321 atcgcggctg agatggccgt agagcgtgta gaaggtcagc ggcgcaccgg tggtctcatt
     1381 ggtgacggtg tgctccagga tgatcgtcgg gccgtagtca cgcggggcgg cgttgtcctg
     1441 gaagctatgg acgacgccct ccagcggcgc aaagattggc gtgccggcca cggcgaagag
     1501 gtccagcgcg acgtgcagcg tgcgccactc cggcccgtcg ttgccctcga tgcggtaggc
     1561 gtcggtcgtg tagatcggcc gcggctcgtt atagcggccg atgctgagcg cgacgccggc
     1621 ctccgccatc cgcgcaaaga cacgccgcgt gaagggctct gtctcggcga agtcggggaa
     1681 gttgcccagc tcgcggctgc cgatgctcag gtcgagcgtc atcacgccgc ccgccgtcag
     1741 ctccggcgcc accagcggcg tgaacgtctc gccgtgcgcc ttcagccagg cgaccacggc
     1801 cggcgaggtg gggctggccg gcagcccgca ggcatcgcgg aagagggcca gcgccagcgc
     1861 gggcggcacg tcggccagtc gctccagcgt ctcccaggcg ggccggtcgc tgatctggag
     1921 gtaggcgttg ccgggatcta tcgcctgctg ataggccgag tttgtcacgc tgacgcagag
     1981 tcgcgccagg atcagcggat agagcgcggc tagctcgtcc tcgctcagca gcagtgcgtc
     2041 gtggtagccg gcgacgacgt gcgccgcggc agccagcgga tcgggcttgc cgaacatggc
     2101 ataggcgcag ccgatcgcca gctcgcagac cgtcgccgtg cgcagcatgt cgccgaagtc
     2161 aatcacgccg accgcctgcg gctcgcccgg ctcctccgcc cgcaccagcg cgttgtagtc
     2221 gttggcgtcg ttgtagatca cgctcgcccg caggcttgcc agcttcggca gcgtctcggc
     2281 ctcgaatgtc gcgaacagcc gctcgaccag cgcccgccgc gcgggatcgg cgatggagcc
     2341 gaggtagtcg t
//
LOCUS       DASUBI010000394         8340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_492937, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000394 DASUBI010000000
VERSION     DASUBI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492937"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1351
                     /locus_tag="VFU72_07610"
     CDS             200..1351
                     /locus_tag="VFU72_07610"
                     /inference="COORDINATES: protein motif:HMM:NF040505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HEU5423388.1"
                     /translation="MITNPHQSRQRPDQTRLVDVVVIGGGQAGLAAGYYLRRSSLDFV
                     ILDAGFGPGGAWRETWDSLTLFSPARWSSLPGSVMPGGIERFPGRDQVVAYLTDYERR
                     YRLPVARPVLVERVLSGTDSLVVETDRGAWRARAVSSATGTWSNPIIPCYPGQERFLG
                     EQLHAAGYRTPLAYTGERVLVVGGGNSGAQIMAELSALAEGTWVTRRPPSFLPDDVDG
                     RVLFERATSRYLRGQAERPGDDALRPLGEIVMLPAVREARERGALEAVRPFTAFSQSG
                     VIWPNGREEPIDVVIWATGFRPALGHLASLDLFENDGRIAVAGTRSLRQPQLWLLGYG
                     NWTGWASATIAGVGRAARATVEEVAAVLGDEGKPDRQTSNVGVRGRGRV"
     gene            complement(1382..1978)
                     /locus_tag="VFU72_07615"
     CDS             complement(1382..1978)
                     /locus_tag="VFU72_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5423389.1"
                     /translation="MIRDPDRRRRTLRLKNYDYAEPAAYFVTIVTHDRACLFARLEAD
                     EMRLNQLGLVAAEVWLDLPTHYPHVELDEFVVMPNHLHGIITLLDQPDVSVDGTETHL
                     GELPGDGGRAGFKPAPTGGTVRRRQALPEIVRAFKTFSARRINLARGTRGTPVWQRGY
                     YEHIIRNDASLDRIRQYILGNPAAWSNDRENPFADVTG"
     gene            complement(2045..2116)
                     /locus_tag="VFU72_07620"
     tRNA            complement(2045..2116)
                     /locus_tag="VFU72_07620"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2082..2084),aa:Met,seq:cat)
     gene            complement(2449..4206)
                     /locus_tag="VFU72_07625"
     CDS             complement(2449..4206)
                     /locus_tag="VFU72_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEU5423390.1"
                     /translation="MGRIPRRRGVVAMAALLIVLLTQVNLIPAAGAAPPDGQGAAGGG
                     IEQVDKTPPGKDHARHPLLLKVRGRDRSNQPLVTSTTPSGYTPRQIRGYLGLSGDGSG
                     QTIALVVAYDHPAIVSDLNVFSQQFGLPLVCGTPGASAGSCVNFSKATPQGTPQVNSG
                     WALEAALDVEWAHAVAPQAALLLVEASSAWTSSLFSAIDYAAQQGASVISNSWGTNEY
                     WGETSFDRHCALSTAVCTFASGDGGNPGGYPAYSPYVVAVGGTTLSLDSSGGVLGETA
                     WSGSGGGVSAYESRPAFQGTVQSTAYRGIPDVSYDADPNTGFAVYDSVSYSGQSGWFQ
                     VGGTSAGAPQWSGILADADQLRASQGKPRLTGAASGANSALYQLSNTLYDVSSGSNGT
                     CGAACQASAGYDFVTGLGSPRAGIDAALAGTGGTPSPTLSHDVAVTAISAPGAVTQGQ
                     AATVSVTVANPGGYSESVSVALGSSPANAAGPLAPKTIALSPGQSSSVSFSWATTSAT
                     TAGSYTLTATAGISGDANSANNSRSTSIQVNAPAQTSGQMYVQSLTLSQQSSRFLSYA
                     LVGSVTIKSSAGPVANATV"
     gene            4434..6689
                     /locus_tag="VFU72_07630"
     CDS             4434..6689
                     /locus_tag="VFU72_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5423391.1"
                     /translation="MFEGFGARADLLEGLNEAQVRAVTAGDGPVLIVAGPGSGKTRVL
                     THRIAYLIDERGVSPWNILAVTFTNKAAREMRERLERLIGPEQARKLTVGTFHATCAR
                     ILRQHGQHAGIDPRFTIYDDADQIETVKQALKALNLDPKEFSPRGILSRISSAKSRFV
                     RPEQFAEVTESYFDEIVARVYPRYQETLRQNRALDFDDLLAETLRLFDEQPEIKQRYQ
                     EWYRYVLVDEYQDTNHVQYLLVRALAERHRNLCVVGDPDQSIYGWRQADIRNILDFKR
                     DYPEAQEIHLELNYRSTGVIVAAADRVIRENTQRIQRQLRTENAEGEPIVLRECFDDT
                     QEAQFVAGEIRRLSYTGRFHERQVAVMYRTNAQSRALEEAFIRGEIPYQLIGGTRFYE
                     RREVKDTMALLRLIANPRDAVSLQRLLQNTPLGKGIGAKTLTDLERWARLEGRSVFDG
                     LAALAGELPLAPPQISGRAARLLGEVYATLSELVEASRRLVVTELFDLAVAETGIAAQ
                     LQQGGDPEMLERWENVLQLRAVLSGYDELPTESALETFLEESALVADADTMEESGNQV
                     TLITLHAAKGLEFPVVFLVGVEEGILPHARSLDTEDELEEERRLFYVGLTRAQERLYI
                     THAFRRSTYGRSDLAIRSRFVDAIPDELIESTEGRSAPPASRSGATIASPRARAQAQL
                     GLAPTPMPASAAPAALPSYSVGDRVFHQHFGDGQVVAVTAASGDLEVTVNFVRHGTKR
                     LMASLANLLTE"
     gene            6738..>8340
                     /locus_tag="VFU72_07635"
     CDS             6738..>8340
                     /locus_tag="VFU72_07635"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU5423392.1"
                     /translation="MDSRQWVYRFRDGNAEMRDLLGGKGANLAEMTNADLPVPPGFTI
                     TTEACNTFLGGGHKLPEGLWDQALGALHGVEEQMGRRFADPDNPLLVSVRSGAKFSMP
                     GMMDTILNLGLNDQTVRGLAAQAEPRFAYDCYRRLIQMFGKVVLAVDSELFEDAIDRA
                     KRKLRVQHDHELPAQALQDLVGEFKEIVRRESGKDFPSDPHEQLRQAIVAVFDSWNNR
                     RAIDYRRANNIPDDLGTAVNVQTMVYGNMGPDCATGVCFTRNPSTGEPGLFGEYLVNA
                     QGEDVVAGVRTPQPVQAMADDPALRPAYQQLLEIAERLERHYRDMQDLEFTVQNGTLW
                     MLQTRTGKRTGRAAVEVAVDLAEEGVIDQREAVARVEPGHLDQLLHPMIDDTRKGPVI
                     ATGLPASPGAASGKVVFNADEAKMLGDAGEAVILVRIETSPEDFHGMLVSRAVLTARG
                     GMTSHAAVVARGMGKPAVVGCGALNINYERQQMAVDGKVVHEGDFITLDGATGEVMLG
                     EIPTIPPQMGGKVETLLGWADQLRRL"
BASE COUNT         1300 a         2935 c         2827 g         1278 t
ORIGIN      
        1 ccatcgtcat gagccttcct ggggcggccg cctgaagcgc atgccgtgtc gtcgtgggcg
       61 gccaccagcg ctcgcttcgc aatccgtggt ccctgcctgc tcgacgccgc atgcgacaat
      121 cttcagcgta cgcggcgaag cgggacagcg gtagctgtcg agccgcgcca cgctagagtt
      181 ggaacggatg tcgaacgcgg tgatcacgaa tccgcaccag tcgagacagc ggcccgacca
      241 gacgcgcttg gtcgacgtcg tcgtgatcgg cggggggcaa gcggggctgg cggccggcta
      301 ctatctacgg cgcagcagcc tcgacttcgt catcctcgac gccggctttg gtcccggcgg
      361 cgcctggcgc gagacctggg actcgctcac cctcttctcc cccgcgcgct ggagctcgct
      421 ccccggcagc gtcatgccgg gcggcatcga gcgcttcccc ggccgcgacc aggtcgtcgc
      481 ctacctcacc gattacgagc gccgctaccg cctgccggtc gcgcgcccgg tgctggtcga
      541 gcgcgtgctg tccggcacgg atagtctcgt ggtcgagacc gatcgaggcg cctggcgggc
      601 gcgggccgtg agcagcgcca ccggcacctg gagcaacccg atcatcccct gctaccccgg
      661 ccaggagcgc ttcctgggcg agcaactgca cgccgccggc tatcgcacac cgttggcgta
      721 cacgggtgag cgcgtcctcg tcgtgggcgg gggcaactcc ggcgcgcaga tcatggccga
      781 gctctcggcg ctggccgagg gcacctgggt cacgcgccgc ccgccctcct ttctgccgga
      841 cgacgtcgac ggccgcgtcc tcttcgagcg cgccaccagc cgctacctgc gcggccaggc
      901 cgagcgcccc ggcgacgacg cgctgcgccc cctgggcgag atcgtcatgc tgccggcggt
      961 gcgcgaggcg cgcgagcgcg gcgccctgga ggccgtgcgc cccttcaccg ccttcagtca
     1021 gagcggcgtc atctggccca acggccgcga ggagccgatc gacgtcgtca tctgggccac
     1081 cggcttccgc ccggcgctgg ggcacctggc ctcgctcgat ctcttcgaga atgatggccg
     1141 aatcgcggtc gctggcacgc gctcattacg tcaaccacaa ctctggctgc tcggctacgg
     1201 caactggacg ggctgggcct cggccaccat cgccggcgtc ggccgcgccg cccgcgccac
     1261 ggtggaggag gtcgcggcgg tgctgggtga tgaagggaaa ccagacagac agacgagcaa
     1321 cgtcggcgta cgtggacgag ggcgcgtttg aaacccgccc ctacagggga tcgacgacac
     1381 cctatccggt gacatcggcg aacgggttct cgcgatcgtt agaccaagcg gccggattgc
     1441 caaggatgta ctggcgaatg cggtcgagcg atgcatcgtt gcgaatgatg tgctcatagt
     1501 agccgcgctg ccacacgggc gtaccccgcg tcccgcgtgc gagattgatt cggcgggcag
     1561 aaaacgtctt gaaggcgcgc acgatctcag gaagcgcctg ccgtcgccgc accgttccac
     1621 ctgtaggggc gggtttgaaa cccgcccggc cgccatcgcc cggcagttcc ccaagatgcg
     1681 tctcggtgcc atcgacggac acgtccggct ggtcgagaag ggtgatgatc ccatgcaggt
     1741 gattcggcat caccacgaac tcgtcgagtt cgacatgcgg ataatgcgtg ggcaaatcga
     1801 gccaaacttc tgcagcgacc aaacccaatt gattcaaccg catctcgtcc gcttccagtc
     1861 tagcgaagag gcacgcccga tcgtgcgtca cgatcgtgac gaaataggcc gccggctcgg
     1921 cgtaatcgta atttttcagg cggagggtac gtcgacggcg atccgggtcg cggatcatgg
     1981 cacgttcacc ccctcacgca aaacgacgcg ggccaaagcg ccgcgtcgtc attctaccgt
     2041 ctagtgccgg tgggcggatt tgaaccaccg accaagggct tatgagtccc ctgctctacc
     2101 cctgagctac accggctcgt gagcggtaga aagtataccg ctcctagcta tgcgggtcaa
     2161 acgcactcgc acggcctcgc acggcgacac ggcgccagac gctaacaatc catccggctc
     2221 cacgcgatga tgccaggcgt tcccctggca ctagccctag cgcaccgtca ccgtggcgct
     2281 cgattgctgg ttcagggaca actggtacgt gtagcccgtg gccgcggcgc tctggacttg
     2341 cagcgtgtag ctgccggtga tgtgcaccgt gcggctgaag gcggcgacgc cctgcgcgtc
     2401 ggtcgtcgcc gtgaaggtct ggctggtgta cgggcccgtc agccagagct acacggtggc
     2461 gttggccacc ggcccggcgc tgctcttgat ggtcacgctc ccgaccagcg cgtatgagag
     2521 gaagcggctg gactgctggc tcagcgtcag gctctgcaca tacatctgcc cgctggtctg
     2581 cgccggagcg ttgacctgaa tcgacgtcga gcggctgttg ttggcgctgt tggcgtcgcc
     2641 gctgatgccg gccgtggcgg tcagcgtgta gctccccgcc gtggtcgcgc tggtcgtcgc
     2701 ccacgagaac gacacgctcg acgactggcc ggggctgagc gcgatcgtct tgggggccag
     2761 cgggccggcc gcgttggccg ggctgctgcc cagcgccacg ctcaccgact cgctgtagcc
     2821 gccggggttg gccaccgtca cgctgaccgt ggccgcctgg ccctgtgtca ccgcgccggg
     2881 cgcgctgatc gccgtgaccg ccacgtcgtg ggagagcgtg ggcgaggggg tcccgcccgt
     2941 gccggccagc gcggcgtcga tgccggcacg cgggctgccc aggccggtca cgaagtcgta
     3001 gcccgcactc gcctggcagg cggcgccaca ggtcccattc gagccgctgc tcacgtcgta
     3061 gagcgtgttg ctcagttggt agagcgcgct gttggcgccg gaggcggcgc cggtcaggcg
     3121 cggcttgccc tggctggcgc gcaactgatc ggcatccgcc aggataccgg accactgggg
     3181 cgcgccggcg ctggtgccgc ctacctggaa ccagccgctc tggccactgt aggagacgga
     3241 gtcgtagacg gcgaagccgg tgttcgggtc ggcgtcgtag ctgacgtcgg ggatgccacg
     3301 ataagcggtg ctctgcaccg tgccctggaa cgctggccgg ctctcgtagg cgctcacccc
     3361 gccgcccgag ccgctccagg cggtctcgcc gagcacgccg ccgctgctgt ccaggctaag
     3421 cgtggtgccg ccgaccgcga ccacgtaggg gctgtaggcc gggtagccac cggggttacc
     3481 accgtcgccg ctggcgaagg tgcagaccgc cgtgctgagc gcgcagtgcc ggtcaaagct
     3541 ggtctcgccc cagtattcgt tcgtgcccca gctattgctg attacgctgg cgccctgctg
     3601 cgcggcgtag tcgatggccg aaaacaagct gctggtccag gcagacgacg cctccaccag
     3661 cagcagcgcg gcctgcggcg cgacggcgtg cgcccactcg acgtcgagcg cggcctccag
     3721 cgcccagccg ctgttgacct gcggcgtgcc ctgcggcgtc gccttgctga agttgacgca
     3781 gctcccggcg ctggcgccgg gggtgccaca gaccagcggc agcccgaact gctggctgaa
     3841 gacgttgagg tcgctgacga tcgccgggtg gtcgtaggcc accacgagcg cgatcgtctg
     3901 gccgctgccg tcgccggaga gaccgaggta gccgcggatc tgccgcggcg tgtagccgga
     3961 gggcgtggtg ctggtcacca gcggctggtt ggaccggtcg cgcccgcgca ccttgaggag
     4021 cagcgggtgc cgggcgtggt ccttgcccgg gggtgtcttg tcgacctgct cgatcccgcc
     4081 gccggctgcg ccctgcccgt ccggcggggc cgctccggcg gctggaatca gattcacctg
     4141 ggtcagcagc acgatcagca gcgccgccat ggcgaccacg ccacggcgcc gtgggattct
     4201 ccccacgctt cccttccctc tttctagacc cgtgccgagc ccgcggcctg ttcaccccga
     4261 ccgcgagcac gcccgattcg tagcgtgggc caatgatact cgcccctggt acccgatcac
     4321 cctgcgccgc tggtcccaat cgcgccgagt catttcgatg cccgcccagt cagagggagc
     4381 gccgggtggg tggtacaatt atccgtagca tgtacgaacg ggaggggcag gtcgtgttcg
     4441 aagggtttgg cgcgcgggcg gacctgctgg agggcctcaa cgaggcgcag gtccgggcgg
     4501 tgaccgccgg cgacggcccc gtgctgatcg tggccgggcc gggtagcggc aagacgcgcg
     4561 tgctcaccca ccgcatcgcc tatctgatcg acgagcgcgg cgtctcgccc tggaacatcc
     4621 tggccgtcac cttcaccaac aaggccgccc gcgagatgcg cgagcgtctg gagcggctga
     4681 tcggccccga gcaggcgcgc aagctgaccg tgggcacctt tcacgccacc tgcgcacgca
     4741 tcctgcgcca gcacggccag cacgccggca tcgacccgcg cttcaccatc tacgacgatg
     4801 ccgaccagat cgaaacggtc aagcaggcgc tcaaggcgct caatctcgat cccaaggagt
     4861 tctcgccgcg cggcatcctc tcgcgcatct cctcggccaa gagccgcttc gtccgccccg
     4921 agcagttcgc ggaggtcacc gagagctact tcgacgagat cgtggcccgc gtctacccgc
     4981 gctaccagga gacgctgcgc cagaaccgcg cgctcgactt cgacgacctg ctggccgaga
     5041 cgctgcgcct cttcgacgag cagcccgaga tcaaacagcg ctatcaggag tggtaccgct
     5101 acgtcctggt cgacgagtac caggacacca accatgtgca atacctgctg gtgcgcgccc
     5161 tggccgagcg gcaccgcaac ctctgcgtgg tcggcgatcc cgaccagtcg atctacggct
     5221 ggcgccaggc cgacatccgc aacatcctcg atttcaagcg cgactaccct gaggcgcagg
     5281 agatccacct ggagctgaac taccgctcga ccggcgtcat cgtggccgcc gccgaccggg
     5341 tcatccgcga gaacacccag cgcatccagc ggcagttgcg caccgagaac gccgagggcg
     5401 agccgatcgt gctgcgcgag tgcttcgacg acacccagga ggcccagttc gtggccggcg
     5461 agatccgccg cctctcctac accggccgct tccacgagcg ccaggtggcg gtgatgtacc
     5521 gcaccaacgc ccagagccgg gcgctggagg aggccttcat ccgcggcgag atcccctacc
     5581 agctcatcgg cggcacccgc ttctacgagc ggcgcgaggt caaggacacc atggccctcc
     5641 tgcgcctgat cgccaacccg cgcgacgccg tcagcctgca gcgcctgctg cagaacacgc
     5701 cgctgggcaa gggcatcggc gccaagacgc tgaccgacct ggagcgctgg gcgcgcctgg
     5761 agggccgctc ggtcttcgac gggctagcgg ccctggccgg cgagttgcca ttggcgccgc
     5821 cccagatcag cgggcgggcg gccaggctgc tgggtgaggt ctatgccacg ctctccgagc
     5881 tggtcgaggc cagccggcgg ctggtcgtca ccgagctgtt cgatctggcc gtggccgaga
     5941 cgggcatcgc tgcgcaactg cagcagggcg gcgatccgga gatgctggag cgctgggaga
     6001 acgtgctgca gttgcgcgcg gtgctgtctg gctacgacga gctgcccacc gagagcgcgc
     6061 tggagacctt cctggaagag tccgcgctgg tggccgacgc cgacaccatg gaggagagcg
     6121 gcaatcaggt gacgctgatc acgctgcacg ccgccaaggg gctggagttc ccggtggtct
     6181 tcctggtcgg cgtcgaggag ggcatcctgc cccacgcccg ctcgcttgac accgaggacg
     6241 agctggagga ggagcgccgc ctcttctacg tcgggctgac ccgcgcccag gagcgcctct
     6301 acatcaccca cgccttccgt cgctcgacct acggccgcag cgacctggcg atccgctcgc
     6361 gcttcgtgga tgcgatcccc gacgagctca tcgagtccac cgaggggcgc tcggcgccac
     6421 ccgcttcgcg gtctggcgcg acaattgcga gtccccgtgc tcgcgctcag gctcagctcg
     6481 ggctggcgcc cacgcccatg ccggcctcgg ccgcgcccgc cgctcttccc agctacagcg
     6541 tcggcgatcg cgtctttcac cagcatttcg gcgatgggca agtggtcgcc gtcacggcgg
     6601 cgagcggcga tctggaagtg accgtcaact ttgtgcgcca cggcaccaag cggctgatgg
     6661 cgagtctggc gaatctgctg acggagtaag atcgggcaac ggggcccggt cgcggcctac
     6721 gacgaggagg cagtcagatg gactcccgcc aatgggtcta tcgatttcgc gacggcaatg
     6781 ccgagatgcg tgacctgctg ggtggcaagg gcgccaacct ggccgagatg accaacgccg
     6841 acctgccggt tccccccggc ttcaccatca ccaccgaggc ctgcaacacc tttctgggcg
     6901 gcgggcacaa gctccccgaa ggcctctggg accaggcgct cggcgcgctg cacggggtcg
     6961 aggagcagat gggccggcgc ttcgccgacc cggacaaccc gctgctggtc tcggtccgct
     7021 ccggcgccaa attctcgatg ccgggcatga tggacactat cctcaacctc ggactgaacg
     7081 accagacggt gcgcgggctg gcggcgcagg ccgagccgcg cttcgcctac gactgctatc
     7141 gccgcctgat ccagatgttc ggcaaggtgg tgctcgccgt cgattccgag ctcttcgagg
     7201 acgccatcga ccgcgccaag cgtaagctgc gcgtgcagca cgaccacgag ctgccagccc
     7261 aggcgctgca ggatctggtc ggggagttca aggagatcgt gcgccgcgag agtggcaagg
     7321 atttccccag cgacccgcac gagcaactgc gccaggcgat cgtggccgtc ttcgactcct
     7381 ggaacaaccg ccgcgccatc gactaccgac gcgccaacaa catccccgac gatctcggca
     7441 ccgcggtcaa cgtgcagacc atggtctacg gcaacatggg gcccgactgc gccaccggcg
     7501 tctgcttcac ccgcaacccg agcaccggcg aaccgggact cttcggcgag tacctggtca
     7561 acgcccaggg cgaggacgtg gtggccggcg tgcgcacgcc gcagccggtg caggccatgg
     7621 ccgacgaccc ggcgctgcgc ccggcctacc agcaattgct cgagatcgcc gagcggctgg
     7681 agcggcacta ccgtgacatg caggacctcg agttcaccgt gcagaacggc accctctgga
     7741 tgctccagac gcgcaccggc aagcgcaccg gccgcgccgc ggtcgaggtc gcggtcgatc
     7801 tggccgagga gggcgtgatc gaccagcgtg aggcggtcgc gcgcgtcgag cccggccacc
     7861 tcgatcagct cctgcacccg atgatcgacg acacgcgcaa gggtccggtg atcgccaccg
     7921 gcctgccggc cagcccgggc gcggccagcg gcaaggtcgt cttcaacgcc gacgaggcca
     7981 agatgctcgg cgatgccggc gaagcggtca tcctagtgcg catcgagacc tcgcccgagg
     8041 acttccacgg catgctggtc tcccgcgcgg tgctgaccgc gcgcggcggc atgacctcgc
     8101 acgctgccgt ggtcgcacgt ggcatgggta agccggcggt ggtcggctgt ggcgcgctga
     8161 acatcaatta cgagcgccag cagatggccg tcgatggcaa ggtggtccac gagggcgact
     8221 tcatcacgct cgacggcgcc accggcgagg tgatgctggg cgagatcccg accatcccgc
     8281 cgcagatggg cggcaaggtc gagacgctgc tcggctgggc cgaccagcta cgccgcctgg
//
LOCUS       DASUBI010000395         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_965089, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000395 DASUBI010000000
VERSION     DASUBI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965089"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1296
                     /locus_tag="VFU72_07640"
     CDS             487..1296
                     /locus_tag="VFU72_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5423393.1"
                     /translation="MGRASWDRVESRGFGVAGEYPRGTIDVAARAAEQAGFRSFWLSQ
                     PPERDSLLTLSEVARLTDTIELGIGAIPFTLRPAQSVVDAVVRLELPLRRLRLGVGSG
                     TEPGALDRIRQGVREIKQQLDVHVAVAPLGPKMCRIAGEVADSVLLNWLVPEFARTSI
                     EWVREGAASVSRDTPLVCTYVRCAIGQASLPRLRQESDRYGSFPHYAAHFRRQGVEPI
                     AATIHAESRDELQSRLAEYEDVLDQVIVRAVTPHDEPDEVLALIEHARPRR"
     gene            complement(1327..2985)
                     /locus_tag="VFU72_07645"
     CDS             complement(1327..2985)
                     /locus_tag="VFU72_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5423394.1"
                     /translation="MSQRQFDPVSLEIMWSRLIGITDDMWTTVLRTAVSTIIGAAQDF
                     GCEILDASGSSLAHSNRSMPSFNVIMPYVVRNVIERFPTDDMAPGDVFITNDPWVCAG
                     HLDDIALVTPIFHREHVVAFASSIGHATSVGGTLSFRTARDLYEEGLFIPLTRLYAGG
                     EPNETAFRFIGENVRTPDMVLTDIEAQITANELGARRLREFLDEYQLADLAALAEAVQ
                     AQSERATQQAIAALPDGTYESDVLADGQDHPIHLCCQVEIKGDQIAIDFEGSDPQQPR
                     GGINCTLSYTRAHTLYPMKCLLSPSVPANEGTYRPITVNAPEGSILNCRRPASVRSRP
                     RTGWHLHALLFQALAPILPRGVQAGNGLMHAFNVYGQERDGSFYNAHFFAAGGRGASL
                     GRDGVGRNCFPSSARNVPVEQFEIRAPVVVHHRELRPDSTGTGTWRGAFGQRMVFGTL
                     PGHGAPVSFFVDPDRMCFPPLGLAGGENGPLAEIRVNDRSLTLEDLGEGQITLATADD
                     RLEMWVPGGGGYGPPEERDPALVEADIRAGLITPARVPVPASGE"
     gene            complement(2998..3516)
                     /locus_tag="VFU72_07650"
     CDS             complement(2998..3516)
                     /locus_tag="VFU72_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5423395.1"
                     /translation="MADQTELPNTVQALRDLVDASWRRLQPAFDAPEAVVTRVGADGW
                     SIKDHLAHITAWEGALLATLAGQPWLDSMGLGDLAQAPSDIDAINALILARRQSQPAA
                     SVRAEAQAIHQRVEAALAALSDTDLARPNSYFYPSAPEPLRDEPVAGWIAINTFQHYD
                     DHQLAIRALLGA"
     gene            complement(3600..>4548)
                     /locus_tag="VFU72_07655"
     CDS             complement(3600..>4548)
                     /locus_tag="VFU72_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423396.1"
                     /translation="EAAVHEPSSSFPAPAFESVVRPVEPGRLTLLRAALEELAEQDPL
                     ISLRQRNAEGEIAVRLYGEIQKEVLQETLAHDYGVAVTFGPTQTICVERPLGSGEHAE
                     LMGADGNPFYATLGFRVEPGAPGSGRRYRHEPGSLPPSFYRAVEETVRETLEQGLFGW
                     RVTDCDVTLTQVGYSSVVSTAGDFRKLTPLALMRALAQAGTEVCEPIDELELECPEDC
                     FGAVCGLLLIKRASIGQVARGGDGQRLVCVIPSAELRGLEQQLPGLTRGEAGWDSRFA
                     GYVPASGEPPRRRRIDPDPLNRAHYLAEVARAKSSSVSA"
BASE COUNT          704 a         1552 c         1575 g          717 t
ORIGIN      
        1 atcgtctcgg tcatggcgcg acggattgca tcgtctctcg acatctgcct ccccttattt
       61 ctccaagcca ggcccggcga tcgagaacca gcacgtcagc tgattggcgc ctcatctcct
      121 tcctgacccc gtgggatcga tcgtcgcgcg gccgatgatc tagcgccgga tagtcaaaca
      181 cacggaccca aatgtgatca agcactctgg acgttcgcgc ttctggtgag ggatgagagc
      241 accgttcgcc gcctcgggca agcgtgaatc cactcccgcc cgtggtgtga aggcctcacc
      301 acgcagaata gcacgcgcga gcgcctggtg gagcaggagt agaacctccc ggtctagtct
      361 ccggaaagca gccacgccga ccggcggctt gacggccctg gacccgccag ccgatcatgc
      421 cgccggacga ggttcgcatc aacaacgcgg gcgtcatcgg ttcggcaccg gcacatggcg
      481 cgcaccatgg ggagggctag ctgggatagg gttgagtcac gagggtttgg cgtagcgggc
      541 gagtatccca gggggacgat cgacgtcgcg gcgcgggccg ccgagcaggc cggctttcgc
      601 tcgttctggc tgagccagcc gcccgagcgt gactcgctgc tgaccctctc tgaggtcgca
      661 cgcctgaccg acacgatcga gcttggcatc ggcgcgatcc ccttcacgct ccgcccggcc
      721 cagagcgtgg tcgacgcggt cgtgcggctc gagctgcccc tgcgccggct gcggctcggc
      781 gtcgggagcg gcacggagcc gggtgcgctc gatcggattc gccagggagt gcgtgagatc
      841 aaacagcagc tcgacgtgca cgtggcggtc gcgccgctgg ggccgaagat gtgtcgcatt
      901 gccggcgaag tcgcggacag cgtgctgctc aactggctcg ttcccgaatt cgcacgaaca
      961 tcgatcgagt gggttcgcga gggcgccgcg tcagtcagtc gcgacacgcc gctcgtctgt
     1021 acctacgtgc gctgcgcgat tggtcaggcc tcgctgccac ggctgcggca ggagagcgat
     1081 cgctacggct cgttcccgca ctatgcggcg catttccggc ggcagggggt tgagccgatc
     1141 gcggcgacga tccacgccga gagtcgggat gagctgcagt cgcgcctggc cgagtacgaa
     1201 gacgtgctcg atcaggtcat cgtacgggcg gtgacgccgc acgacgagcc cgatgaggtg
     1261 ctcgcgctga tcgagcacgc tcgcccgcgc cgctgacctg gcaatcgcca gactctcttg
     1321 ggcgcgctac tcgccgctgg ccggcaccgg cacgcgagcg ggcgtgatga ggccagcccg
     1381 gatgtcggcc tcgacgagcg cggggtcgcg ctcctccgga ggcccgtagc cgccaccacc
     1441 gggaacccac atctccagcc ggtcatccgc ggtcgccaac gtgatctggc cctcgcccag
     1501 gtcttccagc gtcaggctgc ggtcgttgac ccggatctcg gcgagcggac cattctcgcc
     1561 tcccgccagg ccgagcggcg ggaagcacat gcggtccgga tcgacaaaga aggacacggg
     1621 agcgccgtgg cccggcaatg tgccgaacac catgcgctga ccgaacgcgc cgcgccaggt
     1681 gcccgtgccg gtcgagtcgg gccgcagctc gcgatgatgc acgaccaccg gcgcgcggat
     1741 ctcgaactgc tcgaccggga cattgcgcgc gctgctgggg aagcagttac ggccgacccc
     1801 gtcccggccc aggctcgctc cgcgcccgcc cgccgcgaag aagtgcgcgt tgtagaagct
     1861 gccgtcgcgc tcctggccgt agacgttgaa ggcgtgcatc aacccgttgc cggcctgaac
     1921 ccctcgcggg aggatcggcg ccagcgcctg gaagagcagg gcgtgcaggt gccagccggt
     1981 gcgtggccgc gagcggaccg acgcgggccg gcggcagttc agaatcgagc cctcgggcgc
     2041 gttcacggtg atcggtcgat aggtcccctc gttcgcgggc acactgggcg aaaggaggca
     2101 cttcatcgga tagagggtat gcgcgcgggt gtagctgagc gtgcagttga tgccgccgcg
     2161 cggctgctgc gggtccgagc cttcgaagtc gatcgcgatc tggtcgccct tgatctcaac
     2221 ctggcagcag aggtgaatcg ggtgatcctg gccatcggcg agcacgtcgc tctcataggt
     2281 gccgtccggc agcgcggcga tcgcctgctg cgtggcccgc tccgactgcg cctgcaccgc
     2341 ctccgccagc gcggccaggt ctgccagctg gtactcgtcg aggaactcgc gcaggcggcg
     2401 cgcgcccagc tcgttggcgg tgatctgcgc ctcgatatcg gtcagcacca tgtcgggcgt
     2461 gcgcacgttc tcgccgatga accggaaagc ggtctcgttc ggctcgccgc cggcatagag
     2521 ccgtgtcagg gggatgaaga gcccttcctc gtaaaggtca cgcgcggtgc ggaaggacaa
     2581 tgttccgccg acgctggtgg cgtgacctat ggaggacgcg aaggcgacca cgtgctcgcg
     2641 gtggaagatc ggggtgacca gcgcgatatc gtcgagatgg ccggcgcaga cccaggggtc
     2701 gttggtgatg aagacgtcgc cgggcgccat gtcgtcagtc gggaagcgct cgatgacgtt
     2761 gcgcaccacg taaggcatga tcacgttgaa agatggcatc gagcggttgg agtgggccag
     2821 ggagctgccg gatgcgtcca ggatctcgca gccgaagtcc tgggccgcgc cgatgatggt
     2881 cgagaccgcc gtacgcagca cggtggtcca catgtcgtcg gtgatgccga tcaagcggct
     2941 ccacatgatc tccaggctga ccggatcgaa ttgccgctgg ctcatgctgc cccctcctta
     3001 cgctccgagc agcgcgcgga tcgcgagctg atggtcgtcg tagtgctgga aggtgttgat
     3061 cgcgatccag ccggcgaccg gctcgtcgcg cagcggctcc ggcgcggatg gatagaaata
     3121 tgagttgggg cgcgccagat cagtgtccga gagcgcggcc agggccgctt cgacgcgctg
     3181 gtggattgcc tgggcctcgg cccgcacgct cgccgccggt tgagactgcc ggcgcgccag
     3241 gatgagcgcg ttgatggcgt cgatgtcgct cggcgcctgc gccagatcgc cgagccccat
     3301 gctgtcgagc cagggctggc ccgccagcgt ggcgaggagc gccccttccc aggcggtgat
     3361 gtgtgccagg tgatccttga tcgaccagcc gtcggcgccg accctggtga cgaccgcctc
     3421 cggcgcgtcg aaggcgggct gcagccgccg ccaggacgcg tcgaccaggt cgcgcaacgc
     3481 ctgcacggtg ttcggcagct cggtttggtc ggccatcgcg cgacgctcct cccagcgttg
     3541 tcgctccagc cggttcgccc gctcgcgccg gctccccgcg atccctatcg taccagccat
     3601 tatgccgaca ctgaactgct cttggctcgc gccacctcgg ccaggtagtg ggcgcggttg
     3661 agcgggtcgg ggtcgatgcg ccggcgccgg ggcggctcgc cgctggcggg tacgtagccg
     3721 gcgaagcgcg aatcccaacc ggcctcgccg cgcgtcaggc ccggcaactg ctgttcaagg
     3781 ccgcgtagct ccgcgctggg gatgacgcag accaaacgct gcccgtcacc gccgcgcgcc
     3841 acctggccga tcgaggcgcg cttgatgagc aagagcccgc agaccgcgcc gaaacagtcc
     3901 tccggacact ccagctccag ttcgtcgatc ggctcgcaca cctcggtgcc ggcttgagcc
     3961 aatgcccgca tcagcgccaa cggggtcagc ttgcgaaagt cgccggccgt gctcaccacc
     4021 gagctgtagc ccacctgggt gagcgtcacg tcgcagtcgg tcacgcgcca accgaatagc
     4081 ccctgctcca acgtctcacg cacggtctcc tcgacggcac ggtagaacga cggcggcagc
     4141 gagcctggct cgtgccgata gcgtcgcccg ctccccggcg cacccggctc gacccgaaag
     4201 cccagcgtgg cgtagaaggg gttgccgtcc gcgcccatca gctcggcgtg ctcgcctgag
     4261 cccagggggc gctcgacgca gatggtctgg gtcggcccga aggtcaccgc cacgccgtag
     4321 tcgtgcgcca gcgtctcctg cagcacctcc ttttgaatct cgccgtagag gcggaccgcg
     4381 atctcgccct ccgcgttgcg ctggcgcagc gagatcagcg ggtcctgctc cgccagctcc
     4441 tccagcgcgg cacgcaagag cgtgagccgg cccggctcaa caggacgcac gacgctctcg
     4501 aacgcgggcg ccgggaacga cgagctgggc tcgtgtaccg cggcctca
//
LOCUS       DASUBI010000396         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_564843, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000396 DASUBI010000000
VERSION     DASUBI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564843"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..1260)
                     /locus_tag="VFU72_07660"
     CDS             complement(394..1260)
                     /locus_tag="VFU72_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5423397.1"
                     /translation="MKTTDRATQPAVVLTGAGSGIGAACAVALSQAGHRVFAGVRWEP
                     DAGAWQGGRAELITPLMLDVTDAGSIARAAAEVEEIVGEAGLAGLVNCAGIVLGGPLE
                     FLAPVELRRQLDVNVVGQVAVTQAFLPLIRRDHGRIVNVGSVSGRNALPFLGPYAASK
                     FALRALNDALRVELRPWGIPVILIEAGSIATGIWAKGAAEAHRQFAGFPPAARGLYGK
                     QLDTLTTRVVELGERGLPAQRVAETVTLALTVRHPQAHYAVAPAGRRWLTRLLGVLPT
                     GLRDRLVLRALR"
     gene            complement(1278..2021)
                     /locus_tag="VFU72_07665"
     CDS             complement(1278..2021)
                     /locus_tag="VFU72_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5423398.1"
                     /translation="MIEVDIALVADIHGNRWALEAVLEDIERLGAAQIVDLGDSLLGP
                     LDPAGTADLLMARAIPSLRGNCDRIILDEAGATDSPTKRLADRVLTANQRAWLAALPA
                     TRVLDGGILLCHGTPQADDHFLLERASIDGPTLRPPTEIADDLNDVRQEVIGCAHSHI
                     PRLVALPSGQVILNPGSIGVPAYFEELPWPHKMETGSPHARYATLSDGPHGWHVSFHA
                     VPYDWERAARSAAEVMGPAYADWIRTGRA"
     gene            complement(2227..2508)
                     /locus_tag="VFU72_07670"
     CDS             complement(2227..2508)
                     /locus_tag="VFU72_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423399.1"
                     /translation="MDAWGFTMLVAGALVIGLAAQVLVRTRFGYEWIATAIGAFIGGY
                     LPAEYALGGLGQWGTQVSGLYIYPALIGAILVGLVVEVVIYFVEQPAPA"
     gene            2738..>3817
                     /locus_tag="VFU72_07675"
     CDS             2738..>3817
                     /locus_tag="VFU72_07675"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5423400.1"
                     /translation="MGAWRPALMGRHGVVASAHPLASAAGLRMLEEGGNAVDAAVAVA
                     AALNVAEPYMSGLGGDGYMLITLPGASQPLVLDYNGAVPREADPAHYTQAEQQTGPKA
                     PLVPGAPAGWLTALESYGTMSPAEVFAPAIDYAEHGAPVTFKNTEFIAGAAERIRAFP
                     LTAETFLPGGQPPRPGSLLRQPRLARTYRVLAEEGAHAFYHGEIGDEIVRAVRAAGGV
                     LSTRDLAEFAIDWVEPLSIDFRGYRVFAPPPPGAAIEYLELLRIVEPENLQALGHNSA
                     AYLHLLIEADKLAGADRITYTMSGVDPNTLLSDSYVDQQRQRIARERASLGQSDHYAA
                     ELAEAVRAGNPAAASRDHTTHFGVAD"
BASE COUNT          588 a         1365 c         1332 g          532 t
ORIGIN      
        1 cgagcaggtg atccacaacc tgaccgccga cctcgacctg acgctggcgc tctgcggccg
       61 ccgcgacgta gccgagatcg acggatcgct ggtaagccgg gtggcgaacg aatagcatag
      121 gacgcagggc aagacggatc cttcgacttc gcgcaggatg acagggtaag agcggggctg
      181 acgaggagga tgggccttgg gcaacgacat tcctcgtcgt cagcccgcca ttacccctga
      241 cgctcctatg tcaaggttag gcggcctgcg ccagcggcgg ggcctgacat cccgaccgaa
      301 gtggagggat ccgggccccc ggcggcaccc agcatcgtcg aagggcagtt cccctctccc
      361 gcgcatcggg agaggggtca ggggtgaggg ccctcaccgc agcgcgcgca gcaccaggcg
      421 gtcgcgcagg ccggtaggga gcacgcccag caggcgggtc agccagcggc ggccagccgg
      481 tgccacggcg tagtgcgcct gggggtgccg gacggtgagc gccagcgtga cggtctcggc
      541 cacgcgctgg gccggcaggc cgcgctcccc cagctcgacc acgcgcgtgg tcagcgtatc
      601 gagttgcttg ccgtagagcc ctcgcgccgc cgggggaaag ccagcgaact gccggtgggc
      661 ctccgccgcg cccttggccc agatgccggt cgcgatcgat cccgcctcga tcaggatcac
      721 cgggatgccc cagggccgca gctcgacccg caacgcgtcg ttgagcgcgc gcagggcgaa
      781 cttggaggcg gcgtaggggc cgaggaaggg cagggcgttc cggccggaga ccgagccgac
      841 gttgacgatc cggccgtgat cgcgccggat cagcggtagg aaggcctgcg tcacggctac
      901 ctgcccaacg acgttgacat cgagctggcg gcgcagctcc accggcgcca ggaactccag
      961 cggcccgccc agcacgatcc cggcgcagtt gaccagcccg gccaggccgg cctctcccac
     1021 tatctcctcg acctcggccg ccgctcgtgc gatcgacccc gcatcggtca cgtcgagcat
     1081 gaggggagtg atcagctcgg ctcgcccacc ctgccaggcg cccgcgtccg gctcccaccg
     1141 caccccggcg aagacccggt ggcccgcctg gctcagcgcg accgcgcagg ccgccccgat
     1201 gccgctcccc gccccggtga gcacgaccgc tggctgggtg gcgcgatcag tggttttcac
     1261 ccgtacctca gtgccgctca cgcccgcccg gtccgaatcc agtccgcgta ggccggcccc
     1321 atgacttcgg ctgctgagcg cgccgctcgc tcccagtcat acgggaccgc gtggaagctg
     1381 acatgccagc cgtgcggtcc atcgctgagc gtggcatagc gcgcgtgcgg ggagccggtc
     1441 tccatcttgt gcggccacgg cagctcttcg aaataggccg gaaccccgat gctgccgggg
     1501 ttgaggatca cctgcccgct gggcagcgcc acgagccgcg ggatgtggct gtgagcgcag
     1561 ccgatcacct cctggcgcac gtcgttcagg tcgtccgcga tctcagttgg cggccggagt
     1621 gtcggaccgt caatcgaggc gcgctccagc aggaagtggt cgtcggcctg gggcgtgccg
     1681 tggcagagga gaatcccgcc gtcgagcacg cgcgtcgccg gcaaggcggc cagccaggcg
     1741 cgctggttcg cggttaggac acggtcggcc agccgtttcg tcggggagtc agtcgccccg
     1801 gcctcatcga ggatgatgcg gtcacagttg ccgcgcaggc tggggatagc gcgggccatc
     1861 agaagatcgg cggtgccggc tgggtcgagg ggaccgagca ggctgtctcc gagatcgacg
     1921 atctgagcgg cgccgagccg ttcgatgtct tccagcaccg cttccaacgc ccagcggttg
     1981 ccgtgtatgt cggcaacgag cgcgatatcc acctcgatca cctcttcctc gctcgtggaa
     2041 ggacgcggcg ccgcgcatta tcgcgctctt tcgtatccgc tccaagtgcc aaacaccggt
     2101 ctctcgccgc cgagcgctcg ggggcgatcg tcaaaggcga gcgtgacacc ggtccacagg
     2161 cgtgtgcggg gaggatgagc ggagggctac ctggcgcggc ttgggtggcc gcgccaggct
     2221 cgggggtcag gcgggagccg gttgctcgac gaagtagatg accacttcga cgaccagacc
     2281 caccagaatc gcgccgatca gcgccggata gatgtacagg ccgctgacct gcgtgcccca
     2341 ctgaccgagg ccaccgagcg catattcagc cggcagatag ccgccgatga acgcgccgat
     2401 cgccgtggcg atccactcgt agccgaagcg ggtccggacc aggacttgcg ccgcgagacc
     2461 gatgacgagc gcgccggcca ccagcatcgt gaaaccccag gcatccatta cgagcctccc
     2521 ttctctggcg agcgcctcgt cgcgctcgtg gctcgatgga catctgtcta cactgtaggg
     2581 ggtccagcat agcggcgccg tgaaagtgcg gcgccggtcg tgacaacatc gtgtcaaacc
     2641 gcactgatgg aggcaacggc gcggcccttt ccagatcgat ctccaccgtg taccgtgccg
     2701 gcgacggtgg ggatgcacgc gagaaggagg gaacgcgatg ggagcctggc gtccggcgct
     2761 gatgggccgg catggcgtgg tggcctcggc gcacccgctc gcctcggcgg cggggctgcg
     2821 catgctggag gagggcggca acgccgtcga cgccgcggtg gccgtggcgg cggcgctcaa
     2881 cgtggccgag ccctatatgt cggggctggg cggcgacggc tacatgctga tcacgcttcc
     2941 cggcgccagc cagccgctgg tgctcgacta caacggcgcc gtgccgcgcg aggccgaccc
     3001 ggcccactac acccaggccg agcagcagac cgggcccaaa gcgccgctgg tgccgggcgc
     3061 gccggccggc tggctgaccg cgctggagag ctacggcacc atgagcccgg ccgaggtctt
     3121 cgccccggcc atcgactacg ccgagcacgg cgcgccggtc accttcaaga acacggagtt
     3181 catcgcgggc gccgccgagc gcatccgcgc cttcccgctg accgccgaga ccttcctgcc
     3241 cggcggccag ccgccgcgcc ccggctcgct gctgcgccag cctcgcctcg cgcgcaccta
     3301 ccgcgtgctg gccgaggagg gcgcccacgc cttctaccac ggcgagatcg gcgacgagat
     3361 cgtgcgcgcg gtgcgcgcgg ccggtggcgt gctcagcacg cgggatctgg ccgagttcgc
     3421 catcgattgg gtcgagccgc tgtcgatcga cttccgcggc taccgggtct tcgcgccccc
     3481 gcccccgggc gcggccatcg agtacctgga gctcctgcgc atcgtcgagc cggagaatct
     3541 ccaggcgctg ggccacaact cggccgccta cctccacctg ctgatcgagg ccgacaagct
     3601 cgccggggcc gaccggatca cctacaccat gagcggtgtc gatccgaaca ccctgctttc
     3661 cgatagttat gtcgatcaac agcggcagcg catcgcgcgc gagcgcgcct cgctcggcca
     3721 gagtgaccac tacgccgccg agctggccga ggcggtgcgc gccggcaacc cggccgccgc
     3781 ctcgcgcgac cacaccaccc acttcggcgt ggccgac
//
LOCUS       DASUBI010000397         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1073859, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000397 DASUBI010000000
VERSION     DASUBI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073859"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1053)
                     /locus_tag="VFU72_07680"
     CDS             complement(70..1053)
                     /locus_tag="VFU72_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5423401.1"
                     /translation="MSTDMRLDEQAVGEVRASAERIREAVERVIVGKRAVIDRLLVAL
                     LAEGHVLIEDVPGIGKTKLARSLSQALGGSFHRVQFTPDLLPSDVTGSLVYDQGKGEF
                     VFHPGPVFANVVLADEINRAGPRTQAALLEAMEERTVTVERATHPLPRPFLVIATQNP
                     VELEGTFPLPEAQLDRFFLSTAVGYPEAEDEREMLRRFRVGDPLAEVVPVARPEDLAA
                     MIAAVKRVFVGPAVEDYLLDLVRRTRAHEEVELGASPRAALALARAAQAHAAMAGRGF
                     VIPDDVRELAMPVLAHRIIPAARVELRGRTKADLLEEIVAGVPVPVEVGEA"
     gene            complement(1368..>2255)
                     /locus_tag="VFU72_07685"
     CDS             complement(1368..>2255)
                     /locus_tag="VFU72_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5423402.1"
                     /translation="VQQLAALLVERNYRALPVVDPAGKLVGLVTNGDLIERGSLGLRA
                     ELLHLLDPQALGQLLAKLAESGRTAADVMTADVVTVTPGTSLADAAHLMALKRLKRLP
                     VVDAEGRLMGIVARVDVLRAATAGAGAQPVDAETEVPAASPGATVAEVMTTQAPTVAA
                     NAGLPEVIEAVVSTRLNRAVVVDEERRPLGVVTDAELLGRLDPAALPGLARLLMGKLP
                     FVRLSDAEREQLRFAQGTRAAELMLTPVLTVRGETPALEAASLMLQRRYKILPVVDAE
                     GRLIGLLDRADLLRAVARR"
BASE COUNT          315 a          853 c          792 g          295 t
ORIGIN      
        1 cagagccact ggcccagctt gtcgccgcgc ccgccgtgcg agatgccgcc gccctcgccg
       61 gtgtccatct caggcctcgc ccacttcgac cggcaccggc actccggcga cgatctcctc
      121 cagcaggtcg gccttggtgc gcccgcgcag ctcgacgcgc gccgccggga tgatgcggtg
      181 cgccagcacc ggcatggcca gctcgcgcac gtcatcgggg atgacaaagc cgcgtccggc
      241 catggcggcg tgcgcctggg ccgcccgcgc cagcgccagc gccgcgcgcg ggctggcccc
      301 cagctcgacc tcctcgtggg cgcgcgtgcg ccgcaccaga tcgagcaggt agtcctccac
      361 cgccgggccg acgaagacgc gcttgaccgc cgcgatcatg gccgccagat cctccggccg
      421 ggccaccggc accacctcgg ctagcgggtc gccgacgcgg aagcgccgca gcatctcgcg
      481 ctcgtcctcg gcctcggggt agccgacggc ggtgctcagg aagaagcggt cgagctgcgc
      541 ctccggcagc gggaaggtgc cttcgagctc gaccgggttc tgggtggcga tcaccaggaa
      601 ggggcgcggc agcgggtggg tggcgcgctc gaccgtcacc gtgcgctcct ccatcgcctc
      661 cagcagggcc gcctgggtgc gcggaccggc ccggttgatc tcgtcggcca gcaccacgtt
      721 ggcgaagacc ggtccggggt ggaagacgaa ctcgcccttg ccctggtcgt agaccagcga
      781 gccggtgacg tcggagggaa ggaggtcggg ggtgaattgc acgcggtgga aggagccgcc
      841 gagcgcctgc gagagcgagc gcgccagctt ggtcttgccg atgcccggca cgtcttcgat
      901 gagaacgtgc ccctcggcca gcagcgctac cagcaggcgg tcgatcacgg cgcgcttgcc
      961 gacgatgacg cgctcgaccg cctcgcggat gcgctccgcg ctggccctga cctccccgac
     1021 cgcctgctcg tcgagtcgca tgtccgtgct catctcgtcc tctttccagc gaacacagga
     1081 gcgcgtggcc cggcgctgcg cgccggcccg gctcgccagc gcagcgtacc acttcgctcg
     1141 ctccctcggg ggaggatgga gtgcggcgac ggcgaggcaa acaaaaagct cccacgcagc
     1201 gcccgatccg ggctggcgta gaagccatca gtcgcgtcga cggttcgacc tcggcgggac
     1261 gctctgcggg acgctctgcg agccgctcgc ctccgtcgcg gcgggcttca ccaccgcagc
     1321 cgcagtctac tcccgatcgt ccgccgtgcc aaccgatggc tcagcgctca gcgccgggcg
     1381 accgcgcgca ggagatcggc acggtcgagc aggccaatca ggcggccctc ggcgtcgacc
     1441 accggcagga tcttgtagcg gcgctggagc atcaggctcg cggcctccag cgccggggtc
     1501 tcgccgcgca cggtgagcac cggagtgagc atcagctcgg cggcacgcgt cccctgggcg
     1561 aagcgcaact gctcgcgctc ggcgtcggag agtcgcacga aggggagctt gcccatcaac
     1621 agccgggcca ggcccgggag cgcggcggga tcgagccgcc ccagcagttc agcgtcggtc
     1681 accaccccga gcgggcgccg ctcctcgtcg accaccaccg cccggttgag ccgcgtcgag
     1741 acgaccgcct cgatcacctc cggcagcccc gcgttggccg cgaccgtggg cgcctgggtg
     1801 gtcatcacct cggccacggt ggcgcctgga gaggcagccg gcacctctgt ctcagcgtca
     1861 accggctgcg cacccgcccc ggcggtcgcg gcgcgcagca cgtcgacgcg cgcgacgatg
     1921 cccatgagcc tgccctcggc atcgacgacc ggcagccgct tgaggcgctt cagcgccatc
     1981 agatgcgcgg catcggccag gctggtcccc ggcgtgaccg tcaccacgtc cgcggtcatc
     2041 acgtcggcgg cggtccggcc ggactcggcc agcttcgcca ggagctggcc gagcgcctgc
     2101 gggtcgagaa ggtgcaggag ctccgcccgc agacccaggc tgccgcgctc gatcagatcg
     2161 ccgttggtca ccaggccgac cagcttgccc gcagggtcga ccaccggcag cgcgcgatag
     2221 ttccgctcga ccagcagcgc ggccagttgc tggac
//
LOCUS       DASUBI010000398         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1077040, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000398 DASUBI010000000
VERSION     DASUBI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077040"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFU72_07690"
     CDS             <1..226
                     /locus_tag="VFU72_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423403.1"
                     /translation="EVHRRYFFHLRCPGEPPESWRHYETDPSEGTRQPIPFDFFWAKL
                     PDELPALIAGHDALIPRLLERLGARGDCNG"
     gene            219..1160
                     /locus_tag="VFU72_07695"
     CDS             219..1160
                     /locus_tag="VFU72_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5423404.1"
                     /translation="MADQIVRAAVVQAAPVAFDRAATLDKTARLAAEAAGRGAKLAVF
                     PEAFVSAYPRGLTFGATVGNRTPEGREEFRRYWESAVEAPGPAVDRLSAIARANALYL
                     VIGVIERDGGTLYCTVLFFAPDGAYLGKHRKLMPTAAERLVWGYGDGSTLPVFDTPLG
                     KLGAVICWENYMPLLRMTMYSKGVQLYCAPTADQRDSWIASMRHIACEGRCFVLSCNQ
                     FARRADYPADYAVDCDDPQAVLCRGGSVIVSPFGEFLAGPCFEGEAILTADLDLGEIA
                     RGKYDFDVVGHYARPDVFQLVVDERPKPPVVTRQEQG"
     gene            complement(1245..1640)
                     /locus_tag="VFU72_07700"
     CDS             complement(1245..1640)
                     /locus_tag="VFU72_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU5423405.1"
                     /translation="MAQQTRQLEVIQTDKAAAPIGPYSQAVRAGDFIFVAGEKGIDPS
                     TNQMVTGGIAAETRQTLENIKNILESAGSGMHRVVATTVYLVNIGDFNAMNAVYAEYF
                     TSNPPGRTTVGVQALPANAQVEITVTALA"
     gene            1757..4285
                     /locus_tag="VFU72_07705"
     CDS             1757..4285
                     /locus_tag="VFU72_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5423406.1"
                     /translation="MAVTGPEEQFETNPAPAERTSRLGRRSGASWPAPVLAPLIVGGA
                     ILAAAALAVFVAPADQLLTALIAAAILLAALRLDATLIAAGGLLGVIVYLAAGIALGA
                     AWPALVIGALVVAAAAGLAMLLARRPAPLPPAVPRPAARARLAEREGEPVRSSEARVR
                     QALGNLPLLVFYQDRQLRYTWARGPHAGFDPATLPGKTDQELFPSHEARALHELKRAV
                     LAGEPRRQDVWLTIAGERRCFDLALQPLLGPGDEVQGLSGVLSDITERKLAEGQERRR
                     ARRQSALAGLALAALADGDELGRRAVATVAAELGTGLVGLFAVQGAEALLTAGVGWAE
                     GLPGQARAPLAGTLLGQVLEAGEPLARGELLPGDLDRLPLLRDAGWRALLGAPVQTGE
                     QPAALVLAGSPTPREWSRQEAAFLTAVTHILASASRVPVPAHAALYDPLTHLPNRALL
                     LDHLARALARAARREALTGVVVLSLAAGAGEADDALIGAAVERLRRALRPSDLLARTG
                     PAEFALIVEDAGQAAAVVQIAERLVAVAEQAGPRGRAHAGVALGAAGRPAEELLERAG
                     QARDWALSPGAPPVALDDPSLGAASLDEQQRVVDLRRALAAGALELRYEPVLAADGAL
                     VALEVHIGWPAHVELDARALWTLAAQAELDDALMVWTLTWASRDLLGWPGWPTSTARL
                     IVPAAPRLLAEPPAVEQVAGLLAAAGLAPDRLLLAVDEGALGDDRVLERLGALRQLGT
                     GLLLDGFGLGRLAPARLAGLGAAVYRLDRSLTAEPLDASVVGGLARLSHGLGAQVAAL
                     EVDSDEGCRQLRAAGCDLVQGSAVAAPLTAAQLAAAPARFGPAS"
     gene            complement(4409..5080)
                     /locus_tag="VFU72_07710"
     CDS             complement(4409..5080)
                     /locus_tag="VFU72_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5423407.1"
                     /translation="MNTNLEPRVPLGTTRRKLLQILKQSDGLAVDQLAERLGITPMAV
                     RKHIAALEADGLLESTTVQGPVGRPAHVYFLTPQAEEHFPKQYDRLLTDLLADLACLD
                     GAGKVDLLLARRSERTQQFLAERLDAAASLGERVAALAEGMDELGYLASWEEVEPGVY
                     RIKQYNCAIDQVASCFPGACACEAEMFRRLLGAEVERSSHMLAGDRCCCYVVRAGVEG
                     EQHAG"
     gene            complement(5128..>5624)
                     /locus_tag="VFU72_07715"
     CDS             complement(5128..>5624)
                     /locus_tag="VFU72_07715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5423408.1"
                     /translation="RDDRMTAKPTAGASEEQMHIVPPPVLYLGALFLGLVGQRVRPVR
                     PLPRGLARPLGWLLIGGGLALGAWGVLTFRGAGESVDPTRPTQALVTGGPYRYSRNPL
                     YVGMTAIYLGITALANSLWPLLLLQPVQRLMNGRIIPAEEAYLARRLGDEYAEYRRRV
                     RRWL"
BASE COUNT          714 a         2031 c         2067 g          812 t
ORIGIN      
        1 tgaagtgcac cggcgctact tcttccacct gcgctgcccc ggcgagccgc ccgagtcctg
       61 gcggcattac gagacggatc ccagcgaggg aacgcggcag cccataccct tcgatttctt
      121 ctgggcaaag ctgcccgatg agttgccggc gctcatagcc ggccatgacg cgctgatccc
      181 gaggctgctg gagaggcttg gagcgagagg ggactgcaat ggctgaccag atcgtgcgcg
      241 cggcggtggt gcaggcggcg ccggtggcct tcgaccgcgc ggcgacgctg gataaaacgg
      301 cgcggctggc ggcggaggcg gccgggcgcg gcgcgaagtt agccgtcttc cccgaggcct
      361 tcgtctcggc ctacccgcgc ggcctgacct tcggcgccac cgtcggcaac cgcacgccgg
      421 aggggcgcga ggagttccgc cgctactggg agtcggcggt cgaggcgccg gggccggcgg
      481 tggatcgctt gagcgcgatc gcgcgcgcga acgcgctcta cctggtgatc ggggtgatcg
      541 agcgcgacgg cggcacgctc tactgcaccg tcctcttctt cgcgcccgat ggcgcctacc
      601 tcggcaagca tcgcaagctg atgccgaccg ccgccgagcg gctggtctgg ggctacggcg
      661 acggctcgac cctaccggtc ttcgacacgc cgctgggcaa gctaggcgcg gtcatctgct
      721 gggagaacta catgccgctc ttgcgcatga cgatgtacag caagggcgtc cagctctact
      781 gcgccccgac ggccgaccag cgcgactcct ggatcgccag catgcgccac atcgcctgcg
      841 aggggcgctg cttcgtgctc agctgcaacc agttcgcgcg ccgcgccgac tacccggccg
      901 actacgccgt cgactgcgac gatccgcagg cggtcctctg ccgcggcggc tcggtcatcg
      961 tctcgccctt cggcgagttc ctggccggcc cctgcttcga gggcgaggcg atcctgaccg
     1021 ccgacctgga cctgggcgag atcgcgcgcg gcaagtacga cttcgacgtc gtcgggcact
     1081 atgcccgccc cgacgtcttc cagctggtgg tcgacgagcg ccccaagccg ccggtggtca
     1141 cgcgccagga gcagggttag acactggccg tcgcatagca tcgctgccgt tccacgacgg
     1201 atccgtggcg cacacgccac ccaacgtgtg cgccagacag cgccctacgc cagcgccgtc
     1261 accgtgatct cgacctgggc gttggccggc agcgcctgca cgccgaccgt ggtgcggccc
     1321 ggcgggttgc tggtgaagta ctcggcgtag accgcgttca tcgcgttgaa gtcgccgatg
     1381 ttgaccagat agacggtcgt cgccaccacg cgatgcatcc ccgagccggc cgactccagg
     1441 atgttcttga tgttctccag cgtctgacgt gtctcggccg cgatgccgcc ggtgaccatc
     1501 tggttggtgc tgggatcgat ccccttctcg ccggctacga agatgaagtc gccggctcgc
     1561 accgcctgcg aataggggcc gatcggtgcg gcggccttgt cggtctggat cacctcgagt
     1621 tgtcgcgttt gctgggccat gcgccctcct tccgcgctga tcatagcgct cccgtcagag
     1681 atataggaca actaggggac ggcttcgccg gtaccggccc gcgatactta tcgtggtaga
     1741 aaggtctgga gcgagcgtgg ccgtgaccgg tccggaggag cagtttgaga ccaacccggc
     1801 gccggccgag cgcacgagcc ggctagggcg gcgctcgggc gcttcctggc ctgccccggt
     1861 cctggccccg ctgatcgtgg gcggcgccat cctggccgcc gccgcgctgg ccgtcttcgt
     1921 cgccccagcg gaccagctcc tgaccgccct catcgccgcc gccatcctgc tcgccgcgct
     1981 gcgcctcgac gcgacgctga tcgcggccgg cggcctgctc ggtgtgatcg tctatctcgc
     2041 cgctggcatc gccctgggcg ccgcctggcc cgccctggtg attggcgcgc tggtggtggc
     2101 cgccgcggct gggctggcga tgctgctggc ccgtcgcccc gcgccgctgc ccccagcggt
     2161 cccacgcccg gccgcacgcg cgcggctcgc cgagcgggag ggcgagccgg tgcgctcgag
     2221 cgaggcgcgg gtgcgccagg cgctcggcaa cctgccgctc ctcgtcttct accaggaccg
     2281 ccagctgcgc tacacctggg cgcgcgggcc gcacgccggc ttcgaccccg ccacgctccc
     2341 cggcaagacc gaccaggagc tgtttccgtc gcacgaggcg cgggcgctgc acgagctgaa
     2401 gcgcgccgtg ctggcgggcg agccccggcg ccaggatgtc tggctgacga tcgctggcga
     2461 gcgtcgctgc ttcgatctgg cgctccagcc gctgctcggg ccgggtgatg aggtgcaggg
     2521 actgagcggc gtgctgagcg atatcaccga gcgcaagctg gccgaggggc aggagcgccg
     2581 gcgagcgcgg cggcagagcg cgctggccgg tctggccctg gcggcgctgg ccgacgggga
     2641 cgagctgggg cggcgggccg tcgccaccgt cgccgccgag ctcgggaccg ggctggtggg
     2701 gctcttcgcc gtacaggggg ctgaggcgct gctgacggcg ggcgttggct gggcggaggg
     2761 gctgcccggc caggcgcgcg cgccgctggc cggtacgctg ctcggccagg tgctggaggc
     2821 cggcgaaccc ctggcccgcg gcgaactgct cccgggcgac ctcgatcgct tgccgctgtt
     2881 gcgcgacgcg ggctggcgcg cgctgctcgg ggcgccggtc cagaccggcg agcaaccagc
     2941 cgcgctcgtg ctggcgggca gccccacgcc gcgtgagtgg tcgcggcagg aggccgcctt
     3001 cctgacagcg gtcacgcaca tcctcgcgag cgcgagccgt gtgccggtcc cggcccacgc
     3061 ggcgctctac gacccgctga cccatcttcc caaccgcgcg ctcctgctcg atcacctggc
     3121 gcgcgccctg gcacgcgcgg cacgccgcga ggcgttgacc ggagtggtcg tcctctcact
     3181 ggcggcgggc gcgggcgaag cggacgacgc tctgatcggg gcggccgtcg aacgcctgcg
     3241 gcgcgcgctg cggccgagcg acttgctggc gcgtacgggg cccgccgagt tcgccttgat
     3301 agtcgaggac gccgggcagg ccgcggcggt ggtgcagatc gccgagcgcc tggtcgcggt
     3361 ggccgagcag gcgggcccac gagggagagc acacgccggg gtggcgctgg gcgccgccgg
     3421 ccgcccagcc gaggagctgc tggagcgcgc cgggcaggcc cgggattggg cgctctcgcc
     3481 cggtgcccca ccggtcgcgc tcgacgaccc cagcctgggt gcggccagcc tggacgagca
     3541 gcagcgcgtc gtcgatctgc gccgcgcgct cgccgccggg gcgctggagc tgcgctacga
     3601 gccggtcctc gccgccgacg gcgcgctggt cgcgctggag gtgcatatcg gctggccggc
     3661 gcacgtcgag ctggatgcgc gagcgctgtg gacgcttgcc gcacaggcgg agctggacga
     3721 cgccctcatg gtctggacgc tgacctgggc cagccgggat ctgctcggct ggccgggatg
     3781 gcccacgtcc acggcgcggc tgatcgtgcc cgcagccccg cgcctgctcg ccgagccgcc
     3841 ggccgtggag caggtggccg ggctgctggc ggctgccggg ctggcgcccg accggctgct
     3901 gctggcggtc gacgagggcg cgctgggaga cgaccgcgtg ctggagcggc tcggcgcgct
     3961 gcggcaactg gggacagggc tgctgctgga cggcttcggg ctggggcggc tggccccggc
     4021 acgcctggcg gggctgggag ccgcggtcta ccggctcgac cgctcgctga cggcggagcc
     4081 gctcgatgcg agcgtcgtcg gcgggctggc gcggctgtcg cacgggctcg gcgcccaggt
     4141 ggcggcgctc gaggtcgact cggacgaggg ctgccggcag ttgcgcgcag cgggctgcga
     4201 cctcgtccag ggcagcgccg tggccgcgcc gctcaccgcc gcgcagctcg ccgccgcgcc
     4261 cgcgcgcttc ggtcccgcgt catagagctt cgttcccgcc acgatccgga cggtgggaac
     4321 aacggagaga tccttcggca acacacgaca gcaaccctgc gcggtagagg aggcccttca
     4381 cgcgtcggtt catcttgcgc tgggaacgtc agccggcgtg ctgctcgccc tccacgcccg
     4441 cccgtacgac gtagcagcag cagcggtcgc ccgccagcat gtggctactg cgctcgacct
     4501 cggcgccgag caagcggcgg aacatctcgg cctcgcaggc gcaggcgccg gggaagcaac
     4561 tggcgacctg gtcgatggcg cagttgtact gcttgatgcg gtagacgccc ggctcgacct
     4621 cttcccagct cgccaggtag cccagctcgt ccatcccctc ggcgagcgcc gcgacccgct
     4681 cgcccaggct ggcggccgcg tccagccgct cggccaggaa ctgctgggtg cgctcggagc
     4741 ggcgcgccag cagcaggtcg actttgcccg cgccgtcgag gcacgccagg tcggccagga
     4801 gatcggtcag cagccggtcg tactgcttgg ggaagtgctc ctcggcctga ggagtcagga
     4861 agtagacatg cgccggacgc ccgaccggcc cctgcacggt ggtcgactcc agcaggccgt
     4921 cggcctccag cgcggcgatg tgcttgcgca ccgccatggg cgtgatgccg agccgctcgg
     4981 ccaactgatc gaccgccagg ccgtcgctct gcttgagaat ctggagtagc ttgcgccggg
     5041 tcgtgccgag cggcacccgc ggctcaaggt tggtgttcac gcaacctccc gccgctatcc
     5101 taacataacg ggctacgccg cgagccgtta gagccagcgc cgcacccggc gccggtactc
     5161 cgcgtactcg tcgccgaggc gccgggccag gtacgcctcc tcggccggga tgatccgccc
     5221 gttcatcagc cgctgcaccg gctgcagcag caggagcggc cagaggctgt tggccagcgc
     5281 ggtgatgccg agatagatgg ccgtcatgcc gacatagagc gggttgcgcg agtagcggta
     5341 cggtccgccg gtcaccagcg cctgggtcgg ccgagtcgga tcgactgact caccggcacc
     5401 ccggaaggtc agcacgcccc aggcgccgag tgccagcccg cccccgatca ggagccagcc
     5461 gagcggacgc gccagcccac gcggcagcgg ccgcaccggc cggacccgct gccctaccag
     5521 cccgaggaag agcgctccca ggtagagcac gggcggcggc acgatgtgca tctgttcctc
     5581 ggaggcaccg gccgtcggtt tggcagtcat ccgatcgtcc cttt
//
LOCUS       DASUBI010000399         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_988507, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000399 DASUBI010000000
VERSION     DASUBI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988507"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU72_07720"
     CDS             <1..295
                     /locus_tag="VFU72_07720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5423409.1"
                     /translation="SALSRALDELGVAHTRLSFLSSVWSYEPDTRTCHYVDRLESFFR
                     VEATGDPRARPPEGYRWVPAEEAVARLMPGAHARALDDLRASSRVLPGPLLLC"
     gene            392..2821
                     /locus_tag="VFU72_07725"
     CDS             392..2821
                     /locus_tag="VFU72_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5423410.1"
                     /translation="MAAHATRSERTEEPDIRSASPERLAALDAIQRRILWLATNIIHH
                     ANHLRPNPDKTKVGGHQASSASIVTILTALYFQYLRAGDRVSIKPHASPAFHAVQYLL
                     GNLDRAYLPTLRAYKGLQAYPSRTKDPEPVDFSTGSVGLGAVAPVFAALAHQYSLAHF
                     GAVSSGRFVAVLGDAELDEGNVWEAVAEEALSGLGNVLWIIDLNRQSLDRVVPGVRAA
                     RLKALFQAAGWQVVEAKYGHRLQAVFAGPGGAALRRRIDDMSNEEYQALIRLDGAALR
                     ARLIEGEGNGPGRDEITRALAPIPDDELPGVIGDLGGHDLVELLRCLQAAEAEPDRPS
                     VLFAYTIKGWGLPIAGDPLNHSALLTDAQMARLRARLGVPATDEWAGFAPDSPEGGVI
                     AAARARLGESQEPTAPPVAAASIPAAIGVRHPNGTSTQEALGRILPRVGELPEAGARL
                     VTVAPDVATSTHLGGWINRAGIFAPSEQPDYEGEGPRVLRWRQGPAGRHIELGISEMN
                     LFMALGQLGLSYELVGQHLLPIGTVYDPFICRGLDSLIYSLYVDSKMIFAGTPSGMSL
                     SPEGGAHQSSVTASLGIELPGLISFEPCFANETEWVLLEALRQCCDREHGLSSYLRLS
                     TKPIDQTLLEPALARLGEAELRRQVLEGGYRLVDRRETTPHLPAGQVVQIVTAGAMVP
                     EAVEAAARLAEEEIAANVIAVTSPSRLYRALRALRRQGVHAATTPQSALFQRLILPEE
                     RRAPLVTVHDAASHALAFLGGVYGAPVISLGFDEFGQSGARADLYQVTEIDADAITAA
                     AMHALELRESD"
     gene            2821..>3014
                     /locus_tag="VFU72_07730"
     CDS             2821..>3014
                     /locus_tag="VFU72_07730"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5423411.1"
                     /translation="MQEPTPKQELLAILWRMRADLERLVAEAGPRRLEQPGALDDWSI
                     KDVIAHLTGWRWWSVARMEAA"
BASE COUNT          408 a         1114 c         1085 g          407 t
ORIGIN      
        1 gtccgcgctg agccgcgcgc tggatgagct tggtgtggcc cacacgcgcc tgagctttct
       61 ctcctccgtc tggtcgtacg agcccgacac gcgcacctgc cactacgtcg accggctgga
      121 gagcttcttc cgcgtcgagg cgacaggcga tcctcgcgca cgcccgcccg agggctatcg
      181 ctgggtgccc gccgaggagg cggtcgcgcg cctgatgccc ggcgcgcacg cccgcgccct
      241 cgacgacctg cgcgcgtcgt cccgcgtgct gcccggcccg ctcctcctct gctgagcgcc
      301 tggacgtccg gcgccgcggc tggacaatcg cccgccaggg tgtcaatctt ggggtgtcga
      361 atcgggccgg ccgcggaaga gggggatcac agtggcggcg cacgcaaccc gttcggagcg
      421 gaccgaggag cctgatattc gtagcgccag cccggagagg ctggccgctc tggacgccat
      481 ccagcggcgc atcctctggc tggcgaccaa catcatccac cacgccaacc acctgcgtcc
      541 gaacccggac aagaccaagg tcggcggaca ccaggcgtcc tcggcctcga tcgtcaccat
      601 tctgaccgcg ctctacttcc agtacctgcg ggccggcgac cgcgtctcga tcaagccgca
      661 cgcttcgccc gccttccacg ccgtgcaata cctgctgggc aatctcgacc gcgcctacct
      721 gcccaccctg cgcgcctaca aggggctcca ggcctacccc agccgcacca aagaccccga
      781 gccggtcgat ttctccaccg gctcggtcgg gttgggcgcg gtggcgcccg tcttcgccgc
      841 gctggcgcac cagtacagcc tggcccactt cggcgcggtc agctccggcc gcttcgtggc
      901 tgtcctgggc gacgccgagc tggacgaggg gaacgtctgg gaggcggtgg ccgaggaggc
      961 gctgagcggg ctgggcaacg tcctctggat catcgacctc aaccgccaga gcctcgaccg
     1021 ggtcgtaccc ggcgtgcgcg ccgcccgcct gaaggcgctc ttccaggccg ccggctggca
     1081 ggtggtcgag gccaagtacg gccaccgcct gcaggcggtc ttcgccgggc cgggcggggc
     1141 cgcgctgcgc cgccgcatcg acgacatgag caacgaggag taccaggcgc tgatccggct
     1201 cgacggcgcg gcgctgcgcg cgcggctgat cgagggggag gggaacggcc cggggcgcga
     1261 cgagatcacg cgggcgctgg cgccgatccc cgacgacgag ctgccggggg tgatcggcga
     1321 cctcggcggg cacgacctgg tcgagctgct gcgctgcctg caggcggccg aggccgagcc
     1381 ggaccgcccc agcgtcctct tcgcctacac catcaagggc tgggggctgc cgatcgccgg
     1441 cgacccgctc aaccactcgg cgctgctgac cgacgcgcag atggcgcggt tgcgcgcgcg
     1501 cctgggcgtg ccggccacag acgagtgggc tggcttcgcg cccgattcgc cggagggcgg
     1561 ggtgatcgcg gcggcgcgcg cgcggctggg cgagagccag gagccgaccg cgccgccggt
     1621 cgcggccgcg agcatccccg cggcgatcgg cgtgcgccac ccgaacggca cctcgaccca
     1681 ggaggcgctg ggccgcatcc tgccgcgcgt gggcgagctg ccggaggccg gcgcgcgcct
     1741 ggtgacggtg gcgcccgacg tggccacctc cacccacctc ggcggctgga tcaaccgggc
     1801 gggtatcttc gcccccagcg agcagcccga ctacgagggc gaggggccgc gcgtgctgcg
     1861 ctggcgccag ggcccggcgg gccggcacat cgagctgggt atctccgaga tgaacctctt
     1921 catggcgctc ggccagctcg ggctgagcta cgagctggtt ggccagcacc tgctgccgat
     1981 cggcaccgtc tacgatccct tcatctgccg cgggctcgac tcgctgatct acagcctcta
     2041 cgtcgacagc aagatgatct tcgccggcac gccctccggc atgagcctga gcccggaggg
     2101 cggcgcgcat cagtcgagcg tgaccgcctc gctggggatc gagctgccgg gcctgatcag
     2161 cttcgagccc tgcttcgcca acgagaccga gtgggtgctc ttggaggcgc tgcgccagtg
     2221 ctgcgaccgc gagcacggcc tctccagcta cctgcgcctc tcgaccaagc cgatcgacca
     2281 gacgctgctg gagccggcgc tggcgcggct gggcgaggcg gagctgcgcc gccaggtgct
     2341 ggagggcggt tatcgcctgg tcgatcgccg cgagacgacg ccgcacctgc cggccggcca
     2401 ggtggtgcag atcgtgaccg ccggagcgat ggtgcccgag gcggtcgagg cggcggcgcg
     2461 gctggccgag gaggagatcg cggccaacgt gatcgccgtc accagcccca gccggctcta
     2521 ccgcgcgctg cgcgcgctgc gccgccaggg cgtgcacgcc gccacgacgc cgcagtcggc
     2581 cctcttccag cggctgatcc tgcccgagga gcgccgggcg ccgctggtga cggtgcacga
     2641 cgcggcctcg cacgcgctcg ccttcctggg cggggtctac ggcgcgccgg tcatctcgct
     2701 gggcttcgac gagttcggcc aatcgggcgc ccgcgccgac ctctaccagg tgaccgagat
     2761 cgacgccgac gccatcaccg ccgcagccat gcacgcgctc gaactgaggg agtctgactg
     2821 atgcaggaac caacccccaa gcaggagctg ctggcgatcc tctggcgcat gcgcgccgac
     2881 ctggagcggc tggtggccga ggcagggccg cgccgcctgg agcagccggg cgcgctggac
     2941 gactggagca tcaaggacgt gatcgcccac ctgaccggct ggcgctggtg gtcggtggcg
     3001 cgcatggagg ccgc
//
LOCUS       DASUBI010000400         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_719979, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000400 DASUBI010000000
VERSION     DASUBI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719979"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFU72_07735"
     CDS             <1..260
                     /locus_tag="VFU72_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5423412.1"
                     /translation="IERGQRAHGPAAQGAVVSPLQGTVLRVAVAPGQTVVAGDLICVI
                     EAMKMENEIHSTAAGEVVRVDVEPGQTVQIGAPLVEIEPEG"
     gene            complement(270..1178)
                     /locus_tag="VFU72_07740"
     CDS             complement(270..1178)
                     /locus_tag="VFU72_07740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5423413.1"
                     /translation="MADRLETFVAWLSPGAALAVALVMVLAGSTAQYLAGTETAVGFL
                     CFLPIGLVTWRLGRRVGILISLLAAACWLAGSLAAGAGTRPILLAWNCGARLITFLVV
                     TYLIDELHRRLEETRAAADQDFLTGIANARAFDQAMQAELLRARRTGRPFTLAYLDLD
                     NFKRINDRHGHHEGDRVLYVLAHALRDCLRQVDTVARLGGDEFGILLVETDAAGARIA
                     MRRVQLALRELPFRVTFSLGAVTFNSAPRSVEEMMRLTDDAMYTVKRAGKDGCTFRLI
                     DRSGTTPHTLRHRVARPHPGRRRVAP"
     gene            1751..2290
                     /locus_tag="VFU72_07745"
     CDS             1751..2290
                     /locus_tag="VFU72_07745"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5423414.1"
                     /translation="MTDEQVLSSRRAFDGKLIRVRVDQVRLPNGNVRSREVAEHPGAV
                     GILPVLDDGRVVLVRQFRYAVGRSLLEIPAGTREAAEAPEVTARRELIEETGYRAGSL
                     DFLLRFYVSPGWADEELHIYVARELTLGEAKPEDDESLSLRVIGPEEIAPLIAAGEIA
                     DSKSIVALLAWSGARLAAR"
     gene            2531..3262
                     /locus_tag="VFU72_07750"
     CDS             2531..3262
                     /locus_tag="VFU72_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423415.1"
                     /translation="MDRKRASGSTKRVLIVDDHPLFRQGLRRLISEGSGFTIVAEAAN
                     GHEAIREADIHRPHVTLVDIQLPGVTGLNVARILKKQHPSMHVVILSMHMDDEWLFEA
                     VRAGASAFLTKEIEAPQLIDALERVSRGEQMINTAVLSRPDLAWRVLAEFRQLTGDGE
                     DSIDTSLPNAPRLPLSVREIEVLDCVAQGLSNKEIAEELFITEQTVKNHMTSVLRKLN
                     VDDRVQAVLCAVRNGWIAIGPPTAA"
     gene            complement(3318..>4072)
                     /gene="purD"
                     /locus_tag="VFU72_07755"
     CDS             complement(3318..>4072)
                     /gene="purD"
                     /locus_tag="VFU72_07755"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5423416.1"
                     /translation="LTEGSFGEAGSAILIEECLSGPEVSVLALTDGETIYPLVPATDY
                     KRVGAGDTGPNTGGMGAFAPVPAVTAQLAARIRTEILEPTLGALRQRGIGYRGVLYAG
                     LMLTERGPQVIEFNCRFGDPETEVVLPLLEDDLLPLLLATAEGRLAKLPPPRHHSGAC
                     AGVVLASAGYPGQYATGFPISGLEDLPEGVLAFHAGTKDADGHIVTSGGRVLTLVARG
                     PDLATARRRAYDGVERVRFAGKAFRPDIADTR"
BASE COUNT          678 a         1307 c         1455 g          632 t
ORIGIN      
        1 gcatcgagcg cggccagcgc gcgcatggcc cggcggcgca gggcgcggtg gtcagcccgc
       61 tccagggaac ggtgcttcgc gtggcggttg cgccagggca gaccgtcgtc gcgggcgacc
      121 tcatctgcgt catcgaagcg atgaagatgg agaatgagat ccacagcacg gcggccggcg
      181 aggtggttcg ggtcgacgtc gaacctggcc agacggtcca gatcggcgcg ccgctggtcg
      241 agattgagcc ggaggggtga accggttcgt cagggcgcga cgcggcggcg gccggggtgg
      301 gggcgggcga cgcggtggcg cagggtgtgg ggcgtggtgc cggagcgatc gatcagtcgg
      361 aaggtgcagc catctttgcc ggcgcgcttg accgtataca tggcgtcgtc ggtcaggcgc
      421 atcatctcct cgacgctgcg tggcgcactg ttgaaggtaa ccgcgcccag gctgaacgtc
      481 acgcgaaacg gcaactcgcg cagcgcgagc tggacgcgcc gcatggcgat gcgggcgccg
      541 gccgcgtcgg tctcgaccag caggatgccg aattcatccc caccgaggcg cgccaccgtg
      601 tcgacctggc gcaggcaatc gcgcagcgcg tgggccagca cgtagagtac ccggtcacct
      661 tcgtggtggc cgtgccggtc gttgatgcgc ttgaagttgt ccagatcgag ataggcgagc
      721 gtgaagggcc ggccagtgcg acgggcgcgt aggagctcgg cttgcatggc ctggtcgaag
      781 gctcgcgcgt tggcgatgcc ggtcaggaaa tcctgatcgg cggccgcgcg ggtctcttcg
      841 agccggcggt gcagttcgtc gatcaggtag gtcaccacca gaaaggtgat caggcgcgcg
      901 ccgcagttcc aggccagcag gatcgggcgc gtacccgcgc cagccgctag tgagccggcc
      961 agccagcagg ccgcggcgag gagcgagatc aggatgccga cgcgccggcc gagccgccag
     1021 gtgacgagac cgatcgggag gaaacagaga aagcccacgg cagtctcggt gccggcgagg
     1081 tactgtgccg tgctcccggc cagcaccatc acgagcgcga cggctaatgc cgccccgggg
     1141 ctcagccatg caacgaacgt ctcgagccga tccgccatcg atcacctccg gcatgcagca
     1201 tagttgccgg attccggtgc gaacatggcc acctggtacc gtctgctccc acatggttgg
     1261 ctgtgggccg tccggcgccg ggccgcgaac ggtcgagggc ttgatgtgga cggggtgcgt
     1321 gggttggctg ttgctggagc gtcggaggac gagggaggag tgccggttgg ccgtgggaca
     1381 taaagacgcg atcccgacga gcttctcgtc tcgacgcccg ggcttcatcc gaccgcgccc
     1441 ggcattccgg cctgagcctg aacaccagac gacgctctga ctccgccacc gcgacaggtg
     1501 cctgccgctg caacctcact cccagtcgcc tgaaggtgag gtgggatcgg tcgcccgatc
     1561 cgataggcgt cgatccgtgg gatcactcct cgggaccgca cgtacacaat agcacccctc
     1621 tgccgggctg tcaatacccc gtgcgaaggg cgcatgatcg accaccgccc ccgacggtgg
     1681 aacggagacg gcgaatggtg atgcaaaacg atgcgacaga ggctaggctg tcgggcatga
     1741 ggaggggtcg atgactgacg agcaggtgct ttcctcgcgg cgggcattcg atggcaagct
     1801 gattcgggtg cgggtcgatc aggtgcggtt gcccaacggc aacgtgcgct ctcgcgaggt
     1861 ggccgagcac cccggcgcgg tcggcattct gccggtgctg gacgacggcc gcgtggtgct
     1921 ggtgcgacag ttccgctacg cggtgggacg cagtctgctg gagatccccg ctggaacgcg
     1981 cgaggcggcg gaggcgccgg aggtgaccgc ccggcgtgaa ctgatcgagg agaccggtta
     2041 tcgcgccggc tcactggatt tcctgctgcg cttctacgtc agtcccggtt gggccgacga
     2101 ggagttgcac atctacgtcg cgcgcgaact cacgttgggg gaagccaagc ccgaggacga
     2161 cgagtcgctg tcgctccgcg tcatcgggcc cgaagagatc gccccgctga tcgccgcggg
     2221 tgagatcgcc gactcgaagt cgatcgtggc gctgctggcc tggagcggcg cgcggctcgc
     2281 cgcgcgctag gtgtatttac agagtgtacg tacatatggt atgatcagtc cgtgacgaga
     2341 tgacacccgc cggtacggcg gtatgcggaa agtcccgccc ccgtgcggta cgccggtact
     2401 tccactctgt tccaaattcc catgtgcctg gggttcgcgc ggtcgtactg tggaagtacc
     2461 gacaaaggtc actcgcggcg gcgggtggcg ggctgagacg cccaacacgg gcggatcaaa
     2521 gggggacagt gtggatcgca agcgagcgtc agggagcacg aagcgggttc tgatcgtgga
     2581 tgatcacccg ctctttcggc aggggttgcg gcggctaatc tcagagggga gcggcttcac
     2641 gatcgtggcc gaagcggcca acggtcacga agcaatccga gaggccgata tccaccggcc
     2701 ccacgtgaca ctggtcgaca tccagttgcc gggagtcacc ggcctgaacg tcgcccgcat
     2761 cctcaagaag cagcatccct cgatgcacgt ggtgatcctc tcgatgcaca tggatgacga
     2821 gtggctcttc gaggcggtgc gcgccggcgc ctcggcattt ctgaccaagg agatcgaagc
     2881 gccgcagcta atcgacgcgc tggagcgtgt ctcgcgcggc gagcagatga tcaacacggc
     2941 agtcctctcg cgacccgacc tggcctggcg cgtgctggcg gagttccgcc agttgacggg
     3001 cgacggcgag gacagcatcg acacgtccct gcccaacgcc ccgcgcctgc ccctctctgt
     3061 gcgcgagatc gaagtgctcg attgtgtggc tcaggggctc tcaaacaagg agattgccga
     3121 ggagctcttc atcaccgagc agacggtcaa gaaccacatg acctcggtgc tgcgcaagct
     3181 caacgtggat gaccgcgtgc aggcggttct ctgcgccgtg cgcaacggct ggatcgccat
     3241 cggcccaccg accgcggcgt agcgcggagc gtcggcattc tccagcgggg agactgggca
     3301 ggttgttgta cctgccctta tcgcgtgtcc gcgatatccg gtcggaacgc cttgccggcg
     3361 aagcggacgc gctcgacgcc gtcgtaggcg cgccggcgag ccgtcgcgag gtcgggcccg
     3421 cgcgccacca gcgtcagcac ccggccacca ctggtgacga tgtgaccgtc ggcatccttc
     3481 gtcccagcgt ggaaggccag cacgccctca ggcaggtctt cgagcccgct gatggggaag
     3541 ccggtggcgt actggccggg atagccggcc gaggcgagca cgacaccggc gcaggcgcca
     3601 ctgtgatggc gcggcggcgg cagcttggcc aggcgcccct cggcagtcgc cagcaggaga
     3661 ggcaacaggt cgtcctccag cagcggcagc acgacctcgg tctcgggatc accgaagcgg
     3721 cagttgaact cgattacctg cggcccgcgc tcggtcagca tcagccccgc gtagagcacc
     3781 cctcgatagc cgatgccccg ctggcgcagc gcaccaagcg tcggctctag gatctcggtc
     3841 cggatgcgcg cggccagctg cgctgtcacc gccggcaccg gcgcgaaggc gcccatgccg
     3901 ccggtgttcg gcccggtgtc gcccgcgccc acgcgcttgt agtcggtggc cggaacgaga
     3961 gggtagatcg tctcgccgtc ggtgagcgcc agcaccgaga cctccgggcc gctgaggcat
     4021 tcctcgatca agatcgcgct gccggcctcc ccgaagctcc cctcggtcag ca
//
LOCUS       DASUBI010000401         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_852249, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000401 DASUBI010000000
VERSION     DASUBI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852249"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1510)
                     /locus_tag="VFU72_07760"
     CDS             complement(215..1510)
                     /locus_tag="VFU72_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423417.1"
                     /translation="MARSEQASAPATTGERGGLRAGLSRNVLVLGVASLFTDIGTEMI
                     APIRILFLVLILGAPIPIAGLIEGIAESSAALLKVWSGRLADRLERRRPLIVAGYGLS
                     GAAKPLLALVTSWPQVLALVVLDRLGKGIRGSPRDALLADSTPAAYRGKAFGFHRALD
                     TLGGAIGPLLALYILVLHGQEPRGPTLVDAVVFRQIFAVTAIPGALAVLVLLAFLRER
                     RRQRAEHAAPVAPGVPVAAVPLAALGRPFWLFTGIATLFALGNPSDAFFFLRAISLES
                     WLEGLPLVYFGYNMVYAALATPFGIVSDRIGRLPLLLAAYLAFALVYLGWSLAGSGWQ
                     IYPLFLLYGVYAAAGEGVARALVVDLAPGERRGQALGWFNGLTGLATLPANLLAGWIW
                     SLYGPGSTFLLSAVVASVAACLLLANARRIAPARVRAQA"
     gene            1674..>2080
                     /locus_tag="VFU72_07765"
     CDS             1674..>2080
                     /locus_tag="VFU72_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5423418.1"
                     /translation="MRTESHTQLIEQSLDTLWRPFTQHALGQKPMVIVEGHGYTIVDS
                     EGNEYLDAMAGLWCVNVGYGQEQLIEAGARQLRTLPYTPATRPAPPSLELALRLAEVL
                     PGDLNHIQFLNSGSEAVETALKVARQYAHQQFPG"
BASE COUNT          345 a          737 c          747 g          251 t
ORIGIN      
        1 ggccgtctac agcctcgaca cgggcgaggt gcgcttcctg taggcatggg cgccgcgcca
       61 cgatggatcc ctccactgcg gtcgggatga cagggttaat ttggggctga cgaggaggac
      121 cggcctcgtc cggtaggccg gtccctctcc ctgtcatgct gagcttgtcg aaggatccgt
      181 gctggcgcgg cacgcaacgc agtcgtcggg cagtctacgc ctgggcccgc accctcgcgg
      241 gcgcgatgcg gcgcgcgttg gccagcagca ggcaggccgc gacgctggcc acgacggcgc
      301 tgagcaggaa ggtcgagccg gggccgtaga gactccagat ccagccggcc agcaggttcg
      361 ccggcagggt ggccagcccg gtcagcccgt tgaaccagcc gagcgcctgg ccgcgccgct
      421 cgcccggcgc cagatcgacc accagcgcgc gtgccacccc ctcgccggcg gcggcataga
      481 ccccatagag caggaagagc gggtagatct gccagccgga gccggccagt gaccagccga
      541 ggtagaccag cgcgaaggcc agataggcgg cgagcagcag cggcagccgc ccgatgcgat
      601 cgctgacaat gccgaacggc gtcgccagcg cggcgtagac catgttgtag ccgaagtaga
      661 ccagcggcag cccctccagc cagctctcca gcgagatcgc gcgcaggaag aagaaggcat
      721 ccgagggatt ccccagcgcg aagagggtcg cgatgccggt gaagagccag aagggccgcc
      781 ccagcgcggc cagcggcact gccgcgaccg gtacgcccgg cgccaccggc gccgcgtgct
      841 ccgcgcgctg ccgccggcgc tcgcgcagaa aggccagcag caccagcacc gccagcgcgc
      901 cggggatggc cgtcacggcg aagatctgac gaaagacgac ggcgtcgacc agcgtcggcc
      961 cgcgcggctc ctggccgtgc agcaccagga tatagagcgc gagcaggggg ccgatggcgc
     1021 ccccgagtgt gtccagggcg cggtggaagc cgaaggcctt gccgcggtag gcagcggggg
     1081 tggagtcggc cagcagggcg tcgcgcgggc tgccgcggat ccccttgccg aggcggtcga
     1141 gcacgaccag cgccagcacc tgcggccagc tcgtcaccag cgccaagagc ggcttggccg
     1201 cgcccgagag gccgtagccg gccacgatca gcggccggcg gcgctccagg cgatcggcca
     1261 gccggccgga ccagaccttg agcagggcgg cgctgctctc ggcgattccc tcgatcagcc
     1321 cggcgatcgg gatcggggcg ccgaggatca gcaccaggaa gaggatgcga atcggcgcga
     1381 tcatctcggt gccgatgtcg gtgaagaggc tggcgacgcc cagcaccagc acgttgcgcg
     1441 agaggcccgc tctcaggccg ccgcgctcgc cggtcgtcgc cggcgcgctc gcctgctcgc
     1501 tcctcgccaa cggctcccat cccctcgcgc ggcgcacgcc gcccaggtac tccgtgatcg
     1561 ccaggggcaa attgtcgcac agggggcagg gcaggccgcg cttgacgggt ccgccgcgcg
     1621 ggcctagagt gagcctgcct tgtgactgag cggagagtgg tcggaggtca gagatgcgca
     1681 ccgaatcgca cacgcagttg atcgagcagt cgctggacac cctctggcgc ccctttaccc
     1741 agcacgcgct ggggcagaag ccgatggtga tcgtcgaggg gcacggctac accatcgtcg
     1801 actccgaggg taacgagtac ctcgacgcca tggccgggct ctggtgcgtc aacgtcggct
     1861 acggccagga gcaattgatc gaggccggtg ctcggcaact gcgcaccctc ccctacaccc
     1921 cggccacccg cccggcgccc ccctcgctgg agctggcgct gcggctggcc gaggtgctgc
     1981 ccggcgacct gaaccatatc cagttcctca acagcggctc cgaggcggtc gagaccgcgc
     2041 tgaaagtcgc gcgccagtac gcgcaccagc agtttcccgg
//
LOCUS       DASUBI010000402         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_704413, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000402 DASUBI010000000
VERSION     DASUBI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704413"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VFU72_07770"
     CDS             <1..2181
                     /locus_tag="VFU72_07770"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU5423419.1"
                     /translation="VTVPMLDEIIREAADNGTGEVIIGMAHRGRLNVMAHVLGKPYAQ
                     IIGEFMGIHHREPTSASDAGSSGWTGDVKYHLGAYRPSGGELRARITLAANPSHLEFV
                     NPVIEGMTRAAQDDRTQRGAPRQNVDACLPINLHGDSALPGEGVAAETLNLSRLAGYQ
                     TGGTVHLITNNQIGYTTEPDQSRSTAYASDLARGFEIPVVHVNGDDPEACLAAAKMAY
                     AYRQRFHKDFLVDVVGYRRWGHNEGDEPAFTQPLMYAIISKRPTVRAVWAERLEQEGV
                     IQPGQADQLLKQQMDQLQQIRGEIAASKGHREEPPTPMRTTAHVRPAETAVPRELLEK
                     LNQEIHALPEGFTLNPKLQRNMSQRAAAVAKGGPIDWAHAEDLALASILYDSTPVRMT
                     GQDVERGTFSQRHLIFHDINTGKLNVPLQTMPDAHASFAIYNSPLSEAAPLGFEYGYS
                     VHAPEAMVVWEAQFGDFANAGQVIVDQFIAAGRAKWDQISSLVLLLPHGYEGQGPEHS
                     SARLERYLQLCANDNLRVANCTTSAQYFHLLRKQAALLNRAPRPLILMTPKVLLRHPL
                     AASPVEEFTSGQFQPVLDDPNIQDRQTVTRVVACSGKVYVDLAGSKERAETRDVAVIR
                     LELLYPFPEENLRALLSSYPNARQLIWLQEEPRNMGAWTFVHPWLEAAAGDQLEVVYV
                     GRPERASPAEGWAELHRSEQDRIVHEALTGAPVMRESYGVKHGD"
     gene            2171..>3353
                     /locus_tag="VFU72_07775"
     CDS             2171..>3353
                     /locus_tag="VFU72_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5423420.1"
                     /translation="MATEVRVPQLGESVVDAVVGTWLKQEGQPVAVGEPLVELETDKV
                     NVEVSAEEAGVLQQILKPEGETVGVGEVIATIAAGAAVAAQPQAAQPAAQQASPQPAE
                     QAAAAVAPAGAFPPPAENVAAAPPGVPSPAGGAVPAAEPAGSLRASPAVRRLAEEHGI
                     DLGQVAGTGENGRVSRDDVMRHVLSRQQPAAAAPAAAPTPAPAPAARPGTAPVPAMPQ
                     LELPEVLSQTNHQREERVRMSRRRQTIAQRLQSVQLEAAMLTTFNEVDMQAVMDLRKR
                     WRDIFKERHGVSLGYMSFFTKAVVGALKAFPYLNAEIQGNEFVLKHYYDIGIAVGTDD
                     GLVVPVVRDAERKSFAQIEQDILDLATKARDGKLTLEDLMGGTFTITTGGIYGSLNST
                     PI"
BASE COUNT          573 a         1218 c         1126 g          436 t
ORIGIN      
        1 gtcacggtcc cgatgctcga cgagatcatc cgtgaggcgg ccgacaacgg caccggcgag
       61 gtgatcatcg gcatggccca ccgcggccgg ctgaacgtga tggcgcacgt gctgggcaag
      121 ccctacgcgc agatcatcgg cgaattcatg ggcatccacc accgcgagcc gacctcggcc
      181 agcgacgccg gctcctccgg ctggaccggc gacgtcaagt accacctggg cgcctaccgg
      241 ccctccggtg gggagttgcg cgcccgcatc acgctggccg ccaaccccag ccacctggag
      301 ttcgtcaatc cggtcatcga ggggatgacg cgcgcggcgc aggacgaccg cacccaacgc
      361 ggtgctccgc gccagaacgt cgacgcctgc ctgccgatca acctgcacgg cgactccgcc
      421 ctccccggcg agggtgtggc ggccgagaca ctcaacctct cgcgcctggc cggctatcag
      481 accggcggca cggtccacct gatcaccaac aaccagatcg gctacaccac cgagccggac
      541 cagagccgct cgaccgccta cgccagcgac ctggcgcgcg gcttcgagat ccccgtcgtc
      601 cacgtcaatg gcgacgaccc cgaggcctgc ctggcggccg cgaagatggc ctatgcgtac
      661 cgccagcgct tccacaagga cttcctggtc gacgtggtcg gctaccgccg ctggggccac
      721 aacgagggcg acgagccggc cttcacccag ccgctgatgt acgccatcat cagcaagcgg
      781 cccacggtgc gcgccgtctg ggccgagcgg ctggagcagg agggggtcat ccaaccgggg
      841 caggccgacc agctcctcaa gcagcagatg gatcaactgc agcagattcg cggggagatc
      901 gccgccagca agggacaccg cgaggagccg ccgaccccga tgcgcaccac cgcgcatgtg
      961 cgtccggccg agaccgccgt gccgcgtgag ctgctggaga agctgaacca ggagatccac
     1021 gccctgcccg agggcttcac cctcaatccc aagctgcaac gcaacatgtc gcagcgcgcc
     1081 gcggccgtgg ccaagggcgg gccgatcgac tgggcgcacg ccgaggatct ggccctggcc
     1141 agcatcctct acgacagtac gccggtgcgc atgaccgggc aggatgtcga gcgcggcacc
     1201 ttcagtcagc gccacctgat cttccacgac atcaacacgg gcaagctgaa cgtgcccctc
     1261 cagacgatgc ccgacgcgca cgcctccttc gccatctaca acagcccgtt gtcggaggcc
     1321 gcgccgctcg gcttcgagta cggctatagc gtccacgcgc cggaggcgat ggtcgtctgg
     1381 gaggcgcagt tcggcgactt cgccaacgcc ggccaggtga tcgtcgatca gttcatcgcc
     1441 gccgggcgcg ccaagtggga tcagatcagc tcgctggtgc tgctcctgcc gcacggctac
     1501 gaaggccagg gaccggagca ctccagcgcc cggctggagc gctacctgca gctctgtgcc
     1561 aacgacaacc tgcgcgtggc caactgcacc acctcggccc agtacttcca cctgctgcgc
     1621 aagcaggcgg cgctgctcaa ccgcgcgccg cgcccattga ttctgatgac gccgaaggtg
     1681 ctgctgcgcc acccgctggc cgcctcgccg gtcgaggagt tcaccagcgg ccaattccaa
     1741 ccggtgctcg acgacccgaa tatccaggac cgccagaccg tcacgcgcgt ggtagcctgc
     1801 agcggcaagg tctacgtcga cctcgccggc tccaaggagc gggcggagac cagggacgtg
     1861 gccgtgatac ggctggagct gctctacccc ttcccggagg agaacctgcg tgccctgctg
     1921 agcagctatc ccaatgcgcg ccagctcatc tggctgcagg aggagccgcg caacatgggc
     1981 gcctggacct tcgttcaccc ctggctggaa gccgcggccg gcgatcagct cgaggtggtc
     2041 tacgtgggcc ggccggagcg cgccagcccg gccgagggct gggccgagct gcaccggtcg
     2101 gagcaggacc gcatcgtgca cgaggcgctc acgggcgcgc cggtgatgcg ggagtcgtac
     2161 ggagtgaagc atggcgactg aggtgcgggt gccgcagctc ggcgagtcgg tcgtcgacgc
     2221 ggtggtcgga acctggctca aacaggaggg gcagccggtc gcggtggggg agccgctggt
     2281 cgagctggag accgacaagg tcaatgtcga ggtctcggcc gaagaagcag gcgtcttgca
     2341 gcagattctg aagccggagg gcgagacggt cggcgtcggc gaggtgatcg ctaccatcgc
     2401 ggcgggcgcg gcagtcgccg cccagcctca ggcggcccag ccggcggcgc agcaagcgtc
     2461 gccacagccg gccgagcaag cggcagccgc ggtggcgcca gccggcgcct tcccgccacc
     2521 ggccgagaac gtggccgccg cgccccccgg cgtgcccagc cctgcgggag gcgcggtgcc
     2581 ggccgccgag cccgccggat ctctgcgcgc cagcccggcc gtgcgccggc tggccgaaga
     2641 gcacggcatc gacctgggcc aggtcgccgg taccggcgag aacgggcgcg tctctcgcga
     2701 cgacgtgatg cgccacgtcc tctcgcgcca gcaaccggcg gccgctgcac cggccgcggc
     2761 gccaaccccc gccccggctc cggctgcccg cccggggacg gcgccggtcc cggcgatgcc
     2821 gcagctcgag ctgccggaag tgctctcgca gacgaaccac cagcgcgaag agcgcgtgcg
     2881 catgagccgc cggcgacaga cgatcgccca gcggctgcaa tcggtgcagc tggaagccgc
     2941 catgctgacg accttcaacg aggtcgacat gcaggcggtg atggacctgc gcaaacgctg
     3001 gcgcgacatc ttcaaggagc gccacggcgt cagcctgggc tacatgtcct tcttcaccaa
     3061 ggcggtcgtc ggcgcgctca aagccttccc ctacctcaac gccgagatcc agggcaacga
     3121 gttcgtgctc aagcactact acgacatcgg catcgccgtc ggcaccgacg acgggctggt
     3181 cgtgccggtg gtgcgcgacg ccgagcgcaa gtcgttcgcc cagatcgagc aagacatcct
     3241 cgatctggcc accaaggcgc gcgacggcaa gctgacgctg gaagatctga tgggcggcac
     3301 cttcactatc accaccggcg gcatctacgg ctcgctgaac tcgactccga tcc
//
LOCUS       DASUBI010000403         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_92477, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000403 DASUBI010000000
VERSION     DASUBI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92477"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..381
                     /locus_tag="VFU72_07780"
                     /pseudo
     CDS             1..381
                     /locus_tag="VFU72_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..1271)
                     /gene="istB"
                     /locus_tag="VFU72_07785"
     CDS             complement(489..1271)
                     /gene="istB"
                     /locus_tag="VFU72_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEU5423421.1"
                     /translation="MELQQQLSPMLKQLRLSGVLATLEARHRQALEGQWSYIEFLERL
                     LEDEVERRAQKQLALRIRRGSLNTTKTLEGYDFGFNPSLNRQQVLQLAACDYIRRRRN
                     LLICGPTGVGKSHLAQALAHEAARQGFEVLFVNTHKMLQHLHGGRADGSWERRLAQYL
                     RPDLLVLDDFGLKPLTDPAPSDLYDVINERYEVGSILVTSNRAPAEWPDLFGNPLLAS
                     AGLDRLAHHAETLVITGRSYRAQGQVGDGRELLKEKGGATTQ"
     gene            complement(1250..2815)
                     /gene="istA"
                     /locus_tag="VFU72_07790"
     CDS             complement(1250..2815)
                     /gene="istA"
                     /locus_tag="VFU72_07790"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEU5423422.1"
                     /translation="MAGRSIDLMDVREVLRHLQTTTNLSAIQRATGLNRRTLMRYRAW
                     ATAQGLLTRPLPPLDELQRLAAQTLTPPAPPQTVSTVEPYRDLVRDLHARGVEGAAIW
                     QRLRERGYRGSLSSVYRFLRQLDPPAPDVTVRVERAPGSEAQVDFGYAGYLVDNASGQ
                     RRKAWAFVMTLAYSRHQYVEFVHDQTLPTWITLHRHAFAFFGGVPQRIVLDNLKAGIV
                     RACLDDPQIQPTYRECAEHYGFLVAPCAPRTPEHKGKVEQGGVHYVKRNFLGGRAPTT
                     LAQANADVRVWCQSTAGLRRHGTTKEIPLERFAAVERVQLLPLPPAPYDLAVWKQVTL
                     HRDCHVVFAQAFYSAPFRLVGQRLWVRGGSQDVRLYTADYQLVATHPRAARPGERQTH
                     PDHLPQAKLPQVLWNRELCQALAAEVGPATTTVTQQLLADRVVDRHSRVVRILRLRDT
                     VGDQRLEAACARALHYGDLTYVTIKHILAHGLEAADRPPAVAPAPARTFVRTAAELLG
                     HVFGGLTWNSSSN"
BASE COUNT          454 a         1020 c         1019 g          466 t
ORIGIN      
        1 gaggtgctcc tggcgcgcag cctcttgagc gggacggccg acgacctcgc cgccctactc
       61 cgcgaggtcc gcgacgcgct gcccgtcctg gtcaccggcg tcatctccga tgggcagcac
      121 cccatccgcg aggccgtgcg gcgcgagttg cccgaggcag cgcaccagct ctgccagttc
      181 cactacctcc gcgaggcggc caagccggtc ttcgaggccg accgccacgc caagaagctg
      241 ctcaagaagg gggtgcgcgg ggtgcgcccc atcgagcggg cgctggaagg ccgggaaggc
      301 gcggaggcgg acgcggtccg gggctactgc ctggcggtgc gcagcgcgct gaccgacgat
      361 ggccgcccac cgctcgctgc ggcggtgtca atcgtcgccc ataactgcac aacgatcggc
      421 ggcaatagcg cacacggatc ggcggcaatg ctgcacaacg cggggccagc cgcccacacc
      481 gtccaccgct actgcgtcgt cgcacctcct ttctctttaa gcaattctcg tccgtccccg
      541 acctgcccct gtgcgcggta actccgcccg gtaatgacga gcgtttcggc gtggtgggcc
      601 aggcggtcga ggccggcgga ggccaggagc ggattgccga acaggtcggg ccactcggcc
      661 ggcgcccgat tactggtcac caggatgctg cccacctcgt accgttcatt aatgacgtca
      721 tacaagtcgc tcggggccgg gtcggtcagc ggcttgaggc cgaagtcatc gagcaccagc
      781 aggtccgggc gcaggtactg cgccagccgg cgctcccagc tcccgtcggc ccggccgcca
      841 tgcaggtgtt gcagcatctt gtgggtattg acgaagagca cctcgaagcc ttgccgggcg
      901 gcctcatggg ccaacgcctg ggccaagtga ctttttccca cccccgtcgg gccgcagatc
      961 agcagatttc gccggcggcg gatgtagtcg cacgcggcca gctgcaacac ctgctggcga
     1021 ttgaggctgg gattgaagcc gaagtcgtag ccctccaggg tcttggtggt gtttaggctg
     1081 ccgcggcgga tgcgcagcgc gagttgcttc tgcgcccgcc gttccacctc atcctccagg
     1141 aggcgctcga ggaactcgat atacgaccac tggccctcaa gggcttgccg gtggcgggct
     1201 tccagcgtgg cgagcacccc ggagaggcgc agttgcttga gcatcgggct caattgctgc
     1261 tggagttcca tgtcagccct ccaaagacat gccccaacaa ctccgccgcc gtgcgcacga
     1321 acgtgcgcgc tggggcgggc gcgacggccg gtggacggtc ggcggcctcc aacccgtgcg
     1381 ccaggatgtg cttgatcgtc acgtaggtca ggtcgccgta gtgcagggcc cgggcacagg
     1441 cggcttcgag ccgctggtcg ccgaccgtgt cgcggaggcg gaggatgcgc acgacgcggc
     1501 tgtgtcggtc gaccacgcgg tcggccaaca gctgctgcgt gacggtggtc gtcgcggggc
     1561 cgacctcggc cgctagcgcc tgacacagct cgcggttcca gagcacctgc ggcagtttcg
     1621 cctggggcaa gtgatccgga tgggtctgcc gctctcccgg gcgcgcggcg cgcgggtgag
     1681 tcgcgaccag ttgatagtcg gccgtgtaga ggcgcacgtc ctggctgccg ccccgcaccc
     1741 acagccgctg cccgaccagg cggaacggcg cggagtagaa ggcctgtgcg aagacaacgt
     1801 ggcagtcacg atggagcgtg acctgcttcc agaccgccag gtcgtagggc gccggcggca
     1861 gcgggaggag ctgcacccgc tcgacggcgg cgaaccgctc gagcgggatc tctttcgtcg
     1921 tgccgtggcg gcgcagcccg gcggtgctct gacaccagac gcgcacatcc gcattcgctt
     1981 gcgccagcgt cgtcggggcg cggccaccga ggaagttgcg cttgacgtag tggacgccgc
     2041 cctgctcgac cttgcccttg tgttccggcg tgcggggcgc acagggcgcc acgaggaagc
     2101 catagtgctc cgcgcattcc cgatacgtcg gttgaatctg gggatcgtcg aggcacgccc
     2161 ggacgatgcc cgccttcagg ttatccagca cgatccgctg cggcacgccg ccgaagaagg
     2221 cgaaggcgtg ccgatggagc gtgatccagg tcggcagcgt ctggtcatgg acgaactcca
     2281 cgtactgatg gcgactgtaa gccagcgtca tcacgaaggc ccaggccttg cgccgctgcc
     2341 cgctcgcgtt gtccaccaga tagcccgcgt agccgaagtc gacctgcgcc tcgctgccgg
     2401 gggcccgctc cacccgcacg gtcacgtccg gcgcgggcgg atcgagctgg cgcagaaacc
     2461 ggtagaccga cgagagactc ccacggtagc cccgctcgcg cagccgctgc cagatcgccg
     2521 ccccttccac accgcgggcg tggaggtcgc gcaccaggtc acgatacggc tcgaccgtgg
     2581 agaccgtctg gggcggggcc ggcggggtca gcgtctgcgc ggccagccgc tgcaactcgt
     2641 ccagcggggg caggggcctg gtgagcaggc cctgcgccgt cgcccaggcc cggtagcgca
     2701 tcagcgtccg ccgattcagc cccgtcgccc gttgaatcgc gctgagattg gtggtcgttt
     2761 gcagatgtcg aaggacttcc cgcacatcca tcaggtcgat gctccttccc gccatacgcc
     2821 ctcctggctg ctccgaattc cgcgctgcca ggaggatagt cacacggcgt ggagggcccc
     2881 cactcgcctt gtgcaggatt gccgccgatt gtgcgctatg gccgatgact actgtgcact
     2941 ataccccgac gaatgacag
//
LOCUS       DASUBI010000404         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_928149, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000404 DASUBI010000000
VERSION     DASUBI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928149"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFU72_07795"
     CDS             complement(<1..126)
                     /locus_tag="VFU72_07795"
                     /inference="COORDINATES: protein motif:HMM:NF045310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU5423423.1"
                     /translation="MRSRVTAEPVAKLIDEFAKLPGIGPKTASRLAYFVLRSSRED"
     gene            complement(193..>2296)
                     /locus_tag="VFU72_07800"
     CDS             complement(193..>2296)
                     /locus_tag="VFU72_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU5423424.1"
                     /translation="PRGRRLAWLGRAGLALVRLVGRLRFVPRLGLPERLRRELLALAA
                     LTGAALVTVPLLGLTQQVSLAGLVSDGLRAVFGAGAFLVPLALVWLAGEALFGGEGRA
                     RRALGAVLLLAVAVTYLDLLDAAGRLGGYLGAALGSLTMEVAGRLGAGILVFSLALAA
                     LYLLTGADARGVIESWRAIRARRAERRSARLAAVPSEPSPAPEPEPAPAPEPLRPVIK
                     VPQRAARAQDDNPITAPTGPLPLPEIGKLALYEGGAPNELELEGKAERIEETLGNFKV
                     DARVREVYPGPAVTLFALEPGPGVKVKRITELQNDLALALAAHSIRIEAPVPGKARVG
                     IEIPNSSVHTIGLRETLEASEFQSSKARVPLALGRDVNGDYVVADLARMPHLLIAGST
                     GSGKSVAINSIIATFLLTRQPEELQMVLVDPKMVELVGYNGVPHLKCPVVTEMDKVVG
                     TLRLVVKEMEDRYQRFSSLGVRNIDGYKLKRQDDPSLEELPYLIVIIDELADLMMTTP
                     EEVETLLVRLAQMARATGIHLLIATQRPSVDVLTGLIKTNIPARIAFAVTSLTDSRVI
                     LDLPGAERLLGRGDMLFLPPDAAKPQRVQGSYIEDRDLQYVVRHWQRVRPDFDYDPEW
                     QVTADEEGEGGDDPLMEQALKVVRQHGTASASMLQRRLRIGYNRASRLIEQMEDEGIV
                     GPADGSRGRPVYLDEDDS"
BASE COUNT          364 a          837 c          766 g          329 t
ORIGIN      
        1 atcctcccgc gagctgcgca agacgaaata ggccaggcga gaggccgtct tggggccgat
       61 tcctggcagc ttggcaaact cgtcgatgag cttcgcgacc ggctccgccg ttacccgaga
      121 gcgcacgcaa tcctcacgtc ctgcacctcg cttccgggcc aaattatacc aaacgcttgg
      181 taattggccg gtttagctgt cgtcctcgtc gaggtagacg gggcgcccgc gtgaaccgtc
      241 ggccggtccc acgatgccct catcctccat ctgctcgatg agccgcgagg cccggttgta
      301 gccgatgcgc aggcgacgct gcagcatgga ggccgaagcc gtgccgtgct ggcgcacgac
      361 tttgagcgcc tgctccatca gcgggtcgtc gcccccctcg ccttcctcgt cggccgtgac
      421 ctgccactcc ggatcgtagt cgaagtcggg ccgcactcgc tgccagtggc gcacgacgta
      481 ctgcaggtca cgatcttcga tgtaggagcc ctgcacgcgc tgcggcttgg ccgcgtcggg
      541 cggcaagaag agcatgtcac cgcggcccag caaccgctcg gcgcctggga gatcgaggat
      601 gacccggctg tcggtcagcg aggtgacggc gaaggcgatg cgcgccggga tgttggtctt
      661 gatcaggccg gtcagcacgt cgaccgaggg ccgctgggtg gcgatcagca ggtggatgcc
      721 ggtggcgcgc gccatctgcg ccaaccgcac cagcagcgtc tcgacctcct ccggtgtggt
      781 catcatcagg tcggccagct cgtcgatgat cacgatcaga tagggcagct cttccagcga
      841 ggggtcatcc tggcgcttga gcttgtaacc gtcgatattg cgtacgccca gcgacgagaa
      901 gcgctggtag cggtcctcca tctccttgac caccaggcgc agcgtgccaa cgaccttgtc
      961 catctcggtg acgaccgggc acttcaggtg cggcacgccg ttgtagccca ccagctcgac
     1021 catcttgggg tcgaccagca ccatctgcag ctcctccggc tgacgcgtca gcaggaacgt
     1081 ggcgatgatc gagttgatcg cgaccgactt gcccgagccg gtggagccgg cgatcaggag
     1141 gtgcggcatg cgggccagat cggcgaccac gtagtcgccg ttgacgtcgc gccccagcgc
     1201 cagcggcacc cgcgccttgc tcgactgaaa ctcggaggcc tccagagtct cgcgcaagcc
     1261 gatggtgtgg acgctgctgt tggggatctc gatgccgacg cgcgccttgc ccggcaccgg
     1321 cgcctcaatg cggatcgagt gcgccgccag ggccagcgcc aggtcgttct gcagctcggt
     1381 gatgcgcttg accttgacgc ccgggccggg ctccagtgcg aagagggtca ccgccggacc
     1441 aggatagacc tcgcgtaccc tcgcgtcgac cttgaagttg ccgagcgtct cctcgatgcg
     1501 ttccgccttg ccctcaagct ccagctcgtt gggcgcgccc ccctcgtaga gcgccagctt
     1561 gccaatctcc ggcagcggca gagggccggt gggcgcggtg atcgggttat cgtcctgcgc
     1621 gcgcgccgcg cgctggggca ccttgatcac cggccgcagc ggctcgggag cgggcgcagg
     1681 ctccggctcg ggtgcgggtg acggttcgct aggcacggca gccaagcgcg ccgagcgacg
     1741 ctccgcacgc cgggcacgga tcgctcgcca gctctcgatc acgccgcgcg cgtcggcgcc
     1801 ggtcaacagg tagagcgcgg ccagcgccag gctgaagacc agaatacccg cgcccagccg
     1861 cccggccacc tccatcgtca gcgagccgag cgcggcgccg aggtagcccc caagccggcc
     1921 ggccgcatcc agcaggtcga gataggtaac ggcgaccgcc agcaggagca cggcgccgag
     1981 cgcgcgtcgt gcgcgaccct cgccgccgaa gagcgcctcc cccgccagcc agaccagcgc
     2041 gagcggcacc aggaacgcgc ccgcgccgaa gacggcgcgc agcccgtcgc tcaccagtcc
     2101 cgccagcgaa acctgctgcg tcagcccgag gagcggcacc gtgaccagcg ccgcccccgt
     2161 cagcgccgcc agcgccagca actcgcgccg cagccgctcg ggcaggccaa gccgcggcac
     2221 gaagcgcagc cggcccacca gccgcaccaa cgccagcccg gcccgcccca accaggccag
     2281 gcgccgcccg cgcggc
//
LOCUS       DASUBI010000405         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_301497, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000405 DASUBI010000000
VERSION     DASUBI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301497"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU72_07805"
     CDS             <1..197
                     /locus_tag="VFU72_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5423425.1"
                     /translation="TRSGASWLLRERHTGSFDRQVTLPTPVNAEQAQAEFHNGLLTIR
                     LPKSEQARPQTITIGSTQAP"
     gene            204..1190
                     /locus_tag="VFU72_07810"
     CDS             204..1190
                     /locus_tag="VFU72_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU5423426.1"
                     /translation="MIPLLAADRIDATLVSADQLTAHLRGLAERAAGPIFSILLIVAV
                     TFLLLRGLRTIVRQLMKRVLERSDEPPRELALRVSTLSSVVESAGRMIILVVAGMTVL
                     TNLGLDIGPLIASAGVAGIAIGLGAQSLIRDLIGGFFILFEDQFGVGDVISVNGQSGT
                     VELFSLRRTGLRAADGAMITVPNGDLRTVKNQTKDWSRAVIDVDISYDDDVDRAIAVL
                     EEMVSGLEHDPAIGGALTGPVEVLGVQALGAYQVTIRIMAKTRPMQQWSVERELRRRV
                     KRLLQEKGITIPYPHSVTLLRPAATSADQPAGALVVQGGGEPDERRTPGDGA"
     gene            1424..>2120
                     /locus_tag="VFU72_07815"
     CDS             1424..>2120
                     /locus_tag="VFU72_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423427.1"
                     /translation="MGQENRLRELMDGAMSGRLSRRDVLRRAALLGLSAPVVGALLAA
                     CGGSSNNATSGAGAATSTTAPTSAAGAATATRPAAATSTTAAASPAASPVVVASPSAT
                     GSPAATAFDKNKAPAVPNPQAAKQYSGQKITYYGDSVGAGHDIDMVLTGQFTKDTGIE
                     VNVVPHPQSSDDTYAQYLRFFQGKSSDMDVMMLDVIWPGALAPHLHDLTAKLGTEAKQ
                     HYSTIIANNTVNGK"
BASE COUNT          353 a          778 c          698 g          291 t
ORIGIN      
        1 tgacccgctc cggcgccagc tggctgctgc gtgagcgcca caccggctcc ttcgatcgcc
       61 aggtgacgct gccgaccccg gtcaacgccg agcaggcgca ggccgagttt cacaacgggc
      121 tcttgaccat tcgcctgccg aagtctgagc aagcccggcc ccagaccatc actatcggca
      181 gcacccaggc accctagcgc cgcgtgattc ccctgctcgc cgccgaccgg atcgatgcca
      241 cgctggtcag cgccgaccag ctcaccgcgc acctgcgcgg cctggccgag cgcgccgccg
      301 gaccgatctt ctcgatcctg ctgatcgtcg ccgtcacctt tctgctgttg cgcggcctac
      361 gcaccatcgt gcgccagttg atgaagcgcg tgctggagcg ctccgacgag ccgccgcgcg
      421 agctggcgct gcgcgtcagc accctctcca gcgtggtcga gtcggccggc cggatgatca
      481 tcctggtcgt ggccggcatg acggtcctga ccaacctggg gctcgacatc ggcccgctga
      541 tcgccagcgc cggagtggcc ggcatcgcca tcggcctggg cgcccagagc ctgatccgcg
      601 acctgatcgg cggcttcttc atcctcttcg aggaccagtt cggcgtcggc gacgtgatca
      661 gcgtcaacgg ccagagcggc accgtcgagc tcttcagcct gcggcgcacc gggctgcgcg
      721 ccgccgacgg cgccatgatc acggtgccca acggcgatct gcgcacggtc aagaatcaga
      781 ccaaggactg gtcgcgcgcg gtcatcgacg tcgatatctc ctacgacgac gacgtcgatc
      841 gggccatcgc ggtactggag gagatggtca gcggcctgga gcacgacccc gccatcggcg
      901 gcgcgctgac cggcccggtc gaggtgctgg gcgtgcaggc gctcggcgcc tatcaggtga
      961 cgatccggat catggccaag acccggccga tgcagcagtg gagcgtcgag cgtgagctgc
     1021 gccgccgcgt caagcgcctg ctgcaggaga agggcatcac catcccctat ccccacagcg
     1081 tcaccctgct gcgcccggca gcgaccagtg ccgatcagcc ggccggcgcg ctcgtcgtgc
     1141 aaggcggagg cgaaccggac gagcgccgga cgcccggcga tggagcctaa gcccggccga
     1201 tccatcagat gcgtcagatg accgtcggcc cgcatattgc tagggctgcg cgcgataagc
     1261 gatcgggggg aaggcagtgg aacggtagga gcctacccgc cttgtcttgg tcgcgatgac
     1321 cgggattcgc tcgacgcgca ggggtgaacc gcagtggtgg ctacccacgc gcacgacgga
     1381 ttggtccact gttcgggtgg agcatttcga ggaggagccc agtatgggtc aggagaaccg
     1441 tctccgggag ttgatggacg gcgccatgag cggccgcttg agccggcgcg acgtgctgcg
     1501 ccgcgcggcg ctgctcgggc tgagcgcgcc ggtggtgggc gcgctgctgg ccgcctgcgg
     1561 tggcagcagc aacaacgcca cctccggggc cggcgcggcc accagcacca ccgctcccac
     1621 cagcgcggcc ggcgccgcca ccgcgacccg cccggccgcg gccaccagca ccaccgcggc
     1681 ggccagcccg gcggccagcc cggtcgtcgt agccagcccc agcgccaccg gctcgcccgc
     1741 ggccaccgcc ttcgataaga acaaggcgcc cgccgtgccg aatccccagg cggccaagca
     1801 gtacagcggc cagaagatca cctactacgg cgactcggtc ggcgccgggc acgacatcga
     1861 catggtgctc accggccagt tcaccaagga caccggtatc gaggtcaacg tggtgccgca
     1921 cccgcagagc tcggacgaca cctacgccca gtacctgcgc ttcttccagg gcaagtcgtc
     1981 cgacatggac gtgatgatgc tcgacgtgat ctggccgggc gccctcgcgc cgcacctgca
     2041 cgacctcacg gccaaactgg ggaccgaagc caagcagcac tacagcacca tcatcgccaa
     2101 caacaccgtc aacggcaaac
//
LOCUS       DASUBI010000406         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1200377, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000406 DASUBI010000000
VERSION     DASUBI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200377"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFU72_07820"
     CDS             <1..593
                     /locus_tag="VFU72_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEU5423428.1"
                     /translation="GPKWAAFAKFVGATGEGVRQAGHVLKRGDPRVIAGSLGYWFFDN
                     LVLWATFHAIGTTPPFTVVLMAYLIGQLGGLLPIPGGIGGIDGGLIGTFIVYGVAAAP
                     TAAAVLAYRLILFWLPLAVGAVAFGLGVAAGLAVPESDQEDKLFGATSDKVTGRAEQM
                     ASQAVDKAEQKVESTLQDLSGTSSSQRSQQSSGASS"
     gene            764..1234
                     /locus_tag="VFU72_07825"
     CDS             764..1234
                     /locus_tag="VFU72_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423429.1"
                     /translation="MSQRDASDRPPRAGEGDGEGDGQAVTPGQPQPGAPAGAQAPRAE
                     PVDRVVAEHSQGPGRSLQAAAARATAAKQQASQALGSAAASLRQQAGTLPGGQPVHQA
                     ARAVAGTLQNASQGLQQQHVGGMMQQLEQQVREQPLLSLGIAFLAGMILGRLSD"
     gene            complement(1231..2283)
                     /gene="mnmA"
                     /locus_tag="VFU72_07830"
     CDS             complement(1231..2283)
                     /gene="mnmA"
                     /locus_tag="VFU72_07830"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5423430.1"
                     /translation="MARIIVALSGGVDSAVAASLLKEAGWEPVGVHLRLYDADVEADG
                     ACCGDLAAADARRVAAQLDIPFYVRDLRDDFAREVAAFTVASYARAETPNPCLACNHR
                     VRIPGLLRLADSLGIEAVATGHYVRILEHDGRRFLAEGREPGRDQSYALYRLGSEELA
                     RLHFPLGALDKDAVRERAAAAGLHVADKASSVDLCFAKSAGGIGRLVARERPETGQPG
                     PLVDEQGAVVGEHPGIAYLTIGQRSGLHWRRTTPERRYVAGIDPTTRTATVAPRPRLG
                     TRAATLRDPIWHDTPADGVEARLRYQGPRVPVVVTGDTVCFPEPGPPLAVGQGVVLYD
                     GPRVLGGGVAAAVERG"
     gene            2574..>2952
                     /locus_tag="VFU72_07835"
     CDS             2574..>2952
                     /locus_tag="VFU72_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423431.1"
                     /translation="MVYLSEQVVSLELAQRLKALGVEQESMYWWQEDGPGTGHWAILQ
                     FPASTFLSPAGPVELDKTAAFNVSELGRLWPELARSYRRVDGQWQAEAPLELAEARGW
                     GWDEPRHAAGATEADARAALLIQL"
BASE COUNT          401 a         1035 c         1136 g          380 t
ORIGIN      
        1 gcggcccgaa gtgggctgcg ttcgcgaagt tcgtcggcgc gaccggcgag ggcgtgcggc
       61 aggcgggcca cgtgctcaag cgcggtgacc cgcgcgtgat cgccggatcg ctgggctact
      121 ggttcttcga caacctcgtg ctgtgggcga cgttccacgc catcggcacg acgccgccgt
      181 tcaccgtggt cctgatggcg tacctgatcg gccagctcgg cggcctcctc cccatccccg
      241 gcgggatcgg cggcatcgac ggcggcctga tcgggacgtt catcgtctac ggcgtggcgg
      301 ctgcgcccac cgccgcggcg gtcctcgcct accgcctgat cctcttctgg ctgccgctgg
      361 ccgtcggcgc ggtcgccttc ggcctgggcg tcgcggccgg gctggccgtg ccggagagcg
      421 accaggagga caagcttttc ggggccacca gcgacaaggt gaccggccgg gccgagcaga
      481 tggcctcgca ggcggtggac aaggccgagc agaaggtcga aagcacgctg caagacctca
      541 gcggcacctc gtcgagccag cgcagccagc agagctcggg cgcctcgtcg tagccgggcg
      601 ttcgtccggc gccacggcga ccatccgtcc tcacccgccg ggagcgtggc tccgcggcgg
      661 gtgtcttttc acgccgcttc tggcatgatg gacgcggtcg tgtgacaacg agtccagata
      721 cgcagcgccc cggctcagcc gggcgacgac acgggagttg agcatgagcc agcgagacgc
      781 atcggatcgc ccgccgcgcg cgggagaggg tgacggtgaa ggcgacgggc aggccgtgac
      841 tccggggcag ccgcagccgg gagcgccagc aggggcgcaa gcgccgcgag cggagccggt
      901 ggaccgggta gtcgcggagc actctcaggg gccgggccgc tcgttgcagg ccgccgcggc
      961 gcgcgccacc gccgccaagc agcaggccag ccaggcgctc ggctcggccg ccgcatcgct
     1021 gcgccagcag gccggcacgc tccccggcgg ccagcccgta caccaggcgg cgcgcgccgt
     1081 cgccggcacg ctgcagaatg cctcgcaggg actgcagcag cagcatgtgg gagggatgat
     1141 gcagcagttg gagcagcagg tgcgcgagca gccgctgctg tcgctgggga tcgccttcct
     1201 ggcggggatg atcctcgggc gcctgagcga ctagccgcgc tcgaccgccg cggccacgcc
     1261 gccgccgagc acgcgcgggc cgtcgtagag caccacgccc tggccgaccg ccagcggcgg
     1321 cccgggctcg gggaagcaca cggtgtcgcc cgtgacgacg accggcacgc gcggtccctg
     1381 gtagcgcagg cgcgcctcga cgccgtccgc cggggtgtcg tgccagatcg ggtcgcgcag
     1441 cgtggccgcg cgcgtgccca ggcgcggccg cggcgccacc gtcgccgtgc gcgtggtggg
     1501 gtcgatgccg gccacgtagc ggcgctcggg cgtggtgcgg cgccagtgga gcccgctgcg
     1561 ctgcccgatc gtcaggtagg cgatgccggg gtgctcgccg accaccgcgc cctgctcatc
     1621 gaccagcggg ccgggctggc cggtctcggg ccgctcgcgc gccaccagcc gcccgatccc
     1681 ccccgccgac ttggcgaagc agaggtcgac cgaggacgcc ttgtccgcca cgtgcagccc
     1741 ggccgccgcc gcccgctcgc gcacggcgtc cttatccagc gcgcccagcg ggaagtggag
     1801 ccgggcgagt tcctcggagc ccagccggta gagcgcgtac gactggtcgc ggcccggctc
     1861 gcgcccctcg gccaggaagc ggcgcccgtc gtgctccagg atgcgcacgt agtggccggt
     1921 ggcgaccgcc tcgatgccca ggctgtcggc caggcgcagc agccccggga tgcgcacgcg
     1981 gtggttgcag gccaggcagg ggttgggtgt ctcggcgcgg gcgtaggacg cgacggtgaa
     2041 ggcggccacc tcgcgggcga agtcgtcgcg gagatcacgc acgtagaagg ggatgtcgag
     2101 ctgggcggcc acgcggcgcg cgtcggcggc ggcgaggtcg ccgcagcagg cgccgtcggc
     2161 ctcgacgtcg gcgtcgtaga ggcgcaggtg cacgccgacc ggctcccagc cggcctcctt
     2221 gagcagcgat gccgcgacgg cgctatccac gccgccggag agcgccacga tgatgcgcgc
     2281 catggtgcga gccccattcg ctgaaccagc gtcgcggttg cccggcccgg cgccggccac
     2341 cagccgcaac accccattgt atcgcgggcg gcccactcgc tgccgcttga tcgcacgtac
     2401 gacggctttt tagaattcgc tggacaagag agagtggcta tcgaagatgt tgagtgccgg
     2461 gtggagacgg atccttcgac tgtgctcagg gcaggctctg agcgcggtcg aaggatccgt
     2521 cctcgcgcgt cgcgatgcgt attcgatggc cagcagacga ggaggagcgg ccgatggtgt
     2581 atctgagcga gcaggtggtg agcctggagc tggcgcagcg gctgaaggcg ctgggcgtgg
     2641 agcaagagag tatgtactgg tggcaggagg acgggccggg gaccggccac tgggcgatcc
     2701 tgcagttccc ggccagcacc ttcctgagcc ccgcaggtcc ggtcgagctg gacaagaccg
     2761 ccgccttcaa cgtgtccgag ctgggccggc tctggcccga gctggcgcgc tcctatcgtc
     2821 gcgtcgacgg ccagtggcag gcggaggcgc cgctggagct ggccgaggcg cgcggctggg
     2881 gctgggatga gccgcggcac gcggcgggcg cgacggaggc cgacgcccgc gccgcgctgc
     2941 tgatccagct ca
//
LOCUS       DASUBI010000407         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_995722, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000407 DASUBI010000000
VERSION     DASUBI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995722"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFU72_07840"
     CDS             complement(<1..390)
                     /locus_tag="VFU72_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5423432.1"
                     /translation="MRRSEDMTFSSIDPASGERWQEHPAWGAARLEEALAAASAAVPA
                     WSARAIQERCGLLRRAGETLMRHRDEYALLMSREMGKVLAEARAEIEKSARACLYYVD
                     EAPRMLADEVIGSEAHRSLVAYQPLGLV"
     gene            450..2468
                     /locus_tag="VFU72_07845"
     CDS             450..2468
                     /locus_tag="VFU72_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5423433.1"
                     /translation="MTGPRLLLLCTALTTLCALGQNADDFTEQRAEFKLAWSYAVKGD
                     ARDLAPYLKSLKDYPLRPYLDYAYLDATLDQQPDAAVEQFLAAHPDMPVDDALRDDWL
                     VVLAERQEWDKVLAYYRDETDPALRCAAVSAHLLKEDEPDRDSWVVAARHLWLTPAIA
                     LDGKVRPAANPASSLTPPMSAACAPLFQYLDAHRLIDADMLRRRAELALQAKDYDTAR
                     ALLPRLPAYDRDWVQDWLDMAADPAVWLAHAQAPDDEAHQEMLLAGIKIVARSQPLKA
                     QHLWSTLSKQHHFSADDARAMRTLLVLQHVWHMLPDARSQLARLHDATDPQVPEWRAR
                     LALRDQDWRGVLKALPGLGADQDSPEWRYWKARALEGLGHRGASDALYLSLVHAPDYY
                     GFLSSDRLHEGYRIVQQVSKPEEEVIEQLGERPDFVRARELVAAGLYPEAEAEWAAAT
                     RGLSLPARCQSALLAERWGWHARVIPTLSGGGCWQDLALTYPLAFEQTLVPKAAQLKL
                     DLSWIYGLIRAESVFRPNAVSHAGAYGLMQLMPATGRDMANRLGLTLDDDDSLLDPPT
                     NLILGSSYLREVLRQFDGSEPLATAAYNAGVARVSDWLPQDGDMPADAWIDSIPYTET
                     RNYVHRVMGHTVMFDWRISGKPERLSVRIGRVTAPASTDSDAADVDIR"
     gene            2473..>3140
                     /locus_tag="VFU72_07850"
     CDS             2473..>3140
                     /locus_tag="VFU72_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEU5423434.1"
                     /translation="MSRVTLLGGTGFVGRHLAARLVARGHSVQVLTRHRFRHTDMLVL
                     PTLTLTQADVYDAGVLREAFLGSDAVINLVGILNERGHKGAGFREAHVTLTEAVIEAC
                     RGAGVRRLLHMSALNADKQGPSHYLRTKGEAATAVLMAKQLDATVFEPSVIFGPGDSF
                     LTRFAALLKRLPFMPLAFPKACFAPVYVGDVAAAFTHCLERREGVGRRYALCGPRVYS
                     LRELV"
BASE COUNT          460 a         1111 c         1116 g          453 t
ORIGIN      
        1 cacgaggccg agcggctggt aggccacgag gctcctgtgc gcctccgacc cgatgacttc
       61 gtccgccagc atccgcgggg cttcgtcgac gtagtagagg caggcccggg cggacttctc
      121 gatctcggcc cgggcctcgg ccaggacctt gcccatctcg cggctcatga gcagcgcata
      181 ttcatcccgg tgccgcatga gcgtctcgcc agcgcggcgc agcaggccgc agcgctcttg
      241 gatggcacgg gcgctccagg cggggacggc ggcgctcgcg gccgcgaggg cctcttccag
      301 gcgcgccgct ccccaggccg gatgctcctg ccagcgctcg ccgctggcgg gatcgatgct
      361 ggaaaatgtc atgtcctcgc tcctacgcaa cgcgcgtttc gtccacgaat catagccccg
      421 ccctcccgcg ccacggtaat ctattggaca tgacggggcc gcgtcttctc ctgctgtgca
      481 ctgcgctcac aaccctgtgt gcgctggggc agaacgcgga cgatttcacc gagcagcggg
      541 ccgagttcaa gctcgcttgg agctacgcgg tgaagggcga cgcgcgcgac ctcgcgccgt
      601 atctgaagtc gctcaaggat tacccgctcc ggccttatct cgactacgcc tacctcgacg
      661 cgaccctcga ccagcagccg gacgcggcgg tggagcagtt cctggcggcg cacccggaca
      721 tgccggtgga cgacgcgctg cgcgacgact ggctggtggt gctggcggag cggcaggaat
      781 gggacaaggt gctcgcctat taccgggacg agaccgatcc cgcgctgcgc tgcgcggcgg
      841 tgagcgcgca tctgctgaag gaagacgaac cggaccggga cagctgggtg gtggcggcgc
      901 gccacctgtg gctgacgccg gccatcgcgc tggacggcaa ggtgcgcccg gccgcgaacc
      961 cggccagcag cctgacgccg cccatgagcg ccgcctgcgc gccgctcttc caatacctcg
     1021 atgcgcaccg gctcatcgac gcggacatgc tgcgccggcg cgcggagctg gcgctgcagg
     1081 ccaaggacta cgacacggcc cgcgcgctgc tgccgaggct cccggcctac gaccgggact
     1141 gggtgcagga ctggctcgac atggcggcgg atccggcggt gtggctcgcc cacgcgcagg
     1201 cgcccgacga cgaggcccac caggagatgc tgctggcggg catcaagatc gtggcccgct
     1261 cgcagcccct caaggcgcaa cacctgtgga gcacgctctc gaagcaacac catttctcgg
     1321 cggacgacgc ccgcgccatg cgcacgctgc tggtgctgca gcacgtgtgg cacatgctgc
     1381 cggacgcgcg cagccagttg gcgcgcctgc acgacgccac cgacccgcag gtgccggagt
     1441 ggcgcgcgcg cctggcgctg cgcgaccagg actggcgcgg ggtgctgaag gcgctgccgg
     1501 gcctgggcgc ggaccaggat tcgccggagt ggcgctactg gaaggcgcgg gcgctggagg
     1561 gcctgggaca tcgtggcgcc tcggacgcgc tctatctctc gctggtgcat gcgccggact
     1621 actacggctt cctctcctcg gaccggttgc acgagggtta ccggatcgtg cagcaggtga
     1681 gcaaacccga ggaggaggtg atcgagcagc tgggggagcg cccggacttc gtgcgcgccc
     1741 gggagctcgt ggccgcgggc ctctatcccg aggcggaggc ggaatgggcg gcggccaccc
     1801 gcgggctctc gctaccggcc cgctgccagt cggcgctgct cgcggagcgc tggggctggc
     1861 atgcgcgggt gatcccgacc ctgtccggcg gcggctgctg gcaggacctc gccctcacct
     1921 acccgctcgc cttcgagcag acgctggtgc ccaaggcggc gcagctcaag ctggacctct
     1981 cctggatcta cggcctgatc cgcgccgaga gcgtgttccg ccccaacgcg gtgtcccacg
     2041 cgggcgccta cggcctgatg cagctgatgc cggccacggg ccgcgacatg gcgaaccgcc
     2101 tgggactcac cctcgacgac gacgacagcc tcttggaccc gcccaccaac ctgatcctgg
     2161 gaagctctta cctgcgcgag gtgctgcgcc agttcgacgg cagcgagccc ttggccaccg
     2221 ccgcctacaa cgcgggcgtg gcgcgggtct cggactggct gccccaggac ggagacatgc
     2281 cggccgacgc gtggatcgac agcatcccct acaccgagac ccgcaactac gtacaccggg
     2341 tgatgggcca cacggtgatg ttcgactggc gcatcagcgg caagcccgag cggctctcgg
     2401 tgcgtatcgg ccgggtgacg gccccggcga gcaccgacag cgacgcggcg gacgtggata
     2461 taagatagcc ccatgagcag ggtcacgctg ctgggtggca ccggtttcgt gggacggcac
     2521 ttggccgccc ggctcgtggc gcgcggccac agtgtgcagg tgctcacccg ccaccgcttc
     2581 cggcacaccg acatgctggt gctgcccact ctcacgctca cccaggcgga cgtctacgac
     2641 gcgggcgtgc tgcgcgaggc cttcctgggc tcggacgcgg tcatcaacct cgtggggatc
     2701 ctgaacgagc gcggccacaa gggcgcggga ttcagggagg cccacgtgac gctcacggag
     2761 gcggtgatcg aggcctgccg cggcgcgggg gtgcgccgcc tgctgcacat gagcgcgctc
     2821 aacgcggaca agcagggacc gagccattac ctccgcacca agggcgaggc ggcgacggcg
     2881 gtgctcatgg ccaagcaact ggacgcgacg gtgttcgagc ccagcgtgat cttcggcccc
     2941 ggcgacagct tcctgacgcg cttcgcggcg ctcctgaagc gcctgccttt catgccgctc
     3001 gccttcccca aggcgtgctt cgcgccggtg tacgtggggg acgtggcggc ggccttcacg
     3061 cactgcctgg agcggcgcga gggcgtcggc cggcgctacg cgctctgcgg gccgcgcgtc
     3121 tacagcctgc gggagctggt
//
LOCUS       DASUBI010000408         5859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_864466, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000408 DASUBI010000000
VERSION     DASUBI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864466"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VFU72_07855"
     CDS             complement(<1..1264)
                     /locus_tag="VFU72_07855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5423435.1"
                     /translation="MATVIMPKMGDAMEEGTLLRWLKGEGEEVKQGEPIAEIETDKVN
                     LEIESNDAGVMRKHLVDEGATVPIGEPIAVIAAPGEEVEIPAPASQPQPVAAQPAARP
                     AAALAGQAPAESAAPGRINAEQAPEAPSNNREASVAAAMPEQVLERAPGERVRASPLV
                     RRLAAEHGIDLNQVRGTGPGGRVVKVDVMPLIGRPATQPAPATAPPAPARSPQPAPAP
                     AAVAAAAPGVELRELPRIRRTIAKRMSESWQQAPHFFVTMVIGMDKAMALREQINGEI
                     EDTASQVSVNDLILKASALALRAFPKFNASFAGDQLQMHQRIDVAFAVAFEGGLISPV
                     IPETDRTSLGEIARMTKDLSRRVREGTIRPEEFQGGTFTVSNLGMFGVDDFTAIINPP
                     QAAILAVGTITIEPVYQDGQFKPAQQMKV"
     gene            complement(1273..2259)
                     /locus_tag="VFU72_07860"
     CDS             complement(1273..2259)
                     /locus_tag="VFU72_07860"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5423436.1"
                     /translation="MPVMTYRDALRSAMAEEMERDERVVLLGEDIGLYGGTHLVTNGL
                     LEQFGPKRVVDTPISEAGFTGAAIGMAMLGMRPIVEMMTWNFSYLATDQIVQNAAKVH
                     YFSAGQVSVPLVIRGPNGGGVQLSAQHTHSVEGFYAQFPGLKVVSPVTPHDAKGMMLE
                     AIRDPNPIIFLEAGALYGTKGEVPEGDYTVPFGQARVARQGSDVTLISYGRSVNLLLQ
                     AAETLEKEDDVQAEVIDLRSIRPFDEATIVESFKKTNRAVVVQEQWRWFSVASEVAAI
                     IQEQAFDDLDAPVERVSGAEAPAPYARNLEIAAFPSEQDVLNAARRALYRKG"
     gene            complement(2296..3321)
                     /locus_tag="VFU72_07865"
     CDS             complement(2296..3321)
                     /locus_tag="VFU72_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5423437.1"
                     /translation="MTSSGGSQTKDRVEEQSHLLDLYRQMVLVRLFEDAAQLGFRTGK
                     IGGYLHVYSGQEAIATGFLDAFQPGDRVITAYRDHAHALLLGSTPREVMAELYGKATG
                     LVKGKGGSMHLFNVDKGLMGGWGIVGGHIPVGVGLAYALAYQGNGGITQLYFGDGAIH
                     EGAFHEAANLSGLWGRDGMNPCLFVLENNHYGMGTSVERATAMTDLGAKFSSYGIEHE
                     KVDGNDLEAVLAVAHRAVRRVRESGAPYAVEAITYRLEAHGAADFLQKYRSKEEVAEH
                     RKRDPIGLLEQRLLEIGAASEGDMADIRAENKAIIDDAVQFAEESPEPPLEELYENVY
                     AQPEPEE"
     gene            complement(3527..4030)
                     /locus_tag="VFU72_07870"
     CDS             complement(3527..4030)
                     /locus_tag="VFU72_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEU5423438.1"
                     /translation="MSVELVSYAIQNPTGLNLIFGQSHFIKTVEDLYEALAGSVPGLK
                     FGLAFSEASGDRLVRRAGSDDELVELATRNVLEIAAGHCFLILLREAFPINVLPAVKA
                     VQEVCTIFCATANPVRVILADVGDGRAVLGVVDGFKPLGVESRQQVVEREALLRRFGY
                     KLGGGPG"
     gene            4138..5574
                     /gene="pyk"
                     /locus_tag="VFU72_07875"
     CDS             4138..5574
                     /gene="pyk"
                     /locus_tag="VFU72_07875"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5423439.1"
                     /translation="MSVADRSHRRHTKIVATIGPATWDLATLQEVIRAGANVLRINAA
                     HNDIETRWRIVRTARQAARETGCRIALLQDLAGLKPRTGPLPSGQTVALERGQTIELV
                     AGDEPLRPGRITIEDAPLVAQLAPGQRVLMADGLLEVVVEERRGETALARVVRGGRLR
                     GRQGVTVPGAPAPPRVITPQEAADIAFAAEAELEYLGVSFVTQGQDIEQVRRELRRHG
                     GHAGIVAKIERPEALAAIHEIAHESDALMVARGDLGVQLPPEEVPFAQKQIIDVARRH
                     GVPVITATQMLESMTTQPIPTRAETSDVANAVLDGTDAVMLSAETATGEYPIEAVEMM
                     DRIVCATEAHFPPRTTNSAEGPITVASTIARAATDIARRSPLVNLIAVLTRSGFSARE
                     VARERPGVPIVALTNSEFVANQLTLVWGVTSLVAPFADTTEALFQEMTERLLSARRTK
                     PGQGILFVGSLTVYHEPGHTNLLHLRQV"
BASE COUNT          850 a         2023 c         2046 g          940 t
ORIGIN      
        1 tcaccttcat ctgctgcgcg ggcttgaact gcccgtcctg atagaccggc tcgatcgtga
       61 tcgtacccac cgccaggatc gccgcctgcg gcgggttgat gatggcggtg aagtcgtcga
      121 cgccgaacat gcccaggttg ctgacggtga aggtgccgcc ctggaactcc tcggggcgga
      181 tcgtgccctc gcgcacgcgc cgcgacagat ccttggtcat gcgcgcgatc tcaccgagcg
      241 aggtgcggtc ggtctcgggg atgaccggac tgatcagccc gccctcgaag gctacggcga
      301 aggccacgtc aatgcgctgg tgcatctgca gctgatcccc ggcgaagctg gcgttgaact
      361 tcgggaacgc gcgcaacgcc agggcggacg ctttcaggat caggtcgttg accgagacct
      421 gagacgccgt gtcctcgatc tcgccgttga tctgctcgcg cagcgccatc gccttgtcca
      481 tgccgatgac catggtgacg aagaagtggg gcgcctgctg ccagctctcg ctcatgcgct
      541 tggcgatggt gcggcggatt cgcggcagct cgcgcagctc gacgccgggc gcggcggccg
      601 ccacggccgc gggagccgga gcgggctgcg ggctgcgggc tggcgcgggc ggcgcggtcg
      661 cgggagcggg ctgcgtcgcc gggcgcccga tcaggggcat gacgtcgacc ttgactacgc
      721 gcccgccggg accggtgccg cgcacctgat tcagatcgat gccgtgctcg gccgccagcc
      781 gccgcaccag cggcgaggcg cgcacgcgct cgcccggcgc gcgctccagc acctgctcgg
      841 gcatggccgc ggccacgctg gcctcgcggt tgttgctcgg cgcctcgggc gcctgctcgg
      901 cgttgatccg ccccggcgcg gcgctctcgg cgggggcctg gcccgccagg gcggcggctg
      961 gccgcgcggc cggctgggcc gcgaccggct gcggctggct ggcgggggcg gggatctcga
     1021 cctcctcgcc cggcgcggcg atgaccgcga tcggttcgcc gatcggcacg gtggcgccct
     1081 cgtcgaccag gtgcttgcgc atcacgccgg cgtcgttcga ctcgatctcc aggttgacct
     1141 tgtcggtctc gatctcggcg atcggctcgc cctgcttgac ctcctcgccc tcgcccttca
     1201 gccagcggag gagcgtcccc tcctccatgg cgtcgcccat cttgggcatg atcacggtcg
     1261 ccatgctacc ccctacccct ttcggtacaa cgcgcggcgc gccgcgttga ggacgtcctg
     1321 ctcgctgggg aaggcggcga tttcgaggtt gcgagcgtag ggcgccggcg cctccgcgcc
     1381 gctgacgcgc tcgaccgggg cgtccagatc gtcgaaggcc tgctcctgga tgatggccgc
     1441 gacctcactg gccaccgaga accagcgcca ctgctcctgc accaccaccg cgcggttggt
     1501 cttcttgaac gactccacga tcgtcgcctc gtcgaagggg cggatcgagc gcaggtcgat
     1561 cacctcggcc tggacgtcgt cctccttctc cagcgtctcg gccgcttgca ggaggagatt
     1621 cacggagcgc ccgtagctga tcagggtgac gtcgcttccc tggcgggcca cgcgcgcctg
     1681 accgaagggg acggtgtagt cgccctcggg gacctcgccc ttggtgccgt agagcgcgcc
     1741 cgcctccagg aagatgatcg ggttggggtc gcgaatggcc tccagcatca tccccttggc
     1801 gtcgtgcggg gtgaccggcg aaacgacctt gaggccgggg aactgggcgt agaagccctc
     1861 gacgctgtgg gtgtgctggg ccgagagctg cacgccgccg ccgttcgggc cgcggatcac
     1921 cagcggcacc gagacctgcc ccgccgagaa gtagtgcact ttggcggcgt tctgcacgat
     1981 ctggtcggtg gccaggtagg agaagttcca ggtcatcatc tcgacgatcg ggcgcatgcc
     2041 gagcatggcc atgccgatgg ctgcgccggt gaagccggcc tcgctgatcg gcgtatcgac
     2101 cacccgcttg gggccgaact gctccagcag cccgttggtg acgagatggg tcccgccgta
     2161 gaggccgatg tcttcgccga gcagaaccac gcgctcgtcg cgctccatct cctcggccat
     2221 cgcgctgcgt agcgcgtcac gatacgtcat cacgggcatg gcccgctcct tttctcctcc
     2281 ggtgcgggcg tgccgctact cttccggctc aggctgggcg tacacgttct cgtagagctc
     2341 ctcgagcggc ggctcggggc tttcttcggc gaactggacg gcgtcgtcga taatcgcctt
     2401 gttctcggcc cggatatccg ccatgtcccc ttcgctggcc gcgccgatct ccagcagccg
     2461 ctgctccagc aggccgatcg ggtcgcgctt gcggtgctcg gcgacctcct ccttgctgcg
     2521 gtacttctgc aggaagtcgg cggcgccgtg cgcctccagg cggtaggtga tggcctccac
     2581 cgcgtagggc gcgccgctct cgcgcacacg tcgcaccgcg cggtgggcaa ccgcgagcac
     2641 cgcctccagg tcattgccgt cgaccttctc gtgctcgatg ccgtaggagc tgaacttggc
     2701 gccgagatcg gtcatggcgg tggcgcgctc gacgctggtg cccatgccgt agtggttgtt
     2761 ctccagcacg aagaggcagg ggttcatgcc gtcgcggccc cagaggccgg agaggttggc
     2821 tgcctcgtgg aaggcgccct cgtggatggc gccgtcgccg aagtagagct gggtgatgcc
     2881 gccgttgccc tggtaggcca gcgcgtaggc caggccgacg ccgaccggga tatgaccgcc
     2941 gacgataccc cagccgccca tcagcccctt gtcgacgttg aagagatgca ttgagccgcc
     3001 cttgcccttg accagcccgg tggccttgcc gtagagctcg gccatgacct cgcgcggggt
     3061 gctgccgagg agcagcgcgt gggcgtggtc gcgataggcg gtgatgaccc ggtcgccggg
     3121 ctggaaggcg tcgagaaagc cggtggcgat cgcctcctga ccgctgtaga cgtggaggta
     3181 gccgccgatc ttgcccgtgc ggaaaccgag ctgcgcggcg tcctcgaaca ggcggaccag
     3241 caccatctgt cgatagagat caagaagatg gctttgctcc tctacgcgat ccttagtctg
     3301 actacctccc gaactggtca tgcccttagc ctccagattc gactgcgacg cgctggagcg
     3361 gcgcccggtg gtcgcgggct acgtctcgag atcggatcga cacccaatgc agacccctgc
     3421 cacgcttcca cgctcgggca cgccaaggcg tgatgcgtgg caccccagcc tctgattgta
     3481 ccgaagtcgt acgacccatt ccagcgagag gcatggggcc gcgcaattag ccgggcccgc
     3541 caccgagctt gtatccgaaa cgccgcagca gcgcctcgcg ctcgaccacc tgctgccggc
     3601 tctcgacgcc gagtggtttg aagccgtcga ccacgcctag cacggcacgc ccgtcgccga
     3661 cgtcggccag gatgacccgc accgggttgg cggtggcgca gaagatggta cagacctcct
     3721 gcacggcctt gaccgccggc agcacgttga tcgggaaggc ctcgcgcagc aggatcagga
     3781 agcagtggcc cgcggcgatc tccagcacgt tgcgggtggc cagttcgacc agctcatcgt
     3841 cgctgccggc gcggcgcacc aggcggtcgc cggaggcctc gctgaaggcc aggccgaact
     3901 tgagacccgg caccgagccg gccagcgcct cgtagaggtc ctcgaccgtc ttgatgaagt
     3961 ggctctggcc gaagatcagg ttgagcccgg tagggttctg gatggcatag ctgaccagct
     4021 cgaccgacat cgggacctcc ttcccacgac gacggggcgc cgtcaatggg ggcgcaccgc
     4081 tctcctatgg tatcgtactc cgcgcgaagt accgtcttga atagagagtt gagggctgtg
     4141 agcgtagccg accgttctca tcgccgccac accaagatcg tcgcgaccat tgggcccgcc
     4201 acctgggacc tcgccacgct gcaagaggtg atccgcgccg gcgccaacgt gttgcggatc
     4261 aacgccgccc acaacgacat cgagacacgc tggcgcatcg tgcgcaccgc ccgccaggcg
     4321 gcgcgcgaga ccggctgccg gatcgcgctc ctgcaagacc tggccgggct caagccgcgc
     4381 accggcccgc tgcccagcgg ccagacggta gcgctggagc gggggcagac gatcgagctg
     4441 gtggccggcg acgagccgct gcggccgggg cggatcacca tcgaggacgc gccgctggta
     4501 gcgcagctcg cgccggggca gcgggtgcta atggccgacg ggctgctgga ggtggtggtc
     4561 gaggagcggc gcggcgagac ggctctggcg cgggtggtgc gcggcgggcg gctgcgcggg
     4621 cgccaggggg tgacggtgcc gggcgcgccg gcaccgccac gcgtgatcac gccgcaggag
     4681 gcggccgaca tcgccttcgc cgccgaggcc gagctcgagt atttgggcgt cagcttcgtc
     4741 acccagggcc aggacatcga gcaggtgcgc cgggagctgc gccgccacgg cgggcacgcc
     4801 ggcatcgtgg ccaagatcga gcgccccgag gcgctggcgg cgatccacga gatcgcgcac
     4861 gagtcggacg cgctgatggt ggcgcgcggc gacctgggcg tgcagctccc gcccgaggag
     4921 gtgcccttcg cccagaagca gatcatcgac gtggcgcgcc ggcacggggt gccggtcatc
     4981 accgccacgc agatgctgga gtcgatgacc acccagccga tcccgacgcg ggccgagacg
     5041 agcgacgtgg ccaacgcggt gctcgacggt accgacgcgg tcatgctcag cgccgagacc
     5101 gccactgggg agtacccgat cgaggcggtc gagatgatgg accggatcgt ctgcgccacc
     5161 gaggcgcatt tcccgccgcg cacgacgaac tccgccgagg ggccgatcac ggtcgcctcg
     5221 accatcgccc gcgcggcgac cgacatcgcc cgccgctcgc cgctggtcaa cctgatcgcg
     5281 gtgctgacca gaagcggctt ctccgcgcgc gaggtggcgc gcgagcgccc gggcgtaccg
     5341 atcgtggcgc tgaccaacag cgagttcgta gccaaccaac tgacgctcgt ctggggcgtc
     5401 acctcgctgg tggcgccctt cgccgacacc accgaggcgc ttttccagga gatgaccgag
     5461 cgactgctct cggcccggcg caccaagccg ggccagggca ttctcttcgt cggcagcctc
     5521 accgtctacc acgagccggg ccacaccaac ctgctgcatc tgcgccaggt gtagcgcggt
     5581 cgcccggccg tgaggctccc gtggcccgtc agcacggatc cctccactgc ggtcgggatg
     5641 acaagtgtag ttaaggggcg gacgaggagg ttgggtcttt gacgatgaca tatctcgtcc
     5701 ggcaggccgc atccctttcc ctgtcatcct gagtggttcg accgtcatga tttgtccggt
     5761 tcgaggaccg gatagagccg cttgagtttg atgcgggcat cggcgggggt gaactgccaa
     5821 tcgatggtga ccgccgcggc gttgcgggcc tgcacccag
//
LOCUS       DASUBI010000409         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_628832, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000409 DASUBI010000000
VERSION     DASUBI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628832"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFU72_07880"
     CDS             <1..508
                     /locus_tag="VFU72_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423440.1"
                     /translation="LRRAGWRVVRLERPMVSHYTAPADRIASIFGRRRRRLYLGAGQV
                     LRRHARTPLARQYLRERGFALPAAAGLGATGLAVAVSLGRRDIRPLSLWLVVVALVLA
                     GDAARKRSLYRTVHSLMVRLLFLEGTVRGYRHGTDGRELPRYDVLRATTDDMLVPSNL
                     DSLKGEVA"
     gene            505..1518
                     /locus_tag="VFU72_07885"
     CDS             505..1518
                     /locus_tag="VFU72_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5423441.1"
                     /translation="MRVLVVTNMYPTPEKPVFGTFVRTQVECLRRAGIDVDVLAMDGR
                     FRKLIYPRGIVQLQQRLARERYDLVHAFYGYSGMVARCQWKLPLVVTYEGSDLNGATG
                     WGGRRGRSNAVVVPAGKLLARLADAAIVQSQEMADRIPNANVHVVPHEVDFDTFQVTP
                     RDEARRQLGLSLDKKYLLFGANPEIGRKNFALARAAAAQVAADDPSVELLVVWRESQP
                     RLALYMSACDALVFPSYSEGSPNVVKQALACNLPIVATDVGDIAEVIAGTDLCFLADF
                     SADEFAARLRQILATRPRTNGRRQAQRYAPEIIVGKVRAIYDDVLSRQSRGLARAALD
                     RGE"
     gene            1601..>2249
                     /locus_tag="VFU72_07890"
     CDS             1601..>2249
                     /locus_tag="VFU72_07890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5423442.1"
                     /translation="MRDAPGNRVLMLLENQPYPRDERVRREALALAEAGYRVTVICHA
                     DQGQPRSEELNGVRVLRYPGFFPGSGALGFVWEFGYATLATLALALWVALRHGVDVVH
                     SHNPPDTFFVIGALFKLGGKRFVFDHHDLAPDMYRAMFGGHASGLLFRILCLLEHASC
                     RVADHVIATNQSYRAVEIERDHVPPERVTVVRNGPDINRMGPVTPDPALRARAGTI"
BASE COUNT          343 a          740 c          791 g          375 t
ORIGIN      
        1 gctgcgccga gccggctggc gcgtggtccg cctggagcgc ccaatggtca gccactacac
       61 ggcgccggcc gaccgcatcg cgtcgatatt cgggcgccgg cgtcgccgcc tctatctggg
      121 cgcgggccag gtgctccggc gccacgccag gacgccgctc gcacggcagt acctccgtga
      181 gcgcggcttc gccttgccgg cggcggccgg actgggagca accgggctgg cggtagcggt
      241 gagcctggga cggcgggata tccgcccgct ctcgttgtgg ctggtcgtcg tcgcgctcgt
      301 gctggcgggc gacgccgcgc gcaagcggag cctctaccgc acggtgcata gcctgatggt
      361 gcgcctcctc ttcctcgaag gaaccgttcg tggctaccga cacgggaccg atggccggga
      421 gttgccacgc tatgacgtgc tgcgggcaac aactgacgac atgttggttc cgtccaacct
      481 ggattctttg aagggagaag tcgcgtgaga gtgctggtgg tgacgaacat gtatcccacg
      541 ccggaaaagc cggtgttcgg gaccttcgtg cgcacacagg tggaatgcct gcgacgtgcg
      601 gggatcgacg ttgacgtgct ggccatggat gggcgcttcc gcaagctgat ctacccgcga
      661 ggcatcgtcc agcttcagca gaggctggcc cgtgagcgct acgacctggt ccatgccttc
      721 tacggctaca gtggcatggt ggcgcgttgc cagtggaagc tgccgctggt ggtgacctac
      781 gagggttccg atctgaacgg cgcgaccggc tggggcggcc gtcgcgggcg gtccaatgcc
      841 gtcgtcgtgc cggccggcaa gctgctcgct cgcctggcgg acgcggccat cgtgcagagc
      901 caggagatgg cggacaggat cccgaacgcc aatgttcacg tcgtgccgca cgaggtcgac
      961 ttcgatacgt tccaggtgac accccgcgac gaggcgcgcc ggcaactggg cctgtcgctg
     1021 gacaagaagt atctcctctt cggcgcgaac cccgagatcg gtcgcaagaa tttcgcgctg
     1081 gcccgagcag cggccgcgca agtcgccgcg gacgatccct ccgtcgaatt gctggtcgtc
     1141 tggcgcgaga gccagccgcg tctcgcgctc tacatgagcg cctgcgacgc gctggtcttc
     1201 ccctcctact ccgaaggctc acccaacgtg gtcaagcagg cactggcctg caacctgccg
     1261 atcgtggcca ccgacgtggg cgatatcgcc gaggtgatcg ccgggactga cttgtgcttc
     1321 ctggcggact tctcggcgga tgagttcgcg gcccggttgc gccagattct ggcgacacgt
     1381 ccgcgcacga acggccggcg ccaggcgcag cgctacgcgc ccgagatcat cgtggggaag
     1441 gtacgggcca tctacgacga cgtgctgtcg cgccagtcgc gcgggctggc gcgggccgcg
     1501 ctcgaccgag gcgaatgatg cgcggcatcc ctggtgatga cgatggtgct gctcgatgcg
     1561 cgtgcgcgga gcgtgccgtc agaggcgaga ggagcggacg atgcgagatg caccgggcaa
     1621 cagagtcttg atgttgctgg agaaccagcc gtatcccaga gacgagcgcg tgcggcgcga
     1681 ggcgctggcg ttggccgagg caggctaccg cgtgaccgtc atctgccacg ctgaccaggg
     1741 gcagccacgc agcgaggagc tcaacggcgt gcgcgtgctg cgctaccccg gtttcttccc
     1801 cggctcaggc gcgctgggat tcgtctggga attcggctac gccacgctgg cgacgctggc
     1861 actggcgctc tgggtggcgc tgcgtcacgg ggtcgacgtg gtccacagcc acaatccgcc
     1921 ggacaccttc ttcgtgatcg gggcgctctt caaactgggc ggcaagcgct tcgtcttcga
     1981 tcaccacgac ctggcgccgg acatgtaccg cgcgatgttc ggcgggcatg ccagcgggtt
     2041 gctcttccgg atcctctgcc tgctggagca cgcctcgtgc cgggtcgcgg atcacgtcat
     2101 cgcaaccaac cagtcgtacc gggcagtcga gatcgagcgc gatcacgtgc cgcccgagcg
     2161 cgtcacggtc gtgcgcaacg gaccggacat caaccggatg ggaccggtca cgcccgatcc
     2221 cgcgctgcgc gcgcgcgccg gaacgattc
//
LOCUS       DASUBI010000410         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_972322, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000410 DASUBI010000000
VERSION     DASUBI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972322"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFU72_07895"
     CDS             <1..419
                     /locus_tag="VFU72_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEU5423443.1"
                     /translation="VACSADFDTADQMSWRITDHGFAMGLSRRVPVTLRRNVGGAVEH
                     LLAPHDLAPEDISHWLVHPGGPDILTAIHGAMGMCDGQLALSWQVLRERGNCSSATVL
                     LILERLLRARRARPGEWGVMMAFGPGLTLESCLLRF"
     gene            514..1353
                     /locus_tag="VFU72_07900"
     CDS             514..1353
                     /locus_tag="VFU72_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5423444.1"
                     /translation="MRRVWPYIALIHPWAVLVVMLATALFGLLAAGGRPDPARYALLL
                     AGMLGGQVAIGALNEYRDRELDARAKPWRPLPSGAVTPTAARRLVALGLLVMTGAGLL
                     LGLAPLLVLLILTGAGLAYDLWFKATRWSWLPYLVALPLLPSWVWLTLGRPAPRLLLL
                     YPLGAPMVLAVHLAQSLSDAESDRAAGAGSLVARLGRREALALLGAAALLACLEIALF
                     ALWLSARPAPALLAAVVSALLLAGALAADRLAPRLLDRYLFHLTTLLTIALGAGWVLS
                     AGL"
     gene            1833..3209
                     /locus_tag="VFU72_07905"
     CDS             1833..3209
                     /locus_tag="VFU72_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5423445.1"
                     /translation="MPRDRQPAGPPRLLLAVLVLGLALATLAGLAPSARPWLSAASAL
                     PPTPGPSLNPAAPPRIPLVAAATPHDQVTAAGPLVDPGCSHGCIARVADGGVGSGAVL
                     WQRDSVAYVAATSETFASLRDPVVTWSGLETLSLYVLSHVRPEQDALLGQYGTVRDRA
                     GDLRLLEASHVPLQVDQIYAAGIWVEKLQPDPFVGPGSVARSIPETIAEFSAINSRDG
                     VLGDREFDDPGTILAAEYLFRRYRQLGFEVRYVDFTGPSGRHQVNVVADPPHGRVEPA
                     TLITGHYDSISPPGAPAPGADDNGSGIAAMLELAARVAKQGFSPPVSFVAFGAEEPGL
                     YGSDDYVRRLASAGVKLKAVINLDAIGIPNNGNTVTIDGDDNSRWIYRDLVALSGDRF
                     WLEWLASNRYLSDDEHFRRAGYPAILVTSHIWGTEPLHHTPNDTIGHVDMNQVAGLTD
                     LIWRWLQA"
     gene            complement(3562..>4012)
                     /locus_tag="VFU72_07910"
     CDS             complement(3562..>4012)
                     /locus_tag="VFU72_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423446.1"
                     /translation="EIDQLPAGVVSLVGDHAEGEIVIDGPLSLLDELDAPIRLLIEES
                     TIVAIEGGVSAAQLRALLDAFPDPAARRAAHHCPAEVGIGTNPSVVYQPAARFSPEQA
                     RAVGMVHVGLGDDHLFPHGSVVAPLHADFLLPGAEIWLDGNRFQVEL"
BASE COUNT          531 a         1434 c         1442 g          605 t
ORIGIN      
        1 atgtcgcctg ctctgccgac ttcgacacgg cggaccagat gtcctggcgc atcaccgatc
       61 acggcttcgc catggggctc tcgcgccgcg tgccggtcac gctgcggcgc aacgtcggcg
      121 gcgcggtcga gcatctgctg gcgccgcacg acctggcgcc ggaagacatc agccactggc
      181 tggtgcatcc cggcggcccc gacatcctga ccgcgatcca cggcgccatg gggatgtgcg
      241 acggacagct ggcgctctcc tggcaggtgc tgcgcgagcg cggcaactgc tcttcggcca
      301 ccgtgctcct catcctggag cggctgctgc gcgcccgccg cgcccgcccg ggcgagtggg
      361 gcgtgatgat ggccttcggg ccggggttga cgctggagag ctgcctgctg cgcttctagc
      421 gacaccggcg accggcagcg ccgcatactc gagcacgacg gctgcgcgaa gtcctggcag
      481 cgggtggcga ctttcccgag ggagcgcgtt gaattgcgcc gcgtctggcc ctacatcgcg
      541 ctgatccacc cctgggcggt gctggtcgtg atgctggcga ccgcgctctt cgggctgctg
      601 gccgcgggcg gccggcccga cccggcgcgc tacgcgctcc tgctggccgg catgctcggc
      661 gggcaagtcg cgatcggcgc gctcaacgag taccgcgatc gggagctgga cgcgcgcgcg
      721 aagccctggc ggccgctccc ctccggcgcg gtcacgccca cggcggcgcg gcgcctggtc
      781 gcgctcggcc tgctggtgat gaccggcgcg gggctgctgc tcgggctggc gccgctgctg
      841 gtgctgctga tcctgaccgg cgccgggctg gcctacgatc tctggttcaa ggcgacgcgc
      901 tggagctggc tgccctatct ggtggcgctg ccgctcttgc cgagctgggt ctggctgacc
      961 ctcggccgcc ccgcgccgcg cctgctgctc ctctacccgc tgggcgcgcc gatggtgctg
     1021 gcggtgcatc tggcgcagtc gctgagcgac gccgagagcg accgcgcggc gggcgcgggc
     1081 tcactggtgg cccggctcgg ccggcgggag gcgctggccc tgctgggggc ggcggcgctg
     1141 ctggcctgcc tggagatcgc cctcttcgcg ctctggctga gcgcgcgccc agcgccggcg
     1201 ctgctggccg cggtggtcag cgcgctgctg ctggcgggcg cgctggcggc tgaccggctc
     1261 gccccgcgcc tgctcgaccg ctacctcttc cacctgacga cgctgttgac gatcgcgctc
     1321 ggcgccggct gggtcctctc ggcggggttg tgacaggcgg tggccagtga cgacattgat
     1381 tgccgctcca cgacggatcc ttcgcctgcg ctcagagcct gccctgagcc tgccgaaggg
     1441 atgacaacgg gaggggatgg ggcgtacgac gacgcgcatc ctcgccggtg cagaccctcc
     1501 gcgtcagccc cttattcacc ctgtcatccc gaccgcagtg gagggatccg tcctcgctgg
     1561 caacgaaggt tgtcgttggg cggtgaacgg tagccaccga tggcgttcag cggcgttcca
     1621 ccttggattc ctcgacagat tcggggtgac aggggaaggg aggggcgacc ggcgacgata
     1681 tgccggccca tccgccagac gctccactcc gtggtgggaa agggccggaa ggtgcggccg
     1741 cgcgagatgc cggtagaatg tggcgtgccg ccgctgcgaa gcagcatgag acacgcggcc
     1801 catgggtggt ctgagaggga cagggcggga gtatgccacg agatcgacag ccagccggcc
     1861 cgccgcggct gctgctcgcc gtgctggtgc tcgggttggc gctggcgacg ctggccgggc
     1921 tggcgccgag cgccaggccc tggctgtcgg ccgcgagcgc gctcccgccg acgcccggtc
     1981 cgtcgttgaa cccggccgcc ccaccgcgca tcccgctcgt cgccgctgcc acgccgcacg
     2041 atcaagtgac cgcagccggg ccgctggtcg atcccggctg ctcccacggc tgcatcgcac
     2101 gcgtggcgga tggcggcgtc ggctccggcg cggtcctctg gcagcgggac agcgtcgcct
     2161 acgtggccgc cacttccgag accttcgcct cgctgcgtga cccggtggtc acctggtccg
     2221 ggctggagac gctctcgctc tacgtcctct cgcacgtgcg ccccgagcag gatgcgctgc
     2281 tcggccagta cgggaccgta cgcgaccgcg ccggcgatct gcggctgctg gaggccagcc
     2341 acgtgccgct ccaggtggac cagatctacg ccgccgggat ctgggtcgag aagctccagc
     2401 ccgacccgtt cgtcggaccc ggcagcgtgg cgcgctcgat ccccgagacg atcgccgagt
     2461 tctccgcgat caacagccgc gacggggtgc tgggcgaccg cgagttcgac gacccgggca
     2521 cgatcctggc cgccgagtac ctgtttcgcc gctatcgcca gctcggcttc gaggtgcgct
     2581 acgtcgactt caccggcccc agcggccggc atcaggtcaa cgtggtggcc gatccaccgc
     2641 acgggcgtgt cgagccggcc accctgatca ccggccacta cgactcgatc tcaccgccgg
     2701 gggcgccggc gccgggcgcc gacgacaacg gcagcggcat cgccgccatg ctggagctgg
     2761 cggcgcgggt cgccaagcag ggcttctcgc cgccggtcag cttcgtcgcc ttcggcgccg
     2821 aggagccggg gctctacggc agcgacgact acgtgcgtcg gctggccagc gctggcgtca
     2881 agctcaaggc ggtcatcaac ctcgacgcca tcggcatccc caacaacggc aacaccgtca
     2941 ccatcgacgg cgacgacaac tctcgctgga tctaccgcga cctggtggcg ctcagcggcg
     3001 accgcttctg gctggagtgg ctggccagca accgctacct cagcgacgac gagcacttcc
     3061 gccgcgccgg ctaccccgcg atcctggtga cgagccacat atggggcacc gagccgctgc
     3121 accacacgcc gaacgacacc atcgggcacg tcgacatgaa ccaggtcgcc ggcctgaccg
     3181 acctcatctg gcgctggctg caggcgtgac ccggtgggtc tgggccgtgg tggtgagacc
     3241 gctgtcctgg gaacgccggc agcgcggcgg cggttcagtg cccatcgacg acgttgaggg
     3301 ccgggtgagg acggatcctt cgactgcgct cagagcctgc cctgagcctg ccgaagggat
     3361 gacagggtaa aggggatgcc ctaccggact gggcaagcct tcgtcgtctg acgccgaccc
     3421 tcctcgtcag cccggccatt caccctgtca tcccgaccgc agtggaggga tccgtcctcg
     3481 ctggcaccga aagttgtcga tgggcagcgc ctgtcgaagg cagcggagat ccttatgatc
     3541 gggaagcggc agtcgtgacc ctcacagctc gacctggaag cggttgccgt cgagccagat
     3601 ctcggcgccc ggcagcagga agtcggcgtg gagcggcgcc accaccgagc cgtgggggaa
     3661 gaggtggtcg tcgccgaggc cgacgtggac catcccgacc gctcgcgcct gctccggact
     3721 gaagcgcgcg gccggctggt agacgaccga ggggttggtg ccgatgccga cctcggccgg
     3781 gcaatgatgg gccgcccggc gcgcggcggg gtcggggaag gcgtcgagca gcgcgcgcag
     3841 ttgggcggcc gagacaccgc cctcgatcgc cacgatggtg ctctcctcga tcagcagccg
     3901 gatcggcgcg tccagctcat ccagcagact gagcgggccg tcgatcacga tctcgccctc
     3961 ggcgtggtcg ccgaccaggc tgaccacgcc cgccggaagc tggtcgatct cg
//
LOCUS       DASUBI010000411         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_658496, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000411 DASUBI010000000
VERSION     DASUBI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658496"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFU72_07915"
     CDS             complement(<1..742)
                     /locus_tag="VFU72_07915"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEU5423447.1"
                     /translation="MSDDELLVSDAERESALSGLREAYVEGRLNLEEYSQRMDAALAA
                     RTRGQLAELTRDLPATVAAAVPAAPATPRGPLQPAPDWTVSIMSDTKRQGRWRLGAPL
                     NALSLMSSLRLDLRQSEIPHDSVLNVYVIMGDVKILVPEGVEVAFEEITLMGSQQDKR
                     RSPPPPPGAPVVRLRGFVLMGNVEVLNGEQTFMGKMAERFGGRVSPPVPPGELGPPSR
                     PLTRHEERLERHIERRLDQLERRIERHDR"
     gene            complement(842..2608)
                     /locus_tag="VFU72_07920"
     CDS             complement(842..2608)
                     /locus_tag="VFU72_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5423448.1"
                     /translation="MALDDVLREVSVENAWRHIEHITGQIPSRLAGSENSRRMAEYAY
                     HVFQEAGLDSSMHHFPGLVSFPEPAEVRVLAPETYTIEANTLGHSASTPGIEGELVYV
                     GSGAEADYEGKDVRGKITLSELSYSPARHEKAYIAWKRGSSAQIMMNWGAPDNPAVPF
                     GSMKSAWGNPTPATLEQEMPDIPCVGIARTEGLRLKELCQRGTVRVWLNAQADNGWQP
                     LTMTSAEFGAARGRQFLLLGGHMDSWPGPQATDNAAGDACIMELARVFSQHQDELRRG
                     IVAGLWMGHETGTMISSSRFADVNWDRLRHACVAYLQIDQPAITGSSVWHVHSTDDIQ
                     RYAVAAAKEMVGEMPVHWRRQQKNGDSSFFGVGLASLAGEMTYTDEEIQRTALATLGW
                     WHHSIHNTLDKLDKGKLATHLRVYANWVWGLLTAPILPHEYEPLGRRFVERLSDLAAL
                     DVPGIDMAGAVERAKEFHGLAARLDETSRAWQARLAGDGGEGGQQAADALNHAKLWLS
                     RTLVPVASTAAGPYGQDRYGHAWQTQMIPALVPYPALATYARDSEEYQTWWVAMIRAR
                     NQVSDALEQAGEIARRALAEVG"
     gene            2779..3246
                     /locus_tag="VFU72_07925"
     CDS             2779..3246
                     /locus_tag="VFU72_07925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423449.1"
                     /translation="MNEDQTIRLARPDEWGAVLSCVEDAYAKYVPRMGMKPAPMLADY
                     PALIARDEVYVVPGPEGVRGVLVIEPRADHLFIENIAVRPDEQGRGYGFQLLRFAEDR
                     ARASGLPELRLYTHEVMTENVPYYARFGFEETGRRTEDGYSRIFMRKRLPDQW"
     gene            3444..4898
                     /locus_tag="VFU72_07930"
     CDS             3444..4898
                     /locus_tag="VFU72_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5423450.1"
                     /translation="MSPGSTGQGQAASAVGAREIIIFVIVSLALLMSSIDGTIVAVGI
                     PNMMAGLHTNIVWIGWVLTGYSLAQTIVMPMAGKLSDDLGRKRLFLGCVALFTVSSAL
                     CAVAPNVYVLIVCRVFQALGGGAFMPSAAGIVSDVFGEKNRGTAIGLFTSVFPIGGIL
                     GPNIGGWAIDHFGWRSIFAVNLPIGVVLLALGVFLLPSGGRIHQARSIDLVGASAFTA
                     GIVALMYGMTVWGNQIDFSAQIVVWAVVGLAALTFFVWYEGHTREPMIELRLLKEKAF
                     FAANLYNFLYGALVFGFFSFIPLYAIDAYGMSASAAGFILTPRGIAMIVLSTISSFLL
                     VRTGYRAPMIAGLVLISIGLFLVSQGWQNPVIFGYHVGNVALLSALMAITGLGVGVGG
                     PAANNASIELMPEAVALITGLRGMFRTTGGVLGTAAIVLIMAHEPNQAVGLEHIFKGL
                     SILILLAIPPIFLIPNAAGVRPARRGATRMELAE"
     gene            5050..>5260
                     /locus_tag="VFU72_07935"
     CDS             5050..>5260
                     /locus_tag="VFU72_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423451.1"
                     /translation="MRTSLWFVLLLVLLAAGLHYAGNPFTLSVALWFLLAVAIGVILP
                     RWWIVCLTPLPWLLGIGLGLVTGRYL"
BASE COUNT          736 a         1807 c         1801 g          916 t
ORIGIN      
        1 gccggtcgtg gcgctcgata cgccgctcca gctggtcgag ccggcgctcg atgtgccgct
       61 caaggcgctc ctcgtggcgc gtgagtggtc gggatggcgg acccagctcg ccgggcggca
      121 ccggcgggga aacgcggccg ccgaagcgct cggccatctt gcccatgaag gtctgctccc
      181 cgttgagcac ctcgacgttc cccatcagca cgaagccgcg cagccgcacg acgggggcgc
      241 cgggcggcgg tggcggcgac cggcgcttgt cctgctggct ccccatcagc gtgatctctt
      301 cgaaggcgac ctcgacgccc tccggcacca ggatcttcac gtcgcccatg atcacgtaga
      361 cgttgagcac cgaatcgtgg ggaatctccg actggcgcag atcgagccgc aggctggaca
      421 tcagcgagag cgcgttgagc ggcgcgccca ggcgccagcg accctggcgc ttggtatcgc
      481 tcatgatcga gacggtccag tcgggtgccg gctggagcgg cccgcgcggc gtggcgggcg
      541 cggcgggcac ggccgcggcc acggtcgccg gcagatcgcg cgtcagctcg gcgagctggc
      601 cccgggtgcg cgccgccagc gccgcgtcca tgcgctggct gtactcctcc agattgaggc
      661 gcccctcgac gtaggcctca cgcagcccgg acagcgcact ctcgcgctcg gcgtcgctga
      721 ccaggagttc gtcgtcgctc atcgttccca gctcctgtcg cggtatgcgc cgcgctggcg
      781 cggcgtggct ccatcgttag caggatgata ccgaacccgc cgcgccactg ccctcacccg
      841 ctcagccgac ctcggccagc gcccgtcgcg cgatctcgcc ggcctgctcc aacgcgtcgc
      901 tcacctggtt gcgcgcgcgg atcatcgcca cccaccaggt ctggtactcc tccgagtcgc
      961 gggcataggt tgccaacgca ggatagggaa ccagcgccgg gatcatctgc gtctgccagg
     1021 catggccgta gcggtcctgg ccgtagggtc cggcggccgt cgacgcgacc ggcaccagcg
     1081 tgcgcgagag ccagagcttg gcgtggttga gcgcgtccgc cgcctgctgg cccccctcgc
     1141 ccccgtcgcc ggccaggcgc gcctgccagg cgcgcgaggt ctcgtcgagc cgcgcggcca
     1201 ggccgtgaaa ctccttggcg cgctcgaccg cgccggccat gtcgatgccc ggcacgtcga
     1261 gggcggccag gtcgctcagg cgctcgacga agcggcggcc cagcggctcg tactcgtgcg
     1321 gcaggatcgg cgcggtcagg aggccccaga cccagttggc gtagacgcgc aggtgggtgg
     1381 ccagcttgcc cttgtccagc ttgtcgagcg tgttgtggat cgagtggtgc caccagccga
     1441 gcgtggccag cgcggtccgc tggatctcct catcggtgta ggtcatctcg ccggccagcg
     1501 aggccagccc gaccccgaag aaggaggagt cgccgttctt ttgctggcgg cgccagtgga
     1561 ccggcatctc gccgaccatc tccttggccg ccgccaccgc gtagcgctgg atgtcgtcgg
     1621 tcgagtggac gtgccagacc gaggagccgg taatggccgg ctggtcgatc tgcaggtagg
     1681 cgacgcaggc gtggcgcagc cggtcccagt tgacgtcggc gaagcgcgag gaggagatca
     1741 tggtgccggt ctcgtggccc atccagagcc cggccacgat gccgcgccgc aactcgtcct
     1801 gatgctggct gaagacgcgc gccagctcca tgatgcaggc gtcgccggcc gcgttgtcgg
     1861 tcgcctgcgg tccgggccag gagtccatgt gcccgccgag cagcaggaac tggcgcccgc
     1921 gcgccgcacc gaactcggcg ctggtcatcg tcagcggctg ccagccgttg tcggcctggg
     1981 cgttgagcca gacgcgcacc gtcccccgct ggcagagctc cttcagccgc agcccctcgg
     2041 tgcgcgcgat gccgacgcag gggatgtcgg gcatctcctg ctccagggtg gccggcgtcg
     2101 ggttgcccca ggccgacttc atcgagccga aggggaccgc cgggttgtcc ggcgcgcccc
     2161 aattcatcat gatctgggcg ctggagccgc gcttccaggc gatgtaggcc ttctcgtgcc
     2221 gggccggcga ataggagagc tccgagagcg tgatcttgcc ccgcacgtcc ttgccctcgt
     2281 agtccgcctc ggcgcccgag ccgacataga ccagctcgcc ctcgatgccg ggggtcgagg
     2341 ccgagtgccc cagcgtgttg gcctcgatgg tgtacgtctc cggcgccagc acccgtacct
     2401 cggccggctc cgggaaggag accaggccgg ggaagtggtg catggagctg tccagcccgg
     2461 cctcttgaaa gacgtgatag gcgtactcgg ccatgcgccg ggagttctcc gagccggcca
     2521 ggcggctggg gatttggccg gtgatgtgct cgatgtgccg ccaggcgttt tccacggaga
     2581 cctcgcgcaa cacgtcatcc aacgccatcg ccgcctcctc gctcgctcaa atcgctcgac
     2641 atttcgcgct ctgtgcggcg gcaccgtagc agcgccccgc gggcctgtca acgcaggacc
     2701 ggaaggactg gcgtccgcgc tatggctgcc ggagaattgg ccgcaccaca cggcgaggag
     2761 gcggaaggag gcatttcgat gaacgaggat cagacgatcc ggctcgctcg cccggacgaa
     2821 tggggagctg tcctgagctg cgtcgaggac gcctacgcca agtacgtccc gcgcatgggg
     2881 atgaaaccgg cgccgatgct ggccgactac ccggcgctga tcgccaggga cgaggtgtac
     2941 gtcgttcctg ggccggaggg tgtccggggg gtgctggtga tcgagcctcg tgccgaccac
     3001 ctcttcatcg agaatatcgc ggtgcggccc gacgagcagg ggcgcggcta tggctttcag
     3061 ctgctgcgtt tcgccgagga tcgggcccgt gcctcggggc tccctgagct gcgtctctac
     3121 acacacgaag tcatgaccga gaacgtgccc tattacgcgc gcttcggctt tgaggagacc
     3181 ggccgccgca cggaagacgg ctacagccgg atcttcatgc gcaagcggct ccccgaccag
     3241 tggtgaggga attgggggaa ccgcgcccgc gtcctcggtt gacctgacgc ccgccgatat
     3301 ccccccgtcg tccggctcca gctccttgcc cttgggcgta ccattgtaag gctgcgccca
     3361 tcgcatcgga tgccgagcga tgggcgcggc gcgtggttgg tcgatgcaga cggagagtgt
     3421 aagcgggaat gccgcgggtg gggatgagtc ccggttcgac ggggcaggga caagccgcga
     3481 gcgcggtcgg cgcccgggag atcatcatct tcgtgatcgt ctcgctggcg ctcttgatgt
     3541 ccagcatcga cggcaccatc gtggcggtcg gcatccccaa catgatggcc gggctgcaca
     3601 ccaacatcgt ctggatcggc tgggtgctga ccggctacag cctggcgcag acgatcgtga
     3661 tgccgatggc cggcaagctc agcgacgacc tcgggcgcaa gcgcctcttc ctcggctgtg
     3721 tggcgctctt taccgtctcg tcggccctct gcgcggtggc gcccaacgtc tacgtcctca
     3781 tcgtctgccg cgtcttccag gcgctgggcg gcggcgcctt catgccctcg gcggccggca
     3841 tcgtcagcga cgtcttcggc gagaagaacc ggggcacggc cattggcctc tttaccagcg
     3901 tcttcccgat cggcggcatt ctcggcccca acatcggcgg ctgggccatc gaccacttcg
     3961 gctggcgctc gatcttcgcg gtgaatctcc cgatcggcgt ggtgctgctg gccctcggcg
     4021 tcttcttgct cccctccggc gggcgcatcc accaggcgcg cagcatcgac ctggtcggcg
     4081 ccagcgcctt caccgccggc atcgtggccc tgatgtacgg catgacggtc tggggcaacc
     4141 agatcgactt ctcggcgcag atcgtggtct gggcggtcgt ggggctggcg gcgctgacgt
     4201 tcttcgtctg gtacgagggg cacacgcgcg agccgatgat cgagctgcgg ctgctgaagg
     4261 agaaggcctt cttcgccgcc aatctgtaca acttcctcta cggcgcgctg gtcttcggct
     4321 tcttcagctt tatcccgctc tatgccatcg acgcctatgg catgtcggcc tcggcggcgg
     4381 gcttcatcct gaccccgcgc ggcatcgcca tgatcgtgct ctcgaccatc agctcgttcc
     4441 tcttggtgcg cacgggctac cgcgcgccga tgatcgccgg cctggtgctg atcagcatcg
     4501 gcctcttcct ggtgtcgcag ggctggcaga acccggtcat cttcggctac cacgtgggca
     4561 acgtggcgct cctctcggcg ctgatggcga tcaccgggct cggcgtcggc gtcggcggcc
     4621 cggcggccaa caacgcgtca attgagctga tgccggaggc ggtggcgctg atcaccgggc
     4681 tgcgcggcat gttccgcacc accggcggcg tgctgggcac ggcggcgatc gtcctgatca
     4741 tggcgcacga gccgaatcag gcggtgggcc tggagcacat cttcaagggt ctctcgatcc
     4801 tgatcctgct cgccatcccg ccgatcttcc tcatccccaa cgccgccggc gtccgcccgg
     4861 cccggcgcgg cgccacgcgg atggagctgg cggagtagca ggggatccat tcactgaaga
     4921 tccgacgata gcgccacgtc tcattggact acacttgaag taagccctgg cgcgaagggc
     4981 gctacctgtg ttgtccgctc aagtatggcc tccacttgtc acatcaagcg atcgggtaaa
     5041 ggagtctcaa tgagaacttc actgtggttc gtgctactcc tcgttctact ggctgccggc
     5101 ctgcactacg cgggcaatcc gttcacgctc agcgtggcgc tctggttcct actggccgta
     5161 gcgatcggcg tgatactgcc gcgctggtgg atcgtctgtc tgacgccgct gccgtggctc
     5221 ctcggcattg ggctggggtt ggttaccggc cggtatctgt
//
LOCUS       DASUBI010000412         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_385274, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000412 DASUBI010000000
VERSION     DASUBI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385274"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFU72_07940"
     CDS             complement(<1..325)
                     /locus_tag="VFU72_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423452.1"
                     /translation="MRFYLGAHQPHWLEHTARPLFVSEMRLRKRRRLPEARTVWALDS
                     GGFSQLFLHGDWPQGSERVYVEHVRRYVTEIGRLEWAAPQDWMCEPQMIEKTGLSVAE
                     HQRRTV"
     gene            complement(328..1773)
                     /locus_tag="VFU72_07945"
     CDS             complement(328..1773)
                     /locus_tag="VFU72_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5423453.1"
                     /translation="MATTTRRPDYVQAVEKLLPQNMEAEAGVLGSLLIDPSAIMQVAD
                     FLKPADFYSEAHRVIYAAALDLYEAQEPADLLTLCDELSRRGKLEEVGGASFVSSLAN
                     QVPTSRNVKHYAQIVVRCATLRDLIDASGQIAGVAYSEPDAETATEFAVKTVLGIQQR
                     RGVGMASSGRDRSFSEILDELGAETLSRMEADAPTGVLTGLLGLDRLTLGIEPGELVY
                     CAGRPGSGKSALGLGVGVNAARALARSGRPGSVYVVTLEMSARAQARRLVAAAANLNS
                     RYIRAGFRAPGEEPDLEAWTAFDEKREQLKREVGERLWWTEGSVSIAQLRARVMAAVL
                     ERGCRLVIIDQLDLMADSGGQAGEFERITQLSRQLRQIASTYGVAIWCMVQLNRQVEQ
                     RANKRPQLSDLRQSGQLEQDADLVLGIFRPAYYDPERAGKDPRFAQYAKLCVLKARDG
                     ESGVEVHLRFEKEHTRFTDWPYETTPQLSGE"
     gene            complement(1796..2797)
                     /locus_tag="VFU72_07950"
     CDS             complement(1796..2797)
                     /locus_tag="VFU72_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423454.1"
                     /translation="MRQGSPFGIVPTRAVFDERMSPAVLRVLMALATYADEERMCHPA
                     QGTVAKRLHVSRQAVGRCVQQLVALGYIEQVPRYREDGGKMSSAYRIIFDQSPAAATA
                     SRTPMQHDVASPQPEDAPMQHDVASHATSRLHPHATSSVAWGGTREPASSQAGGAVFG
                     GGTDQIEQTIRTEEISVEKEGHSSTISFAADATGADGATPDHPQTAPSSSGLVRRVVR
                     AAGVPLVAERVQRLIEQHPQLREDRLIFEVENAAEWIADPQKNKGGKRKMTVAFLHNW
                     LKRAEQPPPPIALVPAAAASGGHAHGNGNGRYAPPEPPAKLRDAREIKLNALPVALN"
     gene            complement(2873..3334)
                     /locus_tag="VFU72_07955"
     CDS             complement(2873..3334)
                     /locus_tag="VFU72_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423455.1"
                     /translation="MDQTTGPVPGTPPRSARWTQVRAVLLVLLELYARDPLLAGVDRE
                     KLALLAERYAFEPWPGERALPEYVGRRGRLVRWSPDFNEGPQERATRRAMLRRIWGHF
                     VVLSASLATYYDRDEPILTVRFGDGLVTVVYAMDVEWLDDDGQGGDDTPAA"
     gene            complement(3340..3600)
                     /locus_tag="VFU72_07960"
     CDS             complement(3340..3600)
                     /locus_tag="VFU72_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423456.1"
                     /translation="MTRLEFTACRFCGEPMAVQSWAAEGARVCLDCSFRMHLARKRYE
                     SETSEYRRLHADELAEQRRLWRAKRDRRAERNGEDEGEDGKE"
     gene            complement(4011..>4197)
                     /locus_tag="VFU72_07965"
     CDS             complement(4011..>4197)
                     /locus_tag="VFU72_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423457.1"
                     /translation="PITDTEFRRRCRGVGRMDPKQVQELVTAACGEGKAPHRDRCFEA
                     LAAEEQRAAEVAAKVHG"
BASE COUNT          613 a         1646 c         1216 g          722 t
ORIGIN      
        1 gtaccgtgcg gcgctggtgc tcagccacgc tcaatcccgt cttctcgatc atctgcggct
       61 cacacatcca gtcctgcggc gctgcccact ccaggcgccc gatctccgtc acataccgcc
      121 gcacatgctc cacgtagacg cgctctgagc cctgcggcca gtctccgtgc aggaacaact
      181 gcgagaagcc gcccgagtcc agcgcccaga ccgtccgcgc ttccggcagc ctgcgacgtt
      241 tgcgcaggcg catctcggag acgaagagcg gccgcgccgt atgctccagc cagtgcggct
      301 gatgcgcgcc caggtagaag cgcatggtta ctcccccgac agctgcggcg tcgtctcgta
      361 cggccagtcc gtgaagcgcg tgtgctcctt ctcgaaccgc agatgcacct cgacgccgga
      421 ctcgccgtca cgcgccttga gcacgcacag cttggcgtac tgcgcgaagc gggggtcctt
      481 gcccgcgcgc tccgggtcgt agtacgccgg ccggaagatg cccagcacca gatccgcgtc
      541 ctgctccaat tgcccgctct gtcgcagatc gctgagctgc ggacgcttgt tcgcccgctg
      601 ctcaacctgg cggttgagct gcaccatgca ccagatcgcc accccgtagg tcgaggcgat
      661 ctggcgtagc tgccgtgaga gctgagtgat gcgctcgaac tcgccggcct ggccgccgct
      721 gtccgccatc aggtcgagct ggtcgatgat caccagccgg cagccgcgct ccaacaccgc
      781 cgccatcacc cgcgcccgca gctgcgcgat ggagaccgac ccttccgtcc accacagccg
      841 ctcgcccacc tcccgcttca actgctcacg cttctcgtcg aaggccgtcc acgcctccag
      901 gtctggctcc tcgcccggcg cccggaagcc cgcgcgaatg tagcgcgagt tcaggttagc
      961 cgccgccgcc accagccgcc gtgcctgcgc acgcgcgctc atctccagcg tcaccacgta
     1021 cacgctgccc ggccgcccac tgcgcgccag cgcccgcgcc gcgttcaccc ccacgccgag
     1081 ccccagcgcg gatttgccgc tgccaggacg acccgcgcag tagaccagct cccccggctc
     1141 gatgcccagc gtcaagcggt ccagccccag cagccccgtc agcacacccg tcggcgcgtc
     1201 agcctccatc cgcgagagcg tctccgcgcc cagctcgtcg aggatctcgc tgaagctgcg
     1261 gtcacgcccg ctgctcgcca tccccacccc gcgccgctgc tggataccga gcaccgtctt
     1321 gaccgcgaac tcggtcgccg tctccgcgtc cggctcgcta taggccaccc ccgcgatctg
     1381 ccccgacgcg tcaatcaggt cgcggagcgt cgcgcagcgc accacgatct gcgcgtagtg
     1441 cttgacgttg cgcgaggtcg gcacctgatt ggccaggctg ctgacgaacg acgccccgcc
     1501 cacctcttcg agcttgccac gccgtgagag ctcgtcacac agcgtcaaca ggtccgccgg
     1561 ctcctgcgcc tcgtagagat cgagcgccgc ggcgtagatc acgcggtgcg cctcgctgta
     1621 gaagtcggcc ggcttgagga agtcggccac ctgcatgatc gccgacgggt caatcagcag
     1681 gctgcccagc acacccgcct ccgcctccat gttctgcggc agcagcttct cgaccgcctg
     1741 cacgtagtcc ggtcgccgtg tcgttgtcgc cattgctctc tctcctcaag tcagttcaat
     1801 tcagcgccac cggcagtgcg ttcagcttga tctcgcgcgc atcacgcagc ttcgccggcg
     1861 gctccggcgg cgcgtatcgc ccgttgccat tgccatgcgc gtgcccgcca ctggctgccg
     1921 cggctggcac gagggcgatg ggcggtgggg gctgctccgc gcgcttgagc cagttgtgca
     1981 ggaacgccac cgtcatcttg cgcttgccgc ccttgttctt ctgcgggtcg gcgatccact
     2041 ccgcggcgtt ctcgacctcg aagatcagcc ggtcctcccg cagctgcgga tgctgctcaa
     2101 tcagccgctg cactctctcc gccaccagcg gcacccccgc cgcacgcacc acccgccgca
     2161 ccaggccaga gctgcttgga gcagtctggg gatgatcggg agtcgcaccg tcggcgccgg
     2221 tcgcgtcagc ggcaaaagaa atcgtcgaag aatgaccctc tttctcgaca gaaatctcct
     2281 ctgttctaat ggtctgttct atttggtctg ttccgcctcc aaaaacagcc cctccggcct
     2341 gactggaagc gggctcacgc gtaccccccc atgcaacact agatgttgca tggggatgca
     2401 acctggacgt tgcatgggat gcaacgtcat gttgcatggg tgcatcctca ggttgcgggg
     2461 atgcaacgtc atgttgcatg ggggtgcgag aggccgtcgc cgcggccggc gactggtcga
     2521 agatgatgcg atacgcgctg ctcatcttgc ccccgtcctc gcggtagcgt ggcacctgct
     2581 cgatgtagcc cagcgccacc agctgctgca cacagcgccc caccgcctgc cgcgagacgt
     2641 gcagccgctt ggccaccgtc ccctgcgccg gatgacacat ccgctcctca tcggcgtacg
     2701 tcgccagcgc catcagcacc cgcagcaccg ccggactcat ccgctcgtcg aagacggcgc
     2761 gggtcggcac aatgccaaac ggtgatccct gcctcatgcc tgcccgcctt tgtctctgcc
     2821 gctactgctc atcgtctcgc cccccaccca ctccatcccc accacacgcc gtttatgccg
     2881 ccggcgtatc gtctccgccc tggccgtcat cgtccagcca ctcgacgtcc atcgcgtaga
     2941 ccactgtcac caggccgtcg ccgaagcgca ccgtcaggat cggctcgtcg cggtcgtagt
     3001 acgtcgccag gctcgccgag agcaccacga agtgccccca gatgcgccgc agcatcgccc
     3061 gccgcgtcgc gcgttcctgc ggcccctcgt tgaagtcggg cgaccagcgc accagccgcc
     3121 cccgccggcc aacatactcc ggcagcgccc gctcccccgg ccacggctcg aacgcatagc
     3181 gctccgccag cagcgccagc ttctcccggt ccacgcccgc cagcagcgga tcccgcgcat
     3241 agagctcgag cagcaccaac agcaccgccc gcacctgcgt ccatcgcgcc gaacgcgggg
     3301 gtgtccccgg caccgggcct gtcgtctggt ccatcgcgcc tactccttcc cgtcctcgcc
     3361 ctcgtcctcg ccgttgcgct cagcacgccg atcgcgcttc gcccgccaca gacgccgctg
     3421 ctccgcgagc tcgtctgcgt gcaggcggcg gtactccgac gtctccgact cgtagcgctt
     3481 ccgcgccagg tgcatccgga acgagcagtc cagacacacc cgcgccccct ccgctgccca
     3541 actctgcacc gccatcggct ctccgcagaa gcggcacgcc gtgaactcca gtcgcgtcat
     3601 ccccgtcgcc tccgtcctcc gcccctgcgg acaaatctcc ctttgcgggc tagtctcgta
     3661 tctccgtggg atggcagtag cacccacact gccaggcacc ggcgcgacag tcccgatggt
     3721 ggccgctctg gcagcttgcg aggatgggcg catgctgatg tgcatggcgg tggcacgcct
     3781 cgacagctcg ccagcgggat gcgtaggttg tgaccccctg ccgggtcgtg tcaatcggca
     3841 tgccgacgct aaacccggtg ccgtcgcgtt tgaggccgta atagtaaccg gcggcgttct
     3901 ttgcgatctt gtagcctcgt ggcggctgct gcgtgtggtt gcccaaatgc accttgcgat
     3961 gtggcatctt ctgtctctcg cctccaaact gctggtactt tgtccgcggc ctacccgtgc
     4021 accttggccg cgacctccgc tgcccgctgc tcctccgccg ccagcgcctc gaagcaccga
     4081 tcgcggtgcg gcgccttgcc ctcgccacag gccgccgtca ccagctcctg cacctgtttg
     4141 gggtccatac gccccacgcc tcggcagcgt cgccggaact cggtgtcggt gatcggg
//
LOCUS       DASUBI010000413         5701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_41097, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000413 DASUBI010000000
VERSION     DASUBI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41097"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFU72_07970"
     CDS             <1..1666
                     /locus_tag="VFU72_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5423458.1"
                     /translation="FSSKRGTPMATLPPLTVTASPTAVSVGTPAATPAIPPAGTPSVI
                     RAATPTAQPQPRGAQTLTLTGSVEDPTTLDPALVREASAGFIDQQIFRGLVHLNSDLT
                     VSPDLAQTIDVSSDHQIFTFHLYPDLTFQSGRPLHAADVQYSLERATDPALAKQSGQP
                     LPAAIYLSDIRGASERLQGKTTPLSGVEVLDPLTVRITLTHPTADFLQKLTGLPAAVV
                     SRANVEQGGEWWRQPDGSGQFKLTSWKSGAQMVLAGVSSYRPAPPTLKTVTILLGASA
                     QQPVALYERDQIDVAPVSDDILPRVLAPGTPLHDQLQTEPTLGGEYVFINPNVPPFSD
                     PKLRQALIQGFERSKIANVTFEGHVSVLDGIVPVTGDSHTWAAKLPPYDLGSAKQLYQ
                     QVSDQARAQPLTLYTAGSWTPAAMKQVYQRDLGINAEVVDLDWSDYIQRLAKQNMGMF
                     SLNWIADYPDPGSVLWSLFASTSPDNPIGYHNPEVDRLLARAEAEPDPQRRIDLYRQA
                     QQLIVDDNVVIPIYRDTDYELVKPRVHGLQFSPLGMLGLETVWIAG"
     gene            1689..2594
                     /locus_tag="VFU72_07975"
     CDS             1689..2594
                     /locus_tag="VFU72_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423459.1"
                     /translation="MVDGEVHGGEHEPRGRGARIEPLTQSTLTLLRLGWSSRFDLAEL
                     SANLRQHPGLSLWIPETGEYLIGGSWRHRGEVAGMLELSARANAIPVLDEFAEIARDE
                     GKQLAIASEHHESRRRAFYESAGFELLEDIVIYELARGLPPSPGGALQLQSVDVNDEL
                     MLAELTALDHAAFPWLWWNSREEFENYGGSPGVELYLFRDNSGAAVAYIGITHFRDWG
                     HLDRIAVAPGLQGRGYGREALDWAVHFLSRAGARRIGLSTQARNTRSRQLYERYGFRR
                     APVQDYALYGRWLVTPPEEFRAGRG"
     gene            2604..3671
                     /gene="aroC"
                     /locus_tag="VFU72_07980"
     CDS             2604..3671
                     /gene="aroC"
                     /locus_tag="VFU72_07980"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5423460.1"
                     /translation="MGSTFGKVFRITTWGESHGPALGVVVDGCPAGLALSEELIQHDL
                     DRRRVGQSKVTSPRNERDKVTILSGVFEGVTTGAPISLITYNADADSSKYDQIRDLFR
                     PGHGDYTYWTKYGVRDHRGGGRSSARETWGRVAAGAIARELLRVTAGIDIYGFVRQLG
                     PIVMETFERGEIDQNLVRCPDPQAAAQMVALVMDAKEDHDSVGGIIEIRAENVPAGLG
                     EPTMDKLDGLLGHAMLSIPAVKGVEIGLGFDAVQLRGHEHNDGFYIENGRVRTLSNNA
                     GGTLGGISSGEPVVCRIVVKPTSSVAQEQRTVDIYGQERTVLVEGRHDPSICPRAVPV
                     AEAMMALVLADLYLQNRAARV"
     gene            complement(3878..5182)
                     /gene="aroA"
                     /locus_tag="VFU72_07985"
     CDS             complement(3878..5182)
                     /gene="aroA"
                     /locus_tag="VFU72_07985"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5423461.1"
                     /translation="MAQYPDALLIAPVRGPVDAAVELPGSKSYTNRALLIAALAEGDS
                     AIRAALFSDDTYYMAESLTRLGIAVAADERSATFHVVGQGGRIPAERADLFAGNSGTT
                     ARFLTAALALGHGAYRLDGVPRMRQRPIEPLLTALRQLGVDAASEMGDGCPPLLVRGT
                     GLRGGKAALPGQTSSQYFSALLMVGPLTRDGLTLEVEGDLVSQPYIDLTAGTMRAFGA
                     EMRNEDYRRFVVAGGQRYVGRDYAVEPDASAASYFFALAAATGGRVRVANLGAGSAQG
                     DVHFVDVLEQMGCRVERAADFIEVAGPAQLRGADIDMNGISDTVQTLAAIAPLAEGAV
                     TIRNVGHIRHKETDRLAALVAELRGLGVMVEEHADGLTVLPGFVRPGRVRTYDDHRMA
                     MSFAILGCIVPGITIEQPACVAKTFPDFFARLAAATGQDAPF"
     gene            complement(5198..>5701)
                     /locus_tag="VFU72_07990"
     CDS             complement(5198..>5701)
                     /locus_tag="VFU72_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU5423462.1"
                     /translation="PNCGSGRPRESEEGFGVIDGVEIKRLTTHADERGALTELIRRDD
                     PFFEQFGQCYVSVSYPGVIRGWHYHKRQTDYFTCVRGMIKVPLFDRREDSPTFGELNE
                     FFIGDDNRIVVKIPIGVLHGFKNIGAEPCYLINFPTQPYDPKDPDEYRIPFDSPDIPY
                     SWDISFH"
BASE COUNT          974 a         1883 c         1948 g          896 t
ORIGIN      
        1 gttcagcagc aagcggggca caccgatggc caccttgccg ccattgacag taacggcatc
       61 gcctaccgcg gtctcggtcg gcactccggc ggccacgccc gccattccgc ccgccggcac
      121 gccgagcgtg atccgcgcgg ccacgccgac cgcgcagcca caaccgcgcg gcgcgcagac
      181 gctgaccttg accggcagcg tcgaggatcc caccacgctc gatccggcgc tggtgcgcga
      241 agccagcgcg ggcttcatcg accagcagat cttccgcggt ctggttcatc tcaacagcga
      301 tctcaccgtc tcgccggacc tggcacagac gatcgacgtc tcctccgatc accagatctt
      361 cacgttccac ctctaccccg atctgacatt tcagagcggt cgcccgttgc acgccgccga
      421 cgtccagtac tccctggagc gggccactga tccggcgctg gcgaagcaga gcggccagcc
      481 gctgccggcc gcgatttacc tgtcggacat tcgcggcgcc agtgagcggc tgcaagggaa
      541 aaccacaccg ctcagcggcg tcgaggtgct cgatccgctg accgtgcgca tcacgctgac
      601 gcaccccacc gccgacttcc tgcagaagct gaccgggctg ccggcggcgg tcgtcagccg
      661 ggccaacgtc gagcaggggg gcgagtggtg gcgccaaccg gacgggagcg gacaattcaa
      721 gctgacgagt tggaagtccg gtgcgcagat ggtcctcgcg ggcgtatcga gctaccggcc
      781 cgcgccgccg acgctgaaga ccgtgacgat actgctgggc gcgtcagcgc agcagccggt
      841 ggcgctgtat gagcgtgatc agatcgacgt ggcgccggtg tcggacgata tcctgccacg
      901 agtgttggcg ccgggcacgc cgctgcacga ccagctacag accgaaccaa ccctgggcgg
      961 cgagtacgtc ttcatcaatc ccaacgtgcc gccattctcc gacccgaagc tgcgccaggc
     1021 cttgatccag ggcttcgagc gcagcaagat cgccaatgtg accttcgaag gccacgtgag
     1081 cgtgctcgat gggatcgtac cggtcaccgg cgatagccac acctgggccg cgaaactgcc
     1141 gccctatgat ctgggcagcg ccaagcagct ctaccaacag gtcagcgatc aggcgcgcgc
     1201 gcagccgctg acgctgtaca ctgcggggag ttggactccg gcggccatga agcaggtcta
     1261 ccagcgggat ctggggatca atgccgaggt ggttgatctc gactggagcg actatattca
     1321 acgactggcg aagcagaaca tggggatgtt ctcgctcaat tggatcgccg actatcccga
     1381 tcccggcagt gttctctgga gtttgttcgc ctcgacaagt ccggacaacc cgatcggtta
     1441 tcacaacccg gaggtcgatc gtctattggc gcgcgccgag gcagagccgg atccacagcg
     1501 acggatcgat ctctatcgcc aggcgcaaca gctcatcgtc gacgacaacg tggtcatccc
     1561 gatctaccgg gataccgact acgagctggt caagccgcgg gtccacggtc tgcagttctc
     1621 acccctgggg atgctcgggc tggagacggt gtggatcgcg ggctaggctg gcccagggga
     1681 gtacggtcgt ggtggatgga gaagtgcacg gcggcgagca cgaaccgcgc gggcggggag
     1741 cgcgcatcga gccacttacc cagtccacgc tgacactgct gcgcctcggt tggtccagcc
     1801 gcttcgacct ggcagagctg agcgccaacc tgcgccagca tccggggctc tcgctctgga
     1861 ttcctgagac cggcgaatac ctgatcggcg gctcctggcg ccaccgggga gaggtggccg
     1921 gcatgctgga gctgagcgcg cgtgccaacg ccatccccgt gctcgacgaa ttcgccgaga
     1981 tcgcccgcga cgagggcaaa caactcgcca tcgccagcga gcaccacgag tcgcggcggc
     2041 gcgccttcta cgagtcagcc ggcttcgagc tgctggagga catcgtcatc tacgagctgg
     2101 cacgcgggct accgccgtcg ccgggcggcg cgctccagct gcagtcggtc gacgtcaacg
     2161 acgaattgat gctggccgag ctgaccgcgc tcgaccatgc cgccttcccc tggctctggt
     2221 ggaacagccg cgaggagttc gagaattacg ggggcagccc gggggtggaa ctctatctgt
     2281 tccgcgacaa ctcgggcgcg gccgtagcct acatcggtat tacgcacttt cgcgattggg
     2341 gtcatctcga ccgcatcgcg gtcgcgccgg ggctgcaagg acgcggctac ggtcgggagg
     2401 cgctcgactg ggccgtgcac ttcctctcgc gggccggtgc gcggcggatc ggcctgagca
     2461 cgcaagcccg caatacgcgc agccggcaac tctacgaacg ctatggtttc cgccgcgcgc
     2521 cggtgcaaga ctacgcgctg tatggccgct ggctggtgac gccgcccgag gagttccggg
     2581 cgggaagagg atgagcaggg gagatgggca gcacgttcgg taaggtcttt cgtatcacta
     2641 cctggggcga gtcgcacgga ccggccctcg gggtcgtcgt cgacggctgt cccgcgggac
     2701 tagcgctctc ggaggagctg atccagcacg atctcgatcg acgccgggtg gggcagagca
     2761 aggtcacctc accgcgcaac gagcgggaca aggtgacgat cctctccggt gtcttcgagg
     2821 gtgttacgac cggtgcgccg atctcgctga tcacctacaa cgccgacgcc gactccagca
     2881 agtacgacca gattcgcgac ctcttccggc ccggccacgg cgactacacc tactggacca
     2941 agtacggcgt gcgcgaccac cgcggcggcg gccgctccag cgcgcgcgag acctgggggc
     3001 gcgtggcggc cggcgccatt gcccgtgagc tgctgcgggt gacggccggc atcgacatct
     3061 acggcttcgt gcgccagctc ggcccgatcg tgatggagac gttcgagcgc ggcgagatcg
     3121 accagaacct ggtgcgctgc cccgatccac aggcggcggc gcagatggtc gcgctggtga
     3181 tggatgccaa ggaggaccac gacagcgtcg gcggcatcat cgagatccgc gccgagaacg
     3241 tgccggcggg gctgggcgag ccgacgatgg acaagctcga tggcctgctt gggcatgcca
     3301 tgctgagtat cccggcggtc aagggagttg agatcgggct gggcttcgac gcggtccaac
     3361 tgcgcggaca cgaacacaac gacggcttct acatcgagaa cggtcgcgtg cgcacgctct
     3421 cgaacaacgc cggtggcacg ctgggcggca tctcatccgg cgagccggtc gtctgccgca
     3481 tcgtggtcaa gccgacgtct tcggtggcgc aggagcagcg cacggtcgac atctacggcc
     3541 aggagcgcac ggtactggtc gaggggcgac acgacccgtc gatctgcccg cgcgcggtgc
     3601 cggtggccga ggcgatgatg gcgctggtgc tcgctgattt gtacctgcag aaccgggcag
     3661 cgcgggtcta ggtttgggtg cctgacgacc gcgttgggtg ccgagcgagg cacggatcct
     3721 tcgcctgcgc tcagagcctg acttgagcct gtcgaaggga tgacaagggg aggggatgac
     3781 ctaccggacg acacgagccg tcctaatccg cctcgtacta cccctgccat cccgaccgaa
     3841 gtggagggat ccgtcatgcc cggcgacgca gcgtcgctta gaacggggcg tcttgccctg
     3901 tggcggcggc taggcgcgca aagaagtcgg ggaaggtctt ggcgacgcag gcgggctgct
     3961 caatggtgat ccccggcacg atgcagccga ggatggcgaa gctcatcgcc atgcggtggt
     4021 cgtcgtaggt gcgcacgcgc ccggggcgca cgaagccggg gaggacggtc agcccgtcgg
     4081 catgctcctc gaccatcacg ccgaggccgc gcagttcggc caccagcgcc gccagccggt
     4141 ccgtctcctt gtgccggatg tggccgacgt tgcgaatggt caccgcgcct tcagccagcg
     4201 gcgcgatcgc ggccagcgtc tgcaccgtat ccgagatgcc gttcatgtcg atgtcggccc
     4261 cccgtagctg ggccggacct gccacctcga tgaagtcggc agcacgctcc acccggcagc
     4321 ccatttgctc cagcacgtcg acgaaatgca catccccctg cgccgagccg gcgccgaggt
     4381 tggccacgcg aacgcgcccg ccggtcgcgg cggccagggc aaagaaatag gaggcggctg
     4441 aggcgtccgg ctcgacggcg tagtcgcgcc cgacgtagcg ctggccgccg gccacgacga
     4501 agcgccggta gtcctcattg cgcatctcgg cgccgaaggc gcgcatcgtg ccggccgtca
     4561 ggtcgatgta cggctgagag accaggtcgc cctcgacctc cagcgtcagt ccatcgcgcg
     4621 tcagcggccc gaccatgagg agcgcgctaa agtactggct gctggtctgt cctggaagag
     4681 ccgccttgcc gcctctcagc cccgtgccgc gcacgagcag cggcgggcag ccgtcgccca
     4741 tctcggaggc ggcgtcgaca ccgagctggc gcagcgcggt gagaaggggt tcgatcggac
     4801 gctgacgcat gcgcggcacg ccgtcgagcc ggtaggcccc gtggccgagc gccagcgctg
     4861 ccgtcaggaa acgagccgtg gtgccggagt tgccggcgaa caggtcggcg cgctcggcag
     4921 ggatgcgccc accctgaccg acaacgtgga atgtggccga tctctcgtcg gccgcgaccg
     4981 cgatcccgag ccgggtcagg ctctcggcca tgtagtaggt atcgtcgcta aagagagccg
     5041 cgcggatcgc cgagtcgccc tccgccagcg ccgcgatcaa cagcgcgcgg ttggtgtagc
     5101 tcttggagcc gggcaactcg actgccgcgt cgaccgggcc gcgcaccggc gcgattagga
     5161 gggcgtctgg gtactgcgcc atgcggccgt cctcgcgcta gtggaagctg atgtcccaag
     5221 agtaggggat gtcgggcgag tcgaagggga tgcggtactc gtcaggatct ttgggatcgt
     5281 agggctgggt cggaaagttg atcaggtagc agggctcggc gccgatgttc ttgaaaccat
     5341 ggagcacgcc gatcgggatc ttgaccacga tgcggttgtc gtcgccgatg aagaactcgt
     5401 tcagctcgcc gaacgtgggc gagtcttcgc ggcggtcgaa gagcggcacc ttgatcatgc
     5461 cgcgcacgca ggtgaaatag tcggtctgcc tcttgtgata gtgccagccg cggattacgc
     5521 ccgggtagct gaccgagacg tagcactggc cgaactgctc gaagaagggg tcgtcgcgtc
     5581 ggatcagctc ggtcagcgca ccacgctcgt cggcgtgcgt cgtcagccgt ttgatctcca
     5641 cgccgtcaat cacgccgaac ccttcctcac tctcacgcgg acgacccgaa ccgcaattcg
     5701 g
//
LOCUS       DASUBI010000414         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1081537, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000414 DASUBI010000000
VERSION     DASUBI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081537"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..538)
                     /locus_tag="VFU72_07995"
     CDS             complement(224..538)
                     /locus_tag="VFU72_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423463.1"
                     /translation="MHGECGRQCFRRSRRKIGRRGRYYRPTITQNAGQGLHERLVALL
                     GAGRISCPPPGKPTGIAQFHLEAQRRIFAFCRGVRPFVIVKRARMLEVLAACQEKYGW
                     HV"
     gene            1039..2067
                     /locus_tag="VFU72_08000"
     CDS             1039..2067
                     /locus_tag="VFU72_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU5423464.1"
                     /translation="MLGHLRRQPNGMTSGELAEVLGVDASTIRRDLARLRVTDAGLRR
                     RGRRYVVDFQRAQRPLRLSADEVMALYLACRLLTRQQGDRNPHAEAAMRKLADTVRDD
                     APRLARYLDDAAVIAHTLPLRAGFQSVLEVLTHAISDGHIVTLRYHDRHGVVSERRFH
                     PYAVEPFGETHSCYAIGFDETRGAIRTFRLDRIENATLTADPFELPPSFDPSTLFAEA
                     WGVVWSEAETQLVTLRFVGEGAVEVQERSWHPSQRIVPHADGSCVVSFRVSAPLELRR
                     WILQWGVDVEVVAPPELRAAIAGEATRIARRAAEAAEATDEDVEHRAGDPRVAPSNRP
                     LDAPASSP"
     gene            2339..>4819
                     /locus_tag="VFU72_08005"
     CDS             2339..>4819
                     /locus_tag="VFU72_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423465.1"
                     /translation="MATYGDYYQRAIDTGAPGVKLVLGPTGLGKTSGITPTVDANPDR
                     KFLYLANRKQLLDQLAQSFAPAACRVLRRDLETVQHVLSTQRPAYDELLAARQFREGL
                     RAAQQHIRLKLLDPVAIRRASDELAELDLTDRHLPPFLSERAADQAHQLLTAFRLVLT
                     VARDRAEQNERYRWLAGHAVMESLFPAIPFRRRPEVRILLMTLQKAYYGFYDGVATRN
                     LSNLVDDERTVIFLDEFDFLEHDLVNMICRAPQVQDPFDFTAHFYRAMARHKLPHPDF
                     PQHNDQNIRHRIGAIVKLVDGVQARGLDFPEINQFTLARPDADAVNAKPNRRSRAPAI
                     FRTRHTVSTNPLYLKPTERSFQLEFESRDPSWPHASWLFNAVGTATTRILALFKELQR
                     ENERWYLEMMRHCFRNSVFYDQVSAVAQFPRRAGQVSGPRGELLDSGYSLFDIDQLDQ
                     VTDSEEVGVVFYQMLQTPENLLRALAQRHLVFGLSATADLPRCVHHFDLGWLEEQGLL
                     LPTTDADRADIERMIAEKAALRGGGMALAVIDGLQAADAEQAAMQRFLDAVAGDDEFG
                     DDTAGGHRSQRMHRFFAAVRWVLTRTGQGQGQAWPPRLLLFLNSFRQVRLLLTTFAEH
                     AAAAGVFSVEPLAVGESSTGSSERSSAGNGKNREFQAFRLTLWGRRLIVILFDAAFAA
                     EVRQDVEASAAFKALFHTAEPIIVATQYLSAGNGVNLTYTDEPGSPERDFTHIALLEA
                     PYFFFAKPDEDMTPAEVFASRKANIWYQAKLFYARQISEPRFKQVLATLARPGEWNEH
                     YRSGSTATDCLLSQLAIFIQALGRVERGR"
BASE COUNT          782 a         1680 c         1555 g          802 t
ORIGIN      
        1 gagtcgcggc tagcaggggc ggagagagag ccactcatcc cgtgctcacc cacagacgcg
       61 ggagcaagag gcagcggagt agggggccta gacgacaggg acctcctgcc cggccaaaat
      121 ctctcgagcg cgagggctgg ctgatcgtct cggcccctcc cactacgccc tcaatagccg
      181 cgccgcaagc gcaccgaaca ccgacccgcg agcgcctgac ttatcagaca tgccacccat
      241 acttctcctg acaggcggcg agcacctcga gcatccgcgc ccgcttgacg atgacaaagg
      301 gccgcacccc gcgacagaac gcgaagatcc gccgctgcgc ctccaggtgg aactgggcga
      361 tgccagtggg cttgccaggt ggtgggcatg agatccgccc ggctccgagc agcgccacca
      421 gccgctcatg cagcccctgc cccgcgttct gcgtgatcgt cggccggtag tagcggccac
      481 gccggccgat tttgcggcgg ctgcgccgga agcactggcg cccacattcc ccgtgcattc
      541 cccgtgcgcg cgggacgctt ggtgttcaga tcgcagcttg ctcgccgcgc gtcgatgtgg
      601 acgggcgggc ccctggggcc gcacggtcat ccgtcaggtg tggggcggtg acgcaggatg
      661 tctcaccagg cgggccgttt gtcctggccc atgtgctctg gccgtggcta gggtgaccca
      721 cggtgaccaa tgcggtggcg cgcggagctg acgatgaccc tgagagtgag ccgtcgtcct
      781 ccccgcggtc gacgtggccc cggcaccaca ccacgtatgc cgcatggcgc tgagcgagtg
      841 gacaccgtca ggagctcgcc gcaccagacg ggccgtccgc tcgcccataa atcgtgcaaa
      901 tgttgcgcga tttgcgcgag tatggtagac tcctgatgcg agcgctcgac gctgcgggag
      961 cgcaggggtc tcttgggggg ccgtcctcaa gggaggaggg ttgcggtgga cagcacgaca
     1021 cagcggcgcc acgaggagtt gctcggccac ctgcgccggc agccgaacgg gatgacctcg
     1081 ggtgaactgg ccgaggtgct cggcgtggac gccagcacca tccggcggga tctggcgcgc
     1141 ctgagggtca ctgatgcggg gttgcgccgc cgcggccgcc gctacgtcgt ggacttccag
     1201 cgtgcacagc gccccttgcg cctgtcggcg gatgaggtga tggcgctcta tctcgcctgc
     1261 cgtcttctga cgcggcagca gggcgaccgc aatccgcacg ctgaggcggc gatgcgcaag
     1321 ctcgccgata ccgtgcgtga cgatgctcca cgcctcgcac gctacctcga tgatgcggcg
     1381 gtcatcgcgc ataccctgcc gctgcgcgcg ggattccaat ccgtcctgga ggtgctaacc
     1441 cacgccatct ctgacgggca catcgtcacc ctgcgctacc acgatcgcca cggcgtggtc
     1501 agcgagcggc gcttccatcc gtacgcggtg gagcccttcg gcgagaccca tagctgctat
     1561 gccatcggct tcgatgagac gcgtggtgct atccgcacct tccgcctgga tcgcatcgag
     1621 aacgcgacgc tgaccgccga cccgttcgaa ctgccgccgt ctttcgaccc cagtacgctc
     1681 tttgccgagg cgtggggagt cgtgtggagc gaggccgaga cgcaactcgt cacgttgcgc
     1741 ttcgtcgggg aaggggctgt ggaggtccag gaacgctcgt ggcaccccag ccagcggatc
     1801 gtcccacacg ccgacggatc gtgcgtcgtg tccttccgcg tgtcggcgcc cctcgagttg
     1861 cgtcgctgga tcctccagtg gggcgtggat gtcgaggtcg tcgcacctcc cgagttgcgc
     1921 gcggccatcg cgggcgaggc cacacgcatc gctcggcgcg ctgctgaggc ggcggaggcg
     1981 acggatgagg acgtggagca ccgtgcgggt gatccccgtg tggccccatc taaccgaccg
     2041 ctcgatgctc cggcctcctc accgtaggcc catcccgctc ccgctccagt ccctggaaac
     2101 atcggtcggt agccgctgct gccacggctg ccactgcggg gcctacagcc gccgctggcc
     2161 caaaaccctc gtctgtcccc cctgaagatc gtcccgtcgc cggactagta gcggcaccgt
     2221 cggcgcggcg cctctcgcac cgtttacgtc tctccttggc ccaggacccc gctcacgcgg
     2281 gagagcccgc cgggggccgt tcgcgtatgg ctcgtccgta caccgggagg ggtgtcacat
     2341 ggccacctat ggcgactatt atcagcgggc aattgacacg ggggcacccg gcgtcaagct
     2401 ggtgctcgga cccactgggc tgggcaagac gagcgggatc acgccgaccg tcgacgccaa
     2461 tcccgaccgc aagttcctct acctcgccaa ccgcaagcag ctactcgacc aattggccca
     2521 gagcttcgcg cctgctgcct gccgcgtcct gcggcgcgac ttggagaccg tgcagcacgt
     2581 gctctcgacg cagcgcccgg cctacgatga gttgctcgcc gcccggcagt ttcgtgaggg
     2641 cctgcgggca gcacagcagc acattcgctt aaaactgctc gacccggtgg ccatccggcg
     2701 cgccagcgac gagttggccg agctcgatct caccgatcgg cacctgccac ccttcctttc
     2761 agagcgtgcc gccgaccagg cacaccagtt gttgaccgcg ttccgtctcg tgctgacggt
     2821 agccagggat cgcgcggaac agaacgagcg ctatcgttgg ctggcagggc atgcggtgat
     2881 ggagtcgctc ttcccggcca tccctttccg ccggcggccc gaggtgcgca tcctgctcat
     2941 gacgctgcaa aaggcctatt acggcttcta cgacggcgtc gcgacgcgca acctgagcaa
     3001 cctcgtcgac gacgagcgca ctgtcatctt tctcgacgag ttcgacttcc tcgagcacga
     3061 cctggtgaac atgatctgtc gcgcgccgca ggtccaggat cccttcgatt tcacggcgca
     3121 cttctaccgg gcgatggccc ggcacaagct gccccacccc gacttccccc agcacaacga
     3181 ccaaaacatc cgccaccgca tcggggccat cgtcaagctc gtagacggtg tccaggcgcg
     3241 aggattggac ttccccgaaa tcaaccagtt caccctggcg cgccctgacg ccgacgctgt
     3301 caatgccaag cccaacaggc gctcccgcgc gccggccatt ttccgcaccc gccacaccgt
     3361 cagcaccaac ccgctctatc tcaagccgac cgagcgctct ttccagctgg agttcgagtc
     3421 acgtgatccg agttggccac acgcctcgtg gctcttcaac gcggtcggca cggccaccac
     3481 gcgcattctc gcgctcttca aagaactgca gcgtgagaac gagaggtggt acttggagat
     3541 gatgcgccac tgcttccgca acagcgtctt ctatgaccag gtgagtgcag tggcccagtt
     3601 cccccggcgc gcggggcagg tgagcgggcc gcggggcgag ttgctcgaca gtgggtacag
     3661 cctcttcgac atcgatcagc tggatcaggt cacggacagt gaagaggtgg gggtcgtctt
     3721 ctatcagatg ctgcagacgc cggagaatct gctgcgtgcg ctggcccaac gccatctcgt
     3781 cttcggtctc tcggccaccg ccgacctgcc gcgctgcgtc caccacttcg atctgggctg
     3841 gctcgaagag cagggcctgc tgctgccgac gacggacgcc gatcgcgccg acatcgagcg
     3901 catgatcgcg gagaaggcgg cgctgcgcgg tggcggcatg gcgctcgccg tcatcgatgg
     3961 cctccaagcc gccgatgctg aacaggcggc gatgcaacgc ttcctcgatg cggtcgccgg
     4021 cgatgacgag ttcggcgacg atactgcggg cgggcaccgc agccagcgga tgcatcgctt
     4081 cttcgccgcg gtgcgctggg tgctgacgcg gacggggcag gggcaggggc aggcctggcc
     4141 gccacgcctg ctcctcttcc tcaacagctt ccgtcaggtg cgcctgctcc tgacgacttt
     4201 cgcggagcac gccgccgcgg ccggcgtgtt cagcgtcgag ccgctcgccg taggggagag
     4261 cagcactggc agcagcgagc ggagcagcgc agggaacggg aagaaccggg agttccaagc
     4321 cttccgcctc acactgtggg gccgtcgcct gattgtcatc ctcttcgatg ccgccttcgc
     4381 ggccgaggtg cggcaggatg tggaagcgag cgccgcgttc aaggcgctct ttcataccgc
     4441 cgagcccatc attgtcgcga cgcagtacct ctcggctggc aacggcgtca acttgaccta
     4501 caccgacgag cccgggagcc ccgagcgcga cttcacgcat atcgcgctgc tcgaggcgcc
     4561 ctacttcttt ttcgcgaaac ccgacgagga catgacgccg gcggaggtct tcgccagtcg
     4621 gaaggccaat atctggtacc aggccaagct cttttacgcg cggcagatct cagagccgcg
     4681 cttcaaacag gtcctggcga ccctcgcacg gccgggtgag tggaacgaac actaccgctc
     4741 cgggtccacc gcgaccgatt gcctgttaag ccagctggcg atctttatcc aggcgctcgg
     4801 ccgcgtcgag cgcggccga
//
LOCUS       DASUBI010000415         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_586585, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000415 DASUBI010000000
VERSION     DASUBI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586585"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFU72_08010"
     CDS             complement(<1..639)
                     /locus_tag="VFU72_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5423466.1"
                     /translation="MQERFDVVIVGAGPAGCVLAARLSEDPGRRVLLLDAGPDYGPDL
                     AAWPAELLEPYHSEVESHSWGYTNRADGAGRKLALPRARVVGGCSAINACLWYRGSRV
                     DYDGWAAAGNPGWGYDDLEPYFRRAESDPDGRPGYHGLTGPVRISRAPLLSPLDEAML
                     ETAEELGFPLLEDLNGSPEQIPCAGRTPKNIVEGVRWSAALSYLALARPRPNF"
     gene            complement(733..1764)
                     /locus_tag="VFU72_08015"
     CDS             complement(733..1764)
                     /locus_tag="VFU72_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5423467.1"
                     /translation="MADAVRVEGEAAPWSAPQAERTDVTAWLFREQQEARQVAVADLP
                     DLVTQDPNFVWIDLSEYSQSELRHLAELLGLPTLAVHTALSVWQRPRLDVFDHQFFVA
                     VTVPSRDSTAYRVEASELDLFIGHNFLVSAHKRPLRFMARVLARARQRPELASLDAAF
                     MLYLVLDELLAYGEGLSEGLENEIEQMEERALKDTSDTFLDDLLRLKRYSFAVSRLLD
                     HHEELFAAFLRPDFSFVSGGEVEPYFRDLDARLARLLDKIRATREAVNGAFDIYVSQM
                     SHRTNQVMKILTLVSTVLLPATVILGVFGTNFQGVPLYTSTAFLVMLALIVLISGVIL
                     LAFRQRGWF"
     gene            complement(2054..>2764)
                     /locus_tag="VFU72_08020"
     CDS             complement(2054..>2764)
                     /locus_tag="VFU72_08020"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEU5423468.1"
                     /translation="RHLQFSQRAADAAESALPEALAVLSPGKSVVTIDVATGQRTIAR
                     RLVSQGADYVVTLNGNAERLTSGIAAVFSQTETGGFASVAHDYDETEPSGHDGGERRR
                     AWLITDPTYLAPLNERAHWPRLNSIGLVEREIDSGGVVNREQRYYLSSLEGNARRLAD
                     AVPPPRQEPRGPSWRLDIDFGAETNLPFGHAGRNLAQLRRIALRLLREERTGRGGVTA
                     RRLTARRDERYLLAVLAG"
BASE COUNT          419 a          958 c          986 g          401 t
ORIGIN      
        1 gaagttgggg cgcgggcggg ccagggccag atagctcagc gcggcactcc agcgcacccc
       61 ctcgacgatg ttcttcggcg tgcgcccggc gcaggggatc tgctccggcg agccgttcag
      121 gtcctccagc agcgggaagc ccagctcctc ggccgtctcc agcatggcct cgtcgagcgg
      181 cgagagcaac ggggcgcgcg agatgcgcac cggcccggtc agaccgtggt agccgggccg
      241 cccatcggga tcggactcgg cgcggcgaaa gtacggctcc aggtcgtcgt agccccagcc
      301 ggggttgccg gccgccgccc agccgtcgta gtcgacgcgc gagccgcggt accagagaca
      361 ggcgttgatc gcgctgcagc cgcccactac gcgggcgcgc ggcagcgcca gctttcgccc
      421 agccccgtcg gcgcggttgg tatagcccca ggagtgcgac tcgacctcgg agtggtaggg
      481 ctccagcagc tcggcgggcc aggcggcgag gtcggggccg tagtccggcc cggcgtcgag
      541 cagcagcacg cgccggccgg gatcctcgct cagccgcgcg gccagcacgc agcccgctgg
      601 ccccgccccg acgatgacga catcgaaccg ctcctgcatg gcgaaccccc actccaagac
      661 tgcccagtcg cctcgtccag tgtacggcga gccggccggc gcgtgaactc cgggagcgga
      721 accgcgcggc gctcagaacc agccgcgctg gcggaaggcc aagaggatca ccccgctgat
      781 cagcacgatg agggcgagca tgaccaggaa ggccgtcgag gtatagaggg gcacgccctg
      841 gaagttggtg ccgaagaccc ccaggatgac cgtggccggc aggagcacgg tcgagaccag
      901 cgtcaggatc ttcatcacct ggttggtgcg gtgcgacatc tgggagacgt agatgtcgaa
      961 ggcgccgttg accgcctccc tggtggcccg gatcttgtcg agcagccgcg ccagacgcgc
     1021 gtccaggtca cggaagtagg gctccacctc gccgccggag acgaagctga agtcgggacg
     1081 caggaacgcg gcgaagagct cctcgtgatg gtccagcaag cggctgacgg cgaagctgta
     1141 gcgcttgagg cgcagcagat catccaggaa ggtgtcgctc gtatccttga gcgcccgctc
     1201 ctccatctgc tcgatctcgt tctccagccc ctcgctcagg ccctcgccgt aggcgagcag
     1261 ctcgtcgagc accaggtaga gcatgaaggc ggcgtcgagc gaggccagct cggggcgctg
     1321 gcgggcgcgc gccagcaccc gtgccatgaa ccgcaggggg cgcttgtggg cgctgaccag
     1381 gaagttgtgg ccgatgaaga ggtcgagctc gctcgcctcg acccggtagg cggtggagtc
     1441 gcgggacggg accgtcaccg cgacgaagaa ctggtggtcg aagacatcca ggcggggccg
     1501 ttgccagacg gacagggcgg tgtggacggc cagcgtcggg agaccgagca gctcggccag
     1561 gtgtcgaagc tccgactggc tgtactccga gagatcgatc cagacgaaat tcgggtcttg
     1621 cgtgacgagg tcggggagat cagctacggc gacctggcgg gcctcctgct gctcgcggaa
     1681 cagccaggcc gtcacatccg tccgctcggc ctggggcgcg gaccagggcg cggcctcgcc
     1741 ctcaacccgc actgcgtccg ccactgggcc ggtatcgttc tggtctaccg cctgcacggg
     1801 agcctttacc accccgccgg ctacggtcgc ccccgcgccg tccttatcct gtggcgacgg
     1861 tactggccgg tcggcctgaa acgcttcgtg ctcctccatt gagatattct ccggttcacc
     1921 gcacactcgt catcgcgtgg tgccacgctc ttccgcggac agcccgcggc ctatctagcc
     1981 gcgaaccggg ccgcgagccg ccgcaggcga catgccaggg gaccgggtta tacggcgagg
     2041 gacaacgcct acatcagccc gccagcacgg ccaggaggta gcgttcgtcg cggcgcgcgg
     2101 tcaggcggcg cgccgtgacc ccgccccggc ccgtgcgctc ctccctgagc aggcgcaacg
     2161 cgatgcggcg cagttgcgcc agattccggc cggcgtgccc aaagggcagg ttcgtctccg
     2221 cgccgaaatc gatgtcgagc cgccagcttg gcccgcgcgg ctcctggcgt ggcgggggca
     2281 cggcgtcggc caggcgccgc gcgttgccct ccaggctgct cagatagtag cgctgctccc
     2341 ggttgacgac cccgccgcta tcgatctccc gctcgactag accgatgctg ttgagccgcg
     2401 gccagtgggc gcgctcgttg agcggcgcga ggtaggtggg gtcggtgatc agccatgctc
     2461 gccgtcgctc gccgccgtcg tgcccgcttg gctcggtctc atcatagtcg tgtgcgacgc
     2521 tggcgaagcc gccggtctcg gtctggctga acacggcagc gatcccgctc gtcagccgct
     2581 ccgcgttccc gttgagggtg acgacgtagt cggcgccctg gctgaccagg cgacgcgcga
     2641 tggtgcgctg gccggtggcc acatcgatcg tcacgacgct cttgccgggc gagagaaccg
     2701 ccagcgcctc cggcagggcg ctctcggcgg catcggccgc gcgctggctg aactggaggt
     2761 gtcg
//
LOCUS       DASUBI010000416         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1199390, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000416 DASUBI010000000
VERSION     DASUBI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199390"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..751)
                     /locus_tag="VFU72_08025"
     CDS             complement(14..751)
                     /locus_tag="VFU72_08025"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5423469.1"
                     /translation="MMVEERVAIVTGGARGIGRAIAAALLASGHRVGLADSDPGAAEQ
                     TAAAFGPAALAVSCDVADSAAVARMVDTVLGAWGRIDVLVNNAGITGQSKPLPEVSDA
                     EWLRVLGTNLNGAFYCCRAVVPHMLAGGWGRIVNIASIAGKEGNPNMAAYSASKAGLI
                     GLTKSLGKELAGTPIRVNCVTPAVIATDMMTAASPEMLAYMISRIPQGRVGQPEEVAA
                     LVAFLASDACSFSSGAVFDISGGRATY"
     gene            1025..2308
                     /locus_tag="VFU72_08030"
     CDS             1025..2308
                     /locus_tag="VFU72_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423470.1"
                     /translation="MTIAKPANEPPVIVSDKRARRAPLLALLAAMVVSEVGSRFTMIA
                     LPWFVLQTSGSAALTGLAGFAVALPGFLAGIFGGTLVDRLGFRRSSVVADAVSGAAIA
                     LIPALYFSVGLPFALLLALIFLGSLLEIPGVTARRAMLPELAESAGVRLERVNAAFES
                     TTHLSLLLGPPLAGLLIVWWGAATVLWIDAASFIVSALLVALLVPSLWAATPGPAGER
                     YRDAVRDGIRFLLHEPLLRAMAAGLAVSNATMAPLFAVVLPVYAQRDLGSATDLGLML
                     TTLGVGSLIGALGFGALAPSLSRRLVWFSGYLLAPVVYWVLLTEPGLVALLAAQFVVG
                     LATGPLNPLMVTIRHELIPAELRGRVFATYSAISQLVTPLGIVVAGFAIAGWGLHPAA
                     LALAVAAESVAVAMFFVPSLRHLPPAPAGVAREQT"
     gene            complement(2323..3147)
                     /locus_tag="VFU72_08035"
     CDS             complement(2323..3147)
                     /locus_tag="VFU72_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423471.1"
                     /translation="MALSTAEIMELALEMAGMSRVPGDSAVYVDGEELRRVLFGIDIG
                     PGELLLARQLGCDGVIAHHPAGGSATLNFPEVLTRQVELMVEHGVPVETARDAIQPLL
                     TRALLRAQTENFDRVPSVARRLELPFLNVHLPLDEVGRRMIVRRVEEHLTTLDREPLV
                     QDAIDALHEMPEFANAPTRIMVPVGAIDRPLGKLAVVHGAGSNGGAAVARAYFDSGVG
                     TVLYIHVAPEQAEILRREGRGNLIVSGHIASDLVGINPFVSALESRGVAVLRVSGL"
     gene            3264..3821
                     /locus_tag="VFU72_08040"
     CDS             3264..3821
                     /locus_tag="VFU72_08040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5423472.1"
                     /translation="MAVALIYETHSISHDNETGHATGWLPGELSPRGRDLARELGRRR
                     RDDPLAAVFVSDLRRAIETAELAFAGRDLPIIQDARLRECDYGALNGMPVARLAAERA
                     GHIDVPFPGGQSYRQVVTQTRAFLRDLAAGYDGATVLIIAHSANRWALDHLLQGVPLE
                     DLVDAPFHWQEGWRYTLPDGWDGVA"
     gene            complement(3877..>4422)
                     /locus_tag="VFU72_08045"
     CDS             complement(3877..>4422)
                     /locus_tag="VFU72_08045"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEU5423473.1"
                     /translation="TPPWWPDVPPQPKTQPATLELRAGDGALLASQAITVLDPSDPGV
                     QSIQLFWTVSGGDVGVVAETRAIPRTPQIGAAALRELLWGPPPVSQIGYSTALPLPAA
                     VLAFPGREPDWGSRVELLGLTIEDGVATANFSKELAAYGGGSTRVMLIRKQITQTLLQ
                     FPSIHEVRIAIAGQTEGVLEP"
BASE COUNT          590 a         1633 c         1508 g          691 t
ORIGIN      
        1 ctggggccgc cgctcagtag gtcgcgcgcc cgcccgagat gtcgaagacc gcgccgctgc
       61 tgaaggagca ggcgtcggag gcgagaaagg cgaccagcgc cgccacctct tccggctggc
      121 cgacgcgccc ttgcgggatg cggctgatca tataggccag catctcgggg ctggccgcgg
      181 tcatcatgtc ggtggcgatc accgccgggg tgacgcagtt cacccgaatc ggagtgccgg
      241 ccagctcctt gcccagcgac ttggtcaacc cgatcagccc cgccttgctg gcgctgtagg
      301 ccgccatgtt ggggttcccc tccttgccgg cgatcgaggc gatgttcacg atgcgccccc
      361 aaccgccggc cagcatgtgc ggcaccaccg cgcggcagca atagaaggcg ccgttgaggt
      421 tggtgcccag cacgcgcagc cactcggcgt cggagacctc gggtagcggt ttgctctggc
      481 cggtgatgcc ggcgttgttg accagcacgt cgatccggcc ccaggcgccc agcaccgtat
      541 cgaccatgcg cgcgacggcg gccgagtcgg ccacgtcgca gctgacggcg agcgcggcag
      601 ggccgaacgc ggccgccgtc tgctcggccg cgcccggatc gctatcggcc aacccgaccc
      661 ggtgcccgct ggccagcagc gcggccgcga tggcccgtcc gatcccgcgc gcccctccgg
      721 tcacgatcgc cacgcgctcc tcgaccatca tctcgcttcc tttccgccgc cacccggcgc
      781 gctcgcgcgc gagcgcgccc gacccctgat agtatcgcgc gcgctgccga atggactccc
      841 accggcgccg gggccggcga tcgctcgccc gagcagcgcc gggcacggcc gacacatcct
      901 actcagagat ttggtcaatt cacttactgt ctttgagtta gctcttgccg ttagcaagga
      961 atcggagtag actggtgtcg ctcgactggt tgactcctgc ggccgggcgg tttggagggg
     1021 accgatgaca atcgccaaac ccgcgaacga gcccccggtc atagtcagcg acaagcgagc
     1081 ccggcgcgca cccctactgg cgctgctggc ggcgatggtg gtgtccgagg tcggcagccg
     1141 cttcaccatg atcgccctgc cctggttcgt gctgcagacc agcggcagcg cggcgctcac
     1201 cggtttggcg gggttcgccg tggcgctgcc gggtttcctg gcggggatct tcggcggcac
     1261 gctggtcgac cggctcggct tccgccgctc cagcgtcgtc gcggacgcgg tcagcggcgc
     1321 ggccatcgcg ctgatcccgg cgctctactt cagtgtcggc ctcccgttcg cgctcttgct
     1381 ggccctgatc ttcctcggct cgctgctgga gatccccggc gtcaccgcgc ggcgcgcgat
     1441 gttgccggag ctggccgaga gcgcgggcgt acgcctggag cgcgtcaatg ccgccttcga
     1501 gtcgacgact cacctctcac tcctcctcgg gccgccgctg gccggcctgc tcatcgtctg
     1561 gtggggagcc gcgaccgtcc tctggatcga cgcggcgagc ttcatcgtgt ccgcgctgct
     1621 ggtggcgctc ctggtgccct cgctctgggc cgcgacgccc gggccggctg gagagcgcta
     1681 ccgcgacgcc gtgcgcgatg gaatccggtt cctcctccat gagccactgc tgcgcgccat
     1741 ggcggccggg ctggcggtca gcaacgccac catggcgccg ctcttcgcgg tggtcctgcc
     1801 ggtctatgcg caacgcgacc tgggcagcgc caccgatctg ggactgatgc tgaccacgct
     1861 cggagtcggc agcctcatcg gcgcactcgg cttcggcgcg ctggcgccct cgctctcccg
     1921 gcgtctggtc tggtttagcg gctatctgtt ggccccggtc gtctactggg tcctgctcac
     1981 ggaaccgggg ctggtggcac tgctggcggc ccaattcgtg gtcgggctgg cgaccggccc
     2041 gctcaacccg ctcatggtca cgatccgcca cgagctgatc ccggccgagc tgcgcgggcg
     2101 cgtcttcgcg acctactcgg ccatcagcca gctcgtgacg ccgcttggga tcgtcgtggc
     2161 cgggttcgcc atcgccggct ggggactgca tccggcggcg ctggcgctcg ccgtcgcggc
     2221 cgagagcgtc gccgtggcga tgttcttcgt gccctcgctg cggcacctgc cgcccgcccc
     2281 ggccggggtt gcccgcgaac agacttgagg cgccagccgc gcctacagcc cgctgacccg
     2341 cagcacggcc accccgcgac tctccagcgc ggagacgaag gggttgatgc cgaccaggtc
     2401 gctggcgatg tggccgctga cgatcaggtt gccccggccc tcgcggcgca ggatctccgc
     2461 ctgctccggc gccacatgga tgtagagcac ggtgcccaca ccggagtcga agtaggcgcg
     2521 cgccaccgcc gcgccgccgt tgctgccggc gccgtgcacc accgccagct tgcccagggg
     2581 gcggtcgatc gcccccacgg gcaccatgat gcgcgtgggc gcgttggcga actccggcat
     2641 ctcatgcagc gcgtcgatcg cgtcctgcac cagcggctcg cggtcgagcg tggtcaggtg
     2701 ctcctcgacc cgccgcacga tcatgcgccg gccgacctca tccagcggta ggtggacgtt
     2761 caggaagggc agctccaggc ggcgggccac cgagggcacc cggtcgaagt tctcggtctg
     2821 cgcgcgcaga agcgcgcgcg tcagcagcgg ctggatcgcg tcgcgcgcgg tctcgaccgg
     2881 cacgccgtgc tcgaccatca gctcgacctg gcgcgtcaac acctcgggga agttgagggt
     2941 ggcgctgccc ccggcggggt ggtgcgcgat caccccatca cagccgagct gcctggccag
     3001 tagcaactcg ccggggccga tgtcgatgcc gaagagcacg cgccgcagct cttcgccgtc
     3061 gacgtagacg gcgctatcgc cgggcacgcg cgacatgccg gccatctcca gcgccagctc
     3121 catgatctct gccgtgctca acgccatagc cgctcctcga cttttcacct tcgcaccgcg
     3181 ccggccgcga tttgcgccga cctggcatgt ccgccacaat caggcactag cgtacccgaa
     3241 gtcgggccag gcggaggagc cggatggcgg tcgcgctcat ctacgagaca cactcgatca
     3301 gccacgacaa cgagaccggc catgccaccg gctggttgcc gggcgagctc tcgccgcgcg
     3361 gccgcgatct agcgcgcgag ctgggccggc gccggcgcga tgatccgctg gcggcggtct
     3421 tcgtctccga cctgcgccgc gcgatcgaga ccgccgagct ggcgttcgcg ggccgcgacc
     3481 tcccgatcat ccaggacgcg cggctgcgcg agtgcgacta cggcgcgctc aacggcatgc
     3541 cggtggcacg actggcggcc gagcgcgccg ggcacatcga cgtgcccttc cccggcggcc
     3601 agagctaccg ccaggtcgtg acgcagacgc gcgccttcct gcgcgacctg gccgccggtt
     3661 atgacggcgc cacggtcctg atcatcgcgc actccgccaa ccgctgggcg ctcgaccacc
     3721 tgctgcaggg cgtgccgctg gaagatctgg tcgacgcccc cttccactgg caagagggct
     3781 ggcgctacac gctgccggac ggctgggatg gtgttgcatg agcgactcgc ttgcgcgcga
     3841 tgaagtcatc atcaccgggc cgcgcacgct ggccgcctac ggctccagca cgccctcggt
     3901 ctgccccgcg atggcgatgc ggacctcgtg gatgctcggg aattggagca gtgtctgggt
     3961 gatctgcttc cggatgagca tcacccgcgt tgagccgccg ccgtaggccg ccagctcctt
     4021 ggagaagttg gccgtcgcca caccgtcctc gatggtcagt cccagcagct cgacgcgtga
     4081 accccaatcc ggctcgcgac cggggaacgc cagcaccgcc gcgggcagcg gcaacgccgt
     4141 gctgtagccg atctggctca ccggcggtgg accccagagc aactcccgca gcgcggccgc
     4201 gccgatctgc ggcgtacgcg ggatcgcccg cgtctccgcg acgacgccaa catcaccgcc
     4261 cgataccgtc cagaagagct gaatggactg cacgccggga tcgctggggt cgagcacggt
     4321 gatcgcctgc gaggcgagca gcgcgccgtc gccagcacgc agctcgagcg tcgccggctg
     4381 cgttttgggc tgcggtggca cgtccggcca ccagggcggc gt
//
LOCUS       DASUBI010000417         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_59200, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000417 DASUBI010000000
VERSION     DASUBI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59200"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VFU72_08050"
     CDS             complement(<1..2154)
                     /locus_tag="VFU72_08050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5423474.1"
                     /translation="MDKAKTDLGTRSRELSRKYEPARVEEHWYDWWDSQGYFQAPIVP
                     GKQPFVIIMPPPNVTGSLHMGHALFVTVEDILARWHRMLGDPTLWLPGADHAGIAGQW
                     VVERELAAEGLTRHDLGRDKFLERVWDWMDRYRGRIREQMRLLGASCDWSHFVFTMDP
                     APSRAVRTAFKRLYDKGLIYQGERLINWCPRCMTALSDLEVNHEEVHSSLWTLRYPIV
                     GTGAEEESQRFIEVATTRPETMLGDTAVAVNPDDARYRDLVGLQVHLPLLGRIIPIVA
                     DPAVDPVFGTGAVKVTPAHDPNDFEIGQRHNLSAITVINHDGTMNAEAGPFVGMTVDE
                     ARKAVVRQLELEGYLAGTQPHTHAVGTCERCGTVVQPLISRQWFLTMKPLAEPAVEVV
                     KDGTVRFIPERFTGIYLNWMENIHDWCISRQLWWGHRIPVWYCRDCGEVIVTDQESVE
                     RCACGSTNIYQDPDVLDTWFSSGLWTFSTLGWPDDTEELAYFYPGTVMETGYEIIFFW
                     VARMIFFGLEFMGEVPFRDVYLHGTVRDEFGQKMSKTKGNVIDPIDVTQQYGSDALRF
                     ALITAGGAGTDSKLSLSRVEAARNFGTKLWNATRYVLRALESGAVAVDESGAPLPPDF
                     ATAGLADRWIVSRLQQVSDEVQDLMLAFKFDESSRRIYDFFWSEFADWYIEASKPAIN
                     GGDAVAAAGARQTLAYVLERALRLLQPVMPFVTEEL"
     gene            complement(2555..>2995)
                     /locus_tag="VFU72_08055"
     CDS             complement(2555..>2995)
                     /locus_tag="VFU72_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5423475.1"
                     /translation="LEAVAAVDGAVLDPRFFMYCEDVDLSWRLRLRGYAIVYAPEALV
                     YHRLSATGGGPLASYYVARNTLAVLIKDVPSPILRRNLARIVLAQLSGALVTVPHLGE
                     PAARARLRGLAAFPRLLPTLLRQRRRIQASRVVSAETLGRLLEP"
BASE COUNT          480 a         1018 c         1027 g          470 t
ORIGIN      
        1 cagctcctcg gtcacgaacg gcatcaccgg ctggagcagg cgcagcgcac gctccagcac
       61 gtaggccagt gtctgccgcg cgccggccgc ggccaccgca tcgccgccgt tgatcgccgg
      121 cttgctggct tcgatgtacc agtcggcgaa ctccgaccag aagaagtcat agatccggcg
      181 cgacgattcg tcgaatttga aggccagcat caagtcctgc acctcgtcgc tgacctgctg
      241 taggcggctg acgatccagc ggtcggccag cccggcggtg gcgaagtctg gcggcagcgg
      301 cgcgccgctc tcgtcgacgg ccaccgcgcc cgactcgagc gcgcgcagca cgtagcgggt
      361 ggcgttccag agcttggtgc cgaagttgcg cgccgcttcc acgcgcgaca ggctcagctt
      421 ggagtccgtg ccggcgccgc cggccgtgat cagcgcgaag cgcagcgcgt cgctgccgta
      481 ctgttgggtg acgtcgatcg ggtcgatcac gttccccttg gtcttgctca tcttttgccc
      541 gaattcatcg cgcaccgtgc cgtgcaggta gacgtcgcgg aacggcacct cacccatgaa
      601 ctccaacccg aagaagatca tgcgcgccac ccagaagaag atgatctcgt agccggtctc
      661 catcaccgtg ccggggtaga agtaggccaa ctcctcggtg tcgtctggcc agccgagcgt
      721 gctgaaggtc cagaggcccg agctgaacca ggtgtcgagc acgtcggggt cctgatagat
      781 gttcgtgctg ccgcaggcgc agcgctcgac cgactcctgg tcggtgacga tcacctcacc
      841 gcagtcgcgg cagtaccaga cggggatgcg gtggccccac cagagctggc gcgagatgca
      901 ccagtcgtgg atgttctcca tccagttgag gtagatgccg gtgaagcgct ccggaatgaa
      961 gcgcaccgtg ccgtctttga ccacctcgac cgccggctcg gccagcggct tcatcgtcag
     1021 gaaccactgg cggctgatca gcggctggac caccgtgccg cagcgctcgc aggttccgac
     1081 ggcgtgagtg tgcggctgcg tccccgccag gtagccctcc agctcgagct ggcgcaccac
     1141 ggccttgcgc gcctcgtcga cggtcatgcc gacgaagggc ccggcttcgg cgttcatggt
     1201 gccgtcgtgg ttgatgacgg tgatggccga caggttgtgg cgctggccga tctcgaagtc
     1261 gttcgggtcg tgcgccggcg tcaccttgac cgcgccggtg ccgaagacgg ggtcgaccgc
     1321 gggatcggcc acgatcggaa tgatgcggcc cagcagcggt aggtggacct gcagcccgac
     1381 cagatcgcgg tagcgcgcgt cgtcggggtt gaccgccacc gccgtgtcgc cgagcatcgt
     1441 ctccggccgg gtggtggcca cctcgatgaa gcgttggctc tcttcctcgg cgccggttcc
     1501 tacgatcggg tagcgcaggg tccagagcga ggagtggacc tcctcgtggt tgacctccag
     1561 gtcggagagc gcggtcatgc agcgcgggca ccagttgatc aggcgctcgc cttggtagat
     1621 cagccccttg tcgtagagcc gcttgaaggc ggtgcgcacc gcgcgcgagg gggccgggtc
     1681 catggtgaag acgaagtgcg accagtcgca ggaagcgccg agcaggcgca tctgctcgcg
     1741 gatgcggcca cggtagcggt ccatccagtc ccagacgcgc tccaggaact tgtcacgccc
     1801 gagatcgtgg cgggtcagcc cctcggcggc caactcgcgc tcgaccaccc attgtccggc
     1861 gatgccggcg tggtcggcgc cggggagcca gagggttggg tcgcccagca tacggtgcca
     1921 gcgggccagg atatcctcga cggtgacgaa gagggcgtgg cccatgtgca aggaaccggt
     1981 gacgttgggc ggcggcatga tgatcacgaa gggctgcttg ccgggcacga tcggcgcctg
     2041 aaaatagccc tgggaatccc accagtcgta ccagtgctcc tcgacccgcg ccggctcgta
     2101 cttgcggctc agctcccgcg atctcgtgcc gagatccgtc tttgccttgt ccatcctcgg
     2161 cctgaacctt ccgccgggct gcccccggtc acgcctgtcg ccgcagctcc caagccgagc
     2221 acgatccgcg ccgccggcaa caaaaaaacc gtcctcactc aaggacggct gacaccgcgg
     2281 taccaccttg cttcggcagc agacgctgcc gcgctcgaac gcgctaacgg gcgtacccgc
     2341 gacggcctac gcactgcgat gcagcttcgg ccgccggcct cccggatgac ttcgatcgct
     2401 ctccaccggg cggggctctc aaccggtgac cccgcgtctc tgtcggcgag cgcgcgatct
     2461 actccctccg atcgccggca ctcgactttc cctctatttt acgcagcgcg tcaagagggt
     2521 ggcggtggcc gtgcagcccg cacgcgacag acggctatgg ctccagcagc cgccccagcg
     2581 tctccgccga cacgacgcgg cttgcctgga tacggcggcg ctggcgcagg agcgtgggca
     2641 gcaggcgcgg gaaggcggcc aggccacgca gccgggcgcg ggcggcgggc tcgcccaggt
     2701 ggggcacggt caccagcgcg ccggagagct gcgccaggac gatgcgcgcc aggttgcgcc
     2761 tcagaatcgg ggaagggaca tccttgataa gcaccgccag cgtgttgcgc gcgacataat
     2821 acgaagcgag cggcccgccg ccggtcgccg agagccggtg gtagaccagg gcctccggcg
     2881 cgtagacgat cgcgtagccg cgcaggcgca ggcgccagga gagatcgacg tcctcgcagt
     2941 acatgaagaa gcgggggtcg agcaccgcgc cgtcgacggc cgcgacggct tcgag
//
LOCUS       DASUBI010000418         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_398736, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000418 DASUBI010000000
VERSION     DASUBI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398736"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFU72_08060"
     CDS             complement(<1..1154)
                     /locus_tag="VFU72_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU5423476.1"
                     /translation="MQLPISFVFEPPTRADLRDAARNPRLWVLAAALCLLVAGAIILA
                     RAQRAARELPADAALTVTSTPAGAAVVIDGRERGSTPAALALPRGHHWVTLRLARYGD
                     VSYEVDLAPDRPATLDGILWTPTPLVQRLRSPLPGAAIADARFLADGRLALILALPGR
                     DERQLWLVDRGGDARLFGPPGARLALTIARDGDRVAYLARGTAPASGTPTIDPTGAPR
                     LDEVWVAGLDGERGERRYALPPNTADERLVDLSWSPDGGHLLLVSQQRPQGGTLRTRL
                     LRLDTGTGEARELIALPGEVVPASYEWSPAGDRVALLIRAASRTTLCLVGTADGSFRA
                     LAEAGSTPFPPLAWSPRGDRLAYSAQPDAPTADPSGNSALVAIHTDDLDGG"
     gene            1936..>2340
                     /locus_tag="VFU72_08065"
     CDS             1936..>2340
                     /locus_tag="VFU72_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423477.1"
                     /translation="MVATTEQLWHGLHDRLLRSIRRHVPDAASAEDILQDVFLKIHLR
                     IGTLRDEERVEGWVYQIVRNAITDHRRRQRPSGPLPEELAASEAADEDDAGSVARQLI
                     PFIKATVAGLPEPYREALLLTEYEGLTQQQLAE"
BASE COUNT          365 a          772 c          885 g          318 t
ORIGIN      
        1 ccgccgtcga gatcgtcggt gtggatcgcg acgagcgcgg agttccccga cgggtcggcg
       61 gtaggcgcgt ccggctgagc ggaataggcg agccggtcgc cgcgcggcga ccaggcgagg
      121 ggtgggaatg gtgtactccc agcttcggcg agggcgcgga acgacccatc ggcggtgccg
      181 acgaggcaga gggtcgtgcg gctcgcggca cggatgagca gggccacccg atcgcccgcc
      241 gggctccatt cgtatgaggc ggggacaacc tcgcccggca gggcgatgag ctcgcgcgcc
      301 tccccggtac cagtgtcgag gcggaggagg cgcgtgcgga gggtgccgcc ctggggccgc
      361 tgctggctga cgaggagcag gtgcccgccg tcgggtgacc agctcagatc gacgagccgc
      421 tcatcggcgg tgttcggtgg cagcgcgtac cggcgctcgc ctcgctcgcc gtcgaggccg
      481 gccacccaga cctcgtcgag gcgcggggca ccggtgggat cgatcgtcgg ggtgccgctg
      541 gccggggcgg tgccgcgcgc gaggtaggcg acccggtcgc catcgcgcgc aattgtcagc
      601 gcgagccgcg cgccgggcgg gccgaacagg cgggcgtcgc cgccccggtc gacgagccag
      661 agctggcgct cgtcgcggcc cggcaaggcg aggatcagcg cgaggcggcc atcggcgagg
      721 aagcgggcgt cggcgatcgc ggccccaggg agcggcgacc ggagccgctg gacgagcggc
      781 gtcggggtcc agaggatgcc gtcgagcgtc gccgggcggt ccggcgcgag gtcgacttca
      841 taggaaacgt ccccgtagcg cgcgagccgg agcgtgaccc agtggtgacc gcgcggtagc
      901 gccagcgcgg cgggcgtact gccccgttcc cgcccatcga tcacgaccgc cgcgccggcg
      961 ggtgtgctgg tgaccgtcag cgcggcgtcc gccgggagtt cgcgcgccgc cctctgggcg
     1021 cgcgccagga tgatcgcccc ggcgacgagc aagcataggg ccgccgccag cacccatagg
     1081 cgcgggttcc gggccgcgtc gcgcaggtcg gcgcgcgtcg gtggctcgaa gacgaaggaa
     1141 atgggaagct gcatggtcgg ccctcctcgt gtgagtccgt cgctacggac cacgctacga
     1201 gggccggctg cccctacacc gcccggtggc tgccccaaga ccgccccgaa cgaatctgcc
     1261 agctaatggt gatcctaccg aacgccgttg gcgagttcat gctctatggg tatgcagagc
     1321 gcaccgacgg cggggcatgg agttcggagt cggtcttgga gacaagtggc ttcgcgcctg
     1381 attcgctcat acggctgatc gatgatcttg cgccattcgc cccaactgca ggtgactggt
     1441 gcgccgaata acggccaccg ggagctaaga gagggctgcg tcctttcctc agcagttgcg
     1501 tctctattgg tacgagcggc cgatgggtgc cgcatgaaag agacagcagg gagtcggaca
     1561 tgacaaccat ggcgatggac acggcgacgg tgcggagtgt tccggcggac gagcagcgcc
     1621 caatagagga tcggccgacg gcacaggcgt cggcgtgttg cggcgcgacc gagcaggcga
     1681 cctgctgcgc gccgtcggtg aaggtagcct gctgtgggac gccggccgcc gatggcgcgg
     1741 caccgtgcgg ctgccggtga gcaggagaag gtgacctctc ccctgcggct ccgcgccagc
     1801 gtggccatcg gcatcgcgcc gggcagcgcg cgggggagca gtgttccctc ccggcacgtt
     1861 cttccttata ctgctgatca aagcggctga tcaccgctga cgaaagtcac agcacgcgga
     1921 atcacgaagg tgagcatggt tgcgacgaca gaacagctct ggcacggcct ccacgaccgg
     1981 ctgttgcgct cgatccgccg gcacgtccct gacgcggcga gcgccgagga catcctgcag
     2041 gatgtcttcc tcaagatcca cctgcggatc ggcacgctgc gcgacgagga gcgggtggag
     2101 ggctgggtct accagatcgt ccgcaatgcc atcaccgacc accggcgccg ccagcggccc
     2161 agtggcccgt tacccgagga attggccgcg tcagaagcag cggacgagga cgacgcgggc
     2221 agtgttgccc ggcagctcat ccccttcatc aaggcgacgg tcgccggcct gcccgagccg
     2281 taccgcgagg cgctgctgct gaccgagtac gaggggctga cccagcagca gctcgccgag
//
LOCUS       DASUBI010000419         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1013978, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000419 DASUBI010000000
VERSION     DASUBI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013978"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1605)
                     /locus_tag="VFU72_08070"
     CDS             complement(214..1605)
                     /locus_tag="VFU72_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU5423478.1"
                     /translation="MYDAELMADYALGLRFEELPAEVVAAAIVAVADTLACAVGALDE
                     PGPAILRDYAREQGGKPAATLVGGQARVPAPLAALVNGALARDLDANDLYATPPGRDT
                     GHFSDAIPAVLAASEESGASGRDFLTAVVVAYELQAVLAEAYLWMQRGLHSVSQVAWA
                     VPAAAGRLRGLARDEIVAAIGLAGTTGGLILQSWLRPSTTLPLIKGGAPGFAGQRALE
                     ATALAARGFSAPSDALETLFERLPSEVDAAPFGRLLRRDVFTTPRNMLKRWPAQIYTQ
                     AAIQAAVALHPAIGEVDRIAVATLYGHRQVCAGVQGSAAAYTPQTRGAADHSTPFVVA
                     IALRDGDLTPASYAGEPWHDPALLELMRRVDLVIDPTFEEALVEAGRFGCRLVVELLD
                     GQRLEATVRQQNGHPEARLSRDELLAKLRVFVDPKLGPGAAARLLATAEALPESPDVS
                     ALLAACAGSAGTG"
     gene            1736..2491
                     /locus_tag="VFU72_08075"
     CDS             1736..2491
                     /locus_tag="VFU72_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423479.1"
                     /translation="MGEAPGPVIEVRGVSQAFGAVRALEDVSLAIPPGVIYGLIGPSG
                     SGKTTLIRLLVGVLRPERGEVRVLGRPMPDRAVAGRIGYMTQSTALYPDLSLRENLAF
                     FGALYGLDNEQLRRRIDEVSREVALADRLDGPLHTFSGGMQRRASLACALIHQPELLI
                     LDEPTVGLDPVLRRAFWARFHQLAQAGRTLIVTSHIMDEAGRCDLLAFIRDGRLLASG
                     TPEALRRLTGQGELEEAFLALAEHEKLPAEETR"
BASE COUNT          368 a          985 c          951 g          356 t
ORIGIN      
        1 gccagcgtcc acgtgaactg cggccaccac ggcgatcgcg ctacccgtgt cacacattgc
       61 cgcgtggagc cgtcagagag atccttcggc tgcggcctca ggatgacaga gcaaacggcc
      121 tactattccc tgtcatcctg agccgcagcg aagggtctct catcggcaat gagcggcatc
      181 caccgtaaca agtggacgcg gaacgactcc acctcagccc gtgccagcgc tcccggcgca
      241 cgcggcgagc agcgcactga cgtccggaga ctcgggcagc gcctcggcgg tggctagcag
      301 gcgcgcggcg gcgccggggc cgagcttggg gtcgacgaag acgcgcagct tggccagcag
      361 ctcgtcgcgg ctgaggcgcg cctcggggtg gccgttctgc tggcgcaccg tcgcctccag
      421 ccgctgcccg tcgagcagct ccaccaccaa ccggcagcca aagcgccccg cctcgaccag
      481 cgcctcctcg aaggtggggt cgatcaccag gtcgacgcgg cgcatcagct cgagcagggc
      541 cgggtcgtgc cagggctcgc cggcgtagct ggccggggtc aggtcgccgt cgcgcagcgc
      601 gatcgcgacc acgaagggcg tgctgtggtc ggccgcgccg cgcgtctggg gcgtgtaggc
      661 cgccgccgag ccctgcaccc cggcgcagac ctgccggtgc ccgtagagag tggcgaccgc
      721 gatccgatcg acttcaccga tggcggggtg cagcgcgacc gccgcctgga tggccgcctg
      781 ggtgtagatc tgcgccggcc agcgcttgag catgttgcgg ggggtggtga agacgtcgcg
      841 ccgcagcagc cgtccaaagg gcgccgcatc gacctcggac ggcaggcgct cgaagagggt
      901 ctccagcgcg tcacttggcg cgctgaagcc gcgcgccgcc agcgccgtcg cctccagcgc
      961 gcgctgcccg gcgaagcccg gtgcgccccc cttgatcagc ggcagcgtgg tcgaggggcg
     1021 tagccacgac tgcaagatca gcccaccggt ggtgccggcc agcccgatgg ccgccacgat
     1081 ctcgtcacgc gcgaggccgc gcaggcgccc ggcggcggcg ggcaccgccc aggccacctg
     1141 gctgaccgag tgcagcccgc gctgcatcca gagataggcc tcggccagca ccgcctgcag
     1201 ctcgtaggcg acgacgaccg cggtcaggaa atcgcgcccg ctggcgccgg actcctcgga
     1261 ggcggccagc acggcgggga tggcgtcgga gaagtggcca gtgtcgcgcc cgggcggggt
     1321 ggcgtagaga tcgttggcgt cgaggtcgcg cgccagcgcg ccgttgacca acgccgccag
     1381 cggcgcgggc acgcgcgctt gaccgcccac cagcgtcgcc gccggcttcc ctccctgctc
     1441 ccgcgcgtag tcgcgcagga tggccggtcc gggctcgtcg agcgcaccca ccgcgcaggc
     1501 cagcgtgtcg gccacggcca cgatcgccgc cgccacgacc tccgccggca gctcttcgaa
     1561 ccgcaggccg agcgcgtagt cggccatcag ctccgcgtcg tacatcgcgc ctccttccgg
     1621 cgtcaattct agccagccgg gcggcgcgac tgccgactgc cgcagccggt ggtgtcgcta
     1681 gcgctatcct gctatcgtca gaggcgcgac cgcgagcgac cagacggagg agcggatggg
     1741 cgaagcgccg gggccggtca tcgaggtgcg cggggtgagc caggcgttcg gtgcggtgcg
     1801 ggcgctggag gacgtctcgc tcgccatccc gccgggcgtg atctacggcc tgatcggccc
     1861 tagcggctcg ggcaagacga cgctgattcg cctgctggtc ggcgtgctgc gccccgagcg
     1921 cggcgaggtc cgcgtcctcg gccgcccgat gcccgaccgc gccgtcgccg ggcgcatcgg
     1981 ctacatgacg cagtcgaccg cgctctaccc cgacctctcg ctgcgcgaga atctggcctt
     2041 cttcggcgcg ctctacgggc tggacaacga acaattgcgg cggcgcatcg acgaggtcag
     2101 ccgcgaggtg gcgctagccg accggctcga tggaccgctg cacaccttca gcggcggcat
     2161 gcagcggcgc gcctcgctcg cctgcgccct gatccaccag cccgagctgc tgatcctcga
     2221 cgagccgacc gtcgggctcg atccggtgct gcgtcgcgcc ttctgggccc gcttccatca
     2281 actcgcccag gccgggcgca cgctgatcgt caccagccat atcatggacg aggccgggcg
     2341 ctgcgacctg ctcgccttca tccgcgacgg ccgcctgctg gccagcggga cgccggaggc
     2401 actgcgacgg ctgaccggcc agggcgagct ggaggaagcc ttcctggcgc tggccgagca
     2461 cgagaagctg ccggcggagg agacgcgatg agccggcgcc gcgtgctggc gctcgccagg
     2521 cgcatcgtga agcagttccg gcacgaccgc cgcaccctct tcctgatgct ggtcgcccag
     2581 atcctggtcc tgtcgctgct ggcctacatc taccgcggcg gcgggcaccc cgtcgcgctg
     2641 gcggtggtcg atccggcccc
//
LOCUS       DASUBI010000420         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1202591, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000420 DASUBI010000000
VERSION     DASUBI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202591"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="VFU72_08080"
     CDS             <1..1905
                     /locus_tag="VFU72_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5423480.1"
                     /translation="EIGSLLATIETPSTPLQRLITRLIRQLALVALLVCLLVGLLELL
                     TGHGWAAAIIAAVSLAIAAIPEEFPMVYTLYLSLGAWRLARDHALVRRLTSVETLGST
                     TVICTDKTGTLTLGTVEVAGVAPVGSEVLTGQQPLTEPARALLEAAALASEPRPYDPL
                     DQSILRFAEAHGIDTTALHRATLLHDYPFDPVRKHVTHVWQGPTGIRCVAKGAFEGLI
                     EVTGSSGEDLREIRAANHRLAAQGMRVIAVASGNPANVTGVRGEDERGLRLAGLIAFS
                     DPLRPGVRDALRRCREAGIRVILITGDHPATAHAVADSLELPHQDVQIATGADIDAAD
                     DSELATLVRRVNIFARTRPEQKYRLVTALRSGGEVVAMTGDGTNDAPALREADIGVAM
                     GQRGTEVARAAAQLVLLDDNFATIVTAVRNGRRIFVNLQHAFAYLIAFHIPLLLTALL
                     VPLVGLPLMLLPMHLIWLELIVHPTASLVFEADDGQDDVMRQPPRRQQDLIPRRELVR
                     ATVAGLVLTAAVFGLYAALIWLGHEAAHARASALVALIVGQMTLTLTERAYPRPINRA
                     VFQSRTLLLVVAGVLATLFAGLYFPPAARILLLAPLGHTALLASIVVGLAATLWSQPL
                     ARSRTHHQPR"
     gene            complement(2007..>2517)
                     /locus_tag="VFU72_08085"
     CDS             complement(2007..>2517)
                     /locus_tag="VFU72_08085"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEU5423481.1"
                     /translation="PSPTGEAELDAALELIHGSDAVRLARHWVRQLGDQPGLTAQLAR
                     RLVARGILREQEHTFLWLFHSLRFPTSEPGREASVRDQIRALVLGSAAPDARTLMLLS
                     LIYACKLTDDVFSREERVRAARRIKDLVEHEQFGAAVGEAVADTLVAITAAVNAAVFT
                     VTVAPGASH"
BASE COUNT          380 a          898 c          816 g          423 t
ORIGIN      
        1 gagatcggca gcttgctcgc taccatcgag acgccatcga ctcccctgca acgcctgatc
       61 acacgcctca tccggcagtt ggcgctggtg gcgctgctgg tctgcctgct ggtagggctg
      121 ctcgaactcc tgaccgggca cggctgggcg gcggccatta tcgccgccgt cagcctggcg
      181 atcgccgcga tcccggaaga gtttccgatg gtctacacgc tctacctgtc cctgggcgcc
      241 tggcgtttgg cgcgcgacca cgcgctggtt cgtcgcctga ccagtgtcga gacgctggga
      301 tcgaccaccg tcatctgcac cgacaagacc gggacgctca ccctcgggac ggtcgaggtc
      361 gcgggagtgg cgccggttgg atctgaggtg ctcaccgggc agcagccact gacggaaccg
      421 gctcgcgccc tgcttgaagc cgcggccctg gcctcggaac cgcggcccta tgaccccctc
      481 gatcagtcga ttttgcgttt tgccgaggcg cacgggatcg acaccacggc cctccaccgc
      541 gcgacgttgt tgcacgatta cccgttcgat ccagtccgaa agcacgtcac ccacgtgtgg
      601 caggggccga ccgggattcg gtgcgtggcg aaaggggcgt tcgaagggtt gatcgaagtg
      661 accggcagtt ccggcgagga cttgcgggag atccgcgccg cgaaccatcg gctcgcggcc
      721 cagggcatgc gggtcatcgc cgtggccagc ggcaaccctg ccaacgtcac tggcgtgcgc
      781 ggtgaggacg agcgcggact ccggctggct ggcctgatcg cgttctccga cccgctgcgc
      841 cccggagtgc gagacgcgtt gcgccgctgc cgcgaggccg ggattcgcgt catcctgatc
      901 accggtgacc acccggcgac cgcccacgcg gtggccgaca gcctggagct gccgcatcag
      961 gatgtccaga tcgcgaccgg agccgatatc gacgccgccg acgactcgga gcttgccacg
     1021 ctggtgcggc gggtcaatat cttcgcccgc acccgtcccg agcagaaata ccgcctggtg
     1081 acggccctac gctcgggagg cgaggtggta gccatgaccg gcgacggcac gaatgacgcg
     1141 ccggccctgc gcgaggccga catcggcgtg gccatggggc aacgcggcac cgaggtcgcc
     1201 cgtgccgccg ctcaactcgt cctgctcgac gacaacttcg ccacgatcgt cacggcggtg
     1261 cggaacgggc gccggatctt cgtcaacctg cagcacgcct ttgcttacct gatcgcgttt
     1321 cacatccccc tcctcctgac ggccctgctt gttcccctgg tcggcctccc gctcatgctg
     1381 ctgccgatgc atctcatctg gttggagctg atcgtccatc ccacagcctc gttggtcttc
     1441 gaggcagacg atggccaaga cgatgtgatg cggcagcccc cgcgccggca gcaagacctc
     1501 attcctcgac gggagctggt gcgggccacg gtagccggcc tggtgttgac ggccgccgtg
     1561 tttggtctct atgccgcgct catctggctc ggccatgagg cggcccacgc gcgcgcctcg
     1621 gcgctagtcg ccttgatcgt cgggcagatg acgctcacgc tcaccgaacg cgcgtatccg
     1681 cggccaatca atcgcgcggt attccagagc aggacgctcc tcctggtcgt cgcgggagtg
     1741 ttggcgaccc tcttcgccgg cctctatttc ccgccggccg cccgaatcct cctgctggcg
     1801 ccgctggggc acacggcgct tctcgcctcg atcgtggtcg ggcttgccgc cactctgtgg
     1861 agccagccgc tcgcgcgctc gcgcacgcat caccagcctc gatgatgccc gcccgatgcg
     1921 tcgctaaacc acgttgcgcc tgccgcccaa taccgggcga caggcgaagc tggcggcgac
     1981 agtgcttgga ctacagtgct gaagcctcag tgggaggctc cgggggcgac cgtgaccgtg
     2041 aacaccgccg cgttgacggc tgccgtaatc gccaccagtg tgtccgccac cgcttcaccc
     2101 acggccgcgc caaactgctc atgctcgacc agatccttga tgcgccgcgc cgcccgcaca
     2161 cgctcctcgc gcgagaacac gtcgtcggta agcttgcagg catagatcag gctcagtagc
     2221 atcagcgtcc gcgcgtccgg cgcggcactg ccgagaacca gcgcgcgaat ctggtcgcga
     2281 accgaggctt cgcggccggg ctcgctggtg ggaaagcgca gtgagtggaa gagccacagg
     2341 aacgtgtgct cctgctcgcg cagaatgcca cgcgccacta gccgccgcgc cagttgtgct
     2401 gtcagccccg gttgatcgcc gagttgtctc acccagtgcc tggccaagcg cacggcgtcg
     2461 gagccgtgga tcagctccaa cgcggcatcg agctccgcct cgcccgtcgg cgacgga
//
LOCUS       DASUBI010000421         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_45717, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000421 DASUBI010000000
VERSION     DASUBI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45717"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..869)
                     /gene="istB"
                     /locus_tag="VFU72_08090"
     CDS             complement(72..869)
                     /gene="istB"
                     /locus_tag="VFU72_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEU5423482.1"
                     /translation="MSDQPTGLQGRIVQQQCKQLGLPTVFAQCLPLAEQAQREQHPHL
                     DYLAALFAAELEDREQRAVARRLQEAHLPRIKTLDEFDFAQTPAVSAAQLHQLAEGGY
                     IERAEPVVFLGDCGTGKTHLLTGLCVAACRQKRRVRFTTAAALVTELVEAKQQLQLGR
                     VLGRWARYDVIAIDEVGYVPLAEVGAEFLFQIIAERAEKAAVLLTTNLPFSEWTQVIP
                     NARLCKALLDRITDRAHIIETGTDSFRFRRTLKQRNHGSKGKEAAAE"
     gene            complement(866..>2175)
                     /gene="istA"
                     /locus_tag="VFU72_08095"
     CDS             complement(866..>2175)
                     /gene="istA"
                     /locus_tag="VFU72_08095"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEU5423483.1"
                     /translation="TRLREERPGLVIGESTVRQYVRERKRTLGLLARETCVPQEYAWG
                     EEAQVDWYEAVAVVDGTPQTWQVFSMRSMASGAAFHRAYPRATQQAFLEAHELAFAYF
                     GGVFRRLRYDNLGAAVQKVLRGHRREETTRFVGFRSHWRYAAEFCTPGEGHEKGGIEN
                     EAGTYRRNHWVPIPHVADLAALNAHLLAGCRADEARVLAGRERPVGVALLLEREHLLP
                     LADEGFDLAETSFATVDTLGRVRVRTNAYSVPLPPGTQVQIKVLPATVELWHGGKRVA
                     AHERCYGRGREILDLEHYLDVLEHKPGALAGSKPLAEWRRLGRWPESYDRFLAALVER
                     DGRTAGTKAMIELLQWGRRHGQERLRAAVEHALALGCWDVAAVRHLLSADDLAHHQPP
                     PLAVGDLERFDRPFPTMDAYDQLLAAGDPGRIGVQLGAPEVVR"
BASE COUNT          301 a          768 c          768 g          338 t
ORIGIN      
        1 aatgtgtttg tcaatccggt gtggcccact tcgctgctcg ggagtggccc acccccaagg
       61 ggtatcctga gctactcggc ggcggcctcc tttcctttgc tcccgtggtt tcgctgcttg
      121 agcgtgcgcc ggaagcggaa cgaatcggta cccgtctcga tgatgtgcgc ccggtcggtg
      181 atgcgatcca agagcgcctt gcagaggcgg gcgttgggga tcacctgggt ccactcggag
      241 aagggcaggt tggtcgtcag gaggacggcc gccttctcgg cgcgctcggc gatgatctgg
      301 aagaggaact cggcgccgac ctcggccagg gggacgtagc cgacctcgtc gatggcgatc
      361 acgtcgtagc gcgcccagcg gcccaggacg cgccccagct ggagttgctg cttggcctcg
      421 accagctcgg tgacgagggc ggcggccgtg gtgaagcgca cgcggcgctt ctgccggcag
      481 gcggcgacgc agaggccggt caacaggtgc gtcttccctg tcccgcagtc gcccaggaag
      541 accaccggct ccgcgcgctc gatgtagccg ccttccgcca gctggtggag ctgggcggcg
      601 gagacggccg gcgtctgggc gaaatcgaac tcgtccagcg tcttgatgcg gggaaggtgc
      661 gcctcttgca gccggcgggc gaccgcccgc tgctcgcgat cctccagttc ggcggcgaac
      721 agcgccgcca ggtagtcgag atgcggatgc tgctcgcgct gggcctgctc ggcgagcggg
      781 aggcactggg cgaagaccgt tggcaagccg agctgcttgc actgctgctg gacgatgcgg
      841 ccctgcaagc cggtgggctg atcgctcatc gcaccacctc cggggcgccc agttggacgc
      901 cgatccggcc ggggtctccc gccgcgagca gttggtcgta agcgtccatg gtcgggaagg
      961 ggcggtcgaa gcgctccaga tcgccgaccg ccaggggcgg cggttggtgg tgcgcgagat
     1021 catcggcgct cagcagatgc cgcacggcgg caacatccca gcagcccagc gcgagcgcgt
     1081 gttccaccgc cgcccgcagc cgctcctggc cgtgccggcg gccccactgc agcagctcga
     1141 tcatcgcttt ggtcccggcc gtgcgcccgt cgcgctcgac cagggcggcg aggaagcggt
     1201 cgtagctctc gggccagcgg cccagccgcc gccattcggc caagggtttg ctgccggcga
     1261 gcgcccccgg cttgtgctcc agcacatcga ggtaatgttc gaggtccagg atttcccggc
     1321 cgcggccgta gcagcgttcg tgagcagcca cgcgtttgcc accgtgccag agttcgaccg
     1381 tcgccggcag caccttgatc tggacctgcg tcccgggtgg caaggggacc gagtaggcgt
     1441 tggtgcgcac ccgcacccgc cccagcgtgt ccaccgtggc gaagctggtc tcggccaggt
     1501 cgaagccttc gtcggcgagg ggcagcaggt gctcccgctc aagcaagagc gccaccccca
     1561 ccggccgctc ccgccccgcc agcacgcgcg cctcgtcggc gcgacagccg gcgagcaggt
     1621 gggcgttgag cgcggcgagg tcggcgacgt gggggatcgg gacccagtgg ttgcgccggt
     1681 acgtcccggc ctcgttctcg atcccgccct tctcgtgacc ctcgccgggc gtgcagaact
     1741 cggccgcgta gcgccagtgg gagcgaaagc cgacgaagcg cgtcgtctcc tcccggcgat
     1801 ggccgcgcag gaccttctgc accgccgccc ccaggttgtc gtagcgcagc cgccggaaca
     1861 cgccgccaaa gtaggcgaag gccagctcgt gcgcctccag gaaggcttgc tgcgtcgccc
     1921 gcgggtaggc ccggtggaag gcggcgccgc tcgccatact gcgcatgctg aatacctgcc
     1981 aggtctgcgg cgtgccgtcg accaccgcga ccgcctcgta ccagtccacc tgcgcctcct
     2041 cgccccaggc gtattcctgg ggcacgcagg tctcccgcgc caagagcccc aacgtgcgct
     2101 tgcgctcccg cacgtattgg cgcactgtgg actccccgat cacgagtcct ggccgctcct
     2161 cacggaggcg cgtcc
//
LOCUS       DASUBI010000422         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_656626, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000422 DASUBI010000000
VERSION     DASUBI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656626"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="VFU72_08100"
     CDS             complement(<1..2110)
                     /locus_tag="VFU72_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5423484.1"
                     /translation="MEIVSGVAGVPPRTAAGADGLIGLSAAQVEQRRARGELNVVPPP
                     STRSYRRILREHAFTPINTLLYAIAIVLIVLGLYGDALVTGGLVVANVVVGMTQETRA
                     KRKLDRIALLARPRATVIRDGREQVIDPAEIVRDDLLVLKPGGQVLVDGTLIRANALT
                     VDESLLTGEADPVPKTAGSRVVSGSFAVAGGGVYQAEQVGEHSLAQRMTESARAFRVV
                     QTPLQREVGLVLRAIAFLAVALTVQWAVAYYRAHHDLPLRESVQAAAVLVSLVPQGLS
                     LMTTVVYAIAAVRMSGKGALIQRMNAIESCSHVDTLCLDKTGTLTSNKLALREVRSLA
                     PDLTAAQVRARLEVFAASVSDGNRTIDAILADGPGVRRPIRDEVPFTSARKWSGLYLE
                     DAGAGGIYILGAPEVLARAMAPSADFAELEQEWQEAGLRVLLFAYRPDATPLHDNRGE
                     PRLPGDLQALALLSFADELRDEAQATLDRFTEAGVSPKIISGDHPETVAALARQAGIH
                     STKTVSGLDLDNWDDAQLQVVVQDVNVFGRVTPSQKERIVRLLRRDGHYVAMIGDGVN
                     DVPALKQADLAVAMASGSEVSRAVADVVLLDDSFAALPRAFQEGQRILRGMEDIVCLF
                     LARTLSLAFVILVVALFAVDFPVTPKHNSLLALLTVGIPTLAMAAWAQPGLTPRHIIV
                     STTRFVLPAAATIGFVCLIVF"
     gene            2242..>2886
                     /locus_tag="VFU72_08105"
     CDS             2242..>2886
                     /locus_tag="VFU72_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5423485.1"
                     /translation="MSQRRKRVLLLVTPSSYRSGAFAAAAERLGIEVVQGVDVPPTLA
                     AEWNVPLGLDFSDPAGAAAAIERYAREEPVDAIISVDDSATILASLASRLLGLAHNDP
                     EAALAARDKLPMRRLLASGGVPVPAFAAYPAISDPQRLASELDYPVVVKPTQLSGSRG
                     VIRADDAGDFVRAFERTRQVIEYAGQPAESAEILVEEFLPGFEVALEGLLDAGQL"
BASE COUNT          466 a         1026 c          967 g          427 t
ORIGIN      
        1 agaagacgat caagcagaca aagccgatgg tggccgccgc cgggagcacg aaacgcgtgg
       61 tcgacacgat gatgtgccgc ggcgtcaagc ctggctgcgc ccaggccgcc atggccagcg
      121 tcgggatgcc gacggtcaac agcgccagaa gcgagttgtg cttgggcgtc accgggaagt
      181 cgaccgcgaa gagcgcgacc accaggatca cgaacgccag cgacagcgtg cgcgccagaa
      241 agaggcacac gatgtcctcc atgccgcgca ggatacgctg accctcttgg aaggcgcgcg
      301 gcagagcggc gaaggagtca tcgagcagca ccacgtcggc caccgcgcgg ctgacctcgc
      361 tgccgctggc catggcgacc gccagatcgg cctgcttcaa cgccggtaca tcgttgacac
      421 cgtcgccgat catcgctacg tagtgaccat cgcggcgcag caggcgcacg atgcgctcct
      481 tctggctggg agtgacgcga ccgaacacat tcacgtcttg caccaccacc tggagttgcg
      541 cgtcgtccca gttatcgaga tcgagcccag acaccgtctt ggtcgaatgg atgcccgcct
      601 ggcgcgccag cgcggcgacc gtctctgggt gatcgccgga gatgatcttg ggactgacgc
      661 cggcctcggt gaagcgatcg agcgtggcct gcgcctcgtc gcgcagctcg tcggcgaagg
      721 agagcagcgc cagcgcttgc aggtcgcctg gcaggcgcgg ctcgcctcgg ttgtcgtgga
      781 gcggcgtcgc gtccgggcga taggcgaaga ggagcacgcg cagcccagcc tcctgccact
      841 cctgttctag ctcggcaaag tccgcgctcg gggccatcgc ccgggcgagc acctccggcg
      901 ctccgaggat gtagatgcca ccggctccgg cgtcttccag gtacagaccg ctccacttgc
      961 gcgccgaggt gaaggggact tcatcgcgga tgggccgccg cacccccggc ccatcggcga
     1021 ggatcgcgtc gatcgtgcgg ttgccgtcgg agacactggc cgcgaagacc tccagccgcg
     1081 cgcgcacctg tgccgccgtg aggtcggggg ccagcgagcg aacctcgcgc agcgcgagct
     1141 tgttgctcgt cagcgtgccg gtcttgtcca ggcagagagt atccacgtgg ctgcacgact
     1201 cgatggcgtt catgcgctgg atcaaggcac ccttgccgga catgcgcacc gccgcgatcg
     1261 cgtagacgac ggtcgtcatc agcgacagtc cctgaggcac gagagaaacc agcaccgccg
     1321 cggcctgcac gctctcgcgc agcggcaggt cgtggtgggc gcggtagtag gcgacggccc
     1381 attgcaccgt gagcgccacc gccagaaagg cgatggcccg cagcaccaag ccgacctccc
     1441 gctgcagcgg cgtctgcacc acgcgaaagg cccgcgcgct ctcggtcatg cgctgcgcca
     1501 ggctgtgttc gccgacctgc tcggcctggt agacgccgcc ccccgcaacc gcgaaactgc
     1561 ccgagacgac acgcgaaccg gccgtcttgg gcaccgggtc ggcttcgccg gtcaagagcg
     1621 actcatcgac cgtaagcgcg ttcgcccgta tcaacgtgcc gtcgaccagc acctgcccgc
     1681 ctggcttgag caccagcaga tcgtcgcgca cgatctcggc ggggtcgatc acctgctcgc
     1741 gcccgtcgcg gatcaccgtg gcacgcggcc gcgccagcag cgcgatgcga tcgagcttgc
     1801 gcttggcgcg tgtctcctgc gtcatgccga ccacgacgtt ggcgactacc agcccgccgg
     1861 tcaccagcgc gtcgccgtag aggccgagca cgatcagcac gatcgcgatc gcgtagagga
     1921 gggtgttgat cggagtgaag gcgtgctcgc gcaggatgcg ccggtagctg cgtgtgctcg
     1981 gcgggggaac gacgttcagc tcgccccgcg cccgccgctg ctcgacctgg gccgcgctca
     2041 gtccaatcaa gccatcggcg ccggccgcgg tacgcggcgg tacgcccgcc acgcccgaaa
     2101 cgatctccac gcctgccccc tcgaagatgt cgtccacgac ggagatgcgg tccccgccgg
     2161 caatcttact gtgctggcct accgctgtga gcggtatagt agattcgatc gcgagacccg
     2221 ccctcgcctg gagatgatgc gatgagccag cgacgcaagc gagttctcct cctggtgacc
     2281 ccctctagct accgctccgg cgcctttgcc gcggcagccg agcggctcgg aatcgaggtc
     2341 gtccagggtg tcgacgtgcc gcccacgctg gccgccgaat ggaacgtccc gctcgggctc
     2401 gacttctccg atccggcggg ggcagccgcg gccatcgagc gttacgcccg cgaggagccg
     2461 gtggacgcga tcatctcggt cgacgacagc gccaccatcc tggcctcgct cgccagcagg
     2521 ctgcttggcc tggcgcacaa cgaccccgaa gccgccctgg ccgcgcgcga caagctgccg
     2581 atgcgccgcc tgctggcgag cggcggcgtg ccggtcccgg ccttcgccgc ctaccccgct
     2641 atcagtgatc cccagcggct cgcctccgag cttgactacc ccgtggttgt caagcccacc
     2701 cagctctccg gcagccgggg cgtgattcgc gcggacgatg ccggagactt cgtgcgcgcc
     2761 ttcgagcgca cgcgccaggt catcgagtat gccggccagc cggcggagag cgcggagatc
     2821 ctggtcgagg agttcctgcc cggcttcgag gtagcgctcg aaggattgct cgacgccggc
     2881 caattg
//
LOCUS       DASUBI010000423         8184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1100081, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000423 DASUBI010000000
VERSION     DASUBI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100081"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFU72_08110"
     CDS             complement(<1..930)
                     /locus_tag="VFU72_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5423486.1"
                     /translation="MAQDFPFYCAGEWRTSRQTHQVTNPYNGETVGVTSFASEQDYED
                     AVSAARKAFEETRKLQSYERAEILQRMADALAARQEEFAQIMARESGKPIRDSRTEVA
                     RGVFTLQTAVEESKRITGEVIPLDLMASSAGRFGLTRRFPLGPIAGISPFNYPLNLAL
                     HKLAPAIASGNTIVLKPPTHDPLSMLTFAQIVDEAKLPKGAVSIMPMTRQTGDRMVTD
                     ERFKLLSFTGSPNVGWDMKSRAGKKKVVLELGGNAGVLVDQDADLDLAVARSRVGAFS
                     YAGQVCISVQRIYIHRARFDEFTEKFLQAVGQIK"
     gene            complement(1080..1970)
                     /locus_tag="VFU72_08115"
     CDS             complement(1080..1970)
                     /locus_tag="VFU72_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423487.1"
                     /translation="MRSVRFIGAALFGAAMLALVLVLVLTLSGLGNAATSAAPGTVTT
                     KTATASGTPAPPPQPPKPCCLTPAQQAISDAEWAAAEHDLGLDSDQFIAALKIGTSVD
                     QLATEHQTTTEKVRADMVAAGRDAVAAALRGGAIDQAEADTLNNSLVTAIADKVTHAN
                     VEPSNPATPTSGDKDLMHQKSAGANTSATFIMVNEAELHALATKLGVSDDQIKADLAG
                     CLSVAALNGHSADQAQIRDVMVAAGQSAVDAAVQRGQVTPAQADQLRQSVVTAFADKV
                     SSALTNAGSSPSTCSATPAA"
     gene            2206..2907
                     /locus_tag="VFU72_08120"
     CDS             2206..2907
                     /locus_tag="VFU72_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423488.1"
                     /translation="MRILVVEDNRRLSVGLKRCLEAEGYAVDCAYDGTEGLDYAEAAP
                     YDAIVLDLMLPGRDGVEVCRVLRLRGYAAPILMLTARDAVEDRVRGLDSGADDYLVKP
                     FALEELHARLRALLRRQLPQRGGSIVLGDLEVDPATHGVARAGRPVVLTAREFALLEL
                     FVRHPNQIITREMAESHIWSYDYEGTSNVVDVYVRRLRRKLDDPFAHRLIVTVRGVGY
                     RLCAPVQGTGTDAER"
     gene            2894..4579
                     /locus_tag="VFU72_08125"
     CDS             2894..4579
                     /locus_tag="VFU72_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5423489.1"
                     /translation="MLNGSWRARVPSPRRFTNIRWRLTLWYLVILAVVLAVFGTLIYQ
                     SQARSLRAAQDESLRQQARQLAVGYNPSNGAFDAATSGLDQQKRALTGSPVADGAALG
                     QFPPGTFVLGNIVLLISSDTKITGQMAGVGPVDAARIFAPADLQRLLQLGAPAGPPRA
                     SDASFSALVAAGNAAPAKREMYRFYSTPILVKNVVFGTLVVGRPDSAAAQLHRLLLTL
                     LLAAPLTLLVASAGGYWLAARAMRPVRRIAATVREISGSDLSRRVELRTGDELGELAA
                     TFDQMLDRLEGAFRRQRQFTSDASHELRTPLTIVRLELARALADPALSPETARALVNV
                     GAENDYMAGLVDDLLLLARADSGRVVLRREMLDLSDLALGVVERLAPLARERGLRLVV
                     GALPELPITGDRVYLTQMLANIVENGIKYTAGWGSRVTIEGGASSHADPHAWVRVGDD
                     GPGIAPEHLPHLFERFYRVDAARVRDDSGTAPRVAATSGTGLGLAIVDWIVHAHDGEV
                     RITSHSGEGTTVEVQLPLEPGADQTGDEHCRPARGAMTSGASLAARAPGDRCL"
     gene            complement(4607..5527)
                     /locus_tag="VFU72_08130"
     CDS             complement(4607..5527)
                     /locus_tag="VFU72_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423490.1"
                     /translation="MAVRASQHGGQDVERDVLVGGIAQRHVSRGRQFWRSYARSKGAV
                     LSAAILILLVLMAVAAPWIEPHDPLGVSAGEFLNGPTLSHPFGTDQYGRDVLSRVISG
                     SRISLTIGGLAVIFSVLFGTALGLVAGYSENVLDSVIMRLIDMLLAFPGILLALAIVA
                     GLGASTINVIIAIGVGGIPVYARVARGSVLSAKREAYVDAGRVIGCSAPRLMVRHILP
                     NIFAPVLVLATIGIAFSILIGAALSYLGLGIQPPRAEWGLMISDGQNYLNDAWWISTF
                     PGIALAITVLCVNLVGDGLRDAFDPRTRRR"
     gene            complement(5550..6473)
                     /gene="nikB"
                     /locus_tag="VFU72_08135"
     CDS             complement(5550..6473)
                     /gene="nikB"
                     /locus_tag="VFU72_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HEU5423491.1"
                     /translation="MATYIVRRLLVGIPVIWGVATIVFFLLRILPGDPAQLMLQGTGG
                     NADQIARLRHNLGLDQPLLVQYEHYITNALQGDLGTSLRTNIPVTQEIRQQFPATLEL
                     TAAGMLIAIVLGMTLGIVSAIWQHSALDSLATTVAMFGICAPSFWLGLLLIFFFSFRL
                     GWFPATGTWGLDHLVLPAITLGLGAAAVIARLVRSSMIEALSQDFIMVARAKGLSQAA
                     VTLRHALKGALIPAVTLIGLQFGSLLGGAVIIETVFSRQGVGHLAVQSILAKDYTTVQ
                     GIVLFIAIAYVVVNLVVDLLYGFLDPRIRYA"
     gene            complement(6504..>8184)
                     /locus_tag="VFU72_08140"
     CDS             complement(6504..>8184)
                     /locus_tag="VFU72_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423492.1"
                     /translation="SRRALLRQGSVAAGAVGLTGLLAACGSSGSTPKSNGASGSGNAT
                     AASGTANATEAAGKPVEGGTFTYALNQEPESLDPEVTTYAVTNKININVFDPLVWQAP
                     DLSFVPGLSEKWDVTPDATSFTFHLRTDVKFHDGTQLNADAVKFSWDRIMNPETKSKT
                     ALNQMGPFKQATVVDPSTIKADFSESFAPFLDAASQSYCAPVSPAAVQKFGDQFAFNL
                     VGTGPYKLKEWVKGDHVTLVKNPDYNWAPSIFKHKGPGYLDQIIFKVVIENATRTGLL
                     QSGQVDGIDSVPAKDVASFKADPSKFQIMLAEAPGIPTLLVMNTTKFPTDDVAVRKAL
                     EYGTDKEAVIKVLDFGLYTAAYGPLTSVTLGYDDSLKTMYPYDPKKATQTLEDAGWKK
                     GSDGFYAKDGKQLVLDYYASNVDMTPPLLQAQWKSIGINTNIKAMDYNAAIPLVTKGE
                     ANVASIGWIQADPDVCRIILYSKNIGTGYGWSFFKNDELDQTIIKAASSVDPAVRKPL
                     YAKVQQITMENALVLPITNLFAIYGLQSYVKNFRVDTRGWYPWLYDVYLQK"
BASE COUNT         1344 a         2602 c         2828 g         1410 t
ORIGIN      
        1 cttgatctgg ccgaccgcct gaaggaattt ctcggtgaat tcatcgaagc gggcgcgatg
       61 gatgtagatg cgctgcaccg agatgcagac ctggccggcg tagctgaagg cgccgacccg
      121 gctgcgcgcc accgccaggt cgaggtcggc gtcctggtcg accagcaccc cggcgttgcc
      181 gcccagctcc agcaccacct tcttcttgcc ggcgcgggac ttcatgtccc agccgacatt
      241 cggtgagccg gtgaaggaga ggagcttgaa gcgctcgtcg gtcaccatgc ggtcgcccgt
      301 ctggcgcgtc atcggcatga tgctgaccgc acctttgggc aatttggcct cgtcgacgat
      361 ctgggcgaag gtcagcatcg agagcgggtc gtgcgtcggc ggtttgagca cgatcgtgtt
      421 gccgctggcg atggccggtg ccagcttgtg cagcgcgagg ttcagcgggt agttgaaggg
      481 cgagatgccg gcgatggggc cgagcgggaa gcggcgggtc aggccgaagc gcccggccga
      541 ggaggccatc agatcgagcg ggatgacctc gccggtgatt cgcttcgact cctcgaccgc
      601 cgtctgcagg gtgaagacgc cgcgggcgac ctcggtgcgc gagtcgcgga tcggcttgcc
      661 cgactcacgc gccatgatct gggcgaactc ctcctgccgc gcggccagcg cgtcggccat
      721 gcgctggagg atctcggcac gctcgtagct ctgcagtttg cgcgtctcct cgaacgcctt
      781 ccgcgcggcg ctgaccgcgt cttcgtagtc ctgctcgctg gcgaaggagg tgacacccac
      841 cgtctcgccg ttgtaggggt tggtgacctg gtgggtctgc ctggacgtgc gccactcacc
      901 ggcgcagtag aaggggaagt cttgcgccat cgaaagcctc ctggttccgc gccccatcca
      961 gcggggcgtg gctcgatctt agtccggcct ccgcgggggc gtcaacgcgc aggcgcggcc
     1021 ggcgcctcac tggaggcagt gaagcgccgg ccgcgaggga aggggagatt ggcggccggt
     1081 taggcagccg gggtagccga acaggtggaa ggcgacgagc cggcgttcgt gagcgccgag
     1141 ctgactttgt ccgcgaaggc ggtcacgacg ctctggcgca gctgatcggc ctgagctgga
     1201 gtcacctggc cgcgctggac ggccgcgtcg accgccgact gcccggccgc gaccatcacg
     1261 tcgcggattt gggcttggtc ggccgaatgc ccgttgagcg cggcgaccga gaggcagccg
     1321 gccaggtccg ctttgatctg atcgtccgag acgccgagct tcgtcgccag ggcgtgcaac
     1381 tccgcctcgt tgaccatgat gaaagttgcg gacgtgttcg cgcccgcaga cttctggtgc
     1441 atgagatctt tgtcgccgga cgtcggggtg gccgggttcg acggctcgac attggcgtgc
     1501 gtcaccttat cggcgatcgc ggtgacgagg ctgttgttga gcgtatccgc ctcagcctgg
     1561 tcgatggcgc cgccgcgcag cgccgcggcc accgcatccc gtccggccgc gaccatgtcg
     1621 gcgcggactt tctccgtcgt ggtctggtgc tccgtcgcga gctgatcaac ggacgtcccg
     1681 atcttcaagg cagcgataaa ttgatcagaa tccaggccga gatcgtgttc ggcggcggcc
     1741 cactcggcgt cggagatcgc ctgctgggcg ggtgtaaggc agcacggctt aggcggctgc
     1801 ggcggtggcg ccggtgtgcc gctggccgtg gccgtcttgg ttgtgaccgt gcccggagcc
     1861 gccgaagtag cggcgtttcc cagtccgctg agagtcagca ccagcaccag caccagcgcc
     1921 agcatcgccg ctccgaagag cgccgcaccg ataaagcgaa ccgagcgcat ggcataccac
     1981 ctttcaactc ctgtcgtatc cgccacgccg gtgcttgact cggtcgaagc accggtccga
     2041 ctcatcctgc gcgcgtgaga tgacacgaga atgacacctg gacggctgcc accagttgct
     2101 cctcgggcgg atctgtcatg gagttgtcat cgaagcgtgg tgtaatgcgg caagaagccg
     2161 cccgcgcggt agtgcgccga ggcttgccga ggaggacacc ggctgatgcg catcctggtc
     2221 gtggaggaca atcggcgtct aagcgtcggg ctgaagcgct gcctggaggc cgagggctac
     2281 gcggtcgact gcgcctacga cggcactgaa gggctcgatt acgccgaagc ggcgccctac
     2341 gacgcgatcg tgctggatct gatgctcccg gggcgcgacg gtgtcgaagt ctgtcgtgtg
     2401 ctgcgcctgc gcggctatgc tgcgccgatc ctcatgctga cggcgcgcga tgcggtcgag
     2461 gaccgcgtgc gcgggctcga cagcggcgcc gacgactact tggtcaagcc gttcgcgctg
     2521 gaggagctgc acgcccggct gcgcgcgctg ctgcgtaggc agcttcccca acgcggcggc
     2581 tcgatcgtgc tcggcgattt ggaggtcgac ccggccacgc acggcgtcgc gcgcgccggc
     2641 cggccggtcg tgctgacggc gcgcgaattc gctttgctgg agctattcgt gcgccatccc
     2701 aaccagatca tcacccgcga gatggccgag tcgcatatct ggagctacga ctacgaaggc
     2761 acctcgaatg tggtcgatgt ctacgtgcgc cgcctgcgcc gcaagctcga cgatcccttc
     2821 gcccatcggt tgatcgtgac ggtgcgtggc gtcggctacc gcctgtgtgc accagtgcag
     2881 ggtacgggaa ctgatgctga acggtagctg gcgcgcgcgc gttccgtcgc cgcgtcgctt
     2941 caccaacatt cgctggcgcc tcacgctctg gtatctcgtg atcctggcgg tggtgctggc
     3001 cgtcttcggt acgctcatct accagtcgca ggcgcggagc ctccgcgccg cgcaggacga
     3061 gtcgctccgg cagcaggccc ggcagctcgc cgtgggctac aacccttcca acggtgcgtt
     3121 cgacgcggcg acatcgggtt tggaccagca gaagcgcgct ctgactggct cccccgttgc
     3181 cgatggggca gcgctgggac agttcccgcc cggtacattc gtgctcggca acatcgtgct
     3241 gctgatcagc agcgacacca agatcacggg tcagatggcg ggcgttggtc cggtcgacgc
     3301 ggcgcgcatc ttcgcgcctg ccgatctcca gcggctgctg cagctcggcg caccggctgg
     3361 cccgccgcgc gcgtccgacg cctctttcag cgccttggtc gcggccggca acgcggcgcc
     3421 cgctaagcgc gagatgtatc gcttctacag cacgccgatt ctggtcaaga atgtcgtctt
     3481 cggcacgctg gtggtcggcc gtcccgatag cgcggcggcc cagctccatc gcttgctgct
     3541 cacgctgctg ctggccgcgc cgctgacgct gctggtcgcc agcgccggag gctactggct
     3601 ggccgcgcga gccatgcggc cggtgcgccg catcgccgcc accgtgcgcg agatcagcgg
     3661 cagcgatctg tcgcgccggg tcgagctgcg gaccggcgac gagctgggcg aactggccgc
     3721 gaccttcgat cagatgctcg atcggctgga aggcgccttc cgacgccagc gtcagttcac
     3781 cagcgacgcc agccacgagc tgcgtacgcc cctgacgatc gtgcggctcg agctggcgcg
     3841 cgcgttggcc gaccccgcgc tctcgccgga gacagcccgc gctctggtca acgtgggcgc
     3901 cgagaacgac tacatggcgg ggctggtgga cgatctgctg ctgctggcgc gagccgacag
     3961 cggacgagtg gtgcttcggc gggagatgct tgacctcagc gatctggccc tgggggtggt
     4021 cgagcggctg gcgccgctcg cgcgcgagcg cggtctccgg cttgtggtgg gtgcgctgcc
     4081 ggagttgccc atcaccggag atcgtgtcta tctgacccag atgctggcca acatcgtcga
     4141 gaacggcatc aagtacaccg ccgggtgggg aagccgggtc acgatcgagg gaggcgcgtc
     4201 atctcacgcg gacccgcacg cctgggttcg cgtgggcgac gatggcccgg gcatcgcgcc
     4261 ggagcacctg ccgcacctct tcgagcgctt ctaccgggtc gacgcggcac gcgtccgcga
     4321 cgactcgggc accgcgcctc gtgtcgctgc cacgtccggc accggcctag gcctggcgat
     4381 cgtcgattgg atcgtccacg cgcacgacgg cgaggtgcgg atcacgagcc actccggtga
     4441 aggcacgacg gtcgaggtcc agttgccgct ggagcccgga gcggaccaga ccggcgacga
     4501 acattgccgg ccagcgcgcg gtgccatgac cagcggcgca tccttggcgg cgcgtgcgcc
     4561 aggggaccgc tgcctctgaa caggcgtgat cgtcagccga tcctcgctag cggcggcggg
     4621 tgcggggatc gaacgcgtcg cgcaggccgt cgccgacgag gttgacgcag aggacggtga
     4681 tggccagcgc gatgccgggg aaggtcgaga tccaccaggc gtcgttgagg tagttttggc
     4741 cgtcggagat catcaggccc cactcggcgc gcggcggttg aatgcccagt ccgagatagg
     4801 agagcgccgc accgatcaag atggaaaagg cgatgccgat cgtggccagc accaggaccg
     4861 gcgcgaagat gttgggcaag atgtgccgca ccatcaggcg cggcgccgag cagccgatca
     4921 cgcggccggc atcgacatac gcctcgcgct tggccgagag cacggacccg cgagccaccc
     4981 gagcgtagac cggaatgccg ccgaccccga tggcgatgat cacgttgatg gttgaggcgc
     5041 ccagcccggc gacgatcgcc agtgccagca gaatgccggg aaacgccagg agcatgtcga
     5101 tcagccgcat gatcacgctg tcgagcacgt tctcgctgta gccggcgacc agccccagcg
     5161 cggtgccgaa gagcacgctg aagatcacgg ccagcccgcc gatggtcagc gagatacggg
     5221 agccggagat gacgcgcgag agcacgtcgc ggccgtactg gtcggtgccg aacgggtggg
     5281 acagggtcgg gccgttgagg aactcccccg cggagacccc gagcgggtcg tgtggttcga
     5341 tccagggcgc ggcgacggcc atgagcacga gtagaatgag gatcgcggcg ctgagcacgg
     5401 cccctttgct gcgcgcgtag ctgcgccaga attgccgccc gcgcgagacg tggcgctgtg
     5461 cgatgccgcc gacgagcacg tcgcgctcga catcctgacc accatgctgg cttgctcgta
     5521 ctgccatcac gcgcccatct tacggccgtc tacgcatagc gaatccgcgg atcgagaaag
     5581 ccgtagagca gatcgaccac gagattcact acgacgtagg cgatggcgat gaagaggacg
     5641 atgccctgaa cggtggtgta gtccttggcc aggatcgact ggacggccag atggcccacg
     5701 ccctggcggg agaagaccgt ctcgatgatc accgcgccgc ccagcaggga gccaaactgc
     5761 aggccgatca gcgtgaccgc ggggatgagc gcacccttca gcgcatggcg cagtgtgacc
     5821 gccgcctggg agaggccctt cgcgcgcgcg accatgatga aatcctggct cagggcttcg
     5881 atcatcgagg agcgcaccag gcgagcgatg acggcagccg cacccagccc cagcgttatg
     5941 gccggcagca cgagatgatc gagcccccag gtgccggtgg ctggaaacca gcccagtcgg
     6001 aacgagaaga agaagatcag cagcaggccc agccagaagc tgggggcaca gataccgaac
     6061 atcgcgacgg tggtggccag gctgtcgagc gcgctatgct gccagatcgc ggagacgatg
     6121 ccgagcgtca tacccagcac gatcgcgatc aacatcccgg ccgcggtcag ttcgagggtc
     6181 gcggggaatt gctgcctgat ctcctgcgtc accgggatgt tcgtgcgcag ggaggtgccg
     6241 agatcacctt gaagcgcgtt ggtgatgtaa tgctcgtatt ggaccagcag cggctggtcc
     6301 agcccaagat tgtgccgcag gcgcgcgatc tggtcggcat tcccgccggt cccttgcagc
     6361 atcaactgtg cggggtcacc gggcaggatg cgcaggagaa agaagacgat ggttgccacg
     6421 ccccaaatga cggggatgcc gaccaataaa cgtcgaacaa tatacgtggc cattggctca
     6481 accagccctc aagcgccgcg cgcctacttc tgcaggtaca cgtcgtacag ccacggatac
     6541 caaccgcgtg tatcgacgcg gaagttcttc acgtagctct gtaggccgta gatcgcgaag
     6601 agattggtga tcggtagcac caaggcgttc tccatcgtga tctgctgaac tttggcgtac
     6661 agcggcttgc gcacggccgg atcaaccgac gaagcggcct tgatgatcgt ctggtcgagc
     6721 tcgtcgttct tgaagaacga ccagccgtag ccggtgccga tattcttgga gtagagaatg
     6781 atccggcata cgtcggggtc cgcctggatc cagccaatcg aggccacgtt ggcttcgcct
     6841 ttggtgacca acgggatggc ggcgttgtag tccatggctt tgatgttggt gttgatgccg
     6901 atggacttcc actgagcttg cagcagcggc ggggtcatgt cgacgttgct ggcgtagtag
     6961 tcgaggacga gctgcttgcc gtccttggca tagaacccgt cggagccctt tttccagccg
     7021 gcatcctcga gcgtctgcgt cgccttcttg ggatcgtagg ggtacatcgt cttcaggcta
     7081 tcatcatagc ccagggtgac cgacgtcagc gggccgtagg ccgccgtgta gagcccgaaa
     7141 tcgagcactt tgatgacggc ttccttgtcg gtgccgtact ccagcgcctt gcggaccgcg
     7201 acgtcgtcgg tcgggaactt ggtggtgttc atcaccagga gggtggggat accgggcgct
     7261 tcggcgagca tgatctgaaa cttgctggga tcggccttga agctggccac atccttggcg
     7321 ggcacggagt cgataccatc gacctgcccg ctctggagca ggccggtgcg cgtggcattc
     7381 tcgatgacga ccttgaaaat gatctggtcg agatagcctg ggcccttgtg cttgaagatc
     7441 gacggcgccc agttgtagtc cggattcttg acgagggtaa cgtgatcccc cttgacccat
     7501 tccttcaact tgtagggccc ggtgccgacg aggttgaacg cgaactgatc gccgaacttc
     7561 tggaccgcgg ccggcgacac cggtgcgcaa tacgactggc tggcggcatc gaggaacggc
     7621 gcgaacgact cggagaaatc cgccttgatc gtcgacggat cgacgacggt tgcctgcttg
     7681 aagggcccca tctggttcag ggcggtcttc gacttcgtct ccgggttcat gatgcggtcc
     7741 cacgagaact tgaccgcatc cgcgttcagt tgcgtgccgt cgtggaactt gacgtcggtg
     7801 cgaaggtgga aggtgaatga cgtggcatcc ggcgtgacgt cccatttctc ggacagaccg
     7861 ggaacgaatg acaggtcggg cgcctgccag acgagtggat cgaagacgtt gatgttgatc
     7921 ttgttggtga ctgcgtaggt cgtcacctcc ggatcaagcg actccggctc ctgattgagc
     7981 gcataggtga acgtgcctcc ctcgaccggc ttcccggccg cctcggtcgc gttcgccgtg
     8041 ccactggccg cggtggcatt accgctcccg ctcgcgccgt tactcttggg agtgctgcct
     8101 gagctcccgc acgcggcaag aaggccggtc agcccgaccg ccccggcggc gacgctccct
     8161 tgccgcagca acgcgcgccg cgaa
//
LOCUS       DASUBI010000424         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_451576, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000424 DASUBI010000000
VERSION     DASUBI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451576"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFU72_08145"
     CDS             <1..531
                     /locus_tag="VFU72_08145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU5423493.1"
                     /translation="GHTQRVAELSVLLGQELGLSADQLRTLNQAAMLHDIGKIGIPDD
                     ILHKPGPLSVEEFAVIQEHPVRGEQIIRRVRSLQAELSGVRSHHERLDGSGYPDGLRG
                     AAIPLQARIIAVADVFDALTSHRPYRTAWPAERALAIIDQEAGSKLDPSCVAALHRVL
                     QVWAEAHPTHALRLAS"
     gene            802..2829
                     /locus_tag="VFU72_08150"
     CDS             802..2829
                     /locus_tag="VFU72_08150"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5423494.1"
                     /translation="MAQQVVPADVPAITLDEILAIKHPDPAVWSPDGQWLAWRWDDGG
                     VVQLWAAPAAGGAPLQVSRGGDTSVGGFSWSPEGELAYAHGEHVWLARPGQAPRRLTS
                     EAAPYGSPRWSPQGDALAYTRAGTLLLHDLASGDVRELPLPGQVIAGYADSVSLAWSP
                     GGSKLAAILRAGAQRDVAVVGRDGALVWRTDTPANEPAFAWVDDQRLHFTRMAVDHRV
                     RTHHLVDLASGEERELVHEESHRGLKAELPPVVAPDGQSVVYVLTPENWPHLFHYDLK
                     SGELRQLTTGDCDDTGHAGDSLSFAPDGRRLVYCSNRDTDLNQRRLWLVDVAGATSRL
                     LTPGPGTDSCAAWSPNGAAIAYLHCSPDQSADIWLVSPESRETRQVSHSMPATLGPER
                     LTAPRHLTYPSRDGLAVQADLFLPKGFDPARTYPAVVWVHGGMARQMRYGWHPMHTYA
                     LFYSYHQYLLHRGFVILSVDYRGSIGYGRDYEEGTYLQMLQGDLADVAAGADYLRTLP
                     YIDPEGIGVYGLSYGGYLTLGALTKHPEAFALGINLAGIWDWPQYEAWREAKYPGSNW
                     QGRARLGGPPGETNADAWYQASPKHFVAGLRAPLLNLMGTADERVDFAQLDVIIRDCV
                     AHQKDFAALYYPGESHTFTHRRTWRDAFPRIERAFQRYLTVPQEERPPAMI"
     gene            2899..3159
                     /locus_tag="VFU72_08155"
     CDS             2899..3159
                     /locus_tag="VFU72_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5423495.1"
                     /translation="MPERKLVRVQEKGQVTLPVEVRKRLGLIKGDLVAITETPEGVLI
                     TPQEVIATRTLDQIGAALRERGLSLEELIESGREARDDLLGE"
     gene            complement(3331..>3608)
                     /locus_tag="VFU72_08160"
     CDS             complement(3331..>3608)
                     /locus_tag="VFU72_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423496.1"
                     /translation="LAVLPMGVVEFDPLSGERRIDPEHPVLQSEATFAQTLAIVRALE
                     AKRVILTHIEEMDGLSYDDLQVVQANLQRQGLPIEFAYDTLVVDVDC"
BASE COUNT          562 a         1343 c         1152 g          551 t
ORIGIN      
        1 ggccacaccc agcgcgtggc cgagctctcg gtgctgctcg gccaggagct ggggctatca
       61 gccgaccagc tccgcaccct gaaccaggct gccatgctcc acgacatcgg caagatcggc
      121 atccccgacg acatcttgca caagcccggc cccctgagcg tggaggagtt cgccgtcatc
      181 caggagcacc ccgtgcgcgg cgagcagatc atccgccgcg tgcgctcgct ccaggccgag
      241 ctgagcggcg tgcgctcgca ccacgagcgc ctcgacggca gcggctaccc cgacggcctg
      301 cgcggcgcgg ccatcccgct ccaggcacgc atcatcgccg tggccgacgt cttcgacgcg
      361 ctcacctcgc accgccccta ccgcaccgcc tggccggccg agcgcgcgct ggcgatcatc
      421 gaccaggagg ccggcagcaa gctcgacccg agctgcgtgg ccgccctcca ccgcgtcctc
      481 caggtctggg ccgaggccca ccccacgcac gccctccgcc tcgccagcta gccgctgccc
      541 aacgaacacg ttgggtcccg ggcgaagacg gatccttcga caagctcagg atgacagggt
      601 tggttcgggg gcttcggggc gaggaccgtg ctcctcgtta gtcaccctat tagccctgtc
      661 atcccgaccg cagtggaggg atccgtcctg gcgcggtgct caagctcctt gatggtcacc
      721 gcttcgttcc cgcttggcct cccgcccctc ctgggcgata atccgcccac gagaccggac
      781 acaagcgaat tggggggcga ggtggcacag caggtcgtgc cggcggacgt gccggccatt
      841 acgctcgacg agattctggc gatcaagcac ccggacccgg ccgtctggtc gcccgatgga
      901 cagtggctcg cctggcgctg ggacgacggc ggcgtcgtcc agctctgggc cgccccagcc
      961 gccggcggcg cgccgctcca ggtctcgcgt gggggcgaca ccagcgtagg cggcttcagt
     1021 tggtcgccgg agggcgagct ggcctacgcc cacggcgagc acgtctggct ggcccggccc
     1081 ggccaggcgc cgcgccggtt gacgagtgag gccgcaccct acggctcgcc gcgctggtcg
     1141 ccccagggcg acgcgctggc ctacacccgt gccggcacat tgctgctgca cgacctcgcc
     1201 agcggtgacg tgcgcgagct gcccctgccc ggccaggtga tcgccggcta cgccgacagc
     1261 gtcagcctgg cctggtcgcc cggcggcagc aagctggccg ccatcctgcg cgctggcgcg
     1321 cagcgcgacg tggccgtcgt cggccgcgac ggcgcgctcg tctggcgcac cgacacgccg
     1381 gccaacgagc ccgccttcgc ctgggtcgac gaccagcggc tgcacttcac tcgtatggcc
     1441 gtcgatcacc gcgtgcgcac gcaccacctg gtcgatctgg cctcgggtga agaacgcgag
     1501 ctggtgcacg aggagagcca ccggggcctc aaggccgagc tgccgccggt cgtcgcgccc
     1561 gatggacagt cggtcgtcta cgtgctcacc cccgagaact ggccccatct cttccactac
     1621 gacctcaaga gcggcgagct gcgccagctc acgacaggtg actgcgacga caccggccac
     1681 gccggcgact cgctctcctt cgcgcccgac ggccgtcggc tcgtctactg cagcaaccgt
     1741 gacaccgatc tcaaccagcg ccgcctctgg ctggtcgacg tagccggcgc gacctcgcgt
     1801 ctgctgactc ccggccctgg caccgactcc tgcgccgcct ggtcgcccaa cggcgccgcc
     1861 atcgcctacc tgcactgctc gcccgaccag agcgccgaca tctggctggt gtcgcccgag
     1921 agccgggaga cacgtcaggt atcgcactcg atgccggcca cgctcggccc cgagcggctg
     1981 accgcgcccc ggcatctcac ctaccccagc cgcgatggcc tcgccgtcca ggccgatctc
     2041 ttcctgccca agggcttcga cccggcgcga acctatcccg cggtcgtctg ggtccacggc
     2101 ggcatggcgc gccagatgcg ctacggctgg cacccgatgc acacctacgc cctcttctat
     2161 tcatatcatc agtacctgct gcaccgcggc ttcgtcatcc tctcggtcga ctaccgcggc
     2221 agcatcggct acggccgcga ctacgaggag ggcacctacc tccagatgct gcagggcgac
     2281 ctggccgacg tggcggccgg cgccgactac ctgcgcaccc tcccgtatat cgaccccgag
     2341 gggatcggcg tctacggcct cagctacggc ggctacctca cgctgggcgc gctgaccaag
     2401 caccccgagg cctttgcgct gggaatcaac ctcgccggca tctgggactg gccgcagtac
     2461 gaggcctggc gcgaggcgaa gtacccgggg agtaactggc agggccgcgc tcgcctgggc
     2521 gggccgcccg gcgagaccaa cgccgacgcc tggtaccagg cctcacccaa gcatttcgtc
     2581 gcagggctgc gcgcgccgct gctgaacctg atgggcaccg cggacgagcg ggtcgacttt
     2641 gcccagctcg acgtcatcat ccgcgactgc gtggcgcacc agaaggactt cgcggcgctc
     2701 tactaccccg gcgagtccca caccttcacc caccgccgca cctggcgcga cgccttcccc
     2761 cgcatcgagc gcgccttcca gcgctacctc accgtgcccc aggaggagcg cccgccggcg
     2821 atgatttgaa cggcatctag gcggctcagg tgtcgggtat actttccgaa agtcatactt
     2881 tcggaagcga tgatcaccat gccagagcgc aaactggttc gcgtgcaaga gaaggggcaa
     2941 gtgaccttgc ccgtcgaggt caggaagcgg ctcggactca taaagggcga tctggtcgcc
     3001 atcaccgaga cgccagaggg tgtgttgatc accccgcagg aggttatcgc caccagaacg
     3061 ctcgatcaga tcggcgccgc actgcgagag cgcgggctct cgctggagga gttgatcgag
     3121 tccggacgcg aggcgcgcga cgacctgctg ggcgagtagg atggggtgga tgtcggcggt
     3181 cgtcgccggt ctaggctcat tgacgaacca attcggccca cttcgtgtac cttctcagcc
     3241 tgcttcagca ggcttcgttt actagcgcgg cggctttagc cccgcgcgat cccacggcat
     3301 cggtcgaatg aattcgtcaa actccattaa tcaacaatca acgtcaacca ccagcgtgtc
     3361 atacgcgaac tcgatgggca gtccctgacg ctggaggttg gcctgtacca cttgcaggtc
     3421 gtcgtagctc aggccgtcca tctcctcgat gtgggtgagg atcacgcgct tggcctccag
     3481 ggcgcgcacg atcgccagcg tctgcgcgaa ggtggcctcg gactggagaa ccgggtgctc
     3541 ggggtcgatg cggcgctccc cgctcagcgg gtcgaactcg accacgccca tcggcagcac
     3601 cgccaggt
//
LOCUS       DASUBI010000425         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_844121, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000425 DASUBI010000000
VERSION     DASUBI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844121"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="hrcA"
                     /locus_tag="VFU72_08165"
     CDS             <1..1039
                     /gene="hrcA"
                     /locus_tag="VFU72_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5423497.1"
                     /translation="ELSERQRAILRYIVQEYVRTGRAVGSKTLIERYELHVSSATVRN
                     EMGELEERAYLQHPHTSAGRVPTDRGYRYYVEYLLEETQLPPADQLMIRHQFRQVESQ
                     LDRSARLAASILSELSGNLALVTPPRSPIERLRHFELISLREQVALLIVVTQSGAVHQ
                     MVLPLEEPTEQTDLSALSQQINPEVRNGTAAQVLQRASELGGLPALVLQNVAEAIMRA
                     DQESQSALFSDGLERTLGQPEFTRSAVAQRLLELLRGGTILSALLPQLELEDDVQVFI
                     GSENKSEDLRSFGVVISTYGSGDEISGLLGVLGPTRMPYGRSISTVRYLAQVLSDLLG
                     SIYEESADPAE"
     gene            1069..1677
                     /locus_tag="VFU72_08170"
     CDS             1069..1677
                     /locus_tag="VFU72_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5423498.1"
                     /translation="MDEDRRESAPEQQAPEKTTGTDGMLDEQPVEPETMSTTVEQLAA
                     ERARAEEYLEQARRARAELINYRRRSEQEMEQARRNAGERIIARLLPVADDFARALGA
                     VPESEMQNPWIQGILLIERKLWSILEAEGVQPIEAVGQPFNPAVHEAVSMESGGGNEV
                     VAEYQRGYTLHDRVLRPAMVKVGQGKQNGQAAGSAQNGEPAS"
     gene            1709..>3568
                     /gene="dnaK"
                     /locus_tag="VFU72_08175"
     CDS             1709..>3568
                     /gene="dnaK"
                     /locus_tag="VFU72_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5423499.1"
                     /translation="MARTIGIDLGTTNSVMAVIEGGEPVVLPNAEGERLTPSVVAVTN
                     TGERLVGRFAKRQAVTNPSNTVFSVKRFMGRRFDDPNVQADLKLVPYEVRKAQNGGVE
                     IKMGDRWSSPPEISAMILQKLKTDAEAYLGDKVDRAVITVPAYFDDSQRNATKDAGRI
                     AGLDVLRIINEPTASALAYGLDKKNDEQIAVYDLGGGTYDISILDLSEGVFQVLATNG
                     DTHLGGDDFDQKIIDWLADEFKKQEGIDLRGDRMALQRLKEAAERAKIELSSTMQTEV
                     TLPFITADQSGPKHMNITLSRSKLEQLVGDLVDRTVPPMDSALKDANLSKGDVDEVIL
                     VGGQTRMPAVQRKVADFFGKEAHKGINPDEVVAVGAAIQAGVLQGEVKDVLLLDVTPL
                     TLSIETLGGVATPLIERNTTIPTRKSQIFTTATDNQTQVEIHVVQGERPMASENRSLG
                     RFILDRIPPAPRGVPKIEVTFDIDANGILNVTARDQATGREQKITITASSGLTEDEIK
                     RMVHEAESHAEDDRKRREAIELRNQAEAMAYQAERTLADYGDRIPSELKAELDNQIQA
                     VRTTLQQNPEDVESLRPAYEQLTQTLSKVGASMYEQPAAGGAPGEEQPGAQQAG"
BASE COUNT          688 a         1210 c         1196 g          474 t
ORIGIN      
        1 cgaactgagc gagcgacaac gcgcgatcct gcgctatatc gtccaggagt acgtgcgcac
       61 gggccgcgcg gtcggctcca agacgctgat cgagcgctac gagctgcacg tcagctccgc
      121 caccgtgcgc aacgagatgg gcgagctgga ggagcgcgcc tacctgcagc acccccacac
      181 ctccgccggc cgcgtgccga ccgaccgcgg ctaccgctac tacgtcgaat acctgctgga
      241 ggagacccaa cttccgcccg ccgatcaact catgatccgg caccagttcc gccaggtcga
      301 gtcgcagctc gatcgctcgg cgcggctggc ggcctcgatc ctctccgagt tgagcggcaa
      361 cctcgcgctg gtcacgccgc cccgcagccc gatcgagcgg ctgcgccact tcgagttgat
      421 cagcctgcgc gagcaggtcg cgctcctgat cgtggtgacc cagtccggcg ccgtccacca
      481 gatggtactg ccgctggagg agccgaccga acaaaccgac ttgagcgcgc tgagccagca
      541 gatcaacccg gaggtgcgca acgggaccgc cgctcaggtg ctgcagcggg cctccgagct
      601 gggcgggctg cccgcgctgg tgctgcagaa cgtggcggag gcgatcatgc gcgccgacca
      661 ggagagccag agcgcgctct tctccgacgg actggagcgc acgctggggc agcccgagtt
      721 cacgcgcagc gcggtggccc aacgtttgct cgaactgctg cggggcggga cgattctctc
      781 ggccctcctt ccccagttgg agttggaaga cgatgtgcaa gtattcatcg gatcggagaa
      841 caagtcggag gatctgcgct cgttcggcgt cgtcatctcg acctacggct ccggcgacga
      901 gatctccggg ctcctgggcg tgctggggcc aacgcgcatg ccgtacgggc gctcgatctc
      961 aaccgtccgc tacctggcgc aggtcttgag tgacttgttg ggaagcatct acgaggagag
     1021 cgcggacccc gccgagtagg gcgaggagga agcgaggacg acggactgat ggacgaagat
     1081 cgccgcgaat cggcgccgga gcagcaggcg cccgagaaga cgaccggcac cgacggcatg
     1141 ctcgacgagc agccggtcga gccggagacg atgagcacca cggtcgagca actggcagcc
     1201 gagcgggcac gcgccgagga gtatctggag caggcgcgcc gcgcgcgcgc cgagctgatc
     1261 aactaccggc gccgcagcga gcaggagatg gagcaggcgc ggcgcaacgc cggcgagcgc
     1321 atcatcgcgc gcctcttacc ggtggccgat gacttcgccc gcgccctcgg cgccgtgccg
     1381 gagagcgaga tgcagaaccc ctggatccag gggatcctgc tgatcgagcg caaactctgg
     1441 tcgattctcg aagcggaggg cgtgcagccg atcgaagcgg tcggccagcc cttcaacccg
     1501 gccgtccacg aggcggtcag catggagagc gggggcggca acgaggtcgt ggcggagtac
     1561 cagcgcggct acacgttgca cgaccgcgtg ctccggcccg cgatggtcaa agtcgggcag
     1621 ggcaaacaga acgggcaagc agccggctca gcgcagaacg gcgagccggc ctcgtagcag
     1681 tctcacagac gagtggaagg atcggtgcat ggcacggaca atcggaatcg acctcgggac
     1741 caccaactcg gtcatggccg tgatcgaggg cggcgagccg gtagtgctcc ccaacgccga
     1801 gggcgagcgg ctgaccccct cggtcgtggc ggtgaccaac accggcgagc gcctggtcgg
     1861 gcgcttcgcc aagcgccagg ccgtgacgaa cccctccaat accgtattct cggtcaaacg
     1921 cttcatgggc cgccgcttcg acgaccccaa cgtgcaggcc gatctcaagc ttgtacccta
     1981 tgaggtgcgt aaggcgcaaa acggcggtgt cgagatcaag atgggtgacc gttggtccag
     2041 cccgcccgag atctcggcca tgatcctgca gaagctgaag accgatgccg aagcctacct
     2101 gggcgacaaa gtcgatcggg cggtcatcac cgtgccggcc tacttcgacg acagccagcg
     2161 caacgccacc aaggacgccg gtcgcatcgc cggcctcgac gtgctgcgca tcatcaacga
     2221 gccgaccgcc tcggccctgg cctatggtct cgacaagaag aacgacgagc agatcgcggt
     2281 ctacgacctc ggcggcggca cctacgacat ctcgatcctc gatctctccg agggcgtctt
     2341 ccaggtgctg gccaccaacg gcgacaccca cctcggcggc gacgacttcg accagaagat
     2401 catcgactgg ctcgccgacg agttcaagaa gcaggagggg atcgacctgc gcggcgaccg
     2461 catggcgctg cagcgcctga aggaggccgc cgagcgggcc aagatcgagc tctccagcac
     2521 gatgcagacg gaggtcaccc tccccttcat caccgccgac cagagcggcc ccaagcacat
     2581 gaacatcacc ctctcccgct ccaagctgga gcaactggtg ggtgatctgg tcgaccgtac
     2641 cgtgcctccg atggactccg cgctcaagga cgcgaatctc agcaagggcg atgtcgacga
     2701 ggtgatcctg gtcggtggtc agacccgcat gccggcggtc cagcgcaagg tggccgattt
     2761 cttcggcaag gaagcgcaca aggggatcaa cccggacgag gtggtggcgg tcggtgcggc
     2821 cattcaggcc ggcgtgctgc agggcgaagt caaggatgtg ctcctgctcg acgtcacccc
     2881 gctgacgctc tcgatcgaga cgctgggcgg cgtggccacc ccgttgatcg agcgcaacac
     2941 gaccatcccg acgcgcaaga gccagatctt caccaccgcg accgacaacc agacccaggt
     3001 ggagatccac gtggtgcagg gcgagcgccc gatggccagc gagaaccgca gcctgggtcg
     3061 cttcatcctc gaccgcatcc cgcccgcgcc gcgcggcgtg ccgaagatcg aggtgacctt
     3121 cgacatcgac gccaacggca ttctgaacgt gaccgcgcgc gaccaggcga ccggccgcga
     3181 gcagaagatc accatcaccg cctccagcgg tctgaccgag gacgagatca agcgcatggt
     3241 gcacgaggcc gagtcgcacg cggaggacga ccgcaagcgg cgcgaggcga tcgagctgcg
     3301 caaccaggcc gaggcgatgg cctaccaggc cgagcgcacg ctggccgact acggcgaccg
     3361 catcccctcg gagctgaagg ccgagctgga caaccagatc caggccgtgc gcaccacgct
     3421 gcagcagaat cccgaggatg tcgagagcct acgcccggcc tacgagcagt tgacccagac
     3481 gctgtcgaag gtcggcgcca gcatgtacga gcagccggcc gcgggcggag ctcccggcga
     3541 ggagcagccc ggcgctcagc aagccggc
//
LOCUS       DASUBI010000426         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_695276, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000426 DASUBI010000000
VERSION     DASUBI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695276"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /gene="tcuA"
                     /locus_tag="VFU72_08180"
     CDS             <1..1453
                     /gene="tcuA"
                     /locus_tag="VFU72_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEU5423500.1"
                     /translation="NAAMCAALAAHEQGAEVLVLERAPEAEAGGNSRFTGGAIRFVYN
                     GLDDLLQVMPDLSPSEIENSDFGSYPREQFYADMERITQYRADPEMVGLLIDRSFDTL
                     VWMRQQGVRFVPSYQRQAFKVDGKFKFWGGLAVDASGGGPGLVENLTSAVRRAGIEIR
                     YETKAVALLTEDGQVRGVRVRHLGQEDDIHSRAVVLACGGFEANSEWRTRYLGPGWDL
                     AKVRGTRFNTGDGIRMALDIGAMPYGNWSGCHAVGWDRNSPEFGDLRVGDGFQKHSYP
                     FGIMVNANGQRFVDEGADFRNYTYAKYGHEVLKQPMQFAWQVFDSKVFHLYRDEYRIR
                     EVTKVRADTLEELAAKLDDVDPERFLETVRDFNAAVRTDIPFNPTVKDGRHTEGLAVP
                     KSNWANPLDTPPYEAYQVTCGITFTFGGLRITGDAQVVDSDGRPIPGLFAAGELVGGI
                     FYFNYPGGTGLTNGAVFGRIAGTSAGQLRSSTA"
     gene            complement(1429..2469)
                     /locus_tag="VFU72_08185"
     CDS             complement(1429..2469)
                     /locus_tag="VFU72_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEU5423501.1"
                     /translation="MIETSAGRAQLLRQLAPEYARVALDNIAREYPAAGMYVVDGPEP
                     LPLPRQRHPAFYGSYDWHSCVEMHWLLLRLLRLAPDDVPQAEVRAALDEHLAPEKLAG
                     ELAFFGQPRYRSVERPYGWAWLLALAYEAALLGDTDGHRWLENLTPLSDFFTTRLAEW
                     LPRLTYPVRYGVHPNTAFALTLALPFADARAAAGDARLHDATHAAARRWFSQDQDYPA
                     AWEPSGSDFLSPALTEALLMARLLEFEDFLPWIDRFLPGLAAGEPASLFTPAIVADAT
                     DGQTGHLHGLNLSRAWCFLRLAEALPPGDPRLPPMRQAAERHAAASLPEVVGGDYLLE
                     HWLAVYAVLLLS"
BASE COUNT          411 a          899 c          912 g          373 t
ORIGIN      
        1 caacgcggcc atgtgcgccg cgctggcggc gcatgagcag ggcgccgagg tgctggtgct
       61 cgagcgcgcg cccgaggccg aggcgggcgg caacagccgc tttaccggcg gcgccatccg
      121 cttcgtctac aacggcctgg atgacctctt gcaggtgatg cccgacctca gccccagcga
      181 gatcgagaac tccgacttcg gcagctaccc ccgcgagcag ttctacgccg acatggagcg
      241 tatcacccag taccgcgccg accccgagat ggtcggtctc ctgatcgacc gctccttcga
      301 cacgctggtc tggatgcgcc agcagggcgt gcgcttcgtg cccagttacc agcgccaggc
      361 cttcaaggtc gacggcaagt tcaagttctg gggcgggctg gcggtcgacg cctccggcgg
      421 cggccccggg ctggtcgaga acctgaccag cgccgtgcgc cgcgccggca tcgagatccg
      481 ctacgaaact aaggcggtcg ccctgctcac cgaggacggt caagtgcgcg gtgtgcgcgt
      541 gcgccatctg ggacaggaag acgacatcca cagccgcgcc gtcgtgctgg cctgcggcgg
      601 cttcgaagcc aatagcgagt ggcgtacccg ctacctcggc cccggctggg acctggccaa
      661 ggtgcgcggc acacgcttca acaccggcga cgggatccgc atggcgctcg acatcggcgc
      721 catgccctac ggcaactggt ccggctgcca cgccgtcggc tgggaccgca actcacccga
      781 gttcggcgac ctgcgcgtcg gcgacggctt ccagaagcat agttatccct tcggcatcat
      841 ggtcaacgcc aatggccagc gcttcgtcga tgagggagcg gatttccgca actacaccta
      901 cgccaagtat ggtcacgaag tgctgaaaca gccgatgcag ttcgcctggc aggtcttcga
      961 cagcaaggtc ttccacctct accgcgatga gtaccgcatt cgcgaggtga ccaaggtgcg
     1021 cgccgacacg ctggaggagc tggctgcgaa gctggacgat gtcgaccccg agcgcttcct
     1081 ggaaaccgtg cgcgacttca acgccgccgt gcgcaccgac atccccttca atcccacggt
     1141 caaggacggc cggcacaccg aggggctggc ggtgcccaag tcaaactggg ccaacccgct
     1201 cgacacgccg ccctacgagg cctatcaggt gacctgcggc atcaccttca ccttcggcgg
     1261 gctgcgcatc accggcgacg cccaggtggt cgactccgat ggccgcccga tccccggcct
     1321 cttcgcggcc ggcgagctgg tgggcggcat cttctacttc aactacccgg gcggcacggg
     1381 cctgaccaac ggcgcggtct tcggccgcat cgccggcacc tcagccggtc agctgaggag
     1441 cagcaccgcg tagacggcca gccagtgctc cagcaggtag tcgccgccga cgacctccgg
     1501 cagcgaggcg gcggcgtgcc gctcggccgc ctggcgcatc ggcggcaggc gcgggtcgcc
     1561 gggcggcagc gcctcggcca gtcgcaggaa gcaccaggca cggctgaggt tcagcccgtg
     1621 caggtgcccg gtctgcccgt cggtcgcgtc agccacgatg gccggggtaa agagcgaggc
     1681 gggctctccg gcggccagcc ccggcaggaa gcggtcgatc caggggagaa agtcctcgaa
     1741 ctcgagcagg cgagccatca gcagggcctc ggtcagtgcg ggcgagagga aatcgctgcc
     1801 cgagggctcc caggccgccg ggtagtcctg gtcctggctg aaccagcgcc gggcggcggc
     1861 gtgagtggcg tcgtgcaggc gtgcgtcgcc cgcggcggca cgagcgtcgg cgaaggggag
     1921 cgccagcgtc agggcgaagg cggtgttggg gtggacgccg tagcgcaccg ggtaggtcag
     1981 gcgcggtagc cactcggcca ggcgcgtggt aaagaagtcg ctcagcgggg tgaggttctc
     2041 caaccagcga tggccgtccg tatcccccag cagggccgcc tcgtaggcca gcgccagcag
     2101 ccaggcccag ccgtaggggc gctcgaccga gcggtagcgc ggctggccga agaaggccag
     2161 ctcgccggcc agcttctccg gcgccaggtg ctcgtcgagc gcggcgcgga cctcggcctg
     2221 gggcacatcg tccggcgcca gccgcagcag gcgcagcagc agccagtgca tctcgacgca
     2281 ggagtgccag tcgtagctac cgtagaaggc ggggtggcgc tggcgcggca ggggcagcgg
     2341 ctcggggccg tcgaccacgt acatcccggc ggccgggtat tcgcgcgcga tgttgtccag
     2401 cgccacccgg gcgtattccg gcgccagctg gcgcagcagc tgcgcgcgcc ccgcgctcgt
     2461 ctcgatcatc gccgtcgtct ctctccctgt ctatcagcga ctgctgggcg atggtagcat
     2521 gccgtcgtgt tgagtgcgtg gccgtcgagc agcctgggag ccgtcgagta cggatccttc
     2581 gactgcgctc aggat
//
LOCUS       DASUBI010000427         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1355995, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000427 DASUBI010000000
VERSION     DASUBI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355995"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="VFU72_08190"
     CDS             <1..1891
                     /locus_tag="VFU72_08190"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU5423502.1"
                     /translation="WAAAAGFLVGLMALAGHALTIVQIGYLLLAITALAAWRSGRGGR
                     REGVLIALLGLTPLVLGAGLAAVQLLPSLALSDVTERAGLAYQEASSASFMPHFALTF
                     IVPNFFSFDGPGKYWASGDLAETNGYVGLLPLLLAGLGIARAAPGRRRLIGLIACGTG
                     VALLLAFGSQTWLYRLAFELLPAVDRVRRPVDFIALAQLGVALLAAFGLAELERDRDG
                     AVLATLECWLGRALIAAAGAIILGALLLVNAAAEAHADLTALENGLILAGVLLLAAYL
                     AAHGARSWSISRQVLLTGLVVVAAFDLGSATAGKVYAGFQRSPGSYIGVDWAGDPADA
                     TVRTIQAAPSVAPARLYPTGVGSIWENGPLVWGLDSIDGYSVLWPTYYQDTFELATNA
                     PGSRLFALFDVRYVLAPDRPPRALSALSPDSVRTLRDQPPYLYELAGQGPRVWVASGA
                     VRLAGRDELGYLAAHDDELDRIITVSTKVPEGSTEPNASAPPGTAEITRYADDRVTIE
                     ARMSAPGFVVLDDTYFPGWQARIDGSPTRVLRADHTFRAVWVPAGEHQIDMRFSLPGL
                     RAGVLVSLVSLVLLLALAAYPSWRGRGRLVSLSWSRHAEDGGHAFGGEREESLYSQSQ
                     LSGPA"
     gene            1923..2756
                     /locus_tag="VFU72_08195"
     CDS             1923..2756
                     /locus_tag="VFU72_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5423503.1"
                     /translation="MICGKLSVVLPAHNELENLPGVVERALAVLPELTEGFELVVVDD
                     GSVDGTAALADELAATHPQITVVHHPRNRGYGAALTSGFEAASGDRIMFMDADRQFDI
                     ADLSYLAPFVDDYDIVAGYRVARQDAAYRRLYANMFKLAVRVLFGIRLRDIDCAFKVF
                     RADLLRDAKLESPGALINTEILAKARVRGASWVEVGVNHYPRAAGTSSGGSPKVVFRA
                     MRETILLWWRMRTYHPAPSDCSAAGTRPNLLPGAVIALVVTSLVAAGGLASRLARRRR
                     S"
     gene            complement(2751..3044)
                     /locus_tag="VFU72_08200"
     CDS             complement(2751..3044)
                     /locus_tag="VFU72_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU5423504.1"
                     /translation="MIALRETPEGTTFGVRVLPRAGKSGIERVEENELRMRLAAAPVA
                     GAANRALVALLADVLGCPRRDVEIVSGEHARRKVVRVRGLGAVAVEERLSASL"
     gene            complement(3041..>4207)
                     /locus_tag="VFU72_08205"
     CDS             complement(3041..>4207)
                     /locus_tag="VFU72_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEU5423505.1"
                     /translation="WGLTFDDPTFDGIQLCDNSCPFCFIKQLPRGMRKSIYVMDDDFR
                     YSMLYGSFVTLTNLTEEDWRRIEEQRLGPINVSVHATNPELRIALVGNPKAAQVMDDL
                     ARLERIGIDYNTQLVLCPGVNDGPELDRTIRDLLTLGPHLRSIAAVPVGLSKHGLERQ
                     SRRVRLSRTCMRTLPGKTIDVRRYAPQEALAVIEQAGAWQARFRAERGETFLYLGDEF
                     YLMTGLPVPPAEAYDGFPQVEDGIGITRLFLDDAAGVLARGAEGDVRGLAGVIACGTL
                     IAPTMIDLVERINARTGSRLRVAVVENTFFGSEINVSGLLTGALVRDALAADAERSPI
                     FISENMISKRTRTFLDDMDVAELSQVLGRPVLAAGYLSEVLRHLVRLQQTIDLR"
BASE COUNT          589 a         1425 c         1473 g          720 t
ORIGIN      
        1 ctgggccgcc gcggcgggtt tcctggtcgg cctgatggcg ctggccgggc atgcgctgac
       61 catcgtccag atcggctatc tgctgctcgc catcacggcg ctcgcggcct ggcggtcggg
      121 gcgaggcggc cgccgggaag gcgtactgat cgcgctgctc ggcctcaccc cgctggttct
      181 gggcgcgggc ctggcggcgg tccaactgtt gccttcgctc gcactcagcg acgtgaccga
      241 gcgcgccggg ttggcctatc aggaggccag cagcgcgtcc ttcatgccac acttcgcgct
      301 gacgttcatc gtgccgaatt tcttctcctt cgacggtccg gggaagtact gggcgtcggg
      361 cgacctggcc gagacgaacg gctatgtcgg actgcttccc ttgttattgg ccggactcgg
      421 gatcgcgcgg gccgcgcccg gcaggcgccg cttgatcggg ctgatcgcat gcgggaccgg
      481 agtggcattg ttgctggcct tcggctccca gacctggctc taccggctgg cgttcgaact
      541 gctgcccgcc gtcgaccggg tgcgtcgccc ggtcgacttc atcgccctgg cgcagctcgg
      601 agtcgcgctg ctggccgcct tcggcctggc ggagttggag cgcgatcgcg acggcgcggt
      661 gctcgcgacg ctggagtgct ggctgggccg ggcgctgata gcggctgcgg gagcgatcat
      721 cctgggtgcg ctcttgctgg tgaacgccgc agcggaggcg catgccgacc tgaccgcgct
      781 cgagaacggt ctcatattgg ctggggtgct gttgctggcg gcctacctgg cggcgcacgg
      841 cgcgcgcagt tggagcatct cacgacaggt gctgttgacc ggattagtcg tggtcgccgc
      901 cttcgatctc ggctcggcga cagccggtaa ggtctacgcc ggcttccagc gctcgcccgg
      961 ttcatacatc ggagtcgact gggctgggga ccccgccgat gccacagtgc ggacgataca
     1021 ggccgcaccg tccgtcgcgc ccgcgcggct ctacccgacc ggtgtcggct cgatctggga
     1081 gaacggtccg ctcgtttggg gtctggatag catcgacggc tacagcgtgc tctggccgac
     1141 ctactatcag gatacgttcg agctggccac caatgcgccc ggctcgcgac tttttgcgct
     1201 gttcgacgtg cgctacgtgc tggcgccgga tcggccgccg cgagcgctct ccgctctcag
     1261 cccggacagc gtccgaaccc tgcgcgacca gccgccctat ctctacgagc tggccggcca
     1321 gggaccgcgc gtctgggtgg cgagcggggc agtccggctg gccggccgcg acgagctcgg
     1381 ctacctggcg gcacatgacg acgagctcga tcggatcatt accgtgagca cgaaagtgcc
     1441 ggagggttcg actgagccga acgcctcggc gccgccggga accgccgaga tcactcgcta
     1501 tgccgacgac cgtgtcacga tcgaggcgag gatgagcgcg cccggcttcg tcgtgctcga
     1561 cgatacgtac tttcccggct ggcaggcccg gatcgacggg tcgccgacgc gggtgctgcg
     1621 tgcggaccat acgttccgcg ccgtctgggt gccggccggc gagcatcaga tcgacatgcg
     1681 gttcagtctg cccggtctgc gcgccggcgt gctggtcagc ctcgtgtcac tcgtcctgct
     1741 gcttgctttg gccgcctatc cgtcttggcg gggccgaggg cgacttgttt cgttgtcatg
     1801 gagcagacac gccgaagacg gcggtcacgc tttcggcggt gagcgcgagg aatcgctata
     1861 ctctcagtca caattgtccg gccccgcctg aatcgttggc tgggcccaga ggcgggggtg
     1921 tgatgatctg cggcaagctc tcggtggtgc tgccggctca caatgaactg gagaatctgc
     1981 cgggggtagt cgagcgtgcg ctcgccgtcc tgccggagct gaccgagggc ttcgagttgg
     2041 tcgtggtcga cgacggcagc gtcgacggca ccgccgcgct ggccgatgag ctggccgcca
     2101 cgcacccgca gatcacggtc gtccatcacc cgcgcaaccg gggctacggc gcggcgctga
     2161 cctcgggttt cgaagcggcc agcggcgatc gcatcatgtt catggacgcc gatcgccagt
     2221 tcgacatcgc ggatctgagc tatctggcgc ccttcgtcga cgactacgac atcgtggccg
     2281 gctatcgcgt ggcgcggcag gatgccgcct accgtcggct ctacgccaat atgttcaaac
     2341 tggccgtgcg cgtgctcttc ggcattcgac tgcgggacat cgactgcgcg ttcaaggtct
     2401 ttcgcgccga tctgctgcgc gacgcgaagc tggagtcgcc gggagccctg atcaacaccg
     2461 agatcctggc caaggcgcgc gtgcgcggcg caagctgggt cgaggtgggg gtcaaccact
     2521 acccgcgcgc cgccggcaca tcttcgggcg gcagccccaa ggtcgtcttc cgcgccatgc
     2581 gcgagacgat ccttctctgg tggcgcatgc gcacgtatca ccctgcgccg tccgactgct
     2641 ccgccgctgg cacccggccg aacctcctcc cgggcgccgt aatcgcgctg gttgtcacct
     2701 cgctggtcgc cgcgggtggg ctcgcctcgc gcctggctcg acgccgccgc tcatagcgag
     2761 gcgctgagcc gctcctcgac cgcgacggcg cccagaccgc ggacccgcac caccttccgc
     2821 cgcgcgtgct cgcccgacac gatctcgaca tcgcgacgtg ggcagcccag cacgtctgcc
     2881 agcagcgcca ccagcgcccg gttggccgcg cccgcaaccg gcgcggcggc cagccgcatg
     2941 cgtagctcgt tctcttcgac gcgctcgata ccgctcttgc cggcgcgtgg caggacacgc
     3001 acgccgaagg tcgtgccctc gggcgtctcg cgcagcgcga tcaccgcagg tcgatggttt
     3061 gctggagccg caccaggtgg cgcagcacct cactcagata gccggcggcc agcacggggc
     3121 gcccgagtac ctggctcagc tcagcgacgt ccatgtcgtc gaggaaggta cgggtgcgct
     3181 tggagatcat gttctcggag atgaagatcg gagagcgctc cgcgtcggcg gccagcgcat
     3241 cgcgcaccag cgcgccggtc aacagaccgg agacgttgat ctcgctgccg aagaaggtgt
     3301 tctcgaccac ggccacgcgc agccgtgagc cggtgcgcgc gttgattcgc tcgaccagat
     3361 cgatcatggt cggggcgatc aacgtaccac aggcgatcac gccggccagg ccccgcacat
     3421 cgccctcggc gccgcgcgcc agcacgcctg cggcgtcgtc caggaagagg cgggtgatgc
     3481 cgatgccgtc ctccacctgc gggaagccgt cgtaggcctc ggccggcggc accggcaggc
     3541 cggtcatcag gtagaactcg tcgcccaggt agaggaaggt ctcgccgcgc tcggcgcgga
     3601 agcgcgcctg ccacgccccc gcctgctcga tgaccgccag cgcctcctgc ggcgcgtagc
     3661 ggcgcacgtc gatcgtcttg ccggggaggg tgcgcatgca ggtgcgcgag aggcgcacgc
     3721 ggcggctctg gcgctcgagg ccgtgcttgg agagacccac cggcaccgcg gcgatcgacc
     3781 gcaggtgcgg gccgagcgtc agcaggtcgc ggatggtgcg gtccagctcg gggccgtcgt
     3841 tgacgccggg gcagagcaca agttgagtgt tgtagtcgat gccgatgcgc tccagccgcg
     3901 ccaggtcgtc catcacctga gcagccttgg ggttgccgac cagggcgatg cgtagctcgg
     3961 ggttggtggc gtgcaccgag acattgatcg ggcccaggcg ctgctcctcg atacgccgcc
     4021 agtcctcctc cgtcaggttg gtcagcgtca cgaagctgcc gtagagcatc gagtagcgga
     4081 agtcgtcgtc catgacgtag atgctcttgc gcatccctct ggggagctgc ttgatgaagc
     4141 agaaggggca ggagttgtcg cagagctgga tgccgtcgaa ggtgggatca tcgaaggtga
     4201 ggcccca
//
LOCUS       DASUBI010000428         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1349197, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000428 DASUBI010000000
VERSION     DASUBI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349197"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFU72_08210"
     CDS             complement(<1..233)
                     /locus_tag="VFU72_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423506.1"
                     /translation="MSARGIRVLIVDDVAESRDNVERLLRFEPDVQIVGKASRGQEGI
                     DLAFRLNPDIILMDINMPDMDGVTATREITSRNP"
     gene            complement(233..1210)
                     /gene="cpaB"
                     /locus_tag="VFU72_08215"
     CDS             complement(233..1210)
                     /gene="cpaB"
                     /locus_tag="VFU72_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU5423507.1"
                     /translation="MRKGGRLFIVVGIALALVAAMLAVTAFRGSDKKSSASAEKQVTV
                     LEAAHDLKASTVLRAEDIQTAQVAASSAPKNVLSSPNQAIGLATSGDLVKGQQIVADN
                     LVTPGLTNTISNGKRAVALPVDRVSALGGMIRAEDYIDIVYSTRIQLNRILSTNIAEE
                     REGNNDFKFQDGTLQVPPPGTQPPANYPYPGEDGSRFVVTDGGNGNEVTKVILQNVKV
                     LQVIAGDVALAPPADASSTPTSNNKNDATKLPNADLLVLEVDPQQAEIIKFLQDTSSG
                     QQGTVSYQVALRSADDHAAATTTGMTYDQLMTTYGLPAPKSVHLPGGGH"
     gene            1416..>2407
                     /locus_tag="VFU72_08220"
     CDS             1416..>2407
                     /locus_tag="VFU72_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5423508.1"
                     /translation="MDHDIQALLYGADSTERIVAVEPRGGSAVAVYRRTDTGTLHVEQ
                     ELFSPWLLTTAAGADEIGGRAGVVVLDGDLPLAYRLNFRSWRDELDARDRLRERGIPL
                     HRLGSPVDQYLAASGRTLFKGMQFDDLVRLQLDIETTSLEATGEGAAIIAIALSSNSG
                     YEEVLDGGDLSEGELLQALTGRIRSLDPDVIEGHNIFNFDLPFLLERARHHGLHLTWG
                     RDGSTPRLHGTQRFKAGARVIPYLGASVAGRHLIDTYQQIQRYDYSGEMASYGLKEAV
                     ESLGLTRAGRVFVPGAAITGTWQHDRERVLAYALDDVRDVALLSRLAVPTEFYQA"
BASE COUNT          364 a          813 c          785 g          445 t
ORIGIN      
        1 gggttgcgcg aggtgatctc ccgcgtcgcg gtgacaccat ccatgtcggg catgttgatg
       61 tccatcagga tgatgtccgg gttgagccgg aaggcgagat cgatgccctc ctgaccgcgc
      121 gaggccttcc ccacgatctg cacgtccggc tcgaatcgca gcagccgctc gacgttgtcg
      181 cggctctccg cgacgtcatc gacgatcagt acccggatgc ctcgcgcact cactagtgac
      241 ccccacccgg caggtgcacc gacttcggcg ccggcagccc gtaggtcgtc atcaactggt
      301 cgtaggtcat gccggtggta gtcgccgccg cgtgatcgtc ggcgctgcgc agcgctacct
      361 gataactgac cgtgccctgc tgccccgatg atgtatcctg caggaacttg atgatctcag
      421 cctgttgcgg gtccacctcc agcaccagca ggtccgcgtt cggcagcttc gtggcgtcgt
      481 tcttgttgtt cgaggttggg gtgctgctgg catccgccgg cggcgccagc gccacgtcac
      541 ccgcgatcac ctggagcacc ttgacgttct gcaggatcac cttggtgacc tcgttgccgt
      601 ttccgccgtc cgtgaccacg aagcgcgagc cgtcctcgcc ggggtagggg tagttcgcgg
      661 gcggctgcgt accgggtggc ggcacctgca gcgtgccgtc ctggaacttg aagtcgttgt
      721 tgccttcgcg ctcctcggcg atgttggtgg agaggatgcg gttcaactgg atgcgcgtgg
      781 agtagacgat atcgatatag tcctcggctc gaatcatgcc cccgagcgcg ctgacgcgat
      841 cgaccggcag cgcgacagcc cgcttgccgt tcgagatggt gttggtcagc ccgggcgtca
      901 ccaggttatc ggccacgatc tgctggccct tgaccagatc gcccgaggtc gccaggccga
      961 tcgcctggtt cggactggag agcacgttct tcggcgcgga cgaggcggcg acctgggctg
     1021 tctgaatgtc ctcggcgcgc agcaccgtgc tggccttgag atcatgcgcg gcttccaaca
     1081 ccgtcacctg cttctccgcg ctggcggagg acttcttgtc gctccctctg aacgcggtaa
     1141 ccgccagcat cgccgcaacc agggccagag cgatccccac gacgatgaag agacgtcctc
     1201 ctttgcgcat gccagattcc tccctgatgc gtccgctgcc gcagtttgcc gtcgtgcggg
     1261 agtacaatta cgcgcgattt gcttcactcc cgatggtgag tctagggagc agatgggcta
     1321 gtcaccatag gacgttggtc accgccccaa ctacaaggag tcccgtttca acgccgcatc
     1381 tgctcgtcgc ggtgcgctgg agagtcgtct caggcatgga ccatgacatt caggcactcc
     1441 tctacggcgc cgattccacc gagcgcatcg tcgcggtcga gccgcgcggc ggctcggcgg
     1501 tcgccgtcta ccgccgcacc gatacgggta cgctccacgt ggagcaagag ttattcagtc
     1561 cctggctgct gacgacggcg gccggggccg atgagatcgg cggccgggcc ggcgtcgtgg
     1621 tgctcgatgg cgatctgccg ctcgcctacc gcctcaactt ccgctcctgg cgcgacgagc
     1681 tcgacgcgcg cgatcgcctg cgcgagcgcg gcatcccgct ccaccgcctg ggcagcccgg
     1741 tcgatcagta tctggccgcc agcggacgca ccctcttcaa ggggatgcag ttcgacgacc
     1801 tggtgcgcct gcagctcgat atcgagacga ccagtttgga ggccaccggc gagggcgccg
     1861 cgatcatcgc gatcgcgctc agctccaatt ccggctacga ggaggtgctg gacggcggcg
     1921 acctcagcga gggcgagctg ctgcaggcgc tgaccgggcg cattcggtcg ctcgatccgg
     1981 atgtgatcga ggggcacaac atcttcaact tcgacctgcc gttcctgctg gagcgggccc
     2041 ggcatcacgg cctgcacctg acctggggcc gggacggaag tactcctcgg ctgcacggca
     2101 cacagcgctt caaggccgga gcgcgggtca tcccctacct cggcgcctcg gtcgccgggc
     2161 ggcacctgat cgacacctac cagcagatcc agcgctacga ttactccggc gagatggcca
     2221 gctacgggct gaaggaggcg gtcgaatcgc tcgggctcac ccgtgccggg cgggtcttcg
     2281 tgcccggcgc ggcgatcact ggcacctggc agcacgaccg cgagcgcgtg ctcgcctacg
     2341 cgctcgacga tgtgcgcgac gtggccctcc tgagccgcct ggcggtgccg accgagttct
     2401 atcaggc
//
LOCUS       DASUBI010000429         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1212130, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000429 DASUBI010000000
VERSION     DASUBI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212130"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VFU72_08225"
     CDS             <1..1520
                     /locus_tag="VFU72_08225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEU5423509.1"
                     /translation="AVEEQFYLFWPLLVVLGLRGSRRRLLALSLTGALASLGLMLWLY
                     HPGADPSRVYYGTDTRALELLVGAALACLRPPGHERPAPRAARRAALLLDLAGIALFG
                     SLLAACRLVHQYDAWLYRGGMALLALATTGLIAIVAHSANCLVARLLALRPLRWLGRR
                     SYAIYLWHWPILMVTRSGLDVPLSGLPLLGLQVAATLAIAELSFRLVEEPVRHGALGR
                     IWRSLREDWRGRRRRRLALWAGTGMSASLALLTLGTLTTEARPPQPPAYLAVQAIHSV
                     PAAPAAATPGAPSPPTATPAPRPTPLVLPTPERWTVAIATPTVVATPTPASTPAPTPL
                     PTPTLTSTPAVATVPASLRVTALGDSVMVGAADALRQVIPNIGIDAVEGRQADELIAV
                     ARQLRDEGRLGDVVVVHLGSNGGVAPDQFDTLLRLLQGVPRVIVLNAKVPREWEAPNN
                     AVIARGVARYPNAELLDWHRASSDHPEFFYDDGSHLRPDGAAFYAHLIASAIDGG"
     gene            complement(1732..>2146)
                     /locus_tag="VFU72_08230"
     CDS             complement(1732..>2146)
                     /locus_tag="VFU72_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423510.1"
                     /translation="GLAFPAVAALLTSGAALGLIGVVAGLPNRHALARPALALVTAAG
                     LWLFWRAGPLAAAGLAAGCAAGLILLAGAIDSPGERLGFRLPAATFWFTWGMLLQVAV
                     LFAYYTFTGSPPAAAVAVALFLLGALAPTLTQIRG"
BASE COUNT          278 a          811 c          764 g          293 t
ORIGIN      
        1 tggcggtcga ggagcagttc tacctctttt ggccgctgct ggtcgtgctc ggcctacgcg
       61 gctcacgtcg ccggctgctg gcgctcagcc tgaccggcgc gctcgcctcg ctcgggctga
      121 tgctctggct ctaccacccc ggcgccgacc cctcgcgggt ctactacggc accgacacgc
      181 gcgcgctcga gctgctggtc ggcgcggcgc tggcctgcct gcggccgccc ggccatgagc
      241 ggcccgcacc ccgagctgcc cggcgcgcgg cgttgctgct cgatctcgct gggatcgcgc
      301 tgttcggcag cctgctggcc gcgtgccggc tcgtgcatca gtacgacgcc tggctctacc
      361 gcggcgggat ggcgctgctg gcgctggcca ccaccgggct gatcgcgatc gtggcgcact
      421 ccgccaactg cctcgtcgcg cgcctgctcg cgctcaggcc gctgcgctgg ctcggccgtc
      481 gctcctacgc catctatctc tggcactggc cgattctgat ggtcacacgg tcggggctcg
      541 acgtgccgct gagcggactg ccgctgctgg gtctgcaggt cgccgccacg ctggcgatcg
      601 ccgagctctc cttccggctg gtggaggagc cggtgcgaca cggcgcgctc gggcgaatct
      661 ggcgctccct gcgggaagac tggcgcggcc ggcgccgccg gcgcctggcg ctctgggccg
      721 ggaccggcat gagcgcgagc ctggcgctcc tgacgctcgg caccttgacg accgaggctc
      781 gcccgcccca gccccccgcc tatctcgcgg ttcaggccat ccacagcgta ccggccgcgc
      841 cggccgcggc aacgcccggc gcccccagcc cgcccaccgc cacccccgcg ccccggccca
      901 caccgctcgt gctgccgacc cccgagcgct ggacggtggc catcgccaca cccacggttg
      961 ttgcgacgcc tactccggcc tccacgccgg ctccgacacc gttgcccacg cccacactga
     1021 catcgactcc ggccgtggcg acggtgcccg cctcgctccg tgtgaccgcg ctgggcgact
     1081 cggtgatggt cggcgccgcc gatgccctac gccaggtgat ccccaacatc ggcatcgacg
     1141 cggtggaggg acggcaggcc gacgagttga tcgcggtcgc gcggcagttg cgcgacgagg
     1201 ggcggctcgg cgacgtcgtg gtcgtgcacc tcggctcgaa cggcggggtg gcgcccgacc
     1261 agttcgatac gctgctgcgg ctcttgcagg gcgtgccgcg ggtgatcgtg ctcaacgcta
     1321 aggtgccgcg cgagtgggag gcgccgaaca acgccgtgat cgcgcggggc gtggcgcgct
     1381 accccaacgc ggagctgctc gactggcacc gcgccagcag cgatcatccc gagttcttct
     1441 acgacgacgg cagccacctg cgtccggacg gcgcggcctt ctacgcccac ctgatcgcct
     1501 cggccatcga tggcggctag ccgctgcctg acaggcatgg gtgtcatccg ggacgaatcc
     1561 ctccacttcg gccgggatga cagggtgagg aaggggctgg cgaagacgac gcgccctcgt
     1621 cgtacgcctc atccttaccc tgtcatccct tcggcaggct cagggcaggc tctgagcgca
     1681 gtcgaaggat ccgtgcccca cgcgccaccc gacggattcg acaaccacgc gctagccgcg
     1741 gatctgcgtg agcgtgggcg cgagcgcgcc gaggaggaag agggcgacgg cgacggcggc
     1801 ggccgggggc gagccggtga aggtgtagta ggcgaagagc acggccacct gcaggagcat
     1861 gccccaggtg aaccagaagg tggcagcagg caatctgaag cccaggcgct cgccgggcga
     1921 gtcgatcgct ccggccagca ggatcagccc agccgcgcag ccggcggcca gcccggccgc
     1981 cgccagcggg ccggcccgcc agaagagcca gaggcccgcg gcggtcacga gcgccagggc
     2041 cgggcgcgcc agcgcgtgcc ggttgggcag gccggcgacg acgccgatca gcccgagtgc
     2101 ggcgccgctg gtcagcagcg ccgcgaccgc cgggaacgcg agaccg
//
LOCUS       DASUBI010000430         9037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_628113, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000430 DASUBI010000000
VERSION     DASUBI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628113"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFU72_08235"
     CDS             complement(<1..627)
                     /locus_tag="VFU72_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5423511.1"
                     /translation="MAESEQILTLPGDDPSRGAPGEGTNPDRVAVVGAGPNGLAAAIT
                     LAQVGLPVTLYEAGATVGGGTRTAELTLPGFRHDICSAIVPLALASPFFSRLSLETFG
                     VRFIQPPIPLAHPFDDGSAAVLARALEETAASLGPDAAAYAALMRPLLAEAGKLLADI
                     LSPLRLPHHPLLLARFGWPALRSAAGLAGARFQGERARALFAGLAAHSM"
     gene            complement(620..865)
                     /locus_tag="VFU72_08240"
     CDS             complement(620..865)
                     /locus_tag="VFU72_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423512.1"
                     /translation="MSIDRLALRARQIRALYQRELTALHRDPSLSAADMAAHEAALWR
                     QTAEQLASLRREAEQDGALSDEARALFAPPAPGGDDG"
     gene            complement(862..2088)
                     /locus_tag="VFU72_08245"
     CDS             complement(862..2088)
                     /locus_tag="VFU72_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423513.1"
                     /translation="MRSIGGRAVARTEAVESVERIELLPLAVLCLVVLLIYASTYLLL
                     AVLPIHIRALVGSESTVGTIMSLFSLAALLVRPFAGLLSDRVGRRPSIFAGALLIGAS
                     CVGYAVAGAHTIFLARILNGLGWGAVTAATATVAADLAPPSRRGFVLGIYGTVTGLAL
                     AVGPAAGTWLAGRLSLTTTFGIAAACGFGAAAGILLLHAPSPPRPASSRFGLHHLFTR
                     QALGPAAIMLCFTLMYGTVLTFIPLYAIDRHLGSSGPFFSVFAVVLIVMRVTGGRLSD
                     RWGRFAVIAPGFVFAGAAMVLLAGAQQRALLLLAAVLFAVAFALVQPAAQAWAVDRSP
                     AELRGTTLSTVVAAQDLGISLGTVAAGFTADAVGYGGLFLALTAVAVVGLAAAWLVFR
                     ATGQPLRAARPGAGRA"
     gene            2387..3220
                     /locus_tag="VFU72_08250"
     CDS             2387..3220
                     /locus_tag="VFU72_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5423514.1"
                     /translation="MDGTEGRLAVPDGELYYRIDDFTDPWRESETILLVHSAAGNVER
                     WRGWIPTLARRYRVLRFDLRGHGRSSTPPPDYPWSIARLARDIDDLLNELGTGPVHYV
                     GNSAGGIIGLQFAHDYPEQVRSLTLIGATPQLSRAKVSLAEWIERTERLGVRGFFRSD
                     AATRFSPQADPGLIEWFADETAKTPQSVVTSFIPYMNSLDLTSLLPGIEAPTLLLAAE
                     FDQITPAEVQREMQAAIPFARLVFYPTSGHNIGDEFPDRCAADTLGFIDELSAMKGGA
                     R"
     gene            3217..4026
                     /locus_tag="VFU72_08255"
     CDS             3217..4026
                     /locus_tag="VFU72_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEU5423515.1"
                     /translation="MSGGKPFPVLLGFDVDGETLWTARDPANAARPVTLSQGRYGPEV
                     GVPRLLDLLKRQGIQATFFVPGMVIERYADLMRRLVDEGHEIAHHGYSHKWPDSMSPE
                     EEREDFDKALAAFDSVLGRRPVGYRSPAWEFSPWTMNLLLEHGIKYSSNFMDRDSPYK
                     HVVDGRPTELVELPVQWLLDDAPFWLYSTRLPGRQIAGPETVLQTWWQEFDALYAERE
                     RGACFVLAMHPQISGRPYRVKLLERLIHHMKEYDVRFYRGDEAAAALGAGL"
     gene            complement(4039..5301)
                     /gene="larC"
                     /locus_tag="VFU72_08260"
     CDS             complement(4039..5301)
                     /gene="larC"
                     /locus_tag="VFU72_08260"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEU5423516.1"
                     /translation="MPVAYFDPFSGASGDMILGALVDAGLALDDLLGMLAGLDLSGYE
                     LAAERVTQHGVSGTRVSVRVAGEQQARSWRDIRALIERAALPDPVRQHALAVFAALAE
                     AEGRVHGVPADAVHFHEVGAVDSIVDVVGAAAGLQLLGVEAVYCGPLSLGSGFVSTRH
                     GLLPVPAPATAQLLAASGAPTVAREIEAELLTPTGAAVLTRLARFERPALHASAIGYG
                     FGQRELPWPNALRLWLGELDPAAAPPGDDSGPSELLLETNIDDMNPEFYEPLIERLFA
                     AGALDVFLTPIIMKRGRPATQVSVICAAADRAELDRVLIENSSTLGVRALPLDRTKAG
                     RRVEEVVTRWGEVRVKLKIWRGRVLDAVPEYADCLAVAHETGMPIRYIYGEASRIAEA
                     YVGRRMDESSPSPRIGRGSRGGEGSSSG"
     gene            complement(5310..6437)
                     /gene="rlmN"
                     /locus_tag="VFU72_08265"
     CDS             complement(5310..6437)
                     /gene="rlmN"
                     /locus_tag="VFU72_08265"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5423517.1"
                     /translation="MTIDLTTTDAPRTAPAARPLRRRAERPVTLYDLTLPDLEARLGE
                     LGLPRYRARQLYHWAYQQLAPDYATMTVLPKTLRAELAERLPFSQLTPVRQAVSDDGE
                     TVKLLLRTFDGEHIETVLMFYPDRTTVCVSCQVGCAVGCAFCATGMMGLTRNLTAGEM
                     VAQVIEAARAARARGRTLTNIVMMGMGEPFQNYANVLKMIAILHDEAGLNFGSRRTTV
                     STSGLVPFIDKLAEEPYQVKLAISLHAPNDELRGQLVPLNRRYGVAELMAACRRYVAR
                     TKRRVTFEYALIKDVNDSDAIARELAALLKGLLCHVNVIPLNPTPAAPFERPSVERIE
                     RFAALLREAGIPATVRYSRGVDIAAACGQLRVEHEQQAATA"
     gene            6688..7506
                     /locus_tag="VFU72_08270"
     CDS             6688..7506
                     /locus_tag="VFU72_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423518.1"
                     /translation="MRKTLRWLGLITLMLVTLACSQQSVSSPPPAAQFAATTAIAGAT
                     PTPAPTVTPAPTATTPPGTPKPAATAAPTVAPTPNPNATWVPLNAIDQDLSHGLLRET
                     DLDKTWSKQDAVDHTFVMALNQINCDGAQLTEPVPPQRSAEIAYQRDAGYLPEPLLIQ
                     LNWALPSDDAADTDMDFIKQALRCSNWENASPGAAVGSVDIRSYDLAKLGDDSVAARM
                     NISLNGTALVLTSAYVRSGSVITNVVYVDTKPADPKEFEALAKKAIQRAASLHL"
     gene            7774..>9037
                     /locus_tag="VFU72_08275"
     CDS             7774..>9037
                     /locus_tag="VFU72_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5423519.1"
                     /translation="MADAMVERLRANLQAAGIEVDEADLNGMVEKGFLRSIQAAEEAV
                     STFSVELVPDYLKPWGDVAAEYSSPPRESLTIPHIPPRPGFPSLVELAEHIHTREVSP
                     VEVTRGVLERIAQRDPALNAFQVVLADEALAAAREAEREIAAGHVRSPLHGVPLAVKD
                     LYAMRGTVTTAGSQILGDWRTDFDAAVVERLRDAGAVIVGKTRLSEFAYSPGSNNAHY
                     GPTRNPWNLEHDTGGSSSGSAAAVADGLVYGALGSDTGCSIRTPASLCGIVGIKPTFG
                     RVSLFGGVTLSWSLDHAGPMTRTVADGAVMLAILAGYDARDIRTRPVPVPDYAATLNE
                     GVRGLRVGVLRDDGSGEPLGTPEVLAAWRAGVAALDAAGAELIEIDLPQLHELRSINS
                     AIIAAEAAAFHQPWLQERLADYGEFPRQR"
BASE COUNT         1401 a         3198 c         3204 g         1234 t
ORIGIN      
        1 catcgagtgc gccgccagcc cggcgaagag ggcgcgagcg cgctcaccct ggaagcgggc
       61 accggccagc ccggcggccg agcgcagggc cggccagccg aagcgcgcca gcaagagcgg
      121 gtggtgcggc agccgcaggg gactgaggat gtcggccagc agcttgccgg cctcggccag
      181 caacggccgc atcagcgcgg cgtaggcggc ggcgtcgggg ccgagtgagg cagccgtctc
      241 ctcgagcgcg cgcgccagca cggcggcgct gccgtcgtcg aaggggtgcg ccagagggat
      301 aggcggctgg atgaagcgca cgccgaaggt ctccagggac agccggctga agaacggcga
      361 ggcgagcgcc agcggcacga tggccgagca gatgtcgtgc cggaagccgg gcagggtcag
      421 ttcggcggtg cgcgtgccgc cgcctaccgt ggcgcccgcc tcgtagaggg tcaccggcag
      481 accgacctgg gccagcgtga tcgccgccgc cagcccgttc ggccccgccc ccaccaccgc
      541 gacacggtca ggattcgtcc cctctcccgg tgcgccacgc gaggggtcgt ctcctggcag
      601 agtgaggatc tgctccgact cagccatcgt cgcctcccgg tgccggcggt gcgaagaggg
      661 cgcgcgcctc gtcgctcagc gcgccgtcct gctcggcctc gcgccggaga ctcgccagtt
      721 gctcggcggt ctgccgccag agcgcggcct cgtgcgccgc catgtccgcc gcgctcaggc
      781 ttgggtcgcg gtgcagcgcg gttagctcgc gctggtagag cgccctgatc tgccgcgcgc
      841 gcagcgccag ccggtcgatg ctcatgctcg gccggcaccc gggcgcgcag cccgcagcgg
      901 ctggccggtg gcgcggaaga ccagccaggc ggctgccaaa cccaccacgg ccacggcggt
      961 cagcgccagg aagagaccac cgtagccgac cgcgtcggcg gtgaagccgg cggccacggt
     1021 gccaaggctg atgccgagat cctgcgccgc caccaccgtc gacagggtcg tgccgcgcaa
     1081 ttcagccggc gagcgatcga ccgcccaggc ctgcgccgcc ggctgcacca gcgcgaaggc
     1141 gacggcgaag aggaccgccg ccagcagcag cagcgcacgc tgctgcgcgc cagccagcag
     1201 caccatggcc gcgcccgcga agacgaagcc gggcgcgatg accgcgaagc ggccccagcg
     1261 gtccgacagc cgtccgccgg tgacgcgcat cacgatcagc accacggcga agacgctgaa
     1321 gaagggcccg gaggagccga ggtggcggtc gatggcgtag agcggtatga aggtgagcac
     1381 ggtgccgtac atcagggtga agcagagcat gatcgccgcc gggccgagcg cctggcgggt
     1441 gaagagatgg tgcaggccga agcggctgct cgccggccgc ggcgggctgg gcgcgtggag
     1501 cagcaggatg ccggcggcgg cgccgaagcc gcaggccgcc gcgatgccga aggtggtcgt
     1561 caggctgagc cgcccggcca gccaggtgcc ggccgccgga ccgaccgcca gcgccaaccc
     1621 ggtgacggtg ccatagatgc cgagcacgaa gccgcgccgg ctcggtgggg cgagatcggc
     1681 ggcgacggtg gcggtggcgg cggtcaccgc gccccagccg aggccgttca ggatgcgcgc
     1741 caggaagatg gtgtgcgccc cggccacggc gtagccgacg caactggcgc cgatcagcag
     1801 cgcgccggcg aagatcgagg ggcggcggcc gacgcggtcc gacagcagcc cggcgaaggg
     1861 ccgcaccagc agcgcggcca gcgagaagag gctcatgatg gtgccgacgg tcgactcgct
     1921 gccgaccagc gcacggatgt ggatgggcag gaccgccagc agcaggtagg tgctggcgta
     1981 gatcagcagc acgaccaggc agagcaccgc gagcggcagc agttcgatgc gctcgacgct
     2041 ctcgacggcc tcggttcgcg cgacggcccg accccctatg gacctcatgg atggcccctg
     2101 cccgttgacg tcgctgcgtg gcgcgtcacg tcggatcctt cgacaagctc aggatgacag
     2161 ggtaagagag gggcctaccg gtagaggatg atcgtcgcca agacccatcc tcatcgtcag
     2221 cccctcactc tctctgtcat cctgagcttg tcgaaggatc cgttctagtg cggcacccaa
     2281 cctactcgtt cgcccccttg ccggcccatt gtaacgaacg tcgtgcgtgc tgacccgttt
     2341 tggtctacgc tgtcgcgatc acgcggcaaa cgggaaggag gagccggtgg atgggactga
     2401 aggcaggctg gcggtacccg atggagagct ctactaccgc atcgacgatt tcacggaccc
     2461 ctggcgcgag agcgagacga tcctcttggt gcactcggcg gccggcaacg tcgagcgctg
     2521 gcgcggctgg atccccactc tggcgcgccg ctatcgggtg ctgcgcttcg acctgcgcgg
     2581 ccacgggcgc tccagcaccc cgccgcccga ctacccctgg tcgatcgcgc ggctggcgcg
     2641 cgacatcgac gacctcctga atgaactcgg caccggcccc gtccactacg tcggcaactc
     2701 ggccggcggc atcatcgggc tccagttcgc ccacgactac ccggagcagg tgcgcagcct
     2761 gacgctgatc ggcgccacgc cgcagctcag ccgcgccaaa gtcagcctag ccgagtggat
     2821 cgagcgcacc gagcggctgg gcgtgcgcgg cttcttccgc tccgatgcag ccacccgctt
     2881 cagcccgcag gccgatccgg gcctcatcga gtggttcgcc gacgagaccg ccaagacgcc
     2941 gcagtcggtg gtcaccagct tcatccccta catgaacagc ctcgacctga cgtccctgct
     3001 gccggggatc gaagcgccca cgctgctgct ggccgccgag ttcgaccaga tcacgccggc
     3061 cgaggtgcag cgcgagatgc aggctgccat tcccttcgcc cgcctggtct tctaccccac
     3121 cagcggccac aatatcggcg acgagttccc cgatcgctgc gccgccgata cgctgggctt
     3181 cattgacgaa ctctccgcca tgaagggagg cgcccgatga gcggcggcaa gccgttcccg
     3241 gtcctgctcg gcttcgacgt cgacggcgag acactctgga cggcgcgcga cccggccaac
     3301 gccgcccgcc cggtgacgct ctcgcagggg cgctacgggc ccgaggtcgg cgtgccgcgc
     3361 ctgctcgacc tcctcaagcg gcaaggcatc caggccacct tcttcgtgcc cggcatggtt
     3421 atcgagcgct atgctgacct gatgcggcgc ctggtggatg aaggtcacga gatcgcccac
     3481 cacggctact cgcacaagtg gcccgacagt atgtcgccgg aggaggagcg cgaggacttc
     3541 gacaaggccc tggcggcctt cgacagcgtg ctgggcaggc gcccggtggg ctaccgctcg
     3601 ccggcctggg agttcagtcc ctggaccatg aacctgctct tggagcacgg catcaagtac
     3661 tcctccaact tcatggaccg cgactcgccc tacaagcacg tcgtcgacgg acgcccgacc
     3721 gagctggtgg agctgccggt gcagtggctg ctggacgacg cgcccttctg gctctacagc
     3781 acgcgcctgc cggggcggca gatcgccggg ccggagacgg tcttgcagac ctggtggcag
     3841 gagttcgacg cgctctacgc cgagcgcgag cgcggcgcct gcttcgtgct ggcgatgcac
     3901 ccgcagataa gcgggcggcc ctaccgggtc aagctgctgg agaggctgat ccaccatatg
     3961 aaggagtacg acgtgcgctt ctaccgcggc gacgaggcgg cggcggcgct gggggcgggg
     4021 ctgtaggcgg acgagcgctt accccgacga ggatccctca ccccccctgc tccctctccc
     4081 aattctggga gagggggagc tttcgtccat gcggcggccg acgtaggctt cggcgatgcg
     4141 gctggcctcg ccgtagatgt agcggatcgg catgccggtc tcgtgggcga cggccaggca
     4201 gtcggcgtac tcgggcacgg cgtcgagcac gcggccgcgc cagatcttca gcttgacgcg
     4261 cacctcgccc cagcgggtga cgacctcctc cacccgtcgc ccggccttgg tgcggtcgag
     4321 tggcagggcg cgcacgccca gcgtgctgct gttctcaatc agcacgcgat cgagctcggc
     4381 gcggtcggcg gccgcgcaga tgacgctgac ctgggtcgcc gggcggccgc gcttcatgat
     4441 gatcggggtc aggaagacgt cgagcgcgcc ggccgcgaag aggcgctcga tcagcggctc
     4501 gtagaactcg gggttcatgt cgtcgatatt ggtctccagc aatagctcgc tggggccgct
     4561 gtcgtcgccg ggcggggctg ctgccgggtc cagctcgccc agccagaggc gcagcgcgtt
     4621 gggccagggc agctcgcgct gcccgaatcc gtagccgata gccgaggcgt gcagcgcggg
     4681 gcgctcgaag cgggccaggc gggtcagcac cgccgcgccg gtcggggtca gcagctcggc
     4741 ctcgatctcg cgcgccaccg tgggcgcgcc gctggccgct aagagctgcg ccgtggccgg
     4801 ggcgggcacg ggcagcagac cgtgccgggt cgagacgaag ccgctgccca ggctgagcgg
     4861 gccgcagtag accgcctcga ctccgagcag ctgcagcccg gccgcggcgc cgaccacatc
     4921 gacgatcgaa tcgacggcgc ccacctcgtg gaaatgcacc gcatcggccg gcacgccgtg
     4981 gacgcgcccc tccgcctcgg ccagcgcggc gaagacagcg agcgcgtgct ggcggacggg
     5041 atcgggcagc gccgcgcgct cgatcagcgc ccggatgtcg cgccaggatc gcgcttgctg
     5101 ctctcccgcc acgcgcacgc tgacccgcgt gccgctgacg ccgtgctgtg tcactcgctc
     5161 ggccgccagc tcgtagccgc tgaggtcgag cccggccagc atgcccagca ggtcgtcgag
     5221 cgccagccca gcgtcgacca acgcgccgag gatcatgtcc ccgctggcgc cggagaaggg
     5281 atcgaaatag gcgacgggca cgacgcctcc taggctgtcg cggcctgctg ctcgtgctcg
     5341 acgcggagct ggccgcaggc ggcggcgatg tcgaccccgc gcgagtagcg caccgtggcc
     5401 gggatgcctg cctcgcgcag cagcgcggcg aagcgctcga tgcgctcgac cgaaggccgc
     5461 tcgaagggcg cggcgggtgt cgggttgagc gggatgacgt tgacgtggca gagcagcccc
     5521 ttgagcagcg cggccagctc acgcgcgatc gcatcgctgt cgttgacgtc cttgatcagg
     5581 gcgtactcga aggtgacgcg gcgcttggtg cgcgccacgt agcggcggca ggcggccatc
     5641 agctcggcca cgccgtagcg ccggttgagc ggcaccaatt ggccgcgcag ctcgtcgttg
     5701 ggcgcgtgca gcgagatggc gagcttgacc tgatagggct cctcggccag cttgtcgatg
     5761 aacgggacca gaccggaggt cgagaccgtg gtgcggcgcg agccgaagtt cagcccggcc
     5821 tcgtcgtgca ggatcgcgat catcttcagc acgttggcgt agttctggaa aggctcgccc
     5881 atgcccatca tcacgatgtt ggtcagcgtg cggccgcggg cccgcgccgc gcgggccgcc
     5941 tcgatcacct gcgctaccat ctcgccggcc gtcaggttgc gcgtcagccc catcatgccg
     6001 gtggcgcaga aggcgcagcc gaccgcgcag ccgacctgac aggagacgca gacggtggtg
     6061 cggtccgggt agaacatcag caccgtctcg atgtgctcgc cgtcgaaggt gcgcagcagc
     6121 agcttgaccg tctcgccgtc gtcggagacg gcctggcgca cgggggtgag ctgggagaag
     6181 gggaggcgct cggccagctc ggcacgcagc gtcttgggca gcaccgtcat ggtggcatag
     6241 tccggcgcga gctgttggta ggcccagtgg tagagctggc gcgcgcggta gcggggtaag
     6301 ccaagctcgc ctagccgcgc ctccagatcc ggcagcgtca agtcgtagag cgtcaccggg
     6361 cgctcggcgc ggcgacgcag cggccgggcc gcgggagcgg ttcgcggcgc gtcggttgtg
     6421 gtcagatcaa tggtcatcgt cgggttgtcc ggtgaaaagc ggcatggttc acagcaacac
     6481 gcaattgtac caaagcggca cggtcgcgaa ccgacggccc gcgtcctggc tgggagaagg
     6541 caatggccgt ggcgggtccc accccgattg gtacccaggt ccattgtcct gcccgactcc
     6601 agttcgtagg gtatgggagt gagcgcggat tcgccggttg gccgtgggac cgctcagtct
     6661 cgacagatca gcgggatcgg aggaagggtg cgcaagacgc tccgctggct cggcctcatc
     6721 acactcatgc tggtgaccct ggcgtgcagc cagcagagcg tctcctcgcc cccgccggcc
     6781 gcgcagttcg ccgcgaccac ggccatcgcc ggcgcgacac cgaccccggc gcccaccgtc
     6841 acgcccgcgc cgaccgcgac cacaccaccc ggcacgccca agccggccgc gaccgccgcg
     6901 ccgaccgtgg cgccgacccc caatcccaac gccacctggg tgccgctcaa cgcgatcgac
     6961 caggacctct cgcacggcct gctgcgggag accgatctcg acaagacctg gagcaagcag
     7021 gacgcggtcg atcacacctt cgtgatggcg ctgaaccaga tcaactgcga cggcgctcag
     7081 ctcaccgagc cggtgccgcc gcagcgcagc gccgagatcg cctaccagcg tgacgcgggg
     7141 tatctgcccg agccgctctt gatccagctc aactgggcgc tgccgagcga cgacgcggcc
     7201 gataccgaca tggatttcat caagcaggcc ctgcgctgct ccaactggga gaacgcctcg
     7261 cccggcgcgg ctgtcggcag cgtcgatatc cgctcctacg acctcgccaa gctgggggac
     7321 gacagtgtgg ccgcgcggat gaacatcagt ctcaacggca ccgccctggt gctcaccagc
     7381 gcctacgtcc gtagcggctc ggtcatcacc aatgtggtct acgtcgacac caagccggcc
     7441 gacccgaagg agttcgaggc gctggccaag aaggcgatcc agcgcgccgc ctcgctgcat
     7501 ttgtagggtg ggacggcctt cggatagaca tcgaggcgcg tgaggccgga tccttcgaca
     7561 agctcaggat gacagggtag ataacaggct gacgacgtcg tctgtcctcg tccgctaggc
     7621 cagcaacaag acacggtcat cctgagcgca gtcgaaggat ccgtcgtcca ctcgcgtcca
     7681 aacctcttcg tgggccaccc caccccgcgt tgcccacgcc cgcccggcat gctacaaagc
     7741 ccctgttggc attgaacggg aggagcgagt gcgatggcgg acgccatggt cgagcggttg
     7801 cgggccaatc tccaggcggc ggggatcgag gtcgacgagg cagacctgaa cggcatggtc
     7861 gagaagggct tcctgcgctc gatccaggcc gcggaagagg ccgtcagcac cttctccgtc
     7921 gagctggtgc ccgactacct caagccctgg ggcgatgtcg cggccgagta cagcagccct
     7981 ccgcgcgaga gcctgaccat cccgcacatc ccaccgcgcc ccggcttccc ctcgctggtc
     8041 gagctggccg agcacatcca cacgcgcgag gtctcgccgg tcgaggtgac gcggggggtg
     8101 ctcgagcgca tcgcccagcg cgacccggcg ctcaacgcct tccaggtcgt gctggcggac
     8161 gaggcgctgg cggcggcgcg cgaggctgag cgcgagatcg ccgccggcca cgtccgctcg
     8221 ccgctccacg gtgtgccgct ggccgtcaag gacctctacg ccatgcgcgg caccgtcacc
     8281 acggccggct cgcagatcct gggcgactgg cgcaccgact tcgacgcggc ggtcgtcgag
     8341 cggctgcgcg atgccggcgc ggtgatcgtc ggcaagacgc gcctgtcgga gttcgcctac
     8401 tcgcccggct ccaacaacgc gcactacggc ccgacgcgca acccctggaa cctggagcac
     8461 gataccggcg gctcctccag cggctcggcg gcggcggtgg ccgacggcct ggtctatggc
     8521 gcgctcggct ccgacaccgg ttgctcgatc cgcactcccg cgtcgctctg cggcatcgtc
     8581 ggcatcaagc cgaccttcgg tcgcgtcagc ctcttcggcg gggtgacgct ctcctggtcg
     8641 ctcgaccacg ccgggccgat gacccgcacg gtggccgacg gcgcggtcat gctcgcgatc
     8701 ctggccggct acgacgcgcg cgacatccgc acgcgcccgg tgccggtgcc cgactacgcc
     8761 gccacgctca acgagggtgt gcgcgggttg cgcgtggggg tgctgcgcga cgacggctcc
     8821 ggcgagccgc tgggtacgcc cgaggtgctg gccgcctggc gagccggggt ggcggcgctg
     8881 gacgcggcgg gcgccgagct gatcgagatc gacctgccgc agctccacga gctgcgctcg
     8941 atcaacagcg cgatcatcgc cgccgaggcc gccgccttcc accagccgtg gctccaggag
     9001 cggctcgctg actacggcga gttcccgcgc cagcggc
//
LOCUS       DASUBI010000431         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1349693, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000431 DASUBI010000000
VERSION     DASUBI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349693"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFU72_08280"
     CDS             complement(<1..371)
                     /locus_tag="VFU72_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423520.1"
                     /translation="MVENQEMLEWIDERQQRFTAVADEIWRKPQVALCETEACALQIQ
                     ELEREGFKITRNVGDLPTAFVAEWGSGRPIIGFLGEYDALPGLSQKLQATQDPEVAGG
                     PGHGCGHNLLGTSAMAAAMAV"
     gene            641..1117
                     /locus_tag="VFU72_08285"
     CDS             641..1117
                     /locus_tag="VFU72_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423521.1"
                     /translation="MSGNTGNQGTQGTQGNQNTVTNHEHHHHHFHHHYNINHGTVQGQ
                     PLSQGQGQGMQTGQRMGGPRMGQGQGQGQGFAQGMGPQSRGQGFGGYGQGPSMNMNRG
                     MGHGSGMRMQQGSPQGQAMRMQQGRQGFGNYGNRPGQGQGFGGYGFNPGQNLGTHR"
     gene            complement(1427..3934)
                     /locus_tag="VFU72_08290"
     CDS             complement(1427..3934)
                     /locus_tag="VFU72_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU5423522.1"
                     /translation="MYLIDQQNEALEYRRRYQGLIGIASKVPIRDRSMLSLVYTPGVA
                     EACREINREALASFDLTMRGNTVAIITDGSDLFGLGEGTPEMALPILEARSVVFKTFA
                     GVDAFPICLAERDPFRIVETSLALTPTFGAICLDGIAAPDSFTIADHLEKGASIPVVS
                     NQHHSSAILVLGALINALKVVDKQMDQIRVVVAGAGVSGIGIARLLTRVGVRDLVVCD
                     RAGAIYPYRPERMNWAKVYLAKETNPEGRHGSLAEMLKGADVFIGVATGDIVTPEMVQ
                     SMAPSPIVFSLALPTPEIAPGEARAAGAAVVGTARSDYPNTMDISLVFPGVFRGLLDS
                     RARDIRLRTLVYAAEALAGMVRPDELHADHILPNIFDFRVAPAIAAAVVRAACETGEA
                     ARAIDPQTVAEDTLRYVYEGKLRPRVAAKRSGTLREDAIDLRKRHNGVVEIRSKIPIK
                     DQHILNLLYLPPAALAPAVEIQENPLSVFDLTVKENLVAIVTDGSAVLGLGDIGPQAA
                     LPVMEGKAVLFQSLANVEAFPICLAERDPAKIVDIVAAISPSFGGINLEDISAPRCFE
                     IEAALKERLHLPVFHDDQHGTAVIVLAALINALKLRGTPLSDVRVVINGAGSAGVAVA
                     KLFLVVGVGDVILCDRQGIISRDRDDGGNRIKAEIARLTNKDQRSGSLADALRGADVF
                     IGVSAAGALTQDMIRTMAPSAVIFALANPEPEILPDLAREAGALVVATGRSDFPNQVN
                     NSLAFPGIFRGALDAKARDINEAMKLAAAQAIADLVDPKELSTDFIIPDALDLRVPPA
                     VAAAVAQAAVESGVAQVSIDPDEVAERCRDAVFEGQY"
     gene            4204..6153
                     /locus_tag="VFU72_08295"
     CDS             4204..6153
                     /locus_tag="VFU72_08295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5423523.1"
                     /translation="MAEPLSPEVLVYGFTTAADPQISPDGSSIIYTLGNVDKETKKGT
                     SQLWRCDLDGGDRRQLTQTGTRNSSGRWSPDGASIAFVSDRVKQAGLFVMPADQPGEA
                     RELTHHGVAITDPVWSPDGKQIAYSAEFDPENPEEKEPEEGAAPPVRVTRRIDYKQDG
                     RRYLGDKRTQVFVVDVASGERRMVTQEPVDHYFPQWSPDGKTLAVSIPNRNGMHAQLA
                     LVDVASGETQRITPEDGSVAVWSWSPDGTRLFYAGDLEQSWQSDFFVYDVAAGQSTRI
                     TDDLPVVPMGGFPGVMGPSIPVWLDEHTILFNAVRAGAGGLYTINLQDRELEKLYGDD
                     ATLSGLSIDDARRHIVQGYTSFAATGEISVYDTQTRRAQVVTDYSHATLEEHPPARWE
                     RFEVPRGKYSIEAWLMKPADFDPAKKYPVVLDIHGGPNGFYGYAFNPIQEMLATNGFL
                     LVFSNPRGSSSYGRDFTQQVIGDWGGEDFKDLMAVIDKVLEEPYADASRTGIFGYSYG
                     GYMTSWTIGQTQRFQACVCGAPCFDLESFYGTSDIGHSFGKLQFGSAPHEGKEWYAAH
                     SPSNFAHRATTPTLIIHGEADERCPIGQGEQMFVALKKAGNEVEFARYPGGAHGFVRM
                     GPPEHRVDVLTRILGWFKGHLGEPA"
     gene            6296..>6834
                     /locus_tag="VFU72_08300"
     CDS             6296..>6834
                     /locus_tag="VFU72_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423524.1"
                     /translation="MAQPMSAELLIYDLKNAADPQLSPDGTTILYTLQTLDKESKKPA
                     SQLWLRAPDGSNPRQLTRTGQRNRGGRWSPDGESIAFVSDRVGKGKAGLFVLDVARPG
                     EAREVTRHNQELNSLAWSPDGKTIAYTTTYDPENPEETERDKDAAPPVRVTRRYDYKW
                     DGRGYVGEKRGQIWTVDVAS"
BASE COUNT         1154 a         2346 c         2335 g          999 t
ORIGIN      
        1 ttgaccgcca tcgccgccgc catggcgctg gtgcccagca ggttgtggcc gcagccgtgg
       61 cccgggccgc ccgcgacctc gggatcctgc gtcgcctgga gcttctgcga gaggccgggc
      121 agcgcgtcgt actcgccgag aaagccgatg atcggccgtc cgctgcccca ttcggccacg
      181 aaggcggtcg gcagatcgcc cacgttgcgg gtgatcttga agccctcgcg ctccagctcc
      241 tgaatctgca gcgcgcaggc ctcggtctcg cacagcgcca cctgcggctt gcgccagatc
      301 tcatccgcca ccgcggtgaa gcgctgctgg cgctcatcga tccactcgag catctcctgg
      361 ttttcgacca cgattgccct cctcgtttca atcccccgac atcgcgtcgg ccagtgagca
      421 gtacggcgct accataccat cacctcctga cgagggatag cccacgaatg gccacccgag
      481 gttgccaacg gtcgcacaga cagtttgctt ccggcatggg ttctgcaggg gggctgacca
      541 cgggcgaggg ctgccggcca gtcgatgggc gagtctacgg ctgccggcgc gcccggacgc
      601 acgcggcgag gctcgcatcg tactactcgg aggaagcaac atgtcgggta acaccgggaa
      661 ccaggggacc caggggacgc aagggaacca gaacacggtc accaaccacg agcatcacca
      721 tcaccatttc caccaccact acaacatcaa ccacggcacc gtgcagggcc agccactgag
      781 ccagggccag ggtcagggca tgcagaccgg gcagcgcatg ggcggcccga ggatgggcca
      841 gggccagggc cagggtcagg gcttcgcgca gggcatgggg ccgcagagcc gtgggcaggg
      901 cttcggcggc tacggccaag gcccgagcat gaacatgaac cgcggcatgg gccacgggtc
      961 gggtatgcgc atgcagcagg gttcgccaca gggccaggcc atgcgcatgc agcagggccg
     1021 gcagggtttc ggcaactatg gcaaccgccc cggccagggt caggggttcg gcggctacgg
     1081 cttcaatccg ggccagaatc taggaaccca caggtaggac tcgtcacccg tcgttggacc
     1141 gggtaccggc ggccatgccg cacctcgatc cagcggcccc gtcgccgcag ccgcgtgcgt
     1201 cgcgcctcgc cccagcggtg aggcgcgact cgtgtcgagc tgaccgagct gacattgatg
     1261 gtggggtacc ggggaaaacg gatcacccac cgcggtcggg atgacaatgg aaaggggatg
     1321 gggatggcct accgggcgcg gctcatcgtc gagcgctcct cattaccccg gtcattccga
     1381 ccctgtcgag agttccgtcc tcgatcgcta caaggccccg tcgttctcag tactgcccct
     1441 cgaagacggc gtcgcggcag cgctcggcga cctcgtcggg gtcgatgctg acctgcgcca
     1501 ccccgctctc gaccgccgcc tgcgccacgg ccgccgccac cgcgggcggc acgcgcaggt
     1561 cgagcgcgtc ggggatgatg aagtccgtgc tcagctcctt gggatcgacc agatcggcga
     1621 tcgcctgggc ggccgccagc ttcatcgcct cgttgatgtc acgcgccttg gcgtcgagcg
     1681 cgccgcggaa gatgccgggg aaggccagcg agttgttcac ctggttgggg aaatccgagc
     1741 gcccggtcgc gacgaccagc gccccggcct cgcgcgccag gtcgggcaga atctccggct
     1801 cggggttggc cagcgcgaag atgaccgcgc tgggcgccat ggtgcggatc atgtcctgcg
     1861 tcagcgcgcc ggccgccgag acgccgatga agacatcggc gccgcggagc gcgtcggcca
     1921 gcgagccgct gcgctggtcc ttgttggtga ggcgggcgat ctccgccttg atgcggttgc
     1981 cgccgtcgtc gcggtcgcgc gagatgatcc cctggcggtc gcagaggatg acgtcgccga
     2041 cgccgacgac caggaagagc ttggcgaccg ccacgccggc cgagccggcg ccgttgatca
     2101 ccacccgcac gtcgctgagc ggcgtaccgc ggagcttgag cgcattgatc agggcggcga
     2161 gcacgatcac ggcggtgccg tgctggtcat cgtgaaagac gggcaggtgc agccgctcct
     2221 tgagcgcggc ctcgatctcg aagcagcgcg gcgccgagat gtcctccagg ttgatgccgc
     2281 cgaacgaggg tgagatggcg gccacgatat cgacgatctt ggccggatcg cgttcggcca
     2341 ggcagatcgg gaaggcctcg acgttggcca gcgactggaa gaggaccgcc ttgccctcca
     2401 tcaccggcaa ggccgcctgc gggccaatgt cgcccagccc cagcaccgcg ctgccgtcgg
     2461 tgacgatcgc taccaggttc tccttgaccg tgaggtcgaa gacggagagc gggttctcct
     2521 ggatctcgac ggcgggcgcc agggcggccg gcggcaggta gagcaggttg aggatgtgct
     2581 ggtccttgat cgggatcttg ctgcggatct cgaccacgcc gttgtgccgc ttgcgcaggt
     2641 cgatcgcgtc ctcgcgcagc gtgcccgagc gcttggcggc cacccgtggc cgcagcttgc
     2701 cctcgtagac atagcgcagc gtgtcctcgg ccactgtctg cgggtcgatg gcgcgtgccg
     2761 cctcgccggt ctcgcaggcc gcgcgtacca ccgccgcggc gatcgccgga gcgacgcgga
     2821 agtcgaagat attgggcagg atgtggtcgg cgtgcaactc gtccggccgc accatgccgg
     2881 ccagcgcctc ggcggcgtag accagcgtgc gcaggcggat gtcgcgcgcg cgcgagtcga
     2941 gcagcccgcg gaagacgccg gggaagacga gcgagatgtc catcgtgttg gggtagtcgg
     3001 agcgggccgt gccgaccacg gcggcaccgg ccgcacgcgc ctcgccgggc gcgatctccg
     3061 gtgtcggcag cgccagcgag aagacgatcg ggctgggagc catcgactgg accatctccg
     3121 gggtgacgat gtcgccggtc gccacgccga tgaagacgtc ggcgcccttg agcatctcgg
     3181 ccaggctccc gtggcgtccc tccggattgg tctccttggc cagatagact ttggcccagt
     3241 tcatccgctc ggggcggtac gggtagatcg ccccggcgcg gtcgcagacg accaggtcgc
     3301 gcacgccgac acgggtcagc agacgggcga tgccgatgcc ggagacgccc gcccccgcca
     3361 ccaccacccg gatctgatcc atctgcttat cgacgacctt gagcgcgttg atcagggcgc
     3421 cgagcaccag gatggccgag ctgtggtgct ggttgctgac gaccgggatg ctggcgccct
     3481 tctccagatg gtcggcgatg gtgaagctgt cgggggcggc gatgccgtcg aggcagatcg
     3541 cgccgaaggt gggcgtcagg gcgagcgagg tctcgacgat gcgaaagggg tcgcgctcgg
     3601 ccaggcagat cgggaaggca tcgacgcccg cgaaggtctt gaagaccacg ctgcgcgcct
     3661 ccaggatcgg cagggccatc tcgggcgtgc cctcgcccag gccgaagagg tcggagccat
     3721 cagtgatgat ggctacggtg ttgccgcgca tggtgaggtc gaacgacgcc agggcttcac
     3781 ggttgatctc gcggcaggcc tccgcgacgc ccggtgtgta gaccaggctg agcatcgagc
     3841 ggtcccggat cggcaccttg ctcgcgatcc caatcaggcc ctgatagcgt ctccgatatt
     3901 ccagcgcctc gttctgctga tcaatgagat acacagagaa atccaccacc agttcaggat
     3961 aaaacagtcg gacagcgcca ggccgtacct tcgcattgta cacccgcccg cggtctcgct
     4021 ggcagccgaa gcgaaccgat tctgttccga tcatctccgc gggtatcctg ttgccagcac
     4081 gccggtcccg gcgtagcatc cagatcggag ggaggacgcg acatcgcgcc cgcggtcgcg
     4141 gtgtccacgc tgcgtctgga gctcctgcgg tcagtcgaac aagggataca gagggaggaa
     4201 gcggtggcgg aaccattgtc acccgaggtg ctggtctacg ggtttactac tgcggctgac
     4261 ccgcagatct cgcccgacgg cagctcgatc atctacaccc tgggcaacgt cgataaggag
     4321 accaagaagg ggacgagcca gctctggcgc tgcgacctcg acggcggcga ccggcgccag
     4381 ctcacccaaa ccggcacgcg caacagcagc gggcgttggt cgcccgacgg cgcgtcgatc
     4441 gccttcgtct ccgatcgggt caagcaggcg ggcctcttcg tgatgccggc cgaccagccg
     4501 ggcgaggcgc gcgagctgac ccaccacggc gtcgccatca ccgacccggt gtggtcgccc
     4561 gacggcaagc agatcgccta cagcgcggag ttcgaccccg agaatccgga ggagaaggag
     4621 ccggaggagg gcgccgcccc gccggtacgc gtcacccgcc gcatcgacta caagcaggac
     4681 ggccgccgct acctgggcga caagcgcacc caggtcttcg tggtcgacgt cgcctccggc
     4741 gagcgccgca tggtgaccca ggagccggtc gatcactact tcccgcagtg gtcacccgac
     4801 ggcaagacgc tggccgtctc gatccccaac cgcaacggga tgcacgcgca actggcgctg
     4861 gtcgatgtcg ccagcggcga aacgcagcgc atcacgccgg aggacggctc ggtcgcggtc
     4921 tggtcgtggt cgccggacgg cacgcgcctc ttctacgccg gcgatctgga gcagagctgg
     4981 cagagcgact tcttcgtcta cgacgtcgcc gccggccagt cgacgcgcat caccgacgac
     5041 ctgccggtgg tgccgatggg cggcttcccc ggcgtgatgg gaccgtcgat cccggtctgg
     5101 ctcgacgagc acaccatcct cttcaacgcc gtgcgcgccg gagccggcgg cctctacacc
     5161 atcaacctgc aagaccgcga gctggagaag ctctacggcg acgacgccac cctctccggc
     5221 ctgagcatcg acgacgcgcg gcggcacatc gtccagggct acaccagctt cgcggccacc
     5281 ggcgagatct cggtctacga cacccagacc cgccgcgcgc aggtggtgac cgactacagc
     5341 cacgcgacgc ttgaggagca cccgccggcg cgctgggagc gcttcgaggt gccgcgcggc
     5401 aagtacagca tcgaggcctg gctgatgaag ccggccgact tcgacccggc caagaagtat
     5461 ccggtggtcc tcgacatcca cggtggcccc aacggcttct acggctacgc cttcaacccg
     5521 atccaggaga tgctggcgac caacggcttc ctgctggtct tcagcaaccc gcgcggctcg
     5581 tcgtcctacg ggcgagactt cacccagcag gtgatcggcg actggggcgg cgaggacttc
     5641 aaggacctga tggcggtcat cgacaaggtg ctggaggagc cctacgccga cgccagccgc
     5701 accggcatct tcggctacag ctacggcggc tacatgacct cgtggacgat cggccagacc
     5761 cagcgcttcc aggcctgcgt ctgcggcgcg ccctgcttcg acctggagtc cttctacggc
     5821 accagcgaca tcggtcactc cttcggcaag ctccagttcg gctcggcgcc gcacgagggc
     5881 aaggagtggt acgcggcgca ttcgccctcg aacttcgccc accgcgccac caccccgacc
     5941 ctgatcatcc acggcgaggc cgacgagcgc tgcccgatcg gccaggggga gcagatgttc
     6001 gtggcgctga agaaggccgg caacgaggtc gagttcgccc gctaccccgg cggcgcgcac
     6061 ggcttcgtgc gcatgggccc ccccgagcat cgcgtggacg tgctgacgcg catcctcggc
     6121 tggttcaagg gccacctggg cgagccggcg taggccctca cccccaaccc gggggccctc
     6181 accccaaccc ctctcccctg cgggggcgag gggccaggat gaagttgtag gggcgggttt
     6241 gaaacccgcc ctcgaacccg ccggcgacga cgatgatagg agagggagac aacgcatggc
     6301 gcagccgatg agcgcggagc tgctgatcta cgacctcaag aacgcggccg atccgcagct
     6361 ctcgccggac ggcacgacga ttctctacac gctacagaca ctcgacaagg agtccaagaa
     6421 gccggccagc cagctctggc tccgcgcccc tgacggctcc aatccgcggc agctcacgcg
     6481 gaccggccag cgaaatcgcg gtgggcgctg gtcgcccgat ggggaatcca tcgccttcgt
     6541 ctccgaccgg gtcggcaagg gcaaggccgg cctgttcgtg ctcgacgtgg cgcggcctgg
     6601 tgaagcgcgc gaggtcacca ggcataatca agagctgaac tcgctggcct ggtcgccgga
     6661 cggcaagacg atcgcctaca cgacgacgta cgaccctgag aaccccgaag agacggagcg
     6721 cgacaaggac gcggctcccc cggtgcgcgt cacccgccgc tacgactaca agtgggacgg
     6781 ccggggctac gtcggcgaga agcgcggcca gatctggacg gtcgatgtgg cctc
//
LOCUS       DASUBI010000432         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_603467, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000432 DASUBI010000000
VERSION     DASUBI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603467"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFU72_08305"
     CDS             complement(<1..1297)
                     /locus_tag="VFU72_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423525.1"
                     /translation="MDATIDNATSPPDRRPWYAVGFADVVLVFFTLCTLQTAGTRMMD
                     DPGLGWNLRVADLLRERGGFLYSEEFCFPTEGRPWVTQAWLGDILLRLSYGWGGLNGL
                     AVFSALCIALTLRLLYVRMTRDGIHWLAAALWTFLAALGTSPSWVARPNLFTFPLLVL
                     VADLCDRFHRGTISPRATLWLLPVFALWPNLHGGFLAGILVLGVTYLVECGFAVAAPD
                     ADARRAARARLRWWTILGVGLFVATLLNPYGIGLHFWNLRMLRDPFIQTQTTAEWLPP
                     NFNDKGWFRIEALVLLFPLLGVLSRRRVSAVALALAVVFLHFGLTSARYTPLWVVLAV
                     PSLALLAAGVPRFEALSADVSARLSPDVRAGLARRPGPSPCGASWAFAVLLLAASPWL
                     GPLARHNQELMPSRALDALLADYRGERVFHSANWGGYLTW"
     gene            complement(1331..1873)
                     /locus_tag="VFU72_08310"
     CDS             complement(1331..1873)
                     /locus_tag="VFU72_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5423526.1"
                     /translation="MYSPPYQGGARGGRTSCQHESDAIPAFGAAAYEAVLALDVIEHL
                     DDDRAAVAGLARLVAPGGCLVVSVPALPELYTEFDAVQDHRRRYVPETLRAAFDGSGL
                     EVERLFWWGSWLVPLLKRQRAQAATAPTAARRYNEYLRLPPWPATVALRALFAWEHGR
                     SLAGRSTIGTSLFAVGRRRG"
BASE COUNT          362 a          747 c          705 g          235 t
ORIGIN      
        1 gccaggtgag gtagcctccc cagttggcgc tgtggaacac ccgctcgccc cggtagtccg
       61 cgagcaaggc atcgagcgcc cgcgagggca tcagctcctg gttgtgccgc gccagtggcc
      121 cgagccacgg cgacgctgcc agcagcaaca cagcgaacgc ccagctcgcc ccgcacggcg
      181 atgggcctgg gcgacgcgct agccctgccc gaacgtcagg cgagagccgt gccgagacgt
      241 cggcgctcag cgcttcgaac cgcggcaccc cggccgccag caaggccagc gatggcaccg
      301 cgagcacgac ccacagcggc gtgtaccgcg ccgaggtcag cccgaagtgc aggaagacga
      361 ccgccagtgc caacgccacg gcgctcacgc gccgtcggct caagacgccc agcaagggaa
      421 acagcagcac cagcgcctcg atgcgaaacc accccttgtc gttgaagttc ggtggcagcc
      481 attcggcggt cgtctgggtc tggatgaacg ggtcgcgcag catccgcagg ttccagaaat
      541 gcagcccgat gccgtaggga ttcagcagcg tcgccacgaa caggcccacg ccgaggatcg
      601 tccaccaccg gagtcgcgct cgcgccgcgc ggcgggcgtc ggcgtcgggt gctgcgacgg
      661 cgaacccgca ctccaccagg tacgtcaccc ccagcactag gattcccgcc aggaatcccc
      721 cgtgaaggtt aggccagagc gcaaaaacgg gcagtagcca cagcgtcgcg cgcggcgaga
      781 tggtgccacg gtggaagcgg tcgcacaggt cggccaccag cacgagcagc ggaaacgtga
      841 acaggtttgg ccgcgccacc cacgacggcg acgtgccgag cgcggcgagg aacgtccaga
      901 gcgcggcggc gagccagtgg atgccgtcgc gggtcatgcg cacgtagagc agccggagcg
      961 tcagcgcgat gcaaagcgcc gagaacacgg cgagcccgtt cagccccccc cagccgtacg
     1021 acaggcgcag caggatgtcg cccagccagg cctgcgtgac ccaggggcgg ccctcggtcg
     1081 ggaagcagaa ctcttcgctg taaaggaacc cgccgcgctc tcgcaacagg tcggcgacgc
     1141 gcaggttcca gcccagcccc gggtcgtcca tcatccgcgt accggccgtc tgcagggtgc
     1201 agagcgtgaa gaacacgagc acgacgtcgg cgaagcccac ggcgtaccag ggccggcggt
     1261 ccggtgggct cgtagcgttg tcgatcgtcg cgtccatggc gtgcgttcca cccctggttt
     1321 ctcgaaaggc tcaaccgcgc cgacggccga cggcgaacag cgacgtaccg atcgtcgacc
     1381 ggcccgccag gctccggccg tgctcccacg cgaacaacgc ccgcagcgcg acggtcgcgg
     1441 gccagggcgg caggcgcagg tattcgttgt agcgccgggc cgcggtggga gctgtcgcgg
     1501 cttgcgcccg ctggcgcttc agcagcggca cgagccacga gccccaccag aacagccgct
     1561 ccacctccag gccgctgccg tcgaacgccg cgcggagcgt ttcgggcacg taccgtcggc
     1621 ggtggtcctg cacggcatcg aactcggtgt acagctcggg caaggccggc acgctcacga
     1681 cgaggcaacc ccccggcgcc acgagccgcg ccaggccggc cacggccgcg cggtcgtcgt
     1741 cgaggtgctc gatcacgtcg agcgcgagca cggcctcgta ggcggcagcg ccgaacgccg
     1801 gaatcgcatc ggattcgtgc tggcaggacg tgcgaccccc cctagccccc ccttggtaag
     1861 ggggggagta aatgcgcccg cacccaggct ccccagtcgc gtcaccgagc gcggaagctt
     1921 cgcccctcga agcacaagct cccccccttg aaaagggggg gctagggggg gtctgcccgc
     1981 cgcgcccgag gccaggatcc ccagtcgcgc cgccgagcgc ggaagctccc ccccttgcaa
     2041 aggggggat
//
LOCUS       DASUBI010000433         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_154027, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000433 DASUBI010000000
VERSION     DASUBI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154027"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1099)
                     /gene="gap"
                     /locus_tag="VFU72_08315"
     CDS             complement(83..1099)
                     /gene="gap"
                     /locus_tag="VFU72_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5423527.1"
                     /translation="MTVRVGINGFGRIGRQVFKAMFGEYDEDLEIVAANDLMSPQTAA
                     HLLKYDSTYGIYDDEVEVNDSSITVNGQEIKLLSERDPGKLPWKDLGVELVIESTGFF
                     TDAAKARAHIEAGAKKVIITAPAKNEDITIVLGVNEDAYDPAKHTIVSNASCTTNCLA
                     PVAKVVFDHFGIKRALMTTIHSYTGDQVLLDGPHRDLRRARAAGLNIVPTTTGAARAV
                     ALVIPELKGKFDGFALRVPTPTVSLIDFTAELEKPANADAINAVFKEVAEDQLAGILD
                     YTEEPLVSMDFKGDSHSAIVDGLSTMVLEDGMAKIVAWYDNEWGYSCRVADLAALMAE
                     QGIS"
     gene            complement(1096..>2131)
                     /locus_tag="VFU72_08320"
     CDS             complement(1096..>2131)
                     /locus_tag="VFU72_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEU5423528.1"
                     /translation="GGTGLSTLLRGLKLHNIDITAIVTVGDDGGSSGRLRTEFNIPPP
                     GDIRNCLVALADNESVMSELFQYRFEEGSSLDGHSFGNLFITALTRVSGSFEQAVVES
                     SRVLAVRGKVLPSTLEDIVVCGEMADGRVIRGESNITNERGTIKRIYLDPSEPQAYDP
                     AIIALLSADVIVLGPGSLYTSVLPNLLVPGIAHAIRCSPATKVYVCNVATQRGETDNF
                     GAIDHVLALQQHVSGGIIDYALVNSNIDPVIARIKPEWMVNAVGLAGLEGLHGVRVLL
                     RDVISAEKPLRHDPAKLASELLELARQARTARDGSGVFPDTGAPEVLRQPVTAGLPLA
                     RQPQSKESLR"
BASE COUNT          357 a          713 c          664 g          397 t
ORIGIN      
        1 agcaagcatg cccgttcccg ttcacccgca agcggccggc gcaccgttcc ggcgcgtgcc
       61 gattcgtgcc gcaaccgtcg cgctagctga tcccctgctc ggccatcagc gcggcgagat
      121 cggccacgcg acaggagtag ccccactcgt tgtcgtacca ggccacgatc ttggccatgc
      181 cgtcttcgag caccatggtc gagaggccgt ccacgatggc cgagtgcgag tcacccttaa
      241 agtccatcga gaccagcggt tcctcggtgt aatcgaggat accggccaac tgatcttcgg
      301 cgacctcttt gaagaccgcg ttgatcgcgt cggcgttggc cggcttctcc agctcggcgg
      361 tgaagtcgat cagtgagacg gtcggcgtcg gcacgcgcaa ggcaaagccg tcgaacttcc
      421 ccttcagctc agggatcacc agggctaccg cgcgggccgc cccggtcgtc gtcgggacga
      481 tgttcaaccc ggcggcccgg gcccggcgca gatcccggtg cggcccatcg agcagcacct
      541 gatcgcccgt gtaggagtga atcgtcgtca tgagcgcgcg cttgatgccg aaatggtcaa
      601 aaacgacctt cgccaccggg gccaggcagt tggtcgtgca ggaggcgttg gagacgatcg
      661 tgtgcttcgc cggatcgtag gcgtcctcgt tgacccccag cacgatggtg atgtcctcgt
      721 tcttcgccgg ggcggtgatg atgaccttct tcgctccagc ctcgatgtgc gcccgcgcct
      781 tggccgcgtc ggtaaagaag ccggtcgatt cgatcaccag ttcgaccccg agatccttcc
      841 acgggagctt gccgggatcg cgctcggaca acagcttgat ctcttggcca ttgacggtga
      901 tcgacgagtc gttcacttcg acctcgtcgt cgtagatacc gtaagtcgag tcgtacttga
      961 gcagatgcgc cgccgtctgc gggctcatca ggtcgttggc cgccacgatc tccaggtcct
     1021 cgtcgtactc gccgaacatc gccttgaaga cctggcgtcc gatacgacca aagccattga
     1081 ttccaacccg taccgtcatc tgaggctctc cttactttgc ggctgccgtg ccagcggcag
     1141 ccccgcggtg acgggctgcc ggagcacctc tggcgcgccc gtgtcgggaa acactccgga
     1201 gccgtcccgc gccgtgcgcg cctgacgtgc cagctccagc aattcactgg ccagcttcgc
     1261 cggatcgtgg cgcagcggtt tttccgcgct gatcacatca cgcagcagga cgcgcacacc
     1321 gtgcaggccc tccagacctg ccagccccac cgcgttgacc atccactcgg gtttgatgcg
     1381 cgcgatcact ggatcgatgt tgctgttgac cagggcgtag tcgatgatgc cgccgctcac
     1441 gtgttgttgc agcgccagca cgtggtcgat cgcgccgaag ttgtcagtct cgccgcgctg
     1501 tgtggcgacg ttgcagacgt acaccttggt cgccggggag cagcgaatag cgtgcgcgat
     1561 ccccggtacg agcaaattgg gcagcaccga cgtgtagaga cttcccggcc ccagcacgat
     1621 gacatcggcc gagagcagcg cgatgatcgc cgggtcataa gcttgcggtt ccgaggggtc
     1681 gagatagatg cgcttgatcg tgccccgctc gttggtgatg ttcgactcac cccggatcac
     1741 gcgcccgtcc gccatctcac cgcagaccac gatgtcttcc agcgtcgacg gcagcacttt
     1801 gccgcgcacc gccagcacgc gcgaggactc gaccacggct tgctcgaaac tgccggagac
     1861 gcgcgtcagc gccgtgatga agaggttgcc gaagctgtga ccgtccagcg acgagccttc
     1921 ctcgaagcgg tactgaaaca gctcgctcat caccgactca ttgtcggcca gcgccaccag
     1981 gcagttgcgg atatcgcccg gcggcgggat gttgaactcg gtgcgcagcc gtcctgagct
     2041 gccgccgtca tcgcccaccg tcacgatggc ggtaatgtcg atgttgtgca gcttgaggcc
     2101 gcgcagcagc gtcgagaggc cggtgccgcc g
//
LOCUS       DASUBI010000434         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_314310, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000434 DASUBI010000000
VERSION     DASUBI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314310"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VFU72_08325"
     CDS             <1..1628
                     /locus_tag="VFU72_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5423529.1"
                     /translation="EAWGVASFYALLALEPHPPLVAHVCDDIACRMAGGLELCAALEE
                     RFGPEGAPAGDGRVTWHRSPCLGLCERAPAVLLQQAGEQPRELAVAPIDAPRLLDLLS
                     GGLDGQAGESGGPRSVPQAEAPHAHRLRLLRRVGHVDPESLDAYRASGGYEGLRQALA
                     LGPEGVIREVTDARLVGRGGAAFPTGRKWNDVARAPGRPHYLICNADESEPGTFKDRV
                     LMEEDPFALVEAMTIAGFATNCAQGFIYIRNEYPLAAERLQHAIDSARRRGLLGDDVM
                     GAGVRFDIEIRRGAGAYICGEETALFNSIEGLRGEPRNKPPYPTQAGLFRKPTLVNNV
                     ETLMNVPDIMRLGGPAFAATGTEGSTGTKLFCVCGRVERPGVYEVPFGASLRQLLELA
                     GGVAGGRALRAVLLGGAAGSFVTPDELDVPLTFEGTRAIGAALGSGVIMAFDDTVDLT
                     DTLLRIAAFFRDESCGQCVPCRVGTVRQEEALRRLAAGKPLGSAAGELALLDDLAQVM
                     RDSSICGLGQTAPNAILSAIQKLRLFEDGKVSS"
     gene            1625..2665
                     /locus_tag="VFU72_08330"
     CDS             1625..2665
                     /locus_tag="VFU72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5423530.1"
                     /translation="MTALARERAVRTVTLTIDGQAVTVPEGATILEACRALSIDTPTL
                     CYLETLTPVNVCRACVVELEGSRALVPSCSRPAEPGMVVHTDSERVRRNRRLVLELLA
                     SSVDLSWSGDEALGYLERYDAQPGRFGPAAAPARGDERDRALAGHHQPTPDGQAATVA
                     QPIKIDNELYVRDYSRCILCYRCVEACGVDAQNTFAISAAGRGFEARISTELNLPLGD
                     SACVYCGNCIGVCPTGALMFKSEYDMRQAGTWDESRQTVTDTICPYCGVGCTLELHVQ
                     DNTIVKVTSPMDHDVTSGHLCIKGRFGFQFVQNRADGKGSGNGGGRSSGNGKVRPGQA
                     LERNGEGDGGAG"
     gene            2817..>3030
                     /locus_tag="VFU72_08335"
     CDS             2817..>3030
                     /locus_tag="VFU72_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423531.1"
                     /translation="MRTPGDDFALAAGFLHGEGIIAGREDVLDISYCLDEIEPQEQRL
                     NIVTVRLRPELAFELDRLQRHFYTTSS"
BASE COUNT          434 a         1078 c         1093 g          425 t
ORIGIN      
        1 ccgaggcctg gggcgtggcc agcttctacg ccctgctggc gctggagccg cacccgccgc
       61 tggtggcgca cgtctgcgac gacatcgcct gccgtatggc gggcgggctg gagctatgcg
      121 ccgcgctgga ggagcgcttc ggcccggagg gcgcgcccgc cggggatggc cgcgtcacct
      181 ggcaccgcag cccctgcctc ggcctctgcg agcgcgcccc agcggtgctc ctgcagcagg
      241 cgggcgagca gccgcgcgag ctggcggtcg cgccgatcga cgcgccgcgc ctgctcgatc
      301 tcctgagcgg tgggctggac ggacaggccg gcgagagcgg cggcccgcgc tcggtgccgc
      361 aggcggaggc gccgcatgcc caccggctgc ggctgctgcg ccgggtcggg cacgtcgatc
      421 ccgagagcct ggatgcctac cgcgccagcg gcggctacga gggcctgcgc caggcgctgg
      481 cgctgggtcc ggaaggcgtg atccgcgagg tgaccgacgc gcgcctggtc ggacgcggcg
      541 gcgccgcctt ccccaccggg cgcaagtgga acgacgtggc ccgcgcgccg ggccgtcccc
      601 attacctgat ctgcaacgcc gacgagtcgg agccgggcac cttcaaggac cgggtgctga
      661 tggaggagga tcccttcgcc ctggtcgagg ccatgacgat cgcgggattt gccaccaact
      721 gcgcgcaggg cttcatctac atccgcaacg agtacccgct ggcggccgaa cggctgcagc
      781 acgccatcga cagcgccagg cgccgcggcc tgctcggcga cgacgtcatg ggcgcgggcg
      841 tgcgcttcga catcgagatc cggcgcgggg ccggcgccta catctgcggt gaggagacgg
      901 cgctcttcaa ctcgatcgag gggctgcgcg gcgagccgcg caacaagccc ccctacccga
      961 cccaggctgg gctgttccgc aagccgaccc tggtcaacaa cgtcgagaca ctgatgaacg
     1021 tgcccgacat catgcgcttg ggcgggccgg ccttcgccgc caccggcacc gagggctcga
     1081 ccggcaccaa gctcttctgc gtctgcgggc gtgtggagcg gcccggcgtc tacgaggtgc
     1141 ccttcggcgc cagcctgcgc cagttgctcg agttggccgg cggggtagcc ggcgggcgcg
     1201 cgctgcgcgc ggtgctgctg ggcggcgcgg cgggcagctt cgtcacgccg gacgagctgg
     1261 acgtgccgct gaccttcgag ggcacgcgcg ccatcggcgc cgcgctcggc tcgggtgtga
     1321 tcatggcctt cgacgacacc gtcgacctga ccgacacgtt gctgcgcatc gccgccttct
     1381 tccgcgacga gtcgtgcggc cagtgcgtgc cctgccgcgt cggcacggtg cgccaggagg
     1441 aggcgctgcg ccgcctggcc gccggcaagc cgctcggctc ggcggccggc gagctggcgc
     1501 tcctagacga cctggcccag gtgatgcgcg actcctcgat ctgcggcctg ggtcaaaccg
     1561 cgcccaacgc gatcctctcg gccatccaga aactgcggct cttcgaagat gggaaggtct
     1621 cctcatgaca gcgctggcac gcgagcgcgc cgtgcgcacg gttacgctca cgatcgacgg
     1681 gcaagcggtc acggtgccgg agggcgcgac catcctggaa gcctgccgcg cgctgagcat
     1741 cgatactccg accctctgct acctggagac actgaccccg gtcaacgtct gccgcgcctg
     1801 tgtggtcgag ctggagggct cgcgcgcgct ggtgccgtcc tgctcgcgcc cggccgagcc
     1861 gggcatggtg gtgcacaccg acagcgagcg ggtgcgccgg aaccgccgcc tggtgctgga
     1921 gctgctggcc tcctcggtcg atctctcctg gtccggcgat gaggcgctgg gctacctgga
     1981 gcgctacgac gcccagcctg ggcgcttcgg ccccgccgcc gctccggcca ggggcgacga
     2041 gcgcgaccgc gcgcttgccg ggcaccacca gccgacgccg gacggccagg ccgccaccgt
     2101 ggcccagccg atcaagatcg acaacgagct gtatgtgcgc gactactcgc gctgcatcct
     2161 ctgctaccgg tgcgtcgagg cctgcggcgt ggacgcgcag aacacgttcg ctatctcggc
     2221 ggcggggcgg ggtttcgaag cgcgcatctc gaccgagctg aacctgccgc tgggcgactc
     2281 cgcctgcgtc tactgcggca actgtatcgg cgtctgcccg accggcgcgc tgatgttcaa
     2341 gagcgagtac gacatgcgcc aggcggggac ctgggacgag tcacggcaaa ccgtgaccga
     2401 caccatctgc ccctactgcg gcgtcggctg cacgctggag ctgcacgtgc aggacaacac
     2461 gatcgtcaaa gtcacctcgc cgatggacca cgacgtgacc tccggccacc tctgcatcaa
     2521 gggtcgcttc ggcttccagt tcgtgcagaa ccgcgcggac ggcaagggca gcgggaatgg
     2581 cggcggccgg agtagcggca acggcaaggt gcggcccggg caggcgctgg agaggaacgg
     2641 ggagggcgac ggtggcgctg gctgaaccgc ctgcccagac cgcgcacccc atcgtgcgcc
     2701 tgcacgacgg ccagtggcac gacgcgggcg atgtgctggc agtcgaggag ccgttgtaga
     2761 tccgcctgct ctgggaggaa gacggtcagg agcggcgcga gagcatcgcg atcacgatgc
     2821 gcacccccgg cgacgacttc gcactggccg ccggtttcct gcacggcgaa gggatcatcg
     2881 ccggccgcga ggatgtgctc gacatctcct actgtctgga tgagatcgag ccgcaggagc
     2941 agcggctgaa catcgtcacg gtgcggctgc gccccgagct agcattcgag ctcgaccggc
     3001 tgcagcgcca cttctacacc acctccagct
//
LOCUS       DASUBI010000435         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_873511, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000435 DASUBI010000000
VERSION     DASUBI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873511"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFU72_08340"
     CDS             complement(<1..755)
                     /locus_tag="VFU72_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5423532.1"
                     /translation="MTDTANRTVESLLSSRAQWLGGGRLANPRASASLISFVAGFPDP
                     GSLPKRDITEATRVALERDGEWALQYGAGRGYPELVQELLTKLKRDQGIEASEENVLI
                     TAGAGQALGLVCEALCDPGDVILSEEPTWSGAVHAFRRVAAEVVPVPLDAEGTDTAAL
                     EATLTQLAAEGRAAKLLYMIPTFQNPTGVTTTLARRRRIIELSRQYGFFIVEDDAYFD
                     LRFEGTRVPTLYSLAPDRAAYLGTFSKIMAAGMR"
     gene            951..1985
                     /locus_tag="VFU72_08345"
     CDS             951..1985
                     /locus_tag="VFU72_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5423533.1"
                     /translation="MGETREIFDMTIIGGGPIGLFAAFYAGMRGLRTKIIDSLEELGG
                     QLIAVYPEKYIYDVAGIPKILAKEYVTGAVEQGLSTGAVAVLGEEVRLLERLEDEQLL
                     HLVTSKGDHWSRTVLIAAGVGAFEPKRLEAPGVNELEGRGVHYFVRDLEQFRDKDVVI
                     VGGGDSAVDWALAVVPLARRLTVVHRSKFRAHERSIAELESSAAQLYFPFYEVKEARG
                     NGNLEEIAFQHTRTGEEHRVPAQALIVAIGFGGNLGPLKEWGFEIERNMIVVDPLTME
                     TGMAGVFAAGDIITHPVKFKLIATGAAEAVIAVNHAVTYIDPKARLDAGHSTNLMERR
                     ERQREREHES"
     gene            2311..3243
                     /locus_tag="VFU72_08350"
     CDS             2311..3243
                     /locus_tag="VFU72_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423534.1"
                     /translation="MGRFIARRLIWLVPTILAMSLITFALMHATPGSPLQPMAANANP
                     LPPGAQENLKHAWGLDKPLWQQYVTFLWKGLHLDFGTSYVFRTRAVSDILRQTFPISL
                     ELGLMALGLAIIIGIPLGILAAVNQNGPIDYLSSFMATLGVAVPNFVLGLFMIVLFVL
                     ILHILPDTGGWNGPVDWIMPALALGLGPLGILARYTRSSMVEVLRQDYIRTAQAKGLA
                     PSRVVIAHAVKNGLIPPLTILGPMFAAIGTGSFFVETIFRVPGMGQFFVTSMINRDYP
                     MIMAVILLYGVFLAFMNLVVDVCYGVVDPRIRLS"
     gene            3253..4203
                     /locus_tag="VFU72_08355"
     CDS             3253..4203
                     /locus_tag="VFU72_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423535.1"
                     /translation="MDALDKPQAQDLSQLEAKSAEVASGASALSAAARQHRTLWRDAF
                     HRLVQNKLAVAGGIVILIIVAMALFASLVAPEPYATPNFAAINQFPSSAHWLGTDELG
                     RDFLSRLIYGAQVSMLVGLGAQFIVLLIGVPIGAIAGYAGGKVDTALMRFVDVMYAFP
                     SLLFVILIMAWRGPGLSNIFLAIGFTAWVTLARLTRAEFLSLREKDFVQAARAAGAGP
                     WRLVTRHLLPNALTPIIVAVTFGIPQAIFTEASLSFIGVGVPPPRPSWGQMVGQYFAN
                     VQSYWFLAVIPAVMIALVMLSFTFFGDGLRDALDPRMQRR"
BASE COUNT          661 a         1458 c         1392 g          751 t
ORIGIN      
        1 cgcatgccgg ccgccatgat ctttgagaag gtgcccaggt aggccgcccg gtccggcgcc
       61 agcgagtaga gcgtcggcac gcgcgtgccc tcgaagcgca gatcgaagta ggcgtcgtcc
      121 tcgacgatga agaagccgta ctggcgcgac agctcgatga tgcgccggcg ccgcgccagc
      181 gtggtcgtga cgccggtcgg gttctggaag gtcggaatca tgtagagcag cttggctgcg
      241 cggccctcgg ccgccagctg cgtcagcgtc gcctccagtg cggccgtgtc ggtgccctcg
      301 gcgtcgagcg gcaccggcac cacctcggcc gccacccgcc ggaaggcgtg caccgcgccc
      361 gaccaggtcg gctcctcgct caggatcacg tcgccggggt cgcagagcgc ctcgcagacc
      421 agccccagcg cctgaccagc gccggcggtg atcagcacgt tctcctcgct ggcctcgatc
      481 ccctggtcgc gcttcagctt cgtcagcagt tcctgcacca gctcggggta gccgcgcccg
      541 gcgccgtact gcagcgccca ctcgccgtcg cgctccagcg ccacccgggt ggcctcggtg
      601 atgtcgcgct tcggcaggct gccggggtcc gggaagccgg ccacgaagga gatcaggctg
      661 gcggaggcgc gcgggttggc caggcgcccg ccgccgagcc actgcgcccg gctgctcagc
      721 agcgactcga ccgttcggtt agcggtatcg gtcacagtct cccctttcgg cgtgtctcgc
      781 ggtcactcgt ctacgcggcg ctattgtagc gcgcacccgt gaagtcgcgc gggtatctac
      841 ctgtataatc ggcctcaata ccagccgggc acgacgagcg cagggttgcg gcgtgccgct
      901 ccggccgggt acgcgcaggc aacccaatcg caagcaggag gagtgcggcc gtgggggaga
      961 cccgcgagat ctttgacatg accatcatcg gcgggggacc gatcggcctc ttcgccgcct
     1021 tctacgccgg catgcgcggg ctgcgcacga aaatcatcga cagcctggag gagctgggcg
     1081 gccagctcat cgccgtctac cccgagaagt acatctacga cgtggctggc atccccaaga
     1141 tcctggccaa agagtacgtc accggcgcgg tcgagcaggg gctctcgacc ggcgccgtcg
     1201 ccgtgctcgg cgaggaggtg cgcctgctgg agcggctaga ggacgagcag ctgctgcacc
     1261 tagtcaccag caagggagac cattggtcgc gcaccgtgct gatcgcggcc ggggtgggcg
     1321 ccttcgagcc gaagcgcctg gaggcgccgg gggtcaacga actggagggc cggggcgtcc
     1381 actacttcgt gcgcgactta gagcagttcc gcgacaagga cgtggtgatc gtcggcggtg
     1441 gcgactcggc cgtcgactgg gcgctggcag tcgtgccgct ggcccggcgg ctgacggtgg
     1501 tgcaccgcag caagttccgc gcgcacgagc gctcgatcgc cgagctggag tcctccgcgg
     1561 cccagctcta cttccccttc tatgaggtca aggaggcgcg cggtaacggc aacctggagg
     1621 agatcgcctt ccagcacacc cgcaccgggg aagagcaccg cgtgccggcc caggccctga
     1681 tcgtggccat cggcttcggc ggcaacctcg gcccgctcaa ggagtggggc ttcgagatcg
     1741 agcgcaacat gatcgtggtc gacccgctga cgatggagac cggcatggcc ggcgtcttcg
     1801 cggccggcga catcatcacc cacccggtca agttcaagct gatcgccacc ggggccgccg
     1861 aggcggtcat cgccgtcaac cacgccgtca cctacatcga tcccaaggcg cggctcgacg
     1921 ctggccactc gaccaacctg atggagcggc gcgagcgcca gcgcgagcgt gagcatgagt
     1981 cgtagacgac gctgaacgaa tccgccaggc gaatgcggaa acaagcgcgg ttcgtcgacg
     2041 gccctgaacg aatccgccag gcaattttcg gagaagggga gccatttggc tccctttcgt
     2101 cgtatattgg gagaagcctc cctgtggtga tgccggtgcc gagaccagcg atgtgagcag
     2161 cgctccttgt gaaattgttg acagagtgcc ggtgacgatg ttacgctcgc agcacttcag
     2221 gtattcacgg atcagccagc gatcgggggc gtcttcggtc gtagcggcgt aggcagcgca
     2281 ccggcggtcg ttccgaaagg atacatgagg atgggtcgat tcattgcccg gcggctaatc
     2341 tggttggtac cgactatcct ggcgatgtcg ctgatcacct tcgccttgat gcacgcgaca
     2401 ccgggcagcc cgctccagcc gatggccgcc aacgccaacc cgctgccgcc cggcgctcag
     2461 gagaatctga aacacgcctg gggcctggat aaacccctgt ggcagcagta cgtgacattc
     2521 ctgtggaaag gcctccacct cgacttcggc acctcctacg tcttccgtac ccgcgcggtc
     2581 tccgacatcc tcaggcagac cttccccatc tccctcgaac tcggtctcat ggcgctcggt
     2641 ctcgcgatca tcatcggcat cccgctcggc atcctggccg ccgtcaacca gaacgggccg
     2701 atcgactatc tctccagctt catggcgacg ctgggcgtgg cggtgccgaa cttcgtgctc
     2761 ggcctcttca tgattgtgct cttcgtgctc atcttacata tcctgccgga caccggcggc
     2821 tggaacgggc cggtcgattg gatcatgccg gcgctggccc tcggactcgg cccgctcggc
     2881 atcctggcgc gctacactcg ctccagcatg gtcgaggtgc tgcggcagga ctacatccgc
     2941 accgcgcagg cgaagggtct cgcgccgagt cgggtggtga tcgcccacgc ggtcaagaac
     3001 ggcctgatcc cgccgttgac gatcctgggt ccgatgttcg cggccatcgg caccggctcc
     3061 ttcttcgtcg agacgatctt ccgcgtgcca gggatggggc aattcttcgt caccagcatg
     3121 atcaatcgcg actacccgat gatcatggcc gtgatcctcc tctacggcgt cttcctggcg
     3181 ttcatgaatc tggtcgtcga tgtttgctac ggcgttgtcg acccgcgtat tcgcctgagc
     3241 taggagcgca tgatggatgc tttggataaa ccgcaggccc aggacttgag ccagcttgag
     3301 gccaaatcgg cggaggtcgc atccggcgcg tcggcgctca gcgcggccgc gcgccagcac
     3361 cgcacgctct ggcgtgatgc cttccaccgc ctggtgcaga acaagctcgc cgtggccggt
     3421 ggcatcgtga tcctgatcat cgtggcgatg gcgctcttcg cgtccctggt ggccccggag
     3481 ccttacgcca cgccgaactt cgccgccatc aaccagttcc ccagctccgc gcactggctg
     3541 ggcaccgatg agctggggcg cgacttcctc agccggctga tctacggcgc gcaggtctcg
     3601 atgctggtcg gcctcggcgc ccagttcatc gtgctcttaa tcggcgtgcc gatcggcgcg
     3661 atcgccggct acgccggcgg caaggtcgac accgcgctga tgcgcttcgt cgacgtcatg
     3721 tacgccttcc cctccctgct cttcgtcatc ctgatcatgg cctggcgcgg gccgggcctg
     3781 agcaacatct tcctggccat cggttttacc gcctgggtca ccctggcgcg cctgacccgc
     3841 gccgagttcc tctccttacg tgagaaggac ttcgtgcagg ccgcgcgggc ggccggcgcc
     3901 ggcccctggc gcctggtcac gcgccacctg ctgcccaacg cgctgacgcc gatcatcgtg
     3961 gcggtcacct tcggcattcc ccaggcgatc ttcaccgagg ccagcctcag cttcatcggc
     4021 gtcggggtgc cgcccccgcg gccaagctgg ggtcagatgg tcggccagta tttcgccaac
     4081 gtgcagtcgt actggttcct ggccgtcata ccggccgtca tgatcgccct ggtcatgctc
     4141 tcgttcacct tcttcggcga cgggctgcgc gatgcgctcg atccgcgcat gcagcgacgc
     4201 taagcgagtg tgggtgtcag cggaacgctt cgactaggag gaggaggtca tcgcgcggca
     4261 gg
//
LOCUS       DASUBI010000436         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_730345, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000436 DASUBI010000000
VERSION     DASUBI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730345"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..649)
                     /locus_tag="VFU72_08360"
     CDS             complement(17..649)
                     /locus_tag="VFU72_08360"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5423536.1"
                     /translation="MERGTNPTRELEQALWSEGLARVAGVDEVGRGCLAGPVVAAACI
                     LPPGCGDIPGVRDSKLLTSKQRARVLTDIRQQALAIGLGAASRREIDRLNIRRASVLA
                     MRRALARLGQWDYALCDGLLPPELDPVCCRGVVHGDGLSLSIACASVVAKEARDALMR
                     RLAARHPDFGWEQNAGYGTAQHLDALARLGPTPHHRTSFEPVRLALETGT"
     gene            complement(721..1092)
                     /gene="rplS"
                     /locus_tag="VFU72_08365"
     CDS             complement(721..1092)
                     /gene="rplS"
                     /locus_tag="VFU72_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5423537.1"
                     /translation="MVDVIRSIEQEQIRTDIPPFGPGDTVRVHVKVVEGTRERIQPFE
                     GVVISRRSGGLNENFTVRRIASHGIGVERTFLLHAPRIDRIEVLRTGRVRRAKLYYLR
                     GRTGRAARIRERRRPAGSYLR"
     gene            complement(1177..3057)
                     /locus_tag="VFU72_08370"
     CDS             complement(1177..3057)
                     /locus_tag="VFU72_08370"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEU5423538.1"
                     /translation="MAHPFLERLVKGPILCDGAMGTQLYERGIPFDTCFDALNLSRPE
                     LVVEIHRDYIAAGAEIIETNSFGANRFKLEPHGLAERVREINRKAARNAREAREVSGQ
                     PVLVAGSVGPTGRTLAPIGTADPALARDAFREQVAGLLEGGVDLLIIETIPALAEMRE
                     AILAAREVSDLPIVAQLTFAQDGRTVAGNDATEVVAGLRELGVPVLGANCSVGPVHTL
                     EVLGALARALPDGTRLAGQPNAGWPMNVGGRVIYPSSPEYFADYARRAVEAGVAIVGG
                     CCGTTPVHIQSMRRVLDELGRVAPAPSVEVRAPRPVQLLAPEGPTKLSQKLGKEFVVS
                     VEVDPPKGLNPRKTLAGAQLLKDAGVDFINVADSPMARVRMSALALCYLIQNQVGVET
                     ILHFTTRDRNLMGLQSDLIGAHAIGVRNIIALTGDPPSLGDYPDATPVYDIDSIGLVK
                     VIKSFNAGTDAAGSSIGQLASFTVAVAADPTRPDLEEEAERLSRKIGAGADLVMTQPV
                     FALDTWLTFRRLYEERYGPIPVPVLLGVLPLQSHRHAEFLHNEVPGIRLTDEARERMR
                     LAGNEGRREGVKLARELLLEARDEVQGTYLMPSFGRYEVAAEVLEVLADRGLGAPTAL
                     PG"
BASE COUNT          458 a         1145 c         1057 g          456 t
ORIGIN      
        1 cccagcgcct gccggctcat gtgccggtct cgagcgcgag gcgcaccggc tcgaagctgg
       61 tgcggtggtg aggcgtgggt ccgaggcggg cgagcgcatc gaggtgctgg gcggtgccgt
      121 agccggcgtt ctgctcccag ccgaagtcgg ggtggcgcgc cgccaggcgg cgcatcagcg
      181 cgtcgcgcgc ctccttggcc acgaccgagg cgcaggcgat cgagagactc aacccgtcgc
      241 cgtggacgac gccgcggcag cagaccgggt ccagctccgg cggcagcagt ccgtcgcaga
      301 gcgcgtagtc ccactgcccc aggcgcgcca gcgcccgccg catcgccagc acgctggcgc
      361 gacggatgtt gagccggtcg atctcgcggc gcgaggccgc gcccagcccg atcgccagcg
      421 cctgttgccg gatatccgtc agtacccggg cacgctgctt cgaggtcagc agcttggagt
      481 cgcgcacgcc ggggatgtcg ccgcagccgg gcggcaggat gcaggccgcg gccaccaccg
      541 gcccggccag gcagccgcgc ccgacctcgt cgacgcccgc cacccgggct agcccctcgg
      601 accagagcgc ctgctctaat tctctggttg gattggtccc ccgctccatc tcccccggtt
      661 ccctggaaaa cgcgaaaggc ggtgcgctgc accgccttca gcagtcgagc ctcatcgccg
      721 ctagcgcagg tagctgcccg ccggccggcg ccgctcgcgg atacgcgcag cgcgcccggt
      781 gcggccgcgc aggtagtaga gcttggcgcg acgtacgcgg ccggtgcgca agacctcgat
      841 gcggtcgatg cgcggcgcgt gcagcaggaa ggtgcgctcg accccgatgc cgtgcgaggc
      901 gatccgccgc acggtgaagt tctcgttgag cccgccgctc cggcgcgaga tgaccacccc
      961 ctcgaacggc tgaatgcgct cgcgggtgcc ctcgaccacc ttgacgtgaa cccgcaccgt
     1021 gtcgcccggc ccgaagggcg ggatgtcggt gcgaatctgc tcctgctcga tgctcctgat
     1081 gacgtctacc attgctgtta ctccgaaatc cgcgcgtctt gcaaggcata atcgccccgc
     1141 ccaacgcggg accacgcgag tatagcagag ccctcgttag ccgggcaaag cggtgggcgc
     1201 acccaggccg cggtcggcca gcacctccag cacctcggcc gcgacctcat aacgcccaaa
     1261 ggacggcatc aggtaggtgc cctgcacctc gtcgcgcgcc tccagcagca gctcgcgcgc
     1321 cagcttgacc ccttcgcggc ggccctcgtt gcccgccagc cgcatgcgct cgcgcgcctc
     1381 gtcggtcagg cggatccccg gcacctcgtt gtgcaggaac tcggcgtggc ggtggctctg
     1441 cagcggcagc acgccgagca ggacgggcac cgggatcggc ccgtagcgct cctcgtagag
     1501 ccggcggaag gtgagccagg tgtccagcgc gaagaccggc tgggtcatca cgagatcggc
     1561 ccccgcgccg atcttccggc tcaggcgctc ggcctcctcc tccaggtcgg gccgggtcgg
     1621 gtcggcggcc acggccacgg tgaagctcgc cagttgcccg atcgaggagc cggccgcgtc
     1681 ggtgccggca ttgaacgact tgatcacctt gaccagcccg atcgagtcga tgtcgtagac
     1741 cggggtggca tcggggtagt cgcccaggct gggcgggtcg ccggtcagcg cgatgatgtt
     1801 gcgcaccccg atcgcgtgcg cgccgatcag gtccgactgc aggcccatca gattacggtc
     1861 gcgcgtggtg aagtgtagga tcgtttcgac acccacttga ttctggatca ggtagcaaag
     1921 cgccagcgcg ctcatgcgca cccgtgccat cgggctgtcg gccacattga tgaagtcgac
     1981 cccggcgtct ttgaggagct gggcaccggc cagcgtcttg cgcgggttca ggcccttggg
     2041 aggatcgacc tccacgctga cgacgaactc cttgcccagc ttctggctga gcttcgtcgg
     2101 gccttccggg gccagcagct gcaccggacg cggggcgcgc acctctacgc tcggcgccgg
     2161 cgccacccgt cccagctcat cgagcacccg ccgcatgctc tgaatgtgca ccggcgtcgt
     2221 gccgcagcag ccgccgacga tcgccacgcc cgcttccacc gcccgccggg cgtagtcggc
     2281 gaagtactcc ggcgacgacg ggtagatcac gcgcccgccg acgttcatcg gccagccggc
     2341 gttcggctgc ccggccagcc gcgtgccgtc cggcagagcg cgggccagcg cacccagcac
     2401 ctccagcgtg tgcaccgggc cgacgctgca gttggccccg agcaccggca ccccgagctc
     2461 acgcagcccg gccaccacct ccgtagcgtc gttgccggcg acggtgcggc catcctgcgc
     2521 gaaggtcaac tgcgccacga tcggcaggtc ggacacctcg cgcgccgcca ggatcgcctc
     2581 gcgcatctcg gccagcgcgg ggatcgtctc gatgatcagc agatcgacgc cgccttcgag
     2641 cagcccggcg acctgctccc ggaaggcgtc ccgcgccagc gccgggtcgg cggtgccgat
     2701 cggcgccagc gtacgcccgg tcggccccac cgagccggcc accagcaccg gctggccgct
     2761 cacctcgcgc gcctcgcgcg cattgcgcgc cgctttgcgg ttgatctcgc gcacgcgctc
     2821 ggccagacca tgcggctcca gcttgaagcg gttggcgccg aacgagttgg tctcgatgat
     2881 ctccgcaccg gccgcgatgt agtcgcgatg gatctcgacc accagctcgg ggcgtgagag
     2941 attaagcgcg tcgaaacaag tgtcgaaggg gatgccgcgc tcgtaaagct gggtgcccat
     3001 ggcgccgtcg cagaggatcg gacccttcac gagtcgttca aggaacggat gggccatgtt
     3061 tcaccccccg actcaggact gtacgtacaa attggccctt gccaaacgcc gggatt
//
LOCUS       DASUBI010000437         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_709005, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000437 DASUBI010000000
VERSION     DASUBI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709005"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..875
                     /locus_tag="VFU72_08375"
     CDS             453..875
                     /locus_tag="VFU72_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423539.1"
                     /translation="MTSEQHPSQNIENAVHQAKRGHLNYDFDGITLHLTGDRQLQFDY
                     EASDMRVAYRRSTPNSWQDIIGWMEKKGTFDNEFTPGEVVAIVEDLRSLKQSGTQFTN
                     NPDQAFQEAHQHRVRNNQQHQQLHQEILAASAQGPDAR"
     gene            complement(958..2106)
                     /locus_tag="VFU72_08380"
     CDS             complement(958..2106)
                     /locus_tag="VFU72_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423540.1"
                     /translation="MATRGPDDAARAARLAVGVVFLLQGLGAGNWFVRIPAVQAKLEL
                     SAGLLGFSLLGIAAGSLLSMPVTGWAVTRLGSRPLVRVAVVSSMLALALPGLAPLPLL
                     LFPALVVYGITAGMLDVSMNVQAVAVELRYRRPLLSSFHAMYSVGGLLGAALGGAVAA
                     LGISPAAHLTALGVVLALVGLLATRSLLPARSEVRAAGPAFARPSRALAALGLIALCA
                     LLCEGAMGDWSALYLDGTLGTGAGLAAAGYAAFSLAMAAGRLVGDRLTLRLGPPRLLR
                     LAGLLVLFGLGGGLLLRDVPAALVGFAATGAGLSAVFPVTVSLAGRGEANPGPAIAAV
                     STTGYLGFLAGPPLIGLLAGHSSLRLALGVVPLLGGVISLLGGTVRGR"
     gene            complement(2191..2952)
                     /locus_tag="VFU72_08385"
     CDS             complement(2191..2952)
                     /locus_tag="VFU72_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU5423541.1"
                     /translation="MTTYYDQNPYLTRFEWGPEGVRALAPGSDVVVIVDVLTFSTAVD
                     VAVGQGATVFPYRWRDASAERFAREQRAALAVSRGATSQEHPFSLSPASLRGLATGAR
                     LVLPSPNGATLSLLAAEAGAAVLAGSLRNAAATARVAAALGRRVTVIAAGERWPESDT
                     LRPSIEDLIGAGAILSVLDAPEASLSPEAEIALAAFAVVAPRLREHLDACASGRELRA
                     LGFAGDVAVAAELNASDSVPILRNGAYVRWAGPEE"
     gene            complement(2969..3280)
                     /locus_tag="VFU72_08390"
     CDS             complement(2969..3280)
                     /locus_tag="VFU72_08390"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEU5423542.1"
                     /translation="MTNPKRQKPAAERPRIPEFTSREEEAEFWDTHDIADYWDDLKPV
                     RVRFSQQLSEGITVRLDPETLARLRERARKLGVGPTTLARMWIIEQLRADELSEQSSQ
                     H"
     gene            complement(3636..5372)
                     /locus_tag="VFU72_08395"
     CDS             complement(3636..5372)
                     /locus_tag="VFU72_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5423543.1"
                     /translation="MPEQHSLTVERNVPVPMRDGTRLAADVYRPAGPGRRPTLLQRTP
                     YKKEQMTETNLILRALESGYAVVVQDVRGRFGSEGEFHCFVNERQDGYDTLEWLAAQP
                     WCDGNVGMFGQSYVGLTQWQAAMSGHPALKAIVPVVTASDYHADWVYQGGAFELSFNL
                     SWTLSNLVLDTIMRAEGGKGDDYQEVLRLVDDMHRQFEILPLADHPLLQKYAPYYGEW
                     LAHPTYDEFWQRLDVSTMHDQLTLPAFNIGGWYDIFLGGTIANFTGRRAKAPTSQARA
                     AQRLLLGPWNHSGMRLGNPIGDIDFGVGSTGAEYEVETLRMRWYDHWLRGADNGIDDE
                     PPVRVFVMGARRWRTAEDWPLPGTDFQPWFLHSAGKANSLHGDGGLGREAPANEPPDS
                     YASNPRNPVPTRGGGLCCHNVYSLGGAYDQRLVEAREDVLVYSSAPLETPIEVTGPVK
                     VVLYAASSAPDCDFTAKLVDVFPCGQALNLTDGILRARYRDSMRAGTPLTPGEVTKFE
                     IDLFATSNLFQKGHQIRVEIASSNFPRFDRNPQTGAPPGQATLEELTSALQTVHHSTE
                     YPSHIVLPVVPA"
BASE COUNT          920 a         2018 c         1933 g          798 t
ORIGIN      
        1 gtagaggtcg cgccagcggg aatcgccggt cgctgtctcc gcgctcagat tcatcgctcc
       61 cctactcctc cctggagacg ctgacgccgc ccaccatgtg cctcttcatg atcctccctg
      121 ccgcctggcc gggcaagaag caacctgacc gctgacgcac gcggatactc cctcaaccag
      181 ccgcgcggtg ccaacccatg cgccgcgcga gaagaacggc ggcacgaata tcgtcttgcg
      241 gtaatagctt gtgcgtggca tggattctgt gggccaatgg cggcagggtc gcgagcctat
      301 tcgactccgc ccgaaatcga ccggagagcc ggcgacatca aggtctacct gtccgattcg
      361 gtctgactgc atggccagat cagcgacgag gtgagcggga tccggcaccg gcacgacgat
      421 gagtcgggag cacagcattc aggaggttgc gcatgaccag cgagcagcac ccgagccaga
      481 acatcgagaa cgccgtccat caggcgaagc ggggccatct caactacgac ttcgacggca
      541 tcacgctcca cctgaccggc gaccgccagc tccagttcga ctacgaggcg agcgacatgc
      601 gggtggccta ccggcgcagc accccgaact cctggcagga catcatcggt tggatggaga
      661 agaagggaac cttcgacaac gagttcaccc ccggcgaggt ggtggccatc gtcgaagacc
      721 tgcgctcgct gaagcagagc ggcacgcagt tcaccaacaa ccccgaccag gcgttccagg
      781 aggcgcacca gcaccgcgtc cggaacaacc agcagcatca gcagctgcac caggagatcc
      841 tggccgcctc agcgcagggg ccagacgcgc gctagcgcta cgcccgtcgg actgtctgcc
      901 ggcatcggtg ccgggtgtcg cgcggtaccc gcgctccacg gaacgcggca tccgccccta
      961 ccgcccgcgc accgtgccgc cgagcagcga gatcacgccc ccgagcagtg gcacgacccc
     1021 cagcgccagc cgcagcgagc tgtggcccgc caagaggccg atcagcggcg ggccagccag
     1081 gaagcccagg tagccggtgg tcgagacggc cgcgatggcg gggccgggat tcgcttcccc
     1141 gcgtccagcc aggctgacgg tgaccgggaa gaccgccgag agcccggcgc cggtggcggc
     1201 gaagccgacg agcgcggccg gcacatcccg cagcagcagc ccgccgccga gaccgaacag
     1261 caccagcagc cccgccagcc gtagcagccg cggggggccg aggcgcagcg tcagccgatc
     1321 gcccaccagc cgcccggccg ccatcgccag cgagaaggcg gcgtagccgg ccgcggccag
     1381 ccccgcaccg gtgcccagcg tgccgtccag atagagcgcg ctccagtcgc ccatggcgcc
     1441 ctcgcagagc agcgcgcaca gggcgatcag ccccagcgcg gccagcgcgc ggctggggcg
     1501 ggcgaaggcc ggaccggcgg cgcgcacctc ggagcgcgcc ggcagcaggc ttcgcgtcgc
     1561 gagcagcccg accagcgcca gcacgacccc gagcgcggtc aggtgcgccg ccgggctgat
     1621 ccccaacgcc gccaccgccc cgcccagcgc cgcgccgagc aacccgccga cgctgtacat
     1681 ggcgtggaag gaggacagca gcggtcgccg gtagcgcagc tcgaccgcca ccgcttgcac
     1741 gttcatcgag acatcaagca tgccggcggt gatgccgtag acgaccaggg ccgggaagag
     1801 caggagcggc agcggcgcca acccggggag cgccagcgcc agcatcgagc tgaccacggc
     1861 cacgcgcacc agcggccggc tgcccaggcg ggtcacggcc cagccggtga ccggcatcga
     1921 gaggagcgag ccggccgcga tccccaacag cgagaagccg agcagcccag cgctcagctc
     1981 gagcttggcc tgcaccgccg ggatgcgcac gaaccagttg ccggcgccga gcccctgcaa
     2041 gaggaagacg acgccgacgg ccagccgcgc ggcccgcgcc gcgtcatcgg gcccgcgcgt
     2101 ggccatcgct gcgccgccac cggcttccgg ccggtcccca ctccgcgtca agtttggtga
     2161 ggctctctcc ccgccatggt cgcgccgcga ctactcctcc ggcccggccc agcggacgta
     2221 ggcgccgttc cgcaggattg gaacggagtc actggcgttc agctcggccg ccaccgcgac
     2281 atccccggcg aagcccagcg cgcgcagctc gcgtcccgag gcgcacgcat ccaaatgctc
     2341 ccgcagccgc ggggcaacga cggcgaaggc ggccaacgcg atctcggcct cgggcgacag
     2401 gctggcctcg ggcgcgtcga gcacgctgag aatcgccccc gcgccgatca aatcttcgat
     2461 cgagggacgc agcgtatcgc tctccggcca gcgttcccca gcggcgatga ccgtcacccg
     2521 gcgtcccagc gcggccgcga cccgcgcggt cgccgccgcg ttgcgcaagc tgcccgccag
     2581 caccgccgcg ccggcttcag ccgccagcag cgacagggtg gcgccgttag gcgaggggag
     2641 caccagccgc gcgccggtcg ccagccctcg cagggacgcg ggagagagcg agaaggggtg
     2701 ctcttggctg gtcgcgccgc ggctgaccgc cagcgccgcg cgctgctcgc gagcgaagcg
     2761 ctcggccgag gcgtcgcgcc agcggtaggg gaagacggtc gcgccctggc ccaccgccac
     2821 gtcgacggcg gtgctgaagg tcagcacgtc gacgatcacc accacgtcgg agccgggcgc
     2881 cagagcccgc accccctccg gcccccactc gaagcgcgtc aggtacggat tctggtcgta
     2941 gtacgtcgtc acgccaccca gacaagcatc agtgctggct cgattgttcc gaaagctcgt
     3001 ccgctcgcag ctgctcgatg atccacatcc gcgccagcgt cgttgggccg acacccagct
     3061 tgcgtgcgcg ctcacgcagc cgcgccagcg tctcgggatc gagccgcacg gtgatccctt
     3121 cagaaagttg ctggctgaac cgtacccgca cgggtttgag atcgtcccag tagtccgcga
     3181 tatcgtgcgt gtcccagaat tcggcttctt cctctcgact ggtgaactcg ggaattcgtg
     3241 gacgctcggc tgccggtttc tggcgctttg ggttcgtcat cgttattgct ccgcctggta
     3301 ctgctcgtac agccggcgtt ctcttcggct cgcaggtcga gccgttagcg gaaagaagac
     3361 accgggctcg accggctcga gaacgacagc cagcattcgc ccggtcgtcg ttggcccgat
     3421 cagcacgagt ctggcttgat agccctgccg gacgatgtag ggaccgcgac agacttcctc
     3481 gacttcgtcc ggcgttactc catgcctggc aatgtgcgcg atattccacg gatcccagcg
     3541 caggtgccgc accgtgatca tggattagtt gtaacacaat gcgttacacg cggagccatt
     3601 gctgcgcggc cattcctcgt tcgtcctcgc acgcctcacg ccggcaccac cggcaacaca
     3661 atgtgcgacg ggtactcggt cgagtggtgc accgtctgca gcgcgctggt cagctcctcc
     3721 agagtggcct ggccgggcgg cgcgcccgtc tgcgggttgc ggtcgaagcg cgggaagttg
     3781 ctgctggcga tctcgacccg tatctggtgc cccttctgga acaggttgct ggtggcgaag
     3841 aggtcgatct cgaacttggt cacttcgccg ggggtcagtg gcgtccccgc gcgcatcgag
     3901 tcgcggtagc gggcgcgcaa gatgccgtcg gtcaggttga gcgcctggcc gcaggggaag
     3961 acgtcgacca gcttggcggt gaagtcgcag tcgggcgcgc tggaggcggc atagagcacc
     4021 accttgaccg ggccggtcac ctcgatgggc gtctccagcg gcgcgctgct gtaaaccagc
     4081 acgtcctcgc gcgcctcgac caggcgctgg tcgtaggcgc caccgagact gtagacgttg
     4141 tggcagcaga ggccgccgcc gcgcgtcggc accgggttgc gcgggttgct ggcgtagctg
     4201 tcgggcggct cattggcagg cgcctcgcga ccgaggccgc cgtcgccgtg cagggagttg
     4261 gccttgccgg cgctgtgcag gaaccagggc tggaagtcgg tgccgggcag cggccaatcc
     4321 tcggccgtgc gccagcggcg ggcgcccatc acgaagacgc gcaccggcgg ctcgtcgtcg
     4381 atgccgttgt ccgccccgcg cagccagtgg tcgtaccagc gcatgcgcag cgtctcgacc
     4441 tcgtactcgg ccccggtcga gccgacaccg aagtcgatgt cgccgatcgg gttgcccagg
     4501 cgcatgccgg agtggttcca gggaccaagc agcagccgct gggcggcgcg cgcctggctg
     4561 gtgggcgcct tggcgcgcct gccagtgaag ttggcgatcg tgccgccgag gaagatgtcg
     4621 taccagccgc cgatgttgaa ggcaggcagg gtcaactggt cgtgcatggt cgagacgtcg
     4681 agccgctgcc agaactcgtc gtaggtgggg tgcgccagcc actcgccgta gtagggcgcg
     4741 tacttctgca gtagcgggtg atccgccagc ggcaagatct cgaactggcg gtgcatgtcg
     4801 tcgaccaggc gcagcacctc ctggtagtcg tcgcccttgc cgccctccgc gcgcatgatc
     4861 gtgtcgagca ccaggttgga cagggtccag gagaggttga aggagagctc gaaggccccg
     4921 ccctggtaga cccaatcggc gtggtagtcg ctggcggtca ccaccggcac gatggctttg
     4981 agcgcggggt ggccgctcat agccgcctgc cactgggtca acccgacgta ggactggccg
     5041 aacatgccca cgttgccgtc gcaccagggc tgggccgcca gccactccag cgtgtcgtag
     5101 ccatcctggc gctcgttgac gaagcagtgg aactcgccct ccgagccaaa gcgcccgcgc
     5161 acgtcctgca cgacgaccgc gtagcccgac tccagcgcgc gcaggatcag gttggtctcg
     5221 gtcatctgct cctttttgta cggggtgcgc tgcagcaagg tggggcggcg gcccggtccg
     5281 gccgggcgat agacatcggc ggccagccgc gtgccgtcgc gcatcggcac cggcacgttg
     5341 cgctcgacgg tcaggctatg ctgctcaggc atcggcttct tcccctctcc ctcttcaacc
     5401 atcggaagga cgcggccatg ctatgcaggc gaccgagcgt cgtcaagcac gcgcgccgcg
     5461 ctctcacgga gttttcagga tcggccgcta tgctcatgaa cggagttggc tgagccgggg
     5521 cacgaggagc ttcgccggat ggccgtgagc gctcccgaga tcgtgatgga agccggacca
     5581 ggcagcgcgg ccgagcgagt cgcggccgtc tcgcgctggg agcgccccgc gctggccgcg
     5641 ctgctcggtc tcaccgccct gctctacct
//
LOCUS       DASUBI010000438         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_686920, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000438 DASUBI010000000
VERSION     DASUBI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686920"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..587)
                     /locus_tag="VFU72_08400"
     CDS             complement(96..587)
                     /locus_tag="VFU72_08400"
                     /inference="COORDINATES: protein motif:HMM:NF045311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5423544.1"
                     /translation="MASYAQQVGERLRNVRVQKGLSLHDVEIQSGGEFKASVLGAYER
                     GERSISVNRLQRLAMFYAVRVDGMLPRIGPEPTGSAAWAMTLPNEGLCIDLPALALRR
                     EPEARVLEQFVTILQMQRGDYGGKMLTIRREDVRVLAAVLDRSPEELLERLSALGLRR
                     RAG"
     gene            complement(787..1701)
                     /gene="nrfD"
                     /locus_tag="VFU72_08405"
     CDS             complement(787..1701)
                     /gene="nrfD"
                     /locus_tag="VFU72_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5423545.1"
                     /translation="MTGGDGADFRSYYGHPILKEPVWTWEIPWYFFLGGMAGASAPLA
                     FAARLAGNHRLARAALTTGAASLAAGPVLLTADLGRPGRAHHMLRVFKPTSPMNLGSW
                     QLAAISPALWGAAVADRLGIMPTLARTAEGVAAVLGPGLATYTAVLLADTAVPVWHEA
                     GRELPFVFASSAAASAGAAACLLIPPGDAGPARRLAVGAAAAELAAVAVTERRLGELA
                     EPYRLQPARRFVRLGKGLSLAGALVLGLAGRRRLAAAVGGALLLAGSACQRQAVVRAG
                     RLSARDPKYVVKPQRQRLRQLGAARSSG"
     gene            complement(1698..>2109)
                     /locus_tag="VFU72_08410"
     CDS             complement(1698..>2109)
                     /locus_tag="VFU72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5423546.1"
                     /translation="CPFGVIDRREDDGRAWKCTLCYDRTRDGLEPACAKACPTDSIQY
                     GQLDELRERARQRLATLHQLGVADANLYLADEQDGIKGAGAFFLLLDEPEAYGLPPGP
                     VSTTRDVGAFWKAAGMAAGALVAGVAAAFIGGRG"
BASE COUNT          301 a          754 c          786 g          268 t
ORIGIN      
        1 cccaggtctc gtcgtcggga tgcgcgaagc agcccacgac ggtcagcgga tggtccaagg
       61 ttcctccagc tcagcgtccg cggatccccg agagcctacc cggccctgcg gcgcaggccg
      121 agcgctgaga ggcgctcgag cagctcctcc ggcgagcggt cgaggaccgc cgccagcacg
      181 cggacgtcct cgcgccggat ggtgagcatc ttgcccccat agtcgccacg ctgcatctgc
      241 aggatcgtga cgaactgctc gaggacgcga gcctccggct cgcgtcgcag cgccagcgcc
      301 ggcaggtcga tgcacagccc ctcgttgggc agggtcatcg cccaggcggc cgagccggtc
      361 ggctccgggc cgatgcgcgg cagcatgccg tccacgcgca cggcgtagaa catcgccagc
      421 cgctggaggc ggttgacgga gatgctgcgc tcgccgcgct cgtaggcgcc gagcacgctg
      481 gccttgaatt cgccgcccga ctggatctcg acatcgtgca gcgaaaggcc cttctgtacc
      541 cggacgttgc ggagccgctc gcccacctgc tgcgcgtagg acgccatccc aagctccctc
      601 cgagcgccga tggtggcggg actctagcat tgccccctaa caccttcaat ctccgaaggc
      661 gggcaataaa ccctacgcgg gtgtatcggg gcgttcgcgc ccccacgccc aggcctcgga
      721 gacggcctgg gcgggccgcc ggggtacccg gggaggcgcg ggcgggtcag cggcggatgg
      781 gggcagtcag ccggaggacc gggctgctcc aagttgccgc aaccgctgcc gttgtggctt
      841 gaccacgtac tttgggtctc gcgcagacag ccggcccgca cgtaccactg cttggcgctg
      901 gcaggcagag ccggcgagca ggagcgcgcc accgaccgca gcggctagcc gacggcgccc
      961 ggcgagcccg agcacgaggg caccggccag gctgaggccc ttgccgagac ggacgaagcg
     1021 gcgtgctggc tggagccggt acggctcggc cagctcgcca aggcgccgct ccgtgactgc
     1081 gacggcggcc agctccgcgg cggcggcgcc gacggcgagg cggcgggcgg ggccagcgtc
     1141 ccctggtggg atgagcaggc acgcggctgc gcctgcggac gcggcggcgc tgctggcgaa
     1201 cacgaacggc agctcgcggc ccgcctcgtg ccacaccggc acggcggtgt ccgcgagcag
     1261 gacggccgtg taggtggcga ggccggggcc aaggacggca gcgacgccct cggcggtgcg
     1321 ggccagcgtg ggcatgatgc cgagccggtc ggcgacggcc gcgccccaca gcgctgggct
     1381 gatggctgcg agctgccagc tccccaggtt catcggcgag gttggcttga agacccgcag
     1441 catgtggtgg gcacgcccgg gccggccgag gtcggcggtg agcagcaccg ggccggcagc
     1501 gagcgaggcc gcgccggtgg tgagcgctgc gcgggcgagc cggtggttgc cagccaggcg
     1561 ggcggcgaag gcgagcggcg cggacgcgcc cgccatcccc ccgagaaaga agtaccaggg
     1621 gatctcccag gtccagaccg gctccttcag gatcggatgg ccgtagtagg agcggaagtc
     1681 ggccccgtcg cctccggtca tccgcgaccc ccaatgaatg ccgccgcaac tccggccacc
     1741 agcgcgcccg ccgccatgcc tgcggccttc cagaaggccc ctacgtcccg cgtggtgctg
     1801 accgggccgg gcggcagccc gtaggcctcc ggctcgtcca gcaggaggaa gaacgcgccg
     1861 gctcccttga tgccgtcctg ctcgtcggcg aggtagaggt tggcgtctgc taccccgagc
     1921 tggtgcaggg tcgccagtcg ttgccgagcg cgctcgcgca gctcatcgag ctggccgtac
     1981 tggatcgagt cggtcgggca cgctttggcg caggccggct cgagcccatc ccgcgtgcgg
     2041 tcgtagcaga gcgtgcactt ccacgcccgg ccgtcgtcct cgcgccggtc gatcacgccg
     2101 aaggggcag
//
LOCUS       DASUBI010000439         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_899515, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000439 DASUBI010000000
VERSION     DASUBI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899515"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /gene="fabF"
                     /locus_tag="VFU72_08415"
     CDS             <1..1289
                     /gene="fabF"
                     /locus_tag="VFU72_08415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5423547.1"
                     /translation="FCASCGRARDGQQPRRRVVVTGVGAVSPLGLSAEATWQGLLAGE
                     SGIRRIERFDPAELPVQIAGEVRDFRLGDWGIDPKGVRQMALFSQFAVEAAGMALRDA
                     GLGVEAFDPDRASVVVGSAAGGMATILEAQEVAQRRGLMRTSPFFMTTFPLNLPSYHV
                     AQTFRFLGPSLSISTACATGAQAIGEAAQFIRQGQADLALAGGTEHDVYPLFIAGFAV
                     QKAASTLNEAPERASRPFDATRQGFVIAEGAAVLLLESLEHALARGATIYAEFLGYGS
                     SNDGFHPIAPDPVGRGAALAMHAALADGGLCVAQVDYINAHAASTPLGDKAETVAIKR
                     ALGERAYAVAVNSTKSMIGHLMGAAGAMEAMATVLSLRDQVLHPTINYETPDPECDLD
                     YVPNEARPATIEVALSNSFGLGGQNACLAFARYRPA"
     gene            1406..2038
                     /locus_tag="VFU72_08420"
     CDS             1406..2038
                     /locus_tag="VFU72_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEU5423548.1"
                     /translation="MVLGNDILSGRIARRAAQIRPGKADVFTILEGQYLQITDMEGKQ
                     VAELVAFNADDHDEVLSTAHTRAINNSIMLVKGMNLYSNRRNAMFVVVEDTVGRHDLL
                     MPSCDLRRYENDYGLSEHPGCREALDLELRAAGHEMPVDRIPDPVNFFMNVGLRARGE
                     LEVREPLSERNDYVLLRALMPMIVGVAACPQEQSAATGFNPSDILIRVYM"
     gene            complement(2256..3083)
                     /locus_tag="VFU72_08425"
     CDS             complement(2256..3083)
                     /locus_tag="VFU72_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5423549.1"
                     /translation="MAENAQAVSWSTVEGIIYRAESSGGVVGAAIVAPNEDAFEYHGD
                     RRFRAASIMKIPLMIEIFRQIDRGERALDDTHTLKEAEKAPGSGVMLHLHDGMVFTLH
                     DLLYLMISISDNTATNLLIDMAGMARVNQTMRELGMVNSTLGRKMLGRPVEDEKQENW
                     ATPDDYVTAVQAILDGQAASAESCKQMIALLELQQNRRRIARHLPEEKGIRWGTKTGS
                     IKGVTNDVGFIMTPQQTLVLAIFCENFPDQHAAEAAIGAISRAAMAATGIVKPLYTS"
     gene            3449..4705
                     /locus_tag="VFU72_08430"
     CDS             3449..4705
                     /locus_tag="VFU72_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423550.1"
                     /translation="MRRLSAYQVYLIICGASALFFATITTVTGVYLVTVAHFTPFELL
                     LVGTVLEATCFLMELPTGIVADVYSRRLSVIIGLALIGLGFMLEGSMPRLAPVLIAQV
                     IWGIGATFVSGAQEAWIADELGQEGVGIGRVYLRGAQADQLGALLGIGLSVALASLHI
                     GLAIVVGGALHVALALALLAIMPERHFQRAEHGSHNPFHAMRATAAGGLRAVRGRPVL
                     ITILAIGAFFGASSEAFDRLWEAHFLTDIGLPSLGGLPFVVWFGIITAGEMLLSIGAT
                     EIARRRVDSDSHAGAARALLAINTLMIAGMLAFGLSQGFVAALAAYWAVRLLRRTRMP
                     LYTAWVNQGINPRVRATVLSLASQADALGQVGGGPLLGLIGSAVSIPAALISAGLFLA
                     PANFLFTRTLRHQEPVGAVSEAEGGG"
     gene            5016..5735
                     /locus_tag="VFU72_08435"
     CDS             5016..5735
                     /locus_tag="VFU72_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU5423551.1"
                     /translation="MTLDLRDFDVLTFDCYGTLIDWESGILAALRPLLTRHGVALSDD
                     QVLEQFGALESHAELPPYRSYREVLARVVDGFGQQHGFTPTPDERAALAGSVPDWPAF
                     PDTTAALKSLARQYKLAILSNVDDDLFAGSARRLGVDFAAVVTAQQVGSYKPDPRNFA
                     RAIERVGVSRARLLHVAQSLFHDIAPAKAAGLATVWVNRRHDRPGFGATPPASAEPDL
                     EVPDLATLARLVEAAPRPGDQ"
     gene            5916..>6320
                     /locus_tag="VFU72_08440"
     CDS             5916..>6320
                     /locus_tag="VFU72_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU5423552.1"
                     /translation="MDKMDKRTGHDSAGWDLDTRLIHGSQEPDPATGALVPPIHPAST
                     FVRRPIDDDATYRYARGDNPTRAALETILAELEHGVAASAFGSGMAAVTAALQLLSGG
                     DHAIVGYDCYLTTYDFFANDLPRYGISADFVDL"
BASE COUNT          931 a         2227 c         2112 g         1050 t
ORIGIN      
        1 atttctgcgc cagctgtggc cgggcgcgcg acggtcagca gccacgtcgc cgggtggtgg
       61 tgaccggtgt cggcgcggtc tcgccgttag ggctgtccgc cgaggcgacc tggcaggggc
      121 tgctggccgg cgagtcgggt atccggcgca tcgagcgctt tgatccagcc gaactgccgg
      181 tgcagatcgc cggtgaggtg cgcgactttc gcctgggcga ctggggcatc gaccctaagg
      241 gtgtgcgtca gatggcgctc ttctcccagt tcgcggtcga ggccgcgggg atggcgctgc
      301 gggacgccgg gctgggggtg gaagcattcg accccgaccg cgcgtcggtc gtcgtcggca
      361 gcgccgccgg cgggatggcg acgatcctgg aggcgcagga ggtcgcgcag cggcgtgggc
      421 tgatgcgcac ctcgcccttc ttcatgacga ccttcccgct caatctgccg tcgtaccacg
      481 tggcccagac cttccgcttc ctcggaccca gcctgtccat ctcgaccgcc tgtgccaccg
      541 gcgcccaggc gatcggcgag gcggctcagt tcatccgcca ggggcaggcc gacctggcgc
      601 tggccggcgg caccgagcac gatgtctacc cgctcttcat cgccggcttc gcggtccaga
      661 aggcggcctc gacgctcaac gaggcgcccg agcgggccag ccgccccttc gacgctactc
      721 gccagggctt cgtgatcgcc gagggtgccg cggtgctgct gctcgagtca ttggagcacg
      781 cgctggcgcg cggagccacc atctacgccg agttcctcgg ctacggcagc agcaacgacg
      841 gcttccaccc gatcgcgccc gatcccgtgg gacgcggtgc ggcgttggcg atgcatgccg
      901 cgctggccga cggcggcctc tgcgtcgcgc aggtcgacta catcaacgcc cacgccgcca
      961 gcacgccgct cggcgacaag gccgagacgg tcgcgatcaa gcgggcgctg ggggagcggg
     1021 cgtacgccgt ggcggtcaac tcgaccaagt cgatgatcgg tcacctgatg ggcgcggccg
     1081 gcgcgatgga ggccatggcg acggtgctca gcctgcgcga tcaggtgctg cacccgacga
     1141 tcaactacga gacaccggac ccggagtgtg atctcgatta cgtgcccaac gaggcgcgcc
     1201 cggcgacgat cgaggtggcg ctgtcgaact cctttgggct gggcggtcag aacgcctgcc
     1261 tcgccttcgc ccgctaccgt cccgcgtaac tgtgctcccc tcacccccgc gggggagagg
     1321 ggctgggggt gagggcgcct tggggctggg gtgagggaat tcctgagcgt gagggcctgc
     1381 cacctagaaa gcagagcgtc gaaccatggt cctcggcaac gacatcttga gtggacgtat
     1441 cgcccgccgc gcggcccaga tccggccggg caaggccgac gtcttcacca tcctggaggg
     1501 ccagtatctc cagatcaccg atatggaggg gaagcaggtc gccgagctgg tcgccttcaa
     1561 cgccgacgac cacgacgaag tcctctcgac cgcgcacacg cgcgccatca acaactcgat
     1621 catgctggtc aagggaatga acctctacag caaccggcgc aacgccatgt tcgtggtggt
     1681 ggaggacact gtcggccgcc acgacctgct gatgccctcc tgcgatctgc gccgctacga
     1741 aaacgactac gggctgtccg agcatcccgg ctgccgtgag gcgctcgatc tggagctgcg
     1801 ggcggccggg cacgagatgc cggtcgatcg catccccgac ccggtcaact tcttcatgaa
     1861 cgtggggctg cgagcgcgcg gcgagctgga ggtgcgcgag ccgctctccg agcgcaacga
     1921 ctacgtgctg ctgcgggcgc tgatgccgat gatcgtgggc gtcgccgcct gcccgcagga
     1981 gcagagcgcg gccaccggtt tcaaccccag cgacattctg atccgggtct acatgtaggc
     2041 gcagagtggc gcggcgactt gcgacgacgc tccgtgccgc gtggggacgg atccttcgac
     2101 aagctcagga tgacaagggg aggggatgag gcgtacgacg acgatcagca tcgcccgacg
     2161 ccgatcctcc tcgtcagccc cccacgaacc ctgtcatcct gagcgcagtc gaagggtccg
     2221 tcttcgctgg cactcaacgg gctcgaaggc ccgccctatg acgtatacag cggcttcacg
     2281 atccctgtcg cggccatggc ggcccggctg atcgcgccga tggctgcttc ggcggcgtgc
     2341 tggtcgggga agttctcaca gaagatcgcc agcaccagcg tttgctgggg cgtcatgatg
     2401 aagccgacgt cgttggtgac gcccttgatg ctgccggtct tggtgcccca gcgaatcccc
     2461 ttctcctcgg gaagatgccg cgcgatgcgc cgccggttct gctgcagctc cagcagcgcg
     2521 atcatctgct tgcaggactc cgccgaggcc gcctgcccgt cgaggatggc ctgcaccgcc
     2581 gtcacgtagt cgtccggggt ggcccagttc tcctgcttct catcttcgac cgggcggccg
     2641 agcatcttgc gccccagcgt cgagttgacc atgcccagct cgcgcatggt ctggttgacc
     2701 cgcgccatgc cggccatgtc gatcagcaga ttcgtcgcgg tgttgtcgct gatcgagatc
     2761 atcaggtaga ggaggtcgtg cagggtgaag accatgccgt cgtgcaggtg cagcatcaca
     2821 ccgctgcccg gcgctttctc ggcttccttg agcgtgtgcg tgtcgtcgag cgcgcgctcg
     2881 ccccggtcga tctggcggaa gatctcgatc atcaggggga tcttcatgat gctggcggcg
     2941 cggaagcgtc ggtcgccgtg gtattcgaag gcgtcctcgt tgggcgccac gatggccgcc
     3001 ccgactaccc cgccgctcga ttcggcccgg tagatgatcc cctcgaccgt gctccagctc
     3061 actgcctgcg cgttctcggc catgcctgtc tcccactctc gctcaagccg cctgccaaca
     3121 tgactccggc cataatagag ccagcctgcc tgcggggcca aaccccggag cgcgctaccc
     3181 cgccgccatg ctagacttcc gcctgttcgt gagctcgaca gctcagtccg aggaggatgc
     3241 gatgtcaatg cttgccaccg ctcgccagca ggcgcgagac gccgtcgggg aaccggccgg
     3301 ctagaccggg gcaagcaccg gagcgactgc gcggcgctcg cgcggccgtt ccgcttccgg
     3361 gcactccacc tccagcctcc accgcgctgc tggcaaccgc tgatcgtctt cccagcgtta
     3421 ttggcctgcc atcgaatgcg ggagcagcat gcgcagacta tccgcctatc aggtctatct
     3481 gatcatctgc ggcgcttcgg cgctcttctt cgccaccatc accaccgtca ccggcgtcta
     3541 cctggtcacg gtggcgcact tcaccccctt cgaattactg ctggtcggca ccgtgctgga
     3601 ggcgacctgc ttcctgatgg agctgccgac cgggatcgtg gccgacgtat acagccgccg
     3661 cctctcggtc atcatcgggc tggccttgat cggcctcggc ttcatgctcg aggggagcat
     3721 gccccggctg gcgccggtcc tcatcgccca ggtcatctgg ggcatcggcg ccaccttcgt
     3781 cagcggcgcc caggaggcct ggatcgccga cgagctgggg caggaggggg tgggaatcgg
     3841 gcgcgtctac ctgcgcgggg cgcaggccga ccagctcggc gcgttgctcg gcatcggcct
     3901 gagcgtggcg ctggccagtc tgcacattgg cctggccatc gtggtgggcg gtgcgctgca
     3961 cgtcgctctg gcgctggcgc tgctggcgat catgcccgag cgccacttcc agcgcgccga
     4021 gcacggctca cacaatccgt ttcacgccat gcgcgccacc gccgccgggg ggctgcgcgc
     4081 ggtgcgcggc cgcccggtgc tgatcaccat cctggctatc ggcgccttct tcggcgcctc
     4141 cagcgaggcc ttcgaccggc tctgggaggc gcacttcctg accgatatcg gactgcccag
     4201 cctcggaggg ctccccttcg tggtctggtt cggcatcatc acggcaggtg agatgctctt
     4261 gagcatcggc gccaccgaga tcgcgcggcg ccgggtggac agcgatagtc acgccggcgc
     4321 ggcccgcgcc ctgctggcga tcaacacgct gatgatcgcg gggatgctcg ccttcgggct
     4381 ctcccagggc ttcgtcgccg cgctggcggc ctactgggcg gtgcgcctgc tgcgccgcac
     4441 cagaatgccg ctctacaccg cctgggtgaa ccaggggatc aacccccgcg tgcgcgccac
     4501 cgtcctgtcg ctggccagcc aggccgatgc gctgggccag gtcggcggcg gcccgctgct
     4561 cggactgatc ggcagcgccg tctcgatccc ggccgcattg atcagcgccg gcctgttcct
     4621 cgcgcccgcc aacttcctct tcacccgcac cctgcgccac caggagccgg tcggtgcggt
     4681 tagcgaggcg gagggaggtg gctgacgata ggaattggac gtagtcgagg acggatcctt
     4741 cgacaagctc agagcctgcc ctgagcctgc cgaagggatg accgggtaaa ggatgcggcc
     4801 taccggacga gccgaatcct cgtcgtaggc ccctccctct ccctgtcagc ctgatcccag
     4861 tcgaaggatc cgtgctctcc aggcacccaa ccgagtcgac cggcacgtac ccgccttctg
     4921 ccggcagcct tcggctcaca tggattgctc gccactcggc agcgggctat gctgtcctcc
     4981 atggattggc tgcatcgtga gacacgggag cacggatgac gctcgatctg cgggacttcg
     5041 acgttctgac cttcgactgc tacggcacgc tgatcgactg ggagtccggc atcctggccg
     5101 cgctgcggcc gctcctgacg cggcacggcg tcgcgctgtc cgacgatcag gtgctggagc
     5161 agttcggcgc gctggagtcg cacgccgagc tgccccccta ccgctcctac cgcgaggtgc
     5221 tggcgcgcgt ggtggacggc ttcggtcagc agcacggctt cacacccacg ccggacgagc
     5281 gcgcggcgct ggccgggtcc gtgcccgact ggcccgcctt ccccgacacg acggccgcgc
     5341 tcaagtcact ggcccggcag tacaagctgg cgatcctctc caacgtcgac gacgatctct
     5401 tcgccggctc ggcgcggcgg ctgggggtcg acttcgcggc ggtcgtgacc gcgcagcagg
     5461 tgggcagcta caagccggac ccgcgcaact tcgcgcgcgc catcgagcgc gtcggcgtgt
     5521 cgcgcgcgcg cctcctgcac gtggcccaga gcctcttcca cgacatcgcc ccggccaaag
     5581 ccgccgggct ggcgaccgtc tgggtcaacc gccgccacga tcgtccgggc tttggcgcga
     5641 ccccaccggc gagcgccgaa ccggacctgg aagtgccgga cctggcgacg ctggcgcgtc
     5701 tggtggaagc cgcgccgagg ccgggagacc agtagacggt ggcgacgagg acggatcctt
     5761 cgactgcgct gcggatgaca tgaatgggga gaggagcgta cgagtttgtc acgtcgtcgc
     5821 cgtcagcccc tcaccaaccc tgtcatcctg ggcgcagtcg aaggatccgc accccacgcg
     5881 ccacccaatg ttgccgaagg agaagacgca gccacatgga caagatggac aagcgtacgg
     5941 gccatgacag cgccggctgg gatctcgata cccggctgat ccacggctcc caggagccgg
     6001 acccggccac tggcgcgctg gtgccgccga tccacccggc gtcgacgttc gtgcgccgcc
     6061 cgatcgacga cgacgccacc taccgctacg cgcgcggcga caacccgacg cgcgcggcgc
     6121 tggagacgat tctggccgaa ctggagcacg gcgtggccgc ctcggccttc ggctccggca
     6181 tggcggccgt caccgccgcc ctgcagctcc tctctggcgg cgaccacgcc atcgtcggct
     6241 acgactgcta cctgacgacc tacgacttct ttgccaacga cctgccgcgt tacggcatta
     6301 gcgccgattt cgtcgatctg
//
LOCUS       DASUBI010000440         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_44019, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000440 DASUBI010000000
VERSION     DASUBI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44019"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1349)
                     /locus_tag="VFU72_08445"
     CDS             complement(324..1349)
                     /locus_tag="VFU72_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU5423553.1"
                     /translation="MHRRWLKAITILVVVAAAVALLGILFPIVILLAIALVALAFWKP
                     RWIARLARLRGPRRLPAWLTRSPVVFALVAALSLVLVSSAAGIVVYARWSPLARLIHD
                     RMESPAPVGAGGLTPARVTGVVDGDTLKVTFDGRGQTVRIIGVDTPETKDPRQPVMCY
                     GAEATAFARQLVDQAGGRVLLEKDVSETDQYGRLLRYVWLELPGGRRMLNEELVTQGY
                     AQVSTYPPDVRYQQVFIAEERAARQADLGLWGACGSFGVPVTPTSSATRTTTPRPSPG
                     GAIDHAGVPPLSAFECPPDYPIKGNADSGIYHQPGDPAYLRTKPEICFATPADAHAAG
                     FRGAGGS"
BASE COUNT          381 a          707 c          726 g          337 t
ORIGIN      
        1 cgctcagggc gtccatgaat aatccgggct agagaggtgg tataggggtg gtattgctgc
       61 gccgcgagta atgggaggtc aggctcgcga cgcgcgggaa ggggttgggt ctatctccag
      121 gttagccgct tcccgtaaca cgcacatcac aggactcccg cgtgcgtcac aaaatcgtga
      181 cacggcgaat ccgtggcggc gcttcgagct tgtgctctgt cgtgggaacg ctgattcagg
      241 tgacgccgct ggtgggccct tccacaccaa ggcttggatt tccgtgccga gggcaatcgt
      301 cggccgcaag cttccccgag gactcatgac ccgccggcgc cgcggaaccc cgccgcgtgg
      361 gcatccgccg gagtggcgaa gcagatctcg ggtttggtgc gaaggtaggc tgggtcgccc
      421 ggctggtggt agatgccgga gtcggcattg cccttgattg ggtagtcagg cgggcactcg
      481 aacgccgaga gcggcggcac ccccgcgtga tcgatggccc cgcccggcga cggccgcggc
      541 gttgtcgtcc gggtggcgga cgaggtcggt gtgaccggca cgccaaagct gccgcaggcg
      601 ccccaaagac caagatccgc ctggcgcgcg gcgcgctcct cggcgatgaa gacctgttga
      661 taccgtacgt ccggcggata ggtgctgacc tgcgcgtagc cctgcgtgac cagttcctcg
      721 ttgagcatgc gccggccgcc cggcaactcc agccagacgt agcgcagcag tctgccgtac
      781 tggtccgtct cgctgacatc tttttccaaa aggacccggc caccggcctg atcgacgagt
      841 tggcgcgcga atgcggtcgc ctcggcccca tagcacatca ccggttgacg cgggtctttg
      901 gtctccggcg tatcgacccc gatgatgcgc accgtctgcc cccgcccatc gaacgtcacc
      961 ttgagcgtgt cgccgtccac gacgccggtt acccgagctg gcgtcaggcc gcccgcgccg
     1021 accggcgcgg gcgattccat cctgtcgtgg atcaaccgcg ccagcgggct ccagcgggcg
     1081 tagacgacga tgcccgcggc ggacgagacg agcaccagcg acagggcagc cacgagcgcg
     1141 aagacgaccg gcgaacgggt cagccaggcg ggcaggcgcc gcggcccgcg cagccgggcg
     1201 agccgggcga tccagcgcgg cttccagaac gccagcgcta ccagggcaat ggcgaggagg
     1261 atgacgatgg ggaagaggat gccgagcaac gccacggcgg cggcgacaac cacgagaatg
     1321 gtgatggctt tcaaccagcg acggtgcata cgctcctcac gtgaaccggg ccgcgtcggc
     1381 cgccgccgag ccgttcgcgc gccgaacttc gaccaaaacc gccacgcgat cggtctatca
     1441 gtgcccgctg tgtgtcatga tgcagtgatg cccgcgccac tggcaccacg agagaacatg
     1501 gcggaatcgt aactgcgtag caccgattag agcaccggcc accgaacacc cggcaactgg
     1561 accacatttg gagagatcct tcgcggtggc tcagagcctg ccctgagccg ccgcgaagga
     1621 tctctcctca gcctcggctg gtagcgaccg tcaggccaac gccgtctcgc cgtcccatac
     1681 cgctacgtgg ccggcgctct aggtttgaag gaacacgcgc gtgcgggagt gagacagaga
     1741 acagcggggg tcaaatcggc ctggacccac gagggtgtgt ggtctggaac ggcgaccggc
     1801 acccgatgtg gcgcggtgaa cgtgatatgg gcactagtaa ctcactacag gcgccagctc
     1861 gcgcgacacg ggacggagag accgcgtcgc gggtacgagc gccgccgatt ggatcgttgc
     1921 cgccttttca gggctgatgc cacgactatg gttggcgacc gctcgcagaa taccagtcga
     1981 cttccacggg gcggcgcgtc catctgatgg gacgcggcgt agcaccctgg caggaaccgg
     2041 cgtgctctga cgcctcgtcg ccctgccccg tggaagtcga cactcatccg gacacgtctg
     2101 gctcatcgat ctccgagtgg acccaatcgc gaatagattc cgcctcctct c
//
LOCUS       DASUBI010000441         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_546354, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000441 DASUBI010000000
VERSION     DASUBI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546354"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFU72_08450"
     CDS             complement(<1..529)
                     /locus_tag="VFU72_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423554.1"
                     /translation="MTDSDFSATSAATANGAQPDGAPTDTPSVGAPARTPLIAVRNLA
                     KTYLLGETAVPALRGVSLDVYPGEFVAVMGPSGSGKSTFMNLIGCLDRPTSGDYLLRG
                     LPVNTLSPDRLADIRNRRIGFVFQGFNLLARATALRNVELPMVYLGLSKDEQRRRAAR
                     ALRLVGLGNRLDHKPM"
     gene            complement(590..2023)
                     /locus_tag="VFU72_08455"
     CDS             complement(590..2023)
                     /locus_tag="VFU72_08455"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5423555.1"
                     /translation="MSMLDTPKGAGRAALDFPLFGDDDGAEEWTAGAPPLKTPRWRSR
                     PVLIAAAAVVVLALIITPLAIMRGRKTTITYASASVRQGTLAVTVTATGLLQSATYDV
                     NFATSGTIAEIDVSVGQKVKSGQVLAKLDATALRQALSLAQTQAGQAYDQEQNAIFSC
                     NNQTNPPPNCVQAAEAQYTIALDQLHTAQQNLANATLKAPHSGVIGVVNGHIGGTPGA
                     GSSASSGSGSSGAFIEILDLSSLQVVADASEADIGKLALGQPASFTVSAYGSRQFAGT
                     VGTISPLGQTTSNVVTYPVTVNVDTSSLNGASLLPGMTANVTITTARRQGVLLVPASA
                     ITFARTELGNGAISRAQVAPALRQAVQMLQQTQNSDPTASQDNLSASFVLERSNTQWV
                     VKPVVLGLTNGNVYEVLAGLTAGETMITGQQGATTTTGSGATGGGFGGGFGGGFGGGR
                     GGAGGGGRGGSGGGTGGSGSGGSGTGG"
BASE COUNT          320 a          696 c          690 g          360 t
ORIGIN      
        1 gcatcggctt gtgatcgagc cggttgccca gccctacgag ccgcagcgcc cgcgccgccc
       61 gccgccgctg ctcgtccttt gagagtccca ggtagaccat cggcagctcc acattgcgca
      121 gcgcggtcgc ccgcgccagc aaattgaagc cctggaacac gaagccgatg cggcgattac
      181 gaatatccgc caggcggtcg ggcgagagcg tgttgaccgg caaccccctg agcagatagt
      241 cgccgctggt tgggcggtcc aggcagccga tcagattcat gaatgtggac ttgcccgagc
      301 cggatggccc catcacagcg acgaattcac cagggtagac atccagcgag acgccacgca
      361 gcgcgggcac cgccgtctcg ccgagcaggt aggtcttggc cagattgcgg acggcaatca
      421 gcggtgtcct ggctggggca cccaccgacg gcgtgtcggt cggcgcgccg tcgggctggg
      481 cgccgttggc cgtcgcggca gacgtggcgg aaaagtcaga atcggtcacg gctcgcctcc
      541 tgggaagggt gctgctggtc gcgggtgtcg cgtgcatcgg aagcgccgtt catccgcctg
      601 tcccgctgcc accggaacca ctgccgccgg tgccgccgcc tgagccaccg cgacccccgc
      661 cgccagctcc gccacggccg ccaccgaacc cgccgccgaa cccgccgcca aagccgccgc
      721 cggtggcgcc agacccggtc gtcgtagtcg cgccctgctg gccggtgatc atcgtctcgc
      781 ccgccgtgag gccggccagc acttcgtaca cgttgccgtt ggtgaggccg agcacgaccg
      841 gcttgacgac ccactgagtg ttcgagcgct ccagcacgaa gctcgccgag agattatctt
      901 ggctggccgt tgggtcgcta ttctgggttt gctggagcat ctggacggcc tgtcgcagcg
      961 ccggggccac ctgcgcgcgg ctgattgcgc cgttgcccag ctcggtccgc gcgaaggtga
     1021 tcgcgctcgc cggaaccagg agcacaccct ggcgacgggc cgtcgtgatc gtcacgttgg
     1081 ccgtcatccc cggcagcagg cttgccccgt tcaggctgct cgtatccaca ttgaccgtta
     1141 ccggataggt cacgacgttc gaggtggtct ggcccaaggg tgagatggtg ccgacggtgc
     1201 ccgcgaactg gcggctgcca taggcgctga cggtgaagct ggcaggctgg ccgagcgcga
     1261 gcttgccgat atcggcctca ctggcgtctg cgacgacctg cagcgacgac agatcgagga
     1321 tctcgatgaa ggcgccgctt gatcctgagc cactgctggc ggagctaccc gcgccgggtg
     1381 tgccaccgat atggccattg acgacgccga tcacccctga gtgcggcgct tttaacgtcg
     1441 cgttcgctag attctgctgg gccgtgtgga gttggtcaag cgcgatagtg tactgcgctt
     1501 cagcggcctg cacgcagttg ggtggcggat ttgtctggtt gttacaggag aagattgcgt
     1561 tctgctcttg atcgtaggcc tgtcccgcct gggtttgcgc gagcgacagc gcctgccgca
     1621 gcgcggtggc gtcgagcttc gccagcacct gtcccgactt caccttctga ccgacagaga
     1681 cgtcaatctc ggcgatcgtg ccgctcgtgg cgaagttgac gtcgtaggtc gcgctttgca
     1741 gcaggccggt ggcggtgaca gtgacggcaa gcgtaccctg ccgcacgctc gcagaggcat
     1801 acgtgatggt tgtcttgcgg ccgcgcatga tggcgagagg cgtgatgatc agcgccagca
     1861 cgaccaccgc cgcggcagcg atcagcaccg gtcggctgcg ccagcgcggt gtcttgagtg
     1921 gtggtgcccc tgcggtccac tcctcagcgc cgtcatcgtc tccgaaaagc gggaagtcga
     1981 gcgcggcccg cccggctccc tttggcgtat ccagcatgct cataccacat cccccacgca
     2041 cgcctcaagg acgtgctcgc ccatac
//
LOCUS       DASUBI010000442         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_83723, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000442 DASUBI010000000
VERSION     DASUBI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83723"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFU72_08460"
     CDS             complement(<1..249)
                     /locus_tag="VFU72_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5423556.1"
                     /translation="MRSPVKIAKRQLTLSTHHIHTLPVLVLMAHSRCNCRCVMCDIWR
                     ANRHGQEIAPELLARHVEELRRLGVEWVILSGGEALMHS"
     gene            complement(328..1803)
                     /locus_tag="VFU72_08465"
     CDS             complement(328..1803)
                     /locus_tag="VFU72_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5423557.1"
                     /translation="MIILYNPTSNATHKPVLPMSLLALGAQLEGRHDYRIVDGNLARD
                     PLATLDTAIRESGADLLGITVMPGPQLSQAVPFSRELKARHPELEIVWGGYFPTQHYE
                     VALRADYVDYVVRGHGELVFRAFLDARAAGEDPLATPGLAYRAPGTGEIVSNAMAPIP
                     HPDRMPEFPYQRLEMARYVRPTFLGRRTLPHHSSYGCPFFCNFCAVVNMVGGRWFAQS
                     AERTAGVARQMVEQWGVDAVEFYDNNFFVHQARTAEFAERVMDLGIAWWGEGRIDTML
                     KYSERTWELMRDSGLKMVFLGAESGSDETLKRMNKGGTASTEKTLAIARKMADYGIVP
                     EFSFVLGNPPDPEGDVRQTIEFIRKLKGVNPRAEIILYMYTPVPLAGELYDQAKAEGF
                     QFPETLESWISQDWQEFAQRRSSHMPWLDDPLRRRVHNFERVLNAYYPTVTDARLHGP
                     WRGLLKSLSAWRYHLGVYDHPLELRVLQKALAYQRPETSGF"
     gene            complement(1814..3676)
                     /locus_tag="VFU72_08470"
     CDS             complement(1814..3676)
                     /locus_tag="VFU72_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5423558.1"
                     /translation="MDVITARQQAQATLPPAPRFVCPVCQGALESVGEDRLRCARDRL
                     DFERVDGIWRFLPPDRLEHYAQFVREYTTVRAREGRGGLNAAYYRALPFADRSGRFAA
                     DWRIRGQSFTTLIDEVLAPLEETAARPLVVLDLGAGNGWLSHRLAQRGHVVLALDLQT
                     NAADGLGAFVHYAHRFTPVQAEFDRLPLADAQADLVIFNGSLHYAADIERTLVEALRV
                     LRPDGRLVIMDTPVYHDPSSGQAMLQEREEQFARQYGFPSNAVPMEGFLTFQRLDALA
                     DALGLRWWLIEPDYGLGWALRPWRARLRGRREPARFVVAVGRREEAFSEGEDLPVAGS
                     HLARGAKAAALNLTKPTEGARFGRRPQIARGFEGLQRGAWRRVLEVRLRWVRGRQRRV
                     VLEQVEGLDLVVLPGVFNPKLLRSGAFFADVLSGLVPEGAAVLDLGTGSGVAALAAAR
                     RAGRVVASDVNPEAVRCARINALLNGAAERVEVRQGDLFVPVAGERFDLILFNPPYYR
                     GEPRDLADVAWRSPDVAERFAGGLSGHLTPNGAALVILSSDGEPAVFLGACEAAGLSI
                     DVVGRRDLINEVFTIYRIAPQTTKGGLQTRPYDHSNILPSPRGEGRGVRAPRWG"
     gene            complement(3687..4496)
                     /locus_tag="VFU72_08475"
     CDS             complement(3687..4496)
                     /locus_tag="VFU72_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5423559.1"
                     /translation="MAETVAQPHAFDGVAGRYDADFSRQQLGQWLRAAVWRVLGESFA
                     PGQRALDLGCGTGEDALWLARRGVSVTALDASTGMLAVAREKAAGAALSDGISFAQAD
                     LNDLRAAGLPAGGFDGAYADFGPLNCVADRAALGNELARLIRPRGRLVTVVMGPLCPW
                     EVAWYLAHGHARTASRRFRRRVRSRVGDGEITVSYPSPRRLRAELAPGFQQVALLGLS
                     TLLPPSDLGHLIPKAPRLFAALNAADERLRGRFPFTWFNDHYIAVFERAWR"
BASE COUNT          746 a         1662 c         1554 g          640 t
ORIGIN      
        1 cgagtgcatc agcgcctcgc cgcccgagag gatgacccac tcgacgccca gtctgcgcag
       61 ctcctcgacg tggcgcgcca gcagctcggg cgcgatctcc tgaccgtggc gattggcgcg
      121 ccagatgtcg cacatcacgc agcggcagtt gcagcggctg tgcgccatca gcaccagcac
      181 cggcaaggtg tggatatggt gcgtgctgag cgtcaactgc cgcttggcga tcttcaccgg
      241 cgagcgcatc gcgaacctcg attccgtccc ggttcgatta ttcgtacccc ctctcccgcg
      301 cacgggagag ggcagggtga gggcctgtca gaatccgctc gtctccggcc gctggtaggc
      361 cagcgccttc tgcagcacgc gcagctccag cgggtgatcg tagaccccca ggtggtagcg
      421 ccaggcgctc agcgacttca gcaggccgcg ccagggaccg tgcagtcgcg cgtcggtcac
      481 cgtcggatag taggcgttga gcacgcgctc gaagttgtgg actcgacgcc gcagcgggtc
      541 gtcgagccag ggcatgtggg agctgcgccg ctgcgcgaac tcctgccagt cctggctgat
      601 ccaactctcc agcgtctccg ggaactgaaa cccctcggcc ttggcctggt cgtagagttc
      661 gccggccagc ggcaccggcg tgtacatgta gaggatgatc tcggctcgcg ggttgacccc
      721 tttgagcttg cgaataaact cgatcgtctg acgtacgtcg ccctcagggt cgggtgggtt
      781 gccgagcacg aaggaaaact ccggcacgat gccgtagtcg gccatcttgc gcgcgatcgc
      841 cagcgtcttt tcggtcgagg cggtgccgcc cttgttcatg cgcttgagcg tttcgtcgga
      901 gccggactcg gcccccagaa agaccatctt caggccggag tcgcgcatca gctcccaggt
      961 gcgctcgctg tacttcagca tggtgtcgat gcgcccctcg ccccaccagg cgatgccgag
     1021 gtccatgacg cgctcggcga actcggccgt gcgggcctgg tgcacgaaga agttgttgtc
     1081 gtagaactcg accgcgtcga cgccccactg ctcgaccatc tggcgcgcca cgccggcggt
     1141 gcgctcggcc gattgcgcga accagcgccc cccgaccatg ttgaccaccg cgcagaagtt
     1201 gcagaagaaa gggcagccat agctggagtg gtgcggcagc gtgcgccgcc ccaggaaggt
     1261 gggccggacg tagcgcgcca tctccagccg ctggtagggg aactccggca tgcggtcggg
     1321 gtgtgggatc ggcgccatcg cgttgctgac gatctcgccc gtgcccggcg cacgatacgc
     1381 cagccccggc gtcgccagcg gatcctcgcc ggccgcgcgt gcgtcgagaa aggcgcggaa
     1441 gaccagctcg ccgtgaccgc gcaccacgta atcgacataa tcggcgcgta gtgcgacctc
     1501 gtagtgctgg gtggggaagt agccgcccca gacgatctca agctctgggt gccgcgcctt
     1561 cagctcgcgc gagaacggca cggcctgact gagctgcggg ccgggcatga cggtgatccc
     1621 gagcaggtcc gcgccgctct cgcggatggc cgtgtccagt gtggccagcg gatcgcgcgc
     1681 caggttgccg tcgacgatgc ggtagtcgtg ccgcccctcg agctgcgcgc cgagcgccag
     1741 cagcgacatc ggcagcaccg gcttgtgggt ggcgttactc gtcgggttgt agagaatgat
     1801 catggacagg ccctcacccc cagcggggag ccctcacccc ccgcccctct ccgcgcggag
     1861 aggggagaat attcgaatga tcgtaggggc gggtttgcaa cccgcccttc gtggtctgcg
     1921 gcgcgatgcg gtagatggtg aagacctcat tgatcaggtc gcgccggccg acgacatcga
     1981 tagacaggcc ggcagcctcg caggcgccga ggaagacagc aggttcgccg tcgctggaga
     2041 ggatgaccaa cgccgcgccg ttgggcgtga gatggcccga caacccgccc gcgaagcgct
     2101 cggcgacgtc cggcgagcgc caggcgacat cggccagatc gcgcggctcc ccgcggtagt
     2161 aaggcgggtt gaacagaatc aggtcgaagc gctcgcccgc caccggcacg aagaggtcac
     2221 cctggcgcac ctcgacccgc tcggcagcgc cgttcagcag cgcgttgatc cgcgcgcagc
     2281 gcaccgcctc cggattgacg tcgctggcca ccacccgccc ggcccgccgc gcggcagcga
     2341 gcgccgcgac cccggagccg gtccccagat cgagcaccgc cgcgccctcc ggcaccaggc
     2401 cgctcagaac gtcggcgaag aacgcacccg agcgcaggag cttcgggttg aacacccccg
     2461 gcagtaccac cagatcaagt ccctcgacct gctccagcac cacgcgcctc tgccgcccgc
     2521 gcacccagcg caggcgcacc tccaacacgc gacgccaggc cccacgctgt agcccctcga
     2581 agccacgcgc gatctggggc cgccgcccaa acctagcccc ttcagtgggc ttcgtcaggt
     2641 ttagcgcggc ggctttagcc ccgcgcgcca ggtgactccc ggcaacaggg agatcctcac
     2701 cctccgaaaa cgcttcctcg cgccgcccga ccgccaccac aaaccgcgcc ggctcgcgac
     2761 gcccgcgcag ccgcgcccgc cagggacgca gcgcccaacc cagaccatag tcaggctcga
     2821 tgagccacca gcgcagcccg agcgcgtcag ccagcgcgtc cagccgctgg aaggtcagga
     2881 agccctccat cggcaccgcg ttggatggga agccgtactg ccgcgcgaac tgctcctcac
     2941 gctcttgcaa catcgcctgg ccgctggagg ggtcatggta gaccggcgtg tccatgatca
     3001 ccaggcggcc gtccggacgc aggacgcgca gcgcctcgac caacgtccgc tcgatatcgg
     3061 ccgcgtagtg cagcgagccg ttgaagatca ccaggtccgc ctgggcgtcg gcgagtggca
     3121 ggcggtcgaa ctcggcctgc accggggtga agcggtgcgc gtaatggacg aacgctccaa
     3181 gcccgtcggc ggcgttggtc tgcaggtcga gcgcgagcac gacgtggccg cgctgggcca
     3241 gccggtggga gagccagccg ttgcccgcgc cgaggtcgag caccaccagc ggacgcgcgg
     3301 ccgtctcctc cagcggagcc agtacctcat cgatcaacgt tgtgaagctc tgcccgcgaa
     3361 tgcgccagtc ggcggcgaag cgcccgctgc gatcggcgaa ggggagcgcg cggtaatagg
     3421 ccgcgttcag gccgccgcgg ccctcgcgcg cccgcaccgt cgtgtactcc cgtacgaact
     3481 gcgcgtagtg ctccagccgg tcgggaggaa gaaagcgcca gatgccgtcg acacgctcga
     3541 agtcgagccg atcgcgcgcg cagcgcagac gatcctcgcc caccgactcc agcgctccct
     3601 gacagaccgg gcagacgaag cgcggagccg gcggcaacgt ggcctgtgcc tgctgcctgg
     3661 ctgtgatcac gtccaccgtc acctcgctac ctccacgctc gctcgaagac tgcgatgtag
     3721 tggtcgttga accaggtgaa ggggaagcgc ccgcgcaggc gctcgtcggc ggcgttgagc
     3781 gcggcgaaga ggcgcggcgc cttcgggatc aagtggccga ggtcggaggg cggcagcagc
     3841 gtgctcagtc cgaggagtgc gacctgctgg aagcccggcg ccagctcggc gcgcaaccgc
     3901 cggggcgagg gatagctcac cgtgatctcc ccgtcgccga cccgcgagcg tacccggcgg
     3961 cggaagcgcc gcgaggccgt gcgcgcgtgg ccatgcgcga ggtaccaggc gacctcccag
     4021 gggcagagcg gccccatcac caccgtcacc agccgtccac gggggcggat cagccgcgcc
     4081 aactcgttcc ccagggccgc gcgatcggcc acgcagttga gcggaccgaa atcggcatag
     4141 gcgccgtcga agccgcccgc gggcaagccc gccgcgcgga gatcgttcag gtccgcctgg
     4201 gcgaagctga ttccatccga caacgccgcg cccgccgctt tctcgcgcgc aaccgccagc
     4261 atccccgtcg aggcatccag cgccgtgacg ctcacaccgc gacgggccag ccagagggcg
     4321 tcctcaccgg tgccgcagcc caggtcgagc gcccgctgac ccggcgcgaa ggactcgccc
     4381 agcacgcgcc agacggccgc gcgcagccac tgcccgagct gctgccggct gaagtcggcg
     4441 tcgtagcggc ccgcgacacc gtcgaacgcg tgcggctggg cgacggtctc agccacgctc
     4501 gacctcgcgc tcggtgacgc gcatcccgac ccgcccgatc tgggcgttga ccagcgcctt
     4561 ggccatacgc cggtagcgcg gccgctcgct ctggcgctcc tg
//
LOCUS       DASUBI010000443         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1267404, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000443 DASUBI010000000
VERSION     DASUBI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267404"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..817)
                     /locus_tag="VFU72_08480"
     CDS             complement(71..817)
                     /locus_tag="VFU72_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5423560.1"
                     /translation="MSQELESVNAPGRVLVVMAHPDDAEFICGGTIAKWAAAGSHVVF
                     ALGTRGDKGSSDPGMSSERLMEIREHEQHDAARALGVKEVEFLGFRDAELVADLRLRE
                     AVTRVIRKHRPDALICQDPTARWSGQDYIQHPDHLAMGEATLAAVFPSARDPLTFPQL
                     LAEGLQPHKTPEVYLAAARESDIWIDISESFETKIAALNAHKSQMKDWDPGPEMRRWA
                     EDNAAAARFHRFPGAGEMRLAESFKYFRLD"
     gene            complement(957..>3836)
                     /locus_tag="VFU72_08485"
     CDS             complement(957..>3836)
                     /locus_tag="VFU72_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5423561.1"
                     /translation="KSTDGGKSWQNVGLAATRHISGIAVHPQNPDIVYVGALGHAWGP
                     NEERGVYRSTDGGKNWQKVLYKSPKAGVAQLSMDPNNPRVLFATLWQGQRYPYALHSG
                     GDDSGIWRSTDGGDTWQDITRNPGLPQKAMLGRMGISVSPARSGRVWAVIEADQGQGG
                     FYRSDDNGDTWQLMTDQSDLRRRPWYYQYVMADPVDPDTVYVCNLQFWKSSDGGKTFE
                     AIPTAHGDNHALWIDPQDNKRMIEGNDGGAVVTYNGGVSWSTLDNQPTAQFYHVAVDD
                     QVPYHIYGSQQDNYAMRLPSLGREGAISKNYWTEPGGGESGYIAISPKPPHQVFGGGI
                     GTGLGHGRLLAWNPETGQKRNVTVWPEVVTGLGSTTGANALKYRFQWTFPVEFSPHDP
                     DVLYVTSNHVHRSRDEGHSWEVISPDLTHNNPQWLEASGGPITADNSGAEIYCTIFAF
                     RESPSERGVLWAGSDDGLIHVSRDNGQSWQNVTPPQNLMPQWSQILIIEPCAHHNGEV
                     YVAATRYKFDDPSPFLFKTSDYGQTWTSINGNLPKECITRVVREDPEVEGLLYLGTET
                     GLYVSLDGGQSWQPFQANLPVTPIYDLAVKDGDLVAATHGRSFWILDDLSPVRELAGM
                     KGGFGEQTHLFTPGYAVRYRTYGRGYGRKRPDMVSYMATGPISIGYWDKQTPMGTSSP
                     DFLDAGKNPPNGAVVHYYLNQAPGEDEEITLTFLDHDGQEIRSYSSKAEEPPRVPAAQ
                     GMNRFLWDLRTQPPVKLDVDPIADPFQRMMQQNVAPRVLPGNYQVQLKAGGQTLTRAF
                     TVSVDPKLINMSPADLTAQHDLKRQIVGSINEINQALNQINKVRGQVAHWEDRVKGTS
                     QEQQLAEPAKQLKDKLSDIESRLIVANPGQPKSPPSQLRERLFSLASMIDESDDKPTQ
                     GAQEVYQVLSQQVQEQMQQLQQVMQQEVPQFSQKVQQANVAPVGP"
BASE COUNT          509 a         1254 c         1368 g          705 t
ORIGIN      
        1 attcccgtta ggggcagacc cccgtgtctg ccctcgcccg tgcggcgccg gaccttgtcg
       61 acagccgtcc tcagtccagt cgaaaatact tgaacgactc ggccagccgc atctcgccgg
      121 cgccggggaa gcggtggaag cgcgcggcgg ccgcgttgtc ctcggcccag cgccgcatct
      181 ccggccccgg atcccaatcc ttcatctggc tcttgtgcgc gttcagcgcc gcgatcttgg
      241 tctcgaatga ctcgctgatg tcgatccaga tatccgactc acgcgccgcg gccaggtaga
      301 cctcgggtgt cttgtgcggc tgcagcccct cggccagcag ctgcgggaag gtcagcgggt
      361 cgcgcgccga ggggaagacc gccgccagcg tggcctcgcc catcgccaga tggtccgggt
      421 gctggatgta gtcctgcccc gaccagcgcg cggtcgggtc ctgacagatc agcgcgtcgg
      481 gccggtgctt gcgaatgacg cgggtgacag cctcgcgaag gcgcagatcg gccaccagct
      541 cggcgtcgcg gaagccgaga aactcgacct ctttgacccc gagggcgcgc gcggcgtcgt
      601 gctgctcgtg ctcgcggatc tccatcagcc gctcgctcga catgccgggg tcgctgctcc
      661 ccttgtcgcc gcgcgtgccg agcgcgaaga cgacgtggct gccggcggcg gcccacttgg
      721 cgatggtgcc gccgcagatg aactcggcgt cgtccggatg cgccatcacc accagcacgc
      781 gccccggcgc gttgacgctc tctaactcct ggctcatgct cctccttacg cgccctcacc
      841 cccgcccctc tcccaatact gggcgagggg agcacgcaag cgtcgcccgt gtttgactcc
      901 ccttctccca gcattgggct ccgttcgaga aggggcgggg gatgagggcc cgtccgctac
      961 ggcccgaccg gcgccacgtt cgcctgctgg accttctggc tgaactgcgg cacctcctgc
     1021 tgcatcacct gctgcagctg ctgcatttgc tcctgcacct gctgcgacag gacctgatag
     1081 acctcctggg cgccctgggt cggcttgtcg tccgactcgt cgatcatcga ggccagcgag
     1141 aagagacgct cgcgaagctg gctgggcggc gacttcggct ggcccgggtt ggcgacgatc
     1201 aggcggctct cgatatcgct cagcttgtcc ttgagctgct tggccggctc ggcgagctgc
     1261 tgctcctggc tggtgccctt gacgcggtcc tcccagtgcg ccacctggcc gcgcaccttg
     1321 ttgatctggt tgagcgcctg gttgatctcg ttgatcgacc ccacgatctg gcgcttgagg
     1381 tcgtgctggg ccgtcaggtc ggccgggctc atgttgatca gcttggggtc gaccgagacc
     1441 gtgaaggcgc gcgtcagcgt ctggccgccg gccttgagct gcacctggta gttgccgggc
     1501 agcacgcgcg gggcgacgtt ctgctgcatc atgcgctgga agggatcggc gatcgggtcg
     1561 acgtcgagct tgaccggcgg ctgggtgcgc aggtcccaga ggaagcggtt catcccctgg
     1621 gcggccggca cgcgcggcgg ctcctcggcc ttgctggagt agctacgaat ctcctggccg
     1681 tcgtggtcga ggaaggtcag cgtgatctcc tcgtcctcgc ccggcgcctg gttcaggtag
     1741 tagtggacga ccgcgccgtt gggcgggttc ttgccggcgt cgaggaaatc gggcgagctg
     1801 gtgcccatcg gggtctgctt gtcccagtac ccgatcgaga tcgggccggt ggccatgtag
     1861 ctgaccatgt ccggccgctt gcgcccgtag ccacgcccgt aggtgcggta gcggacggcg
     1921 tagcccggcg tgaagaggtg cgtctgctcg ccgaagccgc ccttcatgcc ggccagctcg
     1981 cgcaccggcg agaggtcgtc gaggatccag aaggagcggc cgtgggtggc agcgaccaga
     2041 tcgccgtcct tgacggccag atcgtagatc ggcgtgaccg gcaggttggc ctggaacggc
     2101 tgccagctct gcccgccatc cagggagacg tagaggccgg tctcggtgcc gagatagagg
     2161 agcccctcga cctccgggtc ctcgcgcacc acacgggtga tgcactcctt gggcagattg
     2221 ccgttgatcg aggtccaggt ctgcccgtag tcgctcgtct tgaagaggaa cgggctcgga
     2281 tcgtcgaact tgtagcgcgt ggccgccacg tagacctcgc cgttgtggtg cgcgcagggc
     2341 tcgatgatca ggatctgcga ccactgcggc atcaggttct gcggcggggt gacgttctgc
     2401 cagctctggc cgttgtcgcg cgaaacgtgg atcagcccgt cgtcggagcc ggcccagagc
     2461 acgccgcgct ccgagggcga ctcgcggaag gcgaagatgg tgcagtagat ctcggcgccg
     2521 gagttgtcgg ccgtgatcgg gccgccggag gcctccagcc actgcgggtt attgtgggtg
     2581 agatccggcg agatgacctc ccagctgtgc ccctcgtcgc gcgagcgatg cacgtggttg
     2641 gaggtgacgt agagcacgtc ggggtcgtgc ggcgagaact cgaccgggaa cgtccactgg
     2701 aagcggtact tgagcgcgtt ggcgccggtg gtcgagccca ggccggtgac gacctccggc
     2761 cagacggtca cgttgcgctt ctggccggtc tcggggttcc aggccagcag gcgtccgtgg
     2821 cccaggccgg taccgatgcc gccgccgaag acctggtgcg gtggcttggg gctgatggcg
     2881 atgtagccgc tctcgccgcc gcccggctcg gtccagtagt tcttgctgat ggcgccctcg
     2941 cggcccaggc tgggcaggcg catggcgtag ttgtcctgct gcgagccgta gatgtggtag
     3001 ggcacctgat cgtcgacggc gacgtgatag aactgcgcgg tcggctgatt atcgagcgtg
     3061 gaccagctca cgccgccgtt gtaggtgacg acggcgccgc cgtcgttgcc ctcgatcatg
     3121 cgcttgttgt cctgcgggtc gatccagagg gcgtggttgt cgccgtgcgc ggtcgggatc
     3181 gcctcgaagg tcttgccgcc gtcgctcgac ttccagaatt ggaggttgca gacgtagacc
     3241 gtatcggggt ccaccgggtc ggccatgacg tactggtagt accagggccg gcgccgcagg
     3301 tcggattggt cggtcatgag ctgccaggta tcgccgttgt cgtcggagcg gtagaagcca
     3361 ccctggccct ggtcggcttc gatgacggcc cagacgcgcc cggaccgggc cggcgagacg
     3421 ctgatgccca tgcgcccgag catggccttc tgcggcagcc ccgggttgcg ggtgatgtcc
     3481 tgccaggtgt cgccgccgtc ggtcgagcgc cagatgccgg agtcgtcgcc gccgctgtgc
     3541 agcgcgtagg ggtagcgctg gccctgccag agcgtggcga agaggacgcg cgggttgttg
     3601 gggtccatcg acagttgcgc gacgccggcc ttggggctct tgtagagcac cttctgccag
     3661 ttcttgccgc cgtcggtgga gcggtagacg ccgcgctcct cgttggggcc ccaggcgtgc
     3721 cccagcgcgc cgacgtagac gatgtcgggg ttctgggggt ggacggcgat gccgctgatg
     3781 tggcgcgtcg ccgctagccc gacgttctgc cagctcttgc cgccgtcggt cgactt
//
LOCUS       DASUBI010000444         8000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_350124, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000444 DASUBI010000000
VERSION     DASUBI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350124"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFU72_08490"
     CDS             complement(<1..609)
                     /locus_tag="VFU72_08490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEU5423562.1"
                     /translation="MQTAFPVASGLTSEEKARLWAAADKLRGHLDAAEYKHVVLGLIF
                     LKYISDAFEEHYAELAAQRDEGIDPEDKDEYLAEGIFWVPRDARWSVLRDNAKQPTIG
                     TLVDGAMESIERENPALREVLPKGYGRPTLDKTRLGELIDLFTNLDVGGKAHRSRDVL
                     GQVYEYFLSEFASAEGKQGGEFYTPSPVVRVLVEMLAPFEGRV"
     gene            complement(715..1026)
                     /locus_tag="VFU72_08495"
     CDS             complement(715..1026)
                     /locus_tag="VFU72_08495"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5423563.1"
                     /translation="MLALVEQNLDAIRALCREYGVLRLELFGSAATGAFDPERSDLDF
                     LVEYPPDYDFGLWLTRYFEFKDRLEALLGHPVDLVMTGAVRKPHFRASVNQSRRLLYA
                     A"
     gene            complement(1057..1275)
                     /locus_tag="VFU72_08500"
     CDS             complement(1057..1275)
                     /locus_tag="VFU72_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HEU5423564.1"
                     /translation="MICKRGETHAGTTTVTLTRGSMTLVFRGVPAQVCDTCGEEYVDE
                     ATTAHLLAVAEEAAAAGVQVEVREYVAA"
     gene            complement(1565..2494)
                     /locus_tag="VFU72_08505"
     CDS             complement(1565..2494)
                     /locus_tag="VFU72_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5423565.1"
                     /translation="MDGRVIPRPGVSTAMKQMRWPMTAGTPNMHASSTGQALTGAAWL
                     DDHFEANRPEYEAQLRAVGIQPGWRVLDAACGSGSFLPLLAALVGPTGRLAALDLAPD
                     NVTVVEQRVAASALPCPVEARAGTVLELPYADDTFDAVWFANTSQYLTDAELATALAE
                     FRRVVRPGGLVAVKDADGTFLTILPAPLGSVLRLTEEAARGGRVQAAGVLRAPLLGGW
                     LRRAGLVGVWRRTTMIERSPPFDAPTGRFWRGSLAFYAAAAAEMTLPAADQEAWERLR
                     DPDALDRFLADADTCLFDANVLAVGRVPTAENG"
     gene            3117..5915
                     /locus_tag="VFU72_08510"
     CDS             3117..5915
                     /locus_tag="VFU72_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423566.1"
                     /translation="MAQEAARPQTSNGSSPNGQTEDDEIDEGLSEIDRLDPERRRLIA
                     ARDRHEPWSLWGPYLSERQWGTVREDYSANGTAWAYFPHDHARSRAYRWGEDGLLGIS
                     DDHGLLCFGLALWNEADPILKERLFGLTGPEGNHGEDVKEYYFYLDNTPTHSYMKALY
                     RYPQRAFPYAQLVEVNRRRTRQDPEFELFDTGVLAENRFWDITVEYAKATPYDLLIRL
                     TCTNQGPDPARLHLLPSLWFRNTWRWGLHERYAGLALAEPLAGEQGMVWIQATHYKLG
                     SYWLACEAQPECLFTENESNSERLWGTPNATPYVKDGIHAFVVEGQREAVNPEQHGTK
                     AAAYYQLDLGPGASQTLLLRLSHERGAEPFAGAEALIAQRRAEADAFYAGLRPPEMDD
                     DSFLVMRQAFAGLLWSKQHYRYDVARWLQGDPAMPPPPPERKHGRNHDWQTLNNNDVL
                     SIPDTWEYPWYASWDLAFHTVPLAMIDPELAKRQLILLLREWYMHPNGQLPAYEWAFS
                     DVNPPVHAWAAYRVYKIEERLTGRADRLFLERVFQKLLLNFNWWVNRKDVDGHNVFQG
                     GFLGLDNIGVFDRTVALPTGGHLDQADGTAWMGLFCLSMLAIALELARTNIAYEDMCI
                     KFFEHFLYIAGALNSIGEAVSIWNEDDGFYYDVLHLPDGTKTPLKVRSLVGVIPLFAV
                     ETIDPEGMEGLPQFASAFHWFLAHRPDLAALVSRWDVPGEGRRRMLALVHGHRMKRVL
                     TRVLDPQEFLSEYGVRSVSRYHRDHPYVLTLAGARYSVDYEPGESTSGVFGGNSNWRG
                     PIWFPLNFLLIESIQKYHHYYGDDFRIECPTGSGRFLTLWQVADELSCRLEWLFTRNA
                     SGRRPVFGDNELFQNDPRWRDYIPFHEYFHGETGAGLGASHQTGWTALVAKLLEQTSR
                     MRDLSADRQELTLAASRR"
     gene            5912..7906
                     /locus_tag="VFU72_08515"
     CDS             5912..7906
                     /locus_tag="VFU72_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5423567.1"
                     /translation="MSGATILDHPLVRRGRALCGELEPALGREWLVTNGLGGYASGTV
                     AGVNTRRYHGLLVAALNPPVERVMLVGGTVAWAGYGGRRYPLSTHEYADGTVDPNGFV
                     NIQSFHLEGALPVWTYALGDALLERRVWMAQGSNTTYLAWRLARGSLPLQLEVTPLLT
                     YRGFHELRRGQGWQPGCAPVAHGLRVEAGAGARPYWLLAPGAEYAPDGVWWWRFQHRA
                     EAARGLDAVDDLYAPGTFRLTLAPGQSAALICTTEEAPVLDFEAALTGERARQRALLQ
                     RAGVEADEPALQQLTLAADQFIVARPPGRSLIAGYPWFGDWGRDTMIALPGLTLATGR
                     PEEAAAILRTYAPFVRDGLLPNDFPNAPGAEVSYTSADAALWYLLAAYAYDRLAGDPS
                     LVDDLLPTLRGIVDCYAQGTRYGIGVDPADGLLRAGAPGLQLTWMDVKIGDWVVTPRA
                     GKPVELQALWYNALRILAGWLRARHDPAAAGYEAAADRAHTSFAARFVRPGLDHLADV
                     VDGPEGDDLACRPNQLFALSLPFALLEGDQAARVLESAGRQLLISYGLRSLAPADPHY
                     AGRYQGDPRQRDAVYHQGTAWAWLSGAYIDACVRVYGSPAPAAALLDPFTDQLCDAGL
                     GSISEIFDGDPPHHARGCIAQAWSVAEVLRCRRGLFWPTL"
BASE COUNT         1215 a         2942 c         2617 g         1226 t
ORIGIN      
        1 gacgcggcct tcgaagggcg ccagcatctc gaccaggacg cggacgaccg gcgagggcgt
       61 gtagaattcg ccgccctgct tgccctcggc gctggcgaac tccgagagga aatactcgta
      121 gacctgcccg agcacgtcgc gcgagcggtg ggccttgccg ccgacatcga ggttggtgaa
      181 gaggtcgatc agctcgccca ggcgcgtctt gtccaacgtc ggccggccgt accccttggg
      241 cagcacctcg cgcagcgcgg ggttctcgcg ctcgatcgac tccatcgccc cgtcgaccag
      301 cgtgccgatc gtcggctgct tggcgttgtc ccgcagcacc gaccagcgcg cgtcgcgcgg
      361 cacccagaag atgccctccg cgaggtactc gtccttgtct tccgggtcga tcccttcgtc
      421 gcgctgcgcc gcgagctcgg cgtagtgctc ctcgaaggcg tcggagatgt acttcaggaa
      481 gatgaggccg agcacgacgt gcttgtactc ggcggcgtcg aggtggccgc gcagcttgtc
      541 ggcggcggcc cagagtctgg ccttctcctc ggacgtgagg cccgaggcaa ccgggaaagc
      601 ggtctgcatg gcgtctcctc aggaaaagcg tcggttgcgg atattctatg acacttacga
      661 gtctgtgtga acagccagtt tggcttgctt gcggatgctc gcgatcacgg cgtatcaggc
      721 tgcatagagg agccgccggc tctggttcac gctcgcgcgg aaatgcggtt tgcgcacggc
      781 gccggtcatg acgaggtcga ccgggtggcc gagcagcgct tcgaggcgat ctttgaactc
      841 gaaatagcgg gtcagccaca gcccgaagtc atagtcgggc gggtactcga ccaggaagtc
      901 gaggtcgctg cgctcggggt cgaacgcgcc ggtggccgcc gagccgaaca gctccaaccg
      961 cagcacgcca tactcccggc agagcgcgcg aatcgcgtcc agattttgct cgaccagtgc
     1021 aagcatgacc tgacctttgg ccttgtttcc cgcctgtcag gcggcgacgt actcgcgcac
     1081 ctcgacctgc accccagctg ccgcggcctc ctccgccacc gctaggagat gagccgtcgt
     1141 ggcctcatcg acgtattcct cgccgcaggt atcacacacc tgagccggca cgccgcggaa
     1201 gacgagcgtc atgctgcccc gcgtcagcgt cacggtcgtc gtgcccgcgt gtgtctcgcc
     1261 gcgcttgcag atcacacact tgatgattga gccctctttc gaaacgtggc atcccaacgc
     1321 gctggatccg gttcgtagac cattatcacg atagtcgtat tcgcggccgc gttataggca
     1381 acgacgatat ggagcgcgcg ctggtcgatc cacccgagcg ccaggctact tggataaggc
     1441 tggtcagcgg ggtagcgctc aatcgtctcg tcattggtca gcaatcgtcg cacgccgcac
     1501 gatgcaatga atgccgcgtg acgcatggga gagtctcaac tctcgcgcgg tatcccgctc
     1561 tgcctcaacc attctccgcc gttggcaccc tgcccacggc caacacgttc gcatcgaata
     1621 ggcatgtgtc ggcgtccgcc aggaagcggt cgagcgcgtc tgggtcacgc agccgctccc
     1681 aagcctcctg gtctgccgca ggcagcgtca tctccgccgc ggccgccgcg taaaaggcca
     1741 ggctgccccg ccagaatcgt cccgtcggcg cgtcgaaagg cggggagcgc tcgatcatcg
     1801 tcgtgcgccg ccagaccccc accagccccg cgcggcgcag ccagccgccg agcagcgggg
     1861 cgcgcagcac gcccgccgcc tgcaccctgc cgccgcgcgc ggcctcttca gtgagccgta
     1921 ggaccgatcc gagcggcgcg ggcaggatgg tgaggaacgt gccgtcggcg tccttgaccg
     1981 cgacgaggcc gcccggccgc accacccggc ggaactccgc cagcgccgtc gccagctccg
     2041 cgtcggttag atactgcgag gtattggcga accagacggc gtcgaaggta tcgtccgcgt
     2101 acggcagctc gagcacagtg cccgcgcgcg cctccaccgg gcagggcagc gccgacgcgg
     2161 ccacccgctg ctccaccact gtgacgttgt cgggcgcgag gtcgagcgcg gcgaggcgac
     2221 cggtcgggcc gaccaacgcg gcgagcaagg ggaggaaaga gcccgagccg caggcggcgt
     2281 cgagcacgcg ccagccgggt tggatgccga cggcgcgcaa ctgcgcctcg tactccgggc
     2341 gattcgcctc gaagtggtca tcgagccatg cggcgcccgt gagcgcctgt cccgtcgagg
     2401 acgcgtgcat gttcggagtt cctgcggtca tcggccacct catctgcttc atcgccgtcg
     2461 acacgcccgg acgagggatg acgcggccat ccaagccgcc gattcacgga agatggccgg
     2521 atactaccgc gcatcgcgtg tgaccgcaaa ctcgttaccg atgtgaccgg aacaagcacc
     2581 ccatgtagcg aggaggcttg tccattgcgc gacgatcccg gtttacggcg cggcgttgcg
     2641 ctctccttgt tagcccgtca ccgtcccctc atcgcccggt cggggcacga gggacggtgc
     2701 caacacgtca tcctctcaag caatggggag taagtcgttc atgtcgaggg agcggcgtcc
     2761 cgctgagcca ctccactatt ctccagcaaa ccgaatgtgt cacgccgcat acagtacctg
     2821 ccggctcgcg gccacgctct ccgccaatgc gcgctcgcgc acggcaccaa ccatgaccag
     2881 gtcgactggt cgtccgagga gagcggcgag gcgatcgcgg acgtggggta tcgcggcgtc
     2941 cgtcacagcc cgaggttgta caagacgccg ttcccaacat gggaccatca tcctgcgcgg
     3001 ggacttgggc gtagactaga tggggcgggg atacttggcc ggggtacgag cggtggtcac
     3061 ggtggcaggg cgcgcttcgg gtgcggcccg aaccgggctg aaaggatgtc gcgaggatgg
     3121 cgcaggaggc ggcgcggccg cagacgagca atggttcatc cccgaacgga cagactgagg
     3181 acgacgagat cgacgagggg ctcagcgaga tcgaccggct cgatcccgag cgccgccggc
     3241 tgatcgccgc ccgcgatcgc cacgagccct ggtcgctctg ggggccctac ctctccgagc
     3301 gccagtgggg caccgtgcgc gaggactact cggccaacgg caccgcctgg gcctacttcc
     3361 cccacgacca cgcccgctcg cgcgcctacc gctggggcga ggacggcctg ctcggcatct
     3421 ccgacgacca cggcctcctc tgcttcggcc tcgccctctg gaacgaagcg gaccccatcc
     3481 tcaaggagcg cctcttcggc ctcaccgggc ccgagggcaa ccacggcgag gacgtcaagg
     3541 agtactactt ctacctcgac aacaccccga cccattccta tatgaaggcg ctctaccgct
     3601 acccgcagcg cgccttcccc tacgcgcaac tggtcgaggt gaaccgccgc cgcacgcgcc
     3661 aggaccccga gttcgagctg ttcgataccg gcgtgctggc cgagaaccgc ttctgggaca
     3721 tcaccgtcga gtacgccaag gccaccccct acgacctgct gatccgcctc acctgcacca
     3781 accaggggcc ggacccggcc cggctccatc tgctgcccag cctctggttc cgcaacacct
     3841 ggcgctgggg gctgcacgag cgctacgccg ggctggccct ggccgagccg ctggccggcg
     3901 agcaggggat ggtctggatc caggcgacgc actacaagct gggcagctac tggctcgcct
     3961 gcgaagctca gccggagtgc ctcttcaccg agaacgagag caacagcgag cgcctctggg
     4021 gcacgcccaa cgccaccccc tacgtcaaag acggcatcca cgccttcgtg gtcgagggcc
     4081 agcgcgaggc ggtcaacccc gagcagcacg gcaccaaggc cgccgcctac taccagctcg
     4141 atctgggtcc cggcgcctcc cagaccctgc tgctgcgcct cagccacgag cgcggcgcgg
     4201 agcccttcgc cggagccgag gcgctcatcg cgcagcgccg cgccgaggcc gacgccttct
     4261 acgccgggct gcgcccgccc gagatggacg acgactcctt cctggtgatg cgccaggcct
     4321 tcgccgggct cctctggagc aagcagcact accgctacga cgtggcccgc tggctgcagg
     4381 gcgaccccgc catgccgccg cccccgcccg agcgcaagca cggccgcaac cacgactggc
     4441 agacgctgaa caataacgac gtcctctcga tccctgacac ctgggagtac ccctggtacg
     4501 cctcctggga tctcgccttc cacaccgtcc ccctggcgat gatcgacccg gagctcgcca
     4561 agcgccagct catcctgctg ctgcgcgagt ggtacatgca cccgaacggc cagctgccgg
     4621 cctacgagtg ggccttctcc gacgtcaacc cgccggttca cgcctgggcc gcctaccgcg
     4681 tctacaagat cgaggagcgc ctgacgggcc gcgccgaccg cctcttcctg gagcgcgtct
     4741 tccagaagct gctgctcaac ttcaactggt gggtcaaccg caaggacgtc gacggccaca
     4801 acgtcttcca gggcggcttc ctggggctgg acaacatcgg cgtcttcgac cgcaccgtgg
     4861 cgctgcccac cggcggccat ctcgaccagg ccgacggcac cgcctggatg ggtctcttct
     4921 gcctcagcat gctggccatc gcgctggagc tggcgcgcac caacatcgcc tacgaggaca
     4981 tgtgcatcaa gttcttcgag cacttcctct acatcgccgg ggcgctcaat agcatcggcg
     5041 aggccgtctc gatctggaac gaggacgacg gcttctacta cgacgtgctg cacctgcccg
     5101 acggcaccaa gaccccgctc aaggtgcgct cgctggtcgg cgtcatcccc ctcttcgcgg
     5161 tcgagacgat cgatcccgag gggatggagg ggctgccgca gttcgccagc gccttccact
     5221 ggttcctggc ccaccgtccc gacctggccg cgctcgtctc gcgctgggat gtccccggcg
     5281 agggccgccg ccgcatgctg gcgctggtcc acggccaccg catgaagcgc gtcctgacgc
     5341 gtgtgctcga ccctcaggag tttctcagcg agtacggcgt gcgctcggtc agccgctacc
     5401 accgcgatca cccctacgtg ctgaccctcg ccggcgcgcg ctacagcgtc gactacgagc
     5461 cgggggaatc gacctccggc gtcttcggcg gcaattccaa ctggcgcggg ccgatctggt
     5521 tcccgctcaa cttcctcctg atcgagtcga tccagaagta ccaccactac tacggcgacg
     5581 acttccgcat cgagtgtccc accggctccg gccgcttcct gacgctctgg caggtggccg
     5641 acgagctctc ctgccgcctg gagtggctct tcacgcgcaa cgccagcggc cgccgcccgg
     5701 tcttcggcga caacgagctc tttcagaacg acccgcgctg gcgcgactac atccccttcc
     5761 acgagtactt ccacggcgag accggcgccg ggctgggcgc cagccaccag accggctgga
     5821 ccgcgctggt ggccaagctc ttggagcaga cgagccgcat gcgcgacctc agcgccgacc
     5881 ggcaggagct gactttggcc gcgagccggc ggtgagtggc gcgaccatcc tcgaccaccc
     5941 gctggtgcgc cgcgggcgcg cgctctgtgg tgagctggag ccggcgctcg gccgcgagtg
     6001 gctggtgacc aacgggctcg gcggctacgc ctcgggcacc gtcgccgggg tcaatacccg
     6061 ccgctaccac ggcctgctgg tggccgcgct caacccgcct gtcgagcgcg tgatgctggt
     6121 cggcggcacg gtggcgtggg ccggctacgg cgggcggcgc tacccgctct ccacgcacga
     6181 gtacgccgac ggcaccgtcg atcccaatgg attcgtcaac atccagtcct ttcacctgga
     6241 gggcgcgctc ccggtctgga cctacgccct cggcgacgcc ctgctggagc ggcgcgtctg
     6301 gatggcccag gggagcaaca cgacctatct cgcctggcgg ctggcgcgcg gctcgctgcc
     6361 gctgcaattg gaggtcacgc cgctgctgac ctatcgtggc ttccacgagc tgcgccgggg
     6421 ccagggctgg cagccggggt gcgcgccggt cgcgcacggc ctgcgcgtcg aggccggcgc
     6481 tggggcgcgg ccctattggc tgctcgcgcc gggcgcggag tacgcgccgg acggcgtctg
     6541 gtggtggaga ttccagcacc gggcggaggc ggcgcgcggg ctggacgcgg tggacgacct
     6601 ctacgcgccc ggcacgttcc ggctcacgct cgcgcccggc cagtccgccg cgctcatctg
     6661 cacgacggag gaggcgcccg tactcgactt cgaagcggcg cttacgggcg agcgggcgcg
     6721 gcagcgggcg ctgctgcaac gggccggggt ggaggcagac gagccggcgc tgcagcagtt
     6781 gacgctggcg gccgatcagt tcatcgtggc ccgcccgccc ggccgctcgc tcatcgccgg
     6841 ctacccctgg ttcggcgact ggggccgcga caccatgatc gcgctccccg gcctgacgct
     6901 ggcgaccggg cggcccgagg aggccgccgc cattctgcgc acgtatgctc ccttcgtgcg
     6961 cgacggcctg ctgcccaacg acttccccaa cgcgcccggc gccgaagtca gctacaccag
     7021 cgccgacgcc gccctgtggt acctgctggc cgcctacgcc tacgaccggc tggccggtga
     7081 tccgtcgctg gtcgacgacc tgctaccgac cttgcgcggc atcgtcgact gctacgccca
     7141 gggcacgcgc tacggcatcg gcgtcgaccc ggccgacggc ctgctgcggg ccggcgcgcc
     7201 ggggctgcag ctcacctgga tggacgtcaa gatcggcgac tgggtggtca cgccgcgcgc
     7261 cggcaagccg gtcgagctgc aggccctctg gtacaacgcg ctgcgcatcc tggccggctg
     7321 gctgcgcgcg cggcacgacc cagcggccgc cggctacgag gccgccgccg accgtgcgca
     7381 cacgtccttc gccgcgcgct tcgtgcggcc gggcctcgac cacctggccg atgtcgtgga
     7441 cgggccggag ggcgacgatc tcgcctgccg cccgaatcag ctcttcgccc tctcgctccc
     7501 cttcgcgctc ctggagggcg atcaagcagc gcgcgtgctc gagtcggccg ggcggcaact
     7561 gctcatcagc tacggcctgc gctctctcgc gcccgctgac ccgcactatg ccgggcgcta
     7621 ccagggcgac ccgcgccagc gcgacgcggt ctaccaccag ggcacggcct gggcctggct
     7681 gagcggcgcc tacatcgacg cctgcgtgcg cgtctacggc agcccggcgc cggccgccgc
     7741 cctgctcgac cccttcaccg accagctctg cgacgccggc ctcggcagca tctccgagat
     7801 cttcgacggc gacccaccgc accacgctcg cggctgcatc gcccaggcct ggagcgtggc
     7861 cgaggtcctc cgctgccgcc gcggcctctt ctggccgaca ctgtagcgat ttgcatcgcc
     7921 gatggtctgc gtggcgccgt tctcccctgt aggggcagac ccctgtgtct gccctgctgc
     7981 cccgacgaca aggccacagg
//
LOCUS       DASUBI010000445         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1220565, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000445 DASUBI010000000
VERSION     DASUBI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220565"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2407)
                     /locus_tag="VFU72_08520"
     CDS             complement(<1..>2407)
                     /locus_tag="VFU72_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU5423568.1"
                     /translation="RVDVPATEPEQAGRRHLLWDCDGETTAWLDGQPWAGLDAAHPTC
                     PLPDRPCTLWLDCGLWESVGGEPLRPIGPYGPRFDQCRLQLRDELAWSVHHDLEVLEQ
                     LRALLYRRDGLATGGAAANAPPLASCSPLLRLLLGGLETVCDAWVAGGLPALVEALRQ
                     LFARLPAEAWQPVAALCGHAHIDLVWLWPEAVTDRKVLHSFANVLRLMERYPELTFVG
                     SQPALYRKVEQAAPAQMREIAARIAEGRWQIAGPFEVEPDSNLPSGEGLARALVYGQR
                     KIAALTGALSDVCWLPDSFGYSASLPQILALGGVRSFFTTKLTWSQITRFPYTSFVWR
                     GPDGSEVLAHLCPTTYNGSVQLEGIAEAARADQQLAVYPEVLLPTGWGDGGGGPTETM
                     CERARRVADLAGMPRARWATADDFFQRMAAVRERLPVYQGELYLEYHRGTLTTQSEFK
                     RLYRGAETALQAHEAARVAIGGEPLGEAAWLRLLFCQFHDALPGSSIRRVYAELNPEL
                     EAIARRELSAAAAELGGGGSGWTAFNPLPLPRAVLVEPPEGNPATPRLLRLGPLERVA
                     LDGQPDDAAPMREIAPTVLDNGIVRATFDRTGRLEGLAVDGQPLALAAPGGFVLYHDQ
                     PTHFDAWDIDHYTARTGVAVADDLELQIGERGPLRGVLRGSAGLGEQSRLRLLYTLEA
                     ESRWLRLEVEVDWHESHRLLRYHVPTAYRGRFARFGSPFGSTLRSQWPGTPADEAMWE
                     VAGSRWAAVTRDDGEGLALLTEAKFGFSCREGNLAVSLLRSPKSPDPEADMGQHRIRL
                     ALGR"
BASE COUNT          330 a          876 c          902 g          299 t
ORIGIN      
        1 ccggcccagc gccaggcgaa tccggtgctg gcccatgtcg gcctcggggt ccggcgactt
       61 ggggctgcgc agcagcgaga ccgcgaggtt gccctcgcgg caggagaagc cgaacttggc
      121 ctcggtcagc agcgccagcc cctcgccgtc gtcgcgggtg acggccgccc agcggctccc
      181 agcgacctcc cacatggcct cgtcggcggg cgtgcccggc cactgcgagc gcaaggtcga
      241 gccgaacggg ctgccgaagc gcgcgaagcg cccccggtag gcggtcggca cgtgatagcg
      301 cagcagccgg tgggactcgt gccagtcgac ctccacctcc aaccgcagcc agcggctctc
      361 ggcctccagg gtgtacaaca ggcgcagccg gctctgctcg cctagccccg cgctgccgcg
      421 cagcacgccg cgcagcgggc cgcgctcgcc gatctggagc tccaggtcgt cggccaccgc
      481 cacgcccgtg cgggcggtgt agtggtcgat gtcccaggcg tcgaagtggg tcggctggtc
      541 gtggtagagg acgaagccgc ccggcgcggc cagcgcgagc ggctgcccat cgacggcgag
      601 gccctccagc cggcccgtgc ggtcgaaggt ggcgcgcacg atcccgttgt ccagcaccgt
      661 gggcgcgatc tcgcgcattg gggcggcgtc gtccggctgg ccgtcgagcg ccacgcgctc
      721 cagcggcccc aggcgcagca ggcgcggcgt ggcgggattc ccctcgggag gctccaccag
      781 caccgcgcgc ggcagcggga gggggttgaa ggcggtccag ccgctgccgc cgccgcccag
      841 ctcggccgcc gccgccgaca gctcgcgccg ggcgatcgcc tccagctccg gattgagctc
      901 ggcgtagacg cgccggatcg agctgccggg gagcgcgtcg tggaactggc agaagaggag
      961 gcgcagccag gccgcttccc cgagcggctc cccgccgatg gcgacccggg cggcctcgtg
     1021 ggcttgcagg gccgtctccg cgccccggta gaggcgcttg aattccgact gcgtggtcag
     1081 cgtgccccgg tgatactcca gatagagctc accctggtag accggcagac gctcgcgcac
     1141 cgccgccatg cgctgaaaga agtcgtccgc ggtggcccag cgggcgcgcg gcataccggc
     1201 caggtcggcg acgcgccgtg cccgctcgca catcgtctcg gtcggcccgc cgcccccatc
     1261 gccccagccg gtcggaagca gcacttcggg atacacggcg agctgctggt cggcgcgcgc
     1321 cgcttcggcg atgccctcca gttggacgct gccgttgtag gtcgtggggc agaggtgcgc
     1381 cagcacctca ctgccgtcgg gcccgcgcca gacgaagctg gtgtagggga agcgggtgat
     1441 ctgcgaccag gtgagcttgg tggtgaagaa ggagcgcacc ccgcccaagg ccaggatctg
     1501 gggcaggctg gccgagtagc cgaagctgtc gggcagccag cagacgtcgc tcagcgcgcc
     1561 ggtcaacgcc gcgatcttgc gctggccgta gacgagcgcg cgcgccagcc cctcgccgct
     1621 cggcaggttg ctgtccggct cgacctcgaa gggcccggcg atctgccagc gtccctccgc
     1681 gatgcgcgcc gcgatctcgc gcatctgcgc cggggcggcc tgctcgacct tgcggtagag
     1741 cgcgggctgg ctgcccacga aggtcagctc cgggtagcgt tccatcagcc gtaggacgtt
     1801 ggcgaaggag tggagcacct tgcggtcggt cacggcctcc ggccagagcc agacaaggtc
     1861 gatgtgggcg tgcccgcaga gggcggcgac cggctgccag gcttcggcgg ggagccgcgc
     1921 gaagagctgc cgcagcgcct cgaccagggc cggcaggccg ccggctaccc aggcatcgca
     1981 gaccgtctcc agcccgccca gcagcagccg caggagcggc gagcaggagg ccagcggcgg
     2041 agcgttagcg gcggcgccgc cggtcgccag cccgtcgcgc ctgtaaagga gggcgcgcag
     2101 ttgctcgaga acctccaggt cgtggtgcac ggaccaggcg agctcgtcgc gcagctgcag
     2161 ccggcattga tcgaagcgcg gcccgtaggg cccgatcggc cggagaggtt cgccgccgac
     2221 cgactcccac aggccgcagt cgagccagag cgtgcagggc cggtccggca gcgggcaggt
     2281 agggtgcgcg gcgtccagcc ccgcccaggg ctgcccatcg agccaggcgg tcgtctcgcc
     2341 gtcgcaatcc cagaggaggt gccggcgccc ggcctgctcc ggctccgtcg ctggtacgtc
     2401 gacccgg
//
LOCUS       DASUBI010000446         9146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_967709, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000446 DASUBI010000000
VERSION     DASUBI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967709"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1280
                     /locus_tag="VFU72_08525"
     CDS             159..1280
                     /locus_tag="VFU72_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423569.1"
                     /translation="MPDVGRPLVLIDGRLIAYRRGGIPRYAAALSRWLPQVAPELDCR
                     LIVNRPTQPSGLPHTRVRTPPHFRFERALLGVELAPRRPALIHSTDFIAPALRASARV
                     ATVHDLAFLEYPEILDAPSRRYYFQLGASVRAADRLITVSAYTAAQLARHFDLSSHQV
                     CVIPNGVDLDRLAPPDDSAAVLRGELGDDIARYIGAKRPLILAVGTVEPRKGYDLLLD
                     SLAQNPLRDMSCVIVGQAGWRAEDLDRRLVLAEASGRLRWLRNASDRVLAALYDSATL
                     LALPSLDEGFGLPALEAMACGLPVVAAASGALPEVIGAAGVLVEERAPEAWAAALAAL
                     AHDATRRLELARRGRERASSFTWRHTAERTAAVYREVLA"
     gene            1277..1894
                     /gene="pth"
                     /locus_tag="VFU72_08530"
     CDS             1277..1894
                     /gene="pth"
                     /locus_tag="VFU72_08530"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5423570.1"
                     /translation="MSDRLTWMIVGLGNPGRQYARTRHNVGFMALDLLCRELPRGTER
                     KRFDARIVETSDSLGRVVLLEPQTFMNRSGLAVAPAARWYKVPSERILVIHDELDLPF
                     GQLRLRPGGSSAGHNGITSIIQQLGTERFPRLRIGVGRPEHGATIGYVLSRFQPEEER
                     LLPDVLARAADAVRVWQRDGIDVAMNQFNRRGAIDATGETGDNRT"
     gene            1952..2803
                     /locus_tag="VFU72_08535"
     CDS             1952..2803
                     /locus_tag="VFU72_08535"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU5423571.1"
                     /translation="MEINRVAVIGAGTMGSGIVQVVAQAQIAVTMIDRESAILDHAMA
                     AIERNLGRSVERGRITQQDADIARGLIETDVSLESARDVDIAIEAANEVLETKLNIFR
                     SLDRICGPESILASNTSSISITRLGAATARPERVIGMHFFNPVPVMQLVEVVRGLATS
                     DETTNTVTGFGERLGKTPVTIEDFPGFISNRVLMPMINEAIYCLLEGVADAESIDTVM
                     KLGMNHPMGPLALADLIGLDVCLDIMEVLHHDLGESKYRPCPLLRKMVAAGRLGRKSG
                     QGFFNYG"
     gene            2812..3963
                     /locus_tag="VFU72_08540"
     CDS             2812..3963
                     /locus_tag="VFU72_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5423572.1"
                     /translation="MDFALDEEHRLLRAAVEEFAARDVAPRVRERDQEARFPTEEVAD
                     AARNGLLGLLVPEQDGGTAVGTLGAAIVYEEVARVSPSLSVILSVHNSLVCGALVEYG
                     GDELKARLLPQLASGALLGAYALTESQAGSDAAAIRTSALKAPDGYRISGRKQFITSG
                     AHAGLYIVFAVTDPAASTSRRISAFAVEASRPGVRVVRVEPKLGLRASEIVELEFDDV
                     LVPPDHRLGQEGYGFGIAMSLLNGGRIGIGAQSVGIARGAFERALRYAGERQQFGQPI
                     GHFEAIQIKLADMAMRISAARLLVYRAAWLRDSGQEHALAASEAKLFASEAAVAVADA
                     AVQIHGGYGYLEEFEVERYLRDARATTLYEGTSEIQRLVIARRLLAGQN"
     gene            3979..4539
                     /locus_tag="VFU72_08545"
     CDS             3979..4539
                     /locus_tag="VFU72_08545"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU5423573.1"
                     /translation="MSQETTKNGQEARSYLMLLVGDERATSDAALGIALRASGYSLRV
                     HIIEFQKTGRERGEVVAASLLTGLSLTQYGTLTSERTPTESNDLGTTAERAEAAMREA
                     GVHVRQRVTNILILDGVLSLVKAGLVTDTAVLELVEMAAPWLDVVATGLEASERLKKA
                     ANSLTLMETIKETTHAVRDLRRGIHY"
     gene            4561..6207
                     /locus_tag="VFU72_08550"
     CDS             4561..6207
                     /locus_tag="VFU72_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5423574.1"
                     /translation="MRDDRRRENPDYDLPPDPGRRDFTTVSGEPVEPVYGPAELERLG
                     FEAARDLGQPGEFPFTRGIHQSMYRGQLWTMRMFAGFGSAEETNARFRYLLEHGETGL
                     SVAFDLPTLYGRDTDDPLAEGEFGKCGVAVSSLRDFEILFDRIPQEAITTSMTINGPA
                     AIIWAMYIALADRRGIARERLGGTLQNDILKEYTAQNEYIFPPEPSMKLVVDTIEFAT
                     HEMPRWNSISVSGYHIREAGATATEELAFTLADGMAYVEAALARGLDIDDFAPRISFF
                     FNCHNDFFEEIAKFRAARRIWARVMRDTYGAKNPRSWWMRFHTQTAGCSLTAQEPENN
                     VIRTTIQALAAVLGGTQSLHTNSLDEALALPSESAVRTALRTQQIIAFESGVTNSVDP
                     LAGSYLVETMTSEMEQRVEAIFAEIEELGGVLAAVESGYFQKRIADSAYRYEQALAER
                     RKIVMGVNALRSHETPDIEFLQMDPQGEARQRARLAQLRTERDEARWAESLARLEDDA
                     RAGRNLMPALIDAVKADATLGEITNRLKVVYGAYRPLQVV"
     gene            6244..6666
                     /locus_tag="VFU72_08555"
     CDS             6244..6666
                     /locus_tag="VFU72_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5423575.1"
                     /translation="MVQTHPRPIRVLIAKPGLDGHDRGAKVMVRALRDAGMEVIYTGL
                     FQTPEMIAKAAADEDVDVVGLSILSGAHNTLIPRIVAELRGADMADVLVVVGGTIPEQ
                     DVAFLHEQGVEGVFGPGTPLSEAIDFIRAHAPVREEQL"
     gene            6663..7619
                     /gene="meaB"
                     /locus_tag="VFU72_08560"
     CDS             6663..7619
                     /gene="meaB"
                     /locus_tag="VFU72_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5423576.1"
                     /translation="MSGLLERFRAGDRRALSRLMSYVENRSPLGQEALKALFPLTGRA
                     HLVGITGPPGAGKSTLVNALIREERERGRTVGVLAIDPSSTLTGGAALGDRIRMMENY
                     ADEGVFIRSMATRGQIGGLSLAVSGAAHLLDAFGFDLILIETVGVGQDEVDIADAADT
                     TLVVQVPGLGDSIQTIKAGVLEIADILVVNKADRPEARQLVRDLRNMLLLSEHGAAMP
                     PIVSTVAHEGKGVAKLADEIDAQRARLEASGGAQVRRGRRLRREVELLAGEQFDRWLR
                     ERLRALRAERAGEELVARELDPGSLAARLVEEFRALDQPAQR"
     gene            complement(7634..8830)
                     /locus_tag="VFU72_08565"
     CDS             complement(7634..8830)
                     /locus_tag="VFU72_08565"
                     /inference="COORDINATES: protein motif:HMM:NF004636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5423577.1"
                     /translation="MAEELLLRHVRPQHGAAIDLLVVDGRVAALGPGLKPASPATTVI
                     DGGNRLLFPGFVDAHAHMDKTLLGLGWYRNEVGPSLIDKIENERSLRRERDIDAHQQS
                     TRQARRSISAGTTAIRTFVDIDTETRLSGFEGVMRTRADFRDSLDIQVVAFPQSGMLI
                     RPGTIELMETALREGATVVGGLDPSAIDRDPVKHLDVVFGLAEKYDVDVDIHLHEPAE
                     LGAFSLELIAERTRTLGWQGRVVVSHAFCLGAVDDNYLNRLIELVRENRIAIMSHGPS
                     GGRPAPPVKRLVEAGVTVCAGNDGIRDAWGPLNMPDMLLRAFLIAYRNNFRRDDEIEL
                     TLDIITRGGEAVLGMPAGSLATGSPGDFVIVDGETHVEAVIERPPRWLVIKRGRIVAR
                     EGECLV"
     gene            complement(8864..>9146)
                     /locus_tag="VFU72_08570"
     CDS             complement(8864..>9146)
                     /locus_tag="VFU72_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEU5423578.1"
                     /translation="IVAAGMDGALPSVVAGLVPLPVVGLPTSVGYGLGGNGQGALLSM
                     LQSCAPGLTVVNIDNGVGAGAAAGMVANAVAAARSAGLNGSSEPTNENG"
BASE COUNT         1475 a         3130 c         3066 g         1475 t
ORIGIN      
        1 tccgggcgtg atctacgcac tctggattct ggcccaccgc ccggcgctgc gctcgccggc
       61 agggctggag gagtatccgc tctagcaggt ttggccggtc gattcgcggc tggggtacga
      121 tctgctcgga ggcgcgggtt cattggcgga ggcgcgacgt gccggacgtg ggaaggccac
      181 tggtgctgat cgatgggcgc ctgatcgcct atcggcgcgg cggcatccca cgctacgcgg
      241 cggcgctgtc acgctggttg ccccaggtcg cgcccgaact cgactgccgg ttgatcgtca
      301 accgccccac ccaaccgtcg ggcctgcccc atacgcgcgt ccgcacgccg ccgcacttcc
      361 gcttcgagcg tgccctgctt ggcgtcgagt tggccccgcg ccgtcccgcg ctgatacaca
      421 gcaccgactt catcgccccg gcgctacgag ccagcgctcg ggtcgcgacc gtgcacgacc
      481 tcgccttcct ggagtacccc gagattctcg acgcgccgag ccgccgctat tactttcagc
      541 tcggcgcgtc ggtacgggcg gccgaccgct tgatcacagt ctcggcctac accgcggcgc
      601 agttggcccg acacttcgat ctatcgtcgc atcaggtctg cgtcatcccc aacggagtcg
      661 atctcgatcg cctcgcccct cccgatgact cggcagcggt gctgcgaggc gagctcggcg
      721 atgatattgc ccgctacatt ggtgccaaac gaccgttgat actggccgta gggactgtag
      781 aaccacgcaa agggtatgac ctcctcttgg acagtttggc tcaaaaccca ctcagggata
      841 tgtcgtgtgt gatcgtcggg caggcgggct ggcgcgccga ggatctggac cgacgcttgg
      901 ttctggccga agcgtccggc cgtctccgct ggctgcgcaa cgccagcgac cgcgtcctcg
      961 cggcactcta cgactccgcg acgctcctgg cgctcccctc tctcgacgag gggttcgggt
     1021 tgccggcgct cgaagcgatg gcttgcgggc tgccggtggt cgccgctgcc tccggcgcgc
     1081 tgcccgaggt catcggtgcg gcaggggtac tggtcgagga gcgcgcgccg gaagcctggg
     1141 ccgccgcgct cgccgcgctg gcgcacgacg ccacacgccg tctggagctg gcccggcgcg
     1201 gccgcgagcg tgccagctcc ttcacctggc ggcacaccgc cgagcgcacg gccgcggtct
     1261 accgtgaggt gctggcttga gcgaccggct gacttggatg atcgtggggc tgggcaaccc
     1321 tgggcgccaa tatgcgcgca cgcgccacaa cgttggcttc atggcgctcg accttctgtg
     1381 tcgcgagctg ccgcggggca ccgagcgcaa gcgcttcgat gcccgtatcg tcgaaactag
     1441 cgactcgctc ggacgtgtcg tgctgctgga gccgcagacc ttcatgaacc gcagcgggct
     1501 ggcggtcgcg ccagcggccc gctggtataa ggttccctcc gagcgcattc tggtgatcca
     1561 cgacgaactc gatctcccct tcggccagct ccggctgcgc ccgggcggca gcagcgccgg
     1621 tcacaacggc atcacgtcga tcatccagca gctcggcacc gagcgtttcc cccgcctgcg
     1681 catcggtgtc ggccgtcctg agcatggcgc caccatcggt tatgtgttga gccgcttcca
     1741 gccggaggag gagcgcctct tgcccgacgt actggcccgc gccgccgatg ccgttcgcgt
     1801 ctggcagcgc gacggtatcg acgttgcgat gaatcagttc aaccgccgag gggcaatcga
     1861 cgcgaccggc gaaaccggag acaatcggac atagacacgc cgcgcgcgag atggcacgct
     1921 gtgctggtgg aaggaaggtg cggcacgagg catggagatc aatcgggtcg ccgtcatcgg
     1981 cgcgggaact atgggcagcg gcatcgtgca ggtggtggcg caggcgcaga tcgcggttac
     2041 gatgatcgac cgggagagcg ccatcctcga tcacgccatg gccgccatcg agcgcaatct
     2101 gggccgctcc gtcgagcgcg gccgcatcac ccagcaggat gccgacatcg cgcgtgggct
     2161 catcgagacc gatgtgagcc ttgagagcgc gcgcgacgtc gacatcgcca tcgaagcggc
     2221 gaacgaggtc ctggagacca agctcaacat ctttcgctcg ctggatcgta tctgcgggcc
     2281 ggagtcgatc ttggcctcca acacgtcttc gatctcgatt acgcgcctcg gcgcggcgac
     2341 cgctcgcccg gagcgggtca tcggcatgca tttcttcaac ccggtgccgg tgatgcagtt
     2401 ggtcgaggtc gtgcgcggct tggcgacctc ggacgagacc accaacaccg tgaccggctt
     2461 cggcgaacgc ctcggcaaga cgccggtgac gatcgaggat tttcctgggt tcatctccaa
     2521 ccgcgtcctg atgccaatga tcaacgaggc gatctattgc ctgctcgaag gcgtcgccga
     2581 cgccgagagc atcgatacgg tgatgaagct cggcatgaac catcccatgg ggccgctggc
     2641 gctggccgat ctcatcggac tcgacgtctg cctcgacatc atggaagtgc tgcaccacga
     2701 cctcggtgag tcgaaatatc gcccctgccc actgctgcgc aagatggtcg cggccgggcg
     2761 gctcggccgc aagtccgggc aaggtttttt caactacggc taggcaagac gatggatttc
     2821 gcgctcgatg aggagcaccg gctgctgcgt gcggcggtgg aagagttcgc cgcccgggat
     2881 gtcgcaccgc gcgtgcgtga gcgcgaccag gaggcacgct tccccaccga ggaagtcgcg
     2941 gacgcggcgc gtaacggcct gctcgggctg ctcgtgccgg agcaagacgg cggcacggcg
     3001 gtcggcacgc tcggagcggc gatcgtctac gaggaggtcg cgcgcgtctc gccttccctg
     3061 tcggtcatcc tctcggtcca taactcgctg gtctgtggcg cgctggtcga atacggcggt
     3121 gacgagctga aagcgcggct cttgccgcag ctcgccagcg gcgctctgct cggcgcctac
     3181 gccttgaccg agtcgcaggc cggttccgac gccgccgcga tccgcaccag cgcgctgaaa
     3241 gcgccggacg gctaccgcat cagcggacgc aagcagttca tcacctccgg ggcccacgcc
     3301 gggctgtaca tcgtcttcgc cgtgactgat cccgcggcca gcacctcgcg acgcatctcg
     3361 gccttcgcgg tcgaggcgag ccggcccggg gttcgcgtgg tccgcgttga gccaaaactc
     3421 ggcttgcggg cctccgagat cgtggagctc gagttcgacg acgtgctcgt gccgcccgat
     3481 caccggctgg gccaggaggg gtacggcttc ggcatcgcca tgagcctgct caacggcggc
     3541 cgcatcggca tcggcgcgca gtcggtaggc atcgcgcgcg gggccttcga gcgcgcgctg
     3601 cgctacgccg gcgagcgcca gcagttcggc cagccgatcg gtcacttcga ggcgatccag
     3661 atcaaactgg ccgacatggc catgcgtatc tctgccgcgc gcctgctggt ctatcgcgct
     3721 gcctggctgc gcgacagcgg tcaggagcac gctctagcag cctccgaggc caagctgttc
     3781 gcttccgaag cagccgtcgc ggtggccgat gctgcggttc agatccatgg ggggtatggt
     3841 tacctggagg agttcgaagt cgagcgttac ctgcgggacg cgcgcgcgac caccctctac
     3901 gagggcacgt cggagatcca gcgtctggta attgcccggc ggcttctggc gggtcagaat
     3961 taggacggtg gaggtgcgat gagtcaggag accacgaaga acggtcagga agcgcgcagc
     4021 tacctcatgc tgctggtggg tgacgagcgc gccacttcag acgccgcgct ggggatcgcc
     4081 ttgcgcgcgt ccggctacag cttgcgcgtg cacatcatcg agttccaaaa gaccggccgg
     4141 gagcggggcg aggtggtggc cgcctcactt ctcaccgggc tctcgctcac ccagtacggc
     4201 acgctgacct ccgagcgaac cccgaccgag tccaacgatc tgggaacgac cgccgagcgc
     4261 gccgaggcgg ccatgcgcga ggccggagtc cacgtgcgcc agcgcgtgac caacatcctg
     4321 atcctggacg gtgtgctctc cctggtcaag gccgggctgg tgaccgacac cgcggtcctg
     4381 gagctggtcg agatggccgc tccctggctc gacgtcgtcg cgacgggtct ggaggcgagc
     4441 gagcggctca agaaggcggc caacagcctg acgctcatgg agaccatcaa ggagacaacc
     4501 cacgcggtgc gcgatctccg tcgtggcatc cactactagc agctaggaag gcgacccgcc
     4561 atgcgcgacg atcgacgccg ggagaatccc gattacgacc tgccacccga ccctgggcgc
     4621 cgcgacttca ccaccgtctc cggcgagccg gtcgagccgg tttacggccc cgccgaactg
     4681 gagcgcctgg gctttgaagc ggcgcgcgat ctgggccagc cgggtgagtt tccctttacc
     4741 cgcggcatcc accagtcgat gtaccggggc cagctctgga cgatgcgcat gttcgccggt
     4801 ttcggctcgg ctgaggagac caacgcgcgc ttccgctacc tgctggagca cggcgagacc
     4861 ggactttcgg tggccttcga tctcccgacc ctctacggtc gcgacaccga cgatccgttg
     4921 gccgaggggg agttcggcaa atgcggcgtc gcggtctcgt ccctacgcga cttcgagatc
     4981 ctcttcgacc gcatcccgca ggaagccatc acgacttcga tgacgatcaa cggtccggcg
     5041 gcgatcatct gggctatgta tatcgcgctg gccgaccgcc gcggcatcgc gcgcgagcgg
     5101 ctcggcggca ccttacagaa cgatatcctc aaggagtaca ccgcccaaaa cgaatatatc
     5161 ttcccgcccg agccctcgat gaagctggtg gtcgacacca tcgagtttgc cacccacgag
     5221 atgccgcgct ggaacagcat cagcgtcagc ggctaccaca tccgtgaggc gggcgcgacc
     5281 gccaccgagg aactggcctt cacgctggcc gacggcatgg cctacgtcga agcggccctg
     5341 gcgcgcgggc ttgatatcga tgacttcgcg ccgcgcatct cgttcttctt caactgtcat
     5401 aacgacttct tcgaggagat cgccaagttc cgcgccgcgc gccgtatctg ggcgcgcgtc
     5461 atgcgcgaca cctacggcgc caagaaccca cgctcctggt ggatgcgctt tcacacccag
     5521 accgccggct gttcgctgac ggcgcaggag ccggagaaca acgtcatccg caccactatc
     5581 caggcgctgg cggcggtgct cggcggtacc cagtcgctgc acaccaactc gctcgacgag
     5641 gcgctcgcgc tgccgtcaga gagcgcggtg cgcacggcgc tgcgtaccca gcagatcatc
     5701 gccttcgaga gcggcgtcac caacagtgtc gaccccctgg ccggcagcta cctggtcgag
     5761 acgatgacca gcgagatgga gcagcgggtc gaggcgatct tcgcggagat cgaagaactg
     5821 ggcggggtgc tggcagcggt cgagtcgggc tatttccaaa agcgcatcgc ggactccgcc
     5881 taccgctacg agcaggcgct ggccgagcgg cgcaagatcg tgatgggcgt gaacgcgttg
     5941 cgctcgcacg agacgccgga catcgagttc ttgcagatgg atccccaggg cgaggcgcgc
     6001 cagcgagcac ggctggccca actgcgcacc gagcgcgacg aggcgcgctg ggccgagtcg
     6061 ctggcccggc tggaggacga cgcgcgcgcg gggcgcaacc tgatgccggc cctgattgac
     6121 gcggtcaagg ccgatgccac gctgggcgag atcaccaacc gcctgaaggt cgtctacggc
     6181 gcctatcggc cgttgcaggt ggtctaaacc ggtggtgagt ttgccgaggg agaggagcag
     6241 gcggtggttc agacgcaccc gcggccgatt cgcgtgctga tcgccaagcc ggggctcgat
     6301 gggcacgatc gcggcgccaa agtaatggtg cgcgcgctgc gtgacgccgg catggaagtc
     6361 atctacaccg ggctcttcca gacgcccgag atgatcgcca aggcggccgc cgacgaggat
     6421 gttgatgtcg ttggcctgag catcctctcc ggcgcgcaca acacactcat cccccgtatc
     6481 gtcgcagagc tgcgcggggc ggacatggcc gacgtgctgg tggtcgtcgg cggcaccatc
     6541 cccgagcaag atgtcgcttt tctgcacgag cagggtgtcg agggcgtctt cggtcccgga
     6601 acgccgctca gcgaggcgat cgacttcatc cgggcacacg cgcccgtccg cgaggagcag
     6661 ctttgagcgg cttgctcgag cgctttcgtg ccggcgaccg ccgcgcgctg tcgcgcctga
     6721 tgagctacgt cgagaaccgc tcgccgctag ggcaagaggc gctcaaggcg ctcttcccgc
     6781 tgaccggccg agcccacctg gtcggcatca ccggcccgcc cggcgccggc aagagcacgc
     6841 tggtcaacgc gctgatccgc gaggaacgcg agcgtggccg gacggtcggc gtgctggcga
     6901 tcgacccctc cagcacgctc actggcggcg ccgcgctcgg cgaccgcatt cgtatgatgg
     6961 agaactacgc cgacgagggc gtcttcatcc gcagtatggc tacgcgcggc cagatcggcg
     7021 gcctctcgct ggcggtcagc ggcgcggccc atctgctcga cgccttcggc ttcgacctga
     7081 tcctgatcga gacggtcggc gtcggccagg acgaggtcga catcgccgac gcggccgaca
     7141 cgacactggt ggtgcaggtg ccgggcctgg gtgacagcat tcaaacgatc aaagccggtg
     7201 tgctggagat cgcggacatc ctcgtagtca acaaggccga ccgtccggag gcacgtcaat
     7261 tggtgcgcga cctgcgcaac atgctcctgc tcagcgagca cggcgcggcc atgccaccga
     7321 tcgtgtccac ggtcgcccac gagggcaagg gcgtagccaa actggccgac gagatcgacg
     7381 cgcagcgtgc ccggctcgaa gccagcgggg gcgcccaggt gcggcgcggc cggcgcctgc
     7441 ggcgtgaggt ggagctactg gccggcgagc aattcgatcg ctggctgcgt gagcggttac
     7501 gcgcgttgcg cgccgagcgc gccggcgaag aactggtcgc ccgtgagctc gatccgggaa
     7561 gcctggcagc gcgcttggtc gaggagtttc gcgcgctcga tcaaccggcc cagcggtagg
     7621 tgccggggcg agtttagacc aggcactccc cctcgcgcgc cacgatccgg ccgcgcttga
     7681 tcaccagcca gcggggcggc cgctcaatca ccgcctcgac atgcgtctca ccgtcgacaa
     7741 tgacgaaatc gcctggtgaa ccggtcgcca ggctgccagc aggcattccc agcaccgcct
     7801 cacccccgcg cgtgatgatg tcgagcgtca gctcgatctc atcgtcgcgg cggaagttat
     7861 tgcgataggc gatcaagaac gcgcgcagca gcatgtccgg catgttgagc ggtccccagg
     7921 cgtcgcggat gccgtcgttg ccggcgcaga cggtcacgcc ggcctcgacc agccgcttga
     7981 ccggcggggc cggccgtccg ccgctcgggc cgtggctcat gatcgcgatg cggttctccc
     8041 gtaccagctc gatcaaccgg ttgaggtaat tgtcgtcgac ggcgccgagg cagaaggcat
     8101 ggctgacgac gacccgaccc tgccagccga gcgtgcgcgt gcgctcggcg atcagctcga
     8161 gcgagaaggc gcccaactca gccggctcgt gcaggtggat gtcgacgtcg acgtcgtact
     8221 tctcggccag gccgaagacg acgtcgagat gcttcaccgg gtcgcggtcg atcgccgagg
     8281 ggtcgaggcc accgacgacc gttgccccct cgcgcagcgc cgtctccatc agctcgatgg
     8341 tgccagggcg gatcagcatg ccgctctgag ggaaggcgac gacctgaatg tcgagcgagt
     8401 ccctgaagtc ggcgcgcgtc cgcatgactc cctcgaagcc ggagagtcgc gtctcggtgt
     8461 cgatgtcgac gaaggtgcga atggccgtcg tgccagccga gatcgaccgg cgcgcctgcc
     8521 gcgtcgactg ttgatgggcg tcgatgtcgc gctcgcggcg caagctacgc tcgttctcga
     8581 tcttgtcgat cagactcggc ccaacctcgt tgcggtacca gcccagcccg agcagggtct
     8641 tgtccatgtg ggcgtgcgcg tccacaaacc ccgggaagag caggcggttg ccgccgtcga
     8701 tcacggtcgt tgcgggggat gccggcttca ggcccggccc aagcgccgcc acacgcccat
     8761 cgaccactag gagatcgatc gccgcgccgt gctgcggccg gacatgccgt agcagcagtt
     8821 cttccgccat cttccctacc cctccgcgac cggcgcccgc gactcagccg ttctcgttgg
     8881 tgggctcgga agagccgttc agccccgcgc tcctcgccgc cgcgaccgcg ttcgcgacca
     8941 tccccgccgc cgcaccggca cccaccccat tatcgatatt gaccacggtc aaacccggcg
     9001 cgcaactctg caacatgctc aagagtgctc cctggccgtt cccgcccagt ccgtaaccaa
     9061 ccgaggtcgg cagcccgacc accggcagcg gcaccaggcc ggcgaccacc gagggcagcg
     9121 cgccatccat cccggcggcc acgatg
//
LOCUS       DASUBI010000447         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1228139, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000447 DASUBI010000000
VERSION     DASUBI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228139"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFU72_08575"
     CDS             <1..660
                     /locus_tag="VFU72_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5423579.1"
                     /translation="ARRRAGLILTARGESALAGAARELGTLTEVLALPGDVADPRHAA
                     RLVDEGLNRFGRIDVLINSASSIGPSPMPELDAYPLDRLAEVFAGNVIAPLHLIQLVL
                     PQMRARGDGVIVNVSSDAAVEAYPGWGGYGASKAALEHLSRVLAAELVGSGVRVYAVD
                     PGDMDTQMHREAEPGVDLSHLPGPEVPARAFVRIITEERAPYARFEAQKLVAAGLGGV
                     R"
     gene            657..1676
                     /locus_tag="VFU72_08580"
     CDS             657..1676
                     /locus_tag="VFU72_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEU5423580.1"
                     /translation="MSVLTERLHFTLPPELEAHEPPEARGLARDQVRLLVSRQSDDRL
                     THAQFRDLPRFLRPGDLLVANDSATLPAALTVHDAVGAEFCLHLSTRLTGDLWAVEPR
                     GAEARARAELSLPGGGAATLLAPYGGSKRLWVARLELPEEPFAYLARWGWPIAYSYLH
                     RRWPIELYQTVYARVPGSAEMPSAGRPFTRAILQRLARQGVGFATLTLHTGVASLEAH
                     ELPYPERFRVPLETAEAVRATRAAGGRVIAVGTTVVRALESAVDAAGRVIASQGWTDL
                     VITPERGVRVVDGLLTGFHEPEATHLAMLAAIASRHHLELAYQAALEHGYLWHEFGDL
                     HLLLP"
     gene            1692..2099
                     /locus_tag="VFU72_08585"
     CDS             1692..2099
                     /locus_tag="VFU72_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423581.1"
                     /translation="MRITGTHHVALATPDLAQLRKFYVDTLGLPVVGGFPTHGILFVR
                     AGETVIELVAEDVPAGGARRGGWDHLALEVADVDTAYAALLARGAAPHSPPEDFPPLP
                     ENPALRIAFLCDPDGNLLELVQPLAARYPPTSR"
     gene            2157..2996
                     /locus_tag="VFU72_08590"
     CDS             2157..2996
                     /locus_tag="VFU72_08590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5423582.1"
                     /translation="MLFGVHVAPSARTLDIREVGRLAEAAGFESLFLPEHTHMPAYGA
                     SVHPSGEPMRERLSGFLDPFTALAAVAAVTERLRLGTGVCLITQHDPITLAKTCSTLD
                     LLSRGRLILGVGAGWNREELANHGTEPRRRWALMRERVLALKQIWTEEPAEFHGDLVD
                     FGPILQGPKPVQRPHPPVLIGGEGPRVLERVLDYGDGWGPNAEPGIPERIGELQRLAH
                     ERGKEPIPVTAFHVPPALEQIRRYAEAGTDRCVFTLPEAGRDEVEAALDRLRRLVTRA
                     QAG"
     gene            complement(3001..4446)
                     /gene="aspA"
                     /locus_tag="VFU72_08595"
     CDS             complement(3001..4446)
                     /gene="aspA"
                     /locus_tag="VFU72_08595"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEU5423583.1"
                     /translation="MVDSALRYEHDLLGDRAVPTEAYYGVHTVRAVENFPITGTPISV
                     YPELIEALASVKQAAALANRDLGLLDAKKADAIVRASEEIRQGALLDQFAVDVIQGGA
                     GTSTNMNANEVIANRALEHLGLERGDYKHLHPIEHVNMSQSTNDVYPTAVKVALQVMI
                     GELAAAMSVLRGAFEAKAAEFADVLKMGRTQLQDAVPMTLGQEFGAFGIMIGEDEERL
                     REAALLIREINLGATAIGTGINTHPDYAELARRHLAAITGIPLITAANLVEATQDAGA
                     FVQLSGVLKRIAVKLSKISSDLRLLSSGPRAGLGEINLPAVQAGSSIMPGKVNPVIPE
                     VVNQIAYEVIGNDVTVSFAAEGGQLQLNAFEPIIAHSLFGSLQHLRAGCLTLAERCVR
                     GISANRDKLRQSVEHSIGIVTALNPYIGYENATRIAQEALSSGRGVAELVLEHGLMTK
                     EQLDKILRPEVLTRPRPALSDDWAARRDEGR"
     gene            4888..5127
                     /locus_tag="VFU72_08600"
     CDS             4888..5127
                     /locus_tag="VFU72_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423584.1"
                     /translation="MKERPRTRFWVEAGFATLSLIFAVLTLIWKDWIEIVFRIDPDNH
                     SGSLEWLIAFAALAIAIIIAGVARAEWRHTASATA"
BASE COUNT          760 a         1849 c         1817 g          810 t
ORIGIN      
        1 gcccggcgcc gggccggctt gatcctgacc gcgcgcggcg agtcggcgct ggccggggcg
       61 gcgcgcgaac tggggacact gacggaggtg ctggcgctgc cgggcgacgt ggccgatccg
      121 cggcacgccg cgcggctggt ggacgagggg cttaatcgct tcgggcgcat cgacgtgctg
      181 atcaacagcg cctcctcgat cggccccagc ccgatgccgg agctggatgc ctacccgctc
      241 gatcgcctgg ccgaggtctt cgccggcaac gtcatcgccc cgctccacct gatccagctc
      301 gtgctgcccc agatgcgggc gcgcggcgac ggcgtgatcg tcaacgtcag ctccgacgcg
      361 gcggtcgaag cctaccccgg ctggggcggc tacggcgcca gcaaggcggc gctggaacac
      421 ctctcgcgcg tgctggcagc cgagctggtg gggagcggcg tgcgcgtcta cgccgtcgac
      481 cccggcgaca tggacaccca gatgcaccgg gaggccgagc cgggcgtcga cctgtcgcac
      541 ctgccggggc cggaagtgcc ggcgcgcgcc ttcgtgcgga tcatcaccga ggagcgggcg
      601 ccctacgccc gtttcgaggc ccagaagctg gtcgcggcgg ggctgggagg tgtgcgatga
      661 gcgtgctcac cgagcggctg cacttcactc tgccgcccga gctggaggcc cacgagccgc
      721 ccgaggcacg cgggctggcg cgcgatcagg tgcggctgct ggtcagccgt caatcagacg
      781 atcgcctgac gcacgcccag ttccgcgacc tgccgcgctt tctgcggccc ggcgacctgc
      841 tggtggccaa cgactcggcc accctccccg ccgcgctgac cgtgcacgac gccgtcggcg
      901 cggagttctg cctgcatctc tcgacccggc tgaccggcga cctctgggcg gtcgagccgc
      961 gcggggccga agcgcgggcc cgtgccgagc tgtcgctgcc gggcgggggc gcggccacgc
     1021 tgctggcgcc ctacggcggc tcgaagcggc tctgggtggc gcggctggag ctgccggagg
     1081 agccgttcgc ctacctcgcc cgctggggct ggccgatcgc ctactcctac ctgcaccgcc
     1141 gctggccgat cgagctctat cagacggtct acgcgcgcgt gccgggctcg gccgagatgc
     1201 cctcggcggg acggcccttc acacgggcca tcctgcagcg gctggcgcgg cagggcgtcg
     1261 gcttcgccac cctgacgctg cacaccggcg tcgccagcct ggaggcgcac gagctgccct
     1321 accccgagcg gttccgcgtg ccgctggaga ccgccgaggc ggtgcgcgcc acgcgcgcgg
     1381 cgggtgggcg cgtgatcgcg gtgggcacca ccgtcgtgcg cgcgctggag agcgcggtcg
     1441 acgccgcggg ccgggtcatc gccagccagg gctggaccga cctggtgatc acgcccgagc
     1501 gcggcgtgcg cgtggtcgac ggcctgctca ccggcttcca cgagccggag gccacccacc
     1561 tggcgatgct ggcggccatc gccagccgcc accacttgga gctggcctac caggccgcgc
     1621 tggagcacgg ctacctctgg cacgagttcg gtgacctgca cctgctcctg ccgtaacgag
     1681 ggggcaacca agtgcgcatc acgggcaccc accacgtcgc gctggccacg ccggacctgg
     1741 cccagctgcg taagttttat gtcgacacac ttggtctgcc ggtggtgggc ggcttcccca
     1801 cgcacggcat cctcttcgtc cgcgcgggcg agacggtcat cgagctggtc gccgaggacg
     1861 tgccggcggg cggcgcgcgg cgcggcggct gggatcacct ggcgctggag gtcgcggacg
     1921 tggacaccgc ctacgccgcg ctgctggcgc gcggggccgc gccgcactcg ccgcccgaag
     1981 atttcccgcc cctaccggag aaccccgcgt tgcgcatcgc cttcctgtgc gaccccgacg
     2041 gcaacctgct ggagctggtc cagccactcg ccgctcgcta tcctccaacg tcacgctgat
     2101 cgctgcccat cggcatttcc gcggtgaaga aggcagtaga gggggaggga cgagccatgc
     2161 tctttggagt acacgtggcg ccgagcgcgc gcacgctcga cattcgcgag gtcgggcggc
     2221 tggccgaggc ggccggcttc gagtcgctct tcctgccgga gcacacccac atgccggcct
     2281 acggagcgtc ggtccatccc agcggcgagc cgatgcgcga gcgactgtcc ggcttcctgg
     2341 atccgttcac cgcgctggcg gccgtggcgg cggtcaccga gcggctgcgg ctgggcaccg
     2401 gcgtctgcct gatcacgcag catgacccga tcacgctcgc caagacgtgc agcacgctcg
     2461 atctcctgtc gcgcggccgc ctgatcctcg gcgtcggcgc cggctggaac cgtgaggagc
     2521 tggccaacca cggcaccgag ccgcgccgcc gctgggcgct gatgcgcgag cgggtgctgg
     2581 cgctcaagca gatctggacc gaggagccgg ccgagtttca cggagacctc gtcgatttcg
     2641 ggccgattct gcaaggcccg aaaccggtgc agcggccgca cccgccggtg ctgatcgggg
     2701 gcgagggccc gcgcgtcctc gagcgcgtgc tcgactacgg cgacggctgg gggccgaacg
     2761 ccgagccggg catccccgag cgcatcggcg agctccagcg gctggcgcac gagcggggga
     2821 aggaacccat ccccgtcacc gccttccacg tgccgcccgc tctggagcag atccggcgct
     2881 acgccgaggc gggcaccgac cgctgcgtct tcaccctgcc ggaggcgggc cgcgacgagg
     2941 tggaggcggc gctcgaccgc ctccgccgcc tcgtcacgcg cgcgcaggcc ggctgagtgg
     3001 ctaccgcccc tcgtcgcgcc gggcggccca atcgtcggag agggcggggc gcgggcgggt
     3061 cagcacctcc ggccgcagga tcttgtcgag ctgctccttg gtcatcaggc cgtgctccag
     3121 caccagctcg gcgacccccc ggccgctgct cagcgcctcc tgcgcgatgc gggtggcatt
     3181 ctcgtagccg atgtaggggt tgagcgcggt cacgatgccg atcgagtgct cgacgctctg
     3241 gcgcagcttg tcgcgattgg cgctgatgcc gcgcacgcag cgctcggcca gcgtcaggca
     3301 gccggcgcgc aggtgctgca ggctgccgaa gagggagtgg gcgatgatcg gctcgaaggc
     3361 gttgagctgg agctgcccgc cctcggcggc gaagctgacc gtcacgtcgt tgccgatcac
     3421 ctcgtaggcg atctggttga ccacctccgg gatgaccggg ttgaccttgc ccggcatgat
     3481 gctggagccg gcctgcaccg ccggcaggtt gatctcaccc agcccggcgc gcggcccgga
     3541 ggagagcagg cgcaggtcgc tgctgatctt ggagagcttg accgcgatgc gcttgagcac
     3601 gcccgagagc tgcacgaagg cgccggcgtc ctgggtcgcc tccaccagat tggcggcggt
     3661 gatcagtggg atgccggtga tcgcggccaa gtggcgccgc gccagctcgg cgtagtcggg
     3721 gtgggtgttg atgccggtgc cgatggcggt ggcccccaga ttgatctcgc ggatcagcag
     3781 cgccgcctcg cgcaaccgct cctcgtcctc gccgatcatg atgccgaagg cgccgaactc
     3841 ctgcccgagc gtcatcggca cggcgtcctg caactgcgta cggcccatct tcagcacgtc
     3901 ggcgaactcg gccgccttgg cctcgaaggc gccgcgcagc acgctcatcg ccgccgccag
     3961 ctcgccgatc atcacctgca gcgccacctt caccgccgtc gggtagacgt cgttggtgct
     4021 ctggctcatg ttgacgtgct cgatcgggtg gagatgcttg tagtcgccgc gctccagccc
     4081 gagatgctcc agcgcccggt tggcgatcac ctcgttggcg ttcatgttgg tcgaggtgcc
     4141 ggcgccgccc tggatcacgt cgaccgcgaa ctggtcgagc aacgccccct ggcgaatctc
     4201 ctcggaggcg cgcacgatgg cgtccgcctt cttcgcgtcg agcagcccga gatcgcgatt
     4261 cgccagcgcg gcggcctgtt tgacgctcgc cagcgcttca atcagctcgg ggtagaccga
     4321 aatcggcgtg cctgtgatcg gaaaattctc aaccgcacga acggtatgga cgccatagta
     4381 cgcctcggtc ggtacggcgc gatcgcccaa cagatcgtgc tcataacgca gcgcggaatc
     4441 aaccatgctc gaactccctc tgactatcct cgaacgcaca atcggtggct cagttccctc
     4501 ggtcgcccgt gagcacggat tggcgtcggt tcgcgaggca agggtatccc ttccgtttcc
     4561 atatacgcgc aggctctgcc acgacaacac atctgaagcg gatctaccct acccagacgc
     4621 ttatgaaaac ggtgctccgc aatgagcggc gtacgttgag atcgtggtcg gcagcccggg
     4681 acattctcgt tggtccccac ccgaaaacgg cggacctacc aactcaagta taccgagcgc
     4741 gcgacggagc ctgcccgaga gtcacagacg atcagccacg caaaaacgat tgacgagacg
     4801 cacgaccggt ggtatgaaca gaattcagca gtcacgggtt ggtgtcgatg cttcatgttg
     4861 ccggcgattg ccgggtgagg agtctccatg aaagaacgtc cccgcacacg cttctgggta
     4921 gaagcaggct tcgcgacgct cagtctcatc ttcgcggtac tcacgctcat ctggaaagac
     4981 tggatcgaga tcgtcttccg aatcgatccc gataaccaca gcggctcact ggagtggctg
     5041 atcgccttcg cggcgttggc cattgcgatc atcatcgccg gtgtggcgcg cgccgaatgg
     5101 cgccataccg ccagcgcgac agcctagcgt cgaccggccg gcgaccgcgt tgcctggtcg
     5161 ccggtcattt tccggggtgg tccgaccgtg atcgcgcacg accaccacat cggttcgtta
     5221 tcgaacgaaa gagaaa
//
LOCUS       DASUBI010000448         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1224740, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000448 DASUBI010000000
VERSION     DASUBI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224740"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="VFU72_08605"
     CDS             <1..2179
                     /locus_tag="VFU72_08605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5423585.1"
                     /translation="LEHVRLLQLERWQRDTLTTVHNLARRLNRAATYQTIAEIAVEEC
                     AAAFGCGKAALHRYEPHSGMLVMAAALGLPLPLVTALGHLTLDSGPFGRTTQERGLVV
                     TDDLWADESLEVLRELPESARLPLTAWALPLLDETDAPLGVITIFPPSRRSPNDTENK
                     LLHLLAHQIAVAMDRARLAERGHELYRATVASLAAAVDAKDPFTHNHSWQVAAYSRAI
                     AEAMALPPAEVEIIELAGLLHDVGKIGIPDRVLQKNDQLDADEWAMMRRHPELGAKIL
                     GDNPALASLMPLVRHHHERHDGHGYPDQLAGDQIPLGAAIVGLADAFDTMTSDRPYRR
                     SRDLAEAVAEVERSSGTHFHPEVVASFLAAVRSGTISVTPGMRRGTTRELRLKRVVGA
                     EARAFGLLQRISAEVSTLVEIDRFLLRLNELISAEFPESRCNVFVAQAESGNLVSIHD
                     PAGESVRQTLVCDIGVGIVGWVAQHGVPQNVRDVLEDARFRVTGHRLIRAELAVPLLV
                     EARCVGVIDLQHPEPGAFSPSDQQVLEMVATYVAQAIEVADLHHRIKQQAELDPLTGL
                     LNHRAFYRALDEAVEHARAASTQLALAILDLDNLKQINDTAGHLAGDSVIRHIAEILR
                     GRTRPEDTVARYGGDEFAIIMPRASRAVMEVRLRLVDEAIQSGYSDLVLSTVSWGIAC
                     YPEDGTRPAELVARADDAMYQVKSNRASRRLAATPSHAPTPTP"
     gene            2250..>2874
                     /locus_tag="VFU72_08610"
     CDS             2250..>2874
                     /locus_tag="VFU72_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5423586.1"
                     /translation="MTERDEATGRRAVTQPVLFEMGTDGRLVGNVGVLPELTTGSSLD
                     VARYWFRRYLEQSEHPKNTVNSYSYDLALFQLLIGPKAIDQVTRRDVAHFLDDSRTRS
                     TRKRRLTSVSGFFKFLIQHARVLRADPSAEFFPEHIPLKTPRPLFVDEQARLLAAAEA
                     DGPRAQAAIWLMLRLGLTRAEVLQLQADHIDRSLPERPVVFVYYDSRR"
BASE COUNT          450 a         1035 c          951 g          438 t
ORIGIN      
        1 gctggagcac gtacgcctgc tgcagctcga gcgctggcag cgcgacacgc tcaccacggt
       61 gcataacctg gcgcgacggc tcaaccgcgc ggcgacctat cagacgatcg ccgagatcgc
      121 ggtcgaggag tgcgcggccg ccttcggctg cggcaaggcg gcgctgcacc gctacgagcc
      181 gcacagcggc atgctggtga tggcggccgc gctcgggctg ccgctgcccc tggtcaccgc
      241 gctcggccac ctgacgctgg acagcggtcc cttcgggcgc acgacgcagg agcgcgggct
      301 ggtggtgacg gacgacctct gggcggacga gtcgctggag gtgctgcgcg agctgcccga
      361 gagcgcgcgc ctgccgctga cggcctgggc gctcccgctg ctggatgaga ccgacgcgcc
      421 gctcggtgtg atcacgatct tcccgcccag ccgccgcagc ccgaacgaca ccgagaacaa
      481 gctgctgcac ctcttggccc atcagatcgc ggtggcgatg gaccgggcgc ggctggccga
      541 acgcggccac gagctgtatc gcgccacggt cgcctcgctg gcggccgcgg tcgacgccaa
      601 ggatcccttc acccacaacc actcgtggca ggtcgccgcc tatagccggg cgatcgccga
      661 agcgatggcg ctgccgccgg ccgaggttga gatcatcgag ctggctggcc tgctgcacga
      721 tgtcggcaag atcggcatcc cagaccgggt gctgcagaag aacgaccagt tggacgccga
      781 cgagtgggcc atgatgcgcc gccaccccga gctgggcgcc aagatcctgg gcgacaaccc
      841 ggcgctggcc agcctcatgc cgctggtgcg ccaccaccac gagcgccacg acggtcacgg
      901 ctaccccgac cagttggccg gcgaccagat cccgctgggc gcggctatcg tcggcctggc
      961 cgacgccttc gacacgatga cctcggaccg gccctaccgc cggtcgcgcg acctcgccga
     1021 agcggtcgcc gaggtggagc gcagcagcgg cacccacttc caccccgaag tcgtggcgtc
     1081 ctttctcgcc gccgtgcgct ccggcacgat cagcgtgacg ccggggatgc gccggggcac
     1141 gacccgcgag ctgcgcctca agcgcgtggt cggcgccgaa gctcgtgcct tcggcctgct
     1201 gcagcgcatc agcgccgaag tcagcacgct ggtcgagatc gaccgcttcc tgctgcggct
     1261 caatgagctg atctccgccg agttcccgga gtcgcgctgc aacgtcttcg tggcccaggc
     1321 cgagagcggg aatctggttt cgatccacga cccggccggc gagtcggtgc gtcagacgct
     1381 ggtctgcgat atcggcgtgg gcatcgtcgg ctgggtcgcc cagcatggcg tgccgcagaa
     1441 cgtgcgcgac gtgctggagg atgcgcgctt ccgcgtcacc ggccaccggc taatccgcgc
     1501 cgagctggcg gtgccgctgc tggtcgaagc gcgctgtgtc ggcgtcatcg acctgcaaca
     1561 ccccgagccc ggcgccttct ctccttccga ccagcaggtg ctggagatgg tcgccaccta
     1621 cgtggcgcag gcgatcgaag tcgccgatct gcaccatcgc atcaagcagc aggccgaact
     1681 ggacccgctc accggtctgc tcaaccaccg tgctttctac cgcgcgctcg acgaagcggt
     1741 cgagcacgca cgcgcggcct cgacgcaact ggcgctggcg attctcgacc tggacaacct
     1801 caagcagatc aatgacaccg ccggtcacct ggccggtgat tcggtgatcc gtcacatcgc
     1861 cgagatcttg cgcgggcgaa cgcggcccga ggacaccgtg gcgcgctatg gcggcgacga
     1921 gttcgcgatc atcatgccgc gcgcctcccg cgccgttatg gaggtgcgcc tgcgcctggt
     1981 cgatgaagca attcagtccg gctatagcga cctcgtgctc agcaccgtct cctgggggat
     2041 cgcctgctat ccagaagacg gcacccgccc ggccgagttg gtggcacggg ccgatgacgc
     2101 catgtaccag gtcaagagca atcgcgcctc gcggcgcctc gccgccacgc cctcacacgc
     2161 acccacaccc acaccttgac aggcgaacgt tcgttcgcta ccgtacggtc agagtcgccg
     2221 acctgatcgg cacgagcagg aggctggccg tgacagagcg cgacgaggca acggggcgac
     2281 gtgcggtgac ccagccggtc ttgttcgaga tgggcaccga tgggcggctc gtcggcaatg
     2341 tcggcgtctt gccggagttg acgaccggtt cgagcctcga cgtggcgcgc tactggttcc
     2401 ggcgctacct ggagcaatcc gagcacccca agaacacggt caactcctat agctacgacc
     2461 tggcgctctt ccagctcctg atcggtccca aagcgatcga ccaggtgacg cggcgcgacg
     2521 tggcgcactt cctcgacgac agccgcacgc gctcgacgcg caagcgccgt ttgacctcgg
     2581 tctccggctt tttcaagttc ttgatccagc acgcccgggt gttgcgcgcc gatccgagcg
     2641 ccgagttctt ccccgagcac atcccgctca agacaccccg cccgctcttc gtcgatgagc
     2701 aagcacgtct gctcgcggcg gccgaggcgg acggaccccg cgcccaggcc gccatctggc
     2761 tgatgctgcg gctggggttg acgcgcgccg aggtgcttca gctccaggcg gatcacatcg
     2821 accgttcgct gcccgagcgg ccggtggtct tcgtctacta cgacagccgc cggc
//
LOCUS       DASUBI010000449         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1272320, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000449 DASUBI010000000
VERSION     DASUBI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272320"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFU72_08615"
     CDS             complement(<1..356)
                     /locus_tag="VFU72_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423587.1"
                     /translation="MKLRTWLRPGMFIKRWVIMLFGGLVLTSLALAMGLAWLYRNYHF
                     PTELAGIVRIVTLQFIPHPYREIGLLIVGAAWVFFSFYELSRSLLAPFLAQRADGQGL
                     VDIIQRHRFGPSAPELR"
     gene            589..1911
                     /gene="nusA"
                     /locus_tag="VFU72_08620"
     CDS             589..1911
                     /gene="nusA"
                     /locus_tag="VFU72_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5423588.1"
                     /translation="MKSDFYTAIAMIAQERGIPREAVLASVEQALATVYKKMSGIEGE
                     NVQVDLEPSAGTIRIYVLKDVVPEVEDEATQMTVSEARTTHPEAVIGDTVRFDRTPQN
                     FGRIAAQTAKQVVLQRIRDYEREGIYSEYIDRVGEVLNGIVQRADPKAVIVDLGKAEA
                     VMPAREQVPSERYRPGQRVKVYLLEVNKDQRGPQLIVSRTAPALIKRLFELEVPEIYS
                     GAVEIMAVAREPGLRSKVAVAARQEKVDPVGSCVGVRGVRIQNIVNELYGEKIDVIEW
                     SPDIPTFISNALSPAKPISVSLSEEDKVARVIVPTDQMSLAIGKDGQNARLAYKLTGW
                     RIDIKDPETLRNSGDDLLEQARAALAGVATDVMGFGRQPRLVRGDGTISVRGHDFGPL
                     SPDLVSMSVDVEIQDGVVSVFYERDLRARYDFETGRELPLTEEENSRV"
     gene            1960..2307
                     /locus_tag="VFU72_08625"
     CDS             1960..2307
                     /locus_tag="VFU72_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU5423589.1"
                     /translation="MTNEHGTRARRKGPRPKHVPLRTCVVCRRKEAKRGFVRIVRTPA
                     GAVTIDLSGKANGRGAYLCDDPACWRRAAEGTMLDRALKTELGHEAREELRHYAQDHF
                     RPDASEADAPAEP"
     gene            2324..4240
                     /gene="infB"
                     /locus_tag="VFU72_08630"
     CDS             2324..4240
                     /gene="infB"
                     /locus_tag="VFU72_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5423590.1"
                     /translation="MAQQRGRGGRGGRGGNSGGKGNRGRAATATFSGPRPISNEPRRA
                     PVVTGPIELGSVLTVGELADALGVSPVDVIKTLMARGVMANINQQIDYETASTVAESF
                     GVETEEHVPEIIQHANQEVEKRLGEVASDVNAVTRPPVVTIMGHVDHGKTKLLDAIRQ
                     TNVAEGEAGGITQHIGAYQVEIQGRRITFLDTPGHEAFTAMRARGAQVTDIAVLVVAA
                     DDGVMPQTREAVNHARVANVPIIVAINKIDLPAANPDRVKQQLSDIGVIPEEYGGEVP
                     FVEVSAREKLGIDDLLEMILLVADLMELKANPRQPAIGVIIEAKIDRNRGPVATALIQ
                     SGTLNLRDAVVVGSTYGRLRAMQDDRGRRVRRAEPAAPVEILGLLEVPDAGDILQVVA
                     DERVARQLAEERAARRRAESFNETRVIKLDEIFNQVEHGGTAELRIVLKADVQGSLEA
                     IQGALVKLNEQAESVGIKVVHTGTGAISESDVMLAVAAGAIILGFSVRPDAAAKRAAD
                     SAGVDVRYYDIIYQLIDDIKAAMVGLLAPETREISDGYAEVRETFRLPTREVVAGLYV
                     LDGKAVRNDRVRVLRSGTVVHDGEVSSLKRFKEDAREVQSGYECGLVVAGFTDVQVGD
                     QIEFYHTEQVARTL"
BASE COUNT          825 a         1416 c         1490 g          665 t
ORIGIN      
        1 cgcagctccg gcgcgctggg gccgaaacgg tgccgctgga tgatatcgac cagcccctgg
       61 ccatcggcac gctgggccag aaatggcgcc agcagcgagc ggctaagctc gtagaagctg
      121 aagaagaccc aggccgcgcc gacgatcaag aggccgatct cgcgatacgg atgcgggatg
      181 aactgcagcg tgacgatgcg gacgatgccg gccaactcgg tggggaaatg gtagttgcga
      241 tagagccagg ctaaccccat cgccagcgcc aggctggtta gcaccagtcc accgaagagc
      301 atgatgaccc agcgcttgat gaacatgccc ggacggagcc atgttcggag cttcattgat
      361 cccctcaacc cctcccgaag gcggtcgcgc gcccgattat agcaccgccg ggcgcgcgct
      421 ctgcttggct gggccagccg gactctgcta taattcctac ggaaccgacc gcgcccggga
      481 caagtgggct ccgcccactt tcttttttgc ccggcggtac cccatcctgg caactcgcgc
      541 ggcgcatccc gcgccccgac gggtcgacaa aggaggtctc ctagaacaat gaaaagcgat
      601 ttctataccg cgatcgcaat gatcgcacag gagcgcggta tcccacgcga agccgtgctg
      661 gcctccgtcg agcaggcact ggctaccgtc tacaagaaga tgtcggggat cgagggcgag
      721 aacgtccagg ttgacctgga gccgagcgcc ggcaccattc gcatctacgt cttgaaggac
      781 gtggtccccg aagtcgagga cgaagccacc cagatgacgg tctccgaggc gcgcacgacc
      841 catcccgaag cggtcatcgg ggatacggtc cgcttcgatc gcacgccgca gaacttcggc
      901 cgcatcgcgg cgcaaacggc caagcaggtc gtcctgcagc gtattcgcga ctatgagcgc
      961 gagggcatct acagcgagta catcgaccgc gtgggggagg tgctcaacgg catcgtgcaa
     1021 cgggcggatc ccaaggcggt catcgtcgat ctgggcaagg ccgaggcggt catgcccgcc
     1081 cgtgagcagg tgccttccga gcgctaccgg cccggccagc gtgtcaaggt ctacctgctg
     1141 gaggtcaata aggaccagcg tgggccgcag ctgatcgtct cacgtaccgc gccggctttg
     1201 atcaagcggc tcttcgagct agaggtgcct gagatctaca gcggtgctgt cgagatcatg
     1261 gcggtagcgc gtgaaccggg actgcgttcg aaggtcgcgg tggccgcccg gcaggagaag
     1321 gtcgatccgg tcggctcctg cgtcggcgtg cgcggtgtgc gcatccagaa catcgtcaac
     1381 gagctgtatg gcgagaagat cgatgtgatt gagtggtcgc ccgacatccc gaccttcatc
     1441 agcaacgcct tgagcccggc caagccgatc agtgtctcgc tgagcgagga ggacaaggtc
     1501 gcccgggtca tcgtgccgac cgaccagatg tcgctggcga tcggcaagga cggccagaac
     1561 gcgcgtctgg cctacaagct caccggttgg cgcatcgata tcaaggatcc cgagacgcta
     1621 cgcaacagcg gagacgatct actggagcag gcgcgcgccg cgctggccgg cgtcgcgacc
     1681 gacgtgatgg gcttcggccg gcaaccgcgc ctggtgcggg gtgacggcac gatcagcgtg
     1741 cgtgggcacg atttcggccc gctctcgccg gatctggtca gcatgagcgt cgacgtcgag
     1801 atccaggacg gcgtggtcag tgtcttctac gagcgcgatc tgcgcgcgcg gtacgacttc
     1861 gagaccggcc gagaactccc cctgaccgaa gaggagaatt cccgggtcta acgcgtgcgg
     1921 cccggaacgt ggacgcaccg gacctgggag caggagacga tgaccaatga gcacggaacg
     1981 cgggctcggc gcaaggggcc acgccccaag catgtcccgc tgagaacctg tgtcgtctgc
     2041 cgacgaaaag aggcaaagcg cggcttcgtg cgaatcgtgc gcacgcctgc aggcgcggtc
     2101 acgatcgacc tgagcggcaa agccaacggg cggggcgctt atctctgtga cgatcccgcc
     2161 tgctggcgac gcgccgccga gggcacgatg ctcgatcgcg cgctcaagac ggagttgggc
     2221 cacgaggctc gcgaagagct ccgccactac gcgcaagacc atttccgccc agacgccagc
     2281 gaagcggacg cgcccgccga gccctaaacc gaggaggtac tacatggccc aacaacgagg
     2341 acgtggcggt cgcggcggcc gtggcggcaa tagcggcggc aaggggaatc gcggtcgagc
     2401 cgccaccgcg acctttagcg gtccccggcc gatctccaac gagccgcggc gcgcgccggt
     2461 cgtcaccggc ccaatcgagc ttggctcggt tctgaccgtc ggcgaactgg ccgacgcgct
     2521 cggcgtcagc ccggtcgacg tcatcaagac cctgatggcc cgcggcgtca tggcgaacat
     2581 caatcaacag atcgattatg agacggcttc taccgtagct gagtcttttg gggtcgagac
     2641 cgaggagcat gtccccgaga tcatccagca tgccaatcaa gaggttgaga agcggctggg
     2701 cgaggtcgcc agcgacgtca acgccgtgac tcgcccgccg gtggtgacca tcatggggca
     2761 cgtcgaccac ggcaagacca agctgctgga cgccatccgg cagaccaacg tggccgaggg
     2821 ggaggccggc ggcatcaccc agcacatcgg cgcctatcag gtcgagatcc agggtcgcag
     2881 gatcaccttt ctggacacac cgggccacga ggcgttcacg gcgatgcggg cgcgtggcgc
     2941 gcaggtgact gacattgccg tcctggtggt cgcggccgac gacggcgtga tgccccagac
     3001 gcgcgaggcg gtcaaccacg cccgcgtcgc caacgtgccg atcatcgtgg ccatcaacaa
     3061 gatcgacctg ccggccgcca acccggaccg ggtcaagcag cagctctccg acatcggtgt
     3121 gatcccggag gagtacggcg gcgaggtgcc gttcgtcgag gtgtcggcgc gcgagaagct
     3181 gggtatcgac gacttgctcg agatgatcct gctggtcgcg gacctgatgg agctgaaggc
     3241 caacccccgg cagccggcga tcggcgtgat catcgaagcc aagatcgacc gcaaccgcgg
     3301 cccggtcgcc accgcgctga tccagtccgg cacgttgaat ctgcgtgacg cggtcgtggt
     3361 cggctcgacg tacggccgcc tgcgcgcgat gcaggatgac cgcgggcggc gcgtgcgacg
     3421 cgccgagccg gccgcgccgg tcgagatcct cggcctgctg gaggttccgg atgccggcga
     3481 catcctgcag gtggtcgcgg acgagcgggt ggcacgccag ctggcggagg agcgcgcggc
     3541 ccggcggcgg gcggagagct tcaacgagac acgcgtcatc aagttggatg agatcttcaa
     3601 tcaggtcgag cacggcggca ccgccgagtt gcgtatcgtg ctcaaggccg acgtgcaggg
     3661 ctctttggag gcgatccagg gcgcgctggt caagttgaac gagcaggccg aatcggtcgg
     3721 catcaaggtg gtccacaccg gcaccggcgc catctccgag tccgatgtca tgctggccgt
     3781 cgcggccggc gcgattatcc tgggcttcag tgtccggccg gacgcggccg ccaagcgcgc
     3841 cgccgattcc gcgggggtcg acgtccgcta ctatgacatc atctatcagc tgatcgacga
     3901 tatcaaagcc gccatggttg gcctgctggc accggagacg cgtgagatct ccgacggcta
     3961 cgccgaggtt cgagagacct tccggctacc cacgcgcgag gtcgtcgccg ggctctacgt
     4021 cctcgacggc aaggccgtcc gcaacgaccg cgtgcgcgtg ctgcgcagcg gcaccgtggt
     4081 ccacgacggc gaagtctcct cgctcaagcg tttcaaggag gacgctcgcg aggtgcagag
     4141 cggctatgaa tgcggtctgg tcgtggccgg cttcaccgat gtccaggttg gggatcagat
     4201 cgagttctac cataccgagc aggtggctcg aacgttgtag ccggcgttgg ggcggcctgt
     4261 ccgccccaac ctaccccctc cctacgcccg ctacccctgc cggcgacgaa gggagctgag
     4321 accaatgacc acgcgacgac agcagcaggt cggcgagttc atccgcgaag aagtcagcga
     4381 gatcatccag cgcgag
//
LOCUS       DASUBI010000450         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_128356, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000450 DASUBI010000000
VERSION     DASUBI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128356"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(557..796)
                     /locus_tag="VFU72_08635"
     CDS             complement(557..796)
                     /locus_tag="VFU72_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423591.1"
                     /translation="MLEYRRASLTHALHDKGYQRLQIRRFRVFGQVAWQVDLFDRAGT
                     VIGVLGGPAVRFTTVLAQIGALPPTAASDGAGSPR"
     gene            complement(1558..>2073)
                     /locus_tag="VFU72_08640"
     CDS             complement(1558..>2073)
                     /locus_tag="VFU72_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423592.1"
                     /translation="ATIARDLRRLGVLLRQRLGTDIAADRLAIDEGFLRVLRQAWTSF
                     AAAPKGSRSASEHLAVITRVHEARAKLLGVSAADEARLRKATAEADGGIGAFLASLAV
                     FEEQERIRYLATATDEDLVPHARDTNDLVGDLLRRFGKLPATAGEPAPAQEVEEAEED
                     EPAPPTDVPVW"
BASE COUNT          285 a          753 c          705 g          330 t
ORIGIN      
        1 agccggtggc ggcgacaaac tcatcgagga tctgcgactt ctgaatcggg ctcgccgcct
       61 ggtagcggcc ggcgatctgg gccagcaact cgcgcttcgc ctggaaactc atccgcgacc
      121 tcacgtcctc cccctccgct cgtggccaac cagcgtcggg tgcgattatt cgtgaggcaa
      181 gtcccgccgc aaagtgcact catccgtgac gcaatgcgct catccgtgac gcaatgcgcc
      241 ggcagatcta attgtcgttg aacactgcgt cgttcagcgc gatcagcccc gcctgcgcta
      301 tttcggtgat gactgccgcg ctggccgtgt cgtcgcagcc ggggtggccg cccttccagg
      361 cacgctgagg cggttggggt gtgggcgcat cctggatagg aaagcaggtg gggcaatggg
      421 cgcggcaggc tgacgacgtg tcaaacctac cgactgccgc cgtgacgagc cgcactccag
      481 tcgccgccac gcccgccggc ccgcctcccc gcgaccggga cagcgcgact tccagcgttg
      541 ctgggcgccg gcgccgtcac cgcggcgagc cggcgccatc gctggccgcg gtcggcggta
      601 gcgcgccgat ctgtgccagc accgtcgtaa acctcacggc agggccgccg aggacgccga
      661 tgaccgtgcc cgcccgatcg aagaggtcga cctgccaggc gacctgcccg aacacacgga
      721 acctccggat ctgcaagcgc tgataccctt tgtcatgcag ggcatgggtg agactggcgc
      781 gccggtactc cagcatggcg acatcgctcc ttggtgggcg gaccggcgcc tcgctggccc
      841 cgcccgcgcg gctggcgcgt tcgtgccacg ggccacgggc ctggcgctgt gaaacggaca
      901 gcgcccgtca gcatagcagg tagcgacggt gccgcatgcg accatcctcc tggtccgtgc
      961 gcccgaccgc gtgctgccag taccacccgc atcgagcacg ccgcatgaat gcgtctgcca
     1021 gctctcgtcg cgcgtggcgt ccacgctatt cacaccgggc gcctgcgccg caccaccgcc
     1081 tcgggcacgc cttgctgtag ttcgcgtgcc ccaggggatt cgccttgcgc tcaggcggtc
     1141 ggcgctggtc ggcgcgcgac tcggcatggc ctcgctgaac ctggacgact ggttcgcgcc
     1201 cttctacgct gacgctatct gcccgctcca tccctttctg gcagccgatc ccctggcgcc
     1261 agcggacgaa taatcattgg ccctctcacc agccgccgcc accaccgcgg ctgaagccgg
     1321 gaggttcttg cgcggcctga ccggcacgat ggtcggtgcg gcacaccgtt gacgtcgagg
     1381 tcgagcgggg gctggcccgc cagcgcggtg gcgtgtcgca cccccattgt cgcagccggc
     1441 cagccgggcc acgctcccgt ttttgtcgcc agcggtgccg agaccaccct gcgccaggct
     1501 gcgacgcgct gcagcctggc gcaggggagc tcgacccggc gctggcggcc ggtagggcta
     1561 ccagaccgga acgtccgtcg gtggcgccgg ctcgtcctct tccgcctcct ccacctcctg
     1621 tgctggcgcc ggctcgcccg cggtcgcggg gagcttgcca aaccggcgca agaggtcgcc
     1681 gaccaggtcg ttggtgtccc gggcgtgggg caccagatct tcatcggtgg cggtggcgag
     1741 atagcggatc cgttcctgtt cctcgaagac ggcgaggctg gcgaggaagg cgccgatccc
     1801 gccgtccgcc tcggccgtcg ccttgcgcag cctggcttcg tcggcggcgc tgacaccgag
     1861 cagtttggcg cgggcttcgt ggacccgcgt gatgaccgcc aggtgctcgc tggcgctccg
     1921 gctgcctttg ggcgccgcgg caaaactcgt ccaggcctgg cgcaggacac gcaagaagcc
     1981 ctcatcgatg gccaggcggt cggcggcgat gtcggtgccc aggcgctgcc gcagcaggac
     2041 gccgagccgc cggaggtcac gcgcaatcgt ggc
//
LOCUS       DASUBI010000451         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_445163, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000451 DASUBI010000000
VERSION     DASUBI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445163"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFU72_08645"
     CDS             complement(<1..501)
                     /locus_tag="VFU72_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423593.1"
                     /translation="MVSDQWGLVECACGWAGPGDPLESARGLSRIITRIDRRLATRAA
                     RADLARLGSGRWWPGQLGVFYTLALFLGSTLVYAVIAAVLAGLAVLTISLVRDQAWVG
                     AGIVAFLFVVFSLSLLLDRTQAEGIQAPRERFPRLWDALDDVRVRTGAPLPHRVVLVP
                     EAAAYVF"
     gene            complement(723..1028)
                     /locus_tag="VFU72_08650"
     CDS             complement(723..1028)
                     /locus_tag="VFU72_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423594.1"
                     /translation="MEITLMIIALVLALPIGLALSALMSTARARWLALLAGIVGDLVV
                     AVAIYAYVTVAKVTIDALTYFLGALFACTMGVMAFALIANFVVGLLGRRPEVSAPEY"
     gene            complement(1247..>2285)
                     /locus_tag="VFU72_08655"
     CDS             complement(1247..>2285)
                     /locus_tag="VFU72_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5423595.1"
                     /translation="ERWQAEGQRVALATVITVTGSAPRGGGAVLAASEAGDIAGSVSG
                     GCVEPAVIEEGMRAIRTGRPKLLQFGITEEQNVERIGLSCGGEIRVFVQRLPDVAPLA
                     AELRAERPVAVATLVSAPDANAAQPGAQLLVREGQPPEGSLGTPALDAAAVTSARELL
                     REDAPTLRSLPLAEESPEAEADVFFAVYPAPPSLIIVGAGHISIPLSRIAKVLGYRVT
                     VLDPREAFNTEERLPDADERLLEWPDEALPRLPLSSATAVAVLTHDEKFDVPALAAVL
                     QHPVGYVGAIGSRGTRERRDRRLREAGVTDEQIARIHGPIGLNIGARTPEEIGLAIMA
                     QIVAVRNGSAA"
BASE COUNT          385 a          774 c          833 g          293 t
ORIGIN      
        1 gaagacgtac gcggctgcct cgggaacgag cacgacgcga tgcggcagcg gcgcgcccgt
       61 gcgcacgcga acgtcgtcga gcgcgtccca caggcgcggg aagcgctcgc gcggtgcctg
      121 gataccttca gcttgggtgc ggtcgagcaa caacgaaagg ctgaagacga caaagaggaa
      181 ggcgacgatg ccggcgccaa cccatgcctg atcgcgaacc agactgatgg tgaggacggc
      241 gagcccggcg agcacagcgg caatcacggc gtagaccagc gtgctgccca ggaagagcgc
      301 cagcgtgtag aagacgccga gctgccctgg ccaccagcgt ccgctgccca ggcgggcgag
      361 atcggcacgt gccgcgcgtg tggcgagccg ccgatcaatc cgcgtgatga ttcggctgag
      421 gccgcgcgcg gactccagcg gatcgccggg tccggcccag ccgcaggcgc attcgacaag
      481 gccccactgg tcggaaacca caggtccgct acagagcgga caacagagcg cgacagcggt
      541 tgcgacaagc ggctgcatcc tcaccctccc ccacaacggc atccgagcgc cggatggagc
      601 gccagcacgc tcagagaatc acgccgcgac acatgctgag catgcgttca gtatagagcg
      661 cgtgtctagt gccggccgat gcgcgagaga aggagggtgg gtacgggtgg agaggaggga
      721 ggctagtatt cgggggcgga gacctcgggc cggcggccga gcagcccgac gacgaagttg
      781 gcgatgaggg cgaaggccat cacgcccatc gtgcaggcaa agagcgcgcc gaggaagtac
      841 gtcagcgcgt caatggtgac cttggcgacc gtgacatagg cgtagatcgc caccgcgacc
      901 accagatcgc cgacgatacc ggccagcagc gccagccagc gggcgcgcgc ggtgctcatc
      961 agtgctgaga gcgccaggcc gattggcagc gccagcacca gcgcgatgat catcaacgta
     1021 atctccatgc gggcgactca tttctcgtga tgggggatga tgatgggtgg ggatgagcgt
     1081 gccggatgcg ccggcgctcg cctcgattgt agcaaggtcg cgccggagac gccagcggtc
     1141 aggccatgcg ggcattgcgg cgggcgctca ggggccaacg ccacgcctgt acgcctcgcc
     1201 ggtcgcgtcc aatcgcccga tgcgggttgg cggccggcga ttacgactag gctgcgctcc
     1261 cattgcgcac ggcgacgatc tgcgccatga tcgccagccc gatctcctct ggcgtgcggg
     1321 cgccgatatt gagaccgatg gggccgtgga tgcgcgcgat ctgctcgtcc gtcacgccgg
     1381 cctcgcgcag ccgccggtcg cgccgctcgc gggtgccgcg gctgccgatc gcgccgacgt
     1441 agccgacggg atgctggagc acggcggcca gcgcgggcac gtcgaacttc tcgtcatgcg
     1501 tcagcacggc cacggccgtc gcgctgctga gcggcagccg cggcagcgcc tcgtcgggcc
     1561 attcgagcag ccgctcgtcg gcgtcgggca ggcgctcctc cgtattgaag gcctcgcgcg
     1621 ggtcgagcac ggtgacgcgg tagccgagca ccttggcgat acggctcagc ggaatcgaga
     1681 tgtggccggc gccgacgatg atgagcgagg gcggcgcggg atagaccgcg aagaagacat
     1741 cggcctcggc ttcggggctt tcctcggcga gcggcagcga gcgcaacgtg ggcgcatcct
     1801 cgcgcagcag ctcgcgcgcg ctggtgacgg ccgccgcgtc gagcgccggc gtgccaagac
     1861 tgccctccgg cggctgcccc tcgcggacga gcaactgcgc gccgggctgg gctgcgttcg
     1921 catccggcgc gctgaccagc gtggcgacgg cgacgggacg ctcggcccgc agctcggccg
     1981 ccagcggcgc gacatcgggc agccgctgga cgaagacgcg gatctcgccg ccgcacgaaa
     2041 ggccaatgcg ctcgacattc tgctcctcgg tgatgccgaa ctgcagcagc ttcggccgcc
     2101 cagtgcggat cgcgcgcatg ccctcctcga tcaccgccgg ctcgacacag ccgccgctga
     2161 cggagccggc gatatcgccg gcttcgctgg ccgccagcac ggcaccgccg ccgcgcgggg
     2221 ccgagccggt gacggtgatg acggtcgcca gcgcgacgcg ctggccctcc gcctgccagc
     2281 gttcg
//
LOCUS       DASUBI010000452         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1237102, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000452 DASUBI010000000
VERSION     DASUBI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237102"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="aroF"
                     /locus_tag="VFU72_08660"
     CDS             <1..826
                     /gene="aroF"
                     /locus_tag="VFU72_08660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5423596.1"
                     /translation="WDFHPQKTVFDVGDVTIGGEEVVVIAGPCSVESEEQTVATAKAV
                     KAAGASMLRGGAFKPRTSPYEFRGLFEEGLRLLAQARAETGLPVVTEVLSAHDVELVA
                     EYADVLQIGARNMQNFQLLEAVGKTDKPVLLKRGMSAQLEEWLLAAEYVMAQGNEQVI
                     LCERGIRTYETLTRNTLDLSAVPVVKRLSHLPVIVDPSHGTGHWYLVPAMMLAAVAGG
                     ADGLMVEVHPNPDNAMSDGAQSLTRENFAVAMARVGAVAEAVGRRVPEAGRAPVAG"
     gene            991..1659
                     /gene="hisIE"
                     /locus_tag="VFU72_08665"
     CDS             991..1659
                     /gene="hisIE"
                     /locus_tag="VFU72_08665"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU5423597.1"
                     /translation="MNSDAVEPLLDEVRFDAQGLVPAVVQDAVSGQVRMLGYMNREAL
                     RRTIATGRLTFWSRSRGQLWQKGETSGNVHEVVEVRADCDGDALLVRVNPLGPTCHTG
                     AESCFDGQPLASLRAEQFGSGVVDQVARVVAERQASPVEGSYTSYLLTEGIDKIGKKI
                     GEEAAEVIIAAKNGEPAGIANESADLIYHLLVLLEASGVAPGRVWDVLSTRRGRPAPD
                     HSSR"
     gene            complement(1644..2168)
                     /gene="rimM"
                     /locus_tag="VFU72_08670"
     CDS             complement(1644..2168)
                     /gene="rimM"
                     /locus_tag="VFU72_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872347.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5423598.1"
                     /translation="MTEQAAAGADRLSIGVIVGPQGIKGQLKMQLWTHFPERIPTLSE
                     IFLNDEAAPRRLEHAYLSGGLAILTVAGVDTREAAEELRGSIVRIPASEAAPLAEDEY
                     FHFQLIGLPVFDEAGTPLGELAEILETGANVVYLVRDQQGRDLLLPALKSVVLDIDLK
                     RGRIIVRPPAYLEE"
     gene            complement(2161..2400)
                     /locus_tag="VFU72_08675"
     CDS             complement(2161..2400)
                     /locus_tag="VFU72_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU5423599.1"
                     /translation="MEELKSLVEFMACNLVENPGDVRVEAQQRGRVVSIQLMVPADEM
                     GKVIGRQGRVARAMRTLLTIAASRHALRASLDIND"
     gene            complement(2429..2722)
                     /gene="rpsP"
                     /locus_tag="VFU72_08680"
     CDS             complement(2429..2722)
                     /gene="rpsP"
                     /locus_tag="VFU72_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5423600.1"
                     /translation="MIKLRLRRMGKKRQPSYRIVAAEARMPRDGRFIEIIGFYNPRTE
                     PSEMRVDAERARYWLEHGAQPTDTVRRILQTTGVLPGGKPAEGQSAVSEAVTE"
     gene            complement(3312..>3764)
                     /locus_tag="VFU72_08685"
     CDS             complement(3312..>3764)
                     /locus_tag="VFU72_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423601.1"
                     /translation="PSTILVEEYDEQSNEEYEGGFTPSPRRFPPRTPSAGYDPNPIYK
                     TEPADQRYSLVEETRQMAPQSSGRLALANGGIARAGDEPQVIVASPDQLGGKEAGGEA
                     EVEAQATRQMPRIEPDRRDEALRDGICPACHADIRSAEVYCPNCGRIL"
BASE COUNT          565 a         1174 c         1418 g          607 t
ORIGIN      
        1 ttgggatttc cacccgcaga agacggtctt cgacgtcgga gacgtgacga tcggcggcga
       61 ggaggtcgtc gtcatcgccg gcccctgctc agtcgagagc gaggagcaga cggtcgcgac
      121 ggcgaaggcg gtcaaggcgg ccggcgcctc gatgctgcgc ggcggcgcct tcaagccgcg
      181 cacctcgccc tacgagttcc gcggcttgtt cgaggagggg ctgcggctgc tggcgcaggc
      241 gcgcgccgag accgggctgc cggtggtgac cgaggtgctg agcgcccacg acgtcgaact
      301 ggttgccgag tacgccgacg tcctgcagat cggcgcgcgc aacatgcaga acttccagct
      361 cctggaggcg gtcggtaaga ccgacaagcc ggtgctgctc aagcgcggca tgtcggcgca
      421 gctggaggag tggctgctgg cggccgagta cgtgatggcc caggggaacg aacaggtcat
      481 cctctgcgag cgcggcattc gcacctacga gacgctgacg cgcaacacgc tggatctgag
      541 cgcggtgccg gtggtcaagc ggctgagcca cctgcccgtg atcgtcgatc ccagccacgg
      601 caccgggcac tggtacctgg tgccggcgat gatgctggcg gcggtcgcgg gcggcgcgga
      661 cgggctgatg gtcgaggtgc accccaaccc ggacaacgcc atgtcggacg gggcgcagtc
      721 gctgacccgc gagaacttcg cggtggcgat ggcgcgggtc ggcgcggtgg ccgaggcggt
      781 cggccggcgc gtgccggagg cagggcgagc gcctgttgcg gggtagtggc ttggcccgcg
      841 ggcagcatgg gtgccgggtg aggacggatc cttcgacagg ctcaggatga cagggtgaat
      901 agggggcgga cgacgaggct ctgtgccgga gcgcggctaa aggatccgtt cctcccctag
      961 cgcctcgttt gagcgaagtg taggagcgac atgaactccg acgcggttga gccgttactt
     1021 gatgaggtcc gtttcgacgc gcaggggctg gtgccggcgg tggtgcagga cgcggtgagc
     1081 ggtcaggtgc ggatgctcgg ctacatgaac cgtgaggcgc tgcggcggac gatcgccact
     1141 gggcggctga ctttctggag ccgcagccgc gggcagctct ggcagaaggg cgagacctcg
     1201 gggaacgtgc atgaggtggt cgaggtgcgc gccgactgcg acggcgacgc gctgctggta
     1261 cgggtcaacc cgctcggccc aacctgccac acgggcgccg agagctgctt cgacggccag
     1321 ccgctggcgt cgctgcgcgc ggagcagttc ggcagcgggg tggtcgatca ggtggcgcgc
     1381 gtggtggccg agcgacaggc cagtccggtc gaaggctcct acaccagtta cttgttgacc
     1441 gagggtatcg acaagatcgg caagaagatc ggcgaggagg cggccgaggt gatcatcgcc
     1501 gctaagaacg gcgagccggc gggaatagcc aacgagtcgg ccgacctgat ctaccacctg
     1561 ctggtgctgc tggaggcgag cggcgtcgcg cccgggcggg tctgggacgt gctcagcacg
     1621 cggcggggac ggcccgcgcc ggatcactct tcaaggtagg ctggtgggcg cacgataatg
     1681 cggccgcgct ttaggtcgat atcgagtacg acgctcttta gtgccggtag cagcagatcg
     1741 cgtccctgct ggtcgcggac caggtagacg acgttagcgc cggtttccag gatctcggcc
     1801 agttccccca gcggcgtgcc ggcctcgtcg aagacgggca gcccgatcaa ctgaaagtgg
     1861 aaatactcgt cctcggccag cggcgccgcc tccgacgcgg ggatgcgcac gatggagccg
     1921 cgcagttcct cggcggcctc gcgggtatcg actccggcga cggtgaggat ggcgagcccg
     1981 ccgctgaggt aggcgtgctc caggcggcgc ggggcggctt cgtcgttgag gaagatctcg
     2041 ctaagcgtgg ggatacgctc ggggaagtgg gtccagagct gcatcttgag ctggcccttg
     2101 atcccctgtg ggccgacgat gacgccgatc gagaggcggt cggcgccggc ggccgcttgc
     2161 tcagtcattg atatccaggc tggcgcgcag ggcgtggcgc gaggcggcga tggtgagcag
     2221 ggtgcgcatg gcgcgggcga cgcggccctg ccggccgatg accttcccca tctcgtcggc
     2281 cggcaccatg agctggatgc tgacgacgcg gccacgctgc tgcgcctcga cacgcacgtc
     2341 gccggggttc tcgaccagat tgcaggccat gaattcgacg agcgacttca gctcttccat
     2401 cggcatctcc tcctgctggc cggctgcgtt actcggtgac ggcctcgctg accgcgcttt
     2461 gaccctcggc cggcttgccg ccgggcagga cgccggtggt ctgcaggatc cggcgcacgg
     2521 tgtcggtcgg ctgggcgccg tgctcgagcc agtagcgggc acgttcggcg tcgacgcgca
     2581 tctcagaggg ttcggtgcgc gggttgtaga agccgatgat ctcgatgaag cggccgtcgc
     2641 gcggcatgcg cgcctcggcg gcgacgatgc ggtagctcgg ctggcgcttt ttccccatgc
     2701 gccgcaatcg caacttgatc acgctggtgt cggtcctttc ctgatctgat tccgcgctcg
     2761 ccaactcggg ttcgccgtga gggcgcgagc gttgcgcgtc agaaggtgag catccgtccg
     2821 gccgctcgcg cggcacgctg gcaggcatac agcaatcgcc cggggcggaa cccgggcgag
     2881 catgactata gcacgcggct gcctgtgggt caaacgcgcc ctcacgaagt ggagcctcgg
     2941 ctcctccctg cccgggggca atgctgggcg cggcgaggag tcggctgggg atacgtggca
     3001 gacgacgatg acctgcctcc tcttccgccc agtattctcc cagtcatccc gagcggaatc
     3061 gagggatccg acctggtgcg gctatcaata ccaccgttgg ccacactccg tcgcccacgc
     3121 cggcgcgccg tggaggggaa tggctgtcgc gcggcgtggc tgccgggcga ggacggatcc
     3181 ttcgacaagc tcaggatgac agggaaaggg agggagtctt ccggacgagg ttgagcgtgc
     3241 tggtacgtcg cgctctcagc cagtctcccg tgctcccgtc gccagaaggg gcgaagcgac
     3301 gcggacgcgg tctagaggat gcggccgcag ttggggcagt agacctcagc ggagcggatg
     3361 tcggcgtggc aggcggggca gatgccgtcg cgcagggcct catcgcggcg gtccggctcg
     3421 atgcgcggca tctgacgggt ggcctgcgcc tccacctcgg cttcgccacc agcctctttg
     3481 ccgccgagct gatccgggct ggcgacgatc acctggggct cgtctccggc gcgggcgatg
     3541 ccgccattgg cgagagccag ccggcccgac gactgcggcg ccatctgccg cgtctcctcg
     3601 accagcgagt agcgctggtc cgccggctcg gtcttgtaga tcggattggg gtcgtagccg
     3661 gcgctcggcg tgcgcggcgg gaagcgacgc ggggacggcg tgaagccgcc ttcgtactct
     3721 tcattggact gctcatcata ctcctcgacc agaatcgtgc tggg
//
LOCUS       DASUBI010000453         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1052498, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000453 DASUBI010000000
VERSION     DASUBI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052498"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="VFU72_08690"
     CDS             <1..2100
                     /locus_tag="VFU72_08690"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU5423602.1"
                     /translation="AVGSAGLITLAYYRVKPVYEAVAQFRVEPTEDIFNTKNFNQATF
                     AEYRTSQVTLVTSPNVLSDALYQHPDLFHYPLLQDAENAQDAEGVIRRAIQVAPVRET
                     NQIQVSMTSTSAEEAAAFVNAVVTAYIDYAKGVRDRDAKLRRQSLESVEQEAKTEVDH
                     KRAALQQLRDKLGNEVLLEAKDRNQISAQDYALLNQELVKVRIERIGVQGQLDILRGE
                     KPAGARGSDSHALEQAINDAFYADPVVREVVAKRLALETQLVKTKTMRNPGSDPFTRH
                     VRGKIKAENARLDQLWQAMEPKLRRQLASGGGAADGDHIVAATERQLASLTAQEQALE
                     DRLDQMTLKNDRAGNDALKMDFARIDLQRAEAVLSQVQTHLRQLDFESRNALARIQLD
                     FAARPMLRPSSNHRLHAMLAAPLAMWFAVLGLLGLIELKSGRVADPEELSSRSRLQVL
                     GVVPPLPRIRPSAGQLALGSDGRAQRQLEEFVQSLDHLRVALCARPDPWGRNRHCVLI
                     TSACGSEGKTTLAAQLAERCVNAGLMTLLIDADLRNPTLSRMLDAEQEAGLINVLRGE
                     RAPEDAIIVVEDAGGFHFLPAGTPRVDPSRLLQGDRLGNLLAQARESFEMIIVDAPPV
                     LPVPDALTIGRWTDGAVLAVRYDNSRFPLVDRANRRLAHVGVPVIGAVVNGVRSPDGA
                     YGGYYAYGGSSSAEPAV"
     gene            2134..>2604
                     /locus_tag="VFU72_08695"
     CDS             2134..>2604
                     /locus_tag="VFU72_08695"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HEU5423603.1"
                     /translation="MKSRRLSNFSLLTSSQGTQEPPSTMTTLPINSPTARPSWAGSAL
                     VVVVVTAALVASFAPCFVNLVGLWDADPNNSYGYLVIPMAAYILWDRRRLLVPQSLAP
                     CWWGFAPLAALLALRVLLYERNEQYMEAALIPWVVGCLVLILGGWHLLRVALPAV"
BASE COUNT          425 a          942 c          865 g          372 t
ORIGIN      
        1 gcggtcggtt ccgcgggcct gatcacgctg gcctactacc gcgtcaagcc ggtgtacgag
       61 gccgtcgcgc agttccgcgt cgagccgacc gaggacatct tcaacaccaa gaacttcaac
      121 caggccacct tcgccgagta ccgcaccagc caggtcacac ttgtcactag tccgaacgtc
      181 ctgagcgatg cgctctacca gcacccggac ctgttccact acccactgct gcaagacgcc
      241 gagaacgccc aggacgccga gggcgtgatc cgccgcgcga tccaggtcgc gccagtgcgc
      301 gagacgaacc agatccaggt ctcgatgacc tccacatcgg cggaggaggc ggccgcgttc
      361 gtcaacgccg tcgtcaccgc ctacatcgac tacgccaagg gcgtgcgcga tcgcgacgcc
      421 aagctgcgcc ggcagagcct cgaaagcgtc gaacaggagg ccaagaccga ggtcgaccac
      481 aagcgggccg cgttgcagca actgcgcgac aagctcggca acgaggtcct gctcgaggcg
      541 aaggaccgca accagatctc ggcgcaggac tacgccctgt tgaatcagga gttggtcaag
      601 gtgcgcatcg agcgcatcgg cgtgcagggg cagctcgaca tcctccgggg cgagaagccc
      661 gccggcgcgc gcggcagcga ctcgcacgcc ctggagcagg ccatcaacga cgcgttctac
      721 gccgatccgg tcgtccgcga ggtcgtcgcg aagcggctgg cgctggaaac gcagctggtc
      781 aagaccaaga cgatgcgcaa ccctggtagc gaccccttca cgcgccatgt ccgcggaaaa
      841 atcaaggccg agaacgcacg tctcgatcag ctctggcaag cgatggagcc gaagctccgc
      901 cggcagcttg ccagcggcgg cggcgctgcc gacggcgacc acatcgtcgc ggcgaccgag
      961 cggcagttgg cctccctgac cgcgcaggag caggcgctgg aagaccggct cgaccagatg
     1021 acgctcaaga acgaccgggc gggcaacgac gcactcaaga tggatttcgc ccggatcgac
     1081 ctccagcggg ccgaagcggt gctcagccag gtgcaaacgc acctgcggca gctcgacttc
     1141 gagtcccgca acgccctggc gcgcatccag ctcgacttcg cggcccggcc gatgttacgg
     1201 cccagctcga accaccgtct gcacgcgatg ctcgccgcgc cgttggcgat gtggttcgcg
     1261 gtgctgggtc tgctcgggct catcgaattg aagtccgggc gcgtggcgga ccccgaggag
     1321 ctatcgagcc gctcgcggct ccaggtgctc ggcgtggtgc cacccttgcc gcgcatccgc
     1381 ccgtcggcgg gccagttggc gctcggcagc gacggccgcg cccagcgcca gctcgaagag
     1441 ttcgtgcaga gcctcgacca cctgcgcgtc gcgctctgcg cccggcccga tccgtggggc
     1501 cgcaaccgcc actgcgtctt gatcacaagc gcctgcggca gcgagggcaa aaccaccctc
     1561 gccgcgcagc tggccgagcg ctgcgtgaac gcgggcctga tgacgctctt gatcgacgcc
     1621 gacctgcgca acccgaccct gagccggatg ctcgacgccg agcaggaggc cgggctgatc
     1681 aacgtcctgc gcggcgagcg cgcgccggag gacgcgatca tcgtggtcga ggacgccggc
     1741 ggctttcact tcctgcccgc cggcacgccg cgagtggacc cgagccggct gctccagggc
     1801 gaccgcctgg gcaacctgct ggcgcaggcg cgcgagagct tcgagatgat catcgtcgac
     1861 gccccgcccg tgctgcccgt gcccgacgcc ctgaccatcg gccgctggac cgacggcgcc
     1921 gtgctggccg tacgctacga caacagccgc ttcccgctcg tcgatcgggc caatcgccgc
     1981 ctggcccacg tcggcgtccc ggtgatcggc gccgtcgtca acggcgtccg ctcgcccgac
     2041 ggcgcctacg ggggctacta cgcctatggc ggcagctcgt cggccgaacc ggcggtgtag
     2101 gacgagtgag cagcggggcg tgaggcgtga ggcgtgaaaa gccgaagact ctccaacttc
     2161 tcactcctca ccagcagcca agggacccag gagccaccga gcacgatgac gaccctgccg
     2221 atcaactcgc ccaccgcgcg cccgtcgtgg gccggctcgg ccctggtggt cgtggtggtc
     2281 accgcggcgc tggtcgcgtc gttcgcgcct tgcttcgtga acctcgtggg cctctgggac
     2341 gccgatccga acaacagcta cggctacctc gtcatcccga tggccgcgta catcctctgg
     2401 gaccgccgcc ggctgctcgt tccgcagtcg ctcgcgccct gctggtgggg cttcgcgccg
     2461 ctggcggccc tgctggcgct gcgggtcttg ctctacgagc gcaacgagca atacatggag
     2521 gcggccttga tcccgtgggt cgtcggctgc ctggtgctga tcctgggcgg ctggcacctg
     2581 ctgcgcgtcg cgctgccggc cgtc
//
LOCUS       DASUBI010000454         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1166011, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000454 DASUBI010000000
VERSION     DASUBI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166011"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFU72_08700"
     CDS             <1..547
                     /locus_tag="VFU72_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU5423604.1"
                     /translation="AAPDWTRTAPVANEVDARPLQEAGEEPFSAIMAGVRQVPSGGVL
                     RLYSTFEPTPLYDVLSRRGFDHFARQLAAGDWEILFLHRDEVAEPATRPADSAPAASD
                     EDWSGATASITIDVSELVPPEPLIRIMEALEELPPGATLLVHHVRRPIHLYARLDEEG
                     YRHQTREPAPGKVELLIEKPA"
     gene            544..1902
                     /locus_tag="VFU72_08705"
     CDS             544..1902
                     /locus_tag="VFU72_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423605.1"
                     /translation="MTVAPPRPTGISSAHAPDLDLPARFMALGMAALALVALVFPFAL
                     PLLAQGYAAPHVLAFVHLNTLGVVAAVIMGASYQLVPVVLQTPLASVALGRVTFWLHL
                     SGLALFLPGLWFTWTPGVGVGATLLFSGLLLYLGVIALTIARAPRRDVVAWHIAISLV
                     GLAGGVALGLLLALNQGAGFLGGLTLRLMAGHATLMLGSWVAVLLGGVSYRLAGMFTL
                     AEDRLSQPVAWSELALTALGAWGVALALVLSAPRPLLLLAGLVLLAGQVLFAGQLAHL
                     YRVRRRRGPDVHIPFALVAGGAGVASAALLVYGLAAGMGAGARLWPLVVWLALAGLAE
                     TAIQGFFYKIATFLVWLRRYAPQAGKRRVPKLEELYGQRLARAGWACWTLGLPLAAAG
                     LLSGSLLLCRLAGLAAGLGLAAFLVNVARIAAHWRAPTSVGTQRALRSPGTGAPEKGP
                     VS"
     gene            1899..2153
                     /locus_tag="VFU72_08710"
     CDS             1899..2153
                     /locus_tag="VFU72_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU5423606.1"
                     /translation="MSSNTTETYTLDVREIAPKVRHPLIFSSVDKMKLGETLRLVNDH
                     DPKPLYYQLMAERPGQVGWDYLESGPDVWQVRITRLATGE"
BASE COUNT          257 a          778 c          773 g          368 t
ORIGIN      
        1 ggcggcgccg gattggacac ggacggcgcc ggtcgccaac gaggtcgatg cccgcccgct
       61 gcaggaggcc ggcgaggagc ccttcagcgc catcatggcc ggggtgcgcc aggtgccgtc
      121 cggcggagtg ctgcgtctct actcgacctt cgagccgacg ccgctctatg acgtgctcag
      181 ccggcgcggc ttcgaccatt ttgctcgcca gctcgccgcc ggcgactggg agatcctctt
      241 cctgcaccgc gacgaggtgg ccgagccggc aacgcgcccg gccgactccg cgccggccgc
      301 gagcgatgag gactggtcag gcgcgaccgc cagtatcacc atcgacgtca gcgaattggt
      361 gccacccgag ccactgatcc ggatcatgga ggcgctggag gaactgccgc ccggcgcgac
      421 cctgctggta caccacgtgc gccggccgat ccacctctac gcccggctcg acgaggaagg
      481 ctaccggcac cagacgcgcg agccggcgcc cggcaaggtc gaactgttga tcgagaagcc
      541 cgcatgaccg ttgccccgcc ccggcccacc ggcatctctt cggcccacgc gccggacctc
      601 gatctccccg cgcgcttcat ggcgctgggc atggccgcgc tggcgctggt ggcgctggtc
      661 ttccccttcg ccctgccgct gctggcgcaa gggtacgccg cgccgcacgt gctcgccttc
      721 gtccatctca acaccctggg ggtcgtcgcc gccgtgatca tgggcgccag ttaccaactg
      781 gtgccggtgg tcttgcagac gccgctcgcc tcggtcgcgc tcggccgcgt caccttctgg
      841 ctccatctca gcggcctggc gctcttcctg cccggcctct ggttcacctg gacgcccggt
      901 gtcggggtcg gcgcgacgct gctgttcagc gggctgcttc tctatctcgg cgtgatcgcg
      961 ctcacgatcg cgcgggcgcc gcggcgcgat gtggtcgcct ggcatatcgc catctcgctg
     1021 gtggggctgg cgggcggggt ggcccttggg ctgctgctgg ccctgaacca gggtgccggc
     1081 tttctgggcg gtctcacgct gcgcctgatg gcgggccacg ccacgctgat gctgggcagt
     1141 tgggtggcgg tcctgcttgg cggcgtctcc tatcgcctgg ccggcatgtt cacgctggcc
     1201 gaggaccggc tctcgcagcc ggtcgcctgg tccgagctgg cgctgaccgc gctgggcgcc
     1261 tggggcgtgg cgctggcgct ggtcctgagc gcgccgcgcc ccctgctgct gctggccggc
     1321 ctcgtcctgc tcgcggggca ggtgctgttc gccggccaac tggcgcacct ctaccgcgtg
     1381 cgccggcgcc gcggccccga cgtccacatc cctttcgccc tggtcgcggg cggcgcgggg
     1441 gtggcatcgg ccgcgctgct ggtctatggc ctggcggccg gcatgggcgc cggcgcgcgt
     1501 ctctggccgc tggtcgtctg gctggcactg gccgggctgg ccgaaacggc catccaggga
     1561 ttcttctaca agatcgcgac cttcctcgtc tggctccgac gctacgcgcc tcaggcaggt
     1621 aagcggcggg tcccgaagct cgaggagctc tacggtcaac ggctggcccg cgcgggctgg
     1681 gcctgctgga cgctcggcct gccgctcgcg gcggcgggcc tgctgagcgg cagcctgctg
     1741 ctctgtcgtc tggccgggtt ggccgccggt ttgggactgg cagccttcct ggtcaatgtc
     1801 gcccgaatcg cggcgcactg gcgagcgccg acgagtgttg gtactcagcg tgcgctccgt
     1861 tcaccaggga cgggcgcgcc ggagaaagga cccgtgtcgt gagcagcaac accacggaga
     1921 cctatacgct ggacgtgcgc gagatcgcgc cgaaggtgcg ccacccgctg atcttcagta
     1981 gcgtcgataa gatgaagctc ggggagaccc tgcgcctggt caacgaccac gatcccaagc
     2041 cgctctacta tcaactgatg gcggagcgtc cggggcaggt cggctgggat tacctggaga
     2101 gcggccccga tgtttggcag gtgcggatca ccaggctggc aaccggcgag tagccggtcg
     2161 cggccggctt cgcacc
//
LOCUS       DASUBI010000455         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_930478, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000455 DASUBI010000000
VERSION     DASUBI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930478"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFU72_08715"
     CDS             <1..911
                     /locus_tag="VFU72_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU5423607.1"
                     /translation="TGPSLPLEAFRERMTTLGRSAVVVGDQQAIKVHIHSDHPGQILE
                     AALEYGELQNVRIDNMSAQTRALLADRQHQTQRAGGAAKPAGPAVVAVASGEGLAAAL
                     RGMGASAIVPGGPTVNPSTEEILRIVERLPNREIILLPNDKNVIPTARQVAALTDKQV
                     RVVPSETIPQAISALSAFNADAALDKNAVEMTAALPLVRSLALTRATRAVELDGLAVR
                     QDEFIGLLDGALHAAGPEPTAVIIRLLELAEASAAELATLFVGEPADDALVTAVTTAI
                     EARFPALTVEVAPGGQPHYHLIVALE"
     gene            922..1782
                     /locus_tag="VFU72_08720"
     CDS             922..1782
                     /locus_tag="VFU72_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5423608.1"
                     /translation="MRVAVVTDSTADIPGPLLARFGIHVVPLTLRLGTRTYDDGVTIT
                     PARFMDEVRQTDSLPATSQPSVARFQQLYLELGRDHDAIISIHLSSRLSGTYQNALLA
                     RDRINGRLPIAVLDSRSASMALGFPVLRAATLARAGHSLDEIVRAAEQAIGQSQLVFL
                     VDTLEYLRRGGRIGRAAEIVGTALQLKPILRIENGVVAPYARTRTRPRALASLVRLVY
                     EFPQVGNLAIMAAPGARDVRRLQNALAAICPPERTILTDLSPVLASHVGPGGLGVAVN
                     EGEPIALEGP"
     gene            1779..2636
                     /locus_tag="VFU72_08725"
     CDS             1779..2636
                     /locus_tag="VFU72_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5423609.1"
                     /translation="MSGARPVHIVTDSTADLTEELLAGQPITVVPLTVEIGGEVFRDG
                     IDLTREEFLERLRLGELPRTSQPPVGRFQQLYQELIGSGHDIVSVHISSGLSGTYNAA
                     HTAARAVAPERIRVVDSRTVSMGLGWLALEAAGMAREGQDCAAIADYLEVRKADARIV
                     ALLDTLEFLWRGGRIGRTSAFLGSALQIKPLVAVAEGEVQPLERVRTLKRALDRLVEL
                     AGEMQPFDHLAVLHLGTPEAAATLADRLALVQPDLELVRAQLGTVIGVYSGPGIMGFA
                     GLIARERSG"
BASE COUNT          384 a         1012 c          919 g          377 t
ORIGIN      
        1 tcaccggacc ctcgttgccg ctggaggcgt ttcgcgagcg catgacgacg ctcggccgct
       61 cggcggtggt ggtcggcgac cagcaggcga tcaaggtcca tatccacagc gaccatcccg
      121 gccagatcct cgaagcggcg ctggagtacg gcgagctgca gaacgtgcgc atcgacaata
      181 tgtcggcgca gacgcgcgcc ctgctggccg accggcagca tcagacgcag cgagccggcg
      241 gcgccgcgaa acctgccggg ccggcggtgg tggcggtcgc cagcggcgag gggctggcgg
      301 cggcgctgcg cggcatggga gccagcgcca tcgtgccggg cggtcccacc gtcaacccga
      361 gcaccgagga gatcctgcgc atcgtcgagc gcctgcccaa tcgcgagatc atcctcctgc
      421 ccaacgacaa gaacgtcatc cccaccgcgc gccaggtggc cgcgctgacc gacaagcagg
      481 tgcgcgtagt gccctcggag acgatcccgc aagcgatcag cgcgctctcg gccttcaacg
      541 ccgatgccgc actggacaag aacgccgtcg agatgaccgc cgccctgccg ctggtgcgct
      601 cactcgccct gacccgcgcc acccgcgccg tcgagctgga cggcctggcg gtgagacaag
      661 acgaattcat tggcctgctc gacggcgcct tgcatgccgc cggtcccgag ccgacagcgg
      721 tcatcatccg gctgctggag ctggccgagg ccagcgccgc cgagttggcc accctcttcg
      781 tgggcgaacc cgccgatgac gcgctggtca ccgccgttac caccgcgatc gaggcgcgct
      841 tcccggcgct gaccgtcgaa gtagcacccg gcggacagcc gcactaccac ctgatcgtgg
      901 cgctggagtg agcctgtgac tatgcgcgtc gccgtcgtca ccgacagcac cgccgacatc
      961 cccgggccgc tgctggcccg cttcggcatc cacgtagtgc cgctgacgct gcggctcggc
     1021 acgcgcacct acgacgacgg cgtcaccatc accccggccc gcttcatgga cgaggtacgt
     1081 cagaccgaca gcctgcccgc cacctcccag ccctcggtgg cccgcttcca gcagctctac
     1141 ctggagctgg gccgcgacca cgacgcgatc atctcgatcc acctctccag ccgcttgagc
     1201 ggcacctacc agaacgcgct gctggcgcgc gaccggatca acgggcgcct gccgatcgcg
     1261 gtactcgact cgcgctcggc ctcgatggcg ctcggctttc ccgtgctgcg cgccgccacg
     1321 ctggcccgcg ccggtcactc gctcgacgag atcgtgcgcg ccgccgagca ggcgatcggc
     1381 cagagccagc tcgtcttcct ggtcgacacg ctggagtacc tgcggcgcgg cgggcgtatc
     1441 gggcgcgcgg ccgagatcgt cggcacggcc ctgcaactca agccgatcct gcgcatcgag
     1501 aacggcgtgg tcgcgcccta cgcgcgcacc cgcacccgtc cgcgcgcatt ggccagcctg
     1561 gtccggctgg tctacgagtt cccccaggtc ggcaacctgg cgatcatggc cgcgcccggc
     1621 gcgcgtgacg tgcgccggct gcagaacgcc ctcgccgcga tctgcccacc cgagcgcacc
     1681 atcctgaccg acctctcgcc ggtgctggcc agccacgtcg ggccgggcgg gctcggggta
     1741 gcggtcaacg agggcgagcc gatcgcgctg gagggtccgt gagcggcgcg cgcccggtgc
     1801 acatcgtcac cgacagcacg gccgatctca cggaggagct gctcgccggc cagccgatca
     1861 cggtcgtgcc gctgacggtc gagatcggcg gcgaggtctt tcgcgacggc atcgacctga
     1921 cgcgcgagga gttccttgag cgcctgcggc tgggtgagct gccgcgcacc tcgcagcctc
     1981 cggtcggccg cttccagcag ctctaccagg agctgatcgg cagcggccac gacatcgtgt
     2041 cggtgcacat ctcctccggt ctctccggca cctacaacgc cgcccacaca gccgcgcggg
     2101 cggtcgcccc ggagcgcatc cgggtggtcg actcgcgtac cgtctcgatg ggcctgggct
     2161 ggctggccct ggaggcggcc ggcatggccc gcgagggtca ggactgcgcg gcgatcgccg
     2221 actacctgga ggtgcgcaag gcggacgcgc gcatcgtggc gctgctcgac acgctggagt
     2281 tcctgtggcg cggcgggcgc atcggccgca cctcggcctt cctgggctcg gcgctgcaga
     2341 tcaagccact ggtggcagtc gccgagggcg aggtgcagcc actggagcgg gtgcgcaccc
     2401 tcaagcgggc gctcgatcgc ctggtcgagc tggccggcga gatgcagccc ttcgaccacc
     2461 tggccgtgct ccacctgggc acgcccgagg ccgccgccac cctggccgac cggctggccc
     2521 tcgtgcagcc cgatctcgag ttggtgcgcg cccagctcgg caccgtcatc ggggtctaca
     2581 gcgggccggg gatcatgggt ttcgccgggc tgatcgcccg cgaacgatcg ggttgacagc
     2641 gcccgcgccg ccgcgttcac ttgcgctatg acgcgcgagc gggaccagga cg
//
LOCUS       DASUBI010000456         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1356298, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000456 DASUBI010000000
VERSION     DASUBI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356298"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1284
                     /locus_tag="VFU72_08730"
     CDS             319..1284
                     /locus_tag="VFU72_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEU5423610.1"
                     /translation="MATARIGVKTGWRATVWGELLAEFLGTFVLIALGDGVVAMAVAA
                     LNSSGRAANPTTIFLASGDWLLIVWGWAFAVIFGVYVAGGVSGAHINPAVTLALAVRR
                     GFPWAKVIPYWIAQILGAFVGAALVFLDYHDAISAFEKAANIVRDGAGGNTTFSIFAT
                     FPAPYYNGSLIGPLIDQIIGTALLVLLIFAVVDTLNQAPGMNLGAFIIGLIVAAIGIS
                     FGANAGYAINPARDFGPRVFAFIAGWGKNAFPGVSSYWWVPIVGPLIGGVIGAFIYDL
                     FIHDVLVARGAAPTPGEVEHGRTVEETGIPGDVDQRGRTVREDRS"
     gene            1343..2344
                     /gene="dhaK"
                     /locus_tag="VFU72_08735"
     CDS             1343..2344
                     /gene="dhaK"
                     /locus_tag="VFU72_08735"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU5423611.1"
                     /translation="MKKLINQPEMVVREALEGMALAHPDLLRVDPELQLIVRKDAPVA
                     GKAGVVSGGGSGHEPMHGGFVGMGMLDAACPGAVFTSPVPDQMLAAAKAVDGGAGVLY
                     IVKNYTGDVMNFELAAELAQAEGIETRSVVINDDVAVENSTFTAGRRGVGGTVLAEKI
                     TGALAQRKASLDEVTAVCQKVNDNVRSMGMALSPSTVPAAGKPGFELGENEMEIGIGI
                     HGEPGRRRVPLASADEIAEMLVTPIVEDLPFRRGDQVLAFVNGMGGTPLIELYIMFRA
                     VYGLLEQRGITVARSLVGSYITSLEMAGSSVTLLRLDDQLTQLWDAPVHTAALRWGV"
     gene            2474..3124
                     /gene="dhaL"
                     /locus_tag="VFU72_08740"
     CDS             2474..3124
                     /gene="dhaL"
                     /locus_tag="VFU72_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU5423612.1"
                     /translation="MAETESIGLRILRRVQAVIHQHADELTALDRPIGDSDHGINVDR
                     GFTAALAKVEAQPDQSLAAVLKTIATTLISTVGGAAGPLYGTAFLRAATAYAQPADSE
                     QQRVEAAFEAALGGVQARGKATTGEKTMVDALAPAVTALRAAFAEGAGIADALEQAAA
                     AAHAGMEATIPMLATKGRASYLGERSIGHQDPGATSSYLIVQTIADTVRDEKQDGG"
BASE COUNT          499 a         1112 c         1119 g          471 t
ORIGIN      
        1 agggacaatg tgcccgacgg tcagcttgcg cacaaggtgc gcgcggcaca agtcttggca
       61 tgcttctggc gggcagcatg gcggtacgtc cagggagacg aactgagatc gactccgatc
      121 agtcaggcgc gtgaatggca gcaggcgccc ctgcgtgggc tcgggaaggg aggtaatcgc
      181 catccgtcac gctcggtcca tccacgcatg atctatccac cacgcacgat cgctcgcggg
      241 cgcgctggcc tggccgcgaa cccgcgacgg tctcggacgg cgcgccgggc ggcgcggccg
      301 tcggaaggag cgggttgtat ggcgacagca cggattgggg tcaaaaccgg ctggcgagcg
      361 acggtctggg gggagctgct cgccgaattc ctcggcacct tcgttctgat cgcgctcggc
      421 gacggcgtag tcgccatggc ggtggccgcc ctgaactcct cgggccgcgc ggccaacccg
      481 accaccatct tcctggcctc cggcgactgg ctcttgatcg tctggggctg ggccttcgcg
      541 gtcatcttcg gcgtctacgt cgccggcggc gtcagcggcg cgcacatcaa cccggcggtg
      601 acgctggcgt tggccgtgcg gcgcggcttc ccctgggcga aagtcatccc ctattggatc
      661 gcgcagatcc tcggcgcctt cgtcggcgcc gcgttggtct tcctcgacta tcatgacgcg
      721 atcagcgcct tcgagaaggc cgccaacatc gtgcgcgacg gcgcgggcgg caataccacc
      781 ttcagcatct tcgccacctt ccccgccccc tactacaacg gcagcctgat cggcccgctg
      841 atcgaccaga tcatcggcac cgctttgctg gtgctcctga tcttcgccgt ggtcgacacc
      901 ctcaaccagg cgcccggcat gaacctcggt gccttcatca tcggtctgat cgtggccgcc
      961 atcggcatct ccttcggcgc caacgccggc tacgcgatca acccggcgcg cgacttcggc
     1021 ccgcgcgtct tcgccttcat cgccggctgg gggaagaacg ccttccccgg tgtcagcagc
     1081 tactggtggg tgccgatcgt cggcccgctg atcggcggcg tgatcggcgc cttcatctat
     1141 gatctcttta tccacgatgt actggtcgca cgcggcgccg cgcccacccc gggcgaggtg
     1201 gagcacggcc gcaccgtcga ggagaccggc attccgggcg acgtcgatca gcgcggccgg
     1261 accgtgcgcg aggatcggag ctagtcaccg ggccctgcgg ccggcgcgcg gcttccccga
     1321 gggggtagca aggaggacgc tcgtgaagaa actgatcaac caacccgaga tggtggtgcg
     1381 cgaggcgctg gaagggatgg cgctggcgca ccccgacctc ttgcgcgtcg atcccgagct
     1441 gcaattgatc gtgcgcaagg acgcgccggt cgcgggcaag gccggcgttg tctccggcgg
     1501 cggcagcggc cacgagccga tgcacggcgg cttcgtcggc atgggcatgc tcgatgccgc
     1561 ctgccccggc gccgtcttca cctcgccggt gccggatcag atgctggcgg cggccaaggc
     1621 ggtcgacggc ggcgccgggg tgctctacat cgtcaagaac tacaccggcg acgtgatgaa
     1681 cttcgagctg gcggccgagc tggcccaggc cgaggggatc gagacccgca gcgtcgtgat
     1741 caacgacgac gtcgccgtcg agaacagcac cttcaccgcc gggcgccgtg gggtcggcgg
     1801 cacggtgctg gccgagaaga tcaccggggc gctggcccag cggaaggcca gtctggacga
     1861 ggtcacggcg gtctgccaga aggtgaacga caacgtgcgc agcatgggca tggcgctgtc
     1921 gcccagcacc gtgccggcgg cgggcaagcc gggcttcgag ctgggcgaga acgagatgga
     1981 gatcggcatc ggcatccacg gcgagccggg gcgacggcgt gtgccgctgg cctcggccga
     2041 cgagatcgcc gagatgctgg tcaccccgat cgtggaggac cttcccttcc gccggggcga
     2101 ccaggtgctg gccttcgtca acggcatggg cggcacgccg ctgatcgagc tctacatcat
     2161 gttccgcgcg gtctacggtt tgctggagca gcgggggatc acggtagcgc gctcgctggt
     2221 cggcagctac atcacctcgc tggagatggc cggctcgtcg gtcacgctgc tgcggctcga
     2281 cgaccagctc acccagctct gggacgcgcc ggtccacacc gccgcgctcc gctggggcgt
     2341 gtgaaccctc acccctaccc ctctcccgtg cgcgggagag gggagtacga agcgtcaccg
     2401 caacgtactc ctccttctcc cagcattggg agaagggggc gggggagatg agggcccgta
     2461 gcaaaggaac accatggctg agacggagag catcgggctg cggattctac ggcgcgtcca
     2521 ggcggtcatc caccagcacg ccgacgagct gacggcgctc gaccggccga tcggcgacag
     2581 cgaccacggc atcaacgtgg accgcggctt caccgccgcg ctggccaagg tcgaagctca
     2641 gccggaccag tcgctggccg ccgtgctcaa gacgatcgcc accaccctga tctcgaccgt
     2701 cggcggcgcc gccgggccgc tctacggcac cgccttcctg cgcgccgcca ccgcctacgc
     2761 ccagccggcg gacagcgagc agcagcgggt ggaggcggcc ttcgaggccg ccctgggcgg
     2821 cgtgcaggcg cgcggcaagg cgacgaccgg cgagaagacg atggtcgacg cgctggctcc
     2881 ggcggtgacg gcgctgcgcg cggccttcgc cgaaggcgcc ggcatcgcgg acgcgctgga
     2941 gcaggccgcc gccgccgccc acgccggcat ggaggccacc atcccgatgc tggcgaccaa
     3001 ggggcgcgcc agctacctgg gcgagcgcag catcggccac caggaccccg gcgccacctc
     3061 cagctacctg atcgtccaga ccatcgccga taccgtccgc gacgagaagc aggacggtgg
     3121 ctagctaagc gggcgaccgg gcggtcgtcc cgcggacact tcgaagcttg aaaggagcca
     3181 gaccgtggcg gatgctatcc t
//
LOCUS       DASUBI010000457         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1337045, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000457 DASUBI010000000
VERSION     DASUBI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337045"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="rsmA"
                     /locus_tag="VFU72_08745"
     CDS             complement(<1..794)
                     /gene="rsmA"
                     /locus_tag="VFU72_08745"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5423613.1"
                     /translation="MGERRSDWRALLDELGVRPSRSKGQNFLHDPAIVRRIAEASEIA
                     PGELVLEIGPGLGVLTRELAARAGRVIAVELDRRLAAHLAGAGIAGVDVVEADILATD
                     VAVLTGGQRYVVVANLPYNIAAAVIEHLLSSPARPQRLLVMVQREVAERIVARPPKMS
                     ILAVAVQFYGRPRVVLRVGPGAFIPAPKVESAVLRIDVAPEPPLPERAQAGFFRLVRA
                     GFGQRRKQLANALAAGLRQPKATVEAALHEAGIAPERRAETLTVDD"
     gene            complement(838..1878)
                     /gene="selD"
                     /locus_tag="VFU72_08750"
     CDS             complement(838..1878)
                     /gene="selD"
                     /locus_tag="VFU72_08750"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1825..1827),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU5423614.1"
                     /translation="MQSRDEIRLTTLASCAGUASKLGPGALSAILSGLSRRTHPNVLV
                     GLQTSDDAAVYRLSDELALIQTVDFFTPIVDDPYTYGAIAAANSMSDVYAMGGEVLVA
                     LNIAAFPEDLPASVIERIFEGGADKVAEVGAVIAGGHTVTDDEPKYGLSVTGTIHPER
                     ILTKAGARPGDILYLTKPLGTGVIATALKNGAAEAGDVAEAVEWMLLVNRAAARLSVS
                     VGIDACTDITGFGLVGHASEIAAKSGVQVIIEAGSVPLMAGAERYAAEGFLPGGGGRN
                     RDYYRRVTTLAPEVSPVLADLLFDPQTSGGLLLAVPERRAGQLEAAFAASGDPLWRIG
                     HVTAGAGVAVVL"
     gene            complement(1969..2337)
                     /locus_tag="VFU72_08755"
     CDS             complement(1969..2337)
                     /locus_tag="VFU72_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423615.1"
                     /translation="MEQSPGDLERRFATEGDQELDPTDALYIVKPETPVVDRDGDEIG
                     KVVRVYRPVTVDEMGSQATVAYLRVGHRGFLGLAADLYVPSDVVESVDEHEVRLSVDR
                     DVIPDMVWDRPYNIAQDEDE"
     gene            2536..>3347
                     /locus_tag="VFU72_08760"
     CDS             2536..>3347
                     /locus_tag="VFU72_08760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5423616.1"
                     /translation="MHRARRRSLTLIFAALLLAALALNALPARSSAASEPPAARTITR
                     VSTSDKVMALTFDAGSDRGYASQILDTLSAKGVKATFSPTGVWAQQNPDLVKRMVADG
                     HQLMNHSWDHPDFTQISSAQRVDQLQRSQQFIQQLTGYDVRPFFRPPFGAYNDQVLFD
                     LEANGYSYNVMWTVDTLGWQGASVAAITSRTMAAAAPGAIVLMHVGADSQDAAALPGI
                     IDQLRALGYRFDTVAHLLGVPPPAPGAGRYFPQTGHWLSHGFLRYWEQFGGLP"
BASE COUNT          497 a         1212 c         1125 g          513 t
ORIGIN      
        1 caatcgtcaa cggtgagcgt ctcggcgcgg cgctcgggcg cgatcccggc ctcatgcagc
       61 gccgcctcga ccgtcgcctt cggctgccgc agcccggccg ccagcgcgtt cgccaactgc
      121 ttgcgtcgct ggccgaagcc ggcccgcacc aggcggaaga agccggcttg cgccctctcc
      181 ggcagcggcg gctccggcgc gacgtcgatg cgcagcaccg ccgattcgac cttcggcgcc
      241 gggatgaagg cgccggggcc gacgcgcagc acgacgcgcg gccggccgta aaactgtacc
      301 gccaccgcca ggatactcat cttcggcggc cgggccacga tgcgctcggc cacctcgcgc
      361 tgcaccatca ccagtagccg ctgcggccgc gccgggctgg agaggaggtg ctcgatgacc
      421 gcggcggcga tgttgtaggg gaggttggcg acgaccacat agcgctggcc accggtcagt
      481 acggctacat cggtggcgag gatgtcggcc tcgaccacgt cgaccccggc gataccggcg
      541 ccagccaggt gcgcggccag ccgccggtcc agctcgaccg cgatgacccg gccggcgcgc
      601 gccgccagct cgcgtgtcag cacgccgagc cctgggccga tctccagcac cagctcgccg
      661 ggtgcgatct cggacgcctc tgcgatgcgc cgcacgatcg ccggatcgtg caggaagttc
      721 tgtcctttgg acctactcgg ccggacgccc agctcgtcga gcagggcgcg ccagtccgag
      781 cgccgctcgc ccacgcgcgc tccttcaggc aaagaacccc gttgcgctgg ccagcgttca
      841 gagaacgacc gcgacgcccg ctcctgccgt cacgtgcccg atgcgccaga gcgggtcgcc
      901 ggacgcggcg aaggccgctt cgagttgacc cgcgcgccgc tcaggcacgg ccagcagcag
      961 cccgcccgac gtctgcggat cgaagaggag atcggcaagc accggagaga cctccggcgc
     1021 cagagtcgtc acgcgccggt agtagtcgcg gttgcgcccg ccgccgcccg gcaggaagcc
     1081 ctcggcggcg tagcgctcgg cgcctgccat cagcggcacg ctgccagcct cgatgatgac
     1141 ctgcacgccg ctcttggcgg cgatctcgct ggcgtggccg accaggccga agccggtgat
     1201 gtcggtgcag gcgtcgatcc cgaccgagac actcagccgc gctgccgccc ggttgaccag
     1261 cagcatccac tcgaccgcct ccgcgacgtc gccggcttcc gccgcgccgt tcttgagcgc
     1321 ggtggcgatc accccggttc ccagcggctt ggtcaggtag aggatatcgc cggggcgcgc
     1381 gccggccttg gtcaggatgc gctcgggatg gatggtgccg gtgaccgaga gaccgtactt
     1441 cggctcgtcg tcggtcacgg tgtggccgcc ggcgatcacc gcgccgacct cggccacctt
     1501 gtccgcgccc ccttcgaaga tgcgctcgat cacgctggcc ggcaggtctt cggggaaggc
     1561 ggcgatgttg agcgccacaa ggacctcgcc gcccatggcg tagacgtcgc tcatcgagtt
     1621 ggcggccgcg atcgccccgt aggtgtaggg gtcgtcgacg atcggggtga agaaatcgac
     1681 ggtctggatc agcgccagct cgtcgctgag ccggtagacg gcggcgtcgt cgctggtctg
     1741 cagcccgacc agcacgttgg ggtgcgtgcg gcggctgagc ccgctgagaa tggcggagag
     1801 ggcgcccggc cccagtttgg atgctcagcc ggcgcaactg gccagcgtcg tcagccgaat
     1861 ctcatcgcgc gactgcatcc gtcgcatcct ctccgctggg ctgctccgca acccgccgta
     1921 taccctctgg ttccccaacg cggcggggcg cccgcctgtt cccgacggtt attcgtcctc
     1981 gtcctgggcg atgttgtagg gccgatccca gaccatgtcg gggatcacgt cgcgatcgac
     2041 gctgagccgc acttcatgct cgtcgaccga ctccacgacg tcggagggca cgtagaggtc
     2101 agcggcgagg cccaggaagc cgcgatgccc cacccggagg taggccacgg tagcctgcga
     2161 gcccatctcg tcgaccgtca ccggccggta gacgcgcacc accttgccga tctcgtcgcc
     2221 gtcgcgatcg accaccggtg tctccggctt gacgatgtag agggcatcgg tcgggtccag
     2281 ctcttgatcg ccctcggtgg cgaagcggcg ctccagatca ccaggactct gctccatggc
     2341 gcggcactcc cttctcccgc cggcggcggg tcacggccct cccggcacga agaatgccac
     2401 gcgccggcac tggttgaccc gtccggaccg aattgagacg agattgacat accgcgctga
     2461 tcggcgcgac atgggtgata ctgccggcgt gcgagcgtta agccaggacc gatcgagtct
     2521 gggaaggatt cacacatgca cagggcacgg cgccgttcgc tgacgctcat cttcgcggcg
     2581 ttgctgctgg ccgcactcgc gctgaacgcc ctgccggcgc gctcgagcgc ggcgtccgaa
     2641 ccgcccgccg cgcgcaccat cacgcgcgtc tcgaccagcg acaaggttat ggccttgacc
     2701 ttcgacgccg gctccgatcg cggctacgcc tcgcagatcc tcgacacgct ctccgccaag
     2761 ggtgtcaaag cgaccttcag cccgaccggt gtttgggccc agcaaaaccc cgacctggtc
     2821 aagcgcatgg tcgccgatgg ccaccagttg atgaatcaca gctgggatca tccggacttc
     2881 acccagatca gctcggcgca gcgcgtcgac cagttgcagc gctcgcagca gttcattcag
     2941 caactgaccg gctacgacgt gcgcccgttc ttccggccgc cgttcggcgc ctacaacgac
     3001 caggtactgt tcgatctgga ggcgaacggc tacagctaca acgtcatgtg gacggtggat
     3061 acgctcggct ggcagggtgc ctcggtggcc gcgatcacca gccggaccat ggccgccgcc
     3121 gcgcccggtg ccatcgtgct gatgcacgtg ggcgcggatt cgcaggacgc ggccgcgctg
     3181 ccgggtatca tcgatcaact gcgcgccctg ggttatcgct tcgataccgt ggcgcacctg
     3241 ctcggcgtgc ccccgccggc gccgggggcg gggcgctact tcccgcagac ggggcactgg
     3301 ctcagccacg gctttctgcg ctactgggag cagttcggcg gcctgcc
//
LOCUS       DASUBI010000458         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1056046, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000458 DASUBI010000000
VERSION     DASUBI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056046"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFU72_08765"
     CDS             complement(<1..987)
                     /locus_tag="VFU72_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423617.1"
                     /translation="MVEGQGSPFGVRLRRLRESAGLSQEQLAERAGLSANAIGALERG
                     DRQRPYPNTVRALSDALELAPAEREELATLARGRAEPAARPVPDGLPPEAALPLPPTP
                     LIGREAETTAVAALLAGGEQRLLTLTGPGGVGKTRLAVAAAHAAAPAFPDGVAYVPLA
                     PLADPALLLPTIARAVGLRDRVELPPSELLAEHLRAQRLLLVLDNLEHLLDAAPEIAE
                     LLESCPRLSVLATSRAALRLRAEQEYPVEPLPLPELRHVPSADEVAAAPAAQLFVARA
                     RASAPGFSLSDDNAPSIAAICHQLDGLPLALELAAAWVKLLPPSALLARLDRT"
     gene            1192..2790
                     /locus_tag="VFU72_08770"
     CDS             1192..2790
                     /locus_tag="VFU72_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423618.1"
                     /translation="MSAALFTESLAGILETTMARLLLERSETGEEVSLHPRLALAARA
                     GWLGLILVSLGLFLASLPLRYHQLHDFSFVPLARSDLLRAIDPETLHRALSGLGLSTT
                     VFAWFSLTLEVAFAGAFCAVAALIAARRDNTWWIKFVVLMMVVFGTSSLPTITALEGT
                     NALVELLARITHQLGWALVEIFLLLFPSGRFVPRWTGYAAGFFLVWMLAMAVFPDSLV
                     SAFTWPDQLGYLLWLVWYGGGMAAQLYRFRHHANRKERWQTKWTLFSVIVAVIGTLAV
                     NVPAIFAPAQSGGAAGELCELIRIPAGALFIMIMPVTVGIAILRYRLWEIDLLISRTL
                     VYLPLTAILGGLYTASISLFQRLFEATTGNSSDAAIVLTTLVVASAFTPVKNALQGAV
                     DGRFKPAGQDTDLRAYRRQLQAIVGVLDMQQAMGNLLELAAASLAARGGAIYLLAGGA
                     LDPVQTVGAWHGHEEAVVSVPLEWQGVRLGLVALGPKRNGQAYSEDDLAELRQAADSL
                     AHLAVLRGGHLEAAAAEPLLTQPA"
     gene            2833..>3166
                     /locus_tag="VFU72_08775"
     CDS             2833..>3166
                     /locus_tag="VFU72_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423619.1"
                     /translation="MRVESREGTADVLIDSQASHDRLALVEVVVRRGEELPRHRHHWE
                     DETLYVLSGEVAVWHEAVWRSLRPGEGIFLSRGGEHAVAALSPVARLLVTVTPAGFED
                     FYRALGEAG"
BASE COUNT          409 a         1104 c         1160 g          493 t
ORIGIN      
        1 ggtgcggtcg agccgcgcca ggagcgcgct cggcggcagg agcttgaccc aggcggccgc
       61 cagctccagc gccagcggca agccgtcgag ctggtgacag atggccgcga tcgagggcgc
      121 gttgtcgtcg ctcaggctga agccgggcgc gctggcgcgg gcgcgcgcca cgaagagctg
      181 tgccgccggg gcagcagcca cctcgtccgc gctgggaaca tgacgcagct ccggcagtgg
      241 aagcggctcg accggatact cctgctcggc gcgcaggcgc agcgcggccc ggctggtcgc
      301 cagtacgctg agccgggggc aagactccag cagctccgcg atctccggcg ccgcgtccag
      361 caggtgctcc aggttgtcga gcaccagcag cagccgctgc gcccgcaggt gctccgcgag
      421 cagctctgac gggggcagtt cgacgcggtc gcgtaggccg acggcacggg cgatcgtcgg
      481 aagcagcagc gccggatcgg ccagcggggc cagcggcacg taggccacac catcggggaa
      541 ggccggcgcg gcggcgtgcg ccgccgccac cgccaggcgc gtcttgccca cgccgccggg
      601 gccggtcagc gtcagcaacc gttgctcgcc gccggccaac agcgccgcga ccgcggtggt
      661 ctccgcctcc cgtccgatca atggcgtcgg cgggagcggc agggccgcct cgggcggcag
      721 tccgtccggc accgggcgcg ccgcgggctc ggcgcggcca cgcgccaggg tggcgagttc
      781 ctcgcgctcg gcgggcgcga gctccagtgc gtcggagagg gcgcggacgg tgttggggta
      841 ggggcgctgg cggtcgccgc gctccagcgc gccgatggcg ttggccgaga gcccggcgcg
      901 ctcggccaat tgctcctggc tcagcccggc ggactctcgc agtcggcgca gccgcacgcc
      961 gaacggactc ccctgaccct cgaccatgct cttcaccccg gctcgttcgg ccgcttgtcg
     1021 cgcgggcacc atcgtatcac gcggcgtcgc tacacagcag cgagtgtggg ggagtgtgaa
     1081 gttgctgttg gtggtgcctc gtctgggtgg ggcccagact gagacgcaga tcgagattgg
     1141 cccgcgccga gacggcggcc tgtcgcggag cgttcgaccg ggagtgtgac aatgtcagca
     1201 gcactgttta ccgaatcgct ggccggcatt ctggagacca ccatggcccg gctgctgctg
     1261 gagagatccg agacgggcga ggaagtgtcg ctgcacccgc gcctggcgct cgccgcgcgc
     1321 gccggctggc tcgggctgat cctggtgtcg ctcggcctct tcctggccag cctgccgctg
     1381 cgctaccatc agctgcacga cttcagcttc gtgccgctgg cccgctccga tctgttgcgg
     1441 gcgatcgacc cggagacgct gcaccgggcg ctgagcggcc tcggcctctc gaccacggtc
     1501 ttcgcctggt tcagcctgac gctggaggtc gccttcgccg gcgccttctg cgcggtggcg
     1561 gccctgatcg ccgcgcgccg cgacaacacc tggtggatta agttcgtggt gctgatgatg
     1621 gtcgtcttcg gcacctcgtc cttgccgacg atcaccgcgc tcgagggcac caacgcgctg
     1681 gtcgagctgc tggcccgcat cacgcaccag ctcggctggg cgctggtcga gatcttcctg
     1741 ctcctcttcc ccagcgggcg cttcgtgccg cgctggaccg gctacgcggc cggcttcttt
     1801 ctggtctgga tgctggcgat ggcggtcttc ccggactcgc tcgtcagcgc cttcacctgg
     1861 ccggaccagc tcgggtatct cctctggctg gtctggtacg ggggcgggat ggccgcgcag
     1921 ctctaccgct ttcgccacca cgccaatcgc aaggagcgct ggcagaccaa gtggaccctc
     1981 ttcagcgtga tcgtggcggt gatcggcacg ctggcggtca acgtaccggc gatcttcgcc
     2041 cctgcgcagt cgggcggcgc ggccggtgag ctctgcgagc tgattcgcat cccggccggc
     2101 gcgctcttca tcatgatcat gccggtcacg gtcggcatcg cgatcctgcg ctaccggctg
     2161 tgggagatcg acctgctgat cagccgcacg ctggtctacc tgccgctgac ggccatcctc
     2221 ggcggcctct acaccgcctc gatcagcctc ttccagcggc tcttcgaggc cacgaccggc
     2281 aatagctccg acgcggccat cgtgctgacc accctggtgg tcgcgtcggc cttcactccg
     2341 gtcaagaatg cgctgcaggg ggcggtcgac ggccgcttca agccggccgg gcaggacact
     2401 gacctgcgcg cctaccggcg ccagctccag gccatcgtcg gggtgctcga catgcagcag
     2461 gcgatgggca acttgctgga gctagcggcc gcctcgctgg cggcgcgcgg cggcgcgatc
     2521 tacctgctgg ccggtggtgc gcttgacccg gtgcagacgg ttggcgcctg gcatgggcac
     2581 gaggaggcgg tggtcagcgt gccgctggag tggcaaggcg tgcggctcgg tctggtcgcg
     2641 ctcggaccca agcgcaatgg gcaggcctac agcgaggacg acctggccga gctgcggcag
     2701 gccgccgaca gcctggccca cctggcggtg ctgcgcggcg gtcacctgga agcggccgcc
     2761 gccgaacccc tgctgacgca gccggcttga tggagtgcag cgggcgatca gtagcggaaa
     2821 ggagcgccca cgatgcgcgt tgagtcacgg gaaggcacgg cggacgtgct gatcgatagt
     2881 caggcgagcc acgatcgcct ggcgctggtc gaggtcgtgg tgcggcgcgg cgaggaattg
     2941 ccgcgtcacc gccaccattg ggaagacgag acgctctacg tgctctccgg cgaggtggcc
     3001 gtctggcatg aggccgtctg gcgcagcctg cgtcccggcg agggaatctt cctgtcgcgc
     3061 ggcggcgagc acgcggtggc ggccctcagc ccggtcgcgc gcctgctggt caccgtgaca
     3121 cccgccggtt tcgaggactt ctaccgtgcg ctcggggagg ccggcg
//
LOCUS       DASUBI010000459         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_658661, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000459 DASUBI010000000
VERSION     DASUBI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658661"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFU72_08780"
     CDS             complement(<1..497)
                     /locus_tag="VFU72_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5423620.1"
                     /translation="MDLERLQRFLAESGVASRRHAEELIQEGRVTVDGEVVRYLGTRV
                     DPARQRIEVDGKLVRPQRKRYLVLNKPAGYITTAQDERGRRTVLDLVQVPERVVPVGR
                     LDRQTSGLLLLTNDGELAYRVAHPKFELTKEYLALLDGHPPPEVLERLRRGVVVDGER
                     VSTEQ"
     gene            complement(527..1699)
                     /locus_tag="VFU72_08785"
     CDS             complement(527..1699)
                     /locus_tag="VFU72_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU5423621.1"
                     /translation="MAASTPAALALMGASPRLGVDHAPAAPSAVIATSTPTPAATPRP
                     VAPALQVQLSARAAFAVDLTHLVELYAANADTPLPPASTTKIVSALVALRFLPLETKV
                     TVQPQDLVDPAIYSHMGLETGDVVTARDLLVGMLLPSGDDAALALARATGERLPAADG
                     ADPMARFVAEMNRLAASLGMDHSHFLNPDGVDAPGHVSTARDLAIATAALFQHPLLEQ
                     LVGAQTETEQVGGPNARSITLVNTNELLGQPGVHGVKTGTTDAAGQCLITAVWRGGSR
                     IVTVVLGSGDRYADTDALLNALDGQYRWLRLGRDGDIAPLNQLLAEKHLAFGVVVPVI
                     LGRDQAADLHYVLQLAPRPGGGWTPQGSVLFSSGGEPILRLPVYSGDPARSGTPAP"
     gene            1992..3098
                     /gene="hisC"
                     /locus_tag="VFU72_08790"
     CDS             1992..3098
                     /gene="hisC"
                     /locus_tag="VFU72_08790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU5423622.1"
                     /translation="MASIPKALRRIIRPSVLEQDGYVPSDDLLAVARDLGRSVDEISK
                     LDTNENPYGTSIHVQEALASFDRYHRYPDADQRAARGRLAEYTGAAAERIIIGNGADE
                     LIDLLMLATIDPGDEVIVPVPTFGVYAQRAALFHGEARAVSRTADYELDVDAIAQAVN
                     ERTKLIFVASPNNPTGNLVTSQQLVRLLRTGALVVLDEAYYEFSGKTFLPLVGEWDNL
                     VVLRTFSKWAGLAGLRIGYGIFPPALASGIWKVKQPFNVSVAALAAIEAVLDDQEYLR
                     ERVSRIRVERGRLFRSLRKLNFLEPFPSHGNYILCRVTRGDAHDIHRRLYRQGILVRK
                     YGDPELRSFLRVSVGRPEDTDRLMAALRAMAEEL"
     gene            3099..>3332
                     /locus_tag="VFU72_08795"
     CDS             3099..>3332
                     /locus_tag="VFU72_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEU5423623.1"
                     /translation="MTRVGVVQRETRETAVELRLDLDGSGTSEIDTGIGALDHFLSLF
                     ARHGLFDLQVRARGDLHVDPHHITEDVGLCLGQA"
BASE COUNT          534 a         1177 c         1117 g          504 t
ORIGIN      
        1 atttgctcgg tgctgacccg ctcaccgtcg accaccacgc cccggcgcag gcgctccagc
       61 acctcgggcg gcggatggcc atcgagcaac gccaggtact ccttggtcag ctcgaacttc
      121 gggtgggcca cgcggtaggc cagttcacca tcgttggtca aaaggagcag ccccgatgtc
      181 tggcgatcta ggcggcccac cggcaccacc cgctcgggca cctgcaccag gtcgagcacg
      241 gtgcgccgtc cgcgctcatc ctgcgcggtc gtgatgtagc ccgccggctt gttcaacacc
      301 agatagcgct tgcgctgcgg gcggaccagc ttgccgtcga cttcgatccg ctgacgcgcg
      361 ggatcgacgc gcgtgccgag atagcggacg acctcgccgt ccactgtgac ccggccctcc
      421 tggatcagct cctccgcatg gcggcgcgag gcgaccccgg actcggccag aaagcgctgt
      481 agtcgttcca aatccaaatt cgggctcctg cccctcggcg cgggcgtcac ggcgccggcg
      541 tcccgctccg cgcgggatcg ccgctgtaga ctggcagccg caagatcggc tccccgccag
      601 aggagaatag cacgcttccc tgtggcgtcc agccgccgcc cggccgcggc gcgagctgaa
      661 gaacgtagtg cagatcggcg gcctggtccc ggccgaggat caccggcacc accacgccga
      721 acgcgaggtg cttctcggcc aggagctgat tgagcggagc gatgtctccg tcgcgcccaa
      781 gccgcagcca gcggtactgg ccatccagtg cgttgagcag cgcgtcggtg tcggcatagc
      841 ggtcaccgct gccgagcacg accgtgacga tgcgcgagcc gccgcgccag accgcggtga
      901 tcaaacactg gcccgccgcg tcggtggtgc cggtcttgac tccatgcacc cccggctgac
      961 ccagcagctc attggtattc accagggtga tcgaccgggc attgggcccg ccaacctgct
     1021 ccgtctcggt ctgcgcgccg accagttgct ccaggagcgg gtgctgaaag agcgcggcgg
     1081 tggcgatggc cagatcacgc gccgtcgaga cgtggcccgg cgcgtcgact ccatcgggat
     1141 tgaggaagtg gctgtggtcc atgcccagcg aggcggccaa ccggttcatc tcggccacga
     1201 agcgggccat cgggtccgca ccgtcagcag cgggtagccg ctccccggtg gcgcgcgcca
     1261 gcgccagggc cgcgtcgtcg ccggagggca gaagcatgcc gaccaacaga tcgcgcgccg
     1321 tcaccacgtc gcccgtctcc aaccccatgt gcgagtaaat ggccgggtcg accagatcct
     1381 gcggctggac cgtcaccttc gtctccagcg gcaagaatcg aagcgccacc agcgccgaca
     1441 cgatcttcgt cgtactggcg ggaggcagcg gcgtgtccgc gttggcggcg tacaactcga
     1501 ccaggtgcgt cagatcgacg gcaaaagccg cccgcgcgct caattgcacc tggagcgcgg
     1561 gcgccaccgg cctcggggtc gcggccggcg tcggtgtcga agtggcgatc acggcggatg
     1621 gtgcggcggg cgcgtggtcg acgcccaggc gtggtgatgc ccccatgaga gccaaggcag
     1681 ccggcgtgct cgcggccatc gtcggggaag cgatggccgc gttgccggca ggcgcgcatt
     1741 gacagaggac caggagggct ataatcaggg tcagccagcg tggtacaacc gtacgcggcg
     1801 cggctcgagt cagcggcagg ggagccggtc tgcttcgcga gtggtggctc cgcatgacgc
     1861 tccgctccga cacgtgctct gctggcaggg gcaagggcaa gggcatgggc gtacacatgg
     1921 gcgtgggcat cgccttggac gcatgatcga ttctagcatt cagaccgatc ccgactggag
     1981 gcgagcaggt tttggcgagc atcccgaaag cgctacgacg gatcatccgc ccgagcgtcc
     2041 tcgagcagga cggctacgta ccctctgacg acctgctcgc ggtggcgcga gacctggggc
     2101 ggtcggtgga cgagatcagc aagctcgaca ccaacgagaa tccatatggc acctcgatcc
     2161 acgtgcagga ggcgctggca agctttgacc gctaccatcg ctaccccgac gccgaccagc
     2221 gcgccgcccg cggccgcttg gctgagtaca ccggggccgc cgccgagcgc atcatcatcg
     2281 gcaacggcgc cgacgaactg atcgatctct tgatgctcgc cacgatcgac cccggcgacg
     2341 aggtcatcgt gcccgtgccc accttcgggg tctacgcgca gcgcgcggcg ctcttccacg
     2401 gcgaggcccg cgcggtgtcg cgcactgccg actacgaact ggacgtcgat gcgatcgccc
     2461 aagcggtcaa cgagcgcacc aagctgatct tcgtcgcctc acccaacaac ccgacgggga
     2521 atctcgtgac cagccagcaa ctggtgcgcc tgctgcgcac cggcgcgctg gtggtgctcg
     2581 acgaggccta ttacgagttc tcgggcaaga ccttcctgcc gctggtcggc gagtgggaca
     2641 acctggtggt gctgcgcacc ttcagcaagt gggccggact ggccggcttg cgcatcggct
     2701 acggcatctt cccgccggcg ctcgccagcg gcatctggaa ggtcaagcag ccgttcaacg
     2761 tcagcgtcgc ggcgctggcc gcgatcgagg ccgtgctcga cgaccaggag tatctgcgcg
     2821 agcgcgtcag ccgcatccgg gtcgagcgcg ggaggctctt ccgctcgctg cgcaagctga
     2881 acttcctgga gcccttcccc tcgcacggca actacatcct ctgccgcgtc acccgcggcg
     2941 acgcccacga cattcatcgc cggctctacc gccagggcat cctggtccgc aaatacggcg
     3001 atcccgagct gcgcagcttc ctgcgcgtca gtgtcgggcg gcccgaggac accgaccggc
     3061 tgatggcggc gctgcgcgcc atggccgagg agctgtaggt gacgcgcgtc ggcgtcgtcc
     3121 agcgcgagac gcgcgagacc gcggtcgagc tgcggctcga cctcgacggc agcggcacgt
     3181 cggagatcga caccggcatc ggcgcgcttg accactttct gtccctcttc gcgcgccacg
     3241 gcctcttcga tctccaggtc cgtgcccgcg gcgacctcca cgtcgacccg caccacatca
     3301 ccgaggatgt cggcctctgt ctcggccagg cg
//
LOCUS       DASUBI010000460         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_584565, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000460 DASUBI010000000
VERSION     DASUBI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584565"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1364)
                     /locus_tag="VFU72_08800"
     CDS             complement(186..1364)
                     /locus_tag="VFU72_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423624.1"
                     /translation="MPVLRLTPEERVRIAVLAHLLNFQPGFRQAGVSRYIERLLAALP
                     AAAPEDEIVVFAGRGATKATAAFDPALRWLPSRLPTERPGVRILWEQFAAPPALARER
                     IELVHAPVNVAPVLAGRPAVVTVHDLAFLRYPEQYPGTKQRYLRWMTRRSVEHAARVI
                     AVSEHTRRDLLNFYHVAPERVVVVPNGVGPEFRPGDDPAEARALRATHNLPDEFVLFV
                     GTLQPRKNLLGLLRAWARVETAAPLVIVGAPGWQYQPIFDEVRVLGIGERVIFTGYSG
                     DLASWYRAATVFVYPSLYEGFGLPVVEAMACGTPVVTSNVSSLPEVAGDAALMVDPTD
                     QDALAGAIARLLESPELRATLRERGLARAAAFSWERTARETVAVYHSAAGARLAALAR
                     "
     gene            1538..4405
                     /locus_tag="VFU72_08805"
     CDS             1538..4405
                     /locus_tag="VFU72_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5423625.1"
                     /translation="MADPTTLPAEIAQFAALYPFQLDSFQIEAITTFLHGDSVMVAAP
                     TGTGKTIPAEFSVYESFRRGGKVMYTTPIKALSNQKFRDLRALYGEHVGLLTGDVSEN
                     PDAAILVMTTEILRNMLLQTPWELDAVDTIIFDEVHYIADPERGTTWEEAIILCPDHI
                     QLVCLSATVSNAQEIADWISRTHRPIQLITHFERAVPLALYYFLDNKLHLVINHEGEQ
                     VADFPQVGGEVRRRSTQGGLLHDRRQLEDREPAPWEVIRALQGEHMLPAIYFLFSRRD
                     CEEFAQQFAVMRPRLIHDDETAERIEQVIQRYLGGMRLEDRELGQVESIATLARQGIG
                     FHHAGLLPILKQLVEVLFSRGLMQVVFATDTLALGVNMPARTVVVGRMSKWDGRRRRL
                     LISNEFQQMAGRAGRRGMDVKGNVVVPYSPFITFKETLHVATGPLEPVRSSFAIRYNT
                     VLNLWDPPHGSRVRYMLQQSLSQFQAARRVRDLEDEVMLVAREIDEVPQGCLIGLDAG
                     DELLEDYRRLIASINTLRGRERRLQRDKSELQLSLDRRPWPVPGRQALRTVFKTLPAG
                     TPVHLLHGGWAVYLGRGTQGGVGLFLTDDHVVLIAEYRQIDYLPVRGAHVDLPLELTG
                     LDGPVEDVAAMIGPEALEQIWQAVAVLELPDLDTQVAEHERRLEERMGAERRRLEGEL
                     SELREQSHALEEARQQHPCHLCERRKEHQANLRRVATLEKERADLEHDLSRELTGEEE
                     RVRRIIRGIRDVLHRFGYLHRGYATAKADTLANIFDTNGLILAEIVDRGVLDKLEPAD
                     VAEVFSWFAYDRDFRFGNSFTLPGKLVLLRRRLDDLEQEVFAAERGNGLFISTGHSDG
                     FYGAMRAWCHGATMARVLEQIELSEGDLVLTFNKTIDVMRQVREMLVQARPDHPLRDT
                     LAHAEELVRRDIVEQSLTIGFMPVLSAEDARVSLGEDVPAK"
BASE COUNT          772 a         1624 c         1551 g          717 t
ORIGIN      
        1 gtcgccacct gggcacggcc tggtcgaggc tgccagacgg cggcatcgtc gccgatgccg
       61 agcgtgaccg ggctgcccgg tctcgagccc agcgcctggc cgagttgctc gatcagttcg
      121 aactcgccga gctcttcgat cggcctaccg ccggtcggaa tccgcattgc ctgtaacact
      181 tctctctaac gtgccagcgc ggccagccgc gcgccggccg cgctgtgata gaccgctacc
      241 gtctcacggg ccgtccgttc ccaactgaag gccgccgccc gcgcgaggcc acgttcgcgc
      301 agggtggcac gcagctcggg gctctccagc agccgcgcga tcgcgccggc cagagcatcc
      361 tggtcggtgg gatcgaccat gagcgcggcg tcgccggcca cctccggcag cgaggagacg
      421 ttcgaggtca cgactggcgt gccacaggcc atcgcctcga cgaccggcag gccgaagcct
      481 tcgtagagcg acgggtagac gaagacggtc gccgcgcggt accaggacgc caggtctccg
      541 gaatagccgg tgaagatgac tcgctcgccg atgccgagga cgcgcacctc gtcgaatatc
      601 ggctggtatt gccagccggg cgcgccgacg atcaccagcg gcgcggcggt ctcgacccgc
      661 gcccaggcgc gcagcagccc gagtaggttc ttgcgcggct ggagtgtgcc gacgaagagg
      721 acgaattcgt ccggcaggtt gtgcgtcgcc cgcagcgccc gcgcctccgc cggatcgtcg
      781 cccggccgga attcaggacc aacgccgttc ggcaccacca ccacgcgctc cggcgcgacg
      841 tggtagaagt tcaagaggtc gcgccgggtg tgctcggaga ccgcgatcac ccgcgcggcg
      901 tgctcgaccg agcgccgggt catccagcgc aggtagcgtt gcttggtgcc ggggtactgc
      961 tccgggtagc gcaggaacgc caggtcgtgc acggtgacga ccgccggccg gccggccagc
     1021 accggcgcga cgttgaccgg cgcgtgcacc agttcgatgc gctcgcgcgc cagcgccggc
     1081 ggcgcggcga actgttccca gaggatgcgg accccggggc gctcggtcgg cagccggctc
     1141 ggcagccagc gcagggccgg gtcgaacgcc gcggtcgctt tggtcgcgcc gcgtccggcg
     1201 aagaccacga tctcgtcctc gggcgcggcg gccggtaagg ccgccagcag gcgctcgatg
     1261 tagcggctca ccccagcctg gcggaaaccg ggctggaaat tcaagagatg ggccaaaacg
     1321 gcgatgcgca cgcgctcctc cggcgtcaat cgtagcacag gcatccgccg tgcttccttg
     1381 cgtagtcggg gcacgagccc cgctgccttg tcacctccgc attgcaaacg cacggttatg
     1441 tcatgaaggg caggcgatca atgctatact gcgcgtggcc ggcacagagc ggccggcgcc
     1501 aggggacagg agtagcgttg gaagcgacgg atcattgatg gccgacccta ccactcttcc
     1561 cgctgagatc gcccagttcg ccgcgctcta tccttttcaa ctggactcct ttcaaattga
     1621 agcgatcacg accttccttc acggcgattc ggtcatggtc gcggctccca ccggcacggg
     1681 caagacgatc ccggctgaat tcagcgtcta cgaaagcttc cggcgcggtg gcaaggtcat
     1741 gtacaccacg ccgatcaagg cgctgagcaa tcagaagttc cgcgacctgc gtgccctcta
     1801 cggcgagcac gtcggcctgc tgaccggcga cgtcagcgag aacccggacg ccgccatcct
     1861 ggtgatgacg accgagatcc tgcgcaacat gctcctgcag acgccctggg agctggacgc
     1921 cgtcgacacc attatcttcg acgaggtcca ctacatcgcc gacccggagc gcggcaccac
     1981 ctgggaagag gcgatcatcc tctgccccga ccatatccaa ctggtctgcc tctcggccac
     2041 cgtctccaac gcgcaggaga tcgccgattg gatcagtcgc acccaccgcc cgatccagtt
     2101 gatcacccac ttcgagcgcg ccgtgccgct ggccctctac tacttcctgg acaacaagct
     2161 gcacctggtg atcaaccacg agggcgagca ggtggccgac ttcccgcagg tcggcggcga
     2221 agtgcggcgg cgctcgacgc agggcggact gctgcacgac cggcgccagc tcgaggatcg
     2281 cgagccggcg ccctgggaag tgattcgcgc actccagggc gagcacatgc ttccagcgat
     2341 ctacttcctc ttcagccggc gcgactgcga ggagttcgct cagcagttcg cggtcatgcg
     2401 gccgcgcctg atccacgacg acgagaccgc cgaacgcatc gagcaggtca tccagcgcta
     2461 cctgggcggg atgcggctgg aggaccgcga gttgggccag gtcgaatcga tcgcgaccct
     2521 ggcgcggcaa gggatcggct tccaccacgc cgggctgctg ccgatcctga aacaactggt
     2581 cgaggtgctc ttcagccgcg ggctgatgca agtcgtcttc gctaccgaca cgctagcgct
     2641 gggcgtcaac atgcccgcgc gcacggtggt cgtgggtcgc atgtcgaagt gggacggccg
     2701 ccggcgccgg ctgctgatca gcaacgagtt ccagcagatg gcgggacgcg cgggtcggcg
     2761 aggcatggac gtcaagggca acgtagtcgt cccctacagc ccctttatca ccttcaagga
     2821 aacgctgcat gtcgccaccg gcccgctgga gccggtgcgc agctcctttg ccattcgcta
     2881 caacacggtg ctcaacctct gggacccgcc ccacggcagc cgcgtacgct acatgctgca
     2941 gcagagcctg tcgcagtttc aggccgcgcg gcgcgtgcgc gacctggagg acgaggtcat
     3001 gctggtcgct cgcgagatcg acgaggtgcc gcagggctgc ctgatcgggc tcgacgccgg
     3061 cgacgagctg ctggaggact accgccgcct gatcgcctcg atcaatacgc tgcgcggccg
     3121 cgagcggcgt ctccagcgcg acaagagcga gttgcagctt tccctcgacc ggcggccctg
     3181 gccggtgccc ggccgccagg cgttgcgcac cgtcttcaaa acgctccccg ccggcacgcc
     3241 ggtccacctg ctgcacggcg gctgggccgt ctacctgggg cgcggcacgc agggcggcgt
     3301 cggcctcttc ctcaccgatg accacgtcgt gctgatcgcc gagtatcgtc agatcgacta
     3361 cctgccggtg cgtggcgcgc acgtcgacct gccgctcgag ctaaccgggc ttgacggtcc
     3421 ggtggaagac gtggcggcga tgatcgggcc cgaggcgttg gagcagatct ggcaggcggt
     3481 cgcggtgctg gagttgccgg acctcgacac ccaggtggcc gagcatgagc ggcgcctgga
     3541 ggagcgcatg ggtgccgagc gccgcaggct ggagggtgag ctgagcgaac tgcgcgagca
     3601 atcgcacgcg ttggaagagg cccgccagca gcacccctgc cacctctgtg agcgccgcaa
     3661 ggagcaccag gccaacctgc gtcgagtcgc cacgctggag aaagagcgcg ccgacctgga
     3721 gcacgacctg agccgcgagc tgaccggcga ggaggagcgg gtgcgccgca tcattcgcgg
     3781 catccgcgac gtgctgcatc gcttcggcta tctgcaccgc ggctacgcca ccgccaaggc
     3841 cgacacgctg gccaacatct tcgacaccaa cggattgatc ctggccgaga tcgtcgaccg
     3901 cggtgtcctc gacaagctcg agcccgccga cgtggccgag gtcttctcct ggttcgccta
     3961 cgaccgcgac ttccgcttcg gcaactcgtt caccctgccg ggcaagctgg tgttgctgcg
     4021 ccgtcgcctg gacgacctgg agcaggaggt cttcgccgcc gagcgcggca acggcctctt
     4081 catctcgacc ggccacagcg acggcttcta cggcgccatg cgcgcctggt gccacggcgc
     4141 caccatggcg cgtgtgctgg agcagatcga gctgagcgag ggcgatctcg ttctgacctt
     4201 caacaagacg atcgacgtga tgcgccaggt ccgcgagatg ctggtccagg cccgacccga
     4261 tcacccgttg cgcgatacgc tcgcccacgc cgaggagctg gtgcgccgcg acatcgtcga
     4321 gcagtcgctc accatcggct tcatgcccgt gctgagcgcg gaggatgccc gggtgagcct
     4381 gggcgaggat gtgccggcca agtaggacgg cgcacgctgc ccgtcgaata cgtctgctgc
     4441 cgggtgagga cggacccttc gactgcgctc aggctgacag ggagcgggct gaggtctatc
     4501 ggacgaggcg agacctcatc gtcagcccct ctctcaccct gtcagcccga gcgcagtcga
     4561 gagatccgtg ccccaggcgc cacccaatct acgcgaccgt cccgatctct actgcgcgct
     4621 ctccaaatac ttcagtaccg acgaattcac gtcgtcgccc atgg
//
LOCUS       DASUBI010000461         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_231172, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000461 DASUBI010000000
VERSION     DASUBI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231172"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFU72_08810"
     CDS             complement(<1..764)
                     /locus_tag="VFU72_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423626.1"
                     /translation="MANEPEDHTRSRSISSTGLRQRAGTSFNRTFASFSNYNYRLYWF
                     GQLVSVIGTWISRVAQAWLVLKLTNSSFDLGLVGTLQFLPLTAFALFGGVLADRFPKR
                     RLLVCTQSVMALQSLALALLITSGRIQIWHIYVLAMVLGLATAFDNPTRQAFVSEMVG
                     TDNLSNAVALNSSLFNTARIIGPAIGGFLIAAFSISVPFYVNAVSFLAVIAGLLLMRP
                     AEFHNVPKPSRGPVLRRMREGISYAARTPEVAVVLIV"
     gene            complement(825..>3779)
                     /locus_tag="VFU72_08815"
     CDS             complement(825..>3779)
                     /locus_tag="VFU72_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423627.1"
                     /translation="REVAQHGIGGLASANLGLFDEGQAAILARSAAGHGAAAVEPAIL
                     DQVNVVLPARQQLQAAVSLLAVPAPIPHAMGQLRTVDGHPVDFSALGPNEIVLNTDAA
                     ALFGINGGEPVQIVRHGETWDVRVAAVVRDGGLGGLDPVVLVPLPAYQAHIDAPGQIN
                     AVLATNGGGVDAVARSDQVSQALRLPLADQEAARRIHDFLHQPEVQRTILAAEGQEQG
                     RDRARIEALRLEAAKPEVSERFISLISAPRTRAELSQLALIAPGGQAIFGPLRQLSPL
                     TVLDIKQEALNQATDFGGVVTTAFLVLGIFSIAAGLILIFLIFQLLAADRLAELATLR
                     ALGMSRRQILAMFLFEGLLYDLLGAALGALFGLAATLLTTRALASALASFGFTLRWHV
                     APRSLLLAFSLGALLTFATMAFAAWRASQAGIVAATRGEARDESRAWLLGLGALLMIA
                     GGLVWWRWHAPLYAFEPRSPLVLPGALTLALLGLAVLLTGAAGVAAKRLRPRAARRLE
                     LTAGAGATLLGFALAGVWARSLVALPTPGGDLAHDALTIGVGGVVLVLAATWAATRAV
                     GPALRLLDRALAPLAALRAVARPAIGALNYQRWRTGLAVVMFGMVIFIMVTALVMISG
                     LLGAYSEGEPPVAGFAIQAQTGQPLDLPRALTSASAVSDKSFSAVGGTAALDVQAVQL
                     GAPRGGWLSETLVTVDDGFLEGIKTNLVRRAPGYDGNGSVWHALRQHPGTAVVSPTLV
                     GSSGLTVPPVKNFAPLTLWVRPDDGGQPLKLTVIGVIDSRSQLPPGIYTSRATAAGMG
                     VALPAPSTYYLQPRASVHAADAITGLRVSFAGKGLAVMALSDQVQTAQSIRLLLTRIV
                     QGFMGLGLIAGVAALGMLGVQAVIERRAQLGTLRALGFTRWQSRATLAFESAVIALLG
                     VALGVGLGLILSHSLVALLGTDHPELHYVIPWGQIALTAALALAGSALASAVAIWQAG
                     RVSPADALRAGD"
BASE COUNT          589 a         1327 c         1399 g          464 t
ORIGIN      
        1 acgatcagca ccaccgctac ctcgggcgtg cgcgcggcgt agctgatacc ctcgcgcatg
       61 cgccgcagca ccgggccgcg gctcggcttg ggtacgttgt ggaactcggc cgggcgcatc
      121 aggagcagcc cggcgatcac cgccaggaag ctgactgcgt tgacgtagaa cggcacgctg
      181 atgctgaagg cggcgatcag gaagccgccg atggccgggc cgatgatgcg cgccgtgttg
      241 aagaggctgg agttgagcgc gacggcgttg gacaggttgt cggtccccac catctcgctg
      301 acgaacgcct gccgcgtcgg gttgtcgaag gccgtggcca gcccaagcac catcgccagg
      361 acgtagatgt gccagatctg gatccggccg ctggtgatca ggagcgccag cgccagcgac
      421 tgcagcgcca tcaccgactg ggtgcagacc aacaggcggc gcttggggaa gcggtcggcc
      481 agcacgccgc cgaagagggc gaaggccgtc aggggcagga actggagcgt gccgaccagg
      541 ccgaggtcga aggaggagtt ggtcagcttg agcaccagcc aggcctgggc cacgcgcgag
      601 atccaggtac cgatcaccga aacgagctgc ccaaaccagt agaggcgata gttgtagttg
      661 ctgaaggagg cgaaggtgcg gttgaaggag gttccggccc gctgccgcag cccggtggat
      721 gagatgctcc tcgacctggt atggtcctcc ggttcgttcg ccatagcggc aatcatacgg
      781 caagacgcgg ggaatggatc atgccgcgat gaacccgaac gagctcagtc gcccgcccgc
      841 agcgcgtcgg cgggcgacac acggcccgcc tgccagatgg ccaccgcgct ggccagcgcc
      901 gagccggcca gcgccagcgc ggcggtgagc gcgatctggc cccaggggat gacataatgt
      961 aactcgggat ggtcggtgcc cagcagcgcg accaggctgt gcgagaggat cagccccagg
     1021 ccgaccccca gcgccacccc gagcagggcg atcaccgccg actcgaaggc cagcgtggcc
     1081 cggctctgcc agcgggtgaa gccgagcgcg cgcagcgtgc cgagctgcgc ccgccgctcg
     1141 atgaccgcct gcacgcccag catgcccagc gccgccacgc ccgcgatcag ccccagcccc
     1201 atgaagccct gcacgatgcg agtcaggagc agccggatcg actgcgccgt ctgcacctgg
     1261 tcgctcaacg ccatgaccgc gaggcccttg ccggcgaagg agacgcgcag cccggtgatc
     1321 gcgtcggccg cgtgcacgct cgcgcgcggc tgcaggtagt aggtgctcgg cgcgggcagc
     1381 gctacgccca ttccggccgc ggtggcgcgc gaggtgtaga tgccgggcgg gagctgcgag
     1441 cgcgagtcga tcaccccgat gaccgtcagc ttgagcggtt ggccgccgtc gtcgggccgc
     1501 acccaaagcg tcagtggggc gaagttcttg accggcggca cggtcagccc gctgctgccg
     1561 accagcgtgg ggctgaccac ggcggtgccg gggtgctggc gcagcgcgtg ccagacgctg
     1621 ccattgccgt cgtagccggg ggcgcggcgc accaggttgg tcttgatccc ttccagaaag
     1681 ccgtcgtcga ccgtcaccag cgtctcactc agccagccgc cgcgcggggc gccgagctgt
     1741 accgcctgca cgtcgagtgc ggccgtgccg ccgacggcgc tgaagctctt gtccgagacg
     1801 gcgctggcgc tggtgagcgc ccgtggcaga tcgagcggct ggccggtctg ggcctggatg
     1861 gcgaagccgg ccaccggcgg ctcgccctcg ctgtaggcgc cgagcagccc actgatcatg
     1921 accagcgcgg tgaccatgat gaagatcacc atgccgaaca tcaccaccgc cagcccggtg
     1981 cgccagcgct ggtagttgag cgcgccgata gccgggcgtg cgaccgcccg cagcgccgcc
     2041 agcggagcca gcgcgcggtc gagcaggcgc agcgccgggc cgaccgcgcg cgtggccgcc
     2101 caggtggcgg ccagcaccag cacgacgccg ccgactccga tggtcagcgc gtcgtgcgcg
     2161 agatcgccgc ccggcgtcgg cagcgcgacc aacgaccgcg cccagacgcc ggccagggcg
     2221 aagccgagca gggtcgcgcc cgctccggcc gtcagctcca ggcggcgcgc ggcccgcggc
     2281 cggaggcgct tggcggccac gccggccgcg ccggtcagca gcaccgccaa cccgagcagc
     2341 gccagcgtca gcgcgccggg caataccagc gggctacgcg gctcgaaggc gtagagcggg
     2401 gcgtgccagc gccaccagac cagtccaccc gctatcatga ggagggcgcc gagcccgagc
     2461 agccaggcgc ggctctcgtc acgcgcctcg ccgcgcgtgg ccgccacgat gccggcttgg
     2521 ctggcgcgcc aggcggcgaa ggccatggtg gcgaaggtca gcagcgcgcc caggctgaag
     2581 gccaggagca ggctgcgcgg cgcgacgtgc cagcgcaggg tgaagccgaa gctggccagc
     2641 gccgaggcga gcgcgcgcgt cgtcagcagg gtggccgcca gcccgaagag cgcgccgagc
     2701 gccgcgccga gcaggtcgta gagcagcccc tcgaagagga acatggcgag gatctggcgg
     2761 cgcgacatgc ccagtgcgcg cagcgtcgcc agctcggcca gccggtcggc ggccagcagt
     2821 tggaagatca ggaagatcag gatcaggccg gcggcgatcg agaagatgcc gagcaccagg
     2881 aaggcggtgg tgacgacgcc gccgaaatcg gtggcctggt tgagggcctc ctgcttgatg
     2941 tcgagcacgg tgagcgggct gagctgccgt agcgggccga agatggcctg gccgccgggg
     3001 gcgatcagcg ccagttggct cagctcggcg cgggtgcgcg gcgccgagat caggctgatg
     3061 aagcgctcgc tgacctccgg tttggcagcc tccaaccgca gcgcctcgat gcgggcccgg
     3121 tcgcgcccct gctcctgccc ctcggccgcc aggatggtgc gctgtacctc gggctggtgc
     3181 aggaagtcgt ggatgcggcg tgcggcctcc tggtcggcca gcggcaggcg cagtgcctgg
     3241 ctgacctggt cgctgcgcgc caccgcgtcc accccgccgc cgttggtcgc cagcaccgcg
     3301 ttgatctgcc ccggcgcgtc gatgtgcgcc tggtaagcgg ggagcggcac cagcacgacc
     3361 gggtcgagcc cgcccaggcc cccgtcgcgc accaccgccg ccacgcgcac gtcccaggtc
     3421 tcgccgtgcc gtacgatttg caccggctcg cccccgttga tgccgaagag cgcggccgcg
     3481 tcggtgttga gcacgatctc gtttggcccg agcgcgctga agtcgaccgg atggccgtcg
     3541 acggtgcgca gttgccccat cgcgtgcggg atcggcgccg gcacggcgag cagcgagacc
     3601 gccgcttgga gctgctgccg cgcgggcagg acgacgttga cctgatcgag gatggccggc
     3661 tcgacggccg ccgcgccgtg gcccgcggcg gacctggcca gaatcgccgc ctgtccctcg
     3721 tcgaacaggc cgaggttcgc cgaggccaag ccgccgatgc cgtgctgcgc cacctcgcg
//
LOCUS       DASUBI010000462         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_927259, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000462 DASUBI010000000
VERSION     DASUBI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927259"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..426)
                     /locus_tag="VFU72_08820"
     CDS             complement(64..426)
                     /locus_tag="VFU72_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423628.1"
                     /translation="MNTRSVTAEEIAENVASIEAVPQELWASGHDRTFALHWARRGYD
                     CYAYRAVGGIWNLWLEDRRIRLDGPPSRARLDELQRMRARITVERQEARDSEQVWERW
                     RLAVAEHRAQQARQGREG"
     gene            complement(460..684)
                     /locus_tag="VFU72_08825"
     CDS             complement(460..684)
                     /locus_tag="VFU72_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423629.1"
                     /translation="MTREEAAQLKPGDMLEYELSLEAEGYGVLVRRYVLSEFVKPSKN
                     SRRVVIRPMFREEGWNRARAVGIDKLRKWG"
     gene            complement(681..1160)
                     /locus_tag="VFU72_08830"
     CDS             complement(681..1160)
                     /locus_tag="VFU72_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423630.1"
                     /translation="MSTYEHLPCLSVWCAGHPVSVNHAYGSGGYGRSRWRYLQAEARC
                     WRDAVRDETIKARLSARENPLRAAAYRLPLRVVCTFYGTRADADNLLKLTVDGLKAGL
                     GIDDKHYAQIEAEKQPRFLVPDKRQFFTGVLISVWASAADAADSVSAMREREEARTA"
     gene            complement(1157..1468)
                     /locus_tag="VFU72_08835"
     CDS             complement(1157..1468)
                     /locus_tag="VFU72_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423631.1"
                     /translation="MELRRDELTPAEKTLMFRLMAHRSHALHDPAERCAKARAGMEKR
                     FWRQAQELHPGASEVTLARVAEQLRKAYYTELALKSHMARRTKSAASASASGKARRSA
                     A"
     gene            complement(1459..1830)
                     /locus_tag="VFU72_08840"
     CDS             complement(1459..1830)
                     /locus_tag="VFU72_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423632.1"
                     /translation="MLTVSWVLLVVGLVVAAFVGSAGMLLALSLVSMKRQPVLEPPYD
                     DDEDDEGWGGDYASIDGPTEPVPPPAPAVVRTAASVRAQVWAPTIGRTPRPWETGPLE
                     LELDHLHTGVEEIEEGPGLWN"
     gene            complement(1838..2185)
                     /locus_tag="VFU72_08845"
     CDS             complement(1838..2185)
                     /locus_tag="VFU72_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423633.1"
                     /translation="MATAKSATGTRSGTQWQAALERYRQAEQARRAALARAAKLRARA
                     KAMEEDIEDLRYCVAQAAEARGIDDDHVLDGYELIADLEERQAKLRREAYALEQRYAA
                     PEAVAAAAAERGA"
     gene            complement(2221..2505)
                     /locus_tag="VFU72_08850"
     CDS             complement(2221..2505)
                     /locus_tag="VFU72_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423634.1"
                     /translation="MRVVDEGKPELTVEERRRQALRPIFAYLDQWGIKRTWLAARLGV
                     SRSLLWKYEHGWCSEPADFVDRACAALGRTREWLGLPAAAKASQRQKRAS"
     gene            2789..3322
                     /locus_tag="VFU72_08855"
     CDS             2789..3322
                     /locus_tag="VFU72_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423635.1"
                     /translation="MPRPWAYADNIEETPFLKLLEDYMYSQRPPITAAQLSYKTKIPS
                     TTIIGWFREGRVPQPRTLQRIAAGTTIPLADLFTACGYPVPASVESGPADPFEAMIER
                     ALHDPRFSSAAREALVARIREVQSGALAAPGERYIAAEHEQVVEGTTSPAHPAIQPPA
                     TPRSRPGARPRTRTEAR"
     gene            3696..4883
                     /locus_tag="VFU72_08860"
     CDS             3696..4883
                     /locus_tag="VFU72_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423636.1"
                     /translation="MFRRNRQTAGRQVAARPSAIPPHRQRANDYQIISYPDQLARWLR
                     QVNADMSGVQLGELARRPDYGELLWQARTDGEAIRRKAATALYRKYGYAVTPDPRRPI
                     PFSPASLFYEAFAKVGIQRQNILPVTGSNGLKALQVDLSESLKHSVFGWIFTYPDRRP
                     PDVMVDLNAPVFVMMASPDSDGPYGDDELPRKVELPVWLIHASLCLWVLLQNWGQHTG
                     PVTPGGQVIVPPFTHIEPNAVPIAHMFVKALYGLGTFCTPDFGCPCNRVIHLLRTPPP
                     RHPEMTGTQPPWPYGRRPQQPQHTSHPPQRPGTVNTAPPPAPDARYQRPPGHPAAPSR
                     PLQPGERLYPTPPTPPLGTEAGAGPGATSGERNQRDLLRDLGLDDPYDMDLPNGWDDD
                     DEQ"
     gene            4873..5319
                     /locus_tag="VFU72_08865"
     CDS             4873..5319
                     /locus_tag="VFU72_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423637.1"
                     /translation="MSSDSGDHAMTELEHLARRTRLTRLERPLEELMTQTRRDLARAL
                     LVAREHASHPYIAQLEQENAALAAEVAQLRAELAGEHRDRRDLTKMVTQMLDIVRAVA
                     TARRLYSQRPGGTGEDIHLIPHSLVVRATELLTIPPGDRDTGTDTQ"
BASE COUNT          824 a         2246 c         1509 g          874 t
ORIGIN      
        1 gcctccgcca cacgctcggc aaccgtcacg tactccttgc cgtgcagatt cactggcata
       61 gcgctagccc tcccgtccct gccgcgcctg ctgtgcgcgg tgctccgcga ccgccagccg
      121 ccagcgctcc cacacctgct cgctgtcccg cgcctcctgc cgctccaccg tgatccgcgc
      181 ccgcatccgc tgcagctcgt ccagcctcgc ccgcgagggc ggcccgtcga gccggatgcg
      241 ccgatcctcg agccacagat tccagatgcc gccgaccgcg cgatacgcat agcagtcgta
      301 gccccgccgt gcccagtgca gcgcgaacgt ccgatcatgc ccgctcgccc acagctcctg
      361 cggcaccgcc tcgatgctcg ccacgttctc ggcgatctcc tctgccgtca ccgatctcgt
      421 gttcatgccg tcctcgtttc ctctcgattg ctcgcggcat cagccccact tgcgcaactt
      481 gtcgatgccg actgcccgcg cccgattcca gccctcttct cgaaacatcg gccggatcac
      541 cacacgccga gagttcttgc tcggcttgac gaactccgac agcacgtagc gcctgaccag
      601 cacgccgtat ccctcggcct ccaggctcag ctcgtattcg agcatgtcgc ccggcttgag
      661 ctgcgccgcc tcctctcgtg tcatgccgtc ctcgcttcct ctcgctcgcg catagcgctc
      721 acgctgtccg ctgcatccgc agccgaggcc cacacgctga tcagcacgcc cgtgaagaac
      781 tgccgcttgt caggcaccag gaaccgcggc tgcttctccg cctcgatctg cgcatagtgc
      841 ttgtcgtcga tccccaggcc cgccttcagt ccgtccaccg tcagcttcag caggttgtcc
      901 gcatcggcgc gcgtgccgta gaacgtgcac actacccgca gcggcagccg atatgcagcg
      961 gcgcgcagcg gattctcccg tgcggagagg cgcgccttga tcgtctcgtc gcgcaccgcg
     1021 tcacgccagc accgcgcctc cgcttgtaga tagcgccacc gtgagcgccc gtagcctccg
     1081 ctgccgtacg cgtgattcac cgacaccgga tgccccgcgc accacaccga caggcagggc
     1141 agatgctcat aggtgctcat gccgcgctcc tcctggcctt gccgctcgcc gatgcgctgg
     1201 ccgcgctctt cgtccgccgc gccatgtggc tcttgagcgc gagctcggtg tagtacgcct
     1261 tgcgcagctg ctccgccacc cgcgccagcg tgacctcgct cgcgccgggg tgcagctcct
     1321 gcgcctggcg ccagaagcgc ttctccatcc ccgcccgcgc cttcgcacag cgctccgcgg
     1381 ggtcatgcag cgcgtgcgag cggtgcgcca tcagccgaaa catcagcgtc ttctccgccg
     1441 gcgtcagctc atcgcgtctc agttccatag tcccggcccc tcctcgatct cctcgacccc
     1501 cgtgtgcagg tggtccagct ccagctccag cggccccgtc tcccacggcc gtggcgtgcg
     1561 accaatcgtc ggcgcccaga cctgcgcgcg caccgaggcc gcagtgcgca cgacggccgg
     1621 cgctggtggc ggcaccggct ccgtcggccc atcgatgctc gcatagtcac ccccccagcc
     1681 ctcgtcgtcc tcatcatcgt cgtacggcgg ctccagtacc ggctggcgct tcatcgacac
     1741 cagcgacagt gccagcagca tccccgccga gcccacgaac gccgccacca ccagccccac
     1801 caccagcagc acccagctca ccgtcagcat cgtcccctca cgctcccctc tctgccgccg
     1861 ccgccgccac cgcctccgga gccgcatacc gctgctccag cgcgtacgcc tcacgccgca
     1921 gctttgcctg ccgctcctcc aggtccgcga tcagctcgta gccgtcgagc acgtggtcgt
     1981 cgtcgatccc ccgcgcctcc gccgcctgcg ccacgcagta gcgcagatcc tcgatgtcct
     2041 cttccatcgc cttcgcccgt gcccgcagct tcgccgcccg tgccagcgcc gcccgccgtg
     2101 cctgctccgc ctgccggtag cgctccagcg ccgcctgcca ctgcgtcccc gatcgtgtgc
     2161 ccgtcgcgct cttcgccgtc gccatcgtcg tcgctcctct cgatccgctc tcgctcgccc
     2221 ctagctcgcg cgcttctgtc gctggctcgc cttcgccgcc gccggcagtc cgagccactc
     2281 acgcgtccgg cccagcgccg cacacgcccg gtctacgaag tccgccggct cactgcacca
     2341 gccgtgctcg tacttccaca gcagcgaccg gctcactccg agcctggccg ccagccatgt
     2401 ccgcttgatg ccccactggt cgagataggc gaagatcggc cgcagcgcct gcctacggcg
     2461 ttcctccacc gtcagctcgg gcttgccctc atccaccact cgcatctcgc cattcctcct
     2521 tccgcggctc atatctgccg cgtcacttct gtgtcgtagt atgccaccgt ccgcgacagt
     2581 tgtcaacctc tcgcggtggt aaaatatatt tataggttgg catggcacta taaaggtgaa
     2641 cttattctta cccagggcgg tccgcgagca ccttgtgcgg gactatggca ccgcgcagta
     2701 tggtagactc gatgcagaga gccgcgcccc accggctgcc gtccgcgccg ccagtccagc
     2761 cgtcagatca cgagaggatc gcctgcctat gccacgccca tgggcctacg ccgacaacat
     2821 cgaagagacg cccttcctca agctcctcga ggattacatg tactcccagc gcccacccat
     2881 caccgccgct cagctctcct acaagaccaa gatccccagc accaccatca tcggctggtt
     2941 ccgcgaaggc cgtgtgcccc agccgaggac cctccagcgc atcgccgccg gcaccaccat
     3001 ccccctcgcc gatctcttca ccgcctgcgg ctatcccgtc cccgcatccg tggagtctgg
     3061 gcccgccgat cccttcgagg cgatgatcga gcgtgccctg catgatccgc gcttctcatc
     3121 cgccgcccgc gaggccctcg tcgctcgcat ccgtgaggtc cagtccggcg cgctcgccgc
     3181 ccctggcgaa cgctacatcg ccgccgagca cgagcaggtc gtcgaaggca ccacctcgcc
     3241 cgcccacccc gccatccagc cgcccgccac cccgcgctcc cgcccaggcg cccgcccacg
     3301 tacccgtacc gaagcgaggt aggcaaccgc acgcagaagt tacgcataat tgcctaggct
     3361 acccctcccc cccaataccg ctgtgcttgc ttgacgctcg cttgtgcacg gagtagagtg
     3421 cggcacacgc gcgtatcacg ccgtgagaat gttcaacgcc gagcgagaga cgatggggca
     3481 cgcggccgcg tcggggcgaa gaggaggtct ctgccagtcc ggcgcgaccg gcgcatgcta
     3541 cactggaatg gaagagcacg gggtggcgca gggaatgtga gcgccagcag gatcgtggag
     3601 tgcggacata ctatgcgggc tgcggctagg tctcgtcgtc ggagcttaca cgagtgccgc
     3661 tcgacccgcg cgcagggaga ggacgtgtac cgcctatgtt ccgtcgcaac cgtcagaccg
     3721 ccggccggca ggtcgccgct cggcccagcg cgataccacc gcatcgtcag cgtgctaacg
     3781 actaccagat catcagctac cccgaccagc tggcccgctg gctccgccag gtcaatgccg
     3841 atatgagcgg cgtccagctc ggtgagctcg cgcgccgacc cgattacggc gagctgctct
     3901 ggcaggcccg caccgatggc gaagccatcc gccgcaaggc cgctacggcg ctctatcgca
     3961 agtacggcta cgccgtcacg cccgacccgc ggcggcccat cccgttctca cccgcctcgc
     4021 tcttctacga ggccttcgcc aaggtcggca tccagcgcca gaacatcctg cccgtcaccg
     4081 gcagcaacgg cctcaaagcg ctgcaggtcg atctgtccga gagcctcaag cacagcgtgt
     4141 tcggctggat cttcacctac cccgaccgcc ggccgcccga cgtgatggtc gacctcaacg
     4201 cgccagtgtt cgtgatgatg gcctcccccg actccgacgg gccctacggc gacgacgagc
     4261 tgccgcgcaa ggtcgagctg cccgtctggc tcatccacgc cagcctctgc ctgtgggtgc
     4321 tgctgcagaa ctgggggcag cacaccggcc ccgtcacccc cggcggccag gtcatcgtgc
     4381 cgcccttcac ccacatcgag cccaacgccg tccccatcgc ccatatgttc gtcaaagcgc
     4441 tctacggcct cggcacgttc tgcactcctg acttcggctg cccctgcaat cgcgtcatcc
     4501 acctgctgcg caccccgccg ccgcgtcacc ccgagatgac cggcacgcag ccaccctggc
     4561 cctacggccg ccgcccacag cagccgcaac acacgtcaca tcccccgcaa cgtcccggca
     4621 ccgtcaacac cgccccaccg cctgcgcccg atgcgcgcta tcagcgtcca cccggccacc
     4681 ccgccgcccc ctcgcgtccc ctgcagcccg gcgagcgcct ctaccccacg ccccccacac
     4741 caccgctcgg gaccgaggct ggtgctggcc ctggcgccac ctccggcgag cgcaaccagc
     4801 gcgacctcct gcgcgacctc ggcctcgacg acccctacga catggacctg ccgaacggct
     4861 gggacgacga cgatgagcag tgatagcggt gatcacgcga tgacagagct tgagcatctc
     4921 gcgcgccgca cgcgactgac gcggctggaa cggccgctcg aagagctgat gacgcagaca
     4981 cggcgtgacc tcgcccgcgc gctgctggtg gcgcgcgagc acgcctcgca tccatacatc
     5041 gcgcaactgg aacaggagaa tgcagcgctc gccgcggagg tcgcgcagct gcgtgccgag
     5101 ctcgccggcg agcaccgcga ccgccgtgac ctcaccaaga tggtcacgca gatgctcgac
     5161 atcgtgcgcg ccgtcgccac cgcccgccgg ctctatagcc agcgccccgg cggcacaggt
     5221 gaggacatcc acctcatccc gcacagcctc gtcgtccgcg ccaccgagct gctcaccatc
     5281 ccgcctggcg atcgcgacac cggcaccgac acacaataag ggggagggct aatccctccc
     5341 ccgcttcccc ccaccaccgc catccgtcag attgtccagc ggcgagtgca gccggtgctc
     5401 gtcgagcgcg cgttgatgcg ccgcgctcgc cccgtagcgg tccagcatcg tcc
//
LOCUS       DASUBI010000463         7254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_314877, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000463 DASUBI010000000
VERSION     DASUBI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314877"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFU72_08870"
     CDS             complement(<1..304)
                     /locus_tag="VFU72_08870"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEU5423638.1"
                     /translation="MDEPGWMHRFLNSTRGRIVALLRRSSRTVDELAQELELTDNAVR
                     SHLATLERDGLVVQQGFRPGARKPAYEYQLTDEAEQLFPKPYAAILDQLLGVLAGRL"
     gene            473..898
                     /locus_tag="VFU72_08875"
     CDS             473..898
                     /locus_tag="VFU72_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423639.1"
                     /translation="MNLATLIVWLVTAVGGLILVGIFLSRGGLQTGREHRFPRPLPFA
                     HALLAVASLVLFLIWWANGSDALKPIVLVGLLLTAVLGIWMFVLWLGKLRVRLSPSVG
                     AATTRPAEDSFPTVLVALHGILAVSTLVLYIVAAFIVAS"
     gene            931..1467
                     /locus_tag="VFU72_08880"
     CDS             931..1467
                     /locus_tag="VFU72_08880"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5423640.1"
                     /translation="MANPLRRFAGDPVHPATVHFPLALFPAATLFDVLALTLGDGSVY
                     TRGAFILIAAASVAALLAMTTGFVQLLDVPPESRAWRLALWHMSVQLLAASVLVTGLL
                     LRLGHLDAAHAPIAAIALNAGGSAILLFGGWLGGHMVFSHGVSVEAGADTLFAEAPTP
                     TPIAQPLELHERDIAARR"
     gene            1670..3058
                     /locus_tag="VFU72_08885"
     CDS             1670..3058
                     /locus_tag="VFU72_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEU5423641.1"
                     /translation="MHAPGAVLLVSCYELGHQPLSLAFPLARLRQAGFEPGAVDTALD
                     TLSDEAIDAARLVAIAVPMHTALRLGAEIAARVRRRNASAVICCFGLYAWLNAETLLA
                     DTADYVIGGESEEPLVALAEALAAGEPHPVIPGAGSRGHSAAPSLTRTDFPAPARDSL
                     PELRRYAHLMDGGRAVVAGYTETTRGCHHTCLHCPVVPLYHGRFFAIPRAVVLADIRQ
                     QVAQGAGHITFGDPDFLNGPTHALRVCRALHAEFPQLTFDITTRIEHILEHPAVITEL
                     GTLGCRFAVSAVESVSPLVLERLEKGHTKEDVVAALAVLDEAGIAMRPSLLPFTPWTT
                     LDDYLELLDFIDAHDLVAHVDPVHLSIRLLVPPSSALLEREETQRWLGPLDAANYTYT
                     WANPDPRVDALQRQIARVVEAAARDSEDVYDTFAKVRALTHQAAGLPDPGPARPHGVR
                     PPPPRLSESWFC"
     gene            complement(3340..4800)
                     /locus_tag="VFU72_08890"
     CDS             complement(3340..4800)
                     /locus_tag="VFU72_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5423642.1"
                     /translation="MPRRHFDQRIVVAIVYVCGMLMNSIDSTIVTVALATLSRQFNVS
                     PSAIDAIVVSYIVSLAVFIPASGWLGDRFGTKRTFLLALAIFTTASALCGLSGSFGQL
                     ILFRALQGMGGGMLTPVGMAMLYRTFPPEERVQVGRILMLVTILGPASGPILGGFIVE
                     KFSWHWAFFVNIPVGVSAFLFGSLFLKEHREPAAGGFDLAGFLLAGSGFASLMYALSE
                     GPNKGWTAPLVLGTGLTGALILLAFVIVELRVPAPMVQLNVLKDRLFRSCTMTSLFAS
                     AGFLGILFLVPLFLQVARGASPLSSGLTTFPEALGVVSSTQIVAWLYPRIGPRRLMCG
                     GLVWVATMMTLLCTVGLGTSDWVVRILMFMIGVGMAYVFLPSQAAAFATISRAATGRA
                     ATLSSVQRQIGAATGIALVSTVLAAVGPTRIGADGVAHAHLGAYYAAFLMSAALALIG
                     AGLALVIPDRDAAATMVMRRRGAKRDGAPVLVGEAD"
     gene            complement(5055..5474)
                     /locus_tag="VFU72_08895"
     CDS             complement(5055..5474)
                     /locus_tag="VFU72_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5423643.1"
                     /translation="MQTDNPARAHPQAHAPAHAQAAICPRFHYAVELIGRRWTGAILR
                     ALLDGATRFTDITCAVPGLSDRLLSERLRELESEAIVTRTVIPEMPVRVEYQLTEKGR
                     ALESVMAAIGAWATEWVELPEDAEACAKHGDSGDCGC"
     gene            5622..6191
                     /locus_tag="VFU72_08900"
     CDS             5622..6191
                     /locus_tag="VFU72_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5423644.1"
                     /translation="MSSSSAAQATSNTGISTWTIDPVHSSVEFSVKHMMIATVKGQFS
                     QVEGLIRFDPASHETAEVDAKIDTTSITTFNEGRDNHLRTNDFFNAEEFRYITFTSSS
                     VEKAGQDHYKVHGDLTIRDVTKPVVLDTEFDGQIVDAYGKDRAAFTAATEINRRDFGV
                     NWNAAIEGGGVVVSDKVKITLHISAVRQE"
     gene            6293..7216
                     /locus_tag="VFU72_08905"
     CDS             6293..7216
                     /locus_tag="VFU72_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423645.1"
                     /translation="MTAPNTAPTPTPTPTPTAIDPGTMVGLPALTVSDLERSLAYYTE
                     AIGFELLRRGEGEAVLGAAGQPLLELVEQPGAQPWPRGGRSYTGLYHFAILLPTRADL
                     GRWLRHWLELGLPFGQGDHLVSEALYLEDPDMHGIEVYRDRPREEWTWEDGQVRMAVD
                     PVDLRGLLAEAERAGEPWRGLPPGTRVGHIHLQVGDIRQAADFYHGILGFDIVAHMPS
                     ALFVSAGGYHHHLGLNTWHSLHAGPAPADSVRLRSFTVELPSEEARQAVVRRLDSAGI
                     PHQRLGTAVAVEDPWQNTILLRVGPTPLAEA"
BASE COUNT         1150 a         2610 c         2432 g         1062 t
ORIGIN      
        1 ccaagcgccc ggccagtacg cccaggagct gatccaggat cgcggcgtag ggcttgggga
       61 agagctgctc ggcctcgtcg gtcagttggt actcgtaggc cggcttgcgg gcgccggggc
      121 ggaagccctg ctgcaccacc aggccgtcgc gctccagcgt ggccaggtgc gagcggaccg
      181 cgttgtcggt cagctccagc tcctgggcca gttcgtccac ggtgcgcgag gagcggcgca
      241 agagggcgac gatacgcccg cgcgtcgagt tgagaaagcg atgcatccag cctggctcgt
      301 ccatgacgcc tccatcatgc tgcgtcgatt gtggcatggc gaccgccact gagaaaagtc
      361 aaacacattc ttgactttat tgccaggggg tgttatcagt ggggtaacga cgccggtgcg
      421 gtgccggcgg cgcccgagtt cgggtcctca gccgaagtag gaggtaggcg gcatgaacct
      481 cgcgaccctg atcgtctggc tggtgaccgc ggtcggcggt ctgatcctgg tcggcatctt
      541 tctctcgcgc ggcgggctcc agaccggacg cgagcaccgc ttcccgcgcc cgctcccctt
      601 cgcccacgcg ctgctggcgg tggcctcgct ggtcctcttt ctgatctggt gggccaacgg
      661 cagcgacgcg ctcaagccga tcgtgctggt cgggttgctc ttgaccgcgg tgctcggcat
      721 ctggatgttc gtcctctggc tcggcaagct gcgcgtccgc ctctcgccct cggtcggggc
      781 ggccaccacg cggcccgccg aggactcctt cccgaccgta ttggtggcgc tgcacggcat
      841 cctggcggtc tcgacgctgg tgctctacat cgtggccgcc ttcatcgtcg cgagctaagc
      901 gcgcggattt ggtagagagt agggagcgtc atggccaatc ccttacgccg tttcgccgga
      961 gaccctgtcc acccggccac cgtgcacttc ccgttggcgc tcttcccggc ggccacgctc
     1021 ttcgacgtgc tggcgctgac cctgggcgac ggcagcgtct acacccgcgg cgccttcatc
     1081 ctgatcgccg cggctagcgt ggccgccctg ctggccatga cgaccggctt cgtccagctc
     1141 ctcgacgtgc cgccggagtc acgcgcctgg cgcctggcgc tctggcacat gagcgtgcag
     1201 ttgctggcgg ccagcgtgct ggtgaccggg ttgctgctgc gactgggcca tctcgacgcc
     1261 gcgcacgcgc ccatcgcggc catcgccctc aacgccggcg gcagcgccat cctcctcttc
     1321 ggcggctggc tgggcggcca catggtcttc agccacggcg tcagcgtgga agcgggggcc
     1381 gacaccctgt tcgcggaggc gcccacgccc acgccaatcg cgcagccgct ggagctgcac
     1441 gagcgggata tcgccgcccg ccgctgatca ctcgacagca agtccttccc ccccaacccc
     1501 cacgactccc cctctcccag cactgggaga gggggctggg ggtgagggcc tgcgggcgca
     1561 ccttgctccg cgtcgccaca tcgactagcc ttcagccggg gaccggagcc gcgaagtgtg
     1621 gaggagctat cccatcgcca gccagccgtt gacccaccag acgaccgacc tgcacgcccc
     1681 cggcgcggtc ctgctcgtct cctgctacga gctgggacac cagccgctga gcctggcctt
     1741 cccgctggcg cggctgcgcc aggccggttt cgaacccggc gcggtcgata ccgcgctgga
     1801 cacgctctcc gatgaagcca tcgacgccgc gcggctggtc gccatcgcgg tgccgatgca
     1861 caccgcgctg cgactggggg ccgagatcgc cgcccgcgtg cgccggcgca acgcatccgc
     1921 cgtcatctgc tgcttcggcc tctacgcctg gctcaacgcc gagacgctgc tcgccgacac
     1981 ggccgactat gtcatcggcg gcgagagcga ggagccgctc gtggcgctgg ccgaggcgct
     2041 ggcggcgggc gagccgcacc cggtcatccc cggcgccggc agccgcggcc actcagccgc
     2101 gccgtcgctg acgcgcaccg acttcccggc cccggcgcgc gacagcctgc cggagctgcg
     2161 ccgctacgcg catctgatgg acggcggccg ggccgtcgtg gccggctaca ccgagaccac
     2221 gcgcggctgc caccacacct gcctgcactg tccggtggtg ccgctctacc acgggcgctt
     2281 cttcgccatc ccgcgcgcgg tcgtcctggc cgacatccgc cagcaggtag cccagggcgc
     2341 cgggcacatc accttcggcg atccggactt cctcaacggc ccgacgcacg ccttgcgcgt
     2401 ctgccgcgcg ctgcacgcgg agttccccca gctcaccttc gacatcacca cgcgcatcga
     2461 gcacatcctg gagcatccag cggtgataac cgagctgggt acgctcggct gccgcttcgc
     2521 cgtctcggcg gtggagtcgg ttagcccgct ggtgttggag cggctggaga aggggcacac
     2581 gaaagaggac gtggtcgcgg cgctggcggt gctggacgag gccggcatcg cgatgcgccc
     2641 ctcgctgctc cccttcacgc cctggacgac gctggacgac tacctggagc tgctcgactt
     2701 catcgacgcg cacgatctgg tcgcgcacgt cgacccggtg cacctctcga tccggctgct
     2761 ggtgccgccc agctcggcgc tgctcgagcg cgaagagacc caacgctggc tcggcccgct
     2821 ggatgccgcc aactacacct acacctgggc caaccccgac ccgcgcgtcg acgccctcca
     2881 gcgccagatc gcgcgtgtgg tcgaagccgc cgcgcgcgac agcgaggacg tctacgacac
     2941 cttcgcgaag gtccgcgccc tcacccacca ggccgccggc ctgcccgacc ccggcccggc
     3001 ccggccccac ggcgtccgcc ccccgccccc ccgcctcagc gaatcctggt tctgctgagc
     3061 agagcccacc gcggatcagt tggatccgct gcggcagtaa cgacgatcgc ccaaggttcg
     3121 cggagcgttg tggcgttccg tgtggattcg gcggcggacg gcgcgcgggg ttaaaaccac
     3181 cgcgctagat ctttacgaaa gcctgccgaa gcaggctgga gtttgagaca gtctcagcct
     3241 gcttgagcag gcttcgctta ctagcgcgtc ggcttgagcc ccgcgcgatg tcgccgtgag
     3301 gaacgcacta ggtcgtcaat ctccaccaac ctcgccccct caatccgcct cacccaccag
     3361 caccggggcg ccgtcgcgct tggcgccacg gcggcgcatg accatagtag cggcggcgtc
     3421 gcggtcgggg atgaccagcg ccaaccccgc gccgatcagg gccagggccg cggacatcag
     3481 gaaggcggcg tagtaggcgc ccagatgcgc gtgcgccact ccgtcggcac cgatgcgggt
     3541 cgggccgacc gccgccagca ccgtgctgac cagcgcgatg ccggtcgccg cgccgatctg
     3601 gcgctgcacg ctggagagcg tggccgcgcg cccggtggcg gcgcgcgaga tagtggcgaa
     3661 ggccgccgcc tgcgagggca ggaagacgta ggccataccc acgccgatca tgaacatcag
     3721 gatgcgcacg acccagtcgc tggtcccgag accgacggtg cacagcagcg tcatcatggt
     3781 cgccacccag accagcccgc cgcacatcag ccggcgtggg ccgatgcgcg ggtagagcca
     3841 ggcgacgatc tgggtcgagg agaccacgcc cagcgcctcg gggaaggtcg tcagtccgga
     3901 ggagaggggc gaggcgccgc gcgccacctg caggaagagc ggcaccagga agagaatccc
     3961 caggaagccg gccgaggcga agagcgaggt catggtgcaa ctgcggaaga gccggtcctt
     4021 cagtacgttg agctgcacca tcggcgcggg cacgcgcagc tcgacgatca cgaaggccag
     4081 caggatcagc gctccggtca ggccggtgcc cagcaccagc ggcgcggtcc agcccttgtt
     4141 gggcccctcg ctgagggcgt acatcagcga ggcgaagccg ctgccggcca agaggaagcc
     4201 ggccaggtcg aagccgccgg ccgccggctc gcggtgctcc ttgaggaaga ggctgccgaa
     4261 gaggaaggcg ctgacgccga ccgggatgtt gacgaagaag gcccagtgcc aggagaactt
     4321 ctcgacgatg aagccgccca ggatcgggcc ggaggccggg ccgaggatcg tcaccagcat
     4381 caggatgcgc ccgacctgca cccgctcctc cggcgggaag gtgcggtaga gcatcgccat
     4441 cccgaccgga gtcagcatgc cgccgcccat gccctgcagg gcgcggaaga ggatcaactg
     4501 cccaaagctg ccggagaggc cgcagagcgc cgaggcggtg gtgaagatgg ccagggccag
     4561 caggaaggtg cgcttggtgc cgaagcggtc gcccagccag ccggaggcgg ggatgaagac
     4621 cgccaggctg acgatgtagc tgaccacgat cgcgtcgatg gccgaggggg agacgttgaa
     4681 ctggcggctg agcgtggcca gcgcgaccgt gacgatggtc gagtcgatcg agttcatcag
     4741 catgccgcag acgtagacga tcgcgaccac gatgcgctgg tcgaagtgac gacggggcac
     4801 ccggttccct ccccaaagaa cagacgtgac gttctagatg cggaaggggc agacctccgt
     4861 gtctgccctc gtgcccgatt ttctcagccg cacctttacc gccagagccg ccctattccc
     4921 ctgtcgttgc cgaggcgtaa gggcgacagg gcagacacgg gggcccgccc ctaccggtat
     4981 gaccgtgaat cctaccgtgc cgttgtcttg atagcgacac cagaagcgtg tgcgtcctgc
     5041 cgccaaacgc cggatcagca gccacagtct ccggagtcgc cgtgcttagc gcacgcttcc
     5101 gcgtcctcgg gcagctcgac ccactcggtg gcccaggcgc cgatggcggc catgaccgac
     5161 tccagcgccc gccccttctc ggtgagctgg tactcgaccc gcaccggcat ctcggggatg
     5221 accgtgcgcg tgacgatcgc ctcggactcc agctcgcgca gccgctccga gagcaaccgg
     5281 tcgctcaggc cggggacggc gcaggtgatg tcggtgaagc gggtggcgcc gtcgagcagg
     5341 gcgcgcaaga tggcgccggt ccagcggcgg ccgatcagct ccacggcgta gtgaaagcgc
     5401 gggcagatgg cggcctgggc gtgcgccggg gcatgagcct ggggatgggc gcgggctgga
     5461 ttgtcggtct gcattgcggc ctccttctga cccaattcta atcttcggta agccacttga
     5521 caaccgtgag caataggtct atagtcactt accagagatt agagccgtac ccgacagaac
     5581 cgtctgatcg ggtacgaggc agaaagggca agggcagaga catgtccagc agcagcgccg
     5641 cacaggcgac cagcaatacc ggtatcagca cctggacgat cgatccggtc cactccagcg
     5701 tcgagttcag cgtcaagcac atgatgatcg cgacggtcaa gggtcagttc tcgcaggttg
     5761 aggggctgat ccgcttcgac ccggccagcc acgagacggc cgaggtcgac gccaagatcg
     5821 acaccaccag catcaccacc ttcaacgagg ggcgcgacaa ccacctgcgc accaacgact
     5881 tcttcaacgc cgaggagttc cgctacatca ccttcaccag cagcagcgtg gagaaggccg
     5941 gccaggacca ctacaaggtc cacggcgatc tcaccatccg cgacgtcacc aagccggtcg
     6001 tgctcgacac cgagttcgat ggccagatcg tcgacgccta cggcaaggac cgcgccgctt
     6061 tcaccgccgc gaccgagatc aaccggcgcg acttcggcgt caactggaac gccgcgatcg
     6121 agggcggcgg cgtggtcgtc agcgacaagg tcaagatcac gctgcatatc tcggcggtgc
     6181 gccaggagta acccagcacc ggcgagcgtg gcacgctgcg cgctgtagct gtgccacgct
     6241 cggaccgccg ggcggcccgc cggccgtcgt gtctcaacca aggagatttt ccatgaccgc
     6301 acccaacacc gctcccacac ccacacccac ccccaccccc accgccattg atccggggac
     6361 gatggtcggg ttgcctgctt tgaccgtctc cgatctggag cgttcgctgg cctactacac
     6421 cgaggcgatc ggcttcgagc tgctgcgccg gggcgagggc gaggccgtcc tcggcgcggc
     6481 cgggcagccg ttgctcgagc tcgtcgagca gccgggcgcc cagccctggc cgcgcggcgg
     6541 gcgcagctat accggcctct accacttcgc catcctgctg ccgacgcgcg ccgacctcgg
     6601 ccgctggctg cgccactggc tggagctggg cctgcccttc ggtcagggcg atcacctggt
     6661 cagcgaggcg ctctacctgg aagaccccga catgcacggg atcgaggtct accgcgaccg
     6721 cccgcgcgag gagtggacct gggaggatgg gcaggtgcgg atggcggtcg acccggtcga
     6781 tctccgtggt ctgctggccg aggccgagcg cgccggagag ccctggcgcg ggctgccgcc
     6841 gggcacgcgc gtcggtcaca tccacctgca ggtgggcgac atccgccagg cggccgactt
     6901 ctaccacggc atcctcggct tcgacatcgt ggcccacatg ccgagcgcgc tcttcgtctc
     6961 ggccggcggc taccaccacc acctcggcct gaacacctgg cacagcctgc acgccggccc
     7021 ggcgccggcc gactccgtgc gcctgcgctc cttcacggtc gagctgccca gcgaggaagc
     7081 gcgccaggcg gtcgtgcggc ggctcgatag cgccggcatc ccccaccagc gcctcggcac
     7141 ggccgtcgcc gtcgaggacc cctggcagaa caccatcctg ctgcgcgtcg gcccgacgcc
     7201 gctggcggag gcgtgacggg ccctcacccc tgacccctct cccgtgcgcg ggag
//
LOCUS       DASUBI010000464         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_136387, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000464 DASUBI010000000
VERSION     DASUBI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136387"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1227)
                     /locus_tag="VFU72_08910"
     CDS             complement(277..1227)
                     /locus_tag="VFU72_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423646.1"
                     /translation="MAEDVKSETALRSAAVSSSAARLSRAEQTVPRTYLQQSWTRFKH
                     NRLAVGSLVVVILILIFGFGAPLISHFITHQGYADQSLLDRFKKVGSSGHILGTDNLG
                     RDVFTRLAYAVRVSMEVALLGTVSALTLGVALGSIAGYYGGFVDTVIMRLVDTLLSIP
                     TLYLLIFVGAMFTVGPSVLALLIAAVGWMGLSRLVRGEILAVRNREYVIAAQVVGVPD
                     RRIITRHIIPNIAPIVIVWATLQLPAFILIEAALSFLGLGVHPPVPSLGNMLNGAITY
                     MSQSWTLVFIPGFVIYLAVLAINLFGNGLRDALDPRLGER"
     gene            complement(1243..2193)
                     /locus_tag="VFU72_08915"
     CDS             complement(1243..2193)
                     /locus_tag="VFU72_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423647.1"
                     /translation="MGRYLIRRLIQLIPLVIGITIITFGLANLVPGSPVSDLEFNPDI
                     RPVDKQRIIHNLGLDQPVYIRYIQWISNVARGDLGISLVTYKPVTQSILQKLPNTLLL
                     TVSALLFALIVSIPVGTYAAVKRNSWFDQITTVGSVAGFAVPSFWLGLMMILVFSVKF
                     HLLPSGGVYTLGHATLADRAKHLIMPAFVLGFVQMAAWTRYIRSQMIEVLRQDYMRTA
                     EAKGLVQAKVIFRHGLRNAILPLVTLIGLDLPALFGGSVIIEQIFTWNGLGRFILDAV
                     SKRDYTVVMGITLFISTLTVVANLLADLAYGLLDPRITYS"
     gene            complement(2355..3863)
                     /locus_tag="VFU72_08920"
     CDS             complement(2355..3863)
                     /locus_tag="VFU72_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423648.1"
                     /translation="MVEGTFADAKTLNPVLESDTSSGEVISLIFNGLVLVDPSTVQPA
                     ADLATKWDISSDGLTYTFTLGDGIKFQDGQALSSDDVKFTYDLLMNKATNSPRTSELT
                     DRVKSVEAKDPKTVVFTLNHPTAPFLVSNAGYGILPQHALKDVDPAGLAQNDFSTGKK
                     GVTIGTGPFMFEEWVKDDHMTLVKYDGYFKGAPNLDKYLYKVVPNQTVISAQLKTGEI
                     DYSTIQPADLQGMQSETSVAVVPYDTFSFTFYAYQLDASKSDLFQDKAVRQALLYALD
                     RKAMVDAIYFGQAAVAVGTEPVLSWAYAPDQIKLKYDFDVNKANQLLDQAGWVKGSDG
                     IRAKNGKKLSFSVWTNAGNAVREQFVTVFQQQWKKIGIDAKPQTQEWNAFLDRITSSH
                     DFEIFLVGFQWDVDPDQSTMWSSDAYNGGFNMNKYNNAEVDKILKDALETSDINKRKQ
                     LYIQMQNIVMDDLPSAILVFPKALAVVNKRVHNLFPNAVSVTFNAHQWWVNG"
     gene            3910..>4131
                     /locus_tag="VFU72_08925"
     CDS             3910..>4131
                     /locus_tag="VFU72_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423649.1"
                     /translation="MGAGVPLAVAEVAAGVAPVVVVAAAAVLAGEAAPAPVLVVGAEV
                     AAELVPAGVLLLLEPPQAATSPSAAAASAD"
BASE COUNT          752 a         1258 c         1375 g          746 t
ORIGIN      
        1 cgtgagcgcg catcgcgggg gtgacacggc gcatgggtgg tgatcgacgg tgctgcgtgc
       61 cgctccacga cggatccgtc gactgcgctc aggatgacgg ggggaggggg tggcgtacga
      121 gcacagccgg tcgtagtcgt cagcctctca tgtaccctgt catcgcgagc gcagtcgagg
      181 tatccgtgcg aacgcggcac gcgacgaaaa gggcgggttc aacacccgcc cctctggatc
      241 cttcgttctg ccctctctcg gcctggagcc ccagtcctag cgctcgccca ggcgcgggtc
      301 gagcgcgtcg cgcaggccgt tgccgaagag gttgatcgcc agcaccgcga gatagatcac
      361 gaagcccggg atgaagacca gcgtccacga ctgcgacatg taggtgatcg cgccgttgag
      421 catgttcccc aggctgggca ccggcggatg cacgcccagt ccgaggaagc tcagcgccgc
      481 ctcgatcagg atgaaggccg gcaactgcag cgtggcccag acgatcacga tcggcgcgat
      541 gttggggatg atgtgccgcg tgatgatgcg ccgatccggc acgccgacca cctgcgccgc
      601 gatcacgtac tcgcggttgc gcaccgccag gatctcgccg cgaacgaggc gcgacaggcc
      661 catccagccg accgccgcga tcagcagcgc cagcacgctc gggccgacgg tgaacatggc
      721 gccgacgaag atcagcaggt agagggtcgg gatcgagagg agggtgtcga ccagacgcat
      781 gatgaccgtg tcgacgaagc cgccgtagta gccggcgatc gagccgagcg cgacgccgag
      841 ggtcaaggcg ctgaccgtac cgagcagcgc cacctccatc gagacacgta ccgcgtaggc
      901 cagccgggtg aagacgtcac ggcccagatt gtcggtgccc aggatatgcc ccgatgagcc
      961 caccttcttg aagcggtcca gcagcgactg atccgcgtac ccctggtgcg tgatgaagtg
     1021 cgagatcaag ggcgccccga acccaaagat cagaatcaga atcacgacca ccagcgaccc
     1081 gaccgccagg cggttgtgct tgaagcgggt ccatgactgc tgcagatagg tacgcgggac
     1141 ggtctgctcc gcgcgcgaga gcctcgccgc gctgctcgat acggcggcgc tccgaagcgc
     1201 ggtctcgctc ttgacgtcct cggccacgat ccccgcctcc ctctacgaat acgtaatccg
     1261 cggatcgagc agaccgtacg ccagatccgc cagcagattc gcgacgaccg tgagcgtcga
     1321 gatgaagagc gtgatgccca tcaccaccgt gtaatcgcgt ttggataccg cgtcgagaat
     1381 gaagcgcccc agtccgttcc aggtgaagat ctgctcaatg atcaccgagc cgccgaagag
     1441 cgccggcaga tcgagtccga tcagggttac cagcggcaga atcgcgttgc gcaaaccatg
     1501 gcggaagatg acctttgcct gcaccagccc ctttgcctcc gcggtgcgca tgtagtcctg
     1561 ccgcagcacc tcgatcatct gcgagcgaat gtagcgcgtc caggcggcca tctgcacgaa
     1621 accgagcacg aaggctggca tgatcaggtg tttggcccga tcggccagcg tggcgtgccc
     1681 gagggtgtag acacctccgg acggcaataa atggaatttt actgagaaga ccaaaatcat
     1741 catcaagccg agccagaaag agggcacggc aaagccggcc accgatccaa ccgtggttat
     1801 ttgatcgaac caggagttgc gtttgaccgc cgcataggtg ccgaccggga tggagacgat
     1861 cagcgcgaag aggagcgcgc tgacggtaag caggagcgtg ttggggagct tctgcaagat
     1921 cgactgagtc accggcttgt aggtcaccag cgagatgccg agatcgcccc gcgccacgtt
     1981 cgagatccat tggatgtagc ggatgtagac cggctggtcg agacccagat tgtggatgat
     2041 gcgctgcttg tcgacggggc gaatgtcggg gttgaactcg aggtcggaga cggggctgcc
     2101 ggggacgaga ttggcgaggc cgaaggtgat gatcgtgatg ccgatgacca acgggatgag
     2161 ttggatcaag cggcgaatca ggtagcggcc catgaacgta tcctcgcctt ctccctcagc
     2221 agcgaaaaag tccgtctggg tgtgagttta catgattaat caccgcaggg aagacccctg
     2281 cggtcaattg gccggccgct cgtgatccga ctagcggcgg cccaaatggg ccgccgccgt
     2341 cgctgtcgct cgcgctagcc gttcacccac cactgatggg cattgaaggt gacgctcacg
     2401 gcgttcggga agaggttgtg cacgcgctta ttgaccaccg ccagcgcctt ggggaagacg
     2461 aggatcgcgc tcggcaggtc gtccatcacg atgttttgca tctggatgta gagctgcttg
     2521 cgcttgttga tgtcgctggt ctcgagcgcg tccttcagga tcttgtccac ctccgcgttg
     2581 ttgtacttgt tcatgttgaa cccgccgttg taggcgtcgc tcgaccacat cgtgctctga
     2641 tccgggtcga cgtcccactg gaagccgacc aggaagatct cgaagtcgtg cgaactggtg
     2701 atgcggtcga ggaaggcgtt ccactcctgc gtctgcggct tggcgtcgat gccgatcttc
     2761 ttccactgct gctggaagac ggtgacgaac tgctcgcgca ccgcgttgcc ggcattggtc
     2821 cagacgctga aggagagctt cttgccgttc ttggcgcgaa tcccatccga gcctttaacc
     2881 cagcccgcct gatccagcaa ttggttcgcc ttgttcacgt cgaagtcgta cttcagcttg
     2941 atctgatcgg gcgcgtaggc ccaggagagc accggctcgg tgcccaccgc cacggccgcc
     3001 tggccgaagt agatcgcgtc caccatggcc ttgcggtcga gcgcgtagag gagcgcctgg
     3061 cgcaccgcct tatcctggaa cagatcgctc ttggaggcgt cgagctggta ggcgtagaag
     3121 gtgaagctga acgtgtcgta cgggacgacg gcgacgctcg tctcgctctg cattccctgc
     3181 aggtcagccg gctgaatggt gctgtagtcg atctcgccgg tcttgagctg cgcgctgatg
     3241 acggtctggt tcggcaccac cttgtacagg tatttgtcga gattcggcgc gcccttgaag
     3301 tacccgtcgt acttcaccag cgtcatatgg tcgtccttga cccactcctc gaacatgaag
     3361 gggccggtgc cgatcgtcac gcccttcttg ccggtcgaga agtcattctg ggcaagcccg
     3421 gccgggtcga cgtccttcaa ggcgtgctgc ggcaggatac cgtagccggc gttggagacc
     3481 aggaacggcg cggtcgggtg attgagggtg aacacgacgg tcttgggatc cttggcctcg
     3541 accgacttga cgcggtcggt cagctcagag gtacgcggcg aattggtcgc cttgttcatc
     3601 agcaggtcat aggtgaactt tacgtcgtcg ctagagagcg cctggccatc ctggaacttg
     3661 atgccgtcgc cgagggtgaa ggtgtaggtc aggccgtcgc tggagatatc ccacttggtg
     3721 gccaggtcgg cggccggctg caccgtcgag gggtcgacca gcaccagccc attgaagatc
     3781 agcgagatca cctcgccgga ggaggtgtcg ctttcgagca ccgggttgag cgtcttcgcg
     3841 tcggcgaagg tgccttcgac catctggccg cccttgtgct gcgccggcac cacattgaac
     3901 ttctgcccgg tgggcgccgg cgttccactg gccgtcgcgg aggtcgcagc cggtgtcgcg
     3961 ccggtcgtgg tggtggcggc cgccgctgta ctggccggcg aggccgctcc ggcgccggtg
     4021 ctggtggtgg gagcggaggt cgcggccgag ctggtgccag ccggggtatt gctgctgctg
     4081 gagccgccgc aggcagcgac cagccccagc gcggccgccg ccagcgccga t
//
LOCUS       DASUBI010000465         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_674840, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000465 DASUBI010000000
VERSION     DASUBI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674840"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2136
                     /locus_tag="VFU72_08930"
     CDS             <1..2136
                     /locus_tag="VFU72_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5423650.1"
                     /translation="QRPLPLLVTQVIEGRLARLDEKTRGLLQVAAIVGQDVPLELWQR
                     VSAAPEEALAAAIKQGQDSQLIEEAGGGERYHFRHALLRLALYEEVVAPSRRPWHRRV
                     AEALAETPNPDPDQVAHHLQQAGDARAIEWLIKAGERAQLTYAWEIAAQRFDAALSRL
                     TEQGAPAAERAVLLRRMAELVRYMDSRRALALVDEAQRLAQEAGEPGLALTSQVDSGL
                     YLCFLGDYRAGLDRMVEAVDAFAVLPTSSQARAWELVGSTDVLAGTLVQWLTVVGRLE
                     EAHARGLRLLDEAPAPDFEPKQPGSSFADGLFGLARAQALLGEPANARRTSRQARAIY
                     HAVGHHLMEVVSSSSELEHVQLPYFPEQLEERRLLAEAISAAARRAVQNQPAARFMCD
                     LLPLQMLEGNWVAAREALQGLASGLGVFDSEAAGYAALLAHHQGEPGTARELIAGLLP
                     QGPRAEPGNVPLWAGLTAQLLAAELALDASELALARAWLEAHDRWLGWSAAVLGRSEG
                     ALAWAAYHRASGDLPLARRLAAQALEHASDPRQPLAQLAAQRFLGQLHSEARQFGAAE
                     SHLEQALALAAACAAPFERALTLLELARLRTAQGRPDDARVLLAEVRTLCLPLDARPT
                     LQRVDELERQLDDAAARRGRATYPAGLSAREVAVLRLVAQGLTDAQVAEQLFISRRTV
                     SSHLLSIYNKLGVPSRAAATRFAVEHQLL"
     gene            2397..2687
                     /locus_tag="VFU72_08935"
     CDS             2397..2687
                     /locus_tag="VFU72_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423651.1"
                     /translation="MSALALLLALPAGVALQRALSSGTPARSAAATIKAPAAPAAMPH
                     WQFVEMNQMPESSAVSDASAPTTGVPHWKFVEMNQLPETASDEPIVQPNGRR"
     gene            2849..3913
                     /gene="add"
                     /locus_tag="VFU72_08940"
     CDS             2849..3913
                     /gene="add"
                     /locus_tag="VFU72_08940"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5423652.1"
                     /translation="MSLESYLRAAPKAELHVHLEGAIQPATLLELARRNGVDLPANSE
                     AELRDWFTFRDFDHFVEIYVSITRCLRTVEDYRQIVRDFGAEMARQNVRYAEVTFSPS
                     THHALGVPQDVWFSGLSQGRDEIKRDFGVEVNWVFDIVRNSILHEGITSRADYTTGVA
                     IEGQGEGVVALGLGGAEAGAPPEPYAAWFERARAAGLHSNPHAGELAGPESIWGALSV
                     LGAERIGHGVRAIDDPALVRYLAEQRIPIEVSPTSNLRLGVYPDYARHPLRALHEAGV
                     IVTVNSDDPPLFNTTLNDEVLLMPNVFGFDLAAIDELLLNPVRHSFLPPERKAALEAE
                     FRAELAALKAEHLGTSVADE"
     gene            complement(4130..>5055)
                     /gene="aspS"
                     /locus_tag="VFU72_08945"
     CDS             complement(4130..>5055)
                     /gene="aspS"
                     /locus_tag="VFU72_08945"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEU5423653.1"
                     /translation="PRQRALFRLSAASMSGFRSTLRALDFVEIQTPKIVASATESGAN
                     VFAIDYFGRPAYLAQSPQLYKQIMVGVFERVFEVGPVFRAEPHDTPRHLNEYVSLDAE
                     MGFIEDHTTVMRVLTQVLAGMVRAVGQEEAALATLGVALPVVPAEIPAIHFAEALELI
                     AAATGEDVRGEPDLAPAHERWLGEWARREHGSEFLFVVGYPAAKRPFYTHPDPARPGY
                     TNGFDLLFRGLELVTGGQRLHRYEDYLAALATRGQDPEPLAGYLEAFKHGMPPHGGFA
                     LGLERWVARLTGAANIRETTLFPRDLNRLTP"
BASE COUNT          766 a         1753 c         1784 g          752 t
ORIGIN      
        1 cagcgcccgc tgccgctgct cgtgacacaa gtgatcgagg ggcgcctggc gcggctggac
       61 gagaagactc gcgggctgct tcaggtggcg gcgatcgtgg gccaggacgt gccgcttgag
      121 ttgtggcagc gcgtgagcgc ggcgccagaa gaggcgctgg cggccgccat caagcagggg
      181 caggactctc aactaatcga agaggccgga ggcggcgagc ggtatcactt ccggcatgcg
      241 ctcttgcgcc tcgcgctcta cgaagaggtg gtcgcgccca gtcgcaggcc gtggcatcga
      301 cgagtggccg aagcgctggc ggagacgccg aatccggatc ccgaccaggt cgcgcatcat
      361 ctccagcaag ccggcgatgc gcgagcgatc gagtggttga tcaaagccgg cgagcgcgcc
      421 cagctgacgt atgcgtggga gatcgcggcc cagcgcttcg acgccgccct gagccggctc
      481 accgagcaag gcgcgcccgc ggcggagcgg gcggtgttgc tcaggcgcat ggcggagttg
      541 gtccgctaca tggattcgcg ccgggcattg gcgctggtgg atgaggcgca gcggctggcg
      601 caggaggcgg gcgagcctgg gctggcgctt acgagccagg tcgattcggg gctctatctg
      661 tgctttctcg gagattaccg cgccggtttg gaccgcatgg tcgaggcggt tgacgcgttc
      721 gccgtgctgc cgaccagctc ccaggcgcgc gcctgggagc tggtcggcag cacggatgtg
      781 ctggcgggta cgctggtcca atggctcacc gtcgtcggac gactggagga agcgcacgcg
      841 cgtggcctgc ggctgctgga tgaggcgcca gcgccggact ttgagccgaa acagcccggc
      901 tccagcttcg ccgatgggtt gttcgggctg gcacgcgccc aggcactgct gggtgagccg
      961 gcgaacgccc gacggacgag ccggcaagcc cgcgctatct atcatgcggt cggccaccat
     1021 ctcatggagg tcgtgtcatc ctccagtgag ctggagcacg tgcagcttcc ctatttccca
     1081 gagcagctcg aggagcgccg gctcctggca gaggccattt cggccgcagc gcgacgcgcc
     1141 gtgcagaacc aacccgccgc gcgtttcatg tgtgatctcc tgcccttgca aatgctcgaa
     1201 ggcaactggg tggcagcgcg ggaggcgcta cagggtctgg cctcgggcct cggcgtgttc
     1261 gactcggagg ctgccggcta cgccgcgctc ctggcgcacc accagggcga gccgggcacg
     1321 gcgcgtgagc tgatcgccgg gctgttgccg caggggcccc gtgcggagcc cggcaacgtc
     1381 ccgctgtggg ccgggctcac cgctcagttg ctggccgccg aacttgcgct cgatgcgtcc
     1441 gagctcgcgc tggcccgagc ctggctggag gcgcacgacc gttggcttgg ctggagcgcc
     1501 gcggtactgg gtcggtccga aggggcgctg gcctgggcgg cgtatcaccg cgcgagcggc
     1561 gatctgcccc tcgcccgccg gctggccgcg caggcactgg aacacgcctc cgacccccgc
     1621 cagccgctgg cgcagctcgc cgcgcagcgc ttcctcggcc agctgcactc cgaggcacgc
     1681 caattcggcg cggccgagtc gcacctggag caggccctcg cgctggccgc ggcctgtgcc
     1741 gcgccgtttg agcgcgcgct gacgctgctt gagctggcgc ggctgcgcac ggcgcagggg
     1801 cggcccgacg acgcgcgagt gctgctggcc gaggtccgca ccctgtgcct gccgctggac
     1861 gccaggccga cgctgcagcg cgtcgacgag ctggagcggc agctcgatga cgccgccgcc
     1921 cgccgggggc gtgccaccta cccggcgggc ctgtcggcgc gcgaggtggc ggtgctgcgc
     1981 ctggtggccc agggcctgac cgacgcccag gtcgccgagc agctcttcat cagccgccgc
     2041 accgtcagca gccacctcct ctccatctac aacaagctcg gcgtcccctc gcgcgccgcc
     2101 gcgacccgct tcgccgtcga gcaccagctc ctctgagccg tactgaccgc ccgctcggcg
     2161 cccgtcccga gagacagtaa ttctacgtac cggtccctca accacaaacg ctcgggaatc
     2221 cggtagtccg ccggatgagc ccgcgccact ggcagcctag attggtctca gtcggagacg
     2281 cggtgagatc aagaaaggaa acggcagtgg ttggacaacc atcttcccga acagcgcgaa
     2341 atcggttcgg ttccgcggcg aatgtcctcc atgaccgcac gcaacgaatc gtggtcgtga
     2401 gcgcgctggc gctgctgctg gccctgccgg ccggggtcgc cctgcagcgg gcgctctcgt
     2461 cagggacgcc ggcgcgatcg gccgcggcca cgatcaaggc acccgcggcg cccgcggcga
     2521 tgccgcattg gcagttcgtc gagatgaatc agatgcccga gtcgtccgcc gtgagcgacg
     2581 cttctgcccc gacgacgggc gtcccgcact ggaagttcgt cgagatgaac caactgccgg
     2641 agacggcgag cgacgaaccg atcgtgcagc cgaacgggcg ccggtagcag gcgacgcgcg
     2701 tcctgaacgc gggcagaggc ggctcggagt ctccccgagc cgcctcttgg ttttccggta
     2761 gggcactcac ggctcgccgt ggcgacgcgt gctaccattc gccgaaccgc caaggtggcg
     2821 ccgctacgat tgccgtgagg agcgatgaat gtcgttggag agctacctgc gcgccgcgcc
     2881 gaaggcggag ttgcacgtcc acctggaggg cgccatccaa ccggcaacgc tgctggagct
     2941 ggcgcggcgc aacggcgtag atctgccagc caacagtgag gcggagctgc gcgactggtt
     3001 caccttccgc gacttcgacc acttcgtcga gatctacgtc agcatcactc gctgcctacg
     3061 cacggtcgag gattaccgcc agatcgtgcg cgacttcggc gccgagatgg cgcgacaaaa
     3121 cgtgcgctac gccgaggtca ccttctcccc cagcacccac cacgcgctgg gcgtaccgca
     3181 ggatgtctgg ttcagtggcc tgagccaggg gcgggacgag atcaagcgcg acttcggggt
     3241 cgaggtcaac tgggtcttcg acatcgtgcg caacagcatt ctccacgagg gcatcaccag
     3301 ccgcgccgac tacacgaccg gcgtggctat cgagggacag ggcgagggcg tggtggcgct
     3361 tggtctgggc ggagccgagg cgggcgcgcc gcccgagccc tacgcggcct ggttcgagcg
     3421 cgcccgcgcc gccgggctgc acagcaaccc gcacgcgggc gagctggccg ggccggagag
     3481 catctggggc gcgctcagcg tcctcggcgc cgagcgcatc ggccatggcg tgcgcgccat
     3541 cgacgacccc gcgctggtgc gttatctcgc cgagcagcgc atcccgatcg aagtcagccc
     3601 cacctcgaac ctgcgcctcg gcgtctaccc cgactacgcc cgccacccgc tgcgcgccct
     3661 gcacgaggcc ggcgtgatcg tcacggtcaa ctccgacgat ccgcccctct tcaacaccac
     3721 cctgaacgac gaggtgctgc tcatgcccaa cgtcttcggc ttcgacctgg ccgcgatcga
     3781 cgagctcttg ctcaacccgg tgcgccacag cttcctgccg cccgagcgca aagccgcctt
     3841 ggaggccgag ttccgcgccg agctggccgc attgaaggcg gagcacctgg gcacgtcggt
     3901 ggcagacgaa tagatggcgc gatagggaga gacggatccc tccgactgct ctcgggatga
     3961 cagggtgaga ttggggcgga cgacgacggt ttgcctcgtc cgccaggccc ctccctctcc
     4021 cggtcatacc gagcgcagtc gagggatccg tcctcacccg gcacgatttc tcctcgacgg
     4081 gcagtgcccc tctcccacgt ctgtgggaga ggggtcaggg gtgagagcgc tacggcgtca
     4141 gccggttcaa atctcttggg aagagcgtcg tctcgcggat gttggccgcg ccggtcaggc
     4201 gggcgaccca gcgctccagt cccagcgcga agccaccgtg gggcggcatg ccgtgcttga
     4261 aggcctccag gtagcccgct agcggctcgg ggtcctggcc gcgcgtcgct agagccgcca
     4321 gatagtcctc gtagcggtgc agccgctgcc cgccggtcac cagctccagg ccacgaaaga
     4381 gcaggtcgaa gccgttggtg tagccgggcc gcgccggatc ggggtgggtg tagaaggggc
     4441 gcttggcggc cgggtaaccg accacgaaga gaaactcgga gccatgctcg cgccgcgccc
     4501 actcgccgag ccagcgctcg tgtgccggcg ccaggtccgg ttcgccgcgc acatcctcgc
     4561 cggtcgccgc cgcgatcagc tccagcgcct ccgcgaagtg gatcgccggg atctcggccg
     4621 gcaccaccgg cagcgcgacg ccgagcgtgg ccagcgcggc ctcctcctgt ccaactgccc
     4681 ggaccatccc ggccagcacc tgcgtcagca cgcgcatgac cgtcgtgtgg tcctcgatga
     4741 agcccatctc ggcgtcgagc gagacgtact cgttgaggtg acgcggcgtg tcgtgcggct
     4801 cggcgcggaa gacgggaccg acctcgaaga cgcgctcgaa cacgccgacc atgatctgct
     4861 tgtacagttg cgggctttgc gccaggtagg cggggcggcc gaagtagtcg atggcgaaga
     4921 cgttggcccc gctctcggtc gccgaggcca cgatcttggg cgtctggatc tccacgaagt
     4981 ccagcgcgcg cagggtggac cggaaccctg acatgctcgc cgcggacagg cgaaacaggg
     5041 cgcgctggcg cgggt
//
LOCUS       DASUBI010000466         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_679381, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000466 DASUBI010000000
VERSION     DASUBI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679381"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1061)
                     /locus_tag="VFU72_08950"
     CDS             complement(288..1061)
                     /locus_tag="VFU72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5423654.1"
                     /translation="MGRCNHYRIGEFAKLTGVSVRTLHHYDRIGLLHPSVRSRAGYRL
                     YTGAELLRLQQVLTLRALGFRLTEIEALLAQPDFNLIASMRVQRDILREQIAELERVE
                     AALDTLLRERQARGDWDWQLLAETSAALERRLRRRGVDVDEYYKPEEMQRRMRELAEE
                     NDITPEEIHQVEEGWTALLAEIRASYHLDPASSAARALHDRWQALLEQTMRGFRQDEK
                     LVNTIAEGYRQGRYAHIDCAPRPEDFAFIERVAKARDSA"
     gene            1164..>2597
                     /locus_tag="VFU72_08955"
     CDS             1164..>2597
                     /locus_tag="VFU72_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEU5423655.1"
                     /translation="MGESPPVRRRSYRQLRRYRQIADVLARHGLGFLIDLFGLERFLP
                     QYRGFLGRQPRARAYTRPEHLRLALEELGPTFIKLGQILSTRPDLLPPAYQAELAKLQ
                     DAAAPIPFAAVRETVESELGRPIEELFASFEETPLAAASIGQAHAATLHGGVDVVVKV
                     RRPAAVEQVEVDLAILLNLATVASRRSQLAEQYDLVALVEEFAATLRAELDYEREAHN
                     AERIAASFADEPGLQVPRVYHQLSTQRVLTLERIRGIKISNLAALEAAGIDRAALAND
                     AARMVLKMALEDGFFHADLHPGNLFVEPSGRIGLIDFGMVGTLDEATRDHLVDLLIAI
                     VGQDTDRMVDELLELGAARRIDRAVLRRDLQRIVSHYINRPLGEIEIGAVIEDALGIV
                     RRHRLQLPANLALLLRMLVMAEGLGQLLDPAFEALPVARPFGRRLLLQRYSPARWARR
                     MGQAAFDAAELGTELPRQVRRLIHEIER"
BASE COUNT          374 a          905 c          895 g          423 t
ORIGIN      
        1 ccattcgagg tgtccgccgg agagctcgcg gacgcgcttg atgatggcgt agccgtagct
       61 gttgccctgg gcgagaatcg ccaggacgat gggcgtcgag gacgcggcga tcagatcctt
      121 gttgatctcc atcattccac ccgtcgcacc gtgccaatac ctgtagtggg gtctacagag
      181 cggcattata catggcagcg ctaggtatgt gaagggcgac gcgggcagcc agggcgtcta
      241 tagccctggc tgcccgcgag gtgcgcggag ttcggctgcg agatgcgcta cgcggaatcg
      301 cgcgccttgg ccacgcgctc gatgaaggcg aagtcctcgg ggcgcggggc gcagtcgatg
      361 tgcgcgtagc ggccctggcg gtagccctcg gcgatggtgt tgaccagctt ctcgtcctgg
      421 cggaagccgc gcatcgtctg ctccagcagc gcctgccagc gatcgtggag cgcccgcgcc
      481 gcggagctgg ccgggtcgag gtggtagctg gcgcggatct cggcgaggag cgcggtccag
      541 ccctcctcga cctggtggat ctcctcgggg gtaatgtcgt tctcctcggc cagctcccgc
      601 atgcggcgct gcatctcctc cggcttgtag tactcgtcca catcgactcc tctccgtcgt
      661 agtcttcgct ccagcgcggc cgaggtctcg gccagcagtt gccaatccca gtcgccgcgc
      721 gcctgccgtt cgcgcagcag cgtgtccaga gcggcctcta cccgctccaa ctccgcgatc
      781 tgctcgcgca ggatgtcgcg ctgcacgcgc atcgaggcga tgaggttgaa atcgggctgt
      841 gcgagcagcg cttcgatctc ggtcagccgg aagccgagcg cgcgcagcgt cagcacctgc
      901 tgcaggcgca gcagctccgc cccggtgtac agccggtaac ccgcccggct gcggaccgag
      961 gggtgcagca ggccgatgcg gtcgtagtgg tgcagcgtgc gcaccgagac gccggtcagt
     1021 ttggcgaact ccccgatgcg gtagtgattg cacctaccca cgcgccacct cctctcccca
     1081 cgaggataga gcctgacgtt gcgtcagggt caagggccac gagctgccgg ccacgatctt
     1141 ctggtgtgcg gtagcatctc gccatgggag agtcgccgcc agtccgccgc cggagctacc
     1201 gccaactgcg ccgctatcgc cagatcgcgg atgtgctggc ccgccacggc ctgggctttc
     1261 tgatcgatct cttcgggctg gagcgatttc tcccccagta ccgcggcttc ctgggacgtc
     1321 agccacgtgc ccgcgcctac acccgccccg agcacctgcg cctggcgctg gaggagctgg
     1381 gcccgacctt catcaagctg ggccagatcc tctccacccg ccccgatctg ctgccgcccg
     1441 cctatcaggc ggagctggcc aagctgcagg acgccgccgc cccgatcccc ttcgccgccg
     1501 tgcgcgagac ggtggaaagc gagctgggcc gccccatcga ggaactcttc gccagcttcg
     1561 aggagacgcc gctggcggcg gcctcgatcg gccaggccca cgccgccacg ctgcacggcg
     1621 gcgtggacgt cgtggtcaag gtgcggcgcc cggcggcggt cgagcaggtc gaggtcgatc
     1681 tggcgatcct cttgaacctg gcgaccgtcg ccagccgccg ctcgcagctg gccgagcagt
     1741 acgacctggt ggcgctggtg gaggagttcg ccgccaccct gcgcgccgag ctggactacg
     1801 agcgcgaggc gcacaacgcc gagcgcatcg ccgccagctt cgccgacgag ccgggcttgc
     1861 aggtgccgcg cgtctaccac cagctgagca ctcagcgtgt gctgactcta gagcgtatcc
     1921 gcggcatcaa gatcagcaat ctggcggcgc tggaggcggc cggaatcgat cgcgccgcgc
     1981 tggcgaacga cgccgcgcgc atggtgctca agatggcgct ggaggacggc ttcttccacg
     2041 ccgatctgca ccccggcaat ctcttcgtcg agccgagtgg acgcatcggc ctgatcgact
     2101 tcggcatggt cggcacgctg gatgaggcca cgcgcgacca cttggtcgac ctcttgatcg
     2161 cgatcgtcgg ccaggatacc gaccgcatgg tcgacgagct gttggagctg ggcgcggccc
     2221 ggcgcatcga ccgcgcggtg ctgcgccgcg acctgcagcg catcgtgtcg cactatatca
     2281 accgcccact gggcgagatc gagatcggcg ccgtaatcga ggacgcgctg gggatcgtcc
     2341 ggcggcaccg gctgcagttg ccggccaacc tggcgctgct gctgcgcatg ctggtgatgg
     2401 cggaggggct gggccagctg ctcgaccccg cgttcgaggc gctgccggtg gcgcgcccct
     2461 tcggcaggcg gctgctccta cagcgctact cgcccgcccg ctgggcacgg cgcatggggc
     2521 aggcggcctt cgacgccgcc gagctgggca ccgagctgcc gcgccaggtg cgccggctga
     2581 tccacgagat cgagcgc
//
LOCUS       DASUBI010000467         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_639500, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000467 DASUBI010000000
VERSION     DASUBI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639500"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1127
                     /locus_tag="VFU72_08960"
     CDS             69..1127
                     /locus_tag="VFU72_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5423656.1"
                     /translation="MSGATKARQGEQSGTGASGWRLALQIGLQSLKDLLVDNGPQWAA
                     AIAYYTLLSVFPLLLLAAAIAAFFVDPQWAVDRATHLLGDYLPQGHDTVRQVVAGAVE
                     HRGQIGFLSALGLLWSGTRVFAALTKALNVANGSSAQYGFLARLVVEAAMLLTIGVVL
                     TLALMSGSLFSLLWPLLEHAPGSPGLVITLVSWAMRALLLIGAYFLVYQFVPQGKQQP
                     RASLTGALVATALFLSADPIFRHYVGNSGRYNLVYGSLAIIIIALLWVWIAALITLFG
                     GEVAAHTRAIAIERRSPAEVGRRHVARAQPPRPRGAATVPGAARQGAAQAGNLLDRLG
                     DRIQRLVDRVSGQQRGRV"
     gene            complement(1332..1688)
                     /locus_tag="VFU72_08965"
     CDS             complement(1332..1688)
                     /locus_tag="VFU72_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423657.1"
                     /translation="MPKTVLLVEDDPSLREVIAEILTLDGYEVLTASDGLEAIDILGL
                     RRPHIILLDMMMPRMDGFAFAAEITRRGLQIPTIVLTADNHAQQKAAQIGAEGYVEKP
                     FAIPTLLATVSRFVAN"
     gene            complement(1779..2384)
                     /locus_tag="VFU72_08970"
     CDS             complement(1779..2384)
                     /locus_tag="VFU72_08970"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU5423658.1"
                     /translation="MTAARTVGILIFDEVEVLDFCGPFEVFSIARAPEGGAESPPLFQ
                     ALTIAEEARTVTCRGGLLVQPHYDIHDHPPLDILLVPGGQGTRRERLNRRLLDWIAGQ
                     DRRSELTTSVCTGAFLLAEAGILSGRRATTHWASVDWMRQQYPAVTMLAETRVVDEAR
                     VITSAGVSAGIDMALHIVARLHGRETASWTARRMEYDWRPD"
     gene            complement(2481..>2948)
                     /locus_tag="VFU72_08975"
     CDS             complement(2481..>2948)
                     /locus_tag="VFU72_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5423659.1"
                     /translation="ESVERALDAERLEPDALRLELTESGVLAGSEAARTALRHLRRLG
                     IPLYLDDFGTGYSSLTAVLGAAVDGLKIDHTLVRLIDAEREGALVRGTLALARSLGLA
                     VVAEGIETPAQLTCLRELGCPNGQGFLFAPALEPGALEALIDAGDFALGRHPR"
BASE COUNT          403 a         1045 c         1026 g          474 t
ORIGIN      
        1 gcttggcgaa cagcgaggct ccgcgcagat aatcagcccg cgcggagccg ttggtgcggg
       61 agaagacgat gagtggggcg accaaagcgc gccaggggga gcaatccggc acgggggcgt
      121 cgggctggcg gctggccctc cagatcgggc tccagtcgct caaggatctg ctggtcgaca
      181 acgggccgca gtgggcggcc gctatcgcct actacactct cctctcggtc ttcccgctct
      241 tgctgctggc cgccgcgatc gccgccttct tcgtcgatcc ccagtgggcc gtcgatcgtg
      301 ccacgcatct gctcggcgac tatctgccgc aaggccacga cacggtgcgg caggtagtcg
      361 ccggtgcggt ggagcatcgc ggccagatcg ggttcctctc cgcgctcggc ctcctctggt
      421 cgggcacgcg cgtcttcgcc gcgctgacca aggcattgaa cgtcgccaac gggagcagcg
      481 cgcagtatgg cttcctggcg cgtctggtgg ttgaggcggc gatgctgctg acgatcggcg
      541 tggttctcac gctggcgctc atgtccggct cgctcttctc cctgctctgg ccgctgctgg
      601 agcacgcgcc gggcagtcct gggctggtga tcacgctggt ctcctgggcc atgcgggcgc
      661 tgttgctgat cggcgcctat ttcctggtct accagttcgt gccgcagggg aagcagcagc
      721 cgcgcgcctc gctgaccggc gcgctggtgg ccacggcgct ctttctgagc gccgatccca
      781 tcttccgcca ctatgtcggc aacagcggcc ggtacaacct ggtctacggc tcgctggcga
      841 tcatcatcat cgcgctgctc tgggtctgga tcgcggcgct gatcacgctc tttggcggcg
      901 aggttgccgc ccatacccgc gccatcgcca tcgagcggcg ctccccggcc gaggtcggcc
      961 ggcggcacgt ggcgcgcgcc cagccgccgc gcccgcgcgg agccgccacc gtgcccggcg
     1021 ccgcgcgcca gggcgccgcc caggccggca acctgctcga ccgcctcggt gatcgcatcc
     1081 agcgccttgt cgatcgcgtc agcggccagc agcgcgggcg ggtgtgaccg gcctgtcgat
     1141 tgagggtctg gtgctgcgtg ttgccgatga gagatccttc gctgcctaga gcggcccgca
     1201 cgatcgttga ccgccacccg cttcccctac aggcggggga caaccattgc ggcagacgct
     1261 ctcgtgcggg cgttccacgg cggcacgctg atcgatatgc ggccaaccgt ccaccgctcc
     1321 ggcgactggc gctagttcgc cacgaaccgg ctgaccgtgg ccaggagcgt ggggatggcg
     1381 aagggcttct cgacgtagcc ctcggcgccg atctgcgcgg ccttctgttg ggcgtggttg
     1441 tcggcggtca ggacgatggt ggggatctgg aggccgcgcc gggtgatctc cgcggcgaag
     1501 gcaaagccgt ccatgcgggg catcatcatg tcgagcagga tgatgtgcgg gcgccgcagg
     1561 ccgaggatgt cgatcgcctc cagcccgtcg ctggcggtca gtacctcgta gccgtcgagg
     1621 gtcagaatct cggcgatgac ctcacgcagg ctcgggtcat cctcgaccag cagcaccgtc
     1681 ttgggcacac ccaatccctc tcttctcccg cctccgcgcg ccgaatcggc acgccggcca
     1741 tcgcaatacg gagcccaacg gtcatcagcg acgggcggtt agtctgggcg ccagtcgtac
     1801 tccatgcgcc gggcggtcca ggacgcggtc tcgcggccgt gcagccgggc gacgatgtgc
     1861 agcgccatat cgatcccggc ggagacgccg gccgaggtga tcacgcgcgc ttcgtcgacc
     1921 acgcgcgtct cggccagcat ggtcaccgcc gggtactgct ggcgcatcca gtcgacgctg
     1981 gcccagtgcg tggtggcgcg ccggccgctc aggataccgg cctcggccag caggaaggcg
     2041 cccgtgcaga cactggtggt cagctcgctg cgccggtcct ggccggcgat ccagtcgagc
     2101 aggcgacggt tgagccgctc gcgccgcgtg ccctggccgc ccggcaccag caggatatcg
     2161 agcggcgggt gatcgtggat gtcgtagtgc ggttggacca gcaagccgcc gcggcaggtg
     2221 acggtgcgcg cttcctcggc gatcgtcaac gcctggaaga gcgggggtga ttcggcgccg
     2281 ccctccggcg cgcgggcgat cgagaagacc tcgaacgggc cgcagaaatc gagcacttcg
     2341 acctcgtcga agatcaggat cccaaccgta cgcgctgccg tcacgccctg cgtccttccc
     2401 ctgctccgcc gcctactctg cctgatgata cgcggaacac gcgacccgtg gggttggccc
     2461 cggtggtttc cgcgcccggc tcaacgtggg tgacggccga gcgcgaagtc gccggcgtcg
     2521 atcagcgcct ccagcgcgcc cggctccagc gcgggcgcga agagaaaacc ctgcccgttg
     2581 ggacagccca gctcgcgcag gcaggtgagc tgggcgggcg tctcgatccc ctcggccacc
     2641 accgccagcc ccaggctgcg cgccagcgcc agcgtgccgc gcaccagcgc gccctcgcgc
     2701 tccgcgtcga tcaatcgtac cagggtatgg tcgatcttca gaccgtcgac cgccgcgccg
     2761 agcaccgcgg tcagcgacga atagccggtg ccgaagtcgt ccaggtagag tgggatgccc
     2821 aggcgcctca gatggcgcag cgcggtgcgt gcggcctcgg agccggccag cacgccgctc
     2881 tcggttagct ccagccggag cgcgtcgggc tcgagcctct ccgcgtccag ggcgcgctcg
     2941 accgactc
//
LOCUS       DASUBI010000468         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_753342, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000468 DASUBI010000000
VERSION     DASUBI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753342"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFU72_08980"
     CDS             <1..307
                     /locus_tag="VFU72_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423660.1"
                     /translation="AGGAAGLVASGTGVVTAAAVASGFGVGIAVAIATVAAVEAGAEA
                     AVAGLWGAAPETAVAVLTRPAALVAAFSGFVLQPASASAAIKSRSIRTLSACPPPSQ"
     gene            770..1372
                     /locus_tag="VFU72_08985"
     CDS             770..1372
                     /locus_tag="VFU72_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5423661.1"
                     /translation="MASRHSIAQAREVSPWLARFDAVLRPGLRALELGCGLGHDAGEL
                     SAFGLAVCALDCEQARVARAHEEAPSAMLLVADLARTLPFSGEAFDLVVASLSLHYFD
                     RETTSRIVAEIARVLRPEGRLLCRVNAVGDVLHGYGQGAAREPELFEVAPGRTKRFFT
                     PESLTAFLQPGFVIDELTPRLAWTDTGEKRTLECLARRRG"
     gene            complement(1388..1867)
                     /locus_tag="VFU72_08990"
     CDS             complement(1388..1867)
                     /locus_tag="VFU72_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5423662.1"
                     /translation="MASSEDTATPPNETRQRIGPAIRTLRQEHHLSLSDLAQQTGISV
                     SYLSRLEKGKSVPSFTLLYRLAQVLGVDIGFFVETEKTATEIDQRLEQALSRTKLPRS
                     IWPELFSLSLTAREALLDFLEQLSPPVSGGANGATAQAHANRAGGGGRRRRADDKEG"
     gene            2113..>2967
                     /locus_tag="VFU72_08995"
     CDS             2113..>2967
                     /locus_tag="VFU72_08995"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU5423663.1"
                     /translation="MTSRYRSQLYPVLARLDAERAHRLALRALDSAQRVPGGSRLLRR
                     FAPAPDPRLRVERLGLVFPNPLGVAAGLDKNAEAVGALFALGFGAVEVGTITPRPQPG
                     NPPPRLWRFPAQQALVNALGFPSAGAASVRRRLTGCRFPGVVGVNLGKNRDTPPEHAA
                     ADYLAVLDALWDVTGYITVNVSSPNTPGLRDLQRPDALHALIRAVVERDALLARLHGG
                     RPGPVLVKLAPDLDETRLAEAVESLMAAGAAGVILGNTTTSRVGLPEAARELPGGVSG
                     APLRGRNLE"
BASE COUNT          411 a          992 c         1090 g          474 t
ORIGIN      
        1 tgcgggaggc gcggccgggc tcgtcgccag cggcaccggg gtcgtgaccg cggccgcggt
       61 ggccagcggc ttcggggtgg ggatcgccgt cgcgatcgcg acggtcgcgg ccgtcgaggc
      121 cggggcggaa gccgcggtag ccggactctg gggcgcggcg ccggagacgg cggtggccgt
      181 gctgacgcgg cccgccgcgc tggttgctgc gttctccggc ttcgtgctgc agcccgccag
      241 cgccagcgcc gcgatcaaaa gcaggagcat ccgcacgcta agcgcctgtc cgcctccgag
      301 ccaatgacgg gtgtcgcggt ggcccgtgcc gctgctgtcg tgtcggtcca cgcaacatct
      361 cctctactcg gtgcgccgcc gccggcgccg cactccctgg ctgcggagta gaaacgaacg
      421 cggacggtgt ccggcgccat ggatcgacgc ccgcaggttg ggatctgcag ctggtgggca
      481 gcgagccccg gggtgagtga gcaccgggca aggcgagccg gcgcggcgct cgaccatcag
      541 atacctcgat ccgttcccgg cagcgccgga aattgcccct tgccgaggtg aggccgccgg
      601 gattcgctgc gagcatccgg tgcccatccg gctgaggtcg cttgaaagaa atggtacgag
      661 attcgtcggg cgcggaggca gccgtacgcg ggcgacacgg gctggcaacc gttgccccga
      721 ctgagggttg ggggtatagt cgcgagcgct ggaggatagg gggagcgtga tggcgagtcg
      781 gcactcgatc gctcaggcgc gtgaagtcag cccgtggctg gcgcgcttcg acgcggtgct
      841 gcggccgggg ctgcgcgcgc tcgagctggg ttgtggtctg ggccacgatg ccggagagct
      901 atccgccttc gggctggccg tgtgcgcgct cgattgcgaa caggcgcggg tggcccgggc
      961 gcacgaagag gcgccgtccg ccatgctgct ggtggcggac ctggcgcgaa cgctgccctt
     1021 ctccggcgag gctttcgacc tggtcgtcgc cagcctctcg ctgcactact tcgatcgaga
     1081 gacgaccagc cgcatcgtgg ccgaaatcgc gcgcgtgctc cgccccgagg ggcggctcct
     1141 ctgccgggtc aacgcggtgg gcgacgtgct ccacggctac ggccaggggg ccgcgcgtga
     1201 gccggagcta tttgaggtcg ctccggggcg caccaagcgt ttcttcacgc cggagtccct
     1261 gactgcattc ctgcagcccg gcttcgtgat cgatgagctc acaccccggc tcgcctggac
     1321 cgataccggc gagaagcgca cgctggagtg cctggcgcgc cgccggggat gagccgggaa
     1381 tccccgctta tccctctttg tcgtcggcgc ggcgccgtct gcccccaccc ccggcgcggt
     1441 tggcgtgtgc ctgagcggtt gcaccgttcg cgccgccgga gacgggcggg ctcagttgct
     1501 ccaggaaatc gagcagcgcc tcgcgcgccg tcaggctcag gctgaagagc tcgggccaaa
     1561 tcgagcgggg gagttttgtc cggctgagcg cctgctccag gcgctgatcg atctcggtgg
     1621 ccgtcttctc ggtctcgacg aagaaaccga tatcgacgcc gagtacctgc gctagccggt
     1681 agagaagcgt gaacgaggga acgctcttgc ccttctccag ccgcgacaga tacgacaccg
     1741 agatccccgt ctgctgggcg agatccgaga gcgagaggtg atgctcctgg cgcagggtgc
     1801 ggatggccgg gccgatgcgt tggcgggtct cgttgggcgg agtcgcggtg tcttccgagg
     1861 atgccatcgc tgccaactcc attctgccgg acaggtcagc acagatgctg accggtgcgc
     1921 tcgaattcca gcaaagatac gacatactct aaaggtaagg cgcggcttgt gtcaagcgat
     1981 cttgcgagat tcatgaaacg cgccacgcgg cgaagcggac gcgactgccg gcctgctttc
     2041 aactagagta ccatgcgagc ggagacgcac cgtggagatt tcgttgagaa ctaggaggag
     2101 aggaacgctg gcgtgaccag ccgctatcgc tcgcagctct acccggtgct ggcccggctc
     2161 gacgccgagc gcgcccatcg cctggcgttg cgggcactgg attcggctca gcgcgtgccg
     2221 ggtggctcgc gtctgctacg ccgcttcgct ccggcccctg atccgcgcct ccgggtcgag
     2281 cgcttggggc tggtcttccc caatccgctt ggcgtggcgg ccgggctgga caagaacgcc
     2341 gaggcggtcg gggccctctt cgcgctcggc ttcggcgcgg tcgaggtggg cacgatcacg
     2401 ccgcgaccgc agccgggcaa cccgccgccg cgtctctggc gcttccccgc acagcaggcg
     2461 ctggtcaacg cgctcggctt ccccagtgcg ggcgcggcca gcgtgcggcg tcgcctgacc
     2521 gggtgccgct tccccggcgt cgttggggtc aatctgggca agaaccgcga caccccgccg
     2581 gagcacgccg ccgcggacta cctggccgtg ctcgacgccc tctgggatgt gacgggctat
     2641 atcacagtca acgtcagctc acccaacact cccggcctgc gcgatctcca gcggcccgac
     2701 gcgctgcacg ccttgatccg cgcggtcgtc gagcgcgatg ccctgctggc gcggctgcat
     2761 ggcgggcgtc caggtcccgt gctggtcaag ctggcgcccg atctggatga gacgcggttg
     2821 gcggaggcgg tcgagagcct gatggcggcc ggcgcggcgg gcgtgatcct gggcaacacc
     2881 accaccagcc gtgtcgggct gccggaggcg gcgcgcgagc tgccgggcgg cgtcagcggt
     2941 gctccactgc gcgggcgcaa tttggag
//
LOCUS       DASUBI010000469         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_543122, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000469 DASUBI010000000
VERSION     DASUBI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543122"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFU72_09000"
     CDS             complement(<1..678)
                     /locus_tag="VFU72_09000"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423664.1"
                     /translation="MVIQDTRTRRGPLGALVAAKLISLIGSQLTLVALPWFVLVTTGS
                     AAKTGLTGLAVTLPAVVVGLVGGALVDRLDYRQTSVIADLTSAAGIAAVPLLYHTRGI
                     EFWQLLILVFLGSLLTVPGLTARRAMLPELADLAGMTLDRANSISESASYVALLLGPP
                     LAGVLIAFIGASNVLWLDAASFVVSAAIVAGSIPAASQASERTVSGRYLDDLKAGLRF
                     VRDDRLLL"
     gene            complement(898..1200)
                     /locus_tag="VFU72_09005"
     CDS             complement(898..1200)
                     /locus_tag="VFU72_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5423665.1"
                     /translation="MQPNMKMLQQMQNKIMKMQEELGQTTVEGTAGGGAVTVTVNGLR
                     NVQGVKIAPEAVDPEDVEMLEDMILAAIQDAMTRAEELATQKMGAITGGLNIPGLM"
     gene            complement(1272..>2912)
                     /gene="recN"
                     /locus_tag="VFU72_09010"
     CDS             complement(1272..>2912)
                     /gene="recN"
                     /locus_tag="VFU72_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5423666.1"
                     /translation="GQRTSAELVRSGEASAFVEAVFDVEQIAARPELQALLQETGVEP
                     EDGTLVLTRDINASGRSTARVNGRTVTAATLAALGGLLVDIHGQSDHLSLLRPALHLD
                     LLDRYAGTLPEREALAGRVTQFQSVRRRIDAILSGERERAQRFDLLRFQVEEIGAARL
                     QPDEEESLERERAVLSNAERLARLAAEAYQLLEGGDLSAPEPAPGALDNLRLAVDRVE
                     ELASIDRDGEALAGPLREALFLLEDATQGVRAYAETVEADPARLEAIEDRLALFRQLK
                     RKYGAGLPEIIAFGERAAAELDTLEHGEEQADELRERAEELKRAIAAAAVALSEQRRA
                     AAGELEQAVEQAIAELNMGRARFVVDFGAHSGGEPVALTEHGQAREVRFDSTGIDRVE
                     FLIAPNAGEEPRPLARIASGGETARLMLALKSILSAADETPSLVFDEVDVGVGGRSGQ
                     PVGEKLWSLGDRHQVLVISHLPQVAAFAQAHYRITKIERAGRTETQVDRLDDTERLDE
                     LAAMLDGQPVTEASRENARAMLARIERWKRSAATAGVR"
BASE COUNT          397 a         1052 c         1020 g          443 t
ORIGIN      
        1 cagcagcagg cggtcgtcgc gcacgaagcg caacccggcc ttgagatcgt cgaggtagcg
       61 cccggagacc gtgcgctcgc tcgcctgcga cgcggccggg atgctgcccg cgacgatggc
      121 ggccgagacg acgaacgagg cggcgtcgag ccaaaggacg ttgctggcgc caataaaggc
      181 gatgagcacg ccggctagcg gcgggccgag caggagggcg acgtaagacg cgctctcgga
      241 gatgctgttg gcgcggtcga gcgtcatgcc ggctagatcg gccagctcgg ggagcatggc
      301 gcggcgcgcc gtcaggcccg gcacggtcag cagcgaaccg aggaacacga gtatcaggag
      361 ctgccagaac tcgatgccgc gcgtgtggta gaggaggggg acagccgcga tgccggccgc
      421 gctggtgagg tcggcgatga cgctggtctg gcggtagtcg agccgatcga ccagcgcgcc
      481 gccgaccagc ccgacgacga ccgccggcag cgtgacggcc aggccggtta gcccggtctt
      541 ggcggcgctg ccggtcgtga ccagcacgaa ccaggggagc gcgaccagcg tcaactggct
      601 gccgatcagc gagatcagct tggccgccac gagcgcgccg agcggcccgc gacgtgttcg
      661 agtgtcctgg atgaccacgc tgcggttccc ccgtcgcggg ctcttgtcct tgctcaacca
      721 ggaggccaga gctgagcttc gtcgggttcg gttagctcag ccggcccgtg cggcaggccg
      781 tccttcgcgt ccggggtaga cggccgcgcc aggcagagcg ccagcgtgcc gctccaacgg
      841 gatcacgata cccgctcgcg ccacccgtgc gcccccttgg ctcgctcgcc tggcctgcta
      901 cattaggccg gggatgttca gcccgccggt gatggcgccc atcttctgcg tggccagctc
      961 ctcggcccga gtcatggcgt cctggatcgc ggccaggatc atgtcctcca gcatctcgac
     1021 atcctcggga tcgaccgctt ccggggcgat cttgacgccc tgaacattgc gcaggccgtt
     1081 gactgtgacg gtcacggcgc cgccgcccgc ggtgccctcg acggtggtct gccccagctc
     1141 ctcctgcatc ttcatgatct tgttctgcat ctgctggagc atcttcatat tcggctgcat
     1201 gcggctcatc ctttcagaaa ttcatcgatc cgcccggccg gccacgcccg gcacttcgcg
     1261 tatatcacgg ctcagcgcac gccggccgtg gcggcgctcc gcttccagcg ctcgatccgc
     1321 gccagcatcg cgcgcgcgtt ctcgcgcgag gcctcggtga ccggttggcc gtcgagcatg
     1381 gcggccagct cgtcgagccg ctcggtgtcg tcgagccggt ccacctgcgt ctcggtgcgc
     1441 ccggcgcgct cgatcttggt gatacggtag tgcgcctggg cgaacgcggc aacctgcggc
     1501 aggtgactga tgactaacac ctggtgccgg tcgcccagcg accagagctt ctcgcccacg
     1561 ggctgcccgc tgcgcccgcc gacgccgacg tcgacctcgt cgaagaccag cgacggggtc
     1621 tcgtcggcgg ccgacaggat cgacttcagc gccagcatca agcgcgcggt ctcgccgccg
     1681 gaggcgatgc gcgccagggg acgcggctcc tcgccggcgt tgggtgcgat caggaactcg
     1741 acgcggtcga tgccggtgga gtcgaagcgg acctcgcgcg cctggccgtg ctcggtgagc
     1801 gcgaccggct cgccgccgga gtgcgcgccg aagtcgacca cgaaacgggc acgccccata
     1861 ttcagctcgg cgatcgcttg ctcgaccgcc tgctccagct cgcccgccgc cgcccgccgc
     1921 tgctccgaga gcgccacggc ggccgccgct atcgcgcgtt tcagctcctc ggcacgctcg
     1981 cgcagctcgt cggcctgctc ctcgccgtgc tccagcgtat ccagctccgc cgccgcgcgc
     2041 tcgccgaagg cgatgatctc gggcaggccg gcgccgtact tgcgcttgag ctggcggaag
     2101 agcgccaggc gatcctcgat cgcctccagc cgtgcggggt cggcttcgac tgtctcggcg
     2161 taggcgcgca ccccctgggt ggcgtcctcc agcaagaaga gcgcctcacg cagcggcccc
     2221 gccagggcct cgccatcgcg atcgatgctc gccagttcct cgacacgatc gaccgccagc
     2281 cgcaggttgt cgagcgcgcc gggggccggc tccggcgccg agaggtcgcc tccttcgagg
     2341 agctgatacg cctcggccgc cagccgcgcc agccgctcgg cgttgctgag cacggcacgc
     2401 tcccgttcga ggctctcctc ctcgtcgggc tgcagccggg ccgcgccgat ctcctctacc
     2461 tggaagcgca ggagatcgaa ccgctgggcc cgctcgcgct cgccgctcag gattgcgtcg
     2521 atgcgccgcc gcacgctctg gaactgggtc acgcgcccgg cgagcgcctc gcgctcgggc
     2581 agcgtgccgg cgtagcggtc gagcaggtcg aggtgcagcg cgggacgcag cagcgagagg
     2641 tggtcactct ggccgtggat atcgaccagc agaccgccga gcgcggccag cgtggcggcc
     2701 gtcacggtgc ggccgttgac gcgcgccgtg ctgcgtccgc tggcattgat gtcgcgggtt
     2761 aggaccagcg tgccgtcctc cggctcgacg ccggtctcct ggagaagcgc ctgaagctcc
     2821 ggccgggcgg cgatctgctc gacgtcgaag acggcttcga cgaaggcgct ggcctccccg
     2881 ctgcgcacca gctcggcgga cgtgcgctgg cc
//
LOCUS       DASUBI010000470         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_182385, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000470 DASUBI010000000
VERSION     DASUBI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182385"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFU72_09015"
     CDS             <1..652
                     /locus_tag="VFU72_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEU5423667.1"
                     /translation="KPAHAAADPYNGVNALNAVIEFFKGVDALRQHVPMETRIHGIIT
                     HGGDAVNVVPKYASADLLVRSPSVEGVNAVMDKVRKVAEGAALMTGCEYKITEGELHP
                     DMRPSYEIGRTYSDNMREMGIELNARAERGMHSTDFGHISYKIPSVTGSFAISHEPIP
                     GHSQQVVDASGSDFGYDQLIKVSKAMALTAFDLLTDPALLAQAKEERENWAERYEG"
     gene            824..2596
                     /locus_tag="VFU72_09020"
     CDS             824..2596
                     /locus_tag="VFU72_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5423668.1"
                     /translation="MTAANTPYPQALGGALLGEVSAERLMDYDRNIAQWVRLSGSEDE
                     AKAFDYVDKTLQSWGIQTERYAPVCLVSWPGPATLTILGSGQSLDCITHSFSTATGPD
                     GLTAEVVYAGGGAEADYAGKDVAGKIVLTEGLAGPAKVAPAERHGALGVINISGDIVH
                     EMIVSPVWGSPTPETVGELPHIPHISITTEAGERLKEELARGPVRVRITTEVDTAWRP
                     LPVLVGHVTPSRPSAFADEYVMFSGHIDSWHYGAMDNGSANATQMEVARILAGHRDLL
                     KRELRLAFWSGHSHARYGSSAWFADEFFYDLSDHCVAHVNIDGPGALNATVLTNAPTM
                     AESYPLAREVIQALSGQELAYRRIGRMGDQSFWGVGIPAFFCGLSSQGPSPESDALAA
                     GRSHSTGRRSGGTAWWWHTTEDTIDKIDPELLARDCRILLATVYRLVTDPVVALDQAA
                     AVDEIQRSLAEIAGAAGDAFDLAAVADEAEGLLAAARRLQARCQQPATDEQAHRLNAC
                     LMRLSRLLLPVNYTMHGPFEQDLALQYSSLPGLRPAATLAALPPHSQERHLLEVKLRR
                     ERNRVRHALREARATVEQTLAAVG"
BASE COUNT          423 a          955 c          872 g          347 t
ORIGIN      
        1 caagccggcg cacgcggcgg ccgaccccta caacggggtc aacgcgctca atgcggtgat
       61 cgagttcttc aagggggtgg acgcgctgcg gcagcacgtg ccgatggaga cgcgcatcca
      121 cggcatcatc acccacggcg gtgacgccgt gaacgtggtg cccaagtacg ccagcgccga
      181 cctgctggtg cgctcgccct cggtcgaggg ggtcaacgcg gtcatggaca aggtgcgcaa
      241 ggtcgccgag ggcgcggcgc tgatgaccgg ctgcgagtac aagatcaccg agggcgagct
      301 gcacccggac atgcgcccca gctacgagat cggccggacc tacagcgaca acatgcgcga
      361 gatggggatc gagctgaacg cccgcgccga gcgcggcatg cactcgaccg acttcggcca
      421 catctcctac aagatcccct cggtcaccgg ctcgttcgcc atcagccacg agccgatccc
      481 cggccactcg cagcaggtgg tggacgcctc gggctcggac ttcggttacg accagttgat
      541 caaggtctcc aaggccatgg ccctgaccgc cttcgatctg ctgaccgatc ccgctctgct
      601 ggcccaggcc aaggaggagc gcgagaactg ggccgagcgg tacgaggggt agaccctcac
      661 ccctgacccc tctcccgtgc gcgggagagg ggcactgccc atcgaaaacg tatcgctaca
      721 gggtagtgtt cctacgcctg atccgcgtct tcagccacct cccctcgccc ggcgcaccgg
      781 gagaggggcc gggggtgagg gcccgctaag gaggacgtca accatgaccg cagcgaacac
      841 tccctacccc caagcgctcg gcggcgcgct gctgggcgag gtctcggccg agcggctgat
      901 ggactacgac cgcaacatcg cccagtgggt gcgcctttcg ggcagcgagg acgaggccaa
      961 ggcgtttgat tatgtcgaca aaacgctgca gagctggggc attcaaaccg agcgctacgc
     1021 cccggtctgc ctggtcagct ggcccggccc ggccacgctg acgatcctcg gcagcggcca
     1081 gtcgctcgac tgcatcaccc actccttctc gaccgctacc gggccggacg ggctgacggc
     1141 cgaggtggtc tacgccggcg gcggcgccga ggccgactac gcgggcaagg acgtggccgg
     1201 caagatcgtg ctgaccgagg ggctggccgg ccccgccaag gtcgcccccg ccgagcgcca
     1261 cggcgcgctg ggcgtgatca acatcagcgg cgatatcgtg cacgagatga tcgtctcgcc
     1321 ggtctggggc agccccacgc ccgagacggt cggcgagctg ccgcacatcc cccacatctc
     1381 gatcacgacc gaggccggcg agcggctcaa ggaggagctg gcacgcgggc cagtgcgcgt
     1441 gcgcatcacc accgaggtcg acaccgcctg gcgaccgctg ccggtgctgg tgggtcacgt
     1501 cacccccagc cggccgagcg ccttcgccga cgaatacgtc atgttcagcg gccacatcga
     1561 ctcctggcac tacggcgcca tggacaacgg cagcgccaac gccacccaga tggaggtggc
     1621 gcgcatcctg gccggacacc gcgacctgct caagcgcgag ttgcgcctcg ccttctggtc
     1681 cggccactcg cacgcgcgct acggcagctc ggcctggttc gccgacgagt tcttctacga
     1741 cctgtccgac cactgcgtag cgcacgtcaa catcgacggc cccggcgcgc tcaacgccac
     1801 cgtgctgacc aacgcaccga ccatggccga gtcgtacccg ctggcgcgcg aggtgatcca
     1861 ggcgctcagc ggccaggagc tggcctatcg ccgcatcggc cgcatgggcg accagtcctt
     1921 ctggggcgtc ggcatcccgg ccttcttctg cggcctctcc tcgcaggggc cgtcgccgga
     1981 gtccgacgcg ctggccgccg ggcgctcgca cagcaccggc aggcgcagcg gcggcaccgc
     2041 ctggtggtgg cacactaccg aggacaccat cgacaagatc gaccccgagc tgctggcgcg
     2101 cgactgccgc atcctgctgg ccaccgtcta ccgcctggtg accgacccgg tcgtggcgct
     2161 cgatcaagcc gcggccgttg acgagatcca gcgatcgctg gccgagatcg ccggagcggc
     2221 gggcgacgcc ttcgacctcg ccgcggtcgc ggacgaggcc gaggggctgc tggcggccgc
     2281 gcgccggctg caagcgcgct gccaacaacc ggcgaccgac gagcaggccc accggctcaa
     2341 cgcctgcctg atgcgcctga gccgcctgct gttgccggtc aactacacca tgcacggccc
     2401 cttcgagcag gacctggcgc tgcagtactc ctcgctgccg gggctgcgcc cggccgccac
     2461 gctggccgcg ctgccgcccc acagccagga gcgacacctg ctggaggtca agctgcgccg
     2521 cgagcgcaac cgcgtgcgcc acgcgctgcg cgaggcccgc gcgacggtcg agcagacgct
     2581 ggcggcggtt ggatagc
//
LOCUS       DASUBI010000471         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_586607, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000471 DASUBI010000000
VERSION     DASUBI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586607"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFU72_09025"
     CDS             complement(<1..131)
                     /locus_tag="VFU72_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5423669.1"
                     /translation="MPLSGVRVLDLGRHLAGPTAALWLGDLGADVIKIEKPKVGEDGR
                     "
     gene            462..1832
                     /locus_tag="VFU72_09030"
     CDS             462..1832
                     /locus_tag="VFU72_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEU5423670.1"
                     /translation="MSQQGQGQGPEPPERHRHTFGFDTRAIHAGQRPDPYTGARAMPI
                     YQTTSFVFEDTEAAAAYFDLQEYGHIYSRIGNPTVAAFEERVASLEGGIGAVAFASGL
                     AAQAAALFTLLQPGDHLVASQALYGGTTAQFKNVLGKMSIETTFVNPDDLDAWQAALR
                     PNTKALYGETIGNPGGNVLDLAAVAAIAHRHGAPLVVDNTFASPYLCRPIEWGADIVI
                     HSATKFIGGHGTSIGGIVVDSGTFEWENGRFPAMVEPSANYHGLPFIDTFGMYGYLMK
                     LRAETLRDLGASMSPFNAFMFLVGLETLPLRMERHVENALGVARFLAAHPMVERVHYG
                     GLPESPYFPLVEKYLPNGAGAIFAIDIKGGRPAGKRFIESLRLWSHLANVGDAKSLVI
                     HPATTTHRQLTDEELLAGGISAGTIRLSVGLETLEDLLWDLDQALAAARAETPAAVAT
                     AAGGDA"
     gene            1829..2335
                     /locus_tag="VFU72_09035"
     CDS             1829..2335
                     /locus_tag="VFU72_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5423671.1"
                     /translation="MSEQQTAPAAFDIRKYQDARTMRDTLANYRNIAIVGLSSNPLRG
                     SYFVAYYLKRHGYHIIPVNPNETEVLGEKAYPTLSDVPGPIDVVDVFRAPEHAPAVAR
                     EAVKVGAKVLWLQFGVISPEAAEIAERGGLQVVMDRCMKVEHARTLGRMHWMGFNTEG
                     ISAQRGEV"
BASE COUNT          367 a          903 c          795 g          358 t
ORIGIN      
        1 cggccgtcct cgccgacctt cggcttctcg atcttgatca cgtcggcgcc taaatcgccc
       61 agccacaggg ccgccgtcgg cccggccaga tgccgcccca agtcgagcac gcgcaccccg
      121 ctcagcggca tgccgagccc gctcgcgccg ttgctgcgct acttgtccat tggttctccc
      181 tcctgtcgtc gcacgttgtg cctgcacgcg ggccgggtcc tcatccccca cggggagccc
      241 tcatcccccc ccgccccttc tcccaacgcg ttgggagaag gggagctcgg tacgggcgtg
      301 ccttcgtagc ccctctccca agattgggag aggctccgtg cgggttgggg tgaggtctcc
      361 ccgcccggtt gccgccgcgc ccccacctgc gtaggatagc gcgtgtggcc tccgcgcgcc
      421 gactgccagc cacgcacgca accaggacgg gagcagcagc catgagtcag cagggtcagg
      481 gacagggacc cgagccgccc gagcggcacc gccacacctt cggcttcgac acgcgcgcca
      541 tccacgccgg ccagcggccg gacccctaca ccggcgcgcg cgccatgccg atctaccaga
      601 ccaccagctt cgtcttcgag gacaccgagg ctgccgccgc ctacttcgac ctccaggagt
      661 acggccacat ctacagccgc atcggcaacc cgaccgtggc tgccttcgag gagcgcgtcg
      721 ccagcctgga gggcggcatc ggcgcggtcg ccttcgccag cgggctggcc gcccaggcgg
      781 ccgccctctt caccctgctg cagcccggcg accatctggt cgcctcgcag gcgctctacg
      841 gcggcaccac ggcccagttc aagaacgtgt tgggcaagat gtcgatcgag acgaccttcg
      901 tcaaccccga cgacctcgac gcctggcagg ccgcgctgcg ccccaacacg aaggcgctct
      961 acggcgagac gatcggcaac cccggcggca acgtgctcga cctcgcagcc gtggccgcca
     1021 tcgcccaccg ccacggcgcg cccctggtgg tggacaacac cttcgcctcg ccctacctct
     1081 gccgcccgat cgagtggggc gccgacatcg tgatccactc ggccaccaag ttcatcggcg
     1141 gccacggcac ctcgatcggc ggcatcgtgg tcgactccgg caccttcgag tgggagaacg
     1201 ggcgcttccc ggcgatggtg gagccatcgg ccaactacca cggcctcccc ttcatcgaca
     1261 ccttcggcat gtacggctac ctgatgaagc tgcgcgccga gaccctgcgc gatctgggcg
     1321 cctcgatgag ccccttcaac gcgttcatgt tcctggtggg gctggagacg ctgccgctgc
     1381 gcatggagcg gcacgtcgag aacgcgctgg gggtggcccg cttcctggcc gcgcacccga
     1441 tggtcgagcg cgtccactac ggcggcctgc ccgagagccc ctacttcccg ctggtcgaga
     1501 agtacctgcc aaatggcgcc ggcgccatct tcgccatcga catcaagggc ggccgcccgg
     1561 ccggcaagcg cttcatcgag tcgctgcggc tctggagcca cctggccaac gtcggcgacg
     1621 ccaagagcct ggtgatccac ccggcaacca ccacccatcg ccagctgacc gacgaggagc
     1681 tgctggccgg cggcatcagc gcgggcacca tccggctctc ggtcgggctg gagacgctgg
     1741 aggatctgct ctgggatctg gatcaggcgc tggcggcggc gcgcgcggag acgccggcgg
     1801 cggtcgcgac tgcggctggg ggtgacgcgt gagcgagcag cagacggcgc cggccgcgtt
     1861 cgacatccgc aagtaccagg acgccaggac gatgcgcgac acgctggcga actaccgcaa
     1921 catcgccatc gtcgggctct cctccaaccc cctgcgcggc agctacttcg tcgcctacta
     1981 cctcaagcgg catggctacc acatcatccc ggtcaacccc aacgagaccg aggtgctggg
     2041 cgagaaggcc tatcccacgc tctccgacgt gcccgggccg atcgacgtgg tcgacgtctt
     2101 ccgcgcgccg gagcacgcgc cggcggtggc gcgcgaggcg gtcaaggtgg gcgccaaggt
     2161 gctctggctg cagttcggcg tgatcagccc ggaggcggcc gagatcgccg agcgcggcgg
     2221 gctgcaggtg gtgatggacc gctgcatgaa ggtggagcac gcgcgcaccc tcggccgcat
     2281 gcactggatg ggcttcaaca ccgagggcat cagcgcgcag cgcggggagg tctagccggc
     2341 gacgctgctt gggagctgtc gtgacttgtg gtgccgcggc gttcccggtg ttcaggatct
     2401 gggttgttgc cgcgcaaaca gct
//
LOCUS       DASUBI010000472         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_423499, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000472 DASUBI010000000
VERSION     DASUBI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423499"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFU72_09040"
     CDS             complement(<1..216)
                     /locus_tag="VFU72_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5423672.1"
                     /translation="MPHPLVLQLRFARDEFRRGLDGVSEAEARRRFMPMNCMSWNVGH
                     LAWQEQRYWLQFAQGRLLLPEINRDFAY"
     gene            532..1053
                     /locus_tag="VFU72_09045"
     CDS             532..1053
                     /locus_tag="VFU72_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423673.1"
                     /translation="MKRGNVLLWGFAGTTVLTTILRLGQAAGLTRIDLPLILGLIFTQ
                     DRDRAKAYGFLTHFVNGWIFAGIYAATFRSLRRTSWWIGGTVGLIHGLFVLTAGLPVL
                     PGVHPRMASENQGPEPTSELEPPGFMAINYGRETAIATLVAHVIFGIILGTFLGAPAS
                     SKSRWFQRLTATR"
     gene            complement(1029..>2040)
                     /locus_tag="VFU72_09050"
     CDS             complement(1029..>2040)
                     /locus_tag="VFU72_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5423674.1"
                     /translation="RNWDYRFCWLRDAAFTIRALLRLGYQTEARAFAAWLLYATRLTH
                     PQLQVMYTVFGHARIPERILAYLRGYRGSRPVRFGNAASEQLQLDIYGEVIDALALYH
                     QAGGPFDQDARGVLEGLVQVILRRWREPDDGIWEPRSGVAQHVHSKLMALVGLDRALA
                     LAHGGALRLPAHVPRERDAIRDWLLREGFDAHRQTFTSVPGGGLDAALLVMPLIDFLP
                     PDDQRVVGTIEAIQRELAEGALVYRYHAADGLSGHEGAFVLCSFWLVEALAKIGRLDE
                     AHAHFRRLLAYRNDLGLMAEELDPVTGEQLGNFPQAFSHIGLINAALTLADMDQRVAV
                     NR"
BASE COUNT          307 a          686 c          721 g          326 t
ORIGIN      
        1 ataggcgaag tcgcggttga tctccggcag gagcaggcgc ccctgagcga actggagcca
       61 gtagcgctgc tcctgccagg ccaggtggcc gacgttccag ctcatgcagt tcatgggcat
      121 gaagcggcgc cgcgcctccg cttcgctgac gccgtcgagg ccgcgacgga actcgtcgcg
      181 ggcaaagcgg agctgcagga ccagtgggtg aggcatatgc gtgttcgctc ctcgttgttc
      241 gggccgtccg ccacagtcga gtcgttccag catcgcgttg gcacgaccgc ggcaggccag
      301 ggccgcgacg gtgtgcgcgg gccgagcgcg gatatcctct tgttgccgca gcctagaaca
      361 tacgagctac tctgtcaagg cgccgtcggc cacaccctct cgtccgctcg cgcgtgccag
      421 tcgtgacctt ggtgtaggct gtacctgccg gcggttggtg cgcaaccgtc gccgacgagc
      481 gcgcgccggt cgagcgggag tccttcggct ggcggtttgg agaagagagc gatgaagcgg
      541 ggcaatgtgc tcttgtgggg gttcgccggc acgacggtgc tgaccaccat cttgcggctg
      601 ggacaggcgg ccggcctgac ccgcatcgat ctgcccctca tcctcgggct gatcttcacg
      661 caggaccggg accgcgccaa ggcctacggc ttcctcaccc acttcgtgaa cggctggatc
      721 ttcgccggca tctatgccgc caccttccgc tcgctgcggc gcacgagctg gtggatcggc
      781 ggcacggtcg gcctgatcca cggcctgttc gtgctgacgg ccgggctccc cgtcctaccc
      841 ggggtccatc cccgcatggc gtcggagaac cagggcccgg agccgaccag cgagctggag
      901 ccgccggggt tcatggcgat caactacggg cgtgagacgg ccatcgccac tctggtagcg
      961 cacgtcatct tcggcatcat cctgggcacc ttcctgggcg ccccggcatc atcgaagtcg
     1021 cgctggtttc agcgattgac tgccactcgc tgatccatgt ccgcgagcgt gagggccgcg
     1081 ttgatcaggc cgatgtggct gaatgcctgc gggaaattgc ccagttgctc acccgtcacc
     1141 ggatcgagct cctccgccat caaccccaga tcgttgcggt aggcgagcag gcgccggaag
     1201 tgcgcgtggg cctcgtcgag gcggccgatc ttggccagcg cctcgacgag ccagaacgag
     1261 cagaggacga acgcgccctc gtgcccgctg agcccgtccg ctgcgtggta gcggtagacg
     1321 agcgccccct cggccagctc gcgctggatc gcctcgatgg tgccgaccac gcgctggtcg
     1381 tccggcggca ggaagtcgat gagcggcatc accagcagcg cggcgtcgag cccgcctccg
     1441 ggaacgctgg tgaaggtctg ccgatgtgcg tcgaagccct cgcgcagcag ccagtcgcgg
     1501 atggcgtccc gctcgcgcgg cacgtgtgcc ggcagccgca gcgcgccgcc gtgcgccagc
     1561 gccagcgccc gatcgagtcc caccagggcc atcagcttgg agtgcacgtg ctgggccact
     1621 cccgagcgtg gctcccagat gccgtcgtcc ggctcgcgcc agcgccgcag gatgacctgc
     1681 accaggcctt ccagcacacc gcgcgcgtcc tggtcgaacg ggccgccggc ctggtgatag
     1741 agcgccaggg cgtcgatgac ttcgccgtag atgtcgagct ggagttgctc ggaggccgca
     1801 ttgccgaagc gtaccggccg ggagcctcga tagccccgca gataagccag gatacgctcg
     1861 gggatgcggg cgtggccgaa gacggtgtac atcacctgga gctgggggtg cgtcaggcgg
     1921 gtggcgtaga ggagccaggc ggcgaaggcg cgcgcttccg tctggtagcc gaggcggagg
     1981 agcgcgcgga tggtgaaggc cgcgtcgcgc agccagcaga agcggtagtc ccagttgcgc
//
LOCUS       DASUBI010000473         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_847279, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000473 DASUBI010000000
VERSION     DASUBI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847279"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..363)
                     /locus_tag="VFU72_09055"
     CDS             complement(148..363)
                     /locus_tag="VFU72_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU5423675.1"
                     /translation="MTTKLSISLPDDDLEFLDELNADSRSAAIHAAIELARSLSLVDD
                     YTAAYDEWVESGDAELWEAVVGDGLTQ"
     gene            complement(473..1804)
                     /gene="clpX"
                     /locus_tag="VFU72_09060"
     CDS             complement(473..1804)
                     /gene="clpX"
                     /locus_tag="VFU72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5423676.1"
                     /translation="MARIDNADLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNEI
                     IEEEPGEPAAARGLAELPKPKEIYDFLDEYVIGQENAKKSLSVAVYNHYKRVQAGDYQ
                     RERGGRRGEHIELAKSNILLLGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVG
                     EDVENILLKLIQAADYDIKKAETGIIYIDEIDKIARKAENPSITRDVSGEGVQQALLK
                     ILEGTVSSVPPQGGRKHPHQEFLQIDTTNVLFIVAGAFEGLDKIVDARVNKRGMGFTA
                     QLRSRYDIDTTDVFADVLPEDLLKYGMIPEFVGRLPVVTSVHKLDRAALVRILVEPRN
                     ALIKQYQRLMELDGVELVFEDDAVEAIADQAILRGTGARGLRAIMEEVLMSVMYEVPS
                     HSDVGTVVITGEVVREHVLPTLVPRDQRGLNEHGRRTGTQQSLNERRERSA"
     gene            complement(2043..>2579)
                     /locus_tag="VFU72_09065"
     CDS             complement(2043..>2579)
                     /locus_tag="VFU72_09065"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5423677.1"
                     /translation="LFDERIIFLGVQIDDASANDVMAQLITLESMDPDRDISIYINSP
                     GGSFTALTAIYDTMQWVKPDIQTVCMGQAASAAAVLLAAGTPGKRLALPNARILIHQP
                     WSEGGRGDLSDLEIQANEILRMRNVMEQMLAKHSGRDADQVHQDIDRDKILSAEEAKD
                     YGIIDQVLTSRTETLNAR"
BASE COUNT          367 a          854 c          875 g          483 t
ORIGIN      
        1 cccctcggcc gctccgatgc gccgcggtgt tcacaccatc gttggagacc acgatcgcgg
       61 ggcgggtctt ggtgatctcg cttccgcgcg ccgggtccat gtcggcccag aacacgtcgc
      121 ctcgccgaat ggcttgcacc gcgccgttca ctgagtgagc ccgtcgccga ccacggcttc
      181 ccacagttcg gcgtccccgg attcgaccca ttcgtcgtag gcggcggtgt agtcgtcgac
      241 caggctcagg gagcgggcca gttcgatagc ggcatggatc gccgccgacc gagagtcagc
      301 gttcagctcg tccaggaact cgaggtcgtc atccggtagg gagatgctca gcttcgtcgt
      361 catggagata tcttaccctg gtatgcagca cggtactact cgagtagtac tcgaaagagt
      421 gaagcggcgg cccgccgaag cgaaccgccg ccttgccgtc tgtgaaagtg ccctacgccg
      481 accgctcccg ccgctcgttc aggctctgct gcgtcccggt ccggcgacca tgctcattca
      541 aaccgcgctg gtcgcgcggc accagcgtgg gcagcacgtg ctcgcgcacc acctcgccgg
      601 tgatgaccac ggtgccgacg tcggagtggc tcggaacctc gtacatcacc gacatcagca
      661 cctcctccat gatcgcgcgc aggccgcggg cgccggtgcc gcgcaggatc gcctggtcgg
      721 cgatggcctc cacggcgtcg tcctcgaaga ccagctccac gccgtcgagc tccatcaggc
      781 gctggtactg cttgatcagc gcgttgcgcg gctcgaccag gatgcggacc agggccgcgc
      841 ggtccagctt gtggaccgag gtgaccaccg gcaggcggcc gacgaactcc gggatcatgc
      901 cgtacttcag caggtcctcc ggcagcacgt cggcgaagac gtcggtggtg tcgatgtcat
      961 agcgggagcg cagctgggcg gtgaagccca tgccgcgctt gttcacccgg gcgtccacga
     1021 tcttgtccag gccctcgaag gcgccggcga cgatgaacag cacgttggtg gtgtcgatct
     1081 gcaggaactc ctggtgcggg tgtttgcgcc cgccctgcgg cggcaccgag gagaccgtgc
     1141 cctccaggat cttcagcagc gcctgctgca cgccctcgcc ggagacgtcc cgggtgatcg
     1201 aggggttctc ggccttgcgg gcgatcttgt cgatctcgtc gatgtagatg atcccggtct
     1261 cggccttctt gatgtcgtag tcggccgcct ggatcagctt gagcaggatg ttctccacgt
     1321 cctcgccgac gtagccggcc tcggtgagcg cggtggcgtc ggcgatggcg aaggggacgt
     1381 tgagcatccg ggccagggtc tgggccaggt acgtcttgcc gcagccggtg gggcccagca
     1441 gcaggatgtt gctcttggcc agctcgatgt gctcgccccg gcgcccgccg cgctcgcgct
     1501 ggtagtcgcc ggcctgcacc cgcttgtagt ggttgtagac cgccaccgac aagctcttct
     1561 tggcgttctc ctggccgatg acgtactcgt cgaggaagtc gtagatctcc ttgggcttgg
     1621 gcagctcggc caggccccgg gccgcggccg gctcgccggg ctcctcctcg atgatctcgt
     1681 tgcacagatc gatgcactcg tcgcagatgt atacgccggg gcccgcgatg agcttcttga
     1741 cctgcttctg actcttgccg cagaaggagc acttcagcag gtcggcgttg tcgatgcgtg
     1801 ccacggtgga gcttctcctt tgtgaagatc ccggtgctgg acccactact ggacgcctgg
     1861 aggcgcttcg tagtctgtcc cccaagacgg taccgctcat acccccggcg aggcgtgagc
     1921 gctcgccacg tgcgagcgca tcggccgtgg catcgaatga taatccgatg ccacggcgcg
     1981 cggtgggtca tccggcgccg aggccgtgcc cgattcggat ccggcggcgc gccgcgtcgg
     2041 cctcagcggg cgttcagcgt ctcggtgcgg ctggtcagga cctggtcgat gatgccgtag
     2101 tccttcgcct cctcggccga gaggatcttg tcccggtcga tgtcctggtg cacctggtcg
     2161 gcgtcgcggc cggagtgctt ggccagcatc tgctccatga cgttgcgcat ccgcaggatc
     2221 tcgttggcct ggatctccag gtccgacagg tcgccgcggc cgccctcgct ccagggctgg
     2281 tgaatcagga tccgggcatt gggcagcgcc aggcgcttgc ccggggtgcc ggcggccagc
     2341 agcacggcgg ccgcggaggc ggcctggccc atgcagaccg tctggatgtc cggcttgacc
     2401 cactgcatgg tgtcgtagat ggcggtcagc gcggtgaacg agccgccggg gctgttgatg
     2461 tagatggaga tgtcccggtc ggggtccatc gactccagcg tgatcagctg ggccatcacg
     2521 tcgttggccg aggcgtcgtc gatctgcacg ccgaggaaga tgatccgctc gtcgaacag
//
LOCUS       DASUBI010000474         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_5005, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000474 DASUBI010000000
VERSION     DASUBI010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5005"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..913
                     /locus_tag="VFU72_09070"
     CDS             65..913
                     /locus_tag="VFU72_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423678.1"
                     /translation="MKIALLTERLRVEERLLLDAFAARGHEASVVAPSQLSVNLARPL
                     APAWPLALQRAPAAVEHAALLALLAAQGIVVVNRPATARLLADRLALVRHLVFAGIPV
                     PETIASFGEAATFAAIETLGYPVLLKSLSVDITPLALIEDRDAAEAVIEHRGVLGGEQ
                     AVLVQRFAAGQGRSERLAIAGSTLLGVETRSHHGWRPGREAPYAPYDGDLSAHRALAQ
                     RLIERLGSGCYSIEVVAGEDGLSVVGAENLTDFRSLSEAGVDVAGAIADYALEQARGA
                     KDASGG"
     gene            906..2009
                     /locus_tag="VFU72_09075"
     CDS             906..2009
                     /locus_tag="VFU72_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HEU5423679.1"
                     /translation="MVEQLSRQETGVAALLEGLVRVPSYSGQEAEAVAWLCDQMRALG
                     YRARPDGAGNAVGTIGSGPREIMLLGHIDTVPGAPPVRVADGALYGRGTVDAKGPLAT
                     FVVAGARARLPEGVRLTVIGAVEEEVMSSRGAHFLVDQREAPDAVIIGEPGGWEGIVL
                     GYKGSLSLTYRVTHHVSHSAGPEPTAGELGVAFWNRLSAWCLARNDGAEHGFETVDCT
                     LNSINTSSDGLQAACELRGGLRLPPSLPPEEAAAALETLAQGGELAIAINAAAFRGDK
                     RSPLVAALLAAIRTVGGSPKLKLKTGTSDMNLVGPAWGCPIVAYGPGDSRLDHQPDEH
                     VPLADLEHGVAVLTAAIERIAGQLASGRWGGNA"
     gene            2006..2971
                     /gene="lysX"
                     /locus_tag="VFU72_09080"
     CDS             2006..2971
                     /gene="lysX"
                     /locus_tag="VFU72_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEU5423680.1"
                     /translation="MNDDLRIGVLMSHVRAEEKQLLAAFAKRGAEPVRLLDRHLAFEL
                     TAPEQAQLGPLDVVLDRAMAHGRAAVALQCFDALGVRCINSSTASGIADDKVATTLTL
                     SLAGVPNIRTYAAFDVESALRVLDERVGYPAVIKPITGSWGRLLAKVNTPEAARAVLE
                     HKKALGSFHHGVFYIQEYVEKPGRDLRIFVIDGQVIAGSYRTAEHWVTNVARGAVSRP
                     CPITSEIEELAVRATGALGLELAGVDLVESSDGLQVIEVNTGVEFKGLIRTTDIDIPG
                     VIADYVVRAARQEHAARGDLVGALNGRRPAQRQVLGQDGVVPHDD"
     gene            3194..>3414
                     /locus_tag="VFU72_09085"
     CDS             3194..>3414
                     /locus_tag="VFU72_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5423681.1"
                     /translation="MALDVAILGGSGYAGGELLRLLLGHPEVNVTQVTSRGRAGKFVH
                     TVHPNLRKRTQLKFIPPDKLEKVDVLFAAL"
BASE COUNT          492 a         1227 c         1227 g          468 t
ORIGIN      
        1 gacgacccgc tgacgctcga cctggcgccc gccgaagagg aagactgggg cgagtaggcc
       61 gccgatgaag atcgcactgc tcaccgagcg cctgcgcgtc gaggagcgcc tgctcctcga
      121 cgccttcgcc gctcgcggcc acgaagcgag cgtggtcgcc ccctcgcagt tgagcgtcaa
      181 cctggcgcgt ccgctggctc ccgcgtggcc gctggcgctg caacgcgcgc ccgccgccgt
      241 cgagcacgcg gccctgctgg ccctgctggc ggcgcagggt atcgtcgtgg tcaaccggcc
      301 ggccacggcg cgcctgctgg ccgaccggct ggcgctggtg cgccacctcg tcttcgccgg
      361 catcccggtg ccggagacga tcgccagctt cggcgaggcg gccacctttg ccgccatcga
      421 gacgctcggc tatccggtcc tgctcaagtc actcagcgtc gatatcacgc cgctggcctt
      481 gatcgaggat cgcgacgccg ccgaggcggt gatcgagcac cgcggcgtgc tggggggcga
      541 gcaggcggtg ctggtgcagc gcttcgccgc cggccagggc cgctccgagc ggctggcgat
      601 cgccggcagc acgctcctcg gcgtcgagac gcgcagccac cacggctggc gcccggggcg
      661 cgaggcgccc tacgcgccct acgacggcga cctgagcgcc caccgggcgc tggcccagcg
      721 tctgatcgag cgcctcggca gcggctgcta cagcatcgag gtagtggccg gcgaggacgg
      781 gctctcggtg gtcggcgccg agaacctgac cgacttccgc tcgctcagcg aagcgggtgt
      841 cgacgtggcc ggcgcgatcg ccgactacgc gctggagcag gcgcgcggcg cgaaggacgc
      901 cagcggtggt tgagcagctc agccggcagg agaccggcgt cgccgcgctc cttgaagggc
      961 tggtacgcgt ccccagctac tccggccagg aggccgaggc cgtcgcctgg ctctgcgacc
     1021 agatgcgcgc gctcggctac cgggcccggc cggacggcgc ggggaacgcc gtcgggacca
     1081 tcggctccgg cccgcgcgag atcatgctgc tgggccacat cgacaccgtg ccgggcgcgc
     1141 cgccggtgcg cgtagcggac ggcgcgctct acgggcgcgg cacggtcgac gccaaggggc
     1201 cgctggcgac cttcgtggtg gccggcgcgc gggcgcgcct tcccgagggc gtgcgcctga
     1261 cggtgatcgg ggcggtcgag gaggaggtga tgtcctcacg tggggcgcac ttcctggtcg
     1321 atcagcgtga ggcgcccgac gccgtgatca tcggcgagcc gggcggctgg gaggggatcg
     1381 tcctcggcta caagggctcg ctcagcctga cctatcgcgt gacccaccac gtctcgcact
     1441 cggccggtcc cgagccgacc gccggcgagc tgggcgtggc cttctggaat cgcctgagcg
     1501 cgtggtgctt ggcgcgcaac gacggagccg agcacggctt cgagaccgtc gactgcacgc
     1561 tcaatagcat caacaccagc tccgacggcc tgcaggccgc ctgcgagctg cgcggcgggc
     1621 tgcgcctgcc gccgtcgctt ccgccggagg aggcagccgc cgcgctggag acgctggcgc
     1681 agggcggcga gctggccatc gcgatcaacg cggcggcctt ccgcggcgac aagcgctcgc
     1741 cgctggtggc ggccttgctg gccgccatcc ggactgtggg gggcagcccg aagctcaagc
     1801 tcaagaccgg cacctcggac atgaacctgg tcggccccgc ctggggctgt ccgatcgtgg
     1861 cctacgggcc gggcgattcc cgcctggatc accagccgga cgagcacgtc ccgctggcgg
     1921 acctggagca cggcgtggcg gtgctcaccg ccgccatcga gcgcatcgcg ggacagctag
     1981 cgagtggacg ttggggaggg aacgcgtgaa cgacgaccta cgcatcggcg tgctgatgtc
     2041 gcacgtccgg gcagaggaga aacagctcct ggcggccttc gccaaacgcg gggccgagcc
     2101 ggtgcgcctg ctcgaccgcc acctggcctt cgagctgacc gccccggagc aggcgcaact
     2161 ggggccgctc gatgtcgtgc tcgaccgcgc catggcccac gggcgcgcgg cggtcgcgct
     2221 gcagtgcttc gacgccctcg gcgtgcgctg catcaacagc tcgaccgcct ccggcatcgc
     2281 cgacgacaag gtcgccacca cactcaccct ctcgctggcc ggggtgccca acatccgcac
     2341 ctacgccgcc ttcgacgtcg agtcggcgct gcgcgtgctc gacgagcgcg tcggctaccc
     2401 cgccgtgatc aagccgatca ccggctcctg gggccggttg ctggccaagg tcaacacgcc
     2461 cgaagcggcg cgcgcggtgc tggagcacaa gaaggcgctc gggtcgttcc accacggcgt
     2521 cttctacatc caggagtatg tcgagaagcc ggggcgcgac ctgcgcatct tcgtcatcga
     2581 cggccaggtg atcgccggct cctaccgcac cgccgagcac tgggtcacca acgtggcgcg
     2641 cggcgcggtc tcgcgcccct gcccgatcac ctcggagatc gaggagctag cggtgcgcgc
     2701 caccggcgcc ctcggcctgg agctggccgg cgtcgatctg gtcgagagct cggacggcct
     2761 gcaggtgatc gaggtcaaca ccggggtcga gttcaaaggg ctgatccgca cgaccgacat
     2821 cgacatcccc ggcgtgatcg cggactacgt ggtgcgcgcc gcgcggcagg agcacgccgc
     2881 gcgcggcgac ctggtcggcg cgctgaacgg ccgtcgcccc gcccagcgcc aggtgctcgg
     2941 ccaggacggc gtggtgccgc atgatgacta gtcgccctgc ctcgcccact gtcggggcag
     3001 gcccccgtgt ctgccctgtc tcccctacgg catcgcgacg gggacatgga gagcgtgggc
     3061 ggcgtcgatg aggatcggca gtaaggcaac gagggcagac acgggggtct gcccctacag
     3121 tcgagaactg agcggcacca gaccagaaca acgcgcgaca catgcatatt ttgagtagga
     3181 gaagacgaga tcaatggcac tcgacgttgc tatcctgggc ggctccggct acgccggggg
     3241 tgagttgctg cggctactgc tcgggcatcc ggaggtcaac gtgacccagg tgacctcgcg
     3301 cggccgcgcc ggcaagttcg tccacaccgt ccaccccaac ctgcgcaagc gcacccagct
     3361 caagttcatt ccgcccgaca agctggagaa agtcgacgtc ctcttcgcgg ccct
//
LOCUS       DASUBI010000475         8298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1287120, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000475 DASUBI010000000
VERSION     DASUBI010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287120"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..293)
                     /locus_tag="VFU72_09090"
     CDS             complement(39..293)
                     /locus_tag="VFU72_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423682.1"
                     /translation="MGTLAGEAVELEPAGEAAAEAAADFVGAEVAAAALVAVAPPAPL
                     ALVELPAPEVAAGVLELPPQAASKLSNATPVKPSPAMRIS"
     gene            307..1512
                     /locus_tag="VFU72_09095"
     CDS             307..1512
                     /locus_tag="VFU72_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5423683.1"
                     /translation="MPTIAAGSHSLKIVLWSHFVPAYDTWFDKWAKDWGSSNKVDVTV
                     DHIPTNTVPTRMSAEVSANSGHDLVQFQNGSDVHVFSDKLNDVTDLSGYLASKYGGWA
                     EVAKDAAVVNGAWKGVPQYLIRFPSMYRKDLFDQEGLQAPKVWEDLINVGEKLKPKGH
                     PVGFAISQTGDSNSTLYSILWSYGGSVTGKDGKTITIKSDGTKAALEAVQKIFKAGMT
                     NEVLSWDDSSNNRYLASGVACYILNPISAYRSAPQDIQDKTAIALTPAGPAGDFVNCP
                     IYTWAIPTWTKEKPAAEKFLVDFYADWKAGFEASTGYNHPLLLDMTKKPMPVLGADPK
                     LQVLQDFLPLARTIGYPGPATAAAGQVNNTYVIPNMFAKAVTGSSADDAMAWAEEQIK
                     AVYDKYPTT"
     gene            complement(1787..1933)
                     /locus_tag="VFU72_09100"
     CDS             complement(1787..1933)
                     /locus_tag="VFU72_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423684.1"
                     /translation="MVTGGCGCGCCAYFLLVLALIGAGIVWVYRWLRGREEQELPTQE
                     PTRF"
     gene            2198..3703
                     /locus_tag="VFU72_09105"
     CDS             2198..3703
                     /locus_tag="VFU72_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423685.1"
                     /translation="MPDGAKPAAANRTATERAIEAGITPGTHKWFVLSVVSVGTFMAT
                     LDGSIVNISLPTIQSAFGVSLSAIEWVVVAYLLTVGTLLLPFGRAGELLGYKRVYVTG
                     FVFFTAASVLCGLSLTIWMLVVFRIIQGIGASMLQAMGPAIVAATFGPRERGRALGLN
                     SVSVSVGLSVGPTIGGILTEYGSWRWIFYINLPVGIFAILWAMRVLERERHGGQDQFD
                     IRGATLQLAALLSLLLALIEGQSWGWTSSLVVGLLAAFVVFGAAFIMTELRSPHPMLD
                     LRLFKIRPFSAGNGALMISFAALFTATFLMPFFLIQGEGFSTLRAGLLLTPIPLTTMI
                     FAPLSGALSDRIGQRIPATAGLAVMALGLYLLTGIRIGMPTWDLVVRLMVIGVGQGLF
                     TSPNSSAVLGSVPRARLGTASATLAQMRIDGQAVGIAAAGAIVAAQTAAYVAKLSGTL
                     APELVQREAFVHAIHDAFFVAAIVCLFAILASMVRGPRLEEEIGPANTNHG"
     gene            3997..5202
                     /locus_tag="VFU72_09110"
     CDS             3997..5202
                     /locus_tag="VFU72_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU5423686.1"
                     /translation="MPDTRRMHLKPTSRAVHAGERPAPRDFVPTATPIYATSSFVYQD
                     IEQMDAALGGATGVFVYGRYGNPTLEAMETAVAALEETDAALGVASGMAAVNLPILAT
                     APRGGRVVASRDVYGATVKLLDQIYRDLGIETVFVDILDLDAVRAGLARGDVRAVVCE
                     TISNPLLRVTDIPAVAKLAHAVGAALIVDNTFATPWLVRPAGLGADYVVHSSTKYLGG
                     HGDVTAGVIASDAERRLQLNELNKAVGSVLSPFDAWLILRGIKTLPLRLVRQSENALT
                     VARWLAEQPKVARVFYPGLPGHESHQTANRLFPDGLYGAMVSFEMHDCDRAAVVRFLG
                     ALEMIVPATTLGDVFSLVLYPVMSSHRALTPEQRETVGIGESLVRLSVGIEDPADVIA
                     DLERALSVV"
     gene            5235..6419
                     /locus_tag="VFU72_09115"
     CDS             5235..6419
                     /locus_tag="VFU72_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5423687.1"
                     /translation="MTATQSKIAMIREQMPATAKYAYLNTGTFGPLPRVTVEAISGYL
                     NDELEEGRLSDKAGYRRGAEAKAAARQEMSKIFNCSPDNVVLTRHTTDGMNIGILGLN
                     WRHGDELIITSMEHPGGQVPAYLVGHRFGVTVRTANLGDGSGDVVGKLERLVNPRTRM
                     IVTSHLTWNTGAILPLKEIVDMAHRHHLLVVVDGAQSTGSIPLNVEQLGADVYAGPGQ
                     KWLCGPEGTGATYVSDEAMEQLQPTVAGYMSGRLEHRGGYFLPHHGAMRYEVGGSFSP
                     AILGQATSVHFIRETVGLDWVYKRISELGKYCWKSLAALDGVNVLTPMECMAGLVSFT
                     VDGLDPNEVVKKLAEREVVIRSIHDPDATRASTGFYNTEEDIDNLAAGIKAIQQEEKG
                     KA"
     gene            6416..7207
                     /locus_tag="VFU72_09120"
     CDS             6416..7207
                     /locus_tag="VFU72_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5423688.1"
                     /translation="MSARDNGSGGLLLWELSWPEIREIKNRVDLVLLPVGSNEQHGPN
                     LAVNMDIASCSEFCRRASARMYPRVAVAPPVPWGVSFHHMNFPGTITLSTETFIQVLV
                     EVIASLQHHGFERFLIVNGHGGNVPAMNVATVRAREELQVPFVGACSYFSFGDREITQ
                     KYVTSPITGHACEVETARAMALVPQVVKHGALAKGEITELQSGFGQLARKYNVSIPYK
                     FDQLTRNGALGDATKATEEFGRELLESALGNFTAFCDELIKANPV"
     gene            7567..>8298
                     /locus_tag="VFU72_09125"
     CDS             7567..>8298
                     /locus_tag="VFU72_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5423689.1"
                     /translation="MKSGFEAELEQLESRVVALYGIAEAVARRQRDRLAVSEGEIHLD
                     LYPPQREQTGTVVFVGGLSSHALMYAGFLDALSRRGHAVVALDLRGHGRSSGERGDFT
                     IESVIEDLAAAAGYARQRFGGPVTVLGSSLGGFFALVGANAVEGFDAGISHGVLLPEL
                     PITAKDRRLAPLARLLARAAPRLRLSTRLLTDWGGVAESPELRAAIQADPLMAWRYTT
                     RAIASGSSYRPARPLTDLRVPHLLIV"
BASE COUNT         1375 a         2947 c         2620 g         1356 t
ORIGIN      
        1 aacgagcggc cgttgcggcc gaattcactc agtcgccgtc aactgatgcg catcgccggg
       61 cttggtttga ccggagtggc gttggagagc ttgctcgccg cctgcggcgg cagctccagc
      121 actccggccg cgacctcggg cgcgggcagc tcgaccaagg ccagcggcgc gggcggcgcc
      181 accgcgacga gcgccgccgc cgcgacctcg gccccgacga aatccgccgc ggcctcggcc
      241 gccgcctcgc ccgccggctc cagctcaacg gcctcgccgg ccagtgttcc cacctcgaat
      301 tatcaggtgc cgacgatcgc cgccggctca cactcgctca aaatcgtgct ctggagccac
      361 ttcgtgccgg cctacgatac ctggttcgat aagtgggcca aggactgggg ctcctcgaac
      421 aaggtcgacg tcaccgtcga ccatatcccg accaacaccg tcccgacgcg catgtccgcc
      481 gaagtctcgg ccaacagcgg ccacgatctg gtgcagttcc agaacggctc ggacgtccat
      541 gtcttctccg acaagctgaa tgacgtgacc gatctgtcgg gctatctcgc gtccaagtac
      601 ggcggctggg ccgaggtggc aaaagacgcg gcggtggtaa acggcgcctg gaagggcgtg
      661 ccgcagtacc tgatccgctt ccccagcatg taccgcaagg acctcttcga ccaggagggt
      721 ctgcaggctc ccaaagtatg ggaagacctg atcaacgtcg gagagaagct caagcccaag
      781 gggcacccgg tcggcttcgc catctcgcag accggcgact cgaactccac gctctactcg
      841 atcctctgga gctacggcgg ctcggtcacc ggcaaggacg gcaagacgat caccatcaag
      901 tcggacggga ccaaagccgc gctggaggcg gtgcagaaga tcttcaaggc ggggatgacg
      961 aacgaggtcc tctcctggga tgactcctcg aacaaccgct acctcgcctc gggcgtggcc
     1021 tgctacatcc tgaacccgat cagcgcctac cgctcggcgc cgcaagatat ccaggacaag
     1081 accgccatcg cgctcacgcc cgccgggccc gcgggcgact tcgtcaactg cccgatctac
     1141 acctgggcca ttccaacctg gaccaaggag aaaccggccg ccgagaaatt cttggtcgac
     1201 ttctacgccg actggaaggc gggcttcgaa gccagcacgg gctacaacca cccgctgctg
     1261 ctcgacatga ccaagaagcc gatgccggtt ctgggcgcgg acccgaagct ccaggtcttg
     1321 caggacttcc tgccgctggc gcgcaccatc ggctaccccg gaccggccac cgccgccgcc
     1381 ggccaggtga acaacaccta tgtgatcccg aacatgttcg ccaaggccgt caccggcagc
     1441 tcggccgacg acgccatggc ctgggccgag gagcaaatca aggcggtcta cgacaagtat
     1501 ccgacgacgt agcgaatcgg ggagtcctca tccccctgcc ccttctccca aaactggggg
     1561 aaggggcgct atggggagaa cgactggcgt acgacgatcg ccgccgtccg gaaggccaca
     1621 ctcccagccc ttgccatccc ctccttcgac aggctcagaa ccaggctcag agcgggctct
     1681 gagcttgtcg aaggatccgt cctcatgggg cgctgatcga agttgattgc tgctgacagc
     1741 accggagtct ctcctctccg ggtttggaac tgtgcctccg agcgcgtcag aaccgcgtgg
     1801 gctcctgggt cgggagctcc tgctcttccc gcccgcgcag ccaacgatag acccagacga
     1861 tccccgcgcc gatcagcgcc aagacaagca gaaaataggc gcagcaacca cagccgcagc
     1921 cgcctgtcac catctcgtcg tcctcccttc gcggcgcgtc atccgcggat acgatgccgc
     1981 cggagacgcc gctctcccca ggttagcggc ctcctcgagc atcgtcacct gtcccggcac
     2041 gccgggccgg tggaaagcgg actgcgtgac gccgcggccc gccgactcgc cgggccgcgg
     2101 cgaaggggaa tcgtagccag accacagacg ttttgatctg acgtacgcac cactggccgc
     2161 gattcgctcg aaatcgcgga tgcacgaggg gagcgtcgtg ccagacggag cgaagccagc
     2221 cgccgccaac cgcacggcca ccgagcgcgc catcgaggcg ggcatcaccc ccggcaccca
     2281 caagtggttc gtgctctccg tcgtctcggt cggcaccttc atggcgacgc tcgacggcag
     2341 tatcgtcaac atctcccttc cgaccatcca atcagcgttc ggcgtctccc tctcggccat
     2401 cgagtgggtc gtcgtcgcct acctgctgac ggtcggcacc ttattgctgc cgttcggccg
     2461 cgccggtgag ctgctcggct acaagcgcgt ctacgtcacc ggtttcgtct tcttcaccgc
     2521 tgccagcgtc ctctgcgggc tctccctcac catctggatg ctggtcgtct tccgcatcat
     2581 ccagggaatc ggcgcctcga tgctgcaggc tatgggaccg gcaatcgtag cggcaacctt
     2641 cgggccgcgt gagcgcggac gtgcgctggg gttgaacagt gtcagtgtgt cggtgggact
     2701 gagtgtcggc ccgactatcg gcggcattct taccgagtac ggttcctggc gctggatctt
     2761 ctacatcaac ctgccggtcg gcatcttcgc catcctgtgg gccatgcgcg tgctcgagcg
     2821 cgagcggcac ggcggtcagg accagttcga tatccgcggc gccacactcc agctcgccgc
     2881 cctgctctcg ctgctgctgg cgctgatcga gggccagtcc tggggctgga ccagctcgtt
     2941 ggtcgtcggc ctcctggccg ctttcgtggt tttcggcgcg gctttcatca tgacggagct
     3001 gcgctcgccg cacccgatgc tcgacctgcg cctcttcaag atccgcccct tctcggccgg
     3061 caacggcgcg ctgatgatct ccttcgccgc cctcttcacc gccaccttct tgatgccgtt
     3121 cttcctgatc cagggcgagg gcttctcaac cctcagggcc ggtctgctgc tgacgccgat
     3181 cccgctgacg accatgatct tcgcgccgct tagtggcgcg ctctccgacc gcatcggaca
     3241 gcgtattccg gccacggccg gcttggcggt catggcgctc gggctctatc tgctaaccgg
     3301 catccggatc ggcatgccca cctgggatct ggtcgtccgt ctgatggtga tcggcgtcgg
     3361 gcaaggcctc ttcaccagcc ccaacagcag cgcggttctc ggctcggtgc cgcgtgcccg
     3421 gctgggtacc gcctcggcaa ccctggcgca gatgcgcatc gacggccagg cggtcggcat
     3481 cgccgcggcc ggagcgatcg tcgccgcgca gaccgccgcc tacgtggcga agctcagcgg
     3541 cacgctggcg ccggagctag tgcagcgcga ggccttcgtt catgccatcc acgacgcctt
     3601 cttcgtcgcc gcgatcgtct gcctcttcgc catcctggcc agcatggtgc gtggcccgcg
     3661 cctggaggaa gaaataggac cagccaacac caatcatggc tgagcacggc gccaggaccc
     3721 gctggtgttg gcccgcgttg cctgcccatc gaggagcatt gacgccgagt gaggacggat
     3781 tctttcagga tgacagggat aagaaggggc ggacgacaag gacacgcgtc gtacgctcca
     3841 atccctcaaa catgtcatct tgagcttgtc gaaggaaccg tcctcgacag ccacaacgtg
     3901 tgcttgtcgg cctcgatccc tacctccgtt tgacagccca tgacgcctgc cgctatagtg
     3961 ccgggctgtc tgacgccgtc ccaagaggga gccgtgatgc ccgacacccg tcgcatgcac
     4021 ctgaaaccga ccagccgcgc cgtccacgcc ggtgagcgcc cggcgccgcg cgacttcgtt
     4081 cccaccgcca ccccgatcta cgccacctcc agcttcgtct accaggacat cgagcagatg
     4141 gacgccgcgc tcggcggcgc caccggcgtc ttcgtctacg gccgctacgg caacccgacc
     4201 ctggaggcga tggagacagc ggtcgcggcg ctggaggaga ccgacgcggc gctgggggtt
     4261 gcctccggca tggccgcggt caacctgccg atcctggcca ccgctccgcg cggcggccgc
     4321 gtcgtcgcct cgcgcgacgt ctacggcgct accgtcaagc tgctcgacca gatctaccgt
     4381 gacctgggca tcgagaccgt cttcgtcgac atcctcgacc tcgatgcggt ccgggccggg
     4441 ctagcgcgcg gcgacgtgcg cgcggtcgtc tgcgagacga tctccaaccc gctcttgcgg
     4501 gtgaccgata tcccggcggt cgccaagctg gcgcacgccg tcggcgcggc gctgatcgtc
     4561 gacaacacct tcgccacacc ctggctggtg cggccggccg gtttgggcgc cgactacgtc
     4621 gtccatagct cgaccaagta tctcggcggg cacggtgacg tcaccgccgg cgtgatcgcc
     4681 agcgacgccg aacgccgcct gcagctcaac gagctgaaca aagcagtcgg cagcgtactc
     4741 agcccgttcg acgcctggtt gatcctgcgc ggcatcaaga cgctgccgtt gcgccttgtg
     4801 cgccagagtg agaacgcatt gaccgtggct cgttggctgg ctgagcagcc gaaggtcgcg
     4861 cgcgtcttct accccggctt gcccggccac gagagccacc agaccgccaa tcgtctcttc
     4921 cccgacggcc tctacggcgc gatggtctcc ttcgagatgc acgactgcga ccgcgcggcc
     4981 gtcgtccgct tcctcggcgc gctggagatg atcgtcccgg ccaccacgct cggcgacgtc
     5041 ttcagcctgg tgctctatcc ggtgatgtcc tcgcatcgcg cgctgacgcc ggagcagcgc
     5101 gagacggtcg gcatcggcga gtcgctggta cgcctctcgg tgggcatcga ggacccggcg
     5161 gacgtcatcg ccgacctgga gcgtgcgctc tcagtcgtgt aatggctgta atgggttcgt
     5221 caaaggagga acaggtgacc gcgacgcaat cgaagatcgc tatgattcgg gagcagatgc
     5281 cggcgacggc caaatacgcc tacctcaaca ccggcacctt cggcccgctg ccgcgggtca
     5341 cggtcgaagc gatctccggc tacctcaacg acgagttgga ggagggccgc ctctccgaca
     5401 aggcaggcta ccggcgcggc gcggaggcca aggccgccgc gcgccaagag atgagcaaga
     5461 tcttcaactg ctcgccggac aacgtggtgc tgacgcgcca caccaccgac ggcatgaaca
     5521 tcggcattct cggactcaac tggcggcatg gcgacgaact gatcatcacc agcatggagc
     5581 accccggcgg gcaggtgccg gcctatctgg tcggccaccg cttcggcgtg accgtccgca
     5641 ctgccaatct cggcgacggc tccggcgacg tggtcggcaa gctcgagcgc ctggtcaacc
     5701 cgcgcacgcg catgatcgtc accagccacc tgacctggaa caccggggcc atcctgccgc
     5761 tcaaggagat cgtcgacatg gcgcaccggc accacctgct ggtggtcgtc gacggcgcgc
     5821 agtcgaccgg ctcgattccg ctgaatgtcg agcagctcgg cgctgacgtc tatgcggggc
     5881 cgggccagaa gtggctctgc ggccccgagg gcaccggcgc cacctacgtc tcggacgagg
     5941 cgatggagca gttgcagcca acagtcgccg gctacatgtc cggccggctg gagcaccggg
     6001 gcggttactt cctgccgcac cacggcgcca tgcgctacga ggtcggcggc agcttctcgc
     6061 ccgccatcct gggccaggcc accagcgtcc atttcatccg cgagacagtc gggctcgact
     6121 gggtctacaa gcgcatcagc gagctgggca agtactgctg gaagtcgctc gccgcgctgg
     6181 acggcgtcaa cgtgctgacg ccgatggagt gtatggccgg gctggtcagc ttcaccgtgg
     6241 acggcctgga tccgaacgag gtggtgaaga agctcgccga gcgcgaggtc gtcatccgct
     6301 cgatccatga cccggatgcc acccgtgcct cgaccggctt ctacaacacc gaagaggata
     6361 tcgacaatct ggcggccggg atcaaggcga ttcagcagga ggagaagggc aaagcatgag
     6421 cgcgcgcgac aacgggtccg gcgggctgct tctctgggag ctctcctggc cggagatccg
     6481 ggagatcaag aaccgggtgg atctcgtcct cttgccggtc ggctccaacg agcagcacgg
     6541 gccgaatctg gcggtcaaca tggacatcgc ctcctgctcc gagttctgcc gccgggcctc
     6601 ggcgcgcatg tacccgcgcg tcgcggtcgc gccgccggtc ccctggggcg tctccttcca
     6661 ccacatgaac ttccccggca cgatcacact ctcgaccgag accttcatcc aggtgctggt
     6721 ggaggtcatc gcctcgctgc agcaccacgg cttcgagcgc ttcctgatcg tcaacgggca
     6781 cggcggcaac gtgccggcca tgaacgtggc gacggtgcgg gcacgtgagg agctgcaggt
     6841 tccgttcgtc ggcgcgtgct cttatttcag ctttggcgac cgcgagatca cccagaagta
     6901 cgtcacctct ccgatcaccg gccacgcctg cgaggtcgag acggcgcggg ctatggcgct
     6961 ggtgccgcag gtcgtcaagc acggcgcgct ggccaagggg gagatcaccg agctgcagag
     7021 cgggttcgga caactggcgc gcaagtacaa cgtcagcatc ccctacaagt tcgaccagtt
     7081 gacgcgcaac ggcgcgctcg gtgacgccac caaggcgacg gaggagttcg gccgcgagct
     7141 gttggagagc gcgctaggca acttcaccgc cttctgcgat gagctgatca aggcgaaccc
     7201 cgtgtagcac ccggacacag cacccagcac cacgccagga ccgcccccgg gcggtcctgt
     7261 ttgcatccat aaacagcgcc cggttgcagc ccgccgactc catggactgc gccgcgctta
     7321 tgcccatgcg tctaatgcgc cagggcgacc aggggggtcg tcccaaccgg ggaacgtgtc
     7381 ttggtactcc caatgcatcg gcaacagggg caacgacgca tcctccaccg gtaggggcag
     7441 gcccccgtgc ctgctctcgc gccccgccac caacccctca gagtcgccag ccgatgtcgc
     7501 cccttggccc catcgctccg gcggccgtga tcgacgatac tgtgtatgag caaggaagtg
     7561 aggggaatga agtccggatt tgaagccgag ctggagcaac tggagtcccg cgtggtcgcg
     7621 ctctacggta tcgcggaagc cgtcgcacgc cgccaacgcg accggctggc cgtctccgag
     7681 ggcgagatcc acctcgacct ctatccgccg cagcgcgagc agaccggcac ggtcgtcttc
     7741 gtgggcggcc tcagctcgca cgcgctgatg tacgccggtt tcctcgacgc gctcagccgg
     7801 cgcgggcacg cggtcgtcgc gctcgacctg cgtggccacg gacgcagcag cggggagcgc
     7861 ggcgacttca ccatcgaatc cgtgatcgaa gacctcgcgg ccgcggccgg ctacgcccgc
     7921 cagcgctttg gcgggccggt taccgtgctc ggctccagcc tgggcgggtt cttcgcgctg
     7981 gtcggcgcca acgccgtcga gggcttcgac gccggcatca gccacggcgt cctgctgccc
     8041 gagctgccga tcaccgccaa ggatcgacgt ttggcgccgc tggcgcgact gctggcccgc
     8101 gccgcgcccc gcctgcgtct cagcacgcga ctgctcaccg attggggcgg ggtggccgag
     8161 tcgcccgagc tgcgcgccgc catccaggcc gacccgctga tggcctggcg ctacaccacg
     8221 cgcgccatcg ccagcggctc cagctaccgc ccggcgcgcc cgctgaccga cctgcgcgtg
     8281 ccgcacctgc tgatcgtt
//
LOCUS       DASUBI010000476         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_97957, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000476 DASUBI010000000
VERSION     DASUBI010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97957"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFU72_09130"
     CDS             <1..933
                     /locus_tag="VFU72_09130"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEU5423690.1"
                     /translation="VPLIAPNQSMVTTWESPLKKVIDRAREMEREPGVVAATVLGGFS
                     FSDTPFTGLAMIVVTDDDKALAQRYADELAALAWQLRDDFEIHPTPIADAVAEAMAGE
                     PGSVYVLADISDSGASGTAGDGTAVLKGLLEANAKSASVAQIMDQQAVDDCIAAGVGS
                     TVTLSVGGKHDGLHGKPVEVTGMVRLIHEGRFVMGGPMGTGTTASRGRTVVLEIGGRD
                     GIELQLTDLRGHPNDLNFFRAFGIEPTQRRILVLKSAAHFRAAFEPIATKVIEVDAPG
                     ISSPKLDTFDYQRLSRPRYPLDKDFDWSPPRRES"
     gene            complement(1124..>3620)
                     /locus_tag="VFU72_09135"
     CDS             complement(1124..>3620)
                     /locus_tag="VFU72_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEU5423691.1"
                     /translation="IDLRGVRVACLSGENGAGKSALLDCITWALWGKARVNNDRQLMA
                     LNASEMEVVFSFLLNGQEFRVLRRRGRGGNGPLTLDLQVRDDGRWRSISGATSRETQQ
                     AIDHLLCMDYDTFINSSFILQGRADEFTTKTPASRKQVLADILNLSEYDRLEELARAE
                     FRERDRQLRELDLELARLERELESAPQHEAEVARLSQVILELGTQIDSHDGRLQTVRR
                     LLQALEVTAEQRERLLGEIRSLDAEIEELAGLRAGTARRIEEQRAVLARRQEIEDSYR
                     RLLELRRRNEELTTQMAARQSLLERRRAIERVITGQEHEIASEQRSLEDRRAALAALI
                     AGREALTAQLDQVRKQTAALPILKREIDDTRAEQSALETQRGELQAENRQLKSEMSAL
                     KARMDQIGEEDAACPVCRRPLGEHERHDIRADYVSEGTVLGDRYRANQTRLKEIEAEL
                     KQHADDLAALERQRTKLDSLCRSEATLQERLARGDEAATEETSLAKRADDLLAQIAAG
                     QIAAAERAELARIESELHGLAYDHDEHLALQEQVKALHGVEAEVQQLLVASTALAKDA
                     EQLDHINAQAARCRDERARRVEQEAELAAQVAQIDEHRALHDELEALLDRLVRERSLA
                     QEAFGAAQERLAACRKLEEEREQRLTERGRTVEEKALYDELTLAFGKRGIQAMVIENI
                     VPELQDEANAILDKMPGNTMRIEFQTQRQALSGDSTIETLDIIIGDEAGRRPYELYSG
                     GEAFRANFAIRVALSTLLARRAGARLQTLVIDEGFGTQDSRGRDGLIEAIHAIEDDFA
                     MILVITHIAELKDLFPTRIDVVKTGDGSLVSVN"
BASE COUNT          515 a         1228 c         1235 g          642 t
ORIGIN      
        1 gtcccgctga tcgctcccaa ccagtcgatg gtcaccacct gggagtcgcc gctgaagaag
       61 gtcattgacc gggcgcgcga gatggagcgc gagccgggcg tggtcgcggc cacggtgctc
      121 ggcggcttct cattctccga cacccctttc accggcctgg cgatgatcgt ggtgacggac
      181 gacgacaagg cgctggcgca gcgctacgcc gacgaactgg ccgcgctggc ctggcaactg
      241 cgcgacgact tcgagatcca ccccaccccg atcgccgatg ctgtcgccga ggccatggcc
      301 ggcgagccgg gcagcgtcta cgtgctggcg gatatctccg attccggcgc cagcggcacg
      361 gccggcgacg gcacggcggt cctcaagggc ctgctggagg cgaacgccaa gtctgcttcg
      421 gtggcgcaga tcatggacca gcaggcggtc gacgactgca ttgccgcggg cgtgggcagc
      481 acggtcacgc tctcggtcgg cggcaagcat gatggtctgc acggcaagcc ggtcgaggtg
      541 accggcatgg tgcgcctgat ccatgaggga cgcttcgtga tgggcggtcc gatgggcacc
      601 ggcacgaccg ccagtcgtgg ccgcaccgtg gtgctggaga tcggcgggcg cgacgggatc
      661 gagctgcagt tgaccgatct gcgcggccac cccaatgacc tgaacttctt ccgcgccttc
      721 ggcatcgagc cgacccagcg ccgcatcctg gtgctcaaga gcgcggcgca cttccgcgcg
      781 gccttcgagc cgatcgcgac caaggtgatc gaggtggacg cgccgggcat cagcagcccg
      841 aagctggaca ccttcgacta ccagcggctg agccggccgc gatacccgct cgacaaggac
      901 ttcgactggt ctccgccgag gcgcgagtcg tagggcgcgc cccatcgagt tgattaagtg
      961 cccagtgagg acggatcctt cgacaagctc aggatgacag ggttatggaa gggcggacga
     1021 cgacgactgc ctttgtccgg aaggccacaa ccgtagaccg tcatcctgag cttgtcgaag
     1081 gatccgtgcc gcacccggca cccaacgccg tcgatggccg ccgttagttc acactcacca
     1141 gcgagccgtc gccggtcttg acgacgtcga tgcgggtcgg gaacagatct tttagctcgg
     1201 cgatgtgggt gatcaccagg atcatggcga agtcgtcctc gatagcgtgg atcgcctcga
     1261 tcagtccgtc gcgcccgcgg ctgtcctgag tcccgaagcc ctcgtcgatc accagcgtct
     1321 gcaggcgcgc gccagcgcgg cgggctagca gcgtgctgag cgcgacccgg atagcgaagt
     1381 tcgcgcgaaa ggcctcgcca ccgctgtaca gctcgtaggg gcggcggccg gcttcgtcgc
     1441 cgatgatgat gtcgagcgtc tcaatggtgc tgtcgccgga gagcgcctgg cgctgggtct
     1501 gaaactcgat acgcatggtg ttgccgggca tcttgtcgag gatggcgttg gcctcgtctt
     1561 gcagctcggg aacgatgttc tcaatgacca tcgcctggat gccgcgcttg ccgaaggcca
     1621 gcgtcaactc gtcgtagagc gccttctcct cgacggtgcg gccccgttcg gtgagccgct
     1681 gctcgcgctc ctcctccagc ttgcggcagg ccgccaggcg ctcctgcgcc gcgccgaagg
     1741 cctcctgcgc cagcgatcgc tcacgtacca ggcgatcgag cagggcttcc agctcgtcgt
     1801 ggagcgcgcg gtgctcgtcg atttgagcca cctgcgccgc cagctcggcc tcctgctcga
     1861 cgcgcctggc gcgctcatcg cggcagcgcg ctgcctgcgc gttgatatgg tcgagttgtt
     1921 ccgcgtcctt ggccagcgcg gtgctggcga ccaaaagctg ctggacctcc gcctcgactc
     1981 catgcagcgc cttgacctgt tcctgcagcg ccaggtgctc gtcgtggtcg taggcgaggc
     2041 catgcaactc gctctcgatc cgggccagct cggcccgctc cgcggcggcg atctgaccgg
     2101 ctgctatctg ggcgagcagg tcgtccgccc gcttggccag ggacgtttcc tcggtggcgg
     2161 cttcgtctcc ccgtgccagt cgctcctgga gggtcgcctc gctacggcac aggctgtcga
     2221 gcttggtgcg ctggcgctcc aacgcggcga ggtcgtcggc gtgctgcttc agctccgcct
     2281 cgatctcctt cagccgtgtc tggttggcgc ggtagcgatc tccgagcact gtaccctcgg
     2341 acacgtagtc cgcccggatg tcgtggcgct cgtgctcgcc gagcgggcgc cggcagaccg
     2401 ggcaagcggc atcctcctcg ccgatctggt ccatgcgcgc cttgagggcg ctcatctcgg
     2461 atttgagctg gcggttctcg gcctgcagct ctccccgctg cgtctccagc gccgattgct
     2521 cggcgcgcgt gtcgtcgatc tcacgcttca agatcggcag cgccgcggtt tgcttgcgca
     2581 cctggtcgag ctgcgcggtg agtgcctcgc gcccggcaat caacgcagcc agggcggcgc
     2641 ggcgatcctc cagggagcgc tgctcgctcg cgatctcgtg ctcttggccg gtgatgacgc
     2701 gctcgatggc gcggcgccgc tcgagcaacg actggcgagc ggccatttgg gtcgtgagct
     2761 cctcgttgcg ccggcgcagc tcgagcagac gccggtagct gtcctcgatc tcctgacgtc
     2821 tggccaggac ggcgcgctgc tcctcaatgc ggcgcgcggt gccggcgcgc agaccggcca
     2881 gttcctcgat ctccgcgtcg agcgagcgga tctcgcccag caggcgctcg cgctgctcgg
     2941 cggtcacctc gagggcttgt aggagtcgac gcaccgtttg caggcgtccg tcgtggctgt
     3001 cgatctgcgt gcccagttcg aggatcacct ggctgagacg cgccacctcg gcctcgtgct
     3061 ggggcgcgga ttccagctcg cgctccagcc gcgccagctc caggtcgagc tcgcgcagtt
     3121 ggcggtcgcg ctcgcggaac tcggcgcgcg ccagctcctc cagccggtcg tactcgctca
     3181 ggttcaggat gtcggccagc acctgcttgc ggctagccgg cgtcttggtg gtgaactcgt
     3241 ctgcgcgtcc ctggaggatg aaggacgaat tgataaaggt gtcgtagtcc atgcagagca
     3301 ggtgatcgat ggcctgctgg gtctcgcggc tggttgcacc gctgatcgag cgccagcgac
     3361 cgtcgtcgcg aacctgcagg tcgagcgtca gcggaccgtt gccgccgcgg ccgcggcggc
     3421 gcaagacgcg gaactcctgg ccgttgagca ggaagctgaa gacgacctcc atctcgctgg
     3481 cgttgagcgc catcaactga cggtcattgt tgacgcgggc tttaccccag agcgcccagg
     3541 taatacagtc gagcagggcg gacttccccg cgccgttctc gcccgagagg caggcgacgc
     3601 gcacgccgcg caggtcgatc
//
LOCUS       DASUBI010000477         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1309846, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000477 DASUBI010000000
VERSION     DASUBI010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309846"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFU72_09140"
     CDS             complement(<1..710)
                     /locus_tag="VFU72_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423692.1"
                     /translation="MLTRRTFVRGSLVAVAGGIAMPNIFARALQVAASDHVSDAGTRT
                     LIVIQLAGGNDGLNTVVPYQDSVYLQSRPTISVKAAEALPLDGRLALHPNLKELKGIW
                     DQGHLAIVESVGYDHPSLSHFQSMDIWQTADPTQGRRDGWLSKLVEGMVDSQGHPLGA
                     LALGAGLPPALCCPPIPPPVVDRADRYRLLSDPRHPELRSSRDEALQKLYASYQAPAP
                     YAALLEATSESAATSSTFL"
     gene            complement(724..>2248)
                     /locus_tag="VFU72_09145"
     CDS             complement(724..>2248)
                     /locus_tag="VFU72_09145"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEU5423693.1"
                     /translation="RRAMATSGTEAERHELSRREAVSGLALAGLGALLAACRVDHGHD
                     APGSSPAANGTPAPLVSVPGSPSASPEATVSAAAPLSQQARYAHLLRRAGFGAGPGKL
                     DQWLPLSWSVAVDRLVDYESIANDALDSRLAALKLDLTTALGIQRWWVLRMAFSARPL
                     EEKMTLFWHGLLTSSLTSAQPELMLDQNRFFRAHALDHYGDILKGVTRDPAMMRWLNT
                     AENKKGHANENYSRELMELFTLGPGNYSEQDVRESARAFTGLVTRKSGETVLVPAQHD
                     EGQKTFLGRSGNFGPDDIIDIILAQPAAAPFLARKLLGFFLSPDPTQASVDTIAGTLT
                     TSDFDIRQAVRALFNLPEFQDPASYRALVKSPAELIAGTLRRLDLDADQSPIPALMPE
                     MGQTLFAPPNVAGWPGGSAWLNSGTWLARLNFANALTTNRTAVQLDPAHWPGAEGGTA
                     QFVDTLADLLVDGNLDGSQRQVLLDFAGSAPADDTWFETQGRATVYLMLALPEYHLS"
BASE COUNT          331 a          746 c          801 g          370 t
ORIGIN      
        1 agaaaggtgg agctggtggc ggcgctctcg gaggtcgcct ccagcagcgc ggcgtagggc
       61 gcgggtgcct ggtagctggc gtagagcttc tgcagcgcct cgtcgcggct gctgcgcagc
      121 tcggggtggc gcggatcgga caggagacgg tagcggtcgg cgcgatcgac caccggcggt
      181 ggaatgggcg ggcagcagag cgcgggcggc agcccggctc cgagcgccag cgcgcccagc
      241 gggtgcccct ggctgtcgac catgccctcg accagcttcg agagccagcc gtcgcgccgc
      301 ccctgcgtcg ggtcggccgt ctgccagatg tccatagact ggaagtgcga caggctgggg
      361 tggtcgtagc cgacgctctc gacaatcgcc agatgcccct gatcccagat gcccttcagc
      421 tccttgagat tgggatgcag cgccagccgt ccgtcaagcg gcagcgcctc cgccgctttg
      481 acgctgatgg tcgggcgcga ctggaggtag acgctgtcct ggtaggggac gacggtattc
      541 aggccgtcgt tgcccccggc cagctgaatg acgatcaagg tgcgcgtgcc cgcgtcgctg
      601 acgtggtcgg aggcggccac ctgcaacgcg cgggcgaaga tgttcggcat ggcgatgccg
      661 ccggccacgg cgaccaacga gccccggacg aacgtgcggc gtgtcagcat aatccgtctc
      721 ccgtcagctc aggtgatact ccggcaacgc cagcatcagg tagaccgtgg cgcgcccctg
      781 cgtctcgaac caggtgtcat cggctggtgc gctgccggcg aagtccagca gcacctgacg
      841 ctggctcccg tcgaggttgc cgtcgaccag cagatcggcg agcgtgtcca cgaactgcgc
      901 cgtgccgccc tccgcgcccg gccaatgcgc ggggtcgagc tggacggccg tgcggttggt
      961 ggtcagcgcg ttggcgaaat tgagccgagc cagccaggtg ccgctgttga gccaagcgga
     1021 tccgcccggc caaccggcca cgttgggcgg cgcgaacagg gtctgcccca tctccggcat
     1081 cagcgctggg attggactct ggtcggcgtc cagatcgagc cggcgcagcg tgccggcgat
     1141 cagctcggcc gggctcttta ccagcgcgcg gtagcttgcg ggatcctgga actcgggcag
     1201 gttgaacagg gcgcgcacgg cttggcggat atcgaaatcc gaggtcgtca gggtgccggc
     1261 gatggtgtcg acgctggcct gcgtggggtc tggtgacagg aagaagccca gcagcttgcg
     1321 cgccaggaag ggcgccgccg ccggttgcgc caggatgatg tcgatgatgt cgtccggtcc
     1381 gaagttgccg ctgcggccga ggaaggtctt ctgcccctcg tcgtgctggg ccggcaccag
     1441 caccgtctca ccgctcttcc gcgtcaccag gccggtgaac gcgcgggcgc tctcgcggac
     1501 gtcctgctcg ctgtagttcc ctgggcccag cgtgaagagc tccattagct cgcgtgagta
     1561 gttctcgttg gcatgcccct ttttgttctc ggcggtgttg agccagcgca tcatggccgg
     1621 gtcgcgcgtc acacctttga gaatgtcgcc atagtggtcg agcgcgtggg cgcggaagaa
     1681 gcggttctga tcgagcatca gctcgggctg ggcgctcgtc agcgacgagg tcagcaggcc
     1741 gtgccagaag agcgtcatct tctcttccag cgggcgggcg ctgaacgcca tgcgcagcac
     1801 ccaccagcgc tgaatcccca gcgccgtcgt gagatcgagc ttgagcgccg ccaggcgcga
     1861 gtcgagcgcg tcgttggcga tggattcata gtccaccagc cggtcgaccg ccacgctcca
     1921 gctcaacggc agccactgat ccagcttacc gggtccggcg ccgaagcctg cacggcgcag
     1981 caaatgggcg tagcgcgcct gctggctgag cggagcggcc gcgctcacgg ttgcttcagg
     2041 cgacgcggag ggacttccag gcacgctgac cagcggcgcg ggcgttccgt tcgccgccgg
     2101 tgaacttccc ggcgcatcgt gtccatgatc gacgcgacag gctgccagca aggcgccgag
     2161 tccagcgagc gccagcccgc tgaccgcctc gcgccggctc agctcgtgcc gttccgcttc
     2221 ggtcccgctt gtcgccatcg ctcgcctc
//
LOCUS       DASUBI010000478         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_619226, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000478 DASUBI010000000
VERSION     DASUBI010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619226"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFU72_09150"
     CDS             <1..1190
                     /locus_tag="VFU72_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423694.1"
                     /translation="ALAYFGERDYADIFARLDRERDTDWKARFRQHPLYDAYVRQPLL
                     GHDLAGPDRDASLTLPPSPRQSWWHQFKTLTRRYVVVTASDRRNLLTLLLQAPLLGLL
                     ILLIAPPHSFDPSPAANHQQSITVALMIVLAITFMGAANAIREVVKELPIYRRERAVG
                     LSISAYIASKVAVLSVLTAAQALVLLLLAAMRQHGPTASAAGLPPALELYLDVVLTGI
                     AAMTVSLLISTAVTASDKAMTLLPLLLIPQIILSGGIISLEGKAGMSQLSDIVSANWG
                     FSAVASTIDLNFLDNPAHPNRAPSGEAANQSWARSRAVWLRDVTALLLLGGAAIAGAL
                     LLLQRRDPFVVRAGPLSAAVGDLLKRSGMQTGTSTFAREAMHRFVQNGLWLVLYGVLH
                     FLK"
     gene            1282..>2287
                     /locus_tag="VFU72_09155"
     CDS             1282..>2287
                     /locus_tag="VFU72_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5423695.1"
                     /translation="MTEIAGFTWWQTGIIYQIYPRSFQDSDGDGIGDLPGISCRLDYL
                     QWLGVDAIWLSPIFPSPMTDFGYDVSDYTAIHPLFGTLADFDRLLAEAHQRGLQVILD
                     YVPNHSSNQHPWFLESRAARDNPKRDWYIWRDPAPGGGPPNNWVSNFGGSAWEWDEPT
                     GQYYYHAYLPEQPDLNWRNPAVRRAMFDVLRFWLERGVDGFRVDALRQVIKDAEFRDN
                     PPNPAYTPEQGPYAALLPVYSTDRPEVHEVIRGLRAVMDAYPNRLMVGELYLPLERLV
                     AYYGEHGSGVHLPANFHLIQTPWRAADIAALVDEYEGLLPEGAWPNWVLGNHDRQRIA
                     S"
BASE COUNT          328 a          840 c          730 g          389 t
ORIGIN      
        1 aggcgctggc ctactttggc gagcgtgact acgccgacat cttcgcgcgt ctcgaccgcg
       61 agcgcgacac cgactggaag gcgcgcttcc gccagcatcc gctctacgac gcctacgtgc
      121 gccagccgtt gctcgggcac gacctcgccg gcccggatcg cgacgcgtcg ctgacgctgc
      181 cgccgtcgcc gcgccagagc tggtggcacc agttcaagac gctcacccgg cgctacgtcg
      241 tggtcactgc cagcgaccgg cgcaacctgc tgacgctctt gctccaggcc ccgctcctcg
      301 gcctgctgat cttgctgatc gcgccgccac actccttcga tccctcgccg gcggccaacc
      361 accagcagtc gatcacggtg gcgctgatga tcgtgctggc gatcaccttc atgggcgccg
      421 ctaacgccat ccgcgaggtg gtcaaggagc tgccgatcta ccgccgcgag cgcgccgtgg
      481 gtctctcgat ctcggcctac atcgcctcga aggtggccgt gctctcggtg ctgaccgcgg
      541 cccaggcgct ggtgctgctg ctgctcgcgg ccatgcgcca gcatgggccg accgcctcgg
      601 ccgccggttt gccgcccgcg ctggagctct acctcgacgt ggtgctcacc ggcatcgcgg
      661 ccatgacggt ctcgctgctg atctccaccg cggtcacggc ctcggacaag gcgatgacgc
      721 tcctgccgct cctcctgatc ccgcagatca ttctctccgg gggcatcatc tcactggagg
      781 gcaaggcggg gatgagccag ctcagcgaca tcgtctcggc caactggggc ttctcggcgg
      841 tggcttccac gatcgacctc aacttcctgg acaaccccgc ccaccccaac cgtgcgccga
      901 gcggtgaggc ggccaaccag agttgggcgc gctcgcgcgc ggtctggctg cgcgacgtca
      961 ccgcgctgct cctgctgggc ggcgcggcga tcgcgggtgc gctcctcctc ctgcagcggc
     1021 gcgatccttt cgtggtgcgc gccgggccgc tcagcgcggc ggtgggtgat ctgctcaagc
     1081 gctccggcat gcagacgggc accagcacct tcgcccgcga agcgatgcac cgcttcgtgc
     1141 agaatggcct ctggctggtc ctctacggcg tgctgcattt tctgaagtga tccctggggg
     1201 ctcccagggc ggcggcgcgg gcgacgccgg gctatcattc gctcctggag aaacgtcgtc
     1261 tcctggagag ggcgcaacac cgtgaccgag atcgccggat tcacgtggtg gcagacgggg
     1321 atcatctacc agatctaccc gcgctcgttt caggacagcg acggcgatgg tatcggtgac
     1381 ttgccgggta tcagctgccg cctcgactac ctccagtggc tcggcgtcga cgctatctgg
     1441 ctctcgccga tcttcccctc gccgatgacc gacttcggct acgatgtcag cgattacacg
     1501 gcgatccacc cgctgttcgg cacgctcgcc gatttcgacc gcctgctggc cgaggcgcac
     1561 cagcgcgggc tgcaggtcat cctcgactac gtgcccaacc actcctcgaa tcagcacccc
     1621 tggttcctgg agtcgcgcgc ggcgcgcgat aacccgaagc gcgactggta catctggcgc
     1681 gacccggccc ccggcggcgg cccgcccaac aactgggtca gcaacttcgg cggcagcgcc
     1741 tgggagtggg acgagccgac cgggcagtac tactaccacg cctatctacc agagcagcct
     1801 gacctcaact ggcgcaaccc ggcggtgcgc cgggcgatgt tcgacgtgct gcgcttctgg
     1861 cttgagcggg gtgtcgacgg cttccgcgtc gatgccttgc gccaggtcat caaggacgct
     1921 gagtttcgcg acaacccgcc caatccagcc tacacgcccg agcagggtcc gtacgccgcg
     1981 ctcttgccgg tctactcgac cgaccggccc gaggtccacg aggtcattcg cgggctgcgg
     2041 gcggtcatgg atgcctatcc gaaccggctg atggtcggcg agctctacct gcccctggag
     2101 cggctcgtcg cctactacgg cgagcacggc agcggcgtcc acctgccggc caactttcac
     2161 ctgatccaga ccccctggcg ggcggccgac atcgccgcgc tggtcgacga gtatgagggg
     2221 ctgctgccgg agggcgcctg gcccaactgg gtgctcggca accacgaccg ccagcgcatc
     2281 gccagcc
//
LOCUS       DASUBI010000479         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_721783, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000479 DASUBI010000000
VERSION     DASUBI010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721783"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFU72_09160"
     CDS             complement(<1..427)
                     /locus_tag="VFU72_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5423696.1"
                     /translation="MRYLHVIQRYFPFQGGSERYFQALSERLAAGGDEVQVVTSDAWD
                     LEHFWDPSRSTVQDGSLEHNGVAIRRVPVAHLPLPSLSHRAIRRLMADSCRLPLPGRR
                     ALLRLGSRFGPWLPALPAALDEAGTVDLVHSANIAFESVI"
     gene            complement(438..1064)
                     /locus_tag="VFU72_09165"
     CDS             complement(438..1064)
                     /locus_tag="VFU72_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423697.1"
                     /translation="MARKARQPSYAVPRKRSRWLPLFAAIGIALMVLGLLVVLIPDGG
                     SSNAGDSSSVLPPPTPGAAEAQMRARLEKDPNDVNAMSVLADILANTGRVDEAVPYYE
                     KVIQQKPSDETLRVAFGSALMHANYNGDAEQELLKAHDLAPSDPQPLYLLGQLYEGWQ
                     PERLDDAKKMYQAVLDAAPDSSYAARAKDRLSALSSTPAPPGTPSGTP"
     gene            1229..1313
                     /locus_tag="VFU72_09170"
     tRNA            1229..1313
                     /locus_tag="VFU72_09170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:gag)
     gene            1331..1403
                     /locus_tag="VFU72_09175"
     tRNA            1331..1403
                     /locus_tag="VFU72_09175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1364..1366,aa:Val,seq:tac)
     gene            1500..1575
                     /locus_tag="VFU72_09180"
     tRNA            1500..1575
                     /locus_tag="VFU72_09180"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1533..1535,aa:Phe,seq:gaa)
     gene            complement(1725..2219)
                     /locus_tag="VFU72_09185"
     CDS             complement(1725..2219)
                     /locus_tag="VFU72_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEU5423698.1"
                     /translation="MMTRACRGRTRLFVLSLLLTLCGLTLAAPASAAGGSGQLAVAPA
                     TVPPVDLAVNQITMNVGAVCVGDVVIFRAQVTNLGSDPSRWFSYQWFVDGRPTFPLIH
                     RSLKGGQLATVWFQWQQFSAGSHNVRLVLDPRHRIAEPDEVNNWFAVAFRVYDTSNPV
                     CGAP"
     regulatory      2517..2657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2811..5039
                     /locus_tag="VFU72_09190"
     CDS             2811..5039
                     /locus_tag="VFU72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5423699.1"
                     /translation="MTDLPQSRMLAARYHGPAEGFALEEQPLPLPRAGEVLLKVLAAG
                     ICHTELHLLEGDLNPGVVPLTPGHEIAGEVAAVADDVHDWSPGQRAVVYYYAACGECR
                     WCRSGQEQLCPNVGRQIGFTADGGYAQYVVVPARSLVPIPGSLHPAEACTLGCSAATA
                     LHAVRAIAELRLGETAVVYGVGAVGYALVQIAKLAGARVVAIGRTPAKLELARELGAD
                     VVVNAAERDPVAAVQAATGGQGADAVFDLVASRQTMDDGVRMLARRGRLVLVGYGDEP
                     LVVNPLLLVLRETQIRAAVGNSYRELAEVVELAVHGQYRSLVARVEPLEQVNDLLGAL
                     SRGALLGRAVLAPNGPLPPLPEHQARPEIVLQDSQTKRHESPPLEQDLLAVVARGLEQ
                     PLPDAEFDALARRVFAYQYARNEPYRRYCDLRGVSPDSLTDWCAIPAVPIAAFKETTL
                     VCEPVEAAEAVFMSSGTTRADQRSRHYHPNLRVYDANAAANWRAHLLPDLADGQRMRL
                     LILNPPPDQMPNSSLAHYLDLMLRRFGAPESAFYVGPTGLDLAGLDDALRESEAAGDP
                     VCVLGATFAFVHLLDRAVEDRVTFRLPEGSRVMDTGGVKGKSREIGRSEFLQEVSSLF
                     AIPRHHIVNMYGLTELSTQFLDASLRDHVAGRRLPRYKTVPPWARTRVLDPETLEEVA
                     PGEIGLLCHLDLANRASVSAILTEDLGYELGDGFEIVGRVRGSEARGCSIALDELLQA
                     VR"
     gene            5042..>6081
                     /locus_tag="VFU72_09195"
     CDS             5042..>6081
                     /locus_tag="VFU72_09195"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEU5423700.1"
                     /translation="MERFDAFHLPGLLLPSDQPFRELAFGEGASRVVARAPVLSPEQI
                     RDLSLAVRAARERRLDRLPVLVIANLIARAINRWLDPYSPYLRDCERLLPVVTGYPVE
                     AIRKGLPAFLGGYREEHLRRLLDDEFGDPTVLDGFRPRLAASGLTRAFGPRLAAHIFA
                     GNVPGLPAQSLTCTLLVKAASIGKSASEEPCFPVLFARSLAEVDPELGAALAVTWWPG
                     GERSVEEALFDEAEAIIAYGSEAAIESVRAVLPPGRRFIPYGHKISIGLIGREALSRD
                     VIDGTVAAAAYDTAKYDQQGCLSPHLFYLERGGEISPEDFAERLAAELARVQGIIPRG
                     RLNRDEATAIAEL"
BASE COUNT          957 a         2163 c         2007 g          954 t
ORIGIN      
        1 cgatcaccga ctcgaaggcg atgttggccg agtgcaccag atcgaccgtg ccggcctcat
       61 ccagcgccgc cggcagcgcc ggcaaccagg ggccaaagcg cgagccgagc cgcagcagcg
      121 cccgccgccc ggggagcggc agacggcacg agtccgccat cagccgccgg atcgcccggt
      181 gcgagagact gggcagcggt aggtgtgcca ccggcacgcg ccggatcgcc accccattgt
      241 gctccaacga tccatcctgc acggtactcc ggctcggatc ccaaaaatgc tccagatccc
      301 aggcgtcgct ggtgacgacc tggacctcat cgccgccggc cgccagccgc tccgagagcg
      361 cctggaagta gcgctcggag ccgccctgga aagggaaata gcgctggata acgtgcaggt
      421 agcgcatcga tcgcagccta cggcgtgcca gatggcgtac cgggcggagc cggcgtgctg
      481 ctcaaggcgc tcaagcgatc tttggcgcgt gcggcatagc tcgagtcagg cgcggcatcc
      541 agcaccgcct ggtacatctt cttggcgtcg tcgagccgct ccggctgcca gccctcatag
      601 agttggccca ggaggtagag cggctgcgga tcggacggtg ccagatcgtg cgccttcagc
      661 agctcctgct cggcgtcgcc gttgtagttg gcgtgcatca gcgcgctgcc gaaggccacc
      721 cgcagcgtct cgtcgctggg cttctgctgg attaccttct cgtagtaggg cacggcctcg
      781 tcgacacgac cggtgttcgc caggatatcg gccagcaccg acatcgcgtt gacgtcgttg
      841 gggtccttct ccaagcgggc acgcatctgc gcctcggccg cgccgggtgt cggtggcggc
      901 aggaccgagg aggagtcgcc agcatttgat gaacccccat cggggatcag caccaccagc
      961 agaccaagga ccatcaaggc gatgccgatg gcggcgaaga gcggcagcca gcgggagcgc
     1021 ttgcgcggaa cggcatagct cggttggcgc gccttcctag ccacgagtcg ctcagctcca
     1081 atcaccctca ccgggcgctg cggcacccgt tcgcaactgc cccgatcgaa ggatacgcca
     1141 ggcgccgtgc gtgggcgaaa tgcttgaccg gtcgcggccc cacgggtata ctttcgtgtg
     1201 cctgtcaatc aggttcagca ggcagcatgc cgaggtggcg gaataggcag acgcgccagc
     1261 ttgaggggct ggtgcccgtt tagggcgtgg gggttcaagt cccttcctcg gcacccaccg
     1321 gcgtcgcgtc gggcgattag ctcagttgga agagcgccac gtttacaccg tggatgtcag
     1381 aggttcgagt cctctatcgc ccacgagtca cggagaggcc cgatcgcctt cggacgtcgc
     1441 atcgagcccg tatcattccc cttcaatcgc gcacgtcgtc gggcaacaca agtcaatacg
     1501 gctgggtagc tcagttggta gagcactgca ctgaaaatgc aggtgtcccc agttcgaatc
     1561 tgggcccagc caccagccca cccatcaccc gcaaatctcg tcgccagcca tcggttccat
     1621 tctcccggtc accggaaacg tcgcccgtcc gtcccacgac acgcgaaact ggacctgagc
     1681 ggaacctgct ccaggccagt cgtcggctcc ctctggggag cggcttacgg cgcgccgcag
     1741 accggattcg aggtgtcgta gacgcgaaag gcgacggcga accagttgtt gacctcgtcg
     1801 ggctcggcta tgcggtggcg tgggtcgagc accagtcgca cgttgtggct tccggcactg
     1861 aattgctgcc actggaacca gacggtcgcc agttgaccac ccttcaggct ccgatggatc
     1921 agcgggaacg tcggcctgcc gtccacgaac cactggtagc taaaccaccg gctggggtcg
     1981 ctgccgagat tggttacctg agcacggaag atcaccacat ccccgacgca gaccgcgccg
     2041 acgttcatgg tgatctggtt caccgccagg tcgacgggcg gaactgtcgc cggcgccacc
     2101 gccaactgac cggaaccacc cgcggcactc gcgggggcgg ccagggtgag cccgcagagc
     2161 gtcagcagga gactgagcac gaacagccgc gtgcggcccc ggcaggcgcg cgtcatcatg
     2221 ttccctcccc cagttacagc gaattccgtc aaccaagacc acggcacaga cgggcgttcc
     2281 cgcggccgac catggaccgt gaccagaaac cagaaactgc tggacgggcg cgaccgcgat
     2341 ccaccagtga gacacgcgcg ctcgcaccgt acatccagat cggggcggtc agcgccgcac
     2401 aaacaagctg agtcaaccta atcgcagtct aattgggtac gtactaggcg tcaatacaca
     2461 ggggcaattg acggcgcccg cgcgccgcac tacaatccga cggacaagcg aatagcaggg
     2521 accgctgggg gagcgagcag ctgagaggcg gggtcaggcc caccaacccc ttgaacctga
     2581 tctcgggaag ccgcgcggcg agcgatgagc tcgggcgtcg cggaagacga gcgtagggaa
     2641 tgcggcgttc cgatccgatg ccgtcgcatc ggcgtgtcgc cgcgcctgag acggcgtttt
     2701 tttgtgccgg cgcaggcgcg tgttgcccgt atctccctcc tcccctttcc gactccgcat
     2761 cacttcccct cctgtcgcca atgggcaggt gcccagggac ggccgaccac atgaccgact
     2821 tgccgcaatc gaggatgctc gccgcgcgct accacggtcc ggcggaagga tttgcgctcg
     2881 aagagcagcc gctgccgctg ccgcgcgccg gtgaggtgtt gctcaaggtg ctggccgccg
     2941 gcatctgcca caccgagctg cacctgctgg agggtgatct caacccaggc gtggtgcccc
     3001 tgacccccgg ccacgagatc gccggggagg tcgccgccgt cgcggatgat gtccacgact
     3061 ggtcgccggg tcagcgcgcg gtcgtctact actacgccgc ctgcggcgag tgccgctggt
     3121 gccgctcggg ccaggagcag ctctgcccca acgtcggtcg ccagatcggt tttaccgccg
     3181 atggcggcta cgcgcagtac gtcgtcgtcc cggcgcgctc gctggtgccg atacccggct
     3241 cgctccaccc ggctgaggcc tgcaccctcg gctgcagcgc cgccacggcg ctgcacgcgg
     3301 tgcgcgccat cgcggagctg cgcctgggcg agaccgccgt ggtctacggg gtcggcgcgg
     3361 tcggctacgc cctggtgcag atcgccaagc tggccggggc gcgggtcgtc gcgatcgggc
     3421 gcacgccggc caagctggag ctggcacgcg agctgggcgc cgacgtcgtc gtcaacgccg
     3481 ccgagcggga tccggtcgcg gcggtgcagg cggcgaccgg gggccagggc gccgacgccg
     3541 tcttcgactt ggtcgccagc cggcaaacga tggacgacgg cgtgcgcatg ctcgcccggc
     3601 gcgggcggct ggtgctggtc ggctacggcg acgaaccgct ggtggtcaac ccgctgctgc
     3661 tagtgctgcg cgagacccag atccgcgcgg cggtcggcaa cagttaccgc gagctggccg
     3721 aggtggtcga gctggccgtc cacggccagt atcgctcgct ggtggcccgc gtcgagccac
     3781 tggagcaggt caacgacctc ctcggcgcgc tcagccgcgg cgcgctactc ggccgcgcgg
     3841 tgctggcgcc caacgggccg ctgccgccgc tccccgagca tcaggcgcgt ccagagatcg
     3901 tcctgcagga cagtcaaacg aagaggcatg agtcaccgcc gctggagcag gatctgctgg
     3961 cggtcgtcgc ccgcggtctc gagcagccgc tgcccgacgc tgagttcgac gcgctggcgc
     4021 gccgggtctt cgcctatcaa tacgcgcgca acgagcccta ccgccgctac tgcgacctgc
     4081 gcggcgtctc accagattcg ctgacggatt ggtgcgccat acctgccgtg ccgatcgcgg
     4141 ccttcaagga gaccacgctg gtctgcgagc cggtcgaggc ggccgaggcg gtttttatgt
     4201 cgagcggcac cacccgcgca gaccagcgca gccgtcacta ccaccccaac ctgcgcgtct
     4261 acgacgccaa tgccgccgct aattggcgcg cgcatctctt gcccgatctg gcagacggcc
     4321 agcgcatgcg gctgctgatc ctcaatccgc cacccgacca gatgcccaac agcagcctgg
     4381 ctcactacct cgacctgatg ctgcggcgct tcggtgcacc ggagagtgcc ttctacgttg
     4441 gccctaccgg tctcgacctg gctggcctgg atgacgccct gcgtgagtcc gaagcggctg
     4501 gcgaccctgt ctgcgtcctg ggcgccacct tcgccttcgt ccacctgctc gaccgcgccg
     4561 tcgaagaccg cgtcacgttc cgcttgcccg agggtagccg ggtgatggac accggcggcg
     4621 tcaagggcaa gagccgcgag atcggccgca gcgaatttct gcaggaggtc tcatccctct
     4681 tcgccattcc caggcaccac atcgtcaaca tgtacggcct gaccgagctc tcgacccagt
     4741 tcctcgatgc ctccttgcgc gaccacgtcg ccggtcggcg cctgccacgc tacaagacag
     4801 tgccaccctg ggctcgtacg cgcgtgctcg atcccgaaac gctggaggag gtcgcgccgg
     4861 gtgagatcgg cctgctttgc cacctcgacc tggccaaccg cgcctcggtc agcgccatcc
     4921 tgaccgaaga cctcggctac gaactgggcg atgggttcga gatcgtcggg cgcgtacgcg
     4981 gctccgaagc gcgcggctgc tcgatcgcgc tcgacgagct gctgcaggcg gtgcgctagc
     5041 catggagcgc ttcgacgcct tccatctccc cggcctgctg ctgcccagcg accagccctt
     5101 ccgcgagttg gcctttggcg aaggcgcgag ccgcgtcgtc gcgcgcgcgc cggtgctctc
     5161 gccggagcag atccgcgacc tgtcgcttgc cgtgcgcgcg gcgcgtgagc ggcgcttgga
     5221 ccggctgccg gtgctggtga tcgccaactt gatcgcccgc gcgatcaacc gctggctcga
     5281 cccctactcg ccctacctgc gcgactgcga gcgcctcttg ccggtcgtca ccggctatcc
     5341 cgtggaggcg atccgcaagg ggctgccggc cttcctcggc ggctaccgcg aggagcacct
     5401 gcgccggctg ctcgacgacg aattcggtga cccaaccgtg ctcgacggat tccgcccgcg
     5461 cctcgccgcc tccggtctga cgcgcgcctt cggcccccgc ctcgccgcgc acatcttcgc
     5521 cggcaacgtg cccggcctgc cggcccagag tctgacctgc acgctgctgg tcaaggctgc
     5581 cagcatcggc aagtcggcct ccgaggagcc gtgcttcccg gtgctcttcg cccgctcgct
     5641 ggccgaggtc gaccccgagc tgggcgcggc gctggcggtc acctggtggc ccggcggcga
     5701 gcggtcggtc gaggaggcgc ttttcgacga ggccgaggcg atcatcgcct acggcagcga
     5761 ggccgccatc gagtccgtgc gcgcggtgct gccgccgggc cggcgcttca tcccctatgg
     5821 ccacaagatc agcatcggcc tgatcggccg cgaggcgctc tcccgtgacg tgatcgacgg
     5881 caccgtcgcc gcggcggcct acgacaccgc caagtacgac cagcagggct gcctcagccc
     5941 gcatctcttc tacctggagc gcggcggcga gatctcccct gaggacttcg ccgagcggct
     6001 ggccgccgag ctagcgcgtg tgcagggcat catcccgcgc ggccgactca accgcgacga
     6061 ggctaccgcc atcgccgaac t
//
LOCUS       DASUBI010000480         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_204787, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000480 DASUBI010000000
VERSION     DASUBI010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204787"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..834
                     /locus_tag="VFU72_09200"
     CDS             148..834
                     /locus_tag="VFU72_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5423701.1"
                     /translation="MSERQSSPAESPLVHLIRPPRQAAEGPRPALLMLHGRGADELDL
                     FGLADALDPRLLVISARAPFPLGPGYHWYELVQIGEPDNASFFQSLGLLERFVEDSLQ
                     RYQIDPRRLYLLGFSQGAMMAGALTLSHPGWIAGSILLSGYLPLDTGLPVDREHLAGE
                     PFFVAHGTRDTVVPIQLGRRTEEFLSQVDADLTYREYSIAHQISLEEMEDFGAWLSAL
                     LDEQESPSQP"
     gene            831..1361
                     /locus_tag="VFU72_09205"
     CDS             831..1361
                     /locus_tag="VFU72_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423702.1"
                     /translation="MSLAGLAIRPLAAGDIPEIAAAFAALGWNKPAAQYQRYLREQER
                     GERAALLARLDGRFAGYLTVVWRSGYPPFRAAGIPEIVDFNVLPDLRRRGIGSRLLDA
                     AEELIAARWPLAASLAGIGVGLDPDYGAAQRLYVERGYLPDGRGLAWRGQPVAYGQSV
                     TVDDDLALYFTRELRR"
     gene            1388..2341
                     /locus_tag="VFU72_09210"
     CDS             1388..2341
                     /locus_tag="VFU72_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEU5423703.1"
                     /translation="MQTPGIHHVTSIAAEPQQNLDFYGPLLGLRPVKLTVNFDLPQTY
                     HLYYGDLTGSPGTILTMFPWPKGRRGRPGAGQIAATGFAIAPESLGYWRDRLREQDVE
                     VEGPLPRFDEEVLRFVDHDGLINELVAREGVRPAHAWEGGPVPVEHAIAGFSGVTLHS
                     RQPEATARVLTELLGFTPAGEEAGRQRFLADRQALGAVVDLEPAPAVDGTEGTGTVHH
                     VAFRTRDDAEQAAWRERIAAAGLAVTPVRDRQYFRSIYFREPGGVLFEIATDAPGFAT
                     DETVEQLGSHLKLPPWLEERRAAIERGLLPVRLPSGARVPA"
     gene            complement(2349..>2786)
                     /locus_tag="VFU72_09215"
     CDS             complement(2349..>2786)
                     /locus_tag="VFU72_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5423704.1"
                     /translation="FSAGAQPEEERRASRDARLRALATMTPAEMAEARVDAVLAPDPP
                     VALRREVVDMLAAIRPSGYAAAANAIYGADERAALPRIDLPVLVVAGDQDAVVPLERV
                     EQLAAAIPGARLAVFPGSGHLTNQEQPRRFDQVLGDFLAGVDA"
BASE COUNT          370 a         1044 c          982 g          390 t
ORIGIN      
        1 tcgctgccgg atagtagcac aattgttctg tacagccaat ccgggccttc agatgcacgc
       61 ggcgggaatg cgtatgcgtc ctacggtatg ctggttgcgg cacagtcgtg cacggggaat
      121 gcccgcgagc gaacagaggg agtggtcatg agcgagcgcc aatcatcgcc ggccgagtcg
      181 ccgctggtcc acctgatccg cccgccgcgc caggccgccg agggtccgcg cccggcgctc
      241 ttgatgctgc acggccgggg cgccgacgag ctggacctct tcgggctggc cgacgcgctc
      301 gacccgcgcc tgctggtcat cagcgcccgc gcgcccttcc cgctcggccc cggctaccac
      361 tggtacgagc tggtgcagat cggcgagccg gacaacgcca gcttcttcca gtcgctcggg
      421 ctcttggagc gcttcgtcga ggacagcctg cagcgctacc agatcgatcc gcgccgcctc
      481 tacctgctcg gcttcagcca gggcgccatg atggccggag cgctgacgct cagccaccct
      541 ggctggatcg ccggctcgat cctgctcagc ggctacctgc cgctcgacac cgggctgccg
      601 gtcgatcgcg agcacctcgc cggcgagccc ttcttcgtcg cgcacggcac gcgcgatacg
      661 gtcgtgccga tccagctcgg gcgccgcacc gaggaattct tgagccaggt cgatgccgac
      721 ctgacctatc gcgagtactc gatcgcccac cagatcagtc tggaggagat ggaggacttc
      781 ggcgcctggc tcagcgcgct gctcgacgag caggagagcc cgagccagcc gtgagccttg
      841 ctggcctggc gatccggccg ctcgcggccg gcgacatccc cgagatcgcg gcggcattcg
      901 ccgcgctcgg ctggaataag ccggcggcgc agtatcagcg ctacctgcgt gagcaagagc
      961 ggggcgagcg cgcggcgctg ctggcccggc tggacgggcg cttcgccggc tacctgaccg
     1021 tcgtctggcg ctcgggctac ccgccctttc gcgcggcggg cattcccgag atcgtcgact
     1081 tcaacgtgct gcccgatctg cgccgccgcg gcatcggctc gcgcctgctc gacgccgccg
     1141 aggagctgat cgccgcgcgt tggccgctgg ccgcgtcgct ggccggcatc ggcgtcggcc
     1201 tggaccccga ctacggcgcc gcccagcggc tctacgtcga gcgcggctac ctgccggacg
     1261 gccgcgggct ggcctggcgc gggcagccgg tcgcctacgg ccagtccgtc accgtcgacg
     1321 acgacctggc gctctacttc acgcgagaac tccgtcgctg acagcgcgat cctcgaaagg
     1381 agtcccgatg caaacacccg gcatccacca cgtcacctcg atcgcggccg agccgcagca
     1441 gaacctcgac ttctacgggc cgctgctcgg gctgcgcccg gtcaagctga cggtcaactt
     1501 cgacctgccc cagacctacc acctctacta cggcgacctc accggcagcc cgggaaccat
     1561 tctcaccatg ttcccctggc ccaaggggcg acgtgggcgg ccgggcgccg gccagatcgc
     1621 ggccaccggc ttcgccatcg cgccggagtc gctcggctac tggcgcgacc ggctgcgcga
     1681 gcaggatgtc gaggtggagg ggccgttgcc gcgcttcgac gaggaggtgc tgcgcttcgt
     1741 cgaccacgac gggctgatca acgagctggt ggcgcgcgag ggagtacgac cggcgcacgc
     1801 ctgggagggt gggccggtgc cggtcgagca cgccatcgcc ggcttcagcg gggtcacgct
     1861 gcactcgcgc cagccggagg cgaccgcacg cgtgctgacc gagctgctcg gcttcacccc
     1921 ggccggcgag gaggcggggc ggcagcgctt cttggccgac cgccaggcgc tcggcgcggt
     1981 ggtcgatctg gagccggcgc cggctgtcga cggcaccgag ggcaccggca cggtgcacca
     2041 cgtcgccttc cgcacccgcg acgacgccga gcaggcggca tggcgcgagc gcatcgccgc
     2101 cgccgggctc gccgtcacgc cggtgcgcga ccgccagtac ttccgctcga tctacttccg
     2161 cgagccgggc ggcgtccttt tcgagatcgc gaccgatgcg cccggcttcg ccaccgacga
     2221 gacggtggag cagctcggca gccacctgaa gctgccgccc tggctggagg agcgccgcgc
     2281 cgccattgag cgcggcctgc tgccggtgcg cctcccctcg ggcgcgcggg tgccggcctg
     2341 agcgcgactc aggcgtcgac ccccgccagg aagtcgccga ggacctggtc gaagcgtcgc
     2401 ggctgctcct ggttggtcag gtggccggag ccggggaaga cggcgagccg cgcgccgggg
     2461 atggccgccg ccagctgctc gacgcgctcc agcggcacca cggcgtcctg atcgccggcc
     2521 acgaccagca cgggcaggtc gatgcgcggc agcgcggcgc gctcgtcggc gccgtagatc
     2581 gcgttggccg cggcggcgta gccgctgggg cggatggccg ccagcatgtc gaccacctca
     2641 cgccgcagcg ccaccggcgg atcgggcgcc agcacggcat cgacgcgcgc ctcggccatc
     2701 tcggcgggcg tcatcgtcgc cagcgcccgc agccgggcgt cgcggctcgc ccgccgctcc
     2761 tcctccggct gcgcgccggc gctgaa
//
LOCUS       DASUBI010000481         6860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_269795, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000481 DASUBI010000000
VERSION     DASUBI010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269795"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFU72_09220"
     CDS             complement(<1..425)
                     /locus_tag="VFU72_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423705.1"
                     /translation="MSGANANVSVNINGNSAGAKQAIQDVLEALRQLNNQAGAGRSMM
                     SQYGFSWTTLGQSILGSLGPMGSWLVVAGSIVSLATPFIDAFQNAADVVVQFAEHLGM
                     DLVQAAQAAAQEIENLTQQVMDLNVQTQKNLFTWSYLQGG"
     gene            complement(422..811)
                     /locus_tag="VFU72_09225"
     CDS             complement(422..811)
                     /locus_tag="VFU72_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423706.1"
                     /translation="MPISRPGTDPEAEALAAAYRREAGLAAEPGDDDDDDDEGGRDDQ
                     GEGEGKRAGRVDVLEVLDAMSERYHEHRREMLERWSWPFFCAQWARLLDYARREKRKK
                     QRRARQQRQQATAAELEQRLTTGVSEG"
     gene            complement(814..1311)
                     /locus_tag="VFU72_09230"
     CDS             complement(814..1311)
                     /locus_tag="VFU72_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423707.1"
                     /translation="MKSGLNFDEIALKAKTELIEKHGREWRLRGDVPSNVMIERFLLK
                     DLGERFKGVIDAMKVEDPDGTRYTPDELRRRLEAEVSGYQADVLAICTAIFRHTLPET
                     KAEEIAVLFNPDEQKAIVDHFFTLRIGSSSQPESAGAASSDAPPAEPNRAARRSRAKT
                     AKAKH"
     gene            complement(1392..2600)
                     /locus_tag="VFU72_09235"
     CDS             complement(1392..2600)
                     /locus_tag="VFU72_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423708.1"
                     /translation="MNWGTAVAPTWFHKWLDGTKEMPQVRLQNEMEGDVTQTETLTYK
                     QAQFRQFTIIEYARPVIAGYALQSLLGTNSDTLTAAAKSSTLSAPITAGATSFTSVGD
                     LGNVGPSQAVGFSTAYGDTSYEVATVDLTTRTGAGPWVYTLAASGTFKKAHLASDPIA
                     FPSTHAFSESAVFTYDPYSYEFGYGVSGGTPNRVLRVKDAVCYGLRIRADAGKPVIFE
                     HDWFGAMASVQAAFTAVVQDSGPPLLFYQGAGAWKLNALTTGNAATIKQLSVELKRNT
                     AAEDLINENLTPAYFLPGALTAGGSLTVDFSSWQEYDQAYYGKAAAQTADTDSFIVGT
                     EALETTITADGVNSFKVSLPTINFAAATLEPKLDSKPLDQQIQFTAIRPPAQAKLLTI
                     TVTNNQNTAY"
     gene            complement(2754..3266)
                     /locus_tag="VFU72_09240"
     CDS             complement(2754..3266)
                     /locus_tag="VFU72_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423709.1"
                     /translation="MPTAVKGGEAAATALANTLATYINAEVDAIAAAWGDSATVPLPH
                     VAAANIYTLRRAQLPAEGVALVVSVLGGEQTLNHATEWGQLAHHLEVAVVCQSDDLYV
                     LEAQVDRYLTAVWQVVMKHPGLDATVATVVGCDPVRYGRSETYPHPKLPLMVLGGAWE
                     VVVYVEQVTS"
     gene            complement(3277..3771)
                     /locus_tag="VFU72_09245"
     CDS             complement(3277..3771)
                     /locus_tag="VFU72_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423710.1"
                     /translation="MSGPAGFFELHVSVDGVEEINRRFVALHQRVSDLSPAFAAIGDE
                     LRADFGLNMATEGGRYGGWAPLAPSTIKEKARRWPGAPMEWRTGMLGRSLSQLGAPGN
                     VSVVSAMSAMFGTSIPYATFQHFGTRRMPARKLVGLSWYTKSKIVRAVADYIRQASGG
                     MATP"
     gene            complement(3793..3942)
                     /locus_tag="VFU72_09250"
     CDS             complement(3793..3942)
                     /locus_tag="VFU72_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423711.1"
                     /translation="MSYHGTHRKSVKPKKPKTYRGKPRRPSSHLLAAAKRDPWQHKGQ
                     AKSGV"
     gene            complement(3979..4545)
                     /locus_tag="VFU72_09255"
     CDS             complement(3979..4545)
                     /locus_tag="VFU72_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423712.1"
                     /translation="MAADASPPVITDASVTLTFGAAGQYYGYATIADVKFEFPNAASF
                     STLTNSVVGQEITYAALELQKMLDHWYVMPYTGSSFNVTTTLREMNAKLATARIIKRY
                     FSGSEPDFSPAAEAMRQDVELLVTDIENGKLRFEDPWGDATARAMLPVYDLAKGATIY
                     PNPSIADTSAATPIFTIARPTFKRDDQF"
     gene            complement(4572..4898)
                     /locus_tag="VFU72_09260"
     CDS             complement(4572..4898)
                     /locus_tag="VFU72_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423713.1"
                     /translation="MAKFSGIAQGRIEHGEGVDANGTPVVHEYRHGETFETDDEALWN
                     ALRSANAVKLPGEIALSEEQAAAALNAAQQQAAELEMARNRIAELEALVRSQSENKAQ
                     GKKLPE"
     gene            complement(5007..5996)
                     /locus_tag="VFU72_09265"
     CDS             complement(5007..5996)
                     /locus_tag="VFU72_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423714.1"
                     /translation="MATQASPNFTVGNVHVSAPLTQLSIGYHPMGMIAEQIFPVIRVN
                     HENDLYYVWDQAQAFRLERGDGYDDMRADGTEARTESYGATLKSYQAYEFARRTTVTD
                     RERGNADSVLQLETSKIRRTQDKILLNQELRIGKILTTTANYDSTNFVTLAGTSQWNN
                     ASFASQNTATQSVIKKNIDDGKEGIRSATGGLEANTIVVPAAVARVMARDVGIVEQVK
                     YTHPDLLVGGYLPQTLWGMKVLMPTAIYSTSVEGEVPTYTDVWGKNVILAYVNPNPGV
                     DSITAGSIFRARDWQVKTWRDEAIDTTFYQASLVQTEVLVAKQAAYLIQSAIA"
     gene            complement(6079..6561)
                     /locus_tag="VFU72_09270"
     CDS             complement(6079..6561)
                     /locus_tag="VFU72_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423715.1"
                     /translation="MATSNRLFTLPFQASSNVTAVTLTGNPPAYSSGGLVQFTAVVFD
                     TATGNPGDIKGATVNTAPILGVLQNSTYPAGNSTAGAIGPGDSADIMVYGVTKMVAGA
                     AITYGALVSVDSSGRAVTAPAAGVSQVYAIGIAKGNATALGDLVEVILLPGVATQVNA
                     "
     gene            complement(6630..>6860)
                     /locus_tag="VFU72_09275"
     CDS             complement(6630..>6860)
                     /locus_tag="VFU72_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423716.1"
                     /translation="TFGASFDQEARRTIRQGGGADARGVARVEDELRIVETAEQLALS
                     DKKIERGQTLTALALSDRERYMDLAAKEVGYK"
BASE COUNT          931 a         2628 c         1994 g         1307 t
ORIGIN      
        1 ccgccttgca ggtagctcca ggtgaacagg ttcttctgcg tctggacgtt gaggtccatc
       61 acctgctgcg tcaggttctc gatctcctgc gccgccgcct gcgccgcctg caccagatcc
      121 atgcccaggt gctcggcgaa ctggaccacc acgtccgcag cattctggaa ggcgtcgatg
      181 aagggcgtgg cgagcgaaac gatgctaccg gccaccacca accacgaccc catcggcccc
      241 agactgccga ggatggactg gccgagcgtc gtccaactga aaccgtactg gctcatcata
      301 gagcggccag cgcccgcctg gttgttcagc tgccgcagtg cctccaggac atcctggatg
      361 gcctgcttcg cccccgcgct gttgccgttg atattgacgc tgacgttcgc gttcgcgccg
      421 ctcatccttc agataccccc gtcgtcagac gctgctctag ctccgccgcc gtcgcctgct
      481 gccgctgctg ccgcgcccgc cgctgcttct tgcgcttctc gcgccgcgcg tagtccagca
      541 gccgcgccca ctgcgcgcag aagaacggcc agctccaccg ctccagcatc tcgcgccggt
      601 gctcgtggta gcgctcgctc atcgcgtcca gcacctccag cacgtccacc cggcccgcgc
      661 gcttgccctc gccctcgccc tggtcgtccc tccccccctc gtcgtcgtcg tcgtcgtcgt
      721 cgcctggctc cgctgccagc cccgcctcac gccggtacgc cgcggccagc gcctccgcct
      781 ccgggtccgt tcccggccgc gagattggca ccgtcagtgc ttcgccttgg ccgtcttggc
      841 tcgactccgc cgcgccgccc gattcggctc cgccggcgga gcgtccgaac tcgcggcccc
      901 agcgctctcc ggctgcgacg acgagccgat acgcagggta aaaaaatggt ccacgatcgc
      961 cttctgctcg tcagggttga acagcaccgc gatctcctcg gccttcgtct ccggcagcgt
     1021 gtgccggaag atggccgtgc agatcgccag cacgtcggcc tgatagcccg acacctccgc
     1081 ctctagccgc cggcgcagct cgtcgggcgt gtagcgcgtg ccgtcaggat cctccacctt
     1141 catcgcgtcg atcacgcctt tgaaccgctc gccgaggtcc ttgagcaaga agcgctcgat
     1201 catgacgttg ctcggcacat cgccgcgcag ccgccactcc cgcccatgct tctcgatcag
     1261 ctccgtcttg gccttgagcg cgatctcgtc gaagttgagc ccgctcttca ccccgctgct
     1321 ccctacagca tccgcatccg ccatcgtgtg cgctctctct ccctgctccc tgctcgatca
     1381 gatcccgtgg cctagtacgc ggtgttttgg ttgttcgtca ccgtgatcgt cagcagcttg
     1441 gcctgcgccg gcggccggat ggcggtgaac tgtatctgct ggtccagcgg cttgctgtcc
     1501 agcttcggct ccagcgtcgc cgccgcgaag ttgatcgtcg gcaggctcac cttgaagctg
     1561 ttcacgccgt ccgccgtgat cgtcgtctcc agcgcctctg tccccacgat gaagctgtcc
     1621 gtgtccgccg tctgcgccgc cgctttgccg tagtacgcct ggtcgtactc ctgccacgag
     1681 gagaagtcca ctgtcaggct gccgccggcc gtcagcgcgc ccggcaggaa gtacgccggg
     1741 gtgaggttct cgttgatgag gtcctcggcc gccgtgttgc gcttgagctc caccgagagc
     1801 tgcttgatgg tcgccgcgtt gcccgtcgtc agcgcgttca gcttccacgc ccccgcgccc
     1861 tggtagaaca gcagcggcgg cccgctgtcc tgcaccactg ccgtgaacgc cgcctgcacc
     1921 gacgccatcg cgccgaacca gtcgtgctcg aagatcaccg gcttgccggc atccgcgcgg
     1981 atgcgcagcc cgtagcacac cgcgtccttg acccgcagca cgcggttggg cgtccccccg
     2041 ctcacgccgt acccgaactc gtagctgtac gggtcgtacg tgaacaccgc gctctcgctg
     2101 aacgcatgcg tgctcgggaa cgcgatcggg tccgacgcca gatgcgcctt cttgaaggtc
     2161 ccgctggccg ccagcgtata gacccacgga cccgccccgg tgcgcgtggt gaggtccacc
     2221 gtcgccacct cgtagctcgt gtcgccgtag gccgtcgaga accccaccgc ctgcgacggc
     2281 ccgacgttgc ccaggtcccc cacgctcgtg aacgaggtcg cccccgccgt gatcggcgcg
     2341 gacagcgtgc tgctcttcgc cgccgccgtc agcgtgtccg agttggtccc tagcagcgat
     2401 tgcagcgcgt agcccgcgat caccggccgc gcatactcga tgatcgtgaa ctgccggaac
     2461 tgcgcctgct tgtaggtgag ggtctcggtc tgggtgacgt cgccctccat ctcgttttgg
     2521 agacgcacct gcggcatctc tttggtgccg tcgagccact tgtggaacca cgtcggcgcc
     2581 accgccgtgc cccagttcac ctgcttcgcc ccgcccacgt agaagagatt ccgctggatg
     2641 accgccagcg cattacctgc catctcgccc tatcctcccc ccaccacatc cccgccaatc
     2701 acccgcacca gcgagctcgc catctatcgc actacgccgc gctcagacgg gccctagctc
     2761 gtcacctgct cgacgtagac caccacttcc cacgcgcccc ccagcaccat cagcggcagc
     2821 ttcggatgcg ggtacgtctc gctgcgcccg tagcgcaccg gatcacaccc caccaccgtc
     2881 gccaccgtcg cgtctaagcc gggatgcttc atcaccacct gccacaccgc cgtcaggtag
     2941 cggtccacct gcgcctccag cacgtacagg tcgtcgctct ggcagaccac cgccacctcc
     3001 aggtggtgcg ccagttgccc ccactccgtc gcgtggttca gcgtctgctc tccccccagc
     3061 acgctcacca ccagcgccac cccctccgcc ggcagctgcg cgcgccgcag cgtgtagatg
     3121 ttggcggcgg cgacatgcgg cagcggcacc gtggcggagt ccccccacgc cgctgctatc
     3181 gcgtccacct cggcattgat gtaggtcgcc agcgtgttcg ccagcgccgt cgctgccgcc
     3241 tcgcccccct tcaccgccgt cggcatcgct cgtctcctat ggtgtcgcca ttccgcccga
     3301 cgcctgccgg atgtagtccg ccaccgcccg cacgatcttg ctcttcgtgt accacgagag
     3361 gcccaccagc ttgcgcgccg gcatccgcct ggtgccgaag tgctggaacg tggcatacgg
     3421 gatgctcgtg ccgaacatgg ccgacatcgc cgagacgacg ctgacgttgc ccggcgcgcc
     3481 cagctgcgag agcgagcgcc ccagcatgcc tgtccgccac tccatcggcg cccccggcca
     3541 cctgcgcgcc ttctccttga tggtggaggg tgccagcggc gcccacccgc cgtatctgcc
     3601 gccctccgtt gccatgttca gcccgaagtc cgcccgcagc tcgtccccga tcgccgcgaa
     3661 ggccggcgag aggtccgaga cacgctggtg cagcgcgacg aaacggcggt tgatctcctc
     3721 caccccgtcc acgctcacat gcagctcgaa gaaccccgcc ggtccgctca tgatggtctg
     3781 tgcctccgct cgctacaccc cgctcttggc ctggcccttg tgctgccacg gatcgcgctt
     3841 ggccgccgcc agcagatgcg acgagggccg ccgcggcttg ccgcggtacg tcttgggctt
     3901 cttcggcttc acgctcttgc gatgcgtgcc gtgatagctc atcgtggctc acctgcctcc
     3961 gctgtcgctc gctgtcgctc agaactggtc gtcgcgcttg aacgtcggcc gcgcaatggt
     4021 gaagatcggc gtcgccgcgc tcgtgtccgc gatgctcggg ttggggtaga tcgtcgcccc
     4081 cttcgccaga tcgtagaccg gcagcatcgc ccgcgccgtc gcatcccccc acggatcctc
     4141 gaaccgcagc ttgccgttct cgatgtccgt caccagcagc tcgacgtcct gccgcatcgc
     4201 ctccgccgcc ggcgagaagt ccggctcgga cccgctgaaa taccgcttga tgatgcgcgc
     4261 cgtcgccagc ttggcgttca tctcgcgcag cgtcgtcgtc acgttgaagc tgctgccggt
     4321 gtagggcatc acgtaccagt ggtcgagcat cttctgcagc tccagcgccg cgtacgtgat
     4381 ctcttggccc accacgctgt tggtcagcgt cgagaagctg gccgcgttgg ggaactcgaa
     4441 cttcacgtcg gcgatcgtgg cgtagccgta gtactggccc gccgcgccga acgtcagcgt
     4501 caccgacgcg tccgtgatca ccggcggcga tgcgtccgct gccatcgctg ctcaccacct
     4561 ctctggattg cctactccgg cagcttcttg ccctgcgctt tgttctcgct ctggctgcgc
     4621 accagcgcct ccaactcggc gatgcgattg cgcgccatct ccagctccgc cgcctgctgc
     4681 tgcgccgcgt tgagcgccgc cgccgcctgc tcttccgaga gcgcgatctc gcccggcagc
     4741 ttcaccgcgt tggccgaccg cagcgcgttc cacagcgcct cgtcgtcggt ctcgaaggtc
     4801 tcgccgtggc gatactcgtg caccaccggc gtgccgttgg cgtccacacc ctcgccgtgc
     4861 tcgatgcgcc cctgcgcaat ccctgaaaac ttcgccatac ctctcccctc ttctctgctc
     4921 ttgctactat cgttcgctgg cacgaccccc cgggggcatc acccgcaggg agagctaagg
     4981 cgctcccccg aggagccgcg ctgctactac gcgatggcgg actggatcag gtacgcggcc
     5041 tgcttcgcca ccagcacctc ggtctgcacc aggctggcct ggtagaaggt ggtgtcaatg
     5101 gcctcgtcac gccacgtctt gacctgccag tcacgcgccc ggaagatgct gccagccgtg
     5161 atgctgtcca cacccgggtt ggggttgacg tacgccagga tgacgttctt gccccacacg
     5221 tcggtgtagg tcggcacctc gccctcgacg ctcgtcgagt agatcgccgt cggcatcagc
     5281 accttcatcc cccacagcgt ctgcggcaga tacccgccga cgaggaggtc gggatgcgtg
     5341 tacttgacct gctcgacgat gcccacgtca cgcgccatca cgcgcgccac cgccgccggc
     5401 accacgatgg tgttggcctc caggccgccc gtcgcgctgc ggatgccctc tttgccgtcg
     5461 tcaatgttct tcttgatgac gctctgcgtg gctgtgttct ggctcgcgaa gctcgcgttg
     5521 ttccactggc tcgtgccggc cagcgtcacg aagttggtgc tgtcgtagtt cgccgtggtc
     5581 gtcaggatct tgccgatgcg cagctcctgg ttgagcagga tcttgtcctg cgtgcggcga
     5641 atcttgctgg tctcgagctg cagcacgctg tcggcgttgc cacgctcgcg atccgtcacc
     5701 gtggtgcggc gcgcgaactc atacgcctga tagctcttca gcgtcgcgcc atagctctcg
     5761 gtgcgtgcct cggtgccgtc ggcccgcatg tcgtcgtagc cgtcgccacg ctcgagccgg
     5821 aacgcctgcg cctgatccca gacgtagtac aggtcgttct cgtggttgac ccggatcacc
     5881 gggaagatct gctcggcgat catgcccatc ggatgatagc cgatgctcag ctgtgtcagc
     5941 ggcgcgctga cgtggacgtt gccgaccgtg aagttcggcg atgcctgtgt cgccatctgt
     6001 gtctacctac cccctacccc atcacccatc acccatcacc catcagcagt gcagtcagtc
     6061 agtcaggctc atcgccgatt aggcgttcac ctgcgtcgcc acgcccggca gcaggatcac
     6121 ctcgacgagg tcgcccagcg ccgtcgcgtt gcccttggca atgccgatcg cgtacacctg
     6181 cgagacgccc gccgccggcg ccgtcaccgc acgcccgctg ctgtctacgc tcaccagcgc
     6241 cccataggtg atcgccgcgc cggccaccat cttggtgacg ccatagacca tgatgtcggc
     6301 gctgtcgccc ggcccgatcg ccccggccgt gctgttgccc gccggatacg tgctgttctg
     6361 cagcacgccc aggatcggcg ccgtgttgac cgtcgcgccc ttgatgtcgc cgggattgcc
     6421 ggtcgccgtg tcgaagacca ccgccgtgaa ctgcaccagc ccaccgctgc tatacgccgg
     6481 cggattgccc gtcagtgtca ccgcggtcac gttgctgctc gcctggaacg gcagcgtgaa
     6541 caaacgattc gatgtcgcca tgctctctta cctactcccc cactacccca tcatccacac
     6601 gtgtggttcg tgtctcgccg gctacgagtc tacttgtagc cgacctcttt cgccgcgaga
     6661 tccatgtagc gctcgcggtc ggagagcgcg agcgccgtca gcgtctgccc gcgctcaatc
     6721 ttcttgtcgc tgagcgccag ttgctccgcc gtctcgacga tcctcagctc gtcctcgacc
     6781 ctggcgacgc cgcgcgcatc cgcgccgccg ccctggcgga tcgtgcgccg cgcctcctgg
     6841 tcgaagctcg cgccgaacgt
//
LOCUS       DASUBI010000482         4488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_373446, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000482 DASUBI010000000
VERSION     DASUBI010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373446"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFU72_09280"
     CDS             complement(<1..397)
                     /locus_tag="VFU72_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5423717.1"
                     /translation="MAEARARETATRMEHDLVGERAVPAAAYYGVHTLRALENFPITG
                     TPISIYPELVKALAAVKEAAAQANRELGLLSPEKADAIVRASEEIRQGALLDQFAVDV
                     IQGGAGTSTNMNANEVIANRALELLGHQRG"
     gene            558..1652
                     /locus_tag="VFU72_09285"
     CDS             558..1652
                     /locus_tag="VFU72_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5423718.1"
                     /translation="MSEKRGGAWEVDAAIERASTLPAEVYGDPRYFDLARERIFARSW
                     QFVTDSDRVRHPGQVLPWTMLEGCVDEPLVLTRDADDCLHCLSNVCTHRGNIVVEGEG
                     HAQVLRCRYHGRRFALDGRFLSMPEFEGVAGFPSDADNLPQVPLESWSKFLFTGLDPA
                     FSFDELIAPLRERLGWLSLDAYRYDPISSRDYLVRANWALYCDNYLEGFHIPYIHGGL
                     AGALDYGAYTTEVYEYSNLQLGIAAKGEERFDLPPESPDYGQAVAAYYYWLFPNMMFN
                     FYPGKLSINLVQPLAPDRTRVSFLTYSDGSSPDAGAGAGLDRVEREDEDIVERVQQGI
                     RSRLYHRGRYSPTRETGVHHFHRLLARFLG"
     gene            1723..2613
                     /locus_tag="VFU72_09290"
     CDS             1723..2613
                     /locus_tag="VFU72_09290"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5423719.1"
                     /translation="MILPLLHANAEVNGPFLAAWSFDPLVTLLILLAGFAYGLGLRRV
                     RRGARRAWPTYQAVAFYAGLAVLALALLGPPDTYNDELFSMHMLQHLALLQIAAPLLL
                     LGRPVQLALRAIAPRRSGPVVKAVVGRSWVRWVLGALTAPLIATLLFNLNLVLWHVPD
                     FYTAALNNEHIHELEHALFFGLALLFWWPIIDPVPRHHKAQPAWALASIFASMLFGSA
                     LGAMLTLAHGEIYPTYLSTPKPWGLSPLDDQQIGGLVMWVGGGLLYMSILIAYLVHTF
                     GTGEDSDAAKPATPSTHEIA"
     gene            complement(2755..3372)
                     /locus_tag="VFU72_09295"
     CDS             complement(2755..3372)
                     /locus_tag="VFU72_09295"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU5423720.1"
                     /translation="MPYELPNLPYPYNALEPYIDEQTMNIHHTKHHQAYVNNLNAALQ
                     NNPELQNQPIEQLMRNINQVPESIRQAVINNGGGHANHSLFWTIMGPNKGGEPTGALA
                     DEIKKDFGSFDSFKDEFTKAASGRFGSGWAWLVETPQGKLQITSTPNQNSPLMEGNTP
                     LLGLDVWEHAYYLKYQNRRPEYINAWWNLVDWNAVGQRYQQAKKG"
     gene            complement(3442..4263)
                     /locus_tag="VFU72_09300"
     CDS             complement(3442..4263)
                     /locus_tag="VFU72_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5423721.1"
                     /translation="MVRLAAIGDIHARAGAEETLRGVFQRAQDEADLLLIAGDLTDAG
                     LEEDARRLGRVIDEFDIPIFAVLGNHDFHQGQQNAIRRIVEKHGVHILDGEGVVFEQD
                     GVRLGIAGAVGFGGGFRPYNLTPFGEGEWKQLHGKIVEETRKLDRAFAAVSGADYLIG
                     LTHYAPTAETMGEEPEQLFPYLGSSELGDALERYNAFLGVHGHAHRGRLEGCTANGVP
                     VFNVAMPIVDCPVVWEFKPAAEYQQPPSATPRLLRGRGVEAESQPHPVSAGSQRA"
BASE COUNT          626 a         1604 c         1477 g          781 t
ORIGIN      
        1 cgccgcgctg gtggcccagt agctccagcg cgcggttggc gatgacctcg ttggcgttca
       61 tgttggtcga ggtgccggcg ccgccctgga tcacgtcgac cgcgaactgg tcgagcagcg
      121 ccccctggcg aatctcctcg gaggcgcgca cgatggcgtc cgccttctcc ggtgacagga
      181 gtcccagctc ccggttggcc tgcgcggcgg cctccttgac cgcggccagc gccttgacca
      241 gctcggggta gatcgagatc ggcgtgccgg tgatcgggaa gttctccaac gcgcgcaggg
      301 tgtgcacccc atagtaggcc gcggccggca ccgcgcgctc gcccaccaga tcgtgctcca
      361 tgcgcgtggc cgtctcccgc gccctcgcct cagccatcgg ttgttcctct cccttacgct
      421 caccacgcca gcacggtggc gctatccgcc acaaagctaa ccacatgcta cgaagcgccg
      481 gcctccccat ggtcgcgcgg gagcgccgga cgctttagca gcgtatactg ccccaacatg
      541 gttgaaaggg gaagtctatg agcgagaagc ggggtggcgc ctgggaggtc gacgcggcca
      601 tcgagcgcgc gtcgacgctg ccggccgagg tctacggcga tccgcgctac tttgacctgg
      661 cgcgcgagcg catcttcgcg cgcagttggc agttcgtgac cgatagcgac cgcgtgcgcc
      721 accccggcca ggtgctcccc tggaccatgc tggagggctg cgtcgacgag ccgctggtgc
      781 tgacgcgcga cgccgacgac tgcctgcact gtctctccaa cgtctgcacc catcgcggca
      841 acatcgtggt cgagggcgag gggcatgccc aggtgctgcg ctgccgctac catgggcgcc
      901 gcttcgcgct cgacggccgc ttcctctcga tgcccgagtt cgagggcgtc gcgggcttcc
      961 ccagtgacgc ggataacttg ccgcaggtgc cgctggagtc gtggagcaag ttcctcttca
     1021 ccggactcga cccggccttc agcttcgacg agctgatcgc gccgctgcgc gagcggctgg
     1081 gctggctctc gctcgacgcc tatcgctacg accccattag ctcgcgcgac tacctggtgc
     1141 gcgccaactg ggcgctctac tgcgacaact acctggaggg cttccacatc ccctacatcc
     1201 acggcggcct ggccggcgcg ctcgactacg gcgcctatac caccgaggtc tacgagtaca
     1261 gcaacctgca gctggggatc gccgccaagg gcgaggagcg cttcgacctg ccgcccgagt
     1321 cgcccgacta cggccaggcc gtcgccgcct actactactg gctcttcccg aacatgatgt
     1381 tcaacttcta ccccggcaag ctctcgatca acctggtgca gccgctggcg cccgaccgca
     1441 cgcgcgtctc cttcctcacc tacagcgacg gcagcagccc ggatgccggc gccggagctg
     1501 gactcgaccg ggtcgagcgc gaagacgagg acatcgtcga gcgggtgcag caggggattc
     1561 gctcgcgcct ctaccaccgc gggcgctact cccccacgcg cgagaccggc gtgcaccact
     1621 tccaccgcct gctggcgcgc tttctggggt gagggcctat ggtatccttc ccccggcgaa
     1681 accgcggctg gccgcacgac tggaacagag acgcgccacc cgatgatact ccccctcctg
     1741 cacgccaacg ccgaggtcaa cggacccttc ctggccgcct ggagcttcga tcccctggtc
     1801 accctcctca tcctgctggc cggcttcgcc tatggtctcg gcttgcggcg cgtgcgccgg
     1861 ggagcgcggc gggcgtggcc gacctatcag gcggtcgcct tctacgccgg gctggccgtg
     1921 ctggcgctgg cgctgctcgg gccgcccgac acctacaacg acgagctgtt ctcgatgcac
     1981 atgctgcagc acctggcgct gctgcagatc gccgcgccgc tcttgctgct cggccgcccg
     2041 gtccagctcg ccttgcgcgc catcgcgccc cggcgcagcg gcccggtggt caaggcggtg
     2101 gtgggccgga gctgggtgcg ctgggtgctg ggcgcgctga ccgctccgtt gatcgccacg
     2161 ctgctcttca acctgaacct ggtcctctgg cacgtgccgg acttctacac cgccgcgctg
     2221 aacaacgagc acatccacga actggagcat gccctcttct tcggcctggc tctcctcttc
     2281 tggtggccga tcatcgatcc ggtgccgcgc catcacaagg cacagccggc ctgggcgctg
     2341 gcctcgatct tcgcctcgat gctcttcggc agcgcactgg gggcgatgct gacgctcgcc
     2401 cacggcgaga tctaccccac ctacctcagc accccgaaac cctggggcct gagcccgctg
     2461 gacgaccagc agatcggcgg gctggtgatg tgggttggcg gcggcctgct ctacatgagc
     2521 atcctgatcg cgtacctggt ccacaccttc ggcaccggcg aggacagcga cgcagccaag
     2581 cccgccaccc caagcaccca cgagatcgcg tagtcaacgg gcggtcagcg gcggcgtgcc
     2641 gcgtcccgcg aggctgcgcc gcagggggcg cccgggtgca tacagcgagc gtcatacgac
     2701 agagccgggg cgacgtcgcc ccggctcccg gtgtgcaacg gcgctcgccg ccggctatcc
     2761 cttcttggct tgctggtagc gctgcccgac cgcgttccag tcgaccaggt tccaccaggc
     2821 gttgatgtac tcgggccggc ggttctggta cttgaggtag taggcgtgct cccagacgtc
     2881 gaggccgagg agcggggtgt tgccctccat cagcgggctg ttctggttgg gcgtcgaggt
     2941 gatctgcagc ttgccctgcg gcgtctccac cagccaggcc cagccgctgc cgaagcggcc
     3001 cgaggccgcc ttggtgaact cgtctttgaa gctgtcgaag gagccgaagt ccttcttgat
     3061 ctcgtcggcc agcgcgcccg tgggctcgcc gcccttgttc ggccccatga tggtccagaa
     3121 gagcgagtgg ttggcgtggc cgccgccgtt gttgatgacc gcctggcgga tcgactccgg
     3181 cacctggttg atgttgcgca tcaactgctc gatcggctgg ttctgcagtt cggggttgtt
     3241 ctgcagggcc gcgttgaggt tgttgacgta ggcctgatgg tgcttggtgt ggtggatatt
     3301 catcgtctgc tcgtcgatgt acggctcgag cgcgttgtac gggtacggca gattcggcag
     3361 ctcgtagggc atggcttccc tccctgatcc atctcccgcc gggggcccat tgccacccgg
     3421 ccgatcctac cgatcgctct ctcaggcgcg ctgcgacccg gcgctgacag ggtggggctg
     3481 gctctcggcc tccacccccc gcccacgcag caggcgcggc gtcgcgctcg gcggctgctg
     3541 atactcggcc gccggtttga actcccacac gacggggcaa tccacgatcg gcatcgccac
     3601 gttgaagacg gggacgccgt tggcggtgca accctccagc cggccgcggt gcgcgtggcc
     3661 gtggacgccc aggaaggcgt tatagcgctc cagcgcgtca cccagctcgc tgctgcccag
     3721 gtaggggaag agctgctccg gctcctcgcc catcgtctcg gccgtcggcg cgtagtgcgt
     3781 cagcccgatc agatagtcgg cgccgctgac ggccgcgaag gcgcggtcga gcttcctggt
     3841 ctcttcaaca atcttgccgt ggagttgctt ccactccccc tcgccgaagg gcgtgagatt
     3901 gtaggggcgg aagccgccgc cgaagcccac cgcgccggcg atccccaggc gcacgccgtc
     3961 ctgctcgaag acgacaccct cgccgtcgag gatgtggacc ccgtgcttct cgacgatgcg
     4021 ccggatggcg ttctgctggc cctggtggaa gtcgtggttg ccgaggacgg cgaagatggg
     4081 gatgtcgaac tcgtcgatga cgcggcccaa ccgcctggcg tcctcctcca gccccgcgtc
     4141 ggtcaggtcg cccgcgatca gcagcagatc ggcctcatcc tgcgcccgtt ggaagacccc
     4201 gcgcagcgtc tcctcggcgc cggcccgggc gtggatgtcg ccgatcgcgg caagtcgcac
     4261 catgggctgt cccttccggc tcggtctgcc cgcgacgtag cgcgcgggcg gcacggtctg
     4321 cccggggtat ccagcacgaa ccggaccatg atcgtagcgc ggcggctgcg gcgtgcggta
     4381 ggggaatgcc gaatagtgcg cctgacgcgg cgccgggtgc cagtggtgcc gcggagcctg
     4441 accgccaaac tgcctattcc cgaagtacat cgttcgtcac actccgta
//
LOCUS       DASUBI010000483         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_518219, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000483 DASUBI010000000
VERSION     DASUBI010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518219"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFU72_09305"
     CDS             <1..339
                     /locus_tag="VFU72_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5423722.1"
                     /translation="AKGPRMLEIVARYADTWNSSGTVAELAERNQILDEKCALVGRDP
                     EEITRSLYGWAAIMPDDPWESVDAFQEVIGHYREAGINEFIIDQPPDAKWPVLERVAA
                     EVIPAMRAAG"
     gene            375..1346
                     /gene="corA"
                     /locus_tag="VFU72_09310"
     CDS             375..1346
                     /gene="corA"
                     /locus_tag="VFU72_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU5423723.1"
                     /translation="MIRLELLRHDHSEIENVKAEDLSTVISQPDSLVWIDLVDPTDAD
                     YKLLAQEFDFHPLALEDAQKEGQRPKLDQYPTFIFIIFYAVQVTNDVRAFSTQELALF
                     VGANYLVTVHRQPVPALEEVASRWRQNASNLQGKGVAILLYSVLDTIVDGYFPILDEV
                     AERIDAMEEAIFNRAGKGALQTLFQHRKNLLALRRVIAPERDLTGILVRRDLELLGPA
                     TADYFQEVYDHVLRVTDALDTYRDLLAGALDAYLSVVSNNLNQVMRTLTAWSIILMSV
                     TLIASVYGMNVALWPTNGSILAGIFSLTLMGAIGAALFVLFKGIDWI"
     gene            complement(1356..2123)
                     /locus_tag="VFU72_09315"
     CDS             complement(1356..2123)
                     /locus_tag="VFU72_09315"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5423724.1"
                     /translation="MKIVRFATQQGPRYGVVEGEDVFALNGDIFGQFTAGERVGALGQ
                     LDVLVPVQPSKIVAVGKNYAAHVAEMARGAAGDVPEEPVLFLKPPTALLPDGGVIELM
                     PGYRTDFEAELCMVMAKEAFRVSEDEALGCVLGYTCGNDVSNRQVGGGGQWTRGKGYN
                     TFCPLGPVIETNLDPDHVQVQSRLNGELRQNASTETMIFKPALLVSFISNVMTLLPGD
                     VIMTGTPEGVGSLQPGDVIEITVEGIGTLRNTTRARE"
BASE COUNT          385 a          797 c          725 g          401 t
ORIGIN      
        1 gccaaggggc cgcgcatgct ggagatcgtg gcgcgctacg ccgacacctg gaactcatcg
       61 ggcacggtgg ccgagctggc cgagcgtaac cagatcctgg acgagaagtg cgccctcgtc
      121 ggccgtgacc cggaggagat cacgcgctcg ctctacggct gggccgccat catgccggac
      181 gacccgtggg aatccgtcga cgccttccag gaggtcatcg gccactaccg cgaggccggc
      241 atcaacgagt tcatcatcga ccagccgccc gacgccaagt ggccggtgct ggagcgggtc
      301 gcggccgagg tcatcccggc catgcgcgcg gcgggctgat ccgtgccgat ggcggtgcgg
      361 ttgggatacg cccggtgatc cgactggagc tcctgcggca cgatcactcc gagatcgaga
      421 acgtcaaggc cgaagacctg agcacggtca tcagccagcc cgacagcctg gtctggatcg
      481 acctggtcga tccgaccgac gccgactaca agctgctggc gcaggaattc gacttccacc
      541 cgctggcgct ggaggacgcg cagaaggaag ggcagcgtcc caagctcgat cagtacccga
      601 cgttcatctt catcatcttc tacgcggtcc aggtgaccaa cgacgtgcgc gccttcagca
      661 cccaggagct ggcgctcttc gtcggcgcga actatctcgt gacggtccac cgccagccgg
      721 tgccggcgct ggaggaggtc gcctcacgct ggcgccagaa tgcgtccaac ctacagggca
      781 agggggtggc gatcctgctc tactccgtgc tcgataccat cgtcgacggc tactttccca
      841 tcctcgatga agtcgccgag cgtatcgacg ccatggagga ggcgatcttc aaccgggccg
      901 gcaagggcgc gctacagacg ctctttcagc accgcaagaa cctgctggcg ctgcgccggg
      961 tcatcgcgcc ggaacgcgac ttgaccggga tcctggtgcg ccgcgatctc gaattgctcg
     1021 gcccggctac ggctgactac ttccaggaag tctacgatca cgtgctgcgc gttaccgacg
     1081 cgctcgatac ctaccgcgat ctcctggccg gggcgctgga cgcgtacctt tcggtcgtct
     1141 cgaacaacct gaaccaggtg atgcgcaccc tgaccgcctg gagcatcatc ctgatgtcgg
     1201 tgacgctgat cgcctcggtc tatggcatga acgtggcgct ctggcccacc aacggcagta
     1261 tcctggctgg catcttctcg ctgacgttga tgggcgcgat cggcgccgcg ctcttcgtgc
     1321 tgttcaaggg catcgactgg atttgagcgc ccgccttact cgcgcgctcg cgtggtgttg
     1381 cgcagcgtgc cgatcccttc gaccgtgatc tcgatcacgt cgccgggctg cagcgagccg
     1441 acgccttcgg gcgtgccggt catgatcacg tcgcccggca gcagcgtcat cacgttgctg
     1501 atgaagctga ccaggagggc cggcttgaag atcatcgtct cggtcgaggc gttctgccgc
     1561 aattctccat tgagccgcga ctgcacctgg acgtggtcgg ggtcgaggtt cgtctcgatc
     1621 accgggccca gcgggcagaa ggtgttgtag cccttgccgc gcgtccactg gccgccgccg
     1681 cccacctgcc ggttgctgac gtcgttgccg caggtgtagc ccagcacgca gcccagcgcc
     1741 tcgtcctcgg agacccgaaa agcctccttc gccatgacca tacagagctc ggcttcaaag
     1801 tcggtgcgat agcccggcat cagctcgatc accccgccgt cgggcagcag cgcggtcggt
     1861 ggcttgagga agagcaccgg ctcctccggc acgtcgccag ccgcgccgcg cgccatctcg
     1921 gccacatggg cggcgtaatt cttccccacc gctacgatct tcgacggctg caccggcacc
     1981 agcacgtcga gctggccgag cgcgcccacg cgctccccag ccgtgaactg gccgaagatg
     2041 tcgccgttca aggcaaacac gtcctcgccc tcgaccacgc cgtagcgcgg cccctgctgc
     2101 gtggcaaagc gcacgatctt catcccggca ctcctttcac ccgcatggcc cttctacctg
     2161 gggcacgcat cgctgttcac tcaatcccat agtcaggtgc tcgactccgc tcgacagaca
     2221 atagacgcgg gtgcggaccc ggtcaatctg aggcacggag gtgaagcgcg tgccgcgtga
     2281 ggttgtcacg cggccggcgt gcgatcat
//
LOCUS       DASUBI010000484         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_149178, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000484 DASUBI010000000
VERSION     DASUBI010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149178"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFU72_09320"
     CDS             <1..362
                     /locus_tag="VFU72_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan hydrolase"
                     /protein_id="HEU5423725.1"
                     /translation="EVRRLGLTGSPAFRPMPANAAAAPGCETFPETGHSLCGLFRDYW
                     HGHGLEFGDSGVSYRESLALFGYPISQPFTDPASGLTVQYFERARFEYHPENAAPYDV
                     LLGLLGNDELQAKGWIR"
     gene            359..835
                     /gene="aroQ"
                     /locus_tag="VFU72_09325"
     CDS             359..835
                     /gene="aroQ"
                     /locus_tag="VFU72_09325"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU5423726.1"
                     /translation="MSESAGRTVLVLHGPNLNLLGKREPAIYGTTTLEEINQAIRALA
                     AEHGCDVVITQSNHEGTIVDEIQHYGFSACGIVINPGALTHYSIAVRDALAAVPAPAI
                     EVHLSNIYTREPFRHESVIAPVVTGQIAGLGASGYLLALRYLMETARNAQSADRPA"
     gene            807..1904
                     /locus_tag="VFU72_09330"
     CDS             807..1904
                     /locus_tag="VFU72_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5423727.1"
                     /translation="MHSRLTALREALAQAELDAIVITNPFNRRYLTGFEAEDHPPNES
                     AGHVVVSQERAVLVVSPLEATRAREQAPDFETFDKLRPLAAGDAEILKQIGAKRVGIE
                     SDAILHADFVVLERELGEQAEFHDASDLVPNLRVFKSPDELALIERAIAITDQALEQV
                     AADLRAGETERELAGRLDAAMRAFGATGNAFATIVAAGPNSALPHHTPGERRIQPGEP
                     IVIDMGAEYNGYCADLTRTVWVGEPNELLRQIYPVVMRALDAAEEHLHAGLTGREADA
                     IARQVIEQAGYGDYFPHSLGHGVGVQIHERPGLSARSEQPLEPAQVVTIEPGIYIPGQ
                     GGVRIEDVAIIEANDVRVPTRATKNHIDEEK"
     gene            1914..2474
                     /gene="efp"
                     /locus_tag="VFU72_09335"
     CDS             1914..2474
                     /gene="efp"
                     /locus_tag="VFU72_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5423728.1"
                     /translation="MIDTGGLRKGLTLDMNGELVKVVDYQHVKQGRGSAFVRLTLRNL
                     RTGSTTQQTYQAGSRFQTARMERQRVQYLYNEGDQFHFMDVDTFEQFPLDREALGGAV
                     DYLLENETIDLLTYQGQPVDVEIPITVNLEVVETEPGVRGDTASGGSKPAKLQTGVTV
                     SVPLFVNEGDVVKVDTRSGQYIERVS"
     gene            2477..2950
                     /gene="accB"
                     /locus_tag="VFU72_09340"
     CDS             2477..2950
                     /gene="accB"
                     /locus_tag="VFU72_09340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5423729.1"
                     /translation="MATHAEAGSATDEYRALAQLARELSEIMREGGLSKLELRHGELS
                     IELHSAAGQPPATMPAQATSPASEPTPDGRAEPGGYLISSPMIGTFYAAAAPGDRPFV
                     EVGDRVEPGQTVAIIEAMKIMNEIVSERGGVVTEIFVTNGEAVEYGHPLLRLREE"
     gene            2957..>3468
                     /gene="xseA"
                     /locus_tag="VFU72_09345"
     CDS             2957..>3468
                     /gene="xseA"
                     /locus_tag="VFU72_09345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5423730.1"
                     /translation="MRVLDVAELTAYLKDLLESDPILGDVLVRGEISNFTRSAAGHLY
                     FSLKAEGVQLRCVCFRGDARYLSFQPANGDAVVAHGHISLYEASGQYQLYVNVLVPEG
                     TGLLQVQFEQLRQQLEREGLFEPSRKRPLPAFPRRIGVVTSPTGAVLQDIRNVIGRRW
                     PLAHLVLAPTA"
BASE COUNT          587 a         1234 c         1132 g          515 t
ORIGIN      
        1 cggaggtacg gcggttggga ctcaccggct cgcccgcctt tcgccccatg ccggcgaacg
       61 ccgccgcggc accgggttgt gagaccttcc ccgagaccgg ccactcgctc tgtggcctct
      121 tccgcgacta ctggcacggc cacgggctgg agttcggcga ctccggcgtc tcctatcgtg
      181 agtcgctggc gctcttcggc tacccgatca gccagccttt caccgacccg gccagcggcc
      241 tgacggtgca atacttcgag cgggcgcgct tcgagtacca tccggagaac gccgcgccct
      301 acgacgtgct cctcggcctg ctcggcaacg acgagctgca ggcgaaaggc tggatccgat
      361 gagcgagagc gccgggcgca ccgtcctcgt cctccacggt ccgaacctca atctgctggg
      421 gaagcgcgag cccgccatct acggcaccac cactctggag gagatcaatc aggcgatccg
      481 cgcgctggcg gccgagcacg gctgcgacgt ggtgattacg cagtcgaacc acgagggcac
      541 gatcgtggat gagattcaac actacggctt ctccgcctgt ggtatagtca tcaatccggg
      601 ggcgctgacc cactacagca tcgcggtgcg cgacgcgctg gcagccgtgc cggctccggc
      661 catcgaggtc cacctgagca acatctacac gcgcgagccg ttccgccatg agtcggtgat
      721 cgcccccgtg gtgaccggcc agatcgccgg cctgggagca agcgggtacc tgctcgcctt
      781 gcgctatctc atggagaccg ccaggaatgc acagtcggct gaccgccctg cgtgaggccc
      841 tcgcgcaggc tgagctggat gcgatcgtca tcaccaaccc cttcaaccgc cgctatctca
      901 ccggcttcga agccgaggat cacccgccaa acgagtcagc cggccacgtc gtcgtcagcc
      961 aggagcgcgc cgtgctggtc gtcagcccgc tggaggccac gcgcgcccgc gagcaggcgc
     1021 cggatttcga gaccttcgac aaactgcgcc cgctggcggc cggcgacgcc gagatcctga
     1081 agcagatcgg cgccaaacgc gtcggcatcg agtccgacgc tatcctgcac gccgacttcg
     1141 tcgtgctgga gcgcgagctg ggcgagcagg ccgagttcca cgacgccagt gatctggtgc
     1201 ccaacctgcg cgttttcaag tcacccgacg agctggcgct gatcgagcgc gccatcgcca
     1261 tcaccgacca ggcgctggag caggtcgccg ccgatctgcg cgccggggag accgagcgcg
     1321 agctggcggg gcggctggac gccgccatgc gcgccttcgg cgccaccggc aacgccttcg
     1381 ccaccatcgt cgccgccggc cccaactccg ccctgccgca ccatacgccg ggcgagcgcc
     1441 gcattcagcc cggcgagccg atcgtgatcg acatgggcgc cgaatacaac ggctactgcg
     1501 ccgacctgac ccgcacggtc tgggtgggcg agccgaacga gctgctgcgc cagatctacc
     1561 cggtcgtgat gcgcgcgctc gacgccgcgg aggaacacct gcatgcgggg ctgaccgggc
     1621 gcgaagcgga cgccatcgcg cgccaggtga tcgagcaggc cggctacggc gactacttcc
     1681 cgcactcgct ggggcacggc gtcggcgtgc agatccacga gcgccccggc ctctcggccc
     1741 gctcggagca gccgctggag ccggcgcagg tcgtcaccat cgagccgggc atctacattc
     1801 ccggccaggg tggggtgcgt atcgaggatg tcgctatcat cgaggccaac gacgtgcgcg
     1861 tgcccacccg cgcgacgaag aatcacattg atgaggagaa gtaattcgtg gcaatgatcg
     1921 atactggcgg cctgcgcaag ggcttgacgc tcgacatgaa cggcgagctg gtcaaagtcg
     1981 tcgactacca gcacgtcaag caggggcgcg gcagcgcctt cgtccgactc acgctgcgca
     2041 acctgcgcac tggctcgacc acccagcaga cctaccaggc cggctcgcgc ttccagaccg
     2101 cccgcatgga gcggcagcgc gtccaatacc tctacaacga aggcgatcag ttccacttca
     2161 tggacgtgga taccttcgag caattcccgc tcgaccggga ggcgctgggc ggcgccgtcg
     2221 actacctgct ggagaacgag acaatcgatc tcttgacgta ccaggggcag ccggtcgacg
     2281 tcgagatccc gatcacggtc aacctggagg tcgtggagac cgagcccggc gtgcgcggcg
     2341 acactgcctc gggcggcagc aagcccgcta agctgcagac cggcgtcacc gtaagtgtgc
     2401 cgctcttcgt caacgagggc gacgtggtaa aggtcgacac ccgcagcggg cagtatatcg
     2461 agcgcgtgag ctagcggtgg cgacgcacgc ggaggccggc tccgccaccg acgagtaccg
     2521 cgcgctggcc caactggcgc gcgagctgtc cgagatcatg cgcgagggcg gcctcagtaa
     2581 gctggagctg cgccacggcg agctcagcat cgagctgcac agcgccgccg gccagccgcc
     2641 agcgacgatg ccggcgcagg cgacctcccc cgccagcgag ccaacccccg acgggcgtgc
     2701 ggagccgggc ggctacctga tcagctcccc gatgatcggc accttctacg ccgcggccgc
     2761 acccggcgat cgtcccttcg tggaagtcgg cgaccgggtc gagccggggc aaaccgtcgc
     2821 gatcatcgaa gcgatgaaga tcatgaacga gatcgtgtcc gagcgcggcg gcgtggtgac
     2881 cgagatcttc gtcaccaacg gcgaggcggt cgagtacggt catccactgc tacgcctgcg
     2941 cgaggagtag ccggccatgc gcgtgctcga cgtcgccgag ctcacggctt acctcaagga
     3001 tctgctcgag tccgacccga tcctgggcga cgtcctggtg cgcggcgaga tctccaattt
     3061 cacccgctcg gccgccggtc acctctactt cagcctgaag gcagagggcg tgcaactgcg
     3121 ctgcgtctgc tttcgcggtg acgcgcgcta cctttccttc caaccagcca acggcgatgc
     3181 cgtcgtcgcg cacggccaca tttcgctcta cgaggccagc ggccagtacc agctttatgt
     3241 caatgtgttg gtgcccgagg gcaccggcct gctgcaggtg cagttcgagc agttgcgcca
     3301 gcagctagag cgcgaggggc tcttcgaacc gagccgcaaa cggccgctgc cggccttccc
     3361 gcgccgcatc ggcgtggtca cctcgcccac cggcgccgtg ctgcaggaca tccgcaacgt
     3421 gatcggccgc cgctggccgc tggcgcacct ggtgctggcg cccaccgc
//
LOCUS       DASUBI010000485         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1226807, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000485 DASUBI010000000
VERSION     DASUBI010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226807"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="mutS"
                     /locus_tag="VFU72_09350"
     CDS             <1..1285
                     /gene="mutS"
                     /locus_tag="VFU72_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5423731.1"
                     /translation="KVGYNKVFGYYLEVTKPNLAHVPADYLRKQTLSHCERFITPELK
                     ECEARISDAESRITAVEDAIYARLLDDLALSAPRLLAVAAASAQLDVLAAFAEVADQR
                     GYTRPALDTSTALHIRAGRHPTVEAALEQHAFIPNDLDLDAGETGDGCIMLLTGPNMA
                     GKSTYLRQTALIVLLAQIGAFVPAAEARIGIVDRIFTRVGAQDDLAAGQSTFMVEMLE
                     TAAILHHATPRSLIVLDEIGRGTGTLDGLAIARAVIEDIHDRIGARCLFATHYHELLA
                     LLDALPRLRACNVAVREEADGIVFLHRIAPGGAARSYGIHVARLAGLPATVTSRADAL
                     LRDLEARAAVAAELPAGDTVTTEPPPALLPVRQPAHEPAWEGDPGEILDEIAALDLIN
                     TTPLDALNRLFAMQQRLRALDDLPVRTPRHQRRRG"
     gene            1590..>2285
                     /locus_tag="VFU72_09355"
     CDS             1590..>2285
                     /locus_tag="VFU72_09355"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5423732.1"
                     /translation="MLFTPLCARLGIDHPVLNAPMSGTATADLAAAVSEAGGFGMIGG
                     TSRGGPDWLRAQIHAVREKTDHPFGVGFISSFPGLDDLVEVALEEGVAAINHSFADPT
                     PYVAPAHARGVRVFVQVQTVAQATQAARAGVDVIVAQGTEAGGHTGQAGTMALVPAVV
                     DAAGDIPVVAAGGIADGRGLAAALLLGATGGWLGTRFVASAEWGGSAWEQQAVLAATA
                     DDTVRTSVFDQLRG"
BASE COUNT          353 a          804 c          788 g          340 t
ORIGIN      
        1 gaaggttggc tacaataagg tcttcggcta ctacctggaa gtgacgaagc cgaacctggc
       61 ccatgtgccc gccgactacc tccgcaaaca gaccctcagt cattgcgagc gcttcattac
      121 gcccgaactg aaagagtgcg aggcgcgcat cagcgacgcg gagagccgga tcaccgctgt
      181 ggaagacgcg atctacgccc ggttgctgga cgacctggcg ctatcagcgc cgcgcctgct
      241 cgccgtcgcg gcggccagcg cgcagctcga cgtgctcgcc gccttcgccg aggtcgcgga
      301 ccagcgcggc tacacccggc ccgcgctcga taccagcacg gcgctgcaca tccgcgcggg
      361 gcgtcacccg acggtggaag cggcgctgga gcaacacgcc ttcattccca acgatttgga
      421 tcttgacgcc ggtgaaaccg gcgacggctg catcatgctc ctgaccgggc cgaacatggc
      481 cggcaagagc acctatttgc ggcagacggc gctgatcgtc ctgctggcgc agattggcgc
      541 gttcgtcccg gcggccgaag cgcgcatcgg catcgtggac cgtatcttca cgcgcgtcgg
      601 cgcgcaggac gacctggccg ccggccagag caccttcatg gttgagatgc tggagacggc
      661 ggccattctc caccacgcga cgccgcgcag cctgatcgtg ctggacgaga tcgggcgcgg
      721 caccggcacg ctcgacggcc tggcgattgc ccgtgcggtg atcgaggaca tccacgaccg
      781 gatcggcgcc cgctgcctct tcgccacaca ttatcacgaa ctgctggcat tacttgacgc
      841 gctgccccgc ctgcgcgcgt gcaacgtcgc ggtgcgcgag gaagcggacg gtatcgtctt
      901 cctgcaccgt atcgcgcctg ggggtgcggc gcgctcctac ggcatccacg tcgctcgcct
      961 ggccggccta ccggccaccg tcaccagccg cgccgacgcc ctgctgcgtg acctggaagc
     1021 ccgcgccgcc gtcgcggccg agctgccagc tggcgacacc gtcaccaccg aaccaccgcc
     1081 cgccctgctg ccagtgcgcc agccagcgca cgagccggcg tgggaaggcg accccggcga
     1141 aattctcgac gagatcgccg ccctcgacct gatcaacacc acgccgctcg acgcgctgaa
     1201 ccgcctcttc gcaatgcagc agcgcctccg cgcgcttgac gacctgccgg tgcgaacgcc
     1261 ccgccaccag cgccggcgcg gctgaacagc ctacctgatt gctcggcatc gcgggcacag
     1321 gtgcggacga ggagggagac gtgaaccttg agttccatgg ggtctccgcc gtgcaagagg
     1381 gtccgaagca ctcctggtgt ccatagagcg ccccagggcg gccacaagcc agagtatgct
     1441 tgggtgtaaa ttgcgcgtgc tggcgggtgt cgcggatcga gcgggtggga gcatgccaca
     1501 caacctgcta gactcgcgac gagattagga cgtgttcgcg cgccgcaacc catgtgcgcc
     1561 tgggtgtgtg gccggcgaag gaggtgatca tgctgttcac cccgctgtgc gcccgcctgg
     1621 gcattgacca cccggtgctg aatgccccga tgagtggcac ggccactgcc gatctggccg
     1681 ccgctgtctc ggaggcgggc ggcttcggca tgatcggcgg caccagtcgc ggcgggccgg
     1741 actggctccg cgcccagatc cacgccgtgc gcgagaagac cgaccacccc ttcggggtgg
     1801 gcttcatctc ctcgttcccc gggctcgacg acctggtgga ggtcgccctg gaggagggcg
     1861 tggcggctat caaccactcc ttcgccgatc cgacgcccta cgtcgcaccg gcccatgcgc
     1921 gcggcgtgcg tgtcttcgtc caggtgcaga ccgtggcgca ggcgacccag gcagcgcggg
     1981 ccggcgtgga tgttatcgtc gcacagggga ccgaggcggg cgggcacacc ggccaggcgg
     2041 gcaccatggc gctggtgccg gcggtggtgg acgcagccgg agacatcccc gtggtggctg
     2101 cgggcggcat cgccgatggc cggggcctcg ccgccgcgct gctcctgggc gcgacgggcg
     2161 gctggctggg gacgcggttt gtcgccagtg cggaatgggg cggcagtgcc tgggaacagc
     2221 aggcggtcct ggccgcgacg gccgacgaca cggtgcggac ctcggtcttc gaccaactgc
     2281 gcggc
//
LOCUS       DASUBI010000486         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1203767, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000486 DASUBI010000000
VERSION     DASUBI010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203767"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFU72_09360"
     CDS             complement(<1..193)
                     /locus_tag="VFU72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5423733.1"
                     /translation="MSSSANTTEMLEEMRTTRQIRLYHPKPVPQDVLQQLLEVARWSG
                     SSRNSQPWHFIVVTDKDELR"
     gene            423..1007
                     /locus_tag="VFU72_09365"
     CDS             423..1007
                     /locus_tag="VFU72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5423734.1"
                     /translation="MASFSFLSLWELTAPIEHVWAEVSRPEEYPRWLPYVVSVEQVSP
                     GDAAGIGATHVSRWKTALPYGFAFQTRVIRSEPPHLHVLESSGDLEGTGRWELQANVT
                     GTTVRYYWQVRPTQSWFNLIAPLARPAFEWNHDVIMHAGGEGLARLLGARLTRNEAFS
                     ASSTSPLRPIVSILGPIALAIMLVRQLRRLRPGG"
     gene            1059..1598
                     /locus_tag="VFU72_09370"
     CDS             1059..1598
                     /locus_tag="VFU72_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423735.1"
                     /translation="MQDASRSSGSAGSVETGGEARSGWLSAVVHNLYGHPEQMAPPSS
                     LVERGGYRRARDVIVARRLQQPVVTTIKLAHTAIFALLMSLVVSVALAGIRDRFTRRT
                     LAALLFVVAEAAVVTLNGGGCPLTSVVEDLGAEHGSVSDIFLPDWFARRIPHISTALL
                     LLGMTALAARRLARLARPA"
     gene            1805..>2157
                     /locus_tag="VFU72_09375"
     CDS             1805..>2157
                     /locus_tag="VFU72_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423736.1"
                     /translation="MVLALLFTWSISIPLALSDRGVLPLDLPLALHYLTPFGPLAASL
                     LTLALTSGRAGLRDLGRRVVRWRVGVAWWLAALSPVLLFTGAALMDRLLNGTSPTLSA
                     IGRVNFLPALGLAAIP"
BASE COUNT          307 a          772 c          712 g          366 t
ORIGIN      
        1 tgcgcagctc gtctttatcg gtgacgacga tgaagtgcca gggctggctg ttgcgcgagc
       61 tgccggacca gcgcgccacc tccagcagtt gctggagcac atcctggggc accggcttcg
      121 gatggtagag ccggatctgc cgcgtggtgc gcatctcctc caacatctcg gtggtgtttg
      181 ccgaactgct catcgagcac cctccctcat ggccgcgccg ctccacgcgg cgccgggcgg
      241 acccaacaga acccatcata cggcagcctc cggcgattct cgcgattcga tatctcttac
      301 gcccacgttg agttatcatg ccgcccgagt acgccacatc gacgacccgg gggatctcgg
      361 gcgtgcgggt gcagtgaacg ttcatcggcc gcggcgcgaa gcagcgcaag ggaggcgcga
      421 ccatggcctc tttctcgttc ctctcgctct gggaattgac cgctccgatc gagcacgtat
      481 gggccgaggt gagccggccg gaggaatacc cgcgctggtt gccctatgtc gtctccgtgg
      541 aacaggtcag ccccggcgac gcggccggga tcggcgccac gcacgtcagc cgctggaaga
      601 cggccctgcc ctatggcttc gcgttccaga cacgcgtgat ccgctcggag ccaccgcatc
      661 tgcatgtgct ggaatcgagc ggcgacctgg aaggaaccgg tcgctgggag ctgcaagcga
      721 acgtgaccgg caccacggtg cgctactact ggcaggtgcg cccgacccag tcgtggttca
      781 acctgatcgc gccgctggca cggccggcgt tcgagtggaa tcacgacgtc atcatgcacg
      841 cgggcggcga ggggttggca cggctgctcg gcgcgcggct tacccgcaac gaggcgttct
      901 ccgcgagctc aaccagcccg ctccgcccga tcgtcagcat cctggggccg atagctctgg
      961 ccattatgct ggtccgccag ctgcggcgcc tgaggcccgg cggctgagtc cgaccaagct
     1021 cggggaaccg ccgaacgccg tcgcgatagg agcaattgat gcaggacgcg tcgcgctcgt
     1081 ccgggtctgc cggctcggtc gaaaccggcg gcgaagcccg ctcgggctgg ctatccgcgg
     1141 tcgtccacaa cctctatgga caccccgagc agatggcgcc gccaagtagc ctcgtcgagc
     1201 gcggcggcta tcgccgggcg cgggacgtga tcgtcgcgcg ccggctgcaa cagccggtcg
     1261 tgacgactat caagctggcg cacaccgcga tcttcgcact cctcatgagt ctggtcgtca
     1321 gcgtcgccct ggcgggcatc cgcgaccgct tcacccggag aacgctggcc gcgctgctct
     1381 tcgtcgtggc ggaagcggcc gtggtgacgc tgaatggggg cggctgcccg ctgacttcgg
     1441 tggtcgaaga cctgggcgcg gagcacggct cggtgtcgga tatcttcctg cccgactggt
     1501 tcgcccggcg catccctcat atctcgaccg cgctcctgct gctgggcatg accgcgctcg
     1561 ccgcgcggcg cctcgctcgc ctggcgagac cggcgtagcg cgcggccgct cgacctccca
     1621 catccccacg ccaattgttg ccgagttgca acatctcatg cccccccgcc acgtgcgtga
     1681 cacagctgga ggcgagactt gagccggaag ggctggccga tcgagccggc cagcccaatc
     1741 ggagggagcc aggcgccgac catccggcgt accgatcgcc gtccgccatc ccacccagac
     1801 cttcgtggtg ctggcgctcc tcttcacctg gagtatcagc atcccgctcg ccctctcgga
     1861 tcgtggcgtc ctgccgctcg atctgccgct cgcgctccac tacctcaccc cgttcgggcc
     1921 gctggccgca tcgctgctga cgctcgccct gaccagcggg cgcgcgggcc tgcgtgacct
     1981 cggccggcgc gtggtgaggt ggcgcgtggg ggtggcctgg tggctggcgg cgctctcgcc
     2041 ggtgctcctc ttcaccggtg ccgcgctgat ggatcggctg ctgaacggca cctcgcccac
     2101 actaagcgct atcggccggg tcaacttcct gcccgccctg gggctggcgg ccatccc
//
LOCUS       DASUBI010000487         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_755940, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000487 DASUBI010000000
VERSION     DASUBI010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755940"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2417)
                     /locus_tag="VFU72_09380"
     CDS             complement(<1..2417)
                     /locus_tag="VFU72_09380"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5423737.1"
                     /translation="MKRLRLAAALTQEALAERAGISAKAVSDLERQPTRLPRLESVSL
                     LADALHLDAEQRAGLVAAARPPRSSAVAPAEHGQRRHSLPRPLTPLVGRAFDVATIEA
                     LVRGGEARLLTLIGPGGVGKTRLAIAVAGRVADGLADGAVLIDLTPLNDPALVIPAIA
                     RQLGLREDDPTPLARRLAEYLEPRRLLLVLDNFEHVIAARTAILDLLTACPRLVALVT
                     SREALRLSGEHVFAVAPLALPEPATSPASLIFAPAVELFVQRARAAGAPLASDDPNLP
                     SVAEICRRLDGLPLAVELAAAWTPLLAPATLLRRLETRLPLLVGGPYDSPQRQRTMRE
                     AIAWSYDLLDARQQRLFRALCVFEGGCTVEAAAAVAEEDATAMLRGLAELAAKSLVRL
                     TTPPARQPAGEEPRVAILATIREYGLEQLAAAREADLLQRRHLAYFLALAGRAEGELA
                     GPEQASWLRELEDDHDNLRAALRWALETGETAAARRLTAAMWPFWSLRGYLSEGRRWL
                     GAALTAEPPAPDDAAADALRATLLLGATTLAIDQGDLAEAAELSDRGLTHARARGAAG
                     ELIAALDARGRLERERGHYREAEQAHQEALSLANAAGDQAGAALALAGLGTVASLSGD
                     LPRATAFFDQSLTRFRELGNVRRIAEIQGDLARQLHLLGDLERAIALMEEALPVFQAL
                     GDTSGVANVLFAVGVVAASRGQYDQGEDLLRQSVVIWRERGDERHVAQALAGLGSVAI
                     HREDHAAAGALLEKALAIARRFDDAWALGIHLTLLAIAELGDGRPRRARERLAEAIHL
                     FRAIGNP"
BASE COUNT          373 a         1018 c          978 g          345 t
ORIGIN      
        1 tgaggattgc cgatggcgcg gaagaggtgg atagcctccg cgagccgctc acgcgcgcgc
       61 cgcggtctac cgtcgccaag ctcggcgatg gccagcaacg tcaggtggat tcccaacgcc
      121 caggcatcgt cgaagcggcg cgcgatggcc agcgccttct ccagcagcgc gcccgccgcc
      181 gcgtggtcct cacgatggat cgcgaccgac cccaacccgg ccagggcctg ggccacgtgt
      241 cgctcatcgc ctcgctcacg ccagataacc acgctctgcc gcaggagatc ctccccttga
      301 tcgtactgac cacggctggc cgcgaccacg ccaacggcga acagcacgtt ggccacgccg
      361 ctcgtgtcgc cgagcgcctg gaagaccggc agcgcttctt ccatgagtgc gatggcccgc
      421 tccaggtccc cgagtaggtg cagttgccgc gccaggtcgc cctggatctc ggcgatgcgc
      481 cggacgttcc ccagctcgcg aaagcgggtc aggctctgat cgaagaacgc ggtcgcccgc
      541 ggcagatcgc ccgagaggct ggccaccgtg ccaagcccgg ccagcgccag ggcggcgccg
      601 gcctgatccc cggccgcgtt cgccagcgag agcgcctcct gatgggcctg ctcggcctcg
      661 cggtagtggc cccgctcacg ctcgagtcgc ccgcgggcgt cgagggcggc gatcagctcc
      721 ccggccgcgc cgcgcgcccg ggcatgcgtc agaccgcgat ccgagagctc ggccgcttcc
      781 gcgaggtccc cttgatcgat ggccagcgtc gtcgcgccga gcaagagtgt cgcccggagc
      841 gcgtcagccg ccgcgtcatc gggcgccggg ggctccgcgg tcagcgcggc ccccagccag
      901 cgccgcccct cgctgaggta gccgcgcaag gaccagaacg gccacatggc cgccgtcagt
      961 cgccgtgccg cggccgtctc cccggtctcg agcgcccagc gcaacgccgc gcgcaggttg
     1021 tcgtggtcgt cctcgagctc ccgcaaccag gacgcctgct cggggccggc caactccccc
     1081 tcggcacgcc cggccagcgc caggaaatag gccaggtgcc ggcgctggag cagatccgct
     1141 tcccgcgcgg cggccagttg ctcgagcccg tactcgcgga tcgtcgccag gatggccacg
     1201 cgcggttcct cgcccgctgg ctgccgcgcg gggggtgtcg tcagccgcac cagactcttg
     1261 gcggccagct cggccagccc acgcagcatg gcggtggcgt cctcctccgc gacggccgcg
     1321 gctgcctcta ctgtgcagcc gccctcgaag acgcacagcg cgcgaaagag gcgttgctgc
     1381 cgcgcatcca gcaggtcgta gctccaggcg atcgcctcgc gcatcgtccg ctggcgctgc
     1441 ggcgagtcgt agggaccgcc gaccagcagc ggcaagcgag tctccaaccg ccgcaggagc
     1501 gttgcgggcg ccagcagcgg cgtccaggcg gcggccagct cgaccgccag cggcagcccg
     1561 tcgagccgcc ggcagatctc ggccacgctc ggcaggttcg ggtcgtcgct ggcgagcggc
     1621 gccccggccg cgcgtgcccg ctggacgaag agttcgaccg ccggggcgaa gatgaggctc
     1681 gccggcgagg tcgcgggctc gggcagtgcc aggggcgcca ccgcgaagac gtgctcgccg
     1741 ctgaggcgca gcgcctcgcg gctggtcacc agcgcgacga gccgcgggca cgccgtgagc
     1801 aggtcgagga tcgcggtgcg ggccgcgatc acgtgctcga agttgtccag caccagcagg
     1861 agccggcgcg gctcgaggta ctcagccagg cggcgggcca gcggcgtggg atcatcctcg
     1921 cgcaggccga gctggcgggc gatggcgggg atgaccagcg ccgggtcgtt gaggggcgtc
     1981 aggtcgatca gcacggcccc gtcggcgagg ccgtccgcga cgcgcccggc cacggcgatg
     2041 gccaggcggg tcttgccgac gccgccgggt ccgattagcg tcagcagccg cgcctcgccg
     2101 ccgcgcacca gtgcctcgat ggtcgccacg tcgaaggcgc ggccgacgag cggcgtgagc
     2161 ggtcgtggca ggctgtgccg ccgctgaccg tgctctgccg gagccaccgc gctgctccgc
     2221 ggcgggcgcg cggccgctac cagcccggcc cgctgttcag cgtcgaggtg gagcgcgtcg
     2281 gccagcagcg agacgctctc cagccgtggc agccgggtcg gctggcgctc cagatcgctg
     2341 accgccttgg cgctgatccc ggcgcgctcg gcgagagctt cctgcgtcag cgcggccgcg
     2401 aggcgcagcc gcttcaacac ggctccgaag ggctcggaag ccgggtgcgc cgcatcgtct
     2461 ccagctgctc gtggagccat ggacacctcg cgctgctcgg ctcggcacgc tgctcgcggg
     2521 cgtggcacct ctgagagaac gtagccaatt gtaacaagcg cgtgccatcc tcacgtccgg
     2581 cgccggccgc gcgatgtctc cccaacatcc gatgaacggg gagcgcgcgt cctgttgtgc
     2641 cccctggcgc gagcggagaa tcagatacga gcgcgacggt gaagcacgcc ggaccggcaa
     2701 ccggcaggca actg
//
LOCUS       DASUBI010000488         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_659218, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000488 DASUBI010000000
VERSION     DASUBI010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659218"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1662)
                     /gene="gltX"
                     /locus_tag="VFU72_09385"
     CDS             complement(145..1662)
                     /gene="gltX"
                     /locus_tag="VFU72_09385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5423738.1"
                     /translation="MNTTERRVRVRMAPSPTGDLHVGGARTALFTWLYARHHDGDFIL
                     RIEDTDRKRFVEQSLSGILEAFRWLGLDWDEGPEVGGPKGPYIQSERLELYQHYAHEL
                     VERGHAYYCFCTPERLSALRDEQRARHEPPGYDRHCRNLSAQEIAELRARNVPTAIRF
                     KMPDSGTTAVDDLLRGQIAFENRLLEDPIILKSDGYPTYHLAVVVDDHLMEITTVTRG
                     EEWIPSAPIHVQLYQAFGWEPPAFAHLPVILRPDGKGKLSKRDGDVGVLQYKHAGYLP
                     EALVNYLALLGWSSGDQEIFTRQELIEKFALRDVSPSPARFNFEKLAWMNQYYINHVL
                     TLDDLTARCVPYLQQAGLAGPAASEPGSPEHAYVRSVVALVKDRLKLLPEVVELTDFF
                     FTAEPEPYDPDLLIPRKTDPELVVRALEAALGLLEQADFDDETALDVRLRALAGELDL
                     KAGQLFMPIRVAVTGRTVSPGLFETLRVLGKERSLARLRQALIKLQRRLKDNAAD"
     gene            1855..2625
                     /locus_tag="VFU72_09390"
     CDS             1855..2625
                     /locus_tag="VFU72_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5423739.1"
                     /translation="MVQETQPQGPEQEQEPEERKVAMVVVAHPDDAEFGSAGTLAKWA
                     KEGWDVYQVIVTDGASGGSDEATEVGPEARRKMTATRKAEQRKAAEVLGVKDVIFLDY
                     PDGEVQPTLELRKDIVRLLRTYRPTRVICPSPDRTWTPAYAIGRYHPDHLAVGQAAMA
                     AVYPASQNPWDFPELLQQGLGPHKIKELYVAGAPVINHAVDISDTIDQKIEALRAHHS
                     QLGDHMEQIEEWIRGGSAKAGEEYNMGYAERFHFSENR"
     gene            complement(2704..>3937)
                     /locus_tag="VFU72_09395"
     CDS             complement(2704..>3937)
                     /locus_tag="VFU72_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5423740.1"
                     /translation="HRPVAPEEALEATRAAWRQWIAGFAYDGPRAPLVRRSTITLKLL
                     NAFASGAMLAAPTSSLPEWIGGPRNWDYRYSWVRDAAHSVYALSRIGMGQEALGFLGW
                     VLDAFERHGRPSVLYTVDGGRPPPEHVDPELRGYRDSPPVRWGNAAADQIQHDIYGEI
                     LDCAYIWQREVGTLDPGLWERLVPLITAARVAWDRPDQGIWEVRTPGRVFTYSAALCQ
                     VALDRGARLALRYRYHGQVERWRSTADEIVGAILRDAWDPKLNALTANLGGGPLDASL
                     LTLPLRRVIPAEHPKMIGTVRAIESGLGAGGGLIYRYLPQELPDGLHSNEGAFLLCSF
                     WLVDNLALQGRPQEAHDLFDKLCDRAGEVGLLPEQIDPTSGAYLGNYPQGFSHVGLIS
                     SGVNLALLAKKQAKAAVP"
BASE COUNT          614 a         1345 c         1390 g          588 t
ORIGIN      
        1 acggaggtct gcccctacca ggatcacgcc cgaagccgtc cgattactca gccccttcag
       61 tgggcttcgt tctgttagcg cggtggtttt caccccgcgc cgggcgcggc cgagcctact
      121 cggtgggcca gcacccgcac cccctcaatc cgccgcgtta tccttcagcc gccgctgcag
      181 cttgatcagc gcctggcgca ggcgcgctag cgagcgctcc ttgcccagca cgcgcagcgt
      241 ctcgaagagg ccgggggaga cggtgcggcc ggtgacggcc acccggatcg gcatgaagag
      301 ctggcccgcc ttgaggtcca gctcgccggc cagggcacgc agccgcacgt cgagcgccgt
      361 ctcgtcgtcg aagtcggcct gctccagcag gcccagcgcg gcctccaacg ctcgcaccac
      421 cagctccggg tcggtcttgc gcgggatcag gaggtccggg tcgtagggct cgggctcggc
      481 ggtgaagaag aaatcggtca gctccactac ctcgggcagc agcttgagcc ggtctttcac
      541 cagcgccacc accgaacgca cgtaggcgtg ctccggcgag cccggctcgc tggcggcggg
      601 gccggccagc cccgcctgct gcaggtaggg cacgcagcgc gcggtcaggt cgtccagcgt
      661 cagcacgtgg ttgatgtagt attggttcat ccaggcgagc ttctcgaagt tgaagcgcgc
      721 cgggctggga ctgacgtccc tcagcgcgaa cttctcgatc agctcctgcc gggtgaagat
      781 ctcctggtcg cccgacgacc agcccaagag cgccaggtag ttgaccagcg cctcgggcag
      841 gtagccggcg tgcttgtact gcagcacgcc cacgtcgccg tcgcgcttgc tcaatttccc
      901 cttgccgtcc ggccgcagga tgaccggcag gtgggcgaag gccggcggct cccagccgaa
      961 cgcctggtag agctgcacgt ggatcggcgc cgaggggatc cactcctcgc cgcgcgtcac
     1021 cgtggtgatc tccatcaggt gatcgtcgac caccaccgcc aggtggtagg tggggtagcc
     1081 gtccgacttg aggatgatcg ggtcctccag cagccggttc tcgaaggcga tctgcccgcg
     1141 caacaggtcg tcgacggcgg tcgtgccgct gtccggcatc ttgaagcgga tggcggtcgg
     1201 cacattccgc gcgcgcagtt cggcgatctc ctgcgcggag aggttgcggc agtggcggtc
     1261 gtagccgggc ggctcgtggc gagcgcgctg ctcgtcgcgc agggcgctca ggcgctcggg
     1321 cgtgcagaag cagtagtagg cgtggccgcg ctcgaccagc tcatgggcgt agtgctggta
     1381 gagctccagc cgctcgctct ggatatacgg tcccttcggc ccgccgacct ccggcccctc
     1441 gtcccagtcc aggcccagcc agcgaaacgc ttccaggatg ccgctcaggc tctgctcgac
     1501 gaagcgcttg cggtcggtgt cctcgatgcg caggatgaag tcgccgtcgt ggtggcgggc
     1561 gtagagccag gtgaagagcg ccgtgcgcgc cccgccgacg tgcagatcgc cggtagggct
     1621 gggcgccatg cgtacccgga cgcgccgctc ggtggtgttc acgcagtgtc tccctcgaaa
     1681 tgcctgctcg tggcgcaccg ataacggtgc tgaacactca agtatgaagc attctccgcc
     1741 gtagggcaag ccgctcgacc agcgcttgcg caaagcgtat gctaggtggt tggccgggca
     1801 tgcgcgccgc ggcgggcggt tggcgtcagc gtcagcgcag gaatggagtc gagattggtt
     1861 caggagactc aaccccaggg gccggagcag gagcaggagc cagaggagcg caaggtcgcg
     1921 atggtggtgg tggcccaccc cgacgacgcc gagttcggca gcgccggcac gctggccaag
     1981 tgggcgaaag agggctggga tgtctaccag gtgatcgtga ccgacggcgc cagcggtggc
     2041 tccgacgagg cgaccgaggt ggggccggag gcgcggcgca agatgacggc gacgcgcaag
     2101 gccgagcagc gcaaggcggc cgaagtcctg ggcgtcaagg acgtcatctt tctcgattat
     2161 cccgatggcg aggtgcagcc gacgctggag ttgcgcaagg acatcgtacg cctgctgcgt
     2221 acctaccgcc cgacgcgagt catctgcccc tcgcccgacc gcacctggac gccggcctac
     2281 gccatcggcc gctaccaccc ggatcacctg gcggtcggcc aggccgccat ggccgcggtc
     2341 tacccggcct cgcagaaccc ctgggacttc cccgagctgc tgcagcaggg gctagggccg
     2401 cacaagatca aggagctcta cgtcgccggc gcgccggtca tcaaccacgc ggtcgacatc
     2461 tccgacacga tcgaccagaa gatcgaggcg ctgcgcgccc accacagcca gctcggcgac
     2521 catatggagc agatcgagga gtggatccgc ggcggctcgg ccaaggcggg cgaggagtac
     2581 aacatgggct acgccgagcg gtttcacttc tcggagaatc ggtaggaggc ggcagagcgg
     2641 cccaacgacc aggcgctgtg ctctcgcgca cggcacccaa tgtcttcgga gccaccgccc
     2701 ccctcacggc actgccgcct tggcctgctt cttggccagc agcgccaggt tgacgccgct
     2761 ggagatcagt ccgacgtggc tgaaaccctg gggatagttg cccaggtagg cgccgctggt
     2821 ggggtcgatc tgctccggta gcaggccgac ctcgcccgcc cggtcgcaga gcttgtcgaa
     2881 gaggtcgtgc gcctcctgcg ggcgcccctg cagcgccaga ttgtccacca gccagaagct
     2941 gcagaggagg aacgccccct cgttgctgtg cagtccgtcg ggcagctcct gcggcaagta
     3001 gcggtagatc aggcccccgc cggctcccag cccgctctcg atcgcgcgca ccgtgccgat
     3061 catcttggga tgctcggcgg ggatcacccg gcgcagcggc agcgtcaaca ggctggcgtc
     3121 gagcggcccg ccgccgagat tggcggtgag cgcgttgagt ttagggtccc aggcgtcgcg
     3181 caggatggcc ccgacgatct cgtcggcggt ggagcgccag cgctccacct ggccgtgata
     3241 gcgatagcgc agcgccagcc gggcgccgcg gtcgagcgcg acctggcaga gcgcggcgga
     3301 gtaggtgaag acgcgccccg gcgtgcgcac ctcccagatg ccctggtcgg gccggtccca
     3361 ggcgacccgc gccgcggtga tcagcggcac caggcgctcc cagagccctg gatcgagcgt
     3421 gccgacttcc cgctgccaga tataggcgca gtcgagaatc tcgccgtaga tgtcgtgctg
     3481 gatctgatcg gcggcggcat ttccccagcg caccggcggc gagtcacgat agccgcgcag
     3541 ctctgggtcg acgtgctcgg gcggtgggcg gccgccgtcg acggtataga gcacgctggg
     3601 gcgcccgtgg cgctcgaacg cgtccagcac ccagccgaga aaaccaagcg cctcctggcc
     3661 catgccgatg cggctgaggg cgtacacgga gtgggccgcg tcgcgtaccc aggagtagcg
     3721 gtagtcccag ttgcgcgggc cgccgatcca ctcggggagc gacgaggtcg gcgccgccag
     3781 catcgcgccc gacgcgaagg cattcagcag cttcagggta atggtcgagc gccgcaccag
     3841 gggggcgcgc ggtccgtcgt aggcgaagcc ggcgatccac tggcgccagg cggcccgcgt
     3901 cgcctccagc gcctcctccg gcgccaccgg acggtgg
//
LOCUS       DASUBI010000489         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_764284, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000489 DASUBI010000000
VERSION     DASUBI010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764284"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFU72_09400"
     CDS             complement(<1..350)
                     /locus_tag="VFU72_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEU5423741.1"
                     /translation="MAPSDIARYRANLQAEIDGTALYRALERLETTPELAGVYRTLAE
                     EEERHADLWRERLKASGATIPTLAPSWRTRALIRLAGWFGPGFILPTVAAHERLDSQR
                     YHEQPEASAAGLPAA"
     gene            582..2339
                     /locus_tag="VFU72_09405"
     CDS             582..2339
                     /locus_tag="VFU72_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423742.1"
                     /translation="MNRVDRFGALYEEWQRHGLDRRQFMRLVALGASASSISMIIAAC
                     GGSSTKSTSTSAPGAPTTSAGGGATATTAGTPQPIAQTVGTPALNPSAMPTSAPFVDK
                     PFLIALNVEPDSLDVHDTFSNASLGTIKCIYEGLVGLNEKMSVVNQLATSWEASSDAK
                     EFTFKLAKGVKFQDGTPFNAQAVKDSFDRVLAKDSKLKRHGYFSAAIDHVEVVDDGTV
                     KIVSQNPFAAMVATIAHPAGGIASPTAIKKYGTDFGTHPVGTGPYQFSEWVRGDHIAM
                     APFADYWNKASGASVSKLTVKGIAEPSALGIAVQSGDAQFGGPLNAPQAVQLQKQQGV
                     VVQENPSISVYWITLNNHIKPYDNKMVRQALNYGVNKEEVLKAADLGKGTLMDSPLGQ
                     LVYGYHKVGSYAYDPAKAKDLLKQGGYENGFKATLWTSAPSKDRAVAVQGQLQKIGVT
                     IDVVQMESAALNAELAKPVDQSQTQMVVSNWSPSTGDADWGLRPLYTKDEWPPAGATY
                     SFYTNPDVEKYVLEGLQFADETKRLEAYAKAQETIWDDAPNIFLYAPTYFGANVQDAM
                     GVIVQPDGIVYMRSAAYKK"
     gene            2375..>2688
                     /locus_tag="VFU72_09410"
     CDS             2375..>2688
                     /locus_tag="VFU72_09410"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5423743.1"
                     /translation="MLRYVIRRLMVTIPVLIGVSIIVFVMMRMVPGDPARQALGPEAT
                     GDEVARLHHEWGLDRPLPIQYVYWANRVIHGDFGRSTVSRVPVTTEIWARFPNTLWLT
                     VA"
BASE COUNT          464 a          957 c          864 g          403 t
ORIGIN      
        1 gccgccggca atccggcggc gctggcctcc ggctgctcat ggtagcgctg gctgtcgagc
       61 cgctcgtgcg cggcgaccgt gggcaggatg aagccggggc cgaaccagcc cgccagccgg
      121 atcagcgcgc gcgtgcgcca ggagggagcc agcgtgggta tcgtcgcgcc gctcgccttc
      181 agccgctcgc gccagagatc cgcgtgccgc tcctcctcct ccgccagcgt gcgatagacc
      241 ccagccagct cgggcgtcgt ctccaggcgc tccagcgcgc ggtagagcgc cgtgccgtcg
      301 atctccgcct ggaggttcgc ccggtagcgt gcgatgtcgc tgggcgccat accctgcctc
      361 cctcccctgt tgggggcgcg accggcaaca gccgggccgg agagcgtgtc gacccgcggg
      421 tccgccgggt cagcgccgat cataccccgg cacgccgccg gccgggacga tcttaacgac
      481 aagacgcctg gctcccaggg ttgtgccctg gtgatagcgt gctatcatca ctccgcagtc
      541 agcccctctt ctggtggttt ctggtggtag gaggagatac gatgaaccgc gtcgatcggt
      601 ttggtgcgct ctacgaggag tggcagcgcc acggcctcga ccgtcgccaa ttcatgcgcc
      661 tggtcgccct cggcgccagt gcgtcctcga tctcgatgat catcgccgcc tgcggcggct
      721 ccagtaccaa gagcacctcg accagcgccc caggtgctcc gaccacgtcg gccggcggtg
      781 gcgcgaccgc gaccacggcc ggcaccccgc agccgatcgc gcagacggtc ggcacccccg
      841 cgctcaaccc ctcggccatg ccgacctcgg cgcccttcgt cgacaagccc ttcctgatcg
      901 cgctcaacgt cgagcccgac tcgctcgacg tgcacgacac cttctccaac gcctcgctcg
      961 gcacgatcaa gtgcatctac gagggcctgg tggggctgaa cgagaagatg agcgtggtca
     1021 accaactggc cacctcgtgg gaggcctcca gcgacgccaa ggagttcacc ttcaagctgg
     1081 ccaagggggt caagttccag gacggaacgc cgttcaacgc gcaggcggtt aaggacagct
     1141 tcgaccgcgt gctggccaaa gactccaagc tgaagcggca cggctacttc agcgcggcca
     1201 tcgaccacgt cgaggtggtg gacgacggca cggtcaagat cgtgtcacag aaccccttcg
     1261 ccgccatggt cgccaccatc gcgcacccgg ccggcggcat cgccagcccc accgccatca
     1321 agaagtacgg caccgacttc ggcacgcacc cggtcggcac cggcccctac cagttctccg
     1381 agtgggtgcg cggcgaccac atcgcgatgg cccccttcgc cgactactgg aacaaggcca
     1441 gcggcgcctc ggtctccaag ctgacggtca agggcatcgc cgagccgagc gcgctcggca
     1501 tcgcggtgca gagcggcgac gcccagttcg gcggcccgct gaacgcgccc caggcggtgc
     1561 agctgcagaa gcagcagggc gtcgtcgtcc aggagaaccc gagcatctcg gtctactgga
     1621 tcacgctcaa caaccacatc aagccgtacg acaacaagat ggtgcgccag gcgctcaact
     1681 acggcgtcaa caaggaagag gtcctcaagg ccgccgactt gggcaagggg acgctgatgg
     1741 actcgccgtt gggccagttg gtctacggct accacaaggt cggctcctac gcgtacgatc
     1801 ccgccaaggc caaggatctg ctcaagcagg gcggctacga gaacggcttc aaggcgacgc
     1861 tctggacctc cgcgccgagc aaggaccgcg cggtggccgt gcaggggcaa ctgcagaaga
     1921 tcggcgtcac tatcgacgtg gtgcagatgg agtcggccgc gctcaacgcc gagctggcca
     1981 agccggtcga tcagtcccag acccagatgg tcgtctccaa ctggtcaccc tcgaccggcg
     2041 atgccgactg gggtctgcgc ccgctctaca ccaaggacga gtggccgccg gccggcgcca
     2101 cctactcctt ctacaccaac ccggacgtcg agaagtacgt gctggagggc ctgcagttcg
     2161 ccgacgaaac caagcgcctg gaggcctatg ccaaggcgca ggagaccatc tgggacgacg
     2221 cgcccaacat cttcctctac gcgccgacct atttcggggc caacgtgcag gacgcgatgg
     2281 gcgtcatcgt gcagccggac ggcatcgtct atatgcggag cgcggcctac aagaagtagg
     2341 ccgccgcacc cgcctggcct gaggagatcg caacatgttg cgctacgtga tccggcgcct
     2401 catggtgacg atcccggtcc tgatcggcgt ctcgatcatc gtcttcgtga tgatgcgcat
     2461 ggtgcctggc gacccggcgc gccaggcact ggggccggag gcgacgggcg acgaggtcgc
     2521 ccgtctgcat catgagtggg gcctggacag gccgctgccc atccagtacg tctactgggc
     2581 caaccgtgtc atccacggcg acttcggccg ctcgaccgtc tcgcgcgtcc cggtcacgac
     2641 cgagatctgg gcacgcttcc ccaacacgct ctggctgacg gtcgccag
//
LOCUS       DASUBI010000490         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_968447, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000490 DASUBI010000000
VERSION     DASUBI010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968447"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFU72_09415"
     CDS             complement(<1..846)
                     /locus_tag="VFU72_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU5423744.1"
                     /translation="MYPAPFDYYRPSQVPEAIALLEQHPEAKLLAGGHSLLPMMKLRL
                     ASPSALIDLHGIPGLAGVQRQGETLIIGPLTTYDALLADPTIQQTLPLMLEAATVVGD
                     LQVRNRGTIGGSAAHADPAADFPAVLLALEAQFRVLGPQGERTIAANDFFQDLFTTAL
                     APNEVLTEIRMPVPAPGTGMAYEKFAHPASGYAIVGVAAVVGPGGQPLRVAITGAGPV
                     ARRASATEQALSGQPLSEQAIARAAEQAGQGMTFLGDIHAGEDYRRHLTQVYTRRALT
                     RAWQQA"
     gene            complement(848..3208)
                     /locus_tag="VFU72_09420"
     CDS             complement(848..3208)
                     /locus_tag="VFU72_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5423745.1"
                     /translation="MVMTRLVGASVKRKEDPRFLTGHGSYTDDVKLPGTLWLEIVRSP
                     HAHAHIKRIDTSQASGMPGVRAVYTGRDLQEKVAPVPCAWLPPNSDLRVPTYRALAVD
                     TARYVGDAVAAVVAETPYQARDAADAVVVEYEPLPVVVDAEQAAQPGAPQLYHDVPNN
                     VAFHWKLYGGDAEAAFKEADVTVSQRLINQRLIPNAMEPRAALASYNPGSGELTVWCT
                     SQNPHIHRLLFSAVTGIHETRIRVISTDVGGGFGSKIPFYAGEMLAATISRELGWPVK
                     WSEDRHENYLATTHGRDHIDYVELAAKRDGTITGVRIKTYANMGAWLSTAAPGVPTWL
                     FGLMACGPYTIPNVAVDVFGTLTNTTATDAYRGAGRPEATYALERTVDLLAKKLEMDP
                     VEIRRRNFIAKDAFPHQTVTGLLYDSGDYDAALDKALDLVGYQDFRRQQAEARQQGKL
                     LGIGFSTYIEVCGLAPSYAAGAMGFQGGLWESATVRVHPSGNVSVFTGSNPHGQGEET
                     TFAQLVSDELGVPLQDIEIVHGDTSRIPFGMGTYGSRSTAVGGTAIVLAARKVRDKAI
                     VLAAHLLEVTPEDIVFDQGNFSVKGAPDRVKTFPEVASAAYLAWSLPPDVHPGLEETH
                     FHDPSNNVFPFGTHICEVEIDPTTGATTFRRYVAVDDVGNVINPMIVDGQVHGGITQG
                     LAQAVYEAAVYDDDGQLLTGTMMDYAVPKATMLPWYEVERTVTPTPVNPMGLKGAGET
                     GTIASTPAVVNAIIDALQPYGIEHLDMPLTPQKIWRAINQQGEGGR"
     gene            complement(3261..3755)
                     /locus_tag="VFU72_09425"
     CDS             complement(3261..3755)
                     /locus_tag="VFU72_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5423746.1"
                     /translation="MASQQIRVTVNGTQHEDAVEPRTLLVSYLRETLNLTGTHVGCDT
                     SQCGACTILLDGRAVKSCTVLAVQADGHELTTIEGLAQNGQLHPIQQGFWEQHGLQCG
                     FCTPGMILTAVDLLQHTPHPTEAEIRHGLEGNLCRCTGYQNIVKAIQQAAEQLQSAPA
                     GDDD"
     gene            complement(3803..4249)
                     /locus_tag="VFU72_09430"
     CDS             complement(3803..4249)
                     /locus_tag="VFU72_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEU5423747.1"
                     /translation="MKISGSQTIQAPRQAVWDRLLDPDSIRHCLPGVEELNQTGDNQY
                     SMTLTVGVGPVRGSYAGAIRMSDVDEPNSYRMEVEGNGRAGFVRGTGTVRLTDDGDSA
                     TTVNYEGDVEVGGPVGAVAQRMLGGVTNRMVGEFFNCIEGQAAPQA"
     gene            complement(4370..>4794)
                     /locus_tag="VFU72_09435"
     CDS             complement(4370..>4794)
                     /locus_tag="VFU72_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423748.1"
                     /translation="VQQYIEPRRETSRRSQLDDREAELRRICLFDVLTNNADRKGSHF
                     FVGEHDGRLWGIDHGLTFNVQPKLRTVIWDYCGEPIPEDLRDDLRRLVRHQRTVQPLL
                     APYLSGSELRVLFRRAADLLLQPVYPHPTHRRSIPYGW"
BASE COUNT          672 a         1591 c         1729 g          802 t
ORIGIN      
        1 cgcctgctgc caggcacggg tcagggcacg gcgggtgtag acctgggtca gatggcgccg
       61 gtagtcctcc ccggcgtgga tgtcgcccag gaaggtcatg ccctgccccg cctgctccgc
      121 cgcacgggca atggcctgct cactgagcgg ctggccactg agcgcctgct cggtggcgct
      181 cgcccgccgg gccaccggtc ccgcgccggt gatcgccacc cgcagcggct gcccccccgg
      241 gccgaccacg gccgccaccc ccacgatggc gtagcccgag gccgggtggg cgaacttctc
      301 gtaggccatg cccgtgcccg gggcgggaac gggcatccga atctcggtca agacttcgtt
      361 gggggcgagc gcggtggtga acagatcctg gaagaagtca ttcgccgcga tggtgcgctc
      421 cccctggggg ccgagcaccc ggaactgagc ctcgagggcc agcagcaccg ccgggaagtc
      481 ggcggccggg tcggcgtggg cggcggaccc gccgatggtg ccgcggttgc gcacctggag
      541 atcgccgacc acggtggccg cctcgagcat gagcggcagc gtctgctgga tggtggggtc
      601 ggccaagagg gcgtcgtagg tggtcagggg accgatgatg agggtctcgc cctggcgctg
      661 caccccggcg agacccggga taccgtgcag gtcgatgagg gcgctggggc tggccaggcg
      721 cagcttcatc atgggcagga gactgtggcc gccggccagg agcttggcct cggggtgctg
      781 ctcaaggagc gcgatcgcct ccggcacctg actcggccgg tagtagtcaa acggcgctgg
      841 gtacatgtta tctccctccc tctccctgct ggttgatcgc tcgccagatc ttctgcggcg
      901 tcagcggcat gtccagatgc tcgatgccgt agggctgcag cgcgtcgatg atggcgttga
      961 ccaccgccgg ggtcgaggcg atggtgccgg tctcgccggc acccttgagg cccatcggat
     1021 tgaccggcgt gggggtcacc gtgcgctcca cctcgtacca ggggagcatg gtcgccttgg
     1081 gcacggcgta gtccatcatg gtgccggtca ggagctgccc gtcgtcgtcg tagaccgccg
     1141 cctcgtagac cgcctgggcc agcccctggg tgatcccgcc atggacctgc ccatcgacga
     1201 tcatcgggtt gatcacgttg ccgacgtcgt ccaccgccac atagcggcgg aaggtggtcg
     1261 cgccggtcgt cgggtcgatc tcgacctcgc agatgtgggt gccgaagggg aagacgttgt
     1321 tcgacgggtc gtggaaatgc gtctcctcca ggccggggtg gacatcgggc ggcagcgacc
     1381 aggccaggta ggccgcgctg gcgacctcgg ggaaggtctt gacccggtcg ggcgcgccct
     1441 tgaccgagaa gttcccctga tcgaagacga tgtcctcggg cgtcacctcc agcaggtggg
     1501 ccgcgagcac gatcgccttg tcgcgcacct tgcgcgcggc cagcacgatc gcggtgccgc
     1561 cgacagcggt gctgcggctg ccgtaggtgc ccatgccgaa ggggatgcgg ctggtatcgc
     1621 cgtgcacgat ctcgatgtcc tgcagcggca cccccagctc atcgctgacg agctgggcga
     1681 aggtcgtctc ctcgccctgg ccgtgcgggt tggagccggt gaagaccgag acgttgccgc
     1741 tggggtggac gcgcacggtg gcgctctccc agagcccgcc ctggaagccc atggctccgg
     1801 cggcgtagga gggcgccagg ccgcagacct cgatgtaggt cgagaagccg atgcccagca
     1861 gcttgccctg ctggcgcgcc tcggcctgct ggcggcggaa atcctggtag ccgaccagat
     1921 cgagcgcctt gtcgagcgcg gcgtcgtagt cgccgctgtc gtagagcagc ccggtgaccg
     1981 tctggtgcgg gaaggcatcc ttggcgatga agttgcgccg ccggatctcg accgggtcca
     2041 tctcgagctt cttggccagc aggtcgacgg tgcgctccag cgcgtaggtc gcctccggac
     2101 ggccggcgcc gcggtaggcg tcggtggcgg tggtgttggt cagcgtgccg aagacgtcga
     2161 cggccacgtt ggggatggtg taggggccgc aggccatcag gccgaagagc caggtcggca
     2221 cccccggcgc ggcggtcgag agccaggcgc ccatattggc gtaggtcttg atgcggacgc
     2281 cggtgatggt gccgtcgcgc ttggcggcca gctcgacgta gtcgatgtgg tcgcgtccat
     2341 gcgtggtggc caggtagttc tcgtgccggt cctccgacca cttgaccggc caaccgagct
     2401 cgcggctgat ggtggcggcc agcatctcgc ccgcgtagaa ggggatcttg ctgccgaagc
     2461 cgccgccgac gtcggtcgag atcacccgga tgcgcgtctc gtggatgccg gtcaccgccg
     2521 agaagaggag ccggtgaatg tgcgggttct ggctggtgca ccagacggtc aactcccccg
     2581 agccggggtt gtagctggcc agcgcggcgc gcggctccat cgcgttgggg atcagccgct
     2641 ggttgatcag ccgctggctg acggtgacgt cggcctcctt gaaggccgcc tcggcgtcgc
     2701 cgccgtacag cttccaatgg aaggcgacgt tgttgggcac gtcgtggtag agctggggcg
     2761 cgcccggctg ggccgcctgc tcggcgtcga ccaccaccgg caacggctcg tactcgacca
     2821 ccaccgcgtc ggccgcgtcg cgcgcctgat agggcgtctc ggccacgacc gccgccaccg
     2881 cgtcgccgac gtagcgcgcg gtgtcgaccg ccagcgcgcg gtaggtgggc accctgaggt
     2941 cggagttggg cggcagccag gcgcagggga ccggcgccac cttctcttgc aggtcgcgcc
     3001 cggtgtagac cgcgcgcacg ccaggcatgc cgctcgcctg gctggtgtcg atgcgcttga
     3061 tgtgggcgtg ggcgtgcggg ctgcgcacga tctccagcca gagcgtgccg gggagcttga
     3121 cgtcgtcggt gtagctgccg tgcccggtga ggaagcgggg atcctccttg cgcttgactg
     3181 atgctccgac gagcctcgtc atgaccatct gggcactcct ttcgggctag actcctccca
     3241 tccggatagc aagggtgcgg ctagtcgtcg tcgccggcgg gggcggactg cagctgctcg
     3301 gccgcctgct ggatcgcctt gacgatgttc tggtagccgg tgcagcggca caggttgccc
     3361 tccaacccgt gccggatctc cgcctccgtc gggtgcggag tgtgctgcag gagatcgacc
     3421 gccgtcagga tcatgcccgg cgtgcagaag ccgcactgca gcccgtgctg ctcccagaag
     3481 ccctgctgga tcggatgcag ctggccgttc tgggccagcc cctcgatggt ggtcagctca
     3541 tgcccgtcgg cctgcaccgc caggaccgtg caggacttga ccgcccggcc gtcgaggagg
     3601 atggtgcagg cgccgcactg gctggtgtcg cagcccacgt gggtgccggt caggttgagg
     3661 gtctcgcgca ggtaggagac cagcagcgtg cgcggctcga ccgcgtcctc gtgctgcgtc
     3721 ccgttgaccg tcacccggat ctgctggctc gccatggttc cctcccgcta tcacctgtgt
     3781 ggtacgaacc tgtctcgtcg atctacgcct ggggcgcggc ctgcccctcg atgcagttga
     3841 agaattcgcc gaccatgcgg ttggtgacgc cgccgagcat gcgctgcgcc accgcgccga
     3901 ccggcccgcc gacctcgacg tcaccctcgt agttcaccgt ggtcgccgag tcgccgtcgt
     3961 ccgtcaggcg aacggtgccg gtgccgcgca cgaacccggc gcggccgttg ccctcgacct
     4021 ccatgcggta gctgttgggc tcgtcgacgt cgctcatgcg aatcgcgccg gcatagctgc
     4081 cgcgcaccgg tcctaccccg accgtcaacg tcatcgaata ttgattgtcc ccggtctggt
     4141 tcaactcttc gactccaggg aggcaatgcc ggatcgaatc cggatcgagc aaccggtccc
     4201 ataccgcctg acgcggagcc tggatcgtct ggctccccga aatcttcatg cagcctcctc
     4261 cgctggtgga gttgaagcga acccgcccgc gaaacgacct gggcaaaacc cggcacatgg
     4321 tagcactgtt tggccgcgcg tcaagcgccc ggccgaagcg gcgcatgtct caccagccgt
     4381 agggaatgct ccgccggtgg gtgggatgag gatagaccgg ctgcaagagc aggtcggccg
     4441 cacggcggaa gaggacgcgc agttcgctgc cggagagata gggagccagc agcggctgca
     4501 ccgtgcgctg gtggcgcacc aggcgacgca ggtcgtcgcg cagatcttcc gggatcggct
     4561 cgccgcagta gtcccagatc acggtgcgga gcttgggctg cacgttgaag gtcagaccgt
     4621 gatcaatgcc ccagagacgg ccgtcgtgct cgccgacgaa gaagtggctt cccttgcggt
     4681 cggcgttgtt ggtcagcacg tcgaagaggc agatgcggcg cagctcggcc tcacggtcgt
     4741 cgagctggct ccggcggctg gtctcgcgcc gcggctcgat atactgctgc accg
//
LOCUS       DASUBI010000491         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_993897, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000491 DASUBI010000000
VERSION     DASUBI010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993897"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VFU72_09440"
     CDS             <1..1492
                     /locus_tag="VFU72_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5423749.1"
                     /translation="SLVRTMIVFFVSFILITVQLVRVQVVDSAAISQRTGRQAGEVTI
                     QDPRLRSAASEVLRGAILTDDGKVVAQTTRLPDGSLLRSYPDPASAYLAGYYSPRLYG
                     SSNLEAAYDDVLMGRKGGNPVTQWLDGLLHRTKKGYNLTLTIDDDLQKQADQLLGDRQ
                     GAVVLMDAKTGAVRAFVSKPNFDPNKLYVNQGPGLDQQVGTATAYWQQLNGAPGSPLL
                     LKPTQGLFIPGSIFKTVTASAAIETGTAKPDTVYRDEGQLAVESRVIPEENRPDPNRI
                     DYTLSDAYAYSLNVVFAQVGLQLGPENLAEYAHRFGFGQMIPFTLPVVPSQLTTDPNF
                     LSSPAALAVTAFGQGQLQVTPLQMALVAQAVANNGEMLTPYLVEKTSTQDGKTLDERE
                     PTVWQRAITADTAATMRQIMVHSVESGYASPAKIPGYVVGGKTGTAEVGANQQPHAWF
                     IGFAGKDVNNPQYVVAVLVQNGGEGVQQALPIGRDLLQAALTRQQP"
     gene            1926..2912
                     /locus_tag="VFU72_09445"
     CDS             1926..2912
                     /locus_tag="VFU72_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423750.1"
                     /translation="MRGEPARTASHDGPEQPAGIIDTIGQAFALLSGRPYLIWPPVLL
                     DLFLWLGVHLSAQPLTDILQQWIRGSSAGNDTTLRQLQNLGLHYNVFELVGVAMPSLV
                     QGMGTEPTAGVTPTWIGGLPWGWLALIAAGLLAVGAVVGMAYLTIIGHLVGLEAIRPE
                     PFVRAAARNVGHLIGFVLVTIGIVILLALPVLTLTIALLVLGINVLPFAEFVMTVGVL
                     CLFLLLYFGQDAIVVSDAGPMRAVYLSYNVVRRNFWPTVGFALISMIVGTGVPLAMSI
                     FTHSPWSMPFALVGHAFVATGMLAASMLFYRERARALLATPVSVPASESRLS"
     gene            2927..>3787
                     /locus_tag="VFU72_09450"
     CDS             2927..>3787
                     /locus_tag="VFU72_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5423751.1"
                     /translation="MADTTLTHYTAADIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIH
                     EIVDNSVDEAIAGFCDKIIVTIHADGAVTVEDNGRGIPIDLHPKLGKSALEVIMTVLH
                     AGGKFGGGGYKVSGGLHGVGASVVNALSEWVEVEVSKDGSLYRQRYERGVPQGDVERV
                     GDAEPGRCGTTTRFLPDPQIFDTLDYSFDILLQRFREMCYLTRGLGITFRDERDDREM
                     SFYFDGGIISFVRHLNRNKNVLHPKPFYVLREVNDCMVEVALQYNDGYAESTHTFANN
                     INTVDGGTHLT"
BASE COUNT          652 a         1233 c         1248 g          654 t
ORIGIN      
        1 ctcgctggtg cgcaccatga tcgtcttctt cgtctcgttc atcctgatca cggtgcaact
       61 ggtgcgggtc caggtcgtcg atagtgcggc gatcagccag cgcaccggcc ggcaggcggg
      121 cgaagtgacg atccaggacc cgcggctgcg ctcggcggcg agcgaggtgc tccgtggcgc
      181 catcctcacc gacgacggca aagtcgtggc gcaaacgacc cggctgccgg atgggagcct
      241 gctgcgcagc tatcccgatc cggccagcgc ctacctggcc ggctactact cgccgcggct
      301 gtacggcagc agcaacctcg aagcggctta cgacgacgtg ttgatgggcc gcaaaggcgg
      361 caacccggtg acgcagtggc tcgacgggct gctgcaccgc accaagaaag gctacaacct
      421 gaccctgacg atcgacgacg atctgcagaa gcaggcggat caactgttgg gcgatcgcca
      481 gggcgcggtc gtgctgatgg atgccaagac cggcgcggtg cgagccttcg tcagtaagcc
      541 gaacttcgac cccaacaagc tctacgtcaa ccagggtccg gggctcgacc agcaagtggg
      601 cacggcgacc gcctactggc aacagttgaa cggcgcgccc ggctcgccgc tgctgctcaa
      661 gccgacgcag ggcctcttta tccccggttc gatcttcaag accgtgacgg cctccgccgc
      721 gatcgagacg ggcaccgcca agcccgacac ggtctaccgc gacgagggtc agctggcggt
      781 cgagtcgcgc gtgatcccgg aggagaaccg gcccgaccca aaccgaatcg actacactct
      841 gagcgatgcc tacgcctact cgctgaatgt cgtcttcgcg caagtgggtc ttcaactcgg
      901 cccggagaat ctggccgagt acgcgcaccg cttcggcttt ggccagatga tcccattcac
      961 gctgccggtg gtccccagcc agttgaccac cgatccgaat ttcctgagta gcccggccgc
     1021 gctggcggtg accgccttcg gccagggaca gctccaggtg acgccgctgc agatggcgct
     1081 ggtggcgcag gcggtggcca acaacggcga gatgctgacg ccgtatctgg tggagaagac
     1141 cagcacgcag gacggcaaga cgctggatga acgcgagccg accgtctggc agcgcgcgat
     1201 cacggcggac acggcggcga ccatgcggca gatcatggtg cactcggtcg agagcggcta
     1261 cgcctcgccg gccaagattc ccggctacgt ggtcggcggt aagaccggca cggccgaggt
     1321 cggtgcaaac cagcagccgc acgcgtggtt catcggcttc gcgggcaaag acgtgaacaa
     1381 cccgcagtat gtggtcgccg tactggttca aaatggcggc gagggtgtcc agcaggcgct
     1441 tccgatcggc cgtgacctgc tgcaagcggc gctgacgcgc cagcagccgt aagagacgcg
     1501 ggcggtggct gtcgaagacg ttgggcgctg agtgaggacg gatccttcga caagctcagg
     1561 ctgagcgagt ctggtgagtc gccttcctgg aaggatcgcc gtactcgtac gtcgcttgct
     1621 ccttggtcat cccgagtgga gtcgagggat ccgtgcgccg cgcggcaccc accgaattca
     1681 gcggcattct gcccccaatc cggcacgcgc tcgctattgt ccgtacgtcg ccgtgcgctc
     1741 caattttggc gtcaggacgc ggattctggt aaaattgtgt catgttcggc gcgtgggacc
     1801 gggcgtctcg cgagcggtgc attgcgcgat tgcatggacc gcgaagcagc ggctgtgcct
     1861 cgcgaacggc gacctggagc cgggcggatg agatcgcgcg gagacccggc acgaaacgga
     1921 ggcgcatgcg cggcgaaccg gccaggacag caagccacga tggaccggaa cagccggccg
     1981 gcatcatcga caccatcggt caggcgttcg ccttgctcag cggtcgcccg tacctgatct
     2041 ggccccccgt gttgctcgac ctatttctct ggctcggcgt gcacctttcg gcccagccgt
     2101 tgaccgatat cctgcagcag tggattcgcg gctccagcgc cggcaacgac acgacgctgc
     2161 ggcagctgca gaatctcgga ctccactaca acgtgttcga actggtcggg gtagcgatgc
     2221 ccagcctggt gcaggggatg gggacggaac caaccgccgg ggtcaccccc acctggatcg
     2281 ggggactacc ctggggctgg ctggcactca tcgcggccgg gctcttggcc gtgggcgcgg
     2341 tggtagggat ggcctacttg accatcatcg gccacctggt gggtttggag gccatacgcc
     2401 ccgagccgtt cgtgcgcgcg gccgcgcgca atgtcgggca tctgatcggg ttcgtactgg
     2461 tgacgatcgg catcgtgatc ctgctggcgc tgccggtgtt gacgctcacc atcgcgttgc
     2521 tggttctcgg catcaacgtg ctgccgtttg ccgagttcgt gatgacggtg ggcgtgctct
     2581 gcctgttctt gctgctctat ttcgggcagg atgccatcgt ggtgtcggac gccgggccga
     2641 tgcgcgccgt gtacctgagc tacaacgtcg tgcggcgcaa tttctggccg accgtcggct
     2701 tcgccctgat cagcatgatc gtgggcacgg gcgtcccctt ggcgatgagc atcttcacgc
     2761 attcgccctg gagcatgccg tttgcgctag tcgggcacgc gttcgtcgcg accggcatgc
     2821 tcgccgcctc catgctgttc tatcgggagc gcgctcgcgc actcctcgcg acccctgtat
     2881 ccgtcccggc atccgaaagc cgactcagct gaaaggccct gaactcgtgg cagacaccac
     2941 gcttacgcac tacaccgccg ccgatattca agtgctggag ggactggaag ccgtccgccg
     3001 ccggccgggc atgtacatcg gcagcaccga tattcgcggc ctgcaccacc tgattcacga
     3061 gatcgtggac aacagcgtcg atgaggcgat cgccggcttc tgcgacaaga tcatcgttac
     3121 gatccacgcc gacggcgccg tgacggtcga ggacaacggt cgcggtatcc cgatcgacct
     3181 gcacccgaag ctgggcaagt cggcgctgga ggtcatcatg acggtgctgc acgccggcgg
     3241 caagttcggc gggggcggct acaaggtctc cggcgggctg cacggcgtgg gcgcttcggt
     3301 ggtcaacgcg ctctccgagt gggtggaggt cgaggtcagc aaggacggct cgctctaccg
     3361 ccaacgctac gagcggggcg tgccccaggg cgacgtcgag cgcgtgggcg acgccgagcc
     3421 gggtcgctgc ggcaccacca cgcgtttcct gccggatccg cagatcttcg acacgctcga
     3481 ctatagcttc gacatcttgc tgcagcgctt ccgcgagatg tgctatctga cgcgcggact
     3541 cggtatcacg ttccgcgacg agcgcgacga ccgcgaaatg tcgttctact tcgacggcgg
     3601 cattatcagc ttcgtgcgcc acctcaatcg caacaagaac gtgctgcatc ccaagccgtt
     3661 ctacgtgctg cgcgaggtca acgactgcat ggtcgaggtc gcgctgcagt acaacgacgg
     3721 ctacgccgag tcgacgcata cgttcgccaa caatatcaac accgtcgacg gcggcacgca
     3781 cctgacc
//
LOCUS       DASUBI010000492         6202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_888374, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000492 DASUBI010000000
VERSION     DASUBI010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888374"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..932
                     /locus_tag="VFU72_09455"
     CDS             153..932
                     /locus_tag="VFU72_09455"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU5423752.1"
                     /translation="MPVPDETRTAGQSPATSGAATTDQSIPMRIIVTENGPYQVEGSI
                     PLMQQIIETDEEGASWEWREGTVFRSRRRYRLCRCGQSQNKPFCDGSHETNGFIGTET
                     ASRAPYLQQAKAYQGPELTLTDAEPLCASARFCDACGSIWNLVEEAGAEARALTIREA
                     AHCPSGRLVVWKRAADGKMELAGEPEFEPSIGVVEDPPAGVSGPLWVRGGIEIVSADG
                     TPYEIRNRVTLCRCGQSRNKPFCDGSHRVVDFQDELPLGPE"
     gene            complement(1089..2135)
                     /locus_tag="VFU72_09460"
     CDS             complement(1089..2135)
                     /locus_tag="VFU72_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU5423753.1"
                     /translation="MSGSLTWYVARGSGIAAYLLLTLSVAAGIALSRRWHGRTLPRLV
                     VDGWHRWLTLSFYLFVALHVVTLLLDPFTKFQAPDLLVPFFSDYRPLWTSLGIIAAEL
                     GLALGASVLVRRWIGYRAWHILHGLTYLLFPLSLLHGLGDGSDSRTVWALALYAGSAL
                     LVLAAVAWRAADSPRWRLPAVTAGAVVTVAILLWSLVGPLAPGWAKAAGTPARLLDQA
                     HEAQPASVIPADQSRVAALPAGLSEPIAGQVLLLNNGSQVLLRGSTGSGSRPLDLALQ
                     IDNRDAGGSGEVQLRTSDQTPVCAGPIGGNRDRVLTATCSGYGQSYSLRITLSQVDIG
                     GFSGTLTVLGGPHV"
     gene            complement(2132..3079)
                     /locus_tag="VFU72_09465"
     CDS             complement(2132..3079)
                     /locus_tag="VFU72_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5423754.1"
                     /translation="MLAEMHEIESPAFGEFQALGSTIVVGVTAPDRLERVLAHVRARV
                     NEIDRTLSRFRPDSELRRLEATPGKPRQVTPLFMEALDRSIQAARSTGGLFDPTVRDA
                     VEAAGYDRSIERLEAEGPGPARPTAPAGGWERIQYDPAWNIVTLPEGVRLDFGGIGKG
                     LAVDYALRGLQREPFGVLVSAGGDLAVAGPAPGEGWLCGISVTADAPLADTVLLSAGA
                     LATSGLGRRQWRRDGRRLHHLIDPRSGLPAESPWQIVTVAAGSCVVAEVLAKAAWLLG
                     EAGPDWLARHGFAGRFQDQQSGVVTVGAWPARVNDGDGA"
     gene            complement(3089..3592)
                     /locus_tag="VFU72_09470"
     CDS             complement(3089..3592)
                     /locus_tag="VFU72_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423755.1"
                     /translation="MTIDDHEETRPPEAAEARRAALGRQLRSLRWGAVVLGAAATAAF
                     SGLALRQSSATAGTPAATVQQSQTSGAAAPSQSLFGMGTGSGSSAATQPGASATEGDG
                     FGRDGRFRHRSSFGSSGGDDGSASSSDPFGGDSSSSSSSSSGGTSLAPAPAPSLGGRV
                     HTRSASS"
     gene            complement(3579..3845)
                     /locus_tag="VFU72_09475"
     CDS             complement(3579..3845)
                     /locus_tag="VFU72_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5423756.1"
                     /translation="MAPKVRLRVDPTRCTAFGFCAEFFPEAFALDDWGYAWLQARELP
                     PEQLALARETARLCPTGAITVEIVEQPEPEPIRRLVGKHGDDHR"
     gene            complement(3835..5139)
                     /locus_tag="VFU72_09480"
     CDS             complement(3835..5139)
                     /locus_tag="VFU72_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5423757.1"
                     /translation="MTLAGTESRLALARLLPPDVPALESLADYRAGGGYAAAVWDRAS
                     SELLAEIEASGLRGRGGSAFPSGRKWRAVAEQRGKKVVLVNAAESEPASRKDRTLLAR
                     RPHLVIEGALLATRAVGAGELVVYLHDDDRELRATVEDAFAALRRARFALPRWRVVTA
                     PPGYVAGEESAAVRRVNGGPAKPTAKPPRPFERGVRGRATLVNNVETLANVPLIARHG
                     PEWFRAAGTTDLPGTLLVTLSGAVRRPGVYEVSGGVALGAIIAELGGGVSEGGGIQAL
                     LAGGYFSGWLPGETAAGVALEPETLTRRGVALGSGAIVVVPDSVCGLAQAARLLRFFA
                     DESARQCGPCTYGTPAMAEVFAHLRDGTATARELERLRLWSERMLPGRGACGHLDGAA
                     WAARSALTVFAGEIATHARFGGCGRTRRVMLPGLDEGAHHGA"
     gene            5283..>6202
                     /locus_tag="VFU72_09485"
     CDS             5283..>6202
                     /locus_tag="VFU72_09485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5423758.1"
                     /translation="MSTQTKGAGVKITIIGAGAIGGTAGAFLTQAGYDVTLVDVVEEH
                     VRAMQQRGLRITGARGDQTYAVRALTPDELAGPLQTVILAVKAHFTEAAMTQYAPLLA
                     PDGYVVSFQNGLNEEIIARHVGAERTIGAFIHFGADYLEPGLIRLAWEQPIHLGELNG
                     EITPRLETLQEVLSHVMPTELTTNIWGYLWGKLVYGTMAYTVSIVDAPVPEVLANPLA
                     RAVARAAAAETARIAAAQGYHLERIGGFDPTAFLPAAGWATHADTQLAIVAQEMSGEK
                     QHMGHWRDLKVKKRKTDVDRQQEIIARGREL"
BASE COUNT          973 a         2278 c         2194 g          757 t
ORIGIN      
        1 agtagccgcg actggcggaa cacagcggcg aacagggcta ttcacgcagc ggtaactagg
       61 gtaggatgtg gaattatcac acaggcttgg gcgggcgcgg ccgatcgggc ccgctctacc
      121 cctcacagac ccacacggga gaggagttct ccatgccagt acccgatgag acgcggacgg
      181 cgggccaatc accggccaca agcggcgcgg ccacgaccga tcagtccatc cccatgcgga
      241 tcatcgtcac cgaaaacggg ccataccagg tcgaaggcag catcccgctc atgcagcaga
      301 tcatcgagac cgacgaggag ggagcttcct gggagtggcg cgagggcacc gtcttccgct
      361 cacggcggcg ctaccgcctc tgccgctgcg gccaatcgca gaacaagccc ttctgcgacg
      421 gctcgcacga gaccaacggc ttcatcggga ccgagaccgc ctcgcgcgcg ccctacctgc
      481 agcaggccaa ggcctatcaa ggccccgagc tgacgctgac cgacgccgag ccgctctgcg
      541 cgtccgcccg cttctgcgac gcctgcggca gcatctggaa cctggtcgag gaggcgggcg
      601 ccgaggcccg cgcgttgacg atccgcgagg cggcccactg cccctcgggg cgactggtcg
      661 tctggaagcg cgcggcggac ggcaagatgg agctggccgg cgaaccggag ttcgagccgt
      721 cgatcggcgt ggtcgaggat cccccggcgg gcgtcagcgg tcccctgtgg gtgcgcggcg
      781 gcatcgagat cgtctccgcc gacggcaccc cctacgagat tcgcaaccgc gtcacgctct
      841 gccgctgcgg ccaatcccgg aacaagccgt tctgcgacgg ctcgcaccgc gtcgtcgact
      901 tccaggacga gctgccgctc ggccccgagt gaggctcgcg gcgggcgaca acgcccggcg
      961 ctgtgtgaag gagggcagac acggaggtct gcccctacca catagtcgaa acgcgctaac
     1021 cctgtagggg cggacctctg tgtccgccct ttctccccga cgccttgttt ccgggttaga
     1081 ccacgaccct acacatgcgg gccgccgagg accgtcagcg tgccgctgaa gccgccgatg
     1141 tcgacctggc tcagggtgat ccgcaggctg tagctctggc cgtagccgga gcaggtcgcg
     1201 gtcagcaccc ggtcgcggtt gccgccgatc ggtccggcgc agaccggcgt ctggtccgag
     1261 gtgcgcagtt gcacttcacc gctgccgccc gcgtcgcgat tgtcgatctg cagcgcgagg
     1321 tcgagcggcc gcgatcccga gccggtgctg ccgcgcaaga gcacctggct gccgttgttc
     1381 agcaggagca cctgcccggc gatcggctcg ctgagtccgg cgggcagggc ggccacgcgc
     1441 gactgatcgg ccgggatgac cgaggcgggc tgcgcctcat gcgcctggtc gagcaggcgc
     1501 gcgggcgtgc cggcggcctt cgcccagccg ggcgccagcg ggccaaccag cgaccagagc
     1561 aagatggcca ccgtgaccac cgcaccggcc gtgaccgccg gcaggcgcca gcgcggcgaa
     1621 tccgccgccc gccaggcgac cgcggcgagc accagcagcg cgctccccgc gtagagggcc
     1681 agcgcccaga cggtccggct gtcgctgccg tcgccgagcc cgtgcagcag cgagaggggg
     1741 aagaggagat aggtcaggcc gtggaggatg tgccaggcgc ggtagccgat ccagcggcgc
     1801 acgaggacgc tggccccgag cgccaggccc agctccgccg cgatgatccc gaggctggtc
     1861 cagagcggac gatagtcgct gaagaagggc accagcaggt cgggcgcctg gaacttggtg
     1921 aaggggtcga gcagcagcgt caccacgtgc agggccacga agaggtagaa gctcagcgtc
     1981 agccagcggt gccagccgtc gaccaccagc cgcgggagtg tccggccgtg ccagcgacga
     2041 ctgagcgcga tcccggcggc caccgacagc gtcaggagca ggtaggcggc gatcccgctg
     2101 ccgcgcgcga cgtaccaggt caggctgccg ctcatgcccc atccccgtcg ttcacgcggg
     2161 cgggccaggc gcccacggtc accacgccac tttgctgatc ttggaaacgt ccggcgaagc
     2221 cgtgccgggc gagccagtcc gggccggcct cgcccagcag ccaggcggcc ttggccagca
     2281 cttcggccac cacgcagctc ccggcggcga ccgtcacgat ctgccagggg ctctcggccg
     2341 gaaggccgct gcgcgggtcg atcaggtggt gcaggcgccg gccgtcgcgc cgccactggc
     2401 ggcgaccgag gccggaggtc gccagcgcgc cggccgagag gaggacggta tcggccagcg
     2461 gcgcgtcggc cgtgaccgag atgccgcaca gccagccctc gcccggcgcc ggaccggcga
     2521 ccgccagatc gcccccggcg ctgaccagca cgccgaacgg ctcgcgctgc aggccgcgca
     2581 gggcgtagtc gaccgccagc cccttgccga tgccgccgaa gtcgagccgc acgccctcgg
     2641 gcagcgtcac gatgttccag gcggggtcgt actggatgcg ctcccagccg ccggccggcg
     2701 ccgttgggcg ggccggaccc ggcccctcgg cctccagccg ctcgatgctg cggtcgtagc
     2761 ccgccgcctc gaccgcgtcg cgcacggtcg gatcgaagag gccgccggtc gacctggccg
     2821 cttgaatcga gcggtcgagc gcctccatga agaggggcgt cacctgccgc ggcttccccg
     2881 gcgtggcctc cagccggcgc agctcgctgt cgggccggaa acggctcagc gtgcgatcga
     2941 tctcgttgac gcgcgcgcgc acgtgcgcca gcacgcgctc cagccggtcg ggcgcggtca
     3001 cgccgacaac gatggtgctc cccaaagcct gaaactcgcc gaaggccggc gactcgatct
     3061 catgcatctc ggcaagcata gcagccacct atgaggaagc tgagcgggta tgcacccggc
     3121 cacccagcga cggagcggga gcgggcgcca ggctggttcc gccggaggac gaactggagc
     3181 tggacgaact gtcgccgccg aacggatcgg acgacgaggc cgagccgtca tcaccgcccg
     3241 agctgccaaa ggacgagcgg tggcggaagc ggccatcgcg gccgaagccg tcgccctccg
     3301 tcgcggacgc gcccggctgc gtggcggcgc tggagccgga gcccgtgccc atgccgaaga
     3361 gcgattggct gggcgcggcg gccccgctcg tctggctctg ctggaccgtg gcggcgggcg
     3421 tacccgcggt ggcgctgctc tgccgcagcg ccaggccgga gaaggcggcg gtggcggcgg
     3481 cgccgagcac cacggcgccc cagcgcagcg agcgcaactg gcggccgagc gcggcgcggc
     3541 gcgcttcggc ggcttcaggc ggccgcgtct cttcgtgatc atctatggtc atcgccgtgc
     3601 ttcccaacta gccgccggat cggttccggc tcgggctgtt ccacaatctc aaccgtgatg
     3661 gcgccggtcg ggcagaggcg cgccgtctcg cgcgccagcg ccaactgctc cggcggcagc
     3721 tcgcgcgcct gcagccaggc gtagccccag tcgtccagcg cgaacgcctc ggggaagaac
     3781 tcggcgcaga agccgaaagc ggtgcagcgc gtcggatcga cgcgcaggcg caccttaggc
     3841 gccatggtgc gctccctcgt ccagccccgg cagcatcacc cggcgcgtcc gcccgcagcc
     3901 gccaaagcga gcgtgggtgg cgatctcccc cgcgaacacc gtcagcgctg agcgcgccgc
     3961 ccaggccgcg ccgtcgaggt ggccgcaggc gccgcgaccg ggaagcatgc gctcgctcca
     4021 gagccgcagc cgctccagtt ctcgcgccgt cgccgtgccg tcgcgcaggt gggcgaagac
     4081 ctcggccatg gccggcgtgc cgtaggtaca ggggccgcac tggcgcgccg actcgtcggc
     4141 gaagaagcgc agcaggcgcg cggcctgcgc cagcccgcag accgagtcgg gcacgaccac
     4201 gatggcgccc gagccgagcg ccacgccgcg gcgcgtcagc gtctccggct ccagcgccac
     4261 cccagccgcc gtctcgcccg gcagccagcc ggagaagtag ccgccggcca gcagcgcctg
     4321 gatgccgccg ccctcactca ccccgccgcc caattcagcg atgatcgcgc ccagcgccac
     4381 gccgcccgac acttcataga cgccgggtcg ccgcacggca ccggagagcg tcaccagcag
     4441 cgtgcccgga agatccgtcg tgccggccgc gcggaaccac tccggtccgt ggcgcgcgat
     4501 cagcggcaca ttggccaggg tctcgacgtt gttgaccagc gtggcccggc cgcgcacgcc
     4561 acgctcgaac gggcgtggcg gcttggcggt cggtttggcc gggccgccat tgacgcgacg
     4621 gaccgcggcc gactcttcac cggccacata gccgggcggc gccgtcacca cgcgccagcg
     4681 cggcagcgcg aagcgggcgc ggcgcagcgc ggcgaaggcg tcctcgaccg tcgcgcgcaa
     4741 ctcccgatcg tcgtcgtgca ggtagacgac cagctccccc gccccgacgg cacgggtggc
     4801 gagcagggcg ccctcgatga ccaggtgtgg ccgccgcgcg agcagcgtgc ggtccttgcg
     4861 gctggccggc tccgactccg ccgcgttgac cagcacgacc ttcttgccgc gctgctccgc
     4921 caccgcgcgc cacttgcgcc cgctggggaa ggccgagccg ccacgcccgc gcagcccgct
     4981 ggcctcgatc tcggccagca gttcggagga cgcgcggtcc cagaccgccg ccgcgtagcc
     5041 gccacccgcg cggtaatccg ccagggactc cagcgcgggt acatccggcg gcagcagccg
     5101 cgccagcgcg agcctcgatt ccgttcctgc cagtgtcacc gtcacctcag accagaacca
     5161 accaatagtg ccgggttctc ccgatctgta ccagatgata gacagcccgg ctaaacgctt
     5221 cgtgaaaagg gtatgagaga atcataagaa acacgccagg ggacgtggga atgggagtgg
     5281 gtatgagtac gcagacgaag ggagcagggg tgaagatcac catcatcggc gccggggcga
     5341 tcgggggcac ggcgggcgcg tttctgaccc aggcaggcta cgacgtgacg ctggtcgacg
     5401 tggtcgagga gcacgtgcgc gccatgcagc agcgggggct gcgcatcacc ggggcgcgcg
     5461 gcgaccagac ctatgcggtg cgcgcgctga cgccggatga gctcgccggc ccgctgcaga
     5521 cggtgatcct ggcggtcaag gcgcacttca ccgaggcggc gatgacccag tacgcgccgc
     5581 tgctggcgcc ggacggctac gtcgtctcct ttcagaacgg gctcaacgag gagatcatcg
     5641 cgcgccacgt cggcgccgag cgcaccatcg gcgccttcat ccacttcggc gccgactacc
     5701 tggagccggg cctgatccgg ctcgcctggg agcagccgat ccacctgggc gagctgaacg
     5761 gggagatcac gccgcggctg gagacgctgc aagaggtgct cagccacgtc atgccgaccg
     5821 agctgacgac caatatctgg ggctacctct ggggcaagct ggtctacggc accatggcct
     5881 acaccgtctc gatcgtcgac gcgccggtgc cggaggtgct ggccaacccg ctggcccgcg
     5941 cggtggcccg cgccgccgcc gccgagaccg cgcgcatcgc cgccgcgcag ggctaccacc
     6001 tggagcgcat cggcggcttc gacccgaccg ccttcttgcc cgccgccggc tgggccaccc
     6061 acgccgacac ccagctcgcc atcgtggccc aggagatgag cggcgagaag cagcacatgg
     6121 gccactggcg cgatctgaag gtcaagaagc gcaagaccga cgtcgaccgc cagcaggaga
     6181 tcatcgcccg tggccgggag ct
//
LOCUS       DASUBI010000493         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_546180, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000493 DASUBI010000000
VERSION     DASUBI010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546180"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFU72_09490"
     CDS             complement(<1..1069)
                     /locus_tag="VFU72_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5423759.1"
                     /translation="MFFEQIVRQDIGCAAYIVGSNDAGAAAVVDPRIDMTGEILELLA
                     REGLRLRYIVETHNHADHVAGHHELRARTGAEIAVHAAAGVDYPHRALRHGDELALGE
                     VRLRVIHTPGHRPEHIALAVLDTSRAAEPWLVLTGDSLFIGDVARPDLAIDGREGAGA
                     LFHSLHQGLLELPEGTLVFPGHVAGSLCGRVNNRMTGTTIGFERRHNPALTITDEPAF
                     VAYMTQSLPQRPPNMARIVRLNQSAEPLAQPALRLLSLDQLEPLRQDGATLLDVRSAA
                     AFAAGHLPGSFSVDLHGGQFQNRVGLAVPLDRPLVLVAETEADAEAAARALAVIGVER
                     IAGGLVGPPETASLGLTPEITA"
     gene            complement(1082..1480)
                     /locus_tag="VFU72_09495"
     CDS             complement(1082..1480)
                     /locus_tag="VFU72_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5423760.1"
                     /translation="MSQDYGFGVNLDASYEEAVVRTKEALKTQGFGVLSEIDVRQTLH
                     DKLGVEMEPYVILGACNPPLAHRALETEPEIGLLLPCNVVVRARGAGSRVDIADPQAM
                     LGIAAADQLDEVAQEAKRRLQLVAQALAEQ"
     gene            complement(1477..1911)
                     /locus_tag="VFU72_09500"
     CDS             complement(1477..1911)
                     /locus_tag="VFU72_09500"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5423761.1"
                     /translation="MNRERELQLYRMQAELCQILADPTRLELLHALESGPKRVKDLME
                     ITSQRQAKISQHLAILRQRGIVEGQRAGNEMHYALRDRRIIDACRITREILLDQLSGQ
                     AALVRAVRDEPAASAATPPQLRSDDVDDPRYSAARGREGDRP"
BASE COUNT          319 a          803 c          826 g          375 t
ORIGIN      
        1 cggccgtgat ctccggcgtc agtccgagcg aggccgtctc gggcgggccg accaggccac
       61 cggcgatccg ctccacgccg atcaccgcca gcgcgcgcgc cgccgcctcg gcatccgcct
      121 ccgtctccgc caccaggacc agcggacggt cgagcggcac ggccaggccg acccggttct
      181 ggaactgtcc gccgtgcaga tcgaccgaga acgaacccgg caggtgcccg gcggcgaagg
      241 cggccgcgga gcgcacatcg agcagggtgg cgccgtcctg ccgcaggggt tcgagctggt
      301 ccaggctcag caggcggagc gccggctgcg cgagcggctc ggcgctctgg ttcagccgga
      361 cgatgcgcgc catgttcggc ggtcgctgcg gcaggctctg ggtcatgtag gcgacgaagg
      421 cgggctcgtc ggtgatggtc aaggccgggt tgtggcgccg ctcgaagccg atggtcgtac
      481 cggtcatgcg gttgttgacg cggccgcaga gcgagccggc gacgtgcccg ggaaagacca
      541 gcgtcccctc gggcagctcc aggagaccct ggtgcaggct atggaagagc gcgccggcgc
      601 cctcccggcc gtcgatcgcc agatccgggc gggccacgtc gccgatgaag agcgagtcgc
      661 ccgtgagcac gagccacggc tcggcggcgc gcgaggtgtc gagcaccgcc agcgcgatgt
      721 gctccgggcg gtggccgggc gtgtggatga cgcgcagccg gacctcgccc agcgccagct
      781 cgtcgccgtg acgcagggcg cgatggggat agtcgacacc cgccgctgcg tggacggcga
      841 tctcggcgcc ggtgcgcgcg cgcaattcat gatggccagc gacgtggtcg gcgtggttgt
      901 gcgtctccac gatgtagcgg agccgcaggc cctcgcgggc cagcaactcc aggatctcgc
      961 cggtcatatc gatgcgcgga tcgaccacgg ccgccgcacc ggcgtcgttg gagccgacga
     1021 tataggcggc acagccgata tcctgacgta cgatctgctc gaagaacacg cgcgttgctc
     1081 cttactgctc ggccagcgcc tgcgccacca actgcaggcg gcgcttggcc tcctgggcca
     1141 cctcgtcgag ctggtcggcc gcggcgatgc cgagcatcgc ctgcggatcg gcgatgtcga
     1201 cgcggctgcc ggcgccgcgg gcgcgcacca ccacgttgca gggcaggagc aggccgatct
     1261 ccggctccgt ctccagcgcc cggtgcgcca gcggcgggtt gcaggcgccc agaatgacgt
     1321 agggctccat ctccacgccg agcttgtcgt gcagcgtctg gcgcacgtcg atctcgctca
     1381 gcacgccgaa cccctgggtc ttcagcgcct ccttggtgcg tacgacggct tcctcatacg
     1441 aggcatcgag gttcacgccg aacccgtagt cctggctcat ggtctgtctc cttcgcggcc
     1501 gcgcgccgcg gaatatcgcg gatcgtccac atcgtctgag cgcagctgcg gcggcgtcgc
     1561 cgcgctggcc gcgggctcat ccctcacggc tcgcaccagc gccgcctggc cgctgagctg
     1621 gtcgagcagg atctcgcgcg tgatccggca ggcgtcgatg atgcgccggt cgcgcagcgc
     1681 gtagtgcatc tcgttgccgg cgcgctggcc ctcgacgatc ccgcgctggc gcaggatcgc
     1741 caggtgctgg ctgatcttgg cctggcgctg gctggtgatc tccatgagat ccttgacgcg
     1801 cttgggtccg ctctccagcg cgtgcagcag ctccagacgc gtcggatcgg ccaggatctg
     1861 acacagctcg gcctgcatcc ggtagagctg gagttcccgt tctcgattca cctccaatgt
     1921 cccgctccga tccttcattc gaatatccga atatagccag agtatagggg tgatcgcggc
     1981 gggagtcaag aggcggcgcc gtcgcggcgg gggcaggcgc ggccgtcgag aaggctgcgg
     2041 gccgggtgcg gcacgcaacg tcttcgaggg gcactccccg cgcgcgctac acaatgaggc
     2101 ggcgcgagtc ttgctcgcgc cgcctcgtgg gccatcttct cctcgcgcgg gagggttact
     2161 tgccgctctg caggcgcttg gacagcatgc cgcgcagctt gtgcacgctc tgcgtatcct
     2221 gctggcggag ctggttcagg aagctggtga cttcctggtc attggcgtcc tcggcgtcct
     2281 ccaggtagac gtcgatcgcc gagatcccct ccagcttgtt ggt
//
LOCUS       DASUBI010000494         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_981974, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000494 DASUBI010000000
VERSION     DASUBI010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981974"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFU72_09505"
     CDS             <1..784
                     /locus_tag="VFU72_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEU5423762.1"
                     /translation="EAGADEIVVVGGDGTLNEVANGLLGLGPQAPRPVLSLIPAGTGR
                     DVARSLGIANAAQAIALIGHGEPCPIDAGVIHYRDAGAARSRYFVGFADVGLGAETAA
                     RLNRSSKALGGFLSYLTGVTLTIATFHGREARVAVDGQLVHQGRIGMVVLSNGRYLAG
                     GMDMAPMASMCDGRFEVFVLRDVPKRVLLGSLLPRVYRGRHVGHWAVRHFQGQCVEVS
                     APEPVPFQSDGEQPGTTDIRAEMIAGALLMRAPRREPARAAI"
     gene            888..2033
                     /locus_tag="VFU72_09510"
     CDS             888..2033
                     /locus_tag="VFU72_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5423763.1"
                     /translation="MNLQGDYKESGIGLAPEPTDEQFTILEGATSPALSWFVEGLTRE
                     LERHGYSHSHRAVPDIRLVLNVVDRDNPQPYRRKAQATFVVAIVELDHYPDDFLRTGY
                     PYLVRALSNLGIMLTRGADGLEAHFMTMEQGHYVVRHTPGHDAEFFEHIYSRVRPLAA
                     SQLVINNVYNADLETSLHQGDEITEQLIRSGRKLDAMNLLPAPFPIHEILAPREMKHV
                     MRLYGIGGLSYGNLSARKDDKRYWMSASGVNKGKLETIGRDILLVSDFDPEQPAMILS
                     VPPEVQPRRVSVDAIEHWMIYQEHPEVGAIVHVHAWMEGIQSTEVNFPCGTIQLAQAV
                     AELVRQEPEPGRAVIGLKNHGVTITGSSLDEIFARIEGKIIPQVPMS"
     gene            2051..>2313
                     /locus_tag="VFU72_09515"
     CDS             2051..>2313
                     /locus_tag="VFU72_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423764.1"
                     /translation="MSTLANIVTRWMAPAALLGGAVWVLRAVWLALLPAGCVGSACAE
                     SGRVARESVIAAPLLALAIALMAFAVIGMMVQAFQADRMGWLGR"
BASE COUNT          369 a          792 c          776 g          376 t
ORIGIN      
        1 cgaggccggc gcggacgaga tcgtggtcgt cggcggcgac ggtacgctca acgaggtcgc
       61 caacgggctg ctcggcctcg gtccgcaggc gccgcggccg gtgctctcgc tgatcccggc
      121 cggcaccggg cgcgacgtgg cgcgctcgct gggcatcgcc aacgcggccc aggctatcgc
      181 cctgatcggc cacggtgagc cctgcccgat cgacgcgggt gtgatccact atcgcgacgc
      241 cggcgcggcg cggtcgcgct acttcgtcgg cttcgccgat gtcggcctcg gcgccgagac
      301 agccgcccgc ctcaaccgct cctccaaggc cctcggcggc ttcctctcct atctcaccgg
      361 cgtcacgctc accatcgcga cctttcacgg tcgggaggcg cgcgtggcgg tcgacggcca
      421 actcgtccac cagggacgga tcggcatggt ggtgctctcc aacgggcgct acctcgctgg
      481 cgggatggac atggcgccga tggcctcgat gtgcgacggc cgcttcgagg tcttcgtgct
      541 gcgcgatgtg cccaagcgag tgctgctcgg ctcgctcctg ccgcgcgtct accgcggcag
      601 gcacgtcggc cactgggcgg tgcgtcactt ccagggccag tgtgtcgagg tcagcgcgcc
      661 cgagccggtg ccgttccagt ccgacggtga gcagccgggc acgaccgata tccgggccga
      721 gatgatcgcg ggggcactcc tgatgcgggc gccacggcgc gagccagctc gcgccgcgat
      781 ttgaccgggc catgccatgt ctgataagaa tgccgttgtg accggtcgcg acatgacggc
      841 acgacgtttc agcctgcgcg ctggagtagt gaagcggaac aaacttgatg aatctgcagg
      901 gggactataa agagagcgga attggcctgg cgccggagcc gaccgacgag caattcacca
      961 ttctcgaagg cgcgacctcc ccggcgcttt cctggttcgt cgaggggctg acccgcgaac
     1021 tggagcgcca cggctacagc cacagccacc gcgccgtccc cgacattcgc ctggtgctca
     1081 acgtggtcga ccgtgacaac ccgcagccct accgccgcaa ggctcaggcg accttcgttg
     1141 tcgcgatcgt cgaactggac cactacccgg acgacttcct gcgcaccggt tatccctacc
     1201 tggtgcgcgc cctctccaac ctcggcatca tgctgacgcg aggcgccgac ggcctggaag
     1261 cgcacttcat gaccatggag caggggcact atgtcgtgcg ccacacgccg ggccacgacg
     1321 ccgagttctt cgagcacatc tactcgcgcg tgcgtccgct agccgcctcg cagctggtga
     1381 tcaacaacgt ctacaacgcc gatctcgaaa cttcgctgca ccagggcgac gagatcaccg
     1441 agcagctcat tcgctccggg cgcaagctcg acgcgatgaa tctgctgccc gctcccttcc
     1501 cgatccacga gatcctggcg ccgcgcgaga tgaagcacgt catgcggctc tacggcatcg
     1561 ggggactcag ctacggcaac ctcagcgcgc gcaaggacga caagcgctac tggatgagcg
     1621 ccagcggggt caacaaaggg aagctggaga cgatcggccg cgacatcttg ctggtatccg
     1681 actttgaccc ggagcagccg gccatgattc tcagcgtacc gcccgaggtt cagccgcggc
     1741 gcgtctcggt cgatgccatc gagcactgga tgatctacca ggagcacccc gaggtcggcg
     1801 ccatcgtcca cgtgcatgcc tggatggagg gcatccaatc gaccgaggtg aacttcccct
     1861 gcggcacgat tcagctcgcg caagcggtcg ccgagctggt gcgacaggag ccggagccgg
     1921 ggcgcgcggt catcgggctg aagaaccacg gggtgaccat caccggcagt agcctggacg
     1981 agatcttcgc gcgcatcgag ggcaagatca tcccgcaggt gccgatgagc tagagacgag
     2041 cccgacggca atgtcgaccc tggccaacat cgtcacacgc tggatggcgc cggctgcgct
     2101 gctcggcggc gccgtctggg tgttgcgcgc ggtctggctg gcgctgctgc cggcgggctg
     2161 tgttggttct gcctgcgccg agtcgggccg tgtcgcgcgt gagagcgtca tcgcggcacc
     2221 gcttctggcg ctggcgatcg cgctgatggc gttcgcggtc atcggcatga tggtgcaggc
     2281 gttccaggcc gatcgcatgg gctggctcgg ccg
//
LOCUS       DASUBI010000495         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_260266, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000495 DASUBI010000000
VERSION     DASUBI010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260266"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2289)
                     /locus_tag="VFU72_09520"
     CDS             complement(<1..2289)
                     /locus_tag="VFU72_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5423765.1"
                     /translation="MTAAARPPGPPHAPLAALTPTRYDRTPTLSQDERAALAFVVAER
                     AQRPHRGHWPAHIHAALARLLTPVADAVATLGIVGVQRYQTAALLQRVMHERQTAYWA
                     WTGAEWREIFQTHIRGTDCRQQALACMYLLGGVDDVRVPGVVCQLPRFAAAMLGADAV
                     TDATARIRQELHGWGWTEHGAAQVLHRAVSVLLLASRNPRLEGITLPVVQAVLAATPP
                     GTAAAHLQLVARALVGLGILRTLPESPPPEEAWPRRVERLVGVPTTWAAWCARWYDTS
                     TLGLHTRRSAYYLLLKTGRWLAATHPEAVTPTAWTRDLAAEAVAVIDRLTYGAWVQVP
                     AADRFATRHGQPLSAHAKLHHLAVLRTFFRDCQEWGWLPRRFDPGRVFAASRALRAAA
                     GPAPRVIADDVWAKLLWAGLTLGAEDLPVIPVADAPWYPLALVRALVVAWLFTGLRWD
                     ELRRLRVGCIRWQRGDVTVAGVGAPLPQDAVCWLDVPVNKTMAAFTKPVDRVVGEAIA
                     AWEALRPAQPALVDAKTGAAVDYLFLYRGRRLGPAYLNKMLIPLLCRRAGLPRRDARG
                     SLSSHRARSTIASQLANAREPLTLLELMAWLGHRSPATTQHYVAVAPTRLARAYADAD
                     YFRRNIRRVEVLIDQAAVRTGAAATGTPWRFYDLGHGYCTYDFFDQCPHRMACAKCAF
                     YRPKESIAAQLLEGRANLLRLQQEIPLTEEERAAVEDGLAAHARLLAQLTDVPTPAGP
                     TPQELRGDQCAAGQAPAGSAAPF"
     gene            complement(2286..3320)
                     /locus_tag="VFU72_09525"
     CDS             complement(2286..3320)
                     /locus_tag="VFU72_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU5423766.1"
                     /translation="MRWERYPRLAQSPQARAWLTIQANLGRAANTIDAYGRALEDYLA
                     FSLRQGVPPEQAGRDHIAAYLHDLRRRPPRPGAPVPVLGTGTGLANATLQQRLTAIRL
                     YYDYLMEEGLRAENPVGRGRYTPGTAFGGTTRGLLPRFHTLPWIPDDAAWLALLEAAR
                     AEPARNRVMFALAYDAGLRREELCALETGDLDPAARLLRVRAAVTKGHRERIVPYSAP
                     TGALYAAYLAERRHLSQARGPLFLSASRRNRAQPITIWTWSKVVAALARRAGVPQFTT
                     HTFRHLCLTDLARAGWALHEIALFAGHRHPQTTLQYIHLSGCDLAAKLERGMAEIHTW
                     RTTLTAERLA"
     gene            complement(3421..3612)
                     /locus_tag="VFU72_09530"
                     /pseudo
     CDS             complement(3421..3612)
                     /locus_tag="VFU72_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          528 a         1236 c         1376 g          472 t
ORIGIN      
        1 aaaaggtgcg gcactcccgg cgggagcctg accagcggcg cactggtcgc cgcgtaactc
       61 ctgtggtgtt ggtcctgcgg gcgtcggcac atcggtcaac tgggcgagca gccgcgcatg
      121 cgccgcgagg ccatcctcca cggcggcgcg ttcctcctct gtgagcggga tctcctgctg
      181 gaggcggagc aggttcgctc gcccttccag gagttgcgcc gctattgact ccttcgggcg
      241 gtagaacgcg catttcgcgc aggccatccg atgcgggcac tggtcgaaga agtcgtaggt
      301 gcagtagccg tgccccaagt cgtagaagcg ccagggtgtg cccgtcgcgg ccgctccagt
      361 ccgcaccgcg gcctggtcga tgaggacctc gacacgccgg atattccggc ggaagtagtc
      421 ggcatcggca taggcgcggg ccagccgcgt cggcgccacg gccacgtaat gctgcgtcgt
      481 tgcgggcgac cggtggccca gccaggccat cagttccagc aacgtcagcg gctcgcgggc
      541 gttggccagt tgcgaggcga tggtcgagcg ggcccggtgg ctgctcaacg acccccgcgc
      601 gtcgcgcctg ggcaggcccg cgcggcgaca cagcagcggg atgagcatct tgttcaggta
      661 ggccgggccc aagcggcgac cccgatagag gaagagatag tccacggccg ccccggtctt
      721 ggcgtcgacc agggctggtt gggccgggcg gagcgcttcc caggcggcga tggcctcccc
      781 gaccacgcgg tcgaccggct tggtgaaggc ggccatggtc ttgttgaccg gcacgtcgag
      841 ccagcagacc gcgtcctgcg ggagcggcgc accgacgccc gccacggtga cgtcgccgcg
      901 ctgccagcgg atgcagccga cgcgcaagcg gcgcagctcg tcccagcgca ggccggtgaa
      961 gagccaggcc acgaccagcg cgcggaccag cgcgagcgga taccagggcg cgtcggccac
     1021 cgggatgacc gggagatcct ccgcgccaag ggtgagcccc gcccacaaga gcttggccca
     1081 gacgtcgtcg gcgatgaccc gtggcgccgg gccggcggcg gcgcggaggg cgcgggaggc
     1141 ggcgaacacg cgcccggggt cgaagcgccg tgggagccag ccccactcct ggcagtcgcg
     1201 gaagaaggtg cgcagcaccg cgaggtggtg cagcttggca tgggccgaca gcggctggcc
     1261 gtggcgcgtc gcgaagcggt ctgcggccgg gacctgcacc cacgccccgt aggtcagccg
     1321 gtcgatgacc gccacggcct ccgccgccag gtcgcgtgtc caggccgtcg gcgtcaccgc
     1381 ctccgggtgg gttgccgcca gccagcgacc cgtcttgagc aggaggtagt acgccgagcg
     1441 acgggtgtgc agtcccagcg tggaggtgtc gtaccaccgc gcgcaccacg cggcccaggt
     1501 ggtgggcacc ccgaccagcc gttccacgcg tctcggccac gcctcctccg gcggcggcga
     1561 ctcgggcagg gtgcgcagga taccgagccc gaccagggcg cgggcgacca gctggaggtg
     1621 cgcggccgcc gtcccggggg gcgtggccgc cagcacggcc tgcaccacgg gcagggtaat
     1681 cccctcgagg cgcgggttgc gactcgccaa caggagcaca ctcacggccc ggtgcagcac
     1741 ctgggccgcg ccgtgctccg tccagcccca gccgtggagc tcctgtcgga tgcgtgcggt
     1801 ggcgtcggtc accgcgtccg cgccgagcat cgcggcggcg aagcggggca gctgacacac
     1861 cacgccgggc acccgcacat catcgacgcc gccgagcagg tacatgcagg cgagggcctg
     1921 ctggcgacag tcggtcccgc ggatatgcgt ctggaagatc tcccgccact ccgcgccggt
     1981 ccaggcccag taggccgtct ggcgctcgtg catgacccgc tgcaggagcg cggcggtctg
     2041 gtagcgctgg accccgacga tcccgagggt ggccacggcg tcggcgacgg gcgtgagcag
     2101 gcgcgccagc gcggcgtgga tgtgcgccgg ccagtggccg cgatggggcc gctgggcgcg
     2161 ctccgcaacg acaaacgcca gggccgcgcg ttcgtcctgg ctgagggtcg gcgtccggtc
     2221 ataccgggtg ggcgtcagcg cggcaagtgg ggcgtgcggc gggccgggtg gccgtgcagc
     2281 ggcggtcatg cgaggcgctc cgccgtcagg gtggtgcgcc aggtgtggat ctcggccatg
     2341 ccgcgctcca gcttggccgc gaggtcacag cccgagaggt ggatgtactg cagcgtcgtc
     2401 tgcgggtggc gatgcccggc gaacagcgcg atttcgtgga gcgcccaccc ggcgcgcgcg
     2461 aggtcggtga ggcacagatg ccggaaggta tgggtcgtga actgcggcac gccggcccgg
     2521 cgcgcgagcg ccgcgacgac cttcgaccag gtccagatgg taatgggctg tgcccggttc
     2581 cggcgcgagg cggagaggaa gagcggcccg cgcgcctggc tgaggtgccg ccgttcggct
     2641 aagtaggccg cgtagagcgc cccggtcggc gcggaatagg gcacgatgcg ctcgcggtgc
     2701 cccttggtga cggcggcacg cacgcgcagc agccgcgcgg cggggtcgag gtcgcccgtc
     2761 tccagcgcgc agagctcttc gcggcgcaga ccggcgtcgt aggcgagggc gaacatgacc
     2821 cgattgcgcg cgggctcggc ccgcgccgcc tccaggaggg ccagccaggc ggcgtcatcc
     2881 gggatccaag ggagggtgtg gaagcgcggc aggagcccgc gggtggtccc gccgaaggcc
     2941 gtccccggcg tgtagcggcc gcgccccacg gggttctcgg cacgcaggcc ctcttccatc
     3001 aggtagtcgt agtacaagcg gatcgccgtc agccgctgct ggagcgtcgc gttggcgagg
     3061 ccggtaccgg tgccgaggac cggcaccggc gcgccaggtc ggggtggtcg acggcggagg
     3121 tcatggagat aggcggcgat atggtcgcgc ccggcctgct cgggcggcac cccctggcgc
     3181 aggctgaacg cgagatagtc ctccagcgcg cgtccgtagg cgtcgatggt attggcggcg
     3241 cggcccaggt tggcctggat ggtgagccag gcccgggctt gaggggattg cgccagccgc
     3301 ggatagcgct cccaccgcac cgtcaacagg gcacctcctt gcccagcaag cattgcaccg
     3361 ccatcgagag tctaccacag gtgtacgttc actacccgac cacagattcc acgtaacata
     3421 taggggtagg cacagcgcgt acttccagtc gatgcgactg cgcaccgcgt aggccgcctg
     3481 gcggtccgac aaaccctcgg cgaactgcag gatggtcgcc agggccagcc gccacggtgc
     3541 cagggcgggc tgcccgtgag ccggaaacag atcggcaaag tcctcgtcgg cgaacaactc
     3601 gcccagttcg tc
//
LOCUS       DASUBI010000496         8359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_136758, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000496 DASUBI010000000
VERSION     DASUBI010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136758"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..243
                     /locus_tag="VFU72_09535"
     CDS             118..243
                     /locus_tag="VFU72_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423767.1"
                     /translation="MRCRPYRPASLVWYAVLTASVALRPLVSLLPVIEERVLVTR"
     gene            286..672
                     /locus_tag="VFU72_09540"
     CDS             286..672
                     /locus_tag="VFU72_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423768.1"
                     /translation="MSMDALLRLLGVIAFVGLPALGFWLQSQTHLLRGSNSSTGAMIG
                     FLLVGIISGALIRSWWFLGGIATLFGVYTAAILVGPLTPSPDDTPIMVVFLVAVLAFV
                     PLFAGAIAGTALGLAAERHAGRQRAH"
     gene            791..1879
                     /locus_tag="VFU72_09545"
     CDS             791..1879
                     /locus_tag="VFU72_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5423769.1"
                     /translation="MSDSAGEPQRPPRRVTTRIEVPPRTIVTIILVVAAIWLLGRLWH
                     QVLLFFIALLIAAALDPLLLRLERRGWPRGLAVALMMAAFVGIIALLLTLVVPPVIQQ
                     GERLAENLPGYAEDLQGMLKGYPTVQEWLQQNANQTAGDPKAIFGGVLSFGTGIFSGI
                     ASLLILIALTVYLLLDGPRLFAWLTGSLSPARAVKAERMRRDISHVVGAYVRGQLIVS
                     TLFGVFTYITLSLAGTPEPLLLAVLAAFLDAIPLVGATLATVPAVLLTLTVSLPAALV
                     VLAIYIVYQQTENYVIIPRVFRGALQIPSLVVLVAVIVGSGLLGIVGALLSLPVAAAI
                     PAVARAWREVMPPPEIGPEAEPEAKEPA"
     gene            2023..2979
                     /gene="lipA"
                     /locus_tag="VFU72_09550"
     CDS             2023..2979
                     /gene="lipA"
                     /locus_tag="VFU72_09550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5423770.1"
                     /translation="MEQTQGHGQRAATRQPILLQKKPEWFKVRRREGENYLELKQIMR
                     EGALHTVCEEAHCPNIYECWEQRTATFMILGDTCTRACRFCSVKWGRPSHVDRLEPRR
                     VAAAVAAMDLKFAVVTSVNRDDLHDGGAGIFADTIRAIRAQVPDCGVEVLIPDFQGNW
                     EALALVVDARPEILNHNVETVPRLFRRIQPWDSYETSLELFRQVRARDPRMIIKTGVM
                     VGLGETREELIGVMRDLRACDVDVLTIGQYLPPSHRHFPLQRYYTLEEFEDLRRAGLE
                     LGFGHVESGPLVRSSYHARDQAEALTLRRLQAQKQASAPAVD"
     gene            2987..3709
                     /gene="lipB"
                     /locus_tag="VFU72_09555"
     CDS             2987..3709
                     /gene="lipB"
                     /locus_tag="VFU72_09555"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5423771.1"
                     /translation="MTGTIAVVRAGRQPYRPTWELQRRLALARRRGLIEDVLLLVEHP
                     PTYTNGRGGSWENLLIGPDELAALGAECVTADRGGDITFHGPGQLVAYLIHDLGRGER
                     SVRRYVARLEESVIRTLAGFGVAGAAEPGYPGVWLGRDKIAALGVAVSRGVTYHGIAL
                     NVDVDLRFFDYMIPCGLPGRGATSLARALGQAPPLAAVTDRFIASVPDVFESDMTEGL
                     TLDDLRGIGERAVEGETTVGRA"
     gene            complement(3867..4427)
                     /locus_tag="VFU72_09560"
     CDS             complement(3867..4427)
                     /locus_tag="VFU72_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEU5423772.1"
                     /translation="MPGRADTSQVFQLKVTLRGSKPPIWRRLQLRGSTTLPELHEILQ
                     IVMDWDDAHLHSFTAGGVEYGVPEPGGFDAMRSEKRARLDRLVRNPKDRIRYEYDFGD
                     SWDHDLVVEKVLPADPTVRYPVVLAGRRAAPPEDVGGIWGYAHFLQSLNDPADPEHEM
                     YTEWIGGDFDPEAFDLDELNRALQRV"
     gene            complement(4455..4847)
                     /locus_tag="VFU72_09565"
     CDS             complement(4455..4847)
                     /locus_tag="VFU72_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423773.1"
                     /translation="MASAAARQPDADVIAQTARQTALNLAVDDTYGLWELLWGLHTDF
                     PQAPPPHLQSIACRVLADLIREGLIALVSGRAGGKEEPLPAAQALNALDQLASWAEPQ
                     RLGDAETRFVATEAGRHAYFGEARPVKS"
     gene            complement(5194..6057)
                     /locus_tag="VFU72_09570"
     CDS             complement(5194..6057)
                     /locus_tag="VFU72_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5423774.1"
                     /translation="MPSDKFEKFTERARKVLTLAQEEAHRFDHNYIGTEHLLLGLARE
                     GDGVAARVLANMGVDAAKVRGAVEFIIGRGDALIVGEIGLTPRAKKVIELAVDEARRL
                     NHHYIGTEHLLLGLVREGEGIAAGVLVSLGVNLEKVRQQVLQAVSPGAAPPPEESPKR
                     PQLRSLGQPRRVLALNQTQRQSGVTVTILSLEDYQQGAIVQGLIRPEGPLGPPDHPPP
                     IPRRVEASDELGTRYLWAFPNSFFDADSFHFSLRLEPALSPEAHTLRLQFDTRGMHDT
                     ASRRWDFELPL"
     gene            complement(6358..6603)
                     /locus_tag="VFU72_09575"
     CDS             complement(6358..6603)
                     /locus_tag="VFU72_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423775.1"
                     /translation="MQSPLTTASAAAMPEFVDQWRAGLQRIEAEIGLGYAVAEQVLAA
                     LMRATGCSLEAAVETVLAACQPARDEEPQAASGRHAA"
     gene            6801..7721
                     /locus_tag="VFU72_09580"
     CDS             6801..7721
                     /locus_tag="VFU72_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5423776.1"
                     /translation="MSAPESIRRILVPLDGSRLAETVLPSAFAVAARTGAIVTLFHVI
                     EHNPPESVHGEPHLETFSEAAAYLGTVAERWQHAGVPIEMHIHETKAHDVAESIAQHA
                     VELQADLIAMATHGSGGIRGLLFGTIAQQALRQTSTPLLLLEPGEEEPRFVDGTILVP
                     VGGSDGEHDPALPLAVLLAQALRARLHLVQVVPTVGTVGQGQRGAATFAPSATAAVLD
                     LEGQQATQALDALRRQIDAGLTVTGEVRRGAVVEELEQAIAASGAGLVVMSTHGRGTG
                     LDGIFTGSVGARLVSRTEVPYVLLPLPRGA"
     gene            7800..>8359
                     /locus_tag="VFU72_09585"
     CDS             7800..>8359
                     /locus_tag="VFU72_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5423777.1"
                     /translation="MTTESADHARLETEAAQVAAAKHDPTAFAPLYEAYVDAIYRYAY
                     RRVGSRPEAEDVTAQTFQQALAALPNFEWRGVPFGAWLYRIAANIIARSGRGTAREVA
                     VEDVTIYAEAEGGGGDAAEDPAARVEQQAASGELLAAIRRLPLDQQRALVMRFSWGLS
                     AREIGEQLGRSEGAAKQLVLRALRALR"
BASE COUNT         1196 a         2927 c         2921 g         1315 t
ORIGIN      
        1 agtgggcttc gtctggttag cgcggttggc tttagccccg cgcgccatgg ggcacaaacg
       61 cttctcggat ggccacaatc ccgctccacg gcccacgcat ccatcggcca gccgcaggtg
      121 cggtgccgcc cgtaccgccc ggcatcgctc gtctggtacg ctgtcctcac cgccagcgtc
      181 gcgctgcgcc ccctggtctc gctcttgccg gtgatcgagg agcgcgtgct ggtgacgcgt
      241 taggcgatcc gcctgccgct ccggcgcgac cgtgagagga acggtatgtc tatggacgcg
      301 cttctacgcc tgctcggcgt cattgccttc gtcggcctgc cggcgctcgg cttctggctg
      361 cagtcgcaga cccacctgct gcgggggagc aactcgagca ccggcgccat gatcggcttc
      421 ctgctggtcg ggatcatcag cggcgcgctg atccgctcct ggtggttctt gggcggtatc
      481 gccaccctgt tcggagtcta caccgccgcc atcttggtcg gacccctgac accatcgccg
      541 gacgacaccc cgatcatggt cgtgttcctg gtcgccgtgc tcgccttcgt acccctcttc
      601 gccggggcca tcgcgggcac cgcgctcggc ctcgccgccg agcggcacgc cgggcgccag
      661 cgcgcacact aggtaccgtg tgatgaaccc aacaggttcg tcaggcagcc cctccctgga
      721 caggccgcgc gcgtccgtat aggctccgac cgtgcgcgac ggcctggagc ggcgcgggga
      781 gggggagtgc gtgagcgact cggctggtga gccgcagcgg ccgccccggc gggtcacgac
      841 ccgcatcgaa gtcccgccgc gcaccatcgt caccatcatt ctagtggtcg cggcgatctg
      901 gctgctcggg cgcctctggc accaggtgct cctcttcttc atcgccctcc tgatcgcggc
      961 ggcgctcgac ccgctgctgc tgcggctgga gcggcgcggc tggccgcgcg ggctggcggt
     1021 ggcgctcatg atggccgcct tcgttggcat catcgcgctg ctgctgacgc tggtggtgcc
     1081 gccggtgatc cagcagggcg agcggctggc cgagaacctg ccgggctacg ccgaggacct
     1141 ccaggggatg ctgaagggct acccgaccgt gcaggagtgg ctgcagcaga acgccaacca
     1201 gacggccggc gaccccaaag cgatcttcgg cggggtgctc tccttcggca ccggcatctt
     1261 ctccggtatc gccagcctcc tgatcctgat cgcgctgacc gtctacctgc tgctcgacgg
     1321 tccgcggctc ttcgcctggt tgactggcag cctctcacca gcgcgcgccg tcaaggcgga
     1381 gcgcatgcgg cgagacatca gccacgtcgt cggggcctat gtgcgcgggc agttgatcgt
     1441 ctccaccctc ttcggcgtct tcacctacat caccctctcg ctggccggca cgcccgagcc
     1501 gctgctactg gcggtgctgg ccgccttcct cgacgccatc ccgctggtcg gcgccacgct
     1561 ggcgaccgtg ccggccgtgc tgctgaccct gaccgtctcg ctgccggcgg cgctggtcgt
     1621 gctggccatc tacatcgtct accagcagac cgagaactac gtcatcatcc cgcgcgtctt
     1681 ccgcggcgcg ctccagatcc cctcgctggt cgtattggtg gcggtgatcg tcggcagcgg
     1741 tttgctcggt atcgtcggcg cactcctctc gctgccggtc gcggcggcca tccccgcggt
     1801 ggcgcgtgcc tggcgcgaag tgatgccgcc gcccgaaatc gggccagagg ccgagccgga
     1861 ggccaaggaa ccggcctgag aggcgagcgg ccaacccgga gcaccgggaa tacttgccgg
     1921 gcgtgatatc gcttaggcta gcagcaggcc gtcgccggct cgtggcgcag agggaggcca
     1981 cgcgcggccg ccgggcgcga cggccggatg agggaaagcc caatggaaca gactcaggga
     2041 catggccagc gagccgccac gcggcagccg atcctcctgc agaagaagcc ggagtggttc
     2101 aaggtccggc ggcgcgaggg tgagaactac ctcgaactga agcagatcat gcgcgagggc
     2161 gcgctgcata ccgtctgcga ggaggcccac tgccccaaca tctacgagtg ctgggagcag
     2221 cgcaccgcca ccttcatgat cctgggcgac acctgcaccc gcgcctgccg cttctgcagc
     2281 gtcaaatggg gccgccccag ccacgtcgac cggctggagc cacgccgggt ggccgccgcc
     2341 gtggccgcca tggacctcaa gttcgccgtg gtcacctcgg tcaaccgcga cgacctgcac
     2401 gacggcggcg ccggcatctt cgccgacacc atccgcgcca tccgcgccca ggtgccggac
     2461 tgcggcgtcg aggtgctgat ccccgacttc cagggcaact gggaggcgct ggcgctggtg
     2521 gtcgacgccc ggcccgagat cctcaaccac aacgtcgaga ccgtgccgcg cctcttccgc
     2581 cgcatccaac cctgggactc ctacgagacc tcgctggagc tcttccgcca ggtgcgcgcc
     2641 cgcgaccccc ggatgatcat caagacgggc gtgatggtcg gcctgggcga gacgcgcgag
     2701 gagctgatcg gggtgatgcg cgacctgcgc gcctgtgatg tcgacgtgct gacgatcggg
     2761 caatacctgc cgccctcgca ccgccacttc ccgctgcagc gctactacac actggaggag
     2821 ttcgaggacc tgcgccgggc ggggctggag ctgggcttcg gacacgtcga gtccggcccg
     2881 ctggtgcgct cctcctacca cgcgcgcgac caggccgagg cgctgacgct gcggcggctg
     2941 caagcccaaa agcaggcgag cgccccggcg gtcgactgag gcgggcgtga ctgggacgat
     3001 cgcggtagtg cgggcgggcc ggcagcccta tcgcccgacc tgggagctgc agcggcggct
     3061 ggcgctcgcc cggcgccggg ggctgatcga ggacgtgctg ctcctggtcg agcacccgcc
     3121 gacctacacc aatggccgcg gcggctcctg ggagaacctg ctgatcggcc cggacgagct
     3181 ggcggcgctg ggtgccgagt gcgtcaccgc cgatcgtggc ggcgacatca ccttccacgg
     3241 ccccggccag ctcgtcgcct acctgatcca cgacctcggt cgcggcgagc gctcggtgcg
     3301 ccgctacgtg gcgcggctgg aagaaagcgt gatccgcacc ctggcgggct tcggcgtggc
     3361 gggcgcggcc gagccgggct atcccggcgt ctggctcggg cgcgacaaga tcgcggcgct
     3421 gggggtggcg gtcagccgcg gcgtgaccta ccacggcatc gcgctcaacg tcgatgtgga
     3481 tctgcgcttc ttcgactaca tgatcccctg cgggttgccc ggccggggcg ccacctcgct
     3541 ggcgcgcgcg ctgggccagg cgccgccgct cgccgccgtg accgatcgct tcatcgccag
     3601 tgtgcccgac gtcttcgagt ccgacatgac ggaagggctg acgctggacg acctgcgtgg
     3661 gattggggag cgagcggtgg agggggagac aactgtcggg cgcgcttgac tttcactcgg
     3721 acgtctgtgc ccgggggagc gagggcagac atggaggtct gtccctaccg taattgcgac
     3781 attcctattt cctgtagggg cggacctctg tgtccgccct cgtccctcta cgcctcgcca
     3841 tcccggtagg ccacgagact gccccctcac acccgctgca gtgcgcggtt gagctcgtcg
     3901 aggtcgaagg cttcggggtc gaagtccccg ccgatccact cggtgtacat ctcgtgctcg
     3961 gggtcggcag ggtcgttgag gctttgcaag aaatgggcgt agccccagat gccgccgaca
     4021 tcctccggcg gggcggcgcg gcggccggca agcaccaccg ggtagcgcac cgtggggtcg
     4081 gcgggcagca ccttctcgac caccagatcg tggtcccagc tgtcgccgaa gtcgtactcg
     4141 tagcggatac ggtccttggg gttgcggaca aggcggtcga gccgcgcccg cttctcgctc
     4201 cgcatcgcgt cgaacccgcc cggttcgggc acgccatact ccacgccccc cgcggtgaag
     4261 gagtgcaggt gcgcgtcatc ccagtccatg acgatctgga ggatctcgtg cagctcgggc
     4321 agcgtggtgc tgccgcggag ctgtaggcgg cgccagatcg gcggcttgct ccccctgagg
     4381 gtcaccttga gctgaaagac ctggctggta tcggctctcc cgggcatcgt gctcctcccc
     4441 tgggacaggc cgcgctatga ctttacgggc ctggcttccc cgaaatacgc atgacgcccg
     4501 gcctccgtcg ccacgaaccg cgtttcggca tcaccgaggc gttgtggttc ggcccaactc
     4561 gcaagttggt cgagcgcgtt cagtgcctgc gcggccggca gtggttcctc cttgccaccg
     4621 gcacggccgg aaacgagggc gatcagcccc tcacggatga gatccgcgag cacccggcac
     4681 gcgatcgact gaagatgcgg gggcggcgcc tgggggaagt cggtgtgcaa cccccagagc
     4741 agctcccaca aaccgtaggt gtcgtccaca gccaggttga gtgccgtctg ccgcgcggtt
     4801 tgcgcgatta catcggcatc gggttgcctt gcagcagcac tcgccatcga ttgccgtccc
     4861 tactgatgac cgcatagggc ccctgtttct ccgggtctgg atcaggggga ttcccccaca
     4921 tgatccgaat ctgatttccc gggcggtttg ggtcgctata gcggaaacca gcgctatttc
     4981 gtgccgggcg ccggggccaa tctcgcggga tgagcgcttc aatctcctgg atgctcgcgc
     5041 cgtggagcga atcgatgtcc gcgagcgagc gatacgacgc caaactccac cccgcgaggt
     5101 catcctgcca gttgcatgcc tccgcttata gcgggatgcg gcttcactgt caagctacgg
     5161 cggatcctgc gtggaccgat caccacgccg ccctcacagc ggcagttcga agtcccagcg
     5221 gcgcgaggcg gtgtcgtgca tgccgcgcgt gtcgaattgc aggcgcagcg tgtgggcttc
     5281 cgggctgagc gcgggctcca ggcgcaggct gaagtgaaac gagtcggcgt cgaagaagct
     5341 attcgggaac gcccacagat agcgcgtgcc cagctcgtcg ctcgcctcga cgcgccgcgg
     5401 tatcggaggt gggtggtccg gcggtccaag cggaccttcc gggcggatca ggccctggac
     5461 gatcgcgccc tgctggtaat cctcaagcga gaggatggtg acggtcacgc cggactgacg
     5521 ctgcgtctgg ttgagggcca gcacacggcg cggctggccg agcgagcgca gttgcgggcg
     5581 cttcggggat tcctcgggcg gcggcgcggc gccggggctg accgcctgca gcacctgctg
     5641 gcgcaccttc tccagattca cacccaggct caccagcaca cccgccgcga taccctcgcc
     5701 ctcacgaacg aggccgagca acaggtgctc ggtgccgatg tagtggtggt tgagacgccg
     5761 cgcctcgtcc accgccagct cgatgacctt cttggcgcgc ggcgtcagcc cgatctcgcc
     5821 cacgatcagc gcgtcgcccc ggccgatgat gaactcgacc gcgccgcgca ccttggcggc
     5881 atcgaccccc atgttggcca gcacgcgggc ggcgacgccg tcgccctcgc gcgccagacc
     5941 cagcagcagg tgctcagtcc cgatgtagtt gtggtcgaag cggtgcgcct cttcctgcgc
     6001 cagcgtcagc accttgcgcg cccgctcggt gaacttctcg aatttgtctg atggcatcgc
     6061 gcgcctcctc ccggcggggc cgcgtgacat ggaggatcga tcccatgata gcccattggg
     6121 agcgggaacg tactgactgt ggaagccggc gacgggttgg tggaggcggt ggcagtcgtg
     6181 gagggtgggt gccgggtgcg gacggatccc tccactgcgg tcgggatgac agggagagta
     6241 aggggcggac gaggaggatc agtgccgtcc gataggcccc gacattaacc ctgtcatccc
     6301 gaccgcagtg gagggatccg tcgtggagtg gcacgcgacg tgttcgtcag gcagcggcta
     6361 ggcggcgtgg cggccgctgg cggcctgggg ctcctcgtcg cgcgccggct ggcaggccgc
     6421 cagcacggtc tcgaccgccg cctccagcga gcagccggtg gcgcgcatca gcgccgccag
     6481 cacctgctcg gccacggcgt agcccagccc gatctcggcc tcgatgcgct gcaagcccgc
     6541 gcgccattgg tcgacgaact ccggcatcgc ggccgcggaa gcagtggtaa gcggtgactg
     6601 catggcgata cctcccgatg gccgtagtat acgagtgtac gtacaccgcg tcaaggggag
     6661 ttgccgctgg cgcagccgga cgcgcacggg ttgcgcgcct ccggcattcc cgccacgcgc
     6721 gccgcgctgg tacgcttggg cctgcgacgg caatatgtcg ccgctcgccg gccatgcgac
     6781 ccgaccgaag gaggtgagct atgagcgcgc ccgaatcgat ccgccgcatc ctcgtaccgc
     6841 tggacggctc acggctggcc gagaccgtct tgccctcggc cttcgccgtg gcggcgcgca
     6901 ccggcgcgat cgtcacgctc ttccacgtga tcgagcacaa cccgcccgag agcgtccacg
     6961 gcgagccgca cctggagacc ttctccgagg ccgccgccta cctcggcacc gtggccgagc
     7021 gctggcagca tgccggggtg ccgatcgaga tgcacatcca cgagaccaag gcgcacgacg
     7081 tggccgagag catcgcgcag cacgccgtcg agctgcaggc cgacctgatc gccatggcga
     7141 cccacggctc gggcggcatc cgtggcctcc tcttcggcac catcgcccag caggcgctgc
     7201 gccagaccag caccccgctg ctgctgctgg agcccggcga ggaggagccg cgcttcgtgg
     7261 acggcaccat cctggtgccg gtcggcggca gcgacggcga gcacgacccc gccctgccac
     7321 tggccgtgct gctggcgcag gcgctgcgcg cccggctgca cctggtccag gtggtgccga
     7381 cggtcggcac ggtcggccag ggacagcgcg gcgcggcgac cttcgcgccc tcggccacgg
     7441 ctgcggtgct cgacctggag gggcagcagg cgacgcaggc gctggacgcg ctgcggcggc
     7501 agatcgacgc cgggctgacc gtcaccggcg aggtgcggcg cggcgccgtg gtcgaagagc
     7561 tggagcaggc gatcgccgcg agcggcgccg ggctagtggt gatgagcacg cacgggcgcg
     7621 gtaccgggct ggacggcatc ttcactggga gcgtcggcgc ccgcctggta agccgcaccg
     7681 aggtgcccta tgtcctgctg ccgcttccac gcggcgcgta accttctcgg cgcggaggca
     7741 gtgttgagag tggcgggcgg tgagtggacc ggggacgccg ggcgggccac aataggagca
     7801 tgacgacgga gtccgcggac cacgcccgcc ttgagaccga ggccgcccag gtggcggccg
     7861 ccaaacatga tccgacggcc ttcgcgccgc tctacgaggc ctacgtcgat gccatctacc
     7921 gctatgccta ccgccgcgtt ggctcgcgcc ccgaggccga ggacgtgacg gcgcagacct
     7981 tccaacaggc gctggcggcg ctgcccaact ttgaatggcg cggcgtcccc ttcggcgcct
     8041 ggctctaccg catcgccgcc aacatcatcg cgcgcagcgg gcggggaacc gcgcgcgagg
     8101 tcgcggtgga ggatgtgacc atctacgcgg aggctgaagg gggaggggga gatgccgccg
     8161 aggatccagc ggcgcgcgtc gagcagcagg ccgccagcgg cgagctgctg gcggcgatcc
     8221 gccggctgcc gctcgatcaa cagcgggcgc tggtgatgcg cttctcctgg ggcctgagcg
     8281 cgcgcgagat cggggaacag ttgggccgca gcgagggcgc cgccaagcag ttggtccttc
     8341 gcgcgctgcg cgcgctgcg
//
LOCUS       DASUBI010000497         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_356130, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000497 DASUBI010000000
VERSION     DASUBI010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356130"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFU72_09590"
     CDS             complement(<1..503)
                     /locus_tag="VFU72_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5423778.1"
                     /translation="MQRQSRANQSGTIALAERMGRLGTESAFDVLSRARALEAQGVDV
                     IHLEIGEPDFDTPPHIVEAAVQALRQGQTHYTPSAGLPVLREAIADYVSRTRGVVSGA
                     DRVVVTLGGKPVMFYTILALAGPGDEVIYPDPGFPIYESVINFVGAKAVPLRLREEQD
                     FGFDPEEL"
     gene            663..1205
                     /locus_tag="VFU72_09595"
     CDS             663..1205
                     /locus_tag="VFU72_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423779.1"
                     /translation="MAEFKRVRRETEVETPDVEATQPLQYQEHRVDERRVESGDDPEY
                     RREVETVRSDRAGSPRRVVENEYVERRDPYLERQIMYSKIANVVWTIVGLIEALIAIR
                     VILKLIGANAGNGFVDFIYRLSGVFVAPFLGIVNDPTSGSSILEINSLIAMLVYLILG
                     WAILKLIVLAFNVTEPPTAP"
     gene            complement(1406..>3343)
                     /locus_tag="VFU72_09600"
     CDS             complement(1406..>3343)
                     /locus_tag="VFU72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5423780.1"
                     /translation="VAAEGPPRPEQAPPRASGWIVGLAAGWGQQALLASGDAQVKPLD
                     VGQTEALRLSFTGQTTAADAVARLLQNPAVAYVEPDTLLQWYWTPNDPYYPEQGWAQV
                     DHLPDAWAVATGQASTIIAVIDTGVRPDHPDLKGKLVPGYDFFDGNDNPLDDVGHGTG
                     VAGIAAARGNDGVGIAGAAMNVQIMPLRVGNGDGAPISDVAQAIHFAVDHGAAVINLS
                     LGEVQQSATLLDAIQYAYDRNVVIVAAAGNDPNQASFPASYPQVISVGATTADGKAVA
                     SFTSQISRVDIGAPGMGVLTDWWDAASQQNGWAWVEGTSFATPMVSGTVALLKSINPK
                     LTVEQVRAILTGTAHPLSKNQGVGAGLLDAGEAVRHALLPDYVSVWQPADRPVAAGAA
                     TRTWEWGPNSWQVGTEPYTDSQQGQRLVAYFDKARMEINNPYGDPNSPWFVTTGLLVT
                     EMISGQMQVGNDSFQPRGPAQIPVAGDPDDTSGPTYASFTALTQAQPLTEGSTVLETI
                     DRSGQVGSEQRLADYAVVATDYVPDTNHRVASVFRDYLNSSGVLYQQGQFLSGQLFSP
                     AFYATGFPITEPYWARVKVGGTIEDVLVQCFQRRCLTYTPSNPTGWQVEMGNVGRHYY
                     TWRYGTQPPASSPDDPTALAR"
BASE COUNT          513 a         1162 c         1121 g          547 t
ORIGIN      
        1 agttcctcgg ggtcgaagcc gaagtcctgc tcctcgcgca gccgcagcgg caccgctttg
       61 gcgcccacga agttgatcac cgactcgtag atcgggaagc cggggtcggg gtagatcacc
      121 tcgtcgcccg gtccggccag cgccaggatg gtgtagaaca tcaccggctt gccgcccagc
      181 gtcaccacaa cgcggtcggc cccgctcacc acaccgcgcg tgcggctgac gtagtccgcg
      241 atcgcctcgc gcagcaccgg cagcccggcc gagggcgtgt agtgagtctg tccctggcgc
      301 aacgcctgga ccgcggcctc gacgatgtgc ggcggcgtgt cgaagtccgg ctcgccgatc
      361 tccaggtgaa tgacgtcgac cccctgggcc tccagcgccc gcgcccgcga gagcacgtcg
      421 aaggcgctct cggtgcctag ccggcccatg cgctccgcca aggcgatcgt gcctgactga
      481 ttcgcccgac tctgccgctg catcgcgccc ccttagcacc ctgattcaac gaccaaaacg
      541 cctaaagcgc acttcccacg ccactgtctg cgctcgcggc aggagtcttg catttagcag
      601 cgccgagtgt cgatcgctca gcagttcgag cgtcgaacga cgcgaacaag gggagatgga
      661 atatggccga attcaaacgg gtccggcgcg agacggaggt cgagactccc gacgtcgagg
      721 cgacccagcc gctgcagtac caggagcacc gggtcgacga gcgccgcgtg gagagcggcg
      781 acgatcccga ataccggcgc gaggtcgaaa cggtgcgctc ggaccgcgcc ggctcgccac
      841 gccgcgtggt cgagaacgag tacgtcgagc ggcgcgatcc ctatctggaa cgtcagatca
      901 tgtattcgaa gatcgccaac gtggtctgga cgatcgtcgg cttgatcgag gcactcatcg
      961 cgatccgggt catcctcaag ctgatcggcg ccaacgccgg caatggcttc gtggacttca
     1021 tctaccgcct gagcggcgtc ttcgtggcgc ccttcctggg gatcgtcaac gaccccacct
     1081 ccggcagctc gatcctggag atcaactcgc tgatcgcgat gctggtctac ctgatcctgg
     1141 gctgggcgat cctcaagctg atcgtgctgg ccttcaacgt caccgagccg ccgaccgcgc
     1201 cgtagtcgaa cggctgccca tcgaagacgt tgggtgccgc gtcagcacgg atccctccac
     1261 ttcggtcggg atgacaggat gaatacgggg cggacgacaa cgacacgcgt tatcgtccgc
     1321 cccaatcccc cagtcacgtc atcctgagct tgtcgaagga tccgtcctca cacgccccag
     1381 aacccctgag cagcccgaac cgccactacc gcgccagcgc cgtcgggtcg tcgggcgagg
     1441 aagcgggcgg ctgcgtgccg tagcgccagg tgtagtagtg gcggccgacg ttgcccatct
     1501 cgacctgcca gccggtgggg ttgctgggcg tgtaggtcag gcagcggcgc tggaagcact
     1561 gcaccagcac gtcttcgatc gtgccgccca ccttgactcg cgcccagtag ggctcggtga
     1621 tcgggaaccc ggtagcgtag aaggccggac tgaagagctg gccactcagg aactggccct
     1681 gctggtagag tacgccactg ctgttcagat agtcgcggaa gacgctggcg acgcggtggt
     1741 tggtgtccgg cacgtagtcg gtggcgacga cagcgtagtc agccaggcgc tgttcgctcc
     1801 ccacctggcc ggagcggtcg atcgtctcca gcaccgtgct gccctcggtg agcggctgcg
     1861 cctgggtcag cgcggtaaag ctggcgtagg tcgggccgct cgtgtcgtcg gggtcgccgg
     1921 cgaccgggat ctgcgccggg ccgcgcggct ggaagctatc gttccccacc tgcatctggc
     1981 cgctgatcat ctcggtgacc agcagaccgg tggtgacgaa ccagggactg ttgggatcgc
     2041 cgtaggggtt attgatctcc atgcgcgcct tgtcgaagta ggcgaccagc cgctgcccct
     2101 gctggctgtc ggtgtacggc tcggtgccga cctgccagga gttcggcccc cattcccagg
     2161 tgcgcgtcgc cgccccggcc gccaccggac ggtcggctgg ctgccagacg gagacgtaat
     2221 ccggcagcag cgcgtggcgc accgcctcgc cggcgtcgag caaccccgcg cccaccccct
     2281 ggttcttgct caatgggtgc gccgtgccgg tcaggatggc gcgcacctgc tcgacggtca
     2341 gtttggggtt gatcgatttc aagagcgcca ccgtgccgga gaccatcggc gtggcgaacg
     2401 aggtgccttc gacccaggcc cagccgttct gctgcgaggc cgcgtcccac cagtcagtca
     2461 gcacgcccat ccccggtgcc ccgatatcga cgcggctgat ctgtgaggta aagctggcca
     2521 ccgccttgcc gtcggcggtg gtggcgccga ccgagatcac ctgaggatag ctggccggga
     2581 acgaggcctg gttcgggtcg ttgccggccg cggccacgat caccacgttg cggtcgtagg
     2641 cgtactgaat agcgtccagc agcgtcgcac tctgctgcac ctcgcccagg ctaagattga
     2701 tcaccgcggc gccgtggtcg acggcgaagt ggatcgcctg cgccacgtcg ctgatcggcg
     2761 cgccgtcgcc gttgccgacg cgcagcggca tgatctgcac gttcatggcc gcgccggcga
     2821 tcccgacccc gtcgttgccg cgcgccgccg cgatcccggc cacgccggtg ccgtggccca
     2881 cgtcatcgag cgggttatcg ttgccgtcga agaagtcgta gcccggcacg agcttgccct
     2941 tgaggtcggg gtggtcgggc cgcacgccgg tgtcgatcac cgcgatgatg gtggacgcct
     3001 gcccggtcgc gaccgcccag gcgtcgggca ggtgatcgac ctgcgcccag ccctgctccg
     3061 ggtagtaggg atcgttgggc gtccagtacc actgcagcag cgtgtccggc tcgacgtagg
     3121 ccacggccgg gttctgcagc agccgcgcga cggcgtcggc cgccgtcgtc tgccccgtga
     3181 agctcaggcg cagggcctcg gtctggccca cgtcgagcgg cttgacctgg gcatctccgc
     3241 tggccagcaa tgcctgctgc ccccagccgg cggccagccc gacgatccag ccgctggcgc
     3301 gcggcggcgc ctgctcaggc cgcggcggac cttccgccgc cac
//
LOCUS       DASUBI010000498         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_530115, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000498 DASUBI010000000
VERSION     DASUBI010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530115"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5262
                     /locus_tag="VFU72_09605"
     CDS             <1..>5262
                     /locus_tag="VFU72_09605"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEU5423781.1"
                     /translation="IPGGTVTIAASAILANQAGGNGGGILLTGKGVATLTNTTIGANS
                     AGAHGGGLASTAGGSKSTAKSAVFTNVTVTDNTAQRGGGIDSAGTTSLFNSIVAGNHA
                     PSAADCYAGGKSPITSLGYNLIQSTASCALSGTLTGNVTGTGAQLGPLGNYGGATPSY
                     RPLLDSPVVDAGSNTNCPATDQRGVKRPGAGTGQSSCDIGAVELSTGLQISQDDNTSW
                     PHASLITPGQAVSDDALDAPGQSRWYKFHVNPSQQVEVTLTHLPANYDLTLFKDINAA
                     YLALTSVSSTTDLARLGAEFAPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSPD
                     AYTPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSPDAFSSAQTRSIIGA
                     SAFNGTAGEGIRVNTWDLSGDFYVRVRGRNGADVPGVTFTLQANVKAVGCDTVNWAEV
                     DSLLAPAPSFNTPGAQTIILTDLARMGQADPNGNSAALISALNTFAGLPSVNGVVVDV
                     DQYVQAANRLADATPTCPYLKNVVANQIKHVVDAYRQESPGLKYVVIVGGDNVIPFYR
                     YPDQALLGSEKNFYPPVKDVSASQASLREGYVLSQDRYGAALNLSAGNTTLPLPRLAV
                     GRLVETMAEVDTMLGAYGRTSGGVITPASALVTGYDFLADDAHAVLAELAAGLGAGGK
                     LDSLITNDGVDPTTPRSGAWDPTQPWTADDLKPLLLNQKHDLVFLAGHFSAGSALAAD
                     FSTRVLTSDLTTAQADLTNEIIFSAGCHSGYNTVNDDATIVTPTPDWAEAAALKGITL
                     IAGTGYQYGDTDFIKYSEQLYLNFAQQLHSGSGAVALGQALVNAKLMYLTQSPPSALS
                     GTAQKAFLEATLFGLPMLSVNLPGTRLQLQGSLKALANSAPQTFGTNPGATLGLSYQD
                     VSLDPALTPHQVTLTSPDGSQTTVTYYSGPDGVVVSPLAPVLPFVSQSADGPSGTIVR
                     GVGFFGGTYTDLSGVSPLTSAVVTEQSSTHLPFHSSYLYPAQPWALNYLGLLGGFGPP
                     LLQVTPAQYQSDSATSTTGTLRLLNDMNFRLFFSGNTATYPAGGTYPTSGDIPALAAP
                     PAISQIAATPAADGSITFAAHVAGDPLAGIQQVWVTYTVIPAGGGTGRWISLDLTQST
                     ADSTLWQGSLSPATLRQQLGTSDLTNFRFMAQAANGVGLVTLATNFGTYYQVGSGTPP
                     NPAATTLAFNGAPTGGAYGTNATFSATLSSADAACVAGQTLFFGIGAQRVPATTDAGG
                     HASATIPLLASPGDADARVTYGGNDNCNLASAETPFTITKQPSHLTLVTSDGAFPVTV
                     TLKDDNNNPLRQRSVYVAFTGAEFNDGELVTTDQAGTATIDSFDLPAGSFTVTASFGQ
                     PVTLPDNTSLDLTDQLYAGSSATGALGGGSTPASQTFLAWGRNNEGELGNGTTADSAL
                     PAPVDTSNLGDVSAFAAGDYYTIALKSDGTVWAWGSNGNHYLGDGTTAQRTTPVQVQN
                     LSSIIAISARRNHSLALKSDGTVWAWGENGSGQLGDGTTTSPPTPVQVQGLPGNITAI
                     AAGGFFSLALTSDGHIYAWGANDFGQLGNGVGGDTSAHPAPALVMMPGVVGAFTQITA
                     GLANAFAVDQNGNLWAWGDNRVGLLGIGSADSGAHATPARTFGLSHVTAIAAYYNHTL
                     AVSGGTVYAWGNGSPGVLGDGTTNNHDAPNPVPGLSNVVSVAVGGYHSLALTADGQMY
                     AWGSNSEDGRLGDNETGTGSDALSPVPV"
BASE COUNT          875 a         2067 c         1635 g          685 t
ORIGIN      
        1 atccccggcg gaacggtgac catcgcggcc agcgccatcc tcgccaacca ggccggcggc
       61 aacggcggcg gtatcttgct gaccggcaag ggtgtggcga cgctcaccaa caccaccatc
      121 ggcgccaata gcgcgggcgc gcatggcggc ggtctcgcca gcaccgccgg cggctccaag
      181 agcaccgcca agagcgccgt tttcaccaat gtcacggtga cggacaacac cgcgcagcgc
      241 ggcggcggca tcgacagcgc cggcaccacc agtctcttca acagcatcgt ggccggcaac
      301 cacgccccca gcgccgccga ctgctacgcc ggcggcaaga gcccgatcac ctcgctgggc
      361 tacaacctga tccagtcgac cgcctcctgc gcgctcagcg gcacgctgac cggcaacgtc
      421 accggcacgg gcgcgcagct cggcccgctg ggcaactacg gcggcgcgac gccgtcgtac
      481 cgcccgctgc tggacagccc ggtcgtcgac gccggcagca acaccaactg cccggccacc
      541 gaccagcgcg gcgtcaagcg acccggcgcc ggcacgggcc agtccagctg cgacatcggc
      601 gcggtggagc tgagcaccgg gctgcagatc agccaagacg acaacacctc ctggccccac
      661 gccagcctga tcacccccgg ccaggcggtc agcgacgacg cgctcgacgc gccaggccag
      721 tcccgctggt acaagttcca cgtcaacccc agccagcagg tggaggtgac gctgacccat
      781 ctcccggcga actacgatct gacgctcttc aaggacatca acgcggccta cctggccttg
      841 acctcggtct cgagcaccac cgatctggcg cgactcggcg ccgagttcgc gccggacgcc
      901 ttctcgcccg acgcgttcag tcccgacgcc ttctcgccgg acgccttcag ccccgacgcg
      961 ttctcgcccg acgcgttctc gcccgatgcg ttcagccccg acgcatacac acccgatgcc
     1021 ttctcaccgg atgcctttag ccccgatgcc ttcagccccg atgccttctc gccggatgcg
     1081 ttctcacccg acgccttcag cccggatgcc ttctcgcccg acgcgttctc gccggacgcc
     1141 ttctccagcg cgcagacgcg cagcatcatc ggcgcctcgg ccttcaacgg caccgccggc
     1201 gagggcatcc gggtcaacac ctgggatctg agcggcgact tctacgtccg cgtgcgcggg
     1261 cgcaacggcg ccgacgtccc cggtgtgacc ttcaccctac aggcgaacgt gaaggcagtc
     1321 ggctgcgaca cggtcaactg ggccgaggtc gacagcctgc tggccccggc gccgagcttc
     1381 aacacgccgg gcgcgcagac gatcatcctg accgacctgg cgcgcatggg ccaggcggac
     1441 ccgaatggca acagcgccgc cttgataagc gcgctgaaca ccttcgccgg tctgccctcg
     1501 gtcaacggcg tggtggtgga cgtcgatcag tacgtgcagg ccgccaacag gctggccgac
     1561 gccaccccga cctgccccta tctcaagaac gtggtcgcca accagatcaa gcacgtcgtc
     1621 gacgcctatc gccaggagag cccgggcctc aagtacgtcg tcatcgtcgg cggcgacaac
     1681 gtcatcccct tctaccgcta cccggatcag gcgctgctgg ggagcgagaa gaacttctac
     1741 ccgccggtca aggacgtcag cgcctcgcag gccagcctgc gcgagggcta cgtgctcagc
     1801 caggatcgct acggcgccgc gctcaacctc tcggccggca acaccaccct gccgctgccg
     1861 cggttggccg tcggccggct ggttgagacg atggccgagg tggacaccat gctcggcgcc
     1921 tacggccgga ccagcggcgg cgtgatcacg ccggcgagcg ccctggtcac cggctacgac
     1981 ttcctggccg acgacgccca cgcggtgctg gctgagcttg ccgccgggct gggcgcgggc
     2041 ggcaagctcg actcgctgat caccaacgat ggcgtcgatc cgacgacgcc ccgcagcggc
     2101 gcctgggatc cgacccagcc ctggaccgcc gacgacctga agccgctgct gctcaaccag
     2161 aagcacgacc tggtcttcct ggcggggcac ttcagcgccg gcagcgcgct ggccgccgac
     2221 ttcagcacgc gcgtcttgac cagtgacctg accacggcgc aggccgacct gaccaacgag
     2281 atcatcttca gcgccggctg ccactccggc tacaacacgg tcaacgacga cgccaccatc
     2341 gtcacgccca cgccggactg ggccgaggcc gcggcgctga agggcatcac cctgatcgcc
     2401 ggcaccggct accagtacgg cgacaccgac ttcatcaagt actccgagca gctctacctc
     2461 aacttcgccc agcaactgca ctccggcagc ggcgcggtgg cgctcggcca ggcgctggtc
     2521 aacgccaagc tgatgtacct gacgcagtcg ccgcccagcg cgctcagcgg cacggcccag
     2581 aaggccttcc tggaggcgac gctcttcggc ctgccgatgc tcagcgtcaa cctgccgggc
     2641 acccgcctgc aattgcaggg ctccctcaag gcgctggcca acagcgcgcc gcagacgttc
     2701 ggcaccaacc ccggcgccac gctcggcctg agctaccagg acgtcagcct cgatccggcg
     2761 ctgacccccc atcaggtgac actgaccagc cccgacggct cgcagaccac cgtcacctac
     2821 tacagcggtc cggacggcgt ggtcgtcagt ccgctggcgc cggtgctccc cttcgtcagc
     2881 cagagcgcgg acgggccgag cggcacgatc gtgcgcggcg tcggcttctt cggcggcacc
     2941 tacaccgacc tgtccggcgt gtcgccgttg accagcgcgg tcgtcaccga gcagagcagc
     3001 acgcacctcc ccttccactc cagctacctc tacccggcgc agccgtgggc gctcaactac
     3061 ctcggcctgc tgggtggctt cggcccgccg ctcttgcagg tgacgccggc gcagtaccag
     3121 tctgatagcg ccacctcgac caccggcacg ctgcggctgc tgaacgatat gaacttccgc
     3181 ctcttcttca gcggcaacac cgcgacctac cccgcgggcg gcacctaccc gacgagcggc
     3241 gacatccccg cgctggccgc gccgccggcc atctcgcaga tcgcggccac cccggcggcc
     3301 gacggctcga tcaccttcgc ggcccacgtc gccggtgatc cgctggccgg catccagcag
     3361 gtctgggtca cctacaccgt catcccggcg ggcggcggta ccggccgctg gatctcgctc
     3421 gacctgacac agtcgaccgc cgacagcacc ctctggcagg ggagcctgtc gccggccacg
     3481 ctgcggcagc agctcggcac cagcgacctg accaacttcc gcttcatggc ccaggcggcc
     3541 aacggagtcg ggctggtcac gctggcgacc aacttcggca cctactacca ggtcggcagc
     3601 ggcaccccgc cgaacccggc ggccaccacg ctggccttca acggtgcgcc gaccggcggc
     3661 gcctacggca ccaacgcgac cttcagcgcc acgctgtcga gcgccgacgc ggcctgcgtg
     3721 gcggggcaga ccctcttctt cggcatcggc gcgcagcgcg tgccggccac gaccgacgcc
     3781 ggcggccacg ccagcgccac catcccgctg ctcgcctccc cgggcgacgc cgacgcacgc
     3841 gtcacctacg gcggcaacga caactgcaac ctggccagcg ccgagacgcc cttcaccatc
     3901 accaagcagc cgtcgcacct gacgctggtc accagcgacg gtgcgttccc ggtgacggtg
     3961 acgctgaagg acgacaacaa caacccgctg cgccagcgca gcgtctacgt ggccttcacc
     4021 ggcgccgagt tcaacgacgg cgagctggtc accaccgatc aggcaggcac ggccaccatc
     4081 gacagcttcg acctgccggc ggggagcttc accgtcacgg ccagcttcgg ccagccggtc
     4141 acgctccccg acaacacgtc gctcgacctg accgaccagc tctacgccgg cagcagcgcc
     4201 acgggggcgc tcgggggcgg ctcaacgccg gcgagtcaga ccttcctggc ctgggggcgc
     4261 aacaacgagg gtgagctggg caacggcacc acggccgaca gcgccctgcc ggcgccggtc
     4321 gacaccagca acctgggcga cgtgagcgcc ttcgccgccg gcgactacta caccatcgcg
     4381 ctcaagagcg acggcaccgt ctgggcctgg ggcagcaacg gcaaccacta cctgggcgac
     4441 ggcacgaccg cacagcggac gacgccggtc caggtccaga atctgagcag catcatcgcc
     4501 atctccgcgc gtcgcaacca cagtctggcg ctcaagagcg acggcaccgt ctgggcctgg
     4561 ggcgagaacg gcagcggcca gctcggcgac ggcaccacca cctcgccgcc gacgccagtg
     4621 caggtgcagg ggctgccggg caacatcacc gccatcgcgg ccggcggctt cttcagcctg
     4681 gcgctgacgt ccgatggcca catttacgcc tggggcgcca acgactttgg gcagcttggc
     4741 aacggcgtcg gcggcgatac gagcgcccat ccggcgcccg cgctggtgat gatgcccggg
     4801 gtcgtcggcg ccttcaccca gatcaccgcc ggccttgcca acgcctttgc cgtggatcag
     4861 aacggcaacc tctgggcctg gggcgacaac cgggtcggcc tgctggggat cggcagcgcg
     4921 gatagcggcg cccacgccac gccagcgcgc accttcggcc tcagccacgt caccgccatc
     4981 gccgcctact acaaccacac gctggcggtc agcggcggca ccgtctacgc ctggggcaac
     5041 ggcagccccg gcgtgctggg cgacggcacc accaacaacc acgacgcgcc caaccccgtg
     5101 ccgggcctgt cgaacgtcgt cagcgtggcg gtcggcggct accacagcct ggcgctgacc
     5161 gccgacggcc agatgtacgc ctggggcagc aactccgagg atggacgcct cggcgacaat
     5221 gaaaccggga ccggttcgga cgcgctctcg ccggtgccgg tc
//
LOCUS       DASUBI010000499         7691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_369043, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000499 DASUBI010000000
VERSION     DASUBI010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369043"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..742)
                     /locus_tag="VFU72_09610"
     CDS             complement(137..742)
                     /locus_tag="VFU72_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEU5423782.1"
                     /translation="MAGTAKPALLIVDVQNDFTPGGALAVTEGDQVVPLLSDLAARFA
                     ARGYPVYASRDWHPADSRHFQQYGGTWPVHCVQGTPGAAFHPALRLPPNTPVVSAGMS
                     RDDEGYSAFEGRTAEDEPFAERLRRDNVDHLYVGGLATDYCVRASVLDALRHGLTVTL
                     LTDASRGVNLQPGDAERAIEEVRAAGAELATTADVPPLSQG"
     gene            complement(815..1624)
                     /locus_tag="VFU72_09615"
     CDS             complement(815..1624)
                     /locus_tag="VFU72_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5423783.1"
                     /translation="MHRPRYLPAQVHVHVNETVRERARVGLLEFALLVTTFMLYFATR
                     GVAAGKEAVAFGHAHTVMGLEQRLGLFREMGIQTVVLTVPAVTRLLNFVYAYTHLTAL
                     ILFGIWVFCWHHDAYRNVRNTFLVILFTGLAIYIIYPLAPPRLFPYTGFVDTLKLYSG
                     INYDQPSLATLYNPFAAMPSLHVGFAVFVGMGLIRVGKRKLYWIPGIIFPLLMATAVV
                     GTANHYILDALAGTLLTVLAYILVPRVSARLSAWYQVRHEDRTQTWAPTPF"
     gene            1995..3008
                     /locus_tag="VFU72_09620"
     CDS             1995..3008
                     /locus_tag="VFU72_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3435 domain-containing protein"
                     /protein_id="HEU5423784.1"
                     /translation="MSSEPSQLSINAAVEEFFADLRLSPRSKKTYRGALTKFLQRLET
                     QGLDPATAPVEALTEDHVVDFVAALMPEDIRSPEEVSRMRTAQTNLAAVRKFYAYLAT
                     FELHGGLSGDKLRARVAAMMPRFTPPPPDVQTADLDRIVDHVRGLPHENETHRELRRL
                     KIRAMILFLYRSGVRVSELCGLRRRDIDLGSGAGSVYRAKGGKSRTVHFDSETAQALV
                     AYWTARGDSGRGVGALPAFSGRDKAGQPGRAISPRTVEQIVAELCVAAGVENPITPHS
                     FRHGLATELVRRRVRESTVQTILGHASPMTTRIYVHKVALEVAEEYQEAFGEYRRPNG
                     SGV"
     gene            complement(3015..3416)
                     /locus_tag="VFU72_09625"
     CDS             complement(3015..3416)
                     /locus_tag="VFU72_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423785.1"
                     /translation="MRGSAMDRRRLVLIIDHDLELVELLRGLLTDEGFQPLTNLTLEA
                     IAVLPVENRPDLIMLDVQVSHPQPDTALLARLAGSTALAGTPLLLTATDASLLRATVA
                     ALGCPRTDWIAKPFDLDELLRKVEALLTGQP"
     gene            complement(3941..4897)
                     /locus_tag="VFU72_09630"
     CDS             complement(3941..4897)
                     /locus_tag="VFU72_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU5423786.1"
                     /translation="MFLDEMTKTERVAAAVHGDEVDRIPVSFWHHFRPEGSGRKMAQA
                     TIDFFENTYDLDILKIMPDIPYPFPRGAIADPEDWRLLEQLNPRRSHYLTQRATAVRV
                     LREAVGWETPIIMTIYNPLSEAMHFAQTRDLFLEHARQHPSVVHQALATIAENLRRLM
                     RECIDSGADGIFFALQGCTSEVMSQQEYQEFGRPYDLLALQGAIDGWLNVLHVHGTKD
                     LYFQQSLDYPVQVLSWSDRLAGPSLREARTMTSKCLMGGWNEQGALANGPVEQIRAEA
                     EDAIKQTGGRKLILANGCSVPDDTDPKWLEAARALVDELEVP"
     gene            complement(5165..6961)
                     /locus_tag="VFU72_09635"
     CDS             complement(5165..6961)
                     /locus_tag="VFU72_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423787.1"
                     /translation="MESCRLRPARGLRLLSALLVLVLLWALTPAALAAPSASPAPTSD
                     WRIGDVSLAPEAPAAGDVATVRFSVRDTQGAPVPHLSVSAQLRAASTRHGTPPADPIL
                     STLGHETATPGSYEVSVAVNENGSWWLDIAAGDALHQASFSRLLTVDPVKPPTTPAND
                     QDPVFLRGDALGAYYRLDPQTGSVARLSGESVSQAGGAWWITAVHLTPTSDPDPVYGG
                     NWQLQVTFADALRGQRISSVDLGVIRAGVYVGSSDQPAIATAVALAPDGAHAYIYWSR
                     QLGAGWTSWVALVDPRTGVVLRTRLLTGGIAADGEFARLAVSDDSRQLLVAEQVIRGA
                     QAAGYRLTRLDAETLTTRSQLRRTEAADDPLATCLIPYPGPAGALPGSDTTFYSLCTP
                     AGSGAPALVVWDAASGNVIHKVDLSTLAGETASYVDGIASPDGRRFYAVNTVTRQVAE
                     IDLGQGQLLRTVAFGPDPKVQSPSNWNRFFDWLLGMVAPPAAAGVLIEHGVAIAPDGS
                     ALYVVSPAKTGSGGYGDGVWEIDSGSLQATRHLLSGQPVAGVVVALNGRLAVIASDQE
                     SDDSQISVVQPDGQVVISLTLPDNLAATIGAR"
     gene            complement(7103..>7691)
                     /locus_tag="VFU72_09640"
     CDS             complement(7103..>7691)
                     /locus_tag="VFU72_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423788.1"
                     /translation="LAGSALLVVVSYHPFWAGARTFDGVLKQGSLLTSSPASLLSHVA
                     PLGLLRPLFGAAFAAGYLLLLRRVHGLPQRSCAALLLYLATLSFWTKGWYFTWPLPLA
                     ALVGGDVLLVAAVATLGAFVANIFWGWMLPMDWWGMEGPWGLVPLDVTLGLSLYLPWL
                     IGAAWLLRRRGKRVSERRSTILAVASRRILRPRSG"
BASE COUNT         1258 a         2582 c         2736 g         1115 t
ORIGIN      
        1 catcctcacc gacgccaccg acgccaccca cgctgcccca tgccccccgt cgcattgtcg
       61 taggggaggc agggcagaca cagcggtctg cccctacagg tgggcgacac atggcggcca
      121 gtggcgatga ggcgccctac ccctggctca gcggcggcac gtccgcggtc gtcgccaact
      181 cggccccggc cgcacgaacc tcctcgatgg cgcgttcggc gtcgcccggc tggaggttga
      241 cgccccggct ggcgtccgtc agcagcgtga ccgtcaggcc gtgacgcagg gcatccagca
      301 cgctggcgcg cacgcagtag tcggtcgcca gcccgccgac gtagaggtgg tcgacgttgt
      361 ctcgccgcag ccgctcggcg aacggctcgt cctcggcggt gcgaccctcg aaggcggagt
      421 atccctcatc gtcgcggctc atcccggcgc tgaccaccgg cgtgttgggc ggcagccgga
      481 gagcggggtg gaaggccgcg cccggtgttc cctggacgca atgtaccggc caggtgccac
      541 catactgctg gaagtgccgg ctgtcggccg gatgccagtc gcgcgaggca tagacggggt
      601 agcctcgcgc ggcgaagcgg gcggcgaggt cgctcagcag cggcaccacc tggtcgccct
      661 cggtcacggc gagcgcgccg ccgggggtga agtcgttctg cacgtccacg atcaagagcg
      721 ctggtttcgc tgtcccggcc atatcggctc cttgcaatgc cgcggcccga tccagcgggg
      781 ctggatcggg cggatgatca actcgacata acgatcagaa gggcgtgggc gcccaggtct
      841 gcgtgcggtc ctcgtgccgc acctggtacc aggcgctcag gcgcgcgctg acgcgcggca
      901 ccaggatgta cgccagcacc gtcagcagcg ttccggccag cgcatcgagg atgtagtggt
      961 tggccgtgcc gaccaccgcc gtcgccatca ggagcgggaa gatgatgccg gggatccagt
     1021 agagcttccg tttcccgacg cggatcaggc ccatgccgac gaagacggcg aagccgacgt
     1081 gcaggctcgg catggccgcg aaggggttgt agagcgtcgc caggctcggc tgatcgtagt
     1141 tgatcccgct gtagagcttg agcgtgtcga cgaacccggt gtacgggaag agccgcggcg
     1201 gcgccagcgg gtagatgatg tagatagcca gcccggtgaa gagaatcacc agaaacgtgt
     1261 tgcgcacgtt gcgatacgcg tcgtggtgcc agcagaagac ccagataccg aacaggatca
     1321 gcgcggtcag gtgcgtgtag gcgtagacga aattcagcag gcgcgtgacg gccggtacgg
     1381 tgagcacgac cgtctggatg cccatctcgc ggaagaggcc gaggcgctgc tccagcccca
     1441 tcacggtgtg ggcatggccg aaggccaccg cctccttgcc ggcagcgacc ccgcgcgtgg
     1501 cgaagtagag catgaaggtc gtcaccagca gcgcgaactc cagcagcccc acccgtgccc
     1561 gctcgcgcac ggtctcattg acatggacgt gcacctgcgc cgggaggtat cgcggtcgat
     1621 gcatcagtga caggttctcc ttcccgcgtc cggccctggt cgactccgat agttggcgac
     1681 tcccagtccg tagagtcaag caaagcgtag cgggagccgc tccgccatga ccaggagtcc
     1741 gagcatcagg ctgtccaatc ggacagggtg ggcagacgcc ggcacgtggt acgagcccgg
     1801 caccttgcgg ccccaatgca ggccgcgtac cactggctct taacacgatc tgcatgaatt
     1861 atactcccac cgcccacgat ccccggcggg ccgcggcagc gggcacccct tgcggccgcg
     1921 ccgcccggca actatactcg ctgtacgtac gctcctccag gagacgcccc atggcgtcgt
     1981 gacaggaatg gtccatgtcc agcgagccca gccaactgtc catcaacgcg gcggtcgagg
     2041 agttcttcgc cgatctgcgc ctctcgcccc gcagcaagaa gacctatcgc ggcgcgctga
     2101 ccaagttcct gcagcggctg gagacgcagg ggctcgaccc ggccaccgcg ccggtcgagg
     2161 cgctcaccga ggatcacgtg gtcgacttcg tggcggcgct gatgccggag gacattcgct
     2221 cgccggagga agtctcgcgc atgcgcaccg cgcagaccaa cctggcggcg gtgcgcaagt
     2281 tctacgccta tctggccacc ttcgagctgc acggcggtct ctccggcgac aagctgcggg
     2341 cgcgcgtggc ggccatgatg ccacgcttca ccccgccgcc gcccgacgtg cagaccgccg
     2401 acctcgaccg catcgtcgac cacgtgcgcg ggctgccgca cgagaacgag acccatcgcg
     2461 agctgcgccg cctgaagatc cgcgccatga tcctcttcct ctaccgcagc ggcgtgcgtg
     2521 tctccgagct gtgcgggctg cgccgccgcg acatcgacct cggctcgggc gcgggcagtg
     2581 tctaccgggc caagggcggc aagagccgca cggtgcactt cgacagcgag acggcgcagg
     2641 cgctggtcgc ctattggacg gcgcgcggcg acagcgggcg cggcgtcggc gccctgccgg
     2701 ccttcagcgg gcgcgacaag gccggccagc cggggcgtgc gatcagcccg cgcaccgtgg
     2761 agcagatcgt ggccgagctg tgcgtggcgg cgggagtcga gaacccgatc acgccgcact
     2821 ccttccggca cgggctggcg accgaactgg tgcggcggcg ggtgcgcgag tcgaccgtgc
     2881 agacaatcct gggccacgcc tcgccgatga ccacgcgtat ctacgtgcac aaggtggcgc
     2941 tggaggtggc ggaggagtac caggaagcct tcggagagta ccgtcgcccg aacggtagcg
     3001 gagtctgagc gcagtcaggg ctgaccggtc agtaatgcct cgactttgcg cagcagctcg
     3061 tcgagatcga acggtttggc gatccagtca gtgcgcgggc agccgagcgc cgcgacggta
     3121 gcgcgcagca ggctggcgtc ggtcgcggtc aagagaagcg gcgtgccggc cagggccgtg
     3181 ctgccggcga gccgcgcgag cagcgcggta tcgggctggg ggtggctgac ctgcacgtcg
     3241 agcatgatca gatcggggcg attctcgacg ggcaggacgg cgatggcctc gagcgtcagg
     3301 ttggtcagcg gctggaagcc ctcatcggtc agcagcccgc gcagcagctc aaccagctcg
     3361 aggtcgtggt cgatgatcag aactaaccgg cgacgatcca tcgcgcttcc tcgcatctcg
     3421 cctcctgccg gggcatgggc cgcgtgcact ggccggcgaa tacgacttcg ccgggccgcg
     3481 gagcgcagga atgagaggta gggtagatcg gacgtggggt agacccggcg acgcagcggg
     3541 gcgcaggctc ccgcgtcgca ctgaaggcgt ggccgtcagt gcgcgtctac cgtcacgacg
     3601 gttgggaatg tgccgcacag ccgcgcacac gacgtgcccg gcatagtgtg aagcagacca
     3661 gctcacccgc atggtcatca tggatgcgtg atcgggattt gtcaagggcg gtatgtgcgt
     3721 aggacgacgg cgcgttcgca ctgccagtcc tgccataggg cggctaccgg tgctcattga
     3781 ggactcgcgc cgtaggggaa ttgggcggac acgaggttga tggtcattga cgaattgagt
     3841 tggccgtcgc ggcgggatcg cgcggcgacg gccaactcaa ttcgtcaatg accatccccc
     3901 ggcgcgagct agtacccgcg ctggtctttc ccagcggcct ctacggcacc tccagctcat
     3961 ccaccagcgc ccgcgccgcc tccagccact tggggtcggt gtcgtccggc accgagcagc
     4021 cattggccag gatcagcttg cgcccgccgg tctgcttgat cgcgtcctcg gcctcggcgc
     4081 ggatctgctc caccgggccg ttggccagcg ccccctgctc gttccagccg cccatcaggc
     4141 acttgctggt catggtgcgc gcctcacgca ggctggggcc ggccaggcga tcgctccagc
     4201 tcaacacctg caccgggtag tcgagcgact gctggaaata cagatccttg gtgccgtgga
     4261 cgtgcagcac gttgagccag ccgtcgatcg cgccctgcag cgccagcagg tcgtaggggc
     4321 ggccgaactc ctgatattcc tgctgcgaca tcacctccga ggtgcagccc tgcagcgcga
     4381 agaagatgcc gtcggcgccg ctgtcgatgc actcgcgcat caagcggcgc aggttctcgg
     4441 cgatggtcgc cagcgcctgg tggaccacgc tgggatgctg gcgggcgtgc tccagaaaga
     4501 gatcacgagt ctgggcgaag tgcatcgcct cggagagcgg gttgtagatt gtcataatga
     4561 tcggcgtttc ccagccgacc gcctcgcgca gcacgcgcac ggcggtggcg cgctgggtca
     4621 ggtagtgcga gcggcgcggg ttaagctgct cgagcaagcg ccaatcctcg ggatcggcga
     4681 tcgcgccgcg cgggaagggg taggggatat ccggcatgat cttgaggata tcgaggtcgt
     4741 aggtgttctc gaagaagtcg atcgtcgcct gggccatctt gcgcccggag ccttccggcc
     4801 ggaagtgatg ccagaagctg acggggatgc gatcgacctc gtcgccgtgc accgccgccg
     4861 ccacccgctc ggtcttggtc atctcatcca gaaacaccgc tcggctccct tcctgctccg
     4921 ctgtctccac cgttcgcgcg gcatcttagc ataggcgccc ggtctacccg ggtggagggt
     4981 gcgggagggc ttgcgccccg gttggggaac cggggcgcgg aacagaggaa gggaagggga
     5041 ttgggaagag taagaggtgc gcgggcgcga tgtggggggc atcgcccctg ctatccctta
     5101 aaacaccgcc gcgggcgaaa acgttacacg agagtgaggg tgagggcgaa ggtgaggttg
     5161 gcgctcagcg cgcgccgatg gtggccgcga ggttgtccgg caaggtcagc gagatgacga
     5221 cctgaccgtc cggctgcacc acgctgatct ggctgtcgtc gctctcctgg tcgctggcga
     5281 tgaccgccag gcggccgttc agcgccacca ccacgccggc caccggctga ccgctgagca
     5341 agtggcgcgt ggcctgcagc gagccgctgt cgatctccca gacgccgtcg ccgtaaccgc
     5401 cggagccggt cttggccggc gagacgacgt agagcgccga gccgtcgggg gcgatcgcga
     5461 ccccgtgctc gatcaagacg ccagcggcgg ccggcggcgc caccatgccg agcagccaat
     5521 cgaagaagcg gttccagttg gagggcgact gtacctttgg gtcgggcccg aaggcgacgg
     5581 tgcgcaggag ctgtccctgg ccgaggtcga tctcggccac ctggcgcgtg accgtgttga
     5641 ccgcgtagaa gcgccggcca tcgggcgagg cgatgccgtc gacatacgag gccgtctcgc
     5701 cggccagcgt gctgaggtcc accttgtgga tgacgttgcc gctggcggca tcccagacga
     5761 ccagcgccgg agcaccactc ccggccgggg tgcagaggct gtagaaggtc gtgtcgctgc
     5821 cgggcaacgc gccggccggg ccggggtagg gaatcaggca ggtggcgagc ggatcgtcgg
     5881 ccgcctcggt gcgccgcagt tgcgagcgtg tcgtcagcgt ctcggcgtcg agccgcgtca
     5941 ggcgatagcc ggccgcctgg gcgccccgga tcacctgctc ggccaccagc agttgccggc
     6001 tatcgtccga gaccgccagc cgcgcgaatt cgccgtccgc ggcgataccg ccggtcagca
     6061 gtcgcgtgcg cagcaccacg ccggtacgcg ggtcgaccag ggccacccag gaggtccagc
     6121 cggcgccgag ctggcgcgac caatagatat aggcgtgggc gccgtcgggc gccagcgcca
     6181 cggcggtggc gatagccggc tggtcgctgc tgccgacgta gacccccgcc cggatcacgc
     6241 ccaggtcaac gctgctgatg cgctgcccgc gcagcgcatc ggcgaaggtg acctgcaact
     6301 gccaattgcc gccgtagacc ggatcggggt cgctggtggg cgtcaggtgg accgccgtga
     6361 tccaccaggc gccgccggcc tggctgaccg actcgccgct cagccgcgcc acgctgccgg
     6421 tctgagggtc gagccggtag taggccccca gggcatcgcc gcgcaggaag accggatcct
     6481 ggtcgttggc cggcgtcgtc ggcggcttga ccggatcgac cgtcagcagc cgggagaagc
     6541 tggcctggtg cagggcgtcg ccggccgcga tatcgagcca ccacgagccg ttctcgttga
     6601 cggccaccga aacctcgtag ctgccggggg tggctgtctc atggccgagc gtgctgagga
     6661 tggggtcggc cggcggggtg ccatggcggg tgctggccgc gcgcaattgc gcgctgaccg
     6721 acaggtgcgg caccggcgcg ccctgggtat cgcgcacgct gaagcggacc gtggcgacat
     6781 cgccggcggc gggcgcctcg ggcgcgagcg agacatcgcc gatgcgccag tcgctggtcg
     6841 gcgccggcga ggcgctcggc gcggccaggg ccgccggcgt cagcgcccag aggagcacca
     6901 gcaccagcag ggcgctcagc aggcgcaggc cgcgcgccgg acgaagcctg caagattcca
     6961 ccggatgcgt gcaggtcggc cgcgtcatag ggctagtgta cccgctcgcc gggagaatgg
     7021 tagggggtgg acacgggacg gtactccccc gcgaaagatg cccgtcgatg tgccgtcgtc
     7081 gttcgccaca ccccttacct tgtcatcctg agcgcggtcg aaggatccgt cttgaggcga
     7141 cagccaatat ggtcgaccgc ctctcgctaa ctcgcttccc acgtcttcgc aacagccacg
     7201 ccgcgccgat gagccagggg aggtagagcg agaggccaag cgtcacgtcc agcggcacca
     7261 acccccacgg cccttccata ccccaccagt ccatcggcag catccagccc cagaaaatgt
     7321 tggccacaaa ggcgcccagc gtggccaccg ccgcgaccag cagcacgtcg ccgcccacca
     7381 gcgcggccag cggcagcggc caggtgaagt accagccctt ggtccagaag gagagcgtcg
     7441 ccaggtaaag caggagggcg gcgcacgagc gctggggcag cccgtgcacg cgccgcagca
     7501 gcaggagata gccggcggcg aaggcggcac cgaagagcgg ccgcaggagc cccagcggcg
     7561 cgacgtggct gagcaggctg gccggcgacg aggtcagcag gctcccctgc ttcagcacgc
     7621 cgtcgaaggt gcgcgcgccg gcccagaagg gatgatacga gacgacgacc agcagcgccg
     7681 agccggccag c
//
LOCUS       DASUBI010000500         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_129795, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000500 DASUBI010000000
VERSION     DASUBI010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129795"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>2071
                     /locus_tag="VFU72_09645"
     CDS             39..>2071
                     /locus_tag="VFU72_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423789.1"
                     /translation="MSTPRELRRLAIGEAGWGPALVLAAVAVVAAFISIAGPRLVVTA
                     DNTATKQAIAQMPVLDSGIFTTATPSATLPSGVLSAAKIDKIGRLLVAGLPRRQDFHP
                     GQTWASVLMPSAIVANPAPSAVTIRPPSMEIAYRSGLASRCVVVAGSLPGTARGSGGS
                     PPRASTAGPIRSGSGGVRSVTLAVVVTQANAARFRLAVGSVMDLDAATIGDPSVRLKV
                     TGIIRPKVPGSTFWQAINSLRAPALEPIPGSAIPGAVYYLGGAFVGPSELGALSTAYQ
                     TSNEQVTWYIPMRMSLTAAEVPRLESAMESFVASPVAIKAEEQVTGAPLPGTENTADL
                     AQGLSAFTAQWHSVTTTDSLLLVGLFVAGAMLLFICSGLAIESYRPELILLRVRGGSL
                     RQLARRMLARSCLLAGLPLAAGGVLAVVVVPGSGTAASWVLGGLTALVAIGGLPVLAM
                     LAHRERRLADLGRRDELVVERPRLRRLVAELAIVVVCAAAVADLRLRGAGTSSTARYL
                     SASAVLVAAVVGLVVNRAYRGPLRAAAKVAGVMKGPVGMIGLTRAALARTASVAPALV
                     LMLTLTLVTFTGMVISAVSAGQVSASWAQVGADVQVNAPGLTGLRLTGVTAGQLRDLA
                     RISGVRHVTSVYTAQGTSTLAVEVTTAGKTGTLTGLAVVTPASYGAVAADTPWP"
BASE COUNT          273 a          717 c          750 g          331 t
ORIGIN      
        1 accagcggcg ccgccagggt caggatcgag gaagagacgt gagcaccccg cgtgagctgc
       61 ggcggctggc gatcggagaa gccggatggg ggcccgctct ggtgctggcg gccgtcgccg
      121 tcgtcgcggc ctttatctcg atcgcgggcc cccgcctggt ggtaacggcg gacaataccg
      181 cgaccaagca agcgatcgcg cagatgcctg tgctcgactc gggcatcttc accacggcca
      241 ccccgtcggc cacgctcccg agcggcgtgc tttctgccgc caagattgac aaaatcggcc
      301 gcttgctggt ggccggattg ccgaggcggc aggacttcca tcccggccag acctgggcca
      361 gcgtcctcat gccgtcggcc atcgtcgcga acccagcacc ctcggccgtg accatccggc
      421 cgcccagcat ggagatcgct taccgcagcg ggctcgcgtc gcggtgcgtt gtggtcgccg
      481 gctcattgcc tggcaccgcg aggggttccg gggggtcgcc cccccgggcc agcaccgccg
      541 gcccgatcag gtcaggcagc ggcggcgttc gatcggtgac gctggcagtc gttgtcacgc
      601 aggccaacgc cgcgaggttc aggctggctg tcgggtcggt gatggacctc gacgccgcga
      661 ccatcggcga cccgagcgtc aggctcaagg tgaccggcat catcaggccg aaggtgcctg
      721 gttcgacgtt ctggcaggcc attaactcgc tcagggcccc cgcgctcgag ccaatccccg
      781 gcagcgcgat accaggcgcg gtgtactacc ttggcggcgc gttcgtcggg cccagtgagc
      841 tcggcgcgct gtccacggcc taccagacct cgaatgagca ggtcacctgg tacattccga
      901 tgcgcatgag tctcaccgcg gccgaagtgc caaggcttga atcggcgatg gagtccttcg
      961 tcgcatcgcc ggtggcgatc aaggcagaag agcaggtgac gggcgccccg ctgcctggga
     1021 cagagaacac cgccgacctc gcgcaaggac tgtcggcctt caccgctcag tggcacagcg
     1081 ttaccacgac cgactcgctg ctgctcgtgg gtctgttcgt ggccggcgcg atgctgttgt
     1141 tcatctgctc ggggctggcc atcgagtcct accggcccga gctgatactg ctgcgagtgc
     1201 gcggcggcag cctgcgccag ctagcccgcc ggatgctggc cagatcctgc ctgcttgcgg
     1261 ggctgccgct cgccgctggc ggcgttctgg ccgtggtcgt ggtgccgggc agcggcaccg
     1321 cggcgagctg ggtgctcggt ggcctgaccg ccctggtggc gatcggcggc ctgcccgtgc
     1381 tcgcgatgct cgcgcatcgc gagcggcggc tcgccgacct cggccggcgc gatgagctgg
     1441 tcgtcgaacg tccgagactg cggcgcctgg tcgccgagct cgcgatcgtg gtcgtctgcg
     1501 ccgccgcggt cgccgacctg cggctgcgcg gagcgggaac gtcgagtacg gccaggtacc
     1561 tgagtgcgag tgcggtgctc gtggccgcgg tggtcggcct cgtcgtgaac cgggcctacc
     1621 gcggcccgct gcgcgcggcg gccaaggtcg ccggcgtgat gaagggcccg gtcggcatga
     1681 tcggcctgac cagggcggcg ctggccagga cagcttcggt tgcgcccgcg ctcgtgctga
     1741 tgctcacgtt gacgcttgtg acgttcaccg gcatggtcat ctcggcggtc tcggcgggcc
     1801 aggtgagcgc gtcctgggcg caggtcggcg ccgacgtcca ggtgaacgcg cccgggctga
     1861 ccggcttgcg gctgaccggg gtgaccgccg gccagttgcg ggatctggcc aggatcagcg
     1921 gagtccgcca cgtcacgtcg gtctacaccg cgcagggcac gtcgacgctg gctgtcgagg
     1981 tcaccaccgc cggcaagacc ggcacgctga ctgggctcgc cgtcgtgacg ccggcctcct
     2041 acggagccgt tgccgccgac acgccgtggc c
//
LOCUS       DASUBI010000501         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_253633, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000501 DASUBI010000000
VERSION     DASUBI010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253633"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFU72_09650"
     CDS             complement(<1..676)
                     /locus_tag="VFU72_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5423790.1"
                     /translation="MSEIRIVELEKRYGAVAALRGISATVRSGELLALLGPSGCGKTT
                     TLQLLAGFAAPDGGQIWLGDRLLSSPAQTVPPEKRNMSLVFQSYALWPHMTVADNVAF
                     GLQLRKLPRDEIRRRVARMLEVVNLGGYQRRYPHELSGGQQQRVALARALVVEPDTLL
                     LDEPLSNLDANLREQMRFEIRRIHDETGITTVYVTHDQAEAMVIADRIAVMHAGLIEQ
                     IGAPAEI"
     gene            complement(683..1057)
                     /gene="queF"
                     /locus_tag="VFU72_09655"
     CDS             complement(683..1057)
                     /gene="queF"
                     /locus_tag="VFU72_09655"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU5423791.1"
                     /translation="MPTQPSKALETFPNPNPERDYEIAISTPEFTCVCPMTGQPDFAT
                     ISIRYVPDQRCVELKSLKLYLWSYRDEGAFHEQVTNRILDDLIAAIAPRRATVEGDFN
                     VRGGLHTVVTASYEAPMSKLQP"
     gene            1197..1334
                     /locus_tag="VFU72_09660"
     CDS             1197..1334
                     /locus_tag="VFU72_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423792.1"
                     /translation="MQQKHPLTDREKRIIGMVALACFIVLVIWLAAVVLWLPVPAQGL
                     V"
     gene            complement(1425..2660)
                     /locus_tag="VFU72_09665"
     CDS             complement(1425..2660)
                     /locus_tag="VFU72_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEU5423793.1"
                     /translation="MKGWRRGALLLAGLLALMIGPLGALAAPPAGLPEPAANVVPVCP
                     GPAQPGTARCHALLRTDIGGIPDRGSPNFTNPPGYHPADLQAAYNLGTAQSNFPVAMS
                     GGAVLWSQQPAIAIVDAYDDPYAEQDLGVYRSTFGLPTCTTANGCFKKVNQNGGTRYP
                     RANGGWSQEISLDLDMASAVCPYCRIILVEAGSNSLGNLFAAVDQAVQQGAVAISNSY
                     GAGEFSSEASYDSHFKHPGIAITVSSGDNGYGVEYPAASPYVTAVGGTSLTGSGSSWS
                     ETVWSGAGSGCSAYETKPSWQTDAGCGKRTVADVAAVANPNTGVAVYDSYRYAGMSGW
                     LVFGGTSVAAPIIAGVNALAGNTAVFGNASYAYSHAAALNDVTSGSNGSCGGSYLCTA
                     KPGYDGPTGNGTPNGTGGF"
BASE COUNT          435 a          945 c         1096 g          490 t
ORIGIN      
        1 agatctcggc cggcgcgccg atctgctcga tcagcccggc gtgcatcacc gcgatgcggt
       61 cggcgatcac catggcctcg gcctgatcgt gcgtcacgta gacggtggtg atgccggtct
      121 cgtcgtggat gcggcggatc tcgaagcgca tctgctcgcg caggttggcg tcgaggttag
      181 agagcggctc gtcgagcagg agtgtgtccg gctcgaccac cagtgcccgc gccagcgcca
      241 cgcgctgctg ctgcccgccg gagagctcgt gcgggtagcg ccgctggtag ccgcccaggt
      301 tgaccacctc cagcatgcgc gccacgcgcc ggcgaatctc gtcccgcggg agcttccgca
      361 gttgcagccc gaaggcgaca ttgtcggcga ccgtcatgtg cggccagagg gcgtaagact
      421 ggaagaccag cgacatgttg cgcttctcgg gcggcacggt ctgcgcgggg ctggagagga
      481 gccggtcgcc cagccagatc tggccgccgt cgggcgcggc gaacccggcc aggagctgga
      541 gggtggtcgt cttgccgcag ccggaggggc cgagcagcgc cagcagctcg cccgagcgca
      601 cggtggcgct gatgccccgc agcgcggcca cggcgccgta acgcttctca agctcgacga
      661 tgcggatctc gctcacggcg cgctagggct gcagcttgga catcggcgcc tcgtagctgg
      721 cggtgacgac ggtgtgcagc ccgccgcgca cgttgaagtc gccctcgacc gtggcgcggc
      781 gcggggcgat ggccgcgatc aggtcgtcga ggatgcggtt ggtgacctgc tcgtggaagg
      841 cgccctcgtc gcgatacgac cagaggtaga gcttgagcga cttcagctcg acgcagcgct
      901 ggtcgggcac gtagcggatg ctgatggtgg cgaagtcggg ctggccggtc atcgggcaga
      961 cgcaggtgaa ctccggcgta gagatcgcga tctcgtagtc gcgctcgggg ttggggttgg
     1021 ggaaggtctc gagcgccttg ctcggctggg tcggcatcgt ctcctccgtc tatcgcggtc
     1081 ccggcgcggg atctgtcggg agaatccgcg cggcgattga agtatacgcg cccgtgtacg
     1141 ccggtcgggg aagtgggtta gaatgcgcgg gagaagcgga aggagttgag ccgaggatgc
     1201 agcagaaaca cccgctgacc gaccgtgaga agcggatcat cggtatggtc gccctggcgt
     1261 gcttcatcgt gctggtgatc tggctggccg cggtcgtcct ctggctgccg gttccggctc
     1321 aggggctcgt ttagcagcag cgtccatcac gccgtgcagg cgaggtttcg ggaagaagag
     1381 cccgaggggg atacccctcg ggcttgtcct gtctcgcggc cggtttagaa gccgcccgtg
     1441 ccgttcggcg tgccgttgcc ggtcgggccg tcgtagccgg gcttcgcggt gcagaggtag
     1501 ctgcccccgc agctcccgtt gctgccgctg gtcacgtcgt tgagcgccgc ggcgtggctg
     1561 taggcgtacg aggcattccc gaagaccgcc gtattgccgg ccagcgcgtt gaccccggcg
     1621 atgatcggcg cggccacgct ggtgccgccg aagaccagcc agccgctcat acccgcgtag
     1681 cggtagctgt cgtagaccgc cacgccggtg ttcggattgg ccaccgccgc cacgtcggct
     1741 accgtccgct tgccgcaacc cgcgtcggtc tgccagctcg gcttggtctc gtaggcgctg
     1801 cagccgctgc cggcgccgct ccagaccgtc tcgctccaac tactgccgga gccggtcagc
     1861 gaggtgccgc cgacggcggt gacgtagggc gaggccgcgg gatactcgac accgtagccg
     1921 ttgtcgccgg agctgacggt gatcgcgatg cccgggtgct tgaagtggga gtcgtagctc
     1981 gcctcgctgc tgaactcacc ggcgccgtag ctgttgctga tggcgacggc gccctgctgg
     2041 accgcctgat cgaccgcggc aaacaggttg ccgaggctgt tgctgccggc ttcgaccaga
     2101 atgatgcggc agtaggggca aacggccgag gccatgtcga gatcgagcga gatctcctgc
     2161 gaccagccgc cgttggcgcg cgggtagcgg gtgccgccgt tctggttgac cttcttgaag
     2221 cagccgttgg ccgtggtgca ggtgggcagc ccgaaggttg agcgatagac ccccaggtct
     2281 tgctcggcgt aggggtcatc ataggcgtcg acgatggcga tcgccggctg ctggctccag
     2341 agcaccgcgc cgccgctcat cgcgaccggg aagttactct gcgccgttcc gaggttgtag
     2401 gccgcctgca gatcggccgg gtgatagccg ggcgggttgg tgaagttcgg gctgccccga
     2461 tcggggattc caccgatatc ggtacgcagg agcgcgtggc agcgcgcggt gcccggctgc
     2521 gccggaccgg ggcagacggg gacgacgttg gccgccggct ccggcagacc ggcaggcggt
     2581 gcggccagcg ctcccagcgg tccaatcatg agcgcgagta acccagccag cagcagtgcg
     2641 ccgcggcgcc agcctttcac cctgtggctc ccttcccatg ccgaagcgtg gcatctaccc
     2701 cgaccccggc agtattcact tccgaagaaa cgggcgaagt ctatcacggc aagaggttct
     2761 gtcaaggtgg cgcaatgggg attttaggcg ggaaatcaca cggctggacg cgcgcacacg
     2821 acggtacgga tgcaggaccc ggaaacgtgg cagccgcggt cttgccgcgg ctgtcagcgt
     2881 atctcgaacg ggatcagcgc gccgggcgca ggcggcgcag gacgcggcgc agcgattgga
     2941 cgcgcggctc gccggtcagc tccggg
//
LOCUS       DASUBI010000502         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_917804, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000502 DASUBI010000000
VERSION     DASUBI010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917804"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VFU72_09670"
     CDS             complement(<1..1556)
                     /locus_tag="VFU72_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEU5423794.1"
                     /translation="MLRQADVRLVTLTGPGGVGKTRLAIQIARDLAAAFADGVGFVPL
                     AAVREPALALPSIALALGIREAPDLSLADQLATILRERQLLLVLDNFEQIVDAAPQVT
                     GLLERCPRLTVLTTSRVRLRLTGEQTFPVPPLALPNLTPSASPEDVAHAEAVRLFVAR
                     ASAVDPSFSLDGATAPAVAQICRRLDGLPLAIELAAARGDVLPPRALLARLVRSLSVL
                     TDGPRDAPPRLRSLRDAIAWSYDLLLPEAPARFRRWSVFVGGFTLQAAAAVGTDDEML
                     VEGIAHLVGASLLRLEAGPDGEPRYLMLETIREFGLERLAASGDAEPVRRRHAEHFAR
                     FAEPMISSALAGPDPALTLARMDADRDNLRAALAWADEQGDVALLLRLTAGLKVHWAM
                     RGRLREARAWLDRALALASDASDAPIDLRAGALLAAGWIAWDQRDNERAAALAQQALA
                     LFRDQGDSSGQAEGLALLSIAATDLGQFEQAQALHEELFRLLPDLGDPDESFWTAVTL
                     QEAARLSLLVG"
     gene            complement(1854..2609)
                     /locus_tag="VFU72_09675"
     CDS             complement(1854..2609)
                     /locus_tag="VFU72_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEU5423795.1"
                     /translation="MKSRILVALALLALGVGLAAPALAAAPHPSDQPRHTLRNGTSTN
                     WSGYAVDAQNVTDVRGTWVVPAVDCAGVQDQAASSVWVGIDGDTNNTVEQTGTDQLCD
                     GQHAVSDAWIEMYPSMGRTLIKLPVHPGDRISAEVQWLKTQVFSLRLTNETTGQRFQT
                     QTGHHAAKASAEWIVECPGTPGVLPLADFGTARFSNASATIGGTTGPINAKPWADAVE
                     PITMVAADETTVRAVPSGLRQSGSGFSVTWQHR"
     gene            complement(2833..>3597)
                     /locus_tag="VFU72_09680"
     CDS             complement(2833..>3597)
                     /locus_tag="VFU72_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423796.1"
                     /translation="MSIDATALDLPPPYIALQAVHAIQSLLAYPVLLLLLSWLYRVGA
                     ARLPKVRTWSGRQLQRFGRVALLVVNLLIVLPLILAAAGAASSPALVQTTSALSEVVS
                     LMQWAGLALLVYVVWLSLGPGTLLLAALQRRRILPIVLLSALYFCGALVDTAGRARAN
                     AEHLMTGESDTSMAVTFTLAAGVAPLPRADLLLVAIRNGHYFVVERQPNPPSLTPIAF
                     AIPFRAVDAVHMERVNPAAPAAHGIIVDFFGSTPVP"
BASE COUNT          596 a         1214 c         1303 g          484 t
ORIGIN      
        1 ccgaccagca acgacagtcg agcggcctcc tgcagcgtga cggcggtcca gaacgattcg
       61 tccgggtctc cgaggtccgg gaggagccga aagagctctt catgcagcgc ctgggcttgc
      121 tcgaactggc cgaggtccgt ggcggcgatg ctgagcagcg ccaacccctc cgcctgtccg
      181 gacgagtcac cctgatcgcg aaacagcgcc agcgcctgct gcgccagcgc tgccgcgcgc
      241 tcgttgtcgc gctgatccca agcgatccag ccggccgcca gcagcgcccc ggctcgcaga
      301 tcgattggcg cgtccgaggc atccgaggcg agggccagcg cccggtcgag ccacgccctg
      361 gcttctctga gccggccgcg catcgcccaa tgcaccttca accccgccgt cagccggaga
      421 aggagcgcga cgtcgccctg ctcgtcagcc caggcgagcg cggcgcgtag gttgtcccga
      481 tcggcgtcca tgcgagccag cgtgagcgct gggtcgggcc ccgcaagcgc ggacgagatc
      541 atcggttcgg cgaagcgggc aaaatgctcg gcgtgccgac ggcgaacggg ctcggcgtcg
      601 ccgctcgccg ccagccgttc caacccgaac tcgcggatcg tctccagcat caggtagcgc
      661 ggctcgccgt ctggtccggc ctccagccgc aacaggctcg cgccaaccag gtgcgcgatc
      721 ccctccacaa gcatctcgtc gtccgtgccg acggcggccg ccgcctgcaa cgtgaagccg
      781 ccaacgaaca cgctccaccg ccggaagcgg gccggcgctt ccggcaggag gaggtcgtag
      841 gaccaggcga tcgcgtcgcg gagcgaacgc agccgcgggg gcgcgtcgcg cgggccgtcg
      901 gtgagcaccg acagcgagcg gacgagtcga gccagcagcg ctctgggggg caacacgtcg
      961 ccacgcgcgg cagccagttc gatggcaagc ggcaacccat ccaggcgacg gcaaatctgc
     1021 gccaccgcgg gagcggtggc gccgtcgagt gagaacgacg gatcgacggc gctcgcccgg
     1081 gcgacgaaga gcctgaccgc ctccgcgtgc gccacgtcct ccggcgacgc cgacggcgtg
     1141 aggttcggca gggctaatgg cggcacgggg aaggtctgct cccccgtgag gcgcaaccgc
     1201 acccggctgg ttgtcaggac tgtgaggcgc ggacagcgct ccagcagtcc cgtgacctgc
     1261 ggcgccgcgt cgacaatctg ctcgaagttg tcgaggacga ggagcagctg gcgctcacgg
     1321 aggatggtgg ccaattggtc cgcgagggaa aggtcgggcg cttcgcggat accgagcgca
     1381 agcgcaatcg acgggagcgc cagcgccggc tcgcgcaccg ccgccagcgg gacgaacccg
     1441 actccgtccg cgaaggcggc agccagatct cgcgcgatct ggatggccag ccgggtcttg
     1501 ccgacgccgc ccggtcccgt cagcgtgacg aggcggacgt ccgcctggcg cagcaacgcc
     1561 gcgaccgcgg cgacctccgc ctcccgcccc accagcgagg tgagcggccg ggggagcgtg
     1621 gcctccggcg cccagcgcgg ctcgggagcc ggtcgcagcg gcacgggatc gtggtcgagc
     1681 ggggtggagg accgcggcgt ggacatggac gtctcgtctc ggacgttgtt gagttgtcgc
     1741 aggtggtagg atgcgcaacc ggcgcgatgc cgtcaactgt ccacgggctc tcgggctctc
     1801 gcgcgccacg tgcgggaagt cgcaaatgtt tcaacgcgcc ggcgtcggcg cgcttagcgg
     1861 tgttgccagg tcacgctgaa gccgctgccg ctctggcgca aacccgaggg cacagcgcgc
     1921 acggtcgtct cgtcggcggc gaccatcgtg atcggctcca cggcatcggc ccacggtttc
     1981 gcgttgatgg gcccggtcgt gccgccgatc gtggcgctgg cgttgctgaa gcgggccgtg
     2041 ccgaaatccg ccagcggcaa gacgccgggc gtgccggggc actccacgat ccattcggcc
     2101 gaggctttgg cggcgtggtg gccggtctgg gtctggaagc gctggccggt cgtctcgttc
     2161 gtcagccgca gcgaaaagac ttgcgtcttg agccactgga cctcggcgct gatccgatcg
     2221 cccggatgga ccgggagctt gatcaaggtg cggcccatcg agggatacat ctcgatccag
     2281 gcgtcggaga cggcgtgctg gccgtcgcac agttggtcgg tcccggtctg ctcgacggta
     2341 ttgttcgtgt cgccgtcgat gccgacccaa acgctcgagg cagcttgatc ctggacgccc
     2401 gcgcaatcga cggccgggac cacccaggtt ccccgcacgt ccgtcacgtt ttgggcatcg
     2461 acggcgtagc cggaccagtt ggtcgaggtt ccattacgca aggtgtgacg gggctggtcg
     2521 ctggggtgcg gcgcggcggc gagcgccggc gcggccaggc cgacgccgag cgccagcagc
     2581 gcgagcgcga ccaagatccg ggatttcatg gcggtctccg atcctcttac gccgcatcgc
     2641 ccgtagcccg cgccgggtcg tccggtcggc aaggtcaggc tcgggcgccg gcgacaagcg
     2701 ccaaaccgcg cacagtcgcg acgtgattga tgctagcgcc atggcatgag cgagacgtga
     2761 aggcaggatg acgggttgct ttgagccgag cagcccggat gccgccccac aagcagctgg
     2821 tatggaatgt cgttacggga ctggggtgga cccgaaaaaa tcgacgatga tgccgtgggc
     2881 ggcgggagcg gccggattga cccgctccat gtggaccgca tcgacggcgc ggaacggaat
     2941 ggcaaaggcg atcggggtca ggctcggcgg attcggctgg cgctcgacca cgaagtaatg
     3001 gccgttgcga atcgcgacga ggagcaagtc cgcgcgcggc aggggcgcca cgccggcggc
     3061 gagggtgaag gtgacggcca tgctggtgtc cgattcgccg gtcatcagat gctccgcgtt
     3121 cgccctggcg cggccggccg tatcgaccag cgccccgcag aaatagagcg cggacaacaa
     3181 cacgatcggc aagattcggc gccgttgcag cgcggcgagc aaaagcgtgc cgggacctaa
     3241 actcagccac accacgtaga ccagcagggc gagcccggcc cactgcatca agctgaccac
     3301 ctcgctgagc gccgaggtgg tctggacgag cgcaggcgag gacgccgcgc ccgccgctgc
     3361 caggatgagg gggagcacaa tgaggaggtt gaccaccagc agggccaccc gcccaaagcg
     3421 ctggagttgg cggccgctcc acgtccgcac cttcggcaag cgcgcggcgc cgacccggta
     3481 cagccaagag agcagcagca acagcaccgg gtacgccagg aggctctgga tggcatgaac
     3541 cgcctgcaag gcgatatagg gcgggggcag atcgagcgcg gtcgcgtcga tcgacat
//
LOCUS       DASUBI010000503         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_703194, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000503 DASUBI010000000
VERSION     DASUBI010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703194"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFU72_09685"
     CDS             complement(<1..304)
                     /locus_tag="VFU72_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU5423797.1"
                     /translation="MIRLRKQAELQPIQGSWKIAEVIKRHPELVDELAAINPAFRALQ
                     NPIARRVQGSLVTVSQAARIGGVDEGMLLRRLNTAIGLEADGAPVSDGAGRQRGRCP"
     gene            complement(308..625)
                     /locus_tag="VFU72_09690"
     CDS             complement(308..625)
                     /locus_tag="VFU72_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5423798.1"
                     /translation="MSSLLLSEAAVRAALYEVYDPELGINVVDLGLVYGVDITGSNVT
                     VTMTLTTPGCPLHDSLTAAVDEAVRVFVPSVEDVAVNLVWEPAWSPEMITEAGRDELG
                     WWG"
     gene            complement(622..1125)
                     /locus_tag="VFU72_09695"
     CDS             complement(622..1125)
                     /locus_tag="VFU72_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5423799.1"
                     /translation="MEPRIHYHKVAPGATEGLETLEHYVRHSGLEPALLELVRMRTSQ
                     INGCAYCLDMHSKVAMAHGESAQRLFTLDAWRETPFFSERERAALAWTEAVTLVSVDH
                     VPDEVYEQARGQFSAEELVKLTMAIIAINGWNRLAIPFRSEAGTFHLPGRPAASGESP
                     AREERAR"
     gene            complement(1138..1398)
                     /locus_tag="VFU72_09700"
     CDS             complement(1138..1398)
                     /locus_tag="VFU72_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU5423800.1"
                     /translation="MTYVIAQPCIGVKDGSCVEVCPVDCIHTDDAAEQYYIDPDECID
                     CGVCAEVCPVEAIFFEDDLPEEWTNFLLVNKQYFAKAAAERG"
     gene            complement(1420..1641)
                     /locus_tag="VFU72_09705"
     CDS             complement(1420..1641)
                     /locus_tag="VFU72_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU5423801.1"
                     /translation="MQVTQESSIDEIIEQHPRAANAFIRRRMHCVGCPLARFESLRDV
                     CLVYRQPLDSMLAEIRALAAEQPDTAESA"
     gene            complement(1825..2571)
                     /locus_tag="VFU72_09710"
     CDS             complement(1825..2571)
                     /locus_tag="VFU72_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5423802.1"
                     /translation="MTTHDDNQAAWPIPERAAFLASLRLFRDLLPETLRAIAGRMRVT
                     PVADGDFVFVEGARADAINLLASGRIKVIHETDDGREVILRLIQPGEIFGGAGIWGAD
                     SYPASAIALEDAVVLRLPARELTTLAKKHPEFALALVAELAARLREAEARIQELQTER
                     VERRIARALLRLTDKLGRKTERGIEIDLPLSRQDLAELAGTTLSTASRTLSAWQQQGM
                     IWAGRERVIILRPHALVALAEDFEPPPEAG"
     gene            2797..3249
                     /locus_tag="VFU72_09715"
     CDS             2797..3249
                     /locus_tag="VFU72_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423803.1"
                     /translation="MQTGNHRRRGGWTGQPRGRNATPAKTGTDGRHGSWTEVRALGRR
                     ARFWLTWRLALVVDRTGIGGAARLDGAREQRADQGTKIMPGITPPELANLLDAGLEPH
                     QISYLCFMRWRFRRGELNEGKLVSKALTMAAARATSGAVRAPAGRLTV"
     gene            3354..>4327
                     /locus_tag="VFU72_09720"
     CDS             3354..>4327
                     /locus_tag="VFU72_09720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5423804.1"
                     /translation="MRQILYLGTYERDYPRNALTIAGLRRAGYRVRELHAPVWELQSD
                     KSGGWLRLGPLLRLAARLPLVYLRLLARLPRALRGVDLVVIGYIGQSDMLLFGPLLRL
                     LGRPILFNPLLTLTDTLVEDRALMHPGSYPARLLRLIDRLSLALADAVLVDTRENGRF
                     LAEHFGVPPERIHWLPVGAAEHVFQPVEHRPTPEPEAPLRVLFYGKMIPLHGVETIVR
                     AAALLREEARFEIVGRGQMGEQARRLARRLGASNVNFTDWIPFERLPREIAGAHVVLG
                     IFGDSDKAARVVPNKVYQAMAMGAAIVTRDGPAMRQVLDDRSSALLVPP"
BASE COUNT          634 a         1511 c         1479 g          703 t
ORIGIN      
        1 gcgggcatcg acctcgttgg cgaccggcgc cgtcgctcac cggtgccccg tcggcctcca
       61 gcccgatcgc ggtgttgagc cggcgcagca gcatgccctc gtcgaccccg ccgatgcgcg
      121 ccgcctgcga caccgtcacc agcgagccct gtacccggcg cgcgatcggg ttctgcagcg
      181 cgcgaaaggc ggggttgatg gccgccagct catcgaccag ctcggggtgg cgcttgatga
      241 cctccgcgat cttccacgaa ccctgaatcg gttggagctc tgcctgcttg cgaagtcgga
      301 tcatggctta tccccaccaa ccgagttcat cgcgaccggc ctcggtgatc atctccggcg
      361 accaggccgg ttcccacacc agattgaccg cgacgtcctc gacggacggc acgaagacac
      421 gcaccgcctc gtcgacggcg gcggtcaggc tatcgtgcag cgggcagccg ggcgtggtca
      481 gcgtcatggt cacggtcacg ttcgagccgg tgatatcgac gccgtagacc agaccgagat
      541 cgacgacatt gatccccagc tcggggtcgt agacctcgta caaggctgcc cgcacggccg
      601 cctccgagag gaggagcgag ctcatcgggc acgctcctcc cgcgccggcg actcccccga
      661 ggccgccggc ctccccggaa ggtggaatgt acccgcctcg gagcggaagg ggatggccag
      721 gcgattccag ccgttgatcg cgatgatcgc catggtcagc ttgaccagtt cctcggcgct
      781 gaactgcccg cgcgcctgct cgtagacctc gtccggcacg tggtccaccg acaccagcgt
      841 gaccgcctcg gtccaggcca gcgcggcgcg ctcgcgctcg gagaagaagg gcgtctcgcg
      901 ccaggcgtcg agggtgaaga gccgctgggc ggactcgccg tgcgccatgg ccaccttgct
      961 gtgcatgtcg aggcagtagg cgcagccgtt gatctgcgag gtgcgcatgc gcaccaactc
     1021 cagcagcgcc ggctccagcc cgctatggcg cacgtagtgc tcaagggtct ccagaccctc
     1081 agtggctccc ggcgcgacct tgtggtagtg gatccgtggt tccattgctc atcccctcta
     1141 accgcgctcg gccgcggcct tggcgaagta ctgcttattc accaggagaa agttggtcca
     1201 ctcctcgggg aggtcgtcct cgaagaagat cgcctcgacc gggcagacct cggcacagac
     1261 gccgcagtcg atgcactcgt cggggtcgat gtagtactgc tcggccgcgt cgtcggtgtg
     1321 gatgcaatcg accgggcaga cctcgacgca ggagccgtcc ttgacgccga tgcagggctg
     1381 ggcgattacg taggtcatct cgatccgatc ctctctgctt caggcggact ccgccgtgtc
     1441 cggctgctcg gcagccagcg cgcgaatctc ggccagcatg ctgtcgagcg gctggcggta
     1501 gacgaggcag acgtcgcgca gcgactcgaa gcgcgccagc gggcacccga cgcagtgcat
     1561 gcgccgccgg atgaaggcgt tcgcggcccg cgggtgttgc tcgatgatct cgtcgatgga
     1621 gctttcctgc gtaacctgca tactgccatc cactcgtacg cggcgcggtg ccgctctcgg
     1681 gttctcgcgc cagcatggca cgccggcccg cgcccgtgtt tgcgctagcg caaagagcgg
     1741 gtggttgggc gtcgtggcga ttcccaggtt gggacgttgg ggtggagcgg cacctcgcgc
     1801 gctcgacggg cagcggcgcg ggcttcagcc ggcctcgggc ggcggctcga agtcctcggc
     1861 cagggccacc agggcgtgcg gccgcagaat gatgacgcgc tcgcggcccg cccagatcat
     1921 cccctgctgc tgccaggcgc tgagcgtgcg gctggccgtg ctcagcgtcg tcccggccag
     1981 ttctgccagg tcctggcgcg agagcggtag gtcgatctcg atgccgcgct ccgtcttgcg
     2041 gccgagcttg tccgtcagcc gcagcagagc gcgggcgatg cggcgctcga cgcgctcggt
     2101 ctgcaactcc tggatgcgcg cctcggcctc gcgcagccgc gcggccagct cggcgaccag
     2161 tgccagcgcg aactccggat gctttttcgc cagcgtggtg agctcgcgcg ccggcagccg
     2221 cagcacgacc gcgtcctcga gcgcgatcgc gctggccggg tagctatcag cgccccagat
     2281 gccggcgccg ccgaagatct caccgggctg gatcaggcgc aggatgacct cgcggccgtc
     2341 gtcggtctcg tggatgacct tgatgcggcc cgaggccagc aggttgatcg cgtcggcccg
     2401 ggccccttcg acgaagacga agtcgccatc ggcgaccggc gtcacgcgca tacgcccggc
     2461 gatcgcccgc agcgtttcgg gcagcagatc acgaaagaga cgcaggctgg cgaggaaggc
     2521 cgcccgctcg gggatcggcc aggccgcctg gttgtcgtcg tgagtggtca tcgtgctgtc
     2581 gccgccggcc catccttcgg atcgtgcgga gcgccgcgcc ccgctcctag tctacccgca
     2641 tcaccgggag cgcgaagagg actacggcac agaccctgcg ttagccctct tgagcaacgg
     2701 cggatggggc ggctcaccgc cccgccgaac ctcaccggac cgcgatccca ggtggttggt
     2761 gtgcgagcgt cgtaccgtcg agttcggagt ggggtggtgc agacaggcaa ccatcgtcgg
     2821 cgtggtggct ggaccggaca gccgcgcggc cgtaacgcga cgcccgccaa gaccggtacc
     2881 gacggccgcc acggctcgtg gaccgaggtg cgggcgctcg gccggcgcgc ccgcttctgg
     2941 ttgacctgga gactcgctct cgtggtcgac cgcacgggga tcggcggcgc cgctcgattg
     3001 gatggagcgc gcgagcagcg cgcggatcaa gggacgaaga tcatgccagg aataactcct
     3061 cctgaactgg ccaatctcct cgacgcgggg ctggagcccc atcagatcag ttatctctgt
     3121 ttcatgcgct ggcgcttccg ccgcggcgag ctgaatgagg gcaagctcgt ctcgaaagcg
     3181 ctcacgatgg cggccgctcg cgcgaccagc ggcgccgttc gcgccccagc aggccgcctc
     3241 acggtctgaa ggccggcctc tctcgatcct ccctgccgga ccgcccgccg tcgcgccgta
     3301 taatcgctcc gtgcgtaacg cggagttgag ggccactggc ggaacggcga gcggtgaggc
     3361 agatcctcta tctcggaacc tatgagcgtg actatccgcg caatgccctg acgatcgccg
     3421 gactccgccg cgccggctac cgggtgcgcg agctgcacgc gcccgtctgg gagcttcagt
     3481 ccgacaagag cgggggctgg ctgcgcctcg gccctctgct gcgactggcg gcccggctgc
     3541 cgctcgtcta cctcaggtta ctcgcgcgct tgccgcgggc gctgcgcggc gtcgacctgg
     3601 tggtcatcgg ctacatcggg cagtccgaca tgctcctgtt cggcccgctg ctgcggctgc
     3661 ttgggcgccc catcctcttc aacccgctgc tgacgctcac cgataccctg gtcgaagacc
     3721 gcgcgctgat gcaccctggc tcgtaccccg cccggctgct gcgactcatc gaccgcctct
     3781 cgctggcgct ggccgatgcc gtgctggtcg acacccgcga gaacggccgc ttcctggccg
     3841 agcatttcgg agtgccgccg gagcggattc actggctgcc ggtcggggcc gctgagcatg
     3901 tgtttcaacc cgtagaacat cgacccacgc cggaaccgga ggcgccgctg cgcgtcctct
     3961 tctacggcaa gatgatcccg ctgcacggcg tcgagacgat cgtgcgggcc gcggccctgc
     4021 tacgcgaaga agcccgcttc gagatcgtcg gccgcgggca gatgggcgag caggcacgaa
     4081 gactcgcccg ccggctcggg gcgagcaacg tgaacttcac cgactggatt cccttcgagc
     4141 gacttcccag agagattgcc ggcgcgcatg tcgtgctcgg catcttcggc gatagcgaca
     4201 aggcggcgcg ggtagtgccc aataaggtct accaggccat ggcgatgggc gcggcgatcg
     4261 tgacgcgcga tgggccggct atgcgtcagg tgctcgacga tcgctcctcg gccctgctgg
     4321 tgccgcc
//
LOCUS       DASUBI010000504         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_291359, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000504 DASUBI010000000
VERSION     DASUBI010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291359"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFU72_09725"
     CDS             <1..228
                     /locus_tag="VFU72_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5423805.1"
                     /translation="ATSIPVQLHVAEPDVYETEDWLNAWYLRMLRAGADVEIHRYRGA
                     GHLYTDPDLPDYDAEAAERTWATAQDFLAGL"
     gene            358..>2250
                     /gene="dnaE"
                     /locus_tag="VFU72_09730"
     CDS             358..>2250
                     /gene="dnaE"
                     /locus_tag="VFU72_09730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5423806.1"
                     /translation="MTDQPFTHLHVHTQYSLLDGAARLKDMFKACNEMGMSHVAITDH
                     GNLHGAYDFFHQAKAAGVTPVMGIEAYLAPESRRYTKPVRWGAPHQKNDDVSGAGAYT
                     HMTIWARDRTGLHNLFRAQSRASFEGYFRKPRMDRELLSEYAEGLMGTTGCPSGEVST
                     RIRLGQMDEALKAASEYQDIFGKENFFVEIMDHGIDIDRRARDGLMEISRKLKAPFVV
                     TNDSHYTYAQESAAHDTLLCIQTGSNLSDPDRFKFDGSGYYLKSAAEMYAIDSSDAWQ
                     EGCRNTQRLIADRIDTEGMFQFRNLMPRFDVPEGYDEVSWFREEVARGMARRYPGGIP
                     EDRQKLAEYEMDTIITMGFPGYFLVVADFIMWAKNNGIAVGPGRGSAAGSIVAYAMGI
                     TDLDPVPHGLIFERFLNPERVSMPDVDIDFDERRRGEVIRYVTEKYGSDKVAQIATYG
                     TIKAKAAIKDSARVLGYPFSMGDRITKAMPADVLGKGIPLSGITDKDHPRYSEAAEVR
                     AMYENEPDVTKVIDSARGIEGLVRQMGVHAAGVIMSAEPLIDHVPIFSPKNDGAVVTQ
                     WDYPSCESLGLLKMDFLGLRNLTIMDDAVKMVKSNKGIDIDLLSLPLDDPTTFELLQR
                     GDTLGVF"
BASE COUNT          397 a          825 c          711 g          317 t
ORIGIN      
        1 gccacctcga tcccggtcca gctgcacgtg gccgagccgg acgtgtacga gaccgaggac
       61 tggctgaacg cctggtacct gcgcatgctg cgcgcgggcg cggacgtcga gatccaccgc
      121 taccgcggcg ccggccacct ctacacggat cctgacctgc ccgactacga cgccgaggcc
      181 gcggagcgca cctgggcgac cgcgcaggac ttcctcgccg gactgtgagc ccggccgccc
      241 ccggaacgca ggccccggcc ggccttcacg gggagccgcg cccggctgtc gtacccgggt
      301 cgtagactcg ccagcgccgc caacaaaaga ccgtgctcgc cggaggagtc gccgcccgtg
      361 accgaccagc ccttcactca tctgcacgtc cacacccagt actcgctgct ggacggggcg
      421 gcgcggctga aggacatgtt caaggcgtgc aacgagatgg ggatgtccca cgtggccatc
      481 accgatcacg gcaacctgca cggcgcttac gacttcttcc accaggccaa ggcggccggc
      541 gtcaccccgg tgatgggcat cgaggcgtac ctggcgcccg agtcccggcg gtacaccaag
      601 ccggtccgct ggggcgcccc gcaccagaag aacgacgacg tgtccggcgc cggcgcctac
      661 acccacatga ccatctgggc ccgtgacaga accggcctgc acaacctctt ccgcgcccag
      721 tcccgcgcct ccttcgaggg gtacttccgc aagccccgga tggaccgcga gctgctcagc
      781 gagtacgccg agggcctgat gggcacgacc ggctgcccgt cgggcgaggt gtccaccagg
      841 atccggctcg ggcagatgga cgaggcgctg aaggccgcct ccgagtacca ggacatcttc
      901 ggcaaggaga acttcttcgt cgagatcatg gaccacggca tcgacatcga ccgccgggcc
      961 cgcgacggac tgatggagat ctcccgcaag ctcaaggcgc cgttcgtcgt caccaacgac
     1021 tcgcactaca cgtacgccca ggagtccgcc gcgcacgaca ccctgttgtg catccagacc
     1081 ggctccaacc tctccgaccc cgaccggttc aagttcgacg gctccggcta ctacctgaag
     1141 tcggccgccg agatgtacgc gatcgactcc tccgacgcct ggcaggaggg ctgccgcaac
     1201 acccagcggc tgatcgccga ccggatcgac accgagggca tgttccagtt caggaacctc
     1261 atgccgcgct tcgacgtgcc cgaggggtac gacgaggtct cctggttccg cgaggaggtc
     1321 gcccgcggca tggcccgccg gtacccgggc ggcatccccg aggaccgcca gaagctcgcc
     1381 gagtacgaga tggacaccat catcacgatg gggttccccg gctacttcct ggtggtcgcc
     1441 gacttcatca tgtgggcgaa gaacaacggc atcgcggtcg gccccggccg cggctccgcg
     1501 gccggctcga tcgtcgcgta cgcgatgggc atcaccgacc tcgacccggt gccgcacggg
     1561 ctgatcttcg agcggttcct caaccccgag cgcgtctcca tgcccgacgt cgacatcgac
     1621 ttcgacgagc gccggcgcgg cgaggtcatc cggtacgtca cggagaagta cggctccgac
     1681 aaggtcgcgc agatcgccac ctacggcacc atcaaggcca aggccgcgat caaggactcg
     1741 gcccgggtgc tgggctaccc gttctccatg ggcgaccgga tcaccaaggc gatgcccgcc
     1801 gacgtcctcg gcaagggcat cccgctgtcc ggcatcaccg acaaggacca cccgcgctac
     1861 agcgaggccg ccgaggtccg cgcgatgtac gagaacgagc cggacgtcac caaggtcatc
     1921 gactccgccc gcggcatcga gggcctggtc cggcagatgg gcgtgcacgc ggccggcgtg
     1981 atcatgtccg ccgagccgct gatcgaccac gtgccgatct tctcgccgaa gaacgacggc
     2041 gcggtcgtca cgcagtggga ctacccgagc tgcgagtcgc tcggcctgct gaagatggac
     2101 ttcctgggcc tgcggaacct gacgatcatg gacgacgccg tcaagatggt gaagtccaac
     2161 aagggcatcg acatcgatct gctgtcgctg ccgctggacg accccacgac cttcgaactc
     2221 ctccagcgcg gcgacaccct cggcgtcttc
//
LOCUS       DASUBI010000505         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_726030, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000505 DASUBI010000000
VERSION     DASUBI010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726030"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFU72_09735"
     CDS             <1..1586
                     /locus_tag="VFU72_09735"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HEU5423807.1"
                     /translation="LTMSLEILAEAAAALLPGRRVIGLRDVRAFRWLACDQPRQTLEV
                     SATRQGGSADEDRVVVRLRNLTEDAASNNPQTSPVLEATVILAGDYPGAPAPRSARLT
                     DGTPYSLPAGQLYREIMFHGPSWQGVTGMRETAPDGALAELCVLPWDGLLRGNPAPSF
                     VLDPVVLDAAGQVIGFWTAERLATGKIIFPFHLESLEVYGPQRPSGERLICAATIQLQ
                     GEHLVRSDIDVIDAEGALWLRLVGWEDKRFAVPRQFEPLVKGTQRAPISAEWPEPLAR
                     LAGRERLACRRLTANFPSDRAFWMGIWANRVLSRREREVFAQLHRPEPRRLEWLAGRT
                     AAKEAVEQLVKAHYRLELLPADIEILADSNGRPLVAGSWLERLPYPPLVSISHSGGTA
                     AAIACLSQDEASTALGIDIECTIPAREEFDAVAFTEDEERLLAPVPAVERAVWSLRAW
                     CAKEATAKALGEVMIEGPRTVLLAAVEPAAGSIAVRLGQQLATRHPALADLPLVVATG
                     QQDGIIYAVTRCEPATTRR"
     gene            1636..1944
                     /locus_tag="VFU72_09740"
     CDS             1636..1944
                     /locus_tag="VFU72_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEU5423808.1"
                     /translation="MSGAIDQQVLASVLELMTKLAGDWEYDDEITPDTYLLGDLGLES
                     LDLVVLGTSIQQRYGRLPFAEYLAEIGQRPVEERDITVGDLVGFVCRHRVPAVTGGGQ
                     "
     gene            1941..>3150
                     /locus_tag="VFU72_09745"
     CDS             1941..>3150
                     /locus_tag="VFU72_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5423809.1"
                     /translation="MSTSDKVLFLGLDGATFTVLDPLMRRGVMPFLRDFVQGGVRGRL
                     RSILPPLTPPAWTSLMTGKRPGEHGVFDFLQKEEPDSRYFRMAASQNIGAATVWSMVS
                     EQGGRVTALNFPLMFPPPAVNGSVVPGGWMPWRQLRLGCYPPGLFDRLKQLPGFNARE
                     MALDMAQEAKAIEGCDEGEYVDWITLHTRREQRWFEIMRYLMSEEPAQLSGIVFDGVD
                     KLQHQCWRFLDPALRPAEPTAWEQEVMGLCDEYFRQLDAILAALVEQAGPGATVIMGS
                     DHGFGPTSDIAHLNSWLEREGYLAWSEESAPRSDEADLQLGFAQMTRHVYQLDWERTR
                     AYVATPSSQGVHIVRERPDGSRLSDAEYQQLRDELVAKLRALINPATGKPLAAAVQTR
                     EEVYAGPFAAL"
BASE COUNT          464 a         1119 c         1113 g          454 t
ORIGIN      
        1 cgctgaccat gagcctggag atcctggccg aggccgccgc cgcgctgctg ccgggccgcc
       61 gggtgatcgg gctgcgcgac gtgcgcgcct tccgctggct ggcctgcgac cagccgcgcc
      121 agacgctcga agttagcgcc acgcgccagg gtggcagcgc cgacgaagat cgggtcgtgg
      181 tgcgcctgcg caacctgacc gaggacgccg ccagcaacaa cccccagacc agtccggtgc
      241 tggaagccac cgtcatcctg gccggcgact accccggcgc tccggcgccg cgttccgcac
      301 ggctgacgga cggcacaccg tacagcctgc ccgccggcca gctctaccgc gagatcatgt
      361 tccacggccc atcctggcag ggcgtcacgg gcatgcgcga gaccgcgccc gacggggcgc
      421 tggccgagtt gtgcgtcctc ccctgggatg ggctgctgcg cggcaacccc gcgccgtcct
      481 tcgtgctcga cccggtggtg ctcgacgctg ccggccaggt catcggcttc tggaccgccg
      541 agcgtctggc cacgggcaag atcatcttcc ccttccacct ggagtcgctg gaggtctacg
      601 gcccgcagcg gcccagcggc gagcgcctga tctgcgccgc gacgattcag ctgcagggcg
      661 agcatctggt gcgctccgac atcgacgtga tcgacgccga gggcgcgctc tggctgcggc
      721 tggtgggctg ggaggacaag cgcttcgccg tgccccggca gttcgagccg ctggtcaagg
      781 ggacccaacg cgcgccgatc tcggccgagt ggcccgagcc gctggcacgc ctcgccgggc
      841 gcgagcggct ggcctgtcgc cgcctgacgg ccaacttccc ctccgaccgt gccttctgga
      901 tgggtatctg ggccaaccgc gtgctgagcc ggcgcgagcg cgaggtgttc gcccaactgc
      961 accggccgga gccgcgccgc ctggagtggc tggctgggcg cacggccgcc aaagaggcag
     1021 tggagcagct cgtcaaggcg cactataggc tggaattact gccggccgac atcgagatcc
     1081 tggccgacag caacggtcgg ccgctggtcg ccgggagctg gctggagcgg ctaccgtacc
     1141 cgccgctggt ctcgatcagc cacagcggcg gcaccgccgc cgccatcgcc tgtctgtcac
     1201 aggacgaggc gtcgaccgcc ctcgggattg acatagagtg tacaatccct gcgcgcgagg
     1261 aattcgacgc ggtggccttt accgaggatg aagagcggct gctggcgcct gtgccggcgg
     1321 tggaacgcgc cgtctggtcg ttgcgcgcct ggtgcgccaa agaggccacg gccaaggcgc
     1381 tgggtgaggt catgatcgag ggaccgcgca cggtcctcct ggcggccgtc gagccggcgg
     1441 cggggtcgat cgcggtgagg ctcggtcagc aactggcgac gcgccacccc gcgctggcgg
     1501 acctgccgct cgtcgtcgcg accgggcagc aagacgggat catctacgcc gtgacgcgct
     1561 gcgaaccggc gacgacgcgg cgctagctcg cgccggacga caccggcagt ggaacggaga
     1621 gggaaaggga catcggtgag cggagcaatc gatcaacagg tgctggccag cgtgctcgaa
     1681 ctcatgacca agcttgccgg tgactgggaa tacgacgacg agatcacgcc ggacacctac
     1741 ctgctgggcg atttgggcct agagtcgttg gatctggtcg tgctgggcac ctcgattcag
     1801 cagcgctacg ggcgcctccc cttcgcggag tatctggccg agatcggcca gcggccggtc
     1861 gaggagcgcg acatcaccgt cggcgacctg gtgggcttcg tctgccggca ccgcgtgccc
     1921 gcggtgacgg ggggcgggca atgagcacga gcgacaaggt cctcttcctc ggactcgacg
     1981 gcgccacctt caccgtcctc gacccgctga tgcggcgcgg cgtgatgccc ttcctgcgcg
     2041 acttcgtgca gggcggcgtg cgcgggcgcc tgcgcagcat cctgccgccg ctgaccccgc
     2101 cggcctggac cagcctgatg accggcaagc ggccgggcga gcacggcgtc ttcgacttcc
     2161 tgcagaagga ggagcccgac agccgctact tccgcatggc cgcctcgcag aacatcggcg
     2221 cggcgacggt ctggtcgatg gtctccgagc agggcgggcg cgtcaccgcg ctgaacttcc
     2281 cgctgatgtt cccgccgccg gcggtcaacg gcagcgtcgt gccgggcggc tggatgccct
     2341 ggcgccagtt gcgcctcggc tgctacccgc cgggcctctt cgaccgcctc aagcagctcc
     2401 ccggcttcaa cgcccgcgag atggcgctcg acatggcgca ggaggccaag gcgatcgagg
     2461 gctgcgacga gggcgagtat gtcgactgga tcacgctcca cacccgtcgc gagcagcgct
     2521 ggttcgagat catgcgctac ctgatgagcg aggagccggc gcagctctcc ggcatcgtct
     2581 tcgacggcgt ggacaagctg cagcaccagt gctggcgctt cctcgacccc gccctgcgtc
     2641 cggccgagcc gactgcctgg gagcaggagg tcatggggct gtgcgacgag tacttccgcc
     2701 agctcgacgc catcctggca gccctggtcg agcaggccgg ccccggggcg accgtgatca
     2761 tgggctccga ccacggcttc ggccccacca gcgacatcgc ccacctcaac tcctggctgg
     2821 agcgggaggg ctacctggcc tggtccgagg agtccgcacc gcgctcggat gaagccgacc
     2881 tgcagctcgg cttcgcccag atgacccggc acgtctacca gctcgattgg gagcgcaccc
     2941 gcgcctacgt cgccaccccg agcagccagg gcgtgcacat cgtgcgcgag cggccggatg
     3001 gctcgcggct ctcggacgcc gaataccagc agttgcgtga cgagctggtg gcgaagctgc
     3061 gcgcgctgat caacccggcc accggcaagc cgctcgccgc ggccgtgcag acgcgcgaag
     3121 aggtctacgc cgggccgttc gccgcgctgg
//
LOCUS       DASUBI010000506         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1351009, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000506 DASUBI010000000
VERSION     DASUBI010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351009"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /locus_tag="VFU72_09750"
     CDS             <1..2427
                     /locus_tag="VFU72_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5423810.1"
                     /translation="MRTDGRTKGHGKVFANPAEAVLAYHSGVVDLQAPVMVRMSLDGS
                     KPQLVETTPGRIIFNEVIGRYNDQVGPEQQIAYRNVALDKGALRGVIGECHRKLGAAR
                     TAEIADNMKRLGFTYATKSGMSVAVADVEIPAAKDQMLADADAEVDQIEREYRRGLLT
                     DNERYQQVIEVWNRTTDEMTDELKKILDPYGLVNTLASSGATKARYKQIRQLAAMRGL
                     MADPSGRIIELPIRSNFREGLTVLEYFVSTHGGRKGLADTALRTADAGYLTRRLVDVA
                     QDVLITMEDCGTTAVSTIVEPPNTDVAESYAARVVGRFTAQPVVDPKTGEIIVDANEE
                     VTDVIMQRIEEAGIKSLPMRTPLTCDAQHGICQLCYGRSLATGEIIAIGEAVGIIAAQ
                     SIGEPGTQLTLRTFHTGGVASSEDITQGLPRVEELFEARVPKGQAILAEIDGKIELTR
                     EDEIRRIKIVSSEIFNDPYQLPEGGHYELLVRPEQDVAENTPLFRSTLAGEEDNVTVA
                     HTAGKVLIDEENNQLILRSEERDEREYQVLAGSRLTVEDGDYVTAGTPLTEGSQNPQE
                     ILAIMGREAVQRYLVDQVQTVYRLQGVNVHDKHIEAIVRQMLMKVRIDLPGDTDLLPG
                     ELIDRFKFEETNARVLAEGGEPATATPVLLGITKASLSTDSFLSAASFQETTRVLTEA
                     AINGQIDRLRGLKENVIIGKLIPAGSGATRRPQLGDLTMVDEGTNIDDYLRISQGGDE
                     EIPADATGSGGEDEDSVVSLETAGADPEFALSELLGQSVLVGGDSDSDDPTEAEAILI
                     DQDNLDEPAD"
BASE COUNT          492 a          837 c          844 g          371 t
ORIGIN      
        1 atgcgcaccg acggccgcac caagggccac ggcaaggtct tcgccaaccc ggcggaggcg
       61 gtgctggcct atcacagcgg cgtggtcgat ctgcaggcgc cggtcatggt gcgcatgtcg
      121 ctcgacggta gcaagccgca gttggtcgag acgacgccgg gccgcatcat cttcaacgag
      181 gtgatcgggc gctacaacga tcaggttggc ccggagcagc agatcgccta ccgcaatgtc
      241 gcgctcgata agggcgcgct gcgcggtgtg atcggcgagt gccaccgcaa gttgggcgct
      301 gcccgcacgg cggaaatcgc cgacaacatg aagcgcctgg gcttcaccta cgccaccaag
      361 tccggcatgt ccgtggccgt ggccgatgtg gagatcccgg cggccaagga ccagatgctg
      421 gccgacgccg atgccgaagt cgatcagatc gagcgcgagt atcgccgcgg tctgctcacc
      481 gacaacgagc gctaccagca ggtcatcgag gtctggaacc ggaccactga tgagatgacc
      541 gacgaactca agaagatcct ggacccgtac ggcctggtga acacgctggc tagctcgggc
      601 gcgacgaagg cgcgttacaa gcagatccgc cagttggccg ccatgcgcgg cctcatggcc
      661 gacccgtccg ggcgcatcat cgagctgccc attcgctcga acttccgcga agggctgacg
      721 gtgctggagt acttcgtgtc cacccacggt gggcgcaagg gtctggccga taccgcgctg
      781 cgcacggcgg acgccgggta tctcacccgc cgcctggtcg atgtggcgca ggacgtcctg
      841 atcacgatgg aggactgcgg caccaccgca gtgtcgacca tcgtcgaacc gccaaacacc
      901 gatgtggccg agagctacgc cgctcgcgtg gtgggccgct tcacggcgca gccggtggtc
      961 gatcccaaga cgggtgaaat catcgtcgat gccaacgagg aagtcaccga tgtgattatg
     1021 cagcggatcg aggaggcggg catcaagtcc ctgccgatgc gcacgccgct gacgtgcgat
     1081 gcccagcacg gcatctgcca gctttgctac ggtcgctcgc tggcgaccgg cgagatcatc
     1141 gccatcggcg aggcggtcgg catcatcgcc gcccagtcca tcggcgagcc aggtacgcag
     1201 cttacgttgc gcacgttcca caccggtggt gtggcttcgt ccgaagacat cacccagggt
     1261 ctcccgcgcg tcgaggagct cttcgaggcg cgcgtgccca agggccaggc catcctggcg
     1321 gaaatcgacg gcaagatcga gctgacgcga gaggacgaga tccgccgcat caagatcgtg
     1381 tcgagcgaga tcttcaacga tccgtaccag ttgccggagg gcgggcacta tgaactgctc
     1441 gtccgccccg agcaggacgt ggcggagaat acgccgctct tccgctcgac cctggcgggc
     1501 gaggaggaca acgtcaccgt cgcccacacg gcgggcaagg tgctgatcga tgaggagaac
     1561 aaccagctta tcctgcgcag cgaggagcgc gacgagcgcg aataccaggt gctggccggg
     1621 tcccggctca cggtggaaga cggcgactat gtcaccgccg gcacgccgct gaccgaaggc
     1681 tcgcagaacc cgcaggaaat cctggcgatc atgggccgcg aggccgtgca gcgctacctg
     1741 gtggaccagg tgcagacagt gtaccgcctg cagggtgtga atgtccatga caagcacatc
     1801 gaggccatcg tgcggcagat gctcatgaaa gtgcgcatcg acctgccggg cgataccgac
     1861 ctgctgccgg gcgagttgat cgatcgcttc aagttcgagg agaccaacgc ccgcgtgctg
     1921 gccgaaggcg gcgagccggc gacggccacg cccgtcctgc tcggcatcac caaggccagc
     1981 ctgagcaccg acagcttcct gtcggcggcc tccttccagg agaccacgcg cgtgctgacc
     2041 gaggcagcca tcaacggcca gatcgaccgg ctgcgcggcc tgaaggagaa tgttatcatc
     2101 ggcaagctga tccccgccgg cagcggggcc acccgccgcc cgcaactcgg cgacctgacc
     2161 atggtggacg agggaaccaa catcgacgac tacctgcgca tcagccaggg cggcgacgag
     2221 gagatccccg ccgacgccac cgggtcgggc ggcgaggacg aggacagcgt ggtttccctg
     2281 gaaaccgccg gggccgatcc ggagttcgcg ctcagcgaac tcctcggcca gtcggtcctc
     2341 gtcggcggcg acagcgacag cgacgacccg acggaagccg aggccatcct gatcgatcag
     2401 gacaacctcg acgagcccgc cgactaatcg cgaatggcct gctaagcaag agagccgccg
     2461 gtgttctgcc ggcggctctt ttcgccaatg tatagccggt tgtccggcac gggccggttt
     2521 caaaccggcc cctacaccaa atag
//
LOCUS       DASUBI010000507         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_408265, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000507 DASUBI010000000
VERSION     DASUBI010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408265"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFU72_09755"
     CDS             <1..985
                     /locus_tag="VFU72_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEU5423811.1"
                     /translation="VALVLGDPVGPPEGAARAIDEFLDICAVNGWTPAFYGASARYLP
                     QFARHGLAAAQVGEDTVIDLAALSFSGKGWQNVRTALNRAAREGIAFAFLDPRKAEPA
                     IVEQLFAIDRDWVAARNLPEMGFTLGKLTLPLDPEVRLAVALDAEGRALAYVSWLPVY
                     ARHGWVLDLMRRRADAPHGVMEFLIAKSALAFKSEGAASLSLAVAPLARVARPEGETR
                     LFERVLVTLGERLDSFYHFSSLCDFKRKFKPTWLPVYLVYPGAASLPRIGYAVLRAYL
                     PTLSAHDVGTLLSRAAPLPRPHRSTPPPAPATPCDPGQTSDSETLVGAGRR"
     gene            1072..2712
                     /locus_tag="VFU72_09760"
     CDS             1072..2712
                     /locus_tag="VFU72_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5423812.1"
                     /translation="MTGGQALAGQLAREGIDTVFGLPGIQLDWAFDALYQARDQIRVV
                     HTRHEQATTYMADGYARTTGKVGTAIVVPGPGLLNAMAGLATAYACNAPVLCLAGQID
                     SAWIDRACGVLHEIPHQLRAARTVSKWAGRAGRPEEIPGRVHTAVRRALSGRPRPVVV
                     ELPPDVLRDRAPIRLRRAEPVIPPAPDPDAVARAAKILGRAKQPLILAGGGANRPEAA
                     APLRALAEQLEAPVVLTINGKGALSARDYHAQTMLAARELLPAADAILAVGTRLVEPA
                     TESWGLNGQPLIQLDIDPAELGRNHRATVALAADAALGLGALLDALGRHNQRRASRQE
                     ELSALQERIDDRLFEVQPTASYALAIREELPDEAIVVVDSTQVGYWSWAGFPVYQPRT
                     YLTAGYQGTLGFAYASALGAQAGNPDTPVVSISGDGGFLYTIQELATATRHGLNVVAI
                     VFSDDAYGNVRMIQRRQFGGHVIATELDNPDFVKLAESFGVEGRRATNPEGLRAALRE
                     SLAAKRPALIEVPTGELASVFPLLMAGRRKPTWCWAGE"
     gene            complement(2716..>3131)
                     /locus_tag="VFU72_09765"
     CDS             complement(2716..>3131)
                     /locus_tag="VFU72_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5423813.1"
                     /translation="ASDAASFSTAFSRIGLAPDSGMSYFLPRLVGWGRAFELLARSPR
                     LSAEEAAALGIVSQVSPAAEFAGTVRAVAAEFAAGPTKAYGLIKRALRQSAGASFSEA
                     LGFEAHLQYIASQSADFQEGVAAFLEKRRPAFQGQ"
BASE COUNT          419 a         1188 c         1109 g          415 t
ORIGIN      
        1 ggtggcgctg gtgctgggcg atccggtcgg cccgccggag ggcgcggcgc gcgccatcga
       61 cgagttcctc gacatctgcg cggtcaacgg ctggacgccg gccttctacg gcgccagcgc
      121 gcgctacctg ccccagttcg cccggcatgg gctggccgcg gcccaggtcg gcgaggacac
      181 cgtcatcgac ctggccgcgc tcagcttcag cggcaagggc tggcagaacg tgcgtaccgc
      241 gctcaaccgc gccgcgcgcg aggggatcgc ctttgccttc ctcgacccgc gcaaggccga
      301 gccggccatc gtcgagcagc tcttcgccat cgaccgggac tgggtggcgg ccaggaacct
      361 gcccgagatg ggcttcaccc tgggcaagct gacgctcccg ctcgaccccg aggtgcgcct
      421 ggcggtggcg ctcgacgccg aggggcgggc gctggcctac gtctcctggc tgccggtcta
      481 cgcccggcac ggctgggtgc tcgacctgat gcggcggcgc gccgacgccc cgcacggcgt
      541 gatggagttc ctgatcgcga aatcggcgct ggccttcaag agcgagggcg cggcctcgct
      601 cagcctggcg gtggcgccgc tggcgcgcgt ggcgcggccg gagggtgaga cgcggctctt
      661 cgagcgcgtc ctggtcacgc tgggcgagcg gctggacagc ttctaccact tcagctcgct
      721 gtgcgacttc aagcgcaagt tcaagccgac ctggctgccg gtctacctgg tctaccccgg
      781 cgcggccagc ctgcccagga tcggctacgc cgtgctgcgc gcctatctcc ccacgctcag
      841 cgcgcacgac gtgggcacgc tcctctctcg cgccgcgccg ctgcctcgtc cacatcgctc
      901 cacgcccccg cccgcgcccg ctacgccgtg cgatcctggc cagacgtcgg acagcgagac
      961 gctggttggc gccgggcgcc ggtaggagtc tcgcgtattg gcataacgat tgataccgct
     1021 cctctcggga tgcaatggag caccccgaga ggagtggtcg gcgtgactga gatgaccggc
     1081 ggccaggccc tggccgggca gctcgcgcgg gaggggatcg acaccgtctt cggcctcccc
     1141 ggcatccagc tcgactgggc cttcgatgcc ctctaccagg cgcgcgacca gattcgcgtg
     1201 gtccacacgc gccacgagca ggcgaccacc tacatggccg acggctacgc ccgcaccacc
     1261 ggcaaggtcg gcaccgccat cgtcgtgccc ggccccggcc tgctgaatgc catggccggc
     1321 ctcgcgaccg cctacgcctg caacgcgccc gtcctctgcc tggcgggcca gatcgactcg
     1381 gcctggatcg accgcgcctg cggagtcctc cacgagatcc cgcaccagct ccgggccgcg
     1441 cgcacggtca gcaagtgggc cgggcgcgcc gggcggcccg aagagatccc cggccgcgtc
     1501 cacacggccg tgcgtcgcgc cctgagcggc cgaccacgtc cggtggtggt cgagctgccg
     1561 cccgacgtgc tgcgcgaccg cgctcccatc cggctgcgcc gtgccgagcc ggtgatcccg
     1621 cccgcgcccg atcccgacgc ggtggcgcgc gccgccaaga tcctggggcg ggccaagcag
     1681 ccgttgatcc tggcgggcgg cggcgccaac cggccggagg ccgccgcgcc gctgcgcgcg
     1741 ctggccgagc agctcgaagc gccggtggtg ctgacgatca acggcaaggg cgcgctctcg
     1801 gcgcgcgact accacgcgca gacgatgctg gcggcgcgcg agctgctgcc ggccgccgac
     1861 gcgatcctgg cggtcggcac gcgcctggtc gagcccgcga ccgagtcctg gggactgaac
     1921 ggccagccgc tgatccagct cgacatcgac ccggccgagc tcgggcgcaa ccaccgggcg
     1981 accgtcgcgc tggcggccga cgccgcgttg gggctcggcg cgctgctcga cgccctcggc
     2041 cggcacaacc agcggcgcgc ctcgcgccag gaggagctga gcgccctcca ggagcgcatc
     2101 gacgaccgcc tcttcgaagt ccagccgacc gcctcctatg ccctggcgat ccgcgaggag
     2161 ctgccggacg aggcgatcgt ggtggtcgac agcacccagg tcggctactg gagctgggcc
     2221 ggcttcccgg tctaccagcc gcgcacctac ctgaccgcgg gctaccaggg cacgctcggt
     2281 ttcgcctacg ccagcgcgct aggcgcgcaa gccggcaacc ccgacacgcc ggtcgtctcg
     2341 atcagcggcg acggcggctt cctctacacc atccaggagc tggccacggc tacccggcac
     2401 ggcctcaacg tggtggctat cgtcttcagc gacgacgcct acggcaacgt gcgcatgatc
     2461 cagcggcggc agttcggcgg gcacgtgatc gccaccgagc tggacaatcc cgacttcgtc
     2521 aagctggccg agtcgttcgg cgtcgagggc cgccgcgcca ccaaccccga ggggctgcgc
     2581 gccgccctgc gcgagagtct cgcggccaag cgcccggcgc tgatcgaggt gccgaccggc
     2641 gagctcgcca gcgtcttccc gctcctgatg gccggccggc gcaagccaac ctggtgctgg
     2701 gccggcgagt agcggctact gcccctggaa ggccgggcgg cgcttctcca ggaaggccgc
     2761 cacgccctct tgaaagtcgg cgctctggct ggcgatgtac tgcaggtgcg cctcgaagcc
     2821 cagcgcctcg ctgaacgacg cgcccgcgct ctggcgcagc gcgcgcttga tcagcccgta
     2881 ggctttggtg gggccggcgg cgaactcggc ggccaccgcg cgcacggttc cggcgaactc
     2941 cgccgccggg ctgacctggc tgacgatgcc gagggcggcg gcctcctcgg ccgagagccg
     3001 gggtgagcgc gccagcagct cgaaggcccg gccccagccg accagccggg gcaggaagta
     3061 actcatgccg gagtccggcg ccaggccgat gcggctgaag gcggtgctga aggaggccgc
     3121 gtccgaggcc a
//
LOCUS       DASUBI010000508         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_431338, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000508 DASUBI010000000
VERSION     DASUBI010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431338"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            922..2091
                     /locus_tag="VFU72_09770"
     CDS             922..2091
                     /locus_tag="VFU72_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5423814.1"
                     /translation="MQGLVSTPATAPSWSYVPDDAPAVERQALTGAVDLAARLAGGQR
                     ASLLLPDEPAGPLRVVAARGLATRLAGRVRVRLGEAVAGSVALSRRPLLVNERAVHPG
                     HQARGYQTGAFISVPVPLPDDACGVLNVADPLRPEGFQSDDLYALEQLACQITTGLGA
                     QQASRRNADLEQTVRQLRRQVVQVQEAERQRIARDLHDEAGHALTAAVLGLDLELMQL
                     GGDPAAGAALRRAREQLVACSSTLHTIAFNLRPRILEDFGLHAALRSLARHSMELTDI
                     EVTVAIEGPPWDLDEPHELAILRVVQEALNNARKYAQAGRVAIALRYEPRELTLEIAD
                     DGVGLSAQATAPLGHPSRAMLGIGGMRERIELLEGSFSIGQGAAGGTLIVARLPR"
     gene            2116..2850
                     /locus_tag="VFU72_09775"
     CDS             2116..2850
                     /locus_tag="VFU72_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423815.1"
                     /translation="MELQHVEAVGVGVGHEPAAAGESVEAIRLLLIEDHTIVREGLKL
                     LFAQLPGMRLIGDAANGEDGVRLFERLLRQGQLIDVVVSDLGLPDISGIEVTRRIKAA
                     RPETRVLILSMYADQEHIAGILESGVDGYLLKQSSPAELADAVQAVARGDMALSPAIA
                     RRLVTQMQRQLQREQLVQALTEREQQVLALLAQGGTSKQIARDLGLSIKTVENHRARI
                     LEKLGAANTAEAIGRAYQYGLIAERR"
     gene            3284..3988
                     /locus_tag="VFU72_09780"
     CDS             3284..3988
                     /locus_tag="VFU72_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5423816.1"
                     /translation="MTLRALVVSQHKTFRRDLARYVESLQPEVAVVGEVVGAYQALDR
                     VEVLQPELVLLDLALRPGSALELAGRIHCQSPATAVVVVGNEPAADYRDAALEAGALD
                     YVDVLELASQLPAALRLVERLSAYREEREAAPEPATEPQPQPGEATQRPPLARLRQRL
                     CTVPTGPKYGPYTAWQYIHVCLALVLAEALYILRRPDAPEQWQLVVLLCVAVVAVLEL
                     RQFSRARRLAVAVGCR"
     gene            4101..>4283
                     /locus_tag="VFU72_09785"
     CDS             4101..>4283
                     /locus_tag="VFU72_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423817.1"
                     /translation="MMLFNTLLQYAFDYAPAALKSEKGQDFAEYAIILALVVIIAAAG
                     FSGLGNAVLGVLQNVSG"
BASE COUNT          649 a         1487 c         1496 g          651 t
ORIGIN      
        1 gccgcacgag ctggcgccgg gtaagctgcc cggccacgaa ttgctgtcgt aggagtgaca
       61 agcgggtatc gttcacgtgc gttccttcct cctcaaaact cacgcttggg cgagatccga
      121 gaaaccgtct ctctggcggc aaagccgctc taccccgttt actccgccag gcgtcgatcc
      181 ttcctcgagt ccctggcccg gacccgacct tgctgcaacc gtgaaacgtg gcaccaccaa
      241 tacggccaac tgccgcgccc ggttcgtcgc tgcgcccgtg ttcgtccggg tattgcccgg
      301 catacacact ggatgcgcag agattacaca cttcgcgcgc ctaacgcaag ggagttgacg
      361 cgtcaggcgc gcggcgacgc gcgggagggg tggctggtcc gtcctccggc acggtactgg
      421 tagacgcggc ataggtacgt tcgtacctat gtcttgacgg cgtagtaccg atgtcctatc
      481 atgacatcag ggctgagcgc tactgttcct caatccgaag gcatgggcga tgtttgccgg
      541 tgtgtcacca aggtgcgggt gcggctggcc cgggccggcg ctgcccgcac gtgtcccaat
      601 cttgcccgtg tggcgctggt tcgtagcgga cgagatgact ggtgcgggat acgatagatc
      661 tagccctctt cgcgtatttg tacgtacacc gctaaaggcc aaactcacca caatcgcgag
      721 gaagacgctt gcaggttcgg gcacgatgcg ctatagtagg agcgggacat tcccgaaaat
      781 cgaaggattt atccccgcgg tggcgcatat ggcgccagcc ggctcgcccg cgcggggagt
      841 ctcgcccgtg tcgcggcgcg tcgcggccgc tgaactcggg tggcgccggg agcgcaggtc
      901 gtctgggaag ggatgacgtc gttgcagggg cttgtctcca cgcctgccac cgccccgagc
      961 tggtcgtatg tgccggatga cgcgccggcc gtcgagcgcc aggcgctgac cggcgccgtc
     1021 gatctcgccg cccgcctggc cggcggccag cgcgcctcgc tgctgctgcc ggacgagccg
     1081 gccgggccgc tgcgcgtggt cgccgcgcgc gggctggcga cgcggctcgc cgggcgggtg
     1141 cgcgtgcggc tgggtgaggc ggtcgccggc agcgtggccc tcagccgccg ccccctcttg
     1201 gtcaacgagc gcgcggtgca ccctggccac caggcgcgcg gttaccagac cggcgccttc
     1261 atcagcgtgc cggtgccgct gcccgacgac gcctgcggcg tgctcaacgt ggccgacccc
     1321 ctgcgccctg aaggtttcca atcggacgac ctgtacgctc tggagcagct cgcctgccag
     1381 atcaccaccg gcctgggcgc ccagcaggcc agccggcgca acgccgacct ggagcagacg
     1441 gtgcggcagc tgcgccgcca ggtggtgcag gtgcaggagg ccgagcgcca gcgcatcgcc
     1501 cgcgacctgc acgacgaggc cggccacgcg ctgaccgccg ccgtgctggg cctcgatctg
     1561 gagctgatgc agctcggcgg cgaccccgcg gccggcgcgg cgctgcggcg ggcgcgcgag
     1621 cagctcgtcg cctgcagcag cacgctgcac acgatcgcct tcaacctgcg cccgcgcatc
     1681 ctggaggact tcggcctgca cgccgccctg cgcagcctgg cgcgccacag catggagctg
     1741 accgacatcg aggtgacggt cgccatcgag ggcccgccct gggatctcga cgagccgcac
     1801 gagctggcca tcctgcgcgt ggtgcaggag gcgctgaaca acgcgcgcaa gtacgcccag
     1861 gccggtcgcg tcgccatcgc gctgcgctac gagccgcgcg agctgacgct ggagatcgcg
     1921 gacgacggcg tcggtctcag cgcgcaggcc accgcgccgc tcggccatcc gagccgggct
     1981 atgctcggca tcggcggcat gcgcgagcgc atcgagctgc tggagggcag cttcagcatc
     2041 gggcagggcg cggcgggcgg cacgctgatc gtcgcccggc tgccgcgctg agccggggag
     2101 acacggggga accgcatgga gctgcagcat gttgaggcgg tgggggtggg cgtgggccac
     2161 gagccggccg cggccgggga gagcgtggaa gccatccgcc tcctcctgat cgaggatcac
     2221 accatcgtgc gcgaggggct gaagctcctc ttcgcgcagc tgccgggcat gcggctcatc
     2281 ggcgacgccg ccaacggcga ggacggcgtg cgcctcttcg agcgcctgct gcgccagggg
     2341 cagctcatcg acgtggtcgt cagcgacctc ggcctgccag acatcagcgg catcgaagtc
     2401 acgcggcgca tcaaggcggc ccgccccgag acgcgcgtgc tgatcctctc aatgtacgcc
     2461 gaccaggagc acatcgccgg catcctcgag agcggcgtcg acggctacct gctcaagcag
     2521 tcgagcccgg ccgagctggc cgacgcggtg caggcggtgg cgcgcggcga catggcgctc
     2581 tcaccggcca tcgcgcggcg actggtgacg cagatgcagc ggcagctcca gcgcgagcaa
     2641 ctggtgcagg cgttgaccga gcgcgagcag caggtgctgg cgctgctggc gcagggcggc
     2701 accagcaagc agatcgcgcg cgatctcggc ctcagcatca agacggtcga gaaccaccgc
     2761 gcgcgcatcc tggagaagct cggcgcggcc aacaccgccg aggccatcgg ccgcgcctac
     2821 cagtacggcc tgatcgccga gcggcggtag ggccctcacc cccggcccct ctcccctgcg
     2881 ggggcgaggg gagctgccca tcgactccat tgattgccac accaacacgg atccttcggc
     2941 aagctcagga tgacagggaa gcggacgcgg cctaccgggc gacaccatac gtcgtcgtcc
     3001 gcctcatccc tcaccctgtt atcccgaccg tagtggaggg atccgtcctg ccttaccacc
     3061 caacccacat cactcgccac ctcccctctc ccgcgcacgg gagagggtta gggtgagggc
     3121 ctgtaccccc gcctccaaat tcgatttccc cctatacccc gctggggggt tttggccgca
     3181 ggcgctgagg agtgctgctc atgcggcaac cccaggcggc tcctatgctg gattcaccgc
     3241 tcaagggagc ggaacggcca cgtacggtgg gggagtcgag agcgtgacgc tgcgggcgct
     3301 ggtggtcagc cagcacaaga ctttccggcg cgacctcgcg cgctacgtgg agtcgctgca
     3361 gccggaggtg gcggtggttg gcgaggtggt cggggcctac caggcgctcg accgcgtgga
     3421 ggtgctgcag ccggagctgg tgctgctcga cctggccctg cggccgggga gcgcgctgga
     3481 gctggccggg cgcatccact gccagtcgcc cgcgaccgcc gtggtggtcg tcggcaacga
     3541 gccggcggcc gactaccgcg acgcggcgct ggaggcgggc gcgctcgact acgtcgacgt
     3601 gctcgagctg gcatcccagc tcccggccgc gctgcgcctg gtcgagcgcc tgtccgcgta
     3661 ccgcgaggag cgcgaggcgg cgccggagcc ggccaccgag ccccagccgc agcccggtga
     3721 ggccacgcag cgcccgccgc tggcccgcct gcgccaacgc ctctgcactg tgccgaccgg
     3781 gccgaagtac ggcccctaca ccgcctggca gtacatccat gtctgcctgg cgctggtgct
     3841 cgcggaggcg ctgtacatcc tacggcgccc ggatgctccg gagcagtggc agctcgtcgt
     3901 gctgctgtgc gtcgccgtcg tcgccgtgct ggagctgcgg cagttctcac gtgcccgccg
     3961 gcttgcagta gccgttggct gtcgctaacg agatcaacag tgggggaacg gagaggaggc
     4021 actatcgagg aagctgggag ccactcgcgc caatcggttc attgaacatc acgtagattg
     4081 tcagggagga cagaaacacc atgatgctct tcaacacgtt gctgcagtat gccttcgact
     4141 acgcacccgc cgcgctcaag agtgagaagg gccaggattt tgcggagtac gcgatcatcc
     4201 tcgcgctcgt ggtaatcatc gcggccgctg gcttctcagg tttgggcaat gccgtcctcg
     4261 gcgttctcca gaacgtgtcg ggc
//
LOCUS       DASUBI010000509         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1074475, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000509 DASUBI010000000
VERSION     DASUBI010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074475"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFU72_09790"
     CDS             complement(<1..215)
                     /locus_tag="VFU72_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5423818.1"
                     /translation="MTAEYQQIESDRPRARDLGVVLGTLPPGPLNAITDVPGVLVGQV
                     TLVAGQGALVPGVGPIRTGVTVILPHEG"
     gene            326..1555
                     /locus_tag="VFU72_09795"
     CDS             326..1555
                     /locus_tag="VFU72_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5423819.1"
                     /translation="MSARMVVFGAAWLVARAALTAEAAEMPEKGAGERERARRYNHAK
                     HVLLTLDLALSLVGGAALVLSGLPGDVRRRVFARASNRLAGRTAVIAVFTLGQFIVSL
                     PLGYYSGYLLEHRFDLSNQSRRGWLWEQVKGLLISLPLVAVLGNGLLWVIGRWPRRWW
                     LVVSALSLPFTVLFAQLGPVLIMPLFNKFEPLRDQQLAERLKALAARSGIHVAQVLEM
                     NMSKQTKKANAFFAGLGGTRRIVLGDTLVEDFSPEEIEVVVAHEIAHQAHHDIWRLIA
                     VGTVGTAASTFAVERIARWALERFPRRLHLHSLDDVAALPLLGIISGLVGLVLMPLAN
                     AYSRHLERRADGFALDLTRTPRAFIGSMRQLAEQNLADPNPSALVKYLLYSHPPIEQR
                     VAHAEEWARRHEQHDAA"
     gene            complement(1625..>2043)
                     /locus_tag="VFU72_09800"
     CDS             complement(1625..>2043)
                     /locus_tag="VFU72_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423820.1"
                     /translation="GQVGSAPLSAHFGPSGHEELTYNNPFAQNLERVWFDGRLVFAVD
                     AGEVEVDPGKVKVAQEYAIVHDVELDEAGKLRRMDPVIGQYNIYDSVPGMPQYSPIWQ
                     FNYVVVPEGYEPNSLRSASACEQSGFRIARSNQFEN"
BASE COUNT          292 a          700 c          701 g          350 t
ORIGIN      
        1 ccttcgtgcg gcaggatcac cgtcacgccg gtgcggatcg gcccgacgcc cggcaccagc
       61 gcgccctgcc cggcgaccag cgtcacctgc ccgaccagca cccccggcac gtcggtgatc
      121 gcgttgagcg gacccggcgg cagcgtgccg agcaccacgc ccaggtcgcg ggcgcgcggg
      181 cgatccgact cgatctgctg gtactctgcg gtcatccgat cccctctatc ccgaacctcg
      241 gcaagcttgc tgcggtcagt tccaggcgat gtgctccaac gccggagcga gcatagcaga
      301 gcatcggatg ccggaggagg ttgccgtgag cgctcgaatg gtcgtgtttg gggccgcgtg
      361 gctcgtcgcg cgggcggcgc tgaccgctga agcggccgaa atgccggaga agggtgcggg
      421 cgagcgcgag cgcgcccggc gctacaacca tgccaagcac gtgctgctga ccctcgatct
      481 ggcgctctcg ctggtgggcg gcgccgccct ggtgctcagc ggtctcccgg gcgatgtccg
      541 gcggcgcgtc ttcgcgcgag cgtcgaaccg gctggccggg cgcacggcgg tcatcgccgt
      601 cttcacactg ggccagttca tcgtctcgct gccgctcggc tactacagcg gctacctgct
      661 ggagcatcgc ttcgacctga gcaaccagtc gcgtcgcggc tggctgtggg agcaggtcaa
      721 aggcctgctg atctcgctgc cgctggtggc cgtgctgggc aacgggctgc tctgggtcat
      781 tggccgctgg ccgcggcgct ggtggctggt cgtcagcgcg ctctcgctcc ccttcaccgt
      841 cctcttcgcc cagctcggac cggtgctgat catgccgctt tttaacaagt tcgagccctt
      901 gcgcgaccag cagctggccg agcgtctcaa ggcgctggcg gcccgctccg gcatccacgt
      961 cgcccaggtg ctggagatga acatgagcaa gcaaaccaag aaggccaacg ccttcttcgc
     1021 gggcctcggc ggcacccggc gcatcgtgct gggcgacacg ctggtcgagg acttctcgcc
     1081 cgaggagatc gaggtggtgg tggcgcacga gatcgcgcat caggcgcacc acgacatctg
     1141 gcgcttgatc gcggtgggca cggttggcac cgccgcctcg acgttcgcgg tggaacgaat
     1201 cgcgcgctgg gcgctggagc gcttcccgcg ccggttgcat ctgcattccc tggacgacgt
     1261 ggccgccctg ccgctgttgg ggattatctc cgggctggtc ggtctggtgc tgatgccgct
     1321 ggccaacgcc tactcacgcc accttgagcg gcgggccgac ggcttcgcac tcgatctgac
     1381 gcgaacgccg cgtgcgttta tcggtagcat gcgccagttg gccgagcaga acctggccga
     1441 cccgaatccc tccgcgctgg tcaagtacct gctctacagc cacccgccga tcgagcagcg
     1501 cgtcgcgcat gccgaggaat gggcgcgcag gcacgagcag cacgacgcgg cctgagcgac
     1561 caggccggcg cgcgactgag ttaccctgtg ggcgtgccgc cgaccggcgg cgttagatca
     1621 ctggtcagtt ctcgaactgg ttgctgcggg cgatccggaa accgctctgc tcgcaggcgc
     1681 tggccgagcg caggctgttc ggctcgtagc cttcaggaac gaccacgtag ttgaactgcc
     1741 agatcgggct gtactgcggc atgccgggga ccgagtcgta gatgttgtac tggccgatca
     1801 ccgggtccat gcgccgcagc ttgccggcct cgtccagctc gacgtcgtgg acgatggcat
     1861 actcctgggc caccttgacc ttgccggggt cgacttcgac ctcgcccgca tcgacggcga
     1921 agaccaggcg gccgtcgaac cagacccgct ctagattctg cgcgaacgga ttgttgtagg
     1981 tcagctcctc gtgtcccgag gggccgaagt gggcgctgag cggcgcgctg ccgacctgac
     2041 cgt
//
LOCUS       DASUBI010000510         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_900074, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000510 DASUBI010000000
VERSION     DASUBI010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900074"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..407)
                     /locus_tag="VFU72_09805"
     CDS             complement(45..407)
                     /locus_tag="VFU72_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423821.1"
                     /translation="MPGTFTDDGGIWRVGNAAGVTLVGAATQGLLDFDTMTQLLISNV
                     STQIADYHETWRTREAPDRLRITYTGQVFGVPGGGMIDQQQFGSTICALVLFAATGAQ
                     ARYTATFEAIVASLAPAR"
     gene            454..684
                     /locus_tag="VFU72_09810"
     CDS             454..684
                     /locus_tag="VFU72_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423822.1"
                     /translation="MAVAVGVGVGRGVAVGSGGGVAVGVAVGTLVAVAGGTVVGVGSA
                     RVGMGVALGGAAGSGAPRQPPSVATSRAANRA"
     gene            complement(764..991)
                     /locus_tag="VFU72_09815"
     CDS             complement(764..991)
                     /locus_tag="VFU72_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423823.1"
                     /translation="MDSHDTQQPEQSANHAGSPDSASAERDYGGDDGALEQEGGLLAA
                     PDELPAPERTPADAAQAQQEADLATGRENAS"
     gene            2572..>2884
                     /locus_tag="VFU72_09820"
     CDS             2572..>2884
                     /locus_tag="VFU72_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423824.1"
                     /translation="MRLSDLIEEFCVDCRTRRLSPKTVGWYASNLRYFLEWLREQGLA
                     DDLAALTLPTARRYSQALATRTVREATFVGNGGKRGVHALKETDRPLAANTLIGYLRS
                     LK"
BASE COUNT          395 a          950 c         1030 g          509 t
ORIGIN      
        1 agagcgccgc tggggcacca cggtggaacg ccgtcgggca cggactagcg ggcgggcgcg
       61 aggctggcga cgatcgcctc gaacgtcgcc gtgtagcgcg cctgtgcccc ggtcgccgcg
      121 aagagcacca gcgcgcagat ggtcgacccg aactgctgct ggtcgatcat gccaccacct
      181 ggcacgccga agacttggcc ggtgtaggtg atccgcagcc gatctggcgc ctcgcgggtg
      241 cgccaggtct cgtgatagtc ggcgatctgc gtgctgacgt tgctgatgag gagttgggtc
      301 atggtgtcaa agtcgagcag cccctgggtg gcggccccga ccagcgtgac accggcagca
      361 ttccccaccc gccagatgcc accatcgtcg gtgaaggtgc ccggcaccgt cgcctgacag
      421 cgtccagcgc tgtcccgaat cgtatacggc gggatggccg tcgcggtggg cgtcggcgtc
      481 ggccggggcg tggcggtggg cagcgggggc ggcgtggcgg tcggggtggc cgtcggcacg
      541 ctggtcgcgg tcgcgggcgg tacggtggtg ggtgtcggga gcgcacgggt gggcatgggc
      601 gtggcgctgg gtggggccgc cggcagcggc gcgccgcgcc agccgccgag cgtggccacg
      661 agcagggcgg cgaacagggc gtagcgcagc cgacgcagcc gcagtgctcc cttcttgccg
      721 tgccgtgacc tggggcagtc gccccggcgt ggtcgccggc gcgttagctg gcgttctctc
      781 gtccggtggc gagatccgcc tcctgctgcg cctgagcagc gtccgccggc gtgcgctctg
      841 gcgccggcag ttcgtccggg gccgctagca gcccgccctc ctgttcgagc gccccatcat
      901 cgccgccata gtcgcgttcc gctgacgccg aatcaggcga cccggcgtgg ttggctgact
      961 gttctggctg ctgggtgtcg tgagaatcca tcgtcccctc ctcgatcggc tgctgtttgt
     1021 gcggaggaga gcgcactatc ggtgccaggg cgccggctgg ctcggcgagg ccgacgctga
     1081 cgatctggtg aagagtactg gtcgaccggc gtggtccgac gggtgcagga ggacaggcgc
     1141 gtgcgagcgt catcgggtcg gcagcaacgt cagtcgaccg cacgaatcat ggcggacgag
     1201 ccgcggatgg agtcgacctc gacgaacggg tcgaggcgtg ccacgatgcg cgcccccgcg
     1261 cgccggtcag gtgcccgcgc tgagcggttg tatgcccatt gaccccttca gcgcgctgcg
     1321 ccagctggtg tgcctgctgg gggcgatgcg ccggccgcgc cggcgggcgt cgcgttcgcg
     1381 cccgcgctcg cggcgaacca ccgagccaca gtctgcacaa tcgcgctggc gcgtcccggt
     1441 cggcacgtac gtggtgccgc agtgctcgca gggacgcggc ggggccgcac cctgttggcg
     1501 gctggcgcgg atcttcgccc cacgggccgc acggcgttcc ggggtcgcac tctcactgag
     1561 atgacgccgg ccctcggcgc ggaagccgtc cgccatggcc gtcgccgcag cctgtcctgg
     1621 ccgaatactc tatccgcgtc ctaaccgggt ccggacttga cctgcagatc gggaatcatg
     1681 ggtggggcaa ttcgggaggc agatgtcctc gtctcaatcg tggatgcggc tgaagttgag
     1741 tgaccgatcg ccaatccgat gcaactgatt gaatgctttg cctgatcgat aacgtcgtcc
     1801 gccctgagat ctggctcaaa atgcgcagcg agacctgtca tggctagaag tctggctgcc
     1861 tggttcacga ggcgcgctga taggcacgag gcacgcctcg gattgaagtc gcgcgtaccg
     1921 gccaatcatg atgtcccgtc acgtggcgga cgaggaagtg tgtgggcact cgccgagacg
     1981 cgcgagcgtt ggcggctcct tggcgagcac gactatcgtg caggctcgtg ttggcaacac
     2041 tcgcgccaat ccgggacagg tctcacggat gggtccgcca gccggagcag gtatctgctg
     2101 ccgagtttcg cgggactttc tcggagattc cgaggccttt tcgcgagttc agtcggtgtt
     2161 ttccccccgt tccgccagat taggctgagt tcttagttag gtttcgagcc gattggagag
     2221 cagctgtttt gtaatcacgt gctccccgga gccccttcgg aaacccatgc gcggcggatt
     2281 tttcgcagta gcatgggctt tttgttgtcc cccttctcct cgctctgagc gtgcatcagt
     2341 cgctctccgg agtgctggaa ccgtccgaaa cggaccccgt cttgcgcccg atcggcgacg
     2401 ccttcgtccg gaaatgtcgt tcctggtctc ccgccgttcc tccgcggttc ctggtcggcg
     2461 tcgtcgctgg atggcgtcgc cggggtggtg tagctcttgg tacaaccccg tgcgagcgcc
     2521 cgctcgtcgg ggtcgcgccg ggacatacct gcctggagag gaggccccgc gatgcgcctg
     2581 tccgatctga tcgaggagtt ctgcgtcgac tgccggaccc ggcggctctc gccgaagacg
     2641 gtgggctggt atgccagcaa cctccgctac ttcctggagt ggctgcggga gcaggggctc
     2701 gccgacgatc tcgccgcgct caccctgccg accgcccgcc gctacagcca ggccctcgcc
     2761 acgcgcacgg tgcgcgaagc cacgttcgtg gggaacggcg gcaagcgcgg cgtccatgcc
     2821 ctcaaggaga ccgaccgccc cctggccgcc aacacgctca tcggctacct gcgcagcctc
     2881 aaga
//
LOCUS       DASUBI010000511         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1234119, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000511 DASUBI010000000
VERSION     DASUBI010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234119"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFU72_09825"
     CDS             complement(<1..913)
                     /locus_tag="VFU72_09825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5423825.1"
                     /translation="MSVAAGATGAPVRTTPFPTWPVYTDADLDGIAGAVRAGVWAAAD
                     GPLKQQFEARFAAFQDAAHAVAVTNGTVSLEIALAALGVGAGDEVIVPPYTFLATASA
                     VLKMNALPVFADIDPTTYCLDPAAVEAAISPRTKAIIVVHLGGHPADLDRLLAIKQRH
                     GLALIEDAAHAPDASVAGRMLGTWGLAGAFSFFSNKVLACGEGGLVATDDDEVAALAR
                     SLRSQGMTSGTWSRHTGETHSYDAVGLGYNYRLDEPRAALLHSRLRRLGEGVARRREL
                     TRAYRAKLAEIDGLVVPYEDADVECSTC"
     gene            complement(910..>3062)
                     /locus_tag="VFU72_09830"
     CDS             complement(910..>3062)
                     /locus_tag="VFU72_09830"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU5423826.1"
                     /translation="PAALAAWRAYFAARTQPLPWFNHAGWASAVAGTPLAAETLATAD
                     TLLDRTVDFTDRALGKSGLYGFHYLGWLLPLIRAYALTGDERYIAGWTRFFDQWYATR
                     DAVQGDWPGLDVIWYTLGVGVRSDPIAQALHAFRASPALPDATYQAMVKTLLGGARWL
                     DEEHQTFRHGNWQLAGCGALLNLAGFLPEFREAGAWAATARARIEEHLALDVYPDGGH
                     YERAPGYHAMCLGVLHRAAITAEQALDWPLAAHPRFAAMYDWLLALTTGGGWTPPFND
                     CQPVWSGVHLLYGYYFFARPAYKALARRLLSPETIAATLGALPPRPGRGDSADEFARA
                     PEALPADGSRVLATSKYAVLRAGWEPDGLYAALNYGPFVGHELEPHSHLAGLDFVLAG
                     WGAPLAWEAGGPNTYDDPLYYDWYRATRAHNTILAEGLHTGEDRDAELVRFAALPALD
                     LITARHEGAGGAAHERTLIFVRPAEHGAPYWLVRDRVTGVTESTWLLHGRSPWLAGER
                     LTFRSEEGPGLLVLPARPERLASSTGDTGPCKVPDPATGANVPGELHALALHAGDGRY
                     DVVLAPFAATAPEAVVEEQGDDLVVRLAGCVDRFGERQWTRERDGRLLAAACWENSSL
                     TYDDVTLVTGRNLRACSVHWTDNRLDAVVEVAARTTLALHAPGAAALRLNDIVLPLRL
                     ENDSVWVSLPCAGRYTIEVDCAHDGAQARQRRQP"
BASE COUNT          455 a         1086 c         1093 g          428 t
ORIGIN      
        1 agcaggtgga gcactcgacg tcggcgtcct catacgggac gaccaggcca tcgatctccg
       61 ccagcttggc ccggtacgcc cgggtgagct cgcgccggcg ggccaccccc tcgcccaagc
      121 gccgcagccg cgagtgcagc agcgcggccc gcggctcgtc gagccggtag ttgtagccca
      181 gccctacggc gtcgtaggaa tgcgtctcgc cggtgtggcg gctccacgta cccgacgtca
      241 tgccctggct gcgcaagctg cgcgccagcg ccgccacctc gtcgtcgtct gtggccacga
      301 gcccgccctc gccgcaggcg agcaccttgt tcgagaagaa gctgaacgca ccggccaggc
      361 cccaggtgcc gagcatgcgt cccgcgaccg acgcgtccgg cgcgtgggcc gcgtcttcga
      421 tcagggcgag gccgtgccgc tgcttgatcg ccagcaggcg atcgaggtcg gccgggtggc
      481 cgccgagatg cacgacgatg atcgcctttg tgcgcggcga gatcgccgct tctacggcgg
      541 cggggtcgag gcagtaggtt gtcgggtcaa tgtcggcgaa gaccggcagc gcgttcattt
      601 tcagcaccgc cgatgcggtc gcgaggaacg tatagggcgg cacgatgact tcgtcgcccg
      661 cgccaacgcc gagcgcggcc agcgcgatct ccagcgacac cgtgccattc gtcaccgcga
      721 cagcgtgcgc cgcgtcctga aaggccgcga accgcgcctc gaattgttgc ttgagtggcc
      781 cgtccgctgc cgcccagaca ccagcgcgca ccgcgccggc gatgccgtcc aggtcggcat
      841 ccgtatagac cggccaggtc ggaaagggcg tcgtccgcac cggcgcgccg gtcgcgcctg
      901 ccgcgacact catggttgcc tcctttgccg cgcttgcgcg ccgtcgtggg cgcagtcaac
      961 ctcgatggtg taccggccag cgcagggcag cgatacccac acgctgtcgt tctctagtct
     1021 gagtggcaga acgatgtcgt tcaatcggag cgccgccgcg ccgggtgcgt gcagcgccag
     1081 cgtggtgcgc gcggcgacct cgacaacggc gtccaggcga ttatcagtcc agtgtacgct
     1141 acaggcgcgt agattgcgcc cggtcaccag agtgacatcg tcatacgtga gggaagagtt
     1201 ctcccagcac gcggcagcca ggagccgacc atcccgctcg cgcgtccact gtcgctcacc
     1261 gaaccggtca acgcagccgg cgaggcgcac gaccaggtcg tcgccctgct cctccaccac
     1321 ggcttccgga gcggtcgcgg cgaacggcgc cagcacgacg tcgtagcggc catcgccggc
     1381 atggagcgcc agcgcgtgca gctcgcccgg tacgttcgca ccggtcgctg gatcgggcac
     1441 cttacacggc ccggtgtcgc cggtggacga cgccagccgc tccggccgcg cgggcagtac
     1501 gagcaggcca ggtccttctt ctgagcggaa cgtcagccgc tcgccggcca gccagggcga
     1561 gcggccgtgc agcagccagg tgctttccgt cacgccggtg acgcggtcgc gcaccagcca
     1621 gtagggcgcg ccgtgctcag cggggcgcac gaagatgagc gtccgctcgt gtgccgcgcc
     1681 gccagcccct tcatggcgcg cggtgatgag atcgagcgct ggcagcgccg cgaagcgaac
     1741 cagctcggcg tcgcggtctt cacccgtatg cagtccctcg gccaggatgg tgttgtgcgc
     1801 gcgggtggcg cggtaccagt cgtagtagag cgggtcatcg taggtgttcg ggccgcccgc
     1861 ctcccaggcg agcggcgcgc cccagccggc caggacgaaa tcgaggcccg cgagatggct
     1921 gtgcggttcg agctcgtggc cgacaaatgg cccgtagttc agcgcggcat agaggccgtc
     1981 cggctcccag ccggcgcgca gcacggcgta tttgctcgtc gccagcacgc gcgagccgtc
     2041 cgccggcagc gcctcgggcg cgcgggcgaa ctcgtcggcg gagtcgccgc gccctggacg
     2101 gggtggcagc gcgccgagcg tcgcggcgat cgtctccgga ctgagcagcc gccgcgccag
     2161 cgccttatac gctggccgcg cgaagaagta gtagccgtag agcaggtgga cgccgctcca
     2221 gaccggctgg cagtcgttga agggcggcgt ccagccgccg cccgtcgtca gcgccagcag
     2281 ccagtcgtac atcgcggcga agcgcgggtg cgccgccagc ggccagtcga gcgcctgctc
     2341 ggcggtgatg gccgccctgt gcaggacacc gaggcacatc gcgtggtagc caggggcacg
     2401 ctcgtagtgg ccgccgtcgg ggtagacgtc gagcgccagg tgctcctcga tgcgcgcccg
     2461 cgcggtcgcc gcccaggcgc ccgcctcccg aaactcaggc aggaagcccg ccaggttcaa
     2521 gagcgcgccg cagccggcca gctgccagtt gccgtgccgg aaggtctgat gctcttcgtc
     2581 cagccagcgc gctccgccga gcagcgtctt caccatcgcc tggtaggtcg cgtcaggcag
     2641 cgcgggactg gcgcggaagg cgtggagcgc ctgggcgatc gggtcgctgc gaacgccgac
     2701 gccgagcgtg taccagatga cgtccagacc cggccagtca ccttgcaccg cgtcacgcgt
     2761 ggcgtaccac tggtcgaaga agcgtgtcca gccggcgatg tagcgctcgt cgccggtgag
     2821 cgcgtaggcg cggataaggg gcagcaacca gccgaggtag tggaacccat agagacccga
     2881 cttgcccagc gcgcggtcgg tgaagtcaac cgtgcggtcg agcaaggtgt ctgccgtcgc
     2941 cagtgtctcc gccgccagcg gtgtgccggc gacggcgctg gcccagccgg cgtgattgaa
     3001 ccacggcagc ggctgcgtcc gcgcggcgaa gtaggcgcgc caggcagcca gcgccgccgg
     3061 cc
//
LOCUS       DASUBI010000512         5647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_41847, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000512 DASUBI010000000
VERSION     DASUBI010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41847"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFU72_09835"
     CDS             <1..377
                     /locus_tag="VFU72_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423827.1"
                     /translation="VRRHPHVFGEGVAETAGDVLKTWNEIKRQERATNVTAALAPDEP
                     FGHVPVNLPALSRAQTIYRRAHALPAGSRWPSVAQARSEDALGSELARLVAEAEAAGI
                     DAEQALRRWTRAFEEAQGTPEE"
     gene            451..2283
                     /gene="aspS"
                     /locus_tag="VFU72_09840"
     CDS             451..2283
                     /gene="aspS"
                     /locus_tag="VFU72_09840"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5423828.1"
                     /translation="MTVETTEQQRQPGERAYVTCGELRLDDVGRIVTLKGWVNRRRDH
                     GGLIFLDLRDRYGVTQIVANPAVSPEAHAVAEQARGEFVLSVTGEVSARPEGTRNPHM
                     ATGDVEVIARQVEILNTAKTPPFYINEESDVDESLRLQFRFLDLRRERMQRNLILRHQ
                     VVRFIRDYLDARDFVEVETPMLVKSTPEGARDYVVPSRVHPGTFYALPQSPQQLKQLL
                     MISGMDRYYQIVRCFRDEDLRADRQPEFTQLDLEMSFVDQDDVLRLTEGLFSDLFRVL
                     GSKPLKETPFPRLSYDEAMLRYGSDKPDLRFGMEIFDVTDLVAGSGFGVFAGAAKAGG
                     VVRGIVAPGQTQISRGQVDQLTELVRGYGAKGLAWLGLQTGENGQLTARSPIAKFLTD
                     EEIVALARRMGAQPGDLLLFVADQASVTANALGRLRSHLGRELGLIDESVYAFCWITD
                     FPMFEWNTEENRWEAMHHPFTAPLDQDISLLDSDPARVHAKAYDIVVDGLELGSGSIR
                     IHRRDVQNKIFEIMGYSQEEIEQRFGHMLRAFEYGAPPHGGIAPGIDRTVMVLAGEES
                     IREVIAFPKNASGQDLMMGAPSPVDQRQLKELSIALAPPPGERK"
     gene            2475..4025
                     /gene="pruA"
                     /locus_tag="VFU72_09845"
     CDS             2475..4025
                     /gene="pruA"
                     /locus_tag="VFU72_09845"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEU5423829.1"
                     /translation="MRGRFSNEPSLDFSVEENRAAMRAALQEVEAQLGRRYPLVIGGE
                     RRETGEWIRSVNPGNLDQLVGEVAKARPQDVEDAVAAAAEAFKQWRTMPAEGRASVLF
                     RMAGIIRRRKLELAAWMVYELDKAWDESEGELSEAIDFLEWYGRQALELAEPVTLTAI
                     PEEAVELRWVPLGVGAIIPPWNFPAAILTGMTMGPVAAGNTVVLKPASNTPVMAYKLV
                     EIMEEAGVPAGVVNFLPGSGSEIGDALVEHPKVRFVSFTGSKDVGVRIYERAAKLQPG
                     QRWLKRVSAEMGGKDAIIVDANADVAAAAEGIVTSAFGFSGQKCSACSRAIVHQDVYD
                     DLVRQVVQRTRALVKVGPGTEGEATVGAVVDAKQYRSILDYIDVGKHEARLALGGETG
                     EGNGYYIEPTIFVDVPPTARIAREEIFGPVLSIVKAKDFDEALEIANDTEYGLTGSVY
                     SRDRAHLERARQEFEVGNLYFNRKSTGALMGVHSFGGMKLSGTNTRGGGPDTLQHFME
                     LKAIGEKL"
     gene            4045..5034
                     /locus_tag="VFU72_09850"
     CDS             4045..5034
                     /locus_tag="VFU72_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5423830.1"
                     /translation="MATHGVPVSPDSTESATGSAVGSALNGFFRRGILLATQNGAVAG
                     VVRRYGMRLGAARFVAGETLDEAVPVLRSLNQQGLRTNTTLLGEGVRDAETARAVAAT
                     YRGVLDRIAAEKLQTVIALKLTHLGLDVNEDEAYQNVESIVAHAATLGNFIRIDMEES
                     ARVEPTLRIYRRLRAAGHDNVGTVLQSALYRSQQDLEDLLPSKPNLRLVKGAYLEPPS
                     VAYPKKVDVDLNYLRLAERMLPNNAFTAIATHDERIIDHVIAFTEGQGIGKDRFEFQM
                     LYGIRPQLQQDLVRRGYSVLVATPFGQEWYFYLMRRLAERPANVLFLAKNLIR"
     gene            complement(5103..>5647)
                     /locus_tag="VFU72_09855"
     CDS             complement(5103..>5647)
                     /locus_tag="VFU72_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5423831.1"
                     /translation="HALTNTAAATVFAGSLLARQGSGTRPTTATATVLSSAGLAVLLA
                     GAYLGGHQVYRYGIAVDRDAWTTPISNYTPVYDSNALAEGAATSVRTRGREIALFRQQ
                     GQVYALDNTCPHMGCPLAKGTVADGSVTCPCHGSRFRLADGALEEGPATAPAPAYDVR
                     EREGKIEVRSHTGEWLQPVQ"
BASE COUNT          975 a         1894 c         1886 g          892 t
ORIGIN      
        1 tggtgcgccg ccacccgcac gtcttcggtg aaggcgtggc cgagacagcg ggcgatgtgc
       61 tcaagacctg gaacgagatc aagcgccagg agcgagccac caatgtcacc gcggcgctcg
      121 cgccggatga gccattcggc catgttcccg tgaacttgcc ggccttgagc agggcgcaga
      181 cgatctatcg ccgcgcgcac gcgcttccag ccgggagccg atggccgagc gttgcgcagg
      241 cccggtcgga ggacgcgctt ggtagcgaac tggcgcgcct ggtggccgaa gcggaggcgg
      301 cggggatcga cgccgagcag gcgctgcgcc gctggacgcg ggcgtttgaa gaggcacagg
      361 gaacgccaga agagtaaaat tataaggctc agacgaacaa atgagccgta agcgggccac
      421 gtgggcgcgt agattgagga tgagcagcgc atgaccgttg agacgaccga gcaacagcga
      481 caacccgggg aacgcgcgta cgtgacctgc ggcgagcttc ggctcgacga cgtggggcgc
      541 atcgtcacgc tcaagggttg ggtcaatcgc cgtcgcgacc acggcgggct catcttcctc
      601 gacctgcggg atcgctacgg cgtcacccag atcgtcgcca atcccgccgt ctcgcccgag
      661 gcgcacgccg tggccgagca ggcgcgcggt gagttcgtgc tctcggtcac gggtgaggtc
      721 tcggcgcgcc cggagggcac gcgcaacccg cacatggcga ccggcgatgt cgaggtcatc
      781 gcgcgacagg tcgagatcct caacaccgcc aagacgcccc ccttctacat caacgaggag
      841 tcggatgtcg acgagtcgct gcggctgcag ttccgctttc tcgacctacg gcgcgagcgc
      901 atgcaacgca acctgatcct gcgccaccag gtcgtacgct tcatccgcga ttacctcgac
      961 gcgcgcgact tcgtcgaggt cgaaaccccg atgctggtca agagcacgcc ggagggcgcg
     1021 cgcgactacg tcgtgcccag ccgggtgcac cccggcacct tctacgccct gccgcaatcg
     1081 ccccaacaac tcaagcagct attgatgatc tccggcatgg accgctacta ccagatcgtg
     1141 cgctgcttcc gcgacgagga tctgcgcgcc gatcgccagc ccgaattcac ccagctcgac
     1201 ctggaaatgt ctttcgtcga tcaggacgac gtcttgcgcc tcaccgaggg gctcttctcc
     1261 gatttgttcc gtgtgctggg gagcaagccg ctcaaggaga cgcccttccc gcgcctgagc
     1321 tacgacgagg cgatgctgcg ctacggcagc gacaagcccg acctgcgctt cggcatggag
     1381 atcttcgacg tcaccgatct ggtggccggc agcggcttcg gcgtcttcgc tggcgccgcc
     1441 aaggcgggcg gggtcgtgcg cggcatcgtc gcgcccggtc agacgcagat cagccgcggc
     1501 caggtggatc agctcaccga gctcgttcgg ggatatggcg caaaagggct ggcatggctc
     1561 ggcctgcaaa ccggcgagaa cgggcagctc actgcccgct ctccgatcgc caagttcctg
     1621 accgatgagg agatcgtcgc cctggcccgg cgtatgggcg cccagcctgg cgacctgctc
     1681 ctcttcgtgg ccgaccaggc gagcgtgacc gccaacgcgc tcggccgcct gcgcagccac
     1741 ctgggccgcg aactggggtt gatcgacgag agcgtctacg ccttctgctg gatcacggat
     1801 ttcccgatgt tcgagtggaa cacggaggag aaccgctggg aggcgatgca ccaccctttc
     1861 accgccccgc tcgaccagga catctcgctg ctcgacagcg acccggcgcg cgtccacgcc
     1921 aaggcctacg acatcgtggt cgatggtctg gagctcggca gcggcagcat tcgtatccac
     1981 cggcgcgatg tgcagaacaa gatctttgag atcatgggct acagccagga ggagatcgag
     2041 cagcgcttcg gccacatgct gcgcgccttc gagtacggcg ccccgccgca cggcggcatc
     2101 gcccccggca tcgaccgcac cgtgatggtg ctggccggcg aggagtcgat tcgcgaggtg
     2161 atcgccttcc cgaagaacgc cagtggtcag gatctgatga tgggtgcgcc gtcgccggtc
     2221 gaccagcgcc agctcaagga actaagcatc gccctggcac ctccgccggg cgagcgcaag
     2281 tagcgttggt tcctgattct gggctcgtca ggcagcacgc agttctggct cgcggccggg
     2341 gtttccaagg ggaggcgtcc ccccggcgat ggcaccattt caggcgattg cgccacgcgc
     2401 tacactgaaa acaatagacg taccggctta ctacatcctc aggggatatg acgcagagag
     2461 gatcggtgtc cgccatgcga gggcgtttca gcaacgagcc atcgcttgat ttttccgtcg
     2521 aggagaaccg cgccgcgatg cgcgccgcgc tacaggaggt cgaggcacag ctcggccggc
     2581 gctatccgct ggtcatcggc ggcgagcgcc gcgagaccgg cgagtggatt cgctcggtca
     2641 accccggcaa cctcgatcag cttgtcggcg aggtcgccaa ggcacgacca caggatgtcg
     2701 aggacgcggt ggctgccgcc gccgaagcgt tcaagcagtg gcgcaccatg cccgccgagg
     2761 gccgcgccag cgttctcttc cgcatggccg gcatcatccg ccgccgtaag ctggagctgg
     2821 cggcctggat ggtctatgag ctcgacaagg cctgggacga gtccgaaggt gagctgagcg
     2881 aggcgatcga cttcctggag tggtatgggc gccaggcgct ggagctggcc gagccggtca
     2941 cgctgaccgc gatccccgag gaggcggtcg agctgcgctg ggtgccgctc ggcgtcggcg
     3001 cgatcatccc tccctggaac ttccccgccg ccatcctgac cggcatgacc atgggccccg
     3061 tggccgccgg caacacggtc gtcctcaagc ccgccagcaa tacgccggtg atggcctaca
     3121 agctggtcga gatcatggag gaggcgggcg tgccggccgg cgtggtcaat ttcctccccg
     3181 gctccggttc cgagatcggc gacgcactgg tcgagcaccc caaggtgcgt ttcgtctcct
     3241 tcaccggctc caaggatgtc ggcgtccgca tctacgagcg tgcggcgaag ctgcagcccg
     3301 gccagcgctg gctcaagcgc gtctcggccg agatgggcgg caaagatgcc atcatcgtcg
     3361 acgccaacgc cgacgtggcc gccgccgccg aagggatcgt gacctccgcc ttcggcttct
     3421 cgggccagaa gtgctcggcc tgctcacgcg ccatcgtcca ccaggacgtc tacgacgacc
     3481 tcgtgcgcca ggtcgtgcag cgcacccgcg cgctggtcaa ggtaggcccc ggcacggagg
     3541 gcgaggcgac ggtcggcgcc gtcgtggatg ccaaacagta ccggtcgatt ctcgactaca
     3601 tcgatgtcgg caagcacgag gcgcgcctgg cgctgggtgg cgagaccggc gaaggcaacg
     3661 gctactacat cgagccgacc atcttcgtcg atgtgccgcc tacggcgcgc atcgcccgcg
     3721 aagagatctt cggtcccgtg ctgtcgatcg tcaaagcgaa ggacttcgac gaggcgctgg
     3781 agatcgccaa cgacaccgag tacggcctca ccggctcggt ctactcgcgc gaccgtgccc
     3841 acctggagcg cgcccgccag gaattcgagg tcggcaacct ctacttcaac cgcaagtcga
     3901 ccggggcgct gatgggcgtg cactcgttcg gcggcatgaa gctgagcggc accaacacgc
     3961 gcggtggtgg gccagacacg ctgcagcact tcatggagct gaaggcgatc ggcgagaagc
     4021 tctagcgcca gaaggaggcg acgagtggct acgcacgggg tcccagtatc ccccgattca
     4081 accgaatcgg cgacgggtag cgccgtcggc tccgctctga atggcttttt ccggcgcggt
     4141 atcctgctgg ccacccagaa cggcgcggtg gccggcgtgg tccgccgcta cggcatgcgt
     4201 ctcggggctg cccgcttcgt ggccggagag acgttggatg aagcagtgcc ggtgctgcgc
     4261 tcgctcaacc agcagggcct gcgcaccaat accacgctgc tgggcgaggg tgtgcgcgac
     4321 gccgaaaccg cccgcgccgt ggccgccacc taccgtggcg tcctcgaccg catcgcggcc
     4381 gagaagcttc agacggtcat cgcgctgaag ctgacgcacc tcggcctgga cgtcaacgag
     4441 gacgaggcct atcaaaacgt cgagtcgatc gtggcccacg ccgcgacgct gggcaacttc
     4501 atccgcatcg acatggagga gtcggcgcgc gtcgagccga ccttgcgcat ctaccggcgg
     4561 ctgcgcgcgg ccggtcacga caacgtcggc acggtcctgc aatcagccct ctaccgctcg
     4621 cagcaggact tggaagacct gctgccctct aaaccgaatc tacgattggt caagggcgcc
     4681 tacctggagc cgcccagcgt ggcgtatccc aagaaggtcg acgtcgatct caactatctg
     4741 cggctggcgg aacgcatgct gcctaacaac gccttcaccg ccatcgccac ccacgacgag
     4801 cgcatcatcg accacgtcat cgcgttcacc gaggggcagg gaatcggcaa ggaccgcttc
     4861 gagttccaga tgctctatgg catccgcccg caattgcagc aggatctggt tcggcgcggc
     4921 tacagcgtgc tggtcgcgac tccgttcggc caggagtggt atttctacct gatgcgccgc
     4981 ctcgccgagc gcccggccaa cgtcctcttt ctggcgaaga acctgatcag gtagcaagac
     5041 cggcgccggc cgtgatgggt gccgggagag ggcggacccg tcacccatgc cggagtccca
     5101 cactactgaa ccggttgcag ccattctccg gtgtggctac gcacctcgat cttgccctcg
     5161 cgctcgcgga catcgtaggc cggcgcgggc gcggtggctg gcccttcctc cagcgcgcca
     5221 tccgccagcc ggaagcgtga gccgtggcac gggcacgtga cggagccgtc ggcgaccgtc
     5281 cctttcgcga gtgggcagcc catatgcggg caggtgttat cgagcgcgta gacctggcct
     5341 tgctgccgga agagcgcgat ttcgcgccct ctggttcgaa cgctagtggc cgcaccctcg
     5401 gcgagcgcat tcgagtcgta gacgggcgtg tagttgctga tcggcgtcgt ccaggcgtcg
     5461 cgatcgaccg cgatgccgta gcgatagacc tgatgaccgc cgaggtaggc tccagccagt
     5521 agcacggcaa gcccggccga cgacagaact gtcgccgtgg ccgtggtcgg gcgcgtgccg
     5581 gaaccctggc gcgcgagcag cgagcccgcg aacaccgtgg cggcggcggt gttggtcagc
     5641 gcgtgga
//
LOCUS       DASUBI010000513         7074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_818254, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000513 DASUBI010000000
VERSION     DASUBI010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818254"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="VFU72_09860"
     CDS             complement(<1..2288)
                     /locus_tag="VFU72_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU5423832.1"
                     /translation="MTIAVEALAGDARASATLDVRVLPSEAPRVSLRPEHPRIRFTTE
                     ELGQRRALLAAGQLPQLGLELRQQFQALRERADAFAAEREFTLTYTIAGEIFTPRHPL
                     PIAQPAPLPQPRGFTDYPYWTLLSRTVEARLTTLTLAYALTAEQRYAEKAKEYLLALA
                     GWQKWHEYDKATNNLSLPHFTIAAAIAYDELWALLTPEERATVRASIVQLGLRPMSYW
                     FGRRLDHNITTLMNAGMVAGYLAIGDELPYLGKFFTRPFANLQWYLAQRDAGETTEGL
                     LYTAYAVNTLLTVASQIRRATGNDELWQSPFLRTTLPELYLYFRGGQGGLANLSDAGH
                     EDGSTPLLTHLAREYAHPIAAWLISHYQRDSDAPLLLLDRDVPVTPPAALGLPTSKVF
                     LPMGWAALRTGWDDDATLLAFTASGSAAGHNHFDQNAFILNVAGDWLLTDPGYQTYSP
                     GAENFFTNATLGHNALLVNGEGQPQKGHARITDQFLTPGFDAVAGDATGAYAGALTRW
                     VRQILHVKPHYFLLVDEVTPATADDTLELLFHAQRPMVLGGRALSVGETFNSPADEIT
                     IAGEHTSVGLRVITPEAARWTYEQHPGAERFGAYLRVRLPNATHQQAVTLLQTRSGST
                     TPAYQVLAQAAPNGCWLTVDRGAERDIWWLGQDAAANARVSSGDLTGQAGQFLVVRAA
                     HTGAIQRIVLLRGTTLVAGEQTLLAADHPLWADLAYTPAGTHGTLRLATAGWVRLHAP
                     GTPLVQANRVLLPPEQVAYNPAS"
     gene            complement(2552..4369)
                     /locus_tag="VFU72_09865"
     CDS             complement(2552..4369)
                     /locus_tag="VFU72_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423833.1"
                     /translation="MATETNATMRQAAPEPMNRRAFLRRSAILGCSASAVAAFLAACG
                     SAAAPGGAATTSTTSSKAASTTAASTSAATTTAKATSAATSAATSAGSSGSGSGAATT
                     SAAGAASPGAAAPTATAIGGNHLTITAITLTYAVTPPPNDGPGLKQLNERFNIDFRPQ
                     LIPQGSYTEKLSAVIAGGDIPDIINFFDSQQMGLFYKWAGQGAFLPIDDSLLKGYQTF
                     KYVADFVWNAVTVKGKRYAIPQYYPPYALTPSIRQDWLDNLGLKMPTTYDELKQVALA
                     FTQQDPAKDGAKHYGIAMGAPINPNYAMGAYWNPGSWYHKDDKGNYQPGTITDAWKSV
                     IQFHADLYKAGALTRDFAVMKNWPDVNKEFYSGKAGIFIGAPRGMSQDYMKGLLDIYP
                     NAKPVPIYPFKAPDGSDGYAATSGYSGVSAISAKAAKDPEKVQRILALLDFNRKFYPP
                     DQQNPQNADYDWLMGHVGQGYDMKDGKVVSRDSTSNPQGLAPSSYMVGGTAWPPTDDA
                     INYQAGYTSQPRMGEWAGALQKMWSETKSYHDPSIGVISQTAQAKGGDLSQFLSDEQT
                     KMIAGQRSIDTWNDLVQEYLSKGGQQIIDETNQGIKERG"
     gene            4747..5325
                     /locus_tag="VFU72_09870"
     CDS             4747..5325
                     /locus_tag="VFU72_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5423834.1"
                     /translation="MAAHLVHREMDLRQVRERMDRAPTPRERERWHALWLLAQGWSAS
                     KVAELVERDAPTSGAWLAAFAQDGPVALALAQTGGPPARGAEAQAGLQVAVQATPSEV
                     GIALAHGNWQVVRQFIEDRCGLRLGRSACTRYLHRLGFAYKRPNKRLRKADEEQRAAY
                     VAEYVALLAEARAAGRRSSSPTRPTSAPTATW"
     gene            5334..5654
                     /locus_tag="VFU72_09875"
     CDS             5334..5654
                     /locus_tag="VFU72_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5423835.1"
                     /translation="MLKGQPALVDSACPRWGEQASDYSAVYLATGEVEHMALSGTSWS
                     ATSAAIRPQLRATHPGPLVVIGDTGPAHGGKAVREYLATPDLALRLVRLPAYRPDCNP
                     DEAI"
     gene            5673..5876
                     /locus_tag="VFU72_09880"
     CDS             5673..5876
                     /locus_tag="VFU72_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423836.1"
                     /translation="MTANTCLGSKAAVQEPMQRFFAGLPARIAEVQSRCRRKRQALAE
                     ALAAPVPESSHEALHGDPIGASV"
     gene            complement(5928..>7074)
                     /locus_tag="VFU72_09885"
     CDS             complement(5928..>7074)
                     /locus_tag="VFU72_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423837.1"
                     /translation="VLATPAWRATADELVTRAVAAQDPAGYWPEHQGPTPSYNLVYTY
                     ALGLYHLQGGAVAVLPALERALRFHELFTYPDGTLVETVDGRVKYHDHLNPAVAGFAL
                     LPRGRRYVEAIVQRALERGERWSAPHFAQLLRYWDTFPAGDAPSVFEQDHVVAELARA
                     LVVKDQDWYACLSGFTCPPVESRWGQDRQSFISVWRRAAGLIIGGGNSKNQPEWSTFD
                     ITTSDGRRCFVPAAGAVDPDTRTLTLNCEGRRLQVQLAALDARTLTLRLTAEGRAGDR
                     LATHLPVRVRPGAPFALDGEHVASEEPLSLVAAAGSHQLRQGHWQLAWTGGYTFMWPS
                     FPFNPYAKDGRSDASRPDGKPDLGAAVAILTLPLVPGEPRDVCIAHS"
BASE COUNT         1098 a         2298 c         2458 g         1220 t
ORIGIN      
        1 gacgccgggt tgtaagccac ctgctcgggc ggaaggagca ccctgttcgc ctgcacaagc
       61 ggcgttccgg gcgcgtggag gcgcacccac ccggcggtcg ccagccgcag agtgccgtgc
      121 gtgcccgccg gcgtataggc caggtcggcc cagagcgggt ggtcggcggc cagtagcgtt
      181 tgctcaccgg caacgagcgt ggtcccgcgc agcaggacga tccgctgtat ggcgccggta
      241 tgagccgcgc gcacgaccag gaactgcccg gcctgcccgg tgagatcgcc gctgctcaca
      301 cgcgcgtttg ccgcagcgtc ctgcccgagc caccagatgt cgcgttccgc gccgcgatca
      361 accgtgagcc agcagccgtt cggcgccgct tgcgccagca cctgatacgc cggtgtcgtg
      421 gaacccgacc gtgtttggag cagcgtcacg gcttgctgat gagtggcgtt gggcagccgg
      481 acgcgtaggt aagcgccgaa gcgctcggcg ccagggtgct gctcgtaggt ccagcgcgcc
      541 gcttccggcg taatgacgcg caagcccacg cttgtgtgct cgccagcgat ggttatttca
      601 tcagccggac tattgaacgt ttcacctacg ctcaaggcgc gtccgcccag taccatgggg
      661 cgttgcgcgt gaaagagcaa ttccagcgta tcgtcggcag tcgccggcgt cacctcgtca
      721 accaggagga agtagtgcgg cttgacgtgc agtatctggc gcacccaccg cgtgagggcg
      781 ccggcatagg cgccggtggc gtcgccagcg acggcgtcga agccaggcgt gaggaactgg
      841 tccgtgatcc gcgcgtggcc tttttgtggc tgtccttcgc cattcaccag cagggcattg
      901 tggccgagcg tcgcgttggt gaagaagttc tccgctcctg gagaataggt ctggtagccg
      961 gggtcggtca aaagccagtc gccggccacg ttgaggatga aggcattctg atcgaagtgg
     1021 ttatggccgg cggcggagcc gctggcagtg aaggccaaga gggtcgcgtc atcgtcccag
     1081 ccggttcgca gcgccgccca gcccatcggc aagaacacct tcgacgtggg caggccgagc
     1141 gcggccggcg gtgtgacggg cacatcgcgg tcgagcagca gcagcggcgc gtcgctgtcg
     1201 cgctggtagt gactgatgag ccaggctgcg atcggatgag catactcgcg cgccaggtgg
     1261 gtcagcagcg gcgtgctgcc atcctcgtgt ccggcgtcgg acagattcgc cagcccgccc
     1321 tgtccgcccc ggaagtagag atagagttcc ggcagggtgg tccgcaggaa cggcgactgc
     1381 cagagctcgt cattgccggt cgcccggcga atctggctcg cgacggtcaa gagggtattc
     1441 accgcgtagg cggtataaag caagccttcg gtcgtctcac cggcgtcgcg ctgggccaga
     1501 taccactgga gattggcgaa cggcctggtg aagaacttac ccaggtaggg cagttcgtcg
     1561 ccaatggcga ggtagccggc gaccatgccg gcattcatca gggtcgtgat attgtggtcg
     1621 aggcgccgcc cgaaccagta actcatcggc cgcagcccga gctggacgat gctcgcgcgc
     1681 accgtcgcgc gctcctcggg cgtcaggagc gcccagagtt catcgtaggc gatcgcggct
     1741 gcgatcgtga agtgcggcag gctgagattg ttcgtcgctt tgtcatattc gtgccacttc
     1801 tgccagcccg ccagcgccag cagatattcc ttcgccttct ccgcatagcg ttgttcggcg
     1861 gtcagcgcat aggcgagcgt gagcgtcgtc aggcgggcct ccaccgtgcg cgagagcagc
     1921 gtccaatagg ggtaatcggt gaacccgcgc ggctgcggca gcggcgcggg ctgggcgatc
     1981 ggcagcgggt ggcgcggcgt gaagatttcc ccggcgattg tataggtcaa cgtgaattca
     2041 cgctcggcgg cgaaggcgtc cgcgcgttcc cgcagcgcct ggaactgctg tcgcagttca
     2101 agaccgagct gcggcagttg ccctgcggcc aggagcgccc gccgctgccc gagttcctcg
     2161 gttgtaaagc gtatgcgtgg gtgttcaggg cgtaggctga cgcgcggcgc ttcggacggc
     2221 aacacccgca cgtcgagcgt ggcgctggcc cgcgcgtcgc cggccagcgc ttcgaccgcg
     2281 atcgtcacgt cgccggccgc ttggccggtg agttgtccat ccgtgccgac ggcaagcacc
     2341 gctggcgcgc tcgaccgaaa tgtaagccgg tccggcgtgc tggcggcccc attcagccgg
     2401 ggcgcgagcc gcgcctgttc gcccacgtcg agataccaga gcaacggtaa ctcaagctgc
     2461 atcgcgccga cattcctccg gtagccaggc gggtggcgcg ggagcgcctg gcgcgggcga
     2521 ccgccgcccg tgccggtggc tgtgaccagc cttagccgcg ttccttgatg ccctggttgg
     2581 tctcgtcaat gatctgctgg ccgcccttgc tcagatactc ttgcactagg tcgttccagg
     2641 tgtcaatgga ccgctgcccg gcgatcatct tggtctgctc gtcactgaga aactggctca
     2701 ggtcgccccc cttggcctgc gcggtctgcg agatgacccc gatgctcggg tcgtggtagg
     2761 acttcgtctc gctccacatc ttctggagtg cgccggccca ctcacccatc cggggctgcg
     2821 aggtgtagcc cgcctgatag ttgatggcat cgtcggtcgg cggccaggcc gtcccgccaa
     2881 ccatgtagga cgatggtgcc agcccctgcg ggttagatgt ggaatcccgg ctgacgactt
     2941 tgccgtcttt catgtcgtac ccctggccga catggcccat cagccagtcg tagtccgcgt
     3001 tctgcggatt ttgctgatcg ggtgggtaga acttgcggtt gaagtcgagc agggcgagga
     3061 tacgctgcac cttctccggg tctttggcgg ctttggccga gatggcgctg acaccagaat
     3121 agcccgaagt cgccgcgtag ccatccgagc catcgggcgc cttgaatgga tagattggca
     3181 ccggcttggc gttaggataa atatcgagca agcccttcat gtagtcctgt gacatgccgc
     3241 gcggggcgcc gatgaagatg ccggccttgc cgctgtagaa ttctttgttg acgtcgggcc
     3301 agttcttcat caccgcgaag tcgcgcgtga gcgcgcccgc cttgtagagg tccgcgtgga
     3361 actggatgac gctcttccac gcgtcggtga tcgtacccgg ctggtagttg cccttgtcgt
     3421 ccttgtgata ccaggaaccg ggattccagt aagcacccat ggcgtagttc gggttgatcg
     3481 gcgcgcccat tgcgataccg tagtgcttgg cgccgtcctt ggccgggtct tgctgggtga
     3541 atgcgagcgc gacctgcttg agttcgtcgt aggtcgtcgg catcttcaga ccgaggttgt
     3601 cgagccagtc ctggcggatc gagggcgtca gcgcgtaggg cgggtagtac tgcgggatag
     3661 cgtagcgctt gcccttcacc gtcacggcgt tccagacaaa gtcggcgacg tacttgaagg
     3721 tctgatagcc tttgagtagg ctgtcgtcaa tcggcaggaa ggcgccctgc cccgcccact
     3781 tgtagaagag acccatctgc tgcgaatcga agaagttgat gatgtcgggg atatcgccgc
     3841 ccgcgatgac ggccgagagt ttctcggtgt atgacccttg cgggatgagt tgtgggcgga
     3901 agtcaatatt gaatcgctcg ttgagctgct tcagccccgg cccatcgttc ggcggcggcg
     3961 taacggcgta ggtcagggtg atcgccgtga tagtcaggtg gttgccgccg atcgccgtgg
     4021 ctgtgggcgc ggcagcgccc gggctggccg cgcccgcggc tgaggtcgtg gctgccccgc
     4081 tgcctgaccc ggaggagccg gccgacgttg ccgctgacgt cgcggcgctc gtcgctttgg
     4141 cagtcgtggt ggccgccgac gtgctggcgg cggtcgtgct cgccgctttg ctgctggtag
     4201 tgctggtggt tgccgcgccg cctggcgcgg cggccgaacc gcaggcggcg aggaaggcgg
     4261 cgaccgccga cgccgaacat ccaaggatcg ccgaccgacg caggaaggcg cgccggttca
     4321 ttggctcagg cgctgcttgc cgcatggtcg cgttggtctc ggtcgccatc attcctcctc
     4381 ctgcgaaaca gttgccactg gtctccatgc tccttggcag ggtcgcctgc tccctactac
     4441 cggaatggcc aggcaaccca gcgggcggag cgcgccacga taatcgcgcc ggtgaatgcc
     4501 gcgttgacgc cgtgctcctt cggcacagcg gcgagagtat gaatagggcg gcggaggtca
     4561 cgatgctggt ttcatccggc aagcgaacta tttgaagtgt atcgcactac ctacgaatgt
     4621 caaccttgct gcgtatgcgc tctcgatgcc atgttctaaa ccgaagcgcc gatgaaccgt
     4681 acatagtcgt cgggtaaact gcctgccgtg ggcgaacggt aggcaggcgc aggggacacg
     4741 gctgagatgg cggcgcactt ggtgcatcgg gagatggacc tccggcaagt ccgtgagcgg
     4801 atggatcgcg ccccgacgcc gcgcgagcgc gagcgctggc acgccctctg gctgctggcg
     4861 cagggctggt cggccagcaa agtcgcggaa ctcgtggagc gcgacgcccc taccagcggg
     4921 gcctggctgg cagccttcgc ccaggatggc ccggtggccc tggccctcgc gcagaccggc
     4981 ggtccccccg cccgtggggc cgaggcccag gcggggctgc aagtcgcggt gcaggccacg
     5041 ccgagcgagg tcggcatcgc cctggcgcac gggaactggc aggtcgtccg ccagttcatt
     5101 gaggaccgct gcggcctccg gctcggccgc agcgcctgca cccgctatct gcaccggctg
     5161 ggcttcgcct acaagcggcc caacaagcgc ctgcgcaagg cggacgagga gcagcgcgcc
     5221 gcgtacgtgg ctgagtatgt cgccctgctg gccgaggcgc gggcggcggg acgaagatct
     5281 tcttcgccga cgaggcccac ttccgcgccg acggcgacct ggtagggaag tggctgctca
     5341 agggccagcc cgccctggtc gactctgcct gtccgcgctg gggggagcag gccagcgact
     5401 actcggcggt ctacctggcg accggggagg tcgagcatat ggcgctgagc ggcacgagtt
     5461 ggtccgcgac cagcgcggcc atccgcccgc agttgcgcgc cacccatccc gggccgctcg
     5521 tcgtgatcgg ggacaccggc ccggcgcacg gcgggaaggc ggtgcgggag tacctggcga
     5581 cgccggatct cgccctgcgc ctggtccgcc tgcccgccta ccgccccgac tgcaaccccg
     5641 acgaggcgat ctaggcctga gcacgcgagg aggtgacggc gaatacctgc ctgggcagca
     5701 aggcggcggt gcaggagccg atgcagcggt tcttcgccgg cctgccggcc cgcatcgccg
     5761 aagtccaatc ccgctgtcgc cgcaagcggc aagccctggc cgaggccctg gccgcaccgg
     5821 tgccggaatc gagtcatgag gcgctccatg gagatcccat cggcgcttca gtttaggcgg
     5881 cggcatccac cgctgtgcga ccacctgctc tggcatgggc gcgcaggtca gctgtgtgcg
     5941 atgcacacgt cacgcggctc gcccggcacc agcggcagcg tgagaattgc cacggcggcg
     6001 cccagatcgg gtttaccgtc gggccgggag gcgtctgacc ggccatcctt ggcatacggg
     6061 ttgaagggga acgagggcca catgaacgta tagccgcccg tccaggcgag ctgccagtgt
     6121 ccctgacgca gctggtgact ccctgccgct gccacaaggg acagcggttc ctcggatgcc
     6181 acatgctcac cgtccagtgc gaacggcgca ccgggccgca cgcgcaccgg gagatgcgtc
     6241 gcgagccggt cgccggcgcg accctccgcc gtcagccgga gggtgagtgt gcgcgcatcg
     6301 agcgcggcga gctgcacctg caaccggcgg ccctcgcagt tcagggtgag cgtgcgggta
     6361 tccgggtcca ccgcgccggc cgcaggcacg aagcagcgcc gcccgtcgct ggtcgtgatg
     6421 tcgaaggtgc tccactccgg ctggttcttg gagttgccgc cgccgatgat aaggccagcc
     6481 gcgcggcgcc agacactaat gaagctctgg cgatcctgcc cccagcggga ttcgactggc
     6541 ggacaggtga atccgctcag gcaggcgtac cagtcctgat cctttactac aagcgcccgc
     6601 gccagctccg ccacgacgtg gtcttgctcg aagaccgacg gcgcatcccc cgccgggaag
     6661 gtgtcccaat agcgcagcaa ctgcgcgaag tgtggggcgc tccaccgctc cccccgttcg
     6721 agcgcccgct gcacaatcgc ctcgacatac cgccgcccgc gcggcaacag cgcgaaccca
     6781 gccaccgccg gattcaggtg atcgtgatac ttcacgcgcc cgtccaccgt ctcgacgagc
     6841 gtgccatcgg gatacgtgaa gagttcgtgg aaccgcaacg ctcgttccag cgcggggagc
     6901 accgccaccg cgccgccctg gagatgatag aggccgagcg cataggtata gacgaggttg
     6961 taggacggcg tcggcccctg gtgttccggc cagtagccag cggggtcttg ggcggcgacc
     7021 gcacgggtga ccagctcgtc cgcggtcgcg cgccacgccg gtgtcgcgag cacc
//
LOCUS       DASUBI010000514         8411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_790259, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000514 DASUBI010000000
VERSION     DASUBI010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790259"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFU72_09890"
     CDS             complement(<1..581)
                     /locus_tag="VFU72_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5423838.1"
                     /translation="MDFEFTKEQLQVRDMVREWANRAVAPYIQEWDAKQEKHPEVLYQ
                     MGELGILGLPIPERYGGSGLDYVSYGLACEELEAVDTFLRVVMSVHVGLNSLTLLQWA
                     SEEQKQRWLVPQAEGKKLAAFGLTEPGAGSDAGNIQTRARRDGDHYVLNGEKTWISLA
                     NTADHFLVFATVDPAKKHNGLVALMVERGGEGLT"
     gene            854..1594
                     /locus_tag="VFU72_09895"
     CDS             854..1594
                     /locus_tag="VFU72_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5423839.1"
                     /translation="MSESPDEKTKEVLPETSAPTAYDGGSAGGEARADRELIEALRRG
                     DEAAFVALVQRYHTALVRLAATFVRDRALAEEAAQDTWLGVLRGIDRFEGRSSLKTWI
                     FSILIKRAKTQGQRSSRSVPFSALDPFEPGPDEPAVDPSRFHPANHRWPGHWVSVPLD
                     WGDNPEDVLVSKETGQRVMHTIDMLPAGQRAVITLRDVEGWTSGEVCEVLAISEINQR
                     VLLHRARSRVRREFERCVAESRDSLDRS"
     gene            1598..1873
                     /locus_tag="VFU72_09900"
     CDS             1598..1873
                     /locus_tag="VFU72_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5423840.1"
                     /translation="MEHPDELSCQELVELVTDYLEGALTPTMLALVRAHLDSCRGCRR
                     YLEQIRQTIELLGQLPDEGITPEAQEELLRAFHDWKTSRAGSPPQSP"
     gene            2245..3453
                     /locus_tag="VFU72_09905"
     CDS             2245..3453
                     /locus_tag="VFU72_09905"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEU5423841.1"
                     /translation="MPWKSDEIQTIRISRRRMLGGAAASVAALALSAAGGPLAAAAGS
                     PNPAGQSVGYLYVNGNTTGVNTSDGFSRRPDGSLTPLPGSPFSIGGAGTGAALASQGA
                     LALSWNGRYLLAVDAGSDQISVARINPDGSLTAVPGSPFPSNGVKPVSITVHHDMVFV
                     ANAGAGGANYTGCVLDSDGQLTPLAGSTFAVPDGAGLGLVLFDQSGRHLVGMRVNPSL
                     IDSFIVGSDGHLTAAPGSPFAAEAAGPFGSAFRPGTSPAQLFVSNAHAGPGQGSVSAF
                     EVANNGDLSPLPGSPYPNGETAPCWVALSPDRRYLFAANTGSSSISSYRIAAGGSLSL
                     VGSLPLRGGSGLGIFDLGLDPSGRYLYELEGGVQKVAALTARGGTLAELSSSPVALPA
                     GSIPGGIVVV"
     gene            complement(3469..4392)
                     /locus_tag="VFU72_09910"
     CDS             complement(3469..4392)
                     /locus_tag="VFU72_09910"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEU5423842.1"
                     /translation="MTLNAVGSEIGAAGRTRSRRLGALLEAARPRQWTKNLIVFAPLV
                     FAGDLTHISSLLRVGAAFVLFCVTSAAVYLFNDARDAEADRRHPLKQQRPIAAGRLAP
                     GTALRASGLLALGAVGAAALLDPRLAAVLAGYGLLMAAYTLWLKRLVLLDVLAIAAGF
                     VLRAAAGAVVIAVPLSLWLYACTVLLSLFLGFGKRRHELALLRATAGAHRTSLAAYAP
                     ALLDRLIVLTAGATILAYTLYTVAAPTLPANHAMLLTVPFVLYGLGRYLYLVYKQGHG
                     GAPERVLLEDWPLLASVLLWGAAAIVILYIF"
     gene            complement(4389..4961)
                     /locus_tag="VFU72_09915"
     CDS             complement(4389..4961)
                     /locus_tag="VFU72_09915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5423843.1"
                     /translation="MRWRIEKHETLGSTMDLAAARAVAGEPAGLVIWAEQQSAGRGRL
                     GRVWQADAGTSLLVTLLLRPTLSVVRDPELSREIAAAAARAIQRVTGFQPDIKAPNDL
                     MAGGRKLAGILCQTSLRGEEVDYLLVGIGLNVNLPAARLPLPTATSLLVETGRPHDRD
                     ALLAAILDEVLVMPGLAPGEDEPGSADWAG"
     gene            5141..5752
                     /locus_tag="VFU72_09920"
     CDS             5141..5752
                     /locus_tag="VFU72_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423844.1"
                     /translation="MAMAVQLTASADLEAAEPGIDESLRRLEDELNALAPAEVAAVLE
                     AVARALETPAQPAPAVVAALSGGRTFSPTERVVAEFEVLERSFRRRQQLLAGALSTTQ
                     VATLLKTSRQTPHDRIASGSLLAVMDRGALRFPAWQFDPDGPDGVVAGLPRVLRALEV
                     SPMVKVSWLTRANSVLDGATPLAALKAGRGEEVLALARAVGLD"
     gene            5754..6398
                     /locus_tag="VFU72_09925"
     CDS             5754..6398
                     /locus_tag="VFU72_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423845.1"
                     /translation="MVVVSPPPPARPVRPRFSAIPAGEKLVRIFDPTRHGTTALTFRA
                     NGPRKRFDHHRGTTHDRAPCDDPERAIYYAAWSDRAPAGAFSSCFVEIFGDTGLVELD
                     HRAVALPTLTRPLHLLDLRGVGAMRAGTVAAITACDYRLSQRWACYFYEDTDDFDTID
                     GISYHNAHNGEPALALFERARDALICPPEDVMRLDNPALRPLVLDIMRQNNLSF"
     gene            complement(6464..7426)
                     /locus_tag="VFU72_09930"
     CDS             complement(6464..7426)
                     /locus_tag="VFU72_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU5423846.1"
                     /translation="MADYDLVTFGETMIRLSTPLGQRLETAPALDAGIGGAESNVAVA
                     LARLGRRVAWFSVLPRNAFGRRIASELRRHGVDERFIHWQDTGRVGVYYLDTGSPPRP
                     THVLYDRAGSTLATCPPDTVDTTLPRRARLLHLTGITPALSPSAAEITRRLAVSASES
                     GVPLSFDVNYRALLWPPEEAAEILAPFCAAAAILFCGRADARVLWGLEGSDEEVLRAL
                     AQRFGAALTVLTLGEQGALALRRDGQLLRQAGVPVSVVDRVGAGDAFAAGFLHGYLDG
                     DLREALRLGVSLAALKMTIHGDLSLISGRELAEWSDPHGRGIIR"
     gene            complement(7451..7945)
                     /locus_tag="VFU72_09935"
     CDS             complement(7451..7945)
                     /locus_tag="VFU72_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5423847.1"
                     /translation="MLQPDELDRQIIRLLRADGRRSNREIARRLEVPEATIRYRVRRL
                     IESGILKITASVDPEHLGYALTAMISVQVEPERISEASQEIAAMPEVMWTAITAGAHD
                     VMLMASFRNQDEMFDFLTNRLARVPGTLRTETSVALRVLKKQHEWATELTSKVEAGVG
                     ANAD"
     gene            complement(8220..>8411)
                     /locus_tag="VFU72_09940"
     CDS             complement(8220..>8411)
                     /locus_tag="VFU72_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423848.1"
                     /translation="LTPREQDVLNLMARGYRNKEIAHDLHITERTVKFHANAIYRKLD
                     VGGRTEAVSKALQRGMVKS"
BASE COUNT         1285 a         2932 c         2888 g         1306 t
ORIGIN      
        1 gtgagtccct caccgccgcg ctcgaccatc agcgccacca ggccgttgtg cttcttggcg
       61 gggtcgacgg tggcgaagac gaggaagtgg tcggcggtgt tggccagcga gatccaggtc
      121 ttctcgccgt tgagcacgta gtgatcgccg tcgcgccggg cgcgcgtctg gatgttgccg
      181 gcgtcggagc cggcgcccgg ctcggtcagc ccgaaggcgg ccagcttctt gccctcggcc
      241 tgcggcacca gccagcgctg cttctgctcc tcgctggccc actgcagcag cgtcaggctg
      301 ttgaggccga cgtggacgct catcaccacg cgcaggaagg tgtcgaccgc ctccagctcc
      361 tcgcaggcca ggccgtagct gacgtagtcg agcccgctgc cgccgtagcg ctcggggatc
      421 ggcagcccga ggatgcccag ctcgcccatc tggtagagga cttcggggtg cttctcctgc
      481 ttggcgtccc actcttgaat gtagggcgcc accgcgcgat tggcccattc gcggaccatg
      541 tcgcgcacct gcaactgctc tttggtgaac tcgaaatcca tcgtgccctc ccgcggctat
      601 cggcggagac tccctcaccc ccggcccctc tccccggcgg gcgtgagggg agacgagaag
      661 aggaggggcg gggtgccggc ccgtgcggcg cttgcgagta tagcagagga ctttcgcggc
      721 tccgctccgt gcagacgcgc ggtggcgctc gcgcgggcac ccccgcgggc gttcaggccg
      781 gcggcgtata atcctggcca caattgattg tagctacgca acgtcggcca cgcccttccg
      841 cggggagcgc gtcgtgagtg agtcgcctga tgagaagacc aaagaggtct tgccggaaac
      901 ctcggcgccg accgcgtatg acgggggcag tgccggtggg gaggcaagag ccgaccggga
      961 gctgatcgag gcgctgcgtc gcggcgacga ggccgcattc gtggcgctcg tccagcgcta
     1021 ccacacggct ctggtacgcc tggcggcgac gtttgttcgc gaccgtgccc tggccgagga
     1081 ggctgcgcag gacacctggc tcggcgtgct gcgcgggatc gatcgtttcg aggggcgctc
     1141 ctcgctgaag acctggatct tcagcatctt gatcaagcgc gcgaagacac agggccagcg
     1201 ctcaagccgc tcggtaccgt tctcagccct cgatccgttc gagcccggac cggacgagcc
     1261 cgcggtcgat ccgtcgcgct ttcacccggc gaaccaccgc tggcccggcc attgggtgtc
     1321 ggtgccgctc gactggggag acaaccccga ggatgtactc gtctccaagg agaccgggca
     1381 acgcgtcatg cacaccatcg acatgctgcc ggccggccag cgcgcggtca ttactctccg
     1441 cgatgtcgag ggttggacgt ccggtgaggt ctgtgaggtg ctcgccatct ccgagatcaa
     1501 tcagcgggtg ctcctgcacc gggctcgttc tcgcgtgcga cgcgagttcg aacggtgtgt
     1561 cgccgagtcg cgcgacagcc tggatcggag ttagggtatg gaacaccccg acgaactgag
     1621 ttgccaggaa ctggtcgagc tggtcaccga ctacctcgag ggcgcgctca cgcccaccat
     1681 gctcgcgctc gtcagggcac accttgattc ctgtcgaggc tgccggcgct atctggagca
     1741 gatccggcag acgatcgaac tactgggcca gcttcccgat gaggggatca cgcctgaggc
     1801 tcaggaggag ctactccgag ccttccacga ctggaagacc agtcgtgccg gctcacctcc
     1861 ccaatccccc tagagcgccg gccaactaga gacaggggac ggcgagagga cattggcctg
     1921 acggtcgcta ctacccgacg ccagggatct cttcggtgtt acccaggtac cgggcgttac
     1981 gtgaccggcg ctctagcgag cggtcagcac gcgtgggcga tcgcgaaacc gtcagattgg
     2041 cagacccgag gatcggcccc gagccatcaa tcgctgacgc ccatcgtgca gtccaccgct
     2101 cagctcaccg ttccagccgc gcctggtcga tccgcttcct gtaacgaacc agcctccacc
     2161 aggaccaaga gatgtgcggc gcggttccgc ttgcgaccag ctcggtccaa cccgccgcac
     2221 atcaaggcag gaaggagagg ccctatgcct tggaagagcg acgagatcca gaccatacgg
     2281 atctcgcgtc gacggatgct cggaggcgcg gccgcctctg ttgccgcgct cgcactgtcg
     2341 gccgctggag gcccgcttgc cgctgccgcc ggcagcccga acccggcggg gcagtccgtc
     2401 ggctatctct acgtcaacgg caacacgacc ggcgtcaaca ccagcgacgg cttctcacgg
     2461 cggccagatg gctccttgac cccgcttccc gggtcgccct tctcaatcgg cggagccggc
     2521 accggcgcgg cgctggcctc ccagggcgcc ctggccctct cgtggaacgg gcgctacctg
     2581 ctggccgtcg acgcaggcag cgaccagatc tcggtcgccc gcatcaaccc cgacggctcg
     2641 ctcacggcgg tccccggcag cccgttcccc tcgaacggcg tcaagccggt cagcatcacc
     2701 gtccaccacg acatggtctt cgtcgcgaac gccggggccg gcggcgccaa ctatacgggc
     2761 tgtgtgcttg atagcgacgg gcagttgacg ccgctcgcgg gctcgacgtt cgcggtgccg
     2821 gatggcgcgg gcctcggcct ggtgctcttc gatcagagcg gccggcatct cgtcgggatg
     2881 cgggtcaatc cgagcctcat cgacagcttt atcgtcggct cggacggcca cctgaccgcc
     2941 gcgcccggct cgcccttcgc ggccgaggcg gctggacctt tcggcagcgc gttccgcccg
     3001 ggcacctccc cggcccaact cttcgtatcg aacgcccacg ccggtccggg ccagggaagc
     3061 gtctccgcgt tcgaggtcgc caacaacgga gatctgtccc cgttgcccgg ctccccctac
     3121 ccgaatggcg agaccgcacc ttgctgggtg gcgctctcgc ccgaccgccg gtacctgttc
     3181 gcggcgaata ccggcagcag ctccatctcg agctatcgga tcgcggcggg cggctcgctg
     3241 tcgctggtcg gttcgctgcc gctccgtggc ggctccggtc tgggcatctt cgatctcggg
     3301 ctggatccga gcggcagata cctctatgag ctcgagggcg gcgtgcagaa ggtcgctgcc
     3361 cttaccgcaa gaggtggaac cctggccgag ctgagctcct cgcccgtcgc gctcccggct
     3421 ggttcgatcc cgggagggat cgtcgtcgtc tagccgagag ccgggcgttc agaagatgta
     3481 gaggatcacg atcgcggcgg cgccccagag gaggacgctg gcgagcagcg gccagtcctc
     3541 caggagcacc cgctcgggcg cgccgccgtg cccctgcttg tagaccaggt agagatagcg
     3601 gccgaggccg tagaggacga agggaaccgt cagcagcatg gcatggttgg ccggcagcgt
     3661 cggcgcggcc acggtgtaga gcgtgtaggc caggatcgtc gcccccgccg tcagcacgat
     3721 caggcggtcg agcagcgccg gcgcgtaggc ggccaggctg gttcgatgcg cgccggccgt
     3781 cgcccgcagc agcgccagct cgtggcgccg cttgccgaag ccgaggaaga gggagagcag
     3841 cacggtgcag gcgtagagcc agagcgagag cggcaccgcg atgacgaccg cgccggccgc
     3901 cgcgcgcagc acgaagccgg ccgcgatcgc cagcacgtcg agcagcacca gccgcttcag
     3961 ccagagggtg taggccgcca tcagcagccc gtagccggcc agcaccgccg ccagccgcgg
     4021 gtcgagcagc gccgccgcgc ccacggctcc gagcgccagc aggccgctcg cgcgcagcgc
     4081 ggtccccggc gccagccgtc cggcggcgat gggccgctgc tgtttgagcg ggtggcgccg
     4141 gtcggcctcg gcgtcgcgcg cgtcgttgaa gagatagacg gcggcgctgg taacgcagaa
     4201 gagaacgaac gccgcgccga cccgcagcag cgacgatatg tgcgtcagat cgcccgcgaa
     4261 gaccagcggc gcgaagacga tcaggttctt ggtccattgc cgcggccgcg ccgcctccag
     4321 cagcgcgccc agccggcggc tacgggtgcg ccccgcggca ccgatctcgg agccgaccgc
     4381 gttcagtgtc atcctgccca gtccgcgctg ccgggctcat cctcacccgg cgccagcccc
     4441 ggcatcacca gcacttcatc aaggatcgcg gccagcaggg cgtcgcggtc gtggggccgc
     4501 ccggtctcga ccagcaggct ggtggcggtc ggcagcggaa ggcgcgcggc agggaggttg
     4561 acgttgaggc cgatgccgac cagcaggtag tcgacctcct cgccacgcag gctggtctga
     4621 cagaggatgc ccgccagctt gcggccgccc gccatcaggt cgttgggcgc cttgatatcc
     4681 ggctggaagc cggtgacccg ctggatcgca cgggcggcag cagccgcgat ctcccggctc
     4741 agctcggggt cgcggaccac ggagagggtc ggccgcagca gcagcgtcac cagcaagctg
     4801 gtgccggcgt ccgcctgcca gacgcgtccc agccgcccac ggccggcgct ctgctgctcg
     4861 gcccagatca ccagcccggc aggctccccg gccacggcgc gcgccgccgc caggtccatg
     4921 gtcgagccga gcgtctcatg tttttcgatc cgccagcgca cgccgtccac ccatcgtctc
     4981 gcgtcatcac cacccaggcg cttcaggcct ccggcaagca gcatgatcct acaacgcggc
     5041 gcggtgcatc gctggtttgg cccacgaccg gcggcaccga tcttgacatc tcttacagct
     5101 gcggtatgat gagatcacgt tctgggtgcg gaggtgcggc atggcgatgg cagtgcagct
     5161 cacggcaagc gcggatctgg aagcggctga gcccggcatc gacgagagcc tacggcgctt
     5221 ggaggatgag ttgaatgccc tcgcgcccgc ggaagtcgcc gccgtgctgg aggccgtcgc
     5281 gcgcgcgctg gagacgccgg cccagccggc tccggccgtc gtcgcagctc tgagcggagg
     5341 ccgcaccttc agcccaaccg agcgcgtggt ggcggagttc gaggtgctcg agcgctcgtt
     5401 ccggcgccgc cagcagttgc tggccggtgc gctgtcgacc acccaggtgg cgacgctgct
     5461 caagacatcc cggcagacgc cgcatgatcg catcgccagc gggtcgctgc tggcggtgat
     5521 ggatcgcggc gcgctgcgct tcccggcttg gcagttcgat cccgatggtc cggacggcgt
     5581 cgtggccggg ctgccgcgcg tgctgcgcgc gctcgaggtc tcgccgatgg tgaaggtgag
     5641 ttggctgacc cgggccaact ccgtgctcga cggcgcgacg ccgctcgctg ccctgaaggc
     5701 gggccggggc gaggaggtgc tggctctggc gcgcgcggtg gggctggatt agcgtggtgg
     5761 tggtgagccc cccaccacct gctcgcccgg tgagaccccg cttcagcgcc attcccgccg
     5821 gagaaaagct ggtccgcatc ttcgacccca cgcggcacgg cacaacggcg ctcaccttcc
     5881 gcgccaacgg tccgcgcaag cggttcgacc accatcgcgg cacgactcac gatcgagcgc
     5941 cgtgcgacga tcccgagcgg gccatttatt acgccgcctg gtccgatcgc gcacccgcgg
     6001 gggccttctc cagttgcttc gtcgagatct tcggtgacac cggtctcgtt gagcttgatc
     6061 atcgcgcggt cgcactgccg acgctgacgc gcccactcca tctcctcgat ctgcgcgggg
     6121 ttggcgccat gcgcgcggga acggtggcgg cgatcaccgc ctgcgactac cggctgtcgc
     6181 agcgctgggc ctgttacttc tacgaggaca ccgatgattt tgatacgatc gacgggattt
     6241 cctaccacaa cgcccacaac ggcgagccgg ccctggcgct gttcgagcgc gccagggacg
     6301 cgttgatctg ccctcccgaa gatgtgatgc gcctcgacaa ccccgcgctt cgccccctgg
     6361 tgctcgacat tatgcggcag aacaacctct ccttttgaac ggcgtctcgc gtgcgagacc
     6421 gcgaacgcgg tgtcgcccgg gccgcgtgat cccattggat ggttcagcgg atgatgccgc
     6481 ggccgtgggg gtcggaccac tcagccaatt cccggccgct gatcaggctg agatcgccgt
     6541 ggatggtcat cttcagcgcg gccaggctga cgcccaggcg cagcgcctcg cgcaggtcgc
     6601 catcgagata gccgtgcagg aagccggcgg cgaaggcgtc accggcaccg acgcggtcga
     6661 cgacggagac cggcacgccc gcctggcgca agagctgccc gtcgcgcctc agcgccagcg
     6721 cgccctgctc gccgagcgtg agcacggtga gggcggcgcc gaagcgctgg gctagcgcgc
     6781 gcagcacctc ctcgtcgctg ccctccagac cccagagcac gcgggcgtcg gcgcggccgc
     6841 agaagaggat ggcggccgcc gcgcagaagg gagccaggat ctcggcggcc tcctcgggcg
     6901 gccagaggag ggcgcggtag ttgacgtcga agctgagcgg aacgccggac tcgctcgcgc
     6961 tgacggccag gcgacgggtg atctcggcgg cggaaggact gagcgccggg gtgataccgg
     7021 tcagatgcaa gagccgtgcg cggcggggga gtgtggtgtc gaccgtgtcc ggcgggcagg
     7081 tggccagcgt cgagccggcg cggtcataga ggacgtgagt ggggcgcggc gggctgccgg
     7141 tgtcgaggta gtagacgccg acgcggccgg tgtcttgcca gtggatgaag cgctcgtcga
     7201 cgccgtggcg gcgcagctcg ctggcgatgc gccggccgaa ggcgttgcgg ggcaggaccg
     7261 agaaccaggc gacgcgccgg ccgagacgcg ccagcgccac ggccacgttt gactccgcgc
     7321 cgccgatgcc ggcgtcgagc gccggcgcgg tctcgagccg ctgaccgagc ggcgtcgaga
     7381 gccggatcat cgtctcgccg aaggtgacga gatcgtagtc ggccacggtc ctgtcccgct
     7441 ctcgcacgag ctaatcggcg ttcgccccga cccccgcttc aaccttgctg gtcagctcgg
     7501 tggcccactc gtgctgcttc ttgagcaccc gcagcgccac ggaggtctcg gtgcgcagcg
     7561 tgccgggcac gcgcgccagg cgattggtga gaaagtcgaa catctcgtcc tgattgcgga
     7621 acgaggccat cagcatcacg tcgtgcgcgc cggccgtgat cgccgtccac atcacctccg
     7681 gcatcgccgc gatctcctgc gaggcctcgc tgatgcgctc gggctcgacc tgcacgctga
     7741 tcatggcggt cagggcgtag ccgagatgct cgggatcgac cgaggcggtg atcttgagga
     7801 tgccgctctc gatcaagcgc cgcacgcggt aacggatcgt cgcctccggc acctctaacc
     7861 ggcgcgcgat ctcgcgattg ctccgacggc cgtccgcccg cagcaggcga atgatctggc
     7921 gatccagctc atccggctgc agcatccggg cccgtcgtga actctgaacg gctgtcatgt
     7981 cgggatcacg ctcctctcct agcatcacat cgcgtcaggc ccacggcgcg accgccgtgg
     8041 gctggattca gcagaccaga ccagtagtaa cacgtgccga acgttcatat tcggtcacgt
     8101 gagaaccgat tcctgcgcaa tcgcataccg tcgcgccgga ggaatgcgat ctgcccacaa
     8161 gtgtacacga gcggattcgc agagaagcgc gaacgcgggc gtgctaccac aaattagtac
     8221 tagctcttga ccataccccg ctgcagcgcc ttgctcaccg cctcggtccg cccgcccaca
     8281 tcgagcttac gatagatcgc gttggcgtgg aacttcaccg tgcgctccgt gatgtgcagg
     8341 tcgtgggcga tctccttgtt gcggtagccg cgcgccatca ggttcagcac gtcctgctca
     8401 cgcggggtca g
//
LOCUS       DASUBI010000515         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_452426, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000515 DASUBI010000000
VERSION     DASUBI010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452426"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VFU72_09945"
     CDS             complement(<1..1053)
                     /locus_tag="VFU72_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5423849.1"
                     /translation="MSELTELTAAEARRRLDRRELSAEELTAAHLARIARLEPGVHSL
                     IHVMEGVAREQARQADRRIGRGESAPLTGIPVALKDILCTVDAPTTAASRMLTNYESV
                     YDATVVARLRAQGAVFVGKTNTDEFAMGSSTENSAYGPTHNPWNLDCVPGGSSGGSSA
                     AVAAGEAIVSLGSDTGGSIRQPAGFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPFAR
                     TVEDAALLLSAIAGVDPYDSTSVDVPVPDYASLLGGDLRGMRLGVAREYQIEGMDPEV
                     QRTVAAAVEQLRALGAELVEVSLPHTKYALATYYIVAPAEASANLARYDGVKYGYSVP
                     ADTLLESYLLTRGQGFG"
     gene            complement(1082..1375)
                     /gene="gatC"
                     /locus_tag="VFU72_09950"
     CDS             complement(1082..1375)
                     /gene="gatC"
                     /locus_tag="VFU72_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5423850.1"
                     /translation="MTLTPAEVDHVAMLARLGLSAEERELMREQLSSILEHIQALEEL
                     DTAAIPPTAQVITLQNVMRADEVRPSLPVESVLQNAPQTEDQMFKVNAVLDES"
     gene            1562..>2478
                     /locus_tag="VFU72_09955"
     CDS             1562..>2478
                     /locus_tag="VFU72_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423851.1"
                     /translation="MPRGAPTPRNRTELPRLLALALALLLCLPACVDDLDQSAIQPGQ
                     ELAATTTAAAAASPRPVHHTAPVNVPARRRIYFTQRGDLWQLPVDSAPAPVLRDKRIL
                     AFAPSPDASQVAVVFQGDNGAEEFAVVRDDSTVALHVPLVDQSGTPIAGAAGVNALAW
                     SPRGDSIALARDDGSLSTVGIDGKVQQLTAPHPGGHPGALVWAPDGQTLAYLDPALRA
                     KAASLYSVPLNGGAPRVLAESGGGERPVLAAAWLDAASLIYVQSKPGDIPHGGDLFQL
                     DLAGGQPALLVSTGQFAPVAGVMALAVSE"
BASE COUNT          343 a          860 c          918 g          357 t
ORIGIN      
        1 cccgaaaccc tggccgcggg tcagcagata gctctccagc agggtgtcgg ccggcacgct
       61 gtagccgtac ttgacgccgt cgtagcgcgc caggttggcg ctggcctcgg cgggggcgac
      121 gatgtagtag gtcgccagcg cgtacttggt gtgcggcagg ctgacctcga ccagttcggc
      181 gcccagtgcg cgcaactgct cgaccgccgc ggcgaccgtg cgttgcacct ccgggtccat
      241 cccctcgatc tgatactcgc gcgccacgcc caggcgcatg ccgcgcagat cgccgcccag
      301 cagcgaagcg tagtcgggca ccggcacgtc gaccgaggtc gagtcgtagg gatccacccc
      361 ggcgatggcc gagagcagca gcgcggcgtc ctcgaccgtg cgcgcgaagg ggccgatctg
      421 atcgaggctg ctggcgaagg cgatcaggcc gaagcgggag acgcgcccgt aggtgggctt
      481 catgccgact acgccgcaga agccagccgg ctggcggatc gagccgccgg tgtcggaccc
      541 gagcgagacg atcgcctcgc cggcggccac ggcggccgag gagccgccgc tggagccgcc
      601 gggcacacaa tccaggttcc aggggttgtg cgtcggaccg taggccgagt tctcggtcga
      661 ggagcccatg gcgaactcgt cggtgttggt cttgccgacg aagaccgcgc cttgcgcgcg
      721 cagccgggcc accacggtcg cgtcgtagac cgactcatag ttggtgagca tgcgcgaggc
      781 ggcggtggtg ggcgcgtcga cggtgcagag gatgtctttg agcgccaccg ggatgccggt
      841 caagggcgcg ctctccccgc gcccgatacg ccggtcggcc tggcgcgcct gctcgcgggc
      901 caccccctcc atcacgtgga tgaggctgtg gacgcccggc tccaagcgcg cgatgcgcgc
      961 taggtgggcg gcggtcagct cctcggcgct cagctcgcgc cggtcgagcc tccggcgcgc
     1021 ctcggcggcg gtcagctctg tcagttcgct catcgcaggt ctcgctctca gaactccggg
     1081 cttacgattc gtcgagcacg gcgttgactt tgaacatctg gtcctcggtc tggggggcgt
     1141 tctgcagcac cgactcgacc gggagcgagg ggcgcacctc gtcggcacgc atgacgttct
     1201 gcagggtgat cacctgggcg gtaggcggga tcgcggcggt gtccagctcc tccagcgcct
     1261 ggatgtgctc caggatcgag gagagctgct cgcgcatcag ctcgcgctcc tcggcgctca
     1321 ggccgagccg ggcgagcatg gcgacgtggt cgacttccgc cggggtaagt gtcacggtgt
     1381 ggaacccttc aagcctcgtc gcgggcgagc gcgggtgcgt cgggccgtgc cggtttggca
     1441 ccgagcgagt gtagcacagg ggccagcggc ccgccaggaa gcatcgtccg tacgcgcgct
     1501 cgaagcgcgg cgcaggcgag tgctagaatc agcgcgtcga ctgagccgtg ggaggagcga
     1561 cgtgccgcga ggcgccccca ccccgaggaa ccgaaccgaa ctgccgcgtc tgctggcgct
     1621 ggcactcgcg ctgctgctct gcctgccggc ctgcgtggac gacctcgatc agagcgcgat
     1681 ccagccgggg caggagctgg cggcgacgac gacggccgcg gcggccgcct cgccgcggcc
     1741 ggtccaccac acggccccgg tcaacgtgcc ggcgcggcgg cgcatctact tcacccagcg
     1801 cggcgatctc tggcagctgc cggtggatag cgcgcccgcc ccggtgctgc gcgacaagcg
     1861 catcttggcc ttcgcgccct cgcccgacgc cagtcaggtc gcggtcgtgt ttcaaggaga
     1921 caacggagcc gaggagttcg ccgtcgtgcg cgacgatagc acggtggcgc tgcacgtgcc
     1981 gctggtcgac caatccggca cgccgatcgc cggggcggcc ggggtgaacg cgttggcctg
     2041 gtcgccgcgc ggagacagca tcgcgctggc gcgcgacgac ggctcgctca gcacggtggg
     2101 gatcgacggc aaagtgcagc agttgaccgc gccgcacccc ggtggccacc cgggcgcgct
     2161 ggtctgggcg cccgatgggc aaacgctggc ctatctcgac cccgcgctgc gcgcgaaagc
     2221 ggcctcgctc tacagcgtgc cgctgaatgg cggcgcacct cgggtgctgg ccgagagcgg
     2281 cgggggcgag cgtccggtgc tggcggctgc ctggctcgac gcggccagcc tcatctacgt
     2341 ccagagcaag ccgggcgaca tcccgcacgg tggcgacctc ttccagctcg acctcgccgg
     2401 cgggcagccg gccctcctgg tcagcaccgg gcagttcgcg cccgtggccg gcgtgatggc
     2461 gctggcggtc tcggagaa
//
LOCUS       DASUBI010000516         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_28603, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000516 DASUBI010000000
VERSION     DASUBI010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28603"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFU72_09960"
     CDS             <1..679
                     /locus_tag="VFU72_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU5423852.1"
                     /translation="PAKTLLTWEKELLGCYLSSHPLNDYLAQIRQRGMVQLAEIDEES
                     VGQTVELIALVAGLRKLTTKTNRTMAVVELEDQSGSIEAVFFPETYDAAAELLELDAI
                     VHLSARADQRNDRIQLVANRLTRAALDRRVPQLVREVHLRLPRGTSRAELNKMHRLRT
                     LLDEFPGDDQVVLHLPARGAEVALLAGLRIDWCGDLSAALGELLGEEHVGVVDREELV
                     PAAALAD"
     gene            936..1814
                     /locus_tag="VFU72_09965"
     CDS             936..1814
                     /locus_tag="VFU72_09965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5423853.1"
                     /translation="MADHRDPTAAAHATATGHALSDATWLDNHFARSRGIYEAMARAV
                     GLEPGWRVLDAGCGSGSFLPTLAALVGPTGALTALDYDAENVATAAHRAAAAPLACQV
                     SFRQGSVTALPFPDGSFDAVWCANVSQYLTDAELMAALAECRRVVRPDGLVAFKESDG
                     LSDTFWPMDPAVHHRLYDACVRGNLMEGPGVVRAAALRRWLERAGLADVWQRLFPEEY
                     WAPLSPVERVGLGGVLRYLAVGSEEADIPQADRAFWRAQLDPAQPDALVNHPEFYKRG
                     GYVLAVGRVPAAPATA"
     gene            complement(2088..3746)
                     /gene="polX"
                     /locus_tag="VFU72_09970"
     CDS             complement(2088..3746)
                     /gene="polX"
                     /locus_tag="VFU72_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU5423854.1"
                     /translation="MDTSTIVRILTSYADLLEIAGENVFRAQAYRRAADALEALGEPL
                     ESADEGFLLTLPGVGPGTAAAIREIIDTGRLAALDELLQRVPESLLTLLDISGVGAKT
                     AGRLYRELGVTSLVELRQAAESGRVRALKGFGAKQEQRILDGIAFLQSRDDRLNIGEA
                     LPTAELIAALLSERLDTRVAIAGSVRRGKETVGDLNLVALAQDATRVGVALAALPELA
                     QVEPSAPDLLQATLPNGVPVRLALTGPDRFGTALVRWTGSRAHVEQLVARFGELPTAP
                     DEESLYASLGLSWIPPELRQGQDEIELAAEHRLPNLITVQDLRGDLHLHSNWSDGHAT
                     IEQLAEAAVARGYQYLSISDHSDALVVANGLSIERLRAQWRLIDEVNQRVPGVRLLRA
                     AEVEVRRDGSLDYPDQVLAQLDLVVASLHSGLRASREELTRRIVRVLCNPHVDIVAHP
                     TGRIVGHRPEAEYDWDEVFRVARETGTALEINANPSRQDLKDTLARAANDAGVRLAID
                     SDAHNLPSLDLVSYGVGLARRAGIETHAVVNAQPLPDLLTWLQR"
     gene            complement(3783..4880)
                     /locus_tag="VFU72_09975"
     CDS             complement(3783..4880)
                     /locus_tag="VFU72_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5423855.1"
                     /translation="MAVQRFQVGTAESVPGAYAYGVVPVLDLPTGGQETIPVIIAQGR
                     EDGPVLWVTANIHGGELTGIASIHRLLAQNDLPRQMRGTAIFLPTINPAGLRVMQRYP
                     YYEQRDPNRVWPAPPSGEAPSTRPPGIYEAIASVVYELIVGSNPTALLDLHNASIDSI
                     PFTIRDRVLYHDEDQRREAEELARKLDELAKAFGLSLVNESAAAKYVDLQLHRSVAGS
                     VVNTARVPALTLELGMGMAIHPPAVSAGTRGIANVMRYLGMLDGQPRPVDEVPVVDLG
                     FPVRRDDSARAPVSGVVEMLIEPGQSFTEGDLLGRMLDIYGRPLEGGEIPASADGWLI
                     GWSNGIAKYRGQVVASLAVRDQDPLIDAYPQ"
BASE COUNT          717 a         1706 c         1791 g          748 t
ORIGIN      
        1 gccggccaag acgctcttga cctgggagaa ggagctgctc ggctgctacc tcagctcgca
       61 cccgctcaac gactacctgg cgcagatccg ccagcgcggg atggtgcagc tcgccgagat
      121 cgacgaggag tcggtggggc agacggtgga gctgatcgcg ctggtggcag ggctgcgcaa
      181 gctgaccacc aagaccaatc gcaccatggc ggtggtcgag ctggaggatc agagcggctc
      241 gatcgaggcg gtcttcttcc ccgagaccta cgacgcggcg gccgagctgt tggagctgga
      301 cgccatcgtg cacctctccg cccgcgccga tcagcgcaac gatcgcatcc aactggtcgc
      361 caaccggctg acgcgggccg cgctggaccg gcgcgtgccg cagctcgtac gcgaggttca
      421 cctgcgcttg ccgcgcggca cgagccgggc cgagctgaat aagatgcacc ggctgcgcac
      481 cctgctcgac gagtttccgg gcgatgacca ggtggtgctg cacctcccgg cgcgcggcgc
      541 cgaggtggcg ctgctggcgg ggctgcgcat cgactggtgc ggcgatctct ccgcggcgct
      601 tggtgagttg cttggtgaag agcatgtcgg cgtggtggat cgcgaggagt tggtgccggc
      661 cgcggcgctc gcggactgag aggccgtccc gcctggcccg cgcggagcct ctcccaatgg
      721 tgggcgaggg gcgatacaac gagcagcggt agaacgggtt tgaatcccgc cctgctcgtc
      781 gagggccgcg tgaggcacgg acacacctcg ccacatgatc ccatacacat agttgcacct
      841 ggcccggcaa gcgcgcggcc acgccggcat ctgatcgcct gcacgagtgt atgctgtccc
      901 tctggcgcgg atggacaagc gagaggagcg gggcgatggc ggaccacagg gacccgacgg
      961 cggcggctca tgccacagcg accgggcacg cgctctccga cgcgacgtgg ctggacaacc
     1021 acttcgcgcg cagccggggc atctacgagg cgatggcccg cgcggtcggg ctggagccgg
     1081 gctggcgggt gctcgacgcg ggctgcggct ccggctcttt cctgccgacg ctcgccgcgc
     1141 tcgtcggccc caccggcgcg ctgaccgcgc tcgactacga cgccgagaac gtcgccaccg
     1201 ccgcgcaccg ggctgcggcc gcgccgctcg cgtgccaggt gtccttccgg caggggtccg
     1261 tcaccgcgct cccctttccg gacggctcgt tcgacgccgt ctggtgcgcc aacgtctcgc
     1321 agtacctcac cgacgcggaa ctcatggcgg cgctggcgga gtgccgccgc gtggtgcgcc
     1381 ccgacggcct ggtggccttc aaggagtcgg acgggctgtc cgataccttc tggccgatgg
     1441 acccggcggt gcaccaccgc ctctacgacg cctgcgtgcg tggcaacctg atggaaggac
     1501 ccggcgtggt ccgcgccgcc gcgctgcggc gctggctgga gcgcgcgggg ctcgcggacg
     1561 tgtggcagcg gcttttcccg gaggagtatt gggcgccgct ctcccccgtt gagcgggtgg
     1621 ggctcggggg cgtcctgcgc tacctggcgg tggggtccga ggaggccgac atcccccaag
     1681 ccgaccgcgc cttctggcgg gcgcagttgg acccggcgca gcccgatgcc ctcgtcaatc
     1741 accccgagtt ctacaagcgt ggtggctacg tcctcgcggt cggccgcgtg cccgccgcgc
     1801 cagcgacggc gtagcgcggg cacaccacct gtgcgtcgca ccgggtgccg gcgggtgcgc
     1861 cgcacttgcc gggcgaggtg cagatatgtg taccggatca tgtggcgagg tctgcgcggt
     1921 tccctcggct gcgctcggga tgccagagtg aaggcggggc ggacaacgac gaccggtgtc
     1981 gtccgatggg tctctttctt ccccgtcagc ctgagcttgg cgaaggatcc atcgtggagc
     2041 ggcgcccaac gtattcgaac gtacctctgc gcgatgcccg cgcggcctta ccgctgcagc
     2101 caggtgagga ggtcggggag cggctgggcg ttgacgacgg cgtgcgtctc gatgccggcg
     2161 cggcgggcca ggccgacgcc gtagctgacc aggtcgagcg acgggaggtt gtgcgcgtcg
     2221 gagtcgatcg ccaggcgcac gcccgcatca ttggcggcgc gagccagcgt gtccttgaga
     2281 tcctggcggg aggggttggc gttgatctcg agggccgtac cggtctcgcg tgccacgcgg
     2341 aagacctcgt cccagtcgta ctcggcctcg gggcggtggc cgacgatcct tccggtcggg
     2401 tgggccacga tgtcgacgtg cgggttgcac agcacccgca cgatgcgccg cgtcagctct
     2461 tcgcgcgagg cacgcagccc ggagtggagc gaggcgacca ccaggtcgag ttgagcaagc
     2521 acctggtcgg ggtagtcgag cgagccgtcg cggcgtacct cgacctcggc ggcccgcagc
     2581 agccgcacgc cgggcacccg ctggttgacc tcgtcgatga gccgccactg ggcccgcagc
     2641 cgctcgatcg agagcccgtt ggcgaccacc agcgcgtcgg agtggtccga gatgctgaga
     2701 tactggtaac cccgcgccac cgccgcttcg gccagttgct cgatggtggc gtggccgtcg
     2761 ctccaattgg agtggagatg cagatccccg cgcaggtcct gcacggtgat caggttgggc
     2821 aggcggtgct ccgcggccag ctcgatctcg tcctggccct gccgcagctc cggcggaatc
     2881 caggagagcc ccagggaggc gtagagactc tcctcatccg gcgcggtggg cagctcccca
     2941 aagcgcgcga ccaattgctc gacatgcgcg cggctgccgg tccaccgcac cagcgcggtg
     3001 ccgaagcggt ccggcccggt cagcgccagg cgcaccggca cgccgttcgg cagcgtcgcc
     3061 tgcagcagat cgggcgcgga cggctctacc tgagccagtt caggaagcgc ggccagcgcc
     3121 acgccgaccc gcgtcgcgtc ctgtgccagg gcgacgagat tgagatcgcc gaccgtctcc
     3181 ttgccgcggc gcacgctgcc ggcgatcgcc acgcgcgtgt ccagccgctc gctcagcagc
     3241 gcggcgatca gctcggccgt gggtagcgcc tcgccgatgt tgaggcggtc gtcgcgcgac
     3301 tggaggaagg cgatgccgtc cagaatgcgc tgctcctgct tggcgccgaa gcccttgagc
     3361 gcgcgcaccc tgccgctctc ggcggcctgg cgcagctcga ccaggctcgt cacgcccagt
     3421 tcgcggtaga gcctcccggc cgtcttggcc cccacgccgg agatgtcgag cagggtcagc
     3481 aacgactccg gcacccgctg cagcagctcg tcgagcgccg ccagccgacc ggtgtcgatg
     3541 atctcgcgga tggcggcggc ggtgccgggg ccgacgccgg gcagcgtcag caggaagcct
     3601 tcgtcggcgc tctccagcgg ctcgccgagc gcttccagcg catcggccgc ccggcgatag
     3661 gcctgcgcgc ggaacacgtt ctcacccgcg atctccagga gatcggcgta gctggtgagg
     3721 atgcggacaa tcgtgcttgt atccaccgtt ggctcacgac gggcgccgcc cgcggcgccc
     3781 ggctattgag gataggcgtc gatcagcgga tcctgatcgc gcacggccag tgaggcgacg
     3841 acctgccccc ggtacttggc gatcccgttg gaccagccga tcaaccagcc atcggcgctc
     3901 gcaggaatct cgccaccctc cagcgggcgg ccatagatgt cgagcatccg gcccagcagg
     3961 tcgccctcgg taaacgattg gcccggctcg atcagcatct cgaccacgcc cgagaccggc
     4021 gcgcgcgccg agtcgtcgcg gcgcaccggg aagccgaggt cgaccactgg aacctcatcg
     4081 accggccgcg gctgaccgtc gagcatgccg aggtaacgca tcacgttggc gatgccgcgc
     4141 gtcccggccg acaccgccgg cgggtggatc gccatgccca tgcccagctc cagcgtcagc
     4201 gccggcacgc gcgccgtgtt gaccacgctg ccggctaccg agcggtgcag ctgcaggtcg
     4261 acgtacttag cggcggcgga ctcgttgacc agcgacaggc caaaggcttt ggccaactcg
     4321 tcgagcttgc gtgcgagctc ctccgcctca cggcgctgat cttcatcgtg gtagagaacg
     4381 cggtcgcgga tggtgaacgg aatcgagtcg atcgaggcgt tgtgcaggtc gaggagggcg
     4441 gtcgggttgc tgccgacgat caactcatag acgaccgacg cgatcgcctc atagatgccg
     4501 ggtgggcggg tggacggtgc ctcgccgctc gggggagcgg gccagacacg gttggggtcg
     4561 cgctgctcgt agtaggggta acgctgcatc acgcgcagac cggcgggatt gatcgtcggc
     4621 aggaagatgg cggtaccgcg catctgccgg ggcagatcgt tctgggccag caaccggtgg
     4681 atgctggcga tcccggttag ctcgccgccg tggatgttgg ccgtgaccca gaggaccggc
     4741 ccgtcctcgc gcccctgggc gatgatgacc gggatggtct cctgcccgcc ggtgggcaga
     4801 tcgagcacgg gaacgacgcc gtaggcgtag gcaccgggaa cgctctccgc cgtcccgacc
     4861 tggaagcgtt gcaccgccat gcgctgatct cctgacctca tccgcgaatc agagcccggc
     4921 agggtgccgg acacccgatc ttacgtcagg agcgccgtgc gg
//
LOCUS       DASUBI010000517         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1042228, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000517 DASUBI010000000
VERSION     DASUBI010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042228"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFU72_09980"
     CDS             <1..704
                     /locus_tag="VFU72_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5423856.1"
                     /translation="VFVIIGIRALASQIPQVAQQFNYGTLYNVLSTILLIFAAATAPE
                     MLADDRRQNVLPLYFSRPISRADYVLAKVGALGILMGTISLVPALLLFVADTLLASDP
                     ASYLLHHLGLLLRVLATGSLLALYFAAIGLVIASFTDRKAVAAAVYIGAMLVLSGIAA
                     ALFHTIGPDFRDYVALVDPVAVPLGLAGWIIGFRSGHDTLANSALGGGWFLADLLVIS
                     AVAAGVLYRRYLRHA"
     gene            697..1665
                     /locus_tag="VFU72_09985"
     CDS             697..1665
                     /locus_tag="VFU72_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5423857.1"
                     /translation="MLEARPTREQSAAPPGARQPTIVVEHLSKWYGDVVAVSDVSFHV
                     EPGVTGLLGPNGAGKSTILKTLSGLLAPSTGSVRVLGQPVRGNPAIYRQIGLVPEQEG
                     VYPFLTGREFVRLNAVMQHLDDPEGAVRAALALVELERDADRAVRGYSKGMRQRIKIA
                     GGLVHDPEVLILDEPLTGTDPVQRLRLMELIRRLGDEGKTVLVSSHVLHEVERVADRI
                     LVLVDGKLAAAGDYRAIRDQIDEHARMVRVRAGDIRGLLADLVGQEWLETVRFEGDGA
                     AVLETNDVRALYRALPAVARRLRIRLYEITALDESLSSVFAYVTER"
     gene            1666..2412
                     /locus_tag="VFU72_09990"
     CDS             1666..2412
                     /locus_tag="VFU72_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5423858.1"
                     /translation="MSQLMRAAPVTWLTVREFGAGRAIRVTALFAATPIVFALIYLAQ
                     SNRPSGVNFLGDLFLGFLATIVIPLATLILATAALGNELSDRTIPYLALKPVGRWRIV
                     LEKFAGTVLVTAVPLLLGIVITWGIVASQEASAGGRVLLAMLAATLAGILAYGALFLF
                     ISLVIPRALIVGIIYVLLWEGLIVALISGARVLSVRHYINSIFVHVLDNRAVRVDQVA
                     SLATALVVLALVVVVSLALASRRLSRMSLD"
     gene            2530..3618
                     /locus_tag="VFU72_09995"
     CDS             2530..3618
                     /locus_tag="VFU72_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5423859.1"
                     /translation="MDPQEPAIAAYQAALDDYLQTREEQALFRASLLSQHFVESGLGP
                     DDIIALHFEALEYGLSRRTPRQQARAVTDAHQFLLEVMIAYGVKYKEYLELKLRDSLR
                     EAEARAELERQRALEAERVEREKAELLAGIAHELRTPITAAKGNLDLAVRRLSSGQAD
                     TLAPLLGSTREALDRLSRLTADLVQVSRGETPLLERAPQDITELLAQACSWARPTAAT
                     KGVDLILETQQPIVLRCNPDALLSVFGNLLSNAVRYTPPGGSVHVRHELREGEIWVEV
                     RDTGIGISPEVQARIFEKFYRGDDARRLDGQGLGLGLTLVQQMVQAHRGRIELESEPA
                     RGSAFRVILPREMEPEQEGERAHVAGAE"
     gene            3599..>3991
                     /locus_tag="VFU72_10000"
     CDS             3599..>3991
                     /locus_tag="VFU72_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5423860.1"
                     /translation="MSQEPSEPTRQSAGQTRLIAEQRQTISELQTPVIEVWEGILALP
                     IIGSVDTARAQQMTEALLQKIVDASAEIVILDITAVPVVDTAVARHLLETVSAARLLG
                     AEVMVVGLSARTAMTLVHLGVELSGVTTR"
BASE COUNT          551 a         1374 c         1411 g          655 t
ORIGIN      
        1 cggtcttcgt gatcatcggc atccgcgccc tggcctcgca gatcccccag gtggcgcagc
       61 agttcaacta cggcacgctc tacaacgtgc tctccaccat cctgctgatc ttcgccgccg
      121 ccaccgcgcc cgagatgctg gccgacgacc ggcgccagaa cgtcttgccg ctctacttct
      181 cgcgcccgat cagccgggcc gactacgtgc tggccaaggt cggtgcgctc ggcatcctga
      241 tgggcacgat ctcactcgtg ccggcgctgc tgctgttcgt cgccgacacg ctgctggcct
      301 ccgaccccgc cagctatctg ctgcaccacc tcggcctgct gctgcgcgtg ctggcgaccg
      361 gctcgctgct ggcgctctac ttcgcggcca ttgggctggt gatcgcgtcc ttcaccgacc
      421 gcaaagcggt ggcggccgcg gtctacatcg gcgccatgct cgtcctctcc ggcatcgcgg
      481 cggcgctctt ccacacgatc ggcccggatt tccgcgacta cgtggcgctg gtcgatccgg
      541 tggcggttcc gctggggctg gccggctgga tcatcggctt ccgctccggc cacgacacgc
      601 tggccaattc cgcgctcggt ggcggctggt tcctggctga tctgctggtc atcagcgccg
      661 tcgccgcggg tgtgctctac cggaggtacc tgcgccatgc ttgaggcacg cccgacgcgg
      721 gagcagtccg ccgcgccgcc gggcgcgcgc cagcccacca tcgtggtcga gcacctctcc
      781 aagtggtacg gcgacgtggt ggcggtctcc gatgtctcgt ttcacgtcga gccgggtgtg
      841 accgggctgc tggggccgaa tggcgccggc aagtcgacca ttctcaagac gctcagcggc
      901 ctgctggcgc cctcgaccgg gagcgtgcgc gtgcttggcc agccggtgcg cggcaacccc
      961 gccatctatc gccagatcgg gctggtgccg gagcaggagg gcgtctaccc cttcctgacc
     1021 gggcgcgagt tcgtgcggct caacgccgtc atgcagcacc tcgacgaccc ggagggcgcc
     1081 gtgcgcgcgg cgctggcgct ggtcgagctg gagcgcgacg ccgaccgggc cgtgcgcggc
     1141 tactccaagg ggatgcggca gcgcatcaag atcgcggggg ggctggtgca cgaccccgag
     1201 gtgctgatcc tcgacgagcc gctgacgggc accgacccgg tgcagcgcct gcgcctgatg
     1261 gagctgatcc gccggctcgg cgacgagggg aagacggtgc tggtctcctc ccacgtgctg
     1321 cacgaggtcg agcgcgtggc cgaccgcatc ctggtgctgg tggacggcaa gctggccgcc
     1381 gccggggact accgcgccat ccgcgaccag atcgacgagc acgcccgcat ggtgcgggtg
     1441 cgcgccggcg atatccgtgg cctgctggcc gatctggtgg ggcaggagtg gctggagacg
     1501 gtgcgcttcg agggtgacgg cgccgcggta ctcgagacca acgacgtgcg cgcgctctac
     1561 cgcgcgctgc cggccgtcgc ccggcggctg cggatccggc tctacgagat cacggcgctc
     1621 gacgagtcgc tgtcgagcgt cttcgcctac gtgacggagc gctaattgtc ccagctcatg
     1681 cgcgcggccc cggtgacctg gctgacggtg cgcgagttcg gcgccggccg ggcgatccgc
     1741 gtcaccgcgc tcttcgccgc caccccgatc gtcttcgccc tgatctatct ggcgcagtcg
     1801 aaccgcccca gcggggtcaa tttcctgggt gacctctttc tgggcttcct ggcgaccatc
     1861 gtcatcccac tggcgacgct gatcctggcg accgccgcgc tgggcaacga gctctccgat
     1921 cgcaccatcc cctacctggc gctcaagccg gtcggccgct ggcgcatcgt gctggagaag
     1981 ttcgccggca ccgtgctggt gacggcggtc ccgctgctct tgggcatcgt catcacctgg
     2041 ggcatcgtcg cctcgcagga ggcgagcgcg ggagggcggg tgctgctggc gatgctggcg
     2101 gccacgctgg ccggcatcct ggcctacggc gcgctcttcc tgttcatcag cctggtcatc
     2161 ccgcgcgcgc tgatcgtcgg gatcatctac gtgctcctct gggaggggct gatcgtcgcg
     2221 ctgatctcgg gcgcgcgggt gctgagcgtg cggcactaca tcaactcgat cttcgtgcat
     2281 gtgctcgaca accgggcggt gcgcgtggac caggtggcgt cgctcgccac ggcgctggtg
     2341 gtgctcgcgc tggtcgtggt cgtctcgctg gcgctggcca gccgccgcct gagccgcatg
     2401 agtctcgact gagcatgact tgcgcccgcg tgaccgttgc tgaaaggtca atcgcgaagt
     2461 agcattgcct cccggaggaa tccgtcgtca gagcacaggg gagcgcaact cgctatcggg
     2521 gggtggccaa tggacccgca ggagcccgcg atcgcggcct atcaggcggc gctcgacgac
     2581 tatctgcaga ctcgcgagga gcaggcgctc tttcgagctt cgctcctgag ccagcacttc
     2641 gtcgagagcg gactggggcc cgacgacatc atcgcgctcc acttcgaagc gctggagtac
     2701 gggctgtccc ggcgcacgcc gcgccagcag gcccgcgccg tgaccgacgc gcatcagttc
     2761 ctgctcgaag tcatgattgc ctacggcgtg aaatacaagg aatacctgga gctgaagctg
     2821 cgcgacagcc tgcgcgaggc cgaggcccgg gccgagctgg agcggcagcg cgcgctggag
     2881 gccgagcgcg tcgagcgcga gaaggcagag ttgctggcgg gcatcgctca cgagctgcgc
     2941 accccgatca cggccgccaa ggggaatctc gatttggcgg tacgccggct ctcgagtggg
     3001 caggccgata cgctggcgcc gctgctgggg tcgacgcgcg aggcgctcga ccgtctctcg
     3061 cgcctgaccg ccgatctggt gcaggtcagc cgtggggaga cgccgctatt ggagcgcgcc
     3121 ccgcaggata ttaccgagct cctcgcgcag gcctgctcct gggctcggcc aaccgcggcc
     3181 accaagggtg tcgacctgat cctggagacg cagcagccga tcgtgctgcg gtgcaacccc
     3241 gacgcgctgc tcagcgtctt cggcaatctc ctctcgaacg cggtgcgcta cacgccgcct
     3301 ggcgggtcgg tgcacgtccg ccacgagctg cgcgagggcg aaatctgggt cgaggtgcgc
     3361 gacaccggca tcggcatctc gcccgaggtg caggcgcgca tcttcgagaa gttctatcgg
     3421 ggggacgacg cccgtcgcct ggacggccag gggctggggc tggggttgac cctggtgcag
     3481 cagatggtac aggcgcaccg gggccgcatc gagctggaga gcgagccggc gcgcggcagc
     3541 gccttccgcg tcatcctgcc gcgggagatg gaacctgagc aggagggaga acgcgcccat
     3601 gtcgcaggag ccgagtgagc ccacccgcca gagcgccggg caaacgcgcc tcatcgcgga
     3661 gcagcggcag acgatcagcg agctgcagac gccggtgatc gaggtctggg agggcatact
     3721 ggcgctgccg atcatcggca gcgtcgacac ggcgcgggcg cagcagatga ccgaggcgct
     3781 gttgcagaag atcgtcgatg ccagcgctga gatcgtgatc ctcgacatca cggcggtgcc
     3841 ggtggtcgac accgccgtgg cccgacatct gctggagacg gtctccgccg cgcggctgct
     3901 gggcgcggag gtcatggtcg tggggctctc ggcccgcacc gccatgacgc tggtccacct
     3961 gggtgttgag ctcagcggtg tgaccacgcg g
//
LOCUS       DASUBI010000518         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_865405, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000518 DASUBI010000000
VERSION     DASUBI010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865405"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1579)
                     /locus_tag="VFU72_10005"
     CDS             complement(26..1579)
                     /locus_tag="VFU72_10005"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU5423861.1"
                     /translation="MTTTEESRRAELEALQRALAAQEAESAARQHQRGKLSARERVEQ
                     LVDPGSFVELDALVRHRSTYFGMAERRPYGDGVVTGYGTIDGRTVALFSQDFTVFGGS
                     LGEAFAEKMVKIMDLALKVGCPIIGINDSAGARIQEGVEGLNGYGEVFYRNVQASGVV
                     PQLSIIAGPCAGGAVYSPAMTDFTLMVKGISQMFITGPDVIKTVTGEEVSHEQLGGAM
                     THTSISGVAHLAAEDEADLFELVRGLLSFLPSNNLEEPPHFPTGDDPLRADPALDTLV
                     PEVSTRPYDMHEVIRHVVDDGEFLEIQPAWARNIIIGFARLDGHAIGVVANQPNVLAG
                     TLDIDASTKAARFVRFCDAFNIPLVTFEDVPGFLPGTNQEYGGIIRHGSKLLFAYCEA
                     TVPKLTVITRKAYGGAYVVMCSRSIGADFVAAWPTAEVAVMGPSAAVNLIFRREIAGA
                     DDAEAKRRELVADYEREFANPFQVASRGYVDDVILPRDTRRWLIRALAVARNKRLAAP
                     ARKHGNIPL"
     gene            complement(1613..>2932)
                     /locus_tag="VFU72_10010"
     CDS             complement(1613..>2932)
                     /locus_tag="VFU72_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5423862.1"
                     /translation="PTQLGYDSDAPEARAEVGRTGVAISTVEDMRRLFEGIDLGEVTT
                     SMTINAPAALLLLMYQIVAEEQGVEVARLGGTIQNDILKEYAARGTYIFPPRPSMRLV
                     TDTFAYCGRVLPHWNVISISGYHIREAGSTAVQEVAFTLANAVAYVQAALAAGLDIDD
                     FAPRLSFFFNAHNNLFEEVAKFRAARRMWAKLTRERFGAHDPKSWMLRFHTQTAGSSL
                     TAQQPLNNVVRVAYQALAAVLGGTQSLHTNGYDEALALPTEEAATLALRTQQILSDET
                     GVPETADPLGGSWFVEALTDELEQRAWALLDEIAERGGAVAAIESGFMQEQIAESAYR
                     FELQLAAHERALVGVNEYRDDEDEITVPLHHIDDEAVRGQIERVAAYKARQNRARVEQ
                     ALAGVEAAARGTENLLPLMKAALVTGATLGEICGRLRGAWGEYRPAS"
BASE COUNT          432 a         1051 c          977 g          472 t
ORIGIN      
        1 gcgaagcacg actcgtcgcc ggcgctcaca gcgggatgtt gccgtgcttg cgcgccgggg
       61 cggccaggcg cttattgcgc gccactgcca gcgcccgaat cagccagcgg cgcgtgtcgc
      121 gcggcaggat cacgtcgtcg acgtagccgc gcgaggcgac ctggaacggg ttggcaaact
      181 cgcgctcgta gtcggccacc agctcacggc gcttcgcctc ggcatcatcg gcgcccgcga
      241 tctcgcgccg gaagatcagg tttaccgccg ccgatggccc catcaccgcc acctcggcgg
      301 tcggccaggc ggccacgaag tcggccccga tcgagcgcga gcacatcacg acatacgcgc
      361 cgccgtaggc cttgcgcgtg atcacggtca gcttgggcac cgtggcttca cagtaggcga
      421 agagcagctt ggagccgtgg cggatgatcc cgccgtactc ctggttggtg ccgggcagga
      481 agccgggcac gtcctcgaag gtcaccagcg ggatgttgaa ggcgtcgcag aagcgcacga
      541 agcgcgccgc cttggtcgag gcgtcgatgt cgagcgtgcc ggccagcacg ttcggctgat
      601 tggccaccac cccgatggcg tggccgtcga gtcgggcgaa gccgatgatg atgttgcgcg
      661 cccaggccgg ctggatctcc aggaactctc cgtcgtcgac cacatgccgg atgacctcgt
      721 gcatgtcgta gggccgggta ctgacctccg gcaccagcgt gtcgagcgcc ggatcggcgc
      781 gcagcgggtc gtcgccggtc gggaaatgcg gcggctcctc caggttattc gagggcagga
      841 agctgagcag tcctcgcacc agctcgaaga gatcggcttc gtcctcggcc gccagatgcg
      901 ccaccccact gattgaggta tgggtcatcg ccccgccgag ctgctcgtgg ctcacctcct
      961 cgcccgtgac cgtcttgatc acgtccgggc cggtgatgaa catctggctg atccccttga
     1021 ccatgagggt gaagtcggtc atggccggcg agtagaccgc gccgccagcg cagggccccg
     1081 cgatgatcga gagctgcggc accacgccgg aggcctgcac gttgcggtag aagacctcgc
     1141 cgtagccgtt cagcccctcg acgccctcct ggatgcgcgc gcccgccgaa tcgttgatgc
     1201 cgatgatcgg gcagcccacc ttgagcgcca gatccatgat cttgaccatc ttctcggcga
     1261 acgcctcgcc caggctgccg ccgaagacgg tgaaatcctg tgagaagagc gccacggtgc
     1321 gtccgtcgat cgtgccgtag cccgtgacga cgccgtcgcc gtagggccga cgctcagcca
     1381 tgccaaagta ggtcgagcgg tggcgtacca gcgcgtccag ctcgacgaaa gagccggggt
     1441 cgaccagttg ctccacgcgc tcgcgcgccg agagcttgcc gcgctggtgc tgcctcgccg
     1501 cgctctcggc ctcctgggcc gccagcgccc gttgcagcgc ctccagctcc gcccgcctgg
     1561 attcctcggt cgtcgtcacc gcgccatccc cgtcatcacg cccacggctg cctcaactcg
     1621 ccgggcggta ctcgccccac gccccgcgca gcctgccgca gatctcgccc agcgtcgcac
     1681 ccgtcacgag cgccgccttc atcagcggca gcagattctc cgtaccccgg gcagcagcct
     1741 ccacccccgc cagcgcctgc tccacccgcg cgcggttctg gcgcgccttg taggccgcca
     1801 cgcgctcgat ctggccccgc accgcctcgt cgtcgatgtg gtggagcggc accgtgatct
     1861 cgtcctcatc gtcgcggtac tcgttcacgc ccaccagcgc gcgctcgtgc gcggccagtt
     1921 gcagctcgaa acggtaggcg ctctcggcga tctgctcctg catgaagccg ctctcgatcg
     1981 ccgccacggc gccgccacgc tcggcgatct cgtccagcag cgcccaggcg cgctgctcca
     2041 gctcgtcggt cagcgcctcg acgaaccagg agccgcccag aggatcggcg gtctccggca
     2101 ccccggtctc gtcgctcagg atctgctgcg tgcgcagggc cagcgtggct gcctcctcgg
     2161 tcggcagcgc cagcgcctca tcatacccgt tggtgtgcag gctttgggtg ccgccaagca
     2221 ccgccgccag cgcctgatag gccacccgta ccacgttgtt cagcggctgc tgcgcggtga
     2281 gcgagctgcc ggccgtctgg gtgtggaagc gcagcatcca cgacttgggg tcgtgggcgc
     2341 cgaaccgctc gcgtgtgagc ttggcccaca tgcgacgcgc ggcgcggaac ttggcgacct
     2401 cctcgaagag gttattgtgc gcgttgaaga agaagctgag gcgcggggcg aagtcgtcga
     2461 tgtcgagccc ggcggccagc gccgcctgga cgtaggcgac cgcgttggcc agcgtgaagg
     2521 cgacctcctg caccgcggtc gagccggcct cgcggatatg gtagccgctg atgctgatca
     2581 cgttccagtg gggcagcacg cggccgcagt aggcgaaggt atcggtcacc agccgcatcg
     2641 aagggcgcgg tgggaagatg taggtgccgc gcgccgcgta ctctttgagg atgtcgttct
     2701 ggatcgtgcc gcccagccgc gccacctcga ccccctgctc ttcggccacg atctgataca
     2761 tcaggagcag cagcgccgcg ggcgcgttga tcgtcatgga ggtcgtaacc tcgcccaggt
     2821 cgatcccctc gaacaggcgg cgcatgtctt caaccgtgct gatggcgacg ccggtgcggc
     2881 cgacctccgc acgcgcctcc ggcgcgtccg agtcgtagcc gagctgggtc gg
//
LOCUS       DASUBI010000519         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_750322, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000519 DASUBI010000000
VERSION     DASUBI010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750322"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..669)
                     /locus_tag="VFU72_10015"
     CDS             complement(154..669)
                     /locus_tag="VFU72_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEU5423863.1"
                     /translation="MERVVPAVDWRPPEPLRGGAEMAALRPFHFDCRWRGQVAAGGMG
                     PGSPVMDAVGQGIFRPILDGAWLAGDFEQDQFVAGERLLTWKAHYVVGWDPRVGEYHV
                     TYVDNAGNASVLRGRIEGPRFIIESLAGEPVRLRMSWDLLEPGRVAWRNECSVAGGPW
                     QLIEEYLCTPL"
     gene            complement(890..2107)
                     /locus_tag="VFU72_10020"
     CDS             complement(890..2107)
                     /locus_tag="VFU72_10020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5423864.1"
                     /translation="MAERAEVAIVGGGIMGTSLAFALTRLGVRDVLLLERGTLAAGAS
                     GKTGALLRQHYTNVPEATLAHRSLQIFAHWADVVGGDCGFAPAGSIITVATRGADAVN
                     VARMRRNVALLNSLGVKAEVIAPDRLRELQPFTVAEDIDAAVYEPESGYVDAVAATRG
                     MAEAALRGGARLRERCAVTGLRAQDGRVVGLETEAGPVEAGTVVVAGGAWSLPLLAAL
                     GVEVPLQAQRVQVVVLNRPLAMPESGTMCYVDTAAGFFCRDAGPNRTLAGVGGGEFHD
                     VVDPFAYDERLNPGFGETVKRFMARRMPAMAEASTLYGYACLYDLSPDAHPILGGVEG
                     YDNLHLMLGFSGAGFKKGPATGQALAELIVHGKASFVDLTPFRLSRFQDEAWRAPWSP
                     DEYSFSTDFGHKF"
     gene            2331..>2928
                     /locus_tag="VFU72_10025"
     CDS             2331..>2928
                     /locus_tag="VFU72_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5423865.1"
                     /translation="MSDELRQITREFVLSPEFQQAVAAAEAEPYAVPRDFLRRAVELG
                     ALGMDIPEEYGGSGLSPTEQVAVLEELARGHAGLALAVLVQNSLTAFPIAQFGSEEQK
                     RRYLPRMASGELLVSFGLSEPKNGSDAKGIQLRATRDEVSGGWRLNGTKRWITNADRA
                     GLFVLAARTGTPESRGHGLTTFLVEVSPETEGFLMPKPY"
BASE COUNT          460 a         1092 c          994 g          382 t
ORIGIN      
        1 ccaccgcgcc cctgcggacg acaggcgaat ctcaacgact cgcgcgccta ccagcaggaa
       61 taccgccacc gccccggatc gcgccgcggt cggggcaatc ggccgggatt cggcgccgtg
      121 gaacatggtc accacgtagc attgacgcgc gggctacagc ggtgtgcaga gatactcctc
      181 gatgagctgc caggggccgc cggcgacgga gcactcgttg cgccaggcca cgcggcccgg
      241 ctccaggaga tcccagctca tgcgcagccg caccggctcc ccggcgaggc tctcgatgat
      301 gaagcgcggc ccctcgatcc gcccgcgcag tacgctggcg ttgccggcgt tgtcgacgta
      361 ggtgacgtgg tactcgccca cgcgcgggtc ccagccgacg acatagtgcg ccttccaggt
      421 gagcaggcgc tcgccggcca cgaattggtc ctgctcgaaa tcgccggcca gccaggcgcc
      481 gtcgaggatc ggcctgaaga tcccctggcc gaccgcgtcc atcacgggcg agccgggccc
      541 catgccgccc gccgcgacct ggccgcgcca gcgacagtcg aagtggaagg ggcgcagcgc
      601 cgccatctcg gcgccgccgc gcagcggctc cggcggccgc cagtcaacag ccggtacgac
      661 tcgttccatg cccctcgcct cctcgtgggc ctctggccag cagactcgga atcaggatag
      721 ccacgacggc gtctcggagc cgtcacggga cacgaacgag cgttacagca gcgtgacaaa
      781 gagatcacta tgagctgaaa cacgctgagc ggagccaaag gtggagtcgg ctgagaaata
      841 accgggcgct gcgtcctcca gcagacccgg tgccgccacc ggcacgcgct cagaacttgt
      901 ggccgaagtc ggtggagaag ctgtactcgt cggggctcca gggcgcgcgc caggcttcat
      961 cctggaagcg gctgaggcgg aagggggtga gatcgacgaa ggaggctttg ccgtgcacga
     1021 tcagctcggc cagggcctgg ccggtggccg ggcccttctt gaagcccgcg ccgctgaagc
     1081 cgagcatgag gtgcaggttg tcgtagccct cgacgccgcc caggatcggg tgggcgtcgg
     1141 ggctgaggtc gtagaggcag gcgtagccgt agagcgtgct cgcctcggcc atggccggca
     1201 tgcgccgcgc catgaagcgc ttgaccgtct caccgaagcc ggggttcagg cgctcgtcgt
     1261 aggcgaacgg atcgactaca tcgtggaact cgccgccgcc gacgccggcc agcgtgcggt
     1321 tcggcccggc gtcgcggcag aagaagccgg cggcggtgtc gacgtagcac atggtgccgc
     1381 tctcgggcat ggccagcggc cggttgagca cgaccacctg tacccgctgc gcctgcagcg
     1441 gcacctccac cccgagcgcc gccagcagcg gcagcgacca ggcgcccccg gccaccacca
     1501 ccgtgccggc ctcgaccggc ccggcctcgg tctccagccc caccacgcgc ccgtcctgcg
     1561 cccgcagccc ggtcacggcg caacgctcgc gcagccgcgc cccgccgcgc agcgccgcct
     1621 cggccatgcc gcgcgtggcc gccaccgcgt cgacgtagcc ggactccggc tcgtagaccg
     1681 ccgcgtcgat gtcctcggcc acggtgaagg gctgtaactc gcgcagccgg tccggcgcga
     1741 tgacctcggc cttgacgccc aggctgttca gcagcgccac gttgcgccgc atgcgcgcga
     1801 cgttgacggc gtcggcgccc ctggtcgcca ccgtgatgat cgagccggcc ggcgcgaagc
     1861 cgcagtcgcc gccgacgacg tcagcccagt gggcgaagat ctgcaggctg cggtgggcca
     1921 gcgtcgcctc gggcacgttc gtatagtgct ggcgcaagag cgcgccggtc ttaccgctcg
     1981 cccccgccgc cagcgtgccc cgctccagca gcagcacatc gcgcacgccc agccgcgtca
     2041 gggcaaacgc caggctcgtc cccatgatcc cgccgccgac gatcgcgacc tctgcccgct
     2101 ccgccatcag caaccccgat tcgtgtccag atacgtactc tcccagagta tcgcacgcgc
     2161 ggcccgcgtg agcgccgctg cccgcttctt gcggacggcg acgcaggacc tacaatcggg
     2221 tccggagttc tcggcgcggg acgctccgcc cgcacaggat cgcgcgacca cgagcggcgc
     2281 gccggttgga tgcaccacca cagcgcggtg gcacagaaag cgagcaggcc atgtcggacg
     2341 agcttcgtca gatcacccgt gaattcgtcc tcagccccga atttcagcag gcggtcgccg
     2401 ccgccgaggc cgagccctac gccgtgccgc gcgacttcct gcggcgcgcc gtcgagctgg
     2461 gcgcactcgg catggacatc cccgaggagt acggcggcag cgggctgagc ccgaccgagc
     2521 aggtggcggt gctggaggag ctggcgcgcg gccacgccgg gctggccctc gccgtgctgg
     2581 tgcagaactc gctgaccgcc ttcccgatcg cccagttcgg cagcgaggag cagaagcgca
     2641 ggtatctgcc gcgcatggcc agcggcgaac tgctcgtctc cttcggcctc agcgagccca
     2701 agaacggctc cgacgccaag ggcattcaac tgcgcgccac ccgcgacgag gtgagcggcg
     2761 gctggcggct gaacggcacc aaacgctgga tcaccaacgc cgaccgcgcc gggctgttcg
     2821 tgctggcggc gcgcaccggc acccccgaga gccgcggcca cggcctcacc accttcctgg
     2881 tcgaggtctc tcccgagacc gagggcttcc tgatgcccaa gccctacg
//
LOCUS       DASUBI010000520         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_886860, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000520 DASUBI010000000
VERSION     DASUBI010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886860"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFU72_10030"
     CDS             <1..394
                     /locus_tag="VFU72_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5423866.1"
                     /translation="EAASRIELADSADVIRRKIRRAVTDSEAEVRYDEATKPAISNLM
                     TIYGLFTGESMAEIERRYAGQGYGAFKGDLAEIVVEGLAPLQRRLEELSAEPQEVVRI
                     LHEGADRARALATVKMERVRAVTGLGLP"
     gene            425..919
                     /locus_tag="VFU72_10035"
     CDS             425..919
                     /locus_tag="VFU72_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5423867.1"
                     /translation="MAVEIGTVAPEFTLRDAQENQEYTLKEALARGPVLLGIYKSSCQ
                     ASKTVFPFLERITQVYPDERLTVWGISQDSANVTRSFARRYEITFPLLVDEHDYQVSR
                     AYDIQATPSLFLLNRDGQIVFQGMGFQKPAMNELSAAVAELLQADAVDITEGSDDVPG
                     WVPG"
     gene            1059..1400
                     /locus_tag="VFU72_10040"
     CDS             1059..1400
                     /locus_tag="VFU72_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423868.1"
                     /translation="MPVERVNWADVPVETVYPGITRQVVQGARQTLVRYVYQPGAVFP
                     RHQHPQEQITAVLSGAIEFEVAGERHRLGAGEVAVIPGDTPHGARVLGDDVVETLNAL
                     SPRRDQAPGPG"
     gene            1492..1749
                     /locus_tag="VFU72_10045"
     CDS             1492..1749
                     /locus_tag="VFU72_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423869.1"
                     /translation="MIAPTRDDLHHFIDQLSDEDLRLAAGVLLTLLAERDPLLAALLV
                     APEDDEPLTPDELAAIERSRAELDRGASVPWEQVRRELLEE"
     gene            1754..2008
                     /locus_tag="VFU72_10050"
     CDS             1754..2008
                     /locus_tag="VFU72_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU5423870.1"
                     /translation="MSWQLVLPESIRTQLRELPREDRQAIGRAIDQLVRDPGQADIRK
                     LRGRPDWRLRVGRWRVIFEMNTRASEIHVLAVDDRKDVYR"
BASE COUNT          361 a          751 c          765 g          312 t
ORIGIN      
        1 cgaggcggcc agccgcatcg agctggccga cagcgccgac gtcatccggc gcaagatccg
       61 ccgcgccgtc accgactccg aggcggaggt gcgctacgac gaggcgacca agccggccat
      121 ctccaacctg atgacgatct acgggctctt caccggcgag tcgatggccg agatcgagcg
      181 gcgctacgcc ggtcagggct acggcgcctt caagggcgac ctggccgaga tcgtggtcga
      241 ggggctggcg cccctccagc ggcggttgga ggagctgtcc gccgaaccac aggaggtcgt
      301 gcgcatcctg cacgaggggg cggaccgggc gcgggccctc gccacggtca agatggagcg
      361 cgtgcgcgcg gtgaccggat tggggctacc gtagcggcga gggtttgaga ggagcggcga
      421 gcgaatggcg gtcgagatcg ggacggtggc gccggagttt accctgcgcg acgcccagga
      481 gaatcaagaa tacacgctga aggaagcgct ggcgcgtggg ccggtgctgc tcggtatcta
      541 caagtcgtcc tgccaggcca gcaagacggt ctttcccttt ctggagcgca tcacgcaggt
      601 ctatcccgat gagcgcctga ccgtgtgggg tatctcgcag gactcggcca atgtcacccg
      661 ctccttcgcg cggcgctacg agatcacctt cccgctgctg gtcgacgagc acgactacca
      721 ggtctcgcgc gcctatgaca tccaggcgac cccctcactc ttcctgctca accgcgacgg
      781 ccagatcgtc ttccagggca tgggcttcca gaagccggcc atgaacgagt tgagcgcggc
      841 ggtggccgag ctgctgcagg ccgacgcggt cgacatcacc gaggggagcg acgacgtccc
      901 cggctgggtg cctggctgac cctccaagca tctgggctag gttctagccc acggtccctc
      961 accccccagc cccctctccc aatgctggga gagggggagc acgaaggaac gcttgtaggg
     1021 gcgggtttca aacccgccct cgttcgagag gggcaatcgt gccggtcgaa cgggtgaact
     1081 gggccgacgt gccggtggag acggtctacc ccggcatcac gcgccaggtg gtgcagggcg
     1141 cgcggcagac gctggtgcgt tatgtctatc agcccggcgc cgtcttcccc cggcaccagc
     1201 atccccagga gcagatcacg gcggtgctca gcggcgcgat cgagttcgag gtcgccggcg
     1261 agcgccaccg gctgggcgcg ggcgaggtgg ccgtcatccc cggcgacacg ccgcacggcg
     1321 cccgtgtgct cggtgacgac gtcgtcgaga cgctcaacgc cctcagcccg cgccgcgacc
     1381 aggcgccggg gccggggtga tacggtccct cacgcttagc caggcggcca cacggcagcc
     1441 tacggcggga tctgcggcag taggtaaggc aattctactg aggagtgccc gatgatcgca
     1501 ccgacgcgtg atgacctgca ccacttcatc gaccaactca gcgacgagga tctccgcctt
     1561 gccgccggcg tgctcctcac gctgctggcc gagcgcgacc cgcttctcgc cgcgctgctg
     1621 gtggcgcccg aggacgacga gccgctcacc cccgacgagc tggcagccat cgaacggtcg
     1681 cgagccgagc tggaccgcgg cgcgtccgtg ccgtgggagc aggtccgccg cgagctgctc
     1741 gaggaatagc gcggtgtcgt ggcaactggt actccccgag tcgatccgga cccagttgcg
     1801 cgaactgccg cgggaggatc gccaggccat cggccgcgcc atcgaccaac tggtgcgcga
     1861 ccccggccag gccgacatcc gcaaacttcg cggccggccg gactggcgcc tgcgggttgg
     1921 ccgctggcgg gtgatcttcg agatgaacac ccgcgccagc gagatccacg tgctcgcggt
     1981 cgacgatcgc aaggatgtct accggtgagc ccagcggagc ctggtcccta cgctcaacac
     2041 cctcagcccg cgccgcgacc aggcgccggg gccggggtga tggactctcg caccgagatg
     2101 ccgaccgaca acgatgggtg gcccatgagg acggatccct ccactccggt cgggatgaca
     2161 ggggaggggc gcagcctacc ggacgagac
//
LOCUS       DASUBI010000521         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1012681, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000521 DASUBI010000000
VERSION     DASUBI010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012681"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFU72_10055"
     CDS             complement(<1..456)
                     /locus_tag="VFU72_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5423871.1"
                     /translation="MDGRRDDTAYWLGFHLVPGIGATRLARLIDSFGSLEAAWYASQP
                     ALRASGLNERAAATLVKTRSELSLDAELARVERAGVTILTMADDAYPRLLREIPSPPP
                     VLYVRGELTPADDGGVGIVGTRRASAYGREMALRLADGLARAGVTVVSGL"
     gene            578..754
                     /locus_tag="VFU72_10060"
     CDS             578..754
                     /locus_tag="VFU72_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423872.1"
                     /translation="MLLIWLVGFWLVTPTDPRHDGVYDYTELLKNGGFHTRAWLDDRV
                     EPPVDDKGQQDRER"
     gene            970..1731
                     /locus_tag="VFU72_10065"
     CDS             970..1731
                     /locus_tag="VFU72_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU5423873.1"
                     /translation="MRPNELKRKLRAGQTVVGCFVGYPSAETVEICGLAGYDFVMIDA
                     EHGPITPESAYHMVLAAEASGTTPLVRVWQNLQPVILRYMDTGAAGAMVPQVNSPEEA
                     EAVVRAVKYHPHGRRGMAGVRAAGFGLPQPLRSYIEEANRETMVIVQVETLQTVEALP
                     RILEVPGIDVLYVGPNDLSQSMGYPGQTDHPEVQAAIDRTIASARGHDVTLAMFVGSA
                     EAARREIARGFQMIGLSAATILGSASRQLLDDITR"
     gene            1764..2693
                     /locus_tag="VFU72_10070"
     CDS             1764..2693
                     /locus_tag="VFU72_10070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5423874.1"
                     /translation="MDSPLFPYAYFEGQVVPSDQAKVSIATHALQYGTGAFGGIRGYL
                     DKDGKTINIFRLPDHTARLLQSGLIIRAYVDYTPEKLAGVITELVCRNKPTRNVYIRP
                     FIYKASLELTPKLGGLKDELAIYMIALDDYLDLSKPLRLGVSSWRRIEDESIPSRAKI
                     TGAYINSSLAKDEASEAGYDDAIMLNHAGKVAEVSGANLFMVRHGMLITPPLTAGILE
                     GITRRSLLEFARDAGIPTEVRTIDRSELYVAEEVFLCGTGLQIGAVGQIDGRTIADGQ
                     RGPVTAKMQQTFFDLVRGEESPYRRYLTRIPIE"
     gene            3015..>3920
                     /locus_tag="VFU72_10075"
     CDS             3015..>3920
                     /locus_tag="VFU72_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423875.1"
                     /translation="MPSSTKIVEGETAEPRTLNPVLAADPLSDELSHLVFDGLVLVDP
                     QTGKPVGDLAETWDISDDRKSYTFHLRPGVRWSDGTAFSAADVVFSYNLMLNEDARSP
                     RYSRLKQQVAQVSARDATTVLITLQQPDAAFLTTLATLGIVPEHVLGSVLPEQIVSDP
                     FGIALTVGTGPFTLQSWAPGQELTFTRNASSFRPAQVAEYVYRVVTPDELGAGLADGS
                     IDWGPIDPALLDKAKGDRKIKVVSLPGFELEAVALQLDPAKSQLFLDPRVRQALLLAL
                     DRDALVAQVWHGQARVAGGTIPPASW"
BASE COUNT          627 a         1414 c         1290 g          589 t
ORIGIN      
        1 cagcccactt accacggtga ccccggcccg cgccagcccg tcggccagcc gcagcgccat
       61 ctcgcgtccg taggcgctgg cgcggcgcgt cccgacgatc cccaccccgc cgtcgtcggc
      121 cggcgtcaac tcgccgcgca cgtagagcac cgggggcggg ctggggatct cgcgcaagag
      181 ccgcgggtag gcatcgtcgg ccatcgtcag gatcgtcacc ccggcgcgct cgacccgcgc
      241 cagctcggca tcgagcgaga gctcggagcg ggtcttgacc agcgtggcgg cggcccgctc
      301 gttgagcccg ctggcgcgca gggccggctg gctggcgtac caggcggcct ccaggctgcc
      361 gaaggagtcg atcaagcgcg ccaggcgggt ggctccgata cccggcacca ggtgaaagcc
      421 cagccagtag gcggtgtcgt cgcgtcgccc atccataggc gcaaggttat acgggagcca
      481 tgcaacccgc gtcaacgcgc ggtggcggtc gagtggcgat ttgggacgga ggggctagac
      541 cggcgcggtg tggacgcgtc tacacctgct tcgaacggtg ttgctgatat ggctggtcgg
      601 cttctggctg gtcacgccga ccgatccccg gcacgacggg gtctacgact acactgagct
      661 cttgaagaat ggcggcttcc acacccgcgc ctggctcgac gacagggtgg agccgccggt
      721 ggacgacaag gggcaacaag atcgggagcg ttgagagcgt tggcgcgtgt aatcggcggg
      781 aagattgctg ttctccgtgg ttgttcgtgc cgcccggcgc ccgggaattc cattcccggg
      841 tgtcaagcgc cgccggaccc tcacctcaac ggccaaccgc ccccgatcgc ccgtgggtcc
      901 tcgcacccca ctctcgcccc gacacgcgcg cgacccatgc tagaatgccg gcgtgagaag
      961 ggagggtcgg tgcggcccaa tgagctgaag cgcaagctgc gcgcgggcca gacggtggtc
     1021 ggctgcttcg tcggctaccc ttccgccgag acggtcgaga tctgcggcct ggccggctac
     1081 gacttcgtca tgatcgacgc cgagcacggc ccgatcacgc ccgagtccgc ctaccacatg
     1141 gtgctggccg ccgaggccag cggtacgacg ccgctcgtgc gcgtctggca aaaccttcag
     1201 ccggtcatcc tgcgctacat ggataccggc gcggccgggg cgatggtgcc gcaggtcaac
     1261 agccccgagg aagccgaggc ggtggtgcgc gcggtcaagt accacccgca cgggcgacgg
     1321 ggcatggcgg gggtgcgcgc ggccggattt gggctgccgc agccgctgcg ctcgtacatc
     1381 gaagaggcca accgcgagac gatggtcatc gtgcaggtcg aaaccctcca gacggtcgag
     1441 gcgttgccgc gcatcctgga ggtgccgggc atcgacgtcc tctacgtcgg cccgaacgac
     1501 ctcagccagt cgatgggcta tcccggccaa accgatcatc ccgaggtgca ggcagccatc
     1561 gaccggacca tcgcgagcgc gcgaggccac gatgtgacgc tggcgatgtt cgtcggttcg
     1621 gccgaggcgg ccaggcgcga gatcgcccgc ggcttccaga tgatcggtct gagcgcggcg
     1681 acgatcctcg gcagcgccag ccgccagttg ctggacgaca tcacgcgctg agcgcgtcgt
     1741 tttcggaaag gacttgaacg agtatggatt ctccgctctt cccctacgcg tacttcgagg
     1801 gccaggtcgt cccgtccgat caggccaagg tcagcatcgc cacccacgcg ctgcagtacg
     1861 gcaccggcgc cttcggcggc atccgcggct acctcgacaa agacggcaag acgatcaaca
     1921 tcttccgcct gcctgatcac accgcgcgcc tgctccagtc cggcctgatc attcgcgctt
     1981 atgtcgacta cacgccggag aagctggccg gcgtcatcac cgagctagtc tgccgcaaca
     2041 agccgacgcg caatgtctac atccgtccct tcatttataa ggccagcctg gagctgacgc
     2101 cgaagctcgg ggggctcaag gacgagctgg cgatctacat gatcgcgctc gacgactacc
     2161 tcgacctgag caagccgctg cggctgggcg tctcgtcctg gcgccgtatc gaggacgagt
     2221 cgatccccag ccgggccaag atcaccggcg cctacatcaa ctccagtctg gccaaggatg
     2281 aggcgtcgga ggcgggctac gacgacgcca tcatgctcaa ccacgccggc aaggtggccg
     2341 aggtcagcgg cgccaacctc ttcatggtgc gtcacggcat gctgatcacc ccgccgctga
     2401 ccgccggcat cctggagggg atcacccgcc gctcgctgct cgagttcgcg cgcgacgccg
     2461 gcatcccgac cgaggtgcgc accatcgatc gcagcgagct gtatgtggct gaggaggtct
     2521 tcctctgcgg caccggcctg cagatcggcg ccgtcggcca gatcgacggc cgcaccatcg
     2581 ccgacggcca gcgcggcccg gtcaccgcca agatgcagca gaccttcttc gacctggtgc
     2641 gcggcgagga gtcgccctac cgccgctacc tgacgcgcat cccgatcgag tagcgacgta
     2701 atccacggca tctcgcgccc cgcctggaat cgaccggcgg ggcgcacgta tactcagcaa
     2761 ctgtgtcgag tcgtccgcac gcgaaccagc ctcaacggtc gcccacgcgc cggccagcgc
     2821 gccgccgcgc cctggcgcag agcctaccgc tgatcctggc attggcgctc tcggcctgtt
     2881 cgctgcatcg cccgggtggc gcacctcggc cgaccacggg ctcaccgtcg ccgctcggcg
     2941 ccgccgctac cccgctcgca ctcgcgaccg ccctggcagc ctcgccggct gccgccgcct
     3001 cgcccgtgcc gaacgtgccg tcgagcacga agatcgtcga gggcgagacc gccgagccgc
     3061 gcacgctcaa ccctgtgctg gcggccgacc cgctctcgga cgaactcagc cacctcgtct
     3121 tcgatgggct ggtactggtc gatccccaga caggcaagcc ggtgggcgat ctggccgaaa
     3181 cctgggacat cagcgacgac cggaagagct acaccttcca tctgcgaccg ggcgtgcgct
     3241 ggagcgacgg gaccgccttc tccgccgccg atgtcgtctt cagctacaac ctgatgctca
     3301 acgaggatgc ccgctcgccg cgctactccc ggctgaagca gcaggtggcc caggtcagcg
     3361 cgcgcgacgc taccactgtg ctgatcacgc tacagcagcc ggacgccgct ttcctgacca
     3421 ccctggccac gctgggcatc gtgccggagc atgtgctggg cagcgtgctg cccgagcaga
     3481 tcgtcagcga ccccttcggc atcgccctga ccgtcggcac tggccccttc acgttgcaga
     3541 gctgggcgcc cggccaggaa ctgaccttca cccgcaatgc cagctccttc cgccccgccc
     3601 aggtcgccga atacgtctat cgcgtggtca cgccggacga actgggagcc gggctggccg
     3661 acggctcgat cgactggggc ccgatcgacc ctgcgctgct cgacaaggcg aagggcgacc
     3721 gcaagatcaa ggtcgtcagc ctgcccggat tcgagctgga ggcggtggca ctgcagctcg
     3781 atccggccaa gagccagctc ttcctcgatc ctcgcgtgcg ccaggcgctg ctgctggcgc
     3841 tcgaccgcga cgcgctggtg gcgcaggtct ggcacgggca ggcgcgcgtc gccggcggca
     3901 ccatcccgcc cgcttcctgg
//
LOCUS       DASUBI010000522         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_923936, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000522 DASUBI010000000
VERSION     DASUBI010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923936"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..601)
                     /locus_tag="VFU72_10080"
     CDS             complement(293..601)
                     /locus_tag="VFU72_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423876.1"
                     /translation="MSTIEQRICPDCGSRRTQVVLRGLTGPTDESDQYFRCEDCGRVT
                     YEIVARTARELRIARISPGRQFRASGRLYEVRRVLKVGLDEYLVYLRPARDETATEVR
                     "
     gene            775..2331
                     /locus_tag="VFU72_10085"
     CDS             775..2331
                     /locus_tag="VFU72_10085"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5423877.1"
                     /translation="MRDQRTGAGDVGGLHQLPPEAQQLLAQRTLIIASNRGPIEYTLE
                     ADGSLTARRGAGGVVTAMSAAARYSNPIWVASAMTEGDRLRAELAAAGSEPFVVPPDS
                     DFRLRFVVSDPEAFLEYYNQISNPLLWFLQHYLWDTPRSPDVDAATWHAWNHGYVEVN
                     HQFASEIVAAAAASEQPPIVMLQDYHLYLTPGFLRELRPDLTIQHFIHIPWPDPDYWS
                     LLPRPIRQAICHSMLQNDIVGFQTRHFARSFMYTCEANLDGADIDYAGRAVRYQGRTT
                     WVRAYPISVDTDEVRGVARSEDAERHTQHLRNFHNEFTVVRVDRAEPSKNVVRGFLAF
                     DRFLELHPEFVGRVNFLAFLVPSRLEVLEYIDYLDDINTIVGRVNTKYANAAEYNGVP
                     WQPITLFIGDDYPRALAGLSHYDALLVNAIFDGMNLVAKEGVLLNQRPGVLILSEGAG
                     AYMQLGPYSLTISPTDIEGAAEAIYQALTMPEEQRAAWQREMRVIVERDDILSWIYSQ
                     LRDLAQRVEG"
     gene            complement(2350..3246)
                     /locus_tag="VFU72_10090"
     CDS             complement(2350..3246)
                     /locus_tag="VFU72_10090"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEU5423878.1"
                     /translation="MWIDESDDAKRCVIHIVSDGSGATAEAVVDAATVQYPGVEFEIV
                     RHPGVRTREQVLTVVSQAIRGHHIIVHTIVITSIRRLLLRECRQRVIPQVDLTGPLLG
                     QISQHVGMRPLLRPGVSHGLSDEYFRRVDAIQYSVRHDDGQGLEGLGDADIVLVGVSR
                     SSKTPLSIYLSLRGWKVANIPVVLGVEPPRQLDDIDQTRVVGLMINKDELVRIRQHRV
                     KSLGGLAHGEYADPEKVAEELAYCRRLIRRGYPWPIVDVTGKSVEESAKEIIALIESQ
                     RVRSSFSELEFPEEDLFPFLKE"
     gene            complement(3392..>5331)
                     /locus_tag="VFU72_10095"
     CDS             complement(3392..>5331)
                     /locus_tag="VFU72_10095"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5423879.1"
                     /translation="RRSVRRRRRMLCLKGECGLGEAPKTGSNRERAPRVLVPRWLSVL
                     ALCVVLGATCALSASPIGARASQAAPASRADLLGVSGNAFPWDGSFGKLRPLLDQSHA
                     GWARVELRWEQIEPSRGDWTWSGSDALVQGYHDLGMQELGLLVYSVGWASGVGGTQPV
                     FGPPSDLAAWQEYVRQTVTRYKGTIHAWEIWNEPDTSWAWDGQDGGDPATYLQLLVRA
                     DEAIKAVDPSATVVTGGVTGTARGAAFINTLLDLGGGQYFDMVGIHGYIDPNAEDVYY
                     TIIWPLLRQAAQRAGKPIWITEFGWSSNSSGPAAVGSEAAQANWIARELPMLFSLGNV
                     AHISLFQFRDPSDQPNTFGLVRPDGSKKPAYTVYQTYAAQLAGLRVQALVSPGDGTIR
                     DSRFSGNGRTLDVLWDEAGGRVATLSAGASAQVIHLDGSQQTLDATDGSVSIPVDNDP
                     VLVVRTGVASLESTGSGQCQAFAVTHQTLCGLFLDFWQRYGGLEIFGYPLSGEIQENG
                     RTVQYLERAKLEYHPESTDPDWRVVGELVGRSVTAGRERETAFQPLSSMASDAHCTAY
                     SETGHTLCNGFRAYWQQHGGLWLFGYPISQEFQEQNPDTGQVYTVQYFERARFEYHPQ
                     NPEPYTVQLGRLGAQLYDQRY"
BASE COUNT          889 a         1778 c         1755 g          909 t
ORIGIN      
        1 ctcggacaat gagcaggacg gatccctcga ctgcgctcgg gaccacaggg aaagtgatgt
       61 ggtctaccgg acgaggcatt tcgtctcctc gtcagcccca aatctcaccc tgtcatcctg
      121 agcgcagtcg aaggatccgt cttgcggtgc ctcgatgctg ctccgtcagc caccaaaacc
      181 cggttgacgt cgggcgttcg cgggtgtgcc gttttgcggt ctggagtcgg gtcgtttagg
      241 atggaagcgt gtggggcgca cggctccgcg cgggagggca tccgaactcc gcttaccgga
      301 cctcggtcgc ggtctcgtcc ctggctggac gcagatagac gagatactca tccagtccga
      361 ccttgagtac ccggcggacc tcgtagagcc ggccactggc gcggaattgc cggccgggag
      421 agattcgcgc gatgcgcagt tcacgcgccg tacgcgcgac gatctcgtag gtcacgcggc
      481 cgcaatcctc acagcggaaa tactggtcgg actcatccgt cggcccggtc aggccgcgca
      541 gcaccacctg agttcgccgg ctgccacagt cgggacagat gcgttgctca atggtactca
      601 cgatcgccgg tcccctcctg ctgtcgcgca atcgagacgc ccggattgta cgtgatcatc
      661 cttgggatgg gtacctgaac gttgtcggcg tgagaatagc ggctgatgcg gttcacggcc
      721 gtcgatgcgc ggtcgtcgca tcgttcaggg ttgccgcccg gtaggagagc gctcgtgaga
      781 gatcaacgaa ccggcgcggg ggatgttggg gggctgcatc agttgccgcc ggaagcccag
      841 cagttgctcg cgcaacgtac cctcatcatc gcttcgaacc gcggcccgat cgaatacacg
      901 ctcgaggcgg acggctcgct caccgccagg cgtggcgccg gtggcgtcgt cacggccatg
      961 agcgcggccg cccgctacag taacccgatc tgggtggcga gcgccatgac cgagggagac
     1021 cggctgcgcg ccgagctggc cgccgcgggc agcgagccct tcgtggtgcc gccggactcc
     1081 gacttccggc tgcgcttcgt cgtctcagac cccgaagcct ttttggagta ctacaaccag
     1141 atttccaatc cacttctttg gtttctacag cactacctgt gggacacgcc gcgctccccc
     1201 gatgtcgacg ccgccacctg gcacgcctgg aaccacggct acgtcgaagt caatcaccag
     1261 ttcgccagcg agatcgtcgc ggcagcggcg gcaagcgaac agcccccgat cgtcatgctg
     1321 caggactatc acctctatct gactcccggc ttcctgcgcg agctgcggcc cgacctcacc
     1381 atccagcact tcatccatat cccctggccc gaccccgact actggagtct gctgccgcgg
     1441 ccgattcgtc aggcgatctg ccacagcatg ctgcagaacg acatcgtcgg cttccagaca
     1501 cggcacttcg cacgcagctt catgtacacc tgcgaagcca acctcgacgg cgccgatatc
     1561 gactatgccg ggcgcgcggt ccgttaccag ggccggacaa cctgggtgcg tgcctatccg
     1621 atctcggtcg acacggacga ggtgcgaggc gtcgcgcgga gcgaggatgc cgagcgacac
     1681 acgcagcacc tgcggaactt ccacaacgag ttcacggtgg tgcgcgtcga tcgggccgag
     1741 ccgagcaaga acgtggtgcg tggctttctg gccttcgacc gcttcctgga gctgcacccg
     1801 gagttcgtcg gacgggtcaa cttcctggcc ttcctggtgc cctcgcgcct ggaggtgctg
     1861 gagtacatcg attatctgga cgacatcaac accatcgtcg gtcgagtcaa caccaagtac
     1921 gccaacgccg ccgagtacaa cggcgtcccc tggcagccaa ttaccctctt tatcggggat
     1981 gactacccac gggcgctggc cgggctgagt cactacgacg cgctgctggt caacgcgatc
     2041 ttcgacggca tgaatctggt cgccaaagaa ggggtgctgc tcaaccaacg cccgggcgtg
     2101 ctgatcctct ccgagggcgc cggcgcctat atgcagctcg gcccctactc gctcaccatc
     2161 tcgccgaccg atatcgaggg agccgccgag gcgatctacc aggcgctgac catgccggag
     2221 gaacagcgcg ccgcgtggca gcgggagatg cgtgtgatcg tcgagcgcga cgacatcctg
     2281 agctggatct acagccagct tcgcgacctg gcccaacggg tcgaagggtg acgcgccgcg
     2341 ccgacgccgt tactccttga ggaacggaaa gaggtcctct tcggggaact ccagctcgga
     2401 aaacgatgag cggacgcgct ggctctcgat cagcgcgatg atctccttgg ccgattcctc
     2461 gaccgacttg ccggtcacat cgacgatcgg ccaggggtaa ccgcgccgga tcaggcggcg
     2521 gcagtaggca agctcctccg cgaccttctc ggggtcggcg tattcgccgt gcgccaggcc
     2581 gcccagcgat ttcacccggt gctgccggat acgcaccagc tcatccttgt tgatcatcag
     2641 cccgacgacg cgcgtctggt cgatgtcatc gagctggcgc ggcggctcga caccgagcac
     2701 gaccgggatg ttggccactt tccagccccg cagcgagagg tagatgctca ggggggtctt
     2761 cgacgagcgc gagacgccga ccagcacgat gtcggcgtcg cccagaccct ccagcccctg
     2821 gccgtcgtcg tgccgtaccg agtactggat cgcatccacg cggcggaagt actcgtcgct
     2881 gagaccgtgc gaaacgccgg gacgcagcag cggccgcata ccgacatgct gcgagatctg
     2941 gcccagcagg gggccggtca gatcgacctg ggggatgacc cgctggcgac actcgcgtaa
     3001 caacaggcga cggatgctcg tgataacgat ggtgtggacg atgatgtggt gcccacggat
     3061 cgcctggctg acgaccgtca gcacttgctc acgcgtgcgc acgccggggt ggcggacgat
     3121 ctcgaactcg acgccggggt actgcaccgt cgcggcgtcg acgacggctt ccgcggtcgc
     3181 gccgctgccg tcggacacga tgtgaatgac acagcgtttg gcatcatccg actcatcgat
     3241 ccacatggac acctcgaccc ttcccgagcg gtgactgcgt gtagagccta ccatctgagg
     3301 ggttcgggcg gcaggcgggc gcagcgagcg tcggggtcga ttccatcgac gcgtttggtg
     3361 ccgcgtgctg cttgggagcg acggtccggg ttcagtagcg ctggtcgtag agctgcgcgc
     3421 cgaggcggcc gagttgcacc gtgtagggtt ccgggttctg ggggtggtac tcgaagcggg
     3481 cgcgctcgaa gtactgcacg gtgtagacct gaccggtgtc ggggttctgc tcctggaatt
     3541 cttgactgat cgggtagccg aataaccaga gcccaccgtg ctgctgccag taggcccgga
     3601 agccgttgca caacgtgtgg ccagtctccg aataggcggt gcagtgggcg tcactggcca
     3661 tgctgctcag gggctggaag gccgtctccc gctctcggcc agcggtcaca ctgcgcccga
     3721 ccagctcgcc caccacccgc cagtcgggat cggtcgactc cgggtgatat tcgagcttgg
     3781 ctcgctccag gtactggacc gtgcggccgt tctcctggat ctcgcccgac agcgggtagc
     3841 cgaagatctc caggccgccg tagcgctgcc agaaatcgag gaagagcccg cacagtgtct
     3901 ggtgtgtgac cgcgaacgcc tggcactggc cgctgccggt actctccagc gacgctacgc
     3961 cggtgcgtac aaccagcacc gggtcgttgt cgaccgggat gctgacgctc ccgtccgtgg
     4021 cgtcgagcgt ctgctggctg ccgtcgagat gaatcacctg tgccgaggcc ccagcgctca
     4081 gcgtggcgac acgcccgcct gcctcatccc agagcacgtc gagcgtgcgg ccgttgccgc
     4141 tgaagcggct gtcgcgaatg gtgccgtcgc cgggggagac caacgcctgg acgcgcaggc
     4201 cggccagctg ggcggcgtag gtctggtaga cggtataggc cggtttcttg ctgccgtcgg
     4261 ggcgcaccag accgaaggtg ttgggctggt cgctgggatc ccgaaactgg aagagcgaga
     4321 tgtgcgccac gttgcccagg gaaaacagca tcggcagctc gcgggcgatc cagttggcct
     4381 gggccgcctc cgagccgacg gccgccggcc cgctgctgtt gctcgaccag ccgaactcgg
     4441 tgatccaaat cggcttgccc gcgcgctggg ccgcctgacg cagcagcggc cagatgatgg
     4501 tgtagtagac atcttccgcg ttggggtcga tgtagccgtg gataccgacc atgtcgaagt
     4561 actggccgcc acccaaatcg aggagcgtat tgatgaaggc ggcaccgcgg gccgtgccgg
     4621 tgacgccgcc ggtgacgaca gtggccgacg gatcgaccgc cttgatcgct tcatcggcgc
     4681 gcaccaagag ctggaggtag gtggccggat cgccgccgtc ttgcccatcc caagcccagg
     4741 aggtgtccgg ctcgttccag atctcccagg cgtggatggt gcccttgtag cgagtcaccg
     4801 tctggcgcac gtactcttgc caggcggcga gatcgctggg cgggccgaag accggctggg
     4861 tgccacccac gccgctggcc cagccgaccg agtagaccag cagccccagc tcctgcatgc
     4921 cgagatcgtg gtagccctgc accagcgcgt cgctgccgct ccaggtccaa tcgccccggc
     4981 tgggctcgat ctgttcccag cgcagctcga cgcgcgccca gcccgcgtgg ctctgatcga
     5041 ggagtggtcg gagtttgccg aagctcccgt cccaggggaa cgcgttcccg gagactccga
     5101 gcaggtcggc gcggctcgcg ggcgccgcct gcgacgcgcg cgctccgatt ggactcgcgg
     5161 agagggcaca tgtggcgccc agcacgacgc agagcgcgag cacggacagc cagcgcggta
     5221 ccagcacgcg cggggcgcgc tctcgattac tcccggtttt gggtgcctcg cccaatccac
     5281 attccccctt caggcacagc atacgccggc ggcgccgcac gctccgccgc c
//
LOCUS       DASUBI010000523         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_320267, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000523 DASUBI010000000
VERSION     DASUBI010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320267"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..2080
                     /locus_tag="VFU72_10100"
     CDS             155..2080
                     /locus_tag="VFU72_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5423880.1"
                     /translation="MRAEERIRLLALVETGFRPAHATSTGPAYAVATGDSQWFQDNIP
                     CQAACPAGTDVPRYIALLADGRAAESDALNREHNILSGCLGRVCARPCEAACRRGVVD
                     SPVAICSLKRAAYDLAPPRTPERPRRANGRSVGIVGAGPAGLAAARELARAGFRVVIY
                     ERHAVPGGMLWAGIPAWRLPREIIQDEVQAIVALGVEIRYGVQVGRQITLDELLAGHD
                     AVVLATGCQDSVQLGIPGEELAGVTGGLAFLEGVNLDGAGADYRGARVVTIGGGYASL
                     DCARTAQRLGAAQSVLTYRRDETAMPVAADELREAAREGVDFAFLATPKRILGEGGRV
                     TGVELERNRLVTRAAGRAELEPIPGSGFVLPCERVIVAIGQRQRDDLGLAGAITRLRW
                     PGVPAVDQELRTDHPRVWAAGDFAARPRDVISAIGDGKRAAASIDSTLRGAVPRAPEA
                     ELTSLSTPEFPSLRRLLAEGVADWTLTTLARRLDQDDDYLELPRQEMSLLSLAEREIG
                     APAGLGREVEQGFAPDLAADEARRCLQCQLNIFIDGNACILCNGCVEVCPQQCIEMAT
                     VDRLAAIDGDTKLAGAAGAAIGDSGAAMLIHEVDCVRCGRCVERCPTGCLTMEHFRVV
                     EPDAVLSVAGSRLAGAR"
     gene            complement(2063..>3167)
                     /locus_tag="VFU72_10105"
     CDS             complement(2063..>3167)
                     /locus_tag="VFU72_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5423881.1"
                     /translation="IEDALDSGDTVVFVVDPDGQVIDNPRGVKLEGVPIRAGIERALA
                     GTRDSRTVTINDVGAVRVLSLPIRDKDRVVGAAQALRSLEEHQRELALLQTLTLAGLG
                     LGVVIAVPAGLFLARRAMRPIRAAFERQRTFVADASHELRTPLTLIRANAELVLGDPQ
                     VPVAEVTPELTSILAEVDRTDRLVDDLLTLARADSGRLPLQREPHDLRALAATAAASM
                     SALASARGLSVDLAASPNCLVWVDGERLLQVIRILLDNAIKHTAPGGTIRLAVACSGK
                     EASLRVEDSGEGIPPGELERVFDRFYRIDKARSRAAGGTGLGLAIAKALVEAHGGRIT
                     LSSRLGVGTVVEVTLPLRAAPVPTAAPGSARSG"
BASE COUNT          420 a         1167 c         1165 g          415 t
ORIGIN      
        1 cgagccgccg gcgctccacg tgcgagagag cccgcgccgc ggtgagcgtt gcccgctgtg
       61 ccaccagctc aaatccaacc caaatacctc cggtccaggc tgggagcgac gtgggtggat
      121 cgcgcccgcg tgcctgagcg aatgaaggat gcgcatgaga gccgaagaac ggattcgcct
      181 gctggcgcta gtcgagactg gcttccgccc cgcccacgcc acctcgaccg gtccggccta
      241 cgccgtcgcc accggcgata gccagtggtt tcaggacaac atcccgtgcc aggctgcctg
      301 ccccgccggg accgacgtgc cgcgctacat cgcgctgctg gccgacgggc gcgccgccga
      361 gagcgacgcg ctcaaccgcg agcacaacat cctctccggc tgcctgggtc gggtctgcgc
      421 ccggccctgc gaggccgcct gccggcgcgg cgtggtcgac tccccggtcg cgatctgctc
      481 gctcaagcgg gcggcctacg acctggcgcc gccgcgcacg cccgagcggc cgcgccgcgc
      541 caacggacgc agcgtcggga tcgtcggcgc cggtcccgcc ggactggcgg cggcgcgtga
      601 gctggcgcgc gcgggcttcc gggtggtgat ctacgagcgg cacgccgtgc cgggcggcat
      661 gctctgggcc ggcatcccgg cctggcgcct gccgcgcgag atcatccagg acgaagtgca
      721 ggcgatcgtg gcgctcggcg tcgagattcg ctacggtgtc caggtcggcc ggcagatcac
      781 cctggacgag ttgctcgccg gacacgacgc ggtcgtgctg gccaccggtt gccaggactc
      841 ggtgcaactc ggcatccccg gcgaggagct ggcgggcgtg accggcgggc tggccttcct
      901 ggaaggcgtc aacctcgacg gcgcgggcgc cgactatcgg ggagcacggg tggtcaccat
      961 cggcggcggc tatgcctcgc tcgactgcgc ccgcaccgcc cagcggctgg gcgccgccca
     1021 atcggtgctg acctaccggc gcgacgagac ggccatgccg gtggcggcgg acgagctgcg
     1081 ggaggcggcg cgcgagggcg tcgacttcgc cttcctggcc acgccgaaac gcatcctggg
     1141 cgagggcggg cgtgtgaccg gcgtggagct ggagcgcaac cggctggtca cgcgcgccgc
     1201 cggccgggcc gagctggagc cgatccccgg ctccggcttc gtcctcccct gcgagcgcgt
     1261 gatcgtggcc atcggccagc gccagcgcga cgacctgggg ctggccggcg ccatcacgcg
     1321 cctgcgctgg cccggcgtcc cagcggtcga ccaggagctg cgcaccgatc acccgcgcgt
     1381 ctgggccgcc ggtgacttcg ccgcccgacc gcgcgacgtc atctcggcca tcggcgacgg
     1441 caaacgcgcg gcggcttcga tcgacagtac gctccggggc gccgtcccgc gcgcgccgga
     1501 ggccgagctg acctccctct cgacccccga attccccagc ctgcgccggc tgctggccga
     1561 gggcgtggcc gactggaccc tgaccacgct ggcgcgccgc ctcgaccagg acgacgacta
     1621 cctggagctg ccgcgccagg agatgtccct gctgtcgctg gccgagcgcg agatcggcgc
     1681 gccggccggg ctgggccgcg aggtcgagca gggcttcgcg ccggacctgg cggccgacga
     1741 ggcgcgccgt tgcctgcagt gccagctcaa catcttcatc gacgggaacg cctgcatcct
     1801 ctgcaacggc tgcgtcgagg tctgccccca gcagtgcatc gagatggcca cggtcgaccg
     1861 gctggccgcg atcgacggcg acacgaagct ggccggtgcg gccggcgcgg ccatcggcga
     1921 cagcggcgct gccatgctga tccacgaggt ggactgcgtg cgctgcggcc gctgcgtcga
     1981 gcgttgccca accgggtgcc tgacgatgga gcacttccgc gtcgtcgagc cggacgcggt
     2041 gctctccgtg gcagggagcc ggctagccgg agcgcgctga gcccggcgcg gcagtaggca
     2101 cgggggccgc gcgcagcggc agcgtgacct ccaccacggt gccgacgccg agccggctgc
     2161 tcagcgtgat gcggccgccg tgcgcctcga ccagcgcttt ggcgatcgcc agccccagcc
     2221 cggtgccgcc ggcggcgcgg ctgcgcgcct tatcgatgcg atagaagcgg tcgaagacac
     2281 gctccagctc gccgggcggg atgccctcgc cgctgtcctc gacgcgcaga ctggcctctt
     2341 tgccggagca ggcgacggcg agccgaatcg tgccgccggg ggcggtgtgc ttgatggcgt
     2401 tgtccagcag gatgcggatc acctgaagca gccgctcgcc gtcgacccag accaggcaat
     2461 tgggtgaggc tgcaagatcg acgctcaggc cgcgcgccga cgccagcgcg ctcatgctgg
     2521 ccgcggcggt ggcggccagc gcccgcagat cgtgcggctc ccgctgcagg ggcaagcggc
     2581 cgctgtcggc gcgcgccagg gtcaggaggt cgtcgactag ccggtcggtg cggtcgacct
     2641 cggccaggat gctggtcaac tcgggcgtaa cctcggccac cggtacctgc gggtcgccca
     2701 gcaccagctc ggcgttggcg cgaatcagcg tcagcggcgt gcgcagctcg tgcgaggcgt
     2761 cggcgacgaa ggtgcgctgg cgctcgaagg cggcgcggat cgggcgcatg gcgcgccgcg
     2821 ccagaaagag ccccgccggc accgcgatga ccacgccgag cccgagaccg gccagcgtca
     2881 gcgtctgcag caacgccagc tcgcgctggt gctcctccag gctgcggagc gcctgggccg
     2941 cgcccaccac ccgatccttg tcgcgtatcg gcagcgagag gacgcgcacc gcgccgacgt
     3001 cgttgatcgt caccgtgcgg ctgtcgcgcg tgcccgccaa cgcccgctcg atgccggccc
     3061 ggattggcac gccctcgagc ttcacgcccc gcgggttgtc gatgacctgg ccgtctggat
     3121 cgaccacgaa gacgacggtg tcgccggagt cgagggcgtc ctcgata
//
LOCUS       DASUBI010000524         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1274448, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000524 DASUBI010000000
VERSION     DASUBI010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274448"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFU72_10110"
     CDS             <1..720
                     /locus_tag="VFU72_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423882.1"
                     /translation="FALLWGGHVLGMLARPEARGGEEEALLDWAASRVAAERLIVLCR
                     DDDAALMARLQRRGFVVGQEELRLERDLASAIDEPRLPAGFRIRPLDVPGELAEWEDL
                     YRASIGPRERAIRKWRAYRSDPDYRPELDLVAVDGHGRIAAACTCTVASLELGRSSIK
                     EGRTEPIMVGADYRRLGLGRAIVLTGLLLLAARGLDVARLTMEPGNHPACRLYTSLGY
                     RPIYQALWYRQPRSVPPAQIR"
     gene            796..1773
                     /locus_tag="VFU72_10115"
     CDS             796..1773
                     /locus_tag="VFU72_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5423883.1"
                     /translation="MPSPRPTVRIGSNEPVDFHMHTRSSDGGWQTAQLIDYLADHGYR
                     VVAICDHDTMRSVPEAQARAAERGLVLIPGVEVTSWWDEKQWHLLVYGIEPQGESRFH
                     ALLAQQQANLRATGENSIRLFEANGKLLPQLDEVVAGRPLMPTYVLQAAIRAGHATNL
                     QTAHELLKSYGEQMKVDWPLAEVVETAHEAGGICVVGHPGRPDLGPVLDEGQLDRMLA
                     AIPIDGLETHYRSYSDADTARYRAMAETRGLLATSGSDSHAPGNPVYPRPHPARWVVP
                     FLARFGVEVEPFEGAPWQSGQLVVVNGISASHGTDAGRLERATVASSST"
BASE COUNT          335 a          783 c          811 g          339 t
ORIGIN      
        1 ttcgcgctgc tctggggcgg gcacgtgctg gggatgctgg cgcgcccgga ggcgcgtggc
       61 ggggaagagg aggcgctgct cgactgggct gcctcgcggg tggctgccga gcggctgatc
      121 gtcctctgcc gcgacgacga cgcggcgctg atggcgcggc tgcagcggcg cggcttcgtg
      181 gtcggccagg aggagctgcg cctggagcgc gacctggcgt ccgcgatcga cgagccgagg
      241 ctaccggccg gcttccgcat ccggccgctc gacgtaccgg gcgagctggc ggagtgggaa
      301 gacctctatc gtgcgtcgat cggaccacgc gagcgcgcga tccgcaagtg gcgcgcgtat
      361 cgctccgacc cggactaccg gcccgagctc gatctggtcg cggtcgacgg ccacggacgc
      421 atcgcggccg cctgcacctg caccgtcgcc tcgctggagc tgggccggtc atcgatcaaa
      481 gagggacgca ccgagccgat catggtcggc gccgattatc ggcgcctcgg gcttggacga
      541 gcgatcgtct tgaccggcct gcttctcctc gccgcgcgcg gcctggacgt cgcccggttg
      601 acgatggagc ccggcaatca ccccgcgtgc cggctctaca cctcgctcgg ctatcgcccg
      661 atctaccagg cgctctggta ccgccagccg cggtccgtgc cgcctgccca gatccgctga
      721 tcgtcggcgg cagatgccgc tataatcggg cgaggcgccc ggcgccagag ccagcctgag
      781 tgattgcgag gatcgatgcc cagcccccgg ccgacggtgc ggatcggctc caacgagccg
      841 gtcgacttcc acatgcacac ccgctccagc gatggcggct ggcaaaccgc ccaactgatc
      901 gactacctgg ccgaccacgg ctaccgggtc gtcgctatct gcgaccacga caccatgcgc
      961 tcggtgcccg aggcgcaggc gcgcgccgcg gagcgcgggc tggtgctgat tcccggtgtc
     1021 gaggtgacat cgtggtggga cgagaagcag tggcacctgc tggtctacgg catcgagccc
     1081 cagggcgaga gccgcttcca cgcgctcctg gcccagcagc aggccaacct gcgcgctacc
     1141 ggcgagaact cgatcaggct cttcgaggcc aacggcaagc tcttgccgca gctcgacgaa
     1201 gtcgtcgccg ggcgcccgct gatgccgacc tacgtgctgc aggcggccat ccgtgccggg
     1261 cacgccacca acctgcagac cgcgcacgag ctgctgaagt cgtacggcga gcagatgaaa
     1321 gtcgactggc cgctggcgga ggtggtcgag acggcgcacg aggcgggcgg catctgcgtc
     1381 gtcggccacc cgggccggcc ggacctgggt ccggtgctcg acgaggggca gctcgatcgt
     1441 atgctggccg caataccgat tgacgggctg gagacgcact accgcagcta cagcgatgcc
     1501 gacaccgcgc gctaccgcgc catggccgag acgcgcggcc tgctggcgac cagcggctcc
     1561 gactcgcacg cgcccggcaa ccccgtctat cctcggccgc acccggcgcg ctgggtggtg
     1621 cccttcctgg cgcgcttcgg cgtcgaggtc gagcccttcg agggcgcgcc ctggcagtcg
     1681 gggcagttgg tcgtggtcaa cggcatttcg gcttcgcacg gcacggatgc cgggcggctg
     1741 gagcgcgcga ccgtcgccag cagctcgacc tgaggggacc ttcagcgcga tcctaccatc
     1801 acgacgggcc agcgcggtct tgactgaggg tggtggccgg cggatcggac ggttcacctc
     1861 gtcggagaga gcacagtctg gttcaccgag aggccgcccg agacccgcgg cgccatgctc
     1921 ccaggtgcac gttgcccagc ggcgctcacg cgtgatgaac caaccctgcc tactgccagc
     1981 ggtataatgc gaccagcgat caaaccgatt tcgtcggggg ctcgtctcgt ggcttcacga
     2041 ggttgtgctg ggtgttcgat ggacttgacc gacacaccgg acgcgacgac gagcgggagc
     2101 gtgggccaac cgggccacga ggcgcgtcca agccggcgcc gatcgcgtct gacgtctcgc
     2161 tgggttctgc tggtcatcgc gctggttgtc gtcggactgg gcgtgatcct cgcgcggcac
     2221 gatctcgcaa gcggcggagg agatgagctc aaggcagagg ggacgctc
//
LOCUS       DASUBI010000525         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_504192, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000525 DASUBI010000000
VERSION     DASUBI010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504192"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFU72_10120"
     CDS             <1..524
                     /locus_tag="VFU72_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423884.1"
                     /translation="SFSVAAVTDLSVIDRRRVVSLCTSAFGRSFAGLFALVPPDSLHL
                     LARLDGRLVGHACWMTRWLQPEGSPPLRTAYVDAVAIAPDLQGHGLGSRLIARLNHEL
                     AGFEISGLSTSRVAFYRRLGWEPWRGPTAARTARGLEPSSDDTIMILRTPHTPPLDLD
                     GLLSIELRAGSVW"
     gene            complement(671..1144)
                     /locus_tag="VFU72_10125"
     CDS             complement(671..1144)
                     /locus_tag="VFU72_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5423885.1"
                     /translation="MLTRYPMVEFTQFFNDVNRLFNAAQSGGQLSAALQPLADLYETG
                     DELVLKALVPGITPENLNVTIEQNVLTITGRYPQFVSPEHARQVTWYRKEIPSGRFTE
                     QLRLPVPVDASQIQAAFNDGILTLTMPKAEHARARQIQIQSNTAQPALTQGSHSA"
     gene            complement(1430..1762)
                     /locus_tag="VFU72_10130"
     CDS             complement(1430..1762)
                     /locus_tag="VFU72_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423886.1"
                     /translation="MAEPILGRFDFDTEIERFAPGEGASGRRAETLIKTEGLRVVLVT
                     LRAGAALHEHTAPGPITIQALRGHLTVSLEGADPVPLRRDQFIAIASGVRHSVRAEDA
                     AAFLLTIG"
     gene            complement(1824..>2022)
                     /locus_tag="VFU72_10135"
     CDS             complement(1824..>2022)
                     /locus_tag="VFU72_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEU5423887.1"
                     /translation="LMVLLRAPHPPAGATTLIVSLGILTAPHQLAILMVAVVLLTLQA
                     FVINRLAGLPYPLWSTPRDGA"
BASE COUNT          300 a          684 c          695 g          343 t
ORIGIN      
        1 tctcgttctc ggtcgccgcg gtcacggacc tctcggtgat tgaccggcgc agggtcgtct
       61 cgctctgcac gagtgccttc ggtcgtagct tcgccgggct cttcgcgctg gtgccgcccg
      121 attccctgca cctgctggcc cggctcgacg ggcggctggt tggacacgcc tgctggatga
      181 cgcgctggct gcagccggag ggctcgccgc cactgcgcac ggcctacgtc gacgcggtcg
      241 cgatcgcgcc ggatctgcag ggccacggtc tggggagccg cctgatcgcg cggctcaatc
      301 acgagctagc cggcttcgag atcagcggcc tctcgaccag ccgggttgcc ttctaccggc
      361 gcctcggctg ggagccctgg cgcgggccga ccgcggcccg caccgctcgc ggcctggagc
      421 cctcgtccga cgacaccatc atgatcctgc gcaccccgca cacgccgccg ctcgacctcg
      481 acggactcct cagcatcgag ttgcgcgccg gcagtgtctg gtgagcgacg cccggccggg
      541 cggccgggca ccggatcgac accccgcatc atcatcccgc cgcgcagcgg cgggatgacc
      601 agcactcgtg ccatgcgaaa gggcccgccc cgagctcggg gcgggccggc tgtcggcgcg
      661 ctgccgcggt ctatgccgag tggctcccct gagtgagcgc cggctgcgcg gtgtttgact
      721 gaatctggat ctgccgcgcg cgggcgtgct cggccttcgg catcgtcagg gtcagaatgc
      781 catcattgaa ggctgcctgg atctgcgagg cgtcgaccgg gaccggaagt cggagctgct
      841 cggtgaaccg gccgctcggg atctccttgc gataccaagt gacctggcga gcgtgctccg
      901 gcgaaacgaa ctgcgggtag cggccggtga tcgtgagcac gttctgctcg atggtgacgt
      961 tgaggttttc gggggtgatg ccaggaacca acgccttcag caccagctcg tcgccggtct
     1021 cgtagaggtc ggccagcggc tgaagtgccg cgctcaactg cccgcccgac tgagcggcgt
     1081 tgaacaggcg attgacgtcg ttgaagaact gggtgaactc gaccatagga tagcgggtca
     1141 acatggcgac aacgcctcct tccaatcgtc ccgctccagt catccaaccg gcggtcttgc
     1201 atccgaccgc ctccgcccaa tttaataccc catgattcgg atcgtgtcaa ggggttggtg
     1261 tggtattgat gaggtaaggt atgctgtagc actccatgtt tctattgatt ggccatcagg
     1321 atttgatccg ccggcataca ggcgggcgag cgttcgagcc ggtccgttct tccggccccg
     1381 gcagcaagac agggcgagcg ctgccccgcg gcatggtcag gtgagggtgc tacccaatcg
     1441 tcagcaggaa ggcggcggcg tcctcggccc gcaccgagtg acgcacgccg ctcgcgatgg
     1501 cgatgaactg gtcgcgccgg agcggcaccg ggtccgcgcc ctccaggctc accgtcaggt
     1561 ggccgcgtag cgcctggatc gtgattggcc caggcgcggt gtgctcgtgc agcgcggcgc
     1621 cggcgcgcag cgtcaccagc accacgcgca gcccctcggt cttgatcagc gtctcggcgc
     1681 ggcgcccgct ggcgccctcg cccggcgcga agcgctcgat ctcggtgtcg aagtcgaagc
     1741 ggcccaggat cggttcagcc atcatcggtc ctttctgcat atcaatacat gctccggcct
     1801 gcgcgtcgat catcttcgcg ggctcaggcg ccgtcccgcg gcgtggacca gagcgggtag
     1861 gggagcccgg ccaggcggtt gatcacgaac gcctggagag tcagcagcac gaccgcgacc
     1921 atcaggatag cgagctggtg aggcgcggtc aggatgccga gcgagacgat cagggtggtc
     1981 gccccggcag gtggatgcgg cgcccgcagc agcaccatca gc
//
LOCUS       DASUBI010000526         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_612214, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000526 DASUBI010000000
VERSION     DASUBI010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612214"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2022
                     /locus_tag="VFU72_10140"
     CDS             <1..>2022
                     /locus_tag="VFU72_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5423888.1"
                     /translation="ARGREWVVLPESADDLLVVRPLGGTDDEVTGLYLPLEEVRPASF
                     DLPDAAQVGDYRSGRLLRDAVRLGFRSSAGPFRSFAQLAVEPRPYQLVPLLLALKLDP
                     VRLLVADDVGIGKTVEAGLIARELLDRGEIRRLAVLCPPHLAEQWQAELRAKFHLDAV
                     AVLASTATRLERECAATESLFAHYPFVVVSTDFIKADRRRAEFVNHCPEFVIVDEAHT
                     CAFGGAGRGGQHQRHDLVRRLAERPDRHLVLVTATPHSGNETAFRSLLGFLDPAFGNL
                     PDDLSGPAHGADRRRLAAHLVQRRRADIRHYLQADTPFPAREEREVTYRLSPAYRQLF
                     DRVLRYAREIVRDPAGGQQRQRVRWWSALALLRSLGSSPAAAAATLRSRASTADAATV
                     SEADELGRRTILDLEDGEAGEFPDAIPGSDSEEPGTPGRRRLLDLAREAEALAGENDQ
                     KLLGVVPELRGLLAAGYHPIVFCRFIPTAEYLAAELRARLPKGVAVAAVTGTLPPAER
                     ETRVAQLLDAPQRVLVCTDCLSEGINLQEGFDAVVHYDLSWNPTRHEQREGRVDRFGQ
                     SRPTVRTLTYYGLDNPIDGIVLDVLLRKHKTIRSSLGVSVPVPVDTNQVVEAIFEGLL
                     LREQAGAAAAQLLPGLEAYFAPQKDALFQAWDDVTARERRSRTVYAQETI"
BASE COUNT          270 a          761 c          708 g          283 t
ORIGIN      
        1 gcgcgcgggc gcgagtgggt ggtgctgccg gagtcggcgg acgacttgct ggtggtgcgg
       61 ccactcggcg gcacggacga cgaggtgacg gggctgtacc tgccgctgga ggaggtgcgg
      121 ccggcgtcct tcgatctgcc ggacgcggcg caggtgggcg attatcgttc cgggcggctg
      181 ctgcgcgacg cggtgcgcct cggctttcgg tccagcgcgg ggccgttccg ctccttcgcg
      241 caactggcgg tcgagccgcg cccctatcaa ctcgtgccgc tgctgctggc gctcaaactc
      301 gacccggtgc ggctgctcgt cgccgatgac gtcggcatcg gcaagacggt ggaggcgggc
      361 ctgatcgcgc gcgaactgct ggaccggggc gagattcgcc gtttggccgt cctctgcccg
      421 ccgcacctgg ctgagcagtg gcaggccgaa ctgcgcgcca agttccacct cgacgccgtc
      481 gcagtgctgg cgagcaccgc cacccggctg gagcgcgagt gcgccgcgac cgagtcgctc
      541 ttcgcccatt atcccttcgt cgtcgtctcg accgacttca tcaaggccga ccgccggcgc
      601 gccgagttcg tcaaccactg ccccgagttc gtgattgtgg atgaggccca cacctgcgct
      661 ttcggcggcg cggggcgcgg gggtcagcac cagcgccacg acctggtgcg gcggctggcc
      721 gagcggccgg accgccacct ggtgctggtg acggcgacgc cgcacagcgg caacgagacg
      781 gctttccgct cgctgctcgg cttcctcgac ccggccttcg gcaacctgcc cgacgacctc
      841 agcggcccgg cgcacggcgc cgaccgccgc cgcctggccg cgcacctggt gcagcgccgc
      901 cgcgccgaca tccgccacta cctgcaggcc gacacaccct tcccggcgcg cgaggagcgc
      961 gaggtgacct accgcctcag cccggcctac cgccagctct tcgaccgcgt gctgcgctac
     1021 gcgcgcgaaa tcgtccgcga ccccgccggg ggccagcagc gccagcgcgt gcgctggtgg
     1081 tcggcgctgg ccctcctgcg ctcgctcggc tccagcccgg ccgccgccgc cgctacgctg
     1141 cgcagccgcg ccagcaccgc cgacgccgcg acggtgagcg aagccgacga actgggccgc
     1201 cgcaccatcc tcgacctgga ggacggcgag gcgggcgagt tcccggacgc catccctggc
     1261 agcgacagcg aggaaccggg cacgccaggc cgccgccgcc tgctcgatct ggcgcgcgag
     1321 gccgaggcgc tggccggcga aaacgaccag aagttgctcg gcgtcgtgcc ggaactgcgc
     1381 gggctgctgg cggccggcta ccacccgatc gtcttctgcc gcttcatccc caccgccgag
     1441 tacctggccg ccgagttgcg cgcccgcctg ccgaaagggg tggcggtcgc cgccgtcacc
     1501 ggcacgctgc cgcccgccga gcgcgagacg cgcgtggcgc aactgcttga tgcgccgcag
     1561 cgcgtcctcg tctgcaccga ctgcctcagc gaaggcatca atttgcagga aggcttcgac
     1621 gcggtcgtcc attacgacct ctcctggaac ccgacccgtc acgagcagcg cgagggacgc
     1681 gtggaccgct tcggccagtc gcgtcccacc gtgcggacgc tgacctacta cggcctcgac
     1741 aacccgattg acggcatcgt gctcgacgtg ctgctccgca agcacaagac gatccgctcc
     1801 agcctcggcg tctcggtgcc ggtgccggtg gacaccaacc aggtggtcga ggcgatcttc
     1861 gaagggctgc tgctgcgcga gcaggccggc gcggcggcgg cgcaactgct gccgggcctg
     1921 gaagcctatt tcgcgccgca gaaagacgcg ctcttccagg cgtgggacga cgtgaccgcg
     1981 cgcgagcgcc ggtcgcgcac cgtctacgcg caggagacga tc
//
LOCUS       DASUBI010000527         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1304921, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000527 DASUBI010000000
VERSION     DASUBI010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304921"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="crcB"
                     /locus_tag="VFU72_10145"
     CDS             <1..436
                     /gene="crcB"
                     /locus_tag="VFU72_10145"
                     /inference="COORDINATES: protein motif:HMM:NF010824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5423889.1"
                     /translation="ERGARVPGGRGGDQPRPADAGDGMSLLVVAGVGALGGFGAIARF
                     LLDGSVSGRLGRGFPFGTLAVNVSGAFVLGALAGATLSDTAYRLAGLGLIGGFTTYST
                     WALESHRLAEDGALRHGLLNLAVSLALGIPALWAGRHLGAAL"
     gene            507..1349
                     /locus_tag="VFU72_10150"
     CDS             507..1349
                     /locus_tag="VFU72_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5423890.1"
                     /translation="MLTVGVATVGLAVATRLIRNLSLSSALLATCVGVVLGPRVAGVL
                     VPPAHGGRHEVLLEAAEIALATSLMALARRFTPQQLRAATPRAARLVGFGMPGMALLT
                     GAGAWLIFDVSFWPGMLIGASLAATDPVVAAALVSGRLAERNIPAWLRHVLELEAGSN
                     DCLAVVLVLLPVAVLTHSEPVGPWLGDAAQEVGLAIGAGIASGALAALALRALRRAPG
                     LDDDALRPVPLGLTIAVLALVDLLGGSGVLGVFAAGLTFSSLVTAEERHTTQQTQGPP
                     RSSR"
     gene            1361..1801
                     /locus_tag="VFU72_10155"
     CDS             1361..1801
                     /locus_tag="VFU72_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423891.1"
                     /translation="MLFGASLPWSGWAALGWPAVAFAVWVLAVRRPPVVQLVLAGSRT
                     PARAIGFLGWFGPLGVASIFYATHLEPYDLSDQPTIFAAITAAICGSILAHTLTSTAG
                     VQRYAGRPALETLRHPLQRPSDRSQGPPRRRTPSSGNAPGSSGG"
     gene            complement(2088..>2602)
                     /locus_tag="VFU72_10160"
     CDS             complement(2088..>2602)
                     /locus_tag="VFU72_10160"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEU5423892.1"
                     /translation="PNTKARAEFEQFQRDQGITEDALHRPASQVTVKWREEHALAPAV
                     VERDGPLVDLDDRVLLEQLADTHAALLHQYKMPQLDLTQITSKIRPVTQATSRDLYDR
                     GAAGLLFRSNHDALRCIVLFEGRARLIADGRPTPMTKNHSALVRVCGEYGLVLRSPSS
                     SRTRRRKWQP"
BASE COUNT          290 a          918 c          942 g          452 t
ORIGIN      
        1 tgagcgtggt gctcgggttc ctggtggtcg cggcggcgac caacctcgtc cggcggacgc
       61 gggtgacggg atgagcctgc tggtggtcgc cggagtcggc gcgctcggcg ggttcggggc
      121 gatcgcccgg ttcctgctgg acggcagcgt ctccggccgg ctcgggcgcg gcttcccctt
      181 cggcacgctg gccgtgaacg tctcgggtgc gttcgtgctg ggcgcgctgg ccggcgcgac
      241 gctgagcgac accgcgtatc ggctcgccgg tctcggcctg atcggcgggt tcaccaccta
      301 cagcacgtgg gcgctggaga gccaccgcct ggccgaggac ggcgcgctgc gccacggcct
      361 gctgaacctc gccgtcagcc tggcgctggg catccccgcc ctgtgggccg ggcggcacct
      421 gggggcggcg ctgtgagcga cgactgctcg aggatctggg tcggtggtcg agcggggagg
      481 gcttcggtgt gacgctcgag gcttggttgc tgacggtcgg cgtcgcgact gtcggcttgg
      541 ccgtcgcgac gcgtctgatc cggaatctgt cgttgtcgag cgcgctgctg gcgacctgcg
      601 tgggtgtggt gctcggcccg cgggtggccg gcgtgctcgt tccgccggct catggcggtc
      661 gccacgaggt gctgctcgag gcagcggaga tcgcgctcgc cacctccttg atggcgctgg
      721 cccggcggtt cacaccgcag cagttgcggg cggccacgcc ccgagccgcc cggctggtcg
      781 ggttcggcat gccggggatg gcgctcttga cgggagccgg cgcctggctg atcttcgacg
      841 tgtccttctg gccgggaatg ctcatcggcg cgtcgctggc cgccaccgac ccggtcgtcg
      901 ctgcggcact ggtctccggc cggctcgcgg agcgcaacat cccggcgtgg ctgcgccacg
      961 tgctcgagct ggaagccggc tccaacgact gcctggccgt ggtgctggtg ctgttgccgg
     1021 tcgcggtact cacccacagc gagcccgtcg gcccatggct gggcgacgca gcccaggaag
     1081 tcggcctggc gatcggtgcg ggcatcgcct ctggcgcgct ggcagccctc gcgctgcggg
     1141 cgctgcgccg cgcgcccggc ctcgacgacg atgccttgcg gccggtgccg ctcggactga
     1201 ccatcgccgt gctggccctg gtcgacctgc tgggcggcag cggcgtactc ggcgtgttcg
     1261 ccgccggcct gacgttctcg agcctggtca ccgccgagga gcgccacacg acgcagcaga
     1321 cccaggggcc gccgaggagc tcacgctgat cgcggtgttc gtgctcttcg gcgcgtcgct
     1381 cccctggtca ggctgggcag cgctcggctg gccggccgtc gcgttcgccg tctgggtcct
     1441 cgccgtgcgg cgcccgccgg tcgtccagct cgtgctcgcg ggctcccgca cccccgcgcg
     1501 agccatcggc ttcctgggct ggttcgggcc gctcggcgtc gcgtcgatct tctacgccac
     1561 ccacctcgag ccatacgacc tcagcgatca gcccacgatc ttcgcggcca tcacagcggc
     1621 gatctgcgga tcgatcctgg cgcacacgct gacgtcgacc gccggtgtcc agcgctacgc
     1681 cggccgaccg gccctggaga cgctgcggca tccgctgcaa cgcccaagcg accgctcgca
     1741 gggccctccg cgccggcgca cgccatcgag cggcaacgcg ccaggctcgt caggaggcta
     1801 aagatcgccg gcgctcgtgc cgataccact gacatcggcc gcctcaaatg gcggctcttt
     1861 tcgttccggc gcccaaacgc ccggcccgct cgagggctgc cttgaaccgg acccgcatct
     1921 tggtcgcgtc gtagggccgg ccggtctgcg ggtgcgcgaa caccaggtca tcgtcgtgcg
     1981 gctcaggtcc acgtggggcc gcggcctgtt cgtcgccttc tctgcgtggc tcgagttcgt
     2041 ggcgagagct ctggctgttt ggtgcctcgg ttggtgggag gcgccagtca gggctgccac
     2101 tttcgccgcc gagttcgact cgaactgggg ctgcgcagga ccagcccgta ctcgccacag
     2161 acgcgcacga gtgctgagtg attcttggtc atcggggtcg gccggccgtc cgcgatcaag
     2221 cgcgctcgcc cctcaaagag cacgatgcag cgcaacgcgt cgtgattcga gcggaacagc
     2281 agtcccgctg ccccgcggtc gtaaaggtcg cggctagtcg cttgggtgac cggccggatc
     2341 ttcgacgtga tctgggtgag atcgagctgc ggcatcttgt actgatgcag cagcgcggcg
     2401 tgggtatcgg cgagctgttc cagcagcacg cggtcgtcca agtcgaccag cggtccgtcg
     2461 cgctcgacga cagccggtgc cagcgcatgc tcctcgcgcc acttcaccgt cacctggctc
     2521 gccggccgat gtagcgcgtc ctcggtgatt ccctgatctc gctggaattg ctcgaactcg
     2581 gctcgcgcct tggtgttcgg cc
//
LOCUS       DASUBI010000528         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1296147, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000528 DASUBI010000000
VERSION     DASUBI010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296147"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..616
                     /locus_tag="VFU72_10165"
     CDS             137..616
                     /locus_tag="VFU72_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423893.1"
                     /translation="MNVIRFTVIDDTASSFVGPCHAIKMLVAACSRDARTLGELLAFA
                     RPYDAAFVTGVQSGLAVFDEHNTAENFAAFRQQAGRLNPAELPPFRVLDESTRALSLT
                     PAGTGLIMFNLPARRIIQVQNSYADVLRTDRGRVRENGRPTRFLYHYELPSDWRILP"
     gene            complement(722..1744)
                     /locus_tag="VFU72_10170"
     CDS             complement(722..1744)
                     /locus_tag="VFU72_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU5423894.1"
                     /translation="MQRVSIVGLGLIGSSIGLGLRRWSSKDGKRDAVLEISGFDTSLD
                     VQNYAKKLKAVDRTEWNLPSAIASADFLVIATPVMAVREVFEVIAKHGKEGLVVTDTA
                     STKAEVMRWASEILPAHVNFIGGHPMAGKSLSVEGADAELFKSATWCVAPSVTATEEA
                     VQTVLGMIAALEAEALFVDPHEHDGFVGGISHLPFLLSAALMRTVSKDAGWRDMRKLS
                     STGFRDASRLASGSPEMHRDIALTNKESLLRWTDAAIAELASLRELVASGDEAALEAA
                     FNQARDARAEWMVQERGAGELVQDVDDELPNFNLGEQMQQMLFGGLFRRKPRTGREER
                     AGSPRR"
     gene            complement(1812..2345)
                     /locus_tag="VFU72_10175"
     CDS             complement(1812..2345)
                     /locus_tag="VFU72_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5423895.1"
                     /translation="MAEPGLTARLRQHSRRSGMMVGFSMAIVIAIVIAGFIWTYLRVG
                     PFLSDFIPSNAPPTAVAQVFATPGSGVAPVPAASTPTAAASPAAQTNLIPTPTPTPVW
                     RATHEIDSDGVNIRLRGGPSTSSQIIDSLPPGTRLQFLGEQQPSGGETWMKFETEDGK
                     IGWVRRIDVAAISGANP"
     gene            complement(2530..3687)
                     /locus_tag="VFU72_10180"
     CDS             complement(2530..3687)
                     /locus_tag="VFU72_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5423896.1"
                     /translation="MLIVIVLLLGLLVDRFLVPSTYTVVAPFALALVLVGRRFDRRIT
                     VLATAVILALFAGIAANDGVPGLVGLTDLVALGLIGYLGYRDAQGRGTVSSDHLRLET
                     EHRNLLQFLAAVAHDMRQPLTSIQGYAHYLQRSGGRDLPEYRALESMADAAQRLERLT
                     ADLADASAVGSGHFALKTGPVDLVALLRGLADDYQATTARHALVVDGPERLIGIWDGD
                     RLRQVFGNLIGNAIKYAPEGGEIRIDLYHAEHEIAVTVSDQGLGIGAEQMRFLFQPFS
                     RLRQSGAGADGTGLGLYISQGIALAHGGFIAVESEPGAGSTFRVVLPLVSGPQRPRQP
                     RPHRARHPVVVRPPRVPLAPDVDQPLDPPATEAAGAEEERPHEAGLRLPAS"
     gene            complement(3876..>4831)
                     /locus_tag="VFU72_10185"
     CDS             complement(3876..>4831)
                     /locus_tag="VFU72_10185"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5423897.1"
                     /translation="NLYATTAGPQGAPSLMVSAHSDEIGAIVRSIEPSGLIRLAPVGG
                     FLDKLAAARHVRVRGRRGVIGVKSGHLQSPEERGRVTPMRDLYVDLGFSSADEVRALG
                     IRVGDQITYDEPMVELANPDRVAGKALDNRIGCAVLVTLARRLQGAALPCTLHLVVAV
                     QEEVGLRGARVAAHRLNPTAAIVVDTAPAGGTPDVDYFKDLNMLIGGGPVLTLMSRGG
                     SGGYIASPALRDFVRGVAERQGITLQEAIFLGGNSDAASVHLEQDGIPTVAVNLARRY
                     SHSPVEMLDINDACGAVALLEAAVRAFGPEVDLGFLKAEWG"
BASE COUNT          774 a         1673 c         1648 g          736 t
ORIGIN      
        1 atcatggcgt ggtccgctcc cactcttttg cgccattcct gtctgtcgtg tcggtcaacc
       61 atatgataga ctttagtatc acgcgtctcg tgccggcaac cggggcgcga gtcagcagtc
      121 cgtgaggggg tccagcgtga acgtcatccg cttcaccgtc atcgacgaca cagccagcag
      181 cttcgtcggg ccgtgccacg ccatcaagat gctggtggcg gcttgcagcc gcgatgcccg
      241 caccctgggg gaactgctgg cctttgcgcg tccctacgac gccgcgttcg tcaccggggt
      301 gcaaagcgga ctggcggtct tcgacgagca caacacggcc gagaacttcg ccgcctttcg
      361 ccagcaggcc gggcgcctga acccggcgga actgccgccc tttcgggtac tggacgagag
      421 cacgcgcgcc ctgagcctga cacccgccgg caccgggctc atcatgttca acctcccggc
      481 gcgccgcatt attcaggtgc aaaacagcta cgccgatgtg ctgcgcacgg atcgcggccg
      541 ggtgcgcgag aatggccgcc cgacccgctt tctctaccac tacgagctgc cgagcgactg
      601 gcgcatcctg ccctagccgg cgctcggcgc ctcgcccgtg ccgctcggcc gccacgtgtg
      661 cgctcgcagc cgtcatcgcg acagcctctc cggtggtgac gccggccccg gtttcggcgg
      721 cctagcgccg cggcgatcct gcccgctcct cgcggccggt gcgcggcttg cgacggaaga
      781 gcccgccgaa gagcatctgc tgcatctgct cgccgaggtt gaagttcggc agctcatcgt
      841 cgacatcttg taccagctcg cccgccccgc gctcctgtac catccattcg gcccgcgcgt
      901 cgcgcgcctg gttgaaggcg gcttctaacg cggcttcgtc gccggacgcg accagctcgc
      961 gcagcgaggc gagctcggcg atggccgcgt cggtccagcg cagcagactc tccttgttgg
     1021 tcagcgcgat gtcacggtgc atctccgggc tgccggaggc cagccgtgac gcatcgcgaa
     1081 agccggtcga cgaaagcttg cgcatatcgc gccaaccggc atccttgctc accgtgcgca
     1141 tcagcgcggc cgagaggagg aagggaaggt ggctaatgcc gccgacgaag ccgtcgtgct
     1201 cgtgggggtc gacgaagagg gcttcggcct ccagcgcggc gatcatgccg agcaccgtct
     1261 gtaccgcttc ctcggtggcg gtgacgctcg gcgcgacaca ccaggtagcg ctcttgaaga
     1321 gctccgcgtc ggctccctcg acgctcagcg acttgcctgc catcggatgc ccgccgatga
     1381 aattgacgtg ggcagggaga atctcgctgg cccagcgcat gacctcggcc ttggtgctgg
     1441 ccgtatcggt gacgaccagt ccctctttac cgtgcttggc gatgacctcg aagacctcac
     1501 gcaccgccat caccggcgtg gcgatcacca ggaaatcggc gctggcgatg gcgctgggga
     1561 gattccactc cgtgcggtcg accgccttca gcttcttggc gtagttctgc acatcgagac
     1621 tggtgtcgaa gccggagatc tcgagtacgg cgtcacgctt gccatccttg ctcgaccacc
     1681 ggcggagacc gaggccgatc gacgaaccga tcaggccgag cccaacaatc gagactctct
     1741 gcatttgacg tccttcttcg tgacaaccgg aggttggagc gggtcagctt gactaaagta
     1801 tagcagaggg tctaggggtt ggcgccgctg atcgccgcca cgtcgatgcg gcgtacccag
     1861 ccgatcttgc cgtcttccgt ctcgaatttc atccaggtct caccgccgct gggctgctgc
     1921 tcccccagaa actggagccg cgtgccggga ggcagcgagt cgatgatctg cgagctggtg
     1981 gacgggccgc cgcgcagccg gatgttcacc ccgtcggagt cgatctcgtg ggtcgcgcgc
     2041 cagaccgggg tcggtgtcgg cgtcggaatc aggttcgtct gggctgcggg cgaggcagcg
     2101 gccgtcggcg tcgacgcggc aggaaccggc gcgacgccgc tgccgggcgt ggcgaagacc
     2161 tgggcgacgg ccgtgggagg cgcattcgag gggatgaagt cgctcagaaa cggaccgaca
     2221 cgcaggtagg tccagatgaa accggcgatc acgatggcga tcacgatcgc catgctgaag
     2281 ccgaccatca tgccggagcg gcgcgagtgc tgccgcagac gggcggtcaa gcctggttcg
     2341 gccattctat ccgagcaacc ttcctcatcc tgcccggctc gtcgcgcgat tatacactgg
     2401 ccgcgcggcg tgacttggcg cggagcctgg gcgcgcagcc ggctcctcgg ccgggttcga
     2461 ggtccgtgtc aagacctcca agagcaccca gccagcgcac gcgccatgcc gtgaccctgg
     2521 gaaggaacgc taggacgccg ggagtcgcaa gccggcctcg tggggccgct cctcttccgc
     2581 gccagccgcc tcggttgccg gcggatcgag tggttgatcg acgtccgggg cgagcggcac
     2641 gcgcggcggg cgcaccacga ccgggtgacg agcgcggtgt ggccgcggct gccgtgggcg
     2701 ctgcggcccc gagacgagcg gcaggaccac gcggaaggtg ctgccggcgc ccggctccga
     2761 ttcgacggcg atgaagccgc catgcgccag tgcgatgccc tgagaaatgt agaggccgag
     2821 gccggtgccg tcggcgcccg ccccgctctg gcgcaggcgc gagaagggct ggaagaggaa
     2881 gcgcatctgc tcggcgccga tgccgagccc ctggtccgag acggtcaccg cgatctcgtg
     2941 ttcggcgtgg tagaggtcga tccggatctc gccgccctcg ggcgcgtact tgatcgcgtt
     3001 gccgatcaga ttgccgaaga cctggcgcaa gcgatcacca tcccagatgc cgatcagccg
     3061 ctccggtcca tcgaccacca gcgcgtggcg cgcggtggtg gcctgataat catccgccag
     3121 cccccgcagc agggcgacca gatcgaccgg gccggtcttc aaggcgaagt ggcccgagcc
     3181 gaccgcgctg gcgtcggcca gatcggcggt cagccgctca aggcgctgcg cggcgtcggc
     3241 catcgactcc agcgcgcgat actcgggcag atcgcgcccg ccggagcgtt gcaggtagtg
     3301 cgcgtagccc tggatcgagg tcaacggctg gcgcatatcg tgggcgacgg cagccaggaa
     3361 ctgcagcagg ttccgatgct cggtttccaa gcgcaggtga tcgctcgaga ccgtgccgcg
     3421 cccctgggca tcgcgatagc cgagatagcc gatcaagccg agcgccacga gatcggtcag
     3481 gcccaccagg ccgggcacac cgtcgttggc cgcgatgccg gcgaagagcg cgaggatgac
     3541 ggcggtcgcc agcaccgtga tgcggcggtc aaaccggcgc ccgaccagca ccagcgccag
     3601 cgcgaagggc gcgacgacgg tgtaggtgga cggcaccaga aaccgatcga ccaataggcc
     3661 gagcagcagc acgatcacga tgagcatgac cgctccttga ggccaggtcc gcggccgtga
     3721 ccggcaacga gcgccggcgc cgcccacgaa ccagtacctt cgtactcaag tgtacgtacc
     3781 aattatgtcg ccgccggaag atggtgtcaa cacccggcgg aggcaaaact ggccccacgc
     3841 gcgcattctg tgaccatttg gcagcatccg agccgctagc cccactcggc cttcaagaag
     3901 ccgaggtcga cctccggccc gaacgcgcgc acggccgcct ccagcagcgc gaccgcgccg
     3961 caggcgtcgt tgatgtcgag catctcgacc ggcgaatgcg agtagcggcg ggccaggttg
     4021 acggccacgg tcgggatgcc gtcctgctcc aggtgcaccg aggcggcgtc cgaattgccg
     4081 ccgaggaaga tcgcctcctg cagcgtgatc ccctgccgct cggccactcc gcgcacgaag
     4141 tcgcgcaggg ccgggctggc gatgtagccg ccgctgccgc cgcggctcat cagggtcaga
     4201 accggcccac cgccgatcag catgttgaga tccttgaaat agtccacgtc gggcgtgccg
     4261 ccggccggcg cggtgtcgac cacgatggcg gcggtcgggt tgagccggtg cgccgccacc
     4321 cgggcgccgc gcagcccgac ctcctcctgc acggcgacca ccaggtggag cgtgcaggga
     4381 agcgccgcgc cctgcagccg gcgcgccagc gtcaccagca ccgcgcagcc gatgcggttg
     4441 tccagcgcct tgccggccac gcggtccggg ttggccagct cgaccatcgg ctcgtcatag
     4501 gtgatctggt cgccgacgcg gatgccgagc gcgcgcacct cgtccgccga gctgaagccg
     4561 agatcgacgt agaggtcgcg catcggggtc acgcgcccgc gctcctccgg cgactgcagg
     4621 tggcccgact tgaccccgat caccccgcgc cggccacgca cacggacgtg gcgcgcggcc
     4681 gccagcttgt cgagaaagcc gccgaccggg gcgagccgaa tcagaccgga cggctcgatg
     4741 ctgcgcacga tggcgccgat ctcgtcggag tgggccgaga ccatcaggct cggcgcgccc
     4801 tgcggtcccg ctgtagtggc gtagaggttg c
//
LOCUS       DASUBI010000529         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1344273, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000529 DASUBI010000000
VERSION     DASUBI010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344273"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..977
                     /locus_tag="VFU72_10190"
     CDS             45..977
                     /locus_tag="VFU72_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5423898.1"
                     /translation="MSVHPPSFGWIMQPAPFTVPEGSNPWDSDLARDLIRTNERFVEL
                     VQAAGFDTIWVEDHMGWGDKAHLECFTNMAWLAGRHPGLRYGTMVCGQAFRNPAYLAK
                     LATNMQLLTDGQFVLGLGAGNNGLEHQQYGFAFLPAGERLEQTEEAIQIMRALWDSCP
                     ASFTGRYYSIQDACSSPLPGERIPLMIGGGGEKKTLRLVARYADWWCCDVGPVALFER
                     KSRVLREHCAALGRDPAEIVQSQVVWVSVEDDPARLIRWDDLHIVAGTPDQVAAELRQ
                     FREAGVEHFQVRFMDYPGLAGARRFIERVIPCLT"
     gene            974..2101
                     /locus_tag="VFU72_10195"
     CDS             974..2101
                     /locus_tag="VFU72_10195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5423899.1"
                     /translation="MSPEKAPARPRRILVVFAWLMVGGEETEVRLLAKHLDPARYTID
                     VVACLRRPGMPEQTHRQLADLGVQVDRTPYALSFEDTVAYLAVKIARYDLVVACQGVP
                     DIHAALDLLPNPPPLIEHGGLVSEVTATPKRHTTRYVGVCASIRAAAAAAMPERPQHA
                     LEIPSMVDLAEFDPGARPAVRRAWGVDANMPVMGWIGRLDRKKRVEDFIRAAALVLVR
                     HPHARFVIVGGPDAYMPEYAGELRELAAHLELGAALSFLGDRPDVPRLLSGLDALVWL
                     SRGEGMPHVIAEAGAARLPVIATRDNGSEQQIIDGVTGLFVPHESPSAVAEGMDRLIA
                     GPALRARLGGNLRRKVEREYAADIVVPRWQALFDEVLAQRI"
     gene            complement(2170..3363)
                     /gene="glf"
                     /locus_tag="VFU72_10200"
     CDS             complement(2170..3363)
                     /gene="glf"
                     /locus_tag="VFU72_10200"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU5423900.1"
                     /translation="MKGFDYLVVGAGFAGSVLAERLASQLGKGVLVVDRRPHIGGNAY
                     DCYDDDGILIHRYGPHIFHTNSERIVAYLSQFTTWRPYEHRVLASVDGQLLPIPINLD
                     TVNRLYGTHFSSFDLEAFFKSVAEPREPIRSAEDVVISKVGRDLYEKFFRGYTRKQWG
                     VDPSELDATVTARVPTRTGRDDRYFADRYQIMPLHGFTRMFERMLAHPNIKILLNTDY
                     REVRDLIPHREVIYSGPIDEYFDYCYGRLPYRSLSFRFESHSSSRQQPVAVLNYPNDF
                     EYTRVTEFKHLTGQEHPRTTLVYEYPQANGDPYYPVPRPANRELYARYKALAESTPGV
                     HFVGRLATYQYYNMDQVVGQALALFNRLATPAVKASTETAGQGIVEQLIADLASEPAV
                     VPAEE"
     gene            complement(3360..4664)
                     /locus_tag="VFU72_10205"
     CDS             complement(3360..4664)
                     /locus_tag="VFU72_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5423901.1"
                     /translation="MAGEHAGARSSCRTRQTGQWSGVCVSRQQGGRMAPRYDVVCFSH
                     LRWDYVYQRPQHLLCRWARGHRVFYVEEPDYAGDEPRFVIREPAPGVHVVSSHLPESV
                     LEAGPAAWEALQRQLLDRLFAEQGIGSQVLWYYTPVALGYSDHLRPLATVYDCMDELA
                     AFRGAPARLARYERELFARADVVFTGGRSLYDAKRQQHPRVYCFPSSVDLEHFARARA
                     AMDEPADQRALVRPKLGFFGVIDERMDLELLDEVARRRPCWQLVLVGPVAKIDSASLP
                     RRDNLHYLGQKPYDTLPSYLAAWDVALMPFARNAATRFISPTKTLEYLAAGKAVVSTA
                     IPDVVQPYGALGLVRIADSVEAFVTAVEAALTEPAEVRLAAIDAMLAGTSWDRTWTGM
                     AELVNEIVVERAGRPSLLPLAGDGQRSGRENGPRGSSEARGR"
     gene            4835..>5838
                     /locus_tag="VFU72_10210"
     CDS             4835..>5838
                     /locus_tag="VFU72_10210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU5423902.1"
                     /translation="MPTFNQAAFIRRALDSLLAQVLEDWELVIVDDGSTDETGSVLAP
                     YREDRRVRYRRLERNRGLGAALNLGLSLARAPLVAYLPSDDVYYRDHLASLACTLDRH
                     PTAALAYAGVRHFYNRTVVGRIEGYPLQLVQVMHRRTEDRWLERDELVTDDLDRMYWS
                     KLRLRGGAQETGLVTCEWVDHPYQRHKVLREPVGGINPYRQRYGVSSPLRFHSTVGNR
                     IDEVEHFRRFRERPRTPPAPDGLKILLVGELAYNPERVLALEERGHTLYGLWMPDPYW
                     YNTVGPVPFGHVADLPRAGWQEAIRRVRPDVIYALLNWQAVPFAHHVLTENPGIPFVW
                     "
BASE COUNT          929 a         1957 c         1991 g          961 t
ORIGIN      
        1 tggttggcta agctgacgtg atcgggttga gaggagcatc gcgggtgagc gtccatcccc
       61 cgtcctttgg ctggatcatg cagccggctc cgtttaccgt gcccgaaggc agcaatccct
      121 gggacagcga cctggcgcgc gacttgatcc ggaccaatga gcgcttcgtg gagctcgtgc
      181 aggcggccgg cttcgacacc atctgggtcg aggaccacat gggctggggc gataaggctc
      241 acctggaatg cttcacgaac atggcgtggc tcgcggggcg gcacccgggg ctccgctatg
      301 gcacgatggt ctgcggccag gcgtttcgca atccggccta cctcgccaag ctcgccacta
      361 acatgcagct cctgacggat ggccagttcg tcctcggcct cggcgccggc aacaacgggc
      421 tggagcacca acaatacggc ttcgcgtttc tgccggcggg cgagcgactg gagcagacgg
      481 aggaggcgat ccagatcatg cgcgcgctct gggattcctg cccggccagc ttcacggggc
      541 gctactactc gatccaggac gcctgctcgt cgcccctgcc cggcgaacgc atccccctga
      601 tgatcggcgg tgggggcgag aagaagacgc tgcgcctagt cgcgcgctac gccgactggt
      661 ggtgctgcga cgtcgggccg gtggcgctgt tcgagcgcaa gtcgcgcgtc ctgcgcgagc
      721 actgcgcggc gctcggcagg gatccggccg agatcgtcca gtcgcaggtc gtctgggtaa
      781 gtgtggagga tgatccagcg cgcctgatcc gctgggacga cctacacatc gtcgccggca
      841 cgccggacca ggtggccgct gagctccgcc agttccggga ggccggggtg gagcactttc
      901 aggttcggtt catggattac cccggcctcg ctggcgcccg gcgcttcatc gagcgggtca
      961 tcccctgtct gacatgagcc cggagaaggc gccggcgcgg ccacggcgca tcctcgttgt
     1021 cttcgcctgg ctgatggtcg gcggcgaaga gaccgaagtg cgtctcctgg caaagcatct
     1081 cgatccggct cgctacacca tcgacgtcgt cgcctgcctg cgcaggccgg gcatgccgga
     1141 gcagacgcat cgccaactgg cggacctggg cgtgcaggtc gaccggacgc cctatgcgct
     1201 gtcgttcgaa gacaccgttg cctacctggc cgtcaagata gcacgctacg atctggtggt
     1261 tgcctgccag ggcgtgcccg acatccatgc ggcgctcgac ctgctgccca atcccccgcc
     1321 gctcatcgag cacggcggtc tggtctccga ggtgactgcc acacccaaga ggcataccac
     1381 gcgctacgtc ggcgtctgcg cctcgatccg cgcggctgcc gccgccgcca tgccggagcg
     1441 cccccagcac gcgctcgaga tcccctctat ggtcgatctg gccgagttcg atcccggcgc
     1501 gcggccggcc gtgcgccgcg cctggggcgt cgatgccaac atgcccgtaa tgggctggat
     1561 cgggcggctg gatcgcaaga agcgagttga ggacttcatt cgcgcggcgg cgctggtgct
     1621 agtccggcat ccacacgccc gcttcgtgat cgtcggcggc cccgacgcct atatgcccga
     1681 gtacgccggc gagctgcggg agctggccgc ccacctggag ctgggtgcgg ccctgtcatt
     1741 cctcggcgat cgaccggacg tgccgcgcct cctctccggt ctcgatgcgt tggtgtggct
     1801 gtcgcgcggt gaggggatgc cgcacgtgat cgcggaggcc ggggcggcgc gcctgccggt
     1861 gatcgccacg cgcgacaacg gctcggagca gcaaatcatc gatggcgtca cgggcctctt
     1921 cgtaccccac gagtcccctt ccgcggtcgc cgaggggatg gaccggctca tcgctgggcc
     1981 agccctgcgg gcgcggttgg gaggcaatct tcgccgcaag gtcgagcgcg agtacgcggc
     2041 ggacatcgtc gtgccgcgtt ggcaggcatt gttcgacgag gtgctggcgc agcgaatctg
     2101 aaacggtgac cccggtctgc ttccggttcg ccccaatgcc gctcgacgac gtcgcgggtg
     2161 aggcgtccgc tactcctcgg ccggcaccac agccggctcg gaggcgaggt ccgcgatcaa
     2221 ctgctccacg atcccctgcc cggcagtctc ggtcgatgcc ttcacggctg gggtggccag
     2281 ccggttgaag agcgcgagcg cctgaccgac cacctgatcc atgttgtagt actggtaggt
     2341 ggcaagtctg ccgacgaagt ggacgccggg cgtgctctcg gccagcgcct tgtagcgcgc
     2401 gtagagctcg cggttggcgg ggcgcggaac cggatagtag ggatcgccgt tcgcctgagg
     2461 gtactcgtac acgagcgtgg ttctggggtg ctcctgcccg gtgaggtgct tgaactccgt
     2521 gaccctggtg tattcgaagt cgttggggta gttgagtacg gccaccggct gctgccggga
     2581 cgagctgtgg ctctcgaagc ggaagctgag ggaacggtag ggcagccggc catagcagta
     2641 gtcgaagtac tcgtcgatcg gaccgctgta gatgacctcg cggtggggaa tgagatcacg
     2701 cacctcgcgg tagtcggtgt tcaggaggat cttgatgttg gggtgcgcga gcatgcgctc
     2761 gaacatccgg gtgaagccgt ggaggggcat gatctggtag cggtcggcga agtagcgatc
     2821 gtcccggcca gtgcgcgtcg gcacgcgggc ggtcaccgtc gcgtccagct cggaagggtc
     2881 gaccccccac tgcttgcgcg tatagccgcg gaagaacttc tcgtagaggt cgcgcccgac
     2941 cttgctgata accacgtctt ccgcgctccg gatcggctct cgtggctccg cgacagactt
     3001 gaagaaggcc tccagatcga acgaggagaa gtgggtgccg tagagacggt tgacggtgtc
     3061 gaggttgatc gggatcggca agagctggcc gtcgacggag gccagcacgc gatgctcgta
     3121 gggccgccat gtcgtgaact gggagaggta ggcgacgatc cgctccgagt tcgtgtggaa
     3181 gatgtgcggg ccgtaccggt gaatcagaat gccgtcgtcg tcgtagcagt cgtaggcgtt
     3241 gccgccgatg tgcggacgcc ggtcgacgac gagaacgccc ttgcccagct gactggccag
     3301 ccgctcggcc agcacgctgc cggcgaagcc cgcccccacc acgaggtagt cgaatccctt
     3361 catcgccccc gcgcctccga tgaccccctc ggaccgtttt cacggccgct gcgttgaccg
     3421 tcgccggcca acggcagcag cgacggtcgc ccagcccgct cgacgacgat ttcgttcacc
     3481 agctctgcca ttcccgtcca ggtgcgatcc caggaagtgc cggcgagcat cgcgtcgatc
     3541 gccgcgaggc gaacctcggc cggctccgtc agggccgctt ccaccgccgt gacgaacgcc
     3601 tcgacggagt ccgcgatccg gaccaatccg agcgccccgt agggctggac cacgtcggga
     3661 atggccgtcg aaaccacggc tttcccggct gccaggtact ccagcgtctt ggttgggctg
     3721 atgaatctgg tggcggcgtt gcgcgcgaac ggcatgagcg cgacatccca ggccgccagg
     3781 tagctcggca acgtgtcgta gggcttctgc cccaggtagt gcaggttgtc acgtcgcggc
     3841 aggctggcgc tgtcgatctt ggcgacgggg ccgacgagca ccaattgcca gcaggggcgc
     3901 cggcgggcga cctcgtcgag gagttcgaga tccatccgct cgtcgatgac gccgaagaaa
     3961 ccgagcttcg gtctcacgag cgcgcgctga tcggccggct cgtccatggc cgcgcgcgcc
     4021 cgtgcgaagt gctccagatc gacgctgctg gggaagcaat agacgcgcgg atgctgctgg
     4081 cgcttggcat cgtacaggct ccggccgccc gtgaaaacga cgtcggcgcg cgcgaagagc
     4141 tcccgctcat agcgtgcgag ccgcgcgggc gcgccgcgaa aagccgccag ctcatccatg
     4201 cagtcataga cggtcgcgag cggccgcaga tggtcgctgt agccaagcgc caccggcgtg
     4261 tagtaccaga gcacctggga gccgatgccc tgctccgcga agagccgatc gaggagctgc
     4321 cgctggagag cttcccaggc agccgggccc gcctccagta ccgactccgg aaggtgtgag
     4381 ctgacgacat gcacaccagg ggccggctcg cggatcacga accgcggctc atcgcctgca
     4441 tagtcgggct cttcgacata gaagacccga tgcccgcgcg cccaacggca cagcaggtgt
     4501 tgcggacgct gatagacgta atcccagcgg agatgggaga aacagacgac gtcgtaccgc
     4561 ggagccatcc ttcctccttg ttgcctcgaa acgcaaacgc cggaccattg gccagtctgc
     4621 cttgtcctgc acgacgatcg agcccccgcg tgctctccgg ccacaccccc gccacgccgc
     4681 gtgctcccgg gggcgcgcct ggcccggaag atgcgccaat gcacgccatc cgacgggcgc
     4741 gagatgcgcc cagactcgtg cgagttcggg aggtgcgtga ctgagcagca ggaagcgcag
     4801 ggcagcggag gcgacgccgc gcgtctccgt gctcatgccc acgttcaacc aggcggcctt
     4861 catccgccgc gcactggaca gtctcttggc ccaggtgctg gaggactggg agctggtgat
     4921 cgtcgacgac ggctcgaccg acgagacggg cagcgtgctg gcgccctacc gggaagaccg
     4981 gcgtgttcgc tatcggcgac tcgagcgcaa ccggggccta ggcgccgcgc tgaacctcgg
     5041 cctgtcgctg gccagggcac cgcttgtcgc ctacctgccg tcggatgacg tctactaccg
     5101 ggatcatctg gcgtccctgg cctgcacgct cgaccgtcac cccaccgccg cgctcgccta
     5161 cgcgggcgtg cgccatttct acaatcgaac cgtggtcggg cggatcgagg gctatccgct
     5221 ccaactggtg caggttatgc accgccgcac ggaggaccgc tggctcgagc gcgacgagct
     5281 cgtcaccgac gatctggacc gtatgtactg gtcgaaactc aggctcaggg gaggcgccca
     5341 ggaaaccggg ctcgtgacct gcgagtgggt ggaccatccg tatcagcgcc acaaggtgct
     5401 gcgtgagccg gtcggcggca tcaaccccta ccgccagcgc tacggcgtca gcagcccgct
     5461 gcgctttcac tcgaccgtcg gcaaccgcat cgacgaggta gaacacttcc ggcgcttccg
     5521 cgagcgaccc cgcacaccgc cggcgcccga cggcctcaag atcctgctcg tcggggagct
     5581 cgcctacaat cccgaacgcg tgctggcgct ggaggagcgc ggccacacgc tctacggcct
     5641 ctggatgcca gacccctact ggtacaacac ggtcgggcct gtcccgttcg gacacgtcgc
     5701 agatctgccg cgcgccggct ggcaggaggc gattcgtcgc gtgcggcccg acgtcatcta
     5761 cgcgctgctg aactggcagg ccgtgccctt cgcccaccat gttctgaccg agaacccggg
     5821 catccccttc gtctggca
//
LOCUS       DASUBI010000530         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_234462, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000530 DASUBI010000000
VERSION     DASUBI010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234462"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFU72_10215"
     CDS             complement(<1..584)
                     /locus_tag="VFU72_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423903.1"
                     /translation="MSGAAGHVALYALLPLSATALGAALALLRPPGPRVRSGLQHFAA
                     GVVFSVASVELLPEIVAAHTPGEVAITFSLGVLLMLGLSAVLPQAAVGEAGAAGRTPL
                     PLTLLAAVGIDVFIDGLLLGIGFALGEHAGRLLALALTLEALSLGLATALVLADTALR
                     RRLAFTIMLGTGLLFLAGGVLGAALLSSVSHHTLS"
     gene            complement(689..1426)
                     /locus_tag="VFU72_10220"
     CDS             complement(689..1426)
                     /locus_tag="VFU72_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5423904.1"
                     /translation="MTHEFEIIGLGSERLGTIGRALVVVAHPDDVESHCGGTVARLAD
                     AGCGVTLVLATSGDKGSADPHAQPAEVAARREAEQRAAAELLGIAEVVFLRWPDGEVV
                     NGKALREQIVRQVRTHRPDLVITHDPEHPWPPYTAHRDHRALGRATLDALYPDARDPL
                     YFPEQLAEGLAPHRTPEAWLIMSQAPDLAVDISAVFARTLAARLAHASQYQDAAALTR
                     NLRARAAAQGSPYGLALAERLKRVAFG"
     gene            complement(1430..2032)
                     /locus_tag="VFU72_10225"
     CDS             complement(1430..2032)
                     /locus_tag="VFU72_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5423905.1"
                     /translation="MALRADAHAAGLLRRGLRLEYATLGWNVVGVAVIAVAAVRAGSV
                     ALAGFGLDSLIEIFASVVVVWELTGAPQERETRALRLIGGAFFALALYILAQTAYILV
                     SGAHPGSSPLGIGWLVVTVLAMWLLAWGKRVTGRALGNPVLRKESYVTLIDGLLAAAV
                     LAGLVLNALFGWWWADPLAGLVIVFYGLREGWAAWQEGTG"
     gene            complement(2494..>3231)
                     /locus_tag="VFU72_10230"
     CDS             complement(2494..>3231)
                     /locus_tag="VFU72_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423906.1"
                     /translation="LLAVVLALPAAARSGMMGLHASSPPATGTTLSLAEARAGFASYL
                     QRQGYTDLKVGELMQFGNNFSAEAVKSDTEAGAMELLMSLDGQALGREPGPAMMWNTA
                     YSPLNRAQGMGFHGMMGSDQGMMGGSHGHGMMGGHDMQGAQATPGSGVNGACDGAGSM
                     GYHADTAARLDQPLTVDAAQARLQAWLDQNRSGVSATDPTAFPGYVTFHTERRGQITG
                     MVSVQATTGAIWEHTWHGDFVAMDTGS"
BASE COUNT          487 a         1194 c         1136 g          414 t
ORIGIN      
        1 gagagcgtgt ggtgcgagac gctgctcaag agcgccgcgc ccagcacgcc gccggccagg
       61 aagaggagcc cggtaccgag catgatggtg aaggcgagac ggcgccgcag ggcggtgtcg
      121 gccagcacca gcgcggtcgc cagtcccagt gagagcgcct ctagcgtcag cgccagggcc
      181 agcagccggc cggcgtgctc ccccagcgcg aagccgatcc ccagtagcag cccgtcgatg
      241 aagacgtcga tgccgaccgc cgccagcagc gtcagcggca gcggcgtccg gccggcggcg
      301 cccgcctcgc cgaccgcggc ctggggcagc accgcgctca gcccgagcat caagagcacg
      361 ccgagcgaga aggtgatggc gacctcgccc ggcgtatgcg ccgccacgat ctccggcagc
      421 agctcgacgg acgccaccga gaagacgacc ccggcggcga agtgctgcag gccgctgcgg
      481 acgcgcgggc cgggcgggcg cagcagcgcc agcgccgccc cgagcgcggt agccgacagc
      541 ggcaggagag cgtagagcgc gacatggccg gcggctccgc tcaccgtatt aattccgttc
      601 cccggcgcct ctggcgccca gtggtctgcg atcggcgtat cgggcaatcg ggccgataag
      661 agcaggggag cgcgcgcgct gagtcgcctc agccaaaggc gacgcgcttg agccgctcgg
      721 ccagtgccag gccgtagggg gagccctggg cggcggcgcg ggcgcgcagg ttgcgcgtca
      781 gcgccgcggc gtcttgatac tggctggcgt gggccagccg ggcggccagc gtgcgcgcga
      841 agaccgcgga gatgtcgacc gccagatcgg gcgcctgaga catgatcagc caggcctcgg
      901 gcgtgcggtg cggcgccaat ccctcggcga gttgctcggg aaagtagagc ggatcgcggg
      961 cgtcggggta gagcgcgtcg agcgtggcgc ggccgagcgc gcggtggtcg cggtgggcgg
     1021 tgtagggggg ccaggggtgc tccgggtcgt gggtgatcac cagatccggg cggtgcgtcc
     1081 gcacctgccg cacgatctgc tcgcgcagcg ctttgccgtt cacgacctcg ccgtccggcc
     1141 agcgcaggaa gacgacctcg gcaatcccca gcagctccgc cgccgctcgc tgctcggcct
     1201 cgcgccgcgc cgcgacctcg gctggttggg catgcggatc ggcgctcccc ttgtcgccgg
     1261 aggtcgccag cacgagcgtc acgccgcagc cggcatccgc cagccgcgcc accgtcccgc
     1321 cgcagtgcga ctcgacatcg tccgggtgcg ccaccactac cagcgcgcgg ccaatcgtgc
     1381 ccagccgctc gctcccaagg ccgatgatct caaactcgtg cgtcatggat cagccggttc
     1441 cctcctgcca ggcggcccag ccctcgcgca gaccatagaa gacgatgacc agcccggcca
     1501 gcggatctgc ccaccaccag ccgaagagcg cgttgagcac gagaccggcc aggaccgcgg
     1561 cggcgagcag gccgtcgatg agggtgacgt aggattcctt gcgcagcacg gggttgccga
     1621 gcgcccgccc ggtgacgcgc ttgccccagg ctagcagcca catcgccagc accgtgacga
     1681 ccagccagcc gatgccgagc ggcgagctgc cggggtgcgc gccgctgacc aggatatagg
     1741 cggtctgggc caggatgtag agcgccagcg cgaagaaggc gccgccgatc agccgcagcg
     1801 cgcgcgtctc gcgttcttga ggcgctccgg ttagctccca gaccaccacg accgaggcga
     1861 agatctcgat cagcgagtcg agcccgaagc ctgccagcgc gaccgagccg gcgcgcaccg
     1921 ccgccactgc gatcaccgcc acgccgacca cgttccaacc gagcgtggcg tactccagcc
     1981 gcagaccgcg ccggagcagg ccggcggcat gcgcatctgc cctcagcgcc attacttccc
     2041 cttctcgcct cgctctgacg cggcttcgga ctgagactga agcggcgcac ccgggctgtc
     2101 caggaggttg gagtcaccga gcgatgcttc gccgcggctc ggcaagccgg ggcaaggcca
     2161 tgccgaggcc gcggctgaag acgcgctctt gccgtccgca tgccgccacc gggcacgcct
     2221 ccgcccgcgc catgctgcca taggcggcgg gagccgggca agcatcgccg gatcatgatc
     2281 cggcgccgga catcgcgctg tgcgggcggc ttgtcgccga acctcggcgc cacgagcggc
     2341 cggcgtcttg cctcagccgc gagcgcaccc cgactccggc ccgccggagc cggggtgcgc
     2401 tgaatccctt gcgcgaacct ccatcaggag ccagcaccgc ggccagaacc agcgccccga
     2461 gatagtgcgc ggaatcccct gcgcgaacct ccatcaggag ccggtgtcca tggcgacgaa
     2521 gtcgccgtgc caggtgtgct cccagatggc gccggtcgtg gcctgcaccg agaccatccc
     2581 ggtgatctgg ccgcgccgct cggtgtggaa ggtgacgtag ccggggaatg ccgtcgggtc
     2641 ggtcgcgctg acgcctgacc ggttctggtc aagccaggcc tgcagccgcg cctgcgccgc
     2701 gtccacggtc agcggctggt cgagccgcgc ggccgtgtca gcgtggtagc ccatcgaccc
     2761 cgcaccgtcg caggctccgt tcaccccgct gcccggcgtc gcctgggcgc cctgcatgtc
     2821 gtggccgccc atcatcccgt ggccatggct accacccatc atcccctgat cggagcccat
     2881 catgccgtgg aaccccatgc cctgagcgcg gttcagcggg ctgtaggcgg tgttccacat
     2941 cattgccggg ccaggctcgc ggccgagcgc ctgaccgtcg aggctcatca gcagctccat
     3001 ggcgccagcc tcggtgtcgc tcttgaccgc ctcggcggag aagttgttgc caaactgcat
     3061 cagctcgccg accttgaggt cggtgtagcc ctgccgctgc aggtagctcg caaacccggc
     3121 gcgcgcctcg gccagcgaca gcgtcgtgcc ggtcgccggc gggctgctcg cgtgcaagcc
     3181 catcatccca cttcgcgccg ccgccggtag cgccagcacc acggccagca a
//
LOCUS       DASUBI010000531         4435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_743853, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000531 DASUBI010000000
VERSION     DASUBI010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743853"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VFU72_10235"
     CDS             <1..1520
                     /locus_tag="VFU72_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5423907.1"
                     /translation="HKVRIMDSALVAAAVLSDRYITNRFLPDKAIDLVDEAAARLRME
                     ITSMPAELDELNRRLIQLEIEREALRKEKDEASRRRLEELERELANLREQHGALHARW
                     EQERSEIGRISELKAMIEETRRQIERAQLSADYARASELQYGELRERERELQEAEKRV
                     AQNEAQGKLLKEEVDAEDIAEVVSKWTHIPVSKLVEGEIEKLVHMEERLHERVIGQDE
                     AVDAVSNAIRRARAGLQDPNRPLGSFIFLGPTGVGKTELARALAEYLFDDERAMVRID
                     MSEYQERHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEKANPEVFNVLL
                     QVLDDGRLTDGHGRTVDFRNTIIIMTSNLGSSYIQAGGSETRRRVMNALQEHFRPEFL
                     NRVDEIVIFHALTRENLSHIVDIQVAQVRRRMGERGVELQVTPRAKEWLAERGYDPVF
                     GARPLKRVIQRELLDPLARELLGGKVQEGETVTVDVGPDGELTFHPHLAEAEVVA"
     gene            1647..1772
                     /locus_tag="VFU72_10240"
     CDS             1647..1772
                     /locus_tag="VFU72_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423908.1"
                     /translation="MHLITDLIELIIIVVVLIVIPILFTLVFDIAAHLRELFGRD"
     gene            1870..2226
                     /locus_tag="VFU72_10245"
     CDS             1870..2226
                     /locus_tag="VFU72_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423909.1"
                     /translation="MAQSPDERAATYRTLLNDNYALVESLPDKEQRIAERVAAGEPIP
                     FVANDESISEGAVWRLLEHLARFIRGEEPVEPSVTGGLGSDSEPGVTGGYGETAMGSL
                     ESDFPLPDPDEPEEPV"
     gene            2283..3971
                     /locus_tag="VFU72_10250"
     CDS             2283..3971
                     /locus_tag="VFU72_10250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEU5423910.1"
                     /translation="MTPSSEALTSLLRRFGHDEFRPGQRRVIDNLLQGRDVLAVLPTG
                     SGKSLTYQLVAQLLPGATVVVSPLIALMQDQIESLEAVAMPAELVNSTRSASEQHVAL
                     ERLRRGDAKLLYVTPERFENAAFMRAMARVPVSLLAVDEAHSISEWGHDFRPAYLNLG
                     AAAAQLGHPPILALTATATPWVRGEIIERLGLRDPVLVVHGTDRPNLFFEVLRVEREG
                     EDRDILRRMFGEVAQDYPKPCRAELVEAMQGSGIIYTRTTAAAEETAAWLNQWGIASD
                     YYHGQRCNGERAEVQDRFMRGELRVICATNAFGLGVDKPDVRFVVHRDVPASVESYYQ
                     ESGRAGRDGGFARCTLIYRPGDLSRAAFLAAGSHITEEKVRALRDALRGHRGIGLPRL
                     ERESGLNDRVLGDLVAALEHAGVLRERNGRIALARADIDVAGVTEQIEQRRESYEQSR
                     VEMMRRYAELADCRRVYLLNYFGEEPGFERCDRCDNELLQGADAWIQVREEEAVAVPF
                     TLGDRVIHPEWGAGVVQRVEEDALTVLFDEAGFKSLSSQLVLENYLLRLADWTA"
     gene            complement(4212..>4435)
                     /locus_tag="VFU72_10255"
     CDS             complement(4212..>4435)
                     /locus_tag="VFU72_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423911.1"
                     /translation="EELNELIGTPRPFPLPIPRPRTGYHWDQPQTNEPQQRHRALRRV
                     GKVTASAAAFALASAAALLREPSRETNQP"
BASE COUNT          730 a         1413 c         1584 g          708 t
ORIGIN      
        1 accacaaggt gcgcatcatg gactcggcgc tggtggccgc ggcggtcctt tcggatcgct
       61 atatcaccaa ccggttcctg cctgacaagg cgatcgacct ggtcgacgag gcggcggcgc
      121 ggctgcgcat ggagatcacc agcatgccgg ccgagctgga cgagctgaac cgccggctga
      181 tccaactgga gatcgagcgc gaggcgctgc gcaaggagaa ggacgaggcc tcgcgccgcc
      241 ggttggagga gctggagcgt gagctggcca acctgcgcga gcagcacggc gcgctgcacg
      301 cccgctggga gcaggagcgc agcgagatcg gccgcatcag cgagcttaag gcgatgatcg
      361 aagagacgcg ccgccagatc gagcgcgccc agctctcggc cgactacgcg cgcgcctcgg
      421 aactgcagta cggcgaactg cgcgagcgcg agcgcgagct gcaggaggcc gagaagcggg
      481 tagcccagaa cgaggcgcag ggcaagctgc tcaaggagga ggtcgacgcc gaggacatcg
      541 ccgaggtggt cagcaagtgg acccacatcc cggtgtcgaa gctggtcgaa ggcgagatcg
      601 agaagctggt ccatatggag gagcggctgc acgagcgggt cattggccag gacgaggcgg
      661 tcgacgcggt cagcaacgcc attcgccgcg cgcgcgccgg cctgcaggac ccgaatcgcc
      721 cgctgggcag cttcatcttc ctcgggccaa ccggcgtcgg caagaccgag ctggcgcgcg
      781 cgctggccga gtatctcttc gacgacgagc gcgccatggt gcgcatcgac atgtcggagt
      841 accaggagcg gcacacggtg gcccgcttga tcggcgcgcc cccgggctac gtcggctacg
      901 aagagggcgg gcagctcacc gaggcggtgc gacgccgacc ctactcggtc gtgctgctgg
      961 acgagatcga aaaggcgaat ccggaggtct tcaacgtcct gctgcaggtg ctggatgatg
     1021 gccgcctgac cgatgggcac gggcgcacgg tcgatttccg caacacgatc atcatcatga
     1081 ccagcaacct cggctcctcg tacattcagg cgggcggctc cgagacgcgg cggcgggtga
     1141 tgaacgccct gcaggagcac ttccggccgg agttcctcaa ccgcgtggac gagattgtga
     1201 tcttccacgc gctgacgcgt gagaatctgt cgcacatcgt cgacattcag gtggcgcaag
     1261 tgcgccggcg tatgggcgag cgtggtgtgg agctccaggt aacgccgcga gccaaggagt
     1321 ggctggccga gcgtggctac gatccggtct tcggcgcgcg cccgctgaag cgcgtgatcc
     1381 agcgcgagct gctcgacccg ctggcgcgcg agctgctcgg aggcaaggtg caggagggtg
     1441 agacggtcac ggtcgacgtc ggccccgacg gcgagctgac gttccacccg catctggccg
     1501 aggccgaagt cgtcgcgtag ttgttgtggg cgttccgcgt cgatccaacg cggaacgccc
     1561 tactcccatt cgcgacgagc cgctgctatg atcgggctag cgatcccggg tggctgcgcg
     1621 gcagcccgcg tcagagaggg tgtccgttgc acctcatcac cgacctgatc gagctgatca
     1681 tcatcgtcgt cgttttgatc gtgatcccga tccttttcac cctcgtcttc gatatcgccg
     1741 cccacctgcg cgagctgttc ggccgcgatt aggcccagcg ctcccggact cctcggttgg
     1801 acctccgcgc gcatccctgc tcctggcacg cggggtgcat tggccctccc cgaggtaagg
     1861 agggcgccgg tggcacagtc gccggacgag cgggcggcga cctaccgcac gctgctgaac
     1921 gacaactacg cgctggtcga gagtctgccg gataaggaac agcgcatcgc cgagcgcgta
     1981 gccgccggcg agcccatccc cttcgtggcc aatgacgagt cgatcagcga gggcgctgtc
     2041 tggcgcctgc tcgagcatct ggctcgcttc atacgaggcg aggagccggt cgagccgtcc
     2101 gtgactggtg gcctcggcag cgattccgag ccgggcgtga ccggtggcta cggcgagacc
     2161 gcgatgggca gcctggaaag cgattttccg ctgcccgatc cggacgagcc ggaggaaccg
     2221 gtctaaccgc gcgcgctgcg ggttttgatc catggggaca aacgagaatg tgaggcgcgt
     2281 gcatgacgcc ttctagtgag gcgctcacgt cactcctgcg ccgctttggg cacgacgagt
     2341 ttcggccggg ccagcggcgg gtgatcgaca atctgctcca gggccgggat gtgctggccg
     2401 tcttgccgac cggctcgggc aagtcgctaa cctaccagct ggtggcgcag ctcttgcccg
     2461 gtgcgacggt cgtggtctcg ccgctgatcg cgctgatgca ggatcagatc gagtcgctgg
     2521 aggcggtggc catgccggcc gagctggtca acagcacgcg ctcggccagc gagcagcacg
     2581 tggccctgga gcggctgagg cgaggcgacg ccaaactcct ctacgttaca cctgagcgtt
     2641 tcgagaacgc ggcctttatg cgggccatgg cgcgggtgcc tgtctcgctc ctggcggtcg
     2701 acgaggcgca ctcgatctcc gagtggggcc acgacttccg acccgcttat ctcaacctgg
     2761 gagccgccgc agcgcaactt gggcacccgc cgatcctggc gctgacggcc accgcgacac
     2821 cctgggtgcg cggcgagatc atcgagcgcc tggggctgcg tgatccggtg ctggtggtcc
     2881 acggcaccga tcggcccaat ctcttcttcg aggtgctgcg agtcgagcgg gagggagaag
     2941 accgcgacat cttgcgccgc atgttcggcg aggtggccca ggactacccc aagccgtgtc
     3001 gtgccgagct ggtcgaggcg atgcagggga gcgggatcat ctacacccgc acgactgccg
     3061 ccgccgagga gaccgccgcc tggctgaatc agtggggtat cgcctctgat tactaccacg
     3121 gccagcgctg caacggcgag cgcgccgagg tacaggaccg cttcatgcgc ggcgagctgc
     3181 gcgtcatctg cgccaccaac gccttcggac tgggggtaga caagccggac gtgcgcttcg
     3241 tcgtgcaccg cgatgtgccg gccagcgtcg agagctatta ccaggagtcg ggccgggccg
     3301 ggcgagacgg cggcttcgcg cgctgcacgc tgatctaccg cccgggcgat ctcagccgcg
     3361 cggcgtttct ggcggccggt tcgcatataa ccgaggagaa ggtgcgcgcg ctacgggatg
     3421 cgttgcgggg ccatcgcggc atcggcctac cgcggctcga gcgtgagagc ggcctcaatg
     3481 accgggtgct gggcgacctg gtcgccgcgc tggagcacgc gggtgtgctg cgcgagcgga
     3541 acggacgcat cgcgttggcg cgggccgata tcgacgtggc cggcgtgacc gagcagatcg
     3601 agcagcgacg cgagtcgtac gagcagagcc gcgtggagat gatgcggcgc tacgctgaac
     3661 tggcggattg ccgccgcgtt tacctgctga actacttcgg cgaggagccg ggcttcgagc
     3721 gctgcgaccg ctgcgacaac gagctgctgc aaggagccga cgcctggatc caggtgcggg
     3781 aggaggaagc agtggcggtc cccttcaccc tgggcgatcg ggtgatccac ccggagtggg
     3841 gcgcgggtgt ggtgcagcga gtggaggagg atgcgctgac cgttctcttc gacgaggctg
     3901 gcttcaaatc gctctccagc cagctcgtgc tggagaacta cctcctgcgc ctggcagatt
     3961 ggacagcgtg acggggcgcg acgttggcct acgacagggc tttcagccgg gtgaggacgg
     4021 atcccttcga caagctcagg atgacaagat gtgggggatt gaggcgtacg agtacgtccc
     4081 gtcgtagtcg tccgcccctt cccctccccc tgtcatcctg agcttgtcga aggttccgtc
     4141 ctcatccgcc acccatacga aaagggcggg ttcgaaaccc gcccctacgt ttgacgtgcg
     4201 aggggagccc cctacggctg gttggtctct cggctgggct cacgcagcag ggctgcggcg
     4261 gaggccagcg cgaatgccgc ggccgatgcg gtcaccttgc cgacgcggcg cagcgcacgg
     4321 tggcgctgtt gcggctcgtt ggtctgcggc tggtcccaat gatagccggt gcgaggccgc
     4381 gggatcggca gcggaaacgg ccgcggcgtg ccgatcaact cgttcaactc ttcgc
//
LOCUS       DASUBI010000532         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1260602, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000532 DASUBI010000000
VERSION     DASUBI010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260602"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VFU72_10260"
     CDS             <1..1733
                     /locus_tag="VFU72_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423912.1"
                     /translation="LLLLVTYRSDELTRRHLLYQLLPLLVRESHAQRLDLHPLDDTGL
                     RALVAPYALPPAEAARLAAYLDERSEGNPFFAGELLRALEEGRALRRASDGWRLGDLA
                     VVGVPPLLRQVLDVRLDRLGEDARDLLAVAAVIGQTVPFAVWQAVAGADEGALLDAAA
                     RATAAHLLTETPDGSGVRFAHALVREALYEGLLLPRRRAWHRRAAEALVAQPHPDPDP
                     IAHHFRRAGDARAADWLVAAGEQAEGAFAWLSAAASSKRAAALLDAAGGAPTRRGWLH
                     FRLASLRQFADPQRSLADLGEAARLADAGGDAALAAGATYQRGKFLYNTGERRRGLEA
                     MASGDRVITALPPPARARLAAGPDAYLASDEERRGSLAEHFAVVGRYAEAGALTGWSS
                     DDPAGDAAAPTGREALALAFAHIALGRPAAARRALGALRRAELRLARHAPLLELVWVA
                     LPYRADHLAERGTLAAEAAAVWTATSGLLPAALPNPARLPLLIVEGGWAEARAHAEAG
                     YAVGAGGTLLVCSRFGALARVQGDAALARTLAHEEFPLGPDTPPGDADYLDALGLLQL
                     AAALALDAGE"
     gene            1752..2192
                     /locus_tag="VFU72_10265"
     CDS             1752..2192
                     /locus_tag="VFU72_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423913.1"
                     /translation="MEAHDRWLAWSGAVFGRAEGALGWAAYHRAAGDPEQARQHAGQA
                     RAYATEPRQPLALLAAHRCLGELATAAGDHAEAATQFDAALTLAVACAAPYERALTLL
                     ALAELRLLSGDTPAAQTLLDEARALLEPLAARPALARADASPCG"
     gene            2183..2410
                     /locus_tag="VFU72_10270"
     CDS             2183..2410
                     /locus_tag="VFU72_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423914.1"
                     /translation="MRLTATPTPAYPAGLSAREVEVLRLVAAGLTNAQVAARLYLSPR
                     TVDQHLRTIYNKIGVDNRTAAAHWAAEHGLT"
BASE COUNT          274 a          976 c          968 g          319 t
ORIGIN      
        1 ccctgctgct gctcgtcacc taccgcagcg acgaactcac ccgccggcac ctgctctacc
       61 aactgttgcc cctgctggtg cgcgagtccc acgcacagcg gctggacctg cacccgctgg
      121 acgataccgg actacgggcg ctggtggccc cctatgcgct cccgcccgcc gaggccgcgc
      181 gcctggcggc ctacctggac gagcggtccg agggcaaccc gttcttcgcg ggcgaacttt
      241 tgcgggcgct ggaggagggc cgggcgctcc gccgggcgag cgacggctgg cggctgggcg
      301 atctggcggt ggtgggcgtg ccgcccttgc tgcggcaggt gctcgacgtg cggctcgacc
      361 gcctgggcga ggacgcgcgc gacctgctcg ccgtggcggc ggtcatcggc cagacggtgc
      421 cgttcgcggt gtggcaggcg gtggccgggg ccgacgaggg cgcgctgctg gacgcagcgg
      481 cgcgggcgac ggcggcgcac ctgctgaccg agacgcccga cggctccggc gtgcgcttcg
      541 cccacgcgct ggtgcgcgag gcgctgtacg aggggctgct cctcccccgc cgccgcgcct
      601 ggcaccgccg cgccgccgag gccctggtcg cccagcccca ccccgacccc gaccccattg
      661 ctcaccactt ccggcgcgcc ggggacgcgc gggcggcgga ctggctggtg gcggcgggcg
      721 agcaggcgga gggtgccttt gcctggctgt cggcggcggc ctcctcgaag cgggccgccg
      781 ccctgctcga cgcggctggg ggggccccga cccgacgcgg ctggctgcac ttccgcctcg
      841 cgtcgctgcg gcagttcgcc gacccgcagc ggagcctcgc cgacctgggc gaggcggcgc
      901 ggctggcgga cgcgggcggg gacgccgcgc tcgccgccgg tgccacctat cagcgcggga
      961 agttcttgta caacacgggc gagcggcggc gcggcctgga ggcgatggcg tcgggcgacc
     1021 gggttatcac ggcgctgccg ccgcccgcgc gggcgcggct ggccgcaggg ccggatgcct
     1081 acctggcgtc ggacgaggaa cggcgcggga gcctggcgga gcacttcgcg gtcgtcgggc
     1141 ggtatgccga ggcgggcgcg ctgacggggt ggtcgtccga cgacccagcg ggggacgccg
     1201 ccgccccgac cgggcgggag gccctcgcgc tggccttcgc gcacatcgcg ctcgggcgcc
     1261 cggccgccgc gaggcgggcg ctcggcgcgc tccgccgggc agagctgcgg ctcgcgcgcc
     1321 atgcccccct gctggagctc gtctgggtgg cgctgcccta ccgtgccgac catctggcgg
     1381 agcgcggcac cctggccgcg gaggccgccg ccgtctggac ggcgaccagc ggtctcttgc
     1441 ccgcggcgct gccgaacccg gcgcggctgc cgctcttgat cgtggagggg ggctgggccg
     1501 aggcccgcgc ccacgccgag gcgggctacg cggtcggggc gggcggcacc ctcctggtct
     1561 gcagccggtt cggcgcgttg gcccgcgtgc agggcgacgc ggccctggcc cggacgctgg
     1621 cgcacgagga gttcccgctc ggcccggaca cgccgcccgg cgacgccgac tacctcgacg
     1681 cgctggggct gctccagttg gctgcggcgc tcgcgctcga cgcgggtgag taggccgagg
     1741 cgcgcgcctg gttggaggcg cacgaccgct ggctggcctg gtccggcgcg gtattcggcc
     1801 gggccgaggg cgcgctcggc tgggccgcct accaccgcgc tgccggcgac cctgaacagg
     1861 cgcggcagca cgccggccag gcgcgggcct atgccacgga gccgcgtcaa ccgctcgcgc
     1921 tcctcgctgc ccaccgctgc ctcggcgaac tggcgacagc ggcgggcgac cacgctgagg
     1981 ccgcaaccca gttcgacgcg gccctcacgc tggccgtggc gtgcgccgcg ccatacgagc
     2041 gggcgctcac gttgctggcc ctggccgaac tgcgcctgct cagtggcgac acgcctgcgg
     2101 cccagaccct gctcgacgag gcgcgcgcac tcctggaacc gctcgccgcc cgcccggccc
     2161 tcgcccgcgc cgacgcctcg ccgtgcggtt gaccgccacg ccgacgcccg cctaccccgc
     2221 tggcctctcc gcccgcgagg tcgaagtctt gcggctggtg gcggcgggcc tgaccaacgc
     2281 gcaggtcgcc gcgcggttgt acctcagtcc gcgcacggtg gaccaacacc tccgcacgat
     2341 ttacaacaaa atcggcgtgg ataatcgcac cgccgccgcc cactgggccg ctgagcacgg
     2401 cctcacgtga ggcggcaggc tggcgcctgg ccgaaatctg cttcgcagat tgtgatctgc
     2461 caacgtaggg tatgacatca gcccagccga ctgccagtcc gcgcagcgga cttcgccaca
     2521 cgcagtgtgt ccaggcc
//
LOCUS       DASUBI010000533         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_970207, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000533 DASUBI010000000
VERSION     DASUBI010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970207"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFU72_10275"
     CDS             complement(<1..385)
                     /locus_tag="VFU72_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5423915.1"
                     /translation="MGTGHLGIAVVGGGLAGLSAARALKQQGYTVDLFERARLLGGKA
                     TSFVVDGVEVDNGQHVFLACCSEFSDFVTRLGPHPDGGPLLYLQPRFDVLLLARGQAS
                     ARLRAFPLPAPFHLAPALLGYRHLSL"
     gene            complement(367..1233)
                     /locus_tag="VFU72_10280"
     CDS             complement(367..1233)
                     /locus_tag="VFU72_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU5423916.1"
                     /translation="MPQAEVRSAGWDLRRSEAYCQALTRREAKNFYWGFIALPRPRRV
                     AIYALYSFARQVDDAVDLEDASGDSLAAHRARLIGCFAGQVDDPVMHVLAEVIRRYGL
                     PREELEGLIDGVALDLRVTRYVTWEELRRYCELVAATIGRMCVRIFGYDDPIALEQAV
                     ELGVAMQLANALRDVREDYQRGRVYLPQEDLARFGLSDHDLASESAPEGWDDLLHFEA
                     ARARQAFAAGLRVREHIPRRAAVCVLTMAGLYLAILDRLEREPELPLRRRLSLDTREK
                     LAVMGRSWARAI"
     gene            complement(1220..2128)
                     /locus_tag="VFU72_10285"
     CDS             complement(1220..2128)
                     /locus_tag="VFU72_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU5423917.1"
                     /translation="MSQLSALQAADAYCRELAARHYENFSVASRILPAHPRLHLARIY
                     AYCRITDDLGDEGGGDALARLAEWRALLLRAFDEPAQPLHPVLLALRPTIRACDLSPE
                     PFLDLIAANVQDQTTTRYEDWDALHGYCIRSAAPVGRLVLRVFGVRQPTAEALSDDVC
                     IGLQLANFAQDVRRDGQRGRVYLLQQVLRREGSAAAVRDLCDRAVRLLASGRELEALV
                     PGRLGVQLALYRLGGEAVVAAIRRGDYRTDLVRPRIAAPAKARILATALLERGRRHAD
                     DWTEPPAGRWAPGPDWDPAAGSDAPG"
BASE COUNT          355 a          829 c          818 g          285 t
ORIGIN      
        1 ctagtgacag gtgacggtag cccagcagcg cgggcgctaa gtggaagggt gccggcagcg
       61 ggaatgcgcg cagccgcgcc gatgcctggc cacgcgccag cagcagcacg tcgaagcgcg
      121 gctggaggta gagcagcggg ccaccgtcgg ggtgcggccc gagccgcgta acgaagtcgc
      181 tgaactcgct gcagcaggct aggaagacat gctggccgtt gtccacctcg acgccgtcga
      241 ccacgaaaga ggtcgccttg ccgccgagca ggcgcgcgcg ctcgaagaga tcgacggtgt
      301 agccctgctg cttgagcgcg cgggccgcgc tcagcccggc cagcccgccg ccgacgaccg
      361 cgatccctag atggcccgtg cccacgagcg ccccatcacc gccagcttct cccgcgtatc
      421 gagcgagagt cgccggcgca gcggcagctc cggctcgcgc tccaaccgat cgaggatcgc
      481 cagatacaac ccggccatgg tcaagacgca gacggcggcg cggcgcggga tgtgctcgcg
      541 cacgcgcagc ccggcggcga acgcctgccg ggcgcgggcc gcctcgaagt ggagcaggtc
      601 gtcccagccc tccggggcag actccgaggc cagatcgtgg tcgctgaggc cgaagcgtgc
      661 caggtcctcc tgcggcaggt agacgcggcc gcgctgatag tcctcgcgca cgtcgcgcag
      721 cgcgttggcc agctgcatgg cgacccccag ctcgaccgcc tgctctagcg cgatcggatc
      781 gtcgtagccg aagatgcgca cgcacatgcg cccgatggtc gcggccacca gctcgcagta
      841 gcggcgcagc tcttcccagg tgacgtagcg cgtcacccgc aggtcgagcg ccactccgtc
      901 gatcaggccc tccagctcct cgcgcggcag cccgtagcgc cggatgacct cggccaggac
      961 gtgcatcacc ggatcatcca cctgaccggc gaagcagccg atcagacggg cacggtgcgc
     1021 ggctagcgag tcgccgctcg cgtcctccag atcgacggcg tcgtcgacct gacgcgcgaa
     1081 gctgtagagg gcgtagatcg cgacgcgccg ggggcgcggc agggcgatga agccccagta
     1141 gaagttcttg gcctcgcgcc tcgtcagcgc ctggcaatag gcttcgctgc ggcgcagatc
     1201 ccagccggcg gagcggacct cagcctgggg catcgctccc ggcagccgga tcccagtcgg
     1261 gtccgggagc ccagcggccg gctggcggct ccgtccaatc atcggcgtgc ctccttccgc
     1321 gctcgagcag ggcggtcgcc aggatgcggg ccttggccgg cgccgcgatc cgcggccgca
     1381 ccagatcggt ccggtagtcg ccgcggcgga tcgccgccac caccgcctca ccgcccagcc
     1441 ggtagagtgc cagttgcacc cccaggcgcc cgggcaccag cgcctccagc tcccggccgg
     1501 aggcgagcag ccgcacggcg cggtcgcaga gatcgcgcac ggcggcggcg ctgccctccc
     1561 ggcgtagcac ctgttgcagg agatagacgc ggccccgctg gccgtcgcgc cgcacgtcct
     1621 gggcgaagtt ggccagctgc agaccgatgc agacgtcgtc cgagagggct tcggcggtcg
     1681 gctgccgcac cccgaagacg cgcagcacca gacgcccgac cggcgcggcc gagcggatgc
     1741 agtagccgtg cagcgcgtcc caatcctcgt agcgggtcgt cgtctgatcc tgcacgttgg
     1801 cggcgatcag atccagaaag ggctccggtg agaggtcgca ggcgcggatg gtggggcgca
     1861 gcgccagcag caccgggtgg agcggctgcg ccggctcgtc gaaggcacgc agcagcagcg
     1921 cgcgccactc cgccaaccgg gcgagggcgt cgccgccgcc ctcatccccc agatcgtcgg
     1981 tgatgcggca gtaggcgtag atgcgcgcca ggtgcagccg cgggtgcgcc ggcaggatgc
     2041 gcgaggcgac tgagaagttc tcgtagtgac gcgcggccag ctcgcggcag taggcgtcgg
     2101 cggcctggag cgcactcaac tggctcactt gccacctcca ccgccacctc cgaccaccga
     2161 tagaagcccc agttcccggt cgaacgcgtc gcgcagcgcc ggctgcgtcg agatgagccg
     2221 gcgcgtgccc tggaggtcgg ataccgtcgg cgtgcccagg cagaacatgg tggtgcgcag
     2281 cgtctcg
//
LOCUS       DASUBI010000534         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1091453, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000534 DASUBI010000000
VERSION     DASUBI010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091453"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFU72_10290"
     CDS             complement(<1..673)
                     /locus_tag="VFU72_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423918.1"
                     /translation="MEGSSDGAANSGGTINSSGAAGSDGAAGVGDDDSQPTVTQTWSQ
                     PPDLMQRSGAPRYDAQDLARMLGVSIATLWNWEQNLGIPRPIPVRDERGGFLPRYSDR
                     DVLAVLWLRDQLRAGASPVGAARRLFQAQRPGGPQQGGRSSGSLASAPTSGPLARGAT
                     SGGLGSPASSSTVRGVAPPTGGIGLGSATSGPLGRPGALQPPPGPGIAAMRFTSGPLT
                     RGPATA"
     gene            1263..>2659
                     /gene="glmS"
                     /locus_tag="VFU72_10295"
     CDS             1263..>2659
                     /gene="glmS"
                     /locus_tag="VFU72_10295"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5423919.1"
                     /translation="MCGIVGYVGPQNATPILLDGLQRLEYRGYDSAGIAVLSAEGALQ
                     VTKRAGKLTNLRAAVALAEPAGTMGIGHTRWATHGRPNDENAHPHADCLGDISVIHNG
                     IIENYAELRDMLAAQGHTFRSETDTEVLAHLIEEHLKTTDDDLFEAVRLALGRVTGSY
                     AVGVVSRMHPATIIGARHNVPLIVGLGEGEQFLASDIPAILRHTRQVVTLDDGEVVRL
                     VPEGIRITDLAGRPVQRAPFHVEWDAEAAEKGGYPHFMLKEIHEQPDAVRRALLGRVI
                     ERPGSHELRLTELDALRASGALARARRVVILACGSSAYAALAGKYAIEQWARLPVEVH
                     IASEFRYADPIVDPDVLCIAITQSGETADTLAATRRAKADGAPVIAITNAVGSAITRV
                     ADAVLYLQAGPEICVVATKSFVTMVTVLYLLGLHLGQLRETLAEAEAQATLAALLALP
                     AQMQGVLERAQREDDP"
BASE COUNT          452 a          925 c          911 g          371 t
ORIGIN      
        1 gagccgtggc cgggccgcgg gtgagcggcc cggacgtaaa ccgcatcgca gctatgccag
       61 ggccaggggg cggctgcagc gctcccggcc ggccgagcgg cccggaagtc gcgctgccca
      121 gcccaatccc gccggtaggc ggcgcgacac cgcgaactgt ggaggaagac gcgggcgacc
      181 cgaggccgcc gctggtggcg ccgcgcgcga gcgggccgga ggtgggtgcg gaagcgagcg
      241 agccggacga ccgccccccc tgctgcggcc caccgggccg ctgagcctgg aacagccggc
      301 gcgccgcccc caccggcgaa gctccggcgc gcagttggtc gcgcagccag aggacggcca
      361 gcacatcgcg atcggaatag cgcgggagga agccgccacg ttcgtcgcgc acgggaatgg
      421 gccgtggaat acccaggttt tgttcccaat tccacagcgt ggcgatgctg accccaagca
      481 tgcgtgccag gtcctgcgcg tcatagcgcg gggcgcccga gcgctgcatc aggtcggggg
      541 gctggctcca ggtctgcgtg accgtcggct ggctatcgtc atcgccaact cccgccgctc
      601 cgtcagatcc agccgctcca ctggagttta ttgtcccacc ggagttggcc gcgccgtcgc
      661 tcgacccctc catcgcctgc gctcctctcc cgcgtttccg ccgctacctt gcagccaggc
      721 acgtggtccc acgcgccact ccgatcctga ctgcgggttg aggcgttccg atggggagga
      781 gcataccacg agcagccggt aaatgcaatg ccaaggacca ggcggtaaag gccctgccgc
      841 gcactcacaa cgcgtgtgcc agatcgtgca ccacgcggcg aagccgcccg cgtcggcctg
      901 ccgcccgatg gtaccgggga actgcggctc gaccagcgcg aaccacgata ccgcatgggc
      961 acctgacgtc gaccagaccg atacaccgag cattgcgctg ggtgctacaa tagcgctggt
     1021 gccgcgcctg cgcgtggcac gttatatgtt acggataccc cgaacaggac aggttcagta
     1081 gcgcacggac tggggcgtga ggaggggccc cagttgacga gggcggaggc ttatcgaatc
     1141 gtcagcggat gcctccaggg cgggcgacgc catcccctac gaagggcgcg agcaaaaccg
     1201 gcgggtgacc gccggcacag aggcgcggcc aatggcggat accataagga gagtgctctc
     1261 tcatgtgcgg catcgtgggg tacgtgggac cccagaacgc cactcccatc ctcctcgacg
     1321 gactgcagcg actggaatat cgtgggtacg attccgcggg catcgccgtg ctctcagcgg
     1381 agggcgcgct gcaggtaacg aaacgagccg gcaagctgac caacctgcgc gcggcggtgg
     1441 ctctggccga gccagccggc accatgggca tcggccacac acggtgggcg acccatggcc
     1501 gcccaaacga cgaaaacgcc cacccccacg ccgattgcct gggggatatc tcggtgatcc
     1561 acaacggcat catcgagaat tacgccgagt tgcgcgacat gttggcggcc cagggtcata
     1621 ccttccgctc cgagaccgac accgaggtgc tcgcgcacct gatcgaggag catctcaaga
     1681 ccaccgatga cgatctgttc gaagcggtac ggctcgcgct ggggcgggtg acgggctcat
     1741 acgccgtcgg cgtcgtcagt cgcatgcacc ccgcgaccat catcggcgcg cgccacaacg
     1801 taccgctgat cgtcgggctg ggcgaaggcg agcagttcct cgcctcagac atcccggcca
     1861 tcctgcggca tacgcggcag gtcgtgacgc tggacgacgg ggaagtggtg cggctggtgc
     1921 ccgaaggcat ccgcatcact gatctggccg gccggccggt gcagcgcgca ccgttccacg
     1981 tggaatggga cgccgaggcg gcggagaagg gcggctatcc gcacttcatg ctcaaggaga
     2041 tccacgagca gcccgatgcc gtgcggcgag cgctcctcgg ccgcgtgatc gagcggccag
     2101 gcagccatga gctacgcctg accgagctag acgctctgcg ggcgagcggc gccttggcgc
     2161 gcgcgcggcg ggtggtcatc ctggcctgcg gcagctccgc ctacgcggcc cttgccggca
     2221 agtacgccat cgagcaatgg gcgcgcctgc cggtcgaggt gcacatcgcc tcggagtttc
     2281 gctacgccga cccgatcgta gaccccgacg tgctctgcat cgccatcacc caaagtggcg
     2341 agacggccga taccctcgcc gccacccgcc gggcaaaggc cgacggcgcc cccgtgatcg
     2401 ccatcaccaa cgccgtcggc agcgccatca cccgcgtcgc cgatgcggtg ctctatctgc
     2461 aggcgggccc ggaaatctgc gtggtggcga ccaagtcctt cgtcacgatg gtcaccgtcc
     2521 tctacctgct cgggctgcac ctcggccagt tgcgcgagac gctggccgaa gccgaggcgc
     2581 aagcgacgct ggcggcgctg ctggcgctgc cagcccagat gcagggcgtg ttggagcgcg
     2641 cgcaacgcga ggatgatcc
//
LOCUS       DASUBI010000535         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1118320, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000535 DASUBI010000000
VERSION     DASUBI010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118320"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFU72_10300"
     CDS             complement(<1..317)
                     /locus_tag="VFU72_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423920.1"
                     /translation="MQLVELEVIAPILGGFDHRSRCQLFRDGAGLGGWLERGDFESYP
                     PALQAEFDALAALLLDLSGRYGRHLVIRVVDPGSLRGRWSSVRFRVRRFPTFIVDDDE
                     QICG"
     gene            639..1811
                     /locus_tag="VFU72_10305"
     CDS             639..1811
                     /locus_tag="VFU72_10305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU5423921.1"
                     /translation="MNDAASGAYTFRLPTEVRFGEGIAFGLGDAVRELGARRVLIVTD
                     PGVRQVGIVDRLIQTLAAPEVAIEIFDAVSPNPRDVECVAGAQRARVFGANLVVGIGG
                     GSPLDAAKAIAGLATNEGPPGAWEAPRAFSNPPLPVIAVPTTAGTGAEVSWSAVITDT
                     DRHLKMTVRDVQLAPRIALVDPELTYSTPRALTAAVGMDTLTHAVEAYTCRLANPISD
                     ALALQAIRLVAANLLRVVQDGNDRPARAAMMQASLLAGMAFRNADVGAVHCLAEAIGG
                     RYDTPHGVANAVFLAGVFAHNVPANPERHAAVAEALGVEPSDHGALRTAGNGAEALRR
                     LAHAAGIPPLSELPGVDPADFPLIAEAAVHNLSNQSNARPLTVEEYERLLRHAWAD"
     gene            complement(1820..2368)
                     /locus_tag="VFU72_10310"
     CDS             complement(1820..2368)
                     /locus_tag="VFU72_10310"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEU5423922.1"
                     /translation="METLPDLLEPNLRLVFVGINPSSYSARAGHYFARRSNRFWPAFS
                     RSRLSLPLRVALGRDALGPEDDRLLPRFGVGFTDVVKRPTANAAELRPADYQRGVPEL
                     LARLARFEPRAVCFHGVTGFRPVLRYGLARTMHEIALGPQPVRLGIIPVFVVPNPSPA
                     NAHFTLEDQIAWYDRLDEWLDS"
     gene            2623..2928
                     /locus_tag="VFU72_10315"
     CDS             2623..2928
                     /locus_tag="VFU72_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423923.1"
                     /translation="MITNTRRWLKRFAISGALAAALAITGATTSLAASNPSGTGQPNQ
                     SCGSATASVEPNGFSTAGFAHAETQYAGSGMSLDHAQSSHAVSQYDVACFQVTQNSH"
     gene            complement(3003..4268)
                     /locus_tag="VFU72_10320"
     CDS             complement(3003..4268)
                     /locus_tag="VFU72_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5423924.1"
                     /translation="MDYQRAFAAVDEQEPRLIERLRQLIAIDTCVPPGENYGTVLDLV
                     EPELQRLGFSTQRVVIPPDKVAQIPLPLSGERVNLVARLNNGKPPLSVYAHMDTVPIE
                     EGWDFDPFGAELKDGRIYGRGIADMKGTIGSLLTALDVMRQQGLEPVWDLNVLLCTDE
                     EIGVYPGLYHLALEGYVTAPMLCMEGSQDPVLRRGSNGSLDVTITIMGRSCHSGANYL
                     GINALEEAVPVMQSLLELKRRVEERRSALPLAPAPGAPDHLRPMFNLDIAHAGVKSNI
                     VPGVFTLVVNRRFIPEERLEDVQAEIQEAVDRGMEQSRALEAEVVFTQNYPSYYQSAT
                     HPGARRLREALKLVQGYSDADFVETGSGGSTDMAFVAQVLGTDQIATVGLGRQRESKA
                     HGANESVRLADAKAHVKELIYYFTAPLEE"
     gene            4412..>4857
                     /locus_tag="VFU72_10325"
     CDS             4412..>4857
                     /locus_tag="VFU72_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5423925.1"
                     /translation="MAATRDPFGARGTISTPGGQTYTIYRLAALGDQLAAGLDRLPYT
                     VKVLLENVLRHAGQEPFSEGNVTALAAWQPNSGQFLEFPYLPARVLLQDLTGVPAVVD
                     LAAMRSAVERMGGEAARINPLAPADLVIDHSVMVDAFGSRFAFRRN"
BASE COUNT          783 a         1710 c         1615 g          749 t
ORIGIN      
        1 ccgcagattt gctcgtcatc gtcgacgatg aaggtgggga agcgacgcac ccgaaagcgc
       61 accgaggacc agcgcccgcg cagcgaaccg ggatcgacca cgcggatgac gagatgccgg
      121 ccgtagcgac cgctcagatc cagcaacagc gcggccaacg cgtcgaactc ggcctgcaac
      181 gccggtggat aggattcaaa gtcgccccgc tccagccagc caccgagccc ggcgccgtcg
      241 cgaaagagct gacagcgtga gcggtgatcg aagccgccca gaatgggggc gatcacttcc
      301 aactcgacca gctgcatgcc gatctccagt gacgcgtgca ccgagcacga cgaacccttc
      361 gccgtgccgt ttcagcgggt gcccagaagc cggtccattc agcgccaccc cgacggcgcc
      421 gcggatcggc tgaagcgttg acgatcagga atcggagata agccggccag agccgcaaat
      481 ccgtggttcc ccgctcgtcg ttttcccaaa ccatcctatt gcgccgcctg aacttcgtca
      541 acctggcaac gcgcgggtcg ctcctcaatt acttctgctt gcggtagggt ttcgtccgaa
      601 tcctcagctt gggatcaaga gagagaggag cagcgcgaat gaacgatgcg gcgagcggtg
      661 cctacacctt ccggctgccg acggaggtcc gctttggcga gggtatcgcc ttcgggctgg
      721 gcgacgccgt gcgcgagctg ggcgcgcgcc gggtcctgat cgtcaccgat cccggcgtgc
      781 gccaggtcgg gatcgtggat cgcctgatcc agacactcgc cgcgcccgag gtggcgatcg
      841 agatcttcga cgcggtcagt ccgaatccac gagacgtgga gtgcgtggcc ggcgcccagc
      901 gggcgcgcgt cttcggcgcc aatctggtcg tcggtatcgg cggcggcagc ccgctcgacg
      961 cggccaaggc gatcgccggc ctcgccacca atgaagggcc accgggtgcg tgggaggcgc
     1021 cgcgcgcctt cagcaacccg ccgctgccgg tcatcgccgt gccgaccacc gccggtaccg
     1081 gcgccgaggt ctcctggtcg gcggtcatca ccgacaccga ccggcacctc aagatgaccg
     1141 tgcgcgacgt gcagctcgcg ccgcgcatcg cgctggtcga ccctgagcta acctactcga
     1201 caccccgcgc gctgaccgcc gcggtcggga tggacacgct gacgcacgcg gtcgaggcct
     1261 acacctgccg gctggccaat ccgatcagcg acgcgctggc gctccaggcg atccgcctgg
     1321 tcgccgccaa cctattgcgc gtggtccagg acggcaatga ccggccggcg cgcgcggcca
     1381 tgatgcaggc cagtctgctg gccggcatgg cgttccgcaa cgccgacgtg ggcgcggtcc
     1441 actgtctggc cgaggccatc ggcgggcgct atgacacgcc gcacggcgtc gccaacgccg
     1501 tcttcctagc cggcgtcttc gcgcacaacg tcccggccaa cccggagcgc cacgccgcgg
     1561 tcgctgaggc gctcggggtt gagccatcgg accacggggc gctccggacc gccggcaatg
     1621 gcgccgaggc gctgcgccgg ctcgcccacg cggccggcat cccaccactg tcggaacttc
     1681 ccggcgtcga tcccgccgac ttcccgttga tcgcggaagc agcggtgcac aacctctcga
     1741 accagagcaa cgcgcgcccg ctgacggttg aggagtacga acggctcttg cggcacgcct
     1801 gggccgactg accgcgctat caggagtcga gccactcgtc caggcggtcg taccaggcaa
     1861 tctggtcttc cagcgtaaag tgagcgttgg ccgggctggg gttcggcacg acgaagacgg
     1921 ggatgatacc cagacgtacc ggctgcggcc cgagcgcgat ctcgtgcatc gtccgcgcta
     1981 gcccgtaacg aaggaccggc cggaagccgg tcacaccgtg gaagcagacg gcgcgcggct
     2041 cgaaccgggc taatcgcgcc aggagctcgg gcaccccgcg ctggtaatcc gccggccgca
     2101 gctcggcggc gttggcggtc ggacgcttga ccacgtcggt gaaaccgacg ccgaagcgcg
     2161 ggagcaggcg gtcgtcctcc gggccaagtg cgtcccggcc cagcgcgacg cgcagcggca
     2221 ggctcaagcg cgagcgcgag aaggccggcc agaagcgatt gctgcgccgc gcgaagtagt
     2281 gcccggcgcg cgccgagtag ctggatgggt tgatcccgac gaagaccagc cgaagattgg
     2341 gttcgaggag atcaggcagc gtctccacct cagcgccctt ccgcaggtcg tttcttcgat
     2401 cagtctagcc gcggtactcg tgcggacggt gcgccagttg cgacacgcca ccggtcgtcc
     2461 gaaccgatct gaggcttcgg ggatatggcg cttcatggcc gggagacatc cggctcgagc
     2521 ggatcgccct gccatccgat gcggccgtcc cgccgtaggg gttgcaacaa tccgggcgac
     2581 gcgggttgct ggacgtcgtg aagaggggac aggatgcttg tcatgatcac gaatacccgt
     2641 cgctggttga agcgcttcgc catctcaggc gctctggccg cggcgctcgc catcaccggt
     2701 gcgacaacct cactggctgc ctcgaacccc agcggcaccg ggcaacccaa ccagtcgtgc
     2761 ggctcagcca ccgccagcgt ggaacccaac ggattctcca ctgccggctt cgctcacgcc
     2821 gagacgcaat acgctgggag cggcatgtcg ctcgaccacg cgcagagcag ccacgcggtc
     2881 agccagtacg acgtggcgtg cttccaggtg acgcagaact cccactgact ctgatccccg
     2941 cgtcttcgcc ttgattgggc tgcccagccc ggccgcggat ggccaggctg ggcaaaagtc
     3001 ggtcactctt cgagcggcgc ggtgaagtag tagatcagct ccttgacgtg ggccttcgcg
     3061 tcggcgagcc gcacgctctc gttggcgccg tgcgccttgc tctcacgctg ccggcccaag
     3121 cccacggtgg cgatctggtc agtgcccagc acctgcgcca cgaatgccat gtcggtcgag
     3181 ccgcccgagc cggtctcgac gaagtcggcg tcgctgtagc cctgcaccag cttcagcgcc
     3241 tcgcgcagcc gccgggcgcc gggatgcgtg gccgactggt agtacgaggg gtagttctgg
     3301 gtgaagacca cttcggcctc caacgcgcgg ctctgctcca tgccgcgatc gaccgcctcc
     3361 tggatctcgg cctgcacgtc ctccagcctc tcctcgggga tgaagcgccg gttgacgacc
     3421 agggtgaaga cgcccggcac gatgttcgac ttgactccgg cgtgcgcgat gtcgaggttg
     3481 aacatcggtc gcaggtggtc gggcgcaccg ggcgccgggg ccagcggcag ggccgagcgg
     3541 cgctcctcca cccgccgctt cagctccagc agcgactgca tcaccggcac cgcctcctcc
     3601 agcgcgttga tgccgaggta gttggcgccg gagtggcaac tgcggcccat gatggtgatg
     3661 gtgacgtcga gcgagccgtt ggagccgcgc cgcagcaccg ggtcctggct cccctccatg
     3721 cagagcatcg gcgcggtgac gtagccctcc agcgccaggt gatagaggcc ggggtagacg
     3781 ccgatctctt cgtcggtgca gaggagcacg ttcagatccc agaccggctc gagaccctgc
     3841 tggcgcatca cgtcgagcgc ggtcagcagc gagccgatcg tgcccttcat gtcggcgata
     3901 ccgcgcccat agatgcggcc gtccttgagc tcagcgccaa agggatcgaa gtcccagccc
     3961 tcctcgatcg gcacggtatc catgtgcgcg tagaccgaga ggggcggctt gccgttattg
     4021 agccgcgcca ccaggttgac ccgctcgccg gagagcggca aggggatctg ggctaccttg
     4081 tccggtggga tcaccacgcg ctgggtgctg aacccaagcc gctgcaactc cggctcgacc
     4141 aaatcgagca ctgtgccgta gttctccccc ggcggcacgc aggtatcgat cgcgatcaac
     4201 tggcgcagcc gctcgatcag gcgcggctct tgctcgtcga cggccgcgaa ggcacgctgg
     4261 tagtccatcg gcttcctctc tgactaacaa acggcgctcg ttctgcacga ttgggccgca
     4321 tggtatcctg acctcgggcg ggcgcgccag tccatcggcc tgcctgcccc accgattgcg
     4381 cgatcttccg atccagttgg aggagggctc catggctgcc acgcgcgacc ccttcggcgc
     4441 ccggggaacg atctcgaccc ccggcggaca gacctacacc atctatcgcc tcgcggcgct
     4501 gggcgatcag ctcgccgccg gcctcgaccg gctcccctac accgtcaagg tgctgctgga
     4561 gaacgtgctg cgccacgccg gccaggagcc gttcagcgag gggaacgtca ccgcgctggc
     4621 agcctggcaa ccgaactccg gccagttcct cgagttcccg tacctcccgg cgcgcgtgct
     4681 cttgcaggac ctgaccggcg tgccggccgt ggtcgacctg gccgcgatgc gctcggcggt
     4741 cgagcgcatg ggcggtgagg cggcccgcat caacccgctc gctccggccg atctggtcat
     4801 cgaccactcg gtgatggtcg acgccttcgg ctcgcgcttc gccttccggc gcaacat
//
LOCUS       DASUBI010000536         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_765513, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000536 DASUBI010000000
VERSION     DASUBI010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765513"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFU72_10330"
     CDS             <1..937
                     /locus_tag="VFU72_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5423926.1"
                     /translation="RSAIRPEYWSYLERFPVHSLLIVPLRVRGHLLGTITLWRDQTSA
                     PLTSDDQTLLAQLADRAALAIDNARLYQAAQQALRYRDEFLSIASHELKTPLTTLKGY
                     AQLLRRVLQRQPENAARLARVVDELQVQMERFEVLVTDLLDVSRIEQGHVELRPEPVE
                     LRELAQQVLARFEQEGLAQPDHRLVLDAPRPIVGRWDAERLDQVLTNLISNAVKYSPE
                     GGDVSVALGVRGGQAEMIVTDQGIGIPKDELRELFKPFMRGAAARIFPGTGLGLYISR
                     NLVELHGGTIEATSRAGQGSRFTVRLPLGEAADAE"
     gene            complement(968..2164)
                     /locus_tag="VFU72_10335"
     CDS             complement(968..2164)
                     /locus_tag="VFU72_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU5423927.1"
                     /translation="MKITDLKCALIGANPVVRIATDQGLSGYGEVESYKPYLKPHVLF
                     YRDALLGEDPTDVERVLRKIRRHGAFKPWGSAVSAIEMALWDLAGKAAGLPVHKLLGG
                     KVRDRVRVYNGGVRFQMFSHTPEGFAENMARMRAAPEGFTIIKESIGFHGNMPSRVPD
                     FFYGEARPAAFHGVHDAGLLTERGFQHILDCVAAMKEVLGDEVGLALDCGPGCTVPDA
                     IRLARALEPLHILWLEDLITGDYVPFTSAASYREVTRATSTPIHTGEQIYLRQNFTDL
                     IEGQAVRVIGPDPCDIGGIAELKWVAEYADIHGILIAPHGIGNGLLGLAALVQVCATL
                     PDNYIAFEYPIGEPAWWYDIVEGLPDPIVAHGFIEVWDRPGMGVDLRVEEARRYLRAE
                     DADFFA"
     gene            complement(2193..3056)
                     /locus_tag="VFU72_10340"
     CDS             complement(2193..3056)
                     /locus_tag="VFU72_10340"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5423928.1"
                     /translation="MEPELVADFDCVTGENPLWHPGEQRLYWIDIGTCRLFRYDPADG
                     HAEQVYQAPEAIGGFTVQADGALLLFMAHGAIHTWREGAVTVIRDELPEARDSRFNDV
                     QADPEGRVFAGVMPSQDHPGMLYRLDRDGTLTKLLDGLGIPNGMGFTPDLRQLYFTDS
                     TSRTIYLFDYDRASGALRHQRVFVQLPRDSPEQPDGLTTDTAGGVWSARWDGSALYRF
                     APDGTVAARVGFPARKVSCPAFGGRDYTDLYVTTAGGNHKATEGQGAGALFRLRPGLR
                     GMADFPSRVGI"
     gene            complement(3066..>3409)
                     /locus_tag="VFU72_10345"
     CDS             complement(3066..>3409)
                     /locus_tag="VFU72_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423929.1"
                     /translation="AITVGDVFYLIPPTGQRQLIARCFALLAPGGRLLWKSQVRRPRW
                     KFAITYAQEWLMTHLGPTRGSGLFFLDTGESLAALRRAGFQATGQPLTSWRPYTDVLF
                     VAVKPSEQRSE"
BASE COUNT          515 a         1138 c         1242 g          514 t
ORIGIN      
        1 ccgctccgct attcgccccg agtactggtc ctatctggag cggttcccgg tgcacagcct
       61 gctgatcgtc ccgctgcggg tgcgcggtca cctgctcggg acgatcacgc tctggcgcga
      121 ccagaccagc gcgccgctga cgagcgacga tcagacactg ctggcgcagt tggccgaccg
      181 ggccgcgctg gcgatcgaca acgcccggct ctatcaggcc gcgcagcagg cgctgcgcta
      241 ccgcgacgag tttctctcga tcgcctcgca cgagctgaag acgccgctga ccacgctcaa
      301 gggctacgcg cagctcctgc ggcgcgtgct gcagcgccag cccgagaatg ccgcgcgact
      361 ggcgcgcgtg gtcgacgagc tgcaggtgca gatggagcgc ttcgaggtgc tggtcaccga
      421 cctgctcgac gtgtcgcgca tcgagcaggg acacgtcgag ctgcgcccgg agccggtcga
      481 gctgcgcgag ctggcgcagc aggtgctggc gcgcttcgag caggaaggtc tggcgcagcc
      541 ggaccaccgg ctggtgctcg acgcgccgcg gccgatcgtc ggtcgctggg atgccgagcg
      601 gctcgatcag gtgctgacca acttgatctc caacgcggtc aagtactcgc cggagggtgg
      661 ggatgtcagc gtcgcgctcg gcgtacgcgg cgggcaggcc gagatgatcg tgacggacca
      721 gggcatcggg atccccaagg acgagctacg cgagctcttc aagccgttca tgcgcggcgc
      781 cgcggcccgg atcttcccgg gcaccgggct ggggctgtac attagccgca atctggtcga
      841 gctgcacggc ggcacgatcg aggcgacgag ccgcgccggg caggggagcc gcttcaccgt
      901 gcgcctgccg ctgggcgagg cggcggacgc ggagtgagcg caccggttcc gatcggtcgg
      961 cgcggtctta ggcgaagaag tccgcgtcct cggcgcgcag atagcgtctg gcttcctcga
     1021 cccgcagatc gacgcccatg cccggccgat cccagacttc gatgaagccg tgcgcgacga
     1081 tgggatcggg caggccctcg acgatgtcgt accaccacgc cggctcgccg atgggatatt
     1141 cgaaggcgat gtagttgtcg ggcagcgtgg cgcagacctg caccagcgcg gccaacccga
     1201 gcagcccgtt gccgatgccg tgcggcgcga tcaggatgcc gtggatatcg gcgtactcgg
     1261 cgacccactt cagctcggcg atgccgccga tgtcgcaggg gtccgggccg atcacgcgca
     1321 cggcctgccc ctcgatcagg tcggtgaagt tctgccgcag gtagatctgc tcgccggtgt
     1381 ggatcggcgt gctggtagcg cgcgtcacct cgcgatagct ggcggcggag gtgaagggca
     1441 cgtagtcgcc ggtgatcaga tcctccagcc agaggatgtg cagcggctcc agcgcccgcg
     1501 ccagccggat cgcgtccggc acggtgcagc cggggccgca gtcgagcgcc aggccgacct
     1561 catcgcccag cacctccttc atggccgcga cgcagtcgag tatgtgctgg aagccgcgct
     1621 cggtcagcag gccggcgtcg tggacgccgt ggaaggccgc cggccgggct tcgccataga
     1681 agaaatccgg cacccggctg ggcatgttgc cgtggaagcc gatactctcc ttgatgatcg
     1741 tgaacccctc gggcgcggcc cgcatgcggg ccatgttctc ggcgaagccc tccggcgtgt
     1801 ggctgaacat ctgaaagcgc accccgccgt tgtagacgcg cacgcggtcg cgcaccttgc
     1861 cgccgagcag cttatggacc ggcagccccg cggccttgcc ggccagatcc cagagggcca
     1921 tctcgatcgc gctgaccgcg ctgccccagg gcttgaaggc gccgtggcgg cggatcttgc
     1981 gcagcacgcg ctccacgtcg gtcggatcct caccgagcag cgcgtcgcgg tagaagagga
     2041 cgtgcggctt gagatagggc ttgtaggact cgacctcgcc gtagccgctg agcccctggt
     2101 cggtggcgat acgcaccacc gggttggccc cgataagcgc gcacttcaga tcggtgatct
     2161 tcaccagtcc tcctcacccg gtacgcaatg ctctagatgc cgacgcgcga gggaaaatcc
     2221 gccatgccgc gcaggccggg ccgcaggcgg aagagcgcgc ccgcgccctg gccctcggtc
     2281 gccttgtggt tgccgcccgc cgtggtgacg tagagatcgg tgtagtcgcg cccgccgaag
     2341 gccgggcacg agaccttgcg cgccgggaag ccgacgcgcg cggccaccgt gccgtcgggc
     2401 gcgaagcggt agagcgcgct gccgtcccag cgagcggacc agacgccgcc cgcggtgtcg
     2461 gtcgtcaggc cgtccggctg ctcggggctg tcgcgcggca gttgcacgaa gacgcgctgg
     2521 tgacgcagcg cgccgctagc gcggtcgtag tcgaagagat agatggtgcg gctggtggag
     2581 tcggtgaagt agagctggcg caggtcgggc gtgaagccca tcccgttggg gatgccgagg
     2641 ccgtcgagca gcttcgtcag cgtgccgtcg cggtcgagcc ggtagagcat gccggggtgg
     2701 tcctggctgg gcatgacccc ggcgaagacg cgcccctcgg ggtcggcctg cacgtcgttg
     2761 aagcgcgagt cgcgcgcctc gggcagctcg tcgcggatga ccgtgaccgc gccctcgcgc
     2821 caggtgtgga tggcgccgtg cgccatgaag aggagcagcg cgccgtcggc ctggacggtg
     2881 aagccgccga tcgcctccgg cgcctggtag acctgctcgg cgtggccgtc ggccgggtcg
     2941 tagcggaaga ggcggcaggt gccgatatcg atccagtaga gccgctgctc gcccggatgc
     3001 cagagcgggt tctcgccggt gacgcagtcg aagtcggcaa ccagttcggg ttccatgatc
     3061 tcacctcatt ccgatctctg ctcgctcggc ttgacggcga cgaagagcac gtcggtgtag
     3121 gggcgccagg acgtgagcgg ctggcccgtc gcctggaaac cggcccggcg cagcgccgcg
     3181 agcgattcgc cggtgtcgag aaagaagagg ccgcttcctc tggtcgggcc gagatgggtc
     3241 atcagccact cctgcgcata ggtgatggcg aacttccagc gtgggcggcg cacctggctc
     3301 ttccagagca ggcgcccgcc gggcgccagc agcgcgaagc agcgggcgat cagctggcgc
     3361 tgcccggtcg gggggatcag atagaagacg tcgccgaccg taatcgcgt
//
LOCUS       DASUBI010000537         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_192044, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000537 DASUBI010000000
VERSION     DASUBI010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192044"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..>2103)
                     /locus_tag="VFU72_10350"
     CDS             complement(311..>2103)
                     /locus_tag="VFU72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5423930.1"
                     /translation="MRLLEREQAARAEAELAAHMIERIQAVSDAALAHLDLDDLLVEL
                     LHRVSELMGVDEATILLLDESGQRLVVRAAEGLAVPPDLRVGVPLGEGFVGRIARNRA
                     PVVIEQVNQEEVPHPFLVERIASMMGVPLVLEERVIGVLHVGSTRPRLFRPSELRLLQ
                     FAADRIALAIDRASLFEAERLAQRRLAFLAEASSVLSRTLEVEEALIRVSELAVPEMA
                     DVCTVNLVEEDGFFRRLSVAHHDPALDRLAEIAVGPYAVDPHDPKVSGMRVFRTGQSL
                     LFPEIAPDDLGEIIGDQRQAELLRETPFCSGMMVPLVTGGRAIGMMGFWLTSRKRIYT
                     PNDVALAEDLARRAAEAYERARLYRETVAAVRAREEFLSIASHELKTPLTTIKGYVQM
                     LDRQLRRPVPEANRLRQAMDNLQGQLERFQGLVNDLLDMSQIQRGRLELHPSSFDLVV
                     LASEILERFEHASERTDEHRLVLEAPEPVLGEWDASRLDQVLTNLISNALKYSPGGGE
                     VRVTVRQVGERAEVIVADQGIGITAEERNTLFQPFSRGAEATRTASGAGLGLHISAQI
                     VRRHDGTIDVESQPGAGSRFRVTLPLTYAP"
BASE COUNT          334 a          746 c          706 g          317 t
ORIGIN      
        1 ctgcgcaccg tcagccccgg ccgcacctga tcgccgattg tcatgatccg tctccttccg
       61 gcacatggcc tcagggcggc ggttgcaagc gacatgccga aggacggaga gcggaggtgg
      121 cgaacgacga ggatgagcgc cgggtgagga tggatccctc cacttcaatc gggatgacat
      181 ggtaagtgag gagtctgacg aagaggacga tcgccttcgt ccgcccctta ctcccccctg
      241 tcatcccggc cgaagtggag ggatccgcca tggagcggca ggcgtcttgt cgatggccac
      301 caatgaagcg ctacggagcg tacgtcagcg gcagcgtgac gcggaagcgg ctgccggcgc
      361 cgggttggct ctcgacgtcg atggtgccgt cgtggcgccg cacgatctgc gcgctgatgt
      421 gcaggcccag gccggcgccg ctggccgtgc gcgtcgcctc ggcgccgcgg ctgaagggct
      481 ggaagagcgt atttcgttcc tcggcggtga tcccgatgcc ctggtcggcc acgatcacct
      541 cggcccgctc gcccacctgg cgcaccgtca cccgcacctc gccgcccccc ggcgagtact
      601 tcagcgcgtt cgagatcaga ttggtcagca cctggtccag gcgcgaggcg tcccactccc
      661 ccagcaccgg ctccggcgcc tccagcacca gccggtgctc gtccgtgcgc tcgctggcgt
      721 gctcgaagcg ttccagaatc tcgctcgcga gcacgacgag atcgaagctc gatgggtgca
      781 gctcgagccg gccacgctga atctgcgaca tgtcgagcag gtcgttgacc agcccctgga
      841 agcgctcgag ctggccctgg agattgtcca tcgcctgccg cagccggttc gcctcaggaa
      901 cgggccggcg caactgccgg tcgagcatct ggacgtagcc cttgatcgtg gtcagcggcg
      961 tcttcagctc gtgggaggcg atcgagagga attcttcgcg cgcacgcacc gccgccaccg
     1021 tctcgcggta gaggcgcgcc cgctcgtagg cctcggccgc gcgccgggcc agatcctcgg
     1081 ccagcgcgac atcgttcggc gtatagatcc gcttccggct cgtcagccag aagcccatca
     1141 tgccgatcgc ccggccgccc gtcaccagcg gcaccatcat gcccgaacag aagggcgtct
     1201 cgcgcagtag ctcggcctgt cgctggtcgc cgatgatttc tcccaggtcg tcgggcgcga
     1261 tttcggggaa gaggagcgac tggccggtgc ggaagacgcg catgccgctg actttggggt
     1321 catggggatc gacggcgtag ggaccgacgg cgatctcggc caggcgatcg agcgccggat
     1381 cgtggtgcgc caccgagagc cgccggaaga agccgtcttc ctcgaccaga ttgaccgtgc
     1441 agacatcggc catctccggc accgccagct cgctgacccg gatcagggcc tcctcgacct
     1501 ccagcgtgcg cgagaggacg ctgctcgcct cggccaggaa ggcgaggcgg cgctgggcca
     1561 gccgctcggc ctcgaacagg ctggcgcggt cgatcgccag cgcgatacgg tcggcggcga
     1621 attgcagcag ccgtagctcg ctgggccgga agaggcgcgg gcgggtgctg ccgacgtgca
     1681 gcacgccgat cacgcgctct tcgagcacca gcggcacgcc catcatcgag gcgatccgct
     1741 cgaccaggaa gggatggggc acctcttcct ggttgacctg ctcgatcacc accggtgccc
     1801 ggttgcgcgc gatccgcccc acgaacccct cgcccagcgg cacgccgacc cgcagatcgg
     1861 gcggcacggc cagcccctcg gccgcgcgca ccaccagccg ctggcccgac tcgtccaaca
     1921 ggagaatagt cgcctcatcg acacccatca gctcggagac gcgatgcagc agctcaacca
     1981 gcagatcgtc gagatcgagg tgggccagcg cggcatcgct gaccgcctgg atgcgctcga
     2041 tcatgtgcgc ggcgagctcc gcctcggcgc gcgccgcctg ctcgcgctcc aacaggcgca
     2101 tac
//
LOCUS       DASUBI010000538         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_269063, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000538 DASUBI010000000
VERSION     DASUBI010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269063"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFU72_10355"
     CDS             complement(<1..802)
                     /locus_tag="VFU72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423931.1"
                     /translation="MLSTAVARLDEWAPAGDLRRLVAVRGLRSLVQGYVMVVFTIYLS
                     QIGFPAWLIGLTLTIVGIASSLLTLVIGVSSDRLGRRPFLLIYSFLLFASGIIFSLTT
                     IPWILIGISALGGMGRGGGGGGQAGAFAPAEQALISEKASGDTRRRVFAANSVVGTSM
                     AAVGALLAGVPEWLRAGSHLSLLASYRPLFLGVALAGFLSMILLWPISDVNRPRQPRQ
                     QSAAARAQRRQTRQRIGQISLAGAVNGFGLGFMAGLVPYWMHIRYGVSP"
     gene            complement(1224..>2474)
                     /locus_tag="VFU72_10360"
     CDS             complement(1224..>2474)
                     /locus_tag="VFU72_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEU5423932.1"
                     /translation="AVTRVSVDDVALNWDIITRTDHSFSHRIDDWSVTAQKQTGRCWM
                     FAGLNTLRFGAKQKMGLKEFEFSQNYLFFWDKFERSNYFLEAIIETADRDVDDRTVAF
                     LLARPLEDGGQWNMFANLIKKHGVVPKVVMPETESSSNSRRLNSIVKGKLRDGAHAIR
                     ALQQQGAGIEAMRAAKDETLATVYRILAIHHGTPPTRFSWEWTDRDGQFHRAPDLTPQ
                     EFAHDYVTMPVDDYVCLVNDPRPSSPYGRTYTVEYLGNVVGGDIVKYLNIEIGLMKEI
                     TQHTIMGGEPVWFGCDCGKMMRRDLGIWDKNLYDFESVYDTDFELSKADRLLYHETAM
                     NHAMVFTGVDVVEGQPQRWRVENSWGPDMADKGFYTMNDSWFDEHMFEIAARKSLLPA
                     ALQEALDLEPVVLPAWDPMGALAR"
BASE COUNT          405 a          818 c          869 g          382 t
ORIGIN      
        1 ccggcgagac gccgtagcgg atatgcatcc agtagggcac cagcccggcc atgaagccca
       61 ggccgaagcc gttgaccgcg ccggccagcg agatctggcc gatgcgctgg cgcgtctgcc
      121 gccgctgggc gcgcgccgcc gcgctctgct gccgcggctg cctgggccgg ttcacgtcgc
      181 tgatcggcca gaggaggatc atcgagagga agccggccag cgccaccccc aggaagagcg
      241 ggcgatagga tgccagcagc gagaggtgcg agccggcgcg cagccactcg ggcacgccgg
      301 ccagcagcgc gccgaccgcc gccatcgagg tgccgaccac gctgttggcg gcgaagacgc
      361 gccgccgcgt gtcgccggag gccttctccg agatcagcgc ctgctcggcg ggagcgaagg
      421 cgcccgcctg cccgccgcct ccgccgccgc gccccatgcc gcccagcgcg ctgatgccga
      481 tcaggatcca ggggatggtg gtcagactga agatgatgcc ggaggcgaag agcaggaagc
      541 tatagatcaa gaggaacggc cgccggccca gccggtcgct gctgacgccg atgaccaggg
      601 tcagcaggga ggaggcgatg ccgacgatcg tcagcgtgag gccgatcagc caggccggga
      661 agccgatctg gctgaggtag atggtgaaga cgaccatcac gtagccctgc accaggctgc
      721 gcaggccgcg cacggcgacc aggcggcgca gatcccccgc cggcgcccac tcatccagcc
      781 gcgcgaccgc ggtcgacagc acgcacaccc ccacacgcga ggcgaggttg aatcaacagc
      841 ctcgctcgac gatatattga tgcgcaatat acttcacgag gtcggcggga acaacggtct
      901 ccgaccggta atagcgctga gccggggaag ctcgtcgtca tctagcgccg ccggcgaccg
      961 gccagcgcgc ggtgaggctg gagagcaaca gacaaggagt gccaggcgag ccagttgggc
     1021 gccgcgtcaa gacggatccc tcgactgcgc tcgggatgac cggggtaatt gggggcgggc
     1081 gacgcggacg tgcctcgtcc ggtaagcccc tccctctccc ggtcatcccg agcctgtcga
     1141 ggaatccgtg ctgccccacg cccatccttc tcgataggca acgcccctct cctccgcagg
     1201 ggagaggggt cgggggtgcg ggcctaccgc gccagggcgc ccatcggatc ccaggcgggg
     1261 agcacgaccg gctccagatc cagcgcctcc tgcagcgccg ccggcagcag gctcttgcgc
     1321 gccgcgatct cgaacatgtg ctcgtcgaac cacgagtcgt tcatggtgta gaagcccttg
     1381 tcggccatgt ccgggcccca gctattctcg acgcgccagc gctgcggctg cccctcgacc
     1441 acgtcgacgc cggtgaagac catggcgtgg ttcatcgccg tctcgtggta gagaagccgg
     1501 tcggccttgc tcagctcgaa gtcggtgtcg tagaccgact cgaagtcgta gaggttcttg
     1561 tcccagatgc cgaggtcgcg ccgcatcatc ttgccgcagt cgcagccgaa ccagaccggc
     1621 tcgccgccca tgatggtgtg ctgcgtgatc tccttcatca ggccaatctc gatgttgagg
     1681 tacttgacga tgtcgccgcc gaccacgttg cccaggtact cgaccgtgta ggtgcggccg
     1741 taggggctgc tcgggcgcgg gtcgttgacc aggcagacgt agtcgtccac cggcatggtg
     1801 acgtagtcgt gggcgaactc ctgcggcgtc aggtccgggg cgcggtggaa ctggccgtca
     1861 cggtcggtcc actcccagct gaagcgggtg ggcggcgtgc cgtggtgaat cgccaggatg
     1921 cggtagacag tggccagcgt ctcatccttg gccgcgcgca tggcctcgat gccggcgccc
     1981 tgctgctgca gcgcgcggat ggcgtgggcg ccgtcgcgca gcttgccctt gacgatcgag
     2041 ttgagccggc ggctgttgga ggagctctcg gtctcgggca tgacgacctt cggcaccacg
     2101 ccgtgcttct tgatcaggtt ggcgaacatg ttccactggc cgccgtcctc gagcgggcgg
     2161 gccagcagga aggcgaccgt gcggtcgtcg acgtcgcggt cggcggtctc gatgatcgcc
     2221 tccaggaagt agttggagcg ctcgaacttg tcccaaaaga agaggtagtt ctggctgaac
     2281 tcgaactcct tgaggcccat cttctgcttg gcgccgaagc gcagcgtgtt cagcccggcg
     2341 aacatccagc agcggccggt ctgcttctgc gcggtgacgc tccagtcgtc gatgcggtgc
     2401 gagaaggagt ggtcggtgcg ggtgatgatg tcccagttga gcgccacgtc gtcgaccgag
     2461 acgcgggtga cggc
//
LOCUS       DASUBI010000539         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_572212, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000539 DASUBI010000000
VERSION     DASUBI010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572212"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFU72_10365"
     CDS             complement(<1..688)
                     /locus_tag="VFU72_10365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5423933.1"
                     /translation="MEFGAHIPVVIGAGERPRLRDFTAYALAAERLGYRLLGANDHLA
                     SRTGWLDGPSILTAAAAVTERILLATTILLPALRHPVVVAKTYGTLDSLTGGRVVVGV
                     GAGVLPADFALAGIPLEERWGRLDESIAVMRALWREPAPVFNGRYYRLDGVGLQPLPA
                     RPGGPPIWVGSWGSPAGLRRAARLGDGWITSGFNTTPERFARDWDFVQGELARLGRDS
                     SPFANALSSLF"
     gene            1046..1288
                     /locus_tag="VFU72_10370"
     CDS             1046..1288
                     /locus_tag="VFU72_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU5423934.1"
                     /translation="MSRRDKLIARILARPPQADFDDVRAVLEEFGWVLARERGSHCTF
                     AKPGEYPIIVPKVGGRKVKRVYLDMICDRLGLDDYS"
     gene            1285..1731
                     /locus_tag="VFU72_10375"
     CDS             1285..1731
                     /locus_tag="VFU72_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEU5423935.1"
                     /translation="MSTTQRKPLETYLSLEYPFEVRVDPDGGYVVAFPDLPGCMTQVE
                     SLDEVGPMAEEIRTLWIETAYDQGLEIPTPSCREAYSGKFNVRLPRSLHRFLAESAER
                     EGVSLNQYVLMLLSQRDAEARIASRLAALEAQLEAIHVGNRSRSAA"
     gene            1892..2998
                     /locus_tag="VFU72_10380"
     CDS             1892..2998
                     /locus_tag="VFU72_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU5423936.1"
                     /translation="MFEGEIVGRPLAEVDTPALVIDADALERNIARMAEFFQGKHAKL
                     RPHAKTHKSPVIAQMQLAAGAVGITCAKLGEAEVLAAGGVRDILIANQIVGERKIARL
                     VGLARHANVAVAVDDFANAAAISEAALAAGSTVRALVEVNIGMNRCGVEPGAPAVELA
                     QRVASLPGLRFFGLQGYEGHLVTVPEHAEREQRVRAAMAPLIETRRAIEAAGIPVTAV
                     SGGGTGTFDITSTIDGFDEIQAGSYVFMDASYGKVEGPGAAFEPALFLWSTIISRPAP
                     ERAVADIGLKTVTSDHGLPVLAGMQGAEVLSLSEEHAKLHVEGEARGLRAGDKVRFQP
                     GHVCTTVNLHDAYVVARGGIVEAVWPVAARGRSR"
     gene            3202..3774
                     /locus_tag="VFU72_10385"
     CDS             3202..3774
                     /locus_tag="VFU72_10385"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU5423937.1"
                     /translation="MSTAKEATRLVTAEELWALPEQPGVRYELAEGELVEVPGAGALH
                     NLIAALVYRLIYAAARGQNLGLVFTDGVGYILRRAPDVLRIPDVSFVARERIPAGGIP
                     EGFWPGAPDLAVEIVSPNDLAQEINDKVHQYLEVGTRMVWVLWPNRRTVTVYLPEGQL
                     RDLGPNDELDGGEVLPGFSVRVASLFEVAQ"
     gene            3911..4567
                     /locus_tag="VFU72_10390"
     CDS             3911..4567
                     /locus_tag="VFU72_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423938.1"
                     /translation="MRRTAEAAAVETVFLICFVFGAAFALLSLLLGFAGHTWHVEHLS
                     HVHLHHDVQVHAGGHDGQLHVDQGEAHLPIFNVSALLAFLTWFGGAGYLLARLSGWPL
                     LLVLAAAVVVGLAGAALIGLILGKILAGERVMNPRDYQLEGTLARVTVSIPAGGVGEI
                     IFSKAGSRRGEAARSVDGRAIARDTEVVVIDYAGGVALVESWEQLVSRPERVPLAGDR
                     "
     gene            4581..6071
                     /locus_tag="VFU72_10395"
     CDS             4581..6071
                     /locus_tag="VFU72_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5423939.1"
                     /translation="MGVIIAIVVVAILTIIVITAGIGSIYRRVSPNRALIVFGSGGTA
                     IVTGGGKLVWPLFQSYQELSLELMSFDVAPSQDLYTAQGVAVSVEAVAQIKVKSDPES
                     IRTAAEQFLTKTQLEREELIRLVMEGHLRGIVGLLTVEQIVKEPEMVAGRVRETVADD
                     LSKMGLEVVSFTIKRVTDEKDYITNMGRPDVARIKREADIAQAEAERDTAIKRAMAAR
                     ESAIAQAQADQERVIAETASQTKQAEATRDLELKRAEYDAAVRRERAIAEKAQDIAAN
                     QAQQQVVAEQVKVEQVEKQGLIQVQELEVQRRERELEATVIKQAAAEQRKIELLAEAE
                     RERLIRQATGQSEATRLQGTAEAEIVRAKGQAEADAMHLRAEAYQEYSQAAMLDKLLG
                     SLPQLAQAFANSLASVDKVTILSTGDGRNGGGGMSGYTGELAKMVAQVPELFETLTGM
                     KMSETIGRLQGLQPTKPPKPAAPTVPANGHAAPVPPAEVGPGEKEE"
BASE COUNT          994 a         2034 c         2151 g          914 t
ORIGIN      
        1 agaagaggga cgagagcgcg ttggcgaagg gcgacgaatc gcgccccaac cgcgccagct
       61 ccccctgcac gaagtcccaa tcgcgggcga agcgctcggg cgtggtgttg aagccggagg
      121 tgatccagcc gtcgcccagc cgcgccgccc ggcgtagccc ggccggggag ccccagctgc
      181 cgacccagat cggcggaccg ccggggcgag ccgggagcgg ctgcaggccc acgccgtcga
      241 ggcggtagta gcggccgttg aagaccggcg ccggctcgcg ccagagggcg cgcatgaccg
      301 cgatgctctc gtcgagccgc ccccagcgct cctccagcgg gataccggcc agcgcgaagt
      361 ccgccggcag cacccccgcg ccaacgccga ccaccactcg cccacccgtg aggctgtcga
      421 gcgtgccgta ggtcttggcg acgaccaccg ggtggcgcaa cgccggcagc aggatggtcg
      481 tcgcgagcag aatgcgctcg gtgacggcgg ccgcggcggt taggatgctc gggccgtcga
      541 gccagccggt gcgcgaggcg aggtggtcgt tggcgccgag caggcggtag ccgagccgct
      601 cggcggcgag cgcgtacgcg gtgaagtcgc gcagccgcgg ccgctcgccc gccccgatga
      661 ccaccgggat gtgcgcgccg aactccatgc gtgattctcc tcaatccgtg gcggtgccgc
      721 tcgtctccat cgagccgcgc aaccccaggg actgctaggc acttgctccg aaacgccgtg
      781 ccggcgcgga tcctgtaggc cagagctgac cgacgattcc gcagcgcgac gagagtttca
      841 aacgggttcc caccacgctc tagcttgcca cgaggctctc ccccgtgtac aagaatcgaa
      901 gtgagccgcg agacatcgga gtagactcac cgcttcctca agcgcgaaga caaggcgctg
      961 cgagattctg accccgaact ggccctggtg gctgttccgt ggcattgctt cgtgataccg
     1021 caggtggtac tatgagcctg aggcgatgag tcgtcgcgac aaattgatcg cgcgcattct
     1081 agcgcgccca ccgcaagccg actttgatga tgtgcgcgcg gtgctggagg aatttggttg
     1141 ggtgctcgcg cgagagcggg gaagtcactg cacgttcgcc aagcctgggg aatacccgat
     1201 catcgttccc aaagtgggtg ggcggaaggt caagcgcgtt tacctggaca tgatctgcga
     1261 ccgcctcgga ttggatgact actcatgagt accacgcagc gcaagccact cgaaacctac
     1321 ctgtcactcg agtatccctt tgaggtacgt gttgacccgg atggcggcta cgtcgtggcc
     1381 tttcccgatc tccccggttg tatgacgcag gtcgagagtc tggacgaggt cggtcccatg
     1441 gccgaggaaa tccgcactct ctggatcgag actgcctacg atcagggcct ggagattcca
     1501 acgccttcgt gccgcgaagc ctacagcggg aagttcaacg tgcgcctgcc acgctcactg
     1561 catcgtttcc tggctgaatc cgccgagcgt gagggggtca gcctgaacca gtatgtgctg
     1621 atgctcctgt ctcagcggga tgctgaggcg cgcatcgcca gccggctcgc ggcgctggaa
     1681 gctcagttgg aggcgattca cgtcgggaat cgttcccgat cagccgcgta acggccttgc
     1741 ggccggtacg cacctcgtac cgcccggctg ccagttctct cgcctcggat tgaccgtgcc
     1801 cggcgccaaa cgcatactgg ccccaaccaa agcgctacgc aaccacagtc cgcttgtgaa
     1861 ccggagttgc caaccaaaag gagccgcgct catgttcgag ggtgagatcg tcggccgacc
     1921 gctggcggag gtcgatacgc cggccctggt catcgacgcc gacgcgctgg agcgcaatat
     1981 cgcgcgcatg gcggagttct tccagggcaa gcacgccaag ctgcgcccac acgccaagac
     2041 gcacaagtcg ccggtgatcg cccagatgca gctcgcggcg ggggccgtcg gcatcacctg
     2101 cgccaagctg ggcgaggccg aggtgctggc ggccggcggg gtgcgcgata tcctgatcgc
     2161 caaccagatc gtcggcgagc gcaagatcgc gcggctggtc ggtttggcgc ggcacgccaa
     2221 cgtggcggtg gccgtcgacg acttcgccaa cgccgccgcc atctcggagg cggccctggc
     2281 ggcaggttca accgtgcgcg ccctggtcga ggtcaacatc ggcatgaacc gctgcggcgt
     2341 cgagccgggc gcgccggcgg tcgagctggc gcagcgggtg gcctcgctgc cggggctgcg
     2401 cttcttcggg ctgcagggct acgaaggaca cctggtgacg gtgcccgagc acgccgagcg
     2461 cgagcagcgc gtgcgcgcgg cgatggcgcc gctgatcgag acgcgccgcg ccatcgaggc
     2521 ggccggcatc ccggtcacgg cggtcagcgg cggcggcacc ggcaccttcg acatcaccag
     2581 taccatcgac ggcttcgacg agatccaggc cggctcctac gtcttcatgg acgcctccta
     2641 cggcaaggtc gaggggccgg gggccgcctt cgagccggcg ctcttcctct ggtccaccat
     2701 catcagccgc ccggcgcccg agcgcgcggt ggccgacatc ggcctgaaaa cggtcaccag
     2761 cgaccacggg ctgccggtgc tggccggtat gcagggggcc gaggtcctca gcctctccga
     2821 ggagcacgcc aagctgcacg tcgagggcga ggcgcgcggg ctgcgcgccg gcgacaaggt
     2881 gcgcttccag ccgggccacg tctgcaccac ggtcaacctg cacgacgcct acgtcgtggc
     2941 ccgcggcggc atcgtcgagg ccgtctggcc ggtggcggcg cgcggccgct cgcgctagga
     3001 gcgacggcca cggggagccg ccggtcgatt cggccgtctt cgtaccacgg ttgtgccgtt
     3061 tgttgctgat gagagatcct tcggctgcag ccgaaggatc tcctcgaggc ctcggccaga
     3121 cagggtgtga cgggatcagt cctctgggtg gcaaacttgg agcagcgttg atcgagcgag
     3181 cgacgaaggt gaggcgacgc ggtgagcacg gccaaggagg cgacgcggct ggtgacggct
     3241 gaggagctgt gggcgctacc cgagcagccc ggcgtgcgct acgaactggc ggaaggggag
     3301 ctggtcgagg tgccgggtgc tggagcgttg cacaatctga tcgcggcgtt ggtctatcgg
     3361 ctgatctacg ccgccgcgcg gggtcagaac cttggcctgg tcttcaccga cggcgtgggc
     3421 tacatcctgc gccgcgcccc cgacgttctg cgcatcccgg atgtctcctt cgtcgcgcgc
     3481 gagcgcatcc ccgccggcgg catccccgag ggcttctggc cgggcgcgcc cgatctggcg
     3541 gtcgagatcg tctcccccaa cgatctggca caagagatca acgataaggt gcatcagtac
     3601 ctggaggtcg gcacgcgcat ggtctgggtc ctctggccga accgccgcac ggtgaccgtc
     3661 tacttgccgg aagggcagct ccgcgatctg gggccaaacg acgagctgga cggcggcgag
     3721 gtgctgcccg gcttcagcgt gcgcgtggca tcgctcttcg aggtcgcgca gtagcgactg
     3781 cccctgtcca ggggtacctg tcccaagggg caatccggcg ccttgaccgg gctcacccgc
     3841 gcgggtatgc ttaggtcaga attgacgaga gtttttcttc accaacacgg tcttcgtctg
     3901 accaatgatc gtgagacgaa ccgcggaggc ggcggcggtg gagacggtct tcctgatctg
     3961 cttcgtcttt ggcgcggcct tcgcgctcct ctccctcctg ctcggcttcg ccgggcacac
     4021 ctggcatgtc gagcacctgt cgcacgtcca cctgcaccac gacgtgcagg ttcatgccgg
     4081 cggccacgac ggacagctcc acgtggacca gggcgaggcc catctgccga tcttcaacgt
     4141 ctcggccctg ctcgccttcc tgacctggtt cggtggcgcc ggctacctgt tggcgcgcct
     4201 cagcggctgg ccgctgctgc tggtgctggc ggcggcggtg gtcgtcgggc tggccggcgc
     4261 ggcgctgatc ggcctgatcc tgggcaagat cctggccggt gagcgggtga tgaatccccg
     4321 ggactatcag ctcgaaggta ccctggcgcg ggtgacggtt agcatccctg ccggtggcgt
     4381 cggcgagatc atcttttcca aggcggggag ccgccgtggc gaggccgcgc gcagcgtcga
     4441 cggccgcgcc atcgcgcgag acacggaggt ggtggttatc gactacgctg ggggagtagc
     4501 gctggtggag agctgggagc agctcgtgag ccgccccgag cgcgtgccgc tcgccggcga
     4561 tcgttagaga ggactcgttc atgggcgtga tcatcgccat cgtcgtcgtc gccatcctga
     4621 ccatcatcgt gatcaccgcc ggcatcggca gcatctaccg gcgcgtcagc cccaaccgtg
     4681 ccctgatcgt cttcggctcg ggcggcaccg ccatcgtcac cggcggcggc aagctcgtct
     4741 ggccgctctt ccagagctac caggagctgt cgctggagct gatgtcgttc gacgtcgccc
     4801 cctcgcagga cctctacact gcccagggcg tggccgtctc ggtcgaggcg gtggcgcaga
     4861 tcaaggtgaa gtcggacccc gagagcatcc gcaccgccgc cgagcagttc ctgaccaaga
     4921 cgcagctcga gcgcgaggag ctgatccggc tggtgatgga ggggcacttg cgcggcatcg
     4981 tcggcctgct gacggtggag cagatcgtca aggagccgga gatggtcgcc ggccgggtac
     5041 gtgagaccgt ggccgacgac ttgagcaaga tgggactgga ggtcgtctcc ttcaccatca
     5101 agcgcgtgac cgacgagaag gactacatca ccaacatggg ccggccggac gtggcgcgca
     5161 tcaagcgcga ggccgatatt gcccaggccg aagccgagcg cgacaccgcc atcaagcgcg
     5221 ccatggcggc gcgcgagtcc gccatcgccc aggcacaggc cgaccaggag cgggttatcg
     5281 ccgagaccgc gtcgcagacc aagcaggccg aggccacgcg cgacctggaa ttgaagcggg
     5341 ccgagtacga cgccgccgtg cgccgcgagc gcgccatcgc cgagaaggcg caggacatcg
     5401 ccgccaacca ggcgcagcag caggtggtgg ccgagcaggt caaggtggag caggtcgaga
     5461 agcaggggct cattcaggtg caggagctgg aggtccagcg gcgcgagcgc gagctggagg
     5521 cgacggtcat caagcaggcg gcggccgagc agcgcaagat cgagctgctg gccgaggccg
     5581 agcgcgagcg gctgatccgc caggcgaccg gccagtccga ggctacccgg ctgcaaggga
     5641 ccgccgaggc cgagatcgtg cgcgccaagg ggcaggccga ggccgacgcc atgcacctgc
     5701 gcgccgaggc ctaccaggag tacagccagg cggccatgct cgacaagctg ctgggcagcc
     5761 tgccgcagtt ggcgcaggcc ttcgccaact cgctcgccag cgtcgataag gtgacgatcc
     5821 tctcgaccgg cgacggccgc aacggcggcg gtggcatgag cggctacacc ggcgagctgg
     5881 ccaagatggt ggcccaggtg ccggagctgt tcgagacgct gaccggcatg aagatgagcg
     5941 agacgatcgg ccgcctgcaa gggctgcagc cgaccaagcc gcccaagccg gccgcgccga
     6001 cggtgccggc caacgggcac gccgccccgg tcccgcccgc cgaggtcggc ccgggcgaga
     6061 aggaggagta acggccctca cccccgacgg ggc
//
LOCUS       DASUBI010000540         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_315244, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000540 DASUBI010000000
VERSION     DASUBI010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315244"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU72_10400"
     CDS             <1..197
                     /locus_tag="VFU72_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU5423940.1"
                     /translation="TRPGGTGYRRRIDSAMLDAVSWPAARKPLVYIAGPTSFVETAAG
                     GLLDLGYDSSRIFTEWFSPR"
     gene            349..3519
                     /locus_tag="VFU72_10405"
     CDS             349..3519
                     /locus_tag="VFU72_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5423941.1"
                     /translation="MSERASGVQVDPAMLQALEWRLVGPHRGGRVVAVAGHPSEYGTF
                     YFGGCAGGVWKTTDAGVYWQNISDGYFNVGAIGALALAPSDPNVIYAGTGETAIRGNV
                     SHGDGVYKSTDGGRSWRHMGLEDSHAIARVRVHPENPEHVYAAVFGHVWGPSAERGVY
                     RSTDGGEHWQKVLSRDDHSGAIDLSLDERNPRILYATLWDAQRYPHALRSGGPGSGIF
                     RSFDGGDSWEEITRNPGLPEGVLGKIGIAASPAQAGRVYAVVEAHDGAVFRSDDYGST
                     WQRLSEQGDLRRRAWYYQHIFADPQDPDTVWVLNLKCWKSTDGGKNFFAIPTPHGDNH
                     DLWIDPANSNRMIEGNDGGANVTLNGGLTWSSIFNQPTAQFYHVTTDDRVPYRLYGSQ
                     QDNTALSVPSASARGAITQAEWFEPGGGESGYLAVKPNDNDVVVGGAIGSGSGHGRML
                     RFNHRTMETRIVTVWPEVHGSGRGAKHHKYRFQWTYPIMYSQHNPDALYACSNHVHRS
                     TDEGFSWDVISPDLTRNDAEKFEASGGPITKDNSGAEVYGTIFALAESPLQAGLFWAG
                     SDDGLVHISKDGGQSWQNITPPANLLPEWALISIIEPSPHDAGTAYVAATRYKHDDFA
                     PYIFKTSDYGQSWTKITEGIPANDFTRTVREDPTRKGLLYAGTETAVYVSFDDGAHWQ
                     RFQSNLPVVPIHDLLVKESDLIAATHGRSFWILDDLSPLRQLQGNEAQPRTQLFQPRP
                     VDRFRTYGRMGDAAAPYLTYGRTGGMVISWRPVPKANGQVEPEFVDAGKNPPSGAIVY
                     YSLASQPQEVTLTFADAGGNEIRSFSSKGNGKSNGQGQGPQPGTQVGLNRFVWDLRYA
                     PAAEVPGHPGSSPDALNGPIAVPGQYQVILTVDGQSQTQSFEVRKDPRVSGSQQDLQA
                     QFDLLLKIRDAISQTNKTVLQLRDVRAQVQDWAKRVDDPAIDEAARRLVERLNAVELE
                     LIQPEAASPLQPPIGLTDKFGALPGMIQNADTKPAKQYYEVFDKLSGELDAQLEQLDQ
                     ILRREVADFNQSLAQHQVKPVMVPEG"
BASE COUNT          624 a         1322 c         1148 g          495 t
ORIGIN      
        1 agacacggcc cggcgggacc ggctaccgcc gacgcatcga ctccgccatg ctggacgcgg
       61 tgagttggcc cgccgcccgg aagccgcttg tctatatcgc cggtccgacc tccttcgtgg
      121 aaaccgctgc cggcggtctc ctcgacctcg gctacgactc ctcgcgtatc ttcaccgaat
      181 ggttcagccc tcgctagtag ccgcccacgg cgcgcgccga cttgacgagt ccctagtaca
      241 gtgcgagaaa ggggctcgcg cctgagtgac cgggcgcgcg agcccagcga cagtgtgcgg
      301 agggtggccg ctccggaagc gacgcaccgg cacgggaagg acgaggagat gagcgagcga
      361 gcgagcggcg tccaggtcga tccagccatg ctgcaggcgc tggagtggcg gctggtcggc
      421 ccgcatcgcg gcgggcgcgt ggtggccgtg gccggtcacc ccagcgagta cggcaccttc
      481 tacttcggcg gctgcgccgg aggcgtctgg aagaccaccg acgccggcgt ctactggcag
      541 aacatctccg acggctactt caacgtcggc gccatcggcg cgctggccct ggctccctcc
      601 gaccccaacg tgatctacgc cggcaccggt gagaccgcca tccgcggcaa cgtctcccac
      661 ggcgacgggg tctacaaatc caccgatggc ggccgcagct ggcggcacat ggggctggaa
      721 gacagccacg cgatcgcccg cgtgcgcgtg catccggaga accccgagca tgtctacgcc
      781 gctgtcttcg gccacgtctg gggcccgagc gccgagcgcg gcgtctaccg ctcgaccgac
      841 ggcggcgagc attggcagaa ggtgctctcc cgcgacgacc actcgggcgc gatcgatctc
      901 tcgctcgacg agcgcaaccc gcgcatcctc tacgccaccc tctgggacgc ccagcgctac
      961 ccgcacgcgc tgcgcagcgg cggtcccggc agcggcatct tccgctcgtt cgacggcggc
     1021 gactcctggg aagagatcac gcgcaacccg ggtctgcccg aaggcgtcct gggcaagatc
     1081 ggtatcgccg cctcgccggc ccaggccggg cgcgtctacg ccgtcgtcga ggcccacgac
     1141 ggcgccgtct tccgctccga cgactacggc agcacctggc agcgcctctc ggagcagggc
     1201 gacctgcgcc gccgcgcctg gtactaccag cacatcttcg ccgacccgca ggaccccgac
     1261 acggtctggg tgctcaatct gaagtgctgg aagtcgaccg acggaggcaa gaacttcttc
     1321 gccatcccga ccccgcacgg cgacaaccac gacctctgga tcgacccggc aaactccaac
     1381 cgtatgatcg aggggaacga cggcggggcc aacgtcacgc tcaacggtgg cctgacctgg
     1441 tcgagcatct tcaaccagcc gaccgcgcag ttctatcacg tcaccaccga cgaccgcgtg
     1501 ccctatcgcc tctacggctc gcagcaggac aacaccgccc tcagcgtccc cagcgcctcg
     1561 gcgcgtggcg ccatcactca ggctgagtgg ttcgagccgg gcggcggcga gagcggctat
     1621 ctggcggtca agccgaacga caacgacgtg gtggtcggcg gcgcgatcgg cagcggcagc
     1681 ggccacgggc gcatgctgcg cttcaaccac cgtaccatgg agacgcgcat cgtcacggtc
     1741 tggccggagg tccacggcag cggacgcggg gccaaacacc acaagtatcg tttccagtgg
     1801 acctacccga tcatgtattc gcagcacaac cccgacgcgc tctacgcctg ctccaatcat
     1861 gtccaccgct ccactgacga gggcttcagc tgggacgtca tcagccccga cctgacgcgc
     1921 aatgatgccg agaagttcga ggcctcaggc gggccgatca ccaaggacaa cagcggcgcc
     1981 gaggtctacg gcacgatctt cgccctcgcc gagtcgccgc tgcaggccgg gctcttctgg
     2041 gccggctccg acgacggcct cgtgcacatc tcgaaggacg gcggccagag ctggcagaac
     2101 atcacaccgc cggccaacct tctgccggag tgggcgctga tctcgatcat cgagccctcg
     2161 ccgcacgatg ccggcacggc ctacgtggcc gccacgcgct acaagcacga cgacttcgca
     2221 ccgtacatct tcaagaccag cgactacggc cagagctgga ccaagatcac tgaaggcatc
     2281 ccggccaacg acttcacccg cacggtgcgc gaggatccca cccgcaaggg tctcctctac
     2341 gccggcaccg agacggcggt ctacgtctcc ttcgacgacg gcgcccactg gcagcgcttc
     2401 cagtcgaatc tgccggtcgt cccgatccac gatctgctgg tcaaagagag cgacctgatc
     2461 gccgccaccc acggccgctc cttctggatc ctcgacgacc tctcgccgct gcgccagttg
     2521 cagggcaatg aggcgcagcc gcggacgcag ctcttccagc cgcgcccggt ggaccgcttc
     2581 cgcacctacg gccgcatggg cgacgccgcc gcgccctacc tcacgtacgg gcgcaccggc
     2641 ggcatggtga tcagctggcg ccccgtgccc aaagccaacg ggcaggtcga gccggagttc
     2701 gtcgacgccg gcaagaaccc acccagcggc gcgatcgtct actactcttt ggcgagccaa
     2761 ccgcaggagg tcacgctgac cttcgccgac gcgggcggca acgagattcg ctccttctcc
     2821 agcaagggca acggcaagag caacggccag ggacaaggcc cgcagccggg cacccaggtc
     2881 ggcctgaacc gcttcgtctg ggatctgcgc tacgccccgg cggctgaggt gcccggccac
     2941 cccggctcca gccccgacgc gctgaacggt ccgatcgcgg tgcccggcca gtatcaggtg
     3001 attttgacgg tcgatggcca gagccagacg cagtccttcg aggtgcgcaa ggacccgcgc
     3061 gtctcgggga gccagcagga tctgcaggcg cagttcgacc tgctgctcaa gatccgcgac
     3121 gcgatctccc agaccaacaa gacggtcctg cagctgcgcg acgtgcgcgc ccaggtgcag
     3181 gattgggcca agcgggtcga cgacccggcg atcgacgagg ccgcccggcg gctggtcgag
     3241 cggctgaacg cggtcgagct ggagctgatc cagccggagg cggccagccc gctgcagccc
     3301 ccgatcgggc tgaccgacaa gttcggcgcg ctgccgggga tgatccagaa cgccgacacc
     3361 aagccggcca agcagtacta cgaggtcttc gacaagctct cgggcgagct cgacgcccag
     3421 ctcgagcagc ttgaccagat cctgcgccgc gaggtggccg acttcaacca gtcgctggcc
     3481 cagcaccagg tcaagccggt gatggtgccg gaggggtagg aggacgggcg ctcacccccc
     3541 cgccacaggg ctgccctcat cccccaaccc cttctcccgt gcgcgggag
//
LOCUS       DASUBI010000541         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1145974, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000541 DASUBI010000000
VERSION     DASUBI010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145974"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFU72_10410"
     CDS             <1..878
                     /locus_tag="VFU72_10410"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU5423942.1"
                     /translation="GNRERMIDKALASAADVVLLDLEDSVTPGAKDAARRTVAATLER
                     LAADTAGSRPLVYVRVNSGELLRTDLDATVRSALDGLVLPKADAPEAVEEADRLLARL
                     EEIRGLAPGALRLLPIVERARGLLAAPQVAAASPRVVALLFGGEDFSADLGLPAPRER
                     EAAELLYPRWAVAVAAAAAGVPAIDTIWADVRDEAGLLAQAHIARRLGYTGKAVIHPG
                     QIEPVNEVFSPTPAEIDDARRVVAAAEAGERAGSGAVALDGRMLDRPIVERARRTLDL
                     ARRIGPSTGSPSTDA"
     gene            1023..1466
                     /gene="mscL"
                     /locus_tag="VFU72_10415"
     CDS             1023..1466
                     /gene="mscL"
                     /locus_tag="VFU72_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU5423943.1"
                     /translation="MKTLDEFKTFILRGNVVDLAIAVVIGAAFGAVIAALVKDIITPL
                     IAAIGGQPDFSALSFTINNSKFLYGDFINAVVSFLIIALVIFFLVIKPLNALFRRLKP
                     ERPVDVVTKTCPHCLSDIPLAATVCAFCTKDVARTDGMVAPAVGD"
     gene            complement(1594..2955)
                     /locus_tag="VFU72_10420"
     CDS             complement(1594..2955)
                     /locus_tag="VFU72_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423944.1"
                     /translation="MQAQPALPGSPSDAGADSRVLARGRLFIAARLPLLLVALVAVTF
                     SFAGQPALYHQYRARALSDPATVRNLTHLGLSVEAFALYLVAFGLIISVFCFAVAALI
                     AVRRADQPMALFVALLLVLLGATFSGPIGAQGQHTPALAWLNSILSGLSFESVFLLLY
                     LFPEGRFAPRWTRWLALASLLLIVPAATFPSSRLNPDHWPAGVYALLVAGLLASGVFA
                     QVYRYRRFSEYVARQQTKWVILGFSLALSIYLAVVLVSAVWSGFQPGTLVQLVGAGIS
                     AAGILLIPASFGVAILRYHLWNIDVLIGRTLVYGSLTAILSGLYAALVGLFQRLFLDR
                     TGSSSNLAIVLTTLVVAASFTPIKNGVQVLVDRRYKPAPAPASAASTTTPAAGQPAVA
                     PPVPEPAHRQDVQRLEELTSQLALLRDELATRAEAEHELRELIARLESRVSRAQDHQR
                     PNQ"
     gene            complement(3012..3467)
                     /locus_tag="VFU72_10425"
     CDS             complement(3012..3467)
                     /locus_tag="VFU72_10425"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5423945.1"
                     /translation="MLKDGKQRIRYFWEDVFNGGKIDEVDKLFTSDFVLHDLADNQLR
                     TRDDLKNLIQGVRYETPSAQAVIQEQFSAEDDHVVTRFVLRVALPSNDASTGAAAQQS
                     SDAPEERLLELNAISITRLAGEQLAETWLLWESLRGQQTLVFFWRWPPW"
BASE COUNT          576 a         1230 c         1285 g          515 t
ORIGIN      
        1 cgggcaaccg ggaacggatg atcgacaagg cgctggccag cgccgccgat gtcgtgctgc
       61 tcgacctgga ggactcggtc acgcccggcg cgaaggacgc cgcccgccgg acggtggcgg
      121 cgacgctgga gcggctggcg gcggacacgg ccgggtcgcg tccgctggtc tacgtgcggg
      181 tcaacagcgg cgagctgctg cggaccgacc tcgacgcgac cgtgcgctcc gcgctcgacg
      241 ggctggtgct gcccaaggcc gacgcgccgg aggcggtcga ggaggccgac cggctgctgg
      301 cccggctgga ggagatacgc gggctggccc ccggcgctct acgcctgctg ccgatcgtcg
      361 agcgcgcgcg cgggctgctg gccgcgcccc aggtggccgc cgcttcgccg cgcgtggtgg
      421 cgctgctgtt cggcggcgaa gacttcagcg ccgatctcgg cttgccggcg ccgcgcgagc
      481 gtgaggccgc cgagctgctc tacccgcgct gggcggtcgc ggtcgcggcg gccgcggcgg
      541 gcgtgccggc catcgacacc atctgggccg acgtgcggga tgaagcgggg ctgctggcgc
      601 aggcgcacat cgcgcgccgg ctcgggtata ccggcaaggc cgtcatccac cccggccaga
      661 ttgagcccgt gaacgaggtc ttcagcccga cacccgcgga gatcgacgac gcgcggcgcg
      721 tggtcgccgc ggcggaagcg ggcgagcgcg ccggcagcgg cgcggtggcg ctcgacggcc
      781 gcatgctcga ccgcccgatc gtcgagcgcg cgcggcgcac gctggatctg gcgcgcagaa
      841 tcgggccgag cacgggttct ccctccactg acgcttaatc acctctcggc tggttgcgtt
      901 ttgttcggaa ccatcctata gtggtcacga gtgtgatgaa tcgcgcgaaa ctaccggtcg
      961 gttccgggcg tggtgccgcg gcttggcgct agccgatggc gagtcgaagg gaagggtgcc
     1021 cgatgaagac actcgacgag ttcaaaacct tcatcctgcg cggcaacgtc gtcgacctgg
     1081 ctatcgcggt cgtgatcggc gccgccttcg gtgcggttat cgccgcgctg gtcaaggaca
     1141 tcatcacgcc gttgatcgcc gccatcggcg ggcagcccga tttctcggcc ctcagcttca
     1201 ccatcaacaa cagcaagttc ctctacggcg acttcatcaa tgctgtggtc agcttcctga
     1261 tcatcgccct ggtcatcttc ttcctggtga tcaagcccct caacgcgctc ttccggcgcc
     1321 tcaagccgga gcgcccggtc gacgtcgtca ccaagacctg cccgcactgc ctgagcgata
     1381 tcccgctcgc cgccaccgtc tgcgccttct gcaccaagga cgtggcgcgg accgacggca
     1441 tggtcgcacc ggccgtgggc gactgaggcg gcgcgccggc cgggcaccgt cggagaccgc
     1501 gactcttctc ccgccccgtc gggctcagcg gctcaaactg cgcccttgga ccacgcgcgg
     1561 ggcgggaccg tggcgcacgg gcgacggcat gggctactga ttggggcgct ggtgatcctg
     1621 agcgcgggag acgcgtgact ccagccgggc gatcaactca cgcaattcgt gctcggcctc
     1681 ggcgcgcgtg gccagttcgt cgcgcagcag tgccagctgg ctggtcagct cctccaggcg
     1741 ctggacgtcc tgccggtggg ccggctcagg cacgggcggc gcgacggctg gttgccctgc
     1801 cgcgggcgtg gtcgtgcttg ccgcggatgc cggggcgggg gccggcttgt agcgccggtc
     1861 gaccagcacc tggacgccat tcttgatcgg ggtaaacgag gcagccacca ccagcgtggt
     1921 caacacgatc gcgaggttcg aagagctgcc ggtgcgatcc aggaagagcc gctggaagag
     1981 cccgaccagc gccgcgtaga gaccgctgag aatggccgtc agcgagccgt agaccagcgt
     2041 gcggccgatg aggacgtcga tattccagag gtggtagcgc aggatcgcga cgccgaagga
     2101 ggccgggatc agcaggatgc cggcggcgct gatccccgcg ccgaccaact gcaccagcgt
     2161 gcctggctga aaccccgacc agaccgccga gaccagcacc accgccagat agatgctgag
     2221 cgccaggctg aagccgagga tcacccactt ggtctgctgg cgcgccacgt actccgagaa
     2281 ccggcggtag cggtagacct gggcgaagac cccgctcgcc agcaggccgg ccaccagcag
     2341 cgcgtagacg ccggccggcc agtggtccgg gttgagccgc gagctgggga aggtcgcggc
     2401 cgggacgatg aggagcagcg aggcgagcgc cagccagcgc gtccagcgcg gcgcgaagcg
     2461 gccctccggg aagaggtaga gcagcaggaa gacgctctcg aagctgaggc cgctcaagat
     2521 gctgttcagc caggccaggg ccggcgtgtg ctgcccctga gcgccgatcg ggccggagaa
     2581 ggtggcgccg agcaagacca gcaagagcgc cacgaagagc gccatcggct ggtccgcgcg
     2641 gcgcacggcg atgagcgccg ccaccgcgaa gcagaacacg ctgatgatca ggccgaaggc
     2701 caccagatag agggcgaagg cctcgaccga caggccgagg tgcgtcagat tccgcacggt
     2761 cgccgggtcg gacagcgcgc gggcgcgata ctgatggtag agcgccggct ggccggcgaa
     2821 cgagaaggtc acggcgacca gcgccaccag cagcaacggc aggcgagcgg cgatgaagag
     2881 ccgccctcgg gcgagaaccc gcgagtcggc tccggcgtcg ctcggcgatc cgggcagggc
     2941 cggttgtgcc tgcatcgtat cctgctccca cggccgccgc gacccgcgcc gggcctggcg
     3001 gccaggccgt gctaccaggg cggccagcgc cagaagaaga ccagcgtctg ctgaccgcgc
     3061 agcgactccc agagcagcca ggtctcggcg agctgctcac cggccagccg ggtaatgctg
     3121 atcgcgttca gctcgagtag gcgctcctcg ggggcatctg aagactgctg cgccgccgcg
     3181 cccgtggagg cgtcgttgga cggcagcgcg acccgcaaca cgaagcgggt gacgacgtga
     3241 tcatcctcgg cggagaactg ctcctggata accgcctgcg cgctgggcgt ctcgtagcgg
     3301 actccctgga tcagattctt cagatcgtcg cgcgtccgta gctgattgtc ggcgaggtcg
     3361 tgcagcacga aatcggacgt gaagagcttg tcgacctcgt cgatcttccc cccgttgaag
     3421 acgtcctccc agaagtagcg gatccgctgc ttcccatctt tcagcaccgt acaccctccc
     3481 ttctaccatt cgcgcagcac tcagtatagc atggtcgctc cctccgatag ctccctggga
     3541 acgccgagtc gtcgcacgcc gggcaaccgt tcctgccgat ttgggccgtg tgctgcctgg
     3601 aaatga
//
LOCUS       DASUBI010000542         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_454704, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000542 DASUBI010000000
VERSION     DASUBI010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454704"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="thrS"
                     /locus_tag="VFU72_10430"
     CDS             <1..948
                     /gene="thrS"
                     /locus_tag="VFU72_10430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5423946.1"
                     /translation="LKPMNCPGHIMIYKSQVRSYRELPVRLAELGTVYRYERSGTLHG
                     MLRVRGFTQDDAHIFCRPDQVQAEIVGVLDLALEFARVFGYEYKVFLATRPEKSVGSD
                     ENWERATEALAAALREKGLEFEIDPGAGAFYGPKIDIKFLDSLGREWTGPTIQADFNL
                     PERFDVTYIGEDGERHRAAMIHRTVLGSMERFVGGLIEHFAGAFPVWLAPIQAEIIPI
                     ADRHLDYAREVALQLKEAGIRSEIDESSDRMQAKIRRAQLQRVPYMLVVGDKEAESGA
                     VAVRLRSNENLGPMPLAEFIALASRQIETKSLALTAAAG"
     gene            complement(1084..2061)
                     /locus_tag="VFU72_10435"
     CDS             complement(1084..2061)
                     /locus_tag="VFU72_10435"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5423947.1"
                     /translation="MTMVEKPVQIIDGHTDFLLSLAKTGRSFFEESQTGHVDLPRARK
                     GGIGAMLCAVYVPNADIPERALIQTLRGTDHLKRLVAESHGEMELVRNYAQLVSCLER
                     GVFGAILHYEGAEAIDPEFAVLRLAYELGLRSLGFTWSRTNIFAEGVGPENKGHGLTA
                     LGKLLVENCNRMGILIDVSHLNVPGFWDVLETSTKPIVASHSNALSVCDHERNLTDRQ
                     LKELAAKGGLAGINYALGFLVKDAKKPADVPLSALVDHIDHIVEVAGIDHVALGSDFD
                     GAGVPEALRDAAHDQAIIEELARRGYDDASIRKIARDNWLRVLQDVWVE"
     gene            complement(2058..2900)
                     /locus_tag="VFU72_10440"
     CDS             complement(2058..2900)
                     /locus_tag="VFU72_10440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5423948.1"
                     /translation="MRRVGSYDVEDPHKIQGRDEVAVSDRSLAALRLRAALDALAGAR
                     GRLLVLGCGAGRYVRALERERPDLTLHGGDLSLTALHEAAARDERGQYLALDASRLPY
                     RDDVFGAVVFFDLLEHVPAYQAMLAEIARVLAPGGVLHFFVPLEAEPGTLYTLLAGSE
                     RLPIHRWKRDHVGHVNRFDAGSAIRAVWQAGLEVTDASFGFHLAGQVHDIVDYWNRER
                     QLGGPGLLPRRAVGAVTRATFLATWRLSYFEDRLYAGRRFASGLHLTAIKPGDPGKVK
                     EDER"
     gene            complement(2897..3727)
                     /locus_tag="VFU72_10445"
     CDS             complement(2897..3727)
                     /locus_tag="VFU72_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU5423949.1"
                     /translation="MGRTVTGSRELAEALAPIRGWVIDMDGVLYRGQTALPHVSRFLS
                     TLDARDLPYILATNNSMQTPRQYVEKLARMGVTVRPERILTSALATAVWLKPRYPRGT
                     PAYVVGMPALEEAIYGDGYFVPATTGARVVVSGLDLTLTYEKLKLADLALRGGAAYVA
                     TNADKTLPTEEGLLPGSGAIVAALTAASDVEPTIIGKPATGMLEASLTELGLPASRAA
                     MLGDRLDTDILGGNRAGMLTVMVLTGVNTLADLAVSEIVPDVVVPDLAPLVAFYGETQ
                     "
     gene            3856..>4428
                     /locus_tag="VFU72_10450"
     CDS             3856..>4428
                     /locus_tag="VFU72_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5423950.1"
                     /translation="MSQVQKIDPALAQRVLDQIKQDEAISFLQALVRVPSVNPPGDVR
                     DAIKVCSDKLKSEGFDLSFITGVDTIPNLVARLGPSGGRTLLFNAHVDVVPTGEESAW
                     SHPPFAADIADGRVYGRGAGDDKASVTAQVMAGIALARSGVPLKGQLIVNEVGNEEVG
                     GVNGAQLVAQSPEVHPDWVIVGEQTLNRIAI"
BASE COUNT          700 a         1590 c         1487 g          651 t
ORIGIN      
        1 ctgaaaccga tgaactgccc cggccacatc atgatctaca agtcgcaggt gcgctcctac
       61 cgcgagttgc cggtgcggct ggccgagctg ggcacggtct accgctacga gcgcagcggc
      121 acgctgcacg gcatgctgcg cgtgcgcggc ttcacccagg acgacgccca catcttctgc
      181 cgccccgatc aggtgcaggc ggagatcgtc ggcgtgctcg atctggccct ggagttcgcc
      241 cgcgtcttcg gctatgagta caaggtcttc ctggccacgc ggccggagaa atcggtgggc
      301 agcgacgaga actgggagcg ggctaccgag gccctcgcgg cggccctacg cgagaagggg
      361 ctcgagttcg agatcgaccc cggcgccggc gccttctacg gcccgaagat cgacatcaag
      421 ttcctcgact cgctcgggcg cgagtggacc ggccccacca tccaggccga cttcaacctg
      481 ccggagcgct tcgacgtgac ctacatcggc gaggacggcg agcgccaccg cgccgccatg
      541 atccaccgca cggtgctcgg ctcgatggag cgcttcgtcg gcgggctgat cgagcacttc
      601 gccggcgcct tcccggtctg gctggcgccg atccaggccg agatcatccc catcgccgac
      661 cgccacctcg actacgcccg ggaggtcgcg ctccagctca aggaggccgg catccgctcg
      721 gagatcgacg agagcagcga ccgcatgcag gccaagatcc gccgcgccca actgcagcgc
      781 gtgccctaca tgctggtcgt cggcgacaag gaggccgaga gcggcgccgt cgccgtgcgc
      841 ctgcgcagca acgagaacct gggccccatg cccctggccg agttcatcgc cctggccagc
      901 cgccagatcg agaccaagtc gctggcgctg acagcggccg cggggtagcc ctcatccccc
      961 ccgccccgca cggagtcttc tcccaatact gggagaaggg ggagaagtag ggggaacacg
     1021 gccctgttcc cccgcgtgct cccctctccc agcattggga gaggggttgg gggtgagggc
     1081 gcgctactcc acccagacgt cctgcagcac gcgtagccag ttgtcgcggg cgatcttgcg
     1141 gatcgaggcg tcgtcgtagc cgcggcgcgc cagctcctcg atgatcgcct ggtcgtgcgc
     1201 cgcgtcccgc agcgcctccg gcacccccgc gccatcaaag tcggagccga gcgcgacgtg
     1261 gtcgatcccc gccacctcga cgatgtggtc gatgtggtcg accagcgcgc tgagcggcac
     1321 gtccgccggc ttcttagcgt ccttgaccag gaagccgagc gcgtagttga tccccgccag
     1381 gccgcccttg gccgccaact ccttcagttg ccgatcagtc aggttgcgct cgtggtcgca
     1441 gacgctgagc gcgttcgagt ggctggcgac gattggcttg gtcgaggtct ccagcacgtc
     1501 ccagaagccg ggcacgttca ggtgcgagac atcgatgaga atgcccatgc ggttgcagtt
     1561 ctccaccagc agcttgccca gcgcggtcag cccgtgcccc ttgttctccg gcccgacgcc
     1621 ctcggcgaag atgttggtac ggctccaggt gaagccgagc gagcgcaggc ccagctcgta
     1681 ggccagccgc aatacggcga actccgggtc gatcgcctcg gcgccctcgt agtgcaggat
     1741 ggcgccgaag acgccccgct ccaggcagct caccagttgc gcgtagttcc gcaccagctc
     1801 catctcgccg tgcgactcgg ccaccagtcg cttgagatgg tcggtgcccc gcagggtctg
     1861 aatcagcgcg cgctcgggga tgtccgcgtt gggcacgtag acggcgcaga gcatggcgcc
     1921 gatgccgccc ttgcgtgcgc gcggtaggtc gacgtgcccg gtctgcgact cctcgaagaa
     1981 gctccggccg gtcttggcca gcgagagcag aaagtcggtg tgcccgtcga tgatctggac
     2041 cggcttctcc accatcgtca tcgctcgtcc tccttcacct tccccgggtc tcccggcttg
     2101 atcgccgtca ggtgcaagcc ggaggcgaag cgccgcccgg cgtacagcct atcctcgaag
     2161 tacgacaggc gccaggtggc tagaaaggtg gcccgtgtca cggcgccgac cgcccggcgc
     2221 ggcagcagcc ccggcccgcc caactggcgc tcgcggttcc agtagtcaac gatgtcgtgc
     2281 acctgcccgg ccaggtggaa gccgaaggag gcatcagtca cctccagccc ggcctgccag
     2341 acggcgcgga tggccgaacc agcgtcgaag cgattgacgt ggccgacgtg gtcgcgcttc
     2401 cagcggtgaa tcggcagccg ctcggagccg gccagcagcg tgtagagcgt gcccggctcg
     2461 gcctccagcg gcacgaagaa gtgcagcacg ccgcccggcg ccagcacccg cgcgatctcg
     2521 gccagcatcg cctgataggc gggcacgtgc tccagcaggt cgaagaagac gacggcgccg
     2581 aagacgtcgt cccggtaagg aagccggctt gcatccagcg ccaggtactg cccgcgctcg
     2641 tcccgcgccg ccgcctcgtg cagtgcggtg aggctgaggt cgcctccgtg aagggtcagg
     2701 tcgggccgct cgcgctccaa cgcgcgcacg taacgccccg cgccgcagcc aagcaccagg
     2761 agccgcccac gcgcgcccgc cagcgcgtcc agcgcggcgc gcagcctgag cgccgccagg
     2821 ctccggtcgg agacagcgac ctcgtcgcgc ccctggatct tgtgcgggtc ttccacgtcg
     2881 taggagccga cgcgcctcat tgggtttctc catagaacgc caccagcggc gcaaggtctg
     2941 gcacgaccac gtccggcacg atctccgaga ccgccagatc ggccagagtg ttcactcccg
     3001 tcagcaccat caccgtcagc atgccggcgc ggttgccgcc cagaatgtcg gtgtcgagcc
     3061 ggtcgccgag catggccgcc cggctcgccg gcaggcccag ctccgtcagg ctggcctcca
     3121 gcatgccggt ggcgggcttg ccgatgattg tcggctcgac atcggaggca gcggtcagcg
     3181 ccgccacgat cgcgccggag cccggcagca gcccctcctc ggtcggcagc gtcttgtcag
     3241 cgttggtcgc gacgtaggcc gcgccgccgc gcagcgccag atcggcgagc ttgagcttct
     3301 cgtaggtcag cgtcaagtcg agcccgctga ccaccacccg tgccccggtt gtagccggca
     3361 cgaagtagcc gtcgccgtag atcgcctcct ccaaagccgg catgccgacg acgtaggcgg
     3421 gcgtgccgcg cgggtagcgc ggcttgagcc agacggcggt ggccagcgcc gaggtcagga
     3481 tgcgctcggg ccgcaccgtc acgcccatgc gcgccagctt ctccacgtac tgccggggcg
     3541 tctgcatcga gttgttggtg gccaggatgt aggggagatc gcgcgcgtcg agcgtgctga
     3601 ggaaccgcga gacgtgcggc agcgcggtct ggccgcggta gagcacacca tccatgtcga
     3661 tgacccagcc gcggatcggc gccagcgctt cggccagctc ccgcgacccc gtgacggtcc
     3721 tccccacgac gcccccttca tgcgcatcca gtcgaccagc ggcgaggata ccacagcctc
     3781 ccgccgcgcc agcccgcgga aaagacggtg ctatactgcc gggaagtcgt ggcggtggga
     3841 tatgaggagg atgtcgtgag ccaggtccag aagatcgatc cggcgttggc ccagcgcgtg
     3901 ctcgaccaga tcaagcagga cgaggccatc agcttcctgc aggcgctggt gcgcgtgccg
     3961 tcggtcaatc cgcccgggga cgtgcgcgac gcgatcaagg tctgcagcga caagctcaag
     4021 agcgagggct tcgatctcag cttcatcacc ggcgtcgaca cgatccccaa cctggtggcc
     4081 cggcttggcc cgagcggcgg cagaaccctc ctcttcaacg cccacgtcga cgtcgtgccg
     4141 accggcgagg agtcggcctg gagccatccg cccttcgccg ccgacatcgc cgacgggcgc
     4201 gtctacgggc gcggcgccgg cgacgacaag gccagcgtca cggcgcaggt gatggccggc
     4261 atcgcgctgg cccgctccgg cgtgccgctg aaggggcagc tcatcgtcaa cgaggtcggc
     4321 aatgaggagg tcggcggcgt caacggcgcg cagctcgtcg cccagtcgcc cgaggtccac
     4381 cccgactggg tcatcgtcgg cgagcagacg ctgaaccgca tcgccatc
//
LOCUS       DASUBI010000543         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1219140, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000543 DASUBI010000000
VERSION     DASUBI010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219140"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFU72_10455"
     CDS             <1..194
                     /locus_tag="VFU72_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423951.1"
                     /translation="MGTAPLGSFVVGWVAEQYGVADAIMLMTGLCALGVVLGLLYLAR
                     HRTPAGSVKTREREAALHD"
     gene            complement(446..1777)
                     /locus_tag="VFU72_10460"
     CDS             complement(446..1777)
                     /locus_tag="VFU72_10460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423952.1"
                     /translation="MAGSTLLEAPRGKRPSPRAALRQTFRALRNRNYRLFWSGQVISS
                     VGTWMQRIALAWLVLKLTNSPLDLGLVTTFQTLPVLLFALFGGVIADRVAKRRLLLIT
                     QSVMLLQASVLALMTAAGMDNLYAIYVLAAILGTASALDNPARQAFVKELVGPDDVAN
                     AVALNSIVFNTARLIGPALGGVTIAAVGVTGCFTLNALSFLAVLGGLIMMRPADFYEL
                     ARPPRGKVLRQIAEGVQYAVRTPDIAVVLLLMLVIGTFGYNFTVILPLIATYVLHAGP
                     VGFGTLTSSMAIGSLVAALGIAYTGRVSQRTLLAGASGFSGLLLLLAVSDRWPTTIAI
                     LIALGLFSIIFTATANSRLQLVTPPELRGRVMSLYTLLFMGSTPIGSLVIGAMADHQG
                     VRLAVGELAILCWLGVLAGLLYLRRQRAPRSASTATPYEEPAPAITRSTSV"
     gene            complement(1897..2820)
                     /locus_tag="VFU72_10465"
     CDS             complement(1897..2820)
                     /locus_tag="VFU72_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5423953.1"
                     /translation="MLLDGRQSINREATMTTHLRLASFPHVSYLPQFIAQALGYFADE
                     GLDVSLAPHTGSWGALLAAPGERAVDLIIGNIWFALRQPGRLLPAAHGMQHSRFILYR
                     RPEHHEEFNWHQLDGAVVLIASEVPTPWIAFREALRLQGLSLDRVRAVTGYPAGDAAA
                     DLARGQADFALLGIEDGMRHGLAEAASLATTIGPVPWSVYLANAGDLAARAPVYRAFQ
                     RAIGHALAAIQERASAELAALAAPWFPGLDQTTAARIIGRYRALDAWPATPDIEPEHV
                     SRWHDAMLRWGLLHDPVDWQPLLRFSNGADF"
     gene            complement(3002..>4482)
                     /locus_tag="VFU72_10470"
     CDS             complement(3002..>4482)
                     /locus_tag="VFU72_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5423954.1"
                     /translation="MAAAALDHRGIVPIYDWGESDDTYYLVMAYVPGEDLKAALARRG
                     PLPEAEALGIARQVAAALAAAHARGIVHRDIKPHNILLQPDGQARVTDFGIARALDQA
                     PEGGLVLGTALYLAPEQARGEAVDARADLYSLGVVLFELLTGRTPFAGESLPALARQH
                     LERTPDAPSALRAGLSAATDEVVLRALAKEPEQRYQSAAELEAGIDAALAGSMAAGRR
                     VAAHVPLLRLTPGELAVGRPLARYPAHARPAVRPLPRPAGDWSLTLGLVVFLLAGLAG
                     VVMVHAARSSGAAATGQESNGGGVAMAATPISTESPNSAAPRTPAPLPTATPVPTPEV
                     ALARPTPTPTPTPAPASPTPTPRPAPTPTPTPIPTPTPEPKSGISGWFERVTDSPTTA
                     VGRFYLLVNQHKYDQAAALWDDRMRAAYPPREYIDGRFADTSWIWVQRDSLQANNGRR
                     AVVAVELLERKQSGESIRWTGTWTLVKTGAGWLLDQPDLRQG"
BASE COUNT          712 a         1506 c         1642 g          622 t
ORIGIN      
        1 acatgggcac cgcgccgctg ggcagcttcg tggtcggctg ggtggccgag caatacggcg
       61 tagccgacgc catcatgctg atgaccgggc tctgcgccct gggcgtcgtc ctcggtctgc
      121 tctacctggc acggcaccgc acgcccgccg gctcggtcaa aacccgcgag cgcgaggcgg
      181 cgctgcacga ctgagacggt gggctcttgt gacgtgcgcc gtggcccgtg gagattgggt
      241 accgcgcggc gcgggaatcc agccaccgcg ctgaacacga agcatagtgc aggggctgag
      301 cttccatgcc ccacgcggcg caaggcagtt gaacacgtgc gattgacaca gtggcatcaa
      361 ccgtgacgcg tcgacgataa gtcgctcgtg gagagccccg caccgcatcc aaggagttcg
      421 agcagttgga cgacgaactg acgggtcaga ccgacgtcga gcgcgtgata gccggtgcgg
      481 gctcctcgta tggcgtcgcg gtggacgccg agcgcggggc acgctgccgg cgcaggtaga
      541 gcagaccggc cagcacgccc agccagcaga ggatcgccag ctcgccgacg gccaggcgca
      601 cgccctgatg gtcggccatc gcgccgatca ccaggctgcc gatcggcgtc gagcccatga
      661 agaggagcgt atagaggctc atcacccggc cgcgcagctc gggcggcgtc accaactgca
      721 ggcggctgtt ggcggtcgcg gtgaagatga tgctgaagag gccgagcgcg atcaggatcg
      781 cgatggtggt gggccagcga tcggagacgg cgagaaggag cagcagcccg ctgaagccgc
      841 tggcgcccgc cagcagcgtg cgctgcgaga cccgcccggt gtaggcgatg ccgagggcgg
      901 ccaccagcga accgatcgcc atcgaggagg tcagcgtgcc gaagccgacc ggaccggcgt
      961 gcagcacgta ggtggcgatc agcggcagga tgacggtgaa gttgtagccg aaggtgccga
     1021 tcaccagcat caacaggagc accacggcga tgtccggcgt gcgcaccgcg tattgcaccc
     1081 cctcggcgat ctggcgcagc accttgccgc gcggcgggcg ggccagctcg tagaagtcgg
     1141 ccgggcgcat catgatcagc ccgccgagca cggccaggaa actgagcgcg ttgagcgtga
     1201 agcagccggt cacgccgacc gcggcgatgg tcacgccgcc gagcgccggg ccgatcaagc
     1261 gcgcggtgtt gaagacgatc gagttgagcg ccaccgcgtt ggcgacgtcg tccgggccga
     1321 ccagctcctt gacgaaggcc tggcgcgccg ggttgtcgag ggcgctggcg gtgccgagga
     1381 tcgccgccag cacgtagatg gcgtagaggt tgtccatccc ggcggcggtc atcagcgcca
     1441 gcacgctcgc ctgcaacagc atgacggatt gggtaatcag cagcagccgg cgcttggcga
     1501 cgcggtcggc gatcacgccg ccgaagagcg cgaacaggag caccggcagg gtctggaagg
     1561 tcgtcaccag gccaagatcc agcggcgagt tggtcagctt gagcaccagc caggccagcg
     1621 cgatgcgctg catccaggtg ccgaccgagg agatgacctg ccccgaccag aagaggcggt
     1681 agttgcggtt gcgcagggcg cggaaggtct gccgcagcgc ggcgcggggc gatgggcgct
     1741 tgccgcgtgg cgcttccagt aacgtacttc cggccatggc gcgacctcgc tctccgaaac
     1801 gccgcaggat ccgctacctg ccacgctatc gtactgcaaa tatattcaat gtcaatgtaa
     1861 cgggtggcag gatcgcggcc gcgcaggcaa ttggtactag aagtcggcgc cgttgctgaa
     1921 gcggagcagc ggttgccagt cgaccggatc atgcaggaga ccccagcgca gcatggcgtc
     1981 gtgccagcga gacacgtgct cgggctcgat gtctggggtc gcgggccagg cgtcgagcgc
     2041 gcggtagcgg ccgatgatgc gcgcggcggt ggtttgatcc aggccgggga accagggcgc
     2101 ggccagggcg gcaagctccg cgctcgcgcg ctcctggatg gccgccagcg cgtgcccaat
     2161 cgcgcgctga aaggcgcggt agaccggcgc gcgggcggcg aggtcgcccg cgttcgccag
     2221 atagaccgac caggggaccg ggccgatggt tgtggccagt gatgcggcct cggccagccc
     2281 atggcgcatc ccgtcttcga tcccgagcag cgcgaagtcg gcctggccgc gcgccagatc
     2341 ggccgcggcg tcgcctgccg ggtagccggt caccgcgcgt acacggtcga gcgagagtcc
     2401 ctgcagccgg agcgcctcac gaaaggcgat ccagggagtg ggcacctcgc tggcgatcag
     2461 gacgacggcg ccgtcgagct ggtgccaatt gaattcctcg tggtgctctg gccggcgata
     2521 gaggatgaag cgcgagtgct gcatgccgtg cgccgccggc agcaggcggc caggctggcg
     2581 cagcgcgaac cagatgttgc cgataatcag gtcgaccgcg cgctcgccgg gcgcggccag
     2641 caacgccccc cagctaccgg tgtgcggcgc gaggctgacg tcgagcccct catcggcgaa
     2701 gtagccgagc gcctgggcga tgaactgcgg cagatacgag acgtggggga agctagccag
     2761 gcgcaggtgg gtggtcatgg tcgcctctcg gttgatcgat tgtcggccgt cgagcagcac
     2821 gcgtgccgca cccgcacgga tccctcgact gcgctctgaa tgacagggtt aattcggggc
     2881 tgacgaagag gatgtgcctc gtccggtagg tcacaccctc gatgctgtca tcctgagcgc
     2941 agtcgaagga tccgtcctca caccataccc agctctcatc tgcagatact ccttccagcc
     3001 ctcacccctg ccgtaggtcc ggctggtcga gcagccagcc ggcgccggtc ttgaccagcg
     3061 tccaggtgcc ggtccagcga atactctccc cgctctgctt gcgctccagc agctcgaccg
     3121 cgaccaccgc tcggcggccg ttgttcgctt gcaggctgtc gcgctgtacc cagatccaac
     3181 tggtgtcggc gaagcgcccg tcgatgtact ccctcggcgg gtacgcggcg cgcatgcggt
     3241 cgtcccagag cgcggcggcc tggtcgtatt tgtgctggtt gaccagcaga tagaagcgtc
     3301 cgactgccgt ggtagggctg tcggtcacgc gctcgaacca gccggagatg cccgacttcg
     3361 gctcaggcgt gggggtgggg atgggagtcg gtgtgggcgt gggagccggc cgcggcgtcg
     3421 gtgtcggcga cgctggcgcg ggcgtcggcg tcggcgtcgg ggttggccgc gccagcgcga
     3481 cctccggggt tggaaccggc gtggccgtag gaagcggcgc cggagtccgc ggagcagccg
     3541 agttcggcga ttcggtcgag atcggcgtcg cggccatcgc gacgccgccg ccgtttgatt
     3601 cctggccggt cgcggcggcg ccgctggatc gcgccgcgtg caccatcacc accccggcca
     3661 gcccggccag caagaagacc accagcccca gcgtcaggct ccagtcgcca gccggtcgcg
     3721 gcagcggacg caccgccggc cgcgcgtgag cggggtagcg cgccagcggg cgcccgaccg
     3781 ccaactcgcc cggcgtcagt cgcagcagcg ggacatgggc cgccacgcga cgcccggccg
     3841 ccatcgagcc ggccagggcg gcgtcgatgc cggcctccag ctccgccgcg ctctggtagc
     3901 gctgctccgg ctccttggca agcgcacgca gcaccacctc gtcggtcgcc gccgaaagcc
     3961 cggctcgcag cgcgctgggc gcgtcaggag tgcgctccag gtgctgccgc gccagcgcgg
     4021 gaagcgactc gcccgcgaag ggcgtgcgtc cggtcaacag ctcgaacaga accacgccca
     4081 ggctgtagag atcggcacgg gcgtcgaccg cctcgccgcg cgcctgctcg ggcgccaggt
     4141 agagcgcggt gccaagcacc agcccgccct ccggcgcctg atcgagcgcg cgggcgatgc
     4201 cgaagtcggt gacgcgcgcc tgaccgtcgg gctgcagcag aatgttgtgc ggtttgatat
     4261 cgcggtgcac gatgccgcgg gcgtgcgcgg cggccagcgc ggcggccacc tgccgggcga
     4321 tgcccagcgc ctccgcctcc ggcagcggcc cgcgccgtgc cagcgccgcc ttgagatcct
     4381 cgcccgggac gtaggccatc acgaggtagt aggtgtcgtc gctctcgccc cagtcgtaga
     4441 tcggcacgat gccgcgatgg tcgagcgcgg ccgccgccat cg
//
LOCUS       DASUBI010000544         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_844941, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000544 DASUBI010000000
VERSION     DASUBI010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844941"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1576
                     /locus_tag="VFU72_10475"
     CDS             248..1576
                     /locus_tag="VFU72_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5423955.1"
                     /translation="MMIPVPASISVSVTVVVLLIALSRRFRARTLATVALCAACATLG
                     FQRLRIVDSLSLSDYCFLLATLLLIPTLMTQGSPRLRAHRLLLGAIVLMTAGGLVGTL
                     AASSVAASLSQLSHFALLTAWFVALIAIWSPNLREVRALLWCWIGSVTLTSLYAIFGG
                     GVDRDLNGRAYALTTHPNELAMICVLAAGPALAFVIAGRGWQRALALACSAIIPVGIL
                     FSGSRAGFFGYLAVLLVSVVIPWQRSTRAAAWAALSLAAAILLGSLVSSPTPNAVQRV
                     FAVQNPQVQHSLSYSDNERRELLETTLDRIEQHPFTGDGFEYFLSTHDIYLQTWSSAG
                     VIGLLGLALIILQTLRPTWLGLRGQDEDFLRGPAASLALGLSAGYAGYLVAALFQNAV
                     ADRYIWLAPALVVCLVPQFSVISLVEGRLGEALSSPLRAALALFSLPRQS"
     gene            1672..2058
                     /locus_tag="VFU72_10480"
     CDS             1672..2058
                     /locus_tag="VFU72_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5423956.1"
                     /translation="MPALTPQETELQFGAYFNAADLDSLLTLYESDAKLVAQPGQVVA
                     GIDQIRQALQGLLALHGRIQLTPQLTIEAGDIALVLSRWELKGTGPDGQPLELQGQTS
                     DVLHRHADGHWLFAIDNPWGAAATGG"
     gene            2298..>3365
                     /locus_tag="VFU72_10485"
     CDS             2298..>3365
                     /locus_tag="VFU72_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5423957.1"
                     /translation="MASREELRQRIVDAIEANRERIVAVGEEIRLNPEIGYEEVKASR
                     LLAANIRDHGYEVEKPAGGLETAFVAARHGRAEGPVVAVLAEYDALPGIGHGCGHNLI
                     AASGLAAAIGLNAVMDEIDGIFEVIGTPAEESGGGKIQLIDAGVFADVDASLMVHHGG
                     NNTDAASDYPNGRSLAVAHVDYEFFGKPAHAASDPWNGNNAVNAVVALFNGIDALRQH
                     IHMESRIHGIITHGGDAPNVVPNHAAAKFFIRAETMAYMQELVDKVARIAEGAALMTG
                     CELKITEGEVCYDMRPSYVVGGRYKEHMREMGLDINPRPAGRKASTDFGNVSYLMPSV
                     TGRFAISHEPIPGHSQQVVDAS"
BASE COUNT          504 a         1200 c         1117 g          544 t
ORIGIN      
        1 cgttgtgcgc gcgccgtgtg cgcctgccat aatgggccgt tgtccgtcgc gggggtgcgc
       61 aacggagtac catggctagg cgtgcggcgc tggtcattcg gagaggcgac gttcgtacgc
      121 tggcacgaga gttgccgtgg cgcaacctcg gcttcgcaag cggcaaccgg cacacccgca
      181 cgctgaaggg gaaaagcttg tctagagcac gatgtcccgg cggcacgccg aatctggagg
      241 tgccaggatg atgatcccgg tgccggcgag catctcggta tcggtcacgg tcgtcgtcct
      301 gctcattgcg ctcagtcgtc gcttccgagc ccgcacgctc gccaccgtcg cgctctgcgc
      361 cgcctgcgcc accctgggct ttcagcgcct gcgcatcgtc gacagcctca gcctgagcga
      421 ctactgtttc ctcctggcga cgctcctcct catccccacg cttatgaccc agggcagccc
      481 gcgcctgcgc gcgcaccgcc tcctgctcgg cgccatcgtc ttgatgaccg ccggcggcct
      541 ggtcggcacg ctggccgcct ccagcgtcgc ggcgagcctg tcgcagctca gccacttcgc
      601 gctgctgact gcctggttcg tggcgctgat cgcgatctgg tcgcccaacc tgcgcgaggt
      661 gcgcgccctg ctctggtgct ggatcggctc ggtcaccctg accagcctct acgccatctt
      721 cggcggcggg gtcgaccgcg atctcaacgg ccgggcctac gcgctgacca cgcaccccaa
      781 cgagctggcc atgatctgcg tgctggcggc cggaccggcg ctggccttcg tcatcgccgg
      841 ccgcggctgg cagcgcgcgc tggccctggc ctgctccgcg atcatcccgg tcggcatcct
      901 cttcagcggc tcacgggccg gcttctttgg ctatctggcc gtgctcctgg tgtccgtcgt
      961 gattccgtgg cagcgctcga cgcgcgccgc cgcctgggcg gcgctctcgc tggcggccgc
     1021 catcctgctt ggcagcctgg tcagctcgcc gacgccgaac gcggtgcagc gcgtcttcgc
     1081 cgtccagaat ccgcaggtgc agcacagcct gagctacagc gacaacgagc gccgcgagtt
     1141 gctggagacg acgctcgacc gtatcgagca acaccccttc accggcgacg gcttcgagta
     1201 cttcctctcg acccacgata tctacctgca gacctggtct tcggccggtg tcatcgggct
     1261 gctcgggctg gcgttgatca tcctccagac gttacgccca acctggctcg ggctgcgcgg
     1321 gcaggacgag gacttcctgc gggggccggc ggcctccctc gcgctggggc tcagcgcggg
     1381 ctacgccggc tacctggtgg ccgcgctctt ccaaaacgcc gtcgccgacc gctacatctg
     1441 gctggcgccc gcgctcgtcg tctgcctggt gccgcagttc tcggttatct cgctggtcga
     1501 ggggcgactc ggggaagcgc tgagctcgcc gctgcgggcc gcgctggcgc tcttcagcct
     1561 gccgcgccag agctagccgg ccttcctcgg ggtgggattg gcgcttatga tggtggcggg
     1621 gagagccgtc tctatcaggc gcgcccgcgc cgccagccga gaggagtgcc gatgcccgcc
     1681 ttgacgccgc aggagaccga gctccagttc ggcgcctact tcaacgccgc cgacctcgac
     1741 tcgctgctga cgctgtatga gtccgacgcc aagctcgtcg cgcagccggg acaggtggta
     1801 gccgggatcg atcagatccg gcaggccctg caggggctgc tggccctgca cgggcgcatt
     1861 caactcacgc cgcagctcac catcgaagcc ggcgatatcg ctctggtgct ctcacgttgg
     1921 gagctcaagg gcaccgggcc ggacggccag ccgctcgagc tgcagggaca gacgagcgac
     1981 gtcctgcacc gccacgccga cggtcattgg ctcttcgcca tcgacaaccc ctggggcgcc
     2041 gccgcgaccg gcggttgatc cgccaacgac atgatccgga gatgaggtag aagggcagac
     2101 acgggggtct gcccctacca gatgaaggaa tcgtgcccat cttgtagggg cggacctctg
     2161 tgtccgccct ttctccctta cagcagacgg cgtcgatagg caccgggggc actcgccctc
     2221 acccaagttg gcgcccgctc accgccgtgc tatcttcccg cgagcgtatt cgtgaacgag
     2281 cccgagggag gcatcgcatg gcgagccgcg aggagttgcg ccagcgcatc gtcgacgcta
     2341 tcgaagcgaa ccgcgagcgg attgtcgcgg tcggcgagga gatccggctc aacccggaga
     2401 tcggctacga ggaggtcaag gcgtcgcggc tgctggccgc caacatccgc gaccacggct
     2461 acgaagtcga gaaaccggcc ggcgggctgg agacggcctt cgtggccgcc cggcacgggc
     2521 gcgccgaggg gccggtggtg gcggtgctgg ccgaatacga cgccctgccc ggcatcggcc
     2581 acggctgcgg ccacaacctg atcgccgcct cgggcttggc cgccgccatc ggcctgaacg
     2641 ccgtcatgga cgagatcgac ggtatcttcg aggtgatcgg caccccggcc gaggagagcg
     2701 gcggcggcaa gatccagctg atcgacgctg gcgtcttcgc cgacgtcgac gcctcgctga
     2761 tggtccacca cggcggcaac aacaccgacg ccgccagcga ctaccccaac ggccgcagcc
     2821 tggcggtggc ccacgtcgac tacgagttct tcggcaagcc ggcgcacgcg gccagcgacc
     2881 cctggaacgg caacaatgcc gtcaacgcgg tggtggcact cttcaacggc atcgacgccc
     2941 tgcgccagca catccacatg gagtcgcgta tccacggcat catcacgcat ggcggcgacg
     3001 cgccgaatgt cgtgcccaat cacgccgccg ccaagttctt catccgggcc gagacgatgg
     3061 cgtacatgca ggagctggtc gacaaggtgg ccaggatcgc cgagggtgcg gccctgatga
     3121 ccggctgcga actgaagatc accgagggcg aggtctgcta cgacatgcgc cccagctacg
     3181 tggtcggcgg gcgctacaag gagcacatgc gggagatggg gctggatatc aacccgcgcc
     3241 cggccggccg caaggcctcg accgacttcg gcaacgtcag ctacctgatg ccatcggtca
     3301 ccgggcgctt cgccatcagc cacgagccga tccccggcca ctcgcagcag gtggtggacg
     3361 cctcg
//
LOCUS       DASUBI010000545         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_626708, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000545 DASUBI010000000
VERSION     DASUBI010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626708"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1548)
                     /locus_tag="VFU72_10490"
     CDS             complement(46..1548)
                     /locus_tag="VFU72_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU5423958.1"
                     /translation="MRIAIGGISHESSTFATVPTALPDFEQRSLNEGDLLLQKYRGTH
                     THLGGFIDAARDCAYEVVPTLFASATPGGPVTAEATEVLTRKLAEGLRQALASGPLDG
                     VLLALHGAMVSEISDDGETYILRHVREVVGPDLPVIITLDLHGNITQEMVDLVTVPVA
                     YDEYPHTDPYERGYEAGILMARVVRGGLRPTAAIVKLPLMPALQRMHSHAEPMLGVKA
                     LAWTIEHEFGVLNVSYLPGFPWSDIAPAGFSVIVTTDNDPAQARAAAERLAGYVWSRR
                     EEFAVQPTPVDEAVRGAMTAKGPVVLADIGDNPGGGAPCDGTVLLESLLRLGAKDAVV
                     VPITDPEAVQQAIAAGVGGRVSLQLGGKTDTMHGAPLAVTGRVVRLSDGRFVHKGPMS
                     TGVEHNMGPTAVLELEGAQGGRVLVVVSTHRYQPIDLEVLRSQGIEPSQQQMIAVKSS
                     VHFRAAFTPIASEIVEVDTPGLTSPKLENFDYRKVTRPLYPLDRDAEWQG"
     gene            1799..>2617
                     /locus_tag="VFU72_10495"
     CDS             1799..>2617
                     /locus_tag="VFU72_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5423959.1"
                     /translation="MEQPTKQLRGASSRRTFLGRGLALGAGVAGAGVLSSSLTVASAS
                     AHPASGSAITKGDVAILQFLSALEILESDLWQQYNELGGIPDDEVPGGSVSQPYFDAL
                     AVLDEDMAQYIHDNTEDEISHFTFLNAYLASQGADPVSLERFRTLPGSQATGAVKNAP
                     RLTNLMQLTVDTSWWTRYRSRTGNPDLGDTFPQAVPGLSMGQFPAIPRSDDDLMPGDH
                     IQAIANTASFHFAFIEQGGTSLYPSLAQRVTNPEVLRILLAIGPTETMHFQTWQD"
BASE COUNT          393 a          943 c          874 g          407 t
ORIGIN      
        1 cccagtattg ggagaggctc cgtccgggcc gggggtgagg gcgcgtcacc cctgccactc
       61 ggcgtcgcgg tcgagcgggt agagcgggcg cgtgaccttg cggtagtcga aattctccag
      121 cttggggctg gtcaggcccg gcgtgtcgac ctcgacgatc tcgctggcga tcggggtgaa
      181 ggcggcgcgg aagtgcaccg acgacttcac cgcgatcatc tgctgctggc tcggctcgat
      241 cccctggctg cgcagcacct ccagatcgat cggctggtag cggtgcgtgc tgacgaccac
      301 cagcacccgg ccgccctgcg cgccctccag ctccagcacc gccgtcggcc ccatgttgtg
      361 ctcgaccccg gtgctcatcg gccctttgtg cacgaagcgc ccgtcgctca ggcgcaccac
      421 gcggcccgtc accgccagcg gcgcgccgtg catggtgtcg gtcttgccgc ccagttgcag
      481 gctgacccgg ccgcccacgc ccgccgcgat cgcctgctgg accgcctcgg ggtcggtgat
      541 cggcaccacg acggcatcct tcgcgcccag ccgcagcagc gactccaaga gcaccgtgcc
      601 gtcgcagggc gcgccgccac cggggttgtc gccgatgtcg gccaggacca ctggtccttt
      661 ggccgtcatc gccccgcgca cggcctcgtc gaccggcgtc ggctgcaccg cgaactcttc
      721 gcgtcgcgac cagacgtagc cggccagccg ctcggcggcc gcccgcgcct gcgccgggtc
      781 attgtcggtg gtgacgatga cgctgaagcc ggccggagcg atgtcggacc aggggaagcc
      841 ggggaggtag ctgacgttga gcacgccgaa ctcgtgctcg atcgtccagg ccagcgcctt
      901 gaccccgagc atcggctcgg cgtggctgtg catgcgctgc agcgccggca tcagcggcag
      961 cttgacgatc gccgccgtcg gccgcagccc gccgcgcacc acgcgcgcca tcaggatgcc
     1021 ggcctcgtag ccgcgctcgt aggggtcggt gtgcgggtac tcgtcgtagg ccaccggcac
     1081 ggtaaccaga tcgaccatct cctgggtgat gttgccgtgc agatcgagcg tgatgatcac
     1141 cggcaggtcg gggccgacca cctcgcgcac gtggcgcagg atgtaggtct cgccgtcgtc
     1201 gctgatctcg gagaccatgg cgccgtgcag cgccagcagc acgccgtcga gcgggccgct
     1261 ggcgagcgcc tggcgcagtc cctcggccag cttgcgggtc agcacctcgg tcgcctcggc
     1321 ggtcaccggg ccgccggggg tggccgaggc gaagagggtc ggcaccacct cgtaggcgca
     1381 gtcgcgcgcg gcgtcgatga agccgccgag gtgcgtgtgg gtgccgcggt acttctgcag
     1441 cagcagatcg ccctcgttca ggctgcgctg ctcaaagtcg gggagcgcgg tcggcaccgt
     1501 agcgaaggtg ctgctctcgt gcgagatgcc gccgatcgcg atacgcatgg gcttctcctt
     1561 ctcagattct cagcatccgg ctgcggcgtg gctgccggcc ccatactagc ccaagaatcc
     1621 gcatcgtggc cggctcaatc catctgtgcg cgtgccgcgt acatggatca tccgcgctcc
     1681 ggcatccggt tgaggagctc caccgggcgc ggcgggaagc atgacgggac aatccgatcg
     1741 aggtccgtgc acgtccccga ctcgccttcg tcccgaactg gacacggaga acacgatcat
     1801 ggagcagccg accaagcaat tgcgtggcgc cagtagccgg cgcacgttcc tgggcagggg
     1861 actcgcgcta ggtgccgggg tagccggcgc cggtgtattg agcagcagcc tcaccgtcgc
     1921 ctccgcctct gcccatcctg ccagcggcag cgcgatcacc aagggcgacg tggccattct
     1981 gcaattcctg tctgccctgg agatcctgga gtccgatctc tggcagcagt acaacgagct
     2041 gggcggcatc cccgacgatg aggtgcccgg cggcagcgtc agccagccct acttcgacgc
     2101 gctcgcggtg ctcgacgagg acatggcgca gtacatccac gacaacaccg aggacgagat
     2161 cagccacttc acctttctga acgcctacct ggcgtcgcaa ggggcggacc cggtcagcct
     2221 tgagcgcttc cgcaccctcc cgggcagcca ggcgaccggc gccgtgaaga acgccccgcg
     2281 ccttaccaac ctgatgcagc tgacggtcga taccagctgg tggacgcgct atcgcagccg
     2341 caccggcaac cccgacctgg gcgatacctt cccccaggcg gtccccggct tgagcatggg
     2401 gcagtttccg gccattcccc gcagcgacga cgacctcatg cccggcgatc acatccaggc
     2461 gatcgccaac acggccagct tccacttcgc cttcatcgag cagggcggca ccagtctcta
     2521 cccgtcgctg gcgcagcggg tcacgaaccc cgaggtgctg cgcatcctgc tcgcgatcgg
     2581 cccgaccgag acgatgcact tccagacctg gcaggac
//
LOCUS       DASUBI010000546         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_110673, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000546 DASUBI010000000
VERSION     DASUBI010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110673"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFU72_10500"
     CDS             complement(<1..320)
                     /locus_tag="VFU72_10500"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423960.1"
                     /translation="MPSATGERETWEVDGRRIEAVNLDKLYWPETGLTKGDVLAYYRT
                     VAPAMLPFLRDRPLTLVAYANNGRGRYLRERPAKAPGWLPSVEYHLETKDVVRQEPVV
                     DDAAG"
     gene            complement(331..1533)
                     /locus_tag="VFU72_10505"
     CDS             complement(331..1533)
                     /locus_tag="VFU72_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5423961.1"
                     /translation="MNKHRIDWGPLLAPRAAAIHASVYPKFGALPEHPDVINFKGGVP
                     AAECLPIGRLRQSICHAWEVEPDVLGYAESEGYLGLRGLIAERMARRGAPVSPHEVLV
                     TNGSQQGLELIAKLLLEPGDRVVVEGPTYFGALQIFDIYQATYDVVPMDEEGIDPVAL
                     EATLQREPRPKLIYLIPTFQNPAGVSLSPERRRAVVELSRRYNVPIVEDDPYGELWFE
                     RDLGPLRALDPDVLYLGTFSKTLAPALRMGWLVAPPELMQRLIDSKESVDIQSDRVMQ
                     RAVVETAGAGWLDRHLERVRPLYRARRDHLLARLAEEMPPGTRWTRPGGGFFVWVTLP
                     AGRSADELLQRAAAAGVQYYPGSSFYPNTCQRPTFRLGYTTLPEERITEGLARLGTVA
                     RAQANASG"
     gene            complement(1526..2272)
                     /gene="alaXM"
                     /locus_tag="VFU72_10510"
     CDS             complement(1526..2272)
                     /gene="alaXM"
                     /locus_tag="VFU72_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaXM"
                     /protein_id="HEU5423962.1"
                     /translation="MTDLLYLADSYLREFDAVVTEVTPEGGVILDQTAFYPGGGGQPN
                     DTGLLRAGGQTWQVSKVAKAGGDAVHTLEGGEPPSPGTPVHGAVDWERRYQLMRTHTA
                     MHVLCGTIFREYGALVTGGNMALDKARMDFELEDLSPERVAHIEELANGAIARGLPVS
                     WRSLPRAEAFAIPDLIRTKINLLPDSIREVRVVEIAGLDLQADGGTHVRNTRDVGGIK
                     VVGTRSKGRINKRLEIVLTEGPLGGQGSRE"
     gene            2424..3275
                     /locus_tag="VFU72_10515"
     CDS             2424..3275
                     /locus_tag="VFU72_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEU5423963.1"
                     /translation="MARFEWPDGKRCAVALTFDADGETFPLVMDRERGHERLTLISTG
                     IYGPEVGVPRLVEMLEQFGLKATFFIPGFTAELHSEMIELLTANGHEIGHHGYLHERP
                     DTLSDEQEEAVLVRGLEALQRLTGKRPVGYRSPAWELKPTSPALLKRYGFLYDTSLMG
                     NDIPYYVATDQGNLVELPIQWILDDYAHYSMDSGGISSPQKVFEVWSSEFAGYHRYHG
                     CYVLTMHPFVSGRPSRVLLLERLIQYINEFEGVWWATLQEIADYCVRSGQLGVWTPPD
                     LKTLAGR"
BASE COUNT          514 a         1174 c         1172 g          532 t
ORIGIN      
        1 ccggccgcgt cgtcgacgac gggctcctgg cgcaccacgt ctttggtctc caggtggtac
       61 tcgaccgagg ggagccagcc cggcgctttg gccggccgct cgcgcaggta gcggccccgg
      121 ccgttgttgg cgtaggccac cagcgtcagc ggacggtccc gcaggaaggg cagcatggcc
      181 ggtgcgaccg tgcggtagta ggcgagcacg tcgcccttgg tgagaccggt ttcgggccag
      241 tagagcttgt cgaggttgac cgcttcgatg cggcgcccgt cgacctccca ggtctctcgt
      301 tcacctgtcg cggacggcat cacccagctc ctagccggaa gcgtttgcct gggcgcgggc
      361 taccgtaccc agccgcgcca gtccctcggt gatgcgctcc tccggcaagg tcgtatagcc
      421 caggcggaag gtgggacgct ggcaggtgtt ggggtagaag gaggagccgg gatagtactg
      481 caccccggcg gcggccgcgc gctgcagcag ctcgtcggcg ctccgcccag cgggcagcgt
      541 gacccagacg aagaagccgc cgccgggccg ggtccagcgc gtaccgggcg gcatctcctc
      601 ggccagccgt gccaacaggt ggtcgcgccg ggcgcggtag agcgggcgca cccgctccag
      661 gtgccggtcg agccagccag ccccggcggt ctcgaccacc gcgcgctgca tgacccggtc
      721 gctctggata tcgacgcttt ctttggagtc gatcaggcgc tgcatcagct ccggcggcgc
      781 gaccagccag cccatgcgca gtgccggcgc cagggtcttg gagaaggtgc cgaggtagag
      841 cacgtcgggg tcgagggcac gcagcggccc cagatcgcgc tcgaaccaga gctcgccgta
      901 ggggtcatcc tccacgatcg gcacgttgta gcgtcgcgac agctcgacga ccgcgcggcg
      961 ccgctccggg ctgagcgaga ccccggccgg gttctggaag gtcgggatca ggtagatcag
     1021 cttggggcgc ggctcgcgct gcagggttgc ttccagcgcc accggatcga tgccttcttc
     1081 gtccatcggc accacgtcgt aggtggcctg gtagatgtcg aagatctgca gcgcgccgaa
     1141 gtaggtcggg ccctcgacca ccacgcggtc acccggctcc aggagcagct tggcgatcag
     1201 ctccaacccc tgctgcgagc cgttggtcac cagcacctca tgcggcgaga ccggcgcgcc
     1261 gcggcgggcc atgcgctcgg cgatcagccc gcgcagcccg aggtagcctt cggattcggc
     1321 gtagccgagc acgtccggct cgacctccca ggcgtggcag atcgactggc gcaggcgccc
     1381 gatcggcagg cactcggcgg cgggcacgcc ccccttgaag ttgatcacgt cggggtgctc
     1441 gggcagcgca ccgaacttgg ggtagaccga ggcgtggatc gccgcggcgc gtggcgccag
     1501 cagcggcccc cagtcgatcc ggtgcttatt cacgcgatcc ctgcccgccc agcggtccct
     1561 cggtcaagac aatctccagt cgcttgttga tgcggccctt gctgcgcgtg ccgacgacct
     1621 tgataccgcc cacgtcgcgc gtgttgcgca cgtgcgtgcc gccgtcggcc tgcaggtcga
     1681 gccccgcgat ctcgacgacg cgcacctcgc ggatcgaatc cggcagcagg ttgatcttgg
     1741 tgcggatcag gtcggggatg gcgaaggcct ccgcgcgcgg cagcgagcgc caggagaccg
     1801 gcagcccccg ggcgatggcc ccgttggcca gttcctcgat gtgcgccacc cgctccggcg
     1861 agagatcctc cagttcgaag tccatgcgcg ccttgtccag cgccatgttg ccgccggtca
     1921 ccagcgcgcc gtactcgcgg aagatcgtgc cgcagaggac gtgcatggcg gtatgggtgc
     1981 gcatgagctg gtagcgccgc tcccagtcga ccgcgccgtg caccggcgta cccggcgagg
     2041 gcggctcgcc gccctccagc gtgtggacgg catcaccgcc ggccttggcc accttactca
     2101 cctgccaggt ctgccctccg gcgcgcagga ggccagtgtc gttgggctgc ccgccgccgc
     2161 cggggtagaa ggcggtctga tccaggatga cgccgccctc gggcgtcacc tcggtgacca
     2221 cggcgtcgaa ctcacgcagg tagctatcgg ccagataaag caagtcggtc acacagcggc
     2281 tcctcgctga ccacggtcgc gcatacgccg tcaatgatag cagggaggac agccggctcg
     2341 ctcttgaccg cgcgcgctcg cgggggtatc agcggagccg gacgcgcccg aagcgacggg
     2401 cgcggcgcgg agaggagcgc aacatggcac ggttcgagtg gccggacggc aagcgctgcg
     2461 ccgtggcgct gacgttcgac gcggacggcg agacgtttcc actggtgatg gaccgcgagc
     2521 gcgggcacga gcgcctgacg ctgatctcga ccggcatcta tgggccggag gtcggcgtgc
     2581 cgcgcctggt ggagatgctg gagcaattcg gcctcaaggc gaccttcttc atccccggct
     2641 tcaccgccga gctgcactcc gagatgatcg agctcttgac cgccaacggg cacgagattg
     2701 gccatcacgg ctatctccac gagcggcccg acacgctgtc ggacgagcag gaggaagccg
     2761 tgctggtgcg cggcctcgaa gcgctgcagc ggctgaccgg caagcggccg gtcggctacc
     2821 gctcgccggc ctgggagctg aagccgacct cgccggcgct gctcaagcgc tacggctttc
     2881 tttacgacac cagcctgatg ggcaacgaca tcccctacta cgtcgccacc gatcaaggga
     2941 acctggtcga gttgccgatc cagtggatcc tggacgacta cgcgcactac tccatggaca
     3001 gcggcggcat ctccagcccg cagaaggtgt tcgaggtctg gtcgagcgag ttcgccggct
     3061 accaccgcta ccacggctgc tatgtcctga ccatgcaccc cttcgtcagc ggccggccct
     3121 cgcgcgtgct gctcttggag cgcttgattc agtacatcaa cgagttcgaa ggtgtctggt
     3181 gggccacgct gcaggagatc gccgattact gcgtgcgctc cggccagctc ggcgtctgga
     3241 ccccgcccga cctgaagacg ctggccgggc gctagccggt ccgtgagcga agcgcgggtg
     3301 agccacacgt cctacccaat agcccacctg tcctttggtc cagtaatcct cctgtccgca
     3361 ggctgcactg aaggcagtcc tttcggtcga tg
//
LOCUS       DASUBI010000547         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_410958, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000547 DASUBI010000000
VERSION     DASUBI010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410958"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU72_10520"
     CDS             <1..274
                     /locus_tag="VFU72_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423964.1"
                     /translation="NFPDLGLQVFDPRDGIGAPTTLCYPEASSVGAHWAGPFPYDHGP
                     IAVYAPQHTHPHPSFSPDGTRVVFTSDRTGQAQVYEALLPMKEQER"
     gene            271..1548
                     /locus_tag="VFU72_10525"
     CDS             271..1548
                     /locus_tag="VFU72_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5423965.1"
                     /translation="MSLDRPLDVALIGTGNRSQTTYQPLFASLQPWVRLVAVCDPVRE
                     NADAFAAAVGVPAFYSLRDLVQARICEAALVVTPIDGHSAISCYLSAHGIHHLVETSM
                     ASSLVQGRRMVDAAHEHNVTLRIAENFFRFPFDRIAKVVADSGFIGAVKRLTCFHDHL
                     GYHNNSRWIVFYGAYPESVQAFSHTMPTAPHHQAAARAHRHHESETFNIRLYTFPDNR
                     LVTDLAGNIKGMLGRYPRPGYTEIDGARGTIVQQATNDWQGDLAVQQETENWQAKAEV
                     RYCTDAELERSGQADQIFPIVNCSEEGNWTSTYVNLPSGRIEYVNPYRPSGRVLINRD
                     YYGAAVMGHIVDFAKAVRGVAPSEYTEQDALMAMMMEMGGRESARRDGARIALPLAED
                     LEVDELSLAAQRQKYGVDPLDVEAMLAVSYPKP"
     gene            1625..2692
                     /locus_tag="VFU72_10530"
     CDS             1625..2692
                     /locus_tag="VFU72_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5423966.1"
                     /translation="MPEHELRCIVVGMGGISKTMLRELAHKPWYRTVAVVDVRDEALA
                     LAGTTLGLPATALFTDLDRALASVHADAVLINTPSELHYAQARAAIAAGRHVLVAKPV
                     TNDYEQAVELVDLAVAHGVTHSVGQQMRYNRHYTAVRRFVAAGALGAVEAAYFLNSKP
                     RPEPANLARMPQPSLYENACHHFDAFLGIFADQAPEWVSCDGFIPSWSPYVGPCMVNA
                     LIRFSGGLHLLYHGGFSARAPLYEFRLEGAKGVLSCHGIHMSNDTMRYEFAPALGAFA
                     PATIDEGIPARSPWQPFFDLWHDYVLGGPEPSFSGRNNLPVFALLSAAIASVEEGNPV
                     EVAGNPRYERAFRQPAGAAHD"
BASE COUNT          485 a          943 c          900 g          451 t
ORIGIN      
        1 gaactttccc gacctcggcc tgcaagtgtt cgacccgcgc gacggcatcg gcgcaccgac
       61 gactctctgc tatcccgagg cgtcgagcgt cggcgcgcac tgggccggac cattccccta
      121 cgaccacggc ccgatcgcgg tctacgcgcc gcagcatacc cacccgcacc cctcattcag
      181 cccggacggc acgcgcgtcg tctttacgag cgaccgcacc ggccaggcgc aggtatacga
      241 ggccctgtta ccgatgaagg agcaggaacg atgagcctgg atcgtccgct cgacgtcgcc
      301 ctgatcggca ccggcaaccg gtcgcaaacg acctaccagc cgttgtttgc gtcactccag
      361 ccctgggtcc ggctggtcgc cgtctgcgac ccggtgcgcg agaacgccga tgccttcgcc
      421 gccgcggtgg gcgtgccggc cttctactcg ctgcgcgatc tcgtgcaggc ccggatctgc
      481 gaggccgcgc tcgttgtcac cccgattgac ggacacagcg cgatttcgtg ctatctctcg
      541 gcgcacggga ttcaccacct ggtggagacg agcatggcga gctcgctggt gcagggccgc
      601 cgaatggttg acgcggcgca cgagcacaat gtcacgctgc gcatcgcgga aaacttcttc
      661 cgcttcccct tcgaccgcat cgctaaggtc gtcgccgaca gcggctttat cggtgcggtg
      721 aagcgcctga cgtgcttcca cgaccatctc ggctaccaca acaactcgcg ctggattgtc
      781 ttctatggcg cgtatcccga gtcggtgcaa gcattctccc ataccatgcc gaccgcccct
      841 caccaccagg ccgcggcgcg cgctcaccgt caccacgagt cggagacgtt caacatccgg
      901 ctctatacct tccccgacaa ccggctggtg accgatctcg caggcaatat caagggaatg
      961 ctggggcgct acccgcggcc gggctatacg gagatcgatg gcgcgcgcgg gaccattgtg
     1021 cagcaggcga cgaatgactg gcagggcgat ctggcggtcc agcaggaaac cgaaaactgg
     1081 caggccaaag ccgaggtgcg ctactgcacg gatgcagagc tggagcgcag cggacaggcc
     1141 gaccagatct tcccgatcgt caactgctca gaggagggca actggacctc gacgtacgtc
     1201 aacctgccga gcgggcggat cgagtacgtg aatccctacc ggccgagcgg gcgtgtcctg
     1261 atcaaccggg actattacgg agcggcggtg atgggccaca tcgtggactt cgccaaggct
     1321 gtgcgcgggg tcgcgccgtc ggaatatacc gaacaggacg cgctgatggc gatgatgatg
     1381 gagatgggcg gacgcgaatc ggcgcggcgt gacggggcac ggatcgcgct gccgctggcc
     1441 gaagatctcg aagtcgacga gttatccctc gcggcgcaac gacagaagta cggcgttgac
     1501 ccactcgacg tcgaggcgat gctggcggtg agttatccca agccgtgaac ggccccctca
     1561 ctccctcgcc tgtaaggcgg gcgagggggg agagggtgag ggtcattgag aaggagtctt
     1621 ccgtatgcct gagcacgaac tgcggtgcat cgtcgttggc atgggcggca tcagtaagac
     1681 gatgctgcgc gaactggcgc acaagccgtg gtaccgcacc gtcgcggtgg tggacgtgcg
     1741 tgacgaagcg ctggcgctgg ccggcacgac gctcggtctg cccgccacgg cactcttcac
     1801 cgacctcgac cgcgcgctgg cgagcgtaca cgccgacgcc gtgctgatca atacgccgtc
     1861 agagctgcac tatgcgcagg cgcgggccgc gattgcggcc ggacggcatg tgcttgtcgc
     1921 caagccggtc acgaacgact acgagcaggc ggtcgaactg gtggacctgg cggtggcgca
     1981 cggggtgacg cacagcgtcg gccagcagat gcgctacaac cggcactaca ccgccgtgcg
     2041 gcgcttcgtc gcggcgggcg cactcggcgc ggtcgaagcg gcctatttcc taaacagcaa
     2101 accccggccg gaaccggcga acctggcgcg catgccccag ccctcgctct acgagaacgc
     2161 ctgccaccac ttcgacgctt ttttgggcat cttcgccgac caggcgccgg aatgggtctc
     2221 gtgcgacggc ttcatcccct cgtggtcgcc ctacgtcggg ccgtgcatgg tcaacgcgct
     2281 catccggttc agcggcgggc tgcacctcct gtatcacggc ggcttctctg cgcgcgcccc
     2341 gctctacgag ttccggctgg aaggagccaa gggcgtgctc agttgccacg gcatccacat
     2401 gtcgaacgac acgatgcgct acgagttcgc gccggcactc ggcgcgttcg cgcccgccac
     2461 gattgacgag ggtataccgg cgcgcagccc ctggcagccg ttcttcgacc tctggcacga
     2521 ctatgtgctc ggcgggccgg aaccatcctt cagcgggcga aacaatctgc cggtcttcgc
     2581 gctcttgagc gccgcgatcg cgtcggtgga agagggcaac cctgtcgaag tcgccgggaa
     2641 tccgcgctac gagcgcgcgt tccgccagcc agcaggagcc gcgcatgact gaacacgcgt
     2701 acgaactccg ccatgaatcg ctcagcccca cgcacatcgc cgggcgattc gtcgtcgagc
     2761 gcgccaccgg gcggctgct
//
LOCUS       DASUBI010000548         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_928074, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000548 DASUBI010000000
VERSION     DASUBI010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928074"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..775
                     /locus_tag="VFU72_10535"
     CDS             173..775
                     /locus_tag="VFU72_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5423967.1"
                     /translation="MAQRSEGRHEPRAMEAPVESGPPEPVGTGGERTLLPYGVTLSSS
                     TAVLHPERTLEFLGFDHLAIRVADVRRAESFYHEFFEMDVVLRARRFEDNWEVMPSTF
                     DYTEGLTSGYYPEVVQLRNGALALVLINAGRGAVLAEPRLGHVGLRVPPDTLTALRGI
                     ALVRNFAVEEDARTAFRFTDPYGITWHLTTGHEAEAGASS"
     gene            772..1233
                     /locus_tag="VFU72_10540"
     CDS             772..1233
                     /locus_tag="VFU72_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5423968.1"
                     /translation="MTDIYHELLEALAETPVRIQELAERAGAAPKPAVGPEGWGPAAI
                     IAHLADTERILRGRIEQMLAREAPYLKSFDPDALAAERGYADRELRAALEDFATERAE
                     TIQRLMNLPLKGWERSGIHDEQGEVSVEDVAEQLVDHDAAHRAQLETALAG"
     gene            1484..2827
                     /locus_tag="VFU72_10545"
     CDS             1484..2827
                     /locus_tag="VFU72_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEU5423969.1"
                     /translation="MPIFDPQMETMPREQLRRLQNERLAQIVRYAYERVPFYHQALDE
                     AGVRPDEIGSVDDITRLPFTRKSDLRDNYPFGLLAVPREDLLRVHASSGTTGKPTVVS
                     YTAADIDLFAEVNARSLAAAGAAPGSMLHNAYGYGLFTGGLGLHYGGEKLGMIVVPVS
                     GGVTERQITLILDFRPDMIACTPSYGQTLAGEFAKRGVSPEEISLKYAVLGAEPWTQT
                     IREHVDAGLGLRSSNIYGLSEIIGPGVSQECVDERAGAHIWEDYFYPEVVDPGGDEPL
                     PEGQAGELVLTTLTKEAMPLLRYRTGDVTSLSYEPCSCGRTHVRMDRILGRTDDMLIV
                     RGINLYPTELELILRRIKGVVPHYQLVVTRDGALDEAELKVEVTPALFLSAGEAALSG
                     ELSAAEEPLRRLRQEIVHRVRETLGFRISATLMAPGSVPRSEGGKLRRVVDKRQL"
     gene            2838..3260
                     /locus_tag="VFU72_10550"
     CDS             2838..3260
                     /locus_tag="VFU72_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5423970.1"
                     /translation="MRPIPPGYQASFETRVTPEMLVDFEELGAVHPVYATYWLAKHME
                     LVGRKIILPFLEAGEEGIGYAVTVRHLAPALLGMRVRTVGEYLGSEGNRVRVRCRAEN
                     ELGEIIGEGETVQVILPLEKLHERFAALERRWEAAREG"
     gene            3265..3576
                     /locus_tag="VFU72_10555"
     CDS             3265..3576
                     /locus_tag="VFU72_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEU5423971.1"
                     /translation="MPKVKVVALYRKPEDVAAFMQHYQNVHLPLVRQTPGLERLEVDR
                     VTASASGGEPEYFLITGMIYPDQETFRAAVRSDANKAVMQDVQQFAGNLITVLIAEVL
                     E"
     gene            3573..>4361
                     /locus_tag="VFU72_10560"
     CDS             3573..>4361
                     /locus_tag="VFU72_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5423972.1"
                     /translation="MSGVGAGAGEYRSIVVETREGGVGLIRLNRPAQLNALNRETMTE
                     VARAVRAFDDDEAVRAIVITGDERAFAAGADINEFAGMSVPGMIEEYRFEQWEALRQA
                     RTPLIAAVSGWALGGGCELAMLCDMIVASETARFGQPEINLGLIPGAGGTQRLTRQVG
                     KYLAMELVLTGRSLDAREAQQHGLVNRIVPKELYLEEALKLAREVASKSPVAVRLAKD
                     AVRKAQDTTLEVGLSYERHNFYLLFGTEDHTEGIQAFLEKRKPEW"
BASE COUNT          705 a         1483 c         1547 g          626 t
ORIGIN      
        1 cacctcctgg ctcggcgcgg atcaagcacc ttcaccgata ccagacaggt gccttcctgt
       61 cacctcatga ataatccagg ctagtcgcgc cggccgcgct gggctcgcat ctcggcaaga
      121 agggtgcgag aatggcgggg tgagatgggt ggagaccgag caggaggagc gcatggcaca
      181 gcggagcgag gggcggcacg agccacgagc gatggaggcg ccggtcgagt cggggccgcc
      241 ggagccggtc gggaccggcg gcgagcgcac gctcctcccc tacggcgtga cgctctcctc
      301 cagcacggcg gtgctgcacc ccgagcgcac gctcgagttc ctgggcttcg accacctggc
      361 gatccgggtg gccgacgtgc gccgggccga gtccttctac cacgagttct tcgagatgga
      421 cgtggtgctg cgcgcccgcc gcttcgagga caactgggag gtcatgccca gcaccttcga
      481 ctacaccgag gggctgacca gcggctacta ccccgaggtg gtgcagctgc gcaacggcgc
      541 gctggcgctg gtgctgatca acgccggccg gggcgcggtg ctggccgagc cgcgcctggg
      601 ccacgtcggg ctgcgcgtcc ccccggacac gctgaccgcg ctgcgcggca tcgccttggt
      661 gcgcaacttc gcggtcgagg aggacgcccg caccgccttc cgcttcaccg acccctacgg
      721 catcacctgg cacctgacga ccggccatga ggcagaggca ggagcgtcgt cgtgaccgac
      781 atctaccacg aattgctcga agcgctggcc gagacaccgg tacgtatcca ggaactggcc
      841 gagcgcgcgg gcgccgcgcc gaagcccgcg gtcggccccg agggctgggg gccggcggcg
      901 atcatcgccc acctggccga caccgagcgc atcctgcgcg ggcgcatcga gcagatgctg
      961 gctcgcgagg cgccctatct caagagcttc gaccccgacg cgctggccgc cgagcgcggc
     1021 tacgcggaca gggagctgcg cgcggcgctg gaggacttcg ccaccgagcg cgccgagacg
     1081 atccagcggc tgatgaactt gcccctgaag ggctgggagc gcagcggcat ccacgacgag
     1141 cagggcgagg tcagcgtcga ggacgtggcc gagcaactgg tcgaccacga cgcggcccac
     1201 cgggcgcagc tcgagaccgc gctggcgggc tgacgacgag ggctgggtag tagggcaaga
     1261 cggatccttc gacaagctca ggatgacagt cttcattttc agtcctgtcg gatcacgcga
     1321 atcgtgctcg tacgccacac ctctaaccct gtcatcctga gcgcactcga aggatccgtc
     1381 ctcacacggc actcatgcca ggtcgttcgc catcccgccc tcagaccgga aaggagccct
     1441 gtgacccgaa ccccttgaac ccccccggaa aggagcggtt gagatgccca tcttcgaccc
     1501 tcagatggag acgatgcccc gcgagcagct gcgccggctg cagaacgagc ggctcgccca
     1561 gatcgtgcgc tacgcctacg agcgcgtccc cttctaccac caggcgctcg acgaggccgg
     1621 cgtgcggccg gacgagatcg gctcggtcga cgacatcacc cggctcccct tcacccgcaa
     1681 gagcgacctg cgcgacaact accccttcgg cctgctggcc gtgccgcgcg aagaccttct
     1741 gcgggtccac gcctccagcg gcaccaccgg caagccgacc gtggtcagct acaccgccgc
     1801 cgacatcgat ctcttcgccg aggtcaacgc gcgcagcctg gccgcggccg gcgccgcgcc
     1861 cggctcgatg ctccacaacg cctacggcta cggcctcttc accggcggcc tgggtctgca
     1921 ctatggcggc gagaagctgg gcatgatcgt ggtgccggtc tccggcggtg tgaccgagcg
     1981 ccagatcacc ctgatcctcg acttccggcc cgacatgatc gcctgcaccc cctcgtatgg
     2041 gcaaacgctg gccggcgagt tcgccaaacg cggcgtgtct cccgaggaga tcagtctcaa
     2101 atacgccgtg ctcggcgccg agccctggac gcagaccatc cgcgagcacg tcgacgccgg
     2161 gctgggcctc cgctccagca acatctacgg gttatccgag atcatcgggc cgggcgtcag
     2221 ccaggagtgt gtcgatgagc gcgcgggggc gcacatctgg gaggattatt tctacccgga
     2281 ggtggtggac cccggcggcg acgagccgct gccggagggg caggccggcg agctggtgct
     2341 gaccaccctg accaaggagg ccatgccgct gctgcgctat cgcaccggcg acgtcacctc
     2401 gctctcctac gagccctgct cctgcggccg cacccacgtg cggatggacc gcatcctggg
     2461 gcgcaccgac gatatgctga tcgtgcgcgg catcaacctc tacccgaccg agctcgagct
     2521 gatcctgcgc cggatcaagg gggtggtgcc ccactatcag ctcgtcgtca cccgcgacgg
     2581 cgcgctggac gaggcggagc tgaaggtcga ggttacgccg gcgctctttc tgtcggcggg
     2641 cgaggcggcc ctctccggcg aattgtcggc cgccgaggag ccgttgcgcc gcctgcgcca
     2701 ggagatcgtc caccgcgtcc gtgagacact cggtttccgc atcagcgcca ccctgatggc
     2761 gccggggagc gtgccgcgca gcgagggcgg caagctgcgc cgggtggtcg ataagcggca
     2821 gctctagggg ccgcggcgtg cgcccgatcc cgcccggcta ccaggccagc ttcgagacgc
     2881 gggtcacgcc cgagatgctg gtcgatttcg aggagctggg ggcggtgcat ccggtctacg
     2941 ccacctactg gctggccaag cacatggagc tggtcggccg caagatcatc ctgccctttc
     3001 tggaggcggg cgaggagggg atcggctacg ccgtgacggt gcgccacctg gcccccgcgc
     3061 tgctcgggat gcgcgtgcgt actgtcgggg agtatctggg cagcgagggc aaccgcgtgc
     3121 gggtgcgctg ccgggccgag aacgagctgg gggaaatcat cggcgagggg gagaccgtgc
     3181 aggtgattct gccgttggag aagcttcacg agcgcttcgc cgcgctcgag cgccgctggg
     3241 aagcggccag agagggttga agagatgccg aaggtgaagg tcgtcgcgct ctaccgcaag
     3301 ccggaggatg tcgcggcgtt catgcagcac tatcagaacg tgcacctgcc gctggtgcgg
     3361 cagacgccgg ggttggagcg gctggaggtt gaccgcgtca ccgcctccgc ctccggcggc
     3421 gagccggagt acttcctgat caccgggatg atctatcccg atcaggagac cttccgcgcg
     3481 gccgtgcgct ccgacgccaa caaggcggtg atgcaggacg tgcagcagtt cgccggcaat
     3541 ctgatcaccg tgctgatcgc ggaggtgctc gagtgagcgg ggtaggggcg ggcgcgggcg
     3601 agtaccgctc gatcgtggtc gagacgcggg agggcggcgt cgggctgatc cggctcaacc
     3661 ggccggcgca gctcaacgcg ctcaaccgcg agacgatgac cgaggtcgcg cgggccgtgc
     3721 gcgccttcga tgacgacgag gccgtgcgcg ccatcgtgat caccggcgac gagcgcgcct
     3781 tcgccgccgg ggccgacatc aacgagttcg ccggtatgag cgtgccgggc atgatcgagg
     3841 agtaccgctt cgagcagtgg gaggcgctgc ggcaggcgcg cacgccgctg atcgccgccg
     3901 tgtccggctg ggcgctgggc ggcggctgcg agctggcgat gctctgcgac atgatcgtgg
     3961 ccagcgagac ggcgcgcttc ggccagccgg agatcaacct gggcctgata cccggcgccg
     4021 gtggcaccca gcgcctgacg cgccaggtgg gcaagtacct ggcgatggag ctggtgctca
     4081 ccgggcgctc gctggacgcg cgcgaggcac agcagcacgg cctggtaaac cggatcgtgc
     4141 ccaaggagct gtatctggag gaggcgctca agctggcgcg tgaggtggcc tccaagtcgc
     4201 cggtggcggt gcggctggcc aaggacgcgg tgcgcaaggc gcaggacacc acgctggagg
     4261 tcggcctgag ctacgagcgg cacaacttct acctgctctt cggcaccgag gaccacaccg
     4321 agggcatcca ggccttcctg gagaagcgca agcccgagtg g
//
LOCUS       DASUBI010000549         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_164640, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000549 DASUBI010000000
VERSION     DASUBI010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164640"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFU72_10565"
     CDS             complement(<1..376)
                     /locus_tag="VFU72_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5423973.1"
                     /translation="MTNSNGTPRVLITVASKHGATRGIADAIAEELRRRGLDAEVRAA
                     DEVRGIQGYDAVVLGSAVYMGKWLPAARDLADREREVLAGLPVWLFSSGPLGHEHPQP
                     EGEPAQLDQLLAQTNARGHRIFA"
     gene            complement(414..1313)
                     /locus_tag="VFU72_10570"
     CDS             complement(414..1313)
                     /locus_tag="VFU72_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423974.1"
                     /translation="MAISKPSPSPSARGRPAPAPRTRTVARRLGARFALPTWLSIPLV
                     AVAASAAAFGVFDPSIFRDPAMTAGNARGTALVVLVLALPVLIGSIILTWRGSLRAQL
                     VWLGALGYILYNSFVFAFVVHFNALFLLYVAMLSLAFWSLLTLLRRLDPEVFRAHAAP
                     GLPLRFIAGVLVVLTLLFFATWMRDIVPALIDHSTPAGLEGTGMLTNPFQVMDLSVVF
                     ALQLLTAVWLWRRRAWGFLLAPALLVMQTIESVSVASDQTFGHLADSTQSLAAVPLFA
                     VLALVVLVPTVIILRGIQEKATG"
     gene            1526..1879
                     /locus_tag="VFU72_10575"
     CDS             1526..1879
                     /locus_tag="VFU72_10575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5423975.1"
                     /translation="MSDDQERGGIRRHVTLKQAAGVPVPEGNLAAPVLRHGSLEVEYY
                     APRGEDQQRPHTRDEVYVVIAGQGTFLNGTERHPFGPGDVIFVPAGVVHRFEEFSDDF
                     ATWVLFYGPEGGEAP"
     gene            1941..2336
                     /locus_tag="VFU72_10580"
     CDS             1941..2336
                     /locus_tag="VFU72_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423976.1"
                     /translation="MGQPAAIVAELVQEFVGNAHGNLERVRELLRAQPALVNAAWDWG
                     GGDWETALGAAAHMGRRDIAAELLAHGARLDLFAAAMLGQVEIVRAILGAFPELRRAS
                     GPHGIPLLAHAQAGGEEAAAVVRLLQDEP"
     gene            complement(2333..>2581)
                     /locus_tag="VFU72_10585"
     CDS             complement(2333..>2581)
                     /locus_tag="VFU72_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEU5423977.1"
                     /translation="AEGLAEIPGIQIDPEAVETNILFFDVRETGQTAEQIAAGLRERG
                     VRINGSNYRFRAVTHLDVSREDIDRTIEAMTEVVMARR"
BASE COUNT          419 a          889 c          932 g          341 t
ORIGIN      
        1 ccgcgaagat gcggtggccg cgggcgttcg tctgggcgag caattgatcg agctgcgccg
       61 gctcgccctc cggctgcggg tgctcgtggc cgagcgggcc gctgctgaag agccagaccg
      121 gcagcccggc cagcacctcc cgctcgcggt cggccagatc gcgcgccgcc ggcagccact
      181 tgcccatgta gaccgcgctg ccgaggacaa ccgcgtcgta gccctggatg ccccgcacct
      241 cgtcggccgc gcgcacctcg gcgtcgagcc cgcgccgccg caattcctcg gcgatggcgt
      301 ccgcgatgcc gcgcgtcgca ccgtgcttac tggccaccgt gatcagcacc ctcggtgtgc
      361 cgttgctgtt cgtcattgct tccctccgat tatccggtac cttcgcgccg ccctcaccca
      421 gtcgctttct cctgaatacc ccgcagtatg atcacggtcg ggaccagcac gaccagcgcc
      481 agcaccgcga ataacggcac ggccgccagc gactgggtcg agtcggccag gtggccgaag
      541 gtctggtcgg aggccacgct gaccgactcg atcgtctgca tcaccagcag cgcgggcgcg
      601 aggagaaagc cccaggcccg gcggcgccag agccagacgg cggtcagcaa ctgcagcgcg
      661 aacacgacgc tcaggtccat cacctggaag gggttggtca gcatgcccgt cccctctaga
      721 ccggccggcg tgctgtggtc gatcaaggcc ggcacgatgt cgcgcatcca ggtggcgaag
      781 aagaggaggg tcaggacgac cagcaccccg gcgatgaagc gcagcggcag gccgggcgcg
      841 gcgtgggcgc ggaagacctc cggatcaagc cggcgcagca gcgtcaagag tgaccagaac
      901 gccagcgaga gcatcgccac atagagcagg aagagcgcgt tgaagtggac cacgaaggcg
      961 aatacgaacg agttgtagag gatgtagccc agcgcgccca gccagaccag ttgcgcccgc
     1021 agcgagccgc gccaggtcag gatgatcgag ccgatcagca ccggcagcgc cagcaccagg
     1081 accaccagcg cggtgccgcg cgcattcccg gcggtcatgg ccggatcgcg gaagatggac
     1141 gggtcgaaga cgccgaacgc ggccgcgctc gccgccaccg ccaccagcgg gatcgacagc
     1201 caggtgggta gcgcgaagcg cgcaccaagc cgccgcgcga cggtgcgcgt ccggggcgct
     1261 ggcgccggcc gcccccgtgc cgagggtgag ggtgagggtt tcgagattgc catcgggtga
     1321 tctccctatc gtgctcttgc ggatctgcag gccacccgag ccgagcgggc caggccggcc
     1381 tcctcgcagg gcatgtgccg gacgacaacc cggcactccg tagcctagcc cggccgccgt
     1441 ccagcgagcg tcacgcccgg cggggcgccg ttacaatggc gcgacagtcg ccgggcagcg
     1501 aacgccggca gcgaggagcg cacgagtgag tgacgatcag gagcgaggcg gaatacgccg
     1561 ccatgtcacg ctcaagcagg cggcgggggt gcctgtgccg gaaggcaatc tggcggcgcc
     1621 ggtgctgcgc cacggcagtc tggaggtcga gtactacgcc ccgcgcggtg aagaccagca
     1681 gcggccccac acgcgcgacg aggtctacgt ggtcatcgcc gggcagggca cgtttctcaa
     1741 tggcacggag cgccacccct tcggccccgg cgacgtgatc ttcgtcccgg cgggggtcgt
     1801 tcaccgcttc gaggagttca gtgacgactt cgccacctgg gtgctgttct acggtccgga
     1861 gggcggcgag gcgccctgac aaccaggaac catctcgcgc gtcaaagaga agcaaaaacg
     1921 gtatgagcca aggaggagcg atggggcagc cggcggcgat cgtggccgag ctggtgcagg
     1981 aattcgtcgg gaacgcgcac ggtaacctgg agcgggtgcg cgagctattg cgggcgcagc
     2041 cggctctggt caacgccgcc tgggactggg gtggcggcga ctgggagacg gcgctgggcg
     2101 cggcggcgca catggggcgg cgcgacatcg ccgcggagct gctggcgcat ggcgcgcggc
     2161 tcgacctctt cgccgccgcc atgctcggcc aggtcgagat cgtgcgcgcc atcctcggcg
     2221 ccttcccaga gctgcgccgg gccagcggcc cgcacggcat cccgctgctg gcgcacgcgc
     2281 aggccggcgg cgaggaggcc gccgccgtgg tgcgcctgct gcaagacgag ccctagcgcc
     2341 gcgccatcac cacctcggtc atcgcctcga tggtgcggtc gatgtcctcg cggctgacat
     2401 caaggtgcgt gacggcgcgg aagcggtagt tgctgccgtt gatgcgcacg ccgcgctcgc
     2461 gcagcccggc ggcgatctgc tcggcggtct ggccggtctc gcgcacgtcg aagaagagga
     2521 tgttggtctc gaccgcctcg gggtcgatct ggatgcccgg gatctcggcc aggccctccg
     2581 c
//
LOCUS       DASUBI010000550         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_652334, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000550 DASUBI010000000
VERSION     DASUBI010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652334"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFU72_10590"
     CDS             <1..810
                     /locus_tag="VFU72_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423978.1"
                     /translation="GPFICLEKGTWAELIDLYARADGRIYTSPEMAAWFDTVLPPAAL
                     GGLSLVLDGDLPKREWFLDARSPRISAVDGELHTVVPGRPIGLHPWSVGELADAGIHL
                     HFYGDFTHGQWRAWIEKALRLAPRHLHLHPNVDQENWVTEFSRYDAGWLHVFSSENAG
                     DIRRANWDDLNYPARLPTLVAAGLPLLQHDNRGAIVATQALVRELDIGIFFSETTELP
                     ALLRDQASMSRLRANVWAQREQFSFDSHADRLVDFFREVITRHEESRGSRR"
     gene            933..2039
                     /gene="ychF"
                     /locus_tag="VFU72_10595"
     CDS             933..2039
                     /gene="ychF"
                     /locus_tag="VFU72_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5423979.1"
                     /translation="MPLQLVIIGFPLSGKTTVFNALTQSEAPTGGFSTGEEEPNLATV
                     KVPDQRLEVLTRMFNPRREVPADVQYYDVAGIAQGIHEQGLSGRLLGYLSQAAALVQV
                     VRAFENPAVPAPSGSVDPLRDIEAMNLELTFSDLGLIEKRIPRLEAGIPKLRGAEREA
                     NERELAALIRLREALEAGLPIREVVLDAEEERLLRGFGFLTGKPLLILLNVGEDMLGA
                     QSAALVAEARERFARPGVTIEALAGKIEEEIARLDPDDAALFLADLGIEETSRERVIR
                     ASYDLLGLISFFTVGPDETRAWTIHRGTPAVEAAGVIHSDIQRGFIRAEVVSYEDLVQ
                     TGGMPEARKAGRLRLEGKQYVVQDGDICHFLFNV"
     gene            complement(2088..>2647)
                     /locus_tag="VFU72_10600"
     CDS             complement(2088..>2647)
                     /locus_tag="VFU72_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423980.1"
                     /translation="GPPPAQPTAVAAPVIDAAAGETPATIPTPEPTADAQPVVSAPQP
                     TTVPVSPTAPANTPAATEVAAEPSARTPTPASTPTPPTPTPGPTPVTAPTTQTELASE
                     PVDAKQVKVQSDAPAVQPWHQGNGPWKATPAPVQPPQQHAAAGPSKQLADPKAPTPTQ
                     PAPQTGHDSPHAAPGWGHGKSKGQK"
BASE COUNT          363 a          911 c          951 g          422 t
ORIGIN      
        1 ggtccgttca tctgtctgga gaaaggcacc tgggcggagc tgatcgatct ctatgcccgg
       61 gccgacggcc gcatctacac gtcccccgaa atggcggcgt ggttcgacac ggtgcttcct
      121 ccggccgcgc tgggcgggct ctcgctggtc ctcgatggcg acctgccgaa gcgagagtgg
      181 ttcctggatg cacgctcgcc gcggatctcg gcggtggacg gcgagctgca cacggtcgta
      241 ccgggcaggc cgatcggcct gcacccctgg tccgtcggcg agctcgctga cgccggtatc
      301 cacctgcact tctacggcga cttcactcac ggtcagtggc gcgcctggat cgagaaggcg
      361 ctgcgcctcg cgccccggca cctgcacctg caccccaacg tcgatcagga gaactgggtc
      421 accgagttct cgcgctacga cgccggctgg ctgcacgtct tctccagcga gaacgcgggc
      481 gacattcgcc gcgccaactg ggacgacctc aactacccgg cccgcttgcc aacgctggtc
      541 gcggcgggtc tgccgctcct ccagcacgac aatcggggcg cgatcgtcgc cacccaggca
      601 ctcgtccgcg agctggacat cgggatcttc ttctcggaga cgactgagct gcccgccctc
      661 ctgcgcgacc aggctagcat gagcaggctg agggccaacg tctgggcgca gcgcgagcaa
      721 ttcagcttcg actcccacgc cgatcgcctg gtcgacttct tccgcgaggt catcacgcga
      781 cacgaggaaa gccgcggcag ccgtcgctga ggccggggca gacgccggtg cgccgggcgc
      841 ggctctgcag gagacggcac agggcgatac aataggcggt cgccgcgagt cgctgccggg
      901 cgcgccggca gcgcgatcgc cacggaggac gcatgcccct gcaactggtc atcatcggct
      961 ttccgctcag cggcaagacc acggtcttca acgcgctcac ccagtcggag gcgcccaccg
     1021 gcggcttctc caccggcgag gaggagccga acctggcgac cgtcaaggtg cccgaccagc
     1081 ggctcgaggt gctgacacgc atgttcaacc cgcgccgcga ggtgccggcc gacgtgcagt
     1141 actacgacgt ggccggcatc gcccagggca tccatgagca ggggctgagc ggccggctgc
     1201 tgggctacct cagccaggcg gccgcgctgg tgcaggtggt gcgcgccttc gagaacccgg
     1261 ccgtgcccgc gccctccggc tccgtcgacc cgctgcgtga tatcgaggcg atgaacctgg
     1321 agctgacgtt ctccgacctc ggcctgatcg agaagcgcat cccccggcta gaggccggca
     1381 tccccaagct gcgcggcgcc gagcgcgagg ccaacgagcg cgagctggcg gcgctgattc
     1441 gactgcgcga agcgctggag gccgggctgc cgatccgcga ggtggtgctc gatgccgagg
     1501 aggagcggct gctgcgcggc ttcggcttcc tgaccggaaa gccgctcctg atcctgctca
     1561 atgtcggcga ggatatgctc ggcgcccagt ccgcggcgct ggtagccgag gcgcgcgagc
     1621 gcttcgcgcg tcccggtgtg acgatcgagg cgctggccgg caagatcgag gaggagatcg
     1681 cgcggcttga cccggacgac gccgctctct tcctggccga cctgggcatc gaagagacca
     1741 gccgcgagcg cgtcatccgc gcctcctacg acctgctcgg cctgatctcc ttcttcaccg
     1801 tcggcccgga tgaaacccgc gcctggacga tccaccgtgg cacgccggcc gtcgaagcgg
     1861 ccggggtgat ccactccgac atccagcgcg gcttcatccg cgccgaggtg gtcagctacg
     1921 aagacctggt acagaccggg ggcatgcccg aggcgcgcaa ggccggccgc ctgcgcctgg
     1981 agggcaagca gtacgtggtc caggacggcg acatctgcca ctttctgttc aacgtgtagt
     2041 gttcggcgct atcgacttta ttgggtgccg gtactggccg tcgcgatcta tttttgcccc
     2101 ttgcttttgc cgtgacccca gccgggcgcg gcgtgcgggg agtcatggcc ggtttgcggc
     2161 gcgggctgcg tgggcgtggg agccttcggg tcggccaact gcttgctggg gccagcggcg
     2221 gcgtgctgtt ggggcggctg gacgggcgcg ggtgtcgcct tccagggtcc gttaccctgg
     2281 tgccacggct gcaccgctgg ggcgtcgctc tgcactttga cctgcttggc gtcgaccggc
     2341 tccgaggcca actcggtctg ggtcgtgggc gcggtcaccg gagtcggccc gggtgtcggc
     2401 gtcggaggtg tcggcgtgga cgccggtgtt ggcgtccgcg ccgacggttc ggcggcgacc
     2461 tcggttgcag ccggagtgtt cgccggcgcg gtcggggaaa ccggcacggt agtcggctgc
     2521 ggcgcgctca cgaccggctg cgcgtcagcg gtcggctcgg gcgtgggaat cgtcgccggt
     2581 gtctcgcccg cggccgcatc gatgaccggc gccgcgacgg cggtcggctg ggcgggcggg
     2641 ggcccgg
//
LOCUS       DASUBI010000551         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_819421, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000551 DASUBI010000000
VERSION     DASUBI010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819421"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFU72_10605"
     CDS             <1..937
                     /locus_tag="VFU72_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423981.1"
                     /translation="YSHVMPLAAGDVRPEGIFLTLDRTTPIGRFREDPSFQAGEMSFS
                     RYLIGLSQGDRSIVGLPIFAMHGFRQRCFFVRRGSSLRSLADLAGKHIGTNGWPDTGN
                     TWSRAALRTAGVDLGSITWSVGPVDAPLDDEATARAQAALPTGVTQLAAGQTLAGQLA
                     SGQLDAIMCPWPPAGFYDPSSPIVRLFPAYQAAEGDYYRHTGIYPAHHIVGLRRDLFE
                     RHPWVARSLFRAFDESRLQSQADNLLLADASPWVQADIERAMRLMGTDWQPNGIEPNQ
                     HVIAALCEEEYAQGLIAQPLEPATVFADFKQAAEA"
     gene            complement(947..1723)
                     /locus_tag="VFU72_10610"
     CDS             complement(947..1723)
                     /locus_tag="VFU72_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEU5423982.1"
                     /translation="MRRGVAGWRRALVAPLVLLLLSGAVIILLNRPAGDPLPPAGPVI
                     WSANQESRDLSEWTAGQKGESVFNTGTGRVEVSQDVAHGGRFSLKMSIQGAAGQTEGA
                     RAFRWSENQEEAYYSVWFYIPQTYKPVQWWSIFQFKSVTTESDPMWMVDIDNRPDGSM
                     YLYLWDAIDGRSFTPRHQIDLPVGRWFLLTAYFRRERDGTGRIALWQGTTLLYDIDHV
                     RTVTGDSVHWGICNYTDNIAPPDVTIYADDAEIRTVLAVR"
     gene            complement(1824..>2151)
                     /locus_tag="VFU72_10615"
     CDS             complement(1824..>2151)
                     /locus_tag="VFU72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU5423983.1"
                     /translation="NPRDARGRNAPPQRAELAHCSGFLRSTIELVDSCLVAALGASAL
                     RALALVTPHELVLARDVGRPVRWGERWLVALYHPSPRAQLHRPIARQRDDFRALGRFL
                     ATLPKM"
BASE COUNT          330 a          754 c          713 g          354 t
ORIGIN      
        1 ttacagccac gtcatgccgc tggcggccgg cgacgtgcgg ccggagggga tctttctgac
       61 cctcgaccgc acgactccga tcgggcgttt tcgcgaggat ccgtcttttc aggccggcga
      121 gatgtcgttc agccgctacc tgatcgggct gtcgcagggt gatcggagca tcgtggggct
      181 gccgatcttc gcgatgcacg gcttccgcca gcgctgcttc ttcgtgcgac gcggctcgtc
      241 gctgcgctcc ttggccgatc tggccggcaa gcacatcggc accaacggct ggcccgatac
      301 cggcaacacc tggagccgtg ccgccctgcg cacggcgggt gtcgatttgg gtagcatcac
      361 ctggtccgtc ggcccggttg acgccccact tgacgatgaa gccacggcgc gggcgcaggc
      421 cgcgttgcct accggcgtga cccagctcgc ggcgggtcaa accctggctg ggcagctcgc
      481 ctcaggccag ctggacgcga taatgtgccc ctggccgcca gccggcttct acgacccgag
      541 ctcgcccatc gtgcgcctct tccccgccta tcaggccgcc gagggggatt actaccgtca
      601 caccggcatc tacccggcgc accacatcgt cggtctgcgc cgcgatctgt tcgagcgtca
      661 tccctgggtc gcccgcagcc tcttccgggc cttcgatgag agccgcctgc agtcgcaggc
      721 cgacaatctg ctgctggccg acgcctcgcc ctgggtgcag gccgacatcg agcgcgctat
      781 gcgcctcatg ggcaccgact ggcagccgaa cggcatcgag cccaaccagc acgtgatcgc
      841 cgcgctctgc gaagaggagt acgcccaggg cctgatcgcc cagccgctcg aaccggccac
      901 cgtctttgcg gacttcaagc aggccgccga ggcttgagca gcagcgctag cgcacggcga
      961 ggacggtgcg gatctcggca tcgtcggcgt agatggtgac gtcgggcggc gcgatgttgt
     1021 cggtgtagtt gcagatgccc cagtgtacac tgtcgccggt cacggttcgt acgtgatcga
     1081 tgtcgtagag cagcgtcgtg ccttgccaca gcgcgatgcg gccggtaccg tcgcgctcgc
     1141 gccggaagta ggccgtgagc aggaaccaac ggccaaccgg cagatcgatc tggtggcgag
     1201 gggtgaagct gcgtccgtcg atcgcgtccc agaggtagag gtacatgctc ccgtcgggac
     1261 ggttgtcgat gtcgaccatc cacatcgggt cgctctcggt cgtgaccgac ttgaactgga
     1321 agatcgacca ccactgcacc ggcttgtacg tctgagggat atagaaccag acgctgtagt
     1381 acgcctcctc ctggttctcg gaccagcgga aggcgcgcgc cccctcggtc tgacccgccg
     1441 ctccctggat gctcattttc agcgagaatc ggccaccgtg cgccacatcc tgcgacacct
     1501 cgacgcgtcc ggtgccggtg ttgaaaaccg actcgccctt ctgtcccgcg gtccactccg
     1561 agagatccct gctctcctgg ttcgcggacc agatcaccgg tcccgccggg ggcagcggat
     1621 cgccggcggg ccggttcagc aggatgatga cggccccgct gaggagcaga agcacgagcg
     1681 gcgcgaccag ggctcgccgc caacccgcga cgcctcttct catcgctcgc ctcccgcccc
     1741 gccgataccg ctctcctgac accctacgct cacctgggac cagcggacag ggagattcgt
     1801 cgtggcgggc cgggccgatg gtcctacatc tttgggagcg tggcgaggaa gcggccgagc
     1861 gcgcgaaagt cgtcgcgttg ccgggcaatc gggcgatgga gctgggcgcg tgggctgggg
     1921 tggtagagcg ccaccagcca gcgctcaccc cagcgaaccg gccgtcccac gtcgcgcgcc
     1981 agtaccagct cgtgcggcgt caccagcgcc agcgcccgca atgcgctggc accgagcgcc
     2041 gccaccaggc acgaatcgac cagctcgatg gtcgagcgca ggaagccgga gcagtgcgcc
     2101 agctccgccc gctggggcgg cgcgttgcgg ccgcgtgcat cacgcgggtt g
//
LOCUS       DASUBI010000552         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1258979, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000552 DASUBI010000000
VERSION     DASUBI010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258979"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1582)
                     /gene="rpoN"
                     /locus_tag="VFU72_10620"
     CDS             complement(158..1582)
                     /gene="rpoN"
                     /locus_tag="VFU72_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU5423984.1"
                     /translation="MAGMDIGYELTPEMRVWISPSLIEANYILSLSRMELQDVIKQEL
                     EANPALEMEDKQICPVCGGVIEGSFCPTCLINQANEAERSEESYEDYPETLYSSISRT
                     RDDGDDFDPMTLVASELTLPEQILSDVATLCHNGDLAVAEYLVGSLDDRGFITLDLDD
                     VARRFRKTRDYVERVVGFIQSVAPIGVGARDLRECLLLQIRYLRDIGVEVPQFVEPIV
                     EGFLDEFGSHKFGYIAKELNISHEDVEDAREFIRTHLNPYPLQSQEARYWKTPLRSPY
                     VAPDVTVNLRDDSLHVEVVDTRHFHLRLSPLYDQIAHEIGRSRTRGNYTDGERQHIRE
                     FVNRAKLFMSNINQRRETLYKISRCIVDLQEDFLRGGVRELRPLTRAIVAQQVGVHES
                     TVSRATANKFVMLPNRKVIPFSDFFTPSLSVKDVIKELIEKENNPLTDRKICDLLAKQ
                     GIRIARRTVAKYRAELNILPSTMR"
     gene            1906..3048
                     /locus_tag="VFU72_10625"
     CDS             1906..3048
                     /locus_tag="VFU72_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5423985.1"
                     /translation="MGSGIGLLTIRGIKIKLHPSIVLALAWVVYHWDVSARHGWAGAG
                     FGFVLLLGIFACVVGHELAHGFLAQRYGLAVHDIVLLPIGGVARIEHLPLSARREAMI
                     ALAGPLLNLLVALGLTPLLLAIVLTRHLASPLAILSLAGEIGFSGLIFQFWFANLMLA
                     LFNLLPAFPMDGGRIFRAAVAGLSNRLTATQVASLVGQGFALLIVVVGLLAHDLMLPL
                     LALFIVASAYVESRLTRLEDVLRKLPVAQFAVWDMGGIDPEAPLSFALRGGARDVAVV
                     DRGIVVGMLWREQILSRLSGAAQLRACEVMDTTVTPMSVDDSVFSVHQQMLARRQPAI
                     PITAGGRYRGVFTAERLAHVYRHLQRTSTRRLSYRDLAEALGLIGR"
     gene            complement(3164..>4564)
                     /locus_tag="VFU72_10630"
     CDS             complement(3164..>4564)
                     /locus_tag="VFU72_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5423986.1"
                     /translation="STPDLPPAGSVVPATFAQNVTWSEEAGWHWVYEAFDGSQRFTSE
                     QYDVSFGVPQPVPFYVATRVRTLGANDACADCVMLDNFQQGPPKILGGPDNPALKDAY
                     AHIKDGARQMIKGDSAAGNAPRWTKGSWYLNYDPTTGKFFSWTWIPEDWTAQALEQGL
                     VPKQPQGVLAATTTGPGGVRLFPLAGSYQITQPYGCVPANGGYPSPSSCPANRPSFHD
                     GVDLGAPAGTPIFAAASGTVIFAGIDSTASGNSKIVIQLDGANAAYQNVYLHWRKTFV
                     SVGMHVSAGQVIAEVGSVGYSTGPHLHFSVTQTGGGTVDPLAWLRASLPAPAANGHIN
                     TADAVGIMGWASLIQQAAAAHKVPAALLAAIMTVESDGNPRAISPQGAQGLMQVMPDE
                     FQRYGVPVEKWLDPATNIDTAARILSETLGQGGAVDLAVQRYFGIGCDALGTCTDVYL
                     SRVQQWLDFYQKNFPS"
BASE COUNT          685 a         1471 c         1573 g          835 t
ORIGIN      
        1 acggccagtc ggatgctcac tttgaaggtg cgtacgaacg cggaatctat cgcggttact
       61 ggccgaagta tagcgcgcgg caagcgcgcg ggcacaacga acggccccgg tgataaacac
      121 accagggccg gcctcgttca cgttcgtatg cgcccggcta gcgcatggtg ctgggcagga
      181 tgttcagctc ggcgcggtac ttggcgaccg tacggcgggc gatgcggata ccctgcttgg
      241 ccaggaggtc gcagatcttg cggtcggtca gggggttgtt ctccttctcg atcagctcct
      301 tgatcacgtc cttgacgctg agcgaggggg tgaagaagtc gctgaacggg atgaccttgc
      361 ggttgggcag catcacgaac ttgttggcgg tcgcacggct gaccgtcgac tcgtgcacgc
      421 cgacctgctg ggccacgatg gcgcgcgtca gcgggcgcag ctcacgcacg ccgccgcgca
      481 ggaagtcctc ctggagatcg acgatgcaac gggagatctt gtagagcgtc tcgcgccgct
      541 ggttgatgtt tgacatgaag agcttggcgc ggttgacgaa ctcgcggatg tgctgccgct
      601 cgccgtcggt gtagttgccg cgggtgcggc tgcgcccgat ctcgtgcgcg atctgatcgt
      661 agagtgggct gagacgcagg tggaagtggc gcgtgtcgac gacctcgacg tgcaggctgt
      721 cgtcgcgcag gttcaccgtc acgtcggggg cgacgtaggg cgaacgcagt ggcgtcttcc
      781 agtagcgcgc ctcctggctc tgcagcgggt aggggttgag gtgcgtgcgg atgaactcgc
      841 gcgcatcctc gacatcttcg tggctgatat tcaactcttt ggcgatgtac ccaaatttat
      901 gcgagccgaa ctcatccaga aaaccctcga cgatcggctc gacgaactgc ggcacctcga
      961 cgccgatatc gcgcaggtag cggatctgca gcagcaggca ctcgcgcagg tcgcgcgcgc
     1021 cgacgccgat cggcgccacc gattggatga agccgaccac gcgctcgacg tagtcccgcg
     1081 tcttgcggaa gcgccgcgcg acgtcatcca ggtcgagggt gatgaagccg cgatcgtcga
     1141 gcgagccgac caggtattcc gccaccgcca ggtcgccgtt gtggcagagc gtggcgacat
     1201 cggacaggat ctgctcgggg agcgtcagct ccgaggccac cagcgtcata gggtcgaagt
     1261 cgtcgccatc gtcgcgcgtg cggctgatgc tggagtagag cgtctcgggg taatcctcgt
     1321 aggactcctc gctacgctcc gcctcgttcg cctggttgat caagcaggtc gggcagaagg
     1381 aaccctcaat gaccccgcca cagaccgggc agatctgctt gtcctccatt tcgagcgcgg
     1441 gattcgcctc gagctcctgc ttgattacat cctgcagctc catacgcgag aggctcagaa
     1501 tgtagttggc ctcaatcagg cttggtgaaa tccaaacccg catctcgggc gtgagctcgt
     1561 atccgatatc cattcccgcc attcgcggac tcctccgtga atacgctcga ttccgctcaa
     1621 tacagcgccg ccaggttcaa tctcgggcca ggtcggcgga gatgcatcgc cgctgctcat
     1681 ccccagtcta cccggcgggc gaaacgttgg caaggttctt gctagaacaa cgcgatctca
     1741 gttgcgctgt gacgctcgcc ccggcaacgc gccgtcgatg actctcgttc atcgctcgct
     1801 gcaccagggc taacgcacag cttgtgccac aggacacaat cgggaagcgc cggccgatta
     1861 ggcggtccgc tgccgggctg ctatgatgcg gctgggaggg ttgctttggg ctcaggtatt
     1921 ggcctgctca cgattcgtgg gattaagatc aagctccacc ccagcatcgt cctggcgctg
     1981 gcctgggtcg tctatcactg ggacgtctcg gcgcgtcacg gctgggccgg tgccgggttc
     2041 ggcttcgtgc tgctactcgg gatctttgcc tgcgtggtgg gtcacgaact ggcgcacggc
     2101 ttcctggcgc agcgctacgg gctggcggtg cacgacatcg tcctgctgcc gatcggcggc
     2161 gtggcccgca tcgaacacct gccgctctcc gcgcggcgcg aggcgatgat cgcgctggcc
     2221 gggccgctgc tcaacctgct cgtcgcgctc ggcctgaccc cgctgttgct ggccatcgtg
     2281 ttgacgcggc acctggcgtc gccgttggcg atcctgtcgc tggcgggcga gatcggcttc
     2341 agcgggctga tcttccaatt ctggttcgcc aacctgatgc tggcgctctt caacctgctg
     2401 ccggccttcc cgatggacgg tggccgcatc tttcgcgcgg cggtcgccgg cctcagcaat
     2461 cgcctcacgg ccacccaggt cgcctcgctg gtcgggcaag gtttcgccct gctgatcgtg
     2521 gtcgtcgggc tgctggcgca cgatctgatg ctgccgctgc tggcgctctt catcgtcgca
     2581 tcggcctatg tcgagtcgcg cctgacgcgc ctggaggatg tcttgcgcaa gctcccggtg
     2641 gcgcaattcg cggtctggga catgggcgga atcgaccctg aagcgccgct cagcttcgcc
     2701 ctgcgcgggg gggcgcgcga cgtggcggtg gtggaccggg ggatcgtggt gggcatgctc
     2761 tggcgcgagc agatcctcag ccgtttgagc ggggccgcgc agctcagagc ctgcgaggtg
     2821 atggacacca cggtcacgcc gatgagcgtg gacgactcgg tcttctcggt acaccagcag
     2881 atgctggccc ggcgccagcc ggcgatcccg atcaccgccg gcgggcgcta ccgcggcgtc
     2941 ttcaccgccg agcgcctggc acacgtctac cgccacctgc agcgcacctc gacccgccgc
     3001 ctcagctacc gcgacctcgc cgaggcgctc ggcctcatcg gccgctaagg tggcctccca
     3061 cagccttggg tgccgcgtgc gtccgggatg gcgaccatcg tgttcgaggg gcggcgcccc
     3121 tctcccagta ttgggagtgg ggttggggtg tgggatctcc gccctacgac gggaagttct
     3181 tctggtagaa gtcgagccac tgctgcacgc gggacaggta gacgtcggtg caggtgccga
     3241 gcgcgtcgca gccgatgccg aagtagcgct gcaccgccag gtcgaccgcg ccgccctggc
     3301 cgagcgtctc cgagagaatc cgcgccgcgg tgtcgatgtt ggtggccgga tcgagccatt
     3361 tctcgaccgg aacgccgtag cgctggaact cgtcgggcat cacctgcatc agcccctgcg
     3421 ccccctgtgg cgagatggcc cgcgggttgc catcggactc gaccgtcatg atcgccgcca
     3481 gcagcgctgc cggcaccttg tgcgcggcgg cggcctgctg gatcagcgag gcccagccca
     3541 tgatgccgac cgcgtcggcg gtgttgatgt gcccgttggc ggccggcgcc gggagcgagg
     3601 cccgcagcca ggcgagcgga tcgaccgtgc cgccgccggt ctgcgtcacc gagaagtgca
     3661 ggtgtgggcc ggtcgagtag ccgaccgagc cgacctcggc gatcacctgc cccgcgctga
     3721 cgtgcatgcc cacgctgacg aaggtcttgc gccagtgcag gtagacgttc tgataggccg
     3781 cgttggcgcc gtcgagctgg atcacgatct tggagttgcc actggcggtt gagtcgatgc
     3841 cggcgaagat gaccgtgccg ctggcggcgg cgaagatcgg tgtgccggcc ggagcgccga
     3901 gatcgacgcc gtcatggaag ctcggccggt tggccggaca cgagctgggg ctggggtagc
     3961 cgccgttggc cggcacgcag ccgtagggct gggtaatctg gtacgagccg gccagcggga
     4021 agaggcgcac cccacctggt ccagtcgtcg tcgccgcgag gacgccctgc ggctgcttcg
     4081 gcacgagccc ctgctccagc gcctgcgcgg tccagtcctc gggtatccag gtccaggaga
     4141 agaacttgcc cgtcgtgggg tcgtagttca ggtaccatga gcccttggtc cagcgcggcg
     4201 cattgcccgc ggccgaatcc cccttgatca tctgacgggc gccgtccttg atgtgggcgt
     4261 aggcgtcctt cagcgcgggg ttgtccggtc cgccgaggat cttgggcggt ccctgctgga
     4321 agttgtcgag catcacacag tcggcacagg cgtcgttcgc gccgagcgtg cgcacccgcg
     4381 tggccacgta gaagggcacc ggctgcggca cgccgaagga gacgtcatat tgctcgctgg
     4441 tgaagcgctg gctgccgtcg aaggcctcat agacccagtg ccagccggcc tcctcggacc
     4501 aggtcacatt ctgcgcgaag gtggccggca cgaccgagcc ggctggaggc aagtcgggtg
     4561 tgct
//
LOCUS       DASUBI010000553         5542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_796596, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000553 DASUBI010000000
VERSION     DASUBI010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796596"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1212)
                     /locus_tag="VFU72_10635"
     CDS             complement(16..1212)
                     /locus_tag="VFU72_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5423987.1"
                     /translation="MSMLEMKPSRRAAGLPVSPIRRLAPLAEAARARGTRVLHLNIGQ
                     PDLAPPATITQALAEAGGEALVYAPSRGLPDVLDAWQAYYRRHGIEVEAGDILVTFGA
                     SEAISLALLSTLDPGDDVLLPEPFYAPYKGSLAIAGCNLVPVPPGQGFAPPSAEEFRR
                     RLTPRTRAILITSPNNPTGTVFSRDELRALGRLAHDEGLFLLSDETYREIVFDGAAAP
                     SALSLPGLEEQVVVIDSLSKRFNVCGLRIGSLVTRNAGVMAAALELAELRLAVPVIDQ
                     RAAAAELRAGPEYVAGVVKTYRERVAAVVEALGAIPGVRVRPPGGAFYVVAGLPVADA
                     EAFAAWLLSDFALDGETVMLTPMADFYATPGQGRDEVRIACVYEPARLRRGVEILAAG
                     LAAYPG"
     gene            1352..3070
                     /gene="ggt"
                     /locus_tag="VFU72_10640"
     CDS             1352..3070
                     /gene="ggt"
                     /locus_tag="VFU72_10640"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5423988.1"
                     /translation="MTDVVSPLGAAGTAAGRRAATTIKQEAVASRGMVTSNHPLASLA
                     GTEMLLIGGNAVDAAVATLFALSVVEPMMVSIFGAGFLTIRLADGTITTIDDYATVPG
                     GARPDMFVPVAGSLDNDVVGGLNTTGYLAPATPGSLLGWATAVERYGRLPLAEVVAPA
                     VRYARRGFRVSPYLHHFIDLYRDDLARFPATAAVFLPGGDVPAIDAAIQRADYADTLE
                     RVGAHGPGWLYQGPLGDAVAADMARNGGLITGEDLAGYRIYERAPVRGSYRGYEIVSM
                     APASSGGTHIIQMLNILEGFDLAGMGFGSPQAVHLIAEAMKIAFADRFRYMADPERVS
                     VPVDWLTSKSYAAARRAEIEPARAQAYSAALAPDGEGASTTHCCAMDAEGNVVTTTQT
                     LNGGFGSKVTVPGTGMLLNNCMHLMDPTPGRTNSIAPGKRILSSMSPTIVLRDGRPLL
                     ALGTPGGVRIFGAVMQAIINVIDHGMTLQQAVEAPRLWDRGPVLELEEGFSELPALKA
                     DMERRGHTVQVVPKVAGGMNGILRDEQGLLHGAACWRADGAPIGFSGGDAQVDGAAGF
                     ATGVPR"
     gene            3104..3859
                     /locus_tag="VFU72_10645"
     CDS             3104..3859
                     /locus_tag="VFU72_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5423989.1"
                     /translation="MTDPRSIVRTPLLLPSSLEQYYEKTGPARRAADGDVILIATGWD
                     APDGNIAVVLGPAASERVFALADEFFGGEPYSVIVESESAAPVEASLRAAGWELDEDE
                     PALFLQPVPAPPPPPADLDIRLVTTEAELADFMEVSGTGQRWVPSLAAARDPGVALLV
                     GYVQGKPVATARINLLGRVGEINGVGTLPAYRRRGYGTAMTWAAIAEGVRRGCDAITL
                     SASEMGYSVYRRMGFVPVCRYRTYLPPGGQAEE"
     gene            3866..5383
                     /locus_tag="VFU72_10650"
     CDS             3866..5383
                     /locus_tag="VFU72_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5423990.1"
                     /translation="MSATNPTTEDTSLTRRLAELAAEHPDKPAIIFAPLAGDERAVSW
                     AELERESNQIARLLADRGVRGDSTVVGAVWNSPELILFTLAAWKLGALVLPLRAVLPP
                     RERDAILELARPALVLAEWESERWPVIRPEELAAASGYPADPLPAVPHPGKAIGSGGS
                     TGRPKLILDPAWNPVTARDNRRLGTRPGQVQLLAAPLYHNSPWLQSFNGLADDHTLVV
                     MEKFDATRAADLIERYRVSYAYLPPILMRRLALLPDFWERDFSSFDAMHSSAAVCPVW
                     LKQVWIDRLGAEKVYEVYGSAEGIGATIIRGDEWLQHRGSVGRSTNCEIRILDEDGSP
                     LPPGEVGEIYFRRVDGTTPSFHYEGAPPVRSTPDGFASLGDLGWLDADGYLYIADRRV
                     DMIVSGGANVYPPEVEAALSEHPGLADVAVIGVPDEEWGRRVHAVVQPHDPVNPPSVA
                     ELNAHTRERIAAWKAPKTYELVPELPRNEAGKIRRADLATARQNGWIEGMLRVRE"
BASE COUNT          762 a         2063 c         1957 g          760 t
ORIGIN      
        1 gggggtgagg gcccgtcagc ccggatacgc cgccaggccc gccgccagaa tctcgacgcc
       61 ccggcgcagg cgcgcgggct cgtagacgca ggcgatgcgc acctcgtcgc ggccctggcc
      121 tggcgtggcg tagaagtcgg ccatcggcgt cagcatgacg gtctcgccat cgagcgcgaa
      181 gtcgctgagc agccaggcgg cgaaggcctc ggcgtcggcc accggcagcc cggccaccac
      241 gtagaaggcg ccgccgggtg gccgcacccg cacgcccggg atcgcgccca gcgcctcgac
      301 gaccgccgcc acccgctcgc gataggtctt caccaccccg gcgacgtact ccggcccggc
      361 gcgcagctcg gcggccgccg cgcgctggtc gatgaccggc accgccagcc gcagctcggc
      421 cagctccagc gccgccgcca tcaccccggc gttgcgggtt accaggctgc cgatgcgcag
      481 cccacagacg ttgaagcgct tggagaggct gtcgatcacg accacctgct cctccagtcc
      541 cggcagcgag agcgcgctcg gcgcggccgc gccgtcgaag acgatctcgc ggtaggtctc
      601 gtcggagagc aggaagagcc cctcgtcgtg ggccagccgg ccgagcgcgc gcagctcgtc
      661 gcgcgagaag acggtgccgg tggggttgtt cgggctggtg atcaggatgg cgcgcgtgcg
      721 cggcgtcagg cggcgccgga actcctcggc cgagggcggg gcgaagccct gcccgggcgg
      781 caccggcacc aggttgcagc cggcgatcgc cagcgagccc ttgtaggggg cgtagaacgg
      841 ctccggcagg agcacatcgt cgcccgggtc gagcgtgctg agcagggcca gcgagatcgc
      901 ctcgctggcg ccgaacgtca ccaggatgtc gcccgcctcg acctcgatgc cgtggcggcg
      961 gtagtaggcc tgccaggcgt cgagcacgtc gggcagcccg cgcgagggtg cgtagaccag
     1021 cgcctcgccg ccggcctcgg ccagcgcctg cgtgatcgtc gccgggggcg cgagatccgg
     1081 ctggccgatg ttgagatgga gcacgcgcgt gccgcgcgcc cgcgccgcct cggccagcgg
     1141 cgccagccgg cgaattgggg agaccggcag gccggcggcc cggcgggacg gcttcatctc
     1201 cagcatcgac atcgaattct cgctcctatc cgtcttcgtt ggacgcccgg cgcgcccgac
     1261 cggcaagtat agcgagccgg ccgcttgtcg gctcggcgcg gactgcgtat cctagccggc
     1321 gagcgatggg caaacgaggg aggaagcgaa catgaccgac gttgtctctc ctctgggcgc
     1381 cgccggtacg gccgccgggc ggcgcgcggc gacgaccatc aagcaggagg cggtcgcctc
     1441 gcgcggcatg gtgaccagca accacccgct ggcctcgctg gccggcaccg agatgctgct
     1501 gatcggcggc aatgccgtcg acgccgccgt ggccaccctc ttcgccctct cggtggtcga
     1561 gccgatgatg gtcagcatct tcggcgccgg cttcctcacc atccggctgg ccgacggcac
     1621 catcaccacc atcgacgact acgccaccgt ccccggaggg gcgcggcccg acatgttcgt
     1681 gccggtggcc ggctcgctcg acaacgacgt cgtgggcggg ctcaacacca ccggctatct
     1741 ggcgccggcc acacccggca gcctgctcgg ctgggccacg gcggtcgagc gctacggccg
     1801 cctgccgctg gccgaggtgg tggccccggc ggtgcgctac gcccggcgcg gcttccgcgt
     1861 cagcccctac ctgcaccact tcatcgacct ctaccgcgac gatctggcgc gcttcccggc
     1921 caccgccgcc gtcttcctcc ccggcggaga cgttcccgcc atcgacgccg ccatccagcg
     1981 cgcagactac gccgataccc tggagcgggt cggcgcgcac ggccccggct ggctctacca
     2041 ggggccgctg ggcgacgcgg tcgcggccga catggcgcgc aacggtggcc tgatcacggg
     2101 cgaggatctg gccggctacc gcatctacga gcgcgccccg gtgcgcggca gctaccgcgg
     2161 ctacgagatc gtctcgatgg cgcccgcctc ctccggcggc acccacatca tccagatgct
     2221 gaacatcctg gagggcttcg acctggccgg catgggcttt ggctcccccc aggccgtcca
     2281 cctgatcgcc gaggcgatga agatcgcctt cgccgaccgc ttccgctaca tggccgaccc
     2341 cgagcgcgtc tccgtgccgg tcgactggct cacgtccaag agctacgccg ccgcgcgccg
     2401 cgccgagatc gagccggcgc gcgcccaggc ctacagcgcg gccctcgcgc ccgacggcga
     2461 gggcgcctcg acgactcact gctgcgccat ggacgccgag ggcaacgtcg tcaccaccac
     2521 gcagacgctc aacggcggct tcggctccaa ggtgacggtc cccggcaccg gcatgctgct
     2581 caacaactgc atgcacctga tggaccccac gccggggcgc accaactcga tcgcgccggg
     2641 caagcgcatc ctctcctcga tgtcgcccac catcgtgctg cgcgacggcc gcccgctgct
     2701 ggcgctgggc acgccgggcg gggtgcgcat cttcggcgcg gtgatgcagg cgatcatcaa
     2761 cgtcatcgac catggcatga cgctgcagca ggcggtcgag gcgccgcgcc tgtgggaccg
     2821 gggcccggtg ctggagctgg aggaaggctt ttccgagctg ccggcgctca aggccgacat
     2881 ggagcggcgc gggcacaccg tgcaggtggt gccgaaggtg gccggcggca tgaacggcat
     2941 cctgcgcgac gagcaggggc tgctgcacgg cgccgcctgc tggcgcgccg acggcgcccc
     3001 gatcggcttc tccggcggcg acgcccaggt tgacggcgcg gccggcttcg ccaccggcgt
     3061 gccgcgctag gtggaccagg ttgagaggaa aggcattgcc gacttgaccg atccgcgttc
     3121 gatcgtgcgc acgccgctgc tgctcccctc gtcgctggag caatactatg agaaaaccgg
     3181 acccgcccgg cgcgccgcgg acggcgacgt catcctgatc gccaccgggt gggatgcgcc
     3241 cgacggcaac atcgccgtcg tcctcggccc ggcggcctcg gagcgcgtct tcgcgctggc
     3301 ggacgagttc ttcggcggtg agccctactc cgtcatcgtc gagtcggagt ccgcggcgcc
     3361 ggttgaggct tcgctgcggg ccgccggctg ggaattggat gaggatgagc cggcgttgtt
     3421 cctgcagcca gtccctgccc caccgccgcc gcccgccgac ctcgacattc ggctggtgac
     3481 gaccgaggcg gagctggccg atttcatgga agtctccggc acgggccagc gctgggtgcc
     3541 gtcgctggcg gcggcgcgcg accccggcgt ggcgctcctc gtcggctacg tccagggcaa
     3601 gccggtcgcc acggcgcgca tcaacctcct gggccgggtg ggcgagatca acggtgtcgg
     3661 caccctgccg gcctatcgcc gccgtggcta cggcaccgcc atgacctggg ccgcgatcgc
     3721 cgagggcgta cgccgcggct gcgacgcgat cacgctctcg gcgtccgaga tgggctattc
     3781 cgtttaccgc cgcatgggct tcgtgccggt ctgccgctac cggacctacc tgccgccggg
     3841 cggccaagct gaggagtgag tgctggtgag cgcgaccaac ccgacgaccg aggacacctc
     3901 gctgacacgc cgcctggcgg agctggccgc ggagcacccg gacaagccgg ccatcatctt
     3961 cgcgccgctc gctggcgacg agcgcgccgt cagttgggcc gagctggagc gcgagagcaa
     4021 ccagatcgcc cgtctactgg ccgaccgtgg cgtgcgcggc gactccaccg tagtcggcgc
     4081 cgtctggaac tcgccggagt tgatcctctt caccctggcc gcctggaagc tgggcgcgct
     4141 ggtgctgccg ctgcgcgccg tcctgccccc gcgcgagcgc gacgccatcc tggagctggc
     4201 tcggccggcg ctggtgctgg ccgagtggga gagcgagcgc tggccggtca tccggccgga
     4261 ggagctggcc gccgcttccg gctacccggc tgacccgctg ccggcggtgc cgcaccccgg
     4321 caaggcgatc ggctcgggcg gctcgaccgg gcggcccaag ctgatcctcg atcccgcctg
     4381 gaacccggtc acggcgcgcg acaaccgccg cctgggcacc cggccgggtc aggtgcaact
     4441 gctggccgcg ccgctctacc acaactcgcc ctggctgcag tcgttcaacg ggctggccga
     4501 cgaccacact ctggtggtga tggagaagtt cgacgccacc cgtgcggcag acctgatcga
     4561 gcgctaccgc gtctcctacg cctacctgcc gccgatcctg atgcggcgcc tggcgctctt
     4621 gcccgacttc tgggagcgcg acttctccag cttcgacgcc atgcattcct cggcggccgt
     4681 ctgccccgtc tggctcaagc aggtctggat cgaccggctg ggcgccgaga aggtctacga
     4741 ggtctacggc tcggccgagg gcatcggcgc cactatcatc cgcggcgacg agtggctcca
     4801 gcaccgcggc agcgtgggcc gctcgaccaa ctgcgagatc cgtatcctcg acgaggacgg
     4861 cagcccgctg ccgccgggcg aggtgggtga gatctacttc cgccgcgtcg acggcaccac
     4921 gcccagcttc cactacgagg gcgcgccgcc ggtgcgctcg acgcccgacg gtttcgccag
     4981 cctgggcgac ctcggctggc tggacgccga cggctacctc tacatcgccg accggcgggt
     5041 ggacatgatc gtctccggtg gcgccaacgt ctacccgccc gaggtggagg ccgcgctcag
     5101 cgagcacccc ggcctggccg acgtggcggt gatcggcgtg ccggacgagg agtggggccg
     5161 gcgggtgcac gcggtggtgc agccgcacga cccggttaac ccgcccagcg tggccgagct
     5221 gaacgcgcac acgcgcgagc gcatcgccgc ctggaaggcg cccaagacct acgagctggt
     5281 gcccgagctg ccgcgcaacg aggccggcaa gatccgccgc gccgacctgg ccaccgcgcg
     5341 ccagaacggc tggatcgagg gcatgctgcg cgtgcgggag tgaggacggg cgagccctca
     5401 cctccaacgg ggttccctca tcccccagct ccttctcccc tgcgtgggcg aaggggagca
     5461 aatcatgact acggcttcgt gctccctctc ccgcgcacgg gagagggcgg ggtgagggct
     5521 tcccgtgagg gctaccccac tc
//
LOCUS       DASUBI010000554         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_887500, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000554 DASUBI010000000
VERSION     DASUBI010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887500"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFU72_10655"
     CDS             complement(<1..504)
                     /locus_tag="VFU72_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5423991.1"
                     /translation="MGGYTRREQLIILALALGTFTGVLNVYLLTPFLKLVAGAFGISQ
                     AAAGQLATVYALVAGLTGLLTAPLMDRYQRRLLLQFGLLIVALGTVLSALAWSFPLML
                     AARALAGFGAAFAGGCLYAAASDAFPDLGKRNRCVGILLSATGIAAVVGIPILTQLEA
                     LTSWRWAA"
     gene            complement(544..753)
                     /locus_tag="VFU72_10660"
     CDS             complement(544..753)
                     /locus_tag="VFU72_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423992.1"
                     /translation="MFDQIDLNLRYRAYAEQIERANCLPVHLPLEPVVLGSPRLAAAN
                     LLRRISARLDPEQARRAERQGRATV"
     gene            complement(1279..>2373)
                     /locus_tag="VFU72_10665"
     CDS             complement(1279..>2373)
                     /locus_tag="VFU72_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5423993.1"
                     /translation="SIFDLMLLVLPYQTDHLDERHRLAQIAERALARLDAVSGTAPAT
                     DMGRLPALIIEGNWERARQAAKGAYANNHVAWRHYALWVLATLAKNQGEADAASARIR
                     ELLPEGPETVPGNCFFPCGTNVQRIAAEMALGASDLPSARAWLEAHDRWLAWSGAALG
                     RAEGALGWAAYQHASGDAALARRHAEQALALASEPRQPLALIAVQRFLGQLDTEARHF
                     PDAEEHLRQSRELAEACAAPFERALTLLTLAGLRANQGKTDDARTLLAEVRAICEPLA
                     ARPTLERVAALEARLATAPKPAYPAGLTAREIEVLGLVAQGLTDAEVAERLFLSPRTV
                     SQHLRSVYNKLGVSSRAAATRFAVMHGLVE"
BASE COUNT          365 a          817 c          841 g          350 t
ORIGIN      
        1 cgccgcccag cgccaggagg tgagcgcctc gagctgggtc aggatcggga tgccgaccac
       61 ggcggcgatg ccggtcgccg agagcaggat accgacgcag cgattgcgct tgccgaggtc
      121 ggggaaggcg tcgctggcgg cggcgtagag gcagccgcca gcgaaggcgg cgccgaagcc
      181 ggccagggcg cgcgccgcca gcatgagcgg gaaggaccag gccagcgcgg agaggaccgt
      241 gccgagggcg acgatcagca gaccgaactg caggagcagt ctccgctggt agcgatccat
      301 cagcggcgcc gtcagcagcc cggtcagccc ggcgaccagg gcatagacgg tcgccagctg
      361 cccggccgcc gcctgcgaga tgccgaacgc accggcgacg agcttcagga agggtgtcag
      421 caggtagaca ttgagcaccc cggtgaaggt gccgagcgcg agcgccagga tgatcagttg
      481 ctcgcgccgt gtgtagccgc ccatgttccc cactccttcc gatcgcgccg ctccgcgcgt
      541 acctcagacc gtcgcacgcc cttgccgctc cgcgcgccgc gcctgttccg gatccagccg
      601 ggcggagatt cgccgcagca ggttggcggc ggccaggcgt gggctgccaa gcacgaccgg
      661 ctcaagcgga aggtgcaccg gcaagcagtt ggcgcgctcg atctgctcgg cgtaggcgcg
      721 gtagcgcaga ttcaggtcta tctggtcgaa catggtgtgc tcccttcagc caaccgggcg
      781 ttctgccacg gtcaaacgaa cagcacagcg gcggagcatg gtggcgagcg ccaggcgcgt
      841 ggccgccacg gggctgctgg gacgaggggg gcgcgggctc tggcggccgc gcgcctctcc
      901 gatctcgtgc aggtgccgct ggaggactct caactccatc tcggtgcgtg cgtcggccat
      961 gatcgcctcc cgtccattcg ccctcaaaac cccgtctctc gacattctca gtctcctgcc
     1021 gaacggcagc ccggaccacg aagaagcacg caattgcccc acgcgtttgc cccacgggtt
     1081 tgcgccccat ctcggaggct cccggcgaca tcgacgtgag tcgtgttcgc ggctcgtgcg
     1141 gttgccgccg ccgttctcat cgatctgctc gcaggatagg aagcgggact cgatcgcaca
     1201 tcgaggggat tgctcaatcg cggtggacaa gcacgcgaag gatggctacg tagcgttacg
     1261 tagccggagg ggaagaagtt actcgacgag gccgtgcatg acggcgaagc gggtggcggc
     1321 ggcgcgggag ctgacgccga gcttgttgta gacgctgcgc aggtgctggc tgacggtgcg
     1381 cgggctgagg aagagccgct ccgccacctc ggcgtcggtc aggccctggg ccactagccc
     1441 cagcacctcg atctcgcgcg ccgtcagccc cgccggatag gcgggcttcg gcgcggtcgc
     1501 gagccgcgct tccagcgctg ccacccgctc cagcgtcggc cgcgccgcca atggctcgca
     1561 gatcgcgcgc acctcggcca gcagcgttcg tgcgtcgtcc gtcttgccct ggttcgcccg
     1621 cagcccggcc agcgtgagca gcgtcagggc gcgctcaaag ggcgcggcgc acgcctccgc
     1681 caactcacgc gactgccgca ggtgctcctc ggcgtcggga aagtggcgcg cctccgtgtc
     1741 gagctggccg aggaagcgct ggacggcgat caaggccagc ggctggcgcg gctccgaggc
     1801 gagcgccagc gcctgctcgg cgtgccggcg tgcgagcgcg gcgtcgccgc tggcgtgctg
     1861 gtatgcggcc cagccgaggg cgccttcggc ccggccgaga gcggcgccgg accacgctag
     1921 ccagcggtcg tgcgcctcca gccacgcgcg cgcactgggg aggtcgctcg cgcccagagc
     1981 catctcggcg gcaatgcgct gtacgttggt gccgcacggg aagaagcagt ttcccggaac
     2041 ggtttcgggc ccctccggca gcagctcgcg tattcgggcg ctcgcggcgt cggcctctcc
     2101 ctgattcttc gccagggttg ccagcaccca cagcgcatag tgcctccaag cgacgtgatt
     2161 gttcgcgtag gcgcccttgg ccgcctgtcg agcccgctcc caattgccct caatgatcaa
     2221 ggcgggcagt cgccccatgt ccgtggcggg cgcggttcca ctcaccgcat caagtcgtgc
     2281 caaagcccgt tcagcgatct gcgcgagccg gtgtcgctca tccagatggt cggtctgata
     2341 tgggagcacg agcagcatga gatcgaatat aga
//
LOCUS       DASUBI010000555         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1186930, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000555 DASUBI010000000
VERSION     DASUBI010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186930"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..291
                     /locus_tag="VFU72_10670"
     CDS             64..291
                     /locus_tag="VFU72_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5423994.1"
                     /translation="MTERRRAPKPDSLQRPAPPDATPDPNYPAPSTADIVEIASVDSF
                     PASDPPSWIPISLSPCGPAEPAREEAEKEPS"
     gene            291..986
                     /locus_tag="VFU72_10675"
     CDS             291..986
                     /locus_tag="VFU72_10675"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEU5423995.1"
                     /translation="MVTLQLRIQRYNPEVDSKPHYEEYTVEADPADRLLDALNQIKWH
                     QDGTLTFRRSCAHGVCGSDAMRINGRNRLACKTLVQDVGERVTIEPLQAFPVIKDLVV
                     DMEPFYGGIRAMMPYLVNDDPPPATERLQSPAERERFDDTTKCIQCGACTSACPIWWT
                     SEYIGPAAIVNAHRFLFDSRDQAQEQRLAILGQHDGVWRCRTIFNCTEACPRGIQVEN
                     AIGQVKRELLWNR"
     gene            1091..2662
                     /locus_tag="VFU72_10680"
     CDS             1091..2662
                     /locus_tag="VFU72_10680"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5423996.1"
                     /translation="MTSTTEQITRPRMQAWPAAAAGALRAAFGVVWAINAYLTWRSSF
                     ADHYVGYLQNASQNQPSWLQPWFTFWINTVTAQPDLFIWATRIIETLLAIGLIFGIGR
                     KVVYLSGALFSLLIWSTAEGFSGPYTAGATNLGPALVYVLLFVALMVIDRDEGRSPYS
                     VDYYLERRWPGWRRVSELAPTPTLRHTPVRLSWRAQFLGALGIVVALVFLFGTLASAM
                     NVAAPTPGNAALAVSPLSLTTGEPVQNAYNPVLPPLLGTGDSVDVNLVATDTNVEIAS
                     GVSYQAWTFNGTVPAPIIHVRQGQTVNVTMTNHGTMKHSIDFHAAQVPANVDYRDVDP
                     GQSIQFSFKAEVPGVFMYHCGTPPALLHIGNGMFGAIVVDPAQGLPPADVSYVLVQNE
                     WYTRQVSGSTMAGDYAKMLADTPDEVTFNGVANQYKDHPLTAKAGQRIRIYLVDAGPN
                     LATAFHVIGGIFTAIYPDGDPSHALNGVQTYQVVPGQGVVFDIIIPNPGKYTFVDHSM
                     RNAALGAAGTIEVSP"
     gene            complement(2744..>4053)
                     /locus_tag="VFU72_10685"
     CDS             complement(2744..>4053)
                     /locus_tag="VFU72_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5423997.1"
                     /translation="LSLPPLVGRERELATLERHLTGAHGERPVLILAGEPGIGKTRLL
                     REAARRGLSAGLAVLISGGVRDPREPYAALRHLLRGYLREQDAANLHAWLDGCDWLAT
                     LLPELAHGEPRALAVDRLPLDGQRDYVEAALARFLSNIAGSAGTLLILDDLHLVDAAS
                     LDLLTALARSPRERPARILAAYRDTELAPGSPLDRWLSDQAAAGAASCRTLQPLDAAG
                     ARALLLALLAGGEPEPAEAIDRVTELSAGVPFYLVSYAEWLRAGRPEPVPWEIVRAVR
                     GRLAGLPQAGQDMLRRAAVAPLALRDRPVAVLEACCRAGLLRLNQDGAYVFTRDVIRQ
                     AILADLSSGERATLSRCTASETGTPMLDGDAGQSDAELAARYLEQAGDRASDLGAWQQ
                     AVTAYRDAIERLERTGPSPEAARLRAKLDRRRQERPASIADGS"
BASE COUNT          656 a         1501 c         1291 g          605 t
ORIGIN      
        1 tagccggcag ggggcgcgct gcccctcgat cacgcgcagc cgtttgacgc gagaggcagg
       61 aagatgaccg agcgcagacg tgcccccaag cccgactcct tgcagcggcc ggcgccgccg
      121 gacgccactc cggacccgaa ctaccccgcg ccgagcacgg cggatatcgt cgagatcgcc
      181 tcggtcgatt ccttcccggc cagcgatcct ccctcctgga tcccgatctc gctctcgcca
      241 tgcggaccgg ccgagccggc gcgagaggag gcggagaaag agccaagcta gtggttacac
      301 tccaactgcg catccaacgc tacaaccccg aggtcgacag taagccgcac tacgaggagt
      361 acaccgtcga ggccgatccc gccgatcggc tgctcgacgc gctcaaccag atcaagtggc
      421 accaggacgg cacgcttacc ttccgccgct cctgcgccca cggcgtctgc ggctccgacg
      481 ctatgcgtat caacggccgc aaccgcctcg cctgcaagac gctggtgcaa gatgtcggcg
      541 agcgcgtcac catcgagccg ctgcaggcgt ttccggtcat caaagatctc gtggtcgata
      601 tggagccctt ctacgggggc atccgcgcca tgatgcccta tctggtcaac gacgatccgc
      661 cgccggcaac cgagcgcctg caatcccccg ccgagcgtga gcgcttcgac gacacgacca
      721 agtgcattca gtgcggcgcc tgcaccagcg cctgtccgat ctggtggacc tcggagtaca
      781 tcggcccggc cgccatcgtc aacgcccacc gctttctctt cgacagccgc gatcaggccc
      841 aggagcagcg gctggcgatc ctgggccagc acgacggcgt ctggcgctgc cgcacgatct
      901 tcaactgcac cgaggcctgc ccccgcggca tccaggtcga gaacgcgatc ggccaggtca
      961 agcgcgaact gctctggaac cggtaatacc cattccggca agaccggggg cgcggtttga
     1021 ccgcgccccg gtgacgttat agttgttggg tttcttcggc ccctgggtgt atcgggaagg
     1081 agctaggcgg atgacatcga ccaccgagca gatcacgcgg ccgcgcatgc aggcctggcc
     1141 cgccgcggcg gcgggcgcgc tgcgcgccgc gttcggcgtc gtctgggcga tcaacgccta
     1201 tctcacctgg cgctcatctt tcgcggatca ctatgtcggc tacctgcaga acgcctcgca
     1261 gaaccagcct tcctggctgc aaccgtggtt caccttctgg atcaacacgg tcaccgcgca
     1321 gccggatctc ttcatctggg ccacgcggat catcgagacg ctgctggcca tcggcctgat
     1381 cttcggcatc gggcgcaagg tggtctacct ctcgggcgcc ctcttcagcc tgctcatctg
     1441 gagcaccgcg gagggcttta gcgggccgta cacggccggc gcgaccaacc tcggtcccgc
     1501 gctggtctac gttctcctgt ttgtcgccct gatggtcatc gaccgcgacg agggccgcag
     1561 cccctacagc gtcgactact acctggagcg gcgctggccc ggctggcggc gcgtctcgga
     1621 gctggccccc acgccaaccc tgcgccatac gccggtgcgc ctgagttggc gggcgcagtt
     1681 cctgggggcg ctgggcatcg tcgtcgcgct ggtcttcctc ttcggcaccc tggccagcgc
     1741 gatgaatgtg gcggcgccca cgcccggcaa cgccgcgctc gcggtatcgc ccctctcgct
     1801 taccaccggt gagccggtgc agaatgccta caaccccgtg ctgccgcccc tgctggggac
     1861 cggcgacagc gtcgacgtca atttggtcgc gacggacacg aacgtcgaga tcgccagcgg
     1921 tgtcagctac caggcctgga ccttcaacgg caccgtgccg gcaccgatca tccacgtacg
     1981 tcagggccag acggtcaacg tgacgatgac caaccacggg acgatgaagc actcgatcga
     2041 cttccatgcc gcccaggtgc cggccaatgt cgactaccgc gacgtcgacc ccggccagtc
     2101 gatccagttc tccttcaagg ccgaggtgcc gggcgtcttc atgtaccact gcggcacccc
     2161 gcccgccctg ctccacatcg gcaacggtat gttcggcgcg atcgtggtcg acccggcgca
     2221 ggggctgccc ccggccgacg tgagctacgt gctggtgcag aacgagtggt acacccgtca
     2281 ggtctcgggc tcgaccatgg ccggcgacta cgccaagatg ctggccgaca cgccggatga
     2341 ggtgacgttc aacggcgtcg cgaaccagta caaggatcat ccgctcacgg ccaaggctgg
     2401 tcagcggatc cgcatctatc tggtcgacgc cggcccgaac ctggcgacgg ccttccacgt
     2461 catcggcggg atcttcaccg cgatctaccc ggatggtgat cccagtcacg cactgaacgg
     2521 cgtgcagacc tatcaggtgg ttcccgggca aggcgtggtc ttcgacatca tcatcccgaa
     2581 ccccggcaag tacacctttg tcgaccacag tatgcgcaac gcggcgctcg gcgccgccgg
     2641 cacgatagaa gtctcgccgt agcgcgctgg agaacccgtg actcatcagg agaggcagac
     2701 gtcgcgtctg cctcttctgc agacaatcct cgcaccaacc gggctaggaa ccgtcggcga
     2761 tggaggctgg acgttcctgg cgccggcggt ccagcttggc gcgtaggcgt gccgcctccg
     2821 gcgagggtcc ggtgcgctcc agccgttcaa tggcatcccg gtacgcggtc acggcttgct
     2881 gccacgctcc cagatccgac gcccgatcgc cggcctgctc cagatagcgc gccgccagct
     2941 cggcgtcact ctggccggcg tccccgtcga gcatcggcgt ccccgtctcc gacgccgtgc
     3001 agcgcgacag cgtcgcccgc tcccccgaac tcaggtcggc cagaatcgcc tggcggatca
     3061 cgtcgcgggt gaagacgtac gcgccgtcct ggttcaaacg tagcagccct gcccggcagc
     3121 acgcctccag cacggcgacc ggccgatctc gcagcgccag gggcgccacc gccgcgcgcc
     3181 gcagcatatc ctgtcctgcc tgcggcagcc ccgccagccg cccgcgcacg gcgcggacga
     3241 tctcccaggg caccggctct ggacgtccgg cacgcagcca ctcggcgtag ctcaccaggt
     3301 agaacggcac cccggccgac aactcggtca cacgatcgat cgcctccgcc ggctccggtt
     3361 cgcctccagc caacagcgcc agcagcagcg cacgcgcgcc cgccgcatcc agtggctgga
     3421 gcgtccggca cgaggcagca ccagcggcag cctgatcgga taaccagcga tcgagcggac
     3481 ttcccggggc cagctccgtg tcgcgatacg cggcgaggat ccgcgccggc cgctcccgcg
     3541 gcgaccgcgc cagcgcggtg agaaggtcga gcgacgcggc gtcgaccagg tgcaggtcgt
     3601 cgaggatcag gagcgtaccg gcggagccgg cgatgttgct gaggaagcgc gccagcgcgg
     3661 cctcgacgta gtcgcgctgg ccgtcgagcg gcagccggtc tacagcgagc gcgcgcggct
     3721 cgccgtgcgc cagctcgggg aggagcgtgg ccaaccagtc gcagccgtcg agccacgcgt
     3781 gcaggttggc cgcgtcctgc tcgcgcaggt agccgcgcag gagatggcgc agcgcggcgt
     3841 acggctcccg cgggtcgcgg accccaccac tgatcaagac cgccagcccg gcgctcagcc
     3901 cgcgccgcgc agcctcgcgc agcagccggg tcttaccgat ccccggctcg ccggcaagga
     3961 tcaacaccgg ccgctccccg tgcgcgcccg tcaggtgccg ctccagcgtc gccagctcgc
     4021 gctcgcgccc caccaggggc ggcagactca gac
//
LOCUS       DASUBI010000556         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_838380, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000556 DASUBI010000000
VERSION     DASUBI010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838380"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFU72_10690"
     CDS             <1..589
                     /locus_tag="VFU72_10690"
                     /inference="COORDINATES: protein motif:HMM:NF004813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5423998.1"
                     /translation="APPRELERLDLTRRLIALLDHDEAVVAGIGNSSFDLFGAGHRPQ
                     NFYMLGSMGMAVPIGLGLALAQPAREVLVLEGDGSLLMNLGGLATVAMVAPPNLTIVA
                     WDNGGYQMTGNQPTAAAEAADLVAMARAAGIARAAWACDEQHFEALVRAALDRPGPAF
                     IGARVRSQPSQSHPELDPVVLKYRFMLGIGARAAE"
     gene            616..2163
                     /locus_tag="VFU72_10695"
     CDS             616..2163
                     /locus_tag="VFU72_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5423999.1"
                     /translation="MEFSALHQALERWAAARPEKTFLREVEDGRRLSFAGCLAAVNGL
                     RQELGAEPLTIVLALPGGVAASVVWLAALCGGHRLIPCSPEITIDEAEALAARYQPDL
                     LVAETPEVTHRLDRVAPRSLTRADLEAGVDRWIREPRPTPGDWRDGELCLTTSGTTGT
                     PKGISLRAGQLAWTAEQIRQSHRLSERDRGLAVLPFFHINAPVVSLCSSLLAGAELVI
                     APRFSRSRFWSWIEHEEITWASIVPTIVAVLLQTERPAWLPGTLRFVRTASAPLPAVQ
                     LRAFERRFGIPVIETYGLSEAASTVSANPVPPGRHKPGSVGKPLGVALRVCLPRAGGA
                     EEHIELIDVPAGVEGEICISGPNVIAGYDGGASAAAFQGGWFRTGDLGYLDEDGYLFI
                     SGRLKDVINRGGEKIAPREVEELLLTHPAVLDAAVVGRADPIYGELPVAYVMLAPHAY
                     GMTTAPLADFCAQRLSPYKVPAEIVAVDSLPRTRTGKIQRHVLRSTLRAEPELTAPNA
                     AARYGVA"
     gene            2197..3492
                     /locus_tag="VFU72_10700"
     CDS             2197..3492
                     /locus_tag="VFU72_10700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5424000.1"
                     /translation="MSAATGKKPSRAYLHEIDMLRAVTALSVVAVHVTALSLFLNHSS
                     LGTDVQNAVVTALHFTREVFIAITALVLVYGYVGKPFPFRHFWRRRGLGVLLPYIAWS
                     LFYVAFSTPFHPVGHWLKTAGWDILMGSASFQLYYILLTIQFYLALPLILRWVRRLGR
                     NPWRLLLGSFVLQLVMMYVDYNYVETGHFASTHLGHQINEYHWRFLPFYQLYVVCGAL
                     AALYLDQLRAFIRRHGRLIVAGMLFGLALLWGRYAYALGVEHRGQDSATAVFQPVVVF
                     YSLTTILFLYWFTDRWALRRFGHGAPMSARSRFWQHLSDISFGIYLVHAFILTRVLGH
                     IAPRLPVAVPLALRVALLWGIVAGSSVALCLLFLYTPLLSRLIGRPCMLPARWLDWPA
                     RRLAALRRGRHRPAVAPAFEPARPEPAVREVARLDVGGE"
     gene            3673..4734
                     /locus_tag="VFU72_10705"
     CDS             3673..4734
                     /locus_tag="VFU72_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5424001.1"
                     /translation="MNRKLGWVPRALVIVVLLALSVFAQTAGVIQRAPLARQGRARAA
                     SAPSTRSRREGGIVSERRSKPGEGYLDPAMRSGAISARHPRIDCQPAPLSVYHSCVYH
                     DARGLAMTFYLFFPRHYDPQARYPLVLLLHGGGERADPANPAEANREKLLDDPYAQVW
                     GPGFQGPHSVEVQARWPSFVVAPQLVFPNRWVDVPPGDGSHRMAPEPTDSLRLAKEIV
                     DTLRAGYPAIDAHRLYITGMSLGGYGTWDAIQRWPHEFAAAAPICGAGDPARAAELTQ
                     LPLWAFHGADDPIVPVSGTRQMIEAIRAAGGHPRYTELPGVGHGSWVYAYALSGAPSP
                     TPDFFAWLFAQHLPSAPES"
BASE COUNT          642 a         1804 c         1605 g          769 t
ORIGIN      
        1 cgccccgccc cgcgagctcg agcggctgga cctgacccgg cgtctgatcg ctctgctgga
       61 tcacgatgag gccgtggtgg ccggcatcgg caatagcagc ttcgacctct tcggcgccgg
      121 gcaccgcccg cagaacttct acatgctcgg cagcatgggc atggccgtac ccatcggcct
      181 cgggctggcg ctggcgcagc ccgcgcgcga ggtgctggtg ctggagggcg acggctccct
      241 gctgatgaac ctcggcgggc tggcaaccgt cgccatggtc gccccgccca acctgaccat
      301 cgtcgcctgg gacaacggcg gctaccagat gaccggcaac cagccgaccg cggccgcgga
      361 ggcggccgat ctggtcgcca tggcccgggc cgccggcatc gcgcgcgccg cctgggcctg
      421 cgacgagcag cacttcgagg cgctggtgcg cgcggcgctc gaccggcccg gcccagcctt
      481 catcggcgcg cgcgtccgca gccagccgag ccagagccac ccggagctcg acccggtcgt
      541 gctgaagtac cgcttcatgc tcggtatcgg cgcgcgggct gccgagtagc gcgacgagcg
      601 agattgaagg agaccatgga attctcagct ttgcaccagg cgctcgagcg ctgggctgcc
      661 gcgcggccgg agaagacctt cctacgggag gttgaagacg gccgacggct cagcttcgcc
      721 gggtgcctgg cggccgtgaa cggcctgcgg caggagctcg gcgctgagcc gctgacgatc
      781 gtgctggcgc tccccggcgg cgtggccgcc tcggtcgtct ggctggccgc gctctgcggc
      841 ggccaccggc tgatcccctg ctcgccggag atcaccatcg acgaggccga ggcgctggcc
      901 gcgcgctacc agcccgacct gctggtggcc gagacgcccg aggtgacgca ccgcctcgac
      961 cgcgtcgctc cccgcagcct gacccgcgcc gacttggagg ccggcgtcga ccgctggata
     1021 cgggagccgc ggccgacgcc gggcgactgg cgcgacggcg agctctgcct gacgacctcc
     1081 ggtaccaccg gcacgcccaa ggggatcagc ctgcgcgccg gtcaactcgc ctggaccgcc
     1141 gagcagatcc gccagagcca ccggctcagc gagcgcgatc gtgggctggc cgtgctcccc
     1201 ttcttccata tcaacgcgcc ggtggtcagc ctctgcagct cgctgctggc gggcgccgaa
     1261 ctggtgatcg cgccgcgctt cagccgcagc cgcttctggt cctggatcga gcacgaggag
     1321 atcacctggg ccagtatcgt gccgacgatc gtggcggtgc tcctgcagac cgagcgcccc
     1381 gcctggctcc ccggcacgct gcgcttcgtg cgcaccgcct cggccccgct gccggcggtg
     1441 caactgcgcg ccttcgagcg ccgcttcggc atcccggtga tcgagaccta cggtctctcc
     1501 gaggccgcca gcaccgtgag cgccaacccg gtcccgcccg ggcggcacaa gcccggctcg
     1561 gtcggcaagc cgctcggcgt ggcgctgcgc gtctgcctcc cgcgcgccgg cggcgccgag
     1621 gaacacatcg agctgatcga cgtgccggcc ggcgtggagg gcgagatctg catcagcggc
     1681 cccaacgtca tcgccggcta cgacggcggc gccagcgcgg ccgccttcca gggcggttgg
     1741 ttccgcaccg gcgatctcgg ctatctcgac gaggacggct atctcttcat ctcgggacgg
     1801 ctcaaggacg tgatcaaccg cggcggcgag aagatcgcac cgcgcgaggt cgaggagcta
     1861 ctgctgaccc acccggcggt gctcgacgcc gcggtcgtcg gccgcgcgga cccgatctac
     1921 ggcgagctgc cggtggccta cgtcatgctg gcgccgcacg cctacggcat gacgaccgcg
     1981 ccgctggcgg acttctgcgc ccagcggctc agcccctaca aggtgccggc cgagatcgtg
     2041 gccgtcgact ccttgccacg aacgcgcacc ggcaagatcc agcgccacgt gctgcgctca
     2101 acgctccgcg cggagccgga gctgaccgcg cccaatgccg ccgcgcgcta cggcgtggcc
     2161 tagcgtgaag gagggtcagc gaagggggag aaccgggtga gcgcagccac ggggaaaaag
     2221 ccgtcacgag cgtatctgca cgagatcgac atgctgcggg cagtgaccgc gctgtcggtc
     2281 gtcgccgtgc acgtcacggc gctgtcgctc tttctgaacc attcaagcct gggaaccgac
     2341 gtgcagaacg cggtcgtgac cgcgctgcac ttcacccgcg aggtcttcat cgccatcacc
     2401 gcactggtgc tggtgtacgg ctacgtgggc aagcctttcc ccttccgcca cttctggcgc
     2461 cggcgcgggc tgggcgtgct cctcccctac atcgcctgga gcctgttcta cgtcgccttc
     2521 tcgacgccct ttcacccagt ggggcactgg ctgaagacgg ccggctggga catcctgatg
     2581 ggcagcgcct cattccagct ctattacatc ctgctgacca tccagttcta cctggcgctg
     2641 ccgctaatcc tgcgttgggt gcggcggctc ggccggaacc cctggcgcct gctgctcggc
     2701 agcttcgtcc tgcagctcgt catgatgtac gtcgactaca actatgtcga aaccgggcat
     2761 ttcgccagca cgcacctcgg ccaccagatc aacgagtacc actggcgctt cctccccttc
     2821 taccagctct acgtcgtctg cggcgcgctg gccgcgctct acctcgacca actgcgcgcc
     2881 ttcatccggc gtcacggccg gctgatcgtg gccggcatgc tcttcggact ggcgctgctc
     2941 tgggggcgtt acgcctacgc gctcggcgtg gagcaccgcg gccaggactc agccaccgcg
     3001 gtcttccagc cggtggtggt gttctacagc ctgacgacga tcctcttcct ctactggttc
     3061 accgaccgct gggcgctgcg gcgcttcggc cacggagcgc cgatgagcgc gcgctcgcgc
     3121 ttttggcagc acctctcgga tatctcgttc ggcatctatc tggtgcacgc cttcatcctg
     3181 acccgcgtgc tgggccatat cgcgccgcgg ctgccggtgg ccgtgccctt ggctctccgc
     3241 gtcgcgctgc tctggggcat cgtggcgggc agcagcgtcg cgctctgcct cctcttcctc
     3301 tacacgccgc tgctcagccg gttgatcggc cgcccctgca tgctcccggc gcgctggctc
     3361 gactggccgg cccggcggct ggccgcgttg cgccgtggtc gccatcgccc ggccgtggcg
     3421 ccagcctttg aaccagcccg gcccgagccg gccgtgcgcg aggtcgcgcg gctggatgtc
     3481 ggcggggagt agggcgcacc cggtcttctc ccatcgcgtg tagcctgtag gggcgggttt
     3541 cgaacccgcc cttctctagt gcgcgactca cgttttcatt gacgagcacc cattgccgct
     3601 tgcaacgtgg cataatctcg ccaccgtaca tgcaagggtt cgcgggcgtc ggagcgcgcg
     3661 ggtggggcaa aattgaacag gaaattgggc tgggttccgc gcgcgctcgt catcgtcgtg
     3721 ctgctcgcgc tgtcggtctt cgcgcagacc gcgggcgtga tccagcgcgc cccgctggca
     3781 cgccaggggc gggcgcgggc cgcatcggcg ccgagcacgc gctcgcggcg cgaggggggt
     3841 atcgtgtccg agcgacggtc aaagcccggc gagggctatc tcgacccggc catgcgctcc
     3901 ggcgcgatct ccgcgcgaca cccgcgcatc gactgccagc ccgcgccgct cagcgtctac
     3961 cactcgtgcg tctaccacga cgcacgcggc ctggccatga ccttctatct gttcttcccg
     4021 cgtcactatg acccacaggc gcgctacccg ctggtcttgc tgctgcacgg cggcggggag
     4081 cgagccgatc cggccaatcc tgccgaggcg aaccgcgaaa aactgctcga cgatccctac
     4141 gcccaggtct ggggaccggg cttccagggt ccgcacagtg tcgaggtcca ggcgcgctgg
     4201 cccagcttcg tggtcgcgcc gcagctcgtc ttcccgaacc gctgggtcga cgtgccgccc
     4261 ggcgacggct cccaccgcat ggcgccggag ccgaccgact cgctgcgcct ggccaaggag
     4321 atcgtcgaca cgctgcgcgc cggctatccg gcgatcgacg cccaccgcct ctacatcacc
     4381 ggcatgtcac tcggcggcta cggcacctgg gacgccatcc agcgttggcc gcacgagttc
     4441 gccgcggccg ccccaatctg cggcgcgggc gacccggcgc gcgccgccga actgacccag
     4501 ctgccgctct gggccttcca cggcgccgac gacccgatcg tgccagtcag cggcacccgc
     4561 cagatgatcg aggctattcg cgccgctggc ggccacccgc gctacactga gctgcccggc
     4621 gtcggtcacg gctcctgggt ctacgcctac gcgctgtcgg gcgctccctc gccgaccccc
     4681 gacttcttcg cctggctctt cgcccagcac ctgccgtccg cgccggagtc gtaggccagg
     4741 cagcaatcct cccggcgacg atgtgcacgg gctcgccaag agcggctgtt ccctgcgcct
     4801 gaggcgctct ggtacccagg
//
LOCUS       DASUBI010000557         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_52008, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000557 DASUBI010000000
VERSION     DASUBI010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52008"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFU72_10710"
     CDS             <1..998
                     /locus_tag="VFU72_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424002.1"
                     /translation="LRPKTGLKDMFIELNPGSKSAPMAKAGWTIPVRNTLPDINPDEV
                     YSLLDADTRDYLRLLISGAGEGLHKRGGDLQEVFRRFEPTHKDLALVNKYVALRHQNL
                     RRLIHQLNVLNGALAQKGDDLAQLVQTSSVVFHSLASQDANITRAVGDLPGTLRQTTQ
                     TFGKVKQLADVLGPAVTNLRPAAKALNIANQHITPLAKEAAPILRTEIRPFVRDARPV
                     VRSLKPASQNLAAATPNLTSTFTVLNHLFNMIGYNPNGKEGPGVAKRDEGFLFWLAWL
                     DHNGTALFSSSDANGPFRPITLGAPCSTLKQTGDANPPLGMILAPALSDPRICGS"
     gene            998..2473
                     /locus_tag="VFU72_10715"
     CDS             998..2473
                     /locus_tag="VFU72_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5424003.1"
                     /translation="MVKQAPTLGRLLTMVVFALSCFGLLLFLWLSFGGPIPLKPQGYR
                     VHVAFPEATQLGLEADVRVAGVSVGKVVKKDIDPAHPNRTVATLELNNRFAPLATSAR
                     AILRQKTLLGETYVELTPGDPKGPKIKENGWLPDARVKKTVELDEVFKALDPVTRKAF
                     QNWQQSLAKSIGSNANQRGQDLNDAFGNLPGFAQDADGVLTVLDEQSAEVRNLVKNTG
                     VVFGALNQNQAQLHNLITNSSDVFSATQKEKEALAATFRIFPTFLDESKATFAKLEGF
                     SKNTDPLIRQLQPAIKDLRPTLVNTRALSPDLQHLFRNLDPLIKASKTGLPALRDVLD
                     GTGPVLQQLQPFLENLNPVLQWLEYNQRLVGDFISNGAGALVDTVPVRDPNIEIGHYL
                     RQFGPMGPESVAIYPNRPTENRGNAYLPPTAYSGPERAQKMIVPSFDCKNTASGGPET
                     TQFPDKSGDPSCWVAPPPSYPPGNTSQFPHITALDYSKPSR"
BASE COUNT          455 a          933 c          819 g          358 t
ORIGIN      
        1 tgctgcggcc gaagaccggc ctgaaggaca tgttcatcga gctcaacccg ggctcgaaga
       61 gcgcgccgat ggcgaaggcc ggctggacga tcccggtgcg caacacgctg ccggacatca
      121 accccgacga ggtctactcg ctgctggacg ccgacacgcg cgactacctg cggctgctga
      181 tctccggtgc cggcgagggc ctgcacaagc gcggcggtga cctgcaggag gtcttccgcc
      241 gcttcgagcc gacgcacaag gacctcgcgc tcgtcaacaa gtacgtcgcg ctgcgccacc
      301 agaacctgcg ccgcctgatc caccagctca acgtgctcaa cggcgcgctg gcgcagaagg
      361 gcgacgacct cgcgcagctc gtgcagacgt cgtcggtcgt cttccactcg ctcgcctccc
      421 aggacgcgaa catcacgcgc gccgtcggcg acctgcccgg cacgctgcgc cagacgactc
      481 agacgttcgg caaggtcaag cagctggccg acgtcctcgg ccccgcggtc acgaacctgc
      541 gcccggcggc caaggcgctg aacatcgcca accagcacat cacgccgctg gccaaggagg
      601 ccgcgccgat cctgcgcacg gagatccggc cgttcgtgcg cgacgcgcgc ccggtcgtcc
      661 gcagcctgaa gcccgcctcg cagaacctgg cggcggcgac gcccaacctc acgagcacgt
      721 tcaccgtgct caaccacctg ttcaacatga tcggctacaa cccgaacggc aaggagggcc
      781 ccggcgtcgc caagcgcgac gagggcttcc tgttctggct cgcgtggctg gaccacaacg
      841 gcacggcgct gttctcgagc tctgacgcga acggcccgtt ccgcccgatc acgctcggcg
      901 ccccgtgctc gacgctcaag cagaccggcg acgccaaccc gccgctgggc atgatcctgg
      961 cgccggccct ctccgaccca aggatctgtg gttcgtagtg gtcaagcagg cgccgacgct
     1021 cggaaggctg ctcacgatgg tggtgttcgc gctgtcgtgc ttcggcctgc tgctgttcct
     1081 ctggctgagc ttcggcggcc cgatcccgct caagccgcag ggctatcgcg tccacgtcgc
     1141 gttcccggag gccacgcagc tcggcctcga agcggacgtg cgcgtcgcgg gcgtgtcggt
     1201 gggcaaggtc gtcaagaagg acatcgaccc ggcgcacccg aaccgcacgg tcgcgacgct
     1261 cgagctgaac aaccgcttcg cgccgctggc cacgagcgcc cgggcgatcc tgcgccagaa
     1321 gacgctgctg ggcgagacct acgtggagct gacgccgggc gatccgaagg ggccgaagat
     1381 caaggagaac ggctggctgc cggacgcgcg cgtgaagaag accgtcgagc tcgacgaggt
     1441 cttcaaggcg ctggacccgg tgacgcgcaa ggcgttccag aactggcagc agtcgctcgc
     1501 caagagcatc ggatcgaacg ccaatcagcg cggccaggac ctcaacgacg cgttcggcaa
     1561 cctgcccggg ttcgcccagg acgccgacgg cgtgctcacc gtgctcgacg agcagagcgc
     1621 cgaggtccgc aacctcgtca agaacaccgg cgtcgtgttc ggcgcgctca accagaatca
     1681 ggcgcagctg cacaacctga tcacgaactc gtcggacgtc ttctccgcca cgcagaagga
     1741 gaaggaggcg ctggccgcga ccttccggat cttcccgacg ttcctggacg agtccaaggc
     1801 gacgttcgcc aagctcgagg gcttctcgaa gaacaccgac ccgctgatcc gccagctcca
     1861 gccggcgatc aaggatctgc gtccgacgct cgtcaacacg cgtgcgctgt cgccggacct
     1921 gcagcatctg ttccgcaacc tcgatccgct gatcaaggcg tcgaagaccg gcctgccggc
     1981 gctgcgcgac gtgctcgacg gcaccggccc ggtgctccag cagctgcagc ctttcctgga
     2041 gaacctcaac ccggtcctgc agtggctcga gtacaaccag cggctggtcg gggacttcat
     2101 ctccaacggc gccggcgcgc tggtcgacac cgttccggtg cgcgacccga acatcgagat
     2161 cggtcactac ctgcgccagt tcggcccgat gggcccggag agcgtggcga tctacccgaa
     2221 ccggccgacc gagaaccgtg gcaacgccta cctgccgccg acggcctaca gcggcccgga
     2281 gcgcgcgcag aagatgatcg tgccgagctt cgactgcaag aacacggcca gcggcgggcc
     2341 ggagaccacg cagttccccg acaagagcgg cgacccgtcg tgctgggtcg cgccgccgcc
     2401 gtcctacccg cccggaaaca ccagccagtt cccgcacatc acggcgctgg actactccaa
     2461 gccgagccgt tgagaatcag gggtctgtcc cctcagtctc aaatgagaat gaggggactg
     2521 tcccctcatt ctcacgcgac ctgggcgtcg cgggcggcgg ccacg
//
LOCUS       DASUBI010000558         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1163913, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000558 DASUBI010000000
VERSION     DASUBI010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163913"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VFU72_10720"
     CDS             <1..1364
                     /locus_tag="VFU72_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424004.1"
                     /translation="VAHSIGTFAWTYDGNRLLYVSADDGAVHSFDITSGADRTLSTRT
                     ADPCAGPFFTTTRGDQLLFLDEASGGTTLVALNVDTAQERVYPNLPVGFPASVLPAAY
                     VGADVTGSFVDLVQAYPTRITRLDLASGTLTTLLSDEWRGVLTFSPDHQQVVLVDNPY
                     GKPTVASIRDLRSGQEVSFQYVGWLTFEPSAITPSSFLAWSRTWAREDRPVAAGAVAR
                     SWTWGPSPFAMEEEQYADAPGGRRPVLYYDKSRMEINTPYGDQSSRWFVTNGLLAKEL
                     ITGQMQVGNDPSAVVNRGPAQVNVAGDSNDPNGPTYASLTHLLAAPPVAVGSEIKASL
                     GRSGAVGNAGPGGVHATQLVSESNHTIADVFWAYLTSQGPIWDGARITSGQLFDPTFF
                     ATGFPITEAYWTRVKVGGVERDVLLQCFERRCLTYTPSNPDGWKVEMGNVGRHYYAWR
                     YGG"
     gene            1481..2395
                     /locus_tag="VFU72_10725"
     CDS             1481..2395
                     /locus_tag="VFU72_10725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5424005.1"
                     /translation="MEHLAVVIVNHNTSELLLECLARLTNSRTTVPYTIYVVDNGSTD
                     DSLARLAERFPEVVAIAAGRNLGFAGGNNLALRRALADVPPGAARAARAVLLLNSDCF
                     VEPETIARLARFLDEHPEAGVVGPRLTLPDGRLDLACRRSFPTPASAFWKLTGLARLF
                     PRSPRFARYNLTYLDPEQVAAVDSVVGACMLVRLGAIDAAGLLDEAFFMYGEDLDWAY
                     RIKEQGWQVYYNPAARALHFKGATSSKRSYRLIIAFYHAMYLFHRKHYAARTFVLVNW
                     LIALGIVARGGFALLLNLLRPRAAKRVA"
     gene            2392..3183
                     /locus_tag="VFU72_10730"
     CDS             2392..3183
                     /locus_tag="VFU72_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEU5424006.1"
                     /translation="MTALPHDAPDPGAGAGGGGGSGRERLAARVAPADHLHLIPPAGA
                     GVGAEVALAEPEPGPLLAGCPYHTAGYRQALKRLLDLILSLLVLLAAAPLMLLLALAI
                     KLDSPGPVFYTQERVGLGGRCFRLLKFRSMTVDAEAATGPVFATRDDPRTTTVGRVMR
                     RLSLDELPQFINVLRGEMSVVGPRPERPHFVGQFSQAIPGYAERHAMKAGITGWAQVN
                     GLRGATSIEERTGYDLAYIAHWSLRLDLKIVLLTARQIVRGENAY"
     gene            complement(3441..4730)
                     /locus_tag="VFU72_10735"
     CDS             complement(3441..4730)
                     /locus_tag="VFU72_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424007.1"
                     /translation="MATREQHGPVVSWLIGLVGREQWAAVLFALRINIFSLALLGFWT
                     PLNNLILPDQVSQLVPDSLKGSALGVITFLGIGIATVVQPIAGRIDDRLALPHRRKTF
                     IAGGAAAGLLGVLLFSLAPSFLWLLVAYIVMQLAVNVAQAAFQALIPDLVKPRDRGLA
                     SGIKDGLGVVGAALGVVGVGLLVNLLWGKYLALAYLALLLGGLAYLNLRWVPDVPPPP
                     DDERIHSLRDIQLGAMFRSFGAAFKTNRVFRLAVIARFFFLLGLYPVERLFLYYLQNR
                     FNITSLSQVSLFLVAAIVLAALSAWVAGALSDRLGRTPVLRIMAVLGTIGVAGVAFSP
                     WLWLVGIFGACVASAFGGFQSVNWAQMADVIPEGRSAEYYGLSNIATAGSGAVAGLFG
                     PLADFLNALLPGGTYFITFGLAALIALSSLIPLRRLP"
     gene            complement(4734..>5645)
                     /locus_tag="VFU72_10740"
     CDS             complement(4734..>5645)
                     /locus_tag="VFU72_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5424008.1"
                     /translation="GAAALSLLFGLNWRWFVLPFGLAMVLLLLFGSYRAISRVLEFIL
                     LVFVAYIVSAFLAHPDWGQVLAHTLIPHIRFQHAYIEGMLAMLGTTLTSYAYVWESIE
                     ESEERLPIRELGLARADAGFGTFFAVAIFWFILISTGATLGVSHQPVTTAAQAARALE
                     PAAGALASDIFAVGLLASAVLAVPVLSATTAYMLGEEFGWRRGLSEPVRRARRFYIAV
                     GGAILFAVAVAFAGVSPIRLLYISSIAGGIGTPISLAFLLLVARDTRVMQSHPIGPVL
                     TGIGWAATIMIGAISLYFLGQPLLGLV"
BASE COUNT          928 a         1981 c         1942 g          794 t
ORIGIN      
        1 aggtcgccca ctcgatcggc accttcgcct ggacctacga cggcaatcgc ctcctctacg
       61 tctcggccga cgacggcgcc gtgcacagct tcgacatcac cagcggcgcc gatcgcaccc
      121 tctcgacgcg caccgccgac ccctgcgccg ggccgttctt caccaccacc cgcggcgatc
      181 agctcctctt cctcgacgag gcatccggcg gcaccacgct ggtggcgctg aacgtcgaca
      241 ccgcccagga gcgcgtctac cccaatctgc cggtcggctt ccccgccagc gtgctgcccg
      301 ctgcctacgt cggcgccgac gtcacgggga gcttcgtcga tctggtgcag gcctacccga
      361 cgcgcatcac ccggctcgat ctcgccagtg gcacgctgac caccctcctc tccgacgagt
      421 ggcgtggcgt gctcaccttc tcgcccgatc accagcaggt cgtgctggtc gacaacccct
      481 acggcaagcc gacggtggcc tcgatccgtg acctgcggag cggccaggag gtgtctttcc
      541 agtacgtcgg ctggctgacc ttcgagccct ccgcgatcac gccgtcgtcg ttcctggcct
      601 ggagtcgcac ctgggcacgc gaggatcgcc cggtggcggc cggcgcggtg gcgcgcagct
      661 ggacctgggg gccgagcccc ttcgccatgg aagaagagca atacgccgac gcgcccggcg
      721 gccggcgtcc ggtgctctac tacgacaaga gccgcatgga gatcaacacg ccctacggcg
      781 atcagagctc gcgctggttc gtcaccaacg gcctgctggc caaggagctg atcaccggcc
      841 agatgcaggt cggcaacgac cccagcgccg tggtcaaccg cggcccggcc caggtcaacg
      901 tggccggcga ctccaacgac cccaatggac ccacctacgc ttcgctgacg caccttctcg
      961 cggcgccgcc ggtcgccgtc ggcagcgaga tcaaggcatc gctcggccgg agcggggcgg
     1021 tggggaacgc cgggccgggc ggcgtccacg ccacccagct cgtctccgag tccaaccaca
     1081 ccatcgccga cgtcttctgg gcctacctca ccagtcaggg tccgatttgg gacggcgcgc
     1141 gtattaccag cggacagctc ttcgatccga ccttcttcgc caccggcttc ccgatcaccg
     1201 aagcctactg gacgcgagtg aaggtcggcg gtgtggaacg cgacgtgctg ctgcaatgct
     1261 tcgagcggcg gtgcctgacc tacacgccgt ccaatcccga cggctggaaa gtcgagatgg
     1321 gaaacgtcgg gcgccactat tacgcctggc gctacggagg gtagtggaga cggacacgca
     1381 aggtcaggtt gagctgccgg caattcctgg gtgcgcgggc atcgtggcgg caggcgatgc
     1441 ttgcgcgtct gccgctctgc gctccatact aggccacgct atggagcacc tggccgttgt
     1501 catcgtcaat cacaatacct ccgagctcct cctggagtgt ctcgcccggc tgacgaacag
     1561 ccgcaccacc gtcccctaca ctatctacgt ggtcgacaac ggctcgaccg acgacagcct
     1621 ggcgcgcctg gccgagcgct ttcccgaggt cgtggccatc gcggccgggc gcaatctggg
     1681 cttcgcgggc ggcaacaacc tggccctgcg ccgcgcgctg gccgacgtgc cgcccggcgc
     1741 cgcgcgggcg gcccgcgccg tgctcctgct caactccgac tgcttcgtcg agccggagac
     1801 catcgcgcga ctggcgcgct tcctcgacga gcacccggaa gcgggcgtgg tcgggccgcg
     1861 gctgactctc cccgatggac ggctggacct cgcctgccgt cgctccttcc cgaccccggc
     1921 cagcgccttc tggaagctga ccggcctggc gcggctcttc ccgcggagcc cgcgcttcgc
     1981 gcgctacaac ctgacctatc tcgatccgga gcaggtcgcg gcggtcgact cggtggtcgg
     2041 cgcctgcatg ctggtgcgtc tgggcgccat cgatgcagcc ggtctcctcg atgaagcgtt
     2101 cttcatgtat ggcgaagacc tggattgggc ctatcgcatc aaggaacagg gctggcaggt
     2161 gtactacaac ccggcggcgc gagcgctgca tttcaaggga gcgaccagtt cgaagcgcag
     2221 ctaccgcctc atcatcgcct tctaccacgc gatgtacctc ttccaccgca agcactacgc
     2281 ggcgcgcacc ttcgtcctcg tgaactggct gatcgcgttg ggcatcgtcg cgcgcggtgg
     2341 cttcgccttg ctgctgaacc tgctgcgccc gcgcgcggcg aagcgggtcg cgtgaccgcg
     2401 ctcccgcacg acgcgcccga tcctggggcc ggggctgggg gtgggggtgg gagtgggcgc
     2461 gagcggctgg cggcgcgcgt cgcacccgcc gaccacctgc acctgattcc gccagcgggg
     2521 gcgggtgtcg gggcggaggt ggccctggcc gagccggagc ccgggccgct gctggccggc
     2581 tgcccctatc acacggcggg ctaccgccag gcgctgaagc ggctgctcga cctgattctg
     2641 agcctgctgg tcttgctcgc cgctgctccg ctgatgctcc tgctggcact ggcgatcaag
     2701 ctcgactcgc ccggtccggt cttctacacg caggagcgcg tcgggctggg cggacgatgc
     2761 ttccgcctgc tcaagtttcg ctcgatgacg gtcgacgccg aggcggcaac cggcccggtc
     2821 ttcgccaccc gcgacgaccc gcgcacgacc acggtcggcc gcgtcatgcg ccgtctctcg
     2881 ctcgacgagc tgccgcagtt catcaacgtg ctgcgcggcg agatgagcgt cgtcggcccg
     2941 cggccggagc gtccccactt cgtgggtcag ttcagccagg cgatccccgg ctacgccgag
     3001 cgccacgcca tgaaggcggg catcaccggc tgggcgcagg tcaacgggct gcgcggcgcg
     3061 acctcgatcg aggagcgcac cggctacgac ctggcctaca tcgcccactg gtcgctgcgc
     3121 ctcgacctca agatcgtgct cttgaccgcg cgccagatcg tgcgcggcga gaacgcctac
     3181 tgagaccctc actcccagcc cgcacggaga cgtgcgcggg ataggggatg cgtctaccgc
     3241 ggagatctgg gcgtagggca cgacggatcc ctccactacg gtcgggatga cagggagagg
     3301 tcggagcctt tcgggcgatg tgtgtcatca tccgaagccg accctcctcg tccgccccaa
     3361 catggccctg tcatcccgac cgcagtggag ggatccgtcc tcgatggcgc catgccttgt
     3421 cgatgggccg cgtgcccggc ctacggcagc cggcgcaggg ggatgaggct cgagagggcg
     3481 atcagagccg ccagcccgaa ggtgatgaaa taggttccgc cgggaagcag cgcgttgagg
     3541 aagtcggcca gcgggccgaa gaggccggcg accgcgccgc tgccggcggt ggcgatgttc
     3601 gacagcccgt agtactcggc gctgcggccc tcgggtatga cgtccgccat ctgcgcccag
     3661 ttgaccgact ggaagccgcc gaaggcgctg gccacgcagg cgccgaagat cccgaccagc
     3721 cagagccagg gagagaacgc caccccggcc acgccgatgg tgcccagcac ggccatgatc
     3781 cgcagcaccg gcgtccggcc gagacggtcg gagagggcgc cggccaccca ggcggagagc
     3841 gccgccagca cgatggccgc caccaggaag agcgagactt ggctgaggct ggtgatgttg
     3901 aagcggttct gcaggtagta gagaaagagg cgctcgaccg ggtagaggcc gagcaggaag
     3961 aagaagcgcg cgatcaccgc caggcggaag acgcggttgg tcttgaaggc ggcgccgaac
     4021 gagcgaaaca tggcgccgag ctggatatcg cgcaggctgt ggatgcgctc gtcgtcgggc
     4081 ggcggcggca cgtcgggcac ccagcgcagg ttcaggtagg cgaggccgcc gagcagcagc
     4141 gccaggtagg ccagcgccag gtacttgccc cacaacaggt tgaccagcag gccgacgccg
     4201 accacgccga gcgccgcgcc caccacgccc aggccgtcct tgatgcccga ggccaggcca
     4261 cggtcgcgcg gcttgaccag gtcggggatg agcgcctgga acgcggcctg ggccacgttg
     4321 accgccagct gcatcacgat gtaggccacc agcagccaga ggaaggaggg ggccaggctg
     4381 aagagcagca cgcccaggag gccggccgcc gcgccgccgg cgatgaacgt cttgcgccgg
     4441 tgcggcagcg cgaggcggtc gtcgatgcgg ccggcgatcg gctggacgac ggtggcgatg
     4501 ccgatcccca ggaaggtgat cacgcccaag gcgctccctt tgagggaatc gggaaccaac
     4561 tgcgacacct gatcgggcag gatcaggttg ttcagcggcg tccagaagcc gagcagcgcc
     4621 agcgagaaga tgttgatgcg cagcgcgaag aggacggccg cccactgctc cctgcccacc
     4681 agtccgatca accaggagac gaccgggccg tgctgctccc gcgtcgccac gactcagacc
     4741 aaccccagca gcggctggcc gaggaagtag aggctgatgg cgccgatcat gatggtggct
     4801 gcccagccga tgccggtgag taccggcccg atcgggtggc tctgcatcac gcgtgtgtcg
     4861 cgcgccacca gcagcaggaa ggccaggctg atcggcgtgc cgatgccgcc ggcgatgctg
     4921 gagatgtaga ggaggcggat cggcgagacc ccggcgaagg cgaccgcgac cgcgaagagg
     4981 atggcgccgc cgaccgcgat gtagaagcgc cgcgcccgcc ggaccggctc ggagagcccg
     5041 cgccgccagc cgaactcctc accaagcata taggcggtgg tggccgacag caccggcacg
     5101 gccagcacgg ccgaggcgag cagccccacg gcgaagatgt cggacgccag cgcgccggcc
     5161 gccggctcga gcgcccgcgc cgcctgggcg gcggtcgtca ccggctgatg actgacgccc
     5221 agcgtggcgc cggtgctgat caggatgaac cagaagatgg cgacggcgaa gaaggtgccg
     5281 aagccggcgt cggcgcgcgc caggcccagc tcgcggatcg gcaggcgttc ctcggactcc
     5341 tcgatcgact cccagacgta ggcgtagctg gtcagggtcg tacccagcat cgccagcatc
     5401 ccctcgatgt aggcatgctg gaatcggatg tgggggatca gcgtgtgcgc cagcacctgt
     5461 ccccagtctg ggtgcgccag gaaggccgag acgatgtagg ccacgaagac gagcaggatg
     5521 aactccagca cccgcgagat ggcgcggtag ctgccgaaga gcaggagcag caccatcgcc
     5581 aggccaaagg ggaggacgaa ccagcgccag ttgagaccga agagaaggct cagcgccgcc
     5641 gcgcc
//
LOCUS       DASUBI010000559         4166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_569361, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000559 DASUBI010000000
VERSION     DASUBI010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569361"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="cas1c"
                     /locus_tag="VFU72_10745"
     CDS             complement(<1..1017)
                     /gene="cas1c"
                     /locus_tag="VFU72_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HEU5424009.1"
                     /translation="MLNTLYVTTAGSYLHLDHDTVRVEVEHETALRVPLIHLGSIVCF
                     GHVMLSPALLGRCAADGCSVVLLDQNGRFKARVEGPVKGNVLLRRDQHAALSNQALSL
                     EIARSCVAGKVQNSRLVLQRAAREASSGEDAANLSETVSELGALLRDVGAADTLDTLR
                     GREGEAAWRYFGVFGRMIRVDRASFTMRGRSRRPPRDRLNALLSFLYTLLANDCRAAL
                     EGVGLDPQVGYLHALRPGRPALALDLMEELRPVLADRLCLTLINRRQIRERDFVEFTG
                     GAVYLSDEGRKKVIVAYQEKKREEVQHRVIEQKIPLGLVPHAQARLLARHLRGDLGHY
                     PPFLA"
     gene            complement(1023..1676)
                     /gene="cas4"
                     /locus_tag="VFU72_10750"
     CDS             complement(1023..1676)
                     /gene="cas4"
                     /locus_tag="VFU72_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEU5424010.1"
                     /translation="MAGLENDWDIELVPISALEHYSYCPRQCGLIHLEQTFADNTHTT
                     RGQIAHQRVHELGHETRPDVRREFGLPLWSRHLGLVGRADVVEISASGVYPVEHKIGK
                     RRQWGHEAIQLCAQALCLEEMLGQPVPRGAIYYRGSQARREVEFDAELRSLVVAATEG
                     VRAMLRTARLPAAVNDRRCRQCSLNDACLPGATGRERREQAWRAALYRIELPGEEDA"
     gene            complement(1737..2663)
                     /gene="cas7c"
                     /locus_tag="VFU72_10755"
     CDS             complement(1737..2663)
                     /gene="cas7c"
                     /locus_tag="VFU72_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEU5424011.1"
                     /translation="MSMSTTAAPHVDVKRRHDFVLFFDVQDGNPNGDPDAGNLPRVDP
                     ETMHGLVTDVCLKRKVRDYVDVARGNEPGYYIYVQSGEALITKHRRAYEALGLKSTGV
                     KQAQEDVNQARDWMCHHFYDIRMFGAVMSVGVNAGQVRGPLQLTFARSLDQIVPLDLS
                     ITRVAATREEDAKVVVAEGEDEGKGGKQTEMGRKAIVPYGLYRAHGFFNPHLAAQTTA
                     SEDDLALFWQALAGMWDLDRSASRGLMSCRGIYIFSHESPLGNAPAHRLFERVVAARR
                     PEVETPRGFADYEVVVADGDLPMGVTFTRLEA"
     gene            complement(2665..>4166)
                     /gene="cas8c"
                     /locus_tag="VFU72_10760"
     CDS             complement(2665..>4166)
                     /gene="cas8c"
                     /locus_tag="VFU72_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEU5424012.1"
                     /translation="VAGCHRAFVDLTERCLAVTREPSVAAVVRFLHTDPLDALRLPDE
                     FDPGATITFRVHGRSMLEPAEMPIDLASVQSFWAALNDPASAGNGQIMQCLICGQERP
                     VLDRLQGKIKGVPGGQTAGTSIISANEEAFESYGLEASLIAPTCADCGERFTKALNEL
                     LSKRENHIVLGGSVFIFWTRREESTPWFGLLTDPQPEQVRDLLAAVLKTRRTGEFDAT
                     AFYGAVLSGSGGRAVVRDWIDTTVGVAQQRLTAWFAAQRIIDPYGEEGRPLGIYALAA
                     GTVRDLKDVPPTTLRTLLRAALAGTPLPADLLSQAVRRNQAEQGITRQRAALIKLVLL
                     SLEPEQDGGEDSMIKLDPAHPSPAYHCGRLLAVLEEAQRAAIPGIKATLVDRFYGSAS
                     TAPGSVFPRLVRGAQPHLSKLERDREAAYHAIQQRLEDIQGNLTRYPKVLALEEQGLF
                     ALGYYHQRAFDRAQAREAAERRRNAASTAGQQPELESKSAAAPAVEGEN"
BASE COUNT          648 a         1425 c         1384 g          709 t
ORIGIN      
        1 cgccaagaag ggcgggtagt ggccgagatc cccgcgcaag tgccgggcga gcaggcgcgc
       61 ctgggcgtgc ggcaccagcc ccagtggaat cttctgctcg atcacccggt gctgcacctc
      121 ttctcgcttc ttctcctgat aagccacgat cactttcttg cgtccctcgt cgctcaggta
      181 caccgcgcct ccggtgaact ccacgaagtc gcgctcgcga atctgccgcc gattgatgag
      241 cgtgaggcag agccgatcgg ccaggaccgg gcgcagttcc tccatcagat ccagtgccag
      301 cgccggacgg ccgggccgca gcgcgtgcag atagccgacc tgcggatcga ggccgacccc
      361 ttcgagagcc gcgcggcagt cattggccag cagggtgtag aggaacgaga gcagcgcgtt
      421 gagccggtcg cgcggcgggc gccggcttct cccgcgcatg gtgaagctcg cgcggtcgac
      481 gcggatcatc cgaccaaaga cgccgaagta gcgccaggca gcctctcctt cccggcctcg
      541 cagcgtgtcg agcgtatccg ccgcgccgac gtcgcgtagg agcgccccca gctcgctgac
      601 ggtctcgctc aaattggccg cgtcttctcc gctgctagcc tctcgtgccg cccgctgcag
      661 caccaggcgc gagttttgga ctttgccggc gacgcagctc cgggcgatct ccagcgagag
      721 cgcctgattc gagagcgccg cgtgctggtc gcggcgcagt agcacattgc ctttgaccgg
      781 tccttcgacg cgcgccttga agcgcccgtt ctgatcaagc aggacgaccg agcagccgtc
      841 cgcggcacag cgcccgagca gcgccggact cagcatcacg tgtccgaaac acacgatgct
      901 ccccagatga atgagaggca cgcgcagcgc ggtctcgtgc tcgacctcga cgcgcacggt
      961 gtcatggtcg agatgcaggt agcttccggc agtcgtgaca tagagcgtat tcaacatctg
     1021 cgtcacgcgt cctcctcccc gggcaactcg atacggtaga gcgcggcgcg ccaggcctgc
     1081 tcgcgccgct cgcggccggt ggcgccgggt aggcacgcgt cgttcagcga gcactgccgg
     1141 cagcgccggt cgttgaccgc cgccggcagt cgagccgtgc gcaacatcgc gcgcacccct
     1201 tccgttgccg ccaccacgag cgagcgcagc tcagcatcga attccacctc gcgccgcgct
     1261 tgcgagccgc ggtaatagat ggcgccgcgc ggcaccggct gtcccagcat ctcttcgagg
     1321 caaagcgcct gggcgcagag ctgaatcgcc tcgtgacccc actgccggcg cttgccgatc
     1381 ttgtgctcga ctgggtagac acccgacgcg ctgatctcca ccacgtccgc gcggccgacc
     1441 agccccaggt gccgcgacca gagcggcagt ccgaattccc ggcgcacatc ggggcgagtc
     1501 tcgtgaccaa gctcgtgcac gcgctggtgg gcgatctgcc ccctggttgt atgcgtgttg
     1561 tcggcgaagg tctgctccag atggatcaag ccacactgcc gggggcagta gctgtagtgc
     1621 tccagcgccg agattgggac gagctcgatg tcccagtcgt tctccaggcc cgccacgcga
     1681 tgtccttcct gacttgtctg gcggtaggcg ccctcctacc gctgggctgg ctccggctag
     1741 gcttccaggc gggtgaatgt cacccccatc gggagatcgc cgtcggcgac gacgacctcg
     1801 tagtccgcga agccgcgagg ggtttccact tctggccgcc tggcggccac cacgcgttcg
     1861 aagaggcgat gagccggcgc gttacccagc gggctctcgt ggctgaagat gtagatgccg
     1921 cggcacgaca tcagcccgcg gctggcggag cggtccagat cccacatgcc ggccagcgct
     1981 tgccagaaga gcgccagatc gtcctcgctc gcggtcgtct gggcggcgag atgcgggttg
     2041 aagaagccgt gggctcggta gagcccgtag gggacgatcg ctttgcgccc catctcggtc
     2101 tgcttgccgc ctttgccttc atcctcgccc tcggccacga cgaccttggc gtcttcctcg
     2161 cgcgtggcgg cgacgcgcgt gatcgagaga tcgagcggca cgatctgatc gagcgagcgg
     2221 gcgaaggtca gctgtaacgg tccgcgcacc tgcccggcgt tcacccccac actcatgacc
     2281 gcgccgaaca tgcggatgtc gtagaagtgg tggcacatcc agtcgcgcgc ctggttgacg
     2341 tcttcctgcg cctgcttgac gccggtcgac ttcagcccca gcgcctcata cgcgcgccgg
     2401 tgcttggtga tcagcgcctc gccgctctgc acgtagatgt agtagcccgg ctcgttgcca
     2461 cgcgccacgt cgacgtagtc ccgcaccttg cgcttgagac acacgtcggt gacgagccca
     2521 tgcatggttt cggggtccac gcgcggcagg ttgccggcgt ccggatcgcc gttcgggttg
     2581 ccgtcctgca cgtcgaagaa caggacgaag tcatgccggc gcttgacatc gacgtggggt
     2641 gccgcggtcg tgctcatgct catgtcagtt ctccccctcc acggccggcg cggccgcgct
     2701 tttcgactcc agttccggtt gctgacccgc ggttgacgca gcgttccggc ggcgctcggc
     2761 cgcctcgcgc gcctgcgccc gatcgaaggc gcgctggtgg tagtagccca gcgcaaagag
     2821 cccttgctct tccagggcca gcacctttgg gtagcgcgtg aggttgccct ggatatcctc
     2881 cagccgctgc tggatggcgt ggtaggccgc ctcgcggtcc cgctccagct tgctcaggtg
     2941 cggctgtgct ccgcgcacca gccgcgggaa gacggagccg ggcgccgtgg aggccgagcc
     3001 gtagaagcga tcgacgagcg tcgccttgat accgggaatg gcggcccgct gagcctcttc
     3061 gagcactgcc agcagccggc cgcagtggta ggccgggctg gggtgtgccg gatcgagctt
     3121 gatcatcgag tcctcccctc cgtcctgctc cggctcaagt gagagcagga caagcttgat
     3181 cagcgccgcg cgctggcgcg tgatcccttg ctcggcctga ttgcgccgaa ccgcctgaga
     3241 gagcaaatcc gcagggagtg gtgtcccggc cagcgccgcg cgcagcaagg tgcgcagcgt
     3301 ggtcggcggc acatccttca ggtcgcgcac ggtcccagcg gccagggcgt agatcccgag
     3361 cggacgcccc tcttcaccgt acggatcgat gatgcgctgc gccgcgaacc aggccgtgag
     3421 tcgctgctgg gcgacgccga cggtggtgtc gatccagtcg cgcaccacgg cccgtccgcc
     3481 gctgccggag agcacggcgc catagaaggc cgtcgcgtcg aactcgcccg tgcgccgcgt
     3541 cttgagtacc gccgcgagca ggtcgcgaac ctgctctggc tggggatcgg tcaggaggcc
     3601 gaaccacggt gtcgactcct cgcggcgcgt ccaaaagata aagaccgagc cgcccaggac
     3661 aatgtggttc tcgcgcttgc tgagcaattc attgagcgct ttggtgaagc gctcgccgca
     3721 gtcggcgcag gtgggcgcga tcagcgatgc ttccaggcca tacgactcga atgcctcttc
     3781 gtttgcggag atgatcgacg tgcccgcggt ctgcccgccc ggcacgccct tgatcttgcc
     3841 ttgcagccga tcgaggacgg gtcgttcttg gccgcagata aggcactgca tgatctgacc
     3901 gttgccggct gaggcaggat cattgagcgc cgcccagaag gactgtaccg acgcgagatc
     3961 gatgggcatc tcggccggtt cgagcattga acgtccgtgg acgcggaagg ttatcgtggc
     4021 tccagggtcg aattcgtcgg gaagccggag cgcgtcgagc ggatcggtgt ggagaaagcg
     4081 aacaaccgcc gctacgctcg gctcccgcgt aacggccaga caacgttcgg tcaaatcgac
     4141 gaaggcgcgg tgacaccccg cgactc
//
LOCUS       DASUBI010000560         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_655523, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000560 DASUBI010000000
VERSION     DASUBI010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655523"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU72_10765"
     CDS             <1..197
                     /locus_tag="VFU72_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424013.1"
                     /translation="HLYTQRVRSPYERDAACAASPAASHARAMYDRSCHSALNRYDQD
                     VYKDETEVEGSVLDGDNVAR"
     gene            complement(214..1407)
                     /locus_tag="VFU72_10770"
     CDS             complement(214..1407)
                     /locus_tag="VFU72_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5424014.1"
                     /translation="MTVHTGYDLAEARRALRVLRECTYLNTGTVGIMAEPVLAEHLEA
                     ITAFEAGGHLAQSAAREAYERARAALAALIGAQPEEIALTRNATDGVNLIAAGLALAP
                     DTVALTTDQEHPAVILPWALAERHGGAQLRLFRIGATPEETLANFRAALSPAVKLIVV
                     SHVSCETGARLPVAEICRLARERGIWTLIDGAQSVGQFPVDVHEIGCDFMTGNGHKWL
                     CGPKGTGFLYLRQGAISEVEPRHVGDGAVAPNFNRHAMEDAHPALDFEFVASGQRFEF
                     GTRNWHTFAALPAAIDYLAGLGWTAIERHCADLSAALKERLSAAPGVTLHSPRAWEDS
                     SGLVTFAFEGWSGEALSQALWQRYMIIQRRVQIPNGVRISCAYFNDSNDLDRLFAALG
                     DLRAG"
     gene            complement(1545..1754)
                     /locus_tag="VFU72_10775"
     CDS             complement(1545..1754)
                     /locus_tag="VFU72_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424015.1"
                     /translation="MVIAVEPGSGTQRAALQRRQVELYRRLEDGYARIERALEDGQDV
                     TRWEEFWLRLLDEYERLSDELADAA"
     gene            complement(1785..2375)
                     /locus_tag="VFU72_10780"
     CDS             complement(1785..2375)
                     /locus_tag="VFU72_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424016.1"
                     /translation="MSDNTAFEPGKYITKVGNGDYLEVKWRLVWFRSEHPDGAIETEM
                     VSHDGNMAVFRALVRVPNGGSATGWGSESADDFRDYLEKAETKALGRALAALGYGTQF
                     TPDFEFGANDSRVVDSPIDISRTRAARGGLETAPAAATPKQIKFLYAIAREAGLSDED
                     LNAESQELYGKPVDALPRRDASALIERLQQRRAQAS"
     gene            2561..>2709
                     /locus_tag="VFU72_10785"
     CDS             2561..>2709
                     /locus_tag="VFU72_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5424017.1"
                     /translation="MRVTCQQADLQRALGHVSRAVARKSTLPVLGNILFATEGGRLKL
                     AATNL"
BASE COUNT          430 a          892 c          943 g          444 t
ORIGIN      
        1 ctcatcttta tacacaacgt gtacgtagcc catacgaacg agacgccgcc tgtgccgctt
       61 cgcctgctgc ctcgcatgcc cgggccatgt acgaccggag ctgccacagc gccctcaatc
      121 ggtacgacca ggatgtgtat aaagatgaga ccgaagtgga gggatccgtc ctcgacggcg
      181 acaacgttgc gcgttagccg cgcgcgtatc ccatcacccc gcccgcaggt ctcccaacgc
      241 ggcaaacagc cggtcgaggt cattggagtc gttgaagtag gcgcaggaga tgcgcacacc
      301 attggggatc tgcacgcgcc gctggatgat catgtagcgc tgccagagcg cctggctgag
      361 cgcttctccg gaccagccct cgaaggcgaa ggtcaccaga cccgagctgt cctcccaggc
      421 gcgcggcgag tggagggtca cgccgggggc ggcggagagt cgctccttca gcgcggcgct
      481 gaggtcggcg cagtggcgct cgatggcggt ccagccgaga ccggccagat aatcgatcgc
      541 ggccgggagc gcggcaaacg tgtgccagtt gcgcgtgccg aactcgaagc gctgcccgct
      601 ggcgacaaac tcgaagtcga gcgcggggtg cgcatcctcc atggcgtgac gattgaagtt
      661 cggggcgacc gcgccgtcgc cgacgtggcg cggctcgacc tcgctgatgg cgccttgacg
      721 gagatagagg aagccggtcc ccttggggcc gcagagccac ttgtggccgt tcccggtcat
      781 gaagtcgcag ccgatctcat gcacatcgac cgggaactgg cccaccgact gcgcgccgtc
      841 gatcaaggtc caaatgccgc gttctcgggc taggcggcag atctcggcga ccggcagccg
      901 cgcgccggtc tcacaactga cgtggctgac gacgatcagt ttgaccgcgg gcgagagcgc
      961 cgcgcgaaaa ttcgccagcg tctcctcagg ggtggcgccg atgcggaaga ggcggagctg
     1021 agcgccgccg tgtcgctcgg ccagcgccca gggcaggatg acggccgggt gctcctggtc
     1081 ggtcgtcagc gccaccgtgt cgggggcgag cgcgagcccc gcggcgatca gattgacgcc
     1141 gtcggtggca ttgcgcgtga gggcgatctc ctccggctgg gcgccgatga gcgcggccag
     1201 cgcggcacgg gcgcgctcgt acgcctcacg cgcggcggac tgcgccaggt ggccgcccgc
     1261 ctcgaaggcg gtgatggctt ccaggtgctc ggccagcacc ggctcggcca tgatgccgac
     1321 cgtgccggtg ttaaggtagg tgcactcgcg cagcacgcgc aatgcgcggc gcgcttcggc
     1381 caggtcatac ccagtatgga cagtcacggc gtctgctcct ccggtcgtca ccagtcgcgg
     1441 cggccgtatg ccagcgggcc gctgtggtgc ctcttgtagc agacgcgaag cggggagaaa
     1501 aggcgggggc gggagccggc gccatgccgg ctccgatcta tacgctaggc agcgtcggct
     1561 agctcatccg agaggcgctc gtactcgtcg agaaggcgca gccagaactc ttcccagcgg
     1621 gtgacgtcct gaccatcctc cagcgcgcgt tcgatgcggg cgtaaccgtc ctccagccga
     1681 cggtacagct cgacctggcg ccgctggagc gccgcccgtt gggtcccgct gccgggctcg
     1741 accgcgataa ccatcttctg cctcctcgac gactgcacga ttcgctagga cgcctgggcg
     1801 cggcgctgct gcaggcgctc gatcagcgcc gaggcatcgc ggcggggcag ggcatcgacc
     1861 ggcttaccgt agagctcctg cgactcggcg ttcaagtcct cgtcgctgag acctgcctca
     1921 cgcgcgatgg cgtagaggaa cttgatctgc ttgggcgtgg ccgcggccgg tgccgtctcc
     1981 aggccaccgc gcgcggcgcg ggtgcgactg atatcgatcg gcgaatcgac tacgcggcta
     2041 tcgttggcgc cgaactcgaa gtcgggcgtg aactgggtgc cgtagccgag cgcggccagg
     2101 gcccggccga gcgccttggt ctcggccttc tccaggtagt cccggaagtc gtcggcgctc
     2161 tcgctgcccc agccggtggc gctgccgccg ttgggcacgc gcaccagggc gcggaagacc
     2221 gccatgttgc cgtcgtggct gaccatctcc gtctcgatcg cgccgtccgg gtgctcgctg
     2281 cggaaccaga ccagccgcca cttgacttcc agatagtcgc cgttgccgac cttggtgatg
     2341 tacttgccgg gctcgaacgc ggtgttgtcg ctcatcgtag ccatctcctt gcttgctcac
     2401 cggattccga acatctgttc taattgtagc agaaggtgtc gcgttgtcaa tcacctcgag
     2461 cgggatcgtg cccgccgcgg tccccggggc acgccgcggg gtatgctata atccgggcac
     2521 gagaacgtgt acggacacat gagtgaagag gggtggaagc gtgcgggtca cgtgccagca
     2581 agcggatttg caacgagcac tgggtcatgt ctcccgcgcc gtggccagga agtcgaccct
     2641 gcccgtgctg gggaatatcc tgttcgccac cgagggggga cggctgaagc tggcggcgac
     2701 caacctgga
//
LOCUS       DASUBI010000561         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_320233, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000561 DASUBI010000000
VERSION     DASUBI010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320233"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFU72_10790"
     CDS             <1..245
                     /locus_tag="VFU72_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424018.1"
                     /translation="RVMAADLRGTSVTVNILMPGGVTETGMLPPGHKPEGLRPIDPAV
                     MGPPIVWLASPAADGMHDERIVATEFESWLAQRRGT"
     gene            complement(258..1859)
                     /locus_tag="VFU72_10795"
     CDS             complement(258..1859)
                     /locus_tag="VFU72_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5424019.1"
                     /translation="MVETAKSQPATAAPSASDADLIAAARSGDAGAYGVLYERHAQAA
                     RRLARHIVTTSVDADDVVAETFTRVLYAIRNGNGPIEAFRPYLFTAVRRVAIDLVSGQ
                     RRQIPTDDTDMPDPGEPFDDPMVAGLERSLISRAFWSLPERWRAVLWHTEIEQTKPAE
                     AAALLGLSPNGVSVLKRRAREGLRQAYLQLHLSDRTKAGCAPIAAKLGRYVRGGLSRR
                     QAREIEDHLARCADCTAASADLASINDTLRGVLAPVVLGGAAAGYLASAGQAHTATAA
                     AAARLHVGLHALRPATRRAAEAITHHPIASAAAVATAAAITVPSLYFPLHAHPKAAPT
                     PGQTHFHGAHGGARYAQGVRPPVGAGPIPTLVASPVPTTTTTGGASPTPTRSPSRSGN
                     PTPSGSPTSTPSPKPTPTVSATAKLSVSVQVAGLLNLGVTVLVTVDVSDPGSAATGPV
                     TANITLPSGVSLLGLVGSSSWSCSATSGGQTCSHGPIDAGGTSSLSFDVLVANLTGCG
                     NAIIATVVSGSLSATGASATKVQCL"
     gene            complement(1882..>2069)
                     /locus_tag="VFU72_10800"
     CDS             complement(1882..>2069)
                     /locus_tag="VFU72_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424020.1"
                     /translation="GGISQAYLDHGYAAMAATPGAVCCSSCRPPSLSPCPRIGKDDFN
                     EVALLIGMVGDDNSIDA"
BASE COUNT          331 a          663 c          746 g          329 t
ORIGIN      
        1 cccgggtcat ggcggccgac ctgcgcggca ccagcgtcac cgtgaatatc ctgatgcccg
       61 gcggcgtcac cgagaccggc atgctgccgc ccgggcacaa gccggaaggc ctgcggccga
      121 ttgaccccgc ggtcatgggc ccgccgatcg tctggctggc ctcaccagcc gcggacggca
      181 tgcacgacga acggatcgtc gccaccgagt tcgagtcgtg gctagctcag cgtcgcggaa
      241 cttagcgcgt agccccttca gaggcactgg accttggttg ccgacgcgcc tgtagccgac
      301 aggctgccgc tcaccactgt ggcgatgatc gcgttaccgc atccagtcag gttcgcgacc
      361 aggacgtcga atgacaggct cgacgttcct ccggcgtcga ttgggccgtg cgagcaggtc
      421 tgtcctccgc ttgtcgccga gcacgaccag gatgacgagc cgaccagccc gagcaggctg
      481 acgccggagg gcagcgtgat gttcgcggtg accgggccgg tggccgcgct gccgggatcg
      541 gacacgtcga cggtcacgag cacggtgacg ccgaggttga gcaggccggc tacctggacc
      601 gacacgctga gcttggcggt agcggagact gtcggcgtcg gtttcgggct tggtgtgctg
      661 gtcggcgagc cagatggagt cgggttaccc gaccgcgacg gcgatctcgt cggcgtaggc
      721 gaagcgccgc cggtcgtggt agtggtcggc acgggcgacg cgaccagagt gggaataggc
      781 cccgcgccga cgggcggccg gacgccttgg gcatagcgtg cgccgccatg agcgccgtgg
      841 aaatgtgtct gcccgggagt tggtgccgcc ttggggtgcg catggagcgg gaagtacagg
      901 ctgggcaccg tgatagcggc ggccgtggcc acggcggcag cggaggcgat cgggtgatgc
      961 gtgatcgcct ctgctgcccg gcgggtggcc ggtcgaaggg cgtgcaggcc gacgtggagc
     1021 cgggcggcgg ctgccgccgt ggcggtatga gcctgcccgg ctgaggctag gtaacccgcc
     1081 gctgccccgc cgagcaccac tggtgcgagc actccgcgca gcgtgtcgtt gatggaggcg
     1141 aggtcggcgc tagccgcggt gcagtcggcg cagcgggcga ggtggtcttc gatctcgcgg
     1201 gcctgtcgcc tcgacagccc gccgcgaaca tagcggccga gcttcgcggc aatgggggcg
     1261 cagccagctt tggtccgatc ggacaggtgc agctgcaggt aggcctgcct caagccttcg
     1321 cgggcacgtc gcttgagcac cgagacaccg ttaggcgata gcccgagcag cgccgccgcc
     1381 tcggctggtt tcgtctgctc gatctcggtg tgccacagga ccgcgcgcca gcgctcgggc
     1441 aatgaccaga acgctcgcga tatcaatgac cgctcaagcc ccgcgaccat cggatcatcg
     1501 aaaggctcgc cagggtcggg catgtcggtg tcatcggtag gaatctgccg gcgctgaccg
     1561 ctgaccaagt cgatggccac gcgccggacc gccgtgaaca gatagggcct aaatgcctcg
     1621 ataggcccgt tgccgttcct gatcgcgtaa agcacccgcg tgaacgtctc agcgacgaca
     1681 tcgtcagcat cgaccgacgt agtgacgatg tgacgcgcca gacggcgtgc ggcctgggca
     1741 tgccgctcgt aaagcacgcc gtaggcgccc gcgtcgccgg accgggcggc ggctatcagg
     1801 tcggcgtcgc tggccgacgg agccgccgtg gcgggttgag attttgccgt ttctaccaac
     1861 gagctatcca tatttcgcgg atcaggcgtc gatggagttg tcatctccta ccatcccgat
     1921 cagcagagca acttcattga aatcgtcttt cccgatgcga gggcagggcg agagggaagg
     1981 cggtcggcac gacgagcagc agacggcgcc cggcgtcgcc gccatagccg cgtatccatg
     2041 atctaagtat gcctggctga tcccgccag
//
LOCUS       DASUBI010000562         7176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_569318, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000562 DASUBI010000000
VERSION     DASUBI010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569318"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFU72_10805"
     CDS             <1..630
                     /locus_tag="VFU72_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424021.1"
                     /translation="NVDYQKEYPFDVAGAKKLLDDAGWAPGSDGIRAKGGQRMSFTML
                     TYSGFTEYINDQAILQQALKDIGIEVKPSVVEYNTLQGMWHDANENPDDRAMEVQEWP
                     HPFEQDPDLFNELDSKNFPPGDNYEYFKNDDVDKLIEQGRSTVDPAARVKIYQQLDVA
                     RLKSLPALPLYCAVDGWVVSKRLGGIPADTPSFRWYQRAFPEKISKLSK"
     gene            666..1646
                     /locus_tag="VFU72_10810"
     CDS             666..1646
                     /locus_tag="VFU72_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424022.1"
                     /translation="MTGYVIRRVLWMVPLLLGVSIITFAMLKHAPGDPVAAILAQART
                     NGAQLTAADRERLRKQYGLDKPAYEQYVDWLGLVLHGNLGTSTRMNQPVTHVIGQRLP
                     NTMKLAGVSLVLTLLIAIPLGIISAVKQYSLLDYVLTSFNFIGISLPQFWLALMLLFF
                     LAVKVGWFPVNGMHSAYVQGGFWAHFKDSALHFVLPVISITLTGLASYMRYQRASMLE
                     VLNHDFVRTARAKGVGERSVILLHAWRNSLIPIITLLGYVFVILVEGSVVVETIYSWP
                     GMGSLAVESLTQRDYPMVMGIVLLSAALILLGTLVSDLLYALADPRVRYD"
     gene            1668..2594
                     /locus_tag="VFU72_10815"
     CDS             1668..2594
                     /locus_tag="VFU72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424023.1"
                     /translation="MSAQAQTAETVSWDRATRGSVRSPWRMTVRRFARHRLALVSAFF
                     LLILFLVAALAPLLAPYDPNKTNLQLAKFGRPASPSLSHPMGSDQLGRDWLSRLTIGS
                     RVSLIAGFLATGFAIVVGTLLGAIAGYAGRGVDMLIMRLSDVLLAFPPLLFLMGALTG
                     FKNRPVWLIALVIGVIGWMNVARLVRAEFLSLRRREYAEAARAIGARPAAIIFRHLLP
                     NATSPIIVAATLGIPAAILTESTLSFLGFGIQPPTASWGNMLQIAYVQMRDNQAWWIG
                     FYPGLMIALTVLAFNFVGDGLRDALDPRTWTR"
     gene            complement(2620..2829)
                     /locus_tag="VFU72_10820"
     CDS             complement(2620..2829)
                     /locus_tag="VFU72_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424024.1"
                     /translation="MSPKWGTILAEVGFLLIMIAGVWLVASEIGAFKHSTFRATVAGI
                     GLALAGLLLIIATRWGHFGQGPSAR"
     gene            complement(3263..4573)
                     /locus_tag="VFU72_10825"
     CDS             complement(3263..4573)
                     /locus_tag="VFU72_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424025.1"
                     /translation="MKLRLPSALASLAYRDFTLLWLGQFISTSGTQIQTVALGWLVYT
                     LTGSAALLGGIALARAIPTILLSLFGGTLADQMDRRRLLLVSQSVLAGFSALLAVAIQ
                     FGLASVTILYLFAILTAAASAIDAPTRQAFIPALVPRERLANALTLNVLIFNVAAVLG
                     PAFGGLVISGLGVEAAYWIDAGSFFAVVAALVLMRARIPPAVLPRRGLAALVDGLQFV
                     RARPILWQLMLIDFFAVLFASRFGLLPVFAVSVLKIGAGGLGFLYAAVSFGAVLGSVL
                     FAFVPQPRRPGFLVAVTVIGYGGVLALFGLARSLPLALFLLATSGALDAVSSALRQTT
                     RQLATPDSLRGRVGALSSVFAAGGPRLGDFQAGVLASLLGAGSAMVIGGAACLAMAVG
                     SRWWAPQLWSYHGEELRTGEPQVVSHGTVTERMREAAESISQSE"
     gene            4915..5607
                     /locus_tag="VFU72_10830"
     CDS             4915..5607
                     /locus_tag="VFU72_10830"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU5424026.1"
                     /translation="MHTAQRFNPGRLLVGLLLLAVGSILLLDRAGWGDAGHLIGQWWP
                     LIIVAAGLIQIAVANRPAPGPVLLVLFGLVLLPFTLDLISASFWSVFWPLVLILVGLA
                     FIFRQGLWPRARTSASDDVSAFAIFSPSTVVSHAAALQGGTATAIFGGVTLDLRQARL
                     APGAAIDASAAFGGIDVLVPPGWRVEMRGLPIFGGLSDKTRHEGPLPDDAPALTVNGL
                     AFFGGVDVKNEK"
     gene            5642..6616
                     /locus_tag="VFU72_10835"
     CDS             5642..6616
                     /locus_tag="VFU72_10835"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5424027.1"
                     /translation="MRRIGVLTSGGDAPGMNAAIRAVVRSGLDRGWEVFGIRRGYRGL
                     IDADAELLGPRDVGGIIHRGGTILGTSRCPEFMEQAGQRQALRSLERLGLDGLVIVGG
                     SGSQVGTLALARAGAPAVGVASTIDNDLAGIDITLGVDTALNIALEAIDRLKVTASSL
                     GRAFLVEVMGRECGYLALMAALAGGAEAVILPGVACEPEALAATIDAAYERGKAHAIV
                     VVAEGADYNAERLVNYFKENQRRLGFELRATILGHVQRGGAPGAFDRLLGTRSGAGAV
                     EQLAQGAYGRVVGLRQGRIAAVPLDEVVLKRPRLDPELVELAAALARQ"
     gene            complement(6986..>7176)
                     /locus_tag="VFU72_10840"
     CDS             complement(6986..>7176)
                     /locus_tag="VFU72_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HEU5424028.1"
                     /translation="TRAGGTLPVSGIFLSELGAETVSFAWGMPGGNVHAPNENYSLKD
                     SFEPSRRAYCMYLNALAR"
BASE COUNT         1136 a         2485 c         2480 g         1075 t
ORIGIN      
        1 aacgtggatt accagaagga gtaccccttc gacgtagccg gggccaagaa gctgctcgac
       61 gacgccggtt gggcgccggg cagcgacggc atccgcgcca agggcggcca gcgcatgtcc
      121 ttcaccatgc tgacctactc cggcttcacc gagtacatca acgaccaggc aatcctgcag
      181 caggcgctga aggacatcgg catcgaggtc aagccgagcg tggtcgagta caacacgctg
      241 cagggcatgt ggcacgacgc caacgagaat ccggacgacc gcgccatgga ggtccaggag
      301 tggccgcacc ccttcgagca ggatcccgac ctcttcaacg agctggactc caagaatttc
      361 ccgccgggcg acaactacga gtatttcaag aacgacgacg tcgataagct gatcgagcag
      421 gggcgcagca ccgtcgaccc ggccgcgcgc gtcaagatct accagcaact cgacgtggcc
      481 cggctgaagt cgctgccggc gctgccgctc tactgcgcgg tcgatggctg ggtcgtcagc
      541 aagcggctcg gcggcatccc ggccgacacg ccgagcttcc gctggtacca gcgtgccttc
      601 ccggagaaga tctcgaagct ctcgaagtag acaggcatcc cagccggacg ggatgaggag
      661 cgagcatgac cggctacgtc atacggcggg ttctttggat ggtgccgctg ctgctcggcg
      721 tctcgatcat caccttcgcc atgctcaagc acgcgccggg cgaccccgtg gccgccatcc
      781 tggcccaagc gcgcaccaac ggcgcgcaac tgaccgccgc cgaccgcgag cgcctgcgca
      841 agcagtatgg gctggacaag cccgcctacg agcagtacgt cgactggctc gggctggtgc
      901 tgcacggcaa cctcggcacc tccacccgca tgaaccagcc ggtgacgcac gtcatcggcc
      961 agcggctgcc gaacaccatg aagctggccg gtgtctcgct ggtgctgacg ctcctcatcg
     1021 ccatcccgct ggggatcatc tcggccgtca agcagtactc gctgctcgac tacgtgctga
     1081 ccagcttcaa cttcatcggc atctcgctgc cccagttctg gctggcgctg atgctcctct
     1141 tctttctggc ggtcaaggtc ggctggttcc cggtcaacgg catgcacagc gcctacgtgc
     1201 agggcggctt ctgggcgcat ttcaaagaca gcgccctcca cttcgtgctg ccggtcatct
     1261 cgatcacgtt gaccggcctg gcgagctaca tgcgctacca gcgcgcctcg atgctggagg
     1321 tgctcaatca tgacttcgtg cggacggccc gcgccaaggg ggtcggcgag cgctcggtga
     1381 ttctcttgca cgcctggcgc aactcgctga tcccgatcat caccctgctc ggctacgtct
     1441 tcgtgatcct ggtcgagggc tcggtggtgg tcgagacgat ctactcctgg ccgggcatgg
     1501 gcagcctggc ggtcgagtcg ctgacgcagc gtgactaccc gatggtgatg gggatcgtgc
     1561 tcctgtcggc ggcgctgatc ctgctcggca cgctggtctc cgacctcctc tacgccctgg
     1621 ccgacccacg cgtccgctac gactaacgac gaggagcagc cgcggcgatg agtgcgcaag
     1681 cgcagacggc cgaaaccgta agctgggacc gggccacacg cgggagcgtg cgcagtccct
     1741 ggcgcatgac cgtgcgccgt ttcgcccggc atcgtctggc gctggtcagc gcgttcttcc
     1801 tgctcatcct cttcctcgtg gccgcgctgg cgccgctgct ggcgccctat gacccgaaca
     1861 agaccaacct gcagctggcc aagttcggcc gcccggccag ccccagcctg tcgcacccga
     1921 tggggagcga ccagctcggc cgcgactggc tctcgcgtct gaccatcggc tcgcgggtct
     1981 cgctgatcgc cggcttcctg gccaccggct tcgccatcgt ggtcggcacg ctgctggggg
     2041 cgatcgccgg ctatgccgga cgcggggtgg acatgctgat catgcgcctc tccgacgtgc
     2101 tgctggcctt tccgccactg ctcttcctga tgggcgcgct gaccggcttc aagaaccgcc
     2161 cggtctggct gatcgcgctg gtgatcgggg tgatcggctg gatgaacgtg gcgcggctgg
     2221 tgcgcgccga gttcctcagc ctgcgccggc gtgagtacgc cgaggcggcg cgcgccatcg
     2281 gggcgcggcc ggcggcgatc atcttccgcc acctcttgcc gaacgcgacc agcccgatca
     2341 tcgtggccgc cacgctcggc atcccggcgg cgatcctgac cgagtcgacc ctctccttcc
     2401 tcggcttcgg catccagccg ccgaccgcct cctggggcaa catgctccag atcgcctacg
     2461 tgcagatgcg cgacaaccag gcctggtgga tcggcttcta cccggggttg atgatcgcgc
     2521 tgacggtgct tgccttcaac ttcgtcggcg acggcctgcg cgacgcgctc gacccccgca
     2581 cctggacgcg gtgaggcgct gacgcgccgt cgctctcgct catcgcgcgc ttggcccctg
     2641 accaaagtgg ccccagcgcg tggcaatgat cagcagcagg ccagcgagtg cgagcccgat
     2701 cccggcaacg gtcgcgcgga aggtgctgtg tttgaacgcc ccgatctccg aggcgaccag
     2761 ccaaaccccg gcgatcatta tgagcaggaa ccccacttcg gccagaatgg tgccccattt
     2821 cggcgacact gatcctcgct tcttccctcc gagcatcgat cagctctcgt ccatcaatca
     2881 cgtgcgtccg agatcggctc cagcatcgca ccggtgccac agtaggggca ggtcacaaag
     2941 cgccggcccc cggagcgacg atataagaag atctccgcga agaggtacga cgagtaggac
     3001 tgcttgaagg agcgcccgca ggtgggacaa acgaagtgcg tggtccgagt ccgcagaaat
     3061 acgtagacca tcgccacggg gatggcgaga aggatgagga tcggaggcgc gctggtcatc
     3121 acacacccct actgacaaga ttccagtcag gagtccggcc gcggtgcacg cccgcattct
     3181 accctgctgc ccactataga acccgcccgg acaaccatct atgaggaagc acaagagcgc
     3241 ttcgccggcg agcatacgcg cctcactcgc tctgggagat cgactcggct gcctcgcgca
     3301 tgcgctcggt cacggtgccg tggctgacca cctgcggctc gccggtccgg agttcctcgc
     3361 cgtggtagct ccagagctgg ggcgcccacc agcgcgagcc gacggccatt gcgagacaag
     3421 ccgccccgcc gatgaccatc gcgctgccgg cgccgagcag gctggccagc acgccggcct
     3481 ggaagtcgcc caggcgcggg ccgccggcgg cgaagaccga ggagagcgcg ccgacccgcc
     3541 cgcgcaggct gtcgggggta gcgagctggc gcgtggtctg gcgcagggcc gagctgacgg
     3601 cgtcgagcgc gccggaggtc gccaggagga acagcgccag gggcagcgag cgcgctagcc
     3661 cgaagagcgc cagcacgccg ccgtagccga tcaccgtcac cgctaccagg aagccgggac
     3721 ggcgcggctg cggcacgaag gcgaagagga ccgagccgag caccgcaccg aagctgaccg
     3781 ccgcgtagag gaagcccagc ccgccggcgc cgatcttgag cacgctgacc gcgaagaccg
     3841 gcagcaggcc gaagcgcgag gcgaacagga ccgcgaagaa atcgatcagc atcaactgcc
     3901 agaggatcgg gcgggcgcgc acgaactgca ggccgtcgac cagcgccgcc aggccgcgcc
     3961 ggggcagcac ggcgggcggg atgcgcgcgc gcatcagcac cagagcggcg acgacggcga
     4021 agaacgagcc ggcgtcgatc cagtaggcgg cctcgacgcc cagcccgctg atgaccagcc
     4081 cgccgaacgc cggaccgagg accgcggcca cgttgaagat cagcacgttc aaggtcagcg
     4141 cgttggccag ccgctcgcgc ggcaccagcg ccgggatgaa ggcctgccgg gtgggcgcgt
     4201 cgatggccga ggcggcggcg gtcaggatgg cgaagaggta gaggatggtg acgctggcca
     4261 gcccgaactg aatcgcgacc gccagcagcg cgctgaagcc cgccagcacg ctctgggaga
     4321 cgagcagcag gcgccgccgg tccatctggt cggccagcgt gcccccgaag agggagagaa
     4381 ggatggtggg gatggcgcgc gccagggcga tgccgcccag cagcgccgcc gagccggtca
     4441 gggtgtagac cagccagccg agcgccaccg tctggatctg ggtgccgctg gtggagatga
     4501 actggcccag ccagaggagg gtgaagtcgc ggtaggccag ggatgccagt gccgagggga
     4561 ggcgcaactt catcgtgccg cgcctgcccg gcgtgcgcta tcccagccag cgtgattcat
     4621 cccgtgtccg gctcctccca gccccctacc cgcgctaaac gggaatgcta gcagacccgg
     4681 gccggcggcg ggaaccaagt cgccagggcc agcgaggttg gctgtggcgc gccgcacggc
     4741 gcctcacggc gcggcgacac ggagctgtaa cacccaggcg gagaatgcga tgccgccgac
     4801 acccggttgg cttcatgctt ggtctggacg gcacgcccgt cccgtcgggc gtgcacaacg
     4861 tcacgggctg tcgcccgccc aacagcgcgc ggcgcgtgtg gagaggagcg gtcaatgcat
     4921 accgcacagc gattcaaccc cggacggctg ctggtgggcc tgctcctgct ggccgtgggt
     4981 tcgatactcc tgctcgaccg ggcgggctgg ggcgacgccg gccacctgat cggccagtgg
     5041 tggccactga tcatcgtggc cgccggtctc atccagatcg ccgtcgccaa tcgcccggca
     5101 cccgggcccg tgctgctggt cctcttcggg ctcgtcctgc tgcccttcac gctggacctg
     5161 atctcggcgt cgttctggtc ggtcttctgg ccactggtgc tgattctggt cggcttggca
     5221 ttcatcttcc ggcaggggct gtggccgcgg gcacgcacgt ccgccagcga cgacgtcagc
     5281 gccttcgcca tcttcagccc cagcaccgtg gtcagccacg cggccgcctt gcagggtggc
     5341 acggctaccg cgatctttgg cggcgtgacg ctcgacctgc gccaggcgcg gctggcgccg
     5401 ggcgcggcga tcgacgcgtc ggccgccttt ggtgggatcg acgtgctggt gccgccgggc
     5461 tggcgggttg agatgcgcgg cctgccgatc ttcggcggcc tatccgataa gacccgacac
     5521 gagggcccct tgccggacga tgcgcccgcg ctgacggtca acggattggc gttcttcggc
     5581 ggagtcgacg tcaagaacga gaagtagcgg gacgcgcgct gtctcggaag atggaggcgg
     5641 gatgcggcga atcggggtct tgaccagcgg cggcgacgcc ccgggcatga acgcggcgat
     5701 ccgcgcggtc gtccgcagcg ggctcgatcg gggctgggag gtcttcggca tccggcgcgg
     5761 ctaccgcggg ctgatcgacg cggatgccga gttgctgggg ccgcgcgatg taggcggcat
     5821 catccaccgc ggcggcacga tcctcggcac atcccgctgc cccgagttca tggagcaagc
     5881 ggggcagcgg caggcgctgc gctcgctgga gcggctcggc ctcgatggac tagtcatcgt
     5941 cggcggcagc ggcagccagg tgggcacgct ggcgctggca cgcgcgggcg cgcccgccgt
     6001 cggcgtcgcc tccaccatcg acaacgatct ggcgggcatc gacatcacgc ttggggtgga
     6061 caccgcgctc aatatcgcgc tggaggcgat cgaccgcctc aaggtgacgg cgtcgtcgct
     6121 cgggcgcgcc ttcctggtgg aggtcatggg gcgggagtgc ggctacctgg cgctgatggc
     6181 ggcgctggcc ggtggcgccg aagcggtcat cctgcccggg gtcgcatgcg agcccgaagc
     6241 cctggcggcg acgatcgacg cggcctacga gcggggcaag gcgcacgcca tcgtggtggt
     6301 cgccgaaggc gccgactaca acgcggagcg tctggttaac tacttcaagg agaaccagcg
     6361 gcggctcggc ttcgagctgc gagctaccat cctcggccac gtccagcgtg gcggggcgcc
     6421 gggcgccttc gaccgcctgc tgggcacccg ctccggcgcc ggcgcggtgg agcaactggc
     6481 gcagggcgcg tatggccgcg tggtcggact gcgccagggg cggatcgccg ccgtgccgct
     6541 ggacgaggtg gtcctcaagc gcccgcggct cgaccccgag ctggtggagc tcgccgccgc
     6601 gctcgcgcgc cagtgacgcg gacgagcaac cagcagaccg cgcgctaccg cgaagcaccc
     6661 tgctctcgtt gggtgcttgc cgaaccctgt cgtcccgcgc ccaacgttct cagttgaccc
     6721 cgttgccggt gagtgggggg aagagggcgg ccacggaggg ccgccgctac aggtgatatg
     6781 cgtgccgttt cccctcttgc cggtgcgccg gtgtacgcgt cgcttcctcc cccggtagga
     6841 gcaggcacgg gggcctgccc ttcccccagg gccggcccgc acgtttgaca cccacgccgc
     6901 ccagttgctc tgtgcctccg ttgccggtac gcgggggccg cccctaccgg aggaacgcgc
     6961 gccacccgcg gtcgacccaa gccggtcagc gggccagtgc gttgaggtac atgcagtagg
     7021 cgcgccggga cggctcgaag gagtccttga ggctgtagtt ctcgttcggc gcgtgcacgt
     7081 tcccgccggg catgccccag gcgaacgaca cggtctcggc tcccagctca ctcaggaaga
     7141 tgcccgagac cggcagcgtg ccgccggcac gggtgt
//
LOCUS       DASUBI010000563         7141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_111864, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000563 DASUBI010000000
VERSION     DASUBI010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111864"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFU72_10845"
     CDS             complement(<1..836)
                     /locus_tag="VFU72_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5424029.1"
                     /translation="MAARQPLNRLVAAPIQPIQVPLNLGAARAGVEQGAAALAAALRA
                     RWAGEDARSHALLARLRPSEVIPVAPLALGTPGLQPGGALYQGEIVAAAEKLAERVAA
                     AIARGELPLALGGDHALSLGSIAGAARAAERLAVLWIDAHADLNWPEVSPSGRIHGMI
                     LGASLGRGPRALTGLAGPAPKVRPEDAYLFGLCLLDPGERAWIAEGAIQAVTVDDIDE
                     QGLDAAFERIACDIARSGADALHVSLDVDVLNEQVMPGTGSKHWGGLTARELARVIRR
                     LRA"
     gene            complement(836..3007)
                     /gene="argS"
                     /locus_tag="VFU72_10850"
     CDS             complement(836..3007)
                     /gene="argS"
                     /locus_tag="VFU72_10850"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5424030.1"
                     /translation="MFHNEEQRAAALINDALETLGYGRRRFEMRAIPFSGVWGTSSSV
                     SYQIANDLLMAESAEELAGLSKKEAKRRVKELVSARAQEIAGQVAGRLAQDGFAAVEA
                     LNGYINVIFDTNHFSNVVVRSVVDAGADYGRGPAQTDRVMVEFSQPNTHKAFHIGHLR
                     NACLGSALSRILRFAGFDLLEANYIGDIGMHVIQTLWCYRRFHQGEEPPPVERGRWLG
                     QVYTEAVQRLNYRQDLAAFLNMLSREDLAFVERIDRMMKELWKTHKIGEDVAYLLGQL
                     AHQRELKPDAFYDNETIPQFWPIAGKELAQELADAESAVLAPPESEEAEAAAREAVVA
                     GRARLEQWERLATHLDWWAHVPEWQQEVRETFQRWEEKDPAFVALWEETKAWSMEDFQ
                     RIYEQLGVHFDVWFYESQVEERGREIVRELLERGIAEISDGLPVVKIDERLGLEKETY
                     RTLPILRSDGTTLYSTKDLALTKEKFEQYGIDRSIWVVDVRQSLYFQQIFKIMELWGF
                     EQAEKCFHLSYETVMLPSGAMSSRSGNTILYDDVAEAVLTRAREIIDEKNPELAPELK
                     ARIAHDVGIGSMKYGMLARDNNRVIVFDIDEALSFEGHAAPYIQYAHARACRILEKVE
                     RLPEGELIFGEPTPEETNLIEEIALFPSEVERAASEYKPLLIANYVYELAKRFNDFYR
                     ACPVLSAPEPQRQARIALVAAARQTLANGLNLLGIAAPEIM"
     gene            complement(3128..3505)
                     /locus_tag="VFU72_10855"
     CDS             complement(3128..3505)
                     /locus_tag="VFU72_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424031.1"
                     /translation="MRKGWLYLIVIGLIVLFVVLFVVLYLLGSSSESALQKITEISII
                     FMALSMILVILLLGALVGAVLWLVLLLKDKVIPLLEQLTLAASRVRGTAEFVGEEVAS
                     PIISAYSTLAGVRAMIKTVTGKK"
     gene            complement(3531..3869)
                     /locus_tag="VFU72_10860"
     CDS             complement(3531..3869)
                     /locus_tag="VFU72_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424032.1"
                     /translation="MSPTLILALTLGSIAGLLAHALLGRRLWQLPCFWLAGVGGFLAG
                     EIVAILAGVEFFRLGSIPLPAALAGSLLGAVICWFFTAPHEPRPRRRVRRVARAADAR
                     QAATDPRGAR"
     gene            complement(4011..5726)
                     /locus_tag="VFU72_10865"
     CDS             complement(4011..5726)
                     /locus_tag="VFU72_10865"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEU5424033.1"
                     /translation="MQSARRIYVYFIAAVSLAVWAFGLMNLLRLALEQFAEALGWREV
                     IGRTPGDLRREVSMYAALVVVALPIWLLHWWLAERAVAQGETGEAERQSVVRTFFFAA
                     VLLVSFWMWVSPAVDLVRFAVLEIGHARETFSGGVPGALAAVIVTSLVWIYHVRMRLR
                     DLRRGTIAMDATWHPRLYRYIALSAGAALLIVGAVQLLDLIGDAVFGTQGVIFAGERW
                     WLPRLADGAASVVAGLAVWSLHWWYTQSVLERGDWFSLSERQSTLRRIYLYGVILVTS
                     VLTLLALSSSLETVLRALLSVPVSSGARAFLRRLIEPPVGAIPFVAFWLFYRQRIRDE
                     ARRFGSAAFQDSVSRFYRYGLSLVGLAFTALGAAYLAGILVDLVLGGSRTVGFSAAWW
                     RGQVSQYGAMALVGGAVWLWHWYAIQARRQANPERERWTTSRRFYLYAVLGGSLIATL
                     ASFSVILYRLFNLLLNVTPAGGFVSAISAALGVAVIAVALLVYHGLILRRETLARPAL
                     ETATRAALHLVLSGPTGADFDAELDALRAQLHPGFELHAAAATSRQAPSASPSPEGGT
                     RATAG"
     gene            complement(5731..6282)
                     /locus_tag="VFU72_10870"
     CDS             complement(5731..6282)
                     /locus_tag="VFU72_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424034.1"
                     /translation="MTSQTATGHAGRTIAIIGGLVLLLVIAAVVATLALDAGKPASYP
                     AGSPEYAFQRYYQAFQTGDMQAAYASFSQRVRDQLSYDQYVWYATKFQPPLEQSTVVR
                     IDHVNRTNGVVTLYLAVERASGSGLDVSRYTEQRSIRLVEEAGGWKIDEPLAGIEVIY
                     ALPPPAAPTATPSADLASERGRG"
     gene            complement(6408..>7141)
                     /locus_tag="VFU72_10875"
     CDS             complement(6408..>7141)
                     /locus_tag="VFU72_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424035.1"
                     /translation="PVARELGAEWGVLEPLQTEASLDGQVAELRALLLAHARLPVTLV
                     GSSWGAMLSLILAARHPELVARLILVGSGVYDARYAQGIEQTRLERLSAAERREIDAL
                     LISLNGPAGAGRDDVLRRLGAIFARADSYDPLPHDDELLEVRFDVFQQVWGEAEALRA
                     SGELLELARQVRCPVLAIHGSYDPHPAEGVRAPLAAAIADFRFVLLEECGHEPWYERR
                     ARARFFAILQDELREARRAAGETSL"
BASE COUNT         1137 a         2544 c         2443 g         1017 t
ORIGIN      
        1 gcccgcagcc gccggatgac gcgcgccagc tcgcgcgccg tcaacccgcc ccagtgcttg
       61 ctgccggtgc cgggcatcac ctgctcgttg agcacgtcga catccaggct gacgtgcagg
      121 gcatcggcgc ccgagcgcgc gatgtcgcag gcgatccgct cgaaagcggc atccagcccc
      181 tgctcgtcga tgtcgtcgac ggtcacggcc tggatcgccc cctcggcgat ccaggcgcgc
      241 tcgcccggat cgagcaggca gaggccgaag aggtaggcgt cctccgggcg gaccttcggc
      301 gcgggaccgg ccagcccggt cagcgcgcgc gggccgcggc cgaggctggc gcccaggatc
      361 atgccgtgga tgcggccgga ggggctgacc tcgggccagt tgaggtcggc gtgggcgtcg
      421 atccagagca cggccagccg ctcggcggcg cgcgccgccc cggcgatgct ccccagcgag
      481 agcgcgtggt cgccgccgag cgccagcggc agctcgccgc gcgcgatcgc cgccgcgaca
      541 cgctcggcca gcttctcggc ggccgcgacg atctcgccct ggtagagcgc cccgcccggc
      601 tggagcccgg gcgtgccgag cgccagcggc gccaccggga taacctcgct ggggcgcagc
      661 cgggcaagca gcgcgtgact gcgggcgtcc tcgccggccc agcgcgcgcg cagggcggca
      721 gcgagcgccg ccgcgccctg ctccaccccg gcgcgcgccg cgccgaggtt cagcggcacc
      781 tggatcggtt gtatgggcgc cgcaaccagc cggttgagcg gctgccgggc ggccatcaca
      841 tgatctccgg cgccgcgatc cccaacaggt tgaggccgtt ggccagggtc tggcgcgcgg
      901 ccgccaccag cgcgatgcgc gcctgccgtt gcggctcggg cgcgctcagg acggggcagg
      961 cgcggtagaa gtcgttgaag cgcttggcga gctcgtagac gtagttggcg atcaggagcg
     1021 gcttgtattc ggacgcggca cgctcgacct cgctcgggaa gagcgcgatc tcctcgatca
     1081 gattcgtctc ctccggcgtc ggctcgccga agatcagctc gccctccggc agccgctcga
     1141 ccttctccag gatgcggcag gcgcgggcgt gggcgtactg gatgtagggc gcggcgtgcc
     1201 cctcgaagga gagcgcctcg tcgatgtcga agacgatcac gcgattgttg tcgcgtgcca
     1261 gcatgccgta cttcatgctg ccgatgccga catcgtgggc gatgcgcgcc ttcagctccg
     1321 gcgccagctc gggattcttc tcgtcgatga tctcgcgcgc gcgcgtcagc accgcctcgg
     1381 ccacgtcatc gtagaggatc gtgttgcccg agcggctgct catggcgccg ctcggcagca
     1441 tgacggtctc gtagctgaga tggaagcact tctcggcctg ctcgaagccc cacagctcca
     1501 tgatcttgaa gatctgctgg aagtagaggc tctgacgcac gtcgaccacc cagatcgagc
     1561 ggtcgatgcc gtactgctcg aacttctcct tggtcagcgc caggtccttg gtcgagtaga
     1621 gcgtggtgcc gtcggagcgc aggatcggca gcgtgcggta ggtctccttc tccagcccca
     1681 gccgctcgtc gatcttgacc accggcagac cgtcgctgat ctcggcgatg ccgcgctcca
     1741 gcagctcgcg cacgatctcg cgcccgcgct cctccacctg actctcgtaa aaccagacgt
     1801 cgaagtggac gcccagttgc tcgtagatgc gctggaagtc ctccatgctc caggctttgg
     1861 tctcctccca gagcgccacg aacgccggat ctttctcctc ccagcgctgg aaggtctcgc
     1921 gcacctcttg ctgccactcc ggcacatgcg cccaccagtc gaggtgggtg gccaggcgct
     1981 cccactgctc cagccgcgcc cgcccggcga cgaccgcctc gcgcgccgcc gcctcggcct
     2041 cctccgactc cggcggcgcc agcaccgcgc tctcggcgtc ggccagctcc tgcgccagct
     2101 ctttgccggc gatgggccag aactggggga tcgtctcgtt gtcgtagaag gcgtcgggct
     2161 tgagctcgcg ctggtgcgcg agctggccca gcaagtaggc gacatcctcg ccgatcttat
     2221 gcgtcttcca cagctccttc atcatgcgat cgatgcgctc gacgaaggcg agatcctcgc
     2281 gcgagagcat gttgaggaag gcggccagat cctggcggta gttcaggcgc tgcacggcct
     2341 cggtgtagac ctggcccagc cagcggccgc gctcgaccgg gggcggctcc tcgccctggt
     2401 ggaagcgccg gtagcaccag agcgtctgga tcacgtgcat gccgatgtcg ccgatgtagt
     2461 tggcctctaa gaggtcgaag ccggcgaagc gcaggatgcg gctgagcgcg ctgccgaggc
     2521 aggcgttgcg caggtggccg atgtggaagg ccttgtgcgt gttgggctgg gagaactcga
     2581 ccatgacgcg gtcggtctgc gccgggccgc gtccgtagtc ggccccggcg tccacgaccg
     2641 agcgcaccac gacgttgctg aagtggttgg tatcgaagat gacgttgatg tagccgttga
     2701 gcgcctccac cgcggcgaag ccgtcctgcg ccagccgccc ggctacctgc ccggcgatct
     2761 cctgcgcgcg cgcgctgacc agctccttga cgcgccgctt ggcctccttc ttggagagcc
     2821 ccgccagttc ctcggcggac tcggccatga gcaggtcgtt ggcgatctga tagctgaccg
     2881 acgaagacgt cccccagaca ccggagaagg ggatggcgcg catctcgaag cggcgccggc
     2941 catagccgag cgtctccagc gcgtcgttga tcagggccgc ggcccgttgc tcttcgttgt
     3001 ggaacatcgt ttccctgcct agcgcggctg agttcgtgag ctccgacgga ggtgtccgga
     3061 ggcgacgccg ggcacgccac ggaccggcgc caggcgccgg tgcggctcca agtataccgg
     3121 cggcgcgcta cttcttgccg gtgacggtct tgatcatggc gcggacaccg gccaaagtgg
     3181 agtaggcgga gatgatgggg gaggcgacct cctcgccgac gaactcggcg gtgccgcgga
     3241 cccgcgaggc ggccagggtg agctgctcca gcagcgggat caccttgtcc ttgagcagca
     3301 gcacgagcca gaggacggcg ccgaccagcg cgcccaagag caggatgacc aggatcatcg
     3361 agagcgccat gaagatgatc gagatctcgg tgatcttctg cagcgccgac tcgcttgagc
     3421 tacccagcag gtagaggacg acaaacagca cgacgaagag gacgatcagg ccgatcacga
     3481 tcagatagag ccagcccttg cgcacgctgc tcccctccta accctcagtc tcagcgagct
     3541 ccgcgcggat ccgtcgctgc ctggcgcgcg tccgccgcgc gcgccacccg tcgcacccgc
     3601 cgccggggac gcggctcgtg cggcgcggtg aagaaccaac agatgacggc gccgagcagg
     3661 ctgcccgcga gcgccgccgg cagcgggatg ctccccaggc gaaagaattc caccccggcg
     3721 agaatcgcaa caatctcgcc agcgagaaac ccgccaactc ctgccagcca gaagcagggg
     3781 agctgccaca gccgccgccc gagcagcgcg tgcgccagca ggccggcgat gctgccgagt
     3841 gtcagcgcga ggatcagtgt gggagacatc gcttcccttc cgccctggac gcctccagcg
     3901 tgtccggacg gatcttagca gaaacgcgcc gcgccggacg acgccggcgc ggattgcctg
     3961 caggttgccc gcaggttgcc cgcaggttgc gctccacctt gccctcgaac tcaacccgcc
     4021 gtggctctcg tcccgccctc aggagagggc gacgcgctgg gcgcctggcg gctagtcgcc
     4081 gccgccgcgt gcagctcgaa gcccgggtgc agttgagcgc gcagcgcgtc tagctcggcg
     4141 tcgaagtcgg cgccggtcgg gccggagagc accagatgca gcgccgcccg ggtcgccgtc
     4201 tccagcgccg gccgcgccag cgtctcccgc cgcaggatca gcccgtggta gaccagcagc
     4261 gcgaccgcga tcacggccac gccgagcgcg gcgctgatcg cggagacgaa cccgcccgcg
     4321 ggcgtgacgt tcagcagcag gttgaacagc cggtagagaa tcaccgagaa gctggccagc
     4381 gtcgcgatca gcgacccgcc cagcaccgcg tagagataga accgccggct ggtcgtccag
     4441 cgctcccgct cggggttcgc ctggcggcgc gcctggatcg cgtaccagtg ccagagccac
     4501 acggccccgc ccaccagcgc catcgcgccg tactggctca cctggccgcg ccaccaggcg
     4561 gcgctgaagc cgaccgtgcg gctgccgccg agcaccagat ccaccaggat gccggccagg
     4621 taggcggcgc cgagcgcggt gaaggccagc cccaccagcg acaggccgta gcggtagaag
     4681 cggctgaccg agtcctgaaa cgccgcgctg ccgaagcggc gcgcttcatc ccgaatccgc
     4741 tgccggtaga agagccagaa cgcgacgaac gggatggccc cgacgggcgg ctcgatcagg
     4801 cgccgcagga atgcgcgcgc gccggaggac accggcacgc tcagtagcgc ccgcaagacg
     4861 gtctccaggc tggagctgag cgccagcagc gtcagtaccg aggtgaccag gatgacgccg
     4921 tacagataga tgcggcgcag ggtcgactgg cgctcgctga gcgagaacca gtcaccacgc
     4981 tccagcacgg actgcgtgta ccaccagtgc agggaccaga ccgccagccc ggccaccacg
     5041 gacgccgccc cgtcggccag tcgcggcagc caccaacgct cgccggcgaa gatgacgcct
     5101 tgtgtcccga agaccgcgtc gccgatcaga tcgaggagct ggaccgcgcc gacgatcagc
     5161 agggccgcgc cggccgacag cgcgatgtag cggtacaggc gcgggtgcca ggtagcatcc
     5221 atggctatcg tgccccggcg aaggtcgcgc aggcgcatcc tgacgtggta gatccagacc
     5281 aggctggtca cgatcaccgc ggcgagcgcg cccggaaccc cgccgctgaa cgtctcgcgg
     5341 gcatggccga tctccagtac ggcgaagcgg acgaggtcca ccgccggact gacccacatc
     5401 cagaacgaga ccagcagcac cgccgcgaag aagaacgtcc gcacgaccga ctggcgctcg
     5461 gcctcgccgg tctccccctg cgccacggca cgctcggcca gccaccagtg cagcagccag
     5521 atcggcaggg ccacgaccac cagggccgcg tacatgctga cctcgcggcg cagatcgccg
     5581 ggtgtgcggc cgataacctc gcgccagcca agcgcctcgg cgaactgctc cagcgccagc
     5641 cgcagcaggt tcatgagccc gaacgcccag acggccaggc tcaccgccgc gatgaagtag
     5701 acgtagatgc gtcgcgcgct ctgcatcgcc ctaccctctc cctctctccg aggccaggtc
     5761 ggctgacggt gtagccgtcg gggcggccgg tggtggcaag gcgtagatca cctcgatgcc
     5821 ggccagcggc tcatcgatct tccagccgcc cgcttcctcg accagccgaa tgctgcgttg
     5881 ctcggtgtag cggctcacgt cgagcccgga ccccgacgcg cgctcgacgg ccaaatacag
     5941 cgtcacgaca ccattggtgc gattgacgtg gtcgatgcgg accaccgtgc tttgctcaag
     6001 cgggggttgg aacttggtgg cgtaccagac gtattgatcg tagctgagct ggtcgcgcac
     6061 gcgctggctg aacgatgcgt aggccgcctg catatcgccg gtctggaacg cctggtagta
     6121 gcgctggaag gcgtattcgg gcgagccggc cgggtagctg gcgggcttgc ccgcgtcgag
     6181 cgcgagagtc gccacgacgg ctgcgatgac cagcagcagc accaggccgc cgatgatggc
     6241 gatggttctc cccgcgtggc cggtcgcggt ctggctggtc atctagttgc ctccccggag
     6301 tcgcgcggaa cgagggatcg ctggggaagc gatctatctt cagcctagcg gcgatctatc
     6361 acaaccgcat atctcgcccg cggccagcgt gacagtttcg tgtcaggtca aagcgacgtc
     6421 tcaccggctg ctcgccgcgc ctctcgcaac tcgtcctgaa ggatggcgaa gaatcgcgcc
     6481 cgcgcccggc gctcgtacca cggttcgtgg ccgcactcct ccaggagcac gaagcggaag
     6541 tccgcgatgg cggcggcgag cggcgcgcgc accccttcgg cggggtgtgg gtcgtagctg
     6601 ccgtggatcg ccagcaccgg gcagcgcacc tgccgcgcca gctccagcag ctcgccggag
     6661 gcgcgcagcg cctccgcttc cccccagacc tgctggaata cgtcgaagcg cacctcgagc
     6721 aactcatcgt cgtggggcag cggatcgtag ctgtcggccc tggcgaagat cgcgcccagc
     6781 cggcgcagca cgtcgtcccg gcccgcgccg gctgggccat tgagggagat cagcagcgcg
     6841 tcgatctccc gccgctccgc cgcgctcagg cgctcgagcc gcgtctgctc gataccttgg
     6901 gcgtagcgcg catcgtagac accgctgccg acgaggatga gccgggcgac gagctcggga
     6961 tgacgcgccg ccaggatcaa actcagcatc gcgccccagg aggagcccac caacgtcacg
     7021 ggcaggcgcg cgtgggcgag gagaagcgca cgcagctcgg ccacctgacc gtccaggctg
     7081 gcctcggtct gcaggggttc gagcaccccc cactcggcgc ccagctcgcg cgcgaccggc
     7141 g
//
LOCUS       DASUBI010000564         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_892978, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000564 DASUBI010000000
VERSION     DASUBI010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892978"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1330)
                     /gene="moaA"
                     /locus_tag="VFU72_10880"
     CDS             complement(281..1330)
                     /gene="moaA"
                     /locus_tag="VFU72_10880"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU5424036.1"
                     /translation="MVVEPVAIKFSESGLLDRATGQPLTRDAFGRAMTYLRISLTDRC
                     NLRCVYCMPAIGMKFAPRQELLTDDEILAVVRAAAKIGFSKIRLTGGEPTVRPHVADI
                     VREIAHTPGIDDVAMTTNGLLLGRLAGDLKAAGLRRVNVSIDSLQSERFTRITRGGKI
                     EKVWEGIAAAEAAGLTPIKLNAVVVRGQNDDEVADLAALTLTRPWQMRYIEVMPLEGV
                     GEVHDEGLVTTAETIELIERAHGELEALEAPLGDPARIYRLPGGVGTIGFISPVSQPF
                     CAFCNRIRLTADGKLRLCLLRNDEVDVRDIRRGGGSEEELIERIRLGVWRKPWGHGLA
                     EGDRDTGRGMSQIGG"
     gene            complement(1520..2215)
                     /locus_tag="VFU72_10885"
     CDS             complement(1520..2215)
                     /locus_tag="VFU72_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5424037.1"
                     /translation="MENQELQRVMIVVAHPDDMEFSSAGTLAKMAKEGKRVRLVQCTS
                     GDKGTSRRDINSQELAELREGEEREACRRLGVQEVEFLRLKDGELMPDLNFREKVVQQ
                     IRDFRPDVLITHDPFRPYSLHPDHRAVGITAVDSVYPTARDPLYFPEHLQSGLEPHKV
                     AELWLFGSEYPDTYIDISDTFEDKINALKAHKSQVGEAETLADRLRERAAEVGKAQNI
                     PLAEAFKVIKMRR"
     gene            2389..3642
                     /locus_tag="VFU72_10890"
     CDS             2389..3642
                     /locus_tag="VFU72_10890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5424038.1"
                     /translation="MIDRAARSLKVLMIAPTSFFADYGCHVRILEEINALGARGHRVT
                     LATYHNGNDVPGLDIRRSVDVPWRKRVIVGSSRHKLYLDAVLSATCVRAGYSLRPDLI
                     HAHLHEGALIGAGLARVLRRPLLFDYQGSLTEEMLDHRFLRPGGPLPPVVRRLERLID
                     RLPDLIITSSINAEQRLRERGYRGRMQPVLDAVDTNRFAPDTLAPDEKRALRRSLGIP
                     DDGPIVVYLGLLAPYQGTDLLLETARSVLDQIPNATFLVMGFPGVERYQALAGQLGIA
                     RHVRFPGRIPYREAQRYLALGDVAVAPKMSLTEGNGKLYNYMAMGLPVVAFEAAANRE
                     ILGDFAAYAPPGDAAAFASQVTSLLSRPALASELGAAVRAEAIRHHSWQARVDDLLAA
                     YARVLPAHQRALGGTSSALSGQSTD"
BASE COUNT          594 a         1362 c         1251 g          637 t
ORIGIN      
        1 tgccccccac gttccccttc ccccgttgca gaggcgcggg ggcagcaagg caagggtgga
       61 cacggagatc tgcccctaca ggcggggata cacagcggcc agcgagccgg caaaccgctc
      121 taaagagcta ccgggcggat cgcggccagg cagcgacagt atcgccgctg ccgtaacctg
      181 cgcaagaccg agagacaagg agtgaggcgc ccactatgtc gcgagcgcct cactcccgga
      241 atgccgaagg agatacgaac tgagttggac ggggcggagg ctagccgccg atctggctca
      301 tgccgcggcc ggtgtcgcgg tcgccctcgg ccaggccgtg gccccagggc ttgcgccaga
      361 cgccgagccg gatgcgctcg atcagctcct cctcgctgcc gccgccgcga cggatgtcgc
      421 gcacgtcgac ctcgtcgttg cgcagcaggc agaggcgcag cttcccgtcg gcggtcagac
      481 ggatgcggtt gcagaaggcg cagaacggct ggctgaccgg gctgatgaag ccgatcgtgc
      541 ccacgccccc gggcagccgg tagatgcgcg cggggtcgcc cagcggcgcc tccagcgcct
      601 ccagctcgcc gtgggcgcgc tcgatcagct cgatcgtctc ggcggtggta accagaccct
      661 cgtcgtggac ctcgccgaca ccctccagcg gcatgacctc gatatagcgc atctgccagg
      721 gtcgggtcag ggtcagcgcc gccagatcgg ccacctcgtc gtcgttctgc ccgcgcacga
      781 cgaccgcgtt gagcttgatc ggggtcaggc cggccgcctc ggccgccgcg atcccctccc
      841 agaccttctc gatcttgcca ccgcgcgtga tccgcgtgaa gcgctcggac tggagactgt
      901 cgatgctgac gttgacccgc cgcagaccgg ccgccttgag atccccagcc aaccgcccca
      961 acagcagacc attggtggtc atggcgacgt cgtcgatgcc gggggtgtgc gcgatctcgc
     1021 gcacgatatc ggccacgtgc gggcgcacgg tcggctcgcc gccggtcagg cgaatcttgc
     1081 tgaaaccgat cttggcggcc gcgcgcacca cggccagaat ctcatcgtcg gtgagcagct
     1141 cctgccgtgg ggcgaacttc atcccgatgg ccggcatgca gtagacgcag cgcaggttgc
     1201 agcgatcggt gagcgagatg cgcaggtagg tcatggcgcg cccgaacgca tcgcgtgtca
     1261 gcggctggcc cgtcgcccgg tccagcaacc ctgactcgga gaacttgatc gcgaccggct
     1321 cgaccaccat gactgcctca cctcatctag cgctccacgg cccgtgggca cccccctacc
     1381 ttagcatccg gccgggagat cggcaacacg aaagttgact aattcggggc gcgatgagcc
     1441 ggacgacccc tcctccgacc tgtcgaatac cccacgtcga gacgggatcg ttcgtcctca
     1501 gcctcacccg ccggtcgggc tagcggcgca tcttgatgac tttgaacgcc tcggccagcg
     1561 gaatgttctg ggctttgccg acctcggccg cgcgctcacg cagccggtcc gccagtgtct
     1621 ccgcctcgcc cacctggctc ttgtgcgcct tgagcgcgtt gatcttgtcc tcgaaggtat
     1681 ccgagatgtc gatgtaggtg tcggggtatt ccgagccgaa gagccacagc tccgccacct
     1741 tgtgtggctc caaccccgac tgcaggtgct cggggaagta gagcggatcg cgcgccgtcg
     1801 gatagaccga gtcgaccgcg gtgatgccga ccgcgcggtg gtcggggtgc aacgagtagg
     1861 gccggaaagg atcgtgcgtg atcaggacgt ccggccggaa gtcgcgaatc tgctgcacga
     1921 ccttctcacg gaagttgagg tcaggcatca actcgccgtc cttcaggcgc aggaactcga
     1981 cctcttgtac gcccagccgc cggcacgcct cgcgctcttc tccctcgcgc agctcggcca
     2041 actcctgcga gttgatgtcg cgccgggagg ttcccttatc gccggaggta cactgcacca
     2101 gccggacccg cttcccctcc ttggccatct tggccagcgt gcccgccgac gagaattcca
     2161 tgtcgtcggg gtgggccacc acgatcatga cacgctgtag ctcttggttc tccattgtct
     2221 gaaccactgc tccctttcca gcgcccatgc gcactctcgc ctggactatt gacgatgcgc
     2281 cgccgttcgt caagacggca acccgcggca gggccgctcg ttctgttgcg ggagggcatg
     2341 ctacccttgc cggctggcgg gtagttgtcc agggaggggc acgccagggt gatcgatcga
     2401 gccgcgcgat cgctgaaggt cttgatgatc gcgccgacct cgtttttcgc ggactacggc
     2461 tgtcacgtgc gcattctgga agagatcaac gcgctcgggg cgcgcgggca ccgcgtcacc
     2521 ctcgcgacct accataatgg aaacgacgtg ccagggctgg acatccggcg ctcggtcgac
     2581 gtgccctggc gcaagcgggt gatcgtcgga tcctcacgcc acaagctcta tctcgacgcg
     2641 gtcctctcgg ccacctgcgt gcgcgccggg tactccctgc ggcccgacct gatccacgcg
     2701 cacctgcacg agggcgcgct gatcggcgcc gggctggccc gcgtgctgcg ccggccgctc
     2761 ctcttcgatt atcaaggcag cctcaccgag gagatgctcg accatcggtt cctgcggccg
     2821 ggcgggccgc tgccgccggt cgtgcgccgc cttgagcgcc tgatcgaccg cctgcccgac
     2881 ctgatcatca ccagcagcat caatgccgag caacgcctgc gcgagcgcgg ctaccgggga
     2941 cgcatgcagc cggtgctcga cgcggtggac accaaccgct tcgcgccgga cacgttggcg
     3001 cccgacgaaa agcgggccct tcgccgctcg ctcggcattc ccgacgacgg ccccatagtc
     3061 gtctaccttg gcctgctggc gccatatcag ggcaccgatc tgctgctgga gaccgcgcgc
     3121 tcggtgctgg accagatccc gaacgcgacc ttcctggtga tgggcttccc aggggtcgag
     3181 cgctatcaag cgctggccgg gcagctcggc atcgccaggc acgtccgctt ccccgggcgc
     3241 atcccctatc gcgaggcgca gcgctatctg gcgctgggcg acgtcgcggt cgcgcccaag
     3301 atgtcgctga cggaaggcaa cggcaagctc tacaactaca tggcgatggg gctgccggtg
     3361 gtggccttcg aggccgccgc caaccgcgag atcctgggcg acttcgccgc ctacgccccg
     3421 cccggagacg ccgcggcctt cgcgagccag gtgaccagcc tcctctcgcg cccagcgctg
     3481 gccagtgagc tgggcgccgc tgtgcgcgcc gaggctatcc gccaccactc ctggcaggcg
     3541 cgcgtcgacg atctgctggc cgcctacgcg agggtgttgc ccgcccacca gcgggcgctg
     3601 ggcggcacgt cttccgcgct gtcgggccaa tcgacggatt gagtcggtcg ttggtgcccg
     3661 tcgacacgct cgggtggcat cgaggacgga tccttcgacc gcgctcagag cctgccctga
     3721 gcctgccgaa gggatgacag ggttagtacg gagcggacga cgaaggcttg ccccgcccgg
     3781 ttgggcatcc cctttaccct gtcatcctga cgcgcagtcg aaggatccgt cctgccctac
     3841 tgct
//
LOCUS       DASUBI010000565         8273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_389100, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000565 DASUBI010000000
VERSION     DASUBI010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389100"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="acnA"
                     /locus_tag="VFU72_10895"
     CDS             <1..1671
                     /gene="acnA"
                     /locus_tag="VFU72_10895"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5424039.1"
                     /translation="AGPRRPQDRVSLGDVGRSFHAAFPESFSAPPPRSPAAVEERFGR
                     EGGTADAALFREHASATLVSRHTPVEIDLDDRRAVIGDGSVVIAAITSCTNTSNPAVM
                     VAAGILAKKAVERGLQVSPAVKTSLAPGSGVVTAYLDRAGLTPYLEALRFHLVGYGCT
                     TCIGNSGPLPEPVTEAIQKNEYVGVAVLSGNRNFEGRIHPLARASYLASPPLVVAFAL
                     AGTIEIDMTLEPLGYDPNGEPVYLKDIWPTTQEIQEAIRTALGPDLFAERYADVFHGD
                     DTWRALPIPEGDLYNWNGDSTYIQEPPFFQGLGPEPGPLLNIEGARVLAVLGDSVTTD
                     HISPAGSIPPQSPAGRYLVLNDVPQREFNSYGARRGNHEVMVRGTFANIGLRNALAQG
                     REGGYTTYLPEEELTSIFEASVKYQEAGTPLLVIAGKEYGSGSSRDWAAKGTLLLGVK
                     AVLAESFERIHRSNLVGMGVLPLQFKAGENAASLGLTGREVYDIGGIAEGLTPRKVVT
                     VHAKDEQGNEKTFEAIVRIDSPMEIEYYRHGGILQMVLRRLAAQEAAD"
     gene            1915..2460
                     /locus_tag="VFU72_10900"
     CDS             1915..2460
                     /locus_tag="VFU72_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5424040.1"
                     /translation="MPKLCRVRPLTDDEAATLERLARSRTAAARLVERARSLWRSSQG
                     WRVPAIATAAGRSPETVREWIARFNAQGLDGLADAPRTGRPPTYTAEEIGTLIATALS
                     DPQTLGQPFGSWTCQRLATYLAEEQGIAIKRSRVQELLVREGLRWRQQERWFGATVDP
                     AFAEKRGPSRSSTPRPRRTAS"
     gene            2457..3041
                     /locus_tag="VFU72_10905"
     CDS             2457..3041
                     /locus_tag="VFU72_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5424041.1"
                     /translation="MICLDELGPESAKSYPGAQLIRPEPTATQPARRATQEIDYGRRG
                     AGYIFGAFQPATGAAFTAPYDRRTTAHFVDFLEQVDGWLPTAPDRVYAILDNLQMHRA
                     ADVLLFALAHPRWEFVFQPVYAAYLNLIEPWWKVLRSLALKGRRFETWDELAQAVAAA
                     TAYWNDHRHPFHWGHRRRHRPARKPGIALAANVR"
     gene            3146..4315
                     /locus_tag="VFU72_10910"
     CDS             3146..4315
                     /locus_tag="VFU72_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU5424042.1"
                     /translation="MARIIDVRVRTAPLRSDIRNAFIDFSQMTVAVVAVITDAVRDGR
                     RVIGYGFNSNGRYAPSGLLRDRFVPRLLAAEPDDLVSADFPELDPHKAWQVLLRGEKP
                     GGHGERSVAVGTLDMALWDAVAKLEGKPLYRLLAERYHGGQADERVFVYAAGGYYQPG
                     KNLRALREEMRRYRDLGYTLLKLKIGGASLDEDQRRIEAVLEVAGAGENVAVDANGRF
                     DLPTALAYAEALAPYGLRWYEEPGDPLDFALHAEVAARYPAPLATGENLFSLPDARNL
                     IRYGGLRPERDVLQFDCALSYGLVEYLRILDMLEEHGWSRRALIPHGGHQMSLNLAAG
                     LGLGGNESYPGVFQPFGGFADAIPVENGYVRLPDIPGVGFEAKRELYDLLRSVAV"
     gene            4389..4655
                     /locus_tag="VFU72_10915"
     CDS             4389..4655
                     /locus_tag="VFU72_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5424043.1"
                     /translation="MEHGKHYNVTLGERGRLVLPADLRRQLGLKPGDRLIITSDDEGG
                     FRVVSAREVARRFFGIYRDLAPGRSLVDELIAERREESRREDAE"
     gene            4652..5068
                     /locus_tag="VFU72_10920"
     CDS             4652..5068
                     /locus_tag="VFU72_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5424044.1"
                     /translation="MTSRAPNALLDASALLAYLRREPGGEVVLNVLTAGASITTINYA
                     EVLARLSDAGEDPTSAHHRFQAQGLIGGPVTIVAMTLDDAVTIARLHGATRSQGLSLA
                     DRACLAVGLRLGLPVLTADRKWAEVDVGVMVRLMRT"
     gene            complement(5108..6373)
                     /locus_tag="VFU72_10925"
     CDS             complement(5108..6373)
                     /locus_tag="VFU72_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424045.1"
                     /translation="MTSGRRLVPLTIMISLLAWATAQASFAVSLWKPRLWPAGVALVV
                     LGGITPLIYAVNLRVLPLFSGRNWRSPRLLLLTLVFAQSAWLVFLGRAGLGGTVEAVG
                     AAGCLSAALVFAYATGGPVRTPPAPGAAARVSQKHVHGDTTGRRFVKLAGVYLLVGLG
                     LGLALCFWRPGGGRWELVWAHALLVGWFLSMVSGVCYHVLPRWSRRGWRRPWLLRLHF
                     LLIAFGLPVMLLALALDAAPLFAVAGPLEAAALVIFVWNILPLALDLPRLTRFGLVAA
                     GLLLALGVSLGALMAVAPAWQPRLHQTHAELNLFGWAGLLVSGFGYYLFPRLAGRPLR
                     HPRLAALQLGALLAGVLLSASAWYAYVEGIVAARWPIVAGSALAAAGLLGFGLLMGST
                     FLDGRARLAGRHQAATVGGRQTVGDATRT"
     gene            complement(6370..6594)
                     /locus_tag="VFU72_10930"
     CDS             complement(6370..6594)
                     /locus_tag="VFU72_10930"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEU5424046.1"
                     /translation="MTGLTTEGKRQIIETRLIRELVEDYPNLMPVLASYGFDLCCGGE
                     HTLAEAAELHGLDPEQVVREVTAAFGELAA"
     gene            complement(6654..7754)
                     /gene="nirK"
                     /locus_tag="VFU72_10935"
     CDS             complement(6654..7754)
                     /gene="nirK"
                     /locus_tag="VFU72_10935"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEU5424047.1"
                     /translation="MNLMRRGLMRGLALIALVILSLSVASVGAWTTGGGGTQAAMNMP
                     STPDAATLAAEVAGLTQLPQPVVAQPVGHRDPTTVKVTLVAKEVKALLDNGVGYTFYT
                     FNGTVPGPMIRVREGDTVELTLKNDPTSTSPHDIDLHAVTGPGGGAAVTSVKPGSEAT
                     FTFKALHAGTYVYHCAAAPAPMHIANGMYGLIVVEPPEGLPPVDHEFYVMQGDFYPAG
                     NVGDKGMRTFDMTKMMAEDPEYVVFNGSVGSLTGEHELESKVGDTVRIFFGVGGPNLP
                     SSFHIIGESFDTVSVEGGTLENHNVQTTMVPPGDATMVELTTDYPGNYSLVDHSLGRV
                     MKGAIGTLHVDGQADPSIFNVIKAGDAGSAGH"
     gene            complement(7793..>8273)
                     /locus_tag="VFU72_10940"
     CDS             complement(7793..>8273)
                     /locus_tag="VFU72_10940"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEU5424048.1"
                     /translation="DWMSGGAALRDYWDQLTHTQGAATHPLADGWSRGALHFLLDHGA
                     YHWLAPVAAVVETLVGLALILGVFTALAAFLGVLLSVSHMLAGTSSLDPVLLVLAIGL
                     MLAWKTAGWLGFDRWLLPMVGMPWQGGALITRLPRSTSRLHLPAQLVRRPANDDSRT"
BASE COUNT         1371 a         2920 c         2764 g         1218 t
ORIGIN      
        1 gccggaccgc gccgcccgca agaccgcgta tcgctgggcg acgtcggccg cagcttccac
       61 gcggcctttc ccgagagctt ctccgcgccg ccgccgcgct cgccggcggc cgtcgaagag
      121 cgcttcggcc gcgagggcgg caccgccgac gcggccctct tccgcgagca cgcctcggcc
      181 acgctggtct cgcgtcacac cccggtcgag atcgatttgg acgaccgccg cgcggtcatc
      241 ggcgacggct cggtcgtgat cgccgccatc accagttgca ccaacacctc caaccccgcc
      301 gtgatggtgg ccgccggaat cctggccaag aaggcggtcg agcgcgggct gcaggtcagt
      361 cccgcggtca agaccagcct ggcgcccggc tccggcgtgg tcaccgccta cctcgaccgc
      421 gccggcctga ccccgtatct cgaagcgcta cgcttccacc tggtgggtta cggctgcacc
      481 acctgcatcg gcaacagtgg gccgctgccc gagccggtca ccgaagccat ccagaagaac
      541 gagtacgtcg gcgtggccgt tctcagcggc aaccgcaatt tcgagggccg cattcacccc
      601 ctggcgcgcg cctcctacct ggcctcgccg ccgctggtgg tggccttcgc cctggccgga
      661 accatcgaga tcgacatgac gctcgagccg ctgggatacg accccaacgg cgagccggtc
      721 tacctgaagg acatctggcc cacgacccag gagatccagg aggcgatccg cacggcgctc
      781 ggcccggatc tcttcgccga gcgctacgcc gatgtcttcc acggcgacga cacctggcgc
      841 gcgctgccca tccccgaggg cgacctctac aactggaacg gcgattcgac ctacatccag
      901 gagccgccct tcttccaggg cctcggcccc gagccgggcc cactgctgaa catcgagggc
      961 gcgcgcgtgc tggcggtgct cggcgactcg gtcacgaccg accacatctc acccgccggc
     1021 tcgatcccgc cgcagagccc cgccgggcgc tacctggtcc tcaacgacgt gccccagcgt
     1081 gagttcaaca gctacggggc gcgccgcggc aaccacgagg tgatggtgcg cggcaccttc
     1141 gccaacatcg ggctgcgcaa cgccctggca caggggcgcg agggcggcta caccacctat
     1201 ctgcccgagg aggagctgac ctcgatcttc gaggcctcgg tcaagtacca ggaggcgggc
     1261 acgccgctgc tggtcatcgc cggcaaggag tacggcagcg gctcctcgcg cgactgggcg
     1321 gccaagggaa ccctgctgct gggcgtcaag gccgtgctgg ccgagagctt cgagcgcatc
     1381 caccgcagca acctggtcgg catgggcgtg ctcccgttgc agttcaaggc aggcgagaac
     1441 gccgcctcgc tcggcctgac cgggcgcgag gtctacgaca tcggcggcat cgccgagggg
     1501 ctgacgccgc gcaaggtggt cacggtccac gccaaggacg aacaggggaa cgagaagacc
     1561 ttcgaggcga tcgtgcgcat cgacagtccg atggagatcg agtactaccg ccacggcggc
     1621 atcctgcaga tggtcctgcg ccgcctggcg gcgcaggagg cggccgacta gcacggggag
     1681 cggtgcgcca gcgcgcgacc cttgacaagg ctgcgtgcca agagaggacg gatccttcga
     1741 ctgcgctcag agcctgccct gagcctgccg aagggatgac agggtaagtg gtgtggcgta
     1801 cgacgacgat gatcgtattc gtcagcccct atctcacccg gtcatcctga gcttgtcgaa
     1861 tggtgcaacc ggcaagtcct gctagactag cggtcagcga gggagggcag gacgatgccg
     1921 aaattgtgcc gggtgcggcc gttgacggac gacgaagcag cgacgctgga gcggctggca
     1981 cgcagccgca ccgccgccgc acgcctggtc gagcgggcgc gcagtctgtg gcgctcgtcg
     2041 caaggctggc gcgtcccggc catcgcgact gcggccgggc gcagcccgga gacggtgcgg
     2101 gaatggatcg cgcgcttcaa cgcccagggg ctggacgggc tggccgatgc cccccgcacg
     2161 ggccgtccgc ccacctacac cgccgaagag atcggcaccc tgatcgccac cgccttaagc
     2221 gatccccaga cgctggggca gcccttcggc agttggactt gccagcgcct ggcgacctat
     2281 ctggcggagg agcaggggat cgccatcaag cgcagtcgcg tccaggagct cctggtgcgg
     2341 gagggcttgc gctggcgcca gcaggagcgc tggttcgggg cgacggtcga tccggccttc
     2401 gcggaaaaaa gggggccatc gcgcagctct acaccgcgcc cccggcggac agcctcgtga
     2461 tctgtctgga cgaactgggg cccgagagcg ccaagagcta tcccggagcg cagctcatcc
     2521 gaccggagcc gaccgcgacc cagccggcca ggcgcgccac ccaggagatc gattatgggc
     2581 ggcggggggc cggctatatc ttcggcgcct tccaacccgc cacgggagcc gccttcaccg
     2641 ccccctatga ccggcgcacc accgcccact tcgtcgactt cctggagcag gtcgatggct
     2701 ggttgcccac cgcccccgac cgcgtctacg ccatcctgga taatttgcag atgcatcgtg
     2761 cggccgatgt gctgctcttt gccctggccc atccgcgctg ggagttcgtc ttccaaccgg
     2821 tctacgccgc ctacctcaac ctgatcgagc cctggtggaa ggtgctgcgc tcactggcgc
     2881 tcaaagggcg gcgcttcgag acctgggacg agctggcgca ggcggtcgcc gccgcgacgg
     2941 cctattggaa tgaccatcgt caccccttcc actgggggca ccggcggcgg catcgtccgg
     3001 cgcgcaagcc gggcatcgcg ctggctgcta atgtgcgcta gtgttgccgg ttgcaccacg
     3061 aaggatccgt ctttaatgcc tgccatacgc ctcgaaggcc acaagtctct ccaatccgtt
     3121 cgtgacggtt gcagcaaggg gcaacatggc cagaatcatc gacgtgcggg tgcggaccgc
     3181 gccgctgcgc tcggacatcc gcaacgcctt catcgacttc agccagatga ccgtggcggt
     3241 ggtggccgtc atcaccgatg ccgtgcgcga cggccggcgt gtcatcggtt acggcttcaa
     3301 ctccaacggg cgctacgccc ccagcggcct gctgcgcgac cgcttcgtgc cgcgcctgct
     3361 ggccgccgaa cccgatgacc tggtcagcgc cgacttcccc gagctggacc cacacaaagc
     3421 gtggcaggtg ctgctgcgcg gcgagaagcc gggcgggcac ggcgagcgct cggtggcggt
     3481 cggcacgctc gacatggcgc tctgggacgc cgtcgccaag ctcgagggca agccgctcta
     3541 ccgcctgctg gccgagcgct accacggcgg acaggccgac gagcgcgtct tcgtctacgc
     3601 cgccggtggc tactaccagc cgggcaagaa tctgcgcgcc ctgcgggagg agatgcgccg
     3661 ctaccgcgac ctcggctaca cgctgctcaa attgaagatc ggcggcgcgt cgctcgacga
     3721 ggaccagcga cgcatcgagg cggtgctgga ggtcgccggg gcgggcgaaa acgtggccgt
     3781 cgacgccaac ggccgtttcg atctgccgac cgcgctggcc tacgccgagg cgctcgcacc
     3841 ctacggcctg cgctggtatg aggagccggg cgacccgctc gacttcgcgc tgcatgccga
     3901 ggtggccgcg cgctaccccg ccccgctggc gaccggcgag aatctcttct cgctgccgga
     3961 cgcgcgcaat ctcattcgct acggcgggct gcgccccgag cgcgacgtgc tgcagttcga
     4021 ctgcgccctc agctacgggc tggtcgagta tctgcgcatc ctcgacatgc tggaggagca
     4081 cggctggtcg cggcgcgcgc tgatcccgca cggcggccat cagatgtcgc tcaacctggc
     4141 cgcggggctc ggcctgggcg gcaacgagtc gtaccccggc gtcttccagc ccttcggcgg
     4201 tttcgccgac gccatcccag tcgaaaacgg ctacgttcgc ctccccgaca tccccggcgt
     4261 cggcttcgag gccaagcgcg agctgtacga cctgctacgg agtgtcgccg tctaacagga
     4321 gtacgagcag gcgagctggg tgccgcgact gaacgtgggc acagttgaat tcgtggtacg
     4381 attcggacat ggagcacggc aagcactaca acgtgacact gggagaacgc ggacgcctgg
     4441 tgctgcccgc tgatctgcgc cggcaattgg gattgaagcc aggcgaccgg ctgatcatta
     4501 cctctgatga cgaaggcggc tttcgcgtcg tatcggcgcg cgaagtggca aggcgcttct
     4561 tcggcatcta tcgcgatctc gcgccggggc ggtcgctggt cgacgagttg atcgccgaac
     4621 ggcgcgaaga gtctcggcgc gaggacgctg agtgacgagc cgtgccccca acgcgctcct
     4681 cgacgcctct gccctgctcg cgtatctccg ccgcgagcca ggtggcgaag tcgtcttgaa
     4741 cgtcctcact gccggtgcat ccatcaccac catcaattac gccgaagtcc tcgctcggtt
     4801 gagtgacgcg ggtgaggatc caacatcggc gcatcaccgt tttcaagcgc aagggctcat
     4861 cggcggcccg gtgacaatcg tggctatgac cttggacgat gccgttacca tcgcgcggct
     4921 acacggggcg actcgctcac agggattgtc gcttgctgat cgtgcatgtc tcgcggtcgg
     4981 cctacgtctt gggcttccgg tgctcaccgc tgatcgcaag tgggccgagg tcgacgttgg
     5041 cgtgatggtc aggctcatgc gcacatagag cgccgatacg gcagccgagt gctcgcctcc
     5101 gtatcagcta ggtacgcgtt gcgtcgccca cggtctgccg tccccctacc gtggccgctt
     5161 ggtggcgccc ggcgaggcgc gcccgtccgt ccaggaacgt gctgcccatc agcaggccga
     5221 agccgaggag cccagccgcc gcgagcgcgc tgccggcgac gatcggccag cgcgcggcta
     5281 cgataccttc aacataggcg taccaggcgc tggcgctgag caggacgccc gcgagcaacg
     5341 cgcccagttg tagggcggcg aggcgcgggt gacgcagcgg ccggccggcc aggcgcggga
     5401 acaagtagta accgaagccc gacaccagca gtcccgccca gccgaagagg ttcagctcgg
     5461 cgtgcgtctg gtgcagccgc ggctgccagg cgggcgccac cgccattagc gcgcccaagg
     5521 agacgccgag cgccagcagc aggccggcag ccaccaggcc gaagcgcgtc aggcgtggca
     5581 ggtcaagcgc cagcggcagg atgttccaga cgaaaatcac cagcgcggcg gcctccagcg
     5641 gcccagccac ggcgaagagc ggcgcggcgt cgagcgccaa cgccagcagc atcaccggca
     5701 gcccgaacgc gatcaggaga aaatgcagcc gcagtagcca cggccggcgc cagccgcgcc
     5761 gggaccagcg cggcagcacg tggtagcaga cgcccgagac catgctcagg aaccagccga
     5821 ccagcagcgc gtgcgcccag accagctccc agcgcccgcc gcccggtcgc cagaagcaga
     5881 gcgccaggcc gagtcccagg cctacgagca ggtagactcc ggccagtttg acgaagcggc
     5941 ggccggtagt atcgccatgg acgtgcttct gactcacgcg cgctgccgcc ccgggtgcgg
     6001 gcggagtgcg caccgggccg ccggtggcat aggcgaagac gagcgcggcc gagagacagc
     6061 cggcggcccc gactgcctcc actgtccccc ccaacccggc acggcccaag aagaccaacc
     6121 aggcggactg cgcgaagacg agcgtaagga ggaggaggcg cggcgagcgc cagttgcgcc
     6181 cgctgaaaag cggcagcacg cgcaggttga ccgcatagat caacggcgtg atgccgccga
     6241 gcacgaccag tgcgacaccc gcgggccaca gccgcggttt ccacagcgac acagcgaagc
     6301 tggcctgggc ggttgcccag gccagcagac tgatcatgat cgtgagcggc accaaacggc
     6361 ggccgctggt catgcggcaa gctcgccgaa ggccgcggtc acctcgcgaa cgacctgctc
     6421 gggatccagc ccgtgcagct ccgccgcctc ggccagcgta tgctcgccac cgcagcagag
     6481 atcgaagccg tagctcgcca gtaccggcat caagttcggg tagtcctcca ccagctcgcg
     6541 gatcagtcgc gtctcgatga tctggcgttt gccctcggtg gtcaggccgg tcattgtctt
     6601 cctccaacgc gaggcggcgg gcttgcgccc gccgcccatc ctatcatccg ttcctaatgg
     6661 cccgcgctgc cggcatcgcc cgccttgatg acgttgaaga tgctcgggtc ggcctggcca
     6721 tcgacgtgca gcgtgccgat cgcacccttc atcactcgac ccaggctgtg gtcgaccagc
     6781 gagtagttgc cggggtaatc ggtggtcagc tcgaccatcg tggcgtctcc cggcggcacc
     6841 atggtcgtct gcacgttgtg gttctcgagc gtgccgccct cgacactcac cgtgtcgaag
     6901 ctctcaccga tgatgtggaa gctcgacggc aggttcgggc cgcccactcc gaagaagatg
     6961 cgcaccgtgt cgccgacctt ggactccagc tcgtgctcgc cggtcagcga gccgaccgaa
     7021 ccattgaaga ccacgtactc cggatcctcc gccatcatct tggtcatgtc gaaggtgcgc
     7081 atgcccttgt cgcctacgtt gcccgccgga tagaagtcgc cctgcatcac gtagaactcg
     7141 tgatcgaccg gcggcaggcc ctccggcggc tccaccacga tcaggccgta catgccgttg
     7201 gcgatgtgca tcggcgccgg agccgcggcg cagtggtaga cataggtgcc tgcgtgcagc
     7261 gccttgaaag tgaaggtcgc ctcgctgccc ggcttgaccg aggtcacggc cgcgccgccg
     7321 ccggggccag tcaccgcatg caggtcgata tcgtgcgggc tggtgctcgt cgggtcgttc
     7381 ttgagcgtca gctcgacggt gtcaccctcg cgcacccgga tcatcgggcc ggggacggtg
     7441 ccgttgaagg tgtagaaggt gtagccgacg ccgttgtcga ggagcgcctt gacctccttg
     7501 gccaccaatg tgaccttgac cgtggtcgga tcgcggtgcc cgaccggctg cgccaccacc
     7561 ggctgcggca gctgcgtcag ccccgccacc tcggccgcca gcgtggccgc gtccggcgtg
     7621 gacggcatgt tcatcgccgc ttgcgtgcct cctccgccgg tcgtccaggc gccgaccgag
     7681 gcgacggaga gcgacagaat gaccagcgcg atcagcgcaa gaccacgcat caggccacgt
     7741 cgcatcagat tcatgctcac aacctccttg ggtacacgaa ctcagtcgtc gatcaggttc
     7801 gcgaatcgtc gttcgccggg cgccgaacaa gctgcgccgg caggtgaagc ctgctcgtgc
     7861 tgcgcggcag gcgagtgatc aaggcgcccc cctgccaggg catgccgacc atcggcagca
     7921 gccagcggtc gaagccgagc cagccggcgg tcttccaggc cagcatcagc ccgatcgcca
     7981 ggaccaacag caccggatcg aggctgctgg tgccagccag catgtggctg acgctcagca
     8041 agacgccgag aaaggcggcc agcgcggtga agacgccgag gatcagcgcc agccccacca
     8101 acgtctccac caccgccgcc accggcgcca gccagtggta cgcgccgtgg tcgagcagga
     8161 agtggagcgc tccgcgggac cagccgtcgg ccagcgggtg cgtggcggcg ccctgcgtgt
     8221 gggtcaattg atcccagtag tctcgcagag ccgccccgcc gctcatccag tcg
//
LOCUS       DASUBI010000566         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1081173, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000566 DASUBI010000000
VERSION     DASUBI010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081173"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFU72_10945"
     CDS             complement(<1..1028)
                     /locus_tag="VFU72_10945"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5424049.1"
                     /translation="MLSTAMLTVVSEVAMAAPTLLPACAGVELVELLADEGDITVVVR
                     AYRLSACCPDCGTPATRVHSWYQRRLGDLPWQGLPVAVRLHSRRWFCDAPTCPRRIFT
                     ERFPALVPPHGRRTARLDALVTAVAFALGGRPGARLLAVIGPVVSHDTLINAIRRADL
                     PPAPTPRVLGVDDWSYRRGEVFGTILVDLERHRPADVLPDRTATTFSAWLQAHPGVEV
                     IARDRGGAYADGARQGAPNAVQIADRFHLVKNLGEALERLLDRQHAALRTAARAAAVA
                     EVAAVPAAPSPELAAPASADPTPARPPTRAQRELAQRHARRQARYDEIHRLLAQGMSL
                     WALARHLG"
     gene            complement(1157..1528)
                     /locus_tag="VFU72_10950"
                     /pseudo
     CDS             complement(1157..1528)
                     /locus_tag="VFU72_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          328 a          730 c          667 g          279 t
ORIGIN      
        1 atgcccaggt gccgggcaag cgcccagagg ctcatccctt gcgcgagcag ccggtggatc
       61 tcgtcatacc gcgcctggcg gcgcgcgtgc cgctgggcca gttcgcgctg ggcgcgcgtc
      121 gggggccgtg cgggcgttgg gtccgccgac gcgggcgccg ccagctcggg cgacggtgcg
      181 gcggggacgg ccgccacttc cgccactgct gcggcacggg ccgccgtgcg gagcgcggcg
      241 tgctgccggt ccagcagccg ctccagcgcc tcgccgaggt tcttgacgag gtgaaagcgg
      301 tcggcaatct gcacggcgtt gggtgccccc tggcgcgccc catccgcgta ggccccgccc
      361 cgatcgcgcg cgatgacctc gaccccggga tgcgcctgca accaagcgct gaaggtcgtc
      421 gccgtgcgat cggggaggac atcggcgggg cggtgccgct ccagatcgac gaggatcgtc
      481 ccaaatacct cgcctcgccg gtagctccag tcgtccacgc ccagcacgcg cggcgtcggc
      541 gcgggcggga ggtcggcccg ccggatggca ttgatgaggg tgtcgtggct gacgaccggg
      601 ccgatcaccg cgagcaggcg cgcgccaggt cgccccccga gcgcgaaggc cacggcggtg
      661 accagcgcgt cgagccgcgc cgtgcgccgc ccgtggggcg ggacgagcgc cggaaaccgc
      721 tccgtgaaga tgcgccgcgg gcaggtgggc gcgtcgcaga accagcggcg gctgtgcagg
      781 cgaaccgcga caggtaggcc ctgccagggc aaatcaccga gccgccgctg gtaccagcta
      841 tgcacgcgcg tcgcgggcgt gccgcagtcg gggcaacatg cgctcaagcg gtaggcccgc
      901 accaccacgg tgatgtcgcc ctcgtcggcc agcagttcca ccagttccac gcccgcgcaa
      961 gcgggtagaa gtgtgggagc ggccatggcg acctccgaga cgacggtcag cattgcagtg
     1021 gagagcattc tcccacacgt gaccatgcct agcccggcgc cgccacgggc agcctgggcc
     1081 gactcgccgt caccagatga tcgtcagaac cgcaattagc gcatcgaatg gccgactttc
     1141 cccttgtcaa gcacacctgg accagttcgt ccacccgccg cgtcgagacg ccctggacat
     1201 acgcctcctg caccaccgcc gccagcgcct gctcggcgcg gcggcgcggc tccagcaggc
     1261 tggggaagta gctgccgtcc cgcacgcgcg gcacccggag ctccatcgtg cccacccgcg
     1321 tgtcccactg ccgctcgcga tagccgttgc gctgcccggt ccgctccggc gtgcgctcgt
     1381 gccgctccgc gcccacatgg gcgctcacct ccgcctccag cagtgcctgc gccaacacgc
     1441 gcaccgcctc gcgcaggaaa tccgcgtcgc gctccagccc cgccttgcgc agcagcccca
     1501 gcaggtctat cctctcggcc tcggccatcg tgtaccctcc ttctctcggc gtgtgcaacc
     1561 ggagaggaat cacacggtgg ccgcccgcgt caacggccag ccctcttaca cacttctcgg
     1621 gactctatca tcacgagttg aagccagaca ctacgccgca aacgaagtga tgggttgagc
     1681 gcgacagaat gaccggccac atcatcggca gcgaatccgc tgggcgcccg ttcaaggcga
     1741 tgcaaacgga gtgtcaaccc gcccgagcgg gcgattgtac accggataaa aacatttgaa
     1801 gcagtcactg ttgcgctgcg tccgttgcac gagaagcggc atgcaaaggt gaccatagac
     1861 atgacattcg accgcttagg ggagtggtgc cgccttaccc ttgagcaagc cctcctatcc
     1921 gagaccgagg ccgagacggc aattgggatc actccgtcgg cacccaatca atacgagcgg
     1981 cgcgcagatc gacgaagccg tacc
//
LOCUS       DASUBI010000567         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1347446, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000567 DASUBI010000000
VERSION     DASUBI010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347446"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFU72_10955"
     CDS             <1..584
                     /locus_tag="VFU72_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424050.1"
                     /translation="RETSYLLLANLTRNVAWSAPLVYSVAIWVGVFHLSLRGYSWVFL
                     LAGLTYFAGSNLAPATVRRFTARGVFVVASTLQLAAALAFGLATGNLAVAIFLFGGLV
                     FFAGSFVAVALNILMQDSLPEARGAVLSLSATTQQAGAALGGILGGVMLASLSAGALI
                     PLVSLLIPVAIVALWLSARGFEATPVVESAMGD"
     gene            703..1575
                     /locus_tag="VFU72_10960"
     CDS             703..1575
                     /locus_tag="VFU72_10960"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5424051.1"
                     /translation="MILVVGATGQLGGEIARRLLQRGEAVRMLVRTGSAYQPLAALGA
                     EPVFGDLKDPGSLGPALDDVDVVVTTANSAQRLPPDTIEAVDLRGNASLVDAARAAGV
                     GQFVFISALGAVPNSLSPFLQAKAHTEERLRASGMPYTILEPTIYMEVWAPMIVGLAV
                     AQQRPVILVAGGQRHHSWVSRADVAALTLATIGNPKALNQTILVGGPQPLSWRDIIAT
                     YERVLGRTIPVESVALGEPIPGLPQPIFELLTALETFDASLDMRATQAAYDLPLTPLE
                     TVVHQMTSGAEANG"
     gene            1665..2018
                     /locus_tag="VFU72_10965"
     CDS             1665..2018
                     /locus_tag="VFU72_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424052.1"
                     /translation="MYAGITILLLQPERVDEAIDFLTRLGDEVVIPFARQLPGYRGHL
                     GLADRETARFTTIFLYQTEAQARQMDPTGRLWRHMLEQIAPEQQARLRDLLAAAPERG
                     IAEVNRSALAALGQP"
BASE COUNT          304 a          750 c          722 g          337 t
ORIGIN      
        1 atcgcgagac gagctacctg ctcctcgcca atctgacgcg caatgtcgcc tggagcgcgc
       61 cgctggtcta tagcgtggcg atctgggtcg gtgtcttcca cctctccctg cgcggctaca
      121 gctgggtctt cctgctcgcc ggcctcacct atttcgccgg cagcaatctg gcccccgcga
      181 cggtgcggcg cttcaccgcc cgcggcgtct tcgtcgtcgc ctccacactg cagctcgccg
      241 ccgcgctcgc cttcgggctg gctaccggca acctggccgt ggccatcttc ctcttcggcg
      301 gcctcgtctt cttcgccggc tccttcgtcg cggtggcgtt gaacatcctc atgcaggaca
      361 gcctgccgga ggcacgcggc gcggtgctca gtttgtcggc cacgacccag caggccggcg
      421 ccgcgctcgg cggcatcctc ggcggcgtca tgctggccag cctctcggcc ggggcgctga
      481 tcccgctcgt cagcctgctg attcccgtgg cgatcgtcgc tctctggctg agcgcacgcg
      541 gcttcgaggc cactccagtg gtggaaagcg cgatgggaga ctagacaatc gtgacgaatt
      601 gcgtgacaat cacgctagcg attctcaacc gatgaattcg atccgctgtc ggcaggcgag
      661 cctagaggaa cgcccgccgg gggcccccag aggaggcacg ccatgatcct ggtcgtcggc
      721 gccaccggac aactgggcgg cgagatcgcc cgccgcctgc tgcagcgcgg tgaggcggtg
      781 cgtatgctgg tgcgcacagg ctccgcctat cagccgctgg ccgcgctcgg cgccgagccg
      841 gtcttcggcg acctgaaaga cccaggctcg ctcggcccgg cgctggacga cgtggacgtg
      901 gtggtcacga ccgccaactc ggcgcagcgc ctgccgcccg acacgatcga ggcggtcgac
      961 ctgcgcggca acgccagcct ggtagacgcg gcgcgggcgg ccggcgtggg ccagttcgtc
     1021 ttcatctccg cgctgggcgc cgtccccaac agcctgtcac cgttcctgca ggccaaggcg
     1081 cacaccgagg agcgcctgcg cgccagcggc atgccctaca ctatcctgga gccaaccatc
     1141 tacatggagg tctgggcgcc gatgatcgtt gggctggccg tggcgcagca gcggccggtg
     1201 attctggtcg cgggcgggca gcgacaccac tcctgggtct cgcgcgccga cgtggccgcg
     1261 cttacgctgg ccaccatcgg caaccccaag gcgctcaacc agacgatcct ggtcggcgga
     1321 ccccagccgc tctcctggcg cgacatcatc gccacctatg agcgggtgct cgggcggacg
     1381 atcccggtgg agagcgtggc gctgggcgag ccgattccgg ggctgccgca gccgatcttc
     1441 gagctgctca ccgcgctgga gacgttcgac gcgtcgctcg acatgcgggc gacgcaggcc
     1501 gcctacgacc tgccgctgac cccgctggag acggtcgtcc accaaatgac gagcggggcg
     1561 gaggcgaacg ggtaagcgtt cgggacgtgc ggcagcggga acgattggtg cgagaatcgg
     1621 cagtgtgagc cgcgcatggc ggctgcttgg accagggagc cgacatgtac gccgggatca
     1681 ccatcctgct gctgcagccc gagcgcgtgg acgaggcgat cgactttctg acgcgcctgg
     1741 gcgacgaggt cgtcatcccc ttcgcgcggc agttgccggg ctatcgcggc cacctggggc
     1801 tggccgaccg cgagaccgcg cgcttcacga ccatcttcct ctaccagacc gaggcccagg
     1861 cgcgccagat ggaccccacg ggccggctct ggcgccacat gctggagcag atcgcccccg
     1921 agcagcaggc gcgactgcgc gacctcctgg ccgccgcgcc cgaacgcggc atcgccgagg
     1981 tcaaccgctc tgccctggcc gcactcggcc aaccgtaggg tggctcgtga agacgtggcc
     2041 tggtgccgcg tggggcacgg atccttcgac aagctcaggt tgacatgttt gggggattgg
     2101 ggcggacgaa tac
//
LOCUS       DASUBI010000568         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_314743, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000568 DASUBI010000000
VERSION     DASUBI010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314743"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFU72_10970"
     CDS             complement(<1..1028)
                     /locus_tag="VFU72_10970"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5424053.1"
                     /translation="MPKYIFITGGVVSSVGKGITTASIGRLLKSRGLKVAIMKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTDENLSRASNVTTGQIYSAVIANE
                     RRGDYLGGTVQVIPHITNEIKERIRLVAEQHQPDVVIVEVGGTVGDIEGQPFLEAIRQ
                     MRKDAGRHNVLYIHVTLLPKVGSTGELKTKPTQHSVKELREIGIQPDVIVVRSDYPVP
                     DEVRAKISLFGDVDEEAVIPLPTAETIYEVPLELEEAGLGRYLADHFGWQDRSPDLAR
                     WQELVCQIKQPKRGIRIALVGKYVELHDAYMSVAESLMHAGLRHGLDVEIVWVNSEQE
                     TPEEIERKL"
     gene            complement(1074..1709)
                     /locus_tag="VFU72_10975"
     CDS             complement(1074..1709)
                     /locus_tag="VFU72_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424054.1"
                     /translation="MAEITWLGHACFRIRGREAVVLTDPIEPEAGLVAGEQTADIVTV
                     SRVLDEGESLAGVGPKTRIVAGPGEYEIADVFITGIRSHRDAEQGAQRRANTIYLIEI
                     EDLVICHLGNLGQALSEEQAGGMSAVDVLIVPVGGEQTIDAAAAVEVIGQIEPSIVIP
                     MPDSSERGNGDREPLDRFLKQVGSADVVPVPRLSVKKSDLGETMQVVVLQR"
     gene            complement(1829..2257)
                     /locus_tag="VFU72_10980"
     CDS             complement(1829..2257)
                     /locus_tag="VFU72_10980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5424055.1"
                     /translation="MSEPSAPIRYWNRQQVRYRDVDMQQIVYFAKYLEYVDIALDDYL
                     RSLGVVVSETAPSGEYDTAMVHVELDYLAPARYDDVIDVGIRIERLGRSSMDARFEIQ
                     RAGLPLVRGAMVLVNFDRDSGRARDLPERLRALIREREDF"
     gene            complement(2254..2916)
                     /locus_tag="VFU72_10985"
     CDS             complement(2254..2916)
                     /locus_tag="VFU72_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEU5424056.1"
                     /translation="MANIRSGTITAIEVQARDPERVNVFIDGSFAFGVDQLVAAEHDL
                     QAGKTLAPADVAKLLAADESSRATAAALQFLGYRPRSRAEVERRLARRGFAPEAIVAA
                     VERLERWRYLDDDAFARAWIENRQEHQPRGRRRLKDELRQKGVDREVVEQALEEAELD
                     EYPAALELARKRLPSLRGDEPLAQRRRLAGFLQRRGYGWDVVGRVLDAALGEPDDNEE
                     LA"
     gene            2986..>3171
                     /locus_tag="VFU72_10990"
     CDS             2986..>3171
                     /locus_tag="VFU72_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424057.1"
                     /translation="MARSTPRSLTGSTKPRAYERKRSRSMRRWVLVIGATMLLLVLAA
                     VVLTPVFLYAAVMHAARQ"
BASE COUNT          505 a         1117 c          988 g          561 t
ORIGIN      
        1 agcttgcgct cgatctcctc cggcgtctcc tgctccgaat tgacccagac gatctcgaca
       61 tcgagcccgt ggcgcagtcc cgcgtgcatc agcgactcgg ccacgctcat gtaggcgtca
      121 tgcagctcga cgtacttgcc caccagcgcg atccgaatgc cacgcttcgg ctgcttgatc
      181 tggcagacca gctcctgcca gcgcgccaga tcggggctgc gatcctgcca gccgaaatgg
      241 tcggccaggt agcggcccag tcctgcctcc tccagctcca gcggaacctc gtagatcgtc
      301 tccgcggtcg gcagcgggat cacggcctcc tcatcgacat cgccgaagag cgagatcttg
      361 gcgcgcacct cgtcgggtac cgggtagtcc gagcgcacca cgatcacatc tggctgaatg
      421 ccgatctcgc gcagctcctt gacgctgtgc tgggtcggct tagtcttcag ctcgccggtg
      481 ctgcccacct tcggcagcaa ggtgacgtgg atgtagagca cgttgtgccg cccggcgtcc
      541 ttgcgcatct gccggatggc ctccaggaac ggctggccct cgatgtcgcc caccgtgccg
      601 ccgacctcga cgatcacgac atccggctgg tgctgctccg cgaccaggcg gatgcgctcc
      661 ttgatctcgt tggtgatgtg cgggatcacc tgcaccgtgc caccgaggta gtcgccgcgg
      721 cgctcgttgg cgatcaccgc tgaatagatt tgcccggtgg tgacattgga ggcacggctc
      781 aggttttcat cggtgaagcg ctcgtagtga ccgagatcga ggtcggtctc cgcgccgtcg
      841 tcggtgacga agacctcgcc gtgttgatag ggcgacatgg tgcccggatc gacgttgaga
      901 tagggatcga gcttcatgat cgcgaccttc aggccgcggc tcttgagcag gcgcccgatc
      961 gatgcggtgg tgatcccctt gcccaccgat gaaacgacgc cgcccgtgat aaagatgtac
     1021 ttcggcatgc tcccccagcc ttcccggctc ctacccggcg cgactcctct cggctagcgt
     1081 tgcaggacga ccacctgcat cgtttcgccg agatccgact tcttcacgct caagcgcggc
     1141 accggaacca cgtcggcgct gccgacctgc ttgaggaagc ggtcgagcgg ctcgcgatct
     1201 ccattgccgc gctcgctgct gtcgggcatc ggaatgacta tggatggctc gatctggccg
     1261 atcacctcca ccgccgccgc ggcgtcgatc gtctgctcgc cgccgaccgg cacgatcagc
     1321 acgtcgaccg cgctcatgcc gccggcctgc tcttcggaca gcgcctgccc caggttgccc
     1381 aggtggcaga tgaccagatc ttcgatttcg atgaggtaga tggtgttggc gcgacgctgc
     1441 gcgccctgct cggcgtcgcg gtgcgagcgg atgccggtga tgaagacatc ggcgatctcg
     1501 tactcgcccg gcccggccac gatccgggtc ttcggcccca cgccggccaa cgactcacct
     1561 tcatcgagga cgcggctgac ggtgacgatg tcggcggtct gctcccccgc tacgagcccg
     1621 gcttccggct cgatcgggtc ggtcagcacg accgcttctc ggccacgaat gcggaaacac
     1681 gcatggccca accaggtgat ctcagccatc tcctgacatt ccgatgcaac gacctattcg
     1741 gctgtccaat gggcataact gggaggttgt cccgcggcct atacaagtat acccgacagc
     1801 gtccggacat accagggcaa ccggggatct agaaatcctc acgctcccga atcagcgcac
     1861 gcagccgctc aggcaagtcg cgggcccgcc cggagtcgcg gtcgaagttc accaacacca
     1921 ttgcaccgcg caccagcggc aagccggcgc gctggatctc gaaacgcgcg tccatgctgg
     1981 agcggcccag gcgctcgatc cggatgccga catcgataac gtcgtcgtag cgcgcgggag
     2041 ccagatagtc gagctcgacg tggaccatcg ccgtgtcgta ttcgccgctc ggggcggtct
     2101 cgctgacgac cacgcccagc gagcgcaggt agtcgtccag cgcgatgtcg acgtattcca
     2161 gatacttggc gaagtagacg atctgctgca tgtcgacgtc gcgatagcgg acctgctgcc
     2221 ggttccagta gcggatcggc gcgcttggct cgctcatgcc aactcctcgt tgtcgtccgg
     2281 ttcgccgagc gccgcgtcca gcacgcgacc gaccacgtcc cagccatacc cgcgccgctg
     2341 gagaaagccg gccaggcgcc ggcgctgggc cagcggttcg tcgccgcgca ggctcggcag
     2401 ccgcttgcgc gccagctcca gcgcagcggg atactcgtcc agctcggcct cctccagcgc
     2461 ctgctcgacg acctcccgat ccacgccttt ttggcgcagc tcatccttca gccgccgccg
     2521 gccgcgcggc tggtgctcct gccggttctc gatccaggca cgcgcgaagg cgtcgtcgtc
     2581 gagatagcgc cagcgctcca ggcgctcgac tgccgccaca atcgcctcgg gcgcgaaccc
     2641 gcgccgagcc aaccgccgct cgacctcggc ccgcgagcgc ggacggtagc ccagaaactg
     2701 caaggcggcc gccgtggcgc ggctcgactc gtcagcagcc agcagtttgg cgacgtcggc
     2761 cggcgccagc gtcttgccgg cctgaagatc gtgctcggcc gccaccaact ggtcgacacc
     2821 gaaggcaaac gagccgtcga tgaaaacgtt cacgcgctcg ggatcgcgcg cctgtacctc
     2881 gatcgcggtg atggtgccag aacggatgtt cgccatctcc atgcccctag tatgccagaa
     2941 gtgcggacgt gggtttccgg gcaggcgtgg tgtaccatct gacgcatggc tcgaagtacg
     3001 ccgcgatcgc tgaccggttc caccaagccg cgcgcgtacg agcgcaagcg ctctcgctcg
     3061 atgcgccgct gggtgctggt catcggcgcc accatgctcc tgctggtgct ggcggccgtg
     3121 gtgctgacac cggtcttcct ctacgcagcg gtcatgcacg ctgcccgcca g
//
LOCUS       DASUBI010000569         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_431552, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000569 DASUBI010000000
VERSION     DASUBI010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431552"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFU72_10995"
     CDS             complement(<1..504)
                     /locus_tag="VFU72_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424058.1"
                     /translation="MTRYIVRRVLTAIPTLIAISMVIFTILALAPGDPLSEFALNPAI
                     PPSVRQNIRKSMGLDDPIPVRYAKWFKSVMTGDFGYSFTSKSPVMGLIKQRLPTTLWV
                     IGSAYLLSVLIAIPIGVLSAIKQYSIMDNVATTIAFMGFSLPTFFTGLLAIMLFTIWL
                     GWLPMIYS"
     gene            complement(727..2691)
                     /locus_tag="VFU72_11000"
     CDS             complement(727..2691)
                     /locus_tag="VFU72_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5424059.1"
                     /translation="METPELKRLYAQAASGKLTRRQVLRRAAALGLSAPAISLLLAAC
                     GGSSSKTTATTSSAATTAPTSAATTAAGGTTPAATKAATSAAGTPSAAGSPTGGATAA
                     NVKVLTSTGSRGDQGELKLLWWQAPTILNSHLAQGTKDYDASRPVLEGLADFDSDGNF
                     IPLLAAEIPSLDNGGVSKDGKTVTWKLKQGVKWSDGQPFSSADVKFTYDYVIDKTTTS
                     VSYAYYTAIDSIDTPDDHTVVIHFKDPTPAWYGGIFCGDYGRILPQHVLKDFTGAKAR
                     SAPFNLQPIGTGPYKVDSFKPGDVVQYSMNENFREANQPHFKTVSLKGGGDATSAARA
                     AIQTGEVDFAWNLQVDWSVLSTLESNSSPGKLLVNPGNGVERILINLTDPNKEVNGER
                     SHLGTPHPFQSDLNVRKAYALLVDRDTMAKQLYGQTGNATANLLTAPQKFVSKNTSFK
                     FDPATAQTMLDTAGWKKGGSGVREKDGVQMKIVYQTSVNTLRQKEQEIVKQAFDAAGI
                     QVQLKSIDASVYFSSDAGNNDTASHFYADLEMFTNSGSSPYPIDYMSSWWGDPSNIAQ
                     KSNEWAGSNTERWQNADYDAAYEQAKVELDPAKQADLFIKMNDLIVNNVVDIAEIQRN
                     GASGVNKKLQNVVLSIWCSNLMNIANWTMG"
     gene            complement(2844..3956)
                     /locus_tag="VFU72_11005"
     CDS             complement(2844..3956)
                     /locus_tag="VFU72_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424060.1"
                     /translation="MVDVGETAQALARRNILDLHEIAGGLEPAERERFNRLFAVESTA
                     GRLVPPEHMHRWVERYFGSVAAVASQKVVKVTNRVTLEETLFNELRARRPLEARIPAE
                     LAEEIARTAPDPFCEPELNTPADTFGRVRGSHAITASNIAKYDGAHGVIIFGDHDPLA
                     FTEDSVVDVVRVAQEWFVQAHAADPTAVYPLFMWNCLWKSGASIPHGHAQVSLTRGMH
                     YGKVERQRRVAQDYQQAHGENYFDDLFRTHQALGLGARLGQVRVMASLTPLKEKEVVL
                     LAPRVDDQLARVVHDTLQCMVDQLNVVSFNLVIWQPPLAETPEDWSGMPVIVHAVDRG
                     DPLNRTSDIGAMELYAASVVSSDPFRVAEAVLPRLG"
BASE COUNT          697 a         1312 c         1430 g          800 t
ORIGIN      
        1 actgtagatc atcggcagcc agcccagcca gatggtgaag agcatgatgg ccagcaggcc
       61 ggtgaagaag gtgggcagcg agaagcccat gaaggcgatg gtggtggcga cgttatccat
      121 gatcgagtat tgcttgatcg ccgagaggac gccgatcggg atggcgatca agaccgagag
      181 gagataggcg ctgccgatca cccacaaggt ggtaggcagg cgctgcttga tcagccccat
      241 caccggcgac ttgctggtga aggagtagcc gaagtcgccg gtcatgaccg acttgaacca
      301 cttggcgtag cgcaccggga tgggatcgtc gagccccatc gacttacgga tgttctggcg
      361 caccgagggc gggatggccg gattgagcgc gaactcggac agcggatcgc ccggcgccag
      421 cgccaggatg gtgaagatca ccatgctgat cgcgatcagc gtggggatgg ccgtcagcac
      481 gcgacgaacg atgtatcgcg tcattcagtt ctctcctgcc tgggctcgaa agctcgtgtc
      541 ccccgcgatc gcgccagagt gggcgtggat ggctacgagt atgcgaactc ccgtggcgct
      601 gcacgggatg atacggatcg gcggacgctc gctgcaacta agaatgcccc gaaagtcctg
      661 atcaagcaag accctgggcg aaaatggccg gagcgaggac gctcctcgct ccggcctgat
      721 ccgctgctag cccatcgtcc agttggcgat attcatgagg ttcgagcacc agatcgagag
      781 gaccacgttc tggagcttct tgttcacccc ggaggcgccg ttgcgctgga tctccgcgat
      841 gtcgaccaca ttgttgacaa tgaggtcgtt catcttgatg aacaggtccg cctgcttggc
      901 cgggtccaac tcgaccttgg cctgctcgta ggcggcatcg tagtcggcgt tctgccagcg
      961 ctcggtgttg ctgcccgccc actcgttcga cttctgggcg atgttcgagg ggtcgcccca
     1021 ccacgacgac atatagtcga tcgggtacgg ggaggagccg ctgttggtga acatctccag
     1081 gtcggcgtag aagtggctgg cggtgtcgtt gttgccggcg tcggacgaga agtagaccga
     1141 agcatcgatc gacttgagct gaacctggat gccggccgca tcgaacgcct gcttgacgat
     1201 ctcctgctcc ttctggcgca aggtgttcac cgacgtctgg tagacaatct tcatctggac
     1261 gccgtctttt tcgcggacgc cggagccgcc cttcttccaa ccggcggtgt cgagcatcgt
     1321 ctgcgcggtg gcggggtcga acttgaatga ggtgttcttg gagacgaact tctgcggcgc
     1381 ggtcagcagg ttagccgtgg cattgcccgt ctgtccgtag agctgtttgg ccatcgtgtc
     1441 gcggtcaacc aacaaggcgt aggccttgcg cacgttgaga tcggactgga aggggtgcgg
     1501 cgtgcccagg tgcgagcgct cgccgttcac ctctttgttc gggtcggtca ggttgatcag
     1561 gatccgctcg acgccattgc ccggattgac cagcagcttg ccggggctgc tgttggattc
     1621 gagggtggag agcaccgacc agtcgacctg caggttccag gcgaaatcga cctcgccggt
     1681 ttggatcgcg gcgcgggccg cgctggtggc gtcgccgccg cccttgagcg agacggtctt
     1741 gaagtgcggc tggttagcct cgcggaagtt ctcgttcatc gagtactgga ccacgtcacc
     1801 gggcttgaag ctgtcgacct tgtaggggcc ggtgccgatc ggctggagat tgaagggcgc
     1861 gctgcgcgcc ttggcgccgg tgaagtcttt gagcacatgc tggggcagga tgcggccgta
     1921 gtcaccgcag aagatgccgc cgtaccaggc cggggtcgga tccttgaaat ggatcacgac
     1981 ggtgtggtcg tcgggggtgt cgatcgagtc gatcgcggtg tagtacgcat agctcacgga
     2041 ggtcgtggtt ttgtcgatga cgtagtcgta ggtgaacttc acatccgcgg aactaaaggg
     2101 ctgcccgtcg gaccacttga cgccctgctt gagcttccag gtgaccgtct tgccgtcttt
     2161 ggagacgccg ccgttgtcca gcgaggggat ctcggccgcg agcagcggaa tgaagttgcc
     2221 gtcggagtcg aagtcggcca ggccctctag tactgggcgg gaggcgtcgt agtccttggt
     2281 gccctgcgcc aggtgcgagt tgaggatcgt gggcgcctgc caccacagga gcttgagctc
     2341 gccctggtcg ccccggctgc cggtgctggt cagcaccttg acgttggcgg ccgtggcacc
     2401 accggtggga ctgcccgccg cggacggtgt gccggcggcg ctggtcgcgg ccttggtcgc
     2461 cgcgggggtc gtaccgccgg cggcggtggt cgccgcgctc gtcggggccg tggtcgccgc
     2521 cgacgaggtg gtcgccgtgg tcttgctcga gctgccgccg caggcggcca gcagcagtga
     2581 gatagcgggc gcgctcaggc cgagcgcggc ggcacgccgg agcacctggc gccgggtcag
     2641 tttgccgctg gccgcctgag cgtagagccg tttaagctct ggtgtctcca tgacgatctc
     2701 ctcctgtgtt gcgcgagcaa cattgctaag tacccactcg cgccgaagcg gctactccac
     2761 gcacgccgct acgccgcccg aactgccaca tttttcgggc aatttacgcg attatgagcc
     2821 tggagaaggc gttcgtcaag atgtcagccc agccgcggca gcaccgcctc ggctacccgg
     2881 aacggatcgc tggagacgac gctggcagcg tagagctcca tcgctccgat atccgaggtc
     2941 cggttgagcg ggtcgccacg gtccaccgcg tggacgatca ccggcatgcc gctccaatcc
     3001 tcgggtgtct ccgccagggg cggctgccag atcaccaggt tgaaggagac gacgttgagc
     3061 tgatcgacca tgcactgcag cgtgtcgtgg acgacgcgcg ccagttggtc gtcgacgcgc
     3121 ggcgccagca gcacgacttc cttctccttg agcggtgtca ggctcgccat gacgcgcacc
     3181 tggccgagcc gggcacccag ccccaacgcc tgatgggtac gaaagaggtc gtcaaagtag
     3241 ttctcgccgt gcgcctgttg gtaatcctgc gccacccgcc gctggcgttc gaccttgccg
     3301 taatgcatgc cacgggtcag gctgacctgg gcgtgaccat gcggaatcga ggcgccgctc
     3361 ttccagaggc agttccacat gaacagcggg tagactgccg tgggatcggc cgcgtgggcc
     3421 tggacgaacc actcctgggc cacacgcacc acgtcgacca ccgagtcctc cgtaaatgcc
     3481 agcgggtcgt gatcgccgaa gatgatcacg ccgtgcgcgc cgtcgtattt ggcgatgttg
     3541 ctggcggtga tggcgtggct gccacggacg cgcccgaagg tatcggccgg cgtattcagc
     3601 tcgggctcgc agaagggatc gggcgcggta cgcgcgatct cttcggccag ctccgcgggg
     3661 atgcgcgcct ccaacgggcg cctagcgcgc agttcgttga agagggtctc ctcaagcgtc
     3721 acccgattcg tgaccttgac gaccttctgc gacgcgaccg ccgccacgga gccgaagtag
     3781 cgctccaccc agcggtgcat atgctccggc ggcaccaggc gtccggcggt cgattcaacc
     3841 gcgaacagac ggttgaagcg ctcccgctcg gctggctcca gcccgccggc gatctcatgt
     3901 aggtccagaa tattgcggcg cgccagggcc tgcgccgtct ctcctacgtc caccacgaat
     3961 gatgctctct ctctgattca ggttgttgtt tgggtcgaac gtcgcgcgtg ctcgcccctc
     4021 aatccgcgaa gtatacaccg cgccggtgta cggacactgc aggtcgcggc gccaccggac
     4081 gtattcgtca ggccgcgcct cgacagccac cgtctctcac ccgagccgcc gaggcggttg
     4141 ccccagcgcc ggccgctacg cccacagcgc gatcgtgcgc gcggagtcga tcgtctcgcc
     4201 gctgacaact tcgccctcgg ggaagtccga ctctttcac
//
LOCUS       DASUBI010000570         5996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_607525, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000570 DASUBI010000000
VERSION     DASUBI010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607525"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1296)
                     /locus_tag="VFU72_11010"
     CDS             complement(100..1296)
                     /locus_tag="VFU72_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5424061.1"
                     /translation="MATTLTLRDEIDEWLPELIATRRDLHRHPELGLEEMRTSAIVAE
                     RLRAIGFDDVRTGIAVTGVKAVLRGGRPGKTLLLRADMDALPIEEENDVDYRSTTAGM
                     MHACGHDGHTSILLSAARMLYVRRAEIPGTIVFCFQPAEEGRGGARRMIEDGVLEDPH
                     VDAAIGLHLISNIPVGTAVTVDGPALSGANRFSATIQGSGGHGAMPHRTIDALLIACQ
                     VVSTLQILVAREVDPQQPAVISVGTLHAGTTAANIIADTATLSGTVRWFDAEVGDQLA
                     RRVPEVIQGIAASLGGRAEVEYRRGGRPTVNDPAVAALVREAVAEVVGAEHSLSGPPI
                     MGSEDFSEFTHRVPSAFFFVGCRDEASGKAFEHHHPRFDIDERSLPLGVEMLTRAALA
                     RRQRRD"
     gene            1574..2395
                     /locus_tag="VFU72_11015"
     CDS             1574..2395
                     /locus_tag="VFU72_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424062.1"
                     /translation="MSSTPLQIEINGIPMAVDVSGSGPAIVFLHAGIADRRMWEPQQR
                     FFAGDYRVISYDLRGFGETPLEPGPFSPRADLRDLLVRLGAAPAVIVGSSFGGKIALE
                     AVVQYPEIARALVLVGAPLGGYDWGAAFEADEAEIEAACTAGEFERAAAVDLRLWVDG
                     PYRTAADLEPAFREQARRLARHVYEVAVDVDARPEPLEPPAVERLGAVRVPTLVVVGE
                     LDQPAMREMAEVMANGIPGARLIAIPNAAHLPSLERPEVFNHILGQFLAGMVPTA"
     gene            complement(2502..3101)
                     /locus_tag="VFU72_11020"
     CDS             complement(2502..3101)
                     /locus_tag="VFU72_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424063.1"
                     /translation="MAGVSRRPSRWLYLLAVLIIALGGTIFALLMVAPFRGMQRVMMP
                     GTQTVQLTRSGTHMLFYEYEGSLHGVSYSAAHDPVGLQVSVVDTASGQAVPTIAVPHS
                     RSQYNLQSRSGYSLARFEVDQPGAYAVTATYPNGQAGPAFALAIARGSFGGVLSGIAA
                     GVVVLVLSLLAGIGLAGWTFSERHQGWLGPGGHEPAAPA"
     gene            3424..4245
                     /locus_tag="VFU72_11025"
     CDS             3424..4245
                     /locus_tag="VFU72_11025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5424064.1"
                     /translation="MQRISSRQPLLLGIGVTLTFMFGLLFAVLVLPDSVVPAGKVAIR
                     MLVPVALLTILGLWRTAGFTRRPTWSNLWPFLPLLLIPLVPAIFGTGVTITDPAEIAL
                     LVLMNFTVGFGEEATFRGVVLRVLAPRGLMRAAVLSSVLFGAMHLVNLAVGEHPVNVG
                     FQVIYTVELGFAYASAALVTGAIWPLVLIHFAQDFANAIQTSNTGSGSPGGVDLASGL
                     INVALWLPFVVYGYWLLRHHLRQSGSEDERPGPRVPAMSAEDVTLGRGRAWRSAG"
     gene            4265..5188
                     /locus_tag="VFU72_11030"
     CDS             4265..5188
                     /locus_tag="VFU72_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU5424065.1"
                     /translation="MHAQRMRSTLTKDSGSRGDTGRPGSPDQRISLKVFFALAFGLGW
                     GIAILMLLFQTQIEAVFGEISGTNPVFILVVYSPAIAGIYLVWRHYGVKALGSFFRRL
                     TMWRLPMAWWLFVLLGIPAVKYLGAAFNGTATEFPFSPWYDVLPALAAALLIGPVEEF
                     GWRGVALPLLQRRFVPLAASLILGAFWGLWHLPAFMFSGTPQSAWSFGPFLIGVMALA
                     VILTPIFNAARGSMLFPILFHFLINGPMWPEAQPWENFIFAALAVAIVALNWQTMTTR
                     GGAATEILTPGAEDVGAGAVETRTAMTRAGA"
     gene            complement(5325..>5996)
                     /locus_tag="VFU72_11035"
     CDS             complement(5325..>5996)
                     /locus_tag="VFU72_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU5424066.1"
                     /translation="AAHLLDALGGELPANLTLSRIDPAVESARYRQEVLRRAAGQEPD
                     ALALLEEDLRSPCTEEIAVFQAFSRTIAQARNRFVVLDTAPTGHTLLLLDATGAYHRE
                     IVRKAGQLPGRLTTPLMRLQDPSFAHVLIVTLPEGTPVLEAARLQEDLRRAGIEPYGW
                     IVNQLLATSGTAHPLLAERARQELRQVHEVAATLASRLWTVPWQAEPLVGAARLRTLT
                     TASTN"
BASE COUNT          881 a         2068 c         2027 g         1020 t
ORIGIN      
        1 tcgccgcgtg attcgacgat cacgcggcga ggtcggtctc cagcttgccc tcaacgcggc
       61 gacaggtatg cacgtggtgg cctgggccag tcggttcaat caatcgcggc gttgacgtcg
      121 agccagcgca gcgcgggtca gcatctcgac gccgaggggc agcgagcgct catcgatatc
      181 gaagcgcggg tgatgatgct cgaaggcctt gccggatgcc tcgtctcgac agccgacaaa
      241 aaagaaggca gacggcacgc ggtgggtgaa ctcggagaaa tcttccgagc ccatgatggg
      301 cggaccggac aaggagtgct ccgcgccgac cacctcggcc accgcctcgc gaacgagcgc
      361 cgcgacggcc ggatcgttga cggtgggacg cccgccccgc cgatactcga cctcggctct
      421 gccgcccagc gaggccgcga tcccctgaat cacctccggg acgcgccgcg ccaactgatc
      481 gccaacctcg gcatcgaacc agcgcaccgt gccgctgagg gtggcggtgt cggcgatgat
      541 gttggcggcg gtcgtgccgg cgtgcagcgt gccgaccgag atgacggcgg gctgctgcgg
      601 gtcgacctcc cgcgcgacga gtatctgcag ggtggagaca acctggcagg cgatcagcag
      661 ggcatcgatc gtccgatgcg gcatcgcgcc gtggccgcca ctgccctgga tggtggcgct
      721 gaatcggttc gcgccggaga gggccggacc atcgacggtc accgccgtgc cgaccgggat
      781 attgctgatg aggtgcagcc cgatcgccgc atcgacgtgc gggtcttcga gaacgccgtc
      841 ctcgatcatc cggcgcgcgc cgccacgccc ctcctcggcc ggctggaagc agaagacgat
      901 cgtgccgggg atctcagcac gccgcacgta gagcatccgt gccgcgctga gcaggatgga
      961 cgtgtggcca tcgtgcccgc aggcgtgcat catccccgcg gtcgttgagc ggtagtcgac
     1021 gtcgttctct tcctcgatcg gcaacgcgtc catgtcggcg cgcagcagaa gcgtcttgcc
     1081 cggcctgccg ccacgcagga ctgccttgac gccggtcacg gcgatgccgg tacgcacgtc
     1141 gtcgaagcca atcgcccgca ggcgctcggc aactatggcg ctcgtgcgca tctcttccag
     1201 ccccagctcc gggtgtcggt ggagatcacg ccgcgtcgcg atcagctcgg gcagccactc
     1261 gtcgatctcg tcgcgcaacg tcagtgtcgt agccatgctg ggtaatccct tccctcccga
     1321 tccgtggcgc agtatagcag cgccgctcca atcgtcgcgg cgcgctggcg cgatccggca
     1381 tagcatcccc gagcgcttcc gcagccactc cgtcgcttcc tgggaacgcc tgcaggacgt
     1441 aagatccagt gcgcacgccg gttcctggag tccctgaacc gtgactgcgt ccctcatccg
     1501 ctggagcagg tactgttgtc cgcgagcggc tgaatccctc acttggccgc gactcagcag
     1561 ggcgaatcat cagatgagca gcacgccgct ccagatcgag atcaacggca tccccatggc
     1621 ggtcgatgtg tcggggagcg gcccggcgat cgtcttcctg catgccggca tcgccgaccg
     1681 gcgcatgtgg gagccgcagc agcggttctt cgccggcgac taccgggtca tcagctacga
     1741 cctgcggggc ttcggtgaga ccccgctgga gccgggtccg ttctcgccgc gcgccgatct
     1801 ccgtgacctc ctcgtgcggc ttggggcggc gccggccgtc atcgtcggca gctccttcgg
     1861 cggcaagatc gcacttgagg cagtcgtgca gtatcccgag atcgcccgcg cgctcgtgct
     1921 ggtcggcgca ccgctcggtg gttacgactg gggcgccgcg ttcgaggcgg acgaggcgga
     1981 gatcgaggcg gcgtgcacgg cgggcgaatt cgagcgcgcg gccgcggtcg atctgcggct
     2041 ctgggttgac gggccctatc gcacggcagc cgaccttgag cccgccttcc gtgagcaggc
     2101 gcgacggctg gcgcgacacg tctacgaggt cgcggtcgac gtcgacgcgc ggccggagcc
     2161 gctggagccg ccggctgtcg agcgtctggg agcggtgcgc gtgccaaccc tggtcgtagt
     2221 cggtgagttg gaccaaccgg cgatgcggga gatggcggag gtgatggcga acgggattcc
     2281 cggcgctcgc ctcatcgcca tcccgaacgc cgcgcacctg ccgagcctgg agcggccgga
     2341 agtgttcaat cacatcctgg gccagtttct cgccggtatg gttcccactg cgtgactctc
     2401 caaagaacgt ccgctggagc acccgagttg ggggtagggg cgccacgagg gaaccacctc
     2461 ccctatgctg agcctctacc gcgtcgtgcc tcccacgtgc tctaggccgg agcggccggt
     2521 tcgtgcccac cggggcccag ccacccctgg tggcgctcgc taaaggtcca tcctgccagg
     2581 ccgatgcccg ccagcagcga caggacgagg accaccaccc cggccgcgat gccgctcaag
     2641 acgccgccga agctcccccg cgcgatcgcc agcgcgaacg ccggcccggc ctgaccgttc
     2701 ggataggtcg cggtaaccgc gtaggcgccg ggctggtcga cctcgaagcg cgcgagcgag
     2761 tagccgctgc gcgactgcag gttgtactgg ctacgactgt gtggcaccgc gatggtcggg
     2821 accgcttgcc cgctggcggt gtcgaccacc gagacctgca acccgaccgg gtcgtgggcc
     2881 gcgctgtagc tgacgccgtg cagggatcct tcgtactcgt agaagagcat atgcgtccca
     2941 ctccgcgtga gctgcaccgt ctgcgtcccc ggcatcatca cgcgctgcat gcctcggaac
     3001 ggcgccacca tcagcagcgc gaagattgtc ccgccaagcg cgatgatcag cacggccagc
     3061 agatacaacc accggctcgg ccgtcgcgat acccctgcca tgccatccat ccttcctcgt
     3121 ggctcccgtg tcagggaacc ggccgcaagg gtagcccctg gtcagtcaca gcgcggtgac
     3181 atcgaccgta gccgtgcaac aggttcgttc caagatcgga gccggcgcgc gcctgtcgtc
     3241 gggcgggcca gccacgcgat gcggtgggcc agcagggcat gcgattgaca cgacggacct
     3301 cggttgtgtt acccgcctgt cgcgctggag cgagtacggt ggtaccgggt gaagcctcct
     3361 gctcggcgcg gtctgggtga cttccccggc attcctcaga ccgtgcgaac ggaaaggtcc
     3421 atcatgcagc gcatcagcag ccgtcagccg ctgttgctcg gcattggcgt gacactcacc
     3481 ttcatgttcg ggctcctctt cgcggtgctc gtgctcccgg acagcgtcgt tccagccggt
     3541 aaagtggcga ttcggatgct cgtcccagtc gccctcctga ccatcctcgg cttgtggcgc
     3601 acggccggct tcacccggcg gcccacctgg tcgaacctct ggccgttcct tccgctgctg
     3661 ctgattcccc tggttcccgc gatcttcggc accggtgtca cgatcaccga tccggccgag
     3721 atcgcgctcc tcgtcctgat gaacttcacc gtcggcttcg gcgaggaggc gacgttccgc
     3781 ggagtcgtgc tgcgcgttct ggcgccgcgg ggcctcatgc gcgccgccgt cctctcgtcc
     3841 gtcctcttcg gcgcgatgca cctggtcaac ctcgccgtgg gcgagcaccc ggtgaatgtc
     3901 ggcttccaag tcatctacac cgtggagctc ggcttcgcct acgcctcggc cgcgctggtc
     3961 acgggcgcga tctggccgct ggttctgatt cacttcgccc aggatttcgc caacgctatc
     4021 caaacctcaa acaccggtag tggctcgccc ggcggcgttg atctcgccag cggcctgatc
     4081 aacgtggcgc tgtggttgcc cttcgtcgtg tacggctact ggctgctgcg ccatcacctg
     4141 cggcaatcgg gctccgagga tgagcgtccc ggcccgcgcg tgccggcgat gtcagcagag
     4201 gacgtgaccc ttggccgcgg gcgcgcgtgg cggtccgccg gatgacccgt gaggaggacg
     4261 cacgatgcac gctcaacgca tgcgcagcac gctgacgaag gacagtggat cacgggggga
     4321 tacaggcaga ccggggagcc ctgatcaacg catcagcctc aaggtcttct tcgccctggc
     4381 attcgggcta ggctggggga tcgctatcct catgctgctc ttccagacgc agatcgaggc
     4441 ggtcttcggc gagatcagcg gcaccaaccc ggtgttcatc ctggtggtct actcgccggc
     4501 catcgccggc atctatctgg tctggcgcca ctacggcgtc aaggccctgg gcagcttctt
     4561 ccggcgcctg acgatgtggc gtctaccaat ggcgtggtgg ctgttcgtgc tgctcggcat
     4621 cccggcggtg aaatacctcg gcgccgcctt caacggcacg gcgaccgagt tccccttctc
     4681 tccctggtac gacgtgctgc ccgccctggc agccgcgctt ctcattggcc cggtcgagga
     4741 gttcggctgg cgaggggtcg cgctgccgct gctccagcgc cgcttcgtgc cgctggcggc
     4801 cagcctcatc ctgggcgcct tctgggggct ctggcacctg ccagccttca tgttcagcgg
     4861 cacgcctcag agcgcgtggt cgttcggtcc attcttgatc ggggtgatgg cgctggcggt
     4921 cattctgact ccgatcttca acgccgcacg gggcagcatg ctgtttccaa tcctgtttca
     4981 cttcctgatc aacggaccga tgtggcccga agcccagccc tgggagaact tcatcttcgc
     5041 cgccctggcg gtagctatcg tggcgctgaa ctggcagacg atgacgacgc gtggaggggc
     5101 ggcgaccgag atcctgacgc cgggcgcaga agatgttggc gcgggagcgg ttgaaacacg
     5161 cacggcgatg acgcgtgcgg gggcctgatc gtctcgctgc gtcccagcgg cgccgcgtcg
     5221 ctcaactacg ttgggcggcg cggcaccgct ggacgtccgg caatcaggca gcctgaccac
     5281 ggaatagcgc ggcttcgagc cccattacct gctcgggcaa ccgcttagtt cgtcgaggcg
     5341 gtcgtcagcg ttcgcaggcg cgcggcgccc accagcggct cggcctgcca cggcacggtc
     5401 cagagtcggg atgccagcgt ggccgcgacc tcgtggacct ggcgtagctc ctggcgcgcg
     5461 cgctcggcca gcaggggatg cgccgttccg ctggtcgcga gcaactggtt gacgatccag
     5521 ccgtagggct cgatgccggc ccggcgcagg tcctcctgca gccgggctgc ctccagaacc
     5581 ggcgtgccct cgggcagcgt cacgatcagc acgtgcgcga agctcggatc ctgcaggcgc
     5641 atcagcggcg tggtcaggcg cccgggtagc tgaccggcct tgcgcacgat ctcgcggtgg
     5701 taggcgcccg tggcgtccag cagcagcagc gtgtgcccgg tcggggcggt gtcgagcacc
     5761 acgaagcgat tgcgggcctg ggcgatggtg cgtgagaagg cctggaagac ggcaatctcc
     5821 tcggtgcagg gcgagcgaag atcctcctcc agaagcgcca gcgcgtccgg ctcctgcccg
     5881 gcggcgcgcc gcagcacctc ttgccggtag cgcgcgctct cgacggccgg gtcgatacgg
     5941 ctgagcgtca ggttggccgg cagctcgccc ccgagcgcgt cgaggaggtg cgccgc
//
LOCUS       DASUBI010000571         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_245567, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000571 DASUBI010000000
VERSION     DASUBI010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245567"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFU72_11040"
     CDS             <1..607
                     /locus_tag="VFU72_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424067.1"
                     /translation="RTTANPVGTAFVTLSGGQQADVLCLAPWVPVFVNGGHECVLAEA
                     FHPSLDPLPGSPAFNVPTDRHVAQRNLSVVMAAKGMFHLAFEVHNPSRRARVFSLSIQ
                     EGKLDELKPLLATLGPTFKPPERQGRLGRLGFVRDACPAPDDLKDQQPRIDKLELRPG
                     ERAGFSLVGQLEGPGALVHVLQQADEQTVGGLSVLVRAEGE"
     gene            610..2145
                     /locus_tag="VFU72_11045"
     CDS             610..2145
                     /locus_tag="VFU72_11045"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEU5424068.1"
                     /translation="MAAITVPNRPFASDIITGLALPDGFFEASLGQQRINAQFQNGGA
                     AIGSIDLYVESVSHPGIVVTPRTYTVTNLAAGAARVLSWDADFSAAPPGTHYISFIAE
                     DGGGHTRVIKKIFVTRVQFDPATLTFWAEAPEGTIEVRLQDLVGPKGACCPVPKNPDR
                     VRSRAAEGQLLAQVARLFEGHRPDFPFCIPGYLLHEFDVAIAPNPPFAGQYSDLPYQD
                     PWWKVLLCIIALILLIAAAIAEATGGSGSVTVSTGSTDTGSPVGDCCGIRAGGGGTSY
                     LAAGLVAGAAAAATAAALSDVRDPIRRGQDNTMPVAGEITVGERLHATITYVEAVALG
                     RPFKVQADWEYTRVTTGNTYTYSVSETNSNEHVISKEEIDAPDVVRLYHPEPWIVRAQ
                     FFDADNNPLRGADLFVQCFLIGPHGEYLQFVLQDDGIAPDDKPDDGIYTGRFDFRVLE
                     EKARGLWQYYVIAQDVNNAQPGMSPEEAATIIGGLVRSHQLTITFDGGTCPLVPDGFV
                     NVI"
     gene            complement(2192..2731)
                     /locus_tag="VFU72_11050"
     CDS             complement(2192..2731)
                     /locus_tag="VFU72_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5424069.1"
                     /translation="MPLIMPFQGKTPRIAEDVFVAPSAVIIGDVEIGEGSSVWFGVVI
                     RGDIEAIRIGRRTNIQENVVIHADPGLPTTVGDEVTVGHGAIVHGCRVASRAQIGMGA
                     VLLNGASVGSGTLIGAGAVVPEGAEIPPGSVALGVPAKVRRESTQEERQGLLASAEHY
                     AERGRAMRAALEDEQSHWL"
     gene            complement(2751..>3376)
                     /locus_tag="VFU72_11055"
     CDS             complement(2751..>3376)
                     /locus_tag="VFU72_11055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5424070.1"
                     /translation="GKLGVRAGDTGWLSFNNLRVPVENRIGEEGEGFKIAMSAIDQGR
                     FTVAAGAVGTIRASTEASLKYAHERQAFGQEIGRYELVKQLIARMVAGEEASRLLVMR
                     SAELKNRGGRNTKETSLAKWVACNHAFEAANDAIEIHGAYGYSNEYPVERYLRNARGA
                     QLYEGTNEIHTIMQGDYALGYRVDRPLRCPQPPAQPFEEEAKQAAKA"
BASE COUNT          497 a         1236 c         1124 g          519 t
ORIGIN      
        1 ccgcaccacc gccaacccgg tcgggaccgc cttcgtgaca ctgagcggcg ggcagcaagc
       61 ggacgtgctc tgtctcgcgc cctgggtgcc ggtcttcgtc aacggcgggc acgagtgcgt
      121 gctggccgag gccttccacc cctcgctcga tccgctgccc ggcagcccgg cgttcaacgt
      181 gcccaccgac cggcacgtgg cccagcgcaa tctgagcgtg gtgatggccg ccaaaggcat
      241 gttccatctg gccttcgagg tacacaaccc gtcccgtcgc gcccgcgtct tcagcctcag
      301 cattcaggag gggaagctcg acgagctgaa gccgctgctg gcgacgctcg ggcccacctt
      361 caagccgccg gagcggcaag ggcgcctcgg ccggctcggc ttcgtgcgcg acgcctgccc
      421 ggcgccggac gatctcaagg accagcagcc gcgcatcgac aagctcgagc tgcgccctgg
      481 tgagcgcgcc ggcttcagtc tcgtcgggca actcgaaggc cccggcgcgc tggtccacgt
      541 gctccagcaa gccgacgagc agaccgtcgg tggcctgagc gtcctggttc gcgcggaagg
      601 ggaatgacga tggccgcgat caccgttcca aaccggccct tcgccagcga tatcatcacc
      661 ggcctggcgc tccccgatgg cttcttcgaa gcctcgctgg ggcagcagcg gatcaacgcc
      721 cagttccaga acggcggcgc cgccatcggc agcatcgacc tctacgtcga gagcgtgtcg
      781 cacccgggca tcgtcgtcac gccgcgcacc tacaccgtca ccaacctggc cgccggtgcg
      841 gcccgcgtgc tcagctggga cgccgacttc agcgccgcgc cgcccggcac ccactacatc
      901 agcttcatcg ccgaggatgg cggcggccac acgcgggtca tcaagaagat cttcgtcacc
      961 cgggtgcagt tcgatccggc caccctcacc ttctgggcgg aggcccccga gggtacgatc
     1021 gaggtccgcc tccaggacct cgtcgggccc aaaggcgctt gctgcccggt gcccaagaac
     1081 ccggataggg tccgctcgcg cgccgccgag gggcagttgc tggcgcaggt cgcgcgcctc
     1141 ttcgaaggcc accgccccga cttccccttc tgtatacccg gctacctgct gcacgagttc
     1201 gacgtcgcca tcgcgcccaa cccgcccttc gccgggcagt acagcgacct cccctaccag
     1261 gacccctggt ggaaggtgct cctctgtatc atcgcgctga tcctgctgat cgccgccgcg
     1321 atcgcggagg ccacgggcgg atcgggcagc gtcaccgtat cgacggggag caccgacacc
     1381 ggcagcccgg tcggcgactg ctgcggcatc cgggccggcg gcggcggcac cagctatctg
     1441 gccgccgggc tggtggccgg cgcggccgcc gcggccacgg cggcggcctt gagcgacgtg
     1501 cgcgacccga tccggcgcgg ccaggacaac accatgccgg tggccggcga gatcaccgtc
     1561 ggcgagcgcc tgcacgccac catcacctac gtcgaagcgg tggcgctggg gcggcccttc
     1621 aaggtccagg ccgactggga gtacacgcgc gtcaccaccg gcaacaccta cacctactcg
     1681 gtcagcgaga cgaacagcaa cgagcacgtc atctcgaagg aggagatcga cgcgccggat
     1741 gtcgtgcgcc tctaccaccc cgagccctgg atcgtgcgcg cccagttctt cgacgccgac
     1801 aacaacccgc tgcgcggcgc cgacctcttc gtgcagtgct tcctgatcgg cccgcacggg
     1861 gagtacttgc agttcgtgct gcaagacgac ggcatcgccc cggacgacaa gccggacgat
     1921 gggatctaca ctggacggtt cgacttccgc gtgctggagg agaaagcgcg cggcctgtgg
     1981 cagtactacg tcatcgccca ggacgtgaac aacgcgcagc cggggatgag cccggaggag
     2041 gcggccacga tcatcggcgg gctggtgcgc agccaccagt tgaccatcac cttcgatggc
     2101 ggcacttgcc cgctggtgcc ggatggcttc gtcaacgtga tctgagcggg aacctgcgcg
     2161 gccggcgcgt ctttccgccg gtcgcgcgcc gctaaagcca gtgggactgc tcgtcctcca
     2221 gcgccgcgcg catggcgcgc ccgcgctcgg cgtagtgctc ggcgctggcc agcagcccct
     2281 gacgctcctc ctgggtggac tctcgtcgca ctttggccgg cacgccgagg gccaccgagc
     2341 cgggcggaat ctcggccccc tccggcacca ccgctccggc cccgatcagc gtgccgctgc
     2401 cgacgctggc gccgttgagc agcaccgcgc ccatgccgat ctgggcgcgc gatgccactc
     2461 ggcagccgtg gacgatggcg ccatgcccca cggtgacctc gtcgcccacg gtcgtcggca
     2521 ggccaggatc ggcgtggatg accacgttct cctggatgtt ggtgcgccgg ccgatgcgga
     2581 tcgcctcgat atcgccgcga atgaccacac cgaaccagac gctggagccc tcgccgatct
     2641 cgacgtcgcc gatgatcacg gcgctcggag cgacgaagac gtcctcggcg atgcgcggcg
     2701 tcttgccctg aaagggcatg atcaacggca ccggcggctc cctcctggcc tcatgccttg
     2761 gccgcctgct tggcctcctc ctcgaagggc tgcgccggcg gctgcgggca gcgcagcggg
     2821 cggtcgacgc ggtagccgag ggcgtagtcg ccctgcatga tggtgtggat ctcgttggtg
     2881 ccctcgtaga gctgcgcgcc gcgcgcgttg cgcaggtagc gctcgaccgg gtactcgttg
     2941 gagtagccgt aggcgccgtg gatctcgatg gcgtcgttgg cggcctcgaa ggcgtggttg
     3001 caggcgaccc acttggccag cgaggtctcc ttggtgttgc ggccgccgcg gttcttcagc
     3061 tcggccgagc gcatcaccaa gaggcgcgag gcctcctcgc cggccaccat cctggcgatc
     3121 aactgcttga ccagctcgta gcggccgatc tcttgcccga acgcctggcg ctcgtgggcg
     3181 tacttcaggc tggcctctgt gctggcgcgg atggtgccga ccgcgcccgc tgccacggtg
     3241 aagcgcccct ggtcgatggc gctcatggcg atcttgaagc cctcgccctc ctcgccgatg
     3301 cggttctcga ccggcacgcg caggttgttg aaggagagcc agccggtatc gccggcgcgc
     3361 acgccaagct tgccgt
//
LOCUS       DASUBI010000572         6486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_219705, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000572 DASUBI010000000
VERSION     DASUBI010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219705"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="lepB"
                     /locus_tag="VFU72_11060"
     CDS             <1..571
                     /gene="lepB"
                     /locus_tag="VFU72_11060"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5424071.1"
                     /translation="LLAVFIFIAVRSIILNYRVDGSSMLPNLHDHEMLVVNRRAYFHM
                     NLNSLINWLPGVHRDGQLIWYPFDPPARGDIVIFNPPGQHTEPYIKRIIGLPGDHVTI
                     HDGGVYVNGTRLDEGYLNSDTAWRGTSAEYDVPAGHIFVLGDNRNNSSDSRVFGPVPV
                     SSVIGKAWIAYWPPSEAHVLHDPNYSASP"
     gene            708..1241
                     /locus_tag="VFU72_11065"
     CDS             708..1241
                     /locus_tag="VFU72_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5424072.1"
                     /translation="MIETVVESIRVSLVTQHRVVILKEVGGDRHLPIWIGPFEAEAIA
                     MELQSMSSARPLPYDLMRTIITDMGGTVRQVLVTDLSNDVFYARIVLDHDGQEIEVDS
                     RPSDAIALAVRVKVPILVEETVMDRAGVSLEHESDEEEAPEREAVKGEEATGDLSVFR
                     EFINSLDLDDFEKKRDN"
     gene            1254..1607
                     /locus_tag="VFU72_11070"
     CDS             1254..1607
                     /locus_tag="VFU72_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424073.1"
                     /translation="MEPDNIDVRRTVLDVLGDCSQCQSSYQLDDARIVGRTGNLWVLA
                     IRCSRCDAQAFVAAVVGDHDDDEALEYLDDEVLSEVVAEYTTSPVTVDDVLDMHEFLD
                     AFDGDFLSLFSRRSR"
     gene            1676..2260
                     /locus_tag="VFU72_11075"
     CDS             1676..2260
                     /locus_tag="VFU72_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5424074.1"
                     /translation="MAALVPHPPEVRAALESEALGQIDALYRTAVRLTRNPADAEDLV
                     QETYLRAFRGLDRFQPGTNLRAWLFKILTNAFINEYRRRSRQPKSTSLDDTEEYYVYA
                     HLIDSGIQPSSRRPEDEVLASITDESVVRALEALPEEFRQVVLLADVEGFAYREIAEI
                     MDIPIGTVMSRLHRARRRLQRSLVESYVGDVPHD"
     gene            2253..2492
                     /gene="rsrA"
                     /locus_tag="VFU72_11080"
     CDS             2253..2492
                     /gene="rsrA"
                     /locus_tag="VFU72_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEU5424075.1"
                     /translation="MTNCDDAVKRLYEFLDRELSPEELAEVQRHLDACPPCRDRFTFE
                     ADVLRLVRKSCRDVSAPPELVERVKRICNQERQTR"
     gene            complement(2480..2911)
                     /locus_tag="VFU72_11085"
     CDS             complement(2480..2911)
                     /locus_tag="VFU72_11085"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEU5424076.1"
                     /translation="MWWWDNLIEDKIRTAQEAGQFDNLRGAGRPFQWNDDSRDEDWLG
                     NHLLHNAGVLPEWLQLRKEIAIERPSVVEALDTYWRQREKLDPGDRRQAALLARLEAE
                     YAARAREINRKIDLHNLRCPSLAHEMPRYPEDLIRRRLQRV"
     gene            3053..3595
                     /locus_tag="VFU72_11090"
     CDS             3053..3595
                     /locus_tag="VFU72_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5424077.1"
                     /translation="MVHTTEPNAEIDLQALREILQRDFQPGDYQEAQELIVGACQEAQ
                     SMYGWVSPDAAQLIADHLGVTINRIYGLLTFYADFRTEPPGKHAMLLCYGAACFVMGA
                     DKLVQSIRREPGLDADGRTSDGEFSVQIVNGCLGVCNLAPVVQIDHHHYFGHLTPEKL
                     QSAMQAVARGESPEDADAAE"
     gene            3582..5315
                     /locus_tag="VFU72_11095"
     CDS             3582..5315
                     /locus_tag="VFU72_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5424078.1"
                     /translation="MPLNSRADFEAYAAKAKDAWQEREQSDRWQVKVPVSECSIAQGA
                     METIEHLRLVLEQAGVQFDLRRVGCSGWCWAEPWVEVRVPGHPPIVYMNITTDRVPEL
                     IAGLRRGDLQPDLALGVDASEPYEGIQPLSSHPYIAPQRRLLLENAGVIDPESIEEYI
                     ARGGYRAWTKALFDMTPDQVVNEIKTANVRGRGGAGFPAGIKWESGRRTPSWPKYVIA
                     NSHEGEPNVYKDRRLIETDPHLVLEGIMLGCYALETPYGYNYVGGEHVLAVKRFRTAV
                     QQAYELGLLGDNVLGTGIACHVRTRTGGGAYICGEGSALMYSVMGQRGQPRTKPPRSV
                     EEGVWRRPTVLNNTETFANAPAIVLNGGEWYAAIGTEKSKGTKLVTIQGPVKRIGLVE
                     VEMGLPMRELIFGIFGGMADGYTFKGVQTGGVSAGPLKEDQLDLLVDFDSMTEAGGML
                     GSGGFVVFDETVCAVDFARYLMQFNRYESCGKCTPCRLGTPALLEILDRMRNGKARPG
                     DLELIDGSSKHVIDLSLCGLGQVAPMPVLGMKRAFPEEFDAHVREGRCPAGACPCAQG
                     ETTLSLAGAGQ"
     gene            complement(5793..>6486)
                     /locus_tag="VFU72_11100"
     CDS             complement(5793..>6486)
                     /locus_tag="VFU72_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5424079.1"
                     /translation="NQQAAFLRDRELGRALARMFTAYLTEELQHYREQGDGAPASVTT
                     RLMRLTVDGKLLTDVEIVSIVRNWTAGVGTVAAGIGILLLHLATRQDLQQRLRAEPSV
                     LPQAVDEILRVDGPLVANRRTTTREVEIDGRQIGQGQRISLMWIPADRDPRAFDDPED
                     VRLDRNEQGTVLFGDGIHYCQGAPLARLQMRIALEELLRQSRHIELSGAEPPSRVTFP
                     SNGMLTLSLRIS"
BASE COUNT         1151 a         2024 c         2177 g         1134 t
ORIGIN      
        1 gctgctggcc gtcttcatct tcatcgccgt gcgcagcatc atcctgaact accgcgtgga
       61 cggcagcagc atgttgccga atctgcacga ccacgaaatg ctggtggtca accggcgtgc
      121 gtatttccat atgaatttga actcactgat caactggctg cctggagtcc atcgcgacgg
      181 ccagctcatc tggtatccct tcgatccccc cgcgcggggc gacatcgtca ttttcaaccc
      241 gccggggcag cacacggagc cgtatatcaa gcgcatcatt ggcctgccgg gggatcacgt
      301 cacgattcac gacggcgggg tctacgtcaa cggcacgcgt cttgacgagg gctacctcaa
      361 ctcggatacg gcctggcgag ggacttcggc ggagtacgat gtgcccgcgg gacacatctt
      421 cgtgctcggt gacaaccgca acaatagtag cgactcgcgc gtcttcgggc cggtgccggt
      481 ctcctcggtc atcggcaaag cctggatcgc ctattggcca ccaagcgagg cgcacgtctt
      541 gcacgatcca aactactcgg cgtcgccgta ggatgtcgtc cacgtcctag cggtgtcagc
      601 aggtcggcag tagaatgaaa agtgacgtgc cgtacggcgc aggtagcacg agccgcggct
      661 gaggtccgcg ttgatacgca ccagcctgct acaggaaggg gcgttccatg atcgaaaccg
      721 tggtcgagag catccgggtg agtctggtga ctcagcaccg ggtggttatc ctgaaggaag
      781 tgggcggcga tcgtcacctg ccgatttgga ttggcccgtt cgaagccgaa gcaatcgcga
      841 tggagctcca atcgatgtcg tcggcgcggc cactgccgta cgacctgatg cggacgatca
      901 ttaccgatat gggtggcacc gtgcgccagg tgctggtgac ggatctttcc aatgatgtct
      961 tctacgcccg catcgtcctc gaccacgacg ggcaggagat cgaggtcgat agccggccga
     1021 gcgatgcgat cgcgctcgcg gttcgggtca aggtgccgat cctggtcgaa gagacggtca
     1081 tggaccgcgc cggagtcagc ctggagcacg agagcgacga ggaagaggcg ccggagcgcg
     1141 aggcggtgaa gggcgaggaa gcgacgggcg acctgagcgt cttccgtgaa ttcatcaact
     1201 cacttgatct ggacgacttt gaaaagaagc gagacaacta gaagcacgac atgatggaac
     1261 ctgacaacat cgacgtccgg cgtacggtcc tcgacgtgct gggcgactgt tcacagtgcc
     1321 aaagcagcta tcagctcgac gacgcgcgca tcgtcgggcg cacgggcaat ctatgggtgc
     1381 tcgcgatccg ctgctcgcgc tgcgacgcgc aagcgttcgt ggctgcggtc gtcggcgatc
     1441 acgacgacga tgaggcgctc gaatatctgg acgacgaagt actctcggaa gtggtggcgg
     1501 agtacacgac gtcaccggtg acggtggacg acgtgctcga catgcacgaa ttcttggacg
     1561 cgttcgacgg ggactttctc tccttattca gccgtcgttc ccgttagcca tgcgagacgc
     1621 gcgattgatc ccagtccggg tgcgacgtgg cgctgggtga ggggagacgc tggtagtggc
     1681 tgctctggta ccacatccgc cggaggtgcg cgcggcactc gagagcgaag ccttgggaca
     1741 gatcgacgcc ttgtatcgca cggctgtccg tttgacccgc aacccggctg atgccgagga
     1801 tctcgtgcag gagacgtatc tgcgggcttt ccgtggcctg gatcgctttc agccgggcac
     1861 aaacttgcgt gcctggttgt tcaagattct gacgaacgcg ttcatcaacg agtatcgtcg
     1921 caggtcgcgg caaccgaaga gcacgtcgct cgacgacacc gaggagtact acgtgtacgc
     1981 ccacctgatc gattcgggca tccagccctc gtctcgccgg ccggaggacg aggtgctcgc
     2041 gagcatcacc gacgaatcgg tggtgcgggc gctggaggcg ctgccagaag agttccgtca
     2101 agtagtactc ttggccgatg tcgaaggctt cgcgtatcgc gagatcgccg agatcatgga
     2161 tatcccgatc ggcaccgtca tgtcgcgcct gcaccgggcg cgccgtcggc tgcaacggtc
     2221 gctggtggag agctacgtcg gggacgttcc acatgactaa ctgcgatgat gccgtcaaac
     2281 ggctctatga gtttctcgac cgggagctgt caccggagga gctggcggag gtccagcgtc
     2341 atctggatgc ctgccctccc tgccgcgatc gcttcacctt tgaggcggac gtgctgcgcc
     2401 tggtgcgcaa gagctgccgt gacgtttccg ccccgccaga gctggtcgag cgtgtcaagc
     2461 gcatctgcaa ccaggagcgt cagacgcgct gaagccggcg gcggatcaga tcctcgggat
     2521 agcgcggcat ctcgtgggcc agcgaggggc agcgcaggtt gtgcagatcg atcttgcggt
     2581 tgatctcacg agcgcgggcg gcatactcgg cttccagccg cgccaacagc gcggcctgtc
     2641 gccgatcacc ggggtctagc ttctcccgct ggcgccagta ggtgtcgagc gcttctacga
     2701 cagagggccg ctcgatcgcg atctccttac gcagttgcag ccactcgggc agaacccccg
     2761 cgttgtgcag cagatggttg cccagccaat cctcgtccct ggagtcgtcg ttccattgga
     2821 atggacggcc cgcgccgcgc aggttgtcga attgccccgc ttcctgagcg gtgcgtatct
     2881 tgtcttcgat caggttatcc caccaccaca cgcgcactcc cgccgctcgg cccgctcgtt
     2941 tccaagcccc aagtgtagca ggagagcgct ttgctatact cggtcggtaa cgggtgcggc
     3001 tggtgaggcc gggggtggcc cacgtccagt cgctcagacg agagggcgcg acgtggttca
     3061 cacgacggag ccgaacgccg agattgacct ccaagccctg cgggagattc tgcagcgaga
     3121 cttccaacca ggcgactatc aagaggcaca ggagctgatc gtcggcgcct gccaggaggc
     3181 gcagtcgatg tacggctggg tttcccctga cgcggcccag ttgatcgcgg atcacctggg
     3241 cgtgacgatc aaccgcatct atggcttgct gaccttctat gccgatttcc gcaccgagcc
     3301 gcccgggaag cacgcgatgc tcctctgcta cggcgcggcc tgcttcgtga tgggggcgga
     3361 caaattggtg cagtcgatcc gaagagaacc tggtctggat gccgacggcc gcacctcgga
     3421 cggtgagttc tcggtccaga tcgtgaacgg ctgcctgggt gtctgcaacc tggcgccggt
     3481 ggtccagatc gatcatcatc actatttcgg ccacctgacg cccgaaaagc tccagtcggc
     3541 catgcaggcg gtcgcgcggg gcgagtcacc ggaggacgcc gatgccgctg aatagtcgag
     3601 cagacttcga agcctacgcc gccaaggcca aggatgcctg gcaggaacgc gagcagagcg
     3661 accgctggca ggtgaaagtg ccggtcagcg agtgcagcat cgcgcagggg gcgatggaga
     3721 cgatcgagca cctgcgtctg gtgctggagc aggcaggggt gcagtttgac ctgcgccggg
     3781 tcggttgctc cggttggtgc tgggcggagc cgtgggtcga agttcgcgtg cccggccacc
     3841 cgccgatcgt ctacatgaat atcacgaccg atcgcgtgcc ggagctgatc gccggcctgc
     3901 gccgtggcga cctgcaaccc gacctggcgc tcggagtcga cgccagcgag ccgtacgagg
     3961 gcatccagcc gctcagcagc cacccgtaca tcgcgccgca gcgacgcctc ctgctggaga
     4021 acgccggggt gatcgaccct gagtcgatcg aggagtacat cgcgcgcggc ggctaccggg
     4081 cctggaccaa ggcgctcttc gacatgacgc cggatcaggt cgtcaacgag atcaagacgg
     4141 ccaacgtgcg ggggcgcggc ggggccggct tcccggcggg catcaagtgg gagagcgggc
     4201 ggcgcacgcc gtcctggccc aaatatgtga tcgccaacag ccacgagggc gagccgaacg
     4261 tctacaagga ccgtcgtctg atcgagaccg atccccacct ggtgctggag gggatcatgc
     4321 tcggctgcta tgccctggag acgccctacg gctacaacta cgtcggcggc gagcatgtgt
     4381 tggcggtcaa gcgcttccgt accgcggtcc agcaggctta cgagcttggg ttgctgggcg
     4441 acaacgtgct cggcaccggc atcgcctgcc acgtacgcac gcggaccggc ggcggcgcgt
     4501 acatctgcgg cgagggctcg gcgctcatgt actcggtaat gggccagcgc ggccagccgc
     4561 gcaccaagcc gccgcgctcg gtcgaagagg gcgtctggcg ccggccgacc gtgctgaata
     4621 acaccgagac ctttgccaac gcgcccgcga tcgtgctcaa tggcggcgaa tggtacgccg
     4681 cgatcggcac cgagaagagc aagggcacca agctggtcac gattcagggg ccggtcaagc
     4741 gcatcgggtt ggtcgaggtc gagatgggcc tgccgatgcg cgagctgatc ttcggcatct
     4801 tcggcggcat ggccgacggc tacaccttca agggggtgca gaccggcggc gtgtcggccg
     4861 gaccgctcaa agaggatcag ctcgatctgc tggtcgactt cgattcgatg accgaggccg
     4921 gtggcatgct cggctcgggc ggcttcgtcg ttttcgacga aacggtctgc gcggtcgact
     4981 tcgcgcgcta cctgatgcag ttcaatcgct acgagtcgtg cggcaagtgc accccctgcc
     5041 gccttggcac ccccgcgctg ctggagatcc tcgaccgcat gcgtaacggc aaggcgcgac
     5101 ctggcgacct tgaactgatc gacggcagca gcaagcacgt catcgatctg tcgctctgcg
     5161 gcttgggaca ggtggcgccg atgccggtac tggggatgaa gcgcgccttt cccgaggagt
     5221 tcgatgcgca tgtgcgcgag ggccgctgcc cggcaggcgc gtgcccctgc gctcaggggg
     5281 aaacgacgct gtcgctggcg ggcgccggcc agtagccgct gcggctggac gtgcaccttg
     5341 gcagtctgaa gatcagacgc gtgccgaggg cggaagcgtc gcttacgctg cacagaccgg
     5401 ggccgaagcc gcggacaagg cagttcgtgc ggcaggcgaa cgcatccttc gccaactcgg
     5461 tcgacgacgg cggctgcaac ggggaatccg cggcttcggc gtgccgagtc ggtggtttcc
     5521 agagcgcggc cgccgaggct atgcacggcc gggcgaagat gcgcgccagg cgcaacccgc
     5581 gcgacgacga gtagaggacc actggcgccg tgtggtgccc attcgacaat ctggtgttca
     5641 acgcccgagt gcctcctgat ccggcgtgtt atctaccgca cgcgtgcacg cgcgatggtg
     5701 cgagcatcga ggtccgaccg cggttgttgc actgaactgc cggaccgggt ggggcgcacg
     5761 accacgcaaa ggcggccgcg ggcacgtcct ggttagctga tccgcagcga aagcgtcaac
     5821 atcccgttgc tcgggaacgt cacccggctc ggcggctcgg cgcccgacag ttcgatgtgc
     5881 ctactttgtc gcagcagctc ctcgagcgcg attcgcatct gcaggcgggc cagcggcgca
     5941 ccctggcagt agtgaatacc atcgccaaag agcacggtgc cctgctcgtt ccggtccagc
     6001 cgcacatcct cgggatcatc gaaagcgcgc gggtcgcggt cggcgggaat ccacatcagg
     6061 gagatgcgct ggccctgtcc aatttgcctc ccgtcgatct cgacctcacg cgtagtcgtg
     6121 cggcggttgg cgactaaggg gccatccacg cgcagaatct cgtcgacggc ctgtggcagc
     6181 acgctgggct cagcacgcag ccgctgctgc agatcctgtc tcgtggccag atgaagcagc
     6241 agaatgccga tgccggcggc aaccgtgcca acgccagcgg tccaattgcg cacgatgctg
     6301 acgatctcga cgtcggtgag cagcttgcca tcgaccgtca ggcgcatcag ccgggtggtc
     6361 acgctagcgg gcgcgccgtc gccttgttcc cggtaatgct ggagctcctc ggtcaggtac
     6421 gcggtgaaca tgcgcgccag cgcgcgaccc aactctcgat cgcgcagaaa cgccgcctgt
     6481 tggttg
//
LOCUS       DASUBI010000573         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_65036, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000573 DASUBI010000000
VERSION     DASUBI010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65036"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFU72_11105"
     CDS             complement(<1..1083)
                     /locus_tag="VFU72_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5424080.1"
                     /translation="MKRNTVTSERPPVPAQPAPNSEPTTYLSHDQILVVLIGLMAGML
                     LAALDQSIVSTALPRIVSDLGGLDKLSWVITAYLLTSTAATPLWGKISDLYGRRLIFQ
                     IAIGVFLIGSALSGLSQNMVELILFRAVQGIGGGGLMAIAFAIVGDIIPPRERGRYQG
                     YFGAVWGVASVAGPLLGGWFTDGPGWRWIFYINLPIGLLALGITSVVLKMPAVKRDHA
                     IDYLGATAIVAGVTALLLYLNWAGEAFGWFAPGPLALVATFVLLSAAFVWIEYRASEP
                     IIPLRLFRDDIFSVSNIYGFLAGVAMFGAIIFLPLYLQAVEGMSPTRSGLAMLPAVTG
                     IFTMSILSGYLITRTGKYKIYPILGAA"
     gene            complement(1189..1647)
                     /locus_tag="VFU72_11110"
     CDS             complement(1189..1647)
                     /locus_tag="VFU72_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU5424081.1"
                     /translation="MAPRDTPEAARQRDERFRRTSSEHLQRYLATGGDEGYISPESGM
                     PNLILTTIGRRTGEPYATPLYYGEDEGRYILVASYAGADKHPKWYLNLVANPAVEVQI
                     RGERFAARARTATPQEKPALWAMMAARYPRYDVFQQDTEREIPVIILERV"
     gene            complement(1713..>2331)
                     /locus_tag="VFU72_11115"
     CDS             complement(1713..>2331)
                     /locus_tag="VFU72_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424082.1"
                     /translation="WLALARSGVLWHHTAATLQEALAGFLVAAAIAIALGYVLARSEA
                     LMSVLAPYIALTQAVPILAFAPLLVVWFGLGLLPKVIICVLIVFFPMLINTIVGLRSI
                     DQAMLLAARGLGASRWRVLWRIEVPLALRTLLGGVKMGITLSMAGAVVGEFVASDAGL
                     GYLMNFGRTAYDTPTVFAASLTMAALGTIGFVAVSVLERMLIRWE"
BASE COUNT          413 a          749 c          837 g          332 t
ORIGIN      
        1 ggccgcgccc aggatcgggt agatcttgta cttgccggtg cgcgtgatca ggtagccgga
       61 gaggatcgac atggtgaaga tgccggtcac cgccggcagc atcgccagcc ccgagcgcgt
      121 gggcgacatg ccctcgaccg cctgcagata gagcggcagg aagatgatgg cgccgaacat
      181 ggccacgccg gccaggaagc cgtagatgtt gctgacgctg aagatgtcgt cgcggaagag
      241 gcgcagcggg atgatcggct cgctggcgcg gtactcgatc cagacgaagg ccgcgctgag
      301 cagcacgaag gtggcgacca gcgccagcgg gccgggcgcg aaccagccga aggcctcgcc
      361 ggcccagttc aggtagagca gcagcgccgt gacgccggcc acgatggcgg tcgcgccgag
      421 gtagtcgatg gcgtggtcgc gcttgaccgc cggcatcttg agcaccaccg aggtgatgcc
      481 cagcgccagc aggccgatcg gcaggttgat gtagaagatc cagcgccagc cggggccatc
      541 ggtgaaccag ccgcccaaga gcgggccagc cacgctggct acgccccaga ccgcgccgaa
      601 gtagccttgg tagcggccgc gctcgcgcgg cggaatgatg tcgcccacga tggcgaaggc
      661 gatcgccatc aggccgccgc cgccgatccc ttgcacggca cggaaaagga tcagttcgac
      721 catgttctga ctcaggccgg agagcgccga gccgatcaga aagacgccga tcgcgatctg
      781 gaagatcagc cggcggccgt agaggtcgga gatcttgccc cacagcggcg tggcggcggt
      841 cgaggtcagc aggtaggccg tgatcaccca ggagagtttg tccagcccgc ccaggtcgct
      901 gacgatgcgc ggcagcgccg tgctgacgat gctctggtcg agcgcggcca gcagcatgcc
      961 ggccatgagc ccgatcagca ccaccaggat ctggtcgtgc gagaggtagg tggttggctc
     1021 actgttcgga gcgggctggg cagggaccgg cggccgctcg ctggtaaccg tattccgttt
     1081 cattcagact ccatctgccg ggtgtcgcgc gtgatttcgg tgccgaccgc cccaagaatt
     1141 agagcggatc gaagtatagc gatttggacc atggaatgcc ggggtaactc agactcgctc
     1201 caggatgatc accggaatct cgcgctcggt gtcctgctgg aagacgtcgt agcgcgggta
     1261 gcgcgccgcc atcatggccc agagcgcggg cttctcttgg ggcgtggccg tgcgggcgcg
     1321 ggcggcgaag cgctcgccgc ggatctgtac ctcgacggcg gggttggcca ccagattcag
     1381 ataccacttg gggtgcttgt cggcgccggc gtacgaggcg accaggatgt agcgcccctc
     1441 gtcctcgccg tagtagagcg gggtggcgta gggctcgccc gtgcggcgcc cgatggtcgt
     1501 caggatgaga ttgggcatgc ccgactcggg gctgatatag ccctcgtcgc cgccggtcgc
     1561 caggtagcgc tgcagatgct ccgacgaggt gcggcggaag cgctcgtcgc gctggcgcgc
     1621 ggcctccggc gtgtccctgg gtgccacgtg ctggctcctt cctctcgaac gtcagccccg
     1681 gtgatggtgg agaacaatgc gccgggcctg tttcattccc agcggatgag catgcgctcc
     1741 agcacgctga cggctacgaa gccgatggtg cccaaggccg ccatcgtcag cgaggcggcg
     1801 aagacagtgg gggtgtcgta ggccgtgcgg ccgaaattca tcaggtagcc gagcccggcg
     1861 tccgaggcga cgaactcgcc gaccaccgcg ccggccatgg agagcgtgat ccccatcttg
     1921 accccgccga gcaacgtgcg cagcgccagc ggcacctcga tacgccagag cacgcgccag
     1981 cgtgaggcgc ccagcccgcg cgccgccagc agcatggcct ggtcgatcga gcgcagcccg
     2041 acgatggtgt tgatcagcat cgggaagaag acgatcagca cacagatgat gactttcggc
     2101 agcagcccca gcccgaacca gacgaccagc agcggggcga aggccaggat cggcaccgcc
     2161 tgggtcaagg cgatgtaggg cgccagcacc gacatcagcg cctcggagcg cgccagcacg
     2221 tagccgagcg cgatggcgat ggcggccgcc accaggaagc cggccagcgc ctcctgcagc
     2281 gtggccgcgg tgtggtgcca gagcacgccg ctgcgcgcca gcgcgagcca g
//
LOCUS       DASUBI010000574         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_715722, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000574 DASUBI010000000
VERSION     DASUBI010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715722"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFU72_11120"
     CDS             <1..428
                     /locus_tag="VFU72_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424083.1"
                     /translation="WQDKLAYRSIRPAASGWLRPRAGGIRFSTGRAVQAAEPQGGGLR
                     LRLDDGSARDVEHALLATGYRVDVRRYDFLAPELAGVVRQVNGYPVLGPGFESSVPGL
                     HFIGAPAAYSFGPVVRFVSGTWFTSRELARVVTRKVGAA"
     gene            428..1657
                     /locus_tag="VFU72_11125"
     CDS             428..1657
                     /locus_tag="VFU72_11125"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5424084.1"
                     /translation="MEARLEARGATATLARESPLPAPGGALIIGGDYRGLGVVRSLGR
                     AGVPVWVLTDEHRLAAVSRYSRRDLPFPVAADDETQAAFLLDLAGRSGLRGWALFPTG
                     DETAALIARHHDVLAAHYALTTPPWPVLRWTYDKRLTHELAATVGVASPWTGYPASRA
                     EVAALACDFPVILKPAAKPVANALTMVKAWRVDSREELLARYDEACRLLPPELLMVQE
                     LIPGGDDCQFSYAALCVEGRPLVSITVRRARQWPMDFGRASTFVETIAEPEVAGAARG
                     LIAAMGYQGLVELEFMRDPRSGVLKLIDINPRVWGWHSLGRRAGVDFPALQWRLLRGE
                     AVAERQAAAGVRWVRMTTDLPTVAREILGRRLSPLRYLWSLRRPIEFAIWAIDDPMPA
                     AAELPLIVRLARARGSL"
     gene            1801..2778
                     /locus_tag="VFU72_11130"
     CDS             1801..2778
                     /locus_tag="VFU72_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEU5424085.1"
                     /translation="MNGERIHERVATIGGGTGSFTMLSELKRFTTRIYAVVSMADSGG
                     SSRRLIDEFGRPLPLGDLRQALVALSRSSRLWGDIFSYRFPDSDSGVVGGHSLGNLIL
                     HALQDQNEGDLLGALEDAEEILNTAGHVLPVTVDNITLCAELSDGSLVVGESAIDRPE
                     SRAVLPIRRVFLDPAARATPRARKALERAEKILIGPGDLYTSVIPCLLVEGVPEAIRS
                     SDGEVIYICNVMTKHGETDGFAASDFVREIHHYLGRRVDAVVVNTGSLPPELVERYAR
                     QGAAPVAADLDVLRTLVPRVLTGSVATSERLIRHDAERVLMTIWPELNT"
BASE COUNT          456 a         1206 c         1194 g          538 t
ORIGIN      
        1 gctggcagga caagctcgcc tatcgctcga tccgcccggc ggcctccggc tggctgcggc
       61 cgcgcgcggg cggcatccgc ttctcgaccg ggcgcgcggt gcaagcggcc gaaccccaag
      121 gaggcgggct gcggctgagg ctcgacgacg gcagcgcgcg cgacgtcgag cacgcgctcc
      181 tggcgaccgg ctaccgggtc gacgtgcggc gttacgactt cctggcgccg gagctggccg
      241 gggtggtccg gcaggtcaac ggctacccgg ttctcgggcc gggcttcgaa tcctcggtgc
      301 ccggcctgca cttcatcggc gcgcccgccg cctatagctt cgggccggtg gtgcgcttcg
      361 tctcgggcac ctggttcacc agccgcgagc tggcccgcgt cgtcacgcgg aaggttggag
      421 cagcctgatg gaagcgcggc tggaggcgcg aggggcaact gccacgctgg cgcgggagag
      481 cccgctcccc gcgccgggcg gcgcgctgat catcggtggc gactaccggg gactgggggt
      541 ggtgcgcagc ctgggccggg ccggcgtgcc ggtctgggtg ctgaccgacg agcaccgcct
      601 ggcggccgtc tcgcgctact cccgccgcga cctgcccttc ccggtggccg cggacgatga
      661 gacgcaggct gcctttctgc tcgatcttgc cgggcgctcc ggcctgcgcg gctgggcgct
      721 cttcccgacc ggcgacgaga ccgccgcgct gatcgcccgg caccatgacg tgctggccgc
      781 gcactacgcg ctgacgacgc ccccctggcc ggtgctgcgc tggacctacg acaagcgact
      841 gacgcacgaa ttggccgcca cggtgggcgt ggccagcccc tggacgggct acccggccag
      901 ccgcgccgag gtcgcggcgc tggcctgcga cttcccggtg atcctcaagc cggctgccaa
      961 gccggtggcc aacgcgctca cgatggtcaa ggcctggcgc gtcgacagcc gcgaggagct
     1021 gctggcgcgc tacgacgagg cctgccggct gctgccgccg gagctgctga tggtgcagga
     1081 gctgatcccc ggcggtgacg actgccagtt ctcctacgcc gcgctctgcg tcgagggccg
     1141 cccactggtc tcgatcacgg tgcggcgcgc gcgccagtgg ccgatggact tcggccgggc
     1201 cagcaccttt gtcgagacga tcgcggagcc ggaggtggcc ggcgccgcac gcggcttgat
     1261 cgcggccatg ggctaccagg gcctggtcga gctggagttc atgcgggacc cacggagcgg
     1321 cgtgctcaag ctgatcgata tcaacccgcg cgtctggggc tggcacagcc tgggacggcg
     1381 ggcgggtgtc gattttccgg cgctccagtg gcgtctcttg cggggcgagg cggtggccga
     1441 gcgacaggcc gccgccggcg tccgttgggt ccggatgacg accgatctgc cgaccgtcgc
     1501 gcgggagata ctgggccgcc ggctgtcacc gctccggtat ctctggtcgc tgcgccggcc
     1561 gatcgagttc gccatctggg ccatcgacga tccaatgccg gccgcggccg agctgccgtt
     1621 gatcgtgcgc ctggcccggg cgcgtggttc actgtagggc ggggcgcgcc ctcatccccc
     1681 caaccccctt ctcccaatgc tgggagacgg gggagaaggt ttcgacgatg ctttggcgtg
     1741 aacctctctc ccagcattgg gagaaggggg ttgggggtga gggcccgtac gggggcaggg
     1801 gtgaacgggg aacgcattca cgagcgcgtg gcgacgatcg gcggaggcac cggctccttc
     1861 actatgctct cggagctgaa gcgcttcacc acgcgcatct atgccgtcgt ctcgatggct
     1921 gactccggcg gctcctcgcg ccggctgatc gacgagttcg ggcgcccgct gccgctcggc
     1981 gacctgcgcc aggcgctggt ggcgctctcg cgctccagcc gcctgtgggg cgacatcttc
     2041 agctaccgct tccccgactc cgacagcggc gtggtcggcg gccactcgct tggcaatctc
     2101 attctccacg cgttgcagga ccagaacgag ggcgacctgc tgggggcgct ggaggacgcc
     2161 gaagagattc tgaacacggc gggacacgtc ctgccggtga cggtcgacaa catcaccctc
     2221 tgcgccgagc tgtccgacgg ttcgctggtg gtgggtgagt cggccatcga ccggcccgag
     2281 agccgcgcgg tgctgccgat ccggcgcgtc ttcctcgacc ccgccgcccg cgccacgccg
     2341 cgcgcccgta aggcgctgga gcgcgccgag aagatcctga tcggccccgg cgacctctac
     2401 accagcgtca tcccctgcct gctggtggag ggcgtgcccg aggcgatccg ctcctccgac
     2461 ggcgaggtga tctacatctg caacgtgatg accaagcacg gcgagaccga cggcttcgcg
     2521 gcctccgact tcgtgcgcga gatccaccac tacctggggc ggcgggtcga cgcggtggtg
     2581 gtcaacacgg gcagcctgcc gccggagctg gtcgaacgct acgcgcgcca gggcgcggcg
     2641 ccggtcgcgg ccgacctcga cgtgctgcgc acgctcgtcc cgcgcgtgct gaccggctcc
     2701 gtcgccacca gcgagcgcct gatccgccac gacgccgagc gcgtgctgat gaccatctgg
     2761 ccggagctga acacgtagcg cgtggccccc gacgaccccg gctgccgcgc ttcgcacggc
     2821 aaccttcggc cgcgttccca ccccaatggg tggggaaacg actacccctc cgggtgtggc
     2881 gccgtgagta tgacatcctc atgtttagct caactcactc gcgcggacga gagatcgtgg
     2941 ggcacaacct gctctaatcg tcatgccatc gtaccgcagg ttgggaagcg aggtcgcctc
     3001 catggaccac tccgttggcc ggccatcctt gccccttcac gcggcattcg gtgacctggt
     3061 tcaggccgcc gtccgatccg cggccagctt cccagccggg ttccggcctg cggtcctggc
     3121 ggccagcttg agcgccctcc ggccgatcac gatggtgatc cagatcgtgc ttaccccaat
     3181 gacagtatgg cggggcacgg aagcttatct gagagacgtc cgtctgctcc accagctcaa
     3241 cctgtacccc ttgctgttcc tctcagcccg cgttccaagg ccagcggaac tcgagtccct
     3301 cgacgtgtcc tcgccgacac gttgatcgcg gaagggcagg gcgaggccat gaacatcacg
     3361 agcgtccgac tggatcgctt gccaagcgcc ctcg
//
LOCUS       DASUBI010000575         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_945835, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000575 DASUBI010000000
VERSION     DASUBI010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945835"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFU72_11135"
     CDS             <1..475
                     /locus_tag="VFU72_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424086.1"
                     /translation="TIATLLNWMADQQEVFVIATANDVRQLAPEQLRQGRFAQVIFVD
                     LPTPADRAEIFRVHLAKRGRDPEGFDLAALAAASEGFSGAEIEAAVKAGLLAAFLDGA
                     RSLTTADVLARVRTIRPTSEVKREEIAELRRWAQEHLALAAVGDTPAAGERLMEF"
     gene            575..970
                     /locus_tag="VFU72_11140"
     CDS             575..970
                     /locus_tag="VFU72_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424087.1"
                     /translation="MSKYLTFTDIVFTDRGLLLAALAGLGYPTVEEGELLALYGYRGD
                     QRPETAAIVVRRKYVGPLANDIGFARTPAGYVPILSEYDQRTVLGGRFLPALRLAYSE
                     AVVAAIQRRLRGTAQRTVEGQLVKIRVRY"
     gene            983..1189
                     /locus_tag="VFU72_11145"
     CDS             983..1189
                     /locus_tag="VFU72_11145"
                     /inference="COORDINATES: protein motif:HMM:NF022657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HEU5424088.1"
                     /translation="MPELDFTIDQTTGELQLHVQGIAGPACEEIAKLARTLLGTPVHE
                     EETAEYRLRPVVRPQARPQGEGQP"
     gene            1186..1875
                     /locus_tag="VFU72_11150"
     CDS             1186..1875
                     /locus_tag="VFU72_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HEU5424089.1"
                     /translation="MRGRTVTWILDRHTGQLTVEGLDAAALRTLAGDLLPPPQAVNCA
                     RPLAPPPLPADGAQPTADEPRLRVVRLYHGSVVEGPGRRSVVQTGGCVHHCSGCYAVE
                     THPLDAGVLLPVSTVVTALLDPGGAPRDGITVLGGEPLLQPDGLLVLLRAVKAAGQHV
                     TLYTGYSQTALARRDDANIPAILALTDLLIDGRYVAALADGAGEWRGSRNQQVIDLAA
                     RRRSGQTIHAG"
     gene            1971..3170
                     /gene="dnaN"
                     /locus_tag="VFU72_11155"
     CDS             1971..3170
                     /gene="dnaN"
                     /locus_tag="VFU72_11155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5424090.1"
                     /translation="MDLCVEQADLARALRLVERAVPSRPGTPALGGILITAAPGKLTL
                     AATDLALTLLAAVPAAVDQPGRALLPAQLLAGYVADLPTAVVQLTSEPEHNRVRARCG
                     RVAAAFAALDQAGFPTVPPPDLTLASALDARALRHALARVIPAAARDDSRPVLGAVLF
                     SFGADGATLAAADGFRLAYARLTVTAAPRTALVPVRAATEAVRLCGEADSVQLVFQPD
                     GQGLWLVAGATGLHTRLVAGRFPDIAALLPATWRTRVTVERLALRAALHQAALFGPAS
                     PGARPVVLSATPGWLRLGAWGAERGEVQSALPADLAGTPGMTALDTRLLVDLLAGAES
                     DRLTLTWDGPTGAVVIQEVAAAPTTTARHAGALWLVMPLQEPALLRRQAAAEAAATPE
                     TIAAPAA"
     gene            3255..3482
                     /locus_tag="VFU72_11160"
     CDS             3255..3482
                     /locus_tag="VFU72_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424091.1"
                     /translation="MTDHSPPRVFHTDLEGAYSEVAITFDDPPTFTFVRSPFNFTRPL
                     PRTELRELAAGRFGPDLDAQLRACTLPPTPP"
     gene            3710..4069
                     /locus_tag="VFU72_11165"
     CDS             3710..4069
                     /locus_tag="VFU72_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424092.1"
                     /translation="MLLTLRPDGPTGYRALVGVGTAGCDPHLATIEATGTLAAVLNAV
                     PEIVAAAETRWRATPRYPTVLRPAVLATVAPTKASSHPKAAVTKATGVAGAGIDSATP
                     DSSPAKPAATTQISLFG"
     gene            4136..>4340
                     /locus_tag="VFU72_11170"
     CDS             4136..>4340
                     /locus_tag="VFU72_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein C"
                     /protein_id="HEU5424093.1"
                     /translation="MARVFIVDGRQFPDPDPKLSVQEVRAQFAEFFPELVNADTREEV
                     RGDDTHITFTKRIGTKGAVRAASP"
BASE COUNT          658 a         1589 c         1463 g          630 t
ORIGIN      
        1 caccatcgcc accctcctga actggatggc cgaccagcag gaggttttcg tcatcgccac
       61 cgccaatgat gtgcgccagc tcgcccccga acaactgcgg cagggccgct tcgcccaggt
      121 catcttcgtg gacctgccaa cacccgccga ccgggccgag atcttccgtg tccacctggc
      181 caagcgcggg cgcgatcccg aaggcttcga tctggcggcg ctggcggcgg caagcgaagg
      241 cttctccggc gcggagattg aggcggccgt gaaagcgggc ctcctggccg cgtttctcga
      301 cggcgcccgc tcgctgacga cggccgacgt gctggcgcgc gtccgcacga tccgccccac
      361 ctcggaggtg aagcgcgagg agatcgccga gttgcggcgc tgggcacagg aacacctggc
      421 gctggcggcg gtgggggaca ccccggcggc gggcgagcgg ctgatggagt tctagaccaa
      481 cgttacccga gtgtggctgg acgcaccacc ttgccgctgt ggcaaagtgg atgcgtccag
      541 tcgccgtgcc acaagtgaaa ggagcacgcc agccatgtcc aagtatctga cgtttaccga
      601 catcgttttc acggaccggg gactgttgct ggcagcgctc gctgggctcg gctaccccac
      661 ggtcgaggaa ggcgagctgc tcgcgctcta cggctatcgc ggcgaccagc gcccggagac
      721 cgcggcgatc gtggtgcgcc gcaagtacgt cgggccgctc gccaacgata tcggcttcgc
      781 ccggacgcct gccggctatg tgccaatcct ctccgagtat gaccagcgca cggtcctcgg
      841 cggccgcttc ttgcccgccc tgcggctggc ctacagcgag gcggtggtcg cggccatcca
      901 gcgacgcctg cggggcacag cccagcgtac cgtggagggc cagctcgtga aaatccgcgt
      961 ccgctattga ggggagctga ccatgcccga actcgatttc accattgacc aaaccaccgg
     1021 cgaactccag ctccacgtcc aggggatcgc cggtccggcc tgcgaggaga tcgccaaact
     1081 cgcccggacc ctcctcggca cgccggttca tgaggaggag accgcggagt accgcctgcg
     1141 gccggttgtg cgaccacaag cacgacctca gggagagggg cagccatgag agggcggacg
     1201 gtgacctgga tcctggatcg acacaccggc cagcttaccg tcgaagggct ggacgcagcg
     1261 gcgctacgga cgctggccgg cgacctgctg cccccaccgc aggcggtgaa ctgcgcgcgg
     1321 cccctcgctc cgccaccact cccggcggac ggggcgcaac caaccgctga tgagccgcgc
     1381 ctgcgggtcg tacggctcta ccacggctcg gtagtcgagg gaccagggcg acggagtgtc
     1441 gtccagaccg gtggctgtgt tcaccactgc tctgggtgct atgcggtcga gacccatccg
     1501 ctcgatgccg gcgtgctgct gcccgtctcc accgtcgtca cagcgctgct cgacccgggc
     1561 ggcgcgccgc gcgacggcat caccgtactc ggtggcgagc cgctgctcca accggacggg
     1621 ctactcgtgc tcctacgtgc ggtcaaggcg gcggggcagc acgtcacgct ctacaccggt
     1681 tactcgcaga ccgcgctcgc gcgtcgggat gacgccaaca ttccagcgat cctggcgctg
     1741 acggacctgc tcattgacgg gcgctatgtc gcggccctgg cggacggcgc aggcgaatgg
     1801 cgcggctcgc gcaaccagca ggtcatcgat ctcgccgcgc gccggcgatc cgggcagacg
     1861 atccacgccg gataagcatc cagccgaaca cgatgcatag tgacagctat cccagcctgg
     1921 ggcaccctgc caccaggcag cgtggtgaca gcagagacag gagcacgatc atggatctct
     1981 gtgtcgagca agccgacctc gcccgcgccc tgcggctggt cgagcgcgcc gtgccgagcc
     2041 gtcccggcac tcctgccctc gggggcatcc tgataacggc ggctccaggc aaactgacgc
     2101 tggcggcgac cgacctggcg ctgacactac tggcggcggt gcccgccgcc gtggaccagc
     2161 ccgggcgggc gctgctgccc gcccaactgc tcgctggcta cgtcgctgac ctcccgaccg
     2221 ccgtcgtgca gctgaccagc gaaccggagc acaaccgggt tcgggcgcgc tgtgggcggg
     2281 tagcagcggc cttcgcggcg ctggaccagg caggctttcc caccgtaccg ccgccggacc
     2341 tgacgctggc cagcgcgctt gacgcgcggg cactgcggca cgcgctcgcg cgggtcatcc
     2401 cggcggcggc gcgcgacgac agccggcccg tcctcggcgc ggtcctgttc tcgttcggcg
     2461 cggatggggc cacgctggcc gcggcggatg gtttccggct ggcctacgcg cggctcactg
     2521 tcacggccgc accacgaacg gcgctggtgc ctgtccgcgc cgccaccgaa gcggtgcgac
     2581 tctgtggcga ggccgatagc gtacaactgg tgttccagcc tgacggtcag ggactgtggc
     2641 tcgtggctgg cgcgaccgga ctccacacac gcctcgtcgc gggccgcttt cccgatatcg
     2701 ctgccctgct gccggcaacg tggcgtaccc gagtgacggt ggagcgcttg gcgctacggg
     2761 ccgcgctcca ccaggcggcg ctcttcggtc ccgctagccc tggcgcgcgg cccgtcgtcc
     2821 tctccgctac ccctgggtgg ctgcggctcg gcgcctgggg cgctgagcgt ggcgaagtcc
     2881 agagcgcgct cccggccgac ctggcgggca cgccaggcat gaccgcgctc gacacgcgcc
     2941 tgctggtgga tctgttggcc ggcgccgaga gtgaccggct gactctcacc tgggatggac
     3001 cgacgggggc ggtcgtgatc caggaggtgg ccgccgcacc gaccaccact gctcggcacg
     3061 ctggcgccct gtggctggtg atgccgctcc aggagccggc tttgctgcgc cgccaggccg
     3121 ccgctgaagc cgccgccacc ccggagacca ttgccgcacc agcggcctga acccggcagc
     3181 gggcacgctc gcgcgccgcc ggacggagct ggtagcgagg gcacgacact cagcactgta
     3241 tcaaggagca accaatgaca gatcactcgc ctccacgcgt gttccatacc gacctcgaag
     3301 gcgcctacag cgaggtcgcg atcaccttcg atgacccacc gactttcacg ttcgtccgca
     3361 gccccttcaa cttcacgcgc ccgctgccgc gcactgagtt gcgtgaactg gcggcgggcc
     3421 gctttggccc ggacctggac gcccagctgc gcgcctgcac gctgccgccc acccccccct
     3481 gagcgacgac gatcgggcgc gcatcgtccg gctggccggc gacgcctggg ccgctgcgga
     3541 gaccaccgcg cagaccggcg gcgctggggt gctgggcggc gagcagtcct ctagcatgcc
     3601 caacgcgctg ccacccgcca cacacccgcc cagtcccata gcgcctgcta gtctggcgag
     3661 cacgcccccc agcgcggcgg tcgccccgac gccgcgccgc gagttgaagg tgctgctgac
     3721 gctccggccc gatggcccca ctggctaccg cgccctcgtc ggcgtgggca ccgctggctg
     3781 cgatccacac ctcgcgacca tcgaggcaac tggcacgctc gccgcagtcc tgaacgcggt
     3841 gccggaaatc gtcgccgccg ccgagacgcg ctggcgcgcg acaccgcgct acccgactgt
     3901 acttaggccg gcggtattgg cgactgtcgc gccgacgaag gcgtcatctc accccaaagc
     3961 cgccgtgacc aaggcgaccg gggtagccgg cgcggggatc gactcggcca ccccggacag
     4021 ttcgcccgcg aagcctgcgg caacgacgca gatcagcctg ttcggctaat agtacacagc
     4081 ggcgtctgtc atcgcggcgg acgctgctgc cctgagcaca gttggaggta gcatcatggc
     4141 gcgtgtcttc atcgtcgatg gccggcagtt ccccgatccc gacccgaagt tgagcgtcca
     4201 ggaggtgcgc gcacagttcg cggagttctt ccccgagctc gtcaacgccg acacccgcga
     4261 agaggttagg ggcgacgata cccacatcac cttcacgaaa cgcatcggga cgaagggggc
     4321 ggttcgcgcc gcttcccccg
//
LOCUS       DASUBI010000576         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_241722, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000576 DASUBI010000000
VERSION     DASUBI010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241722"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1887)
                     /locus_tag="VFU72_11175"
     CDS             complement(109..1887)
                     /locus_tag="VFU72_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5424094.1"
                     /translation="MTLSTTDLIRVGRLDELTGPRVVSGGRHGIAVFLSDGVPYAVDN
                     RCPHMGFPLQRGSVCDGILTCHWHHARFDLASGGTFDPFADDVRTYETVVEDGVVYVN
                     PRPRPLDLERHWKRRLREGLEQNLSLVLIKAVLGLREAEVPERDVVAIGGHFSATYRQ
                     AGWGPGLTILTAMANVLPALERTDAALALYHGLVHVSRDALNEPPRFQQEPLPERETP
                     LPRLKTWFRQFIEVRDGVGAERALLSAIDSGAGPAELMDLLFAAATDHVFLDGGHTLD
                     FINKAGELLDGVGWDQARDILPSVVPGLAMARRSEELNNWRHPVDLVRLIEPALARLP
                     ELAGGTTAGDWDDFDGLAATLLGDDPEAIVDGLMAALESGATLTQLSQVLSYAAALRV
                     ARFHTSNEFSDWITVLHTFTAANALHQGLKRSPSPELARGLFHGAARLYLDRFLNMPA
                     ARLPERRNAETEPVDTSELLDKLADLLDREQQVNEAALVVHRYLSLGHERAPLLAALG
                     MTLVREDAEFHSYQMLEAGLALERELRDARPAAANRVLVAIARYLAAHAPTSRAMLQT
                     ARTAMRLQRGEAVYEDAEELAEVADA"
     gene            2116..2928
                     /locus_tag="VFU72_11180"
     CDS             2116..2928
                     /locus_tag="VFU72_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424095.1"
                     /translation="MTANRVPRLVQLLAAAFLVGGAAGALGTFSLTEGIWVWPMSALA
                     MRFLAAAASAYAVGGLLALRRRTWPANVLLMSSAVVYAVPLLLALALRTDPIDWSRAQ
                     SWGFVAIITVALAISVYYLTSGDRWRSGSAEPLSGPLRVGAMLLGTVALVVGVIVFSA
                     PRQAGFIWPWAELGSWKVLDSRLIASMLITIGAEALLVAWWNDRAKAAVFLPMLWAYG
                     VMAILGLALHAAKTPALRFDDLLYSTIFLAIVAGSVVIHLTQDRLPRLATSV"
     gene            complement(3009..3743)
                     /locus_tag="VFU72_11185"
     CDS             complement(3009..3743)
                     /locus_tag="VFU72_11185"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5424096.1"
                     /translation="MALDKPIQANPPAGEYDQHLDRLLALSDGVFAIALTLLSIDLHL
                     PEAAAELSGHALFTSLLDTWPKLLSYVTSFTVIAIYWIAYHRVFHLVRRLDGPLLGLT
                     LVLLAFIAFMPFPTSVLGTHVGDAVAQEFYYGSLSALSIVSAVMWQYASAGRRLVDRN
                     LSLETIRYYRRLALSAPAFFLLMMVVIAIGLGNVINPLIVGYVLGLSYAVLLLVSEER
                     WSADRSLSAAADMMPDEPDAVPYDVS"
     gene            complement(3858..>4698)
                     /locus_tag="VFU72_11190"
     CDS             complement(3858..>4698)
                     /locus_tag="VFU72_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424097.1"
                     /translation="PLTPAMRWVFWIGCVFVLIAGFQLFVLSEHTLRFFAWTVNPPLT
                     AAVMGAFYWTALVVAFLSLRQRDWTNARVGVPGVLLFLILSLSATLVHLDKFHLHDER
                     LVTRLAAWFWLIIYILDPILVVLALWLQLRVPGRSSARTATLPAAYRAVLLTLGGALL
                     GLGLALFVAPTTVASSWAWALTPLTARAVGAWSAGTGVMLGQAAWENDRHRIRPAAWG
                     ALTLGSLQLLAIARYSGQFRWEHPAAWLWLAGVIVMLGVGLYGPFIGTRASGTLAQSS
                     GSK"
BASE COUNT          768 a         1608 c         1633 g          689 t
ORIGIN      
        1 taacattatg acgagtaggc gcacgtcagt cccgtcactt cccgcgattc ctccagggcc
       61 acgaaggaca agttggcact cgcccacagg ggtgacggcc cgtccgtctc aggcgtcggc
      121 tacctcggcc agctcttccg cgtcctcgta gacggcctca ccacgctgca ggcgcatggc
      181 cgtgcgcgcg gtctggagca tggcgcgcga ggtcggcgcg tgcgcggcca gatagcgcgc
      241 gatcgccacc agcacgcggt tggcggcagc gggccgcgcg tcgcgtagct cgcgttccag
      301 cgccagcccc gcctccagca tctggtagct gtggaactcg gcgtcctcac gcaccagcgt
      361 catgccgagc gccgcgagca acggcgcgcg ctcgtgcccc aggctgaggt agcggtgcac
      421 caccagcgcc gcctcgttga cctgctgctc gcggtcgagc aagtcggcca gcttgtccaa
      481 caattcgctg gtgtcaactg gctccgtctc cgcgttgcgg cgctctggca ggcgcgccgc
      541 cggcatgttc aggaagcggt cgaggtagag ccgggccgcg ccgtggaaga gcccgcgcgc
      601 cagctcgggc gagggcgagc gcttgaggcc ctggtgcagg gcattggccg cggtgaaggt
      661 gtgcagcact gtgatccagt cggagaactc attactggtg tggaagcgcg ccacgcgcag
      721 cgcggcggcg tagctcagaa cctggctgag ctgggtcagg gtcgcgccgc tctccagcgc
      781 cgccatcagc ccgtcgacga tcgcctccgg gtcgtcaccc aggagcgtgg cggccagccc
      841 gtcgaagtcg tcccagtcac ccgcggtggt accccccgcc agctccggca ggcgcgccag
      901 ggccggctcg atcagccgca ccagatcgac cgggtgccgc cagttgttca gctcctcgct
      961 gcgccgggcc atcgccaggc cgggcaccac gctgggcagg atgtcgcgcg cctgatccca
     1021 gccgacaccg tcgagcagtt cccccgcctt gttgatgaag tcgagggtgt ggccgccgtc
     1081 gaggaagacg tggtcggtcg cggcggcgaa gagcaggtcc atcagctccg ccggtccagc
     1141 gccgctgtcg atcgcgctga gcagcgcccg ctcggcgccc acgccgtcac gtacctcgat
     1201 gaactgacgg aaccaggtct tgagccgggg cagcggcgtc tcgcgctccg gcagcggctc
     1261 ctgctggaaa cgcggcggct cgttgagcgc gtcgcggctg acgtggacca gtccgtggta
     1321 gagcgccagc gcggcgtcgg tgcgctccag cgccggcagg acattggcca tcgcggtcag
     1381 gatggtcagc ccgggtcccc atcccgcctg gcggtaagtg gcgctgaagt gcccgccgat
     1441 cgcgaccacg tcgcgctcgg gtacctccgc ctcgcgcagc ccaagcacgg ccttgatcag
     1501 caccagcgag agattctgct ccagcccttc gcgcaggcgg cgcttccagt ggcgctccag
     1561 gtcgagcggg cgcgggcgcg ggttgacgta gacgacgccg tcctcgacca ccgtctcgta
     1621 ggtgcgcacg tcgtcggcga agggatcgaa ggtgccgccg ctggccagat cgaagcgggc
     1681 gtggtgccag tggcaggtca gaatgccgtc gcagacgctg ccgcgctgca gcgggaagcc
     1741 catgtgcggg cagcggttgt caaccgcgta ggggaccccg tcgctgagaa acacggcgat
     1801 gccgtgccgg ccgcctgaga ccacacgcgg cccggtcagc tcgtccaggc ggccgaccct
     1861 gatcaggtca gtggtgctga gtgtcatgag ctgctcctct cgccatcggc gcgtccacgc
     1921 gcgcgacaac cctggcctgc atggctccta tagtactcgg agctgtgtct tcgcgcagcc
     1981 ggccaccagt cgttgacgag cggcgattca ccggtatgat gggcgaagcg cacgccaatc
     2041 tccaatcatc ggttcaacat acgagggaat ctcccgaccg tcgcgcgacg aagacacgca
     2101 aaagggggtg tgggagtgac ggcgaaccgc gtgccccgtt tggtgcaact cctggccgcg
     2161 gccttcctgg tgggtggcgc ggccggcgca ctcggcacgt tctcgctgac cgagggcatc
     2221 tgggtctggc cgatgtccgc gctggcgatg cgcttcctcg ccgcggcagc cagcgcctat
     2281 gccgtcggcg gcctgctggc gctgcggcga cgcacgtggc ccgcgaacgt gctcttgatg
     2341 tcgagcgcgg tcgtctatgc cgtgccgctg ctgctggcgc tcgcgctgcg cacggacccc
     2401 atcgactgga gccgggcgca gtcctggggc ttcgtcgcga tcatcaccgt cgcgctggcg
     2461 atcagcgtct actacctgac ctcgggagat cgctggagaa gcggctcggc ggagccgctc
     2521 tccggcccgc tgcgcgtcgg agcgatgctg ctcggcacgg tagcgctcgt ggtgggtgtg
     2581 atcgtcttca gcgcgccgcg ccaggcgggc ttcatctggc cctgggccga actgggaagc
     2641 tggaaggtgc tcgacagccg cctgatcgcg tcgatgctga tcaccatcgg cgccgaggcg
     2701 ctgctggtcg cctggtggaa cgaccgcgcc aaagcggcgg tcttcctgcc gatgctctgg
     2761 gcgtacggcg tgatggccat tctcggcctg gcgttgcacg ccgccaagac gcccgcgctc
     2821 cgcttcgatg atctgctcta cagcaccatc ttcctggcga tcgtcgccgg cagcgtcgtc
     2881 atccacctga cacaagaccg cctgccgcgg ctcgcgacgt cagtctaatc gaccgagcgg
     2941 tcaggccggc gcgcgcccgc gacttctccc attgggacgc ccggctcgcc gtccggtctc
     3001 ggcccctatt acgaaacgtc gtacggcacc gcgtccggct catcgggcat catgtcggcg
     3061 gcagccgaca gcgaccggtc ggcggaccag cgctcctcgc tgaccagcag cagcacggcg
     3121 tagctgaggc cgagcacata gcccacgatc agcgggttga tcacgttgcc cagaccgatg
     3181 gcgatgacca ccatcatcag caggaagaag gccggggccg acagcgccag gcggcgatag
     3241 tagcgaatgg tctccagact caggttgcgg tcgaccaggc gccggccggc gctggcgtac
     3301 tgccacatga ccgcgctgac gatcgagagc gcggacaggc taccgtagta gaactcctgc
     3361 gccaccgcat cgccgacgtg cgtgccgagg accgaggtcg ggaacggcat gaacgcgatg
     3421 aaggcgagca gcacgagcgt gagcccgagc agtggcccgt cgaggcgccg caccagatga
     3481 aagacgcggt gataggcgat ccagtagatc gcgatcaccg tgaagctggt gacgtagctg
     3541 agcagcttgg gccaggtgtc gagcaggctg gtaaagaggg cgtgcccgct cagctcggcc
     3601 gccgcctccg gcagatgcag gtcgatcgac agcagcgtca gcgcgatggc gaagacgccg
     3661 tccgacagcg ccagcagccg gtcgagatgc tggtcatact ctccggcggg cgggttcgcc
     3721 tggatcggtt tgtcaagcgc catgctgttc cctctctgga tcggtcgccg tcgccaccgt
     3781 cgacgccctc tgaggatggg cacagccccg gactggcacg aagcgccaac gagcggtcgt
     3841 ctgccggtgg gggtggccta cttggaaccg ctgctctggg cgagcgttcc ggacgcgcgc
     3901 gtcccgatga atggcccgta caatccgacg cccagcatga cgatcacgcc ggccagccac
     3961 agccaggccg ccggatgttc ccaacggaac tggccggagt agcgggcaat ggccagcaat
     4021 tgtaaagaac cgagcgtcag cgcaccccag gccgccgggc ggatgcggtg ccgatcgttc
     4081 tcccaggccg cctgccccag catcacgccg gtgccagcag accaggcgcc gacggcccgc
     4141 gcggtcaacg gggtgagtgc ccaggcccag gacgacgcga ccgtcgtcgg cgcgacgaac
     4201 agagcgagcc cgagccccag cagcgcgccg ccgagagtca gcaagacggc gcgataggca
     4261 gccggcaacg tggctgtccg agccgagcta cgccccggca cgcgcaactg cagccacaac
     4321 gcgaggacga ccagaatcgg gtcgaggatg tagatgataa gccaaaacca ggcggcgagc
     4381 ctggtgacga ggcgctcgtc gtgcaagtgg aacttgtcca gatgcaccag cgtcgcgctc
     4441 aggctcagaa tgagaaagag gaggacgccc ggcacgccca cgcgcgcgtt ggtccagtcg
     4501 cgctggcgca agctcaggaa ggccaccacc agcgcggtcc agtagaaggc gcccatgacc
     4561 gcggcggtca gcggcgggtt gaccgtccag gcgaagaagc gcagcgtgtg ctccgagaga
     4621 acgaagagct ggaagccggc gatcaggacg aagacgcagc cgatccaaaa gacccagcgc
     4681 atcgcgggcg tcagcggt
//
LOCUS       DASUBI010000577         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1242445, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000577 DASUBI010000000
VERSION     DASUBI010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242445"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFU72_11195"
     CDS             <1..219
                     /locus_tag="VFU72_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424098.1"
                     /translation="LALWDRGQVRQGFWADLVIFDPKTVGDVATFEQPHQLSVGVRDV
                     WVNGTRVVQEGEHTGATPGHFVKGPGAR"
     gene            749..>2622
                     /locus_tag="VFU72_11200"
     CDS             749..>2622
                     /locus_tag="VFU72_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424099.1"
                     /translation="MGEDRLRAGRLLALRAELSRRALLKRAAAGGISMAAVISLLEAC
                     GGSSSTPAATTAATGTSSSGAGGSTATAAATSAPAAAGSPVASAAATSSGAATPSAQG
                     TQSSTVSKTFNIEPAKSKGGQLVWGSSSDAQTANSILSKDTTSGLIISMMFNGIMRTD
                     PTNAQPIGDLAKSFEVTPDGMKYSFVLLDGVKFHDGQALTANDVKFTYDMAMNGATGS
                     PRTGELTTRIASLDVADDSHFSVSMKIPVSSFLVSNMQYGILPQHILKDVAPADLAKS
                     DFSTGKKGVTIGTGPFMFEEWVKDDHVTCTKYAGYFKGAPNLDRWIMKVVPNATTLTQ
                     QLKTGEVDTGTIQPPDYQDMQKQNNINVVAYDTFSFTFYSYQLDTSKTTLFQDKAVRQ
                     ALLYGLDRKAMIEAIYFGLGSVAVGTMPVLSWAYNPDAITLKYPYDTKKAGDLLDGAG
                     WKMGSDGIRAKDGKKLTINFWTSTASQQFVEDSQVMQEQWKKIGVDMKPNTEEWNAFI
                     DRITKTFDFDMFLVGFSWGVDPDQTAMWATDSYPSGFNMGKYSNPKVDDLLKQGLATI
                     DQAKRKELYTQMQNILMDDLPNAVLFFTKAIYGVNKRVHNFFPNAVSIPFNSYQWWVD
                     A"
BASE COUNT          493 a          878 c          818 g          433 t
ORIGIN      
        1 ctcgcgctct gggaccgggg ccaggtgcgt cagggcttct gggccgactt ggtcattttc
       61 gatcccaaga cggtgggcga cgtggccacc ttcgagcagc cgcatcagct ctcggtcggc
      121 gtgcgcgacg tctgggtcaa cggcacgcgg gtcgtgcagg agggcgagca caccggtgcc
      181 acccccggcc acttcgtcaa gggccccggc gccaggtagt cttcgcgtca ccacgggtaa
      241 cgacggccgg tgcggcagtc gcaccggccg ttcaatttcg ggcgctcggc gcgcgaaacc
      301 gaccaggagc cggtaggaaa gctcaaattt ctgccaagaa tgaaccgatt gatgcgccgg
      361 gacgtacata gctacaaggt cgctgcccgc gcatggggag ttcggcgcgg gtgggagccg
      421 ccggcaggtc gggttctgtt ggcgtgtggt gatgggcaac aacgtctcgt ggcgtggggg
      481 cggacgtgtc gcaccggctg gtcgggagac caacccgagt tacgggatat tgacaagtca
      541 cgccgcgatt cataggctgt aaacgttgtt gcacaccggg cgcctgtttc agtgtggttc
      601 atcatgctgc gtgtgagcgg cgagtgcccg cgctgatcct gccgggttgt tcacgtcgcc
      661 ttggagcagc gcccatgccg gccggggccg ccctgggact gctccgttcc ggtgtgttgt
      721 ggctatccct tatgtgagga ggggttccgt gggtgaagat agactgcgcg cggggcgctt
      781 gctggcgctg cgcgcggagc ttagtcgtcg tgcgttgctg aagcgcgcgg cggccggcgg
      841 catcagcatg gccgcggtca tcagcttgct cgaggcctgc ggcggcagca gcagcacccc
      901 cgccgccacc actgccgcca ccggcaccag cagcagcggc gccggcggca gcaccgccac
      961 cgcggccgcc actagcgcgc ccgccgcggc cggctctccg gttgcttcgg ccgccgcgac
     1021 cagctccggc gccgccaccc cgagcgcgca gggtacgcag tcctccaccg tctccaagac
     1081 cttcaacatc gagccggcca agagcaaagg cgggcagctc gtctggggct cgtcctccga
     1141 tgcccagacc gccaactcga tcctctccaa ggacaccacc tccggcttga tcatctcgat
     1201 gatgttcaac gggatcatgc gcaccgaccc caccaacgcc cagccgatcg gcgatctggc
     1261 caagtcgttc gaggtcacgc ccgacggcat gaagtactcc ttcgtgctcc tcgacggcgt
     1321 caagttccac gacggccagg ccctgaccgc caacgatgtc aagttcacct acgacatggc
     1381 gatgaacggc gccaccggct cgccacgcac cggcgagctg accacgcgca tcgcctcgct
     1441 cgacgtggcc gacgacagcc acttctcggt cagcatgaag atcccggtct cgtccttcct
     1501 ggtcagcaac atgcagtacg gcatcctgcc acagcacatc ctcaaggatg tggcgccggc
     1561 cgacctggcc aagagcgact tctcgaccgg caagaagggc gtgacgatcg gcaccggccc
     1621 cttcatgttc gaggaatggg tcaaggacga ccacgtcacc tgcacgaagt acgccggcta
     1681 tttcaaaggc gcgccgaacc tcgaccgctg gatcatgaag gtcgtgccca acgccaccac
     1741 gttgacccag cagctcaaga ccggcgaggt cgacacgggc acgatccagc cgccggacta
     1801 tcaggacatg cagaagcaga acaacatcaa cgtcgtggcc tacgatacct tcagcttcac
     1861 cttctactcc taccagctcg acacctcgaa gacgacgctc ttccaggaca aggccgtgcg
     1921 ccaggcgctc ctctacgggc tggatcgcaa ggcgatgatc gaggcgatct acttcgggct
     1981 gggctcggtg gcggtcggca ctatgccggt cctctcctgg gcctataacc cggacgccat
     2041 cacgctcaag tacccctacg acaccaagaa ggcgggcgac ctcctcgacg gcgccggctg
     2101 gaagatgggc tcggacggca tccgcgccaa ggacggcaag aagcttacga tcaacttctg
     2161 gaccagcacc gccagccagc agttcgtcga ggactcgcag gtgatgcagg agcagtggaa
     2221 gaagatcggt gtggacatga agcccaacac cgaggagtgg aacgccttta tcgaccgcat
     2281 caccaagacc ttcgacttcg acatgttcct ggtcggcttc tcctggggcg tcgacccgga
     2341 ccagaccgcc atgtgggcca ccgactccta ccccagcggc ttcaacatgg gcaagtacag
     2401 caatccgaag gtggacgacc tgctcaagca ggggctggcg acgatcgatc aggccaagcg
     2461 caaggagctg tacacgcaga tgcagaacat cctgatggat gatctgccca acgcggtgct
     2521 cttctttacc aaggcgatct acggcgtcaa caagcgggtg cacaacttct tcccgaacgc
     2581 cgtcagcatc cccttcaact cgtatcagtg gtgggtggac gc
//
LOCUS       DASUBI010000578         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_268401, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000578 DASUBI010000000
VERSION     DASUBI010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268401"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /gene="menE"
                     /locus_tag="VFU72_11205"
     CDS             <1..1349
                     /gene="menE"
                     /locus_tag="VFU72_11205"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEU5424100.1"
                     /translation="QLASVGAGAGDRIATLLTNSHHVPPLVHAMGRLGTVMLPLNIRL
                     SAPELAWQLQDAGAALLLCDARTAPAAAEAARALTGLTLLDVAALAALPEAPVPLRSG
                     LDLAATHSIVYTSGTTGRPKGALLSYGNHWWSATGSAMNLGSQPDDRWLAVLPLFHVG
                     GMAILLRSVISGFTAEVHERFDPEAVNQAIDEGVTIVSVVSAMLRRMLAARGDRRYPP
                     TLRCVLLGGGPAPQPLLEVCAARGIPVTQTYGLTETDSQVATLAPEDALRRLGSAGKP
                     LYPNALRIAEDGRELTPGQAGEILVRGPVVSAGYAGRPEATAEALRDGWLHTGDLGYL
                     DADGYLFVLDRRGDLIVSGGENVYPAEVEAALLAHPAVVEAGVIGLPDPDWGQVPTAC
                     VRLSDGAALSPDDLIVFCAGRLARYKVPRQIRFVAALPRNATGKLLRRQLREEWPG"
     gene            1568..1999
                     /locus_tag="VFU72_11210"
     CDS             1568..1999
                     /locus_tag="VFU72_11210"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5424101.1"
                     /translation="MPAVVRSIEIEAPPSVVWRWLATPEGLRRWISPNLEIDLRVGGA
                     YRLLNPGEETWISGTVLELVPEGELILSWLEEGGDWVYPARLVFSLTPTPVGTRVTLA
                     HDGFAGIGKPGWPDTLRAYERGADRHQVLERLADLVLAGVV"
     gene            1992..2327
                     /locus_tag="VFU72_11215"
     CDS             1992..2327
                     /locus_tag="VFU72_11215"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5424102.1"
                     /translation="MSSTTAELFRVLADPTRRRILDLLAERGPLTVNQLAAEFPDLVV
                     SGISKHLMVLRGAGLVSATPQGRQRLYRIDAQGLADALAPWLAKYEPYWAGALQRLRD
                     LAEGEEPPA"
     gene            complement(2543..>2924)
                     /locus_tag="VFU72_11220"
     CDS             complement(2543..>2924)
                     /locus_tag="VFU72_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5424103.1"
                     /translation="CGAPVFDLEAMVGTSDISHRTSDQQWGGTPATTPEAITWYETHS
                     PSSYAQRATTPTLIVHGEADERCPIGQSEQMFITLKEAGCEVEFARYPGGSHGFPRLG
                     PPEHRVDFLQRTLDWFKAHLGEPK"
BASE COUNT          396 a          980 c         1082 g          466 t
ORIGIN      
        1 ggcaactggc gagcgtcggt gctggagcgg gcgaccggat cgcgacgctg ctgaccaaca
       61 gccaccacgt gccgccgctg gtgcacgcca tgggccggct gggcacggtg atgctgccgc
      121 tcaatatccg gctcagcgcg ccggagctag cctggcagct ccaggacgcc ggcgccgcgc
      181 tgctgttgtg cgacgcgcgc acggccccgg cggcggcgga agccgcgcga gcgctaactg
      241 gcctaacgct gctcgacgtc gccgcgctgg ccgcgctgcc cgaggcgccc gtgccgctgc
      301 gctcggggct ggatctcgcg gcgacgcaca gcatcgttta cacctccggc acgaccgggc
      361 gtcccaaggg cgcgctgctc agctacggaa accactggtg gagcgccacc ggctcggcca
      421 tgaacctcgg cagccagccc gatgaccgct ggctggcggt gctgccgctc ttccacgtcg
      481 gcggcatggc gatcctgctg cggagcgtga tctcgggctt caccgccgag gtccacgagc
      541 gcttcgatcc ggaagcggtc aaccaggcga tcgacgaagg tgtgaccatc gtctcggtag
      601 tgtcggccat gctgcggcgc atgttggcgg cgcgcggcga tcggcgctac ccgccgacgc
      661 tacgctgcgt gctgctcggc ggcggcccgg cgccccagcc gttgctggag gtgtgcgccg
      721 cgcgcggcat tccggtgacg cagacctacg gcctgaccga gacggattcg caggtggcga
      781 cgctggcgcc ggaggacgca ctgcgccggc tcggctcagc cggcaaaccg ctctacccca
      841 acgcgctgcg catcgccgag gatggacggg agctgacgcc gggccaggcg ggcgagatcc
      901 tggtgcgcgg gccggtagtg agcgcgggtt acgccgggcg ccccgaggcg acggccgagg
      961 cgcttcgcga cggctggctg cacaccggcg acctcggcta cctcgacgcc gacggctacc
     1021 tctttgtcct cgaccggcgc ggcgacctga tcgtctccgg cggcgagaac gtctacccgg
     1081 ccgaggtcga ggccgcgctg ctggcccacc cggcggtcgt cgaggcgggc gtgatcggcc
     1141 tgccggaccc cgactggggc caggtgccga cggcctgcgt gcgtctgagc gacggtgccg
     1201 cgctcagccc cgatgacctg atcgtgttct gcgccggtcg gctggcgcgc tacaaggtac
     1261 cgcgacagat tcgcttcgtg gctgcgctgc cccgtaatgc gacgggcaag ctgctgcggc
     1321 ggcagttgcg agaggagtgg ccgggctgac gcgtggcgct ggcgcattcg aggacgaatc
     1381 ctgcgactgc tctcaggatg accgggggga ggggatgggg cctttcggac gaggcgagcc
     1441 gtcgtcagac cgtatcaacc ctgtgctggc tgatggggcg ccgaggccct tgacatgaac
     1501 ctcatcttac tgtagagtaa tgcgttagag acaccttact gttgggtaat acgtggaggt
     1561 gatggccgtg cctgctgtcg tccggtcgat cgagatcgag gccccgccga gcgtcgtctg
     1621 gcgctggctg gccacgccgg aggggctgcg ccgctggatc agccccaacc tggagatcga
     1681 tctgcgtgtc gggggcgcct atcgcttgct caatcccggg gaggagacct ggatcagtgg
     1741 caccgtgctg gagctggtgc ccgagggcga gctgatcctc tcctggctgg aggagggcgg
     1801 agactgggtc taccccgcgc ggctggtctt ctcgctgacc ccgacgcccg tcggcacgcg
     1861 cgtcaccctg gcgcacgacg gcttcgccgg tatcggcaag cctggctggc ccgacacgct
     1921 gcgggcctac gagcgcggcg ccgaccggca ccaggtgttg gagcggctcg ccgatctggt
     1981 gctggcgggc gttgtctagc acgaccgccg agctcttccg ggtgctggcc gatccgacgc
     2041 gccggcgcat cctcgacctg ctggccgagc gcggcccgct gacggtcaac cagttggcgg
     2101 cggagttccc cgacctggtg gtgtccggca tctccaagca cctgatggtc ttgcgtgggg
     2161 cggggttggt cagcgcgacg ccgcagggcc ggcagcggct ctaccgcatc gacgcacagg
     2221 ggctggcgga cgcgctggcc ccctggctgg ccaaatacga gccgtactgg gccggtgcgc
     2281 tgcagcggct acgcgatctt gccgaagggg aagagccgcc cgcgtgagct ggcggctggt
     2341 agccgggctg acgacgacgc cgggtgccag gcggggacgg atccttcgac aggctcagga
     2401 tgacatgaac gagggagggg cggatgacta cgtcacatcg tagtcgtccg cccctccctg
     2461 tctctgtcat cctgagcgta gtcgaaggat ccgtcttgca ctacgtcctt cgcttgtcgg
     2521 aggctaccgc ctcttcccgc gcctacttcg gctctccgag gtgcgccttg aaccaatcca
     2581 gcgtgcgctg caggaagtcg acgcggtgct cgggcgggcc gaggcgcggg aagccgtggg
     2641 agccgccggg gtagcgggcg aactcgacct cgcagccggc ctctttgagc gtgatgaaca
     2701 tctgctcgct ctggccgatc gggcagcgct cgtccgcctc gccgtgcacg atcaacgtcg
     2761 gcgtggtggc gcgctgggcg tagctggagg gcgagtgcgt ctcgtaccag gtgatcgcct
     2821 cgggcgtggt agcgggcgtg ccgccccact gctggtcgct ggtgcgatgg ctgatatcgc
     2881 tggtgccgac catagcctcg agatcgaaga cgggcgcgcc gcag
//
LOCUS       DASUBI010000579         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_749595, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000579 DASUBI010000000
VERSION     DASUBI010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749595"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFU72_11225"
     CDS             complement(<1..320)
                     /locus_tag="VFU72_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424104.1"
                     /translation="MSVEGPPGYHLTRQERIPLERVSLLSLALAPLWLALFVALARVL
                     GTSRPRIRLGLPSIAGGLALLTLVVPLAHEAIHGLVARALGARPRFGLGAGFAYTTFA
                     EPVSR"
     gene            complement(317..1312)
                     /locus_tag="VFU72_11230"
     CDS             complement(317..1312)
                     /locus_tag="VFU72_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5424105.1"
                     /translation="MRNPLEAIVHKVVRQLSPEAEAITVLDDQTLDGRLLWHVRALDV
                     QWRSGEQVCRRRLVVKREVQVEAGESAVLRALAGSGLPIPTVLHIEPDDLANYLVMDF
                     LPGSVLFPVMQSSEARWEIAAAATAYAHTLARIHALDWRALVPWMSDPTGPETEIVLG
                     QIQGLWSHWDERIREAPDSLKPLFDSAQNWLSRHMIETASLCLCHGDYTPANLLVEDG
                     EISGVVDWDRAQVTDPAWDLALLPFSVYRAGMPAADADLLVQTALGAYGESSPHSWQN
                     LPFFIIARLLDGALDVLVTGDGEPGLLAPTDPTLDDYVHALERAMPRGGQLPWRQ"
     gene            1449..1619
                     /locus_tag="VFU72_11235"
                     /pseudo
     CDS             1449..1619
                     /locus_tag="VFU72_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            1623..1775
                     /locus_tag="VFU72_11240"
     CDS             1623..1775
                     /locus_tag="VFU72_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEU5424106.1"
                     /translation="MTAPPVPSQSVPTTTTTITSNSATPTCATCGAPIPNGAHFCANC
                     GSKVAA"
     gene            1801..>2391
                     /locus_tag="VFU72_11245"
     CDS             1801..>2391
                     /locus_tag="VFU72_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5424107.1"
                     /translation="MNFFGMGPLEILAITLVAFIIFGPEKLTEIMGGLGRAVREFRSM
                     SSDLTGEFERTINEVKETAGDLQSTTRDALTVEGLNPFDQPVFGLQPSGSHLNGASPT
                     AAAPAALQPTRSLGTPPSKNDPLADFAAFGAPAPPRPPVMAEPAVVEPQPEHTVAVDA
                     TAAEPQAPIGRADAGAAAVLDPTVRLGEGEGEGEGED"
BASE COUNT          381 a          847 c          839 g          324 t
ORIGIN      
        1 cgcgagaccg gctccgcgaa ggtggtgtag gcgaaaccgg cgccgaggcc gaagcgcggc
       61 cgcgccccca gcgcccgcgc caccagcccg tggatcgcct cgtgcgccaa cggcacgacc
      121 agcgtcagca gcgccagccc accggcgatc gagggcaagc caagccggat gcgcgggcgc
      181 gacgtgccga gcacgcgggc tagcgcgacg aagagggcca gccagagggg cgcgagggcc
      241 agcgagagca gcgagacgcg ttccagcgga atccgctcct ggcgcgtcag gtggtagccg
      301 ggcgggccct ccacgctcac tgccgccagg ggagctggcc gccgcgcggc atggcgcgct
      361 ccagcgcgtg gacgtagtcg tcgagcgtcg ggtcggtcgg agcgagcagc cccggctcgc
      421 cgtcgccggt caccagcacg tcgagcgccc cgtcgagcag ccgggcgatg atgaagaagg
      481 ggaggttttg ccaggagtgc ggcgaggact cgccataggc gccgagcgcc gtctgcacca
      541 ggaggtcggc gtccgcggcc ggcatgcccg cgcggtagac cgagaacggc agcagcgcca
      601 gatcccaggc cggatcggtg acctgcgccc ggtcccagtc gaccacgccg ctgatctcgc
      661 cgtcctcgac cagcaggttg gcgggggtgt aatcgccgtg gcagaggcag agcgaggcgg
      721 tctcgatcat gtggcgcgag agccagttct gggcgctatc gaagagcggc ttgaggctgt
      781 ccggcgcctc gcggatgcgc tcgtcccagt ggctccacag cccctggatc tgccccagca
      841 cgatctcggt ctccggcccc gtgggatcgc tcatccaggg caccagcgcg cgccaatcga
      901 gcgcgtggat gcgcgccagc gtatgggcgt aggcggtggc ggcggccgcg atctcccagc
      961 gcgcctcgct gctctgcatg accggaaaga ggacgctacc ggggaggaag tccatcacga
     1021 gatagttggc cagatcgtcc ggctcgatgt ggagcaccgt ggggatgggc agcccgctgc
     1081 ccgccagcgc gcgcagcacg gccgactcgc ccgcctcgac ctggacctcg cgcttgacca
     1141 ccaagcgccg ccggcagacc tgctcgccgc tccgccactg cacatccagg gcgcgcacgt
     1201 gccacagcag gcggccgtcc agcgtctggt cgtcgagcac cgtgatggcc tcggcctccg
     1261 gcgagagctg gcgcacgacc ttgtgcacga tcgcttcgag tgggttccgc accggcgcgc
     1321 ctccttcttg gcatcccggc gccagcccta gcatctcccg taccgcgcct gcttgcctag
     1381 cagaatacgc acgcttcggc tatgcttagg gacagatgtc ctgtcgcttc aagtccaggg
     1441 gagatgagat gttcgagggc ctgttccagc caacacacct tatcatcatc ctcgtgctgg
     1501 tgctgatcgt cttcggcgcg gggaagcttc cggatgccgc tggcggcttc ggtcgcggca
     1561 ttcgcgagtt caagaagagc ctcaacgagg aggaggacga tcagacgacg gtgcagacca
     1621 cggtgaccgc gccgccggtc cccagccagt cggttccgac caccacgacc accatcacca
     1681 gcaacagcgc cactccgacc tgcgcgacct gcggcgcacc gattcccaac ggcgcgcact
     1741 tctgcgccaa ctgcgggagc aaagtggccg cctgatgccg gtcgctagtg gagtagcatc
     1801 atgaacttct tcggcatggg gccgctggag atcctcgcga tcacgctcgt ggccttcatc
     1861 atcttcggcc ccgagaagct gaccgagatc atgggcggtc tgggacgggc cgtgcgcgag
     1921 ttccgctcga tgagcagcga cctgaccggc gagttcgaac gcaccatcaa cgaggtgaag
     1981 gagacggccg gcgatctgca gagcacgacc cgcgacgccc tgacggtcga ggggctcaac
     2041 cccttcgacc agccggtctt cggcctgcag ccgtccggca gccacctgaa cggtgccagc
     2101 ccgaccgccg ccgcgccggc cgccctgcag ccgacgcgca gcctgggcac gccgccgtcc
     2161 aagaacgatc cgctggccga cttcgccgcc ttcggcgcgc cggccccgcc ccgtcccccg
     2221 gtgatggccg agccggccgt cgtcgagccg cagccggagc acacggtggc tgtggacgcg
     2281 accgcggccg agccacaggc acccatcggc agggccgacg ccggtgcggc cgccgtgctg
     2341 gatccgacgg tgcggctggg tgagggtgag ggtgagggtg agggtgagga t
//
LOCUS       DASUBI010000580         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_179029, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000580 DASUBI010000000
VERSION     DASUBI010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179029"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFU72_11250"
     CDS             complement(<1..1148)
                     /locus_tag="VFU72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5424108.1"
                     /translation="MNRSLVPIPSTAVIEAKPPKVYRTELTPVSFLRRSAAVFPDKTA
                     VIHGDDGRSFTYQEFAARVDRFASALRLAGMEQGDRVAFLCPNTPALLEAHFAVPAAG
                     GVLVPINTRLAAAEIAAIVRHAGARFLFVDHEFAALVASLNDPQPLVVRIDDSGRPDD
                     PYKQFLASGSPHPSDSWLTDEEEAISLCYTSGTTGKPKGVTYTHRGAYLNALGNVIET
                     GLNAESVFLWTQAMFHCNGWCFPWAVAAAAGAQVCLRKIEPALVWDLFAAYGVTHYNG
                     APTVHTMLANHPAAHRLAHPVTVTIGGAPPSPTLLRRLRELNFRPVHVYGLTETYAPI
                     TVCDWHSAWAALPEEAQAQLLARQGQGRVVAEPVRVVDETMTDVPRDGR"
     gene            complement(1287..>2355)
                     /locus_tag="VFU72_11255"
     CDS             complement(1287..>2355)
                     /locus_tag="VFU72_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424109.1"
                     /translation="FHTHLAEGRYWLTRALAADADAPPAVRARALHGAGQMAHWQGDD
                     AQATPLFEAALALWREVGDRRGAALALMLLGIVNEDQGKYAAARPFLEEAAALYQAEG
                     ETAWATLAGAYHLGTVAFGEGDYPQAQILLEEALRRFQAVGNRWGVGISLYSLGLLAY
                     QRGDPQGAARRYGECLAIVPESRSTESAITCLAGVAALATAAHPERAARLFGVAAALA
                     EPLGVTFTLAERLVFEQAKQTTRSALGDSRFAAAYHAMRNVTLEQVIAEGDAVLTLLC
                     ADCQGASQHAVNPADGPALTARELDVLRLIVAGRRDQEIADALFLSRRTVQTHVTHLF
                     AKLRVNTRAEAAACAVRRGLV"
BASE COUNT          380 a          797 c          826 g          352 t
ORIGIN      
        1 cgcccgtcac gtggcacgtc ggtcatcgtc tcgtcgacga cccgcaccgg ctccgcgacc
       61 acccgtccct gcccttgtcg cgccaacaac tgcgcttgcg cttcttcggg aagggccgcc
      121 catgccgaat gccagtcgca aaccgtgatc ggcgcatacg tctccgtcag cccgtagacg
      181 tggaccggcc ggaaattcag ctctcgcaga cggcgcagca acgtcggcga ggggggagcg
      241 ccgccgatgg tgaccgtgac ggggtgcgcc agccggtggg cggcggggtg gttggccaac
      301 atcgtgtgga ccgtcggcgc tccgttgtag tgggtcacgc cgtacgccgc aaagaggtcc
      361 cagacgaggg caggctcgat cttccgcaaa cagacctggg cgcccgccgc cgcggccacc
      421 gcccagggga agcaccagcc gttgcagtga aacatcgctt gcgtccagag aaaaacgctc
      481 tcggcgttca ggccagtctc gatgacgttg ccgagggcgt tgaggtaggc gccgcggtgg
      541 gtgtacgtta cgcccttcgg tttcccagtc gtccctgacg tgtagcacag cgaaatcgcc
      601 tcctcttcgt cggtcagcca ggaatccgac ggatgggggg aaccgctggc caggaactgc
      661 ttatacggat cgtccggcct gccggaatcg tcgatccgca ccacgagcgg ctgcgggtca
      721 ttcagcgatg caacgagcgc cgcgaattcg tggtcgacga agaggaagcg ggcgccggca
      781 tgccgcacga tggccgcgat ctccgcggcg gccagccggg tgttgatggg aaccaaaacg
      841 ccgcccgcgg ccgggacggc gaagtgggct tcgagcaggg ccggcgtgtt cgggcagagg
      901 aaggcgaccc ggtcgccctg ctccattccg gccagtcgca gggccgaggc gaagcggtca
      961 acgcgggccg caaactcctg ataggtgaag gaacgcccgt cgtcgccatg aatgacggcc
     1021 gtcttgtcgg gaaagaccgc cgcgctgcgg cgcaaaaagc tcacgggggt gagttccgtc
     1081 cggtagacct tcgggggctt ggcttcgatg acggccgtcg atgggattgg cacgagcgac
     1141 cggttcatgg gaatcctccg tccttgcgcg caacctccag atgcgcgctc atcgtgcgcg
     1201 ctcgccgcgc cgggcgcatc cggaaaaccg cgcgatcggc ggcagcgggc gatctacgta
     1261 cgttgcgcag tgccgcgggg cgcccgtcag acgaggccgc ggcggaccgc gcaggcggcg
     1321 gcctccgcgc gggtattcac ccggagcttg gcaaagaggt gcgtgacatg ggtctggacc
     1381 gtgcgccggc tgaggaagag cgcgtccgcg atctcctggt cgcggcgtcc ggcgacgatc
     1441 aggcgcaaca cgtccagttc gcgggcggtg agcgcgggac catccgctgg attgaccgcg
     1501 tgctgcgacg cgccttggca atcggcgcat aggagcgtca ggaccgcgtc gccttcggca
     1561 atgacctgct ccaacgtgac gttccgcatc gcgtggtagg cggcggcaaa gcgggagtca
     1621 cccaaggccg aacgcgtggt ctgcttggcc tgctcgaaca cgagccgctc ggccagcgtg
     1681 aatgtgacgc cgaggggctc cgccagcgcc gccgcgaccc cgaacaggcg ggcggcccgc
     1741 tctggatggg cagccgtcgc gagggccgcc acgccggcga gacaggtgat cgcggactcg
     1801 gtggaccggc tttccggcac tatggcgaga cattccccgt agcggcgggc cgctccctgt
     1861 gggtcaccgc gctggtatgc gaggaggcca agagaataca gcgagatgcc caccccccac
     1921 cgattgccga ccgcctgaaa tcggcgcaac gcttcctcca gcaggatctg cgcctgcggg
     1981 tagtcgcctt ccccgaatgc cacggtccca aggtggtacg cgccggcgag ggtggcccag
     2041 gccgtctccc cctctgcttg atagagggcg gctgcttctt caaggaacgg gcgtgccgcc
     2101 gcgtattttc cctgatcttc gttgacgatc ccaagcaaca tcagcgccag cgcggcgccg
     2161 cggcggtcgc ccacctcccg ccagagcgcc agggccgcct cgaacagggg cgtcgcctgg
     2221 gcgtcatcgc cttgccaatg cgccatctgg cccgccccgt gcagcgcccg ggcgcggacc
     2281 gccggcggcg cgtccgcgtc cgccgccagc gcacgcgtca accagtaacg tccttcggca
     2341 agatgggtgt ggaaa
//
LOCUS       DASUBI010000581         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_780672, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000581 DASUBI010000000
VERSION     DASUBI010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780672"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..803)
                     /locus_tag="VFU72_11260"
     CDS             complement(93..803)
                     /locus_tag="VFU72_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424110.1"
                     /translation="MSKFLFPGFASLSEFIAGRNGDCGPDALLMCLHVRDEGHWPLTA
                     AGLRALLDDMAARGCLMADNGMTNIPGLDRYLTLIGVPHHTTGYGAFTLSGLHEQLKS
                     LAGPSPKLVLVEWSAAGAGLHDDEPGVKFHFSAFGGIYMDGPASGWGYYRGDGDSRTD
                     DRGGKPSPAIVTGWPSIAAAKPIGYIVIEPAPPPAPKPDPRDAEIAALKGQVADLGGR
                     LSALEAGVTAAVATAEKK"
     gene            complement(800..1294)
                     /locus_tag="VFU72_11265"
     CDS             complement(800..1294)
                     /locus_tag="VFU72_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU5424111.1"
                     /translation="MSTIRERLRVLVGLLAAELVGRRRETAHDRAWGAHPAVATGDEL
                     TAGQRAADMMRSGLGTWTFLGVLAGCMALWMVTGGFGRDPAPFILLNLCLSTLAGVQA
                     AVLLIAAKRSDQVAAATALHTLQNTEKLAALIEQNTALTEQVAQLTREVHAAQLATRK
                     KTSA"
     gene            complement(1291..1836)
                     /locus_tag="VFU72_11270"
     CDS             complement(1291..1836)
                     /locus_tag="VFU72_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424112.1"
                     /translation="MSDTNALAHVHETSEAVATVELTPPHPPRVETPAYRAAHSFLIN
                     RKNAPCHVCGVTKRTLKNRAKNPCGATALETHHYPIERSLMDACDPMKVHADFPQVHD
                     RATLASFVDSIPNLLVLCDVHHRSLTHGIHHLLTQDFAVQRYLLDGYVVAAVAKDAAA
                     TLTADEQIEEKVGMEREAMSA"
     gene            complement(1853..2335)
                     /locus_tag="VFU72_11275"
     CDS             complement(1853..2335)
                     /locus_tag="VFU72_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424113.1"
                     /translation="MGAAAAARAGGGGGGGGGAQAEFAWGGQGNESEYERRQQMEAQR
                     YALQMFNEWRSHVDARLTSLENSSSVVAQTLARLEANMPSRADVEAASRTRLDIGTYT
                     VAHQALIDRVSRLESGPQRLVSWIALMVSGGIGCLMAMISGLALLVSIAGVLIAFTHP
                     "
     gene            complement(2405..3916)
                     /locus_tag="VFU72_11280"
     CDS             complement(2405..3916)
                     /locus_tag="VFU72_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424114.1"
                     /translation="MYCYTAPTNTNYLQPIVREYSAQIEALRVTTVNPGGYGQMSCLL
                     KVPDPRQPRPELAMFSRVVLTDGGDWWWIGEISDPRIGMRNDSEYLQIGALGLGSTLR
                     DDPIGISYTNQTVQQVAQNLISTRNYVNGTFKTQRVDSDTGGLFPTNPAGTHSPVYDG
                     NTFEEIFGELCLIASTSSNFYTWWTAAHPTHVDIAGFPQLQVWADVRDTATTGWMASV
                     ALGEVLEYEIAPSQERAYNYIEVLYNNGASGVGIKHSIDSRLNQTDFSQQTAPFPWRK
                     LRRDVSGTSTVGPSEAQAIADAYLAQYKDPSNKVHIVLMAVRDGYGNPQPLWRLTTPG
                     NQNIFVPEMAVRGQTLPTAPTAGVNQFYIVSAEYSESSTDDARVTLECDNFEDKASTR
                     IARLTAAADALLRSRAISGHVRQKAGAIETGQCGVSFNASLGGLHGGQSVNFKTQMTS
                     VPSAGQISLTQIDNVNASNPTISDITAVGFHLRVTSVASGDTIYVATYTVTSS"
     gene            complement(3965..>5973)
                     /locus_tag="VFU72_11285"
     CDS             complement(3965..>5973)
                     /locus_tag="VFU72_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424115.1"
                     /translation="ARFYLHRPDANNDLELTLTPSALTLNQRVGGALHQIATASVPFL
                     VHEAWYWVQVTQFPTQGTNAPYLIATLLYDTNGAIGSQIAQIAGSTFDAVTALSGVSG
                     FGPDGAALVVGGVSSGAGVRTYLFGPGGWLTTSGGTGTIAFGWEQDTTKTATPGTLDS
                     NGAVVVPVTSFGAARMDFPPAGTYDGAWVTYKGGAPSGTTAIPATVGQVFGLKALAKS
                     SGLDATASTRFWVQEWDASGTQVATTDGIGAVSGNLASWTALHATYTVTNASCAYISV
                     AVRSGSSNAGSAGATVWFDNVQCWNQTRTGQTTMSYHELRFPNNPCQLLLSGLQGDVA
                     APVALSLGLYISGTWAASSAIKLLLGRRQVAQAGAQLLGQAFIFSQQTGVLASTVYGG
                     YYVSFANMSDWQPLCLSQRPADALGTYHLLGLAQSALSAGNLANLKVRTRIYQSLARW
                     ATAAGLPGDLSEYLAPYITQGAGGMTITAQNLWTLLDAGQAPIPSFAQGALNDPTGTY
                     FTPTHEFTDTNVSHATTLVNWSALLPTDGALLTATLNNPTNSIAAPAAQWLWTYLDGL
                     YQPFTATQGGQASPGWTYSVESGPTANASSGAGGPGTTGQGTINVNLAADPLLTVDPT
                     VDVSGVSGARGVNQLVVVANDATGGNTWALHAELQYSPLYLFPR"
BASE COUNT          885 a         2322 c         1739 g         1027 t
ORIGIN      
        1 aacgtcacca cagccgtcag cgcggccgct tgaaccaaca caccatccat ctccgccacc
       61 ccacttctcg accttctcag cgcgttacag cgctacttct tctcagccgt tgccaccgcc
      121 gccgtcacgc ccgcctccag cgcgctcaat cgcccgccca ggtccgccac ttgccccttg
      181 agcgcggcga tctccgcgtc cctcggatct ggcttcggcg ctggcggcgg agcaggctcg
      241 atgacgatgt agccgatggg cttggcggcg gcgatgctcg gccagcccgt cacaatcgcc
      301 gggctcggct tgcccccgcg atcatccgtc cgcgagtccc cgtcgccgcg gtagtacccc
      361 cagccgctcg ccgggccatc catgtagatg ccgccgaacg cgctgaagtg gaacttgacc
      421 cccggctcgt cgtcgtgcag acccgcaccc gcagcagacc actccaccag caccagcttg
      481 ggcgacggcc ccgccaggct tttcagctgc tcgtgcaagc ctgagagcgt gaacgcgcca
      541 taccccgtcg tgtgatgcgg cacgccgatc agcgtcagat agcggtcgag gcccggaatg
      601 ttggtcatgc cgttgtctgc catcaggcag ccccgcgccg ccatgtcgtc cagcagcgcg
      661 cgcagccccg ccgccgtgag cggccagtgg ccctcgtcac gcacgtgcag gcacatcagc
      721 agcgcgtccg gcccacaatc gccattgcgc ccagcgatga attctgaaag actggcgaac
      781 cccggaaaca gaaacttgct catgcactcg tcttctttct cgtcgccagc tgcgcggcat
      841 gcacctcacg cgtcagctgc gccacctgct ccgtcagcgc cgtgttctgt tcgatcagcg
      901 cggccagctt ctcggtgttc tgtagcgtgt gcagcgccgt cgccgccgcc acctgatcac
      961 tgcgcttcgc cgcgatcagc agcaccgccg cctgcacccc cgccagcgtc gagaggcaca
     1021 gattcagcag gatgaacggc gccgggtcgc gcccgaagcc gcccgtcacc atccacagcg
     1081 ccatgcagcc agccaggacg cccaggaacg tccacgtccc cagcccgctg cgcatcatgt
     1141 ccgccgctcg ctgccccgcc gtcagctcgt cacccgtcgc caccgccgga tgcgcccccc
     1201 acgcccgatc gtgcgccgtc tctctccgcc tgcccaccag ctccgcggcc agcaacccaa
     1261 ccagcacccg cagccgctcg cgtatcgtgc tcatgcgctc atcgcctccc gctccattcc
     1321 caccttctcc tcgatctgct cgtccgccgt cagcgtcgcc gccgcgtcct tcgccaccgc
     1381 cgccaccaca tagccatcca gcaggtagcg ctgcaccgcg aagtcctgcg tcagcagatg
     1441 atggatgcca tgtgtgaggc tgcgatgatg cacgtcgcac agcaccagca gattcgggat
     1501 cgagtccacg aagctcgcca gcgtcgcgcg atcatgcacc tgcgggaagt ccgcatgcac
     1561 cttcatcggg tcgcacgcgt ccatcagact ccgctcgatg ggatagtgat gcgtctccag
     1621 cgccgtcgcc ccacatggat tcttcgcccg attcttcagc gtgcgcttcg tcaccccgca
     1681 cacatggcac ggcgcattct tgcggttgat caggaagctg tgcgccgccc gatacgccgg
     1741 cgtctccacc cgtggcggat gcggtggcgt cagctccacc gtcgccaccg cctcactcgt
     1801 ctcgtgcacg tgcgccaatg cgttcgtgtc gctcatagct atctcttccc ctctacggat
     1861 gcgtgaacgc gatcagcacc cccgcgatcg agaccagcag cgccaggccg ctaatcatcg
     1921 ccatcaggca gccgatcccc ccgctcacca tcagcgcgat ccacgagacc agccgctgcg
     1981 gcccgctctc cagccgtgag acgcgatcaa tcagcgcctg atgcgcgacg gtgtacgtgc
     2041 cgatgtccag ccgcgtgcgc gacgccgcct ccacatccgc cctgctcggc atgttggcct
     2101 ccagccgcgc cagcgtctgc gccaccaccg acgaggagtt ctccaggctc gtcaacctgg
     2161 cgtccacgtg tgaccgccac tcgttgaaca tctgcaacgc gtaccgctgc gcctccatct
     2221 gctgtcgccg ctcgtactcg ctctcgttac cctgccctcc ccaggcgaac tctgcctgcg
     2281 cgccaccgcc accgccaccg ccaccgcccg ctcgcgctgc tgctgctgcc cccaccgcac
     2341 taccctctcc ctgctcgtcc ctcgtccctc gtccctcgtc cctgacccct gctcgtcgca
     2401 gacactagct gctcgtcacc gtgtaggtcg ccacatagat cgtgtccccg ctcgccacac
     2461 tcgtcacccg cagatggaat cccaccgccg tgatatccga gatcgtcgga ttgctcgcgt
     2521 tgacgttgtc aatctgcgtg agcgatatct gacccgccga cggcacgctc gtcatctgcg
     2581 tcttgaagtt gaccgactgc cccccgtgca ggccgcccag gctcgcgttg aacgagaccc
     2641 cgcactgccc cgtctcgatc gcgcccgcct tctgccgcac gtgcccgctg atcgcccggc
     2701 tccgcagcag cgcatccgcc gccgccgtca gccgcgcaat ccgcgtgctc gccttgtcct
     2761 cgaagttgtc acactccagc gtcacccgcg catcatcggt gctgctctcg ctatactccg
     2821 cgctgacgat gtagaactga ttgactcccg ccgtcggcgc cgtcggcagc gtctgcccgc
     2881 gcaccgccat ctccggcacg aagatgtttt gattgcccgg cgtcgtcagc cgccacagcg
     2941 gctgcggatt cccatagccg tcgcgcaccg ccatcagcac aatgtgcacc ttgttcgacg
     3001 ggtccttgta ctgcgcgagg taggcgtcgg cgatggcctg cgcctcgctc ggccccaccg
     3061 tgctcgtccc gctcacgtcg cgccgcagct ttcgccaggg gaacggcgcc gtctgctgcg
     3121 agaagtcagt ctgattgagc cgcgagtcaa tcgagtgctt gatgccgacg ccgctcgcgc
     3181 cgttgttgta gagcacctcg atgtagttgt acgcccgctc ctggctcggc gcgatctcgt
     3241 actccagcac ctcgcccagc gccacgctcg ccatccagcc cgtcgtcgcc gtgtcgcgca
     3301 catccgccca cacctgcagc tgcgggaacc ccgcgatatc tacatgcgtc gggtgcgccg
     3361 ccgtccacca cgtgtagaag ttgctggagg tgctagcgat caggcacagc tcgccgaaga
     3421 tctcctcgaa cgtgttgccg tcgtagaccg gcgagtgtgt ccccgccgga ttggtcggga
     3481 agaggccgcc cgtgtcggag tccacccgct gcgtcttgaa ggtgccgttg acgtagttgc
     3541 gcgtcgagat caggttctgc gcgacctgct gcaccgtctg attcgtgtag ctgatgccga
     3601 tcggatcgtc gcgcagcgtg ctgcccagcc ccagcgcgcc gatctgcaag tactccgagt
     3661 cgttgcgcat cccgatccgc gggtcgctga tctcgccgat ccaccaccag tccccgccat
     3721 ccgtcagcac gacgcgcgag aacatcgcca gctccggccg cggctgccgt gggtccggca
     3781 ccttcagcag acacgacatc tgcccgtacc cgccagggtt gaccgtggtg acacgcagcg
     3841 cctcgatctg cgcggagtac tcgcgcacga tcggctgcag atagttggtg ttcgtcggcg
     3901 ccgtatagca atacatcgcg agaccagagc ggagtgccat aggtcgtgtt acctcacttc
     3961 accgtcagcg cgggaacaga tagagcggcg agtattgcag ctcggcgtgc agcgcccagg
     4021 tgttaccccc cgtcgcgtcg ttggcgacaa cgaccagctg attgacgccg cgcgccccgc
     4081 tcacgccact cacgtccacc gtcgggtcca ccgtcagcag cggatcagcc gccagattga
     4141 cgttgatcgt gccctgcccc gtcgtccccg gcccgcccgc gccgctgctc gcattcgccg
     4201 tcggcccgct ctccaccgag taggtccacc ccgggctcgc ctgcccgccc tgcgtcgccg
     4261 tgaacggctg gtacagcccg tccagatagg tccacagcca ctgcgccgcc ggcgcggcga
     4321 tgctgttggt cgggttgttg agcgtggccg tgagcagcgc gccgtcggtt ggcagcagcg
     4381 ccgaccagtt gaccagcgtg gtcgcgtgcg agacgttggt gtcggtgaac tcgtgtgtcg
     4441 gcgtgaagta ggtgccagtg gggtcgttca gcgcgccctg cgcaaaggac gggatgggcg
     4501 cctgccccgc gtccagcaac gtccacaggt tctgcgccgt gatcgtcatg ccgcccgcgc
     4561 cctgcgtgat gtaaggtgcg agatactccg agaggtcgcc aggcagcccc gccgccgtcg
     4621 cccagcgcgc cagcgattga tagatgcgcg tgcgcacctt caggttcgcc agattgcccg
     4681 cggagagggc cgactgcgcc aggccgagca agtggtacgt ccccagcgcg tccgccggcc
     4741 gctgcgagag gcacagcggc tgccagtcgc tcatgttggc gaagctgacg taatagccgc
     4801 cgtagaccgt gctcgccagc acgccggtct gctgagagaa gatgaaggcc tgccccagca
     4861 gctgcgcgcc cgcctgcgcc acctgtcgcc gccccagcag cagcttgatc gccgagctcg
     4921 ccgcccacgt cccgctgatg tagagcccca gcgagagcgc caccggcgcc gccacatccc
     4981 cctgtagccc gctcaagagc agttggcacg gattgttggg gaagcgcagc tcgtggtagc
     5041 tcatcgtggt ctggccggtc cgcgtctgat tccagcactg cacgttatcg aaccagaccg
     5101 tcgcacccgc gctccccgcg ttgctgctcc ccgaccgcac cgccaccgag atatacgcgc
     5161 agctcgcgtt tgtgaccgtg tacgtcgcgt gcagggcagt ccaactcgcc agattcccgc
     5221 tcaccgcgcc gatgccgtcc gtcgtggcga cctgcgtccc gctggcgtcc cactcctgca
     5281 cccagaagcg cgtgctcgcc gtcgcatcca gcccgctgct cttggccagc gccttcagcc
     5341 cgaagacctg gccgaccgtc gccgggatcg ccgtcgtccc gctcggtgcc ccgcccttgt
     5401 acgtcaccca cgcgccgtca tacgtccccg ccggcgggaa gtccatgcgc gcggccccga
     5461 agctcgtcac cggcaccacc accgcgccgt tgctgtccag cgtgcccggc gtggccgtct
     5521 tcgtcgtgtc ctgctcccag ccgaaggcga tcgtgccggt cccgccgctg gtcgtcagcc
     5581 agccaccggg tccgaagaga tacgtccgca cccccgcgcc gctgctcacc ccgccgacca
     5641 ccagcgccgc gccgtccggc ccgaagccgc tgacacccga gagcgccgtc accgcgtcga
     5701 aggtgctgcc cgcgatctgc gcgatctggc tgccgatggc gccgttggtg tcgtagagca
     5761 gcgtggcgat caggtacggc gcgttggtgc cctgcgtcgg gaactgcgtc acctgcaccc
     5821 agtaccacgc ctcatgcacc agaaacggca cgctcgccgt cgcgatctga tgcagcgccc
     5881 cccccacccg ctgattgagc gtcagcgcgg agggcgtcag cgtcagctcc aggtcgttgt
     5941 tcgcgtccgg gcggtgcaga tagaaccgcg ccg
//
LOCUS       DASUBI010000582         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_941871, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000582 DASUBI010000000
VERSION     DASUBI010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941871"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..2869
                     /gene="secA"
                     /locus_tag="VFU72_11290"
     CDS             263..2869
                     /gene="secA"
                     /locus_tag="VFU72_11290"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5424116.1"
                     /translation="MRKFFTKVLGDPNQRVLNDLRETVAEINALEPEFQQLSDDKLRG
                     KTAEFKGRLANGETLDDILPEAFAAVREAAKRALGQRHYDVQLMAGIVLHDGKIAEMK
                     TGEGKTLVATLPLYLNSLTGQGVHLVTPNDYLSRIGGGWMGPVYHQLGVSVAVIFHED
                     AAIYDPDYTDADVSPDDRLNHWRSIPRREAYAADITYGTNHEFGFDYLRDNLALMKEQ
                     QVQRPLNYAIVDEVDNILIDEARTPLIISGQSRETVDRYYQFAKLVRQLRPGVHYEID
                     IKDRVVTLTDDGMDKVERLLAIPEGQSLYDDRYSEMTAFLEQALKAQALFQRDKDYIV
                     RDGEIIIVDEFTGRMMLGRRYSEGLHQAIEAKENVRVQEETVTQATITFQNFFRMYEK
                     LAGMTGTAETEAEEFATIYNLEVVVIPTNRPMIRSDFSDQVYRDEDAKFQAVVREVQE
                     MTRQGRPVLVGTTSVEKSERLSEMLNRAGVKHSVLNAKQHEREAAIVAEAGQPAAVTI
                     ATNMAGRGTDIILGRSVAEWGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFFLS
                     LEDELLLRFGSTRISGLMDRLGMDDETPIEHNLITKTIENAQTKVEGHNFDIRKHLVE
                     YDDVVNRHREVIYDQRQKILDGEDMKEKVLDMIEHQIEVLVAAHWPESRHEEPEYQEI
                     LDSFKGVVGPEIGLSVRDIADRPQEEVVDLLSEKSEELYDRVEAENGHDEQRMLEHLV
                     LLQIIDHLWVEHLTEMDHMRTGVGLQAYGQRDPLVEYKTEGFRMFQQLLQNIEYDFVH
                     RIYAARLARVPQQQTRPIGQTNHPAEAGPVRRKAKEKVGRNDPCPCGSGKKYKQCHGA
                     PSAANKVAGARG"
BASE COUNT          582 a          941 c          952 g          430 t
ORIGIN      
        1 caacggaaga atctcctctc ggttgcggca gcactgtcgc ttgctgctga tgggagaagc
       61 ttcggctgcg gcctcagcat gacaggcgtg gggcgtaggc taacagggtg atggctcgac
      121 gggccagcgg cggcccctgg ccgcagacgg gcgctcaacg aagcggttgc gcgctgcaat
      181 gtttgacgca gacgggtaaa attgggggac ggccagagag ctgaccgcat ctcgtctgca
      241 tctgcccacg ggggatttca tcatgcgaaa gttttttacg aaagttctcg gagaccccaa
      301 ccagcgcgtg ctcaacgatc tccgggagac ggtcgccgag atcaacgcgc tcgagcccga
      361 gttccagcag ctcagcgacg acaagctccg gggcaagacg gcggagttca agggccggct
      421 ggccaacggc gagacgctcg acgacattct gcccgaggcc ttcgcggccg tacgtgaggc
      481 cgccaagcgc gcgctcgggc agcgccacta cgacgtacag ttgatggccg gcatcgtcct
      541 gcacgacggc aagatcgccg agatgaagac cggcgagggc aagacgctgg tggcgaccct
      601 gccgctctac ctcaactcgc tcaccggcca gggcgtgcat ctggtcacgc cgaatgacta
      661 cctgtcgcgc atcggcggcg gctggatggg cccggtctac catcagctcg gcgtctcggt
      721 cgcggtgatc ttccacgaag acgcggccat ctacgatcct gactacaccg atgccgacgt
      781 cagccccgac gaccggctga accactggcg ttcgatcccg cgccgcgagg cctacgccgc
      841 cgacatcacc tacggcacca accacgagtt cggcttcgac tacctgcgcg acaacctggc
      901 tctgatgaag gagcagcagg tccaacgccc gctgaactac gccatcgtcg acgaagtcga
      961 caatatcctg atcgacgagg cgcgcacgcc gctgatcatc tccggccagt cgcgcgagac
     1021 ggtcgaccgc tactatcagt tcgccaagct ggtgcgccag ttgcgcccgg gcgtccatta
     1081 cgagatcgat atcaaggacc gggtcgtcac cctgactgat gacggtatgg acaaggtcga
     1141 gcgtctgctg gcgatccccg aagggcagag cctctacgac gaccgctact ccgagatgac
     1201 cgcgttcctg gagcaggcgc tcaaggcgca ggccctcttc cagcgcgaca aggactacat
     1261 cgtccgcgac ggcgagatca tcatcgtgga cgagttcacc ggccgcatga tgctcggacg
     1321 gcgctatagc gaagggctgc accaggcgat cgaggccaag gagaacgtgc gggtccagga
     1381 ggagacggtc acgcaggcga ccatcacctt ccagaacttc ttccgcatgt acgagaagct
     1441 ggccggcatg accggcacgg ctgagaccga ggccgaggag ttcgccacca tctacaacct
     1501 cgaagtagtg gtcatcccga ccaaccggcc gatgatccgc tcggattttt ccgaccaggt
     1561 ctaccgcgac gaggacgcca agttccaggc ggtggtgcgc gaggtccagg agatgacccg
     1621 ccagggccgg ccggtcctgg tcggcacgac ctcggtggag aagagcgagc ggctcagtga
     1681 gatgctcaac cgggccggcg tcaagcactc ggtgctgaac gccaagcagc acgagcgcga
     1741 ggccgccatc gtggccgagg ccgggcagcc ggcggcggtg acgatcgcga ccaacatggc
     1801 tggccgcggc accgacatca tcctcgggcg cagcgtggcc gagtggggcg ggctgcacat
     1861 catcggcacc gagcggcacg aatcgcgccg catcgacaac cagctccgcg gccgtgccgg
     1921 ccggcagggt gacccgggct ccagccgctt cttcctgtcg ctggaggacg agctgctgct
     1981 gcgcttcggc tcgacgcgta tcagcggctt gatggaccgc ctcggcatgg acgacgagac
     2041 gccgatcgag cacaacctga ttaccaagac gatcgagaac gcccagacca aggtcgaggg
     2101 gcacaacttc gacatccgca agcacctggt cgagtacgac gacgtggtca accggcaccg
     2161 tgaggtgatc tacgaccagc gccagaagat cctcgacggc gaggacatga aggagaaggt
     2221 gctcgacatg atcgagcatc agatcgaggt gctggtggcg gcgcactggc cggagtcgcg
     2281 ccacgaggag ccggagtatc aagagattct cgactcgttc aagggggtcg tcgggccgga
     2341 gattggcctg agcgtgcgcg acatcgccga ccgcccgcag gaagaagtcg tcgacctcct
     2401 gtccgagaaa tcggaggagc tgtacgaccg ggtcgaggcg gagaacggcc acgacgagca
     2461 gcgcatgctc gagcacctgg tgctcttgca gatcatcgac catctctggg tcgagcacct
     2521 gaccgagatg gatcacatgc gcaccggcgt cggcctgcag gcctacggcc agcgcgatcc
     2581 attggtcgag tacaagaccg agggcttccg catgttccag caactgctgc agaacatcga
     2641 gtacgacttc gtccaccgca tctacgctgc tcggctggcg cgcgtgccgc agcagcagac
     2701 tcgcccgatc gggcagacga accacccggc cgaggcgggg ccggtgcggc gcaaggccaa
     2761 ggagaaggtc gggcgcaacg acccctgccc ctgcggcagc ggcaagaagt acaagcaatg
     2821 ccacggcgcg ccctccgccg ccaacaaggt tgccggtgcg cgggggtagg tttcgctcgc
     2881 tctgggcatc ctcaacacga accct
//
LOCUS       DASUBI010000583         5356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1105347, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000583 DASUBI010000000
VERSION     DASUBI010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105347"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFU72_11295"
     CDS             <1..402
                     /locus_tag="VFU72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HEU5424117.1"
                     /translation="LDRHGAADLETTPTRDARLEPEDGLLAHSNHYVAAALQGEERSK
                     DPGLSNSRVRLRRMRDLLAERRGELNASAMQEVLRDRACYPDTICRMPGDAVSDVITF
                     ASVIAAPSAGELWVAVGPPNEHGYQHHAFSM"
     gene            715..951
                     /locus_tag="VFU72_11300"
     CDS             715..951
                     /locus_tag="VFU72_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424118.1"
                     /translation="MKQPISVSDVATVLRLAYGYGVFWTWQEHGLVLIEPCHPADAHL
                     VEEAASYLRSLGAHAYATEGEANRIVVTGSGERP"
     gene            1079..1444
                     /locus_tag="VFU72_11305"
     CDS             1079..1444
                     /locus_tag="VFU72_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEU5424119.1"
                     /translation="MKDERPGRADPDSRVRTHLANERTYLAWLRTGTAMVALGLASAQ
                     FLTHHLAPGFPLVRILAVTLVLTGIAITVTGGWRYLDNMRRIDEQKFQPAARSVILSA
                     SAVALVGLLAVVFIVLLHE"
     gene            1660..2529
                     /locus_tag="VFU72_11310"
     CDS             1660..2529
                     /locus_tag="VFU72_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424120.1"
                     /translation="MAYADIFEYPLTLPEIHRYLIGLAVSRETVGAAIANVLLPSGRL
                     VERDGYYALPGREAIVATRQERQERAAAVWPRALRYGRLIASLPFVRLVAITGELAMD
                     NVRPESDIDFFVVTEHRRLWLCRALILAIVHAAGRLGDTICPNYLISERALALDDGDL
                     YSAHEIAQMIPLAGLETYARFRQLNPWVYDFLPNAAAVPRDLRARPAARPARALAESA
                     LRSPAGAALERWESGRKLRRFRAEARGHAEASFTLDWCKGHLHDHGQLILEAFAKRAP
                     ALAGDDDEGWGES"
     gene            2526..4040
                     /locus_tag="VFU72_11315"
     CDS             2526..4040
                     /locus_tag="VFU72_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5424121.1"
                     /translation="MSEVLFGQSYYLHFDAKLFEAMQPYPPLGTLYAASFLRERGYDV
                     ALFDAMLASSEEEWRAALARERPRFAVLFEDNFNYLSKMCLLRMRQAAFTMLDMAREA
                     GCAVIVCGADASDHAAQYLAHGADYVIQGEGEATLGVLLDRLTGRGDAPLESILGLVF
                     NDPATGKLVCAPRRPDLRDLDALPFPAWDLVDIPRYQRIWRARHGYYSMNMVTTRGCP
                     FHCNWCAKPIWGQRYHARSPENVAAELSWLKQTYNPDHIWFVDDIMGLKPGWVERFAA
                     LVEEQDVRVPFKCLNRVDLLLRGDTIDALRRAGARTVWVGAESGSQKILDAMDKGTRV
                     EQIYEVTRRLHAAGIEVGFFLQFGYPGETRADIEQTLQMVRDCRPDDIGMSVSYPLPG
                     TTFHERVREQLGVKQNWLDSNDLAMLYRGPYSTEFYRQLHVVLHKEFRSHKYRAELGR
                     ALAGRAMRRANLRQAAGLAKCRLTLPLARRRLERLAAGGSDEQVAAAMNAAGGA"
     gene            4075..>5356
                     /locus_tag="VFU72_11320"
     CDS             4075..>5356
                     /locus_tag="VFU72_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5424122.1"
                     /translation="MHSYCLAEDPHELAVMKPYPPLGILYISSHLKARGFDVKIFDST
                     FSTAADFRAYVERERPPVVGFYANLITRSKVIRNARVCKELGCTVIAGGPEPANYPEE
                     YLAQGVDVVVVGEGERTLEELLPHLARYGPAGMQEIDGIVYREGDGQLVRTKPRAYLD
                     NLDLQPFPDREAIDLDQYVRAWREHHGMGSVSLITARGCPYRCAWCSHAVYGYTHRRR
                     TPENVADELELIIERYQPDMVWYADDVFTIHKGWFFRYADELRRRGIRIPFETISRED
                     RLNEDIVRTLAEIGSFRIWVGAESGSQRVLDAMNRRTKATRVEEVVHLLQRYGIEAGM
                     FIMLGYDGEERVDLEETVDLLKRSNPNQFLTTVAYPIKNTPYYNTVQDKVIPLAPWDT
                     GSDRDFTVAGRHSRRYYDLATRWMVNEVALHQERQ"
BASE COUNT          841 a         1879 c         1784 g          852 t
ORIGIN      
        1 ctcgaccgcc acggcgcggc cgacctggag acgacgccga cccgcgacgc tcgcctggag
       61 ccggaggacg gcctgctggc gcactcgaac cactacgtgg ctgctgcgct gcagggtgag
      121 gagcgctcta aggacccagg gctgagcaac tcgcgtgtcc gactacgacg catgcgcgac
      181 ctgctggccg agcggcgtgg agagctgaac gcgagcgcga tgcaggaggt gctgcgcgat
      241 cgcgcctgct accccgacac catctgccgc atgccgggcg acgccgtctc ggatgtgatc
      301 accttcgcgt cggtgatcgc cgcgccctcg gccggtgagc tgtgggtcgc ggtcggcccg
      361 cccaacgagc acggctacca gcaccacgcc ttcagtatgt gagcggaggt cggctgacga
      421 cttcgaattg tggcgtatgc ggacggatcc ctccactacg gtcgggatga cagtggaaag
      481 aaggggctgg cgaggaggat gagccgtgga cgatgatcgg tctcgttagc taggccgcga
      541 ccgttccctg tcatcccgac cgcagtggag ggatccgtct tcacgcgggt caaatgccga
      601 ggtcaggctg cgctgccgca cccggcacca cagcggaata gtcgcccggt gatagaattc
      661 gggctgaaac gtcggccccg gcgcttgccc gcccacgctg agagatgaat ttcgatgaag
      721 cagccgatct ccgtgagcga tgtcgccacc gtgctgcgcc tggcctacgg ctacggagtc
      781 ttctggacct ggcaggagca cggcctggtc ctgatcgagc cctgtcaccc ggccgatgcc
      841 cacctggtcg aggaagccgc cagctacctg cgctcgctcg gcgcccatgc ctacgccacc
      901 gagggcgagg ccaaccgcat cgttgtgacc ggaagcggcg agcgcccctg aggcgcgaca
      961 catcctccat tcctccgatc cctcgacgac cgtgagttgc cgcgcgtggc ctgtctcgtg
     1021 cacacgcgtc ccggcgacgg tcgacgtgtc gcggcggcga gaggacatgc gcttggacat
     1081 gaaagacgag cgtcccgggc gagccgaccc ggatagccgg gtgcgcaccc atctggcgaa
     1141 cgagcgtacc tacctggcct ggctgcgcac cggcacggcc atggttgccc tcgggctggc
     1201 gtcggcccag ttcctgacac accacctggc gccgggcttc ccgctggtgc gcatcctggc
     1261 ggtcacgctc gtcctcaccg ggatcgcgat caccgtcacc ggcggctggc gctacctgga
     1321 taacatgcgc cgcatcgacg agcagaagtt tcagcccgcc gcgcgctcgg tcatcctctc
     1381 cgccagcgct gtggccctgg tcggcctgct cgccgtcgtc ttcatcgtcc tcctgcacga
     1441 ataaccgccc ccgcccttgt atctggtctc gattccaact actatcttcc aatccatcgg
     1501 gcgcgtggcc gcgtagagtt ggcgaacggt ttcaggggtt agccgcacgt cgggaatccg
     1561 gtgaccatta tgagcgccat ccggtttgaa gcctcgtcac cctcgcggcc ggcagtagcc
     1621 ggttcaaacg atgcgctgag gggggccatc gtgcgcacgg tggcctacgc tgacatcttc
     1681 gagtacccgc tcacgctgcc cgagatccac cgctacctca ttggtctggc cgtctcgcgt
     1741 gagacggtgg gagccgccat cgcgaacgtg ctgctgccgt ccgggcggct cgtcgagcgg
     1801 gacggatact atgcgttgcc gggccgcgaa gccatcgtcg ccacccggca ggagcggcaa
     1861 gagcgcgccg cggccgtctg gccgcgggcg ctgcgctacg ggcggttgat cgccagcctt
     1921 cccttcgtgc gcctggtggc gatcaccggc gagctggcga tggataacgt gcgcccggag
     1981 tcggacatcg acttcttcgt ggtgaccgag caccggcgcc tctggctctg ccgagcgctg
     2041 atcctcgcta tcgtccacgc cgcgggccgc ctcggcgata cgatctgccc gaactacctg
     2101 atctcggagc gggcgctggc gctggacgac ggcgacctct acagcgcgca cgagatcgcc
     2161 cagatgatcc cgttggccgg gctggagacc tacgcgcgct ttcggcagct caacccctgg
     2221 gtctacgact tcctgcccaa cgcggcggcg gtgccgcgcg atctgcgtgc ccggcccgcc
     2281 gcccgtccgg cgcgtgcgct ggccgagagc gcgctgcgct cgccggccgg ggcggcgctg
     2341 gaacgctggg aaagtggacg caagctgcgc cgcttccggg ccgaggcgcg cggccacgcc
     2401 gaggcctcct tcacgctcga ctggtgcaag gggcacctcc acgaccacgg ccagctcatc
     2461 ctggaggcct tcgccaaacg cgccccggcg ctggccggcg acgacgatga gggctggggg
     2521 gagtcatgag cgaggttctc ttcggccagt cgtactacct gcacttcgac gccaagctct
     2581 tcgaagcgat gcagccgtat ccgccgctcg gcacgctcta cgcggcctca ttcctgcggg
     2641 agcgcggcta cgacgtggcc ctctttgacg ccatgctggc ctcctcagaa gaggagtggc
     2701 gcgcggcgct ggcgcgcgag cggccgcgct tcgccgtgct cttcgaggac aacttcaact
     2761 atctctcgaa gatgtgcctg ctgcgcatgc gccaggcggc cttcaccatg ctcgacatgg
     2821 cgcgcgaggc cggctgcgcc gtcatcgtct gcggcgccga cgcctccgac cacgccgccc
     2881 agtacctggc gcacggggcg gactatgtca tccagggcga gggcgaggcg acgctgggcg
     2941 tgctgctcga ccggctgacc ggccgcggcg acgccccgct ggagtcgatc ctgggcctcg
     3001 tcttcaatga tccggccaca ggcaagctgg tctgcgcgcc gcgccggccc gacctgcgcg
     3061 acctcgacgc gctccccttc cccgcctggg acctggtcga tatcccacgc taccagcgca
     3121 tctggcgcgc gcgccacggc tactactcga tgaacatggt caccacccgc ggctgtccct
     3181 tccactgtaa ctggtgcgcc aagccgatct ggggccagcg ctaccacgcg cgcagccccg
     3241 agaacgtggc cgccgagctg agctggctca agcagaccta caaccccgat cacatctggt
     3301 tcgtggacga catcatgggg ctgaaaccgg gctgggtgga gcgcttcgcc gcactcgtcg
     3361 aggagcagga cgtccgcgtc cccttcaagt gcctcaaccg ggtcgatcta ctgctgcgcg
     3421 gcgacaccat cgacgcgctg cgccgggcgg gcgcgcgcac ggtctgggtc ggggccgagt
     3481 ccggctcgca gaagattctc gacgccatgg acaagggtac gcgcgtcgag cagatctacg
     3541 aggtgacgcg gcggctgcac gcggccggga tcgaggtcgg tttcttcctg cagttcggtt
     3601 accccggcga gacgcgcgcg gacatcgagc agacgctcca gatggtgcgc gactgccggc
     3661 ccgacgacat cggcatgtcg gtctcctacc cgctgccggg caccaccttc cacgagcggg
     3721 tgcgtgagca gctcggcgtc aagcagaact ggctcgactc caacgacctg gccatgctct
     3781 accgcggccc gtactcgacc gagttctatc gccagctgca cgtggtgctg cacaaggagt
     3841 tccgctcgca caagtaccgc gcggagctgg gtcgcgcgct ggccggacgc gccatgcgcc
     3901 gggcgaatct gcggcaggcg gccggactgg ccaagtgtcg gctgacgctg ccgctggcgc
     3961 gacgccggct ggagcggctg gccgcgggtg gcagcgatga gcaggtggcg gcggcgatga
     4021 acgctgccgg cggcgcctga cgggagtagt gggggacaga tggatatcct actggtgcac
     4081 agctattgtc tggccgagga cccacacgag ctggcagtga tgaagcccta tccgccgctc
     4141 ggcatcctct acatcagctc gcacctgaaa gcgcggggct tcgacgtcaa gatcttcgat
     4201 tccaccttca gcactgccgc cgacttccgt gcctacgtcg agcgcgagcg cccgccggtg
     4261 gttggcttct acgccaatct gatcacccgc tccaaggtga tccgcaacgc gcgcgtctgc
     4321 aaggagctgg gctgcaccgt gatcgcgggc ggccccgagc cggccaacta cccggaggag
     4381 tacctggcgc agggcgtcga cgtggtcgtg gtcggcgagg gcgagcgcac gctggaggag
     4441 ttgctgccgc acctggcgcg ctacggcccg gccggcatgc aggagatcga cggcatcgtc
     4501 taccgcgagg gcgacggcca actggtgcgt accaagccgc gcgcctacct cgacaacctc
     4561 gacctacaac cctttcccga ccgcgaggcg atcgacctgg atcaatacgt gcgcgcctgg
     4621 cgcgagcacc acggcatggg ctcggtttcg ctgatcacgg cgcgcggctg cccctaccgc
     4681 tgcgcctggt gcagccacgc ggtctacggc tacacccatc gccgccgcac gccggagaac
     4741 gtggccgacg agctggagct gatcatcgag cgctaccagc cggacatggt ctggtacgcc
     4801 gacgacgtct tcaccatcca caagggctgg ttcttccgct acgccgacga gctgcgccgg
     4861 cgcggcatcc gtatcccctt cgagaccatc tcgcgcgagg accggctcaa cgaggatatc
     4921 gtgcgcacgc tggccgagat cggcagcttc cgcatctggg tgggggccga gagcggttcc
     4981 cagcgtgtgc tcgacgcgat gaaccgccgc accaaggcga cgcgcgtgga ggaggtcgtg
     5041 cacctcttgc agcgctatgg catcgaggcc ggcatgttca tcatgctcgg ctacgacggc
     5101 gaggagcgcg tggacctgga ggagacggtc gatctgctca agcgctccaa ccccaatcag
     5161 ttcctgacca ccgtggccta cccgatcaag aacacgccct actacaacac agtgcaagac
     5221 aaggtcatac cgctcgcgcc ctgggatacc ggctcggacc gcgacttcac cgtcgccggg
     5281 cggcactcgc ggcgctacta cgacctggcc acgcgctgga tggtcaacga ggtggcgctg
     5341 caccaggagc gccaga
//
LOCUS       DASUBI010000584         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1118968, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000584 DASUBI010000000
VERSION     DASUBI010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118968"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFU72_11325"
     CDS             <1..259
                     /locus_tag="VFU72_11325"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEU5424123.1"
                     /translation="CCAERRLILELDGAIHDEQKEYDSARTEHLEAYGYTVIRFHNDD
                     VLTNLSGVLARIASQALRLPSPRIGRGAGGEGPSEDTTLAW"
     gene            271..1287
                     /locus_tag="VFU72_11330"
     CDS             271..1287
                     /locus_tag="VFU72_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5424124.1"
                     /translation="MGNMLDGKVALVTGAGRGIGRGVALLLAQEGASVVVNDLGTSLD
                     GQGVDTGPAATVAEEIKQAGGKAVANTESVADFEAAHRMVQQAVDSFGKLDIVVNVAG
                     ILRDRMIFNMAPEEWQAVLDVHLKGTFNTCRWASVLFREQRSGRLINMSSTSAFGAPG
                     QPNYAAAKAGIIGLTLSCANGLTRYNVTANAILPSGSTRMIDSIPQRREQALKDSGKL
                     PSELAKGTERDPDNVAPLVAFLASDAAQNVNGMLFGSFGYNVALMSQPKIVKTIKSDE
                     RWTVDQLSEIIPKAFAPDLQEITNQRGFLDLIKSIPQDQWQQIEPGLRAWATELEPYG
                     ELTW"
     gene            1371..2525
                     /locus_tag="VFU72_11335"
     CDS             1371..2525
                     /locus_tag="VFU72_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEU5424125.1"
                     /translation="MASNLSGKAAIVGVAESDKIGVLPDKPAIMLHAEAARNALEDAG
                     LTKDDVDGLFSAGISTLEIGEYLGIEPKVTDTTAVGGSSFVIHIAHAATAIATGRCEV
                     ALVTHGESGRSRVGMPPRSMDPQSLRGQFEAPYGLPQPVGAYALACSRHMYEYGTTKA
                     QLAEIAVATRKWAMLNPKAMMREPITIEDVLNSRPISWPFNLLDCCLVTDAGGAYVMT
                     SVDRARDLKQTPAVVLGFGEGHDHAMISQMPSYTSFAARNSGKRAFEMAGVTHDELNL
                     AMIYDSFTYTVLLSLEELGFCAKGEGGQFVSNQRTAPGGEFPLNTNGGGLSYTHPGMY
                     GMFAVIEAVRQLRQDYADQGIRQVPDCKMALVHGTGGVLSSAGTAILARG"
     gene            2553..2981
                     /locus_tag="VFU72_11340"
     CDS             2553..2981
                     /locus_tag="VFU72_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEU5424126.1"
                     /translation="MSEQKQYVKPLPKSDGTTAPFWESVKAHAMQIQRDNKNGKYFFY
                     PRGLSPYGLHDDFSWQPVSGRGVVHAFSIVHNNRAPGFRDEIPYVVAMIELEEGARMM
                     SNLIDVQPDPEHVKIGMPVEVVYEDVTDEVTLPKFRPRQG"
BASE COUNT          567 a         1018 c         1072 g          464 t
ORIGIN      
        1 ctgttgcgcc gagcgtcggc tcatcctcga actcgacggg gccatccatg atgagcagaa
       61 agagtacgac agcgcacgca ccgaacacct cgaagcctac ggatataccg tgattcgctt
      121 ccacaatgat gacgtgttga ccaatctcag cggcgtcctt gcccggatcg catcccaagc
      181 tcttcgtctc ccctcgccca gaattgggag aggggccggg ggtgagggcc cgtccgagga
      241 cacaacacta gcttggtaaa ggaggtacca atgggcaaca tgctggatgg caaagtcgcg
      301 ctggtgaccg gcgcggggcg cgggatcggg cgcggggtgg cgctgctcct ggcgcaggag
      361 ggcgcctcgg tcgtggtcaa cgacctgggc accagcctcg acgggcaggg ggtcgatacc
      421 gggccggcgg ccacggtggc cgaggagatc aagcaggcgg gcggcaaggc ggtcgccaac
      481 accgagtcgg tggccgactt cgaggccgcg caccgcatgg tgcagcaggc ggtcgacagc
      541 ttcggcaagc tcgacatcgt ggtgaatgtg gccggcatcc tgcgcgaccg catgatcttc
      601 aacatggcgc cggaggagtg gcaggcggtc ctcgacgtgc acctcaaggg caccttcaac
      661 acctgccgct gggcctcggt cctcttccgc gagcagcgct ccgggcggct gatcaacatg
      721 tcctcgacct cggccttcgg cgcgccgggc cagccgaact acgccgccgc caaggccggc
      781 atcatcggcc tgacgttgag ctgcgccaac ggcctgacgc gctacaacgt taccgccaac
      841 gccatcctgc cgagcggctc gacccgcatg atcgactcga tcccgcagcg gcgcgagcaa
      901 gcgttgaagg attcgggcaa gctgccgagc gagctggcca aggggaccga gcgcgacccg
      961 gacaacgtgg cgccactggt cgccttcctg gccagcgacg ccgcccagaa cgtcaacggc
     1021 atgctcttcg gctccttcgg ttacaacgtg gcgctgatgt cgcagccgaa gatcgtcaag
     1081 acgatcaaga gcgacgagcg ctggaccgtc gatcaactga gcgagatcat cccgaaagcg
     1141 ttcgcgccgg acctgcagga gatcacgaac cagcgcggct tcctggatct gatcaagagc
     1201 attccgcagg accagtggca gcagatcgag ccggggctgc gcgcctgggc gacagagctg
     1261 gagccgtacg gcgagctgac gtggtaggcc ctcaccgggg tgccctcacc cccagcccct
     1321 ctcccgatgc gcgggagagg ggaggagagt agatgaagga aggttgcagg atggccagta
     1381 atctctccgg caaagccgcg atcgtcgggg tggcggagtc ggacaagatc ggggttctgc
     1441 cggataagcc ggcgatcatg ctgcacgccg aggcggcgcg caacgcgctg gaggacgccg
     1501 gcctgaccaa ggacgacgtc gacggcctct tcagcgccgg tatctcgacg ctggagatcg
     1561 gcgagtatct cggtatcgag ccgaaggtga ccgacaccac ggcggtgggc ggctcgtcgt
     1621 tcgtgatcca catcgcccac gccgccaccg ccatcgccac cgggcgctgt gaggtggcgc
     1681 tggtgacgca cggcgagagc gggcgcagcc gggtcgggat gccgccccgg tcgatggatc
     1741 cgcagtcgct gcgcggccag ttcgaggcgc cctacgggct gccgcagccg gtcggcgcct
     1801 acgcgctagc ctgctcgcgc catatgtatg agtacggcac gaccaaggcg cagttggccg
     1861 agatcgcggt cgccacgcgc aagtgggcca tgctcaaccc caaggcgatg atgcgcgagc
     1921 cgatcacgat cgaggatgtg ctcaactcgc ggccgatcag ttggcccttc aacctgctcg
     1981 actgctgcct ggtcaccgat gccggcggcg cctacgtgat gacctcggtc gatcgcgccc
     2041 gcgacctgaa gcagaccccg gcggtcgtgc tcggcttcgg cgaggggcac gaccacgcca
     2101 tgatctcgca gatgccgagc tacaccagct tcgcggcgcg caactcgggc aagcgcgcct
     2161 tcgagatggc cggcgtgacg cacgacgagc tgaacctggc gatgatctac gactcgttca
     2221 cctacaccgt gctcctctca ctggaggagc tgggcttctg cgccaagggc gagggcggcc
     2281 agttcgtcag caaccagcgc acggcgccgg gcggcgagtt cccgctgaac accaacggcg
     2341 gcggcctctc ctacacgcac cccggcatgt acggcatgtt cgccgtgatc gaggcggtgc
     2401 gccaactgcg gcaggactac gccgaccagg ggatccggca ggtgccggac tgcaagatgg
     2461 cgctcgtgca cggcaccggc ggggtgctgt cgtcggccgg cacggcgatc ctggctcgcg
     2521 gctagggtga gaggctagag ggagaacgag tgatgagcga gcagaagcag tacgtcaagc
     2581 ccctgccgaa gtcggacggc accacggcgc ccttctggga gagcgtcaag gcgcacgcca
     2641 tgcagatcca gcgcgacaac aagaacggga aatatttctt ctacccgcgt ggcctgagcc
     2701 cgtacgggct gcacgatgat ttctcctggc agccggtctc ggggcgcggg gtggtgcacg
     2761 ccttctcgat cgtgcacaac aaccgcgcgc ccggcttccg tgacgagatt ccgtatgtgg
     2821 tggcgatgat cgagctggag gagggcgcgc gcatgatgag caacctcatc gatgtccagc
     2881 ccgaccccga gcacgtcaag atcggcatgc cggtcgaggt cgtctacgag gacgtgaccg
     2941 acgaggtcac gctgcccaag ttccggccgc gccaggggta ggcgggccct catccccggc
     3001 tggggttccc tcacccccgt cccctctccc ggtgcgcggg agaggggcgc tgaccagcga
     3061 caaggatgag tggcatcgcg gacggatcct tcgacaagct caggatgaca gggttaatag
     3121 c
//
LOCUS       DASUBI010000585         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1049575, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000585 DASUBI010000000
VERSION     DASUBI010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049575"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2569)
                     /locus_tag="VFU72_11345"
     CDS             complement(<1..>2569)
                     /locus_tag="VFU72_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HEU5424127.1"
                     /translation="WRLRGDIDLLRLERAPDGELTLLIADMKSSTAARIEHRLQVALY
                     HEMLAALLAEAGVPHAGIALGVLYRGSEVAPLEESDEQREQRRLAGHYFNVPESYLEL
                     IADPEVYRATVRDLVTGPGSHAERAALAEFTQIPFHLSYKCDGCLYNEFCMKWSAEHD
                     DLSLLAHLSGADKRALIQAGVVTTRQLAALKESAQGNGGRTLVPAPGQEALIKRLGTS
                     YSVGPHLDELIERARQYQRFQRHEPHQSSDLPSLGYGSLPYSDPDHNPNLVTVYIDAQ
                     HDYLHDRIYLAGSLAVARRAGKPVRRRSLVHLAPAPVADGDAERELLVAWVRDTLRAI
                     VELAEPDAQGEPNAPVHLVLFNSFGRDLLLQGLARHFETVLGLTPLYDFMTQLAAFDS
                     PVLTFLDQEIRELKNYPMVCQSLQSVATFLKFDWRTPRPFRRLFRERLFDYVGKLGGD
                     DGEWYTRRARFNSQIPLEYAYAAWHELQAPAPGERDDYGPYRQVTPELLRAFQARRLE
                     ALECIAGDFKGNKQTVKTAFRLPDLNRFTEKARNLAQALDEFVTIERHAALSAWKNAR
                     LAPPERRVLAGDTLLVRYRPEEQDVETRVRNRHNRERYRQRQRLEREYLRAHPDEERA
                     TLTKEQRGATKVEQNGLRFRLELCVEGCDCDLDEALALSTLREGERVVISPRWDHDSR
                     LPEEEQAPYTPTPKAMLYKARAEIERIVVARDDDGRAVSGYVDVEMREWNANLPGFCF
                     SSFAAEPFENGALYTLDVDPNDIYGYWCSELTKKLCQHDDNPLYQRLAGGGPAAAPWP
                     ENAAAAQARFFTGLEELQRHGAFEPFEASKRDFIAAHGEAATLLVQGPPGTGKSFGTA
                     "
BASE COUNT          349 a          876 c          930 g          414 t
ORIGIN      
        1 aggcggtgcc gaagctcttg ccggtaccgg gcgggccctg caccagcagc gtggccgcct
       61 cgccgtgggc cgcgatgaaa tcgcgcttgc tcgcctcgaa gggctcgaag gcgccgtgtc
      121 gctgaagctc ctccagcccg gtaaaaaagc gcgcctgcgc cgccgccgcg ttttcgggcc
      181 agggcgcggc ggccgggccg ccgcctgcca gccgctggta gagcgggttg tcgtcgtgct
      241 ggcagagctt cttggtgagc tccgagcacc agtagccgta gatatcgtta gggtccacgt
      301 cgagcgtgta gagcgccccg ttctcgaacg gctcggcggc gaacgaggag aagcagaagc
      361 cgggaagatt ggcgttccac tctcgcatct cgacgtcgac gtagccactg accgcccggc
      421 cgtcgtcgtc gcgtgccacc acgatgcgct cgatctcggc gcgtgctttg tagagcatcg
      481 ccttcggcgt cggcgtgtag ggggcctgct cctcctcggg caggcgcgag tcgtggtccc
      541 agcgcggcga gatcaccacc cgctcgccct cgcgcagcgt actcagcgcc agcgcctcgt
      601 ccaggtcgca gtcgcagccc tcgacgcaca gctccagccg gaagcgcagg ccgttctgct
      661 ccaccttcgt cgcgccccgc tgctctttgg tcagcgtggc cctctcctcg tccgggtgcg
      721 ctcgcaggta ctcgcgctcc agccgctgcc gttgccgata gcgctcgcgg ttgtggcggt
      781 tgcgcacgcg cgtttcgaca tcctgctctt ccggccggta gcgcaccagc agggtgtcgc
      841 cggcgagcac gcggcgctcg ggcggggcca ggcgcgcgtt cttccaggcc gagagcgccg
      901 cgtggcgctc gatggtgacg aactcgtcga gcgcctgggc caggttgcgc gccttctcgg
      961 tgaagcggtt gaggtcgggc aggcggaagg cggtcttcac ggtctgcttg ttgcccttga
     1021 agtcgccggc gatgcactcc agcgcctcca gccgccgcgc ctgaaacgcg cgcagcagct
     1081 ccggtgtgac ctggcggtag gggccgtagt cgtcgcgctc gccgggcgcg ggcgcctgca
     1141 gctcgtgcca ggcggcgtag gcgtactcca gcgggatctg gctgttgaag cgcgcgcgcc
     1201 gggtgtacca ctcgccgtcg tcgccgccca gcttgccgac gtagtcgaag aggcgctcgc
     1261 ggaagaggcg ccggaagggc ctgggggtgc gccaatcgaa tttgaggaag gtggcgaccg
     1321 actgcagcga ctggcagacc atggggtagt tcttcagctc acggatctcc tggtcgagga
     1381 aggtcagcac cggcgagtcg aaggccgcga gctgggtcat gaagtcgtag agcggggtca
     1441 ggccgagcac cgtctcgaag tggcgcgcca gcccctgcag cagaaggtcg cgcccgaagc
     1501 tgttgaacag caccaggtgc accggcgcgt tcggctcgcc ctgggcgtcc ggctcggcca
     1561 gttcgacgat cgcgcgcagc gtgtcgcgca cccaggccac cagcagctcg cgctcggcgt
     1621 cgccatccgc caccggcgcc ggcgccaggt ggacgagcga gcgccggcgc acgggcttgc
     1681 ccgcgcgacg cgccaccgcc agcgacccgg ccaggtagat gcggtcgtgc aggtagtcgt
     1741 gctgggcgtc gatgtagacg gtgacgaggt tggggttatg gtcgggatcg ctgtagggga
     1801 gcgagccgta gcccaggctc ggcaggtcgg aggattgatg cggctcgtgc cgctggaagc
     1861 gctggtactg ccgggcacgc tcgatcagct cgtccagatg cggtccgacg ctgtagctgg
     1921 tgccgagccg cttgatcaag gcctcctggc cgggcgccgg cactagcgtc cgtccgccat
     1981 tcccctgcgc cgactccttg agtgcggcga gctggcgcgt cgtgacgacc ccggcctgaa
     2041 tgagcgcgcg cttgtccgcc ccgctcaggt gcgccagcag cgagagatcg tcgtgctcgg
     2101 cgctccactt catgcagaac tcgttgtaga ggcagccgtc gcacttgtag ctcagatgga
     2161 aggggatctg ggtgaactcc gccagcgccg cgcgctcggc gtgcgagccc ggcccggtga
     2221 ccaggtcgcg cacggtggcg cggtagacct cggggtccgc gatcagctcc aggtagctct
     2281 cgggcacgtt gaagtagtgc ccggccaggc ggcgctgctc gcgctgctcg tcgctctcct
     2341 ccagaggcgc gacctcgctg ccccgataga ggacaccgag cgcgatccca gcgtgcggca
     2401 cgccggcctc ggccagcagc gccgccagca tctcgtggta gagcgcgacc tgcagccggt
     2461 gctcgatgcg cgcggcggtg gagctcttca tgtcggcgat caggagcgtg agttcgccgt
     2521 ccggcgcgcg ctccagccgc agcaggtcga tgtcgccgcg caggcgcca
//
LOCUS       DASUBI010000586         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1240466, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000586 DASUBI010000000
VERSION     DASUBI010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240466"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFU72_11350"
     CDS             complement(<1..195)
                     /locus_tag="VFU72_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424128.1"
                     /translation="MKGWIGGLGGAVVITVGVVVAGLAALAFVAFNGGGSTHTDTGKV
                     GRVDVGSHRHDRGGTRAAAGT"
     gene            complement(262..1563)
                     /locus_tag="VFU72_11355"
     CDS             complement(262..1563)
                     /locus_tag="VFU72_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5424129.1"
                     /translation="MGAPLRRLEVDHPHGVSRRPARVPQPHRRFRRTPPRGRRSYPTT
                     GRGETSWRANLYRAASVRPPRAFPTERTLLARPRRQAVSPSSPSRSRSRAAAHRPARR
                     LNSFHGYRDLVPLARLLARRPALAVTAGAVTIAFSAILVKLSHASPSTAAVFRCAYAL
                     PVLGLLAWMERRRYGPRARGDLKLSIIAGAFFAADLICWHHAIHDVGAGLATVLGNLQ
                     VVLVGVLAWVLLSERAEARVFAAVPVVFIGVLLISGVVGSGAYGLDPARGVLFGVFTG
                     LTYAGFILVLRQSGSDMRRPAGPLFEATLAATIFAIPAGAALGDLNLVPSWPGHGWLA
                     VLALSSQVLGWLLISISLPRLPAALTSVLLTIQPVGSVLLGVAIFGESPSAAQAAGVA
                     VVLAGVMVASLGPRRAPAPIVARQAAAIDAGSPASINARQG"
     gene            1606..2226
                     /locus_tag="VFU72_11360"
     CDS             1606..2226
                     /locus_tag="VFU72_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424130.1"
                     /translation="MAKQRQIKMQQRVGSLAAIQQLESRTDEQLLSESKHRAAARAIL
                     GARAAERYDAKTARQYFNEALAGCHPQERPALRQMMKASLALAERRPDELRAAVAKLG
                     QEPPSARQLLMLRLMSLVAPPPGSSILLRARGYLLLLLIIAVLLGIGFGLANLIAIPF
                     GGVSTGVGIFYGLVLVAVVLGVLVFLGRRRQKKAQAERSARIAGGG"
BASE COUNT          369 a          834 c          817 g          280 t
ORIGIN      
        1 cgtcccggcg gctgcacgtg tccccccacg gtcgtgccgg tgggagccga cgtcgactct
       61 ccccaccttg cccgtgtccg tgtgcgtgga gccgcctccg ttgaacgcga cgaaggcaag
      121 ggccgcgagc ccggccacca ccacaccgac cgtgatgacc accgcgccgc ccagccctcc
      181 gatccacccc ttcatcgcgc gcatgccggc cgattgttcc gggcgaaaga agggctgtcc
      241 aggcgttttt ggacggatgt cctagccctg gcgagcgttg atggaggcgg gtgacccagc
      301 gtcgatggct gctgcctgcc gggccacgat gggcgccggc gcgcgacgcg gccccagact
      361 cgccaccatc acgccggcca gcaccaccgc cacgcccgcc gcctgggccg ccgagggcga
      421 ctcgccgaag atcgccacac cgagcagcac cgagcccaca ggctggatcg tcaacaggac
      481 ggacgtgagc gcggcgggca gccgcggcag cgagatcgag atgagcagcc agccgagcac
      541 ctgcgagctg agcgcgagca cagcgagcca gccgtggccg ggccagctcg gaacgaggtt
      601 gagatcgccc agcgcggcac cggccgggat cgcgaagatc gtggccgcca gggtcgcctc
      661 gaagaggggc ccggccgggc gtcgcatgtc gctcccggac tggcgcagga ccaggatgaa
      721 gcccgcgtac gtgagccccg tgaagacgcc gaacagcacg ccgcgggccg ggtccagccc
      781 ataggcgccg ctgcccacga ccccggagat cagcaatacg ccgatgaaca ccaccgggac
      841 tgcggcgaac acccgcgcct ccgcacgctc gctgagcagc acccacgcca gcacccccac
      901 gagcaccacc tggagattgc cgagcactgt ggcgagcccg gcccccacgt cgtggatcgc
      961 gtggtgccag cagatgaggt cggccgcgaa gaacgcgccg gcgatgatcg acagcttgag
     1021 gtcgccgcgc gcccgcgggc cataccgccg ccgctccatc caggccagca acccaagcac
     1081 cggcagcgcg tacgcgcagc ggaataccgc agcggtggac ggcgacgcgt gcgagagctt
     1141 cacgaggatc gccgagaacg cgatggtcac cgcgcctgcg gtgacggcca gggcgggacg
     1201 gcgcgcgagg agacgggcca ggggcaccag atcacggtac ccatgaaaag agttgagccg
     1261 ccgcgcgggg cgatgcgcgg cggctcggga gcggcttctg ctcgggctcg aaggcgaaac
     1321 tgcctgccga cggggccggg cgagaagcgt gcgctcagtt gggaaggctc gcggtggccg
     1381 taccgaggcg gcccggtaca gattcgcgcg ccatgaagtc tctcctctgc ctgtggtggg
     1441 atacgaccgg cgcccccgcg gcggtgtccg acggaagcgc ctgtgcggtt gaggtacccg
     1501 cgccggacgc cgtgaaaccc catgcgggtg atcaacctcc aatcggcgta gcggcgctcc
     1561 catgcgacgg cctgcgagcc gccccgtctg ccagactgac gtgccgtggc caagcagcgc
     1621 cagatcaaga tgcagcagcg cgtgggcagc ctggccgcca tccagcagct cgagagccgc
     1681 accgacgaac agctactcag cgagtcgaag caccgcgcgg cggcccgcgc catcctcggt
     1741 gccagggccg ccgagcgcta cgacgcgaag accgctcgcc agtacttcaa cgaggccctc
     1801 gcgggctgcc atccgcagga gcgccccgcg cttcgccaga tgatgaaggc gtcgctggca
     1861 cttgccgagc ggcggccaga cgagttgcgc gcggccgtgg cgaagctggg ccaggagccg
     1921 ccaagtgccc gccagctgct gatgctgcgg ctgatgagcc tcgtggcgcc gccgccgggg
     1981 tcatcgatcc tgctgcgcgc ccgcggctac ctgctgctgc tcctcatcat cgccgtgctg
     2041 ctcgggatcg gcttcggcct cgccaacctc atcgcgatcc cgttcggcgg cgtgagcacc
     2101 ggcgtaggca tcttctacgg gctcgtgctg gtggcagtgg tgctcggcgt gctcgtgttc
     2161 ctgggacgca ggcgccagaa gaaggcgcag gccgaacgct cggcccgcat cgcgggcggc
     2221 ggctgacgcc tcagttgagc gcggccgcca gccggcggcg gtggcggctc gcgagctcgc
     2281 tctgcgggcc cagctcggtg
//
LOCUS       DASUBI010000587         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_939726, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000587 DASUBI010000000
VERSION     DASUBI010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939726"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..451)
                     /gene="mraZ"
                     /locus_tag="VFU72_11365"
     CDS             complement(23..451)
                     /gene="mraZ"
                     /locus_tag="VFU72_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU5424131.1"
                     /translation="MFLGRYSHSLDAKGRVAIPARFREEIGTEAVLTRGIDRCLSLYP
                     LSAWQPLADKVSGLSISDPDARAFRRMFFAEAANVEFDRQGRMLIPPDLRRYAGLDGE
                     ALVVGMHTYVEIWSSAAWESQAELMDREGSSIAQRLASLI"
     gene            complement(899..1756)
                     /locus_tag="VFU72_11370"
     CDS             complement(899..1756)
                     /locus_tag="VFU72_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424132.1"
                     /translation="MGDVVDAERWEPPVAAARESGRARRRGGPTDWGVALELLGGRLL
                     AALVFLVGGLSRAPAFWLPLAGCALVVGLILMGCFYHGRAGAAAQLVWLADAVTLGVI
                     LPLLLFNAFAATGAAPLQASEAAVYLETAAAALLLLAGLFLAVSWRAGADRLLAGVAL
                     LPAALLAPGLAEAHRDLRDATVAGMLALAWLLAALVLLAARWLGGGPGRLLPSLAYLG
                     FILVLLVSDASFATFGSQGAPVGLVEPLLLLLCGLGLLAVAVGGQRRTRPRRTRRGGR
                     PAAYQENEE"
     gene            1849..>2153
                     /locus_tag="VFU72_11375"
     CDS             1849..>2153
                     /locus_tag="VFU72_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5424133.1"
                     /translation="MAGETTRLGIIGGTFDPIHLGHLIIAEEFWYRLRLERVLFLPTS
                     SPPHKVGQAISPVDDRLAMLRLAIDGNPHFAISVIDVERPGLTYTADTLPILQARNPR
                     "
BASE COUNT          360 a          801 c          715 g          277 t
ORIGIN      
        1 tgatcgagca tgaagcgatt ccctagatga gcgacgcgag acgttgggcg atgctggagc
       61 cttcgcggtc catcagctcc gcttgtgact cccaggcggc cgaactccag atctcgacgt
      121 aggtatgcat ccccaccacc agggcctcgc cgtcgagacc ggcatagcgt cgcagatcag
      181 gcgggatcag catgcgtcct tggcggtcga actccacgtt ggcggcttcg gcgaagaaca
      241 tgcggcgaaa ggcgcgcgcg tccggatcgc tgatcgagag gccggagacc ttgtcggcca
      301 gcggctgcca ggcgctcagt gggtagagcg acaggcagcg atcgatgccg cgcgtcagca
      361 cggcttcggt gccgatctcc tcgcggaagc gcgccgggat ggcgacgcgc cccttggcgt
      421 ccaggctatg gctgtatcgc ccgaggaaca cggagtagcg cccccaatag ccttcccatg
      481 ccgcccactg cgggcggcgg ccgcaacgat tcggcgttca acgaccggtg gaatcgctca
      541 gtgccgcggg atcttgtcgt gcgcgccgga gtcgtgtccc cgcgagcccc accgcgccgg
      601 ccggttgtcc acatcggccc ccacttatcc ccactgagcc ccaccgttgt ccacattata
      661 ccgtcgcagg gctccctggc aaccgaattt atgtcgaata gggctcgttc gtacacgatg
      721 ttcgttcgaa ttggctatgt ggcgccgcga gacggggaag tgccagtgag gacgcatcct
      781 tcgacaggca caggatgaca agggggagcg gatgcggcct ctcggacgaa ggcagtcctc
      841 ctcgttcgcc cctcctgaac cccgtcatcc cgaccgaagt ggagggatcc gtcttgccct
      901 actcctcgtt ctcctggtac gcggccggcc ttcccccgcg ccgcgtccgg cgcgggcgtg
      961 tccgtcgctg cccacccacg gcgaccgcca gcaagcccag gccgcagagc agcagcagca
     1021 gcggctcgac cagccccacc ggcgcgccct ggctaccgaa cgtggcgaag ctcgcatcac
     1081 taaccagcag gaccaggatg aagcccaggt aggccaacga gggcagcagc cgcccgggcc
     1141 cgccacccag ccagcgcgcc gccagcaaga ccagcgccgc cagcagccag gccagcgcca
     1201 gcatgccggc gaccgtcgcg tcgcgcaggt cgcggtgcgc ctcggccagc cccggggcca
     1261 gcagcgctgc cggcaggagc gcgacccccg ccagcagccg gtccgcgccg gcgcgccagg
     1321 agacggccag gaagagcccg gccagcagca gcagcgcggc tgccgccgtc tccagataga
     1381 ccgccgcctc ggacgcctgc agcggcgccg cgccggtggc ggcaaaggcg ttgaagagga
     1441 ggagtggcag gatcacgccc agggtgacgg catcggccag ccagacaagc tgcgcggcgg
     1501 cgcccgcccg gccgtggtag aagcagccca tcaggatcag cccgacaacc agcgcgcagc
     1561 ccgccagcgg cagccagaag gccggcgcgc ggctcagccc gccgaccagg aagaccagcg
     1621 ccgccagcag ccgcccgccc agcagctcca gcgccacccc ccagtcggtc ggcccgcccc
     1681 gccggcgcgc tcggccagat tcgcgcgctg ccgcgaccgg cggctcccag cgctcggcgt
     1741 ccaccacgtc ccccatagtg ccccccacga tcggttcagc cgcgaagtct agcaccgggg
     1801 caatggtagg atgggccggg ttggcgcagg cgagcgagag cgggtggcgt ggcgggcgag
     1861 acgacgcggc tggggatcat cggcggcacc tttgatccga tccatctggg gcatctgatc
     1921 atcgccgagg agttctggta ccggctgcgg ctggagcgcg tcctcttcct gccgaccagc
     1981 agcccgccgc acaaggtcgg ccaggcgatc agcccagtgg acgatcgcct ggcgatgctg
     2041 cgcctggcca tcgacggcaa cccccacttc gccatcagcg tgatcgacgt cgagcgcccc
     2101 ggcttgacct acaccgccga cacgctcccg atcctgcagg cccgcaaccc ccg
//
LOCUS       DASUBI010000588         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_617756, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000588 DASUBI010000000
VERSION     DASUBI010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617756"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFU72_11380"
     CDS             complement(<1..217)
                     /locus_tag="VFU72_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEU5424134.1"
                     /translation="MFRRLAVIAVIIASYFALSRFVDLESLAAGVFSLPSNVLLQLLV
                     LLFCAELVKGVRWAYFLRASELDIRPLD"
     gene            433..1392
                     /locus_tag="VFU72_11385"
     CDS             433..1392
                     /locus_tag="VFU72_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424135.1"
                     /translation="MTAVPLGDEVAPPIELRQLTKRYGTRRGVLDLDLTVAEGEIFGF
                     LGPNGAGKTTTIRVLMGLIRPSAGEARVFGRDCWRQSTEVKALVGFLPGDVHLQEQLS
                     GCDVLAFFAAFRGSGDLTRGLKLAADLDLDLDVRVKHLSKGNRQKLAVVQALMHDAPL
                     IILDEPSSGLDPLMQATLLQLLREEQSRGRTVFLSSHVLPEVEQVAERVAIIREGRLV
                     AVEDVARLKALREREMLVLLAEPVPAAPERLAALPGVRVLSVDPAGRQLRLAVRGAPG
                     PLLRLLSELPVEDLTAGPPDLEEVFLHYYTGDGKTPDVAAADR"
     gene            1389..2192
                     /locus_tag="VFU72_11390"
     CDS             1389..2192
                     /locus_tag="VFU72_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5424136.1"
                     /translation="MSSVMFRRALWGLRWTVFWYALGIALYVAIVLSAYPTVRGNAAD
                     IQSLLESYPDTLLRALGASRDLASLPSYLGVESLNLIWPLIVAIFVIMSGAAVVAQEV
                     ERGTADLWLSVPESRARLLLGKLVALLVGVVVLALATDVMLGLGALLVGSALGLGQLA
                     ALATLLIAFPLAIAGYAALFSALMSERSKAAALAAALTLGFYLIWVVAGLIARLHWLG
                     YLSIFTAYDPTPTLAGGDVPWLHVLVLVLVGLLCAGGALVVFTRRDIAT"
     gene            complement(2206..>3580)
                     /locus_tag="VFU72_11395"
     CDS             complement(2206..>3580)
                     /locus_tag="VFU72_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU5424137.1"
                     /translation="VIRAKDDKRDLDAPDHERRDADALSGEGALGRAEDRTVESARAV
                     LTGATRANWFQRLLPFLGPAFIASVAYVDPGNFATNIQGGSEFGYLLLWVIVASNLMA
                     MLIQALSAKLGIATGRNLAELCRDRFPRSVVWAMWGLAELVAMATDLAEFLGAALGFN
                     LLFHIPLFPAAILTGIATFAILALERRGFRSLEAVIAVLVGVIAVSYLIETLLDQPDW
                     AAIGFHAVVPQFSGQESILLAVGILGATVMPHVIYLHSSLTQNRIVPSNPQQARRIFR
                     FELIDVVIAMGIAGAVNAAMLIMAAATFHASGNTHVASIEDAHLTLKPLLGSLSSTIF
                     AVSLLASGLSSSSVGTMAGQVIMQGFLHRQIPVWLRRLITMLPTFVVIGIGLDPTRTL
                     VISQVVLSFGIPFALVPLVLFTKNRQLMGELVNRRRTNVAITAIAGLIVALNLYLLYS
                     TVGGWLA"
BASE COUNT          573 a         1152 c         1288 g          567 t
ORIGIN      
        1 cgtcgagcgg gcgaatgtct agctcgctgg cgcgcagaaa ataggcccag cggacccctt
       61 tgaccagctc agcacagaag aggagcacca ggagctgcag cagaacattg gagggcaggc
      121 tgaagacccc tgccgccagc gactcgaggt cgacaaacct ggagagcgca aaataggacg
      181 caataatgac cgcgatgacc gcgagtcgtc gaaacaacag aacacgaccg atctgacgag
      241 ggttaaggtc gggcaccaag ccgacagctt gccgcataat tcggaaattc gatgccactg
      301 ttcgcgtcgc gcggccccaa ccgcaccctg atcgcgccca cgggcgacct tgtctcgtct
      361 gaactcaact gtaccccatc gcaccgccgc cggccatacg ccttttgtct acaataggtg
      421 cggaaacggt cgatgaccgc cgtaccgctg ggggatgagg tggcgccacc gatcgaattg
      481 cgacaactga ccaagcgcta cggcacgcgc cgcggcgtgc tcgatctcga cctgaccgtg
      541 gccgagggag agatcttcgg cttcctcggt cccaacggcg ccggcaagac gacgaccatc
      601 cgcgtgctga tggggctgat ccgccctagc gcgggcgagg cgcgtgtctt cgggcgcgac
      661 tgctggcgcc agtccaccga ggtcaaagcg ctcgtcggct tcctgcccgg cgatgtgcac
      721 ctgcaggaac agttgagtgg gtgcgacgta ctcgccttct ttgccgcctt ccgcggcagc
      781 ggcgacctga cacgcggcct caagctggcc gccgacctcg acctcgatct tgatgtgcgc
      841 gtcaagcacc tctcgaaggg gaatcgccag aagctggccg tcgtgcaggc gctgatgcac
      901 gacgccccgc tgatcatcct ggacgagccg agcagcggcc tcgatcctct gatgcaagcg
      961 acgctgctgc agttgctgcg cgaggagcaa tcgcgcggcc gcaccgtgtt cctttcctcg
     1021 cacgtgctgc cggaggtgga gcaggtcgcc gagcgggtgg cgatcattcg cgaggggcgc
     1081 ctcgtggcgg tcgaagacgt ggcgcggctc aaggcgttgc gcgagcggga gatgctggtg
     1141 ctgctggcag agccggtgcc cgccgcgccg gagcggctgg cggcgctgcc gggcgtgcgc
     1201 gtgctcagcg tcgatccggc gggccggcag ctccggctgg cggtgcgcgg ggcgccgggg
     1261 ccgctgctgc ggctgctgag cgagctgccg gtcgaggatc tgaccgccgg tccgcccgac
     1321 ctggaagagg tttttctcca ctactacacc ggcgatggga aaacgccgga tgtagcggcg
     1381 gcggaccgat gagcagcgtg atgttccgcc gggcgctctg ggggctgcgc tggacggtct
     1441 tctggtacgc gctggggatc gcgctctacg tcgcgatcgt cctgtcggcc tacccgaccg
     1501 tgcgcggcaa cgccgccgac atccaatcgc tgctcgagtc gtaccccgat acgctgctgc
     1561 gtgctctggg cgcctcgcgt gatctggcca gcctgccgag ctacctcggc gtcgagtcgc
     1621 tgaacctgat ctggccgctg atcgtggcga tcttcgtgat catgagcggc gcggccgtgg
     1681 tggcgcagga ggtcgagcgc ggcacggccg atctctggct ctcggtgccg gagagccgcg
     1741 cccggctact gctgggcaag ctcgtcgcgc tgctggtggg cgtggtggtg ctggcgctgg
     1801 cgaccgatgt catgctgggg ctgggcgcgc tgctggttgg ctccgcgctc gggctcggcc
     1861 agctcgccgc gctggccacg ctgctgatcg cctttcccct ggcgatcgcc ggctacgccg
     1921 cgctcttctc ggcgctgatg agcgagcgca gcaaggcggc cgcgttggcc gcggcgctga
     1981 cccttggctt ctatctgatc tgggtcgtcg ccggtctgat cgcgcggctc cactggctcg
     2041 gctatctctc gatcttcacc gcctacgacc caacgcccac gctagccggc ggcgacgtcc
     2101 cctggctcca cgtgctggtg ctcgtgctgg tcgggctgct ctgcgcgggc ggggcgctcg
     2161 tcgtcttcac ccgccgcgac atcgcgacct agcgcggccg cacggtcagg ccagccagcc
     2221 gccgaccgtg ctgtagagca ggtagagatt cagcgccacg atcagcccgg cgatcgcggt
     2281 gatggcgacg ttggtgcggc gccggttgac cagctcgccc atcagttggc ggttcttggt
     2341 gaagaggacc agcgggacga gcgcgaaggg gatgccgaag ctcaacacca cctggctgat
     2401 gaccagggtg cgcgtcgggt cgagcccgat gccgatcacc acgaaggtgg gcagcatcgt
     2461 gatcaggcgg cgcagccaga ccgggatctg gcggtgcagg aagccctgca tgatgacctg
     2521 gccggccatc gtgccgaccg aggatgacga gagtcccgag gccagcagcg agaccgcgaa
     2581 gatggtgctg gagagcgagc cgagcagcgg cttcagggtc aggtgcgcgt cctcgatcga
     2641 ggccacgtgg gtgttgccgg aggcgtggaa ggtcgcggcc gccatgatca gcatggccgc
     2701 gttgaccgcg ccggcgatcc ccatcgcgat gaccacgtcg atcagctcga agcggaagat
     2761 gcggcgcgcc tgctggggat tgctgggtac gatgcggttc tgggtcagcg aggagtgcag
     2821 gtagatgacg tgcggcatca cggtcgcgcc gaggatgccg accgccagca ggatgctctc
     2881 ctgcccggag aactgcggca ccacggcgtg gaagccgatg gcggcccagt cgggctggtc
     2941 gagcagcgtc tcgatcaggt agctgacggc gatgacgccg acgaggaccg cgatcacggc
     3001 ttccagcgag cggaagccgc ggcgctccag cgcgaggatc gcgaaggtgg cgatgccggt
     3061 caggatggcg gcggggaaga gtgggatgtg aaagagcagg ttgaagccga gcgccgcgcc
     3121 gagaaactcg gccaggtcgg tggccatggc caccagctcg gccaatcccc acatagccca
     3181 gacgacggat cgcggaaagc ggtcgcggca gagctcggcc agattgcgcc cggtggcgat
     3241 accgagcttg gccgagagcg cctggatcag catggccatc aggttactgg cgacgatcac
     3301 ccagagcagc aggtagccga actcggagcc gccctggata ttggtggcga agttgccggg
     3361 gtcgacgtag gcgacgctgg cgatgaaggc cggaccgagg aagggcagca ggcgctggaa
     3421 ccagttggct cgcgtcgcgc cggtcaggac ggcgcgggcc gactcgaccg tgcgatcttc
     3481 ggctcggccg agggctccct cgccgctcag cgcgtcagca tcccggcgct cgtggtcggg
     3541 ggcgtccagg tcacgcttgt cgtccttcgc acgaatcacg
//
LOCUS       DASUBI010000589         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_411991, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000589 DASUBI010000000
VERSION     DASUBI010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411991"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..488
                     /locus_tag="VFU72_11400"
     CDS             201..488
                     /locus_tag="VFU72_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424138.1"
                     /translation="MYVRILTGRVKPYNADLFGRVLTDDYVPLIRQLPGFQHFYFGIE
                     RSTWELVAVMLWDRVPPDDQIMEIGEPFLDQLRALGAELQPWHEYEILTSG"
     gene            complement(534..1589)
                     /locus_tag="VFU72_11405"
     CDS             complement(534..1589)
                     /locus_tag="VFU72_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEU5424139.1"
                     /translation="MFDYQQQPFVLAWELTRACNLACVHCRAEAQLRRDPRELTSDEA
                     MRLVDEIASFDPAPTLILTGGDPMRRPDLTELIQHATFRGLRTALTPAGTALASRARL
                     EAAKQAGLARVAVSLDGSTAERHDAFRRVAGSFGWTLAIARHAAEIGLPLQVHSTLSR
                     QTVDDLPALADLADELGAVVWAVFCLVPTGRAQFADELDADEYERQFHWLAARAHSAN
                     WKLKLTEGYHFRRLMLQQAERPRPTSAVDGIGRAPLPVNAGNGFCFVSHIGDVCPSGF
                     LPLVAGNVRQDSLVELYQRHPLFRALRDPALLRGKCGGCEFRAVCGGSRSRAFAHTGD
                     YLASDPACAYQPLADHR"
     gene            complement(1639..>2535)
                     /locus_tag="VFU72_11410"
     CDS             complement(1639..>2535)
                     /locus_tag="VFU72_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU5424140.1"
                     /translation="LIVLGGVVRVTGAGLGCGNDWPVCNGQVVPHFTLLTALEYAHRV
                     AAATVTLLTAALVVAGWRLRRHDRRLLAIPALALVLVIAQAGLGAVTVFLDLPALVVT
                     AHLGLAESYLATLIVLAGVAFSRPAAPLARRPAPPAISALVAGAAVALVLLSGAYTAA
                     TNASYACWRWPTCGGGRLVPTGWTPADIQLLHRWLVIVAGLAIGALLIRVRHAPRVSR
                     RAVVLASAAGTLFVAEVLLGALVIWLHFPAGVRAAHLGVATLIWGLIVALVVNEQVSQ
                     SGEERRAAGTVDRALDARVGAS"
BASE COUNT          407 a          875 c          907 g          346 t
ORIGIN      
        1 gctcgcggga tcggccccga gcgccggtca aatacaggta tgggacggcg aggcgcctct
       61 ggcttgacgg tcgctgggcg ttacgtggcc gttgctcgag ccccggcgcc aagcgtggcc
      121 acgcgccgtc gcaccgcggc cacgcgtttg cgggtccggg ccgcgacggc gtcgtacgag
      181 ccgaagcgga gtgtatctcg atgtacgtgc gcattctcac cgggcgtgtc aagccctaca
      241 acgcggatct gttcggtcgg gtgctaaccg acgactatgt cccgctcatc cggcaactgc
      301 ccggctttca gcatttttac ttcggcatcg agcgcagcac ctgggagctc gtcgcggtga
      361 tgctctggga tcgcgtgccg cctgatgacc agatcatgga gatcggcgaa ccctttctgg
      421 accaactacg tgcgttgggc gcggagttgc agccctggca cgagtacgag attctgacct
      481 ccggctgagc cggtaaccgc cctcgcggca cctgaatcgg ggagaaaacc gcgtcagcgg
      541 tgatcggcca ggggttgata ggcgcaggcg ggatccgatg ccaggtaatc cccggtgtgg
      601 gcgaaggcgc gcgagcgcga gccgccgcag actgcccgaa actcgcagcc gccgcacttt
      661 cccctcagca gcgcgggatc acgcagcgcg cggaagagcg ggtgccgctg gtagagctcg
      721 accagcgagt cctggcggac gttgccggcc accagcggca ggaacccgct ggggcagacg
      781 tcgccgatgt gcgagacgaa gcagaagcca ttgccggcgt tgaccgggag cggcgcgcgg
      841 ccgatgccgt ccacggcact ggtcgggcgc ggccgctcgg cctgctgcag catcagccgc
      901 cggaagtgat agccctcggt caacttgagc ttccagttag cgctgtgcgc gcgcgcggcc
      961 agccagtgga actggcgctc gtattcgtcc gcgtccagtt catcggcgaa ttgggcgcga
     1021 ccggtcggca ccaggcagaa gactgcccaa acgacggcgc cgagctcgtc ggcgaggtcg
     1081 gccagcgccg gtagatcgtc gaccgtctgc cgcgagagcg tgctgtggac ctgcagcggc
     1141 aggccgatct cggcggcgtg gcgggcgatc gcgagagtcc agccgaacga tccggccacc
     1201 cggcggaagg cgtcgtgccg ctcggccgtc gagccgtcga ggctaaccgc cacgcgcgcc
     1261 agccctgcct gcttggctgc ttcgagacgc gcccggctgg ccagcgccgt accggccggc
     1321 gtgagcgcgg tacgtaggcc gcggaaggtc gcgtgctgga tcagctcggt caggtccgga
     1381 cggcgcatgg gatctccgcc agtcaggatc agggtgggcg ctggatcgaa gctcgcgatc
     1441 tcgtccacca gccgcatcgc ctcgtcgctg gtcaattccc gtgggtcgcg gcgcagctgc
     1501 gcctcggccc ggcaatgaac gcaggccaga ttgcaggcgc gcgttagctc ccaggccagc
     1561 acgaacggct gttgctgata gtcgaacacg ggcgctgagc gttgaatcga cgcgctcaca
     1621 tccagcctcc gaatttctct acgatgcccc cacgcgcgcg tcgagcgcgc ggtcgacggt
     1681 gccggcggcg cggcgttcct ccccgctctg gctcacttgc tcgttcacca ccagcgccac
     1741 gatcaatccc cagatcaaag tagcgactcc gaggtgggcg gcgcgaacgc ccgccgggaa
     1801 gtgcagccag atcaccagcg ctccaagcag gacctcggcg acgaagaggg tgccggcggc
     1861 gctggccagc acgacagcgc gtcgtgacac gcgcggcgca tgccgcaccc ggatgagcaa
     1921 ggcgccgatg gccaggccgg ccacgatgac cagccagcgg tggagcagtt ggatgtcggc
     1981 cggcgtccag ccggtcggca cgagcctgcc gccaccgcag gtcggccagc gccagcaggc
     2041 gtacgaggcg ttggtcgccg cggtgtaggc tccgctcaac aacaccagcg cgaccgcggc
     2101 ccccgccacc agcgccgaga tggccggcgg ggcgggccgg cgggccagcg gggcggccgg
     2161 ccggctgaag gcgactcccg cgagtacgat cagcgtcgcc aggtagctct cagccaggcc
     2221 gaggtgtgcg gtcacgacca gcgcgggcag gtcgagaaag accgtcacgg cccccagccc
     2281 tgcctgcgcg atcaccaaca ccagcgccag ggcgggaata gccagcaggc ggcggtcgtg
     2341 gcgtcgcagc cgccagccgg cgacgaccag cgcggccgtc agcagggtca cggtcgcggc
     2401 ggccacgcgg tgcgcgtact ccagcgcggt caggagggtg aagtgcggca ccacctgccc
     2461 gttgcagacc ggccagtcat tgccgcagcc gagcccggcg ccggtcacgc gcacgacgcc
     2521 gccgagcacg atcag
//
LOCUS       DASUBI010000590         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1174093, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000590 DASUBI010000000
VERSION     DASUBI010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174093"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFU72_11415"
     CDS             complement(<1..1280)
                     /locus_tag="VFU72_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424141.1"
                     /translation="MERLRQTPNTLPSPEDLAALRGWSGWGPLAPALDQSRSGTWAQI
                     GERVAYLLAPEHYDHGIQATYNAFYTPPEIAAACWNILTGLGFSGGRILEPGCGAGVF
                     MAHTPPDLTVAWTGVERDPTTAAIAKLLHPGATIHAQRLEETTLPSHSVDAVLGNVPF
                     ADTKVYDPTTPRELTSNLHNYFIWRAIQALRPGGVAVLITSRYTLDASGDAATSARLL
                     ISREAELLGAIRLPSGALTSGGTEALVDVLVLRRRRADEPRRADHDTWMKTAASVGGQ
                     HVNEYLLANPQLVLGELAEDSAPRYGRTLRVNSRPDDPPVETAFAAASRPIIEQAVET
                     GRIWRVDAGASPITAESTPFKLRADGKKEGSFHLVDGAVHEVIDAQLIPVERPGKELP
                     KLIALRDAVLELLDAEADHSRPDAELAPLRAEANR"
     gene            complement(1419..1871)
                     /locus_tag="VFU72_11420"
     CDS             complement(1419..1871)
                     /locus_tag="VFU72_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424142.1"
                     /translation="MTAKSPHRDRADHRPYIETVRVALLLKDIQVGAATFDTGRVRSA
                     AMTLLAPDDDSDEPWHPTFTSAHFVELRWNEQDGWSLLALHAADAALPPTVWHRGFGV
                     LLPPEEISAWLAVLLTMPALSSSQEDGPYRSAQQHDPAFEAALAAYAS"
     gene            complement(1906..2757)
                     /locus_tag="VFU72_11425"
     CDS             complement(1906..2757)
                     /locus_tag="VFU72_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424143.1"
                     /translation="MSIDDRHTAPPTIGPSAGRRVRGRPGHTASRLTRRPRVGPQIAE
                     SGQVGNDATGMSASLTEWYAMTEPEYVLAWEQLRTWVSWLVGRYRLTLEDRLPDCWPQ
                     HPELIEELWALRAWRHEAYGPDGSGQSAVYWHQALVTFLTHVSSWWASGCRAGHASPR
                     TDIDSEHARTWAGADPLTGIPEVMRPTRSVLTNPAIEPGVITMPDINVLTSAQMQALT
                     DAGLAHPQHQAIAAFTYYDGSWWAYASKDHDPNADQDDGGAVWFRADDVDLASDHTFY
                     SQPIGTA"
     gene            complement(2754..>3735)
                     /locus_tag="VFU72_11430"
     CDS             complement(2754..>3735)
                     /locus_tag="VFU72_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEU5424144.1"
                     /translation="GANATNLLTLWMHAAALTDRSMRDVLSWSLNERDDTPVKLLRDH
                     PGAEPGSAQLLDGLYRTPPETRSGLWTTVQTALAPLLSPTAQATFCPDPGDGFDLEDF
                     LRSRGTVYLLVAEKQATALAPLIAAFVDELIETAKRLADALPGGRLDPPLGLFLDEIA
                     NVVPLPSLPALMSFAGGSGIFIAAILQSRAQAEARWGAEQAAMLWGAATVKLVLGGLT
                     GAELRDLSELVGDYDRHMTSYQRSDDGTITTGSSIQQHKTMTAEDIRTLSSAKREALV
                     IHATTPAVKIRMIRHYEGPDAALHAQAEQDARAILTEFAWSIPPAKEPVA"
BASE COUNT          538 a         1091 c         1449 g          657 t
ORIGIN      
        1 cggtttgcct cggcacgcag tggtgcgagt tccgcgtcgg ggcgggagtg gtcggcttcc
       61 gcgtcaagca actcgagcac ggcgtcgcgc agcgcgatca acttcggcaa ctccttgcca
      121 gggcgctcga ccgggatcaa ctgcgcgtcg atgacctcgt gtacggcgcc gtcgaccagg
      181 tgaaacgagc cttccttctt gccgtcggcc cgaagcttga atggcgtgct ttccgcggtg
      241 atcggcgacg cgcccgcgtc tacgcgccat atgcgtcccg tctctacggc ctgctcgatg
      301 atcggtcgac tcgcggcggc gaaggctgtc tcaaccggcg gatcatcggg gcgggagttc
      361 acgcgcaggg tgcgcccgta gcgtggcgcg ctgtcctcgg ccagctctcc gagcaccaac
      421 tgcgggttgg ccagcagata ctcgttaacg tgctggccgc cgacggaagc cgcggtcttc
      481 atccacgtgt catgatcggc gcgacgtggt tcgtctgcgc ggcgacggcg cagaacgagg
      541 acatcgacga gtgcttcggt gccgcccgat gtcagggcgc cgctgggcag gcggatcgcg
      601 ccgagtagct cggcctcgcg tgagatcagc aggcgcgcgc tcgtcgcggc gtctcccgag
      661 gcgtccagcg tgtagcgcga ggtgatcagg acagctactc cgccggggcg aagagcttgg
      721 atcgcgcgcc agatgaagta gttgtgcagg ttactggtta gctctcgcgg cgtggtgggg
      781 tcgtagactt tggtgtctgc gaaggggacg ttgccgagca cggcgtccac gctgtgcgat
      841 ggcagtgtgg tctcttcgag tcgttgcgcg tggatggtcg cgccggggtg aagcagcttg
      901 gcgatcgcgg cggtggtcgg atcgcgttcg acacctgtcc acgcgactgt caggtcgggc
      961 ggtgtgtggg ccatgaacac cccggcgccg cagccaggct ccaggatccg gccaccgctg
     1021 aagccgaggc cggtcagaat gttccagcag gctgcagcga tctcgggcgg ggtgtagaag
     1081 gcgttgtagg tggcctggat gccgtgatcg tagtgctctg gggcgagaag gtaggcgacg
     1141 cgctcgccga tctgcgccca ggtgcccgaa cgggattgat ccagagccgg ggccagcggc
     1201 ccccaacccg accagccacg cagcgctgcc agatcttcgg gcgaaggtag tgtattcggg
     1261 gtctgacgca gccgttccat ggtctccaac gcggccaggc tggcgtgtgc gcggcggact
     1321 ggaccgacgt tccctgtctc ggtggcgggg acttcggcca tggttcggcc tccaggtggt
     1381 gcgagcccgg cgcgtgccgg ggttgtgcgg ggtgggtctc aggaggcgta ggcggccagg
     1441 gctgcctcga atgcgggatc gtgttgctga gcggagcggt aggggccgtc ctcttggctg
     1501 ctggagagtg cgggcatggt cagcagtacg gccagccagg cgctgatctc ttcgggtggc
     1561 agcaggacgc cgaagccgcg gtgccagacc gtcggcggta gcgctgcgtc ggcggcgtgc
     1621 agggcgagca gtgaccagcc gtcctgttcg ttccagcgca gttcgacgaa gtgtgcgctg
     1681 gtgaaggtag ggtgccaggg ttcgtcggag tcgtcgtccg gcgcgagcag cgtcatggcg
     1741 gccgagcgca cgcgaccggt gtcgaacgtg gcggcgccga cctggatgtc tttgagcagg
     1801 agcgcgacgc ggacggtctc gatgtagggc cggtggtcgg cgcggtcgcg atgtggcgat
     1861 ttggcagtca tgtcgttctc cgggtcgcgg tgcggatcgc agtgctcagg cggttccgat
     1921 gggttgcgag tagaaggtgt ggtcgctcgc gaggtcgacg tcgtctgcgc ggaaccacac
     1981 ggcgccgccg tcgtcttgat cggcgttcgg gtcgtggtcc ttggacgcgt aggcccacca
     2041 tgagccgtcg tagtaggtga aggcggcgat ggcctggtgc tgggggtgag ccaggccggc
     2101 gtcggtgagg gcctgcatct gcgccgaggt gaggacgttg atatcgggca tggtgatgac
     2161 tcctggttcg atagcaggat tggtgaggac ggatcgggtc ggccgcatga cctcggggat
     2221 tccagtgagg ggatcggcgc ccgcccacgt gcgtgcgtgt tcggaatcga tgtccgtgcg
     2281 cgggctggcg tgtccggcgc ggcagccgct tgcccaccag gacgagacgt gcgtgaggaa
     2341 ggtgacgagt gcttggtgcc agtagacggc ggactgaccg gagccgtccg ggccgtaggc
     2401 ttcgtggcgc caggcgcgca gcgcccacag ctcctcgatc agttccgggt gctgcggcca
     2461 gcagtcagga agccggtcct cgagggtgag ccggtagcgg ccgaccagcc aggaaaccca
     2521 cgttcgtagc tgctcccacg ccaagacata ttcgggttcg gtcatcgcgt accactcggt
     2581 caacgaggcg gacatgccgg tagcgtcgtt cccgacctgg cccgactccg cgatttgtgg
     2641 accgacgcga gggcggcggg tgagccggct ggcggtgtgc cccgggcggc cgcgcacccg
     2701 acgtccggcg ctcgggccga tggtgggcgg cgcggtgtgg cgatcgtcga tgctcatgca
     2761 accggctcct tcgctggggg aatcgaccaa gcgaattcgg tgaggatggc gcgtgcgtcc
     2821 tgctcggctt gggcgtgcag cgcggcgtcg ggaccttcgt agtggcggat catgcggatc
     2881 ttcacagccg gcgtggtggc gtggatgacc agggcctcgc gcttggcgga gctcagggtg
     2941 cggatgtcct cggcggtcat cgtcttgtgc tgctgaatgg agctgccggt cgtgatcgtg
     3001 ccgtcgtccg agcgttggta gctggtcatg tggcggtcat agtcgccgac cagctcggat
     3061 aggtcgcgca attcggcgcc ggtcaggccg ccgaggacga gcttgacggt ggccgctccc
     3121 cagagcatcg cggcctgctc ggccccccag cgagcctccg cctgcgcgcg cgattgcagg
     3181 atcgccgcga tgaagatccc tgatcctcca gcgaaggaca tcagtgccgg aagggagggg
     3241 aggggaacga cgttcgcgat ctcgtcgagg aacaggccga gtggtgggtc gagtcgaccg
     3301 ccgggaaggg cgtcggcaag gcgtttggct gtctcgatca actcgtcgac gaaagcggcg
     3361 atcagcggcg cgagggccgt ggcctgcttt tcggcgacga gaagatagac cgtgccgcgt
     3421 gagcgcagga agtcttccag gtcgaagccg tcgccagggt cggggcagaa agtggcctgc
     3481 gcggtagggg agagcagcgg agccagcgcc gtctggaccg tggtccacag gccggacctc
     3541 gtctccggcg gcgtgcggta aagaccgtcg aggagttgag cgctgccggg ttcggcgccg
     3601 ggatggtcgc gcagaagctt gaccggagtg tcgtcgcgct cgttaagcga ccagctgagc
     3661 acgtcgcgca tggagcggtc ggtgagggcg gcagcatgca tccagagagt gagcagattg
     3721 gtggcgttgg cgccg
//
LOCUS       DASUBI010000591         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_823240, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000591 DASUBI010000000
VERSION     DASUBI010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823240"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFU72_11435"
     CDS             <1..200
                     /locus_tag="VFU72_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424145.1"
                     /translation="PAALKHQLGATLHADDLAAARAALLALADLVEHDMAAFTDSRAQ
                     LSPEQRAVLAGVRSRLSGINP"
     gene            346..1416
                     /locus_tag="VFU72_11440"
     CDS             346..1416
                     /locus_tag="VFU72_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424146.1"
                     /translation="MFDDLVLNVPLLRRRVPNLTAAARSVGLRPATVSNLCTGKIPIG
                     RAEVHTLVALATLAGCSLDELVLRGGLGGQIETGVKVLDLAAPLVRGGTVGLVGRAGL
                     GQLVLIMELLYRLRTRPDFATILWLPAEPLPAADEVLPQASATGTTLDEVYGLVEASR
                     AERDVLLAADRTTVLSGELLALRTQLQEPGSRPITVALFDLRGEASIEEAPYGPLDTR
                     WVFDPDLAARRLYPAVDPVSSTSVLLEGAQLEASHLTLAARTHALLRRYRELRPLVPV
                     HGLERFPPAEQLTFQRGEHLEAFLSQPLFAAEAHTKRPGRWVSLAETLADVRRILDGG
                     ADTLPVDALHDIGGLVEAEGQS"
     gene            1448..2710
                     /locus_tag="VFU72_11445"
     CDS             1448..2710
                     /locus_tag="VFU72_11445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424147.1"
                     /translation="MGTARAGEERRHLPLYALLTGTGVSIVGSLMTLIAVPWFVLQTT
                     GSPAKTGITTAVWVLPVILSGALAGPIVDRVGHKRIGVVGDLGAGLSVAAVPTLYHTV
                     GLPFAALLLIVFVRGLADFPGRTGRRSLVPQLAELAGWPLERANAAFATIQNLALLLG
                     PALAGVLIARFSPSNVMWLDAATYGFSAAMTALFVPRPAPERTATDPAERYRERLLDG
                     YRFVRRDRLLLTLLGFTTLNTLLTSPLFDVELPVYARQVFDSAVALGLMSAGYGAGAL
                     ASSFLYGAFGERLPRRAMLLGSVVLTALPLGLLTLEPPLPLAMLALGLSGFAAGPVGP
                     LYQTVVQERVGSAMRGRIFGLSGVTFVAMPLGVLAAGFLIQGFGVRLALVLQAACILA
                     AALILSRLPALHALDHAAEADEIAVRQL"
     gene            complement(2872..3366)
                     /locus_tag="VFU72_11450"
     CDS             complement(2872..3366)
                     /locus_tag="VFU72_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5424148.1"
                     /translation="MFTTRRRRWIAGAAAMLALVALITTGFTVTAKGSTYQPQTRDFT
                     VVAVPTLVHEMQGTLDYLQKDFAPGGLLDGKEVYAFSPSTLVVYQGDSVNLTLVNPAD
                     DAHTFTISDLNVNAQMPGQSSSHASFVAGKAGIYTFVCAEDEHSPYMWGQLIVLPDSQ
                     APKS"
     gene            3576..4268
                     /locus_tag="VFU72_11455"
     CDS             3576..4268
                     /locus_tag="VFU72_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424149.1"
                     /translation="MATRILIVDDHALVRDGTRQLLEKEPELEVVGEAGRAEEALELA
                     RRLQPDLVLLDLALPDLNGIEVARRLREITPQTKVIVLSAYTNETYVRTALEAGAVAY
                     LPKTVRGQEVIEAIAAVRGGQVILHPTVAATLQHALQRGGSGRPALSAREREILQLAA
                     RGASNKAIAEELFLSVRTVESHLSHTLAKLGVASRAEAVAYGVSQGWISFDEAAISAE
                     PAAPDAERAHRD"
BASE COUNT          596 a         1543 c         1512 g          729 t
ORIGIN      
        1 ccccggcggc gctcaagcac cagttgggtg cgacgctgca tgccgacgat ctcgctgcgg
       61 cgcgcgccgc gctgctcgcg ttggcggatc tggtcgagca cgacatggcg gcattcacgg
      121 atagccgggc gcagctctcg ccggaacaac gtgcggtgct ggccggcgtg cggagccggt
      181 tgagcgggat caatccatag cagtgaaccc ccttccactc actgaattga gccggcgcga
      241 gcgcctgggc gtcccgctca ccatgtgccc catcctccgc acctatacgc ccagtcattc
      301 cgtttaggcg taatccgtgt tactctcaac cggaaggagg aagacgtgtt tgacgatctc
      361 gtgctcaacg taccgctgct gcggcggcgc gtgccgaatc tgaccgccgc cgcgcgctcg
      421 gtcgggctgc ggccggccac cgtctccaat ctctgcaccg gcaagatccc gatcggccgc
      481 gccgaggtgc acacgctggt ggccctggcc actctggccg gctgctcgct ggatgagctg
      541 gtgctgcgcg gcgggctcgg gggccagatc gagacggggg tcaaagtact cgacctggcg
      601 gcgccgctgg tgcggggagg cacggtcggc ctcgtcggac gggcggggct gggccaactg
      661 gtcctgatca tggagctgct ctaccggctg cggacccggc ccgacttcgc caccatcctc
      721 tggttgccgg ccgagccgct gcccgccgcc gatgaggtct tgccccaggc gagcgcgacc
      781 ggcacgaccc tggacgaggt ctacgggctg gtcgaggcgt cccgcgccga gcgcgacgtg
      841 ctgctggccg ccgatcgcac gacggttctc tcgggagaac tgctggcact gcgcacgcag
      901 ctccaagagc cgggcagccg gccgatcacc gtggcgctct tcgatctgcg cggcgaggcc
      961 tcgatcgagg aagcgcccta cgggccgctg gatactcgct gggtcttcga tcccgatctg
     1021 gccgcccgtc gcctctaccc agccgtggac ccggtcagct ccacctcggt gctgctcgaa
     1081 ggcgcacagt tggaggccag ccacctcacg ctggcggcgc ggacccacgc cctgctgcgg
     1141 cgctatcgcg agctgcgccc actggtaccg gtgcacgggc tggagcggtt tccacccgcc
     1201 gagcagctga ccttccagcg gggcgagcac ctggaggcgt tcctgtcgca gcccctcttc
     1261 gcggccgagg cccacaccaa gcgcccagga cgctgggtct cgctggccga aacgctggcc
     1321 gacgtgcgcc gaatcctgga cggcggagcg gacacgctcc cggtcgacgc gctgcacgac
     1381 atcggcgggc tcgtcgaggc cgaaggtcag agttgagccg gttgccatac gagcaggagt
     1441 gaccggcatg ggtaccgccc gagctggaga ggagcggcgg catcttccac tctatgccct
     1501 gctgaccggc accggcgtct cgatcgtcgg ctcgctgatg acgctgatcg cggtgccctg
     1561 gttcgtgctg cagacgaccg gcagcccggc caagaccggc atcacgactg ccgtctgggt
     1621 cctgccggtc atcctctccg gcgcgctggc cggcccgatc gtcgatcgtg tgggccacaa
     1681 gcggatcggc gtggtcggcg acctcggcgc cgggctcagc gtggcggcgg tgccgacgct
     1741 ctatcacacg gtgggattgc cctttgccgc gctcctactg atcgtcttcg tgcgggggct
     1801 ggccgacttt cccggccgca ccggccgtcg cagcctggtg cctcagctgg cggagctggc
     1861 cggctggccg ctggagcggg ccaacgcggc cttcgccact atccagaacc tggcgctgct
     1921 gctgggcccg gcccttgccg gtgtgctgat cgcccgcttc agcccgagta acgtcatgtg
     1981 gctcgacgcg gcgacctatg gcttctccgc cgccatgacc gcgctcttcg tgcccagacc
     2041 tgctcccgag cggacagcca ccgacccggc ggagcgctat cgcgagcgcc tgctggacgg
     2101 ctaccgcttc gtgcgccggg accggctgct gttgacctta ctcggcttca ccaccctcaa
     2161 caccctgctc acctcgccgc tgttcgacgt ggagctgccg gtctatgccc ggcaggtctt
     2221 cgatagcgcg gtggcgctgg ggctgatgtc cgccggctac ggtgccggcg cgctggcctc
     2281 ctccttcctc tacggcgcct tcggcgagcg gctgccgcgc cgggcaatgc tgctgggatc
     2341 cgtcgtgctc accgcgctcc cgctgggcct gctcacgctg gagcccccgc tgccgctggc
     2401 gatgctggcg ctagggctga gcgggttcgc ggctggcccc gttgggccgc tctatcagac
     2461 cgtggtacag gagcgtgtcg gctcggccat gcgcgggcgt atctttgggc tcagcggggt
     2521 cacgttcgtg gctatgccgc tcggagtgct ggcggccggc ttcctgattc agggcttcgg
     2581 ggtgcggctg gcgctggtgc tgcaggccgc ctgcatcctg gccgcggcgc tcatcctcag
     2641 ccgcctgccg gccctgcacg cgctcgacca cgccgccgag gccgacgaga tcgcggtgag
     2701 acagctgtaa tggaaggcgc cgccgctatc ctggccttca ccctggctgc cttccgcctc
     2761 ttcccggcgg tgcggcgcat gccgtagccc tcgtcaggtc gaacgtcagc cacgcacgcg
     2821 agcgagcggg cttctcgccc gcccgctcag gaccacgcga cggatgatcg atcaggactt
     2881 gggcgcctgc gagtccggca ggacgatgag ctgaccccac atgtagggcg agtgctcgtc
     2941 ctcggcgcag acgaaggtgt agatccccgc cttaccggcc acgaagctgg cgtgggaact
     3001 cgactgtcca ggcatctggg cgttgacatt cagatcgctg atggtgaagg tgtgtgcgtc
     3061 atccgccggg ttgaccagcg tgagattgac ggagtcgccc tggtagacga ccagcgtgct
     3121 cggtgagaac gcatacacct ctttgccgtc gagcagcccg cccggcgcga agtccttctg
     3181 caggtaatcc agggtcccct gcatctcgtg caccagcgtc gggaccgcca ccaccgtgaa
     3241 atcgcgcgtc tggggctggt aggtgcttcc cttggcagtg accgtgaacc cggtcgtaat
     3301 cagcgctacc agcgcgagca ttgccgccgc tccggctatc cagcgccgcc gcctcgtggt
     3361 gaacatctcc gtctcctttt ctccttggcg ttaccgcccc catgccatcg aatctgcccg
     3421 tagtctgcgc ctcgggcggg cggcggtcat cggcggtgcc acggatttgc actccgcgta
     3481 ttcgcggagt tgaatctcag tgaaatcact gatgaccgcg cgcgcggtga aggagcacac
     3541 tgtcccccgg gaggggggcg aaaggcagtc acgggatggc gacacgcatc ttgatcgtcg
     3601 atgaccacgc cctcgtgcgc gacggcaccc gccaactgtt ggagaaggag cccgagctcg
     3661 aggtggtcgg cgaggcgggc cgggctgagg aagcgctcga gctggcgcgg cgcctccagc
     3721 ccgatctggt gctgctcgat ctggcgctgc cggacctcaa cggcatcgag gtcgcgcgcc
     3781 ggctacggga gatcacgccc cagaccaagg tgatcgtcct cagcgcctac accaacgaga
     3841 cgtatgtccg cacggcgctg gaggccgggg ccgtcgccta cctgccgaag accgtgcgcg
     3901 ggcaggaggt catcgaggcg atcgcggcgg tgcggggtgg ccaggtgatc ctccacccca
     3961 cggtggcggc cacgctgcag catgcgctgc agcgcggcgg atccggccgg ccggcgctct
     4021 cggcgcgcga gcgggagatc ctccagttag cggcgcgcgg cgcctccaac aaggcgatcg
     4081 ccgaagagct cttcctgagc gtgcgcacgg tcgagagcca cctgagccat accctggcga
     4141 agctgggggt ggcatcgcgc gccgaggcgg tggcctacgg ggtttcgcag ggctggatca
     4201 gcttcgacga ggccgcgatc tccgcggagc cggcggcgcc cgacgcggag cgggcgcatc
     4261 gtgactgacc agcacacctc cctgcccgcg ccggcccccg ctcggctcca actgctgcgg
     4321 tcgctgcggg cgctaggagc tgagctacgc cggcatctgc acgaccggcg cttctgggtc
//
LOCUS       DASUBI010000592         5806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1160118, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000592 DASUBI010000000
VERSION     DASUBI010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160118"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFU72_11460"
     CDS             <1..181
                     /locus_tag="VFU72_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEU5424150.1"
                     /translation="LAVFGYPISEEFQEQNPADGKMYTVQYFERARFEYHPEFKGTPY
                     EVELGLLGRQTMPRR"
     gene            complement(376..1614)
                     /gene="fabF"
                     /locus_tag="VFU72_11465"
     CDS             complement(376..1614)
                     /gene="fabF"
                     /locus_tag="VFU72_11465"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5424151.1"
                     /translation="MDRVVVTGVGAVTPIGNDAQTFWKNLMAGVSGIDRITAFDSSNL
                     EVRIAAEVKDFDPKQFIDFKDARRMDRFSQFAVVAAGEALRDARLEITPEESERVGVM
                     INTGGGGIPALTREVLTHHTKGPRRVSPFFIPMFAPNMAACQVSITYDIHGPVMASVA
                     ACAAGMQAFVDALRMLRLGEVDVIIAGGTEAGVDPVSVAAFGNMGALSKRNDDPQHAS
                     RPFDRDRDGFVFGEGCGVLILEREGHARERGARVYCELAGGAMTADAFHISAPKPGGE
                     DQARAMRLALANAHIQPEDVDYICAHGTSTPLNDGIETAAIKQVFGPHAYELAVSSPK
                     SMIGHLVGAAGAVSGVVCALSIRNNMVAPTINLENPDEGCDLDYVPHEARHMQVDVAL
                     ANAFGFGGQNAVAVFRRYQG"
     gene            complement(1641..2591)
                     /gene="fabD"
                     /locus_tag="VFU72_11470"
     CDS             complement(1641..2591)
                     /gene="fabD"
                     /locus_tag="VFU72_11470"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5424152.1"
                     /translation="MGLGTAISKRIAFVFPGQGSQYVGMGRGLYEASAGARRIFQQAD
                     EILGFSLSSLCFDGPAEELDDTINAQPAILTVSLAYLEALRERWHALGESVAPVYVAG
                     HSLGEFTALVAAGAIDFGDALRLVRERGRLMKESGDLRPGGMAAVIGLGREELEAVCV
                     EAGERGIVVLANDNCPGQSVISGDVGALERAMELARLRGARKAVRLGISIASHSPLME
                     RAGQQFNDLVGRIHLRDPQVPVIANISGQVLTTAEEIRREIAHHVVRPVQWTGSVREM
                     LNDGVSTFLEIGPGNVLGGLIKRITSDVEIVSVKDFGLGA"
     gene            complement(2803..3561)
                     /locus_tag="VFU72_11475"
     CDS             complement(2803..3561)
                     /locus_tag="VFU72_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU5424153.1"
                     /translation="MTDSDVSSETARRSPDRRAAPAGTGAPAPEGYSLWFVLIAAIFV
                     TTLITSNIIAVKLVDVAGLVVPAATVTIFPLSYLLGDILTEVYGFSRARQIIWLGFLC
                     NLMAVAAIYATQLLPAPGFWDAQVAYERILGFAPRLLAGSFAAYLVGEFANAYVLARM
                     KVLTNGRWLWSRTISSTLIGEGIDSLVFISIAFAGTITGHQLLRTMATAWLFKSGYEV
                     LATPLTYLVVNFLKRSEGVDVFDRQTDFSPVRFS"
     gene            complement(3554..5524)
                     /gene="ftsH"
                     /locus_tag="VFU72_11480"
     CDS             complement(3554..5524)
                     /gene="ftsH"
                     /locus_tag="VFU72_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5424154.1"
                     /translation="MREDGRRPNPGQQPPQPTRSRFPTWILWVVLIGLALYNIYVYAW
                     PSSAARVTVPYSVLVSQVQGDNVQKVTIKGQSVSGDFKQEIQLVNGQVLGPNEPLPSG
                     ATEKDVSKGTKFQSTIPENTQDQFTALLQQHNVQINVEQNTGSILPTLLTLGLPLLLF
                     IGLMIYLGRNVSRGQQNVFSFGRSKARMYDIERPRVTFADVAGEEESKAELSEVVDFL
                     RNPGKYHSIGARLPRGILLVGPPGTGKTLLARAVAGEAGVPFFSVSASEFVEMFVGVG
                     ASRVRDLFEKAKAASPSIIFVDELDAVGRQRFAGVGGGNDEREQTLNQLLVEMDGFDA
                     NHDVIVIAATNRPDVLDPALLRPGRFDRQVTVGLPDRRGREAILKIHSRGIPISNEVD
                     LGAIAAATPGFSGADLANLVNEAALMAARKNKKVVEQIDFDEAIDKIVLGTERALLMS
                     EHDKQVVAYHEAGHAIVAYFSPGADPLRKVSIVPRGRALGVTVQSPTEDRYNYSRHYL
                     LGRLAVMMGGRVSEQLVFNEMTTGAQNDLKEATDLARRMVGLWGMSEEVGPIYLGTGE
                     QHVFLGREMGQERNISDDLLDRADSATYKLVEGAMNEAERILTAQRDKLDDLAAVLVK
                     DETIGQERIAELLGVPPVPAAAPLAAGDGADD"
BASE COUNT          903 a         2003 c         1957 g          943 t
ORIGIN      
        1 cctggccgtc ttcggctatc cgatcagcga ggagttccag gagcaaaacc cggccgacgg
       61 caagatgtat accgtgcaat acttcgagcg cgcccgcttc gagtaccacc ccgagttcaa
      121 gggtacgccc tacgaggtgg agctgggcct gctgggccgg cagacgatgc cgcgccgctg
      181 aatgatggcc taatcacaac gctgtaggga cagacccctg tgtctgccct gctcccccca
      241 cgcctcggca acggggaatg cgacatcgtc ggtggcacac gacgacgcca gtggtaggcc
      301 acgcgggcag acacaagggt ctgcctctac agggaaacgc gcccagccaa cgacgtggga
      361 accggccctc gcgccctacc cctggtaccg ccggaacacc gccaccgcgt tctggccgcc
      421 gaagccgaag gcgttggcca gggccacgtc gacctgcatg tggcgcgcct cgtgcggcac
      481 gtagtcgagg tcgcagccct cgtccgggtt ctccaggttg atcgtcgggg ccaccatgtt
      541 gttgcggatg gacagcgcgc agaccacgcc ggagacggcc ccggccgcgc cgaccaggtg
      601 gccgatcatg ctcttgggcg agctgaccgc cagctcgtag gcgtgcgggc caaagacttg
      661 cttgatggcc gccgtctcga tgccgtcgtt gagcggcgtg ctggtgccgt gggcgcagat
      721 gtagtcgacg tcctcgggct ggatatgggc gttggccagc gccagccgca tggcccgcgc
      781 ctgatcctcg ccacccggct tgggcgcgct gatgtggaag gcgtcggccg tcatcgcgcc
      841 gccggccagc tcgcagtaga cgcgggcgcc gcgctcgcgc gcgtgcccct cccgctccag
      901 gattagcacc ccacagccct cgccgaagac gaagccgtcg cggtcgcggt cgaaggggcg
      961 gctggcgtgc tgggggtcgt cgttgcgctt ggagagcgcg cccatgttgc cgaaggccgc
     1021 caccgagacc gggtccaccc ccgcctcggt cccgccggcg atgattacgt cgacctcgcc
     1081 caggcgcagc atgcgcagcg cgtcgacgaa ggcctgcata ccggcggcgc aagctgccac
     1141 cgaggccatc accggcccgt ggatgtcgta ggtgatcgag acctggcaag cggccatatt
     1201 gggcgcgaac atcgggatga agaaggggct gacccggcgc gggcccttgg tgtgatgggt
     1261 cagcacctcg cgcgtcagcg ccgggatgcc gccgccgccg gtgttgatca tcaccccgac
     1321 gcgctcgctc tcctcgggcg tgatctccag ccgcgcgtcg cgcagcgcct cgcccgccgc
     1381 caccaccgcg aactgggaga agcggtccat gcggcgcgcg tctttgaagt cgatgaattg
     1441 cttggggtcg aagtccttga cctcggccgc gatccggact tcgagattgg aggaatcgaa
     1501 cgcggtgatg cggtcgatgc cggagacgcc ggccatcagg ttcttccaaa acgtctgcgc
     1561 gtcgttgccg atcggggtaa cggcccccac cccggttacg accacacggt ccacggtccc
     1621 tcctctattc gctcgcggtc tcaggcgccg agcccgaagt ctttgaccga gacgatctcg
     1681 acgtcgctgg tgatgcgctt gatcaggcca ccgagcacgt tgccggggcc gatctccagg
     1741 aaggtgctga cgccgtcgtt gagcatctcg cgcaccgagc cggtccactg caccggccgc
     1801 actacgtgat gcgcgatctc gcgtcggatc tcctccgccg tggtcagcac ctggccgctg
     1861 atgttggcga tcaccggcac ctgcggatcg cgcaggtgga tgcgcccaac cagatcgttg
     1921 aattgctgcc cggcgcgctc catgagcggc gagtgcgagg cgatcgagat gccgagccgt
     1981 accgccttgc gcgcgccgcg cagccgcgcc agctccatgg cccgctccag cgcgcccaca
     2041 tcgcccgaga tgaccgattg gccgggacag ttgtcgttgg ccagcaccac gatgccgcgc
     2101 tcgccggcct cgacgcagac cgcctccagc tcctcgcgcc ccagcccgat cacggccgcc
     2161 atgccgcccg gccgcaggtc gccgctctcc ttcatcagcc gcccgcgctc gcgcaccagg
     2221 cgcagcgcgt cgccgaagtc gatcgccccg gccgccacca gcgcggtgaa ttcgcccagg
     2281 ctgtgcccgg ccacgtagac gggcgcgaca ctctccccca gcgcgtgcca gcgctcgcgc
     2341 agcgcttcga ggtaggccag gctcaccgtc aggatggccg gctgggcgtt gatcgtgtcg
     2401 tccaactcct cggcgggccc gtcgaagcag agcgaggaga gcgagaagcc gaggatctca
     2461 tccgcctgct ggaagatccg ccgcgctccg gccgaagcct cgtagaggcc gcggcccatg
     2521 ccgacgtatt ggctcccttg accgggaaag acgaaggcaa tccgcttaga aatcgccgtg
     2581 ccgagtccca tacccgatcg ctctctcctg gcaacgccgc ccaggaacgc tgagcagccg
     2641 aaaatcgctc cccccgagcg caccgcattc taccacgcgc ctttggcgca cttcgcccgc
     2701 cgccacgaat cgccctgctc gcgccccttc tccctgcatt gggctccgtt cgagaagggg
     2761 cgggggatga gggctcctcg ctgggggcga gtgccgcccg cctcaactga agcgcaccgg
     2821 cgagaagtcg gtctgccggt cgaagacgtc gactccttcc gagcgcttga ggaagttgac
     2881 caccagatag gtcagcggcg tcgccagcac ctcgtagccg ctcttgaaga gccaggccgt
     2941 ggccatggtg cgcagcagtt ggtgcccggt aatcgtaccg gcaaaggcga tgctgatgaa
     3001 gaccagcgag tcgattccct cgccgatgag cgtcgagctg atggtgcggc tccagagcca
     3061 gcgtccgttg gtcagcacct tcatgcgggc cagcacgtag gcgttggcga actcgcccac
     3121 cagataggcg gcgaaggagc cggccagcag gcgcggcgcg aaaccgagga tgcgctcgta
     3181 ggccacctgg gcgtcccaga agccgggcgc cggcaggagc tgcgtggcgt agatcgccgc
     3241 caccgccatg agattgcaga ggaagccgag ccagatgatc tggcgcgccc ggctgaagcc
     3301 gtagacctcg gtcagtatgt cgccgagcag atagctgagc gggaagatgg tgacggtggc
     3361 ggccggcacc accagcccgg ccacgtccac cagcttgacc gcgatgatgt tcgaggtgat
     3421 cagggtggtg acgaagatgg cggcgatcag cacgaaccag aggctgtaac cctccggcgc
     3481 cggagcgccg gtcccagccg gcgcggcgcg gcggtcgggt gatcgccgcg ccgtctcgct
     3541 tgagacgtcc gaatcagtca tcggcgccgt cgccggccgc cagcggcgct gccgccggga
     3601 ccggcggtac gccgagtagc tcggcgatgc gctcctgccc gatcgtctcg tccttgacca
     3661 gcacggcggc caagtcgtcg agcttgtcgc gctgcgcggt caggatgcgc tcggcctcgt
     3721 tcatcgcacc ctcgaccagc ttgtaggtgg ccgagtcggc acggtcgagc aggtcgtcgc
     3781 tgatgttgcg ctcctggccc atctcgcggc ccaggaagac gtgctgctca ccggtgccga
     3841 ggtagatcgg gccgacctcc tcgctcatac cccagagccc gaccatgcgc cgggccagat
     3901 cggtggcttc tttcaggtcg ttctgcgcgc cggtggtcat ctcgttgaag accagttgct
     3961 cggacacccg gccgcccatc atcaccgcca gccgcccgag caggtagtga cggctgtagt
     4021 tgtagcgatc ctcggtcggc gactgcacgg tcacgcccag cgcgcgcccg cgcggcacga
     4081 tgctgacctt gcgcagcgga tcggcgccgg gcgagaagta ggccacgatg gcgtgcccgg
     4141 cctcgtggta ggccaccacc tgcttgtcgt gctcgctcat caaaagcgca cgctcggtgc
     4201 cgagcacgat cttgtcgatc gcctcgtcga agtcgatctg ctcgaccacc ttcttgttct
     4261 tgcgcgccgc catcagggcc gcctcgttga ccaggttggc caggtcggcg cccgagaatc
     4321 cgggggtggc cgccgcgatg gcgcccaagt cgacctcgtt cgagatcggg atgccgcgcg
     4381 agtggatctt caggatcgcc tcacggccgc ggcggtcggg caggccgacc gtcacctggc
     4441 ggtcgaagcg gccggggcgc aagagcgccg ggtcgagcac gtcgggccgg ttggtggccg
     4501 cgatcacgat cacgtcgtgg ttggcgtcga agccgtccat ctcgaccagc agttgattca
     4561 gcgtctgctc gcgctcgtcg ttgccgccgc cgacgcccgc gaagcgctgg cgtccgaccg
     4621 cgtccagctc gtcgacgaag atgatcgagg gcgaggccgc tttggctttc tcgaagaggt
     4681 cgcgcacgcg gctggcgccg acgccgacga acatctcgac gaactccgag gcgctgacgc
     4741 tgaagaaggg cacgccggcc tcgccagcca cggcgcgcgc cagcagcgtc ttgccggtgc
     4801 ccggcgggcc gaccagcagg atgccgcgcg gcagccgcgc gccgatgctg tggtacttac
     4861 cggggttgcg caggaagtcg accacctccg agagctcggc cttggactcc tcctcgccgg
     4921 ccacgtcggc gaaggtcacg cgcgggcgct cgatgtcgta catgcgcgcc ttgctgcgac
     4981 cgaagctgaa gacgttctgc tgcccgcgtg agacattgcg ccccaggtag atcatcaggc
     5041 cgatgaagag cagcagcggc aggccgagcg tgagcagggt cggcaagatc gagccggtgt
     5101 tctgctccac gttgatctgc acgttgtgct gctgcaggag cgccgtgaac tgatcctggg
     5161 tattctccgg gatcgtgctc tggaacttgg tgcccttgct gacgtccttc tcggtcgcgc
     5221 cgctgggcag cggctcattg gggccgagca cctggccatt gacgagctgg atctcctgct
     5281 tgaagtcgcc gctgaccgac tgccccttga tggtcacctt ctggacattg tcgccctgca
     5341 cctggctgac cagcaccgag tagggcacgg tgacgcgcgc ggcgctgctg ggccaggcat
     5401 agacgtagat gttgtagagc gcgagaccga tcagcacgac ccagaggatc caggtcggga
     5461 agcgggatcg ggtcggctgg ggtggctgtt ggccggggtt cggccgcctg ccgtcttctc
     5521 tcacgatgca ccttcctcat acggctggac ggcgcggctc gcgcctgacc tgccgcgccg
     5581 gttgccgcgg cggaggtgcc agcccgccca tacgtgtatt gtagcccgtg gccggggctg
     5641 gctgccggaa agcgcacgag gcagccgctg gtggacggct gcctcgcacg tgaggaggag
     5701 atgagtcttg aaatcgatgc tgataccgcc gggagacgct gtcacgccgc tcgactggct
     5761 ctattctctc cctttttagt cgttttgtct acccctaaac gaccca
//
LOCUS       DASUBI010000593        14071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1336838, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000593 DASUBI010000000
VERSION     DASUBI010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336838"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFU72_11485"
     CDS             complement(<1..505)
                     /locus_tag="VFU72_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424155.1"
                     /translation="MAPASAPRAPAPPLPLTFGRRATLIDLGLLTVFWLLYFWPILVT
                     RSALIPYDLLDQHYMFQAFVHRALASGQTPLWTPNILSGYPIAADPLTQLFYPPNLLM
                     HLLSPGAFLPYLALEWQATLHFLFAAFGTYFLARALAGSRTGALLAALVFAFGSFFAW
                     HLPHLSPI"
     gene            complement(561..1856)
                     /locus_tag="VFU72_11490"
     CDS             complement(561..1856)
                     /locus_tag="VFU72_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424156.1"
                     /translation="MEIVEILGASPRSTRLTCARLTWRGSLALIAILAVSLLAGCSGP
                     ESPSTAGASPASALSASGVATPLSTPAAATISPGPPAPSPASPGTPAPGSTFAYGFNV
                     FVRGDDSGAAFNDKTFAMVNGAGFDWIHFQLSWKQFEPNKGQWDPLPTDRIVKQALDA
                     KIHILVSVVDFPDWARDPSNKQLLQDWSDYQTFVHFLADRYRGQINVWEIGNEENLAY
                     TVGGTVRLTDYAHLLEAGYQGVKSAAPDALVLFGALTPTGINNPTIAIDDVQYLKQFY
                     ALQGGYYTRFFDALGMHANATKNAPDLMWPGNPGKDGWSDDGSFYFRRVEQLHQVMQE
                     HNDTRPAWITEFGWTTENQAPGYEYGKFVTPQDQASYLVRAFQIARQSWPWCAGMFVW
                     NLNYSVVSAPSDEKTPWAVLNADWSPRPAYTALKNMPKP"
     gene            complement(1973..3709)
                     /locus_tag="VFU72_11495"
     CDS             complement(1973..3709)
                     /locus_tag="VFU72_11495"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU5424157.1"
                     /translation="MRVFKLVIVMGMLAALLLPLGAAQAAPSSPQDNLMPRYFPETGY
                     WVQGVFRQYWETHGGVYIQGYPITAVFQQDGMWVQYFERARFEWHPDNPDPWKVELTL
                     LGSLRTANRHGEQPFQPIPAFANSNDHVYFDATHHALSYGFKTFWNDNQGIQNFGYPI
                     SEEFTEKNQPPPAGDGQDHTVQYFERSRFEYHPENKGTKYEVLLGLLGREYLAQRGAP
                     PEAVARQDPNMPPPDLITGALYAPHAAAGFNVYLRGDDSGQSFNDQALNLVTNAGFSW
                     VHFQLPWSSIEPQPGVYDAQGPDRIINQVASKGLKMLVSVVGPPPAWAGTSGIPDDTS
                     HYQALVKYLAARYQGKVQAWEVWNEENLAYTVGGQVDVGKYVNLLKAGYQGIKSSDPH
                     AIVLFGALTPNGVNDPSIAIDDVQFLKQVYAYNNGEVKNYFDALGAHPGSNNNPPDTM
                     WPNNPGPGPGWQNDPSFYYRRIEQLRQVMIDNQDAAKQIWLTEFGWTTANQAPGYEYG
                     AQNSDQDQANYLVRAFQIGQTEWPWVGPMFVWNLNYSVVSPPSDEKTPWAVLNADWSP
                     RPSYTALKNMPK"
     gene            complement(3906..4355)
                     /locus_tag="VFU72_11500"
     CDS             complement(3906..4355)
                     /locus_tag="VFU72_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5424158.1"
                     /translation="MLTALPFEWFVLLLRLLFIFLIYFFLFQVVRVAIRELTVAGMGS
                     EEPVDEVGGVLVVREPGGSRLKRGDRIELDPVTLIGRTPRATIRLADSFVSGEHAQVD
                     TRDGRWWLTDLQSTNGTLLNGHPVTGRTGLRYGDVISIGDVQLQLAR"
     gene            complement(4371..5126)
                     /locus_tag="VFU72_11505"
     CDS             complement(4371..5126)
                     /locus_tag="VFU72_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEU5424159.1"
                     /translation="MVGSLQHFERFFERLMEGAVGRIFRSPVQPAEIGRRLERAMDDN
                     QLATVDGPVVPNDYTVHLNPDDMVLFADFTTTLCRQMEDWLIDLADERNFGFVDQVRV
                     QMVGDERVAPRGIMVDAHIAELPGYSPEEQEAVQRTEVYRVVEQTGSVPPKLLQVING
                     VNANEVFVVRLQVTSIGRALDNDIVLSEAEVSRHHARIEYQNGTFEVVDLGSTNGTSV
                     NGVPVSRGRLHDGDVIGLGTARLELIPYGSAGD"
     gene            complement(5204..5977)
                     /locus_tag="VFU72_11510"
     CDS             complement(5204..5977)
                     /locus_tag="VFU72_11510"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEU5424160.1"
                     /translation="MTAPHRLVDLHCHVLPNVDDGADDDEMALRMLQASAMSGVARVV
                     ATPHAHHCPAELIPRLVERLNVKARRAGLEIEVLPGQEVRLGAHVVERLVAGELLTLN
                     QTRYLLLELPLSGSWPSYLEAVVDDLQARGYRPILAHAERYPDVQRNPRLVEPLVARG
                     VPVQLNADSLGERSERGARPAAEYLLAHRLAHLIASDAHSDSWRPPRLDAALARAAEL
                     AGASYSAWMIEASWSIVRGEPVELPEPCAAAEPEEGRVV"
     gene            complement(6111..7040)
                     /locus_tag="VFU72_11515"
     CDS             complement(6111..7040)
                     /locus_tag="VFU72_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424161.1"
                     /translation="MERHRHIGYQGLLGLLLLLVCWPLAWLGWEPVSRYSFFPLWLGF
                     VLTLDGLNEWRSRTSLWRRGKRHFVLLFAISAPFWWIFEWVNRYLRDWHYVTPLNHSL
                     LVYALLATLDFSTVIPAVLEMTELFASFRLGSRLPRIPAWRVSPVGITVLEALGWFML
                     LLVILLPDYFFPLTWIALFLIVEPFNLAVGQRSVGWFLARGESSALWNLMLGALATGW
                     FWEMWNYYSLPKWYYTVPYVDFWHVWEMPLLGYGGYLPFGLEVMSVFALGFWLLERRP
                     QRYTRIVSRQAAETARVGADGREHAPAPARSRP"
     gene            7111..7791
                     /gene="rpe"
                     /locus_tag="VFU72_11520"
     CDS             7111..7791
                     /gene="rpe"
                     /locus_tag="VFU72_11520"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5424162.1"
                     /translation="MPEHARSGTIAIAPSILTADLANLAREVERVEAAGADYLHLDVM
                     DGVFVPNISFGAPVVAAVRQITRLPLDVHLMIVQPERYVEQFAAAGADSITVHVEACT
                     HLHRTVQQIHEAGARAAVALNPATPLVMLEDILPYLEMILLMSVNPGFGGQEFIPTTL
                     EKIRRLRRILDERGSGALIETDGGVKADNLRAVAEAGADIAVVGSGVFNPTQTVEEAM
                     RALRDAVG"
     gene            complement(7835..8275)
                     /locus_tag="VFU72_11525"
     CDS             complement(7835..8275)
                     /locus_tag="VFU72_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424163.1"
                     /translation="MLPDLAVGGLGFGLVVAPIGAAAINAARQEDLGIASALVIVMRL
                     LGMTLGISILTGWAVRRLNEALTRLPPVVQQPGESVGQYLARQEAFAQEHAIPLTLGI
                     IRDTYGAAAVICVGALVPATFLFMRRGQRAGQRAEEHERYSRSG"
     gene            complement(8300..9424)
                     /locus_tag="VFU72_11530"
     CDS             complement(8300..9424)
                     /locus_tag="VFU72_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424164.1"
                     /translation="MTSAPSRQKSPGTLAALVPALVVVFVAALDLTVIAPLLPKMLFD
                     LQINTAEADRYVWIVSGYLIAYTVSIPLMGRLSDRIGRRATFLLALLVFLAGSAVSAS
                     AHSLEALIAARAVQGIGGGAMVPVAMALVGDLLPPEERAGALGIVAAVDTLGWVLGPI
                     WGAAIDAVFSSWRAVFWLNLPIGLLAVVGLVFFWRHQPAPRARETGGRPDVLGALLLT
                     LGLVAINLGFASSAGGASGETLALGSSPNPLEPYRIPLIVIGVVSLLALVFVELRASR
                     PLIPMALFRDRLFSAANATNVLVGAGLMVALVNVPLLVSLLENADRATTVSAELLGAF
                     SLTMAIGAWLGGRATRLVGYRPLIWIGLALAAVGFVSMSN"
     gene            complement(9424..10212)
                     /locus_tag="VFU72_11535"
     CDS             complement(9424..10212)
                     /locus_tag="VFU72_11535"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEU5424165.1"
                     /translation="MKLHSYRLASLALVLFLSLGLAAACRGKSKPSGTEAAATPTASP
                     TPSPTPTPTPTAQGILQQASDRWNHLDSLHFNLQIEGVVALDSTGLVQLHSADGDLKR
                     PGLAQATAKVSVAGSNLNMKMISLGTDQYITNFITGRWEKAPADLGYNPAVLFDKDHG
                     VGAVLTSIQNPAIAGSESIDGQEAWHITGTVTKAAASAIVGGALHGDTATVDVWVSKS
                     THDLLKLVVHDPGAATPSAAQGAATWTLTTSKQNQPVTIERPNV"
     gene            10382..11257
                     /locus_tag="VFU72_11540"
     CDS             10382..11257
                     /locus_tag="VFU72_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5424166.1"
                     /translation="MKVVVGAASDTGTVREQNEDAILVETESAADGSVEQSMLLAVAD
                     GMGGYQRGEVASQIAIETLEKEYRGGPLAPTEAPQRLKRAFLNANDLIFRDGSAEGEE
                     HMMGTTLVAAVIQGHDLTIANVGDSRAYLVRANRATQVTRDHSLVAEQVAAGAMTEQE
                     ARESQHRNIITRALGHRQRVDVDVFEIRLLPEDRLLLTSDGLHDYVDETEMVQAALGR
                     PPEDAARELVNLALQHGSNDNVSAVIAWMAPAGMPTERVPEAAAARGSMLVPLIVLIG
                     VIVFIAVVVLLLYLA"
     gene            11291..12163
                     /locus_tag="VFU72_11545"
     CDS             11291..12163
                     /locus_tag="VFU72_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424167.1"
                     /translation="MTRSGPALVIDAHTHIFPPFIKDSREAHRERDVWFAELYASPKA
                     LLTTAEDLIASMDRAGITRSIACGFPWRDPGLCRAHNDYLAEAARSYPERISWLATVS
                     PGGPEALAEVERAFSLGAAGLGELNADGPNFALDQPEPLAPLVELVTAHRRPILFHVS
                     EPVGHSYPGKGTATPARLVVFLAAFPEVRVAAAHWGGGLPFYELMPEVAELTRNLVYD
                     SAASTYLYRFQIFPSAVHITGLERVMFASDYPILRQDRFLRRVRQVLAPHELEPVLWQ
                     NAARFYRLPEGELL"
     gene            12160..12741
                     /gene="ruvA"
                     /locus_tag="VFU72_11550"
     CDS             12160..12741
                     /gene="ruvA"
                     /locus_tag="VFU72_11550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5424168.1"
                     /translation="MIAGVRGRIAGRLPGTVLVDLHGLILQVLTSATTLSDIGETGSE
                     VELVTHLHVREDALTLFGFATQDELRLFLLLLGVTGVGPKAALSILSTARPAELARAI
                     EAGDADLLSRAPGIGRKTANRIILDLRGKLPEQVEGPTGPIEPADVEALEALRALGYS
                     AVEARTALSQLEPQPGQTVEQRVFAALQQLARA"
     gene            complement(12847..13833)
                     /locus_tag="VFU72_11555"
     CDS             complement(12847..13833)
                     /locus_tag="VFU72_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5424169.1"
                     /translation="MVDEREDRDEERRAQGAGPPGDLEVIEPGEAEEAEFEPEIEDLE
                     LIARYDPAAVTDPIRLYLRDIARTPLLSAQEEVELAKRVEAGDMEALQKFVRANLRLV
                     VSIAKRYLGRGLSLLDLIQEGNMGLIRAVEKYDWRRGYRFSTYATWWIRQAISRAIAD
                     QGRTIRLPVHMTDSITRYRRTVSQLTQELGRPPHPEEVAEAMSVPPEKIEQIIRAAQR
                     TSSLETPLGEEQESSLGDIIADEVSQSPEEIAAETLLQRDVSKVLEQLTPRQRLVLQL
                     RFGLNNGTPHTLAEVGEQLNISRERVRQLENEALRKLRKVGTQRLASYAAEL"
BASE COUNT         2392 a         4776 c         4654 g         2249 t
ORIGIN      
        1 aaatcgggct gagatgcggc agatgccagg cgaagaagga gccaaaagca aagacgagtg
       61 cggccaggag tgcgccggtc cggcttccgg ccagcgcacg ggccaagaaa taggtaccga
      121 aggccgcgaa caggaagtgg agtgtggcct gccactccag cgccaggtag ggcaggaagg
      181 cacctggcga gaggaggtgc atcagcagat tcggcgggta gaaaagctgg gtcagcggat
      241 cggccgcgat ggggtagccg ctcaggatgt tcggcgtcca gagcggagtt tggccgctgg
      301 ccagcgcgcg atgcacgaac gcctggaaca tgtagtgctg gtcaagcaag tcgtacggga
      361 tgagcgcgct gcgagtgacc aagatcggcc agaaatagag cagccagaag acggtcaaga
      421 gccccaggtc gatgagcgtc gcacgcctgc cgaaggttag cggtaggggc ggcgccggcg
      481 cgcgcggcgc ggaggccggg gccaacaatc cgcgcctcag ccgactacgc cgcactcgca
      541 cgtgccgccc gctcacggca tcagggcttc ggcatgttct tcagcgccgt gtaggccggt
      601 cgcggtgacc agtcggcgtt gagcaccgcc cagggcgtct tctcgtcgct cggcgcggac
      661 acgacgctgt agttcaggtt ccagacgaac atgccggcgc accagggcca actctggcgc
      721 gcgatctgga aggcgcgcac caggtacgat gcttgatcct gcggcgtcac gaacttcccg
      781 tactcgtagc ccggcgcctg gttctccgtc gtccagccga actcggtgat ccaggcgggg
      841 cgtgtgtcgt tgtgctcctg catcacctgg tgtagttgct cgacacgccg gaaatagaag
      901 ctgccgtcgt ccgaccagcc atcctttcct ggattacccg gccacatcag gtcgggcgcg
      961 ttcttggtcg cgttggcgtg catgccgagc gcgtcgaaga accgggtgta gtagccccct
     1021 tgaagcgcgt agaactgctt gaggtactgc acatcgtcaa tcgcgatcgt gggattgttg
     1081 atgccggtcg gcgtcagcgc gccgaagagc acgagcgcgt ccggcgcggc ggatttcacc
     1141 ccctggtagc ccgcctcgag caagtgcgcg tagtcggtca agcgcacggt tccgcccacg
     1201 gtgtaggcaa ggttctcctc attgccgatc tcccagacgt tgatctgccc gcggtagcgg
     1261 tcggcgagga agtggacgaa ggtctgataa tcggaccagt cctggagcag ttgcttgttg
     1321 ctcgggtcgc gcgcccagtc ggggaaatcg accaccgaca ccaggatgtg aatcttcgca
     1381 tccagcgcct gctttacgat gcggtcagtc ggcagcggat cccactgacc cttattcggc
     1441 tcgaactgct tccaggagag ctggaagtga atccaatcga agccagcgcc gttgaccatt
     1501 gcgaaggtct tgtcgttgaa cgcggctccg ctgtcgtcgc cacgaacgaa gacgttgaag
     1561 ccgtaggcga aggtggatcc cggcgcaggc gttcccggag atgccggcga cggagcgggc
     1621 gggccagggg agatcgtcgc cgcggcgggc gtcgacaacg gcgtcgcgac gcctgacgcg
     1681 ctcaacgcgc tggccgggct ggcccccgct gtactggggg actccggccc gctacaaccg
     1741 gccaggagcg agacggcgag gatcgcaatc agcgccagcg acccacgcca cgtcagccgg
     1801 gcgcacgtta ggcgggtcga gcgtggtgat gcgcccaata tctccacgat ctccatctcc
     1861 gtcacgcgga tctcgccggc cgcttccgtc gcggtcagcg cgggccgcgc aggatcaaac
     1921 gttgcggctc agcgttcgat cctgcgcacg gataccccgg ttcgagcctc cgctacttcg
     1981 gcatgttctt gagcgccgtg tacgacggcc gcggtgacca gtcggcgttg agcaccgccc
     2041 agggcgtctt ctcgtcgctc ggtggggaca cgacgctgta gttcagattc cagacgaaca
     2101 tcgggccgac ccacggccac tcagtctgcc caatctggaa ggcgcgcacc agatagttcg
     2161 cctgatcctg gtcgctgttc tgcgcgccgt attcatagcc gggcgcctgg ttcgcggtcg
     2221 tccagccaaa ttcggtcagc cagatctgct tggcggcgtc ttggttgtcg atcatgacct
     2281 gtcgcaactg ctcgatccga cggtagtaga acgagggatc gttctgccag ccggggccgg
     2341 gacctgggtt gttcggccac atggtgtccg gcgggttgtt gttgctgccc gggtgcgcgc
     2401 ccagcgcgtc gaagtagttc ttgacctcgc cgttgttgta ggcgtagacc tgcttcagga
     2461 actgcacgtc gtcgatcgcg atgctggggt cgttgacgcc gttcggtgtg agcgcgccga
     2521 agagcacgat ggcgtgcgga tcactggact tgatgccctg gtagcccgcc ttcagcaggt
     2581 tgacgtactt gccgacgtcg acctgcccgc cgacagtata ggctagattc tcttcgttcc
     2641 agacttccca ggcttgcacc ttcccctggt accgcgcggc caggtatttc acgagggcct
     2701 ggtagtggct ggtatcgtcg gggataccag aggtgccggc ccaggccggc ggggggccga
     2761 cgacgctgac cagcatcttc agtcccttgc tcgccacctg attgatgatc cggtcggggc
     2821 cctgtgcgtc gtagacacca ggctgcggct cgatgctgct ccagggcaac tggaagtgga
     2881 cccagctaaa cccggcgttg gtcaccaagt tcagcgcctg gtcgttgaag gactggccgc
     2941 tgtcgtctcc gcgcaggtag acgttgaagc cggcggccgc gtggggcgcg tatagcgctc
     3001 cggtgatcag gtccggcggc ggcatgttgg gatcctggcg cgccacggcc tcggggggcg
     3061 cgccccgctg cgcgagatac tcacgcccca gcaggccgag cagcacctcg tacttggttc
     3121 ccttgttctc tgggtgatac tcgaagcgag agcgctcgaa gtactgcacg gtgtgatcct
     3181 ggccgtcgcc ggccgggggc ggctggttct tctcggtgaa ctcctccgag atcgggtagc
     3241 cgaagttctg gatgccctga ttgtcgttcc agaaggtctt gaagccgtag ctcagcgcgt
     3301 ggtgcgtcgc gtcgaaatag acgtgatcgt tggagttggc gaaggcgggg atcggctgga
     3361 agggctgctc gccgtgacgg ttcgcggtgc gaaggctacc gagcagcgtc agctccacct
     3421 tccacggatc cgggttgtcg gggtgccact cgaagcgcgc gcgctcgaag tactgcaccc
     3481 acatgccatc ctgctgaaag accgcggtga tcgggtagcc ctggatatag acccccccgt
     3541 gcgtttccca gtactggcgg aagacccctt gcacccagta ccccgtctcg gggaagtagc
     3601 gcggcatcag gttgtcctgg ggcgacgacg gcgcggcctg cgccgcgccc agcggcaaca
     3661 gcagggccgc cagcatcccc atcacgatca cgagtttgaa aacgcgcaaa cctggctcct
     3721 ctgcagaccg gccgcgcccg gcgcccggtg ttcttgacga gtagaccact ggaatttgtt
     3781 tgccgaagac gacctccctg tccccaagcc gaatccaccg tgcgatctgg atctcgatgt
     3841 cgccgcacgg ttgatccacg cgaccgcaac cattcacgct gtggggctcc gccgcacgcg
     3901 cggcgctagc gggcgagctg caactgcacg tcgccgatac tgatcacatc accataacgg
     3961 aggccggtcc gtccggttac tggatgaccg ttcaagagtg tgccgttggt gctctgcagg
     4021 tcggtcagcc accagcgccc gtcccgcgtg tcgacctggg cgtgctcacc ggaaacgaac
     4081 gaatccgcca gccggatggt cgcgcgcggg gtgcggccga tcagcgtcac cgggtcaagc
     4141 tcgatccgat cgccgcgctt gagacgcgaa ccgcccggtt cgcgcaccac cagaacgccg
     4201 ccgacctcgt cgaccggctc ctcgctgccc atgccggcca ccgtcaactc gcggatcgcg
     4261 acccgcacca cctggaagag gaaaaaatag atcaggaaga tgaacaacag gcgcagcagc
     4321 agtacgaacc attcgaacgg gagcgcggtg agcatcgagg cgccttcgcc ctagtcgccg
     4381 gcggagccat acgggatcaa ttcgagcctg gccgtaccta acccgattac gtcgccgtca
     4441 tgcagccggc cacgcgagac tggcacgccg ttgacgctgg tgccgttggt cgagccgaga
     4501 tccaccactt cgaaggtgcc attctggtat tcaatgcgcg cgtggtgacg cgagacctcc
     4561 gcctccgaaa gcacgatgtc gttgtccagt gcgcgaccga tcgaggtcac ctggagccgc
     4621 acgacaaaga cctcgttggc gttgacgccg ttgatcacct gcaggagctt cggcggcacg
     4681 ctgccggtct gctcgacgac ccggtagacc tcggtgcgct gcacagcctc ctgctcttca
     4741 gggctatagc cgggcagctc cgcgatgtgc gcgtcgacca tgatgccgcg cggcgctacc
     4801 cgctcgtcgc ccaccatttg cacccgcact tgatcgacga agccgaagtt gcgctcgtcg
     4861 gccagatcga tcagccaatc ttccatctgg cggcagagcg tggtcgtgaa gtcggcgaag
     4921 agcaccatat catcggggtt gagatggacg gtgtagtcat tgggcacgac cgggccatcg
     4981 acggtcgcaa gctgattatc gtccatggcg cgctcgagcc ggcgtccgat ctcggcgggc
     5041 tggaccggac tacgaaagat acgaccgaca gcgccctcca tcagacgctc gaaaaagcgc
     5101 tcgaagtgtt ggaggctgcc taccaacgca aacggcccat cgctccaccg tcaccgacgg
     5161 ccacgtgaac gggccgaatt atagaggcca ccgcgaccca gggtcaaacg acgcggccct
     5221 cctccggctc ggccgcggcg cacggctcag gcagctccac cggctcgccc cgcacgatcg
     5281 accagctcgc ctcgatcatc caggcgctgt aggacgcgcc ggccagctcc gccgcgcggg
     5341 ccaacgcggc atcgagcctc ggcggacgcc aggaatcgct gtgcgcgtcg ctggcgatca
     5401 gatgggccag ccggtgcgcc agcaggtact ccgcggccgg gcgcgcgccg cgctcggagc
     5461 gttcgccgag cgagtcggcg ttgagctgca ccggcacgcc gcgcgccacc agcggttcaa
     5521 ctaggcgtgg attacgctgc acgtcggggt agcgctcggc gtgggccagg atcggccggt
     5581 aaccacgtgc ctgcaagtcg tcgacgaccg cttccagata gctgggccaa ctgccactga
     5641 gcggcagctc cagcaagagg tagcgcgtct ggttgagtgt cagcagctcg cccgcgacca
     5701 gccgctcgac gacgtgcgcg cccagccgca cctcctggcc gggcagcacc tcgatctcca
     5761 gccccgcgcg cctggccttc acgttcagtc gctccaccag ccgagggatc aactccgccg
     5821 ggcagtgatg cgcgtgtggt gtggccacga cgcgcgccac ccccgacatc gccgacgcct
     5881 gcaacatgcg cagcgccatc tcgtcgtcgt cggcgccgtc gtcaacgttg ggcagcacgt
     5941 ggcagtggag atcgaccagc cgatgcggcg ccgtcacgag tcgcgcgcca catcacgtcc
     6001 ctctcggaca ggcaaccgga tcatgcatac tccccctcga aaaaccaggc gtgacgctcg
     6061 atgatgacac aaccgtgcgc gccgcacaag ttcccagcgc cgagcgccgg ctacggccgc
     6121 gagcgagccg gcgccggagc gtgctcgcgc ccgtccgcgc ctacccgcgc ggtctcggcc
     6181 gcctgccggc tgacgatgcg cgtgtagcgc tggggccggc gctcgagcag ccagaagccc
     6241 agcgcgaaga ccgacatgac ctccagcccg aaggggaggt agccgccgta gcccagcagc
     6301 ggcatctccc agacgtgcca gaagtcgacg tacggcacgg tgtaatacca ctttggcagc
     6361 gagtagtagt tccacatctc ccagaaccag ccggtcgcca gcgcgcccag catcagattc
     6421 cagagcgcgg acgactcgcc gcgcgccagg aaccagccga ccgagcgctg ccccaccgcc
     6481 aggttgaagg gctcgacgat caagaagagc gcgatccagg tgagcgggaa gaagtagtcc
     6541 ggcagcagga tgaccaacag gagcatgaac cagccgagcg cctccagcac ggtgatgccc
     6601 acaggcgaga cgcgccaggc cggaatgcgc ggcaggcggc tgccgaggcg gaaggaggcg
     6661 aacagctcgg tcatctccag caccgccgga atcacggtcg agaaatccag cgtcgccagc
     6721 agcgcataga ccagcagcga gtggttgagc ggggtcacgt agtgccagtc gcgcaggtag
     6781 cgattgaccc actcgaagat ccaccagaag ggcgccgaga tggcgaagag gagcacgaag
     6841 tgccgcttgc cgcgccgcca gagggatgtc cggctgcgcc actcgttcag accgtcgagt
     6901 gtgagtacga aaccgagcca gagcgggaag aatgaatacc gggagacggg ctcccacccc
     6961 agccaggcga gcggccagca gacgagtagc agcagcagac cgagcaatcc ctggtagcct
     7021 atgtgtcggt ggcgttccac agtgctcccg tcagcgcgtc accggatccg gcctgtccgg
     7081 agtgcaagcg gaggaagagg cgcagtgtcg atgccagagc atgcgcgttc cggtaccatc
     7141 gccatcgcac cgtcgattct gactgccgat ctcgccaatc tggcccgcga ggtcgagcgc
     7201 gtcgaggctg ccggagccga ctacctgcac ctcgacgtga tggacggcgt cttcgtgccc
     7261 aacatcagct tcggcgcgcc ggtggtggcc gcggtgcgcc agatcacccg gctgccgttg
     7321 gacgtccacc tcatgatcgt gcagccggag cgctacgtcg agcagttcgc cgccgccggc
     7381 gccgacagta tcaccgtcca cgtcgaggcc tgcacccacc tgcatcgcac cgtacagcag
     7441 atccacgagg ccggcgcgcg cgcggcggtc gcgctcaacc cagccacgcc gctcgtgatg
     7501 ctggaggata tcctgcccta tctggagatg atcttgctga tgagcgtcaa ccccggcttc
     7561 ggcgggcagg agttcatccc caccacgctg gaaaagatcc ggcgcttgcg ccgcatcctc
     7621 gacgagcgcg gcagcggcgc gctgatcgag accgacggcg gcgtcaaggc agacaacctg
     7681 cgcgcggtcg ccgaggccgg tgccgacatc gccgtcgtcg gctccggcgt cttcaacccc
     7741 acccagaccg tggaagaggc catgcgcgcg ctgcgcgacg ccgttggctg acgagcaggt
     7801 tgcgtgcggc cccaggcgcc acacccgccc gtcgtcagcc gctccgtgag tagcgctcgt
     7861 gctcctccgc gcgctgcccc gcgcgctggc cgcggcgcat gaacaggaag gtcgccggga
     7921 ccagcgcgcc gacgcagatc accgccgccg caccgtaggt gtcgcggatg atgcccagcg
     7981 tcagcggaat ggcgtgctcc tgagcgaagg cctcctggcg cgccaggtac tgcccgaccg
     8041 actcgcccgg ctgctgcacg accggcggca ggcgcgtcag cgcctcgttc agccggcgca
     8101 cggcccagcc ggtcaagatc gagatgccca gcgtcatgcc gagcaggcgc atcacgatca
     8161 ccagcgcgga agcgatgccc agatcttcct ggcgcgccgc gttgatcgcc gccgctccga
     8221 tcggcgcgac gaccagcccg aagccgagcc cgccaaccgc cagatcgggc agcatgcgct
     8281 cgaggtcgat ggtgttgggc tagttgctca tcgagacgaa gccgaccgcc gccagggcca
     8341 ggccgatcca gatcagcggg cggtatccca ccagccgtgt agcgcgcccg cccagccagg
     8401 cgccgatcgc catcgtcagc gaaaaggcgc ccagcagctc ggcgctgacc gtggtcgctc
     8461 gatcggcgtt ctccagcagc gagacgagca ggggcacgtt caccagcgcc accatcagcc
     8521 ccgcgcccac cagcacgttg gtggcgttgg cggcgctgaa gaggcggtcg cggaagagcg
     8581 ccatcgggat cagcggccgg ctggcgcgca gctcgacaaa caccagcgcc agcagcgata
     8641 cgacgccgat cacgatcagc ggtattcggt agggctccag cggattgggg ctggagccga
     8701 gcgccagcgt ctccccgctc gcgccgcccg ccgacgaagc gaagccgagg ttgatcgcca
     8761 ccagcccgag cgtgagcaag agcgccccca gcacgtccgg tcgcccgcca gtttcgcggg
     8821 cgcgcggggc cggctggtgg cgccagaaga agacgaggcc gacaaccgcc agcaggccga
     8881 tcggcaggtt gagccagaag accgcgcgcc aggaactgaa gaccgcgtcg atcgccgcac
     8941 cccagatcgg accgaggacc cagccgagcg tatcgaccgc cgccacgatc cccaacgccc
     9001 cggcacgctc ctccggcggc agcagatcgc caaccagcgc catcgccacc ggcaccatcg
     9061 ccccaccacc gatcccctgc accgcacggg cggcgatcag cgcctccagg ctatgagcgc
     9121 tggcgctgac ggccgagccg gccaggaaga ccagcagcgc cagcagaaag gtcgctcgcc
     9181 gcccaatgcg atccgagagc cgtcccataa gcggaatcga cacggtgtag gcgatgagat
     9241 agccgctgac gatccagacg tagcggtccg cctcggcggt attgatctgg agatcgaaga
     9301 gcatcttcgg cagcagcggc gcgatcacgg tcaggtcgag cgcggccacg aagaccacta
     9361 ccagcgccgg caccagcgcg gcgagtgtgc ctggcgattt ctgccgcgag ggagcggaag
     9421 tcatcagacg ttcggacgtt cgatcgtgac cggctgattc tgcttgctgg tggtcagcgt
     9481 ccaggtagcc gcgccctggg ccgcggaggg cgtcgcggcg cccggatcat gcaccaccag
     9541 cttgaggagg tcgtgggtcg atttgctgac ccagacgtcg accgtcgccg tgtcgccgtg
     9601 cagcgcgccg ccgacgatcg cactggccgc cgccttggtg acggtgccgg tgatgtgcca
     9661 ggcctcctga ccatcgatcg actcactgcc ggcgatcgcc gggttctgga tcgacgtcag
     9721 taccgcgccg acgccgtgat ccttgtcaaa gagcaccgcg gggttgtagc ccagatcggc
     9781 cggcgccttc tcccatcggc cggtaatgaa attggtgatg tactggtccg tgcccagcga
     9841 gatcatcttc atgttcaggt tggagccggc cacgctgacc ttggccgtcg cctgcgccag
     9901 tccaggccgc ttcaggtcgc cgtcggcgct gtggagctgc acgagtccgg tcgagtcgag
     9961 cgcaaccacg ccctcgatct gcagattgaa gtggagcgag tcgaggtgat tccagcggtc
    10021 ggacgcctgc tggaggatcc cttgggcggt cggagtcggt gttggtgtcg gcgagggtgt
    10081 cggcgacgcc gtcggagtgg ccgcggcttc ggttccgctc ggcttcgatt tgccgcggca
    10141 ggcagcggcc agccccaggc tgagaaatag gaccagggca agcgacgcga gcctgtacga
    10201 atgcaacttc acgcgcgtcc tccctctccc gcgagccgca cagtccaaga tcgtatcaca
    10261 ggctgaatgc caccgattca gcgcgtttgc acgtggcggc cgctgtgcta ggattccgac
    10321 gcctgcgcca ctccgggcgg ggttgggcat gcggagcgta gaggcgcaca gcttggacga
    10381 gatgaaagtc gtcgtcgggg cggcatccga taccggcacg gttcgggagc agaacgagga
    10441 cgcgattctg gtcgaaacgg agagcgctgc tgacggctcg gttgagcaga gcatgctgct
    10501 ggcggtcgcc gacgggatgg gcggctacca gcgcggcgag gtcgcctctc agatcgcgat
    10561 cgagacgctg gagaaggaat atcgcggcgg gccgctcgca ccgaccgaag ctccccagcg
    10621 gctcaagcgt gccttcctca acgccaatga cctgatcttc cgcgatggct cggccgaggg
    10681 cgaagagcac atgatgggta ccacgttggt tgcggccgtc atccagggac acgacctgac
    10741 catcgccaac gtgggggaca gccgcgccta tctggttcgc gccaaccgcg ccacccaggt
    10801 cacgcgcgac cattcgctgg tcgccgagca ggtggccgcc ggcgcgatga ccgagcagga
    10861 ggcgcgcgag agccagcacc gcaacatcat cacgcgcgcc ctcgggcacc ggcagcgcgt
    10921 cgacgtcgac gtcttcgaga tccggctgct gccggaagac cgtttgctgc tgacatccga
    10981 tggcctgcac gactatgtgg acgagactga gatggtgcaa gccgcgctcg gacggccacc
    11041 cgaggacgcc gcccgtgaac tggtcaacct ggcgctgcag cacggctcca acgataacgt
    11101 cagcgccgtc atcgcctgga tggcgccggc cggcatgccg accgagcgcg tgccggaggc
    11161 agcagccgca cgcggctcga tgctcgtccc gctgatcgtg ctgatcggcg tgatcgtctt
    11221 catcgccgtg gtcgtgctcc tcctctacct ggcctgacat ggttccacga tccggcaatt
    11281 ggcggtatgc atgacgcgga gcggccccgc gctggtcatc gacgcgcaca cgcacatctt
    11341 tccaccgttc atcaaggaca gccgcgaggc gcaccgcgag cgcgacgtct ggttcgcgga
    11401 gctttacgcc agcccgaagg cgctgttgac gaccgcagag gatctcatcg ccagcatgga
    11461 ccgagccggc atcacccgct cgatcgcctg cggcttcccc tggcgcgatc cagggctctg
    11521 ccgcgcccac aacgactacc tggccgaggc cgctcgctcc taccccgaac gcatctcctg
    11581 gctggcgacg gtctcccccg gcgggccgga ggcgctcgcg gaggtcgagc gcgccttcag
    11641 cctgggcgcc gccggcctcg gcgagttgaa cgccgatggg ccgaatttcg cgctcgatca
    11701 gccggagcca ctggccccac tggtcgagct ggtcaccgcc caccgccgcc cgatcctctt
    11761 ccacgtcagc gagccggtcg gccacagcta ccccggcaag ggcaccgcta ccccagcccg
    11821 cctggtggtt ttcctggcag ccttccccga ggtacgcgtg gcggcggccc actggggcgg
    11881 cggacttccc ttctacgaac tgatgccgga ggtcgccgaa ctgacgcgca atctggtcta
    11941 cgacagcgcc gcctcgacct atctctaccg ctttcagatc ttccccagcg cggtgcatat
    12001 caccggattg gagcgggtca tgttcgccag cgactatcca attctgcggc aggatcgctt
    12061 cctgcggcgg gtgcgccaag tgctcgcacc gcacgagttg gagccggtcc tgtggcagaa
    12121 cgcggcccgc ttctaccggc tgcccgaggg ggagctgctg tgatcgctgg ggtccggggc
    12181 cgaatcgccg gcaggttgcc gggcacggtg ctggtcgacc tgcacgggct gatcctccag
    12241 gtgctgacct cggcgaccac gttgtccgac atcggcgaga ccggcagtga ggtcgagctg
    12301 gtgacgcacc tgcatgtgcg cgaggacgcg ctcaccctct tcggcttcgc cacgcaagat
    12361 gagttgcggc tcttcttgct gctgcttggc gtgactggtg tcggcccgaa agccgccctg
    12421 agcatcctct cgaccgcccg gcccgctgag ctggcgcgcg cgatcgaggc gggcgacgcc
    12481 gatctgctca gccgcgcccc gggaatcggg cgcaagaccg ccaaccgcat catcctcgac
    12541 ctgcgcggca agttgccgga gcaggtggaa ggaccgaccg ggccgatcga gcccgcggat
    12601 gtggaagcgc ttgaggcgct gcgggcgttg ggctacagcg cggtcgaggc ccgcaccgcc
    12661 ctgtcccagc tcgagccaca acccggtcag accgtcgagc agcgcgtctt cgccgccttg
    12721 cagcaattgg cgcgggcgtg aagaggcgct gacagtctcg ccgccagcac ccgagcagtg
    12781 ctgccatcga ggacggatcc gtgtcccacg cgtcacccaa cctcgtcgac gggcaccccg
    12841 acagcctcat aattccgcgg cgtacgaggc gagccgctgg gtgccgactt tgcgcagctt
    12901 gcgcagggcc tcgttctcca actgccgcac ccgttcgcgg ctgatgttga gctgctcgcc
    12961 aacctcggcc agcgtgtgcg gcgtgccgtt gttcaacccg aagcggagct gcaggaccag
    13021 ccgctggcgc ggggtcaact gctccagcac cttactgaca tcgcgttgca ggagcgtctc
    13081 ggccgcgatc tcctcgggcg actggctgac ctcgtcggcg atgatgtcac ccaagctcga
    13141 ctcctgctcc tcgcccagcg gtgtctctag cgaactggtg cgttgcgcgg cgcggatgat
    13201 ctgctcgatc ttctccggcg gcacgctcat cgcctcggcc acctcctcgg ggtgcggcgg
    13261 gcggcccagc tcctgtgtca gttgcgagac ggtgcgccgg tagcgggtga tgctgtcggt
    13321 catgtgcacc ggcaggcgga tcgtgcgccc ctggtcggcg atggcccggc tgatcgcctg
    13381 gcggatccac caggtggcgt acgtgctgaa gcggtagccc ctgcgccagt cgtatttctc
    13441 gacggcgcga atcagcccca tgttcccttc ctggatcaga tcgagcagcg acagaccccg
    13501 gccgaggtag cgcttggcga tgctgacgac caggcggagg ttggcgcgca cgaacttctg
    13561 cagcgcttcc atgtcgcccg cctccacccg cttggccagc tcgacctcct cctgcgcact
    13621 cagcagcggc gtgcgcgcga tatcgcgcag atagaggcgg atcgggtcgg tcaccgccgc
    13681 tggatcgtag cgcgcgatca gttccaggtc ctcgatctcg ggctcgaact cggcttcttc
    13741 cgcttcgccc ggctcgatca cctccaggtc gcccggtggg ccggctcctt gtgctcgacg
    13801 ttcctcgtcc cgatcctcgc gctcatcgac catcaatcat gtctcctact cgatcgcccg
    13861 cgtgcgactc ccagacggct ggccggagag tcaccccaag atgcttgcca gccgcggaag
    13921 accgcgtccg aacgcggtat acttgcgttt gttatatctt gacgcgccca gcgcgcccag
    13981 ccagaaaagt gatcgctgat gataccgacc tcattgagcg tgcggaactt catgtgctat
    14041 cggtcgaacg tcgagatcga cctgcgcggt g
//
LOCUS       DASUBI010000594         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_193878, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000594 DASUBI010000000
VERSION     DASUBI010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193878"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1344
                     /locus_tag="VFU72_11560"
     CDS             157..1344
                     /locus_tag="VFU72_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424170.1"
                     /translation="MVRRVLCVVMFVLALGLPAAALAEGQTGTITGTVTNGTPGGGGV
                     AGLTVTLGRYSGMDLQQQYTATTDQNGKYAFSDLPITDGEAYVVHVSYQNVDYNGSMI
                     QLTQTPQATSDIKVYETTTDGSKVALTSRGVVIAGADPSSRLIEMLEILSIDNTGDQT
                     YLGANGSVLRIGLPDGAAQIEPQPGFDFGNARMEKGTLITTGAIPPGNSTALLAYGIP
                     YRGTSATLTVPSSMPTGTLQVLVQVNSFTITSLGMNDNGTVNVSEVNYHVLSVDRPIV
                     GDSVTVQVTGLPKAVASGNSAHGPLYAAIAAIAGLLVAAALIFQVLRRRRMASPKLAL
                     AGVPDAPDDERLSLAATLNVLDQEHAAGRIDEADYQARREEVVGQLKAMTLRLRGLEE
                     AED"
     gene            1775..>3227
                     /locus_tag="VFU72_11565"
     CDS             1775..>3227
                     /locus_tag="VFU72_11565"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEU5424171.1"
                     /translation="MQTHLDRDKNDYRSSLYLVPSGGGQPRRFTTADAQDSYPRWSPA
                     GDRVAFLSNRSGKKQLWVIPVAGGEATQLTDLPQPVNAFAWSPDGKTIAFVSKADSAD
                     LEKKDEDEKAKEESDVVRITRLRFRSDGVPGFLDNKPTHIWCVPAEGGEPRQLTNAEQ
                     SDGQPAWAPNGQEIAFVSNRTEEWQSNNASEIWVVPVKGGDARCLVGGSSASFRQPAW
                     SPDGAQLAIVGHQHAGEGGSRNANLWVVPAGGGEPRNLTAGLDRPTTASVSVDTASNA
                     EEAIIWDSRGIYFPVTDQGNVHVYRVTPQSEPALVIGGKRRVLSFSLSGDGSRLAFAA
                     STPVDPCEIYACDASGAQEERLTDVNGEFMRSVEVSEPQEFHVPSHAEDKQPVHGWLM
                     KPIGFQQGQQYPLILEIHGGPHSSYANAYFHEFQLLAAQGYAVVYSNPRGSQGYGEEF
                     AGCTRGTWGESDLPDVLAAVDYAVAQGFVDEGRL"
BASE COUNT          550 a         1077 c         1090 g          510 t
ORIGIN      
        1 catcgagcta ctggaccgcg cctaccaggc gaaggacgtt gccccacgag tcgagctggc
       61 cgccgattga gcggggcggg acggcgcggc cgaggtgcta cacttcgcgc gctggtccgc
      121 gcgtggggac cgacagctgg aggagtgaga gaagcgatgg ttcgccgggt tctgtgcgtc
      181 gtgatgttcg tgctcgcgct ggggctgccg gcggcggcgc tggccgaagg gcagacgggc
      241 accatcacgg gaaccgtcac caacggcacc cccggcggag gcggcgtcgc gggtttgacc
      301 gtcacgcttg ggcgctatag cgggatggat ctgcagcagc agtacaccgc gaccaccgat
      361 cagaacggca agtacgcttt ctccgatctc ccgatcaccg acggcgaggc ctacgtcgtc
      421 catgtcagct accagaacgt ggactacaac ggctcgatga tccagctcac gcagaccccc
      481 caggccacct ccgacatcaa ggtctacgag acgaccaccg acggttcgaa ggtcgcgctg
      541 acctcgcgcg gcgtggtgat cgcgggcgcg gaccccagct cgcgcttgat cgagatgctc
      601 gagattctgt cgatcgacaa taccggcgac cagacgtacc tcggagccaa tggctcggtg
      661 ttgcgcatcg ggttgccgga cggcgcggcg cagatcgagc cgcagccggg atttgacttc
      721 ggcaacgccc gcatggagaa ggggacactg atcacgaccg gggcgatccc tcctgggaat
      781 agcactgcgc tgctggccta cgggattccc taccgcggca ccagcgccac gctgacggtc
      841 ccctcatcca tgccgaccgg gacattgcag gtgctggtgc aggtgaacag ctttaccatc
      901 acctcgctcg gcatgaacga caatggcacc gtcaacgtca gcgaggtcaa ttaccacgtc
      961 ctctcggtcg accggccaat cgtgggcgac agcgtgacgg tgcaggtcac ggggctgccc
     1021 aaggcggtgg ccagcggcaa ctccgctcat ggtccgctct acgcggccat cgcggccatc
     1081 gcggggctgc tggtagctgc cgcgctgata ttccaggtgc ttcgccgtcg ccggatggcg
     1141 tcgccgaagc tggcgctggc gggtgtgccg gacgcgccgg acgacgagcg gctttcgctg
     1201 gcggccacgc tcaacgtgct cgaccaggag cacgccgccg ggcgcatcga cgaggccgac
     1261 taccaggcgc gccgcgaaga ggtcgtcggc cagctcaagg cgatgacgct gcgcctgcgg
     1321 ggcctggagg aagcggaaga ctgacgcgcg ggctcgcgac gactgtgttg gccgccgccc
     1381 cacgatgggt acctccactg cggtcgggat gacagggcca atagggggct gacgacaacg
     1441 gcaatctttc tcgtacgccc gtctctctca ttgtcatcca gagcgcagtc gcaggatctg
     1501 tattccaccc ggcacccaac gccgttgttg gccactaccc ccgcgaactc ttcgtcctgt
     1561 gctactatcc cgccgttgcc ggatggcttc ttgtggggag ccgcaagcga gtaccacccg
     1621 ccgccggtcg gttgcggcgg gacgtcgtga ttgggaggtt ctgatggccg aggccgttgg
     1681 agcgggcaag cgtcgcctga ccgctgatga catttacaat attttgctgg tgaatgacgc
     1741 acagatttct ccggacgcta agcaggtagc ctacgtgcag acccacttgg accgcgacaa
     1801 gaacgactac cgctcctcac tctatctggt gccgtcgggg ggcggtcagc cgcgccgctt
     1861 caccaccgcc gacgcgcagg atagctaccc gcgctggtcg cctgcgggcg atcgcgtggc
     1921 ctttctctcg aatcgctcgg gcaagaagca actctgggtc atccccgtgg ccggcggcga
     1981 agcaacgcaa ctgaccgatc tgccgcagcc ggtcaacgcc ttcgcctggt cacccgacgg
     2041 caagaccatc gcgttcgtgt ccaaagccga ctccgccgac ctggagaaga aggacgagga
     2101 cgagaaggcc aaggaggaga gcgacgtcgt tcgcatcacc cggctgcgct ttcgctccga
     2161 cggcgtgcca ggcttcctcg acaacaagcc aacgcacatc tggtgcgttc ccgccgaggg
     2221 cggcgagccg cggcagttga ccaatgccga gcagtcggat ggtcagcccg cctgggcgcc
     2281 gaacgggcag gagatcgcct tcgtctccaa ccgcaccgag gagtggcagt cgaacaacgc
     2341 cagcgagatc tgggtcgtgc cggtgaaggg cggcgacgcg cgctgcctcg tcggtggatc
     2401 gagcgcgagc ttccgccagc ccgcctggtc gccggacggc gcacagctcg ccatcgtcgg
     2461 ccaccagcac gcgggcgagg gcggttcacg caacgccaac ctctgggtcg tgccggccgg
     2521 cggcggcgag ccgcgcaacc tgaccgccgg gctcgatcgc ccgacgacgg cctcggtgtc
     2581 ggtcgatacc gccagcaacg ccgaggaggc catcatctgg gactcgcggg gcatctattt
     2641 cccggtgacc gaccagggca acgtccacgt ctaccgcgtc acgccccaga gcgagccggc
     2701 gttggtgatc ggcggcaagc gccgcgtcct ctcctttagc ctgagcggcg acggctcgcg
     2761 cctggccttc gcggcctcga ccccggtcga cccctgtgag atctacgcct gcgacgccag
     2821 cggcgcacag gaggagcgct tgaccgacgt caacggcgag ttcatgcgga gcgtcgaggt
     2881 cagcgagccg caggagttcc atgtgccgag ccacgccgag gacaagcagc cggtccacgg
     2941 ctggctcatg aagcccatcg ggtttcagca gggccagcag tatccgttga tcctggagat
     3001 tcacggcggg ccgcactcgt cctacgccaa tgcctacttt cacgagttcc agctcctggc
     3061 ggcgcagggc tatgcggtgg tttattccaa tccacgcggc agccagggct atggcgaaga
     3121 gttcgccggc tgcacgcgcg gcacctgggg cgagagcgat ctgccggacg tgctggcggc
     3181 ggtcgactac gccgttgcgc aggggttcgt ggacgagggg cggctgg
//
LOCUS       DASUBI010000595         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_736488, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000595 DASUBI010000000
VERSION     DASUBI010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736488"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2383)
                     /locus_tag="VFU72_11570"
     CDS             complement(38..2383)
                     /locus_tag="VFU72_11570"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEU5424172.1"
                     /translation="MSATPIQGLGHRVKRKEDPRFIRGKGTYVDDVKLPGMLYLDIVR
                     SPHAHARIKRIDTSRALAMPGVLAVITGKDLEAAGLAWMPTLMSDQQMVLPTDHVVYY
                     AQEIAAVLATDRYLAADAVLEVDVEYEPLEPVVDPFKALEPGAPLVRPDRESKTNHIW
                     HWEAGDQAATEQAFAEAEVVIAQDLYIPRIHVASIETCGCVASYDHATDKLTIWMTSQ
                     APHAHRTVFSLVSGIPETKIRIISPDIGGGFGGKVPVYPGYVLATVATLQTGRPVKWI
                     EDRSENLQADSFARDYHIHAELAAGRDGTMRAMRVRTVADHGAFDAAANPSKFPAGLF
                     SICTGSYAFDAAHVAVDGVYTNKAPGGVAYRCSFRVTEAVHTVERMVDLLADELQMDP
                     AELRLKNFIQPEQFPYKSATGWEYDSGNYPAALSKALAGVGYQDLLREQAEKRARGEL
                     MGIGISTFTEIVGAGPSHTFDILGIKMFDSAEIRVHPIGSAIVRIGVQTQGQGHETTF
                     AQIVAEELGLPVERIVVEHGDTDTAPYGLGTYASRSTPTAGAATAVASRKIREKARLL
                     AAHLLECSPDDLEWQDYAFRVKGAPERSKTMPDLAFAAYTNFPQGLEGGLEAVCYYDP
                     PNLTFPFGAYICVVDIDRGTGQVTIRDFLAIDDCGTVINPMIVEGQIHGGLTMGLAPS
                     LLEEIVYDEHGVMLTGTLMDYLLPTSMETPSWRTDKTVTPSPHHPIGAKGVGESPTVG
                     APPAIANAVVDALSHLGVRHLDIPLTPAKIYAVLQEKGVAE"
     gene            complement(2465..>2876)
                     /locus_tag="VFU72_11575"
     CDS             complement(2465..>2876)
                     /locus_tag="VFU72_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5424173.1"
                     /translation="THIGCDTTSCGACTVHWNGKPVKSCTVFAVQVDGGELRTVEGLE
                     QNGQLHPLQEGFWQEHGLQCGYCTPGMMMTALALLEENPAPSEAEIRLAISGNLCRCT
                     GYVNIVKAIQYAAAKLQPEEAPAPTQPAPAVGDR"
BASE COUNT          417 a          933 c         1037 g          489 t
ORIGIN      
        1 cctcggttcg gagaggaatt cagggaggga cctccgtcta ctccgccact cccttctcct
       61 gcaggaccgc gtagatcttg gctggggtca gcgggatgtc gaggtggcgc acgccgaggt
      121 ggctcagcgc gtcgactacc gcattggcga tggccggcgg cgcgcccacc gtcgggctct
      181 cgcccactcc cttggcgccg atcgggtggt gcggcgaggg ggtcacggtc ttgtccgtgc
      241 gccagctcgg tgtctccatg ctggtcggca gcaggtagtc catcaacgtg ccggtcagca
      301 tgacgccgtg ctcgtcgtag acgatctcct ccagcagcga gggcgccagc cccatggtga
      361 gcccgccgtg gatctggccc tcgacgatca tcggattgat gaccgtgccg cagtcgtcga
      421 tcgccaggaa gtcgcggatc gtgacctgcc cggtgccgcg gtcgatatcg accacgcaga
      481 tgtaggcgcc gaaggggaag gtgaggttcg gcgggtcgta gtagcagacg gcctccaggc
      541 cgccctccag cccctgcggg aagttagtgt aggcggcgaa ggccagatcg ggcatcgtct
      601 tggatcgctc cggcgcgccc ttgacgcgga aggcgtagtc ctgccactcc agatcgtcgg
      661 ggctgcactc gagcaggtgc gccgccagca ggcgtgcctt ctcgcggatc ttgcgcgagg
      721 caaccgcggt cgcggcgccg gcggtcggcg tgctgcggct ggcgtaggtg ccgaggccat
      781 acggcgcggt gtcggtgtcg ccgtgctcga ccacgatccg ctccaccggc aggcccagct
      841 cctcggccac gatctgggcg aaggtggtct cgtggccctg cccctgggtc tgcaccccga
      901 tacgcacgat cgccgaaccg atgggatgca cccggatctc ggcgctgtcg aacatcttga
      961 tgccgaggat gtcgaaggtg tgcgagggac cggcgccgac gatctcggta aaggtgctga
     1021 tgccgatgcc catcagctcg ccgcgagcgc gcttctcggc ctgctcgcgc agcagatcct
     1081 gatagccgac gcccgccagc gccttgctca gcgccgccgg atagttgccg ctgtcgtact
     1141 cccagccggt ggccgactta taggggaact gctccggctg gatgaagttc ttcaggcgca
     1201 gctcggccgg gtccatctgc agctcgtcgg ccagcaggtc gaccatgcgc tcgacggtgt
     1261 gcaccgcctc ggtgacccgg aaggagcagc ggtaggcgac cccgcctggc gccttgttgg
     1321 tgtagacgcc gtcgaccgcc acgtgcgccg cgtcgaacgc gtaggagccg gtgcagatgc
     1381 tgaacagacc cgccgggaac ttggaggggt tggccgccgc gtcgaaggcg ccgtggtcgg
     1441 cgacggtgcg cacgcgcatc gcgcgcatgg ttccgtcgcg cccggcggcc agctcggcgt
     1501 ggatgtgata gtcgcgcgcg aaagagtccg cctgcaagtt ctccgagcgg tcctcgatcc
     1561 acttgaccgg cctgccggtc tggagcgtgg cgaccgtagc caggacatag ccggggtaga
     1621 ccggcacctt gccgccgaag ccgccgccga tgtcgggcga gatgatgcgg atcttggtct
     1681 cggggatgcc cgagacgagc gagaagacgg tgcggtgggc gtgcggcgcc tggctggtca
     1741 tccagatcgt cagcttatcg gtggcgtggt cgtaggaggc gacgcagccg caggtctcga
     1801 tcgaggccac gtggatgcgc gggatataga ggtcctgcgc gatgacgacc tcggcctcgg
     1861 cgaaggcctg ctcggtggcg gcctggtcgc cggcctccca gtgccagatg tggttggtct
     1921 tgctctcgcg gtcgggccgg accagcggcg cgcctggctc gagcgccttg aaggggtcga
     1981 ccaccggctc caacggctcg tactcgacgt cgacctccag cacggcgtcg gcggccagat
     2041 agcggtcggt ggcgagaacc gcggcgatct cctgggcgta gtagacgacg tggtcggtcg
     2101 gcaggaccat ctgctggtcg ctcatcaggg tgggcatcca ggccagtccg gcggcctcca
     2161 gatccttgcc ggtgatgacc gccagcacgc ccggcatggc cagcgcgcgc gaggtatcga
     2221 tgcgcttgat gcgcgcgtgg gcgtgcgggc tgcgcacgat gtccaggtag agcatgccgg
     2281 ggagcttgac gtcgtcgacg taggtgccct tgccacggat gaagcgggga tcctccttgc
     2341 gcttgacccg gtgaccgaga ccctggatcg gtgtggcgct catcgaggtc ttctcctctc
     2401 ggggaaggca gacacggagg tctgtcccca caggcgacgg gcgcgggcag acaacggggc
     2461 ctgcctagcg atctccaact gccggggccg gctgcgtcgg cgcaggggcc tcctccggct
     2521 gcaatttggc cgcggcatat tggatcgcct tgacgatgtt gacgtagccg gtgcagcggc
     2581 agaggttgcc ggagatggcc aggcgaatct cggcctcgct cggcgccggg ttctcctcaa
     2641 ggagcgccag cgcggtcatc atcatgccgg gcgtgcaata gccgcactgg agcccatgct
     2701 cctgccagaa gccctcctgc agcgggtgga gctggccgtt ctgctccaga ccctcgaccg
     2761 tgcgcaactc gccgccgtcg acctgcaccg cgaagacggt gcacgacttg accggcttgc
     2821 cgttccagtg cacggtgcag gcaccgcagc tggtggtgtc gcaaccgatg tgggtg
//
LOCUS       DASUBI010000596         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1255484, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000596 DASUBI010000000
VERSION     DASUBI010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255484"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2520)
                     /locus_tag="VFU72_11580"
     CDS             complement(<1..>2520)
                     /locus_tag="VFU72_11580"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEU5424174.1"
                     /translation="KARTKFVCQSCGFESPQTYGRCPGCGEWNTLVETMEAKPTARDR
                     RPVAPLAARPTRLLDVASAEHARHVVPMEEFNRVLGGGLVPGSLVLVGGDPGIGKCVT
                     GDTRVLDPATGAYLPISVWANEEHPVLAVDDESLRLDQVAVSAFHERGIRPVVDVVTG
                     MGRTLRCTPDHPVLTPEGWRPVGQLAPGDRVAAPRALPYFGSLDMPEHQVKLIAYILS
                     DGSAHSATTITTALAEVEEDIRQIAAAFQMRVVRYSKPHTKAVQLRLTSDKEQRANAR
                     QEVAGVLRSARARAGQTWQSWSDRAEVSYDMLNQWRRGTCVPSIDELQRLADAVDLPL
                     AALAPTARDLADMTTPIARFLTYVGLRYSRAADKAVPACIFQLPREQLALFLRTLFTC
                     DGSVFINQQDQPGVSYSTISHQLATDVQHLLLRFGLIARLRTKRSRVSGRAYTAYELQ
                     LLGVANVQRFLAEIGILGRAAARARIAELEPASLPSTRVDTIPTGTQFWAMLDTARRA
                     AGFDDYRVLSRHAGATIRNRRHDRPLARATVVRLAEVLDEPRLRSLAFGDIYWDEIES
                     ISLAGEAPVFDLTVPGKANFVANDLVIHNSTLLLQTASQLAERIGTVLYVSAEESAQQ
                     VRLRADRLGLRPESLFVLSETNLDSMLVAAEQLDPGLLIVDSIQTVYLDDLTSAAGSV
                     SQVRECTSRLMQFAKPRGLPVLIVGHVTKEGAIAGPRVLEHMVDAVLYLEGDRYHQYR
                     VLRAVKNRFGSTDEVGVFEMADIGMREVRNPSEAFLMERDGNAAGSAVAVTIEGTRPI
                     LLEVQGLTTASSFGQPRRTANGFDSNRLQMLVAVLSKRVGLG"
BASE COUNT          464 a          848 c          785 g          423 t
ORIGIN      
        1 ccccagaccg acgcgtttgc tgagcaccgc caccagcatc tgcagccggt tggagtcgaa
       61 gccgttggcc gtgcggcgcg gctggccaaa gctgctggcg gtcgtcagcc cctgcacctc
      121 cagcagaatc ggccgcgtgc cctcgatcgt caccgccacc gccgagccag ccgcgttgcc
      181 gtcgcgctcc atcaggaacg cttcggaggg attacgcacc tcgcgcatgc cgatgtcggc
      241 catctcgaag acgccgacct cgtcggtcga gccaaagcga ttcttgaccg cgcgcagcac
      301 gcggtactgg tggtagcggt cgccctccag gtagagcacg gcgtcgacca tgtgctccag
      361 cacgcgcggc cccgcgatcg cgccctcctt ggtgacgtga ccgacgatca gcaccggcag
      421 accgcgcggc ttggcgaact gcatcaggcg cgaggtgcac tcgcgcacct ggctcacgct
      481 accggcggcg gaggtcaggt cgtcgagata aacggtctga atcgagtcga cgatcagcag
      541 ccccgggtcg agctgctcgg ccgctaccag catgctgtcg aggttggtct ccgacaagac
      601 gaaaagcgac tccggccgca accccagccg gtcggcccgc agccgcacct gctgcgccga
      661 ctcctcggcc gagacataga gcaccgtccc gatgcgttcc gctaattgtg aggctgtctg
      721 caacagcagt gtcgaattgt ggattaccag gtcattcgcg acaaaattcg ccttgcccgg
      781 taccgtcaag tcaaagaccg gtgcctcgcc ggctaggctg attgactcaa tctcatccca
      841 atagatgtcg ccgaacgcca ggcttcgtag gcgaggttcg tcaagcacct cagccagccg
      901 cacgaccgta gcccgagcca gcggccggtc gtgccgacga tttcggatcg tggctccagc
      961 atgacgcgag agcacacgat aatcgtcaaa gccagcggcc cgccgagcag tatcgagcat
     1021 cgcccaaaac tgcgttccgg tcgggatcgt atcaacgcgc gtggatggca aacttgccgg
     1081 ctccagctca gcaatacgcg cccgagccgc cgcgcggccg agaataccaa tctctgcaag
     1141 gaatcgttgc acattggcaa cacctagtag ctgcagctcg tatgccgtgt aagcccgccc
     1201 gctgacgcgg ctccgcttgg ttcgcaaccg cgcgatcaaa ccaaatcgga ggaggaggtg
     1261 ctgcacatca gtagccaact gatggctgat ggtgctgtag ctgacgccgg gctgatcctg
     1321 ttggttgatg aagactgatc cgtcacaggt gaagagggtt cgcaaaaaga gcgcgagttg
     1381 ctcgcgaggc agttggaaga tgcatgcggg cacagccttg tctgcggctc gcgagtagcg
     1441 aaggccgacg taggtcagga aacgagcgat cggcgtcgtc atgtcggcca gatcacgtgc
     1501 cgtcggtgcg agtgcggcga gtggcagatc gaccgcatcg gcaagccgtt gcagttcatc
     1561 gatgctcggc acacaggtac cgcgacgcca ctggttcaac atgtcgtaac tcacctccgc
     1621 acgatcagac cagctctgcc aggtttgccc cgctctagcg cgcgcgcttc tcagcacgcc
     1681 cgccacctct tgccgcgcgt tcgcccgttg ctccttgtcg ctggtcaagc ggagttgaac
     1741 cgccttggtg tgcggcttcg agtagcgaac cacgcgcatt tggaacgcag ctgcgatctg
     1801 ccggatatcc tcctctactt cagccagcgc ggtcgtgatc gtggtcgctg aatgggcgga
     1861 tccatccgag aggatgtagg cgatcagctt cacctggtgc tcgggcatgt cgagtgaccc
     1921 gaagtagggc aacgcacgcg gagcggcgac acggtcacct ggcgcgagtt gccccaccgg
     1981 acgccagcct tccggggtga ggacgggatg atcaggtgtg cagcggagtg tgcgtcccat
     2041 gccggtgacg acatccacaa ccggccgtat accccgctcg tgaaacgccg ataccgccac
     2101 ctgatcaagc cggagcgact cgtcatcgac agcgagtacc ggatgctcct cgttcgccca
     2161 gaccgaaatc gggagatagg ctcccgtcgc cggatccagg acgcgggtat cccccgtcac
     2221 gcatttgccg atcccaggat cgccgcccac cagtaccagc gagcccggca ccaggccgcc
     2281 gccgaggacg cggttgaact cctccatcgg caccacgtga cgcgcgtgct cggcgctggc
     2341 gacgtccagc agccgcgtcg ggcgcgccgc gagcggcgcg acgggacgcc ggtcgcgcgc
     2401 cgtcggcttg gcctccatcg tctcgaccag ggtgttccac tccccacaac ccgggcagcg
     2461 cccgtaggtc tgcgggctct cgaatccgca gctctgacag acgaacttgg tgcgcgcctt
//
LOCUS       DASUBI010000597         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1010419, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000597 DASUBI010000000
VERSION     DASUBI010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010419"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFU72_11585"
     CDS             <1..685
                     /locus_tag="VFU72_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5424175.1"
                     /translation="LAEQAAESLDRVYRRRGVVRLELEGGTDGQPRLGALITADSERL
                     VYDGVVLALPPEALARVLGEPERLGLEGLDRFQSAPIVDVHLWYDAGHLGFGFAALVD
                     SPVQWVFEKAPGYLCCSLSAAEEHVLRPEAELIELCHRELAALLPALASRRPLRGAAT
                     REREATFIPAPGLRRPGPRTACPQVVIAGSWTDTGWPATMESAVRSGRAAAKTLAANL
                     AGVGAGGDG"
     gene            678..2531
                     /gene="shc"
                     /locus_tag="VFU72_11590"
     CDS             678..2531
                     /gene="shc"
                     /locus_tag="VFU72_11590"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU5424176.1"
                     /translation="MAEALGQAVQWLRERQDPAGWWRGELETNVTMTAEHILLLRFLG
                     LPLEPIRDGALVHLLSNQREDGSWALYYAGPADLSTTIEAYVALKVLGVDPASAPMTR
                     ALRVILQLGGLARARVFTKIWLALFGQYPWRGVPSMPPELYYLPSNLPFNLYDFACWA
                     RGTIAPLLIVLSRRPCRPLGCELAELVTPGSEPLLERVPGGGLFWWADRLLKRYDSLP
                     RQPGRGRARRRLVDWIVERQEADGGWGGIQPPWVYSLIALNLEGMALDHPVMRRGLAG
                     LTGFAWEDEGGWRLEACQSPVWDTAWAVIALRRAGVPRHDPQLRAAVRWLLDEQITRG
                     GDWQVRVPEPVACGGWAFEFANDTYPDIDDTAVVAVALLDAVDEDDEDAVQEAVGRAV
                     RWVLAMRSRNGAWAAFDRDNTSRMPYRLPFADFGALTDPPTEDVTAHVLELLARTGYG
                     PTEPVVAGGLAYLRRAQRPDGSWWGRWGVNHIYGTWCVVAALAALYGPGARLPARAGR
                     ALDWLLARQNPDGGWGETCHSYVDPSFAGVGESTPSQTAWAALALQLAGGGHGAEPAC
                     RRGLDFLRVRQQGGTWDEPQYTGTGFPRDFYINYHLYRHVFPLMALAGDRV"
     gene            2574..3350
                     /locus_tag="VFU72_11595"
     CDS             2574..3350
                     /locus_tag="VFU72_11595"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU5424177.1"
                     /translation="MAMGNEPTYHLAGRVALVTGAASGLGRAAACAFARGGARVVVAD
                     IEPDGGAETVALITGAGGEASFVPVDVSREAEVEAMVQYALDTYGRLDYAYNNAGIMT
                     GGIPLEEYTEDVWDEIMAVNLKGVWLCLKYELPVMRAQGGGAIVNASSVTGLHGEAGR
                     AAYAASKHGVIGLTRVAALEGAPAGIRVNAVCPGWVRTPPTEFVLSLNPGLEDDLRRV
                     APLGRLGTAEDVAEAVVWLCSDAAGYITGQSLALEGGMLA"
     gene            complement(3361..3669)
                     /locus_tag="VFU72_11600"
     CDS             complement(3361..3669)
                     /locus_tag="VFU72_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5424178.1"
                     /translation="MHANVSLWKAKSPDSLEKLLSKAEAELVPKLQSQPGFLSFQVVR
                     ADGDTLILIHTWESKDKADVGLRRLGPWLLTNAATKLSLISRHSGELVLSSLNTEVAG
                     "
     gene            complement(3719..>4490)
                     /locus_tag="VFU72_11605"
     CDS             complement(3719..>4490)
                     /locus_tag="VFU72_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424179.1"
                     /translation="FRGPEFPPLGNWECWTTAAAIGAATSRVEIGTLVSSTSFRPPAV
                     LAAMANTIDDLTDGRLILGLGAGDAEKEHLAMGIPFEQRVGRFEEALTILRALFREGE
                     SSVDGRFYQTRACQLLPAGPRAGGPPFLIGALAQSPRMLRLTAQYADIWNVTLTFGRS
                     RPDVVPPLRTAVDAACRKYGRDPATLARSAAVLVDCTGFGWYPEGLEPLVGSPEELAE
                     SFRAFAREGISHLQVATYPATIEGIEELAPVLEILDGD"
BASE COUNT          599 a         1539 c         1677 g          675 t
ORIGIN      
        1 gctggccgag caggcggcag agtcgctcga ccgggtctac cgccgccgcg gcgtggtcag
       61 gctggagctg gagggcggga cggacggcca gccgcgcctg ggcgcgctta tcaccgccga
      121 cagcgagcgg ctcgtgtacg acggcgtggt gctggccctg ccgccggagg cgctggcgcg
      181 ggtgctcggc gagccggagc gcttggggtt ggaggggctc gaccgcttcc agagcgcgcc
      241 gatcgtcgac gtgcacctgt ggtacgacgc cggacacctc ggtttcggct tcgccgcgct
      301 ggtcgactcg ccggtgcagt gggtcttcga gaaggcgccc ggctacctct gctgcagcct
      361 gagcgcggcc gaggagcacg tgctgcggcc cgaggcggag ctgatcgagc tctgccaccg
      421 cgagctggcc gcgctgctac cggcgctggc cagtcgacgt ccgctgcgcg gcgcggccac
      481 ccgcgagcgc gaggcgacct tcatcccggc gccggggctg cgccggccgg gtccgcgcac
      541 ggcttgtcca caggtagtca tcgctggatc gtggaccgac accggctggc cggcgacgat
      601 ggagtcggcg gtgcgcagcg ggcgcgcggc ggcgaagaca ctggcggcga acctggcggg
      661 agtaggggcg ggcggcgatg gctgaggcgc tggggcaggc cgtgcagtgg ctgcgcgagc
      721 ggcaggaccc ggcgggctgg tggcgcggcg agctggagac gaacgtcacc atgaccgccg
      781 agcacatcct gctgctgcgc ttcctgggcc taccgctgga gccgatccgc gacggcgcgc
      841 tggtccacct cttgagcaac cagcgcgagg atggctcctg ggcgctctac tacgccggcc
      901 cggccgatct gagcaccact atcgaggcct acgtcgcgct caaggtgctg ggggtcgatc
      961 cggccagcgc gccgatgacg cgggcgttgc gcgtcatcct ccagctgggt ggtctggcgc
     1021 gggcgcgcgt cttcaccaag atctggctgg cgctcttcgg ccagtacccc tggcgcggtg
     1081 tgcccagcat gccgcccgag ctgtactacc tgccatcgaa cctccccttc aacctctacg
     1141 acttcgcctg ctgggcgcgc ggcaccatcg cgcccctcct gatcgtgctc tcgcgccgtc
     1201 cctgccgccc gctcggctgc gagctggccg agctggtgac gcccggcagc gagccgctcc
     1261 tggagcgggt tccgggcggc ggcctcttct ggtgggccga ccgcctgctg aagcgctacg
     1321 acagcctgcc gcgccagccc ggacgcgggc gcgctcgtcg ccgcctggtc gattggatcg
     1381 tggagcggca ggaggccgac ggcggctggg gcggcatcca accgccctgg gtctactcac
     1441 tgatcgcgct caacctggag gggatggcgc tcgaccaccc ggtgatgcgc cgtggcctgg
     1501 ccgggctgac tggcttcgct tgggaggacg agggcggctg gcggctggag gcctgccagt
     1561 caccggtctg ggacaccgcc tgggccgtca tcgccctgcg gcgagcaggc gtgccgcgcc
     1621 acgatccgca gttgcgcgcg gcggtgcgct ggctgctcga cgagcagatc acgcgcggcg
     1681 gcgattggca ggtgcgcgtg ccggagccgg tcgcgtgcgg cggctgggcc ttcgagttcg
     1741 ccaacgatac gtaccccgac atcgacgaca ccgccgtggt ggccgtggcc ctgctcgatg
     1801 ccgtcgacga ggacgacgag gatgcggtgc aggaggccgt cgggcgggcg gtgcgctggg
     1861 tgctcgcgat gcgctcgcga aacggcgcct gggcggcctt cgaccgcgac aataccagcc
     1921 ggatgcccta tcggctcccc ttcgccgact tcggcgcgct gaccgatccg cccacggaag
     1981 acgtgaccgc gcacgtgctg gagctgctgg cgcgcaccgg ctacggcccc acagagccgg
     2041 tggtggccgg cgggctggcg tacctgcggc gcgcgcaacg gcccgacggc tcctggtggg
     2101 gccgctgggg cgtcaaccac atctacggca cctggtgcgt cgtggcggcg ctggcggcgc
     2161 tctacgggcc gggcgcgcgg ctccctgccc gcgccgggcg cgcgctcgac tggttgctgg
     2221 cgcgccagaa cccggatggt ggctgggggg agacctgcca ctcctacgtc gacccctcct
     2281 tcgcgggagt gggcgagagc acgccgtcac agaccgcctg ggccgcgctg gcgctccaac
     2341 tggccggggg tggccacggc gccgagcccg cctgccggcg cggcctcgac ttcctgcgcg
     2401 tgcgccagca gggcggaacc tgggacgagc cgcagtacac cggcaccggc ttcccgcgcg
     2461 acttctacat caactaccac ctctaccggc acgtcttccc gctcatggcg ctggctggcg
     2521 acagggtatg attggcgctg gcattcaccg gtggcatgac gagaaacctg gagatggcaa
     2581 tggggaacga gccgacgtat cacctcgccg gccgcgtggc gctggtgacg ggcgccgcgt
     2641 cgggcctggg tcgcgccgcc gcgtgcgcgt tcgcccgcgg cggggcgcgc gtcgtggtgg
     2701 cggatatcga gccggatggg ggtgcggaga ccgtcgcgct gatcaccggg gccgggggtg
     2761 aggcgagctt cgtgccggtg gatgtcagcc gcgaggccga ggtcgaggcg atggtccaat
     2821 acgcgctcga cacctatggc cggctcgact acgcctacaa caacgccggc atcatgaccg
     2881 gcggcattcc gctggaagaa tataccgagg atgtctggga tgagatcatg gccgtgaacc
     2941 tgaagggcgt ctggctctgc ctcaagtatg agctgccggt gatgcgcgcc caggggggcg
     3001 gcgcgatcgt caacgcgtcc tcggtcacgg ggctccacgg cgaagccggc cgggccgcct
     3061 acgcggcgag caagcacggc gtgattgggc tgacccgtgt cgcggcgctg gagggcgcgc
     3121 cggccggcat tcgcgtcaac gccgtctgcc ctggctgggt gcgcacgccg ccgacggagt
     3181 tcgtgctctc gctgaatccc ggcctcgagg acgacctgag gcgtgtggcg ccgcttgggc
     3241 gcctcggcac ggcggaggat gtggccgagg cggtcgtctg gctctgctct gacgccgccg
     3301 ggtacattac cggacagtcg ctggcgctcg aaggcggcat gctcgcctaa tgccgggacc
     3361 ctacccggcg acctcggtgt tgaggcttga gaggaccagt tccccgctgt ggcggctgat
     3421 cagactcagc ttggtggcgg cgttggtcag cagccagggc cccaggcgcc gcaggccgac
     3481 atccgccttg tcctttgatt cccaggtgtg gatgaggatg agcgtgtcgc catccgcccg
     3541 gaccacctga aaactgagaa atcccggctg gctctgcagc ttgggcacga gttcggcctc
     3601 cgccttgctc agcagtttct ccagactgtc cggcgacttc gccttccaca gggatacgtt
     3661 cgcgtgcatg ttgaccgtgc ctctcacttc attcgcccat cggctacgcg ccctgggctc
     3721 agtcgccgtc gaggatctcc agcaccgggg cgagctcctc gatcccctcg atcgtcgccg
     3781 gataggtagc cacctgaaga tgggagatgc cctctcgtgc gaaggcgcgg aacgactcgg
     3841 cgagttcctc gggcgacccc accagcggtt ccagcccttc cggataccag ccgaacccgg
     3901 tgcagtcgac cagcaccgcc gcgctgcggg ccagggtggc cgggtcgcgc ccgtacttgc
     3961 ggcaggcggc gtcgaccgcg gtacgcagcg gtgggacgac gtccgggcgg ctgcgcccaa
     4021 aggtcagggt gacgttccaa atgtcggcgt attgggcggt caggcggagc atgcgcggcg
     4081 attgcgccaa ggcgccgatc aggaaaggcg ggccgccggc gcgcgggcca gccggcaaga
     4141 gctggcacgc gcgcgtctgg tagaagcggc cgtcgacgct gctctcacct tcacggaaga
     4201 gggcgcgcaa gatcgtgagg gcttcctcga agcggccgac gcgctgctcg aaggggatgc
     4261 ccatcgccag gtgctccttc tcggcgtcac cggcgcccag accgaggatg agccgcccgt
     4321 cggtcagatc gtcgatggtg ttggccatgg cggccagcac cgcgggcggc cgaaaggagg
     4381 tgctgctgac cagggtgccg atctcgacgc gcgacgtcgc cgcgccgatc gccgcggccg
     4441 tcgtccagca ctcccagttg ccgagcggag gaaactccgg gccgcgaaag
//
LOCUS       DASUBI010000598         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_588513, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000598 DASUBI010000000
VERSION     DASUBI010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588513"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1929
                     /locus_tag="VFU72_11610"
     CDS             127..1929
                     /locus_tag="VFU72_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5424180.1"
                     /translation="MPTPLMTPPNPAAVVAAAASAGADFLHPGYGFLSERAALARACA
                     AAGIVFVGPSPETLELCGDKIATRQAAARVGVPVPAASPPLGAESEEWPALAAAVGYP
                     LLVKAAGGGGGASLRQVDRPEDLAAAVASSRREAEAAGAGGVLYLERLLPQARHIEVQ
                     VAGDGQRAIAIGERECSLQRRHQKVIEEAPAANLPEPDRGRLFAYAVAVAEAVGLRNL
                     ATIEFLYGQDGTIAFIEVNPRLQVEHPVTELVSGLDLVALQLDLAAGGQLPASLPAPR
                     GHAVEARLYAEDPAREFLPSPGPLAVFAPPELPGLRVDAGYQRGDRVPEHYDPLLAKL
                     VGSGATRVEALDRLRDALARTGVAGVATNRAWLLALLDDTRVREGRYDTLTAEEIPAP
                     SPAGGPEPLALGALAAAMLDRPPSNDPWDRLGPFRLDGAAELAFHGPGWERVAAVERQ
                     DGAWQLAEDGRRASLAWWHGPDGLWTVRLGELAGRRAVARHLDGTIEGAGARGRWLAR
                     PGRQAASQGRTARRAEGTLRAPLPGKVLRVEIAPGERVSEGQPLVILSAMKLEVVLRA
                     PFDGTVRAVHGRPEEQVEAGAVLVELEPEAEDES"
     gene            1926..>2693
                     /locus_tag="VFU72_11615"
     CDS             1926..>2693
                     /locus_tag="VFU72_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5424181.1"
                     /translation="MSLVERLPRRVTVVEVGPRDGLQNEPQIVPTEAKIAFVEALAAA
                     GLPVVEATSFVSPKAVPQLADAEAVMRGITRRPGTRYPVLVPNERGLERALAAGVDAI
                     ALFTAASEEFSRHNVRASIDETFDRFRPVAEQARAAGLWLRGYVSTAIDCPYSGPVAP
                     EQAVAVAERLFELGCQEVALADTIGTATPRAVDRLLARTAARLGLERIGLHFHDTGGL
                     ALANVMIGLDYGVRVFDAAAGGLGGCPFAPGAPGNLAT"
BASE COUNT          329 a          995 c         1023 g          346 t
ORIGIN      
        1 ccgatgacgc gcgcggcgta gccggcgcgg tcgggctcgc cgcagaccag caccggctcc
       61 agcccgcgca ggcggcaggt ggcggcgatg cgcgccgcga cctcgccccg gttggcgatg
      121 gcgaccgtgc ccacgccgct catgacacca cccaatccgg cggccgtggt ggcggctgcg
      181 gccagcgccg gcgccgactt cctccacccc ggctacggtt tccttagcga gcgcgccgcg
      241 ctggcccgcg cctgcgcggc ggccggtatc gtcttcgtcg gcccctcgcc ggagacactg
      301 gagctgtgcg gcgacaagat cgcgacgcgg caagcggctg cacgcgtggg cgtgccggtg
      361 ccggcggcaa gcccgccgct cggcgccgag tcggaggagt ggccggcgct ggcggctgcg
      421 gtcggctatc cactgctggt gaaggcggcc ggtggcggcg gcggggccag cttgcgccag
      481 gtcgaccggc ctgaagacct ggccgcggcg gtcgcctcct cgcgccgcga ggccgaggcg
      541 gccggcgccg gcggtgtcct ctaccttgaa cgcctgctgc cgcaggcccg ccacatcgag
      601 gtgcaggtgg ccggtgacgg ccagcgcgcc atcgccatcg gcgagcgcga gtgctcgctg
      661 cagcgccgcc accagaaggt gatcgaggag gcgcccgccg cgaacctgcc cgagcccgac
      721 cgcggccgcc tcttcgccta cgccgtggcg gtggccgagg cggtcgggct gcgcaatctc
      781 gcgacgatcg agttcctcta cggccaggat ggcaccatcg ccttcatcga ggtcaacccg
      841 cgtctccagg tcgagcatcc ggtgaccgag ctggtcagcg ggctcgactt ggtggcccta
      901 cagctcgatc tggccgccgg gggacaactc cccgcttcgc tgccggcgcc gcgcggccac
      961 gccgtcgaag cgcggctcta cgccgaggat ccggcgcgcg agttcctccc ctcacccggc
     1021 ccgctggcgg tcttcgcgcc gcccgagctg cccggcctgc gcgtcgacgc cggctaccag
     1081 cgtggcgacc gcgtgcccga gcattacgat cctctgctgg ccaagctggt cggcagcggc
     1141 gcgacgcgcg tcgaggcgct cgaccggctg cgcgatgccc tggcgcgcac cggcgtggcc
     1201 ggggtggcga ccaaccgcgc ctggctgctg gccctgctcg acgacactcg cgtgcgggag
     1261 ggccgctacg atacgctgac cgccgaggag atcccggcgc cgagcccggc cggtggaccc
     1321 gaacccctgg cgctgggcgc gctggccgcc gccatgctcg atcgtccgcc gagcaacgat
     1381 ccctgggacc ggctcggccc cttccggctc gacggcgccg cggaactggc attccatggt
     1441 cccggctggg agcgcgtggc ggcggtcgag cgccaggacg gcgcttggca attggccgag
     1501 gacggccggc gcgcgtcact ggcctggtgg cacggcccgg acgggctctg gacggtgcgg
     1561 ctgggcgagc tggcgggacg gcgcgccgtg gcgcgccacc tcgacgggac catcgagggg
     1621 gccggggcgc gcgggcgctg gctggcgcgg ccggggcggc aggcggcgag ccagggacgc
     1681 accgcgcgcc gggccgaggg taccctgcgc gcgccgctgc cgggcaaggt gctgcgagtc
     1741 gaaatcgcac ccggcgaacg cgtcagcgag ggccagccgc tggtgatcct cagcgcgatg
     1801 aagctcgagg tcgtgctgcg ggcaccgttc gacggtaccg tacgcgccgt ccacggccgg
     1861 ccggaggagc aggtcgaagc cggggcggtc ctggtcgaat tggagccgga agcggaggac
     1921 gagtcatgag cctggtcgag cgcctgccgc gccgggtgac ggtggtcgag gtcggcccgc
     1981 gcgacgggct gcagaacgag ccgcagatcg tgccgaccga ggccaagatc gccttcgtcg
     2041 aggcgctggc cgcggccggg ctgccggtgg tcgaggcgac cagcttcgtc agcccgaagg
     2101 cggtgccgca actagccgac gccgaggcgg tcatgcgcgg catcacgcgc aggcctggca
     2161 cccgctaccc cgtgctggtg cccaacgagc gcggcctgga gcgggcgctg gcggccggcg
     2221 tcgacgccat cgcactcttc accgccgctt ccgaggagtt cagccggcac aacgtgcgcg
     2281 ccagcatcga cgagaccttc gaccgcttcc gcccggtggc tgagcaggcc cgcgccgccg
     2341 ggctctggct ccggggatat gtctcgaccg ccatcgactg cccctacagc gggccggtcg
     2401 cgcccgaaca ggccgtggcc gtggccgagc ggttatttga actgggttgc caggaggtcg
     2461 cgctggccga caccatcggc accgctaccc cgcgcgccgt cgatcgcctg ctggcgcgaa
     2521 ccgcggcgcg actcggactg gagcggatcg ggctccattt tcatgacacc ggcgggctgg
     2581 cgctggccaa cgtgatgatc gggctcgact acggcgtgcg cgtcttcgac gccgcggccg
     2641 gcggtctggg cggctgtccc ttcgctcccg gcgcacctgg caacctggcc acc
//
LOCUS       DASUBI010000599         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_57201, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000599 DASUBI010000000
VERSION     DASUBI010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57201"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFU72_11620"
     CDS             <1..471
                     /locus_tag="VFU72_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424182.1"
                     /translation="EACPKGVAPMSQIMRLRRIAGAEYGIDDRNNGHRHEMAFVRNIE
                     KNGLLHEADLLPDSYGGKFHPRAVPELMSSLPVILKALRRGKVTPAGALLHKHKAPKE
                     VKRIFRDVHDREARLELNLYVTGYEDDDGGETTGEQAHADAAGSHMPEERSPNQ"
     gene            468..1376
                     /locus_tag="VFU72_11625"
     CDS             468..1376
                     /locus_tag="VFU72_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEU5424183.1"
                     /translation="MKVAYWPGCVSRGFTPELHGSMAKVAPLLDIELVELDRAACCGA
                     GVIAEHNQELADTLNARTFALAQNEIAGGADLMMNICSTCQGAQNECQERLDANTEYR
                     DHVNETLSAEGLTYRRGIVNKHFLWLMVEEIGLDAIRAKVKRPLENLRVGPFYGCYIV
                     RPTDRLGIDRAHPRDTYLHLLIEALGGTVVDYAGQYKCCGFPIITMNKEASLKQAGRH
                     IGDAIDADADCLVTPCPLCHLNLDLQQPLASGVVGRPLGLPILHLPQLVGLAFGLDPK
                     ELGMGKHVVKPTSVIDWSTSVVASAA"
     gene            1503..3233
                     /locus_tag="VFU72_11630"
     CDS             1503..3233
                     /locus_tag="VFU72_11630"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEU5424184.1"
                     /translation="MAKRGGRVHVATTRRHYKDKEYVTTLLRRSYREGGKVRNETLAN
                     LSHLPPAAIDALRAVLAGRALVDADEHVRVARSLPHGHVAAVLGVLRDLDLERVLGRE
                     RCRERDLVVAMVCQRLIAPGSKLSATRRFGQTTLAEELALGEVTEAELLGSLDWLGER
                     QERIEATLARRHLAPGGFVLYDLSSSYVEGRACPLAKLGYSRDATKGKLQITYGLICS
                     PDGRPVAVRVHDGNTQDQQTLPSAVAAVTERFGIERVVFVGDRGMITSAHADALKAQG
                     VGLLSALKAPQIRALVDAGELQLSLFDQTNLAEITSQRFPDERLIVCRNPAVAAERAR
                     KRAELLAATETELDKVAAMVTGPRGSLRSADAGRIGERVGRVANKHKMAKHFQLDITD
                     GHFAYQRKHPQIQAEAALDGLYVLRSTVAQPDLASAAAVRAYKQLKLAERAFRTLKDT
                     LEIRPIYHHLEARVRAHVFLCMLAYYLSFELRTRLTPLLFDDETPLAPTDPVAPAIRS
                     PAALTKAGSARTRDGHPAHTLPDLLSELGTLCRNHLRIGTSHHTFTQLTEPTTLQARA
                     LELLDIKPAK"
BASE COUNT          566 a         1265 c         1124 g          489 t
ORIGIN      
        1 gaggcctgcc cgaagggcgt ggcgccgatg agccagatca tgcggctgcg gcggatcgcc
       61 ggcgccgagt acggcatcga cgaccgcaac aacggccacc ggcacgagat ggccttcgtg
      121 cgcaacatcg agaagaacgg cctgctgcac gaggccgacc tgctgcccga ctcctacggc
      181 ggcaagttcc acccgcgcgc cgtgccggag ctgatgagct cgctgccggt gatcctcaag
      241 gcgctgcggc gcggcaaggt gacgccggcg ggcgcgctgc tgcacaagca caaggcgccc
      301 aaggaggtca agcggatctt ccgcgacgtc cacgaccgcg aggcgcggct ggagctcaac
      361 ctctacgtca ccggctacga ggacgacgac gggggcgaga cgaccgggga gcaggcgcac
      421 gccgacgccg ccggcagcca catgccggaa gagaggagcc ccaaccagtg aaggtcgcgt
      481 actggcccgg ctgcgtgagc cgcggcttca ccccggagct gcacggctcg atggccaagg
      541 tcgcgccgct gctggacatc gagctcgtgg agctcgaccg cgccgcctgc tgcggcgccg
      601 gcgtcatcgc cgagcacaac caggagctgg cggacaccct gaacgcgcgc acgttcgcgc
      661 tcgcccagaa cgagatcgcg ggcggcgcgg acctgatgat gaacatctgc tccacctgcc
      721 agggcgcgca gaacgagtgc caggagcgcc tggacgcgaa caccgagtac cgcgaccacg
      781 tcaatgagac gctgtccgcc gaggggctca cctaccggcg cgggatcgtc aacaagcact
      841 tcctctggct gatggtcgag gagatcggcc tggacgcgat ccgcgcgaag gtcaagcggc
      901 cgctggagaa cctgcgcgtc ggcccgttct acggctgcta catcgtccgc ccgaccgacc
      961 ggctggggat cgaccgcgcg cacccgcgcg acacctacct gcacctgttg atcgaggcgc
     1021 tgggcggcac ggtggtcgac tacgcgggcc agtacaagtg ctgcggcttc ccgatcatca
     1081 ccatgaacaa ggaggcctcg ctcaagcagg cgggccgcca catcggtgac gcgatcgacg
     1141 ccgacgcgga ctgcctggtc acgccgtgtc cgctctgcca cctcaacctc gacctccagc
     1201 agcccctggc ctccggggtc gtcgggcgcc cgctcgggct gccgatcctg caccttcccc
     1261 agctcgtcgg cctggccttc ggcctggacc cgaaggagct gggcatgggc aagcacgtgg
     1321 tcaagccgac gtcggtcatc gactggtcga cgtccgtggt ggcctcggcc gcctagacgg
     1381 aagttttcgt ggtcgttgag gcccgattgc tcggctggcg ggcggtcggg tctcttcttt
     1441 cccggtctgt gtagacacta aaatggttgc ggtgcgccgt tccgtgtggg atcatgctgg
     1501 tcatggcgaa gcgtggcggt cgggtgcatg tggcgacgac tcgtcgtcac tacaaggaca
     1561 aggagtacgt gaccacgctg ctgcggcgct cctatcgcga gggcggcaag gtgcgcaacg
     1621 agacgctggc caacctctcg catctgccgc cggcggcgat cgacgcgttg cgcgcggtgc
     1681 tggccggccg cgcgctggtc gacgccgacg agcacgtgcg cgtggcgcgc tcgttgccgc
     1741 acgggcacgt cgccgcggtg ttgggcgtgc tgcgcgatct tgatctggag cgcgtgctcg
     1801 gccgtgagcg ctgccgcgag cgcgatctgg tcgtcgccat ggtctgtcag cggctgatcg
     1861 ctcccggctc caagctgtcg gccacacgcc gcttcgggca gaccacgctg gccgaggaac
     1921 tcgcgctggg cgaggtcacc gaggccgagc tcttgggctc gctggactgg ctcggcgagc
     1981 gccaggagcg gatcgaggca acgctcgcgc gccgccattt ggcgcccggc ggcttcgtgc
     2041 tctacgacct ttcctccagc tacgtggagg gccgcgcgtg cccgctggcc aagctgggct
     2101 actcgcgcga tgccaccaag ggcaagctgc agatcaccta cgggctgatc tgctcgcccg
     2161 acgggcggcc ggtggcggtg cgcgtgcacg acggcaacac gcaggatcag cagacgctgc
     2221 cctcagcggt cgccgccgtg accgagcgtt tcgggatcga gcgggtggtg ttcgtcggcg
     2281 atcgcggcat gatcaccagc gcgcacgccg acgccctcaa ggcccaaggc gtcggcctct
     2341 tgagcgcgct caaggcgccg cagatccgcg cgctagtcga cgccggcgag ctgcagctct
     2401 cgctctttga ccagaccaac ctggccgaga tcaccagcca gcggttcccc gacgagcgcc
     2461 tgatcgtctg ccgcaacccg gccgtcgccg ccgagcgcgc gcgcaagcgc gccgagctgc
     2521 tggccgccac cgagaccgag ctcgacaagg tcgccgcgat ggtcacagga ccgcgcggca
     2581 gcctgcgcag cgccgacgcc ggccgcatcg gcgagcgcgt cggccgcgtc gccaacaaac
     2641 acaagatggc caagcacttc cagctcgaca tcaccgacgg ccacttcgcc taccaacgca
     2701 aacaccccca gatccaagcc gaggccgccc tcgacggcct ctacgtgctg cgcagcaccg
     2761 tcgcccagcc cgatctcgcc agcgccgccg cggtgcgcgc ctacaagcaa ctcaagctcg
     2821 ccgagcgcgc attccgcacc ctcaaggaca cgctggagat ccgccccatc taccaccacc
     2881 tcgaagcacg cgtcagagcc cacgtgttcc tgtgcatgct cgcctactac ctcagcttcg
     2941 agctgcgcac ccggctcacg ccgctgctct ttgacgacga aaccccgctc gcccccaccg
     3001 accccgtcgc ccccgccatc cgctcacccg ccgcgctcac caaggccggc agcgcccgca
     3061 cccgcgacgg ccaccccgcc cacaccctcc ccgacttact ctcagagctc ggcacgctct
     3121 gccgcaacca cctacgcatc ggcacaagcc accacacctt cacccaactc accgaaccca
     3181 caacgctcca ggcgcgcgcg ctcgaactcc tggacatcaa gcccgccaag tagacagaac
     3241 ccgccgcccg cccccccacc gaatcccgcg ccacaacacg ggattcagca tctcaacggc
     3301 gaaaacttcc gcctagacgg aagttttcgg ccctgaatgt gtgaagcccg cgctgtgcgc
     3361 gggcttgggg tcgtggctgc tgggtcggct ttggctacgt ttggagggtg atgtcgagga
     3421 gttcgagggc gcgggcttgg agtg
//
LOCUS       DASUBI010000600         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_628821, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000600 DASUBI010000000
VERSION     DASUBI010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628821"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFU72_11635"
     CDS             complement(<1..1289)
                     /locus_tag="VFU72_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424185.1"
                     /translation="MSRWKRQATKRRSIYASLVALALLLVASLPAMALSKPTATKTNM
                     PYGWGDSGNSFSWSMAWFNGKLYVGTGRYTNCVSFATYAFYDIADYSTNPAPGVTCPP
                     DWMDLNLRAEIWQYTPETNTWLRVYQSPTVPNPERPGKQIAADLAYRGMVVYHNQLYV
                     LSDTPDEIVPELQTTNPPHILRTSDGTHFSALNIAPFQILYPDSQNNGQLTKFYPMGY
                     RSYAVFNDQLFVTAPRSLTGDGPLLRVNDPEGPNPTLTQLTPGNMQIFEIEPFNNQLY
                     LGGASKANGYFVARTDAVGDNPAINMVVTNGAGRGASVNSVVSMHVYNGGLYVGASGW
                     YAGTRSSELIVVRPDDSWDLVVGAARQTDQGTKAPLSGFGDGFGNPFNVHFWRMEDSQ
                     GELYLGTNDASWMLAKYPLANRLLSWQYGFDVYYSPDG"
     gene            complement(1349..>2719)
                     /locus_tag="VFU72_11640"
     CDS             complement(1349..>2719)
                     /locus_tag="VFU72_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424186.1"
                     /translation="SEQVGGTPRRFLLACWPFPGHIRPFLSLAVALREAGHTVAVYTG
                     ASVAPLVEGEGLTCFPFRGVSEDDAYANIAAIDSVPQGGRPGSRLLLKIFRDWMIETI
                     PGQIADLQPLIDDWRPDVIVTETAMLGPTLILWERTGIPVAIQSTMLGCLIPGHDIPA
                     PGFGLRPPRNAPERLVQRALVRLGGLAGRGLRRRVDAIRAEYGLAPLDVTVNEYGARL
                     PLYLIGSLRELDYDRHDLPPSVHYVGPYPWEQARAGVEDAPAWLASLSTERPWIHVTE
                     STLRYGDPFLLRTAVAALAGGPWEVILTTGRQRDPAELGLAVADNIHLAQWIPHDALL
                     PRCAAMISSGGAGTVLAALRAGIPQVIVPTTWDKPDNARRLATAGAGLSLDPRRCTPE
                     RLRAAVEEVTGESHYRRRSQELAVRLAAAAGPARSVALLEALIPAPGVVASGSSSGSS
                     SDSGWT"
BASE COUNT          425 a          881 c          975 g          438 t
ORIGIN      
        1 ccgtcggggc tgtagtagac gtcgaagccg tattgccagg acaggagccg gttggccagc
       61 gggtacttcg ccagcatcca actggcgtcg ttggtgccca ggtagagctc gccctgggaa
      121 tcctccatgc gccagaagtg gacgttgaac gggttgccga agccgtcgcc gaagccgctg
      181 agcggcgcct tggtaccctg atccgtctgc cgggcggcgc cgacgaccag atcccagcta
      241 tcgtccggcc ggaccacgat cagctccgag gagcgggtgc cggcgtacca gccgctggcg
      301 ccgacgtaga ggccgccgtt gtagacatgc atgctgacga cggagttgac gctggcgccg
      361 cggcccgcgc cgttcgtcac gaccatgttg atggccgggt tgtcgcccac ggcgtcggtg
      421 cgcgccacga agtagccgtt cgccttgctg gcgccgccga gatagagctg attgttgaag
      481 ggctcgatct cgaagatctg catgttaccg ggggtgagct gggtcagggt cggattcggc
      541 ccctcgggat cgttgacccg cagcagcggg ccgtcgcccg tcagcgagcg gggcgcggtg
      601 acgaagagct ggtcgttgaa gaccgcgtag gagcgatagc ccatcggata gaacttggtc
      661 agttgcccgt tgttctgcga atccgggtac aggatctgga acggcgcgat gttgagcgcg
      721 ctgaaatggg tgccgtcgct ggtacgcagg atatgcggcg gattggtcgt ctgcagctcc
      781 ggcacgatct catcgggggt atcgctgagg acgtagagct ggttgtggta gaccaccata
      841 ccgcggtagg ccagatccgc ggcgatctgc ttgcccggcc gctccgggtt tggcaccgtc
      901 ggtgactggt agacgcgcag ccaggtattg gtctccgggg tgtactgcca gatctcggcg
      961 cgcagattga gatccatcca gtcgggcggg caggtgacac ccggcgccgg gttggtcgag
     1021 taatccgcga tatcgtagaa ggcgtaggtc gcgaacgaaa cgcagttcgt gtagcggccc
     1081 gtacccacgt acagcttgcc gttgaaccag gccatggacc aggagaagct attccccgag
     1141 tcgccccagc catacggcat gttggtcttg gtcgccgtcg gtttgctcaa cgccatggcg
     1201 ggcagcgacg ccaccaacag gagcgccagc gcgaccaggc tggcgtagat cgaacgccgc
     1261 ttcgttgcct gtctcttcca gcggctcatg cgcattccct caactccacc ttctcgtgtt
     1321 gccggtgctc ggccggtggc cggaccgtct acgtccaacc tgaatctgaa cttgaacctg
     1381 agcttgaccc tgaggcgact acccctgggg ctggaatcag tgcttctaag agcgcgacgg
     1441 atcgcgcggg tccggctgcc gccgccaggc ggaccgccag ctcctgcgac cggcgccggt
     1501 aatgagactc gcccgtcacc tcctctaccg ccgcgcgcaa gcgctcggga gtacagcgcc
     1561 gtgggtcgag gctgaggccc gcgcccgcgg tggccaatcg tcgtgcgttg tcgggtttgt
     1621 cccaggtggt cggcacgatc acctggggga tgccggcgcg cagcgccgcc agcacggtgc
     1681 ccgcgccgcc ggagctgatc atggccgcgc agcgcggcaa gagcgcgtcg tgcgggatcc
     1741 actgcgccag gtgaatgttg tccgccaccg ccagccccag ctctgccggg tcgcgctggc
     1801 ggccggtcgt caggatgacc tcccacggcc cgccggccag cgccgccacc gccgtgcgca
     1861 gcaagaatgg atcaccgtag cgcagggtgc tctcggtgac gtggatccag ggccgttcgg
     1921 ttgagagcga cgccagccac gccggcgcat cctccacacc ggcgcgcgcc tgctcccagg
     1981 gatacggccc gacgtagtgg acggatgggg gtaggtcgtg gcggtcgtag tccagctcac
     2041 gcaggctgcc gatcaggtag agcggcagcc gggcgccgta ttcgttgacc gtgacgtcga
     2101 gcggcgcgag gccgtactcg gcccggatgg cgtcgacgcg ccggcgcagg ccgcggcccg
     2161 ccagcccgcc gagccgcacc agcgcgcgct ggacgaggcg ctccggcgcg ttacgcggcg
     2221 gccgcagccc gaatccgggc gcggggatat cgtgccccgg gatcaggcag ccgagcatgg
     2281 tcgactggat cgcgaccggg atgccggtgc gctcccagag gatcagcgtc gggccgagca
     2341 tggccgtctc ggtcacgatg acgtcgggcc gccagtcgtc gatcaggggt tgcaggtcgg
     2401 cgatctgccc cgggatcgtc tcgatcatcc agtcgcggaa gatcttgagg agcagtcgcg
     2461 agccgggccg cccgccctgg ggcacgctgt cgatggccgc gatattggcg taggcgtcgt
     2521 cctcggagac cccgcgaaag gggaagcagg taagtccctc accctcgacc aggggcgcca
     2581 cgctggcgcc ggtgtagacg gcgacggtgt ggccggcctc gcgcagcgcg acggccaggc
     2641 tgagaaaggg gcgaatgtgc cccggaaagg gccaacaggc gagcaggaag cgccgcggcg
     2701 tgccgccgac ctgctcgct
//
LOCUS       DASUBI010000601         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1312437, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000601 DASUBI010000000
VERSION     DASUBI010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312437"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFU72_11645"
     CDS             complement(<1..311)
                     /locus_tag="VFU72_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424187.1"
                     /translation="MPETSVAALPRAILVDLDGTLALLNERGPYDYVRCHLDVVNEPV
                     KQVVLAYRACYGKVLIISGREDVARDMTAAWLERHEIPYDGLFLRRRGDRRRDTVLKR
                     E"
     gene            542..1750
                     /locus_tag="VFU72_11650"
     CDS             542..1750
                     /locus_tag="VFU72_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5424188.1"
                     /translation="MATREELRQRILDAIDAHRDEIVAVGEEIRQNPEIGYQEFKSSQ
                     LLASAFRGHGYEVEKPAGGLETAFVASKHGYKDGPHVAVLAEYDALAGIGHGCGHNLI
                     AASGLAAAIGMNAVIDELPGIFEVIGTPAEEGGGGKIRLENAGVFDDVDAALMVHHAG
                     NHGGAPLEYPTGTCLARALFTLEYFGKPAHSAGDPYNGVNALNAMIKFFTGVDALRQH
                     VRMETRLHGIITDGGEQPNVVPKYSRARFYVRCDSQGYLQEVLQKVYQIAEGAALMTG
                     CEVKIEEDNVTFDMRPSYEIGHRYQDNMREVGLDLNGREGRGMHSTDFGNISYKLPSV
                     TGSFAISHEPIPGHSQQVVDASGSEYGYDQLIKAAKAMALTAFDLLTEPELLASAKEE
                     HAHWTERYEG"
     gene            2024..>3645
                     /gene="selB"
                     /locus_tag="VFU72_11655"
     CDS             2024..>3645
                     /gene="selB"
                     /locus_tag="VFU72_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5424189.1"
                     /translation="MTGAQHQTFVIGTAGHVDHGKSTLVKALTGIDPDRLREEKEREM
                     TIDLGFAWLTLDSGRQVSVVDVPGHERFIKNMLAGVGGIDAALLIVAADEGPMPQTDE
                     HLHILDLLHVSRGLVVLTKIDLVDSEWRELVAEEVRDRLKGSALQEAPLVPVSAITGA
                     GLDELRAALDELLSTVPAHVEGGRARLPIDRVFTMAGFGTIVTGTLLDGSLALGQEVE
                     LQPSGQRARVRGLQTHRAKVERALPGSRTAVNLSGLAVEQVARGEVLTTPGWLMPTQL
                     LDARLRLVADAPAALEQNDAVDFFVGAAETLARVTLLDRERIEPGDDAWVQVRVETPI
                     AVAQGDRFIVRRPSPSLTIGGGVVVDPHPRRHRRFRPEVIAALEVRERGTPEEIVEQQ
                     LGGAPWELRALLAATALSEPDVRRAIDSLLAAGRVVALRRSESSGALPPSTYVITRGA
                     LEGTVERIRGELAAYHQRNPLRRGMSREEVRARLGLAGRPFDEIVARAAAEGSLEGDA
                     ETLRLPDHTIRFDPEQQARVDRYLAALRARPHT"
BASE COUNT          610 a         1263 c         1228 g          544 t
ORIGIN      
        1 atctcgcgtt tgagcacggt gtcgcgacga cgatcgcccc ggcgccgcag aaagagcccg
       61 tcgtacggaa tctcatgacg ctcgagccag gccgcggtca tgtcccgcgc tacgtcctcg
      121 cggccggaga tgatcaatac cttcccgtag caggcacgat aggcgaggac cacctgcttg
      181 accggctcat tgaccacgtc caggtggcag cgcacgtagt cgtaggggcc gcgctcgttg
      241 agcaaagcca acgtgccgtc gagatcgacg aggatcgccc gagggagtgc cgcgaccgaa
      301 gtctcaggca ttgatggcct gcatcagaga tacaagagtg tctggcgtcg ccagcgacag
      361 cgccgagcac ggctcgagac cggcagtcat gctcacctcc tcatctgagt ggcgcctcag
      421 cggcctcggc accgcgagtt tcgttcccaa actgtgcgct cgccttccgg ctagccgtcc
      481 cgccgggccg atggtatcgt gcccgctggg agcgaattgc cgagaactac aggaggagat
      541 catggctacg cgcgaggagc ttcgccagcg catcttggac gcgatcgacg cgcaccgcga
      601 cgagatcgtc gcggttggcg aggagatccg ccagaacccg gagatcggct accaggagtt
      661 caagtcgtcg cagctcctgg cctcggcctt ccgcgggcac ggctacgagg tcgagaagcc
      721 ggccggcggc ctcgagaccg ccttcgtcgc cagcaagcac ggctacaaag acggaccgca
      781 cgtcgcggtc ctggccgagt atgacgcgct ggcagggatc gggcacggct gcggtcacaa
      841 cctgatcgcg gcctcgggcc tagccgccgc catcggcatg aacgccgtga tcgacgagct
      901 gcccggcatc ttcgaggtga tcggcacccc ggcggaagag ggcggcggcg gcaagatccg
      961 gctggagaac gccggcgtct tcgacgacgt ggacgcggcg ctgatggtgc accacgccgg
     1021 caaccacggc ggcgcgccgt tggagtaccc caccggcacc tgcctggcgc gggcgctctt
     1081 caccctggag tacttcggca aaccggcgca ctcggccggc gatccctaca acggcgtcaa
     1141 cgcgctcaac gcgatgatca agttcttcac cggcgtcgac gcactgcgcc agcacgtgcg
     1201 catggagacg cgcctgcacg gcatcattac cgacggcggc gagcagccca acgtggtgcc
     1261 gaagtactcg cgtgcccgct tctacgtccg ctgcgactcg cagggctatc tccaggaggt
     1321 gctgcagaag gtctaccaga tcgccgaggg tgcggcgctg atgaccggct gcgaggtcaa
     1381 gattgaggaa gacaacgtga ccttcgacat gcgccccagc tacgagatcg gccaccgcta
     1441 tcaggacaac atgcgcgagg tcggcctcga tctcaacgga cgcgagggcc gcggcatgca
     1501 ctcgaccgac ttcggcaata tctcctacaa gctgccgtcg gtcaccggct cgttcgccat
     1561 cagccacgag ccgatccccg gccactcgca gcaggtggtg gacgcctcgg gttcggagta
     1621 cggctacgac caactgatca aggccgccaa ggccatggcc ctgaccgcct tcgacctgct
     1681 caccgagccg gagctgctgg cgagcgccaa ggaggagcac gcccactgga ccgagcggta
     1741 cgaagggtag acagccgcac gcgtcgtcca catttggagc cgcgtgcggc acggatcctt
     1801 cgacaaggtc aggatggcag gagaaagggg atgacctgcc ggacgaggat gcgcctcgcc
     1861 cgatgccgat cctccgcgtc agccccgtat tcatcctgtc attccgaccg aagtggaggg
     1921 atccgtgctg acgcggcacc caacactctt gattgcctcc gcgcccctcc cgcctgctag
     1981 ccccgggcta taatctggcc ggtcacagcg gggtggagga agcatgaccg gcgcgcaaca
     2041 ccagacattc gtcatcggca cggccggcca cgtcgatcac ggcaagtcga cgctggtcaa
     2101 ggcgcttacc ggcatcgacc ccgaccggct ccgggaggag aaggagcggg agatgacgat
     2161 cgacctcggc ttcgcctggc tgacgctcga cagcggtcgc caggtgagcg tggtcgatgt
     2221 ccccggccac gagcgcttca tcaagaacat gctggccggt gtcggtggga tcgacgccgc
     2281 cctgctcatc gtcgccgccg acgaggggcc gatgccgcag accgatgagc acctgcacat
     2341 cctcgatctc ctgcacgtca gtcggggtct ggtggtgctg accaagatcg acctggtcga
     2401 ctccgagtgg cgcgagctgg tggccgaaga ggtccgcgat cggctcaagg gttcagcgct
     2461 ccaggaggcg ccgctggtgc cggtctccgc cataaccggc gctgggctag atgagctgcg
     2521 cgcggcgctg gacgagctgc tgagtaccgt gccggcgcac gtcgaaggtg gccgtgcgcg
     2581 cctcccgatc gaccgcgtct tcaccatggc aggcttcggc acgatcgtca ccggcacgct
     2641 gcttgacggc tcgctggcgc tcggccagga ggtcgagttg cagccctcgg gacagcgcgc
     2701 gcgcgtgcgc ggcctgcaga cgcaccgcgc caaagtcgag cgcgctctgc caggctcgcg
     2761 caccgccgtg aacctctcgg ggctggctgt cgagcaggtc gcgcgtggcg aggtcctgac
     2821 cacgcccggc tggctgatgc cgacccagtt gctggacgcg cgcctgcggt tggtcgccga
     2881 cgcgccggcc gcgctggagc aaaacgacgc ggtcgacttc ttcgtcggcg ccgccgagac
     2941 gctcgcccgc gtaacgctgc tcgaccgcga gcgcatcgag ccgggtgacg atgcctgggt
     3001 gcaagtgcgt gtcgagaccc cgatcgcggt cgcccagggt gaccgcttca tcgtccggcg
     3061 cccctcaccc agcctcacca tcggcggcgg ggtcgtggtc gatccgcatc cccggcgaca
     3121 ccgacggttt cggcccgagg tgatcgccgc gctggaggtg cgtgagcgcg gcacgcccga
     3181 ggagatcgtc gagcaacagc tcggcggtgc gccgtgggag ctgcgcgcgc tactcgccgc
     3241 caccgctctg tccgagcctg acgtgcggag ggcaatcgac tcgctgctcg cggcgggccg
     3301 cgtcgtcgcc ctgcgccgct ccgaatcctc cggagccctc ccgcccagca cctacgtcat
     3361 cacgcgtggt gcgctcgaag gaacggtcga acgcatccgc ggcgagctag ccgcctatca
     3421 ccagcgcaat ccgctgcggc gtggcatgag ccgcgaggaa gtccgcgcgc ggctgggttt
     3481 ggccggtcga cccttcgacg agatcgtggc gcgcgccgcg gccgagggca gcctggaggg
     3541 cgatgccgag accctgcgtc tccccgacca tacgatccgc ttcgatccgg aacagcaagc
     3601 gcgggtcgac cgctacctgg cggcgctccg cgctcggccg cacac
//
LOCUS       DASUBI010000602         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_356741, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000602 DASUBI010000000
VERSION     DASUBI010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356741"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /locus_tag="VFU72_11660"
     CDS             <1..2082
                     /locus_tag="VFU72_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5424190.1"
                     /translation="GREFVAVLVGLSPARRSRWAAPGLLLIVALVLALVLSVRPANAA
                     SAPADILLGLAPGAPLDGLLDAAGALAAPRALGQPGVYQVLVADQARALASLRGNPFV
                     RYAEPDGVLALAQGVARTQDQDSNGSDNHWGLDRIGAAEARQSDTGAGVTVAVLDTGV
                     SASHPELAGRVLPGYDFVDGRPGAADDAGHGTYVAGIIGAATQSDGAGGVAPGVTILP
                     VKILNQSGVGSTANFVAGINYAVAQGARVINISAGGIDDSQALEDALRNAEAHGAVVV
                     AATGNDGVDAVSYPAAVTSVLAVSASDQSDTVPGFATYGAYVDLAAPGVDIASTWWSA
                     AGGNGRTVASGTSAAAPFVSGAAALLLGARPGLDPAGVRQLLEESAVDIEQPGIDARS
                     GFGRLDAALAMRLAAVTPLTGTVSLPLVDGTPHLTMSAVGFAPNEPVTLVLQEDLGSQ
                     SFVGYSADGTGALGVDLGPAARFPSDTLTVSAVGSNSGRLARGELQLTRTPAAPAFAP
                     VAPVPSTGDRLYFPETGHTLSGGFLAYWQAHGGLAVFGYPISEEFDEQNPDTGQFDTV
                     QYFERNRFEYHPELAGTPYAVSLGRLGSELAPQVFPAAATPLGPDPGTRYFPETGHTL
                     GGAFLGYWQARGGLAIFGYPTSEPFEQNGRVVQYFERARFELHPEPDGALLVLLGRLG
                     VDLAYQNGYLR"
BASE COUNT          305 a          866 c          878 g          383 t
ORIGIN      
        1 ggaagagagt tcgtagccgt gctggtaggt ctctcgccgg cgcgtcgctc gcgctgggcg
       61 gcgccggggc tgcttctgat cgtcgcgctg gtgctcgcgc tggtgctcag cgttcgccca
      121 gcgaacgcgg cctccgcgcc cgccgacatc ttgctcggtc tcgcccccgg tgcgccgctc
      181 gacggtctcc tcgacgcggc gggagcgctg gccgcgccgc gcgccctcgg tcagcctggc
      241 gtctatcaag tgctggtggc tgaccaggcg cgggcgctgg cctcgctgcg cggcaacccg
      301 ttcgtgcgct acgccgagcc ggacggcgtg ctggcgctgg cgcagggtgt ggcacggacg
      361 caggatcagg acagcaacgg ctcggacaac cattgggggc tcgaccgcat cggagcggcc
      421 gaggcccggc agagcgacac cggcgccggt gtcaccgtgg cggtgctcga taccggtgtc
      481 agcgccagcc acccggagct ggccgggcgc gtgctgccgg gctacgactt tgtcgacggt
      541 cgacccggcg ctgccgacga cgccggccac ggcacctacg tcgccggaat catcggcgcg
      601 gcgacgcagt ccgatggcgc gggtggcgtc gccccggggg tcacgattct gccggtcaag
      661 atcctcaacc agagcggtgt cggctcgacc gccaacttcg tggccggcat caactacgcc
      721 gtcgcgcaag gcgcgcgcgt gatcaacatc agcgccggcg ggattgacga ctcgcaggcg
      781 ttggaggacg cgctgcgcaa cgccgaggcg cacggcgccg tggtggtggc ggccactggg
      841 aatgacggcg tcgacgccgt gagctacccc gcggcagtga cgagcgtgct ggccgtcagc
      901 gccagtgacc agagcgacac ggtccccggc ttcgccacct acggcgccta cgtcgacctg
      961 gccgcgccgg gcgtcgatat cgccagcacc tggtggagcg ccgcgggcgg gaatggacgc
     1021 acggtcgcca gcggcacctc tgcggccgcc cccttcgtct cgggagccgc ggcgctgctc
     1081 ctgggcgcgc ggccggggct ggatccggcc ggcgtgcgcc agttattgga ggagagcgcg
     1141 gtcgatatcg agcagccggg catcgacgct cgcagcggct tcgggcggct cgacgcggcc
     1201 ctggctatgc gcctggccgc cgtgactccg ctgaccggca cggtcagctt gccgctcgtt
     1261 gacggcacgc cgcacctgac catgagcgct gttggcttcg cgccgaatga gccggtgacg
     1321 ctggtgctgc aggaagatct cggcagccag tcgttcgtcg gctacagcgc cgacggtacg
     1381 ggcgcgttgg gcgtcgacct cggcccggcg gcgcgcttcc cgagcgacac gctgaccgtg
     1441 tcggcggtcg gttcgaactc cgggcggctg gcgcgcggcg agctgcaact gacgcgcacg
     1501 cccgcggcgc cggccttcgc gccggtggcg ccggttcctt cgaccggcga ccggctctac
     1561 ttccccgaga cggggcacac cctctccggc ggctttctcg cctactggca ggcgcatggt
     1621 gggttggccg tcttcggcta cccgatctcc gaggagttcg acgagcagaa cccggacacc
     1681 ggtcagttcg acacggtgca gtacttcgag cgcaatcgct tcgaatacca tccggagctg
     1741 gccggcacgc cctacgcggt ctcgctgggc cggctcggca gcgaactggc gccccaggtc
     1801 ttccccgccg cggccacgcc cttggggcca gaccccggca cgcgctactt ccccgagacc
     1861 gggcacacgc tcggcggcgc gttcctgggc tactggcagg cacggggcgg gctggcgatc
     1921 ttcggctacc ccaccagcga gccgttcgag cagaacggcc gggtggtgca gtacttcgag
     1981 cgcgctcgct tcgagctgca ccccgaaccc gatggcgctt tgctggtcct cctgggccgc
     2041 ttgggcgtcg atctggccta ccaaaacggg tacctgcgtt agctcgcggc gccccgttcg
     2101 taccgctgga aggggcgctc acgcgggtac cttggtgtga tcctttaccg ggtatctcag
     2161 tacaagggga ccatcgcctc ggccggcgcc ggacggtagc gtagaggtat ggtgattacg
     2221 cagcagttcc ggaccgtccc ggcaagacat ggcgttctcc aacccctggt ttgccactac
     2281 gcgggcccct caagcgcggc acagcgccga gcggcgcccg gcgtcgccct cctcccccta
     2341 cggccggcgt ggctgcggcg tggtgtgcgg ttgccggcct ggctgctgtc gctgacggtc
     2401 ggtgtctctc tggtcggcgc gatcgcgttg cc
//
LOCUS       DASUBI010000603         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_917236, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000603 DASUBI010000000
VERSION     DASUBI010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917236"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2140
                     /locus_tag="VFU72_11665"
     CDS             <1..>2140
                     /locus_tag="VFU72_11665"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEU5424191.1"
                     /translation="GTTATIDGSSIVSNTAGPSAASGNENGGGLYAVRTSIATITNST
                     ISDNTAATDGGGIFNASAMAAIQNTITLVNVTITANSAGRNSGGLDNFAVASIFNTVV
                     AGNTAAASSPDCLNASGHEITSQGYNLIGIIDGCVITGTTTGNQTGTLAAPLDPKLSP
                     LGFYGGPTPTHRQLLHSPLIDRGGNTGCPATDQRGVKRPGQGTGQAICDIGAVELSLG
                     VSQTPDNTSWPHARDVALSGGNGNSGDDLTAPDQSRWYKFLVKPNSHVTVTLTNLPAN
                     YDLTLYKDIGAAFRAFQTLSSSQDLAQLSAEFAPDAFSPDAYSPDAFSPDAFSPDAYS
                     PDAFSPDAFSSDVFAPDAFSPDAYSPDAYSPDAFSPDAFSPDAYSPDAFSPDAYSPDA
                     FSPDAYSPDAYSSAQTRSLIGVSAFNGDASEGISLNTWDNTGYFYVRVRGRNGADVPS
                     APPFTVTINQTYTACSAFDGSSLPATSTAAVAGNYQTIILTDMARMSAQPGNSSADLS
                     ALTSKLNSFAASVGGVVVDVHNDARVSAANTQADANPGCPNIKNQLADSIKAIVDGYR
                     QKNAGLKYIVVVGDDDVIPFYRYPDEALLASEQDFVPPVKDSSASQASLRLGYVLSQD
                     RYGSAINLSYNITTLPIPQLAVGRLVETMHDASTMLDAYAKTASGVITPSSALVTGYD
                     FLADDAQAVQTQLSQGLGAGGKIDSLITPED"
BASE COUNT          386 a          832 c          618 g          304 t
ORIGIN      
        1 cggcaccacc gccaccatcg acggcagctc catcgtcagc aacactgccg ggccgtccgc
       61 cgccagcggc aatgagaacg gcggcgggct ctacgccgtc cgcaccagca tcgccaccat
      121 caccaacagc accatcagcg acaacaccgc cgccaccgac ggcggcggca tcttcaacgc
      181 cagcgcgatg gcggccatcc agaacacgat caccctggtc aacgtcacca tcaccgccaa
      241 tagcgctggc cgcaatagcg gcgggttgga taacttcgcc gtggcctcga tcttcaacac
      301 ggtcgtggcc ggcaacactg ccgccgccag ttcgccggac tgcctcaacg ccagcggcca
      361 cgagatcacg tcgcagggct acaacctgat cggcatcatc gacggctgcg ttatcaccgg
      421 cacgaccacc ggcaaccaga ccggcacgct cgcggcgccg ctcgatccga agctgagccc
      481 gctcggcttc tacggtgggc cgacgcccac ccaccgtcag ttgctgcata gcccgctcat
      541 cgacaggggc ggcaacaccg gttgcccggc aaccgaccag cgcggcgtca agcggcccgg
      601 ccaggggacc ggccaggcga tctgcgacat cggcgcggtc gagctgagcc tcggcgtcag
      661 ccagaccccc gacaacacgt cctggccgca cgcccgtgat gtcgcgctct ccggcggcaa
      721 cggcaattca ggcgacgacc tgaccgctcc ggatcagtcg cgctggtaca agttcctggt
      781 caagcccaac agccacgtca ccgtcaccct gaccaacctg ccggccaact acgatctgac
      841 gctctacaag gacatcggcg ccgccttccg ggccttccag acgctctcct cctcgcagga
      901 tctggcgcag ctcagcgccg agttcgcgcc cgacgccttc tcgcccgacg cctattcgcc
      961 ggacgccttt agcccggacg ccttctcgcc cgatgcctat tcgccggacg ccttcagccc
     1021 cgacgcgttc agctcggatg tcttcgcgcc cgatgccttt agcccggacg cctactcgcc
     1081 ggacgcgtac tcgccggacg ccttcagccc cgacgccttc tcgccggacg cctactcgcc
     1141 ggacgcgttc agccccgatg cctacagccc cgacgccttt agcccggacg cctacagccc
     1201 cgacgcctac tctagcgcgc agacgcgcag tctgatcggc gtctcggcct tcaacggcga
     1261 tgccagcgag gggatcagcc tcaacacctg ggataacacc ggctacttct acgtgcgcgt
     1321 gcgcggccgc aacggcgctg atgtgcccag cgcgccgccg ttcacggtga cgatcaacca
     1381 gacctacacc gcctgcagcg cgttcgacgg cagttcgctg ccggcgacca gcacggccgc
     1441 cgtggcgggc aactaccaga ccatcatcct gaccgacatg gcgcgcatga gcgcgcagcc
     1501 cggcaactcg tccgccgacc tgagcgcgct gacgagcaag ctgaacagct tcgcggcttc
     1561 ggtcggcggc gtggtcgtcg atgtccacaa tgacgcgcgg gttagcgcgg ccaacacgca
     1621 ggccgacgcc aaccccggct gccccaacat caagaaccag ctggccgatt cgatcaaggc
     1681 gatcgtcgac ggctatcggc agaagaacgc gggtctcaaa tacatcgtcg tcgtcggcga
     1741 cgacgacgtg atccccttct accgctatcc ggacgaggcc ctgctggcca gcgagcagga
     1801 cttcgtgccg ccggtcaagg actccagcgc ttcgcaggcc agcctgcggc tcggctacgt
     1861 gctgagccag gaccgctacg gctccgcgat caacctctcc tacaacatca ccaccctacc
     1921 gatcccgcag ctggcggtcg gtcggctggt cgagacgatg cacgacgcca gcaccatgct
     1981 cgacgcctac gccaagaccg ccagcggcgt gatcacgccg tcgagcgccc tggtcaccgg
     2041 ctacgacttc ctggccgacg acgcgcaggc ggtgcagacg caactgtccc agggtctcgg
     2101 cgccgggggc aagatcgact ccctgatcac gccggaggac
//
LOCUS       DASUBI010000604         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_70999, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000604 DASUBI010000000
VERSION     DASUBI010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70999"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFU72_11670"
     CDS             complement(<1..899)
                     /locus_tag="VFU72_11670"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5424192.1"
                     /translation="MSGPSVGLRWLSLGTLAGGAPLRLPLHTLRGTAPGPTVGICAGI
                     HGDEIQPIEVVRRVAGVLGQSELSGTVRLLPVANPLAFAGLSRHTPQDRQNLNRIFPG
                     DPHGWLTEQLAHAIAGAFLPGLDALIDLHAGGLLPTVDYVYILNDEALSRAFGVRVMF
                     RTPRSFAGTLGSLAVARSVRTVVSELGGGQLADEEYLARGVAGVLSALRQLGTLPGEP
                     APHPEPLITERLETLRPHQGGLLVPEVRVPDLGKVVPRGTLLGRVYDPYTFAGLERFE
                     APFARTILILLRGAVSGVQIGDYA"
     gene            complement(909..2624)
                     /locus_tag="VFU72_11675"
     CDS             complement(909..2624)
                     /locus_tag="VFU72_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5424193.1"
                     /translation="MPKRECESYRTLISAYLDGDLDSAERDRLFAHLETCGECAETLE
                     TYQRIRAGIRDLPRFAPPPNLGHSIRRSTVERPPASRWSRFFGFGGARLGLSTAGVLA
                     LLIVACSFLLVRGYDQTVSPAVAASAPGARQEWPTTQPIRVQFSKPMDHTSVEGSLRI
                     SPPGERDRLPLSWDGNTLILGASRTASVPLLPNTDYTIMVLADARDQYGNTLGQVWTL
                     SFHTGSATALTQPTVTHTPTPSPTPRGEQSAIQVPPTATATALARPGVPATSPPAATN
                     PPLSVNPTTPDSGPVTGSGQPTAAPAPPAPTPKPASTATPTPAPTPAATETPKAQPTV
                     QPTVAPTAAATTAPTPAPTATAEKPTQVTTAAPTGSPATATATSAAETPTPSGIPVVG
                     SLGSVYWSNADVQTKLGLPSASEVKVNTAELSFQHGVMYERYDTGRIYVLLTDGQWSS
                     AEDTWTSNDSPGGQTCGSNADNLFQPTKGFYKVWSADPKLQQAIGCAVQPTTTLAANN
                     VQEFANGEMLYGDQGFVYVVYSDNSWKLYPDTSGHSSLLTPTPEPAATTPTPTPATPS
                     TAAAP"
     gene            complement(2588..3208)
                     /locus_tag="VFU72_11680"
     CDS             complement(2588..3208)
                     /locus_tag="VFU72_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5424194.1"
                     /translation="MDGYAVLPVDEATWVDRAKEGDQAAFEAIFQRYERQIYGFIYRM
                     MGNPEDASDLTQECFIRAYKALPQTSVDLNVSAWLHRIASNACLDVLRRRQRIRWLPW
                     ETHKHEHLLHGKPIDDPERTALSSETQVLVQRILNQMSPRNRMALVLREYEGMSCEDI
                     GEIMGLSRSAVKSVLFRGREEFRKLYRAVEDEEPVCRSGSVSPTGR"
BASE COUNT          543 a         1096 c         1243 g          556 t
ORIGIN      
        1 gcgtagtcac cgatctggac gccggaaacc gccccgcgca gcaggatcag aatcgtgcgc
       61 gcgaagggcg cttcgaagcg ctccagcccc gcgaacgtgt agggatcgta aacccgcccc
      121 agcagcgttc cccgcggtac caccttgccc aggtcgggca cgcgcacctc gggcaccagc
      181 agcccgccct ggtgcgggcg cagcgtctcc aggcgctcgg tgatgagtgg ctcgggatgc
      241 ggcgccggct cgccgggcag cgtgccaagc tggcgcagcg cggagagcac gccggcgacg
      301 ccgcgcgcca gatactcctc gtccgcgagc tggccgccgc caagctcgga taccacggtc
      361 cgcacgctcc gcgcgaccgc cagcgacccg agcgtgcccg cgaagctgcg cggcgtgcgg
      421 aacatcacac gcaccccaaa ggcccgcgag agcgcctcgt cattcaaaat atagacataa
      481 tcgacagtgg gcaagagccc gcccgcgtgc aggtcgatga gcgcgtcgag cccgggcagg
      541 aacgcgccgg cgatggcatg ggccaattgc tcggtaagcc agccgtgggg gtcgcccggg
      601 aagatgcggt tgagattctg ccggtcctgc ggcgtatgcc ggctgagccc ggcgaaggcc
      661 aggggattgg cgaccggcaa gagacgcacg gtgccggaaa gctcggattg acccaggacg
      721 ccggccactc ggcgcacgac ctcgatcggc tgtatctcgt cgccgtgaat gccggcgcag
      781 atgccgaccg tcggtcccgg cgccgtaccg cgcaaggtgt gcagcggcag gcgcagcggc
      841 gcgccgccgg cgagagtacc cagcgacagc cagcggagac ccaccgacgg cccgctcact
      901 ggttcaccct atggtgcggc cgcggtcgag ggcgttgccg gcgttggggt aggtgttgtc
      961 gcggccggct ccggcgtcgg cgtcagcagc gaactgtgcc ccgacgtatc ggggtagagc
     1021 ttccaggagt tgtcgctgta gacgacgtag acgaagccct gatcgccgta gagcatctcg
     1081 ccgttggcga actcttgcac gttgttggcg gccagcgtgg tggtcggctg tacggcgcag
     1141 ccgatcgcct gttgcagctt ggggtccgcg ctccagacct tgtagaagcc cttcgtcggc
     1201 tggaagaggt tatcagcgtt gctgccgcag gtctgcccgc ccgggctgtc attcgatgtc
     1261 cacgtatcct ctgccgagct ccattgaccg tcggtgagga gcacgtagat gcggccggtg
     1321 tcgtagcgct cgtacatcac accgtgctgg aagctgagct cggcggtatt gaccttgacc
     1381 tcggacgcag acggcaaacc cagcttggtc tgcacgtcgg cgttcgacca gtagacgctg
     1441 ccgagtgagc cgacgaccgg gatgccggag ggcgtgggag tctcagcggc gctcgtggcg
     1501 gtggcggttg ccgggctgcc ggtcggcgcc gcggttgtca cctgcgtggg cttctcggcc
     1561 gtggcagtcg gggccggtgt tggagcggtg gtggcggccg cggtcggggc gacggtgggc
     1621 tggacggtcg gctgcgcctt cggcgtctcc gttgctgccg gagtgggcgc tggggtaggc
     1681 gtggccgtgc tggccggttt cggtgtcggc gcgggcggcg cgggagcagc cgttggctgg
     1741 cccgacccgg tcaccggacc ggagtcaggg gtggtcggat tgacgctcag cggcgggttg
     1801 gtggcggctg gagggctggt ggcgggcacg ccaggccgcg cgagcgcggt cgcggtggcc
     1861 gtcggcggca cctggatcgc cgactgctcg ccgcgtgggg tcggcgacgg tgtcggcgtg
     1921 tgcgtaacgg tgggctgggt gagcgccgtg gcggaaccgg tgtggaagct cagcgtccag
     1981 acctggccga gcgtgttgcc gtactggtcg cgggcgtcgg ccaggaccat gatggtgtag
     2041 tccgtattgg ggaggagcgg tacgctggcc gtgcggctgg cgcccagaat cagggtattg
     2101 ccgtcccaac tgagcgggag tcggtcgcgc tcgcccggcg gggagatacg cagactcccc
     2161 tcgaccgagg tgtgatccat cggcttgctg aactggacgc gaatcggctg ggtggtgggc
     2221 cattcctgcc gcgcgccggg ggccgaggcc gcgaccgcgg gggagaccgt ctggtcgtag
     2281 ccgcgcacca gcaggaagga gcaggcgacg atcaaaagcg ccagcacgcc ggcggtcgag
     2341 agccccagac gtgcgccgcc gaagccgaaa aaccggctcc agcgtgatgc cggcgggcgc
     2401 tccactgtcg agcggcggat cgagtggccc agatttggcg ggggagcgaa gcggggaaga
     2461 tcgcgaatgc cagcgcggat gcgctggtag gtctcaaggg tctcggcgca ctccccgcag
     2521 gtttcgagat gcgcaaacaa ccggtcacgc tcggcggagt cgagatctcc gtcgagatag
     2581 gcggagatta gcgtccggta ggactcacac tcccgcttcg gcataccggc tcttcgtcct
     2641 ccaccgcccg atagagcttg cggaactctt cccggcctcg gaacaggacc gacttcacgg
     2701 ccgagcgcga caagcccatg atctcgccga tgtcctcgca ggacatcccc tcgtactcgc
     2761 ggaggaccag cgccatacgg ttgcgcgggc tcatctgatt caggatccgc tgcaccagta
     2821 cctgggtctc gctgctgagg gcagtacgct cgggatcgtc gatcggcttg ccgtgcagca
     2881 gatgctcgtg cttgtgcgtc tcccagggca gccagcggat gcgctgacgc cggcgcaaga
     2941 catcgagaca ggcgttggaa gcgattcgat gtagccaggc tgagacattc agatccacgg
     3001 acgtttgcgg gagcgccttg tacgcacgga tgaagcattc ctgagtaagg tcgctcgcgt
     3061 cctcggggtt gcccatcatg cgatagatga aaccgtagat ctgccgctcg tagcgctgaa
     3121 aaatcgcctc aaaggcggcc tggtccccct ccttcgcccg gtcaacccac gtggcctcgt
     3181 ctaccggtag aacggcgtag ccatccatac gaggtcctcc gcccctacta acgcacaaac
     3241 ggcatgccgg ttgtgaccaa tcccctggac ccggtctgac gctgacatcg atgtaagcgc
     3301 gcttgtccac gcgtgccggc cgcggcgcat caccgccttg cgtctggata ccgggctgct
     3361 gcttgaggac cgagacggta tccggacaac gggatctcaa ccgcgagccg ttagaccgat
     3421 ggtaccatta tctccgaa
//
LOCUS       DASUBI010000605         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1332783, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000605 DASUBI010000000
VERSION     DASUBI010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332783"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFU72_11685"
     CDS             <1..952
                     /locus_tag="VFU72_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5424195.1"
                     /translation="AVIDASGTYQTPNPLGGDGLPALGERELAGRIHYGIPDVLGRER
                     ARYAGRRVLVVGSGHSAFNTLLDLVELAREAPGTTVLWTMRRQVTSQLYGGGENDALP
                     ARGSLGRRLRRAVASDEITLFAGFKTTTLTQTDQGIVVSSATRDLPPVDEIVAVTGFR
                     PDLAPLAELRLELDPAVESPRALAPLIDPNIHSCGTVRPHGAEELKHPEADFYIAGMK
                     SYGRAPTFLMLTGYEQVRSVVAAIEGDWERARAVELVLPETGVCTLAVPTAPAPAACC
                     GTPEEAASGCCTPLPAGAGACCVESRERQLAGAGAGSCCS"
     gene            1095..2381
                     /locus_tag="VFU72_11690"
     CDS             1095..2381
                     /locus_tag="VFU72_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424196.1"
                     /translation="MKRTRLGDLYFGWPMLLGLSAAEVTSWGILYYSFSVFLDPMRDA
                     LGWSTTELTGAYSLALLLSGLTAIPVGRWLDRHGPRLLMSLGSIAAALLVVAWSSVHN
                     LVAFYAIWVGIGVVMATVFYEPAFYVVAQWFDRLRSRALTLLTFVGGFASVIYLPLAG
                     WLLRSYGWREALLILAAILLVGTLPVHALLLRRHPRDLGLEPDGKVLPAGGQPHALAP
                     SASVPQALHDPGFWWLTGSFCLVMFTAVAVTVHLIPYLIGQGYSSGFAATAAGLVGLF
                     ALPGRLIFTPLGGRIPRRYVTALIFLLQAFSIAVLLAWHSTLGVFAFVALFGSGFGAI
                     TPARAALVVELYGPGNYGSIAGMLALWVTVARAAGPFVAGVIFGLAGSYQPVFWTLLA
                     ASALAALAALQIRSNVLPDSEARAHAPASNTQATAE"
BASE COUNT          290 a          893 c          856 g          410 t
ORIGIN      
        1 cgcggtgatc gacgcctccg gcacctacca gaccccgaac ccgctcggcg gcgacggcct
       61 gccggcactc ggcgagcggg agttggccgg gcggatccat tacggcatcc ccgacgtgct
      121 ggggcgcgag cgcgcgcgct acgccgggcg gcgcgtgctg gtggtcggca gcgggcactc
      181 tgccttcaac acgctgctcg acctggtcga gctggcccgt gaggcgccgg gaacgacggt
      241 gctctggacg atgcgccgcc aagtcacctc gcagctctac ggcggcggcg agaacgatgc
      301 cctgccggcg cgcggcagcc tcggccggcg cctgcgccgg gccgttgcat cggacgagat
      361 cacgctcttc gccgggttca agacgacgac gctcactcag actgaccagg gtatcgtcgt
      421 ctccagcgcg acccgagacc tgccccccgt cgacgagatc gtggccgtga ccggctttcg
      481 cccagacctg gcgccgctcg ccgagctgcg gctggagctg gacccggcgg tcgagagccc
      541 gcgcgcgctg gcgcccttga tcgaccccaa catccacagc tgcggcacgg tgcgtcccca
      601 cggagccgaa gagctcaagc atcccgaagc ggacttctac atcgccggca tgaagagcta
      661 cgggcgcgcg cccaccttcc tgatgctgac cggctacgag caggtgcgct cggtcgtggc
      721 ggcgatcgaa ggggattggg agcgcgcgcg ggcggtggag ctggtgctac ccgagaccgg
      781 cgtctgcacg ctggctgtgc ctaccgcgcc cgcgccggct gcctgctgcg gcactcccga
      841 ggaagcggcc tccggctgct gcaccccgtt gcccgccggc gcgggcgcct gctgcgtcga
      901 gtcgcgcgag cggcagttgg cgggggccgg cgccggctcc tgctgcagct aggcgctcga
      961 tactcgcgtt cagcccctcg ccgatacgcg gggccgggta ggctcgccgg gccggcgagg
     1021 ggttgacagc ggcgacacgt gatcgtaaat taccgattgc gatggtgaag ctggggggtt
     1081 tcgtgaagcc gctggtgaaa cggacacgcc tgggcgacct ctactttggc tggccgatgc
     1141 tgctggggct ctcggcggcc gaggtgacgt cctggggcat cctctactac tcgttctcag
     1201 tcttcctgga tccgatgcgc gacgcgctcg gctggtcgac gacggagctg accggtgcct
     1261 actcgctggc cctgctcctc tcgggcctga ccgccatccc ggtcgggcgc tggctcgatc
     1321 gccacggccc gcggctgctg atgagcctgg ggtcgatcgc ggccgcgctg ctggtcgtcg
     1381 cctggtcctc ggtccacaac ctcgtcgcgt tctacgccat ctgggtcggc atcggcgtgg
     1441 tcatggcgac ggtgttctac gagccggcct tctacgtggt cgcacagtgg ttcgaccggc
     1501 tgcgcagccg cgccctgacc ctgctgacct ttgtcggcgg cttcgccagc gtgatctacc
     1561 tgccgctggc cggctggctg ctacgcagct acggctggcg cgaggcgttg ctgatcctgg
     1621 cggcgatcct actggtcggc acgctgccgg tccacgcgct gctgctgcgc cggcacccgc
     1681 gcgacctcgg gctggagccg gacggcaaag ttctgccggc gggcggccag ccgcacgcgc
     1741 tggcccccag cgcgagcgtg ccgcaggcgc tgcacgaccc cggcttctgg tggctaaccg
     1801 gctccttctg cctggtgatg ttcaccgccg tggcggtgac ggtgcacctg atcccctatt
     1861 tgatcggcca gggctacagc tccggcttcg ccgccaccgc cgccgggctg gtcggcctct
     1921 tcgccctgcc ggggcggctg atcttcaccc cgctgggcgg gcgcatcccg cgccgctacg
     1981 tcacggcgct gatcttcctc ctgcaggcgt tctcgatcgc ggtgctgctc gcctggcaca
     2041 gcacgttggg cgtcttcgcc ttcgtggcgc tcttcggcag cggcttcggc gcaatcaccc
     2101 cggcccgggc ggcgctggtg gtcgagctct acgggcccgg caactacggc agcatcgccg
     2161 ggatgctggc gctctgggtc acggtggcgc gggcggccgg tcccttcgtc gccggcgtga
     2221 tcttcggcct cgccggcagc taccagcccg tcttctggac cctactggcc gcctcagccc
     2281 tggccgctct cgccgcgctg cagatccgct cgaacgtgct gcccgacagc gaggcccgcg
     2341 ctcacgcgcc cgcgtcaaac acgcaggcga cggcggagtg acgtcggcgc gacgatcact
     2401 gtcgtcgtcc gccccgatcc cccaatcatg tcgttctgag cgcagtgga
//
LOCUS       DASUBI010000606         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1204344, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000606 DASUBI010000000
VERSION     DASUBI010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204344"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFU72_11695"
     CDS             complement(<1..182)
                     /locus_tag="VFU72_11695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424197.1"
                     /translation="MATVQRDNGGEFLAPAQNRPLKVGIILPSAGDILSGEPCRVADL
                     VAMARRAEELGFDSVWL"
     gene            443..1396
                     /locus_tag="VFU72_11700"
     CDS             443..1396
                     /locus_tag="VFU72_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEU5424198.1"
                     /translation="MSVRLGSGPFTYEALEEWESLPSGARLIECPGVAVDRDDQVYVL
                     TRNVENPVLVFNREGDHLRTFGQGVFSARTHGISVSPDGHIFCVDDGTHTVTKWTPEG
                     ELVMTIGTPNQPAPRWSGKPFNRPTHAAVSAKTGNIYITDGYGNARVHKYSPEGEWIT
                     SWGEPGIDAGQFLLPHNIAVDSQDRVIVAEREAHRVQVFDADGTVVTMWNNIFRPDGM
                     TIGPDGNIYIDELNGGGGMDDAPGLGHRVSILSPEGELLARFGHPEEGEEPGKFIAPH
                     SIAVDSHGDIYVGEVSYTIRGRRMEPPRELKSLRKLRKVNA"
     gene            complement(1839..2006)
                     /locus_tag="VFU72_11705"
     CDS             complement(1839..2006)
                     /locus_tag="VFU72_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424199.1"
                     /translation="MFLWLYAIGVWLAFALLVALARVIGNDRSGRKQPPKSMGQKLNE
                     TEFLERSMHHW"
     gene            complement(2070..2198)
                     /locus_tag="VFU72_11710"
     CDS             complement(2070..2198)
                     /locus_tag="VFU72_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424200.1"
                     /translation="MDISGRYGLGTLIFFALGMLFIALNFYAMALGIGLTHVHPWP"
     gene            2382..>3233
                     /locus_tag="VFU72_11715"
     CDS             2382..>3233
                     /locus_tag="VFU72_11715"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU5424201.1"
                     /translation="MPAEVTSAELTEPPAGTARARARLAAALTREYSISEGAVILMAS
                     FLFSAMLGAVRQVLFNAQFGAGNEASAYYAAFRLPDGLFALIAGGALSGAMIPVLVST
                     QREDGAQEWQRLINVTLTTLLAIISLVVFVAVLATPAFVSHVLAPGFDQPTTDLTVKL
                     TRIMLLQPVILAVGSVAVAILNSRAQFLLPALSIASHNLALIAGILATWIFPGLGILG
                     PTLGVVAGALIQVLILLPGLRGDRVGYRPRWDPHDARLREVVALTIPNGLAVTVEYAG
                     GIVDTAFA"
BASE COUNT          544 a         1110 c         1072 g          507 t
ORIGIN      
        1 agccagaccg agtcgaagcc gagctcctcg gcgcgccgcg ccatggctac cagatccgcc
       61 acacgacacg gctcgccgga caggatatcc cccgcgctcg ggaggatgat gccgactttc
      121 aacggacgat tctgcgcggg cgccaagaat tcccccccat tgtcacgctg cacggtcgcc
      181 atccccatgc gtccgtgcga tcgaaccgcg ggtagagcgt ctcgcgcgtt tgaaccgggc
      241 gcacccagcc agaggacagg cttgaccgaa atgcccgccg agtgtaccaa acgcgctccg
      301 cccccagcca cgttgacagc ctccgggggc gatgcgacac tgccgcgcac gacgacagcg
      361 agcgcggccg cgctggagtg cggccttgaa ccgtcggtcc gggcgcagcc aaccggatac
      421 cggcccggcg aaaggagcca tcatgagcgt gaggctgggg agcggaccct tcacctacga
      481 ggcgctcgag gagtgggaga gcctaccgtc gggggcgcgg ctgatcgaat gccccggcgt
      541 ggccgtcgac cgcgacgatc aggtctacgt actgacgcgc aacgtcgaga atccggtgct
      601 ggtcttcaac cgtgagggcg accacctgcg cacctttggc cagggggtct tcagcgcccg
      661 cacccacggt atcagcgtca gtcccgacgg tcacatcttc tgcgtcgacg acggcacgca
      721 taccgtgacc aagtggaccc ctgagggtga gctggtcatg acgatcggca cgcccaacca
      781 accggcgccg cgctggagcg gcaagccctt caatcgcccg acccacgccg ccgtctcggc
      841 caagaccggt aatatctaca tcaccgatgg ctacggcaat gcccgcgtgc acaagtacag
      901 ccccgagggg gagtggatca cctcctgggg cgagccgggg atcgacgccg gacagtttct
      961 gctgccgcac aacatcgcgg tcgatagcca ggaccgggtg atcgtggccg agcgcgaggc
     1021 gcaccgggtg caggtcttcg acgccgacgg caccgtcgtg acgatgtgga acaacatctt
     1081 ccggcccgac ggcatgacca tcggcccgga cggcaacatc tacatcgacg agctgaacgg
     1141 cggcggcggc atggacgacg cgcctggcct ggggcaccgc gtcagcatcc tcagccccga
     1201 gggcgagctg ctggcgcgct tcgggcaccc tgaggagggc gaggagccgg gcaagttcat
     1261 cgcgccgcac agcatcgcgg tcgactcgca cggcgatatc tacgtcggcg aggtctccta
     1321 caccatccga ggccggcgca tggagccgcc gcgcgagctg aagagcctgc gcaagctgcg
     1381 caaggtgaat gcgtagcggg gggtggacgg tttggcgtct acggcggagc aattgagcca
     1441 gcggcgcgcg gggttaaaac caccgcgcta accaaacgaa gcccactgaa ggggctggga
     1501 gttcacgctc tacccattgt ggcgcatggg gacgttggcg ctcgacatac gttggagaga
     1561 tccttcggct gtgacctcag catgacagga caaaggctgc tgctctccct gtcatcctga
     1621 accgcagcga cggatctctc cgcggctcca cgcggcaaca tgcctcatgc gataggtcgc
     1681 cgttgcggtc gccccgaatt tagccccttc agtgggcttc gcggactcag cgcggcgact
     1741 ttagtcccgc gcggtcgtgg actgcaccga tgtcacggag aaggccactc ggcgcccacc
     1801 aaaacgctct gcgacaccga ggccacgcgg ccggtgactc accagtgatg catgctgcgt
     1861 tcgagaaact cggtctcgtt cagcttttgc cccattgatt ttggcggctg cttgcgtccg
     1921 ctccggtcgt tgccgatgac acgggcgagc gctacgagga gcgcgaaggc gagccagacg
     1981 ccgatcgcgt agagccagag gaacatgtca cggcctcctt tggatggccg tggcccgggc
     2041 atcgctcgcg cccgggtcgc gtcgagaggc tacggccagg ggtgaacgtg ggtcaggccg
     2101 atgccgagcg ccatcgcata gaaattgagc gcgatgaaca acatgccgag agcgaagaag
     2161 atcaacgttc ccaatccata acggccgctg atatccaacg cgcatcactc ccttcatcgg
     2221 tcgatcctgc cccagcatag cacgctcttc gcgacagggc cagcgccgca tccgcaaagg
     2281 cggtaccagc ccggctggtc cggagccggc cggctgagta tcctctggcg tatcgatcgc
     2341 ggcgcgacgt cgagcgaagc ttgccggggg taggggtgga gatgcctgcg gaggtcacca
     2401 gcgcggagct gacggagccg ccggccggca cggcgcgcgc gcgtgcccgc ctggccgccg
     2461 cgctgacgcg cgagtacagc atctccgagg gcgccgtcat cctgatggcc tctttcctct
     2521 tctcggccat gctcggcgcg gtgcgccagg tgctcttcaa cgcgcagttc ggcgccggca
     2581 acgaggccag cgcctactac gccgcctttc gcctccccga cggcctcttc gccctgatcg
     2641 ccggtggcgc gctctccggc gcgatgatcc cggtgctggt cagcactcag cgcgaggacg
     2701 gggcgcagga gtggcagcgg ctgatcaacg tcacgctgac gaccctgctg gcgatcatct
     2761 ccctggtcgt gttcgtggcc gtgctggcca cgcccgcctt cgtcagccac gtcctggcgc
     2821 ccggcttcga ccagcccacg accgacctga cggtgaagct gacgcggatc atgctgctgc
     2881 agccggtgat cctggcggtg gggagcgtgg cggtcgccat cctcaacagc cgggcccagt
     2941 tcctgctgcc ggcgctctcg atcgcctcgc acaacctggc gctgatcgct ggcatcctgg
     3001 ccacctggat cttccccggt ctgggtattc tcggcccgac gctgggcgtg gtggccggag
     3061 ccctgatcca ggtgctgatc ctcttgccgg ggctgcgtgg cgaccgcgtc ggctaccgcc
     3121 cgcgctggga cccgcacgat gcccggctgc gcgaggtggt cgcgctaacg attcccaatg
     3181 gcctggcggt cacggtcgag tacgcgggcg gcatcgtcga caccgccttc gcc
//
LOCUS       DASUBI010000607         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_739987, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000607 DASUBI010000000
VERSION     DASUBI010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739987"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFU72_11720"
     CDS             <1..387
                     /locus_tag="VFU72_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424202.1"
                     /translation="AHFLKPGGTFCLVEGHPFAHVFHGEPGVLEVRYPYFEQPEPLRF
                     ETKGSYGDPESDYESVEYYWSHSLSEIVNALLGAGLALVHLREFDFLSWQMGTNMEQG
                     ADGYWRLPAGYPPLPFMFALKAVKPG"
     gene            complement(399..1397)
                     /locus_tag="VFU72_11725"
     CDS             complement(399..1397)
                     /locus_tag="VFU72_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEU5424203.1"
                     /translation="MSGHDIITIGASAGGVEALIRLVEGLPFDLPAALFVVLHFPPTS
                     TSYLPEILQRAGTLPAAQARDLEPIVPGRIYVAPPDRHLIVERERMRLTWGPKENRHR
                     PAVDPLFRSAAIAYGPRVVGVVLSGMDGDGTAGLRAIAQRGGVTVVQDPADAIFPSMP
                     ESALAYVEVDYRLPARALGGLLAELAHSPVPREDAYPVPEMLRLEHRQMMGELETFDM
                     NQFGRLTSLTCPECAGPIWELHEGELVRYRCRVGHTFLTDDMIEARSEQIEQQLWAAI
                     NQLEENEALALRLAENARSRGYARAAASFEEKATAARERAALLRRALLREDAGETA"
     gene            complement(1394..>2776)
                     /locus_tag="VFU72_11730"
     CDS             complement(1394..>2776)
                     /locus_tag="VFU72_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424204.1"
                     /translation="ERMHRQGQIDRLLELNREVTDANRQLTRFIDELRARNEELMLMT
                     EEAQASTEEVETLNEELQASNEELETLNEELQATVEELNTTNDELQARSIEAQHMAQV
                     SELGRARLEMILRSMADAVLVIDRTGQVELANEAARQLFGGPGREVGMLGNVALLDQD
                     GLALVGAEMLQQFAAHGESFALEFTIRTPEGEFRFYDATGGPIRSGGEGEGEVVVVRD
                     ITDRSQRQMQDEFLALANHELRAPLTVLQSSIQMLIRQLSRRPEDVGALRARADTALT
                     QAQQLGRLISDLASTTRLQSGRYQLEPAPLRLDDLVAQAVEGVRTSGEGPRIELVRGE
                     PLTVNADAGRLEQVVLNLLQNALTYAAGSERIVVRLGQAEGWARIEVQDFGSGIPAAE
                     IPRIFNRFYQVHRTDRAARGGLGLGLYISQGIARAHGGRIEVASVEGHGTTFTVLLPL
                     GSPDEREDSA"
BASE COUNT          404 a          995 c          910 g          467 t
ORIGIN      
        1 gcccatttcc tcaagccggg cggcaccttc tgcctcgtcg aagggcaccc cttcgcgcac
       61 gtcttccacg gcgagccggg cgtgctggag gtgcgctatc cctacttcga gcagccggag
      121 ccgctgcgct tcgagacgaa aggaagctac ggcgaccccg agtccgacta cgaatccgtc
      181 gagtactact ggagccactc gctgagcgag atcgtcaacg cgctgctcgg cgccgggctc
      241 gcgttggtcc acctgcgcga gttcgacttc ctctcctggc agatgggcac gaacatggaa
      301 cagggcgccg atggctactg gcgcctcccg gccggctacc cgccgctccc cttcatgttc
      361 gcgctgaaag cggtcaagcc ggggtagcga ggtttccgtt aggcggtctc gcccgcgtcc
      421 tcacgcagga gcgcccggcg cagcagggcc gctcgctccc gggcggcggt cgccttctcc
      481 tcgaagctgg ccgccgcccg tgcgtagcct cgcgaccgag cgttctcggc gagcctcaga
      541 gccagcgcct cgttctcctc caactggttg atcgcggccc acaactgctg ctcgatctgc
      601 tccgagcgag cctcgatcat gtcgtccgtc aggaaggtgt gaccgacccg gcagcgatag
      661 cggaccagct cgccctcgtg cagctcccag atcgggccgg cgcactccgg acaggtgagc
      721 gaggtcagcc tgccgaactg gttcatatcg aaggtctcta gctctcccat catttgcctg
      781 tgctccaacc gtaacatctc cggaaccgga tacgcgtctt cgcggggcac cggactgtgc
      841 gccaactcgg ccaggagtcc gccaagtgct cgggcgggca ggcgatagtc cacctcgacg
      901 taggccaggg cgctctcggg catgctgggg aagatggcgt ccgcggggtc ttggaccacg
      961 gtgacaccgc cgcgctgggc gatcgcgcgc aggccggcgg tgccgtcgcc atccatcccc
     1021 gagagcacca cacccaccac gcgcgggccg taggcgatgg ccgcgctgcg gaagagtggg
     1081 tccacggccg ggcggtgccg gttctccttc ggcccccagg tgagccgcat ccgctcacgc
     1141 tcgacgatga gatgccgatc gggcggggcc acgtagatgc gccccggtac gatcggttcc
     1201 aggtcgcgcg cctgcgccgc gggcagggtt ccggcccgct gcaggatctc cggcaggtag
     1261 ctggtgctgg tggggggaaa gtggaggacc acgaagagcg cggcgggcag atcgaacggc
     1321 agtccctcga ccaggcggat cagggcctcg acgccgcccg ccgaggcgcc gatcgtgatg
     1381 atgtcgtgcc cgctcatgcg ctgtcttctc gctcgtccgg actgcccagc ggtaggagaa
     1441 cggtgaaggt cgtgccgtga ccctccaccg aggccacctc gatgcggccg ccgtgcgccc
     1501 gcgcgatccc ctgcgagatg tacagaccga ggcccagacc gccccgcgcc gctcgatccg
     1561 tgcgatgaac ctgatagaag cggttgaaga tacgcggtat ctccgcggcc ggaatgccgg
     1621 agccgaagtc ctggacctcg atccgggccc agccctcggc ctggcccaac cggacgacga
     1681 tccgctcgct gccggcggcg taggtcagtg cgttctgcag caggttgagg accacttgct
     1741 ccagccggcc cgcgtcggcg ttcacggtca atggttcacc gcgcaccagc tcgatgcgcg
     1801 gcccctcgcc ggacgtccgc accccctcca ccgcctgcgc gaccagatca tccagccgca
     1861 gcggcgccgg ctccagttga taccggccgc tctgcagccg cgtcgtactg gccaggtcgg
     1921 agatcagccg acccaactgc tgcgcctggg tgagcgcggt atcggcgcgc gcgcgaagcg
     1981 cgccgacatc ctcgggccgg cggctgagtt gccggatcag catttgaatg gagctctgca
     2041 agaccgtgag cggggcgcgc aactcgtggt tggcgagcgc caggaactcg tcctgcatct
     2101 ggcgctggct gcggtcggta atatcgcgca cgaccaccac ctcgccttcg ccctctccgc
     2161 cgctcctgat cggacccccg gtcgcatcgt agaagcggaa ttcgccctcg ggggtacgga
     2221 tggtgaactc gagcgcgaag ctctcgccgt gcgccgcgaa ttgctggagc atctccgcgc
     2281 cgaccagcgc cagtccgtcc tgatcgagca gggcgacgtt ccccagcatg ccaacttccc
     2341 gccccggccc gccgaagagc tgccgggcgg cctcgttggc cagctccacc tgccccgtgc
     2401 ggtcgatgac cagcaccgcg tcggccatgc tgcgcaggat catctccagg cgagcgcgcc
     2461 cgagctcgct gacctgggcc atgtgttgcg cttcgatgga gcgcgcttgc agctcgtcgt
     2521 tggtggtgtt cagctcctcg accgtcgcct gcagctcctc gttgagcgtc tccagctcct
     2581 cgttgctggc ctgcaactcc tcgttgagcg tctccacctc ctcggtagac gcctgcgcct
     2641 cctccgtcat cagcatgagt tcttcgttgc gggcgcgcag ctcatcgatg aagcgggtca
     2701 actgccggtt ggcgtcggtg acctcgcggt tcagttcgag caggcggtcg atctgtccct
     2761 gccggtgcat gcgctc
//
LOCUS       DASUBI010000608         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_65285, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000608 DASUBI010000000
VERSION     DASUBI010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65285"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2920)
                     /locus_tag="VFU72_11735"
     CDS             complement(<1..2920)
                     /locus_tag="VFU72_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5424205.1"
                     /translation="MQSQSGGATGALIAPGTTQTMAWRLVGPHRGGRVVAVAGDPSEP
                     STFYFGACAGGVWKTTDGGGYWENVSDGFFTTSAIGALAVAQSDPNVLYAGTGEACLR
                     GNVSHGDGVYKSTDGGRTWQNMGLADTRHIGDIAVHPQNPDIVYVAALGHAFGPNEER
                     GVYRSTDGGKSWQKVLYKSPNAGAVDLSMDPHNPRIIYATIWQVRRNFWSLESGGPEC
                     GLYKSSDGGDTWQELTRNEGLPMGVLGKIGVAVSPAQAGRVYAIVEANDGALFRSDDG
                     GQKWQRLSEQPGLRWRAWYYMHIFADPLDANTVWVLNGGCWRSIDGGKSFSEVPTPHG
                     DNHGLWIDPKNPLRMIEGNDGGACVSYNGGRSWSTLYNQPTAQFYHVTTDTRIPYRLY
                     GSQQDNTAISLPSMSVNGAITETEWFEPGGGESGYIAVRPDDPNIVYAGAIGSGEGNG
                     RLLRYDHRTGHIRNITVWPVVQGMGEGAASLKYRFQWTFPIILSPHDPNVLYVTSNHV
                     HRTTDEGFSWETISPDLTRNDPSKQQPSGGPITKDNTGAEVYDTIFAFAVSPHEPGVF
                     WAGSDDGLVHISRDDGKSWQNITPPDLSEWALISIIELSPHQAGAAYLAATRYKLDDP
                     TPYLYKTTDYGQSWQKITEGIPATDFTRVIREDPGKRGLLYAGTETGVYVSFDDGGHW
                     QRFQLNLPVAPIHDLMLQGSDLLAATHGRSFWILDDVTPLRQMAESSGDGLTLYSPRE
                     TTRFVVYKGYGSESATEVSYRMAGPVNTAFTKEETPIGGTREDFLDAGQNPPHGVIVT
                     YAFAQAPEGEVTLTFLDAQGQEIRTFSSEKPKKAEEGPAPASESMPDDAEQGLIEGGE
                     LPEDEEKKEPRVAKDAGLNRFVWDLRYPGPTKVPGDKSTESYQEGPKVVPGSYGVRLS
                     AGGQNREASFQIVEDPRVAATQQDLQAQFDLLLQIRDKLSRTHEAINTIRNLSSQAGE
                     W"
BASE COUNT          423 a         1033 c         1154 g          572 t
ORIGIN      
        1 gccactcgcc cgcctggctg ctcaggttac ggatggtgtt gatcgcctcg tgggtgcgcg
       61 agagcttgtc gcggatctgc agcagcaggt cgaactgggc ctgcaagtcc tgctgcgtgg
      121 ccgcgacgcg cgggtcctcg acgatctgga agctcgcctc gcggttctgg ccgccggccg
      181 agagccgcac gccgtagctg cccggcacca ccttcggccc ctcctggtag gactcggtgc
      241 tcttgtcgcc gggcaccttg gtcgggccgg ggtagcgcag atcccagacg aagcggttca
      301 gcccggcgtc tttggcgacg cgcggctcct tcttctcctc gtcctcgggc agctcgccgc
      361 cctcgatcaa gccctgctcc gcgtcgtcgg gcatcgactc agacgcgggc gccggcccct
      421 cctccgcctt cttcggcttc tcgctggaga aggtgcgaat ctcctgaccc tgcgcgtcca
      481 ggaaggtcag cgtgacctcg ccctccggcg cctgggcaaa ggcgtaggtc acgatcacgc
      541 cgtgcggcgg gttctggccg gcgtcgagga agtcctcgcg cgtgccgccg atcggcgtct
      601 cctccttggt gaaggcagtg ttgaccgggc cggccatgcg gtagctgacc tcggtggccg
      661 actccgagcc gtagcccttg tagaccacga agcgggtcgt ctcgcgcggg ctgtagaggg
      721 tcaggccgtc gccggaactc tcggccatct gccgcagcgg ggtgacatcg tccaggatcc
      781 agaaggagcg accgtgggtg gccgccagca gatcgctgcc ctgcagcatc aggtcgtgga
      841 tcggggcgac cggcaggttg agctggaagc gctgccagtg accgccgtcg tcgaaggaga
      901 cgtagacgcc ggtctcggtg ccggcataga gcaggccgcg cttgccgggg tcttcgcgga
      961 tcacgcgggt gaagtcggtg gccgggatgc cctcggtgat cttctgccag ctctggccgt
     1021 agtcggtcgt cttgtagagg tagggcgtgg gatcgtcgag cttgtagcgc gtggcggcga
     1081 ggtaggccgc gcccgcctgg tgcggcgaga gctcgattat gctgatcagc gcccactccg
     1141 acaggtcggg cggggtgatg ttctgccagc tcttcccgtc gtcgcggctg atgtggacca
     1201 ggccgtcgtc ggagccggcc cagaagacgc ccggctcgtg cggtgagacc gcgaaggcga
     1261 agatggtgtc gtagacctcg gccccggtgt tgtccttggt gatcggcccg ccggagggct
     1321 gctgcttgct cgggtcgttg cgcgtcaggt cggggctgat cgtctcccag ctgaagccct
     1381 cgtcggtcgt gcgatgcacg tggttggagg tgacgtagag cacgttgggg tcgtgcggcg
     1441 agaggatgat cgggaaggtc cactggaagc ggtacttcag gctggccgcg ccctcgccca
     1501 tcccctgcac cactggccag acggtgatgt tgcggatgtg cccggtgcgg tggtcgtagc
     1561 gcagcaggcg cccgttaccc tcgccgctgc cgatggcccc ggcgtagacg atgttggggt
     1621 cgtcggggcg cacggcgatg tagccgctct cgccgccgcc cggctcgaac cactcggtct
     1681 cggtgatggc gccattgacc gacatgctcg gcagcgagat ggcggtgttg tcctgctgcg
     1741 agccgtagag ccggtagggg atccgggtgt cggtggtcac gtggtagaac tgcgcggtcg
     1801 gctggttgta gagcgtcgac caactgcgcc cgccgttgta gctgacgcag gcgccgccgt
     1861 cgttcccctc gatcatgcgc agcgggttct tggggtcgat ccagaggccg tggttgtcgc
     1921 cgtgcggcgt cggcacctcg ctgaagctct tgccgccgtc gatcgagcgc cagcagccgc
     1981 cgttgagcac ccagacggtg ttggcgtcga gcgggtcggc gaagatgtgc atgtaatacc
     2041 aggcgcgcca gcgcagcccg ggctgctccg agagccgctg ccacttctgg ccgccgtcgt
     2101 cggagcggaa gagggcgccg tcgttggcct cgacgatggc gtagacccgc cccgcctggg
     2161 ccggcgagac ggccacgccg atcttcccca gcacgcccat gggcaggccc tcgttgcgcg
     2221 tcagctcctg ccaggtgtcg ccgccgtcgc tcgacttgta gaggccgcac tcgggaccgc
     2281 cgctctccag gctccagaag ttgcggcgca cctgccagat ggtggcgtag atgatacgcg
     2341 ggttgtgtgg gtccatggag agatcgaccg cgccggcgtt cgggctcttg tagagcacct
     2401 tctgccagct cttcccgccg tcggtcgagc ggtagacgcc acgttcctcg ttggggccga
     2461 acgcgtggcc cagcgcggcc acgtagacga tgtccgggtt ctgggggtga acggcgatgt
     2521 cgccgatgtg gcgcgtatcg gccaggccca tgttctgcca ggtcctgccg ccgtcggtcg
     2581 acttgtagac gccgtcgccg tggctgacgt tgccgcgcag gcaggcctcg ccggtgccgg
     2641 cgtagagcac gttggggtcc gactgggcca cggccagggc gccgatggcg ctggtggtga
     2701 agaagccgtc ggagacgttc tcccagtagc cgccgccgtc ggtggtcttc cagaccccgc
     2761 cggcgcaggc gccgaagtag aaggtcgagg gctcgctggg atccccggcg accgcgacca
     2821 cgcgcccgcc gcggtgcggc ccgaccaggc gccaggccat ggtctgcgtc gtgcccggcg
     2881 cgatcaaggc gccggtggcg ccgcccgatt gactctgcat ggtgtcgttc ctcccaggta
     2941 cgaggctcat ctcatcgcgc ggcagtgtcg catgtccgcc gggaggtgga aacctgtccg
     3001 gggttgccct catcccccgc ccccttctcc caatgctggg agaaggggag tcatagggga
     3061 aagaaagtgc catacgaagg cgatgggtgt tgtccaagag gctgcttgct taccccgtca
     3121 tcctgagcgg agtcgaagga tccgtgctga cgcggcactc aacgcagtcg ggcggcttcg
     3181 aa
//
LOCUS       DASUBI010000609         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1134066, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000609 DASUBI010000000
VERSION     DASUBI010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134066"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="radA"
                     /locus_tag="VFU72_11740"
     CDS             <1..1078
                     /gene="radA"
                     /locus_tag="VFU72_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5424206.1"
                     /translation="GGSPGIGKSTLTSMALGNLQGAGRKTLYVSGEESAAQIRLRAER
                     LPGAALEVPVLAETDLDTVLATLDAERPEVCVVDSVQTLHAQDLTGAAGSVGQVREVA
                     DRITRLAKQRDIAVILVGHVTKEGALAGPRVLEHLVDCVLQFEGERERTYRTLRALKN
                     RFGSTNDVGVFEMREGGLVEVQDASARFVGEATRAPGSVVLAAMEGSRPLLVEVQALV
                     SPSELVPPRRVVNGIDRNRLALVLAVLGRHAGIGAGSADVFVNVAGGVRVEEPGADLA
                     VALAVAGAVKNVTLGPSEKVPLACFGEIGLTGELRAVAHADRRLAEARKFGLDPVLQP
                     GPETPTLRSAVRAALTAPPSRVAA"
     gene            1167..>2087
                     /gene="disA"
                     /locus_tag="VFU72_11745"
     CDS             1167..>2087
                     /gene="disA"
                     /locus_tag="VFU72_11745"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEU5424207.1"
                     /translation="MQPRSGEELTELEARQEPRLVKALEMVAPGTALREGMDNIVHAR
                     TGGLIVIGDPDELAFLFSGGIRLDIDYTPALVYQLAKMDGAIALNANSTKIAWCNVQL
                     MPDPTILTSETGTRHRTAERISKQTDALVIAVSQRRDVVSLYVDGAKYILEEIPAVLA
                     KANQALATLDKYRSRLDQVSTRLTALEFEGGVTLHDVLTVLQRAELVTRMAVEIERYI
                     VELGNEGRLIEMQLEETMVGVAGDKAALLHDYLAEPVDDAFAIALDGVARLPHQDLLD
                     FGRLAELLGYDRKVNTLDYPVSPRGYRILGR"
BASE COUNT          307 a          720 c          756 g          304 t
ORIGIN      
        1 cggcggctct cccggcatcg gcaagtcgac gctcacctcg atggcgctgg ggaacctcca
       61 gggcgcgggg cgcaagacgc tctacgtgtc cggcgaggag tccgcggcgc agatccggct
      121 gcgggccgag cggctgcccg gcgcggcgct ggaggtgccg gtgctggcgg agaccgacct
      181 ggacacggtg ctggccacgc tcgacgcgga gcggcccgag gtgtgcgtgg tcgactcggt
      241 gcagacgctg cacgcccagg acctcaccgg cgccgccggg agcgtcggcc aggtgcggga
      301 ggtcgccgac cgcatcacgc ggctggcgaa acagcgcgac atcgcggtca tcctcgtcgg
      361 ccacgtcacc aaggagggcg cgctcgccgg cccccgggtg ctcgagcacc tggtcgactg
      421 cgtgctgcag ttcgagggcg agcgggagcg gacctatcgc acgctgcggg cgctgaagaa
      481 ccgcttcggc tcgaccaacg acgtcggcgt cttcgagatg cgcgagggcg gcctggtgga
      541 ggtccaggac gcctcggcgc gcttcgtggg cgaggccacg cgggcgcccg ggagcgtggt
      601 gctcgccgcc atggagggct cgcggccgct gctggtggag gtccaggcgc tcgtcagccc
      661 gtcggagctc gtgccgccgc ggcgggtggt caacgggatc gaccgcaacc ggctcgcgct
      721 cgtgctcgcg gtcctgggcc gccacgcggg catcggggcc ggcagtgcgg atgtgttcgt
      781 gaatgtggcc ggcggcgtgc gcgtggagga gccgggcgcc gatctcgcgg tggcgctcgc
      841 ggtggccggc gcggtcaaga acgtgacgct ggggccgtcc gaaaaggtgc cgctcgcctg
      901 cttcggggag atcgggctca ccggtgagct gcgcgccgtg gcccacgccg accgacggct
      961 tgcggaggcc cggaagttcg gtctggaccc cgtgctgcag cccggtccgg agacgccgac
     1021 gctgcgttcc gcggtgcgcg cggcactcac cgcaccacct tcccgcgtag cagcctgaag
     1081 cggtttcgtc aagccccatt tttggctcta tagctgggct ttcgcatcgt aaatggcgct
     1141 taagcaactg ctagaatctg ttcgtgatgc agccgcgatc aggtgaagag ctcactgagc
     1201 tcgaagcgcg gcaagagccc cggttggtca aagcgctgga gatggtcgcg cccggcaccg
     1261 cgttgcggga gggcatggac aacatcgtcc acgcgcgcac cggcggcctg atcgtgatcg
     1321 gcgatccgga cgagctcgca ttcctgttct ccgggggcat ccggctggac atcgactaca
     1381 cgccggcgct cgtctaccag ctggccaaga tggacggggc gatcgcgctg aacgccaact
     1441 ccaccaagat cgcctggtgc aacgtccagc tgatgccgga cccgaccatc ctcacgtcgg
     1501 agaccggcac acgtcatcgc acagccgagc gcatctccaa gcagacggat gcgctggtga
     1561 tcgccgtctc ccagcggcgc gacgtcgtct cgctctacgt cgacggcgcg aagtacatcc
     1621 tggaggagat ccccgccgtc ctggccaagg ccaaccaggc gctggccacg ctggacaagt
     1681 accgcagccg gctcgaccag gtctccaccc gcctgaccgc gctggagttc gagggcggcg
     1741 tgacgctgca cgacgtgctg accgtgctcc agcgggccga gctggtgacc cgcatggccg
     1801 tcgagatcga gcgctacatc gtcgagctgg gcaacgaggg ccggctcatc gagatgcagc
     1861 tcgaggagac catggtcggc gtggccggcg acaaggccgc gctgctgcac gactacctgg
     1921 cggagcccgt cgacgacgcg ttcgcgatcg cgctggacgg cgtcgcccgg ctcccgcacc
     1981 aggacctgct cgacttcggg cgcctggccg agctgctcgg ctacgaccgc aaggtcaaca
     2041 cgctcgacta tcccgtctcg ccccgcggct accgcatcct cggccgc
//
LOCUS       DASUBI010000610         5652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_999671, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000610 DASUBI010000000
VERSION     DASUBI010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999671"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFU72_11750"
     CDS             <1..1336
                     /locus_tag="VFU72_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424208.1"
                     /translation="QTLRAALAWSYDLLTLAEQHVFARLAVMTGDCDLEAAEAVCREP
                     DLPAGSVLDLLTRLVDKSLLLAEAGVTSARYRLLEPVRQYALRLLEADGTLEQVRERH
                     AAYFLALAEQAAGELRGPEQIAWLDRLESEHDNLRAALLWSLADGDRVLGLRLAVALG
                     PFWGVRGRLREGRDWLDRLLNAPGAGELPERLRVRALLADGRLAQWQGDFEAADSLLT
                     TCIELAHQLGEPLIAAEALVHLGAVYRRRGDPEGSARLLDESLHAFRAAGDEGGIALA
                     LMTLGVTLRSLGETARSTELLEESLARFQQLGDLRWIATTQTMLGSSLLKQGKLTGAA
                     RSFVAGLAAHQAIGDLTFSVFALHGLGAALARLAALEPATQVYGVMLALCDRVGLTLD
                     PVSQRDYDSVTAGLRAQLGAADFDRLLAEGRTLSPDQVLDGVSRVLGDLSAE"
     gene            2088..2795
                     /locus_tag="VFU72_11755"
     CDS             2088..2795
                     /locus_tag="VFU72_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477240.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEU5424209.1"
                     /translation="MAGLIASLLGIILINVVLSGDNALVIGMAARNLQPRQRRWAILA
                     GGAAAIVLRVIFTFVAVLLLRVPLLEALGGLLLVWIAYKLLSEGEEAEVVGSESILGA
                     VWTIVFADMVMSLDNMLAVAGAANGSDWLVFFGLVLSMPLILFGATLIARLLNRFSWL
                     MIVGSAVLTITAANMVVSDEIVGKAVEGWHELALAGLAAGFTLLVLAPTLVRWRRLRL
                     ERLEERVRHPDRVHLPE"
     gene            2823..3089
                     /locus_tag="VFU72_11760"
     CDS             2823..3089
                     /locus_tag="VFU72_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424210.1"
                     /translation="MEPFTLDDLYRIYIIVPREDGLGTENLTVAQMSDRQFRWWVKEK
                     SAREGVRMILPIGQIRHQTRLYMLNRLRDSGVKIYKLTEPQPGS"
     gene            complement(3228..4142)
                     /locus_tag="VFU72_11765"
     CDS             complement(3228..4142)
                     /locus_tag="VFU72_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5424211.1"
                     /translation="MLRSAMLALAGNDWVSQRVTHADLTRDLALRFVAGDSFEEAAEV
                     VRRLNRRGLTVTLDKLGENVASAEAAGAAAAEYRTILQRIKAEGVDSAISVKLTMLGL
                     DIDEQLCRANLRPILETARELGTFVRIDMEGSAYTARTLAIFYDLHAEFGDHVGIVLQ
                     SYLYRTEQDVATAIERGARVRLVKGAYLEPPAVAYPRKSDVDAAYRREMEQLLEHGNY
                     PAIATHDEAILRVARGFTRRLGIGQDRFEFQMLYGIRRDLQQQLTGDGYRLRIYVPFG
                     AQWYPYFMRRMAERPANLLFVLRNLTRR"
     gene            4578..>5652
                     /locus_tag="VFU72_11770"
     CDS             4578..>5652
                     /locus_tag="VFU72_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5424212.1"
                     /translation="MAEAWVTTIRDSLYDRIPLTSADVALIETPAFLRLDRIQQLGFV
                     SKVWPGAKHTRFEHSLGVFHLMRQALAGLRARPEGTLIDDTTARAALAAALLHDVGHY
                     PFSHGIEELGAPVPPHEAVGRSVIERGEVAAVLAERWAVDPARVAAFIDPRNGLSDID
                     RLVRGLLSGALDVDKLDYLPRDARNCNVPYGGVDTPRLLDALRVGTVDGSPRLVLGEK
                     GVSPLASLIHARQEMFDNVYWHHTNRACMVMLLRAVQDALLAGRLEPAALAGHDDCSL
                     QEALAAPEMPASTRALVDGLRGRKLHKRAVEVSARAGELYRYLGGLYAEPARRRAVEL
                     AIAARLGRELGTEVADHEVLIDIP"
BASE COUNT          785 a         1870 c         2092 g          905 t
ORIGIN      
        1 gcagacgctg cgggcagcgc tggcctggag ctacgatctg ctgacgctgg ccgagcagca
       61 cgtcttcgcc cggctggcgg tgatgaccgg cgattgtgac ctggaggccg ctgaggcggt
      121 ctgccgtgag cccgatctgc ccgccggaag cgtgctcgat ctgctgacac gcctggtcga
      181 caagtcgctg ctgctggccg aggcgggcgt cacgagcgcg cgctaccgct tgctggagcc
      241 ggtgcggcag tacgccttgc gcctcctcga ggcggacggc acgctggagc aggtgcgcga
      301 gcggcatgcc gcgtacttcc tggcgctggc cgagcaggcg gctggtgagc ttcgcggccc
      361 cgagcagatc gcctggctgg accggctgga gagtgagcac gacaatctgc gtgcggcgct
      421 cctgtggtcg ctggcggatg gcgatcgcgt gcttgggctg cggctggcgg tggcgctcgg
      481 gccattctgg ggcgtgcgtg gccggctgcg tgaagggcgc gattggctcg atcgactgct
      541 caacgcgcct ggagcgggcg agctgcccga gcggctgcgg gtgcgggcgc tgctggctga
      601 cgggcggctg gcgcagtggc agggcgattt cgaggcggcg gactcgctgc tgacgacctg
      661 catcgagctg gcgcaccagt tgggggaacc gctcatcgcc gccgaggcgc tggtccacct
      721 gggcgcggtc taccggcgac gcggcgaccc cgaaggctcc gcgcggctgc tggacgagag
      781 cctgcacgcg tttcgcgcgg ccggcgatga ggggggcatc gcgctggcgc tgatgacgct
      841 cggcgtcacg ctgcgctcgt tgggggagac cgcccgctcg acggagctcc tcgaggagag
      901 cctggctcgc tttcagcagc ttggtgatct gcgttggatc gccacgacgc agaccatgct
      961 ggggtcatcc ctgttgaagc aggggaagct gacgggcgcc gcgcgctcgt tcgtggccgg
     1021 attggcggcc caccaggcca tcggcgatct caccttcagc gtctttgcgc tgcatggcct
     1081 gggggccgcg ctggcacggc tggctgccct ggagccggcg acgcaggtgt acggcgtgat
     1141 gctggcgctg tgcgaccggg tgggattgac gctcgacccc gtgtcgcagc gcgactacga
     1201 cagcgtcacc gccggcctgc gcgcccaact cggcgcggct gacttcgacc ggctgctggc
     1261 cgagggccgc acgctgtcgc cggatcaggt gctggacggc gtctcccgcg tgctcggcga
     1321 tctcagcgcg gagtgaggcg gacggcgggc gcacgacgac gacatgcctc gtccggtagt
     1381 cctcttctcc tcccctgtca tcctgagctt gtcgaaggat ccgtcgtaga gcggcgccca
     1441 acgtcgtcgc tggccgcgtc ccgtcgcccg agctcgcgcg ggctacgaga acggcctgtc
     1501 gacgtattgc ggcgtgtgag gacggatcct tcgacaagct caggatgaca gggctcatta
     1561 cgggcatacg acgacgattg gtgtcgtcag ctacgccccg attcctcccc cataggctcc
     1621 cctctcccag cattggctct gtcaggagag gggttggggt ctccgtgcgt gtggcccggc
     1681 tactgccgca ggtagcgcag ggccgccgtg gtcagcacct cggccacgcc cattaggatc
     1741 gtcgtgtggg catcgtggcc gcaggcgtgc aatcacgcca ttgccgccgg tggtgaggcc
     1801 aggggtgatg ccggcggcgc gcggccaggc gtccagcccg ggcgtcacca gctccgcgac
     1861 tcggcctgcc tcagtcacgg gttccccctc atgcggtcac ctatctcacg tgcgcatcgc
     1921 gagctatccg cgcgggatcg catgagacgc gcgccgtgag cttcttcttt cgcctcggac
     1981 gacgatggta cgatgatcgc tagaccccgg ggtaggcgct gcgcgggcgc ggctccgccc
     2041 cggcgcgcgc cgggtctatc gagtgctgaa acacgtgttg ggaatccatg gccggactga
     2101 tcgccagtct tctcgggatc atcttgatca acgtcgtgct cagcggcgac aacgccctgg
     2161 tcatcggcat ggcggcgcgc aacctccagc cgcgccagcg gcgctgggcc atcctggccg
     2221 gcggggcggc cgccatcgtg ctgcgggtga tcttcacctt cgtggcggtg ctgctgctgc
     2281 gggtgccgct gctggaggcg ctcggcgggc tgctgctggt ctggatcgcc tacaagctgc
     2341 tctcggaggg cgaggaggcc gaggtcgtcg ggagcgagag catcctggga gcggtctgga
     2401 cgatcgtctt cgccgacatg gtgatgagcc tggacaacat gctggcggtg gcgggcgcgg
     2461 ccaacggcag cgactggctg gtcttcttcg gcctggtgct ctcgatgccg ctgatcctct
     2521 tcggcgcgac cttgatcgcg cggctgctca accgcttctc ctggctgatg atcgtcggct
     2581 cggcggtgct gaccatcacc gcggccaaca tggtggtgag cgacgagatc gtcggcaagg
     2641 cggtcgaggg ctggcacgag ctggcgctgg ccgggctggc ggcgggcttc accctgctgg
     2701 tgctggcgcc gacgctcgtg cgctggcggc gcctgcgcct ggagcggctg gaggagcgcg
     2761 tgcggcaccc cgaccgggta cacttgccgg agtagcggag actggaaagg acgcgcgcgc
     2821 tcgtggagcc gttcacgctg gacgacctgt accgcatcta catcatcgtg ccgcgcgagg
     2881 atgggctcgg caccgagaac ctgacggtgg cgcagatgag cgaccggcag ttccgctggt
     2941 gggtcaagga gaagtcggcg cgtgagggcg tgcgcatgat cctgccgatc ggacagatcc
     3001 gccaccagac gcgcctctac atgctcaacc gcctgcgcga ctccggcgtc aagatctaca
     3061 agctgaccga gccgcagccg gggagctagg ggccgccctc atcccccgcc cttctcccga
     3121 cgcgcgggag aagggagaag aaggaactcc tgtaggggca gacccctgtg tctgccctcg
     3181 ttgttttgcg cgccggctgg gggaaagaat acgcgccgcg cctgtcccta ccgccgcgtc
     3241 aggttgcgca ggacgaagag gaggttggcg gggcgctcgg ccatgcggcg catgaagtag
     3301 gggtaccact gcgcgccgaa ggggacgtag atgcgcaggc ggtagccgtc gccggtgagc
     3361 tgctgctgca gatcgcgccg gatgccgtag agcatctgga actcgaagcg gtcctggccg
     3421 atgcccagcc gccgggtgaa gccccgcgcc acgcgcagga tcgcctcgtc gtgggtggcg
     3481 atggccgggt agttgccgtg ctccagcaac tgctccatct cgcggcggta ggcggcgtcg
     3541 acgtcggact tgcgcgggta ggcgacggcc ggaggctcca ggtaggcgcc cttgaccagg
     3601 cgcacccgtg ccccgcgctc gatggcggtc gcgacatcct gctcggtgcg gtagaggtac
     3661 gactgcagca cgatgccgac gtggtcgccg aactcggcgt gcaggtcgta gaagatggcg
     3721 agggtacgcg cggtgtaggc cgagccctcc atgtcgatgc gcacgaaggt gcccagctcg
     3781 cgggcggtct ccaggatcgg gcgcaggttg gcgcggcaga gctgctcgtc gatgtcgagg
     3841 ccgagcatgg tcagcttgac cgagatggcg ctgtcgaccc cctcggcctt gatccgctgc
     3901 aagatcgtgc ggtactcggc cgcggcggcg ccggcggcct cggcgctggc gacgttctcg
     3961 ccgagcttgt cgagggtgac ggtcaggccg cgccggttga ggcggcgcac gacctcggcg
     4021 gcctcctcga agctgtcgcc ggccacgaag cgcagcgcta gatcgcgcgt gagatcggca
     4081 tgggtcacgc gctgggagac ccaatcgttt ccggccagag ccagcatcgc ggagcggagc
     4141 atcggcctct tccccgggtg acgacggcgg aacggcggtt atggggcatg ccgcggcttc
     4201 cgagttggct aggagccgcg tagcgcccgg gaatcaaatt cccgggctga atccacgaag
     4261 cccactgaag gggctgggag ttggtatcgt cacgttgttg cgtcttgccg gcgtgtgcac
     4321 atcaccatgt tcggcgcgaa ggttccatcg ccgccaagcc taagttcagc ccctttagtg
     4381 ggcttcgtgg actcagcgcg gtggctctag cccggcgcga cgagcgcggc gattgtccac
     4441 gggatgcggc gaccccagca accgcgcacc ctaccacccc ccagtgtagc acgcgcgtct
     4501 caccgccacg cgaacatcag ttcggataga attggcgcgt agcgtgcgcg cccgccgtat
     4561 gcccaaagga gccgccgatg gccgaagcgt gggtcaccac catccgcgac agcctctacg
     4621 accgcattcc gctgacttca gccgacgtgg cgctgatcga gacgcccgcc ttcctgcggc
     4681 tcgaccgcat ccagcagctg ggcttcgtca gcaaggtctg gccgggggcc aagcacaccc
     4741 gcttcgagca ctcgctgggg gtcttccacc tgatgcgcca ggcgctggcc gggctacgcg
     4801 cccggcccga gggcacgctc atcgacgaca ccaccgcccg cgccgcgctg gccgccgcgc
     4861 tgctgcacga cgtgggccac taccccttca gccacggtat cgaggagctg ggcgcgccgg
     4921 tgccgccgca cgaggccgtc gggcgaagcg tcatcgagcg cggcgaggtg gcggcggtgc
     4981 tggcggagcg ctgggcagtg gacccggcgc gcgtggccgc cttcatcgat ccgcgcaatg
     5041 gtctctccga catcgaccgg ctggtgcgcg ggctgctcag cggcgcgctg gatgtcgaca
     5101 agctcgacta cctgccgcgc gacgcgcgca actgcaacgt gccctacggc ggggtggaca
     5161 cgccgcgcct actcgacgcg ctgcgggtgg gcacggtcga cggcagcccg cgcctcgtgc
     5221 tcggcgagaa gggggtcagc ccgctcgcct cgctgatcca cgcccgccag gagatgttcg
     5281 acaacgtcta ctggcaccac accaaccgcg cctgcatggt gatgctgctg cgcgcggtgc
     5341 aggacgcgct gctggccggg cggctggagc cggccgcgct ggccggccac gacgactgct
     5401 cgctgcagga ggcgctggcc gcgcccgaga tgccggccag cacgcgggcg ctggtcgatg
     5461 ggctgcgcgg aaggaaattg cacaagcgcg cggtcgaggt cagcgcgcgg gccggagagc
     5521 tgtatcggta tctcggcggg ctctacgccg agccggcgcg gcggcgggcg gtggagctgg
     5581 ccatcgcggc gcggctgggg cgcgagctgg ggacggaggt ggccgaccac gaggtgctga
     5641 tcgacatccc ca
//
LOCUS       DASUBI010000611         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_740017, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000611 DASUBI010000000
VERSION     DASUBI010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740017"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1145
                     /locus_tag="VFU72_11775"
     CDS             174..1145
                     /locus_tag="VFU72_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424213.1"
                     /translation="MNIGRTFIREQSTATVGEYRRRVYRLALIYLGLLVVTIATIVII
                     VVNSHFFVLLTQRSNVETAVLLFIIVLFAYLGTISAPGVWGALRIVSYDAPRWLGRDP
                     LPAERRKQQALKKTSGNSKSVYLNQIVALREQPDAPIVIPIADAAGGLGELHIDGARM
                     VHHTPFKVGSNSMFAFFAQRIQALINQHPWDVPIEIVQWTAIDDEQALQYGSLVSFAQ
                     NLRRQLQGAPLWPTVELSQGDLDCLIAEASALCPALRNEGYLPDVEYRAEHKLPIIPE
                     PLAFISLSRSEQRADPAASMGCALLMAFLILALFTFLVLVPPWVPPR"
     gene            1142..1483
                     /locus_tag="VFU72_11780"
     CDS             1142..1483
                     /locus_tag="VFU72_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424214.1"
                     /translation="MIGRTAVVLAAALALLALAACGGPRPVVDGVELRPPQQDGQPYL
                     LVVTLRNASGGQGQVTVSARITGANGQVAASEEQHTASLSPHEVERLTIDVQPAAPGP
                     YTVEVKASYPP"
     gene            complement(1489..2232)
                     /locus_tag="VFU72_11785"
     CDS             complement(1489..2232)
                     /locus_tag="VFU72_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5424215.1"
                     /translation="MAADTEDYPALPPPYAEALREAVAYLRERFTPSAIIASGTIVRG
                     DPGPSSDLDLYVIIERPERQRLQRFFHGVPAEIFVNSLNWIERYFGAEIEAGRPVTAH
                     MLATGVAVYDPSGTGAALIARATGLLAEGPRPSEATLVSVRYYAATNLEDGFDILESG
                     PELARAFLYRAVEGALRYRFLSAGQWQPRFKELLGALDQLDPKLAELTRRFYRASVLD
                     EQATLAREIVARTVGETGFFPWESAVEPE"
BASE COUNT          478 a         1067 c          994 g          439 t
ORIGIN      
        1 ttgccgccga tgccgtgcag cagcaccagc ggtggcccgc tccccgcgcg acggtaggcg
       61 atgcgccgcc catcgactat gacggtctgc cgcgtatcca tgcagcacac tcctctcgca
      121 ttcaggcctg gaacctgcga tagcctgccg gcagtggagg gttggcgggt cgggtgaaca
      181 tcgggcgcac gttcattcgc gagcagagca ccgccaccgt cggcgagtac cggcggcggg
      241 tctaccgcct ggccctcatc tacctcgggc tgctggtcgt gaccatcgcc accatcgtaa
      301 tcatcgtcgt caacagccac ttcttcgtgc tgctcaccca gcgctccaac gtcgagaccg
      361 ccgtcctcct cttcatcatc gtgctcttcg cctacctcgg caccatcagc gcgccggggg
      421 tctggggcgc gctgcgcatc gtctcctacg acgcgccgcg ctggctgggt cgcgacccac
      481 ttcccgccga gcgccgcaag cagcaggcgc tcaagaagac atccgggaac agcaagtcgg
      541 tctatctcaa tcaaatcgtc gcgctgcggg agcaaccgga cgcgccgatc gtcatcccga
      601 tcgcggacgc ggccggcggc ctgggcgagc tccacatcga cggcgcccgc atggtgcacc
      661 acacgccctt caaggtcggc tcgaacagca tgttcgcctt cttcgcccag cggattcagg
      721 cgctgatcaa ccagcacccc tgggacgtgc cgatcgagat cgtgcagtgg accgccatcg
      781 acgacgagca ggcgctgcag tacggcagcc tggtcagctt cgcccagaat ctgcgccggc
      841 agctccaggg cgcgccgctc tggccgaccg tcgaactcag ccagggcgac ctcgactgtc
      901 tgatcgcgga ggccagcgcg ctctgtccgg ccctgcgcaa cgagggctac ctgccggacg
      961 tcgagtaccg cgccgagcac aagctaccga tcatccccga gccgctggcc ttcatctcgc
     1021 tctcgcgcag cgagcagcgc gccgatcccg cggcctcgat gggctgcgcc ctcctgatgg
     1081 cgttcctcat cctggctctc ttcaccttcc tagtgctggt gccgccctgg gtgccgccgc
     1141 gatgatcggc cggactgccg tcgtcctcgc cgccgcgctg gcgctgctgg cactcgctgc
     1201 ctgcggcgga ccgcggccgg tggtcgacgg cgtcgaactg cgtccgcccc agcaggacgg
     1261 ccagccctac ctgctggtgg ttacgctgcg caacgctagc ggcggccagg ggcaggtgac
     1321 ggtctcggcg cgcatcaccg gggccaacgg acaagtggcg gccagcgaag agcagcacac
     1381 cgcctcgctc tcgccgcacg aggtcgagcg cctgaccatc gacgtgcagc cggccgcgcc
     1441 cggcccctac acggtcgagg tcaaagccag ctacccgccg tagcgcgcct actccggctc
     1501 gacggccgat tcccagggga agaagccggt ctcgcccacc gtgcgcgcca cgatctcgcg
     1561 cgccagggtg gcctgctcgt cgaggacgga cgcacggtag aagcggcgcg tgagctccgc
     1621 cagcttcgga tcgagttggt cgagcgcccc cagcagctcc ttgaagcgcg gctgccactg
     1681 gcccgcgctg aggaagcgat agcgcagcgc gccctcgacc gctcggtaga ggaaggcacg
     1741 agccagctcc ggcccgctct ccaggatatc gaatccgtct tccaggttgg tggcggcgta
     1801 gtagcgcacg ctgacaagcg tggcttcgga cgggcgcggg ccctcggcca gtagcccagt
     1861 cgcgcgggcg atcagcgcgg cgccggtgcc cgagggatcg tagacggcga caccggtcgc
     1921 cagcatgtgc gccgtgaccg ggcggcccgc ctcgatttca gcgccgaaat agcgctcgat
     1981 ccagttcaag gaattgacga agatctcggc gggcacgccg tggaagaagc gctgcagccg
     2041 ctggcgctcg ggccgttcga tgatgacgta gaggtcgagg tcgctgctgg ggccggggtc
     2101 gccgcgcacg atcgtgccgc tggcgatgat cgcgctgggg gtgaaacgct cgcgcaggta
     2161 cgccaccgcc tcgcgcagcg cctcggcgta gggaggcggc agggcgggat agtcctctgt
     2221 gtccgcggcc atcactccct cgtctcaaag cggcgcagtc cgtcctgtta tcctgaggcc
     2281 gccaccgaag gatctctcct cggccccaag cagccacacg gctcatgcga caggccaacc
     2341 gcagaatcag cagaacggac tcagcccctt cagtgggctt cgtggagtta gcgcggtggc
     2401 tttagccaga cggagacggc gcaccggcgg gcacccacgt tcccgactac atcttcaacg
     2461 tccgtcaatc ccgggcggca ggcggcacga tggtcacacc gaaggcgtgg agagagagga
     2521 ccgcgtccgt gacccgacag gagggcgggg tgaaagccag gccacggact ctggagtagt
     2581 cagaggcggt acgtgatcgg cgccccggtg gcgcctgctc acgcggggag tactcgggga
     2641 gtgaccctct gaggggtgca aagagcacaa gccgtgccac cacttcccct gccccgcgag
     2701 cggcacgcgc gatgcgtacc cacgcccgtc gcccgcgcgt cactcgcaca cgcgcgaccg
     2761 cgcggcagtg aggcagtgag gcaggcggag ccgagccggg agaggcatgt ggcaggcaag
     2821 cggcgtcagt ccgcgcaacc ggcgaagcgt acgcgtgagc ctggacgtgg gcgtgactgg
     2881 ctcgcctacg tcaaggccct cgggccaggg atcatctccg gtgcctcgga caacgacccc
     2941 acgaccgtcg ccaccatggc cgtgatcggc gcctccac
//
LOCUS       DASUBI010000612         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1180005, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000612 DASUBI010000000
VERSION     DASUBI010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180005"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFU72_11790"
     CDS             <1..351
                     /locus_tag="VFU72_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5424216.1"
                     /translation="EPRAYRPALSPGEATEQLRAEVRAGRLDRAAVAAVLSAAGQQAG
                     PLAGAWPAGLRDREVDVLRLIIRGLPNRAVAQQLNISAKTVGHHVEHIYAKIGVSTRA
                     GAALFAMEHGLLQG"
     gene            469..1302
                     /locus_tag="VFU72_11795"
     CDS             469..1302
                     /locus_tag="VFU72_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424217.1"
                     /translation="MTASTIRVNDVDLAYVTQGDGVPVIFVHGGLGDYRNWDAQMGPF
                     AERYRAVAYSRRYHWPNARPGEGVHYRAAQHAADLGALIEALGLAPAHVVGSSYGALT
                     ALILATARPELFRSLVLGEPPLLLWLARLPGGQPLFDAFMANAFGPAREAFARGEAEV
                     GVRRFFDGALGPGVFDRFPAPARQQRLDNAAEMRVETATPSEEYFSALSPEDVARLQT
                     PTLLLEGALSPTMFGLMTDELARVLPHAERATIPAASHGMHGQNPPAYNAAVLAFLAE
                     H"
     gene            complement(1321..2133)
                     /locus_tag="VFU72_11800"
     CDS             complement(1321..2133)
                     /locus_tag="VFU72_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5424218.1"
                     /translation="MAAPEVGSSHPEPLSGAAAYEPYVGRWSRLVARDFLAWLAIPAG
                     ARWLDVGCGTGALSASILAQAAPREVVGIEPLAERVAYAREQVRDRRARFDVGDAQAL
                     PYESGSFDAAVSGLVLNFVPDPRRALAELARVTRAGGTVAAYVWDYAGEMQLMRHFWD
                     AAVALDPNALELDEGRRFPLCRPGPLGELFRGAGLREVEARAIDVPTVFRNFDDYWTP
                     FLGGQGPAPGYAVALDEERRATLRERVRARLPIQPDGSIQLIARAWAARGTR"
     gene            complement(2268..>2633)
                     /locus_tag="VFU72_11805"
     CDS             complement(2268..>2633)
                     /locus_tag="VFU72_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424219.1"
                     /translation="ELPQLREAVAQPVIKELDLHAAGITTVIWATGYSFDFSLVKLPV
                     FERDGFPLQQSGVTAYPGLSFVGMPWMPSLKMGTLMGVGESAARIVARLTSQAGVRAS
                     GIGETLAVEPRPARAAAGA"
BASE COUNT          378 a          993 c          916 g          346 t
ORIGIN      
        1 gagccgcgcg cctaccgccc cgcgctgtcg ccgggcgagg ccaccgagca gctgcgcgcc
       61 gaggtacgcg ccggccggct cgaccgcgcc gcggtggcgg ccgtgctgag cgccgccggc
      121 cagcaggctg ggccgctggc cggcgcctgg cccgccgggc tgcgcgaccg cgaggtcgat
      181 gtcctgcgcc tgatcatccg cggcctgccc aaccgggcag tcgcccagca gctcaatatc
      241 tcggccaaga cggtcggtca tcacgtcgag cacatctacg ccaagatcgg ggtctccacc
      301 cgcgccgggg ccgcgctctt cgccatggaa cacggcctgt tacaaggctg agaccggggt
      361 ccgaaatagg gcgatcaccc gatgccgcgc gtcaccccag ggcctacgct cctcctgtca
      421 accacggttg actgagcgca cgagtaagtc agcggaagga gcgcagcgat gacagcatcg
      481 acgatccggg tcaacgatgt cgacctcgcc tacgtcacgc agggtgacgg cgtgccggtg
      541 atcttcgtcc acgggggcct gggcgactac cgcaattggg acgcgcagat ggggccgttc
      601 gcggaacgct accgggccgt cgcctacagc cggcgctatc actggcccaa cgcgcggccg
      661 ggcgaggggg tccactacag agccgcccag cacgccgccg acctgggcgc gctgatcgag
      721 gcgctcggcc tggcacccgc ccacgtcgtc ggctcctcct acggcgcgct gacggcgctg
      781 atcctggcga ccgcgcgacc ggagctattc cgctcgctgg tgctgggcga gccgccgctc
      841 ctcctgtggc tggcgcgcct gcccggtggg cagcccctct tcgacgcgtt catggcgaat
      901 gccttcgggc cggcgcgcga ggccttcgcg cgcggcgagg cggaagtcgg cgtgcggcgc
      961 ttcttcgacg gcgcgctcgg cccgggtgtg ttcgaccgct tcccggcgcc ggcccgccag
     1021 cagcgtctgg acaacgccgc cgagatgcgg gtcgagacgg ccacaccgtc cgaggaatac
     1081 ttctccgcgc tctcccccga ggacgtcgcg cgcctccaga cgcccacgct cctgctcgag
     1141 ggagcgctca gcccgaccat gttcgggttg atgaccgacg agctggcgcg cgtgctgccg
     1201 cacgccgagc gcgcgaccat ccccgccgcc tcgcacggca tgcacggcca gaacccgcca
     1261 gcctacaacg cggccgtgct cgccttcctg gcggaacact gagcttccac gccggcgcgg
     1321 ctagcgtgtg ccgcgtgccg cccaggcgcg ggcgatgagc tggatcgagc cgtcgggctg
     1381 gatggggagg cgggcgcgga cgcgctcgcg cagcgtggcc cggcgctctt cgtcgagcgc
     1441 cacggcgtag cctggggccg ggccctgacc gcccaggaac ggggtccagt agtcgtcgaa
     1501 gtttcggaag accgtgggca cgtcgatggc gcgcgcctcg acctcgcgca gaccggcgcc
     1561 gcggaagagc tctcccagcg ggcccggccg gcagagcggg aagcggcgtc cctcgtccag
     1621 ttccagcgcg ttggggtcga gcgccaccgc cgcatcccag aagtggcgca tcagttgcat
     1681 ctcgccggca tagtcccaga cgtaagccgc gactgtcccc ccggcgcgcg tcacccgcgc
     1741 cagctcggcc agcgcccgcc gcggatcggg cacgaagttc agcaccagcc cggagacggc
     1801 cgcgtcgaag ctgcccgatt cgtagggcag cgcctgggca tcccccacgt cgaagcgggc
     1861 gcgccggtcg cgcacctgct cgcgcgcgta ggccacgcgc tcggccagcg gctcgatccc
     1921 caccacctcg cgcggggcag cctgagccag gattgaggcg ctgagcgcgc cggtcccgca
     1981 gccgacatcg agccagcggg cgccggccgg gatcgccagc caggccagaa agtcgcgcgc
     2041 gaccaggcgg ctccagcgcc ccacgtaggg ctcgtacgcc gccgcgccgc tcaatggctc
     2101 cgggtgactc gagcctacct ccggtgctgc catcgcccgc gatcctctct agagatggtc
     2161 caggacgcct gagctgaatc gacacgcaac cgcgggacgc aatactaccg aaaaggggcc
     2221 gccgatgcgg ccccacttgc accccgattg cctgccgtcg ccgggcctca ggcgccggct
     2281 gccgctctgg cgggacgcgg ctcgacggcc agcgtctccc caataccgga ggcgcgcacc
     2341 cctgcctggc tggtgaggcg cgctacgatg cgcgcggccg attcgcccac gcccatcagc
     2401 gttcccatct tcagcgacgg catccagggc atcccgacga aggagagacc cgggtaggcg
     2461 gtcacgccgc tctgctgcag cgggaagccg tcgcgctcga agaccggcag cttcaccagg
     2521 ctgaagtcga agctgtagcc ggtcgcccag atgacggtcg tgataccggc ggcgtgcaga
     2581 tcgagctcct tgatcacggg ctgcgcgacg gcttcgcgca gctggggcag ctc
//
LOCUS       DASUBI010000613         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1067292, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000613 DASUBI010000000
VERSION     DASUBI010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067292"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..886)
                     /locus_tag="VFU72_11810"
     CDS             complement(101..886)
                     /locus_tag="VFU72_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5424220.1"
                     /translation="MAITVDFSGEVALVTGAATGLGLAIAEALGAAGATVALTDLSRE
                     RAESAVAALRERDIAAHGWAADVRDAPAMARVLEEIGARLGRLDVAVASAGIYPNTPL
                     LELTPDEWDRVLDTNLKGVFITCQAAARLMIAGGRGGRLVTLSSGAANHAIWGWAHYS
                     ASKAGVVALIRALALELAPHGIRANAVLPGYIDVPEGGRPLSESYKETARRGIPLRRG
                     EPADIANAVVMLASPLAGFVTGTALSVDGGSSAGRAQLRPVEE"
     gene            complement(892..1758)
                     /locus_tag="VFU72_11815"
     CDS             complement(892..1758)
                     /locus_tag="VFU72_11815"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEU5424221.1"
                     /translation="MDALRGTVCLTFDFDAISLWIGPYKATSPSKISRGEFGVVGVER
                     ILTVLQRAGLPATFFVTGHTADTYPSSVRAIKDAGHEIGHHGYLHENPVTLERVEERI
                     ILEKGLAALERAAGVRPTGYRSPAWDNSPNTIELLEEFGFRYESSLMGQDFRPYWART
                     RDVIQRDGAYQFGPESNIVELPVSWILDDFPHFEYVSSENRVSPGLSAPSKVEEIWRG
                     EFDFMVREVPGGVITLTMHPQVIGRGHRILMLERLIDYFKEHEGVRFATLGQVADEFR
                     ASHPRTQAKAEG"
     gene            complement(1987..3906)
                     /locus_tag="VFU72_11820"
     CDS             complement(1987..3906)
                     /locus_tag="VFU72_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5424222.1"
                     /translation="MADNTFEALLAAARARRLTRRQVLVRGAALGLSAPAIAALLAAC
                     GGSSKTATSAATTATAAPTTAGSGAASPASTTAATTAATTATPSATTSAATPAAATRG
                     GAGAITILWWQAPVILNSHLSVAGKDVGAQRIVLEPLADFDKNARLVPVLAAEIPSVE
                     NGGVAKDGTSVTWKLRQGVKWHDGQPFTANDVAFTFAYLNDPATAATTRGFYQDVATV
                     DAVDDYTAKVTFKHPTAPWFNPFTAIAGVILPQHILSDFVGAKAKSAPFNLKPIGTGP
                     FKVTEFKPGDVVSYSLNPDYWDPGKPHFDSVTLKGGGDATSAARAVMQSGEADWAWNL
                     QVEATVLKSLESAGRGKLVIWPGAGTEKMIINHSDPNTEVDGQKSSYKTEHPHFKVKE
                     VRQAVNLAIDRDSMATQLYGATGKATGYTMNENPTYMPSGITWKYDLKAAADLLDKAG
                     AQKGSDGIRSLNGRKLSWLYTASTNSLRQKEQEIIKQSLGQIGVDIEIKAVDASAYFD
                     ATNEASFQHLYCDIGMERNAAGVYPLLWYLRYLSVDPSRDIAQKENNWGGRNIQRYQN
                     PQFNQMYQQVSQEIDPAKYTQMFKDMQTLVVEDVADIGLVSGNNVAAASNKLTGYDPS
                     PYATDTWDIKNWTKA"
     gene            complement(3972..4595)
                     /locus_tag="VFU72_11825"
     CDS             complement(3972..4595)
                     /locus_tag="VFU72_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU5424223.1"
                     /translation="MDDYLARLARLDTAGVADAQKGMGILEPAIRSMTPGRKLAGPAF
                     TVKCYPGSIITVHKAFVEARPGDVLVVDGEGDLRAGALIGELMSREAVERGFAGVVID
                     GPVRDLTGLRALGFPTFARGVTPRVGVNRRVGQTQVPVSAGAVLVRPGDYVLADDDGV
                     AIIPHEDLVAIVEAVEAIERKEAGLAEAIARQERLVDLLNFREAIKG"
     gene            complement(4689..5285)
                     /locus_tag="VFU72_11830"
     CDS             complement(4689..5285)
                     /locus_tag="VFU72_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5424224.1"
                     /translation="MSQGIGEREPNGHGPAVEELDLGRRIRATRHERGFSLAELAARA
                     EVSKSLISQIERGIVAPSVETVRKLASALEVPVFSLFLSSLEGDLVVRAHERRAVSYP
                     GSPILREILSPNLKGRMVLLWATLPPQAESGPIPVHHTGEESVVVIRGALEVLVGERS
                     TRLASGDAITFDSELPHSFRNPGDEPCEAVVAISPPHL"
BASE COUNT          794 a         1876 c         1955 g          933 t
ORIGIN      
        1 cttcagtggg cttcgcggac tcagcccgag aatcgaattc tcgggcgacc agcgggcacc
       61 tactcaccag atcagccgcc cgatcgtcct gctccccgcc tcactcctcg accggccgca
      121 gctgcgcgcg cccggcgctg ctgccgccgt cgacggagag cgccgtgccg gtgacgaagc
      181 cggccagcgg cgaggcgagc ataacgaccg cgttggcgat gtcggccggc tcgccgcgcc
      241 gcagcgggat gccccggcgg gcagtctcct tgtacgactc gctcagcggg cggccgccct
      301 cgggcacgtc gatatagccg ggcaagacgg cgttggcgcg gatgccgtgg ggcgccagtt
      361 ccagcgccag cgcacggatc agcgccacca cacccgcctt cgaggcgctg tagtgcgccc
      421 aaccccagat ggcgtggttg gccgcgcccg agctgagcgt cacgaggcgg ccaccgcgcc
      481 cgcccgcgat catcaggcgc gcggcggctt ggcaggtaat gaagacgccc ttgaggttgg
      541 tgtcgagcac gcggtcccac tcgtccggcg tcagctccag cagcggcgtg ttgggataga
      601 tgccggcgct ggcgaccgcg acgtcgagcc gccccagccg cgcaccgatc tcctccagca
      661 cgcgcgccat ggccggggcg tcgcgcacat ccgccgccca gccgtgggcc gcgatgtcgc
      721 gctcgcgcag cgccgccacg gcggattcgg cccgctcccg gctcaggtcg gtcagcgcga
      781 cggtcgctcc agccgcgccc agcgcctcgg cgatggccaa accgagtccg gtggcggcac
      841 cggtcacgag cgcgacctcg ccggagaaat cgacggtaat cgccatgccg gctacccttc
      901 cgctttcgct tgtgtccgag ggtggctggc gcggaactcg tcggccacct ggcccaaggt
      961 ggcgaagcgc acgccctcgt gctccttgaa gtagtcgatc agccgctcca gcatcaggat
     1021 gcggtggccg cgcccgatca cctgcgggtg catcgtcagg gtgatcacgc cgcccggcac
     1081 ctcgcggacc atgaagtcga actcgccgcg ccagatctcc tcgaccttgg agggggccga
     1141 gaggccgggg ctgacccggt tctcgctgga gacgtactcg aagtgcggga agtcgtcgag
     1201 gatccagctc accggcagct cgacgatgtt cgactccgga ccgaactggt acgcgccgtc
     1261 gcgctggatc acatcgcgcg tgcgtgccca gtagggccgg aaatcctgcc ccatcagcga
     1321 gctctcgtag cggaagccga actcctccag cagctcgatg gtgttggggc tgttgtccca
     1381 ggcgggcgag cggtagccgg tggggcgcac cccggcggcg cgctccagcg cggcgagccc
     1441 cttctccagg atgattcgct cctcgacccg ctccagcgtc accgggttct cgtgcagata
     1501 gccgtggtgg ccgatctcgt gaccggcatc cttgatggcg cgcaccgatg atggataggt
     1561 gtcggcggtg tggccggtga cgaagaaggt ggcggggagg ccggcgcgct ggagtaccgt
     1621 caggatgcgc tcgaccccga ctaccccgaa ttcgccgcgc gagatcttgc tgggcgaggt
     1681 ggccttgtag ggaccgatcc agagcgagat ggcgtcgaaa tcaaaggtca ggcaaaccgt
     1741 gccccgtagc gcgtccatgg cgtgctcccc tccgtcgcgg cgtcggcgat cgtcggcggc
     1801 agtgtcggac cggggcgcgg cggcgtcaac ggggacggcc ctcgtcccgg acggacatcc
     1861 cgccccctgc tcccagcatt gggagcaggg ggaacgaagg tgggcgggtt tcaaacccgc
     1921 ccctacaacg tttcgtcgtg ctcccctcgc ccagcattgg gagaggggcc gggggtgagg
     1981 gcgcgtctac gccttcgtcc agttcttgat atcccaggtg tcggtggcgt acgggctggg
     2041 gtcgtaaccg gttagcttgt tgctggcagc cgcgacgttg ttgcccgaga ccaggccgat
     2101 gtcggctacg tcctcgacca ccagcgtctg catgtccttg aacatctggg tgtacttggc
     2161 ggggtcgatc tcctgcgaga cctgctggta catctggttg aactgcgggt tctggtagcg
     2221 ctggatgttg cgcccgcccc agttgttctc cttctgggcg atgtcccgcg aggggtcgac
     2281 cgagaggtag cgcagatacc agagcagcgg gtagacgccg gccgcgttgc gctccatgcc
     2341 gatgtcgcag tagagatgct ggaacgaggc ctcgttggtg gcatcgaagt aggccgaggc
     2401 gtcgaccgcc ttgatctcga tatcgacgcc gatctggccc agtgactgct tgatgatctc
     2461 ctgctccttc tggcgcaggg agttcgtcga cgcggtgtag agccaggaca gcttgcgccc
     2521 gttcaggctg cggatgccgt ccgagccctt ctgagccccg gccttgtcga gcaggtccgc
     2581 ggcggccttg aggtcgtact tccaggtgat gccgctcggc atgtaggttg ggttctcgtt
     2641 catggtgtag ccggtggcct tgcccgtcgc gccgtagagc tgcgtcgcca tcgagtcgcg
     2701 atcgatcgcg agattgacgg cctggcgcac ctctttcact ttgaagtgcg ggtgctccgt
     2761 cttgtaggac gacttctggc cgtcgacctc ggtattcggg tcggagtgat tgatgatcat
     2821 cttctcggtg cccgcacccg gccagatgac cagcttgcct cgcccggccg actccagcga
     2881 cttgagcacg gtcgcttcga cctggagatt ccaggcccag tcggcctcgc cggactgcat
     2941 cacggcgcgc gccgcgctgg tggcgtcgcc gccgcctttg agcgtgacac tgtcgaaatg
     3001 cggcttgccg gggtcccagt aatcggggtt gaggctgtag ctgaccacgt cgcctggctt
     3061 gaactccgtc accttgaagg gcccggtgcc gatcggcttg aggttgaagg gcgcgctctt
     3121 ggccttggcg ccgacgaagt cgctcaggat gtgctggggg aggatcacac ccgcgatcgc
     3181 ggtaaagggg ttgaaccagg gcgcggtggg atgcttgaag gtgaccttgg ccgtgtagtc
     3241 gtcgacggcg tccacggtcg cgacatcctg gtagaagccg cgggtggtgg cggcggtcgc
     3301 cgggtcgttg aggtaggcga aggtgaaggc cacatcgttg gcggtaaacg gctggccgtc
     3361 gtgccatttg acgccttgcc gcagcttcca ggtgacgctg gtgccgtctt tggcgacgcc
     3421 gccgttctcg acgcttggaa tctcggcggc taggaccggc acgagcctgg cgttcttgtc
     3481 gaaatcggcc agcggctcga gcacgatgcg ctgcgcgccg acgtccttgc cggcgaccga
     3541 gagatgcgag ttgaggatga ctggcgcctg ccaccagagg atggtgatcg ccccggctcc
     3601 gccgcgcgtc gcggcagcgg gcgtggctgc cgacgtggtc gcggacggtg tggccgtcgt
     3661 cgccgccgtg gtggcggcgg ttgtgctcgc cgggctggcc gcgccgctgc cggccgtggt
     3721 gggtgccgcc gtcgccgtgg tcgccgcgct cgtcgccgtc ttgctgctgc ccccgcaggc
     3781 cgccagcagg gccgcgatgg cgggggcgct caacccgagc gccgcaccac gcaccagtac
     3841 ctggcgccgg gtgagccgcc gcgcgcgggc cgcggccagc agtgcctcga aggtgttgtc
     3901 agccatgtgc gtctccttgc tggagtggtc cagcggcctg gttctcggcg ccggctggac
     3961 tgacgctacc cctacccctt gatcgcctcg cggaagttga gcaggtcgac cagacgctcc
     4021 tggcgcgcga tggcctcggc cagaccggcc tccttgcgct cgatcgcctc gaccgcctcc
     4081 acaattgcca ccaggtcctc atgcgggatg atcgccacac cgtcgtcgtc ggccagcacg
     4141 tagtcgccgg gacgcaccag cactgcgccg gcgctgaccg gcacctgcgt ctggcccacc
     4201 cggcggttga cgccgacgcg cggggtgacg ccgcgggcga aggtggggaa gccgagcgcg
     4261 cgcaggccgg tgaggtcgcg cacgggcccg tcgatgacca cgccggcgaa gccgcgctcg
     4321 accgcctcgc gcgacatcag ctcgccgatc agcgcgccgg cccgcagatc accctcgccg
     4381 tccacgacca gcacatcccc cggccgcgcc tccacgaacg ccttgtgtac ggtgatgatg
     4441 ctgccggggt agcacttgac ggtgaaggcc gggccggcca gcttgcggcc cggcgtcatg
     4501 ctccggatcg ccggctccag gatacccatg cccttctgcg cgtcggccac cccggcggtg
     4561 tccaggcgcg ccaggcgcgc caggtagtcg tccacctcgc cctccatcca tgaccgtcaa
     4621 ccgaatatgg ttcaacctta gtggacgaag cgtgcagccg tcaagtggag ctgaacaacc
     4681 gcaagcggct agagatgggg cggggagatg gcgaccacgg cctcgcaggg ctcgtcgccg
     4741 gggttgcgga aggagtgggg cagctcggaa tcgaaggtga tcgcgtcgcc cgaggcaagc
     4801 cgggtggagc gctcgccgac cagcacctcg agcgcgccgc ggatcacgac cacgctctcc
     4861 tcgccggtgt ggtggaccgg aatcgggccg gactcggcct gtggcggcag ggtggcccag
     4921 agcaggacca tgcgtccctt gaggttggga gagaggatct cgcgaaggat cgggctgccg
     4981 gggtagctga ccgcgcggcg ctcgtgggcg cgcacgacca ggtcgccctc caggctgctg
     5041 agaaagagcg agaagacggg cacctccagc gccgaggcga gcttgcgcac cgtctcgacc
     5101 gacggtgcca cgatgccgcg ctcgatctgg ctgatcaggc tcttgctgac ctcggcgcgc
     5161 gccgccagct cggccagcga gaagccgcgc tcgtgccgcg tggcgcggat tcgccggccc
     5221 aggtccagct cctccaccgc cgggccgtgt ccgttcggct cccgctctcc gatcccctgg
     5281 ctcatgccgc ttcgccctcg cattcacgtc cgctgcacgc gccagccggc gcgtacagtg
     5341 aggctatcat tcagattcgt cttttgtcaa agcgagtgcc cgagcaggtt tggagcagcg
     5401 tacgaggcca tcgtcatctg gccaataccg tagcgcggag tccggccagc ccgccggggg
     5461 caatcaagtt gtcgacgatc cggtggtctg ggacggattt catccggcta gacgaatggc
     5521 cgtttcctgg cctggagccg aaggaagagg tggcgcgc
//
LOCUS       DASUBI010000614         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_658374, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000614 DASUBI010000000
VERSION     DASUBI010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658374"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..405)
                     /locus_tag="VFU72_11835"
     CDS             complement(247..405)
                     /locus_tag="VFU72_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424225.1"
                     /translation="MLNLGPQPESFALIDPAGSGTIVLSTHLDRENQRVESELALRPN
                     EGLILALD"
     gene            complement(439..1848)
                     /locus_tag="VFU72_11840"
     CDS             complement(439..1848)
                     /locus_tag="VFU72_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5424226.1"
                     /translation="MSGERQDYRWWQTGIIYQIYPRSYQDSDGDGIGDLPGITCRLDY
                     LQWLGVDALWLSPIFPSPMADFGYDVADYTAIDPIFGSMADFDRLLAEAHQRGLKVIL
                     DYVPNHTSSQHPWFLESRAARDSPKRDWYLWRDPAPGGGPPNNWLSVFGGGAWEWDEP
                     TGQYYYHAFLKEQPDLNWRNPEVRRAMFDVLRFWLDKGVDGFRIDVIWHLIKDDRFRD
                     NPANPHAVPGYEYSELVPVFTSDQPEVFEVIGAMRRVFDEYDDRVMIGEIYLPPERLV
                     TYYGARGDLVQLPYNFQLILLPWDARELAVAIDEYEALLPEGAWPNWVLGNHDRQRIA
                     SRVGPEQARVAAMLLLTLRGTPTIYYGDEIGMHDVPILPEQVRDPHELREPGIGAGRD
                     PERTPMQWDGKPNAGFTSGTPWLPLAEDAGQLNVEIEREERHSMLALQRELIELRRRA
                     PALVTGGYTPVPASDDLLA"
     gene            complement(1964..2926)
                     /locus_tag="VFU72_11845"
     CDS             complement(1964..2926)
                     /locus_tag="VFU72_11845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5424227.1"
                     /translation="MLPYADEPAVTKRSFPIVNVLLIALNIVVFLYELDLGQNGAERF
                     ITRWGFIPADFWAGAGLVTIVTALFIHAGLLHIGGNMLYLWVFGDNVEDQLGHITYLI
                     FYLLAGIVGNIGFAVAFAHTGQPLVGASGAIAGVLGGYLLLYPRAIVRTLLTFGPFIT
                     LGGVAAVIVIGFWFLLQVFESLVSILAVSPSAADSGVAFFAHVSGFIFGLVVTGIIRE
                     ARDQQLTHWHRERWWSRSFRNWALLAIVATVVGIAGQLIIGHSGAVVRTVAGGALVAI
                     ALLDGIGRLRGNPALLGTVPRSHKLIAILQILAALSLAGAVIGT"
     gene            3135..>5009
                     /locus_tag="VFU72_11850"
     CDS             3135..>5009
                     /locus_tag="VFU72_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424228.1"
                     /translation="MTAEHASPYLFVSYASADQERVLPLVERLEAAGVHTWVDREGIH
                     GGANYAQVINDAIKGAAALALMASPASLASRNVRQELALGWRYETPYLPLLLDSVEIP
                     DELAYWLEGSQWIELLDRPVQDWLGDVVRALEALGLSVQLQPETAAAARRPRPLLVGR
                     EREQGILRQQLDRLLAGQGGVVLVGGEAGIGKSTLVEDLSAQAEDAGALVLWGHAYDL
                     SVTPPYGPWLEIFAAYQPPVQGLPPLPAFVGSAEELAKVGSQERLFAVVVAFFQAVAA
                     QRPLVLVLDDLHWADEASLDLFRVLARQVAGHRLLLVADYRSDELRRGHPLHSLLPLL
                     VREAGAERLEVRPLRAIDYRTLIQVRYALPDDDRARLERYLAARAEGNPLYAGEVLRT
                     LEEEGVLVQRDGAWRLGDLEQARVPTLLRQIIEGRVGRFDEESRVLLSVAAVVGQTVP
                     LGVWAAAGEVDEDALLELAERAEEARLLEATPEGAGVRFPHALLREALYEGIPTIRRR
                     RLHRRVGELLAAGATPDPDMVADHFIRAADSRAADWLVKAGERAEQAYDWQAAVKRLE
                     QAIGIMEGDPQRRREVAQLLYQMGDLDRYGDPARSVSYLDRSARLARESGDDALALMA
                     E"
BASE COUNT          747 a         1666 c         1822 g          774 t
ORIGIN      
        1 cgtgtcttct ccaggttgta gcggaccatg gccacggtca cgccgttctc ggtgagcttg
       61 ccgatgccct tgggccgctc tactaccgtt cgtcgccgtt gctccatctg tgctgctccc
      121 cgccgactgc cccgtacgta cacgagagtt tagcacagat gttcgtgcct gaaaaggctt
      181 ctcacggctg ttaatgcacg gccgtctatc gcgacattac cgcgtacgtt cgcacggtct
      241 gctggctcaa tcgagggcca ggatcaggcc ctcgttgggg cgcaatgcca gctcgctttc
      301 gacgcgctgg ttctcccggt cgaggtgggt cgagaggacg atggtgcccg atccggccgg
      361 gtcgatcagc gcgaaggact cgggctgcgg gccgagattg agcacgatca gcaggcgcgt
      421 accgttctgc tcgcgaattt aggccagcag gtcgtcgctg gccggtaccg gcgtgtagcc
      481 gccggtgacc agcgccgggg cgcggcggcg tagttcgatc agctcgcgct gcagcgccag
      541 catcgaatgc cgctcctcgc gctcgatctc gacgttgagc tgcccggcgt cctcggcgag
      601 cggcagccag ggggtgccgc tggtgaagcc ggcgttcggc ttgccgtccc actgcatcgg
      661 ggtgcgctcg gggtcgcgcc cggcgccgat gcccggctcg cgcagctcgt gcgggtcgcg
      721 cacctgctcg ggcaggatcg gcacgtcgtg catgccgatc tcgtcgccgt agtagatggt
      781 cggggtgccg cgcagggtca agaggagcat ggcggccacg cgcgcctgct ccgggccgac
      841 ccggctggcg atgcgctggc ggtcgtggtt gccgagcacc cagttgggcc aggcgccctc
      901 gggcaacagc gcctcgtact cgtcgatggc caccgccagc tcgcgcgcgt cccaggggag
      961 caggatcagc tggaagttgt aagggagctg caccaggtcg ccgcgcgcgc cgtagtaggt
     1021 taccaggcgc tcgggcggca ggtagatctc gccgatcatc acgcggtcgt cgtactcgtc
     1081 gaagacccgg cgcatcgcgc cgatcacctc gaagacctcc ggctggtcgc tcgtgaagac
     1141 cggcacgagc tcgctgtatt catagcccgg caccgcgtgc gggttggccg ggttgtcgcg
     1201 gaagcggtcg tccttgatca gatgccagat gacgtcgatg cggaagccgt cgaccccctt
     1261 gtcgagccag aagcgcagca cgtcgaacat ggcccggcgc acctcggggt tgcgccagtt
     1321 gaggtcgggc tgctccttga ggaaggcgtg gtagtagtac tgcccggtcg gctcgtccca
     1381 ctcccaggcg ccgccgccga agaccgagag ccagttgttg ggcgggccgc cgccgggggc
     1441 ggggtcgcgc cagagatacc agtcgcgctt ggggctgtcg cgcgcggcgc gcgactccag
     1501 gaaccagggg tgctggctcg aagtatggtt aggcacgtag tccagaatga ccttcagccc
     1561 gcgttgatgc gcttcggcca gcaggcggtc gaagtcggcc atgctgccga agatggggtc
     1621 gatggcggtg tagtcggcca cgtcgtagcc gaagtcggcc atcggcgagg ggaagatcgg
     1681 cgagagccag agggcgtcga cgccgagcca ctgcaggtag tcgaggcggc aggtgatgcc
     1741 cggcaggtca ccgataccat cgccgtcgct gtcctgatac gagcgcgggt agatctggta
     1801 gatgatcccc gtctgccacc agcgatagtc ctgccgctcg ccgctcatcg tcgctccctc
     1861 gtcgcgcggg ccgggcgccc ggcgctcgca ccggcaccac cacgctcccg cttaccaatc
     1921 cctgtcggca ggctgctatc gcaacatccg tgcgcgcttc ccgctaggtg ccgatcaccg
     1981 cgccggccag cgagagcgcg gccaggatct gcaggatggc gatcagcttg tggctgcgcg
     2041 gcacggtgcc caagagcgcc ggattgccgc gcagccgccc gatgccgtcg agcaacgcga
     2101 tggcgaccag cgctccgccg gcgaccgtgc gcacgaccgc gccgctgtgg ccgatgatca
     2161 gctgcccggc gatccccacc acggtggcca cgatcgccag cagcgcccag ttgcggaagg
     2221 agcggctcca ccagcgctcg cggtgccagt gcgtgagctg ctggtcgcgc gcctcgcgga
     2281 tgatgccggt cacgacgaga ccgaagatga agccgctgac gtgggcgaag aaggccacgc
     2341 cgctgtcggc cgcgctcggc gagacggcca ggatcgagac cagcgactcg aagacctgga
     2401 gcaggaacca gaagccgatc acgatcacgg ccgccacgcc gccgagggtg atgaaggggc
     2461 cgaaggtgag gagggtgcgc acgatagcgc gcgggtagag caggaggtag ccgccgagca
     2521 ccccggcgat ggcgccgctg gcgccgacca ggggctggcc ggtgtgcgcg aaggcgaccg
     2581 cgaagccgat gttgcccacg atcccggcga gcagatagaa gatcaagtag gtgatgtggc
     2641 cgagctgatc ctccacgttg tcgccgaaga cccagaggta gagcatgttg ccgccgatgt
     2701 gcagcagccc ggcgtggatg aacagcgccg tgacgatcgt gaccaacccg gccccggccc
     2761 agaagtcggc ggggatgaag ccccagcgcg tgatgaagcg ctcggcgccg ttctgcccca
     2821 gatccagctc gtagaggaag acgacgatgt tgagtgcgat caggaggacg ttgacgatcg
     2881 ggaaggagcg tttggtgacc gccggctcgt cggcataggg gagcatcggc acctccggcg
     2941 ctgggacggc aatccagccc gcgcgacacg cgtgggccag cggagattgt cagggcagcg
     3001 gccgcggctg acaaccgcct ggcccggacg tgacacgacc cgcgacgcgg aatttcgtgc
     3061 ctcggtgcta tactctcctt cgttcttcac cgcgcgcagg ttcgccagac ggtttcatac
     3121 cgcatgaggg ggtcatgacc gcggaacacg cgagccccta cctcttcgtc agctacgcca
     3181 gtgccgacca ggagcgcgtg ctgccgctgg tcgagcgtct ggaagccgcc ggggtccata
     3241 cctgggtgga ccgtgagggc atccacggcg gggccaacta cgcccaggtc atcaacgacg
     3301 ccatcaaggg tgcggcggcc ctcgccctga tggcctcccc cgcctcgctc gcctcgcgca
     3361 acgtccgcca ggagctggcg ctcggctggc gctacgagac gccctacctg cccctcctgc
     3421 tcgactctgt cgagatcccg gacgagctag cctactggct ggaaggctcc cagtggatcg
     3481 agctcctcga ccgcccggtg caggactggt tgggcgatgt ggtgcgcgcg ctggaggcgc
     3541 tggggctgag cgtgcagctc caaccggaaa ccgcggcggc cgcgcgccgt cctcgcccgc
     3601 tgctggtcgg gcgtgagcgg gagcaaggca tcctgcgcca gcagctcgac cggctgctgg
     3661 ccgggcaggg cggggtcgta ctcgtcggcg gggaagcggg gatcggcaaa tccacactgg
     3721 tggaagacct gagcgcgcag gccgaggacg ccggcgcact cgtcctctgg ggccacgcct
     3781 acgacctatc agtcacgccc ccctatggcc cctggctgga gatcttcgct gcctatcagc
     3841 cacccgtgca gggcttgcca ccgctccccg ccttcgtcgg cagcgccgag gagctggcca
     3901 aggttggctc ccaggagcgg ctcttcgccg tggtcgtcgc cttcttccag gcggtcgcgg
     3961 cccagcgtcc gctggtgctg gtgctcgacg atctgcattg ggccgacgag gcgagcctcg
     4021 atctcttccg tgtactggcc cggcaggtgg cgggtcaccg cctgctcctg gtggccgact
     4081 accgctccga tgagctgcgc cgcgggcacc cgctccactc actcttgccg ctactcgtgc
     4141 gggaagcggg cgcggagcgg ctggaggtcc ggccgctgcg ggcgatcgat taccggacgc
     4201 tcatccaggt gcgctacgcg ctgccggatg acgaccgggc gcggctggag cgctatctgg
     4261 cggcgcgcgc ggaggggaat cccctctacg cgggcgaggt gctgcgcacc ctggaggagg
     4321 agggtgtgct ggtccagcgc gacggcgcct ggcggctggg cgacctggag caagcgcggg
     4381 tgcccacgct cttacggcag atcatcgaag ggcgcgtggg gcgctttgac gaggagagcc
     4441 gagtgctcct gtcggtggcc gcggtggtcg gccagacggt gccgctgggt gtctgggccg
     4501 ccgccggcga ggtcgacgag gatgcgctgc tcgagttggc cgagcgggcg gaggaggccc
     4561 gcctgctgga ggcgacaccg gagggcgcgg gcgtgcgttt cccgcacgcg ctgctgcggg
     4621 aggcgctcta cgaggggatt ccgaccatcc ggcgccggcg gctgcaccgg cgggtgggcg
     4681 agctcctcgc agcgggcgcg acgcccgatc cggatatggt ggccgaccac ttcatccggg
     4741 cggcggactc ccgtgcggcc gattggctgg tcaaggccgg ggagcgggcg gagcaggcct
     4801 acgactggca ggccgcggtc aagcggctcg agcaggcgat cggcatcatg gagggcgatc
     4861 cgcagcgacg gcgggaggtc gcccaactcc tctatcagat gggcgatctc gatcgctatg
     4921 gcgaccccgc ccgcagcgtg tcctacctcg accggtcggc gcggctcgcg cgcgagagcg
     4981 gtgatgacgc cctcgcgctg atggccgag
//
LOCUS       DASUBI010000615         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_182814, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000615 DASUBI010000000
VERSION     DASUBI010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182814"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFU72_11855"
     CDS             complement(<1..993)
                     /locus_tag="VFU72_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5424229.1"
                     /translation="MPPSLHITLLGDFSLVDGGTPITGVDTPRLQSLLAYLLLHRDAP
                     QARQQLAYLFWPDSTEKQARTNLRRELHNLRHALPEPDRYLAIGTSTLQWRPNAPYTL
                     DVDEFEAAIAAGRRAEKSTDGSSFRSSLAQAAATYRGDLLPGCYDDWITGERERLRGL
                     SIWALGQLVIALEEAHDYAAATRYAERVLTIDPLHDTACRALMRLHALNGQRASALRA
                     YRAYAARLEEEFGVEPDPDTRALYQQLLTADTAVTPPVVLPGITPLVGRVAEWGQLLR
                     AWRVSVQGHGQLALIEGEAGIGKSRLAEELRTWASRQGIATAAARSYAAEGEHVY"
     gene            1365..3602
                     /gene="mrdA"
                     /locus_tag="VFU72_11860"
     CDS             1365..3602
                     /gene="mrdA"
                     /locus_tag="VFU72_11860"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5424230.1"
                     /translation="MSRYRSPYSRQPRRRRVLPTLVVSVLGLLVIVLGLFVVASHLGA
                     TEGVTTRSGIDRTPPPTAAATVPGASPAANLAASPAASPTVNPATLKSPLDVAKAYVQ
                     LWNGGNFDGMYQLISTVAQSTISQKDFSGRYQGIDQEVGQSSIKVTINNNTPDDATHV
                     PIHVARESGKVGHLEEDNLIPLVKDGANWRIDWTPSLIFADLGDGFVRWKPDVPQRGR
                     ILDRKGRPLAHLGTISKVGIVPGEIKDEAAMLDQLSQLLNMPKDTIKSLYANGQPDWF
                     MPIHNYPDPMDPTLEQKLKAIPGIEIQKWPDRVYPDGAASAQVVGYLSQVTADELPEL
                     SKQGYSAGDMVGRAGIEAWGEKYLAGKRGGRLVIVGSDGSERKTLGEVKSEPSDDIVL
                     TIDLDLQNAAFQLLADKPGSIVVLDPNSGAVLAMASNPSFDPNQFILGLSDQQWTDLN
                     NPQTHPLQNRATQSAYPIGSTFKVVTMAAAMQSLGLTPSSTIPCPGSFTIPGGSTVWH
                     DWSLTGQGTLTLHNALVQSCDTVFYKLGADLDTKDQNLLPNMARAFGFGKPTGIELPE
                     VPGVVPDAEWKLKATGDYWARGDAVNLAIGQGYFLGTPLQVADAYAAIANGGTLWQPY
                     VVQEVHKLDGTKVYSAQPKERGKLPITADQVGAIRSALKDVVSAPNGTAVAAFKGEQH
                     PVAGKTGTAETNKPEPHSWFVSFTPVDGAKFASVTAIENAGEGGDIAAPIERQLIDKY
                     YQLYP"
     gene            complement(3681..4136)
                     /locus_tag="VFU72_11865"
     CDS             complement(3681..4136)
                     /locus_tag="VFU72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5424231.1"
                     /translation="MTLSDRDSMPKTISRRSVLRYAAAVPAAFAVGGLLAACGGGGSS
                     NGGSSGGGSDHTVNMTDALKFEPASITIKKGDSITWKNTGQLDHTATDNDKAQNPADA
                     KLPSGAQAWDSGNVGPGKTFKHTFDVAGDYTYYCIPHEAGGMVGHVTVQ"
     gene            complement(4280..>6230)
                     /locus_tag="VFU72_11870"
     CDS             complement(4280..>6230)
                     /locus_tag="VFU72_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5424232.1"
                     /translation="GLGAIVFFWMLQTGTHLFLGRLAGYAPPAPLGEGNHGASSLARP
                     WALPLVVALGGLLSGLLVFTLAPEAEGHGTDAAIDAFHHKGGRVRARVIPVKTLASAI
                     TIGTGGSAGREGPTAQISAGVGSLLGQWLGLDPNDRRILLATGIGAGIGAIFRAPLGG
                     AVLAAEILYTHDMEIEALFPGLIASIVGYTIFGAWAGWEPIFGAQPQLGFTSPVQLLY
                     YALLGLLCGVVGILYAKTFYSVAGIFRRLPLPAWLKPVIGGALVGLLGLVLPQVLGTG
                     YGWLQEGLGAPLTISLWALLLLPFAKILATSLSVGSGGSGGIFGPGMVIGGSLGWAFW
                     QVSHAMLPGMPESAAPFTIVAMAALFGGIAHAPLAVMLMVGEMTGNLSLLAPAMIAVG
                     LATLVTGEHTIYTSQLPTRADSPAHRYQFSFPLLNTLAVRDAIGKAVVLLTPRQPLAE
                     AERVLRAHRLPGAAVVDEHHQLVGVIRIDDLASVAEDQRADTTVATLVAAPPVVIDSD
                     SSLDVALELLTTNQVNWAPVVEARDRVVGVITAADIVATYRAALRWTVRRLHGITADT
                     VLLESRIAPGSPLAGKALRELHFPGNALVVSIHRSGATVLPRGSSTLEAGDLVAVLAN
                     PESETAVRDFLGGAGQTRPLSAGFS"
BASE COUNT         1024 a         2147 c         2169 g          890 t
ORIGIN      
        1 gtagacgtgc tcgccctccg cagcgtagga gcgagccgcg gcggtcgcga tcccttgccg
       61 gctggcccag gtgcgtagct cctccgccag gcggctcttg ccgatgcccg cctcgccctc
      121 gatgagcgcc agttggccgt gcccttgaac ggagacgcgc caggcgcgca gcaattgccc
      181 ccactctgcc acgcggccga ccaggggggt tatcccgggt agcacgaccg gcggcgtgac
      241 cgccgtatcg gccgtcagga gctgctgata gagcgcgcgc gtgtcgggat cgggctcgac
      301 gccgaactcc tcctcgaggc gtgccgcgta ggcgcggtag gcccgcaggg ccgacgcgcg
      361 ctggccgttg agggcgtgca ggcgcatcag cgcgcggcag gcggtgtcgt gcagggggtc
      421 gatcgtcagc acccgctcgg cgtagcgggt ggccgcggcg tagtcgtgcg cttcttccag
      481 cgcgatgacg agctgcccga gcgcccagat ggagaggcca cgcagtcgct cgcgctctcc
      541 ggtaatccag tcgtcgtagc aaccgggcag cagatcgccc cggtaggtcg cggccgcctg
      601 cgccagcgag gagcgaaacg acgagccgtc ggtggacttt tccgcccgcc gcccggccgc
      661 gatcgcggcc tcgaactcgt ccacgtcgag cgtatacggg gcattgggcc gccactggag
      721 cgtgctcgtg ccgatcgcca gatagcggtc cggctccggt agggcgtggc gcaggttgtg
      781 gagctcgcgg cgaaggttgg tacgcgcctg tttctcggtt gagtccggcc agaagaggta
      841 ggccagctgc tggcgagcct gcggagcgtc gcggtgcagc aggagatagg ccaggagcga
      901 ctggaggcgc ggtgtatcga cacccgtgat cggcgtcccg ccgtcgacga gcgagaagtc
      961 tcccaagagc gtgatgtgga ggctgggagg catgaggata tccgatcgca acggccgcgg
     1021 cgagggtggc cgtataaacc gccggtcggt ggagagcgag tcccgcgtat cagttacccc
     1081 tatcctgcgg atcttctcgg cttgcgtcaa ggggtgtacc gaaagtgatg atctgcgcgg
     1141 cgccgagcga gtggactgcc ccgtccggcg ccacgtaccg cccgggcgtg attccgcccg
     1201 ggctggcgtc gtgccgcccg gcggcgcttg acgcgcggcg cgaccgagtg gcagagtttg
     1261 tccggctccc cgccgctgaa cgttgacctc gaaccagtgt acggtcgtct tggccggctc
     1321 ggcgtggcga gtggaggctc caacagctca ggaaggtcct gtcggtgtca cgctatcgct
     1381 cgccgtactc ccgtcagccc cgccgccgcc gcgtgctgcc gacgctcgtc gtcagcgtgc
     1441 tcggcctgct ggtgatcgtg ctgggcctct tcgtggtggc cagccacctg ggcgcgaccg
     1501 agggcgtgac gacgcggtcc ggcatcgatc gcaccccgcc ccccacggcc gccgcgaccg
     1561 tacccggcgc cagccccgcg gccaacctcg ccgccagccc ggccgcctcg cccaccgtca
     1621 accccgccac cctcaaatcg ccgctcgacg tggccaaggc ctacgtccag ctctggaacg
     1681 gcggcaactt cgacggtatg taccagttga tctccaccgt ggcgcagtcg accatcagtc
     1741 aaaaggactt cagcgggcgc tatcagggga tcgatcagga ggtcgggcag agctcgatca
     1801 aggtcacgat caacaacaac acgccggacg acgccaccca cgtcccgatc cacgtcgcgc
     1861 gcgagtcggg caaggtgggt cacctggagg aggacaacct cattccactg gtgaaggacg
     1921 gcgccaactg gcgcatcgac tggacgccgt cgctgatctt cgccgacctg ggcgacggct
     1981 tcgtgcgctg gaagccggac gtgccccagc gcggccgcat cctcgaccgc aagggccgcc
     2041 cgctggccca cctcggcacg atcagcaagg tgggcatcgt gccgggcgag atcaaggacg
     2101 aggcggccat gctcgatcaa ctgagccagc tcctcaacat gcccaaggac acgatcaaga
     2161 gcctctacgc caatggccag ccggactggt tcatgccgat ccacaactac cccgacccga
     2221 tggacccgac gctggagcag aagctgaagg cgatccccgg catcgagatc cagaagtggc
     2281 cggatcgggt ctaccccgac ggcgccgcct cggcgcaggt cgtcggctat cttagccagg
     2341 tgacggccga cgagctgccg gagctctcca agcagggcta cagcgccggt gacatggttg
     2401 gccgggcggg catcgaggcc tggggcgaga aatacctggc cggcaagcgc ggtggtcgcc
     2461 tggtgatcgt cgggtcggac ggctccgaac gcaagacgct gggcgaagtc aagagcgagc
     2521 cctccgacga catcgtactg acgatcgacc tcgacctgca gaacgcggcc ttccagttgc
     2581 tggccgacaa gcccggcagc atcgtggtgc tcgaccccaa cagcggcgcg gtgctggcga
     2641 tggccagcaa cccgagcttc gaccccaatc agttcatcct cggtctctcc gaccagcagt
     2701 ggaccgacct gaacaacccc cagacgcacc cgctgcagaa ccgcgccact caatcggcgt
     2761 acccaatcgg ctcgacgttc aaggtcgtca ccatggccgc agctatgcag tcgctggggc
     2821 tgacgccgag cagcacgatc ccctgccccg gcagcttcac catccccggc ggcagcaccg
     2881 tctggcacga ctggagcctg accgggcagg ggacgctgac gctgcacaac gcgctggtgc
     2941 agtcgtgcga cacggtcttc tacaagctgg gcgccgatct cgacaccaag gaccagaacc
     3001 tcttgccgaa catggcgcgc gcctttggtt tcggtaagcc gaccggcatc gagctgcccg
     3061 aagtgccggg ggtcgtgccg gacgccgagt ggaaactcaa ggcgaccggc gactactggg
     3121 cgcgcggcga cgccgtcaat ctggcgatcg gccagggcta cttcctgggc acgccgctgc
     3181 aggtggccga tgcctacgcc gccatcgcca acggcggcac gctctggcag ccctacgtgg
     3241 tgcaggaggt ccacaagctg gacggcacca aggtctacag cgcgcagccg aaggagcggg
     3301 gcaagctgcc gatcacggcg gatcaggtgg gcgcgatccg ctcggcgctc aaggacgtgg
     3361 tcagcgcgcc caacggcacc gccgtggccg ccttcaaggg cgagcagcat cccgtggcgg
     3421 ggaagaccgg caccgccgaa accaacaagc cggagccgca ctcctggttc gtgagcttca
     3481 ccccggtcga cggcgccaag ttcgcctcgg tcacggcgat cgagaacgcc ggcgagggcg
     3541 gtgacatcgc cgcgccgatc gagcgccagc tcatcgacaa gtactaccag ctctaccctt
     3601 gagcctgacc ggagacagca gcggcccgca ccattggtgc gggccgccat gtcgcgtgtg
     3661 ttgccagcgt acggcgacgg ctactggacg gtaacgtggc cgaccatacc acccgcttcg
     3721 tgcgggatgc agtagtaggt gtagtctccc gccacgtcga aggtgtgctt gaaggtcttg
     3781 cccgggccga cgttgccgga gtcccaggct tgcgcgcccg aggggagctt ggcgtcggcg
     3841 gggttttgtg ccttgtcatt gtcggtcgcg gtgtggtcca actggcccgt gtttttccag
     3901 gtgatgctgt cacccttctt gatggtgatc gacgccggct cgaacttgag cgcgtcggtc
     3961 atgttcacgg tgtggtcgct gccaccgccc gagctgcccc cattgctgct gccgccgcca
     4021 ccgcaggccg ccagcagccc gcccaccgcg aacgcggccg gaaccgccgc cgcgtagcgc
     4081 agcacgctgc gccgagagat cgtcttgggc atcgaatcac gatcgctgag cgtcatgaag
     4141 cttccttcct gcctgaacag gcatgtgccg aatccgtggc aacaaactcg ctcctcctcg
     4201 cacaagggat gccggccctt gtgcccactc caatcatccc ccaatctcac agtgtgccgc
     4261 atcacgccga ctctgtgcgt caggagaaac ccgcgctcag gggccgtgtc tggccagcgc
     4321 cccccaggaa atcccgcacc gcggtctcgc tctcggggtt ggcgagcacc gcgaccagat
     4381 cgccggcctc cagcgtcgag ctgccgcgcg gcagcaccgt cgcgccgctc cggtggatcg
     4441 agaccaccag cgcgttgccg gggaagtgca gctcgcgcag cgccttgccg gccagcggcg
     4501 agccgggcgc gatgcgcgac tccaacagca cggtgtcggc cgtgatcccg tgcaggcgcc
     4561 gcacggtcca gcgcaacgcg gcgcggtagg tcgccacgat atcggcggcg gtgatgactc
     4621 ccaccacccg atcgcgcgcc tcgaccaccg gcgcccagtt cacctggttg gtcgtcaaca
     4681 gctccagcgc cacgtccagg ctgctatcgc tgtcgatgac gaccggcggc gctgccacga
     4741 gcgtcgcgac cgtcgtgtcg gcgcgctggt cctccgcgac cgaggcgaga tcgtcgattc
     4801 ggatgactcc gacgagctgg tgatgctcat cgacgaccgc agcgcccggc agccggtgcg
     4861 cgcgcaacac gcgttcggcc tcggccagcg gctgccgggg ggtcagcagc accaccgcct
     4921 taccgatcgc gtcgcgtacc gccaaggtgt tgagcagcgg gaagctgaac tggtagcggt
     4981 gggccggcga gtcggcgcgc gtcgggagct ggctggtata gatcgtgtgc tcgccggtga
     5041 ccagtgtggc cagtccgacc gcgatcatgg ctggcgccag cagcgagagg ttgccggtca
     5101 tctcgcccac catcagcatc accgccagcg gggcgtgggc gatgccgccg aagagcgcgg
     5161 ccatggcgac gatggtgaag ggggcggcgc tctcgggcat gccgggcagc atggcgtggc
     5221 tgacttgcca gaaggcccaa ccgagcgagc cgccgatcac catgccgggg ccgaagatgc
     5281 cgccggagcc gcccgaaccg acgctcagtg aggtcgccag gatcttggca aaggggagga
     5341 gcagcagcgc ccagagcgag atggtcagcg gcgccccaag cccctcctgc agccagccgt
     5401 agccggtgcc gagcacctgc ggcagaacca gcccgaggag cccgaccagc gcgcccccaa
     5461 tgaccggctt gagccaggcg ggcagcggca gccggcggaa gatgccggcg acactgtaga
     5521 acgttttggc gtagagaatg ccgaccacac cgcagaggag cccgagcagc gcgtagtaaa
     5581 ggagttggac gggactggtg aagccgagct ggggctgcgc gccgaagatc ggctcccagc
     5641 cggcccaggc gccgaagatg gtatagccga cgatcgaggc gatcagaccg gggaagagcg
     5701 cttcgatctc catgtcgtgg gtgtagagga tctcggccgc cagcacggcg ccgccgagcg
     5761 gggcgcggaa gatggcgccg atgccggcgc cgatgccggt ggccagcagg atgcgccggt
     5821 cgttggggtc gagcccgagc cactggccga gcagcgagcc gacaccggcc gagatctggg
     5881 cggtggggcc ttcgcgcccg gccgagccgc cggtgccgat ggtgatggcc gaggccagcg
     5941 tcttgaccgg gatgacgcgc gcccgcacgc gcccgccctt gtggtggaag gcgtcgatgg
     6001 cggcgtcggt gccgtggccc tcggcctccg gcgccagggt gaagaccagc agcccgctga
     6061 gcagcccgcc gagcgcgacg accagcggca gcgcccaggg acgcgccagg ctggaggcgc
     6121 cgtggttgcc ctcgccgagc ggggcgggcg gcgcgtagcc cgccagccgg ccgaggaaga
     6181 ggtgcgtacc ggtctggagc atccagaaga agacgatggc gcccagcccg
//
LOCUS       DASUBI010000616         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_494450, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000616 DASUBI010000000
VERSION     DASUBI010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494450"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..988)
                     /locus_tag="VFU72_11875"
     CDS             complement(95..988)
                     /locus_tag="VFU72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424233.1"
                     /translation="MEMAIVTEDLTKTYADVLALDRLNLRVPQGEIFGFLGPNGAGKT
                     TTIRLLLDLIRPSAGRASVLGFDCQQQSLEARSRIGYLPGDLRLYPGLHGHDMLDLVA
                     HLRATPVDRRYVQRLVDRLELDLRPRIATYSKGTRQKLGVVLALLARPPVLLLDEPTS
                     GLDPLMQHAVWDILREEAERGTTVFFSTHVLSEAALICQRVGILGAGRLIAVEPIAQL
                     QRRALRRLVVTFAGTVPELGALPGVRLVARAEHQLTLETPDDIDPLVKRLAGFHVCDL
                     RTEQPDLDDILLGYYPESRTP"
     gene            1262..1990
                     /locus_tag="VFU72_11880"
     CDS             1262..1990
                     /locus_tag="VFU72_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424234.1"
                     /translation="MKITTHGEYLTQLTNWPLLFPVNVYLVREDDGLTLVDAGLGGMA
                     PGILRAAQAFGAPIRRIVLTHAHADHVGSLDTLHAALPEAEVLMSARSARFLAGDRTL
                     DEAEQVGKLRGGWKVAATRPTWLIQPGERVGSLEVVAAPGHSPDHSALFDTRDRTLIA
                     GDAFQTRAGIAVSGTIRPLFPFPALATWHKPTALDSARRLRALEPARLAVGHGPVLGE
                     PLAAMDRAIVTAERKLAGAVQHAG"
     gene            1980..2558
                     /locus_tag="VFU72_11885"
     CDS             1980..2558
                     /locus_tag="VFU72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEU5424235.1"
                     /translation="MPARARAGLNREIVVRAAAELLDAVAGREVTLAEVASRLGVRTP
                     SLYNHIGGQEELRRELALLGLRELGARLGRAAIGKAGEEALLAIGDAYRAFAKERPGL
                     YLATLRAPAPDDAAHLAASQEILDVLHLVLAPYGLGPEEEVHAIRGLRGLAHGFVALE
                     LAGAFGLPVDTDASYQRLLARYIQGLRQAPAA"
     gene            2726..3016
                     /locus_tag="VFU72_11890"
     CDS             2726..3016
                     /locus_tag="VFU72_11890"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5424236.1"
                     /translation="MHGTPLIILEIFAGIAFLTVLGAAIGVVMGRMFGTEPPGGKPES
                     ILRQRYARGELTREQYQQMRWDLGLGSEPSPGSVDGSRAAKPDVAGSQHAAS"
BASE COUNT          483 a         1090 c         1124 g          444 t
ORIGIN      
        1 gtgaccatca cggcgatcgc ggcgttggtg agcccaaccc cgatcaaggc tccgcgatgg
       61 tccgcgacac cacggctgaa gaggctgtga cgcatcatgg cgtcctcgat tccggatagt
      121 agccgagcag gatatcgtcc aggtccggct gctccgtgcg taggtcacag acgtggaagc
      181 cggccaggcg cttgaccagc gggtcgatgt catccggtgt ctccagcgtc agctggtgct
      241 cggcgcgagc gacgagtcgt acgcccggca gcgcccccag ctcggggacc gtgccggcga
      301 acgtcaccac cagccgccgc agggcgcgcc gctgcagctg agcgatcggc tcgaccgcga
      361 tgagccgtcc ggcgcccagg atgccgacgc gctgacagat cagcgccgcc tcgctgagca
      421 cgtgggtgga gaagaagacg gtcgtgcctc gctcggcctc ttcccgcagg atgtcccaca
      481 cggcgtgctg catcagagga tcgagcccgg aggtcggttc gtcgagaagg agaaccggcg
      541 gacgcgccag cagcgccaac acgaccccga gtttctgtcg cgttcccttg gagtaggtgg
      601 cgatgcgcgg gcgaagatcg agctccaggc ggtcgacgag ccgctggacg tagcgccgat
      661 cgaccggcgt ggcgcgcagg tgcgccacca ggtccagcat gtcgtgaccg tgcagccccg
      721 ggtagaggcg cagatcaccc ggcaggtagc cgatgcgaga gcgggcctcc agcgactgct
      781 gctggcagtc gaacccgagg acgctggccc gcccggcgga ggggcggata aggtcgagca
      841 gcaggcgaat cgtggtggtc ttgccggcgc cgttcggccc gagaaagccg aagatctcac
      901 cctgcggcac gcgcaggttc agccgatcga gcgccaggac atcggcgtac gtcttggtca
      961 aatcctcggt cacgatggcc atttccatcg ccgactcccc gtcgtagcag gaacccatga
     1021 ccgtcccgga gcagcgcccc gcgccgccgc gatcatcgta cggcggccgc cgtctcagcc
     1081 ggatcgcagg cagcgccgcg aaccttacaa acgcgtgaca ccctgaagat gagcggccgc
     1141 gcgccggtgg aggtcctgca gccgcggaca gagcaacggc ttgatagact acggttcata
     1201 gtttataatg gaagggtgat agtaacgcgg ccgggcgacc ggccagaaag ggacctgatc
     1261 ggtgaagatc acgacccacg gcgaatactt gacgcagttg acgaactggc cgctgctctt
     1321 cccggtcaac gtctatctgg tgcgtgaaga tgacggcctg accctggtcg acgccggcct
     1381 gggcggcatg gcgccgggca ttctgcgtgc cgcccaggcg ttcggcgccc cgatccggcg
     1441 catcgtgctg acgcacgcgc atgccgacca cgtcggctcg ctcgacacac tgcacgccgc
     1501 gctgcccgag gccgaggtgc tgatgagcgc gcgcagcgcc cgcttcctgg ccggcgaccg
     1561 cacgctcgac gaggccgagc aggtgggcaa gctgcgcggc ggctggaagg tggccgccac
     1621 ccggccgacg tggctgatcc agcccggcga gcgcgtcggc tcattggagg tcgtggcggc
     1681 gcccggccac tcacccgacc acagcgccct cttcgacacg cgcgaccgca ccctgatcgc
     1741 cggcgatgcc ttccagacgc gcgccggcat cgccgtctcg ggcacgatcc ggccgctctt
     1801 ccccttcccg gcgctggcga cctggcacaa gccgacggcg ctggactcgg cccgccggct
     1861 gcgggcgctg gagccggctc ggctggcggt ggggcacggt ccggtgttgg gcgagccgct
     1921 ggcggccatg gaccgggcca tcgtcacggc cgagcgcaag ctggcgggcg cggtccagca
     1981 tgccggctag ggcgagggcg ggtctgaacc gggagatcgt ggtgcgcgcg gcggccgagc
     2041 tgctcgacgc cgtcgccggc cgggaggtaa cgctggccga ggtcgcgtcc cggctcgggg
     2101 tgcgcacgcc ctcgctctac aaccacatcg gcgggcagga ggagctgcgg cgcgagctgg
     2161 cgctcctcgg cctgcgcgag ctgggcgcgc gcctgggccg cgccgccatc ggtaaggccg
     2221 gcgaggaggc gctgctggcc atcggcgatg cctaccgcgc cttcgccaaa gagcggcccg
     2281 gtctctacct cgccacgctg cgcgcccccg cgccggacga cgcggcgcat ctggccgcca
     2341 gccaggagat cctcgacgtg ctgcatctgg tgctggcgcc ctacggcctc ggccccgagg
     2401 aggaggtgca cgccattcgc ggcctgcgcg gcctggcgca cggcttcgtc gcgctggagc
     2461 tggccggcgc cttcggcctg ccggtcgaca ccgatgccag ctaccagcgg ctgctggcgc
     2521 gctacatcca ggggctgcgc caggctccgg cggcctgaat ccgcctggcg cctccgcgcg
     2581 atgacacgat tctgatacac ccccgctcca aacctgaccc aactgacacg cgctgcccgc
     2641 taccgttggg ctgcaacgcg gcgcgcggcc gggcgaggcg accgcccccg ttcgcgcgcg
     2701 ccgtgacgcg gaaaggagac cggtcatgca tgggacaccc ttgatcatcc tcgagatctt
     2761 cgccggcatc gccttcctga cggttttggg cgcggccatc ggggtcgtga tgggccggat
     2821 gttcggcacc gagccgccgg gcggcaagcc ggagtcgatc ctgcggcagc gctacgcccg
     2881 aggcgagctg acacgcgagc agtatcagca gatgcgctgg gatctggggc ttgggagcga
     2941 gccgtcgccg ggttcggtgg atggttcacg ggccgcgaag ccggatgtcg ccggctcaca
     3001 gcacgccgcg tcgtaagcgg cttcacggcc ggaatggagg cgccggagcg tgacacacat
     3061 tcagggcgag atcgtgatca atcggccgct ggatgaggtc ttcgacttcg tggtcgacga
     3121 gcgcaacgag ccgcgctaca a
//
LOCUS       DASUBI010000617         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_251532, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000617 DASUBI010000000
VERSION     DASUBI010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251532"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..2412)
                     /locus_tag="VFU72_11895"
     CDS             complement(346..2412)
                     /locus_tag="VFU72_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5424237.1"
                     /translation="MRQPRLLLLLALLLVCGSACRGPFATTAGGATPASATAGTLAAT
                     PSPVPSPTAAALATPPPAPSRATGSPSPAAASPVASPAAAIDGQLAIVRAGYDLLLDD
                     FYRQLDPRSLLTVAWASLGRDASACHDPAPPALPPLAPDRVLAFASVQDRYRAYQAGL
                     AQPLPATTVAQHMLDAMARSLNDDHTHFLPPATYQSFLNSVTGQEQVGFGMRLSYLYP
                     LVVTRTAPGGPADRAGLKAGDAIVGLNGTNTRFLSPGVFNAALAAAREPTTLTVDRGG
                     EEFDLRLRRGPYFYPPWESRMLPSGVAYLRLDTFLSSGRVLPNGQEILADLDQRLDQF
                     DAQHASGLILDLRDNSGGSVLTSSEILGRFLPEEDLTIHNHDEHGGDSANLVSGQMHK
                     TQLPMVVLVNGGSASSSEATAAALQENGRALLVGTRTAGALATSEILPLPDGSGMQIA
                     VKSVVSAKGMVIDKVGLAIDVAVEDTRTADDYRLGHDPQLDAAARALADAPPPPAYHT
                     TLTALNAQQLDQRLGVALPDPAQLPVNDRLTGDTVTYRHDLTHVNEWIGGDRDPLAEQ
                     QRVRARGYEGSVVASYGIGPGQEPSLTVHLDIYASDEGAKAALDAMVPSSTRDVIASP
                     VQLGDQTVATLGKGLGLGSRSITWRHGNVVIGVSYADVPGYDRPDTLVAAARVADTDY
                     LSRAGH"
     gene            complement(2629..3075)
                     /locus_tag="VFU72_11900"
     CDS             complement(2629..3075)
                     /locus_tag="VFU72_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424238.1"
                     /translation="MLKRLFTRTQPDNPVQPQRLVPTIPGAYLFTLRSVPRHYAGIKA
                     AIESTGEARVYFGEPLAYLKGQGVALFRVEATGFGWLDNLYAWWRQAESAEATGFDIR
                     LFVENTRYVARLRDLTPAEVEVLITRNATRNEQPGEAPTLSSNKAP"
     gene            complement(3460..>3941)
                     /locus_tag="VFU72_11905"
     CDS             complement(3460..>3941)
                     /locus_tag="VFU72_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424239.1"
                     /translation="YWDKWLIKDGLTLSPNYTVTAKLNFQTGVADRAGITIGWKDVTL
                     DRIDIQPNVAGDDIEFRASAGIGSSYTVSGSATTGGGLPINANTDYWLRVSTSIAANG
                     DGVLVVSWSTDGVHFSEVERAVGPSLGGQAMVGQVGFGTAGPNMPNVWFDDFTVTEP"
BASE COUNT          597 a         1256 c         1429 g          659 t
ORIGIN      
        1 aagcgctacg aggagcaccg gcgcctgatc cgcatcgtgg ccagcgagcg cctgcgcgac
       61 gaggtcgccc gccgctggga acacctgctg ctgccgctgc tcggcggcgc ggcgctcacg
      121 cctggctcca gcccttcgac aaggtcttga ggcgggtgag gacggatcct tcgactgcgc
      181 tcaggatgac agggtaagag aggggctgac gaggaaggct tgcctcgtcc ggtaggccgc
      241 atcccttacc ctgtcatcct gagcttgtcg aaggatccgt gccgcacgag cgtcccgccc
      301 gcatcttcag ctacgatccc gcggaggcgt tctgagcggt tccccttaat ggcccgcgcg
      361 gctcaggtag tcggtgtcgg cgacgcgtgc cgcggcgacg agggtgtcgg gacggtcgta
      421 gccgggcacg tcggcgtagc tgacgccgat gacgacgttg ccgtggcgcc acgtgattga
      481 gcggctgccg agccccagcc ccttgccgag tgtcgccacc gtctgatcgc cgagctgaac
      541 cggactggcg atgacgtctc gggtcgacga cggcaccatg gcgtcgagcg cggctttggc
      601 cccctcgtcg ctggcgtaga tatcgaggtg gaccgtcagg ctgggctcct ggcccggacc
      661 gatgccgtag gacgcgacca cgctcccctc gtagccgcgt gcccgcaccc gctgctgctc
      721 ggccagcgga tcccggtcgc cgccgatcca ctcgttgacg tgcgtgagat cgtggcgata
      781 ggtgacggtg tcgccggtca gccggtcgtt gaccggcagt tgggcgggat cggggagcgc
      841 cacaccgagc cgctggtcga gctgctgggc gttgagcgcg gtcagcgtcg tgtggtaggc
      901 cggcggcggg ggcgcgtcgg ccagggcccg ggcggccgcg tcgagctggg gatcgtgccc
      961 caagcggtag tcgtccgccg tgcgggtgtc ctccaccgcg acgtcgatgg ccagcccgac
     1021 cttgtcgatg accatgccct tggccgacac gaccgacttg acggcgatct gcatgccgga
     1081 gccgtcgggg agggggagaa tctccgaggt ggccagggcg ccggcggtgc gcgtgccgac
     1141 cagcaacgcg cgcccgttct cctgcaaggc cgcggcggta gcctcggacg aggaggccga
     1201 gccgccgttg accagcacga ccatcggcag ctgcgtcttg tgcatctggc cgctcaccag
     1261 gttggcgctg tcgccgccgt gctcgtcgtg gttgtggatc gtcagatcct cctccggcag
     1321 gaagcgcccc aggatctcgc tggaggtgag gacgctgccg ccggagttgt cgcgcaggtc
     1381 gaggatcagc ccggacgcgt gctgggcgtc gaactggtcg agccgctggt cgaggtcggc
     1441 caggatctcc tgcccgttgg ggaggacgcg gcccgagctg aggaacgtgt cgaggcgcag
     1501 ataggccacg ccgctgggga gcatgcggga ttcccagggc gggtagaaat aggggccgcg
     1561 gcgcagtctc aggtcgaact cctcgccgcc gcgatcgacg gtgagggtgg tcggctcgcg
     1621 cgccgcggcc agcgcggcgt tgaagacccc cggcgacagg aaccgagtgt tcgtgccgtt
     1681 gagaccgacg atggcgtcgc cagctttgag ccccgcccgg tcggcagggc cgccgggcgc
     1741 ggtccgcgtc acgaccagcg gatacaaata tgagaggcgc atgccgaagc cgacctgctc
     1801 ttggccggtg accgagttga ggaagctctg gtaggtcgct gggggcagga agtgcgtgtg
     1861 gtcgtcgttc aggctgcgcg ccatcgcgtc gagcatgtgc tgcgcgaccg tcgtggccgg
     1921 cagcggctgg gcgagcccgg cctggtaggc gcggtagcgg tcctgcacgc tcgcgaaggc
     1981 gagcacgcga tccggagcga ggggcggcag cgccggcggc gccggatcgt ggcaggcgga
     2041 cgcgtcgcgg cccaggctgg cccaggcgac ggtgaggagg ctgcgcgggt cgagctggcg
     2101 atagaagtcg tcgagcagca gatcgtagcc cgcgcgcacg atggcgagct gcccgtcgat
     2161 ggccgccgcc gggctggcga ccgggctggc cgccgcgggg gatggtgagc ccgtcgcacg
     2221 gcttggagcg ggcggcgggg ttgccaacgc cgcggcggtc ggcgagggga ccggcgaggg
     2281 cgtggcggca agcgttcccg ctgtcgcgct cgcgggcgtc gcaccgcctg ccgtggtggc
     2341 gaacgggccg cggcaggcgc tgccgcagac gagcagcagc gcgaggagaa gcagcagtcg
     2401 aggctggcgc acgttccctc tcccagtctg ctcatgagcc ggaagcccgg cgcgtcagcc
     2461 tggaacccgc ggcgatgccg catcacatcg cgggtgcact ggatgagaag taagcggaat
     2521 gcggtcgatg tgctcagaat acgagcgtgc gagctgagcg tcaagccgcg cgcagggaag
     2581 caggaccgat gggcgtagga cctgcgcctg tctggagagc ggggctggct acggcgcctt
     2641 gttggaggag agcgtcggcg cctcccccgg ctgctcgttg cgcgtcgcgt tgcgcgtgat
     2701 cagcacctcc acctcggccg gcgtgagatc gcggagccgg gcgacgtagc gcgtgttctc
     2761 gacgaagagg cggatatcga agccggtggc ctcggcgctc tcggcctgtc gccaccaggc
     2821 gtagaggttg tcgagccagc cgaagccggt cgcctccacc cggaagaggg ccacgccctg
     2881 gcccttgagg taggccagcg gctcgccgaa gtagacgcgc gcctcgccgg tcgactcgat
     2941 ggcggccttg atgccggcgt agtggcgcgg caccgagcgc agggtaaaga ggtaggcgcc
     3001 gggaatcgtc ggcaccagcc gctgcggctg caccgggttg tccggttgtg tccgtgtaaa
     3061 gagcctcttc agcatgccac ccccaccatt ccgcgccctg tctcgctcgt tgtcacccgc
     3121 gctcagtgcc gcaaggtcgt tgaggctgcg gctgtgggtg attgtacggc agggcggcca
     3181 ggcagtcgcc acaggctgtg ggattctcaa ccccaacctc tcgtaactga ctccgcctat
     3241 gccgtttcga gttgcatttg cgagtcatat tcgagggtaa cagcacgccg cccaaacctt
     3301 gggtggcgtt agaattcgac cgacagttat cgccgtggac cagacgtata aaagtcttgt
     3361 ctggttactg ccagccaagt atcacggctg aacgttgctg cttgcattac cgcacaccgc
     3421 acaccgcaca gcgacagact tgtgtcccag tgcttgccct catggctcag tgaccgtaaa
     3481 atcgtcgaac cacacattcg gcatgttggg tccggcggtc ccgaatccta cttgccctac
     3541 cattgcctgc ccgccgagtg atgggccgac cgcgcgctcc acttcagaga aatgcactcc
     3601 atcggtcgac cacgagacga cgagtacacc gtctccgttg gctgcgatcg atgtcgagac
     3661 tcgcagccag tagtctgtgt tcgcgttgat tgggagacct ccgccagttg tcgcggatcc
     3721 gctgacggtg taagacgatc cgatgccagc gctggctcga aactctatat catcgcccgc
     3781 tacgttgggt tggatgtcga tgcgatccag cgtcacgtcc ttccacccaa ttgtgattcc
     3841 ggcacggtcg gcgacgccgg tttggaagtt gagtttggct gtcacagtgt agttagggct
     3901 gagggtcaag ccgtccttga tcagccactt gtcccaatat t
//
LOCUS       DASUBI010000618         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_17756, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000618 DASUBI010000000
VERSION     DASUBI010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17756"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFU72_11910"
     CDS             complement(<1..770)
                     /locus_tag="VFU72_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424240.1"
                     /translation="MAVATRGRSKTQAHPDAAATADGAQAARGYTGADIRVLEGIEAI
                     RTRPGMYIGSTSSTGLHHLVWEALDNATDEAIAGYGQNIWVEIDGDGWASVRDEARGM
                     PFDPKKINGKTLPTATVILTVPHSGGKFAEGAYKTAGGLHGVGATIINALSEELELTI
                     WRDGQEFHQHFQRGVPGKHTLSKHPGDQHGTLIRWRFDRDIFSEHSLGYQREVVESRM
                     RAAAYLNRGIAYHLRIWDDDSGEMVEQTFFSERGLVDYV"
     gene            complement(1142..>2335)
                     /locus_tag="VFU72_11915"
     CDS             complement(1142..>2335)
                     /locus_tag="VFU72_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5424241.1"
                     /translation="LINRGDLHLESVRFLVLDEADEMLDRGFAPDVERILAHTPRERQ
                     TALIFGGRASGPQVAALRRGAQVVIGTPGRVLDLLDQGALRLDRLRFLVLDEADQMLD
                     EGFAPSVERILARSPRARQTALFSATVPEWVSQTASRHLKHPATVAVDPNPEDAPAIA
                     HTAYQLAGDDKLTALHALLDRRGEGSIIVFGRTKHGVKKLARRLERDGYPVGELQGNL
                     SQNARDRVMERFRSGEVQILVATNVAARGLDITSIALVINLELPESADLLTHRVGRTG
                     RMGREGRAITLLGPEDAVKWRKLARDSRQRIPVLPWPGATAALDAEPAAPPSQTKAHV
                     PAATRRRETPEPSRERRPRQHAPAERTTIVCSGCGQPAEVPFKPDPTRPVYCDDCHRA
                     RRARR"
BASE COUNT          308 a          802 c          846 g          379 t
ORIGIN      
        1 acataatcga ccaggccgcg ctcggagaag aaggtctgct cgaccatctc gcccgagtcg
       61 tcgtcccaga tgcgcaggtg gtaggcgatg ccgcggttga ggtaggcggc ggcgcgcatg
      121 cggctctcga cgacctcgcg ctggtagccg aggctgtgct cgctgaagat gtcgcggtcg
      181 aagcgccagc ggatcagggt gccgtgctgg tcgccggggt gcttgctgag cgtgtgcttg
      241 ccgggcacgc cgcgctggaa gtgctggtgg aactcctggc cgtcgcgcca gatagtcagc
      301 tccagctctt ccgagagggc gttgatgatg gtggcgccga cgccgtgcag gccgccggca
      361 gtcttgtagg cgccctccgc gaacttgccg ccggagtggg gcaccgtcag gatcacggtg
      421 gcggtcggta gggtcttgcc gttgatcttc ttgggatcga agggcatgcc gcgcgcctcg
      481 tcgcgcacgc tggcccagcc gtcgccgtcg atctcgaccc agatgttctg gccgtagccg
      541 gcgatcgcct cgtcggtggc gttgtcgagc gcctcccaga cgaggtggtg caggccggtg
      601 gagctggtcg agccaatgta catgccggga cgggtgcgga tcgcctcgat cccctccagc
      661 acccggatgt cggcgccggt gtagccacgc gctgcctgcg cgccgtcggc agtcgccgcc
      721 gcgtccggat gcgcctgcgt cttgcttcgt cccctggtcg ctaccgccat gctctcttcc
      781 gccccgttcg tgtcccccgg acaaaaccgg cctctctgac gagcggccct tcctagaact
      841 gatgttcgct ttccgcccca attatagcgg gttccggccc atattgccag cccgtcatgg
      901 cgtacgttcg cgaaagtcgt tgagagtgtg ctggccatcg aggacgctgg ccgccgggtg
      961 gggcacggat ccctccaccg cggtcgggat gaccggggaa aggatgaggc ggacgacgac
     1021 gatgatcgcc gtcgtccgcc tcatcccctg acctgtcatc ctgagcttgt cgaaggatcc
     1081 gtcctcacgc ggcacgcgac gatgccgctg gggcagcacc ccgtctcagc gcccgcgcca
     1141 cttaccgccg cgcgcgccgt gcccgatggc agtcgtcgca gtagaccggg cgcgtcgggt
     1201 ccggcttgaa cgggacctcg gccggctggc cgcagccaga gcagacgatt gtcgtgcgct
     1261 ccgccggggc gtgctgacgc gggcgccgct cgcgggacgg ctcgggcgtc tcccggcggc
     1321 gcgtcgcggc cgggacgtgc gccttggttt ggctgggcgg cgcggccggc tcggcatcca
     1381 gcgcggcggt ggcgccgggc caggggagga ccgggatgcg ctggcgcgag tcgcgcgcca
     1441 gcttgcgcca cttgacggcg tcctcggggc cgagcagggt gatcgcgcgc ccctcgcggc
     1501 ccatgcgacc ggtgcggccc acgcggtggg ttagcaggtc ggcggactcc ggcagctcca
     1561 ggttgatcac cagggcgatg ctggtgatgt cgagcccgcg cgccgcgacg ttcgtcgcca
     1621 ccaggatctg cacctcgccg gagcggaagc gctccatcac ccggtcgcgc gcgttctgcg
     1681 acaggttgcc ctgcagctcg ccgaccgggt agccgtcgcg ctccagccgc cgcgccagct
     1741 tcttgacccc gtgcttggtg cggccgaaga cgatgatcga gccctcgccg cgccggtcga
     1801 gcagcgcgtg cagcgcggtc agcttgtcat cgccggccag ttggtaggcc gtgtgggcga
     1861 tggccggtgc gtcctcgggg ttggggtcga ccgccacggt ggccgggtgc ttcagatgcc
     1921 ggctggcggt ctgactgacc cactcgggca cggtagccga gaagagggcc gtctgccgcg
     1981 cgcgcggcga gcgcgccagg atacgctcga cgctgggcgc gaagccctcg tcgagcatct
     2041 ggtcggcctc gtccagcacc aggaagcgca accggtcgag ccgcagcgcg ccctgatcga
     2101 gcagatcgag cacgcgcccc ggggtgccga tgacgacctg agcgccgcgc cgcagcgccg
     2161 cgacctgcgg cccgctggcg cgaccgccga agatcagcgc ggtctgccgc tcacgcggcg
     2221 tgtgggccag gattcgctcg acatccggcg cgaagccgcg gtcgagcatt tcgtccgcct
     2281 catcgagcac cagaaagcga acggattcga ggtgcaaatc accgcgatta atcag
//
LOCUS       DASUBI010000619         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_808440, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000619 DASUBI010000000
VERSION     DASUBI010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808440"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..601)
                     /locus_tag="VFU72_11920"
     CDS             complement(266..601)
                     /locus_tag="VFU72_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424242.1"
                     /translation="MQRAVTRPALAPPAVPPPVERAVTFALLVSAFRCTVQYVLLPFV
                     VPWVGVATLPPWFTLALTLLAGGMLGRNARALWRLRHAQRWTYLLFGVALVGALLLFI
                     AVDLRALLT"
     gene            complement(712..1971)
                     /locus_tag="VFU72_11925"
     CDS             complement(712..1971)
                     /locus_tag="VFU72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5424243.1"
                     /translation="MADVKDPKLTSPDPPTIVAPPEAPVTPDGHPPSVAPAPDGHAAP
                     APPTGALGRMEDQFALRQLISEYLIPVETNTLWYTLGGVLAIALVLEVLTGVLLSFVY
                     APDAGRAYGITRDLLSRPVWSVILNFHYWNAFVIFALVLLHMLRVFVTGGYRGLGQRS
                     KQGLWLTGVVLAGATFIASVTGESLHWDEVGFAVPWHVSEVFQAVGLASAVHYTFADL
                     KNVTSATAKLGQIYAVHISMAPLLLFGFLVLHYYLIKVKGISFPFWRKASGKVAPFSS
                     HIREWLIYGSIILGLVLLVAIFVPRDPGTAPQLLPDSPLYGATHGPGGLGYKPTFPIS
                     WTHGMNVFVGEHLGIEPDIWGSILGMTVMVLALLAIPFVDRGRRELRGWRQAFNLRER
                     GWAFLAMGLFWLILIIGVVQNFVAGAG"
     gene            2277..2708
                     /locus_tag="VFU72_11930"
     CDS             2277..2708
                     /locus_tag="VFU72_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5424244.1"
                     /translation="MPAQDNKALVRQYFEEVRNQHRREAAGALFAADVAVHVDTPTGP
                     ETVHGIRGILETLAPYLAAFPDLHFTIADQLAEADRVMTRWQAVGTHQGPLMGHAATG
                     RRVTFGGIDVFRVANGRITEGWTSYDRLVILQQIGAVSRPQ"
     gene            3132..3413
                     /locus_tag="VFU72_11935"
     CDS             3132..3413
                     /locus_tag="VFU72_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424245.1"
                     /translation="MNQLRGVGHRWERGAEMTVARLAPLMPIFGRRGAGILFSGAAAC
                     GVYCIVYGRRWSLPCYCGAASTAAGMVSTGLHQRSTGGVRSLVGGDQGV"
     gene            3458..3712
                     /locus_tag="VFU72_11940"
     CDS             3458..3712
                     /locus_tag="VFU72_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424246.1"
                     /translation="MKTRNATSPRQQLADALALPAYAHYRADIELHQQQAAQAHHLDP
                     ALPPARRARLREAERLQLAAGQAWLAVERVLHDFDAGARA"
BASE COUNT          667 a         1287 c         1376 g          549 t
ORIGIN      
        1 gtccgacgag ggcgcgatga gcagccagtc aatgccctgc tgctccatcg ccgcttgcgc
       61 ccgcgcaatc cgctcggcat acgccggctg cttcgtggcc tccgcgctca ttcgccgccc
      121 tcctctggct ccctgccacc accggaccag cgcttctgcc ggccgtgccg cgagtatagc
      181 attgcgagcc aggcgggcgg ccgcccaggc gctcgcacgg tgtggtggcc gccatggccg
      241 cgccgatggt caccgcaccc gcctttcagg tgaggagggc gcgcagatca acggcgatga
      301 agagcagcag cgccccaacg agcgccacgc caaacagcag atacgtccag cgctgggcat
      361 ggcggagtcg ccagagggcg cgtgcgttgc gcccgagcat ccccccggcc aacagggtga
      421 gggccaaggt gaaccagggc ggcagggtcg ccacgcctac ccacggcacc acgaacggca
      481 gcaggacgta ctggacggtg cagcggaacg ccgagacgag cagggcgaag gtgacggcgc
      541 gctcgacggg tggcgggaca gccggcggtg cgagggccgg ccgagtgaca gcacgctgca
      601 tgacaattct ccttggtggc taacgctgcg cgtgcgggct ggtcagcagt ggtggttcgg
      661 gccgggcact ccggcggtgc aggtgccgtg agacgcccgg cccggcgcgg cttagccggc
      721 gccagcaacg aagttctgga cgacaccgat gatcaggatc agccagaaga gccccatcgc
      781 caggaaggcc cagccgcgct cacgcaggtt aaacgcctgc cgccagccgc gtaactcgcg
      841 gcggccgcgg tcgacgaagg ggatggcgag gagcgccagc accatcaccg tcatcccgag
      901 aatcgagccc cagatatccg gctcgatacc caggtgctcg cccacgaaga cattcatgcc
      961 atgggtccac gagatcggga acgtcggctt gtagcccagg ccgccaggcc cgtgcgtcgc
     1021 gccatacagg ggcgagtcgg gcagcaactg cggcgcggtc cctgggtcgc gcggcacgaa
     1081 gatggccacc agcagcacga ggcccagaat gatgctgccg tagatgagcc actcacggat
     1141 gtggctgctg aaaggggcga ccttgccgct cgccttgcgc cagaagggga aggagatgcc
     1201 cttgacttta atcaggtagt aatgcagcac gaggaagcca aacaacagca ggggcgccat
     1261 cgagatgtgc acggcgtaga tttgcccgag tttcgccgtg gcgctcgtga cgttcttcag
     1321 gtcggcgaag gtgtagtgca ccgcgctggc caggccaacg gcctgaaaga cctcggagac
     1381 gtgccagggg acggcgaagc cgacctcgtc ccagtgcagg ctctctccgg tcaccgaggc
     1441 gatgaaggtg gcgccggcca ggacgacccc ggtgagccac aggccctgct tgctgcgctg
     1501 gcccaggcca cggtagccgc cggtgacgaa gacgcgcagc atgtgcagca gcaccagggc
     1561 gaagatgacg aaggcgttcc agtaatggaa gttgagaatc actgaccaga ccgggcggct
     1621 gaggaggtcg cgggtgatgc cataggcccg cccggcgtcg ggggcataga cgaaggagag
     1681 caagacgccg gtcagcacct cgagcaccag cgcgatcgcc agcacgccgc cgagggtgta
     1741 ccagagagtg ttggtttcga ccgggatgag atactcgctg ataagttgcc ggagggcgaa
     1801 ctggtcctcc atccggccga gggcaccggt gggcggcgcc ggcgccgcgt gcccgtcggg
     1861 ggcgggcgcc accgagggag gatggccgtc aggcgtcacg ggggcctctg ggggcgcgac
     1921 gatggtgggg ggatcgggtg acgtgagctt ggggtccttg acatcagcca tggggcacct
     1981 cctacagtgg gctgggggtg gccccgctga ccaccaccca cgactacaac cgaccaccat
     2041 acagaaggcg aaatagagat tgcgcctagt ctgcgcgccg acggtaacgg gcgaggtagc
     2101 gcgcggttaa cggcgtgtaa acggttgtta cccggcgggg gccgccggca atcaccagtc
     2161 ggccgtggct cgccttgacg ccagtcacca gcggcgcgag actggacggt ggcacgcgcc
     2221 ggtcgcgcgg cggccgtgtg acagtcgcgg cggtgctggc gcacaggagg tgtgcaatgc
     2281 ctgcacagga caacaaggcg ctcgtccggc agtatttcga ggaggtgcgc aaccagcatc
     2341 ggcgggaggc ggccggcgcg ctcttcgccg ctgacgtcgc ggtgcacgtt gataccccga
     2401 ctggccccga gacggtccat gggatccgcg ggatcctgga gacgctggcg ccctacctcg
     2461 ccgcgttccc cgacctgcac ttcaccatcg ccgaccagct cgcggaagcc gaccgggtga
     2521 tgacccgctg gcaggcggtg ggcacgcacc agggaccgct gatgggccac gcggcgaccg
     2581 gccggcgggt cacctttggc gggatcgacg tcttccgggt ggccaacggc cgcattaccg
     2641 agggctggac gagttacgac cggctcgtta ttctccagca gattggcgcc gtctcccggc
     2701 cgcaatgacg cgcggcgcag ccggatcggc actctctgcc agggtgccgc acaggttggc
     2761 gcgcggtcgt cccgctgcca ctgcacaacc attgagcagc gatctggcgc cgactagccc
     2821 gcgctagcca ggtggacggg tcacccagac ccgcgtgcgg acttccccgg ctcatggcca
     2881 tgagccgggg aagtccgctc agcatgcttg acctggtgtt aacctggagc aaccttgccc
     2941 gtgaccgggg cggcgtagca tggcgctgta cggcggtgta cgccgtggtg ccccgccgtt
     3001 ccggcacccc tgccgggtgc ccgccgacgc tgccgtcggc acgaggcgac gccgccaact
     3061 tccgccccac tgccaagtgc caccctgttg tgcccctcgt catgccaccg ttgccgactg
     3121 gaggtgccgt gatgaaccag ttgcgcggcg tcggccatcg ttgggagcgt ggcgccgaga
     3181 tgacggtggc aaggctggcc ccgctcatgc ccatctttgg ccggcgcggg gcaggcatcc
     3241 tcttctccgg tgcggcagcc tgcggggtat attgcatcgt ctatggccgg cgatggtcgc
     3301 tgccatgcta ctgcggcgca gcgagcacag cggcgggcat ggtcagcaca ggcctgcacc
     3361 agcgctcgac tggcggcgtc cgcagcctgg tgggcggcga ccaaggcgtt tgaaacggca
     3421 ttactgtccg cccagccatt gactcaggag ggacactatg aaaaccagga atgctacgtc
     3481 cccacgccag caactggcag acgcacttgc gctgccggca tacgcgcact accgggccga
     3541 cattgagctg caccagcagc aggcggcgca ggcgcaccac ctcgacccgg cactgccccc
     3601 agcgcggcgg gcgcgcctgc gagaggctga gcggctgcag ctggccgccg ggcaggcttg
     3661 gcttgctgtg gagcgcgtgc tgcacgattt cgacgccggg gcccgggcct agcaacgcgg
     3721 ccactagccc aaggcgggca ggatgaattg aggagccgca accgtgccgc tacggctgcc
     3781 ggcgcagccg acgccctcat cgggcgcgcg gacgtgctga cggcagcgta gcaacaagcg
     3841 atcggcccgc gcgttctagc aggttttcgc tttaaccat
//
LOCUS       DASUBI010000620         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_181421, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000620 DASUBI010000000
VERSION     DASUBI010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181421"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1221)
                     /locus_tag="VFU72_11945"
     CDS             complement(31..1221)
                     /locus_tag="VFU72_11945"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424247.1"
                     /translation="MARRPGLGLSRDNQRIFVAMACNEGAFGIYTTLWPLYIAALGAT
                     PPEIGLVMGFMGLARLLALLPSGLIADRVPPRRLIVGGRSLTVAGMFLLALAQNWWQL
                     IPAAMILTLGNVAFPSISSTIAESAEGDRERTRAFTMIYTVGPSITLLATPALGGLIA
                     DQVGLRAVFAVAAALTGVAVLAFGTITPRPVPAHDGPPATFGEVLAIRPIALIAALEF
                     VTLFILSLGVTLVPNFLQDVHGINTGVIGLLGSFSAVGSIILGIMISRLRPLQRPLAA
                     VALSSATVALSLALLSAGNHLAIFALAYALRGGFSVAWSLFSAAYGEVAPRRLHARTF
                     ALGELSAGLGFSSAPFLAGWLYDLAPVAPLLLALGLAVPLLAAILLVERALRRPRLAV
                     AETV"
     gene            1403..2344
                     /locus_tag="VFU72_11950"
     CDS             1403..2344
                     /locus_tag="VFU72_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5424248.1"
                     /translation="MLERVSGQRVTWIERFPTGLAHYVYDARTDDGEPYVVRLARPER
                     RADFAGAVYWHERLAPLGVPLPVLLHVDPVGELTGFPALVLERLPGTDLGAVYRALSD
                     AERRRLAGEIVAIQRLVAGLPPGPGYGFARSYADPALFPTWSDLLDAQLARARHWMRT
                     AGVVDVTLIDRVAAALEAGRPYFATVEPRCFLDDMTTKNVIVHRGRLSGVVDVDAVCF
                     GDSLLTPALTRMALLSQGEDPSYVAHWLDLLDLSPAQSAALTLYTALFAIIFLGELGQ
                     AFNRDAAPEIDQDRLRRLLAILDSLLAEAERQRTRFT"
     gene            complement(2355..3263)
                     /locus_tag="VFU72_11955"
     CDS             complement(2355..3263)
                     /locus_tag="VFU72_11955"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5424249.1"
                     /translation="MTHRLQHRLTALLLLAALLAGCTPGGGGTATPAPTPAAPVPAFD
                     HIYLLIFENKEESRVIGNIAAPYLNSLARDYGLAVNYYAVAHPSQPNYLALFSGSTQG
                     VSDDGHYDLAGRNLADQLEEHGRSWRAYAENVPPGCYKGDRADDGADGPGTYARKHEP
                     AISFIDISGDPARCANIGDFSHFDPAAANFELITPNLCHDMHDCTVAAGDRWLRGWLP
                     GHLLDSDAWRGSNSLLVITWDEGVTNAGGGGRVATIVISPRTPPGYRSSLRHDHYSLL
                     RTIEASWGLGCLNQTCGANDLAEFFR"
     gene            3580..>4376
                     /locus_tag="VFU72_11960"
     CDS             3580..>4376
                     /locus_tag="VFU72_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEU5424250.1"
                     /translation="MIDLNSDTASRPSAEMRQFMAEAPVGDEQLGEDPSVNQLQDMTA
                     ALLGKEAALFLPSGTMCNAIGIKVHTQPGDRIVLERYAHPFTSEGAGPALMSGIMTAT
                     VEGVRGVFQPEQVAEIASPANNHVPPTTLVCLENTHNRGGGKVWPLDTLNAVAQTAHQ
                     LGLKTHLDGARLLNAAVASGIPAATWAENMDTVWIDLSKGLGAPVGGVLAGDAETMRR
                     ARRYKHMFGGAMRQAGIIAAGGIYALEHNVERLAEDHANARRLAEGLA"
BASE COUNT          675 a         1504 c         1580 g          617 t
ORIGIN      
        1 cccgtccaaa aatcggcacg atcctctccc tcagaccgtc tcggccacgg ccagccgcgg
       61 ccggcgcagc gctcgctcga ccagcaggat agccgccagg agcggcacgg ccagccccag
      121 cgccagcagg agcggcgcga ccggcgccag atcgtagagc cagccggcca ggaagggcgc
      181 cgaggagaaa ccgaggccgg cgctcagctc gcccagcgcg aaggtgcggg cgtgcaggcg
      241 gcgcggcgcg acctcgccgt aggcggccga gaagagcgac caggctaccg agaatccgcc
      301 gcgcagggcg taggccagcg cgaagatcgc caggtggttg ccggccgaga ggagcgccag
      361 cgacaacgcc accgtcgccg aggagagggc caccgccgcc agcggccgct gcaacggcct
      421 gaggcggctg atcatgatgc cgaggatgat gctgccgacc gcggagaacg agcccagcag
      481 gccgatgacg ccagtgttga tgccgtggac atcctgcagg aagttgggga cgagcgtgac
      541 gcctagcgag aggatgaaga gcgtcacgaa ttcgagcgcc gcgatcagcg cgatcgggcg
      601 gatcgccagc acctcaccga aggtagcggg cggaccgtcg tgggccggca ccggtcgggg
      661 ggtgatggtg ccgaaggcca gcacggcgac accggtcagc gccgcggcca cggcgaagac
      721 ggcgcgcagc ccgacctgat cggcgatcag gccgccgagc gccggggtcg ccagcagcgt
      781 gatggatggg ccgacggtgt agatcatggt gaaggcgcgg gtgcgctcgc ggtcgccctc
      841 ggcgctctcg gcgatggtgc tggagatgga ggggaaggcg acgttcccca gtgtgaggat
      901 catcgcggcc gggatcaact gccaccagtt ctgcgccagc gccagcagga acatgccggc
      961 cacggtcagc gagcggccgc cgacgatcag gcgtcgcggc gggacgcggt cggcgatcag
     1021 cccgctgggc aggagggcca gcaggcgtgc caggcccatg aagcccatca ccagcccgat
     1081 ctcgggcggc gtggcgccga gcgcggcgat gtagagcggc cagagcgtgg tgtagatgcc
     1141 gaaggcgccc tcgttgcagg ccatcgccac gaagatgcgc tggttgtctc ggctgagccc
     1201 cagccccggt ctgcgcgcca cgctctccgc cccccacgcc gcgactctga cggggtacta
     1261 gtatagcgag cgctcggctt ccgcgccctc aatcctcgcc gtacgggggc gcgactcgcg
     1321 ccggctgatc cggtgtataa cccgcgctat gagcgacgaa actgggaacg cccgcgatcc
     1381 cggtgaggtg gatgccaggg ccgtgctgga gcgagtcagc ggccagcggg tgacttggat
     1441 cgagcgcttc ccaaccggcc tggcgcacta cgtctacgac gcccgcaccg acgacggcga
     1501 gccctacgtc gtgcgactcg cgcggccgga gcggcgcgcc gacttcgccg gggcggtcta
     1561 ctggcatgag cggctcgcgc cgctcggtgt gccgctgccg gtgctgctgc acgtcgatcc
     1621 cgtgggcgaa ttgaccggct ttccggcgct ggtgctggag cgcctgccgg gcaccgacct
     1681 cggcgccgtc taccgtgcgc tgtcggacgc cgagcggcgc cggctggcgg gcgagatcgt
     1741 cgccatccaa cggctcgtgg ccggcctgcc acccggcccc ggttacggct tcgcccgctc
     1801 ttacgccgat cccgcgctct tccccacctg gagcgacctg ctcgatgccc agctggcgcg
     1861 tgctcgccac tggatgcgga ccgctggcgt ggtggacgtg acactgatcg accgcgtcgc
     1921 ggctgcgctg gaggcggggc ggccctactt cgcgaccgtg gagccgcgct gcttcctcga
     1981 cgacatgacg accaagaacg tgatcgtgca ccgcggccgg ctcagcggcg tcgtcgatgt
     2041 cgacgccgtc tgcttcggcg actcgctgct gacgcccgcg ttgactcgca tggcgcttct
     2101 ctcccagggc gaggatccaa gctacgtggc ccactggctg gatctgctcg acctcagccc
     2161 agcgcagagc gccgccctga cgctctacac cgcgctcttc gccatcatct ttctcggcga
     2221 gctcgggcag gcgttcaacc gcgacgcggc gcccgagatc gaccaggacc ggctgcggcg
     2281 cctgctcgcg atcctcgaca gcctgctggc cgaggccgag cgccagcgga cccgtttcac
     2341 gtgaaacggc ggtgtcagcg gaagaactcg gccaggtcgt tggcgccgca ggtctggttg
     2401 aggcagccga ggccccagct cgcctcgatg gtgcgcagca gcgagtagtg gtcgtggcgc
     2461 aggctggagc ggtagccggg cggcgtgcgc ggcgagatga cgatggtggc caccctgccc
     2521 ccgccgcccg cgttggtgac gccctcgtcc caggtgatca ccagcaagct gttggagccg
     2581 cgccaggcgt cgctgtcgag caggtggccg gggagccagc cgcgtagcca gcggtcgccg
     2641 gcggccaccg tgcagtcgtg catgtcgtgg cagaggttgg gagtgatcag ctcgaaattg
     2701 gccgcggcgg ggtcgaagtg gctgaagtcg ccgatgttgg cgcagcgcgc cggatcgccg
     2761 ctgatgtcga tgaaggagat ggcgggctcg tgcttgcggg cgtacgtgcc cggcccgtcc
     2821 gcgccgtcgt cggcccggtc gcccttgtag cagccgggcg gcacgttctc ggcgtaggcc
     2881 cgccagctcc gcccatgctc ttcgagctgg tcggccaggt tgcgcccggc cagatcgtag
     2941 tggccgtcgt cgctgacacc ctgggtcgag ccggagaaga gcgccaggta gttgggctgc
     3001 gaggggtgcg ccaccgcata gtagttgacg gccaggccat agtcgcgggc caggctgttg
     3061 aggtagggcg cggcgatgtt gccgatcacg cgcgactcct ccttgttctc gaagatcagc
     3121 aggtagatgt ggtcgaaggc cggcaccggc gcggcgggcg tcggcgccgg ggtggccgtg
     3181 cccccgccgc cgggcgtgca gcccgccagc agcgccgcca ggagcagcag cgcggtcagc
     3241 cggtgctgga gacgatgcgt catgaacgtg tgcctctcag gtataggctc tcacggagtg
     3301 cgggggtggc gaggggccgt cgacttcggc ggctggcgtg tggggcacgg atccttcgac
     3361 gacgagcaga atgacaagga tagaggatga tctgtacgac gacgggtgtc gtattcgtca
     3421 gccccttctt atctctgtca tcccgaccgc aggggaggga tccgtcttgc cgcatgtcca
     3481 gtcctcgtcg tccgccacga tgtctcaccg cggccagcgc aaccccctcg ccaagccgcc
     3541 acgcagggat cataatcgcg gcgcacggaa ggagcaccga tgatcgatct gaatagtgac
     3601 accgccagcc gcccctcggc cgagatgcgg cagttcatgg cggaagcgcc ggtcggcgac
     3661 gagcagttgg gcgaagatcc ctcggtcaac cagctgcagg acatgacggc cgcgctcttg
     3721 ggcaaggaag ccgcgctctt tctcccctcc ggcaccatgt gcaacgccat cggcatcaag
     3781 gtccataccc agcccggcga ccgcatcgtg ctggagcgct acgcccaccc cttcacctcc
     3841 gagggcgccg gcccggccct gatgtccggc atcatgaccg ccaccgtcga gggggtgcgc
     3901 ggcgtcttcc agcccgagca ggtggccgag atcgcttcgc cggccaacaa ccacgttccg
     3961 cccaccaccc tggtctgcct ggagaacacc cacaaccgcg gcggcggcaa ggtctggccg
     4021 ctggacacgc tcaacgccgt ggcccagacg gcgcatcagc tcggcctcaa gacccatctc
     4081 gacggcgccc ggctgctcaa cgccgccgtc gcctcgggta tcccagccgc cacctgggcc
     4141 gagaatatgg atacggtctg gatcgacctc tcgaaagggc tcggcgcgcc ggtcggcggc
     4201 gtgctggccg gcgatgccga gacgatgcgg cgcgcgcggc gctacaagca catgttcggc
     4261 ggcgccatgc gccaggcggg gatcatcgcg gccggtggga tctacgccct ggagcacaac
     4321 gtcgagcggc tggccgagga ccacgccaac gcgcggcggc tggccgaggg tctggc
//
LOCUS       DASUBI010000621         9454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_19085, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000621 DASUBI010000000
VERSION     DASUBI010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19085"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFU72_11965"
     CDS             <1..427
                     /locus_tag="VFU72_11965"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEU5424251.1"
                     /translation="ERTPMQWDGKPNAGFSSGTPWLPIAEDFREVNVARERDDPRSLL
                     TLHWRLIALRRRETALRVGAYVPRGAYGDLFAYERRYGERRFLITLNLGHQPLVLPLA
                     EHERGVVALSTFLDRAGDALAGQVELRGDEGLVLQLAVR"
     gene            513..860
                     /locus_tag="VFU72_11970"
     CDS             513..860
                     /locus_tag="VFU72_11970"
                     /inference="COORDINATES: protein motif:HMM:NF039991.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6092 family protein"
                     /protein_id="HEU5424252.1"
                     /translation="MTEPRVLTEDEAFRLLAHLIATAELHTIEPPHYAGRRMVEGTLP
                     LLAAMIRDGDERSRDWLRGFQQQVEAALEARRSDAAAFESFLREAPGEIVRELKRRRA
                     SEAAVKEPDRGAA"
     gene            847..1422
                     /locus_tag="VFU72_11975"
     CDS             847..1422
                     /locus_tag="VFU72_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5424253.1"
                     /translation="MAPREQADALYAVMRTRRAVRAFTAEPVSVADIRRILEAARWAS
                     SAGNRRLHRFYVTRDPQQIDLISTVGPGFYGTPAALIVICTDLAKAAQAGVREGSDES
                     TWIDVGTAAMNMQLMAHALGLGSTPVTSVSRSGLLEALELPEHLRPELILQLGHPAPA
                     AVPRTGRGQRLRIEELTDWEQVGGSIPGGSA"
     gene            complement(1665..2444)
                     /locus_tag="VFU72_11980"
     CDS             complement(1665..2444)
                     /locus_tag="VFU72_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU5424254.1"
                     /translation="MTAPSYAVYAIKYAQREARRTEHFFGGDPHDGPMSMDYFVWAAV
                     SAEQTVVIDTGFSATVAARRGRAHLRAPADALALLDIDAAQVPLVILTHLHYDHCGNL
                     ADFPAARFVVQDAALAFWTGRYAGRGVYRPLIEPEDIVALVRANFERRLRFVDGSAEV
                     APGISVHRVGGHARGLQVVRVATASGPLVLASDATHYYENIDEDRPFSLVDELAAMYE
                     AFDTVHALAGPGGPIVPGHDPLVLERFPAVPGLEGIAVRLD"
     gene            2538..3410
                     /locus_tag="VFU72_11985"
     CDS             2538..3410
                     /locus_tag="VFU72_11985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5424255.1"
                     /translation="MSESTAVIGFIGLGRMGSLLARRFVEAGYALIGFDQAGTAERLP
                     PGARAAASTAEVAAESDVVFLSLPDGAASIAVARQLAAAAPRRATTVVDLSTIGIASA
                     RECAAILGGAGIGYVDAPVSGGPAGARDGTLAVMTATEAATFERLRPLLGVFGRNLFR
                     LGDAPGQGQAMKLVNNFLAATNLAAASEAMLFGAREGLDLGQMIDVVNASSGRSAATQ
                     EKFPRSIIPGGYDYGFAAALMAKDVTLYRESVAAVGTADPLGAVVAELWQRFNGACPG
                     VDFTYIYEYLRQGD"
     gene            complement(3793..4152)
                     /locus_tag="VFU72_11990"
     CDS             complement(3793..4152)
                     /locus_tag="VFU72_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424256.1"
                     /translation="MAHELHLVLAAASTMAVLLVALDGLRRALRGVPAGPRSARLFDL
                     SLVLLVITSASGLGLLVGGGRPRELYHLMYGGFALMTLPVVGAFGPRARDRGYGAATA
                     AGALFALVVIARLFATG"
     gene            complement(4174..5367)
                     /locus_tag="VFU72_11995"
     CDS             complement(4174..5367)
                     /locus_tag="VFU72_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5424257.1"
                     /translation="MPIAPPDAPPAEAPPAQPTARPDRAVAPGGGILETIEDQLALRR
                     LIRDYFIPVETNTFWYTLGGVLAIALTLEIFTGVLLSFEYRPDAGQAFAITSRLIHST
                     FWHVIINFHYLNAFLIFGLVMIHMMRVFVSGGYRRGKQGLWLIGVGLAGLTFILSLTG
                     EALHWDEVGFAVPWHISEFFQAIGLANTFGYTFAALKAVPTASAKLSQIYAIHIAVAS
                     ILLLLFLVMHYYLIKEKGISLPFWIKPSGKTAPFSKHIREWLLYSAVILGAVLLLAIF
                     VPRGTGTAPQLLPESPYYGSTHGPGALGAKPSFPISWTHGMNVFFGEHLGIEPDIWGT
                     VVGMTLMTLALLVIPFVDRAPREPANPEQAFDWRQRGWAFAAIAIFWIVLIVGVVQNA
                     IAGSG"
     gene            6015..7712
                     /locus_tag="VFU72_12000"
     CDS             6015..7712
                     /locus_tag="VFU72_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5424258.1"
                     /translation="MAEQLHLILNISMAVAIALAGGLLAHRLRQSVIVGYLLAGIVIG
                     PFSPGFVGDRGQIAALAEVGVIFLMFALGVEFSLKELARVKAVALGGTALQVLLLMAA
                     GLGLGAALGWPFGQALFFGGILAISSTMVILKTLLDRGEVGSRHGRVLLAMLIVQDLA
                     VVVLIVLLPRLAAASGGADLGALLLTLIKAVAFIAVTLFLGVRVVPRLLERVEQIRSA
                     ELFLLTAVALALGAATVSALLGLSVALGAFMGGLMLSESEFDHRVVAEVVPMRDLFAT
                     LFFVSVGMLIDPAFALRHLPAVVGLALFIMAAKAVLTLVALLPFGLSGKTAAFTSLGM
                     IQIGEFSYVLANAGRAAGAISDELNSLVLTASVLTIVLTPLAFWVAPRVGVWLGRQPL
                     LERAFSDRRAARGLEELFHGHAVVIGYGRVGRSVAQGLRESGLTVVVIEEDLRRVREA
                     QDVGLTVVYGDASYNSVLRAARVGNSGLVAVALPDSGATRVVVRYSRRANRTVPIVVR
                     LAREEDTALLLRLGASAVVAPERAGARLMLEVSERALGMPTGAIEAMAGEFPTALEG"
     gene            7804..8475
                     /locus_tag="VFU72_12005"
     CDS             7804..8475
                     /locus_tag="VFU72_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424259.1"
                     /translation="MNILVVEDEQRLARLLKRMLEDERHTVDVAYDGGTALELGLRDS
                     FDLAVLDRMLPDLDGIEVCRRLRAARIAMPILMLTARGSVEDRVTGLNAGADDYLVKP
                     FAMDELLARVNALLRRGGSNGAATVLQVGDLTLDLLRREVKRGGAIIELTPKEFALLE
                     YLMRNPGHVLSRSQIVDHVWSYDFDALSNVVDIYIHYLRDKIDRGRGKPLIKTVRGAG
                     YRIEG"
     gene            8540..9298
                     /locus_tag="VFU72_12010"
     CDS             8540..9298
                     /locus_tag="VFU72_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424260.1"
                     /translation="MTSFSWGAAEAARLDAIRESLAQVSTASAFQLLNDLGWRNTHMQ
                     GLRPIEPLGLGRRLVGRARTCRYLMRREPQQPAAPGGSRRAPSPVIALIESIEPGDVL
                     CVDALGVPTAGIIGDILAARLKARGAVAALVDGAVRDTPYIKQVGLPVFTAAIHPAAS
                     MRDVVPIDYDRPVNLGGAQVIPGDVILADDEGALAMPLELAEYVAEQGRAKELLEEWI
                     RGKIEAGGAVPDYYPPSPEKAREYERETGRSAQH"
BASE COUNT         1443 a         3134 c         3394 g         1483 t
ORIGIN      
        1 ggagcgcacc ccgatgcagt gggacggcaa gccgaacgcc ggcttcagca gcggcacgcc
       61 ctggctgccg atagctgaag acttccggga ggtcaacgtg gcgcgcgagc gcgacgatcc
      121 gcgttcgctg ctgactctgc actggcggct gatcgcgctg cgccggcggg agacggccct
      181 ccgcgtgggc gcgtacgttc cccgtggcgc ctacggtgat ctcttcgcct acgagcgccg
      241 atatggtgag cgccgcttcc tgatcaccct caacctgggt caccagccgc tcgtcttgcc
      301 actcgccgaa cacgagcgtg gcgtcgtcgc gctctccacg ttcctcgacc gggcgggcga
      361 cgcgctcgcc gggcaagtcg agctacgcgg cgacgagggg ctggtgctcc aactggccgt
      421 ccggtgagca aacgggcccg caccggtcgc gattgctccc agcacgccgc aatgagacaa
      481 tgcccggcgc gatgggggaa ggatgggagt cgatgaccga gccgcgcgtg ctgaccgaag
      541 acgaagcgtt tcgcctgctg gcccacctga tcgcgacggc ggagctgcac accatcgagc
      601 cgccgcacta cgccgggcgg cgcatggtcg aggggacgct gccgttactc gctgccatga
      661 tccgcgacgg cgacgagcgg agccgtgatt ggctgcgggg cttccagcag caggttgagg
      721 cggcgctgga agcccggcgc agcgacgccg ccgcgttcga gtcgtttctg cgtgaggcgc
      781 cgggcgagat cgtgcgcgag ctcaagcggc ggcgggcgag tgaggccgcc gtgaaggagc
      841 ccgaccgtgg cgccgcgtga gcaggcggac gcgctctacg cggtgatgcg cacccgccgc
      901 gcggtgcgcg cgtttaccgc cgagccggtt tccgtagccg acattcgccg catcctcgaa
      961 gcggcgcgct gggcctccag cgccggcaac cggcgactgc accgcttcta cgtgacgcgc
     1021 gatccccagc agatcgatct catcagcacc gtcggccccg gcttctacgg cacgcccgcc
     1081 gccctgatcg taatctgcac cgacctcgcg aaggcggccc aggcgggtgt gcgcgagggc
     1141 agcgacgaga gcacctggat cgacgtcggg acggcggcga tgaacatgca gctgatggcg
     1201 cacgccctgg gcctcggctc gacgccggtc acctcggtca gccgctcggg gttgcttgaa
     1261 gcgctcgagc tgccggagca cctgcgcccc gagttgatct tgcagctcgg ccacccggcg
     1321 cccgcggcgg tgccgaggac aggccgcggc caacggctgc ggatcgagga gctcaccgac
     1381 tgggaacagg ttggcggttc catccccggc gggagcgcct agcgcggttg gcacagggat
     1441 tatccatggt aggggcagac ccctgtgtct gccttcgccg ggccatcgcc gattctcgtc
     1501 gttcgcgcca cggtggcccc gttcccagtc gccacggcgg agcggcaaca gggcagacac
     1561 aggggtctgc ccctacaggg aacgcgccgc gccagccgtg gacaaggtaa tacggtgtag
     1621 cgcgtcaata ctattgtcga ccggtgcatc cactccagct cgcctcagtc aaggcgcacc
     1681 gcgattccct ccaggcccgg gacggccggg aagcgctcca gcaccagcgg gtcgtgcccc
     1741 ggcacgatcg ggccaccggg accggccagc gcgtgtacgg tatcgaaggc ctcgtacatg
     1801 gcggccagct cgtcgaccag gctgaagggc cggtcctcgt cgatattttc ataatagtgg
     1861 gtggcgtcgg aggcgagcac gagcggaccg ctcgcggtgg cgacccgcac cacctgcagg
     1921 ccgcgcgcgt gtccgccaac ccggtggacg ctgatccccg gcgcgacctc ggcgctgccg
     1981 tcgacgaagc gcagcctgcg ctcgaagttg gcgcgcacca gagccacgat gtcctccggc
     2041 tcgatcaggg ggcggtagac gccgcgcccg gcgtagcggc cggtccagaa ggcgagcgcg
     2101 gcgtcctgca ccacgaagcg ggccgctggg aagtccgcca ggttgccgca gtggtcgtag
     2161 tggaggtggg tcaggatcac gagaggcacc tgggcggcgt cgatgtcgag cagggccagc
     2221 gcgtcggcgg gggcgcgcag gtgggcgcgc ccgcggcgag cggcgaccgt ggcgctgaag
     2281 ccggtgtcga tgaccacggt ctgctcggct gagacggcgg cccagacgaa gtagtccatc
     2341 gacatcgggc cgtcgtgcgg atcgccgccg aagaagtgct cggtgcggcg cgcctcgcgc
     2401 tgggcgtact tgatggcata aaccgcgtag ctcggcgccg tcatcgcccc tgctccttcc
     2461 attgcttgta caccggcacc gacgtagagg atactgccgg tatcgggaac gcgcgagcgg
     2521 acgagaggac ggctgtcatg agcgagagca ccgcggtgat cggcttcatc gggctcgggc
     2581 gcatggggag cctgctggcg cgccgcttcg tcgaggccgg ctacgcgctg atcggcttcg
     2641 accaggctgg cacggccgag cgcctgccgc ccggcgcgcg ggcggcggcg agcacggccg
     2701 aggtggcggc cgagagcgac gtcgtcttcc tcagcctgcc cgacggcgcg gcctcgatcg
     2761 cggtcgcacg ccagctggct gccgccgcgc cgcgccgtgc gacgaccgtg gtcgacctct
     2821 cgaccatcgg catcgcctcc gcccgcgagt gtgccgcgat cctcggcggg gccggaatcg
     2881 gctacgtcga cgcgccggtc agcggcgggc cggccggggc gcgcgacggg acactcgcgg
     2941 tgatgaccgc gaccgaggca gccaccttcg agcggctgcg gccgttgctg ggcgtcttcg
     3001 gtcgcaacct ctttcgcctg ggtgacgcgc ccgggcaggg gcaggcgatg aagctggtca
     3061 acaacttcct ggccgcgacg aatctggcgg cggcctcgga ggcgatgctc tttggcgcgc
     3121 gggaggggct cgatctaggg cagatgatcg acgtggtgaa cgcctccagc ggacgcagcg
     3181 ccgcgacgca ggagaaattc ccgcgcagca tcatccccgg cggctacgac tacggcttcg
     3241 ccgccgcgct gatggccaag gacgtgaccc tctaccgcga gagtgtggcg gcggtcggca
     3301 cggcagaccc gctcggcgcg gtggtggccg agttgtggca gcgcttcaac ggcgcctgcc
     3361 ccggcgtcga cttcacctac atctacgagt acctgaggca gggagattag ccaaggctca
     3421 tccgtcgggt tgagcacgcc gcgagggccg cgctgtggta gggatgaacc attcaccgca
     3481 gcggcactgc gtcgtgctgt ggctcgctgc cctggatcgg gctgcgtgtg gagctgccgg
     3541 tcaggctatc cactaatgaa tcgacgccgg cgggcgcctg gacacgcttc tcgaatggcc
     3601 agccaccatg ctgggcgtct gcgtttccgc ctcaatcagc ctcaggtttg gagcggtcct
     3661 gctggcggcg ggcagggtcg cggtggccac ctacgccgag atctggcggc gtccccccaa
     3721 gccggcggcc cgaccgatcc ggttgttggg aggcgccgac ccgtcgttgc tcggtgaaac
     3781 ggcggtaccg cctcagcccg tcgcgaagag gcgcgcgatc acgaccaagg cgaagagagc
     3841 gcccgcggcg gttgcggctc catacccgcg atcccgcgca cgcggcccaa acgcccccac
     3901 gaccggcagc gtcattaagg caaagccgcc atacatcaga tggtagagct cgcggggacg
     3961 tccgccaccg accagcaaac ctaaccccga agcgctggtg atcaccagca ggaccagcga
     4021 caagtcgaag aggcgggccg agcgcggccc ggcgggcacg ccgcgcagcg cgcggcgcag
     4081 cccatcgagc gctaccaaca gcaccgccat cgtactggcg gccgccagta ccagatgcag
     4141 ttcgtgcgcc atgctcgtct caggctcccc gcgctaccca cttcccgcga tcgcgttctg
     4201 caccacgccg acgatcaaca cgatccagaa gatcgcgatc gccgcgaacg cccagccccg
     4261 ttgccgccag tcgaacgcct gctcgggatt cgccggctcg cgcggcgcgc ggtcgacgaa
     4321 ggggatcacg agcagcgcta gtgtcatcag cgtcatgccg acgacggtgc cccagatgtc
     4381 cggttcgatg ccgagatgct cgccgaagaa gacgttcatg ccgtgagtcc agctaatcgg
     4441 aaacgaaggc ttggcgccca gcgcgccggg tccgtgggtc gagccgtaat agggcgactc
     4501 cggcagcagt tggggcgcgg tccccgtgcc gcgcggcacg aagatggcca agagcagcac
     4561 cgcgccgagg atcaccgcgc tgtagagcag ccactcacgg atgtgcttgc tgaagggcgc
     4621 cgtcttgccg ctcggcttga tccagaaggg gagggagatg cccttctcct tgatcaggta
     4681 gtagtgcatc accaggaaga ggagcagcag gatcgacgcg acggcgatgt gaatcgcgta
     4741 gatctgactg agcttggcgc tggcggtcgg cacggctttc aaggcggcga aggtgtagcc
     4801 gaaggtgttg gccaggccga tcgcctggaa gaactccgag atgtgccagg ggaccgcgaa
     4861 gccaacctca tcccagtgca gcgcttctcc agtcaaggag aggatgaagg tcagcccggc
     4921 caagccgacg ccgatcagcc atagcccctg cttgccgcga cggtagccgc cgctgacgaa
     4981 gacccgcatc atgtggatca tgaccaagcc gaagatcagg aaggcgttga ggtagtggaa
     5041 gttgatgatg acgtgccaga aggtggagtg gatcaagcgg gaggtgatgg caaaggcttg
     5101 gccggcatcc ggacggtact cgaaggagag gagcacgcca gtaaagatct cgagggtgag
     5161 ggcaatggcc agcaccccgc cgagcgtgta ccaaaaggtg ttggtctcga ccggaatgaa
     5221 atagtccctg atcagccggc gcagggccag ttggtcctcg atggtctcca ggatgccgcc
     5281 acctggagct accgccctat ccggcctcgc ggtgggctga gcaggtggag cttccgccgg
     5341 aggggcgtcg ggcggcgcga ttggcattga atgcatttcg gacgacatca ggaacccctt
     5401 cctgtgactg tcgcgccaac acatccgcta taactacggc atcgatgtgc ccatcatagc
     5461 aaagacgcgc gccaatgact agcgcatgcg ggagtcctta cgggcgtatt acgatacact
     5521 cgataggccg tgggctggct cagcgaatcg cgctggatgg caagtagatc gccgagttga
     5581 tcaagcggtg cgccagggca cggcgtctcg ccacctgcgc cggctctctg ggcgccgaag
     5641 tgatggatag catgctgtcg gggacggtcg ccgcgctgcg tgagccggcc ggcacagagg
     5701 cgggccactg gcgtcgattc gtcagttggt ggctggaccg cccgctctac gcgctgcccg
     5761 atccagggca gcgagccaca gcgcggcgca gcgccactgc ggcggacagt tcacccccca
     5821 ccgcggcgcg gacccgcacg gcgtgctcaa cccggcggat gcccctcggc tcgctcggcc
     5881 ctgcgcgcgt tctttggctc ggagtgacgc ttcccctggt ttcttcgcgg attttccgct
     5941 ttacttcatt actagtacag gctgtgccgc gccccgaccc cggcgcgacg gtgccgctcc
     6001 cgagagggcc ttcagtggcc gaacaattgc acctgatcct gaacatcagc atggcggtcg
     6061 ccatcgcgct ggctggcggg ttgctggcgc accggttgcg ccaatcggtg atcgtcggct
     6121 atctgctggc cgggatcgtg atcggcccct tcagccccgg cttcgtcggc gaccgcggcc
     6181 agatcgccgc cctggccgag gtgggcgtga tctttctgat gttcgcgctc ggcgtcgagt
     6241 tctcgctcaa ggagctggcg cgcgtcaagg cggtggcgct gggcggcacg gcgctccagg
     6301 tgctgctctt gatggccgcc ggcctggggc tgggggcggc tctcggctgg ccgttcggcc
     6361 aggctctctt cttcggcggc atcctcgcca tttcgtcgac catggtgatc ctcaagacgc
     6421 tgctcgaccg gggcgaggtc ggctcgcgcc acggccgcgt gctgctggcg atgctgatcg
     6481 tgcaggacct ggcggtagtg gtgctgatcg tcctcctgcc gcggctggcc gccgccagtg
     6541 gcggcgccga tctcggcgcg ctgttgctga cgcttatcaa ggcagtcgcc ttcatcgccg
     6601 tcaccctctt cctcggcgtg cgcgtggtgc cccggctgct ggagcgcgtc gagcagattc
     6661 gctccgccga gctcttcctg ctgaccgccg tggcgctggc gctcggcgcc gccacggtca
     6721 gcgcgctgct gggactttcg gtggcgctgg gcgccttcat gggcgggctg atgctcagtg
     6781 agtcggagtt cgaccatcgg gtggtggctg aggtggtgcc gatgcgcgat ctcttcgcca
     6841 ctctcttctt cgtctcggtc ggcatgctga tcgaccccgc cttcgcgctg cgccacctgc
     6901 cggccgtggt cggactggcg ctcttcatca tggccgccaa ggcggtgctg accctggtcg
     6961 cgctgctccc cttcggtctc agcggcaaga ccgcggcctt caccagcctc gggatgattc
     7021 agatcggtga gttcagctac gtgctggcca atgccggacg ggcagcgggc gccatctcgg
     7081 acgagctgaa cagcctggtg ctgaccgcct cggtgctgac gatcgtgctg acgccgctgg
     7141 ccttctgggt ggcgccgcgg gtgggggtct ggttaggtcg ccagccgctg ctggagcgtg
     7201 ccttctcaga ccggcgcgcg gcgcgcgggc tggaagagtt gttccacggg cacgccgtgg
     7261 tcatcggcta tggtcgagtc ggccgctccg tcgcacaggg gctgcgcgag agcgggctga
     7321 cggtggtggt gatcgaggaa gacctgcgcc gggtgcgtga ggcgcaagac gttggtctga
     7381 cggtcgtcta tggagatgcc tcctacaata gcgtgctgcg cgcggcccgg gtgggcaact
     7441 ccgggctggt cgcggtggcg ttgcccgact cgggagccac gcgcgtggtg gtgcgctaca
     7501 gccggcgcgc caaccgtacg gtgccgatcg tggttcggct ggcacgggag gaggacaccg
     7561 cgctgctgct gcgactcggc gccagcgcgg tcgtcgcacc cgagcgcgcg ggggcgcggc
     7621 tgatgctcga ggtcagcgag cgggcgctcg gcatgcccac cggtgcgatc gaggcgatgg
     7681 ctggcgagtt tccgacggcg ctggaaggct gacgcccgat tttctcatct ttctctcatc
     7741 ttcgggccgt aaggttgccc cggcgtcacg gagtcaggca cgggggtgcc cgaggagtcg
     7801 acgatgaaca tactggttgt cgaggatgag cagcgcctgg cacgcctgct caagcgcatg
     7861 ctcgaggacg agcgccatac ggtcgacgtg gcctacgacg gaggaacggc gctggagctg
     7921 ggcctacgcg acagcttcga cctggccgtg ctcgaccgca tgctgcccga tctcgacggg
     7981 atcgaggtct gccggcggct gcgtgcggcg cggatcgcca tgccgatcct gatgctgacg
     8041 gcgcgcgggt cggtcgagga ccgggtgacc ggcctcaatg ccggggccga cgactacctg
     8101 gtcaagccct tcgccatgga cgagctgctg gcccgcgtca acgcgctgct gcggcgcggc
     8161 ggcagcaatg gggccgcgac ggtgttgcag gtgggtgacc tgacgctcga cctcttgcgg
     8221 cgcgaggtca aacgcggcgg ggcgatcatc gagctgaccc ccaaagagtt cgccctgctg
     8281 gagtacctga tgcgcaaccc cggccacgtg ctgagtcgca gccagatcgt cgaccacgtc
     8341 tggagctacg acttcgacgc gctctccaac gtggtcgata tctacatcca ctacctgcgg
     8401 gacaagatcg accgcggccg cggcaagccg ctgatcaaga ccgtgcgcgg cgccggctac
     8461 cgcatcgaag ggtgaggtct ctccatactc gcgctacaat cgccgcgttg gttcctggcc
     8521 gacgggaagg gagcgcggca tgacgagttt ttcctgggga gcggcggagg cggctcggct
     8581 agacgccatc cgcgagtcgc tggcgcaggt cagcacggcc agcgccttcc agctactcaa
     8641 cgacctcggc tggcgcaaca cgcacatgca ggggctgcgg ccgatcgagc ccctggggct
     8701 tggccggcgc ctggtcggcc gggcgcgtac ctgccgctac ctgatgcggc gggagcctca
     8761 gcagcccgcg gcacccggcg gcagccgccg cgctccctcc ccggtgatcg cgctgatcga
     8821 gtcgatcgag ccgggcgacg tcttatgtgt cgacgcgctc ggcgtgccga cggccggcat
     8881 catcggggac attttggcgg cccggctcaa ggcgcgtggc gcggtggcag ccctggtcga
     8941 cggcgctgtg cgcgacacac cgtatatcaa gcaggtcggg ctgccggtct tcaccgccgc
     9001 catccacccg gctgccagca tgcgcgacgt agtccccatc gactacgacc ggccggtcaa
     9061 cctcggcggc gcgcaggtca tccccggcga cgtcatcctg gccgacgatg agggtgcgct
     9121 ggcgatgccg ctggagctgg cggagtacgt ggccgagcaa gggcgggcca aggagctact
     9181 ggaggagtgg atccgcggca agatcgaggc cggcggcgcg gtgcccgact actacccgcc
     9241 atcgcctgag aaggcgcgcg aatacgagcg cgagacgggt cgcagcgcgc agcactgagc
     9301 cggctgagcg atcgagcgga ggctgatcgg gagcgatcgg gaaggggaac ggcatgattg
     9361 ggttggtcgt ctcggtgcgg gtgcggccgg agcggcggga ggagttcctg cgcgcggccg
     9421 aggaggacag tcgcggctcg cgcgaggacg agcc
//
LOCUS       DASUBI010000622         6575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_99737, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000622 DASUBI010000000
VERSION     DASUBI010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99737"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFU72_12015"
     CDS             <1..572
                     /locus_tag="VFU72_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5424261.1"
                     /translation="LNAYADRLLLLRGSGVYTDLAALAAELVTLRDEGGSAPCVLFLD
                     YLQKVPVNPEPANETERVTRVVDGLKELALSLGLPVVAIVAADNDGLRAPRLRLHHLR
                     GGSAILYEADIILILNEKHRIVAKQHLTYNEHDAKSFHAWIVCSIEKNRAGRQLVDLE
                     FQKHFEYACFDPDGRPVGEMLIDERIERE"
     gene            603..1331
                     /locus_tag="VFU72_12020"
     CDS             603..1331
                     /locus_tag="VFU72_12020"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU5424262.1"
                     /translation="MIVAGLLVSLLLGLLLGPPLNLLIDRLPRRLALWRGPACLSCGQ
                     TRPARAWLPLAGWLLAHGRCERCGTPLPRRALAVEAALPLAAGALWLRDGGVARFPLD
                     LLLAAYFIAIVAIDVEHRLVLNRMTGAGLLAALGIALVGLGPSLPAALLGATVGFVFL
                     FIPALLLPGLGMGDVKLAAVIGALVGFPAVFDALALGVVAGGLAAALLLATRRVGRRG
                     TMAYAPYLVVGVALVLFGLVGGGR"
     gene            1334..2260
                     /locus_tag="VFU72_12025"
     CDS             1334..2260
                     /locus_tag="VFU72_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424263.1"
                     /translation="MSGRRARKPLIEFLTLLALFPLLAGCASGFGGARYLPADAGVDP
                     FSTVRTRAQQYYQAGQTSEEKGDWRKALDDYQQARLWDPDSRQDIADAIGRAQSQLDL
                     RAKPPATPPPTPRPVLPHPSAPTPEPTAAPNAPHAQPTPTPRPAATGAALAGMRAFHS
                     KNFPYSISYPSNWLAKEGDAQGSSSDTFLGEPAKGTGALVIVSMEQVDPRVTLETLYA
                     ATAQVLQAGGAQNVQVAERRQVGGQPAYVLSYRASSDSGQVTARHAIFLTPGFAWHVV
                     LLATPTTTPALLDTFTAMLDSLQLAPGAFPTQ"
     gene            2305..3315
                     /locus_tag="VFU72_12030"
     CDS             2305..3315
                     /locus_tag="VFU72_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424264.1"
                     /translation="MARVEPQERARARVWGLAQAKCDRRTLLLGLAAFSLAACGGAQA
                     VDVPTLPSSPPQTPTPAVAATPTPIVMPTQSSLPLDDKGFADPAFRALWQRTDGAVAS
                     GKVTRAELWGQPVPFGARREPYAEAKGGERLVQYFDKGRMEINDPTADPKSEWYVTSG
                     LLPEEMVSGRVQVGDGTYQTLEPAQLPVTGDLESPSPQTPRYADYNGARRDPVANKVG
                     QAVTTKFVRGASDQEITPPTPVKNASFDQTLGHNIPDVFMNYFNGDLAGMGLNWLFVM
                     GHPLSEPYWITINAGDKTETVLVQLFERRALSYTPAADGSWQVEFTNIGLHYYRWRYH
                     DR"
     gene            complement(3325..6471)
                     /locus_tag="VFU72_12035"
     CDS             complement(3325..6471)
                     /locus_tag="VFU72_12035"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEU5424265.1"
                     /translation="MAGFTESVVEDAALEWLRQLDYEILYGPDIAVDGRAPERSDYHD
                     VLLRGRLSDALTRLNPHASLEAREEVVRRVGQIGSPLLVQANRAFHKLLVDGIKIEVL
                     KDGEPRGERIVLIDFDEPANNEWLAVNQFTVVDGRIERRPDIVIFVNGLPLAVVELKN
                     TADESATIEQAYNQLQTYQAQIPRLFHSNELLVISDGAKTEIGCITTPRERFAAWKTI
                     DGDELLPSGTLETAIKGVFEHRRFLDLIRSFIVFEDDGKTITKKVAHYHQFHAVRKAL
                     ATALRASSVQGDGKGGVLWHTQGSGKSLTMLFFAGKLIAHPALANPTIVMLTDRTDLD
                     DQLHKTFLHGREILRQEPIRAESRAELRQLLSVNAGGVVFTTIQKFMPEPGEEDYPLL
                     SERRNIIVMADEAHRSQYGFGDRIGESGKFVRGFAQNMHDALPHATFVAFTGTPLELA
                     DRDTRVVFGDYIDIYDVARAIADGATVPIYYESRLIKLDLPSDQASLLDEEFDEITEG
                     EEAKRRDKLASRWSQLEAVVGTPKRLAEVAGDLVEHIERRQEALDGKVMIVCMSRRIC
                     VDLYDQLITLRPEWHGEGDASGQLKVIMTGSASDPVEWQQHIRNKERREVLADRFKKA
                     DDPFRFVIVRDMWLTGFDAPSLHTIYIDKPMRGHGLMQAIARVNRVFHDKPGGLVVDY
                     LGIANNLKAALRAYMREDPSGGKPIENEELDIGELVAAMIEKLELCRETFRGFNYGAF
                     LTGSPSERLDIISNAEDFLIRRNYRERGADVIGRFLDHATALLKAFALVSATREAQLV
                     KTEVAFFQTIKAALIKTTGRGSAAVDVNLDHAIRQLVDQAIAPEGVVDIFAAAGLEKP
                     DISILSDGFLAEIRDMPQRNLALELLRKLLNDEISATKRTSVVQARRFSEKLEESLNR
                     YHNRALETAQIIEALIDLARDLREANARGESLGLSQDEVAFYDALGANESAVEVLGDA
                     QLRIIAREVAETVRDNVTIDWTVRENARANLRRLVRRILRRHGYPPDQQEPATLLVIE
                     QAELFAREAATPEPLYRG"
BASE COUNT          965 a         2280 c         2276 g         1054 t
ORIGIN      
        1 gcctgaacgc ctacgccgac cgactgctcc tcttgcgcgg ctcgggcgtc tacaccgacc
       61 tggccgcgct ggccgccgag ttggtcacgc tgcgcgacga gggcggcagc gctccctgcg
      121 tcctcttcct cgactacctg cagaaggtgc cggtcaaccc cgagccggcc aacgagaccg
      181 agcgcgtgac gcgggtggtc gacgggctga aggagctggc gctctcgctg gggctgccgg
      241 tggtggcgat cgtggcggcg gacaacgacg ggctgcgcgc cccccgcctg cggctgcacc
      301 acctgcgcgg cggctcggcc atcctctacg aggccgacat catcctgatt ctcaacgaga
      361 agcaccgcat cgtcgctaaa cagcacctga cctacaacga gcacgacgcc aagagcttcc
      421 acgcctggat cgtctgctcg atcgagaaga accgcgccgg gcggcagttg gtcgacctcg
      481 agttccagaa gcacttcgag tacgcctgct tcgatcccga cggccgcccg gtcggcgaga
      541 tgctgatcga cgagcgcatc gagcgtgaat agggagtaga tggagatggg gggaaggtcg
      601 cggtgatcgt cgcggggctg ctcgtgagct tgctcctggg gctgctgctc ggcccgccgc
      661 tcaatctgct gatcgaccgc ctgccgcgcc gcctggcgct ctggcgcggc ccggcctgcc
      721 tgagctgcgg gcaaacgcgg cccgcccgcg cctggctgcc gctggcgggc tggctgctgg
      781 cccacggccg ctgcgagcgc tgcggcacgc cgctgccgcg ccgggcgctc gcggtcgagg
      841 cggcgctgcc gctggcggcc ggcgcgctct ggctgcgcga cggcggtgtg gcgcgcttcc
      901 cgctcgatct gctcttggcc gcctacttca tcgccatcgt ggccatcgac gtggagcacc
      961 ggctggtgct caaccgcatg accggcgccg ggctgctggc cgcgctcggc atcgccctcg
     1021 tgggcctggg gccgtcgctc cccgccgcgc tgctcggggc gaccgtaggg ttcgtcttcc
     1081 tcttcatccc cgcgctgctc ttgccggggc tgggcatggg cgacgtcaag ctggcggcgg
     1141 tgatcggcgc gctggtcggc ttccccgccg tcttcgacgc gctggcgctg ggcgtggtgg
     1201 ccggcgggct ggcggcggcg ctgctgctgg cgacgcggcg cgtcggccgg cgcggcacga
     1261 tggcctacgc gccctatctg gtggtcgggg tggcgctggt cctcttcggc ctcgtcgggg
     1321 gagggcgcta gccatgtcgg ggagacgcgc caggaaaccg ctgatcgaat tcctcacgct
     1381 gctggcgctc ttcccgctgc tagcgggctg cgcctcgggc ttcggcggcg cgcgctacct
     1441 gccggccgac gccggggtcg accccttcag caccgtgcgc acgcgcgcgc agcagtatta
     1501 tcaggcgggc cagacctcgg aggagaaggg cgactggcgc aaggcgctgg acgactacca
     1561 gcaggcgcgc ctctgggacc ccgacagccg ccaggacatc gccgacgcca tcggccgcgc
     1621 gcagtcgcag ctcgacctgc gggccaagcc gcccgcgacg cccccgccga cgccgcgtcc
     1681 ggtcctgccg caccccagcg cgcccactcc ggagccgacc gcggcgccca acgcgccgca
     1741 cgcgcagccc acgcccaccc cgcgcccggc ggccaccggc gccgctctgg ccggcatgcg
     1801 cgccttccac tccaagaact tcccctacag catcagctac cccagtaact ggctggccaa
     1861 ggagggcgac gcgcagggga gcagctccga caccttcctg ggcgagcccg ccaaggggac
     1921 cggggcgctg gtgattgtca gcatggagca ggtcgacccg cgcgtgacgc tcgagacgct
     1981 ctacgccgcc accgcgcagg tgctgcaggc cgggggcgcg cagaacgtgc aggtcgccga
     2041 gcggcggcag gtgggcgggc agccggccta cgtgctcagc taccgcgcca gcagcgactc
     2101 gggccaggtg acggcgcgcc acgccatctt cctgacgccc ggcttcgcct ggcacgtggt
     2161 gctgctggcc acgccgacca ccacgcccgc cctgctcgac acctttacgg cgatgctcga
     2221 ctcgctgcaa ctggcgccgg gcgccttccc gacgcagtag cgttcgccgc ggcccggccg
     2281 gcacgagaga gtgagacgag agcgatggca agggtggagc cacaggagcg ggcgcgggcg
     2341 cgggtgtggg ggttggcaca ggcgaaatgc gatcggcgca cgctgctgct gggtctggcg
     2401 gcgttcagcc tggccgcctg cggcggggcc caggcggtcg acgtgcccac gctcccctcg
     2461 tcgccgccgc agaccccgac gcctgccgtg gcggccacgc cgaccccgat cgtgatgccg
     2521 acgcagtcct cgctgccgct cgacgacaag ggcttcgccg acccggcctt ccgcgctctc
     2581 tggcagcgca ccgacggcgc tgtcgccagc ggcaaggtca cgcgcgccga gctctggggc
     2641 cagccggtcc ccttcggcgc gcgccgcgag ccctacgccg aggccaaggg cggcgagcgg
     2701 ctggtgcagt acttcgacaa ggggcgcatg gagatcaacg atcccacggc ggatccgaag
     2761 tcggagtggt acgtgaccag cggcctgctg ccggaggaga tggtcagcgg gcgggtgcag
     2821 gtcggcgacg gtacctacca gacgctggag ccggcgcagc tgccggtgac cggcgacctg
     2881 gagagcccca gcccgcagac gccgcgctac gccgactaca acggcgcgcg ccgcgacccc
     2941 gtggcgaaca aggtcggcca ggcggtgacg accaagttcg tgcgcggcgc cagcgaccag
     3001 gagatcacgc cgcccacgcc ggtgaagaac gccagcttcg accagacgct ggggcacaac
     3061 atccccgacg tcttcatgaa ctacttcaac ggcgacctgg cgggcatggg cctgaactgg
     3121 ctcttcgtga tgggccaccc gctcagcgag ccctactgga tcaccatcaa cgccggcgac
     3181 aagaccgaga ccgtgctcgt gcagctcttc gagcgccgcg ccctcagcta cacccccgcc
     3241 gccgacggca gctggcaggt cgaattcacc aacatcggcc tccactacta ccgctggcgc
     3301 taccacgacc ggtagtgttg gcgcttatcc tcgatagagc ggttccggcg tggcggcctc
     3361 gcgggcgaac agctctgcct gctcgatcac aagcagcgta gccggctcct gctgatcggg
     3421 cgggtagccg tggcggcgca ggatgcggcg cacaaggcgg cggaggttgg ctcgggcatt
     3481 ctcgcggacc gtccagtcga tggtgacgtt gtcgcgcacg gtctccgcca cctcgcgagc
     3541 gatgatccga agctgcgcat cgcccagcac ctcgaccgcg ctctcgttgg cgcccagcgc
     3601 atcgtagaac gcgacctcgt cctgcgagag accaaggctc tcgccgcgcg cgttggcctc
     3661 gcgcagatcg cgagcgaggt cgatcagcgc ctcgatgatc tgcgcggtct ccagcgcccg
     3721 gttgtggtag cgattgagcg actcctccag cttctccgag aagcgccgcg cctgcaccac
     3781 gctggtgcgc ttggtggcgc tgatctcgtc gttgaggagc ttgcgcagca gctccagcgc
     3841 gagattgcgc tgcggcatgt cgcggatctc ggccaggaag ccgtccgaga ggatcgagat
     3901 gtccggcttt tcgagcccgg cggcggcgaa gatgtcgacg acgccctcgg gcgcgatcgc
     3961 ctgatcgacg agctggcgga tggcgtggtc gagattcaca tcgacggcgg cactgccgcg
     4021 gccggtcgtc ttgattagcg cggccttaat cgtctggaag aacgcgactt ccgtcttgac
     4081 caactgcgcc tccctggtcg cactgaccag cgcgaaggct ttgagcagcg cggtggcatg
     4141 gtcgaggaag cggccgatca cgtccgcgcc gcgctcccgg taattccgcc ggatgagaaa
     4201 atcctcggcg tttgagatga tatcgagccg ctcggacggc gaccccgtga ggaaggcgcc
     4261 gtagttgaag ccgcgaaacg tctcgcggca cagctccagc ttctcgatca tcgccgcgac
     4321 gagctcgccg atatcgagct cctcgttctc aatcggcttg ccgccgctcg ggtcctcgcg
     4381 catgtaggcg cgtagggcgg ctttcaggtt gttggcgatg ccgaggtagt cgacgaccag
     4441 cccgccgggc ttgtcgtgga agacgcggtt gacgcgggcg atcgcctgca tcagcccgtg
     4501 gccccgcatc ggcttgtcga tgtagatcgt gtgcagcgag ggcgcgtcga agccggtcaa
     4561 ccacatgtcg cgcacgatca cgaagcggaa gggatcgtcg gccttcttga agcgatcggc
     4621 gagaacctcg cggcgctcct tgttgcggat atgctgctgc cactcgaccg ggtcgctggc
     4681 cgagccggtc atgatgacct tcagctgccc gcttgcgtcg ccctcgccgt gccactcggg
     4741 ccggagcgtg atgagctgat cgtagagatc gacgcagatc cgccgcgaca tgcagacgat
     4801 catgaccttg ccgtcgagcg cctcctggcg gcgctcgatg tgctcgacca gatcgccggc
     4861 gacctcggcc agccgcttgg gcgtgccgac gaccgcctcc agctgcgacc agcgcgaggc
     4921 gagcttgtcc cggcgcttgg cctcctcacc ctcggtaatc tcgtcgaact cttcatcgag
     4981 gaggctggcc tgatccgacg gcaaatcgag cttgatcagc cgggactcgt agtagatcgg
     5041 cacggtcgcg ccgtcggcga tcgcgcgcgc gacatcgtag atgtcgatgt agtcgccgaa
     5101 gacgacgcgt gtgtcgcggt cggccagctc cagcggcgtg ccggtgaagg cgacgaaggt
     5161 cgcgtgcggc agggcgtcgt gcatgttctg cgcgaagccg cgcacgaatt tcccgctctc
     5221 gccgatgcga tcgccgaagc cgtactgcga gcggtgcgcc tcgtcggcca tcacgatgat
     5281 gttgcggcgc tcgctgagca gcgggtaatc ctcttcgccc ggctcgggca tgaacttctg
     5341 gatggtggtg aagacgacgc ccccggcgtt gacgctgagt agttggcgca gctcggcgcg
     5401 cgactcggcg cggatcggtt cttgccgcag gatctcccgg ccgtgcagga aggtcttatg
     5461 cagctggtcg tcgaggtccg tgcgatcggt gagcatcacg atggtcgggt tggccagcgc
     5521 cgggtgcgcg atcagcttgc cggcgaagaa gagcatggtc agcgacttcc ccgagccctg
     5581 cgtgtgccag agcacgccgc ccttgccgtc gccctggacc gacgacgccc gcagcgccgt
     5641 cgccagcgct ttgcggaccg cgtggaactg gtgatagtgc gcgaccttct tagtgatggt
     5701 cttgccgtcg tcctcgaaga cgatgaagga gcggatcagg tcgaggaagc ggcggtgctc
     5761 gaagacgcct ttgatggccg tctccagcgt gccgctcggc aggagttcgt cgccgtcgat
     5821 cgtcttccag gcggcgaagc gctcgcgcgg cgtggtgatg cagccgatct cggtcttcgc
     5881 gccgtcggag atgacgagca gctcgttgga gtggaagagg cgcgggatct gcgcctggta
     5941 agtctggagc tgattgtagg cctgctcgat cgtcgcgctc tcgtccgccg tattcttcag
     6001 ttcgacgacg gcgagcggca ggccgttgac gaagatcacg atgtccggcc ggcgctcgat
     6061 gcggccgtcg acgacggtga actgattgac cgccagccac tcgttgttgg ccggctcgtc
     6121 gaagtcgatg aggacgatgc gctcgccgcg cggctcgcca tccttcagca cctcgatctt
     6181 gattccgtcg accagcagct tatggaacgc acggttggcc tgcaccagca gcggcgaacc
     6241 gatctggccg acgcgccgca ccacctcctc gcgggcctcc agcgacgcgt gcgggttcag
     6301 ccgcgtcagc gcgtcggaaa gccgtccccg cagcagcaca tcgtggtagt cgctgcgctc
     6361 cggcgcgcgc ccgtctaccg cgatgtcggg gccgtagagg atttcgtaat ccagttgccg
     6421 cagccattcg agcgcggcgt cctcgactac ggattcggtg aacccggcca tgtcatcgcc
     6481 ttgtcagggt tctcatcatc gaatgcccac cgtgaagggt tctccaaaat ataacgcgtg
     6541 agacgatcca acgccgcgtc accccgaatg atgtg
//
LOCUS       DASUBI010000623         4756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1044325, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000623 DASUBI010000000
VERSION     DASUBI010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044325"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1644
                     /locus_tag="VFU72_12040"
     CDS             244..1644
                     /locus_tag="VFU72_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEU5424266.1"
                     /translation="MTKPRTLGELRESGYTVLPVREEMRKNLIAKLRADEPIFPGIIG
                     YEETVVPQVENAILSGQDIIFLGERGQAKTRMARALANLLDAEVPVVAGSEINDNPFA
                     PISRYAVDRIAEEGDRTPVDWLPRENRYAEKLATPDTTIADLIGEVDPIKVAEGRYLA
                     DELTIHYGLIPRTNRGIFAMNELPDLAERIQVGLLNIMEERDVQIRGYRLRLPLDVYV
                     VASANPEDYTNRGRIITPLKDRYGSQIRTHYPETIDLEIAIMEQEHIAFPTDGVDVTV
                     PAFMREIVAELTQLARRSHDVSQRSGVSVRMSIANYENIVSNSVKRAIRLNERQAVPR
                     VSDLHALAASTTGKIELETMGDVQEDRVVDKLVQGAVLTTFNRYFSPRDFDDLVLGFE
                     NGLVVDASDLQPAMSYVHQVSHMEGMRDAVSTLGASGNPQAIASAVEFVLEGLHLSRR
                     LNKDRGNGRDGTRYRG"
     gene            1648..3888
                     /locus_tag="VFU72_12045"
     CDS             1648..3888
                     /locus_tag="VFU72_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5424267.1"
                     /translation="MPNPFAWDFRYSRWDGTQQIDPFDADQIMDAISDDVLADGDLTR
                     ALQRLFRWGSDKPDGQQMPGMRDLLERIKQMRQREMQRYDLGSVLDDIQKKLEEIIQT
                     ERQGIDRRLQEGRERLDQQGQPGESGQEGAEGQEGQDGQQGQQGQQSQQGQPGQQGQR
                     GQRGQRGQQGQQGMPGQPGESGEGEGEGEGQEGDYTEALQKLLERMAAKNQQALDQLP
                     SDPAGRIKDLQEYDFMDPNARQMFQELLASLQQQMLQQTFQGMQQALQNMTPEDMANM
                     RNMLRELNEMLEARMRGEEPDFERFMHKYGQYFGSNINSLDELLDHLARQMAAMQSLL
                     DSMTPEMRQQLQEAMDAVMSDPGIQEQLSQLGQNLGRMMPDNPYRQPYDVAGGEELSL
                     GEAMRLMERLREMEEIEDQLKGVRDWRDLEHVDNDKIRELLGDEVNQNLEQLRQLTKL
                     LEDAGYIQRTRRGFELTPRGVRKIGQKALKDIFQHLKRDRFGQHELERSGRGGERTDD
                     TKPYEFGDPFLLHLPKTVMNAVYRQGVGSPVALAPDDFEVFRTELVTQSSTVLMVDMS
                     RSMLYNGCFAAAKKVALALDSLIRSQFPRDNLYIIGFSYVASELKPSMLPHINWDEYN
                     YGTNMQHGFMLSRQLLGRHKGGTKQIIMITDGEPTAHFDGSQVRFSYPPTYQTLQETL
                     KEVVRCTREQITINTFMLERSPYMAGFVSEMAKINKGRAFFSTPDHLGEYILVDYVTN
                     KKRHVH"
     gene            complement(4073..>4756)
                     /locus_tag="VFU72_12050"
     CDS             complement(4073..>4756)
                     /locus_tag="VFU72_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5424268.1"
                     /translation="TDLAVVRVHLGGLIPARLGDSATLRPGQLVIAIGNPYGFDSTVS
                     AGVVSALGRSMRAMSGRLIDDVIQTDAALNPGNSGGPLVNSRGEVVGVNTAMIMGAQG
                     ISFAIAINTATFIAGRLIRDGRIQRALIGIGGQTAPIHRRIARFYHVPVETGVLVTTV
                     EPGSPAERAGIQAGDVIVALGEEPVASVDALHKLLTDQRVGVSVPLTVIRHTEQLEIA
                     VVPEAAPGR"
BASE COUNT          908 a         1607 c         1581 g          660 t
ORIGIN      
        1 agacgagaac gtcggcgcca ccgtgtccac gccggtgagc gcgatcatct cgcaggttga
       61 cggccaggag aaggcgctcg tgcccgtcgc cggagagggc cgcgcgattg acatatgcct
      121 tactggtagt acgattgtcg ccacacaacc gtctcggcca gactgagggc tcggcggacg
      181 aggtacctcg gccacgcgcg gtgacacgat ccccctacag gcatggaaaa ggagccggag
      241 cagatgacga agccgcggac gctaggcgaa ctccgcgaga gcggctatac ggttcttccg
      301 gtgcgcgagg agatgcgcaa gaacctgatc gcgaagctgc gggccgacga gccgatcttc
      361 cccggcatca tcggctacga ggaaaccgtc gtgccgcagg tggagaacgc catcctctcc
      421 gggcaggaca tcatcttcct cggcgagcgc ggccaggcca agacgcgcat ggcccgcgcc
      481 ctggccaacc tgctcgatgc cgaagtgccg gtggtggccg ggagtgagat caacgacaac
      541 cccttcgccc caatcagccg ctacgccgtc gaccgcatcg cggaggaggg cgaccggacg
      601 ccggttgatt ggctgccgcg cgagaatcgc tacgccgaga agctggccac gccggacacc
      661 accatcgccg acctgatcgg cgaggtcgat ccgatcaagg tggccgaggg ccgctacctg
      721 gccgacgagc tgaccatcca ctacggcctg atcccgcgca ccaaccgcgg catcttcgcc
      781 atgaacgagc tacccgacct ggccgagcgc atccaggtgg gcctcctgaa catcatggaa
      841 gagcgcgacg tgcagatccg cggctaccgg ctgcgcctgc cgctcgatgt ctacgtggtc
      901 gccagcgcta acccggagga ctacaccaac cgcggccgca tcatcacccc gctcaaggat
      961 cgctacggct cccagatccg cactcactac cccgagacca tcgatctgga gatcgcgatc
     1021 atggagcagg agcacatcgc cttcccgacc gacggggtcg acgtgaccgt gcccgccttc
     1081 atgcgtgaga tcgtggccga gctgacccag ctggcccgcc gcagccacga tgtcagccag
     1141 cgttcgggtg tcagcgtgcg catgtcgatc gccaactacg agaacatcgt cagtaactcg
     1201 gtcaagcgcg ccatccgcct caacgagcgc caggccgtgc cgcgcgtcag cgacctgcat
     1261 gcgctggccg cctcgaccac cggcaagatc gagctggaga cgatgggcga cgtgcaggag
     1321 gaccgcgtgg tcgacaagct ggtgcaggga gcggtgctga ccaccttcaa ccgctacttc
     1381 tcgccgcgcg acttcgacga cctggtgctc ggcttcgaga atgggctggt cgtcgacgcc
     1441 tcggacctac agccggccat gagctacgtg catcaggtct cgcatatgga ggggatgcgc
     1501 gacgcggtca gcacgctggg cgccagcggc aacccgcagg cgatcgcctc ggcggtcgag
     1561 ttcgtgctgg aggggctgca tctgagccgg cggctcaata aggatcgtgg caacggccgg
     1621 gacggtacgc gctatcgggg gtaacagatg cccaatccat tcgcctggga ttttcgctac
     1681 tcgcgctggg acggcacgca gcagatcgac cccttcgatg ccgaccagat catggacgcc
     1741 atctccgacg acgtgctggc cgacggcgac ctgacgcgcg ccctccagcg cctcttccgc
     1801 tggggcagtg acaaacccga cggccagcag atgcccggca tgcgcgatct cctggagcgc
     1861 atcaagcaga tgcgccagcg tgagatgcag cgctacgacc tcggctcggt gctggacgac
     1921 attcagaaga agctggaaga gatcatccag accgagcgcc agggaatcga ccgccgcctg
     1981 caagaggggc gcgagcgcct ggaccagcag ggccagccgg gcgagtctgg ccaggagggc
     2041 gccgaagggc aagagggcca ggacgggcag caaggccaac agggtcagca gagccaacag
     2101 ggccaaccgg gacagcaagg tcaacgtggg cagcggggcc agcgtgggca gcagggccag
     2161 caggggatgc ctgggcagcc gggcgagagc ggcgagggtg agggcgaagg cgagggccag
     2221 gagggcgact acaccgaggc gctgcagaag ctgctggagc gcatggccgc caagaaccag
     2281 caggcgctcg accagctccc ctccgacccc gccgggcgca tcaaggacct gcaagaatac
     2341 gatttcatgg accccaacgc ccgccagatg ttccaggagc tgctggcgtc cctgcagcag
     2401 cagatgctgc agcagacctt ccaggggatg cagcaggcgc tgcagaacat gacccccgaa
     2461 gacatggcca acatgcgcaa catgctgcgc gagctgaacg agatgctcga ggcgcgcatg
     2521 cgtggcgagg agcccgactt cgagcgcttc atgcacaagt acgggcagta cttcggttcg
     2581 aacatcaaca gcctcgacga gctgctcgac cacctggcgc gccagatggc cgccatgcaa
     2641 tccctgctcg actcgatgac gcccgagatg cgccagcagt tgcaagaggc gatggacgcg
     2701 gtcatgagcg accccggcat ccaggagcag ctctcgcagc tggggcagaa cctcggccgt
     2761 atgatgccgg acaaccctta ccgccagccc tacgacgtcg cggggggcga ggagctctcg
     2821 ctcggcgagg cgatgcgcct gatggagcgg ctgcgcgaga tggaggagat cgaggatcag
     2881 ctcaaggggg tacgcgactg gcgcgacctg gagcacgtcg acaacgacaa gatccgcgag
     2941 ttgctgggcg acgaggtcaa ccagaacctg gagcagttgc gccagctcac caagctgctg
     3001 gaggacgcgg gctacatcca gcgcacccgc cgcggcttcg agctgacccc gcgcggcgtg
     3061 cgcaagatcg gccagaaggc gctcaaggac atcttccagc acctcaagcg cgaccgcttc
     3121 ggccagcacg agctggagcg ctccgggcgc ggcggcgagc gcaccgacga taccaagccg
     3181 tatgagttcg gcgacccctt cctgctgcac ctgcccaaga cggtgatgaa cgccgtctac
     3241 cgccagggcg tcggcagccc ggtggcgctc gcgccggacg acttcgaggt cttccgcacc
     3301 gagctggtga cgcagtcatc caccgtgctg atggtcgaca tgtcgcgctc gatgctctac
     3361 aacggctgct tcgccgccgc caagaaggtg gcgctggcgc tcgacagcct gatccgctcg
     3421 cagttcccgc gcgacaacct ctacatcatc ggcttctcct acgtcgccag cgagctgaag
     3481 ccctcaatgc tgccgcacat caactgggac gagtacaact acggcaccaa catgcagcac
     3541 ggcttcatgc tctcgcgcca gctcctcggg cggcacaagg gcggcactaa gcagatcatc
     3601 atgatcaccg acggcgagcc gacggcgcac ttcgacggct cgcaggtgcg cttctcctac
     3661 ccgcccacgt atcagacact gcaggagacg ctgaaggagg tcgtgcgctg cacccgcgag
     3721 cagatcacga tcaacacctt catgctggag cgcagcccct acatggcggg cttcgtgagc
     3781 gagatggcca agatcaacaa gggacgggcc ttcttctcga ctcccgacca cctgggcgag
     3841 tacatcctgg tcgactacgt gaccaacaag aagcgccacg tgcactgacc gcgcgccgct
     3901 tccttcgggg cagacctctg tgtctgccct gtcgccccaa cgtcttcgcg acggggtcta
     3961 cgcaagcgtt ggcggcgccg acaggatccg gcaacaagcc aacgagggca ggcacggagg
     4021 cctgccccta ccaggaatgc gccgcgccgg accagggcat cctcaactcg gattagcggc
     4081 cgggcgctgc ctccggcacc accgcgatct cgagctgctc ggtgtggcgg atgaccgtca
     4141 gcgggaccga gacgccgacc cgctggtcgg tcaggagctt gtgcagcgcg tcgacgctcg
     4201 ccaccggctc ctcgccgagg gcgacgatca cgtcgcccgc ctggatgccg gcgcgctcgg
     4261 ccggagagcc cggctcgacc gtggtgacca gcacgcccgt ctcgaccggc acgtggtaga
     4321 agcgcgcgat gcgacggtgg atcggcgccg tctgaccgcc gatgccgatc agcgcgcgct
     4381 ggatgcggcc gtcgcggatc agccggcccg cgataaaggt cgcggtgttg atcgcgatgg
     4441 cgaagctgat cccctgcgcg cccatgatca tggcggtgtt gacgccgacc acctcgccgc
     4501 gcgagttgac cagcgggccg ccggagttgc ccgggttgag cgcggcgtcg gtctggatga
     4561 cgtcgtcgat cagacgcccg ctcatggcgc gcatcgagcg cccgagcgcg ctgaccacgc
     4621 cggcgctgac ggtcgagtcg aagccgtagg ggttgccgat ggcgatgacc aattggccgg
     4681 ggcgcagcgt ggccgagtcg cccagccgcg ccgggatcag accgcccagg tggacgcgca
     4741 cgacggccag atcggt
//
LOCUS       DASUBI010000624         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_72699, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000624 DASUBI010000000
VERSION     DASUBI010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72699"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFU72_12055"
     CDS             <1..985
                     /locus_tag="VFU72_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424269.1"
                     /translation="IERNAHLQVKLVEDLLELNRAAQGKIVLDLKVLCLDDVLGAAVE
                     ALADVAEAKQITLTLVHTGERICVAADQDRLQQVFRNILSNALKFTPNGGAVAVTLAR
                     DGDWATATVRDTGEGIAPEFIPSMFHMFRQQEEGTRRRHPGLGIGLALVKRLTEAHGG
                     TASLTSGGRNKGTEVVIRLPAVAEPSEQMHSSPREAKLDDELRGTRVLVVEDVEDARD
                     SARAMFEHLGAEVLLAADGQDALAVLQNEQVDIVFCDLRMPNMDGYEFIRALHDRDGS
                     QPPVIAISGQASSGDHRRTHEAGFAGHIDKPFVDTDLLSAVSAVRARQRAN"
     gene            complement(1298..1846)
                     /locus_tag="VFU72_12060"
                     /pseudo
     CDS             complement(1298..1846)
                     /locus_tag="VFU72_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT          444 a          748 c          749 g          288 t
ORIGIN      
        1 gatcgaacgg aacgcacacc tccaggtcaa gctggttgag gatctgctcg aactcaatcg
       61 ggccgcccag ggcaagatcg tcctcgacct gaaggtcctc tgcctcgacg acgtgctggg
      121 tgcggccgtg gaggccctgg cggacgtcgc cgaggcgaag cagattaccc tcaccctggt
      181 acacaccggt gagcggatct gcgtggcggc ggatcaggat cgcctgcagc aggtgttcag
      241 aaacatcctc agcaacgcgt tgaagttcac gcccaatggc ggagcggttg ccgtgacgct
      301 ggcacgagac ggggattggg cgaccgccac cgtacgggac accggtgaag ggattgcgcc
      361 cgagttcatt ccgtcgatgt tccacatgtt ccggcagcag gaagaaggta cgcggcggcg
      421 gcacccgggt ctgggaatcg gcctcgctct cgtcaagcgc ttgaccgaag cgcacggcgg
      481 gacggcgtca cttacgagcg gaggacggaa taagggcacc gaagtcgtca ttcgcctgcc
      541 agcggtcgct gagccttccg aacagatgca ctcgtcgcca cgggaggcaa agctcgacga
      601 cgaactcaga gggacccgcg tgctcgtggt agaggatgtc gaggatgcgc gtgattcggc
      661 gcgcgcgatg ttcgaacacc tgggagcgga ggtgctgctg gcagccgatg gacaggacgc
      721 cctcgccgtg ctccagaacg agcaggtcga catcgtcttc tgtgaccttc gtatgccgaa
      781 catggacgga tacgagttca tccgagcgct gcatgaccgc gacggctcgc agccgcccgt
      841 cattgcgata tcaggacagg cgagcagcgg cgatcaccgg cgcacgcacg aggcgggctt
      901 tgccgggcac atcgacaagc ctttcgttga caccgatctc ttgtcggccg tcagcgcagt
      961 gcgagccagg cagcgagcca actagacaat cctgattctc tacggcgccg gagcgggacc
     1021 ccagtgcctg gcgcagccgc aggatgtgag cgctcgcgtc gatccgcgaa tcgaccagcg
     1081 gccgaggggc aacgccgagc gcgatcagta cgacgaccag ggcgacgaac gtccaggcct
     1141 cgcgcgattt cagtcctagc cgaagcgtgg gatgcgccag cacgtcggga cggccgcaga
     1201 acaacgagaa gtacatcctc accaccgcca gccccatcag cgccgacgca acgacaaccg
     1261 caaagcccat gaccgggaac acgtcgacgg caccgtcgac cagcagctcc tggccgatga
     1321 agccgagcgt ccctgggaag ccggtgcagg cgagcccgac tgccaggaag ctgatcgcca
     1381 gcacgggcat gcgatcgtac ccgccgtgat agcgggtcag atccagtcgg ccgcgccgcg
     1441 cctccaggac ctggacgcat cgggcgagcc cggcaaaacc gaggcccgcc gagagccaca
     1501 ccagcaggcc gccggcaagc gcggtggcgc tcgtgcaatc gagatccgcc atcacgagcg
     1561 ccgactggct catgaagagg tagccgcacg ctcgccgcgc gctccgctgc acgagcgcga
     1621 gcgccgcgcc gtacacagcg gtcccgagcg ccagaaccgc gatggttcgc agcatctctg
     1681 gtgaggcccg cggcacgatc agcacgaccg tgatgtaggc gccgacctgc ggcgcgttga
     1741 acaggatcgc cgggccgagc cggccgtgat cgaatacctc cggcacccag gcgtggaacg
     1801 ggacaatgcc tttccgaatg agcgcggcga ggacgatcag ccacatcgcc gccgtctcga
     1861 gattggtgtg ctgcactccg gggctgacga gcagcacgac gcccgccgca agcagcagcg
     1921 tcgacgcgcc cagatacgcg ccgacgaccc ggcgctggtg gcggtgtgcc ggatccccga
     1981 gcgcagagag gaacacccac accgacgcca cccacgacag cagcaggatc accgcgctcg
     2041 ccgtgaggaa cgacgcgagc gtcagcaggg ccgcaagagc ggctcgccgc aggccgcgcc
     2101 ggtcgagcgc cgcacgcggc gtgaccgcga ccgtcagcag ccacaggcct gccgcgaacg
     2161 gcaggaggac ggccgacagc gcgtcgatcc gaaggatgtc ttctccacca ggaacccagg
     2221 tcaatgccg
//
LOCUS       DASUBI010000625         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_904443, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000625 DASUBI010000000
VERSION     DASUBI010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904443"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..848
                     /locus_tag="VFU72_12065"
     CDS             135..848
                     /locus_tag="VFU72_12065"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU5424270.1"
                     /translation="MSQTQDRAGQRNPARGGGATTAAPRLVVVDEPAAPVAEAYRSLR
                     ATVKFSSLMPPPRALLLADAGTGGQHPTVAANLAAALALAGDGTILVDANLHEPRLHE
                     LFGLANEAGVAEWLAAGDATAPLPLRESGIPGLRLLGAGHLPGQVAPSDLLSGEAGAW
                     LLERLREAADFVVLDAAPLPETADALALAARVDGVLLLVRSGTTKRVPAQRAKAALER
                     VGARILGAVLTDASGRPRG"
     gene            complement(859..1608)
                     /locus_tag="VFU72_12070"
     CDS             complement(859..1608)
                     /locus_tag="VFU72_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEU5424271.1"
                     /translation="MGFYGDLEDLPLQDILYVLSTRAKSGQLTLKTGEQEFVLMLDQG
                     RIAAVTTNDDSLRIGRLLVDQAYVTEEQMEQALALQKVAERPVRIGDVLVQLGFVSRK
                     EISQAVAAQLEASLFRVLLHPSGAFAFTHEGAIRADPLMEEISIEAMILIAVRRADEW
                     IAAQAAEERVALSNTPFDPVTLDDLGDLERRALIAVLNGATTVRAIIKATGLPAPELW
                     GAVRGLLEAGLLDSVDDEANDRPLEHARSVA"
     gene            complement(1610..2113)
                     /locus_tag="VFU72_12075"
     CDS             complement(1610..2113)
                     /locus_tag="VFU72_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424272.1"
                     /translation="MPDERGKGGQSPTIDKAVEQEYNALRDFLARLESTRRPQPSTGE
                     GPRAGVKVIGTEPEADAGVRVPEPPRRATPPAPPSRPVPEPVIYWWVTLRDIPLLPRV
                     AALRERLAGLPGTIEAQVVDLSSRDIRLGVTTTPQVTLQQLEFAVTACHDINGAEVSM
                     HIPGRAR"
BASE COUNT          338 a          860 c          862 g          337 t
ORIGIN      
        1 cgtgctgctg gccttcgcgg tcgagtatct cgacgatacc ctgcgcggcg agcgggacgt
       61 cgcgcgcgcg ctagacttgc ctgtcgtcgc cggcattccg cgacgttgat gacggacacg
      121 aaggaggagc gcaggtgagc cagacgcaag accgggccgg gcagcgcaac ccggcacgcg
      181 gaggcggcgc gacgaccgcc gcacccaggc tggtggtcgt cgacgagccg gcagcgccgg
      241 tggccgaggc ttaccgctcg ctgcgcgcca cggtcaagtt ctccagcctg atgccgccgc
      301 cgcgagcgct cctcctggcc gacgccggca ccggcgggca gcacccgacc gtggccgcca
      361 acctggccgc cgcgctggcg ctggccggcg acggcaccat cctggtcgac gccaatctgc
      421 acgagccgcg cctgcatgag ctgttcgggc tggccaacga ggctggcgtg gcagagtggc
      481 tggcggcggg cgacgccacg gcgccgctgc cgctgcgcga gagcggcatc cccggcctgc
      541 gcctcctcgg cgccggtcac ctgccggggc aggtcgcgcc gtccgatctc ctcagcggcg
      601 aggcgggagc ttggctgctg gagcggctgc gcgaggcggc cgacttcgtg gtgctcgacg
      661 ccgcgccgct gccggagaca gccgacgcgc tggcgctggc ggcgcgtgtc gatggggtgc
      721 tgctcctggt gcgctccggc acgaccaagc gcgtgccggc ccagcgcgcc aaggcggccc
      781 tcgagcgcgt cggcgcccgc atcctcggcg ccgtcctcac cgacgcctcg ggccgcccgc
      841 gcggctgagg ccgcgcgctc aggcgaccga gcgagcgtgc tcgagcggcc ggtcgttggc
      901 ctcgtcgtcg acggaatcca gcaggccggc ttccagtagg ccgcgcaccg cgccccacag
      961 ctccggcgcc ggcaggccgg ttgccttgat gatcgcgcgc acggtggtgg cgccgttgag
     1021 caccgcgatc agcgcgcgcc gctccagatc gccgagatcg tcgagagtga ccgggtcgaa
     1081 gggcgtattg gagagcgcca cgcgctcctc cgccgcctgg gccgcgatcc attcatcggc
     1141 gcgccgcacc gcgatcagga tcatcgcctc gatcgagatc tcctccatca gcggatcggc
     1201 ccggatagca ccctcgtgcg tgaaggcgaa cgcgcccgag gggtgcagca gtacccggaa
     1261 gagcgaggct tccaattggg cggcgaccgc ctggctgatc tccttgcgcg agacgaagcc
     1321 gagctgcacc agcacgtcgc cgatgcgcac cggccgctcg gcaaccttct gcagcgccag
     1381 cgcctgctcc atctgctcct cggtgacgta cgcctgatcc acgagcagtc gtccgatgcg
     1441 cagcgagtcg tcgttggtcg tgaccgccgc gatccggccc tggtcgagca tcagcacgaa
     1501 ctcctgctcg ccggtcttca gcgtcagttg ccccgacttg gcgcgcgtgg agaggacgta
     1561 gaggatatcc tgcagcggca ggtcctccag atcgccatag aaccccatgt cagcgagccc
     1621 gacccggaat gtgcatgctc acctccgctc cgttgatgtc gtggcaggcg gtcacggcaa
     1681 actcaagctg ctgcagggtg acctgcgggg tggtcgtcac tccgaggcgg atgtcgcgcg
     1741 atgacaggtc gacgacctgg gcctcgatgg tccccggcag gccggccagg cgctcgcgca
     1801 gcgcggcgac gcgcggcagc agcgggatat cgcgcagggt gacccaccag tagatgaccg
     1861 gctcgggaac cgggcgcgag ggaggcgccg gtggcgtcgc gcggcgcggc ggctccggca
     1921 ctcgaactcc cgcgtcggcc tccggctcgg tgccgatgac cttcaccccg gcacgcggcc
     1981 cctcaccggt cgacggctgc ggccgccggg tcgattccag gcgcgccagg aagtcgcgca
     2041 aggcgttgta ctcctgctcg acggccttat cgatggtggg agactgaccg cccttgccgc
     2101 gctcgtccgg caccagcgtt cctctccgga ggcggttgcc gcgccacacg ccgcggcatc
     2161 acggtccaac ggtactgatc cggacggaga tgagtcaagc atcagaagtc ccgatgcgct
     2221 aggacgaggg taccggcgac ttgccagcac cgggtgctga ggcaatactt gcgagacggg
     2281 gcaacggcgc acgtcaaatg ggggtggagc gatggcgggc tggtggcgag cgggcatcgg
     2341 ctgcctgatg gtcgcggccg ccatcatcct gctgctcgca gcgccgcgcg cccatcc
//
LOCUS       DASUBI010000626         7313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_58615, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000626 DASUBI010000000
VERSION     DASUBI010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58615"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1755)
                     /locus_tag="VFU72_12080"
     CDS             complement(25..1755)
                     /locus_tag="VFU72_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HEU5424273.1"
                     /translation="MNDDEQYRRYRVCSNCGYHDTLAAPQRIELLVDAGSFEEFNRHL
                     VSVDPLVFADRLPYRERVLEAREHTGLTEAAVTGTARIAGHEVILAVLDFRFLGGSMG
                     SVVGEKLTSAFERAAEKKLPIITIAASGGARMQEGMLSLVQMAKTAAAAKRVHDDHVL
                     YISVLTNPTTGGIFASFASLGDITLAEPHALIGFAGPRVIRETSGREEIDSHSAEFLF
                     RHGFVDKIVERMQLRDTLATILQLVDAHAALEREERPAEMPERAPINAWEIVQMARRP
                     DRPTALDYIHRTSPQFVELHGDRTYADDPAIVGGIGEIDGRGVIFIGTERGHGEETRR
                     GGQALPEGYRKAKRLMELAQRLELPVITLIDTPGAFLGEGAEERGLAIALSECLATMS
                     VIGVPTVAAIIGEGGSGGALALGVGDRVLMQERAIYSVIAPEGAAAILYRDAGRAPEV
                     AEALKLTAADLLNLGVIDAVVPEPEGGAHANPELAAGLLKVSIVDALADLVEQSPSKL
                     VRERYRKFRRMGQTNTLMRELIAAEVGEWSARLRGTLETLRDYLPFSDTQEAAVSLPP
                     NGEGEPAGGG"
     gene            complement(2026..2862)
                     /gene="otsB"
                     /locus_tag="VFU72_12085"
     CDS             complement(2026..2862)
                     /gene="otsB"
                     /locus_tag="VFU72_12085"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU5424274.1"
                     /translation="MTDRPNPVAVPELAGRLARLLAARPAGLITDIDGTISRIAPRPE
                     AAVVEPEARAALARLARQLELVAAITGRSAQDAARLVAVPGIVVVGNHGLERLDLAGL
                     SVADAAQPYLESIGAVMRELATAPPAPGVVVEHKGASGSIHYRGAPEPAAARAAILRR
                     LAPLAARHGLRVTEGRMVLELRPPVAIDKGSALRELVALFHLRSAVMLGDDVTDVDAM
                     RALDSLRAEGRLAGCSVGVLAAETPAELLAAADVVIDGVESTVALLQQLARVGGNEEE
                     RA"
     gene            2963..4234
                     /locus_tag="VFU72_12090"
     CDS             2963..4234
                     /locus_tag="VFU72_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424275.1"
                     /translation="MPRYLQQALSYPREIKLYLIYTLLVNLGIGVYVLIFNLYLERIG
                     FRPDFIGLFNAVQTVAMGVVALGIGYLINRVGTWRCIAYGSVFFLITSIGVALTGNAA
                     AILALGALNGAATAFVTVPTMPFIIEWTPAEARSSVAALCFSLTSLSMMLGSLIGGWS
                     PRLFSVLGGVAVDSTLAFRGTLIFGIALAALGLPPMWLMRAAREPRSRFLGPTAALPP
                     LTSSKRRQVRRDLVVFVAVGLCLSFGAGAVVPFYNVFLSSVGARPGEIGTIFSLASAV
                     AALVGLAAPLAARKLGTLPAILLVRISPAPFFFLLALSPTVGLAAVAMSVRTSSANMG
                     WPIDSTYIAEILPPEARANAFSFRSGAWNLGYALASFIAGEVIVSVGYVPTFVAFGVT
                     IVVSTLGFVYYFRGRTPANIAAEDRAGLAAV"
     gene            complement(4304..5368)
                     /locus_tag="VFU72_12095"
     CDS             complement(4304..5368)
                     /locus_tag="VFU72_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU5424276.1"
                     /translation="MAQGERFKLFVSSPFEPEYIERIRQVAPDRVEVVYEPDLLPPTR
                     YVADHDGVPEFARTPEQEARYRRNLSEADFLLDFPKRMPADGTTMRDLIPRARWVQTT
                     SAGVGQQVARLGLQDSDIIVTTSSGIHAGPLAEFVFLALLAHAKELDRLERDKSQRHW
                     ERFCSDELAGKTLAVVGPGKIGRQVARVGRAFDMRGVAMGRSGGPERAAELDVDQVFT
                     REQLHQMLGQADAVVISVPHTPETEGMIDAEAFAAMKPGVVFVNIGRGTVVDEEALIE
                     GLRAGRIALAGLDVFAQEPLPAGSPLWEMPNVLFNPHSASTALRENARITDILCHNLR
                     CLLDGREGEMRNVLDKQRMY"
     gene            5496..6644
                     /locus_tag="VFU72_12100"
     CDS             5496..6644
                     /locus_tag="VFU72_12100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5424277.1"
                     /translation="MEQTAEAVIIGAGIMGCSVAYHLAARGVRDILVLERDQIARGST
                     SDAAGGIRLQFSSPTNIELAQRSLDVWEHFEEQFGVDIGLRQQGYLFLLRSETEVAEF
                     QKNLRLQQSLGVPARWVTPEEIHELNPVIVLDDVLGGTFCPRDGWADPFTATMGFASA
                     ARRLGVEIRQHTPVTGIAVTGERVTGVETSGGRIAAPLVIICAGARTRGVGNMAGVEI
                     PVDPYRRMSFVTEPFPAVPSTIPMTIDFQTSLYFHPEGPGFLFGMSNPEEPSSFRRTV
                     EDEWLLRCVEELAKRAPVFEEARVKTGWAGLYEITPDDNPVLGYLDEVSGLVVAAGFS
                     GHGFMQGPAIGACMAELILDGRATTADIRPFAPARFREGRLAQEHNVV"
     gene            6647..6937
                     /locus_tag="VFU72_12105"
     CDS             6647..6937
                     /locus_tag="VFU72_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU5424278.1"
                     /translation="MVIGIAHLSIQIPEAQSLKDKRRVVRSLTQRLQSRFNVAAAEVD
                     QLDAWQSAVVGLAVVSNSAAHADQMLSTILRFVEDNLRDGFLVDIHTEILHV"
     gene            complement(6942..>7313)
                     /locus_tag="VFU72_12110"
     CDS             complement(6942..>7313)
                     /locus_tag="VFU72_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424279.1"
                     /translation="ETEERAADADSAYGLLESWYEHVESVREARRAVAEVASVPVSMK
                     EIRQMVERARGEDDRRLVRHTRATLERSYHQRLVTLVGEVPAEPVEPLPDVLGRIAEA
                     TRSLRPELRREAEVFVAQALA"
BASE COUNT         1069 a         2653 c         2338 g         1253 t
ORIGIN      
        1 cgtccacgat ggccggctgc gcccttagcc gccgcccgcg ggctcgccct ccccattggg
       61 cggcaacgag acggcagcct cctgggtgtc ggaaaacggc aggtagtcgc gtagcgtctc
      121 cagcgtgcca cgcagccgcg cgctccactc acccacctcg gccgcgatca gctcgcgcat
      181 cagcgtattg gtctgcccca tgcggcggaa cttacggtag cgttcgcgca cgagcttgct
      241 gggagattgc tcgaccagat cggccagcgc gtccacgatc gagaccttga gcaatccagc
      301 cgccagctca gggttggcgt gcgcgccgcc ctccggctcc ggcaccacgg cgtcgatcac
      361 ccccagattc aggaggtcgg ccgcggtcag cttgagcgcc tcggcgacct ccggtgcgcg
      421 tccggcgtcg cgatagagga tcgccgcggc gccctccggt gcgatcacac tgtagatggc
      481 gcgctcctgc atcagcacac ggtcgccgac ccccaatgcc agcgcacccc cgctgccgcc
      541 ctcgccgatg atcgccgcca cggtcggcac gccgatcacg ctcatcgtcg ccaggcactc
      601 gctgagcgcg atcgccaagc cacgctcctc ggctccctcg cccaggaacg cacccggcgt
      661 gtcgatcagc gtgatgaccg gcagctccag ccgctgtgcc agctccatca gtcgcttggc
      721 tttgcggtag ccttcgggca gcgcctgccc tccccggcgg gtctcttcgc catggccgcg
      781 ctccgtaccg ataaagatca cgccccggcc gtcgatctcg ccgatgccgc cgacgatggc
      841 cggatcgtcg gcgtaggtgc ggtcgccgtg cagctccacg aactgagggc tggtgcgatg
      901 gatgtagtcg agcgcggtgg gccggtcggg ccgccgggcc atctgcacga tctcccaggc
      961 attgatcggg gcgcgctcgg gcatctccgc cggtcgctcc tcgcgctcta gcgcggcatg
     1021 agcgtctacg agttggagga tggtcgccag cgtgtcgcgc aactgcattc gttcgacgat
     1081 cttgtcgacg aagccgtggc ggaagaggaa ctcggcgctg tgcgagtcga tctcctcgcg
     1141 ccccgaggtc tcacggatca cgcggggtcc ggcaaagccg atcaaggcgt gcggctcggc
     1201 cagcgtgatg tcccccaggc tggcgaagct ggcgaagatg ccgccggtgg tcgggttggt
     1261 cagcacggag atgtagagga cgtgatcgtc gtggacgcgc ttggccgcgg ctgccgtctt
     1321 ggccatctgc accagactga gcatcccctc ctgcatgcgc gccccgccgg aggccgcgat
     1381 ggtgatgatt gggagcttct tctcggccgc gcgctcgaag gcgctggtca gcttctcacc
     1441 caccaccgag cccatgctgc cacccaggaa gcggaagtcg agcacggcca gaatgacctc
     1501 gtggccggcg atgcgcgcgg taccggtcac cgctgcttca gttagcccgg tatgctcgcg
     1561 cgcctccagc acgcgctcgc ggtagggcag acggtcggcg aagaccagcg ggtcgaccga
     1621 gacgaggtgc cggttgaact cctcgaacga gccggcatcg accagcagct cgatgcgctg
     1681 cggcgcggcg agggtgtcgt ggtagccgca attcgagcac acccggtaac ggcgatactg
     1741 ctcgtcgtca ttcaaagaag cgccgcattg ccggcaggca cccagatcgt cgccatccgg
     1801 tcgctctgtc cgtaccatcg ttacgggagt ctcgctccgg ttcttgtctt tcacgcctca
     1861 tctcccgtct gctgatcccg ccgcgcggag ttgctcccat tgtgcctgag aacggttgag
     1921 tcagtagagc gacttccgtc agctccttga ctcgcggcag tcgcatgtac agcatggacg
     1981 tacacactta gccgccacga atcccaccac gcttgcggcc cgttctcagg cacgctcctc
     2041 ctcgttaccg cccacccgcg ccagctgttg cagcagcgcg accgtactct cgaccccatc
     2101 tatcacaacg tctgccgccg ccagcaattc cgcgggtgtt tcggccgcga gcacgcccac
     2161 ggagcagccg gcgagccgac cctcagcacg caagctgtcg agcgcgcgca tcgcgtcgac
     2221 atcggtgacg tcgtcgccca gcatgaccgc cgagcgcaaa tggaagagcg ctaccagctc
     2281 gcgcagggcg ctccccttgt cgattgccac tggtggacgc aattccagca ccatgcgtcc
     2341 ctcggtgacg cgcagcccgt gacgcgcggc gagcggcgcc agccggcgca gaatggcagc
     2401 ccgcgctgcc gccggctccg gcgctccgcg atagtggatc gagccactcg cccccttgtg
     2461 ctcgaccacg acaccgggcg ccggcggcgc cgtcgccagc tcacgcatca ccgcgccgat
     2521 cgactccaga tacggttgcg cggcgtccgc cacgctcaac cccgcaaggt cgagccgctc
     2581 caaaccgtga ttgccgacca ccacaatccc cggcaccgcg accaacctgg cagcgtcctg
     2641 ggcgctccgt ccggtgatcg cggcgaccag ctcgagctgc cgcgcaagtc tagccagcgc
     2701 ggccctggcc tcgggctcga ccaccgctgc ctccggccga ggcgcgatcc ggctgatcgt
     2761 cccgtcgatg tccgtgatca gcccggccgg acgcgccgcc agcagccgcg ccaatcggcc
     2821 ggccagctcc ggcacggcga ccgggttggg acgatcggtc atcggctgct cccttcgtcc
     2881 tgggcgttgt cggtatactt cctcgtgcgt tttaccgccg tgactcccga tgcacgcgca
     2941 gtgccgggag actggagaca ttgtgccgcg ctacctgcag caggcgttga gctacccacg
     3001 cgagatcaag ctgtatctca tctacaccct gctggtgaac ctcggcatcg gtgtctacgt
     3061 gctgatcttc aacctgtacc tggagcgcat cggctttcgg cccgatttca tcggcctctt
     3121 caacgccgtg cagaccgtcg ccatgggcgt ggtggcgctc ggcatcggct atctgatcaa
     3181 ccgggtcggc acctggcgct gtatcgccta tggctcggtc ttcttcctga tcacgtcgat
     3241 cggggtggcg ctgaccggca acgccgccgc catcctggca ttgggcgcgc tcaacggcgc
     3301 cgcgaccgcc ttcgtgaccg tgccgacgat gcccttcatc atcgaatgga cgccggccga
     3361 ggcgcgctcg tcggtggcgg cgctctgttt ctcgctgacg tcgctctcga tgatgctcgg
     3421 cagcctgatc ggcggctggt cgccacgcct cttcagcgtc ttgggcggcg tggcggtcga
     3481 tagcaccctg gcctttcgcg gcaccttgat cttcggcatc gctctggcgg cgctcggcct
     3541 gccgccgatg tggctgatgc gcgcggcgcg cgaaccgcgc tcgcgctttc tgggccccac
     3601 ggccgccctg ccaccgctca ccagctccaa gcgccgtcag gtgcgccgcg acctggtcgt
     3661 cttcgtggcg gttgggttgt gcctcagctt cggcgccgga gcagtggtgc cgttctacaa
     3721 cgtctttctc tcatcggtcg gcgcgcgtcc cggcgagatc ggcaccatct tctcgctcgc
     3781 cagtgcggtc gccgcgctgg tgggcctggc cgccccgctg gcggcacgca agctgggcac
     3841 cctgccggcc atcctgctgg tgcgcatctc tccggcgccc ttcttcttcc tgctggcgct
     3901 cagtccgacc gtcggtctag cggcggtggc gatgagcgtg cgcacctcct cggccaacat
     3961 gggctggccg atcgactcga cctacatcgc cgagattttg ccgcccgagg cgcgcgccaa
     4021 cgccttcagt tttcgcagcg gcgcctggaa cctcggttac gcgctggcca gcttcatcgc
     4081 cggtgaagtc atcgtcagtg tgggctacgt gcccaccttc gtcgccttcg gagtcaccat
     4141 cgtagtcagc acgctcggct tcgtctatta cttccgcggc cgcaccccag ccaacatcgc
     4201 cgccgaagac cgtgcggggc tggcggcggt gtgacgcgct cgaacgtggt ggttgcttgc
     4261 cgctccgcaa cggcgcctgg tgttgtcctt cagcctcagc cgctcaatac atccgctgct
     4321 tgtcgagcac gttgcgcatc tccccttcgc gcccgtccaa cagacagcgc aggttgtggc
     4381 acaggatgtc ggtgatcctg gcattctcgc gcaaggcggt gctggccgag tgcgggttga
     4441 agaggacgtt cggcatctcc cagagcggtg agccggccgg cagtggctcc tgcgcgaaga
     4501 catccaaccc ggctaaggcg atccggccag cgcgcagccc ctcgatcagc gcctcctcat
     4561 cgactacggt gccgcggccg atgttgacga agaccacccc cggcttcatg gcggcgaacg
     4621 cctcggcgtc gatcatcccc tcggtctccg gcgtgtgcgg cacgctgatc accaccgcgt
     4681 cggcctgccc cagcatctga tgcaactgtt cgcgcgtgaa gacctggtct acgtccagct
     4741 ctgccgcccg ctccggaccg ccgctgcgcc ccatggccac cccgcgcatg tcgaaggcgc
     4801 ggccgacgcg cgccacctgg cgcccgatct tgcccggccc cacgaccgcc agcgtcttcc
     4861 cggccaactc gtcggagcag aagcgctccc agtgccgctg cgacttgtca cgctccagcc
     4921 ggtccagctc cttggcgtgg gccagcagcg ccaggaagac gaactcggcc agcggcccgg
     4981 cgtgaatgcc gctggaggtg gtcacgatga tgtcggagtc ctgcaggccg agccgcgcca
     5041 cctgctgccc tactcccgcg ctggtcgtct gcacccagcg ggcacgcggg atcagatcac
     5101 gcatggtcgt gccgtcggcc ggcatacgct tcgggaaatc gagcaggaaa tcggcctcgc
     5161 tgaggtttcg ccgatagcgc gcctcctgct ccggcgtacg cgcgaactcg ggtacgccgt
     5221 cgtggtcggc gacgtagcgc gtcggcggca gcaggtccgg ctcgtagacg acctcgaccc
     5281 ggtccggcgc cacctgccgg atgcgctcga tgtattccgg ctcgaagggc gaactgacaa
     5341 agagcttgaa gcgttccccc tgcgccatgc ctctctcctc catcatgcgg gtcaaccggg
     5401 ggcagcttac cacggagggg agcccgagaa tccacatgct acacttggct gaggtggctc
     5461 cggggcgagc tgccgtccct ggagaggtga agcgggtgga gcaaacggcc gaggccgtca
     5521 tcatcggcgc cgggatcatg ggctgcagcg tcgcctatca cctggccgcg cgcggcgtgc
     5581 gcgacatcct ggtgctcgag cgcgatcaga tcgcgcgtgg ctcgacctcc gacgccgccg
     5641 gcggcatccg cctccagttc tcttctccga ccaacatcga attggctcag cgcagcctcg
     5701 acgtttggga gcatttcgaa gagcagttcg gggtcgacat cggcctgcgc cagcagggct
     5761 acctcttcct gctgcgctcc gaaaccgagg tggccgagtt ccagaagaat ctgcgactcc
     5821 aacaatcgct cggtgtaccc gcccgctggg tcacgccgga ggagatccac gagctgaatc
     5881 cggtcatcgt gctcgacgac gtgctcgggg gcacgttctg cccgcgcgac ggctgggccg
     5941 atcccttcac cgccaccatg ggcttcgcca gcgccgctcg ccggctcggc gtcgagatcc
     6001 gccagcatac cccggtgacc gggatcgccg tgaccggcga acgcgtcacc ggcgtcgaga
     6061 catcgggcgg ccggatcgcc gcgccgctgg ttatcatctg tgcgggggcg cgcacccgcg
     6121 gggtcggtaa catggccggg gtcgagattc cggtcgaccc ataccggcgc atgagcttcg
     6181 tcaccgagcc gtttccggcg gtcccctcca ccattccgat gacgatagac tttcagacct
     6241 cgctctactt ccacccggag ggaccgggct tcctcttcgg catgtcgaac ccggaagagc
     6301 cgagcagctt ccgccgcacg gtggaagacg agtggctatt gcgctgcgtc gaggaactgg
     6361 cgaagcgtgc gccggtcttc gaggaggcgc gggtcaagac cggctgggct gggctctacg
     6421 agatcacacc ggacgacaac ccggtgctgg gctatctgga cgaggtgtcc ggcctggtcg
     6481 tcgcggccgg cttcagcggc cacggcttca tgcaggggcc ggcgatcggc gcctgcatgg
     6541 ccgagctgat cctcgacggc cgggctacca ccgccgacat caggccgttc gctccggctc
     6601 gctttcgaga gggccgcctg gcccaggaac acaacgtggt ctgacgatgg taatcggcat
     6661 cgcgcacctc agcatccaaa tccccgaggc gcagtcgctc aaggacaagc ggcgggtggt
     6721 gagatcgctc acccagcggc tccagagccg cttcaacgtc gccgcggccg aggtcgatca
     6781 gctcgatgcc tggcagagcg cggtcgtcgg gctggcggtc gtctccaatt cggcggccca
     6841 cgccgaccag atgctgagca cgatcctgcg cttcgtcgag gacaacctcc gcgacggttt
     6901 tctggtggat atccacaccg agatcctgca cgtctgagcg ctcacgccag cgcctgggcc
     6961 acgaatactt ccgcctcgcg gcgcaactcc gggcgcagcg agcgggtcgc ctccgcgatc
     7021 cgcccgagca cgtcgggcag cggctcgact ggctcggccg ggacctcacc gaccagcgtg
     7081 accaggcgct gatgatagct gcgctccagc gtagcgcggg tgtggcgcac cagccgccgg
     7141 tcgtcctccc cacgcgcccg ctcgaccatc tgccggatct ccttcatcga gaccgggacg
     7201 ctggccacct cggccacggc ccgccgcgcc tcgcgcacgg attccacgtg ctcgtaccag
     7261 gactcaagca agccgtacgc gctgtcggcg tccgcggcgc gctcttccgt ctc
//
LOCUS       DASUBI010000627         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1258821, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000627 DASUBI010000000
VERSION     DASUBI010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258821"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            754..1191
                     /locus_tag="VFU72_12115"
     CDS             754..1191
                     /locus_tag="VFU72_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5424280.1"
                     /translation="MAGQTGTWVTPYCWQPFDELLRAADIVSLHVPATPATYHLVDAR
                     ALALMKPAAILINTARGSVVDEAALVAALAADRLYGAGLDVAEHEPHLAPQLLGHPRV
                     VITPHLGGGTVESRHAARAHAVANVAAVLQGKPPHQPLNHPHR"
     gene            complement(1272..>2010)
                     /locus_tag="VFU72_12120"
     CDS             complement(1272..>2010)
                     /locus_tag="VFU72_12120"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU5424281.1"
                     /translation="ARIALPERGDYLCADEAQLRRIVARYLGLVSQVDDAIGRVLAEL
                     AARGELARTIVVYTSDHGDYAGQYGRYEKKGGLSFSAICRIPFLACYPAGLPQGVVTD
                     DLIEAVDLFPTLCVLAGLPVPNTVQGRPIAALTGQGVPRDSALTENRWRKAVQTSRYR
                     YVANLPAAGERDELYDCQRDPLELHNVIDEPAYVPVRAELQRLLLDRIVHAAHPTTVM
                     GGGWHDHRYDLDGRADLSQQRRVTPYD"
BASE COUNT          343 a          696 c          653 g          318 t
ORIGIN      
        1 gaacgcgtcc tacccgctcc caccccgaca gccacgccct agttggaggt cacgaggagg
       61 caaccacgca gtatggatac caatgggcgg ggcgctacga acgccgtgga ccacgcttcc
      121 gcgccccagc acgccacacg acaccgtgga cggcgtgcag acggactctt aatcagcggg
      181 ttgtaggttc gagtcctacg cggctcaccc acgaattcct agccatagca aggaaaacca
      241 aagggcgggg gtactgctac ccccgccctt tggcgtcctg gtgagtagtt tggtgagtag
      301 taggtcgcgc acacggtgat cgtacctccg tcgctcatgc atagggtgcg ctttcggtgt
      361 gtcctcccgg ttagccgagc catggcgcac cggtcccgag acttccacgc gatcaagggt
      421 cgtcacaccg gcactgccga tgtcgtcagg tggcggctct catacacggg gttgctgtgc
      481 cgctgcggcg agcaccctac ccgctacagc gaggcagacg ctgaagacaa ggccgccgat
      541 gggcgcgatg gcgagcgcgc cgacgagggc agtggtcgca gtgaccccgc cgaccgcgcc
      601 gccgtagagg agcgctcttc agcctgccgc gtcagcaggg acggcccaca cctcgcctac
      661 atgttagggt gggatgggcg ggagctgact caacacctcg ctgtgcggcg gcacctagct
      721 gtgagctgtc gccccgccct ttctcggtgc ccggtggcag gccagacggg aacgtgggtc
      781 accccctatt gctggcagcc cttcgacgag ctcctccgcg ccgccgacat cgtctcgctc
      841 catgtcccgg ccacgcccgc gacgtatcac ctggtggatg cgcgggcgct cgcactcatg
      901 aaaccggcgg cgatcctgat caacaccgcg cgcggcagcg tggtggacga agccgcgctg
      961 gtcgccgcgc tggcggcgga ccggctctat ggcgcaggcc tcgacgtcgc cgagcatgaa
     1021 ccgcaccttg cgccacaact ccttgggcat ccacgcgtgg tcatcacgcc tcatctcggc
     1081 ggtggcacgg tcgagagccg tcacgcggcc cgcgcgcatg ccgtcgcgaa tgtcgccgcc
     1141 gtacttcaag gcaagccacc gcaccagcca ctcaatcacc ctcaccgctg atcgcggcgg
     1201 tgcgtgacgc agggcggcac gccgcacatg ccgctagcgt gcggtgggca ggggcttccg
     1261 ctggcgtcaa ctcagtcata tggcgtgacc cgccgctgct ggctgaggtc ggcgcggcca
     1321 tcgagatcgt agcgatgatc gtgccatccg ccgcccatca cggtcgtcgg atgcgccgca
     1381 tgaacaatgc ggtcgagcag caagcgttgc agctcggcac gcaccggcac atacgccggc
     1441 tcatcaatta cgttatggag ttccagcgga tcgcgctggc aatcgtagag ttcgtcgcgc
     1501 tcgcccgcgg cgggcagatt cgccacgtag cgatagcgcg aggtctgcac cgccttgcgc
     1561 cagcgatttt cggtcagcgc cgagtcacgc ggcacaccct gccccgtaag cgcggcgatc
     1621 gggcgaccct ggacggtgtt tggcaccggc aaccccgcca gtacacagag ggtggggaag
     1681 aggtccacag cttcgatcag gtcgtccgtg acgacgcctt gcggcagacc agctgggtaa
     1741 caggcgagga agggaatccg gcaaatcgcc gagaacgaga gaccgccctt cttttcgtag
     1801 cggccatact ggcccgcgta gtcgccgtgg tcgctggtgt agaccacaat ggtccgcgcc
     1861 agttcacctc gcgccgccag ttcggccagc acccggccga tcgcgtcgtc cacctgcgac
     1921 accaggccca gatagcgcgc gacgatccgc cggagctgcg cttcgtcggc acacaggtag
     1981 tcaccgcgct cgggcagggc gatgcgcgcg
//
LOCUS       DASUBI010000628         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_760071, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000628 DASUBI010000000
VERSION     DASUBI010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760071"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1437)
                     /locus_tag="VFU72_12125"
     CDS             complement(166..1437)
                     /locus_tag="VFU72_12125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424282.1"
                     /translation="MSRWRRTLYTIFATQVLTLIGFSCVFPFFPLYIQTLGIAGPAVI
                     LWSGVITFSGSFTLAVASPIWGALADRYGRKPMLIRAMTGGAVVTALLIVAPNIWVVL
                     ILRVLQGTLTGTVAPARALVAAVTPREELGFSMGLMEASIFLGSAVGPFAGGFLNDRL
                     GFHGTFAVGSSLLAISAILVLFLIDEQFTPAVGAGHHSLNPVTNLRQTAVMPGLAILA
                     LVLFGANFANSVPSPVLPLLIPELHGVPVIGGVPQVGTAVGLVLAVAGLCATAAAWRT
                     RLLTNRFGYRRTLIGALIVAGAFTVPAAIVGTVWQMLVVRAAVGLCIGAALPTVSALV
                     SLMTPRGQQGSAYGLLASAELIGFALGPLVGGVIGASLGLRATFLVPAAVLILVAMLV
                     LTRVHIPPTAEATSVPHGEEPLAEEEITPAT"
     gene            complement(1554..>2755)
                     /locus_tag="VFU72_12130"
     CDS             complement(1554..>2755)
                     /locus_tag="VFU72_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU5424283.1"
                     /translation="PQCSPSRAALFTGRYPHSNGVLGLTHANFAWDLHPGERHLGQVL
                     QGAGYATALFGVQHESLRGSDAEVAARCGIDDIALGGHGAEVSDRALGWLAEAGRADR
                     PFFLELGYQEPHRLRATTHDEPDYYGFLGDYLAPDDARGVTVPPYLRDTPLARLELAE
                     LQGAVRYVDTQIGRVLAGLRDFGLDEHTLVVMTTDHGVALPRAKCSLYDPGLETALIL
                     RLPARGWRGGRTVDALIANVDIFPTVLDVVGLPAPENVQGHSLLALLDGAAGATPRDH
                     LFAEMTYHDYYDPRRCIRTEQHKLIVNFSAAPGFMDPSQSWRPRADTVVPAYPALDYH
                     PLVELYDLAADPLEWHNLAGDPAHATVRADLLHRLGRWMRETGDPLLQGAVTSPLHLR
                     AVAALAD"
BASE COUNT          491 a          912 c          979 g          373 t
ORIGIN      
        1 gccacaaagt cccccaatgg ggactgggtt tgttgcgagg ccgaggctag ctgctggcac
       61 agtggtagac cagccccagt cccgcaggga cttcgtggcg gcggagccgc ccttcgcgcc
      121 gaattcattc ggcgcaaccc cacagcgcca ccgcgcttgt ggcagctatg tggcaggcgt
      181 gatctcttcc tcagccagcg gttcctcgcc atgcggcacg ctggttgcct ccgccgttgg
      241 cggaatatgc accctggtca gcaccagcat tgccacgagg atcagcactg cggccggtac
      301 cagaaaggtc gcgcgcaggc cgagcgaggc tccgataacc ccgccgacca gtgggccgag
      361 cgcgaagccg atcagttcgg ccgacgccag cagaccgtag gccgatcctt gttgcccgcg
      421 cggggtcatc aggctgacga gcgcgctgac ggtcggcagc gccgcgccga tacagagacc
      481 cactgccgcg cggacgacca gcatctgcca gaccgtgccg acaatcgccg ccggcacggt
      541 gaaggcgccg gccacgatca aggcgccgat cagggtgcga cgatagccga agcggttggt
      601 gagcaggcgt gtgcgccagg cggcggcggt cgcgcagagg cccgcgaccg ccagcaccag
      661 gccgaccgcc gtgccgacct gcggcacgcc gccgatgacc ggcacaccgt gcagttcggg
      721 aatgagcagc ggcagcaccg gcgacggcac cgagttggcg aagttcgcgc cgaacagcac
      781 cagcgccagg atcgccagcc ccggcatcac ggccgtctgg cgcaggttgg taacggggtt
      841 cagcgagtga tgccctgcgc cgacggccgg cgtgaactgc tcgtcaatca ggaagagcac
      901 cagaatggcg gagatcgcca gcagcgacga gccaacggcg aaggtgccat gaaaccccag
      961 ccggtcattg agaaagcccc cggcgaacgg gccgaccgcg ctgccgagga agatggatgc
     1021 ctccatcaac cccatgctga aaccgagctc ttcgcgcggt gtgacggccg cgacgagcgc
     1081 gcgggccggc gcgactgtgc cggtgagcgt gccctgaagc acgcgcaata tcagcacgac
     1141 ccagatgttc ggcgcgacga tcagcagggc ggtcaccacc gcgccgccgg tcatggcgcg
     1201 gatgagcatc ggtttgcgcc cgtagcgatc ggccagcgcg ccccagatcg gcgaggcgac
     1261 agcaagtgtg aaggagccgc tgaaggtgat gacgcccgac cagaggatga cggcggggcc
     1321 ggcgatgccg agtgtctgaa tatagagtgg gaaaaacgga aagacgcagc taaacccgat
     1381 cagggtgagc acctgcgtcg cgaagatcgt gtagagcgtg cggcgccagc ggctcatgtc
     1441 ccctcagtcg catggtcgtc agtcgagtca cgttcgtcgg ccgcgcgcga acgatgccgg
     1501 gcaacgccgc gagcagtgta gcaagccagg tgggatcgtg cccgtggcgg gcgtcagtcg
     1561 gccagcgcag cgaccgcgcg caagtgtagt ggtgacgtca ccgcgccctg taagagcgga
     1621 tcgccagtct cgcgcatcca gcggccgagc cggtgcagca gatcggcgcg cacggtggcg
     1681 tgtgccgggt cgccggcgag gttatgccac tccagcgggt cggccgcgag gtcgtacagt
     1741 tcgaccagcg ggtgatagtc gagcgccggg tacgcgggca cgaccgtatc ggcgcgtggc
     1801 cgccacgact gcgacgggtc catgaacccc ggcgcggcgc tgaaattgac gatgagctta
     1861 tgttgctcgg tgcggatgca gcgccggggg tcgtaatagt cgtgataggt catctcggcg
     1921 aaaaggtggt cgcgtggcgt agcgccagcc gcgccatcga gcagcgccag caggctatgc
     1981 ccctgtacat tttccggcgc cggcagtcca accacgtcga gcacggttgg gaagatgtcc
     2041 acgttggcga tgagggcgtc caccgtgcgg ccaccgcgcc agccgcgagc gggcagccgc
     2101 aggatcagcg ccgtctccag gccagggtcg tagagcgagc acttggcgcg cggcagcgcc
     2161 acgccgtgat cggtggtcat cacgaccagg gtgtgctcat ccaaaccgaa gtcgcgcaac
     2221 ccggccagca cgcggccgat ctgggtatct acatagcgca ccgcgccttg cagctcggcc
     2281 agttccagcc gggccagcgg cgtgtcgcgc aggtagggcg gcacggtgac gccgcgcgca
     2341 tcgtcgggcg cgaggtaatc gccgagaaaa ccgtagtagt caggttcgtc gtgcgtggtg
     2401 gcgcgcaggc ggtgcggctc ctggtacccc agttcgagga agaagggccg gtctgcgcgt
     2461 cccgcttccg ccagccagcc cagcgcgcgg tcgctgacct ccgcgccgtg cccgccgaga
     2521 gcgatgtcgt caatgccgca gcgagcggca acctccgcat cactcccgcg caacgactcg
     2581 tgctgcacgc cgaagagcgc cgtcgcgtag ccggccccct gcaacacctg gccgagatgg
     2641 cgctcgccgg gatgcaaatc ccaggcgaaa ttcgcgtgcg tcaggccgag cacgccgttg
     2701 ctgtgcggat agcgcccggt gaagagcgcg gcgcgcgagg ggctgcactg cggcg
//
LOCUS       DASUBI010000629         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_958746, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000629 DASUBI010000000
VERSION     DASUBI010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958746"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFU72_12135"
     CDS             complement(<1..444)
                     /locus_tag="VFU72_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEU5424284.1"
                     /translation="MPRNLVVHGHFYQPPRHDPATGEIPREPEAAPYHDFNEKILAEC
                     YRPNAELGNFDRLSFDLGPTLGEWLRLRHPEVLARIGAADRAAVDRTGYGNALMQSFH
                     HTILPLATTRDRGTELRWGTRWFSHVFGRQPRGIWLPETAVDTATL"
     gene            complement(793..1278)
                     /locus_tag="VFU72_12140"
     CDS             complement(793..1278)
                     /locus_tag="VFU72_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU5424285.1"
                     /translation="MDRQEQIEILLDHYEHPRNRQANEQADVIMPGGNPGCGDIVTVY
                     LTVGEDQQSIKEVSFTGEGCTISQAAASILMELIEDEGWTLEDIVNTDYHTMVDLLGK
                     EAVQMRPRCATLALGTLKAAVKKLERDRLRQEAGLDAIDEQNEELGIVTGDAALNGPG
                     D"
     gene            complement(1625..2503)
                     /locus_tag="VFU72_12145"
     CDS             complement(1625..2503)
                     /locus_tag="VFU72_12145"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5424286.1"
                     /translation="MAEPQTAGYANPKLLVSTDWVQQHLNDARVRIVESDEDPLLYDL
                     WHIPGALRIDWHTDLQTPSSRDFIDQAQFAELLSRLGVANDSTVVFYGDKNNWWAAYA
                     YWFFKYMGHQDCRIMDGGRKKWEAEQRPTTREVPAYARTNYQPGDMRTELRAYRDDVL
                     THIGYEGRHKVGNGRPLVDVRSPQEYSGELLHMPDYPQEGALRGGHIPGAVNIPWGRA
                     VNEDGTIKAAEELRQLYQPQGVVPNEEIITYCRIGERSAHTWYVLHELLGYPNVRNYD
                     GSWTEWGNIVGFPIVH"
     gene            2721..3110
                     /locus_tag="VFU72_12150"
     CDS             2721..3110
                     /locus_tag="VFU72_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU5424287.1"
                     /translation="MTKQRAAILMVVRDAEKHLTAGEIFERVRRAEPTIAYGTVYRTL
                     HLLAERGVILEFPFGDQASRFDRRTDRHDHVHCAICGELVDVDVPSTLLARVVAEEQT
                     GYLISAHQTVFSGVCPDCQMAAVRTSD"
     gene            3199..3981
                     /gene="sufC"
                     /locus_tag="VFU72_12155"
     CDS             3199..3981
                     /gene="sufC"
                     /locus_tag="VFU72_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5424288.1"
                     /translation="MSTTTPLFQIENLHAGIEGNEILKGVDLTINPGEIHALMGPNGS
                     GKSTLAYVVAGHPNYEVLEGRVLYKGENVLEMAADERARLGLFLAFQYPTVIPGVSMA
                     NFLRLAVNSVREAREGKPLTPREFRRLMREKMQMLKVDETFATRYLNEGFSGGEKKRA
                     EILQLALLEPELAILDETDSGLDIDALRTVSEGVNTILKPEQSLLVITHYQRLLNYIK
                     PHYVHVMLEGRIVESGGPELALELEDKGYDWLKEQFAEKVGA"
     gene            4012..5415
                     /gene="sufB"
                     /locus_tag="VFU72_12160"
     CDS             4012..5415
                     /gene="sufB"
                     /locus_tag="VFU72_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5424289.1"
                     /translation="MVAKPELNLDQEYRYGFRDEEDYVFKSRKGLNPEIVTEISQMKG
                     EPDWMLQFRLKALEYFRKRPMPSWGADLSDMDFEDIYYYVKPTESAGKTWDEVPEYIK
                     NTFEKLGIPEAERKFLAGVGAQYESEVVYHSLRADLEKLGVIFTDMDSGVREHPEIVK
                     QYFGTIIPPNDNKFAALNSSVWSGGSFVYVPKGVKVEVPLQAYFRINAENMGQFERTL
                     IIVEEGAYVHYVEGCTAPTYSTNSLHSAVVEIIVKEGGRCRYTTIQNWSKNVYNLVTK
                     RAAAYKNATMEWVDGNLGSKVTMKYPAVWLMEPGARGEVLSVAFASDGQHQDAGGKMV
                     HVAPFTSSQIISKSISKGTGRSSYRGLVKVHKGAHDVRSNVVCDALLLDEQSRTDTYP
                     YMEIEEERVSIGHEATVSKVAEEQLFYLQSRGISEADAMSMIVSGFIEPIAKELPLEY
                     AVELNRLIQLEMEGSVG"
BASE COUNT          988 a         1752 c         1764 g          947 t
ORIGIN      
        1 taaggtcgcg gtgtcaacgg ccgtctcggg gagccagatg ccgcgcggct gccgtccgaa
       61 gacgtggctg aaccagcgcg tgccccaacg caactcggtg ccgcggtcgc gcgtggtcgc
      121 cagcggcaga atcgtatggt gaaagctctg catcagcgcg ttgccatacc cggtccggtc
      181 taccgccgcg cgatcggccg cgccgatgcg cgccagcacc tccggatgcc gcaggcgcag
      241 ccactcgccc agcgtcggcc ccaggtcgaa gctcaagcgg tcgaagttgc ccagctcggc
      301 attcggacgg tagcactcgg cgaggatctt ctcgttgaag tcgtggtagg gcgccgcctc
      361 cggctcgcga ggtatctcgc cggtcgctgg atcatgacgc ggcggctgat agaagtgtcc
      421 atgcaccacc agattgcgcg gcatacgttg gtataacctt cacactcagc agctacggcc
      481 gcgagccacc agaatcggcc ggcgaagggc gacaacggcc ccgacggcga cacgcgccgt
      541 cctccgcacg tcgtatcgac cctcccgcgg catctattat agaacgtacg gcgatagacg
      601 tggcatcacc cgcaagtccc gaaggcgtct cgcgcgtgac gcggtgtcac tgacccaagt
      661 aaagtggcgg gcggttgcgt gcgggtttgc gggtggttga cgaattcgag tggcgttggc
      721 tggtcagcgc gcggtgccgc gcgaccaggc ggcgcggacc acccgcatcc ggcaaaccgc
      781 gccgattcac gcttagtcgc ccggcccatt cagtgcggcg tcaccggtca cgatgccgag
      841 ctcctcattc tgctcgtcga tcgcatccag accagcttcc tggcgcaggc ggtcgcgctc
      901 gagcttcttg accgcggcct tgagcgtgcc gagcgccagc gtcgcgcagc gcggccgcat
      961 ctgcaccgcc tccttgccca gcaggtcgac catcgtgtgg tagtcggtgt tgacgatgtc
     1021 ctccaacgtc caaccctcgt cctcgatgag ctccatcagg atcgaggcgg ccgcctggct
     1081 gatcgtgcag ccctcgccgg tgaaggagac ctccttgatc gactgctgat cttcgcccac
     1141 ggtgagatag accgtgacga tatcgccgca gccggggttg ccgccgggca tgatcacgtc
     1201 ggcctgctcg ttcgcttggc ggttgcgcgg gtgctcgtag tgatcgagca ggatctcgat
     1261 ctgttcctgg cggtccattt tctctcctcg ttgctccggc gcggacatgg tgtgcgaacc
     1321 gcaatgtggg agccaccgct gcgcccggtc gtggccgcgg cgtatccgtt tcagggtacg
     1381 ctacggatag cgccgttacc actcaatgcg acatacgacg aacgtgccgc gcttggacgc
     1441 acggtccagc gcatccgccg gagactggtt cgcgaaacga agatcgacga ttgccggcgg
     1501 gacgaggacg ttccaggctt cgccaccgga cgcgaagccg ttgccagcaa agggggaatc
     1561 gccggacctt gcgggtccgg cgaacgggca gtgccgcccg ttcgcttgcc tccgacagca
     1621 gccactagtg tacgatcggg aagccgacga tgttgcccca ctcagtccac gagccgtcgt
     1681 agttccgtac gttcgggtag cccagcagct cgtggagtac gtaccaggtg tgagccgagc
     1741 gttcgccgat gcggcagtag gtgatgatct cctcgttcgg cacgaccccc tgtggctggt
     1801 agagctggcg cagctcctcg gccgccttga tcgtgccatc ctcattcacc gcccgccccc
     1861 aggggatatt gaccgcgccg gggatatggc cgccgcgtag cgcgccctct tgcgggtaat
     1921 ccggcatgtg cagcagctcg ccgctgtact cttgtggaga gcgcacgtcg acgagcgggc
     1981 ggccgttgcc caccttgtgt cgcccctcgt agccgatgtg ggtcagcacg tcgtcgcggt
     2041 aggcacgcag ctcggtgcgc atatcaccgg gctgatagtt ggtacgggca tacgccggca
     2101 cctcacgagt cgtgggccgc tgctccgcct cccatttttt gcgcccgcca tccatgattc
     2161 ggcagtcctg gtgccccatg tacttgaaga accagtaggc gtacgcggcc caccaattgt
     2221 tcttgtcgcc gtagaagacg acagtggaat cattagcgac cccgagacgg ctcagaagct
     2281 ccgcgaactg cgcctgatcg atgaagtcgc gcgagctggg cgtttgcagg tcggtgtgcc
     2341 aatcgatcct gagcgcgccg gggatgtgcc agaggtcgta gagcaacgga tcctcgtcgc
     2401 tctcgacgat gcgcacccgc gcatcgttca ggtgctgctg cacccagtct gtggagacca
     2461 gcagtttcgg gttggcgtag ccggctgtct gtggctctgc catgcgagct tcctcccttg
     2521 cctgtgaccg tgtcgcgaac ctcagcgtga tcgcgcgtcg caccacgata aacgggcgcg
     2581 tcaccgggca ctattccagc ggatcgcggc tgttgacgaa acccgaccca cgcggtaagg
     2641 tatagcatgg agagactgga gaatcattct caggtaggat ccgggggcag tagccggtga
     2701 cgacacccca gggaattcgg atgaccaagc agcgcgccgc gatcctgatg gtggtgcgcg
     2761 acgccgagaa gcacctgacc gcgggcgaga tcttcgaacg agtccgccgg gcggaaccga
     2821 cgattgccta cggcaccgtc taccgcacgc tgcatttgtt ggccgagcgc ggtgtgatcc
     2881 tggagtttcc ctttggcgat caggccagcc gcttcgatcg tcgcaccgac cggcacgacc
     2941 acgtccactg cgccatctgc ggagaactgg tggatgtcga tgtgccgagc acactgctgg
     3001 ctcgtgtcgt ggccgaggag cagaccggct acctgatcag cgcgcaccag actgtcttct
     3061 ccggtgtctg ccccgactgc cagatggcgg cggtccggac atccgactga gtggtcgcag
     3121 aggttgtgag ccgtggccca tcgcattcgc ggggcagcag gcctgccgcg tgagagagtg
     3181 gaggaatcgg acgaaccgat gagtacgaca accccgttgt tccagatcga gaacctgcac
     3241 gccggcatcg aggggaatga gatcctcaag ggtgtcgacc tgacgatcaa cccgggtgag
     3301 atccacgccc tgatggggcc gaacggctcg ggcaagagta cgctggccta cgtggtggcc
     3361 gggcacccga actacgaggt gctcgaaggc cgcgtcctct acaagggcga gaacgtgctg
     3421 gagatggccg ccgacgagcg tgcccgcctg gggctgttcc tggccttcca ataccccacc
     3481 gtcatccccg gcgtgagcat ggcgaatttc ctgcgcctgg cggtcaactc cgtacgcgag
     3541 gcgcgcgagg ggaagccgct gaccccgcgt gagttccgcc gcctgatgcg cgagaagatg
     3601 cagatgctca aggtggacga aaccttcgcc acccgctacc tcaacgaggg cttctccggc
     3661 ggcgagaaga agcgagccga gatcctgcag ctcgcgctgc tggagccgga gctggcgatc
     3721 ctggacgaga ccgactccgg gctggacatc gacgcgctgc gcactgtctc cgaaggcgtc
     3781 aacactatcc tcaagccgga gcagtcgctg ctggtcatca cccactacca gcggctgctg
     3841 aactacatca agccgcacta cgtccacgtc atgctggagg gacgcatcgt cgagtcgggc
     3901 ggccccgagc tggcgctgga gctggaagac aagggctacg actggctcaa agagcagttc
     3961 gcggagaagg tcggcgccta accggcggaa gagccagaac aggagcgaag tatggtcgcc
     4021 aagccagaac tcaatctcga tcaggagtat cgctacgggt tccgcgacga ggaggactac
     4081 gtcttcaagt cgcgcaaggg cctcaacccc gagatcgtca ccgagatctc gcagatgaag
     4141 ggtgagccgg actggatgct ccagttccgc ctcaaggcgc tcgagtactt tcgcaagcgc
     4201 ccgatgccct cctggggcgc cgacctgagc gacatggact tcgaggatat ctattactac
     4261 gtcaagccga cggagagcgc gggtaagacc tgggacgagg tgcccgagta catcaagaac
     4321 accttcgaga agctcggcat ccccgaggcg gagcggaagt tcctggccgg cgtcggcgcg
     4381 caatacgagt cggaggtcgt ctaccactcg ctgcgggccg acctggagaa gctgggcgtc
     4441 atcttcaccg acatggactc gggcgtgcgc gagcatcccg agatcgtcaa gcagtacttc
     4501 ggcacgatca tcccgcctaa cgataacaag ttcgccgcgc tgaattcgtc ggtttggtcg
     4561 ggcggctcgt tcgtctacgt gccgaagggc gtcaaggtcg aggtgccgct gcaagcctac
     4621 ttccgcatca acgcggagaa tatgggccag ttcgagcgca cgctgatcat cgtcgaagag
     4681 ggcgcctacg tccactacgt cgagggctgt accgcgccga cctactcgac caactcgctg
     4741 cacagcgcgg tggtcgagat catcgtcaag gagggcggcc gctgccgcta cacgacgatc
     4801 cagaactggt cgaagaacgt ctacaacctg gtaaccaagc gcgccgcggc ctacaagaac
     4861 gcgaccatgg agtgggtcga cggcaacctc ggctccaagg tgacgatgaa gtacccggcg
     4921 gtctggctga tggagccggg cgcccggggc gaggtcctct ctgtcgcctt tgcctccgat
     4981 gggcagcacc aggacgccgg cggcaagatg gtgcacgtcg cgccgttcac ctcctcacag
     5041 atcatctcga agtcgatctc caaggggacc ggccgctcaa gctaccgcgg gctggtgaag
     5101 gtgcacaagg gcgcccacga cgtgcgctcg aacgtcgtct gcgacgcgct cttgctcgac
     5161 gagcagagcc gcaccgacac ctatccctac atggagattg aagaggagcg tgtctcgatc
     5221 gggcacgagg cgaccgtcag caaggtcgcc gaggagcagc tcttctacct acagagccgg
     5281 ggtatctccg aggccgacgc gatgagcatg atcgtcagcg gcttcattga gccgatcgcc
     5341 aaggaactcc cgctcgagta cgccgtcgag ctgaaccgtc tgattcagct cgagatggag
     5401 ggctcagtcg gttagcgggg agccctcatc ccccccgccc cttctcccaa g
//
LOCUS       DASUBI010000630         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_46932, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000630 DASUBI010000000
VERSION     DASUBI010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46932"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFU72_12165"
     CDS             <1..1292
                     /locus_tag="VFU72_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424290.1"
                     /translation="GGMRKRFAAAWIAPWRNYSFRWVFLTRGSVMLGLDLFLTFIAYY
                     FASVEHVANFAAVTAALAVLALVGAAASAFGIGLLSDRIGRVGLVCFASACMAAAALV
                     FVAQPGAIPLWPLGLLFGLGFGAYSSVDWALAVDVLPSPRNAGKDMGLWSMASNLPAI
                     VAPLLGGGVIAAAAALGVTAAGYRALFALAALFMLLGAAFILLVRETVGARKLPPGQR
                     LRRRIALGWRLAPARGGRPRGWLRFWPVYERFWRLFHRVQPIPGVPHDLLRVQFARYH
                     GRPITLPDGTRVRRGDLVGTLHLNNPAVSRVAAEGSALRLLPMLAGDLTALAGWLASD
                     APPGGGDVRAICGVTLLARGAARLGFTLRERPRTLYTRLDGFFMSGLLALYNPAGLDR
                     LRRGTTYGRGPVEMWMSRAELLRRYGQRQPDGERTSD"
     gene            complement(1289..>2174)
                     /locus_tag="VFU72_12170"
     CDS             complement(1289..>2174)
                     /locus_tag="VFU72_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU5424291.1"
                     /translation="FYQLAIRNVAHYSPPVFSRATGQPDEWQILLRLTGIVAGQGAQA
                     PLDALDDLIAAQLIGREVRTRGSRVAERDAGELLAALAPRRGPERLLDFLLRSGPYGD
                     AFGAVPDGLTLARLEAAPHGVDLGPLQPRIPEVLRTPSGKIELAPEPLAADVPRLGAA
                     LNRTRPPMVLIGRRQLRSNNSWMHNLRALVKGPEACTLLVHPDDAARLGLTDGAPARI
                     STAAGCVEAPVRVTDSVMPGVVSLPHGWGHGQPGTRMGIAAEHPGVNLNRLIPVAEVE
                     PLSGNAILNAVPVEIAPA"
BASE COUNT          253 a          768 c          802 g          351 t
ORIGIN      
        1 aagggggcat gcgcaagcgc ttcgcggcgg cctggatcgc cccctggcgc aattacagct
       61 tccgctgggt ctttctcacg cgtggttcgg tgatgctggg cctcgatcta ttcctgacct
      121 tcatcgctta ttacttcgcc agcgtcgagc atgtcgccaa cttcgcggcg gtgacggcgg
      181 cgctggccgt gctggcgctg gtgggcgcgg cggccagcgc tttcggcatc ggcctgctct
      241 cggatcgcat cgggcgtgtc ggcctggtct gcttcgcctc cgcctgcatg gcggcggcgg
      301 cgctggtctt cgtcgcgcag cccggcgcca tcccgctctg gccgctcggc ctgctcttcg
      361 gcctgggctt cggcgcgtat tccagcgtgg actgggcgct ggcggtggat gtgctgccct
      421 cgccgcgcaa cgccggcaaa gatatgggcc tgtggagcat ggcctcgaat ctgccggcga
      481 tcgtggctcc gctgctgggt ggcggcgtga tcgcggccgc cgcggcattg ggcgtcaccg
      541 cggccggcta ccgcgccctc ttcgcgctgg cggcgctctt catgctgctg ggcgcggcct
      601 tcatcctgct cgtgcgtgag acggtgggcg cacggaagct gccaccggga caacggctgc
      661 ggcgccgcat cgcactcggc tggcggctgg cgccggcgcg tggcggtagg ccgcgtgggt
      721 ggctgcgctt ctggccggtg tacgagcgct tctggcggct cttccaccgc gtgcagccga
      781 ttccgggcgt gccgcacgat ctcttgcgcg tccagttcgc gcgctaccat ggccggccga
      841 tcacgctgcc cgatggcacc agggtgcggc gcggcgacct cgtcggcacg ctgcatctca
      901 ataatccagc cgtctcgcgg gtcgcggcgg aaggctcggc gctgcggctg ctgccgatgc
      961 tggccggcga cctgaccgcg ctggcaggct ggctggcgag cgatgcccca cccggcggcg
     1021 gtgacgtgcg cgcgatctgc ggcgtcacgt tgctggcgcg tggcgcggcg cgcctgggct
     1081 tcacgctgcg tgagcgcccg cgcacgctct acacgcgtct cgacggcttc ttcatgtccg
     1141 gcctgctcgc gctctacaat cccgccggcc tggaccgtct gcgccgcggc accacctacg
     1201 gccgcgggcc ggtcgagatg tggatgtcgc gcgcggagct gctgcgacgc tacggccagc
     1261 gccagccaga tggagagcgg acaagcgatt aggccggcgc gatctcgacc ggcacggcgt
     1321 tgaggatcgc gttgccggag agcggctcga cctccgcgac agggatcagc cgattcaggt
     1381 tgacgccggg atgctcggcg gcgatgccca tgcgcgtgcc cggctggccg tgtccccagc
     1441 cgtgcggcag gctcaccacg cccggcatga cgctgtcggt cacgcgcacc ggcgcctcca
     1501 cgcagccggc cgcggtcgaa atgcgggcgg gcgcgccatc ggtcagccct agccgcgctg
     1561 cgtcgtcggg atggaccagt agcgtgcagg cctccggccc cttcaccagc gcgcgcagat
     1621 tgtgcatcca cgagttgttc gagcgaagct gccgccggcc gatcagcacc atcggcggtc
     1681 gggtgcggtt gagcgcggcg cccaggcgcg gcacgtcggc ggccagcggc tcgggggcca
     1741 gctcgatttt gcccgagggc gtccgcagca cctcaggaat gcgcggctga agcgggccga
     1801 ggtctacgcc gtggggcgcg gcttcgagcc gcgcgagcgt caggccgtcg ggtacggcgc
     1861 cgaaggcgtc gccataaggg ccgctgcgca gcaagaaatc cagcagccgc tccggtccgc
     1921 gccgcggcgc cagcgccgcc agcagctcgc ccgcgtcgcg ttccgccacc cgtgagccac
     1981 gcgtgcgcac ctcgcggccg atgagctgcg cggcgatcag gtcgtcgagc gcatccagcg
     2041 gggcctgcgc gccttgaccg gcgacgattc ccgtcagccg cagcagaatc tgccactcgt
     2101 ccggctggcc ggtggcacgc gagaagaccg gcggcgagta gtgcgcgacg ttgcggatgg
     2161 cgagctggta gaag
//
LOCUS       DASUBI010000631         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_216877, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000631 DASUBI010000000
VERSION     DASUBI010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216877"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFU72_12175"
     CDS             complement(<1..363)
                     /locus_tag="VFU72_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5424292.1"
                     /translation="MEVISPLVRRWVEDARDDPEGYWARAAEQLPWFRTWDHVFEADP
                     PSFRWFSGAQTNISYNCLDHHVNRGWGGHAALIYENERGERRVYTYAQLLEEVKRVAA
                     AMRGIGIGRGDRVAVYMPT"
     gene            564..1304
                     /locus_tag="VFU72_12180"
     CDS             564..1304
                     /locus_tag="VFU72_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5424293.1"
                     /translation="MRVAVISDTHGNLDALRAVLAEVDARGPWDQIVMAGDVAMGGPF
                     PAECIDLLRQRGIAAVRGNADEALVTLSRGPMPAWAGEEGQTELNAAQLATARWINGQ
                     LSDAHIDYLAALPLRIELGGGDTPRLTIAHATPWSPHPVVQPDASVETAERLLDAAGS
                     QALASGHIHLQYARPLGERLLVGVGSVGLPFDGSPDADYAEFESAPSGWRVQLRQVQY
                     DIGPVLAAAEERGLPGREGIAARLRPNG"
     gene            1395..1892
                     /gene="mreD"
                     /locus_tag="VFU72_12185"
     CDS             1395..1892
                     /gene="mreD"
                     /locus_tag="VFU72_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5424294.1"
                     /translation="MTRLGFAFVLTVAALAQATFFPALHLLSIGPDLTLVLILLWSAE
                     RGAREGLLWAFPAGILLDMLAMDPLGFNALALLPAALIGGLGRRRIVHSGVLQPMLLV
                     IAATAAHWCVAAILGALLGAGYSLVFSARLGLLTALLNVAIVPFLYLIMLVLERLGVV
                     RAAQA"
     gene            1897..>2095
                     /locus_tag="VFU72_12190"
     CDS             1897..>2095
                     /locus_tag="VFU72_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424295.1"
                     /translation="MELVRLADRHKYAVERSNDEVFLSRRMFLFKGGAALGFGALLAR
                     LGQLQLAQAREFKNQAEGNIIR"
BASE COUNT          282 a          690 c          756 g          367 t
ORIGIN      
        1 ggtgggcatg tagacggcga cgcggtcgcc gcgcccgatg ccgatgccgc gcatggcagc
       61 cgccacccgt ttgacttcct caagcagctg ggcgtaggtg tagacgcgcc gctcgccgcg
      121 ctcgttctcg tagatcagcg cggcgtggcc gccccagccg cgattgacgt ggtgatccag
      181 gcagttgtag ctgatgttgg tctgggcgcc gctgaaccag cggaagctgg gtggatcagc
      241 ctcgaagacg tggtcccagg tgcgaaacca ggggagctgc tcggcggcgc gcgcccagta
      301 gccctccgga tcgtcgcgcg cgtcctcgac ccaacggcgg acgagcgggc tgatgacctc
      361 catgacatcc tcctcgcggc cgcgccggga gccatatccg gcaccggcgc gtcttgcgcg
      421 ctgaaccatc ggccagtagg gatggtagcg cgggcggagg ggctgtcaac cgggctttcc
      481 ggtgcggcgc ctgacgcctg atcgctgtgc cgtcacccgc cggccgtgcg atgatgcctg
      541 ggtaccgcgg aagggggtcc acaatgcggg tggcggtgat ctcggatacg cacgggaatc
      601 tcgatgcgct gcgtgcggtg ctggcggaag tcgacgcgcg gggtccctgg gatcagatcg
      661 tgatggcggg cgatgtcgcc atgggcgggc cgtttccggc ggagtgcatc gacctgctgc
      721 gccagcgcgg catcgccgcc gtgcgcggca atgccgacga ggcgctggtg acgctgtctc
      781 gcgggccgat gccggcctgg gccggcgaag aggggcagac cgagttgaac gcggcgcagc
      841 tcgcgacggc tcgctggatc aacgggcagt tgtcggatgc ccacatcgac tacctcgctg
      901 ctctgccctt gcgcatcgag cttgggggag gcgacacgcc gcgtctgacc atcgctcacg
      961 ccactccctg gagcccgcat ccggtcgtcc agccagacgc atcagtcgag acggccgagc
     1021 gcctgctcga cgccgctggc tcgcaagcgc tcgcatcggg ccatattcac ctgcagtatg
     1081 cgcgtccact cggtgagcgg ctgctggtcg gggttggcag cgtggggcta cccttcgacg
     1141 gctccccgga tgccgactac gccgagttcg agagtgcacc gtctggctgg agggtgcaac
     1201 tgcgccaggt gcaatacgac atcgggccgg tgctggcggc ggccgaggag cgtggcctgc
     1261 cggggcgcga gggcatcgcc gcgcggctgc gccccaacgg ctgaagcggg tagtcgcctg
     1321 cgagtgccgg tcccctgtgc taccatttcg gctgacgatc gtccgagatt tgcatggggg
     1381 accggtcaga gacggtgaca cgcctcggat tcgcctttgt cttgaccgtc gcggctctgg
     1441 cgcaggcgac gttctttccc gcactacact tgctgagcat cggcccggac ctgacgctgg
     1501 tgctgatcct cctctggagt gccgagcgcg gcgcccgcga agggctcctc tgggccttcc
     1561 ccgctgggat cctgctcgac atgctggcca tggacccgct cggcttcaac gcgctggcgc
     1621 tcctgccggc ggcactcatc ggcgggctgg ggcggcggcg catcgtccat agcggcgtgc
     1681 tgcagccgat gctcctggtg atcgcggcca ccgctgccca ctggtgtgtg gcggcgattc
     1741 tcggcgcgct cctgggagcc ggctactcat tggtcttcag cgcgcggctc gggctgctca
     1801 ccgccttgct caacgtcgcg atcgtgccgt tcttgtacct catcatgctg gtgctggagc
     1861 gcctgggggt ggtccgtgcg gcgcaggcat aagcgcatgg agctggtccg gctggcggac
     1921 cggcacaagt acgcggtcga gcgctcgaac gacgaggtct tcctcagccg gcgtatgttc
     1981 ctcttcaaag gtggcgccgc gctcggtttc ggtgcgctgc tggcgcggct cggtcagctg
     2041 cagctggccc aggcgcggga attcaagaat caggccgaag gcaacatcat ccgtc
//
LOCUS       DASUBI010000632         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_809902, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000632 DASUBI010000000
VERSION     DASUBI010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809902"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1656
                     /locus_tag="VFU72_12195"
     CDS             379..1656
                     /locus_tag="VFU72_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424296.1"
                     /translation="MGTVSLATRSLSTGEPGAVEQTGLDIGFISDLVLKFVYFQSMAT
                     AQVIADALCLPYFNVIDRAMSQLKREELVEVAGSQGFGEMAYQYTITPKGSVRSQELL
                     ERNSYVGPAPVTLEAYREMIRSQSIRNASVSPAAVRGTFSDLVVGDEVLDALGQAVNT
                     GGSIFLFGEPGNGKTTLAERLTELLGGSVLVPHAVLVDGQIIKVLDLQNHEPLGHPTG
                     RAESDRRWVACKRPAIMVGGELTMTSLDLVYDPISKVYESPLQMKALNGLLMIDDFGR
                     QQVGPRELLNRWIVPLEKRIDFLTLHTGKKIEIPFDELIVFSTNLKPKDLVDEAFLRR
                     IQSKVRIGNPSVDQYREIFRRQCGTLGIPFEQAGLVHLLREYYVKPKRELRSCHPRDI
                     LRTLVGIARYLNVPPRLSPELVDRACQTYFVDF"
     gene            1951..>3838
                     /gene="ftsH"
                     /locus_tag="VFU72_12200"
     CDS             1951..>3838
                     /gene="ftsH"
                     /locus_tag="VFU72_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5424297.1"
                     /translation="MRKQVNRRWLALGGMMVLAAVIVLAVVFGQKSTAQTVDLTQILN
                     SIKQGQVSKVEVSADGRDAEIFFTDHSKSHITLPATQPFTELLTASQVPTSQWPPIEI
                     SRGSSISGNVGILARVGIFLVLGVAVFFLMRRFTPGGMNSSNGRRSGFDPVRPGERII
                     TFDDVAGSEEVKEEVADIVDFLRDPNRFREVGARIPRGLLLAGPPGTGKTLIARALAG
                     EARASYFSVSGSEFVELYVGVGASRVRDLFRKAKEASPAIIFIDEIDAIGRRRGRMEQ
                     STEYDQTLNQILVEMDGFEERQTVVVVAATNRIDILDPALIRPGRFDRKVQVALPDRA
                     ARRAILEVHGRGKPFAPDVTFDEVAARTTGMSGADLSNVVNEAAILAARKRETKITRQ
                     DLLEALDRTIAGPAKKTGRFSERERKVIAYHEAGHAVVAHLLPQADVVRKVTIVSRGQ
                     AGGYTMIVPEEDRGLWTRAQLTDRLAALLGGMAAEELIFGEITTGSSNDLEQTTAIAS
                     SMVQRYGMSRRFGLLSTGSGSGELQLSQRTAFAAEQEAQEIVQLAYDTALTVLKTHND
                     DLERVTQRLLEIETIEGEELNELIGTPRPFPLPIPRPRTGYHWDQPQTNEPQQRHRAL
                     RRVGK"
BASE COUNT          684 a         1270 c         1216 g          668 t
ORIGIN      
        1 tcctgctgat accccagcag gcattgcgca cggtcggcga gcgcagcttc gtcaccgtgg
       61 tgacggacgg gcatcacgag gagcgcgaga tccacgtggg cctgagcagc ggtggtcagg
      121 ttgaagtcgc ctcgggtttg agcgcgggcg accgggtcgt cctgcactag ccagtccctc
      181 ttcgtccgtt tggttgcgcc tgtctgataa cccaagcgaa ccttcctgcc ccacccagac
      241 gaactaggac tacagtgcgc gagtctgtca ccgattggtg ctctcgcgtc gtgacgcgcc
      301 gcgcgtcgag gactacgctc tgattgagga atttggatac ggggacatgg gcaaaatgcc
      361 gcgaggagga agccactaat gggcacggtc agtctggcga cgcgcagcct cagcaccggc
      421 gagccgggcg ccgtcgaaca gaccgggctc gacatcggtt tcatctccga ccttgtcctc
      481 aagttcgtct attttcagag catggccacc gcccaggtca tcgccgacgc actctgcctg
      541 ccctacttca acgtgatcga ccgcgccatg tcacagctca agcgcgagga gctggtcgag
      601 gtcgccggca gccagggatt tggcgagatg gcgtaccagt acaccatcac gcccaaaggc
      661 tccgtgcggt cccaggagct gctggagcgc aactcgtacg ttgggccggc cccggtcacg
      721 ctggaagcct accgcgagat gatccgctcg caatcgatcc gtaacgccag cgtctcgccg
      781 gccgccgtgc gtggcacctt ctctgatcta gtggtcggcg acgaggtgct cgacgcactc
      841 ggtcaggcgg tcaacaccgg cggctcgatc ttcctcttcg gcgagccggg caacggcaag
      901 acgacgctgg ccgagcggct gaccgaacta ctcggtggtt cggtgctggt accgcacgcc
      961 gtgctcgtcg atgggcagat catcaaagtg ctggatctgc aaaatcacga gcccctgggg
     1021 catccgaccg gccgcgccga gtcggaccgt cgctgggtgg cctgcaagcg accggcgatc
     1081 atggtcggcg gcgagttgac catgaccagc ctcgacctgg tctacgatcc gatctcgaag
     1141 gtctacgagt ccccgttaca gatgaaagcg ctcaacgggc tgctcatgat cgacgacttc
     1201 gggcgccagc aggtggggcc gcgcgagttg ttgaatcgct ggatcgtgcc gctggagaag
     1261 cgcatcgact ttctgacgct gcacaccggc aagaagatcg agataccctt cgacgagctc
     1321 atcgtctttt cgaccaacct caagcccaag gatttggtcg acgaggcgtt cttacgccgt
     1381 attcagagca aggtgcgcat cggcaacccc agcgtcgacc agtatcgcga gatcttccgc
     1441 cgccagtgtg gcaccctcgg catccccttc gaacaggccg ggctggttca cctgctgcgg
     1501 gagtactatg tcaaaccaaa gcgcgagctg cgctcttgcc acccacgcga catcctgcgc
     1561 actttggtgg gcatcgcccg ctacctcaac gtccctccgc ggctcagccc cgagcttgtc
     1621 gatcgcgctt gccaaacgta tttcgtcgat ttctgattcc agcgtgaaat ccgcgccttg
     1681 cgtcgcacgc cgcggtttcg tggccgttca cgtcaccttt cgctgtctaa agcgcgagaa
     1741 cgtgagttgt gttcgggttg tcgtaagggc gtcgaggcgc ggctgcgacg gattctgtca
     1801 accgaatagg accagagacc tacagcgcgc cgcgtggagg gcgtatacta gcgccacaat
     1861 agagtagctg gaggtaggga aatccatcgc ggcggatcac gtccgcgata cctttctctg
     1921 gcccgaggta ggaatgcacg cggggagaac atgcgcaaac aagtgaatcg acgctggctg
     1981 gcgctgggcg gcatgatggt cttggcagct gtcatcgtcc tcgcggtcgt ttttggacag
     2041 aagtcgacgg cgcagacggt tgacttgacg cagattctca acagcatcaa gcaaggtcag
     2101 gtgtcgaagg tcgaggtgtc ggccgacggc cgtgacgccg agattttctt caccgaccac
     2161 agcaagagcc acatcaccct gccagccact cagcccttta ccgagctgct gacggcgtcc
     2221 caggtcccca ccagccagtg gccaccgatc gagattagcc gcggcagctc catctcgggc
     2281 aacgtcggca ttctcgcccg cgtcggtatc ttcctcgtcc tgggggtggc cgtcttcttc
     2341 ctgatgcgtc gttttacccc cggcggcatg aactccagca acggccggcg cagcggcttc
     2401 gatcctgtgc gccccggcga acgcatcatc accttcgacg atgtcgccgg gtcggaggag
     2461 gtcaaagagg aggtcgccga catcgtcgac ttcctgcggg atcccaaccg cttccgcgag
     2521 gtgggcgcac ggatcccccg cggcctgctg ttggccggac cgccaggcac cggcaagacc
     2581 ttgatcgcgc gggccctggc cggcgaagcg cgcgcctcct acttctcggt cagcggctcc
     2641 gaattcgtcg aactctacgt tggcgtaggg gccagccggg tgcgcgatct cttccgcaag
     2701 gccaaagagg cctcccccgc catcatcttc attgatgaga tcgacgccat cggccggcgg
     2761 cgcggccgca tggagcagag caccgagtac gatcagacgc tgaaccagat cctggtcgag
     2821 atggacggct tcgaggaacg gcagacggtc gtcgtcgtgg ccgccaccaa ccgcatcgac
     2881 atcctcgacc cggcgctgat tcgtccgggc cgcttcgacc gcaaggtcca ggtcgcgctg
     2941 cccgaccgcg cagcgcgtcg cgcgatcttg gaggtccacg gccgcggcaa gcccttcgcc
     3001 cccgacgtga ccttcgacga ggtcgccgcg cgtaccaccg gcatgtccgg cgccgacctc
     3061 agcaacgtcg tcaacgaggc cgccattctg gccgcacgca agcgcgagac gaagattacg
     3121 cgccaggatc tgttggaggc gctcgaccgc accatcgccg gcccggccaa gaagacgggc
     3181 cgcttctccg agcgggagcg caaggttatc gcctaccacg aggcggggca cgcggtcgtg
     3241 gcgcacctcc tgccgcaggc ggacgtggtg cgcaaggtca ccatcgtctc tcgcggccag
     3301 gcgggcggct acaccatgat cgtgcccgaa gaagatcgcg ggctctggac gcgcgcgcag
     3361 ctcacggatc ggctggcggc gctgctcggc ggcatggctg ccgaggagct gatcttcggc
     3421 gagatcacca cgggctcctc gaacgacctg gagcagacga ccgcgatcgc ctcctcgatg
     3481 gtgcagcgct acggcatgag ccgtcgcttc ggactcctgt cgaccggcag cggctcgggc
     3541 gagcttcagc tctcgcagcg cactgccttc gcggccgagc aggaggcgca ggagatcgtg
     3601 cagctcgcct atgacacggc tctgacggtc ttgaagacgc acaacgacga cctggagcgc
     3661 gtcacccagc gcttgctgga aatcgagacg atcgagggcg aagagttgaa cgagttgatc
     3721 ggcacgccgc ggccgtttcc gctgccgatc ccgcggcctc gcaccggcta tcattgggac
     3781 cagccgcaga ccaacgagcc gcaacagcgc caccgtgcgc tgcgccgcgt cggcaagg
//
LOCUS       DASUBI010000633         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_916400, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000633 DASUBI010000000
VERSION     DASUBI010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916400"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..146
                     /locus_tag="VFU72_12205"
     CDS             9..146
                     /locus_tag="VFU72_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5424298.1"
                     /translation="MIDAIHYRDYPIIQTVVLMMAITVAVINLLVDLSYAWLDPRVRY
                     S"
     gene            200..1111
                     /locus_tag="VFU72_12210"
     CDS             200..1111
                     /locus_tag="VFU72_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424299.1"
                     /translation="MASTQALAETQRFDSRSKAARRSVLSKIVANPLGLLGLIVTVLF
                     ILIAIFAPFLAPHDPGDQNLSLRLLPPAWQSGGKLSYILGADALGRDLLSRVIEGSRI
                     SLLVGFSSVLIGGLVGVAVGLLSGVRGGIVDTILMRLGDVQLSFPPLILAIAIMAVFG
                     QGLLNIILVLSISGWIQYARIVRGRALSLRSADYITAARAIGVGEGRIMLRHILPNVM
                     APIIVIASFVLPQVIIAEASLSFLGIGVPPSTPTWGNMLADSRDYIASAWWLSVWPGL
                     ALMLCLLGITVLGDALRDALDPRLKNL"
     gene            1183..1872
                     /locus_tag="VFU72_12215"
     CDS             1183..1872
                     /locus_tag="VFU72_12215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU5424300.1"
                     /translation="MPLPVDVEQAIDPRRSVLLPIDMQRRHLDVGGVGYHTLPAERAK
                     LAVERGGVALRAARDAGMPVVHVGTWSMSPAPPWGPREGRNPFWAWQNGKPIPGAGFV
                     RQATKCTEGSPYAEFMPPEQPLPHEPVVVKRRYSGFYMTDLELILRSFGVDTIFIIGV
                     NTNNCCLHTAFDAQARDFRVVLLEDACGSMNGQEYHEAALKQIEAAIGWVISVTDFTT
                     LIEKKAGALVG"
     gene            2181..>2900
                     /locus_tag="VFU72_12220"
     CDS             2181..>2900
                     /locus_tag="VFU72_12220"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5424301.1"
                     /translation="MTAERRRDNQQWMLDWLVKETGRVQNFANDERTVPAEVKSYRMI
                     PRVLAKQARHFETIARAAEDHGHTDTAAQVYWRAMRVYLEAQHTIFEDDNPEKIFLHQ
                     KTLECFDQTARYSGNLVETVEIPWEGVQIQGRFHLHKDRRRAPTVLFLPGMDMTKESF
                     PNPLGNPFLKRGMNVLAIDGPGQGISNIRKIRVGADNYERAASAAIDFLAARPEVDPA
                     QIAVVGSSFGSHWGARTAALDK"
BASE COUNT          467 a          986 c          979 g          468 t
ORIGIN      
        1 ggcggctcat gatcgacgcg atccactacc gggactaccc gatcatccag acggtggtgc
       61 tgatgatggc gatcacggtg gccgtcatca acctgctggt cgatctgtcg tacgcctggc
      121 tcgatccgcg ggtgcgctac agttgagatc cggtcggagc gctggcgaat cgtcgatcgc
      181 gctggtgagg ggagagtaga tggcgtccac gcaggctctc gcggagactc agagattcga
      241 ctcgcggtcg aaggctgcgc ggcgctcggt cctctccaag atcgtcgcca atcccctcgg
      301 gctgctgggg ctgatcgtca ccgtgctctt catcctgatc gcgatcttcg cgccgttcct
      361 ggcgccccac gatccgggcg accagaactt gagcctgcgg ctcttgccgc ctgcctggca
      421 gtcgggtggc aagctgtcgt atatcctcgg cgccgacgcc ctggggcgcg acttgctcag
      481 ccgcgtgatc gagggctcgc gcatctcgct cctggtcggc ttctcgtcgg tgctcatcgg
      541 cgggctggtg ggagtcgccg tcggtctcct ctccggggtc aggggcggca tcgtcgatac
      601 catcctgatg cgcctgggcg acgtgcagct ctccttcccg ccgctgatcc tggcgatcgc
      661 gatcatggcc gtcttcggcc aggggctgct caacatcatc ctggtgctct ccatctcggg
      721 ctggatccag tacgcgcgta tcgtgcgcgg ccgggcgctc agcctgcgct cggccgacta
      781 catcacggcg gcgcgcgcga tcggggtggg cgaggggcgc atcatgctgc gccacatcct
      841 gcccaacgtc atggcgccga tcatcgtgat cgcctcgttc gtgctgccgc aggtcatcat
      901 cgcggaggcg tcgttgagct tcctgggcat cggcgtgccg ccctccaccc cgacctgggg
      961 caacatgctg gccgactcgc gcgactacat cgcctccgcc tggtggctgt cggtctggcc
     1021 ggggctggcg ctgatgctct gcctgctcgg catcaccgtg ctcggcgatg ccctgcgcga
     1081 cgcgctcgac ccgcggctca agaacctgtg agcggagcgg ctgagattat tcattcgcga
     1141 acccatggat gcaatcgccg agagggatga aagggagaga cgatgccact accggtcgac
     1201 gtcgaacaag ccattgatcc gcgccgcagc gtgctgctgc cgatcgacat gcagcgccgg
     1261 cacctggatg tcggcggggt cggctaccac accctgccgg ccgagcgcgc caagctcgcg
     1321 gtcgagcgcg gcggcgtggc gctgcgcgcg gcgcgcgacg ccggcatgcc ggtggtgcac
     1381 gtcggcacct ggtcgatgag cccggcgccg ccctgggggc cacgcgaggg ccgcaacccc
     1441 ttctgggcct ggcagaacgg caagccgatc ccgggggccg gcttcgtgcg ccaggcgacc
     1501 aagtgcaccg aggggagccc ctacgccgag ttcatgccgc ccgagcagcc gctgccgcat
     1561 gagccggtcg tggtcaagcg gcgctacagc ggcttctaca tgaccgacct cgagctgatc
     1621 ctgcgcagct tcggcgtcga caccatcttc atcatcggcg tcaacaccaa caactgctgc
     1681 ctgcacaccg ccttcgacgc ccaggcgcgc gacttccgcg tcgtgctgct ggaggacgcc
     1741 tgcggcagca tgaacgggca ggagtaccac gaggcggcgc tgaagcagat cgaggccgcc
     1801 atcggctggg ttatcagcgt gacggatttc accacgctga tcgagaaaaa ggccggcgcg
     1861 ctggtcggct aaacggctgg gacccgcgtg gtccacggta ggggcagacc tccgtgtctg
     1921 ccctgtagcc cctacgcgga tgtacgggtg aatagggtga tgtaggtggc gttgatacag
     1981 gaccggcggt agggcgggga gggcagacac aggggtctgc ccctacagga atagcccagc
     2041 accggtcacg gcaggtgaag accgctattg gcaggcaggc cgactccgcc ggctcgcgcg
     2101 ggagagccat gcgccgggtg ggcggactgg cagggcatcg accacgagat attcaggcgc
     2161 atcgcaggga ggacgagcag atgacggccg agcggcgtcg cgacaaccag cagtggatgc
     2221 tcgactggct ggtaaaagag accggacgtg tccagaactt cgccaacgac gagcgtaccg
     2281 tgcccgccga ggtcaagtca taccgcatga tcccgcgcgt gctggccaag caggcccggc
     2341 acttcgagac gatcgcccgc gcggccgagg accacggcca caccgacacc gccgcgcagg
     2401 tctactggcg ggccatgcgc gtctacctgg aggcgcagca caccatcttc gaggacgaca
     2461 acccggagaa gatcttcctc caccagaaga cgctggagtg cttcgaccag accgcgcgct
     2521 actccggcaa cctcgtcgag accgtcgaga tcccctggga gggggtccag atccaggggc
     2581 gcttccacct gcacaaggat cgccgccgcg cgccgaccgt cctcttcctg cccggcatgg
     2641 acatgaccaa ggagtccttc cccaaccccc tgggcaaccc gtttctcaag cgcggcatga
     2701 acgtcctcgc gatcgacggg cccggccagg ggatcagtaa tatccgcaag atccgcgtcg
     2761 gcgccgacaa ctacgagcgg gcggccagcg cggccatcga cttcctggcc gcgcggcccg
     2821 aggtcgatcc cgcgcagatc gccgtggtcg gctcgtcctt cggctcgcac tggggcgcgc
     2881 gcacggcggc gctcgacaag
//
LOCUS       DASUBI010000634         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1217091, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000634 DASUBI010000000
VERSION     DASUBI010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217091"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1928)
                     /locus_tag="VFU72_12225"
     CDS             complement(231..1928)
                     /locus_tag="VFU72_12225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEU5424302.1"
                     /translation="MTATRPTPEQFAEASWDDILPMYQELAARPLDAGTVEQWLADWS
                     AFEEALFEARSLASIAYTCDTRDPAKEAAHLRFAMEIMPNVEEQHVRLSNRLLDLGYQ
                     RDDLSTTLRKMRTQRELFREENVPLQRELSSLNAQYQKLTGGMTAEWEGEQLPLPRLA
                     RFLQEPDRAVRERAYRAQAAPYVSARDALAAIFDQQYALRQQVAKNAGFANYRDYVHQ
                     EKFRYDYTPADCETFHHAVETVVVPALSRRYEQRRRQLGVEVLRPWDVSVDPFSRPPL
                     QPYATIDELTGKAEAIFQRVDPVLGDYFATMRREELLDLESRTGKAPGGYCTGLPYRK
                     RPFIFMNASGVVGNVRTLLHESGHAFHNFEAGAALPLLFQRHPGSEMAEVASMSMELL
                     SAPYLEESQGGYFTPEEASRARVEHLEGILELLPWVATVDAFQQWLYTSGQGHDAAAR
                     DRAWLEIYGRFNPGIDWSDLEAERLARWYRQLHIFLHPFYYIEYGIAQLGALQVWRNA
                     LRNQAKAVEQYRQALALGGSRPLPELFAAAGARLALDEGIFREVIGLVEEQLAALEA"
     gene            complement(2008..>2771)
                     /locus_tag="VFU72_12230"
     CDS             complement(2008..>2771)
                     /locus_tag="VFU72_12230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5424303.1"
                     /translation="RGKYAIEYWLLKPADFDPSKKYPVILDIHGGPNGHFGYNFTTRP
                     QILATNGFVVAFANPRGSSSYGREFTMQVVQDWGNEDYQDLMAVMDKVLEEPYVDPAR
                     TGIHGYSYGGYMTSWIIGQTQRFQACVCGAPCFDLESFYGTSDIGTRFGERQFGSAPH
                     EGKEWYAAHSPSNFAHRATTPTLIIHGEQDFRCPIGQGEQMFVALKKAGTEVEFARYP
                     GGAHTFARTGHPEHQVDVLGRTLKWFKDHLGEPQG"
BASE COUNT          412 a          903 c         1006 g          450 t
ORIGIN      
        1 cgtcggtggc gccatgggcg acctccggga tcgaggcgtc gaaacgtgct ggtgagcgtg
       61 gggggagtat agcaccgcgc cacatcggta gggcagatcc ctgtgtctgc cctcgcttcc
      121 cgagcgccaa tcccgcatca acgccaccta catcacccta tccccccgtg cataggcgta
      181 ggggcgaccg ggcagacaca ggggtctgcc cctacggcgg ccacgtggcg tcacgcctcc
      241 agcgcggcca actgctcctc gaccagtccg atcacctcgc ggaagatgcc ctcgtccagc
      301 gccagccgcg cgccggcggc ggcgaagagc tccggcaacg ggcggctacc gccgagggcc
      361 agcgcctggc gatactgctc gaccgctttc gcctggttcc gcagcgcgtt gcgccagacc
      421 tgcagggcgc cgagctgcgc gatgccgtac tcgatgtagt agaaggggtg aaggaagatg
      481 tgcaactggc ggtaccagcg cgccagccgc tcggcctcca gatcgctcca gtcgatgccg
      541 gggttgaagc gcccgtagat ctccagccag gcgcggtcgc gggccgccgc gtcgtggccc
      601 tggccgctgg tgtagagcca ctgctggaag gcgtcgacgg tggcgaccca ggggagcagc
      661 tccagaatcc cctccaggtg ctcgacgcgc gcccggctgg cctcctcggg cgtgaagtag
      721 ccgccctggg attcctccag gtagggcgcg ctgagcagct ccatcgacat cgaggcgacc
      781 tcggccatct ccgagccggg gtggcgctgg aagaggagcg gcagcgcggc gcccgcctcg
      841 aagttgtgga acgcgtgccc cgactcgtgc agcagcgtac gcacgttgcc gacgactccg
      901 gaggcgttca tgaagatgaa cgggcgcttg cggtagggga ggccggtgca gtagccgccg
      961 ggcgccttgc cggtgcggct ctccaggtcg agcagttcct cgcgccgcat ggtggcgaag
     1021 tagtcgccca gcaccggatc gacgcgctgg aagatcgcct ccgccttgcc ggtcagctcg
     1081 tcgatcgtcg cgtagggctg gagcggcggg cggctgaagg ggtcgacact gacgtcccag
     1141 ggacggagga cctcgacgcc gagctgccgc cgccgctgct cgtagcgccg ggacagcgcc
     1201 ggcacgacca cggtctcgac cgcgtggtgg aaggtctcgc agtcggccgg cgtgtagtcg
     1261 tagcgaaact tctcctggtg cacgtagtcg cggtagttgg cgaagccggc gttcttcgcc
     1321 acctgctggc gcagcgcgta ctgctggtcg aagatggcgg cgagcgcgtc gcgcgcggac
     1381 acgtagggtg cggcttgggc gcggtaggcg cgctcgcgca ccgcccggtc cggctcctgg
     1441 aggaagcggg ccaggcgcgg caggggcagt tgctcgccct cccactcggc ggtcatgccg
     1501 ccggtcagct tctgatactg cgcgttgagg ctcgacagct cgcgctgcag cggcacgttc
     1561 tcctcacgga acaactcgcg ctgggttcgc atcttcctca gcgtggtgct caggtcgtcg
     1621 cgctggtagc cgaggtcgag caggcggttc gagagccgca cgtgctgctc ctcgacgttg
     1681 ggcatgatct ccatcgcgaa gcgcaggtgg gcggcctcct tggctgggtc gcgcgtgtcg
     1741 caggtgtagg cgatcgaggc caggctgcgc gcctcgaaga gcgcctcctc gaacgccgac
     1801 cagtcggcca gccactgctc gacggtgccg gcgtcgagcg ggcgcgcggc cagctcctgg
     1861 tacatcggca ggatatcgtc ccagctcgcc tcggcgaact gttcgggggt ggggcgggtt
     1921 gccgtcattc ctcatcctct ccaggctgca acaagagtac cccgctcccg gagctgggag
     1981 cggggtaaac ataaacatga ccgagatcta gccctgcggc tcgccgaggt ggtccttgaa
     2041 ccacttgagc gtgcggccca gcacgtcgac ctggtgctcc gggtggccgg tgcgggcgaa
     2101 ggtgtgcgcg ccgccggggt agcgggcgaa ctcgacctcg gtgccggcct tcttcagcgc
     2161 cacgaacatc tgctccccct ggccgatcgg gcagcggaag tcttgctcgc cgtggatgat
     2221 cagcgtcggg gtggtggcgc ggtgggcgaa gttcgagggc gaatgcgccg cgtaccactc
     2281 cttgccctcg tgcggggccg agccgaactg gcgctcgccg aagcgcgtgc cgatgtcgct
     2341 ggtgccgtag aacgactcca ggtcgaagca gggcgcgccg cagacacagg cctggaagcg
     2401 ctgggtctgg ccgatgatcc aggaggtcat gtagccgcca tagctgtagc cgtggatgcc
     2461 ggtgcgcgcg ggatcgacgt agggctcctc cagcaccttg tccatcaccg ccatcagatc
     2521 ctggtaatcc tcgttgcccc agtcctgcac gacctgcatg gtgaactcgc ggccatagga
     2581 ggaggagccg cgcgggttgg cgaaggcgac gacgaagcca ttggtggcca ggatctgcgg
     2641 gcgggtggtg aagttgtagc cgaagtggcc gttggggccg ccgtggatgt cgaggatgac
     2701 cgggtacttc ttggaggggt cgaagtcggc cggcttgagc agccagtact cgatggcgta
     2761 cttgccgcgc t
//
LOCUS       DASUBI010000635         6732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_590623, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000635 DASUBI010000000
VERSION     DASUBI010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590623"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFU72_12235"
     CDS             complement(<1..525)
                     /locus_tag="VFU72_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424304.1"
                     /translation="MILRHSYTRSRATAHAALRYYHLRPRGDGEPPRQFFGPAGTLTR
                     GEACRLLDQHQPAAGRYLAHRLMLSPSDTECPDDLRAMTYQAMRGLAHAHDQHLAWVA
                     VEHCNTDQPHVHILLAGGDLAGGRGEVRLGPVDHARLKAEAADYCRLEARLRRDWDRA
                     LAHAASAEERTAAER"
     gene            complement(530..967)
                     /locus_tag="VFU72_12240"
     CDS             complement(530..967)
                     /locus_tag="VFU72_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424305.1"
                     /translation="MAKTRLAIYLDTATAEALASWQLRHRESCPSLSEAGQWLLARAL
                     LADLNPGAESLIAPYLERRLTAAIGGLVREELSSLLRAQTDRLAALLVRSGKDARTSA
                     RLATAILEQLTGDHAFARRLAEEARLAAGPAYTAQGLRATGED"
     gene            complement(1202..1357)
                     /locus_tag="VFU72_12245"
     CDS             complement(1202..1357)
                     /locus_tag="VFU72_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424306.1"
                     /translation="MVRNVARLRLVVYLLIALVGLAVFGVLGCLALQLFVWGGLTHWG
                     VPLTPVP"
     gene            complement(1478..2902)
                     /locus_tag="VFU72_12250"
     CDS             complement(1478..2902)
                     /locus_tag="VFU72_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424307.1"
                     /translation="MLIGGVPYDASRDQGCADCGPNGRVESAQARVYRWAHDCQQSSQ
                     PACQTADGALADSVARLYGEYQCQGRDWTTSGFTALPPDWATSRHNDPALFASPTCGG
                     VSPATAGSGASDSGGVSNESSNGSGQACVGSLCVSVSIPLPGPTQLAHAVLAALNWED
                     VLRQLLQAIYRVIVGDAFEQLGEQGTQILLLMPDLRTSGGAMGNIQRLVDALREAAVA
                     LCLVVFAFSLVQYLFGQESEPFGLFTRLGAVLFALAYYRLLLGWLLGGCNAVAGGILT
                     IGADQTTDILPNLLKGLVPVTAPLWYLVALVGLVFLMALLMVKIAGFATLLILYVAGP
                     LLLPLALHHRTAPWVGLWLEHLVKALLWPALWALELRLFGALANGLSLVDPTGHVALQ
                     ALEGSALGALTALAMLGLMVVTPWGFHTRFTVRQGAAVLSRTAMRAADSVVLVTSGGT
                     TMSARVALANSLAARRQPTEAATE"
     gene            complement(3108..4235)
                     /locus_tag="VFU72_12255"
     CDS             complement(3108..4235)
                     /locus_tag="VFU72_12255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5424308.1"
                     /translation="MTRWLALGGLGCGAVFGLPLLGLTLLAALAGAVVPALPALALGG
                     SQPGGNSGTYPGPSGAGPTIHNLGFGPSDAVRIDRLIAALAPDSPLRGYGATIVIFGQ
                     RFGIDPLLIVVWQLESQLDTVGLNVPGNGGNLIWEAMAPYAAAWGCTPGPASLGHQWG
                     TCPSVTAGLGIWFQYVAQSPVYAMAADLVAFANRYNPCSDPANAANGFACGDAYAAQI
                     LALLRTAAGPTLAPTGYVFPVVGFTGSIDLHWGSYPGAADLFAPAGTPILAMRGGHVA
                     YADYDTLGGWAVLIAGDDGLQYWYAHLASRPLVSAGQLVTAGTPLGVIGATGNADGGA
                     PHLHLGIGPTILTGSGPTGGAGSDHFDAVSLLRQVYACCRR"
     gene            complement(4242..>6732)
                     /locus_tag="VFU72_12260"
     CDS             complement(4242..>6732)
                     /locus_tag="VFU72_12260"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424309.1"
                     /translation="CTGVNLALAGDATAAQLAAQFATVLTYLPPGVRLQLVVMNRPHR
                     AAAWVPAHLAQYQAPPALGPYVAQLADAYQQLLADQPVPDLRFYAVVVVPGPPAPRGP
                     FARRARRQRRLVRDRQQQARALGELDRAVAGVQQTLAQLGVPTTPLGRQATIDLLWMC
                     LNPAWSQHATAPRAAGHEVHPPDLRSLRERLAQSRLTRRTEYLRLDWGYETTLSLRAL
                     PELTFPGWLAQLTASGVAFRFALHIVPLPKARERTALTRRLRQRHGVLAERDQQGLPP
                     DLEQRQAYQEAEQLLTAMSAGDLRTFRTAVFVTVRAGSPEALAGAAGQVVRALGDAGG
                     TSIDRCQFWQLPAWGATLPLAHNPAGLTYRTITTNLADSLPFLRHSAGTRGGPLIGFS
                     QPGHEVVPLDMFDPALPNGNLIVCGTSGSGKTLFAQTYALKHLALGGRVLVLDRSTDH
                     WADLVAAVGDAELHQVSLGSGLRLNPWQLPAGVREPGQAKVQYLLDLHQLLLGEEHDG
                     VRQLTTTETALLEGACRAVYGATPTPRERDLYAWLRDQAQAAEDTERRQRYDSLAERL
                     LPYVEDGTYAGLLDGPTTVRSAALLTVFNFKHLSDRLVPLVMLPLIEHIWQVVADPTK
                     PILLILDEGWKLLEHPASARLLAEVARTGRHHGLVSLNLSQFITDYTGPTGQAILQNA
                     AVALLLQQHPTLLPTAQTLFGLTDDERLALGRLSTVKGVKAGAYLYAESGAQRGLVDL
                     VVTPVEYWLCTSDPAEQRLRQRSIARHAGDIWAAIADLAAQTSPPRTARRTGQMPALA
                     SDGQRTCQSTDVRGARCLMRITTANGGSAPE"
BASE COUNT         1098 a         2242 c         2406 g          986 t
ORIGIN      
        1 acgctcggcc gctgtgcgct cctcggcgga ggcggcgtgt gccagcgccc ggtcccaatc
       61 cctgcgcagg cgcgcttcca gtcggcaata gtccgccgcc tccgctttga gccgggcatg
      121 gtccactggg ccgaggcgca cctcgccgcg accgcccgcc aggtcgccac cggcgaggag
      181 gatatgcacg tggggctggt cggtgttgca gtgctcgacc gcgacccagg cgaggtgctg
      241 gtcgtgcgcg tgcgcgagtc cacgcatggc ctgataggtc atcgcccgca ggtcgtcagg
      301 acattcggtg tcactgggtg agagcatcag ccggtgcgcg agatagcgcc ccgcggcggg
      361 ctggtgctgg tcaagcagcc ggcaggcttc gccacgggtg agcgtaccgg cggggccgaa
      421 gaattgtcgg ggtggttcac cgtccccgcg cgggcgcaga tggtaatagc gcagggcggc
      481 gtgggcggtg gcccggctgc gggtgtagga gtggcgcagg atcatcgcct cagtcctccc
      541 cggtcgcgcg cagaccctgc gcggtgtacg ccggaccagc ggcgagacgg gcctcctcgg
      601 cgaggcggcg cgcgaatgca tgatcgccgg tgagctgctc gaggatcgcc gttgcgaggc
      661 gggcgctcgt ccgcgcatcc ttgccgctgc ggacaagcag cgcggcgagg cggtcggtct
      721 gcgcgcggag caatgagctc agctcctcgc gcaccagacc gccgatcgct gcggtgagac
      781 ggcgctccag gtacggcgcg atcaggctct ccgcccccgg attgaggtcg gcgaggagcg
      841 cgcgggcgag caaccactgc cccgcctcgg agagcgaggg gcaggattcc cggtggcgca
      901 gttgccagct ggctagcgcc tccgctgtcg ccgtgtcgag gtagatcgcc agtcgcgtct
      961 tcgccatgct catcctcccc ggcgttagct cggtgttagt gcctcgcgcc gccgcgctac
     1021 gacgggcgaa tcgggacgcg cagcacgcgc gcacctgggc ctaattcgac cgttagtgcc
     1081 gcacatttct cagcgactgc gcaggaaacg gcgctggagg caccgccctt tatcttgcct
     1141 tcggcgccgg tgggcgaccg ccggtctccg acctggctag ccagtctatt catctgcctg
     1201 ctcatggcac gggtgtcagc ggcacgcccc agtgggtcaa tccgccccag acgaaaagct
     1261 gcagcgccag gcagcccaac acgccgaata ccgccagtcc aaccagggcg atcaggagat
     1321 agaccactag gcggagccgg gcgacgtttc gcaccatatg cccaatatca gtctcaatcg
     1381 gtgacccaga ctgctgcgta cccattgctg tctccctcct gtgtctcttg gcaccggtcc
     1441 ccagcagcat gtgttgtggc acgctccacc agcaccgtta ctccgttgcc gcctcggttg
     1501 gctggcggcg cgccgcgaga ctgttggcca gggccacccg ggcactcatc gtagtgccac
     1561 cactggtcac cagcaccaca ctatcggccg cgcgcatggc cgtgcggctg aggactgcgg
     1621 caccctggcg caccgtgaaa cgggtatgaa aaccccaggg cgtgacgacc atcaatccga
     1681 gcatcgccag ggcggtaagc gcgcctaacg cactcccttc cagcgcctgg agcgccacat
     1741 gcccggtggg atcgactagg ctgaggccgt tcgccagcgc accaaagagc cggagttcca
     1801 gcgcccatag cgccggccag agcagcgcct tgaccaggtg ctccagccac agccccaccc
     1861 acggtgcggt gcggtggtgc agtgctagcg gcaacaggag tggccccgcg acgtacagaa
     1921 tgaggagggt ggcgaagccg gcgatcttga ccatcaggag cgccatcagg aagaccagcc
     1981 cgaccagcgc gaccaggtac cagagcggcg cggtgaccgg caccaggccc ttgaggagat
     2041 tcgggaggat atccgtcgtc tggtccgcgc cgatggtcag gatgccgcct gcgacggcgt
     2101 tgcacccacc gaggagccag cccaggagta gacgatagta ggccagcgcg aagagtaccg
     2161 cacccaggcg cgtgaagagc ccaaatggct ccgactcctg gccgaacaga tactgcacga
     2221 gggaaaaagc gaagacgacc aggcacaggg ccaccgccgc ctcgcgcagc gcgtctacca
     2281 gacgctggat gttccccatc gctccaccac tggtgcgcag gtctggcatc aacagcagga
     2341 tctgtgtccc ctgctcgccg agttgctcga acgcgtcgcc gacgatgacg cggtagatcg
     2401 cctggaggag ctggcggagg acgtcctccc agttgagcgc ggccagcaca gcatgcgcga
     2461 gttgcgtcgg ccccggcaac ggaatgctga cactcacaca gagggagccg acacacgcct
     2521 ggccactgcc gttgctgctc tcgttgctca cgccgcctga gtcgctcgct ccactgcccg
     2581 cagtggcggg gctgacacca ccgcaggtcg ggctggcaaa gagagcggga tcgttatggc
     2641 gcgacgtggc ccagtccggc ggcagggcgg tgaagcccga ggtagtccag tcccgtccct
     2701 ggcactggta ttcgccatag agccgggcga cactatcggc gagcgcgccg tccgctgtct
     2761 ggcaggcggg ctggctggac tgctggcaat cgtgggccca gcggtagacc cgcgcctggg
     2821 ccgactccac ccggccgttc ggtccacaat cagcacagcc ctgatcccgg ctggcgtcgt
     2881 agggcacgcc gccaattagc acgatgtggc tgctggtcgg ggtgctaacg actggcggcg
     2941 tgggcgtcgg gacccgattg ggcgacgcgg gtgagggcac agaggtggcc gcgctcgcgg
     3001 gcgccggcac caggatcgcg ccaaggagac cgctgacgag cacagcgagc acgagtgcaa
     3061 tggcgcgaga gcgccatttg cccatcgctg cacgctcctt tccgctctta ccggcgacag
     3121 caggcgtaga cctgccgcaa gaggctcact gcatcaaagt ggtcgctgcc cgcaccgccc
     3181 gtcggcccgc tgccggtgag gatggtgggg ccgatgccga gatgcaggtg cggggcgccg
     3241 ccgtcggcat tgccggtcgc gccgattaca ccgagtgggg tgccagcggt caccagctgg
     3301 ccggcgctga ccagtgggcg gctcgccagg tgggcatacc agtactggag gccatcgtcg
     3361 ccggcaataa gcaccgccca gccaccgagc gtgtcatagt cggcatacgc gacgtgccca
     3421 ccgcgcatgg caagaatggg agtacccgcc ggggcgaaga gatcggcggc gccgggataa
     3481 ctgccccagt gcaggtcgat ggatccggta aagccgacaa ctgggaagac gtacccagtc
     3541 ggcgcgagcg ttgggcctgc tgcggtccgg agaagggcca ggatctgggc cgcataggcg
     3601 tccccgcagg caaagccgtt ggcggcattg gccggatcgc tacaggggtt gtagcggttg
     3661 gcgaaggcga ccagatcggc ggccatggcg tagaccgggc tctgggccac gtactggaac
     3721 cagatcccta gcccagcggt gacggaggga cacgtacccc actggtggcc gagggacgcg
     3781 ggaccgggcg tgcagcccca ggcggcagcg tatggtgcca tggcttccca gatgaggttg
     3841 ccaccattgc cggggacgtt gagcccaacg gtatccagct ggctttcgag ctgccagacc
     3901 acgatgagga gcgggtcaat gccgaagcgc tgaccgaaga ttacgatggt cgcgccatag
     3961 ccacggagcg ggctatcagg cgcgagggca gcgatgaggc ggtcgatccg gacggcgtcg
     4021 gatgggccga agccgaggtt gtggatcgtc ggaccggcgc cggatgggcc gggatacgtt
     4081 cccgaattcc caccaggctg gcttccacca agcgccagcg ccggcagcgc cggcacgacg
     4141 gcgcccgcga gggccgcaag cagcgtcaat cccagcagcg gcaacccgaa caccgccccg
     4201 cagcccagcc caccgagcgc cagccagcgt gtcatcgccc gtcactccgg cgccgaacca
     4261 ccgttggcgg tggttatgcg catcaggcaa cgtgcgccgc gaacatccgt agactggcat
     4321 gtgcgctggc cgtcgcttgc cagagccggc atctgacctg tccgccgtgc agtgcgcggc
     4381 ggcgatgtct gagccgcgag gtctgcaatg gccgcccaga tatcaccggc gtggcgcgcg
     4441 atggagcgct ggcgcagtcg ttgctcggcc gggtcgctcg tgcacagcca gtactcgact
     4501 ggtgtgacta ccagatctac tagcccacgc tgggcgccac tttcggcgta cagatacgcc
     4561 cccgccttga cgcctttgac ggtggagagc cgcccgagcg ccaggcgctc gtcgtccgtg
     4621 agcccgaaca gcgtctgggc ggtgggcaac agcgtggggt gctgctggag gagcaatgcc
     4681 acggcagcgt tctgcaggat ggcctgcccg gtgggcccgg tgtaatcggt gatgaactgg
     4741 ctgaggttga ggctgaccag cccgtgatgc cggccggtcc gcgcgacttc ggccagcagc
     4801 cgggcgctcg ccgggtgctc cagcagtttc cagccctcat cgagaatgag gagaatcggc
     4861 ttcgttgggt cggcgacaac ctgccagatg tgctcgatga gcggcaacat caccagcggc
     4921 accaggcgat cgctcaggtg cttgaagttg aagaccgtga ggagcgccgc actgcggacc
     4981 gtcgtcgggc cgtcgagcaa gcccgcgtag gtaccgtcct cgacataagg caggaggcgc
     5041 tcggccaggc tgtcatagcg ctggcgccgc tcggtatcct cggcggcctg cgcctgatcg
     5101 cgcagccagg catagagatc gcgctcgcgc ggtgtcgggg tggcgccgta caccgcccgg
     5161 caggcgccct cgaggagggc cgtctcggtc gtggtcagct ggcggacccc gtcgtgttcc
     5221 tcaccaagta gtaattggtg caggtcgagc agatactgca ctttcgcctg ccccggctcg
     5281 cggacaccgg ccggcagctg ccagggattg aggcgcaagc cgctgccgag gctgacctgg
     5341 tgcagctccg cgtcgccgac agcggcgacc aggtcggccc agtggtcggt cgagcggtcg
     5401 aggacgagca cccgcccgcc cagcgccagg tgcttgagcg cataggtctg ggcgaacagc
     5461 gttttgccgc tgccgctggt cccacagaca atcaggttgc cgttggggag cgccggatca
     5521 aacatatcca gcggcacgac ttcatgcccg ggctgggaga agccgatgag cggtccaccg
     5581 cgtgttccgg cgctgtggcg caggaacggc aggctatcgg cgagattggt cgtgatggtg
     5641 cggtaggtta gtccggccgg gttgtgggcg agcggcaggg tcgcgcccca ggcggggagt
     5701 tgccagaact gacagcggtc gatgctggtg ccgcccgcgt caccgagcgc acgcacgacc
     5761 tgcccggccg cgccggccag cgcctcgggc gaaccggccc gcacggtgac gaagacggcg
     5821 gtgcggaagg tgcgtagatc gcccgcactc atggccgtca ggagctgttc ggcttcctgg
     5881 taggcctgcc gttgctcgag gtcgggcggc agcccctgct ggtctcgctc ggcgagcacg
     5941 ccgtgccgct gccgcaggcg gcgggttagc gcggtgcgct cccgggcctt gggcaagggc
     6001 acaatatgca gcgcgaagcg gaaggcgacc ccgctcgcgg tgagttgcgc cagccaaccg
     6061 ggaaacgtta gctccggcag agcgcggaga ctgagcgtcg tctcgtagcc ccagtcgagg
     6121 cggagatact cggtccggcg cgtgaggcga ctctgggcca ggcgttcgcg caaggagcgc
     6181 aggtcgggcg gatgcacctc gtgccccgct gcccgcggcg cggtggcgtg ctggctccac
     6241 gcgggattga gacacatcca cagcaggtca atggtcgcct gccggcccag cggtgtagtc
     6301 ggcacgccga gctgcgccag ggtctgctgg acgcccgcca ccgcccggtc gagctcaccg
     6361 agcgcccgcg cctgctgctg gcggtcgcgc acgaggcgcc gctgccgccg ggcgcggcgc
     6421 gcgaacgggc cacgcggcgc cggtggaccg ggtaccacga ccacggcata aaagcgcagg
     6481 tcggggacgg gctggtcggc cagcagctgc tggtaggcgt ccgccagctg cgcgacatat
     6541 gggccaagtg ccggtggtgc ctgatactgc gccaggtgcg cgggtaccca cgccgccgcg
     6601 cggtggggcc gattcatcac caccagctga agccgcacgc ccggcggaag gtaagtgagc
     6661 acggtcgcga actgcgcggc cagttgtgcc gccgtggcgt cgccggccag cgccaggttg
     6721 acgccagtgc ag
//
LOCUS       DASUBI010000636         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_29163, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000636 DASUBI010000000
VERSION     DASUBI010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29163"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="VFU72_12265"
     CDS             <1..2097
                     /locus_tag="VFU72_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424310.1"
                     /translation="RRRLAEALAHAFRAVDRPLLLVLDDLQWTDRETIEWLRYLFHHE
                     ADMCLLVVGTARPEELRSDPLAALTRELGNSDQFTQIALGPLDSHETSTLAADIAGDA
                     LAADQATTIYRETEGNPLFIVETVRAGVPVARGSQESPSGSVPLPPKISAVIAARLAQ
                     LSSPARELAALAATIGRAFSLEVLLGASPEDDETVVAALDELCARHIVRERGQDVYDF
                     SHDKLRDVAYAEISAARRQLLHRRVARAIETVQAHNLEAVSAQLAAHYRQGGLPVEAI
                     RFYELAGERSLRLSAYQDGIAHIQQALELLAGLPASQAHAQQEARLQTALGVALMATK
                     GWPTAEVGEAYGRARALYQPGGDTLDRARLIWGLWGFHIVRGELATARELGERERALA
                     EARNDPNLLLVSGIHLGTSLYHLGDLATARTLLEQAIALYDPEQHRAHASRFGPDLGV
                     FGLAYLSHVLWQLGYPDRALARSRESIALARARAHPYSVAIALDYAAMLAQFRRDRAE
                     VRALADEAIGLSRKQEFAYYLAWGEFLRAWSLDTRSRLAAMREALAALRATGAGLRGT
                     YYFTLLAQAYLEAGQPGDGLNALTEAWDQQREHDERYWEPELHRTQAEILLAQGAPAH
                     VVEQHLQDALASAKSQGAVSLELRAAASLGRLWRDRGMGTEAHELLTALVCRVSEGLD
                     TPDLVETRALLRQIIS"
     gene            2273..3697
                     /locus_tag="VFU72_12270"
     CDS             2273..3697
                     /locus_tag="VFU72_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5424311.1"
                     /translation="MVADQSRATTHAVAAVDPAAVDVLRAQLRGQLLTRTDEGYEAAR
                     AAYNAMIDRRPLLIAGCVDVADVIAGVNFAREQQLPLAIRGGGHNVAGLGSVDDGLLL
                     DLAPMKGIHVDPSMRTARVQAGATWGDVDHATHVFGLAVPSGIISTTGVAGLTLGGGS
                     GHLTRKYGLTIDNLLAVDMVLADGRFITASEDEHPDLFWAIRGGGGNFGVVTSFLFRL
                     HPVDTVIAGPTLWPLEQAAEVMRFYSAFMADAPEDLNGFFAFLTVPPAPPFPEALHMQ
                     RMCGVIWCYTGSEEQAASVFEPVRAFGPPALYGVHALPYPALQGAFDALYPPGLQHYW
                     KADFVRALSDEAIARHIEHGSQVPTLQSTMHLYPIDGAAHRLGTHDTAFSYRDARWSE
                     VIVGASPNPADAQRIKDWAVDYWNAVHPYSEGGAYVNFMMMDEGGERIKATYRDNYER
                     LVAIKRKYDPTNLFHVNQNIRPSA"
BASE COUNT          564 a         1314 c         1283 g          589 t
ORIGIN      
        1 cggcggcgtc tcgccgaggc cttggcgcac gctttccggg cggtcgaccg gccgctgctg
       61 ctggtgcttg acgatctaca gtggaccgat cgcgagacca tcgagtggct gcggtacctc
      121 ttccatcatg aagccgatat gtgcctgctg gtggtgggca cggctcgccc ggaggagctg
      181 cgcagcgatc cgctcgcggc gctcacgcgc gagcttggca acagcgacca attcacccaa
      241 atcgcgctgg gaccgctcga tagccacgaa acctcgaccc tggcggccga catcgccggc
      301 gacgcgctcg ccgccgacca ggcgacgacg atctaccgcg agaccgaggg taatccgctc
      361 ttcatcgtgg agacggtgcg cgcgggggtg ccggtcgcca gaggcagtca ggagtccccg
      421 tccgggagcg tgccgctccc gccgaagatc tccgcggtga tcgcggcacg cctggcacaa
      481 ctctcatcgc cggcgcgtga gctggcggct ctcgcggcga cgatcggacg cgccttcagc
      541 ctggaggtgc tgctcggcgc gagccccgag gatgacgaga ccgtggtggc ggcgctcgat
      601 gagctctgcg cgcggcacat cgtgcgcgag cgcggccagg acgtctacga cttcagccac
      661 gacaagctgc gcgacgtggc ctacgccgag atcagcgcgg cccggcggca gctcctgcac
      721 cgtcgcgtgg cgcgcgccat cgagactgtc caggcccaca atctggaagc cgtcagcgcc
      781 caattggccg cgcactaccg gcagggcggc ctgccggtag aggcgattcg cttctacgag
      841 ctggccggtg agcgctcgct gcgcctctcc gcctatcagg atggcatcgc gcacatccag
      901 caggcgctgg agctgctggc cgggctgccg gctagccagg cgcacgcgca gcaggaagcg
      961 cggctccaga ccgcgctggg cgtggccctg atggcgacca aaggctggcc cacggcggaa
     1021 gttggtgaag cctacggacg cgctcgggcg ttgtatcagc ccggcgggga tacgctcgat
     1081 cgcgcgcggc tgatctgggg tctgtggggc ttccacatcg tccgcggcga gctcgccacg
     1141 gcccgcgagc tcggggagcg cgagcgggcg ctggccgaag cccggaacga tcccaacctg
     1201 ctcctggtgt ccggaattca cctgggaacc agcctctacc acctggggga tctcgccacg
     1261 gcgcgcacgc tgctggagca ggccatcgcg ctctatgacc ccgagcaaca tcgcgctcac
     1321 gcctcccgct ttggacccga tctgggcgtg tttggcctgg cctacttgtc ccatgtgctg
     1381 tggcagctcg gctatccaga ccgggccctc gcgcgcagcc gggagagcat cgcgctggct
     1441 cgtgcacgcg cccaccccta tagcgtcgcg atcgcgctgg actacgccgc catgctcgcg
     1501 caattccggc gggaccgggc tgaagtgcgt gcgctggccg acgaggccat cgggctctcg
     1561 cgcaagcagg agttcgccta ctatctggcc tggggggagt tcttgcgcgc ctggtcgctg
     1621 gacacgcggt cacgtttggc cgccatgcgc gaggcattgg ccgcgctgcg ggcgacggga
     1681 gccgggctgc ggggaaccta ttacttcacc ctgctcgctc aggcctatct ggaggccggg
     1741 cagccgggtg acggcttgaa cgcgttgacc gaggcctggg accagcagcg cgagcacgac
     1801 gagcgctact gggagccgga gctgcaccgg acgcaggcgg agatccttct cgcacaaggg
     1861 gcgccggcac acgtggtcga gcagcatctc caagacgcgc tcgcctccgc caagagccag
     1921 ggagccgtca gcctggagct gcgcgcggcg gcgagcctgg gccgcttgtg gcgcgatcgg
     1981 gggatgggca cggaggccca cgagcttctc accgccctgg tatgccgggt ctccgaaggg
     2041 ctcgacacgc ccgacctggt agagacgcgc gcgctgctgc ggcagatcat ctcgtaggcg
     2101 ccgacgccga acggttcctg gcccgatttt cccaccgaaa tcgctcaccg cgaacgctcc
     2161 agcgaacgct ttgcgaacgc cgcacactct atcctcgacc tatgctcagt cgtatcgcga
     2221 gccactggac gcgttcgcgg attccggtgg ctcctactca gggagggtca tcatggtggc
     2281 tgatcagtcc agggcgacga cgcatgcggt ggcggcagtt gacccggcgg ctgtcgacgt
     2341 cctccgcgct cagctgcgcg gtcagctcct cacccggacg gacgagggct acgaggccgc
     2401 ccgcgcggcc tacaacgcga tgatcgaccg gcgcccgctg ctcatcgcgg gctgcgtgga
     2461 cgtcgcggac gtcatcgccg gggtcaactt cgcccgcgag cagcaactgc cgctcgccat
     2521 tcgcggcggc gggcacaacg tcgctggttt ggggagcgtg gatgacggcc tgctcctcga
     2581 cctcgcgccg atgaagggga tccacgtcga tcccagcatg cgtaccgccc gcgtccaggc
     2641 cggcgccacc tggggcgacg tcgaccacgc cacccatgtc ttcggcctgg ccgtgccgtc
     2701 cggcatcatc tcgactaccg gcgtcgccgg gctgactctc ggcggcggct ctggtcatct
     2761 gacgcgcaag tacggcctga ccatcgacaa tctgctggcg gtggacatgg tcctggccga
     2821 cggccggttt atcaccgcca gcgaggatga gcacccggat ctcttctggg ccatccgtgg
     2881 cggcggcggc aacttcggcg tcgtcacctc gttcctcttc cgcctgcatc cggtagacac
     2941 ggtcatcgcc gggccgacgc tctggccgct cgagcaggcg gcggaggtga tgcgcttcta
     3001 cagcgcgttc atggcggacg cgccggagga tctgaacggc ttcttcgcct ttctcacggt
     3061 gccgcccgcg ccgcccttcc cggaggcgct gcatatgcag cgcatgtgcg gcgtgatctg
     3121 gtgctatacg ggatcggagg agcaggcggc ctcggtgttc gagccggtgc gggcctttgg
     3181 gccgcccgcg ctctacggcg tgcatgccct gccctatccc gcgctgcagg gcgcgttcga
     3241 tgccctctat ccgccgggcc tgcagcacta ctggaaggcc gacttcgtcc gtgcgctgag
     3301 tgacgaggcg atcgccaggc acatcgagca cggctcgcaa gtcccgaccc tgcaatcgac
     3361 catgcatctc tacccgatcg acggggcggc gcaccggctg ggcacgcacg acaccgcctt
     3421 cagctatcgt gatgcccgtt ggtcggaggt catcgtcggc gccagcccga atcccgcgga
     3481 cgcgcagcgc atcaaggact gggccgtcga ctactggaac gccgtccacc cctattcgga
     3541 gggcggcgcc tacgtcaact tcatgatgat ggacgagggt ggggagcgca tcaaggccac
     3601 ctatcgcgac aactacgagc gcctggtggc gatcaagcgc aagtacgacc cgacgaacct
     3661 cttccacgtc aatcagaaca tccggccgag cgcctgaccc aggcgagcgc ggcccggccg
     3721 ggtagccggc cgggccgcgc ggccggcacc
//
LOCUS       DASUBI010000637         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_318124, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000637 DASUBI010000000
VERSION     DASUBI010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318124"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFU72_12275"
     CDS             complement(<1..978)
                     /locus_tag="VFU72_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5424312.1"
                     /translation="MSQRLRVGVIGGGILGLTAAYRLAKAGHDVILWERAPRLGGQAA
                     AFPVLDTALEYFYHHLFTSDRDIAALMEELGIGDKLLWLPSNVGFYAGGKIYPLNGAL
                     DLLRLDTIPLSDRLRIGLTTLYLQKTRNWAKFERVTAARWLQRAVGRRAFDAVWGAQL
                     RAKFGPRYDQVAMVWFWNKIFLRTQSRRSPLEQERLGYIQGSFNVLIDRLAQAIGEQG
                     GVVRAGVGTDALERRGDGWVVRTSEGEGVERDVLIASVPSPILSRLVPELPEEYRAKL
                     SGAVYQGAVTMLLQSRQPLSNIYWLNIGDPSLPFTGIIEHTNFIGPEHYG"
     gene            complement(975..1949)
                     /gene="secF"
                     /locus_tag="VFU72_12280"
     CDS             complement(975..1949)
                     /gene="secF"
                     /locus_tag="VFU72_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5424313.1"
                     /translation="MTLNIVGKRYWWFALSLLIILPGVFFLATEGLRLSIDFTGGTLW
                     EMQFSHPVQPGAVKDVLAQYGYGNAEVQTTQDNGVQIRMKELKEASDTKTQIEQALQA
                     KFGPINEQTASFESVGPTLGNAIRNRAIVAVGIASIGILLYIAYAFRKTQNPFAFGIC
                     ALLAMLHDVLVVVGIFSFLGWWKGVEVDALFVTALLTVIGFSVHDTIVVFDRIRENLA
                     HRAGRTFEEVVNYSLDQTLVRSLNTSLTVIFTLLALFLLGGTTIKWFVLALLIGIFSG
                     TYSSIFNASQLLVVWENRELQHFFARLRGRSPRPAAGSGAGTGRVASR"
     gene            complement(1990..3486)
                     /gene="secD"
                     /locus_tag="VFU72_12285"
     CDS             complement(1990..3486)
                     /gene="secD"
                     /locus_tag="VFU72_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5424314.1"
                     /translation="MRFKPWYTLILIVILTLIAGWVDLPAQTLNPFGIKDGVSIHKGL
                     DLQGGLQVVLQAKPPKGQTLTSGTLQGERDTIERRVNGLGVSEPLIQTRGNDQIIVEL
                     PGVKDPQNAVKVLQQTALLEIIDTQGQALPVGTVVNTTAGSAADFESGKATPTANGTP
                     GATPNATPGATPEASPVAGATGSTTNPNDQNNPAANGPVYQTIVTGADLKDAYPSTDQ
                     FGSVVVGFELKSDAANKFYQYTSTHVGQPMSIVVDKHVINTATIQSAIQNTGQISGLT
                     VQEVNDLSVQLKAGALSVPLDVIQSRTVGPTLGQDSIDRSITAGLAGLAIVGLFMILF
                     YRVPGVLSVIALMIYTGIAFALFKSIPVVLTLAGIAGFILSIGMAVDANVLIFARLKE
                     ELRKGHTVHRAVEAGFDHAWPSIRDSNFSTMITCVILYWFGRYTGASIITGFALTLGI
                     GVAVSLFTAITVTRTLLRVMLNSGWFHNEWWFGVEKHEIPTTAPAAGD"
BASE COUNT          627 a         1172 c         1336 g          622 t
ORIGIN      
        1 gccgtagtgc tccgggccga tgaagttggt gtgctcgatg atcccggtga aggggagcga
       61 cggatcgccg atgttcagcc agtagatgtt cgagagcggc tggcgcgact gcaagagcat
      121 ggtcaccgcg ccctggtaga cggcgccgga gagcttggct cggtactcct ccggcagctc
      181 cggcaccagc cgcgagagga tcggcgaggg cacgctggcg atcagcacgt cgcgctcgac
      241 cccctcgccc tcgctggtgc gcacgaccca accgtcgccg cgccgctcca gcgcgtcggt
      301 gccgacgccg gcccgcacca cgcccccctg ctcgccgatg gcctgggcga gccggtcgat
      361 caggacgttg aagctcccct ggatgtagcc cagccgctcc tgctccagcg gcgagcggcg
      421 cgattgggtg cgcaggaaga tcttgttcca gaaccagacc atggccacct gatcgtagcg
      481 cggtccgaac ttggcgcgca gctgggcgcc ccagaccgcg tcaaaggcac gccggccgac
      541 cgcgcgctgt agccaccggg cggcggtgac gcgctcgaac ttggcccagt tgcgtgtctt
      601 ctgcaggtag agcgtggtca ggccgatccg caggcggtcg ctgagcggga tggtgtcgag
      661 ccgtagcagg tcgagcgcgc cgttgagcgg gtagatcttg ccgccggcgt agaagccgac
      721 gttggagggg agccagagga gcttgtcgcc gataccgagc tcctccatca gcgccgcgat
      781 gtcgcggtcg ctggtgaaga ggtggtggta gaagtactcg agggcggtgt cgagcaccgg
      841 gaacgccgcc gcctggcccc cgagccgcgg cgcgcgctcc cagaggatga catcgtggcc
      901 ggctttggcc agccggtagg cggcggtcag tcccaggatg ccgccgccga tgacgccgac
      961 gcggagtcgc tggctcaccg gctggcgacc cgtccagtcc ccgctccgga ccccgcggcc
     1021 ggccgcggcg agcgtccgcg caggcgggcg aagaagtgct gcaactcccg gttctcccag
     1081 acgaccagca gctggctggc gttgaagatc gaggagtacg tgccgctgaa gatgccgatc
     1141 agcagagcga gcacgaacca cttgatcgtc gtgccgccga gcaggaagag cgccagcagg
     1201 gtgaagatga ccgtcagcga ggtgttgagc gagcgcacca gcgtctgatc gaggctgtag
     1261 ttgacgacct cttcgaaggt gcgtccggcg cggtgggcca ggttctcgcg gatgcggtcg
     1321 aagaccacga tggtgtcgtg gaccgagaag ccgatgacgg tcaagagcgc ggtgacgaag
     1381 agggcgtcga cttcgactcc cttccaccag cccaggaagg agaagatgcc gaccaccacc
     1441 agcacgtcgt gcagcatcgc cagcagcgcg cagataccga aggcgaacgg gttctgcgtc
     1501 ttgcggaagg cgtaggcgat atacaggagg atgccgatcg aggcgatgcc gaccgccacg
     1561 atggcgcggt tgcggatggc gttgcccagc gtgggaccga ccgactcgaa gctggcggtc
     1621 tgctcattga tcgggccgaa cttggcctgc agcgcctgct cgatctgggt cttggtgtcc
     1681 gaggcctctt tcagctcctt catgcggatc tgcacgccgt tgtcctgggt cgtctggacc
     1741 tcggcattgc cgtagccgta ctgcgccagc acgtccttga ccgcgccggg ctgcaccggg
     1801 tggctaaact gcatctccca cagggtgcca ccggtaaagt cgatcgagag tcgcagcccc
     1861 tcggtcgcca ggaagaagac tcccggcaga ataatcagca gtgacagcgc gaaccaccag
     1921 tagcgcttgc cgacaatatt gagcgtcacg tccttctacc tcgctctcgg ccagcggccg
     1981 aattcgtcgc tagtcaccag ccgccggagc ggtcgttggg atctcgtgct tctcgacgcc
     2041 gaaccaccat tcgttgtgga accagccgct gttgagcatc acacggagga gtgtgcgcgt
     2101 gacggtgatc gcggtgaaca agctgaccgc cacgccgatg cccagcgtca gggcgaagcc
     2161 ggtgatgatg ctggcgccgg tgtagcggcc gaaccagtag agaatgacgc aggtgatcat
     2221 ggtcgagaag ttggagtcgc ggatcgacgg ccaggcgtgg tcgaagccgg cctcgaccgc
     2281 gcgatggacc gtgtggccct tgcgtagctc ctctttgagt cgcgcgaaga tcagcacgtt
     2341 ggcgtcgacc gccatgccga tcgagaggat gaagccggcg atgccggcca gcgtcagcac
     2401 gaccggaatc gacttgaaga gggcgaacgc gatgccggtg tagatcatca gcgcgatcac
     2461 cgagagcacg cccggcacgc ggtagaagag gatcatgaag agcccgacga tcgccagccc
     2521 ggccagaccg gccgtgatgc tgcggtcgat cgagtcctgg ccgagcgtcg gcccaacggt
     2581 gcggctctgg atcacgtcga gcggcacgga gagggcgccg gccttgagct gcacgctcag
     2641 gtcgttgacc tcctgcacgg tcaggccgct gatctggccg gtgttctgaa tggccgactg
     2701 gatggtggcg gtgttgatca cgtgcttgtc gaccacgatc gacatcggct ggccgacgtg
     2761 ggtgctggtg tactggtaga acttgttggc cgcgtcggac ttcagctcga agccgaccac
     2821 caccgagccg aactggtcgg tgctggggta ggcgtctttg agatcggcac cggtgacaat
     2881 cgtctggtag accgggccgt tggcggccgg gttgttctgg tcgttcgggt tcgtggtgct
     2941 gccagtagcg ccggcgacgg ggctggcctc aggcgtggcc cccggcgtgg cgttcggagt
     3001 cgccccgggc gtcccgttcg ccgtgggggt ggccttgccg ctctcgaagt cggccgcgct
     3061 gccggcggtc gtgttgacga cggtgccgac cgggagcgcc tggccctggg tgtcgatgat
     3121 ctccagcagc gcggtctgct gcaggacctt gaccgcgttc tgcggatcct tgacgccggg
     3181 cagctcaacg ataatctggt cgttgccgcg tgtttggatc agcggctcgc tgacgcccag
     3241 cccgttgacg cgccgctcga tggtgtcgcg ctcaccctgc agcgtgccgg aggtcagcgt
     3301 ctgccccttg gggggcttgg cttgcagcac gacctgcaaa ccgccttgca gatcgagtcc
     3361 cttgtggatc gagacgccgt ccttgatgcc gaacggattc agcgtctgcg ccgggagatc
     3421 gacccagccc gcgatcaggg tgaggatgac gatcaggatg agggtgtacc agggcttgaa
     3481 acgcaacgcg catctcctca aggctgccgg acacaccgcc gggcaacagc agccgcggtg
     3541 acacgaactc ccgcatggcg ggtgacgaac agcgagtaag tatagcgagc gggaaggtgc
     3601 ggtgcaaaag ctcgacccac gattggtgac gaggcggcac agcacgcgcg gtgcggcgtg
     3661 atgcgacgcg gcgcgtggcc gctggcggcg gtgatgagcg ccgacggtct gtcacggctc
     3721 ggctcctcgc tgacggcagt ggcgataccc tggttcg
//
LOCUS       DASUBI010000638         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_822021, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000638 DASUBI010000000
VERSION     DASUBI010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822021"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..987)
                     /locus_tag="VFU72_12290"
     CDS             complement(238..987)
                     /locus_tag="VFU72_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424315.1"
                     /translation="MRGRGGEGERGRGERRLVSACLALGGALLALAVVAAACSGPVPP
                     PSGVPGATAALVVARPSATPTTPAPTPTATPTPSPTPTPTPSPTPTPSPTPTPAPTPT
                     PSPTPTPTPPPATPTPIPSVLALGAQQSYPNGTSVTVFAVQPAPSGTGDLIPGRDERL
                     LALDIQVCAGRQVDGLAVSPQEFALELSDGSQHAGADGGRAPVLRSDGIIPGQCVRGW
                     VTVPAPKSPDPRYIVGHIPSYDPARWVFPGL"
     gene            complement(1122..1943)
                     /locus_tag="VFU72_12295"
     CDS             complement(1122..1943)
                     /locus_tag="VFU72_12295"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5424316.1"
                     /translation="MVSTSRPVGELLRMWRRRRRLSQLEMALEAEVSARHLSFLETGR
                     SLPSRQMLLHLAEQLAIPLRERNTLLVAAGYAPVYPATPLHDPALDAAREAVELVLGG
                     HEPYPALAVDRAWNLVAANRSTGFLLSEVAPALLQPPVNVLRVSLHPEGLAPRIVNLG
                     EWRRHLLARLQQQIEASADPELEAIYQELRGYPAPQPEMGSPARNDDQPHPALVVPVR
                     LRAGSEVLSLFSTTTVFGTAVDITLAELTIESFFPADTATAEALRRLAHQESPGS"
     gene            2077..2454
                     /locus_tag="VFU72_12300"
     CDS             2077..2454
                     /locus_tag="VFU72_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5424317.1"
                     /translation="MSDLNGLIERYFAIWNEPDPARRDELIGRTWSDDASYLDPMLSA
                     EGPDGIQTMVASVQEQFPGHRFRRVSEIDSHHDRLRFAWELVNPASAPVVRGSDYGLL
                     APDGRLRAITGFFEQPAPPSEAP"
BASE COUNT          330 a          874 c          929 g          328 t
ORIGIN      
        1 gggagacgag caggctcgcc agcagcacga gcgcgacggc gacggtgtgg gcacggcagc
       61 ggcgcgccac ggcgatcctt tccggtcggt tgctggttcg gctcaagctg ccgggcccga
      121 gcaccccgcg gcgcgccgct cgtcacgctt gagcctggtt gcccctggtc gactgttcgc
      181 gccgccgagc ataccagaga cgagcggcca gccggcgatc gcattggctc accgcctcta
      241 cagaccgggg aagacccagc gcgccggatc gtagctgggg atatggccga cgatgtagcg
      301 cggatcgggc gacttcggcg ccggcaccgt aacccagccg cgcacgcact ggcccgggat
      361 gatgccgtcg gagcgcagca ccggcgcccg cccgccatcg gccccggcgt gctggctgcc
      421 gtccgacagc tccagcgcga actcctgtgg actgaccgcc agaccatcga cctgccgccc
      481 ggcgcagacc tggatgtcca gcgccagcag ccgctcgtcc cgtccgggta tcagatcgcc
      541 cgtgccgctg ggcgccggct gcaccgcgaa gacggtgacg ctggtgccgt tggggtagct
      601 ctgctgcgcc ccgagcgcca gcaccgaggg gatcggcgtc ggcgtggctg gcggcggcgt
      661 gggagtcggc gtcggactcg gggtcggggt cggcgccggc gtgggagtcg ggctcggcgt
      721 cggcgttggt gagggtgtcg gcgtcggcgt cgggctgggc gtgggggtgg ccgtgggcgt
      781 cggcgcgggt gtggtaggcg tggccgaggg gcgcgcgacg acgagcgccg cggtcgcgcc
      841 ggggacaccg ctcggcggcg gcaccgggcc gctgcacgcg gcggccacga cggccagcgc
      901 cagcagcgcg ccgccgagcg ccaggcacgc ggagacaaga cggcgctctc ctcgcccccg
      961 ctcgccctcg cctccccggc cgcgcatccc ctggctcgct cctccgtcga tcatgcccgc
     1021 cgacccgctg cagcgccgca cgcacctgct tctgtgtacg tgtacaccgc ccgtgatggc
     1081 tcacgcatcg ggccatagtc acgtttctgc cgcctggccg ctcagctgcc cggcgactcc
     1141 tggtgcgcca ggcgacggag ggcctcggcg gtcgcggtat cggcggggaa gaacgactcg
     1201 atggtcagct cggccagggt gatgtcgacg gcggtgccga agacggtggt ggtgctgaag
     1261 agggagagca cctccgagcc ggcgcgcagg cgcaccggca ccaccagggc cgggtggggc
     1321 tggtcatcat tgcgcgcggg gctgcccatc tccggctggg gagccggata gccgcgcagc
     1381 tcctggtaga tcgcctccag ctccgggtcg gcgctggcct cgatctgttg ctggagccgg
     1441 gccagcaggt gccggcgcca ctcgccgagg ttgacgatgc ggggcgccag gccctccggg
     1501 tgcaggctga cgcgcagcac gttgaccggc ggctggagca gcgcgggcgc gacctcgctg
     1561 agcaggaagc cggtggagcg gttggcggcc accagattcc aggcgcggtc gacggccagc
     1621 gcggggtagg gctcgtggcc gcccaggact agctcaaccg cctcgcgggc ggcgtccagg
     1681 gcggggtcgt gcagcggcgt ggcggggtag accggggcgt agccggccgc gaccagcagc
     1741 gtgttgcgct cgcgcagcgg aatcgcgagc tgctcggcca ggtgcaacag catctgccgg
     1801 ctggggagcg agcggccggt ctccaaaaag ctgagatgcc gcgccgagac ctcggcctcc
     1861 agcgccatct cgagctggct gaggcggcgg cgccgccgcc acatgcgtag caactctccg
     1921 accggccggc tcgtgctcac catgcgcgca tgctaacgcc acggggggcc ggttgaccat
     1981 tacctgcgag gtaatcgact tgccgcaccc gcgcctccta cgatgattcc agcgtaaccg
     2041 gcggcgccag ccgcgagcca gcagatggag ggatccatga gcgacctgaa cggcctgatc
     2101 gagcgctact tcgcgatctg gaacgagccc gatcccgcgc ggcgcgacga gttgatcggc
     2161 cgcacctgga gcgacgacgc cagctatctc gatccgatgc tgagcgccga ggggccggac
     2221 ggcatccaga cgatggtcgc gtcggtccag gagcagttcc ccggccaccg cttccgccgc
     2281 gtgagtgaga tcgacagcca ccacgaccgg ctgcgcttcg cctgggagct ggtcaacccg
     2341 gccagcgccc cggtcgtgcg cggcagcgac tacgggctgc tcgcgcccga cggtcgcctg
     2401 cgcgcgatca ccggcttctt cgagcagccc gccccgccgt ccgaggctcc gtaggccggg
     2461 c
//
LOCUS       DASUBI010000639         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_133057, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000639 DASUBI010000000
VERSION     DASUBI010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133057"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VFU72_12305"
     CDS             complement(<1..1816)
                     /locus_tag="VFU72_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5424318.1"
                     /translation="MKSAPALKQPRHRHRWQVQRPLALPAGGIAALPALTLLAILAVL
                     VWVGLRPLATPAVVPATAPATTFSAERAMAHLRVIAAQPHAIGSPENAAVRQYLVEQV
                     RALGLEPEIQTTSQAVAEGEEAFRAGVVDNIIVRVPGMRSTGAIAIDAHYDSVNTGPG
                     AGDCGSCVVTALEALRAVVAGPALQNDLLFVFSDGEERHMLGAAAFNQQHPLAGDVRL
                     ALNFEAQGSGGPAFLYVTSPDDRWLVSQFLAVAPDASAYSLLASISDLYPAGRFDSDL
                     GWYTERGSQGLGVIFTADTPAYHTDRDNPNVIDPGSIQQEGAYTLALARHFGNLDLTT
                     MPAAAGDSVFFTVWPGVVVHYPFSWTLPLAAVITVLVAGLLFLGVRRQELTSGGLVLG
                     LVSFVTGSLVTVLAATGIWFAVRTLNGAYQVHMAGSYQSGLYQVALILASIALMTGFY
                     TLLGRKVRRHNLVAGALLGWTLLLWLLSLTVPGISYLATWPLLFALLPPAWSALACSR
                     ASQPWWQVLILAIAAVPALVLLPGTLHQMAALINRFEGVLDFPLLGIVMVVVAPLLAL
                     FLPHLHFLAGDPGGPRRWAVPAATALVALALIGWGNATS"
BASE COUNT          362 a          766 c          847 g          308 t
ORIGIN      
        1 cggaggtcgc gttgccccag ccgatcagcg ccaacgcgac gagcgccgtt gccgccggca
       61 ctgcccaacg ccgtgggccg ccagggtcac cggccaggaa gtgcaggtgc ggcaggaaga
      121 gcgccagcag cggcgcgacc acgaccatca cgatgcccag cagcgggaag tcgagcacac
      181 cttcgaagcg gttgatcagc gccgccatct gatgcagcgt ccccggcagc agcacgaggg
      241 ccggcacggc cgcgatggcg aggatcagca cctgccacca gggctggctc gcgcgcgagc
      301 aggccagcgc cgaccaggcc ggcggcagca gcgcgaagag aagcggccag gtcgccaggt
      361 agctgatgcc cggcaccgtc aggctgagca gccagagcag gagcgtccag ccgagcagcg
      421 cccccgccac gagattgtgt cgccgcacct tgcggccgag cagggtgtag aaccccgtca
      481 tcagcgcgat gctggcgagg atcagcgcga cctggtagag cccgctctgg tagctgccgg
      541 ccatgtgcac ctggtaagcg ccgttgagcg tgcggacggc gaaccagatg ccggtggcgg
      601 ccagcacagt caccagtgag ccggtgacga agctcaccag gcctaacacc agcccgccgg
      661 aggtcagttc ctggcgccgg acgccgagga agagcagccc ggcgaccagc acggtgatga
      721 ccgcagccag cggtagcgtc cagctgaagg ggtagtggac cacgacgccg ggccagaccg
      781 tgaagaagac cgaatctccc gcggccgccg gcatcgtcgt caggtccagg ttgccgaagt
      841 gacgtgccag cgccagcgtg taggcgccct cctgctggat gctgcccggg tcgatcacgt
      901 tcgggttgtc gcggtcggtg tggtaggccg gcgtatcggc ggtgaagatg acgccgagcc
      961 cttggctgcc ccgctccgtg taccagccga gatcggaatc gaagcgcccg gccgggtaga
     1021 ggtcggagat gctggcgagc aaggagtagg cgctggcgtc cggcgcgacc gcgaggaact
     1081 gagacaccag ccaacgatcg tccgggctgg tgacgtagag gaaggccggg ccgcccgagc
     1141 cctgcgcctc gaaattgagc gccagccgca cgtcgccggc cagtggatgc tgctggttga
     1201 aggcggcggc gcccagcatg tgccgctcct cgccgtcgct gaagacgaag aggaggtcgt
     1261 tctggagcgc tggcccggcg acgacggcgc gcagcgcctc gagcgccgtc accacgcagg
     1321 agccgcagtc gcccgcgccg gggccggtgt tgaccgagtc gtagtgggcg tcgatggcga
     1381 tggcgcccgt gctccgcatg cccggcaccc gcacgatgat gttgtcgacg acgccggcgc
     1441 ggaaggcctc ctcgccctcg gccacggcct gggaggtcgt ctggatctcc ggctccagcc
     1501 cgagcgctcg tacctgctcc acgagatact gccgcacggc cgcgttctcc ggcgatccga
     1561 tcgcgtgcgg ctgggcggcg atgacgcgga gatgcgccat ggcgcgctcg gccgagaagg
     1621 tcgtggccgg cgccgtcgcc gggacgacgg ccggcgtcgc gagcgggcgc agcccaaccc
     1681 agacgagcac ggcgagtatg gccagcaggg tgagcgccgg cagcgcggct atccccccgg
     1741 ccggcagcgc cagcggccgc tgaacctgcc agcggtggcg gtgacgcggt tgctttagcg
     1801 cgggagcgga tttcatcgtt gtcagtcctt acgccgcatt gttcgccagc cggcgcaagc
     1861 cggtgggcga gcgcgtgcct gcgcctcaag tgttcgcctg ggggcgtttc gaaggggtga
     1921 attcgggccg gcgggcgtga caataccgtg acgcgcgccg gattcctccc cgagcgcggg
     1981 tggcgcggga gcgactccgt ggctggcgag gaacacgggt gacgcgtggg gcacggatcc
     2041 ttcgactgcg ctcagagcct gccctgagcg tgtcgaagga tccgtcctct cccggcaccc
     2101 aacgtgttcg tcaggcagcc cctgttcccg ctcagccggt ccggaggctc gcctcccagc
     2161 tcagggcgtc gccttccggg aggtcggtca cgccgagccg ctgcagcagg tggaggatct
     2221 caccgcggtg ggtcatgttg tgggtgatga cgtggacgat ggtgccgccg aacgtcttgg
     2281 gcc
//
LOCUS       DASUBI010000640         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_494806, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000640 DASUBI010000000
VERSION     DASUBI010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494806"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFU72_12310"
     CDS             complement(<1..395)
                     /locus_tag="VFU72_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424319.1"
                     /translation="MADASDERAIHYRSDQRRRRDFFVQRAPGLRLFVREVRGTEPAR
                     GVPLLLLHGARVPGVASFDLDVPGGSLAADLAQSGRVVYLMDAHGYGFSTRPAAMAQP
                     RTGNPPLARAPQIVRDVAAVVEAIVERTG"
     gene            complement(408..1370)
                     /locus_tag="VFU72_12315"
     CDS             complement(408..1370)
                     /locus_tag="VFU72_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEU5424320.1"
                     /translation="MWRSARLARWLALAATVGMFAVLVMGATVTNTGSAEGCGRSWPL
                     CHGKFIPSFAVATAIEFSHRLVTSVESLLVFALAAVALLGWRQRREVRVLVPLMVGTL
                     LLQAGLGAWAVLAPQQPAVLAAHFGVSLTAFASTLLTAVFFWQLEGGERQRELPLPRH
                     LRGAVFGVIGYIYLLVYLGAYVRHSGASLACGGWPLCAGQVFPGFHGYAGIEFAHRLA
                     AVAGTLLVVGLAAWTRRGRTARPDLYRGSLLALGLILVQSLSGALVVWTHLDLFSALT
                     HAAIVTLLFGSLTYVAYQTLPLPALARRATREPVRAVRVAPAER"
     gene            1812..>2832
                     /locus_tag="VFU72_12320"
     CDS             1812..>2832
                     /locus_tag="VFU72_12320"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5424321.1"
                     /translation="MEAKDFVDVKPNPIQPAEGRLGILLVGLGAVASTFISGVEHARR
                     GSGRPVGSLTQMGTIRLGKRTENRSPLIREFVPLANLDDIVFGAWDPFPDNAYEAALK
                     CGVLDRHEHIEPIADFLKSIHPMPAAFDQYYVKRLDGPNVKRGTKMEMAEQIRADIRN
                     FKSANACDRLVMIWCASTEIFMEEGRSHGNLDSFEAALEVDDETIAPSMIYAYAALME
                     GVPFANGAPNLTADIPALALLANELGVPICGKDFKTGQTMIKTVLAPMLKARMLGLSG
                     WYSTNILGNRDGEVLDDPESFKTKEESKLGVLEYILQPQLYPELYSDVYHKVRINYYP
                     PRGDNK"
BASE COUNT          513 a         1023 c          931 g          365 t
ORIGIN      
        1 ctgcccgtgc gctccacgat cgcctcgacg acggccgcca cgtcgcggac tatctgtggc
       61 gcccgcgcca gtggcggatt gcccgtgcgg ggctgggcca tggccgccgg acgcgtcgag
      121 aagccgtagc cgtgcgcgtc catcaggtag accacccggc cgctctgcgc cagatcagcc
      181 gccagtgagc cgcccggcac gtcgaggtca aaggacgcca cgccgggtac gcgcgccccg
      241 tgcagcagca ggagcggcac gccgcgcgcc ggctccgtgc cgcgcacctc gcgcacgaag
      301 aggcgcagac cgggcgcgcg ctggacgaag aagtcgcgcc gccggcgctg atcgctccgg
      361 tagtgaatcg cgcgctcgtc tgacgcgtcc gccatcgcgc tctcccttta tcgctcggcc
      421 ggcgcgaccc gtacggcgcg caccggctcc cgcgtggcgc ggcgcgcgag ggcgggcagc
      481 ggcagcgtct gataggcgac gtaggtcagg ctgccgaaga ggagcgtgac gatggccgcg
      541 tgcgtcagcg ccgagaagag atcgagatgg gtccagacga ccagcgcgcc gctcagcgat
      601 tgcaccagga tcagccccag ggccagcagg ctgccgcggt agagatcggg gcgggccgtg
      661 cggccgcgcc gggtccaggc cgccagcccc accaccagca gcgtgccggc cacggcggcc
      721 agccggtggg cgaactcgat gccggcgtag ccgtggaagc cggggaagac ctgcccggcg
      781 cagagcggcc agccgccgca ggccaggctg gcgccgctgt ggcgcacata ggcgccgaga
      841 tagaccagca ggtagatata gccgatcacg ccgaagacgg cgccgcgcag gtgacgcggc
      901 agcggcagct cgcgctgccg ctcgcctcct tcaagctgcc agaagaacac ggcggtcaag
      961 agcgtgctgg cgaaggcggt cagcgagacg ccgaagtgcg ccgccagcac cgccggctgc
     1021 tgcggggcca gcacggccca ggcgccgagc ccggcctgca ggagcagcgt gccgaccatc
     1081 agcggcacca gcacccgcac ctcgcgccgc tggcgccagc cgagcagcgc cacggcggcc
     1141 agggcgaaga ccaggaggct ctcgacgctg gtgaccaggc gatggctgaa ctcgatggcg
     1201 gtggccacgg cgaagctggg gatgaacttg ccgtggcaga gcggccagga gcggccgcag
     1261 ccctcggccg agccggtgtt ggtcacggtg gcgcccatca ccagcacggc gaacatcccc
     1321 accgtagccg ccagtgccag ccagcgcgcc agccgcgccg accgccacac atcacccctc
     1381 aaagcatcgg tgcgcatcgt cgtcctcagt cagccaggcc aagttcctcg cgcaagtgta
     1441 ccgcgcgccc gccgggtggc ggtgtagggg tgccggccgt ccctcttcag atcgcgcgct
     1501 aacccgctgg gatagtcccc gcatcaccgg ccagtcccga ccgggccctc gcccctacca
     1561 tcgataccca gcaggggtgc atacccggcc ctggcccatt gccttctaac gattttttcg
     1621 ttaggatgtc cgccatggaa gaaagaccga acaccgctga gcggatcatc aagcggttcg
     1681 gagggcagag cgcgctagcg gcgctgctcg gcaagcgtca gagcacgatc gaacactggg
     1741 cgaagacagg gcgggtgccc gcgcaatggc acgtgccact catatcgctg gctcgccaga
     1801 gaggtgtgat cttggaagca aaagacttcg tcgacgtcaa gcccaacccg atccaaccgg
     1861 cagagggccg cctcggcatc ctgctcgtcg gcctcggcgc cgtcgcctcg acgttcatca
     1921 gcggcgtcga gcacgcgcgg cgcggcagcg gtaggcccgt cggctcgctg acccagatgg
     1981 gcaccatccg cctcggcaag cgtacggaga atcgctcacc cctcatccgc gagttcgtcc
     2041 cgctggccaa cctcgacgac atcgtcttcg gcgcctggga ccccttcccc gacaacgcct
     2101 acgaagcagc gctgaagtgc ggcgtcctcg accgccacga acacatcgag ccgatcgccg
     2161 acttcctcaa atccatccac cccatgccgg ccgccttcga ccagtactac gtcaagcgcc
     2221 tcgacggacc caacgtcaag cgcggcacga agatggagat ggccgagcag atccgcgcgg
     2281 acattcgcaa cttcaagtcc gcgaacgcgt gcgaccgcct ggtcatgatc tggtgcgcct
     2341 cgaccgagat cttcatggag gaaggacgca gccacggcaa cctcgacagc ttcgaggcgg
     2401 cgctcgaggt cgacgacgag acgatcgccc cctcgatgat ctatgcctat gccgccctga
     2461 tggagggggt ccccttcgcc aacggcgccc ccaacctgac ggccgatatc ccggcgctgg
     2521 ctctcctcgc gaacgagcta ggcgtgccga tctgcggcaa ggacttcaag accggccaga
     2581 ccatgatcaa gacggtgctg gcgccgatgc tgaaggcacg catgctcggc ctcagtggct
     2641 ggtactcgac caatatcctc ggcaaccgcg acggagaggt gctcgacgac cccgagagct
     2701 tcaagaccaa ggaagagtcg aagctcggcg tcctcgagta catcctccag ccccagctct
     2761 accccgaact ctatagcgac gtctatcaca aggtgcggat caactactac ccgccgcgcg
     2821 gcgacaacaa ag
//
LOCUS       DASUBI010000641         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_891767, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000641 DASUBI010000000
VERSION     DASUBI010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891767"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..869
                     /locus_tag="VFU72_12325"
     CDS             54..869
                     /locus_tag="VFU72_12325"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5424322.1"
                     /translation="MKIYISVDIEGVAGVVDRSHGSLTGGAEYDLGRRLMTLETNAAI
                     EGALEAGATEIVVNDSHGPMINLLPEMLHPKATLIQGRLKHLFMVEGLDDSFDGMFLV
                     GYHASAGIRDGVLNHAFHPYELRYNGTVWSETGLNASVAGYYGVPVALVTGDDAAIRD
                     AETILPPHVGVSVKRGISRLAGESLSPSEARQRIREGAQQAIAQLSEMKPLVVQTPVT
                     IEMDFYYSIQADVAALIPTMERTGDRTIRFQAADAVSAYRAFLSTYYITRGLV"
     gene            1324..2697
                     /locus_tag="VFU72_12330"
     CDS             1324..2697
                     /locus_tag="VFU72_12330"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5424323.1"
                     /translation="MSTEWLRPGETRATRILILGGGFAGVYAGFELQKACAGQHVEIS
                     IVNRENFYVFYPLLPEIISGAVEVENVLNPIRLVVPKANLYVGDVTAIDVEQARVEIR
                     HGLYRHKQEERTLFYDHLIVALGGVPATSRIPGLAENAFDVQRLSSAFALRNHLIDTL
                     EQADIETDPLRKRRILSYVVVGGGTNGVEVAGEMHALLHEACRFYPQIEPEDIRVVIV
                     QSADRLFPDLPKQLADFAARLLRQRGVEIIFGRRVARVEPGVVYLDDGQTIEADTIVG
                     SVGVETNPLVNELPLPHDPRGRLAVQNTLRTGDEHPNVWAIGDAARVIDPHTGQPYPQ
                     TAQHAVREARLVARNIAAALRGEQPEPMTYRTLGQLVALGHRSAVAYIPPIKLSGFTA
                     WWIWRTYYLLQIPRWEKRLRVMFDWTLDLVFPPELVQLKVGQPVPSAGRSLAGLLGQR
                     AKPDGEG"
     gene            complement(2798..>3613)
                     /locus_tag="VFU72_12335"
     CDS             complement(2798..>3613)
                     /locus_tag="VFU72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5424324.1"
                     /translation="GPRRTHENEAFRQAGVYDLGGEVSYAAPELTPEEISADPVETLG
                     RMGGQIADAVAAGIAAGRRVVVAGGNCTALPGVIGGLQQALGPDARLGLVWFDAHGDF
                     NTPRTTHSGMLGGMPVAVSAGLCHANWRIGAKQPVPLPTDRIIMVDVRNLDPEEGDLV
                     RATDVTVVPAERGALADAVRRLADETDFIYLHIDLDVLDESLTPSHGTKEPNGPGMQE
                     TLEAVEAVLETGKVGAFGIVSIYPVGPGGDVSLASGTELLGESLKRWQAVGEG"
BASE COUNT          578 a         1289 c         1188 g          558 t
ORIGIN      
        1 ttcctcccct acgattaatc gacgaatcgg catttacgat gaagagggct tcgatgaaaa
       61 tctacatatc agtcgatatc gaaggtgtcg cgggggtcgt cgatcggtcg cacgggtcgc
      121 tgaccggcgg tgccgaatac gatctcggtc gccggctgat gacgctggag acgaacgcgg
      181 cgattgaggg ggcgctggag gcgggcgcta ccgagattgt cgtcaacgac agccacggcc
      241 cgatgatcaa cctcttgccg gagatgctcc atccgaaggc gacgctcatt caggggcgtc
      301 tgaagcatct gttcatggtt gaggggctgg acgactcgtt cgacggcatg ttcctcgttg
      361 gctaccacgc ctcggccggc atccgcgacg gcgtgctcaa tcatgccttc cacccatacg
      421 aactgcgcta caacgggacg gtttggagcg agacgggcct gaacgcttcg gtggccgggt
      481 actacggcgt tcccgtggca ctggtgaccg gtgatgacgc cgccatccgc gatgccgaaa
      541 cgatcctgcc gccgcatgtc ggcgtctcgg tcaagcgcgg catctcgcgc ctggccggcg
      601 aaagcctctc gcccagcgag gcacgccagc gcatccgcga gggcgcccag caagccatcg
      661 cgcagttgtc cgagatgaag ccgctggtgg tgcagacgcc ggtcacgatc gagatggatt
      721 tctactacag catccaggcc gacgtggccg cactgatccc gaccatggag cgcactggcg
      781 accgcaccat ccgcttccag gcggcggacg ccgtctccgc ctaccgcgcc ttcctctcga
      841 cctactacat cacgcgtggg ctggtctagc ccgcgggttt gctcgacagg catacttgag
      901 ccgtacgcct tcaggccggc tgcccccgcg gcagccggcg ccggacggcg acacgaacga
      961 acgagcgagt ttcttggaga gaaatctatg accggagcga cctgggttct gttggttctg
     1021 atcgtgctga tggccctcac cgcctggatg ggcagccacg ccacctaggg cgagcttctc
     1081 ggcaaacgac aaggctcggc gccactccag gacggatcct tcgactgcgc gcaggatgac
     1141 cgggagaggg agcagcctat cggacgacac tcctcgtcgt cgtccgcccc aaattaaccc
     1201 tgtcatcccg agcgcagtcg agggatccgt cctcactcgg tgccaatgct cctcgacgag
     1261 cagcggcccg cggagcgtcc cgtagacaca ctatccacca gcacacggga agggaggcac
     1321 gccatgagca cggaatggct acggcccggt gaaacgcgcg ccacgcgcat cctgatcctc
     1381 ggcggcgggt tcgccggggt ctacgccggc ttcgagctgc agaaggcctg cgccggtcag
     1441 cacgtcgaga tcagcatcgt caaccgcgag aacttctacg tcttctaccc gctcttgccc
     1501 gagatcatct ccggcgcggt cgaggtcgag aacgtactca accccatccg cctggtggtg
     1561 cccaaggcca acctctacgt cggcgacgtc accgccatcg acgtggagca ggcgcgcgtc
     1621 gagatccggc acggcctcta ccgccacaag caggaggagc gcaccctctt ctacgatcac
     1681 ctgatcgtgg cgctgggcgg cgtccccgcc acctcgcgta tccccgggct ggccgagaat
     1741 gccttcgacg tgcagcgcct cagttccgcc ttcgcgctgc gcaaccacct gatcgacacg
     1801 ctggagcagg ccgacatcga gaccgatccg ctgcgcaagc ggcgcatcct gagctacgtg
     1861 gtcgtcggcg gcggcaccaa cggggttgag gtggccggcg agatgcacgc gctgctgcac
     1921 gaggcctgcc gcttctatcc gcagatcgag ccggaggaca tccgcgtcgt catcgtgcag
     1981 agcgcggatc gactcttccc ggacctgccc aagcaattgg cggacttcgc cgcgcgcctc
     2041 ttgcgccagc gcggggtcga gatcatcttc ggccgccggg tcgcgcgcgt cgagccgggc
     2101 gtggtctacc tcgacgacgg ccagaccatc gaggccgaca ccatcgtcgg ctcggttggg
     2161 gtcgagacca acccgctggt gaacgagcta cccctgccgc acgacccgcg cgggcgcctg
     2221 gcggtgcaga atacgctgcg caccggcgac gagcacccca acgtctgggc catcggcgac
     2281 gccgcgcggg tcatcgatcc gcataccggc cagccctacc cgcagaccgc gcagcacgcc
     2341 gtgcgcgagg cgcggctggt ggcgcgcaac atcgccgccg cgctgcgcgg cgagcaaccc
     2401 gagccgatga cctaccgcac gctggggcaa ctggtggcgc tggggcaccg ctcggcggtg
     2461 gcctacatcc cgcccatcaa gctctccggc ttcaccgcct ggtggatctg gcgcacctac
     2521 tacctgctcc agattccgcg ctgggagaag cgcctgcggg tgatgttcga ctggacgctg
     2581 gacctggtct tcccacccga gctggtgcag ctcaaagtcg gccagccggt ccccagcgcc
     2641 ggccgcagcc tggccggcct gctcggccag cgcgccaagc cggatgggga ggggtagagg
     2701 cgccgacgcg agagtgtggc agccgacgac cgcgcggcct aaagccaccg cgcgtccgac
     2761 aggcgccgcg gctcggggaa gacgcaaacc ctcgccgcta cccctctccc accgcctgcc
     2821 agcgcttgag gctctcgccc agcagctcgg tgcccgaggc cagcgagacg tcgccgccgg
     2881 ggccgaccgg gtagatcgag acgatgccga aggcgcccac cttgcccgtc tccagcacgg
     2941 cttcgaccgc ctccagcgtc tcctgcatcc ccggcccgtt cggctccttg gtgccgtgtg
     3001 agggggtcag cgactcgtcg agcacgtcga ggtcgatgtg caggtagatg aagtcggtct
     3061 cgtcggccag ccgccgtacg gcatcggcca gcgccccgcg ctcggccggc acgacggtca
     3121 cgtcggtggc gcgcaccaga tcgccctcct cggggtccag gttgcgcacg tcgaccatga
     3181 tgatgcggtc ggtcggcagc ggcaccggct gcttggcgcc gatgcgccag ttggcgtggc
     3241 agagtccggc gctgacggcg accggcatgc cgccgagcat accgctgtgg gtggtgcgcg
     3301 gggtgttgaa gtcgccgtgc gcgtcgaacc agaccaggcc gaggcgggcg tccggcccga
     3361 gcgcctgctg caggccgccg attacgcccg gcagcgcggt gcaattgccc ccggccacga
     3421 ccaccctgcg cccggccgcg atccccgcgg ccaccgcgtc ggcgatctgg ccacccatgc
     3481 ggcccagcgt ctcgaccggg tcggcgctga tctcctccgg cgtcagctcg ggcgcggcgt
     3541 agctgacctc gccgcccagg tcgtagaccc ccgcctggcg aaaggcctcg ttctcgtgcg
     3601 tccgccgcgg ccc
//
LOCUS       DASUBI010000642         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_352350, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000642 DASUBI010000000
VERSION     DASUBI010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352350"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFU72_12340"
     CDS             <1..954
                     /locus_tag="VFU72_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5424325.1"
                     /translation="LSYTLSSAPAASGQEIEGARQALAQLTEAFQRAQKENAALRGSV
                     QAQSQQVADAQTTQQVADQQRQVEVAAAGSQVQAQVSQWQGQVATAAQLTQDRLAQLD
                     SSIHATEQLDAALRAKLGMPAIGYATTTIPDLKTVVDPQGAAQAALASLQARLDAVTA
                     DLNTVSGKADELARAQLLARTATTGQFVWPSGGTITQPFGPTSLTLEPAYEGYAHFHQ
                     GMDIAASMNTPIVAADGGTVVFAGWTDAGYGNCVQIDHGNGLITLYGHMAQTPLVHVG
                     QRVAKGQQIGLMGSTGNSTGPHLHFAVLKNGVWVNPANYLP"
     gene            complement(1054..2526)
                     /locus_tag="VFU72_12345"
     CDS             complement(1054..2526)
                     /locus_tag="VFU72_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424326.1"
                     /translation="MRTWAANHWSPAAWLQQQLQSRTRGEPSAKLFRGLRLRLTIWYG
                     LVLTASLLAVGVSLYLVLQHQLLETVGDQLSAQNAILAREWVRLPNRGCPGIATLGSR
                     SGSSMLPIYVACYDTGGGLLGVGTGDASRLTPPNAFLDGNLVQTALEDGSASDIVEGG
                     PQVGPIFRYAVAVHIPGSGEERSVIQVGQSVSQVEGALGQLRRLLLLFGFSSLALATI
                     GGLWLSNRALAPARLAFARQQAFIADASHELRTPLTLLRANAEVLLRHRQRFPEGDAE
                     LLEDIVGETAHMSIMAGNLLTLARLDAGRLIAERDILSLSELMRAAARRVSALAAEED
                     LSITLELAPDAAVVGDRAALEQAILIFIDNAVKYTPPGGRITLRTSDDDGVARAAVSD
                     TGIGIAPDYLPRLSERFYRVDVARSRATGGAGLGLAIAHGIAAAHQGRIEIDSEPNSG
                     TTVTLSLPAARRRARTQIASLETNDDAGSPGKTDPSTSSG"
     gene            2774..3850
                     /locus_tag="VFU72_12350"
     CDS             2774..3850
                     /locus_tag="VFU72_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5424327.1"
                     /translation="MPEAIAELIQDIEEAMALLEDRLSGHAERIESFVDELEQQRAVV
                     QSAAEEHQLEAMLAGQESDEDDDILRELRRREDQLRDQYTNVSSLYRQVSGFAHLLTA
                     SRQRFDDGGELPGIEDGQRLAVREAIIRAQEDERRRLAREIHDGPAQVLANAIIGLEF
                     VDRSLQQATLASGGERLTAELERVKAAMREGLIEIRRFIFDLKPSMLNQRGLAPTVEH
                     YIQSYRHLFPGDIHLEIPENLPHLTPEQEMTAFRVIQESLQNVHKHSRSTQTWVIISS
                     EADGLLVEVRDNGQGFRPSTTGPSLSGGFGLVGMRERAEVLGARLSVESEPGTGTSIR
                     LVIPLNRRAMRPARERDLRRKLKI"
     gene            3859..>4187
                     /locus_tag="VFU72_12355"
     CDS             3859..>4187
                     /locus_tag="VFU72_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424328.1"
                     /translation="MNRLQILVIDDHPLFRRGIRWSLEEQRDLVVVGEASDGQEGVRL
                     SDVLVPDVVLLDLNLPGMSGLEVARVLKRRHPQLGIIVLTMHEDDEQLFSAIRAGANA
                     YSTKDVDP"
BASE COUNT          693 a         1388 c         1480 g          626 t
ORIGIN      
        1 ctcagctaca cactgagcag cgctccggcg gcctcagggc aggagatcga gggcgcgcgc
       61 caggcgctgg cgcagttgac cgaagccttc cagcgggctc agaaggagaa cgccgcgctg
      121 cgcggctcgg tgcaggcgca gtcgcagcag gtcgcggacg cgcagacgac gcagcaggtg
      181 gcggaccagc agcggcaggt cgaggtcgcc gccgccggca gccaggtgca ggcgcaggtg
      241 agccagtggc agggccaggt cgccacggcc gcgcagctga cccaggaccg gctggcgcag
      301 ctcgacagct ctatccacgc gaccgagcag ctcgacgcgg cgctgcgggc caagctcggc
      361 atgccggcga tcggctacgc caccaccacc atccccgacc tgaagacggt cgtcgatccc
      421 cagggcgccg cgcaggccgc gctggcctcg ctccaggcgc ggctcgacgc ggtcaccgcc
      481 gacctcaaca ccgtgagcgg caaggcggac gagctggcgc gggcgcagtt gctggcccgc
      541 accgccacga ccgggcagtt cgtctggccg agcggcggga cgatcaccca gcccttcggg
      601 ccgaccagtc tcacgttgga gccggcctac gagggttacg cccactttca ccagggcatg
      661 gatatcgccg ccagcatgaa taccccgatc gtggcggccg acggcggcac cgtcgtcttc
      721 gccggatgga ccgacgccgg ctacggcaac tgcgtgcaga tcgaccacgg caacgggctg
      781 atcacgctct acggtcacat ggcgcagacg ccgctggtgc acgtcggcca gcgggtggcc
      841 aaggggcagc agatcgggct gatgggctcg accggcaact cgaccggtcc gcacctgcac
      901 ttcgccgtgc tcaagaacgg ggtctgggtc aacccggcga actacctgcc gtaacgcccg
      961 cacccgcacg gagaccggcc ccgctccgta cggagagagg ccctgcccgt cgacgacgct
     1021 tgctgccgcg tcaggacgga tccgtcaccc ctgtcatcca gagcttgtcg aaggatccgt
     1081 cttgcccggc gatccagcgt cgtcgttggt ctccagactg gcaatctggg tcctggcccg
     1141 tcgccgggcg gcggggaggc tgagcgtgac ggtcgtgccc gaatttggct cgctgtcgat
     1201 ctcgatgcgg ccctggtggg cggcggcgat gccgtgggcg atcgccaatc ccagcccggc
     1261 gccgccggtg gcgcgcgagc gggccacgtc cacccggtag aagcgctcac tcaggcgcgg
     1321 caggtaatcg ggcgcgatcc cgatgccggt gtcgctgacc gcggcgcggg cgacgccgtc
     1381 gtcgtcgctg gtgcgtaacg tgatccggcc tccggggggc gtgtatttga cggcgttgtc
     1441 gatgaagatc aggatggcct gttccagcgc ggcgcgatcg ccgaccacgg cggcgtcggg
     1501 cgccagttcc agggtgatac tcagatcctc ctcggccgcc agggcactga cgcgccgggc
     1561 cgccgcgcgc attagctcgg agaggctgag gatgtcgcgc tcggcgatca ggcggccggc
     1621 gtcgaggcgc gccagcgtca gcaggttgcc ggccatgatg ctcatgtgcg cggtctcacc
     1681 gacgatatcc tccagcaatt cggcgtcgcc ctcggggaag cgctgacggt gccgcagcag
     1741 cacctcggcg ttggcgcgca gcagcgtcag cggggtgcgc agctcgtgcg acgcgtcggc
     1801 gatgaacgcc tgctgccgag cgaaggcgag ccgggccggc gccagcgccc ggttcgagag
     1861 ccagaggccg ccgatcgtcg ccagcgccag cgacgagaag ccgaacagca gcagcaagcg
     1921 gcggagctgc cccagcgcgc cctcgacctg cgacaccgat tgcccgacct ggatgaccga
     1981 acgctcctcg ccgctgccgg ggatgtggac cgccaccgcg tagcggaaga tcgggccgac
     2041 ctgcggcccg ccctccacga tgtccgaggc gctgccgtct tcgagcgcgg tctgcaccag
     2101 attgccgtca aggaaggcgt tgggtggggt caggcggctc gcgtcgcccg taccaacgcc
     2161 gagcagtccg ccgccggtgt cgtagcaggc gacgtagatg gggagcatcg aggagccgga
     2221 acggctcccc aaagtggcga tcccggggca accgcggttg ggcagccgga cccactcgcg
     2281 cgccaggatg gcgttttgcg cgctgagctg gtcccccacc gtctccagga gctggtgctg
     2341 gagtaccaag tacaggctaa cacccacggc taagagcgag gcggttagga ccaggccgta
     2401 ccaaatggtc aaccggagac gcagcccgcg gaagagcttc gcgctgggct cgccgcgggt
     2461 ccggctctgg agctgctgct gcaaccaggc ggccggcgac cagtggttcg ccgcccaggt
     2521 cctcatcccg ccgtctccct gccgtccctg ggccgcaaat agcccattga tcgcctcacg
     2581 tcttactgtg acaatagagc aactacccag acccaggacc gttatgctat gtgaaagggg
     2641 ggtgtacgga cacccctggt gctatactat tgttattact tgtgattgtg ctgcgcccag
     2701 tactccgccg caagaggctg gccaggggca cgagagcgag tttgtcagga tgggggatga
     2761 cgtgccaggc gagatgcctg aagccatcgc ggagttgatc caggatatcg aagaggccat
     2821 ggcgctgctc gaggaccggc tctccggcca cgccgagcgg atcgagtcgt tcgtcgatga
     2881 actcgagcag cagcgcgcgg tggttcagag cgcggccgag gagcatcagt tggaggcgat
     2941 gctggccggt caggagtccg acgaagatga cgacatcctg cgcgagttgc ggcgccgcga
     3001 ggatcaactg cgggaccagt acaccaatgt ctcctcgctc taccggcagg tcagcggctt
     3061 cgcccacttg ctgacggcca gccggcagcg gttcgacgac ggcggggagt tgcctggcat
     3121 tgaggatggg caacggctgg ccgtacgcga ggcaatcatt cgcgctcagg aggacgagcg
     3181 gcgccggctg gcgcgtgaga tccacgacgg tccggcgcag gtgctggcca acgcgatcat
     3241 cgggctggag ttcgtcgatc gctcgctgca acaggcgacg ctggccagcg ggggcgagcg
     3301 cctgacggct gagctggagc gcgtcaaggc ggcgatgcgc gaggggctga tcgagatccg
     3361 gcgcttcatc ttcgacctca agccgtcgat gctgaaccag cgcgggctgg ctccgacggt
     3421 ggagcactac atccaatcgt atcgccacct cttccccggc gacatccacc tggagatccc
     3481 cgagaacttg ccgcatctga cgcccgagca ggagatgacc gcgttccgcg tgatccagga
     3541 atcgctccag aatgtgcaca agcattcgcg ctcgacgcag acctgggtga taatctcgag
     3601 cgaggcggac gggttgctgg tcgaggtgcg cgacaatggc cagggctttc ggccgagcac
     3661 gaccggcccc agtctgagtg gtggctttgg tctggtgggg atgcgcgagc gcgcggaggt
     3721 gctcggcgcg cgcttgagtg tcgagagtga accgggcacg ggaaccagca ttcggcttgt
     3781 tatcccgctc aaccggcgcg cgatgcgccc agcgcgtgag cgcgatctgc ggcgcaagct
     3841 gaagatttag gagggatcgt gaaccgtctg cagatcctgg tcatcgacga ccacccgctc
     3901 ttccggcgcg gcatccgctg gagtctggag gagcagcgcg atctggtcgt cgtcggcgag
     3961 gcgtcggacg gccaggaggg tgttcgcctc tccgatgtgc tggtgcccga cgtcgtcctg
     4021 ctcgacctta acctgcccgg catgagcggg cttgaggtgg cgcgcgtcct caagcggcgg
     4081 cacccgcagc tcggcatcat cgtcctgacg atgcacgagg acgacgagca gctcttcagc
     4141 gctatccgtg ccggagccaa tgcctattcg accaaggacg tcgaccc
//
LOCUS       DASUBI010000643         7294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_968247, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000643 DASUBI010000000
VERSION     DASUBI010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968247"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1181
                     /locus_tag="VFU72_12360"
     CDS             192..1181
                     /locus_tag="VFU72_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424329.1"
                     /translation="MFGLRVDSAVSLPIPALGDADDSRPDVRIVWEDRAAAPAGQPRA
                     ETRCYCPFHAGRVVMRVYREDKHSWIWHEGAGTSRVADDGRTITLTAGTDVIDERVRG
                     LILAGLGSMFVLHHRGVPCLHAAAVLLDGCAVAFLGAKGQGKSTLVSGLLRRQGTLLT
                     DDILPLQHEPEGISGLPSLPIMKLWDGSVTHALGVTDDLPVLTQNLDKRLFTLDERFT
                     FATEPVRLRAIYLLDRYDPLTAGEPGITVEPVARREALMTLLAQTPLRPYLTPEELAG
                     LLPLYQSLLLQAPLRRLRYPSGFAYQDTVYARLRADLAAAPGESDQTERLAPR"
     gene            1195..2973
                     /locus_tag="VFU72_12365"
     CDS             1195..2973
                     /locus_tag="VFU72_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424330.1"
                     /translation="MGLVIRFLRDHKGLWPVWLPLLAISIAAPISSVIIPLVEKRLID
                     TVILGHRPDLLMSTAGLYVGLWLLSTCLLVVGGLLRTYLGERLALALRYRIFTHYESL
                     SVAFAHREHSGRTMSLFVNDVPNTTSLFSTTIVSGVGAVIALLGATVVMFRLRPQRAV
                     VAVGGPLLAAVVAAVVTRPLRPAVRKVQEILAELNERLQENLEGIREVVAFGREEPQA
                     QHVLSILWNLVRLRMRVTKIEMTISTGASILSLAVTVTIFIYGTYLVLETRTSLGTVI
                     AMRSLFGLVFQPAGQLIGLFTSTQKALGAADRIYAFLDQTPRVVDKGERSLPQVAGEV
                     LFDAVDFSYREGEPVLEGVSFVAHPGQLTALVGPSGAGKSTVAALMARFYDPDHGRIL
                     LDDVDLRDLPLDDLRANVGFVFQDTFLFAASIRENIAFGREHASEEEVIAAAQAAQAW
                     EFIERLPEGLATLVGERGVQLSEGQRQRLAIARAFLRDPRVLILDEPTSALDARSEQL
                     LQAAMNNLTHGRTTFVIAHRLSTIQRADQILVFDGGRLVEHGTHVDLLDRHGLYRELC
                     ELQFGGATPAPQMLPGGSPLPAAVAD"
     gene            3101..3526
                     /locus_tag="VFU72_12370"
     CDS             3101..3526
                     /locus_tag="VFU72_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424331.1"
                     /translation="MGVGDLLRLKAILGVHAAIVIFALASLAWLYLGRRVRGADGALW
                     LAVPLLLDVAVICIAGPGHLYSKRYEGSILVELARHSGITIFDVTGLALATLAAALGG
                     FLLWRCWPQLQWWGELTPAGDSPAADAAAMNEPRTPASR"
     gene            complement(3568..4047)
                     /locus_tag="VFU72_12375"
     CDS             complement(3568..4047)
                     /locus_tag="VFU72_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424332.1"
                     /translation="MRRPRLPRLNPFHRNGLAKLDPEERENIERSALLEQVHSGLTAV
                     SGAESDLARQAQELRTALSLLAGVADRTAHAGLLSEANAVSQREAALTAALAEIEQRR
                     ELMQDIERQLRDAEERLAAEPARRVAEPPFPNDTVVEARLAGAGGPLLGSITGKSTD"
     gene            complement(4118..4801)
                     /locus_tag="VFU72_12380"
     CDS             complement(4118..4801)
                     /locus_tag="VFU72_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424333.1"
                     /translation="MLLIDDDTDLLDVTAYALRREGFNVMVATDGRAGLERWSRDEPD
                     VVVSDVIMPHLNGLQLCHEIRQSASTPVILLTAANSEEQIIQGFQMGADDYIVKPFSP
                     RQLAMRIRAVCRRGASAGMPEPTLQLQVGEFLLDVESHEVAYQGEQVQLTPIEFKLLH
                     ILAVNRGRVVNATRLVDYAWGYDGGDVFLLKTHISHIRKKLSLPRGGIEDIRSVPRVG
                     YRLTMNVPA"
     gene            complement(4804..5724)
                     /locus_tag="VFU72_12385"
     CDS             complement(4804..5724)
                     /locus_tag="VFU72_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5424334.1"
                     /translation="MVLHQRHVESPRRRHACLHENPDTRNETDTVVSSDWTPPSSAAL
                     RHRRTADDPARAPSQLPRPDLTSEIIGRLGHELRTPLTTIQALAELLADPELPAEERA
                     TLLEQHARSVGWMTSLLEDFSAWAAAQDGRLSLRCREARVLDWVERAVTLTQPFLSRR
                     AQRVQITCSNSALRIYGDPERLSQVLVNLITNAGRYSVWGDVIALHIQELNRDEIRLS
                     VTDHGPGIQPDIQARLFERYERGPAAGQAPHGQGLGLYIVRTLVELHGGRVGIESDPG
                     AGSTFWIVLTHWPHRDSVTACEPNERVGAE"
     gene            complement(6095..6487)
                     /locus_tag="VFU72_12390"
     CDS             complement(6095..6487)
                     /locus_tag="VFU72_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424335.1"
                     /translation="MRRYLPFAIGLLLPLGAAALVVWLVPATGPLSRLAQESLLAGTF
                     AVGLLGGLLLRRWWAVLAVPAIYAVAVWGLRLGTCTACGGPSSGIGLARALGYALLGL
                     APLVLGALIGIWSRAVARHQTIAEKAGD"
     gene            6707..>7294
                     /locus_tag="VFU72_12395"
     CDS             6707..>7294
                     /locus_tag="VFU72_12395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5424336.1"
                     /translation="MQRSVQLKNPAGSGQTLRYRLMKRVLDLLVATLVLLCLSPLFLV
                     CALLVRRSSAGPVLFRQRRVGQHGREFTCLKFRSMYSDADPAPHQAYVAAMIKGEAAG
                     YGEGDERLYKLVDDPRVTPIGRWLRRTSLDELPQLWNVLRGEMSLVGPRPPIPYELEN
                     YDARHLARLAVKPGITGLWQVSGRSRTSFEDMVRLD"
BASE COUNT         1154 a         2449 c         2418 g         1273 t
ORIGIN      
        1 ctgagcgggc aggtggtcaa cgaccacccc gccgcgatcg gcagcttcgc gcccttgatt
       61 cggcacggcg ctggcgatct cctggcgcta ccgacgatcc ggctggatgc ccggaaagtg
      121 cggtggctgt gaacacggca acacccctcc cccaggcaag cgcatcgacc gctgcgacgt
      181 atcgctaccg cttgttcggc ttgcgcgtcg atagtgccgt ctcgctgccg atcccggcat
      241 taggcgacgc cgatgacagc cggccggatg tccgcatcgt ttgggaagat cgcgcagcgg
      301 caccagcagg ccaaccccgc gcggagacgc gctgctactg cccgttccac gccgggcgcg
      361 tcgtcatgcg tgtctaccgc gaggacaaac acagttggat ctggcacgag ggcgccggca
      421 ccagcagagt ggcggacgac gggcgcacga tcacgcttac cgccggcacg gacgtaatcg
      481 atgagcgcgt gcggggattg atcctggccg ggctggggtc gatgttcgtg ctgcatcatc
      541 gcggcgtccc ctgcctgcac gctgccgctg tgctcctcga cgggtgcgcc gtggcgtttc
      601 ttggcgccaa gggccagggc aaatcgaccc tcgtctcagg gctgttgcgc cgccagggga
      661 cgctcctgac tgacgacatc ctgccgctcc agcacgaacc ggagggcatc agcgggctgc
      721 cgagcctgcc gatcatgaag ctgtgggacg ggagcgtgac gcatgcattg ggcgtgacgg
      781 atgacttgcc cgtgctcacg cagaatctgg acaaacggct cttcacgctc gacgagcgtt
      841 tcacgttcgc cacggagccg gttcgcctgc gcgcgatcta cctgctcgat cgctatgatc
      901 cgctgaccgc cggggagccg ggcataacgg tcgaaccggt cgccaggcgt gaggcgctca
      961 tgacgctgct agctcagacc ccactgcggc cctacctgac gcccgaagag ctggctgggc
     1021 tgctgccgct ctaccagtca ctcctcctcc aggctcccct gcgtcgcctg cgctacccca
     1081 gcgggtttgc ctaccaggac accgtttacg cgcgtctcag ggccgatctt gcggccgcgc
     1141 caggggaatc tgaccagacc gagcgactcg cgccgcgatg aaggtgagag accggtgggg
     1201 ctagtcattc gcttcctgcg cgatcacaag ggactgtggc cggtttggct accgctgctg
     1261 gcgatctcga tcgcggcccc gatcagttcg gtcatcatcc cgctcgtcga gaagcggctc
     1321 atcgacacgg tgattctggg gcatcgtcct gatctcctca tgagcacggc cggactctat
     1381 gttggtctct ggctcctcag cacctgtctg ctcgtcgtcg gcggcctgtt gcgtacctac
     1441 ctgggagagc gcctggcgct ggctctgcgc tatcgcatct tcactcatta cgagtcgctg
     1501 tcggttgcct tcgcgcaccg ggagcactcc ggccgcacga tgtcgctctt cgtcaacgac
     1561 gtaccgaaca cgaccagcct cttcagcacc acgatcgtca gcggggtcgg cgcggtgatc
     1621 gcgctgctgg gcgccaccgt cgtcatgttc cgcctccgcc cgcagcgggc ggtggtcgcc
     1681 gtcggcgggc cgctgctggc ggcggtcgtg gccgcggtcg tgacgcgccc gctccggccc
     1741 gccgtccgca aagtgcagga gatactcgcc gagctgaacg agcgcttgca ggagaacctg
     1801 gaggggatcc gggaagtcgt cgccttcggc cgggaagagc cgcaggcgca gcacgtgctc
     1861 tcgatcctct ggaacctcgt ccgactgcgt atgcgcgtca ccaagatcga gatgaccatc
     1921 agcaccggcg cgagtattct ctcgctggcg gtaactgtga ccattttcat ctatggcacg
     1981 tacctggtgc tggaaacgcg aacctcgctg ggcacggtca tcgccatgcg ctcgttgttc
     2041 gggctcgtct tccaaccggc gggccagtta atcggcctct tcaccagcac gcagaaggcg
     2101 ctgggcgcgg ccgaccgcat ctatgcgttc ctggaccaga cgccgcgagt ggtcgacaag
     2161 ggagagcgtt cgctgcccca ggtcgccggc gaagtcctgt tcgacgcggt cgacttctcc
     2221 taccgggagg gcgagccggt gctcgaagga gtctccttcg tggcgcaccc gggccaactg
     2281 accgcgctgg tggggccgag tggggccggc aagagcacgg tggctgcgct gatggcgcgc
     2341 ttctacgatc ccgatcatgg gcgcattctg ctcgacgatg tcgatctgcg cgacctcccg
     2401 ctcgatgatc tgcgcgccaa cgtcggcttc gtcttccagg acacgttcct cttcgccgcc
     2461 agcatccgcg agaatatcgc gttcggccgt gaacatgcct cagaggagga ggtcatcgcg
     2521 gcagcgcagg cggcgcaggc ctgggaattc atcgagcggc tgccggaggg gctggccacg
     2581 ctggtgggcg agcgcggggt gcagctttcc gagggccagc gccagcggct ggccatcgcg
     2641 cgggcgttcc tgcgtgaccc ccgtgttctg atcctcgacg agcccacctc cgccctcgat
     2701 gcgcgctccg agcaactgct gcaggccgcc atgaacaacc tgacgcacgg ccgcaccacc
     2761 ttcgtcatcg cacaccggct cagcacgatc cagcgcgccg accagattct ggtcttcgac
     2821 ggcggccgtc tggtcgagca cggcacgcat gtcgacctgc tcgaccggca tgggctctat
     2881 cgtgagctgt gcgagctgca attcggcggc gcgacgcctg ctccccaaat gttgccgggc
     2941 ggctcgccgc tgccggccgc ggtggcggac tgaccctcac cggagaccca gccggtcatg
     3001 gcgaagaatg ggtataactg accgtcagca gccgggcggc tgggtaggcg acgccgagct
     3061 ccccggtgac cgcggtacag cgaagctgga agggtgaccc gtgggcgtcg gtgatctgct
     3121 ccgtctcaag gctatactcg gagtacacgc cgcgatcgtg atctttgccc tggcgagcct
     3181 tgcctggctc tatctcgggc gacgagtgcg cggcgcggac ggggcgctct ggttggccgt
     3241 gccgctcttg ctcgatgtgg cggtcatctg catcgccggc cccggtcacc tctactccaa
     3301 acggtatgaa ggatcgattc tggttgagct ggcacgtcac tccggcatca cgatcttcga
     3361 tgtcaccggg ttggccctgg ctacgttggc ggccgcgctc ggcggcttct tgctctggcg
     3421 ctgctggccg cagcttcagt ggtggggtga gctgacgccg gccggcgaca gcccggctgc
     3481 cgatgcggcc gccatgaacg agccgaggac tccagcttcg cgttgatgct gcgtgggaag
     3541 ccgcgcggcc tgtcgtccgc ggcgcgatta gtccgtggac ttcccggtga tgctgccgag
     3601 cagtggtccg ccggcgccgg ccaggcgggc ctcgacaacg gtgtcgttgg ggaacggggg
     3661 ttccgcgacg cgccgcgccg gttcggcggc cagtcgctcc tcggcatcac gcagttgtcg
     3721 ctcgatgtcc tgcatcagtt cccgtcgctg ctcgatctcg gcgagcgcgg cggtgagggc
     3781 cgcctcacgc tgggagacgg cgttggcctc ggacagcaga ccggcgtgcg cggtgcggtc
     3841 ggccacgccc gccagcaggc tgagtgcggt gcgcagctcc tgcgcctgac gtgccagatc
     3901 gctctccgca ccggaaaccg cggtcaggcc gctgtggacc tgctccagca gggctgagcg
     3961 ttcgatgttc tcccgctctt cggggtccag tttggccagc ccatttcgat ggaagggatt
     4021 cagccgtggc agtcgtggtc tccgcatggg cctcctcgtt ccgtgcgcgg tggacgggtg
     4081 acaacgttga ctgactggac gcaatatccg ttcccgttca ggcgggtacg ttcatagtca
     4141 ggcgatagcc gacgcgcggc acggagcgaa tatcctcgat gccaccgcgg ggcaaactca
     4201 gcttcttgcg aatgtggctg atgtgggtct tcagcagaaa gacgtcgcct ccgtcgtagc
     4261 cccaggcgta gtccaccagg cgggtggcgt tgaccacccg gccgcggttg accgccagaa
     4321 tatgaagcag cttgaactcg atcggcgtca actgcacctg ctccccctgg taggcgactt
     4381 cgtgcgactc gacatcgagc aggaactcac ccacctgcag ctgaagggtc ggctcgggca
     4441 tgccggctga tgcgccccgg cggcagaccg cgcgaatgcg catcgccaac tgccgcggac
     4501 tgaacggctt gacgatgtag tcgtcggcgc ccatctggaa cccctggatg atctgctcct
     4561 cgctgttggc agcggtcagg agaatcaccg gggtggaagc ggactgccga atctcgtggc
     4621 agagctgcag gccgttgagg tgcggcatga tgacatcgct gacgacgaca tccggctcat
     4681 cccgcgacca gcgctccagc ccggcgcgac catcggtggc gaccatgacg ttgaaacctt
     4741 cgcgccggag cgcataggcg gtcacgtcca gcaggtcggt gtcgtcgtca atcaggagca
     4801 ctttcactct gcaccgaccc tttcattcgg ctcgcacgct gtgacgctgt cgcgatgcgg
     4861 ccaatgcgtg aggacaatcc agaacgtgct gccggccccc gggtcactct cgatgccgac
     4921 ccggccaccg tggagctcga ccagagtacg cacgatgtag aggccaagcc cctggccgtg
     4981 cggcgcctgc cccgcggccg ggccgcgctc gtagcgctcg aacaatctcg cctggatgtc
     5041 cggctgaatg cccggcccat ggtcggtgac cgagagccgg atctcgtcgc ggttgagctc
     5101 ctggatgtgc agagcgatca cgtcacccca gacgctgtag cggccggcgt tggtgatcaa
     5161 gttgacgagc acctgactca gccgctcggg atcaccgtag atgcgcagcg cggagttgga
     5221 gcaggtgatc tgaacccgct gtgcccggcg cgagaggaat ggctgggtca gggtgaccgc
     5281 ccgctcgacc cagtcgagca cgcgcgcctc gcggcagcgc agcgacaagc ggccatcctg
     5341 cgccgcggcc cacgcgctga agtcttccag caagctcgtc atccacccga cactgcgcgc
     5401 gtgctgctcc agcagcgtgg ctcgttcctc ggcgggcaat tcgggatccg ccaggagctc
     5461 ggccagagct tggatcgtgg tgagcggggt gcgcagctcg tgaccgagcc gcccgatgat
     5521 ctcgctcgtg agatcggggc gcggcagctg agacggcgcg cgcgcggggt catcggccgt
     5581 gcgacgatgg cgcagcgcgg ccgatgacgg cggcgtccag tcggacgaaa cgacggtatc
     5641 ggtctcgttg cgtgtgtccg gattctcgtg aagacaggcg tggcggcggc gtgggctttc
     5701 gacgtggcgc tgatgcagga ccataccgat ccctctcctc ttccctcgaa ccaggaccac
     5761 ccaacccaca cgacgcggag attgagccgt caagctatat aacgtagagc taaatcgtcc
     5821 ggttgcccaa taggactgtg accatcagga caggggcaat tgctcgtgtt gctgcgcggc
     5881 gtagcgccga tgcctccctc acctcgcgtc cccggtacgc gcaaacgcaa aatcggtacc
     5941 acgtttcgtg tgtcgtgtct cgtgaatcta gcgttgctga agctattaga agtactcacg
     6001 aatatagtgt caagtgctcc ggtgtgtctt atggtcaagc accggttgag aagtggtcga
     6061 gaaccggtgg cccatcccgc gaggcggctg ccggctagtc gccagccttc tcggcgatgg
     6121 tctggtggcg tgcgacggcg cggctccaaa tgccgatcaa cgcgcccagc accagggggg
     6181 ccaatcccag cagcgcgtag cccagggcgc gggccaggcc gatcccgctc gatggaccgc
     6241 cgcaggcggt gcaggttccc aaacgcagcc cccagacggc cacggcgtag atggccggca
     6301 cggcgaggac cgcccaccag cgcctgagca ggagtccgcc gagcagccca accgcgaacg
     6361 tgcccgccag cagcgactct tgcgccagcc ggctcaaggg gccggtagca ggaaccagcc
     6421 acaccacgag cgcggctgcc cccaagggga gtaggagccc gatcgcaaac ggcagatagc
     6481 gtctcacgcc cgatgtcctc agcactgacg atcgcctctc gacacgcgtt gctctagcat
     6541 acgagccgct gcaagccggg cgcggtcagc gcagccggcg cacactcgcc cttcgctacg
     6601 cgacattcct caaccaatgc tttacgggcg gcatacccgc cagtaacgtc ctgccgctac
     6661 gctgcaatct gtaaaccccg cgctaccgat tctgtcggag gcgacgatgc agcgttcggt
     6721 gcaactcaag aacccggccg gcagcgggca gacgctgcgg tatcgcctga tgaagcgcgt
     6781 gctcgacctg ctcgtggcca cgctggtgtt gctctgcctg tcgccgctct tcctcgtctg
     6841 cgcgttgctc gtgcggcgta gttcggcggg gccggtgctc tttcgccagc ggcgagtcgg
     6901 tcagcacggg cgcgagttca cctgtctcaa gtttcgttcc atgtattccg acgccgaccc
     6961 cgcgccgcac caggcctacg tggccgccat gatcaaaggg gaagccgccg gctacggaga
     7021 gggggacgaa cgcctctaca agctggtcga cgatccccga gtgacgccga tcggccgctg
     7081 gctccggcga accagcctcg acgagctgcc gcagctctgg aacgtgctgc gcggcgagat
     7141 gagtctggtt ggaccgcgac cccccatccc ctacgagttg gagaactacg acgcgcgtca
     7201 tctggcgcgc ctggctgtca agccggggat caccggcctg tggcaggtga gcggccgcag
     7261 ccgcaccagc ttcgaggaca tggtccggct cgat
//
LOCUS       DASUBI010000644         4922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_256878, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000644 DASUBI010000000
VERSION     DASUBI010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256878"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="purM"
                     /locus_tag="VFU72_12400"
     CDS             <1..744
                     /gene="purM"
                     /locus_tag="VFU72_12400"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5424337.1"
                     /translation="YYATPELDPEALAEIVAGMADACEAAGCALVGGETAQLPGIYRD
                     GVYDLAGFMVGGVAEDEIVDGSAIRPGDVLVGLPSSGLHTNGFSLARAALGLNGPPAE
                     LRPRLAQLSLWSDRSLGEILLTPHRSYLPVIEPLLGTGLIHGMAHITGGGLAGNIGRV
                     VPQDLQAVIELGSWQVPRLFLAIEDSGQIDEAEMYRVFNMGIGFVVILAANDIVRLTE
                     QEEAAVVIGHIVPSDAPERVTLERAGGAP"
     gene            741..1265
                     /locus_tag="VFU72_12405"
     CDS             741..1265
                     /locus_tag="VFU72_12405"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5424338.1"
                     /translation="MSGFDRSLSRLVITDEDRMQIVTCLQRLMTDSSASYCMVIDRSG
                     QVLAWESDQERPEMTYLGALIAATYASTQEMARILKEDGFKTLLQEGLKERVYTETIK
                     DRWLLVVIFDQQSQVGLVKVLARRESVVLAGVLDLSQERGDHPDAQRFRRMGKATTDT
                     IDLIFKDNGYSDDE"
     gene            1280..1855
                     /locus_tag="VFU72_12410"
     CDS             1280..1855
                     /locus_tag="VFU72_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HEU5424339.1"
                     /translation="MALINVAAREIHGKIVYYGPGLSGKTTNLQFIHSRIPNNTKGDL
                     LSIETETERTLFFDFLPLDLGKVHGFSIRFHLYTVPGQILYERTRVAVLNGADGVVFV
                     ADAGRDRLPDNLQSLRELAQNLTAQGKRFLDYPLVMQYNKMDLPEALPIPVLDRYLNT
                     IRVPRFEAVARDGKGVFETLRAISKLVVNKL"
     gene            1902..2528
                     /locus_tag="VFU72_12415"
     CDS             1902..2528
                     /locus_tag="VFU72_12415"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5424340.1"
                     /translation="MPNGAAAGERDPSLERAQSAAAIPTPEPEHSLHVAELSVQIFDQ
                     LADPLALPAGERDLLAAAALWHDAGQRFTLPEHHLRSFDIIVNQRLLGFDDEDRLAIA
                     NIARYHRQSTPELEHTGYRNLRRERRPLVDRMASILRLAEALDASHLQLVERVVATVS
                     PGLVEFDVVAASYPTIEVERAQARGGLFRRVFRRETSFRWHRQAPGSA"
     gene            2528..4060
                     /gene="purH"
                     /locus_tag="VFU72_12420"
     CDS             2528..4060
                     /gene="purH"
                     /locus_tag="VFU72_12420"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5424341.1"
                     /translation="MPRALLSVSDKSGLAVFARRLARLGFELISTGGTLQALTDAGVP
                     AISVVDVTGFPEIMAGRVKTLHPAIHAPLLARRDVPEDLAIMEEMGFTGIDLLAGNLY
                     PFAQTVARPGVTLADALENIDIGGPAMIRAAAKNFPAVLVVVDPADYDPLADAIERES
                     LTGIDLERRQALAAKAFAHVSAYDAVIAAYLRPEDQFPERITVAGEKLADLRYGENPH
                     QPGALYRRLAPGPVAGVGVWRLLAGPEMSFNNYLDANAALTAALGFPEPTCAIIKHAN
                     PCGIASDPDLGAAYEAALASDPVSAYGGIVALNRPLDIVAAERIAASHFDIVIAPDFV
                     EGALARLRRKKRLRLLAAPAPQVASEPEIRPLEGGFLLQEPDRLPVDPSAWQLVSRRA
                     PRDAEMDALVFAWRAVRYVKSNAIVLAQGEATVGIGGGQPNRVDAVRIAVARAGERAA
                     GSVMASDAYFPFPDNIEVAAAAGVRAVVAPGGSRRDPEVIAAADAANLTLLFTSARHF
                     RH"
     gene            4521..4874
                     /locus_tag="VFU72_12425"
     CDS             4521..4874
                     /locus_tag="VFU72_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424342.1"
                     /translation="MQTTVTLRQFKRRAQTTIIGLGLLALGFSGAVALGQTDLRHQHA
                     AAPVLAAPVSQGLSYDDFRYYDENTELPSSANAPAGIGDAAPAPITPDRMKFLEQNTQ
                     LPDTQAIPVQRGGPR"
BASE COUNT          817 a         1717 c         1562 g          826 t
ORIGIN      
        1 tactacgcga cacccgagct tgatcccgag gcgctggccg agatcgtcgc tggcatggcc
       61 gacgcctgcg aagccgccgg ttgcgcgctg gtcggaggcg agacggccca gctccccggc
      121 atctaccgcg atggtgtcta cgatctggcg gggttcatgg taggcggggt tgcggaggac
      181 gagatcgtcg atggttcggc gatccgccca ggcgacgtgc tggtcggcct gcccagctcc
      241 ggcttgcaca cgaacggttt ctcgctggcc cgcgccgcgc tcggcctcaa tggtccgccg
      301 gccgagctgc gcccgcgcct ggcgcagctc tcgctctggt ccgaccggtc gctcggtgag
      361 attcttttga cgccgcaccg ctcatacttg ccggtcatcg agccgctgct gggaaccggt
      421 ctcattcatg gcatggcaca catcaccggg ggcggcctgg ctggcaacat cggccgtgtc
      481 gttccgcaag acctccaggc ggtgatcgag ctcggctcgt ggcaggttcc gcgcctcttc
      541 ttggccattg aagacagcgg ccagatcgac gaggcggaga tgtaccgtgt cttcaatatg
      601 gggatcggct tcgtggtgat cctggccgcg aacgacatcg tgcggctgac cgagcaggaa
      661 gaagcggccg tcgtgatcgg tcacatcgtg cccagcgacg cccccgagcg cgtcacgctg
      721 gagagggccg gaggcgcgcc gtgagcgggt ttgaccgcag cctctcacgc ctggtcatca
      781 ccgacgaaga tcgaatgcag atcgtgacgt gcctccagcg cctgatgacc gactcgagcg
      841 ccagttactg catggtcatc gaccgcagcg ggcaggtgct ggcctgggag agcgaccagg
      901 agcgacccga gatgacctat ctcggtgcgc tgatcgccgc cacctacgcc tcgactcagg
      961 agatggctcg catcctcaag gaagatggct tcaagacgct gctgcaggag gggctgaagg
     1021 agcgtgtcta caccgagacc atcaaggatc gctggctgct ggtcgtgatt ttcgatcaac
     1081 agtctcaggt cggactggtc aaggtgctgg cgcggcgcga gagcgtcgtg ctggcggggg
     1141 tgctggacct gtcgcaggaa cgaggcgacc acccggacgc ccagcgtttc cggcgcatgg
     1201 gcaaggccac caccgacacg atcgatctca tcttcaaaga caacggctat tcggacgacg
     1261 agtaggatgt gactgcacca tggcgctgat caatgtggca gcccgtgaga tccacggcaa
     1321 gatcgtctac tacgggcctg gcctgtcggg caagacgacc aacctgcagt ttatccactc
     1381 gcgcatcccc aacaacacga agggggatct gttgtcgatc gagaccgaga ccgagcgcac
     1441 cctcttcttt gacttcctgc cgctcgatct cggcaaggtc cacggcttca gcattcgctt
     1501 ccatctctac accgtgccgg gtcagattct ctacgagcgc acgcgcgtgg cggtcctcaa
     1561 cggcgccgac ggcgtggtct tcgtggccga cgccggccgc gatcgcttgc cggacaactt
     1621 gcagagcctg cgcgagctgg cgcagaacct caccgcccag ggcaagcgct ttctcgatta
     1681 ccccttggtg atgcaataca ataagatgga cttgccggag gcgctgccga tcccggtcct
     1741 cgaccgctac ctcaacacca ttcgcgtgcc gcgcttcgaa gccgtcgcgc gcgacggcaa
     1801 gggtgtgttc gagacgctgc gcgcgatctc caagctcgtc gtcaacaagc tttagacgac
     1861 acgacggggc ccgacaaccg ctaccacgag gaaatcacct gatgccgaac ggcgccgctg
     1921 ccggcgagcg agatccgtcc ctagaacgcg cgcagagcgc cgcggcgatc cccacgcccg
     1981 agcccgagca tagtctccac gtggctgagc tgagcgtgca gatcttcgat cagctcgctg
     2041 acccgctggc actgccggcg ggtgagcgcg atctgctggc cgccgccgcg ctctggcacg
     2101 acgccgggca gcgcttcacc ctaccggagc atcatctgcg cagcttcgat atcatcgtca
     2161 accagcggct actgggcttc gacgacgagg atcgcctggc gatcgccaac atcgcacgct
     2221 accatcgcca atcgacaccc gagctggagc acaccggcta ccgcaatctc cggcgcgagc
     2281 gccggccgct ggtggatcgt atggcgtcga tcctccgcct ggccgaggcg ttggatgcca
     2341 gccacctgca attggtcgag cgcgtcgtcg ccaccgtctc gccgggtctg gtcgagttcg
     2401 acgtggtcgc agcgagctac cccactatcg aggtcgaacg cgcccaggcg cgcggcggcc
     2461 tcttccgtcg ggtcttccgc cgcgagacca gcttccgctg gcatcgccaa gccccaggga
     2521 gcgcgtgatg cccagggctc ttctcagcgt ctcggacaag tccggactcg ccgtttttgc
     2581 caggcggttg gcgcggctcg gcttcgagct catctcgacc ggcggcacgc tgcaggcgct
     2641 gactgacgct ggcgtgcccg ccatctccgt cgtcgacgtc accggcttcc ccgagatcat
     2701 ggcgggtaga gtcaagacgc tccacccggc gatccacgct ccgctgttgg cgcgtcgcga
     2761 cgtgccggaa gatctcgcca tcatggaaga gatgggcttc accggcatcg acctcctggc
     2821 cggcaacctc tacccgttcg cccagaccgt ggcgcggccc ggcgttacgc tggccgacgc
     2881 gctggagaat atcgacatcg gcggcccggc catgatccgc gccgccgcca agaactttcc
     2941 cgctgttctg gtcgtcgtcg accccgccga ctatgacccg ctcgcggacg cgatcgagcg
     3001 ggagtcgctc acgggcatcg acttggagcg tcgccaggca ctcgcggcca aagccttcgc
     3061 ccacgtctcg gcctacgacg ccgtcatcgc cgcgtacctg cggccggagg atcaattccc
     3121 ggagcgcatc accgtcgccg gcgagaagct ggccgacctg cgctacggcg agaacccgca
     3181 ccagccgggc gcgctctacc gccggctggc gcctggcccg gtcgccggcg tcggcgtctg
     3241 gcgcctgctg gctggccccg agatgtcgtt caacaactat ctcgacgcca atgccgcctt
     3301 gactgccgca ctcggcttcc ccgagcccac ctgcgccatc atcaaacacg ccaacccctg
     3361 cggcatcgcc agcgatcccg acctcggtgc cgcctacgaa gccgccctcg cctccgaccc
     3421 ggtctcagcc tacggcggca tcgtggcgct caaccggccg ctcgatatcg tcgccgccga
     3481 gcgcatcgcg gcaagccact tcgacatcgt catcgcgccc gatttcgttg aaggagcgct
     3541 cgcacgcctc cggcgaaaga agcggttgcg cctgctcgcc gcgccagcgc cgcaggtcgc
     3601 gagcgagccg gagattcgcc ccctcgaagg cggattcctg ctgcaagagc ccgaccggct
     3661 cccggtcgat cccagtgcct ggcagctcgt cagtcgccgg gcgccgaggg acgcggagat
     3721 ggatgcgctc gttttcgcct ggcgtgcggt tcgttacgtc aagtccaatg cgatcgtgct
     3781 ggcgcaaggc gaggccacgg tcggcatcgg cggcggacaa ccgaaccggg tcgatgccgt
     3841 gcgcatcgcg gtcgcacgtg ccggggagcg tgccgccggc agcgtcatgg ccagcgatgc
     3901 ctacttcccg ttccccgaca atatcgaggt ggccgccgcc gctggcgtcc gcgcggtcgt
     3961 ggcgcccggt gggtcgcgcc gcgatccgga ggttatcgcc gctgccgacg ccgccaacct
     4021 gacactcctc ttcacctcgg cgcggcactt ccggcactga ccgggaatgt ccgaccacga
     4081 agtgagcgcc ggtcacttca cgctcggcaa ctgggcaaca gcgaagagat ccttcggctg
     4141 cgacctcagg gtgaccgggt agcagtgagc ccctttctcc gtcatccctt cgacaggctc
     4201 agggcagact ctgagccgcc gcgagggatc tctccccggc ctctggtggc agtgaccgtc
     4261 agcctaacga cgtctcgccg tcccctacca gtacgcgacc ggccctctag gcgtaaccga
     4321 gcttgagcgc gaccgccagt gggcaacatg ggcgcctgcc cgtcaagcgc acagcatttt
     4381 cccttctggc aaacaccacg cacgcaactc ccaatcctgt gaccaagtct gtgggttctt
     4441 catggcccgc ggccgtcttg ccgcgcatgc tgccagcagg tcggttggcg accttagctg
     4501 gaaatcgaag ggagactcca atgcagacga cggttacatt acggcaattc aagcggcgcg
     4561 cccagacgac gattatcggg cttggcctgc tggcactggg gttcagcggc gcggtcgcac
     4621 tcggtcagac ggacctccgc caccagcacg cggcggcgcc ggtgctggcg gctcctgttt
     4681 cccaggggct ttcctacgat gacttccgct attacgacga aaacaccgaa ctgccgagca
     4741 gcgcgaacgc gcccgcgggg attggcgacg ccgcgcccgc tccgatcacg ccggatcgca
     4801 tgaagttctt ggagcagaac actcaactgc cggacacgca ggcgatcccg gtacagaggg
     4861 gtgggcctcg atagagcagg agacgatgtg gcgatacgct cgcgcaacgg cgagcgtatc
     4921 gc
//
LOCUS       DASUBI010000645         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1298902, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000645 DASUBI010000000
VERSION     DASUBI010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298902"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFU72_12430"
     CDS             <1..302
                     /locus_tag="VFU72_12430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5424343.1"
                     /translation="VDSSSIFPALRRAAIERVWVGIEAQAADEVPVLGKIPGVENLTV
                     ATGFSGHGFALSPIVGQLMSELIVDGDTSVPIEQLSIERFAGGSSPRAPRLRQVG"
     gene            367..519
                     /locus_tag="VFU72_12435"
     CDS             367..519
                     /locus_tag="VFU72_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424344.1"
                     /translation="MVLFIVLILVVFGVLSWLISRSRRKLLVVKEPPVTPRQAQQEEY
                     KDDTVG"
     gene            complement(602..790)
                     /locus_tag="VFU72_12440"
     CDS             complement(602..790)
                     /locus_tag="VFU72_12440"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEU5424345.1"
                     /translation="MLWLILLILLVILLVGSTPVWPYSRGWGYNPAGVLALLAVIILI
                     LWLLGVISFNAFIVAPTF"
     gene            1043..1312
                     /locus_tag="VFU72_12445"
     CDS             1043..1312
                     /locus_tag="VFU72_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424346.1"
                     /translation="MQPARRQLQQRGHERAETRDLLRQVFDRYWDDVAPLNNYTPETA
                     RLLLVREGGRDDRLVELGSDVNDPWLAVRLGLRERAAGRVLSRGH"
     gene            complement(1382..2620)
                     /locus_tag="VFU72_12450"
     CDS             complement(1382..2620)
                     /locus_tag="VFU72_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5424347.1"
                     /translation="MDALQFTDSIPRYALGKLAGRRRKGVYWSGLGSLRFLELPEPPL
                     PGPEWVRVATRYGGICGSDIGLIELHASTATAPFTSFPFTVGHENVGRIAEIGQAVKG
                     FQIGERVVVNPLLSCAARGFADDLCPACARGEPNLCECFRTGTLAPGMLTGFCRDTGG
                     SWSASFVAHQSQLLRVPDNLSDESALLAEPFAVALHAVLRNPPAEGQTVLILGAGVIG
                     LCTLAALRALALRARVIITARHPFQVAMAQRLGADEVVKPLRGAAFYRQIAGLTGAEV
                     LKPIIGKEVVRGGADVVYECVGSNTTVDDALRLTASGGTMVLVGLAGVPRGIDWTPIW
                     LNEVKVHGSYCYAIEDWCGERLPTMALALRLMAEGKADLAPLLTHTFRLADYRQALET
                     VTSKGTSGVIKAAFAFPDEP"
     gene            2947..>3490
                     /locus_tag="VFU72_12455"
     CDS             2947..>3490
                     /locus_tag="VFU72_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEU5424348.1"
                     /translation="MGEERGLATIRGRIERAQQVMTRQGVDYLFVTPSPDLVYLLDYP
                     AHASERMTLLAVPSSGQPLILVPELEAPRLEDKRPVLTVHPWGETEQPAALAANLLPH
                     LNGATIAISDVTWAVFLLRLMQAMPGARFVPGDTVLRELRMVKDAQELEYLRIAAQRT
                     DQVWQATLDRVPFAGQSETMV"
BASE COUNT          596 a         1144 c         1224 g          526 t
ORIGIN      
        1 tggtcgacag ctccagcatc ttcccggcgc tgcgacgggc ggcgatcgag cgcgtttggg
       61 tgggcatcga ggcgcaggcg gctgacgagg tgccggtgct gggtaagatc ccaggcgtcg
      121 agaacctgac ggtcgcgacc ggcttctctg ggcacggctt tgcgctgagt ccgatcgtcg
      181 gtcagctcat gagcgagctg atcgtcgacg gcgacacttc ggtcccgatc gagcagctct
      241 cgatcgagcg tttcgcgggc ggatcatcgc cgcgcgctcc gcgcttgcgc caggtagggt
      301 gaggcaaccg gcacggatat tgcttcggcg caccaaggat tgtggcagtc gtcgaaggag
      361 ccgctgatgg tgctgttcat cgtgctgatc ctggtggtgt tcggagtgct gagctggctg
      421 atcagccgta gccggcgcaa gctgctggtg gtgaaggagc cgccggtgac gccacggcag
      481 gcgcagcagg aagagtacaa agacgatacc gtcgggtagc gatcgacgcg gacgacggtc
      541 cggaccccag gcgagccgac gggccgcagc ccgccggctc gcttctccag cgctgtggcg
      601 cttagaacgt cggggcgacg atgaaggcgt tgaacgagat aacacccagc agccagagga
      661 tgaggatgat gaccgccagg agcgcgagta caccggccgg gttgtagccc cagccgcggc
      721 tgtaaggcca gaccggcgtc gagccgacca gcagaatgac gagtaagatc aagagaatca
      781 accagagcac gtatggtccc tttctctgca aacgagttgg ccgcgtggac gcgccggcac
      841 gcgggcggca accgccgtgg aagggcgcca acgcaccccg catgccatgc cgtgcctggc
      901 gccgcgaccg ttcgcggcga acccacccgt tcgtaccggg ggaatagccc ttcccggcta
      961 tgcctcggaa ggcgaccggt gcgctaagat cggtggtacg aggacgattg gcggtgagcc
     1021 gcaggcacgg gaggtcgcga acatgcagcc ggcacgacga cagctccagc agcgcggtca
     1081 cgagcgcgca gagacgcgcg acctgctgcg gcaggtgttc gaccgctact gggacgatgt
     1141 cgcaccgctg aacaattaca ctcccgagac ggcacgcctg ctgctggtgc gcgagggtgg
     1201 gcgcgacgac cggctggtcg agttgggcag cgacgtcaac gatccgtggc tggccgtgcg
     1261 gctcggcctg cgcgagcgcg ccgccgggcg cgtgctctcg cgcggccact gagccagcgc
     1321 ctatgcgtcg gagttcaatc cccgctccag ggagcggggt tttgttgtga tcctgaaccg
     1381 gtcatggttc gtcggggaag gcgaaggcgg ccttgattac tccgctggtg cccttggagg
     1441 tgacagtctc cagtgcctga cggtagtcgg ccaggcggaa ggtgtgcgtc aggagcggcg
     1501 ccaggtcggc cttgccctcg gccatcaggc gcagcgccag cgccatcgtc ggcaggcgct
     1561 cgccgcacca atcctcgatc gcgtagcagt agctaccatg aactttgacc tcattgagcc
     1621 agatgggcgt ccaatcgatg cccctgggca caccggccag ccccaccagt accatcgttc
     1681 cgccggaggc cgtcaggcgc agggcgtcgt ctaccgtggt gttcgagccc acgcattcgt
     1741 agacgacatc ggcgccgccg cgcacgacct ccttgccgat gatcggcttg agcacctccg
     1801 cgccggtcag cccggcgatc tgccggtaga aggccgcgcc acgcagcggt ttgaccacct
     1861 catcggcgcc caggcgttgt gccattgcga cctgaaaggg gtggcgcgcg gtgatgatga
     1921 cgcgcgcccg cagggcgagc gcgcgcaacg ctgctaacgt acacagcccg atgacgcccg
     1981 ccccgaggat caacaccgtt tgtccctctg ccggcgggtt gcgcagcacc gcgtgcagcg
     2041 ccaccgcgaa cggctcggcc agcagcgcgc tctcgtcgga gagattgtcc ggcacgcgca
     2101 gcagctgcga ctggtgcgcg acgaacgagg cgctccagct cccgccggtg tcgcggcaga
     2161 agccggtcag catgcccggc gccagcgtgc cggtacgaaa gcactcacag aggttcggct
     2221 cgccgcgcgc gcaggccggg cacaggtcgt cggcgaagcc gcgcgcggcg caggagagga
     2281 gcgggttgac cacgacgcgc tcaccgatct gaaacccctt gacggcctgc ccgatctcgg
     2341 cgatccgtcc gacgttttcg tggccgacgg tgaacgggaa cgaggtgaag ggcgcggtgg
     2401 cggtgctggc atgcagctcg atcaggccaa tgtcgctgcc gcagatgccg ccatagcgcg
     2461 tggcgacgcg cacccactcc gggccgggca acggcggttc gggcagctcg agaaaacgga
     2521 gagagcccag cccactccag tagacgccct tgcgacgccg cccggctagc ttgccgagcg
     2581 cgtaacgcgg gatgctatcg gtgaattgca gcgcgtccaa ggtcccccct cacgcgcgag
     2641 agtctacgca gccaggagac gctcgtccag cgggcggggc tgtcgacgac atttcggtgg
     2701 cggcctagga cgaatccttc gacagattca ggatgacatg gatggaggat ggaggcggac
     2761 gacgacgacg tgccgttctc gtacgccccg acccttaccc tgtcagcctg agcgcagtcg
     2821 atggatccgc cctctcccgg cgcccaacgt cgtcgaacgg cgcgtcacgg agccctacac
     2881 caaccgccgt tgagcgcgta gcatagccgt ggttgaagcc ggtgggactg aacgagggag
     2941 ggcgatatgg gcgaggagcg cgggctggcg acgattcgcg gccgcatcga gcgggcgcag
     3001 caggtgatga cacggcaggg agtcgactac ctcttcgtca ctccatcgcc cgacctcgtc
     3061 tacctgctcg actacccggc gcacgcctcg gagcggatga cgctgctggc ggtgcccagc
     3121 agcggtcaac cactgatcct tgtgccggag ctggaagcgc cccggttgga ggacaagcgc
     3181 cctgttctga cggtccatcc ctggggcgag accgagcagc ccgcagcact ggcggcgaac
     3241 ctgctgcccc atctgaacgg cgccaccatc gccatcagcg acgtgacctg ggccgtcttc
     3301 ctgctgcggc tcatgcaggc catgcccgga gcgcgcttcg tacccggcga tacggtgctg
     3361 cgcgagctgc gcatggtcaa ggatgcgcag gagctggagt acctgcgcat cgccgcgcag
     3421 cgcaccgacc aggtctggca ggccacgctc gaccgcgtgc cgttcgccgg ccagagcgag
     3481 acgatggtgg
//
LOCUS       DASUBI010000646         7281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_319507, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000646 DASUBI010000000
VERSION     DASUBI010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319507"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="trpS"
                     /locus_tag="VFU72_12460"
     CDS             complement(<1..513)
                     /gene="trpS"
                     /locus_tag="VFU72_12460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5424349.1"
                     /translation="MAGERPRVFSGIQPTGNIHLGNYLGAIRNWVRQQELYENIFCIV
                     DLHAMTLPYDAADLRARSLELGTVLLAAGIDPARSILFVQSDVREHSELAWVLGTLAG
                     FGELGRMIQFKEKSRGSEHVGAGLFYYPVLQAADILMYDAALVPVGEDQKQHIELTRD
                     LAIRFNNTFGE"
     gene            723..1310
                     /locus_tag="VFU72_12465"
     CDS             723..1310
                     /locus_tag="VFU72_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5424350.1"
                     /translation="MTFELQDLSDAELVQRMAEGDVVALEELYTRYARPVYSFAVRIV
                     GDNLVAEEILQEAFVRAWRQSDRFEFSRGSFPSWLLSITHNLAIDEVRRRQRRPQRAD
                     AVDISDVLRLEIDESANVEEAAEASELRERIQGAMAQLPQAQRRVIELAYFEGLTQRE
                     IAALLEEPLGTIKTRMRLAMQKLKEYLATEEGARS"
     gene            1310..2083
                     /locus_tag="VFU72_12470"
     CDS             1310..2083
                     /locus_tag="VFU72_12470"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5424351.1"
                     /translation="MQQEDGHRHVHDLIAPYVLGALEPDELEEVELHLLHCPACQALV
                     DEERQVALLLPYLAPPREVPRRARRQLLGRLHDDEPAPAGSLASRIPIGVLRISWFAA
                     TVAAVLALIFAVHSYQMQNQITSQNNQLLTLEGQQETVVSFVAEHGGFATRLQDTGMG
                     GSAQGAIIVDPNGNSAMMVVDGLTKPRPGHAYVVWVVRGQDHVKVGLLPVDNQGHAVL
                     RIVLPEAVVSFDSIIVTDESGPAVAFPSGTHLLSAHVNP"
     gene            complement(2256..4055)
                     /locus_tag="VFU72_12475"
     CDS             complement(2256..4055)
                     /locus_tag="VFU72_12475"
                     /inference="COORDINATES: protein motif:HMM:NF027401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4815 domain-containing protein"
                     /protein_id="HEU5424352.1"
                     /translation="MWRTRILSLAATLAMVATLFAGAVSLAAAAPITDASVMTPLYFP
                     WVPNGETLAGAGPWNGAVTIQNLEASSVVLGFNTTANTVVLNAHSSLQMSAAALHVAA
                     PGGLLVATASWNSADLPAGICTPGGTGTQQQSATVGTTRGVANGADVISLPAGQTLTS
                     VDAVTYGTTTYVVGTDYNVVQANDTTINIDWSLQGVGSKEPPTGATYSVKYTYTVQTT
                     TAADCSRAPHVAGAEKQALPTALTDLTTTANAKTVDGYTAVTTDGLGKTIYLPIVQRN
                     NNWESIIHVSNPNAAATTVTLDLTVAVNQSTGGNVTRSWTVGAGQTQTIDLASLVNSG
                     WIGSAKLSAPDSITALVDRVKPSTNMALINVAEPMGSQTVYAPLVFRGYQNWKTGINL
                     VDISGQSNKVTITYYRQDGTPLLSSNVTLPANGMNFVYVPEEAAAGENFLGSASLSGQ
                     GAFLVTLDEVKYTTTEAMSYLVTDTSQLPAAGDMLALPLIQKGDASGAGDTSGINLFN
                     PADASIQFTVTYFDTTGKQVATFNGALAPHQGQTLYTLDQGNLPAGFQGSAVIAVTGG
                     TGSLAAVSNNVNYAVAGDGSSVFSPLLITAPAP"
     gene            4482..5822
                     /locus_tag="VFU72_12480"
     CDS             4482..5822
                     /locus_tag="VFU72_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5424353.1"
                     /translation="MSVTDDATADAYGFHDPLTTLPNRALLMQRIKQALARSGRTERP
                     SAVLFIDLDNFKQINDTLGHQAGDHLLILIAGVLQASLRVSDTAARLAGDEFVVLLED
                     LNTPAEAALVAERILKQLASPFSFGEGGSGREAQVSASIGVAISSSPLDAPEELLHRA
                     DVAMYRAKQQGKNRFEVFDQRLSREIHERLRTEHDLRSALERGELRLLFQPEVRLEDD
                     AIVGVEALAHWERPGYGLLGPEEFLPLAEETGLSVSIGRWVLGEACRQAHGWRQSDPK
                     LVMSVNLSARAFYRAELVQEVAAALRESGLEPGGLALEIPEQVAMCDPERASAIFQRL
                     HGLGVKLALDRFGESCASLRHLRRWPLFFVKIDRPLVAELIDNRESAALVSTLIDVAH
                     ALDLRVLAEGVEIAAQAVWLRERRCDLAQGDYFARPSGSRMIGLLFGQDIGALA"
     gene            5994..6347
                     /locus_tag="VFU72_12485"
     CDS             5994..6347
                     /locus_tag="VFU72_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5424354.1"
                     /translation="MDREWLSVAETAAFLGISTRTIRRWIHQGKLDAELQPGRHGPQY
                     AIAREAAEALGRRDQPVPLDALTHQLAQLQHQFTLLQRQFEELRQLCLDLRHEHESLR
                     SRLADPARRAADENE"
     gene            complement(6375..>7281)
                     /locus_tag="VFU72_12490"
     CDS             complement(6375..>7281)
                     /locus_tag="VFU72_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5424355.1"
                     /translation="VPRRARPAGLLPRRSGEEEQPVPAETLRIGLASPRLAPAREAAV
                     ETVGRFLAEAAARDVAIVCFPETFLPGYRGDDFPAPPPDQPAQERALREVRALARAHR
                     VAVILPLEWSTAAGLLNLAVVIDADGALQGWQVKTQVAPEEEPYYVPGQGRRLFSVDG
                     VPFGIVICHEGFRYPETVRWAAQRGARVVFHPHLAGSDQQGVVPRHWGDPDAPYYEKA
                     MILRAVENEIFFASVNYAFHRQESATSLIAPDGRCLAHTPYGEEALLVSDLDLAAATG
                     QYARRFAPERFAGAPADDLRLAAPR"
BASE COUNT         1072 a         2443 c         2604 g         1162 t
ORIGIN      
        1 ctcgccgaag gtgttgttga agcggatggc caggtcgcgc gtcagctcga tgtgctgctt
       61 ctggtcctca ccgaccggca ccagggcggc gtcgtacatc aggatgtcgg ccgcctgcag
      121 caccggatag tagaagaggc cggcgccgac atgctcgctg ccgcgcgact tctccttgaa
      181 ctggatcatg cggcccagct cgccgaagcc ggccagcgtg ccgagaaccc aggccagctc
      241 gctgtgctcg cgcacatccg actgcacgaa gaggatcgag cgcgccgggt cgatcccggc
      301 cgccagcagc acggtgccca gctccagact gcgcgcgcgc aggtcggcgg cgtcgtaggg
      361 cagcgtcatg gcgtgcagat cgacgatgca gaagatattc tcgtagagct cctgctgccg
      421 gacccagttg cggatggcgc ccaggtagtt acccaggtgg atattgccgg tgggctggat
      481 gcccgagaag acgcgcggtc gctcgcctgc catccgtcgt tcgctctctc cctgcttcgt
      541 aacgccggca tgggccgccg tggttccggc cccaaacacc tattctatta taggcgtaag
      601 gatggtggga accgcacgag aggaggaggt tagaatgagg gcgacgaatc ctccgggtgc
      661 gggtggacgt agattgaagt gcaacaggcc ggccttgccg ggttggtgct gactagggcg
      721 gcatgacctt cgagctccaa gacctctccg atgccgagct ggttcagcgc atggccgagg
      781 gcgacgtcgt cgcgctggag gagctctaca ctcgctatgc gcgtccggtc tattcctttg
      841 ccgtgcgaat cgtgggggac aacctggtgg ccgaggagat cttacaggag gcgttcgtgc
      901 gggcctggcg gcagtccgat cgcttcgagt tctcccgcgg cagcttcccc agctggctct
      961 tgagcatcac ccataacctg gcgatcgacg aggtgcgccg gcgccagcgc cggccgcagc
     1021 gcgccgacgc ggtcgatatc agcgacgtgc tgcggctgga gatcgacgag tcggctaacg
     1081 tcgaggaggc cgccgaggcg tccgaattgc gcgagcgcat ccagggtgcg atggcccaac
     1141 tcccccaggc ccagcggcgg gtgatcgagc tggcctactt cgaggggctg acccagcgcg
     1201 agatcgccgc gctgctggag gagccgctgg gcaccatcaa gacgcgcatg cgcctggcga
     1261 tgcagaagct gaaggagtac ctggcgacgg aggagggcgc gcgcagctga tgcagcagga
     1321 ggacggccac cgccatgtcc acgatctgat cgcgccctac gtgctggggg cgctggagcc
     1381 ggacgagctc gaggaagtcg agctgcacct gctccattgc ccggcctgcc aggcgctggt
     1441 cgacgaggaa cggcaggtgg cgctcctgct cccctacctc gcaccgccgc gcgaggtgcc
     1501 gcgccgggca cggcgccaac tgctcgggcg cctgcacgac gatgagccgg cgccggcggg
     1561 ctcgctcgcc tcgcgtatcc cgatcggggt tctgcgcatc agttggttcg ccgccacggt
     1621 cgccgccgtg ctggccctga tcttcgccgt gcacagctac cagatgcaga accagatcac
     1681 cagccagaac aaccaactgc tgacgctgga gggccagcag gagaccgtcg tctccttcgt
     1741 agccgagcac ggcggctttg ctacgcgcct gcaagatacc ggcatgggcg gcagcgccca
     1801 gggcgccatc atcgtcgatc ccaacggcaa cagcgccatg atggtggtcg atgggctgac
     1861 caagccccgg cccggccacg cctacgtcgt ctgggtggtg cgggggcagg accacgtcaa
     1921 ggtgggcctg ctgccggtcg ataaccaggg ccacgccgtg ctgcgcatcg tgctccccga
     1981 ggcggtggtc agcttcgaca gcatcatcgt gaccgacgaa tccggccccg cggtagcctt
     2041 ccccagcggc acgcacctcc tctccgcgca cgtcaacccg taggccgggc cctcatcccc
     2101 agcccttctc ccaatgctgg gagaaaggag acgacgggct gtcggggcgg gtttgcatcc
     2161 cgccctcctc gtttgggtgc cgcgtggcgt acgcaacctc gtcggcatga gtagcccctc
     2221 tcccagtatt gggagagggg ttggggtgag ggccgctacg gcgcgggcgc ggtgatcagc
     2281 agcggcgaga agacgcttga gccgtcaccc gccaccgcgt agttgacgtt gttcgacacc
     2341 gccgccaggc tccctgtccc gccggtgacg gcgatcaccg ccgacccctg gaagccggcc
     2401 ggcaggttac cctgatcgag cgtgtagagc gtctgcccct ggtgcggcgc cagcgcaccg
     2461 ttgaaggtcg cgacctgctt gccggtggtg tcgaagtagg tcacggtgaa ctggatcgag
     2521 gcatccgccg ggttgaacag gttgatgccc gacgtgtcgc cggcgcccga ggcgtcaccc
     2581 ttctggatga gcggcagcgc cagcatgtcg cccgcggccg ggagctggct ggtgtcggtc
     2641 actagatagg acatcgcctc ggtggtggtg tatttgacct catccagcgt gaccaggaag
     2701 gccccctggc cggacagcga ggccgagccg aggaagttct cgcccgcggc cgcctcctcc
     2761 ggcacgtaga cgaagttcat gccgttggcc ggcagcgtca cattgctgct caggagcggc
     2821 gtgccgtcct gccggtagta ggtgatggtg accttgttgc tctggccgct gatgtcgacc
     2881 aggttgatgc cggtcttcca attctggtag ccacggaaga cgagcggcgc gtagacggtc
     2941 tggctcccca tcggctccgc cacgttgatc agcgccatgt tggtgctcgg cttgacccgg
     3001 tcgaccaggg ccgtgatcga gtccggcgcg ctcagcttgg ccgagccgat ccagccgctg
     3061 ttgaccaggc tggccaggtc gatcgtctgg gtctgcccgg cgccgaccgt ccagctccgg
     3121 gttacgttgc cgccggtcga ctggttcacc gccacggtca ggtcgagcgt gacggtggtc
     3181 gcggccgcgt tcgggttgct gacgtggatg atcgactccc agttgttgtt gcgctgcacg
     3241 atcggcaggt agatggtctt gccgagccca tcggtcgtga ccgcggtgta gccgtcgacc
     3301 gtcttggcgt tggcggtggt ggtcaggtcg gtcagtgcgg tcggcagcgc ctgcttctcg
     3361 gcgccggcga cgtgcggcgc gcgcgagcag tcggcggcgg tggtggtttg gaccgtgtag
     3421 gtgtatttga cgctgtaggt ggcgccggtc ggcggctcct tactgcccac cccctgcagg
     3481 ctccagtcga tgttgatggt ggtgtcgttg gcctggacga cgttgtagtc ggttcccacg
     3541 acgtaggtcg tcgtgccgta ggtcacggcg tccacgctcg tcaaggtctg tccggccggc
     3601 agggagatca cgtcggcgcc gttggccacg ccgcgcgtcg tccccacggt ggcggactgc
     3661 tgctgggttc cggtgccgcc cggcgtgcag atgcccgccg ggagatcggc gctgttccag
     3721 ctcgccgtgg ccaccagcag tccgcccggt gcggccacgt ggagcgccgc cgcgctcatc
     3781 tggagcgagc tatgggcgtt gagcaccacg gtattggcgg tcgtgttgaa gcccagcacg
     3841 accgagctgg cctccagatt ctggatcgtc accgcgccgt tccaggggcc ggcgcccgcc
     3901 agcgtctcgc cgttgggcac ccaggggaag tagagcggcg tcatcacgct cgcgtcggtg
     3961 attggggccg ccgcggccag gctgaccgcg ccggcgaaga gggtggcgac catcgccagg
     4021 gtcgccgcca ggctcagtat ccgggttctc cacatgaacg tgcctgctcc ttctcaatga
     4081 ctgctcaagg cgccccagcc ggcggagctt cgctcccagg ccgcgaggcg cacgaacaag
     4141 agccggtggg ctgtggctgc gcgctcctct ctgcgtccat cggactcggg gccggaatct
     4201 cgacagcgcg cgcccggcct cggcccggcg gcgcgcgacg atccgttgtg cggcggttcg
     4261 gcgcgcaaat tcgataccgg cgggtcggcg gccaccgggg cgggcgcggt cgggaaggaa
     4321 tctcggtcgg cctcgctcgc ggctaggccg gcggtacttc tggcctatga cctggcagac
     4381 gaacgtccgt tgtcggtccc aggggcgcct agtacatttg ggctagtgga tgtggacaac
     4441 tcggcacggc tgcttggtct gggtgggggt gggagcgaat gatgtcagtc acggacgacg
     4501 cgacggcgga cgcctacggc ttccacgacc cgctgacgac cctccccaac cgggcgctcc
     4561 tgatgcagcg catcaagcag gcgctggcgc gctcggggcg caccgagcga cccagcgcgg
     4621 tcctcttcat cgacctcgac aacttcaagc agatcaacga taccctgggc caccaggccg
     4681 gtgaccacct gctgatcctg atcgcgggcg tgctgcaggc ttctctgcgc gtcagcgata
     4741 ccgccgcacg cctggccggc gacgagttcg tggtgctgct ggaggatctc aacaccccgg
     4801 ccgaggcggc gctggtcgcc gagcgcatcc tcaagcagct cgcctcgccg ttctccttcg
     4861 gcgagggtgg gagtggacgc gaggcccagg tcagcgccag catcggggtt gccatcagca
     4921 gttcgccgct ggacgcgccg gaggagctgc tgcaccgcgc cgatgtcgcc atgtaccgcg
     4981 ccaagcagca gggcaagaat cgcttcgaag tcttcgacca gcgtctgagc cgcgagatcc
     5041 acgagcggct gcgcaccgag cacgacctgc gcagcgcgct ggagcgcggc gagctgcgcc
     5101 tcctcttcca gccggaggtg cggctggagg acgacgcgat cgtcggcgtc gaggcgctgg
     5161 cgcactggga acgccccggc tacgggctgc tcggcccgga ggaattcctg ccgctggccg
     5221 aggagaccgg gttgagcgtg tcgatcgggc gctgggtgct gggcgaggcc tgccgccaag
     5281 cgcacggctg gcgccagtcc gaccccaagc tggtgatgtc ggtcaatctc tcggcgcgcg
     5341 cgttctaccg cgcggagctg gtgcaagagg tggccgccgc gctgcgcgag agcggcctgg
     5401 agccaggcgg gctggcgctg gagatccccg agcaggtggc gatgtgcgac ccggaacgtg
     5461 ccagcgcgat cttccagcgg ctgcacgggc tgggtgtcaa gctggcgctc gaccgcttcg
     5521 gcgagtcgtg cgcctcactg cggcacctgc gacgctggcc gctcttcttc gtcaagatcg
     5581 accgtccgct ggtggccgag ctgatcgaca atcgcgagag cgcggcgctg gtgagcactc
     5641 tgatcgacgt ggcccacgcg ctcgatctgc gcgtgctggc cgagggggtc gagatcgcgg
     5701 cccaggcggt ctggctgcgc gagcgccgct gcgatctggc ccagggcgac tacttcgcgc
     5761 gcccctcggg cagccgcatg attggtctcc tcttcgggca ggacatcggc gcgctcgcct
     5821 gagacgcgcc gtctggcaca tcatctgcag gcgaacgggg ccgaaccggc ccaagccggc
     5881 gtggcagagc gcgacggctt ggttccccgg gcggtgcgca gcatgacggg ccggtcgggc
     5941 gcgaccccgc atgacgctgc cgggtatcgc tcgcggtaga gcgaggaaag ccggtggatc
     6001 gcgagtggct tagcgtggcc gagacggccg ctttcctggg aatctcgacg cgaaccattc
     6061 ggcgctggat tcaccagggc aaactggacg cggagctaca gccgggacgt cacggcccgc
     6121 agtacgcgat cgcgcgcgag gcggccgagg cgctgggccg gcgggaccaa cccgtgccgc
     6181 tcgacgccct cacgcaccag cttgcccagc tccagcacca gttcacgctc ctgcagcggc
     6241 agttcgagga gctgcggcag ctctgcctcg acctgcgcca cgagcacgag tcgctgcgca
     6301 gccggctggc ggatccggcc cggcgagcag ccgacgagaa cgagtagggc gccgccgccc
     6361 gtctgccggc tcgctcaacg cggcgcggcg agcctgaggt cgtcggccgg cgcgcccgcg
     6421 aagcgctccg gggcgaagcg ccgggcgtat tggcccgtgg cggccgccag atcgagatcg
     6481 gacaccagca gcgcttcctc gccgtagggc gtgtgtgcca agcagcggcc atccggcgcg
     6541 atcaggctgg tggccgactc ttgacggtgg aacgcgtagt tgacgctggc gaaaaagatc
     6601 tcgttctcga ccgcccgcag gatcatcgcc ttctcatagt agggcgcgtc cgggtcgccc
     6661 cagtggcgcg gcacgacgcc ctgctggtcg ctgccggcca ggtgcgggtg aaacacgacc
     6721 cgcgcgccgc gctgcgcggc ccagcgcacg gtctcggggt agcgaaaccc ctcgtggcag
     6781 atcacgatgc cgaagggcac accgtcgacg gagaagaggc gccggccctg gccggggacg
     6841 tagtagggct cctcctcggg agcgacctgc gtcttgacct gccagccctg gagcgcgccg
     6901 tcggcgtcga tgacgacggc caggttgagc agtccggcgg cggtcgacca ctccagcggc
     6961 aagatcaccg cgacccgatg ggcgcgggcc agcgcgcgga cctcgcgcag cgcacgctcc
     7021 tgcgccggct gatcgggcgg tggcgccggg aagtcgtcgc cgcggtagcc gggcaaaaac
     7081 gtctcgggaa agcagacgat ggccacgtcc cgcgccgccg cctcggccag gaaacggccg
     7141 accgtctcga ccgcggcctc gcgcgccggc gccaggcgcg gcgacgccaa tccgatccgc
     7201 agtgtctcag ccggcaccgg ctgctcctct tctccagatc gtcgcggcag cagtccggcg
     7261 ggtcgagcgc ggcgggggac c
//
LOCUS       DASUBI010000647         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1055696, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000647 DASUBI010000000
VERSION     DASUBI010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055696"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFU72_12495"
     CDS             <1..320
                     /locus_tag="VFU72_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5424356.1"
                     /translation="LLKQGYTILERKWRVRTGEIDLIALDGDVLVFCEVKTRRGAARG
                     AAEEAVDAAKAARLLALGEEYVETHPEHGERFWRVDLLALTLAPDGRVARVSHIVDAI
                     QSE"
     gene            347..856
                     /locus_tag="VFU72_12500"
     CDS             347..856
                     /locus_tag="VFU72_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEU5424357.1"
                     /translation="MSESVEQHRAAAPEQVRCAVVTVSDTRTVETDRSGQLIQEKLRA
                     AGHEIMDYAIVKDEPDQIIDALSRLVAHPDCDAIIFNGGTGIARRDTTFDAVDARLQK
                     RLDGFGELFRSLSYAEIGAAAMLTRATAGVINGTVIFLTPGSSNAVALAMDKLIGPEL
                     AHVVFEMRK"
     gene            913..2247
                     /gene="hisS"
                     /locus_tag="VFU72_12505"
     CDS             913..2247
                     /gene="hisS"
                     /locus_tag="VFU72_12505"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5424358.1"
                     /translation="MSGGTGSVRVKPQVLKGMRDFLPGQMILRQRVMDTWRGVFERYG
                     FAPIETPTIEYLEILTGKIGEDEKLIYHFEDHGGRPVGLRYDLTVPLARFIASHRHEL
                     TFPFKRYHIAPVFRADRPQKGRYRQFWQCDVDIVGTTSPVADAEIIMIWITALAAIHM
                     PNVVVQINHRKLLQSLSTYAGAQPGQAQAIIRAIDKLAKIGPSGVEEEMLRHGVPAEA
                     ARRVLELVALRGEPAEVLAELRQRLAGLDEAQAGVSDLDLIFGYLRDMGANPSQYTLD
                     LSLARGLDYYTGPVFEAVVEEPKIGSLGGGGRYDGLIGTFLGEEIPATGAALGLERIV
                     DVIEELALLPQPEAVSKVLVTIFGDELIGASLALVEELRRAGVPAEIYLGESRQLRRQ
                     LEYANKRGIPVVAILGPDEVANGTVMLRRMATGEQDATPRGEAAAAVLRLAA"
     gene            2254..>2506
                     /locus_tag="VFU72_12510"
     CDS             2254..>2506
                     /locus_tag="VFU72_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424359.1"
                     /translation="MPLLGNSGSPRWHDGRFADYLALLEASGATLVELPLHHGPHDER
                     TARVHVLEADWDAVVEAYRARDIAVQLHVSLDPRFATERW"
BASE COUNT          402 a          817 c          912 g          375 t
ORIGIN      
        1 tgctgctgaa gcagggctat acgattctgg agcgcaagtg gcgcgtgaga accggcgaga
       61 tcgacctgat cgcgctggat ggcgacgtgc tcgtgttctg tgaggtcaag acgcggcgag
      121 gcgccgcgcg gggtgcggcc gaggaagcgg tggatgcggc caaagcggcg cgcttgctgg
      181 cgctggggga ggagtacgtc gagacgcacc ccgagcacgg cgagcgcttc tggcgggttg
      241 acctgctggc gcttacgctg gcgccggacg ggcgcgtggc gcgtgtcagc cacatcgtag
      301 atgcgatcca gagcgagtga gccgggcaaa cgaagggggc ggccaggtga gtgagtcggt
      361 cgagcagcat cgggcggcgg cgccggagca ggtgcgttgt gcggtggtca cggtcagcga
      421 cacgcgcacg gtcgagacgg accgcagcgg acagttgatc caggagaagc tgcgcgccgc
      481 cggacacgag atcatggact acgccatcgt caaggacgag ccggaccaga tcatcgacgc
      541 gctgagccgg ctggtggcgc accctgactg cgacgcgatc attttcaacg gcggcaccgg
      601 catcgcgcgg cgcgatacga ccttcgacgc ggtcgacgcg cgtctgcaaa agcgcctcga
      661 cggcttcggc gagctctttc gctcgctgag ctacgccgag atcggcgcgg ccgccatgct
      721 gacccgtgcc accgccggcg tgatcaacgg cacggtgatc ttcctcacgc ccggctcttc
      781 caacgccgtc gcgctggcga tggacaagct gatcggcccg gagctggcgc acgtcgtctt
      841 cgagatgcgc aagtagaccg gctggtatca tcgaacgatt acgaggcggg ccatgctgga
      901 aggagtgcct ggatgagcgg aggtaccggc tccgtgcggg tcaagccgca ggtcctcaag
      961 gggatgcgcg actttctgcc ggggcagatg atcctgcgcc agcgggtaat ggacacctgg
     1021 cgcggcgtct tcgagcgcta cggcttcgcg ccgatcgaga cgccgaccat cgagtacctg
     1081 gagattctca ccggcaagat cggcgaggac gagaagctca tctaccattt cgaggatcat
     1141 ggcgggcgcc cggtcgggct acgctacgac ctgacggtgc cgctggcgcg cttcatcgcc
     1201 tcgcaccggc acgagctgac cttccccttc aagcgctacc acatcgcgcc ggtctttcgc
     1261 gccgaccgcc cccagaaggg gcgctaccgc cagttctggc agtgcgacgt cgatatcgtg
     1321 ggcacgacct cgccggtcgc cgacgccgag atcatcatga tttggatcac cgcgctggcg
     1381 gcgatccaca tgccgaacgt cgtcgtgcag atcaaccacc gcaagctgct gcagagcctc
     1441 tctacgtatg cgggggcgca gcccggccag gcgcaggcca tcatccgggc catcgacaag
     1501 ctggcgaaga tcggtccgag cggcgtcgag gaggagatgc tgcgccacgg cgtgccggcc
     1561 gaggcggccc ggcgcgtgct ggagctggtc gcgctgcgcg gagagccagc ggaggtgctg
     1621 gccgagctgc gccaacggct ggcagggctt gacgaggcgc aggcgggcgt cagcgacctg
     1681 gatctgatct tcggctatct ccgcgacatg ggcgccaatc cgagccagta cacgcttgac
     1741 ctgtctctgg cgcgcgggct cgactactac accggcccgg tcttcgaggc cgtggtcgag
     1801 gagccgaaga tcggctcgct cggcggcggc gggcgctatg acggcctgat cgggacattc
     1861 ctcggcgagg agattccggc aaccggggcc gcgctggggc tggagcgcat cgtcgacgtc
     1921 atcgaagagc tcgccctgct gccccagcct gaggcggtct cgaaggtgct ggtcaccatc
     1981 ttcggcgacg agctgatcgg cgcctcactc gccctggtcg aggagctgcg gcgcgcgggc
     2041 gtgccggccg agatctacct gggcgagagc cgccagctgc ggcgccagct ggagtacgcc
     2101 aacaagcggg gcatcccggt ggtggcgatc ctcgggccgg acgaagtagc gaatggcacg
     2161 gtcatgctgc gccggatggc gaccggcgag caggacgcga cgccgcgtgg cgaggcggca
     2221 gccgctgtgc tgcgcctggc cgcctgagcc gacgtgccgc tcctgggcaa cagcggctcg
     2281 ccccgctggc acgacggccg cttcgcagac tatctggcgc tgctggaggc gtccggcgcg
     2341 accctggtcg aactgccgct gcaccacggc ccgcatgacg agcgcaccgc ccgcgtgcac
     2401 gtgctcgaag ccgactggga cgcggtcgtc gaggcctacc gcgcgcgcga catcgcggtg
     2461 cagttgcacg tctcgctgga tccgcgcttc gccaccgagc gctggc
//
LOCUS       DASUBI010000648         8789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1077388, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000648 DASUBI010000000
VERSION     DASUBI010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077388"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU72_12515"
     CDS             complement(<1..234)
                     /locus_tag="VFU72_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424360.1"
                     /translation="MIDACLLGIGGFMPLPGRWLSALLLRSGRDVVLCDCGEGTQISW
                     KETNWGFRDVATIVLSHLHADHVAGLPGILFMLS"
     gene            complement(250..930)
                     /locus_tag="VFU72_12520"
     CDS             complement(250..930)
                     /locus_tag="VFU72_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5424361.1"
                     /translation="MATTSTAQEVDAVKQSIINAATQLVQSGVLSRSQHGNISARLPD
                     GDHFVLTGGGTLESLSAGDLAVLDLQGNVVEGYMSPMSAEIIQMHAAVYRKRDDVGSV
                     IHTHSPYVTAFAIAGKEIPTAYEAMVRFDFTEPVPVARYGPRGSEKSINNIVDVVGPN
                     TKAVLLENHGLLAFDSDIEKTVRGIMVLEESAEIILLADELGGAKPIPPEMVHATNER
                     RDQFGGRR"
     gene            1110..2705
                     /gene="ggt"
                     /locus_tag="VFU72_12525"
     CDS             1110..2705
                     /gene="ggt"
                     /locus_tag="VFU72_12525"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5424362.1"
                     /translation="MPGRPTTLATEGMIATPHYLASIAGLDVLRGGGNAVDAAIAANA
                     VLTVVYPHMCALGGDLFALIWDPAERQLAALNASGRAPAGQSIAALRDLGYAEMPDRG
                     PQAVTVPGAVDGWATLLDRFGSWPLGTLLQAAIGYAERGFPVSPVLSAGMTLGAELLA
                     RQPATAAQFLPDGQPLAPGDVLRQPDLGRSLRLLAETGPDALYRGPLGAAMARTVQAA
                     GGFLSEADLAVQRAEWVSPLATTYRGVELIELPPNTQGITALTLANIVEGWDVAALGH
                     NSPATIHRFVEAKKLAFADRDQFITDPEFAAIPVEQLISKAHAADLRAQIDLGRASQP
                     SDRPGGGDTIYLCVVDGNGLCVSLIQSIFSSWGSGMIAERTGILMHNRGFSFTLDPRA
                     ANRLEPRKRPMHTLIPALLMRDGLPWIVFGTMGAHGQAQTHLQLLTNLIDFGLEPQAA
                     IEAPRWVSGRGLAGDPANVLAIEDRVAPEVIERLRVLGHEIRILPAFSSLLGHANMIQ
                     IDRQRGVLMGASDPRSDGAALGW"
     gene            complement(2922..4277)
                     /locus_tag="VFU72_12530"
     CDS             complement(2922..4277)
                     /locus_tag="VFU72_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5424363.1"
                     /translation="MDRLLDSTLRRAQRYLDGLAERPVAPSPAALDRLPGLGGSLPEE
                     PVDPADVLALLDELGSPATVASAGPRYFGFVTGGSLPAALAANWLAGAWDQNAGLPVT
                     SPAGTALEEIALDWLLDVLNLPPGTAGAFVTGATMANFTGLAAARHAVLAQAGWDVEA
                     HGLFGAPPITVVVGAEVHVSLLKALALLGLGRERVLAVPTDGQGRMRADELPEISGPA
                     IVCLQAGNVNTGAFDPAAEVCAWARGTGAWVHVDGAFGIWAAAAPSLAGLLAGVNQAD
                     SWATDAHKWLNVPYDSGLAFVRDRAALLPAMAASAAYLPEGESREPMRYTPELSRRAR
                     GAEVWAALRSLGRAGLADLIERDCRYARRFAEGLSAAGYEVLNEVVLNQVLVAFGDDD
                     ATNRVMAGVQAEGTCWCGGTVWHGRAAMRISVSSWATTEDDVEQSLAAIIRVARAETK
                     A"
     gene            complement(4529..5941)
                     /locus_tag="VFU72_12535"
     CDS             complement(4529..5941)
                     /locus_tag="VFU72_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5424364.1"
                     /translation="MRTLSIRRWLLLSLLALFVVPILLFRLSVPLLGAAPQWFGSARQ
                     SRDLDQAAALLSAPARWSDREWQRSTRATLADLHVAAEIHDPQGGLIFSTSDQSTGGA
                     PWSHGWGSAAGLSREIQVVADGAPAGTIDLASTSSGWPASFGGAALAALLGLALTLVV
                     AGWLIGRYVVRPLEATSQAARQIASGDLDFDLPDSRVREVAEMRAAFQAMGAGLRDSL
                     RRQAELEEERRFFISAIAHDLRTPLFSLRGYLLGLERGIASTPEKVAHYVEVCRQKAD
                     QLDRLVADLFAYTKVEYLEETLRVGEEEQELGELLRGVAEGLQPRARAREVTLRLEGS
                     DQGCPLAVDRHLLERAVENLLDNALRHTPNGGAIEVAWHAEPGRAVFTVADSGPGIAE
                     QDLQHLFEPLFRGEVSRNRATGGVGLGLTIARRILRAHGGDLSAANRPAGGALFTAWL
                     PCRLERAAPAAPTAGEVPVS"
     gene            complement(5938..6630)
                     /locus_tag="VFU72_12540"
     CDS             complement(5938..6630)
                     /locus_tag="VFU72_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424365.1"
                     /translation="MNEERTVLVVDDDPAIVELMRDFLEAEGYRVRAAGDAAAAASLL
                     EQEPVDCVLLDVMMPGESGFELCRRLRRSSDVPILFLSARGEDVDKIRGLGLGGDDYI
                     VKSATPAEVVARVKAVLRRSGGATKARPALLDFGRLALDPAAREVWVESRSVSLTPKE
                     FELLSLLAAHPRQVFTYDQLLERCWEGIGDRHTVTVHVGRIREKIEAAPERPRLIVNV
                     WGVGYRFEGQPR"
     gene            6869..7288
                     /locus_tag="VFU72_12545"
     CDS             6869..7288
                     /locus_tag="VFU72_12545"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEU5424366.1"
                     /translation="MRRGLAVIFAALAGLVLVAVVAQFFLAGAGLFGAVSGYQSHEIV
                     GMLIATASIVLLLLAIFGRFGRAFIIHAAALLVLLLVQIALIESDQPWLEALHPVNAL
                     LILGASAQLAQRAWAWVRRPGPVAASVAERVLRERGF"
     gene            7292..7567
                     /locus_tag="VFU72_12550"
     CDS             7292..7567
                     /locus_tag="VFU72_12550"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5424367.1"
                     /translation="MGLSLGLLAVAGDPAVGWAHAGHGFVWGPIVLFFWIGALIMFAW
                     FVARGPWMRGGQRGPHGVERAREILAERYARGEISTEEYWERREQLL"
     gene            7676..>8789
                     /locus_tag="VFU72_12555"
     CDS             7676..>8789
                     /locus_tag="VFU72_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEU5424368.1"
                     /translation="MRVKLNQPARSYLASLVAVAGTVALLLPFRAALATLSVALIFLI
                     VVTAVALYGNTWSGVLASLTAFLCFDFVFIPPYYRLNVAHLDDVLVLFVFLGIAVLTS
                     QLVVRVRQQTDEALRRGHETTSLYDLSIALIGESSLPEMLTTIARHMERLFALDSCSV
                     LLDERVSPSGSSASAAQLAEQSTPRTEHVPGTLAIPVGTARRALGVLVVSRRPPRAVF
                     DLGERRLLETFANQAAIAIERIILTEEETRAEVLAESDALKSTLLSAVSHELRTPLAS
                     IKASATSLLQPGIEWAEVDRRELLEAIDEESDRLNRLVANLLDLSRIEAGVLRPAFDW
                     CEPGEVIYEAVNRCETSLRGHRLALAIPDDLPPLRID"
BASE COUNT         1240 a         3060 c         3171 g         1318 t
ORIGIN      
        1 cgagagcatg aagaggatac cggggagccc ggcgacgtgg tcggcgtgca gatggctcag
       61 cacgatggtg gccacgtcgc ggaagcccca gttggtctct ttccaggaga tctgcgtccc
      121 ctcgccgcag tcgcacagca cgacgtcgcg gccggagcgc aggagcagcg cggagagcca
      181 gcggccgggg agcggcatga agccgccgat cccgaggaga caggcgtcga tcacccggcc
      241 gcggcccgcc tagcgccggc cgccgaactg gtcgcggcgc tcgttggtgg cgtggaccat
      301 ctccggcggg atcggcttgg cgccgcccag ctcgtcggcc agcaggatga tctcggccga
      361 ctcctccagc accatgatgc cgcgcaccgt cttctcgatg tcgctgtcga aggccagcag
      421 gccgtggttc tccagcagga cggccttggt gttcggcccg accacgtcga cgatgttgtt
      481 gatcgatttc tcggagccgc gcgggccgta tctggcgacc ggcaccggct cggtgaagtc
      541 gaagcgcacc atcgcctcgt aggcggtggg gatctccttg ccggcgatgg cgaaggcggt
      601 gacgtagggc gagtgggtgt ggatgaccga gccgacgtcg tcgcgcttgc ggtagacggc
      661 ggcgtgcatc tggatgatct cggccgacat cgggctcatg tagccctcga ccacgttccc
      721 ttgcaggtcg agcaccgcca aatcaccggc gctgagcgac tccagcgtgc cgccgccggt
      781 cagcacgaag tggtcgccgt ccggcagccg ggcgctgatg ttgccgtgct gcgagcggga
      841 gagcacgccg ctctggacaa gctgcgtggc cgcgttgatg atcgactgct tgacggcatc
      901 aacttcctgc gccgtcgagg tggttgccat cacgtacctc ccttgcgtgc tacgaggcag
      961 ccaatggagc cattgtcgtc gtcgccccgc gttcccgcaa ggcccgcgcc agccagcggg
     1021 cttgtgccga gcggcaggac cggcgaagat cgcgccgacg gccattgacg cgaatggtga
     1081 cgaggggcag gaggaggagc accgtgcaga tgccgggccg cccgaccacg ctggcaaccg
     1141 agggcatgat cgccacgccg cactacctgg cgagcatcgc cgggcttgac gtgctgcgcg
     1201 gcggcggcaa cgcggtcgac gccgccatcg cggccaacgc ggtgctgacg gtcgtctacc
     1261 cgcacatgtg cgccctcggc ggcgacctct tcgcgctgat ctgggatccg gcggaacggc
     1321 agctggccgc gctcaacgcc agcgggcgtg cgccggccgg gcagtcgatc gccgcgctgc
     1381 gcgatctcgg ctacgcggag atgcccgacc gcgggccgca agcggtgacg gtgcccggcg
     1441 cggtcgatgg ctgggccacg ctgctcgatc gcttcggctc ctggccgctg ggcacgctgt
     1501 tgcaagccgc catcggctac gccgagcgcg gtttcccggt ctcgccggtg ctcagcgccg
     1561 gcatgactct cggcgccgag ctgctcgcgc gccagcccgc cacggccgcg cagttcctgc
     1621 ctgacgggca gccgctggcg ccgggcgacg tcctgcgcca gccggacctg ggacgctcgc
     1681 tgcggctgct ggcggagacg gggccggacg cgctctaccg cgggccgctg ggcgcggcga
     1741 tggcgcgtac ggtgcaggcg gcgggcggct tcctgagcga ggctgatctg gcggtgcagc
     1801 gggccgagtg ggtcagcccg ctggccacga cctaccgcgg cgtggagctg atcgagctgc
     1861 cgcccaacac gcaggggatc accgcgctga cgctggccaa catcgtcgag ggctgggacg
     1921 tggccgcgct gggccacaac tcgcccgcga cgatccaccg cttcgtcgag gccaagaagc
     1981 tggcgttcgc cgaccgcgac cagttcatca ccgaccccga gttcgccgcc atcccggtcg
     2041 agcaactgat ctcgaaggcg cacgccgccg acctgcgcgc ccagatcgac ctcgggcgcg
     2101 cgtcccagcc gtccgaccgg cccggcggcg gcgacacgat ctacctctgc gtggtcgacg
     2161 gcaatgggct gtgcgtctcg ctgatccaga gcatcttctc gagctggggc agcggcatga
     2221 tcgccgagag gaccgggatt ctgatgcaca accggggctt ttcgttcacg ctcgatcccc
     2281 gggccgccaa ccggctggag ccgcgcaagc gcccgatgca cacgttgatc ccggcgctgc
     2341 tgatgcgtga cggcctcccc tggatcgtct tcggcaccat gggcgcgcac ggccaggcgc
     2401 agacgcacct gcaattgctg acgaatctga tcgacttcgg cctggagccg caggcggcga
     2461 tcgaggcgcc gcgctgggtc agtgggcgcg ggctggcggg cgacccggcc aacgtgctgg
     2521 cgatcgagga ccgcgtcgcg ccggaggtga tcgagcggct gcgcgtcctg ggccacgaga
     2581 tccggatcct gccggccttc tcgtcgctgc tgggccacgc caacatgatc cagatcgatc
     2641 gccagcgcgg cgtgctgatg ggcgcgtccg atccccggtc ggatggtgcg gcgctgggct
     2701 ggtgatgggc acggtggcct tcgtgcggag tgagcgccgc gtacggcgcg gatccttcca
     2761 cttcggtcgg gatgacagag ttagtaagga actgacgagg aggatcggcc tagaatgacg
     2821 atgcgcctgg tccggtaggc catccccttt tccctgtcat cctgagcttg tcgaaggatc
     2881 cgtccgcgcg cacgcctcaa cgaattctcc gggcacatgc cctacgcctt cgtttctgcc
     2941 ctggccacgc ggatgatcgc cgctaggctc tgctcgacat cgtcttccgt cgtggcccag
     3001 gaggagacgc tgatgcgcat ggcggcgcgg ccatgccaga cggtgccgcc gcaccagcag
     3061 gtgccctcgg cttgcacgcc ggccatcacc cggttggtgg catcgtcgtc gccgaaggcg
     3121 accagcacct ggttgagcac gacctcgttg agtacctcgt agccggcggc ggataaaccc
     3181 tcggcgaagc ggcgggcgta gcggcagtcg cgctcgatca ggtcggccag cccggcgcga
     3241 ccgagcgagc gcagcgcggc ccagacctca gcgccgcgcg cccggcgcga tagctcgggc
     3301 gtataccgca tcggctcgcg ggactcgcct tcaggaaggt aggccgcgga cgctgccatg
     3361 gccgggagca gggcggcgcg gtcgcgcacg aaggcgagcc cactgtcgta gggcacgttc
     3421 agccacttgt gggcgtcagt ggcccaggag tcggcctggt tcactcccgc cagcagcccc
     3481 gccaacgacg gcgcggcggc ggcccagatg ccgaaggcgc cgtcgacgtg cacccaggcg
     3541 cccgtcccac gcgcccaggc gcagacctcg gcggcggggt cgaaagcgcc ggtgttgacg
     3601 ttgccggcct gcaggcagac gatcgcgggc ccgctgatct ccggcagttc gtccgcgcgc
     3661 atgcgtccct ggccgtcggt tggcacggcg agcacgcgct cgcggcccag ccccagcagc
     3721 gccagcgcct tgagcagcga gacgtgcacc tcggcgccca cgaccacggt gatcggcggg
     3781 gcgccgaaga gcccgtgcgc ctctacgtcc cagccggcct gggccaggac ggcgtgccgg
     3841 gcggcggcca ggccggtgaa gttggccatg gtcgcgccgg tgacgaaggc gccggccgtg
     3901 ccgggcggca ggtttagcac gtcgaggagc cagtcgaggg cgatctcctc cagcgcggtg
     3961 ccagccgggc tggtcaccgg aagcccggcg ttctgatccc aggcgccggc cagccagttg
     4021 gccgccagcg cggcggggag gctgccgccg gtgacgaagc cgaagtagcg cggtccggcc
     4081 gaggcgacgg tggccggcga gcccagctcg tcgagcaggg ccagcacgtc ggcgggatcg
     4141 accggctcct cggggagcga gccacccagg ccgggcagac ggtcgagcgc ggcaggggag
     4201 ggcgccaccg gacgctcggc gagcccgtcg aggtagcgct gtgcacggcg cagggtagag
     4261 tcgagtagcc ggtccatgag atcgcccctc ctgtcgctgg ttccttaccg tgtcggtcgt
     4321 ctcgccgatt atcgcatgaa ggctggatca cgagcttccg acgggcgagg ggctcgtgag
     4381 gacgaatcct tcgactgcgc tcagagtctg cccccagctt gtcgaaggga cgacaggggg
     4441 agaggatggg gcgcacgacg acgcgtcgta ttcgtccgcc cctaactctc cctgtcatcc
     4501 cgaccgaagt ggagggatcc gtgttgccct acgaaaccgg cacctcgccg gccgtcgggg
     4561 cggccggcgc cgctcgctcc agccggcagg gcagccaggc ggtgaagagg gcgccgccgg
     4621 cggggcggtt ggcggcgctg aggtcgccgc cgtgggcgcg caggatgcgc cgggcgatgg
     4681 tcagacccag gcccacgccg ccggtggcgc ggttgcgcga gacctcgccg cggaagagcg
     4741 gctcgaagag gtgctgcagg tcttgctcgg cgatccccgg gccactatcg gcgaccgtga
     4801 agaccgcccg tcccggctcc gcgtgccagg cgacctcgat ggcgccgccg ttcggcgtat
     4861 ggcgcagcgc gttgtcgagc aggttctcga cggcgcgctc cagcaggtga cggtcgacgg
     4921 ccagcgggca gccctggtcg gagccctcaa gccgcagcgt cacctcccgc gcccgtgccc
     4981 gcggctggag accctcggcc acgccgcgca gcagttcgcc caactcctgc tcctcctcgc
     5041 cgacgcgcag cgtctcctcc aggtattcga ccttggtgta ggcaaagagg tcggccacca
     5101 gccggtcgag ctggtccgcc ttctggcggc agacctcgac gtagtgcgcc accttctccg
     5161 gcgtggacgc gatgccgcgc tccagcccca gcagatagcc acgcagggaa aacaggggcg
     5221 tgcgcagatc gtgggcgatg gcgctgatga agaagcggcg ctcctcctcc agctcggcct
     5281 gccgccgcag cgagtcgcgc aacccggcgc ccatcgcctg gaaggcggcg cgcatctcgg
     5341 ccacctcgcg cacgcgcgag tcgggcaggt cgaagtcgag atcgccggac gcgatctgcc
     5401 gcgccgcctg gctggtcgcc tccagcggcc gcaccacgta gcgcccgatc agccagccgg
     5461 ccacgaccag cgtcagcgcc agcccgagga gcgccgccag cgccgcgccg ccgaacgagg
     5521 cgggccagcc gctgctggta ctggcgaggt cgatcgtgcc cgccggcgcg ccatcggcga
     5581 cgacctggat ctcgcggctg aggcccgccg ccgagcccca gccatgggac cacggcgcgc
     5641 cacccgtcga ctggtcgctc gtgctgaaga tcaggccgcc ctgcggatcg tggatctcgg
     5701 cggcgacgtg caggtccgcc agcgtggcgc gcgtggatcg ctgccactca cggtcgctcc
     5761 agcgcgccgg cgcgctcaag agcgccgccg cctggtccag atcgcggctc tgccgcgcgg
     5821 agccgaacca ctgcggcgcc gcgccgagca gcggcacgct cagccggaag aggaggatcg
     5881 gcacgacgaa gagcgccagc agcgagagca ggagccagcg ccggatgctg agggttctca
     5941 tcgcggctgg ccctcgaagc ggtagccgac gccccagacg ttgacgatta gcctggggcg
     6001 ctcgggcgcg gcctcgatct tctcgcgaat ccgcccgaca tgcacggtga cggtgtgccg
     6061 gtcgccgatg ccctcccagc agcgctccaa gagctggtcg taggtgaaga cctggcgcgg
     6121 atgcgcggcc agcagcgaga gcagctcgaa ctccttgggt gtcagcgaca cggaccggct
     6181 ctcgacccag acctcgcgcg ccgccggatc gagcgccagc cgcccgaagt cgagcagcgc
     6241 cggccgcgct ttcgtcgcgc cgccagagcg ccgcagcacc gccttgacgc gcgccaccac
     6301 ctccgcgggg gtggccgact tgacgatgta gtcgtcgccg ccgaggccca ggccgcggat
     6361 cttgtcgacg tcctcgccgc gggcgctgag gaagaggatc ggcacgtcgc tgctgcggcg
     6421 tagccggcgg cagagctcga agccgctctc gcccggcatc atgacatcga gcaggacgca
     6481 gtcgaccggc tcctgctcca gcagactggc ggcggcggcg gcatcgccgg ccgcgcgcac
     6541 gcggtagcct tcagcctcca ggaaatcgcg catcagctcg acaatggcgg ggtcgtcgtc
     6601 gaccaccagc actgtccgct cctcgttcac caacaccctc cgcccagccg cgacgcgtgg
     6661 cggccgcgtc acggccgcgc cctgagtatg cctgtcagcg gctgccgtag cagcccgcgg
     6721 acccgcgccc tgagtggact gtgcgcggac tgtgagcgtt tcgtgagcgc gtggtctagt
     6781 ggatcgtgac tggcgcggct cagactggag ctgtgccggt gatcgggccg gcacgacggc
     6841 cgatcgaagc caactgatgg gagagtagat gcgcagaggt ctcgcggtaa tcttcgccgc
     6901 gctggcgggg ctggtcctgg tggcggtggt ggcgcagttc tttctggcgg gcgccggtct
     6961 cttcggcgcc gtgagcggct atcagtcaca cgagatcgtc gggatgctga tcgcgacggc
     7021 gtcgatcgtt ctgctgctgc tggccatctt cgggcgcttc gggcgcgcgt tcatcatcca
     7081 cgcggcggcg ctgctggtgt tgctgctggt tcagatcgcg ctgatcgaga gcgaccagcc
     7141 ctggttggag gcgctgcacc cggtcaacgc gctgctgatc ctcggcgcca gcgcgcagtt
     7201 ggcgcagcgc gcgtgggcat gggttcgccg gccaggcccg gtcgccgcca gcgtcgccga
     7261 gcgagtgctg cgtgagagag ggttttgagt catgggactg agtttgggct tgctggccgt
     7321 cgcgggcgat ccggccgtcg gctgggcgca cgcggggcat ggattcgtct gggggccgat
     7381 cgtgttgttc ttctggattg gcgcgctgat catgttcgcc tggttcgtgg cgcgcgggcc
     7441 atggatgcgt ggcgggcagc gtggcccgca cggtgtggag cgggcgcgcg agatcctggc
     7501 cgagcgctac gcccgcggcg agatctcgac cgaggagtat tgggagcggc gggagcagtt
     7561 gttgtagctg gcgtcccgcg tccgggccgc gccgtatact tcggcgtcgc ccgggcgcgg
     7621 cctcgtcccg gcaggcgagg cgcgcagcac gcgctctggc agatggcgcc ggatgatgag
     7681 ggtcaaactg aaccagccag cacgaagcta cctcgcctct ctggtggccg tcgccggcac
     7741 cgtggcgctg ctcctgccgt tccgcgccgc gctcgctacc ctcagcgtcg cgctgatctt
     7801 tctgatcgtg gtcaccgcgg tcgcgctcta cggcaatacc tggtccggcg tactcgcctc
     7861 tttgaccgcg tttctctgct tcgacttcgt cttcatcccg ccctattacc ggctgaacgt
     7921 ggcgcatctc gacgatgtgt tggtgctctt cgtgtttctg gggatcgcgg tgctcaccag
     7981 ccagctggtg gtgcgcgtgc ggcagcagac cgatgaggcg ctacggcgcg ggcacgagac
     8041 caccagcctc tacgacttga gcatcgcgct gatcggcgag tcgagcctgc ccgagatgct
     8101 gacgaccatc gcgcgccaca tggagcgctt gttcgcgctc gacagctgta gcgtcctgct
     8161 cgacgagcgg gtttcaccca gcggatccag cgcgagcgcg gcgcagctgg cagagcagag
     8221 cacgccgcgc accgagcacg ttcccggcac cctggcgatc ccggtcggca cggcccggcg
     8281 cgcgttgggc gtgctcgtgg tctcgcgccg gccgccgcgc gccgtcttcg atctgggaga
     8341 gcggcggctg ctggagacct tcgccaacca ggccgcgatc gccatcgagc ggatcattct
     8401 gaccgaagaa gagacgcgcg cggaggtgct ggccgagtcc gacgcgctca agagcacact
     8461 cctctccgcc gtctcgcacg agctgcgcac accgctggcc tcgatcaagg cctcggcgac
     8521 cagcctgctg caacccggca tcgagtgggc ggaggtggat cgccgcgagc tgcttgaggc
     8581 gatcgacgag gaatcggacc ggctcaaccg tctggtggcc aacctcctcg acctctcgcg
     8641 catcgaagcc ggtgtgctgc ggcccgcctt cgattggtgc gagccgggtg aggtgatcta
     8701 cgaagcggtc aaccgctgcg agacgtcgct gcgtggccac cgcctggccc tcgcgatccc
     8761 cgacgacctg ccgccgctgc ggatcgatt
//
LOCUS       DASUBI010000649         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_58884, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000649 DASUBI010000000
VERSION     DASUBI010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58884"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..458)
                     /locus_tag="VFU72_12560"
     CDS             complement(186..458)
                     /locus_tag="VFU72_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424369.1"
                     /translation="MRRLLVLVLVIAALSVVAVAPASAHVHGITPLRCTPAVQNTDNT
                     GANATDDTPAAAANGGPIVGLIPRDVGSAPLTVGDGGFDAAVCQQQ"
     gene            670..2910
                     /locus_tag="VFU72_12565"
     CDS             670..2910
                     /locus_tag="VFU72_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5424370.1"
                     /translation="MSHGNLYRQSLSRPRGQRRAARFGRAGRVLPRPRPLPPELLHAG
                     PRRRPRQPLLRRTASLGCLALLLGAGLWAGLIAAVAAGGYTAWSSITSTLPTLAQMQQ
                     PSFATTKIYDRNGILLYQVADPNSGWRTPVGYQEILDHIGQQQQSPNAPHHAWIFDAT
                     VAAEDASFWTNHGVDPTAIARSFVEDISGSGFSGASTITQQLVRLVYPDQIGYQVSYS
                     RKLREAIMAYRFSQHFTKPQILEMYLNDIYYGEGAYGIDAASEIYFGEHPWDLSLGQA
                     ALLAGLPQAPTAYDPFDNYQAARARQQYVLAQMVKTGAITSQQADDAYATPITLAPPD
                     VTRPSAPLAPHFVNFVLGWLEQRFGSNAVYGGGLIVKTTLDYHLQQTAQQIVAKQVKQ
                     IAWDDVDNGALVAMLPETGEIVAMVGSADYYNNAIDGQVNVAVMPRSPGSSIKPVTYL
                     AALERGFYPGTILNDTDKKYPIPGQPGKFYEPHNDTFKHYGKVTVREALDNSLNVPAV
                     ETLNYVGVQTMIDLDHALGNKSTMYSGYYGLSVTVGGGDVTLLDHTTVFATLANEGRY
                     VPYTPVLQVNGQGGKLLYQLNRGKAEQDGQQVAPAGNVYQISNILSDNKARTMIFGAN
                     SPLHVPELNRPVAAKTGTSDGPRDGLTMGYTTDLVVGVWTGNTDNSLMGSLDAVSTAG
                     PIWHDFMVAAHQPRFATTLAGPDGKPVPPDFPVPPNVSMGKACDKPTPEVLVRGVKVV
                     CDAGAP"
BASE COUNT          501 a         1113 c          937 g          433 t
ORIGIN      
        1 cctgcttctt cactcgctcc gagggccgcg ccaccaaggg cacacgcctg ctggagctgc
       61 ccagcgacga ccgcgtcagc accatcgcgc ggctggcggc ggagtagcgg cagacttatt
      121 tcactcgcac gaaaaccgcc cgctccacaa tggagcgggc ggttcgcgtt cggcttcgga
      181 tgccgctatt gctgctggca gaccgccgcg tcgaagccgc cgtcgccgac ggtcagcggg
      241 gcgctgccga catcacgcgg gatcagcccg acgataggcc cgccattggc ggccgcggcc
      301 ggcgtgtcgt ccgtcgcgtt ggcgccggtg ttgtccgtgt tctgcacggc cggcgtgcag
      361 cgcagcggcg tgataccgtg gacgtgggcc gaggccggcg ccaccgcgac cacgctcagc
      421 gcggcgatga cgaggaccaa tacgaggagc ctgcgcaaat caccctccta accctgtgcc
      481 tactctccga taccccgtgc ctctcctacg cccaacgcgc gtaaccggat tgctctctcg
      541 cgagcgaggt tcgggcatga tgcgcggccg gctcgtcgtt aacccgtggt tcagaattcg
      601 cgcgtagagt gggtgtggga ggcgctcgcc tcccggcagt ccaatcgcac cgacacgagt
      661 cagcgtccga tgagccacgg caatctctac cgccagagtc tctcgcgccc gcgcggccag
      721 cgccgcgccg cccgcttcgg tcgcgccggc cgggtgctgc cgcgcccgcg gccgctgccg
      781 ccggagcttc tgcacgcggg accccggcgc cggccgcgcc agccgctgct gcggcgcacg
      841 gcctcgctcg gctgcctggc gctgctgctc ggcgccggcc tctgggccgg gctgatcgcc
      901 gcggtcgccg ccggaggcta caccgcctgg tcgagcatca ccagtaccct gccgacgctg
      961 gcgcagatgc agcagccgag cttcgccacc accaagatct acgaccgcaa cggcatcctc
     1021 ctctatcagg tggccgaccc caacagcggc tggcgtaccc cggtcggcta ccaggagatc
     1081 ctcgaccaca tcggccagca gcagcaatca cccaacgcgc cgcaccacgc ctggatcttc
     1141 gacgccaccg tggccgccga ggacgcctct ttctggacca accacggcgt cgaccccacc
     1201 gccatcgccc gcagcttcgt cgaggacatc agcggcagcg gattctccgg cgcctcgacc
     1261 atcacccaac agctcgtgcg gctggtctac cccgatcaga tcggctacca ggtcagctac
     1321 tcgcgcaagc tgcgtgaggc gatcatggcc taccgtttca gccagcactt caccaaaccg
     1381 cagattctgg agatgtacct caacgacatc tactacggcg agggcgccta cggcatcgac
     1441 gccgcctcgg agatctactt cggcgagcac ccctgggatc tctcgctcgg ccaggcggcg
     1501 ctgctggccg ggctacccca ggcgcccacc gcctatgacc ccttcgacaa ttaccaggcg
     1561 gcgcgggcgc gccagcaata cgtgctggcg cagatggtca agaccggcgc catcaccagc
     1621 cagcaggccg acgacgccta cgccaccccg atcaccctgg cgccgcccga cgtcacccgc
     1681 cccagcgccc cgctggcgcc gcacttcgtc aacttcgtgc tcggctggct ggagcagcgc
     1741 ttcggctcca acgcggtcta cggcggcggc ctgatcgtca agaccacgct cgactaccac
     1801 ctgcagcaga cggcgcagca gatcgtggcc aaacaggtca agcagatcgc ctgggacgat
     1861 gtggacaacg gcgcgctggt cgccatgctg ccggagaccg gcgagatcgt ggccatggtc
     1921 ggcagcgccg actactacaa caacgccatc gacggccagg tcaacgtggc ggtcatgccg
     1981 cgctcgcccg gcagctcgat caagccggtg acctacctgg cggcgctgga gcgcggcttc
     2041 tatcccggca cgatcctgaa cgacaccgac aagaagtacc cgatacccgg ccagccgggc
     2101 aagttctacg agccgcacaa cgacaccttc aagcactacg gcaaggtcac cgtgcgcgag
     2161 gccctggaca actcgctcaa cgtgccggcg gtcgagacgc tcaactacgt cggcgtgcag
     2221 acgatgatcg acctcgacca cgcgctcggc aacaagtcga ccatgtacag cggctactat
     2281 gggctctcgg tcacggtcgg cggcggcgat gtgacgctgc tcgaccacac caccgtcttc
     2341 gccacgctgg ccaacgaggg ccgctacgtg ccctacacgc cggtcctaca ggtcaatggg
     2401 cagggcggca agctgctcta ccagctcaac cggggtaagg ccgagcagga cgggcagcag
     2461 gtcgcgcccg ccggcaacgt ctaccagatc tccaatatct tgagcgacaa caaggcgcgc
     2521 accatgatct tcggcgccaa ctcgccgctc cacgtgcccg agctgaaccg gcccgtcgcg
     2581 gccaagaccg gcacctccga cggcccgcgc gacgggctga ccatgggcta caccaccgat
     2641 ctggtggtcg gcgtctggac cggcaacacc gacaactcgc tgatggggag tctcgacgcg
     2701 gtctcgaccg ccggtccgat ctggcacgac ttcatggtgg ccgcgcatca gccgcggttc
     2761 gccacgaccc tcgccgggcc ggacggcaag ccggtgccgc ccgacttccc cgtgccgccc
     2821 aacgtctcta tgggtaaggc ctgcgacaag cccacgcccg aggtgctggt gcgcggcgtc
     2881 aaggtcgtct gcgatgccgg cgcgccgtag gcaatgtcgg aggaatcggg cgactgcgtc
     2941 agcgtgggaa cggcgcggtg gccgtcgagg ggcttgggtg cctt
//
LOCUS       DASUBI010000650         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_204544, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000650 DASUBI010000000
VERSION     DASUBI010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204544"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VFU72_12570"
     CDS             <1..1447
                     /locus_tag="VFU72_12570"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5424371.1"
                     /translation="SATGEIFETYNPATNEVIGRVPKAGTEDVERAVAAARQAFDHGP
                     WATMSPNERAARLRRLAALLRERQEEIARLETTNCGKIIIESRGDVAASANCVDYYAN
                     LTGQIYGETIPMNGPLFDYTLREPVGVCGQIIPWNFPLLMAAWKLAPALATGNTIVLK
                     PASATPITALLLGELCLEAGIPAGVVNVLTGPGAQVGAAIAGHPAVDKIAFTGETETG
                     RQILALAAGTIKKVSLELGGKSPNIVFPDADLDEAVNGSLFAIFTNAGQRCTARTRLF
                     LHESIHDQFMSEFVAKAGKIRIGDPLEDATQMGPVISPRQCERVLSFVELAQGEGAAL
                     LLGGKKPTEDVLKNGNFVEPTIFDQVRNDMTIAQQEVFGPVLSVIPFREESQVVELAN
                     NSIYGLAATIWTNDVKRAHRLAKNLRAGNVSINYPTVNPPEAPFGGYKQSGIGRELGR
                     HAVELYTQVKNVVVNLSEEPFDWYGRWPTGR"
     gene            complement(1669..2223)
                     /locus_tag="VFU72_12575"
     CDS             complement(1669..2223)
                     /locus_tag="VFU72_12575"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEU5424372.1"
                     /translation="MARTEQSTQRPIYLVAVAYDDEFKADEARILVKRAQREGISEVL
                     ETAVVVHDRDGKKLLTQDVDLEGKRRLQGHWLGIAAALATGVQPLIFVGTAAGEIIGR
                     LTDTGIQTSDLRRVGKQLTPGTSALFVLAHEAVPRGALAKRLQPLGGRVIRTTLSPAL
                     NDELEWILRENVPDRASPLPAVSG"
     gene            complement(2235..2738)
                     /locus_tag="VFU72_12580"
     CDS             complement(2235..2738)
                     /locus_tag="VFU72_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEU5424373.1"
                     /translation="MTTNGVQPRITERRAVQTELDRRPAPAEPSAEEPGRGGADINTR
                     LAWLRTQLAVERTLMAWNRTSLSLIGFGFTIYEFLKKVQEASGTNVLRPQAPRNFGLA
                     FVTIGTLGTLVALWQHILYGRYLRGHELKDVAIREGMPHASLPLAITVFLAVVGTVTI
                     AWILVGG"
     gene            complement(2750..4120)
                     /locus_tag="VFU72_12585"
     CDS             complement(2750..4120)
                     /locus_tag="VFU72_12585"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEU5424374.1"
                     /translation="MQEQTTTARDVLAAVPIAEQEARAIGIEAYHYFYPLVTMDVTRR
                     QVTNLPTGIKPGFGPVNHFAHLRAFPEPAFRTVVRPNFDALYSTAWLDLTTEPMIVSA
                     PDTHGRYYLLQLLDMWTDTFAAPGTRTSGTGAQHHAVVPPGWQGQLPAGVERIQAPTP
                     HVWIIGRTQTNGPADYAAVHQIQDGFRLTPLSRWGRQPQTVSFIPDPSVDMRTEPLRQ
                     VNGMTAASFFGYAAELLKLHPAHVTDWSMLARLRRIGFTPGQGFAYEQLPPAVQQALA
                     GVPAAALRTMRAKLPTFGRVVNGWSLMSDTVGVYGDSYLKRACIAMTGLGANQPEDAI
                     YPLNVADADGAPLSGEHEYVLHFAQGELPPVDGFWSVTMYDAEGFPVANPLHRYALGS
                     RDALTSNDDGSLDLYLRHDAPGVERESNWLPAPRGPLGVTMRLYAPKPAALDGTWNPP
                     AVKRLF"
     gene            complement(4301..5740)
                     /locus_tag="VFU72_12590"
     CDS             complement(4301..5740)
                     /locus_tag="VFU72_12590"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEU5424375.1"
                     /translation="MQVKPKMATEIPAAITTPDRVETRLGTLRFFDGLPDAATVQTVY
                     DNLDFQRAVQTFLTALPAASFSALRAGIRQFGPDNQTVLITGSLMDSHTLLLTANTET
                     IYNLAWLDTRNGPLVIEVPPKVLGLIDDFWQRYVGDVGNAGPDKGQGGTYLLLPPGYS
                     GAVPEGYYVARSRTYGNVLGLRALIQDGGVRATTERVKQHLRVYPLAQAADPPPMTFV
                     DISGVAFNTIHANDVSFFDEVVPVVHEEPLEAIEPEARGLLAAVGIQKGKPFAPDARM
                     QHILAEAAAVGNATARALVFQTREPSAHYYPNSAWQMVWLGNDYQFSPDGVLNLDARS
                     LFFYMGIGASPAMAIKMVGLGSQYAMAYHDAAGHYLDGAGSYRLHLPAGIPAKDFWSL
                     LVYDPQTRSMLQTDQQFPSLGSQKPDLVVNPDGSVDVYFGPKPPFGHEANWVQTIPGK
                     GWFVALRLYGPLEPWFDQTWRPGEIEEIS"
     gene            complement(5832..>6234)
                     /locus_tag="VFU72_12595"
     CDS             complement(5832..>6234)
                     /locus_tag="VFU72_12595"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEU5424376.1"
                     /translation="MTLKMVGVGSQYAWSCRDAAGHYLDGGKTYRLRLPAGIPAKDFW
                     SVIVYDPQTRSMLQTDQPFPSISSQKADLVVNPNDSVDVYFGPKPPLGHEANWVQTIP
                     GKGWWTILRLYGPLEPWFDQTWRPGEIELMP"
BASE COUNT          985 a         2092 c         2141 g         1016 t
ORIGIN      
        1 atcggccacg ggcgagatct tcgagaccta caatccggcc accaacgagg taatcgggcg
       61 cgtgcccaag gcgggcacgg aggatgtcga gcgcgcggtg gccgcggcgc ggcaggcctt
      121 cgatcacggc ccctgggcga cgatgtcgcc caacgagcgc gccgcgcgac tgcggcggct
      181 ggccgccctg ctgcgcgagc ggcaggagga gatcgcgcgc ctggagacga ccaactgcgg
      241 caagatcatc atcgagtcgc gcggcgacgt ggccgcctcg gccaactgcg tcgactacta
      301 cgccaacctg accggtcaga tctacggcga gacgatcccg atgaacggcc cgctcttcga
      361 ctacactctg cgcgagccgg tcggggtctg cggccagatc atcccctgga acttcccgct
      421 gctgatggcg gcctggaagc tggcgccggc gctggccacc ggcaacacga tcgtgctcaa
      481 gccggccagc gccacgccga tcacggcgct cctgctcggt gagctgtgcc tggaggcagg
      541 catcccggcc ggtgtggtca acgtgctgac cggccccggc gcgcaggtcg gcgcggccat
      601 cgccggccat ccggcggtcg acaagatcgc cttcaccggc gagaccgaga ccgggcgcca
      661 gatcctggcg ctggcggcgg gtacgatcaa gaaggtgtcg ctggagctgg gcggcaagtc
      721 gccgaacatc gtcttccccg acgccgatct cgatgaggcg gtcaacggct cgctcttcgc
      781 catcttcacc aacgcgggcc agcgctgcac ggcgcgcacg cgcctcttcc tgcacgagtc
      841 gatccacgat cagttcatga gcgagttcgt ggccaaggcg ggcaagatcc gcatcggcga
      901 tccgctggag gacgccaccc agatgggccc ggtcatctcg ccgcgccagt gcgagcgcgt
      961 gctctccttc gtcgagctcg cccaggggga gggcgccgcg ctgctgctgg gcggcaagaa
     1021 gccgaccgag gacgtgctca agaacggcaa cttcgtcgag ccgaccatct tcgaccaggt
     1081 gcgcaacgac atgacgatcg cgcagcagga ggtcttcggc ccggtcctct cggtgattcc
     1141 gttccgcgag gagtcgcagg tcgtcgagct ggccaacaac tcgatctacg gcctggcggc
     1201 gaccatctgg accaacgacg tcaagcgggc gcaccggttg gccaagaacc tgcgcgccgg
     1261 caacgtcagc atcaactacc cgacggtcaa cccgccggag gcgcccttcg gcggctacaa
     1321 gcagagcggc atcgggcgcg agctgggccg gcacgccgtc gagctctaca cccaggtcaa
     1381 gaacgtggtg gtgaatctct cggaggagcc gttcgactgg tacggccgct ggccgaccgg
     1441 gcgctaggag agctcgggcg actcacgcgc cggctcgcgg tcgacacttc gtcgtgattc
     1501 cgaagggcag acctctcctg acgaagtcgg atgggtccgc ccttcgcgcc tcgctccaga
     1561 tgcagtgggt accacgggcc ggattgtcat tccccaccca gggaccgttg gccggggtgc
     1621 cactggtcgt cgatcacagc ctcgcggcgg cgtacgccgc cggcatagct agccggacac
     1681 agccggcagc ggcgaggctc gatcgggtac gttctcccgc aggatccatt cgagctcgtc
     1741 gttcagggct ggcgagagcg tggtgcgaat cacgcgcccg ccgagcggct gtagccgctt
     1801 ggcgagtgcc ccgcgcggca ccgcctcgtg cgccagcacg aacagggcgg aggttccggg
     1861 cgtcaattgc ttgcccaccc gccgcaggtc actggtctgg atccccgtat cggtcagccg
     1921 cccgatgatc tccccggcgg ccgtgcccac gaagatcagc ggctgcacgc ccgtcgccag
     1981 cgccgcggcg atgcccagcc aatgaccctg caaccggcgc ttgccctcga gatcgacatc
     2041 ctgggtgaga agcttcttgc cgtcgcggtc gtgtacgacg accgcggtct ccaagacctc
     2101 ggatatcccc tcgcgctgcg cccgtttgac caggattcgc gcctcatccg ccttgaactc
     2161 gtcgtcgtag gcgaccgcga cgaggtagat cggtcgctgc gtcgattgtt cagttcgtgc
     2221 catgaccttc ctgcctaccc tcccaccagg atccaggcga tcgtcaccgt cccgaccacg
     2281 gccaggaaga ccgtgatggc cagcggcagc gaggcgtgcg gcatcccctc acggatcgcg
     2341 acatccttga gttcgtggcc gcgcagatac ctcccgtaca gaatgtgctg ccagagcgcc
     2401 accagcgtgc cgagagtgcc gatcgtcacg aacgccaatc cgaagttgcg cggggcctgc
     2461 gggcgcagga cattcgttcc cgaggcctcc tgcaccttct tgaggaactc gtagatcgtg
     2521 aagccgaagc cgatcaacga cagcgaggtc cggttccagg ccatcagcgt gcgctcgacc
     2581 gccagctggg tgcgtagcca ggcgagacgg gtgttgatgt ccgcgccgcc ccggccgggc
     2641 tcctccgcgc tgggctctgc cggcgcgggg cgccggtcca gctctgtctg cacggctcgc
     2701 ctctcggtaa ttctcggttg cacgccgttc gtggtcatcc ccgacctcct caaaacaatc
     2761 gcttgaccgc cggcggattc caggtgccgt ccagagccgc cggcttcggc gcgtacagcc
     2821 gcatcgtgac gccgagcggg ccgcgcgggg ccggcagcca gttcgactcc ctttcgacac
     2881 ccggcgcgtc gtgccgcagg tagaggtcga gtgagccgtc gtcgttggag gtcagcgcat
     2941 cccggctccc gagggcgtag cggtggagtg ggttcgcgac cggaaagccc tccgcgtcgt
     3001 acatcgtcac cgaccagaag ccgtcgaccg gcggcagttc accctgggcg aagtggagta
     3061 catactcgtg ctcaccgctg agcggcgcgc cgtcggcgtc ggcgacattg agcgggtaga
     3121 tcgcatcctc cggctggttg gcgccgaggc cggtcatcgc gatgcacgcc cgtttgaggt
     3181 aggagtcgcc gtagacgccg accgtatcgc tcatcagcga ccagccgttg acgacgcggc
     3241 cgaaagtcgg cagcttggcc cgcatcgtcc gcagcgccgc ggcgggaacg cccgcgagcg
     3301 cctgctgcac ggcgggcggc aattgctcgt aggcgaaacc ctggccgggc gtgaagccga
     3361 tccgccgtag ccgtgccagc atcgaccaat cggtaacatg cgcggggtgc agcttcagca
     3421 gctcggcggc gtagccgaag aagctggcgg ccgtcatgcc gttcacctgc cgcagcggct
     3481 cggtccgcat gtccaccgac gggtccggta tgaagctgac cgtctggggc tgccgccccc
     3541 atcgcgagag cggcgttagg cggaagccgt cctggatctg atggacggcc gcgtaatcgg
     3601 ccgggccatt ggtctgcgtg cggccgatga tccagacgtg cggtgtgggc gcctgaatcc
     3661 tctcgacacc ggcggggagt tgcccctgcc agcccggcgg caccacggcg tggtgctgag
     3721 cccccgtgcc actggtgcgc gtgcccgggg cggcgaaggt atcggtccac atgtccagca
     3781 actgcagcag gtagtagcgc ccgtgcgtgt ccggcgcgga gacgatcatc ggctcggtgg
     3841 tcaggtcaag ccaggcggtg gagtagagcg catcgaaatt cggacgcacc accgttctga
     3901 acgcgggctc ggggaaggct ctcaggtgcg cgaagtgatt gaccggcccg aagcctggct
     3961 tgatgccggt tggcagattg gtcacctggc gccgcgtgac gtccatcgtc accagcgggt
     4021 aaaagtagtg ataggcctca atgccgatgg cccgcgcctc ctgctcggca atcggaaccg
     4081 ctgcgagcac gtcacgcgcc gtcgtggtct gctcttgcat cgcaggctct ccctccgccg
     4141 cgccagcccc cggcgcggac cctgctcgtt cgatcatccg ttcaccttcc ggccggggca
     4201 ccaagccagc tggccggctg gatgcccgcc gcgtggcggg catccagctc tcgacgtggc
     4261 cgtccggcac gctcgcgccg gacacgcatc catcccagga tcacgagatc tcctcgatct
     4321 cgcccgggcg ccaagtctga tcgaaccagg gctccagcgg gccgtagagg cgcagagcca
     4381 cgaaccagcc cttacccggg atggtttgca cccagttggc ttcgtggccg aatggcggct
     4441 tggggccaaa atagacatcc accgagccgt ccgggttgac gacgaggtcc ggcttctggc
     4501 tgccgagact ggggaactgc tggtcggtct gcagcatcga gcgcgtctgc ggatcgtaga
     4561 ccaggagcga ccagaagtcc ttggccggga tgccggccgg caggtgcagc cggtagctcc
     4621 cggcgccgtc gaggtagtgg ccggctgcgt cgtgataggc catggcgtac tgggatccga
     4681 ggcccaccat ctttatcgcc atggccggac tggccccgat gcccatgtag aagaacaacg
     4741 accgcgcgtc caggttgagc acgccgtcag gcgagaactg gtagtcgttg ccgagccaga
     4801 ccatttgcca ggcgctgttc ggatagtagt gggcgctcgg ctcgcgtgtc tggaagacga
     4861 gagcccgcgc ggtggcgttg ccgaccgccg ccgcttcggc caggatgtgc tgcatgcgcg
     4921 cgtcgggtgc gaagggtttg cctttctgga tgccgacggc ggccagcaga ccgcgcgcct
     4981 cgggctcgat agcctccagc ggttcctcat ggaccaccgg cacgacctcg tcgaagaagg
     5041 agacgtcgtt ggcgtggatg gtattgaacg ccacgccgga gatgtcgacg aaggtcatcg
     5101 ggggcgggtc cgccgcctgg gccagcggat agacgcggag gtgctgcttg acgcgctcgg
     5161 tcgtggcgcg cacgcccccg tcctggatga gggcgcgaag gccgagtacg ttcccgtacg
     5221 tgcgtgagcg cgcgacatag tacccctcgg gcacggcgcc gctgtagcca ggtggcagca
     5281 ggagatacgt ccccccctga cccttatccg ggccggcatt gcccacgtcg cctacgtagc
     5341 gctgccagaa gtcgtcgatg aggccgagca ccttcggcgg gacctcgatg accagtggcc
     5401 cgttcctcgt gtcgagccag gcaaggttgt agatggtctc ggtgttggcg gtgaggagca
     5461 gcgtgtgaga gtccatcagc gacccggtga tgagcacggt ctggttgtcg gggccgaatt
     5521 gccggatccc cgcgcgcaag gcggagaacg aggccgccgg cagggcggtt aggaacgtct
     5581 gcacggcccg ctggaagtcg aggttgtcgt agacggtctg cacggtcgcc gcgtcgggca
     5641 gaccgtcgaa gaacctgagt gtgcccaggc gcgtctcgac ccggtcgggg gtggtgatgg
     5701 ccgccggaat ctcggtcgcc atcttgggct tgacttgcat ttcggtcact cctttcgctc
     5761 cgtcacgcgt ggttggtcac gacggtgacg gaggccgacc cgttgcactg gatcgccctc
     5821 cgtcggctgc ctcacggcat cagctcgatc tcgcccggcc gccaggtctg gtcgaaccag
     5881 ggttccagcg ggccgtagag gcgcaggatg gtccaccagc ctttgccggg aatggtttgc
     5941 acccagttgg cttcgtgccc aagtggcggc ttcgggccga agtagacgtc caccgagtcg
     6001 ttggggttga cgaccagatc agccttctgg ctgctgatac tcgggaacgg ctggtcggtc
     6061 tgcagcatcg agcgcgtctg cgggtcatag acgatcaccg accagaagtc cttggccggg
     6121 atgccggccg gcaggcgcag ccggtaggtc ttgccgccgt cgaggtagtg gccggcggcg
     6181 tcgcggcagc tccaggcgta ctgggagccg acaccgacca tcttcagggt catc
//
LOCUS       DASUBI010000651         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1216183, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000651 DASUBI010000000
VERSION     DASUBI010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216183"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFU72_12600"
     CDS             complement(<1..206)
                     /locus_tag="VFU72_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424377.1"
                     /translation="MAWRQVLDLYDVLDSAEVDGEQVAALLRAGGVADVTVQRAQGER
                     GYTDIVKAVIPGKRGKLAGGEAPTL"
     gene            complement(216..962)
                     /gene="pgl"
                     /locus_tag="VFU72_12605"
     CDS             complement(216..962)
                     /gene="pgl"
                     /locus_tag="VFU72_12605"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5424378.1"
                     /translation="MTQPEIRVVSDGAALAELAARLFVEAAAASIAARDDFAVALSGG
                     STPRAMFQRLAQTPLREQVDWSKLHVFWGDERTVPPDSPESNYHMARQALLAHVPVPD
                     DQIHRMQGELDPYRAAADYEAVLRQVFQLGQQPRLPVFDLQLLGIGSDGHTASLFPGT
                     PALHERGRLCVANPVPQLQTTRLTLTPPVITASALVLVLAAGAEKAEAVARAIQGEWD
                     FDTTPSQLLRHSEGRVVWLLDPEAAADLTQ"
     gene            complement(959..2152)
                     /locus_tag="VFU72_12610"
     CDS             complement(959..2152)
                     /locus_tag="VFU72_12610"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEU5424379.1"
                     /translation="MPEIDLSAQVRGFSGPAIDLAAIERDLTALWRMPTLAGLRQSEV
                     VPTRTSVLNLVMIAGSKELARRGTRVIGQLATHHPSRAIIFATSTDPADFAGDIDARV
                     STHCYAGSGQHFAACFEQIEITVPRDALDYLTSLIMPLTLPDLPTYLWYPGQPPLADR
                     RFARLARVADRLILDSLEFPRGGRFLARAAELARQDDGCPSLADLNWGRLIPWRRALA
                     HFFDLPECRWALDHLVSARVELGASNTRRTEPNEAQGLLYLGWLMAQLGWQPLSAEAP
                     EGGWRFLARDGRDQVVDLELTARPAPRTLDGALTAVRLEAQDGSRQARFELNRVGGDL
                     ATIRTVVSNGGRQSFEHAMQAPCLDTSKLLGRELEEPRRDELFVEALGEASRLLALRQ
                     KRSHA"
     gene            complement(2182..3723)
                     /gene="zwf"
                     /locus_tag="VFU72_12615"
     CDS             complement(2182..3723)
                     /gene="zwf"
                     /locus_tag="VFU72_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5424380.1"
                     /translation="MQVVTLQNPLREGLDVERVPPPSAMVIFGASGDLTMRKLMPALY
                     NLAVEGLLPPGFGMVGYARRPYTTEEFRQHMREAVDQFSRRRPVDPEVWQSFEQGLFY
                     VPGTFESPEGYHQLDAQLQQLDKERGTAGNRVYYLATPPDAYEEIVNNLGHGGLVNTN
                     AESFYRLIVEKPFGFDLKSAIALNDLLQTVFREDQIYRIDHYLGKETVQNIMAFRFAN
                     GIFEPIWNRNYIDNVQITVAESIGIEGRGGYYDETGALRDMAQNHLLQLLSVIAMEPP
                     IAFEANTVRDEKVKALKAIRPIDPAEVDQYTVRGQYGPGWVAGHPVPGYREEPKVNPE
                     SLTETYVALKLIIDNWRWAGVPFYLRTGKRLPRRVSEIAIEFKQVPHPLFPPSVIEQM
                     EPNVLAIRIQPDEGVSLKIAAKVPGPRLRLRSVNMGFLYGTAFFVESPDAYERLLLDA
                     MKGDSTLFIRRDETEAAWRPITRILEGWSEAPPPSFPNYEAGTWGPAAADAFIERDGR
                     RWRRP"
     gene            complement(3756..>4147)
                     /locus_tag="VFU72_12620"
     CDS             complement(3756..>4147)
                     /locus_tag="VFU72_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HEU5424381.1"
                     /translation="GIEYGMMEAYAEGFEIMHEAPFDYDLAKLAGLWDQGSVIRSWLL
                     ELAERAFRADPGLERLRGYVEDSGEGRWTVAQAIEENVPAPVITLSLLVRLRSRQEES
                     FGNKVLAALRHEFGGHAVQEEIGQVHG"
BASE COUNT          583 a         1406 c         1502 g          656 t
ORIGIN      
        1 agcgtcggcg cctcgccgcc cgccagcttg ccccgtttgc cggggatcac cgccttgacg
       61 atgtcggtgt agccgcgctc gccctgcgct cgctgcacgg tgacatccgc cacgccgccg
      121 gcgcgcagca gcgccgccac ctgctcgccg tcgacctcgg cgctgtcgag cacgtcgtag
      181 agatcgagca cctgccgcca ggccatcgcc gctccctact gcgtcagatc tgccgccgcc
      241 tcagggtcga gcaaccagac gacccgtccc tcgctgtggc gcaggagttg ggagggggtg
      301 gtgtcgaagt cccactcgcc ctggatagcc cgcgccaccg cctcggcctt ctcggcgccc
      361 gccgccagca ccaggaccag cgccgaggcg gtgatgaccg gcggcgtcag cgtcaggcgg
      421 gtggtctgca attggggcac cgggttggct acgcagagcc gccctcgctc gtgcagcgcc
      481 ggcgtgcctg ggaagagcga ggcagtgtgg ccgtccgacc cgatgccgag cagctgcagg
      541 tcgaagacgg gcagacgcgg ctgctggccg agctggaaga cctgccgcag cacggcctcg
      601 tagtcggcgg ctgcccggta ggggtccagc tcgccttgca tgcggtggat ctggtcatcg
      661 gggaccggca cgtgcgccag cagcgcctgg cgcgccatgt ggtagttgct ctcggggctg
      721 tcgggcggca cggtgcgctc gtctccccag aagacgtgta gcttcgacca gtcgacctgc
      781 tcgcgcagtg gggtctgggc cagccgctgg aacatggcgc gcggggtcga gccgccggag
      841 agcgccacgg cgaagtcgtc acgcgccgcg atgctggccg cggcggcctc gacgaagagc
      901 cgcgccgcga gctcggccag cgcggcaccg tccgatacaa cgcggatctc gggctgggtc
      961 atgcgtggct ccgcttctgc cgcagggcca gcaggcggct ggcctcccct agcgcctcga
     1021 cgaagagctc gtcgcggcgc ggctcctcca gctcgcgccc caggagctta ctcgtgtcga
     1081 ggcagggcgc ctgcatggcg tgctcgaagc tctgccggcc gccgttgctg acgacggtac
     1141 gaatggtcgc cagatcgcca cccacccggt tcagctcgaa gcgtgcctgg cgcgagccgt
     1201 cctgcgcctc cagccgcacc gcggtcagtg cgccgtcgag cgtgcgcggc gccggtcggg
     1261 ctgtcagttc caggtcgacg acctgatcgc gtccatcgcg cgccaggaag cgccagccgc
     1321 cctcgggcgc ctcggcgctg agcggctgcc agccgagctg ggccatcagc cagccgaggt
     1381 agagcagccc ctgcgcctcg ttgggctccg tgcggcgtgt gttgctcgcg ccgagctcga
     1441 cgcgggcgga gaccaggtgg tcgagtgccc agcggcactc cggcaggtcg aagaagtggg
     1501 ccagcgcgcg gcgccagggg atcagccggc cccagttgag gtcggccagc gacgggcagc
     1561 cgtcgtcctg gcgcgccagc tcggcggcgc gtgccaggaa acgcccgccg cgcgggaact
     1621 ccagcgagtc gaggatcagc cgatcggcca cccgcgccag gcgcgcgaag cgacgatccg
     1681 ccagcggcgg ctggccagga taccagagat aggtcggtag gtcagggagc gtcagcggca
     1741 tgatcaggct ggtgagatag tcgagcgcgt cgcggggcac ggtgatctcg atctgctcga
     1801 agcaggcggc gaagtgctgg cccgagccgg cgtagcaatg ggtgctgacg cgggcgtcga
     1861 tgtcgccggc gaagtcggcg gggtcggtgc tggtggcgaa gataatggcg cgtgaggggt
     1921 gatgagtggc gagctggccg atgacccgcg tgccgcgccg ggccagctcc ttgctgccgg
     1981 cgatcatgac caggttgagg acgctggtgc gggtcggcac tacttccgac tggcgcagcc
     2041 cggccagcgt cggcatgcgc cagagcgcgg tcaggtcgcg ctcgatggcc gccaggtcga
     2101 tggcgggtcc gctgaagccg cgcacctgcg cgctcagatc gatctccggc atgtccacca
     2161 ctcctttgtg gttgacgccc gctacgggcg gcgccagcgc cggccgtcgc gctcgatgaa
     2221 ggcgtccgcc gcggccggac cccaggtgcc ggcctcgtag ttcgggaagc tgggcggggg
     2281 cgcctcgctc cagccctcca ggatgcgcgt gatcgggcgc caggcggcct cggtctcgtc
     2341 gcgccggatg aagagggtcg aatcgccctt catcgcatcc agcagcagcc gctcgtaggc
     2401 gtcgggcgac tcgacgaaga aggcggtgcc gtagaggaac cccatgttga ccgagcgcag
     2461 ccgcagccgc ggccccggca ctttggcggc gatcttcagc gagacgccct cgtccggctg
     2521 aatccggatc gccagcacat taggttccat ctgctcgatg accgacggcg gaaagagcgg
     2581 gtgcggcacc tgcttgaact cgatggcgat ctcgctcacc cgccggggca ggcgcttgcc
     2641 ggtccgcagg tagaagggta cccccgccca gcgccagttg tcgatgatca gtttcagcgc
     2701 cacgtaggtc tcggtcagcg actcggggtt gaccttcggc tcctcgcgat agccggggac
     2761 cgggtggccg gccacccagc ccggcccgta ctggccgcgc acggtgtact ggtcgacctc
     2821 ggccgggtcg atcggccgga tcgccttgag cgccttgacc ttctcgtcgc gcaccgtgtt
     2881 ggcctcgaag gcgatcggcg gctccatcgc gatcacgctg aggagctgga gcaggtggtt
     2941 ctgcgccatg tcgcgcaggg cgccggtctc gtcgtagtag ccgccgcgac cttcgatgcc
     3001 gatgctctcc gccacggtga tctggacgtt gtcgatgtag ttgcggttcc agatcggctc
     3061 gaagatgccg ttggcgaagc ggaaggccat gatgttctgc acggtctctt tgccgaggta
     3121 gtggtcgatg cggtagatct gatcctcgcg gaagaccgtc tgcaggagat cgttcagcgc
     3181 gatggcgctc ttgagatcga agccgaaggg cttctccacg atcaggcggt agaagctctc
     3241 cgcgttggtg ttgaccaggc ccccgtggcc cagattgttg acgatctcct cgtaggcgtc
     3301 gggcggggtg gccaggtagt agacgcggtt gccggccgtg ccgcgctcct tgtcgagctg
     3361 ctggagctgc gcgtcgagct ggtggtagcc ctcggggctc tcgaaggtgc cggggacgta
     3421 gaagagcccc tgctcgaagc tctgccagac ctcgggatcg accggccggc gacgtgagaa
     3481 ctggtcgacc gcctcgcgca tgtgctggcg gaactcctcg gtggtatagg ggcggcgcgc
     3541 gtagccgacc atgccgaagc cgggcggcag cagcccctcg accgccaggt tgtagagcgc
     3601 gggcatcagc ttgcgcatgg tcaggtcgcc cgaggcgccg aagatgacca tggccgaggg
     3661 gggcgggacg cgctcgacgt cgagtccctc ccgtagcggg ttttgcagcg tgacgacctg
     3721 catgcattcc tctccgtggc ggctatgtgc cggctctagc cgtggacctg gccgatctcc
     3781 tcctggaccg cgtgcccgcc gaactcgtgg cgcagcgcgg ccagcacctt gttgccgaac
     3841 gactcctcct gacgtgagcg cagccgcacc agcagcgaga gtgtgatgac cggggccggc
     3901 acgttctcct cgatcgcctg tgccaccgtc cagcgtccct cgccggagtc ctcgacgtag
     3961 cctcgcaggc gctccaggcc ggggtcggcc cggaaggcgc gctcggccag ctccagcagc
     4021 caggagcgga tcacgctgcc ctggtcccag aggccggcga gtttggccag gtcgtagtcg
     4081 aagggcgcct cgtgcatgat ctcgaagccc tcggcgtagg cctccatcat gccgtactcg
     4141 atgccgt
//
LOCUS       DASUBI010000652         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_292481, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000652 DASUBI010000000
VERSION     DASUBI010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292481"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFU72_12625"
     CDS             <1..370
                     /locus_tag="VFU72_12625"
                     /inference="COORDINATES: protein motif:HMM:NF039174.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424382.1"
                     /translation="YLVGDLGLAAQATRGASKGAKKAAAVDAENAAAATALDEPQPTQ
                     RRRRAKATLIGRGYEFMDLEPEPTVTASDGGSGPLGFAGSGPREGGAEPSGLSTLTRD
                     QFGGASTVPMLPTTWNPDLD"
     gene            407..1186
                     /locus_tag="VFU72_12630"
     CDS             407..1186
                     /locus_tag="VFU72_12630"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEU5424383.1"
                     /translation="MVDFALRSCGLRGHATFAPDEPQLRERLTVDTPAGEAWRCLRCE
                     TFVVGPPRRRGPANTAPEIPRGRFLRDRTLMRALAIERAFRAVLFLLVAAGVVKFSGS
                     RERWQHVLDKDMPLLKPLANQIGWDPEHSRLVHQVTHAFALSSSTLQWIAIGLAGYAL
                     IELVEAVGLWLMQRWGEYFAVIATSVFLPLEIYELTEKITALRLFALVVNIVAVIWLL
                     WSKRLFGLNGGGKAYRAEHEAESLLSVESAALSGTAAGATA"
     gene            complement(1187..>2888)
                     /locus_tag="VFU72_12635"
     CDS             complement(1187..>2888)
                     /locus_tag="VFU72_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424384.1"
                     /translation="IADFDRIELHRRALAALTELPDDPNMLAALAFHADEAGENDAAV
                     RYGIAAAERAASLGANREAASLYALALRHADAVGDNERVGWLERHAFTCYLSGEAEPA
                     VASLRQAITLRRKLADHLAEGDDLRWLSRLLQPLGRAAEAIDAARASLRLLEGLDPSP
                     QLAWSLINMAHICALSLDPACAEYAARAHGLGRELHDAGVDIRARGYTALTAVFSGGT
                     GWGELEAVWREALATPGLEEHAAVLGVLIYWYAVLRCELGRAEGYLAEALTFCDDHDL
                     GMFSSLLHAAATLAALHRGDWDSAAGDAEQILTRPELSPQHRVLPSVTLTLLRARRGQ
                     ASVAPLALEVDNSARPGELVHLGVVWAARAEVAWLAGDDERAIAEARTGIAMAHQHAD
                     PWLTGSLRRWIHLAGGTIDAGPDGALTPFDLEIDGQWRKAADQWTDRGCSYDAALAEL
                     GGDISAVTSALETFRRLGAKAAVRRAQQRLAALRERAPRTRQADKAADPHALTGRQRQ
                     VFDLLAAGLSNPEIAGELHISPKTVGHHVEAILAKLGVQNRTQAVAYALQHQAVGPPE
                     "
BASE COUNT          443 a         1011 c         1000 g          434 t
ORIGIN      
        1 gtacctggtc ggcgacctgg gcctggccgc gcaggccacc aggggcgcca gcaagggcgc
       61 caagaaggca gcggcggtcg acgccgaaaa cgccgcggct gcaacagctc tcgacgaacc
      121 acagcctact cagcgccgac ggcgggcaaa ggccacgttg atcggccgcg gctacgaatt
      181 catggacctg gagccggagc cgacggtgac ggcctcggat ggcggttccg gacccctcgg
      241 tttcgccggc agcgggccgc gagagggcgg ggccgagccg tccgggctgt ccacgttgac
      301 gcgggaccag ttcggcggcg cctcgaccgt gccgatgctg ccgacaacct ggaacccgga
      361 tctcgactga ggccgacgct cggccgtgcc gctgtagcgt gcacccatgg tggattttgc
      421 tctgcgctcg tgcgggttgc gtgggcacgc gacatttgct cccgatgagc cgcagctccg
      481 cgagcgcctg acggtcgaca caccggccgg cgaggcctgg cgctgcctgc gctgcgagac
      541 tttcgtcgtc ggcccgcccc ggcgccgcgg accggcgaac accgcaccgg aaattccgcg
      601 cgggcggttc ctgcgggacc gaaccctgat gcgggcgctc gccatcgagc gcgccttccg
      661 cgccgtcctt tttcttctgg tggccgcggg cgtggtgaag ttcagcggat cgcgcgaacg
      721 ctggcagcac gtgctggaca aggacatgcc cctgttgaag ccgctggcga accagatcgg
      781 ctgggatccg gagcattcca ggctcgttca ccaggtcacc catgccttcg ccttgtcgtc
      841 gtcgacgctg caatggatcg cgatcgggct ggccgggtac gcgctgatcg agctggtcga
      901 ggcggtcgga ctctggctga tgcagcgctg gggcgagtac ttcgcggtga tcgccaccag
      961 cgtcttcctg ccgctggaga tctacgagct aaccgaaaag atcacggcgc taagactttt
     1021 cgcgctggtg gtcaacattg tcgcggtgat ctggctgctg tggagcaagc ggctgttcgg
     1081 gctcaacggc ggcggcaagg cctaccgggc cgagcacgag gcagaaagcc tgctcagcgt
     1141 cgagagcgcg gcgctcagcg gaaccgcagc cggcgcaacg gcgtaactac tccggcggcc
     1201 cgacggcctg gtgctgcagc gcgtaggcga ccgcctgggt gcggttctgc acgcccaact
     1261 tggccaggat cgcctcgaca tggtgaccaa ccgttttggg gctgatgtgc aattcgccgg
     1321 cgatctccgg gttgctcagc ccggcggcaa gcagatcgaa gacctggcgt tgccgcccgg
     1381 tcagcgcgtg cgggtcggcc gccttgtccg cctgccgagt gcggggtgcc cgctcgcgca
     1441 acgcggccag ccgttgctgc gcgcgacgga cggcggcctt ggcgccgagc cgacggaacg
     1501 tctccagcgc cgacgtcacc gcggaaatgt cgccgccgag ctcggccagc gccgcgtcgt
     1561 aggagcagcc gcggtcggtc cattggtcgg cggccttgcg ccactgcccg tcgatctcga
     1621 ggtcgaacgg ggtcagcgcg ccgtccgggc cggcgtcaat tgtcccgccc gccagatgaa
     1681 tccagcggcg cagactgccg gtcagccagg ggtcggcgtg ctgatgcgcc atcgcgatac
     1741 cggtgcgggc ttcggcaatg gcccgctcat catcgccggc cagccaggcc acttcggcgc
     1801 gcgccgccca caccacaccg aggtggacca gttcaccggg ccgggcactg ttgtcgactt
     1861 cgagcgccag cggagctacc gacgcctggc cacgccgcgc ccgtagcagg gtcagcgtca
     1921 ccgacggcag aacccgatgc tgcgggctga gctcgggccg ggtcaggatc tgctcggcgt
     1981 cgccggcggc ggagtcccaa tcgccacgat gcaaggcggc cagtgtggcc gcggcgtgca
     2041 gcaatgagct gaacatgccg aggtcgtggt cgtcgcagaa tgtcagcgcc tcggccagat
     2101 agccctcggc gcggcccaat tcgcagcgca gcaccgcata ccagtagatc agcacaccca
     2161 gaaccgcggc atgttcctcg agccccgggg tcgccagcgc ttcgcgccac accgcttcca
     2221 attcgcccca accggtgccg ccgctgaaca cggcggtgag cgccgtgtag ccgcgggctc
     2281 tgatatcgac tcccgcatcg tgcaattcgc ggcccagccc atgagcccgg gcggcatact
     2341 cggcgcacgc cgggtccaag gagagcgcgc agatgtgcgc catgttgatc agcgaccagg
     2401 ccagttgcgg cgacgggtcg agtccttcca gtagccgcag cgacgcgcgc gccgcgtcga
     2461 tggcttcggc cgcacgtccc agcggctgca gcagacgcga cagccagcga aggtcgtcgc
     2521 cctcggcgag atggtcagcg agcttgcggc gcagcgtgat cgcctgccgc aacgacgcca
     2581 ccgccggctc cgcctcacca ctcaggtaac acgtgaaggc gtgccgctcc agccagccga
     2641 cgcgttcgtt gtcgcccacc gcatcggcat gacgcaatgc gagcgcgtac aggctcgcgg
     2701 cctcccggtt ggcgcccagc gatgcggccc gctccgccgc ggcgatgccg tagcggaccg
     2761 ccgcatcgtt ttcgccggcc tcgtcagcgt gaaatgccaa tgccgccaac atgttcggat
     2821 catcaggcag ttcggtcagc gcggccagcg cgcgccggtg caattcgatg cgatcgaagt
     2881 ccgcgatc
//
LOCUS       DASUBI010000653         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_21450, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000653 DASUBI010000000
VERSION     DASUBI010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21450"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..481
                     /locus_tag="VFU72_12640"
     CDS             158..481
                     /locus_tag="VFU72_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424385.1"
                     /translation="MADPPTTEHAWAFWVFDRPLHRDWLVLAGLAAGVAFAVWTLVRP
                     GRPPALVLAYELFWALPGGVMAAGVVGGTIRELFRTLRRARDQDRDQDRERSQRSGRS
                     ERSNR"
     gene            complement(510..941)
                     /locus_tag="VFU72_12645"
     CDS             complement(510..941)
                     /locus_tag="VFU72_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5424386.1"
                     /translation="MASTNVETYRAGHEAFNRRDFEAMTRRYADRIAWTDHSQGRTFT
                     TPQEFRDDFLPGWVQASSDIRITDPSYLDAGDTVVARFTCKGTHDGPLGPFPASGRAF
                     ALPLCELWHFDASGRVVGGDLYYDQVSLLTQLGQIPQPSSA"
     gene            1094..1858
                     /locus_tag="VFU72_12650"
     CDS             1094..1858
                     /locus_tag="VFU72_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5424387.1"
                     /translation="MDQWTKARIQLCGRFVVDVDGSRIEDTLPGRRGRALFAYLVLNR
                     GRLLPRDELLMAGWGEDASAAAGNALSVLLSKLRHGLGADRLLGRAEIELLLPQATFV
                     DVEAALAGAHRAESAIAEGRWAAAWGPAGIAYHVATRPFLAGLEAPWIDQWRRRLEEV
                     RLRGLECFAAAGLGLGGPALAQAEERARMLTALAPYRETAHRILMEALARRGNVAEAL
                     RAYERLRVLLREELGIAPSPAVQAVHRRLLLQSADS"
     gene            complement(1865..2023)
                     /locus_tag="VFU72_12655"
     CDS             complement(1865..2023)
                     /locus_tag="VFU72_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424388.1"
                     /translation="MALRIVCMCGYVIQGDDDDELWHNAQGHMGVLHPELVGNVTRDD
                     ILAQAEQL"
BASE COUNT          280 a          777 c          798 g          324 t
ORIGIN      
        1 ggccgctgcg gctcgtcggc agccagccga cgaccacggg cgcgatcatc gcgacctacg
       61 ttccagccga ggcctgacca cggccgccgg acctcccgag agcgctgagc ggcgctgacg
      121 agcgccgagc gcggcgttac gatcggcgcg atgcgcgatg gctgatcctc cgaccactga
      181 gcacgcctgg gccttctggg tgttcgaccg gccgctgcac cgcgactggc tggtcctcgc
      241 cgggctggcc gccggggtcg ccttcgcggt ctggacgctg gtgcggcccg gccgcccgcc
      301 cgcgctggtg ctggcctacg agctgttctg ggcgctgccg ggcggggtga tggccgccgg
      361 ggtggtcggc ggcaccatcc gcgagctgtt ccgaacgctg cggcgcgccc gcgaccagga
      421 ccgcgaccag gatcgcgagc gctcgcagcg ttcgggaagg tcggagcgct cgaaccgctg
      481 agcagcgttc gatgcgttcg ccaacccgcc tacgcgctgg acggctgcgg gatctggccc
      541 agctgcgtga gcagcgagac ctggtcgtag tagaggtccc caccgacgac ccgcccgctc
      601 gcgtcgaagt gccacagctc gcacagcggc agcgcgaacg ccctgccgct ggcggggaac
      661 ggtcccagcg gcccgtcgtg tgtgcccttg caggtgaagc gcgccaccac ggtgtcgccg
      721 gcgtcgaggt agctggggtc ggtgatgcgg atgtcgctgg aggcctggac ccagcccggc
      781 aggaagtcgt ccctgaactc ctggggcgtg gtgaaggtcc ggccctgcga gtggtcggtc
      841 caggcgatgc ggtcggcgta tcgcctggtc atggcctcga agtcccgccg gttgaaggcc
      901 tcgtgaccgg cgcggtaggt ctccacgttg gtggacgcca tcgctcctcc tccttggtgg
      961 tcgagcttgc gatggcgtga tcgtggctgc cgcggcttgc gccaaccctt cgccggcctt
     1021 tccccgacct ttccctccgt atgcttgccg tcatcagggg aggacaagga aggatgtcgg
     1081 ctaagctcgc gtgatggacc agtggaccaa ggccaggatc cagctctgcg gccggttcgt
     1141 cgtcgacgtc gacggctccc gcatcgagga cacgctgccc gggcgcagag ggcgagcgct
     1201 gttcgcctac ctcgtcctca accgtggccg cctgctgccc cgcgatgagc tgctgatggc
     1261 tggctgggga gaggatgcct ccgcggcggc gggcaacgcg ctcagcgtgc tgctgtcaaa
     1321 gctgcggcac ggccttggcg ccgaccggct gctgggacgg gcggagatcg agctgctcct
     1381 gccgcaggcg accttcgtcg acgtggaggc ggccctggca ggcgcgcacc gggcggagtc
     1441 ggccatcgcc gagggccgct gggcggcggc gtggggcccg gccgggatcg cctaccacgt
     1501 cgccaccagg ccgttcctgg ccggactgga ggcgccctgg atcgaccagt ggcggcgccg
     1561 gctggaggag gtccggctgc gcgggctgga atgctttgcc gccgccggtc tcggccttgg
     1621 cggcccggcg ctggcgcaag ccgaggaacg cgccaggatg ctgaccgcgc tcgcgcccta
     1681 ccgcgagacc gcgcaccgca tcctcatgga agcgctcgcg cggcgcggca acgtcgccga
     1741 ggcgctccgc gcctacgagc ggctccgggt cctgctccga gaggagctgg ggatcgcgcc
     1801 cagccccgcc gtgcaggccg tccaccggcg cctgctgctc cagagcgccg acagctagag
     1861 ccggtcaaag ctgctcggcc tgcgcgagga tgtcgtcgcg ggtcacgttg ccgaccagct
     1921 ctggatgcaa cacgcccatg tggccctgcg cgttgtgcca cagctcgtcg tcgtcgtcgc
     1981 cctggatgac atagccgcac atgcagacga tcctgagcgc catcgccaac ctccgagtcg
     2041 actcgccgcg atcttcgcgc cagctcgtcg aggccgcaag cgtgacacga ccgtgacagg
     2101 accctctggc cacccgctca ggaagcggga gacgccgccc cggcgcccgc cggcgcgtcg
     2161 gccgtctcgg ccgggaggc
//
LOCUS       DASUBI010000654         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_444411, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000654 DASUBI010000000
VERSION     DASUBI010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444411"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFU72_12660"
     CDS             <1..281
                     /locus_tag="VFU72_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5424389.1"
                     /translation="IYVPPGVVGSTPPGRARPGATPLSRREREVAALVARGLTNREIA
                     ATLCITAGTATLHVKHILTKLNLTRRTQVAIWALHAGLTVDDPRPLPR"
     gene            380..865
                     /locus_tag="VFU72_12665"
     CDS             380..865
                     /locus_tag="VFU72_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424390.1"
                     /translation="MRQITYALQFQGRTLVTSPGDRTVRITAFAPSCTVTTLLGPDGV
                     ASAVEPVPGARATCEMTVVSAATSRPRGAATVTFGPAGHRLHLRLRGHSALAGGTAAV
                     TCCALVWHVVRGEGQFADAAGLIASHLVIDARGAVTDTQFGVITLLAAPGGAEVDGVW
                     P"
     gene            1632..2486
                     /locus_tag="VFU72_12670"
     CDS             1632..2486
                     /locus_tag="VFU72_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424391.1"
                     /translation="MAAPYTGESTDPHVSGLVGSNASTGIGVLGQSAQGAGVNGISQG
                     GGDGTGVWGDCQNGRGVVGVSNAGAGVWGEAAQARGVVGVSRGDGAGVWGDCQNGRGV
                     VGVSNAGTGVWGESASGVGVVGKGAHLAGRFEGDVEVTGDIRFTALQGDCAEEFDLAG
                     PVRPEPGTVVVLGDDGAIAPSQQGYDRRVAGVVSGAGAYRPALVLDTRPSRGERLPIA
                     LLGKVYCLADAQYGPIGVGDLLTTSPTAGHAMKATDPLRAFGAVLGKALGPLVAGQEL
                     IPILVALQ"
BASE COUNT          404 a          861 c          992 g          396 t
ORIGIN      
        1 cgatctatgt cccgccaggt gtcgtcggct cgactccgcc tggccgtgct cggccagggg
       61 cgaccccgct cagtcggcgc gagcgcgagg tggcggcgct cgtcgcgcga gggctgacca
      121 accgcgagat cgccgccacg ctgtgcatca ccgcaggcac cgccacgctc catgtgaagc
      181 acatcctcac gaagctcaat ctcaccaggc gcacccaggt cgccatctgg gccctgcacg
      241 cgggcctcac cgtcgacgat ccccgcccac tgccgcgctg aatcgcgaca cgcaaatatc
      301 ccttttggga gatgtgcatg ccgcgcgccc ggcgtatgct ggcagcgatg gcacccccac
      361 cccaggcggc ggaggcacga tgcgacagat cacctatgcg ctgcaattcc agggccgcac
      421 gctggtaacg agcccggggg accgcaccgt ccgcatcacg gcctttgccc cgagctgcac
      481 tgtgaccacg ctgctcggcc cggacggcgt ggccagcgcg gtcgagcccg tcccgggtgc
      541 gcgcgcgacc tgcgagatga cggtggtgtc cgctgctacg agccgaccac ggggcgccgc
      601 gaccgtgacg ttcgggccag cggggcaccg gctgcacctc cggctacggg gccacagcgc
      661 tctcgctggc ggcaccgcgg cggtgacgtg ctgtgcgctc gtctggcacg tggtgcgcgg
      721 cgaaggccag ttcgccgatg ccgccgggct gatcgcgtca cacctcgtca tcgatgccag
      781 gggcgcggtc accgacaccc agttcggcgt catcacgctg ctagcagccc ctggtggggc
      841 ggaggtggac ggcgtgtggc cgtgaggcag gacggtgccc ggcagggacg cggtcgctga
      901 aggagccggg cagctgctgg aggcgtggac cacctgagac tggctgggct gctccagcaa
      961 cgtggcggct gtcggcctcc cgcactctct gttttcacgg cgacgcggcc gtgtccgccg
     1021 ctgtctcggg actcgtcgcc gtgaagccgc ctgggacgac gttcctggca tagcccggta
     1081 gccgctcgag actggctacg ccgcaatgct tgcgagatcc ctggggcggt ctgagagtcg
     1141 ccactgcgcg gcgcgcggct ctgacgtcgg tgctcgtcac ggttcgatct gctccagacg
     1201 ccggatttgg cggctgcaca gtggagtggc tgtcgagtgc cgtgcgccgc gcagtggcca
     1261 gcagtgattt catcacgcct gactgcatgg acgggcagaa gcggcgcatg ccgggaccgg
     1321 tacgccggag gacgcactgc cccggctcgg caaggggagt gcgatcatga gcgtcgtcac
     1381 cgctgcgcct gcactgcatc gagggccgcc gcccacatct gcgctgtcca ttcgtccggc
     1441 gcttccgtcg caggacgagg gcgggctgac cggatcaagg caactgccaa cccaagggcc
     1501 agcagctgag ggctgagata agaagtggaa cagagagccg catcgacgcg agcggatatg
     1561 gctgcccggg aaatatcact gtgcacttat catgtcggcg tgacatacca cagacacagg
     1621 aggggatcac gatggcggca ccgtacacag gcgaaagcac cgatccgcat gtctccggtt
     1681 tggtggggtc gaacgcgtcg accggcatcg gagtcctggg gcagagcgct cagggcgcgg
     1741 gcgtaaacgg gatttcccag ggcgggggcg acggcactgg cgtctgggga gactgccaga
     1801 acggccgtgg cgttgtgggg gtctccaacg ccggcgccgg ggtgtggggc gaggcggcgc
     1861 aggcgcgggg cgtggtcggc gtctcccgcg gcgatggtgc cggcgtctgg ggggactgcc
     1921 agaacggccg tggcgtcgtg ggggtctcta acgccggcac tggcgtgtgg ggggagagcg
     1981 cgagtggcgt cggtgtggtc ggcaagggcg cgcacctcgc cggacgcttc gagggggacg
     2041 tggaggtcac tggcgacatt cgcttcacgg cgctgcaggg cgattgcgcc gaagagttcg
     2101 acctcgccgg gccggtacgg ccggaaccgg ggacggtggt cgtgctcggg gatgacgggg
     2161 cgatcgcgcc gagccagcag gggtacgata ggcgggtcgc gggcgtggtc tccggcgcgg
     2221 gggcgtaccg gccggcgctg gtgctcgaca cccggccgtc gcgcggcgag cgcctcccga
     2281 tcgcgctcct ggggaaggtt tactgcctgg ccgacgcgca gtatggcccg atcggggtgg
     2341 gggatctcct gacaacctcc ccgacggcgg gccacgccat gaaggccacc gatccactgc
     2401 gagccttcgg cgctgtccta ggcaaggcgc tgggaccgct ggtcgcaggg caggaactga
     2461 ttcccatcct ggttgcgctg cagtaggccg gggagaggag gaccgacatg gccagcgaaa
     2521 agtcggggag cgcggagggc ggcgggagta gcagcggggg tgctttctcc acctcaggcc
     2581 tcgctgccaa cttgaccgat atggacaccg cgatcaaggc gactcaggac atgatcgacc
     2641 gggtcgacaa att
//
LOCUS       DASUBI010000655         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_372745, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000655 DASUBI010000000
VERSION     DASUBI010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372745"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..754)
                     /gene="rph"
                     /locus_tag="VFU72_12675"
     CDS             complement(20..754)
                     /gene="rph"
                     /locus_tag="VFU72_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5424392.1"
                     /translation="MASTYNRKRPDGRANDALRPLEIIPGFAPYADGSALIKCGDTHV
                     LCTATIEDKVPPFLTGKGRGWVTAEYGMLPRSSPQRIPRERSLESGRTKEIQRMIGRS
                     LRAVVDLRSLGERTILIDCDVIQADGGTRTASITGGFVALHDALSWLRERGLIVTLPI
                     TDFIAAVSVGVYRGTPVLDLDYAEDSQCDTDMNLVMTAGGRYIEVQGTAEGAPYSRAE
                     MSAMLDLGEKGIRQLAAAQKNALGLA"
     gene            complement(754..2100)
                     /locus_tag="VFU72_12680"
     CDS             complement(754..2100)
                     /locus_tag="VFU72_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5424393.1"
                     /translation="MDLFEAHRRELLRRRAPLADRMRPRTLDELVGQERLIGPGTLLR
                     QAIEQDRLSSLILWGPPGSGKTTLARVVAGITRAHFVQLSAVMAGVADLRREIAEARD
                     RLGMHGERTILFIDEIHRFNKAQQDAILPQVEDGTVTLIGATTENPSFEVNSPLLSRS
                     RVLVLKALDDDAIGQLLDRALVDTERGLGSEKLTLAPEARAALVNLANGDARFALNTL
                     ELAAVGAVDGVIGVEQIAAAADVRSGSYDRAGEAHFDTISALHKSIRGSDPDAALYWL
                     ARMLERGDDPLYVARRLVRAASEDVGLADPQALAVTLAAQQAVHFLGMPEGQLALAEA
                     AVYLATAPKSNTLYQAYGAAVRDVQSTRNEPVPLHLRNAPTRLMRELGYGRDYRYAHD
                     YDEALVEQQHLPDNLVGRVYYQPSSRGYEREIGERLRRWRELLARRRAESQRTKEE"
     gene            complement(2121..2297)
                     /locus_tag="VFU72_12685"
     CDS             complement(2121..2297)
                     /locus_tag="VFU72_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424394.1"
                     /translation="MVRGEEPSLYELLLELDRLEELREDLQERGLRTLEEVERRIAEL
                     HQRIDELEDAEPAP"
     gene            complement(2309..>2754)
                     /locus_tag="VFU72_12690"
     CDS             complement(2309..>2754)
                     /locus_tag="VFU72_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424395.1"
                     /translation="SQRALRAGDDETRQRARNAYTLLLHIIAFVGLSMIYINKLRSLF
                     SATAVLVLVFLLLLQLTEGVQAAAARRLVYALAGGVVLGEITWVLNYWRATGWTGGAA
                     LLIFFYLIAGLILNQLRGGVSSRDLAEYGGVSAVAFAIVAVAVLR"
BASE COUNT          419 a          994 c          916 g          425 t
ORIGIN      
        1 ctggcaatga ccagtttctt cacgccagtc ccagcgcgtt tttctgggcc gccgcgagct
       61 ggcgaatgcc tttttcgcca agatcgagca tggcgctcat ctccgcgcgc gagtagggcg
      121 ctccctccgc ggtgccctga acctcgatgt agcggccgcc ggcggtcatc acaagattca
      181 tgtcggtgtc gcactgcgaa tcctcggcgt agtcgagatc cagcaccggc gtgccgcgat
      241 acacgcccac cgacaccgcg gcaataaaat ccgtgatcgg cagcgtcacg atcagcccac
      301 gctcacgcag ccagctcagc gcgtcatgca gcgcgacgaa gccgccggtg atgctggcgg
      361 tgcgcgtgcc gccgtcggcc tggatcacgt cgcagtcgat cagaatggtg cgctcgccca
      421 gcgaacgcag gtcgaccacc gcccgcagcg agcgcccgat catgcgctga atctccttgg
      481 tgcgcccgct ctccagcgaa cgctcgcgcg ggatgcgctg aggcgagctg cgcggcagca
      541 tgccgtactc ggccgtgacc cagccgcgcc ccttgcccgt cagaaagggc ggcactttgt
      601 cttcgatggt ggccgtgcag aggacgtgcg tgtcgccgca cttgatcagc gccgagccgt
      661 cggcgtaggg cgcgaagccg ggaatgatct ccagcgggcg cagggcgtcg ttggcgcggc
      721 cgtccggccg cttgcggttg taggtcgagg ccattactcc tctttcgtgc gctgactctc
      781 cgcccggcgc cgcgccagca gctcgcgcca gcggcgtaac cgctcgccga tctcccgctc
      841 atagccgcgg ctgctcggtt ggtagtagac gcgacccacc aggttatccg gcaggtgctg
      901 ctgctcgacc agcgcctcgt cgtagtcgtg ggcgtagcga tagtcgcgcc cgtagcccag
      961 ctcgcgcatc agccgtgtgg gcgcgttgcg caggtgtagc ggcaccggct cgttgcgcgt
     1021 ggactgcacg tcgcgaaccg cggcgccgta ggcctggtac agcgtgttgc tcttgggcgc
     1081 ggtggccagg tagaccgccg cctcggccag cgccagttgc ccctccggca tgcccaggaa
     1141 gtgaaccgcc tgctgcgccg ccagcgtgac agccagcgcc tgcggatcgg ccaaccccac
     1201 gtcttccgac gcggcgcgca ccaaccggcg cgcgacgtag agcggatcgt caccgcgctc
     1261 gagcatgcgc gccagccagt agagcgcggc gtcggggtcc gagccgcgaa tcgacttgtg
     1321 cagcgccgag atggtgtcga agtgcgcctc tcccgcgcgg tcgtagctgc cgctccgcac
     1381 gtcggccgcg gcggcgatct gctccacccc gatcactcca tcgaccgcgc ccaccgccgc
     1441 cagctccagc gtgttcagcg cgaagcgcgc gtcgccgttg gcgaggttga ccagggcggc
     1501 ccgcgcctcg ggcgcgagcg ttagcttctc ggaccccagc ccccgctcgg tatcgaccag
     1561 ggcccggtcg agcaactggc cgatcgcatc atcatcgagc gccttcagca ccagcacccg
     1621 cgagcgcgag agcaggggcg agttgacctc gaaggaggga ttctcggtgg tggcgccgat
     1681 cagggtgacc gtgccgtcct cgacctgcgg cagaatcgcg tcctgctgcg ccttattgaa
     1741 gcggtgaatc tcgtcgatga agaggatggt gcgctcgcca tgcatcccca gccggtctcg
     1801 tgcctcggcg atctcgcggc gcaggtcggc cacaccggcc atcaccgccg agagttggac
     1861 gaagtgggct cgggtaatgc cggcgacgac ccgcgccagc gtcgtcttgc cgctgcccgg
     1921 cggcccccag aggatcagcg agctgagccg gtcctgctcg atcgcctggc gcaggagcgt
     1981 gcccgggccg atcagccgct cctggcccac cagttcgtcc agcgtgcgcg gccgcatacg
     2041 gtccgccagc ggcgcgcgac ggcgcagcag ttcccggcgg tgtgcctcga acagatccat
     2101 gcgccttccc tctctcgccg ctacggcgcc ggctccgcgt cctccagctc gtcgatgcgc
     2161 tggtgcagtt cggcgatgcg ccgctcgacc tcctccagcg tgcgcagccc gcgctcctga
     2221 aggtcctcgc gcagctcctc caggcggtcc agctcaagca gcaactcgta gagggacggc
     2281 tcctcgccgc gcaccatcgc cgccgcctct agcgcagcac cgcgacggcc acgatggcaa
     2341 aggcgacggc gctgacgccg ccgtactccg ccagatcacg cgagctgacg ccgccgcgca
     2401 actggttcag gatcaggccg gcgatcaggt agaagaagat caagagggcg gcgccgccgg
     2461 tccagccggt ggcgcgccag tagttcagca cccaggtgat ctcgcccagc actacaccgc
     2521 cggccagggc gtaaaccagc cggcgcgccg cggcagcctg caccccttcg gtcagttgca
     2581 gcagcagcag gaagaccagc accagcacgg ccgtcgccga gaagagactg cgcagcttgt
     2641 tgatatagat catcgaaagg ccgacgaagg cgatgatgtg gagcaagagc gtgtaggcgt
     2701 tgcgcgcccg ctgccgtgtc tcgtcgtcgc cggcgcgcag cgctcgctgc gaca
//
LOCUS       DASUBI010000656         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1015768, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000656 DASUBI010000000
VERSION     DASUBI010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015768"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFU72_12695"
     CDS             <1..872
                     /locus_tag="VFU72_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5424396.1"
                     /translation="RIKGTPSGLALKTDCNPRYCYLDPYLGGMHAVAEAARNVSCVGA
                     QPLAITNCLNFGNPEKPEVYYQLERAVAGMAAACRALETPVVSGNVSLYNESGAEAIY
                     PTPTVGMVGLLADVRRRVGLALQTEGVVYLVGPVEATLGASEYLSTIHGKVAGAPPAL
                     SLPLEFAVQDVVRSAIERGLVAAAHDCSEGGLAVALAESCLADGVGGRFTLQPLLAVN
                     QRLDRTLFGEAASRIVVQAQAGQEAALERALAGAAVPFLRLGAAGGERLELPGFLDIE
                     LAALQDAWSAGLR"
     gene            944..2341
                     /gene="purF"
                     /locus_tag="VFU72_12700"
     CDS             944..2341
                     /gene="purF"
                     /locus_tag="VFU72_12700"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5424397.1"
                     /translation="MPFDEPREACGVFGIYAPGEDVARLTYFGLYALQHRGQESAGIA
                     TADGHIMRIHTRMGLVHTAFDEPTLAGLTGHLAIGHTRYSTTGASRENNAGPFLVEQG
                     QIAVAHNGNLVNSEELRQELLGRGIPLEGTTDSEALAWMIALDDAPTIADRVRRSMER
                     FVGAYSLAILTPEALVAVRDPLGIRPLCLGRLGEGWVVASETCALATIGAQFERTVEP
                     GEIVVIDAEGFHSYPAPERPEHQALCLFEFIYFARPDSTLMGKRLHLARQRMGAELWH
                     EDPIEADIVVPLPDSAVPAAIGYARASGIPYAEGLIKNRYIGRTFIQPDQRLREQGVK
                     LKFNALPEVLEGKRVVLVDDTIVRGTTSKPIVDLLRQNGAREVHMRVHAPPIKWPCYF
                     GVDMARREELIAANLSIEEVGRTIGADSIHYLSLDGLMRAIGLPRANFCSACLTGEYP
                     LPVDGAVQPIALVRR"
     gene            2468..>2752
                     /locus_tag="VFU72_12705"
     CDS             2468..>2752
                     /locus_tag="VFU72_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424398.1"
                     /translation="MDSLCGACPQPHTPYDCGQFLPRCLAGDLAAWLRVWAWPFTCPQ
                     HRGFDGRRLGRQARPLVGNVQAAIELLVHGDLGAGVAEPVGTRLQLQPVRP"
BASE COUNT          397 a          927 c          994 g          434 t
ORIGIN      
        1 tgcgcatcaa ggggacgccg agcgggctgg cgctcaagac cgactgcaac ccgcgctact
       61 gctacctcga cccctacctg ggcgggatgc acgcggtggc cgaggccgcg cgcaacgtga
      121 gctgcgtcgg cgcccagccg ctggcgatca ccaactgcct caacttcggc aatcccgaga
      181 agcccgaggt gtactaccag ctcgagcgcg ccgtagccgg catggcggcg gcctgccgcg
      241 cgctggagac ccctgtcgtc agcggcaacg tcagcctcta caacgagtcg ggcgccgagg
      301 cgatctaccc cacgccgacc gtcggtatgg tcggcctgct ggcggatgtc aggcgtcgcg
      361 tcgggctggc cctgcagacg gagggcgtgg tctatctggt tggcccggtc gaggccacgc
      421 tcggcgccag cgaatacctg agcacgatcc atgggaaagt ggccggggcg ccgccggcgc
      481 tctcgctgcc gctggagttc gcggtgcaag acgtggtgcg ctcggccatc gagcgggggt
      541 tggtggcggc ggcgcacgat tgctccgagg gagggctggc ggtggcgctg gccgagtcct
      601 gcctggccga tggggtcggg ggccgcttca cgctccagcc gctccttgcg gtgaaccagc
      661 gcctggaccg gacgctcttc ggcgaggcag catcgcggat cgtggtgcag gcgcaggcag
      721 gacaggaagc ggcgttggag cgcgcgctgg ccggggcggc tgtaccgttc ttgcggttgg
      781 gtgctgccgg cggcgagcgg ctggagctgc cgggattcct cgacatcgag ctggccgcat
      841 tgcaggacgc ctggagcgct ggattgcgct aatgtacgta cgaaagctgt ggtagactga
      901 aaaggttcgg cggggtagcc gggacggaga ggggcgcgtg gtcatgccgt tcgatgagcc
      961 acgtgaggcg tgcggggtct ttggcatcta tgccccgggc gaggacgtcg ccaggctgac
     1021 ctacttcggc ctctacgcgc tgcagcatcg cggccaggag agcgccggaa tcgccaccgc
     1081 cgacgggcac atcatgcgca tccacacgcg catggggctg gtccacaccg ccttcgacga
     1141 gcccacgctg gccgggctga ccggccatct tgccatcgga cacacccgct actccacgac
     1201 cggcgccagc cgcgagaaca acgccggccc gttcctggtc gagcagggcc agatcgcggt
     1261 ggcgcacaac ggcaacctgg tgaacagcga ggagctgcgc caggagctgc tggggcgggg
     1321 gatcccgctg gagggcacga ccgactccga ggcgctggcc tggatgatcg cgctcgatga
     1381 cgcgccgacc atcgccgacc gggtgcgccg ctcgatggag cgcttcgtgg gcgcctacag
     1441 cctggcgatc ctgacgcccg aggcgctggt ggcggtgcgc gatccgctcg ggatccgccc
     1501 gctctgcctc ggccggctcg gggagggctg ggtggtggcc tcggagacct gcgccctggc
     1561 cacgatcggc gcgcagttcg agcgcacggt cgagccgggt gagatcgtgg tgatcgacgc
     1621 cgagggcttc cactcttacc cggcgccgga acggcccgag catcaggcgc tttgcctctt
     1681 cgagttcatc tacttcgccc gtccggacag cacgctgatg ggcaagcgcc tgcatctggc
     1741 gcggcagcgc atgggcgccg agctctggca cgaggacccg atcgaggccg atatcgtcgt
     1801 gccgctgccc gattccgccg tgccggcggc gatcggctac gcgcgggcct ccggcatccc
     1861 ctatgccgag ggcctgatca agaatcgcta tatcggccgc acctttatcc agcccgacca
     1921 gcggctgcgc gaacagggcg tcaagctcaa gttcaacgcg ctgccggagg tgctggaggg
     1981 caagcgggtc gtgctggtgg acgacaccat cgtgcgcggc accaccagca agccgatcgt
     2041 cgacctcttg cggcagaacg gcgcccgcga ggtgcatatg cgggtgcacg cgccgccgat
     2101 caagtggccc tgctacttcg gcgtcgatat ggcgcggcgc gaggaactca tcgccgctaa
     2161 tctctcgatc gaggaggtcg gccggaccat tggcgccgac tcgatccatt accttagttt
     2221 ggacgggctg atgcgcgcca tcgggctccc gcgcgccaac ttctgcagcg cctgcctgac
     2281 cggagagtac ccactgccgg tggatggcgc cgtgcagccg atcgccctcg tacgccgcta
     2341 ggccaactgt cggtgttcag tgactctgtc atggtaactg gtcagtgact ctgtcacccc
     2401 ctccgttgca tcaacgacgc cagggatggt cgggcgccgg cttgcgcggg ggctggggcg
     2461 tggagttgtg gacagcctct gcggagcctg cccacaaccc cacaccccct acgactgcgg
     2521 ccagttcctc ccgcggtgtc tcgcgggcga tctcgccgcc tggctgcggg tctgggcgtg
     2581 gccctttacg tgcccgcagc accggggctt cgacgggcgc cgcctgggtc gccaggcgcg
     2641 tcccctggta ggcaacgtcc aggcggccat cgagctgctc gtgcacggtg acctcggcgc
     2701 gggcgtagct gagccggtcg ggacccggct gcagttgcag ccggtgcggc cc
//
LOCUS       DASUBI010000657         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_481754, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000657 DASUBI010000000
VERSION     DASUBI010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481754"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VFU72_12710"
     CDS             <1..1209
                     /locus_tag="VFU72_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5424399.1"
                     /translation="LLVPTAADADRIRGLIRELVTAYQAGQAGRTDGLLLPDVKATCE
                     RLADDVLGMGILEPLMRDRSVEEITVNGPARVWVYQHGAWSLVQGLRFESNDDLLALM
                     KRVVGNLGGRLDEAAPRAEVRLGDGSRLNVVIPPVAVDGCAVTIRKFLLQNHSLADLV
                     TLGTLSREAADFLSAAVCGGVNILVSGGTGAGKTTLLNALGAALGTRNERVVTIDEVV
                     ELQLRTLLPACVALQTRGGNTEGAGELTIRDLVRTSLRMRPHRIIIGEVRGEEALDML
                     LALNSGHDGSLGTIHANSPADALAKLATLARMAPEQLPADVLTEMVAATVELVVQLRT
                     DPATNRRRVVHIFEVTGLEGGRLAGQDLWLMEQGRLVWTGLRPQCLDKIQARDIPYAL
                     PHVFAALAEP"
     gene            1295..>2008
                     /locus_tag="VFU72_12715"
     CDS             1295..>2008
                     /locus_tag="VFU72_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5424400.1"
                     /translation="MLLFGFAILAATGLFSTCAALTTNSAVGAGQRPPAWPGQWERFL
                     TRAGVRSVVSPGAFVAISLGAAALSGALTLLALHWVLPAVVVGGAGAALPALYLEHRQ
                     ARRQREVQAALVDAISQLRASLASGQSVQQGLTALARTGPRVLRPECADLARDLQRYG
                     VTPALRRLQARLADPLVDTFASALIMNDRSGGRQIGRVLEQLARAARAELLMAEESRA
                     QQASTVLQARIIAVMPWALL"
BASE COUNT          285 a          673 c          734 g          316 t
ORIGIN      
        1 ctactggtcc ccacagccgc ggacgcggac cggattcgcg gtctcatccg cgaactggtc
       61 acggcgtatc aggccgggca ggcggggcgc actgacggtc tcttgctgcc cgacgtcaag
      121 gcaacctgcg agcggctcgc cgacgatgtg ctcggcatgg gcattctcga gccgctgatg
      181 cgcgaccgca gcgtcgagga gattacggtc aacggaccgg cgcgcgtctg ggtctaccag
      241 cacggcgcgt ggtcgctcgt ccagggtctc cgctttgaga gcaacgacga tctgctggcg
      301 ctgatgaagc gggtggttgg caatctgggc ggccgactcg acgaggcggc gccgcgtgcc
      361 gaggtgcgcc tcggcgacgg ctcgcggctc aacgtcgtca tcccgcccgt ggcggttgac
      421 ggctgtgctg ttaccatccg caagttcctg ttgcaaaacc actcgcttgc ggatctcgtc
      481 acgctcggca cgctctcgcg cgaggccgct gacttcctga gcgcggccgt gtgcggcggt
      541 gtcaatatcc tcgtcagcgg cggcaccggc gccggcaaga cgaccctcct caatgcgctc
      601 ggcgcggcgc tcggaacgcg caacgagcgt gtggtcacga ttgatgaagt ggtcgagctg
      661 caactccgca ccctgctgcc ggcctgtgtg gcgctccaga cgcggggcgg gaacaccgaa
      721 ggagccggcg agctgaccat tcgcgacctc gtgcggacgt cgctgcggat gcggccgcac
      781 cggatcatca tcggcgaagt acgcggcgag gaggcgctgg atatgctgct cgccctcaac
      841 agcggtcatg acggtagcct cggcacgatc cacgccaact ctcccgccga tgcgctcgcc
      901 aaactcgcga ccctggcgcg catggcgccc gagcagttgc ccgccgatgt cctgaccgag
      961 atggtcgcgg cgacggtcga actggtcgtg cagttgcgca ccgatcccgc cacgaaccgg
     1021 cggcgggtgg tgcatatctt cgaggtgacc ggcctggagg gtggccgcct ggctggccag
     1081 gatctctggc tcatggagca ggggcggctt gtctggaccg gactgcggcc ccagtgcctc
     1141 gacaagatcc aggcgcgtga catcccctat gccctgccgc acgtcttcgc cgcgctggcc
     1201 gagccctgag tacggccagg agtgtcgcgg tgagagcgac gtgagagcgt ggtgagagcg
     1261 aaggcgcgta gcgtaccagc cagggaggtg ggcgatgctg ctgttcgggt tcgcgatcct
     1321 ggcggccacc gggctgttca gcacctgcgc cgcgctgacc acgaatagcg cggttggggc
     1381 ggggcagcgc ccgccggcct ggcccggaca atgggaacgc tttctgacgc gggcaggcgt
     1441 gcggagtgtg gtctcgccag gggcgttcgt cgccatttcg ctcggcgccg cagcactgag
     1501 tggcgcgctg acgctgctgg cgctgcactg ggtgctcccc gcggtcgtgg taggcggcgc
     1561 cggtgcggcg ctcccggccc tgtatttgga gcaccggcag gcgcggcggc agcgcgaagt
     1621 gcaggcggcg ctggtcgacg cgatcagcca gctgcgcgcg agcctggcga gcgggcagtc
     1681 ggtgcagcag ggcctcaccg cactggcgcg gaccggcccg cgtgtgttgc ggccggagtg
     1741 cgccgacctg gcgcgtgacc tgcaacgcta cggggtgacg cccgctctgc gtcggctgca
     1801 agcgcgcctc gccgacccgc tggtggacac cttcgcgagc gcgctcatca tgaatgaccg
     1861 gagcggcggc cgccagatcg gccgggtgct cgaacaactg gcccgcgccg cccgcgccga
     1921 gctcctgatg gcggaagaat cccgggccca gcaagcgagc accgtgttgc aggcgcgcat
     1981 tatcgcggtc atgccctggg cgctgctg
//
LOCUS       DASUBI010000658         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_58990, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000658 DASUBI010000000
VERSION     DASUBI010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58990"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1787)
                     /locus_tag="VFU72_12720"
     CDS             complement(132..1787)
                     /locus_tag="VFU72_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5424401.1"
                     /translation="MARDFSPIELRVLWNRLIAIVDEAAATLVRTSFSTLVRESNDFA
                     CVLFDAQGRSLAQNSAAIPSFIGTLPITMRHFLARFPADSLRPGDVLITNDPWLATGH
                     LPDITIARPIFRNGQLVAFSGSVAHSPDIGGRIRSPDARQVYEEGLRIPPMKLYAAGQ
                     RNELLLELLEANVRVPRQVTGDLLAQVAANELLARRLLVLLDEQGLDDLGELARVIQE
                     RSARAMRRAIAALPDGSYEGEAHPDGLDEPLDIRLRLTIAGEEITVDYAGSSPQVDYA
                     VNTVLNYTFAYTAYPLKCITSPAIPNNEGSFEPIRVSAPEGSLLNPRFPAPVGGRALI
                     GHFLAAAIFQALAPVAPAAVQAASGSPLWCLHLTGEERGRAFSSINFLNGGQGASLRQ
                     DGLSCLSFPSNVANTPVEVMEALAPLVVERKALRPDSGGAGRQRGGLGQELQVRITAE
                     QPVTVAFLADRLRRPADGLLGGGPGASGQVLLNGAPINPKRQIVVKGGDTLLLATPGG
                     GGFGEPPERDQQALAQDLANGLVAPEAAAGVYGAGRVAPAEPE"
     gene            complement(1777..>2173)
                     /locus_tag="VFU72_12725"
     CDS             complement(1777..>2173)
                     /locus_tag="VFU72_12725"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU5424402.1"
                     /translation="LLRFETVYAGRYERAIPGVPVEAVTWRLSASGPPPALPDEPSRE
                     ASVPPLAEATKGERPAYFPALGGFAPTPVYDRERLRPGMRGQGPAVIEEREAPVVVGP
                     RDHFQIDGRGNVVIAIEQASGERGEAGGA"
BASE COUNT          290 a          791 c          797 g          295 t
ORIGIN      
        1 gtgctgacgt cggccaggaa gatcacgccg cagagcgcca ggatctcgcg gctcaggctc
       61 tcgcccagcc caccccgcag ccggccggtc cggcgcgccg ccgtgtcgac gtcaggcacg
      121 cgcgccggcc ctcactcggg ctcggccggg gcgacacgtc ccgcgccata gacgccggca
      181 gccgcctcgg gcgcgacgag cccgttggcc agatcctgcg ccagggcctg ttggtcgcgc
      241 tcgggcggct cgccgaagcc gccgccgccg ggtgtcgcca gcagcagggt gtcgccgccc
      301 ttgacgacga tctggcgctt agggttgatc ggcgcgccgt tgagcagcac ctggccggac
      361 gcgccgggac cgcccccgag caggccgtcg gccggccggc gcagccgatc ggccaggaag
      421 gccaccgtca ccggctgctc ggccgtgatg cgcacctgga gctcctgtcc taacccgccg
      481 cgctgccgcc cggcgccgcc ggagtccggc cgcagcgcct tgcgctcgac caccagcggc
      541 gccagcgcct ccatcacctc gaccggcgtg ttggcgacgt tgctggggaa gctgagacag
      601 gagaggccgt cctgccgcag ggaggcgccc tggccgccgt tgaggaagtt gatgctgctg
      661 aaggcgcggc cgcgctcctc gccggtcagg tgcaggcacc agaggggcga gccgctggcc
      721 gcctgaaccg ccgccggcgc gaccggcgcc agcgcctgga agatggcggc cgccaggaag
      781 tgcccgatca acgcgcgccc gccgaccggc gccgggaagc gcgggttgag gagactcccc
      841 tcgggcgcgc tgacccggat cggctcgaac gagccctcgt tgttgggaat ggccgggctg
      901 gtgatgcact tgagcgggta ggcggtgtag gcgaaggtgt agttgagcac ggtgttgacc
      961 gcgtagtcca cctggggcga cgagccggcg tagtcgaccg tgatctcctc gcccgcgatc
     1021 gtcagccgca gccggatgtc gagcggctcg tcgagcccgt ccgggtgggc ctcgccctcg
     1081 tagctgccgt cgggcagggc ggcgatggcg cgccgcatgg cgcgcgcgga gcgctcctgg
     1141 atcactcgcg ccagctcgcc caggtcgtcc agtccctgtt cgtccagcag caccagcagc
     1201 cggcgcgcca gcagctcgtt ggcggccacc tgggccagca gatcgccggt gacctgccgg
     1261 ggcacgcgca cgttggcctc cagcagctcc agcagcagct cgttgcgctg tccggccgcg
     1321 tagagcttca tgggcgggat gcgtaagccc tcctcgtaga cctggcgcgc gtcggggctg
     1381 cggatgcgcc cgccgatgtc cggcgagtgg gccacgctcc ccgagaaggc caccagctgt
     1441 ccattccgga agatcgggcg agcgatggtg atatcgggca gatgaccggt cgccagccag
     1501 gggtcgttgg tgatcagcac gtcgccgggc cgcagcgagt cggccgggaa gcgcgccagg
     1561 aagtgacgca tggtgatcgg cagcgtgccg atgaagctcg ggatggccgc gctgttctgc
     1621 gccagcgagc gaccctgggc gtcgaacagg acgcaggcga agtcgttgct ctcgcgcacc
     1681 agcgtcgaga aggaggtgcg caccagcgtg gcggccgcct cgtcgacgat ggcgatcagg
     1741 cggttccaga ggactcgcag ctcgattggg ctgaagtcac gcgccaccgg cttcgcctcg
     1801 ctctccgctc gcttgctcga tcgcgatcac cacgttgccg cgcccgtcga tctggaagtg
     1861 gtcgcgtggt cccacgacca cgggcgcctc ccgctcctcg atcaccgccg gcccctggcc
     1921 gcgcatcccc ggccgcagcc gctcccggtc gtagaccggc gtcggcgcga agccgcccag
     1981 cgccgggaag taggccggcc gctcgccctt cgtcgcctcg gccagcggcg gcacgcttgc
     2041 ttcgcgggat ggctcatccg gcagcgcggg aggcgggccg ctggcgctga gtcgccaggt
     2101 gacggcctcg accggcaccc cggggatggc gcgctcataa cgccccgcgt agaccgtctc
     2161 gaagcgcagc agc
//
LOCUS       DASUBI010000659         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_993061, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000659 DASUBI010000000
VERSION     DASUBI010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993061"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFU72_12730"
     CDS             <1..485
                     /locus_tag="VFU72_12730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424403.1"
                     /translation="LVPVVEQACQWAQVAAQNKGVELRSAVAEADVRVLGNADALLSV
                     VGNLLSNAVRYTPPGGRVSVRLGGDATTVWLEVEDTGIGIAPEARERIFNKFWRAPDA
                     RDLDVRGLGLGLALVKQMTEAHDGRVTVVSTPGQGSTFRVELPRYRATGEGGEHGGGT
                     "
     gene            469..966
                     /locus_tag="VFU72_12735"
     CDS             469..966
                     /locus_tag="VFU72_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5424404.1"
                     /translation="MAEEHEGGALAAQVAGQADLIAQQRMTISELETPVLQVWDGILA
                     LPLVGSLDTARTQEMNEALLQRIVETGSEIVILDITGVPVVDTAVARHLLETVAAARL
                     LGAEVLIVGLSPRTALTLVHLGLDLAGVTTRTTMAKGLQLAFARLGLAVVPRRDTGRA
                     AAGEE"
     gene            973..1362
                     /locus_tag="VFU72_12740"
     CDS             973..1362
                     /locus_tag="VFU72_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5424405.1"
                     /translation="MAPVPIIHIGETLIATVQEDLRDQDALGLQQHLSERLVHTGARG
                     VLLDLSVAETVDSFLGRLLGDIAAGARLLGAHTVVVGIQPAVAITLVELGLELRGVYT
                     ALTVEKGLARLRALLVQEERLHGHRGQ"
     gene            1343..1765
                     /locus_tag="VFU72_12745"
     CDS             1343..1765
                     /locus_tag="VFU72_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEU5424406.1"
                     /translation="MATGVNSPIRVAIAGEEGIIAARQAARELAKALGFRAIDQSRIT
                     TAVSELARNVVRYATERSGEVMLRPLGAHEGGAGIEIVVRDNGPGIADVGLALQEGYT
                     SGRGLGMGLPGTRRLMDEMAIDSGPGRGTTVTIRKRLR"
     gene            1767..2174
                     /locus_tag="VFU72_12750"
     CDS             1767..2174
                     /locus_tag="VFU72_12750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424407.1"
                     /translation="MDPEAVEIPVASRAGVEEVRRAARAMASALGFAQEEAEMVVLAA
                     IELATNLHRYAREGRIRFTPLAGAPGVGIEVTSRDAGPGIPDRGAALRDGFSTGGGRG
                     NGLPAVRRLMDEFSVVSGTDGTTIVARKWRSTP"
     gene            2156..2785
                     /locus_tag="VFU72_12755"
     CDS             2156..2785
                     /locus_tag="VFU72_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5424408.1"
                     /translation="MAFDPIAIAAATRPHPHEMVSGDLWSVQWAAGACRIAVIDGLGH
                     GSPAHAAALAAVAALAAAPDLPPDGALARCQRPLTGTRGAAIGIVRIDLAAGRLTFAG
                     VGNVEGRLWQPGGEQRLLAQRGIVGGTMPTIRPVDYPLGVDWMLILHSDGISDRFTSA
                     ALPGWGGPIQALADAILQHHGRLADDATVVLARPCRAAPTGNPGAERTG"
BASE COUNT          427 a         1015 c         1088 g          418 t
ORIGIN      
        1 ccctggtgcc ggtcgtcgag caggcctgcc agtgggccca agtcgctgca cagaacaagg
       61 gggtcgagct gcgcagcgcg gtggccgagg ccgacgtgcg cgtgctgggc aacgccgatg
      121 cgctgctgag cgtcgtcggg aatctgctct cgaatgcggt ccgctacacc ccgcccgggg
      181 gccgggtgag cgtgcgcctc ggcggcgacg cgacgaccgt ctggctcgag gtcgaggaca
      241 ccggcatcgg gatcgccccg gaggcgcgcg agcgcatctt caacaaattc tggcgtgccc
      301 cggacgcgcg cgaccttgat gtgcgcggac ttgggctcgg gctcgcgctc gtcaagcaga
      361 tgaccgaggc tcacgacggc cgggtgaccg tcgtgagcac gcccggccag ggcagcacct
      421 tccgcgtgga gctgccgcgt taccgcgcga cgggagaagg aggggagcat ggcggaggaa
      481 catgagggcg gggcgttggc cgcgcaagtc gctggtcagg ccgatctgat cgcccagcag
      541 cgcatgacga tcagcgagtt ggagacgccg gtcctccagg tgtgggacgg tatcctcgcc
      601 ctgcccctcg tcggctcgct cgacacggca cggacgcagg agatgaacga ggcgctgctc
      661 cagcggatcg tcgagaccgg ctcggagatc gtcatcctgg acatcaccgg cgtgccggtc
      721 gtggacaccg cggtggcccg ccacctgctc gaaacggtcg ccgccgcgcg gctgctcggc
      781 gcggaggtac tgatcgtcgg cctctcgccc cgcaccgcgc tcaccctggt ccacctcggc
      841 ctcgatctcg ccggggtcac cactcgcaca accatggcga aagggctgca gctcgccttc
      901 gcccgcctcg ggctggcggt cgtgccccgc cgcgacacgg ggcgcgccgc cgctggagag
      961 gagtgagacg cgatggcccc tgtgccgatc atccatattg gcgagacgct gattgctacc
     1021 gtccaggagg acctgcgcga ccaggatgcg ctcggcctgc agcagcacct gagtgagcgg
     1081 ctggtgcaca ccggcgcgcg cggcgtgctc ctcgaccttt cggtcgccga gacggttgac
     1141 tcgttcctcg ggcgtctgct gggcgacatc gccgccggcg cgcgactcct cggtgcgcac
     1201 accgtcgttg tcggcatcca gccggcggtg gcgatcaccc tcgtcgagtt ggggctggaa
     1261 ctgcggggtg tctacaccgc gctgacggtg gagaagggcc tcgctcgcct ccgcgcactg
     1321 ctggtgcagg aagagcggct gcatggccac cggggtcaat agtcccatac gcgtcgccat
     1381 cgccggcgaa gaagggatca tcgccgcgcg ccaggccgcc cgcgaattgg cgaaggcgct
     1441 cggcttccgc gcgatcgacc agagccgcat caccaccgcc gtctccgagc tcgcgcgcaa
     1501 cgtggtgcgc tacgcgacag agcgcagcgg cgaggtgatg ctgcgcccgc tcggcgcgca
     1561 cgagggcggc gccggaatcg agatcgtcgt ccgcgacaac gggccgggga tcgccgatgt
     1621 gggcctggcc ctgcaggagg gctacacctc ggggcgcggc ctcgggatgg ggctgcccgg
     1681 cacccggcgg ctcatggacg agatggcgat cgacagcggg ccgggacgtg gcacgaccgt
     1741 gacgatccgc aagcggctcc gctagcatgg acccggaggc ggtagagatc ccggttgcga
     1801 gccgcgcggg cgtcgaagag gtgcggcggg ccgcgcgggc gatggcgtcg gcactcggct
     1861 ttgcccagga agaggcggag atggttgtcc tggccgccat cgagttggcc acgaacctgc
     1921 atcgctacgc tcgggagggg cgcatccgct tcaccccgct cgccggggcg cccggagtgg
     1981 gcatcgaggt gaccagccgc gacgccgggc cgggcatccc tgaccgcggc gcggcgctgc
     2041 gcgacggctt cagcaccggc ggcgggcgtg gcaatggtct cccggcggtt cgccggctga
     2101 tggatgagtt cagcgtcgtg tccggcaccg acggcacgac gattgtagcc cgcaaatggc
     2161 gttcgacccc atagccatcg ccgcggcgac gcggccccac ccgcacgaga tggtctccgg
     2221 cgacctctgg tcggtccagt gggccgccgg ggcctgtcgc atcgcggtca tcgatgggct
     2281 tggccacggc tcacccgccc acgcggcggc cctcgccgcc gtggcggccc tcgccgccgc
     2341 accggacctc ccgcccgacg gggcgctggc gcgctgccag cgcccgctga ccggcacgcg
     2401 cggcgcggcg atcgggatcg ttcggattga tctggccgca gggcggctca ccttcgcggg
     2461 ggtcggcaac gtcgagggcc ggctctggca gccgggcggc gagcagcgac tgctcgccca
     2521 gcgcggcatt gtcggaggga cgatgccgac catccgcccg gttgactacc cgcttggggt
     2581 tgactggatg ctcatcctgc acagcgatgg gatcagcgac cgcttcacga gcgcggcgct
     2641 tccgggctgg ggtggaccta tccaggcgct ggccgacgcg attctccagc accacggtcg
     2701 tctggccgac gacgcgaccg tggtgctcgc caggccatgc cgcgccgcgc cgaccggcaa
     2761 tccaggtgcc gaacggaccg gatagctgca ctacggtgat caggcaggag gctgtagcca
     2821 tccatgtagc caagcgcctg gaacgcggtg gcagccagtc gcgaacggga atgatgggtg
     2881 ccccatgctg gcggggaagt ggataccagc ggcagcgtag tgacgaccct gccgccagtt
     2941 tgtaaata
//
LOCUS       DASUBI010000660         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1285947, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000660 DASUBI010000000
VERSION     DASUBI010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285947"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFU72_12760"
     CDS             complement(<1..512)
                     /locus_tag="VFU72_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424409.1"
                     /translation="MLVMASLASAVTAYAGPSRPAAPTSATVLTAAPSTGASDITDLG
                     ANGWSVQSSAVATQTGAQISTPGFNTSTWLPVANDDAGAPGTEVEALAQNGMCPGDTA
                     LQPVNQSDSGPNSVFFSNNIQSCYGFMSKIGADTVSEFSVPWWWRTDFTPNLAAGQTA
                     TLVVNGVIGTA"
     gene            complement(665..1870)
                     /locus_tag="VFU72_12765"
     CDS             complement(665..1870)
                     /locus_tag="VFU72_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5424410.1"
                     /translation="MDKSSPPASTPVATTTAVIRVINERAVYEQIRRLGTASASQLVA
                     ATGLSKATIGLAFGSLERAGLISQVGHRVGQVGRAPRLYEIRPDAGRAMAVDVGASWI
                     RLAVADLSGAITARADHRVRALAASALAKQIGELARATAADAGVTLADITHTVLGFPG
                     IYQPDADRIMAAPNLPGWEEPGSVVALRDQLGPRFSIDNDVNLATVAEQKHGAGAGAG
                     NFVFVSFGTGIGMGIVIGGDLYRGSSGRAGEISFLPLGDGPGQLLAPNVKQHGILETV
                     ASADGVLSAAKRIGMTATSAEQVFDAARSGDHAAIHVVHDEASHLARALAAVIAVLDP
                     GLIVIGGGVGGNADLLLEPTKRLLGDLLALSPPPIEMSILGREAVVLGGLAAGLDRAR
                     DAVLAQIQA"
BASE COUNT          354 a          765 c          773 g          371 t
ORIGIN      
        1 gccgtgccga tcacgccatt gacaaccagc gtggcggttt gcccggcggc cagattcggc
       61 gtgaagtcgg tgcgccacca ccagggcacg ctgaactcgc tgaccgtgtc agcgccgatc
      121 ttgctcatga agccgtagca agactgaatg ttgttggaga agaacacgct gttcgggccg
      181 ctgtcgctct gattcacggg ctgcagggcg gtatcgccag ggcacatgcc gttctgcgcg
      241 agcgcctcga cctcggtgcc tggcgctccc gcgtcgtcgt tggccaccgg cagccaggta
      301 gacgtgttga agccgggcgt ggagatctgc gcgccggtct gggtggccac ggcgctgctc
      361 tgaaccgacc agccgttcgc gccgaggtcg gtaatgtcgc tcgcgccagt actcggagcg
      421 gcagtcagca cggtggcgct ggtcggcgca gcgggccggc tcggcccggc atacgcagtg
      481 acagcggagg ctagggatgc catcaccagc acggcagcgg ctgccagtgc cgggccggaa
      541 cgtatcgaag atttcatggt ccgactcccg gagatgtgcg gtcggattat ttgtatgcct
      601 gtcttacata tgtgtcaatg ggcatccgct tggccggtca atagcaaggc tcacaggcgc
      661 gaagctacgc ctggatctgg gccagcacgg cgtccctggc ccggtcaagg ccagcggcga
      721 gaccgccgag aacgaccgct tcccggccca gaatcgacat ctcaatcggc ggcggcgaga
      781 gcgcgagaag gtcgccaagt agtcgcttgg tcggctcgag cagcaagtcg gcgttgccgc
      841 ccacgccgcc accgatgacg atcaagccgg ggtcgagcac ggcgatcacg gcggcgagcg
      901 cccgcgccag gtgactcgcc tcgtcgtgca cgacgtggat cgcggcgtga tcgcccgacc
      961 tggccgcgtc gaagacctgc tcggccgagg tggcggtcat gccaatgcgc ttggccgccg
     1021 atagcacgcc gtccgccgac gccacggtct caagtatccc gtgctgcttg acattgggcg
     1081 cgagcaactg tccaggcccg tcaccgagcg gcaggaacga gatctcaccg gcccgcccgc
     1141 tcgacccgcg gtaaaggtcg ccgccgatca cgatgcccat tccgatgccg gtgccgaatg
     1201 agacgaagac aaagttgccg gcgcccgcgc ctgccccgtg tttctgttcg gcgaccgtcg
     1261 ccaggttaac gtcgttgtcg atcgagaacc tgggtccgag ctggtccctg agcgcgacga
     1321 ccgagccggg ctcctcccat ccgggaaggt tcggcgcggc catgatgcgg tcggcgtccg
     1381 gctgatagat cccggggaac ccgagcacgg tgtgggtgat gtcggcgagc gtgacgcctg
     1441 cgtcagcggc cgtggcccgt gccagctcgc cgatctgctt ggcgagggcc gaggcggcga
     1501 gcgcccgtac ccggtggtcg gccctggctg tgatcgcgcc ggaaaggtcg gcgaccgcca
     1561 gcctgatcca cgacgctccg acatcgaccg ccatcgcccg tccggcgtca gggcggatct
     1621 cgtagagcct tggcgccctg ccgacctggc cgacgcggtg gccgacctgg ctgatcagcc
     1681 cggcgcgctc caggctgccg aaagcgaggc caatcgtggc cttcgacagg ccggtcgcgg
     1741 cgacaagttg cgacgcggac gccgtaccca gcctcctgat ctgctcgtag accgcgcgtt
     1801 cgttgatgac cctgatgacg gctgttgtgg tcgcgactgg ggtgcttgcg ggcggcgaag
     1861 acttgtccac tgtctcctcc tcgtcgccgc ccggcaccta attcctgcct tgccctccct
     1921 gctgccgcgg gcgggccgcg accgcaaccg ggtcgctcac cgcccggctg tgcttgacgg
     1981 tagcgggcag tacgacccgg taggtcacgc tgatcttacg gggcacaacc gagaattcgg
     2041 tcgtcccgcc ccgatggagc cggtgcgtgt gcacaagctc ccaccgcccg tgaaccaagt
     2101 cctcaagttg cacgacgtca ccgctctgcg ccagcgggat ggagatgacc agcatctccg
     2161 accggccgtg ctcgctccgt agcttcctga ccgtgatcac aggcacgacc gtcgcggacc
     2221 ggcccgggct cggtgaggca gatggactga ggcgcggtga cga
//
LOCUS       DASUBI010000661         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_413392, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000661 DASUBI010000000
VERSION     DASUBI010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413392"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFU72_12770"
     CDS             <1..541
                     /locus_tag="VFU72_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5424411.1"
                     /translation="EPALVQVMFSPGRGESIDNSIAHLITGARRRILLGSMLLNASSL
                     LEALLNVLRQGRIPVRGIYDATQMEQVVQDWSDNRDGGWKIRAFQQVVSTAQMVGKRS
                     TPYTPTSVHDFMHDKIVVVDDTVVTGSYNLSRSATLNAENVLFIESVPLAAAYADYLE
                     SLMRRYGGGVGSPPGEPQP"
     gene            complement(618..1889)
                     /locus_tag="VFU72_12775"
     CDS             complement(618..1889)
                     /locus_tag="VFU72_12775"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5424412.1"
                     /translation="MLVVMRAGSSETDIDEIRRHAQAAGVETNVIHGAGRTVVGLGGS
                     LTSALAAVLADLPGVDEVVDAATLGPPPTRDLRIASIRPLIPPAILLEQLPLPPEGAM
                     LVSRSREEVIRILRGHDDRLFVVVGPCSIHDPAAALDYGRRLRELADELRDELCIVMR
                     VYFEKPRTTVGWEGLINDPHLDGSHAVNEGLYLARRLLLDLIALGVPAGSESLDPISP
                     QFFSDAITWTAIGARTAESQVHRHLASGLSMPVGFKNGTGGGIQLAIDGVIAAAHPHA
                     FLGVTEQGVAAIVATRGNPDCHIILRGGRSGTNYDASSVERTLHALEHAGLPPRLMID
                     TSHGNSNKDYRRQPEVARTVAEQIAGGQPGIVGVLLESFLLDGAQKLEDRSSLVYGQS
                     VTDSCMGWEMTGPVLRDLAAAVRARRSTLGA"
     gene            2195..>2392
                     /locus_tag="VFU72_12780"
     CDS             2195..>2392
                     /locus_tag="VFU72_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5424413.1"
                     /translation="MAKTRIAFVLSGGVSGGSYIAGALDEILRALKSSDQFEVDIISG
                     ASAGATNAALIAHRLCYRDGET"
BASE COUNT          368 a          831 c          829 g          364 t
ORIGIN      
        1 ggagcccgcc ctggtgcagg tgatgttctc gcccggccgg ggcgagtcga tcgacaacag
       61 catcgcccac ctgatcaccg gagcccgccg ccggatcctg ctcggcagca tgctgctcaa
      121 cgccagctcg ttgctcgagg cgctcttgaa cgtattgcgc caggggcgta tcccggtacg
      181 cggcatctac gacgcgaccc agatggagca ggtggtccag gactggagcg acaaccgcga
      241 cggcggctgg aagatccggg cgttccagca ggtcgtgagt accgcgcaga tggtcggcaa
      301 gcgctcgacg ccctacacgc ccaccagcgt gcacgatttc atgcacgaca agatcgtggt
      361 cgtcgacgat acggtcgtga ccggctcgta caacctgtcg cgcagcgcca cgctcaacgc
      421 agagaacgtc ctctttatcg agagcgtccc gctggcggcc gcctacgcgg actacctgga
      481 gtcgctgatg cgtcgctacg ggggcggggt tggctcgcca cccggcgaac cccagccatg
      541 agacgcgtgg atccgggcgc gcccaggccg acgcccgcgc cgaagcggcg gatggggagc
      601 ggtctgttgc tctcgcgtca ggcgcccagc gtggaccgcc gcgcccgcac cgccgccgcc
      661 aggtcgcgca gcaccggccc ggtcatctcc cagcccatgc aggagtccgt gacgctctgg
      721 ccgtagacca ggctggaccg gtcctcgagc ttctgggcgc cgtcgagcag gaagctctcc
      781 agcagcacgc cgacgatccc cggctggccc ccggcgatct gctcggccac ggtgcgcgcc
      841 acctccggct ggcgccggta gtccttgttg ctgttgccgt ggctggtgtc gatcatcagc
      901 cgtggtggca gccccgcgtg ctccagcgcg tgcagcgtgc gctcgacgct gctggcgtcg
      961 tagttggtac cgctgcggcc gccgcgcagg atgatatggc agtcggggtt gccccgcgtg
     1021 gccacgatgg cggccacgcc ctgctcggtc acgccgagga acgcatgtgg gtgcgcggcg
     1081 gcgatcacgc cgtcgatcgc cagctggatg ccaccccccg tgccgttctt gaagcccacc
     1141 ggcatcgaga ggccggacgc aagatggcgg tgaacctgac tctcggcggt gcgcgcgccg
     1201 atggccgtcc aggtaatcgc gtcggagaag aactggggcg agatcggatc gagcgactct
     1261 gagccggccg gaacccccag cgcgatcagg tcgagcagca gccggcgcgc caggtagagg
     1321 ccctcgttga ccgcgtggct accgtcgagg tgcgggtcgt tgatcagccc ttcccagccg
     1381 acggtggtgc gtggcttctc gaagtagacg cgcatcacga tgcagagctc gtcgcgcagc
     1441 tcgtcagcca gctcgcgcag ccgccggccg tagtcgagcg cggcggccgg gtcgtggatc
     1501 gagcagggac cgacgaccac gaagaggcgg tcgtcgtgcc cgcgcaggat gcggatgacc
     1561 tcctcgcggc tgcgactgac cagcatggct ccctcgggtg gcagcggcag ctgctccaac
     1621 aggatcgccg gtgggatgag gggccgaatg ctggcgatgc gcaggtcacg cgtcggcggc
     1681 gggccgagcg tggcggcgtc gaccacctcg tcgacgcccg gcagatcggc cagcactgcc
     1741 gccaaggccg aggtcagcga tccgcccagc ccgaccaccg tgcgcccggc gccgtggatg
     1801 acgttcgtct ccacgccggc ggcctgcgcg tggcggcgga tctcgtcgat gtccgtctcg
     1861 ctgctcccag cccgcatcac caccagcatc gcgtcgtttc ccctcctgca accggctggc
     1921 cgcctggcac cacctcgaca atgcacatga gtacgcaggg tacctcatca gcaggctacg
     1981 cggcaacgca cgtcgacgcc gtcccagatc cgaggcgatc tcttgcatcg gcggtcttcc
     2041 agccttattc tgaagcgaac acccattcat taggcacgtg attgcgattg gcggtggcgt
     2101 cgacggcagt gtttgtacgg tcggacgcgc acccatggcg gacggcgcga ccggcctcgt
     2161 gccgcggaat cgcggcgcga gagaggggga accggtggca aagacgcgca tcgcgttcgt
     2221 gctcagcggg ggcgtctcgg gcggctcgta catcgccggc gcgctcgacg agatcttacg
     2281 cgcgctgaag tccagtgacc agttcgaggt cgacatcatc agtggcgcct cggccggcgc
     2341 gaccaacgcc gcgctgatcg cccacaggct gtgctatcgc gacggggaga cc
//
LOCUS       DASUBI010000662         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_946508, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000662 DASUBI010000000
VERSION     DASUBI010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946508"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1964)
                     /locus_tag="VFU72_12785"
     CDS             complement(204..1964)
                     /locus_tag="VFU72_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5424414.1"
                     /translation="MSESPVKLAGGSDGSPATEDRSPAALRFLVEASRLLTSSLDYTA
                     TIQRIAGLAVPFLADWCTVDILEQGQSRCLAAAHVEPGKEALLWELRRRYPLTPDGPS
                     PGSRALGGQSILYPGFAPGELAGTTRDAEHLRLVTELTPTSAMAVPLIVQGATIGVMT
                     LSLARGQRAFDQTDLQLAEELAGRAAVAVDHARLFQEAQAAIEARDLALAAVDTERRR
                     LLDVFQEAPDLICVLAGPEHVYEFANQRYLQLFPNRRLIGKPMREALPELEEQGIFKL
                     LDQAYRSGESVINHEVRRLLDRDGDGRLEESIFNGVYQPIRGEQGRVDRIVIYGFEVT
                     EQVRARERVEELAAEQQRLYLETTHLLEQRTQFLSIAAHELRTPLTTIKGYIGLLQRV
                     LQRPELDAARIEGLMAQLRGQLGRFEALIADLLDVSRIQQGRVELRRERLDLTGLAVE
                     VLERFRAGGEHSFELEAPEAIVGRWDPLRLDQVFTNLLSNAVKFSAPETTVRLALRRA
                     GDLVEFTIVDQGIGIAPEDLQTIFEPFVRGKVVRQINGTGLGLFITRSLVEQHGGTVA
                     LESAPGLGTTVTVTLPHVPA"
     gene            complement(2168..3175)
                     /locus_tag="VFU72_12790"
     CDS             complement(2168..3175)
                     /locus_tag="VFU72_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5424415.1"
                     /translation="MTTTPAKPINLRYERVRGVHNIPLEEFYTSGHRTCQGCESATVM
                     RGFVKAAGPRTVATGSTGCMYVANTSYMTTPWIIPWMHTQLGAGGSSAVGTAAGLRAL
                     MRKGKIQQEKINVISFCGDIGGGDMGLSGISGAMQTDLDLLVILYDNESAANTDIQAT
                     GMTTYGAQTTFTPPGSKHRIMQRRWKKNVAAMIAVGHPTVRYVGTACASYPPTDFLNK
                     VRKALSVGGPAFLHTFDPCPKGWDYHPRYSGDVGELGIRSGIFPLYEIIDGQVSYTYD
                     ARSTAKGRIPVKEYLMRQGRFAHLIDEDIDYIQKMIDEMWEEWEIPGVAPVKGRLTSR
                     V"
     gene            complement(3206..4444)
                     /locus_tag="VFU72_12795"
     CDS             complement(3206..4444)
                     /locus_tag="VFU72_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424416.1"
                     /translation="MTTTTKPTGEDTGVWESSDLLTGCQAVAEGVRLADVDVIAAFPI
                     RPYTEVMDTLSKIIADGRLDAEYIIADSEHSQFEIAKHASAVNARAFAGSSGTGWCYG
                     FEALVVTATDRLPTLFLVGNRALDDPGAFGVEHNDAMAVRDMGFLLCWVNTPQEALEH
                     VLLGYRIAEDKRVQMPMALAMDGAFLTHSQHMVRIPSTEAVKRFLPQYDLAERRLHPD
                     NPISIAPQVNEDWVMELRRQNWEAARRAPAVIEQAYQEFNGIFGDRYQEPFFFSEYMT
                     DDAEVVLVGLGTVAAPARTAVRRLREQGQKVGYVGLRWFRPFPTNQLRESLSRFKAVG
                     VIDRDFANGSPDDGGILLHEIRSCLYPLRQRPAVVNFLTGLGGRDISIQQCQQMFAET
                     AKAVSDDSRDGFVQWIGVRE"
     gene            complement(4456..>5262)
                     /locus_tag="VFU72_12800"
     CDS             complement(4456..>5262)
                     /locus_tag="VFU72_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEU5424417.1"
                     /translation="KFIGKKTYPYQITTIDGDASLAGLWVFNDDLTHERVLGAIAGID
                     PELISIEAVTGYLRDKTHDEARIDAARAAYDQARQRARTVKAGEGIDWLYETPVLPAW
                     NKFSEGVAVPAVPRHFEIGPRGQSRNESFKRGTTKSQRPVIRFDLCTKCTLCWVECPD
                     QCFDMTSDEYYDVEYEYCVGCGKCAQVCPVKECIVMVDELAFDNYDSPWEHWQRDPSG
                     YVEWAEAKKGTDRVTYPFVTGTGRLVMEGQVIAEGSKPAPKRRPASRVAY"
BASE COUNT          819 a         1712 c         1836 g          895 t
ORIGIN      
        1 aggaaggtgt gccgcgcggc aggacggatc cttcgacagg ctcaggatga caatcttcat
       61 gccgtggcct atcggaagga cgcggttgca ttcgatcacc acaattcttc cacatgtcat
      121 cctgagcgaa gccgaaggat ccgtcttcac gggacactta ccatggtcga tgggtgtacg
      181 aactgtcgtc gcggccggat gagtcatgcg gggacgtgcg gcagcgtcac ggtcaccgta
      241 gtgccgagac cgggcgcgct ctccagcgcg acggtgccgc cgtgctgctc gaccagcgag
      301 cgggtgatga agagccccag gccggtgccg ttgatctggc gcaccacctt gccgcgcacg
      361 aatggctcga agatggtttg cagatcctcc ggcgcgatgc cgatgccttg atcgacgatc
      421 gtgaattcga cgaggtcgcc ggcgcggcgt agggccagcc gcacggtggt ctccggtgcg
      481 gagaatttga ccgcgttgga gaggaggttg gtgaagacct ggtcgagccg aagcggatcc
      541 cagcgtccga cgatcgcttc gggcgcctcc agttcgaagc tgtgctcgcc gccggcgcga
      601 aagcgctcca gcacctcgac cgccagcccg gtgagatcca gccgctcgcg gcgcagctcg
      661 acgcgcccct gctggatgcg cgacacgtcg agcaggtcgg cgatcagcgc ctcgaagcgt
      721 ccgagttggc cccgcaactg agccatcagg ccctcgatgc gcgccgcgtc cagctcaggc
      781 cgttgcagga cgcgctgcag aaggccgatg tagcccttga tcgtggtcag cggggtgcgc
      841 agctcgtggg cggcgatcga gaggaactgg gtgcgctgct ccagcaggtg ggtggtctcc
      901 aggtagagcc gctgttgctc ggcggcgagc tcttccacgc gctcgcgtgc ccgcacctgc
      961 tcggtaacct cgaacccgta aatcacgatc cggtcgaccc gtccctgctc gccgcgaatc
     1021 ggctggtaga cgccgttgaa gatgctctcc tccagccgtc catcgccgtc gcggtcgagc
     1081 agccggcgca cctcgtggtt gatcacgctc tcgccggagc gataggcctg gtcgagcagc
     1141 ttgaagatgc cctgctcctc aagctcgggc agcgcctcgc gcatcggttt gccgatcaag
     1201 cgacggttgg ggaagagctg gaggtagcgc tggttggcga actcgtagac atgctcgggg
     1261 cccgccagga cgcagatgag atcgggcgcc tcttgaaaca cgtcgagcag ccggcggcgc
     1321 tcggtgtcga cggcggccag cgccagatcg cgcgcctcaa tcgccgcctg ggcctcctgg
     1381 aagagccggg cgtggtcaac cgccaccgcc gcccgacccg ccagctcttc ggccagttgg
     1441 agatcggtct gatcgaaggc gcgctggccg cgcgccagcg agagcgtcat gacgccgatg
     1501 gtggcgccct gcacgatcag cggcaccgcc atcgccgagg tcggcgtcag ttcggtgacg
     1561 aggcgcagat gttcagcatc acgtgtggtg ccggccagct cacccggagc gaagccggga
     1621 tagaggatcg actgcccgcc gagcgcgcgc gagccgggcg aggggccgtc gggggtcagc
     1681 gggtagcgac ggcgcagctc ccaaagcagc gcttccttgc cgggttcgac gtgggcggcg
     1741 gccaggcagc gcgactggcc ctgctccagg atatcgacag tgcaccagtc ggccaagaag
     1801 gggacggcga ggccggcgat gcgctggata gtggcggtgt agtcgagcga ggaggtcagg
     1861 agccggctgg cctcgaccaa aaagcgcagg gcagccggcg accggtcctc ggtggccggg
     1921 gacccgtcgc tgccgcccgc cagtttgacc ggagactcgc tcatggtggt gtatcccgcg
     1981 ggaagagccc gcacccccat cccctctccc gcgcacgtct ccgcgcggag aggggagctg
     2041 cccatctagg agagttagcg acacggaagc atcccgaaca cctaaccgtc atctttagcc
     2101 acctcccctc gccccgcggg gctccgtgcg gagaggggcc gtctccgtgc ggggtggggg
     2161 ccgtgcctca gactcggctc gtcaaccgac ccttgaccgg cgccacgccg gggatctccc
     2221 actcctccca catctcgtcg atcatcttct ggatgtagtc gatgtcctcg tcgatcaggt
     2281 gggcgaagcg accctggcgc atcaggtact ccttgaccgg gatgcggccc ttggccgtcg
     2341 aacgggcgtc gtaggtgtag ctcacctggc cgtcgatgat ctcgtagagc gggaagatgc
     2401 cggagcggat gcccagctcg ccgacatcac cggagtagcg cgggtggtag tcccagccct
     2461 tggggcaggg gtcgaaggtg tgcaggaagg ccggcccacc gaccgagagc gccttgcgca
     2521 ccttgttcag gaagtcggtc ggcggatagc tggcgcaggc cgtgccgacg tagcgcactg
     2581 tcgggtggcc gaccgcgatc atcgccgcca cgttcttctt ccagcgccgc tgcatgatgc
     2641 gatgcttgga gccgggcggc gtgaaggtgg tctgcgcgcc gtaggtggtc atgccggtcg
     2701 cctggatgtc ggtgttggcg gccgactcgt tgtcgtagag gatgaccagc aggtcgagat
     2761 cggtctgcat cgcgccggag atgccggaga gacccatgtc gccgccgccg atgtcgccgc
     2821 agaaggagat gacgttgatc ttctcctgtt ggatctttcc cttgcgcatc agggcacgca
     2881 gcccggcggc ggtgccgacc gccgaggagc cgcccgcgcc gagctgggtg tgcatccagg
     2941 ggatgatcca gggcgtcgtc atgtacgagg tattggcgac gtacatgcag ccggtcgagc
     3001 cggtggcgac cgtgcgcggc ccggcggcct tgacgaaacc gcgcatcacg gtggccgact
     3061 cgcagccctg acaagtgcgg tggccggagg tatagaactc ctccagggga atgttgtgca
     3121 cgcccctgac gcgctcgtag cgcagattga tcggcttggc tggggtggtc gtcatcgcat
     3181 caccattcct tcacactgca tcgtgttact cccgcacgcc gatccactgc acgaagccgt
     3241 cgcgcgagtc gtcgctgacg gccttggccg tctcggcgaa catctgctgg cactgctgga
     3301 tcgagatgtc gcgcccaccg aggccggtca ggaagttgac caccgccggc cgctggcgca
     3361 gcggatagag gcaggagcgg atctcgtgca ggagaatccc gccgtcgtcc ggcgagccgt
     3421 tggcgaagtc gcggtcgatc acgccgaccg ccttgaagcg gctcaggctc tcgcgcagtt
     3481 ggttcgtcgg gaaggggcgg aaccagcgta ggcccacgta gccgaccttc tgcccctgct
     3541 cacgcaggcg ccgcacggcg gtgcgcgcgg gcgcggccac ggtgcccagc ccgaccagca
     3601 cgacctcggc gtcgtcggtc atgtactcgc tgaagaagaa cggctcctgg taacgatcgc
     3661 cgaagatgcc gttgaactcc tggtaggcct gctcgatgac ggccggcgcg cgacgtgccg
     3721 cctcccagtt ctggcgccgt agctccatca cccagtcctc gttgacctgg ggcgcgatcg
     3781 agatcgggtt atctgggtgc aggcgccgct cggccaggtc gtactgcggc aggaagcgct
     3841 tgaccgcctc ggtggaggga atgcgcacca tgtgctgcga gtgggtcagg aaggcgccgt
     3901 ccatcgccag cgccatcggc atctgcaccc gcttgtcctc ggcgatgcgg tagccgagca
     3961 gcacgtgctc cagcgcctcc tgcggggtgt tgacccagca gagcaggaag cccatgtcgc
     4021 gcacggccat ggcgtcgttg tgctcgacac cgaaggcgcc ggggtcgtcg agcgcgcggt
     4081 tgccgaccag gaagagggtc ggcaggcggt cggtagcagt gaccaccagc gcctcgaagc
     4141 cgtagcacca gccagtgccg ctgctgccgg cgaaggcgcg cgcgttgacc gcgctggcat
     4201 gcttggcgat ctcgaactgc gagtgctcgc tgtcggcgat gatgtactcc gcgtcgagcc
     4261 gcccatcggc gatgatcttg gagagggtat ccatcacttc ggtgtagggc cggatcggga
     4321 aggcggcgat gacatcgacg tcggccagcc gcacgccctc ggccaccgcc tgacagccgg
     4381 tcaggaggtc gctgctctcc cagacgccgg tgtcttcacc ggttggtttg gtggtagtcg
     4441 tcacgctgcc tcctcctagt acgcgactct gctcgccggg cgacgcttgg gagcgggctt
     4501 gctcccctcc gcgatcactt gcccttccat caccagccgt ccggtgccgg tcacgaaggg
     4561 ataggtcacc cgatcggtgc ccttcttggc ctcggcccac tcgacgtagc cactggggtc
     4621 gcgctgccag tgctcccagg ggctgtcgta gttgtcgaag gccagctcgt cgaccatcac
     4681 gatgcactct ttgaccgggc agacctgggc gcacttgccg cagccgacgc agtactcgta
     4741 ctcgacgtcg tagtactcgt ccgacgtcat gtcgaagcac tggtcggggc actcgaccca
     4801 gcagagggtg cacttggtgc agaggtcgaa gcggatgacc gggcgctggc tcttggtggt
     4861 gccgcgcttg aaactctcgt tgcgcgactg gccgcgcggc ccgatctcga agtggcgcgg
     4921 gaccgccggg acggcgacgc cctcggagaa cttgttccag gccggcagga cgggcgtctc
     4981 gtagagccag tcgatcccct cgcccgcctt taccgtgcgt gcgcgctggc gcgcctggtc
     5041 gtaagcggcc cgggcggcgt cgatacgcgc ctcgtcgtgg gtcttgtcgc gcaggtaacc
     5101 ggtgacggcc tcgatactga tcagctcggg atcgatgccg gcgatggcgc cgagcacgcg
     5161 ctcgtgggtc aggtcgtcgt tgaagaccca caggccggcc aggctggcgt cgccgtcgat
     5221 cgtggttatc tggtagggat aggtcttctt gccgatgaac tt
//
LOCUS       DASUBI010000663         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_9595, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000663 DASUBI010000000
VERSION     DASUBI010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9595"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFU72_12805"
     CDS             <1..252
                     /locus_tag="VFU72_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424418.1"
                     /translation="SYRRWRQTAPVDPRAAQLHLAALLALAAIAATMITDNVVSYLFV
                     MVPAGALAGVSLSTGRWFAETRQAEAIPESQGNTEAVTP"
     gene            249..1088
                     /locus_tag="VFU72_12810"
     CDS             249..1088
                     /locus_tag="VFU72_12810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5424419.1"
                     /translation="MTIELARPARTGERRLTCPICRSPRLSDFWLERNLERCADCGMI
                     FRNPQPPASELGRHYQDAYAEDRVAAGTTNEAGTTIDYARIYVRRLARDLGRAGFVGE
                     RVLDVGAGTGEMSLALREAGAGVVAVEPFAARYCESRGIETYRSLESAAGTGQFDGAV
                     AVDVIEHMDDPLRSLGRIFERLKPGGWCYLATLNTSGLNARLRGANWREARKPDHLFF
                     YTEQSLRQLLDLVGFEDYRRLNWSIRYPHSRPRMFAALALQALRLDSELRVICRRPPE
                     RRR"
     gene            complement(1102..2196)
                     /locus_tag="VFU72_12815"
     CDS             complement(1102..2196)
                     /locus_tag="VFU72_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5424420.1"
                     /translation="MFSARDFGALGNGTADDRPAIQAAINAASKVAGVVYLPDGTYFI
                     GGGLTASGPVKIQGSSASAARILARAGFDLLTISNTSGVEIASLGFTGAYPRATVGYG
                     ISAISASNLTIHDCAFNSIPDTAVSLTNVNQVQVANSVFATTGYSGVRLQDPGAGNLN
                     QYVSVQGCSFSDINCQRAGGHAAVQCHGTAAPSQLFITIKNNSVAAHGVGLGLDSLDS
                     SLVDGNQIVGNQVLGEGIAFTGANNQITNNRVSSCGAAGILQWAIANRSNDGNLISGN
                     TCWDNAQGIAAVCGQSGTVLRNLTMTGNRCYSQSLVHPQNYGIQSYNDGTTSYSWSGV
                     EIANNDLRGNAVGPISLEPPATATVLNNLA"
     regulatory      complement(2518..2598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2823..4001
                     /locus_tag="VFU72_12820"
     CDS             2823..4001
                     /locus_tag="VFU72_12820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424421.1"
                     /translation="MRVLLAHNRYQQPGGEDIVLAAEADLLRRRGHEVVTFTDDNRRI
                     EGMNRLRLAGDTIWSVGSAGALRDLIRSSQPDVVHVHNTFPLISPSIYREARAARVPV
                     VQTLHNYRLICPNSLLHRDGRSCDDCVGRLPWPGIRHACYRESRAQTAVVAAMLAVHR
                     ARHTWSREVGLFIALSDFARRQFVRGGIPAEKLVVKPNFVEPDPGGRLADGGYYLYAG
                     RLDPTKGLAALLAAWEQGGIAAPLHIAGDGPAAAEVRAAAARLPRVRLLGRLDRERLL
                     DQMRSARALVFPSLLYENFPVTIVEAFACGVPVIASRLGAAAEIVGDQQTGLLFEPGD
                     VGDLARTVLSAEANPRLLRRLGAAARADYLANYTGERNYQQLLEIYQRVMGCEPLRTT
                     "
     gene            complement(4210..4299)
                     /locus_tag="VFU72_12825"
     tRNA            complement(4210..4299)
                     /locus_tag="VFU72_12825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4263..4265),aa:Ser,seq:gct)
BASE COUNT          683 a         1426 c         1479 g          730 t
ORIGIN      
        1 tcgtatcgcc gctggcggca gaccgcgccg gtcgacccgc gggcggccca gctccatctc
       61 gcggcgctgc tggccctggc ggcgatcgcc gccaccatga tcaccgacaa cgtcgtctcg
      121 tatctcttcg tcatggtgcc ggccggcgcg ctggcgggcg tgtcgctgag taccggccgc
      181 tggttcgcgg agacgcggca agcggaagcc ataccggaat cgcaagggaa cacggaggcc
      241 gtcactccat gaccatcgaa ctggcgcggc cggcacgcac cggcgagcgg cgtctgacct
      301 gcccgatttg ccgctcaccg cggctctcgg acttctggct ggaacggaat ctggagcgct
      361 gcgctgactg cggcatgatc ttcagaaatc cccagccacc ggcttcggaa ctcggccggc
      421 actatcagga cgcgtacgcc gaagatcggg ttgccgccgg caccaccaac gaggccggca
      481 ccacgatcga ctacgcgcgc atctacgtgc ggcgactggc ccgcgatctg ggccgcgcgg
      541 ggtttgtcgg cgagcgtgtc ctcgatgtcg gcgccggtac cggggagatg tcgctggcgc
      601 tgcgcgaggc gggcgcagga gtcgtggcgg tcgagccgtt cgccgcccgc tattgcgaga
      661 gccgggggat cgaaacctat cgctcgttgg agtccgcggc aggcacgggc cagttcgacg
      721 gcgccgtggc cgtggatgtg atcgagcaca tggacgatcc gctgcggagc cttgggcgca
      781 tcttcgagcg gctgaagccg ggcggctggt gctacctcgc gacactcaac accagcggac
      841 tcaacgcgcg cctgcggggc gcgaactggc gtgaggctcg caagccggat cacctgttct
      901 tttacaccga gcagtcgcta cggcagctac tggacctggt tggatttgag gactaccgcc
      961 ggctgaactg gagcatccgc tacccgcact cgcggccccg gatgttcgcg gcgctcgcgc
     1021 tgcaggcgct gcggctcgat agcgagctac gggtgatctg ccggcgcccg cccgaacgcc
     1081 gccgatgact ggcccggctc gtcaggcaag gttgttcagc accgtcgcgg tcgccggcgg
     1141 ctccaggctg atagggccaa ccgcattgcc gcgcagatcg ttgttcgcga tctccacccc
     1201 gctccagctg tagctggtgg taccgtcgtt gtaggactga ataccgtagt tctgagggtg
     1261 cacgagcgac tggctgtagc aacgattgcc ggtcatcgtc aggttgcgca ggacggtgcc
     1321 gctctggccg cagacggcgg cgatcccctg tgcgttgtcc cagcaggtat tgccactgat
     1381 cagattgccg tcgttggagc ggttggcgat ggcccactgt aggatgcccg ccgccccgca
     1441 actgctgacc cgattgttcg tgatctgatt gttggcgcca gtgaacgcga tcccctcacc
     1501 cagcacctgg ttccccacga tctgattgcc atcgaccagg ctgctgtcca gactgtcgag
     1561 tccgagaccg acgccatgcg cggccacgct gttgttcttg atcgtgatga agagctgaga
     1621 aggtgcggcc gtgccgtgac actgcacggc cgcgtgaccg cccgcgcgct ggcaattgat
     1681 gtcgctgaag ctacagccct gcacgctgac gtattgattc aggttcccgg cgcccgggtc
     1741 ctgcaggcgc acgccggagt agccggtcgt cgcgaagacg gagttcgcca cctgcacctg
     1801 attgacgttt gtcaggctga ctgccgtatc ggggatgctg ttgaaggcac agtcgtggat
     1861 ggtgaggttt gacgccgaga tggcgctgat gccgtagccc acggtcgcgc gcggataggc
     1921 gccggtgaac cccagtgacg cgatctcgac gccgctggta ttgctgatcg tgagcaggtc
     1981 gaagcccgcg cgcgccagga ttctggcggc gctcgcggac gagccctgaa tcttgacggg
     2041 tccgctggca gtcaaaccgc ccccgatgaa gtaggttccg tccggcaggt agacgacgcc
     2101 ggcgacctta ctggcggcat tgatcgcggc ttggatggcc gggcgatcgt ccgccgtgcc
     2161 gttccccagc gcgccgaagt cgcgcgcgct gaacacggag gcgccggacg ggctactcga
     2221 tactgttgtt ggggtcgtgg tgggcgacgg cgttgggctg ggtgtcgatt gcagtccttg
     2281 cttggagccg ccctggcgcc accaacgcgg cttggccgcg gcggaacccg gaaacgcgac
     2341 gcttccggcc aacggaccgg cgatcgccgc gccggcagcc accttccaaa gatctcggcg
     2401 ggtatagcga cgttgccgcg ccgtgaatgg ttggtccccc accaatgcgt gcctttccca
     2461 aaacgagggg tccagcgcca cgaagggcag aagggacggt gcgcggcggc tcacgctcct
     2521 ggcagcccgg cagtctccga acacgagaga cccgcggctt tacgtccccg tctcgcggcg
     2581 ggtttgccct ggtcagttgc tcctagcgta cggacatcga gcggccgccg acagggccga
     2641 tcggggccaa ccggcgtaag tacgcaggtc cggtcctggg agcgaggtcc cagacgaaaa
     2701 tcaccccgat gttccgggcc cgaaacggtg gccacgatag aatcacctgc cggagcgagc
     2761 ggaagcgact ggcccggtgc tcggcctgat cgtccgccgg agaggaaggt ggcgtagcgt
     2821 cgatgagggt cctgctggcg cacaaccgct atcaacagcc gggcggcgag gatatcgtct
     2881 tggcggcgga ggcagacctg ctccggcggc gtgggcacga ggtcgtcacg ttcactgacg
     2941 acaaccgccg tattgagggc atgaaccggc tgcggctggc gggggacacg atctggtcgg
     3001 ttggttcagc gggggcgctc cgggacctga tccgatcgag ccaaccggac gtcgtccacg
     3061 tgcacaacac gtttccgctg atttcgccgt ccatctaccg agaggcgcgc gccgcgcggg
     3121 tgcccgtggt gcagacgctc cacaactatc gtctgatctg ccccaacagc ctgctccatc
     3181 gcgacggccg gagctgtgac gactgtgtcg ggcgtcttcc ctggccgggc atccggcatg
     3241 cttgctaccg cgagtcgcgg gcacagacgg cagtggtcgc ggcgatgctc gccgttcacc
     3301 gcgcgcgcca cacctggagc cgcgaagtcg gtctcttcat cgcgctcagc gacttcgccc
     3361 gtcggcagtt tgtccgggga gggataccag ccgagaaact ggtggtcaaa ccgaacttcg
     3421 tcgagcccga ccccggcggt cgcctcgccg acggtgggta ctacctctac gccgggcgcc
     3481 tggacccgac caaaggtctc gcggcgctgc tggcggcctg ggagcagggt ggcatcgcgg
     3541 cgccgctcca catcgcgggc gatggtccgg ccgccgcgga ggtacgcgcg gccgccgcgc
     3601 gcctgccgag agtacgactg cttgggcgcc tcgaccggga gcgcttgctc gatcagatgc
     3661 gctcggcgcg ggcgctcgtc ttcccgtcgt tgctctacga gaacttcccg gtgacgatcg
     3721 tcgaagcttt cgcctgcggc gtgccggtga tcgcctcccg cctgggtgcg gcagccgaga
     3781 tcgtcgggga ccagcagacc ggcctcctct tcgaaccggg cgacgtcggc gacctggccc
     3841 ggacagtgct ttccgccgag gcgaaccccc gcctactccg tcggctcggc gcagccgcgc
     3901 gcgccgacta cctcgcgaac tacaccggag agcgcaacta ccagcagctc ctcgagatct
     3961 accagcgggt gatggggtgt gagccgcttc gcacgacgta gaggttgtgc gcaggtggca
     4021 gcctgcctac cggattccaa cgataaccct gatcgaaccg agacgcgcct gcggcgacct
     4081 cgatgagatg caacggcgct tgcacttgaa ccgttctgaa gcatcaaatc ggcacgtatg
     4141 gtggcagggc gggccgccat gggaaaagag aacggcgcct gagaccagac tcaggcgccg
     4201 tttcaggggc gggaaggcag ggattcgaac cctgggaccc cgttttgtgg ggtcaatcgc
     4261 ttagcaggcg accccattcg accactctgg caccttcccc ttcgaccggg ctcaggac
//
LOCUS       DASUBI010000664         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_972241, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000664 DASUBI010000000
VERSION     DASUBI010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972241"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFU72_12830"
     CDS             <1..621
                     /locus_tag="VFU72_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5424422.1"
                     /translation="TSHPKYMSDRIIQAVADLPAACEHINLPVQAGDDEVLRRMRRTY
                     TVDYYRERIDFVRRTIPGVTVATDIIVGFPGESEQQFDNTLQLLQDLRCEKVHVAMYS
                     PRPRTLSARWEDDIPRAEKKRRHRAVEDLQRAMSLQRNSGYVGQTLQVLVDGMSKGRW
                     RGRSRGNTLVFFESPVDWKGTLVDVRITESTAWYLLGEPVAAHRAA"
     gene            647..1522
                     /locus_tag="VFU72_12835"
     CDS             647..1522
                     /locus_tag="VFU72_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424423.1"
                     /translation="MAYQFDGRDRAARRKAEVARTHAVKGNWDEAIQTNSEILQTFPR
                     DVEAMNRLGKALIELQRFDEALEAFQTALKIEPQNAIAQRNATRLEQTIAAGMTENAP
                     PASAVPAAEVKSGIFIEEVGKTYVTDLVRPNPDLLFTGIAPADEVELHVQGREVEIRD
                     REGRRLGQLEPRIALRTAQMIESGNRFKAFVVALTGNTVRVILREVFRNPTARLRVSF
                     PRQATIAAPRPYLRETRLTHELEPDVLYDLEEEDEELEEELEEEEGAEEGAEEEDDFE
                     AERDHEEEEEHGIAH"
     gene            complement(1607..1885)
                     /locus_tag="VFU72_12840"
     CDS             complement(1607..1885)
                     /locus_tag="VFU72_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424424.1"
                     /translation="MAKKTTRGTRLNEAQLKAYRARKAETVVETQPAVAEVAAPVVSV
                     RRRTTHGHVTAWGNVADEYQMIRADIRRLLLITALLLIILIALWLMLG"
     gene            complement(1971..2744)
                     /locus_tag="VFU72_12845"
     CDS             complement(1971..2744)
                     /locus_tag="VFU72_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU5424425.1"
                     /translation="MSIITLLSDFGVHDTYVGQMKGVLLGIAPGLSLVDLTHAVPPQD
                     VFEGAFQLSTAWRAFAAGTVHLSVVDPGVGTARRAIAVLVAGHFFVLPDNGLLTLVLQ
                     DAPAEAAVELVTPASAAPTFHGRDVFAPAAARLALAGDLGAVGRSIPPHSLVRLPVPL
                     ISLDAGRVSAPVVSIDHFGNCRTLLPRDALPASAGRLRVRCGALSVVGVSRSYADVAV
                     GQPLALFGSHGGLELAVRDGSAAEEWAIRRSALVEVTAE"
     gene            complement(2747..3415)
                     /locus_tag="VFU72_12850"
     CDS             complement(2747..3415)
                     /locus_tag="VFU72_12850"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU5424426.1"
                     /translation="MSEETDESVRRYTPLPLSAAILAGGRSLRMGRDKALLEIGGRPM
                     LELVAERVAGVADEVFIVAAGRPEYARFGLPLVEDRFPGAGSLGGIYTALTSAAHPFC
                     LVVGCDMPLLSRALLRYLAEQERAYDALVPALAAERSGQGGEETLETLHAIYARSAAP
                     VLEARLRSGRLKIADALEGLRLRRADEATIRQYDPELRSFLNVNTPEQFDRLVALLAE
                     ERAR"
     gene            3653..3916
                     /locus_tag="VFU72_12855"
     CDS             3653..3916
                     /locus_tag="VFU72_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU5424427.1"
                     /translation="MNSSMISKIEKARRYAEEPDRAQIERLQVQFRGDHDIYQVGFSD
                     GHWSCSCHSFSALHNGTCSHIMALERLLGVIAPTETTSPLSLV"
     gene            complement(3967..4227)
                     /locus_tag="VFU72_12860"
     CDS             complement(3967..4227)
                     /locus_tag="VFU72_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424428.1"
                     /translation="MLRIIVRRVRRSLCNYYAALTISAGIVGQPPVAARRAGRNQIVR
                     VQAGQLVGGRWHPGEVVAAGRGGVIVVDLDHSAGRDSTLLPN"
     gene            complement(4236..4562)
                     /locus_tag="VFU72_12865"
     CDS             complement(4236..4562)
                     /locus_tag="VFU72_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU5424429.1"
                     /translation="MKLVFAVVQDYDATQLLEVITKAGFRATQIASTGGFLRVGNTTL
                     MIGVEDYQVNKVVRLIEMNCRERTETVQPEIIGDIHDWYPPDLVEVIVGGATTFVLNV
                     QRFERI"
     gene            4672..5463
                     /locus_tag="VFU72_12870"
     CDS             4672..5463
                     /locus_tag="VFU72_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5424430.1"
                     /translation="MSLKDDDVYALGGLTWQEAAELLARHPVGLLPTGAIEAHGPHLP
                     LDTDVLIARAMARRAAERLVEAGLPALVLPPLSFGVSFVGTCFPGTSPVEPAALEAYL
                     VSLLTQLAPQGYRAIALCNAHLEPAHVATLATAAARATNNTGIPVVVFDKREARWAAR
                     LSDEFRRGARHAGSYETSLVLAASPQAVRRDALEGLPPVWIDLPARLAAGASTFSEAG
                     GALGYFGDPALATAMEGNRLLDALGEMIVELVLAAIDDEEDSGGA"
     gene            5453..6112
                     /locus_tag="VFU72_12875"
     CDS             5453..6112
                     /locus_tag="VFU72_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424431.1"
                     /translation="MEPEITWYTQSAVRVEAGDLRIYVDPYHLPADQPPADVILISHE
                     HGDHLSPADLAQVRTSTTKVFASREAARQLQPPVEVLEPGQSAEYHGLRIRTIPAYTI
                     DKLRPSGEPTHPKESGGLGLVLEVGEHSFYFAGDTDVVPEMAGIGPVDYAFLPVGGHY
                     VMTAEEAAEAARLVQPSIAVPVHYGLAAGTVADAQRFAELVPDQVRVWIPTPPGASRS
                     Q"
     gene            complement(6215..>6969)
                     /locus_tag="VFU72_12880"
     CDS             complement(6215..>6969)
                     /locus_tag="VFU72_12880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5424432.1"
                     /translation="LGRLGAAAAARDQVSTAAVPRADGVPDYDPALFDPHPQSIHIPV
                     LMYHQIGPSAARYVTPLWLFEQEMDWLKDNGYHSITLAQLYDYVLNGGTLPSKPVLIT
                     FDDGTAGQWDAAAALDQRGMHGVFFIPTGFSALSPDQLRSLVARGHEVESHTVSHPSL
                     TQVSDSQLNYELAHSKSWLEAVLGAPVNYLAYPNGDYNSRVIDAAAGAGYRGALAAWG
                     GQDWTPAKRWQEPRVEVSGLLSLSGFAGLVQG"
BASE COUNT         1217 a         2359 c         2290 g         1103 t
ORIGIN      
        1 acatcccatc cgaagtacat gtccgaccgg atcatccagg ctgtggccga cctgcccgcc
       61 gcctgcgagc acatcaacct gccggtgcag gcgggtgacg atgaggtgct gcgccgcatg
      121 cgccgcacct acaccgtcga ttattaccgc gagcggatcg acttcgtgcg tcgcaccatc
      181 ccgggagtga cggtcgccac cgacatcatc gtcggctttc ccggcgagag cgagcaacag
      241 ttcgacaaca ccctccagct gctgcaggac ttgcgctgcg agaaggtcca cgtggctatg
      301 tactcgcctc gtccgcgcac gctctcggcg cgctgggagg acgacatccc gcgcgccgag
      361 aagaagcggc ggcaccgcgc ggtcgaagac ttgcagcgcg cgatgagtct gcagcgcaac
      421 agcggctatg tcgggcaaac gctgcaggtg ctggtcgacg gcatgtccaa ggggcgctgg
      481 cgcgggcgct cacgcggcaa taccctggtc ttttttgagt caccggtcga ttggaaagga
      541 acgctcgtcg acgtgcggat taccgagtcg acggcctggt atctgctcgg cgaaccggtc
      601 gcggcccatc gagccgcctg acggcgccgg tagaggaagg gaacccgtgg catatcagtt
      661 tgacggccgc gatcgcgcgg cacgccgtaa ggccgaggtc gcgcgcacgc acgcggtcaa
      721 gggcaactgg gacgaggcga ttcaaaccaa tagcgagatt ctgcagactt ttccacgcga
      781 tgtcgaggcc atgaatcgcc tgggtaaagc gctgatcgag ctgcagcggt tcgacgaggc
      841 gctggaggcg tttcagacgg cgctcaagat cgagccgcag aacgccattg cccagcgcaa
      901 cgcgacgcgc ctggagcaga ccatcgcggc cggcatgacc gagaacgcgc cgccggccag
      961 cgcggttccc gccgccgagg tcaagagcgg catcttcatc gaggaagtcg gcaagaccta
     1021 cgtcaccgac ctggtgcggc caaaccccga cctgctgttc acgggtatcg cgccagcgga
     1081 cgaagtcgag ctgcatgtgc agggccgcga ggtcgaaatc cgcgaccgtg aagggcggcg
     1141 gctcggccag cttgaaccgc gcatcgcgct gcgcaccgcg cagatgatcg agtcgggcaa
     1201 ccgcttcaag gccttcgtcg tggcgctgac cggcaacacc gtgcgggtca tcctgcgcga
     1261 ggtctttcgc aatccgacgg cccgcttgcg cgtttccttc ccgcgccagg cgaccatcgc
     1321 cgccccgcgc ccctatctgc gggagacgcg gctgacccac gagctcgagc ccgatgtcct
     1381 ctatgacctc gaggaggaag acgaagaact cgaagaggaa cttgaggagg aagagggggc
     1441 agaggaaggg gccgaagaag aggacgattt cgaagccgag cgcgaccacg aggaagaaga
     1501 agagcacggc atcgcccatt gaggcgagta cacgttcgta gccctcgcag aacggctcac
     1561 ctttcggtga gccgttggtc gtttgcagaa gaggatgcgc gagatactac cccagcatca
     1621 gccagagcgc gatcaggatg atcagcagga gtgcggtgat cagcagcagc cggcgaatgt
     1681 cggcgcggat catctggtac tcatccgcga cattgcccca ggccgtcaca tgaccgtgcg
     1741 tcgtccgccg ccggacggac acaaccggcg ccgctacctc ggccacggcc ggctgggtct
     1801 ccaccacggt ttctgccttg cgcgcccggt aggctttgag ctgtgcctcg ttcaggcgcg
     1861 tcccgcgcgt tgtcttcttg gccacgctgc tctgaatcct ctcgaaccac tcgcgtcccg
     1921 cgaatgagcg tgtcggctgt agagacgcaa gtataccagg gaagcgctcc tcactcggcc
     1981 gtgacctcga ccagcgcgct gcgtctgatg gcccattcct ccgcggcgct gccgtcgcgc
     2041 accgccagct ccagccctcc gtggctgcca aagagcgcca gcggttgccc cactgctaca
     2101 tcagcgtacg accggctgac accgacaacg ctcagggcgc cgcagcgcac acgcagtcgt
     2161 cctgccgagg cgggtaaggc atcgcggggc agcagcgtcc ggcagttgcc aaaatggtcg
     2221 attgagacca ccggggccga cacgcggcca gcgtcgaggg agattagcgg caccggcaag
     2281 cggaccagcg agtggggcgg aatcgagcgc ccgaccgcgc ccagatcccc agcgagggca
     2341 aggcgtgcgg ccgccggagc gaacacatcg cgtccgtgga aggtcggtgc ggcgctcgcc
     2401 ggcgtcacca gctcgacggc cgcttcggcc ggcgcatcct gcaacaccag agtgagcaag
     2461 ccgttgtcgg ggagaacgaa gaagtgaccc gcgacgagca cggcaatcgc tcgtcgcgcc
     2521 gtgccgacgc ccgggtcgac caccgacagg tgaaccgtgc ctgcggcgaa ggcgcgccag
     2581 gcggtcgaca gttggaaggc gccctcgaag acatcctgcg gcggtacagc gtgggtcaga
     2641 tcaaccagcg agagtccagg cgcgataccc agcagcacgc ccttcatctg tcccacgtag
     2701 gtatcgtgga cgccgaagtc actgagcagc gtgatgatgc tcacggctat ctcgcccgct
     2761 cctccgccag tagcgcgacc agccgatcga actgctcggg cgtgttgaca ttgaggaacg
     2821 agcgcagctc gggatcgtac tggcggatcg tcgcttcgtc ggcgcgccgc aggcggagcc
     2881 cctccaaggc gtcggcgatc ttgagccgcc ccgagcgtag ccgtgcctcc agcactggcg
     2941 ccgcgctgcg ggcgtagatc gcgtgcaacg tctctagcgt ctcctcgccg ccctggccgc
     3001 tgcgctcggc cgccagggcg ggcaccagag cgtcatacgc acgctcctgc tccgccaaat
     3061 agcgcaggag cgcgcgactc agcagtggca tgtcgcagcc gacgacgagg cagaacgggt
     3121 gcgccgcgga tgtgagcgcc gtgtagatgc cgccgagcga gccggcaccg gggaagcgat
     3181 cctcgaccag cggcaggccg aatcgcgcgt actcgggacg ccccgcggcc acgatgaaca
     3241 cctcgtccgc cacgccggcg acacgctcgg ccaccagctc cagcatgggc cgtccgccga
     3301 tctccagcag cgctttatcg cggcccatgc gcagactccg cccgccagcc aggatcgccg
     3361 cactcaatgg aagaggtgtg tacctacgca ccgattcgtc cgtttcctcg ctcatagccc
     3421 gattgtaccc ctggtttgac agaagccgct gagcgcctat aatcagggta gcgatggctg
     3481 tgagagaagg ggacgacacc gccatgaatg gtcgtccaat caagagccgt gagaggtctt
     3541 agcacccggc agcagtgtcc gggtgctttt ttattgttcg gagtcggcaa cgggtgcttg
     3601 tagctagggt cggtcgcacg cgccgtgcgg ccggccagaa ggagaggccc aggtgaactc
     3661 cagcatgatc agcaagatcg agaaggcgcg acgctatgcc gaggaacccg atcgcgctca
     3721 gatcgagcgg ctccaggtgc agttccgtgg cgaccacgac atctatcagg tcggcttcag
     3781 tgacgggcat tggagctgct cgtgtcactc cttctccgcg ctgcacaacg gcacctgtag
     3841 ccacatcatg gccctggagc gattactcgg cgtgattgcg ccgacggaga ccacctcacc
     3901 gctcagtttg gtctagcaga accgcgagcc ggccgccagt ggcggccggc tcgttcttgt
     3961 tcaacctcag ttcggcagca aggtggaatc tcgcccggcc gaatgatcca gatccaccac
     4021 gatcacccca ccgcgacccg cggcgacaac ttctccagga tgccagcgtc cgccgacgag
     4081 ttgccccgct tgcacgcgca cgatctgatt gcggccggca cgccgagcag ctaccggcgg
     4141 ctggccgacg atacccgcgc tgatggtcaa cgccgcgtag tagttgcaga gggatcgccg
     4201 cacgcgccga acgattatgc ggagcatcgc aaagcctaga tgcgctcgaa gcgctgaacg
     4261 ttgagcacga acgtcgttgc cccgccgacg atgacctcga ccaggtcggg cgggtaccag
     4321 tcgtggatat cgccgatgat ctcgggctgc accgtctcgg tgcgctcgcg gcagttcatc
     4381 tcgatcagcc ggacgacttt gttgacctga tagtcttcga cgccgatcat gagcgtggta
     4441 ttgccaaccc gcagaaagcc gcccgtgctg gcgatctggg tggcccggaa tccggccttg
     4501 gtgatgacct ccaggagctg cgtggcgtcg tagtcctgga cgaccgcgaa caccagtttc
     4561 atctgccgat ctccttcggg cgcacgacgc ggccggcggc ctgcggtatc cgtacgacta
     4621 tagcatactg atttcgcggg cagacgggtg cgaaaccggc gaggaggtgc gatgtcgttg
     4681 aaggacgacg atgtctacgc attgggcggg ctgacctggc aggaagctgc tgagttgctg
     4741 gcgcgtcacc cggtcgggct cctcccgacc ggggcgatcg aggcccacgg tcctcacctc
     4801 cccttggata ccgacgtgct gatcgctcgg gcgatggcgc gccgcgccgc cgagcggctg
     4861 gtagaggccg gtctccccgc gctcgtcctc ccgccgctct ccttcggcgt gagcttcgtg
     4921 ggcacctgct tccccggcac ctcgccggtc gagcccgccg cgctggaggc gtacctcgtc
     4981 tctctgctca ctcaattggc gccccagggc taccgggcca tcgcgctgtg caacgctcat
     5041 ctggaaccag cgcatgtcgc cacgctcgcg acggcagccg cgcgcgccac caacaacact
     5101 ggaattccgg ttgtcgtctt tgataagcgc gaggcacgct gggcggcccg gctgagcgat
     5161 gagtttcgtc gcggcgctcg ccacgccggc agctacgaga cctcgctcgt tctcgcggcg
     5221 agcccccagg ccgtgcgccg tgacgcgctg gaggggctgc cgcccgtctg gatcgacttg
     5281 ccggctcggc tcgcggccgg agccagcacg ttcagcgagg ccggcggcgc gctcggctac
     5341 ttcggcgacc cggcgctcgc caccgctatg gagggcaacc ggctcctgga tgcgctcggc
     5401 gagatgatcg tcgagctcgt gctggcagcc atcgacgatg aggaggattc cggtggagcc
     5461 tgagatcacc tggtacacgc agtcggccgt tcgcgtcgaa gccggcgatt tgcgcatcta
     5521 cgtcgacccc taccacctcc ccgccgatca acccccggcg gacgtgattc tgatcagcca
     5581 cgagcacggc gatcatctct cgcccgccga tcttgcccag gtccgcacgt cgaccaccaa
     5641 ggtcttcgcc agccgcgagg cggcccgtca gttgcagcca ccggtcgagg tgctggagcc
     5701 tggacagagt gccgagtacc acggcctgcg gattcgcacc attccagcct acacgatcga
     5761 caagctgcgc ccatcgggcg agccgacgca cccgaaggag agcggcggcc tcggcctcgt
     5821 cctcgaagtc ggcgagcaca gcttctactt cgcgggcgac accgatgtag tgcccgagat
     5881 ggccggtatc ggcccagtgg actacgcctt cctgccggtc ggcggccact atgtgatgac
     5941 cgccgaggaa gcggccgagg cggcgcgcct ggttcagccg agcatcgccg tgccggtgca
     6001 ctacggcctg gcagccggca ccgtcgcgga cgcccagcgc ttcgccgaac tggtccccga
     6061 ccaggtccgc gtctggatcc caacaccgcc cggcgcgagc cggtcacaat agggcagtcg
     6121 cgcacatcct gttagggcag tcttctgtgt ctgtcctcgc acgctgacca cccattcttg
     6181 tcgacgccga ccaacttgcg gcgtcgtaca cccgctaccc ctgcacgaga ccagcgaagc
     6241 ccgagagcga caaaagcccc gagacctcca cccgcggctc ctgccagcgt ttggccggcg
     6301 tccagtcctg cccaccccag gccgccagcg cgccgcggta gccggcgccg gccgcggcgt
     6361 cgattacgcg actgttgtag tcgccattcg ggtacgccag gtaattgacc ggagcgccga
     6421 gcaccgcttc gagccagctc ttgctgtgcg ccagctcgta gttgagctgg ctatcggaga
     6481 cctgggtgag tgacgggtgc gagactgtgt gcgactcgac ctcgtgaccg cgcgccacca
     6541 gcgagcggag ttgatcgggc gagagcgccg agaaaccggt ggggatgaag aagacgccgt
     6601 gcatgccgcg ctggtcgagc gccgcggccg cgtcccattg gcccgccgtg ccatcgtcga
     6661 aggtgatgag caccggcttc gacggcagcg tgccgccgtt gagtacatag tcgtagagtt
     6721 gcgccagcgt gatgctgtga tagccgttgt ccttgagcca gtccatctcc tgctcgaaaa
     6781 gccacagcgg ggtgacgtag cgagcggcac ttggcccgat ctggtgatac atcagcaccg
     6841 ggatgtggat cgactgtggg tggggatcga acagggccgg gtcgtagtcc ggcacgccgt
     6901 ccgcgcgggg cacggccgcg gttgagacct gatcgcgcgc ggctgccgcc gcgccgagcc
     6961 ggcccagct
//
LOCUS       DASUBI010000665         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_152125, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000665 DASUBI010000000
VERSION     DASUBI010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152125"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFU72_12885"
     CDS             complement(<1..619)
                     /locus_tag="VFU72_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5424433.1"
                     /translation="MSGHSKWAQIKRQKASADYKKGAIFSKLAREIHVAVREGGANPE
                     ANLRLRMAIERAKREAMPNDTIERALNKASGAGADAENWETIVYEGYGPGGVAVLAIA
                     LTDNRNRTASEVRHAFTRHGGTLGETGCVAWQFDTVGQIVLEADGSDPDELALAAIDA
                     GAQDVEPEGAEIVVTTDPAELSQVTEKLKEAGASVAQADIQRVPQA"
     gene            1171..2082
                     /locus_tag="VFU72_12890"
     CDS             1171..2082
                     /locus_tag="VFU72_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEU5424434.1"
                     /translation="MADTEVCPYQLSADFRISSIGPTEGARVAAEREVNPETEQPGAP
                     LVRTVQVIRYVTPLREGGSLPAVVEADDLGTYVLKFRGAGQGAKVLIAELLAGEIART
                     LGLPVPEIVFAQLDPILARSEPDPEIQALIEASAGLNLALDYLPGSFAFDPQDARTLP
                     PELASAIVWFDAYLTNVDRTARNTNMLLWHRQLYLIDNGAALYAQYGGASFPSRARNP
                     FPQIRDHVLLPAASRLAEIDAELSARLTPEVIAGIVATIPESWLDDPAYPGPKAQRAA
                     YAAFLSERLRAPRGFVLEAIDARAALV"
     gene            2060..>2455
                     /locus_tag="VFU72_12895"
     CDS             2060..>2455
                     /locus_tag="VFU72_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEU5424435.1"
                     /translation="MPEPRSFDYAIIRVVPRVERGECLNVGVALFCRVSRFLDLRIAL
                     DRQRLAALAPGLDGPELARQLEHLARIVRGEADAGPIAALSPSERFHWLVAPRSTVIQ
                     TSAVHSGLTADPEATLDHLLRTLVLPPTSR"
BASE COUNT          342 a          929 c          786 g          398 t
ORIGIN      
        1 tcgcctgcgg cacgcgctgg atatccgcct gggccacgct cgcgcccgcc tccttgagct
       61 tctccgtcac ctggctcagc tccgccgggt cggtggtcac cacgatctcg gcgccctccg
      121 gctcgacatc ctgtgccccg gcatcgatcg ccgccagggc cagctcatcg gggtctgacc
      181 cgtcggcctc cagcacgatc tgtccaaccg tatcgaactg ccaggcaaca cagccggtct
      241 cacccagcgt gccgccgtgg cgcgtgaagg cgtggcgcac ttccgaggcg gtgcggttgc
      301 ggttgtcggt cagcgcgatc gccagcacgg cgacgccgcc ggggccgtag ccctcgtaga
      361 cgatcgtctc ccagttctcg gcgtcggcgc ccgcgccact ggccttgttg agggcgcgtt
      421 cgatggtgtc gttcggcatc gcctcgcgct tggcgcgctc gatcgccatg cgcaggcgca
      481 ggttcgcctc cgggttggcc cctccctcgc gcacggcgac gtggatctca cgggcgagct
      541 tgctgaagat cgcgcctttc ttgtagtcgg ccgaggcctt ctggcgcttg atctgtgccc
      601 acttggagtg gcctgacatc gtcgtctgct ccttaccaat tgatcaggtc gggacgcgcg
      661 gcacgcccgg cgccgggccg gcgtggctca agcagcgcgt acgggcggaa aatggaagcg
      721 ggaatgccgc cgaaccccgg ccagccgtta attctcgcac atttcggccc acttttccac
      781 ttcaatcgct cctcaaggaa tgtgctatga tatatatcag ggcgcggcct ggagcatcgc
      841 gcacctgctc attctccgcg ctcctgaatg gaaggcaccg tgaacaccct cggacgctgg
      901 ctcgacgcgc tccgcgccgg tctcgacatc ctgctgggcc gcacgccgcg gcgcgtcccg
      961 gtgctggtgc ccgcgcgccg ccccgcccgc tagcccccag tcagacgacg tttgttacga
     1021 tctgtgggtt gagtacggga gctgtccttg accgctccac gtacacctgt aggggcagac
     1081 ccccgtgtct gccctgctcc cccggcggca tggcaagggg gaaatagggg accgtgggcg
     1141 gcgaacgagc gggtaggcca tagggcaacg atggcagaca cggaggtctg cccctaccag
     1201 ctgtcggcgg atttccgcat ctcctcaata ggaccgaccg aaggagcacg cgtggccgcc
     1261 gagcgcgaag tcaaccccga aacagaacag cccggcgcgc cgctagtccg caccgtacag
     1321 gtgatccgct acgtcactcc gctgcgcgag ggcggttcgc tgccagccgt ggtcgaggcc
     1381 gacgacctcg gcacctacgt gctcaagttt cgcggcgccg ggcagggcgc caaggtgctg
     1441 atcgccgaac tgctggccgg cgagatcgcc cgcaccctcg ggctgccggt gccggagatc
     1501 gtcttcgccc agctcgaccc gatcctggcg cgctccgagc cggaccccga gatccaggcg
     1561 ctgatcgagg ctagcgcggg gctgaacctg gcgctcgact acctccccgg ctccttcgcc
     1621 ttcgatccgc aggacgcgcg cacgctgccg ccggagttgg cctcggccat cgtctggttc
     1681 gacgcctacc tgaccaacgt cgaccgcacg gcgcgcaaca ccaacatgct gctctggcac
     1741 cgccagctct acctgatcga caacggcgcc gcgctctacg cccagtacgg cggcgcgagc
     1801 ttcccgtcgc gcgcccgcaa ccccttcccc cagatccgcg accacgtgct gctgcccgcg
     1861 gccagccggc tggcggagat cgacgccgag ctgagcgccc gcctgacgcc cgaagtgatc
     1921 gcgggcatcg tcgcaaccat ccccgagagc tggctcgacg acccagccta ccccggtccc
     1981 aaagcgcagc gcgccgccta cgccgccttc ctcagcgagc ggctgcgcgc cccgcgcggc
     2041 ttcgtgctgg aagcgatcga tgcccgagcc gcgctcgttt gactacgcca tcatccgggt
     2101 ggtgccgcgg gtcgaacgcg gcgagtgcct caacgtcggc gtggccctct tctgccgcgt
     2161 gtcgcgcttt ctggatctac gcatcgcgct cgaccgccag cggctggccg cgctggcgcc
     2221 cggcctggat gggccggagc tggcgcgcca actggagcac ctggcgcgca tcgtccgcgg
     2281 cgaggcggac gccgggccga tcgccgcgct ctcaccctcg gagcgcttcc actggctggt
     2341 ggccccgcgc agcacggtga tccagacctc ggccgtccac tccggcctga ccgccgaccc
     2401 cgaggccacg ctcgaccacc tgctgcgcac gctggtcctg ccgccgacct cacgc
//
LOCUS       DASUBI010000666         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_623336, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000666 DASUBI010000000
VERSION     DASUBI010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623336"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..2024)
                     /gene="thrS"
                     /locus_tag="VFU72_12900"
     CDS             complement(24..2024)
                     /gene="thrS"
                     /locus_tag="VFU72_12900"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5424436.1"
                     /translation="MPADKLHITLAGREHVVEAGTTAGEALQALAEPPSGADGEIIAA
                     KVNATPRDLAHRLADGDKVEPIGIKSADGLAIVRHSTAHVLAQAVQDLHPDARLGIGP
                     PVENGFYYDFDVNDPFSPEDLKALEQKMRQIIKQGQKFSRRVVSDDEARAELAAEPYK
                     LELIGIKGSPMSASAVSDEESVEVGGTELTIYDNLDPATGQTRWKDLCRGPHVPTTRH
                     IPAFKLMRTGGAYWRGSEQNPQLQRIYGTAWPSRQAQDDYLTMLAEAERRDHRKLGAE
                     LDLFSFPPEIGSGLPVFHPKGGIIRRVMEDYSRRRHEEAGYEFVNTPHITKSTLFETS
                     GHLDWYADGMYPPMEMEGAKYYPKPMNCPMHILIFASRGRSYRELPMRLFEFGTVYRF
                     EKSGVVHGLTRARGFTQDDSHIFCRPDQLADELASLLDFVVGLLRDYGLSEFEAELST
                     RPEKFVGEISEWEVAEAALKHALDASGLPYVVAEGEGAFYAPKIDVHVRDAIGRRWQL
                     STLQVDFQEPGRFGIEYTAADGTRQRPQMIHRALFGSIERFFGILVEHYAGAFPPWLA
                     PVQAVGIPIADHHVPYLESVAARLRARGVRVQVDTGADRMQKKIRTAQQQKVPYMLLA
                     GDDDVAAGAVSFRYRSGEQRNGVPIDEAIEEIVSAIERRIQV"
     gene            complement(2137..>2356)
                     /locus_tag="VFU72_12905"
     CDS             complement(2137..>2356)
                     /locus_tag="VFU72_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5424437.1"
                     /translation="FQIMKGTNPDGSKYADPVKWVSYFRAGEAVHYFPRASYGSQQSL
                     GCVELPYNQAKWIWPYLTYGTLVTVTEA"
BASE COUNT          377 a          796 c          761 g          422 t
ORIGIN      
        1 ctacccagcc ccgtgctagc cgctcagacc tggatgcgcc gttcgatcgc gctgacgatc
       61 tcctcgatgg cctcatcgat cggaacgccg ttgcgctgct cgccgctgcg gtacctgaac
      121 gacaccgcgc ccgccgcgac gtcgtcatcg ccggccagca gcatgtaagg caccttctgc
      181 tgctgagccg tcctgatctt tttctgcatc cggtcggcgc cggtgtcgac ctgaacccgc
      241 accccgcgcg cccgcagccg tgcggccacc gactccaggt acggcacgtg gtgatcggcg
      301 atcgggatcc cgaccgcctg aaccggcgcc agccacggcg gaaacgcgcc agcgtagtgc
      361 tcgaccaaga tcccgaagaa ccgctcgatc gacccgaaca gcgcccggtg gatcatctgc
      421 ggccgctgcc tggtcccgtc ggcggcggtg tactcgatgc cgaaccggcc gggctcctgg
      481 aagtccacct gcaaggtgga cagctgccac cggcggccga tggcatcgcg gacgtggacg
      541 tcaatcttcg gcgcgtagaa ggcaccctcg ccctcggcca ccacgtaagg cagccccgac
      601 gcgtcgagcg catgcttgag cgccgcctcg gccacctccc actccgagat ctccccgacg
      661 aacttttccg gcctggtgga cagctcagcc tcgaactcgg agagcccgta gtcgcgcagc
      721 agcccgacga cgaagtcaag caggctcgcc agctcgtcgg cgagctggtc aggtcggcag
      781 aagatgtgcg aatcgtcctg tgtgaacccg cgggccctgg tcaagccgtg caccacaccc
      841 gacttctcga accggtacac ggtgccgaac tcgaacagcc gcatcggcaa ctcccgatag
      901 gaccgcccgc gcgacgcgaa gatcaagatg tgcatcgggc agttcatcgg cttcgggtag
      961 tacttcgccc cttccatctc catgggcggg tacatgccgt cggcgtacca gtccaggtgg
     1021 cccgaggtct cgaacagggt cgacttggtg atgtgcggtg tgttgacgaa ctcgtagccg
     1081 gcttcttcgt gcctgcgccg cgagtagtcc tccatcaccc gcctgatgat cccgcccttg
     1141 gggtggaaca caggcagccc gctgccgatc tcgggaggga acgagaatag gtcgagctcg
     1201 gcgcctagct tgcgatgatc gcgccgctcg gcctcggcga gcatcgtgag gtagtcgtcc
     1261 tgcgcctggc gcgacggcca cgcggtgccg tagatccgct gcagctgcgg gttctgctcg
     1321 ctgccacgcc agtaggcgcc gccggtgcgc atcagcttga acgcggggat gtgcctggtg
     1381 gtcggcacgt gcgggccacg gcacaggtcc ttccagcggg tctggccggt ggctgggtcc
     1441 aaattgtcgt agatggtcag ctcggtgccg ccgacctcga ccgactcctc gtccgacact
     1501 gcgcttgccg acatcggcga gcccttgatc ccgatcagct caagcttgta gggctcggcc
     1561 gcgagctccg ctcttgcctc gtcgtccgac accacccggc ggctgaactt ctggccctgc
     1621 ttgatgatct ggcgcatctt ctgttcgagc gctttaagat cttcggggct gaaaggatcg
     1681 ttcacatcga agtcgtagta aaagccattc tcgaccggcg ggccaatgcc gagcctggcg
     1741 tcggggtgca gatcttgcac cgcctgggcg agcacgtgcg cggtggaatg gcgcacgatg
     1801 gcaagcccgt ccgctgactt gatgccgatc ggctcgacct tgtcgccatc ggccagacgg
     1861 tgcgccaggt cgcgcggagt ggcgttgacc ttagcggcga taatctcgcc gtcggcgccg
     1921 gacggcggct cggcgagcgc ctggagtgcc tcgccagccg tcgtgccagc ttccaccacg
     1981 tgctcgcgtc cggcgagtgt gatgtgaagc ttgtcggcag gcacggctaa ctccttaggt
     2041 tgtcgtctgg ctaataaccg cgaaattcga tgctagcggg cgcacggcag tgagcgcaca
     2101 cgagttatgc ggcactgcga cgggtcaggc ggccggctac gcctccgtga ccgtcaccaa
     2161 cgttccgtac gtgagatatg gccagatcca cttggcctgg ttgtagggca gttccacgca
     2221 gcccaggctc tgctgggagc cgtaggaggc acgcgggaag tagtgcacgg cctcgccggc
     2281 ccggaagtag gacacccact tgaccgggtc ggcgtacttg ctgccgtccg ggttggtgcc
     2341 cttcatgatc tggaag
//
LOCUS       DASUBI010000667         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1108645, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000667 DASUBI010000000
VERSION     DASUBI010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108645"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..555
                     /locus_tag="VFU72_12910"
     CDS             115..555
                     /locus_tag="VFU72_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5424438.1"
                     /translation="MTTIFTIGFTQKSAETFFGLLQHAGVRRVLDARLHNTSQLAGFA
                     KQDDLRYFLDAIGRIGYRHEPLLAPSEAIFSDYRKKAITWPEYESRFHALLQERAIES
                     RLEPAELGGACLLCSEATPQRCHRRLVAEYLASHWPDVEIVHLV"
     gene            760..2001
                     /locus_tag="VFU72_12915"
     CDS             760..2001
                     /locus_tag="VFU72_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5424439.1"
                     /translation="MIPRRLFAGASQLLALCLILALVASCSLSNNSSGKAGASAATLT
                     TTAAAGTATSSPTTPSSAATPMSNGSTAAGTSSGAASLEQAVINVVNTSKAAVVEITN
                     EQTPSSQFNQPIPTGLGSGFIYDNQGHILTNNHVVAGAQALLVTLPDGRSFKGNDVKV
                     VGTDPQTDLAVLQITGNNLPVIKLGDSSQLQVGQWLVAMGYALGLEGQPTVTSGVASA
                     LGRTVQEPSPPSQSGASGQSNQAGPFLFDVIQTDAAINPGNSGGPLTTLNGEVVGINT
                     LVAGMAEPGVPAQGIGFAIAINTAKKIADQIVKTGHAVHPFVGIAYVPLNPALAAQLN
                     TSVSAGILVQDVVSGSPAAQAGLTKGDIITQVDGTDLKTDSAFAQMIEGHHPGDSVSL
                     TVVRNGQPQQVKVTLGTRPTS"
     gene            complement(2282..3205)
                     /locus_tag="VFU72_12920"
     CDS             complement(2282..3205)
                     /locus_tag="VFU72_12920"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5424440.1"
                     /translation="MTNALPVYPLGLPALDALVRELLRRLADEAPGRVQSLYLFGSHV
                     DGSAAASSDLDLFVVTHGELAPGEADLIRALGQQLSFGSNELDLLPIGSAHLLRDGHW
                     RLEANSRLVAGDDLRPSLPAEPFDSYLRRYTHAPYAYMSQVLRGVARLDYPLDYPDPG
                     GEFYGYDHEPRPPAPRDPAARDIRALVATACWIASLSVGLLAGQKVPSKSRAPAAYAE
                     HVGDEWTGFVATVYRSGKERWGYLAPADPAARAELRALCRDMLAFENAYLLRYRDYLL
                     AELQAGPEARRVAAERLRDWVRYPEVPDADG"
BASE COUNT          564 a         1240 c         1175 g          515 t
ORIGIN      
        1 ccctattcac tacagtgcgg cggattccgc aagtcggacg cggtcatgtc gcctgccgtc
       61 gtgctgatgc gatcgtaggt gtacccacga gtgccgctgt accagaggaa ggccatgacg
      121 acgatcttta cgatcggctt cacgcagaag agcgctgaga cgttcttcgg cctgctgcag
      181 cacgcgggcg tgcgccgggt gctcgatgcg cggctgcaca acacctcgca gctggccggc
      241 ttcgccaagc aggacgacct gcgctacttc ctcgatgcca tcggccgcat cggctaccgc
      301 cacgagccgc tgctggcgcc cagcgaggcg atcttctcgg actaccgcaa gaaggccatt
      361 acctggccgg agtacgagtc ccgcttccac gccctgcttc aggagcgggc gatcgagtcg
      421 cggctcgaac cggcggagct gggcggcgcc tgcctcctct gcagcgaggc cacgccgcag
      481 cgctgccacc ggcgactggt ggccgaatac ctcgccagcc actggccgga cgtcgagatc
      541 gtgcatctgg tgtagcgccc agcccagagg ctcagcacct ggaaatctcc ccacgaccgc
      601 ccgcctgacc aacgggatgg catccattgt gcttcctgcg acactaacag tccgggccgg
      661 atgactcaaa tcggtggatc gctccggcgc cggacacggc accgttcaac cttcgccagc
      721 gggcggccac cccgctcacg aagcatccca ggaggcatga tgattccaag acggctcttc
      781 gcgggggcga gccagttgct cgcgctctgt ctgattctgg ccctggtggc tagctgttcg
      841 ctgtcgaaca attcttccgg caaggccgga gccagcgccg ccacactgac cacgaccgcg
      901 gcggccggaa ccgccaccag cagccctacg actccgtcca gcgcggccac gccgatgtcg
      961 aatggctcga ccgcggcggg cacgtccagc ggggccgcca gcctggagca ggccgtcatc
     1021 aacgtggtca acacgtccaa ggcggccgtg gtcgagatca ccaacgagca gacgcccagc
     1081 tcgcaattca accagcccat ccccaccggc ctcggctccg gcttcattta tgacaaccag
     1141 ggccacattc tgaccaacaa ccacgtggtc gcgggcgcgc aggcgctgct ggtgacgctc
     1201 cccgatggcc gctccttcaa aggcaacgac gtcaaggtgg tcggcaccga cccgcagacc
     1261 gacctggccg tgctgcagat caccggcaac aacctgccgg tcatcaagct cggcgactcc
     1321 agtcagctcc aggtcggcca atggctggtg gccatgggct acgcgctcgg gctggagggc
     1381 cagccgacgg tgacttcagg cgtggccagc gcgctggggc gcacggtgca ggagccgagc
     1441 ccgccgagcc agtcgggcgc gtccggccag tcgaatcagg ccggcccctt cctcttcgac
     1501 gtgatccaga ccgacgcggc gatcaacccc ggcaactcgg gcggcccgct gacgacgctc
     1561 aacggtgagg tcgtcggcat caatacgctg gtggccggca tggccgagcc gggtgtcccg
     1621 gcgcagggca tcggcttcgc catcgcgatc aacacggcca agaagatcgc cgaccagatc
     1681 gtcaagaccg gccacgcggt ccatccattc gtcggcatcg cctacgtgcc gctgaatccc
     1741 gccctcgccg cgcagctcaa cacctcggtc agcgccggca ttctcgtcca ggacgtggtc
     1801 tccggctcgc cggccgccca ggcgggcctg accaagggcg acatcattac ccaggtcgac
     1861 ggcacggatc tgaagaccga ctcggccttc gcgcagatga tcgagggcca ccatcctggc
     1921 gacagcgtgt cgctgacggt ggtgcgcaac ggccagccgc agcaggtgaa ggtcacgctg
     1981 ggcacgcggc ccacctcgta gggtcctcac cccaacgggt ggccctcacc ccccatcccc
     2041 tctcccagtg cgctgggaga ggggcgctgc ccttcgacaa ggatgggtgc tattcgagga
     2101 cggatccttc gactgcgctc agagcctgcc ctgagcttgc cgaagggatg acagggggag
     2161 gggatgaggc ctaccgggcg aggagcgtcc ttgtcgtcag cccctctctt accctgtcat
     2221 cctgagcgca gtcgaaggat ccgtcttacc ttccgtccca tccttgtcga aggccatcgg
     2281 ttcacccgtc ggcgtcgggc acctccgggt agcggaccca gtcgcgcagg cgctcggcgg
     2341 cgacgcggcg cgcttcgggg ccggcctgaa gctcggccag caggtagtcg cggtagcgca
     2401 ggagataggc attctcgaag gcgagcatgt cgcggcagag cgcgcgcagc tcggcgcgcg
     2461 cggccggatc ggccggcgcc aggtagcccc agcgctcctt gccgcttcga tacaccgtgg
     2521 cgacgaagcc ggtccactcg tcgccgacgt gctcggcgta ggcggccggc gcccggctct
     2581 tcgagggcac cttttgcccg gccagcaggc ccacgctcag gctggcgatc cagcaggcgg
     2641 tggccaccag cgcgcggata tcgcgggcgg cggggtcgcg cggcgcgggt ggccgcggct
     2701 cgtggtcgta gccatagaac tcaccgccgg ggtccgggta gtcgagtggg tagtccagcc
     2761 gcgccacgcc gcgcagcacc tgggacatgt aggcgtaggg cgcgtgggtg taacgccgca
     2821 ggtaggagtc gaagggctcg gccggcaggc tgggccgcag gtcgtcgccc gccaccagcc
     2881 ggctgttggc ctccaggcgc cagtggccgt cgcgcagcag gtgggcgctg ccgatcggca
     2941 gcagatcgag ctcgttggag ccgaacgaga gctgctggcc cagcgcgcga atcaggtccg
     3001 cctcgccggg cgccagctcg ccgtgcgtca cgacgaagag gtcgagatcg ctgctggcgg
     3061 ccgcgctgcc gtcgacgtgg ctgccgaaga ggtagaggct ctggacccga cccggcgcct
     3121 cgtcagccag ccggcgcagc agctccctca ccagcgcgtc gagcgccggg aggccgagcg
     3181 ggtagacggg aagggcgttc gtcatcggtc cgtgcctcgg ttcggtggga acgagatgcg
     3241 tcccaggcgt gttgccttcc ctgccgacat cgcggcgcgt gacgcgcggg ggaattacgg
     3301 cggcccaacg aattcgtcaa ccgccgtaat gcccccgcga cattggacac cagcgcccgg
     3361 cgggacgcaa caatcccgct ccccgcgcct cacccatcct tcggccagcg cgaggtcagc
     3421 gaggccagcg gcagttcggc gctggtgacc gactccgccg ggtccagcag cggtgccgcc
     3481 gggtggaagc cgtc
//
LOCUS       DASUBI010000668         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_734275, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000668 DASUBI010000000
VERSION     DASUBI010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734275"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFU72_12925"
     CDS             complement(<1..236)
                     /locus_tag="VFU72_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424441.1"
                     /translation="MQRGTSHVLNAALVLCALLAISIVQVSAAANPYDWFDLGGNDPL
                     NFEATGSNGDYWTVSNLMGGVGWNNTQIISNQYA"
     gene            1209..3005
                     /locus_tag="VFU72_12930"
     CDS             1209..3005
                     /locus_tag="VFU72_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5424442.1"
                     /translation="MSSQARDLATQEQELIAEVSPARLMETTAAIARWERLSGTPDEA
                     KAFDYIQEELDKAGLTTHRYSHPALVSWPEAAALTLRTASGELSLPAKTHAFAAPAPE
                     GIEGELVDAGRGTAADLANVDLRGKIALIDGIIAPNHNLRVEQAGAIASIWIAGQRLH
                     ERALSPVWGTPTPETAGLLPKTPSVSVLATEGARIKEALAHGEVRARLTTRVYQAWHQ
                     LPIVTGDLAPSRPGPEADEFVLFSGHVDSWYYGAMDNGTANATQLEVARILAGHRDEL
                     RRGVRFAFWSGHSHARYAGSAWYADNFWHDLHEHCVAHVNIDSVGGMGASILSEGNTM
                     EEVRAFVSDAVEPVAGERLAARRYGRAGDQSFWGHGIPAMLMSLSAQPAENVDPALAA
                     LHHQISGGESKSGGLGWWWHTPEDTIDKIDPALLERDARIYTLLVYRLATTKLLPFDY
                     AAVVADLQQNAARLQAAAGQHFDLAPLVDSLDELRQGVERLAASLRARGGDVDDAQAA
                     RVNQALMDLGRALIPVDYTATGMFDHDLAVPTSPLPALQAAARLADLESDQSAYQFQR
                     TRLTRERNRAVYGLRQANQAVARALAALGGER"
     gene            3008..3763
                     /locus_tag="VFU72_12935"
     CDS             3008..3763
                     /locus_tag="VFU72_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5424443.1"
                     /translation="MAIDLTGRIAIVTGAAQGIGAATARKLTERGAFVVGVDIEGDKL
                     QAVMAQLEGLAVVADVTDPEADRQLVQRVHLAYEHVDILVNVAGGTLGAGRGNEAVTI
                     EEWQRVLALNMSAPFYLAKAAAPIMMAQRSGRIISVGSGAGRSHSRSKVVPYAAAKAG
                     LMGLMRQLAVELAPYGITCNTVSPGFILTERGEADWARRTPEEQREEMLTIAAGRLGR
                     PEEIANAIAFVASDEAAYIAGQTIMVDGGHWMF"
     gene            3914..5749
                     /locus_tag="VFU72_12940"
     CDS             3914..5749
                     /locus_tag="VFU72_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424444.1"
                     /translation="MVSTEPAERSSPISASSSAPAGTALGEHPGRVPLVVRVGVVGDM
                     ATTTDLEQLRERVRASLDLIARTAGELAATARAAFAPEPPRLRVISSLAAGAEQLVAR
                     EGLDLGYTLECPLPFERARYARTFAQPEAAAYQTLLERAAAVLELDGTDATADERAAA
                     GHAANRVMLVHSDVLLAIWDGAEGSQSAAARLVDQAEQLAIPVVWIAGAAPHAVSLLV
                     SGENERQVLPLERLREQLEPALLPPLGDDAERLRDYFAETYPRWSVLGRVWPFFHDLF
                     GRSRLRRPRLRGITPETAAADWQRDWETAPRLPAEVTRQIDRGLEPHFIWSDTLASHY
                     VSMYRSSFILIGLLAVLSVFLGVFGAHAHWAEEIPYFEATRQVAAVGALLLIMLLWQL
                     GTRHRWHARGIDYRFLAEELRQLRYLAPLGRVPAFSRPRIHDAADDPDKTWMGWQARS
                     ITRAMSLVDARITSGYLEACHALLRDGLIGGQATYHEAAAQRYERFEQRLHRMGFLTF
                     FGALVLGVISFVIELPGVTWLEVVLPALGATLGAIRSQAELERLAKRSEAMAQYLEQL
                     SAQLPAAAGSSSSLADTAELATEALAAEVSDWRIVFQSKPLELPG"
BASE COUNT          915 a         2023 c         1982 g          895 t
ORIGIN      
        1 tccgcatact gattgctaat gatctgcgtg ttgttccaac ccacgccgcc catgaggttc
       61 gacactgtcc agtagtcacc atttgatcca gtagcctcga aattgagcgg gtcgttccca
      121 cccaggtcga accagtcata tggatttgcg gcagccgaaa cctgtacgat ggagattgcg
      181 agcaaggcgc agagtacgag agccgcattc aagacgtgcg aggttccgcg ttgcatcaga
      241 tccctcccgc cccactgtct aaggaaatga tccgatcagc cttccgaata ccgcccgctg
      301 ttcgtttagc tggaccacgg cgctgttatc actgcataga caatcctctc ctttacgatg
      361 tgtgtcggcg tagcatagtt cactcatcta aaattcaggc ggtaccgtga taacctcacg
      421 agagaatatg gcaatgtatg ctgaagccat atcgcgcgtt agcgcggcac agcgtcaatt
      481 gggatcgtat ccgttcgcgg aaactcgctt ctgttgttag cattcgtccg cctttagggg
      541 gtggaaacaa aagattcgcg cgcgttgcca ccggcggttc cgacgcttgt cgattctaac
      601 tgtcaaatcc tcccgggctg ggtcacggga tggcgaatct gctttcaaat agttcggcgg
      661 atacttgttt gcctccagtt tgtacgtgtt tcctttagac gataatacta tgcgcagcgt
      721 atcgcggcgg ctcatctatc tgcgctatcg atcctcacta ctgcgatgcc tatattctag
      781 cggagggggg ggtatacgtc aatacctctt tttgggacat tattgtcctg cattggcaat
      841 ctcacgcgca ccgatgctgc gccgtcggca atcgctctcg ccctgtggcc ctgtcggggt
      901 gccccacacg ttcttgcgag gcgcggagaa tggcgtcagt ccaacgtgat cgtggcgccc
      961 aggcgcgcgg ttcactggtt cagcaacgag aaagtcggcc agcgaacgcg gttacggcac
     1021 cgaccggcgt cagcagcgca tctgagaggg cctggtcgta cgccgggcca tcgagagtca
     1081 cggtggcccg ggcaaccttc gcaaaactct gtgaggcacc cgccctgtcg caccagcttg
     1141 acgccgtgcc acacgacgtg ctacgaaagc cacacgacaa tccggcaatg caccccgaag
     1201 gagcgagcgt gagcagccag gcgcgcgacc ttgccaccca ggagcaggag ctgatcgcgg
     1261 aggtatcccc cgcgcggctg atggagacga ccgcggccat cgcccgttgg gagcgactct
     1321 ccggcacgcc ggacgaagcc aaagccttcg actacattca ggaggagtta gataaggcag
     1381 gactcactac ccaccgctac agccacccgg cgctggtctc ctggccggag gcggccgcgc
     1441 tgaccctgcg gaccgccagc ggcgagctct cgctgccggc caagacgcat gccttcgccg
     1501 ccccggcgcc cgagggcatc gagggcgagc tggtcgacgc cgggcgcggc acggcggcgg
     1561 atctggcgaa cgtcgacctg cgcggcaaga tcgcgctgat cgacggcatc atcgcgccca
     1621 accacaacct gcgcgtcgag caggccggcg ccatcgcctc gatctggatc gccggccagc
     1681 ggctgcacga gcgcgccctc tcgccggtct ggggcacgcc gacccccgag accgccggtc
     1741 tgctgcccaa gacgccctcg gtctcggtgc tggccaccga gggcgcgcgc atcaaggagg
     1801 ccctggcaca cggtgaagtg cgcgcccggc tgacgacgcg cgtctaccag gcctggcacc
     1861 aactgccgat cgtgaccggc gacctggcgc cttcccggcc cggaccggaa gcggacgagt
     1921 tcgtcctctt cagcggccac gtcgactcct ggtactacgg cgcgatggat aacggcacgg
     1981 ccaacgccac ccagctcgaa gtggcgcgca tcctggccgg gcaccgcgat gagctgcggc
     2041 gtggggtgcg cttcgccttc tggtcgggcc actcgcacgc gcgctacgcc ggctcggcct
     2101 ggtacgccga caacttctgg cacgacctgc acgagcactg cgtggcccac gtcaacatcg
     2161 actcggtcgg cggcatgggc gccagcatcc tctccgaggg gaacaccatg gaggaggtgc
     2221 gcgccttcgt cagcgacgcg gtcgagccgg tggccggcga gcgcctggcg gcgcggcgct
     2281 acgggcgagc cggcgaccaa tccttctggg gccacggcat cccggccatg ctgatgtcgc
     2341 tctcggcgca gccggccgag aacgtcgacc cggcgctggc cgcgctgcac catcagatca
     2401 gcggcggcga gagcaagagc ggcggcctgg gctggtggtg gcacacgccg gaggacacca
     2461 tcgacaagat cgatccggcg ctcctggagc gcgacgcgcg catctacacc ctgctcgtct
     2521 accgcctggc gacgacgaaa ctcctcccct tcgactacgc cgccgtcgtc gctgacctcc
     2581 agcagaacgc ggcgcggctg caagccgccg ccgggcagca tttcgacctg gcgccgctgg
     2641 tagactcgct cgacgagctg cggcagggag tcgagcgcct ggccgcgtcg ctgcgcgcgc
     2701 ggggcggcga cgtcgacgat gcgcaggcgg cgcgggtgaa ccaggcgttg atggacctcg
     2761 gccgcgcgct gatcccggtc gactacaccg cgaccggcat gttcgaccac gacctggcgg
     2821 tgccgaccag cccgttgccg gcgctgcaag cggcggcgcg gctggccgac ctggagtccg
     2881 atcagagcgc ctaccagttc cagcgcacgc gcctgacgcg cgagcgcaac cgcgccgtct
     2941 atgggctgcg gcaggccaat caggcggtcg cgcgggcgct ggccgcgctc ggaggggagc
     3001 gataacgatg gcgatcgacc tgaccggacg gatcgcgatc gtgaccggcg cggcacaagg
     3061 aatcggtgcg gctacggcgc gcaagctgac cgagcgcggc gccttcgtgg tcggcgtcga
     3121 tatcgagggc gacaagctcc aggcggtgat ggcccagctc gaagggctgg cggtcgtggc
     3181 cgacgtgacc gaccccgagg cagaccgcca gctggtgcaa cgcgtgcatc tggcctatga
     3241 gcacgtcgac atcctggtca acgtagccgg gggcacgctg ggcgccgggc gcggcaacga
     3301 ggcggtcacg atcgaggagt ggcagcgggt gctggcgctg aacatgagtg cgcccttcta
     3361 cctggccaag gcggccgcgc caatcatgat ggcgcagcgc tcggggcgga tcatctcggt
     3421 cggctccggc gccgggcgca gccacagccg cagcaaggtc gtcccctacg cggcggccaa
     3481 ggccgggctg atggggctga tgcggcagct ggcggtcgag ctggcgccct acggcatcac
     3541 ctgcaacacc gtctcgcccg gcttcatcct gaccgaacgc ggcgaggccg actgggcgcg
     3601 gcgcacgccc gaggagcagc gggaggagat gctgacgatc gccgccggcc ggctggggcg
     3661 gccggaggag atcgccaacg ccatcgcctt cgtcgcctcc gacgaggcgg cctacatcgc
     3721 cggtcagacg atcatggtcg acggcggcca ctggatgttc tagcgggcgc ggccctcatc
     3781 cccccgcccc cctctcccaa tgctgggaga agggggagta gctacaggag cagagagatg
     3841 ttccccccac gatctcccct ctcccagcat tgggagaggg gctgggggtg agggcccgta
     3901 aggggagtac acgatggtca gcaccgagcc cgcggagcga tcgagcccaa tctccgcgtc
     3961 ctccagcgcg ccggccggca cggcgctcgg cgagcacccg ggccgggtac cgctcgtcgt
     4021 gcgcgtcggc gtcgtcggcg acatggcgac cacaaccgac ctggagcaac tccgtgagcg
     4081 cgtgcgcgcc agcctggacc tgatcgcgcg cacggccggc gagctggcag cgacggcacg
     4141 cgcggccttc gcccccgagc cgccccggct gcgggtgatc tcctcgctgg ccgcgggcgc
     4201 cgagcaactg gtggcgcgcg aggggctgga cctcggctac acgctggagt gcccgctgcc
     4261 gttcgagcgc gcgcgctacg cgcgcacgtt cgcccagccg gaggcggccg cgtaccagac
     4321 gctgctggag cgggccgcgg cggtgctgga gctggacggc accgacgcca cggcggacga
     4381 gcgcgccgcc gccggccacg ccgccaaccg ggtcatgctg gtccatagcg acgtgctgct
     4441 cgccatctgg gacggcgcgg agggctcgca gagcgcggcg gcgcggttgg tggatcaggc
     4501 cgagcagctg gccatcccgg tcgtttggat cgccggcgcc gcgccccacg ccgtcagcct
     4561 gctcgtctcg ggcgagaacg agcgccaggt gctgccgctc gagcgcctgc gcgagcagtt
     4621 ggagccagcc ctgctgccac cgctcggcga cgacgccgag cgcctgcgag actatttcgc
     4681 cgagacctac ccgcgctggt cggtgctggg ccgcgtctgg cccttcttcc acgacctgtt
     4741 cggccgctcg cggctgcgcc ggccgcggct gcgcggcatc acccccgaga ccgccgccgc
     4801 cgactggcag cgcgactggg agaccgcgcc gcgcctgccc gccgaggtga cgcgccagat
     4861 cgaccgcggg ctggagccgc acttcatctg gtccgacacg ctggcctcgc actacgtcag
     4921 catgtaccgt agctcgttca tcttgattgg cctcctggcg gtcctctcgg tcttcctggg
     4981 cgtcttcggc gcgcacgccc actgggcgga ggagatcccg tacttcgagg cgacgcggca
     5041 agtcgcggcg gtcggcgcgc tcctcctgat catgctgctc tggcagctgg gcacgcgcca
     5101 ccgctggcac gcgcgcggga tcgactaccg cttcctggcg gaggagctgc gccagttgcg
     5161 ctacctggcg ccgctgggcc gcgtgccggc cttctcccgc ccgcgcatcc acgacgccgc
     5221 cgacgacccg gacaagacct ggatgggctg gcaggcgcgc tcgatcacgc gcgccatgag
     5281 cctggtcgac gccaggatca cgtccggcta cctggaagcc tgccatgcgc tgctgcgcga
     5341 cggactgatc ggcggccagg ctacctatca cgaggccgcc gcccagcgct acgagcgctt
     5401 cgagcagcgg ctgcatcgca tgggcttctt gaccttcttc ggcgcgctgg tcctcggcgt
     5461 gatctccttc gtcatcgaac taccgggcgt cacctggctc gaagtggtgc tgccggcgct
     5521 gggcgccacg ctgggggcga tccgcagcca ggcggagctg gagcggctgg ccaagcgctc
     5581 ggaggcgatg gcgcagtacc tcgagcagct cagcgcacaa ctgcccgcgg cggccggctc
     5641 ctcctccagc ctggccgaca ccgccgagct ggcgaccgag gcgctggcgg ccgaggtcag
     5701 cgactggcgc atcgtcttcc agtccaagcc gctggagctg ccggggtagt gacgcgcgcg
     5761 gcctatcgag taggttgggc ggcgcgtgga gcacggatcc ttcgactgag ctcac
//
LOCUS       DASUBI010000669         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_62053, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000669 DASUBI010000000
VERSION     DASUBI010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62053"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1055
                     /locus_tag="VFU72_12945"
     CDS             198..1055
                     /locus_tag="VFU72_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5424445.1"
                     /translation="MSLAAVALILGAAGIHASWNLLVKRVNGGPPFLWLFSALSALVY
                     APLAAIVLLVERPALGPAQWLFLLGSGLMQVVYFLLLQHGYRVGDLSVVYPLARGTGP
                     LLATIGAIIILGERPGPLALLGGLLIIGGVFALAGGASLLRQRPDIGVIFGVLVGVAI
                     ATYTLWDKHAVSALDIPPLLLTWDQMLVLTLALSPLALRRQPEIARLWRCNRRQAFVI
                     ALFCPLAYILVLTALRFTAVSYVAPAREISILIGTFMGARLLSEGALRQRLAAAGVMV
                     VGIGLLVLA"
     gene            complement(1018..1599)
                     /locus_tag="VFU72_12950"
     CDS             complement(1018..1599)
                     /locus_tag="VFU72_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424446.1"
                     /translation="MMTAAERWVRTVEDARVFVRSAGLCLIFKAGPGSRYPALWDVVD
                     APDSQPGEGGHGPRDMLVWRLKNELPTCYPDEVFYGKLSGGRAMLCTLERLADLYRAQ
                     HREAGALSRDAQRLLALIAGRPISNKDLRLDSGFHTPDTRARFERALQELQVSFQIAR
                     VDVEPDTWFPFAAVYPGIAGSFTPAPAARSRRP"
     gene            complement(1604..2125)
                     /locus_tag="VFU72_12955"
     CDS             complement(1604..2125)
                     /locus_tag="VFU72_12955"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU5424447.1"
                     /translation="MPVEVVLLLTGAYLVHSPAGLLGALALLATADFAGTLALYAAAR
                     RGGGLLRRWLRRAGHPRLLRRFAGWRRRLGRHDLALICLGHLLPGLRMSVAPGAGLLD
                     FPLGIMLLGTLAGALLWVGIPLGLGYHFHAEIARLVRLAGEADRATVLAVVAAIAVLA
                     LAWWLHRRLEGER"
     gene            complement(2405..>3160)
                     /locus_tag="VFU72_12960"
     CDS             complement(2405..>3160)
                     /locus_tag="VFU72_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5424448.1"
                     /translation="EVLVSRWNDVPLTESLVTEHAHELAAVICEPLQRSLIPAPGFLA
                     ALREITQRHGVLLIFDEVVTGFRLAYGGAQERYGVVSDLATYGKAIAGGYPLAAVAGR
                     AEIMAVTDASRKGKAPYAQIGGTLSGNPVAAVAGLATLDILRQPGSYDRLYGTTERLK
                     SGLEELFSEHGVPAQVCGEGGLFQVFFNDQPVTDYPGVLASDRAKGRRFGLECVDRGL
                     WLNPGEKFYVSLAHDTEDVEKTLDIFADALDAM"
BASE COUNT          446 a         1127 c         1135 g          452 t
ORIGIN      
        1 aacaggctga gttctcatct ccagcctgtt tcaacaggct tcgtctgcta gcgcggcggc
       61 ttcagccccg cgcggtcgtg gtgcgcggac atgctcgcac gaaacccacc ccatcccccg
      121 ttcacaaccc cccgccgacg cggtaggatg cgccgggacg aacgaggcgt aaccgcatgg
      181 agcgggagga gtgagccgtg tcgctggccg cggtggcctt gatcctcggc gcggccggca
      241 tccacgcgag ctggaacctg ctggtcaagc gggtcaacgg cggtccgccc ttcctctggc
      301 tcttcagcgc gctctcagcc ctcgtctacg ccccgctggc ggccatcgtg ctgctggtcg
      361 agcgtccggc gctcggtccg gcccagtggc tcttcctgct gggcagcggg ctgatgcagg
      421 tcgtctactt cctcctgctg cagcacggct accgcgtcgg cgacctctcg gtcgtctacc
      481 cgctggcgcg cggcaccggc ccgctgctgg cgacgatcgg cgcgatcatc attctgggcg
      541 agcggcccgg cccgctggcg ctgctcggcg gcctcctgat catcggcgga gtcttcgcgc
      601 tggccggcgg ggccagcctg ctgcgccagc gcccggatat cggggtcatc ttcggcgtgc
      661 tggtcggggt ggcgatcgcc acctacaccc tgtgggacaa gcacgcggtc agcgcgctgg
      721 atatcccgcc gctgctcctg acctgggacc agatgctggt gctgacactg gcgctcagcc
      781 cgctggcgct gcgccgccag ccggagatcg cgcggctctg gcgctgcaac cggcggcagg
      841 cgttcgtcat cgcgctcttc tgcccgctgg cctatatcct ggtcctgacc gcgctgcgct
      901 ttaccgctgt cagctacgtg gccccggcgc gcgagatcag catcctgatc ggcaccttca
      961 tgggggcgcg gctcctctcg gaaggggcgc tacgccagcg gctggccgcg gccggcgtta
     1021 tggtcgtcgg gatcgggctg ctggtgctgg cgtgaagctg ccggcgatgc cggggtagac
     1081 cgccgcgaag gggaaccagg tgtccggctc gacatcgacc cgggcgatct ggaaggagac
     1141 ctgcagctcc tgcagagcgc gctcgaagcg ggcgcgtgta tccggcgtgt ggaagccgct
     1201 gtccagccgc aggtccttgt tcgagatcgg ccgcccggcg atcagcgcca gcagccgctg
     1261 ggcgtcgcgg ctgagcgcgc cggcctcgcg gtgctgcgcg cggtagaggt ccgccagccg
     1321 ctccagggtg cagagcatgg cccggccgcc gctcagcttg ccgtagaaga cctcgtccgg
     1381 atagcacgtc ggcagctcgt tcttcagccg ccagaccagc atgtcgcgcg ggccgtggcc
     1441 accctcgccc ggctgcgagt cgggcgcatc gaccacgtcc cagagcgcgg ggtagcggct
     1501 gcctggacca gccttgaaga tcaggcagag gccggcggag cgcacgaaga cgcgcgcgtc
     1561 ctcgacagtg cggacccagc gctcagccgc ggtcatcatg cgactatcgt tccccctcca
     1621 gccggcggtg cagccaccag gcgagcgcca gcacggctat cgccgccacc accgcgagca
     1681 ctgtggcgcg gtccgcctca ccggccaggc gcaccagccg ggcgatctcg gcgtggaagt
     1741 gataacccag gccgagcggg atgcccaccc agaggagcgc gcccgccagc gtgcccagca
     1801 gcatgatacc caacgggaaa tcgagcagcc cggcgccggg cgcgacgctc atgcgcaggc
     1861 cgggcagcag gtggccgagg cagatcaggg ccaagtcgtg gcggccgagc cggcgccgcc
     1921 atcccgcgaa gcgccgcagc agccgcggat ggccggcgcg ccgcagccag cggcgcagca
     1981 gcccgccgcc ccgacgcgcc gcggcgtaga gcgccagtgt gccggcgaaa tcggccgtcg
     2041 ccagcagcgc cagggcgccg agcagccccg cgggagaatg caccagatac gcgccggtca
     2101 gcagcagcac cacctcgacc ggcacgatgg tcgggatgtc cagctcggcg gcgaagacga
     2161 ccagagcggc cagcgccagg ccgtagtgct gcagcaggtg ggtgattggg ggctcctccg
     2221 ggcggcgtga ggggctgtcg tgtggcgatt ggcgccggcg ggcgtgcggg ggagggcaga
     2281 cacggaggtc tgcccctaca ggaaatacgc cgtgccttgc ctgtgggggc ggacccccgt
     2341 gtccgccctg ttccgcttac ggcgaggcag cgagaacacg gcagagcgcc ccctacgcct
     2401 cgtgctacat agcgtccagg gcgtcggcga agatgtcgag ggtcttctcg acgtcctcgg
     2461 tgtcgtgcgc cagcgagacg tagaacttct cgccgggatt gagccagagg ccgcgatcga
     2521 cgcactccag cccgaagcgg cgccccttgg cgcggtcgga ggccagtacg ccggggtagt
     2581 cggtcaccgg ctggtcgttg aagaagacct ggaagagtcc gccctcgccg cagacctgcg
     2641 ccggcacccc gtgctccgag aagagctctt cgaggccgga cttcagccgc tcggtggtgc
     2701 cgtagagccg gtcgtagctg cccggctggc gcaggatgtc gagcgtggcc agcccggcga
     2761 cggccgccac cgggttgccg ctgagcgtgc cgccgatctg ggcgtagggc gccttgccct
     2821 tgcgcgacgc atccgtgacc gccatgatct cggctcgccc ggcgacggcg gccagcgggt
     2881 agccgccggc gatcgccttg ccgtaggtgg ctagatcgga caccacgccg tagcgctcct
     2941 gcgcgccgcc gtaggccagc cggaagccgg tgaccacctc gtcgaagatc agcagcacgc
     3001 cgtggcgctg ggtgatctcg cgcagcgcgg ccaggaagcc gggcgccggg atgagcgagc
     3061 gctgcagcgg ctcgcagatc accgcggcca gctcgtgcgc gtgctcggtc accagcgact
     3121 cggtcagcgg cacgtcgttc cagcgcgaga ccagcacctc
//
LOCUS       DASUBI010000670         6988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_760641, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000670 DASUBI010000000
VERSION     DASUBI010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760641"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VFU72_12965"
     CDS             complement(<1..1314)
                     /locus_tag="VFU72_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5424449.1"
                     /translation="MEQPRVPATESRDWRPAGISADDILNSALDAIIAIDGGGTVREW
                     NRSAEELFGYPREVALGRELDALIIPPELRARYRAGLAHFLLTGEGPALRRRLEVPAM
                     RADGERFPVELTVAHLATPHGPLFIGSLRDISARRQAERRLAAQYTVNRILADAAGLG
                     ETMDAIVRALCAALDWDAGVIWQTDAEAHLLRCLTLWHQPAHPLAAFLDLSRQTTFAP
                     GVGLPGRVWAAHAPQWIRNVEEDDNFPRAAAARSEGLHGALGFPIVSRGVVLGVAEFF
                     SRAIRAPDEEMLATLGALGSQIGQFVERTQTEQALSGIQRELSARLAEINRLHAVSAR
                     LALSEDLRPLFGEVLAAVVALQDADMGMLKLYDPAQDDLFTVASSGFSDDYLAQVERV
                     PRGTGACGVALAERRPVVVSDIALEPSFAPYRAVARLAGYRAVYST"
     gene            1494..2879
                     /locus_tag="VFU72_12970"
     CDS             1494..2879
                     /locus_tag="VFU72_12970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424450.1"
                     /translation="MARLQLAIRRGEIVTGSQTVLADLGISGGKIVQIGGDFEAEREY
                     DALGKLILPGVIDAHVHLTLAPGREHIEPHWVDDFTSGSAAAAAGGVTTVGNMCALAP
                     GEPPIEGLARYTELAARTAFVDVILHPILSEITPQVLEQIPLLLDHGHNSIKYFMSTP
                     RFDTQVMGYLEATQRAGDSGLISLIHCEDYAILESATQRLVAGGATGFAQYPESRPVI
                     AEAVATARAVAFAEATGAPVYIVHLSSERALEACVAAQARGLPVYVETRPFYLHLTRE
                     RFDEPEAAKYVGQPPLREQRDLDAIWAGIQQGVVNTVCTDHAPWSLAAKLAPEHTLDH
                     LRPGAENLQVMLPLLYSEGVRGGRISLARMVEVLSTNAAKLFGIYPSKGTIAPGSDAD
                     LVVFDPHLTRTIDGAMLQSNADYSVYEGWEVTGWPVLTLRRGEVLYQDGEVHGRPGSG
                     EVLRRVATMPL"
     gene            complement(3290..3859)
                     /locus_tag="VFU72_12975"
     CDS             complement(3290..3859)
                     /locus_tag="VFU72_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424451.1"
                     /translation="MPQLRQQSQSPKQTQQRRLFIGARAALVAGLIVGIVVVGSQLFI
                     SAIYSERDSRALVAAMAGPAGTLSSSIITGLATILALMLTMLSLSQNLNQHLSAAFYT
                     RIKRIAVVAIADMITGIVLLLILSSPIQTATQQSRQASRLEVTLTYYALIALSGIVAG
                     LFVAVIVMLYNAVSTVVEVIRPDRFNDEG"
     gene            3966..5243
                     /locus_tag="VFU72_12980"
     CDS             3966..5243
                     /locus_tag="VFU72_12980"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEU5424452.1"
                     /translation="MANETVFMMDTSAIKYGLGATREVGWDMRAQGSRRVLVVTDPRL
                     AQGESVATVLNALRAEGIDAVLFDRVRVEPTDRSFQDAIDAANESRADGFVAVGGGSV
                     MDTAKAANLYSTYPADFLTYVNAPIGQGQPVPGPLKPLIAVPTTAGTGSETTGVAIFD
                     LTERHVKTGIAHRALRPLLGIVDPLNTRTLPRMVAACSGLDVLSHAIESLTALPFDQR
                     PAPEHPRMRPAYQGANPISDIWSTRAIEMVATNLLPAIEEPDNDEARGQMLLAAAFAG
                     IGFGNAGVHLPHGMSYPVSGMVRNFQPEGYPDDHPIVPHGMSVILNAPAVFRFTGPAN
                     PERHLLAARLLGADMRGAEAADAGVILADALLALMRRLDLPNGLSGIGYGEADIDKLV
                     EGTLPQHRVTKLSPRPAGPEELRALFRDAMRYW"
     gene            5311..5763
                     /locus_tag="VFU72_12985"
     CDS             5311..5763
                     /locus_tag="VFU72_12985"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5424453.1"
                     /translation="MLETSETFTINAPIEAVWSYASDMRNWAANMPFFDTFQQLGEKE
                     SRWTLKPKLGPFTRNVRMLVTITEWEPPSHIGFTLHGETDPVDGNGSFDARVLGPERT
                     DVTLALRLDSHGPLAPMMEGLARPVLPRMARSFAQSLTRDIERGAGEG"
     gene            complement(5926..>6988)
                     /locus_tag="VFU72_12990"
     CDS             complement(5926..>6988)
                     /locus_tag="VFU72_12990"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424454.1"
                     /translation="AFDAWLSAAAGDASGFWFVSLFAKLFPVPFVWGEYAAAGILDAQ
                     AARDYFATPGLDRASLGWAGSAFAWGGGQLADAWPNTADEDTYRRVRTSAVETLLVGG
                     ALDTSTPPQVAANELLPYLPNGQQIVLPGLGHTGSFFAEQPEAGSRLVNTFFDSGQVD
                     ASLYTPIAVDFTPRITLTALAKVVAGAMIGLALVVVASLLWMPRRVRKQGSFAPKTSA
                     ALRSLYPIVLGLGGWSLGALIVLTFWPTVPLDSELLGVVAIGVPIGLGTYWAWVHRDW
                     LPRLKTGGFAVAVGSGLLGAWLGFNATAGLPALITTIVGSAAVTNLSLIVVDIWRERE
                     VQQRLDAATPPPAFPAASA"
BASE COUNT         1106 a         2494 c         2401 g          987 t
ORIGIN      
        1 cgtgctgtag acggcacggt agccggccag ccgggcgacg gcgcgatagg gcgcgaagct
       61 cggctccagc gcgatgtcgc tcacgaccac cggccggcgc tccgccagcg ccaccccgca
      121 ggccccggtt ccgcgtggaa cccgctcgac ctgcgccagg tagtcgtcgg agaatccgct
      181 actggcgacc gtgaagagat cgtcctgcgc gggatcgtag agcttcagca tgcccatatc
      241 ggcgtcctgc agggcgacga cggcggccag cacctcgccg aagagcggtc gcagatcctc
      301 gctgagcgcc aggcgggcgc tgaccgcgtg cagccggttg atctcggcca ggcgcgccga
      361 cagctcccgc tggatgccgc tcagcgcctg ctccgtctgc gtgcgctcca cgaactggcc
      421 gatctggctg ccgagcgcgc cgagcgtcgc cagcatctcc tcgtccggcg ctcggatcgc
      481 acggctgaag aactcagcca cccccagcac cacgccgcgg ctcacgatcg ggaagccgag
      541 cgcgccgtgc agcccctcgc tgcgcgcggc ggccgcgcgc gggaagttgt cgtcctcttc
      601 gacgttgcgg atccactgtg gcgcgtgcgc ggcccacacg cgccccggca gcccaacccc
      661 cggcgcgaag gtcgtctggc gcgagaggtc gaggaaggcg gccagcgggt gagccggctg
      721 gtgccagaga gtcaggcagc gcagcaggtg agcctcggcg tcggtctgcc agatgacccc
      781 ggcatcccag tcgagcgcgg cgcagagggc gcgcacgatc gcgtccatcg tctcgccgag
      841 ccccgcggcg tctgccagga tgcggttcac ggtgtactgc gccgccagtc gccgctcggc
      901 ctgacgccgc gcgctgatat cgcgcagcga gccgatgaag agcgggccgt gtggggtcgc
      961 caggtgcgcg acggtcagct cgaccgggaa gcgttcgcca tcggcgcgca tggctggaac
     1021 ctcgagcctc cggcgcaggg ccggcccctc gcccgtgagc aggaagtggg ccaggccggc
     1081 gcgatagcgc gcgcgcagct ccggtgggat gatcagcgcg tccagctccc gcccgagcgc
     1141 cacctcacgc ggatagccga agagctcctc ggcgctccgg ttccactcgc gcaccgtccc
     1201 gccgccgtcg atcgcgatga tggcgtcgag cgcggagttg aggatgtcgt cggcgctgat
     1261 gccggccggg cgccagtccc ttgactcagt ggccggtacc cgtggctgtt ccatagtcgc
     1321 tgcctcgtgc tccccgctca tgatggtaag cacgtgatgc cggcgtcgct ggtcagattg
     1381 tccgatcatt atcggtcagc aatgtaggac gcgcaaccgc ctgattgacg gatcgcccgc
     1441 gctggagcta aataatccgg tgagccggcg tggacgggaa gggatggacg gcgatggcgc
     1501 gactccagct tgcgatccgg cgcggcgaga tcgtcaccgg gagccagacg gtgctggccg
     1561 acctcggcat ctccggcggc aagatcgtgc agatcggcgg cgacttcgag gccgagcgcg
     1621 agtacgacgc gctgggcaag ctgatcctgc ccggcgtgat cgacgcccat gtccacctca
     1681 ccctggcgcc ggggcgcgag cacatcgagc cgcactgggt cgacgacttc accagcggct
     1741 cggccgccgc cgcggccggc ggcgtcacca ccgtcggcaa catgtgcgcg ctggcgcccg
     1801 gcgagccacc catcgaggga ctggcccgct acaccgagct ggccgcccgc accgccttcg
     1861 tcgacgtcat cctgcacccg atcctgagcg agatcacgcc ccaggtgctg gagcagatcc
     1921 ccctgctgct cgaccacggc cacaacagca tcaagtactt tatgtcgaca ccacgcttcg
     1981 acacgcaggt gatgggctat ctggaggcga cccagcgcgc cggcgactcc ggcctgatct
     2041 cgttgatcca ttgcgaggac tacgccatcc tggagagcgc cacccagcgg ctggtggccg
     2101 gcggcgcgac cggcttcgcc cagtaccccg agtcgcgtcc ggtcatcgcc gaggccgtcg
     2161 ccaccgcgcg agcggtggcc ttcgccgagg cgaccggcgc cccggtctac atcgtccacc
     2221 tctcgtcgga gcgggcgctg gaggcctgtg tcgcggcgca ggcgcgcggt ctgccggtct
     2281 acgtcgagac gcgccccttc tacctgcacc tgacgcgcga gcgcttcgac gagccggagg
     2341 cggccaagta cgtcggccag ccgccgctgc gcgagcagcg cgatctggac gcgatttggg
     2401 ccggcatcca gcagggcgtc gtcaacaccg tctgcaccga ccacgcgccc tggtcgctgg
     2461 cggccaagct ggcgcccgag cacacgctcg accacctgcg cccgggcgcc gagaacctgc
     2521 aagtcatgct gccgctcctc tactccgagg gcgtgcgcgg cgggcgcatc agcctggcgc
     2581 gtatggtcga agtcctctcg accaacgccg ccaagctctt cggcatctac cccagcaagg
     2641 gcaccatcgc ccccggcagc gatgccgacc tggtcgtctt cgacccgcac ctgacgcgca
     2701 ccatcgacgg cgccatgctc cagtcgaacg ccgactactc cgtctacgaa ggctgggagg
     2761 tcaccggctg gccggtgctg accctacgcc ggggcgaggt gctctatcag gacggcgaag
     2821 tccacggccg ccccggcagc ggcgaggtcc tgcgacgggt ggcgacgatg ccgctgtagc
     2881 cggagatgag cggttccgca ttgccccaga gtacgtgcgc cagggatcgc gcggcactaa
     2941 agtgaccgcg ctgagtccac gaagcccact gaaggggctg aggattcacg ctccacgtcg
     3001 cacgccaacc cggaacgtga gcgagagacc cacgttgggg agatccttcg gctgcggcct
     3061 cagagcctgc cctgagcttg tcgaagggat gacaggggta gaggatgccc ttttccctgt
     3121 catcctgagc cgcagcgaag ggtctcccca tgccccgcct attgccgccg ttcggtacat
     3181 ggagcgcgga agctcagccc cttcagtggg cttcgtctgg ttagcgcggt tggctttagc
     3241 cccgcgcgac gggtggctca gatgctccat gtgcgccaac ttgcatgccc tacccctcat
     3301 cattgaaccg atcggggcgg atgacctcga ccacggtgga gacggcgttg tagagcatca
     3361 cgatcaccgc cacgaagagc ccggccacga tgccggagag cgcgatcagc gcgtagtagg
     3421 tgagcgtgac ctccagccgc gacgcctgcc gcgactgctg cgtcgcggtc tggatcggcg
     3481 agctcaggat caacaggagc acgatgccgg tgatcatgtc ggcgatcgcg accaccgcga
     3541 tgcgcttgat gcgggtgtag aaggcggcgc tgaggtgttg attgaggttc tgcgacaggc
     3601 tgagcatggt cagcatcagc gccagaatgg tggccagccc ggtgatgata ctggaggaga
     3661 gcgtgccggc cggaccggcc atggccgcca ccagcgctcg gctatcgcgc tctgagtaga
     3721 tggcgctgat gaagagctgc gagccgacga ccacgatgcc gacgatcagc ccggccacca
     3781 gcgcggcccg cgccccgatg aagaggcggc gctgttgggt ctgcttcggc gattgtgact
     3841 gctgccttag ctgcggcacc ggcgctccct ccgccggcgc cgtgcaaaat cgctgccgcc
     3901 tccgcgcggc actggcttgc tagaatgccg gtggctgggc cggcgcatga aaaggagcga
     3961 gtgccatggc gaacgagacg gtcttcatga tggatacgtc cgcgatcaag tacggcctgg
     4021 gggccacgcg cgaggtcggc tgggacatgc gcgcccaggg ctcccggcgc gtcctggtcg
     4081 tgaccgatcc gcgcctggcg cagggagaga gcgtcgccac cgtgctcaac gcgctgcgcg
     4141 cggagggaat cgacgccgtg ctcttcgacc gcgtgcgcgt cgagccgacc gaccgctcct
     4201 tccaggacgc catcgacgcc gccaacgaga gtagagccga cggcttcgtg gcggtcggcg
     4261 gcggctcggt gatggacacc gccaaggccg ccaacctcta ctcgacctac cccgccgatt
     4321 tcctcaccta cgtcaatgcg ccaatcgggc agggccagcc ggtgccgggg ccgctcaagc
     4381 cgctgatcgc cgttcccacc accgccggca ccggcagcga gacgaccggc gtggccatct
     4441 tcgacctgac cgagcgccac gtgaagaccg gcatcgccca ccgcgcgctg cgcccgctgc
     4501 tcggcatcgt cgacccgctc aacacccgca cgctgccccg gatggtggcg gcctgctccg
     4561 ggctcgacgt gctcagccac gccatcgagt cgttgaccgc cctccccttc gaccagcgcc
     4621 cggcgccgga gcatccccgt atgcgtccgg cctaccaggg cgccaacccg atcagcgaca
     4681 tctggtcgac ccgcgccatc gagatggtcg ccacgaacct gctcccggcc atcgaggagc
     4741 cggacaacga cgaagcgcgc ggccagatgc tgctggcggc ggccttcgcc ggcatcggct
     4801 tcggcaacgc cggcgtccac ctgccgcacg gcatgtccta cccggtctcc ggcatggtgc
     4861 gcaacttcca gccggagggc taccccgacg accacccgat cgtgccccac ggcatgagcg
     4921 tcatcctcaa cgcgcccgcc gtcttccgct tcaccggccc ggccaacccg gagcgccacc
     4981 tgctggccgc gcgcctgctg ggcgccgaca tgcgtggcgc cgaagcggcc gacgccgggg
     5041 tgatcctggc cgacgcgctg ctcgcgctga tgcgtcgcct cgacctgccc aacggcctct
     5101 cgggcatcgg ctacggcgag gccgacatcg acaagctggt cgagggcacg ctgccgcagc
     5161 accgcgtcac gaaactttcg ccgcgcccgg ccggccccga ggagctgcgc gccctcttcc
     5221 gcgacgccat gcgctactgg tagaggcggc ggcacgcggc gcgcgagcac gcgcctgggg
     5281 cggtgacgag cgggaggaga cgagggcacg atgctggaga cgagcgagac gttcacgatc
     5341 aacgcgccga tcgaggcggt ctggagctac gccagcgaca tgcgcaactg ggccgcgaac
     5401 atgcccttct tcgatacctt ccagcagctc ggcgagaagg agtcgcgctg gacgctcaag
     5461 cccaagctcg gacccttcac ccgcaacgtg cgcatgctgg tcacgatcac cgagtgggag
     5521 ccgcccagcc acatcggctt cacgctccac ggcgagaccg atccggtcga cggcaacggc
     5581 agcttcgacg cgcgcgtgct cggccccgag cgcaccgacg tgacgctggc gctgcggctt
     5641 gactctcacg gcccgctggc gccgatgatg gaggggctgg cgcggcccgt gctgccgcgc
     5701 atggcccgct ccttcgccca atccctcacc cgcgatatcg agcgtggcgc gggcgaggga
     5761 tagcggagtc cgcgaaattc acccgcggtg tgggcgcgcc gtgccgagtc cgaagcaggg
     5821 gtgggatccg agccgaacgg ggccacccct gctgaacggg ctgggtctgg ccgcgacgga
     5881 tgccgagcaa gcgcaccaac ctcgaacttc gcgcgcccgc tgtcatcagg cactggcagc
     5941 cgggaacgcc ggcggaggcg tggccgcatc gagtctctgt tggacctccc gctctcgcca
     6001 gatgtcaacg acgatcaggc tgaggtttgt caccgcggcc gatccgacga tggtcgtgat
     6061 cagggccggg agtccggcgg tggcgttgaa tccgagccag gcaccgagga ggccgcttcc
     6121 gacggccacc gcgaaccctc cggtcttgag tcgcgggagc cagtcgcgat gcacccaggc
     6181 ccagtaggtc ccgagaccga tcggcacgcc aatggcgacc acgccgagca gttccgagtc
     6241 gagcgggacg gtcggccaga acgtcagcac gatcagggcg ccgagggacc atccacccag
     6301 gccgagcaca atcgggtaga gcgagcgcaa ggccgcgctc gtcttggggg cgaagctgcc
     6361 ctgtttgcgc acccggcgcg gcatccagag gagcgaggcg accacgacga gcgcgagccc
     6421 gatcatggcg ccggccacga ctttggcgag cgcggtcagg gtgatccgcg gggtgaagtc
     6481 gacggcgatc ggcgtgtaca gcgaggcgtc gacctggccg ctgtcgaaga aagtgttgac
     6541 cagccggctg cccgcctccg gctgctccgc gaagaaactg ccggtgtgac cgagcccggg
     6601 cagcacgatc tgctgaccgt ttggcaggta ggggagcagt tcgttggccg cgacctgcgg
     6661 cggggtcgag gtatccagcg ctccgccgac gaggagcgtc tccacggccg aggtccgcac
     6721 gcgacggtac gtgtcttcgt cggccgtgtt tggccaggca tcggcgagct ggccgccgcc
     6781 ccaggcgaac gccgagccgg cccagccgag gctcgcgcgg tccaggccgg gtgtggcgaa
     6841 atagtcgcgt gccgcctggg cgtcgaggat cccggcggcg gcgtactcgc cccagacgaa
     6901 cggcacgggg aagagcttcg cgaacagcga cacgaaccag aacccgctcg cgtcgcccgc
     6961 ggccgcggat agccaagcat cgaatgcc
//
LOCUS       DASUBI010000671         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_361737, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000671 DASUBI010000000
VERSION     DASUBI010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361737"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="ybaK"
                     /locus_tag="VFU72_12995"
     CDS             complement(<1..691)
                     /gene="ybaK"
                     /locus_tag="VFU72_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEU5424455.1"
                     /translation="MFLAAVAAAGIGSGDVGGQGTRATQRLAALGIAYRVHQYAHDQR
                     ASSYGVEAAAAMGMPAARVFKTLIASVDGQLTVAVVPVSGQLNLKALAEAAGGKKAEM
                     AQTAAAERATGYVTGGISPIAQRKVLPVVIDSSATSFETVFCSAGQRGLEVELAPDDL
                     VLATGATLASIARPGNLAGPRGARRPRAPANLPTAAAGEHCSYPAEGYDSVGPQTLLP
                     VTVPGQRCRSPK"
     gene            complement(772..1902)
                     /locus_tag="VFU72_13000"
     CDS             complement(772..1902)
                     /locus_tag="VFU72_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5424456.1"
                     /translation="MSTAGSTLTRTQQEPIRSRQLHVAMVAPPYFDVPPTAYGGIETV
                     VADLVDSLVARGHEVTLVGAGRHATRAQRFVTTYDVGPADRLGEPMPEIVHAAKVAAI
                     LESLDVDVIHDHTMAGPLMARGRLTPTVVTAHGPVQGDSGDFYRALGDTVQLVAISDA
                     QRSTGPDLPWAATVHNAIRAETFPFSTKKEDYALFLGRFHPEKAPHLAIDAARAAGLP
                     IVLAGKCSEPLERAYFAREIEPRIDRDVTIFGIADAAAKRRLLSRAACMLFPICWEEP
                     FGLVVIEAMVCGTPVVAMRRGAVPELIVNGETGIIVDDLDQMPEGIARARELDPVACR
                     KHVEASFTVEVMAEGYEMVYNQALAVAPAPWMTAVADRAPLV"
BASE COUNT          373 a          766 c          742 g          348 t
ORIGIN      
        1 ccttcgggct acggcagcgt tgacccggaa ccgtcacagg gagcaacgtc tgcgggccga
       61 cgctgtcgta gccctcggcc ggatagctgc agtgttcacc cgctgccgct gtcggaaggt
      121 tggctggggc gcgcggccga cgcgcgcccc gcgggccggc taagtttcct ggcctggcga
      181 tcgaggccaa ggtcgcgccg gtggcgagga cgaggtcgtc gggggccagc tcgacttcca
      241 ggccgcgctg gcctgcgctg cagaacaccg tctcgaagct ggtcgccgac gagtcgatga
      301 cgacggggag caccttgcgc tgggcgatcg gcgagatgcc gccggtgacg tagccggtgg
      361 cgcgctcggc ggcggcggtc tgggccatct cggccttctt gccgccggcg gcctcggcca
      421 gcgccttcag gttgagctgc ccggataccg gcacgacagc cacggtgagc tggccgtcca
      481 cgctggcaat gagagttttg aatacgcgcg cggctggcat gcccattgcg gcggccgcct
      541 ccacaccata cgaactggct cgctggtcat gtgcgtattg atggacgcgg taagcgatgc
      601 caagcgcagc gagacgttgc gtcgcccggg taccttgacc ccccacgtcc ccagatccta
      661 tacctgcggc tgcaaccgca gccaggaaca tagaaccgcg gccaggccga gcagtttcgc
      721 tcagcccggc cgcggccagc agcgcaagcg ccgctaggcg gccgacccgc gctagacgag
      781 cggcgcccgg tcagcgacgg ccgtcatcca gggcgccggt gccacggcca gcgcctggtt
      841 gtagaccatc tcatagccct cggccatgac ctcgaccgtg aagcttgcct cgacatgctt
      901 gcggcaggcg accgggtcga gttcgcgggc gcgggcgatt ccctccggca tctgatccag
      961 gtcatcgacg atgatcccgg tctcgccgtt cacgatcagc tcgggcaccg cgcctcggcg
     1021 catcgcgacg accggggtac cgcagaccat ggcctcgatc acgaccaggc cgaacggctc
     1081 ttcccagcag atcgggaaca gcatgcacgc cgccctggac agcagccgtc gcttcgcggc
     1141 cgcgtcggcg atgccgaaga tcgtgacgtc ccggtcgatc ctcggctcga tctcgcgggc
     1201 gaagtacgcc cgctcgagcg gctcactgca cttgccggcc agaacgatcg gcaggccggc
     1261 ggcccgggcg gcatcgatgg ccaggtgagg cgccttctcc ggatggaacc ggccgaggaa
     1321 gagcgcgtag tcctccttct tcgtgctgaa cgggaacgtc tccgccctga tcgcgttgtg
     1381 caccgtcgct gcccagggca ggtcaggtcc ggtcgaacgc tgggcatcgg agatcgccac
     1441 cagttgcacg gtgtccccga gcgcccggta gaagtccccg ctatcgccct ggaccgggcc
     1501 atgggccgtc accacggtcg gcgtcagccg accacgcgcc atcagcggcc cggccatcgt
     1561 gtggtcgtgg atgacgtcca cgtcgagcga ctcgagaatg gcagccacct tggcggcgtg
     1621 cacgatctcc ggcatcggct ctccgagccg gtcagcaggt ccgacgtcgt aggtcgtgac
     1681 gaaccgctgc gccctggtgg cgtggcggcc tgccccgacc aaggtcactt cgtgtccacg
     1741 cgcaacaagc gagtcgacta ggtcagcgac caccgtctcg atgccgccgt aagccgtcgg
     1801 cggcacatcg aagtagggcg gagcgaccat ggccacgtgc aattgccgcg acctgatcgg
     1861 ctcctgctgg gtccgggtaa gggtcgagcc tgcggtgctc acagcgaacg cctccttgcc
     1921 tcactagatc atcggcgcgg cccgaggctc cggtaagacg cccggttccg gccggaaatg
     1981 atcatgcccg ccgccaggca gcccaatgct gaggtcaatt aataaagcgc taatagctcg
     2041 gtaaagagat tgccttcgct ggaactaatc cttgaagtaa aaacaccggc accaatgttt
     2101 cgccgacgca gctaaagaaa ccagtcgccg ctggcgtgac agcggaactt gataaccggc
     2161 tcgctaccgg cgcatctggc gccacactgg gcgcggaacg acccggagta cattcgcgag
     2221 cacgctgag
//
LOCUS       DASUBI010000672         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_753132, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000672 DASUBI010000000
VERSION     DASUBI010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753132"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..1210
                     /locus_tag="VFU72_13005"
     CDS             458..1210
                     /locus_tag="VFU72_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424457.1"
                     /translation="MPKVDRWARLTVRLPAATYRRLEALTAEIPGLTPNVLLRSAVEQ
                     VLPQLELLAEALRAARAGQGGNALELLAALTQAAGAPPVVPEDEPTAPTPANVEAALA
                     RVRALQVAQAQRLQDVLDQPQGRGARQALERLQTLHGEQAQRVEQAVAATGEDMPALL
                     EWARALQAEQARWLHEILDQPHAEPLQQALAVLAVLQAEQTRSVQRLSDLMLAPVQDD
                     QPVTAAQEQNAAAFHQRWLASTGGTAQRPSST"
     gene            2242..2631
                     /locus_tag="VFU72_13010"
     CDS             2242..2631
                     /locus_tag="VFU72_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424458.1"
                     /translation="MQDAAQVRRAQDQHLVEALPPDAAQAPRADGILPRRAGGRAQIL
                     DNGRYGAAAERRTAVTVAVADEIAWVVAQRGRLAPLLGDPGGGRGRGTPTCTTRRAPS
                     ATTKKAYTGRKSTSVTERQSQAQRAPT"
     gene            2876..3103
                     /locus_tag="VFU72_13015"
     CDS             2876..3103
                     /locus_tag="VFU72_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5424459.1"
                     /translation="MKQTRQLTAIIEREGDGYVALCPELDIASQGDTVEEARDNLAEA
                     LALFFETAAPAEVTSRLHTEVFVTRLDILIG"
     gene            3096..3326
                     /locus_tag="VFU72_13020"
     CDS             3096..3326
                     /locus_tag="VFU72_13020"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU5424460.1"
                     /translation="MGRLRVLSGREVCAILAGHGFAEERRRGSHIVMQRRTNDTTTTV
                     PVPDHRELRAGTLQSIIRQSGLPRALFEVDEG"
     gene            complement(3583..4056)
                     /locus_tag="VFU72_13025"
     CDS             complement(3583..4056)
                     /locus_tag="VFU72_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5424461.1"
                     /translation="MFCTHRGWPLGWRTVDRDFARRMRRAGVRVITFHESRHTCATIA
                     LKHGIPAKVVQERLGHSDIGVTLNIYSHVTPGSASARRRKWARSWCPTLRTRGVRSRP
                     SIRSKSGRNAVRLQATAPSAIFRRGRCLRDSLLPSRNGWSGREDLNLRPPGPQQE"
BASE COUNT          628 a         1425 c         1412 g          599 t
ORIGIN      
        1 cctgccttgc cgccgctcac cacctttctg cccaagccgt tcgacctcga ccacctgctc
       61 gatgtggtcg cgacgctgct cgccacctcc tgacccgtcc ggcgtgcccg aggctcaatt
      121 acctgggtgg ccggcagccg aggcgctcgt cgcgcgtcag cggccacgct gaccacagtg
      181 cctgcggaca cgctggccgg actcgccgga tggcagtggc gtaaccgacc gccatccggg
      241 tgggcactgg tcaggtctgc tggcgcgatg ccgcaagccc cggcggagcc cggaacgcag
      301 ctctctcgca actgtgaact cgtatgggaa cggcaccacg ccccgcacgc ccagtcacca
      361 tctgctatac tggtgacgtc gtatggcgtc acgcatctcc accggtcccc cgttgtcact
      421 agcgcctatt accctacccg aatacgaagg acaaacgatg ccgaaagttg atcgctgggc
      481 gcgcctgacc gtccggctgc cggccgcgac ctaccggcgg ctcgaagccc tgaccgcgga
      541 gatccccggg ctgacgccaa acgtactgct gcggagcgcc gtggaacagg tgctgccgca
      601 actggagctg ctggcggagg ccctgcgggc ggcccgggcc ggtcagggcg gcaacgccct
      661 tgaactgctg gctgcgctga cccaggcggc cggcgcccca ccagtagtcc cggaggacga
      721 gcccaccgcg cccacacccg ccaatgtcga ggcggcactg gcccgcgtgc gggcgctcca
      781 ggtggcgcag gcccagcggc tgcaggacgt cctcgaccag ccgcagggcc ggggtgcgcg
      841 gcaggcgctc gagcggctgc agaccctgca cggtgagcag gcgcagcgcg tcgagcaggc
      901 ggtcgcggcg actggggagg acatgccggc gctgctggag tgggcgcggg cgctgcaggc
      961 ggagcaggca cgctggctgc acgagatcct cgaccagccg catgccgaac cgttgcagca
     1021 ggcgctggcg gtgctggcgg tcttgcaggc cgagcagacg cgttcggtgc agcgactgag
     1081 cgacctgatg ctggcgccgg tccaggatga ccagccggtg accgcggccc aggaacagaa
     1141 tgcggcggcg tttcaccagc gctggctcgc cagcactggc ggtaccgcac agcggccgtc
     1201 gtccacgtag cggcgccagg agcgatcggg ttcgcccctg cgggcgccgg ccaaggccgg
     1261 ccacgcagca ggagccgccg acggctgacg aaccagggct gatccaccga agtcggcacc
     1321 acgacgtctc tgccttgacc acgagccgac tctgaccggc gttgcgtggg tcgctggggc
     1381 accgccagca gcgcagcgtc cggcgcggcg ggtctgagac gcgggactcc ttgttgacga
     1441 tggtggcgct acgcggcggc gatcgcggcg ccatcgtctc aggtcgccgg taggccacgc
     1501 aggacgcagg atggcaggag acgagaccag gacagacgcg cgcggcgaag ctatggccga
     1561 gatgcgcgcg cgagtggctg ggccgaccgt ccctggaggc gccgtgcccg gagatcccgg
     1621 atggaacctc gccgccggct ggcgcggccg ggcgctcgcc tgcagtgccg gcctgatcgc
     1681 ctgtgctggc taccagcctc atccgcagtc gcatcctggc attcgtgacc cgagagtgcc
     1741 aaagcggcca gtggatgagg ctggtagcca gcacaatggt gcaagccacc gaggaccggg
     1801 atcgcgtaga cacgcgcacc gctccgtggg ggcgttcctc gagctggcgc gtcgggcacc
     1861 tggtgacgca gggcttgatg gggtcggtcc tgattgcaat aacccgcgtc ttcccgcagc
     1921 acgcgtcgca ggtgcccttc gctcaagatc agtccatggt ccagggattc acgccgggcg
     1981 ctcccgagga accacgcgca ggtggcagtc tgtcgtggcg cgcgcaaggt cgtccgctgt
     2041 cgtggctggt tggctcatcc gccggccctc gaccaaggct cacgccgcgc gcaggtacgg
     2101 gtgatgcaac ccgcccagca ctggcacggc ccgcaccagc cccctgtcct cgggtcgaag
     2161 tgagcgtacc gctgcggggt cagggacccg ctgccgcagg ccttggtgtg gccgcgcggc
     2221 gttgaagtac gccacatact cgtgcaggac gcggcgcagg tgcgccgcgc ccaggaccag
     2281 cacctggtcg aggccctccc gccggacgct gcccaggcac cgcgcgcaga cggcattctg
     2341 ccgcggcgcg cgggaggccg tgcccagatc ctcgataacg gtcgctacgg cgccgcggct
     2401 gaacgccgga ccgcagttac tgtcgcggtc gcggatgaga tcgcgtgggt ggtggcccaa
     2461 cgcggtcgcc tcgcgccgct gctgggcgac cccggcggcg gtcggggccg cggcacgccg
     2521 acgtgcacca cccggcgcgc gccgagcgcg accacgaaga aggcgtacac cggccggaag
     2581 agcacgtccg tgaccgagag gcagtcgcag gcccagcgcg cgccgacgta gttgcgccgg
     2641 aacgtcgccc acggctgccc cacgcgtcgc ggcgggcggg cggcgcgcag atactgctgc
     2701 acggtccact tggcttgagc tggccatttc gtccatccgg cgcggatcgg ggcgctctcg
     2761 cacccggatg gtcagaagac cgttgcggac cgactcatcg accagttccc tggcagctct
     2821 acgcccaccg gcggcttcgt cgcctggtat actgccgtcg aggagggagg cgcccatgaa
     2881 gcagacccgc cagctcaccg ccatcatcga gcgcgagggc gacggctacg tcgccctgtg
     2941 ccccgaactc gacatcgcca gtcagggcga caccgtcgag gaggcgcgcg acaatctggc
     3001 ggaggccctc gcgctcttct ttgagactgc ggcacccgcc gaggtgacga gtcgcttgca
     3061 caccgaggtc ttcgtgacgc gcctggacat cctcattggg taggttgcgc gtcctctcgg
     3121 gccgcgaggt ctgcgccatc ctggcggggc acggcttcgc ggaggagcgc cgccgcggca
     3181 gccatatcgt catgcagcgg cgcacgaacg acacgaccac cacggtcccc gtgcccgacc
     3241 accgcgagtt gcgcgccggg acgctacagt cgatcatccg ccagtcgggg ctaccgcgcg
     3301 cactcttcga ggtggacgaa ggctgattct cgggaagccc ggcggagttc accgcgagcg
     3361 ttccgccacg cgagatgtcg ttcccacggc tatcatgctg tgtcaccggc ggccctcctc
     3421 gcgcgacacc tggtaacgtc gggagatggt cgcatcgcaa ccgacgctgt gtcgcaaccc
     3481 gatgggctat gcgggcggga ggcgctcctg ctatactgag ccagtcagga ggtttcgctc
     3541 cgggaagcca gatgggttag gggtcaaaac cggcccattt cgttactctt gctgggggcc
     3601 aggtggtcgg aggttcaaat cctctcgccc cgaccaccca tttctcgacg gcagcaatga
     3661 atcacgcaag caacggcccc gtcggaatat tgccgacggg gccgttgctt gcaatctgac
     3721 tgcatttcgg ccggatttac ttctgatgct cgggcggctc cgcacccctc gcgtccgcaa
     3781 cgtcgggcac caggatcgcg cccatttgcg ccgccgcgcg ctggccgagc ccggggtgac
     3841 gtgactgtag atattgagcg tgacgccgat atcgctatgt ccaagtcgct cctgcacaac
     3901 cttcgccggg atgccatgtt tgagcgcgat cgtcgcgcag gtgtgccggg attcgtgaaa
     3961 ggtgatcacg cgcaccccgg cccgccgcat cctgcgcgcg aagtcgcggt ctaccgtgcg
     4021 ccagcctagc ggccagcccc ggtgggtaca gaagatgagg ctgt
//
LOCUS       DASUBI010000673         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_141355, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000673 DASUBI010000000
VERSION     DASUBI010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141355"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFU72_13030"
     CDS             complement(<1..642)
                     /locus_tag="VFU72_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424462.1"
                     /translation="MNSSRWSRRTAVILLLNVVTLMLSACRRGFTQSTPTATPVASGA
                     ATPQADATAIKPQKGGSVVAAIPEQPDSLNFSLASQVTAFWVLSTLDARMVRIRQDGV
                     YEPQLLAEVPTAENGGISEDGRTWTLHFRPKLSWSDGEPLDARDFKFTWATLTDPVYP
                     ALDRTGWASIVGIRLSGDNLTAVLTLRQSDAGLLDQILSGRSDDRRGFLLPAHI"
     gene            732..>3157
                     /locus_tag="VFU72_13035"
     CDS             732..>3157
                     /locus_tag="VFU72_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424463.1"
                     /translation="MLQRQRQRAQLALRSHAAYHARIRIAPKGSNVAGASREPYLFVS
                     YASADRERVLPVVDRLQHAGVNAWIDREGIHGGANYAQVISDAIKGAAALMLMASPAS
                     LASRNVRQELALGWRFERPYLPLLLDRITIPDELAYWLEGSQWIELLDHPEDVWLAGM
                     AQALGHLGIEIRLGAGPVERATPPRERPPLVGREHEQALLSQQLARMLGGRGGVVLIG
                     GEAGIGKSTLVEDLSAQAEESGALVLWGHAYDLSVTPPYGPWVEILRQYPASAPGMPL
                     PPTFVGDAEALAKIGSQETLFAATRDFFQAVAAQRPLVLVLNDLHWADQSTLDFFRFL
                     ARQLANQRILLVATYRTDEVNRRHPLHILLPLLVREAGAERLVVRPLDAAGHRALIQS
                     RYALAEADQVRLEDYLEARAEGNPLYAGELLRTLEEERILTRQDDDWVLGDLEQVRVP
                     ALLRQVIEGRAARLDEESQRLLGIAAVIGQTAPLVIWAAAGEVDEDSLLELAERAEEA
                     GLLEALPDGDGVRFSHALIRSTLYEVLPPLRRRRLHRQVADVLLALPGPDPDSVAFHL
                     EQGGDARAIDWLIRAGERALRLYAFDAAIAHFSQAESLAARQTAAVPGTLYASRGQAY
                     RLTGQFDAARHDFETVLTLARQSGDPRVEWQADIDLGMLWAARNYARTGEYFQQALAL
                     ARTMDAPDLTAHALNRVGNWHVNSQQPLNALRHHEEALKIFEQLGDRAGIAITSDLLG
                     LASYLAGDLIQGTMHYRRAVELFREADNREGLSSALAVMSMRGGTFMTDTMVVEQVTL
                     DECVRDAEEAV"
BASE COUNT          516 a         1015 c         1095 g          531 t
ORIGIN      
        1 gatgtgcgcc ggcaggagga aaccgcgtcg atcatcgctt cgaccggaga ggatctggtc
       61 gagcaggccg gcgtcggatt ggcgcagggt caggaccgcg gtcaggttgt cgccgctcag
      121 gcggatgccc acgatcgatg cccaaccggt tcggtcgagc gcagggtaga cggggtcggt
      181 caaggtggcc caggtgaact tgaagtcgcg cgcgtcgagc ggctctccgt cggaccaact
      241 gagcttgggt cggaagtgga gcgtccaggt gcggccatct tccgagatgc cgccgttctc
      301 ggccgtgggc acctcggcca gcaattgcgg ctcgtagacg ccgtcttgcc ggatgcggac
      361 catgcgcgcg tccagcgtgc tgagcaccca aaaagccgtg acctgactcg ccagcgaaaa
      421 gttgagtgag tcaggctgct ccgggatcgc ggcgaccacc gaaccgccct tctgcggctt
      481 gatcgcggtc gcatcggcct gtggcgtggc ggcaccactg gcaactgggg ttgccgttgg
      541 cgtggactgg gtgaagccgc ggcgacaggc ggagagcatc agcgtgacaa cattcaggag
      601 aaggatcacc gccgttcgcc gcgaccatcg cgacgaattc actggaactc cttgggccag
      661 catgagtcga cgcgggtgcg cggcgacctg ctcctccggc gcgacgacgg catgacacgg
      721 cgattatagt gatgctgcag cgccagcgtc aacgcgcgca gcttgcgttg cggtcccacg
      781 ccgcgtacca tgcgcgtata cgcatcgcac cgaagggcag caacgtggcc ggagcgtcca
      841 gggaacccta cctgttcgtc agctatgcca gcgccgatcg cgagcgcgtg ctgccggttg
      901 tcgaccgtct gcaacacgcg ggcgtcaacg cgtggattga ccgtgaagga atccacggcg
      961 gagccaacta cgcccaggtt atcagtgacg ccatcaaggg agctgctgcc ctgatgctga
     1021 tggcctcacc cgcctcgctg gcctcccgca acgtcaggca ggaactcgcg ctcggctggc
     1081 gcttcgagcg gccatatctg ccactcctgc ttgaccgcat cacaatcccg gatgagctgg
     1141 cctactggct ggaaggctcg cagtggatcg agttgctcga tcaccccgag gacgtctggc
     1201 tggctggcat ggcgcaggcg cttgggcatc tcggcatcga gatccgcctg ggcgccgggc
     1261 cggtagagcg tgcgacgccg ccgcgggagc ggccaccgct ggtcggccgg gagcacgagc
     1321 aagcactcct cagccaacaa ctcgcccgca tgctgggcgg tcgcgggggc gtggtgctca
     1381 tcggcggaga agcggggatt ggcaagtcca cactggtgga ggacctgagc gcgcaagcag
     1441 aggaatccgg cgccctcgtc ctctgggggc atgcctacga cctgagcgtt acgccgccct
     1501 atggtccctg ggtggagatt ctgcgccagt atccggcgtc cgcgccaggc atgccgctgc
     1561 ccccgacctt tgtcggcgat gccgaggcac tcgccaagat cggctcccag gagacgctct
     1621 ttgccgcgac cagggatttc ttccaggcgg tggcggcaca gcgccccctg gtgctggtgc
     1681 tgaacgatct gcactgggcc gaccagtcca ccctcgattt cttccgcttt ctcgcccggc
     1741 agctcgcgaa ccagcgcatt ctgctggttg ccacctaccg taccgatgag gtgaaccggc
     1801 gacatcccct tcatattctg ctgccgctcc tggtgcggga ggccggggcc gagcgattgg
     1861 tcgtgcggcc gctggacgcg gcggggcacc gtgcgctgat tcagagtcgg tacgccttgg
     1921 cggaggcgga ccaggtgcgg cttgaggact atctggaagc gcgagccgag ggcaatccgc
     1981 tctacgccgg cgagctcctg cgcaccctgg aggaagaacg catcctgacg cggcaggatg
     2041 acgattgggt gttgggcgac ttggaacagg tgcgggtgcc agcgctcttg cgccaggtaa
     2101 tcgaggggcg cgcggcgcga ttggacgagg agagccagcg tctcctgggg atcgcagcgg
     2161 tcatcgggca aacggccccg ctggtgattt gggccgcggc gggggaggtc gacgaggata
     2221 gcttgctcga actcgcggag cgcgcggagg aggccgggct gctggaggcg ctgccggacg
     2281 gtgacggcgt gcggtttagt cacgcgctca tccgcagcac actctacgag gttcttccgc
     2341 cgctgcggcg ccggcggctg caccggcagg tcgccgacgt gctgttggct ctgcccggcc
     2401 cggaccccga ctcggtggcc tttcacctgg agcagggcgg cgacgcccgg gccatcgact
     2461 ggctgatccg ggccggggaa cgagcgctgc gcctctatgc cttcgatgcc gccatcgcgc
     2521 atttcagtca ggccgaatcc ttggcggcgc ggcaaaccgc cgcggtaccc ggcacgctgt
     2581 acgcctcacg cggccaggca taccggctta ccggccaatt cgacgccgcc cgccacgatt
     2641 ttgagaccgt gctgacgctc gcgcgccagt ccggcgaccc gcgcgtcgag tggcaggcag
     2701 atatcgatct cggcatgctc tgggcagctc ggaactacgc gcgcaccggc gagtatttcc
     2761 agcaggcgct ggcgctcgcc cggactatgg atgcgccgga cctcacggcg cacgcgctca
     2821 accgggtcgg caactggcac gtcaatagcc agcaaccgtt gaacgcactg cgtcaccatg
     2881 aggaggcgct caagatcttc gagcagctcg gtgatcgcgc cggaatcgcc atcacctccg
     2941 atcttctcgg tctggccagt taccttgccg gagatctgat tcaggggacg atgcattatc
     3001 ggcgtgcagt cgaactcttc cgggaggccg acaatcgcga agggctgtcc tccgctctgg
     3061 cggtgatgtc gatgcgcggc ggaaccttca tgaccgacac catggttgtg gaacaggtta
     3121 cgctggatga gtgtgtgcga gacgccgaag aagcggt
//
LOCUS       DASUBI010000674         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1353933, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000674 DASUBI010000000
VERSION     DASUBI010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353933"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="VFU72_13040"
     CDS             complement(<1..1696)
                     /locus_tag="VFU72_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424464.1"
                     /translation="MSDQENGEQPMMMPQRLVGGRFSRRAVLVRGALAGAGATLSALL
                     AACGGSSKSPATQVAGTASSGSATATAAGGTSATAPAASLATPKTVPATPVAVATEAA
                     GQPGGTLNFGLLRDPIALDPHIAYGASSASLQGNLYDTLVTYDRAANIVPSLAKSWQI
                     QDDGKSYVFTLQEGVTYHDGAPLKAEDVVFTFDRIRDKNTGANLFSELSNLDSYTATD
                     DKTVTITLKQPYAVLMSVLASQWCYIVNKAWAQAGNDFTKKENGTGPFTLQGFEPQVK
                     YTLAKNPKYWVKGRPYLDQIVETIIADDTARMNAFKSGQMNFVEYVPWQNMDELGANS
                     QYKLYIGFDTFNLVRLNPQKPPLDNKLVRQALNYAIDRKAVVDVAFGGKGQAMTAGLI
                     PKGTFWYNDELDGHWTYDTKKATDLLQQAGFKPSDIKLDFAVANISVHSDTAQAVAQQ
                     LKQLGMSINIIQQDVPTLTKRRTTGEYQMMQDGLSEPWTDPDMYTTFFGTGGASYAKG
                     VNFSDPKLDDLLAQGRQETDNNKRKAIYKQFEQQLFDDAPWIFILWRPQAEVSTANL"
     gene            complement(1753..2439)
                     /locus_tag="VFU72_13045"
     CDS             complement(1753..2439)
                     /locus_tag="VFU72_13045"
                     /inference="COORDINATES: protein motif:HMM:NF004850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424465.1"
                     /translation="MSLKPGFKIVKSFRRPDPELIANFRGLAAANISDVQGRQNTLDA
                     RVKPIYHPMPPLCGPALTVKARTGDNLMAFKAIELARPGDVIVISGGLDLNYSLWGGV
                     MSTMAAQNGIAGVVTDGLVRDVAQTRNVGLPVYAIGLTPTGPTKEGVGQINTPIACGG
                     VVVNPGDIVVGDEDGVVVVRREEAETVLERVHARIALEQSWFARIAQGELFLMDSDEE
                     LRARGAEIVD"
     gene            complement(2436..>2630)
                     /locus_tag="VFU72_13050"
     CDS             complement(2436..>2630)
                     /locus_tag="VFU72_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424466.1"
                     /translation="ERVKDHTPAGGARYPDILQYQGMKARSAVGCTGNPEAATPEKGE
                     ELVKRGVERIVAFVREEFGG"
BASE COUNT          370 a          860 c          896 g          504 t
ORIGIN      
        1 gcaggttggc ggtgctgacc tcggcttgcg gacgccagag gatgaagatc cacggcgcgt
       61 cgtcgaagag ctgctgctcg aactgcttgt agatcgcctt gcgcttgttg ttgtcggtct
      121 cctggcgccc ctgagccagc agatcgtcga gcttggggtc gctgaagttg acgcccttgg
      181 cgtagctggc gccgccggtg ccgaagaagg tggtgtacat gtccgggtcg gtccacggct
      241 cgctgaggcc atcctgcatc atctggtact cgccggtggt gcgtcgcttg gtcagcgtcg
      301 gcacgtcctg ctggatgatg ttgatcgaca tgccgagttg cttgagctgc tgggccacgg
      361 cctgggccgt gtcgctgtgc accgagatgt tggcgaccgc gaagtcgagc ttgatgtcgg
      421 acggcttgaa ccccgcctgc tgcagcaggt cggtcgcctt cttggtgtca taggtccagt
      481 gcccgtcgag ctcgtcgttg taccagaagg tgcccttggg gatcagcccg gcggtcatcg
      541 cctgcccttt gccgccgaag gcgacatcga cgaccgcttt gcggtcgatg gcgtagttca
      601 gcgcctggcg caccagcttg ttgtcgagcg gcggcttctg cgggttgagg cgcaccaggt
      661 tgaaggtatc gaaaccgatg tagagcttgt actgggagtt cgcgcccaac tcgtccatgt
      721 tctgccaggg tacgtactcg acgaagttca tctggccgct cttgaacgcg ttcatgcgcg
      781 cggtgtcgtc ggcgatgatc gtctcgacga tctgatcgag gtacggccgc cccttcaccc
      841 agtacttggg attcttggcc agcgtgtact tcacctgtgg ctcgaacccc tgcagcgtga
      901 acgggccggt gccgttctcc ttcttggtga agtcgttgcc ggcctgcgcc caggccttat
      961 tcacgatgta gcaccactgg ctggccagca ccgacatcag cacggcgtag ggctgcttca
     1021 gcgtgatcgt gaccgtctta tcgtcggtgg cggtgtagct atcgaggttg ctgagctcac
     1081 tgaagagatt ggcgccggtg ttcttgtcgc gaatgcgatc gaaggtaaaa acgacgtcct
     1141 cggccttgag cggggcgccg tcgtggtagg tcacgccctc ctgcaaggtg aagacgtagg
     1201 acttgccgtc gtcctggatc tgccacgact tggccagcga cggcacgatg ttggcggccc
     1261 tgtcgtaggt gacgagcgtg tcgtagaggt tgccctgcag cgaggccgac gaggcgccgt
     1321 aggcgatgtg cgggtcgagc gcgatcgggt cgcgcagcag cccgaaattg agggttccgc
     1381 ctggctgacc ggccgcctcc gtggccacgg ccaccggagt cgccggcacg gtcttggggg
     1441 tcgccaggct ggccgcgggg gcggtggccg aggtgccgcc cgcggctgtc gcggtggcgg
     1501 agcccgagct ggcggtgccg gccacctgtg tggctggtga cttcgagctg ccgccacacg
     1561 ccgccagcaa cgcgctcagc gtggcgccgg cgcccgccag cgcgccgcgc accagcacgg
     1621 cgcggcgcga gaaccgcccg cccaccaggc gctggggcat catcatcggc tgctcgccgt
     1681 tctcctgatc actcatgatt cacccctcct catatcccgc cggtgaccaa cgatgccgcg
     1741 aagtgtaccg ggtcagtcca cgatctcggc gccgcgggcg cgcagctctt cgtcggagtc
     1801 cattaggaag agttcgccct gggcgatgcg cgcgaaccac gactgctcca gcgcgatgcg
     1861 ggcgtggacg cgctccagga ccgtctcggc ctcctcccgg cgcaccacga ccacgccgtc
     1921 ctcatcgccg accacgatgt cgccgggatt gaccaccacc ccgccacagg cgatcggggt
     1981 gttgatctgg ccgacgccct ccttggtcgg gccggtcggc gtcaggccga tggcgtagac
     2041 cggcaagccg acgttgcgcg tctgggcgac gtcgcgcacc agcccgtcgg tgacgacccc
     2101 ggcgatgccg ttctgcgccg ccatcgtgct catcaccccg ccccagagcg agtagttcag
     2161 gtcgagcccg ccgctgatca cgatcacgtc gccgggccgg gccagctcga tcgccttgaa
     2221 cgccatcagg ttgtcgccgg tgcgcgcttt gaccgtcagg gccgggccac agagcggtgg
     2281 catcgggtgg tagatcggct tgacacgggc gtcgagcgtg ttctgacggc cctgcacgtc
     2341 ggagatattc gccgccgcca ggccgcgaaa gttcgcgatc agctccgggt ccggccggcg
     2401 gaacgatttg acgatcttga agcccggttt gaggctcatc cgccgaactc ctctcgcacg
     2461 aaggccacga tccgctcgac gccgcgcttg acgagctcct cccccttctc gggggtggcc
     2521 gcctctgggt tgccggtgca gccgacggcc gagcgcgcct tcatgccctg gtattgcagg
     2581 atgtccgggt accgggcacc cccggcgggg gtgtggtcct tgacccgctc
//
LOCUS       DASUBI010000675         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_87006, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000675 DASUBI010000000
VERSION     DASUBI010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87006"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            820..1434
                     /locus_tag="VFU72_13055"
     CDS             820..1434
                     /locus_tag="VFU72_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU5424467.1"
                     /translation="MLIPALGLGLAYAATPGVVNTECVRRGLARGFRPAFLVQLGALL
                     GDGAWAVVALAGVATLAQDRSLGVALGVVGGLFLCRLAFAALREAWRGPRLAPTQVTQ
                     RGDFGTGAVFGLANPAGLAFWAGVGGGMLATTSEVGVNGYAAFLAAFLAGALGWSLGL
                     AAFVGWGRRYVRPSLFRAIDALCGTVLGWFGIRLLWGALRHVVA"
     gene            1634..>2923
                     /locus_tag="VFU72_13060"
     CDS             1634..>2923
                     /locus_tag="VFU72_13060"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5424468.1"
                     /translation="MNVTSQAIGSRQASVGMTPPTDDVMALGGVAASVHPFVTETALD
                     VIRQGGNAVDAAVAAAAVLTVVEPRNGHLGGDTFMLINLPGENRVVALNGSGAAPAAA
                     SLEQYVARGGIPKDGLWAASVPGTVHCWGTAVERYGSMPLAGLLAPAIGYARAGVPVS
                     RRLRRMLANEAERFRRFPASARIFVPDGRVPEVGEIFRQPELARSLERIARDGWQDFY
                     SGALARELVQASDELDGLFSLDDFATHDTLERAPLSSDYRGYTVFEQPPVSQGIIVLL
                     ALNILRQFDLPSLGPATAATIHLQIEALKLAFEDALCHLGDPDHVELPLEALLSDEHA
                     RKQAARIRPEAAMPFPWPGNIQPDTTYMCAADGQGTVVSYIHSLFAGCAVVLGDTGAL
                     MNNRMLGFNLTPGHPNCLAPGKRPIHTLNSYLVQRDGE"
BASE COUNT          397 a         1008 c         1012 g          506 t
ORIGIN      
        1 aagcggatca cgtgttgctg gctcatcgtc cccctccttg ccctcgccaa cgccgacggt
       61 gcaccagtct accgcccggg gccgaaccgg acaaatgacg acggccggac cactgagctt
      121 gtcgaaggat ccgtcctcac acgccgccaa tgcccctcgt ccgccgccct ccgctcgtgg
      181 gcgctctgtg tcggtcgggc gtggccatcg actctgctgg gtgccgagtc agcacggatc
      241 cttcgacaag ctcgggatga cagggtgaga gtcgggctga cgaggaggat gggtcttggg
      301 cgacgacctt catcgtccgg taggccccct actacctctg tcatcctgag cttgtcgaag
      361 gatccgtctt ggagcggcac agtgctcgtc ggaagggggg cgaacgcgag ggcacggaga
      421 tcttcctgtg aaacgaggca gaaaaacgcg atcccgacga gcctctcgtc atcgacgccc
      481 tgttcgtttc gatgaggaac gaccgcgggt tgccccggct gccgtcgact cttcgccgcg
      541 aactgctttg acgaccgttg ccggtcctcg ccgctggctg ctccagtttc ccggagccgc
      601 aaccagatca gtcgcctgat ccggtttggc gtcgatccgt ggatcactcc tcgggaccgc
      661 acgtacacaa tagcagccct ctgcctggct gtcaataccc ttcgaagcgg atttgccggt
      721 tcgcttgagg ctcgatctgc cggccacgcc gacgccgata ttgctacact ccccccgcaa
      781 ggtggtggag gtggaggtgg aggtgggcgc ttggtctcgg tgctgattcc cgcgctcgga
      841 cttgggctgg cctacgcggc gacgccgggc gtcgtcaaca ctgagtgcgt ccgccgcggc
      901 ctggcgcggg ggttccgtcc cgcgttcctc gtccagctcg gcgcgctgct cggcgatggt
      961 gcctgggcgg tcgtcgcgct ggccggggtg gcgacgctgg cccaagaccg ctcgctcggc
     1021 gtcgctctcg gcgtcgtcgg cggcctgttc ctctgccggc tggcgttcgc ggcgctgcgc
     1081 gaggcgtggc gtggtcctcg tttggccccc acccaggtaa cgcagcgggg cgacttcggc
     1141 accggggcgg tcttcggtct ggccaatccg gccgggttgg ccttttgggc cggcgtcggc
     1201 ggggggatgc tcgccaccac atccgaggta ggcgtcaacg gctacgccgc cttcctggcc
     1261 gcgtttctgg ccggggcgct cggctggtcg ttggggttgg ccgccttcgt cggttgggga
     1321 cggcgctacg tccgcccctc actcttccgc gccatcgatg ccctctgcgg caccgtgctt
     1381 ggctggttcg gcatccggct cttgtggggc gcgctgcgcc acgtcgtggc ctagcagctg
     1441 aaatcgccgc gccggcatcg ttcgcgcccg ctgattgcag cccgtccgcg attgtcactc
     1501 aatggccgca atccctaaat caaaaagccg aacaccgccg tgttctcatc ggcgtgcgac
     1561 aatgtccgag gctactggcg ctggagaaat acgcggcggg gtgggcatcc tcgaagaggc
     1621 acggagggca gcgatgaacg tcacgtcaca ggcaatcggt tcgcggcagg cgagcgtggg
     1681 catgacgccg cccacggatg acgtgatggc gctggggggc gtggccgcct cggtccaccc
     1741 cttcgtgacc gagacggcgc tggacgtgat ccgccagggc ggcaacgccg tcgacgcggc
     1801 ggtcgcggcg gcggcagtcc tcacggtcgt cgagccgcgc aacggccacc tgggcggcga
     1861 caccttcatg ctgatcaatc tccccggcga gaaccgggtg gtggcgctca acggcagcgg
     1921 cgcggcgccg gccgccgcct cgctggagca atacgtggcg cgcggcggca tccccaagga
     1981 tggtctctgg gcggcgtcgg tgcccggcac ggtccactgc tggggcacgg cggtcgagcg
     2041 ctacggcagc atgccgctgg ctggcctgct ggctccggcg atcggctacg cgcgcgctgg
     2101 cgtgccggtg tcgcgccgcc tgcggcgcat gctggcgaac gaggccgagc gcttccgccg
     2161 cttcccggcc agcgcccgca tcttcgtgcc ggacggccgc gtgccggagg tgggcgagat
     2221 cttccgccag cccgagctgg cgcgctcgct ggagcggatc gctcgcgacg gctggcagga
     2281 tttctattcc ggtgcgctgg cgcgcgagtt ggtgcaggcg tcggatgagc tggacggtct
     2341 cttcagcctg gacgacttcg ccacgcacga cacgctggag cgggcgccgc tcagcagcga
     2401 ctatcgcggc tacaccgtct tcgagcagcc gccggtgtcg caggggatca tcgtgctgct
     2461 ggcgctcaac atcctgcgcc agttcgacct gccgtcgctc ggtccggcca cggccgcgac
     2521 gatccacctg cagatcgaag cgctgaagct ggccttcgag gatgcgctgt gccacctggg
     2581 cgaccccgat cacgtcgagc tgccgctcga agcgctcctc tcggacgagc acgcgcgcaa
     2641 gcaggcggcg cgtatccggc ccgaggctgc catgccgttc ccctggcccg gcaatatcca
     2701 gcccgacacg acctacatgt gcgcggccga tgggcaaggc acggtcgtct cctacatcca
     2761 cagcctgttc gccggctgcg ccgtggtgct gggagacacc ggggccctga tgaacaaccg
     2821 catgctcggc ttcaacctga cgccggggca ccccaactgc ctggcgccgg gcaagcggcc
     2881 gatccacacg ctgaatagct atctggtgca gcgcgacggc gag
//
LOCUS       DASUBI010000676         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_618452, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000676 DASUBI010000000
VERSION     DASUBI010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618452"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFU72_13065"
     CDS             <1..828
                     /locus_tag="VFU72_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424469.1"
                     /translation="VIQGIGVYFSSGDSGDEIANVGLRTVDWPAASPWVTAVGGTSLA
                     VGASNNYLFETGWGTTRSTLADCQSQSQSQTKGKGKSSSSTSTSQCWTPTPPGNYLYG
                     SGGGTSRIFAQPWYQVGVVPSAIANYFGPAGRAVPDVAMVGDPNTGMLVGQTQTFADG
                     TYYDTYRIGGTSLSSPLFAGLMALADQKAGHPHGFANPALYGAAGTSAFRDVVDPAST
                     VAVVRVDYVNGQNGDDGLRTSLRTMNQTGTLHTRPGYDDVTGVGTPNGSNFLDALGQ"
     gene            complement(889..1215)
                     /locus_tag="VFU72_13070"
     CDS             complement(889..1215)
                     /locus_tag="VFU72_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5424470.1"
                     /translation="MFVVVARYLTREGQDGRVAALLREMAAYSNSDAEPRCALYIVNR
                     GIEEPRRFLLYEQYVDRDAFASHTGSDPFQRIILDQVVPLLEERERDFFEVIAAPGLP
                     GLQAPP"
     gene            1357..1617
                     /locus_tag="VFU72_13075"
     CDS             1357..1617
                     /locus_tag="VFU72_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424471.1"
                     /translation="MRTRKEIEALALELEDVIGLADPMTHPEVYDEMQDDPDFAYLSA
                     ISDTLDWVLDEIPTDEFLSEYLDLDALRKRIQAAAKQHPAGS"
     gene            complement(1614..>2013)
                     /locus_tag="VFU72_13080"
     CDS             complement(1614..>2013)
                     /locus_tag="VFU72_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5424472.1"
                     /translation="LVSALGWVVVSTVPRPLVARYGPALVAGWMIVCAAVVTLPLGLS
                     TLGELIANPPSLLAWGALIYSSVLGMFMANVLWQRAVHHLGSTQTMAYLYLQPFLALL
                     LSAALLGERLTPTQALGGLIALAGVGLVRR"
BASE COUNT          357 a          720 c          682 g          254 t
ORIGIN      
        1 gtgatccagg gcatcggcgt ctacttctcc tcgggcgaca gcggcgacga gatcgccaac
       61 gtcggcctgc gcacggtcga ctggcccgcc gccagcccgt gggtgacggc ggtcggaggt
      121 accagcctgg ccgtcggggc cagcaacaac tacctcttcg agacaggctg gggcaccacc
      181 cgcagcacgc tcgccgactg ccagagtcag agccagagcc agacgaaagg caagggcaag
      241 agcagcagca gcaccagcac cagccagtgc tggacgccga cgccgcccgg caactatctc
      301 tacggctcgg gcggcggcac cagccgcatc ttcgcccagc cctggtacca ggtgggcgtc
      361 gtgcccagcg cgatcgccaa ctacttcggc cccgcgggac gggcggtgcc ggacgtggcg
      421 atggtcggtg accccaacac cggcatgctg gtcggccaga cacagacctt cgccgacggc
      481 acctactacg acacctaccg catcggcggc accagcctct cctcaccgct cttcgccggc
      541 ctgatggcgc tggccgatca aaaggcgggg cacccgcacg gcttcgccaa cccggcgctc
      601 tacggcgccg ctggcacgtc ggccttccgc gatgtagtcg atccggcatc cacggtggcc
      661 gtggtgcgcg tcgactacgt caacggacag aacggggatg acgggctgcg cacgtcgctg
      721 cgcaccatga accagaccgg gacgctgcac acccggcccg gctacgacga cgtgaccggc
      781 gtcggcacgc cgaacggcag caatttcctc gacgcgctgg gccagtaagc gcacggctca
      841 ggcggcaagc acgggtggcc gggattcccc ggccgcccgt ccgcctcgct acggcggcgc
      901 ctgcaagccg ggcaaacccg gcgcggcgat cacctcgaag aaatcgcgct cgcgctcttc
      961 gagcagcggc acgacctgat ccaggatgat gcgctggaag ggatcgctgc cggtgtggct
     1021 ggcgaaggca tcccggtcga cgtactgctc gtagagcagg aagcggcgcg gctcctcgat
     1081 gccgcgattg acgatgtaga gcgcgcagcg cggctcggcg tcggaattag agtaggcggc
     1141 catctcgcgc aagagcgcgg ccactcgccc gtcctgtccc tcccgcgtga gatagcgcgc
     1201 gacgaccacg aacatggagc ctcctctcgc tggccggtgg cggtcatccg gcaccgttca
     1261 gccgccgcac gacccgggcc ggcgcgtgcg ggcttgcggc acttcttttg cgcgccgcca
     1321 gcagcgatga tggacgacca gggaaagggg gaccgcatgc gcacccgcaa ggagatcgag
     1381 gcgctggcgc tcgagctgga ggacgtgatc gggctggctg acccgatgac ccaccccgag
     1441 gtctacgacg agatgcagga cgaccctgac ttcgcctatc tcagcgcgat ctccgacacc
     1501 ctcgattggg tgctggacga gatcccaacc gacgagtttc tctccgagta cctcgacctc
     1561 gacgcgctgc gcaagcgcat tcaggccgcg gcgaagcagc acccggccgg cagctagcgt
     1621 cgtaccagcc cgacgccggc cagggcgatc agcccgccca gcgcctgcgt cggcgtcagc
     1681 cgctcgccga gcagcgccgc cgagaggagc agcgccagga acggttgcag gtagaggtag
     1741 gccatggtct gcgtcgagcc gaggtggtgg accgcccgct gccagagcac gttggccatg
     1801 aacatgccca gcaccgagct gtagatcagc gcgccccagg ccagcaacga cggcgggttg
     1861 gcgatcaatt cgcccagggt gctcagcccc agcggcaggg tgacgaccgc cgcgcagacg
     1921 atcatccaac cggcgacaag cgcggggccg tagcgcgcga cgagcgggcg gggcacggtc
     1981 gagacgacca cccagcccag cgccgagacc agc
//
LOCUS       DASUBI010000677         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_41948, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000677 DASUBI010000000
VERSION     DASUBI010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41948"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFU72_13085"
     CDS             <1..342
                     /locus_tag="VFU72_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5424473.1"
                     /translation="YWLLLQLWKHGPLSIGELADAVGVSQSAATTACKRLERAGLVTR
                     ERQASDERVVLVELTGQGREQFEAWRRHRRDVLAHLLEPLGEDERVELQRLIEQVLAR
                     AAEESYVGARS"
     gene            552..1163
                     /gene="rpsD"
                     /locus_tag="VFU72_13090"
     CDS             552..1163
                     /gene="rpsD"
                     /locus_tag="VFU72_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5424474.1"
                     /translation="MARYTGPKHKISRREGVNLTGTRSRSLERRLRVPPGQHSHARPR
                     RPTEYGRQLREKQKLKRHFGMLEKQFRRVFAEADRSQAPTGQELLRLLEQRLDSVVYR
                     LGFAPTRPMARQLVNHGHVLVNGRRVDIASFRVPQGATIALKPIAAEIPAVIEALTET
                     SGRVPSWLSRNGNEPEGRVVGPPVVEEFDMPVDVELVIAFYTR"
     gene            1504..2091
                     /locus_tag="VFU72_13095"
     CDS             1504..2091
                     /locus_tag="VFU72_13095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5424475.1"
                     /translation="MGFAASVPEPGGYSVIGRQVALGPLRRELAPLYEAWRGNFTTLR
                     PLFELGESDELEEPEGWYEEMTVSDGHVWFTIYELAGGKPIGLVGLIDIDFEEKAAEF
                     FLHVGEVASQAADLGSEAGRLMRDYAFHALGLSSLFSMAYEYNLSELRYCETAGFREF
                     TRQRQAHLVGGRLWDVIYLECLAGGPAATGPASSC"
     gene            complement(2106..3359)
                     /locus_tag="VFU72_13100"
     CDS             complement(2106..3359)
                     /locus_tag="VFU72_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5424476.1"
                     /translation="MLPFRARGARGLWPGGQRVVALFLALVLIVAVGAACTLSGSSAS
                     SGSTATGTTAGSATSTSKPLVLASDASSATKTTTSNAPSQADFNNSVIQVAKTAKPAV
                     VEITNEQTSVSQLAQPIPVGTGSGFIYDNQGHILTNNHVVEGAQSLVVTLPDGRSFKD
                     NDVKVVGTDPLTDLAVLQISGSNLPAVQLGDSSQLQVGQWVVALGYALGLEGEPTVTA
                     GVVSALNRSVQEPGNSSGPFSRQSQAGPTLSDVIQTDAAINPGNSGGPLTNLDGQVIG
                     INTLVAGEAEPGVQAEGIGFAIAINTAKQIADEIVSTGKATHPYVGVSYIPLTPAIAA
                     QLNVTATKGALVQSVVDGSPAAKAGLQKGDIVTKVDGQELTTDSSFANTVESHKVGDT
                     LTLTVVRNGQTMQVPVTLAERPAGS"
     gene            complement(3711..4358)
                     /locus_tag="VFU72_13105"
     CDS             complement(3711..4358)
                     /locus_tag="VFU72_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424477.1"
                     /translation="MSGSRLRWAIFPASVVSAVDNPDAYLLRALGQVLAERGDQATFF
                     EERANPAVRALLRRSGGQALADFRARHPEIDYRTTDPRTGADLVEWLTRTLGTYDIAL
                     ALAAAPAELVGWLGRLSRTHLRTYLLDTGYEPALDEAAIEAREPGNFTGIFAGSDTIA
                     ARYGRFIALARIHHLGPLPEPDDQALDRLRAVAARLVETVALAPDARAADHDAGG"
     gene            complement(4405..5010)
                     /locus_tag="VFU72_13110"
     CDS             complement(4405..5010)
                     /locus_tag="VFU72_13110"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5424478.1"
                     /translation="MNARKARVEIDWKKRAGEAASRAPRRQRRQRHPLRWGLLGAATG
                     AAAMFLLDPRTGNRRRALVRDQFVHFGNVAGDFASDTLPQKLDYASGFVRGLQHQAME
                     TMGGGQSHWTDDSQTITDRVMSEVFRDPDLPKGEINVNTVDHVVYLRGHVDNPTLVKE
                     IEERTRKVPGVRNVVNLINHPEVDPSAVRPGFENRTENPQR"
     gene            complement(5118..>5524)
                     /locus_tag="VFU72_13115"
     CDS             complement(5118..>5524)
                     /locus_tag="VFU72_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5424479.1"
                     /translation="LRRYGADDAFARLFNDLLDAVPHFNYLRLPELFCGFQRDAERVS
                     IPVAYPVSCSPQAWASGTIPFLLTALLGVEPDATRRQLRLRPHLPAWLPEVRLDGLRF
                     AGQTVDLVLRGNGADVELSAQASGDVDVCLLG"
BASE COUNT          833 a         1860 c         1945 g          886 t
ORIGIN      
        1 tactggctcc tcttgcagct ctggaagcac gggccgctga gcatcggcga gctggccgac
       61 gcggtcggcg tgtcgcagag cgcggccacg accgcctgca agcggctgga gcgcgccggg
      121 ctggtaacgc gcgagcgcca ggcgagcgac gagcgggtgg tgctggtgga gctgaccggg
      181 caagggcggg agcagttcga agcctggcgc cgccaccggc gcgacgtgct ggcccatctg
      241 ctggagccgc tcggcgagga cgagcgcgtc gagctgcagc ggctgatcga gcaggtgctg
      301 gccagagccg ccgaagagag ctacgtcgga gcgcggagtt agtaccttcc cctatccgac
      361 ctgcttgagg tccgattgtc gtagttgggc ggacaacctg gcgcgaaatg gtgtaagctt
      421 gggcgatgcg ccctacgaga gtttggcggc tgcgctctcc acggcgcgtt tcagcgtgca
      481 tgatcagacc gtgagtggaa caggccaagg tacagggtac actgggtaca gagaggaacg
      541 cgcgaacatc tatggcccgc tataccggac cgaagcacaa gatctcgagg cgtgagggcg
      601 tcaatctgac cggcacacgg tcacgctcgc tggagcggcg gctgcgcgtg ccaccgggac
      661 agcactcgca cgcccgcccg cgccggccaa ccgagtacgg gcgccagttg cgcgagaagc
      721 agaagctgaa gcgccatttc ggcatgctgg agaagcagtt ccggcgcgtc ttcgccgaag
      781 ccgatcgctc ccaggcgccg accggccagg agctgctgcg tcttctggag cagcggctgg
      841 acagcgtcgt ctaccggctc ggcttcgccc cgacccggcc gatggcgcgc cagttggtga
      901 accacggtca cgtgctggtc aacggccgcc gggtcgatat cgcctcgttc cgggtgccgc
      961 agggtgccac catcgcgctg aagccgatcg cggccgagat ccccgccgtg atcgaggcgc
     1021 tgaccgagac ctcgggccgg gtgccatcct ggctgtcgcg caacggcaac gagcccgagg
     1081 gccgggtcgt cggtccgccg gtggtcgagg agttcgacat gccggtggat gtcgagctcg
     1141 tgatcgcctt ctacacgcgc taatcaacca cgaggaagat agttcaacgg cgcgagcatc
     1201 gctcgcgccg tttgtcgtgg gtggctgcta cggggataga caagagcctt cgacaacgcg
     1261 ccgtacaggg cgacgagggc ggccacgggg gaacgcccct accgggagaa cgcggcgcct
     1321 atttccggta ggggcaggcc ccgtgcctgc cctcgctgcc cgccgcggtg ccttgtcgag
     1381 ggtcgcaaac ggcccattca cacacgggcc atggcgtata cttacgccct gacgggagcg
     1441 cattggtggg cgctcccgtt cgatctatac cgcggccagg tgctggagga ggagagcgac
     1501 agggtgggtt ttgcagcgag cgtcccggag ccgggaggct acagcgtcat cgggcgtcag
     1561 gtcgccctcg gcccgctccg ccgcgagctg gcgccgctct acgaggcctg gcgcggcaac
     1621 ttcaccacgc tacggcccct gttcgagctg ggagagtccg acgagctgga ggagcccgaa
     1681 ggctggtacg aggagatgac cgtctcggac gggcatgtct ggttcaccat ctacgagttg
     1741 gccggcggca agccgatcgg gctggtcggc ttgatcgaca tcgacttcga ggagaaggcc
     1801 gccgaattct tcctgcacgt gggcgaggtg gccagccagg ccgccgacct cggcagcgag
     1861 gccggccgcc tgatgcgcga ctacgccttt cacgcgctcg gcctcagcag cctcttctcg
     1921 atggcctacg agtacaacct ctccgagctg cgctactgcg agaccgccgg gtttcgcgag
     1981 ttcacccgcc agcgccaggc gcatctggtc ggcggccggc tctgggacgt gatctatctg
     2041 gagtgcctgg ccggcggccc agcagccacc ggccctgcat catcctgcta acccgtcgct
     2101 tccgtctacg agcccgccgg acgctcggcc agcgtaaccg gcacctgcat ggtctgtcca
     2161 ttgcgcacca ccgtcagcgt gagtgtgtcg ccgaccttat gactctcgac cgtgttggcg
     2221 aaggacgagt cggtcgtcaa ctcctggccg tcgaccttgg tcacgatgtc gcccttctgc
     2281 agccccgcct tggccgccgg cgagccgtct accacgctct ggaccagcgc gcccttcgtg
     2341 gccgtgacgt tgagctgcgc cgcgatggcc ggcgtcagcg ggatgtagct gaccccgacg
     2401 taggggtggg tcgccttgcc ggtgctgacg atctcgtcgg cgatctgctt ggcggtgttg
     2461 atcgcgatgg cgaagccgat gccctcggcc tgcacgcccg gctcggcctc accggccacc
     2521 agcgtgttga taccgatcac ctgaccgtcc aggttggtca gcgggccgcc cgagttgccg
     2581 gggttgatcg cggcatcggt ctggatcaca tccgagagcg tcggaccggc ctgtgactgg
     2641 cgcgagaagg ggccgctgct gttccctggc tcctgcaccg agcggttcag cgcgctgacc
     2701 acacccgccg tcaccgtcgg ctcgccctct aaccccagtg cgtagccgag cgcgaccacc
     2761 cattgcccga cttgcagctg cgacgagtcg ccgagctgca ccgccggcag gttgctgccg
     2821 ctgatctgca gcacggccag gtcggtcagg gggtcggtgc cgaccacctt gacgtcgttg
     2881 tccttgaagc tacggccgtc gggcagggtc acaaccagac tctgcgcacc ctcgaccacg
     2941 tggttgttgg tcaggatgtg cccctgattg tcatagatga atcccgagcc ggtgccgacc
     3001 ggaatcggct gcgcgagctg cgagacgctg gtctgctcgt tggtgatctc gaccaccgcc
     3061 ggcttggccg tcttggcgac ctgaatcacc gagttgttga agtcggcctg tgagggcgcg
     3121 ttgctggtgg tggtcttggt ggcgctggag gcgtcgctgg ccaacaccag tggcttggac
     3181 gtgctcgtcg cactaccggc ggtcgtgccg gtcgcggtgc tgccgctcga cgccgagctg
     3241 ccgctcagcg tgcacgccgc gccgaccgcg acgatcagca ccagtgccag gaacaacgcc
     3301 actacccgct ggccgccggg ccagaggccg cgcgcgccgc gcgctcggaa gggaagcatg
     3361 ggaatcctcc tcaaataggt tgaacggcca tccggccgcg aatcggggaa ccggtctcgc
     3421 cggttcgctg caactgtagc cagccaacct gaggagagac tgagcggcac ctgagcgctt
     3481 tctcataaag ctccaatgtt gagcctcgcg cccggagagc ggtggccggc gacaaggctg
     3541 ggaggcacag cacgacggat ccttcgaccg cgcacaggat gacggggtta atcgggggct
     3601 gacgacgagg acgtgacgta gtcgtacgcc tctacctctc cctatcatcc tgagcttgtt
     3661 gaaggatccg tcctggagcg gcactcaacg tcctcgacag gtgccggctc ctatccgccc
     3721 gcgtcgtgat cggccgcgcg cgcgtcgggc gcgagcgcca ccgtctccac cagtcgtgcc
     3781 gccaccgcgc ggagccggtc cagggcctga tcgtccggct cgggcagggg gccgagatgg
     3841 tggatgcgcg ccagcgcgat gaagcgcccg tagcgcgcgg cgatcgtatc gctgccggcg
     3901 aagataccgg tgaagttgcc cggctcgcgc gcctcgatgg ccgcctcgtc gagcgccggc
     3961 tcgtagccgg tgtcgaggag ataggtgcgc aggtgagtgc ggctgagccg gccgagccag
     4021 cccaccagct ccgccggggc cgccgccagg gccagcgcga tatcgtaggt gccgagcgtg
     4081 cgggtcagcc actcgaccag gtcggccccg gtgcgcggat cggtggtgcg gtagtcgatc
     4141 tccgggtggc gtgcgcgaaa gtcggccagc gcctgcccgc cggagcgccg cagcagcgcg
     4201 cgcaccgcgg gattggcgcg ctcctcgaag aaggtggcct ggtcgccgcg ttcggccagc
     4261 acctgcccca gcgcgcgcag gaggtaggca tcggggttgt cgaccgccga gacgaccgat
     4321 gcaggaaaga tggcccagcg cagccgggag ccgctcacct tgccggactc cgccgcgcgc
     4381 tacttgccgc catcacgcgt cgcgctagcg ctgcgggttc tcggtgcggt tctcgaagcc
     4441 cggccgcacc gccgagggat cgacctcggg gtggttgatc agattgacca cgttgcgcac
     4501 gcctggcacc ttgcgcgtac gctcctcgat ctccttgacc agcgtcgggt tgtcgacgtg
     4561 cccgcgcagg tagaccacat ggtcgaccgt gttgacgttg atctcgccct tgggcaggtc
     4621 gggatcgcgg aagacctcgc tcatgacccg gtcggtgatc gtctggctgt cgtcggtcca
     4681 gtgcgattgc ccgccgccca tggtctccat cgcctgatgc tgcaggccgc gcacgaagcc
     4741 ggacgcatag tcgagcttct gcggcagcgt gtcgctcgcg aaatcgcccg cgacattgcc
     4801 gaagtggacg aactgatcgc gcaccagcgc cctgcggcga ttgccggtgc gtgggtcgag
     4861 caggaacatg gcggcggcgc cggtcgccgc gccgagcagc ccccagcgca gcgggtggcg
     4921 ttgccggcgc tggcgccggg gcgcgcggct agccgcctcg ccggcgcgct tcttccagtc
     4981 gatttcaact ctcgctttgc gtgcgttcat cgtcggtccc tcccatgttg gctgcgtcgc
     5041 ggccttgacg ccgcgccagc gccggacgga tgccggcact ggtgcggccc acgttgcacg
     5101 acccgtgcca atccggctca ccccagcagg cagacatcga cgtcgccgct ggcctgcgcg
     5161 ctcagctcga catccgcgcc gttgccgcgc agcacgagat cgaccgtctg tccggcgaag
     5221 cgcaacccgt ccaggcgcac ctccggcagc caggcgggca ggtgcggccg cagccggagc
     5281 tgacggcgcg tggcgtccgg ctcgactccc agcagcgccg tcagcaggaa cgggatcgtg
     5341 ccggaggccc aggcctgcgg gctgcagctc accgggtagg cgaccgggat cgagacgcgc
     5401 tcggcgtcgc gctggaagcc gcagaagagc tcggggagcc gcaggtagtt gaagtggggc
     5461 acggcgtcga gcagatcgtt gaagaggcgc gcgaaggcgt cgtcggcgcc gtagcgccgc
     5521 agcc
//
LOCUS       DASUBI010000678         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_182289, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000678 DASUBI010000000
VERSION     DASUBI010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182289"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..731)
                     /locus_tag="VFU72_13120"
     CDS             complement(6..731)
                     /locus_tag="VFU72_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEU5424480.1"
                     /translation="MASIEQTRPIARPAREQLNLGAKLRALIQERYLPLGLLLLAAVL
                     LVSSIALPYWNLMLHAPQYPKGLPVTVYVTHLSGQVKEVDGLNHYIGMMKLNEAARFE
                     RAVAPFAIPVVALLVVASFWAPGRWKWLLVLPALIFPLVFIGDLAIWLYYAGHHLDKH
                     AALSSAIKPFTPHVFGVGRIGQFSTEASFGIGFYFIVLGALLATAATVIERRGLRRAR
                     PAAREQGELVATVSERGGSHGPR"
     gene            complement(803..2800)
                     /gene="nosZ"
                     /locus_tag="VFU72_13125"
     CDS             complement(803..2800)
                     /gene="nosZ"
                     /locus_tag="VFU72_13125"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEU5424481.1"
                     /translation="MNRISRRSLIKGAGAGIALVPAASALLASCSLPGGKSSKSEAGS
                     ADEEDIAKERKLTSDDLAAAAETYTPSGIHDPYYMFASGGHAGQVLVVGLPSMRILKE
                     IGVFTPEPWQGWGFSDDAKAVLDSGKVDGNAITWGDTHHPALSETNGAYDGQFLFIND
                     KAHARIGVIDLRDFVTKQIIKNPHIISDHAQFVTPNTEYLVEGSQYATPIGWEYAPIE
                     QYKEKYRGAMTFWKFDRAKGRIIPEESFTIELPPYDQDLGDAGKLVSDGWAFLNSCNT
                     EMAWGDDLNGKPSLEMGASQRDMDYLTVVNWKKAAEVVKAGKASQVKGMALISIDTAV
                     KEGLIFEIPEPKSPHGVDVAPKGDLVVIAGKLDPHVTVYSFQKIQDTIASGKFDKDDF
                     GIPILDFNSCLEAQVELGLGPLHTQFDDEGYAYTSLFLDSAVARWTLGGSYRKDGWKL
                     IEKLPVNYNVGHLAAIEGDTVNPQGKFLVSLNKWAIDRFAEVGPLHPQNFQLIDISGD
                     KMRLVYDMPIGMAEPHYAQIVRAETLKPWPTYPQSGWDPQTQSPSPNATTQHQEAVER
                     NGTTVTVKSWVMRSRITPDLVKIKQGDKVVWHVSNPETAEDATHGLAIPAHNINLSIE
                     PGATLTVSFNAEHAGVYPFYCTEFCSALHLEMMGYLIVEPT"
BASE COUNT          529 a         1057 c         1172 g          605 t
ORIGIN      
        1 cgaagctagc gcggcccatg gctccccccg cgctcgctga cggtggctac cagctcaccc
       61 tgttcccggg cggcgggccg cgctcgccgc agtccccggc gctcgatcac ggtggccgcg
      121 gtcgccagca gcgcgccgag cacgatgaag tagaaaccga tgccgaagct cgcctccgtc
      181 gagaactggc cgatccggcc caccccaaag acgtgcgggg tgaagggctt gatggcgctg
      241 ctgagggcgg cgtgcttgtc gagatggtgg ccggcgtagt agagccagat cgccagatcg
      301 ccgatgaaga cgagggggaa gatcagcgcc ggcagcacca gcagccactt ccagcgcccg
      361 ggcgcccaga acgaggcgac gaccagcagc gcgacgaccg ggatcgcgaa cggcgcgacg
      421 gcgcgctcga agcgggcggc ctcattgagc ttcatcatgc cgatatagtg gttgagcccg
      481 tcgacctctt tcacctgacc agagaggtgc gtgacataga cggtgaccgg cagacccttc
      541 ggatactgcg gcgcgtgcag catcagattc cagtagggca gggcgatcga gctgaccagc
      601 agcacggccg ccagcagcag caggcccagc ggcaggtagc gctcctggat gagcgcgcgc
      661 agcttcgcgc cgagattcaa ctgctctcgg gcgggacggg cgattggtcg tgtctgttcg
      721 atcgaggcca tcggacggca acccttcgtg atgcgaaccc ggccggcgga gcggctcgcc
      781 gccccgccgg ccggggcacg aactacgtcg gctcgacgat gaggtatccc atcatctcca
      841 ggtgcagggc cgagcagaac tcggtgcagt agaaggggta gacgcccgcg tgctcggcgt
      901 tgaaggaaac agtgagcgta gcccccggct cgatgctcag gttgatgttg tgggcgggta
      961 tggcgagtcc gtgcgtcgcg tcctcggccg tctcgggatt ggagacgtgc cagacgacct
     1021 tgtcgccctg cttgatcttg accaggtcgg gcgtgatgcg gctgcgcatc acccaggact
     1081 tgacggtgac cgtggtgccg ttgcgctcca cggcctcctg atgctgggtc gtcgcgttgg
     1141 gcgacggcga ctgcgtctgt gggtcccagc cggactgcgg gtaggtgggc cagggcttga
     1201 gcgtctcggc gcgcacgatc tgcgcgtagt gcggctcggc cataccgatc ggcatgtcgt
     1261 agacgagtcg catcttgtcg ccgctgatgt cgatgagctg gaagttctgc gggtggagcg
     1321 ggccgacttc ggcgaagcgg tcgatggccc acttgttcag cgacaccagg aacttgcctt
     1381 gcgggttgac cgtgtcaccc tcgatcgccg ccaggtggcc gacgttgtag ttgaccggca
     1441 gcttctcaat cagcttccag ccatccttgc gatagctgcc gccgagcgtc catcgcgcca
     1501 cggcgctgtc gaggaagagg ctggtgtagg cgtacccctc gtcgtcgaac tgggtatgca
     1561 gcgggccgag gccaagctcg acctgcgctt ccaggcaact gttgaagtcg aggatgggga
     1621 tgccgaagtc gtccttgtcg aacttgccgg aggcgatcgt gtcctggatc ttctggaagc
     1681 tgtagaccgt cacgtggggg tccagcttgc cggcgatcac gaccaagtcg cccttgggcg
     1741 ccacgtcgac gccgtggggg ctcttgggtt cggggatctc gaagatgagc ccctccttga
     1801 ccgccgtgtc gatcgagatc agcgccatcc ccttgacctg gctggccttg ccggccttga
     1861 cgacctcggc cgccttcttc cagttgacga cggtcaggta gtccatgtcg cgctgcgagg
     1921 cgcccatctc cagcgagggt ttgccgttga gatcgtcgcc ccaggccatc tcggtgttgc
     1981 aggagttcag gaaggcccag ccgtcgctga ccagcttgcc ggcgtcgccg aggtcctgat
     2041 cgtagggcgg cagctcgatg gtgaacgact cctccgggat gatgcgaccc ttggcgcggt
     2101 cgaacttcca gaaggtcatc gccccgcggt acttctcctt gtattgctcg atcggcgcgt
     2161 actcccagcc gatcggggtg gcgtactgcg agccttcgac gaggtactcg gtgttcggcg
     2221 tgacgaactg ggcgtggtcg ctgatgatgt gtggattctt gatgatttgc tttgtgacaa
     2281 aatcacgcaa gtcgatcacg ccgatgcggg cgtgcgcctt gtcgttgata aagaggaact
     2341 ggccgtcgta ggcgccgttg gtctcgctga gcgccgggtg gtgggtgtcg ccccaggtga
     2401 tcgcgttccc gtcgactttg ccgctgtcga gcaccgcctt ggcgtcgtcg gagaaccccc
     2461 agccctgcca gggctcgggc gtgaagacgc cgatctcctt gaggatgcgc atcgacggca
     2521 ggccgaccac cagaacctgc ccggcgtgac cgccggaggc gaacatatag taggggtcgt
     2581 gtatgccgct gggggtatag gtctccgcag cggcagccag gtcgtcggag gtgagcttgc
     2641 gttccttggc gatgtcctcc tcgtcagcgg agcccgcctc gctcttggag ctttttcccc
     2701 caggaagact gcacgatgcg agcagcgcgc tcgcggccgg aacaagagct attcccgctc
     2761 ctgctccttt gatcagggat cgccggctta tccggttcat tgctccccct tgtccatgag
     2821 catttcaggt ccaccagccg gccgatgtcg cgcgccagtt gtggccggca agagaccggc
     2881 tatcgtcagg cataaggcta gagatttgag acagccggct ccagaccgga gtgggtagtc
     2941 ccggtcttac tggatgagtg ggttcggaat cgcctgccta cccgagtggg tagggcagat
     3001 agcttcgcgc tgacttagta tagttggtaa cgatcaagtc ggagggagac acaagctggt
     3061 cgccggctgc cgcgaccgct gcggcgaagg cggcgcggca cccgcgcttc ccgctcgtgc
     3121 gcccgcgcga cggcaggatg ccgccgggcc cgatcgccac gggtatcacc gaacgcagcc
     3181 acggccggtc ggggcggcag gtcgcgcggc gccggggagc gggcgtgccg cgggcggcgg
     3241 ccgccgttcg ccgcccgcag ccctaggcca gcttgacctc cggcacggcg cggccggcgt
     3301 cgtcgacggc gaacagctcg gcgggggcga agccgccggc catcacgctg gccgggaaga
     3361 cct
//
LOCUS       DASUBI010000679         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1054761, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000679 DASUBI010000000
VERSION     DASUBI010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054761"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..337)
                     /locus_tag="VFU72_13130"
     CDS             complement(14..337)
                     /locus_tag="VFU72_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424482.1"
                     /translation="MAKASGLSDQVDTDLCAIAAELEFLPEMGEFWPEEPEGSRVSYS
                     LEWDELMRRLIHLSHLERSEKLSPDQTRRLHALLRTFQALTPTIERLGLYRPAIGATF
                     TKDCR"
     gene            955..1779
                     /locus_tag="VFU72_13135"
     CDS             955..1779
                     /locus_tag="VFU72_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5424483.1"
                     /translation="MRIVGWNIRAGGGRRVELIAAQLDAWAPDIVALSEFRATPPSQH
                     LAEALATRGLAFQQAALDPDQLSRNGLLVASRWPLKPIRARSAPSEPCRWLLVGVDAP
                     APFTLGAMHIPVSVSGRKLPYLGAVERLVRSWRRGPGLLIGDTNSGRPGIDEESPVFG
                     PRSAAWFDELDRRGWADAFRHLHGDTRVYTWYSPNAGNGFRLDQAFVNRHLLPRLRQA
                     RYEWGQSLESVDPAARTTLSDHAALLLDFDLYDGRRAQGNEALASYPCQDLAPRAL"
     gene            2365..>2733
                     /locus_tag="VFU72_13140"
     CDS             2365..>2733
                     /locus_tag="VFU72_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424484.1"
                     /translation="MAPVALAQGTPSITVVSPTPNQKITTTDIPLQVKTSGIDTACKW
                     VGTPDQPGQGNIHVFLDKASLGALINFYCGTDSITVSGEGITPGPHTLIVDLASNTHG
                     DMLDTAQKVAIDYEPATPKPL"
BASE COUNT          507 a          930 c          862 g          434 t
ORIGIN      
        1 caggcttgcc agatcaacgg caatctttcg tgaatgtcgc gccgatagcc ggccgataga
       61 gaccaagccg ttcgatcgtg ggtgtcagtg cttggaatgt ccgcagaagc gcatgcaagc
      121 gtcgcgtctg atcgggagag agcttttccg accgctcaag gtgactgagg tggataagtc
      181 gtcgcatcag ttcatcccac tccagcgagt agctgacccg gctgccctcg ggctcctccg
      241 gccagaactc ccccatctca ggcaagaact caagttcggc cgcgatcgca cagagatcgg
      301 tatcaacttg atcgctcagc ccggaagcct tagccatcgc acatcactcc gctccggatg
      361 gacgagaaca tccagcccat tctcgggctg aaacgcggtt gtccaatgcc ccgtcgctag
      421 tcgatattct atcaagatcc actcgtgccc ccacggaccg cgccatgaag cgctcctacg
      481 gattatgccg agctgccatc ctccgtgata tcggccggtg aagacgccgc ttagggggtc
      541 ggctataatc tgtgcaagac tcgctagata accaccgaaa gttgttccga cgggccattc
      601 ggccatcgcg acgacatggc gcaagtaatg cacctcggca ggcgggagcc gatcacggcc
      661 tgcgagcgtc cgacctcccc attcgatccc gcgtaggcgg ccaccagcac gctccagcgc
      721 gtccgggtcg aacccggctc gtgcgacgtg gtcgagaacg cggtccaagt cagccgtgtt
      781 caggcgccgc gtgccggccg cggccgcggc gatcaactgg tcggcctcag actccacgac
      841 caccctgccc ttgatcccgt aggataggca ccggcgcacc tcaactcttc accatcgatg
      901 agaagctggg acgcgcagat cagacgatta tcaggaacgt ccggggcgag atcaatgcgc
      961 attgtcggct ggaatatccg cgcgggcggc gggcgacgag tcgagctgat cgccgcgcag
     1021 ttggacgcct gggcgccgga tatcgtggcg ctctccgagt tccgcgcgac gccgccgagc
     1081 cagcacctgg cagaggcgct ggccacgcgg gggctggcct ttcagcaggc cgccctcgac
     1141 cctgatcagc tgagccgcaa cgggctgctg gtcgcctcgc gctggccgct gaagccgatc
     1201 cgggcgcgat ccgcgcccag cgagccctgc cgctggctcc tggtgggagt cgacgcgcca
     1261 gcacccttca cgctcggtgc gatgcacatc ccggtgtcgg tcagcgggcg caagctgccc
     1321 tacctcggcg cggtggagcg tctcgttcgc tcctggcgcc gcgggccggg tctgctgatc
     1381 ggcgacacca actccggccg gcccggtatc gacgaagaat cgcccgtctt cggaccgcgc
     1441 agcgccgcct ggttcgacga gctcgaccgg cgcggctggg ccgacgcctt ccggcacctg
     1501 cacggcgaca cccgcgtcta cacctggtac tcacccaacg ccggcaacgg ctttcggctc
     1561 gaccaggcgt tcgtcaatcg ccacctgctg ccacggctgc gccaggcacg ctatgagtgg
     1621 ggccagtcgc tggaatccgt cgacccggca gcgcgcacca ctctcagcga ccacgccgca
     1681 ctcctgctgg acttcgacct gtacgacggg cgtcgcgccc agggtaacga ggctctagcg
     1741 agctatccat gccaggatct tgcgccgcgg gcgctgtgac cagcgtcgtc accacctcgc
     1801 aacgcaccac cgggagatgg gcggctgggc gcgccacagt gcgggacaga gcgagcgccc
     1861 agcacagcgc ggtgacagcg ccggttcccc cgcgcagggg gccaaccacg cgaacgggac
     1921 tgacgtcgcg caacgggcgc tgatgctagg cccgaataat gctgcgctcc gcgtcacgga
     1981 cgtcagcatc gacgtcgacc tagagagatc caataaatca agaaaatgct tgacttattc
     2041 gccacgacgt gctattggtg gagtgaggta gcggaacgcg ccggccgccc gcgcggcagc
     2101 gaacagccgc tggccgggcg cgagaggagg cagcgacagt cgcggacggt gcgatgccgg
     2161 ggaatatgat cggaatacgt cggccgaaac aggtcggaag gagatggctc agtgcacata
     2221 ccatttcggt cccaagatcg tccagcctat cggagcggca gtcacgaatc gccggctatg
     2281 gcggcggcaa gggtgcacgg gcgatggacc gtcatccggc acgcgttgag cctcgtggcg
     2341 acgttcgcga tggttgcgct ctttatggct ccggttgcac tggcgcaggg gacaccgtcg
     2401 atcacggtcg tcagcccgac gccgaaccag aagatcacca ccactgatat cccgctccag
     2461 gtcaagacga gcggcatcga taccgcctgc aaatgggtcg gtacgcccga ccagccgggc
     2521 cagggcaaca tccacgtctt cctggataag gcgagcctgg gtgcgctgat caacttctat
     2581 tgcggcaccg acagcatcac ggtctccggc gaggggatca ccccgggtcc ccacaccttg
     2641 atcgtcgatc tcgccagcaa cacccacggc gacatgctcg acaccgccca gaaggtggcg
     2701 atcgactatg agccggcgac gccgaagcca ctg
//
LOCUS       DASUBI010000680         4403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_156952, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000680 DASUBI010000000
VERSION     DASUBI010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156952"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="sat"
                     /locus_tag="VFU72_13145"
     CDS             complement(<1..1019)
                     /gene="sat"
                     /locus_tag="VFU72_13145"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEU5424485.1"
                     /translation="MANDTPGILIAPYGGHLVDLLVPEDQREALHAYAGGLPSVQLSD
                     RSVCDLELLATGAFSPLDRFVGQRDFQGVLDEMRLASGVVWPMPITLPVEPAPAIELG
                     ADIALRDSKNNLLAVMTIEEIYEWDRDEVADKVFRTRDLRHPLVAEMSRWGRLNISGP
                     LKVLALPQHFDFQDLRRTPAQTRARLEEIGNPNVVAFQTRNPLHRAHEELTKRAIEAV
                     NGVLLLQPVVGMTKPGDVDHFTRVRTYRALAANYYDPSRILLSLLPLAMRMAGPREAL
                     WHAIIRRNHGANHFIVGRDHASPGIDSTGKPFYEPYAAQENMKKYQGEIGMQMVDFKM
                     MVYLP"
     gene            complement(1288..1968)
                     /locus_tag="VFU72_13150"
     CDS             complement(1288..1968)
                     /locus_tag="VFU72_13150"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEU5424486.1"
                     /translation="MSWLAYLTVGMPVYALDGPLGVITSVPRVDLDDPDAPAEVIVLA
                     NVETASRGVEEFLRVTRPMVDRVQNGALYLGVSRRDVPPASASVAGTQRLRGTAAGLT
                     IPIVEEQVEVQTRVVDLGHVHVRKEVEQQLDERTLPLRHQEMQVEHVPVDEIIPEMLE
                     PYLDGDAYVVPIIEEEVVIERRLRLKELLRITRVAAEHEQTLRTPYRRERVVVEEHWH
                     GDAPPGRQ"
     gene            complement(1979..2596)
                     /locus_tag="VFU72_13155"
     CDS             complement(1979..2596)
                     /locus_tag="VFU72_13155"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEU5424487.1"
                     /translation="MIWNEPAQAERRVWEIDDGWDVVASDGARLGEVADVRGSYIVVE
                     KGLLFKTDLYIPVAAIVAVAQRRVHLGLSGEAIAQQGWDQPPTKVARAPTRRLPRLEE
                     PAAPPAEPLLLVQPGAPEAELPVDAPLRLEEIRVERRTVDPPVAAEGLVAPFQELEIV
                     IPIRGEEAVVVTRPVVREEVVIRRGARERTELSDLSAESPGLAWK"
     gene            2705..3238
                     /locus_tag="VFU72_13160"
     CDS             2705..3238
                     /locus_tag="VFU72_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424488.1"
                     /translation="MEPLNIDLQPAPLRERPRLGFLYRAHFADPRRERLFLSSLSFFL
                     TLVIVRGVTHAIRAGIGPFHNITTGALHIHPLGPGILLLLAVGYLQLLQPPVQHRLWL
                     DIARLLALLYGVGAGLTLDEFALWLHLQDVYWLPQGHESWDAIELFFSVLSIGWWGGP
                     FFRALGRDTLRFLRRID"
     gene            3384..>4403
                     /locus_tag="VFU72_13165"
     CDS             3384..>4403
                     /locus_tag="VFU72_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424489.1"
                     /translation="MRFSPRTALRTLPVVALVLFTAAIPATSAAAALSAADPSFSRVW
                     QLTDQPVVDGVSRSWLWGPRADTAGIQERYLESPGGQRLVQYFDKGRMEINDAGADPG
                     GEWYVTSGLLDRELISGKIQVGDDRYLDTGAGAHIPVAGDPGNLFPTYADLQRVVDRG
                     QPDETGSPATVVLTPTGQGSLPGAAADPNAQFVHYARYIGPAGQPVGYNIPRAFWDYM
                     NQAGPVDDGGTTRQAAPLFDWLFTLGWPIADPFWTQVSLAGASQWVLVQPFERRLLTY
                     TPANPDGWKVEMGNIGLHYLRWRYAQTPPVQISGDIAFLAMQQGNQWSYSTSAGATEE
                     WRAGGT"
BASE COUNT          635 a         1622 c         1447 g          699 t
ORIGIN      
        1 tcggggaggt agaccatcat cttgaagtcg accatctgca tgccgatctc gccctggtac
       61 ttcttcatgt tctcctgggc ggcgtacggc tcgtagaagg gcttgccggt cgagtcgatg
      121 ccgggactgg cgtggtcgcg cccgacgatg aagtggttgg cgccgtggtt gcgccggatg
      181 atcgcgtgcc agagcgcctc gcgcggcccg gccatgcgca tcgccagcgg cagcagcgag
      241 agcaggatgc ggctgggatc gtagtagttg gcggccagcg cgcggtaggt gcgcacgcgc
      301 gtgaagtggt cgacatcgcc cggcttggtc atgccgacga ccggctgcag cagcagcacg
      361 ccgttgaccg cctcgatggc gcgcttggtc agctcctcgt gggcgcggtg cagcgggttg
      421 cgcgtctgga aggcgaccac gtttgggttg ccgatctcct ccagacgggc ccgcgtctgg
      481 gccggcgtgc ggcgcagatc ctggaaatcg aagtgctgcg gcagcgccag caccttgagc
      541 gggccggaga tgttcagccg gccccagcgc gacatctcag ccaccagcgg gtggcgcagg
      601 tcgcgcgtgc ggaagacctt gtcggccacc tcgtcgcggt cccactcgta gatctcttcg
      661 atcgtcatca cggccagcag gttattcttg ctgtcgcgca gggcgatatc ggcgcccagc
      721 tcgatggcag gtgccggctc gaccggcagc gtgatcggca tcggccagac aaccccgctc
      781 gccaggcgca tctcgtccag aacaccctga aaatcgcgct gacccacgaa gcgatcgagc
      841 ggcgagaacg cgccggtcgc cagcagctcc agatcgcaga cggagcgatc ggagagctgc
      901 acggagggga gcccgccggc gtaggcgtgg agcgcctcgc gctgatcctc cggaaccagc
      961 aggtcgacga ggtgtccccc gtacggcgcg atcaaaatac ccggcgtgtc gttagccacg
     1021 tctcttccct tcccggcgac gctgagccaa tcctattgcg taacgcatag attcttcctg
     1081 atcgaccacc atcaggtcaa cctcccatcg taccggttcc ggtatcggtt ccgaacgatg
     1141 ggaaccgcgt gtgcttccac tgtccaggat ggcggttgag caaggatgga gaaacgagtg
     1201 cggcgtcgta aagggtaggc ataggcaacg agggtggccc ggctcggcgc ggggccaccc
     1261 tccgaaggcg tgattggccg gcggcgccta ctgccggccc ggcggggcgt cgccgtgcca
     1321 gtgctcctca accaccacgc gctcgcggcg gtagggcgtg cgcagcgtct gctcgtgctc
     1381 ggcggccacc cgcgtgatgc gcagcagctc cttgagccgc aggcgccgct cgatcacgac
     1441 ctcctcctcg atgatcggca ccacgtaggc gtcgccgtca aggtacggtt cgagcatctc
     1501 cgggataatc tcgtcgaccg gcacatgctc gacctgcatc tcctggtggc gcagcggtag
     1561 ggtccgctcg tcgagctgct gctcgacctc cttgcgcacg tgcacgtggc cgaggtcgac
     1621 cacccgtgtc tgaacctcga cctgctcctc gacgatcggg atcgtcagcc ccgccgccgt
     1681 gccgcgcagg cgctgtgtgc cggccaccga ggcgctggcc ggcggcacgt cgcggcgcga
     1741 caccccgaga tagagcgcgc cgttctgcac gcgatcgacc atcgggcgcg tcacccgcag
     1801 aaactcctcg accccccggc tggcggtctc gacgttcgcg agcacgatca cctcggccgg
     1861 cgcgtccgga tcgtcaagat cgacgcgcgg caccgaggtg atcaccccca gcgggccatc
     1921 cagcgcgtag accggcatgc cgacggtcag gtaggccagc cagctcatcg cgacctcctc
     1981 acttccaggc caggccgggc gactcggcgc tcaggtccga caattccgtc cgctcgcgtg
     2041 ccccgcgccg gatcaccacc tcctcgcgca ccaccggccg cgtcaccacc acggcctcct
     2101 caccgcgaat cgggatgacg atctccagct cctgaaaggg cgccaccagc ccctcggcgg
     2161 ctaccggcgg gtccaccgtg cgccgctcga cgcggatctc ctccaggcgc agcggggcat
     2221 cgaccggcag ctccgcctcg ggcgcccccg gctggacgag cagcagcggc tcggccggcg
     2281 gcgctgccgg ctcctccagc cgtggcagcc gccgcgtcgg ggcccgcgcg accttggtgg
     2341 gcggctggtc ccaaccctgc tgcgcgatcg cctcgccgct cagcccgagg tgcacgcgcc
     2401 gctgcgccac cgccacgata gccgccaccg ggatgtagag atcggtcttg aagagcagcc
     2461 ccttctcgac cacgatgtag ctgccacgca cgtcggccac ctcgccgagc ctggcgccgt
     2521 ccgaggcgac cacgtcccaa ccgtcgtcga tctcccagac ccggcgctcg gcctgggctg
     2581 gctcattcca gatcacccgc accctccgtt cgttgcacgc cggcgctcgc ccagcgtcag
     2641 gtaggcgcgg ggcgagcaag ggatgtgcca cagcgcgtat actggcccgg cggtgaggaa
     2701 ccagatggag ccactcaaca tcgacctgca gccggcgccg ctccgggaac ggccgcggct
     2761 gggctttctc taccgcgcgc acttcgccga tccgcgtcgc gagcgcctct tcctctcctc
     2821 gctgagcttc ttcctcaccc tcgtgatcgt gcgcggcgtc acccacgcca tccgggccgg
     2881 gatcgggccg ttccacaaca tcaccacggg tgcgctacac atccaccccc tggggcccgg
     2941 catcctgctg ctgctcgcgg tcggctacct gcaactcctg cagccgcctg ttcagcaccg
     3001 gctctggctc gacatcgcgc ggctgctggc gctcctctac ggcgtcggcg ccgggctgac
     3061 gcttgacgag ttcgccctct ggctgcacct gcaggacgtc tactggctgc cccagggcca
     3121 cgagagctgg gacgccatcg agctcttctt ctcggtcctc tcgatcggct ggtggggcgg
     3181 ccccttcttc cgcgccctcg gccgcgacac gctgcgcttc ctgcggcgca tcgactgatg
     3241 gtcgccggcc gccgcggccc acgccgcgcc tgcgtggaat ctgctagact gtctgcgtcg
     3301 ttgtcgtccc tgccgaccca cctttccccg gttcgtctgc cacccgcacg cgctttggtg
     3361 gggagcgatt ggatccggca tccgtgaggt tctcgccgcg caccgcgctg cgtacattgc
     3421 ccgtggtcgc gctcgtgctc ttcaccgccg ccatcccggc cacatccgct gccgccgcgc
     3481 tgagcgcggc cgatccgtcc ttcagccgcg tctggcaact gaccgaccag ccggtggtgg
     3541 atggcgtctc gcggagctgg ctgtgggggc cgcgcgcgga tacggcgggc atccaggagc
     3601 gctatctgga gtcgccgggc ggccagcggc tggtgcagta cttcgacaag ggccgcatgg
     3661 agatcaacga cgccggcgcc gatccgggcg gcgagtggta tgtcaccagc ggcctgctcg
     3721 accgtgagct gatcagtggc aagatccagg tcggtgatga ccgctacctg gacaccggcg
     3781 cgggcgcaca catcccggtc gccggcgacc ccggcaacct gttcccgacc tacgccgatc
     3841 tccagcgcgt cgtcgaccgc ggacagcccg acgagaccgg cagcccagcc accgtcgtcc
     3901 tgacgcccac cgggcagggc agtctgccgg gggccgctgc cgaccccaac gcgcaattcg
     3961 tccactacgc gcgctacatc ggccccgccg gccagccggt cggctacaac atcccgcgcg
     4021 ccttctggga ctacatgaac caggccgggc cggtcgacga cggcggcacg acccgccagg
     4081 ccgcgcccct cttcgactgg ctgttcacgc tcggctggcc catcgccgac ccgttttgga
     4141 cgcaggtcag cctggccggc gcgagccagt gggtgctggt gcagcccttc gagcgccggc
     4201 tgctgaccta tacccccgcc aaccccgacg gctggaaggt cgagatgggg aacatcgggc
     4261 tgcactacct gcgctggcgc tacgcccaga cgccgccggt gcagatcagc ggcgatatcg
     4321 ccttcctggc catgcagcag gggaatcagt ggtcgtactc gacctcggcc ggcgccaccg
     4381 aagagtggcg cgcgggtggg acg
//
LOCUS       DASUBI010000681         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_544191, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000681 DASUBI010000000
VERSION     DASUBI010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544191"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VFU72_13170"
     CDS             <1..1528
                     /locus_tag="VFU72_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU5424490.1"
                     /translation="LVLLVVFIGGTSSIAIEIAASRLLGPYFGTSTFIWANLIGMTLL
                     YLSVGYYVGGRIADRWPSPRLLLAITAVAGIGTGLIPPLSRPILERSLTAFANLSVGA
                     FYGSLVGVILLFAIPVTLLGFVSPFAIRLRMRRVASAGNTAGSLYALSTVGSILGSFL
                     PVLLLIPTVGTHWTFYIFALALLVTAALGLLLLRAFWFGGLVSVAAAVLLVYASFANH
                     GVVRPSEWGTLVHEEESQYNYIQVEKQNGTYLLVLNDGHAVHSIYDPTATFYNGYWDD
                     FGLAPFFNGGETPGDVKSAALIGLAAGTVVKQLNRSFGPIPIDGVEIDPDIVKLGRQY
                     FDMNEPNLDVYVQDGRYFLETSDRRYDLIGIDAYQQPYIPFHLVTREYFQEVSDHLSD
                     NGVVVLNAGRTETDFRLVNVIAATMKSVFPNVYIFDDNYYTNSVVVATKSPSNIANFA
                     RNIQQLQPGFLKTVGERVLQTGNVREWTQQDPALVFTDDKAPVERIVNRMIVDAARGE
                     "
     gene            1518..2315
                     /gene="pgeF"
                     /locus_tag="VFU72_13175"
     CDS             1518..2315
                     /gene="pgeF"
                     /locus_tag="VFU72_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU5424491.1"
                     /translation="MASDGVFRFASLSRPGLRHGITGRDPRLPAEGDVSFVTSPDTET
                     VRRNRQVWCARIGVDWRLITTAQQYHGDRVQVVRAQDAGRGAESSRDGFPGSDSLITR
                     ELGVPIMVVSADCVPILLYDPVRKAIGAVHSGWRGTVAGVVMRTVEAMRDEFGCRPAD
                     LMVGLGPSIGPCCYEVGPEVIAAWEALGVDPERRAVVERQPRPYFDLWTANELTLGAV
                     GVPAANIERAGLCTRCLSERYFSRRAGVGYRGLFGALIALGVEEEPQ"
     gene            2312..2989
                     /locus_tag="VFU72_13180"
     CDS             2312..2989
                     /locus_tag="VFU72_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5424492.1"
                     /translation="MSIESNLRQVNETIAQAAECAGRAPSSVRLIGVSKTVGRGQVDE
                     AYAAGLRDFGENRVQDAKEKFNPPLSAGATLHLIGYLQTNKARDAAALFDYVHSVDRL
                     AIIEALNKRAAQLDKHLKVLVQVNIAGEEQKHGCEPEDAAGLVRAAGACSHLEVVGLM
                     TIAPLVATSEEARPVFRGLRELRDEIARSTGIELSELSMGMTNDYGVAIEEGATMIRV
                     GRAVFHS"
     gene            2999..>3757
                     /locus_tag="VFU72_13185"
     CDS             2999..>3757
                     /locus_tag="VFU72_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424493.1"
                     /translation="MRVTCLASGSSGNCLLVQSGSTHVLLDAGVGVRVARKELAARDV
                     ADHALSAILISHEHNDHVRALPSLLGYQRAPVLATRGTLAALQPWISAEYDALTPGLA
                     CEIGELCVTPVRVSHDATEPVGFVFEAAGVRVAVFTDLGCVDGDVASAICGAALVVLE
                     SNYDEQLLARGSYPAHLKRRIRGPQGHLSNADCARFLAAHLSFETGDVWLAHLSQNNN
                     RPPLARGATQASLGPGGPQVRTLPRHGASVTWDSS"
BASE COUNT          577 a         1270 c         1240 g          670 t
ORIGIN      
        1 actcgtgctg ctcgtggtct tcatcggcgg cacctccagc atcgccatcg agatcgccgc
       61 ctcgcgtctg ctggggccat acttcggcac gtccacgttc atctgggcca atctcatcgg
      121 catgacgctg ctctacctct cggtcggcta ctacgtcggc gggcgtatcg ccgatcgctg
      181 gccgagcccg cgactgctcc tggccatcac cgccgtcgcc ggcatcggca ccggtctgat
      241 cccgccgctc tcgcgaccga tcctggagcg ctcgctgacc gccttcgcca atctctcggt
      301 cggcgccttc tatggctcgc tggtcggcgt catcctcctc ttcgccatcc cggtcacgct
      361 gctcggcttc gtctcgcctt tcgccatccg actgcgcatg cggcgggtcg cctcggccgg
      421 caataccgcc ggctccctct acgcgctctc caccgtcggc agcatcctgg gaagtttttt
      481 gcccgtactg ctgttgatcc cgacggtcgg cacgcactgg actttctaca tcttcgcgct
      541 cgcgctgctg gtcaccgcgg cgctcggcct gctgctgctg cgcgccttct ggttcggcgg
      601 ccttgtgtcc gtggcggcgg cggtgttgct ggtctacgcc tccttcgcca accacggcgt
      661 cgtgcgtccc tctgaatggg gcacgctggt gcacgaggaa gagtcgcagt acaactacat
      721 ccaggtggag aagcagaatg gaacctacct cttggtgttg aacgacgggc acgccgtcca
      781 ctcgatctac gatcccaccg ccaccttcta caatggctac tgggacgatt tcgggctggc
      841 gccgttcttc aacggcggcg agacgccggg cgacgtcaag agcgcggcgc tgatcggtct
      901 ggcggcgggc accgtggtca aacagctcaa ccgctcattc ggaccgattc cgatcgacgg
      961 ggtcgagatc gatccggaca tcgtcaagct cgggcgccag tacttcgaca tgaacgagcc
     1021 gaatctcgac gtctacgtgc aggacggccg ctatttcctg gagacgtccg accggcgcta
     1081 tgacctgatc gggatcgacg cgtaccagca gccctacatc cccttccacc tggtcacgcg
     1141 tgagtacttt caggaagtct cggatcattt gagcgacaat ggtgtggtcg tgctgaacgc
     1201 gggacgcacc gagaccgact tccgcctggt caacgtgatc gccgccacca tgaagagcgt
     1261 ctttccgaat gtctacatct tcgacgacaa ttactacacc aatagcgtcg tggtggcgac
     1321 caagtcgccc agcaacatcg ccaatttcgc ccgcaacatc cagcaactgc aacccggctt
     1381 cctcaagacg gtcggcgagc gcgtgctgca gaccggcaac gtccgtgagt ggacacagca
     1441 agacccggcg ctggtcttca ccgacgacaa agcgccggtc gagcgcatcg tcaaccgcat
     1501 gatcgtggat gctgcccgtg gcgagtgatg gcgtcttccg ctttgcgtcg ctgagccggc
     1561 ccggcctgcg ccacggcatc accggccgcg atccgaggct tccggcggag ggtgatgtca
     1621 gcttcgtgac cagcccggac acggagacag tacggcggaa tcgccaggtt tggtgcgctc
     1681 ggatcggcgt cgactggcgt ttgatcacca cggcacagca gtatcacggc gaccgcgtgc
     1741 aggtcgtgcg cgcgcaggac gccgggcgcg gagcggaatc ctcccgtgac ggctttcccg
     1801 gcagcgactc gctcatcacg cgcgagctcg gcgtcccaat catggttgtc tctgccgact
     1861 gcgtgccgat tctcctgtat gatccggtgc gaaaggctat cggggccgtc cactcaggct
     1921 ggcgcggtac ggtggccggg gtcgtcatgc gcacggtcga agcgatgcgc gatgagtttg
     1981 ggtgccggcc ggcggacctg atggttggac tggggccgtc gatcgggccc tgctgctacg
     2041 aagtcggtcc ggaggtgatc gcggcctggg aggcgctggg tgtcgatccg gagcggcgcg
     2101 cggtggtcga gcgccagcca cgtccctact tcgacctgtg gaccgccaat gagctgacgc
     2161 tgggcgcggt gggggtgccg gccgcgaaca tcgagcgcgc cggactctgc acccgctgcc
     2221 tgtccgagcg ctacttctcc cgccgcgccg gcgtcggcta tcggggcctg ttcggcgcgc
     2281 tgatcgctct gggggtggag gaagagccgc aatgagcatc gagagcaact tgcgccaggt
     2341 caacgaaacg atcgcccagg ccgccgagtg cgccgggcgc gcgccgtcga gcgtgcgcct
     2401 gatcggcgtg agtaagacgg tcggacgcgg ccaggtcgac gaggcctacg ccgccggcct
     2461 gcgcgatttc ggcgagaacc gcgtgcagga tgccaaagag aagttcaatc cgccactgtc
     2521 ggcgggcgcc acgctccacc tgatcggcta cctgcagacc aataaggcca gggacgccgc
     2581 cgcgctcttc gattacgtcc actcggtcga ccgcctggcg atcatcgagg cgctgaataa
     2641 gcgcgcggcc cagctcgaca agcacctcaa ggtgctggtg caggtcaaca tcgcgggcga
     2701 ggagcagaag cacggctgcg agccggagga cgcggccgga ctggtgcgcg cggccggcgc
     2761 gtgcagccac ctggaggtgg ttggcctgat gacgatcgcg ccgctggtgg cgacctcgga
     2821 ggaggcgcgc ccggtctttc gcggcctgcg cgagttgcgc gacgagatcg cgcgctcgac
     2881 cgggatcgaa ctgtccgagc tctcgatggg catgaccaat gactacgggg tcgccatcga
     2941 ggagggcgcg accatgatcc gggtcggccg cgccgtcttt cacagttagg tcgaacctgt
     3001 gcgcgtcacc tgtctggcga gcggcagcag cggcaactgt cttctggtgc agagtgggag
     3061 cacgcacgtc ctgctcgacg ccggtgtcgg cgtgcgcgtg gcccgcaagg agctggcggc
     3121 ccgtgacgtg gctgatcacg cgctgagcgc catcttgatc tcgcacgagc ataacgatca
     3181 cgtgcgagcg ctcccgagcc tcctgggcta tcagcgagcc cccgtcctgg ccacacgcgg
     3241 cacgttggcc gcgctgcagc cgtggatcag cgccgagtat gacgcgctca cgcccggcct
     3301 ggcctgcgag atcggcgagc tgtgcgtcac cccggtgcgg gtctcgcacg atgccaccga
     3361 gccagtcggc ttcgtattcg aggccgcagg tgtgcgcgtg gcggtcttca ccgatctcgg
     3421 ctgcgtcgac ggcgacgtgg cctcggctat ctgcggcgcg gcgctggtgg tgctggaatc
     3481 gaactacgac gagcagctgc tggcgcgagg gagttacccc gcccacctca agcgacgcat
     3541 tcgtgggcca caggggcact tgagcaatgc ggactgcgcc cgcttcctgg ccgcgcatct
     3601 ctcgtttgaa accggcgacg tctggctggc gcacctctcg cagaacaata atcgcccgcc
     3661 gctggcccgc ggcgcgacgc aggcgagcct gggtcccggc ggccctcagg tccgcaccct
     3721 tccccgtcac ggcgcctcgg ttacctggga ctcgagt
//
LOCUS       DASUBI010000682        10165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_331944, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000682 DASUBI010000000
VERSION     DASUBI010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331944"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFU72_13190"
     CDS             <1..517
                     /locus_tag="VFU72_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5424494.1"
                     /translation="DPPTAIAVARQLEAYDITWLEEPVRWHDQPLGLALVAHQTRIPI
                     AGGEGESTLFGCRDILERGGLTYLQADILGGGGYTNLRKIAGLAQAYHVKFAPHGASF
                     PELVAPLVAALPNGDSVPATTPHQPPAVWGDLYEEFVITRGRIHLTTKAGLGLAFNQE
                     FLTRYQVARLD"
     gene            571..1875
                     /locus_tag="VFU72_13195"
     CDS             571..1875
                     /locus_tag="VFU72_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424495.1"
                     /translation="MRETATAARVIYAGTLIDGTGAPPVRDAAVVIADGAITYAGPAA
                     GAPDRTAIPAAQRLDAPDGTVLPGLIESHVHLTFNTRTAANSPAVIAQLVTDDDWMIA
                     IRAVQAAQACLGAGITTVRDCGAKGLVTMRLRDLIAAGFINGPRVLACGMPITTTAGH
                     CHWCGLEANNEQEVILATRRMAREGADFIKVMATGGGMTVHSNITEPQYSVAELTAIR
                     KESHRLGRRVTAHSHAGKGQRNCLDAGIDMIEHCNWHTPDGWEFDEALMHDIVAAGMY
                     VGITLSGPQQAAAAAGTPYSELPESLRVRYETLHEMRAMGTKIVLHSDAIAPITTYED
                     FPFSLIAAVRYGGFSPVEAIHASTGLAAEAIGLAGVTGTLTSGQAADVLVVDGDLAAD
                     IQAITRTRQVLRDGRVVATAGWVRATPSAMPGHELRRVAQGE"
     gene            1929..4136
                     /locus_tag="VFU72_13200"
     CDS             1929..4136
                     /locus_tag="VFU72_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424496.1"
                     /translation="MRQNVAVLYSEATRQTYIQEAREQDELYAFTGADGQQRHWLKGQ
                     YAYLAPNAATALLDRYNIKHDTISEAEVAAGVLSAYRALFVPNGGALLEATRAALQTW
                     LTAAGDDAAGRRLVVTGRTNLPPALLGLAECAPITPVGYTGWRFVDGPFADNERWEEF
                     YLTSYRGYTASRATAVPGARVLADLYEFTGDTTSAATAASRRLGAAIVTTGQTLYIAN
                     QVLEYLGGAFQAQIDVDRLRDWYNPVYYLDTLGYLMCELLRNLGLRDLFATQLRSFGT
                     YAGALVLRHDADTGPSEALDLAMLEWEIANQVPATYVVLDPAVSGEHTTPLASKTWVA
                     QAERSNLLEVGLHNDGYAGSPPTYVAGRAMFEHFRDGDRALGITSRTAGRHYGFHRHP
                     ESLDAMEYLYDRAPALLGLCTFSVLQVIAYGVADPAVTWLGRSITYSTRFRAGAWTSG
                     AVCGWWFPFHVVIATAEYRRVLRGWDSTKESDCDYDRADALLAGQNSKDPRTPSRLPN
                     GVYTVQYHPQHARDAALNGGRGSLPWVRYLGALAERRNLWLANKRMVYARLNDYQDLL
                     FRIEADGAIAVANPTGRDIMGLMIRLPGPVSAVLAGDDVCVHVVPLADEDGGVACTLP
                     PLAPGAAVRLRAVAEQPDTPVIAQPNSKTFEIVSALYRPSRGELTVTARAVAHSALVA
                     ERLRPGQWYAVTLAGPGSAPDRLPAAADGRLTIPLEAPRETVVTLEITVREAL"
     gene            4133..4894
                     /locus_tag="VFU72_13205"
     CDS             4133..4894
                     /locus_tag="VFU72_13205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU5424497.1"
                     /translation="MSALFDLTGRVAVVTGGGKGIGRELALALAGAGVDIVVASRTEA
                     ETRAVAAEIQARGRRALPHHLDVTVGASISALMDDTLRAFERLDVLVNCAGRVGLRPS
                     FELSEQEWDDIVATNLKGLFFCCQAAGRHRAARGGGKIINIASALGLVGLDQRAAYCA
                     SKGGVIQLTRALAVEWAPQGITVNAIAPTTTLTGETAHLYADPAVQAAKARDIPLGRL
                     GVARDLLGAAIYLASPAADFVTGHTLVVDGGYSVH"
     gene            complement(5731..5979)
                     /locus_tag="VFU72_13210"
                     /pseudo
     CDS             complement(5731..5979)
                     /locus_tag="VFU72_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6472..7179
                     /locus_tag="VFU72_13215"
     CDS             6472..7179
                     /locus_tag="VFU72_13215"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU5424498.1"
                     /translation="MATANPLWGAERIRGELLKLDIRVAKSTIQRYRREAHPPRRTGQ
                     TWATFLQNHAAAIWACDVLPVTDLLFRPLFAFFVIALETRRVVHVGVTRHPTDAWVAQ
                     QLREAPPFDQRLTYLLRDNDSKYGPTFARVAAATGIAELRTAYRAPRQNATCERFLGS
                     VRRECLDHVLDFGEGRLRRVLGEYVRYFNRDRPHQGLAQRIPEAEGTGASRGEQRGAV
                     RAIPILGGLHHAYARAA"
     gene            complement(7161..7454)
                     /locus_tag="VFU72_13220"
     CDS             complement(7161..7454)
                     /locus_tag="VFU72_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424499.1"
                     /translation="MAQHEGTSRPRVLVVPGPRLYGELFTPEADAALRTLADVTLNPR
                     DENWTAATLAAALQGVDAVITGWGTPSFDDEVLAACDLSWPRSPSMRRHAARA"
     gene            complement(7487..8260)
                     /locus_tag="VFU72_13225"
     CDS             complement(7487..8260)
                     /locus_tag="VFU72_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424500.1"
                     /translation="MLDASAWVGTWAFAPSQRVALAGLVRTLTVAGLAGAAVSPVEAV
                     LAPEPMAANRHLLAAAARRRAGDFAIVPVPILDPSLPAWREHLAECSAQRGDVLRAVK
                     IVPNYHEYSLGAPAVAALAAELARRDLGLCVQLRLADERAHRPSLVVPGVDPACVTAL
                     AVAQPDLRVLVCGAYMSDLAVLAPAANIATELSFVESGYLLRDALAHLGPDRLLLGTH
                     APLHYAAPGVAKLGADALSPGVSAQLGTDNFRRFFGMMT"
     gene            complement(8253..9047)
                     /locus_tag="VFU72_13230"
     CDS             complement(8253..9047)
                     /locus_tag="VFU72_13230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424501.1"
                     /translation="MIVDIHIHLHSAGCRYDQHEVAECMRLARRAGIERAIYLFNLAD
                     TGGYDPAPGDVRRSNDLGLRLVTEHPDFFTGFCYLNPAHDPGFIEEELARCVADGPLQ
                     GIKLWVAVKATDPRLDPILTRAVELGVPVLYHAWYKATGYAYNESTPAEIADLARRHP
                     RATIIMAHLGGGGWRGVQDIKSCPNVVVDTSGAQPQAGLVEYAVRELGAERVVYGSDW
                     PIRDFATQVARVTGARLTAMDRASILGGNAARLVPGLAAKEGCADA"
     gene            complement(9071..>10165)
                     /locus_tag="VFU72_13235"
     CDS             complement(9071..>10165)
                     /locus_tag="VFU72_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5424502.1"
                     /translation="LGPIALGYKNERVDAAIHTQLDRGIIYGLLAPLEVEVARLLTEL
                     VPCAERVRFFKGGGEATAAAARVVRRYTGREVILNCGYRGWPDVWSVTTGDPGVPRGL
                     AGSVETFPFNDLEALERAFIAHQGEVAAVFLDVAATPPDMSYLGDVRDLAHEQGALLV
                     FDEIVTGFRLAIGGAQEYFGVTPDLACFAKALANGMPLAAVVGRADVMDAFTDAAISL
                     TYGGEALSLAAAKATLETYRDEPVIAALWARGQQLRTGLEAAARAAGVPFATRGYDPM
                     TGMTFLGLDPDADQHAWTFVLQELAAQGVLLRRRGLNFVTYSHRETDIAQAVDAAHTV
                     FAALAPLLGTPELVRRLHTTRVAEGFRRFA"
BASE COUNT         1673 a         3449 c         3419 g         1624 t
ORIGIN      
        1 cgacccgcca acggccatcg ccgtggcccg gcagctcgaa gcgtacgata tcacctggct
       61 cgaagaaccg gtgcgctggc acgaccagcc attgggcctc gccctggtcg cgcaccaaac
      121 ccgcattccg atcgccggcg gcgaaggcga aagcacgtta ttcggctgcc gggacatcct
      181 ggagcgaggc ggcctcacgt acttgcaggc cgatattctc ggcggcggtg gctataccaa
      241 tctccgtaag atcgcggggc tggcgcaagc ctatcatgtg aagtttgcgc cgcatggcgc
      301 gagttttccg gagctggtcg cgccgctggt cgcggccctc ccgaatggtg actccgtgcc
      361 ggcgacgaca ccacaccagc caccggcggt ctggggcgat ctgtacgaag agttcgtgat
      421 cacccgtggc cggatacacc tgacgacgaa ggctggcctg ggcctggcat tcaaccagga
      481 atttctgacg cgctaccagg tcgctcgcct ggactgaggt cgttagtcgg caggaataca
      541 ttgcctgcct taccatgagg gggaagactt atgcgcgaga ctgccaccgc ggcgcgcgtc
      601 atttacgccg ggacactgat cgacggcact ggtgcgcccc cagtgcgcga cgcagccgtc
      661 gtcattgccg atggcgcgat cacgtatgct ggccccgccg ctggcgcgcc ggatcgcacg
      721 gcgataccgg cggcgcagcg tctcgacgcc ccggacggca cagtgctgcc gggcctgatc
      781 gagagccacg tccacctgac cttcaatacg cgcacggcgg cgaactcgcc agcggtgatc
      841 gcgcaactgg tgaccgatga tgactggatg attgccattc gcgcggtgca ggccgcgcag
      901 gcgtgtctcg gtgctgggat caccaccgtg cgcgactgcg gcgcgaaagg gctggtgacg
      961 atgcggttgc gcgacctcat cgccgccggc ttcattaacg ggccacgcgt gcttgcctgc
     1021 ggcatgccga tcaccaccac ggccggtcat tgccactggt gcggcctgga ggccaacaac
     1081 gagcaggagg tcatcctcgc gacgcgccgc atggcgcgtg aaggtgccga cttcatcaaa
     1141 gtcatggcaa ccggaggcgg catgaccgtc cacagcaata ttaccgaacc ccagtactca
     1201 gtggctgagc tgaccgccat caggaaggag tcgcaccgcc tcggccggcg cgtcaccgcg
     1261 cactcgcacg ccgggaaggg gcagcgcaac tgtctcgacg cggggatcga tatgattgag
     1321 cactgcaact ggcacacacc tgacggctgg gaatttgacg aggcgctgat gcatgacatc
     1381 gtcgcggccg gaatgtatgt cgggatcacg ctttcggggc cgcagcaggc agccgccgca
     1441 gcgggcacac cctacagcga actaccagag agcctgcggg tgcgctacga gacgttgcac
     1501 gaaatgcgcg cgatgggcac caagatcgtg ctgcacagcg acgcaatcgc gccgatcacc
     1561 acctacgagg actttccgtt cagcctgatc gccgccgtgc gctacggcgg cttctccccg
     1621 gtcgaggcga ttcatgccag caccggcctg gcggcggagg cgatcggtct cgcgggcgtc
     1681 actggcacgc tgacctccgg ccaggcggcc gatgtgctgg tcgttgacgg cgatctcgcc
     1741 gccgatatcc aggcgatcac gcggacgcgc caggtgttgc gcgatggccg tgtcgtcgct
     1801 accgctggct gggtgcgcgc gacgccctcc gcaatgccgg gacacgaact acgtcgcgtg
     1861 gcgcagggtg agtagcgcca gacgcgcagc cctcgcgctc ctcgccgttg caggaaacga
     1921 ggtgaacgat gcggcagaac gtcgcggtgt tatatagcga ggcaacccgc cagacctaca
     1981 tccaggaggc gcgggagcag gacgaactct acgcctttac cggtgctgat ggccagcagc
     2041 gtcactggct aaaggggcaa tacgcctacc tggcgccgaa cgccgcgacg gcgctgctcg
     2101 accgctacaa catcaagcac gatacgatct ccgaagccga agtcgcagcc ggcgtgctca
     2161 gtgcctatcg cgcgctcttc gtgccgaacg gtggcgcgtt gctagaggca acgcgcgcgg
     2221 cgctccagac ctggctcacc gctgcgggag acgatgctgc cggacggcgt ctcgtcgtaa
     2281 ccggacgcac caatcttccc cccgcgctcc tcggtctggc ggaatgtgcg ccgatcacac
     2341 cggtgggcta cactggctgg cgtttcgttg acggtccgtt tgccgataac gagcgctggg
     2401 aggaattcta cctgaccagt tatcgtggct acaccgcctc gcgagcgacg gcggtgccgg
     2461 gcgctcgcgt actcgccgat ctctacgagt tcaccggcga caccaccagc gccgccacgg
     2521 cggccagccg ccgactgggt gctgcgatcg tcacgaccgg gcagacgctc tacatcgcca
     2581 accaggtgct cgaatatctg gggggagcgt tccaggcgca gatcgatgtt gatcggctgc
     2641 gcgactggta caacccggtc tactatcttg ataccctcgg ctacctcatg tgcgagctgc
     2701 tccgcaacct cggactgcgc gacctgttcg cgacgcagct caggagcttc gggacttacg
     2761 ctggcgcgct cgtcctgcgc cacgacgccg ataccggccc cagtgaggcg ctggacctcg
     2821 cgatgctgga gtgggaaatc gccaatcagg tgcccgcgac ctacgtcgtc ctcgaccccg
     2881 ctgtatccgg cgagcacacc acgccgctgg cgagtaagac ctgggtcgcc caggccgagc
     2941 gcagtaatct cctcgaagtc gggctgcata acgacggtta tgcgggatcg ccaccgacct
     3001 acgttgccgg ccgcgcgatg ttcgagcatt tccgggacgg cgaccgtgcg ctcggcatca
     3061 ccagccggac cgccgggcga cactacggtt tccatcgtca tccggagtca ctcgatgcga
     3121 tggaatatct ctacgaccgc gcgccggcgc tgctcggcct ctgcaccttc agcgttttgc
     3181 aggtgatcgc gtacggggta gcagatcccg ctgtcacctg gctgggccga tcgatcacct
     3241 actcgacgcg gttccgcgcc ggcgcctgga ccagcggcgc ggtgtgcggc tggtggttcc
     3301 ccttccacgt cgtcatcgcc accgctgagt atcgccgggt gctgcgcggc tgggattcaa
     3361 cgaaggagag tgactgcgac tacgaccgcg ccgacgcgct gctggccggc cagaatagca
     3421 aggatccgcg cacgccgtcg cgcctgccga atggtgtgta tacggtccag tatcatcccc
     3481 agcacgcacg cgacgcggcg ctgaacggcg gccgaggctc tttgccctgg gtgcgctacc
     3541 tcggcgcgct ggccgagcgg cgcaatctgt ggctggcgaa caagcgcatg gtctacgcgc
     3601 gcctgaacga ctatcaagac ctactctttc ggattgaagc ggacggcgcg atcgccgtcg
     3661 ctaacccaac tggccgcgac atcatggggc tgatgatacg gctccccggt ccggtcagcg
     3721 cggtgctggc tggcgacgat gtctgcgtcc acgtcgtccc actcgctgac gaggacggcg
     3781 gcgtcgcctg caccctgccg ccgctcgcgc caggcgcagc cgtgcggctg cgcgccgtcg
     3841 cggagcagcc tgataccccg gtcatcgcgc agccgaatag caagacgttt gagattgtca
     3901 gcgccctcta ccgtccgtcg cggggcgagc tcaccgtgac cgcgcgggcg gtcgcccaca
     3961 gcgcgttggt agcggagcgc ctcaggccgg gccagtggta tgctgtcacc ctcgccggcc
     4021 caggcagcgc gcctgatcgt ctgccggctg ctgcggacgg ccggctgacg atcccgctgg
     4081 aagcgccgcg cgagacggtt gtcacgctgg agatcaccgt gcgcgaggca ttgtgagcgc
     4141 tctcttcgac ctgaccggcc gggtcgccgt cgtcaccggc ggtggcaagg ggatcggccg
     4201 cgaactggca ctcgccctcg ctggcgctgg cgtggacatc gtcgtggcga gtcgaactga
     4261 agctgaaacg cgcgcggtcg cggccgagat ccaggcccgt ggccgccggg cgctgccgca
     4321 tcacctggat gtcaccgtgg gcgcgagcat cagcgcgctg atggacgaca cgctccgcgc
     4381 gttcgaacgg cttgacgtgc tggtcaactg tgctggccgc gtcggcttgc gcccttcgtt
     4441 cgaactgagc gagcaggaat gggacgacat cgtcgcgacc aacttgaagg gactcttctt
     4501 ctgctgccag gcagcagggc ggcacagggc cgcgcgcggc ggggggaaga tcatcaacat
     4561 cgcctcggcg ctcggcctgg tcggactcga ccagcgcgcg gcctattgtg ccagcaaggg
     4621 tggggtgatc cagctcaccc gcgcgcttgc cgtggagtgg gcgccgcagg gcatcaccgt
     4681 caacgcgatc gcgccgacga ccacgctcac tggagagacg gcccacctgt acgctgaccc
     4741 ggcggtccag gcagcgaagg cgcgcgatat cccgcttggt cgtctcggtg tggcgcggga
     4801 tctcctggga gcggcgatct atctcgcgag ccccgccgcg gacttcgtca ccggtcacac
     4861 cctggtggtc gatggtggct acagtgtcca ctgagcgtcg tccatggatt ctggcgccct
     4921 gtgttggctc cgcggcccat cctgcgctgt ccatggtccc gcaaggggca tgggtctgca
     4981 tcaggatgcc agccgaaccc ggcgaccagg acggtgagag taacgcgaga gcgtgatgag
     5041 agcgagcgtg ccctggtgac cacatcgatg tggcctgggc agcgcggatt gtcggcgcgg
     5101 cgagtccggc gggtgtaccc gcccagcgac gctaggcaca agaaaaggcc ctgctggtag
     5161 ggtgcgaata cttcctccgc agcccgattc tggcagacgc ttggccgagg tgccaaaatc
     5221 cagttgctag tggcgaacag ggctggcacg ggcattgggt agaatacaca tcgtgagcgg
     5281 atccagagta cactgcgagt acatcgcaga gcatcttgag accacggtgg ggatcaggcg
     5341 cggacatcgt gcagttgctc cggtcggcgg cgcagcgatc cgataggatt aggagtacgg
     5401 ccggtctgtg ctcgctgtca ccaggcgagc gtggcgcagc ggcctaaggg gattggcggc
     5461 gatttccctt ggtagcagtg tgctggctac ttggctcatc caggcacgat tgctagcagt
     5521 ctcgtacgag gagtgccagg attacacgcc ggatgagcaa agtagatacg gtattgcttg
     5581 tcaagccctc tggtcagcga acactggcgg ccccggtcaa gcggcgaggc cgggcgaggc
     5641 gtcgtggtgt tgttgatccg ggttgaaatg ctggagtttg ctgccgagcg cccaggcctg
     5701 atgcaggcgc tttcgggcag cggcgcagcg cgcgacctgc agcggcttgc ccgccgcgcg
     5761 caggggccgg tagcaggcct tgatcgcggg attgtgctgc gcggcgctga gggtggccag
     5821 ataaagcgcg gtgcgccggc gggcgttgcc gtcgtggccg atcgccggcc gggcacggac
     5881 gctgcggccc gactcgcgcg gctgtcgtgg ccgaaaagtc catccgtgcc gccaggccga
     5941 ttccttggct gcccgacgcg cccttctctg ccacgcgata acccctgcgt gagcttgcgg
     6001 ctacactctg cttcgttcga tgtcgcgccg cacacgaagg aggcgctacc catgcctggc
     6061 cgtctgctgc gccgcctgat cgccgtcggc cgcgccgctg cgcaggcatt ccaccggcga
     6121 ctgctggctg ccgcccgccc ggcagccgcg cccctcgttg ctggcaccct cgccgatctg
     6181 gcgcggagta agccagcgct ggtggccgag aacgcgctcc tccgccacca gctcgctatc
     6241 ctgcggcgcg gcgtcaagcg cccgcgctgc cgcccggccg accgtgcgct gctggtgctc
     6301 ctggccagcc gcgtccgcgc gtggcggtcg gcactcctca tcgtgcagcc cgccacgctg
     6361 ctgcgctggc atcgccaact gtttcgctgg tcctggcacc ggcgctcccg ggctgccgcc
     6421 ccggcgcacc gtccgcccct tgcgcccgag acggtcgccc tgattcgcga gatggcgacg
     6481 gccaatccct tgtggggtgc cgagcgcatc cgcggggagc tgctcaagct cgacatccgc
     6541 gtcgccaagt cgacgatcca gcgctaccgg cgtgaggccc acccgccgcg ccgcaccggc
     6601 caaacctggg cgaccttcct ccagaaccat gccgcggcga tctgggcctg cgacgtcctg
     6661 ccggtgaccg acctgctctt ccggccgctg tttgccttct tcgtcatcgc gctcgagacg
     6721 cgccgcgtgg tccacgtcgg ggtgacgcgc catcccaccg acgcctgggt cgcccagcaa
     6781 ctgcgcgagg ccccgccctt cgaccagcgg ctgacctacc tgctccgcga taacgacagc
     6841 aagtatgggc cgaccttcgc acgcgtcgcc gcggccacgg gcatcgcgga gctgcgcacc
     6901 gcctaccgcg caccccggca gaacgcgacc tgcgagcggt tcctcgggag tgtccgccgc
     6961 gaatgcctgg accatgtgct cgacttcggc gaagggcgcc tgcggcgggt cctcggcgag
     7021 tacgtgcgct acttcaatcg cgaccggccg caccagggcc tggcccagcg catccccgag
     7081 gcagagggga ccggggcgtc gcgcggcgag cagcgcgggg ctgtgcgggc gatcccgatc
     7141 ctgggtggcc tccaccacgc ctatgcgcga gccgcatgac gtcgcatcga tggacttctc
     7201 ggccaggaca ggtcgcaagc ggcgagcact tcgtcgtcga atgacggcgt gccccacccg
     7261 gtgatcacgg catcgacgcc ctgcagcgcc gccgccagcg tggcggcggt ccagttctca
     7321 tcgcgaggat tgagcgtcac gtctgccagc gtacgcagtg ccgcgtcagc ctcaggggta
     7381 aacaactcgc catagagccg cggacccggt accaccagga cccgtgggcg gctcgttccc
     7441 tcgtgctggg ccattttcct cctcgctctt cgccactagg tggacgctac gtcatcatgc
     7501 cgaagaagcg ccgaaagttg tcggtgccaa gctgcgcgct cacgccgggc gacagcgcat
     7561 cggcacccag cttcgccacg cctggcgcag catagtgaag tggggcgtgt gtaccgagca
     7621 agagacggtc aggcccgaga tgcgcgagag cgtcacgcag caagtagcct gactcgacga
     7681 acgatagctc ggtggcgata ttcgccgccg gtgccaggac agcgaggtcg ctcatgtacg
     7741 cgccgcagac gaggacgcgg aggtcaggct gcgcgactgc cagggccgtg acacaggccg
     7801 gatctactcc cgggactacc agtgatgggc ggtgggcgcg ctcatccgcc agccgcaatt
     7861 gcacgcagag gcccagatcg cgccgcgcca gttccgcggc cagcgcggcc accgcgggcg
     7921 cgccgagcga gtattcgtgg tagttcggca cgatcttcac cgcgcgcagt acgtcaccgc
     7981 gctgcgcgct gcactcagcg aggtgctcac gccaggctgg caaggaggga tcgaggatgg
     8041 gcaccgggac gatcgcgaaa tcgcccgctc gccgccgcgc cgccgcggca agcagatggc
     8101 ggttcgcggc catgggctct ggcgcgagca cggcctctac cggcgacacg gcggcaccgg
     8161 caagtccagc gaccgtcaat gtgcgcacga ggccagcgag cgctacgcgc tgactggggg
     8221 cgaaggccca ggtgccaacc caggcgctgg cgtcaagcat cggcgcaacc ctcctttgcg
     8281 gccaggccgg gtaccaggcg agcggcattg cccccgagga tggacgcccg gtccatggcc
     8341 gtgaggcgcg caccggtgac acgcgccacc tgcgtggcga agtcacggat cggccaatcc
     8401 gatccataga cgacccgctc agccccgagt tcccggacgg cgtactcaac gaggccagcc
     8461 tgtggttgcg caccggaggt gtctacgacg acgttgggac aggacttgat gtcctgcacg
     8521 ccccgccagc caccgccgcc gaggtgcgcc atgatgatcg tcgcgcgtgg gtggcggcgg
     8581 gcaaggtcag cgatctccgc cggcgttgat tcgttgtagg cgtagccggt cgccttgtac
     8641 caggcatggt agaggaccgg gacgccgagt tcgaccgcgc gcgtcaggat tgggtcgagg
     8701 cgcgggtcag tcgctttgac cgccacccag agtttgatgc cctgtagcgg cccgtcagcc
     8761 acgcagcgcg cgagctcctc ctcgatgaag ccgggatcgt gcgcgggatt gaggtagcaa
     8821 aagcctgtga aaaagtccgg atgctcggtg acgagccgca ggccgagatc attgctccgg
     8881 cgcacatctc caggggccgg gtcatagccg ccagtgtcgg cgaggttgaa gaggtagatg
     8941 gcacgctcga tacccgcccg gcgcgcgagg cgcatgcact cagcgacctc atgctggtcg
     9001 tagcggcagc cagcgctgtg caaatggata tggatatcaa cgatcatcaa tcccgttcct
     9061 cttctggtct tcaggcgaag cgccgaaagc cctcggcaac tctcgtcgta tgcagccgcc
     9121 ggaccagttc cggcgtgcca aggagtggcg cgagcgcagc aaaaacggtg tgcgcggcgt
     9181 ccaccgcttg ggcgatgtct gtctcacggt ggctgtaggt tacgaagttg aggccgcggc
     9241 ggcgcaggag gacgccctgc gccgccagct cttgcagaac gaaagtccag gcgtgctggt
     9301 cggcgtccgg gtcgaggccg agaaaggtca tccccgtcat cggatcgtag ccgcgtgtgg
     9361 cgaaaggaac accggccgcg cgagccgccg cttccaatcc tgtacgtagc tgctggccac
     9421 gcgcccagag ggccgcaatc accggctcat cgcgataggt ttccagcgtc gccttggccg
     9481 cagccagcga cagcgcctca ccgccgtaag tcagcgagat cgcagcatcg gtgaaggcgt
     9541 ccatgacgtc cgctcgccca acgaccgccg ccagcggcat gccgttcgcc agagccttcg
     9601 cgaagcacgc caggtcaggc gtaacgccga agtactcctg tgcgccaccg atcgccagcc
     9661 ggaagcccgt cacgatttca tcgaaaacga gcagcgcgcc ctgctcatgt gccaggtcac
     9721 gtacgtcgcc caggtagctc atgtcgggcg gcgtggcggc cacgtcgagg aagacggccg
     9781 cgacctcgcc ttggtgagcg atgaacgcgc gctccagtgc ttccaggtcg ttgaagggga
     9841 aggtctcgac cgacccggcc aacccacgcg gcacacccgg gtcgccggtc gtcacactcc
     9901 agacgtcggg ccagccccgg taaccacaat tgagaatgac ctcgcggcca gtgtagcgcc
     9961 gcacgacgcg tgcagcggcc gcggtcgcct caccaccgcc cttgaagaac cgaacgcgct
    10021 cggcacacgg taccaactcg gtgaggagcc gcgccacctc gacttcgagc ggcgcgagga
    10081 ggccatagat gatgccccgg tcgagttgcg tgtgaatggc agcgtcaacc cgctcgttct
    10141 tatagccgag cgcgattggc ccaag
//
LOCUS       DASUBI010000683         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_373085, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000683 DASUBI010000000
VERSION     DASUBI010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373085"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFU72_13240"
     CDS             <1..533
                     /locus_tag="VFU72_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424503.1"
                     /translation="GYAIQAALRLRGEETSGRAEPLLGTATGRIGWAASHLVFALLGP
                     AAALTAAGLAAGLVYGLEAGDASRQVPRVLAGALVQLPAVWVLAAVAVVLFGRLPRFS
                     AVSWAVLAGCLFLLLVGAAIQLDRRIMDLSPFSHIPHLPGGALSVVPLAGLTLLAALL
                     IVAGLAGLRRRDIPAA"
     gene            708..1592
                     /locus_tag="VFU72_13245"
     CDS             708..1592
                     /locus_tag="VFU72_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5424504.1"
                     /translation="MELIGYLGALVLAAVLALGAQYLFTRSLTVGKELISLFVLGFIG
                     VYAGSELFGEFGDKISSLSAQGPSFGGFYVLTGLVGGLIMVAATAFGFLRQQRGLATD
                     MKSQGIADPKIAHIMFNDSRVAALWLGIRLYVGYEWLAAGYHKWLGPGWMDGGSALKG
                     FWTSAVAIPQTGSPRITYGWYREFLTYMLNHGWYEWFAKLITVGEMLVGIGLIVGALV
                     GIAAFFGALMNFSFMLAGSASTNPVLLIFSVGLILAWKVAGYWGIDRYLLPELGAPWS
                     PGKLFEQEEPRATAVAGD"
     gene            complement(1910..>2300)
                     /locus_tag="VFU72_13250"
     CDS             complement(1910..>2300)
                     /locus_tag="VFU72_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5424505.1"
                     /translation="EGIEMGTRFVATQECIAHDNYKEALVAAKESDTTIIERSLGRPG
                     RALAGEAAERVLALEREDAPREEMLAAISGRVNRLGALEGRLEEGWVWSGQVAGLIHD
                     VPTVQELLDRMVREAEEHVRQALAAFG"
BASE COUNT          306 a          804 c          772 g          418 t
ORIGIN      
        1 cgggctacgc cattcaggcg gcgctgcgcc tgcgcggcga ggagaccagc gggcgcgccg
       61 agccgctgct cggcaccgcc accggccgca tcggctgggc cgccagccac cttgtcttcg
      121 cgctgctcgg tcccgccgcc gcgttgacgg cggcggggct tgccgcgggc ctggtctacg
      181 ggctggaggc gggcgacgcc agccgccagg tgccgcgggt gctggccggc gcgctggtgc
      241 agttgcccgc cgtttgggtg ctggccgcgg tcgcggtcgt cctgtttggc cggctgccgc
      301 gcttcagcgc cgtcagttgg gccgtgctgg ccggctgcct cttcctgctg ctggtcggcg
      361 ccgccatcca gctcgaccgg cgcatcatgg acctctcgcc cttcagccat atcccgcatc
      421 tgcccggcgg ggcgctgagc gttgtcccgc tggccgggct gacgctgctg gccgcgctgc
      481 tgatcgtggc cgggctcgcc ggcctccgcc gtcgcgacat accagccgcc tgagcgcgcc
      541 ggctcctcgc tccgaactcc gctcatcatc gtctgtgaag actgtcacga tcctgtaaca
      601 ccacccgcgt tgccgaaaca ttcgtgacat ggggtggcgg ctatcctaca gccatgaaat
      661 gagagcggag tggccccaaa ggccacgtgg cggaaggaag gctcgtcatg gaactcatcg
      721 ggtatctcgg ggcgctggtt ctggccgcgg tgctggcact gggcgcacag tatctcttca
      781 ctcgcagcct cacggtgggc aaggagttga tctcgctctt cgtgctcggc ttcatcggcg
      841 tctacgccgg cagcgagctg ttcggcgaat tcggtgacaa gatctcctcc ctcagcgcgc
      901 agggtccgtc gttcggcggc ttctacgtac tgacgggtct ggttggcggt ctgatcatgg
      961 tggcggccac cgccttcggc ttcctccgcc agcagcgggg tctggccacc gatatgaaga
     1021 gccaggggat cgccgatccc aagatcgcgc acatcatgtt caacgatagc cgggtcgcgg
     1081 cgctctggct gggcattcgc ctctacgtcg gctacgagtg gctggcggcc ggctaccaca
     1141 agtggctcgg accgggctgg atggacggtg gctcggcgct caaggggttc tggacctccg
     1201 ccgtggccat cccgcagacc gggagcccgc gcatcacgta cggctggtat cgggaattcc
     1261 tgacctacat gctgaaccac ggctggtacg agtggttcgc caagctgatc acggtcggtg
     1321 agatgctggt cggtatcggg ctgatcgtcg gcgcgctggt cgggatcgcg gccttcttcg
     1381 gggcgctgat gaacttcagc ttcatgctgg cgggctcggc ctcgaccaac ccggtgctgc
     1441 tgatcttctc ggtcggcctg atcctggcct ggaaggtcgc gggctactgg ggcatcgatc
     1501 gctacctgct gccggagctg ggcgcgccct ggtcgccggg caagctcttc gagcaggaag
     1561 agccacgggc gacggcagtc gccggagact aaacaggaca acacgaccca cgctctcatg
     1621 ccgaagagcc ctggccattg gccagggctc ttcgcattcc cgccgagcgg ccgagaaaac
     1681 gatggtggct gcgagcgctg cctagcgcac gacgtagagt gtcttggcca ggcggggcct
     1741 tccttctgag cacgtcggtc agcgtgtgga gacggatcct tcgacaagct caggatgaca
     1801 gggggaggga ggtggccttc cggatggcac agttcgtcgt cgtcagcccc ccattaaccc
     1861 tgtcgtcccg agcgcagtcg agggatccgt gccgcacgcg ccccgatctc tatccgaagg
     1921 ccgccagcgc ctgccgcacg tgctcctcgg cctcgcgcac catgcggtcg agcagctcct
     1981 ggacggtcgg cacgtcgtgg atcaggccgg ccacctgccc cgaccagacc cagccctcct
     2041 ccagccgccc ctccagcgcg ccgagcctat tgacccgccc gctgatggcc gccagcatct
     2101 cctctcgcgg cgcgtcctcg cgctccagcg ccagcacgcg ctcggctgct tccccggcca
     2161 gcgcccgacc cggcctcccc aggctgcgct cgatgatggt ggtgtcggac tctttcgccg
     2221 ccaccagcgc ctccttgtag ttgtcgtggg cgatgcattc ctgggtggcc acgaagcgcg
     2281 tgcccatctc gatcccctcg
//
LOCUS       DASUBI010000684         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_711116, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000684 DASUBI010000000
VERSION     DASUBI010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711116"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..389)
                     /locus_tag="VFU72_13255"
     CDS             complement(57..389)
                     /locus_tag="VFU72_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEU5424506.1"
                     /translation="MDVQQRLTQAQDAMSVRRVYGEPFEKDGATVIPAARIRGGGGGG
                     QGRQPNQEQEGSGYGFGLVADPAGAFVIKEGKVTWVPALDVNRVILGGQIIAVVGLLV
                     LRSILGKK"
     gene            544..942
                     /locus_tag="VFU72_13260"
     CDS             544..942
                     /locus_tag="VFU72_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEU5424507.1"
                     /translation="MRLRYHPDDNSLRLTRDAGGDVPTHQLGLPGLVDVGEGGRLVGV
                     ELHAAPGLDLARALAPWRGDRVAAEYVSVDDATAYIELSAPEEASLREQTRAAAALLA
                     VELDESGALVALSIPRRGAGYEISYPSGNQ"
     gene            1152..2138
                     /locus_tag="VFU72_13265"
     CDS             1152..2138
                     /locus_tag="VFU72_13265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424508.1"
                     /translation="MTVIDVHTHILSEEYIRLLAEHGGPTYEVKVDRSGGPTIHRNGA
                     PFHTLQPAMFDVDMRLQAMDRAGVDIGVISLAAPSVYWGSDADSLQAARQMNDYIARI
                     CREHPDRFAGIATLPWRSAADSLPELDRAIDQLGHKGVMVLANIEGRPLTDPTYAPIW
                     EAIDRRGLAVFVHPITPPGADQMDMLDYNLVASVGFMIDTTLAFARMIYDGFFDRYPN
                     ISMIAPHAGATLPYLAGRLDQCWDKMPACRVNISEPPSSYLRRIYYDTVAYAQNAIDL
                     CLAVGGPSQLLYGSDYPHNISDMAGCLARAEALPGDVVEDVKSRNAMRIFGL"
     gene            complement(2255..>2519)
                     /locus_tag="VFU72_13270"
     CDS             complement(2255..>2519)
                     /locus_tag="VFU72_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424509.1"
                     /translation="QELDAFVGTLAGLFEQAAAHGEIRDISPRLGAFLFFHTLVAAVM
                     PSLSDSLVQIPSDPAAAARLITSIVLHGIASKDQEHGADGAPG"
BASE COUNT          389 a          867 c          880 g          383 t
ORIGIN      
        1 ggcattggga gagtcttcgt gtgggccggg ggtgggggtg ggggtgaggg cgtccgctac
       61 ttcttgccca ggatggagcg caggaccagc aggccgacga cggcgatgat ctgcccgccg
      121 aggatgaccc ggttgacgtc cagcgcgggt acccaggtga ccttgccctc cttgatcacg
      181 aaggcgccgg ccgggtcggc gaccagcccg aagccgtagc cggagccttc ctgctcctgg
      241 ttgggctggc ggccctggcc gccgccgccg cctccccgta tccgggccgc cgggatgacg
      301 gtcgcgccat ccttctcgaa cggctcgccg tagactcgcc gcacgctcat ggcgtcttgc
      361 gcctgcgtca gtcgctgttg cacgtccacg ctgttcctcc gtgatcatga tgccgagctg
      421 gtccgttgcc ctgagcgcgc acgattctcg ccacgggcgt ggggccggat ggtggatcgc
      481 aatcggcggt atgatcggtg aggatccggc cgggggtgcc ggaggtacga gagcgaatgg
      541 gccatgcggc tacgctacca ccctgacgac aacagcctac ggctgacacg cgacgccgga
      601 ggcgacgtgc ccacgcacca gctgggcctg cccgggctgg tcgatgtcgg cgagggcggg
      661 cggctggtcg gcgtcgagct gcacgcggcg ccggggctcg acctggcgcg ggcgctggcg
      721 ccctggcgtg gcgatcgggt cgccgccgaa tacgtcagcg tagacgacgc caccgcctac
      781 atcgaattga gcgcgcccga ggaagcctcg ctgcgcgagc agacgcgggc cgccgcggct
      841 ctgctggcgg tcgagctgga cgagtcgggc gccctggtgg cgctctcgat tccgcggcgc
      901 ggcgcgggct acgagatctc ctaccccagc ggcaatcaat gatggcaccc cgagcggccc
      961 ggcgggccgc gcgcctgggt ctgcgaggtc gactagccgc cggcttcccg gaaagagcta
     1021 tccgctcacc ccttgacggg cgaccaaagt tccttagact cggatcatcg caataacgac
     1081 gcatcactgg ttgcgcgctg tgaccggcgc cgcgaccgcg acgcgtgatc gcgcaaggag
     1141 cggagggcga catgaccgtg atcgatgtcc acacccacat cctgagcgag gagtacatcc
     1201 gactgctggc ggagcacggc ggccccacct acgaggtcaa ggtcgaccgc tcgggcgggc
     1261 cgacgatcca ccgcaacggc gcgccctttc acacgctgca gcccgccatg ttcgacgtcg
     1321 acatgcggct ccaggcgatg gatcgcgccg gggtggatat cggggtcatc tcgctggccg
     1381 cgcccagtgt ctactggggc agcgacgcag acagcttgca ggccgcgcgc cagatgaacg
     1441 actacatcgc gcggatctgc cgcgagcacc cggatcgctt cgccggcatc gcgaccctcc
     1501 cctggcgctc ggcggccgac tcgctgcccg agctcgaccg ggccatcgat caactggggc
     1561 ataagggcgt gatggtgctg gccaacatcg agggccggcc gctgaccgat cccacctacg
     1621 cgccgatctg ggaggcgatc gaccggcgcg ggctggcggt gttcgtgcat cccatcaccc
     1681 cgccgggggc ggatcagatg gacatgctcg actacaacct ggtcgcctcg gtcggcttca
     1741 tgatcgacac gacgctcgct ttcgcccgca tgatctacga cggcttcttc gaccgctacc
     1801 ccaacatcag catgatcgcg ccgcacgcgg gggccacgct gccctacctc gccggccggc
     1861 tcgaccagtg ctgggacaag atgcctgcct gccgggtcaa catcagcgag ccgccctcca
     1921 gctacctgcg gcgcatctac tacgacaccg tggcctacgc gcagaacgcc atcgatctct
     1981 gcctggcggt gggcggtccc agccagcttc tctacggctc cgactacccg cacaacataa
     2041 gcgacatggc cggctgcctg gcgcgcgccg aagcgctgcc gggcgacgtg gtggaagacg
     2101 tcaagagccg caacgccatg cgcatcttcg ggttgtaggc ggagggtggc cctcatcccc
     2161 cccgcccttt ctcccaattc tgggagaagg ggagcatgta acggtggcgt gctcccctcg
     2221 cccagtattg ggagagggcg gggtgagggc cggcctaccc gggcgcgccg tccgcgccgt
     2281 gctcctggtc cttcgaggcg atgccgtgga gcacgatcga ggtgatcagt cgcgcggcgg
     2341 cggcggggtc ggacgggatc tggaccagcg agtcgctcag cgacggcatg accgccgcca
     2401 ccaacgtgtg gaagaagagg aaggcgccga gccggggtga gatatcgcga atctcgccgt
     2461 gggcggcggc ctgctcgaag agcccggcga gcgtgcccac gaaggcatcc agctcctga
//
LOCUS       DASUBI010000685         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_190337, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000685 DASUBI010000000
VERSION     DASUBI010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190337"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFU72_13275"
     CDS             complement(<1..438)
                     /locus_tag="VFU72_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5424510.1"
                     /translation="MTQSDDLSLMFLAQGMQSARSIAETLAGFIAEAQQTLDIAIYDV
                     HLTAETGQIILGALRQRAAAGVQIRIAFDEASSAAVSPPLGMDPPEPGTLEFIQALGL
                     PARSIGGPKLMHQKYLVRDAQLPEATVWTGSLNLTDDAFSLQEN"
     gene            631..909
                     /locus_tag="VFU72_13280"
     CDS             631..909
                     /locus_tag="VFU72_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424511.1"
                     /translation="MSTVALLLERLRADGRFVQDHDSYRLEVAAGRPDSICFTLQEFG
                     PQGAQITLGEGFSLDERLVFEQRRLPEPEIERIIRGVLMSQPELVGAN"
     gene            1003..3072
                     /locus_tag="VFU72_13285"
     CDS             1003..3072
                     /locus_tag="VFU72_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5424512.1"
                     /translation="MPNRLINESSPYLLQHAHNPVDWYAWGDEALDAARRQDRPIFLS
                     IGYSACHWCHVMAHESFENPEIARYLNEHFINIKVDREERPDLDSIYLMAVQLLTGHG
                     GWPLSVFLTPAGEPFYGGTYFPPEDRRAGPSAVMPGFSRVLTAVVEAYRDRRTEVSQA
                     VNDIQAHLTQLYAAVPETARLGSGVLDEATRQLEMQFDGINGGFGGAPKFPPSMVLEF
                     LLRAWLRTGRQNLRDMATFTLASMAHGGIFDQAGGGFHRYTVDGVWLVPHFEKMLYDN
                     ALLSRDYALAWQITGEELFRRVAGATYDYVLRELTAPSGGFYSAQDADSGGEEGAFYV
                     WTPGEITAAVGPEDAPLVMRAFDVTPEGNFEGKNVLTMALAPEALATEFGITTERVGA
                     IIAGARIKLRQWREAERVWPGRDEKVLTAWNGLMLRSLAEGGVILGRLDLIAAAERNA
                     RFIREALMVEGRLLHVWKDGVARVGGFLEDYANLLSGLIALYEATFDAEWIRWARRLA
                     VRMLAEFWDEERGGFFDTAASDEPLIVRPKEVFDSAVPSGNSEAAEALLRLALLLNDD
                     DLRQRAIVILEQHGQAAARQPTGFGRLLTAYDFALAPAREIALAGDPTDPRTQALLDV
                     LRRRYLPWQVLALRHPGNETEPAIIPLLEAREPIDGQPAAYVCQNYSCRLPVTTPEAL
                     AAELGLE"
BASE COUNT          498 a         1035 c         1085 g          529 t
ORIGIN      
        1 attttcttgc agcgagaagg cgtcgtcggt caggttcagc gagccagtcc agaccgtggc
       61 ttcgggcagt tgggcatcgc gtactagata cttctggtgc atcagcttcg gcccgccgat
      121 cgagcgcgcc ggcaggccca gcgcctggat gaactcgagc gtgcccggct ccggggggtc
      181 catccccagg ggtggactga ccgcggccga gctggcctca tcgaaggcga tgcggatctg
      241 gactccggcg gctgctcgct ggcgcagcgc gcccaggatg atctgccccg tctccgccgt
      301 caggtgaacg tcgtagatgg cgatgtcgag tgtctgctga gcctccgcga tgaagccggc
      361 cagcgtctcg gcgatcgagc gcgcgctctg catcccctgc gccagaaaca tgagcgagag
      421 atcgtcgctt tgcgtcacgt gtcctcctcg tccgtccgtc acacgatgca cggttggcta
      481 cgcgctagga ccgggatcgg ctgcccttgg ttagccggcg cgtcacggca cgaaacctgc
      541 tcaatgaacc ccagggttac aacctgtcga tcgcgagatc gccgccacaa cggcgtggcg
      601 gctcgcgatc accaccagta aggatgaggg atgagcaccg ttgcgctatt gttggaacgt
      661 cttcgcgccg acgggcggtt cgtgcaggac catgattcgt accgcctcga agttgccgcg
      721 ggacgtccag attccatctg tttcaccctg caagagttcg gtccgcaggg cgcccagatt
      781 accctgggcg aagggttcag tctcgacgag cgcctcgtct ttgagcagcg gcggctcccc
      841 gaaccggaga tcgaacgtat tatccgcggc gtcctcatgt cgcagccgga gctggtcggc
      901 gcgaactgac cagcaggata ccggtgcgag ccgcggcggg gccaatgagg tgccgccgcg
      961 gctcttttcg tttggaaggt tcgcaaaagt tcggagagat cgatgcccaa ccgtctgatc
     1021 aacgagagca gcccttacct cctccagcac gcgcacaacc cggtcgactg gtacgcctgg
     1081 ggcgacgagg cgctggacgc cgcccggcgg caggatcggc cgatcttcct cagcatcggt
     1141 tattctgcct gccactggtg ccatgtgatg gcgcacgagt cgttcgagaa tccggagatc
     1201 gcccgctatc tgaacgaaca ctttatcaac atcaaggtcg atcgggaaga gcgccccgac
     1261 ctcgacagta tctacctgat ggcggtgcag ctcctaaccg ggcacggcgg ctggccgctc
     1321 tcggtcttcc tgacaccggc cggcgagccc ttctacggcg gcacctactt cccgccggaa
     1381 gaccggcgcg cggggccgag cgcggtcatg cccggtttct cccgcgtgct gaccgcggtc
     1441 gtcgaagcct accgcgatcg ccgtaccgag gtgtcccagg cggtgaacga catccaggcg
     1501 cacctgacgc agctctacgc ggccgtgccg gagacggcgc ggctgggctc gggtgtactg
     1561 gacgaagcca cccgacagct cgagatgcag ttcgacggga tcaacggcgg cttcggcggg
     1621 gcgcccaagt tcccgccctc gatggtgctg gagttcctct tgcgcgcctg gctgcgcacc
     1681 gggcggcaga acctgcgcga catggcgacc ttcaccctgg ccagcatggc acacggcggc
     1741 atcttcgatc aggccggcgg cggctttcat cgttacacgg tcgacggagt ctggctggtg
     1801 ccgcatttcg agaagatgct ctatgacaac gcgctcctct cgcgagacta cgcgctggcc
     1861 tggcagatca ccggtgagga gctctttcgc cgcgtggccg gcgccaccta cgattacgtg
     1921 ctgcgcgagc tgaccgcgcc gagcggcggc ttctactcgg cccaggacgc tgacagcggc
     1981 ggtgaggagg gcgccttcta cgtctggact ccgggcgaga tcacagccgc cgtcggaccg
     2041 gaggatgcgc ccctggtgat gcgcgccttc gacgtaacgc cggaagggaa cttcgagggc
     2101 aagaatgtgc tgaccatggc gctggcgccg gaggcgctag ccacggagtt cggcatcacg
     2161 acggagcggg tgggcgccat tatcgccgga gccaggatca agctgcgcca gtggcgtgag
     2221 gccgagcgcg tttggccggg gcgagacgaa aaggtgctga ccgcctggaa cggcctgatg
     2281 ctgcgctcgc tggccgaggg cggtgtgatc ctgggacggc tggacctgat cgcggccgcg
     2341 gagcgaaacg cgcgcttcat ccgcgaggcg ctcatggtcg agggccggct gctccacgtc
     2401 tggaaggacg gcgtcgcgcg cgtcggcggc ttcctggagg attacgccaa tctcctcagc
     2461 ggcctgatcg cgctctacga ggcgaccttc gatgcggagt ggatccgctg ggcgcgacgc
     2521 ctggcggtgc gcatgctggc cgagttctgg gacgaggagc gcggcggctt cttcgacacc
     2581 gccgcgagcg atgagccgct gatcgtgcgc cccaaggagg tcttcgatag cgccgttccg
     2641 agcggcaact cggaggccgc cgaggcgctg ttgcggctgg cgctgctgct caatgacgac
     2701 gatctgcgcc agcgcgcgat cgtgatcctg gagcagcacg gccaggctgc cgcgcgccag
     2761 ccgaccggct ttggccggct gctgacggcg tacgacttcg cgctggcgcc ggcacgcgag
     2821 atcgcgctgg cgggcgaccc gactgatccg cgaacccagg cgctgctcga tgtgctgcgg
     2881 cggcgctacc tcccctggca ggtgctggcg ctgcgccacc ccggcaacga gaccgagccc
     2941 gccatcattc cgctgctgga ggcgcgagag ccgatcgacg gccaacccgc cgcctacgtt
     3001 tgccagaact acagctgtcg cctcccggtc acgacgcccg aggcgctggc ggccgagctg
     3061 gggctggaat agacggatga ggccgcctct gtgtctcccc tctcccagca ttgggagagg
     3121 ggtcgggggg cgagggcgcg tctacgg
//
LOCUS       DASUBI010000686         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_9907, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000686 DASUBI010000000
VERSION     DASUBI010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9907"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFU72_13290"
     CDS             complement(<1..440)
                     /locus_tag="VFU72_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5424513.1"
                     /translation="MAPPLAHAGLQAELEKTLGDFHLKVALGARPGEPVALLGPSGSG
                     KSTVLRLLAGLLRPDAGRIQVGERAWFDADHGIDVAPERRDVGMVFQQYVLFPHLTAL
                     ENVAFGLRRLGMKREAARELARRELERLGVGELAGQRPAQLSGG"
     gene            complement(425..1231)
                     /locus_tag="VFU72_13295"
     CDS             complement(425..1231)
                     /locus_tag="VFU72_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424514.1"
                     /translation="MWHRGVWGGAATGLAWVLAVLCVLLFALPLLALVVHQSPGTVLS
                     ALLRPRVIEALQLSALTTTIATVAAVVLGIPVAYLLARRKLPFSSAIATLLTLPTVLP
                     PVVAGVALLVAFGRRGLLGQYLLLAGISIPFTTVAVILAQLFVAGPFFISAARAAFEN
                     LDPAYEEAAMTLRATALARTLLVAIPLTAPQLVVGASLTWARALGEFGATITFAGNLP
                     GVTQTLPITTYLTAQNDLDTAVAISVVLLVVSGLLLAAMPRLGGSLWRPR"
     gene            complement(1478..2320)
                     /gene="modA"
                     /locus_tag="VFU72_13300"
     CDS             complement(1478..2320)
                     /gene="modA"
                     /locus_tag="VFU72_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5424515.1"
                     /translation="MSAKGWSRGILPSGRLALLACLLLVAGCGRGAQSSSGSSPSAGG
                     GTLTVFAAASLTEAFGDLKPVFEHAHPGVTLRYNFAGSQELVAQIEQGAPADVFASAD
                     QGHMDALVKESLVEPAQTFAHNRLVVITPASGSPVHTLHDLAASGVKLDVADASVPVG
                     GYTLQMLDKLANDPAYGPNFKQQALDRVVSHEDNVKAVVTKVSLGEVDAGVCYATDVT
                     ATLRDKVRTIDVPDAYNVIASYPVAPVKASKQADLARAFIQMLVSSEGQKVLAAHGFS
                     PARP"
     gene            complement(2310..3152)
                     /locus_tag="VFU72_13305"
     CDS             complement(2310..3152)
                     /locus_tag="VFU72_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424516.1"
                     /translation="MTIDEQRGPGDGRHHVRSMLMRESLLDKQVMSSTESPVTRMLPF
                     CHVIKIGGRSIMDGGREVVYPLIDAIGGALEDHKLIIGTGGGIRSRHVFSIGIDLGMP
                     TGVLAQLAIADALGNAHMLGALLAPYGVVAIPPEIFGHLLPLFVNAVPGVIFNGDPPF
                     SLWEHPPAIGRIPPHRSDAGSYLLAECFGCATLTLIKDVDGLYDKDPKEHPDAEFIPE
                     ITVSELRARNLPTLPFDRVLIDLLATSRLVTQFQVINGTRPELLAAALRGEHVGTIVR
                     ADER"
     gene            complement(3178..>3774)
                     /locus_tag="VFU72_13310"
     CDS             complement(3178..>3774)
                     /locus_tag="VFU72_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424517.1"
                     /translation="MIIGTGAGTRARHAYSVGLDLGMPTGVLSVLGTFVSMQNARMIH
                     YLLAKHGVPFIEPAQFAQLPLYLAERQAAVFFGMPPYTFWQPNPEVGRIPPHRTDTGA
                     YLVSEVFGARSMIYVKDEDGLYTADPKKDPSARFIPEITVAELEALDLADVVVERSVL
                     TLLKQAVHRRSIQVINGLKPGTLSRALAGEHVGTIIRA"
BASE COUNT          631 a         1309 c         1270 g          564 t
ORIGIN      
        1 tgcccgccgg agagttgcgc gggacgctgc cccgccagct cgcccacgcc gagccgctcc
       61 agctcacgcc gcgccagctc ccgggcggcc tcgcgcttca tccccagccg ccgcaggccg
      121 aaagcgacgt tctccagcgc cgtcagatgt ggaaagagga cgtattgctg gaagaccatc
      181 cccacgtcgc gccgctcggg cgccacgtcg atcccgtgat cggcgtcgaa ccaggcccgc
      241 tcacccactt gaatccgacc cgcgtcaggc cgcagcagcc ccgccagcag acgcagcacc
      301 gtgctcttgc ccgagccgct ggggccaagc agcgccaccg gctcgcccgg ccgcgcgccg
      361 agcgcgacct tcaggtggaa gtcgcccagc gtcttttcca attccgcttg cagaccggcg
      421 tgcgctaacg ggggcgccac aggctgcctc ccagccgcgg catcgccgcg agcagcagac
      481 ccgagacgac cagcagcacc accgagatcg cgaccgcggt gtcgagatcg ttctgcgccg
      541 tcaggtatgt cgtgatcggc agcgtctgcg tcactcccgg taagttcccg gcgaaggtga
      601 tcgtcgcgcc gaactcgccc agcgcgcgcg cccaggtcag gctcgcgccg accaccaact
      661 gcggcgcggt cagtggaatg gcgaccagca gggtgcgcgc cagcgccgtc gcgcgcagcg
      721 tcatggccgc ctcttcgtag gcgggatcga ggttttcgaa ggccgcgcgg gcggcgctga
      781 tgaagaacgg ccccgccacg aagagctgcg ccagaatgac ggccacggtg gtgaagggga
      841 tcgagatccc ggccagcagc aggtactggc ccagtagtcc gcgccgcccg aaagctacca
      901 gcagcgccac gccggccacc accggcggca gcacggtcgg cagcgtcagc agcgtggcga
      961 tcgcgctgct gaagggcagc ttgcgccgcg ccagcaagta ggccacggga atgccaagca
     1021 cgaccgcggc gacggtggcg atggtggtcg tcagcgccga gagctgcaac gcctcgatga
     1081 cgcgcggcct gagcagcgcc gagagcacgg tgcctggcga ttggtgcacc accagggcga
     1141 gcagcggcag ggcgaagagg agcacgcaga ggaccgccag cacccaggcc aggccggtcg
     1201 cggcgccgcc ccagacccct cggtgccaca ctcgcgagct caatcgtgac atcatctcgg
     1261 ttcgcgttat cggcggtgcc atgacggtct ctcgtccagc ctgcttcggt gacccacgtt
     1321 acaacgacag gcggcgcgtc gcgggggtgt gggaatttgc gaggaatagg tgcgaaaccc
     1381 ttcgccacct ctgtcacttg gtagccgcag ccggaacatt tctcccgcgg tcccgcggcg
     1441 caccggcctt gccccaaccg cctcagtggg ggcaaatcta tggcctagcc ggggagaatc
     1501 cgtgcgctgc gagcaccttt tgcccctccg agctgaccag catctggatg aaggcccggg
     1561 ccagatccgc ctgcttcgat gccttcaccg gcgccaccgg atagctggcg atgacattgt
     1621 aggcgtccgg cacgtcgatc gtccgcacct tgtctcgcag cgtcgccgtg acatcggtgg
     1681 cgtagcagac cccggcatcg acctcgccca ggctcacctt ggtcaccacc gccttgacgt
     1741 tgtcctcgtg gctgaccacc cgatccagcg cctgttgctt gaagtttggc ccataggcag
     1801 ggtcgttggc cagcttgtcg agcatctgca gtgtgtagcc gccaacgggc accgaggcat
     1861 ccgcgacgtc gagcttgacg ccactggcgg caaggtcgtg caatgtatgc accggcgacc
     1921 ccgatgccgg ggtgatcacc accagccggt tgtgggcgaa cgtctgcgca ggctcgacca
     1981 gactttcttt gaccagcgcg tccatgtggc cctggtcggc cgaggcgaag acatcggccg
     2041 gcgcaccttg ctcgatctgc gccaccagct cctgcgaacc ggcaaagttg tagcgcagcg
     2101 tgacaccggg gtgcgcgtgc tcgaagaccg gcttgagatc gccgaatgcc tcggtcagcg
     2161 aggcggcggc gaagacggtg agcgttccgc cgccagcgga aggcgaactg ccgctcgagc
     2221 tctgcgcgcc ccgcccgcag ccggctacca gcagcaggca ggccagcagc gcgagccggc
     2281 cggacggtag gatacccctc gaccagccct tagcgctcat ccgcgcgcac gatggtgccg
     2341 acatgctcgc cgcgcagcgc cgccgccagc agttcgggcc gggtgccgtt gatcacctga
     2401 aattgcgtga ccaggcgcga tgttgccagc aggtcgatca gcacgcggtc gaagggcaga
     2461 gtcggcaggt tgcgcgcgcg caactccgag acggtgatct ccgggatgaa ctccgcgtcc
     2521 gggtgctcct tcggatcctt gtcgtagagc ccatcgacat ccttgatcag cgtcagcgtg
     2581 gcgcagccga agcactcggc cagcaggtag ctgccggcgt cgctgcggtg gggcgggatg
     2641 cggccgattg ccggcgggtg ttcccaaagc gagaagggcg ggtcgccgtt gaagatcacg
     2701 cccggcaccg cgttgacgaa gagcggcagc agatgcccga agatctccgg cggaatcgcc
     2761 accacaccgt agggcgccag cagcgcgccc agcatatgcg cgtttcccag cgcgtcggcg
     2821 atggcgagct gcgccagcac gccggtcggc atgcccaggt cgatcccgat cgagaagacg
     2881 tggcgcgagc gaatgccgcc gccggtgccg atgatcagct tgtgatcctc tagcgcgccg
     2941 ccgatggcgt cgatcagcgg gtagaccacc tcgcgcccgc catccatgat cgagcggccg
     3001 ccgatcttga tcacatggca gaagggcagc atgcgcgtga ccggagactc ggtcgaggac
     3061 atcacctgct tgtcgagcag gctctcgcgc atcagcatcg agcgcacgtg gtgtcgcccg
     3121 tcccccggcc cgcgctgttc gtcaatagtc acggtcaatc cctctcgcgc tcaacgctca
     3181 ggcacgaatg atcgtgccga catgctcgcc ggccagggca cggctcaagg tacccggctt
     3241 cagcccgttg attacctgga tcgagcggcg gtggaccgcc tgcttgagca gcgtcagcac
     3301 gctgcgctcg accaccacgt ccgccagatc cagcgcctcc agctccgcga ccgtgatctc
     3361 ggggatgaag cgcgccgagg gatccttctt ggggtcggcg gtgtagagcc cgtcctcgtc
     3421 cttgacgtag atcatcgagc gggcgccgaa gacctcactc accaggtagg cgccggtatc
     3481 ggtgcggtgc ggtgggatgc gcccgacctc ggggttcggc tgccagaagg tgtagggcgg
     3541 catgccgaag aagacggcgg cctggcgctc ggccaggtag agcggaagct gggcgaactg
     3601 cgccggctcg atgaagggca cgccgtgctt ggccagcagg tagtggatca tgcgtgcgtt
     3661 ctgcatgctg acgaaggtgc cgagcacgct cagcacgccg gtcggcatgc ccaggtcgag
     3721 gcccacgctg taggcgtgcc gagcccgcgt gcccgccccg gtgccgataa tcat
//
LOCUS       DASUBI010000687         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_698804, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000687 DASUBI010000000
VERSION     DASUBI010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698804"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFU72_13315"
     CDS             complement(<1..275)
                     /locus_tag="VFU72_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5424518.1"
                     /translation="MERSTGRAESNPELVAAIRDEVVRAGRITFARFMELALYHPEHG
                     YYRAAAVRPGRAGDFLTAPEAHPIFGHALARQLDEMWHVLGQPAPFIL"
     gene            317..970
                     /locus_tag="VFU72_13320"
     CDS             317..970
                     /locus_tag="VFU72_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5424519.1"
                     /translation="MNQEPEAEQGTIDLGGRLWISQRRLGRMAGVLRRRQPDLTVVIE
                     NVHDPHNISAVLRSCDAVGVDTVHLVYTQEERPQLSRISSASALKWLQVVDHPSIAAC
                     YAALRRQGCRIYATHLGEASLDLYDLDLAQPSALVFGNEQRGVSPEAVAAADATMVIP
                     MMGMVQSLNISVACAVSLYEALRQRRLAGAYAAPKLGQGELDARLTRWLEREGRITP"
     gene            complement(960..1445)
                     /locus_tag="VFU72_13325"
     CDS             complement(960..1445)
                     /locus_tag="VFU72_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5424520.1"
                     /translation="MSETDALLRVGLALLFGAAIGIERELNEKPAGLRTYMLVSEGAA
                     LFMISSILLGQEFNTGQSAPIDISRIASTIVTGVGFLGAGMILQSRQRVYGLTTAAGI
                     WVAAAVGMLVGAGYLFISLAGTVLAVLTLILPRLIEQRLGTWYAGRYHGEPPDPDEPR
                     A"
     gene            1588..2076
                     /locus_tag="VFU72_13330"
     CDS             1588..2076
                     /locus_tag="VFU72_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5424521.1"
                     /translation="MRETTSRRLPSAPARRPPRTRDLRQRAAGLHARHTARLLRFALV
                     GGAGVLVNYAILAGLVELGHLPRLLAAALATEGAILSNFTLNNSWTFGDVACHHSLPA
                     RAARYNFFALGGLLISVAVLGVLVYVFGLYYLIANLFAIGAATLWNYAANARWTWRLN
                     RD"
     gene            2142..2735
                     /locus_tag="VFU72_13335"
     CDS             2142..2735
                     /locus_tag="VFU72_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5424522.1"
                     /translation="MVRLLLLVSLAVALVACSESPKLAGTAFDPPQPAPDFTLTDQRG
                     QSVQLSSYRGDAVALTFIYTNCTDVCPLIAEKLRRAYDALPPATQQKVQLLAVTVDPA
                     RDDRQALQDFSAKHQLADNPHWHAMTGDQSVLEQVWKGYYIYPGQMMGTDAATPGSTT
                     VHTDAIVFIDPKGERRALLDSAADPNAIAANLKALAG"
     gene            2806..>3146
                     /locus_tag="VFU72_13340"
     CDS             2806..>3146
                     /locus_tag="VFU72_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424523.1"
                     /translation="MTVIDWLLASDPSIRWQVLRDLTDAPAAAVAAERARVASEGAGV
                     RLLALQAADGSWGGAAWNPGGDSTMHALTLLRELGLDPASEEARRALGLVRDRVTWQG
                     CGPPECNDNPF"
BASE COUNT          468 a         1117 c         1111 g          450 t
ORIGIN      
        1 aggatgaagg gcgccggctg gccgagcacg tgccacatct catcgagctg gcgggccagc
       61 gcgtggccga agatcgggtg ggcctccggc gcggtcagga agtcgcccgc gcgcccgggc
      121 cggacggcgg cggcgcggta gtagccgtgc tcggggtggt agagggccag ctccatgaag
      181 cgcgcgaagg tgatccgacc cgcgcgcacg acctcgtcgc ggatcgccgc gaccagctcg
      241 gggttgctct cggcccggcc cgttgagcgc tccatgccat aattgtacgg tgtgcgggcg
      301 agccagaatg gatgccatga accaggagcc ggaagcggag caggggacga tcgacctcgg
      361 cgggcgcctc tggatcagcc agcggcggct ggggcgcatg gccggcgtgc tgcgtcgtcg
      421 ccagcccgac ctcaccgtgg tcatcgagaa cgtgcacgac ccgcacaaca tcagcgccgt
      481 gctgcgcagt tgcgacgcgg tcggcgtcga caccgttcac ctggtctaca cccaggagga
      541 gcggccgcag ctcagccgca tcagctcggc cagcgcgctc aagtggctgc aggtggtcga
      601 tcaccccagc atcgccgcct gctacgcggc gctgcgccgg cagggctgcc gcatctacgc
      661 cacccacctc ggcgaggcca gcctcgatct ctacgacctg gatctggcac agcccagcgc
      721 gctcgtcttc ggcaacgagc agcgcggcgt ctcaccggag gcagtggcgg cggccgacgc
      781 gacgatggtc attccgatga tggggatggt gcagagcctg aacatctcgg tggcctgcgc
      841 cgtctcgctc tacgaggcgc tgcgccagcg gcggctggcc ggcgcctacg ccgcgcccaa
      901 gctcggccag ggcgagctcg acgcccgcct gacgcgctgg ctggagcggg agggccgcat
      961 cacgccctag gctcgtccgg gtcgggcggc tcgccgtgat agcgtccggc gtaccaggtg
     1021 cccagccgct gctcgatcag gcgcggcagg atcagggtga ggaccgccag caccgtgccg
     1081 gccagcgaga tgaagaggta gccggcgccg accagcatac cgaccgcggc cgccacccag
     1141 atgccggcgg cggtggtgag cccgtagacg cgttgccgcg actgcaggat catgccggct
     1201 ccgaggaagc cgaccccggt gacgatggtg gaggcgatgc ggctgatatc gatgggcgcc
     1261 gactggccag tgttgaactc ttgccccagc aggatcgagc tgatcatgaa gagcgccgcg
     1321 ccttcggaca ccagcatgta ggtgcggagc cccgccggtt tctcgttcag ctcgcgctcg
     1381 atcccgatgg cggcgccgaa gagcagcgcc agcccgacgc gcagcagggc atccgtctcg
     1441 ctcaccacca cagcctcctc tcctggtacc atgcctcgtg tgaggctgcc cgtgattctg
     1501 cgcctggcat caagcctgcg caacgcgggc ggactggagg agatgagcgg gcgccgcgcg
     1561 ccctcgacga caggtgatga agagagcgtg agagagacaa cgagccgccg gctgccgagc
     1621 gcgccggcgc gacgaccgcc gcgcacgcgt gatctgcggc agcgtgcggc agggctgcac
     1681 gcgcggcaca ccgcccggct gctgcgcttc gcgctggtcg gcggcgcggg ggtgctggtg
     1741 aactacgcga tcctggctgg gctggtggag ctgggccatc tgccgcgcct gctggccgcc
     1801 gcgctggcga ccgagggcgc catcctcagc aacttcaccc tgaataacag ttggaccttc
     1861 ggcgacgtgg cctgccacca ctcgctgccg gcgcgggcgg cgcgctacaa cttcttcgcg
     1921 ctgggcgggc tgctgatctc ggtcgccgtg ctgggcgtgc tggtctacgt cttcgggctg
     1981 tattacctga tcgccaacct cttcgccatc ggcgccgcca ccctctggaa ctacgccgcc
     2041 aacgcccgct ggacctggcg cctgaatcgg gattgaggcg ccgggtggta gcatacaggg
     2101 cggccgcggt tcgtctgggc gtgcgctggg aaggattcgc tgtggttcgc ctgctgctcc
     2161 tcgtctcgct ggccgtggcg ctagtcgcct gtagcgagag ccccaagctg gccggcaccg
     2221 ccttcgatcc cccgcagccg gcgcccgact tcaccctcac cgaccagcgc ggccagagcg
     2281 tgcagctcag cagctaccgc ggcgacgccg tggcgctcac cttcatctac accaactgca
     2341 ccgatgtctg cccgctgatc gccgagaagc tgcgccgcgc gtacgacgcc ctgccgcccg
     2401 cgacgcagca gaaggtgcag ctgctggctg tcaccgtcga cccggcgcgc gacgaccggc
     2461 aggcgctgca ggacttctcg gccaagcacc agcttgccga taacccccac tggcacgcca
     2521 tgaccggcga ccagtccgtg ctggagcagg tctggaaggg ctactacatc tatcccggtc
     2581 agatgatggg gaccgacgcc gccacgccgg gttcgaccac ggtccatacc gacgccatcg
     2641 tcttcatcga tcccaaaggg gagcgccgcg cgctgctcga cagcgcggcc gacccgaacg
     2701 cgatcgccgc caacctcaag gcgctggccg gctagtgcac acgcgccggc tccagcattc
     2761 cgaggtaagc gctggatcgc cgcagcctgc cgtagaggga gatccgtgac cgtcatcgac
     2821 tggctgctcg cgtcggaccc gtcgatccgc tggcaggtgc tgcgggatct gaccgatgcg
     2881 cccgcggcgg cggtcgcggc cgagcgcgcg cgggtcgcca gcgagggcgc cggtgtgcgg
     2941 ctgctcgccc tgcaggcggc cgacggatcc tggggcggcg cggcctggaa ccctggcgga
     3001 gactccacca tgcacgccct gacgctcctg cgcgagctgg gcctcgaccc cgccagcgag
     3061 gaagcgcggc gcgcgctggg cctcgtgcgc gaccgggtga cctggcaggg gtgcggcccg
     3121 ccggagtgca acgacaatcc cttctt
//
LOCUS       DASUBI010000688         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1292182, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000688 DASUBI010000000
VERSION     DASUBI010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292182"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFU72_13345"
     CDS             <1..883
                     /locus_tag="VFU72_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional lytic transglycosylase/C40 family
                     peptidase"
                     /protein_id="HEU5424524.1"
                     /translation="TAALSALNPARPAGIGNGTSQAIPAEYRAMMERASAASCGVSWN
                     VLAAIGKVESDFTPGSVGPDLPQFAGTPDEHALGLMQFLPSTYRDLAPRVDAATGKAL
                     GEAGIWDAESAIFAAAFYLCDNGAPGDLRAALYAYNHADWYVDQVLAQAAAYGLGDTG
                     NDAGAGPNGDVVTLARQYLGWPYVWGGADPTTGFDCSGLVQWLYGQRGVALPRTAQAQ
                     FDGTARLAPEELQPGDLVFFAQTYADAANWITHVGLYIGDGQMINAPTEGEVVQTMPV
                     FTGFWGDHYAGGGRVRR"
     gene            1031..1270
                     /locus_tag="VFU72_13350"
     CDS             1031..1270
                     /locus_tag="VFU72_13350"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424525.1"
                     /translation="MEAIQGLAQNIVTGLRLLGGVVVVGCLIWSGILWMTAAGNPRHI
                     ETARAAFVGSFVGFAIVAAAPQIASFISGALGGWQ"
     gene            1319..1759
                     /locus_tag="VFU72_13355"
     CDS             1319..1759
                     /locus_tag="VFU72_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424526.1"
                     /translation="MIPARHIIPTGLAVEDRFITYGAFALSLRQCFLLLGGAATGYGG
                     VWQGLSSLPVPLRLGAALTPSIIALTVAFVRPGGRQPEDWLFILARYWCLPRRTVWRP
                     RAPEPVDWASANTGWAGTTIRPLWLVEEPGRAAEAGAPWPPPCC"
     gene            1738..2508
                     /locus_tag="VFU72_13360"
     CDS             1738..2508
                     /locus_tag="VFU72_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HEU5424527.1"
                     /translation="MATTLLLIVILALVAIVCRALSGGAAGQPRGVQQEHLALAAIAD
                     SIVTITDGRRRAVLEVGSVNFAAHSPAQQRELVAGYGAALASLTYPVQVLVRAAPLDL
                     APYLDEREARALQEPHEQLRRLNRDRIAFVRRLASKQTFLDRRFYLIVPADDSHPVTR
                     WRVPRPGRQRTPERRSADGDTVASQQLAARCADLASQLGRCGLTAQRLDDAELAELAY
                     ACWCPDLAQIQRLRHDLRDEHFIVTRAPTDIPAERMVP"
BASE COUNT          381 a          915 c          892 g          438 t
ORIGIN      
        1 gaccgcagcc ctcagcgcgc tgaacccggc acgccccgcc ggaatcggca atggcacgtc
       61 ccaggccatt ccggccgagt accgggcgat gatggaacgc gcgtcggccg cgagctgtgg
      121 cgtgtcgtgg aatgtgctgg cggcgatcgg caaggtcgaa tcggacttca cgcctggctc
      181 cgtcggcccc gacctaccgc agtttgccgg cactccggac gagcatgcgc tcggcctgat
      241 gcagtttctc cccagcacct accgcgacct ggcgccgcgc gtggacgcgg cgaccggcaa
      301 ggcgctgggc gaggcgggga tctgggacgc cgagtcggca atcttcgccg ccgcctttta
      361 tctctgcgac aacggcgcac cgggtgacct gcgcgccgcg ctctacgcct ataaccacgc
      421 ggactggtac gtagatcagg tgctggcgca ggcagcggcc tatggcctcg gcgataccgg
      481 caacgatgcg ggagcggggc cgaacggcga tgtcgtgacg ctggcgcggc agtacctcgg
      541 ctggccctac gtctggggcg gcgccgaccc gaccaccggg ttcgactgct ccggcctggt
      601 gcagtggctc tatgggcagc gcggtgtggc gctgccgcgc accgcccagg cgcaattcga
      661 cgggaccgcg cgcctcgcgc cggaggagct tcagccgggc gacctcgtct tcttcgccca
      721 gacttatgcg gacgccgcga actggatcac gcatgttggc ctctacatcg gcgacggtca
      781 gatgatcaac gcgcccaccg agggtgaggt ggtccagacc atgccggtct tcaccggctt
      841 ctggggcgac cattacgctg gcggtggccg ggtgcggcgg tgagggcaga tatgcctggc
      901 gaaacgtgca tcctattcca tcccgccggt tccggcaacc actgccagcg tggtccgcca
      961 tgccggacgg ccattgctgg tctggcggtg cgggtgcggc aagcgctcgc tgcggtgaca
     1021 ggagaaacct atggaagcga tccagggcct tgctcagaac atcgtcaccg gactgcgact
     1081 gctcggtggt gtcgttgtcg ttggctgcct gatctggtcc ggcattctct ggatgaccgc
     1141 cgccggcaac ccccgccaca tcgagacggc gcgagcagcg ttcgtcggtt cgttcgtcgg
     1201 cttcgcgatc gtcgcggccg ccccgcagat cgccagtttc atcagtggcg cgctcggtgg
     1261 ctggcagtga caacacatgc ccggcacacc cactgtggaa gacgtctgga gaaataccat
     1321 gattcctgcc cgtcacataa tcccgaccgg cctggccgtt gaagaccggt tcattaccta
     1381 cggcgcattt gccctctcgc tgcggcagtg cttcctgctg ctcggcggag cggccaccgg
     1441 ctatggcggc gtctggcagg ggctatccag cctgcccgtt ccgcttcggc ttggcgcggc
     1501 actgacgccg tcaatcatcg ccctgactgt cgccttcgtg cggccaggcg ggcgccagcc
     1561 ggaagactgg ctattcatcc tggcgcggta ctggtgcctg ccgcgccgca cggtctggcg
     1621 accacgggcg ccggaaccag tggactgggc gtcggcaaac accggctggg ccgggaccac
     1681 gatccggccg ctctggctgg tggaggaacc gggccgggcg gctgaggctg gtgcgccatg
     1741 gccaccaccc tgctgctgat cgtcatcctt gccctggttg ccatcgtctg ccgtgcgctg
     1801 agtggcggcg cggcgggtca gccacgcggg gtgcagcagg agcacctggc gctcgcggcc
     1861 atcgccgaca gcatcgtgac cattacggac ggacgccgtc gcgccgtgct ggaggtcggc
     1921 tcggtcaatt tcgccgcgca cagtccggct cagcagcgcg aactggtggc cgggtatggc
     1981 gcggccctcg ccagtcttac ctatcccgtg caggtgctcg tgcgcgcggc gccgctcgat
     2041 ttggccccgt atctggacga gcgcgaagcg cgggccttgc aggagccgca cgagcagctg
     2101 cgccgcctga accgggatcg tattgccttc gtgcgccggc tggccagcaa gcagacattc
     2161 cttgatcgcc gcttctacct gatcgtcccc gccgatgatt cgcacccggt gacgcgctgg
     2221 cgtgtgcctc gcccaggcag gcagcgaact ccggagcgcc gcagcgcgga cggcgacacg
     2281 gtggccagcc agcaactggc cgcgcgctgc gccgacctgg caagccagct cggccgttgc
     2341 ggtctcaccg cgcagcgact ggacgacgcc gaattggctg aactcgccta cgcctgctgg
     2401 tgtcccgacc tcgcccagat ccaacgcctc cgacacgacc tgcgcgacga gcatttcatc
     2461 gtcacgcggg cgccgacgga tattccagcc gaaaggatgg tgccctgatg gcgttcttcc
     2521 tcagcattcg ccgccggaag ccacccctgc ccggcgctgt ggccggtggc aagcgcgatg
     2581 ccagtctcca cccgacgacc cggcggctgg cgcttgggca gcgcac
//
LOCUS       DASUBI010000689         6185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_727575, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000689 DASUBI010000000
VERSION     DASUBI010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727575"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..1118)
                     /locus_tag="VFU72_13365"
     CDS             complement(390..1118)
                     /locus_tag="VFU72_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5424528.1"
                     /translation="MTVTTRRRTTRRRPLALVAALLAALALPLLGAAPPPSGGVDDGA
                     HLFSAAALTQAAQQIRQLQLETNRGIAVVSVPSLEGQPIEQIAAATLKQRQLEVLIYI
                     AQDEHALAVEVGPNTRQALTPPEMTAIRDLMLDQFGQGNFDAGLLAGLQRLGQDLRSS
                     TAPPGGFAGTSAQQTVFLLAGALVLAAFLTILANPRAFRTRAGRYLLPGQAVAPGREE
                     EQLREAIERIPPEGPEPPQEPPVD"
     gene            1367..1876
                     /locus_tag="VFU72_13370"
     CDS             1367..1876
                     /locus_tag="VFU72_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5424529.1"
                     /translation="MAFDRATLPLSEVDRAIIHALEHDGRRSATAIAQSLGLPLSTVR
                     RRLQRLLDEGALQVVAIPDNDRLGLPVHVLMRIEADTASGDTIGEALEAMEEVRWVAM
                     VAGQFDFLVEAFFPSNLHLHAFLVQKLARIPGILRTETLTVLRLYKNSFDWSSMLEAT
                     QADSAITRR"
     gene            1964..3877
                     /locus_tag="VFU72_13375"
     CDS             1964..3877
                     /locus_tag="VFU72_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5424530.1"
                     /translation="MSQDSSNLPENGTPRVPVLAGLSRRKFLRFAAGSTALAALSTGL
                     LACGSSSSSPTTAPAATKGASGTAAPTTAGSGGSSATSTSTGGSAATSAATSAAPSGN
                     YAKNATVTIPIATTIDITLDPHKAINSLLLFNLYDYVFGGVTKYDKDAHVVPDLAAKW
                     EASQDGLTYTFHLRDGIKFANGRAVTPDDFLYSWKRSLDPKKPSPMAHFMEHLEGYEE
                     YLGGKATDLTGFKKVDDKTIQVTLTKPYNFFLSYSCTYVFWVVDKDQVQKYGDDNNSD
                     WTNHEPYGTGAWKVTKFDPATTIEMAPNENYWGERSPSVTKVSWPILKGPTGPNQALN
                     LYKGGQADIVSNFPLSLLDAVEKDYKNDIVKVTVGGTRSVAFSFTKKPFDNVLVRLAF
                     GMAIDRDTYCNTVWRGAYTPTDHYEPPSIKDYDPPKGIAYNPDEAKKVLAAAGFANGQ
                     GLPPIILYIPSDTSAEDVNRYRALADMWNKTLGANVQINTAFTNTQIIDKRTQEKGFQ
                     LELMGWINITETPQLMSEVFRTDSPYMKDRFDWGIQVPAMTYNGVTYDPTADAKKFDD
                     LMNQADIEQDPKKRNQLYHDGEALVLKDAIYVPFGNYVYRELVNPKLKGLEWGAFFYT
                     YPVSIKKNVVMEK"
     gene            3951..4892
                     /locus_tag="VFU72_13380"
     CDS             3951..4892
                     /locus_tag="VFU72_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424531.1"
                     /translation="MIGYIIRRVLYSIFVIIGVLFTVFAVARLSPVDPVKYVLMQSGR
                     NPGDVDPVEYAEMRHSLGLDRPIMVQFVDYVGEVLHGDFGTSIVNQGRTVRDIMGSGI
                     PISLQLAFMGLLLQFVVGNLVGVLAAAKQNSLFDRTVMGVAIVVGSVPQLVWGVVFIT
                     IFAVQFRWLPIRGWDHPTNWILPTLTIGIAGIASYARFGRAAVLEQANQDFVRTARAK
                     GLRQNKVLFGHILRNALVPIITFVGPSFAFLITGNFVVETMFGVPGVAYYAINSSIHG
                     DYPVMQATVLLIAIMIMTVNLLIDVLYGVIDPRIRLH"
     gene            4905..5843
                     /locus_tag="VFU72_13385"
     CDS             4905..5843
                     /locus_tag="VFU72_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424532.1"
                     /translation="MGAATRTEAQEADRAPGLDRELKVRKNWQMALTRFRHNGPAMAG
                     LIVALFSIVVALIAPLIAPYDPAATDFVNINVWPNSKHLFGTDGSGADIFSITILSLR
                     TSYIVAVITQVITLVAGMTVGMVAGFFGGWIDGVLSRLIDVLFSFPNILVAILIAGTF
                     GVPMYQRFGPAGRLYLTVGALSLFNWAGLARVIRSEVLRLREQTYVEASLASGARGGW
                     IITRHMLPNLMGIAAVLSSLGVGFAFTLEAVLSYLGLGVTAPTPSLGRLIQAGQVYID
                     PFWYQFVFPAVVLAIMVLAYAFIGDGLRDALDPRSQ"
     gene            5949..>6185
                     /locus_tag="VFU72_13390"
     CDS             5949..>6185
                     /locus_tag="VFU72_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424533.1"
                     /translation="MSEETMDLGRIAEEWVKAGLSRRKLLQFIAGGTSASALAAVVAA
                     CGGSSAPATTAATGTSGASTQAPATSAPGAASPST"
BASE COUNT         1032 a         2116 c         2016 g         1021 t
ORIGIN      
        1 ggccgactcc aggccgaggc taccgccaca ctccgggcac tctcccatga ttgagactcc
       61 ctccgacacg aacaccagat aaacaaaaag cggcggcgag tcttccgctc accgccgcct
      121 ggcttcctca gggttggcgc cgctaggcgc ccggtccact gacagcgaga catgcgagag
      181 cggccccacg cctggcgggg gcgctggcag gggtagggac gggtttcctg gcgcgaccgg
      241 acaccgcggg ggcgccgctc ggcgcaccgc tcgcgatctc gtgatcctgc cgcatcaacg
      301 gatccatctc tcccctgtgc tctgatctca gtctgctgtc tcgcggatca gccgaccgat
      361 cgtaccacac cgtgacaaga gtgcaagggt caatcgaccg gcggctcttg cggcggctcc
      421 ggcccctcgg gcggaatgcg ctcgatcgcc tcccgcagtt gctcctcctc ccggcctggc
      481 gcgaccgctt gccccggcag gaggtagcgc ccggcgcgcg tgcggaaggc gcgcgggttg
      541 gccaggatag tgaggaaggc ggccagcacg agcgcgccgg ccagcaggaa gaccgtctgc
      601 tgggcgctgg tgccggcgaa cccgcccggc ggggcggttg agctgcgcaa atcctgcccc
      661 agccgctgga gcccggccag cagcccggcg tcgaagttcc cctggccgaa ttgatccagc
      721 atcaggtcgc ggatggcggt catctccggc ggcgtgaggg cctggcgcgt attggggccg
      781 acctcgaccg ccagcgcgtg ctcgtcttgg gcgatgtaga tcagcacctc gagctggcgc
      841 tgcttgagcg tggcggcggc gatctgctcg atcggctgac cctccaggct gggcacactg
      901 acgaccgcga tgccgcggtt ggtctcgagc tggagctgcc ggatctgctg ggccgcctgc
      961 gtcagcgcgg cggccgagaa gaggtgcgcg ccgtcgtcca cgccgccgga cggcggcgga
     1021 gcggcgccga gcaggggcag cgcgagcgcg gccagcagcg cggccacgag cgcgagcggc
     1081 cgccggcgag tcgtgcgtct gcgcgttgtt acggtcatcc ttacaccttt ggcgcggcct
     1141 gccggaacgc gacaaccagc cagcagggat tgtgccggga agtcgcaacg ttgtccaacc
     1201 ggccaaaatt gtccgtgagg cggcggccgg cggacgattc gcttatatcg tgccgacctc
     1261 ttgacagcat tcgccatggg ctctatggtg ggcctgtccg attcttgtcg cgccgcggcg
     1321 gcggcatcgt gcgagccgaa gctcagaagc ccagggggga cgagcgatgg ccttcgaccg
     1381 tgcgacattg cccttgtccg aggtagatcg cgcgatcatc cacgcgctgg agcacgatgg
     1441 ccgtcgctcg gccaccgcca tcgcgcagtc gctcggtctg ccgctctcga ccgtgcgtcg
     1501 gcggctgcag cggctgctcg acgagggggc gctgcaggtg gtggccatcc cggacaacga
     1561 ccggctcgga ttgccggtcc acgtcctgat gcggattgag gcggacaccg ccagcggcga
     1621 cacgatcggc gaggcgctcg aggcgatgga ggaggtgcgc tgggtggcga tggtcgccgg
     1681 ccagttcgac tttctggtgg aggcgttctt cccctccaac cttcatctcc acgcctttct
     1741 ggtacagaag ctggcgcgca ttcccggcat cttgcgcacc gagaccttga ccgtgctgcg
     1801 gctgtacaaa aacagcttcg actggtcgtc gatgctggag gccacgcagg cggactcggc
     1861 catcacgaga cggtgacgcg ggccaagcgg gaacgcgggc ggcgcgtatt ggtattgttc
     1921 ggcccgtgct cgaagcgagc cggcgctggg gagtaggtgg gtcatgtctc aggattcgtc
     1981 caaccttccg gagaacggca cgccccgcgt gcctgttctc gccggcctga gccggcgcaa
     2041 gttcttgcgc ttcgcggcgg gcagcaccgc gctggccgcg ctctcgaccg ggctgctggc
     2101 ctgcggcagc agcagcagtt caccgaccac cgcgccggcg gccacgaagg gcgccagcgg
     2161 caccgccgcg ccgaccacgg ccggcagtgg cggcagcagc gcgaccagca cctcgacggg
     2221 gggcagcgcg gccacctcgg ccgctacctc ggccgcgccg tccggcaact acgccaagaa
     2281 cgccaccgtg accatcccga tcgccaccac gatcgacatc acgctcgatc cgcacaaggc
     2341 gatcaactcg ctgctcctct tcaacctcta cgactacgtc ttcggcggcg tgaccaagta
     2401 cgacaaggac gcgcacgtcg ttcccgacct ggccgcaaaa tgggaggcct ctcaggatgg
     2461 gctgacctac accttccacc tgcgcgacgg catcaagttc gccaacggcc gcgcggtcac
     2521 gccggacgac ttcctttact cctggaaacg gtcgctcgac cccaagaagc cctcaccgat
     2581 ggcgcacttc atggagcacc tcgaggggta cgaggaatac ctcggcggca aggccaccga
     2641 cctgaccggc ttcaagaagg tcgacgacaa gacgatccag gtcaccttga ccaaacccta
     2701 caacttcttc ctcagctact cctgcaccta cgtcttctgg gtggtcgata aggatcaggt
     2761 gcagaagtac ggcgacgaca acaacagcga ctggaccaac cacgagccct acggcaccgg
     2821 cgcctggaag gtgaccaagt tcgaccccgc caccaccatc gagatggcgc ccaacgagaa
     2881 ctactggggc gagcgctcgc cctcggtcac caaggtgagc tggccgatcc tcaagggccc
     2941 gaccgggccg aaccaggcgc tcaacctcta caagggcggg caggccgaca tcgtctccaa
     3001 cttcccgctc tcgctgctcg acgcggtcga gaaggactac aagaacgaca tcgtcaaggt
     3061 gaccgtcggc ggtacgcgct cggtcgcgtt cagcttcacc aagaagccct tcgacaacgt
     3121 gctggtgcgc ctggccttcg gcatggcgat cgaccgcgat acctactgca acacggtctg
     3181 gcgcggcgcg tacaccccga ccgaccacta cgagccgccg tcgatcaagg actacgatcc
     3241 ccccaagggg atcgcctaca acccggatga ggccaagaag gtgctggcgg ccgccggctt
     3301 cgccaacggc cagggcctgc cgccgatcat cctctacatc ccaagcgaca cctcggccga
     3361 ggacgtcaac cgctaccgcg cgctggccga tatgtggaac aagacgctgg gtgccaacgt
     3421 ccagatcaac accgccttca ccaacacgca gatcatcgac aagcgcacgc aggagaaggg
     3481 cttccagctg gagctgatgg gctggatcaa tatcaccgag acaccccagc tcatgagcga
     3541 ggtcttccgc accgactcgc cctacatgaa ggaccgcttc gattggggca tccaggtgcc
     3601 ggctatgacc tacaacggcg tcacctacga tcccaccgcg gacgccaaga agttcgatga
     3661 tctgatgaac caggcggata tcgagcagga tcccaagaag cgcaatcagc tctaccacga
     3721 cggcgaggcg ctggtcctca aggacgccat ctacgtgccc ttcggcaatt acgtctatcg
     3781 cgagctggtc aaccccaagc tgaagggact cgagtggggc gcgttcttct atacctatcc
     3841 ggtgtcgatc aagaagaacg tggtgatgga gaagtagcgc ggcgacggcg ggccctgccc
     3901 tcggcgtggg cagggcctcg cctcgcggcg tctagcaggg aatccaggcg atgatcggat
     3961 acatcatccg ccgtgtgctc tacagcatct tcgtgatcat cggcgtgctc ttcaccgtct
     4021 tcgcggtggc gcgcctctca ccggtcgatc cggtgaagta cgtgctgatg cagagcgggc
     4081 gcaacccggg cgacgtcgac cccgtcgagt acgccgagat gcgccactcg ctcgggctcg
     4141 accggccgat catggtgcag ttcgtcgact acgtcggtga ggtgctgcac ggcgacttcg
     4201 gcaccagcat cgtcaaccag gggcgcacgg tgcgcgacat tatgggcagc ggcatcccaa
     4261 tctcgctgca gctcgccttc atggggctac tcttgcagtt cgtggtcggc aatctcgtcg
     4321 gcgtgctggc ggccgccaaa caaaactcgc tcttcgaccg gacggtgatg ggggtggcca
     4381 tcgtggtcgg ctcggtgccg cagctcgtct ggggtgtcgt cttcatcacc atcttcgcgg
     4441 tgcagttccg ctggctgccc atccgtggct gggaccaccc caccaactgg attctgccca
     4501 ccctgacgat cggcatcgcc ggcatcgcct cctacgcgcg cttcggacgg gccgccgtgc
     4561 tggagcaggc gaatcaggat ttcgtgcgca ccgcccgcgc caagggcttg cgacagaaca
     4621 aagttctctt cggccacatc ctgcgcaacg cgctggtgcc gatcatcacc ttcgtcggac
     4681 caagcttcgc cttcctgatc accggcaact tcgtggtcga gacgatgttc ggcgtgccgg
     4741 gtgtcgccta ctacgccatc aactccagta tccacggcga ctacccggtg atgcaggcga
     4801 ccgtgctgct gatcgcgatc atgatcatga cggtcaacct gctgatcgac gtgctctacg
     4861 gggtcatcga cccgcgcatc cggctgcatt agggaggacg acgcatggga gccgctacgc
     4921 ggacggaggc ccaggaggcc gatcgcgctc cgggactcga tcgcgagctg aaggtccgca
     4981 agaactggca gatggcgctc acgcgctttc gccacaatgg gccggcgatg gcggggctga
     5041 tcgtggccct cttctccatc gtggtcgcgc tgatcgcgcc gctgatcgcg ccctacgacc
     5101 cggccgcgac cgacttcgtc aacatcaacg tctggcccaa ctcgaagcat ctcttcggca
     5161 ccgacggctc gggcgccgac atcttcagca tcaccatcct ctcgctacgc accagctaca
     5221 tcgtggcggt cataacccag gtgatcacgc tggtggcggg gatgacggtc gggatggtcg
     5281 ccggcttctt cggcggctgg atcgacggcg tcctctcgcg cctgatcgac gttctcttct
     5341 ccttccccaa catcttggtg gcgatcctga tcgccggcac cttcggcgtg ccgatgtacc
     5401 agcgcttcgg cccggcgggc cgcctctact tgacggtcgg cgcgctctcg ctcttcaact
     5461 gggcggggct ggcgcgcgtc atccgctcgg aggtgctgcg cttgcgcgag cagacctacg
     5521 tcgaggccag cctcgcctcc ggggcgcgcg ggggctggat catcacgcgc cacatgctgc
     5581 ccaatctgat ggggatcgcg gcggtgctct ccagcctggg cgtcggcttc gccttcacgc
     5641 tggaggccgt gctctcctac ctcggcctgg gtgtgaccgc gccgacgccc tcgctgggcc
     5701 ggctgatcca ggcgggacag gtctacatcg accccttctg gtaccagttc gtcttcccgg
     5761 cggtggtcct ggccatcatg gtgctggcct acgccttcat cggcgacggt ctgcgggacg
     5821 ccctcgatcc gcgctcgcag tagcggcacg aacgcgaagg tcaggcgtct ggaaaggggt
     5881 tgccggcgag tactacgacc cgtgcgcgcg tgacgaaccg tttcgttgtt cagttggggg
     5941 agtgggtcat gtcggaggag acgatggacc tcggccggat cgccgaggag tgggtcaagg
     6001 ccgggctgag ccggcgcaag ctgctgcaat tcatcgccgg ggggacctcg gccagcgcgt
     6061 tggcggcggt cgtagcggcc tgcggcggca gctcggcgcc ggccacgacg gcggcgacgg
     6121 gcacgagtgg agcgagcacc caggcgccgg cgaccagcgc gccgggcgcg gcctcgccgt
     6181 cgacc
//
LOCUS       DASUBI010000690         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_378555, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000690 DASUBI010000000
VERSION     DASUBI010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378555"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFU72_13395"
     CDS             complement(<1..318)
                     /locus_tag="VFU72_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424534.1"
                     /translation="MSQAETHKRPIRVLIVDDHEMVRSGLAAFLGLAPDLELVGEAGS
                     GEEAIRLCRELRPDVVLMDMVMPGIDGPEATRAIRAAHPETQVIVLTSFLEGDLVQHA
                     LQAG"
     gene            complement(315..3077)
                     /locus_tag="VFU72_13400"
     CDS             complement(315..3077)
                     /locus_tag="VFU72_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5424535.1"
                     /translation="MDTLHGTEQPYQAIFEASSDGLVVNDLSTGVVLAANPAFCRMHG
                     YEQMVGMYPSTFIHPDSRHLLREFARAIREGREFCCQSRDVRRDGSVFDTEVLGRGIV
                     YHGAPAMLGVVRDVTEQVRQYEELERRVAERTREIEQRHAVSQALGDLLAVVNSGRTL
                     DEILDYIVEQGRRLLGSDASAIFTPDASGRRLKVRAESGLDPAYPARTIPIGMMTTGL
                     AFSLRRPFANTAVQPIPPEDRDLTRGLELEERASYARIIHLPVSLLPVDHPRAPVYGA
                     YAGGYRALLAVPLVVKEESYGALTLYYSRPHEFGDEEVALATAFANQAALAIENAQLH
                     ARQEQRRRELEALYDADEALHRSLRVEDVLRALVDVANGILASDAAAVAIWNDQGEQV
                     IADRATYELLAGALPPPDVRRLRALLEEGVVALDLTQDDEQMPSCMRAMHVRTGAMAS
                     LLAPIRAAGQVAGVFAVSYMRPRSFGAGERRLLAALSQRASLALENAQLFSQAEERTR
                     EIERRRAVAEGLRDLLAVVNSERTLDQVLNYIVAQSSRVLGSDGSAIYEPAADDADTL
                     EVRASGGLQPDYATPRIPRDYSATGLAFTSRRPVATGDVLATLEPGYQERWELAAEER
                     GSHTLLLRVPKPGVVAGPGPASPLGAGAGYRTLLAVPLAVSAEAYGALTLFYRVPRTF
                     SNDELALATAFADQAALAIENARLRERARQAATLEERQRLARELHDAVTQTLFSASLI
                     AEVIPDLWDTKPEEARRRLEQLRRLTRGALAEMRSLLVELRPGGLSDLPLADLLGQLA
                     EATRGRTQVDIALQIEGRPAALPSDTQIALYRIAQEALNNVVRHAQASSAVLALRYAP
                     GPRVRLSVVDDGLGFDLAAIPAGHLGVGIMRERAAAIGATLRVTSSRGGGTRVEVDWQ
                     GEGAPG"
     gene            complement(3156..>4073)
                     /locus_tag="VFU72_13405"
     CDS             complement(3156..>4073)
                     /locus_tag="VFU72_13405"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424536.1"
                     /translation="LISWVAGCAQVFDTPARQALVVDSVPRELAPNAMALNALAQRLA
                     GAAGALGGGLLIPLLGLGNCYLVIAFGYGLTALLSATLPAPRRVQTTIAHAPFSHALR
                     DAARLIVHLPAVRTLTISGVACEIFAFSYSSALPIFTTDVLAIGAAGLGLLNAAASLG
                     GTLGLLLLSLVVGRLRREPLLAAVFLGYGASLVLLGMVRELWLAVAVLVLTGLCAAAF
                     DMLQQTLLQLAVPEEQRGRAVGIWVLGVGSAPVGNLEMGTLAGLLGAPGGLLVNGALT
                     IVSAALLLARAPHYRGSPRQRVEELPRLG"
BASE COUNT          592 a         1468 c         1460 g          553 t
ORIGIN      
        1 gccggcctgg agcgcatgct gcaccagatc tccttcgagg aaactggtca ggacgatcac
       61 ctgcgtctcc gggtgggcgg cgcggatcgc ccgggtggcc tccgggccgt cgatgcccgg
      121 catgaccata tccatcagca ccacgtccgg ccgcagctcg cggcagaggc ggatcgcctc
      181 ttcgccgctg ccggcctcgc cgacgagctc gagatccggc gcgaggccca ggaaggcggc
      241 cagtccgctg cgcaccatct cgtggtcgtc cacgatcagc acgcggatcg gccgcttgtg
      301 cgtctctgcc tggctcatcc cggcgcgccc tctccttgcc agtcaacctc gacgcgagtg
      361 cccccgcccc ggctgctggt gacgcgcagc gtggcgccga tggcggccgc gcgctcccgc
      421 atgatgccga cgccgaggtg accggccggg atcgccgcca gatcgaaccc cagaccgtcg
      481 tcgacgacgg agagccgcac gcgcgggccg ggcgcgtagc ggagggcgag cacggcggac
      541 gacgcctgcg cgtgcctgac gacgttgttg agcgcctcct gggcgatccg gtacagcgcg
      601 atctgcgtgt cggacgggag cgcggcgggc cgtccctcga tctggagggc gatgtcgacc
      661 tgcgtgcggc cgcgggtagc ctcggccaac tgccccagga gatcggcgag cggcagatcg
      721 gacagcccgc cggggcgcag ctccaccagc aggctgcgca tctccgccag cgcgccgcgc
      781 gtcagccggc ggagttgctc cagccgccgc cgcgcctcct ccggcttcgt atcccagaga
      841 tccgggatca cctccgcgat caggctcgcg gagaacagcg tctgcgtgac cgcgtcgtgc
      901 agctcgcgcg ccagccgctg gcgctcttcc agcgtagcgg cctgcctggc ccgctcgcgg
      961 agccgggcgt tttcgatcgc cagcgcggcc tggtcggcga acgcggtggc cagcgccagc
     1021 tcgtcattgc tgaacgtgcg cggcacgcgg tagaagagcg tcagcgcgcc gtaggcctcg
     1081 gcgctcaccg ctagcggcac ggccagcagc gtgcgatacc cggcgcccgc gccgagcggg
     1141 ctggcgggcc cgggaccggc gacgacgccc ggcttcggca cgcggaggag cagcgtatgg
     1201 ctaccccgct cctcggccgc cagttcccag cgctcctggt agccgggttc gagcgtggcg
     1261 agcacgtcgc ccgtcgccac cgggcggcgg ctggtgaacg ccaggccggt cgcggagtag
     1321 tcgcggggga tgcgcggcgt ggcgtagtcc ggctggagcc cgccgctggc acgcacctcc
     1381 agggtatccg cgtcgtcggc ggcgggctcg tagatggcgc tcccgtcact gccgagaacg
     1441 cggctggact gcgccacgat gtagttgagg acctggtcga gcgtgcgctc cgaattgacc
     1501 acggcgagca gatcacgcaa tccctcggcg accgcgcggc gccgctcgat ctcgcgggtc
     1561 cgctcctcgg cctggctgaa gagctgcgcg ttctcgagcg ccaggctggc gcgctggctc
     1621 agcgcggcga gcaagcgtcg ctccccggcc ccgaagctgc gtggccgcat gtagctcacc
     1681 gcgaacaccc cggccacctg gccagcggcg cggatcggcg ccagcagcga tgccatggcg
     1741 cccgtgcgca cgtgcattgc gcgcatgcac gacggcatct gctcgtcgtc ctgggtgaga
     1801 tcgagcgcca ccactccttc ctcgaggagg gcacgcagac gtcggacgtc gggtgggggg
     1861 agcgctcccg cgaggagctc ataggttgcc cggtccgcga tcacctgctc gccctggtcg
     1921 ttccagatcg cgaccgcggc ggcatccgac gccaggatgc cgttcgcgac atccaccagc
     1981 gcgcgcagta cgtcctcgac ccgcagcgag cggtgcagcg cctcgtccgc gtcgtagagc
     2041 gcctccagct cgcggcggcg ttgctcctgc cgcgcgtgca actgcgcgtt ctcgatggcc
     2101 agcgccgctt gattggcgaa ggcggtggcc agtgccactt cttcatcgcc gaactcgtgc
     2161 ggccggctat agtagagggt cagcgcgccg tagctctcct ccttgaccac cagcggtacg
     2221 gccagcagtg cgcggtagcc tcccgcgtat gctccgtaga ccggcgcgcg gggatgatcc
     2281 accggcagca gcgagacggg gaggtggatg atgcgcgcgt agctcgcgcg ctcctccagc
     2341 tccaggccgc gggtcaggtc gcggtcttcc ggcggaatcg gctggacggc ggtattggcg
     2401 aagggccggc gcaggctgaa cgccagcccg gtcgtcatca tcccaatcgg gatcgtgcgg
     2461 gccgggtagg ccggatcgag cccgctctcc gctcgcacct tcaggcgccg cccgctggcg
     2521 tccggggtga agatcgcgct ggcgtcgctg cccagcagac gccgcccctg ctcgacgatg
     2581 tagtcgagga tctcgtcgag ggtgcggccg gaattgacca cggccagcag atcgcccagc
     2641 gcctgggata ccgcgtgccg ctgctcgatc tcgcgcgtcc gctcggcgac acgccgctcc
     2701 agctcctcgt attggcgcac ctgctcggtg acgtcgcgca cgacgccgag catcgccggc
     2761 gcgccgtgat agacgatgcc gcgaccgagg acttcggtat cgaagacgct gccgtcgcgg
     2821 cggacatcgc gcgactggca gcagaactcg cggccctcgc gaatcgcccg ggcgaactcc
     2881 cgcaggagat gacgggagtc gggatggatg aaggtggacg ggtacatacc gaccatctgc
     2941 tcgtagccgt gcatacggca gaaggccggg ttggcggcca gcacgacgcc ggtcgagagg
     3001 tcgttgacga ccagcccgtc gctgctggct tcgaagatgg cttgatacgg ctgctccgtg
     3061 ccgtggagcg tgtccatcaa atcctcgatt ggcgcgcgca tttccacaat ttacgatgaa
     3121 cctattctaa ccggcggcgc gggcgccgtc tacggtcagc ccaagcgcgg cagctcctcg
     3181 acccgctgtc ggggcgagcc gcgatagtgc ggggcgcggg cgaggaggag cgcggccgag
     3241 acgatggtca gcgcgccgtt gaccagtagc cctcccggcg cgcccagcag gccggccagc
     3301 gtgcccatct ccaggttgcc gaccggcgcc gagccgaccc ccagcaccca gataccgacc
     3361 gcgcgcccgc gctgctcctc cggcaccgcc agctgtagga gggtctgctg cagcatgtcg
     3421 aaggcggcgg cgcacaggcc ggtcagcacc agcaccgcga ctgccagcca cagctcgcgc
     3481 accatgccaa gcagcaccag cgaggcgccg tagccgagga agacggccgc cagcagcggc
     3541 tcgcgccgta gccgccccac caccagcgag agcagcagca gccccagcgt gccgccgagc
     3601 gaggcggcgg cattcagcag ccccagccca gccgcgccga tggccagcac atccgtggtg
     3661 aagatcggca gcgcgctgga gtaggagaag gcgaagatct cgcaggcgac gcccgagatc
     3721 gtcagcgtgc gcaccgccgg caggtgcacg atcaggcggg cggcgtcgcg cagcgcgtgg
     3781 ctgaagggcg cgtgggcgat tgttgtctgg acgcggcgcg gcgcggggag cgtggcggac
     3841 agcagcgcgg tcagcccgta gccgaaggcg atcaccaggt agcagttgcc cagcccgagc
     3901 agcgggatca gcagcccgcc gcccagggcg ccggccgccc ccgccagccg ctgggccagc
     3961 gcgttgagcg ccatggcgtt gggcgccagc tcgcgcggga cgctgtcgac caccagcgcc
     4021 tggcgtgccg gggtgtcgaa gacctgggcg cagccagcca cccacgagat cag
//
LOCUS       DASUBI010000691         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_250597, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000691 DASUBI010000000
VERSION     DASUBI010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250597"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU72_13410"
     CDS             <1..198
                     /locus_tag="VFU72_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424537.1"
                     /translation="REVVGPAMGRTAEEMLAAGLFGPAERCAARLRAYRAAGLQLALI
                     WPAADPLAQLQRFAEEVMPLV"
     gene            complement(392..1354)
                     /gene="argF"
                     /locus_tag="VFU72_13415"
     CDS             complement(392..1354)
                     /gene="argF"
                     /locus_tag="VFU72_13415"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5424538.1"
                     /translation="MPTPTKTAKFDLLTDDDLTPFQAAALIERAAELKAQHQAGEPQD
                     HLLRGKTLAMIFQKPSTRTRVAFEAGMAQLGGHALYLSTNDLQLGRGETISDTAAVLS
                     RYVDAIMARVYKHSDIVGLAAAASVPVINGLSDFAHPTQGLADMLTIKEHLGGWQGRK
                     VAYVGNTNNMLHSLLLAGSLVGLHLSVATPAHCTPQSEVVARAQALAAQTGATIELLA
                     NPYEAAYAADAIYTDVWVSMGQEIDAATMTELHAYQVNDELMSAASHNAIFMHCLPMY
                     RGQEATAEIADGPQSVIFDQAENRLHLHKALLVELLAPSGGLFD"
     gene            complement(1622..2242)
                     /locus_tag="VFU72_13420"
     CDS             complement(1622..2242)
                     /locus_tag="VFU72_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424539.1"
                     /translation="MSTSSEGELTFDDLTTVAGLVAGFPAPWYVSGGWAIDLFLGTTT
                     RAHEDLEIGVARADQALLHRQLGDRDLRKIVPRGETFDLLPWSEGEWLELPIHQIIVP
                     QAPDHPVEFQFFLNEVWSGEWRFRPDQTIRRPVEECYDVSPFGIPALAPEIQLLYKCR
                     RHGPKDEHDFRVVLPHLDASRRAWLSDALRRFRPDDPWLRELETSR"
     gene            2509..3660
                     /locus_tag="VFU72_13425"
     CDS             2509..3660
                     /locus_tag="VFU72_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5424540.1"
                     /translation="MDYPRRLAAFRERMGEAGIDLVYLPRGANLFYLTGVRRHYDHGT
                     DHNAYGDWATGAYLGRRGGLTLLGPRMGGTFYLDEAEGKPWIEAVRLIQEAEQPLEVM
                     RQTVAAFEPVRSLALDERTWARTDEALRGLLPEARVSLVSDLINPLRMIKDDEELAAM
                     RAASALADKVFALVAPQLRLGASELEITQEVDYQFLLNGAESTSFQTGVYIIGPEQSG
                     AWPNESRGGGRRLRPGDSLMFDFGCVLDGYCADFGRCAYAGEPSAEYLRVHELVLQSQ
                     QAGIDALRAGERTTAEVNAIARRVLEEAGYGDAFTHRLGHGIGVTVHEPPFLDVTDQT
                     ILRPNMVFTVEPSVLLRGKFGNRVEDVVVVTEHGGERLNSAPHALTVIQ"
     gene            3673..4665
                     /locus_tag="VFU72_13430"
     CDS             3673..4665
                     /locus_tag="VFU72_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424541.1"
                     /translation="MARHPWVAAADQQGVRFGPQIGAIRRRPVLPRWELYDKPMEILL
                     ETGRLVERLGFDAVYLHDHPLNSPEPWLGLAALAAVTERVMLGSVVNCVFYRHPAYLA
                     RMAADLDQLSHGRLMLGLGVGWHHGEFAAYQQPFDSPGKRLAALEEAIAIIEGSWGPE
                     RFSYQGKHFSTVEMAIVPGPLQQPRPPLMIAGAGEQVTLRQVARLADACNLDESKLPN
                     GPADIRRKLEVLQEHCEAVGRDYDEILRTHFVGWLMLAPTEAEVRRKVARYYPEGVPP
                     QFGRFVHAGTPAQFVEFYQARVEAGIQYFVPQLLDGTDHETLHLLAEEVMPQVG"
BASE COUNT          757 a         1617 c         1731 g          732 t
ORIGIN      
        1 cgcgaggtcg tcgggccggc gatgggccgc acggcggagg agatgctggc cgcgggactc
       61 ttcggcccgg cggagcgctg cgccgcgcgc ctgcgcgcct accgcgcggc gggactgcag
      121 ctcgcgctga tctggcccgc cgccgacccg ctggcgcagc tccagcgctt cgccgaggag
      181 gtcatgccac tcgtctgaga gcgccgaccg ataggctttg gtgtcgtcca cgttggaccc
      241 ctacgacaag acgtcatcga caggcagcgc ccctcgccca ccgcagtggg agaggggaag
      301 ggggtgaggg ccgtccgcca ggcacgcgat gcccgcgtgc gggcaccgcg tccctccctt
      361 gaaacccgaa gagtgcgtga aagattgaaa cctaatcgaa gagcccgccg ctgggcgcca
      421 gcaactccac cagcagcgcc ttgtgcaggt gcagccggtt ctcggcctgg tcgaagatga
      481 ccgactgcgg accgtcggcg atctcggcgg tcgcctcttg cccccggtac atcggtaggc
      541 agtgcatgaa gatggcgttg tggctggcgg ccgacatcag ctcgtcgttg acctgatagg
      601 cgtgcagctc ggtcatggtg gcggcgtcga tctcctggcc catgctgacc cagacgtcgg
      661 tgtagatcgc gtcggcggcg taggccgcct cgtaggggtt ggccagcagc tcgatggtgg
      721 cgccggtctg ggcggcgagc gcctgggcgc gggccacgac ctccgactgc ggcgtgcagt
      781 gcgccggggt ggcgaccgag aggtgcagcc cgaccagcga gccggccaag agcagcgaat
      841 ggagcatgtt gttggtattg ccgacgtaag ccaccttgcg cccctgccag ccgcccaggt
      901 gctccttgat ggtcagcatg tcggccagcc cctgcgtcgg gtgggcgaag tcggagagcc
      961 cgttgatcac cggcacgctg gcggccgcag ccagcccgac gatgtcgctg tgcttgtaga
     1021 cgcgggccat gatggcgtcg acgtagcgcg agaggacggc ggcggtgtcg gagatggtct
     1081 cgccgcggcc gagctgcaga tcattggtcg agaggtagag cgcgtggccg ccgagctggg
     1141 ccatgccggc ctcgaaggcg acgcgggtgc gggtggatgg tttctggaag atcatggcga
     1201 gggtcttgcc gcgcagcagg tggtcctgcg gctcccccgc ctggtgctgc gccttcagct
     1261 ccgcggcgcg ctcgatcagc gcggcggcct gaaagggcgt cagatcgtcg tcagtcagca
     1321 ggtcgaattt ggcggtcttg gtcggcgtag gcatgatcgg tcctcctgtc ggcacggtcc
     1381 cagcaagcga caacgcgaag cgcgaagctg gtcctgcgcc tgagccggat ggatccggac
     1441 acgtccacgt cccttctccc agtcaaagca acggagaaca caaccaaaca ccgtcgcgat
     1501 agcattccgc gacccacata cgatgcccga aatcgccgcg cctgtcaagt atttgaagga
     1561 ttctaacccc cagttggggg caagcagccg ctgtgtcggc caggtatcgc ggcgctgcgc
     1621 ctcagcggct cgtctcgagc tcgcgcagcc agggatcgtc gggccggaac cggcgcagcg
     1681 catcactgag ccaggctcga cgcgaggcgt cgagatgcgg caggacgaca cgaaagtcgt
     1741 gctcgtcctt cggcccgtga cgccggcact tgtagagcaa ctggatctcc ggcgcgagcg
     1801 ccggtatgcc aaagggagac acgtcgtagc actcttcgac cggccgccgg atggtctgat
     1861 ccggccggaa acgccactcg ccggaccaga cctcgttgag gaagaactga aactcgaccg
     1921 ggtggtcggg cgcctgggga acgatgatct ggtgaatggg cagctcgagc cactcccctt
     1981 cgctccaggg cagcaggtca aacgtctctc cccgaggaac gatcttgcgg aggtcccgat
     2041 cgcccagctg gcggtgaagc agcgcctgat cggcgcgcgc gacgccgatc tccaggtcct
     2101 cgtgcgctcg cgtggtggtg ccgaggaaga ggtcgatagc ccagccgccc gagacatacc
     2161 agggcgcggg aaaccccgcc accaagccgg cgaccgtggt cagatcgtcg aaggtgagct
     2221 cgccctcgga gctggttgac atcgcctgcg ccctcgttcg atcggccacg attcgcattc
     2281 gccggcaatc tacgccgggg cagcctagcc gggaaggcgg tcggcgtcaa cggccggcgc
     2341 tagcggggct agcggggaca gcttctaccg tttagggtcg accatgtcgc gctctggcat
     2401 caccctgcga tgctcgccac ggtcgggcgc tagactgttt cgctccccct cgaacagtgc
     2461 gatgatgccc gcacgatcaa gcacgagcga tgcgtttggg aggcggcgat ggactatccg
     2521 cggcggctgg cggcgttccg ggagcgcatg ggcgaggcgg gcatcgacct cgtctacctc
     2581 ccgcgcggcg ccaacctctt ctacttaacc ggcgtgcggc gccactacga ccacggcacc
     2641 gaccacaacg cctacggcga ctgggctacc ggcgcctacc tcggccggcg cggcgggctg
     2701 acgctccttg gcccgcgcat gggcggcacc ttctacctcg acgaggccga gggcaagccc
     2761 tggatcgagg cggtgcgcct gatccaggag gccgagcagc cgctggaggt gatgcgccag
     2821 acggtggctg ccttcgagcc ggtgcggagt ctcgcgctcg acgagcgcac ctgggcgcgc
     2881 accgacgagg cgctgcgcgg gctgctaccg gaggcgcgcg tctcgctggt atccgatttg
     2941 atcaacccgc tgcgcatgat caaggacgac gaggagctgg cggcgatgcg cgccgcctcg
     3001 gcactggccg acaaggtctt cgcgctggtc gcgccgcagc tccggctcgg cgccagcgag
     3061 ctggagatca cccaggaggt cgactaccag ttcctgctca acggcgccga gagcacctcg
     3121 ttccagaccg gtgtctacat catcgggccg gagcagagcg gcgcctggcc gaacgagtcg
     3181 cgcggcggcg ggcggcggct acgccccggc gactcgctga tgttcgactt cggctgcgtg
     3241 ctcgacggct actgcgccga cttcggccgc tgcgcctacg ccggcgagcc gagcgccgaa
     3301 tatctacggg tgcacgagct ggtgctccag tcgcagcagg cgggcatcga cgccctgcgc
     3361 gccggcgagc gcaccacggc cgaggtcaac gccatcgccc ggcgcgtgct ggaggaggcc
     3421 ggctacggcg acgccttcac ccaccggctg ggccacggca tcggcgtgac ggtgcacgag
     3481 ccgcccttcc tcgacgtgac ggaccagacg atcctgcggc cgaacatggt cttcacggtc
     3541 gagccgagcg tgctgctgcg cggaaagttc ggcaaccggg tggaggacgt ggtggtggtg
     3601 accgagcacg gcggcgagcg gctcaacagc gcgccgcacg cgctgacggt cattcaatag
     3661 gaggaggcga gcatggcgcg acatccgtgg gtggcggcgg ccgatcagca gggggtgcgc
     3721 ttcgggccgc agatcggcgc gatccgccgc cggccggtgc tgccgcgctg ggagctctac
     3781 gacaagccga tggagatcct gctggagacc gggcggctgg tggagcgcct cggcttcgac
     3841 gccgtctacc tgcacgacca cccgctcaac agcccggagc cctggctggg gctggcggcg
     3901 ctggcggccg tgaccgagcg cgtcatgctc ggctcggtgg tcaactgcgt cttctaccgc
     3961 caccccgcct atctggcgcg catggccgcc gatctcgacc agctcagcca cgggcggctg
     4021 atgctcgggc tgggcgtcgg ctggcaccac ggcgagttcg ccgcctatca gcagcccttc
     4081 gactcgcccg gcaagcggct ggcggcgctg gaggaggcga tcgcgatcat cgaggggagc
     4141 tgggggccgg agcgcttcag ctaccagggc aagcacttca gcaccgtcga gatggcgatc
     4201 gtgccggggc cgctgcagca gccgcgcccg ccgctgatga tcgccggcgc cggcgagcag
     4261 gtgacgctgc gccaggtggc gcggctggcc gacgcctgca acctggacga gtcgaagctg
     4321 cccaacggcc cggccgacat ccgccgcaag ctggaggtgc tgcaagagca ctgcgaggcg
     4381 gtcgggcgcg actacgacga gatcctgcgc acccacttcg tcggctggct gatgctggcg
     4441 ccgaccgagg cggaggtgcg gcgcaaggtg gcgcgctact acccggaggg cgtgccgccg
     4501 cagttcggac gcttcgtgca cgccggcacc ccggcgcagt tcgtggagtt ctaccaggcg
     4561 cgcgtcgagg ccggcatcca gtacttcgtg ccccagctgc tcgacggcac cgaccacgag
     4621 acgctgcacc tgctggccga ggaggtcatg ccgcaggtgg gctgaggtaa gcctgagcgt
     4681 tccattcggt aggggcagac ccctgtgtct gccgatacgc atcaagttaa ggatttcgcg
     4741 atccagacgc gggttttggc gtcgtggtgc agcgatcggc ggatcgaccg aacccgaatc
     4801 gaccggccgt tcggtctctc tagcctccaa cgccacg
//
LOCUS       DASUBI010000692         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1309328, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000692 DASUBI010000000
VERSION     DASUBI010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309328"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFU72_13435"
     CDS             <1..895
                     /locus_tag="VFU72_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU5424542.1"
                     /translation="ERLRAGLIDFNAELTVVTETPATIAFDAHNGMGHPASYHAMTRC
                     IEKAKESGLCMATVRHSNHFGIAGYYAMMALKEGMCGLAMTNATPLVVPPFAREPVMS
                     TAPIAVAIPARDQRPIVIDMATSSVAWGKVEIARRENHPIPEGWAYDAEGKPTTDPFA
                     AVSLAPLGSTRDLGSHKGFGFSLFVEVLCAQLAGSSWSRYIAGSRVTPPRPSDTGHAF
                     MAWRIDAFRPLAEFLGGMDAMLGELRAAEPAPGHERVYVPGDPERIAEADRLERGVPV
                     NPKVLDDLRQIGQEVGVRAPF"
     gene            959..1294
                     /locus_tag="VFU72_13440"
     CDS             959..1294
                     /locus_tag="VFU72_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5424543.1"
                     /translation="MAAIDLARDYIWKVNARDGAAAAALFAPDGEIVDPRGGTHHGHE
                     AIAAFVNAATLGTLAQIGERHMGTGHALLRGVVITAGLPPAEVEWQFETAGDQITRLT
                     IRLPKPDEG"
     gene            complement(1495..1992)
                     /locus_tag="VFU72_13445"
     CDS             complement(1495..1992)
                     /locus_tag="VFU72_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5424544.1"
                     /translation="MTIDTTNQTGLAAPPKRGEQIPAFSGTTADGRALGTRYYYMRRN
                     LALVFTHGPGCAGCETLLRELGELEEAVQAEAGETLAVVPAAGEELRGLAAELPFPVV
                     GDPDLAIHRRYGLVDAQGAPRAAIFVADRYGVVFEPSLADDEHRMLEAAEVPGWLEFI
                     ACRCT"
BASE COUNT          351 a          811 c          789 g          344 t
ORIGIN      
        1 cgagcgcctg cgggccggtc tgatcgactt caatgctgag ctgacagtcg tgaccgagac
       61 gccggccaca atcgccttcg atgcccacaa cggcatgggc cacccagcct cctatcacgc
      121 catgacgcgc tgcatcgaaa aggcgaagga aagcggcctc tgtatggcga cggtgcgcca
      181 tagcaaccat ttcggcatcg ccggctatta cgccatgatg gcgctcaaag aggggatgtg
      241 cggtctggcg atgaccaatg ccacgccact tgtcgtgccg cccttcgcgc gtgagccggt
      301 gatgagcacc gcgccgatcg ccgtcgccat tccggcgcgc gaccagcgcc cgatcgtgat
      361 cgacatggcg acgagttcgg tcgcctgggg caaggtcgag atcgcccgac gggaaaacca
      421 cccgattccc gaaggctggg cctacgacgc cgaaggcaag ccgacgaccg atccgttcgc
      481 ggccgtctcg ttggcgccgc tcggctcgac gcgtgatctc ggcagccaca agggcttcgg
      541 cttctcactc ttcgttgagg tgctctgtgc ccagttggcc ggctcatcct ggagccgcta
      601 catcgccggc tcacgcgtca cgccgccgcg gccctccgat accggccacg ccttcatggc
      661 ctggcgcatc gacgccttcc gcccgctggc cgagtttctg ggcggcatgg acgccatgct
      721 cggcgagctg cgcgcggccg agccggcccc cggccacgag cgcgtctacg tgcccggcga
      781 cccggagcgc atcgccgagg ccgaccggct ggagcgcggc gtgccggtca accccaaggt
      841 gctggacgat ctgcgccaga tcgggcagga ggtcggcgtc cgggcgccct tctaagcgcc
      901 gctggggtag ctggcacggc ccgtgcgccg cgcgacggcg aaacgtgagg ggagcgcaat
      961 ggcggcgatc gatctggcgc gcgactacat ctggaaggtc aacgcccgcg acggggcggc
     1021 ggccgcggcg ctcttcgcgc ccgacggcga gatcgtcgac ccgcgcggcg gcacgcatca
     1081 cgggcacgag gccatcgccg ccttcgtcaa cgcggccacg ctcggcacgc tggcgcagat
     1141 cggggagcgg cacatgggca ccggccacgc gctcttgcgc ggtgtggtca tcaccgcggg
     1201 cctgccgccc gccgaggtcg agtggcagtt cgagaccgct ggcgaccaga tcacccgcct
     1261 gacgatccgc ctgccgaagc cggacgaggg gtgaggccgg cgctgcctgt cgaatgactc
     1321 gggtggctgg tgaggacgga accttcgaca agctcaggat gacaggggta cgcgagaggc
     1381 tgacgaggga gatgtgcctc gtccgcaagg cctcaatcca tagactgtca tcctgagctt
     1441 gtcgaaggat ccgtcctcgc tggcttccag tcttctcgaa tgccgcccag cccactacgt
     1501 gcagcggcag gcgatgaact ccagccagcc gggcacctcg gcggcctcca gcatccggtg
     1561 ctcgtcgtcg gccagcgacg gctcgaagac cacgccgtag cggtcggcca cgaagatggc
     1621 ggcgcgcggc gcgccctgcg cgtcgaccag cccgtagcgg cggtgaatgg cgaggtccgg
     1681 gtcgccaacc accgggaagg gcaactcggc cgccaggccg cgtaattcct cccccgcggc
     1741 cgggacgacc gccagcgtct cgcccgcctc cgcctgcacc gcctcctcca gctcccccag
     1801 ctcgcgcagc agcgtttcgc agcccgcaca cccgggtccg tgcgtgaaga ccagcgccag
     1861 attgcggcgc atgtaatagt agcgcgtgcc gagtgcgcgg ccatccgcgg tcgtaccgga
     1921 gaaggcgggg atctgctcgc cccgcttcgg cggggcagcc agtccggtct ggttggtcgt
     1981 gtcgatggtc ataatggcaa cgctcctcac tgttcatcag cataccggcg cgggcgcccg
     2041 gcctcatgct accagcgcac gcggttcgcc accgaaacgc ggttcgttgt gcggagattc
     2101 ccctgcgagc atgcgacggc gtgcctggac ggggggtctc agacggctgc gtcgcgcccg
     2161 cgcgcagtca gttcagccag cagatcgtcg agcggctggc gctgccctgc ttcataggcg
     2221 gtggcgaacg cgttttcgcc gagcgcggtc tgagctgcca gcagcgctcg ctggtagccg
     2281 gccgggtggg ccgcg
//
LOCUS       DASUBI010000693         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_264345, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000693 DASUBI010000000
VERSION     DASUBI010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264345"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="malQ"
                     /locus_tag="VFU72_13450"
     CDS             <1..1407
                     /gene="malQ"
                     /locus_tag="VFU72_13450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU5424545.1"
                     /translation="FVDFLVEAGQGLWQILPLNPTGSDGSPYNARSALAGNPLLIDLE
                     QLVEQGLLPYGELPRYGDDQTATVDFVEAAAGAEAALRAAFARFRQTEPAGLAARYAV
                     FRRAQYRWLDDYVLFAALHRAHNGAPWYTWGNDLARRDPATLDLWRRRLQDEVDYRAF
                     VQFLFDDQWLAVRRYANERGVRVIGDIPIYVAFDSADVWANQSLFALTPEGQPAVVAG
                     VPPDLFSATGQLWGNPCYRWDRLAAQGYQWWVARFRRALQHCDLVRLDHFRGFAAGWQ
                     VPAGEETAIGGSWVPGPGRAFFARLAAELGPLPVIVEDLGVITEDVDELRHELGYPGM
                     KVLQFAFDGKSDNPYLPHNYEPNCVVYTGTHDNDTTMSWYQSLDSVTRTQVDRYLGPH
                     DPDAAWALLRLAQASVARYAIAPLQDVLSLGGEARMNTPGTAEGNWRWRFREEDLAPG
                     LAHRLAELSAAYGRVPGA"
     gene            complement(1423..1842)
                     /locus_tag="VFU72_13455"
     CDS             complement(1423..1842)
                     /locus_tag="VFU72_13455"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEU5424546.1"
                     /translation="MSNSKIFLRGALRRCPNCGERDITTNWWTLRDRCPNCGLFFERE
                     EGYWTGAIAINLVITEIFFMLLVVGVVVATWPDIPMFKLLAGAIAVNVIVPLIFFPIG
                     KTVWMALDLVLHPLEPVESHEVERLQAERRQHQSLVK"
     gene            1942..3303
                     /locus_tag="VFU72_13460"
     CDS             1942..3303
                     /locus_tag="VFU72_13460"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5424547.1"
                     /translation="MDYRTDEIALATDLYEYTMGASYLALGMDQEAIFSLFVRQLPTS
                     RAYLVAAGLAEALDRVTRLRFDPSTLDYLRSTGQIRSELLDWLRDFRFTGDVRAVPEG
                     RIVFAGEPLVEVRAPITQAQLVETLLLNALHYPTLVASKAARYVSVAGGRALVEFGLR
                     RTPSIDAGLAAARAAYLAGFVGTSNLLAGARYGIPVSGTMAHSFVQVFPSERAAFLAF
                     AETFPGPVTLLIDTYDTLQGARHAAEVAQELAPQQRTIAAVRIDSGDFLALSRAVRAI
                     LDEAGFPEIQIVATGGLDEQHLAELERAGAPIDAFGIGTRLGVVADTPSLEMVYKLVA
                     YAGHGTAKLSQGKATIAGPKQVWRRHGADGLYVEDMLATASEPAPGTAWEPLLELVVR
                     GGKALAAPSLVDSRRRHREEMARLPEWLKQLEPERAFPVVLSSRLRAEHEATIAAIQR
                     REE"
     gene            3376..3720
                     /locus_tag="VFU72_13465"
     CDS             3376..3720
                     /locus_tag="VFU72_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5424548.1"
                     /translation="MIQAQGERPVVPEISVQEAEQRARSGTGVIVDVREPEELEEAAV
                     PGALHIPLGQLAQRAGELPQNKELLLFCRSGNRSSFATEYLRGAGRDDAVNVAGGILA
                     WAEAGLPIRFGS"
BASE COUNT          615 a         1261 c         1397 g          654 t
ORIGIN      
        1 ttcgtcgact tcctggtcga ggccggacag ggtctctggc agatcctgcc gctgaacccg
       61 accggatcag acggatcgcc ctacaacgcg cgctctgccc tggccgggaa tccgctcctg
      121 atcgacctgg agcagctggt cgagcaaggg ctgctgccct acggcgagct tccccggtat
      181 ggcgatgacc agacagccac agtcgacttc gtcgaggcgg cggcgggcgc cgaagcggcg
      241 ctgcgcgcgg ccttcgcgcg cttccggcag acggagccgg cgggcctcgc agcgcgttac
      301 gccgtctttc gccgggcgca gtaccgctgg ctggacgact acgtgctctt cgcggcgctg
      361 caccgggcgc acaacggcgc gccctggtac acctggggaa acgacctggc gcgccgcgat
      421 ccggctacgc tggatctctg gcggcgccgg cttcaggatg aggtcgacta tcgggcgttc
      481 gtgcagttcc tctttgacga ccagtggctg gccgtacgcc gctatgccaa cgagcgcggc
      541 gtgcgcgtca tcggcgacat ccccatctac gtggcgttcg atagcgccga tgtctgggcc
      601 aaccaatcgc tcttcgcgtt gacgcccgag gggcaacccg ccgtggtggc cggcgtgccg
      661 cccgacctct tctcggcgac cggtcaactg tgggggaacc cctgctaccg ttgggacagg
      721 ctggccgcgc agggctacca gtggtgggtg gcgcgctttc ggcgcgcgct ccagcactgc
      781 gacctggtgc ggctggatca ctttcgcggc ttcgcggcgg gttggcaggt gccggccggc
      841 gaagagacgg cgatcggcgg aagctgggtt cctggccctg gccgggcgtt cttcgcgcgg
      901 ctggcggctg agctcggacc gttgccggtc atcgtcgaag acctcggcgt catcaccgag
      961 gacgtcgatg agctgcggca cgagctgggc tatcctggta tgaaggtgct gcagttcgcc
     1021 ttcgacggca agagcgacaa tccgtacctg ccgcacaact acgagccgaa ctgcgtggtc
     1081 tacaccggca cccacgacaa cgacaccacg atgtcgtggt accaatcgct ggattcggtg
     1141 acgcgcacgc aggtcgaccg ttatctcggg ccacacgacc cggatgccgc gtgggcgctg
     1201 ttacggctgg cgcaggcgag cgtcgcgcgc tacgcgatcg cgccgctgca agacgtgctc
     1261 tcgctcggcg gtgaggcgcg catgaacacg cccggcacgg cggaggggaa ctggcgctgg
     1321 cggtttcgcg aagaggatct tgctcccggg ctggcgcacc ggctggcgga gctcagcgcg
     1381 gcgtacgggc gcgtccccgg cgcgtgacac cgcccgggag gcttacttga ccagcgactg
     1441 gtgctgtctt cgctccgcct gcagccgctc gacctcgtgc gactccaccg gctccaacgg
     1501 atgcagcacc aggtcgagcg ccatccagac cgttttgccg atcggaaaga agatcaacgg
     1561 cacgatgacg ttgacggcga tcgccccggc gagcagcttg aacatcggaa tgtcgggcca
     1621 ggtcgcgacg acgacgccga cgaccagcag catgaagaag atctcggtga tgaccagatt
     1681 gatcgcgatc gcgccggtcc agtagccctc ttctcgctca aagaagagac cacagttggg
     1741 gcagcggtcg cgcagggtcc accagttggt ggtgatgtcg cgctcgccgc agttcggaca
     1801 gcggcgcagc gcgccgcgga gaaatatctt cgaattgctc atcgttcgcc tcccggcgcg
     1861 cccccgcgcg cggcataagg atagcaagga gcgttgccgg gtacgacgtt tgccgctacg
     1921 ctcgtggcga cgggaggccg gatggattac cgcaccgacg aaatcgcgct ggcgaccgac
     1981 ctgtacgaat acaccatggg cgcgagctat ctggcgcttg gtatggacca ggaggcgatc
     2041 ttcagcctgt tcgtgcgcca gctcccaacc agtcgtgcct atctggtggc cgcggggctg
     2101 gctgaggcgc tcgatcgcgt gacccggctc cggttcgatc cgagcacgct cgactacctg
     2161 cgctcgaccg ggcagatccg aagcgagctt ctcgactggc tgcgagattt ccgcttcacg
     2221 ggcgacgtgc gcgcggtacc tgagggccgc atcgtcttcg ccggtgagcc gctcgtcgag
     2281 gtgcgggcgc ccatcacgca ggcacagctc gtcgagacgt tgctgttgaa cgcgctgcac
     2341 tatccaacgc tcgtcgcaag caaggccgca cgctacgtga gcgtggccgg cggcagagcg
     2401 ctggtggagt tcggtctgcg acggacgccg agcatcgacg ccgggttggc cgccgcgcga
     2461 gccgcctatc tggctggctt cgtcggcacg agcaatctgc tggccggcgc gcgctacggc
     2521 atcccggtca gcggcacgat ggcgcactcg tttgtccagg tcttccccag cgagcgcgcg
     2581 gccttcctgg ccttcgccga gacttttccg gggccggtga cgctcctgat tgacacctac
     2641 gacacgctgc aaggcgcgcg ccatgcggcg gaggtggcac aagagctggc gccgcagcag
     2701 cggacgattg ccgcggtgcg gatcgacagt ggagacttcc tggcgttgag ccgcgccgtg
     2761 cgcgcgatcc tcgacgaggc tggtttcccc gagatccaga ttgtcgccac tggtgggctg
     2821 gatgagcagc acctggcgga gctggaacgc gcgggggcgc cgatcgacgc gttcggcatt
     2881 ggtacccggc ttggcgtggt ggctgacaca ccctcacttg agatggtcta caagctggtg
     2941 gcctacgcgg ggcacggcac cgccaagctg agccagggca aagcgaccat cgcgggaccg
     3001 aagcaggtct ggcggcggca tggcgcggat ggactctacg tggaagacat gcttgcgacc
     3061 gcgagcgagc cggcgcccgg taccgcatgg gagccgctgc tggaattggt cgtgcgtggg
     3121 ggtaaggcgc tggccgcccc cagcctggtg gattcacgcc ggcggcatcg cgaggagatg
     3181 gcgcggctgc ccgagtggct caagcagctt gagccggagc gtgcgtttcc ggttgtcctc
     3241 agtagccggc tgcgggccga gcacgaggcg actatcgccg cgatccaacg gcgggaggaa
     3301 tagcgtcggt cgcacccgcg ttgcgagccg agactgcccg acgagtttcg cccatcagtg
     3361 tgagagcgag gagtgatgat tcaggcgcag ggtgagcggc ccgtggtacc ggagatctcg
     3421 gtgcaagagg ccgagcagcg cgcgcggagc ggcacgggtg tgatcgtcga tgtgcgcgag
     3481 ccggaggagc tggaagaagc cgcggtgcct ggggcgctcc acataccact gggacagctc
     3541 gcgcagcgag ccggcgagct gccgcagaat aaggagctcc tcttgttctg ccgctccggg
     3601 aatcgcagct ctttcgcgac cgagtatcta cgcggggcgg gacgcgacga cgcggtcaac
     3661 gtggcgggcg ggattctggc ctgggcagaa gcggggctcc cgatccgctt tggctcgtga
     3721 gccagtcttc gttaccggcg cagtaccgtc agccagggtg cggcctagcc gtttgggcgg
     3781 gcggcgcccc ccgtcgcgag cgtataatcc acctgcgggt tcgcgttttg tggaatgaga
     3841 aacggcaatg gcagcacccg taggtcgaat ggctcgctgg ggacgcgatc gaggactggc
     3901 ggcgcgcatg ttcttcgtca tgttcct
//
LOCUS       DASUBI010000694         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_632101, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000694 DASUBI010000000
VERSION     DASUBI010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632101"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFU72_13470"
     CDS             <1..683
                     /locus_tag="VFU72_13470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU5424549.1"
                     /translation="PIRISAQVGVGVEPVQSIEREINLSGPIHSKGVLTLLGYLTGTY
                     AQDYPLAVTGALAFEQSYDEIEGDSASSAELYALLSALSGLALDQQIAVTGSVNQHGE
                     VQAVGGVTRKIEGFFAVCQARGLTGRQGVMIPAANLRNLMLAQEVVDAVRDGRFHIWA
                     VHRIVEGIELLTGTPAGERAADGSYPPGSVHQLVAERLRHYATRASLLRDLTHGNGLT
                     RPAPVSPS"
     gene            945..1628
                     /locus_tag="VFU72_13475"
     CDS             945..1628
                     /locus_tag="VFU72_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424550.1"
                     /translation="MNRRRLLTAGGGVLVAAMLGVGVVGTAFADNPATPTGSQPATSQ
                     ESRAQRFLDKLAANLGLSSDQLQNGLKTTEKQYVDQAAQNGKLTPEQATALEQQIDQS
                     NGIAPLGRFLQQRFQQAKVNIGKAVADTLGITPQQLKSELQSGKTLKDVIVEHSPGKT
                     PDQAVQDVVNAVVAQAKTRLDTAVRNGKLSSEQETTILNNLSQRLTNRINNPLRPGVQ
                     PAPNATPGA"
     gene            complement(1699..2487)
                     /locus_tag="VFU72_13480"
     CDS             complement(1699..2487)
                     /locus_tag="VFU72_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5424551.1"
                     /translation="MDLQLTGKRALVAAASKGLGKAVATELAREGARVAIFSRDAERI
                     QQTADEIRQATGAEVLPLVADARQPGDIERVVEQTVKQFGGLDILVTNAGGPPPGTFE
                     SFSAQDFLDAIELNLMSTIRMAKAAVPHMKEHGGSIVTITSISVKQPLENLILSNTSR
                     TGVVGLVKSMANELGKYNIRVNNVGPGPTRTDRILNLARRRAEEGGVPLEQALAESAT
                     GIPLGRVGEPEEFGRVVAFLASPAASYVTGSTLLVDGGLYAGSL"
     gene            2655..>3462
                     /locus_tag="VFU72_13485"
     CDS             2655..>3462
                     /locus_tag="VFU72_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5424552.1"
                     /translation="MSRGRVYRSNQRGPRGRRRNRRFGNRPPARRALPPSILSSHDRP
                     RRGFRALGLATQFAVAALLGTLILFVIAVGAVAAGSIAGWSYVTTDLPNLDQVEAPQF
                     QTTKIYDRNWKLLAEVVDPTTGYRTDVTFQEVEDHIAQQQDDPNAPHRAWIFDATVAA
                     EDATFWTNPGVDPVAIVRSAFLELAGGRTGASTITQQLVRNLYTEKIGNERTLTRKIR
                     EAVVAYQFSKKYKKSTVLEMYLNQVFYGHRSYGIDAASLTYFNKHPWNLTL"
BASE COUNT          564 a         1259 c         1116 g          523 t
ORIGIN      
        1 ggccgatccg tatctcggcc caggtgggcg tgggcgtcga gccggtgcag agtatcgagc
       61 gcgagatcaa tctctccggg ccgattcact ccaagggcgt gctgaccctg ctcggctacc
      121 tgaccggcac ctacgcccag gactaccccc tggcggtcac cggcgcgctc gctttcgaac
      181 agtcgtacga cgagatcgag ggcgactccg cctcgtcggc cgagctctac gcgctcctct
      241 cggcgctctc ggggctggcg ctcgaccagc agatcgcggt caccggctcg gtcaaccagc
      301 acggcgaggt gcaggcggtc ggtggcgtca cgcgcaagat cgagggcttc tttgccgtct
      361 gccaggcgcg cggactaacc ggccggcagg gcgtgatgat cccggcggcc aacctgcgca
      421 acctgatgct ggcgcaggag gtcgtcgatg cggtccgcga cggccgcttc cacatctggg
      481 cggtgcaccg gatcgtcgag gggatcgagc tgttgaccgg cacgccggcc ggggagcgtg
      541 ccgccgacgg ttcctatccg cccggcagcg tgcatcagtt ggtggccgag cgcctgcgcc
      601 actacgccac ccgagcgagc ctgctgcgtg acctcaccca cggcaacggc ctgacgcgcc
      661 cggccccggt cagtccgagc tagcgcgccg gccacgaatg tcggccccgc cacccgtgtg
      721 aggccgaact tcccgccctg tcatcctgag ctacggcgaa ggctctctcc tcaatctgga
      781 gcagcagcga tgttgcaagc gaggggagcg tcttcggctg cggcctcaga acgacatcgg
      841 ggggatgcgc tttacctgcg gttaacagag cgttgctacg ctccgtcttg aaggcttcgg
      901 aagggccgga gccgtgagac gacaacggtc gtatggagga agacgtgaat cgcagacgtt
      961 tgctgacagc gggcggcggt gtgttggtgg ccgcgatgct gggtgtcggc gtggtgggca
     1021 ccgccttcgc ggacaacccc gccaccccca ccgggagtca gccagcaaca agccaggaga
     1081 gccgcgccca acgattcctc gataagctgg cggccaatct cggcctcagc agcgatcagc
     1141 tccagaacgg cctgaagacg accgagaagc agtacgtcga tcaggccgcg cagaacggta
     1201 agctaacccc ggagcaggcg accgcgctcg agcagcagat cgaccagagc aacggtatcg
     1261 cgccgctcgg ccgctttctg cagcagcgct tccagcaggc gaaggtcaac atcggcaagg
     1321 cagtcgccga cacgctcggg atcaccccgc agcaactgaa gagcgagctg caatcgggca
     1381 agacgctcaa ggacgtgatc gtcgagcaca gccccggcaa gacgccggat caggcggtgc
     1441 aggatgtggt caacgccgtg gtggcgcagg ccaagacgcg gctcgacacg gcggtgcgga
     1501 acggcaagct gagttctgag caggagacga cgatcctcaa caacctcagc cagcggctga
     1561 ccaaccgcat caacaatccg ctgcgcccag gcgtccaacc ggcgccgaac gccacacccg
     1621 gcgcctgagc gccgctaccc acctttccct tccctttccc cacacggccc cgccccccaa
     1681 gcggggccgt ccgcctgcct acaagctccc cgcgtagagg ccgccgtcga ccagcagggt
     1741 cgagccggtg acgtagctgg cggcgggcga ggccaggaag gcgaccacgc gcccgaactc
     1801 ctccggctcg ccgacccggc ccagcgggat gcccgtggcg ctctcggcca gcgcctgctc
     1861 cagcggaacg ccgccttcct ccgcgcgccg tcgcgccagg ttgagaatgc ggtcggtgcg
     1921 cgtcgggccg gggccgacgt tgttgacccg aatgttgtac ttgcccagct cgttggccat
     1981 cgacttgacc agcccgacca cgccggtgcg tgaggtgttg gagaggatca ggttctccag
     2041 cggctgcttc accgagatcg aggtgatggt cacgatgctg ccgccgtgct ccttcatgtg
     2101 cggcaccgcc gccttggcca tgcggatggt gctcatcaga ttcagctcaa tggcgtcgag
     2161 gaagtcctgc gcgctgaagc tctcgaaggt gccggggggc ggcccgccgg cgttggtcac
     2221 caggatatcc agtccgccga actgcttgac cgtctgttcc accacgcgtt cgatgtcacc
     2281 cggctggcgc gcgtcggcca ccagcggcag cacctcggcg ccggtcgcct ggcggatctc
     2341 gtccgccgtc tgctgaatgc gctcggcgtc gcgcgagaag atcgcgaccc gcgctccctc
     2401 gcgcgccagc tccgtggcga ccgccttgcc cagccccttg ctcgccgcgg ccaccagcgc
     2461 tcgcttacct gtcagttgta gatccaccgc gcccgcctcc ttcgctgagc attgcgcgcc
     2521 gtcgtggcgg ccttcttgcg taccgccccg cctcgatcgg cggcagtgta gcacggaccc
     2581 tgcggccgcc cgcgcggctg cccgtggtat catcgcgccg gctgcgacag tttcacacat
     2641 gggggcgatt tgtcttgagc cggggcaggg tctatcggtc gaaccagcgc gggccgcgcg
     2701 ggcggcggcg caaccggcgc ttcggcaacc ggccgccggc ccggcgcgcg ctcccgcctt
     2761 ccatcctctc ctcccacgac aggccccggc gcggcttccg cgcgctcggg ctggcgaccc
     2821 agttcgcggt ggcggccctg ctcggcacgc tgatcctctt cgtcatcgcg gtcggcgcgg
     2881 tggcggcggg cagcatcgcg ggctggtcgt acgtcaccac cgacctgccc aatctcgacc
     2941 aggtggaggc gccccagttc cagacgacca agatctacga ccgcaactgg aagctcctgg
     3001 ccgaggtggt cgaccccacc accggctacc gcaccgacgt caccttccag gaggtcgaag
     3061 atcacatcgc ccagcagcag gacgatccca acgcgccgca ccgcgcctgg atcttcgacg
     3121 ccaccgtggc cgccgaggac gccaccttct ggaccaaccc cggcgtcgac ccggtcgcca
     3181 tcgtgcgcag cgcgttcctc gagctggccg gcggccgcac cggcgcctcg accatcacgc
     3241 agcagctcgt gcgcaacctc tacaccgaga agatcggcaa cgagcgcacc ctgacgcgca
     3301 agatccgtga ggcggtcgtc gcctaccagt tctccaagaa gtacaagaaa tcgaccgtgc
     3361 tggagatgta cctcaaccag gtcttctacg gccaccgctc ctacggcatc gacgccgcct
     3421 cgctgaccta cttcaacaag cacccctgga acctgaccct ca
//
LOCUS       DASUBI010000695         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1155391, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000695 DASUBI010000000
VERSION     DASUBI010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155391"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFU72_13490"
     CDS             <1..870
                     /locus_tag="VFU72_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5424553.1"
                     /translation="ASADAAPMYRVGHPRTRVRSVAAAPMLQGGEVVGLLSAQSYTPY
                     DYTSSDARLLMTIANHAVVAIEHARLYAQAQHLAVAEERNRLAREIHDTLAQGLIGIS
                     LYLEQVELALPEADAATRRLVERALALTRSNLEEARRSVKDLRAAQLEGRSLLEGIGL
                     LLAEQEADGAFRVELKAPLSLPPLEPPVETALYRVIQEALTNCRKHAQCQRVTVRLAL
                     DGERLLVTITDDGLGFDPERASAEPNRFGLASMRERIERVGGELWLESAPGRGTTVRA
                     AVPLCHALADTDD"
     gene            942..1880
                     /locus_tag="VFU72_13495"
     CDS             942..1880
                     /locus_tag="VFU72_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5424554.1"
                     /translation="MRKAHHVSTLAGVALLVISVVAFAWAAHGYLRGAASAPSPSTLP
                     ATPAATPSSAATPAPRPDLAGLTLSARAALLIDDAGTVLYAANPDEPLPPASTAKIVT
                     ALVVARYARPEEIVTIQPGDESDPSESTMGLRAGDTVTVHDLLVGMLLPSGDDAARVL
                     ARVVGERIPGDQPPRARFLAEMNALAAQLGMRDSHFTDPAGDDAPGQVVTARDLALAA
                     QRLLAEPSLLPIVATREARVRVGGPNARTLTLSNTNELLDTEGVFGVKTGTTPAAGQC
                     LVVAYQHAGRTEIAVVLGSQDRYADARALLGLPAAP"
     gene            1978..3630
                     /locus_tag="VFU72_13500"
     CDS             1978..3630
                     /locus_tag="VFU72_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5424555.1"
                     /translation="MGRDFNDPRGPSLEAPSRYGWRPDTSPEEPAEGASLAAVALAAT
                     AVLDAPVASSLDRAPDIPLWRPIARPLPRHRARRFPGKLPLHLLVLATIVAVVLGGGY
                     WSRAAIPAPAARPAGTSALRLGELPTTVGPTSDSGPSVLAAATSGGRPDVMTGYLSQF
                     SSAVIPNQLRVRTIVAEGSESIGDLATQTGLRIDTLLWANGLTDPTQELPAGTQVRVP
                     PVDGMLHIVHDGDTLESIAARYQVDVTAITGYGPNNVQTTADLVPYRLLMVPGGTMPT
                     RDKVETYVVRDGDTLATIAQFFGVHPNTILWANSLPDGDLIFPGQHLAILPADGVMVT
                     VKAGDTVESLAATYSVEPKAIRDYPGNGLGAAGTLRVDQLVMIPGGKPPKPPEPPPAP
                     APAAPAAPSVAQSQPAAAPAQHPASGRFIWPTSGTITQYYGPTSLWLEPAYLGYAHFH
                     QGLDIANGMYTPIVAAASGTVAFAGWTNVGYGYAVQIDHGNGLVTWYGHMAQMPAVHS
                     GQWVEQGQYLGPMGSTGASTGAHLHFGVLKNGVWDNPLNYLP"
     gene            complement(3810..4454)
                     /locus_tag="VFU72_13505"
     CDS             complement(3810..4454)
                     /locus_tag="VFU72_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5424556.1"
                     /translation="MLFEGETNLNLAQALFDLGGVEFGEFDLGPTAGRSPIYINPRVL
                     ISEPAVLRSIARLIAQEIQADQARRRPRLAGFAAVAGVPMGGLHLATAYALTSDTPLI
                     YVRPGGEQAQIEGKVIPGQTVLIIDDLMTGGRSILSTARTLEHNGVTVRDAIVLIDRE
                     QGGVERLHQHGYRVMPILRLRTMLTYYSESGLIDSTTYREAMAYLERTRPQHQH"
     gene            4561..>5822
                     /locus_tag="VFU72_13510"
     CDS             4561..>5822
                     /locus_tag="VFU72_13510"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU5424557.1"
                     /translation="MDYREAISYVIERSGYDKGFVANPFDAEMVGLRRTEWLLAALGH
                     PERRYPALHVAGTKGKGSTAATLAAIQRAAGRRVGLYTSPHLHTFRERIQLDGEPIAE
                     DDFATLVGEIAPLNARLAQERPDWGEATAFEVATVLALLAFARWEVDVAVVEVGLGGR
                     LDATNVLLPEVAVITAISLDHTRILGDTLAQIAAEKGGIIKPGRPVVSSPQRPEAAAT
                     LARIAAERGSPISFGGRDWQVASDSRSLALTGPWGRYEGLSLSLRGNYQLENAGTAVA
                     ACWALGQRGLPTPEAAIRAGLASVAWPGRFEIIHRQPIVVADGAHNVDSAERLAEALR
                     AEFASPSLTLILAIATDKDVEGMVQVLAPLAHTIIATASHNPRAASPERIAAAARAAG
                     LPPPRIVETPDVAGALRLALADAAPGDA"
BASE COUNT          830 a         2201 c         1973 g          818 t
ORIGIN      
        1 gcgtccgccg acgccgcgcc gatgtatcgc gtcggccacc cgcgcacgcg ggtgcgcagc
       61 gtggccgccg cgccgatgct gcagggcggc gaggtggtcg gcctactcag cgcgcagagc
      121 tacactccct acgattacac gagcagcgac gcgcgtctcc tgatgacgat cgcgaaccac
      181 gccgtggtgg cgatcgagca cgcgcgcctc tacgcccagg cgcagcacct ggcggtcgcc
      241 gaggagcgca accggctggc gcgcgagatc cacgacacgc tggcgcaggg cctgatcggc
      301 atcagcctct acctggagca ggtggagctg gcgctgcccg aggccgacgc cgccacccgg
      361 cggctggtgg agcgggcgct ggccctgacc cgcagcaacc tggaagaggc gcggcgctcg
      421 gtcaaggacc tgcgggccgc gcaacttgag gggcgcagcc tgctggaggg catcggcctg
      481 ttgctggccg agcaggaggc cgacggcgcg ttccgggtcg agctcaaggc gcccttgagc
      541 ctgccgccgc tcgagccgcc ggtcgagacc gccctctacc gcgtgatcca ggaggcgctg
      601 accaactgcc gcaagcacgc gcagtgtcag cgcgtcaccg tgcggctggc gctcgatggc
      661 gagcggctgc tggtcacgat caccgacgat gggctggggt tcgaccccga gcgcgccagc
      721 gccgagccga accgcttcgg gctcgcctcg atgcgcgagc ggatcgagcg cgtcggcggc
      781 gagctgtggc tggagagcgc gccgggtcgc ggcaccacgg tgcgggcggc cgtgcccctc
      841 tgtcacgccc tggctgacac cgacgactag ccgcgcggta caatgccccg gctgccattg
      901 attccgcgat tcagtcgctc acacgggagt agaccccgac cgtgcggaaa gcccaccacg
      961 tctcgacgct ggccggcgtc gcgctgctcg tcatcagcgt cgtggcgttc gcctgggccg
     1021 cccacggcta tctgcggggc gcggccagcg cgccctctcc ttccaccctg ccggccacac
     1081 cggcggcgac tcccagctcg gccgctaccc ccgcgccgcg ccccgacctc gccgggctga
     1141 ccctctcggc ccgcgccgcg ctcctgatcg acgatgccgg cacggtgctc tacgccgcca
     1201 accccgacga accgctgccg ccggccagca ccgccaagat cgtcaccgcc ctggtcgtcg
     1261 cgcgctacgc ccgccccgag gagatcgtca ccatccagcc gggcgacgag tcggacccct
     1321 cggagtcgac catgggcctg cgcgccggcg acaccgtcac cgtccacgac ctgctggtcg
     1381 gcatgctcct cccctccggc gacgacgccg cgcgggtact ggcgcgcgtc gtcggcgagc
     1441 gcatccccgg cgatcagccg ccgcgcgcgc gtttcctggc tgagatgaac gcgctggccg
     1501 cccagctcgg catgcgcgac agccacttca ccgaccccgc cggcgacgac gcgcccggcc
     1561 aggtcgtcac cgcgcgggac ctggcgctgg ccgcccagcg cctcctcgcc gagccgagcc
     1621 tcctgccgat cgtggccacg cgcgaggcgc gggtgcgtgt cggcggcccc aacgcgcgca
     1681 cgctgacact gagcaacacc aacgagctgc tcgacaccga gggcgtcttc ggcgtcaaga
     1741 ccggcaccac gcccgcggcg ggccagtgtc tggtggtcgc ctaccagcat gccgggcgca
     1801 ccgagatcgc cgtcgtcctc ggcagccagg accgctacgc cgacgcccgt gcgctcctcg
     1861 gcctgcccgc cgcgccgtaa ttgaacgtac acaccggttc agatgctctc gtacgttcgc
     1921 gcatgctcag gtgaccggcg ctattccgct ggacgtgagt atggtatagt gccggccatg
     1981 ggaagggatt tcaacgaccc gcggggaccg tcgttggaag cgccgtcgcg ctacggttgg
     2041 cgtccggaca cgagcccgga ggagccggcc gagggcgcgt cgctcgctgc cgtcgcgctc
     2101 gcggctaccg ccgtactcga tgcccccgtc gcctcgtcgc tcgatcgcgc gcccgatatc
     2161 ccgctctggc gcccaatagc caggccgctc ccccgccacc gcgcgcgccg gttccctggc
     2221 aagctgcccc tgcacctgct cgtgctggcg acgatcgtag cggtcgtgct cggcggcggc
     2281 tattggagcc gcgccgccat accggcgccc gccgcgcggc ccgccggcac gagcgccctg
     2341 cgcctcggcg agttgccgac caccgtcgga ccgaccagcg acagcggtcc aagcgtgctg
     2401 gccgcggcca cctccggtgg tcgtccggac gtgatgaccg gctacctgtc acagttctcc
     2461 agcgcggtca tccccaatca actgcgcgtg cgcaccatcg tcgccgaggg cagcgagtcg
     2521 atcggcgacc tggccacgca gaccgggctg cggatcgaca cgctcctctg ggccaacggc
     2581 ctgaccgacc cgacgcagga gctgccggcc gggacgcagg tgcgcgtgcc gccggtcgac
     2641 ggcatgctgc atatcgtcca cgacggcgac acgttggaga gcatcgccgc gcgctaccag
     2701 gtcgatgtca cggccatcac cggctatggc ccgaacaacg tgcagacgac ggcggatctg
     2761 gtgccatacc ggctgctgat ggtgcccggt ggcaccatgc ccacgcgcga caaggtcgag
     2821 acctacgtgg tacgcgacgg cgacacgctg gccaccatcg cccagttctt cggggtgcac
     2881 cccaatacca tcctctgggc caacagcctg cctgatggcg atctgatctt ccccggccag
     2941 cacctggcga tcctgccggc cgacggcgtg atggtgacgg tgaaggcggg cgacacggtc
     3001 gagagcctgg ccgcgaccta cagcgtcgag cccaaggcga tccgcgacta ccccggcaac
     3061 ggcctcggcg cggccggtac gctgcgcgtc gatcagttgg tgatgatccc cggtggcaaa
     3121 cccccgaagc cgcccgagcc gcccccggcg ccggccccag ccgcgccggc cgcgcccagc
     3181 gtcgcccaga gccagcccgc tgccgctccg gcgcagcatc ccgccagcgg ccgcttcatc
     3241 tggccgacca gcggcacgat cacccaatac tacggaccga cgagcctgtg gctcgagccg
     3301 gcctacctcg gctacgccca cttccaccag gggctggata tcgccaacgg catgtacacg
     3361 ccgatcgtgg cggcggccag tggcacggtc gccttcgccg gctggaccaa cgtcggctac
     3421 ggctacgcgg tccagatcga ccacggcaat gggctggtca cctggtacgg gcacatggcg
     3481 cagatgccgg cggtgcacag cggacagtgg gtggagcagg ggcaatatct cgggccgatg
     3541 ggcagcaccg gcgcgtcgac cggcgcccac ctccatttcg gcgtgttgaa gaacggcgtc
     3601 tgggacaacc cgctgaacta cctgccgtag tcgcctgcct cttccgttcg ttcgcctgga
     3661 ggagtagacc tccgtgtctg ccctgtaccc cctatgcctc tgcaacgggg acaagggcgg
     3721 cttgggcggc gttgtcgggg gtaggccatg aggcagcgag ggcagatacg gaggtctgcc
     3781 cctccaggcg aagatccggt gggtcggcgt cagtgctggt gctgaggccg ggtgcgctcc
     3841 aggtaggcca tcgcctcacg gtaggtggtg ctgtcgatca gccccgattc cgagtaatag
     3901 gtcagcatcg tgcgcagccg caggatcggc atcacccggt agccgtgctg gtgcagccgc
     3961 tcgacacccc cctgctcgcg gtcgatcagc acgatcgcgt cgcgcacggt cacgccgttg
     4021 tgctccaacg tgcgcgcggt gctgaggatg ctgcgcccgc cggtcatcag atcgtcgatg
     4081 atcagcaccg tctggccggg gatcactttc ccctcgatct gcgcctgctc gccccccggc
     4141 cgcacgtaga tcagcggcgt gtcgctggtc agcgcgtagg cggtcgccag gtgcaacccg
     4201 cccatcggca cgcccgccac cgccgcgaag ccggccagcc gcggccggcg ccgtgcctga
     4261 tccgcctgga tctcctgcgc gatcaggcgc gcgatgctgc gcagcaccgc cggctcgctg
     4321 atcagcacgc gtgggttgat gtagatcggc gagcgcccgg ccgtcgggcc gagatcgaac
     4381 tcgccgaact cgacgcctcc aagatcaaac aatgcctgcg ccagattgag gtttgtctcg
     4441 ccctcgaaca gcacgttcgc gacttcctcc gttgtgattc cccgatcccc gttggtttcg
     4501 ctccggactg caccgctgaa gataccataa aatgttgcga gaagcgtcag gaggtgcgcg
     4561 gtggactatc gtgaggccat cagctacgtc atcgagcgct ccggctacga caagggcttc
     4621 gtggccaatc ccttcgacgc cgagatggtc gggctgcggc gcaccgagtg gctgctggcg
     4681 gcgctcggcc accccgagcg gcgctacccc gcgctgcacg tcgccggcac caagggcaag
     4741 ggttccaccg ccgccaccct ggccgccatc cagcgggcgg ccgggcggcg cgtcggcctc
     4801 tacacctcgc cccacttgca caccttccgc gagcgcatcc agctcgacgg cgagccgatc
     4861 gccgaggatg acttcgccac gctggtcggc gagatcgcgc ccctcaacgc ccggctggcc
     4921 caggagcggc ccgactgggg cgaggcgacc gccttcgagg tcgcgaccgt gctggcgctg
     4981 ctggccttcg cccgctggga ggtggacgtg gcggtagtcg aggtcgggct cggcggacgg
     5041 ctggacgcca ccaacgtgct cctgccggag gtcgcggtga tcaccgccat cagcctcgat
     5101 cacacacgta tcctgggcga tacgctggcc cagatcgcgg ccgagaaggg tggcatcatc
     5161 aagccggggc ggccggtcgt ctcctcgccg cagcgcccgg aggctgccgc caccctggcg
     5221 cgcatcgccg ccgagcgcgg cagcccgatc tccttcgggg gccgcgactg gcaggtggcc
     5281 agcgactccc gatcgctcgc gctcaccggc ccctggggcc gctatgaagg tctctcgctc
     5341 agcctgcgtg gcaactacca gctcgagaac gcgggcacgg cggtggctgc ctgctgggcg
     5401 ctcggccagc gcggcctgcc cacgcctgag gcagccatcc gcgcgggcct ggccagcgtc
     5461 gcctggccgg gccgcttcga gatcatccac cggcagccga tcgtcgtcgc cgacggcgcg
     5521 cacaacgtcg actccgccga acgcctggcc gaggcgctgc gggcggagtt cgcgagcccc
     5581 tcgctcaccc tgatcctggc gatcgcgacc gacaaggatg tcgaggggat ggtgcaggtg
     5641 ctggcgccgc tggcccacac catcatcgcc accgcctcgc acaacccgcg cgccgccagc
     5701 cccgagcgca tcgccgcggc ggcgcgggcg gccggcctgc ccccgccgcg catcgtcgag
     5761 acgccggacg tggccggtgc cctgcggctg gcgctggcag acgccgcgcc gggcgacgcg
     5821 at
//
LOCUS       DASUBI010000696         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_413780, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000696 DASUBI010000000
VERSION     DASUBI010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413780"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2881)
                     /locus_tag="VFU72_13515"
     CDS             complement(<1..2881)
                     /locus_tag="VFU72_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424558.1"
                     /translation="MPDGPYLFVSYASADRERVLPIVARLEAAGVKTWVDRDGIHGGA
                     NYALEIAEAIEHAAALLLFCSPASLASRNVKQELALAWRFERPYLPLLLDLVEIPKDV
                     AYWLEGSQWIELLERPEHDWLADLAQALAPLGLSLTLPVQPHVAPNRARPLLVGRERE
                     QAVLREHLGQMLAGQGSVVLVGGEAGIGKTALAEDVCVQAEEAGALVLWGHAYDLSVT
                     PPYGPWLEIFRRYAGLADDLPPAPAFVGNAEELARIGSQETLFAAVARFFQTVAAQHP
                     LLLVLDDLHWADQSSLDFFRFLARDVAEARLLLLLATYRSDELHRRHPLAMLLPLLVR
                     EANAERIDVRPLDEAGQHALIQSRYGLGAADQSRLEEYLQARAEGNPFFAGELLRTLE
                     EEHLLTRQEERWTLGNLEQVRVPTLLRQVIEGRLARLDEESQRLLEAAAVIGQAIPLD
                     VWTAVGEAAEDTVLEVAERAEGVQVLEAAAGGGGMRFRHALIRETLYEEIPSLRRRRL
                     HRRAGETLAAAASADPDMVAYHFQQAGDARATEWLIKAGERAQRAYAWHVAVDRFEAA
                     FARLTEQDSAARDQAVLLFRVAFLLRFQNARKAVGQLDEARRLALEAGEDALASCCLH
                     LSGLVHCFLGEPRRGIPAMEQAVRDFRALPSHEQGRLQELLGADLGPPEGTLALWLGA
                     VGQLEAGVALARSMLAATPQPALRVGQGDSLYADGLFGLGWSEALLGHPHQAREALQQ
                     ARAIYHTIDHHYLVAYCCWTELEMAQLPYFEGEFDSRSQLAAEGEAAMIKATGAAPER
                     IPPSLPSLCLQILQGNWHEAREVAAAAHGHGRWMIMALASHLLAMLAQRQGDGALAWR
                     IIGECLPDGAPTDPGNTFLSTALPLQRLAVLLVLDAGDLPTARSWLEAHDRWLKWSGA
                     VRGRAQGALGWARYYHAGGDTAQARSMAEQALAHATDPRQPLALSA"
     gene            complement(2881..>3821)
                     /locus_tag="VFU72_13520"
     CDS             complement(2881..>3821)
                     /locus_tag="VFU72_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424559.1"
                     /translation="CLAAFESRHDYFRLAAQTSYAQILFLIPYRAEHVDERRRLAEIA
                     EAAWARVAGVLGTAPPLEVTQFPALLLDGFWERARVGATAARGVHVIWRHIADSVLVT
                     LMAYRGEGDAADPLIQELLPEGPATEPGNCLFLSGSHLQRLAAKLALDAHDLPMARAW
                     LEAHDRWLEWSGAVLGQADGQLGWAAYHHAGGDRDASRRHAEQALALATDPRQPLALI
                     AVHRFLGQLDSEERRYADAEAHLQQSLALADACAAPFERALTLLELAGLRAVQDKPDE
                     ARALLQEIRAICEPLGAKPTLERVAALEQQLVGGGR"
BASE COUNT          620 a         1361 c         1291 g          549 t
ORIGIN      
        1 ccgcgctgag cgccagcggc tggcggggat cggtggcgtg ggccagggct tgctcggcca
       61 tcgaacgagc ctgcgccgtg tcgccgccgg cgtggtaata ccgcgcccaa ccgagcgcgc
      121 cttgcgcccg gccgcggacg gcgccggacc acttcagcca gcggtcatgc gcctccagcc
      181 aggaacgagc ggtcggcagg tcgcccgcgt cgagcaccag gagcacggcg aggcgctgca
      241 acggcaaggc tgtcgagagg aatgtattgc cgggatcggt aggagcgccg tccggcaagc
      301 actcgccgat gatccgccag gcaagtgccc cgtcgccttg ccgctgagcc agcatggcga
      361 gcaagtggct ggcgagcgcc atgatcatcc agcgtccgtg cccgtgcgcg gccgccgcca
      421 cctcccgcgc ctcatgccag tttccctgaa ggatctggag gcacagcgac gggagcgagg
      481 gaggtatgcg ctcaggcgcc gcaccggtcg ctttgatcat cgctgcctca ccctcggcgg
      541 cgagctggct cctgctgtcg aactccccct cgaagtacgg gagctgcgcc atctccagtt
      601 cagtccagca gcagtacgcg acgagatagt ggtgatcgat tgtgtgatag attgctcgtg
      661 cctgctggag cgcttcccgg gcctgatgcg gatggccgag caatgcttcg gaccagccga
      721 ggccgaagag gccatcggcg tataacgaat ccccttgccc cacccgcagc gcgggttgtg
      781 gtgtcgccgc cagcatcgac cgcgccagag cgaccccggc ttcgagctgg ccgaccgctc
      841 cgagccagag cgcgagggta ccctctggcg gtccgaggtc agcgcccaac aactcctgca
      901 gtcgcccctg ctcgtggctg gggagcgcgc gaaagtcgcg taccgcctgc tccatcgccg
      961 ggattcctcg acgcggctcg ccgaggaagc agtgcaccaa tcctgacaga tgcaagcagc
     1021 agctggcgag ggcgtcctcc ccggcctcga gcgcgagccg gcgcgcctcg tccaactgac
     1081 ccactgcctt gcgcgcattc tgaaatcgca gcagaaacgc gacacggaag agcagcacgg
     1141 cctgatcgcg ggccgcgctg tcctgctccg tcagtctggc gaaggcggct tcgaagcgat
     1201 cgaccgcgac gtgccaggcg taggctcgtt gcgcccgctc gccggccttg atcagccact
     1261 cggtggcgcg tgcgtcgccg gcctgctgaa aatggtaggc gaccatatcg gggtcggcgg
     1321 aggccgccgc ggccagggtc tctccggccc gccggtgcag ccgtcgccgg cgcagcgacg
     1381 gaatctcctc gtagagcgtc tcgcggatca gcgcgtggcg aaaacgcatg ccgcctccgc
     1441 ccgcggctgc ttccaggact tgtaccccct ccgcgcgctc cgcaacctcg agcacggtgt
     1501 cttcggcggc ctcgcctacc gccgtccaga catccagcgg aatcgcttgc ccgatgaccg
     1561 ccgcggcctc caagaggcgc tgactctcct cgtccagccg ggccaaacgc ccctcgatca
     1621 cctgccgcag cagcgtcggg acccgcactt gctcgagatt gccgagtgtc cagcgctcct
     1681 cctggcgcgt caggaggtgc tcctcttcca atgtgcgcag cagctcgccc gcgaagaagg
     1741 ggttgccctc ggcccgcgcc tgcagatact cctccaagcg agactggtcg gccgcaccga
     1801 gcccatagcg gctctggatc aaggcgtgct gccccgcctc gtccagcggc cgaacgtcga
     1861 tccgctcggc gtttgcttcc cgaaccagca gcggcaacag catggcgagc gggtgacgcc
     1921 ggtgaagctc gtcggagcgg taggtcgcca gcagcagcag caggcgcgcc tcagccacat
     1981 cacgagccag gaagcggaag aagtcgaggg aggactgatc agcccaatgg aggtcatcga
     2041 gtaccagcag gagggggtgc tgggccgcga ccgtctggaa gaaccgcgcc accgcggcga
     2101 agagcgtctc ctgcgagccg atccgggcca gttcctcggc attgccgacg aaggcgggcg
     2161 cgggcgggag atcgtccgcc agtccggcgt agcggcgaaa gatctccagc caggggccgt
     2221 agggtggcgt aaccgacagg tcataggcat gaccccagag gacgagcgcc cctgcctctt
     2281 ctgcctgcac gcacacgtcc tcggccagcg cggtcttgcc gatgccggct tccccgccca
     2341 ccagcaccac gctcccctgc ccggccagca tctggccgag gtgctcccgc agcaccgctt
     2401 gctcgcgctc gcgcccgacc aacaacggcc gcgcccggtt gggagccacg tggggttgta
     2461 ccggcagagt caggctgagc cccagcggcg cgagcgcctg ggccaggtcg gcgagccagt
     2521 cgtgctctgg acgctccagc agctcgatcc actgggagcc ttccagccag taggccacgt
     2581 ccttggggat ctccaccaga tcgagtaaga gcggcaggta gggacgctcg aatcgccagg
     2641 ccagcgccag ctcctgctta acgttgcggg acgccagcga ggccggcgag cagaagagga
     2701 gcaaggcagc agcgtgctcg atcgcctcgg caatctccag cgcatagttg gccccgccgt
     2761 gaatgccgtc gcggtccacc cacgtcttca ccccagccgc ttccagccgc gcgacgatgg
     2821 gcagaacgcg ctcgcggtcg gcgctggcgt agctgacgaa gaggtaggga ccatcgggca
     2881 ttagcggcct cctcccacta actgctgctc cagcgccgcc acccgctcca gcgtcggctt
     2941 cgcccccagc ggctcgcaga tcgcacggat ttcttgcagc agcgcccgcg cctcgtctgg
     3001 cttgtcctgc accgcccgca acccagcgag ctccagcagc gtcagcgccc gctcgaaggg
     3061 cgccgcgcag gcgtccgcca acgcgagcga ctgctgaagg tgcgcctcgg cgtcggcgta
     3121 gcgacgctcc tcggagtcga gctggccgag gaagcggtgg acggcgatca gtgccaatgg
     3181 ctggcgggga tcggtggcca gcgcgagcgc ctgctcggcg tgccggcggg acgcatcacg
     3241 atcgccgccg gcgtggtgat atgctgccca gccgagttgc ccatcggcct ggccgaggac
     3301 cgcgcccgac cactccagcc agcggtcgtg ggcctccagc caggcgcggg ccatggggag
     3361 gtcgtgcgcg tcgagggcca gcttggcggc caggcgctga aggtgtgagc cgctgagaaa
     3421 gaggcagttt cccggctccg tcgccggccc ctccggcaag agttcttgaa tcagcggatc
     3481 agcggcgtca ccttcgcccc ggtaggccat gagcgtcacg agtactgagt ctgcaatatg
     3541 ccgccagatc acatgcacac cgcgcgcggc tgtggcccca acgcgcgccc gctcccagaa
     3601 accgtcgagc agcaaggccg gaaactgcgt gacttcgagc gggggtgccg tgccgagcac
     3661 gccggccacc cgcgcccagg ccgcctcggc gatctcggca agccggcgcc gctcgtcgac
     3721 gtgctcggcg cggtacggga tgagaaagag gatctgcgcg tagctggttt gagcagcgag
     3781 ccggaaataa tcgtgcctcg actcgaacgc cgccaggcag t
//
LOCUS       DASUBI010000697         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_824262, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000697 DASUBI010000000
VERSION     DASUBI010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824262"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..456)
                     /locus_tag="VFU72_13525"
     CDS             complement(100..456)
                     /locus_tag="VFU72_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424560.1"
                     /translation="MRDTVWYQILGGGLAAAICGMAGTMMTSEAKAVVAALVAGIIVG
                     TGARSWRAIVIAPFGYAAGVAASRALGSVGPAWSIAWLPVTVGLALLGAANVTAVRRR
                     PSASQVAGSHSPENGA"
     gene            616..1503
                     /locus_tag="VFU72_13530"
     CDS             616..1503
                     /locus_tag="VFU72_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5424561.1"
                     /translation="MTFPLRIGIGTGNRLPFPELAGMWREIEELGFDSAWVVDHFLAG
                     QDELTPYFEAWTLIAALSQQTSRIRFGIMVSGNTYRNPALVAKQAVTIDHASGGRVEL
                     GIGAGWWEREHEAYGYTFPPVRDRVEMLEEAAQVITSLLTQERTTFHGAYYTFENTPF
                     EPKSLQKPHLPLVIGAFKPRMLRIAARYADNWNTRGEPEEIAPLAETMRKAARDVGRD
                     PEAIRFSVYAWKHPFTSEEHFRDIVARYHQAGFSDLIFPMPPAEDRPMMERIARQVIP
                     ELRAQYAPGAGAGAGASAE"
     gene            1560..2213
                     /locus_tag="VFU72_13535"
     CDS             1560..2213
                     /locus_tag="VFU72_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5424562.1"
                     /translation="MSTREQVMHIEAASFWEQAMPADEYLAQMSTNHDAFQANMDRTE
                     VTAADRVRFGAQPLKLLVLTEEWCNDSVQFLAATLRLAHEVPAVQVRVLRRDEQREFA
                     AQYPRQDGYNAIPIFILMDADLRELGSLVERPGRVTAEMAEETRRFAQDHPELPGIRR
                     NVDHMPEETKALVKGNNARWRVDMLERWVPYLFDDLATIAERARAEQAQEQAAQARS"
     gene            2386..2799
                     /locus_tag="VFU72_13540"
     CDS             2386..2799
                     /locus_tag="VFU72_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424563.1"
                     /translation="MSGANLLTAEGETVILRQSAAQSISGERVRLERSGAKVIDGKTV
                     ELERSGAGRIQAEQVTLNGGSASLVQARELHMTQGNALLVRADRAELHDSRVALLIGK
                     AEGNPQIALTLPGAAALGAALGAVLVLARWIIGRR"
     gene            2802..3077
                     /locus_tag="VFU72_13545"
     CDS             2802..3077
                     /locus_tag="VFU72_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5424564.1"
                     /translation="MARVLAFLLGIILGAAVGAAIAALLAPESGVELRADIGERIQRG
                     KAAKAEAQQATAEALRQKFRAQVNNQEALRRGTAGEGIITVEPKPSA"
     gene            complement(3114..>3791)
                     /locus_tag="VFU72_13550"
     CDS             complement(3114..>3791)
                     /locus_tag="VFU72_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424565.1"
                     /translation="VAWQGWDGDTWRGVEWKGNPFFVGEVEPCINGQVAASGAYFGQD
                     VRGLVARLLGEQLPDGGWNCEAENGSTRSSFNTTICVLEALLEYERATGSSPEVTAAR
                     LRGQEYLLERRLFRRRSTGELIERDRKGGADWTRFAFPTWWHYDVLRGLDYLRAAGAS
                     PDERVAEAIELVRSKRDSDGRWLLETRYPGVMPGEPDEGEREGRPSRWITLRALRVLR
                     WYEQPGS"
BASE COUNT          622 a         1348 c         1324 g          497 t
ORIGIN      
        1 acccggtcat cccgagcgca gtcgagggat ccgtcctgga cggcgccaat gcctctcgat
       61 gggcaatacc cgtcccctac gccatcgctc actgagcgct caggcgccat tttcaggcga
      121 gtgcgagccg gcgacctgcg acgcgctggg ccgccgccgc acagcggtaa cgttcgcggc
      181 gccgaggagc gcgaggccga cggtgacggg cagccacgcg atcgaccaag ccggccccac
      241 gctgcccagg gctctgctgg ccgcgacgcc ggccgcgtag ccgaacggcg cgatgacaat
      301 cgcccgccac gagcgcgcac ccgtgccgac gataatccca gcgacaaggg ccgccacaac
      361 ggctttcgcc tcgcttgtca tcatcgtccc ggccatgccg catatcgccg ccgcgaggcc
      421 gccgccgagg atctgatacc agacggtgtc ccgcatcatg cctcctcgaa ttccgcagtc
      481 acgatgataa tcgatggccc gcgcgttgac agcaggccgc accgggcgca ccatcgcgag
      541 ggcgggcacg caccattggc agcgagcctg cgaggcgttc gggacgacga gacgacagcc
      601 gtggagggcg accgcatgac ctttccgttg cgcatcggga ttggcaccgg caaccggctc
      661 ccctttccgg agctggccgg catgtggcgc gagatcgagg agctcggctt cgacagcgcc
      721 tgggtcgtcg accacttcct ggctggccag gacgagctga ccccctactt cgaggcctgg
      781 acgctgatcg ccgcgctctc gcagcagacc agccgcatcc gcttcggcat catggtcagc
      841 ggcaacacct accgcaaccc ggcgctggtc gccaagcagg ccgtcaccat cgaccacgcc
      901 agcggcgggc gcgtcgagct gggcatcggc gccggctggt gggagcgcga gcacgaggcc
      961 tacggctaca ccttcccgcc ggtgcgcgac cgcgtcgaga tgctggagga ggcggcccag
     1021 gtgatcacca gcctgctgac ccaggagcgc accaccttcc acggcgccta ctacaccttc
     1081 gagaacacgc ccttcgagcc caagtcgctg caaaagccgc atctcccgct cgtaatcggg
     1141 gctttcaagc cgcgcatgtt gcgcatcgcc gcgcgctacg ccgacaactg gaacacgcgc
     1201 ggcgagccgg aggagatcgc gccgctggcc gagacgatgc gcaaggcggc gcgcgacgtg
     1261 ggccgcgacc cggaggccat ccgcttctcg gtctacgcct ggaagcaccc cttcacctcc
     1321 gaggagcact tccgcgacat cgtggcgcgt taccaccagg ccggcttcag cgacctgatc
     1381 ttcccgatgc cgccggccga ggaccgcccc atgatggagc gcatcgcccg ccaggtgatc
     1441 cccgagctgc gcgcccagta cgcgccaggg gccggagcag gggcgggagc aagcgcggaa
     1501 tagccgccgg ggggtaacgt tacaggcggt gtaccgggca ccgcgaggcg gagagcccca
     1561 tgagcacgag ggagcaggtc atgcacatcg aggccgcgtc attttgggaa caggccatgc
     1621 cggccgacga gtacctcgcc cagatgagca ccaaccacga tgcgttccag gcgaatatgg
     1681 atcgcaccga ggtgaccgcg gcggatcggg tgcgcttcgg cgcgcagccg ctgaagctgc
     1741 tggtgctcac cgaagagtgg tgcaacgact cggtgcagtt tctggcggcg acgctgcgcc
     1801 tggcgcatga ggtgccggcg gtacaggtgc gcgtgctgcg gcgcgacgag cagcgcgagt
     1861 tcgcggccca gtatccgcgc caggacggct acaacgccat cccgatcttc atcctgatgg
     1921 acgctgatct gcgtgagctg ggttcgctgg tcgagcgccc ggggcgcgtg accgccgaga
     1981 tggcggagga gacgcgccgc ttcgcacagg atcatccgga gctgccgggc atccggcgca
     2041 atgtcgacca catgccggag gagaccaagg cgctggtgaa gggcaacaac gcacgctggc
     2101 gcgtcgacat gctggagcga tgggtgccct acctcttcga cgatctggcc accatcgccg
     2161 agcgggcccg ggcagagcag gcacaggagc aggcagcgca ggcccgttcc tagccccagc
     2221 gcgccgtcgc gcggccagcc aaccgagcac gcgatcaggg agggaggacg cgtggcagag
     2281 gatcaagcca gacgcggcga aggcacgccg gagtcttccg acgagaccac ttcgtccgct
     2341 gcgggcacgc cagccgctcc gtccgctcca tcaggcacga gcgacgtgag cggcgccaac
     2401 ctgctgacgg ccgagggcga gacggttatc ctgcggcaga gcgccgcgca gtcgatcagc
     2461 ggcgagcggg tgcggctgga gcgcagcggg gcgaaggtca tcgacggcaa gaccgtcgag
     2521 ctggagcgca gcggcgccgg gcgtatccag gccgagcagg tgacgctcaa cggcggctcg
     2581 gccagtctgg tgcaggcgcg cgagctgcac atgacccagg gcaacgcgct gctcgtgcgc
     2641 gccgatcggg ccgagctgca cgactcgcgc gtggccctgc tgatcggcaa ggcggagggg
     2701 aatccccaga tcgcgctgac gctgccggga gccgccgcgc tgggcgccgc gctcggggcg
     2761 gtgctggtgc tggcgcgctg gatcatcggg aggagatagc gatggcgcga gtgctcgcgt
     2821 tcctgctggg catcatcctc ggcgcggccg tcggggcggc catcgcggcg ctgctggcgc
     2881 cggagagcgg tgtggagctg cgggcggaca tcggcgagcg catccagcgc ggtaaggccg
     2941 ccaaggccga ggcccagcag gccaccgccg aggcgctgcg acagaagttc cgcgcccagg
     3001 tcaacaacca ggaagcgctg cggcgcggca cggccggcga ggggatcatc accgtggaac
     3061 cgaagccgtc ggcctgaggc cggttccgtt tcgattcggg cgcgtcgccg tctttacgag
     3121 cccggctgct cgtaccagcg cagcacgcgc agggcgcgga gggtgatcca gcggctcggc
     3181 cggccctcac gctcaccttc gtccggttcg cccggcatca cgccgggata gcgggtctcc
     3241 agcagccagc ggccgtcgct atcgcgcttc gaccgcacca gctcgatggc ctcggccacg
     3301 cgctcgtcgg gcgaggcgcc ggcggcgcgc aggtagtcca acccccgcag cacgtcgtag
     3361 tgccaccagg tcgggaaggc gaagcgcgtc cagtcggcgc cgcccttgcg atcgcgctcg
     3421 atcagctcgc cggtcgaccg ccgtcggaag aggcgacgct cgagcaggta ctcctgcccg
     3481 cgcagccggg ccgcggtcac ctccggactg ctcccggtcg cgcgctcgta ctccagcagg
     3541 gcctccagca cgcagatggt ggtgttgaac gacgagcgcg tcgagccgtt ctcagcctcg
     3601 cagttccagc cgccgtcggg caactgctcg ccgagcagcc gcgcgacgag gccccgcaca
     3661 tcctgaccga agtaggcgcc gctggccgcc acctgcccat tgatgcacgg ctcgacctcg
     3721 cccacgaaga agggattgcc cttccactcc acgcctcgcc aggtgtcgcc gtcccagccc
     3781 tgccaggcca c
//
LOCUS       DASUBI010000698         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_986560, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000698 DASUBI010000000
VERSION     DASUBI010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986560"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="VFU72_13555"
     CDS             complement(<1..2359)
                     /locus_tag="VFU72_13555"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5424566.1"
                     /translation="MAENARQPYLFVSYASSDRDRVLPIIDRLEAAGVPLWVDREGIG
                     GGASYAQEIAEAIAHAAAVLLLCSPASLASRNVKQEIALAWRHERPYLPLLLEPASVP
                     PDLEYWLEGIQWIEVLELGPDVWLPKVLAALERLGFDRAAVKPPPVPSPQGERRIKLP
                     YEPGALLGREVELAALVGLLRQPDVRLVTLCGPGGIGKTRLAIAAGQVLVDDFAQGVV
                     FVDLATVRDPALVGPAIANALDLQLSGEPDPREQLAAALVHQQLLLILDNFEQVLDAA
                     PLLAELLASTASLKILVTSRSGLQLRAEHEYPVAPLRLPDAGRPLPLAELAASPAVRL
                     FVARAQAVEPGFALTPDNAAAVADICRRLEGIPLALELAAARLRVLPPAALLGRLEHR
                     LALLTGGARDQPARHQTLRDTIAWSYDLLAPAEQDLFARLAVFAGGAPLAGIEAVRGE
                     AEHDDGVLDRLDALLRASLLRRGEDRGDEPRFTMLETIREYATEQLASRPDGQRTYRA
                     HAAFYLALAQAAEPALFGASQAAVLEQLEREHGNLRAALAWLRAGGDAGGALRLATTL
                     WRFWYLRSHLTLGIARLGDLLEQNGAADPALRAKALLGLGALSFKAGTLPRAQAYLEE
                     SVRLYRALGDAAGLGWALVFLGHVAGAQHDDAAGYAQVEESLALFRQVGDQAGMARAL
                     NALGETSRSAGDDARAESFYAEALALDRAGGNRAGAAIRLHNLGYVDLHRGAIDQAAA
                     RFEESLLIQQELGDAAGVAQGVEGLAATAVAVGRCRLAAQLFAAAE"
     gene            complement(2599..3681)
                     /locus_tag="VFU72_13560"
     CDS             complement(2599..3681)
                     /locus_tag="VFU72_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5424567.1"
                     /translation="MRTNRRRSRITLHARYAIGLLPVGFMILSLVGTLFLSAPPAALA
                     ASVTTTDDLNLRAGPSLNDRVLTVMPAGSAVAVTGDPQNGFYPLSYHGLSGWAMGVYL
                     SLGAPAPASTGSGATSSAVTTDDLNLRAGPSLGADVLTVMPAGASVTLKGDTQNGFAA
                     VSYGGRDGWAAIAYLKPGTASASAAVATAAAPAPAASGWTTTTDLNLRAGPSTGDTIL
                     TVMPSGSAVTVTGAAQNGFSPVSYQGTSGWAYGAYLAQGGNGGGQSDAAPSGSYTSDQ
                     IVGFIYAAADKYGQSRADMLRVARCESNLNPRAVGGGSFGLFQFTRGTWATTPYAGDD
                     IFDPQANANAAGWMWSVGRRGEWVCQ"
     gene            complement(3913..4590)
                     /gene="tenA"
                     /locus_tag="VFU72_13565"
     CDS             complement(3913..4590)
                     /gene="tenA"
                     /locus_tag="VFU72_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU5424568.1"
                     /translation="MENQTADSITTQLWREIAPIYAVILEHPFLRGLTDGTLPEAAFR
                     YYVVEDARYLRDFARCLALAAAKAPRDDWGVFFAEQAAGALVVERALHEGFFRDWGVD
                     AGEAGAETAPANLAYTSYLLRVAYAEPFAELLGALLPCFWIYWEVGKALAAAGSPNPL
                     YQRWIDTYAADEYAASVRQALATMDACAAELSPERRACVARHFVTTSRYEWLFWDAAW
                     RRETWPV"
BASE COUNT          672 a         1721 c         1743 g          622 t
ORIGIN      
        1 cctcggccgc cgcgaagagc tgcgcggcca gccggcagcg cccgaccgcc acggcggtgg
       61 cggcgagccc ctccacccct tgcgccacgc ccgcggcgtc gccgagctct tgctggatca
      121 gcaggctctc ttcgaaacgc gccgccgcct ggtcgatcgc cccccggtgg aggtcgacgt
      181 agccgaggtt gtgcagccgg atcgccgccc cggcccggtt gccgccggcg cgatcgagcg
      241 ccagcgcctc tgcgtagaac gactcggcgc gtgcgtcatc accggccgag cggctggtct
      301 cgcccagcgc gttcagggcg cgcgccatcc ccgcctgatc gccgacctgg cggaagagcg
      361 ccagcgactc ctcgacctgg gcgtagccgg ccgcgtcgtc gtgctgggcg ccggccacgt
      421 gccccagaaa gaccagcgcc cagcccagcc cggccgcgtc gcccagcgcg cggtagagcc
      481 gcacgctctc ttcgagatac gcctgggcgc gcggcagcgt gccggctttg aacgacagcg
      541 cgccgagccc caggagcgct ttcgcccgca gggccggatc ggccgcgccg ttctgctcca
      601 gcagatcgcc cagcctggcg atcccgaggg tgagatggct gcgcagatac cagaagcgcc
      661 agagggtcgt ggccagccgg agcgcgccgc cggcatcgcc gcccgcgcgc agccaggcca
      721 gcgccgcgcg caggttgccg tgctcgcgct cgagctgctc caggacggcg gcttggctgg
      781 ctccgaacag cgccggctcc gccgcctggg ccagcgccag gtaaaacgcc gcgtgggcgc
      841 gataggtccg ctggccatcc gggcggctcg ccaactgctc ggtggcgtac tcgcggatcg
      901 tctccagcat ggtgaagcgc ggctcgtcgc cgcggtcttc cccccggcgc agcaggctgg
      961 cgcgcagcag cgcgtcgagg cggtccagca cgccgtcgtc gtgttccgcc tcgccgcgga
     1021 ccgcctcgat gccggccagc ggcgcgcccc cggcaaacac cgccagccgc gcgaagaggt
     1081 cctgctcggc gggcgccagg aggtcgtagc tccaggcgat cgtgtcgcgc agcgtctgat
     1141 ggcgcgcggg ctggtcgcgc gcgccgccgg tcagcagcgc cagccggtgc tccagccgcc
     1201 cgagcagcgc cgccggcggc aggacccgca gccgcgccgc cgccagctcc agcgccagcg
     1261 ggatcccctc cagccgccgg cagatgtcgg ccacggccgc ggcgttgtcc ggcgtaagcg
     1321 cgaagcccgg ctccacggcc tgggcgcgtg cgacgaagag ccgcacggcg ggggaggccg
     1381 ccaactcggc gagcggcagc gggcggccgg cgtcgggcag ccgcaggggc gccaccggat
     1441 actcgtgctc ggcgcgcaac tgcagcccgg accggctggt gaccaggatc ttcaacgacg
     1501 ccgtcgacgc cagcaattcg gccaggaggg gcgcggcgtc cagaacctgc tcgaaattgt
     1561 cgaggatcaa cagcagctgc tggtgcacca gcgcggccgc caactgctcg cgcgggtccg
     1621 gctccccgct caactggagg tccagcgcgt tggcgatcgc cggcccgacc agcgccgggt
     1681 cgcgcaccgt cgccagatcg acgaagacga cgccctgggc gaagtcatcc accagcacct
     1741 ggccggcggc gatagccagg cgcgtcttgc cgatcccgcc ggggccgcac agggtcacga
     1801 ggcgcacgtc cggctggcgc agcagcccga cgagcgcggc cagctcgacc tcacggccca
     1861 gcagcgcgcc cggttcgtag ggcagcttga tgcgtcgctc cccctgcggc gacggaaccg
     1921 gcggcggctt gacggcggca cggtcaaacc cgaggcgttc gagggcggcc agcaccttcg
     1981 gcagccacac gtcggggccg agttcgagca cctcgatcca ctggataccc tcaagccagt
     2041 attcgaggtc ggggggcacg ctggcgggtt cgagcagcag cggcaggtat gggcgctcgt
     2101 ggcgccaggc cagcgcgatc tcctgcttga cgttgcgcga ggcgagtgag gccggggagc
     2161 acagcagcag cacggcggcc gcgtgggcga tggcctcggc gatctcctgg gcgtagctgg
     2221 cgccgccgcc gatcccctcg cgatcgaccc acagcggcac acccgccgcc tccagccggt
     2281 cgatgatcgg cagcacccgg tcgcgatcgc tactggcgta gctcacgaag aggtagggtt
     2341 gacgggcgtt ctcggccacg accaatcccc ttcggcccga cactattgcc ataagcgtat
     2401 gcggtgcccg gcgacggcgc aacggggaac gggcgcggag ccgctcgaac gtgtcgtgtt
     2461 ccgaatcagt gggaagggga gccgggcggc ggctgaaccg gccgcccgcc tgaccgcgtg
     2521 accagccacg ggcgcgcgga cgagccgcga gcagtgagcc ccttgggctc actgctcagc
     2581 cggagtcgac agccggcgct attggcagac ccactcgccg cgccggccga ccgaccacat
     2641 ccagccggcg gcgttggcgt tggcctgcgg gtcgaagata tcgtcgccgg catagggcgt
     2701 ggtggcccag gtgcctctgg tgaactggaa caggccgaag gagccgccac cgaccgcgcg
     2761 cgggttcagg ttcgactcgc agcgcgccac gcgcagcatg tcggcgcgcg actgcccgta
     2821 cttgtccgcc gccgcgtaga tgaagccgac gatctggtcg ctggtgtagc tgccgcttgg
     2881 cgccgcgtcg ctctggccgc cgccgttccc gccctgggcc aggtaggcgc cgtaggccca
     2941 gccactggtg ccctggtagc tgaccgggga aaagccgttc tgggccgcgc ccgtcaccgt
     3001 gaccgcgctg ccgctcggca tcaccgtcag gatcgtgtcg ccggtgctgg ggccggcacg
     3061 caggttgaga tcggtagtgg tggtccagcc gctcgccgcc ggggccgggg ccgcggctgt
     3121 ggccacggcc gcgctggcgc tggccgtgcc gggcttgagg taggcgatcg cggcccagcc
     3181 gtcccgcccg ccgtaggaga ccgcggcgaa gccgttctgg gtgtcgccct tcagggtcac
     3241 gctcgcgccc gccggcatga ccgtcagcac gtcggcgccc aggctcggcc cggcgcgcag
     3301 gttgaggtca tcggtggtca cggcgctgga cgttgccccg gagccggtgc tcgcgggggc
     3361 gggcgcgccg agcgagaggt agacgcccat cgcccagccg ctgaggccgt ggtagctgag
     3421 cgggtagaag ccattctggg ggtcaccagt gaccgcgacg gcgctgccgg cgggcatgac
     3481 cgtcagcacg cggtcgttga ggctggggcc ggcgcggaga ttgaggtcgt cggtcgtggt
     3541 gaccgaggca gcgagcgccg ccggcggcgc ggagaggaac agcgtgccga cgagggagag
     3601 aatcatgaat cccacgggca gcaggccgat ggcgtagcgt gcgtgcagcg tgatgcggct
     3661 gcgtcgtcga ttcgtccgca agtgcgatcc cttcgcttcc ccaactccga cccggacggc
     3721 tccagcggga ggcgcgtccc gagttggagg gagttatgac attgtgtagc caattcgtca
     3781 acacaccgcc gcacgaacga ccgtatccgg cgtaccgggg cggcgctaag gcgcgccggg
     3841 tcaggtcgag ccgcgggccg gcgcgccgcc catgctcctc gacaggcagc cgtccaggcg
     3901 cgcggagagc gcctagaccg gccacgtctc ccggcgccag gcggcgtccc agaagagcca
     3961 ctcgtagcgg ctggtggtca cgaagtggcg cgcgacgcag gcgcggcgct ccggcgacag
     4021 ctcggccgcg caggcatcca tggttgccag cgcctggcgc acgctggcgg cgtactcgtc
     4081 ggcggcgtag gtgtcgatcc agcgctggta gagcggattg ggcgagccgg ccgccgccag
     4141 cgccttgccg acctcccagt agatccagaa gcaggggagc agcgccccca gcagctcggc
     4201 gaagggctcg gcgtaggcca cccgcagcag gtacgaggtg taggccagat tggcgggcgc
     4261 cgtctctgcc ccagcctcgc ccgcgtccac gccccaatcg cggaagaacc cctcgtgcag
     4321 cgcccgctcg accaccagcg ccccggcggc ctgctcggcg aagaagacgc cccagtcgtc
     4381 gcgcggcgcc ttggccgccg ccagcgccag gcagcgcgcg aagtcgcgca ggtagcgcgc
     4441 gtcctcgacc acgtagtagc ggaaggccgc ctccggcagc gtgccgtcgg tcagcccccg
     4501 gaggaaggga tgctccagga tcaccgcgta gatcggcgcg atctcccgcc acagttgcgt
     4561 ggtgatcgag tcggcggtct ggttttccac gccgcacctc ttctgtgatg gacggaaccg
     4621 ccgggacagt cgggacagta tagtgcccac gcaacgattc gtattgtcgg tgtccggcgg
     4681 gcgtcttcct gtaggggcag acccctgtgt catcgactcc aactaattcg tgtcctgcgt
     4741 ggtctttgga gagcgggg
//
LOCUS       DASUBI010000699         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1214059, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000699 DASUBI010000000
VERSION     DASUBI010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214059"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1165
                     /locus_tag="VFU72_13570"
     CDS             101..1165
                     /locus_tag="VFU72_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEU5424569.1"
                     /translation="MTTRIDTAAIRRHYPLAEVVERYGVALHHCGAHLVGQCPFHADH
                     EPSLVLYTDHALDEHFHCFGCGAHGDVIRFVELIERLPFRDAVVVLAGLPASGTAHCP
                     DTGREPARRPPGVRNPAHRACLAVATALYHQQLLRDRAALAYCAQRGLDHATIVRCQL
                     GYATGANLVGALFRAGLDLAPAVRAGLLTATGRERLTGRLIVPEVRASWPHWLIGRAL
                     DARTRPRYLGLPGCKPLLGWAEVQGRAAVCLTEGVIDRLVLSGWGLPALALAGTHTTP
                     AALAALARFPRLYLVLDNDAAGRAATATLQAALGARARPVALPTGCKDVAELAERPGG
                     RAVFVRALARASREPTALAA"
     gene            1195..1599
                     /locus_tag="VFU72_13575"
     CDS             1195..1599
                     /locus_tag="VFU72_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424570.1"
                     /translation="MSTTPHPSTRSAINTMMRAFVAELAAAGVPDPLNQQITLAALWD
                     DLCRLSHERSPAAVECRLAGATAASTARASTTGPVSAAFNRLLGELTARHLPDPLAQR
                     FTVGLVWAVLCQLGGEDPPALVQALLHAPVLC"
     gene            1657..2061
                     /locus_tag="VFU72_13580"
     CDS             1657..2061
                     /locus_tag="VFU72_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5424571.1"
                     /translation="MSQSYNAIHLVGRLGRDPELRHTATGQPVVRFSLATDRPTRAGA
                     PPETDWHEIICWEAQAEFAERYLTKGRLVLVAGALHYRTWTGKDGQQRRGAEIRATRL
                     VPLDSRPETTLASGRADDPDQALDPDGDDVPF"
     gene            2366..2650
                     /locus_tag="VFU72_13585"
     CDS             2366..2650
                     /locus_tag="VFU72_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424572.1"
                     /translation="MVSIAACLPADQQLLLATRAQPPARLADLFATFVGQFSAAERAR
                     FDALARQVSADYARTDPDQAARFRSRLNRRLSDRYHTLVVWTVDRDGPPR"
     gene            2634..3212
                     /locus_tag="VFU72_13590"
     CDS             2634..3212
                     /locus_tag="VFU72_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEU5424573.1"
                     /translation="MAHRADARQVSLLAPDEATLVHLLRENSRLLHQLVARHAVDRHA
                     APAEAPIVSCPEDLAAYLGPEMAELAQEQLRVVLLDSRNRVLGVQLVFQGGLNATVIR
                     LADCFREALRANAAAIVLVHNHPSGDPTPSADDCCLTGLAGRFGDALGIDVLDHLIIG
                     GDRHTSLRRLGLYTPPRDERAAPSPQAGVPPP"
     gene            3226..>4553
                     /locus_tag="VFU72_13595"
     CDS             3226..>4553
                     /locus_tag="VFU72_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424574.1"
                     /translation="MMETTSPSTLFTPVTPLADATTTIDQEIGDAAVTQRAGIAAARA
                     GLDPAQIERLPFDLSALEEEGVFVNVDARGFGLLDRRLDWHALGVTLPRGAGVAFHPP
                     RCGLLPDRYRLPLIRPAARAHAALHHYSYRFQLTETLFETPSYRWVPWRAFAAFEQEF
                     GAAQAALQHAQAALLADYPAVRAEVVALFRQLAAESARRLAATGHAAPDGFTGRVVAG
                     VLDHFPSAADIAERLTLRYRVGVILLGSEMIAEQRRARAARQALESAEAAARLVQQRQ
                     AARERLVQQELWAAEERLRRQLAVEEEERRQEVTVKERLRQVKLDAARERLKDELSPL
                     EEGCRQLQAQVYDAVSAIRDTLQQRGTLQGAAVKRARRLARWVKLMNWQDDRQLAALI
                     AELERLASQPARQRQHDAAPLADVLDDLIALTCASARAVAEPHRLDALEV"
BASE COUNT          645 a         1752 c         1468 g          688 t
ORIGIN      
        1 accgcgcagc actgacgccg gatggtgcaa ccgtagtgcg cgtgtgccgg ccctcccgtt
       61 tcctcggcct gctacccgca gctgcgctgg cccaggcggc atgaccaccc ggatcgatac
      121 cgccgcgatc cggcgccact acccactggc cgaggtcgtc gagcgttacg gcgtcgcgct
      181 gcaccactgc ggcgcgcacc tcgtcggcca gtgcccgttc cacgctgatc atgagccgag
      241 cctggtgctc tacaccgatc acgcgctgga tgagcacttc cactgcttcg gttgcggcgc
      301 gcacggcgac gtcatccgct tcgtggaact catcgaacgc ctgccgttcc gcgacgcggt
      361 cgtcgtgctc gccggcctcc cagcgagcgg caccgcgcac tgcccagaca ccggccgcga
      421 gccagcgcgc cgccccccag gtgtccgtaa ccccgcgcac cgcgcctgcc tcgcggtcgc
      481 caccgcgctc taccaccagc agttgctgcg cgatcgtgcg gcgctggcgt actgcgcgca
      541 acgcggtctc gaccacgcca cgatcgtccg ctgccagctg ggctatgcca cgggcgcaaa
      601 cctcgtcggc gcgctcttcc gggccggact ggacctggcg ccggccgtgc gcgccgggct
      661 gctgaccgcc accggccgtg agcgactgac gggccggctc atcgtgccgg aggtgcgtgc
      721 cagctggccg cactggctga tcgggcgggc gctcgacgcc cggacgaggc cacgctacct
      781 ggggttgccc ggctgcaagc cgctcctcgg ttgggcggag gtgcagggtc gcgccgcggt
      841 ttgcctcacc gaaggcgtca ttgaccggct ggtgctcagc ggctggggtc tacccgccct
      901 cgcgctggcc ggcacccata cgacccccgc cgcgctcgcc gcgctggcgc gcttccctcg
      961 cctctacctg gtcctggaca acgacgccgc cggccgggcg gcgaccgcca cgctccaggc
     1021 ggcgcttggc gcgcgggcgc gcccggtcgc tctccccacc ggctgcaagg acgtcgcgga
     1081 actggcggag cgccccggcg gccgtgccgt attcgtgcgg gcgctcgctc gggccagcag
     1141 ggagcccacc gcgctggcag cgtaacccga tcccctacta tctaggagga accaatgagc
     1201 accacaccac atccatccac ccgctcggcc atcaatacga tgatgcgcgc gttcgtcgcc
     1261 gagctagcgg cggcgggcgt gcccgacccg ctcaatcaac agatcacgct cgccgcgtta
     1321 tgggatgacc tttgccgcct gagccatgaa cgatcaccag ccgccgtgga gtgccggctg
     1381 gcgggcgcca ccgccgcatc tactgccaga gcgtcgacta ccggcccggt gtcggcggcc
     1441 ttcaatcgcc tgctcgggga attgacagca cggcacctac ccgatccgct cgcgcagcgt
     1501 tttactgttg gcctcgtctg ggcggtcctc tgccagctcg gcggcgaaga cccacccgcg
     1561 ctggtgcagg cgctgctgca tgcgccggta ctctgctgat tgcccgccgc cctgactggt
     1621 ctggcgatta ccgagctgca acgaaggagg ctcaacatgt cccagtcata caacgcgatc
     1681 catctggttg gccgtctcgg ccgcgatccg gaactgcgcc acaccgcgac cgggcagccg
     1741 gtcgtccgct tcagcctcgc caccgaccgg cccacccgcg ccggtgcccc gcccgagacc
     1801 gactggcacg agatcatctg ctgggaagcg caggcggaat ttgccgagcg ctacctgacg
     1861 aaaggacgcc tcgtcctcgt agccggcgcg ctgcactatc gcacctggac cggcaaggac
     1921 ggccagcagc ggcgcggggc cgagatccgc gcgactcgtt tggtcccgct cgacagccgg
     1981 ccagaaacga ccctggccag cgggcgggca gacgatcccg accaggcgct cgatcctgat
     2041 ggtgatgacg tcccgttctg accaccaccc gcgcctccgc tgccggcggt gccgccgagg
     2101 ctgtctcctc atcctcaccg tgccgcaaca gatcactcca ccggctgcgt cacggcaact
     2161 ggtgcttgtg gtcagaccgg cacgaccata cctccgctac ttcatcgtct acgcctttcc
     2221 tggcctgccc gatcaacccc tgccctatcc acccgcagcc tgaccggcac cccggcagca
     2281 agtctgccgc tccctcctgt ccaggagcgt accaactggg agcgacgtcc gagacgccgc
     2341 cccgccgcat agtgcggagg tcgtgatggt ctcaatcgct gcgtgtctgc ctgctgatca
     2401 gcaactgttg ctcgccaccc gtgcccagcc gcccgcccgc ctcgcggatc tcttcgccac
     2461 tttcgtcggg cagttttccg ccgccgagcg cgcccggttc gacgccctgg cccggcaggt
     2521 cagcgccgac tacgcgcgca ctgatccgga ccaggccgcc cgctttcggt cccggctgaa
     2581 tcgccgcctg tccgaccgct accacacgct ggtcgtctgg acggtcgacc gcgatggccc
     2641 accgcgctga cgcccggcag gtgagcctgc tagcccccga cgaagcgacc ctcgtacatc
     2701 tcttgcggga aaactcccgg ctgctacacc agctggtcgc tcgccacgcg gtggatcggc
     2761 acgccgcgcc ggcggaagcg ccgattgtca gctgcccgga ggacctcgct gcatacctcg
     2821 gccccgagat ggccgagctc gctcaggagc aattgcgtgt cgtcctgctc gattcacgga
     2881 accgcgtgct cggggtgcag ctggtcttcc agggtggcct caacgccacc gtgatccgct
     2941 tggccgactg ctttcgcgag gcgctccgcg cgaatgcggc agccattgtc ctcgtccaca
     3001 accatccctc gggcgatccc acgccatcgg cggacgattg ctgcctcacc gggctggccg
     3061 ggcggttcgg cgacgccctc ggaatcgacg tgctggacca cctgatcatc ggcggcgacc
     3121 gccacaccag cctgcgccgc cttggtctct acaccccgcc gcgcgacgag cgcgccgcgc
     3181 cgtcgccgca ggctggcgtc ccgccgccat agccaggagg acagcatgat ggagacaact
     3241 agcccgtcca cgctcttcac accagtcacg cccctagcgg acgctaccac cactattgac
     3301 caggagatcg gcgacgctgc ggtgacgcag cgcgccggca tcgccgctgc ccgcgcgggt
     3361 ctagacccag cgcagatcga gcgcctgccc ttcgacctct cggcgctaga agaggagggc
     3421 gtgttcgtga acgtggacgc gcgcggtttc ggcctgctgg accgccgtct cgactggcac
     3481 gcgctcggcg tgacgttgcc gcgcggcgcc ggggtcgcgt tccacccgcc gcgctgcggt
     3541 ctgctgcctg atcgttaccg gctgcccctc atacgcccgg ccgcccgcgc ccacgcggcg
     3601 ctgcatcact attcctaccg cttccagctg accgagaccc tcttcgagac gccgagttac
     3661 cgctgggtgc cctggcgcgc gttcgccgcc ttcgagcagg agttcggcgc ggcgcaggcg
     3721 gcacttcagc acgcccaggc cgcgctcctc gccgactacc cggccgtgcg cgccgaggtc
     3781 gtcgccctct tccgccagct cgccgctgag tcggcacgcc ggctggccgc gaccggccac
     3841 gccgcccctg atggcttcac cgggcgggtt gtggcgggcg tgctggacca cttccccagt
     3901 gcggccgata tcgccgagcg gctgaccctg cgctaccgcg tcggcgtgat cctgctgggg
     3961 agtgaaatga tcgccgagca gcgccgggcg cgggcggcgc ggcaggcgct ggagtcagcc
     4021 gaggctgccg cccggctggt ccagcagcgg caggctgccc gtgagcgcct ggtgcaacag
     4081 gaactctggg cggcggagga gcgccttcgc cggcagctgg cggtcgagga agaagaacgt
     4141 cgccaggagg tcaccgtgaa agagcggcta cgccaggtga aactggacgc cgcgcgcgaa
     4201 cgcctcaagg acgaactgag tccgctggag gagggctgcc gtcagctcca ggcccaggtc
     4261 tacgacgccg tcagcgcgat ccgcgacacc ctgcaacagc gcggcacgct ccagggcgcc
     4321 gcagtcaaac gggcgcggcg gctggcccgc tgggtcaagt tgatgaactg gcaggacgac
     4381 cggcagctcg ccgcgctcat cgccgaactc gaacgcctgg ccagtcagcc ggccaggcag
     4441 cgtcagcacg acgccgcccc gctcgccgat gtgctcgatg acctcatcgc cctgacctgc
     4501 gccagcgctc gcgcggtggc cgagccacac cgcctcgatg cgctggaagt ttg
//
LOCUS       DASUBI010000700         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_292359, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000700 DASUBI010000000
VERSION     DASUBI010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292359"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..292
                     /locus_tag="VFU72_13600"
     CDS             47..292
                     /locus_tag="VFU72_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424575.1"
                     /translation="MGFGQRPDTRDLESWGLRPSRRPGANSAVVEDAVSGALIMLADD
                     LPDEAYASLFEIEFETAPEGACFFYDVIENRWRHNGE"
     gene            532..1002
                     /locus_tag="VFU72_13605"
     CDS             532..1002
                     /locus_tag="VFU72_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424576.1"
                     /translation="MATTHRRSPLHFDPRRMRLERGRRHWPLSPVARSREAARQPDGA
                     LVAIVAPQHWRRERVVRQLEAAGFRTLVGHDLALMATRLRAAQPDVVVLGVALQRDPA
                     NRRLVERLRHDPATSQIPILDAPAIHDSSAALCDLTLVTMVRALLATRDAARAG"
     gene            1133..1357
                     /locus_tag="VFU72_13610"
     CDS             1133..1357
                     /locus_tag="VFU72_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424577.1"
                     /translation="MNELTVVAESAVHPGRKLLQARDGAGYLQLDHHATPLPLSSADF
                     KRLRAMSHYRTQPRSRRLSFAPHEEYDLAD"
     gene            complement(1428..1811)
                     /locus_tag="VFU72_13615"
     CDS             complement(1428..1811)
                     /locus_tag="VFU72_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU5424578.1"
                     /translation="MTATTPIISLTPEAMKELRDYMDEQGTPEAMLRVFVSPGGCSGL
                     SYGMSVEEDADEEDFVVEQDGVRILVDQFSAMYVEGAEIDYINSLMGGGFTVRNPNAV
                     SSCACGHSFDTGENGGTAQGCGCGH"
     gene            complement(1878..2681)
                     /locus_tag="VFU72_13620"
     CDS             complement(1878..2681)
                     /locus_tag="VFU72_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEU5424579.1"
                     /translation="MAERVTPRLGVKWSPVAREVLPLVDLIEVNGWSLAAGPPPLPTL
                     LHNLDLDFSLTAPGILGEAWAARVAEAVSRTGSPWFSLHLGFSAERVRFESHMLPVSA
                     LLDRATCLERTTAAARFLRTRLQVPLLLENLDYCPEGAYEHVCQPDFIADAVAAVDCG
                     LLLDLAHLRVSADWLGYQPRDYLQRLPLERVVEVHLSGPRRLDARLDDSHRELTGDDE
                     ELLMWLLSHCAPRAVVLEYERDAGRLRAQLLRLREIIATAGTRVAPTAD"
     gene            complement(2674..3795)
                     /gene="leuB"
                     /locus_tag="VFU72_13625"
     CDS             complement(2674..3795)
                     /gene="leuB"
                     /locus_tag="VFU72_13625"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5424580.1"
                     /translation="MSGQTFEILLVPGDGVGPEVTAEARAVLEAVGSKVGATFRFREA
                     RIGGCAIDAYGTALRDEEIAAARASDAILFGAAGGPKWDNLPKEVRPERGLLRLRQEL
                     GLFANLRPVKVFDALADSTPLKPETVRGVDLLVLRELTGGLYYGQPSERRRDERGRSA
                     VDTLPYHESEVERIVDLAFRLAAQRHGKVTSVDKANVLNTSQLWREVAVEVAARYPAI
                     GFEHALVDSCAMRLVSRPRDFDVLVMENLFGDILSDEAAVLAGSLGMLPSASLNGRPP
                     ERAGSAAALFGLYEPVHGSAPDITGQGIANPLGAILSAAMLLRFSLGLDTAAEAVEQA
                     VGATLAEGLRTADIAAGGPAVSTRQMGERVRGHAVERLG"
     gene            complement(3792..4319)
                     /locus_tag="VFU72_13630"
     CDS             complement(3792..4319)
                     /locus_tag="VFU72_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5424581.1"
                     /translation="MQARGKVWKFGDDVDTDVIIPARYLVTIDPDELAEHVMEDLDPE
                     FASKVQPGEIIVAGKNFGCGSSREHAPIAIKAAGVKVVVAESFARIFYRNAINIGLAV
                     VESPEAVRATETGDILEVDTDEGGVLNIRTGDSYPAKPYPEYVQQIIDAGGLMQAVAQ
                     RIKARQADEVKAPAE"
BASE COUNT          688 a         1594 c         1447 g          692 t
ORIGIN      
        1 atcccgcgtg ctcccgggcc gatgcccgcg cagagaggag cccacgatgg gattcggaca
       61 gcggcctgat acgcgggatc tggagtcgtg ggggctccgg ccgtcgcggc gaccgggggc
      121 gaattcggcg gtcgtcgagg acgcggtctc cggcgcactg attatgctgg ccgacgatct
      181 cccagatgaa gcctatgcca gcctcttcga gatcgagttc gaaacggcgc ccgagggcgc
      241 ctgcttcttc tacgatgtca tcgaaaaccg ctggcgacac aacggggagt gagggtgccc
      301 gccgcctgaa cgacccccgc tcgcgatctc gtggttgaca ggaggggtcc gaaccagcta
      361 agctaggagc gagttatatc gatccacgat gttgtgtgcg acagacccca gccccagtag
      421 cgattcctcc gcgtcgccgt cctcacatcc acggtttcgc gtcgagcaat caacggattc
      481 ccaccgcgcg cgtcgcaccc tcgccgtgct ccacgacgag aggagcctga gatggctacc
      541 acccaccgtc gcagcccact gcacttcgat ccccgccgca tgcgcctaga gcgtggccgg
      601 cgccactggc cgctctcacc ggtggcgcgc tcgcgtgagg ccgcccgcca gccggacggc
      661 gcgctcgtcg ccattgtcgc gccgcagcac tggcgccgcg agcgggtcgt gcgacagttg
      721 gaggccgccg gttttcgcac gctggtcggg cacgatctgg cgctgatggc cacgcgcttg
      781 cgcgccgccc agccagatgt cgtcgtgctc ggcgtcgcgc tgcagcgcga tccggccaat
      841 cgccgcctgg tcgagcggct gcgacacgac ccggccacca gccagatccc gatcctcgac
      901 gcgcccgcga tccacgacag ctcggcggcc ctgtgcgacc tgacgctggt cacgatggta
      961 cgggcgctgc ttgccacccg ggacgccgcc cgggccggct aagccgcaag ccggcgctcg
     1021 cacctgccga tcctcggcag tcatgactgt tagcctgcgc tagtggcacg gaacatgcga
     1081 ataaatatct ccgtgacaca tcgcatcgtt ctggtgatgg gcggggattg agatgaacga
     1141 attgacagtc gtggccgaga gcgcggtcca ccccggtagg aagctcctac aggcgcgcga
     1201 cggcgcgggc tacctgcagc ttgaccacca cgcgacgcca cttcctttgt cgagcgcgga
     1261 cttcaaacga ctgcgcgcca tgagccacta tcgcacccag ccgcgcagcc gcaggctgtc
     1321 gttcgccccc cacgaagagt acgacctggc cgactaggcc ggcacaagcg acacgaaacg
     1381 ggcgctcgca cgagcgcccg ttctccttgt cctacaagtt gcagccgcta gtggccgcag
     1441 ccgcagccct gcgccgtgcc accgttctcg ccggtgtcga acgagtgacc gcaggcgcaa
     1501 ctggagaccg cgttggggtt gcgcacggtg aaaccgccgc ccatcagact attgatgtag
     1561 tcgatctcgg cgccctcaac gtacatggcg ctgaattggt ccacgaggat gcgcacgccg
     1621 tcctgctcga cgacgaagtc ctcctcatcg gcgtcctctt ctaccgacat gccgtaggag
     1681 agccccgaac agccgccggg tgacacgaag acgcgcagca tggcctccgg cgttccctgc
     1741 tcatccatgt aatcgcgcaa ttccttcatc gcctcgggtg tcagggaaat gattggtgtg
     1801 gtcgccgtca tcctcgtagt cctccagtct ttcgcaatcg ccgagttggt taaatgatac
     1861 ccccactttg ccgcctgtca atcggccgtc ggcgcaactc gcgtgccggc ggtcgcgatg
     1921 atctcgcgca gccgcaggag ctgcgcacgc agccgcccgg cgtcccgttc gtactctaag
     1981 accacggcac gcggcgcgca atggctcagt agccacatca gcagctcctc atcgtccccg
     2041 gtcagctcgc gatggctgtc gtcgagccgc gcatcgagcc ggcgcggccc gctgagatgc
     2101 acctcgacca cgcgctccag gggcagtcgc tggaggtagt cacgcggctg gtagccgagc
     2161 cagtcggcgc tgacccgcag gtgcgccagg tcgagcagca gcccgcagtc gaccgccgcg
     2221 acggcatcgg cgatgaagtc cggctggcag acgtgctcgt aggcaccctc cggacagtag
     2281 tcgaggttct ccaacagcaa cggaacctgg agacgcgtgc gcaggaagcg tgcggccgcg
     2341 gtcgtgcgct ccaggcaggt agcgcggtcg agcagggccg agaccggcag catgtggctc
     2401 tcgaagcgca cgcgctccgc gctgaagccg aggtgcaggc taaaccaggg gctgccggtg
     2461 cgtgacaccg cctccgccac gcgcgccgcc caggcctcgc ccaggatgcc cggcgcggtc
     2521 aggctgaagt cgagatcgag gttgtgcagc agggtcggca gcggcggcgg ccccgccgcc
     2581 agcgaccagc cgttgacctc gatcaggtcg accagcggca gtacctcgcg cgccaccggc
     2641 gaccacttca cccctagccg gggggtgacg cgctcagcca aggcgttcga ccgcgtgccc
     2701 gcgtacgcgc tcgcccatct gccgtgtgct gaccgccggc ccgccagccg cgatatccgc
     2761 cgtgcgcagc ccctcggcca gcgtcgcgcc gaccgcctgc tctacggcct ccgccgccgt
     2821 gtcgagaccc agcgagaagc gcagcagcat ggccgcgctc aggatggcgc ccagcgggtt
     2881 ggcgatcccc tgcccggtga tgtcgggcgc ggacccgtgc accggctcgt agaggccgaa
     2941 gagggccgcg gcgctcccgg ctcgctcggg cggacggccg ttcaggctgg ccgaggggag
     3001 catccccagc gagccggcca gcaccgccgc ctcgtcgctc aggatgtcgc cgaagaggtt
     3061 ctccatcacc agcacgtcga agtcgcgcgg gcggctgacc agacgcatgg cgcaggagtc
     3121 gaccagggcg tgctcgaagc cgatcgcggg gtagcgcgcg gcgacctcga ccgccacctc
     3181 gcgccagagc tgcgaggtat tcagcacgtt ggccttgtcc acgctggtga ccttgccgtg
     3241 ccgctgggcc gccaggcgaa aggcgagatc gacgatgcgc tcgacctcgc tctcgtggta
     3301 cggcagggtg tcgacggcgc tcctgccgcg ctcgtcgcgc cgccgctccg agggctggcc
     3361 gtagtagagc ccgccggtca gctcgcgcag caccagcagg tccacgccgc gcaccgtctc
     3421 cggcttgagc ggcgtgctgt cggccagcgc gtcgaagacc ttgaccgggc gcaggttggc
     3481 gaacaggccg agttcctggc gcagccgcag cagcccgcgc tcggggcgga cctccttggg
     3541 caggttatcc catttcggcc caccggccgc cccgaagagg atggcgtcgg acgcgcgcgc
     3601 ggcggcaatc tcctcgtcgc gcagcgcggt gccgtaggcg tcgatggcgc aaccgccgat
     3661 ccgcgcctca cgaaaccgga aggtcgcgcc gaccttggag ccgaccgcct ccagcacggc
     3721 gcgcgcctcg gcggtcacct ccggcccgac accgtcgccg ggcaccagaa gaatctcgaa
     3781 ggtctgcccg ctcattcggc tggtgccttc acctcgtccg cctggcgcgc cttgatccgc
     3841 tgggccaccg cctgcatcag cccaccggcg tcgatgatct gctgtacgta ttcggggtag
     3901 ggcttggccg ggtagctgtc acccgtgcgg atgttcagca cgccgccttc gtcggtgtcg
     3961 acctccaaga tgtcgccggt ctcggtggcc cgcaccgcct ccggcgactc gaccaccgcc
     4021 agcccgatgt tgatcgcatt gcggtagaag atgcgcgcga acgactcggc caccaccacc
     4081 ttgaccccgg ccgccttgat cgcgatcggc gcgtgctcgc gcgaggagcc gcaaccgaag
     4141 ttcttgccgg ccacgatgat ctcgcccggc tgcaccttgc tggcgaactc gggatcgagg
     4201 tcttccatca cgtgctccgc cagctcgtcg gggtcgatgg tcacgaggta gcgggcgggg
     4261 atgatcacgt cggtgtcgac gtcgtccccg aatttccaga ccttgccgcg tgcttgcatg
     4321 cgtttgctcc ttacttgccc tcacccccaa cccctctccc aatactggga gaggggagca
     4381 cgtcggggga gcacagccgt gttcccgtag ttactccccc t
//
LOCUS       DASUBI010000701         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1054999, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000701 DASUBI010000000
VERSION     DASUBI010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054999"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFU72_13635"
     CDS             complement(<1..330)
                     /locus_tag="VFU72_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5424582.1"
                     /translation="MRVLIVDDEAEIRDVLSLLLAGEDIEVLAAEDGWEGLRLARDEQ
                     PDLILMDIHMPVLDGVTATALLKRDPRTAHIPVIAMSAGRNLRRQSSGLSADGLLAKP
                     FDLDELLA"
     gene            698..1075
                     /locus_tag="VFU72_13640"
     CDS             698..1075
                     /locus_tag="VFU72_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424583.1"
                     /translation="MEQLLAELEMLRRQLDAALENATQGARESHESWTQRLETAESIG
                     AELSARGKEEIQEERRREMLCLGQAAGLAWVAMRLARIVDQAQQATGGRRPDPTETAE
                     PPAASADQAFTERSQLWDVGEIG"
     gene            complement(1083..2075)
                     /gene="gmd"
                     /locus_tag="VFU72_13645"
     CDS             complement(1083..2075)
                     /gene="gmd"
                     /locus_tag="VFU72_13645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5424584.1"
                     /translation="MKTALITGITGQDGSYLAEFLLEKGYRVVGMQRRTSTETVGRVA
                     HLRDKIELVQGDLLDQLSLIDLLRRYQPDEIYNLAAQSFVPTSWQQPVLTGEFTALGV
                     TRVLEAIRLIKPDARFYQASSSEMFGKVREVPQHETTPFYPRSPYGVSKVYGHWITVN
                     YRESYDLYAVSGILFNHESPRRGLEFVTRKVTHGAARVKLGLDRELRLGNLDAQRDWG
                     YAPDYVRAMWLMLQQQRPDDFLIATGRTHTVERLCELAFGALGLDWRDYVEVDPRFMR
                     PAEVDLLVGDASKARAVLGWKPETSFEAMIELMVDADLRMLKGEDVPLGMIGTR"
     gene            2197..3009
                     /gene="dapF"
                     /locus_tag="VFU72_13650"
     CDS             2197..3009
                     /gene="dapF"
                     /locus_tag="VFU72_13650"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5424585.1"
                     /translation="MPGIPFRKLSGAGNDFILIDERECALDPAWLAQRICRRARSLGA
                     DGLILVGPDPAGVRVRFYNPDGSRAEMCGNGSRCAARDALELGLAAGPSFTLLSDSGA
                     LAVEARSDGSYAVAMPLPSDLQPGLLTVAVRGEPVEVHALWVGVPHAVLFVPDVDALG
                     DDEVTRLGRALRHDPAFPAGTNVNLVTPLPDGSLRQRTYERGVEALTLACGTGATASA
                     TTAHQLLELPWPLTVQVDGGRLAIDHHDGHTWLRGPARLIARGVLEPDALRD"
     gene            3024..3431
                     /locus_tag="VFU72_13655"
     CDS             3024..3431
                     /locus_tag="VFU72_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU5424586.1"
                     /translation="MAQRGHKLFTLAEALALLPRLRELLTALQTERREMAEELRALEQ
                     LTSAMRANGHAAEAARREKRLGELTAAIRSGLQQMATLGVEVKDLDSGLVDFPSLREG
                     RVVYLCWRLDEPTIAYWHELDTGFLGREPLDQG"
BASE COUNT          589 a         1277 c         1259 g          512 t
ORIGIN      
        1 cgccagcagc tcgtcgaggt cgaagggctt ggccagcagg ccgtcggccg acaacccgct
       61 ggactggcgc cgcaggttgc gcccggccga catggcgatg accgggatgt gcgccgtgcg
      121 cgggtcgcgc ttgagcagcg cggtcgcggt cacgccgtcg agcaccggca tgtggatatc
      181 catcaggatc aggtcgggct gctcgtcgcg cgccagccgt aggccctccc agccatcctc
      241 cgccgcgagc acctcgatat cttcgccggc cagcagcagc gacaggacat cgcggatctc
      301 agcctcatcg tcgacgatca ggacacgcat gatggcggct ccttggctcg tgctttcctg
      361 ggctcccgtc cgcgccgccc ctgggcatcc gcccggcgcg aggcaccgca aacccaggac
      421 gggaatgagc gggagaagtc gtccgccatc gctttgcccc atcgccatgc aggagcgtga
      481 atcgaacgcg caccaaagcg agacggaaca cggcaatgat ccaccctggc ctgggtgaac
      541 gtgtagccca aaagtcctga gcaccactgg ccagaattcc tcatcccaaa cccggaccga
      601 ggtccgccgc gcgcctctgc cgcggccccc accatgagag aggagtgggg gcgggcgtga
      661 gtcagcgacg ggagccgccg ggagggacca aaggcgcatg gagcaactgc ttgccgaact
      721 ggagatgctg cgccggcagc tcgacgccgc gctggagaac gccacgcagg gcgcgcgcga
      781 gagccacgag agctggaccc agcggctcga gacggccgag tccatcggag cggagctctc
      841 ggcgcgcggc aaggaggaga tccaggaaga gcgccggcgc gagatgcttt gtctcgggca
      901 ggcggccggg ctggcctggg tcgcgatgcg gctggcgcgc atcgtggacc aggcccagca
      961 ggcgaccggc ggcagacggc ccgaccccac ggagaccgcc gaaccgccgg cggcgagcgc
     1021 cgatcaggcc ttcaccgaac ggagccagct ctgggacgtg ggcgagatcg gctgatcgcg
     1081 gcctagcgcg tcccgatcat ccccagcggt acgtcctcgc ccttgagcat gcgcagatcg
     1141 gcgtcgacca tcagctcgat catcgcctcg aacgacgtct ccggcttcca gccgagcacc
     1201 gcccgcgcct tgctggcgtc ccccaccagc aggtcgacct cggccgggcg catgaaacgc
     1261 ggatcaacct cgacatagtc gcgccagtcg agccccagcg cgccgaaggc cagctcgcag
     1321 aggcgctcga cggtgtgggt gcgcccggtc gcgatcagga agtcgtcggg ccgctgctgc
     1381 tgcagcatca gccacatggc gcgcacgtag tcgggcgcgt agccccagtc gcgctgggcg
     1441 tcgagattgc ccagccgcag ctcgcggtcg agccccagct tgacacgcgc cgccccatgc
     1501 gtcactttgc gcgtcacgaa ctccaagccg cggcgtggag attcatggtt gaacaatatt
     1561 ccacttactg cgtataagtc atagctctcg cggtagttga ccgtgatcca gtggccgtag
     1621 accttgctga cgccgtaggg ggagcggggg tagaacgggg tcgtctcgtg ctgcggcacc
     1681 tcgcgcacct tgccgaacat ctcggaggag ctggcctggt agaagcgtgc atcgggcttg
     1741 atcagccgga tcgcctccag cacgcgggtc acgccgagcg cggtgaactc gccggtcagg
     1801 accggctgct gccaggaggt cggcacgaac gactgcgccg ccaggttgta gatctcgtcg
     1861 ggctggtagc ggcgcaggag gtcgatcagc gagagctggt cgagcagatc cccctgcacc
     1921 agctcgatct tgtcgcgcag gtgcgccacc cgcccgaccg tctcggtgct ggtgcgccgc
     1981 tgcataccga cgactcgata gcccttctcc agcaagaact ccgccagata ggagccgtcc
     2041 tgcccggtga tccccgtgat cagcgccgtc ttcatcaacc gctccatcca ccgtcccaat
     2101 cgtggccgcg agcggcaagt gtaccggatg gcgccgcccc aggggcctgc cgtgctacac
     2161 tcgcgcggat acgatcgctg acctgggagc acgaagatgc cggggatacc gtttcgcaaa
     2221 ttgagcggcg cggggaacga tttcattctg atcgacgagc gggagtgcgc gctcgacccc
     2281 gcctggctgg cccagcgcat ctgccgccgg gcgcgctcac tcggcgccga cggcctgatc
     2341 ctggtcgggc ccgacccggc cggcgtgcgc gtgcgcttct acaacccgga cggctcgcgc
     2401 gccgagatgt gcggcaacgg cagccgctgc gcggcgcgcg acgcgctcga actcgggctc
     2461 gccgccggcc caagcttcac cttgctcagc gatagcggcg cgctggcggt cgaggcccgg
     2521 tccgacggca gttacgccgt ggccatgccg ctgcccagtg atcttcagcc cggactgctg
     2581 accgtcgcgg tacgcggtga gccggtcgag gtccacgcgc tgtgggtcgg cgtgccccac
     2641 gcggtcctct tcgtccccga cgtcgacgcg ctgggcgacg acgaggtgac ccgcttgggc
     2701 cgcgcgctgc ggcacgatcc ggccttcccc gcgggcacca acgtcaacct ggtcacgccg
     2761 ctgccggacg gcagcctgcg ccagcgcacc tacgagcgcg gcgtcgaggc gctgacactc
     2821 gcctgcggca ccggcgccac cgccagcgcc accacggctc accagctcct cgagctcccc
     2881 tggccgctga cggtccaggt cgacggcggc cggctggcca tcgaccacca cgacggccac
     2941 acctggctcc gcggtccggc gcggctgatc gcgcgcgggg tgctcgagcc ggacgcgctc
     3001 cgcgactagg agaggtggct gcgatggccc agcgcgggca caagctgttc acgctggcgg
     3061 aggcgctggc gctgctgccc cggctgcgcg agctgctgac ggcgctacag acggagcgcc
     3121 gggagatggc cgaggagctg cgcgcgctgg agcaactgac gtcggcgatg cgcgccaacg
     3181 gccacgccgc cgaggccgct cgccgcgaga agcggctggg cgagctgacc gccgcgatcc
     3241 ggagcgggct gcagcagatg gcgacgctcg gggtggaggt caaggatctc gacagcggcc
     3301 tggtcgactt ccccagcctg cgcgagggcc gcgtcgtcta cctctgctgg cgcctcgacg
     3361 agccgaccat cgcctactgg cacgagctcg acaccggctt ccttggccgc gagccgctgg
     3421 atcaaggcta aggtggtggc aaacgacagg gactgaacgc gtgtgagggc ggatccttcg
     3481 acaagctcag agcctgccct gagcgcaagc gaaggatccg tcctgcattg cgatcaagcg
     3541 tattcgaagg gcagcgaccc gctggttacg gcacgcgatc atgacgtatg gaacgaggag
     3601 aacgcatggc tgagttgacc cgcgaggcga tcccggc
//
LOCUS       DASUBI010000702         8452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1100969, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000702 DASUBI010000000
VERSION     DASUBI010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100969"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFU72_13660"
     CDS             complement(<1..415)
                     /locus_tag="VFU72_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424587.1"
                     /translation="MRAVRVRGWLIAGLLLALLAPPLAACGASTAPGSSATATPPVAT
                     AAPVTTPSPAPAPSPTPRPSPAPSPTPSPSPAASPAPVAGALEVPGPGDRVTLPLHIL
                     AHLPQPAEQVTATLRWKDGTSRRFDMPVQRASDGTG"
     gene            complement(446..1591)
                     /locus_tag="VFU72_13665"
     CDS             complement(446..1591)
                     /locus_tag="VFU72_13665"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5424588.1"
                     /translation="MAGFDTRVSTGVMLRRPAVCILLALALALLLPATALAAPANDPA
                     PRLVYFPQTGHYLAYGFLDYWLEHGQVAIFGYPISEELTDPATGLTVQYFERAVFEYH
                     PDNPPAWRVELRRLGALATANRQSEPPFQPLNAQSDDNCTYFPQTGHRLCFGFRAYWQ
                     QNGGLAIFGYPLSEEFQENGYTVQYFERARFEYHPSNPPAWRIELGLLGSNAAAGAHV
                     ATSPLAQSPSVETYDPGLWYVPAPPEKPTAAATPPAGAPSNQAKWIEVDLSDQYLRAW
                     TYSTPVYYTRVSTGVPGHQTPTGTFAVYAKYAEDDMTGGTPGVDYYNLPDVPDVMYFY
                     QAYAIHGAYWHNNFGSVMSHGCVNLPLDAADWMFNWTPVGTTVWIHS"
     gene            complement(1663..2544)
                     /locus_tag="VFU72_13670"
     CDS             complement(1663..2544)
                     /locus_tag="VFU72_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424589.1"
                     /translation="MSAPEQFEQLEPGMERIVVAGRGDFADQVARAVAALAELTLVGQ
                     IDPGAALPQGAGLVLCLAEPAQRVSVIQQASEAGLPVATLPLAETGDAARAALGAGRV
                     AQVSALRGYAALETLRAESAGGALGRRYGVFATHRVRAGRRDLFASLGVPLLHYVYEL
                     AGAPVSVAQTTRTALHGDEADAWFVIAQTSGGPLLTVEFGAILPPESPAPEELLVEAT
                     GSEGVLRGEPLRQSVLVSNRQGTSARPWWSALGPGFLGAALGAVQAPDPAREQGFLDF
                     LGAVLRAAEAGQPASVA"
     gene            complement(2541..3617)
                     /locus_tag="VFU72_13675"
     CDS             complement(2541..3617)
                     /locus_tag="VFU72_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5424590.1"
                     /translation="MPRLKVGLIGAGTIANSAHLPAIAHLHDQLELVAVADVRPEAAE
                     QAARRYGAESWYSDYRQLLARPDIDMVDICTPEFLHGEQVVAAAEAGKHVLCEKPMAN
                     SLADADAMIAAAKRNQVKFMVGHSRRFTARYRAIQQAISEGAIGEVRLVRENERRAAS
                     MYNVMRRWAGYWRPVDERPWILLAEFTYGAALTNAVHETDLARWFAGSEAASVYSASQ
                     ITAPEGEVPDFISYTITFQNGAIGAAEVVNNLPPRYPYFHMMEVFGSEGMIRATDPPM
                     SPLEHWTGDERGMRFPENFNVLLHVDDAYVREIEQFAQAVREDTPVPLDPWNARQALA
                     IALAAVESSKTGQVVSLPREGGAA"
     gene            4095..4994
                     /locus_tag="VFU72_13680"
     CDS             4095..4994
                     /locus_tag="VFU72_13680"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5424591.1"
                     /translation="MPEFDPNARLDTSQVEDRRGMGGGGGVGRGVAVGGAGGGLVILA
                     LILSMIFGVPIDVSGPSSQPVSQNPAAAPASGTLAQNCQTGADAAAQQDCRIVAYVDS
                     IQAYWGSEFSRRGMQYTKSKTVLFTGSTSTGCGQATTEVGPFYCPVDKTVYLDLGFFD
                     QLRSQFGAQGGPFAEAYVLAHEYGHHVQDLVGTLAKYPNPTQGAQGTSVRIELQADCF
                     AGVWADNATRTGFITQLSQADIADGLNAAAAVGDDRIQQEFQGHVNPESWTHGSSAER
                     QHWFSVGYQSGDMGSCNTFSGSL"
     gene            5252..6223
                     /locus_tag="VFU72_13685"
     CDS             5252..6223
                     /locus_tag="VFU72_13685"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU5424592.1"
                     /translation="MTTPCPDPHHVQRVRQNLLDWYRANRRDLPWRRTRDRYHILVSE
                     VMLQQTQVDRVIPKYHEFLARFPTLQALAEAPTAEVIRAWSGLGYNRRAVNLQRAAQA
                     VVERHGGVMPRERAALEALPGVGRYTAGAIACFAYEQDAGFVDTNIRRVLHRVFHGPE
                     LPHEQATPRELEALAAALVPHGEGYDWNQGLMELGALICTARRPLCLTCPLHDDCRAA
                     PAIQTVLAGLPKGTRRKQEGPFEGSTRFYRGRVIHALRALSAGESLDLASLGPQVRAD
                     FGPEHLPWLRELVGGLTRDGLAEVAGVAEERAAYDASAPDELRVRLP"
     gene            complement(6233..7003)
                     /locus_tag="VFU72_13690"
     CDS             complement(6233..7003)
                     /locus_tag="VFU72_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424593.1"
                     /translation="MRIGVNLGPTADRAALLAAAQSADTLGYDALGLLDHYHAPNPEW
                     RYLCGWSLYGALAVMTSRIRLVPLVIDRLNYLPGILAREAAALAVLSDGRFELGIGAG
                     DFFAEARAWGMTVPDAPARISGLRETVLALRRLWSGEAVRVEGRELRLLDAASATLPP
                     EPPRIIVGAGRSHRLIRSAVAYADEINVYADDDLIGYARDQIDAAGRSVSLSTYAWDW
                     PDDIAGALARWERQGVARAFLTFWHPFDTLAAATTLMS"
     gene            complement(7086..>8452)
                     /locus_tag="VFU72_13695"
     CDS             complement(7086..>8452)
                     /locus_tag="VFU72_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424594.1"
                     /translation="TGGARDLPARQRTMRDAISWSYDLLDAEERRLFARLALFAGGGT
                     LEAIEAICAQDAHEADAVLERAMALLNNNLLARDMADDHEDGAGERGPRLRMLEPIRQ
                     YAAERLAESDDREALRRRHAEYFLSFTEGARSEQAIWLRRIEREHDNLRAALGWAFER
                     ADDTARLTTGLRLASALWWFWEVRGYLAEGRAWLERGLSAGADVPAELRAAACYGLGA
                     LAIRQRDYAAADAFYGESLALSRALGDGTGVARALTTLGEVALRRGELARARGLLTES
                     LALKHGLGDAPGVADALNIAGLVARDAGDDGEARALFEESLALMRELEQRWGVLIVLG
                     NLGHLTLRQGDDARAAALLYESLGLAQELGAKWSIAYCLEGLAAAAAGRGAIERAARL
                     WGAAEALRAAIDAPVPPNLAPHYEAQVAAARLRADERAWEAAWAAGRALPLEDAVSEA
                     LEAV"
BASE COUNT         1213 a         3098 c         2960 g         1181 t
ORIGIN      
        1 atccggtgcc gtcactggcg cgctgcaccg gcatgtcgaa gcggcggctg gtgccgtcct
       61 tccagcgcaa ggtcgcggtc acctgctcgg ccggctgcgg cagatgcgcc aggatgtgca
      121 agggcaaggt gacccggtca cctggccccg gcacttcgag cgcgcccgcg accggagcag
      181 gcgaagccgc cgggctgggg ctcggcgtcg gcgagggcgc cgggcttggg cgcggcgtcg
      241 gggaaggcgc cggcgcgggg gacggagtgg tcaccggcgc tgccgtcgct accgggggcg
      301 tggcggtcgc cgaagacccg ggcgccgtcg acgcaccgca cgccgccagc ggcggcgcca
      361 gcagcgccaa cagaaggccc gcgatcagcc aaccacgcac ccgaaccgca cgcatgatcc
      421 tccgtccctc ccgcacccgt cgccgtcagg agtgaatcca gacggtcgtg ccgaccggcg
      481 tccagttgaa catccagtcg gccgcgtcga gcggcaggtt gacgcagccg tggctcatga
      541 cggagccgaa gttgttgtgc caatacgcgc cgtggatcgc gtaggcctgg tagaagtaca
      601 tgacatcggg cacatcgggg aggttgtaat agtcgacgcc cggggtgccg ccggtcatgt
      661 cgtcctcggc atacttggcg tagacggcaa aggtgccggt cggcgtctgg tgcccgggca
      721 ccccggtaga aacccgcgtg tagtagaccg gcgtggagta cgtccaggct cgcaggtact
      781 ggtccgagag atcgacttcg atccacttcg cctgattgct cggtgcgccg gccggcggcg
      841 tggccgctgc ggtcggcttc tccggcggcg ccggcacgta ccagagaccc gggtcatagg
      901 tttccacgct cggtgattgc gccaagggtg aggtcgcgac atgcgcgccc gcggccgcgt
      961 tcgatccgag cagacccaac tcgatccgcc aggcgggcgg gttgctgggg tgatactcga
     1021 agcgcgcccg ctcgaagtac tgcaccgtat acccgttctc ctggaactcc tccgagagcg
     1081 ggtagccgaa gatggccagc ccgccgttct gctgccagta ggcgcggaag ccgaagcaga
     1141 gccggtggcc ggtctgggga aagtaggtgc agttgtcgtc gctctgcgcg ttcagcggct
     1201 ggaagggcgg ctcactctgg cgattggccg tcgccagcgc gcccaggcgg cgcagctcga
     1261 cgcgccacgc gggcgggttg tcggggtggt actcgaagac ggcgcgctcg aagtactgca
     1321 ccgtcaggcc ggtggccggg tcggtcagct cctcggagat cggatagccg aagatcgcga
     1381 cctgcccgtg ctccaaccag tagtcgagga agccgtaggc gaggtagtgc ccggtctggg
     1441 ggaaatagac cagccggggc gcggggtcgt tggcgggcgc cgccagcgcg gtagccggaa
     1501 gcagcagcgc gagcgccagc gccagcagga tgcagacggc tggccgccgc agcatgaccc
     1561 ccgtgctcac tctggtgtcg aagccagcca tcacggtctc ccttcgcact ccttctctat
     1621 gaagaggcga accgccacga acgttacggc gccggtgagc gcctaggcga cgctggcggg
     1681 ctggccggct tcggcggcgc gcaggacggc gccgaggaag tcgaggaagc cctgctcgcg
     1741 cgccgggtcg ggcgcctgca cggcgccgag cgccgcgccc aggaagccgg ggccgagcgc
     1801 gctccaccag gggcgcgcgc tggtgccctg gcggttgctg accagcaccg actggcgcag
     1861 cggctcgccg cgcagcacgc cttcggagcc ggtcgcctcg accaagagct cctccggcgc
     1921 cggcgactcc ggcggcagga tggcgccgaa ctcgaccgtc agcagcggcc cgccgctggt
     1981 ctgcgcgatc acgaaccagg cgtccgcctc gtcgccgtgg agcgccgtgc gcgtggtctg
     2041 cgccacgctg accggcgcgc cggccagctc gtagacgtag tgcagcagcg gcacgcccag
     2101 cgacgcgaag aggtcgcgcc ggcccgcgcg cacgcggtgc gtggcgaaga cgccgtagcg
     2161 gcggccgagc gcaccgcccg ccgactccgc ccgtagcgtc tccagcgcgg cgtagccgcg
     2221 cagcgcactc acctgcgcca cccggccggc gcccagcgcg gcccgcgccg cgtcgccggt
     2281 ctccgccagc ggcagcgtcg ccaccggcag cccggcctcg ctcgcctgct ggatcacgct
     2341 cacccgctgc gccggctcgg ccaggcagag caccagcccg gcgccctgcg gcagcgccgc
     2401 gccggggtcg atctggccga ccagcgtcag ctcggccagc gccgcgaccg cccgcgcgac
     2461 ctggtccgcg aagtcgcccc ggccggcgac cacgatgcgc tccatccccg gttcaagctg
     2521 ctcaaactgc tccggcgcgc tcacgcggcg cctccttccc gcggcaggct gacgacctga
     2581 ccggtcttgc tcgactcgac cgcggccagc gcgatcgcca gcgcctggcg cgcgttccag
     2641 gggtcgagcg gcaccggcgt gtcctcgcgc accgcctggg cgaactgctc gatctcgcgc
     2701 acatacgcgt cgtcgacgtg gagcaagacg ttgaagttct cggggaagcg catccccctc
     2761 tcgtcgccgg tccagtgctc cagcggcgac atcggcggat cggtggcgcg gatcatcccc
     2821 tcgctgccga agacctccat catgtggaag taggggtagc gcggcggcag gttgttgacc
     2881 acctcggccg cgccgatggc gccgttctgg aaggtgatgg tgtagctgat gaagtcgggc
     2941 acctcgccct cgggcgcggt gatctgggag gcggagtaga ccgaggcggc ctcggagccg
     3001 gcgaaccagc gcgccagatc ggtctcgtgc accgcgttgg tcagcgcggc gccgtaggtg
     3061 aactcggcca gcaggatcca ggggcgctcg tcgaccggac gccagtaacc ggcccagcgc
     3121 cgcatgacgt tgtacatcga ggcggcgcgg cgctcgttct cgcgcaccag ccgcacctcg
     3181 ccgatggcgc cctcgctgat cgcctgctgg atggcccggt agcgcgcggt gaagcgccgg
     3241 ctgtgcccga ccatgaactt gacctggttg cgtttggcgg cggcgatcat ggcgtcggcg
     3301 tcggccagcg agttggccat cggcttctcg cagaggacgt gcttgccggc ctcggcggcg
     3361 gccaccacct gctcgccgtg caggaattcg ggcgtgcaga tgtcgaccat gtcgatgtcg
     3421 ggccgcgcca gcagttggcg gtagtcgctg taccaggatt ccgcgccgta gcggcgggcg
     3481 gcctgctcgg cggcctcggg gcgcacgtcg gccaccgcga ccagctccag ctggtcgtgc
     3541 aggtgcgcga tggccggcag gtgggcgctg ttggcgatcg tgccggcgcc gatcaagccg
     3601 actttcaagc gtggcatggg agcctcctct ccatcgggcg catggtaacc cgccggggcc
     3661 gggattggga gtggctggac ggaccgctgg ggagccctca ccccggggag ccctcacccc
     3721 aaccctctcc ccggggagcc ctcaccccaa ccctctcccg tgcgcgggag agggagagtg
     3781 cccatcgaga aggcttgacg gtggggggac tgtccctata cctaaccctt gtctttagcc
     3841 acctcccctc tcccgcgcac gggagagggg ccgggggtga gggaactcgc gttggggtga
     3901 gggagctccc gccgcggatg agggcgccga acggcggcac gctggatgca tctgccgtgc
     3961 ctcatggagc cggcgtccgc gccaattgct ttcgtcgtgt ccgcgcgggc ctggcgcgcc
     4021 ggctccgctt ttcccggcca acgcgcgata attccggccg ggagcgaccg acgatctcgc
     4081 gagagggaac gacgatgccg gaattcgacc ccaacgcgcg cctcgatacc tcgcaggtcg
     4141 aggaccgccg cggcatgggc ggcggtggcg gtgtgggacg cggagtggcg gtcggcgggg
     4201 ccggcggcgg cctggtgatt ctggcactga tcctctcgat gatcttcggc gtgccgatcg
     4261 acgtcagcgg cccctcttcc cagccggtca gtcagaaccc cgccgccgcg ccggcctccg
     4321 gcacgctggc gcagaactgc cagaccgggg ccgacgccgc cgcccagcag gactgccgca
     4381 tcgtggccta cgtcgacagc attcaggcgt attggggcag cgagttcagc cgccgcggca
     4441 tgcaatacac caagtccaag acggttctct tcaccggctc gacctccacc ggctgcgggc
     4501 aggccaccac cgaggtcggc cccttctact gcccggtgga taagaccgtc tatctcgatc
     4561 tcggtttctt cgaccagttg cgctcgcagt tcggcgcgca gggcgggccg ttcgccgaag
     4621 cctacgtgct ggcccacgag tacgggcacc acgtccagga tctggtcggc acgctggcca
     4681 agtaccccaa tccgacgcag ggcgcgcagg gcacctcggt gcgcatcgaa ttgcaggccg
     4741 actgcttcgc cggcgtctgg gccgacaacg ccactcggac cggatttatc acccagctca
     4801 gccaggccga catcgccgat gggctgaacg cggccgccgc cgtgggcgac gatcgcatcc
     4861 agcaggagtt ccaggggcac gtcaaccccg aatcctggac ccacggctcc tcggccgagc
     4921 gccagcactg gttcagcgtc ggctaccaga gcggcgacat gggctcctgc aacaccttca
     4981 gcgggagtct gtgacgcctc acccccgtcc cctctcccag tgcgctggga gaggggcgct
     5041 gcccagcgac gatgttgagt gccgggtcca cacggatccc tcgactgcgc tcgggatgac
     5101 agggaaggat cgcggcgaac gacaacgact tgctgtagtc gtacgccccg gatcaaccct
     5161 gtcatcctga gcttgtcgaa ggatccgtcc tcgactagca cccagccttc gacttcagcc
     5221 gttctcaccc acacggtaca cttcagccat catgacgacg ccctgccctg acccccacca
     5281 cgtccagcgc gtgcgccaga acctcctcga ctggtaccgc gccaaccgcc gcgacctccc
     5341 ctggcggcgg acgcgcgatc ggtatcacat cctggtctcc gaggtgatgc tgcagcagac
     5401 gcaggtcgac cgggtcatcc ccaaatatca cgagttcctg gcgcgcttcc cgacgcttca
     5461 ggcgctggcc gaggcgccca cggccgaggt gatccgcgcc tggtcggggc tgggctacaa
     5521 ccgccgcgcg gtcaacctgc agcgcgcggc gcaggcggtc gtcgagcgcc acggcggggt
     5581 catgccgcgc gagcgggccg cgctggaggc gctccctggc gtggggcgct acaccgccgg
     5641 ggccatcgcc tgcttcgcct acgagcagga cgccggcttc gtcgacacca acattcgccg
     5701 cgtgctgcac cgcgtcttcc acggcccgga gcttccccac gagcaggcga cgccgcgtga
     5761 actggaggcg ctggcggccg cgctggtgcc gcacggcgaa gggtacgact ggaaccaggg
     5821 gctgatggag ctgggcgcgc tgatctgcac ggcgcgccgg ccgctctgcc tcacctgtcc
     5881 gctgcacgac gactgccgcg ccgcgcccgc gattcagacc gtgctggcgg ggctgcccaa
     5941 gggaacccgc cgcaagcagg agggaccctt cgaaggctcg acgcgcttct accgcggccg
     6001 ggtgatccac gccctgcgcg cgctgtcggc gggcgagtcg ctcgacctgg ccagcctggg
     6061 gccgcaggtg cgcgccgact tcggccccga gcacctcccc tggctgcgcg agctagtcgg
     6121 tggcctgacg cgcgacggcc tggccgaggt ggccggggtg gccgaggagc gggcggcata
     6181 cgacgcctcc gcgccggacg agctgcgcgt gcggctgccg tgacggacgc gctcagctca
     6241 tcagcgttgt cgccgcggcc agcgtatcga acggatgcca gaaggtgagg aaggctcggg
     6301 ctaccccctg ccgctcccag cgcgccagcg cgcccgcgat gtcgtcgggc caatcccagg
     6361 cgtaggtcga caggctgacg gaacgcccag ccgcgtcgat ctggtcgcga gcatagccga
     6421 tcaggtcgtc gtcggcgtag acgttgatct catcggcata ggcgaccgcg ctccgaatca
     6481 gccggtgcga gcgcccggcg cccacgatga tgcgtggcgg ctccggcggc agcgtcgcgc
     6541 tggcagcgtc caacaggcgc agctcccgac cctccacgcg caccgcttct ccgctccaga
     6601 gccgccgcag ggcaagcacg gtctcccgca ggccagaaat gcgagcgggc gcgtccggca
     6661 ccgtcatccc ccaggcgcgt gcctccgcga agaaatcgcc cgcgccgatg ccgagctcga
     6721 aacgcccgtc gctcaggacc gccagcgccg ccgcctcccg cgcgaggata cccggcaggt
     6781 agttcagccg gtcgatcacc agcggaacga ggcgaatgcg cgaggtcatg accgccaggg
     6841 cgccgtagag cgaccagccg cagaggtagc gccactccgg attgggagcg tgatagtgat
     6901 cgagcagccc cagcgcgtcg tagccgaggg tatcggcgct ttgggcggcc gcgaggagtg
     6961 ccgcccggtc cgcggtaggg ccaagattca cgccgatgcg cactggtatc ccttccactg
     7021 gcagcgcgga tgcctgtgga tcgccagcat gcggctgccc tgccaggcgc tgctgaatcg
     7081 accgtttaca cggcctccag cgcttcggag accgcatcct ccagcggcag ggcgcggccc
     7141 gccgcccagg cggcttccca ggcgcgctca tcggcacgta agcgcgccgc ggctacctgc
     7201 gcctcgtaat gcggcgcgag attcggcggc acgggcgcgt cgatcgccgc gcgcagggcc
     7261 tcggccgcgc cccagaggcg cgccgcgcgc tcgatcgcgc cccggcccgc cgctgctgcc
     7321 gccagcccct ccaggcagta ggcgatgctc cacttggctc ctagctcctg ggccagtccc
     7381 agactctcgt agaggagcgc cgccgcgcgg gcgtcatcgc cctggcgcag cgtcaggtgg
     7441 cccaggttgc ccagcacgat gaggacgccc cagcgctgct ccagctcgcg catcagcgcg
     7501 agactctcct cgaagagcgc ccgggcttct ccgtcgtcgc cagcatcgcg cgccaccagc
     7561 ccggcgatgt tgagcgcgtc ggcgaccccg ggggcatcgc caagcccgtg cttcagcgcc
     7621 aggctctcgg tcagcagacc gcgcgcccgc gccagctcgc cccgccgtag cgccacctcc
     7681 cccagcgtcg tcagcgcgcg agccacgcct gtcccgtccc ccagggcgcg ggagagggcc
     7741 aggctctcgc cgtagaacgc gtccgcggcc gcgtaatcgc gctgccggat ggccagcgcg
     7801 ccgagaccgt agcaggccgc cgcgcgcaat tcggccggca cgtccgcccc agcggacagg
     7861 ccgcgctcca gccaggcgcg cccctcggcc agatagccgc gcacctccca gaaccaccac
     7921 agcgcgctcg ccaggcgcag cccggtggtc agccgggcgg tgtcgtccgc gcgctcgaag
     7981 gcccagccca gcgccgcccg caggttgtcg tgctcgcgct cgatccggcg cagccagatg
     8041 gcttgctccg agcgcgcgcc ctccgtgaag ctcaagaagt actccgcgtg ccggcgccgc
     8101 agcgcctccc ggtcgtcgct ctccgccagt cgctccgcgg cgtactggcg gatcggctcc
     8161 agcatgcgca ggcgcgggcc gcgctcaccc gctccgtcct cgtggtcgtc cgccatgtcc
     8221 cgcgccagca ggttgttgtt cagcagcgcc atggcccgct ccagcacggc atccgcctcg
     8281 tgcgcatcct gcgcgcagat cgcctcgata gcctccagcg tgcccccgcc ggcaaacagc
     8341 gccagccgcg cgaagagccg ccgctcctcg gcgtcgagca ggtcgtagct ccaggagatg
     8401 gcgtcgcgca tggtgcgttg gcgtgccggg agatcgcggg cgccgccggt ca
//
LOCUS       DASUBI010000703         7769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_986583, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000703 DASUBI010000000
VERSION     DASUBI010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986583"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="pip"
                     /locus_tag="VFU72_13700"
     CDS             <1..884
                     /gene="pip"
                     /locus_tag="VFU72_13700"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEU5424595.1"
                     /translation="WECCGNPQGIPALYLHGGPGSGASPGQRRWFDPTAYKIVLFDQR
                     GAGRSRPLASEPDANLSTNTTQHLIADIEALRRLHDVERWTILGLSWGATLAQAYAHA
                     QPAHVRALVLGAVTTTSRREVEWITEGVGRIFPREWDRFAAAVPPRLRHLRLVDAYAE
                     LLFDPDPAVREQAAREWCAWEDTHVSLTPGHQHNPRYDDPEFRLRFARLVTHYWRHAA
                     FLEEEQLLRDAARLNGIPGVLIHGRYDVSSPLEIAWRLSQRWETSELHVLDDAGHGGG
                     PTFGSVIVDALNRFARE"
     gene            978..2300
                     /locus_tag="VFU72_13705"
     CDS             978..2300
                     /locus_tag="VFU72_13705"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU5424596.1"
                     /translation="MPEDLASWMRDKRLDHLWVAYTDFHGRLAGRDVPLTRVPPVAER
                     GTNFAKANFNFTIRDVQVHNPRFGADAGDVLAVPDPSSAVVLPFARGTGFAFATLHEE
                     DGGVWAGCARSALRRAVAALAGVELQARVAFEPEFYLFHGTGSSAFTSGGMYTIDALA
                     DGMDFVRRLDALLPDMGVSMEQIGKEYGEGQFEANIRPDEPLNAVDDLLLLRLAVRAA
                     AKDLGLHASFMPKVNAAMAGSGLHVHLSLQALDLSDRTADNHPSGLSAEARAFLAGWL
                     EHAEALTALASPTPNSYKRLQPASWAPAHIAWGIGNRAALVRVPGPGRRARLEFRAGD
                     NTSNPYLFLAGLIWAGLDGLQRALEPPAPVTADVGHLAPAELAQRGIRFLPRQAGAAL
                     DALAADETLARGLGELIHGEFLRVKRAELDDYQSQVSDWERASYFDAP"
     gene            2332..3489
                     /locus_tag="VFU72_13710"
     CDS             2332..3489
                     /locus_tag="VFU72_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424597.1"
                     /translation="MTSATLAGQIAEMPFLDHHCHAPLKLPQQLPPEILRATFSETTD
                     RSVQQVDVPQTVAYRAMIRWLAALLGTAPNEEAVLAARAALDPADYQRRLADDARLGP
                     LFDDFLLQPEECYSPEEWSQLTGRPVRTLLRIEVVAQQLIPQAGSWEELRRQFHQALS
                     ESVGRGGVGFKSIAAYRCGLAIEPVDAAQASAALAEVQAELSAGAPNRLVNKTLIDAL
                     VWETLEVAAQLGVPLQFHVGLGDDDVYLPTSNPTLMRSLFQEPRYRHVPIVLLHCYPY
                     VEEAAYLASIYHNAYVDLGLTVPLAGSDCTAQIATAFGLAPTNKLLASSDGHAVPEFQ
                     WFAAHLWRRALSRVLAEVVESDLLTVEQAIESAGLILHGNAERIYPGQGEQ"
     gene            complement(3601..4872)
                     /locus_tag="VFU72_13715"
     CDS             complement(3601..4872)
                     /locus_tag="VFU72_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424598.1"
                     /translation="MISLAAIRSLRYYDFRLIWIAQFVSTLGTQIQTVALAWLVYVIT
                     GSPAALGGIGLARAVPTILLSLFGGTLADQLNRRRLLLVTQCALAVLSALLAVAVSLG
                     LTSIPLFYGFAVITAAAEAFDSPTSQAIIPQLVPREVLSNALTINVIGSDVAAVLGPT
                     VGGLVLGYVGTAAAFWLDAGSFLVVVGALLAMRSRPVTPRLRRGGVAALLDGLHFVRA
                     RGLLWQLMLVDFLATLFVSQSGLLPVFARDVLKVGAQGLGLLYAAPSAGAVIGAAIFA
                     FAPTPRRPGRTVALAIAGYGLVLVLFGLSPTFLVALLFLGLSGALDAVSMAMRHTVRQ
                     LATPNELRGRVGALSGVFSGGGPRLGEFQSGLAASFVGARDAMVFGGVACVAMVLTSR
                     WWGRDLWAYHGEEHPNVAGAPEAKEPVRIGD"
     gene            complement(4991..6889)
                     /locus_tag="VFU72_13720"
     CDS             complement(4991..6889)
                     /locus_tag="VFU72_13720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5424599.1"
                     /translation="MVEADAEHDERGDGLRMVQDVQLDPSGQRLAYVVAAPGLEHREP
                     TARSQIWVAAGEGAPYAATHAQAANIQPRWSPDGATLAFRSDRAERGRMQLYLLRPGW
                     REPELLHEFPSGVCDFAWSPDGTTIAVVATDAAPDRADGADHLLYEDEPRFDRLWLLD
                     AASGETRLLTEEPLHIWEFTWAPDGCSLAAIASERPQRWSWYRARLVRIDTQDGRVET
                     LFTPESQLARPAWSPDGSSIAVIASTWSDPGMTGGDLLLVPLDGDAPRNPTPGAARSY
                     LSLHWQPDGHSVLASALERGQAAICEIGVHGGSETLWRGERAFASHAVSVDREGRRVA
                     AALSGLTEPPEACVGELDGGLSWRQVSVHNAALAGQFAAEVEEVAWPAPDGAESRGLL
                     LRPQGRAGERLPLVTMIHGGPTSAWGYAYPAGGVVAWIPGLLEHGVAVFLPNPRGSTG
                     WGVEYAEANQRDMGGGDLDDILSGIDHLVERGLVDPERLGVCGWSYGGYLTAWAITQT
                     TRFRAAIAGACISNWYSFHGTSDIPGFDEVFYRADPYALDGPYASRSPIFFADRVRTP
                     TLFLHGERDTCCNVGQPMEMFRALRDHGVETQCVIYPREGHPVREDAHRQDMLERGLA
                     WFTRHLGL"
     gene            7078..>7769
                     /locus_tag="VFU72_13725"
     CDS             7078..>7769
                     /locus_tag="VFU72_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5424600.1"
                     /translation="MVTGSGVRVRQRSGAAVVEALQAEGVEHVFGLVGSHVLEIYDAL
                     ADAPSIRHITVKHETAASGMADAYGRLTGRPGVVLVTAGPGATNSLTGVAQAYMAASP
                     LVHISGAVPRGASYESFHGVDQPDFLVRMFAEVTKWSVSVQHPAEIPHVLARAFALAT
                     SGRPGPVHVEIPQDVLLAEATEMDAYQRTEIPPVRLSDDDLARLVAQIRASRNPVIWA
                     GKGVRATRAQQEL"
BASE COUNT         1175 a         2898 c         2644 g         1052 t
ORIGIN      
        1 actgggagtg ctgcggcaac ccgcagggca taccggcgct ctacctgcac ggcgggcccg
       61 gctcgggcgc ttcgccgggt caacggcgct ggttcgaccc cacggcctac aagatcgtgc
      121 tcttcgacca gcgcggcgcc gggcgcagcc gcccgctggc cagcgagccg gacgccaacc
      181 tgagcaccaa caccacccag cacctcatcg cggacatcga agccctgcgc cggctgcacg
      241 atgtcgagcg ctggacgatc ctggggctct cctggggcgc gacgctggcc caggcctacg
      301 cccacgcgca gccggcgcat gtgcgcgcgc tcgtgcttgg cgcggtgacg acgacctcgc
      361 ggcgcgaggt cgagtggatc accgaggggg tcgggcgcat cttcccgcgc gagtgggacc
      421 gcttcgccgc cgccgtgccg ccgcggctgc gccacctgcg gctggtcgac gcctacgccg
      481 agctcctctt cgatcccgat ccggcggtgc gcgagcaggc cgcccgcgag tggtgcgcct
      541 gggaggacac gcacgtctcg ctgactcccg gccaccagca caacccacgc tacgacgacc
      601 ccgagttccg cctgcgcttc gcgcggttgg tcacccacta ctggcggcac gccgccttcc
      661 tggaagagga gcagttgctg cgcgacgcgg cccggctcaa cggtatcccc ggcgtgctga
      721 tccacggccg ctacgacgtc agcagcccgc tggagatcgc ctggcgcctg tctcaacgtt
      781 gggagacgag cgaattgcac gtgctcgacg acgccggcca cggcggaggc cccaccttcg
      841 gcagcgtgat cgtcgacgct ctgaaccgct tcgcgcggga atagacctct cccacagtac
      901 gtcgtcaccg gcacctactt cgttctcgcc gcatcctctg ctaccctagc gcggcgtgcg
      961 caggagagga gcagcgaatg ccggaggatc tggcgagctg gatgcgggac aagcgactgg
     1021 accacctgtg ggtcgcctac accgacttcc acgggcggct ggccgggcgc gatgtgccgc
     1081 tgacgcgcgt gccgcccgtg gccgagcgcg gcaccaactt cgccaaggcc aatttcaact
     1141 tcaccattcg cgatgtacag gtacacaatc cgcgcttcgg cgccgacgcc ggcgacgtgc
     1201 tggccgtccc ggacccttcc agcgccgtgg tcctgccttt cgcgcgcggc accggcttcg
     1261 ccttcgccac gctgcacgag gaagacggcg gcgtctgggc gggctgcgcg cgttccgccc
     1321 tgcgccgcgc ggtggccgcg ctggccggcg ttgaattgca ggctcgcgtg gccttcgagc
     1381 cggagttcta cctttttcac ggcaccggct ccagcgcgtt taccagcggc ggcatgtaca
     1441 ccatcgacgc cctggccgac gggatggatt tcgtgcgccg gctggacgcc ctgctgccag
     1501 acatgggcgt ctccatggag cagatcggca aggagtacgg cgagggacag ttcgaggcca
     1561 acatccggcc cgacgagccg ctcaatgccg tcgacgacct cttactcttg cgcctggccg
     1621 tgcgtgccgc cgccaaggat cttggcctac acgcctcctt tatgcccaag gtcaacgccg
     1681 ccatggccgg ctccggcctg cacgtccacc tcagcctcca ggcgctcgac ctctccgacc
     1741 gcaccgccga caaccacccc agcggcctga gcgccgaagc gcgcgccttc ctggccggct
     1801 ggctggagca cgccgaggcg ctgaccgcgc tggcctcgcc cacgcccaac tcctacaagc
     1861 gcctgcagcc ggcctcctgg gcgccggccc acatcgcctg gggcatcggc aaccgcgccg
     1921 cgctggtccg cgtgccgggg cccggccgcc gcgcccgcct tgagttccgc gccggcgaca
     1981 acaccagcaa cccctatctc ttcctagccg ggctgatctg ggccgggctc gacggcctcc
     2041 agcgggcgct ggagccgccc gcaccagtca ccgccgacgt gggtcacctg gcgcccgccg
     2101 agctggccca gcgcggcatc cgcttcctac cgcgccaggc cggcgccgcg ctcgacgcgc
     2161 tggcggccga cgagacgctg gcgcgcgggc tcggcgagct gatccacggt gagttcctgc
     2221 gcgtcaagcg cgccgagctg gacgactacc agtcgcaagt cagcgactgg gagcgcgcca
     2281 gctacttcga cgcgccctag ctgccatgag cgggcagagg aaggatgtag cgtgaccagc
     2341 gcgaccctgg cgggccagat cgccgagatg ccattcctcg atcaccattg ccacgcgccg
     2401 ctgaagctgc cgcaacagct cccgcccgag atcctgcgcg ccaccttcag cgagacgacc
     2461 gatcgcagcg tgcagcaggt cgacgtgccc cagacggtcg cctaccgcgc catgatccgc
     2521 tggctggccg cgctccttgg caccgcgccg aacgaggagg cggtgctggc tgcccgtgcc
     2581 gcgctcgacc ccgccgacta ccagcgccgc ctggccgacg acgctcgcct cggcccgctc
     2641 ttcgacgact ttctcctgca gcccgaggag tgctactcac ccgaggagtg gtcccaactg
     2701 accgggcgcc cggtgcgcac gctgctgcgc atcgaggtgg tggcccagca gctcattccc
     2761 caggccggct cctgggagga gctgcgccgc cagttccacc aagcgctgag cgagagcgtc
     2821 ggccggggcg gagtcggttt caagagcatc gccgcctacc gctgcgggct ggcgatcgag
     2881 ccggtcgacg cggcccaggc cagcgccgcg ctggctgaag tccaggcaga gctgagtgcc
     2941 ggcgcgccca accggctggt caacaagacg ttgatcgacg cgctggtctg ggagacgctg
     3001 gaggtcgcgg cgcagctcgg cgtgccgctg cagttccacg tcgggctggg cgacgacgac
     3061 gtctacctgc cgacctccaa ccccacgctg atgcgctcgc tcttccagga gccgcgctac
     3121 cgccacgtcc cgatcgtgct gctgcactgc tacccctacg tcgaggaggc ggcctacctg
     3181 gccagcatct accacaatgc ctacgtcgac ctggggctga ccgtgccgct ggccggcagc
     3241 gactgcacgg cgcagatcgc caccgccttt gggctcgcgc ccacgaacaa gctcctggcg
     3301 tcatcggacg gccacgccgt gccggagttc cagtggttcg ccgcccacct ctggcgccgc
     3361 gcgctttccc gggtgctggc cgaagtcgtc gagtccgacc tgctgacggt cgagcaggcc
     3421 atcgagagcg ccggcctgat cctgcacggc aacgccgagc gcatctatcc agggcaggga
     3481 gaacagtaaa cggcgttggt gcccttttac tggattgggc ggcggctgac gacggatccg
     3541 tcgacggatc cgtcgtggca cggcgccgaa cctgttcgtg cgccacgaaa cactctctcg
     3601 tcagtcgccg atccgcaccg gctccttggc ctcgggcgcg ccggccacgt tcgggtgctc
     3661 ctcgccgtgg taggcccaga ggtcgcggcc ccaccagcgc gaggtcagca ccatcgccac
     3721 gcaggccacg ccgccgaaga ccatggcatc gcgcgccccg acgaagctcg ctgccagccc
     3781 gctctggaac tcgcccaggc gcggcccgcc ccccgagaag accccggaca gcgcgccgac
     3841 ccgcccgcgc agctcgttcg gcgtcgcaag ctggcgcacg gtatgacgca tggccatgct
     3901 gacggcatcg agcgcgccgc tgaggccgag aaagagcagg gcgacgagga acgtgggcga
     3961 gagcccgaag aggaccagca ccagcccgta gccggcgatc gccagggcca ccgtgcgccc
     4021 gggccgtcgc ggggtcggcg cgaaggcgaa gatggccgcc ccaatgaccg ccccggcact
     4081 cggcgcggcg tagagcagcc ccaggccctg cgcacccacc ttgagcacgt cgcgcgcgaa
     4141 gaccggtaac aaaccggact gggacacgaa gagcgtcgcc aggaagtcga ccagcatcaa
     4201 ctgccagagc agcccgcgcg cgcgcacgaa gtgcagcccg tcgagcagcg cagccacccc
     4261 accgcgccgc agccggggcg tcaccgggcg gctgcgcatc gccagcagcg cgccgacgac
     4321 caccaggaat gagccggcgt ccagccagaa ggccgccgcg gtgccgacgt agcccagcac
     4381 cagcccgccg actgtcggcc ctaacaccgc cgcgacgtcg ctgccgatga cgttgatcgt
     4441 cagcgcgttg ctcagcacct cacgcggcac gagctgcggg atgatcgcct ggctggtcgg
     4501 gctgtcgaag gcctcggcgg ccgccgtgat gaccgcgaag ccgtagaaga ggggtatgct
     4561 ggtcagcccc agcgagacgg cgaccgccag cagcgccgag aggaccgcca gcgcgcactg
     4621 cgtcaccagg agcaggcgcc gccggttgag ctggtcggcc agcgtgccgc cgaagagcga
     4681 gagcaggatc gtcggcacgg cccgcgccag cccgatgccg cccagcgcgg ccggcgagcc
     4741 ggtgatgacg tagaccagcc aggcgagcgc caccgtctgg atctgcgtac ccagcgtcga
     4801 gacgaactgc gcgatccaga tcagtcggaa atcgtagtag cgcagcgatc ggatcgcggc
     4861 gagggagatc acgcgccctc atcccccccg ccccttctcc caatgctggg agaaggggag
     4921 caagcaacgg acgcggtttc gtcgcccctc tcccagcatt ggaagagggg tagggggtga
     4981 gggctcgtcc tcatagcccc aggtgccgcg tgaaccaggc caggccgcgc tcgagcatgt
     5041 cctgccggtg ggcgtcctcg cgcaccgggt gcccctcgcg cggatagatc acgcactgcg
     5101 tctcgacgcc gtggtcgcgc agggcgcgga acatctccat cggctggccc acgttgcagc
     5161 aggtgtcgcg ctcgccatgc aggaagagcg tgggggtgcg tacgcggtcg gcgaagaaga
     5221 tcggcgagcg gctggcatag ggtccatcga gcgcgtaggg atcggcgcgg taaaagacct
     5281 cgtcgaagcc cgggatgtcg ctggttccgt ggaaggaata ccagttgctg atgcaggcgc
     5341 cggcgatggc ggcgcggaag cgcgtcgtct gggtgatcgc ccaggcggtc aggtagccgc
     5401 cgtagctcca gccacagacg ccgagacgct cggggtcgac cagcccacgc tcgaccagat
     5461 ggtcgatgcc gctcaagatg tcgtccagat cgccgccgcc catatcgcgc tggttggcct
     5521 cggcatactc cactccccag ccggtgctgc cgcgcgggtt gggcaggaag accgccacgc
     5581 cgtgctccaa gagccccgga atccaggcaa ccacgccgcc ggccgggtag gcgtagcccc
     5641 aggccgaggt cggcccgccg tggatcatgg tcaccagcgg cagccgctcc cccgcgcgcc
     5701 cttggggccg caagagcaga ccacggctct ccgcgccatc cggcgccggc caggcgacct
     5761 cttcgacctc ggccgcgaac tgaccggcca gggccgcgtt gtgcacgctc acctggcgcc
     5821 agctcagccc gccgtccagc tcacccacgc aggcttccgg cggctcggtc aggcccgaga
     5881 gcgcggccgc gactcgccgc ccctcacgat cgacgctgac ggcgtgcgag gcgaaagcgc
     5941 gctcgccgcg ccagagcgtc tcgctgccac cgtggacgcc gatctcgcag atggccgcct
     6001 gaccgcgctc cagcgcgctc gccagcacgg agtggccgtc cggctgccag tggagtgaga
     6061 gataggagcg cgccgcaccc ggtgtcgggt tgcgcggcgc gtcgccgtcg agcggcacca
     6121 gcagcaggtc gccaccggtc atgccggggt cgctccaggt gctggcgatc accgcgatgc
     6181 tgctgccgtc cggcgaccag gccggccgcg ccagctgact ctccggcgtg aacagcgttt
     6241 ccacccggcc gtcttgggta tcgatgcgca ccagccgcgc gcggtaccac gaccagcgct
     6301 gcggccgctc gctggcgatc gcggcgagtg agcagccatc cggcgcccag gtgaactccc
     6361 agatatggag gggctcctcg gtcaggagcc gggtctcgcc gctggccgca tccagcagcc
     6421 agagccggtc gaagcgcggc tcgtcctcgt agagcagatg gtcagcaccg tcggcgcggt
     6481 ccggcgcggc gtcggtggcg acgacggcga tcgtcgtgcc gtcgggcgac caggcgaagt
     6541 cgcagactcc ggatgggaac tcatgcagca gctccggctc gcgccagccg ggtcgcagca
     6601 ggtagagctg catccgaccg cgctcggccc ggtcggagcg gaaggcgagt gtggcgccat
     6661 ccggcgacca ccgcggctgg atattggccg cctgggcgtg cgtggccgcg tagggcgcgc
     6721 cctcgccggc ggcgacccag atctgactgc gcgccgtcgg ctcacggtgc tccagccccg
     6781 gcgcggccac cacgtaggct agccgctggc cgctggggtc gagctgtacg tcctgcacca
     6841 tgcgcagacc gtcgccccgc tcgtcgtgct ccgcgtccgc ctccaccatc gccctactct
     6901 ccctctccac ggctccgtgc gtccatcgtc cggtcgataa tctcatcata cgcggcgccc
     6961 cccgtcgata ttccagcgcg ggtcctattt ccgcctgccc ttgcgagcgg tagcaggcac
     7021 ggccatactc tggtcacgac agcggcaggc acatgcatga cagacgagga gcacgcgatg
     7081 gtgacgggga gcggagtccg agtgcggcag cgcagcggcg cggcggtggt cgaggcgctc
     7141 caggccgagg gcgtcgagca cgtcttcggg ctggtcggct cgcacgtgct ggagatctac
     7201 gacgcgctcg ccgacgcgcc ctcgatccgc cacatcacgg tcaagcacga gacagccgcc
     7261 agcggcatgg ccgacgccta cgggcggctg accggccggc ccggcgtggt gctggtcacc
     7321 gccggcccag gcgccaccaa ctcgctgacc ggcgtggccc aggcctacat ggccgcctcg
     7381 ccgctggtgc acatcagcgg cgccgtaccg agaggcgcgt cctacgagtc gttccacggc
     7441 gtcgaccagc ccgacttcct ggtgcgcatg ttcgccgagg tgaccaagtg gagcgtctcg
     7501 gtgcagcatc ccgccgagat cccgcacgtc ctcgcgcgcg ccttcgccct ggcgaccagc
     7561 ggacgcccgg gcccggtcca cgtcgagatt ccgcaggatg tgctgctggc cgaggcgacc
     7621 gagatggacg cctaccagcg gacggagatc ccgcccgtgc gcctcagcga cgacgacctc
     7681 gcccggctgg tggcgcagat ccgcgccagc cgcaacccgg tcatctgggc cggcaagggc
     7741 gtgcgggcca cgcgggcgca gcaggagct
//
LOCUS       DASUBI010000704         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1084627, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000704 DASUBI010000000
VERSION     DASUBI010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084627"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFU72_13730"
     CDS             complement(<1..637)
                     /locus_tag="VFU72_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424601.1"
                     /translation="MSGTWLLDRARLPALLLAGVVLEAALAVGFLWPFSFARLPRPLR
                     PGESLATILGGGWGGALRFVVPALLAFAAYAMAYWLISHSSFRGALAVVLAGTMLFSL
                     TLLPINPVGAQDVYHNIADARTYWVYDQNPTGVPPAHFPNDPLARQVYTWANFPSSYG
                     PLWYQLSGAPLPFTGTVVRANVIGQKALTILFLWGSTVLAALVAEGLRPGTA"
     gene            complement(769..839)
                     /locus_tag="VFU72_13735"
     tRNA            complement(769..839)
                     /locus_tag="VFU72_13735"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Gln,seq:ctg)
     gene            1323..>2221
                     /locus_tag="VFU72_13740"
     CDS             1323..>2221
                     /locus_tag="VFU72_13740"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU5424602.1"
                     /translation="MQILGLDIGKDRLDLALLDDATGVLTDAQHANCPDGIAALVAQL
                     SAWAPELIVLEATGPYHAPVLRVLVAQGLPVAVVNPAQVKAFRQSRLGRSKTDRQDAR
                     LLARFGATYAAELRRYTSPSATQAQLTAWVRYRDSLVAERTRLVSQQEAATWQGDSQV
                     QTWLTERLAQTTQHLAAVDAAIAQLLAELPEAEVLQTVSGVGPRVAAAVLAYVPAALW
                     GNAKAAAAYAGVHPHLEQSGRTSRSWLSKRGPARLRRYLYMAALVAVRHDAAIQAFYD
                     RLRDHGKAPMVALCAVMHKLLRQ"
BASE COUNT          402 a          734 c          763 g          322 t
ORIGIN      
        1 cagccgttcc gggccgcagt ccttcagcca caagcgccgc gagcaccgtg gaaccccaga
       61 ggaagaggat cgtcagcgcc ttctgcccga tgacgttggc gcgcacgacc gttcccgtga
      121 agggaagcgg ggcgcccgag agctgatacc agagcgggcc gtagctggag ggaaagttgg
      181 cccaggtgta gacctgtcgc gctaacgggt cgttcggaaa gtgagcaggc ggcacgccgg
      241 tcggattctg gtcgtagacc cagtaggtgc gcgcgtcggc gatattgtga tagacatcct
      301 gcgcgccgac cggattgatg ggcaggagcg tcagcgagaa cagcatcgtg ccggccagca
      361 ccaccgccag ggcgccgcga aacgaggaat gtgagattag ccagtaggcc attgcgtagg
      421 ctgcgaacgc cagaagtgcc ggcacgacga agcgcagtgc gccgccccag ccgcccccga
      481 ggatcgtcgc cagcgattcc cccggccgca gcggccgcgg cagccgtgcg aagctgaacg
      541 gccacaggaa gccgacggcc agcgccgcct ccagcactac cccggcaagc agcagggccg
      601 gaagccgcgc gcgatcgagg agccatgtcc cgctcaccgc ggtattccct accgtctaga
      661 cgcggccgcg tgcgcgtcgg ctagaggcaa ctgaacggca agaagaaagg gaccgcatgg
      721 ccgggtgcgg tttcgatgaa cgcttctaaa ggaggatgat atcggaggct ggggagcagg
      781 gattcgaacc ccaactggct gatccagagt cagctgtcct accgttagac gactccccaa
      841 agccggccgc gcgcgaccga tgcgcgctaa gtatagcagt atggtgtcgc tccgacaatc
      901 gtcgcctcgg ggccgagcgg cggagtccgg cgaccgggca acgagggcag acacggaggt
      961 ctaccgttac agcaatgacg catctcgact atcctacaac caccgatttt ggatcgtcga
     1021 gtcatcttgc tcgctgtgtc ctacgaaata gatctaaaaa cggcgagtgc aagcctagcc
     1081 ccttgtatct tagagagacc gggggacggc cccgggagca gtggccacgc catggtgggg
     1141 cggcacaccc gcacagcccc tggggccacc acgcccgcac tgaagagggg gttggcgcgc
     1201 agcgggagcg gctcaccgga cagtgtcgag gtctcccatg accgcatcaa caaggctggt
     1261 gcgctcccgc tgcgcgcccc gctccagtat ggcacgacca tgaccgcgct gggagggatg
     1321 cgatgcagat tctcggactg gacatcggca aagatcgcct cgacctggcc ctgctcgacg
     1381 atgcgacggg cgtgctgact gacgcccagc acgccaattg cccggacggg atcgccgccc
     1441 tggtggcgca gctgtcggcg tgggcgccgg agctcatcgt gctggaagcc accggtccct
     1501 accatgcccc cgtgctgcgg gtgctggtcg cccaggggct gccggtcgcc gtggtcaacc
     1561 cggcgcaggt caaagccttt cgtcagagcc ggctggggcg cagcaagacc gaccgccagg
     1621 acgcgcgcct cttggcccgc ttcggggcca cctatgcggc cgagctgcgc cgctacacct
     1681 ccccgagcgc aacccaggcg cagttgacgg cctgggtccg ctaccgcgac agtctggtgg
     1741 ctgagcgcac ccggctcgtg agccagcagg aagcggcgac gtggcagggg gatagccagg
     1801 tgcagacctg gctgaccgag cggctggcgc agacgacaca gcacctggcg gcggtcgatg
     1861 ccgccatcgc gcagttgctg gccgagctgc ccgaggccga ggtgctgcag acggtgagtg
     1921 gggtcgggcc acgcgtcgcc gccgccgtct tggcctatgt gcccgcggcg ttgtggggca
     1981 acgccaaggc cgccgccgcc tatgccgggg tccatcccca tctcgaacag tccggtcgca
     2041 cgagccgcag ctggctgagc aaacggggac cggcccgctt gcgccgctat ctctatatgg
     2101 cggccctggt ggccgtccgt catgatgccg ccatccaggc cttctacgac cgcttacgcg
     2161 accatggcaa agcgccgatg gtggcgctgt gtgcggtgat gcacaagctc ttgcgccaga
     2221 t
//
LOCUS       DASUBI010000705         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1075295, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000705 DASUBI010000000
VERSION     DASUBI010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075295"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFU72_13745"
     CDS             complement(<1..186)
                     /locus_tag="VFU72_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424603.1"
                     /translation="MSLSSRTVNLALLTILVLEFLSGTGSLLVGTSDGRWVFWTHRAG
                     AFALLVLLGWKARIILRS"
     gene            complement(332..529)
                     /locus_tag="VFU72_13750"
     CDS             complement(332..529)
                     /locus_tag="VFU72_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424604.1"
                     /translation="MLSVKSNRRRSRAFPSPIRTTQAKPSEEPRLPLRPAVAHLNRID
                     GPGFAAPLHDSPLPALVRPLV"
     gene            959..2419
                     /locus_tag="VFU72_13755"
     CDS             959..2419
                     /locus_tag="VFU72_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5424605.1"
                     /translation="MARPRRQPVAMAAAPAPALRRRRRSLKRAAIIGVPLLLLALVAA
                     YVVPLALRAHDAAGKIFVPPPPRIAAAATGASTAPPVATRNAQGTPVPPPQSYATTAP
                     IAMPSWDKKERINILLLGADSNAARRNEGEPPLSDTIIIMTIDPATKTVGMLSIPRDL
                     YVNIPGIGSDKINSAYSNGELSNITGPGLVQATIEQNFGITIHYFVEIDLEGFQKMVD
                     TLGGVTLDVTAPLKDDEYPGQSYNYTRILYHTGLQHMDGATALSYVRSRHDDSDFGRG
                     ARQQAFLKAFREQAVSLNLITKAPQLISNLGDTVRTDLSPTDVLKLAQLATSIPNGNI
                     HSYNLLDATSVQYSPGQPYYLIPDWDKIHAILGQMMPGGGGTPGAAPTVQTPDLSARV
                     LVLNGTHVDKLAARASSKLSEIGFQSVSVAQAPDAGQHPTSIIYDVSGDTPTSRLVAQ
                     TLGLPSSSIQKADAAQLGNNQIVVVLGDDAPPKVGN"
     gene            2506..3486
                     /locus_tag="VFU72_13760"
     CDS             2506..3486
                     /locus_tag="VFU72_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU5424606.1"
                     /translation="MLRRSGWGWLGGALLLLSLLAACGQPDAGLTRAGAAPSGRILFA
                     QKGDISLWQGGKLQQVTHLGDASAPSWAPDGQRFVFVRTGDGWSDLFVGQLGGDQYQQ
                     LTHDRPNGQIGTQAFVDNASWALDPDWSPSGDTIAYVSDLGTQLNFLWLIQGLGNQPR
                     QVAASTSNQQLVEGPSFSPDGSKIVYTQRVQPDSSIDRTTELWMVDLESGKATRLVQG
                     GNGSYDPAWSPDGRWIAFVQRTGQQNDLWLVSAKGGTAVKLTSGPQVVAPAWAPDSSS
                     IAFFTPDGEGFDADYISFSTDANGNPQVTQPQTLFKTDGVDAPSGMSWTK"
BASE COUNT          642 a         1316 c         1221 g          556 t
ORIGIN      
        1 agagcggaga atgatgcgag ccttccagcc caggaggacg agcaacgcga aggcgccggc
       61 gcggtgggtc cagaagaccc agcggccatc cgaggtgccg accagcaggc tgccggtccc
      121 ggagaggaac tcaagcacga ggatcgtcag cagcgccagg ttgaccgtac ggctggatag
      181 agacatcgcg agcgacctcc ggcgccgtgg acgccacgcc gccggccacg actatacggg
      241 gcgcgccccg tccggcgcaa accggcgcgc aaaggcaccg gccatccgct cggatggccg
      301 ggaccacagg ttgggaaggt ggggctgaac gtcaaacgag cgggcgcacc agcgccggta
      361 gcgggctatc gtgcagggga gcggcgaagc cgggcccgtc gatgcggttg aggtgcgcca
      421 ccgcgggccg caggggcagc cgcggctcct cggacggttt cgcctgggtg gtgcgaatcg
      481 ggcttgggaa tgctctgctg cgacgtcggt tcgacttgac ggagagcatg ctcccccctt
      541 cctgccgtgc tgtagctcct gcctggctcc ttcgcaatat ccttgccact ttcgcttctg
      601 tgcctcttcg gcacccctca atctccgcgg gcaccttgca tctcgcgtcc ggaatttgta
      661 aacaggtgta cgagcacacg cggtcgctcc agcagctgtc gtcgtcggtg ctccggcgcc
      721 cggtggtccc gtgctgacct gtcacctttt ggggccgccg cgcgttaata cggtttgggc
      781 aacctgagat ggtcagagga ggcatgggct gagccagcgg cgcaagcgac acctgggggc
      841 gcaccctcaa taccagccgc gcggtcgtca gcaggccggc gacgtcaggg tccgccggct
      901 ttcggccgag gaaattcgcc gttcccggca agggggtggg gcggcaggac cgcccgcgat
      961 ggctcgccca cggcggcagc cggtggcgat ggccgcggca cctgctcccg ctcttcggcg
     1021 ccggcgccgc tcactcaagc gcgcggccat catcggcgtc ccgctcctgc tgctggcgct
     1081 ggtggcagcc tatgtcgtgc cgctggcgct gcgggctcac gacgcggccg gcaagatctt
     1141 cgtgccgccg ccgccccgca tcgccgccgc cgcgaccggc gccagcacgg cgccgcccgt
     1201 cgccacgcgc aacgctcagg gaaccccggt gccgccgcca cagtcctacg ccaccaccgc
     1261 gccgatcgcc atgccgagct gggacaagaa ggagcgcatc aacatcctgc tgctcggggc
     1321 cgactccaac gccgcgcggc gcaacgaggg tgagccgccg ctctccgaca ccatcatcat
     1381 catgaccatc gaccccgcga ccaagacggt cggcatgctc tcgatcccgc gcgatctcta
     1441 cgtcaatatc cccggcatcg gcagtgacaa gatcaactcc gcctactcca acggcgagct
     1501 gagcaacatc accgggccgg ggctggtgca ggccaccatc gagcagaact tcggcatcac
     1561 catccactac ttcgtcgaga tcgacctcga aggcttccag aagatggtcg acacgctcgg
     1621 cggcgtgacg ctcgacgtga ccgcgcccct caaggatgac gagtatcctg gtcagtcgta
     1681 caactacacc cgcatcctct accacaccgg gctgcagcac atggatggcg ccaccgcgct
     1741 cagctacgtc cgctcgcgcc acgacgacag cgacttcggc cgcggcgcgc gccagcaggc
     1801 gttcctcaag gcgttccgcg agcaggccgt ctcgctgaac ctgatcacca aggcgccgca
     1861 gttgatctcg aacctgggcg atacggtgcg taccgacctc tcgccgaccg acgtgctcaa
     1921 gctggcgcaa ctggcgacct cgatccccaa cggcaacatc cactcctata acttgctcga
     1981 cgctaccagc gtgcagtaca gccccggcca gccctattac ctgatcccgg attgggacaa
     2041 gatccatgcg atcctggggc agatgatgcc gggtggcggc gggacgccag gtgccgcgcc
     2101 gaccgtgcag acgccggatc tctcggcccg agtgctggtg ctcaatggca cccatgtcga
     2161 caagctggcg gcgcgtgcct cctcgaagct gagtgagatc ggcttccaga gcgtgtcggt
     2221 ggcgcaggcg ccggatgccg ggcagcaccc gacctcgatc atctatgatg taagtggcga
     2281 cacgccgacc tcgcgcctgg tggcacagac cctcggccta cccagctcgt ccattcaaaa
     2341 ggccgatgcc gcgcaactgg gcaacaatca aatcgtggtg gtgctgggcg acgacgcgcc
     2401 gccgaaggtg ggtaactagg agaccaagat gacgagcaag ccgcgacgcg gcgtcccggc
     2461 cgccgggctg atgggttggt gccgatgatc ccgggcgcga gacacatgct ccggcgctcc
     2521 ggctggggct ggctcggcgg cgcgctgctc ctgctctcgc tgctggccgc ctgtggacag
     2581 cccgacgccg gcctgacgcg cgccggggcc gcgcccagcg ggcgcatcct cttcgcgcag
     2641 aagggcgata tctcgctctg gcaagggggc aagcttcagc aggtgacgca cctgggcgac
     2701 gcctccgcgc cgagctgggc gccggatggg cagcgcttcg tcttcgtgcg caccggcgac
     2761 ggctggtcgg acctcttcgt cggccagctc ggcggcgacc agtaccagca gctgacccat
     2821 gaccgcccga atgggcagat cggcacgcag gcgttcgtcg acaacgccag ttgggcgctc
     2881 gacccggatt ggtccccctc gggcgatacc atcgcctacg tcagcgacct cggcacccag
     2941 ctgaacttcc tctggctgat ccaggggctg ggcaatcagc cgcggcaggt ggcggcctcg
     3001 acctcaaatc agcagctcgt cgaggggccg tcgttctcgc ccgacggcag caagatcgtc
     3061 tacacccagc gcgtgcagcc ggacagcagc atcgatcgca ccaccgagct ctggatggtc
     3121 gatctggaga gcggcaaggc gacgcggctg gtgcagggcg gcaacggcag ctacgatccg
     3181 gcctggtccc ccgatggccg ctggatcgcc ttcgtgcaac gcaccggcca gcagaatgac
     3241 ctctggctgg tctcggccaa gggcgggacg gcggtgaagc taaccagcgg gccacaggtg
     3301 gtggcgccgg cctgggcgcc tgatagctcg tccattgcct tcttcacgcc ggacggcgag
     3361 ggcttcgacg ccgactacat cagcttctcg accgacgcca acggcaaccc gcaggtgacg
     3421 caaccgcaga cgctcttcaa gaccgacggc gtcgacgcgc cgtcggggat gtcgtggacg
     3481 aagtaggggt gacgggcatc atccgcggac tcgcccttcg atcgagaatc ggggcgtgtg
     3541 aggagagatc cttcgacaag ctcaggatga cagggatagg cagcagccta gcggacgagc
     3601 gaggccgtcg cccaagcccc atcctcctcg gagtcccctc acgaaccctg tcatcctgag
     3661 cgcagtcgaa ggatccgtcc tcacacgtca caaaacctcg tcgacgaaca gcctctaccc
     3721 cagcacccgc acccc
//
LOCUS       DASUBI010000706         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_26291, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000706 DASUBI010000000
VERSION     DASUBI010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26291"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFU72_13765"
     CDS             complement(<1..387)
                     /locus_tag="VFU72_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424607.1"
                     /translation="MDQEDNVTQRRIAKRLVLDVSYSVGRQDPANGVFEGGVPGPEHM
                     DEFRIDAASDAVDSERTDELAERSRLELNVRGSRRAYRAFAGYLLALCELETQDSDYH
                     DHFDEVRSTTGEPVIHMIVHRPVDRHS"
     gene            complement(470..2080)
                     /locus_tag="VFU72_13770"
     CDS             complement(470..2080)
                     /locus_tag="VFU72_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5424608.1"
                     /translation="MSAGDRHEPRGAEVLVIGSGMAGAAIAARLSAHGVRVVCLEQGP
                     WVLPAEHPHYSNEWEFALQRDWHRSPAKRQAERDYPISSNRVDVMLYNAVGGATTHYN
                     AHWPRLRPSDFRKGTEHGLAGAADWPLSYEELAPYYAQNDHEQGVAGLAGDPAVPPRE
                     PRQTRPHPFGPHGKKIAAGLERLGWHWWPADNAILSEDYDGRVGCIQCGSCRNGCPRG
                     SLAMVTRTYWPKALRNGVELRADARVERITVDEQGRADGAVYIDLRSGERYRQRAEKV
                     VLAANGLGSPRLLLLSASGRCPNGLANSSGLVGHHLMHHAYAENTVVFDEPIDGFAGA
                     YGAPIFCQEFAETDVNRGFVNGFTFQIGRGMTASPAALGLPWGAEHRAAFAKFFNHEL
                     WFSVQGEDLPVFENRVELDHEHVDSSGLPGVKLFWELHPNDQRLVAYGVERARELAEA
                     AGAVEFRTSGAANPNPAWHLLGTCRMGSDPETSVVDAEHQAWDVPNLYICDGSSFVTG
                     GAVNPTSTIGALALRCADRILARAHSGF"
     gene            complement(2077..2625)
                     /locus_tag="VFU72_13775"
     CDS             complement(2077..2625)
                     /locus_tag="VFU72_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424609.1"
                     /translation="MAEPGWTLTGDERRTLALALDALLPPGEEGEGSFPWPSQTGLID
                     EFILRRVPPAGVARVPYPGLDSDGLRRALRPLAPAGDLPGMTAALARLEAEDQDSFTG
                     LWRLAVYGYYSRPETIAAIQRELAPAYHGAPLPLGYAHAIEPWNPDDPLQHPRHPRGH
                     YVPTEAVRRVDVDSLSFEEGRR"
     gene            3341..3754
                     /locus_tag="VFU72_13780"
     CDS             3341..3754
                     /locus_tag="VFU72_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424610.1"
                     /translation="MRHRLRLLLVILGFTLVISVLGIAPAFAAKGSGSHTGGGGGKHG
                     GGTSSTATLLISPASPTANSTIAVSGCGYLVNVPAEIGYVTPTAATVGGITVDSNGCF
                     ATSLYVSGSGSYSIGVQQYVNNSWVVEASIAFTVQ"
BASE COUNT          590 a         1322 c         1352 g          615 t
ORIGIN      
        1 tgagtggcga tctaccgggc gatggacaat catatgaatc actggttcgc cagttgtact
       61 acggacttca tcgaaatggt catggtagtc agaatcctgc gtctcgagtt cacagagggc
      121 gagtagatag cctgcgaacg cacggtaggc gcgccgagat cctcgtacat tcagctcgag
      181 tctactgcgc tcagcgagct catccgttcg ctcgctgtcg acggcatccg acgccgcgtc
      241 tattcggaac tcatccatgt gttccggacc aggcacgccg ccctcaaaga ccccatttgc
      301 gggatcctgc cgacccactg agtaggagac atcgagcacc agccgcttcg ctatgcgtcg
      361 ctgggtaaca ttgtcttcct gatccatggg ctgcattcgc ctccaacgcg gctaatccag
      421 aatcacgtgt cctggagttc ggaactgctc cacggttcgt atccgcggat caaaagcccg
      481 agtgcgcgcg cgccaggatg cggtcggcgc agcgcagggc cagggcgccg atggtcgagg
      541 tggggttgac cgcgccgccg gtgacgaacg acgagccgtc gcagatgtag agattcggca
      601 catcccaagc ctgatgctcg gcgtcgacca ccgacgtctc cggatcactg cccatgcggc
      661 aggtgccgag caggtgccag gccgggttcg gattggccgc gccgctggtg cgaaactcca
      721 ccgcgcccgc cgcctcggcc agctcacgcg cgcgctcgac accgtaggcg accagccgct
      781 ggtcattggg gtgcagctcc cagaagagct tgacgccggg caggccggag gagtcgacgt
      841 gttcgtgatc cagctcgacg cggttctcga agaccggcag atcctcgccc tgcacgctga
      901 accagagctc gtggttgaag aacttcgcga aggccgcgcg gtgctccgcg ccccagggga
      961 gacccagcgc ggccggcgag gcggtcatgc cccggccgat ctggaaggtg aagccgttga
     1021 cgaagccgcg gttgacgtcg gtctcggcga actcctgaca gaagatcggc gcgccgtagg
     1081 ccccggcgaa gccgtcgatc ggctcgtcga agacgaccgt gttctcggcg taggcgtggt
     1141 gcatcaggtg gtggccgacc aggccggagc tgttcgccag gccgttgggg cagcggccgc
     1201 tcgccgagag gaggagcagg cgcggcgagc cgaggccgtt ggcggccagc accaccttct
     1261 cggcgcgctg gcggtagcgc tcgccgctgc gcaggtcgat gtagaccgcg ccgtcggccc
     1321 gcccctgctc gtcgacggtg atccgctcga cccgcgcgtc ggcgcgcagc tccacgccgt
     1381 tgcgcagcgc cttgggccag taggtgcgcg tgaccatcgc cagcgagccg cgcgggcagc
     1441 cgttgcggca ggagccgcac tggatgcagc cgacgcgccc gtcgtagtcc tcactgagga
     1501 tggcgttgtc cgccggccac cagtgccagc cgagccgctc cagcccggcg gcgatcttct
     1561 tgccgtgcgg cccgaacggg tgcgggcgcg tctggcgcgg ctcgcgcggc ggcacggcgg
     1621 gatcgcccgc caggccggcg actccctgct cgtggtcgtt ctgcgcgtag tagggcgcca
     1681 gttcctcata gctgagcggc cagtcggcgg cgccagccag cccgtgctcg gttcccttgc
     1741 gaaagtcgga ggggcgcagc cgcggccagt gggcgttgta gtgcgtggtg gcgccgccga
     1801 cggcgttgta gagcatgacg tcgacgcgat tggaggagat ggggtagtcg cgctcggcct
     1861 ggcgcttggc cgggctgcgg tgccagtcgc gctgcagcgc gaactcccac tcgttgctgt
     1921 agtgcgggtg ctcggcgggg agcacccacg gtccctgctc caggcagacg acgcgcacgc
     1981 cgtgcgcgct caggcgggcc gcgatggccg ccccggccat gcccgagccg atcaccagca
     2041 cctcggcccc gcgcggctcg tgcctgtccc cagcgctcat cgtcgcccct cctcgaacga
     2101 caagctatcg acatcgacgc gccgcacggc ctcggtcggc acgtagtggc cgcgcgggtg
     2161 ccggggatgc tgcagcgggt cgtcggggtt ccagggctcg atggcatgcg cgtagcccag
     2221 cggcagcggc gcgccgtggt aggccggcgc cagctcgcgc tggatggcgg cgatcgtctc
     2281 ggggcgcgaa tagtagccgt agaccgccag gcgccagagc ccggtgaagg agtcctggtc
     2341 ctcggcctcc agccgcgcca gcgccgccgt catgccggga agatcgccgg ccggcgcgag
     2401 cgggcgcagc gcccgccgca gaccgtcgct gtcgaggccg gggtagggca cgcgcgcgac
     2461 gccggctggc ggcacgcgcc gcaggatgaa ctcgtcgatc agccctgtct gggagggcca
     2521 ggggaagctc ccctccccct cctccccggg cggcagcaac gcgtccagcg ccaatgccag
     2581 cgtacgccgc tcgtcgccgg tgagggtcca gcccggttcg gccatcccga cctccgtgtt
     2641 cccgaggaca tcactcccta gcggattgca tggcgagatt atagcgcgtg cgcctgcgta
     2701 tccggcaccg acgcgccagt cgggcggatt cattgcgtga caagcgagca ccggcgtagt
     2761 acagtcgcgg tggagaagca gcggtcatct cccgcgagtg ggaggagaag ccgagcgtga
     2821 cgcgcgcacg attggcgctc gcccgttcgc cgcggaattt ggcccgctga tcttccgaag
     2881 aagatggctc gtcgggcgtt ccagcccggg caggtgccgg cgctctgctg tcttggggaa
     2941 gcagggagcg cactggaacg cacggctcgc agccaagggg acgcggtcaa acgcgtagga
     3001 agggtgattg ggggccagcg caggtgagct gcgacggcaa ccttgcctgg gggaacactc
     3061 cattcctgga caattcgcgc gcccgacgcg cccgccgcga gtcatcgccc gacaaggcca
     3121 cacggctgga tccggcgtgc cttcgccggc acgtcaggcc gcggctggac gctgtctgat
     3181 ccggcgccgc tggcgaaggt acgccggaag tcctggcctg aatcccccac cctgcatcac
     3241 gcatttggcc ctatctggcg gctgtgccca gccgcgtgag gcgagatgat cgccgcgtgg
     3301 atatgggcaa tctggacggg cattcgtgaa agggattcgc atgcgtcatc gtcttcgctt
     3361 gctgctcgtg atcctggggt tcacgctcgt catctcggtg ctcggcatcg cgccggcctt
     3421 cgcggccaag gggagcggca gccataccgg cggcgggggc ggcaaacacg ggggcggcac
     3481 cagctccacc gcgaccctgc tgatctctcc cgcatctccg acggccaaca gcacgatcgc
     3541 ggtctccggc tgcggctacc tggtcaacgt tccggccgag atcggctacg tgacgcctac
     3601 ggccgcaacg gtcggcggca tcactgtcga cagcaacggc tgcttcgcca ccagcctcta
     3661 cgtctccggt tcgggcagct actcaatcgg cgtccagcag tacgtgaata actcctgggt
     3721 ggtggaagcg agcatcgcat tcaccgtcca gtaaggtgct acgcacttcg aattgactcg
     3781 aagcccgcca gtggcgggct tcgctgttct cggggacgca atctcggccc ccactccggc
     3841 cccggcgacg ccggacactg gcgccgctcc ttggcctgg
//
LOCUS       DASUBI010000707         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_264458, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000707 DASUBI010000000
VERSION     DASUBI010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264458"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFU72_13785"
     CDS             <1..324
                     /locus_tag="VFU72_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424611.1"
                     /translation="TTWSGVRRARQELTLLTARIAAGDYGEVRAIHGVSLFAEAGKRL
                     GFEFRPLPHTWRWALERYFMIGLLIIYHPEGWRRARQLPETIWPGEVWLGLAGPRRPD
                     AVRPL"
     gene            complement(488..1357)
                     /locus_tag="VFU72_13790"
     CDS             complement(488..1357)
                     /locus_tag="VFU72_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424612.1"
                     /translation="MAGTRRGFGVAAAIAPVVIRAAASSSESYGYDSFWVNDTAQGDG
                     LRALAEAAADTAIIKLGVGVIPLSRRSPESISAQVNDLAPKIGQSGHVGDTIEMSGQL
                     HTIHGVSLPLDRLLLGVGSGAGGPGALERVRRGVELLRAQLDTRIFISALGPRMSRLA
                     GEVADGVLFNWLTPDYARRSAEWVREGAARAGRDVPQLYAYVRVALGPASTARLVEES
                     ARYGAIPSYADHFRRMAVEPAATGIGVKTPEEIRALLAPWEGAVDEVVIRAITAEDTL
                     AETIDLIKAAAPL"
     gene            1480..2088
                     /locus_tag="VFU72_13795"
     CDS             1480..2088
                     /locus_tag="VFU72_13795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5424613.1"
                     /translation="MPAVVPVLPGERVRLVPFGPEHLPLAGRWRADPEVTRYWISDEA
                     PNPAELDAWYQENQASGALTWAILDERSTPIGYTNLFDLDAANRHAELALMIGERAAW
                     GRGYATDALRTLLQRAFAPAGNEDKEGEGEGLNLHKVSLTVFAENLAARRVYAACGFR
                     EDGVVRDDIYRAGRWHDQVLMSALATEFTGRASQHGLGRQAE"
     gene            2085..2396
                     /locus_tag="VFU72_13800"
     CDS             2085..2396
                     /locus_tag="VFU72_13800"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEU5424614.1"
                     /translation="MIVAIVTFRYPAETFDPDRFVQQSRERRVAYEGLPGLILKIYWT
                     AEQGPEAGGIYLWDSMERAQATYNEEWRRRAGQAFGVEPEIRYLAVSDTIQNLPIPPL
                     P"
     gene            complement(2388..>2670)
                     /locus_tag="VFU72_13805"
     CDS             complement(2388..>2670)
                     /locus_tag="VFU72_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424615.1"
                     /translation="CGLAVSVLCDALGRPYLVTVRGTRLRLATARLLSWLGAAASGLL
                     GWVVLIHLFPELLALATFFGLEAVLLDVCAHRILYLPVSVPLRVRQPSR"
BASE COUNT          413 a          962 c          940 g          355 t
ORIGIN      
        1 accacctgga gcggcgtgcg ccgggcccgc caggaactga cgctcctgac cgcgcgcatc
       61 gcggcgggcg actacggcga ggtgcgtgcc atccacggcg tcagcctctt cgccgaggcg
      121 ggcaagcggc tcggcttcga gttccggccg ctgccgcaca cctggcgctg ggcgctggag
      181 cgctacttca tgatcgggct gctgatcatc taccaccccg agggctggcg ccgggcccgg
      241 cagttgccgg agacgatctg gcccggcgag gtctggctgg ggctcgccgg cccgcgccgg
      301 cccgatgccg tacggccgtt gtaggagcag gccctcatgg ctccttgccg gccctgcacg
      361 tccgccgcca gcccacattc gccccatccc cctggcaggc gcggggcgac ggggcgggct
      421 ccgcgcctgc cgcaatggcg accggcgcac gacgcacgac gctcagccac agtgcggcgc
      481 cgccgtgtca cagcggcgcc gcggccttga tcagatcgat cgtctcagcc agcgtgtcct
      541 cggcggtgat ggcgcggatg actacttcgt cgaccgcgcc ctcccagggc gccagcagcg
      601 cgcgaatctc ctccggcgtc ttgaccccga tgccggtcgc cgccggctcg accgccatac
      661 ggcggaagtg atcggcgtag gaggggatgg cgccgtagcg cgccgactcc tctaccagcc
      721 gcgcggtgct ggccgggccg agcgccacgc gcacgtaggc gtagagctgg gggacgtcgc
      781 gtccggctcg cgccgcgccc tcgcgcaccc actcggccga gcggcgtgcg tagtcgggcg
      841 tcagccagtt gaagagaacc ccgtcggcga cctcgccggc caggcgcgac atgcgcggcc
      901 ccagcgccga gatgaagatg cgcgtgtcga gctgcgcccg cagcagctcc accccccggc
      961 gtacccgctc cagcgcgccc ggtccgcccg cgccgctgcc gacgccgagc agcagccggt
     1021 cgagcggcag gctgacgccg tgaatggtgt ggagctgccc gctcatctcg atcgtgtcgc
     1081 cgacgtgtcc actctggccg atcttcggcg cgagatcgtt gacctgcgcg ctgatgctct
     1141 ccggcgagcg gcgcgagagc gggatcacac cgaccccgag cttgatgatg gcggtgtcgg
     1201 ccgccgcctc ggccagcgcg cgcaagccat caccctgcgc cgtgtcgttg acccagaagg
     1261 agtcgtaacc gtacgactcc gatgatgaag cggccgcgcg gatcaccacc ggcgcgatgg
     1321 ccgccgcgac cccgaagcct cgccgtgttc ccgccatctc tgctccctcc acctccagct
     1381 ccgctggcgc acggccacaa tgcgccgcgc caatgatcgc ataaactctg cataaggctt
     1441 gccgggtggc ggcgacacgt cacgggaaag gagcggcgta tgccggcggt ggtgccggtc
     1501 ttgcctgggg agcgcgtgcg gctggtgccg ttcggcccgg agcatctgcc gctggccggc
     1561 cgctggcgcg ccgatccgga ggtcacgcgc tactggatca gcgacgaggc gccgaacccg
     1621 gccgagctgg acgcctggta ccaggagaac caggccagcg gcgcgctgac ctgggccatc
     1681 ctcgacgagc ggagcacgcc gatcggctat accaacctct tcgacctcga cgccgccaac
     1741 cgccacgccg agctggcgct gatgatcggc gagcgtgccg cctggggccg gggctacgcc
     1801 acggacgcac tgcggacgct gctccagcgc gctttcgcgc cggcggggaa tgaggacaag
     1861 gagggtgagg gagaagggct caacttgcac aaggtgtcgt tgactgtctt cgcggagaac
     1921 ctggcagcac ggcgcgtcta cgccgcctgc ggctttcgcg aagacggagt cgtgcgcgat
     1981 gatatctacc gcgcgggccg ctggcacgac caggtattga tgagcgccct ggcgacggag
     2041 ttcaccgggc gcgcgagcca acatggacta gggaggcaag ccgaatgatc gtcgcgatcg
     2101 tcaccttccg ctaccccgca gagaccttcg atcccgaccg cttcgtgcag cagtcgcgcg
     2161 agcgccgcgt cgcctatgag gggctgcccg gcctgatcct caagatctac tggacggccg
     2221 agcagggccc cgaggcgggt gggatctacc tgtgggactc gatggagcgg gcgcaggcga
     2281 cctacaacga ggagtggcgc cgccgcgcgg ggcaggcatt cggcgtcgaa cccgaaatcc
     2341 gctacctggc ggtcagcgac accatccaga acctgccgat tccgccgcta ccgtgagggc
     2401 tgccgcaccc gcagcggcac actcaccggc aggtagagga tacggtgggc gcagacatcg
     2461 agcagcacgg cctcgaggcc gaagaaggtc gccagcgcca gcagctcggg gaaaaggtgg
     2521 atcagcacca cccagccgag caggccgctg gccgccgctc ccagccaact gagcaggcgg
     2581 gctgtggcga ggcgcaggcg cgtgccgcgc acggtgacga ggtagggccg gcccagcgca
     2641 tcgcagagca cggagaccgc caacccacag
//
LOCUS       DASUBI010000708         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_798872, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000708 DASUBI010000000
VERSION     DASUBI010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798872"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="VFU72_13810"
     CDS             complement(<1..2148)
                     /locus_tag="VFU72_13810"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU5424616.1"
                     /translation="MAWLFGLTLFTSAALLFIAQPLVAKSLLPLWGGSPSVWNTCMVF
                     FQAALLLGYAYAHAATAWLGARRQALLHLALILAPLAFLPFAVPAEPPASEHPAAALL
                     GYLVAAVGLPFVAVATTAPLLQAWFASTGDRDPYPLYAASNLGSLLALAAYPLVLEPN
                     LGLRQQRTIWACGYGLLALLIVVCAANVWRKCAARATLTPSPLVGEGWGGGSATPATM
                     PEREPPAARRPSLTLPHEGGGAQKPADLTRHRLGPRIFARWVALAFVPSSWLLGVATY
                     ITSDIAPVPLLWVVPLALYLLSFILVFANSTRQVDAWAARLLPLLAFPLALVLGLGLA
                     QAFWLPLHLLTFCAGALVCHGALARDRPDPQHLTAFYLALALGGVLGGLFNALIAPSL
                     FDRVAEYPLALVLGCLALPAVPPRRTLWPVLSRHSAWLVPLAIFGLIALLVRNVGGIE
                     PTPLGTTLEVLASGLVVFVWYTHRGRPKRFALGIGAALLASGLADGISGRTLLRERNF
                     FGTVRVTVADTPGGRFHRLFHGNTLHGQQALEPSRRGEPLAYFHRTSPIAQVFAVHRA
                     PGRVAIVGLGAGAMAAYAREHERWSFYELDPAVIRIARDPRYFTFLSDSRAGRIDVIE
                     GDARLRLREAPERAFELIVLDAFSSDAVPAHLLTREALALYRHQLAAGGQIVFNITNR
                     YLDLEPVVGALARDAGMVCRVCYDVHVTDEQKAA"
BASE COUNT          408 a          795 c          823 g          281 t
ORIGIN      
        1 cgcggctttt tgctcgtcgg ttacgtgcac gtcgtaacac acgcggcaaa ccatgccggc
       61 gtcgcgtgcc aaggccccga cgaccggctc gaggtcgagg tagcggttcg tgatgttgaa
      121 gacgatctgg cccccggccg cgagttggtg gcgatagagc gcgagcgcct cgcgcgtcag
      181 caggtgcgcg ggcacggcgt cggagctgaa ggcgtcgagc acgatcagct cgaacgcacg
      241 ctccggggcc tcgcgcaggc ggaggcgggc gtcgccctcg atcacgtcga tgcggccggc
      301 ccggctgtcg gacaggaacg tgaaataacg cgggtcgcgc gcgatccgga tcacggccgg
      361 gtcgagctcg taaaagctcc agcgctcgtg ctcacgcgcg taagccgcca tcgcgccggc
      421 gccgagcccg acgatcgcga cgcggccggg cgcgcggtgt acggcgaaga cctgggcgat
      481 cgggcttgtg cgatgaaaat aggccagcgg ctcgccgcgc cggctcggtt cgagcgcctg
      541 ctgaccgtgg agcgtgttgc cgtggaacag gcggtggaat cggccgcccg gcgtgtcggc
      601 cacggtgacg cgcaccgtgc cgaagaagtt gcgctcgcgc agcaaggtgc gcccgctgat
      661 cccgtcggcg aggccgctcg ccagcagcgc cgccccgatc cccagcgcga agcgcttcgg
      721 ccgcccgcgg tgcgtgtacc aaacgaacac gacgagcccg gacgccagca cctcgagcgt
      781 cgtccccagc ggcgtcggct cgatgccgcc cacgttgcgc accagcagcg cgatcagccc
      841 gaagatggcc agcgggacaa gccacgccga gtgccggctg agcaccggcc agagggtgcg
      901 gcgtggcggc acggccggca acgccaggca gcccagcacg agcgcgagcg gatactccgc
      961 gacgcggtcg aagagagaag gggcgatgag cgcgttgaac agcccgccca gcacgccgcc
     1021 gagcgccagc gcgaggtaga acgcggtgag gtgttgcggg tcggggcggt cgcgcgccag
     1081 cgctccgtgg cagaccagcg cgccggcgca gaacgtcagc aagtgcagcg gcagccagaa
     1141 cgcttgcgcc agccccagcc ccaacaccaa cgccagcgga aacgccagca gcggcaagag
     1201 ccgcgcggcc caggcgtcga cttggcgggt tgaattggcg aacaccagga tgaacgacag
     1261 caggtaaagg gccagcggca cgacccaaag cagcgggacg ggcgcgatgt cactcgtgat
     1321 gtaagtcgcg acgccgagca gccagctcga cggcacgaac gcgagcgcga cccagcgggc
     1381 gaagatgcgc ggtccaaggc ggtgcctcgt aaggtctgcc ggtttctgcg cccctccccc
     1441 ctcgtggggg agggtcaggg aagggcggcg cgccgccggt ggctctcgct ccggcattgt
     1501 cgcaggtgtc gccgaccccc ctccccaacc ctcccccaca aggggggagg gagtaagagt
     1561 tgcacgcgcg gcgcatttgc gccacacgtt cgccgcgcaa actacgatga gcagagccaa
     1621 caacccgtag ccgcacgccc agattgtccg ctgttgccgc agccccaggt ttggttcgag
     1681 caccagggga tacgccgcca gcgcgagcag gctgccgagg ttgctcgccg cgtagagcgg
     1741 gtacgggtcg cggtcacccg tgcttgcgaa ccacgcctgg agcaaagggg ccgtcgtcgc
     1801 caccgcgacg aacggcaagc cgaccgcggc gacgaggtat cccagcagcg ccgcggccgg
     1861 gtgctccgag gcgggcggct cggccgggac tgcgaacggc aggaacgcca agggagccag
     1921 aatcaacgcc aggtgcagca atgcctgccg ccgtgcgccg agccacgcag tcgcggcgtg
     1981 cgcgtacgcg tagccgagca atagcgcggc ctggaagaag accatgcacg tgttccagac
     2041 cgacggcgag ccgccccaca gcggcaggag cgatttcgcg accaggggtt gtgcgatgaa
     2101 cagcagagcc gcgcttgtga agagcgttag accaaacagc caggccaaat cgagcctccc
     2161 caacgagccg ttgcggtcgg ccccattttc gagcccccgc ctctttcgcc gtgtctccgt
     2221 cgttcttacg ataacggcgt ccggcgccgg gtgctcgcgc ctcggctcct tcacgcacct
     2281 tgactcggag acgccgaccg atggcac
//
LOCUS       DASUBI010000709         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_634826, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000709 DASUBI010000000
VERSION     DASUBI010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634826"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU72_13815"
     CDS             <1..274
                     /locus_tag="VFU72_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEU5424617.1"
                     /translation="LVHKDSRTYYNEELTWRIIALAERAGIPYQHAVWEFFGSDGDAL
                     IQQGIPAALIGLSTRYTHSPFETFDLRDFDAALRLVTAFVTTPARG"
     gene            324..1166
                     /locus_tag="VFU72_13820"
     CDS             324..1166
                     /locus_tag="VFU72_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5424618.1"
                     /translation="MSDLIQRLNGLCDEQPFHTGWYFKDLKSGQTADRAGATVVPSAS
                     TRKIAILMAALREVHRGRFSLDQPFTIEARYQNTTSGIFQHFTPGSSFPLRDALVGMI
                     VVSDNTCTGKIADMIGLDVINEFSRSVGMVGTTHRHSTPPGNLGFDHPVEATNATTPS
                     DVGRLLELILQGTQDASAAEKLGSTTELVQLGLDILSWQKLNQRMPYLLPGGVKVAHK
                     TGTGARNFNDAGLVFAGDEPRFILTVYTDGVPATLPDGRAGHSAADLLIAHLTRACWD
                     ALCA"
     gene            complement(1297..2106)
                     /locus_tag="VFU72_13825"
     CDS             complement(1297..2106)
                     /locus_tag="VFU72_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEU5424619.1"
                     /translation="MVMDVATAAIRQEEPGAVRIAARSGELHGITAGRASGYAQANLA
                     ILPRELAYDFLLFCQRNPKPCPLLEVTDTGSPEPRLTAPGADLRTDLPRYRVYRHGEL
                     VDEPAEISDLWRDDLVAFLLGCSYTFEAPLVEAGVPVRHLSCGRNVPMYRTNRACVPA
                     GVFRGPLVVSMRAIPAGQLVRAVQVTSRFPAVHGAPVHLGDPAALGIADIAQPDWGDP
                     PSMEPGDVPVFWACGVTPQAVAMAARPPLMITHAPGHMFVTDLPIGSLAVL"
     gene            2239..2781
                     /locus_tag="VFU72_13830"
     CDS             2239..2781
                     /locus_tag="VFU72_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5424620.1"
                     /translation="MSGSVVAVSRSGGHTLRKPNQESIELQVGLGVAGDAHNGVTVKH
                     RSRVARDPTQPNLRQVHLIHEELHDELRGRGFDVAPGVMGENVTTRGVDLLGLPTGTR
                     LRLGNDAVIEITGLRNPCAQLDEIQPGLMKAVLDRDADGNLIRKAGVMAIVLTGGEVR
                     PGDPITVELPVAPHQPLAPV"
     gene            2797..>3121
                     /locus_tag="VFU72_13835"
     CDS             2797..>3121
                     /locus_tag="VFU72_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5424621.1"
                     /translation="MDLLDRLLGHDAWTTEQLLRLSRQLGDEQLDRRFDLGNQSLRAT
                     FAHMIGNVEVWTGLMAGRALDPSPEPVSLDELARRHARSYAEFAALARRIRDQRRWDD
                     RYLDTL"
BASE COUNT          514 a         1123 c         1062 g          422 t
ORIGIN      
        1 gctggtgcac aaggacagcc gcacctacta caacgaggag ctgacctggc gcatcatcgc
       61 gctggccgag cgcgccggca tcccctacca gcacgcggtc tgggagttct tcggcagcga
      121 cggcgacgcg ctgatccagc agggcatccc ggccgcgttg atcggcctct cgacccgcta
      181 cacccactcc cccttcgaga ccttcgacct gcgcgacttc gatgccgccc tgcggctggt
      241 cacggccttc gtgaccaccc ccgcgcgcgg gtgagcggcg ctatgatgga gcggcgagca
      301 gcctagcgaa gaggagcagc gacgtgagcg atctcatcca gcggctcaac gggctctgcg
      361 acgagcagcc cttccacacc ggctggtatt tcaaggacct caagagcggc cagacggccg
      421 accgcgcggg cgccacggtg gtcccctcgg ccagcacgcg caagatcgcc atcctgatgg
      481 cggcgctgcg cgaggtccac cgcgggcgct tctcgctcga tcagcccttc accatcgagg
      541 ccaggtacca gaacacgacc tccggcatct tccagcactt cacgccaggc agctcgttcc
      601 cgctgcgcga cgcgctggtc ggcatgatcg tggtcagcga caacacctgc acgggcaaga
      661 tcgccgacat gatcggcctc gacgtgatca acgagttctc gcgctcggtc ggcatggtgg
      721 gcaccaccca ccgccacagc actccgccgg gcaacctggg cttcgaccac ccggtcgagg
      781 cgaccaacgc caccacgccc agcgacgtcg gccgcttgct cgaattgatc ctgcagggca
      841 cgcaagacgc cagtgcggcc gagaagctgg gcagcacgac ggaactggtg caactgggac
      901 tcgacattct gagctggcag aagctgaacc agcgcatgcc ctacctgctg ccggggggcg
      961 tcaaggtggc gcacaagacc ggcaccgggg cgcgcaactt caacgacgcc ggcctcgtct
     1021 tcgccggcga cgagccccgc ttcattctga cggtatacac cgacggcgtg ccggccacgc
     1081 tgcccgacgg ccgcgccggc cactcggccg ccgacctgct gatcgcccac ctgacacgcg
     1141 cctgctggga cgcgctctgc gcctgaaggg aagcgaaggg ctgacgagaa cgtcgcgtcg
     1201 tcctcgacag cccctctcta accctgtcat cccgagcgga gtcgagggat ccgtgccgac
     1261 gcggcaccca acgtcatcga acggcaggcc gccgccctac agcaccgcca gcgagccgat
     1321 cggcagatcc gtcacgaaca tgtggccggg ggcatgggtg atcatcagcg gcgggcgcgc
     1381 ggccatggcg accgcctgcg gcgtcactcc acaggcccag aagaccggca cgtcgcccgg
     1441 ctccatgctg ggcgggtcgc cccaatcggg ctgggcgata tccgcgatgc cgagcgccgc
     1501 tggatcgccg aggtggaccg gcgcgccgtg gaccgccggg aagcgcgagg tcacctgcac
     1561 cgcacgcacc agttgaccgg ccgggatggc gcgcatcgag acgaccagcg gcccgcggaa
     1621 gacgccggcc ggcacgcagg cgcggttggt gcggtacatc ggcacgttcc gcccgcacga
     1681 cagatgccgc accggcacgc ccgcctcgac cagcggtgcc tcgaaggtgt aggagcaccc
     1741 gagcagaaag gcaaccagat cgtcgcgcca gagatcgctg atctcggccg gctcgtcgac
     1801 cagctcgccg tgacggtaga cgcggtagcg cggcaggtcg gtgcgcagat cggcgccggg
     1861 cgcggtgagc cttggctcgg gcgacccggt gtcggtcacc tccagcagcg ggcagggctt
     1921 ggggttgcgc tggcagaaca gcaggaagtc gtaggccaac tcgcgcggca ggatcgccag
     1981 attcgcctgc gcgtagccgc tggcccggcc cgcggtgata ccgtgcaact cgcccgagcg
     2041 ggcggcgatg cgcaccgcgc ccggctcctc ctgccggatg gcggctgtgg cgacatccat
     2101 gaccatcgct cgctcccttc gtgctcgcgg cttccacgcg acttcagtgt agcgcgccgc
     2161 gcgtgccgcg ccagccatca cgccgcaaat cagctatgat ggcggcgacg atccggccgc
     2221 aaagtgaggg actgacgcat gagtgggagt gtggtggcgg tcagccgcag cggtgggcac
     2281 accctgcgca agccgaacca ggagtcgatc gagttgcagg tcgggctggg cgtggccggc
     2341 gacgcgcaca acggtgtcac ggtcaagcac cgttcgcggg tggcgcgcga cccaacccag
     2401 ccgaacctgc gccaggtcca cctgatccac gaggagctgc acgacgagct gcgcgggcgc
     2461 ggcttcgacg tggcgcccgg ggtgatgggt gagaacgtca ccacgcgcgg cgtcgacctg
     2521 ctcggcctgc ccactggcac gcggctgcga ctggggaatg acgcggtgat cgagatcact
     2581 gggctgcgca acccgtgcgc gcaactggac gagatccagc ccggcttgat gaaggccgtg
     2641 ctcgatcgcg acgccgacgg caatctgatc cgtaaggccg gcgtgatggc gatcgtgctg
     2701 accggcggcg aggtccgccc cggcgacccg atcaccgtcg agctgccggt cgcgccccac
     2761 cagccgctgg cgccggtcta gcgcggaaag ctgcacgtgg acctactcga tcgcttgctg
     2821 gggcatgacg cctggaccac ggagcaactg ctgcggctca gccggcagct cggcgacgag
     2881 cagctcgacc gccggttcga cctgggcaac cagtcgctgc gcgccacctt cgcccacatg
     2941 atcggcaacg tcgaggtctg gaccggcctg atggcgggcc gcgcccttga cccgtcccct
     3001 gagccggtct cgctcgacga gctggcgcgg cggcacgcgc gctcgtacgc cgagttcgcg
     3061 gccctggcgc ggcggattcg cgaccagcgg cgctgggacg accgctatct cgataccctc
     3121 g
//
LOCUS       DASUBI010000710         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_522834, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000710 DASUBI010000000
VERSION     DASUBI010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522834"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..786
                     /locus_tag="VFU72_13840"
     CDS             352..786
                     /locus_tag="VFU72_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5424622.1"
                     /translation="MPIYEYFCKDCRKRVSVFFRSYAAVGDPVCPECGGTKLERRFSR
                     VVVRRGSGPQPESASEEDWGGDDFGGYPSDAEFGGGFGDDFGLDEDADPRELARWARQ
                     MSAEMGEPLDLELDSALADMERGADPDEVMSRLEESDPEPEG"
     gene            complement(811..993)
                     /locus_tag="VFU72_13845"
     CDS             complement(811..993)
                     /locus_tag="VFU72_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424623.1"
                     /translation="MGISKNAFVLPFTAGTALGVFGGLVLGAMLGHRLATLVVHAWTL
                     LVGDDDDEPRFELLLQ"
     gene            complement(1027..1374)
                     /gene="rsfS"
                     /locus_tag="VFU72_13850"
     CDS             complement(1027..1374)
                     /gene="rsfS"
                     /locus_tag="VFU72_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5424624.1"
                     /translation="MTNATALAREIASIASDSHATDIQVLDIHSLSPIADFFVLASAD
                     NTRLLRTIEQRIRETLRDEGLRPLRVEGLPEHGWIVIDYGDVIVHLLTIEQREYYRLE
                     DLWSEAHRLLVIQ"
     gene            complement(1450..1524)
                     /locus_tag="VFU72_13855"
     tRNA            complement(1450..1524)
                     /locus_tag="VFU72_13855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1490..1492),aa:Ile,seq:cat)
     gene            complement(1542..>2707)
                     /locus_tag="VFU72_13860"
     CDS             complement(1542..>2707)
                     /locus_tag="VFU72_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424625.1"
                     /translation="DTALYIYNRLVAKNEVGGHPEMPGVTPAEFHDFVLRRQHAWPRS
                     LNASSTHDTKRGEDVRARLVVLSELAGEWEGRLDHWAALNGDKRAIVGEQPAPDGNTE
                     LLIYQTLLGAWPLEEADMPAFPRRVKEYLVKAAREAKTHTGWTAPNEAYEAALAAFVD
                     RVLDPVLSRDFLADFRPFQERLAFWGALTSLSQLLIKLTAPGVPDIYQGNELWALNLV
                     DPDNRRAVDYSRRERLLRELSTSRPPDLATLLHEWRDGRIKLWLTRSLLALRAAYPAL
                     FRDGDYLPVEAEGEGARHILTYLRHQGDVWVLVVAPRLVAGLTLPEEFPLGEVWSDTV
                     LRLPNGAPAAWDSPLTGERLVGGGGHAVPMARLLARAPVGLWLAGVEARMPLA"
BASE COUNT          428 a          880 c          948 g          451 t
ORIGIN      
        1 tgcgcgcggt cggcgaccag cagacgctag cgcgggtgcg cgccgaggtg gcggagttgg
       61 tggcgccgtt cccgttgccc ggcgaagcgg ccgcggcgcg agcgggaacg gcggggtagg
      121 gcggaggtca tgctctatcc cgcggctttc gtgccgagcg gcgcgcggct ccgaccgggt
      181 tgaaaaccac cgcgccagat ggttacgaaa gcctaatgaa gcaggctgag acggatcagc
      241 ccttcgcctg cttctcgcag gcttcgtctc ctagcgcggt ggcttgagcc ccgcgctgtt
      301 gccgcatgcg gacgtgtggt tcgttggccg cccatgatcg aggaagcagt tgtgccgatc
      361 tacgagtatt tctgtaaaga ctgccgcaag cgggtcagcg tcttcttccg ctcgtacgcc
      421 gcggtgggcg atccggtctg tccggagtgc ggcggcacca agctcgagcg gcgcttttcg
      481 cgggtggtgg tgcgtcgggg tagtggcccg cagccggaga gcgcgagcga agaggactgg
      541 ggcggcgacg atttcggcgg ctatccgagc gatgcggagt tcggcggggg cttcggcgac
      601 gatttcgggc tggatgagga tgccgatccg cgggagctcg cgcgctgggc gcggcagatg
      661 agcgccgaga tgggcgagcc cctcgatctc gagctggata gcgcgctggc cgacatggag
      721 cgcggcgcgg acccggacga agtcatgagc cggctggaag aatccgatcc ggagccggaa
      781 ggttagcggc tctggcgcgg agcgcccgcg ctactgcagc agcagttcga agcgcggctc
      841 gtcgtcgtcg tcacccacga gcagcgtcca ggcgtgcacc accagcgtgg ccaggcggtg
      901 gcccagcatg gcgcccagca cgagtccgcc gaagactccg agcgcggtcc cggcggtgaa
      961 gggaaggaca aacgcgttct tgctgatccc catggcgttc cctgcctgat gcttacccgg
     1021 tcgatcctac tgaatgacca gcagccggtg agcctcggac cagaggtctt cgagccggta
     1081 atactcgcgc tgctcgatcg tcagcaggtg gacgatcacg tcaccgtaat cgatcacgat
     1141 ccatccatgc tccggcaatc cctcgactct cagtggccgc agcccctcgt cgcgcaacgt
     1201 ctcgcggatt cgctgctcga tcgtacgcag caaccgtgtg ttgtcagcgg acgcgagcac
     1261 gaagaaatcc gcgatcggtg agaggctgtg gatgtcgagc acctgaatgt ccgtcgcgtg
     1321 ggaatcggat gcgatacttg cgatctcgcg cgcgagcgcg gtggcgttgg tcattgatct
     1381 cctgtccgga cttcctgtac gtacaccaag tatacacctc tccgccgcat tctggcaatg
     1441 ggcgggtgct ggtgggcctg gtaggattcg aacctacgac cttgaggtta tgagcccctt
     1501 gctctgcccc tgagctacag gcccgacggc aggcccctat cttatgccag cggcatccga
     1561 gcttcaacgc ctgccagcca gagcccaacc ggcgctcgtg ccagcaaccg cgccatcggc
     1621 accgcatgtc cgccgccacc gacgagccgt tcaccggtca gaggtgagtc ccaggcggcg
     1681 ggcgccccgt tgggcaacct caagacggta tccgaccaga cctcccccag cgggaactcc
     1741 tcgggcaatg tcagcccagc taccagccgc ggcgcgacta ccagcaccca gacgtcaccc
     1801 tggtggcgca ggtaggtcag gatgtgccgc gcgccttcgc cttcggcctc gaccggcagg
     1861 tagtcgccgt cgcgaaacag cgccgggtag gccgcgcgta gcgccagcag gctccgcgta
     1921 agccagagct tgatgcggcc gtcgcgccac tcgtgcagca gggtggccag gtccgggggg
     1981 cgcgacgtcg acagctcgcg cagcaggcgc tcgcgccggg agtagtcgac cgcgcggcgg
     2041 ttgtccgggt cgaccaggtt gagcgcccac agctcgttcc cctggtagat gtcaggcacg
     2101 ccgggcgcgg tcagcttgat caggagctgg ctgagcgagg tcagggcgcc ccagaaggcg
     2161 agccgctcct gaaaggggcg aaagtcggcc aggaaatccc gcgagaggac ggggtcgagc
     2221 actcggtcca cgaaggcggc cagcgccgct tcgtaggcct cgttcggcgc ggtccatccc
     2281 gtatgtgtct tggcttcgcg cgccgctttc accagatact ccttgacccg cctggggaag
     2341 gcgggcatat ccgcctcctc cagcggccaa gcgccgagca gcgtctgata gatcaagagc
     2401 tcggtgttgc cgtcgggcgc gggctgctct ccgacgatgg cgcgcttgtc gccgttgagc
     2461 gccgcccagt ggtcgagccg gccctcccac tccccggcga gctcggacag taccaccaga
     2521 cgggcgcgca catcctcgcc gcgcttcgta tcgtgggtgg agctcgcgtt caggctccgc
     2581 ggccaggcgt gctggcgccg gagcacgaag tcgtggaact cggccggggt gacgccgggc
     2641 atctccgggt ggccgccaac ctcgttcttg gcgacgagcc ggttgtagat gtatagggcg
     2701 gtgtctt
//
LOCUS       DASUBI010000711         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_283394, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000711 DASUBI010000000
VERSION     DASUBI010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283394"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU72_13865"
     CDS             <1..274
                     /locus_tag="VFU72_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424626.1"
                     /translation="ILRGAHGSDEELAELWLAWQERHLYAMRRAVESLVARDALRPGL
                     APDEAVDVLYALAGTEVYRSLVRERGWAPERYEDWLFRTIRTELVG"
     gene            400..1155
                     /locus_tag="VFU72_13870"
     CDS             400..1155
                     /locus_tag="VFU72_13870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5424627.1"
                     /translation="MADKAHFSDFDQRGYRTVDARTGYGEWVSSYERTVEDAMDLALL
                     ERLTTVPWSTRQRAADLGCGTGRTGRWLRRHGVPAIDGVDVTPEMLAVARSSGVYERL
                     VEGDVADSGLPSKAYELVTVCLVDEHLAELDPLYREAFRLAAPGAFLVLVAFHPHFIM
                     VSGMPTHYTSVSGEPVAIETHVHLLSDHVSAALAAGWALAELQERLIDDEWLALKPQW
                     RSYRGYPISAGFVWCMRDPPAAQPPGRGTETST"
     gene            complement(1119..>2610)
                     /locus_tag="VFU72_13875"
     CDS             complement(1119..>2610)
                     /locus_tag="VFU72_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU5424628.1"
                     /translation="VHLWEDGTEALRGSRTPDIVIVFMYALFFVPVIYASLNFGRAGA
                     IPTAIWTAILALPSILFFHHGSDRWAEVLQHLTIIALAAVIATRVDREIAARHEAEQE
                     GQARRLSEVKYRALFASAHDAIVVFSESGYVEEANAAAAELLSQPGGIAPGTPLSALV
                     GAEQAAYLSAFASSGEVGGEDLRLTPRPGIERWVEPVWSAVRVAGGRPAIQVVFHDVT
                     ERRSAQQGLEAYARQIVAAQEEERKRIARDLHDGSLQSVVLLCRQIDQVESHAGELFE
                     GLRRATSELRRIEEAGQAARRHRLLDGAATERALTTERLATIEASVAATASALAGEIE
                     TVHRSAETIADELRGFSWSLRPSILDDLGLVPAVEWLVHDLEDRTGTRGDFEVTGVER
                     RLPAALEPGLFRIAQEALHNVERHAAAREVRVRLDFDLAAIHLSVADNGSGFPVQEGH
                     TTSSLTGKLGLRGMRERARLLGGTVHITSTAGRGTQVDVSVPLPGG"
BASE COUNT          362 a          957 c          895 g          396 t
ORIGIN      
        1 tatcctgcgc ggcgcgcacg gcagcgacga ggaattggcc gagctgtggc tggcgtggca
       61 ggagcgccac ctctacgcca tgcggcgggc ggtcgagagc ctggtcgccc gcgatgcgct
      121 gcgtcccggg ctggcccccg acgaggcggt cgacgtgctc tacgcgctag ccggcaccga
      181 ggtctaccgc tccctggtgc gcgagcgcgg ctgggcgccg gagcgctacg aggattggct
      241 cttccgcacg atccgcacgg agcttgtcgg ctgatcgcga gcttcaccgg cgcggctggc
      301 gcaccccttc acgagcggcg ccctgaaggt tgacccgccc gcggccgggc gaggcgtcac
      361 cggcttgcgg ccggcgagcg gagcgaccat actccgatca tggcggacaa ggcgcatttc
      421 tccgacttcg accagcgcgg ctaccgcacg gtggacgcgc gcaccggcta cggcgaatgg
      481 gtctccagct acgagcgcac cgtcgaggat gccatggacc tcgcgctgct cgagcgactc
      541 accacggtac cgtggtccac gcgccagcgt gcggccgatc ttgggtgcgg gaccggccgc
      601 accggtcgct ggctgcgccg ccacggtgtc cccgcgattg acggcgtcga tgtcacgcca
      661 gagatgctgg cggtggcacg ctcgagcggt gtctacgagc gactggtcga gggggatgtc
      721 gctgattcgg ggctgccgag taaggcctat gagctcgtca ccgtctgtct ggtcgacgag
      781 cacctggcgg agctcgaccc gctctatcgc gaagccttcc gcctggcggc gccgggcgcg
      841 ttcctggtgc tggtcgcctt ccacccgcac ttcatcatgg tctcgggcat gccgacacac
      901 tacaccagtg tcagcggcga gccggtcgcc atcgagacac acgtccatct actcagcgac
      961 cacgtgtcgg ctgccctcgc cgccggctgg gcactggccg aactgcagga gcgcctcatc
     1021 gacgacgagt ggctggccct caagccgcag tggcgctcct accgcggcta tccgatcagc
     1081 gccggcttcg tctggtgcat gcgtgacccg cccgccgctc agccaccggg aaggggaacc
     1141 gagacgtcga cctgagtgcc ccggccggcg gttgaggtga tgtgcaccgt gcccccgagc
     1201 aggcgggctc gctcgcgcat gcctcgcagg ccgagcttgc cggtcaggga gctggtggtg
     1261 tggccttcct gcacggggaa accgctgccg ttgtcggcga cgctcagatg gatcgcggcc
     1321 aggtcgaagt ccaggcgcac gcgcacctcg cgagcggccg cgtgccgctc cacgttgtgc
     1381 agcgcctctt gggcgatgcg gaagagccct ggttcaagcg ctgccgggag ccgtcgctcg
     1441 acgccggtta cctcgaagtc gccgcgcgta cccgtgcgat cctccaggtc atgcaccagc
     1501 cactcgaccg ccggcaccag tcccagatcg tcgaggatcg acgggcgcag gctccaactg
     1561 aagccgcgca actcgtcggc gatggtctcc gccgaccggt gcaccgtttc gatctcgccc
     1621 gccagtgccg acgcggtcgc ggcgacgctc gcctcgatcg tcgcgagccg ctcggttgtc
     1681 agcgcacgct cggtggccgc cccgtcgagc agccggtgcc tccgcgctgc ctggcccgcc
     1741 tcctcgatgc gccgcagctc gctcgtcgcc cgccgcaggc cttcgaagag ctcgccggcg
     1801 tgggactcca cctggtcgat ctgccggcag agcagcacga ccgactgcag gctgccgtcg
     1861 tggaggtcgc gcgcgatccg tttgcgctcc tcctcctgag cggccacgat ctgccgcgcg
     1921 taggcctcca gcccctgctg cgcgctccgc cgctcggtca cgtcgtggaa gacaacctgg
     1981 atcgcgggcc ggccgcccgc cacccgcacc gcgctccaga ccggctcgac ccagcgctcg
     2041 atccccggcc ggggcgtgag gcgcaggtcc tcgccgccga cctccccgct ggaggcgaac
     2101 gcgctcaggt aggcggcctg ctcggctcca accagcgcgc tcagcggcgt ccccggggcg
     2161 atgccacctg gctgggacag cagctcagcc gccgcggcgt tcgcctcctc gacgtacccc
     2221 gattcgctga agacgacgat cgcatcgtgc gcgctggcga agagcgcgcg gtacttgacc
     2281 tccgagagcc ggcgtgcctg cccctcctgc tccgcctcgt gccgcgcggc gatctcgcga
     2341 tcgacccggg tcgcgatgac ggccgccagc gcgatgatcg tcaggtgctg cagcacctcg
     2401 gcccagcgat cgctgccgtg gtggaagaag aggatgctcg gcagcgccaa gatcgcggtc
     2461 cagatcgcgg tcgggatcgc accggcgcgg ccgaagttca ggctggcgta gatgaccggc
     2521 acgaagaaga gtgcgtacat aaagacgatg acgatgtcag gtgtccggct gccccgcagc
     2581 gcctccgtcc cgtcctccca gagatgcacc
//
LOCUS       DASUBI010000712         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_100805, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000712 DASUBI010000000
VERSION     DASUBI010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100805"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="VFU72_13880"
     CDS             <1..2073
                     /locus_tag="VFU72_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5424629.1"
                     /translation="IIRPEPLNGARGLIIDRKNRPLAMNRISWSVSLVAAQVPDDPQE
                     VEYLRQQLISTLGLKDQLVVRKSGLPLDSEGAVLAGLAGAIGSDPSKLTHAVLNSPDS
                     LVVVKSDLSPSDAARLAGLNKQFPGTHVMNWLDYILELHAGDATPIVISKDVPREVAL
                     ALQDNALYLPGVQVSDQQLHREYPAGQDFSHVLGYLGPVSEDEYKKSRAEADSHGDQV
                     GTYLSTDDVGRGGLEEALEQELRGIHGLRWVQADARGVEIAELYSRRQDAKPGLSVQI
                     SLDVEFQKAVTAALQEGIAKANDAALKAQKDPVGAGVAIAINPQTGELLALVSLPTYD
                     NQKFVDGISEADYQAYLNNPFKPLTDFAVSGEFPPGSPIKPLLSCAALTEGPGNGGLD
                     AGMEYHCAGSIRVPDTTDEAGGHTYVCWNHAGHGSLDLRHALSQSCDIFFYNVGAPHQ
                     EDQFTREPLHYYEPDGSKHYFQGLGIDKIGKYLKEQFGFGQPTGIELAGEASGRVPDA
                     KWMFQTLHEYWSVGDTINVSIGQGQLSCTPLQLLCAIAAIANGGTYYQPRLVQRLIRS
                     NGHVEREYAPVTVRDLAFSDEHIAIVREGMRMTVADPKGTAYGKFVLTGNDFPIAGKT
                     GTAEFGVAENGVYEKSHAWFTCFAPFEKPEIAVVVLIQGGGEGATFAVPVADAILAAY
                     FGKTPPKS"
     gene            2157..>2697
                     /locus_tag="VFU72_13885"
     CDS             2157..>2697
                     /locus_tag="VFU72_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEU5424630.1"
                     /translation="MVQSRQGGHEQVKVRGTRDGLILQLPTHLPFADLLGQVRASVGS
                     GGEFFRSGEIILDYAERTPNVEEIVALKGLLAERGIRLRTVVAGRQASRDLLRSWGYH
                     PLRLVDAGDEVREVAVGAADERTALYVRRTLRSGASIGADGDIVVLGDVNAGAEVQAG
                     GDVLVWGAIRGTVHAGVNGD"
BASE COUNT          462 a          868 c          946 g          421 t
ORIGIN      
        1 atcatccgtc cagagccgct caacggcgcg cgcggcttga tcatcgaccg caagaaccgg
       61 ccgctggcca tgaaccgcat tagctggagc gtgtcgctgg tggcggcgca ggtcccggat
      121 gatccccagg aggtcgagta cctgcgccag cagttgattt cgaccctcgg tctaaaggat
      181 caactggtgg tgcgcaagag cgggctgccg ctcgactcgg agggcgcggt gctggccggg
      241 ctggccgggg cgatcggcag cgatcccagc aaactgacac acgcggtgct caactccccc
      301 gactccctgg tcgtggtcaa gtccgatctc agcccgtcgg acgccgcgcg cctcgccggt
      361 ctcaacaagc agttccccgg cacgcacgtg atgaattggc tggactatat cctggagctg
      421 cacgcggggg acgccacgcc gatcgtgatc agcaaggatg tcccgcgtga ggtcgccctc
      481 gcgctgcagg acaacgcgct ctacctgccg ggcgtccagg tcagcgatca gcaactgcac
      541 cgcgagtatc ccgccggaca ggacttctcg cacgtgctcg gctatctcgg accggtcagc
      601 gaggacgagt ataagaagtc gcgcgccgag gcggatagcc acggcgacca ggtaggcacc
      661 tacctctcga ccgacgatgt cgggcgcggc gggttggagg aggcgctcga gcaagagttg
      721 cgcggcatcc acggcctgcg ctgggtgcag gccgatgcgc gcggggtgga gatcgccgag
      781 ctctacagcc ggcggcagga cgcgaagccg gggctctcgg tgcagatcag tctggacgtg
      841 gagttccaga aagcggtgac ggccgcgctg caggagggga tcgccaaagc gaacgatgcc
      901 gcgctcaaag cacagaaaga cccagtgggg gccggtgtgg ccatcgcgat caacccgcag
      961 acgggcgagt tgctggcgct ggtcagcctc ccaacctacg acaaccagaa gttcgtcgac
     1021 ggcatcagcg aggccgatta ccaggcgtac ctcaacaacc ccttcaagcc gctgaccgac
     1081 ttcgccgtca gcggcgagtt cccgcctggc tcgccgatca agccgcttct ctcctgcgcc
     1141 gccctcaccg agggtccggg caacggcggc ctcgacgccg gcatggagta ccactgcgcc
     1201 ggctcgatcc gcgtgcccga caccaccgac gaggcgggcg gccacaccta cgtctgctgg
     1261 aatcacgcgg gccacggctc gctcgacctg cgccacgcgc tctcgcagtc gtgcgatatc
     1321 ttcttctaca acgtcggcgc gccgcaccag gaagaccagt tcacccgaga gccgctgcac
     1381 tactacgagc cggacggctc caagcactac ttccaggggc tcggcatcga caagatcggg
     1441 aagtatctga aggagcagtt cggcttcggt cagccgaccg ggatcgagct ggccggcgag
     1501 gccagcgggc gcgtgcccga cgccaaatgg atgttccaaa cattgcacga atactggtcc
     1561 gtcggcgata ctatcaacgt ctcgatcggt caggggcagc tctcctgcac gccgctgcag
     1621 ttgctctgtg cgatcgccgc gatcgccaac gggggcacct actatcagcc gcggctggtg
     1681 cagcgcctga tccgctccaa cggtcacgtg gagcgggagt acgcgccggt cacggttcgc
     1741 gatttggcgt tcagtgacga gcacatcgcc atcgtgcgtg aggggatgcg catgacggtg
     1801 gcggacccga aggggacggc gtacggcaag ttcgtgctga ccggcaacga cttcccaatc
     1861 gccggcaaga ccggcactgc ggagttcggg gtggccgaaa acggcgtgta cgagaagtcg
     1921 cacgcctggt ttacttgttt cgcgccgttc gagaagccgg agatcgcggt cgtggtcttg
     1981 atccagggcg gtggcgaggg cgcaaccttc gcggtgccgg tggcagacgc catcctggcg
     2041 gcatacttcg gtaagacgcc gccgaagtcg taggggtcgg ggaaccgggg atcgggttcg
     2101 gcctcgccgc cgcggtgcgc cgcgtggcga ggcggcgggt gaaggagcga agtcgcatgg
     2161 tgcagtcgcg ccagggcggg cacgagcagg tgaaggtgcg ggggacccgc gacgggttga
     2221 tcctgcaact ccccacgcat ctgccgtttg ccgatctgct gggccaggtg cgcgccagcg
     2281 tcggcagcgg cggcgagttc tttcgcagcg gcgagatcat ccttgattat gccgagcgca
     2341 cgccgaatgt cgaggagatc gtggcgctga aggggctgct ggccgaacgt ggcatccgcc
     2401 tgcgcacggt cgtcgccgga cggcaggcca gtcgcgatct gctccgttcc tggggctatc
     2461 acccgttgcg cctggtggat gccggcgacg aagtgcgcga ggtcgcggtc ggggccgccg
     2521 atgaacgcac ggcactctac gtccgccgca ccctgcgctc gggggcttcg atcggggcag
     2581 acggcgacat cgtcgtgctg ggcgacgtca atgccggagc cgaggtgcag gcaggcggcg
     2641 acgtgctggt ctggggtgcg attcgcggca cggtccacgc cggggtcaac ggcgact
//
LOCUS       DASUBI010000713         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_822951, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000713 DASUBI010000000
VERSION     DASUBI010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822951"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFU72_13890"
     CDS             complement(<1..369)
                     /locus_tag="VFU72_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424631.1"
                     /translation="MAWARRVSRRVFLTASAGVGTVLVAGGAYTLLGREGGVASSPTP
                     TPTATRPALPGGTPAPTALPLARGPADGYLALAPKRLRSGQRERISLALFRGDDPVAG
                     EVALALLQDGQPVAGASAQVA"
     gene            511..2133
                     /locus_tag="VFU72_13895"
     CDS             511..2133
                     /locus_tag="VFU72_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5424632.1"
                     /translation="MLTVSDLSRRYGEYPILERVSFVVNPRDRIGLVGPNGAGKSTLL
                     AILAGELEADAGAVTMTPGARLGHLPQGFADLPGGTLAGLLDVPTGGLLAARDAVERS
                     TAALAEGGEDDLDAALAAYDAALAAFEALGGFAAVDELRAFLAALGLEDVPFETPLGA
                     LSGGQKTRAGLAALLASRPDILLLDEPTNHLDLDALAWLRDFIKGYRGAVLIVSHDRA
                     FLDETVTTIFELSDTTHQLTVYPGNYSAYLAAKLAGEEAQAEAYARQQATITRIEQDI
                     RSVASHAMKTERATQNDFLRRRAKKVARTAKVRERKLERLLESEELVDKPALRWGLSL
                     DFGAPPESGRDVALVEGAAVELGGRTILHDIDLHVGYGERIALTGPNGSGKSTLLKLL
                     LGELAPSAGRVRLGAGVVPGRYAQEQETVALERTVMEQVRARAPLSETEARTFLHLFL
                     FGQETVFKRGAELSYGERARLALALLVLGGANFLLLDEPLNHLDLQSREEFEQALTRF
                     GGTLLIVLHDRYAIERLATRVLELRDGRLAAR"
     gene            complement(2163..2378)
                     /locus_tag="VFU72_13900"
     CDS             complement(2163..2378)
                     /locus_tag="VFU72_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424633.1"
                     /translation="MNEERTPDQENKLDIEREEREIDPERPPAPDEFGVGTEPCPRCG
                     GTGQIDGQRCAHCEGTGYVTVTITPPY"
BASE COUNT          340 a          916 c          966 g          351 t
ORIGIN      
        1 ggcgacctgg gccgaggcgc cggccaccgg ctggccgtcc tgcagcagcg ccagcgcgac
       61 ctcgccggcg accgggtcgt cgccgcgaaa gagggccagc gagatgcgct cgcgctgccc
      121 cgagcgcagc cgtttgggcg ccagcgccag gtagccgtcg gccgggccgc gcgccagcgg
      181 cagcgcggtc ggcgcgggcg tgccgccggg gagggccggg cgagtggcgg tgggcgttgg
      241 agtcggggaa gaggcgactc ccccctcccg gcccagcaac gtgtaggcgc cgcccgccac
      301 cagcaccgtg cccacccccg ccgaggccgt caggaagacc cgccgtgata cgcgtcgcgc
      361 ccatgccatc gccgtcctcc tcgtgccgca cccgccactc gtcattcatc tgtcttgcag
      421 acgacggtag cgcgcgaaga gttcccaacg gcgcgggtgc tacacttaag gttcggcgcg
      481 gcgcccggcc gcgatgactg aggagcccac gtgctgaccg tcagcgatct gagcaggcga
      541 tacggggaat acccgatcct ggagcgcgtc tccttcgtgg tcaacccgcg cgaccgcatc
      601 ggcctggtcg ggccgaacgg cgcgggcaag tcgacgctgc tcgccattct ggcgggcgag
      661 ctggaggcgg acgccggcgc ggtcaccatg acgccgggcg cgcggctggg gcatctgccg
      721 caggggttcg ccgatctccc cggcggcacg ctggcggggc tgctcgacgt gcccaccggc
      781 gggctgctgg cggcgcgcga cgcggtcgag cgcagcaccg cggcgctggc cgagggcggt
      841 gaagatgacc tcgacgcggc cctggccgcc tacgacgccg cgctggcggc cttcgaggcg
      901 ctgggcggct tcgcggccgt cgacgagcta cgcgccttcc tggccgcgct cgggctggag
      961 gacgtgccct tcgagacgcc gctcggggcg ctctcgggcg ggcagaagac gcgcgccggg
     1021 ctggccgcgc tgctggccag ccgccccgat atccttttgc tcgacgagcc gaccaaccac
     1081 ctcgacctcg acgcgctcgc ttggctgcgc gacttcatca agggctaccg cggcgcggtg
     1141 ctgatcgtct cccacgaccg cgccttcctc gacgagacgg tgacgaccat cttcgaactg
     1201 agcgacacca cgcaccagct caccgtctac cccggcaact acagcgccta cctggccgct
     1261 aagctggccg gcgaggaggc ccaggccgag gcctacgccc gccagcaggc gacgatcacg
     1321 cgcatcgagc aggacatccg cagcgtggcc tcgcacgcca tgaagaccga gcgggccacg
     1381 cagaacgact tcctgcggcg gcgggccaag aaggtggcgc gcacggccaa agtgcgcgag
     1441 cgcaagctgg agcggctgct ggagtccgag gagctggtcg acaagccggc gctgcgctgg
     1501 gggctctcgc tcgacttcgg cgcgccgccg gagtcggggc gcgacgtggc gctggtggag
     1561 ggcgcggcgg tcgagctggg cggacggacc atcctgcacg acatcgacct gcacgtcggc
     1621 tacggcgagc gcatcgcgct gaccggcccc aacggcagcg gcaagtcgac cctgctcaag
     1681 ctcctgctgg gcgagttggc gccgagcgcg gggcgggtgc gcctgggggc gggcgtggtg
     1741 cccgggcgct acgcgcagga gcaggagacg gtggcgctgg agcggacggt gatggagcag
     1801 gtgcgcgccc gcgccccgct ctcggagacc gaggcgcgca ccttcctgca tctctttctc
     1861 ttcgggcagg agacggtctt caagcgcggc gcggagctgt cgtacggcga gcgcgcccgg
     1921 ctggcgctgg ccttgctggt gctcggcggg gccaacttcc tactgctgga cgagccgctg
     1981 aaccacctgg acctgcagtc gcgcgaggag ttcgagcagg cgctgacgcg cttcggcggc
     2041 acgctgctga tcgtgctgca cgaccgctac gccatcgagc gcctggccac gcgcgtgctg
     2101 gagctgcgcg acgggcggct ggcggcgcgg tagggccctc accctcggtc ggagataaga
     2161 gcctaatacg gcggcgtgat cgtcacggtg acgtagccgg tgccctcgca gtgggcgcag
     2221 cgctggccgt cgatctggcc ggtgccgccg cagcgcgggc agggctcggt gccgacgccg
     2281 aactcgtccg gggcgggcgg ccgctccgga tcgatctcgc gctcttcacg ctcgatgtcg
     2341 agcttgttct cctgatcggg cgtccgctcc tcgttcatcg ctcgctcctt cgcgttcgcc
     2401 tgggggagcg ggcgggttgc acacccgccc ctaccaatcg atcgtgctcc cctcgcccag
     2461 gagtgggcga ggggcagggg tgagggctag ccctgcgcct gctgccaggc acgggtcagg
     2521 gcacggcggg tgtagacctg ggtcagatgg cgccggtagt cctccccggc gtg
//
LOCUS       DASUBI010000714         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_114925, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000714 DASUBI010000000
VERSION     DASUBI010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114925"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2133)
                     /locus_tag="VFU72_13905"
     CDS             complement(168..>2133)
                     /locus_tag="VFU72_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU5424634.1"
                     /translation="LDSYACTNQGPVEPERKDEVLSPDKQWAALLRDGNLYVRDTAGG
                     AERALTGDAEPFYDYATLPESRLSAVTDRATGKPLPPVVAWSPDSSRLFTYRLDQRQV
                     EPIVLVQSVPPAGSHRPVAHPYRYPLPGDAALATGELLILDLQGNRVPLQTAPIHAGG
                     RGIFETGYAWWGADGQHVYFVRAERGGQGVTLEAADASSGETRTLLEERGASTVFPQH
                     VGMEITNVKVLDGDAAFTWFSAKSGWGHLYLHDAGSGEERNPITFGAWTVRDIVRVDE
                     TDRRIYFTAGGREPGRDPYYRHLYRARLDGSQLELLTPEDAEHSVSFSPSGDYFVDTF
                     SRIDQPPVTVLRAADGRLVAQLEEADVSALLARGWRFPERFSVKARDGVTDIYGSIIR
                     PTTYDSSHRYPMLDAIYPGPQTIRTPKAFPAANTQFWQDQALAELGFIVVTIDGMGTP
                     YRSKQFHDVAYGAAFGEAGGLTDHVVGIKQLAARDASMDLDRVGIYGHSGGGYASTRA
                     LLLFPEFYRAAVSSAGNHDQLGYNASWGERWIGPYDAERYATQDNITLADRLEGKLLL
                     VHGELDDNVHPSLTLRLVDALIAAGKDFEMLIIPNTNHGFFDTRRGLEAQEKHLSQGH
                     PYFVRKRWDHFVRSLLGAEPPAGYRITPPG"
BASE COUNT          307 a          704 c          794 g          328 t
ORIGIN      
        1 ccctcacccc catcccctct cccgtgcgcg ggagaggggc gctgcccatc gagcaggctt
       61 ggccaaaggg aagcatccct aatgccaatc cttgttgtac gccatctccc ctctcccgcg
      121 cacgggagag ggcagggtga gggctccccg ttggggggtg agggaactca ccccggcggc
      181 gtgatccggt accccgcggg cggctcggcg ccgagcaagc tgcgcacgaa gtggtcccag
      241 cgcttgcgga cgaagtaggg atgcccctgc gacaggtgct tctcctgcgc ctccaacccc
      301 cggcgtgtgt cgaagaagcc gtggttggta ttgggaatga tcagcatctc gaagtccttg
      361 ccggccgcga tcagcgcgtc gaccagccgc agcgtcagcg aggggtggac gttgtcgtcc
      421 agctccccgt ggaccagcag cagcttcccc tccagccggt cggccagcgt gatgttgtcc
      481 tgcgtggcgt agcgctcggc atcatagggg ccgatccagc gctcgcccca gctggcgttg
      541 tagccgagct ggtcgtggtt gccggccgag gagaccgccg ccctgtagaa ctccgggaag
      601 aggagcagcg cgcgcgtcga ggcgtagccg ccgccggagt ggccgtagat gccgacccgg
      661 tcgagatcca tggacgcatc gcgcgccgcg agctgtttga tgcccaccac gtgatcggtc
      721 aggccgccgg cctcgccgaa ggcggcgccg taggccacgt cgtggaactg cttcgagcgg
      781 tagggcgtgc ccatgccgtc gatcgtgacc acgatgaagc ccagctcggc cagcgcctga
      841 tcctgccaga actgcgtgtt ggccgccggg aaggccttcg gggtgcggat cgtctgcggc
      901 ccggggtaga tggcgtcgag catcgggtag cggtggctcg agtcgtaggt ggtcggccgg
      961 atgatgctgc cgtagatgtc ggtcacgccg tcgcgcgcct tgacgctgaa gcgctcgggg
     1021 aagcgccagc cgcgcgccag cagcgccgag acgtcggcct cctccaactg cgccaccagc
     1081 cgcccgtcgg cggcgcgcag caccgtcacc ggcggctggt cgatgcgcga gaaggtgtcg
     1141 acgaagtagt cgccggaggg tgagaagctg acgctgtgct cggcgtcctc aggcgtcagc
     1201 agttcgagct ggctgccgtc gagcctggcg cgatagaggt ggcgatagta ggggtcgcgg
     1261 cctggctcgc gcccgccggc ggtgaagtag atgcggcggt cggtctcgtc gacgcgcacg
     1321 atgtcgcgca cggtccaggc gccgaatgtg atggggttgc gctcttcacc gctgccggcg
     1381 tcgtggagat agaggtggcc ccagccgctc ttggccgaga accaggtgaa ggccgcgtcg
     1441 ccgtcgagca ccttgacgtt cgtgatctcc atgccgacgt gctgggggaa gacggtgctg
     1501 gcgccgcgct cctccaagag cgtgcgcgtc tcgccgctgc tcgcgtcggc ggcctccagc
     1561 gtgacgccct gcccaccgcg ctcggcgcgc acgaagtaga cgtgctggcc gtccgcgccc
     1621 caccaggcgt agccggtctc gaagatgccg cgcccgccgg cgtggatcgg cgcggtctgg
     1681 agcgggacgc gattgccctg cagatcaaga atcagcagct cgcccgtggc gagcgcggcg
     1741 tcgccgggga gcggatagcg gtaggggtgg gccaccggac ggtgcgagcc cgcgggcggc
     1801 accgactgca ccagcacgat cggctcgacc tggcgctgat ccagccggta ggtgaagagg
     1861 cgagatgagt ccggcgacca ggcgacgacc ggcggtagcg gcttgccggt agcgcggtcg
     1921 gtgaccgccg agaggcgcga ctcgggcagc gtggcgtagt cgtagaaggg ctcggcatcg
     1981 ccggtcagcg cgcgctccgc gccgccggcg gtatcgcgga cgtagaggtt gccgtcgcgc
     2041 aggagggccg cccactgctt atcgggcgag agcacctcgt ccttgcgctc gggctcgacc
     2101 ggcccctgat tggtgcaggc gtagctgtcg agc
//
LOCUS       DASUBI010000715         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_155252, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000715 DASUBI010000000
VERSION     DASUBI010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155252"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1105
                     /locus_tag="VFU72_13910"
     CDS             107..1105
                     /locus_tag="VFU72_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEU5424635.1"
                     /translation="MSKRVVVYGQFTDDLETERGILEPIGAELIDARGLDDGQLREAF
                     RTADAVLFRRGRVTPEHIDQMQQCKIICTYAAGYDHIDVDAASAKGIMVANTPGYGND
                     EVADHALTLLFALTRRLVPQVLDMAAAVERGDIAGAWNHFPYTPIRRIRGQTMGIIGL
                     GRIGKTTAWKAQGLGMKVIAHDPYVPEAVAAERGVPLVPLDELLRDADYVVIHALLTP
                     ETNKMIGARELALMKPSAYLINVARGKIVDHAALVEALKAGQIAGAGLDVVETEPPPV
                     ELLRQLIQFPNVLVTPHNAWYSEEAIEDRERIAAENVREALQGGIPEAVVNRAAFA"
     gene            complement(1302..1979)
                     /locus_tag="VFU72_13915"
     CDS             complement(1302..1979)
                     /locus_tag="VFU72_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU5424636.1"
                     /translation="MALAPALLVTDFAGTTMEDAGAVLGAYRSALNRFEIPFTEEDLA
                     ARRGASKRAVFEELAGRALPADEASSVAAGALAAFEAALRDEYASGAVREVPGAEASL
                     RRLKQAGVKLALTTGFNRELLNLLVGRLAWQELFDVTLAAEDAPLGRPAPFLIYRAMM
                     ALGVGEVATVAVAGDTPLDLQAGNNARAGWVIGVLSGAHGVATLGATPHTHLLPSIAE
                     VPGLFGL"
     gene            complement(1979..>2738)
                     /locus_tag="VFU72_13920"
     CDS             complement(1979..>2738)
                     /locus_tag="VFU72_13920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEU5424637.1"
                     /translation="RTVAEHMRAAGIEPEGLLQAARPPGSLAAYLELHVEQGGRLAAE
                     RIPIAVVEGIVGIRRYRAHFQGFANHAGTTPMEQRDDALVKAAPYVLAVRDVALSHGI
                     VGTVGTLTVFPGASNVIPGQVTLSVELRGMDEVVLDAAQEELVELAATGGGELAPLTR
                     KAAVPSDQRLVELLAGACEAEGLTVLRMSSGAGHDAMSMASIAPEAMLFAPSMGGISH
                     SPDEYTGPEQCVLAVNALLAALLRVDEDFDRGAA"
BASE COUNT          441 a          981 c          925 g          391 t
ORIGIN      
        1 gccagccgcc gcccgtcgac gtgagcatga acgcgggata gcgaaactgg cgctatactc
       61 gccaccagcg catccggcga ccagtggcca gcagagggag tgggcgatga gcaagcgcgt
      121 ggtcgtgtac gggcaattca ccgatgacct cgagaccgag cgcggcattc tggagccgat
      181 cggtgccgag ctgatcgacg cgcgcgggct cgacgatggt caactgcgcg aggcgttccg
      241 caccgccgac gcggtcctct tccggcgcgg gcgcgtcacg cccgagcaca tcgatcagat
      301 gcaacagtgc aagatcatct gcacctacgc cgccggctac gaccacatcg acgtcgacgc
      361 cgccagcgcc aaggggatca tggtcgccaa cacccccggc tacggcaacg acgaggtggc
      421 cgaccacgcc ctgaccctcc tcttcgccct gacgcggagg ctggtgccac aggtgctcga
      481 catggcggcc gcggtcgagc gcggcgacat cgccggcgcc tggaaccact tcccctacac
      541 cccgatccgc cgcattcgtg ggcaaacgat ggggatcatc ggactgggga ggatcggcaa
      601 gacgactgcc tggaaggcgc aggggctggg gatgaaggtc atcgcccacg acccctacgt
      661 gccagaggcg gtcgcggccg agcgcggcgt gccattggtc ccactcgacg agctgctgcg
      721 cgacgcggat tatgtcgtga tccacgcctt gctcacgccc gagaccaaca agatgatcgg
      781 ggcgcgcgag ctggcgctga tgaagccgag cgcctatctg atcaacgtgg cgcgcggcaa
      841 gattgtcgac cacgcggcgc tggtcgaggc gctgaaggcg ggccagatcg ccggcgccgg
      901 gctggacgtg gtggagaccg agccgccgcc ggtcgagctg ctgcgccagt tgatccagtt
      961 ccccaacgtg ctggtcacgc cgcacaacgc ctggtactcg gaggaggcga tcgaggaccg
     1021 cgagcgcatc gccgccgaga acgtgcgcga agcgctccag ggcggcattc ccgaggccgt
     1081 agtcaaccgc gccgcgttcg cgtaggcccg gccctactgt caagcgcaga cggtgcgtgg
     1141 cgcgtgcgct acggatccgt cgactccact cgggatgaca gggttattta gaggcgtacg
     1201 acgacggcag ccttggtcgt acggcatcta tgaaacattg tcatcctgag cgcagtcgaa
     1261 ggatccgtct tgtcttgcgt ctagtgctcc tcgtcaatca cctacaaccc gaacagcccc
     1321 ggcacctccg cgatgctggg caacaggtgt gtgtggggag tggcgccgag cgtggccacc
     1381 ccgtgcgcgc ccgagagcac gccgatgacc cagccggcac gcgcgttgtt gcctgcctgc
     1441 aggtcgagcg gcgtgtcgcc ggccaccgcc acagttgcca cctcgcccac tcccagcgcc
     1501 atcatggccc ggtagatcag gaagggcgcc gggcggccga gcggcgcgtc ctcggcggcc
     1561 agggtgacat cgaacagctc ctgccaggcg agcctaccga ccagcagatt gagcagctcg
     1621 cggttgaaac cggtcgtcag cgccagcttg acacccgcct gcttgaggcg ccgcagcgat
     1681 gcctcggccc ccggcacttc gcgcaccgca ccgctggcgt actcgtcgcg cagcgccgcc
     1741 tcgaaggctg ccagcgcccc ggccgctacc gatgacgctt cgtcggcggg cagcgcgcgg
     1801 cccgccagct cttcgaagac ggcgcgcttg ctggcgccgc gccgcgccgc cagatcttcc
     1861 tcggtaaacg gaatctcaaa gcggttcagc gcgctgcgat aggcgccgag caccgccccg
     1921 gcatcctcca tcgtggtgcc ggcgaagtcg gtgacgagta gggcgggtgc caatgccatc
     1981 acgctgctcc tcgatcgaag tcttcgtcca cgcgcagcag cgcggcgagc agggcattca
     2041 cggccaacac gcactgctcc ggaccagtgt actcgtccgg cgagtgcgag atgccgccca
     2101 tgctcggcgc gaagagcatg gcttcgggcg cgatgctcgc catcgacata gcgtcgtggc
     2161 cggcgccgct gctcatgcgc agcacggtca gcccctcggc ttcgcaggcg cccgccagca
     2221 gctcgaccag ccgctggtcc gacggcaccg ccgccttgcg cgtcagcggc gccaattcgc
     2281 cgccgccggt cgccgccagc tcgaccagct cctcctgggc agcgtcgagc accacctcat
     2341 ccatcccgcg cagctccacc gagagcgtca cctggcccgg gatcacgttg gaggcgccgg
     2401 ggaagaccgt gagcgtgccg accgtgccga cgatgccgtg gctgagcgcg acgtcgcgca
     2461 ccgccagcac gtagggcgcg gccttgacca gcgcgtcgtc gcgctgctcc atcggcgtcg
     2521 tgccggcgtg gttggcgaaa ccctggaagt gggcgcggta gcgccggatg ccgacgatcc
     2581 cctcgaccac ggcgatcggg atgcgctcgg ccgccagccg gcccccctgc tcgacgtgca
     2641 gttcgagata ggctgccagg ctccccggcg ggcgcgccgc ctggagcagc ccttcgggct
     2701 cgatgcccgc cgcgcgcatg tgctcggcca cggtgcgc
//
LOCUS       DASUBI010000716         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_839267, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000716 DASUBI010000000
VERSION     DASUBI010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839267"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFU72_13925"
     CDS             <1..551
                     /locus_tag="VFU72_13925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424638.1"
                     /translation="RYGVSPGAIGPVISISSALTALGSLYAVRLAARFGDVMVITGSR
                     LAGVVLTLALPFSPVYLLAAALYGARMISSQMAMPVRQSYTMGIIEPGARGSAAGISG
                     VSRRLPASVSPTISGYLFNAGELELPFVAAAACLLLNAGLYYWWFRDVAPDADLVAER
                     ARPAHAGPVMPAGARPEFPRDD"
     gene            complement(778..1743)
                     /locus_tag="VFU72_13930"
     CDS             complement(778..1743)
                     /locus_tag="VFU72_13930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424639.1"
                     /translation="MKFGLTLPNRGVLFGATTAAQMLQTAEIADQSGAFQSVWVGDSL
                     FGKPRMESIALLAGVAARTQHVRLGPACMASFTLRDPVMLAYQWASLDLLAEGRTVLV
                     ACTGIVPQAGYDIEAAVYGVDNKARVERLTEWITILKKLWTEDNVSYHGKHYQFEGVT
                     IEPKPAAKPRPPIWIANNAGGSRELIERTHRRVARHADGWQTSRSDPRDVAWRIEDIR
                     QKARDAGRDPNELETHLYHNINVNEDRDAALKESKEFLDIYYTEDYALDFVESWVATG
                     SPQQCIEHLKVYEAMGFDEVTLRCTSWNQLGQLQRVIEEVLPAFQ"
     gene            complement(1938..3299)
                     /locus_tag="VFU72_13935"
     CDS             complement(1938..3299)
                     /locus_tag="VFU72_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5424640.1"
                     /translation="MVQGRRAALLGLILVLAAVWAAPGQVAAADFTTATIYFSETGHH
                     LSGDFLKTWQANGGLMTFGYPISEPLQQDGTTVQYFERARLEEHPEHAGTPYVVEATL
                     LGNWVAQTRRSEAPFKPLPANTGTGGDSGRRFFPETGHSLAYGFLKYWQENGGLYVFG
                     YPISEEFSEKNPDTGQVYTVQYFERARFEYHPENQGTQFEVLLGRLGAQYATARGVAT
                     SAVKQQPGSVTAFAGLLDPRWSKALRTEDGFAVGIVTADSLNIRSAPRLDAGVVGTTY
                     KRHPVEIQDIVDGDSVNGVTVWYQIGDGQYIAAGWVEPFVPDQPLATYAGHWVDVSLS
                     NFYAVAYDGERPVYAAIITAGRDGKTPTGVYQIFSRVQDETMDSATVGIPKGAPGYYY
                     LEHVMYTQYFKADGYAVHGNWWTDPSNYGGFTSNGCVGLQNSDALWFWNFLSIGSTVQ
                     IHF"
BASE COUNT          485 a         1141 c         1217 g          595 t
ORIGIN      
        1 tccgctacgg cgtctcgccg ggcgcaatcg gcccggtaat cagcattagc tcggcgttga
       61 ccgccctcgg ctcgctctac gcggtgcggc tggcggcgcg cttcggggat gtcatggtga
      121 tcaccggctc gcgcctggcc ggcgtggtgc tgacgctggc gctgcccttc tcgccggtct
      181 acctgctggc ggcggcgctc tacggcgcgc gcatgatcag ctcgcagatg gcgatgccgg
      241 tgcggcagag ctacaccatg gggatcatcg agccgggcgc gcgcggcagc gcggcgggca
      301 tcagcggggt ctcgcggcgc ctgccggcct cggtctcgcc gaccatctcg ggctacctct
      361 tcaacgccgg tgagctggag ttgcccttcg tggcggcggc ggcctgtctg ctgctcaacg
      421 ccgggctcta ctactggtgg ttccgcgacg tcgcacccga cgccgacctg gtggccgagc
      481 gcgcgcggcc ggcgcacgcc gggccggtga tgcccgccgg cgcgcggccc gagttcccgc
      541 gtgacgactg acgggcgctg cccctcgatg acgtcggttg gcgcgtgggg cacggatcct
      601 tcgactgcgc tcaggatgac agggttgata ggggactgac gacgaggatc ggcgacggac
      661 gaagatgcgc ctcgtccgga aggtcatccc ctcccctctg tcatcctgtg ggcagtcgaa
      721 ggatccgacc tgcccgacgt gcccctgtcg tcgtgcgcct ccagccgcag gcgaacccta
      781 ctggaacgcc ggcaggacct cctcgatgac ccgctggagc tggccgagct ggttccaact
      841 ggtgcagcgc agcgtgacct cgtcgaagcc catcgcctcg tacaccttga ggtgctcgat
      901 gcactgctgg ggcgagcccg tggccaccca gctctcgacg aagtccagcg cgtagtcctc
      961 ggtgtagtag atgtccagga actccttgct ctccttcagc gccgcgtcgc ggtcctcgtt
     1021 gacgttgatg ttgtggtaga ggtgcgtctc cagctcgttg ggatcgcgcc ccgcgtcgcg
     1081 cgccttctgg cggatgtcct cgatacgcca ggccacgtcc cgcggatcgg agcgggaggt
     1141 ctgccagccg tcggcgtggc gcgccacgcg ccggtgggtg cgctcgatca gctcgcggct
     1201 gccgccggcg ttgttggcga tccagatcgg cgggcgcggc ttggcggccg gcttgggctc
     1261 gatggtgacg ccctcgaact ggtagtgctt gccgtggtag ctgacgttgt cctcggtcca
     1321 gagcttcttc aggatggtga tccactcggt caggcgctcg acgcgcgcct tgttgtcgac
     1381 cccatagacg gcggcctcga tgtcgtaacc ggcctgcggc acgatgccgg tgcaggccac
     1441 cagcacggtg cgcccctcgg ctagcaggtc gaggctggcc cactggtagg ccagcatcac
     1501 cgggtcgcgc aaggtgaagc tggccatgca ggccgggccg agccgcacgt gctgtgtgcg
     1561 cgcggccacc ccggccagga gcgcgatcga ctccatgcgc ggcttgccga agaggctgtc
     1621 gccgacccag accgactgaa aggcgccgga ttggtcggcg atctcggcgg tctggagcat
     1681 ctgggcggcg gtggtggcac cgaagagcac gccgcggttg ggcagcgtca ggccgaattt
     1741 catggtttac ccctcgtctg gcgtccggct ggcgccgggc ggtcgtcgtc tgtgcgcctg
     1801 atggtgacat cggcggggct ggagggcaag gggcggtgac gagcaacctc gagcgccgac
     1861 gccgggggta caagaagggc gagtttgaaa ctcgcccgca caagaatcgc gtgagatcgg
     1921 gagtgagggc gcgcgcccta aaaatgaatc tgcaccgtcg agccgatgct caggaagttc
     1981 cagaaccaga gcgcgtcgct gttctgcagg ccgacgcagc cattgctggt gaagccgccg
     2041 tagttcgagg ggtcggtcca ccagttgccg tgcaccgcgt agccgtcggc cttgaagtac
     2101 tgcgtgtaca tcacgtgctc gaggtagtag tagcccggcg cgcccttggg gatgccgacg
     2161 gtggccgagt ccatcgtctc gtcctggacc cggctgaaga tctggtagac gcccgtgggg
     2221 gtcttgccgt cgcgccccgc ggtgatgatc gcggcgtaga ccgggcgctc gccgtcgtag
     2281 gccaccgcgt agaagttgct caggctgacg tcgacccagt ggcccgcgta ggtcgcgagc
     2341 ggctggtccg gcacgaaggg ctcgacccag ccggcggcga tgtactgacc gtcgccgatc
     2401 tggtaccaga cggtgacccc gttgacactg tcgccgtcga cgatatcctg aatctccacc
     2461 gggtggcgct tgtaggtcgt gccgaccacg ccggcgtcga ggcgcggcgc cgagcgaatg
     2521 ttgagcgagt cggcggtgac gatccccacg gcgaagccgt cttcggtgcg cagcgccttc
     2581 gaccagcgcg gatcgagcag accagcgaag gccgtcaccg agccgggctg ctgcttgacg
     2641 gcgctggtgg cgacgccccg cgccgtggcg tactgcgcgc ccagccggcc gagcagcacc
     2701 tcgaactggg tgccctggtt ctcggggtga tactcgaagc gggcgcgctc gaagtactgc
     2761 acggtgtaga cctggccggt gtcggggttc ttctcggaga actcctcgga gatcgggtag
     2821 ccgaagacgt agagcccgcc gttctcctgc cagtacttga ggaagccgta ggccaggctg
     2881 tgcccggtct cggggaagaa gcgccggccg gagtcgcccc ccgtgccggt gttggccggc
     2941 agcggcttga agggcgcctc gctacgccgc gtctgggcga cccaattgcc gagcagcgtc
     3001 gcctcgacca cgtagggggt gccggcgtgc tcggggtgct cctccagccg ggcgcgctcg
     3061 aaatactgga cggtcgtgcc gtcctgctgc agcggctcgc tgatcgggta gccgaaggtc
     3121 atcaggccgc cattggcctg ccaggtcttg aggaaatccc cgctgagatg gtgtccggtc
     3181 tcgctgaaat agatcgtcgc cgtcgtgaaa tcggccgccg cgacctgccc cggtgctgcc
     3241 cagacagcag ccagcaccag gatgagcccg agcagcgccg cccgtctccc ctgcaccacg
     3301 tccgctcaat ccctcccgtt cccgccgcgt gtcgtccagt caaggttata acgtgcgagc
     3361 aagccgtttg gcctgcccaa gcggacagga taggaccgcc tctcgccttg ccaactcgtg
     3421 tgcggctgag tacgcttt
//
LOCUS       DASUBI010000717         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1168814, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000717 DASUBI010000000
VERSION     DASUBI010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168814"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFU72_13940"
     CDS             complement(<1..285)
                     /locus_tag="VFU72_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424641.1"
                     /translation="MQRNPHRRPQRSSYRRVTASPTPGAAGEEPGSGLSARLGRFRRR
                     RVYAFAGVSVLLLAILGIIVVPALMRARTAYSQIFVTPMPRVQIAPNASGT"
     gene            complement(370..822)
                     /locus_tag="VFU72_13945"
     CDS             complement(370..822)
                     /locus_tag="VFU72_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5424642.1"
                     /translation="MSAASIGRNRQIISLALALAGIGVAGYLTYVHYNLTALVCTVGN
                     CEKVQTSSYAVIGGVPVALLGLLMYVAIVLLGVARWLRPEQTLTITAAMFAIVLAGTV
                     YAAYLTYLELAVINAICQWCVASALLTVALLINEGYSTFQLVGAPVTN"
     gene            complement(819..1256)
                     /locus_tag="VFU72_13950"
                     /pseudo
     CDS             complement(819..1256)
                     /locus_tag="VFU72_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(1269..1562)
                     /locus_tag="VFU72_13955"
     CDS             complement(1269..1562)
                     /locus_tag="VFU72_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424643.1"
                     /translation="MSNKTPRSDERRSRRAERKAAEERAARNRRLGLVIGAIGIALIA
                     GLALILLPKVTSGNGGVSDITVAEGAPTGVPTSDRTMGNPNAPVTVVEWGDYQ"
     gene            complement(1779..>2431)
                     /locus_tag="VFU72_13960"
     CDS             complement(1779..>2431)
                     /locus_tag="VFU72_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5424644.1"
                     /translation="GSTLCEPIFENVAVPAGAMLGQLNQGWDVVNATLEHSRVWIAAQ
                     AAGIARAALDEAEQYAAQREQFGRLLQDIPAVANHLAVMRRQVELAEYLVDKAARQEE
                     AGDPLFFVWASLAKLIASETALWAAGDAMLLHGGTGYVNEMPISRIFRDSAVLRIYEG
                     AAHIQVKIIERYLERAKIAALFPPTAGLMLEPSELAPLPELIAEIETWQPSQLAAV"
BASE COUNT          374 a          791 c          865 g          401 t
ORIGIN      
        1 cgtgccggag gcgttgggcg cgatctgcac gcgcggcatc ggggtcacga agatctgact
       61 gtaggcggtg cgcgcgcgca tgagcgccgg aacgacgatg atgccgagga tggccaggag
      121 gagcaccgac acaccggcga aggcgtagac gcgccgccgg cgaaagcgcc ccaggcgggc
      181 cgacagcccg ctgccgggtt cttcgcccgc ggcgccgggc gtaggtgatg ccgtcacccg
      241 ccggtagctg gaccgctggg ggcgccggtg cgggttgcgc tgcatcttcg gtcgtgctct
      301 ctgctaacag ccacaatgcc ggggcaatta tccgcagccc ttgtgccggt gcaagagtcg
      361 cacgacgcgt cagttggtta ccggagcgcc aaccagttgg aaggtgctgt atccctcgtt
      421 gatcaagagg gccaccgtca acagcgccga ggcgacgcac cactggcaga tggcgttgat
      481 gaccgccagc tccaggtagg tgaggtaggc ggcgtagacc gtgccggcca gcacgatggc
      541 gaacatggcg gcggtgatag tcagggtctg ctcggggcgc agccagcgcg ccaccccgag
      601 cagcacgatc gccacgtaca tcagcaggcc gagcagggcg actggcacgc cgccgatgac
      661 cgcgtaggag ctcgtctgca ccttctcgca gttgccgacg gtgcagacga gcgcggtcag
      721 gttgtagtgg acgtaggtca ggtagccggc gacgccgatg ccggccagtg ccagcgccag
      781 cgagatgatc tgccggttcc gcccgatgct cgcggcactc acgacttctt cagctccgct
      841 tcgatggctg cccgcacgct gtcccaggtg gccgactgga gctgctgacc attgacgaag
      901 atggtgggcg ttcccggcag cttctgctgc tgggcggagt cgagatcctg ctgcacctgc
      961 ttggccgtgg cgccgctgtc gagacactgg ttgaaatccc cggtattgag gcccagcttg
     1021 ctggcgatgg tcttcaggta gcccttggtc accttgcccg agttctcctt gggctgattg
     1081 gcatagagcg tgtcgtggta ctcccagaac ttgccctggt tggcggcgca atcggcgccc
     1141 tgggccgcga gcttcgactc ggcgccgatg aaggcgtagt cccggaactc gaacttgacc
     1201 ttgccggact gcacgtattg actgacgatc tgcggcttca ccgaactggt gaattgctta
     1261 cagacgggtc attggtagtc gccccactcg acgacggtga caggggcgtt ggggttgccc
     1321 atggtgcggt cgctggttgg cacgccggtc ggagccccct cggcgaccgt gatgtcgctg
     1381 acaccgccgt tgccgctggt caccttcggc aagaggatca gcgccaggcc ggcgatcaag
     1441 gcgatgccga tggcgccgat gaccagtccc aggcggcggt tgcgggcggc gcgctcctcc
     1501 gcggccttcc gctcggcgcg tcgcgaccgg cgctcatccg agcgtggggt cttgttgctc
     1561 atcgatgcga ctgccctttc ctggcagaca actcctcacg tggcggaatg gtaccacgcg
     1621 cgcccggccg cgcatggggg gaggattgtg tacaggccct caccaccccg cccctctccc
     1681 aatgctggga gaggggagga tgcaggagga acatccggat gcccctgtag ctacgcccct
     1741 actcccagca ttgggagaag ggggggtgag ggcgcgcgct acaccgccgc gagctgactc
     1801 ggctgccagg tctcgatctc ggcgatcagc tccgggagcg gcgccagctc cgagggctcc
     1861 agcatcaggc cggcggtggg cgggaagagc gcggcgatct tggcgcgctc gaggtagcgc
     1921 tcgatgatct tgacctggat gtgcgccgcg ccctcgtaga tgcgcagcac ggccgagtcg
     1981 cggaagatgc gcgagatcgg catctcgttg acgtagccgg tgccgccgtg cagcagcatg
     2041 gcgtcgccgg cggcccacag cgcggtctcg ctggcgatca gcttggccag cgaagcccag
     2101 acgaagaaga ggggatcgcc ggcctcctcc tggcgcgccg ccttgtccac caggtactcg
     2161 gccagctcca cctgccgccg catcaccgcc aggtgattgg cgaccgccgg gatgtcctgc
     2221 aggagccggc cgaactgctc gcgctgggcg gcgtactgct cggcctcgtc gagcgcggcg
     2281 cgagcgatgc cggcggcctg ggccgcgatc cagacgcgcg agtgctccag cgtggcgttg
     2341 accacgtccc agccctggtt gagctggccg agcatggcgc ccgcgggcac ggcgacgttc
     2401 tcgaagatcg gctcgcagag cgtcgagccg g
//
LOCUS       DASUBI010000718         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_291272, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000718 DASUBI010000000
VERSION     DASUBI010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291272"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1868)
                     /locus_tag="VFU72_13965"
     CDS             complement(75..1868)
                     /locus_tag="VFU72_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424645.1"
                     /translation="MESNDTMRPALGGTKLSRRSVTKLLAGAAGAPLLGTLIAACGGS
                     SSNAPSATSGSGGTSNATTIAGLATPMNTFQNMATPAATPAAASTTTSAATPQAAAGT
                     PKKGGTIHMSIINDPDTLDPVLSSSATSSSIFTYIYDSLVYIGQDHLPHPWLAEKWDI
                     NTDGTQITFTIRQGIQFHDGTPLDGAAVQAYFDRVLDPKLASIRKTGMGPMTGVDLVD
                     PMTVRCNFSQPYAPFFTQLDGNGITSPAAVQKFGDKFGHNPVGTGPFKFKEWPQGQSV
                     SLVRYENYKNYRDEFKNKGAPYVDGLSWKIIAEQATSTAALLSGELDIAGVDLTQAPT
                     IMSNPQFQVYIWKDRNGYIFVEYNMNKAPFSDLAVRKAVAYALDRDSIVKSAWNGFAT
                     VDLLPIPTGVAGYDASLNQFAYPYDPTKAKQALTDGGYAPGSDGIMAKDGKPLSFTMI
                     VYSGYDALKTAGQIVQSNLKAVGMDCKIQVMDFGAELPLLNAGNFDCDLMRWTSSDPT
                     ILSLMFKTPGWTKQMHDKDLDALLTKADTTLDPQKRLDAVHDAIKYLLAQAIVAPICV
                     DWSMTFVHKYVKDYSLTVFGRGRLEDVWLDK"
     gene            complement(1984..>2652)
                     /locus_tag="VFU72_13970"
     CDS             complement(1984..>2652)
                     /locus_tag="VFU72_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5424646.1"
                     /translation="FGSSQFTMQTTQWGGTLELNEPLPGLDFRPAERYVIQSIEELRV
                     IANPLRLRVLDCLILEPLTVKQVGEKLGISSKKLYYHVGELERIGLVRLVHTEVQSGI
                     QLKYYRAVAAYYYLTASMLHSDRVASYSGASGEFLAFLLESSAGALRRSVANGTIDSN
                     PDSFIVSRRRLRLSPAQATALRDILKAQESAVLSHDDPDGELTMSYVFALFPVNVIGH
                     PEGR"
BASE COUNT          406 a          808 c          947 g          491 t
ORIGIN      
        1 ggggcagggg gtgagggctc cccattgcga gggcgcgcgg gccctccgtg ggggtgaggg
       61 cccgcgccgc ccgcctactt atcgagccag acatcctcca gtcggccgcg gccgaagacg
      121 gtcagcgagt agtccttgac gtacttgtgc acgaaggtca tcgaccagtc gacgcagatc
      181 ggcgccacga tcgcctgcgc cagcaggtac ttgatcgcgt cgtggacggc gtcgagccgc
      241 ttttgcgggt cgagcgtggt gtcggccttg gtcaagagcg cgtcgaggtc cttgtcgtgc
      301 atctgcttgg tccagcccgg cgtcttgaac atcaggctca ggatagtcgg gtcggagctg
      361 gtccagcgca tcaggtcgca gtcgaagttg ccggcattga gcagcggcag ctcggcgccg
      421 aagtccatca cctggatctt gcaatccatg ccgaccgcct tgaggttgga ctgcacgatc
      481 tggccggcgg tcttgagcgc gtcgtagccg gagtagacga tcatggtgaa ggagagcggc
      541 ttgccgtcct tggccatgat gccgtcggag ccgggggcgt agccgccgtc ggtcagcgcc
      601 tgcttggcct tggtcgggtc atagggatag gcgaactggt tgaggctcgc gtcgtagccg
      661 gccacgccgg tcgggatcgg cagcaggtcg accgtggcga agccgttcca ggcactcttg
      721 acgatcgagt cgcggtcgag cgcgtaggcg accgccttgc gcaccgccag gtcgctaaac
      781 ggcgccttgt tcatgttgta ctcgacgaag atgtagccgt tgcggtcctt ccagatgtag
      841 acctggaact gggggttgct catgatggtc ggcgcctggg tcagatcgac gcccgcgatg
      901 tccagctcgc cgctgaggag cgcggccgtg ctggtggcct gctcggcgat gatcttccaa
      961 gagagaccgt cgacgtaggg cgcgcccttg ttcttgaact cgtcgcggta gttcttgtag
     1021 ttctcgtagc gcaccagcga gacactctgg ccctgcggcc actccttgaa cttgaagggg
     1081 ccggtgccga ccgggttgtg gccgaacttg tcgccgaact tctgcaccgc cgccggcgag
     1141 gtgatgccgt tgccgtcgag ctgagtgaag aagggcgcgt agggctgcga gaagttgcag
     1201 cgcaccgtca tcgggtcgac cagatcgacc ccggtcatcg ggcccatgcc ggtcttgcgg
     1261 atcgaggcca gcttggggtc gagcacgcgg tcgaaatagg cctggaccgc ggcgccgtcg
     1321 agcggcgtgc cgtcgtggaa ctggatcccc tggcggatgg tgaaggtgat ctgggtgccg
     1381 tcggtgttga tgtcccactt ctcggccagc caggggtgcg gcaggtgatc ctggccgata
     1441 tagaccaggc tgtcatagat gtaggtgaag atgctgctcg aggtggccga cgacgacagc
     1501 accgggtcga gcgtgtccgg gtcgttgatg atcgacatgt ggatggtgcc gccctttttg
     1561 ggcgtgccgg ccgcggcctg gggcgtggcg gccgaggtgg tcgtgcttgc cgcagccggg
     1621 gtggcggcgg gcgtggccat attctggaag gtattcatcg gcgtcgccag gccggcgatg
     1681 gtggtggcgt tcgacgtgcc gccgctgccg ctggtggccg agggcgcgtt cgaactggag
     1741 ccgccgcagg cggcgatcag cgtgccgagc agcggcgcgc cggccgcgcc cgccagcaac
     1801 ttggtgaccg agcgccgcga cagcttggtg ccgccgagcg ccgggcgcat ggtgtcgttg
     1861 ctctccatca gtcctcctcg ttggccggtc tccggccagg ctcctcggtt ggcgggactc
     1921 acgaccggat cgtcggccac ctgtatccgt gatgggcgcc gccccgccca tcgcccgtcg
     1981 atctcaccgc ccttctggat ggccgatcac attcacggga aaaagcgcga agacgtagct
     2041 catggtgagc tcgccgtcgg gatcgtcgtg gctcagaacc gcactctcct gcgccttgag
     2101 aatgtcgcgc agcgcggtcg cctgcgcggg cgagaggcgc agccgccggc ggctgacgat
     2161 gaaggagtcg gggttgctgt cgatggtgcc gttggcgacc gagcgacgca gcgcgccggc
     2221 gctactctcc agcagaaagg ccaggaactc gccgctggcg ccggagtagc tggcgacacg
     2281 atcggagtgc agcatcgagg cggtgaggta gtagtaggcc gcgacggcgc ggtagtattt
     2341 gagttgaatg cccgattgca cctcggtatg caccagccgc accagcccaa tgcgctccag
     2401 ctccccgacg tgatagtaga gtttcttcga gctgatcccg agcttctcgc ccacctgctt
     2461 gaccgtcagc ggctcgagga tcaggcaatc gagcacccgc aggcgcagag gattggcgat
     2521 gacgcgcagc tcctcgatcg actgaatgac gtagcgctcc gcgggccgaa agtcgaggcc
     2581 cggcagcggc tcattcagtt ccaacgtgcc tccccactgc gtcgtctgca tcgtgaattg
     2641 gctcgatcca aa
//
LOCUS       DASUBI010000719         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_657073, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000719 DASUBI010000000
VERSION     DASUBI010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657073"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..754
                     /locus_tag="VFU72_13975"
     CDS             551..754
                     /locus_tag="VFU72_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424647.1"
                     /translation="MDPTETQREPPPRQLPLPLPAPPPARAVAAPPAALAPGQLWPTL
                     LPGDRLRVRAALLRALREVVDEP"
     gene            744..2798
                     /locus_tag="VFU72_13980"
     CDS             744..2798
                     /locus_tag="VFU72_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU5424648.1"
                     /translation="MSRSAPEKVTPRHRERRAYVYIRQSTPKQVRENRAGQENQYALV
                     ERALALGWPRERIHIIDDDLGRSGQDGQRRGFQELVSAVSLGHVGLILAYEASRLARS
                     NADWYTLLDLATVVGALLADTDGVFDPRDYNDRLLLGLRGMLSEAELHLLRLRMDAGR
                     LRQVEAGTYRQHLPTGLVRLPDGRVVKDPDRQVQHAIELVFARFAALGSCQKVMRSFR
                     DDGLLLPRHQTSGLHAGQLLWKRPAEAAIYDILRNPAYAGAFVYGRRTGRPGRRPGQR
                     GQLRHKAMGEWTAIHRDVYPAYLRWEEFVANQARMSDNASAHARRAAGAPRAGAALLP
                     GLVVCGHCGRQMRTVYKPQTRYCCNAISAGFGAPMCLHLDGACIEDAVVAAFFQALAP
                     AELDLLDEVLTAQQADHERVLRQHDEQVTRAEYEARLARRQYLAVDPDNRLVAGELER
                     RWEQALAALAAAREAAELARATAPPTPALDPALRAQLRDVGPRLPELWASGRLSVVHK
                     KALLRSLIRRVILTRPAADRIAIKVVWVSGAYTELAARPPIWRTADLEDYERLVARVR
                     DLSAAGHQDRAIARRLTAEGFHAARSEGVDAEVVGKIRRGLGQPSLTEQCRRAAKIDG
                     QWTVYGLARELGVDRDWLYRRLYRGTLAASRHPTTGHYLIPDDPQLLARLRAAVARNS
                     HR"
BASE COUNT          402 a         1108 c         1021 g          379 t
ORIGIN      
        1 aactcgccgc cgcgctgggg ttatcggcgg cggagacggc cgcctttgtc gcttcttctc
       61 ccagaatcag ggtggctgcc gcgccgcccc caccgtccgc acacaccaat ctccccccgc
      121 cgcgtacgcc gctgatcggc cgggccagcg aagtcgcggc cctgcgcgac ctcatcgccc
      181 gtggcgagac gcggctggtg acgttgaccg gcgtaggcgg ctgcggcaaa acccatgcag
      241 gacgatgccc cgattggctt gagtgttccc ccaacggaag cggtgctcat cactgacccc
      301 acgcacccgc tctatgggtt gaccctcccc tgcctcggca tcaccaccaa gcagcgcctc
      361 ggccctgcgt gcgtcgtgtg gctcgcgccc ggcatcgagc gcctcgtccc ggtcgccgcc
      421 acgagcctgt cggccatcac ccggcccgtc ccgcggtgcc ggcttgcccc ggcgagtgtc
      481 gccgtcttcc tgcgcgtgct agcgtccgcc ttcgacgaga cgccggcgcg agacgggcag
      541 gaggagcacg atggacccga ccgagaccca gcgggagccc ccgccacggc aacttcccct
      601 ccctctgcca gcgccaccgc cggcccgcgc cgtggcggcg ccgccagcgg ccctcgcccc
      661 cgggcagctc tggccgacgc tgctgccggg ggaccggctc cgggtgcggg cagcgctgct
      721 acgggcgctg cgggaggtgg tcgatgagcc gtagcgcgcc ggagaaggtc accccgcgcc
      781 atcgcgagcg ccgggcctac gtctacatcc gccagtccac gccaaagcag gtgcgcgaga
      841 accgggcggg gcaggagaac cagtacgccc tggtcgagcg ggcgctcgcg ctcggctggc
      901 cgcgtgagcg catccacatc atcgacgatg acctggggcg gtcggggcag gatgggcagc
      961 ggcgcgggtt ccaggagctg gtctcggcgg tctcgctggg gcacgtcggg ctcatcctcg
     1021 cctacgaggc cagccgcctg gcgcgcagca acgccgactg gtacaccctg ctcgacctcg
     1081 ccaccgtggt cggcgcgctc ctcgccgaca ccgacggggt cttcgacccg cgcgactaca
     1141 acgaccggct gctactgggc ctgcgcggca tgctcagcga ggccgaactg cacctgctgc
     1201 ggctgcggat ggatgcgggg cggctgcgcc aggtcgaggc gggcacctat cgccagcacc
     1261 tgcccaccgg cctggtccgc ctgcccgacg ggcgcgtcgt caaggacccc gaccggcagg
     1321 tccagcacgc catcgagttg gtcttcgcgc gcttcgccgc cctggggagc tgtcagaaag
     1381 tgatgcgcag cttccgcgac gacggtctgc tcctcccgcg ccaccagacc agcggcctgc
     1441 acgccgggca actcctctgg aagcgccccg ccgaggcggc catctacgac atcctgcgca
     1501 accccgccta cgccggggcc ttcgtctacg gccgccgcac cgggcggcca gggcgccggc
     1561 cgggccaacg ggggcaactg cggcacaagg cgatgggcga gtggaccgcc atccaccgcg
     1621 acgtgtatcc tgcctacctc aggtgggagg agttcgtggc caaccaggcg cggatgagcg
     1681 acaacgccag cgcccacgcc cgccgcgcgg ccggcgcgcc ccgcgccggc gcggcgctct
     1741 tgcccgggct ggtcgtctgc gggcactgcg ggcgccagat gcgcaccgtc tacaagcccc
     1801 agacccgcta ctgctgcaac gccatcagcg ccggcttcgg cgccccaatg tgcctgcacc
     1861 tggatggggc ctgcattgag gacgccgtcg tcgccgcctt cttccaggcg ctggccccag
     1921 cggaactgga cctgctcgac gaggtgctga ccgcgcagca ggccgaccac gagcgtgtgc
     1981 tgcggcagca cgacgagcag gtcacgcgcg ccgagtacga ggcccgcctc gcccggcgcc
     2041 agtacctggc cgtcgatccc gacaaccggt tggtcgcggg cgagttggag cgccgctggg
     2101 agcaggcgct cgcggcgctg gccgcggcgc gcgaggcggc cgagctggcc cgcgcgaccg
     2161 ccccaccgac gccggcgctc gacccggccc tgcgcgccca actgcgcgac gtcggcccgc
     2221 gcctgccgga gctgtgggcg agcgggcggc tgagtgtcgt ccacaagaag gcgctgctgc
     2281 gcagcctcat tcgccgcgtc atcttgaccc gcccagcggc ggaccgcatc gccatcaagg
     2341 tcgtctgggt gagtggggcc tacaccgaat tggcagcgcg cccgccgatc tggcgcaccg
     2401 ccgacctgga ggattacgaa cggctggtgg cacgcgtgcg cgacctgagc gcggccgggc
     2461 accaggaccg cgcaatcgcc cggcggctga cggctgaggg cttccacgct gcgcgcagcg
     2521 agggcgtcga cgccgaggtc gtggggaaga tccggcgcgg cctggggcaa ccgtcgctga
     2581 ccgagcagtg ccggcgcgcg gcgaagatcg acgggcagtg gacggtctat ggcctggcgc
     2641 gggaactggg cgtggaccgc gactggctct atcggcgcct ctatcgcggc accttggcgg
     2701 ccagccggca cccgacgacg ggacactacc tcatccccga cgacccccaa ctcctcgccc
     2761 gcctgcgcgc ggcggtcgcg cgcaacagtc atcgctagga gggtagtctc atggccgcgc
     2821 taagacgcat atcgcgacgg cccttggggt ggcggcctgc cgccaaggct accgggtgcg
     2881 ctttaccacg gcggctgggc tgaccaacga
//
LOCUS       DASUBI010000720         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_169622, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000720 DASUBI010000000
VERSION     DASUBI010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169622"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..756)
                     /locus_tag="VFU72_13985"
     CDS             complement(52..756)
                     /locus_tag="VFU72_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424649.1"
                     /translation="MHFELCRASAAERRQTLPGDPLVPQPMGVITHAITIDAPPRAVW
                     PWLVQMGSGRGGWYAFDHIDNGGAPSARHVIPELQQIQPGDLMPWLPGATDGFLVQGA
                     TPERALILVVPRQPRSMEIAEEPSAGSWNVLATWALALEPTADGRTRLISRSRVADDF
                     LTKAPSRAPRRLLSTERIYDLLARLPHSLMLPAAGAGHYLMESRMLRGVKRRAEQAWR
                     AAQPEAALIEREGAYP"
     gene            complement(1083..3476)
                     /locus_tag="VFU72_13990"
     CDS             complement(1083..3476)
                     /locus_tag="VFU72_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5424650.1"
                     /translation="MATEAIGEQRDDPQRSPTRRGVFRTDRSSRAGHDLRTAGPSLLI
                     LLALVLVPLLALAPFIPPQVAPATAPAAAFSAERALPHLQVIARQPHPVGSPADAQVR
                     DYLLQQLIALGFTPEIQQADVLSGQFGAMAHVENILVRLPGADSTRAVLITAHYDSVV
                     SGPGTGDDGVSVAAMLETLRALRAGPALRNDLIFLFNDGEEPGLLGTLAFVQNHPWAK
                     DVGVAFDFDADSPATARTTLLWTTKDDGWLVREIGRSAAGMVASSVSTVDKRVDAGND
                     LRMFAAAGITGAHLNVVAGSTRYHNPNDSLANLDQRRLQDYGNAMLALARHFGDLPIA
                     DTRAANAVYFTVFGTTILHYPVGWALPLELLAALGLVALVAFGLWRRRLTVRGLLIGL
                     LLVTLGLLVLGGLAQLGWLALRSAHPESGVFAEHDFYGRSLFMSSLYAGTAAVALAVV
                     PWLNRRFSASHLVVASAAWLVAVVLLAGLDPSQSYFGLLPLLAGVLVLGVLLLVPGGQ
                     GSGWSSARLVTLLLGVVVILGLATSLFYRAAVDGLEAGPLLPIALLTLFLGLLAPQLA
                     LVGQIGRRWLPALGGIVAVGLLAVMLVTSGQSAANPRPNSLVYVLNADSGQALWVSAD
                     ASPDAWTRQVLSASPRHQTLDNLYPGAGADPILTNSAPAASIAGPDLRLLAEERSGET
                     RTLRLRLRSPRQAYRAYLLPGPGVELLAAGIGDKPLVALDQQSLRYDGLPAAGADLTL
                     RVRANGPVTFTVIDESSGLPELPGVTLSARPARFIGAPGPEWVQGDPTLVWRAVEFQ"
     gene            4605..5486
                     /locus_tag="VFU72_13995"
     CDS             4605..5486
                     /locus_tag="VFU72_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424651.1"
                     /translation="MRVGIYIPNGAGADFRGWDVRRAWERTLELARLGERLGFESLWV
                     PDHLQNVRQHDDVPTFEIFPHMTAMALVTERVTIGAAVLCAAFRNAALTVKMMTTLDV
                     ASGGRAEFAIGAGWNEWEFRGYGYGWPSARERLELLEEALEITTRMLQPGRATWHGRH
                     MHIEDVVCEPKGLQEPRMPIIVGGNGPRVTWRLAARFADELNLDGPTIDQIAAWMPTI
                     RQRCEEIGRDPASLPVSSLIRFRGATGQERIEGLQRLAEIGLTRLQVDQVALMDSDEP
                     LYAFVEDCRAAGVELAS"
     gene            5593..6579
                     /locus_tag="VFU72_14000"
     CDS             5593..6579
                     /locus_tag="VFU72_14000"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5424652.1"
                     /translation="MLSTPLCALLGIDHPVLNAPMSGTATAELAAAVSLAGGFGMLGG
                     SSAGGADWLRAQIRAVRSKTDRPFGVGFISSFPGVDDLVRVALEERVAAINHSFADPT
                     PYVAPAHARGVRVFCQVQTVAQAIRAARSGVDVVIAQGTEAGGHTGQAGTLALLPAVV
                     DAVGDIPVVAAGGIADGRGLAAVLLLGASGGWLGTRFVASAEWGGAAQDQQAVLAAGA
                     DDTVRTTVYDQVRGAPFPEGIADRVLRNAFTSAWEGRTGDIAARQAELRRELEEAAER
                     GDPTVVDISAGVAVGLISSLEPAGAIVRRIVEETERVLRERVALLLREAPHR"
BASE COUNT         1015 a         2253 c         2399 g          931 t
ORIGIN      
        1 gataccaggg gcgcacgagc gcgagggtca cgatgagcgg gggcggggcc atcatggata
       61 ggccccttct cgctcgatca gcgccgcttc aggttgcgcc gcgcgccacg cctgctccgc
      121 ccggcgctta actccccgca acatgcgcga ctccatcagg tagtggccag ccccggcggc
      181 cggcagcatg agtgagtgcg ggagcctggc cagcaggtcg tagatccgtt ccgtggagag
      241 cagcctgcgc ggcgcgcgcg acggcgcctt ggtgagaaag tcatcggcga cgcgactccg
      301 gctaatgagc cgggtgcggc catcggcggt cggctccaag gccagagccc aggtcgcgag
      361 tacgttccaa ctgccggcgc tcggctcctc agcaatctcc attgaacgag gttggcgcgg
      421 cacgacgagg atcaaggcgc gctccggcgt cgctccctgc acgaggaagc catccgtggc
      481 gccgggcagc cacggcatga ggtcgcccgg ctggatctgc tggagctccg ggataacgtg
      541 gcgggcgctg ggcgcgccgc cgttgtcgat gtggtcgaaa gcgtaccaac cgccccgccc
      601 agaacccatc tgcacaagcc agggccagac ggctcgcggc ggcgcatcga tcgtaatcgc
      661 gtgagtgatg acgcccatcg gctggggcac gagagggtca ccggggagcg tctggcggcg
      721 ctcagccgcg ctggcgcggc acagctcaaa gtgcatcgga acctccctgc ggcgagcgaa
      781 agtgactgat acgggtagcg gtgccagctc gcacaacggc accacggcca tttgccatca
      841 gcgtggacct ggtgacattg caggggcgtt aaacacgtct gcctggatgt gacaatcgcg
      901 ccaatcgccg gttaatgatt tggagcagga gctatcaggc tcgtgcaaat acacacaggg
      961 ggccgtcggg aggaccttcc tcccaggctg ggccacgcga ttcaccgcca tgagtcgctc
     1021 catttcatag ccaatctcct tcgtcgccag gcggcctgcc cggcctgaac gggtcactcc
     1081 tgctactgaa actcgaccgc gcgccacacg agcgtggggt cgccttgcac ccactccggc
     1141 cctggcgccc caatgaagcg agcgggccgt gcggagaggg tcaccccggg cagctctggc
     1201 aggccgctcg actcgtcgat caccgtgaac gtgactggcc catttgcgcg cacgcgaagc
     1261 gtcaggtcgg cgcccgccgc cgggagcccg tcgtagcgca gtgactgttg gtcgagcgcg
     1321 accaggggtt tgtcgccgat gccggccgcg aggagctcga cgccaggccc cggcaggaga
     1381 taggcgcgat acgcctggcg cggcgagcgc aaccgaagcc ggagggtgcg cgtctcgccg
     1441 ctgcgttcct ccgccaggag cctgagatcg ggtccggcaa tcgacgcggc cggcgcgctg
     1501 ttcgtgagaa tggggtccgc cccggcgccg gggtagaggt tgtcgagcgt ctggtgccgc
     1561 ggcgatgccg acaggacctg tctggtccag gcgtcgggcg acgcatcagc gctcacccag
     1621 agagcctgcc cgctatcggc gttcagcacg tagaccaggg aattcggccg tggattcgcg
     1681 gcgctctggc cgctcgtcac gagcatcacc gcgaggagtc cgacggccac gatgccgccc
     1741 agcgccggaa gccagcgccg gccgatttgc cccaccaggg caagctgcgg ggccagcagg
     1801 cccagaaaca acgtgagcag ggcgatcggg aggagcgggc cagcctcaag cccatcgacc
     1861 gccgcgcgat agaagagcga tgtggccagc cccaggatca ccaccacccc gagcagcagg
     1921 gtcacgagcc gcgcgctcga ccacccgctg ccctgcccgc cggggaccaa caggagcaca
     1981 ccgagcacca ggacgccggc cagcagcggc agcaggccga agtagctctg cgacggatcc
     2041 agccccgcca ggagcacgac ggccaccagc caggcagccg aggcgaccac gagatgggac
     2101 gcgctgaagc ggcggttgag ccacggcacg acggccagtg ccaccgccgc ggtgccggcg
     2161 tagaggctgc tcatgaacag gcttcgtccg tagaagtcat gctcggcgaa cacgcccgat
     2221 tccggatgag ccgatcggag agccagccaa ccaagctgcg cgaggccgcc cagcacgagc
     2281 aggccgagcg tgaccagcag caggccgatc agcaggcccc gaacggtcaa tcgacggcgc
     2341 cacaacccaa atgccacgag cgcgaccagg cccagcgccg ccaacagctc gagcggcagc
     2401 gcccaaccga ccggataatg gagaatcgtg gtgccgaaga cggtgaagta gacggcgttc
     2461 gcggctctgg tatcagcgat cggcagatcg ccaaagtgcc gcgcgagcgc cagcatggcg
     2521 ttcccgtaat cctgcaaccg gcgttggtcg aggttggcca ggctgtcgtt ggggttgtga
     2581 tatctagtgc tccccgcgac gacattgaga tgcgcgcccg tgatgccggc tgccgcgaac
     2641 atgcggaggt cgttgccggc gtcgacgcgc ttgtcgacgg tgctgaccga gctggccacc
     2701 atgccggccg cggaacgccc gatctcccgg acgagccagc cgtcatcctt cgtcgtccac
     2761 aggagggtgg tccgtgcggt agccgggctg tcggcgtcga agtcgaacgc cacgccgaca
     2821 tctttcgccc aggggtggtt ctggacaaat gccagcgtac cgagcaggcc cggctcttcg
     2881 ccgtcgttga agaggaagat caggtcgttg cggagggcag gcccggctcg gagcgcgcgc
     2941 agcgtctcga gcatcgccgc cacgcttacg ccatcgtcgc ccgtgcccgg tccgctgacc
     3001 accgaatcgt agtgcgcggt gatcagcacg gcccgcgtgg aatcggcgcc gggcaggcgc
     3061 acgaggatgt tctcgacgtg ggccatcgca ccgaattgtc cgctgagcac gtccgcctgc
     3121 tgaatctcag gagtgaaacc cagcgcgatc agctgctgta gcaggtagtc gcgcacctgg
     3181 gcgtctgccg gggaaccaac cggatgtggc tgccgcgcga tgacctggag gtggggaagc
     3241 gcgcgctcgg cggagaaggc cgccgccggg gcggtcgcgg gggccacctg cggtgggatg
     3301 aacggcgcca gcgccaggag cggcacgagc acgagcgcca ggagtatcag gagactaggc
     3361 ccggcggtcc ggagatcgtg gccggctcgc gaagaccgat ctgtccggaa gacaccgcgg
     3421 cgggtgggag atcgctgggg atcatctcgt tgctcgccga tcgcctctgt tgccatggga
     3481 gcctcctcca tctcgcggga ccgagggctg tggcggatcg cacgtgtcag acgcagcgct
     3541 cggggccagg cttcgctgcc ggcgcgcgcc tgccggacag cgctggagta gtcgtcatgc
     3601 tggcgagcgg ggaagtcatc aggcacacct cctacgagcc ggatcgggca atcgtgagcg
     3661 accgtcgatg atccgacccc gttggctgag cccacggtag ccgctgcccc gttacagcga
     3721 cgttagcgga ggatcccaaa acgtgacatt ctcgtggcac ggcacggtga caggccctgc
     3781 ccggcccgat tcggttcggg tgcgtcgcgc tcagcgcggc gcccggcggc agctggacgc
     3841 caaaccgcac gcggggcgag tcccggcagc gggcggctcg tgatgatccg gcgcggggag
     3901 ccgccgcggg caggcagctc ccgccgcagg ctcgggcttc gagggcgctg gcaggagcgc
     3961 cactgtggtg aggttgtcgc gtgcgccgcg ggatcgatgc tgaggcgtgg cggcggacag
     4021 cggagactcc gggtggatcc gagaggaggg cagcgtgccc gcccgccaac acgccgccgc
     4081 ctgggtcaag agtgccgctc aggctccctg gctgccgctg ctgatcctgg tcgcgccggt
     4141 cgtctggtgc gtctatcaat cagataaggc cgagcgcatc gtccccatgc tgcccctgcc
     4201 catcatcgcg ttcgtcgtcg gtctgctgct ccggccgcgt cacgtctggt tctctggctc
     4261 ggcagcgcgg tcatcgaatg gatcaccgtg ctcgcctggg acacgtatgc cgaaccggga
     4321 tgggggggag acgcgcggca gcatcataat cgaggcgttc ttctggatgg ccagcggtgt
     4381 cctggtgccg gtctggtgcg gccggcggct gcgggctgga ttcgcggcgg atcggcgtcc
     4441 gaaccagccg ccggccgctt gcgcccccga gtatcgcggg gcgcgttggt gccgccgcgc
     4501 cggcttgcca gagccggcgc cggccgttat ggtgcgcggt acagccgccg gccaggcgcc
     4561 gcgcgggcag ccgcgcgggc gggcagggga gagggcacag gacgatgcga gtggggatct
     4621 acatcccgaa cggcgccggc gccgacttcc ggggctggga cgtgcgccgc gcctgggaac
     4681 gcacgctgga gctggcgcgg ctgggcgagc ggctcggctt cgagtcgctc tgggtgccgg
     4741 accacctgca gaacgtgcgg cagcacgatg acgtccccac cttcgagatc ttcccgcaca
     4801 tgacggccat ggccctggtc accgagcgcg tgaccatcgg cgcggccgtg ctctgcgccg
     4861 ccttccgcaa cgcggcgctc accgtgaaga tgatgacgac gctcgatgtg gccagcggcg
     4921 gccgcgccga gttcgccatc ggcgcgggct ggaacgagtg ggagttccgc ggctacggct
     4981 acggctggcc gtcggcccgg gagcggctgg agctgctcga ggaggcgctc gagatcacca
     5041 cgcgcatgct ccagccgggc cgcgccacct ggcacggccg gcacatgcac atcgaggacg
     5101 tcgtctgcga gcccaagggg ctccaagagc cgcgcatgcc gatcatcgtc ggcggcaacg
     5161 gtccgcgggt cacctggcgc ctggccgccc gcttcgccga cgagctcaac ctcgacggcc
     5221 cgaccatcga ccagatcgcc gcgtggatgc cgaccattcg ccagcgctgc gaggagatcg
     5281 gccgcgaccc ggccagcttg ccagtctcgt cgctgatccg ctttcgcggc gccaccggcc
     5341 aggagcggat cgaggggcta cagcggctcg ccgagatcgg cctgacccgg ctccaggtcg
     5401 atcaggtcgc gctgatggac tccgacgagc cgctgtatgc cttcgtcgag gactgccgcg
     5461 cggccggcgt cgagctggcg tcctgagcgc acgcggcggc ccgcgctggc cgatggattg
     5521 tcgtccgggt tccagacgcc ctcggcacag cccgtgtgtg cctcggcgcc tgggccggcg
     5581 agggagctga tcatgctgtc caccccgctc tgtgccctcc tgggcatcga ccatccggtg
     5641 ttgaatgccc ccatgagcgg cacggccacc gccgagctgg cggccgccgt ctcgctggcg
     5701 ggcgggttcg gcatgctcgg cggcagcagc gccggcgggg cggactggct ccgtgcccag
     5761 atccgcgccg tgcgctccaa aaccgaccgc cccttcgggg tgggcttcat ctcctcgttc
     5821 ccgggggtcg atgacctggt gcgggtcgcc ctcgaggagc gcgtggcggc catcaaccac
     5881 tccttcgccg atccaacccc ctacgtcgcg ccggcacatg cccgcggcgt gcgcgtgttt
     5941 tgccaggtgc agaccgtggc gcaggcgatc cgcgccgcgc ggtccggggt ggatgtcgtc
     6001 atcgcccagg gcaccgaggc cggcgggcac accggccagg ccggcacgct ggcgctgctg
     6061 ccggcagtgg tagacgcggt cggggacatc cccgtggtgg ccgcgggcgg catcgccgat
     6121 ggccgcggcc tcgccgccgt cttgctcctg ggagcgtcgg gcggctggct ggggacgcgg
     6181 ttcgtggcca gcgcggagtg gggcggcgcg gcccaggatc agcaggcggt gctggccgcg
     6241 ggcgccgacg acacggtgcg gaccaccgtc tatgaccagg tgcgcggcgc gccttttccc
     6301 gaggggatcg ccgaccgggt gctgcgcaac gcgttcacga gcgcctggga gggacgcacg
     6361 ggcgacatcg ccgcacgcca ggcggagcta cgccgcgagt tggaggaggc cgccgagcgg
     6421 ggcgacccaa cggtcgtcga catcagtgcc ggcgtcgccg tcggactgat ctcctcactg
     6481 gagcctgccg gggccatcgt ccggcgcatc gtggaagaga ccgagcgcgt gctgcgtgag
     6541 cgggtggcgc tgctgctccg cgaagcgcca caccgatgac gaccgcgtgc cggtgcgg
//
LOCUS       DASUBI010000721         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_78297, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000721 DASUBI010000000
VERSION     DASUBI010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78297"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1306
                     /locus_tag="VFU72_14005"
     CDS             179..1306
                     /locus_tag="VFU72_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEU5424653.1"
                     /translation="MPTVADNHHSTAGHDGTGQLEAILLVGGQGTRLRPLTIGTPKPL
                     LPTAGVPFLAHQIAKAAQSGVKRIVMATAYRAEMFAEVFGDGARFGLEIVYVHEPEPL
                     GTGGGIRNAAAKLRSGPEDQIVVLNGDVLSGHDLGAQLDLHRKADAAVTLHLVEVPDP
                     ARFGCVPTDFDGRVTAFLEKTANPVTCQINAGCYVFTRRYIDAMPPGVVLSVERDTFP
                     GLIQADETVMGYLESAYWLDVGTPEAYVRGSSDLVLGELASSAVPGECGRALLLPGAT
                     IDPSATIIGGTAVGAGSVVAARAVVDGSVLFDGVTIGEGAKVTASVVGAGAKIGAGAV
                     LDGVVIGTGAEIGPRNELRNGMRVWPGVRLDHTAVRFSTDA"
     gene            1316..2269
                     /locus_tag="VFU72_14010"
     CDS             1316..2269
                     /locus_tag="VFU72_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424654.1"
                     /translation="MTAGEIRPAAATPAERQWVAPFVIDVKSAFGVHRRGYGDPAFAV
                     DGSGAIWRACQTPQGAGTLRVTWRADRDLGTLVTASAWGEGAGWLLASLPAMVGADDR
                     PEDFVAHHDVLRAVARRRAGLRIGKSGLVLEALAPAVLEQKVVGKEATRAWRTLLRRF
                     GEPAPGPAPPGMRAPPAARIWAGIPSWEWHRAGVEAVRARTITGAARVADRLEEIVSL
                     PSAEADRRLRSLPGVGLWTSAEVRQRACGDPDAISVGDYHLPKLVGCALTGQPADDAG
                     MLALLAPYQGHRHRAAMLAAMTGPPPPRHGPRNPIRDYRSF"
     gene            2441..>2820
                     /locus_tag="VFU72_14015"
     CDS             2441..>2820
                     /locus_tag="VFU72_14015"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5424655.1"
                     /translation="MGEYSGRRLSVAELSLSPAGFERGPVNAITDVAGVRVGHTTLVE
                     GANIRTGVTAVVPDGVDRAGGELSAGLFVGNGFGKLIGATQLVELGVIETPIVLTGTL
                     SAFRAADALVSYMLALPGNENRRTL"
BASE COUNT          413 a          967 c         1013 g          427 t
ORIGIN      
        1 acgccgacag catcaagacc acgctccgcg ccctgatcga cgaccagggc cagcgcgagg
       61 cgctcggccg cgcgggctac gccaggtcgc gtgaatttac ctgggccgct tcggcgcaag
      121 cccacatagc ttgctatgag cgcgccgcat cggcggggca ggggtagtct gcctggtcat
      181 gcccaccgtt gcagacaacc accacagcac cgccggccac gacggcacgg gtcagcttga
      241 ggccatcctg ctcgtcggcg ggcagggcac aaggttgcgc ccgctgacta tcgggacacc
      301 gaagccgctg ctgccgaccg ccggcgtgcc gttcctggcc catcagatag cgaaggcggc
      361 ccagtccggc gtcaagcgga tagtgatggc cacggcctac cgcgcggaga tgttcgccga
      421 ggtcttcggc gacggcgccc ggttcggcct cgaaatcgtg tacgtgcacg aaccagagcc
      481 gcttggcacc ggcggtggca tcaggaacgc ggcggccaag ttgcgcagcg gcccggagga
      541 ccagattgta gtgctcaacg gggacgtgct gtccggccac gacctcggcg cccaactcga
      601 cctgcaccgc aaggcagacg ccgcggtcac gctgcacctg gtcgaggtgc ctgacccggc
      661 cagattcggc tgcgtgccta ccgacttcga cggccgggtc accgcgttcc tcgaaaagac
      721 cgcgaacccg gtgacctgcc agatcaatgc cggctgctac gtgttcaccc ggcggtacat
      781 cgacgcgatg ccgccgggcg tcgtgctgtc ggtcgaacga gacacgttcc ccggcctgat
      841 ccaggctgac gaaaccgtca tgggctacct ggagtccgcc tactggctcg atgtcggtac
      901 ccccgaggct tacgtgcgag gttcgagcga cctggtgctt ggtgagcttg cgtcgtcggc
      961 cgtacccggc gaatgcgggc gggccctgct cttgcccggg gcgacgatcg acccgtcggc
     1021 gacgatcatc ggcggtaccg cggtcggggc tggcagcgtt gtcgcggctc gtgccgtcgt
     1081 cgacggctct gtgctgttcg acggcgtgac gatcggcgag ggtgccaagg tgaccgccag
     1141 cgtggtcggc gctggcgcga agatcggcgc cggcgccgtt cttgacggcg tcgtgatcgg
     1201 caccggggcc gagatcggcc ctcgcaacga actgcgcaac ggcatgcggg tctggccagg
     1261 cgtgcggctc gaccacacgg cggtcaggtt ctccaccgac gcctagcggc gcgccatgac
     1321 ggcaggggag atcaggccag ccgccgcgac gccggccgag cggcagtggg tcgcaccttt
     1381 cgtgatcgat gtcaagtcgg cgttcggcgt gcaccgcaga ggctacggcg atcccgcgtt
     1441 cgccgttgac ggctccgggg cgatctggcg agcgtgccag accccgcaag gcgcgggcac
     1501 gctacgggtc acctggcgcg ccgaccgtga cctcggcacg ctggtgaccg cctcggcatg
     1561 gggtgaaggc gccggctggc tgctcgcgtc gctgcccgcc atggtcggtg cggacgaccg
     1621 gcctgaggat ttcgtcgccc accacgacgt gctgcgcgcc gtggctcgca ggcgcgcggg
     1681 gctgcggatc gggaagtctg ggctggtgct cgaggcgctc gcgccggcag tactcgagca
     1741 gaaagtggtc ggcaaggaag ccaccagggc atggcggacg ctgctgcgac gctttggcga
     1801 gcccgcgcct ggaccagcgc cgcccgggat gcgggcaccg ccggcggctc ggatctgggc
     1861 tggcatcccg tcctgggagt ggcacagggc cggagtcgag gcggtgcggg cgagaacgat
     1921 caccggggcg gcgcgggtag ccgacaggct cgaggagatt gtcagcctgc cgtcggctga
     1981 ggccgaccgc aggctgcgat cgctgccggg agtcggcctg tggacatcgg ccgaggtcag
     2041 gcagcgcgcc tgcggcgacc cggacgcgat ctcggtcggc gactaccacc tgcccaagct
     2101 ggtcggctgc gcgctcaccg gccagccggc agacgacgcc ggcatgctcg ccctgctcgc
     2161 cccctaccag ggccaccgcc accgcgccgc gatgctggcc gccatgaccg gcccgccccc
     2221 gcctcgccac ggcccccgca accccatccg cgactaccgc tcgttctgac gggtgcgcag
     2281 gagccgcgac ttcgacttag ccctgattac gacgccttag ccctggttgg cgacgaattt
     2341 tgtcggattg atcctggttc ggtcgtcggt aaccagggct atgtgggcgt aaccagggct
     2401 atgtgggcgt aaccaggcga ccgcggctac cgttggtccg gtgggtgagt actccggacg
     2461 gcgcctctcg gtcgcagagc tgagcctgag tccagcgggt ttcgagcgcg ggccggtcaa
     2521 tgcgatcacc gatgtggccg gcgtccgggt cgggcacacc accctggtcg agggggccaa
     2581 catccgcacc ggggtcaccg cggtcgtgcc ggacggcgtc gatcgagccg gtggcgaact
     2641 ctccgcaggc ttgttcgtcg ggaacgggtt cggcaagctg atcggggcca cccagctggt
     2701 cgagctcggg gtgatcgaga cgccgatcgt gctgaccggc accctgtcgg cttttcgcgc
     2761 cgccgacgcc ctggtcagct acatgctggc cttgcccggc aacgaaaaca ggcggaccct
//
LOCUS       DASUBI010000722        13799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1030101, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000722 DASUBI010000000
VERSION     DASUBI010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030101"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFU72_14020"
     CDS             complement(<1..280)
                     /locus_tag="VFU72_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424656.1"
                     /translation="MRTYERMWRLPSRLGGVCGTDPSTALRRKGKKDEADDNDSRRRP
                     PPINPVIPTGVAGSVLDRRWTRSEGSPLLPRKVRALAQVAHEVFEQAHG"
     gene            377..820
                     /locus_tag="VFU72_14025"
     CDS             377..820
                     /locus_tag="VFU72_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5424657.1"
                     /translation="MKVSTRGEYGMRAMVSLAKMYGHGPMPLAAVATDSSVPAAYLEQ
                     LMGPLRRAGLVETTRGAHGGYALARPPQEILIGQVYRVMEGPIAPMSCVSEDVDDDDT
                     DLCPMIDGCATRVVWLKVRDSIAAALDSTTLADLLGKHSPTATAH"
     gene            892..2040
                     /locus_tag="VFU72_14030"
     CDS             892..2040
                     /locus_tag="VFU72_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU5424658.1"
                     /translation="MYLDHAATTPVDPVVLEAMLPYFSARFGNPSSIYAAGREARAAL
                     DQARAALAAILGCQARELVFTSGGSEADNLAIKGVAWWARQQHRGNHIVTTAFEHHAV
                     LHSVAYLEKFGFEATYVPPDRRGLIQPEAVAAALRPDTALVSVMYANNEIGTIQPIAE
                     IAAIARARGIPVHTDAVQAAGSLPLDVRALGVDLLSLSAHKFYGPKGVGLLYVRRGTP
                     ILWQQSGGAQENNHRAGTENVPAAVGMAAALRLAEDERATRSARILCLRERLIDGLLE
                     QIPGSHLNGDRERRLPNNVNVSFEGVEGETILLNLDMHGIAASSGSACTTGSTEPSHV
                     LTALGLPAELVRSSIRLSLGKETSAEEIDRTSEVLAQSVAHLRRLAAA"
     gene            2196..3464
                     /locus_tag="VFU72_14035"
     CDS             2196..3464
                     /locus_tag="VFU72_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEU5424659.1"
                     /translation="MEQPIVIETREELIYLLSEASQLEHMILCEYLYAAFSLKRGADE
                     GITPEQLEMTKRWERAVSEVAAQEMLHLTLASNLLTAIGASPCFHRPNFPKFAKYFPP
                     GMQFSLLPFGEDALIHFLFLERPEGMERSDAPEYAVLAEANPAAQGSEVVPMVEDFQT
                     VGHLYRGIEQGFRHLVDKYGERQVFVGPPQAQATQDYFNWDELIPVRDLDSAITAIET
                     IVEMGEGARGDWQDSHYGKFLRVYEEYRAAKDADPDFEPARPVVPAVCRLLSDESNLD
                     LIEDPDTARVTDLFNASYELLLQVLVRFFIHEDENPAEFQALADLAVSLMFGPLRTLG
                     RLLTTLPVGPGLPGKTAGPTFEIFGLRSYLLPHRDSAWVLLHERLLELAEDCAEIETE
                     ETKRTLDRIAESLRKGAAKLEPYLEPGASA"
     gene            3677..4549
                     /locus_tag="VFU72_14040"
     CDS             3677..4549
                     /locus_tag="VFU72_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5424660.1"
                     /translation="MTLLALALLVAAAFMHATWNLLAKRVGGGPPFVWLFATCSTLIY
                     APLVLLVLLVQQPELGPSQFLFMGGSAVLHLSYFLTLQQGYRVGDLSLVYPLARGTGP
                     TLATIGAIVILGEHPGPIALAGAALVVVSVFLITGGFAAPRRGSAAARPAIALGLVTG
                     CFIASYSLWDKHAVSALAVPPLVLDWSTSLFRAIVLSPVARRRWCEVRGIWRAHRWET
                     LGVGVLSTLAYLLVLFALSFTPVSRIAPAREMSILVGTFFGTRLLREGRGQVRRRLAA
                     ASLMVAGVIALALG"
     gene            complement(4824..6119)
                     /locus_tag="VFU72_14045"
     CDS             complement(4824..6119)
                     /locus_tag="VFU72_14045"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424661.1"
                     /translation="MNAKQVASEHRDEVEARGTTMSQRLPFLGLLAAGFISALGSNFT
                     AVAIPWFVLVTTGSAARTGITLAFYTLPILITGIVGGALVDRLGFVRSSILSDLGGGL
                     TMALIPLLYHTVGLDFWALLLLVFLRGMLVNPGATARMSLVADLAEQAGTSLERANSL
                     TGGAQRLALLFGPPIAGILIAFMGVSNVLYLDGLSFWFSAAIFSIAIAGRVTRRHQSL
                     ARDSYWRETLAGFHFLRQDRVLLWVVLIVAVASVISEPFYTIILPVYAKEVYGSAVDL
                     GLMFSALGVGALLGLLSYALVGQRLPRELVVVVGFLVRAGTFFVPLLRPELPILLGSI
                     VLNATFFEPLNPLSLVILQEHTPAALRGRVFGTINALSNGTLPLGTLLGGALIGGLGL
                     MGALAVLAGATLAQSLSLPLIPAMRQLEAPARPAKGGVE"
     gene            complement(6186..7001)
                     /locus_tag="VFU72_14050"
     CDS             complement(6186..7001)
                     /locus_tag="VFU72_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424662.1"
                     /translation="MTPSAARRSITTEAGAAFASWQVLLKERWEHFGRQGYLFIWVVR
                     PIFDLALAALIYAGGRTDLIPYLVVAMTANSAVFNTLYWIGEILDRERRKGTLVMLFL
                     APCARLSWLLGFAASGIVETAGAALTVGVSGMLLFGVRFDPNLLTLAVTLALFLASLL
                     GLGLILSAAGLLIRKSNELANLVFPAVMLLGGSYYPVSQLPDALRIPARALPMGYGME
                     ALSAAALHHASLSQVRFDLVPLAGFAVVLPLLGWWSFGRIERLVRRRGELDLY"
     gene            complement(6964..7848)
                     /locus_tag="VFU72_14055"
     CDS             complement(6964..7848)
                     /locus_tag="VFU72_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424663.1"
                     /translation="MQDRVIVAFLRTPEPPQPVPPVYPRPAGQASFADICLASAGCYV
                     RRTISSLIDLVRWPLFPAVLFLTMLLTYRVAGRSTVDGYDVAGYLLVGTFGLALWSSN
                     LWGSGYAIEYERSEGTLTALFLTPASRAAVVLGYGLGSTALYVLPSALVVGLLAWLSG
                     ARLLVTDPLAALVALVALVGASFALGYALAGFFVLTRRANLLANSLQSPIFLLSGMLV
                     PVAALPGPLRPLASVFPISDGMTALRAALLGGAGLGDIGGPLLRLLLMSAVLVLAGTL
                     LLGRVEHDAKRGATLDYD"
     gene            complement(7833..8816)
                     /locus_tag="VFU72_14060"
     CDS             complement(7833..8816)
                     /locus_tag="VFU72_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424664.1"
                     /translation="MLAIEAEGLRRVFRPKRRLLSRGPERVAVDGVDLAVERGTIFGL
                     LGPNGAGKTTTIKMLSTLLIPSGGRARVAGYDVVRQEREVRRRLGVLLGGDRGLYAKI
                     SARDNLRYFAHLYGLWEQPAARVDELLALVGLSDRADERVETYSRGMKQRLHLARALL
                     HDPPVIFLDEPTIGLDPAAAVNLRAIVESLVPAHTVLLTTHYLFEAEELCDRIAIIDQ
                     GRILVEDTPAGLKRRVGGDQRYRFTVRGDDPAVRDALRALPAVRELAGARADDGWVYT
                     LRCAPDAPILDEAMQIIHGRGMRTTAVETLEPTLEDAFLALTEGAEACKTA"
     gene            complement(8803..10095)
                     /locus_tag="VFU72_14065"
     CDS             complement(8803..10095)
                     /locus_tag="VFU72_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5424665.1"
                     /translation="MSVSEQTFPAEGIERVSLELERRSVSVEWVESDVIAVQGEAVAA
                     EPAGDELIVGGAGAPHGVRISVGQIAFVAQGGGSGETVRLRLPRTLPTFRLRVRQGNV
                     RLSDARGQCDLRLDRGDLVMHGGQGQATIAIGSGDLEVEEYQGPLSITGGSGQKELRQ
                     VVGKIALTGGSGDIELLGGRGDLTLTTGSGDVDVSERDCEEVVIKAGSGDITLKHGRA
                     GSLAIVTGAGDVDCDCRLGLHEHSIESGSGDIELSVPRDISARIEATTHRGRIDSEIP
                     LVEVGQRGKRSLFGRRLVGSVGEGAERADISLRSGSGDIALRWLQTPANPQEQPPARP
                     QPPSQPVSPVVPLGPPAAPATPGEPVAAAAAAPETSGPQPEQTEQPGNGGRRRVLEAL
                     AAGSISPAEAARLLDALDRAGLSSPSRSSTGGDDDAGY"
     gene            complement(10092..10493)
                     /locus_tag="VFU72_14070"
     CDS             complement(10092..10493)
                     /locus_tag="VFU72_14070"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEU5424666.1"
                     /translation="MFPAPQQCPTCGHPLELRELSCPACETVVRGRWAAGPFSRLSPE
                     QQTFLRLFVRSRGNLSEVERTLGVSYPTVRAKLEEVIELLGDQEPPAAPPPPAATTRP
                     SARDELLQRIARGEVGVDEGLARLGSLTEES"
     gene            10727..11758
                     /locus_tag="VFU72_14075"
     CDS             10727..11758
                     /locus_tag="VFU72_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5424667.1"
                     /translation="MKRPSTQRQRIGPAVRRLRRQQGLTLDALAMTAEVSPSHLSRLE
                     RSQTLPSFPVLAKIAEALGADVNELVRLEHDVAQLDAELEGYLDMLGIAAEQRAEILH
                     LSIETRRDLTGRLRALAEDRLTPLALQDTIARVLCQPGGLPGLAQTARYICDSGLSGT
                     GYARALLELDQLLGRRRLLAGGASLLPLAPGTALLPHFRHVFGSEPLDPAVATWWLEP
                     HQNAYPRGTPHRPLRAILGRRALESPLGPVMARSLLEVIARPEGEVALTERELGPVNA
                     LAVDGSYALLEDLPGEPETDCCAALWLAGQQRVAPLDHLIDSLWDGLPPHEKYPTAVA
                     EALRRAARA"
     gene            11896..13098
                     /locus_tag="VFU72_14080"
     CDS             11896..13098
                     /locus_tag="VFU72_14080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5424668.1"
                     /translation="MYDTFQPVQPVQQASAIPLSSAEPRRVCLLSDDLSGPPDEGVKK
                     VTLQLAAALAPHHHVSLLSTTPTSQGSAARYVPAPRTFLSRSLRAELRRLQPDVLIYA
                     TRRSATFNSFLRARLLKRYCPTATLVLLALQTRRHPAWQQRLIRHLLPDLAGVQSGAN
                     RDYLTGLGCHVTLVRGGVDSAAFQPVDEARRQLLRARYGLRDDLPVVLHVGHLHRERG
                     VGVLAALASGHECQVVLVASSSTPQEADIGDELRAAGVTLLTTYLPHIEELYQLADCY
                     VFPVLSTDAAIELPLSVLEALACDLPVVTTRFGGLPQAFANAPSEGLVFIDSPQELVT
                     EALRLCREDSGSTRPLALPFAWEAVAADLLAQALAIRGEPNGRAVEIEGLSQGETPWA
                     GEVSHAGQ"
     gene            13085..>13799
                     /locus_tag="VFU72_14085"
     CDS             13085..>13799
                     /locus_tag="VFU72_14085"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5424669.1"
                     /translation="MPGSEGGGASPGTVADAVVVAEAPRRWATNAAPDARGGPEVTLL
                     VRLLDEGERATPAPGLDPARFIDWCRRNKFPLLSLERHTPAWLAEDPDFARALAAESD
                     WFATQHGEYHRLRDAWLARGIPCLLIKSAGEHPAFPHSSDNIDILVHPEQGLAARETL
                     RDLGYVELRNIEEPGKFLFRRFSDGVCVSAIHLHERIAWFVGFLDDQAVWRRARAAHD
                     DPAVTIPASEDAVLINLAHA"
BASE COUNT         2147 a         4974 c         4608 g         2070 t
ORIGIN      
        1 ctccgtgcgc ctgctcgaac acttcgtgcg cgacctgggc cagagcccgg actttacgcg
       61 gaagtagggg gctgccttcc gagcgggtcc agcgccggtc gaggacggat cccgccactc
      121 cggtcgggat gaccgggtta attgggggcg gacgacgacg ggagtcgttg tcgtccgcct
      181 catccttttt ccctttcctc ctgagcgcag tcgaaggatc ggtgccgcac acgccaccca
      241 accgactcgg caggcgccac attctctcgt acgtacgcac tactcctcct ctccgctctc
      301 gcccaacgct tgaccggaat gagactcaat cgtatgattg ggccagccga ttggctcgcg
      361 agaggtgcgc acgcccatga aagtttcaac ccgaggcgag tacggaatgc gcgcgatggt
      421 ctcgctcgca aagatgtatg gtcacggacc gatgccgctc gcggcggtcg ccaccgattc
      481 gagcgttccg gcggcctacc tggagcagct catgggcccg ctgcgccgtg ccggcctggt
      541 tgagaccacg cgcggcgccc acgggggcta tgccctggcg cgcccgcctc aggagatcct
      601 gatcggccag gtctaccggg tcatggaggg gccgatcgcg ccgatgagct gtgtcagcga
      661 ggacgtcgac gacgacgaca ccgatctctg cccgatgatc gacggctgcg ccacgcgagt
      721 tgtctggctc aaggtgcgcg acagcatcgc tgccgcgctc gactcgacga ccctcgccga
      781 ccttctgggg aagcactcgc ccaccgcgac cgcgcattag cgggccgtac ggccggactg
      841 accgcgccaa cgcctgacac atcctggagg ctgtccctga accggacgga aatctacctc
      901 gaccacgcgg ccacgacgcc ggtcgatccc gtcgtgctgg aggccatgct cccctatttc
      961 agcgcgcgct tcggcaaccc ctccagcatc tacgccgcgg ggcgcgaggc gcgcgccgcg
     1021 ctcgaccagg cacgggccgc gctggccgcg attctcggct gtcaggcgcg cgagctcgtc
     1081 ttcaccagcg gcgggagcga ggccgacaac ctggcgatca agggcgttgc ctggtgggcc
     1141 cgccagcagc accgcggcaa ccacatcgtc accaccgcct tcgagcacca cgccgtgctg
     1201 cacagcgtcg cctacctgga gaagttcggc ttcgaggcga cctacgttcc gccggatcgt
     1261 cgcgggctga tccagcccga agcggtcgcc gcggcgcttc gccccgacac ggccctcgtc
     1321 tccgtgatgt acgccaacaa cgagatcggc accatccagc cgatcgccga gatcgccgcg
     1381 atcgcccgcg cgcgcggcat ccccgtgcac accgacgccg tccaggccgc tggcagcctg
     1441 ccgctggacg tgcgcgcgct cggcgtcgat ctcctctcgc tctccgccca caagttctac
     1501 ggcccgaagg gtgtggggct gctctacgtg cggcgcggca cgccgatcct ctggcagcag
     1561 agcggcggcg cgcaggagaa caaccaccgc gccggcaccg agaacgtgcc cgccgcggtc
     1621 ggcatggccg cggcgctgcg actggccgag gacgagcgcg caacgcgcag cgcgcgcatc
     1681 ctctgcctgc gcgagcggct gatcgacggc ctactggaac agatccccgg cagccacctc
     1741 aacggcgacc gcgagcggcg cctgccgaac aacgtcaacg tctccttcga gggggtcgag
     1801 ggtgagacca ttctgctgaa cctcgacatg cacggcatcg ccgcctccag tggctcggcc
     1861 tgcacgaccg gctcgaccga gccgagtcat gtgctgaccg cgcttggcct gccggcggag
     1921 ctggtgcgca gcagcatccg gctgagcctg ggcaaggaga cctcggccga agagatcgac
     1981 cgcaccagcg aggtgctggc tcagagcgtg gcccacctgc gccgcctggc cgccgcctag
     2041 cccaatcgcc tctcaaccat tacaatctca gcaacgtaca ccagcgagcg ttctggcagc
     2101 tgccgcggca gcgcgcacac ggccgggcgg attctccgcc tccggcaatg ggagccgcag
     2161 atcggacgcc cggcgaacgg gaatggggag gcgagatgga gcagccaatc gtcatcgaga
     2221 cccgggagga gttgatctac ctcctctccg aagcctcgca gttggagcac atgattctct
     2281 gcgagtacct ctacgccgcc ttcagtctga agcgcggcgc cgacgagggg attacgcccg
     2341 agcagttgga gatgaccaag cgctgggagc gcgcggtcag tgaggtcgcg gcgcaggaga
     2401 tgctgcacct gacgctggcc agcaacctcc tcactgccat cggcgcctcg ccctgcttcc
     2461 accggccgaa cttccccaag ttcgccaagt acttcccccc cggcatgcag ttctccctgc
     2521 tgccgttcgg tgaggacgcg ctgatccact ttctcttcct ggagcgcccg gaggggatgg
     2581 agcgctccga cgcgccggag tacgccgtgc tggccgaggc caacccggcc gctcagggca
     2641 gcgaggtcgt gccgatggtg gaggacttcc aaacggtggg ccacctctat cgcggcatcg
     2701 agcagggctt ccgccacctg gtcgacaagt acggcgagcg acaggtcttc gtcggtccgc
     2761 cgcaggccca ggcaacccag gattatttca actgggacga gcttattccg gtgcgtgatc
     2821 tcgactcggc tatcaccgcc atcgagacga tcgtggagat gggcgagggc gcgcgcggcg
     2881 actggcagga ctcccactac ggcaagttcc tgcgcgtcta tgaagagtac cgcgcagcca
     2941 aggacgccga ccccgatttc gaaccggccc gtccggtcgt gcccgccgtc tgccgcctgc
     3001 tcagcgacga gagcaacctc gacctgatcg aggatccgga caccgcgcgg gtgaccgacc
     3061 tcttcaacgc cagctacgag ctgctgctgc aggttctggt gcgcttcttc attcacgagg
     3121 acgagaaccc ggccgagttc caggcgctgg ccgatctcgc ggtgagcctc atgttcggtc
     3181 cccttcgcac gctcggccgc ctgctgacca cgctgccggt cggccccgga ctgccgggca
     3241 agaccgccgg gccgaccttc gagatcttcg gcctgcgcag ctacctgctg ccccatcgcg
     3301 actcggcctg ggtgctgttg cacgagcgcc tgctggagct ggccgaggat tgcgccgaga
     3361 tcgagacgga ggagaccaag cgcaccctcg accgcatcgc ggagagcctg cgcaagggcg
     3421 cggcgaagct ggagccttac ctggagccag gcgcgagcgc gtagggagcg ggagaggccg
     3481 ccgaggaccg cggtgccgcg tgaagccggg cagacacagg ggtctgcccc taccgcctac
     3541 gcaacctgct tgtttcctgt aggggcaggc ccctgtgtct gccctcgctc cccacgcacc
     3601 attcttccat gaaagtcgcc ctgcgtgcgg ataccggttc ggcacccgat gcgcgctcgt
     3661 gggggcgccg tcgccaatga ccctgctcgc gctcgcgctc ctggtcgccg ccgccttcat
     3721 gcacgccacc tggaacctgc tggccaagcg cgtcggcggc ggccctccct tcgtctggct
     3781 gttcgccacc tgctcgacac tgatctacgc gccgctggtg ctgctggtgc ttctcgtaca
     3841 gcaaccagag ctcggtccaa gtcagttcct tttcatgggc ggcagcgccg tgctgcacct
     3901 ctcctacttt ctgacgctgc agcagggcta ccgcgtcggc gatctgtcgc tggtctatcc
     3961 cctggcgcgc ggtaccgggc cgacgctggc caccatcggc gccatcgtca tcctcggcga
     4021 gcaccccggg ccgatcgcgc tggccggcgc ggcgctggtg gtcgttagtg tcttcctgat
     4081 caccggcggc ttcgccgccc cgcgccgcgg cagcgccgcc gcccgcccgg ccatcgccct
     4141 cgggctggtg accggatgct tcatcgccag ttactcgctg tgggacaagc acgccgtcag
     4201 cgcgctcgcg gtgccgccgc tcgtgctcga ctggagcacg tcgctcttcc gcgcgatcgt
     4261 gctctcgccg gtcgcccggc gccgctggtg cgaggtgcgg ggcatctggc gggcgcatcg
     4321 ctgggagacg ctgggcgttg gggtgcttag cacgctggcc tacctgctgg tgctcttcgc
     4381 gctcagcttt accccggtca gtcgcatcgc cccggcgcgc gagatgagca tcctggtcgg
     4441 cacgttcttc ggcacgcgcc tgctccgcga agggaggggg caggtccgcc gtcggctcgc
     4501 cgcggcctcg ctgatggtcg ccggcgtcat cgccctggcg ctcggctgat cgccgcgcga
     4561 tttcgcggca ggggtattga cagccaggca gaagcgtgct actgtgtacg tgcggtcccg
     4621 aggagtgatc ccacggatcg acgccaaaac cttttgtcgt tcggataacc tccgaattga
     4681 ctgcgcagga cgggcaaccg tttctgtatt gtgggtactg gtccaggcga caagcgcaag
     4741 cttcttgttt ggtcaggaga acacgttgag gatggcgtcg gacgcggtag ctcgtcggga
     4801 tcgcgttttt gtgccctgcg acctcactcg actcctccct tggccggtct ggccggcgcc
     4861 tccaactgac gcatggctgg gatcagcggc aggctgagcg attgcgccag cgtggctccc
     4921 gccagcaccg ccaacgcgcc catcagcccc agcccgccga tcaacgcgcc gccgagcagg
     4981 gtgcccagcg gcagcgtgcc gttgctcagg gcgttgatcg tgccgaagac gcggccgcgc
     5041 agcgccgccg gcgtgtgctc ttgcaggatg accagcgaga gtgggttgag tggctcgaaa
     5101 aaggtggcgt tgagcacgat cgagccgagc aagatgggca gctccggccg cagcagcggc
     5161 acgaagaagg ttcccgcccg caccaagaac cccaccacca cgaccagctc acggggcagc
     5221 cgctggccga ccagcgcgta gctcagcagc cccagcagcg ctccgacgcc cagcgccgag
     5281 aacatcaggc cgaggtcgac cgcgctgccg tagacctctt tggcgtagac cggcaggatg
     5341 atggtgtaga aaggctcgct gatcacgctg gccaccgcca cgatcagcac gacccacaac
     5401 agcacgcgat cctggcgcag gaagtggaac ccggccagcg tctcacgcca atacgagtcg
     5461 cgggcgagcg actggtgccg ccgcgtgaca cgcccggcga tggcgatgct gaagatcgcc
     5521 gcggagaacc agaagctgag cccatcgagg tagagcacgt tgctcacgcc catgaacgcg
     5581 atcagaatgc cggcgatcgg cgggccgaac aacagcgcca gccgctgcgc gccgccggtc
     5641 aggctattgg cgcgttcgag cgatgtgccg gcctgctcgg ccaggtcggc caccaggctc
     5701 atgcgggccg ttgcacccgg attgaccagc atcccgcgca agaagacgag cagcaacagc
     5761 gcccagaagt cgagcccgac cgtgtggtag aggagcggga tcaacgccat cgtcagaccg
     5821 ccgcccagat cggacagaat actcgagcga acgaagccga gccggtcgac cagcgcgccg
     5881 ccgacgatgc cggtgatcag aatcggcagc gtgtagaagg ccagcgtgat gccggttctg
     5941 gccgcgctgc cggtcgtaac cagcacgaac caggggatgg cgacggcggt gaaattgctc
     6001 cccagcgcgg agatgaagcc ggccgccaac aggccgagga acggcaggcg ctgtgacatg
     6061 gtcgtgccgc gcgcttcgac ctcatctcgg tgctcgctcg ccacctgctt cgcgttcatg
     6121 agcgcaaacc ttcccccacc gcgccgccga gcgcgacgcg gaccatgtcc cgtctgtgga
     6181 tggtgttagt agaggtccag ctcgccgcgc cggcggacca gccgctcgat acggccgaag
     6241 ctccaccagc cgaggagcgg caggacgacg gcgaagccgg ccagcggcac gagatcgaag
     6301 cgcacctggc tcagcgacgc gtggtgcaac gcggcggccg agagcgcctc catgccgtag
     6361 cccatgggca gcgcgcgcgc cgggatacgc agggcatccg ggagctgact gaccgggtag
     6421 tagctgccgc ccagcagcat caccgccggg aagacgaggt tggccagctc gttcgatttg
     6481 cggatcaaga gcccggcggc gctgaggatc aggcccagcc cgagcagcga cgccaggaac
     6541 agcgccaacg tgaccgccag cgtcagcagg tttggatcga agcgcacccc gaagaggagc
     6601 atgccggaca ccccaaccgt caacgccgcg cccgccgtct cgacgatccc gctggcggcg
     6661 aagccgagca accagctcag ccgggcgcag ggcgccagga agagcatcac cagcgtcccc
     6721 ttgcgccgct cccggtcgag gatctcgccg atccagtaga gggtgttgaa gactgccgaa
     6781 ttggcggtca tcgccactac caggtagggg atcagatccg tacgcccgcc ggcgtagatc
     6841 agggccgcca gcgccaggtc gaagatcggg cgcaccaccc agatgaagag gtagccctgg
     6901 cggccgaagt gctcccagcg ctccttgagc agtacctgcc aggaggcgaa ggccgcgccc
     6961 gcctcagtcg taatcgagcg tcgcgccgcg cttggcgtca tgctccactc tccctaagag
     7021 cagcgtcccg gccagcacca ggacggcgga catgagcagg agtcgcagca gcgggccgcc
     7081 gatgtcgccc agccccgctc caccgagcag cgccgcgcgc agcgcggtca tcccgtcgct
     7141 gatcggaaag acgctcgcca gcgggcgcag cggcccgggt agggcggcga ccggcaccag
     7201 catgccgctc aggagaaaga tcgggctctg caggctgttg gccagcaggt tggcgcgccg
     7261 ggtcagcacg aagaagccgg ccagcgcgta gccgagggcg aacgaggccc ccaccagcgc
     7321 caccagcgcc accagcgccg ccagcgggtc cgtgaccagc agccgcgccc cgctcagcca
     7381 ggccagcagc cccacgacca gcgcgctcgg cagcacgtag agcgcggtcg agccgagccc
     7441 gtagccgagc accacggcgg cgcggctggc gggggtcagg aagagcgcgg tcagcgtccc
     7501 ctccgagcgc tcgtactcga tggcatagcc gctcccccag agattggacg accagagggc
     7561 cagcccgaag gtgccgacca gcaggtagcc ggccacatcg tagccgtcga ccgtcgagcg
     7621 gccagccacg cggtaggtca gcagcatcgt cagaaagagg accgccggga agagcggcca
     7681 gcgcaccagg tcgatcaggg acgagatcgt gcgccgcacg tagcacccgg cgctggccag
     7741 gcagatgtcg gcgaagctcg cctgccctgc cggccgcggg tagaccggtg gcaccggctg
     7801 cggcggctcg ggcgtgcgca ggaaggcgac gatcacgcgg tcttgcatgc ctcggcccct
     7861 tccgtcagcg ccaggaacgc atcctccagc gtcggctcca gcgtctcgac cgcggtcgtg
     7921 cgcattcccc ggccgtggat gatctgcatc gcctcgtcca gaatcggcgc gtcgggcgca
     7981 cagcgcaggg tgtagaccca gccgtcgtcg gcgcgggcgc cggccagctc gcgcaccgcc
     8041 ggcagtgccc gcagcgcatc gcgcaccgcc ggatcgtcgc cgcgcacggt gaagcggtag
     8101 cgctggtcgc cgccgacgcg ccgcttgagc ccggccggcg tgtcttcgac cagaatccgt
     8161 ccctggtcga tgatcgcgat acggtcgcac agctcctcgg cctcgaagag atagtgcgtc
     8221 gtcagcagca ccgtgtgcgc cggcaccagc gactcgacaa tggcgcgcag gttgaccgcc
     8281 gcggccgggt cgagcccgat ggtcggctcg tcgaggaaga tcacgggcgg gtcgtggagc
     8341 agcgcgcgcg ccaggtgcag ccgctgcttc atgccgcgcg agtaggtttc gacgcgctcg
     8401 tccgcccgat cggacagacc caccagcgcc agcagctcgt cgactcgtgc cgccggttgc
     8461 tcccacagcc cgtagaggtg cgcgaagtag cgcaggttgt cacgcgccga gatcttggcg
     8521 tagagtccgc ggtcgccacc cagcagcacg ccgaggcgac ggcgcacctc gcgctcctgg
     8581 cgtacgacgt catagccggc cacgcgcgct cggccaccgc tagggatcag cagggtcgag
     8641 agcatcttga tcgtggtggt cttgccggcc ccgttggggc cgagcagccc gaagatcgtg
     8701 ccgcgctcga ccgccagatc aaccccatcg accgccacgc gctcggggcc gcgactcagc
     8761 agccgccgct tgggacggaa gacgcgccgt agtccctctg cctcaatagc cagcatcgtc
     8821 gtctcctccc gtgctcgaac gcgacggtga gctgaggccg gcccggtcga gcgcatccag
     8881 caggcgcgcc gcctcggccg gcgagatcga gccggccgcc agcgcctcca gcactcgtcg
     8941 gcgtccgccg ttgccgggct gctccgtctg ctccggctgc gggccactcg tctccggtgc
     9001 cgcggccgcc gctgccacgg gctcgccggg cgtcgccggc gcggcgggag ggccgagcgg
     9061 cacgaccggg ctgaccggct gggacggcgg ctgcggccgc gctggtggtt gctcctgtgg
     9121 attcgctggt gtttgcagcc agcgcagcgc gatgtcgccg ctgccgctcc gcagcgagat
     9181 gtcggcgcgc tcggcgccct cgccaacgct gccgaccagc cgccggccga agaggctgcg
     9241 cttgccgcgc tgccccacct ccaccagcgg gatctccgaa tcgatccggc cgcggtgggt
     9301 cgtggcctcg atccgcgccg agatgtcgcg cggcacgctc agttcgatgt cgcccgaacc
     9361 gctctcgatc gagtgctcgt ggagaccaag gcgacagtcg cagtcgacgt cgccggcacc
     9421 ggtcacgatc gcgagcgagc cggcgcggcc gtgcttcagc gtgatgtcgc cgctgcccgc
     9481 cttgatcacg acctcctcgc agtcgcgctc gctcacgtcg acatcgccgg agccggtggt
     9541 gagcgtcagg tcgccgcgcc cgcccagcag ctcgatgtcg ccgctcccgc cggtcagcgc
     9601 gatcttgccc accacctgcc gcagctcctt ctgacccgag ccgccggtga tcgagagagg
     9661 gccctggtat tcctcgacct ccaggtcgcc gctgccgata gcgatcgtgg cctgtccctg
     9721 gccgccgtgc atgaccagat cgccgcgatc gaggcgcaag tcgcactggc cgcgcgcatc
     9781 gctcaggcgc acgttgccct ggcgcacccg caggcgaaag gtgggcagcg tgcgcggcag
     9841 acgcaggcgc accgtctcgc cgctgccgcc tccctgggcg acaaaggcga tctggccaac
     9901 cgagatgcgc acaccgtgcg gcgctccggc gccgccgaca atcaattcgt cgccggcggg
     9961 ctcggccgcc acagcctccc cctgcacggc gatcacgtca gactcgaccc attcgacact
    10021 gacgctccgc cgctccagtt cgaggctgac gcgctcgatc ccctcggcgg gaaacgtctg
    10081 ctcgctcaca ctcatgattc ctccgtcagg ctgccgagcc gcgccaggcc ttcatcgact
    10141 cctacctcgc cgcgcgcgat gcgctggagc agctcgtccc gcgcgctcgg tctggttgtg
    10201 gcggcgggcg gcggcggggc ggctggcggc tcctgatcgc ccagtaactc gatcacttct
    10261 tccaacttgg cgcgcaccgt ggggtaggag acgccgagcg tgcgctcgac ctcgctcaga
    10321 ttgccgcggc tacgcacgaa gaggcgcagg aaggtctgct gctcgggcga cagccggctg
    10381 aaggggccgg cggcccagcg accacgcacg accgtctcgc aggccgggca actgagctcg
    10441 cgcagctcca gggggtgacc acaggtcgga cattgttggg gcgctggaaa catgcgccac
    10501 ctcctttccg gcccatccta ctcgccgcga ttaccaatgt caatagatca atgactgttc
    10561 ctcataatcc agtgaccatc tctgaatact gtgctcgcgg cccgcgtatc tcagcgaatc
    10621 ctccagaccc cctccatgcg gaacgaattg catggcgtcg attctgcgat cctgcccggg
    10681 ggttgctcgc cttcaaagag caggcgcaac gcgaaagagc ggcacgatga agcgaccgtc
    10741 gacccagcgc caacggatcg gcccggctgt ccggcgtttg cgccggcagc agggtctgac
    10801 gctggacgcg ctcgccatga cagccgaggt ctcgccctcg catctcagcc ggctggagcg
    10861 cagccagacg ctgccgagct tcccggtatt ggccaagatc gccgaggcgc tgggggccga
    10921 tgtcaacgag ctggtgcgcc tggagcacga cgtcgcccag ctcgatgccg agctggaggg
    10981 ctacctcgac atgctcggca tcgcggccga gcagcgcgcg gaaatcctgc acctctcaat
    11041 cgagacgcgc cgcgatctca ccggccgcct gcgcgcgctg gccgaggatc gcctgactcc
    11101 gctcgcgctg caggacacga tcgcccgggt gctctgccag ccgggcgggc tgccggggct
    11161 cgcccagacc gcccgctaca tctgcgactc gggcctcagc ggtacgggct acgcccgggc
    11221 gctgctggag ttggatcagt tgctcgggcg gcggcgcctg ctggccggcg gcgccagcct
    11281 gctgccgctc gcgcccggca cggcgctgct gccgcacttt cgccacgtct tcggcagtga
    11341 gccgctcgat cccgcggtcg cgacctggtg gctggagccg caccagaacg cgtacccgcg
    11401 cggcacaccg caccgtccgt tgcgcgcgat tctcggccgc cgcgccctcg agtctccgct
    11461 tggtccggtc atggcgcgct cgctcctgga agtgatcgcg cgccccgagg gagaggtcgc
    11521 gttgaccgag cgcgaacttg gcccggtcaa cgcgctcgcg gtcgatggca gctatgcctt
    11581 gctggaggat ctaccgggtg agccggagac cgactgctgc gcggccctct ggctggccgg
    11641 tcaacagcga gtggcaccgc tcgatcacct gatcgacagc ctctgggatg ggctgccgcc
    11701 ccacgagaag tatcccacgg cggtcgctga ggccttgcgc cgcgccgccc gcgcctagct
    11761 ccagcgaccc tcgacggtct ctcgacagcc cctcgaccgc atgtgccccg cccgcatcta
    11821 gactcaatcc agagcgggga cgacctctgc gcgtcttgca cctgtccgcg catggcacgt
    11881 gagcagggat ccaacatgta cgacaccttt caaccggttc agccggttca gcaggcgagc
    11941 gcgataccgc tgtcgagcgc ggagccccgg cgtgtctgcc tgctcagcga cgatctctcg
    12001 ggaccaccgg atgagggcgt caagaaggtc acgctgcagc ttgcggcggc gttggcgcca
    12061 caccaccacg tcagccttct ctcgaccact ccgacgagcc agggcagcgc ggcgcgctac
    12121 gtcccggcac cgcgtacctt tctcagccgc tcgctgcggg cggagctgcg gcggctgcaa
    12181 ccggacgtcc tgatctacgc cacgcgccgc tcggccacct tcaattcgtt cctgcgcgcg
    12241 cggctcttga agcgctactg ccccacggct acgttggtgc tcctcgcctt gcagacgcgc
    12301 cggcacccgg cctggcagca gcgcctgatt cgccacctgc tccccgacct cgccggcgtg
    12361 caatcgggcg ccaatcgcga ctatctcacg gggctcggct gccacgtgac gctggtgcgc
    12421 ggcggggtcg acagcgccgc ctttcaaccg gtggacgagg cccggcgaca gctgctgcgt
    12481 gcccgctacg gcctgcggga tgacctgccg gtcgtgctcc atgtcggcca cctgcaccgc
    12541 gagcgcggcg ttggcgtcct cgccgcgctg gccagcggcc acgagtgcca ggtggtgctg
    12601 gtggccagct ccagcacgcc ccaggaggcc gacatcggcg acgagctgcg cgcggcgggc
    12661 gtcacgctgt tgaccaccta cctgccccac atcgaggagc tctatcaact ggccgattgc
    12721 tacgtcttcc cggtgctgag caccgacgcg gcgatcgagt tgcccctctc ggtgctggag
    12781 gcactggcct gcgacctccc ggtcgtcacg acgcgcttcg gcgggctgcc gcaggcgttc
    12841 gccaacgcgc cgagcgaggg gctggtcttc atcgactcgc cgcaagagct cgtgactgag
    12901 gcgctgcgcc tctgtcgcga ggattcagga tcgacacgtc cgctggcgct ccccttcgcc
    12961 tgggaggccg tcgccgccga tctgctggcg caggcgttgg cgattcgcgg ggagccaaac
    13021 ggccgagctg tggaaattga gggcctatca cagggcgaga cgccatgggc gggagaagtt
    13081 tcgcatgccg ggcagtgaag gcgggggcgc gagcccgggt actgtggccg atgcggtcgt
    13141 ggtcgcggag gcgcctcgcc gctgggcgac aaacgccgcg cccgatgcgc gcggcggccc
    13201 ggaagtcacc ctcctggtgc ggctgctgga tgagggcgaa cgcgcgacgc cggcacccgg
    13261 cctcgacccg gcgcgcttca tcgactggtg ccggcgcaac aagttcccgc tgctctcttt
    13321 ggagcgccac accccggcct ggttggccga ggacccggat ttcgcgcggg cgctggcggc
    13381 agaatccgac tggttcgcca cccaacacgg cgagtaccac cggttgcgcg acgcctggct
    13441 ggcgcgcggc atcccctgcc tgctgatcaa atccgccggc gaacatccgg ccttcccgca
    13501 cagcagcgat aacatcgata ttctggtgca tccggagcag gggctggcgg cgcgcgagac
    13561 gctgcgcgat ctcggttacg ttgagctgcg caacatcgaa gagccgggca agtttctctt
    13621 tcgccgcttc agcgacggtg tctgtgtctc ggccatccat ctccacgagc gcatcgcctg
    13681 gttcgtcggc tttctcgacg accaggccgt ctggcggcgc gcccgcgcgg cgcacgacga
    13741 tccggcggtg accatcccag cgtccgagga cgccgtcctg atcaacctgg cgcacgcct
//
LOCUS       DASUBI010000723         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_754910, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000723 DASUBI010000000
VERSION     DASUBI010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754910"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..3722
                     /gene="dnaE"
                     /locus_tag="VFU72_14090"
     CDS             150..3722
                     /gene="dnaE"
                     /locus_tag="VFU72_14090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5424670.1"
                     /translation="MSDSFVHLHVHTEYSMLDGAARLKDMFKHAAELGMPAVAMTDHG
                     NMHGAADFTKQANAAGIKPILGIEAYVAPESRYNPKPIRWGQPHQKSDDVSGGGLYGH
                     ATIWAKDVTGLQNLTKLSSRAFTEGFARKYPRMDAELMAEHSAGLMATTGCPSGFVQT
                     RLRLGQFDEALAAAAQYQEIFGKENFYLELMDHGLDIETKVRQGLLDIGRKLDIPPVV
                     TNDSHYTYQHESAAHDALLCIQTASNIADPDRFKFGGDGYYLKSADEMRAVSPTDEIW
                     LEGCRNTLLIAEKVNPEGMFSYRNLMPRLDPPDGLTEDQWFRQLAYDGLNRRFPAGIP
                     DEKVYHARLEFELDVILSMGFPSYFIVVADFINWAKNNGIAVGPGRGSAAGSLVAWSL
                     GITDVDPIPFDLLFERFLNPDRVTPPDIDIDFDDRRRSDVIRYVTEKYGEDKVAMIAT
                     FGRIKAKAAIKDASRILGYPFAMGDRITKAMPPDVMGKSMPLSGVFDSNHPRYGEAGE
                     IRGMYEQDADVKKVIDTGRGVEGLIRQPGVHAAGVIISPETITDHVPVMMRPADGVII
                     TQFDYPTCEEIGLIKMDFLGLRNLTIITDALNNVETTTGKRLNTLEIPLDDPLTYELL
                     CKGDTLGVFQLDGGPLRSLLRQMRPDNFEDVSAIIALYRPGPMGANSHINYAERKNNR
                     QPITPIHPELEEPLKEVLGVSYGLIIYQEQVMAAARVLAGYSLAQADLLRRAMGKKKK
                     EVLDKEYVGFEKGMADNGYSAQATKAVWDVLVPFADYAFNKSHSVAYGFIAYQTAYLK
                     AHYPAEYMAALLTSTATDKDRSAIYLAECRRMGIKVLPPDVNDSLHQFTPVGTDIRFG
                     LGAIRNVGENVVSSIIATRKAKGRFTSFNDFLAKVDLPVCNKRTIESLIKAGAFDSLG
                     HSRRGLIEQHERAIDSVVEVKKQEALGQDSLFGSLMDGGQEAITGGVEMTFSEIEWDK
                     KVLLGFEREMLGLYVSDHPLSGVENLLASKADCSIASLTGENDRADGSIVTIAGLVAG
                     LARKVTKQGNTWAAASIEDLEGGIETMFFPQTYQLYSTQLAEDAVVVVKGRLDKRDEV
                     PKLIAMEVAMLDISAAGSSAPVVLHVSEARVIPATVERLKRILTNHPGTTPVRMHLKS
                     RDDRVIRYQLGDNLRVANSPALSSDLKVLLGAGCFGSDPSQSAE"
     gene            3726..>4264
                     /locus_tag="VFU72_14095"
     CDS             3726..>4264
                     /locus_tag="VFU72_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5424671.1"
                     /translation="MEFQDVIRKRRMVRAFTTEPIAPEATERLLRAANRAPSAGFSQG
                     YAMLVLESAEDRMSFWTAMDPEGEYEGTPLRNAPLVVIPCSNKEIYLDRYARTDKGWS
                     DRDEARWPVPFWHIDTGMLTMLLLLATVEEGLGALYFGIPPESIGNVRAEFGIPDDFT
                     PIGAVAIGHPDPERDARQKT"
BASE COUNT          762 a         1490 c         1392 g          620 t
ORIGIN      
        1 ggaggcgcga cacgcccggg tttccggcgc gcactccggt ccaccagcag ctcggccacc
       61 ggcctgtcgg cagcagccgc ccggccggcg ccgaccggat tagactctcg atgccccgcc
      121 cgaaccagcg cgtccgcagg aggatccctg tgtctgattc cttcgtccac ctgcacgttc
      181 acaccgagta ttcgatgctc gacggtgcgg cgcgattgaa ggacatgttc aagcacgccg
      241 ccgagttggg catgccggcg gtggcgatga cggaccacgg caacatgcac ggcgcggccg
      301 acttcaccaa gcaggccaac gccgccggca tcaagccgat cctcggcatc gaggcgtacg
      361 tcgcgccgga gtcgcgctac aacccgaagc cgatccgctg gggccagccg caccagaagt
      421 ccgacgacgt ctccggcggc ggcctgtacg ggcacgcgac catctgggcc aaggacgtca
      481 ccggcctgca gaacctcacc aagctctcct cccgcgcctt caccgagggc ttcgcgcgca
      541 agtacccgcg catggacgcc gagctgatgg ccgagcactc ggccggcctg atggcgacca
      601 ccggctgccc gtccggcttc gtgcagaccc gcctgcgcct gggccagttc gacgaggctc
      661 tggcggcggc cgcgcagtac caggagatct tcggcaagga gaacttctat ctggagctga
      721 tggaccacgg tctggacatc gagaccaagg tccgccaggg cctgctggac atcggccgca
      781 agctcgacat cccgccggtg gtcaccaacg actcgcacta cacctaccag cacgagtccg
      841 ccgcgcacga cgcgctgctg tgcatccaga ccgcctcgaa catcgccgac cccgaccgct
      901 tcaagttcgg cggcgacggc tactacctga agtccgccga cgagatgcgg gcggtcagcc
      961 cgaccgacga gatctggctg gagggctgtc gcaataccct gctgatcgcc gagaaggtca
     1021 atccggaggg catgttctcc taccggaacc tgatgccccg cctggacccg ccggacggcc
     1081 tgaccgagga ccagtggttc cgccagctcg cctacgacgg tctgaacagg cgcttcccgg
     1141 ccgggatccc ggacgagaag gtctaccacg ctcggctgga gttcgagctc gacgtcatct
     1201 tgtcgatggg cttcccgtcg tacttcatcg tggtcgccga cttcatcaac tgggccaaga
     1261 acaacggcat cgcggtgggc ccgggccgcg gatcggccgc cggatcgctg gtcgcgtggt
     1321 cgctgggcat caccgacgtc gacccgatcc cgttcgacct gctcttcgag cggttcctga
     1381 accccgaccg ggtcaccccg ccggacatcg acatcgactt cgacgaccgc cgccgctcgg
     1441 acgtgatccg ctacgtcacc gagaagtacg gcgaggacaa ggtcgcgatg atcgccacct
     1501 tcggccggat caaggccaag gcggcgatca aggacgccag ccggatcctg ggctatccgt
     1561 tcgcgatggg cgaccgcatc accaaggcga tgccgccgga cgtgatgggc aagtcgatgc
     1621 cgctgtccgg ggtgttcgac agcaaccacc cgcgctacgg cgaggccggc gagatccgcg
     1681 ggatgtacga gcaggacgcg gacgtcaaga aggtcatcga caccggccgc ggcgtggagg
     1741 gcctgatccg gcagcccggc gtgcacgccg ccggcgtcat catctccccg gagaccatca
     1801 ccgaccacgt gccggtcatg atgcggccgg ccgacggcgt catcatcacg cagttcgact
     1861 acccgacctg cgaggaaatc ggcctgatca agatggactt cctgggactc aggaacctga
     1921 cgatcatcac cgacgcgctg aacaacgtcg agaccacgac cggcaagcgg ctgaacacgc
     1981 tggagatccc gctcgacgac ccgctgacgt acgaactgct gtgcaagggc gacacgctcg
     2041 gggtgttcca gctcgacggc gggccgctgc gctcgctgct gcgccagatg cggccggaca
     2101 acttcgaaga cgtctcggcc atcatcgccc tgtaccggcc cgggcccatg ggcgcgaact
     2161 cgcacatcaa ctacgccgag cgcaagaaca accggcagcc gatcaccccg atccacccgg
     2221 agctggaaga gccgctgaag gaggtcctcg gcgtcagcta cggcctgatc atctatcagg
     2281 agcaggtgat ggcggccgcg cgggtgctgg ccgggtactc cctggcccag gcggacctgc
     2341 tgcgccgggc gatgggcaag aagaagaagg aagtcctcga caaggagtac gtgggcttcg
     2401 agaagggcat ggccgacaac gggtactcgg cccaggccac caaggcggta tgggacgtcc
     2461 tggtcccgtt cgccgactac gcgttcaaca agtcgcactc ggtcgcctac ggcttcatcg
     2521 cgtatcagac cgcttatctc aaggcccact atccggccga gtacatggcc gcgctgctca
     2581 cctcgacggc caccgacaag gaccgctcgg cgatctacct ggccgagtgc cgccggatgg
     2641 gcatcaaggt cctgccgccg gacgtcaacg actcgctgca ccagttcacc ccggtcggca
     2701 ccgacatccg cttcggcctc ggcgcgatcc gcaacgtcgg ggagaacgtg gtctcctcga
     2761 tcatcgccac ccgcaaggcc aaggggcgct tcacgtcctt caacgacttc ctggccaagg
     2821 tggacctgcc ggtctgcaac aagcggacca tcgaatcgct gatcaaggcg ggggccttcg
     2881 actccctggg ccactcccgg cgcgggctga tcgagcagca cgagcgcgcg atcgactcgg
     2941 tggtcgaggt caagaagcag gaggcgctgg gccaggactc gctgttcggc tcgctgatgg
     3001 acggcggcca ggaggcgatc accggcgggg tggagatgac cttctcggag atcgagtggg
     3061 acaagaaggt cctgctcggc ttcgagcgcg agatgctggg cctgtacgtc tcggaccatc
     3121 ccctctccgg cgtggagaac ctgctggcgt ccaaggcgga ctgctcgatc gcctccctga
     3181 ccggcgagaa cgaccgcgcc gacggctcga tcgtcaccat cgccggcctg gtcgccggac
     3241 tggcccgcaa ggtcaccaag caggggaaca cctgggccgc ggcctcgatc gaggacctcg
     3301 aaggcggcat cgagacgatg ttcttcccgc agacctacca gctgtactcc acgcagctgg
     3361 ccgaggacgc ggtggtcgtg gtgaagggcc ggctggacaa gcgcgacgag gtgccgaagc
     3421 tgatcgcgat ggaggtcgcg atgctcgaca tctccgcggc cggcagcagc gccccggtgg
     3481 tgctgcacgt cagcgaggcg cgcgtgatcc cggcgacggt ggaacgcctc aagcgcatcc
     3541 tcaccaacca ccccggaacc acgccggtcc gcatgcacct gaagagccgc gacgatcgcg
     3601 tgatccgcta ccagctcggc gacaacctgc gggtggccaa ctccccggcg ctgagttcgg
     3661 atctcaaggt tctgctgggc gccggctgct tcggctcgga cccctcgcaa tcggcggagt
     3721 gagccgtgga attccaggat gtgatccgca agcgccgcat ggtccgggcg ttcacgaccg
     3781 aaccgatcgc cccggaggcc accgagcggc tgctgcgagc ggccaaccgc gcgccgtcgg
     3841 ccggcttctc ccagggctac gcgatgctgg tgctggaatc ggccgaggac cggatgtcgt
     3901 tctggacggc gatggacccc gagggcgagt acgagggcac gccgctgcgc aacgcgccgc
     3961 tggtggtgat cccctgctcc aacaaggaga tctacctcga ccgctacgcc cggaccgaca
     4021 agggctggag cgaccgcgac gaggcccgct ggccggtgcc gttctggcac atcgacaccg
     4081 gcatgctgac gatgctgctg ctgctcgcca cggtcgagga gggcctcggc gcactgtact
     4141 tcggcatccc gccggagtcg atcggcaacg tccgcgccga attcggcatc ccggacgact
     4201 tcacaccgat cggcgcggtc gcgatcggac acccggaccc ggagcgcgac gcgcggcaga
     4261 agac
//
LOCUS       DASUBI010000724         7497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_249801, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000724 DASUBI010000000
VERSION     DASUBI010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249801"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="trpD"
                     /locus_tag="VFU72_14100"
     CDS             complement(<1..990)
                     /gene="trpD"
                     /locus_tag="VFU72_14100"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5424672.1"
                     /translation="MTATMEQAAGQAGQEIIRAAIRTVAEGALLSRAEAAGAMDAIMS
                     GAATGAQIGALMTALRMRGESDEEIAGFAEAMRGHALRVEPSHAGPVVDTCGTGGDGA
                     HTFNISTTAAFVVAGAGVPVAKHGNRAASSRCGSADVLEALGVRIDLAPEAVARCIDE
                     AGIGFMFAQVFHPAMRFAGPARAEIGIRTVFNILGPLTNPAGARHQVVGVPTPAVGEK
                     VARVLGLLGTNHALVVHSGDGLDELGLSAPNQISEVRSNGSVSVRTYELRASDVGLEA
                     ATVEAIRGGDAEDNAALVRHVLGGGNGAPRAVTLLNAGAALYAADAVDSIPAGI"
     gene            complement(992..1585)
                     /locus_tag="VFU72_14105"
     CDS             complement(992..1585)
                     /locus_tag="VFU72_14105"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5424673.1"
                     /translation="MILLIDNYDSFTFNLYQYLRELGAEVQVRRNDEVCVDEVRLLDP
                     ERIVISPGPCTPHEAGVSVELVRQLAPALPILGVCLGHQAVGAAFGARIIRAPKLMHG
                     KTSRITHDRQGVFAGLPSPFTATRYHSLVVDEATLPEELAVSARSEDGLIMGLRHREY
                     PVEGVQFHPESILTEHGRDLLANFLGRPTPVLAIRGY"
     gene            complement(1582..3159)
                     /gene="trpE"
                     /locus_tag="VFU72_14110"
     CDS             complement(1582..3159)
                     /gene="trpE"
                     /locus_tag="VFU72_14110"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU5424674.1"
                     /translation="MLDLRRVPEAPTGREPAGRYHPTLDEVAALAERANIIPVYREIF
                     ADLETPVSAYLKVARGPYSFLLESIEGGERLGRYSFIGADPYLTLRLDQGVAEANLRG
                     YRQTAAFSDPLVAVQEFLAPYQAVELPGLPRFVGGAVGFLGYDAVRYFERLPAPPHDS
                     TGFPEAFFMFVDSMLVFDHLRRAIKVVSHVRVDEHTPLERSYAEAVARIDELAARLQG
                     EPAIPVGAAPLDVEPVEARLRRHTTPERYQAMVRAAKEYISAGDIIQVVLSQRVSLPT
                     AAHPFSVYRALRQVNPSPYMFYLQLGEAQIIGSSPELLVRLDGDTLTTHPIAGTRPRG
                     ADEAADAALAEELAGDEKERAEHIMLVDLGRNDIGRVAEPGTVRVPQLMEIERFSHVM
                     HLVSHVTGELRPELRGLDALRACFPAGTVSGAPKIRAMEIIAELEEERRGPYSGAVGY
                     VDFGGNLDTCITLRTLWMRDGVAQFQAGAGLVADSDPVSEDAECFHKLRALVRAVELA
                     EAIERDARSTQEREAEA"
     gene            complement(3517..4032)
                     /locus_tag="VFU72_14115"
     CDS             complement(3517..4032)
                     /locus_tag="VFU72_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEU5424675.1"
                     /translation="MSDSRSSAQPTQVNDILHLIDRLEEVAASATRVPFGGRIVVDEG
                     ELLDIIDQLRLSIPQEIKQARSVVRERHQIIAEAQTEAERILSIAKERAEYLMSEQGL
                     VNEARARGEEILRDARDKEKRSMGQVDLYALQILSQMEQVLERNLEQVSQSLEEVRQA
                     KDTVAAPAARP"
     gene            complement(4060..4563)
                     /gene="coaD"
                     /locus_tag="VFU72_14120"
     CDS             complement(4060..4563)
                     /gene="coaD"
                     /locus_tag="VFU72_14120"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5424676.1"
                     /translation="MERLAVYPGSFDPITNGHVDVAERAARIFDTLIIGIYESDAYGS
                     KQPLFSAEERRRLAESAVQDIPNVRVDIFRGLAVDYARSVGAKTIVRGLRAVSDFEYE
                     FKYAHMNRHLAPEIDVICLMTSSQQSFISSSFIREVASLGGDVRGLVPNLAADALEQK
                     FGAISRK"
     gene            complement(4587..5108)
                     /locus_tag="VFU72_14125"
     CDS             complement(4587..5108)
                     /locus_tag="VFU72_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU5424677.1"
                     /translation="MRTRPMADKIKEAAYSMLASLGVQPRRVLDLYAGSGGVAIEALS
                     RGAESADFVEQNPAACAVIRDNLAATGFAEQGRVFQSSVAAFLAHVREPYDFIILDPP
                     YADPLIPATLQTLADSPAVADGTVLLLGHSPRVELPERAGRLQRLRDRCHGDSCFAIY
                     EAGAVERPRAETE"
     gene            complement(5143..6009)
                     /gene="pheA"
                     /locus_tag="VFU72_14130"
     CDS             complement(5143..6009)
                     /gene="pheA"
                     /locus_tag="VFU72_14130"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5424678.1"
                     /translation="MRIAYLGPMGTFSEEAALAQSRRDGSEPMPFASIPALVSAVETG
                     LAERAILPIENSLEGSVSATLDLLIHETSLKILAELVVAVRHNLVAVPGTELRDISVV
                     LTHPQVPGQVRRFLDRCLPNVTQVAALSTAAAVEEVVRGGDRSRAAIGTTRAAELFGG
                     EILARDIQDNRGNVTRFFVLGREDAAPTGNDLTSFCFRVKANVPGALYEVLAELAEAN
                     IQMTKVESRPTKSGLGDYFFLVDIEGHRLDPAIAAALGRIREKTALLKIFGSYPHSVN
                     GTNGTVDAGDQW"
     gene            6186..6878
                     /locus_tag="VFU72_14135"
     CDS             6186..6878
                     /locus_tag="VFU72_14135"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5424679.1"
                     /translation="MISDSEIVLRLAISAALSGVVGLERERLEKSAGLRTHALVGVGA
                     TLVMIVSAFGFADVLGTPDVVLDPSRMAAQVVSGIGFLGAGTIIFRRQIVQGLTTAAS
                     IWAVAAIGLAVGGGMYLAAAAATVLALLILAGIKPIEHRIFTQRQPPLLTLLVDRRPG
                     TLPTIEAVIQSAGVGIEQIVIEPGAEDHEDRVELTLTQPHSGAALIPLLETLRHIPGV
                     REIRSASSTPPG"
     gene            7051..7284
                     /locus_tag="VFU72_14140"
     CDS             7051..7284
                     /locus_tag="VFU72_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424680.1"
                     /translation="MAHIPKDDIQQLWNELPSKNWSGLHKVLQNHQGKANGISNNLVD
                     DMLRMSQRNEQSGKAFPGSMDQLYQQLNSELQS"
BASE COUNT         1140 a         2734 c         2477 g         1146 t
ORIGIN      
        1 gatgcccgcc gggatcgagt cgaccgcgtc ggcggcgtag agcgcggcgc cggcgttcag
       61 cagcgtcacg gcgcgtggcg ctccgttgcc gccgccgagc acgtgccgca ccagcgcggc
      121 gttgtcctcg gcgtccccgc cccggatcgc ctccaccgtc gcggcctcta gcccgacatc
      181 gctggcgcgc agctcatagg tgcgaacgct cacgctgccg ttcgagcgca cttcactaat
      241 ctggttcggc gcgctcaacc ccagctcgtc cagcccgtcg cccgagtgca ccaccagcgc
      301 gtgattggtg ccgagcagcc ccagcacccg cgccaccttc tcgcccaccg ccggggtcgg
      361 cacgccgacc acttgatggc gcgcgccagc cgggttagtc agcgggccga ggatgttgaa
      421 gaccgtgcgg atgccgatct cggcgcgggc cgggccggcg aagcgcatgg ccggatggaa
      481 gacctgggcg aacatgaagc cgatgccggc ctcgtcgatg cagcgcgcca ccgcctccgg
      541 cgccaaatcg atgcgcaccc ccagcgcctc cagcacgtcg gccgagccgc agcggctgct
      601 ggcggcgcgg ttgccgtgct tggccaccgg cacccccgcc ccggccacca cgaaggccgc
      661 cgtggtcgag atgttgaagg tgtgcgcccc gtcgccgccg gtgccgcagg tgtcgaccac
      721 cggtccggcg tggctcggct cgacccgcag cgcgtggccg cgcatcgcct cggcgaaacc
      781 ggcgatctcc tcgtcgctct cgccgcgcat gcgcagcgcc gtcatcagcg cgccgatctg
      841 cgccccggtg gccgcgccac tcatgatcgc gtccatggcg cccgccgcct cggcgcggct
      901 caagagcgcg ccctcggcca ccgtacggat ggccgcgcgg atgatctcct gaccggcctg
      961 cccggccgcc tgctccatcg tcgccgtcat ctcaataccc cctgatcgcc aggaccggcg
     1021 tcggccggcc caggaaattc gccagtagat cccggccgtg ctcggtgagg atcgactcgg
     1081 ggtggaactg caccccctcc accgggtact cgcggtgacg cagccccatg atcaggccgt
     1141 cctcgctgcg ggcgctgacc gccagctcct ccggcagcgt cgcctcgtcg acgaccagcg
     1201 agtggtagcg ggtggccgtg aacgggctcg gcagcccggc gaagaccccc tggcggtcgt
     1261 gggtgatgcg cgaggtctta ccgtgcatca gcttcggcgc ccggatgatg cgcgcgccga
     1321 aggccgcgcc caccgcctgg tgccccaggc agacgcccag gatcggcagc gcgggcgcca
     1381 gttgtcgcac cagctcgacc gagacgcccg cctcgtgcgg ggtgcacggt cccggcgaga
     1441 tcacgatgcg ctcgggatcg agcagccgca cctcgtcgac gcacacctcg tcgttgcggc
     1501 gcacctgcac ctcggccccc agctcgcgca ggtactggta gaggttgaag gtgaacgagt
     1561 cgtagttgtc gatcagcagg atcatgcctc cgcctcccgc tcctgagtac tgcgcgcgtc
     1621 gcgctcgatc gcttcggcca gctcgaccgc ccgcaccagc gcccgcagct tgtggaagca
     1681 ctcggcgtcc tcgctcaccg ggtcgctgtc ggccaccagc ccggcgcccg cctggaactg
     1741 cgccacgcca tcgcgcatcc agagcgtgcg cagtgtgatg caggtgtcga ggttgccgcc
     1801 gaagtcgacg tagccgaccg cgcccgagta cggcccgcgc cgctcctcct ccagctcggc
     1861 gatgatctcc atggcgcgga tcttgggcgc gccgctgacc gtgccggccg ggaagcaggc
     1921 gcgcagcgca tccaggcccc tcagctccgg tcgcagctcg ccggtcacgt ggctgaccag
     1981 gtgcatcacg tggctgaagc gctcgatctc catcagctgc ggcacgcgca ccgtgcccgg
     2041 ctcggccacc cgcccgatgt cgttgcggcc cagatcgacc agcatgatgt gctcggcccg
     2101 ctccttctcg tcgccggcca gctcctcggc cagcgccgcg tccgccgcct cgtcggcccc
     2161 gcgcggccgc gtgccggcga tcggatgcgt ggtcagcgtg tcgccgtcga gccgcaccag
     2221 cagctccggc gaggagccga tgatctgcgc ctcgcccagc tgcaggtaga acatgtaggg
     2281 cgaggggttg acctgccgca gcgcgcggta gaccgagaag gggtgcgccg ccgtgggcag
     2341 cgagacccgc tgcgagagca ccacctggat gatgtcgccg gcgctgatgt actccttggc
     2401 cgcgcgcacc atcgcctgat agcgctcggg ggtggtgtgc cgtcgcagcc gcgcctcgac
     2461 cggctccaca tccagcggcg cggcaccgac cggaatggcc ggctcgccct gcagccgcgc
     2521 cgccagctcg tcgatgcgcg ccaccgcctc ggcataggac cgctccagcg gcgtgtgctc
     2581 gtccacccgc acgtggctga cgaccttgat cgcccggcgc aggtgatcga agaccagcat
     2641 ggaatcgacg aacatgaaga aggcttcggg gaagccggtg ctgtcgtgag gcggcgccgg
     2701 caggcgctcg aaatagcgca ccgcgtcata gccgagaaag ccgaccgccc cgccgacgaa
     2761 gcgcggtagc cccggcagct cgaccgcctg atagggcgcc agaaactcct gcacggccac
     2821 cagcgggtcg ctgaacgcgg cggtctgacg gtagccgcgc aggttggcct cggccacgcc
     2881 ctggtcaagc cgcagcgtca gataggggtc ggcgccgatg aacgagtagc gccccaggcg
     2941 ctcgcccccc tcgatgctct ccaacaggaa ggagtagggg ccgcgcgcca ccttgaggta
     3001 ggccgagacc ggggtctcca gatcggcgaa gatctcgcgg tagaccggga tgatattggc
     3061 acgctcggcc agcgcggcga cctcgtcgag ggtggggtgg tagcgccccg ccggctcgcg
     3121 cccggttggc gcctccggca cccggcgcag atcgagcatc tcggtgatcc ttccgcgaag
     3181 cctggacaac aaaaaacctc catcccgata agggacggag gctgcatgct ccgcggtgcc
     3241 accctcattg gagcgcgctt ccgcgccgct cccactcaac aggtacggga tcgtgagatc
     3301 cgatacccag ctcccagata acggtggagt ctccggcgcc gcctactggc tctcgcgttc
     3361 ggggcgcggc tcccggatcc attcggcgtc cgcgcgagtg ccagactctc accaaccgct
     3421 ggctctctga actccgcctc gacgcctact cgttccgatc gtcgccgctc ggctcttcga
     3481 ttgtcgtcgt cagactagca aagcccggcg cgggtgtcaa ggcctggcgg ccggcgcggc
     3541 caccgtatcc ttggcctgcc gcacctcttc caggctctgc gacacctgct ccagattccg
     3601 ctccagcacc tgctccatct gcgacaggat ctgcagcgcg tagaggtcga cctgccccat
     3661 gctgcgcttc tctttgtcgc gggcgtcgcg caggatctct tcgccgcgcg cgcgggcctc
     3721 gttgaccagc ccctgctcgc tcatcagata ctcggcccgc tccttggcga tcgagaggat
     3781 gcgctccgcc tcggtctgcg cctcggcgat gatctggtgc cgctcgcgaa cgacgctgcg
     3841 ggcctgcttg atctcttgcg ggatggagag tcggagctgg tcgatgatgt cgagcagctc
     3901 accctcatcg accacgatgc ggccgccgaa cggcacccgc gtcgcgctcg ccgccacctc
     3961 ctcgagccgg tcgatcaaat gcagaatatc gttgacctgc gttggctgcg cgctcgaacg
     4021 gctgtcagac acgccacgtc catcctctgt gctcgttgcc tacttgcgcg agatcgcacc
     4081 gaacttctgc tcaagtgcgt ccgccgccag attcggcacc agcccgcgca catcaccgcc
     4141 cagcgaggcc acctcgcgaa tgaagctgga gctgatgaac gactgctgcg agctcgtcat
     4201 caggcagatc acgtcgatct cgggcgccag atgccggttc atatgcgcgt atttgaattc
     4261 gtactcgaag tccgataccg cgcgaaggcc gcgcacgatc gtctttgcgc cgacggaacg
     4321 cgcatagtct acggccagcc cacgaaagat gtcaacgcgc acgttcggga tatcctgcac
     4381 ggcggactcc gccagccgcc ggcgctcctc ggccgagaag agcggctgct tgctgccgta
     4441 ggcatcgctc tcgtagatgc cgatgatcag ggtgtcgaag atgcgcgccg cgcgctcggc
     4501 cacgtcgacg tggccgttgg tgatgggatc gaacgagccg gggtagaccg ccagtctctc
     4561 cacccgatac ccccagttac tcccagttac tccgtctcgg cccgcggccg ctctaccgcc
     4621 cccgcctcgt agatcgcgaa acaactgtcc ccgtggcagc ggtcgcgcag gcgttgcagc
     4681 cgccccgcgc gctccggcag ctccacacgc ggcgagtgcc ccagcagcaa cacagttccg
     4741 tcggccacgg ccggcgagtc ggccagggtc tgaagtgtag ccggaatcag cgggtccgca
     4801 tagggcgggt ctaggatgat gaagtcgtag ggctcacgca cgtgagccag gaaggcggcg
     4861 accgagctct ggaagacgcg tccctgctcg gcgaagccgg tggcggccag gttgtcgcgg
     4921 atcaccgcgc aagccgccgg gttctgctcg acgaagtcgg cgctctcggc gccgcgtgag
     4981 agcgcctcga tcgccacgcc gccgctgccg gcgtagagat ccagcacgcg ccgcggttga
     5041 accccgagcg acgccagcat cgagtaggcg gcctccttga tcttgtccgc catcggacgg
     5101 gtgcgcatcg tcttgggcgc cttcagccgc cgcccgcgcg cgctaccact gatcacccgc
     5161 atcgaccgtc ccgttcgtgc cgttcacgct gtgcggatag gagccgaaga tcttcaagag
     5221 cgccgtcttt tcgcggatac gcccgagcgc cgccgcgatc gcggggtcga gccggtgccc
     5281 ctcgatatcg accaggaaga agtagtcgcc caggccggac ttggtggggc ggctttcgac
     5341 cttggtcatc tggatattgg cctcggccag ctcggccagc acctcataga gcgcgcccgg
     5401 tacgttcgct ttgacccgga agcagaagga ggtcaggtcg ttgccggtcg gggccgcgtc
     5461 ctcgcgcccg agcacgaaga agcgggtcac gttgccgcga ttgtcctgga tatcgcgcgc
     5521 caggatctcg cccccgaaga gctcggccgc tcgcgtggtc ccgatggccg cgcggctgcg
     5581 gtctcccccg cgcacgacct cctccaccgc ggcggccgtg ctcagcgccg ccacctgagt
     5641 tacgttcggc aggcagcggt cgaggaagcg ccgcacctgt cccggcacct ggggatgggt
     5701 cagcacgacc gagatgtcgc gcagctcggt gcccggcacg gctaccaggt tgtggcgcac
     5761 ggccaccacc agctcggcca ggatcttgag actggtctcg tggatcaaga ggtcgagcgt
     5821 ggcgctgacc gagccctcca gcgagttctc gatcggcagg atggcgcgtt cggccagccc
     5881 ggtctcgacg gccgagacca gcgccgggat cgaggcaaag ggcatcggct cggagccgtc
     5941 gcggcgcgac tgcgccagcg cggcctcctc gctaaaggtg cccatcggac cgagataagc
     6001 aatgcgcatc gttccctccg gcggccaatc atagggggca gcacagcgcc ggtcaacgcg
     6061 gaggcgaccg gcggccaggc cgcgccggat cgagcagcgc tacggggcgt ggtattgtag
     6121 gaaatatctc ctcgcgctcc gagcggagcg gccgacccgg ccacctgacg acaggttcaa
     6181 gcgcgatgat tagcgactca gagatcgtcc tgcgactcgc catcagcgcc gccctgagcg
     6241 gcgtcgtggg gctggagcgc gagcggctgg agaagagcgc cggtctccgc acgcacgcgc
     6301 tggtcggcgt cggcgccacc ctggtcatga tcgtctccgc cttcggcttc gccgatgtcc
     6361 tcggcacgcc ggacgtcgta ctcgacccct cgcgcatggc ggcccaggtc gtcagcggga
     6421 tcggcttctt gggggcgggc acgatcatct tccgtcgcca gatcgtgcaa gggctgacca
     6481 ccgcggccag catctgggcg gtggcggcca tcgggttggc ggtcggcggc ggcatgtacc
     6541 tggccgcggc ggccgccacc gtgctcgccc tcttgatcct ggccggcatc aagccgatcg
     6601 agcatcgcat cttcacccag cgccagccgc cgctgctgac gctgctggtc gaccggcgcc
     6661 cgggcacgct ccccaccatc gaggccgtca tccagtcggc gggggttggg atcgagcaga
     6721 tcgtgatcga gcccggcgcc gaggaccacg aggaccgcgt cgagctgacc ctgacccagc
     6781 cgcactccgg cgcggcgctc ataccactgc tggagaccct gcgtcacatc cccggcgtgc
     6841 gcgagatccg cagcgcgagc agtacgccgc ccggctagca tgcgacgcac gacatcggct
     6901 gttgccgcac acgccacaag tcgttgtcga cggccacgcc cgccccagcg cccgaacacg
     6961 gcacttcgcc atcggcatac tatttgcact tcattcgcca tattcgcgtg ccgaccggtg
     7021 caccggtggc ctagcctgag aggacgatcg atggcccaca tccccaagga cgacattcag
     7081 cagctctgga acgagttgcc cagcaagaac tggtctgggc tgcacaaggt gctccagaac
     7141 caccagggca aggcgaacgg catctccaac aatctcgtcg acgacatgct gcgcatgagc
     7201 cagcgcaacg agcagtcggg caaggcgttc cccggctcga tggatcagct ctatcagcag
     7261 ttgaacagcg aactgcagag ctaacccccc ctctgatcca cgcccacacc ccgcgccggc
     7321 agccggcgcg gggtgcttat acccctcagt ttggccgcaa cgctggacgc cgccgcagtg
     7381 cgacggtatg ctgcggggtg gcatgggcat gggcgcggcc cgagcgctcg cgggcgccgt
     7441 cgcggttggc cgcggcgctg acgccgcggt gagccagggc agcagcggat gtgccgg
//
LOCUS       DASUBI010000725         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_946015, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000725 DASUBI010000000
VERSION     DASUBI010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946015"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFU72_14145"
     CDS             <1..824
                     /locus_tag="VFU72_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424681.1"
                     /translation="GDTALAWSLVGAWLADGPETPPGNTIYTCAIVLQRLAAALALDA
                     GDLPTAQAWLEAHDHWLAWNGTVLGQVEGALGWAEYHRAAGDLAAAREHADRALAHAT
                     EPRQPLALLAAHRLLGELATEAGDHAATAANLAEALALADACAAPYERALTLLALAEL
                     HAATSEREAASAVLTEARALLEPLEARPALARADVVAARLATTRPAAVAPAGLSAREV
                     DVLRLAAQGLTSAQIAARLSLSRRTVEQHLRSIYNKLGVDNRAAAVAFAVEHGLR"
     gene            1005..1868
                     /locus_tag="VFU72_14150"
     CDS             1005..1868
                     /locus_tag="VFU72_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5424682.1"
                     /translation="MLPQTIPASRPSDPFTASARWQREAIAQRHAEASAYLEDVFATP
                     ALRDIAAHTLALLALTPGEAVLEVGCGTGVFLPLLARAVGPAGRVVGLDLAPTFVAAA
                     RARIAAQGCAGAVTVEEGDAYRLPFPDASFDAAHCERVLMHLDDPTAVLREMRRVVRP
                     GGRVLAVEPDWAGCRLDHPDGAALDLLRARSLGQRQPDAGLTLYRRLADAGLIGREVV
                     PHPVPLTDLATLRLYGFTLQPGAAALVAEGQLTRERAETALAYLDEASAAGTFFACVT
                     AFIGLGRVPAP"
     gene            1978..2409
                     /locus_tag="VFU72_14155"
     CDS             1978..2409
                     /locus_tag="VFU72_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5424683.1"
                     /translation="MTAEANKKLVRRYGHVWHAGNQHLVDELAAPDLVVYYPLFGREV
                     RGHAAFKQVLTGTHVAFPDVQLVFEELLAEGDAVVARWRATATSRGAFAGRPPTGQRV
                     EWTGMTLYRIAGGKVIEERGEEDALGLFRQLGALPVPGATG"
BASE COUNT          341 a          958 c          883 g          337 t
ORIGIN      
        1 agggcgacac cgctctcgct tggtcgctgg ttggtgcgtg gctggccgat ggcccggaga
       61 cgccgccagg aaacacgatc tacacctgcg ccatcgtcct ccagcgcctc gccgccgcgc
      121 tggcgctcga cgccggcgat ctgccaactg cacaggcctg gcttgaggcg cacgaccact
      181 ggctggcctg gaacgggacc gtgctgggcc aggtcgaggg cgcgctcggc tgggccgaat
      241 accaccgcgc ggcgggcgac ctcgccgccg cgcgcgagca cgcggaccga gcactcgcac
      301 acgccaccga gccacgccag ccgctggctc tgctggccgc ccaccgcctc ctcggcgaac
      361 tcgcgactga ggcgggcgac cacgccgcaa ctgccgccaa cctggccgag gccctggcgc
      421 tggccgacgc ctgcgcggcg ccgtatgagc gcgccctgac gctgctcgcg ctggccgaac
      481 tgcacgccgc caccagcgaa cgcgaggcgg ccagcgcggt gctgaccgag gcccgcgccc
      541 tgctggaacc gctggaagcc cgtccggccc tggctcgtgc cgacgtcgtg gcggcgcggc
      601 tcgcgaccac gcgccccgct gcggtggctc ccgccgggct ctctgcccgg gaggtcgacg
      661 tgctgcggct agcagcgcag gggctgacca gcgcccagat cgccgcgcgc ctcagcctct
      721 cgcgccgcac cgtcgagcag cacttgcgct cgatctacaa caaactgggc gtcgacaacc
      781 gcgccgcggc cgtcgccttc gccgtcgagc acggcctgcg ctagccgccc gcgcgctgcg
      841 ccgtgcgtaa aactacgcag cgcgggctgg ccgcacgcgg cgagtagttg cgtaattccg
      901 acgatgtgcg cgccgccccg agcgcctgga atagcggcac cggcgcacca ggggccacag
      961 ggcgtgcgtc cccgccgcgc caggccagca gcacggaggg cgccatgctc ccacagacca
     1021 tccccgccag caggccatcg gacccgttca ccgcgagcgc acgctggcag cgcgaggcca
     1081 tcgcccagcg acacgccgag gccagcgcct acctggagga cgtctttgcc acgccggcgc
     1141 tgcgcgacat cgccgcgcac accctggcgc tcctggccct cacacccggc gaggcggttc
     1201 tggaggtcgg ctgcggcacc ggggtcttcc tgcccctgct ggcccgggcc gtcggccccg
     1261 ccggccgggt cgtcggcctc gacctggcgc ccacattcgt cgcggcggca cgggcacgaa
     1321 tcgctgccca aggctgcgct ggcgctgtca ccgtcgaaga aggggacgcc taccggctgc
     1381 ccttccccga cgcgtccttc gacgccgccc actgcgagcg cgtgctgatg cacctggatg
     1441 atccgactgc cgtgctgcgg gagatgcggc gggtggtgcg ccccggcggg cgggtgctgg
     1501 cggtcgagcc cgactgggcc ggttgccgcc ttgaccatcc ggacggggcg gcgctggact
     1561 tgttgcgtgc tcgcagcctc ggccagcgcc agccggacgc cggcttaacc ctctaccgcc
     1621 gcctggccga cgccgggctg atcgggcgcg aggtggtgcc gcacccggtg ccgcttaccg
     1681 acctcgccac gctgcgcctg tacggcttca ccctgcaacc gggggccgca gcactagtgg
     1741 ccgaagggca gcttacccgg gagcgcgctg agacggcact ggcctacctt gacgaggcca
     1801 gcgctgccgg gacgtttttc gcctgcgtca ccgccttcat cggcctcggt cgcgtcccgg
     1861 cgccctgagg cgttggttat gccaccaacg gcgctcagtg tgcttcccgc gccaggttgc
     1921 tagaatcgcg gtcgagtggg gctgctcaat cgtggataac cacaggagaa cacgacgatg
     1981 accgccgaag cgaacaagaa actggtgcgc cgctacgggc acgtctggca tgccgggaac
     2041 cagcacctcg tggacgaact ggccgccccc gacctggtcg tctactaccc gctcttcggc
     2101 agggaagtgc gcggccacgc ggccttcaaa caggtactta ctgggaccca tgtcgctttc
     2161 ccagatgtgc agcttgtgtt cgaggagctg cttgccgaag gggacgcggt ggtcgcccgc
     2221 tggcgcgcga cggccaccag ccggggggca ttcgccgggc ggccaccgac cggccagcgg
     2281 gtcgagtgga cggggatgac gctgtaccgc atcgccggcg gcaaggtcat cgaggagcgc
     2341 ggcgaggagg acgccctcgg cctcttccgg caactgggcg ccctccccgt cccaggcgca
     2401 acggggtagg ccgggcggcg cacgtctgcg ctgcggcgga ggcgtgacca ggccctggcc
     2461 aggccccgtt acgctcccgg ggtcgcgccc tggtcacggg cgcgcagccc gtcgaccat
//
LOCUS       DASUBI010000726         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1014115, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000726 DASUBI010000000
VERSION     DASUBI010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014115"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFU72_14160"
     CDS             <1..359
                     /locus_tag="VFU72_14160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424684.1"
                     /translation="GFFDRYPNLKLIAPHAGATLPYLAGRLDKCWDKMPACRVNIGEP
                     PSSYLRRIFYDTVAYEQNAIDLCLAVGGPSQLLYGSDYPHNIGDIEGCLARTEALPSE
                     VVEDVKSRNAMRVFGL"
     gene            391..1362
                     /locus_tag="VFU72_14165"
     CDS             391..1362
                     /locus_tag="VFU72_14165"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU5424685.1"
                     /translation="MPARGHPREYEVTVERPPDFDAFWGAIMAEAAAVPLNPELELVP
                     ARSTDAVEVYEIHYDSLERLRVAGWYCRPREAALPPPYPGLLIVPGYISEPTLPKSWA
                     RLGYAAVGVAPRGKLRSNAHFNPGYPGLLVHNITDRHTYGYRGFYVDAARAVDFLLTR
                     PEVDPARIGVHGSSQGGALTVTTAALRRDVIACGAAGAPYLCGFLDAAALTHSYPYEE
                     INEYLRQHPEHEPLVRETLAYYDILNFASEIQAPMLVYLGLEDDVCPPETGFALFEAL
                     RCPKELYAYEHCAHDAGSYWAMGRVERFLARHLQPAGAAVGAETTVG"
     gene            1381..2292
                     /locus_tag="VFU72_14170"
     CDS             1381..2292
                     /locus_tag="VFU72_14170"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU5424686.1"
                     /translation="MTIASDSQRPADFAEFWESVDRELARYPARPALEPLPRHGTDFC
                     DVYALRLTSLGPYRIFGYYSVPRGPGPFPALLTTPRYGSVNHVPDYNDRQRYVCLTLM
                     HRGQRLADEPYAAAYPGLLTEGIADPQTYIYRGIVADCLRGAEFLRERPEVDQSRVAV
                     AGDDLALLAAARRPGFAVVQAAGLLLYRLSEAAGRGTAYPVEEINDYLRPHPGQTDAV
                     ARTLAYFEPRYHAPAVTATTILSLDDEGTLGGLPWLQPLREALGGPVVPYRLCHEGGT
                     DHDQLDALLAQQLDVPPLSRFGGEWPS"
     gene            2289..3419
                     /locus_tag="VFU72_14175"
     CDS             2289..3419
                     /locus_tag="VFU72_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5424687.1"
                     /translation="MSLYQELGVRRVINASATLTRLGGSLMPPPVLAAMQEAAGWFID
                     LPELQRRVGARIAELTHNDACYVSSGAAAGITLAVAACLTGDDPRLIDAMPYLEEAPQ
                     TEVVVFRGQRNGYDYAARQTGARLIEIGPDAAELSDALNARTACVLYFAGAHFAAGAL
                     PLEAVVPLAHARGVPVLVDAAAQIPPISNLWRFTRELGADAAIFSGGKGLRGPQSSGL
                     VLGRREIVEGCRRNGPPNHSIGRPMKVGKEELAGLLAAVEWSLAQDEAAVIAGYERSV
                     ARWVEGLAGIPGVTAARGFPSEAGQPHARAIVRLSPPCRLGRDDVVAALWERDPRVAV
                     GTTGEDAIALNPQTLAPGEDELVLAALRAVLTGGSAAVNAAE"
     gene            3620..>4042
                     /locus_tag="VFU72_14180"
     CDS             3620..>4042
                     /locus_tag="VFU72_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5424688.1"
                     /translation="MEQDQRLVGQRYGLIRRVGSGGMADVYLAEDRRLGRRVAVKVLH
                     PAEARDPAAVARFKREAMAAAALDHRGIVPIYDWGESDDTYYIVMAYVPGEDLKAALA
                     RRGALPEAEALGIARQVAAALATAHAHGIVHRDIKPHNI"
BASE COUNT          574 a         1452 c         1432 g          584 t
ORIGIN      
        1 acggcttctt cgatcgctac ccgaacctga aactgatcgc gccgcacgcg ggggccacgc
       61 tcccctacct cgccgggcgc ctggataagt gctgggataa gatgcccgcc tgccgggtca
      121 atatcggcga gccgccctcc agctatctgc ggcgcatctt ctacgacacg gtggcctacg
      181 agcagaacgc catcgatctc tgcctggcgg tgggcggtcc cagccagctc ctctacggct
      241 ccgactaccc acacaacatc ggcgacatcg agggctgcct ggcgcgcacc gaggcgctgc
      301 cgagtgaggt cgtcgaggac gtcaagagcc gcaacgccat gcgggtgttc gggttgtagg
      361 ggagggcccg taccagaaag gcgacaggtc atgccagcgc gtgggcaccc gcgggagtac
      421 gaggtcaccg tcgagcggcc gcctgacttc gacgccttct ggggcgcgat catggccgag
      481 gcggctgccg ttccgctcaa tccggagctg gagctggttc cggcgcgctc gaccgacgcg
      541 gtcgaggtct acgagatcca ctacgatagc ctggagcgcc tgcgcgtggc gggctggtac
      601 tgccggcccc gcgaggccgc gctgccgccg ccctatccgg ggctgctgat tgtgcccggc
      661 tacatctccg agccgacgct gcccaagtcg tgggcgcggc tcggctacgc cgccgtcggg
      721 gtggcgccgc gcggtaagct gcgctccaac gcgcacttca accccggcta ccccgggctc
      781 ctggtgcaca acatcaccga ccgccacacc tatgggtatc ggggcttcta cgtcgacgcg
      841 gcgcgggccg tcgatttcct gctgacccgg ccggaggtcg atccggcacg catcggcgta
      901 cacggcagca gccagggcgg cgcgctgacg gtcaccaccg ctgccctgcg gcgcgacgtg
      961 atcgcctgcg gagcggccgg cgcaccgtat ctgtgcggct tcctggacgc cgccgcgctg
     1021 acgcattcct acccctacga ggagatcaac gagtatctgc gccagcaccc ggagcacgag
     1081 ccgctggtgc gcgagacgct cgcctactac gacatcctca acttcgcctc ggagattcag
     1141 gcgccgatgc tggtctacct gggactggag gacgacgtct gtccgccgga gaccggcttc
     1201 gcgctcttcg aggcgctgcg ctgccccaaa gagctgtatg cctacgagca ttgcgcccac
     1261 gatgccggca gctattgggc catgggccgg gtagagcgct tcctggcgcg gcatctccaa
     1321 ccggccggcg cggcggtcgg cgccgagacg acggtcggct aggcgcgagc gggagggatg
     1381 atgacgatcg catccgacag ccagcggccc gccgacttcg ccgagttctg ggaatcggtc
     1441 gaccgggagc tggcgcgcta tccggcgcga ccggcgctgg agccgctgcc gcgccacggc
     1501 accgacttct gcgacgtcta cgccctgcgg ctgaccagcc tcggccccta tcgcatcttc
     1561 ggctactaca gcgtgccgag aggtcccggc cccttcccgg cgctgctgac cacgccgcgc
     1621 tacggcagcg tcaaccacgt gcccgactac aacgaccggc agcgctacgt ctgcctgacg
     1681 ctgatgcacc gtggtcaacg actggccgac gagccctacg cggcggccta ccccggcctg
     1741 ctgaccgagg ggatcgccga cccgcagacg tacatctacc gtggcatcgt ggccgactgc
     1801 ctgcgcggtg ccgagttcct gcgtgagcga cccgaggtcg accaaagccg cgtggcggtc
     1861 gccggggacg atctggcgct gctcgcggcg gcacgccgtc ccggtttcgc cgtcgtgcag
     1921 gcggccgggc tgctgctcta tcgtctgtcc gaagcggccg ggcgcggcac ggcctacccg
     1981 gtcgaggaga tcaacgacta cctgcgcccc cacccgggcc agaccgacgc ggtggcgcgc
     2041 acgctggcct acttcgagcc gcgctaccac gcgccggccg tcacggccac caccatcctc
     2101 agcctggacg acgaggggac gctgggcggg cttccctggc tgcaaccgct acgcgaggcg
     2161 ctgggcggcc cggtcgtgcc ctaccgcctc tgccacgagg gcggcaccga ccacgaccag
     2221 ctcgacgcgc tgctggcgca gcaactcgac gtgccgccgc tgtcgcgctt cgggggggag
     2281 tggccgtcgt gagcctctac caggagctcg gtgtccgccg cgtgatcaac gcctcggcca
     2341 cactgacacg gctgggcggc tcgctgatgc cgccgccggt gctggcggcg atgcaggagg
     2401 cggccggctg gttcatcgac ctgcccgagc tgcagcgacg ggtgggcgcg cgcatcgcgg
     2461 agctgaccca caacgacgcc tgctacgtct cctccggcgc ggcggctggc atcacgctgg
     2521 cggtggccgc ctgcctgacg ggcgacgatc cgcgcctgat cgatgccatg ccctacctgg
     2581 aggaagcgcc ccagaccgag gtcgtcgtct ttcgcggcca gcgcaacggc tacgactacg
     2641 cggccaggca gaccggcgcc cggctgatcg agattggtcc ggatgccgct gagttgtccg
     2701 acgcgctcaa cgcccgcacc gcctgcgtgc tctacttcgc cggggcgcac ttcgccgcgg
     2761 gggcgctgcc gctggaggcg gtcgtgccgc tggcgcacgc gcggggcgta ccggtgctgg
     2821 tcgacgcggc ggcgcagatc ccgccgatca gcaacctctg gcgcttcacg cgtgagctgg
     2881 gcgccgacgc cgccatcttc agcggcggca aggggctgcg cgggccgcag tcgagcgggc
     2941 tagtcttggg gcggcgcgag atcgtcgagg gctgccggcg caacggtccg cccaaccaca
     3001 gcatcgggcg cccgatgaag gtggggaaag aagagctggc gggactgttg gcggcggtcg
     3061 agtggtcgct ggcgcaagac gaggcggcgg tcatcgccgg ctacgagcgc tcggtggcgc
     3121 gctgggtgga ggggctggcc ggcatccccg gcgtgacggc cgcgcgtggc ttccccagcg
     3181 aggcgggcca gccgcacgcc cgcgccatcg tgcgtctgtc gcccccttgc cggctcggcc
     3241 gcgacgacgt ggtggcggcc ctctgggagc gcgatccgcg cgtcgcggtc ggcaccaccg
     3301 gcgaggacgc catcgcgctc aacccccaga cgctcgcccc cggcgaggac gaactggtcc
     3361 tggccgccct gcgcgccgtg ctgaccggcg gtagcgcagc tgtgaacgcc gcggaatgag
     3421 cgctgggccg ccgacctgat ccggtttacg gcagcctccg cgtagccgat gctcaggcgg
     3481 gcgtagccct cagacagacg gtgtccggca cggttcattg gaactgcgcc gctggcatcc
     3541 cgcttgccgc taccagcccc atctatgcgc ctgctagcat tatggtgacg aagtcgtcat
     3601 ccacggatgg gcctgaagca tggagcagga tcagcggctg gtcgggcagc gctacgggtt
     3661 gattcgccgg gtgggcagcg gcgggatggc cgatgtctac ctggcggagg accggcgcct
     3721 gggccggcgc gtcgcggtca aagtgctcca ccccgccgag gcgcgcgacc ccgcggcggt
     3781 ggcgcgcttc aagcgcgagg cgatggcggc ggccgcgctc gaccatcgcg gcatcgtgcc
     3841 gatctacgac tggggcgaga gcgacgacac ctactacatc gtgatggcct acgtccctgg
     3901 cgaggacctg aaggcggcgc tggcgcggcg tggcgcgctg ccggaggccg aggcattggg
     3961 catcgcgcgg caagtggccg ccgcgctggc cacggcgcac gcccacggca tcgtgcaccg
     4021 cgacatcaag ccgcacaaca tc
//
LOCUS       DASUBI010000727         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_355547, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000727 DASUBI010000000
VERSION     DASUBI010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355547"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="hemG"
                     /locus_tag="VFU72_14185"
     CDS             <1..921
                     /gene="hemG"
                     /locus_tag="VFU72_14185"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU5424689.1"
                     /translation="IYAGDAEQLSILATFPRLRETELRHGSLIRGMLAQRRAAPPTAP
                     AGPNRSGFLSLQGGLEELIAALAARLDGCENVALRTSTPVAGVAARPEGGYALALGNG
                     ERLETDALLVAAPAGPAADLLAGFDPELAAPLAEIPYVSSATVALAYPREVVASLGGG
                     RGFVVPRVEGRELTAVTWVSSKFAGRAPEGMALVRGFVGRAGHEDAVRLPDDALIALV
                     RRELRDILGLTAEPARAWVARWERALPQYVLGHQERLARIEARVAAHPGLALCGAAYR
                     GVGIPDCIQSGETAAEHVAAALAVGTPHVV"
     gene            complement(1172..1651)
                     /locus_tag="VFU72_14190"
     CDS             complement(1172..1651)
                     /locus_tag="VFU72_14190"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEU5424690.1"
                     /translation="MRRLLLLLGLILTAGWLLAACGQGASAAKPPEIHYGVDVAECGM
                     IISDARFAASQVDARGQAVLYDDAGELALAAQQHGPGTDHLWVHDYDSKDWLDATAAF
                     YVSSEEFRPQTPMGSGVVAFKTRQQGEVFAAGHQGTVMTWDEMMTGWHDPGMGMHHG"
     gene            complement(1651..2481)
                     /locus_tag="VFU72_14195"
     CDS             complement(1651..2481)
                     /locus_tag="VFU72_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424691.1"
                     /translation="MERSIVVAIAAREARGGLRNRWFLLYAAVFGVLIVAFSWIALSS
                     SDVVGQAGFARTSAGLLNLILLMVPLIGLTVGAQSLASERQDRSLDYLMAQPVSAAEI
                     FTGKYLGAALSLLLMLLLSFGWAGLTLAARGSATGLHDFLLLIALTLLLALGMLSLGY
                     LISSAARQTATALGIALTAWLGLIVIGDLGLLGSALVMNLQPATLLFATMSNPLDAYK
                     LLNVEVLHTSLEVLGPAGSYAIDRFGGRLPAVLLAIEALWVVLPLPIGYRLFKHTEVR
                     "
     gene            complement(2463..>4769)
                     /gene="nosD"
                     /locus_tag="VFU72_14200"
     CDS             complement(2463..>4769)
                     /gene="nosD"
                     /locus_tag="VFU72_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEU5424692.1"
                     /translation="LAPPLVLAADPPALTVCPTCAVRSIGDALAQAAPGARIVVDGGR
                     YPGGLVIDKPVQLSGENGAVIDAGEQGTAVTITAPGVTVENLTIRGTGISLDHEDAAV
                     MVKAERATIAGNRIEDALFGIYLKQGHDSVIRDNVVESKALPVDQRGDAIRAWYSNNV
                     QVERNVARNGRDIILWFSNDGIVRDNTFEGNRYGLHLMYSDRAQIEGNALRANSVGIY
                     AMYSRDFTVRGNAITDSHGASGMGLGLKTVDNATISDNRIVSNRIGMQVDDSPSDADA
                     RLTVSGNVFAYNGSGIAFLPNAQRDFVVGNDFIDNSEQIAVLGGGTPRGITWSQDGRG
                     NYWSDYAGYDANGDGIGDRPYQARQLFQSLVDRHDSLRIFLYSPVVTAIDFAARAFPE
                     VQPESKLVDSAPLMRPVVPAGLPTAAASQSNRLFMGAVGFLAVIGGFLMIALLRRPRT
                     KPAAPVAPALPSAPRPLAPLPGEPTALAMIQVRQLTKRYGAMTAVDDAAFDVARGHSL
                     ALWGPNGAGKTTILRCLLGTAGYSGHIAIDGLDPRRDGRLARQRIGFVPQELPVTPMT
                     VLEMLQFIAALKHVPLAEAQERLQLLGIADQAAKPVAALSGGQRQRLALALALIGDPA
                     ILLLDEPTANLDARGRAELLELLRELKQQGKTLVFASHRQEDVLMLADQVLMIVDGVV
                     RELLTPAEFLRQVEADARLVLYLEHGDVPVAVDELDRLGCPATASGRVVSIATRGQAT
                     GHILGELARAGVVVNEFALERGTWNARS"
BASE COUNT          696 a         1637 c         1751 g          685 t
ORIGIN      
        1 atctacgccg gcgacgccga gcaactgagc atcctggcca ccttcccgcg cctgcgcgag
       61 accgagctgc gccacggcag cctgatccgc ggcatgctgg cccagcgccg cgcggccccg
      121 ccgacagcgc ccgccggacc aaatcgctcc ggcttcctca gcctgcaagg gggcctggag
      181 gagctgatcg cggcgctggc cgcccggctc gacggctgcg agaacgtcgc gctgcgcacc
      241 agcacgccgg tggcgggtgt ggcggcgcgg ccggagggcg gttacgcgct cgcgctgggg
      301 aacggcgagc ggctggagac tgacgcgctg ctcgtggccg cgccggccgg ccccgccgct
      361 gacctgctgg ccgggttcga cccggagctg gcggcgccgc tggccgagat cccctacgtc
      421 tcctcggcca cggtggcgct ggcctatccg cgagaggtgg tcgccagcct cggcggcggc
      481 cggggcttcg tggtgccgcg ggtcgagggg cgcgagctga ccgccgtcac ctgggtcagc
      541 agcaagttcg ccgggcgcgc gccggagggg atggcgctgg tgcgcggctt cgtcggccgc
      601 gctgggcatg aggacgcggt gcgcctgccc gacgacgcgc tgatcgcgct ggtgcggcgc
      661 gagctgcgcg acatcctggg gctaaccgcc gagccggcgc gggcctgggt cgcgcgttgg
      721 gagcgggcgc tgccgcagta cgtgctgggc caccaggagc ggctggcgcg cattgaggcg
      781 cgtgtggcgg cgcacccggg gctggcgctc tgcggcgccg cctatcgcgg cgtcggcatc
      841 cccgattgca tccagagcgg cgagaccgcc gcggaacatg tcgcggccgc gctcgccgtg
      901 ggcacgccgc acgtggtcta aacgcggtgc cctcccgacg gcgcacgtgt gaggctacga
      961 cggatccttc gacaagctca ggatgacagg gctaagaagg ggcggacgac gacgactgac
     1021 gtagtcgtta gccccatccc tcaccctctt ggagggtcgc aaatcactga tgttggtaac
     1081 cctgaccgac cagcccgtga caggcgagcg cccgcggcac ggcccctggc cgtcgcagcg
     1141 ggcgctcgct cccccgttcc cgcacggcac cctacccgtg atgcatgccc atccccgggt
     1201 cgtgccaacc ggtcatcatc tcgtcccagg tcatcaccgt gccctggtgg ccggcggcga
     1261 agacctcgcc ctgctggcgc gtcttgaagg cgaccacgcc cgagcccatc ggcgtctgcg
     1321 gccggaactc ctcactggag acgtagaagg cggcggtggc gtcgagccag tccttgctgt
     1381 cgtagtcgtg gacccagaga tggtccgtgc ccggcccgtg ctgctgcgcc gccagggcca
     1441 gctcgcccgc gtcgtcgtag agcacggcct gcccgcgagc gtcgacctgg ctggccgcga
     1501 agcgcgcgtc ggagatgatc atgccgcact cggccacatc gacgccgtag tgaatctccg
     1561 gcggtttggc ggcgctggcg ccctgtccgc aggccgcgag cagccagcct gcggtcagga
     1621 tcaggcccag gagaagcagc aggcggcgca tcagcgcacc tccgtgtgtt tgaagaggcg
     1681 atagccgatg gggaggggca gcacgaccca gagcgcctcg atcgcgagca gcacggccgg
     1741 caagcggccg ccgaagcgat cgatcgcata gctgccggcc ggaccgagga cctccagcga
     1801 ggtgtggagc acctcgacgt tgagcagctt gtaggcgtcg agcgggttgg acatggtcgc
     1861 gaacagcagc gtagcgggct ggagattcat gaccagggcg ctccccagga ggcccagatc
     1921 gccgatgacg atcagcccca gccaggcggt gagggcgatg ccgagcgccg tcgcggtctg
     1981 acgcgcggcg ctggagatga ggtagcccag gctgagcata ccgagcgcca gcagcagcgt
     2041 cagcgcgatc agcagcagaa agtcgtgcag cccggtggcg ctgccgcgcg cggccagcgt
     2101 gagccccgcc cagccgaagc tgagcagcag catcagcagc agcgagagtg ccgccccgag
     2161 gtatttgccg gtgaagatct cggcggcgga gaccggctgg gccatcaggt agtccagcga
     2221 gcggtcctga cgctccgaag ccaggctctg cgcgccgacg gtcagtccga tcagcggcac
     2281 catcagcagg atcaggttga gcagcccggc cgaggtgcgg gcgaagccgg cctggccgac
     2341 cacatccgag ctggagaggg cgatccagga gaaggccacg atgagcacgc cgaagacggc
     2401 ggcatagagc aggaaccagc ggttccgcag gccgccgcga gcctcgcggg cggcgatggc
     2461 gactacgatc gagcgttcca tgtgcctcgc tccagtgcga attcgttcac gaccacgccg
     2521 gcccgcgcca gctcaccgag gatgtggccc gtggcctggc cacgggtcgc gatcgagacc
     2581 acccgcccgc tggcggtggc cgggcagccg agccggtcga gctcgtccac cgcgacgggg
     2641 acgtcgccgt gctccaggta gagcaccagg cgcgcgtcgg cctcgacctg gcgcagaaat
     2701 tcggccgggg tgagcagctc gcgcacgacc ccgtcgacga tcatcagcac ctgatccgcc
     2761 agcatcagga cgtcttcctg ccggtgcgag gcgaagacga gcgtcttacc ctgctgcttc
     2821 agctcccgca agagctccag gagctcggcc cgcccccggg cgtcgagatt ggcggtcggc
     2881 tcgtcgagca gcaggatcgc cgggtcgccg atgagcgcca gcgccagtgc cagccgctgc
     2941 cgctgcccgc ccgagagtgc ggccaccggc ttggcggcct ggtcggcgat cccgagcagt
     3001 tggagccgct cctgggcctc agcgagcggc acgtgcttca gcgcggcgat gaactggagc
     3061 atctcgagca cggtcatcgg cgtcaccggc agctcctgcg gcacgaagcc gatgcgctgc
     3121 cgcgccaggc ggccatcccg ccgcgggtcg agcccgtcga tcgcgatgtg cccgctgtag
     3181 ccggcggtgc cgagcaggca gcgcaggatg gtggtcttgc cggcgccgtt cggcccccag
     3241 agggccaggc tgtgcccgcg cgccacgtcg aaggccgcgt cgtcgacggc ggtcatggcg
     3301 ccgtagcgct tggtcaactg acgcacctga atcatggcca gggcggtggg ctcgccgggt
     3361 agcggcgcca gcggacgcgg cgccgacggc aaggccggcg cgaccggagc ggccggcttc
     3421 gtgcgcggac gccggagtag cgcgatcatc aggaatcccc cgatcactgc caggaacccg
     3481 acggctccca tgaacaggcg gttcgattgg ctggccgcgg ccgtgggcag gcccgcgggc
     3541 acgaccggcc gcatcagcgg cgcggagtcg acgagtttcg actcgggctg gacctcggga
     3601 aaggcccggg ccgcgaagtc gatggcggtc accaccgggc tgtagaggaa gatccgcagc
     3661 gagtcgtggc ggtcgaccag ggattgaaac aactggcgcg cctggtaggg ccggtcgccg
     3721 atgccgtcgc cgttggcgtc gtagccggcg tagtcgctcc agtagttgcc gcggccgtcc
     3781 tggctccagg tgatcccgcg cggggttcct ccgccgagga cggcgatctg ctcgctgttg
     3841 tcgatgaagt cgttgccgac cacgaagtcg cgctgggcgt tggggaggaa ggcgatgccg
     3901 ctgccgttgt aggcgaacac gttgccgctg acggtcagcc gtgcgtcggc atccgagggc
     3961 gagtcgtcga cctgcatccc gatgcggttg ctgacgatgc gattgtcgct gatggtcgcg
     4021 ttgtcgacgg tcttgagtcc cagccccata ccgctggcgc cgtggctatc ggtgatcgcg
     4081 ttgccgcgca ccgtgaagtc gcggctgtac atggcgtaga tcccgaccga gttcgcccgc
     4141 agcgcgttgc cttcgatctg ggcgcggtcg ctgtacatca ggtggagccc gtagcggttc
     4201 ccctcgaagg tgttgtcgcg gacgatgccg tcgttcgaga accagagaat gatgtcgcgt
     4261 ccgttgcgcg ccacgttgcg ctcgacctgc acgttgttgc tgtaccaggc gcggatggca
     4321 tcgccgcgct ggtcgacggg cagcgccttg ctctccacca cgttgtcgcg aatgacactg
     4381 tcgtggccct gtttgagata gatgccgaag agcgcgtcct cgatgcggtt gccggcgatg
     4441 gtggcacgtt cggccttgac catgaccgcg gcatcctcgt ggtcgaggct gatgccggtg
     4501 ccgcgaatgg tcaggttctc gacggtgacg cccggcgccg tgatcgtcac ggccgtaccc
     4561 tgctcgcccg cgtcgatgac cgcgccgttc tcgccgctga gctgcaccgg cttgtcgatg
     4621 accaggccac ccgggtagcg cccgccgtcg accacgatgc gggcgccggg cgcggcctgc
     4681 gccagcgcgt cgccgatcga ccgtaccgca caggtcgggc agacggtgag cgcgggcggg
     4741 tccgcggcca gcaccagcgg cggcgccag
//
LOCUS       DASUBI010000728         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_565004, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000728 DASUBI010000000
VERSION     DASUBI010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565004"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFU72_14205"
     CDS             complement(<1..658)
                     /locus_tag="VFU72_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5424693.1"
                     /translation="MAESNVLVDLSRQLADVVEQAQPWIVRVEARRRVGATGIVWSAD
                     GLIVTADHVVERDEEITVGLADGRELPARLVGRDQSVDLAVLKVEASGLPAAQPAQQP
                     AKTGNVVLALGRPFGGNAMASLGIVSAVGGQWRSWRGGSIEGIVRSDVTLYPGFSGGP
                     LVDGDGRLIGVNSSILARGLAVAIPHAAIGKTVAALTSPGGAKRGYLGIASQPVKIPE
                     G"
     gene            complement(1057..2028)
                     /locus_tag="VFU72_14210"
     CDS             complement(1057..2028)
                     /locus_tag="VFU72_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424694.1"
                     /translation="MSQATGSVDTTFTQEGTRAARATSLWGDAFRRFTRNRLAAASAI
                     VILALAVLAILAPLISPDSYWHQNYSAINAGPSLSHPFGTDQLGRDLFSRLLYGARIS
                     LAVGIVVQFVIVLIGVPVGLAAGFYGGKIDTALMRIVDVMYAMPSLLFVIIIMTFLRG
                     VLDQPHGGGFMRPIAQLDGRLGGLFGVFIGLGLISWLTVARIVRAQALSLKEKEFVEA
                     ANGMGASNRRIMFRHILPNTLAPIIVAVTLGIPRAILYEAGISFLGLGVTPPMPSWGL
                     MISEAIPTLRSYPYMLVFPAIALSLTVLAFNFLGDGLRDALDPWSLR"
     gene            complement(2051..2983)
                     /locus_tag="VFU72_14215"
     CDS             complement(2051..2983)
                     /locus_tag="VFU72_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424695.1"
                     /translation="MKRRGYIIRRVLLLFPTILAIYTITFVLMHATPGGPWSNTGDKP
                     IPPIVLQRLNQAYGLDKPLWQQYVNYLDHVLHGDFGPSYAQRSLTVSDIIKQAFPVSF
                     KLGLIAMAIAIVSGVSLGVLGAIKHNTWVDYIASFAAIVGISTPSYVVVSLLVLILAS
                     HLHLVPTGGWKGAFSSLVIIPAIALALYPAAVLARYTRSSMLDVLKMDYIRTARAKGL
                     RERRVIVRHAIQNSLLPVVTVAGLILADVITGSFFVESIYSVPGLGRYFVQSITNRDY
                     PVILGTVLLFGVVISVMNLIVDLLYQVLDPRIQT"
     gene            complement(3135..>3700)
                     /locus_tag="VFU72_14220"
     CDS             complement(3135..>3700)
                     /locus_tag="VFU72_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424696.1"
                     /translation="ANIGEDVAKAKQLLTDAGVSDPASLDLTLVYISTADYKLDAEYV
                     QQAWKNNLGLNIKLEPVESTAYNDWRASREKSPFNLYIGNWGSDWADPANWYNQNFTH
                     TSDHYRNHWNDPEFDALVEKARTNTNEDERVSQYKQASKMFAEESPIIPLYHPLNFWV
                     VQPYVKDLAMQPLLETVRARYIKIAKH"
BASE COUNT          619 a         1195 c         1296 g          590 t
ORIGIN      
        1 gcccctcggg gatcttgacc ggctgcgagg cgatgccgag gtagccacgc ttggccccgc
       61 cggggctggt cagcgccgcg acggtcttgc cgatggcggc gtgcgggatg gccacggcca
      121 ggccgcgcgc caggatcgag ctgttgaccc cgatcagccg gccgtcgccg tcgaccagcg
      181 gtccaccgga gaagccgggg tagagcgtga cgtcgctgcg cacgatcccc tcgatgctgc
      241 cgccgcgcca ggagcgccac tgcccgccga ccgcgctgac gatgccgagc gaggccatgg
      301 cgttgccgcc gaagggacgc ccgagcgcca ataccacatt gccggtcttg gccggctgtt
      361 gtgccggctg cgcggccggc agcccgctcg cctcgacctt cagcacggcc agatcgacgc
      421 tttgatcgcg cccgaccagc cgggccggca gctcgcgccc gtcggccagc ccgacggtga
      481 tctcctcgtc gcgctcgacc acgtggtcgg cggtcacgat caggccgtcg gcggaccaga
      541 cgatgccggt cgcgccgacg cgccgccgcg cttcgacgcg cacgatccag ggctgcgcct
      601 gctccaccac gtccgccaac tgccgcgaca ggtcaaccaa cacgttgctc tccgccatgg
      661 agtcctccta gtgcgttgtc ttcgaccagc ccgccccgcc ggacgcgtca gcggacccac
      721 tccgagcata ggcccgccgc ccccggccga caccgcccgt tccaacggcg cgcaccctcg
      781 acaaaaggct aggtcggggg gaggggattg cccatcgcgt gcgtatcgcg gcgtgtgcgg
      841 cttggatcct tcgacaagct caggatgaca gggtagggga tgagacctgc cggacagaca
      901 ggtcgtcgcc caagacccat tctcctggtc agctccgtac tctccctgtc atcccgagcg
      961 gagtcgaggg atccgtcctc aaatggcact caacgcattc ggcggccacc tcccctcgcc
     1021 cacgcatggg agaggggctg ggggtgaggg cccgtcctag cgcagcgacc aggggtcgag
     1081 ggcgtcgcgc aggccgtcgc cgaggaagtt gaaggcgagc acggtcagcg agagcgcgat
     1141 ggccgggaag acgagcatgt aggggtagga gcgcagcgtc gggatcgcct cgctgatcat
     1201 caggccccag ctcggcatcg gcggcgttac ccccagaccc aggaagctga tgcccgcttc
     1261 gtagaggatg gcgcgcggga tgcccagcgt gaccgccacg atgatcggcg cgagggtatt
     1321 cggcaggatg tgccggaaca tgatgcgccg gttcgaggcg cccatcccgt tggccgcctc
     1381 cacgaactcc ttctccttga gcgagagcgc ctgcgcgcgg acgatgcgcg ccacggttag
     1441 ccagctgatc agccccaggc cgataaagac gccgaagagg ccgcccagtc gcccgtcgag
     1501 ctgggcgatc gggcgcatga agccgccgcc gtgcggctga tcgagcacgc cgcgcaggaa
     1561 ggtcatgatg atgatcacga acagcaggct gggcatggcg tacatcacgt cgacgatgcg
     1621 catcagggcc gtgtcgatct tgccgccgta gaagcccgcc gccagcccga ccggcacgcc
     1681 aatcagcacg atcacgaact gcaccacgat gccgaccgcc agcgagatgc gcgcgccata
     1741 gaggaggcgg ctgaaaaggt cgcgcccgag ctggtcggtg ccgaaggggt gcgacaggct
     1801 cggcccggcg ttgatggcgc tgtagttctg gtgccagtag ctgtcggggc tgatcagcgg
     1861 cgccaggatg gcgagcacgg ccagcgccag gatgacgatg gccgaggccg ccgccagccg
     1921 gttgcgcgtg aagcggcgga aggcgtcgcc ccagaggctg gtggcgcgag ccgcgcgggt
     1981 tccctcctgg gtgaacgtgg tatcgaccga ccctgtcgcc tggctcatgc gctgcgctcc
     2041 aacctggtcg ctatgtctga atgcggggat cgaggacctg atagagcaga tcgacgatca
     2101 ggttcatgac cgagatgacg accccgaaga gaaggaccgt acccaggatg accgggtagt
     2161 cgcggttggt gatgctctgg acgaaatagc gtcccagccc cggcaccgag tagatcgact
     2221 cgacgaagaa ggagccggtg atgacgtcgg ccaggatcaa cccggccacg gtgaccaccg
     2281 gcaggagcga gttctggatg gcgtggcgca cgatcacccg gcgctcgcgc agccccttgg
     2341 cgcgcgccgt ccgaatgtag tccatcttga gcacgtcgag catgctggag cgcgtgtagc
     2401 gcgccagcac cgccgccggg tagagcgcca gggcgatggc cgggatgatc accagggagc
     2461 tgaaggcgcc cttccagccg ccggtcggca ccaggtgcag gtggctggcc aggatcagca
     2521 ccagcagcga gacgaccacg tacgaagggg tcgagatgcc gacgatggcg gcgaagctgg
     2581 cgatgtagtc cacccaggtg ttgtgcttga tcgcccccag cacaccgagc gacaccccgg
     2641 agacgatggc gatcgccatg gcgatcaacc cgagcttgaa cgagaccggg aaggcctgct
     2701 tgatgatgtc ggagacggtc agcgagcgct gcgcgtagga ggggccgaaa tcaccgtgca
     2761 gcacgtggtc gaggtagttg acgtactgct gccagagtgg cttgtcgagc ccgtaggcct
     2821 gattgagccg ctgcagcacg atcggcggga tcggcttgtc gcccgtgttc gaccaggggc
     2881 cgcccggcgt ggcgtgcatc aggacgaagg tgatggtgta gatcgccagg atggtgggga
     2941 agaggagcag aacccggcga atgatgtagc cccgtcgttt catgtcaggt ttgtgccttt
     3001 ttcacgtctc cccttggtgc cacgccgttg ggggagaggg cggccacggg gggccgcccc
     3061 tacaggaaac acgcgcgttc ctcaacctgt aggggcagac ccccgtgtct gccctcccac
     3121 gcgcggcgtc accgctagtg cttggcgatc ttgatgtagc gcgcgcgcac ggtctccagc
     3181 agcggctgca tagccaggtc tttgacgtag ggctgcacca cccagaagtt cagcgggtgg
     3241 tagagcggga tgatcggcga ctcctcggcg aacatcttgc tggcctgctt gtactgcgac
     3301 acgcgctcgt cctcgttggt gttggtgcgc gctttctcca ccagcgcgtc gaactccggg
     3361 tcgttccagt ggttgcgata gtgatcgctc gtgtgggtga agttctggtt gtaccagttg
     3421 gccgggtcgg cccagtcgga gccccagttg ccgatgtaga ggttgaaggg cgacttctcg
     3481 cgcgaggcgc gccagtcgtt gtaggcggtg ctctcgaccg gctccagctt gatattgagc
     3541 cccagattat tcttccaggc ctgctgcacg tactcggcgt cgagcttgta gtcggcggtg
     3601 ctgatgtaga ccagcgtgag atcgagcgag gccgggtcgc tgacgccggc gtcggtcagc
     3661 agttgcttgg ccttggcgac gtcctcgccg atgttggcgt
//
LOCUS       DASUBI010000729         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_3981, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000729 DASUBI010000000
VERSION     DASUBI010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..3745
                     /locus_tag="VFU72_14225"
     CDS             350..3745
                     /locus_tag="VFU72_14225"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU5424697.1"
                     /translation="MKKRPSARGLRVARVFTSPGQDPFETISWARRTSRITNPDGSVV
                     FELADAEIPAPWSQVATDIMVSKYFRKAGVPQHDTAGNPLLDADGAPVLGAEQSARQV
                     IGRLAGTWRDWGERYGYFAGEADAAAFEDELKYMLVHQMAAPNSPQWFNTGLHWAYGI
                     TGPAQGHAYVDPESGELRHSEDAYSRPTPHACFIQSVEDDLINEGGIFDLVTREARVF
                     KYGSGSGTNFSKIRAEGEQLSGGGTSSGLMSFLKIFDRAAGAIKSGGTTRRAAKMVVV
                     DIDHPDIEKFISWKAEEEKKVAALIAAGYSNDFNGEAYATVSGQNSNNSVRVTDDFIK
                     AVQGDEEWELRARIDGRVLKRVRARDLWQLIADSAWQCADPGLQYDTTINDWHTSPVS
                     GRINASNPCSEYVFLDNTACNLAHLNLMKFYDPKSGSFDVERYRHGIRLLTIVLEISV
                     LMAQFPSDAIARNSYDFRTLGLGYANLGSLLMTRGLPYDSDEGRAIAAALTAILTGEA
                     YAASAEMAQALGPFPRFEENRAAMLRVMRNHRRAAYNAHPGEYEGLSVTPVGLEPSAC
                     PTGLLQAARAAWDRALELGEQHGYRNAQTTLLAPTGTTGLLMDCDTTGVEPDFALVKF
                     KKLAGGGYFKIANQSIAPALETLGYSRAQIDDILRFVLGSLSLDGAPHVNRAALLARG
                     LTGEEIERVEQGLPRVFELSFAFTPWTLGEEAMARLGFTREQWQQPGFNLLRALGFSQ
                     AQIDEANDVVCGMQTVEGAPHLQAEHLPVFDCANRCGKHGQRFIHHLGHIRMMAAVQP
                     FLSGAISKTINMPNEVTADDIAEAYMASWRLGLKALALYRDGSKLSQPLSSGSTQAEK
                     AAEPAPRVETQVVVVEKPIRRLLPAKRRGFTQEGRVAGHKIYLRTGEYDDGALGEIFI
                     DMHKEGAAFRSLMNCFAIAISKGLQYGVPLDEFVDTFTFQRFEPQGFVEGHPNIKMAT
                     SIIDYVFRVLGYEYLGRTDFVQVKPIHDDEDEEFEDGDGAEADQPATHSAGPEESAPS
                     AAVASAPVTGSHANGNGYVARNGNGHGNGHGNGHGQPERQVLAEEYVTVAAAVVPQGD
                     GGLSEHLSQMMGDAPFCDVCGHITVRNGACYKCLNCGNSLGCS"
     gene            3861..4508
                     /locus_tag="VFU72_14230"
     CDS             3861..4508
                     /locus_tag="VFU72_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEU5424698.1"
                     /translation="MRRDKALAILAAHRDDLRERYGVKSLALFGSVARDEAGPASDID
                     LLVEFDRSIGLFHLFDTQHFLEDLLGRAVDLTPRNSIRPELRDIILAEAINAPREWRL
                     RITDILDAIARIQRYTADLTEDAFVNDERTRDAVIRNLEVIGEASRHVPDEIKAGHPE
                     IPWRSMLDMRNFAVHQYEQINLGIIWSTIQDDIPPLVPRLQRILAGTGEDTNAPR"
     gene            4495..4791
                     /locus_tag="VFU72_14235"
     CDS             4495..4791
                     /locus_tag="VFU72_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5424699.1"
                     /translation="MRRDDVLAILAAHRDELHEQFGVKSLALFGSVARDEAGPESDVD
                     LLVEFDRRPVGLFEFIDVKMALERLLGVPVDLATPGSLRERIRRSVERDVIHVA"
     gene            4781..5125
                     /locus_tag="VFU72_14240"
     CDS             4781..5125
                     /locus_tag="VFU72_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEU5424700.1"
                     /translation="MSPRNWQERIDDILDAVDEVERFVDGMSFEQFAQDTKTIYAVSF
                     AIATIGEAAGDIGPDIKLRHPDVPWGQMQAMRNVVIHQYFRIELTILWDTATANLPTL
                     IEPLRRLREAES"
     gene            5177..5470
                     /locus_tag="VFU72_14245"
     CDS             5177..5470
                     /locus_tag="VFU72_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5424701.1"
                     /translation="MRRDDVLAILAAHRDELRETYGVKSLALFGSVARDEAGPESDVD
                     LLVEFDRPTGLFGLAALQIFLEQVLGTGVDIGRSEALREYVRGSVEQDLVYVS"
     gene            5460..5807
                     /locus_tag="VFU72_14250"
     CDS             5460..5807
                     /locus_tag="VFU72_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEU5424702.1"
                     /translation="MSPRSLQARIDDMLTAATNAQSYVAGMSFEDFSQDLKTIRATAF
                     EITILGEAAGHIPPDIRARHPEIPWALMQRMRNLIAHGYFRIDTLTVWETDQDDLPHL
                     VLQLQGLRDELAT"
     gene            complement(5800..5949)
                     /locus_tag="VFU72_14255"
     CDS             complement(5800..5949)
                     /locus_tag="VFU72_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424703.1"
                     /translation="MEAIRRWFASLRQPGDVRRAAIAGIVFGLVMVMVVIGACLIINF
                     MASLR"
     gene            6121..7065
                     /locus_tag="VFU72_14260"
     CDS             6121..7065
                     /locus_tag="VFU72_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5424704.1"
                     /translation="MTERDVAALAHRIIESIERVVVGKRDVVELVLVALLCEGHVLLE
                     DVPGVGKTTLARAVARAIGGSFRRIQFTPDLLPSDVSGLTYYNQKLGEFQLRPGPIFA
                     SVLLADEINRATPRTQSALLEAMEERTVTIEGETLPLPRPFLVLATENPVELEGTFPL
                     PEAQLDRFLMRLSIGYPDEANELAMLARFQLGSPLDDAVPVIEPGELLAAAAACRQVH
                     VDDDLRRYITDITRASREHETVQLGVSPRGTLALFRAAQARAALSGRAYVLPDDVKAV
                     GQPVLAHRIVLSPEARLRGRDVREVIAEVMRSIAVPVE"
BASE COUNT         1232 a         2471 c         2356 g         1173 t
ORIGIN      
        1 gtattacact ggcacttggt tgtacgtaca gaacaggtgt tcaggacaac tcgtgacctg
       61 taggcaactc tagacagcga cccgcgcgcc tgctacacta tatctagtgg cggcagtggc
      121 tgcgagcaca agatgtcgcg ccagggccac gaagaacacc tcctcggcga gacaatcttc
      181 cggaattgtc tccagggcat ccgggccgat caccactcct cgtgatatcg gctggttttt
      241 ggcacaaagg atgcgtctgt cgtggggacg gcgcgccgca aggggcgggc gcatccgggc
      301 atcaacttct cggcggcggt tgggatctca gcgagggatt gacgggaaca tgaagaagcg
      361 accctctgcg cgcggcctgc gcgtcgcgcg tgtcttcacc tctccaggcc aggacccctt
      421 cgagacgatc agttgggcca ggcgcacctc gcgcatcacc aatccggatg gctcggtcgt
      481 cttcgagctg gcggacgccg agatcccggc gccctggtcg caggtcgcga ccgacatcat
      541 ggtctccaag tactttcgca aggcgggcgt gccccagcac gacaccgccg gcaacccgct
      601 gctcgacgcc gacggcgcgc cggtgctggg cgccgagcag agcgcgcggc aggtgatcgg
      661 ccggctggcc ggcacctggc gcgactgggg cgagcgctac ggctacttcg ccggcgaggc
      721 ggacgccgcg gccttcgagg acgagctgaa gtacatgctg gtgcaccaga tggcggcgcc
      781 caactcgccg cagtggttca acaccgggct gcactgggcc tatggcatca ccggcccggc
      841 gcagggccat gcctacgtcg accccgagag cggcgagctg cgccactccg aggacgccta
      901 tagccggccc accccgcacg cctgcttcat ccagtcggtc gaggacgacc tgatcaacga
      961 gggcggcatc ttcgacctgg tgacgcgcga ggcgcgcgtc ttcaagtacg gctccggctc
     1021 cggcaccaac ttctccaaga tccgcgccga gggcgagcag ctctcgggcg gcggcacctc
     1081 gtccggcctg atgagcttcc tcaagatctt cgaccgcgcc gccggggcga tcaagagcgg
     1141 cggcaccacg cgccgcgccg ccaagatggt ggtggtcgac atcgatcacc cggacatcga
     1201 gaagttcatc agttggaagg ccgaagagga gaagaaggtg gcggccctga tcgccgccgg
     1261 ctactccaac gacttcaacg gcgaggccta cgccaccgtc tccggccaga actcgaacaa
     1321 ctcggtgcgc gtcaccgacg acttcatcaa ggcggtgcag ggcgacgagg agtgggagct
     1381 gcgcgcccgc atcgacggcc gcgtgctcaa gcgcgtgcgc gcccgcgacc tctggcagct
     1441 gatcgccgac tcggcctggc agtgcgccga ccctggcctg cagtacgaca ccaccatcaa
     1501 cgactggcac accagccccg tgtcggggcg catcaatgcg tctaacccgt gttcagagta
     1561 cgtcttcctc gataacacgg cctgcaacct ggcgcacctg aacctgatga agttctacga
     1621 cccgaagagc ggcagcttcg acgtggagcg ctatcgccac ggcatccgcc tgctgacgat
     1681 cgtgctcgaa atcagcgtgc tgatggccca gttcccgagc gacgccatcg cgcggaacag
     1741 ctacgacttc cgcacgctcg gactcggcta cgccaacctc ggctcgctcc tgatgacccg
     1801 tggcctaccc tacgattcag acgaggggcg ggccatcgcc gcggcgctca ccgccattct
     1861 caccggcgag gcctacgccg cctcggccga gatggcgcag gccctgggcc ccttcccgcg
     1921 cttcgaggag aaccgcgcgg cgatgctgcg ggtgatgcgc aaccaccggc gagccgccta
     1981 caacgcccac cccggcgagt acgaggggct gtcggtgacc ccggtggggc tggagccgag
     2041 cgcctgcccc accgggctgt tgcaggccgc ccgcgccgcc tgggaccgcg cgctggagct
     2101 gggcgagcag cacggctacc gcaacgccca gacgacgctg ctggcgccga ccgggacgac
     2161 cgggctcttg atggactgcg acacgaccgg ggtcgagccc gacttcgcgc tggtcaagtt
     2221 caagaagctc gccggcgggg gctatttcaa gatcgccaac cagtcgatcg ccccggcgct
     2281 ggagacgctc ggctatagcc gggcgcagat cgacgacatc ctgcggttcg tgctcggcag
     2341 cctctcgctc gacggcgcgc cgcacgtcaa ccgcgccgcg ctgctggcca gggggctgac
     2401 cggcgaggag atcgagcgtg tcgagcaggg gctgccccgc gtcttcgagc tgtcgttcgc
     2461 ctttacgccc tggacgctgg gcgaggaggc catggcccgg ctcggcttca cccgcgagca
     2521 gtggcagcag ccgggcttca acctgctgcg cgcgctcggc ttcagccagg cgcagatcga
     2581 cgaggccaac gacgtcgtct gcggcatgca gaccgttgag ggcgcgccgc acctgcaggc
     2641 ggagcacctg ccggtcttcg actgcgccaa ccgctgcggc aagcacggcc agcgcttcat
     2701 ccatcatctg gggcacatcc gcatgatggc ggcggtgcag ccgttcctct ccggcgcgat
     2761 ctccaagacg atcaacatgc ccaacgaggt gacggccgac gacatcgccg aggcctacat
     2821 ggcctcctgg cgccttggcc tcaaggccct ggcgctctac cgcgacggct ccaagctgtc
     2881 gcagccgctc tcctcgggga gcacgcaggc cgagaaggca gcggagccgg cgccgcgggt
     2941 cgagacgcag gtggtggtgg tcgagaagcc gatccgccgc ctgctaccgg ccaagcgtcg
     3001 cggcttcacc caggagggcc gcgtcgccgg acacaagatc tacctgcgta ccggcgagta
     3061 cgacgacggc gcgctgggcg agatcttcat cgacatgcac aaggagggcg cggccttccg
     3121 cagcctgatg aactgcttcg ccatcgccat ctccaagggc ctgcagtacg gcgtgccgct
     3181 ggatgagttc gtcgacacct tcaccttcca gcgcttcgag ccgcagggct tcgtcgaggg
     3241 ccacccgaac atcaagatgg cgacctcgat catcgactac gtcttccgcg tgctcggcta
     3301 cgagtacctg ggccggacag acttcgtgca agtgaagccg atccacgacg acgaggatga
     3361 ggagttcgag gacggtgatg gcgcggaggc ggaccagccc gcgacccata gtgccggccc
     3421 ggaggagtct gccccgagcg ccgcggtggc gagcgctcct gtcaccggca gccacgccaa
     3481 cgggaacggc tacgtcgcgc gcaacgggaa cgggcatggc aacggccacg gcaacggcca
     3541 cggccagcct gagcgtcagg tgctggccga ggagtacgtc acggtggcgg cggcggtcgt
     3601 gccgcagggc gacggcgggc tgagcgagca cctgtcgcag atgatgggcg acgccccctt
     3661 ctgcgacgtc tgcggccaca tcaccgtgcg caacggcgcc tgctacaagt gtctcaactg
     3721 cggcaacagc ctcggctgct cctagccgcg ccgccgtcag tcggaagccc cggagcctga
     3781 gcgacgctca ggctccgcgc gtttgccggc tcgctatctc gctatactga ggctgacgac
     3841 ggcggagagg gaggttgccg atgcgccgcg acaaagccct ggccatcttg gccgcccacc
     3901 gcgacgacct gcgtgagcgc tacggcgtca aatcgctcgc cctcttcggc tcggtcgccc
     3961 gcgacgaggc cgggcccgcg agcgacatcg atctgctggt cgagttcgat cgatcgatcg
     4021 gtctgttcca cctcttcgat acccagcatt ttcttgagga tctcttgggg cgcgcggttg
     4081 atctcacccc gcgtaactca atccgtcccg agctgcgcga catcatcctg gcggaggcga
     4141 tcaatgcgcc tagagagtgg cgcctgcgca tcactgacat cctcgacgcg atcgccagga
     4201 tccagcgtta caccgctgac ctgactgagg atgctttcgt caacgacgag cgaacccgtg
     4261 acgccgtgat tcgaaacctc gaagtgatcg gtgaagcgtc acggcacgtc cccgacgaga
     4321 tcaaggccgg gcaccccgag attccgtggc gcagcatgct ggatatgcgg aacttcgcgg
     4381 ttcaccaata tgaacagatc aatttgggaa tcatttggag caccattcaa gacgatattc
     4441 ctccgcttgt cccgcgacta caaaggatct tggctggaac cggggaggac acgaatgcgc
     4501 cgcgatgacg tcctggccat cctggcggcc caccgcgatg aactgcacga gcagttcggc
     4561 gtcaaatcgc tggccctctt cggctcggtc gcccgcgacg aggccgggcc agagagtgat
     4621 gtcgatctgc tggtcgagtt cgaccggcgg ccggtgggcc tattcgagtt catcgacgtg
     4681 aagatggcgc ttgagcggct cctgggcgtg ccggtcgatc tcgccacgcc gggatcgctg
     4741 cgggagcgta tccggcgaag tgtcgagcga gacgtcatcc atgtcgccta ggaactggca
     4801 agagcgcatc gacgacatcc tcgatgcggt cgacgaggtc gagcgcttcg tcgacggcat
     4861 gagcttcgag cagttcgctc aggacacgaa aacgatctat gccgtcagct ttgcgattgc
     4921 gacgatcggg gaagcagcgg gcgatattgg accagatatc aagctgcggc accccgacgt
     4981 gccgtgggga cagatgcagg caatgcgaaa cgttgttatc caccagtatt tccggattga
     5041 attgacgatc ctttgggata cagccaccgc caatctccca acgttgatcg agccgctgcg
     5101 acggcttcgt gaggccgagt cgtgagtgcg ccactcacgg catccccagc ctgccaagca
     5161 ccgcctggag gtgaccatgc gccgcgatga cgtcctcgcc atcctggccg cccaccgcga
     5221 cgagttgcgt gagacctatg gcgtcaagtc cctcgccctc ttcggctcgg tcgcccgcga
     5281 cgaggccggg cccgagagcg acgtcgatct actggtcgag ttcgaccggc caactggact
     5341 cttcgggctc gccgctctcc agatctttct tgagcaggtt ctcggtaccg gggttgatat
     5401 tggacgttcg gaagctcttc gagagtatgt ccgcggcagc gttgaacagg atctcgtgta
     5461 tgtctcctag atccttgcag gcgcgcatcg acgacatgct cacggccgcc acaaacgctc
     5521 agagctatgt tgccggcatg tcgtttgagg atttctccca agatctgaag acgattcgtg
     5581 cgaccgcatt cgagatcacg atccttggcg aggccgctgg tcacatcccg cctgatatcc
     5641 gggctcggca tcccgaaatc ccatgggcac tgatgcagag aatgcgcaat ctcatcgccc
     5701 atggatattt tcggatcgat accctcaccg tctgggagac ggatcaagac gacttgccgc
     5761 acctcgtgct ccagcttcag gggctccgag acgagttggc tacctgaggc tcgccatgaa
     5821 gttgatgata aggcaggcgc cgatcacgac catgaccatc acgagcccga agacgatgcc
     5881 ggcgatggcg gcgcggcgca cgtcgcccgg ctggcgcagg gacgcgaacc agcggcgaat
     5941 cgcttccata aggcattccc ttctcggaca cagcggcgcg atgccggcgc cgtcgctcgc
     6001 cgcgcgcctc ccggcggcaa tctcgtgccg tcaatcatgc cgcgcgcgcg gactccgcga
     6061 ctaggctgct tccgtcaagt aggattaggg gagcatttgg acgcacgggc gggggcaggc
     6121 gtgaccgagc gggatgtcgc ggcgctggcg caccgcatta ttgaaagcat cgagcgggtc
     6181 gtcgtcggca agcgcgacgt ggtcgagctg gtgctggtgg cgctcctctg cgaggggcac
     6241 gtcctcttgg aggacgtgcc cggcgtgggc aagacgacgc tggcccgcgc ggtggcgcgc
     6301 gctattggcg gctccttccg ccgtatccag ttcacccccg atctcctccc ctccgacgtc
     6361 agcgggctga cctactacaa ccagaagctg ggtgagttcc agctgcgccc cggcccgatc
     6421 ttcgccagcg tgctcttggc cgacgagatc aaccgcgcca ccccgcgcac ccagtccgcg
     6481 ctgctggagg cgatggagga gcgcaccgtc accatcgagg gcgagacgct ccccctgccg
     6541 cgccctttcc tggtgctggc gaccgagaac ccggtcgagc tggagggcac cttcccgctg
     6601 ccggaggcgc agctcgaccg cttcctgatg cggctctcga tcggctaccc ggacgaggcg
     6661 aacgagctgg cgatgctggc gcgctttcag ctcggcagcc cgctcgacga cgcggtgccg
     6721 gtgatcgagc cgggtgagct gctggcggcg gccgccgcct gccgccaggt ccacgtcgat
     6781 gacgacctgc ggcgctacat caccgacatc acccgggcca gccgcgagca cgagacggtg
     6841 caactcggcg tcagcccgcg cggcacgctg gccctctttc gcgcggccca ggcgcgggcc
     6901 gcgctctccg gacgcgccta tgtgctcccc gacgacgtca aggccgtcgg ccagccggtg
     6961 ctggcccacc gcatcgtgct cagcccggag gcgcggctgc gcggccgcga cgtgcgcgaa
     7021 gtgatcgcgg aggtgatgcg atcgatcgcc gtgcccgtcg aatagagcga cccgaacccc
     7081 cgccggtcga gcagccggtg cgcagtctct tcttcagcgg cgtctggttc tatggcgccg
     7141 ccggcctcct gctgctcggc atggcgctgc gccagcccgc cctctcgatt ctcgccttcc
     7201 tgaccctgct gaccgccgcc agcagccggc tt
//
LOCUS       DASUBI010000730         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_668955, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000730 DASUBI010000000
VERSION     DASUBI010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668955"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..1735
                     /locus_tag="VFU72_14265"
     CDS             458..1735
                     /locus_tag="VFU72_14265"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEU5424705.1"
                     /translation="MRLTQPPEMEAVEYHHDAIVPSRRALAAFVAGYRVPLLAWVLHL
                     ALVFAVAAIGVANFYTLNAVPAIGFALPPMSGIAHYTIQPLRNWDGFWYALVALYGYG
                     VYQASAAFWPLYPFLLRIGVEVTNWSVPVVGVLISNLAFLGALCLLYKLVTLEVSRTV
                     AARTIWLIAFFPTAFFFSAVYTESLFLLVTVAAIYLARTGRWWWAGFFGFLAALTRNT
                     GILILIPLGLLLLQERGWDPRRWWRQALGLTPVLLGPLLFFAELKHVWGDPLLTLHAQ
                     KGWARYRAMPWETLQTEWHKLDLTWFWYLVQHPTWDVLSNPNIRYLFAERQAYDLFIF
                     VLFVPLTLYMLWRERPAYSLYALVSFVVPLFSPSLVHPLMSYPRFVIVLFPFFIALAR
                     LTRNRWVFSLLLALCLVQFAGLLIQFSTWFWVA"
     gene            complement(1936..2859)
                     /locus_tag="VFU72_14270"
     CDS             complement(1936..2859)
                     /locus_tag="VFU72_14270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU5424706.1"
                     /translation="MDDSSASNSHCTAAHDGPCPPGTVILADNLAVLPRLPDASIPLI
                     YIDPPFNTGHSQSHTRLRMERDPEGSRTGFQGRSYRAIPLGTSQFADDFDDYLGFLEP
                     RLRQAHRLLSENGTLYVHLDPREVHYVKVLLDTIFGRASFLNEIIWAYDFGGRPRRRW
                     PAKHDNILVYVKNPADYVFNVDAIERIPYLAPGLVGPEKAARGKRPTDVWWHTIVPTS
                     GRERTGYPTQKPLAIPRRIIAASSNPGDLVLDFFAGSGTTGAAARALGRRFLLVDNNP
                     EAFGVMRQRLAFEGVRFFETPKDWTPLGSGE"
     gene            2860..3864
                     /locus_tag="VFU72_14275"
     CDS             2860..3864
                     /locus_tag="VFU72_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424707.1"
                     /translation="MYTPGFAILTSLLMAIISIRTLYVVARSWRQLFDQHFTPAERQL
                     VSQAAFFLLLPLSVPLHEAGHAVAVKLFGGQITGYGWYVFYGFVEYVGVRSASAVFWI
                     ALAGNLVSVALGAAAIAVALFRPRRAAINYLLLVFGALSILNALVFYPLLDLIGGFEG
                     DWSQIYSGRTPALSLGTAIAHGAILLAMLLVWRSDRFRRLYAERTGLRPDAMRRVSRA
                     QAADELLSAGEQLSASWRHPLRVVAEAPENAAGVSLHWISNGYGRVVAAVAVLDGQRR
                     IELYGGLKVLDGSGRHFERPLGYVQGIPAPDQVTPALVRALDLVDAWDARTVEQPIEG
                     "
BASE COUNT          525 a         1315 c         1336 g          702 t
ORIGIN      
        1 gtcagatgcc agcactcgtg cggcccaccg gccgcgcggc agagctcaac ctcggccgcg
       61 gtgcggaccg agtagccgag cgagcagcgc atcaccggac ggccattccc gttgagatgg
      121 agctgtgtgc gtcggagcat cgaacatcca agctggagct caccgacttc gtcttgagct
      181 ggcgccgggt gcgacccagg acgatcttcc cgtgatcgaa ccatggcacc gttcactttc
      241 cggccggtcc gcggtccacc atgatccccc aggcggccgc cgcgccggca cgtgcgttgc
      301 gaacaatcgc ggatcgcgac cgtccgccgc gctcaagtat acacgccggt ccagttcctc
      361 ttctccaatg gtccgggcgc gggcggcgtg gcatgattgg ccgcgcgcgg ccggaagcgg
      421 ttccgcccgt gcctgttgag gagggcgacc cagttccatg cgcctcaccc agcctcccga
      481 gatggaggcg gtggagtatc accacgacgc catcgtaccc agcagacgcg ccctcgccgc
      541 cttcgtcgcc ggctaccgcg tgccgctgct ggcctgggtg ctgcacctgg cgctggtctt
      601 cgccgtggcg gcgatcggtg tagccaactt ctataccctc aacgcggtcc cggccatcgg
      661 cttcgcgctg ccgccgatga gcggcatcgc ccactacacg atccagccgc tgcgcaactg
      721 ggatggcttc tggtacgcgc tggtggcgct ctacggttac ggcgtctacc aggccagcgc
      781 cgccttctgg ccgctctacc cgtttctctt gcgcatcggg gtggaagtca ccaactggag
      841 cgtgccggtc gtcggcgtgc tgatctccaa cctggccttc ctgggcgcgc tctgcctgct
      901 ctacaaactg gtgacgctgg aggtgagccg gacggtggcg gcacgcacca tctggctgat
      961 cgccttcttc ccgacggcct tcttcttctc ggcggtctac accgagtcgc tcttcctgct
     1021 ggtgacggtg gcggcgatct acctggcgcg caccgggcgc tggtggtggg ccggcttctt
     1081 cggcttcctg gcggcgctga cgcgcaacac cggcatcctg atcctgatcc cgctcgggct
     1141 gctgctgctc caggagcgcg gctgggatcc gcgccgctgg tggcgccagg cgctggggct
     1201 gacgccggtg ctgctcgggc cgctgctctt cttcgccgag ctgaagcacg tctggggcga
     1261 tccgctcttg acgctgcacg cgcagaaggg ctgggcgcgc taccgggcga tgccctggga
     1321 gacgctgcag accgagtggc acaagctcga cctcacctgg ttctggtatc tggtgcagca
     1381 tccgacctgg gacgtgctga gcaacccgaa catccgctac ctcttcgccg agcgccaggc
     1441 gtatgacctc ttcatcttcg tgctcttcgt gccgctcacg ctctacatgc tctggcgcga
     1501 gcgcccggcc tacagcctct acgcgctggt gagcttcgtg gtgccgctct tcagcccgtc
     1561 gctggtgcac cccctgatgt cctacccacg cttcgtgatc gtcctcttcc ccttcttcat
     1621 cgcgctggcc aggctgacgc gcaaccgctg ggtcttcagc ctgctgctgg cgctctgcct
     1681 ggtgcagttc gccgggctgt tgatccagtt cagcacctgg ttctgggtgg cgtaggcgca
     1741 ttcggctgcc catcgaatgg ggtaggcggc aggtgaggaa ggatccctcg actgcgctca
     1801 gagcctgccc tgagcctgcc gaagggatgt cagggtaagg gatgaggcct tgcggacgag
     1861 gacagtcctc gtcgtctgcc ccccaactaa gcctgtcatc ctgagcttgt cgaaggatcc
     1921 gtgctggcga ggcactcact ctccgcttcc aagcggcgtc caatccttcg gcgtctcgaa
     1981 gaaccgcacc ccctcgaagg cgaggcgctg gcgcatcact ccgaaggctt cagggttgtt
     2041 atccaccagc aggaagcggc ggccgagcgc gcgcgcggcg gcgccggtgg tgccgctgcc
     2101 ggcgaagaag tcgagcacca ggtcgccggg gttggaggag gcggcgatga tccggcgcgg
     2161 gatcgccagc ggcttctgtg tcgggtagcc ggtgcgctcg cgcccgctgg tcggcacgat
     2221 ggtgtgccac cagacgtcgg tcggccgctt gccgcgcgcc gccttctccg gccccaccag
     2281 ccccggcgcc aggtagggga tgcgctcgat ggcgtcgacg ttgaagacgt agtcagcggg
     2341 gttcttgaca taaacgagga tgttgtcgtg cttggccggc cagcggcggc gcgggcgccc
     2401 gccgaagtcg taggcccaga tgatctcgtt gaggaacgag gcacggccga agatggtatc
     2461 gagcagcacc ttgacgtagt ggacctcgcg cgggtcgagg tggacgtaga gggtgccgtt
     2521 ctcgctcagc aggcggtgcg cctggcgcag gcgcggctcc aggaagccga ggtagtcgtc
     2581 gaagtcgtcg gcgaactgac tggtgccgag cgggatcgcg cggtagctgc gcccctggaa
     2641 gccggtgcga ctcccttcgg gatcgcgctc catgcgcagc cgcgtgtgcg actggctgtg
     2701 gccggtgttg aaaggggggt cgatgtagat cagcggaatg gaggcgtccg gcaggcgggg
     2761 taggacggcg agattgtcgg ccaggatgac ggtgcccggc ggacaggggc cgtcgtgggc
     2821 tgcggtacaa tgactgttcg aggcagagga gtcgtccacg tgtacacacc cggcttcgcg
     2881 attctcacca gcctgctgat ggccatcatc tccatccgca cgctgtacgt cgtcgcccgc
     2941 tcctggcggc agctcttcga ccaacacttt accccagccg agcgccagct cgtcagccag
     3001 gcggcgttct tcctcctgct gcctttgagc gtgccgctgc acgaggccgg gcacgcggta
     3061 gcggtcaagc tcttcggcgg ccagatcacc ggctacggct ggtacgtctt ctacggcttc
     3121 gtcgagtacg tcggggtgcg cagcgcgagc gccgtcttct ggatcgcgct ggccgggaat
     3181 ctggtgagcg tcgcgctggg cgcggcggcg atcgcggtgg cgctcttccg cccgcggcgg
     3241 gcggcgatca actacctact gctggtcttc ggcgcgctga gcatcctcaa cgcgctggtc
     3301 ttctatccgc tgctcgacct gatcggcggc ttcgagggcg actggtcgca gatctacagc
     3361 gggcgcacgc ccgcgctcag cctgggcacc gccatcgccc acggcgccat cctgctggcg
     3421 atgctgctgg tttggcgcag cgaccgcttc cgccgcctct acgccgagcg caccgggctg
     3481 cgccccgacg cgatgcggcg cgtcagccgc gcccaggccg ccgacgagct gctgtcggct
     3541 ggcgagcagc tctcggcgag ctggcgccac ccgctgcggg tggtggccga ggcgccggag
     3601 aatgccgccg gggtctcgct ccactggatc agcaacggct acgggcgcgt ggtggcggcg
     3661 gtggcggtgc tcgacggcca gcggcgcatc gagctgtacg gcggcttgaa ggtgctcgac
     3721 ggctccggcc gccacttcga gcgcccgctc ggctatgtcc aggggatccc ggcgcctgat
     3781 caggtgacgc cggccctggt gcgggcgctc gacctggtcg acgcctggga cgcgcgcacc
     3841 gtcgagcagc cgatcgaagg ttaattccag gttcgaac
//
LOCUS       DASUBI010000731         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_776258, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000731 DASUBI010000000
VERSION     DASUBI010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776258"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFU72_14280"
     CDS             <1..604
                     /locus_tag="VFU72_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424708.1"
                     /translation="DATHPRPDRLAYVLDVDSGQARWVSPDRQLDDWTRQFFPANAAP
                     GDYSSLLGTTRAYTNPAPVVALAPPEVAVEHDAISDAGRQLELRLSSPRGVTEMTTVV
                     RAPGEIVAAAVDGRPLDLSAYPDARRGELRLIYADVQPAGWRLELSVRASAPIRIDLE
                     EATDGLPAVSGMTIAPRPADTMPAFDYPRDPTIVTRSLSF"
     gene            719..1651
                     /locus_tag="VFU72_14285"
     CDS             719..1651
                     /locus_tag="VFU72_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5424709.1"
                     /translation="MSRKHAFVVFLVSLVLALLPLVALAADGTNDSGELLLRINGPVT
                     VSQNETHDAVIVISDNATVDGTVTSALLVIDGDAVVSGRVDGDVTVVNGTLTLASTAQ
                     VKNVSSVRGNLNQDPAATVSGKISRSDLVVDAWVWGVISAILWIGMTIAVLAGGLIYA
                     AIAGRQLKATGDLIVSQPGPTLLAAALLWIALPLLMAAVIATIVGIPFGLGYFLVVLP
                     VVWFLGYLVAGTQLGRLIMGRRRDAERPYLAALLGLLLLQVLGLIPWLGVFSFLAGIV
                     GAGALALMAWHAWRKPGARPAPRLTQGPTPSPGG"
     gene            1894..2163
                     /locus_tag="VFU72_14290"
     CDS             1894..2163
                     /locus_tag="VFU72_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5424710.1"
                     /translation="MCRSIKTLYNLDPPAADDEVRAAAQQFVRKLSGFSAPSKANEAA
                     FAAAVDDITLAARRLLGALETNAPPRDREALAARARANAARRYTG"
     gene            2213..>3052
                     /locus_tag="VFU72_14295"
     CDS             2213..>3052
                     /locus_tag="VFU72_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU5424711.1"
                     /translation="MTNDTVALAHRQVAAVYQAESRRVFATLVRLLGDFELAEDGLHD
                     AFAAALEQWPRDGAPANPRAWLVSAGRFKAIDTIRRRARFDASLGALAQRIERDGGDW
                     EVEDVEDDRLRLIFTCCHPALAPDARVALTLREVCGLTTEEIARAFLTAPATLAQRIV
                     RAKRKIRDAGIPYRVPARAELPERLDAVLRVVYLVFNEGYAASSGDALTRPDLCGEAI
                     RLGRLLAELLPEPEVYGLLALMLLHESRRAARTTPEGDLVLLEDQDRSLWNRAQISEG
                     TALV"
BASE COUNT          437 a         1109 c         1052 g          454 t
ORIGIN      
        1 cgacgcgacc cacccgcggc ccgaccgcct cgcctacgtg ctggacgtcg atagcgggca
       61 ggcgcgctgg gtgagcccgg accggcagct cgacgactgg acgcgccagt tcttcccggc
      121 caacgccgcg ccaggcgact acagctcgct gctgggaaca acccgcgcct atacgaaccc
      181 tgcgccggtc gtcgcgctgg cgccgcccga agtcgccgtc gagcacgacg cgatcagcga
      241 cgctgggcgc cagctcgaac tgcgcctgtc gtcgccgcgc ggcgtgaccg agatgacgac
      301 ggtcgtgcgg gcgccgggcg agatcgtggc ggcggcggtc gacggacgcc cactcgatct
      361 cagcgcttat ccggacgcgc ggcgcggcga gctgcggctg atctacgccg acgtccagcc
      421 cgccggctgg cgtctggagc tgtcggtgcg cgcgagcgct ccgattcgga tcgacctcga
      481 agaggcgacc gacggcctgc cggccgtctc gggcatgacg atcgcgccgc gcccggccga
      541 caccatgccg gcgttcgact acccgcgcga tccgaccatc gtcacccgct cgctgagctt
      601 ctagggaagc gaggtgagcg gcctatgcca ccacggaggg ggcgaatacc gttgctgccg
      661 gcctggtaag cccaccgatt cgtccggctc gacgtcgatc atcagagagg agacaaccat
      721 gagccggaaa cacgcgttcg tcgtcttcct cgtgagtctg gtactcgcgc tcctaccgct
      781 ggtcgcgctc gccgcggacg gcacgaacga cagtggggag ttgctgctgc gcatcaatgg
      841 accggtcacg gtgagccaga acgagacgca cgacgccgtc atcgtgatca gcgacaacgc
      901 caccgtggac ggcacggtca cgagcgcgct gctcgtgatc gacggcgacg ccgtggtgag
      961 cgggcgggtc gatggtgacg tgaccgtcgt caacggcacg ctgacgctgg cctcgaccgc
     1021 gcaggtcaag aacgtctcct cggtacgggg caacttgaac caggatcccg ccgccacggt
     1081 gagcggcaag atctcccgca gtgatctggt cgtcgacgct tgggtctggg gcgtcatctc
     1141 cgcgatcctc tggatcggga tgacgatcgc cgtactggcc ggcgggttga tctacgcggc
     1201 catcgccggt cgccagctca aggcaaccgg cgatctgatc gtcagccagc cggggccgac
     1261 gctgctggct gccgcgctgc tctggatcgc gctgccgctg ctgatggcgg cggtcatcgc
     1321 cacgatcgtc ggcatcccct tcggcctggg gtacttcctg gtcgtcctgc cggtggtctg
     1381 gttcctcggc tacctggtag ccgggacgca gctcggccgc ctcatcatgg gccggcgtcg
     1441 cgacgccgag cgtccctacc tcgccgcgct gttgggtctg ctgctgctgc aggtgctcgg
     1501 cctgatcccc tggctgggtg tcttctcctt cctggcgggg atcgtcgggg ccggtgccct
     1561 ggccctgatg gcctggcatg cctggcgcaa gccgggggcc cgtccggcac cgcggctgac
     1621 gcaggggccg acgccctccc cgggcggcta gactgcctga tagcgaacga ggagcgtggg
     1681 aaaccgcgct cctcgttccc cccatgtcaa cggcaccgtg caccgtgcgc gcgccccacg
     1741 ctaccatcgc gccgctcacc caggtacact tccgccgccg ggttgagaaa tcgaacaatt
     1801 gttctataat gggttcaggc gaggcacccg gcgctcgccg gcagcaggcg cgttgaacgt
     1861 ctggtgcgcc acgatcgcgg aagggaccag gggatgtgcc gcagcatcaa gacgctctac
     1921 aacctcgacc caccggccgc ggacgacgag gtccgcgccg ccgcacagca gttcgtgcgc
     1981 aagctcagtg gcttcagcgc gccctccaaa gccaatgagg ccgccttcgc ggccgcggtc
     2041 gacgacatca cgctggccgc gcgacggctg cttggcgcgc tggagacgaa cgcgccgcca
     2101 cgggaccgcg aggcgctggc cgcccgcgcc cgcgccaacg ccgcccgtcg ctacaccggc
     2161 tgagccggcg ccccagcgtc tgacagacaa caagcgcaag gattcgatgc cgatgaccaa
     2221 cgacaccgtc gcactggcgc accggcaggt agcagcggtc taccaggccg agtcgcgccg
     2281 cgtcttcgcc acgctggttc gcctgctggg cgacttcgag ctggccgagg atggcctgca
     2341 cgacgccttc gccgccgcgc tggagcagtg gccgcgcgac ggcgcgccgg ccaacccgcg
     2401 cgcctggctg gtctcggccg ggcgcttcaa ggccatcgac accatacgcc ggcgcgcccg
     2461 cttcgacgcc tcgctggggg cgctggccca gcgcatcgag cgcgacggcg gcgattggga
     2521 agtcgaggat gtcgaggacg accggctgcg cctgatcttc acctgctgcc accctgccct
     2581 cgcgcccgac gcccgcgtgg cgctgacgct gcgcgaggtc tgcgggctga ccaccgagga
     2641 gatcgcccgc gccttcctca ccgcgccggc cacgctggcc cagcggatcg tgcgtgccaa
     2701 gcgtaagatc cgcgatgccg gcatccccta ccgcgttccc gcccgcgccg agttgcccga
     2761 gcggctggac gccgtgctgc gcgtcgtcta cctggtcttc aacgagggct acgccgcctc
     2821 ctccggcgat gcgctgaccc ggcccgacct ctgcggcgag gcgatccggc tggggcgtct
     2881 gctcgcggag ctgctgcccg agccggaggt ctacgggctg ctggcgctga tgctgctgca
     2941 cgagtcgcgc cgggcggcgc gcaccacgcc cgagggcgac ctggtgttgc tggaggatca
     3001 agaccgctcc ctctggaacc gcgcgcagat cagcgagggg acagcgctgg tg
//
LOCUS       DASUBI010000732         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1188826, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000732 DASUBI010000000
VERSION     DASUBI010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188826"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFU72_14300"
     CDS             <1..1146
                     /locus_tag="VFU72_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEU5424712.1"
                     /translation="GWQVESGNAGQHYLQWRYGDPVLRQQNLKRATAAYQAMQQQEYQ
                     ADVALYHELGSRDYAYHWPFSQASAATIDLAGLPDAGGSYLSDLQQRLTGLDHYWDAT
                     HQPPGLSSAVMPPLGPSGDLFYDDNAWTGLALVQAYRETGNPDLLERARRILQFDLSG
                     WDNDASHPAPGGVFWVDAGWNRDRNTVSTAGTAKLALQLADVTSDPAQRAGYLAWGQK
                     LTGWVEQTLRAPDGLYWDHIDLAGKIDKTEWSYNQGQMIGAYVLLYRLTGDQNDRQRA
                     QAIANAALAFYVDGGRLDAQPPIFNAIFFENLQFLATVDANPRYTWEAQRYADWLWTT
                     RRDPATNLFAGASEPGSSANDALLDQAAVIQLYARLAWDPGSYGLSR"
     gene            1246..2151
                     /locus_tag="VFU72_14305"
     CDS             1246..2151
                     /locus_tag="VFU72_14305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5424713.1"
                     /translation="MRFGVSLPTYPAGATIEGFTAVAQAAEPLGFVSAWATDHVILPA
                     DHSGPYGSIFEPLATLAYLAALTSTVKLGVSVIVVPQRNGVLLAQELATLDQLARGRL
                     IAGVGVGWSEPEFTMLGAGSIFHERGKVLDETLQLWQHLWSEPQTPFHGRYYDLPAVA
                     FGPLPVQPGGPPIWVGGSAEETLRRTVRFGRAWHPVGASVEQVAAGAARLRELAQAAG
                     KPVPEIAPRLPLFIGRRASAAPMSANPQRVLEGEPHDLIAALRAYQEAGAGEIICHFG
                     TPPGGSVVEQMARFQREVMPAFTGS"
     gene            complement(2303..2710)
                     /locus_tag="VFU72_14310"
     CDS             complement(2303..2710)
                     /locus_tag="VFU72_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424714.1"
                     /translation="MATIGTRFHRVELSSIALVASGILVGALLVGGISRLRPTSRPHA
                     VSQTAASLTQSSAPAASAPASQMPIPDFGFVPGTDGPADAASVAATTASSQAPIPATG
                     VVPGTAVPAGSANNAAGNAAPNRIANPELGPIP"
     gene            complement(2909..3424)
                     /locus_tag="VFU72_14315"
     CDS             complement(2909..3424)
                     /locus_tag="VFU72_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424715.1"
                     /translation="MAIRGRIRVTAALLGLALLTPLQASAALLVPFQATVYEHYTVSV
                     CAPDTRCITATGSGEATQLGQVTQTSTVRVDLNPAHAVNGCVPETRETVLVASNGDEL
                     TMSAGGWGCPASSSAQDQYTVTGGTGRFQGATGSGMDVNVHTLSSASGGVAVTTYSGT
                     LSSPGSLKPAR"
     gene            3672..6176
                     /locus_tag="VFU72_14320"
     CDS             3672..6176
                     /locus_tag="VFU72_14320"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424716.1"
                     /translation="MAEPASESLADLLRRHRAAAALTQEELAERAGLSARAISDLERG
                     IKRRPHPYTVQQLIQALGLGDEEAAHLRRAARGAGSDRHGPGAPGEVGGPGAFLPIPP
                     TPFIGRRQEMTEVSALLRREEVRLLNLTGAGGIGKTRLALEVARQALEAFPDGVFFVW
                     LAPLADPALVPVTVAGALGIDEMGGQPVLETLRERLRARSVLLILDNFEHLLTAGTTL
                     SYLLATCPRLKLLVTSRASLQLAAEHEYLVPPLAVPASEHRPALGTEALSRCDAVQLF
                     VQRAQAIQAGFQLTGENGPAVAEICRRLDGLPLAIELAAARIRLFAPGALLARLADPL
                     RLLVGGARDLPARQRTLRDTIDWSYGLLAPAEQALLARLAVFAGGATLEAIEAVCGEG
                     NGQARDGLGQLDSLVRASLLRIEEDGTGSGRFQMLRTIREYAWERLAARGEAAALRQR
                     HAAYFAELAEQAEPAMWGGPEQHAWLRRLELEHDNLRAALASAEEAGEAHLHLRLAAS
                     LGRFWFIHGHATEGRRRLERALAAGPEADASLRARVLVALGMLARQQADPVSAVAYFE
                     ESLSLFRALGDERWLAFTLFGLGNVWQVWGDWRRGAELLEASLALSQGRGGHWGFVRG
                     LPLVMLGWLLLEQGEQARALPLLQEALALGTRLGDQVASGTALIGLGWAAHFAGDDRR
                     ALALLEEALGLFRALGHHQGRLDALASLGWVSLQGEDDERAAALFREALTLADESGYR
                     AYAGDCLRGLGGLAGLRGEARLAAELFGAAEGLRERAGTPPYPSGAAYRRAAAAVRAE
                     LGDAAWEEATAAGRSRPIPETIAAALAAATPASGGR"
BASE COUNT          883 a         2289 c         2339 g          983 t
ORIGIN      
        1 ggctggcagg tggagtcggg caacgccggc cagcactacc tccagtggcg ctacggcgat
       61 cccgtcctgc gtcagcagaa cctgaagcgt gccacggccg cctaccaggc gatgcaacag
      121 caggagtacc aggccgatgt cgcgctctat cacgagttgg gcagccgcga ctacgcctac
      181 cactggccct tttcgcaggc cagcgccgcc acgatcgatc tggcggggct gccggacgcg
      241 ggcggaagct acctgtccga tctccagcag cggctgaccg gcctcgacca ctactgggac
      301 gcgacgcatc agccgccggg gctctcctcg gcggtcatgc cgccgctcgg cccgagcggg
      361 gatctcttct acgacgacaa cgcctggacc ggcctggcgc tggtgcaggc ctaccgcgaa
      421 accggcaacc ccgatctgct ggagcgcgcc cgccggatcc tccaattcga cctctccggt
      481 tgggacaacg acgcgagtca tccggcgccg ggcggcgtct tctgggtcga cgccggctgg
      541 aaccgcgacc gcaataccgt ctcgaccgcc gggaccgcca agctggcgtt gcagttggcg
      601 gacgtgacca gcgatcccgc gcagcgcgcc ggctatctcg cctggggcca gaagctgacc
      661 ggctgggtcg agcaaaccct gcgcgcgccc gacggtctct actgggacca tatcgacctg
      721 gcaggcaaga tcgacaagac tgagtggagc tataatcagg gccagatgat cggcgcctat
      781 gtcctcctct accgcctgac cggcgaccag aatgaccggc agcgcgctca ggcgatcgcc
      841 aacgccgcgc tggccttcta cgtcgacggc ggcaggctgg atgcgcagcc gccgatcttc
      901 aacgccatct tcttcgagaa cctgcagttc ctcgccacgg tcgacgccaa cccgcgctac
      961 acgtgggagg cgcagcgcta tgccgattgg ctctggacca ccagacgcga cccggcgacc
     1021 aatctcttcg ccggcgccag cgagccgggc agcagcgcca acgacgccct gcttgaccag
     1081 gcggcggtca tccagctcta cgcacggctg gcctgggatc cggggagcta cgggctctcg
     1141 cgctgagcgg ccgcgagccc tcagcgagaa tcggtcttcc ctgccgcgga cgctccgcgt
     1201 aagatcggcg gcaggttggt cagggagcgg aaggaatagg cactcatgcg ctttggggtt
     1261 tcgttgccga cctatccggc gggggcgacg atcgagggct tcacggcggt ggcgcaggcg
     1321 gcggagccgc tcggcttcgt ctcggcctgg gcgaccgacc acgtgatcct gccggccgac
     1381 cacagcggcc cctacggctc gatcttcgag ccgctggcga cgctcgccta cctggcggcg
     1441 ctgacctcca ccgtcaagct cggcgtctcg gtgatcgtgg tgccgcagcg caacggggtg
     1501 ctgctggccc aggagctggc cacgctcgac cagctcgcgc gcgggcggct gatcgcagga
     1561 gtcggagtcg gctggagcga gcccgagttc accatgctcg gcgccgggag catcttccac
     1621 gagcgcggca aggtgctcga cgagacgctc cagctctggc agcacctctg gagcgagccc
     1681 cagacgccct tccacggccg ctactacgac ctgccggcgg tcgccttcgg cccgctgccc
     1741 gtgcagccgg gcggcccgcc gatctgggtc ggcggctcgg ccgaggagac gctgcgccgc
     1801 acggtgcgct tcggccgggc ctggcacccg gtcggcgcct cggtcgagca ggtggcggcc
     1861 ggggccgcgc ggctgcgcga gctggcccag gcggccggca aaccggtgcc ggagatcgcg
     1921 ccgcggctgc cgctgttcat cgggcggcgg gcgagcgcgg cgccgatgag cgccaacccg
     1981 cagcgcgtgc tggagggcga gccgcacgac ctaatcgccg cgctgcgggc ctaccaggag
     2041 gccggcgcgg gcgagatcat ctgccacttc ggcacgccgc ccggtggctc ggtggtggag
     2101 cagatggccc gcttccagcg cgaggtgatg ccggccttca ccggctcgta gcgatggagc
     2161 ccggtagttg gggcagccga cggcgccgcc ggtcatccca gaccagaaaa gcgccggtcc
     2221 ggatacgcca ccggaccggc gctttcggtc gctgatgctc aggattcctg ctcgctccca
     2281 acctaccggg cggtccctcc ggttacggga ttggccctag ctccgggttg gcgatccggt
     2341 tgggcgcggc gttgcccgcc gcgttgttgg ctgagccagc cggaacagcg gtgcccggca
     2401 caacgccagt ggctgggatt ggtgcctgac tggaggcggt ggtcgccgcg acgctcgccg
     2461 cgtcagccgg gccgtccgta cccgggacga agccgaagtc tgggatcggc atttggctgg
     2521 cgggagccga cgcggccggc gcactgctct gggtcaggct cgccgcggtc tgagacacgg
     2581 cgtgcggccg gctcgtgggc cggagccggc tgatgccgcc caccagcagc gctccgacga
     2641 ggattccgct ggccaccagg gcgatcgagc tcaactcgac ccggtgaaag cgcgtgccaa
     2701 tcgttgccat gactctctct ccctaacttt cctggctatc cccgtctggg gccacagacg
     2761 cccgagacga acccgtggat atgattggcg attggggccc agccgcgcct cgcggcggtt
     2821 ctccatgaag gctgcgtcgg ccctggtgtc gttcagcttc ggttccgctc acgtctgaac
     2881 gctcctcgct taatgtgcct gatccaggct agcgcgctgg cttcagcgag ccgggggatg
     2941 agagcgtccc gctgtaggtc gtcaccgcca cgcctccgct cgcgctgctg agtgtgtgga
     3001 cgttgacatc catacccgac ccggtggcgc cctggaagcg gccggtgccg ccggtgacgg
     3061 tgtactgatc ctgggcgctg ctgctcgccg ggcaacccca gccgccggcg ctcatcgtca
     3121 gctcgtctcc gttgctggcc accagcaccg tctcgcgcgt ctcggggacg cagccgttca
     3181 cggcatgggc cgggttgagg tccacgcgaa cggtggaggt ctgcgtcacc tggcccaact
     3241 gcgttgcctc cccgctgccg gtggcggtga tgcagcgcgt atccggcgcg cagacgctga
     3301 cggtgtagtg ctcgtagacg gtggcctgga agggcaccag cagtgcggcc gacgcctgca
     3361 ggggagtcaa gagtgccagc cccagaagcg cagcggtgac ccggattcgt ccgcggattg
     3421 ccatggtcga tctcctctcg tctgatcggt cgctcgcgag cgcgacacac cgcgatctcg
     3481 cttgcgtcca gtctgggcga cgaccggcgc gacagccacg aacgatcccg cacattcacc
     3541 cacacgatct gatggtctgg attcggctcc actgctacac tccgttcatc tcccagcagg
     3601 cccgccacgc tcgtgagccg ggctgacgag ggaacgcaga cgactcccgt gacggaagcg
     3661 agcgagacgg aatggccgag ccggcttcgg aatccctggc cgacctgctc aggcgccacc
     3721 gcgcggccgc cgcgctgacg caagaggagc tggcggagcg agcgggcctc agcgcccgcg
     3781 ccatcagcga tctggagcgg gggatcaagc gacgccccca tccctatacc gtccagcagt
     3841 tgattcaggc actcggcctg ggcgatgagg aagcggccca cctgcggcgg gccgcgcgtg
     3901 gagccggcag cgaccggcat gggcctgggg cgccgggcga ggtcggcggg ccgggcgcgt
     3961 tcctcccgat tccgcccacc ccgttcatcg gccgacggca ggagatgacg gaggtgagcg
     4021 ccctgctccg gcgtgaggag gtgcgcctgc tgaacctcac cggcgcgggc ggcatcggca
     4081 agacgcggct tgcgctggaa gtcgcgcggc aggccctgga ggccttcccc gacggcgtct
     4141 tcttcgtctg gctcgccccc ctggccgacc cggccctcgt ccctgtcacc gtggccggcg
     4201 cgctcgggat cgatgagatg ggcgggcagc cggtgctgga gactctgcgg gagcgattgc
     4261 gcgcccgatc tgtcctgctc atactggata acttcgagca cctgctgaca gccgggacca
     4321 cgctgtcgta cctgctcgca acctgtccgc ggctgaagct gctggtcacg agccgggcgt
     4381 ccctccagct cgcggccgag cacgagtatc tcgtgccgcc gctggccgtg cccgcttctg
     4441 agcaccggcc ggctctgggg acggaggcgc tgtcccgctg cgacgcggtg cagctcttcg
     4501 tccaacgcgc gcaagcgatt caggcgggtt ttcagctgac gggggagaac gggccggcgg
     4561 tcgcggagat ctgccgccgg ctggatggac tgcccctcgc catcgagctg gcggcggcgc
     4621 gtatccggct cttcgcgcca ggcgctctcc tggcgcggct cgccgacccc ttacgactcc
     4681 tcgtcggcgg ggcgcgcgac ctgccggcac ggcagcggac gttgcgcgat accatcgact
     4741 ggagctatgg cctgctcgct ccagcggagc aggcgctgct cgcgcggctg gcggtgttcg
     4801 ccggtggtgc aaccttggag gccatcgagg ccgtgtgcgg cgaggggaac gggcaggcgc
     4861 gcgatggcct ggggcagctc gactcgctgg tgcgcgccag tctcctgcgg atcgaggagg
     4921 acggcacggg ctccgggcgt ttccagatgc tgcggacgat ccgcgaatac gcctgggagc
     4981 ggctggctgc gcgcggcgag gcggcagccc tgcggcagcg gcacgcggcg tacttcgccg
     5041 agctggccga gcaggccgag ccggcgatgt ggggcggacc ggagcagcac gcctggttga
     5101 ggcgcttgga gctggagcac gacaacctgc gcgcggcgct ggcttcggcc gaggaagccg
     5161 gcgaggcgca cctgcacctc cgcctggctg cctcccttgg tcggttctgg ttcatccacg
     5221 ggcacgcgac cgagggtcgc cgccgcctcg agcgggcgct ggccgctggc ccggaagctg
     5281 acgcctccct gcgcgccagg gtgctggtcg cgctcggcat gctcgcgcgg cagcaggccg
     5341 acccggtcag tgccgtggcg tacttcgagg agagcctgtc cctcttccgc gcgctgggcg
     5401 atgagcgctg gctggcgttc acgctcttcg gcctggggaa tgtttggcag gtctggggcg
     5461 actggcggcg cggcgcggaa ctgctcgaag cgagcctggc gctctcgcag ggccgcggcg
     5521 gccactgggg cttcgtgcgt ggtctgccgc tggtcatgct cggctggctg ttgctggagc
     5581 agggcgagca ggcgcgcgca ctgcccttgc tgcaggaggc cctggccctc ggcacgcgcc
     5641 tgggtgatca agtggcaagc ggcaccgcgc tcatcggcct gggctgggcc gcacacttcg
     5701 ctggggatga ccgccgcgcg ctggcgctgc tggaggaggc gctggggctc ttccgggcgc
     5761 tggggcatca ccaggggcgc ctcgacgcgc tggccagcct cggctgggtg agtctccagg
     5821 gcgaggacga cgagcgcgcc gccgcgctct tccgtgaggc gctgaccctc gccgacgaga
     5881 gcggctaccg ggcctatgcc ggtgactgcc tgcgtggcct cggtgggctg gccgggctcc
     5941 ggggagaggc gcggctggcg gcggagctgt tcggcgcggc cgaaggcctg cgcgagcgtg
     6001 ccggcacgcc gccctaccct tcgggcgccg cctaccggcg cgcggcagcc gcggtgcgtg
     6061 ccgagctggg tgacgcggcc tgggaagagg ccacggcggc ggggcgatcc cgaccgatcc
     6121 ccgagaccat cgccgcggca ctggccgccg ctacgccggc cagcggagga cgttgattca
     6181 ggcctcgtcg tccgctccct cgacggcgaa acgccgcgtg cgccagagca tcatcgggca
     6241 gtcatgctgg ttcttcggac cacggaagcc cttgagcgcc gggaagctgc cggtcccggc
     6301 tcagcgccac cgtcaccgtc accgtcaccg tcacgtctgc acgctgaacg gcgcgtcccg
     6361 gctacccggc atcagtcgcg ccagccagcg atgcgccggc cgcgctcgcg ccggcacgac
     6421 ggagatgtct tccgttccca cgatcgagcg atcgccgagc ggatcggact gggagcttgc
     6481 ctgaccttcg tcca
//
LOCUS       DASUBI010000733         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1095878, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000733 DASUBI010000000
VERSION     DASUBI010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095878"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFU72_14325"
     CDS             complement(<1..1163)
                     /locus_tag="VFU72_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5424717.1"
                     /translation="MTAPQARPYGEWRSPITTESIVAGSIRLALPMVAASGLYWLERR
                     AQEGGRMVVVRRAPDGRIADVTPPDFNARTRVHEYGGGSYVVHGDVVYFSNFTDQRLY
                     RQGLGGSPAPITPEPEVPAGLRYADARVTPDGATIVCVREWHRRDGRVDNELVSLPTD
                     GSAEPRVIASGHDFYSSPRLSPDGTRLAWLSWDHPNMPWDEAVLSVAEFAPPGELRDV
                     RQVAGGSGESIFQPEWAPDGTLYFVSDRTGWWNLYRERGGQAEGLAPLEAEFGEPQWA
                     FGMSRYGFLDDGELAAIYTQGGFDHLGVIEPDSRRLHPMATPYSALSGLVSDGKRLHL
                     IAGSATEPAALIAVEPRSGAVEVIRRSSSVEIAPGYISEPEALDFPTGEGLTA"
     gene            complement(1191..>2102)
                     /locus_tag="VFU72_14330"
     CDS             complement(1191..>2102)
                     /locus_tag="VFU72_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5424718.1"
                     /translation="ASDATLLAVFERLADTDIPYGLHAENDTLLQAGINQMQAEGRCD
                     PMAHALSRPPVVELEAINRALFFADYTGGYAYICHVTTAESFAMIEDARARGVRVRGE
                     TCPQYLLLSEEDLEKLGPFARCAPPLRSLEDVEGLWSYVADGVVDVISSDHCGYTIES
                     KEAGRDDIFKAPLGLSGIQTMFPAVLDAMLNDRGLEIEDFVRLTAGNPAEIFSLYPRK
                     GALLVGSDADIAIYDPHASWTAHGADMLHRNKWTPMEGREINARVVRTIIRGETVYRW
                     ENGHQVLGHKGFGKFLRRGYGHSQDVL"
BASE COUNT          310 a          734 c          738 g          320 t
ORIGIN      
        1 tgcgcggtca ggccctctcc ggtcgggaag tccagcgcct ccggctccga gatgtagccg
       61 ggcgcgatct cgacgctact gctccgccgg atgacctcga ccgcaccgct ccgcggctcg
      121 accgcgatca gcgcggccgg ctcagtggcg ctgcccgcga tcaggtgcag ccgctttccg
      181 tcgctgacca ggcccgagag cgcgctgtag ggggtcgcca tcggatgcag ccggcggctg
      241 tcgggctcga tcacgcccag gtggtcgaag ccgccctggg tgtagatcgc ggcaagctca
      301 ccatcgtcca ggaagccgta gcgcgacatg ccgaaggccc actgcggctc gccgaactcg
      361 gcctccagcg gcgccagtcc ctcggcctgc ccaccacgct cgcggtagag gttccaccag
      421 ccggtgcggt cggagacgaa gtagagcgtg ccgtccggcg cccactccgg ttgaaagatc
      481 gactcacccg agccaccggc gacctgccgc acgtcgcgca gctcaccggg cggcgcgaac
      541 tccgccaccg agaggacggc ctcatcccag ggcatgttgg gatggtccca gctgagccag
      601 gcgagccgcg tgccgtcggg cgagaggcgc ggcgacgagt agaagtcgtg accgctcgcg
      661 atgacgcgcg gctccgcgga tccgtcggtc ggcagggata ccagctcgtt gtcgacgcgg
      721 ccatcgcggc ggtgccactc gcgaacgcag acgatcgtcg caccgtcggg cgtgacgcgg
      781 gcatcggcgt agcgcagccc ggccggcacc tccggctccg gcgtgatcgg cgccggcgag
      841 ccgcccagcc cctggcgata gagccgctgg tcagtgaaat tcgagaaata caccacgtca
      901 ccgtggacga cgtagctgcc gccgccgtac tcgtggaccc gcgtgcgcgc gttgaagtcg
      961 gggggcgtga cgtccgcgat gcgaccgtcg ggggcgcggc gcacgactac catgcgcccg
     1021 ccctcctggg cgcgcctctc cagccagtag aggccgctgg cagcgaccat tggcagcgcc
     1081 agccggatcg agccggccac gatcgactcg gtagtgatcg gcgagcgcca ctcgccgtac
     1141 ggccgcgcct ggggtgccgt catggttgtt cctctttccc agctcgccgc ttagagcaca
     1201 tcctgcgagt gaccgtagcc gcggcgcagg aacttgccga agcccttgtg gccgagtacc
     1261 tggtgaccgt tctcccagcg gtagacggtc tcgccccgga tgatggtgcg caccacccgc
     1321 gcgttgatct cgcgcccctc catcggcgtc cacttgttgc ggtgcagcat gtcagcgccg
     1381 tgggcggtcc aggaggcgtg cgggtcgtag atggcgatat cggcgtcgga gccgaccagc
     1441 agcgcgccct tgcgcgggta gagcgagaag atctcggccg ggttgccggc ggtcagacgc
     1501 acgaagtcct cgatctccag cccgcggtcg ttgagcatgg cgtcgagcac ggccgggaac
     1561 atcgtctgga tgcccgagag gccgagcggc gccttgaaga tgtcgtcgcg ccccgcctcc
     1621 ttgctctcga tggtgtagcc gcagtggtcg gaggagatga cgtcgactac cccgtccgcc
     1681 acgtagctcc acaggccttc gacatcttcc agcgagcgca gcggcggcgc gcagcgggcg
     1741 aaggggccga gcttctccag gtcttcctcg ctcagcagca ggtattgcgg gcaggtctcg
     1801 ccgcgcacac gcaccccgcg cgcccgcgcg tcctcgatca tggcgaagct ctcggcggtc
     1861 gtgacgtggc agatgtaggc gtagccaccg gtgtagtcgg cgaagaacag cgcgcggttg
     1921 atcgcctcca gctcgaccac cggcggacgc gagagcgcgt gcgccatggg atcgcagcgt
     1981 ccttcggcct gcatctggtt gatgcccgcc tgcaagagcg tgtcgttctc ggcgtgcagc
     2041 ccgtagggga tgtcggtgtc ggccaggcgc tcgaagaccg ccagcagggt ggcgtcgctg
     2101 gc
//
LOCUS       DASUBI010000734         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1182481, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000734 DASUBI010000000
VERSION     DASUBI010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182481"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFU72_14335"
     CDS             complement(<1..606)
                     /locus_tag="VFU72_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5424719.1"
                     /translation="MSEQPTIVAVYAHPDDEVFSAGGTLAKYARTCRVVLVTATRGEL
                     GEIRVPGSATPETLPEVRERELRESAHILGVHEVRFLDYRDSGMAGTPGNQDARSLLQ
                     ADRTQATGRLVALFRELRPRAVLTFEETGGYGHPDHITIHELTTAAFDAAGDALRGAR
                     LFYSGFPRGLMRAFQEEARAAGVDLGGLGELDIERFGLPDDA"
     gene            complement(618..1418)
                     /locus_tag="VFU72_14340"
     CDS             complement(618..1418)
                     /locus_tag="VFU72_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5424720.1"
                     /translation="MTITSRSNTTIKAIRRLRARGERERAGRFFVEGVRQVAEAVQLG
                     APVEELIVAPELLRSPFARELVTGAAGRGTPVLEVSSEVFGSLSLKEHPQGLGAVVRQ
                     RWAALDEIDPRAGLCWVALESAADPGNLGTILRTAEAVGAGGVILLGQSTDPYDPTAV
                     RASTGAVFSQQLVRATLPAFAAWAARHGLQIAGTSPDTALDYAGAPYRRPLVLLLGSE
                     RQGLSEDALAVCDLLVRIPMAGRSDSLNLAVATGIVLYEIFNQSRSAT"
     gene            1565..2485
                     /locus_tag="VFU72_14345"
     CDS             1565..2485
                     /locus_tag="VFU72_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5424721.1"
                     /translation="MKVGVIMPLGESQAIGRPPSYAELRELALQAEESGFDSVWVYDH
                     LIYRFPERPQFGIWECWSILAGLAEATKRVELGTLVMCALWRNPALLAKMAVTTDEVS
                     GGRLILGIGAGWHEPEFTAFGYPYDHLAGRFSEALRIIAPLLKEGKVDFHGKYFSAPE
                     CELTPRGPRPGGPPLLVASKGQQMMRLTAKYADAWNTAWYGRASAIATRRAEMEQACR
                     DVGRDPNSLEVTVGVNVTFEQPSGTPTTPIDPDRTLTGTANDIAAALRDYAAAGVGHV
                     IIGLPLTQAPSDTARGLDVLGKALHTFKQA"
     gene            complement(2535..2906)
                     /locus_tag="VFU72_14350"
     CDS             complement(2535..2906)
                     /locus_tag="VFU72_14350"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEU5424722.1"
                     /translation="MGNTLVVLVYLAIIVVEIAASWIMFQKAGQPGWASIIPIYNVVV
                     MLRMIGRSGWWTLLAFVPIADILLLVWVASGLAKAFGKGTGFAVGLFFLNFIFIPILA
                     FGDAQYLGPNGTGSARIAPAI"
BASE COUNT          644 a         1275 c         1246 g          545 t
ORIGIN      
        1 cgcgtcgtcg ggcaggccga agcgctcgat gtccagctcg cccagcccgc cgagatcgac
       61 tccggccgcc cgggcctcct cctggaaggc gcgcatcagc ccgcgcggga agccagagta
      121 aaagagacgc gccccgcgca gcgcgtcgcc ggccgcgtcg aaggcggcgg tcgtcaactc
      181 gtggatggtg atgtggtcgg ggtggccgta gccgccggtc tcctcgaagg tcagcacggc
      241 ccgcggccgc agctcgcgga agagcgccac caggcggccg gtggcctggg tgcggtcagc
      301 ttgcagcagc gagcgagcgt cctggttgcc gggcgtgccg gccatgccgg agtcgcggta
      361 gtcgaggaaa cgcacctcgt ggacgcccag aatgtgggcc gactcccgca gctcccgctc
      421 gcgtacctcg ggcagcgtct ccggcgtggc gctgccgggc acgcgaatct cgcccagctc
      481 gccgcgcgtg gccgtcacca gcaccacgcg gcaggtgcgg gcgtacttgg ccagcgtgcc
      541 gccggcgctg aagacctcgt cgtcggggtg cgcgtagacg gccacgatgg tcggctgctc
      601 gctcacggat gctcctctca tgtcgcggaa cggctctgat tgaagatctc gtagaggacg
      661 attccggtcg ccaccgccag attcagcgag tcgctgcggc cggccatggg gatccggacc
      721 agcaggtcgc agacagccag cgcgtcttcg gatagtccct ggcgttcgct ccccagcagc
      781 agaaccagcg ggcgccggta gggcgcgccg gcgtaatcca gcgcggtatc cggcgaagtc
      841 ccagcgatct ggagcccatg acgcgcggcc caggcggcga atgcagggag cgtggcccgc
      901 accagttgct gcgagaagac cgccccggtg ctggcgcgca cggcggtcgg atcgtatgga
      961 tcggtgctct gcccgagcag gatgaccccc ccggcgccga ccgcctcggc ggtgcgcaaa
     1021 atggtgccga ggttgccggg atcggccgcc gactccagcg ccacccagca gagcccggcg
     1081 cgcgggtcga tctcgtccag cgccgcccag cgctggcgca ccaccgcgcc cagcccctgg
     1141 gggtgctcct tgagcgacag gctcccgaag acttcggagc tgacctccag caccggtgtg
     1201 ccccggccgg cggcgcccgt caccagctcg cgggcgaagg ggctgcgtag cagctccggc
     1261 gccacgatca gctcctcgac cggcgccccg agctggaccg cctcggccac ctggcgcacg
     1321 ccctcgacga agaagcggcc ggcgcgctcg cgctcgccgc gcgcgcgcag cctgcggata
     1381 gccttgatcg tggtgttgct tcggctggtg atcgtcatcg ccgcgcgccc acctccgcac
     1441 gccagtctac gcgatcggcc gatcccagcg cacacggggg tggtccattg gtacagtgcc
     1501 gaaacacgct ccgacgggta tgctgtcgcg gcccaatctg gggcggggag cgaagggaaa
     1561 ccgcatgaaa gtcggcgtca tcatgccgct gggcgagagt caggcgatcg gccggccacc
     1621 atcctacgcg gagctgcgcg agctggcgct ccaggccgaa gagtccggct tcgactcggt
     1681 gtgggtctac gaccacctga tctaccgctt cccggagcgg ccgcagttcg gcatctggga
     1741 atgctggtcg atcctggccg ggctggccga ggcgaccaaa cgggtcgagc tggggacgct
     1801 ggtgatgtgc gcgctctggc gcaacccggc actgctggcc aagatggcgg tcacgaccga
     1861 cgaggtcagc ggcgggcggc tgatcctggg catcggcgcc ggctggcacg agccggagtt
     1921 caccgccttc ggatacccct atgaccacct ggccggccgc ttctcggaag cgctgcggat
     1981 catcgccccg ttgctgaagg aggggaaggt cgactttcac ggcaagtact tcagcgcgcc
     2041 cgagtgcgag ctgaccccgc gcggcccgcg cccgggtggt ccgccgctgc tggtggccag
     2101 caaggggcag cagatgatgc gcctgacggc caaatacgcc gacgcctgga acaccgcctg
     2161 gtacgggcgc gccagcgcga tcgccacgcg ccgggcggag atggagcagg cttgccggga
     2221 tgtggggcgc gacccgaact cgctggaggt cacggtcggt gtcaacgtca ccttcgagca
     2281 gccgagcggc acaccgacca cgccgatcga tcccgaccgg accctcaccg gcacggccaa
     2341 cgacatcgcg gcggcgctgc gcgactacgc cgcggccggg gtcggccacg tcatcatcgg
     2401 cctgccgctc actcaggcgc ccagcgacac cgccaggggg ctcgacgtgc tgggcaaggc
     2461 cctgcacacg ttcaagcagg catagcgcga cggggccggc atgatcgccg gccccgtttg
     2521 tcacgtctcg atggctagat cgccggcgcg atgcgcgcgc tgccggtgcc gttcggtccg
     2581 aggtactgcg cgtcgccgaa ggccaggatc gggatgaaga tgaagttcag gaagaatagc
     2641 cccaccgcga acccggtgcc cttgccgaac gccttggcca ggccggaggc gacccagacc
     2701 agcagcagaa tatccgcgat tggcacgaac gccagcagcg tccaccagcc ggagcggccg
     2761 atcatgcgca gcatgacgac cacgttgtag atcgggatga tcgacgccca gccgggctga
     2821 ccggccttct ggaacatgat ccagctcgcg gcgatctcga ccacgatgat cgccagatag
     2881 accaagacaa cgagagtatt gcccacccac agcctccttc ggcgtacgct cacgccacca
     2941 gtcaaggaac cgccgcgcca atacatgtgt acgtactatc tatatacttc accccagacc
     3001 gcccggtaat agtacggggg tcctgcttac gcatcaccgc gcgcgcggga atagcgaccg
     3061 cgcaacgcgc cggccatcat tggcaattcc taattgatcg tgaaccgcgc gaccggtccg
     3121 ttcggggccg gatggcgccg acggtataat tgagggaatc ggggagagca acggtcggct
     3181 gggcttcccc gccgagtggg cggcccgatc atcggcccgc ccacgagcat cgcccccgta
     3241 gttcagtgga cagaacggcg gccgtctaga gcgtgttagg cactcgtttg aaacccgcgt
     3301 cgcgacgcgg aatcgtcagc cgcatcagat ctgcggaatc cgcgtcggca agccctttct
     3361 gggcaagtgc ctaccagcct ctatgccgct gaggggatcc cgactgccaa cgctgccaag
     3421 gggatagcca gttttgcagt ccgccacgtc ccagcgtctg agtcactggg aacgagactt
     3481 tcgagagcac tggcgttgca ccacccaaga atcgaggtgc tagatggcga cctatatcta
     3541 caaacgataa gcgcccagcg ccggtcgcgc ctaccaacct caagttcatt tctagtgaga
     3601 tagaagctca catcctcact ggaagctctt gcagaggtct ttacctcaaa atgacgtgga
     3661 cttcccacga tagaaggagc gaagacgtca taaccaaagc tatctgaaat
//
LOCUS       DASUBI010000735         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_752736, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000735 DASUBI010000000
VERSION     DASUBI010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752736"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFU72_14355"
     CDS             <1..551
                     /locus_tag="VFU72_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424723.1"
                     /translation="AISPAVYIILFPLSFRLTCYYYRLAYYRAFFWDPPACAVQELAA
                     RKHYTGERQFPFVLQNIHRFAFYIAVIFIFILSYDAIRAFFFNGQFGIAVGSLIFLIN
                     VVLLALYTFGCHSFRHLIGGRINCYSCTLRNRTRYSIWQRVSQLNERHGLFAWASLIA
                     VGLTDLYVRLVASGAIHDVRFF"
     gene            555..2351
                     /locus_tag="VFU72_14360"
     CDS             555..2351
                     /locus_tag="VFU72_14360"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5424724.1"
                     /translation="MEAYDTFEHDILIIGAGGAGLRAAIAAGAAGMSVGVVCKSLLGK
                     AHTVMAEGGIAGALANLDKKDSWQVHFQDTMRGGAFLNNWRTAEILAKEAPERVLELE
                     QWGAVFDRTPEGLIMQRPFGGHTYRRLNHVGDRTGLELIRTLQDRAVATGMTCYMEVT
                     MTRLLKDGDRVVGAFGYYRETGRFLAIKARAVVLATGGWGRMFKVTSNSWESTGDGAM
                     LAFDAGAELMGMEMVQFHPTGMVWPPGVRGLLVTEGVRGEGGILRNSKGERFMFDPKY
                     TPASYKGRYADTEEEAGRWIEDKANNRPPPELLPRDVVARAIYREVLAGNGTEHGGVY
                     LDVTHRGADFIRRKLPSMYEQFHALGDVDITAQPMEVYPTIHYTMGGVRAEPETAATT
                     LPGLYAAGEVSAGLHGANRLGGNALCELLVFGKRAGEGAAQYAQSAGPGTLDEAAIQE
                     EAASLLAPFESSGTENPYVITEELQAAMQEGAMIARTEESLQKCLNTVLELQQRAHNL
                     HVEGSRVYNPGWHTARDVHNMLKISEIIVRCALERQESRGAQWRLDYPNPDPEWAKKN
                     LIATRQDDTVRITTTPVPAMPPELAGLFEEGH"
     gene            2352..3101
                     /locus_tag="VFU72_14365"
     CDS             2352..3101
                     /locus_tag="VFU72_14365"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU5424725.1"
                     /translation="MYMVTLRVFRGDASGGTERDYQVPVFPGMVVLDAIHYIQAHNDS
                     SLACRWNCKAGKCGSCSAEVNGRPRLMCKTRIADYTSTDAPIVVHPLKTFPLIKDLVT
                     DVSWNYRMSAKIPPFTPAADAPRPLVMQQIDVDRIQEFRKCIECFLCQDVCHVLRDHH
                     KHEDFIGPRFFVYSAALEMHPLDTEDRVPDLRREHGIGYCNITKCCTKVCPEHITITD
                     NAIIPLKERVVDEFFDPIGKLVKIYSRRKRS"
     gene            3137..>3349
                     /locus_tag="VFU72_14370"
     CDS             3137..>3349
                     /locus_tag="VFU72_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424726.1"
                     /translation="MNLKPITREGIPAALQKAERYRLLNESSAAESICLDVLDIDPTN
                     QEALVSLLLAITDQFGEEMSDGVRRAR"
BASE COUNT          597 a         1133 c         1081 g          538 t
ORIGIN      
        1 gggcgatctc cccggcggtc tacatcatcc tgttcccgct cagcttccgg ctgacctgct
       61 actactaccg gctggcctac taccgggcgt tcttctggga cccgcccgcg tgcgccgtcc
      121 aggaactggc cgcccgcaag cattacacag gcgagcgcca gttccccttt gtgctgcaaa
      181 acatccaccg cttcgccttc tacatcgcgg tgatcttcat cttcatcctg tcctacgacg
      241 ccatccgcgc gttcttcttc aacggccagt tcggtatcgc ggtggggtcg ctcatcttcc
      301 tgatcaacgt ggtgctgctg gcgttgtaca ccttcgggtg ccactcgttc cgccacctca
      361 tcggcgggcg gatcaattgc tactcctgta ccctgcgcaa ccgcacgcgc tacagcatct
      421 ggcagcgcgt cagtcaactg aacgagcgtc atggcctgtt cgcctgggcc agcctgatcg
      481 cggtcggcct gaccgacctc tatgtgcggc tggtggcttc gggcgccatt cacgatgtga
      541 ggttctttta gcatatggag gcttacgata cctttgagca cgacatcctg ataatcggcg
      601 cgggcggggc gggcctgcgc gccgccatcg cggcgggcgc tgctgggatg agcgtcggcg
      661 tggtctgcaa gtcattgctc ggcaaggcgc acaccgtcat ggccgagggc ggcatcgccg
      721 gggcgctggc gaacctcgac aaaaaggact cctggcaggt ccacttccag gatacgatgc
      781 gcggcggcgc gttcctcaac aactggcgca cggcggagat cctggcgaaa gaggcccccg
      841 agcgggtgct ggagctggag cagtggggcg cggtgttcga ccgcacgccc gagggcctga
      901 tcatgcagcg gcccttcggc ggccacacct accgccgcct caaccacgtc ggcgaccgca
      961 ccgggctgga actgatccgc accctgcagg atcgcgccgt cgccacgggg atgacctgct
     1021 acatggaagt gaccatgacc cggctgctca aggacggcga ccgggtggtg ggcgcgttcg
     1081 gctattaccg cgagacgggg cgcttcctcg ccatcaaggc gcgggcggtg gtgctggcga
     1141 ccggcggctg ggggcgcatg ttcaaagtga cctccaactc ctgggagtcc accggcgacg
     1201 gcgccatgct ggccttcgac gccggagccg aactgatggg catggagatg gtgcagttcc
     1261 accccaccgg catggtctgg ccgcccggcg tgcgcggcct gctcgtcacc gagggcgtgc
     1321 gcggcgaggg cggcatcctg cgcaacagca agggcgagcg cttcatgttc gaccccaagt
     1381 acacgcccgc ctcctacaaa gggcgctacg ccgacaccga ggaggaggca ggccgctgga
     1441 tcgaagacaa ggcaaacaac cggcccccgc ccgagttgct gccgcgcgac gtggtggcgc
     1501 gcgcgatcta ccgcgaggtg ctggccggca acggcaccga gcatggcggc gtctacctcg
     1561 acgtgaccca tcgcggcgcg gacttcatcc gccgcaagct gccgagcatg tatgagcagt
     1621 ttcacgcgct gggcgacgtg gacatcacgg cccagccgat ggaagtctac ccgacgatcc
     1681 actacacgat gggcggggtg cgcgccgagc ccgaaacggc ggcgacgacg ctgcccggcc
     1741 tctacgcggc gggcgaggtg tctgccgggt tgcatggtgc caaccgcctg ggcggcaacg
     1801 ccctgtgcga actgctggtg ttcggcaagc gcgcgggcga gggcgcggcc cagtacgcgc
     1861 aatccgccgg ccccggcacg ctcgacgaag ccgccatcca ggaggaagcg gcctcgctgc
     1921 tggccccctt cgagtcatcg ggcaccgaga acccctacgt gatcaccgag gagttgcagg
     1981 cggccatgca ggagggggcg atgatcgcgc gcaccgagga gagcttgcaa aagtgcctca
     2041 acacggtgct cgaactccag cagcgggcgc acaacctgca tgtcgagggc tcgcgcgtct
     2101 acaaccccgg ctggcacacg gcgcgcgacg tgcacaacat gctcaagatc tcggagatca
     2161 tcgtgcgctg tgcgctggaa cgccaggaaa gccgaggcgc gcaatggcgg ctcgactatc
     2221 ccaaccccga cccggaatgg gccaaaaaga acctgatcgc cacccggcaa gacgacaccg
     2281 tccggatcac gacgaccccc gtcccggcga tgccgcccga actcgccggg ctgttcgagg
     2341 agggccacta aatgtacatg gtcaccctgc gggtgttccg cggcgacgca tccggcggca
     2401 ccgagcgcga ctaccaggtg ccggtgtttc cgggcatggt ggtgctcgac gcgatccact
     2461 acatccaggc gcacaacgac agcagcctcg cctgccgctg gaactgcaag gcgggcaaat
     2521 gcggctcgtg cagcgccgaa gtcaacgggc gcccgcgcct gatgtgcaag acccgcatcg
     2581 ccgattacac cagcaccgac gcgccgattg tggtccaccc gctgaagacg ttcccgctga
     2641 tcaaagacct ggtgacggac gtgtcctgga actaccgcat gtcggccaag atcccgccgt
     2701 tcacgcccgc cgccgacgcc ccgcgcccgc tggtcatgca gcagatcgac gtggaccgca
     2761 tccaggagtt ccgcaagtgc atcgagtgct tcctgtgcca ggacgtgtgc cacgtgctgc
     2821 gtgaccacca caagcacgag gacttcatcg ggccgcgctt tttcgtctac agcgcggcac
     2881 tcgagatgca cccgctcgat accgaggatc gcgttcccga tcttcgccgc gagcacggca
     2941 tcggctactg caacatcacg aagtgctgca ccaaggtgtg ccccgagcac atcacgatca
     3001 ccgacaacgc gatcattccg ctcaaggagc gcgtggtcga tgagttcttc gatccgatcg
     3061 ggaagctggt gaagatctac agccggcgaa aacggagctg atccgatttc ggcgcggctg
     3121 acacgcggag cgtagcatga acctcaaacc gatcacgcgc gagggtattc cggccgcgct
     3181 gcagaaggcg gaacgctatc gtctgctcaa cgaatcgtcg gcggcggaga gcatctgcct
     3241 cgacgtgctg gacatcgatc cgacgaatca ggaggcgctg gtttcgctgc tcctggcgat
     3301 cacggaccaa ttcggcgagg agatgtccga cggcgttcgt cgcgcgcgc
//
LOCUS       DASUBI010000736         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_509915, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000736 DASUBI010000000
VERSION     DASUBI010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509915"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2203
                     /locus_tag="VFU72_14375"
     CDS             <1..2203
                     /locus_tag="VFU72_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5424727.1"
                     /translation="LRCEWLGVFDALRLRLEATRADALLGGGRYERAAACLSGPIAAW
                     PLHEPLYERLLVAYYCAGRRADALGVYRTLRATLREGLGADPAERMRTLHGLVLRDAA
                     VEEVLGTWRWWQGADTPVTASVTLAAPSSPASTPVACQLPRRPHLLLGRDAELASLTA
                     MLTAERAGDSRLAVLTGPAGVGKTSLAVAWAHSAAEHFPDGRFYLDLNGFCADASPLT
                     ADDAVAILLDFLGVPAHDRPATAEGRLARYRAATAGKRLLMVFDNVCDAAQARRLLPS
                     GDACRALVTSRRRLTGLVALEGAQQIALTALAAEDSRALLARRLGSRRVSGQEAAVEA
                     IADRCAHLPLALSVAAARAAAMPGTPLTALADQLHAGALDALSAGDAASDVRNVLSWS
                     YLRLPEAAARVFRFLGLHPGPEITAPAAAALADVPRSEAVALLADLGAMNLLAEPEPG
                     RYTMHSLLRAFAVERFTAVAAPTARRAALHRLYDSSLRWAIEAERAFATVPTTARADN
                     VRLPPGLEPPRFAAEDALAWFDAERAVLAALIAHAASDGLDDYACRLAAAHSGALMAR
                     RHYPDAARQGRIALSVARRLGDLGGQAGALFQIGRALSHSGESEIARYHLERALRIQR
                     SLGDDTGLTETHRALAETHRDLGNVPRWRHHTMRYLASATRIGMRGPAPGRVVSGTAT
                     ATATATATATATATATATATATATASGQLVSAAPASASASEQRESRPAHRS"
     gene            2296..3789
                     /locus_tag="VFU72_14380"
     CDS             2296..3789
                     /locus_tag="VFU72_14380"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEU5424728.1"
                     /translation="MTHLKSLREFLSALEEIGDLKRIDKEVDWNLEVGAIIRRSYDLK
                     APAPLFTNLTGYGESGFRLFGAPGALSASGHPFARIALALGLPAEATGQQIVAALADA
                     RGKPGIPPVEVERSQAPCKQNVMVGDDIDLFAFPTPLVHGNDGGRYIQTYGMNIAKTP
                     DGSWTNWSINRMMIHDRNTLACLIPAPQHLGVIRQQWIERGEPMPIALAIGVEPGLPW
                     VGGMPIPEGADETHYLGALFGEGIELVRAETVDLMVPATAEIVIEGHIALPDETVMEG
                     PFNEFPGYNATDATPKAVFHVSAITYRDGAIQPVVAAGPPVEEDHTIIGTTSAAEILY
                     LLREAGLPVSSAWYNFEAAVHWLTLAVRQDWHENTGLGSHELIDAIAGVIFHGKPSVN
                     APKILVVEDDVDITDLSEVVWAFATRSHPDVERGEFHYPPAVSDQLAVYLTQEESRAF
                     MAGKVIYNCLLADLYPAGKRPVKGSFENGWPSDIQQRVLANWHEYGY"
BASE COUNT          534 a         1391 c         1406 g          565 t
ORIGIN      
        1 cctgcgctgc gaatggctcg gcgtcttcga cgccctgcgc ctgcgcctgg aggcgacgcg
       61 cgccgacgcg ctgctgggcg gcggacgcta cgagcgcgcg gcggcgtgcc tgtcggggcc
      121 gatcgccgcg tggccgctgc acgagccgct gtatgagcgg ctgctggtgg cgtactactg
      181 cgcgggacgc agagctgacg cgctcggcgt ctaccgaacg ctgcgcgcca cgctgcgcga
      241 agggctcggc gcggatccgg ccgagcggat gcggacgctg cacggcctgg tgctgcggga
      301 cgcggcggtc gaggaggtgc tggggacgtg gcggtggtgg cagggggctg atacgccggt
      361 gacggcatca gtcacactgg cagccccctc atctccggcc tcgacaccgg tcgcctgcca
      421 actcccccga cgcccacacc ttctgctcgg ccgcgacgcc gaactggcct cgctcacggc
      481 gatgctcacc gccgagcggg ccggcgacag ccgcctcgcc gtcctgaccg gcccggccgg
      541 cgtcggcaag acctcgctcg ccgtggcctg ggcccacagc gccgccgagc acttccccga
      601 cggccggttc tacctcgatc tcaacgggtt ctgcgccgac gcctcgccgc tgaccgccga
      661 cgacgcggtc gccatcctgc tggacttcct cggcgtgccc gcccacgacc ggcccgcgac
      721 cgccgagggc cgactggcgc gctaccgggc cgcgaccgcc ggcaagcggc tgctgatggt
      781 cttcgacaac gtctgcgacg ccgcgcaggc ccggcggctg ctgccgtccg gcgacgcctg
      841 ccgggccctg gtcaccagcc gccgacggct gaccggcctg gtcgcgttgg agggcgcgca
      901 gcagatcgcg ctgaccgccc tggccgccga ggactcgcgc gcgctgctgg cccggcgtct
      961 cggctcccgg cgcgtcagcg ggcaggaggc cgccgtcgag gccatcgccg accggtgcgc
     1021 ccatctgccg ctggcgctgt cggtcgccgc ggctcgcgcc gccgcgatgc ccggcacgcc
     1081 gctcaccgcg ctggccgacc agctgcacgc cggcgcgctc gacgcgttga gcgccggcga
     1141 cgcggcgagc gacgtgcgta acgtgctgtc ctggtcgtat ctgcggctgc cggaggcggc
     1201 ggcgcgtgtg ttccgcttcc tcggcctgca tccgggtcct gagatcaccg cgccggccgc
     1261 cgccgcgctg gccgacgtac cgcgctccga ggcggtggcg ctgctggccg acctcggggc
     1321 gatgaacctg ctcgccgagc ccgagccggg ccgctacacg atgcacagtc tgctgcgcgc
     1381 cttcgccgtc gagcggttca cggccgtggc ggcgccgacc gcccggcgcg ccgcgctcca
     1441 ccggctctac gactcctccc tgcgctgggc gatcgaggcc gagcgggctt ttgccaccgt
     1501 gccgaccacg gcccgcgccg acaacgtgcg tctgccgccc ggcctggagc cgccacggtt
     1561 cgcggccgag gacgcgctgg cctggttcga cgccgaacgc gcggtgctgg cggccctgat
     1621 cgcgcacgca gcgtccgacg gcctggacga ctacgcctgc cggctggccg ccgcgcactc
     1681 cggcgcgttg atggcgcgca ggcactatcc ggacgcggcg cgccaaggcc ggatcgcgct
     1741 gtccgtggcg cgccggctcg gcgatctggg cgggcaggca ggcgcgctgt tccagatcgg
     1801 cagggccctg agtcacagcg gcgagtccga gatcgcgcgc taccacctgg agcgcgcgct
     1861 gcggatccag cggtcgctcg gcgacgacac cggcctgacc gagacccacc gcgccctggc
     1921 cgagacgcac cgggacctgg gcaacgtccc gcgctggcgg caccacacga tgcgctatct
     1981 ggcctcggcg acgcggatcg gcatgcgggg cccggcgccg gggcgggtgg tgtcgggtac
     2041 ggcgacggcg acggcgacgg cgacggcgac ggcgacggcg acggcgacag cgacggcgac
     2101 agcgacagcg acagcgacag catcggggca gctcgtatcg gcggcgccgg cttcggcttc
     2161 ggcgtccgag cagcgtgaga gcaggccggc tcaccggtcc tgacctgcga catatcgcaa
     2221 cgcggcggtg ccgggatatc ggcacggtat agccggtctc taccttcctc gcgaacaccc
     2281 gaggaaggaa aaccagtgac gcatctgaag agtctgcgcg agttcttgtc cgcgttggaa
     2341 gagatcggcg acctgaagcg catcgacaag gaggtggact ggaatctgga ggtcggggcg
     2401 atcatccggc gctcgtatga cctgaaggct ccggcgccgt tgttcacgaa cctcaccggc
     2461 tacggcgagt ccggtttccg gttgttcggc gcgcctggtg cgctgtcggc gtcggggcat
     2521 ccctttgccc gtatcgcgtt ggcgttgggg ctgcctgccg aggcgaccgg tcagcagatc
     2581 gtggcggcgt tggctgatgc tcggggcaag cctgggatcc cgccggtgga ggtggagcgg
     2641 tcgcaggcgc cgtgcaagca gaatgtgatg gtcggcgacg atatcgatct gttcgcgttt
     2701 cccacgccgc tggtgcacgg caacgacggc ggccggtaca tccagaccta cggcatgaac
     2761 atcgccaaga ccccggacgg ttcctggacc aactggtcga tcaaccgcat gatgatccac
     2821 gaccggaaca ccctggcctg tctgatcccg gcgccgcagc atctgggcgt catccggcag
     2881 cagtggatcg agcggggcga gccgatgccg atcgcgttgg cgatcggggt ggagccgggc
     2941 ctgccgtggg tgggcgggat gccgattccc gagggtgccg atgagaccca ctacctgggc
     3001 gcgttgttcg gtgaggggat cgagctggtc cgggccgaga ccgtggacct gatggtcccg
     3061 gcgaccgcgg agatcgtgat cgagggccac atcgccctgc cggatgagac ggtgatggag
     3121 gggccgttca acgagttccc cggctacaac gccaccgacg cgaccccgaa ggccgtgttc
     3181 cacgtctcgg cgatcacgta tcgcgacggt gcgatccagc cggtggtggc ggccggtccg
     3241 ccggtggagg aggaccacac gatcatcggc accaccagcg ccgccgagat cctttacctg
     3301 ctgcgcgagg cgggccttcc ggtctcctcg gcctggtaca acttcgaggc ggcggtgcac
     3361 tggctcaccc tggctgtccg tcaggactgg catgagaaca ccggtctggg ctcgcacgag
     3421 ctgatcgacg cgatcgccgg tgtcatcttc cacggcaagc cctcggtgaa cgccccgaag
     3481 atcctggtgg tcgaggacga tgtggacatc accgacctgt ccgaggtggt gtgggcgttc
     3541 gcgacccgct cgcacccgga tgtcgagcgc ggcgagttcc actatccgcc ggcggtctcc
     3601 gaccagctcg cggtctacct cacgcaggag gaatctcgcg cgttcatggc cgggaaggtg
     3661 atctacaact gcctgctggc cgatctctac ccggccggca agcgcccggt gaagggcagc
     3721 ttcgagaacg gctggccgag cgacatccag cagcgcgtcc tcgccaactg gcacgaatac
     3781 ggctactgat ccacgtgcgc tgacctcacc cactgcgcgc cccgcgaata tggggcgcgc
     3841 agacatcgtt cgcggccgtg ggcggcagta ccttccgggc accccaacga tgaacc
//
LOCUS       DASUBI010000737         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1191526, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000737 DASUBI010000000
VERSION     DASUBI010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191526"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="fni"
                     /locus_tag="VFU72_14385"
     CDS             complement(<1..993)
                     /gene="fni"
                     /locus_tag="VFU72_14385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU5424729.1"
                     /translation="MSTDATPGPRTGSPTTPTRKADHLRINLEENVAAKGITTGFESY
                     RFIPRALPEIDLEQVDTGVRLFGRRLSAPLFISCMTGGTPEAARINRTLAQVAQQRRL
                     ALGLGSGRVLLEHPEVLPTFRVRPLAPDTLIFANLGAVQLNRGVSVDQCRRLVAMIEA
                     DALVLHLNALQEALQPEGDTCFGGLLERIADLCRRIEVPVIAKEVGWGIAPDVVSSLL
                     AAGVAAVDVAGAGGTSWSEVERHRSSDPVRRRVAAAFAGWGLPTAEAVRLARRAAPGR
                     LIFASGGIRDGMDVAKALALGADMVGIAGGFLRAAALGHDAALERAEVCVETLRT"
     gene            1215..2261
                     /gene="hpnH"
                     /locus_tag="VFU72_14390"
     CDS             1215..2261
                     /gene="hpnH"
                     /locus_tag="VFU72_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEU5424730.1"
                     /translation="MAITMRQNLTILTYILKQRLQGRDKYPLVLQLEPLYQCNLACAG
                     CGKIQHPTEVLRRRLSVRQCVDAVEECGAPVVSIAGGEPLVHQEIDQIVAALVERKRF
                     VYLCTNALLMERKLDRFTPSPYFSWSVHIDGLRSVHDASVQRDGVFDRAVSAIRAAQE
                     RGFRVTTNTTFFSQATPATVRDVLDFLNDELRVDAMQISPGYAYEKAPDQEHWLAVER
                     TRALFREAFGGGRRRRWRLNHTPLFLDFLEGKVDFACTAWGIPCYSVFGWQRPCYLMA
                     DGYAASYQELLETTDWASYGRGRNPKCGNCMAHCGYEPTAVMATMRSPRESLRATADI
                     VRGSLPLHVAHGHD"
     gene            2272..3255
                     /locus_tag="VFU72_14395"
     CDS             2272..3255
                     /locus_tag="VFU72_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5424731.1"
                     /translation="MSGATVFLTGATGFVGGHVARALLDAGYDVRALVRPGSRKPPEL
                     AGCDWVEGDLRRPGELARQIAGSRFLVHVAACYSFAPARRREIWDTNVSGTAGLLEAA
                     RLAGVERAVVTSSSATVGPARDGRPATEADHADPSGAVSVYHHSKIAQERAALASQLP
                     VVVVLPTTPVGPGDRRPTPTGKLIVDFMRGRMFGYVPGGMNLVPVEDVARAHVLALAR
                     GRPGERYLLGGDNLTFAEIWATLAELCGRPAPRLRVPYGLALTLGWADELRCRARPGQ
                     EPLVPLEGVAMSRHYMFASSDKAGAELGFAPGSSRAALARAVAWFREQPDA"
     gene            3248..3850
                     /locus_tag="VFU72_14400"
     CDS             3248..3850
                     /locus_tag="VFU72_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424732.1"
                     /translation="MPESGSPRVLAPTVVEYLAARLVLGRHAPRYAGVGLRRWQGTPG
                     DAPVVVCGLAGALTDDLPSGCVLIPENVALPGGPPRDCDPELVAAFTTAARSLGYEVR
                     SGPLVTVPVLASSEARARLTASGFAAADMETALLPDKLRIATVRVILDTPQRPLSARW
                     LKPTAALRPALWPELLWLARHAPAYAYRAGRVVRAGLGAV"
BASE COUNT          520 a         1466 c         1392 g          570 t
ORIGIN      
        1 ggtgcgcagc gtctcgacgc acacctcggc tcgctccagc gcggcgtcgt gtcccaaggc
       61 ggccgcgcgc aagaagcccc ccgcgatgcc gaccatgtcg gcgcccagcg ccagcgcctt
      121 ggcgacgtcc atgccgtcgc ggatgccgcc gctggcgaag atcaggcggc ccggcgcggc
      181 ccggcgcgcc agccgcaccg cctcggcggt cggcaggccc cagccggcga aggcggcggc
      241 cacccggcgg cgcaccggat cgctgctgcg gtgccgctcg acctcgctcc aggaggtgcc
      301 gcccgcgccg gccacgtcga cggccgccac gcccgccgcc agcaggctgc tcacgacatc
      361 cggcgcgatc ccccagccga cttccttggc gatcaccggc acctcgatgc gccggcagag
      421 gtcggcgatc ctctccagga ggccgccgaa acaggtgtca ccctcgggct gcagcgcctc
      481 ctgcagggcg ttgaggtgga gcaccagcgc atcggcctcg atcatcgcca ccaggcggcg
      541 gcactgatcg acgctgacgc cccggttgag ctgcaccgcg cccagattgg cgaagatcag
      601 cgtgtcgggc gcgagcgggc gaacgcgaaa cgtcggcaag acctcggggt gctccagcag
      661 cacccggccc gaaccgagcc caagcgccag gcgacgctgc tgggccacct gggccagggt
      721 gcggttgatg cgcgccgctt cgggggtgcc gccggtcatg caggagatga agagcggcgc
      781 gctcaggcgc cggccaaaga gccggacgcc ggtatcgacc tgctccaggt cgatctccgg
      841 cagcgcgcgt gggatgaaac ggtagctttc gaaaccggtg gtgatgccct tggcggcgac
      901 gttctcttcg agattgatgc gcaggtggtc ggccttgcgc gtcggtgtgg tcggtgagcc
      961 ggtcctgggg cccggagtcg cgtcggtgct catgaccaat cgcctccgtc tcgtgtgcct
     1021 gggccggcga gcgctgccgc gtcggtatgg acccgtcgca ccggccctac ggagtcctcc
     1081 gccgcaagcg gcggaccgca atacggcagc ttctctccgg ctcgccggga tgtcaaggtt
     1141 tcttgcgcgc cgcgttcgcg agcctataat cgaggcacgg gttgtttccc aggcaaccca
     1201 ggtaaggagg gcgcgtggca atcacgatgc gccagaatct cacgattctg acttacatcc
     1261 tcaaacaacg actccagggc cgcgacaagt acccgctggt gctccagctc gaaccgctct
     1321 accagtgcaa tctggcctgc gccggctgcg gcaagattca gcacccaacc gaggtgctcc
     1381 ggcggcgcct gtcggtgcgg cagtgcgtcg acgcggtcga ggagtgcggg gcgccggtcg
     1441 tctcgatcgc gggcggcgaa ccgctggtcc atcaggagat cgatcagatc gtggcggcgc
     1501 tggtcgagcg caagcgcttc gtctacctct gcaccaacgc gctcctgatg gagcgcaagc
     1561 tcgaccgctt caccccctcg ccctacttct cctggtcggt gcacatcgac ggcctgcgct
     1621 cggtccacga cgcctcggtg cagcgcgacg gggtcttcga ccgcgcggtg agcgccatcc
     1681 gcgccgccca ggagcgcggc ttccgggtga cgaccaacac caccttcttc tcgcaggcca
     1741 cccccgccac cgtccgggac gtgctggact tcctcaacga cgaactgcgg gtcgacgcca
     1801 tgcagatctc gcccggctat gcctacgaga aggcgcccga tcaggaacac tggctggcgg
     1861 tcgagcgcac ccgcgcgctc ttccgcgagg cgttcggcgg cgggcggcgg cgccgctggc
     1921 ggctcaacca caccccgctc ttcctcgatt ttctggaggg gaaggtcgat ttcgcctgca
     1981 ccgcctgggg catcccctgc tactcggtct tcggctggca gcgcccctgc tacctgatgg
     2041 ccgacggcta cgcggcgtcc tatcaggagc tactggagac gaccgactgg gcgtcctacg
     2101 ggcgcgggcg caacccgaag tgcggcaact gcatggccca ctgcggctac gagccgaccg
     2161 ccgtgatggc caccatgcgc tcgccgcgcg agtcgctgcg cgccacggcc gacatcgtgc
     2221 gcggcagcct cccgctccac gtcgcccacg gccacgactg acgccggagt catgagcggg
     2281 gcaaccgtct tcctcaccgg cgccaccggc ttcgtcggcg gccacgtggc gcgcgccctg
     2341 ctcgacgccg gctacgacgt ccgcgcgctg gtgcgaccgg ggagccgtaa gccacctgag
     2401 ctggcgggct gcgactgggt cgagggcgac ctgcgccggc ccggcgagct ggcgcggcag
     2461 atcgcgggca gccgatttct cgtccacgtg gccgcctgct actccttcgc gcccgcgcgc
     2521 cggcgtgaga tctgggacac gaacgtgtcc ggcaccgccg ggctgctgga ggcggcccgt
     2581 ctggccggcg tcgaacgcgc ggtggtcacc tccagctcgg ccacggtcgg cccggcgcgc
     2641 gacggccgcc cggcgaccga agccgatcat gccgatccct ccggcgccgt ctcggtctac
     2701 catcactcca agatcgcgca ggagcgcgcg gcgctggcca gccaactgcc ggtcgtcgtg
     2761 gtgctgccga cgacgccggt tggccccggc gaccgccgac cgaccccgac cggcaagctg
     2821 atcgtcgact tcatgcgcgg ccgcatgttc ggctacgtgc cgggggggat gaatctggtg
     2881 ccggtggagg atgtggcccg ggcgcacgtg ctggcgctcg cgcgcggccg cccgggcgag
     2941 cgctacctgc tcggcggcga caacctcacc ttcgccgaga tctgggccac gctggctgag
     3001 ctctgcggcc gccccgcgcc gcgcctgcgc gtgccctacg ggctggcgct caccctcggc
     3061 tgggccgacg agctgcgctg ccgcgcgcgg ccgggccagg agccgctggt gccgctcgaa
     3121 ggcgtcgcga tgtcgcggca ctacatgttc gccagcagcg acaaggcggg ggcggagctg
     3181 gggttcgcgc cgggctcgag ccgcgcggcg ctggcccgtg ccgtcgcgtg gttccgcgag
     3241 cagcctgatg cctgagagcg gcagcccgcg cgtgctggcg cccaccgtcg tcgagtacct
     3301 ggcggcccgg ctcgtcctgg gccggcacgc gccgcgctat gccggcgtcg gcctgcggcg
     3361 ctggcagggt acgcctggcg acgcgccggt cgtcgtctgc ggcctggccg gggcgttgac
     3421 ggatgatctg ccctcgggct gcgtgctgat ccccgagaac gtcgcgctgc cgggcggccc
     3481 gccgcgtgac tgcgaccccg agctggtcgc ggccttcacg acggccgcgc gttcgcttgg
     3541 ctatgaggtg cgcagcggac cgctggtcac cgtgcccgtt ctggccagca gcgaggcgcg
     3601 cgcacggctg acggcgagcg gcttcgcggc ggccgacatg gaaaccgcgc tgctgcccga
     3661 caagctgcgc atcgccaccg tgcgcgtgat cctcgacacg ccccagcgcc cgctctccgc
     3721 ccgctggctc aagcccaccg ccgccctgcg ccccgcgctc tggcccgagc ttctctggct
     3781 cgcacgccac gcccccgcct acgcgtaccg ggctgggcgt gtggtccgcg caggtctagg
     3841 cgccgtctga cggacgcctt ggccgggaga gcacggatcc ttcgactgcg cccaggatga
     3901 cagggtagag caggggcggg cgaaggcgac gtgacgtact cgtacgct
//
LOCUS       DASUBI010000738         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1027541, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000738 DASUBI010000000
VERSION     DASUBI010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027541"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /gene="trpB"
                     /locus_tag="VFU72_14405"
     CDS             <1..800
                     /gene="trpB"
                     /locus_tag="VFU72_14405"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5424733.1"
                     /translation="CVVYMGELDIARQSLNVFRMKLLGAEVRPVASGSRTLKDAMNEA
                     IRDWVTNVGDTYYLIGSAAGMHPYPMMVRDFQSVIGRETRRQMLEQVGKLPNAVVACV
                     GGGSNAIGIFHPFIEDSGVELHGAEAAGLGIASGKHAASLSAGRVGVLHGSRSYVLQD
                     EDGQIIEAHSISAGLDYPGVGPEHSYLKDTGRASYHPVTDAEALEGFRQLCLSEGIIP
                     ALESAHAVYLGVQLAAGRSPDETLVINLSGRGDKDMHTVAEALGVEL"
     gene            797..1600
                     /gene="trpA"
                     /locus_tag="VFU72_14410"
     CDS             797..1600
                     /gene="trpA"
                     /locus_tag="VFU72_14410"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5424734.1"
                     /translation="MTVTTGRSRIAETFARLKERGERALMPYLTVGYPELDTVQRVVP
                     ALVAGGATMVELGIPFSDPLADGATVQHSSHQALLNGVTVDYCLESAAGLRAGGVDVP
                     LIFMGYYNPMLNYGLERFAARCAEAGVDGLIVPDLPPGESHALQAACRQYGRDLIFML
                     APTSTEALIDEVARQASGFIYCVSLTGVTGARERLAEGVGEFLARVRARTDLPLALGF
                     GISRREHVAQASELADGVVVGSALVSRLGETPRAEQAAVVEAYMRELRG"
     gene            1644..2057
                     /gene="aroH"
                     /locus_tag="VFU72_14415"
     CDS             1644..2057
                     /gene="aroH"
                     /locus_tag="VFU72_14415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU5424735.1"
                     /translation="MMRCRGIRGATTVERNDAEEMLGATRELLAALIEANQIEVEDVA
                     SVIFTTTPDLTAVFPAVAARDEGWDTVALICGHEMAVPGALDHAIRVLIHWNTEKTAS
                     EIRHVYLRRAVELRPEWAYRPGQALQGLGGNGSRP"
     gene            2054..>2377
                     /locus_tag="VFU72_14420"
     CDS             2054..>2377
                     /locus_tag="VFU72_14420"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037053.4"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5424736.1"
                     /translation="MIIVVEPHTSEQQVAEIVGRVERAGFRTRQIIGQDQSIIGVVGT
                     PIPDDLYETIGLLPGVQQVMRVSKRYKLVSRDFHPLDTVVPVRDVLVGGPEVVTIAGP
                     CSVESE"
BASE COUNT          368 a          764 c          892 g          353 t
ORIGIN      
        1 agtgcgtggt ctacatgggt gagctggaca tcgcgcgcca gtcgctcaat gtctttcgca
       61 tgaagctgct gggtgccgag gtgcggccgg tcgcctccgg cagccgcacg ctgaaggacg
      121 ccatgaacga ggccatccgc gactgggtca ccaacgtggg cgacacctac tacctgatcg
      181 gctcggcggc cgggatgcac ccctacccca tgatggtgcg cgacttccag tcggtgatcg
      241 ggcgtgagac gcggcgccag atgctggagc aggtcgggaa gctccccaac gcggtcgtcg
      301 cctgcgtcgg cggcggctcg aacgccatcg gcatcttcca ccccttcatc gaggacagtg
      361 gggtcgagct gcacggcgcg gaggcggccg ggctggggat cgccagcggc aagcacgccg
      421 cctcgctcag cgccgggcgc gtcggggtgt tgcacggctc gcgctcgtat gtcttgcagg
      481 acgaagacgg ccagatcatc gaggcgcata gcatctcggc cgggctcgac taccccggcg
      541 tcgggccgga gcacagctac ctgaaggaca cgggccgcgc cagctaccat ccggtgacgg
      601 atgccgaggc gctggagggc tttcgacaac tctgcctgag cgaggggatc atcccggcgc
      661 tggagtcggc ccacgcggtc tacctgggcg tccagctcgc cgccgggcgc tcgccggacg
      721 agacgctggt gatcaacctc tcgggccggg gcgacaagga catgcacacg gtagcagagg
      781 cgctgggggt ggagctatga ccgtgacgac cggacgcagc cgcatcgcgg agaccttcgc
      841 gcggctcaag gagcgcggtg agcgcgccct gatgccctac ctgaccgtcg gctacccgga
      901 gctggacacg gtgcagcggg tggtgccggc gctggtagcg ggcggcgcga cgatggtgga
      961 gctgggcatc cccttttccg atccgctggc cgacggcgcc accgtccagc actccagcca
     1021 ccaggcgctc ctgaacggcg tgacggtcga ctactgcctg gagagcgcgg cggggctgcg
     1081 cgcgggcggc gttgacgtgc ctctgatctt catgggatac tacaatccaa tgctcaacta
     1141 cggtctcgaa cgcttcgcgg cgcgctgcgc cgaggccggt gtcgatgggc taatcgtgcc
     1201 cgatctgccg cctggggaga gtcacgcgct ccaggcggcc tgtcgtcagt acgggcgcga
     1261 tctgatcttc atgctggcgc cgaccagcac cgaggcgctg atcgacgagg tggcgcgtca
     1321 ggcgagcggg ttcatctact gcgtctcgct gaccggcgtg accggggcgc gcgagcgcct
     1381 ggcagagggc gtgggcgaat tcctggccag ggtgcgtgcc aggaccgacc tgccgctggc
     1441 gctcggcttc ggtatctcgc gccgcgagca cgtggcgcaa gccagcgagc tggcggacgg
     1501 cgtggtggtc ggtagcgcgc tggtcagccg gctgggcgag acaccgcggg ccgagcaggc
     1561 ggcggtggtg gaggcgtaca tgcgcgagtt gcgcggctga gcctcccgcc ccggctggcc
     1621 ggttacaggg agggaatcgg cggatgatgc gctgccgggg cattcgcggc gcgacgacgg
     1681 tggagcggaa cgatgcggag gagatgctcg gcgccacacg agaactcctg gcggcgctga
     1741 tcgaggccaa ccagatcgag gtcgaggacg tcgccagcgt gatcttcacc acgacaccgg
     1801 acctcaccgc cgtcttcccg gcggtggcgg cgcgcgacga gggctgggac accgtggcgc
     1861 tgatctgcgg ccacgagatg gcggtgcccg gcgcgctcga ccacgcgatc cgcgtcttga
     1921 tccattggaa caccgagaaa acggccagtg aaatccgtca cgtctatctc aggcgggcgg
     1981 tcgagttgcg gccggagtgg gcctatcggc ccgggcaggc gctacagggg ctgggaggga
     2041 acgggagcag gccatgatca tcgtcgtcga gccgcacacg agcgagcagc aggtcgcgga
     2101 gatcgtcggg cgcgtggagc gggccggctt ccgcacgcgc cagatcatcg ggcaggatca
     2161 gtcgatcatc ggcgtggtcg gcacgccgat ccccgacgat ctctacgaga cgatcgggct
     2221 gctgcccggc gtgcagcagg tgatgcgcgt cagcaagcgc tacaagctgg tcagccgcga
     2281 cttccacccg ctcgatacgg tcgtgcccgt gcgcgacgtg ctggtcggcg ggccggaggt
     2341 ggtcacgatc gccgggccgt gctcggtcga gagcgag
//
LOCUS       DASUBI010000739         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_700818, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000739 DASUBI010000000
VERSION     DASUBI010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700818"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFU72_14425"
     CDS             <1..1060
                     /locus_tag="VFU72_14425"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5424737.1"
                     /translation="NNPATVTATNPRLTDAQRKNIQLYTVAPTRFRRTVETTAAVDFD
                     NDQATSVLAPFSGPVSRTLVSIGDEVTQGQALARVDSPDFATAISTYQKAIATARTNR
                     RLADLDKDLFQHNGVSQREYEQAQTDATNAEADRDAALRALIALNVPTDTLDAIREGR
                     PVSTPAGIIRSPVAGTVVEKLVTPGQLLEAGTTPCFTVANLSRVWVMAQLFGTDLAAV
                     SVGDPAEVVTGVDAKNFPGKLDKISALVDPNTRAVAVRVVVDNPQHSLRKQMYVRVLI
                     HSRQESTGLLIPISAVLRDEENLPFVYVAQADGSFARRPITLGSRVADRYEVPTGLRA
                     GDQVVVSGGLFVQFMQSQ"
     gene            1057..>2201
                     /locus_tag="VFU72_14430"
     CDS             1057..>2201
                     /locus_tag="VFU72_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5424738.1"
                     /translation="MSATPPHGAPAPRSGSIINRIVASSLRQRFLVVLMTIVLIGAGA
                     RSLNRLPVDAYPDLSPPMVEVITQWPGHAAEEVERLITVPTEREMNGLPRLTTSRSIS
                     LYGLSDVILTFQNGTDDYFARQQVFNRVGDVSLPSGVTPSLSPLSPPSGLIYRYVLES
                     PDRSPMELKTFEDWTIEPQYRSVPGVADDSGFGGGSMQYQVLLDPARVASVGLSVPQV
                     EAALTANNGNAGGGFYSQGGQFYYVRGLGRLKTLEDIGNVVVAVHDGTPVLVKDVGRV
                     AIGIAPRLGEFGYMDQDDAVEGVILLRTGEKTQDVLKRVEAKTKELNTEILPKDVKVV
                     PFYDRSDLIALTTRVVEDNLLRGMALVIIVLIFFLYDFRAGLIVALT"
BASE COUNT          382 a          728 c          712 g          379 t
ORIGIN      
        1 caacaacccg gcgacggtga ccgctaccaa tccgcgactc acggacgcgc agcgcaagaa
       61 catacagctc tacacggtcg ctccaacacg ctttcgccgt acggtggaga cgacagcggc
      121 ggtcgatttc gacaacgacc aggccaccag cgtgctggcg cccttctccg ggccggtatc
      181 gcgcactctg gtgtccatcg gtgacgaggt cacgcaggga caggcgctcg cgagggtcga
      241 ttcaccggac ttcgcaacgg ccatcagtac gtatcagaag gccatcgcta cggcgcgcac
      301 caaccggcgg ctcgcggacc tggacaaaga cctgtttcag cacaacgggg tctcgcagcg
      361 cgaatacgag caggcgcaga cggacgccac caacgccgaa gccgatcgcg acgccgcgct
      421 ccgggcgctc atcgcgctga acgttccgac tgacaccctc gatgcgatcc gcgaaggacg
      481 tcccgtgtcg acgccggctg gcatcattcg ctcgcccgtg gccggaacgg tggtggagaa
      541 gctcgtcacg cccggacagc tgctggaagc cgggaccacg ccttgcttta ccgtggccaa
      601 cctgtcgcgc gtgtgggtca tggcacaact gttcggcacg gacctcgccg ccgtgagtgt
      661 cggggatccg gcagaagtcg tgaccggggt cgatgcgaag aactttccgg gcaaattgga
      721 caagatctcg gccctggtgg accccaacac gcgcgccgtg gccgtgcggg tggtcgtgga
      781 caatccgcag cactcgctgc gcaagcagat gtacgtgcgc gtgctgattc attcccgcca
      841 ggagagcacc gggctcctga ttccgatctc ggcggtgttg cgcgacgagg agaacctgcc
      901 gttcgtctac gtcgcccagg ctgacggcag cttcgcgcgc cggccgatca ctctgggctc
      961 gcgagtggcg gatcgatatg aggtgcccac cgggttgcgc gccggcgacc aggttgtggt
     1021 gtcggggggc ctctttgtgc agttcatgca aagccaatga gtgcgacgcc tccgcacggg
     1081 gcacctgcgc cgcgcagcgg ctcgatcatc aaccggatcg tcgcttcctc gctgcgccag
     1141 cgcttcctcg tggtgctgat gacgatcgtg ctgatcggcg ccggcgcacg ctccctgaac
     1201 cgcctgccgg tggacgcgta tccggatctg tctccgccga tggtggaagt gatcacgcag
     1261 tggccgggac acgccgcgga agaagtcgaa cggctgatca ccgtccccac cgaacgcgag
     1321 atgaatggcc tgccccggct gaccaccagc cgttcgatct ccctctacgg gctgtcggac
     1381 gtcatcctga ccttccagaa cgggacggat gactatttcg cgcggcagca ggtgttcaac
     1441 cgggtgggcg acgtgtccct gccgagcggg gtcacgccca gcctgtcgcc gctgtccccg
     1501 ccttccgggc tgatctatcg ctacgtgctg gagagccccg atcgctcgcc gatggagctg
     1561 aagacattcg aggactggac gatcgagccg caataccgct ccgtgccggg cgtcgccgat
     1621 gactccggct tcggcggcgg cagcatgcag taccaggtgc tgctggatcc agcgcgcgtc
     1681 gcctccgtcg gcctgtcggt gccgcaggtc gaggcggcac tgaccgccaa caacggcaat
     1741 gccggcggcg gcttctactc gcagggcggc cagttctact acgtgcgcgg cctgggtcgc
     1801 ctcaagaccc tcgaggacat cggcaacgtc gtggtggcgg tgcacgacgg caccccggtc
     1861 ctggtcaagg acgtcggtcg cgtcgcgatc ggcatcgccc cgcgcctggg cgagttcggc
     1921 tacatggatc aggatgacgc cgtggaggga gtcatcctgc tgcgcaccgg cgagaagacg
     1981 caggacgtgc ttaagcgtgt cgaagccaag acgaaagaac tcaacactga aatcctgccc
     2041 aaggacgtga aggtcgttcc gttctatgac cgcagcgacc tgatcgcgct gaccaccagg
     2101 gtggtggaag acaatcttct gcgcggcatg gcgctcgtca tcattgtgct gatcttcttt
     2161 ctttatgatt ttcgcgccgg cctcatcgtc gcactcacca t
//
LOCUS       DASUBI010000740         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_88045, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000740 DASUBI010000000
VERSION     DASUBI010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88045"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFU72_14435"
     CDS             complement(<1..389)
                     /locus_tag="VFU72_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5424739.1"
                     /translation="MATRTNPRSAGLHVADSHDLIRVIGARENNLKDVSIEIPKRRLT
                     VFTGVSGSGKSSLVFGTIAAESQRMVNETYSSFVQGFMPSLARPDVDVLEGLTTAIIV
                     DQERMGANPRSTVGTATDAHAMLRILF"
     gene            complement(491..979)
                     /locus_tag="VFU72_14440"
     CDS             complement(491..979)
                     /locus_tag="VFU72_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5424740.1"
                     /translation="MAERKPAKSGTQQSTKQASGQASTGFTGEERAAMAERVQEQRAA
                     ARRGSSSKADGENDVLAKIAEMPESDRVMAERLHALIQEAAPELSPRTWYGMPAYAKG
                     GQVLCFFQSAQKFKSRYATLGFSDAANLDDGAMWPTAYALQELTAADEARIKALVKQA
                     VS"
     gene            complement(1029..1451)
                     /locus_tag="VFU72_14445"
     CDS             complement(1029..1451)
                     /locus_tag="VFU72_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5424741.1"
                     /translation="MSETVVLDVEVKSPIERVWHALTDSATLSRWMLFKSNDFKPVIG
                     HAFQFSGAPGWDGVVECEVIEVEEPHRLSYSWASAGEGDTPHATVVTWTLTEAGAGIT
                     RLHLEQGGFRPEAKQEIGGARWGWTTMLDQLQGVLAPA"
     gene            complement(1448..1762)
                     /locus_tag="VFU72_14450"
     CDS             complement(1448..1762)
                     /locus_tag="VFU72_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5424742.1"
                     /translation="MRDVYSALADPTRRRLLRLLAEDDELSLHELTPEFPMGRTAISK
                     HLAVLKEAGLVTDHKVGRETRYHLNAAPLREIQEWLSFYERFWSKRIDHLKDLLESEE
                     YA"
     gene            complement(1957..3807)
                     /locus_tag="VFU72_14455"
     CDS             complement(1957..3807)
                     /locus_tag="VFU72_14455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5424743.1"
                     /translation="MEATDPDLFSSGELAASALDRQIDLSAISWYALGWAAVLLLAAA
                     IRFAGLRAWPLAAGEAGIASDAYQLLHGGAISAAGAAHPLATALASLSFFLFAAGDGA
                     ARLVPLLAGLATIALCWPLRDTLSRPAVLGAALVAALSPALTFAGQRLDGAALLTLSS
                     LLLLLALRRAGRRPGFGNALLAGVAAALLPLSDPLGWLALPLVAGFALVLLPRDERPA
                     ATMLPGVVLGAASAVLLVTSSLLIHPAGLSDFFSASLGQLWHQQLGNLGQLWQLPVFE
                     LVIDDLPALVFAIVGLAVFRRKSHAARVLAWWSAAALVLASLLGAKDTASYALVALPL
                     ALLAGYGLAWLIARVRWPVFGTYSGLGFAVALIATIAAAASALGVILGMPSGGSLHEV
                     VKFVLLVVLILAPLLLVTIRLGRALNGTSWPLLALVGVALLLGLGLRTSTLLASTTES
                     RGGDILTANSTSPDVGSLVERLRTISRDMTDFKQSALDPAGGHGLTIAADDALAEPFR
                     WYFRDFPSFQVAPDASAVTPAPQVVISSAPPLEHTSRAARSFAYRTTLAASLARPNWG
                     HLVFAVADPNQIRGFLGFLIDHKVANPPPAQQFTLSLSPDVGARLFGVTP"
     gene            complement(3822..>4903)
                     /locus_tag="VFU72_14460"
     CDS             complement(3822..>4903)
                     /locus_tag="VFU72_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HEU5424744.1"
                     /translation="RGLLICWALGLLAVLSWAGEKMPWLTVHIALPLTLLAASYIGDA
                     LTWFERRRQARGAVVLGGLALGLLGAAFLVLAWASDGQYVTTPAGSARGVRADVLSHW
                     WQTVYLPWFLLLAAVAIGIWRLGARRALLALLLAATLGLSLAEVHAGWRMTYRQGDVP
                     LDMLIYVQTSPDVPRVTHDLAELSQQQTGGMGLEVWYDDIVQWPFNWYLHGFTNRRFF
                     GNKLPTDQPINAPVIIISDDYVTPAVEAALHDYTYQEYPLRWWFPEEDTYRRFAYAPD
                     LKNEGRQNYQDSAKPPFTLTQTALSALRSIWSLHRPQEQAKMFRLVAYRELPSQLGDY
                     NFRVYVRNDLMPSFNSIRYGSGTDN"
BASE COUNT          799 a         1642 c         1744 g          718 t
ORIGIN      
        1 ctgaagagga tgcgcagcat cgcgtgggcg tcggtggcgg tgccgacggt ggagcgcggg
       61 ttggcgccca tccgctcctg gtcgacgatg atcgccgtcg tcagcccctc gagcacgtcg
      121 acatcggggc gcgccaggct cggcatgaac ccctggacga agctgctgta ggtctcgttg
      181 accatccgct ggctctccgc ggcgatcgtc ccgaagacca gcgagctctt gccggacccg
      241 gacaccccgg tgaagaccgt cagccggcgc ttcgggatct cgatgctgac gtccttgaga
      301 ttgttctcgc gcgcgccgat cacacgaatc agatcgtggc tgtcggcaac atgcagcccg
      361 gccgaccgcg ggttcgtcct cgtggccatg ctcaccgttt ctccagctgt caggcggaag
      421 cgtcgcgcgg acgacacctc gtagcgatct gcgacagcgt ccagcgcgga ccgattggcc
      481 ggctcagcct tcagctcacg gcctgcttca cgagggcttt gatccgtgcc tcgtcagcgg
      541 cggtcagctc ctgcaacgca taggcggtcg gccacatggc gccgtcgtcg aggttcgccg
      601 cgtcgctgaa gccaagcgtg gcgtacctcg acttgaattt ctgcgcgctt tggaagaagc
      661 agagaacctg gccacccttg gcgtaggccg gcataccgta ccaggttcgt ggtgagagct
      721 ccggcgccgc ctcctggatg agcgcgtgga gccgctcggc catgacgcga tccgactccg
      781 gcatctcggc gatcttggcg agcacgtcgt tctcaccgtc tgccttcgaa ctcgaaccgc
      841 ggcgcgcggc cgcccgctgc tcttgcacgc gctccgccat agcggcccgc tcttcgccag
      901 taaaccccgt ggaggcctgc ccgctggctt gcttggtcga ttgctgggtg cccgacttgg
      961 ccggtttccg ttccgccatc gtgtcctcct tcggtgccgt ccgtgccgct cgtggctgtg
     1021 atcgagggct acgccggagc caaaacgccc tgcagttgat ccagcatggt cgtccaaccc
     1081 cagcgggcgc cgccgatctc ctgcttggcc tcgggtcgga aaccgccctg ctcgagatgc
     1141 aaccgcgtga tgccggcacc ggcctcggtc agggtccagg tgaccaccgt cgcgtgcggt
     1201 gtgtcgccct ccccagcgct tgcccagctg taggagagcc ggtgcggctc ctccacctcg
     1261 atcacctcgc actcgaccac gccgtcccag cccggcgccc cgctgaattg aaacgcgtgc
     1321 ccgatgaccg gcttgaaatc gttcgacttg aacagcatcc acctggagag cgtggcggag
     1381 tcggtcaggg cgtgccagac ccgctcgatc gggctcttga cctcaacgtc cagcacgacg
     1441 gtctcactca tgcgtactcc tccgactcca acaggtcttt caaatgatcg atccgcttgg
     1501 accagaagcg ctcgtagaac gagagccact cttgaatctc gcgcaaggga gcggcgttca
     1561 agtgatagcg cgtctcccgc ccgaccttgt gatcggtcac gagtccggcc tccttgagta
     1621 ccgccagatg cttcgagatg gccgtgcggc ccatcggaaa ctcgggcgtc agctcatgga
     1681 gggacagctc atcgtcctcg gccagcaggc ggagcagccg gcggcgcgtc ggatcggcca
     1741 atgcgctgta gacatctcgc atggggctga cgtcctctgc ctgtgtcgca ccgctggggc
     1801 cggcgcgcgc cggcccgcta agcgacacca tttggtgtcg ggatccataa tacgactcca
     1861 aatggtgtcc tgtcaagtcc ggtgttggcg agtgagccgc gccggccaac gagcaccccg
     1921 ctcccgaacg atcgcggctc cgtgcccggg ccacgcctac ggcgtgacgc cgaagaggcg
     1981 cgcgccgaca tcggggctga gcgagagcgt gaactgctgc gccggcggcg ggttggcgac
     2041 cttgtgatcg atcaggaacc cgaggaaccc gcgaatctga ttggggtcgg ccaccgcgaa
     2101 gacgaggtgt ccccaattcg ggcgcgccag cgaggccgcc agtgtcgtgc ggtaggcgaa
     2161 ggagcgcgcg gctcggctgg tgtgctcgag cgggggcgca ctggagatca ccacctgcgg
     2221 cgcgggcgtg acggcgctgg catccggcgc cacctggaag ctggggaagt cgcggaagta
     2281 ccagcggaac ggctcggcca gcgcgtcgtc agcggcgatg gtcaggccat ggccaccggc
     2341 cggatcgagc gcgctctgct tgaagtcggt catgtcgcgg ctgatcgtgc gcaggcgctc
     2401 caccagcgag ccgacatcgg ggctggtgct gttggcggtc aggatgtcgc cgccgcgcga
     2461 ctcggtggtc gacgccagca gggtgctggt gcgcaggccg agccccagga gcagcgccac
     2521 accgactagc gccagcagcg gccaggatgt gccgttgagc gcccgtccca gccggatggt
     2581 cacgagcagc agcggcgcca ggatcagtac gacgagcagc acgaacttga ccacttcgtg
     2641 caggctgcca ccgctgggca tgccgaggat gacgccgagc gcgctggcgg ccgccgcgat
     2701 ggtggcgatc agcgcgacgg cgaagccgag ccccgagtag gtgccgaaga ccggccagcg
     2761 cactcgggcg atgagccagg ccaggccgta gccggccagc agcgccagcg gcagcgcgac
     2821 cagcgcgtag ctggcggtgt ccttggcgcc cagcagcgag gccagcacga gggcagcggc
     2881 gctccaccag gccagcacgc gcgccgcgtg agacttgcgg cggaaaacgg ccagccccac
     2941 tatcgcgaac accagcgccg gcaggtcgtc gatcacgagc tcaaagaccg gcaactgcca
     3001 gagctggccg agattgccca attgctgatg ccagagctgg ccgaggctgg cgctgaagaa
     3061 gtcgctgagg ccggcggggt ggatcagcag gctgctggtc accagcagca cggcgctggc
     3121 ggcgccgagg accacgccgg gcagcatggt cgccgccggg cgctcgtcgc gcggcagcag
     3181 caccagtgcg aagccggcga ccagcggcag ggcgagccag ccgagcggat cgctcagcgg
     3241 caggagcgcc gccgccaccc cggccagcag cgcgttgccg aagccgggcc ggcgtcccgc
     3301 ccggcgcagc gccagcagca acaggaggga cgagagtgtc aagagcgccg cgccgtcgag
     3361 ccgctggccg gcgaaggtca gcgccggaga gagcgcggcc accagcgcgg cgccgagcac
     3421 ggctgggcgg ctgagggtgt cgcgcagggg ccagcagagt gcgatggtgg cgaggccggc
     3481 cagcagcggg accagacgcg cggcgccatc acccgcggcg aagaggaaga agctgagcga
     3541 ggccagcgcg gtcgccagcg ggtgcgctgc ccctgccgcc gagatcgcgc ctccgtgcag
     3601 cagctggtag gcgtcgctgg cgatgccggc ctcgccggcc gcgagtggcc aggcgcgcag
     3661 gccggcgaag cggatcgccg ccgccagcag caggacggcg gcccagccga gcgcgtacca
     3721 ggagatcgcg ctcaggtcga tctggcggtc aagcgcgctg gcggctaatt cgccgctact
     3781 gaagagatcg ggatcggtag cctccaaagc gggcgtctcc tctagttgtc cgtgccgctg
     3841 ccatagcgaa tgctgttgaa gctcggcatg agatcgttgc gcacgtaaac gcggaagttg
     3901 tagtcgccga gttgcgaagg aagctcgcga taggcgacca gtcggaacat cttggcctgc
     3961 tcctggggcc ggtgcagcga ccagatgctg cgcagcgcgc tcagagcagt ttgggtcagc
     4021 gtgaagggcg gcttggcgga atcctggtag ttctgccggc cctcgttctt gagatcgggg
     4081 gcgtaggcga agcggcggta ggtgtcctcc tcggggaacc accagcgcag cgggtactcc
     4141 tggtaggtgt agtcgtgtag cgccgcctcg accgccgggg tgacgtagtc gtcggagatg
     4201 atgatcaccg gcgcgttgat gggctggtcg gtgggtagct tattgccgaa gaagcgccgg
     4261 ttggtgaagc cgtgcaggta ccagttgaag ggccactgga cgatgtcgtc gtaccagact
     4321 tcgagcccca tgccgccggt ctgctgctgc gagagctcgg ccaggtcgtg cgtcacgcgc
     4381 ggcacgtccg gcgaggtttg cacgtagatc agcatatcga gcggcacgtc gccctggcgg
     4441 taggtcatgc gccagccggc atgcacctcg gccagcgata gcccgagcgt cgcggccagg
     4501 agcagcgcca gcagcgcgcg gcgcgcgcca aggcgccaga tgccgatcgc gacggccgcc
     4561 agcagcagga accaggggag atagaccgtt tgccaccagt gcgacagcac gtccgcgcgc
     4621 acgccgcgcg cggagccggc cggggttgtg acgtactgac catcgctggc ccaggccagc
     4681 accaggaagg cggcgccgag taggccgagc gccaggccgc cgagcacgac cgcaccgcgc
     4741 gcctggcggc gccgctcgaa ccaggtcagc gcgtcgccga tgtagctggc ggccagcagc
     4801 gtcagcggca gggcgatgtg cacggtcagc cagggcatct tctcgccggc ccaggagagc
     4861 acggccagca ggccgagcgc ccagcagatg agcaggccgc gca
//
LOCUS       DASUBI010000741         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_692485, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000741 DASUBI010000000
VERSION     DASUBI010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692485"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..670
                     /locus_tag="VFU72_14465"
     CDS             89..670
                     /locus_tag="VFU72_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424745.1"
                     /translation="MQPIGRRIIVVGSSNAGKSTLAEQLAARLGVPFIELDALHWEPG
                     WIEAGREVFHERVRRAIAPDSWVMAGNYLSQQQDVSWPAADTIIWLDLPLATVLRRCV
                     ARTWRRWRTREQLWGTNQENVWEHLMLWNPDKSLIAYTVKTHQRRRHEFAAMTRDPRW
                     SHLRFVRLRSPEEVARWLAEVVGAPVASMRRET"
     gene            complement(823..1329)
                     /locus_tag="VFU72_14470"
     CDS             complement(823..1329)
                     /locus_tag="VFU72_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5424746.1"
                     /translation="MIETERLLLRPMVAEDAVPLLRVFADPLVMASFGVPPFGPEQME
                     RWVARNLAHQSTQGYGLFSVVLKESGLLIGDCGLTRMTLGGVDEVELGYDLCSDYWNR
                     GLATEAACAVRDDAFATLGLPRLVSLIRQGNAASRRVAEKVGMRHEADLVEDGRDYWL
                     YSLERGSG"
     gene            complement(1339..2343)
                     /gene="hpaD"
                     /locus_tag="VFU72_14475"
     CDS             complement(1339..2343)
                     /gene="hpaD"
                     /locus_tag="VFU72_14475"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HEU5424747.1"
                     /translation="MSAPQRPFAILKAGHAEIRVTDLDRARDFYVELLGFVESERAGD
                     RLYLRCLEDWEHHSLVLRQAPSPGLGHIAYRVARDADLDLLEQLAAARGLPRRWVAPG
                     EERGQGRALRIQDPLGFPVEFYHDVERVERLLQSFHRYHGPHVMRVDHFNLQVPDVQR
                     ASDWYMGELGFACAEYTDSDDQPPRLWATWLHRKQNVHDVALMTGPGPRLHHLGFWTQ
                     EPGNVLRAADILAGAGHEAAIERGPGRHGISNAMFLYLRDPDENRIELYTSDYLIPDP
                     DFEPIRWSLSDPRRQTFWGTAAPPSWFNDAALVERIGGSGFVPVEAPLLADRPSFVGH
                     "
     gene            complement(2340..2948)
                     /locus_tag="VFU72_14480"
     CDS             complement(2340..2948)
                     /locus_tag="VFU72_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5424748.1"
                     /translation="MGFREVEPDQMSFRRTLGLFATGVGLITVQAGTRIHGMTANAIS
                     SVSLEPPLVLACVGKQARLAGLVRETGGFTINFLSRDQEAVARHFAGARASALPPEVH
                     FESWECAPRLQGALSAVGCHLVQAVDAGDHLILIGRVVAIAEGAHDAQPLLFYHGGYR
                     RLAPAGEHVTAEPDLMTSTGASLYYDEWSEHPSGHSHGRHEP"
BASE COUNT          451 a         1059 c         1084 g          453 t
ORIGIN      
        1 cgcgttgccg cagggggagg tcgtggtgct tctcaccggc tcgggcctga aggccaccga
       61 cacgatccgc gccgtcctgg gctgagccgt gcaacccatc ggcagacgca tcatcgtcgt
      121 cggctcctcc aacgccggca agagcacgct ggcggagcag ctcgccgcgc gcctgggcgt
      181 gcccttcatc gagctggatg ccctccactg ggagccaggc tggatcgagg ccgggcgcga
      241 ggtcttccac gagcgcgtgc ggcgggcgat cgcgcccgac tcctgggtca tggccggcaa
      301 ctacctctcc cagcagcagg acgtcagctg gcccgccgcc gacaccatca tctggctcga
      361 tctgccgctg gccaccgtgc tgcgccgctg cgtggcgcgc acctggcgcc gctggcgcac
      421 gcgcgagcag ctttggggca ccaatcagga gaacgtctgg gagcacttga tgctctggaa
      481 cccggacaag tcgctgatcg cctacaccgt gaagacgcac cagcgccggc ggcatgaatt
      541 cgcggccatg acgcgcgacc cgcgctggtc gcacctgcgc ttcgtgcggc tgcgctcgcc
      601 ggaggaggtg gcacgctggc tggcggaggt tgtgggggcg cccgtcgcct caatgcggcg
      661 cgagacgtag ggcgcgacgg atccttcgac tgtgcgtcag gatgacgggg tgaaatttga
      721 ggcggacgag gaagactcgc ctcgtccggt aggccccatc ccctaccctg tcatcctgag
      781 ctcgtcgaag gatccgtgct gacccggcac caaagtctgt cgtcagccac taccccgctc
      841 cagggaatac aaccagtagt cgcgaccgtc ctcgacgagg tcggcctcgt ggcgcatccc
      901 gaccttctcg gccactcggc gcgaggctgc gttcccctgg cggatcaggc tgaccagccg
      961 cggcaggccg agcgtggcga aggcgtcgtc gcgcaccgca caggcggcct cggtcgccag
     1021 gccccggttc caatagtcgc tgcagagatc gtagccgagc tccacctcgt ccacgccgcc
     1081 gagcgtcatg cgcgtcagcc cgcagtcgcc gatcagcagg ccgctctcct tcagcacgac
     1141 cgagaagagc ccgtagccct gtgtcgactg gtgcgccagg ttgcgggcca cccagcgctc
     1201 catctgctcg gggccgaagg gcggcacgcc gaaggaggcc atcaccagcg ggtcggcgaa
     1261 gacgcgcagc agcggcacgg cgtcctccgc caccatcggg cgcagcagca ggcgctcggt
     1321 ttcgatcacg acgcgtcgtc agtgccccac gaagctgggg cggtcggcca gcagcggcgc
     1381 ctcgaccggg acgaagccgc tgccgccgat gcgttccacc agcgcggcgt cgttgaacca
     1441 gctcggcggc gcggccgtcc cccagaaggt ctggcgccgg ggatcgctga gactccagcg
     1501 gatcggctcg aagtcggggt cggggatcag gtagtcgctg gtatagagct cgatgcggtt
     1561 ctcgtcgggg tcgcgcaggt agagaaacat ggcgttggag ataccgtgcc ggccggggcc
     1621 gcgctcgatg gccgcttcat gcccggcgcc ggccaggatg tcggccgcgc gcaagacatt
     1681 gcccggctcc tgggtccaga agccgaggtg gtgcagccgc gggccggggc cggtcatcag
     1741 cgccacgtcg tgcacgttct gcttgcggtg cagccaggtg gcccacaggc gcggcggctg
     1801 gtcgtcgctg tcggtgtact cggcgcaggc gaagcccagc tcgcccatgt accagtcgct
     1861 ggcgcgctgc acgtccggca cctgcaggtt gaagtggtcg acgcgcatga cgtgcgggcc
     1921 gtggtagcga tggaagctct gcagcaggcg ctcgacgcgc tcgacgtcgt ggtagaactc
     1981 gaccgggaag ccgagaggat cctgaatgcg cagcgcgcgg ccctggccgc gctcctcgcc
     2041 gggcgcgacc cagcggcgcg gcagcccccg cgctgcggcc aactgctcca gcagatcgag
     2101 gtcggcgtcg cgcgccaccc gataggcgat gtggcccagg ccggggctcg gcgcctgacg
     2161 cagcacgagg ctgtggtgct cccagtcctc caggcagcgc aggtagagcc ggtcgccggc
     2221 gcgctcgctc tcgacgaagc cgagcaactc gacgtagaag tcgcgtgccc ggtcgaggtc
     2281 ggtgacgcgg atctcggcgt ggccggcttt gagaatggcg aagggtcgtt gtggggcgct
     2341 catggctcgt gcctcccgtg gctgtgcccc gaagggtgct cgctccactc gtcgtagtac
     2401 aggctggcgc cggtgctggt catcaggtcg ggctcggccg tgacgtgctc gccggcgggc
     2461 gcgagccgcc ggtagccgcc gtggtagaag aggagcggct gggcgtcgtg cgcgccctcg
     2521 gcgatcgcaa cgacgcggcc gatcaagatg aggtgatcgc cggcgtcgac cgcctgtacc
     2581 aggtggcagc ccaccgccga cagcgccccc tgcaggcgcg gcgcgcactc ccaggattcg
     2641 aaatgaacct cgggcgggag cgcgctggcg cgcgcgccgg cgaagtggcg tgcgacggct
     2701 tcctggtcgc ggctgaggaa attgatcgtg aagccgccgg tctcgcgcac cagcccggcc
     2761 agccgcgcct gcttgccgac gcaggccagt accagtgggg gctccagcga aacggaggag
     2821 atggcgttgg cggtcatgcc gtggatgcgc gtgcccgcct gcacggtgat cagccccacg
     2881 ccggtggcga acaggcccag cgtacggcgg aagctcatct gatccggctc aacctcccgg
     2941 aagcccatcg cccgctcacg ctcctgccta gccagtacgc tccggtttcc gtgccgagtt
     3001 gcctgaaccc gccgagatca gtctgtaccc tccccctgtc atcctga
//
LOCUS       DASUBI010000742         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1337299, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000742 DASUBI010000000
VERSION     DASUBI010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337299"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU72_14485"
     CDS             <1..184
                     /locus_tag="VFU72_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5424749.1"
                     /translation="ACVVVGGAVAEAGQHLMNPLNARVRQYAYPASMRRARLALSQLG
                     ADATVLGAVALALGHE"
     gene            440..2152
                     /locus_tag="VFU72_14490"
     CDS             440..2152
                     /locus_tag="VFU72_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424750.1"
                     /translation="MQTAEVRPEPPFVGSPEEQQIARDIFEAMRGMGRFFSRTAAIRV
                     SVSSLAEYLAARRPEQDAGAWTAQLLDILTKNRAVFDVEEAGDQLLAITTVAGEAPSR
                     VNRPDTEHMLAQRLTEPTPVTPKQWTSRRQPAPLEELVSPGAETAPADAVAPSPARVE
                     IIPEPVEETLEELDEEEAAPVAAAAAPVATTTANLDELSDEQLAEVISDELRADLTVA
                     NFGHRWMAEDKVPRLSRGDLRRVHDYIVERNEPLGDETLLQDILGVRPGADNYDLMRF
                     ALNYRLSRENKEFEFVGTPDARLWTTTALPAIGTAKRKASEIGQEYRYLLSEPPLEIL
                     PEERVAEHVLTFYEYQYGVLPFNPVFQALLPAALLPDQRAAVLTFEAPQTYETFLVEL
                     RYPTGNRGGYLAGFDRFFLENLVPGALFTIERNAENDGRYLIEYLPVSGETEKLLQVD
                     EKRNRYVFRPRTFYCATSDEMLLTENRFPQLANAAPLEDRSRRRPELALGATFERIGE
                     QVGSSDQPRYMALLDDLLAAANVERPMSAELIRSIVTSGEHPEFSTDSEVTDVFYFQP
                     ERAG"
     gene            2118..3074
                     /locus_tag="VFU72_14495"
     CDS             2118..3074
                     /locus_tag="VFU72_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5424751.1"
                     /translation="MSFTSSPSEQDRPSVEELNEILRAAAAGEHSAARAARLSDLDAS
                     SGRELWRLWQRIPVDARRYLVREMVELSESNVELNFARVLRMALDDPDAEVRALAVSG
                     LWENESNLLLDQFLDLLAVEPEPVVRETLAQALGQFAYRCALAEVDGDRSERVRQALL
                     DLFRSNESIGVRRRALESLAYFDDDEEVEELIAEAYDSLHHDLRVSALFAMGRNLADR
                     WLEPVLEGLEDEEAELRFEAARASGEFGDQRAVDLLLNLVADEDREVQFAAITALGQI
                     GGKLAVQALGRLARSDDEPIAELAREAQTQALQVNDPLRPGL"
     gene            3101..4135
                     /locus_tag="VFU72_14500"
     CDS             3101..4135
                     /locus_tag="VFU72_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5424752.1"
                     /translation="MADGALRLIYSDDQQRWNSAVQCLGGHLLQSWDWGEFKSRHGWR
                     PARLLLSAGEQPLAAAQILFRRVGPFAAGYAPRGPVLAGNDPSLLAALTLGLDRVSRR
                     ERALLTLIEPERRDIDWSQLGGLGWRGSREIVQPRRTIKVSLAGTDEQLLGAMKPKTR
                     YNIRLSERRGVTVRYGCVADLPAFHTLLRETGERQAFGVHDFAYFEDLLRTFGDRGVL
                     LLAEYEGQLAAAALVVRAFDEAVYLYGASCAGLQKHMGAYLVQFEAMRWARAAGCRWY
                     DLWGIPAEDELPEVDKGQIGRDVRGAMLGVYTFKQGFGGETVSYPPMAERVYAGPLVR
                     LARRLGWGTA"
BASE COUNT          688 a         1292 c         1465 g          699 t
ORIGIN      
        1 ggcctgcgtg gttgtcggcg gtgcggtggc cgaagcaggg cagcacctga tgaatccctt
       61 gaacgcgcgg gtcaggcagt acgcataccc agcctcgatg cggcgggcgc ggctggcgct
      121 cagccagctc ggcgccgacg ccacggtgct gggcgcggtg gccctggcgt tgggacacga
      181 gtgacggcgc cggcattgcc gatctggcgg ctcaccgtgt aaacttcgcc gggatgagat
      241 gaaagccccg gggagatcgc catgcaatcc gtccgactcc gtgatgagta tctgacctac
      301 ctcttaatgt tgcttcgcaa cgcgaatcga cctctgacca ccgccgagct ggtgcacgct
      361 ttgcgtgagc gcggccaggg cggttcctaa ttcggaaaac ggcaatcggc caacaggaaa
      421 aagaggagtg acgtcgagca tgcagacggc cgaagtgcgg cccgagcctc cgtttgtcgg
      481 gtctccggaa gagcaacaga tcgcccgcga catctttgag gctatgcgcg ggatgggccg
      541 tttcttttcg cggacggccg cgattcgcgt ctcggtctct tcgctggcgg agtatctcgc
      601 cgcgcggcgg cccgagcagg atgctggagc ctggaccgcg caactcctcg acatactgac
      661 caagaatcgc gctgtcttcg acgtagagga agccggcgac caactactgg cgatcacgac
      721 ggtagccggc gaggcgccca gccgggtcaa ccgccccgac acagagcaca tgctggcgca
      781 acggctgacc gagccgacgc cggtgacccc caagcagtgg acatcacgcc ggcagcccgc
      841 gccgctggag gaactggtct cccccggcgc ggaaacggcc cctgctgacg cggtcgcgcc
      901 gtctccggct cgcgtggaga ttatccctga gccggtggaa gagacgctcg aggagctgga
      961 cgaggaagaa gcggctccgg ttgctgccgc cgccgctcca gtcgcgacca cgaccgcgaa
     1021 cctcgacgag ctgagcgacg agcaactggc cgaggtgatc agcgatgagc tgcgcgccga
     1081 cctgacggtg gccaacttcg gacatcgctg gatggcggag gataaggtac cgcgcctcag
     1141 ccgcggtgat ctgcggcgtg tccacgacta tatcgtggag cgcaatgagc cgctgggcga
     1201 tgagacgctg ctgcaggata ttctgggtgt ccggccgggc gcagacaact acgatctaat
     1261 gcgcttcgcg ctcaactatc gcctctcgcg cgagaacaag gagttcgagt tcgtcggcac
     1321 gcccgatgcg cgcttgtgga cgacaaccgc gctgccggcg atcggcacgg ccaagcgtaa
     1381 ggcgagtgag atcggacagg aataccgcta cctcttgtcc gagccgccgc tggagatctt
     1441 gccggaggag cgggtcgccg agcacgtgct gacgttctat gagtaccaat acggagtctt
     1501 gccgttcaac ccggtctttc aggcgctctt gccggcggcg ctgctgcccg atcagcgcgc
     1561 ggcggtgctc accttcgagg cgccgcagac gtatgagacc ttcctggtcg agttgcgata
     1621 cccgaccggc aaccggggcg gctacctggc cggcttcgat cgcttcttcc tggagaatct
     1681 ggtgccgggc gcgctcttca ccatcgagcg caacgcggag aacgacggcc gctacttgat
     1741 cgagtacctg cccgtttccg gcgaaacgga gaagctgctg caggtcgacg agaagcgcaa
     1801 tcgctatgtc tttcggccgc gcaccttcta ctgcgcgacc tccgacgaga tgctgctgac
     1861 cgagaaccgc ttcccgcagc tcgccaacgc cgctccgctg gaggatcgta gccggcgccg
     1921 gccggagctg gcgctggggg cgacgttcga gcgcatcggc gagcaggtcg gcagcagcga
     1981 tcagccgcgc tacatggcgt tgctggatga tctgctggcc gcggccaacg tcgagcggcc
     2041 gatgagcgcg gagctcatcc gctcgatcgt caccagtgga gagcaccctg aattcagcac
     2101 ggattccgag gtcaccgatg tcttttactt ccagcccgag cgagcaggat agaccgagcg
     2161 tcgaagagct gaacgagatc ttgcgcgcgg cggcggccgg agagcattcg gccgcacgcg
     2221 cggcgcggtt gtccgacctc gatgcatcga gtgggcgaga gctctggcgg ctctggcagc
     2281 gtattcctgt cgacgcccgg cgctatctgg tgcgcgagat ggtcgagctg tcggagtcca
     2341 acgtcgagct gaacttcgcg cgtgtgctcc ggatggcgct tgacgatcca gacgccgagg
     2401 tgcgggcgct cgccgtgagt gggctgtggg agaacgagag caacctgctg ctcgatcagt
     2461 tcttggacct gttggccgtg gaacccgagc cggtggtgcg cgagacgctg gcccaggcac
     2521 tcgggcagtt cgcgtaccgc tgcgccctgg cggaggtgga tggcgatcgc tcggagcgcg
     2581 tccggcaggc gttgctcgac ctctttcgct ccaacgagtc gatcggcgtc cggcgccgcg
     2641 cgctggaatc actggcttac ttcgacgacg acgaagaggt tgaagagctg atcgcggaag
     2701 cctatgactc gctgcaccac gacctgcgtg tgagcgcgct gttcgccatg ggacgtaatc
     2761 tagccgatcg ttggttggaa ccggtgctgg agggcctgga agacgaggag gccgagctgc
     2821 gcttcgaggc tgcccgcgcc tcgggcgaat tcggggacca gcgcgcggtc gacctgctgc
     2881 tgaacctggt ggcggacgag gatcgcgagg tgcagttcgc ggcgatcacg gcgcttgggc
     2941 aaatcggtgg caagctggcg gtgcaggcat tggggcgtct ggctcgctcc gacgacgagc
     3001 ccatcgcgga gctggcacgc gaggcgcaga cgcaggcgct gcaagtgaac gatcctctgc
     3061 gaccgggcct gtgaacgtcg accgtgttgc acgtcatcaa atggccgacg gcgcgctgcg
     3121 actgatctac agcgacgacc aacagcggtg gaatagtgcg gtgcagtgtc tcggcggtca
     3181 cctgttgcag agttgggact ggggcgagtt caagagccgg cacggttggc gacccgcgcg
     3241 gctgctgctc agcgcgggcg agcaaccgct ggcggcggca cagatcctct ttcgccgcgt
     3301 gggcccgttc gcagcgggct acgcgccgcg cggcccggtc ctcgccggca acgatccgag
     3361 cctgctggcg gcgctgaccc tggggctcga ccgcgtgtcg cggcgcgagc gcgcgttgct
     3421 gaccttgatc gaaccggagc gtcgcgatat cgactggtcc cagctgggcg gtctcggctg
     3481 gcgcgggagc cgcgagatcg ttcaaccgcg gcgcaccatc aaggtttcgc tcgctggcac
     3541 cgacgagcaa ctgctgggcg cgatgaagcc caagacccgc tacaacattc gcctgtccga
     3601 gcgccgcggc gtgaccgtgc gctatgggtg cgtggccgat ctgcccgcgt tccatacact
     3661 tctgcgggag accggggagc ggcaggcatt cggcgtccac gacttcgcct acttcgaaga
     3721 cttgctacgc accttcggcg accgcggtgt gctccttctg gccgagtacg aagggcaact
     3781 ggcagccgcg gcgctggtgg ttcgtgcctt cgacgaggcg gtctatctct acggcgcgtc
     3841 ctgcgcgggg ctgcagaagc acatgggagc gtacctggtg cagttcgagg ccatgcgctg
     3901 ggcgcgtgca gccggctgtc gctggtacga cctctggggt atcccggctg aggacgaact
     3961 accggaggtt gataagggac aaatcgggcg ggacgtgcgt ggtgcgatgc tgggggtcta
     4021 caccttcaag caggggttcg gcggcgaaac ggtgagttac ccgcctatgg ccgagcgcgt
     4081 ctacgccggt ccgctggtgc ggttggcgcg gcggctggga tggggcacgg catgagcggg
     4141 catg
//
LOCUS       DASUBI010000743         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_265154, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000743 DASUBI010000000
VERSION     DASUBI010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265154"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFU72_14505"
     CDS             complement(<1..959)
                     /locus_tag="VFU72_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5424753.1"
                     /translation="MAGRPGSFGEQLRRHRETRGLSQEELAERAGLSANAIGALERGE
                     RRRPYPDTLRRLGDALELDESQRAGLAAAVGHAGARVGAGNQAAAPTGLPRDPTPLVG
                     REREAAEVRRLLGRPEVRLLTLTGPGGVGKTRLARHVAAGLTADYPDGVIWVPLGSLN
                     DPALVLPTLARAAGVVETPGRDLRRTLYSVLSARHALVVLDNFEHVLDAAADVAEALL
                     ACPTLDLLATSRAPLGVRGEQEYPLPPLDVPPPSRVLEPEAVAEAASVRLFLQRAREA
                     SPSFALTAENAATVAVICRRLEGLPLALELAAARVKLLTPTQLL"
     gene            complement(1319..2563)
                     /locus_tag="VFU72_14510"
     CDS             complement(1319..2563)
                     /locus_tag="VFU72_14510"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5424754.1"
                     /translation="MPTPNGSDVKKVVLAYSGGLDTSIILAWIKENYGCEVIAYTADL
                     GQGDSFEGLEAKALNSGASKLYVRDIKEELVTDYAFPVLRAGALYERKYLLGTSLARP
                     LTAKHQVEIAKLEGADAVAHGATGKGNDQVRFELTYQALAPNLKVIAPWREWNLRSRE
                     DLLDYAYAHDIPVNQSKTNIYSRDQNLWHLSHEGGGLEDPWNAPNEDMFQLTNHPLDA
                     PDKPVTIVLGFEEGYPVSLDGSPVAPVQLVNLLNELGGEHGIGRIDLVENRLVGMKSH
                     GVYEQPGATILSTAHKELESIVLDRETLHYKDLVAQKYAELVYNGLWFTPLRQALDAF
                     VDVTQKPMSGEVRLQLYKGNVIVTGRRSPNTLYHMDYATFGQDEVYNQADAKGFITLF
                     GLPLKVAALVQQQKEQEQAGEK"
BASE COUNT          416 a          950 c          982 g          443 t
ORIGIN      
        1 agcaactggg tgggcgtgag gagcttcacc cgcgctgccg ccagctccag cgccagtggc
       61 aggccctcca gccgccggca gatcacggcc accgtcgcgg cgttctccgc cgtcagagcg
      121 aatgacggcg acgcctcgcg ggcgcgctgg aggaagagcc gcaccgaggc ggcctcggcc
      181 accgcctccg gctccagcac gcggctggga ggtggcacgt cgagcggcgg cagcggatac
      241 tcctgctccc cgcgcacccc gagcggcgcc cggctcgtcg ccagcagatc gagcgtgggg
      301 caggccagca gcgcctccgc cacgtccgcc gccgcgtcaa gcacgtgctc gaagttgtcc
      361 agcacgacca gcgcgtgccg agcggagaga acggagtaga gcgtccggcg gagatcccgg
      421 ccgggcgtct cgacgacgcc cgccgcgcga gccagcgtcg gcagcacgag cgcggggtca
      481 ttgagcgagc cgagcggcac ccagatgaca ccgtcggggt agtcggcggt cagacctgcg
      541 gcgacgtggc gcgccaggcg cgtcttgccg acgccgcccg gcccggtgag cgtgagcagg
      601 cgcacctccg gccggcccag caggcgccgc acctcggccg cctcgcgctc gcgaccgacc
      661 agcggtgtcg ggtcacgcgg caatcccgtg ggcgcggcag cctggttgcc tgcgcccacg
      721 cgcgcgcccg cgtgcccgac ggcggcagcc agcccggcgc gctgagattc gtccagctcc
      781 agggcgtcgc ccagccggcg cagcgtatcc gggtaagggc ggcggcgctc gccacgctcc
      841 agcgcgccga tcgcgttggc cgagagcccg gcccgctcgg ccagctcctc ctgcgacagc
      901 ccccgcgttt cgcggtggcg tcgtagctgc tcgccgaacg atcccggccg ccccgccacc
      961 ctgcttcctc ccagtgcacc gctcagggcg gaatgatacc cgacccacgt tcgcatcgcc
     1021 gccggactgc ggcgctctgc cagcggagcg gtggcgctcc gtccgcgaag ccgcctgcag
     1081 gggctgagcg tccacgcgcg gggcatgaca acgaagaagg aaggccgtgc cagggaaagc
     1141 gaggggagat cctgaggtcg cggcctcagg atctcccccc tgtgtgtctc gcgccgctgc
     1201 gtcatgtcgt cgctacgcac cgactgtgga atctcagccc ggaaattcta ttccggacgg
     1261 agccgggcga ccccggcgca cgagctgcga tacgaagcgg cccctactcg ccctccgcct
     1321 acttctcgcc tgcctgctcc tgctctttct gctgctggac cagcgcggcg accttcagcg
     1381 gcaggccgaa gagggtgatg aagcccttgg cgtcggcctg gttgtagacc tcgtcctggc
     1441 cgaaggtggc gtagtccatg tggtagagcg tgttgggcga gcgccgcccg gtcacgatca
     1501 cgttcccctt gtagagctgc aggcgcacct cgccgctcat cggcttttgc gtcacatcga
     1561 cgaaggcgtc gagcgcctgg cgcagcggag tgaaccagag gccgttgtag accagctcgg
     1621 cgtacttctg cgcgaccaga tccttgtagt gcagtgtctc gcggtcgagc acgatcgact
     1681 ccagctcctt gtgggcagtg gagaggatgg tggcgcccgg ctgctcgtag acgccgtggc
     1741 tcttcatgcc gaccaggcgg ttctcgacca ggtcgatgcg gccgatgccg tgctcgccgc
     1801 ccagctcgtt gagcaggttg accagttgca ccggggcgac cggcgagccg tcgaggctga
     1861 ccgggtagcc ctcctcgaaa ccgaggacga tggtgaccgg cttgtcgggc gcatccagcg
     1921 ggtggttggt cagctggaac atgtcctcgt tgggcgcgtt ccagggatcc tccagcccgc
     1981 cgccctcgtg gctgaggtgc cagaggttct ggtcgcggct gtagatgttg gtcttgctct
     2041 ggttgaccgg gatgtcgtgg gcgtaggcgt agtcgagcag atcctcgcgc gagcgcaggt
     2101 tccactcgcg ccagggagcg atgaccttga ggttgggggc gagggcctgg taggttagct
     2161 cgaagcggac ctgatcgttg cccttgccgg tggcgccgtg ggccaccgcg tcggccccct
     2221 ccagcttggc gatctcgacc tgatgcttgg ccgtcagcgg gcgcgccagg ctggtgccga
     2281 gcaggtactt gcgctcgtag agcgcgcctg cgcgcagcac cgggaaggcg tagtcggtga
     2341 ccagctcttc cttgatgtcg cgcacgtaga gcttggaggc gccgctgttg agcgccttgg
     2401 cctccaggcc ctcgaaggag tcgccctggc cgaggtcggc ggtgtaggcg atgacctcgc
     2461 agccgtagtt ctctttgatc caggcgagga tgatcgaggt gtcgaggccg cccgagtagg
     2521 cgagcacgac cttcttcacg tcggagccgt tcggcgtggg catgactgct gttctcctct
     2581 tactcgtatg tccaaaagcg cgccggaaat aggggaaagc gaccgctagc gccgcctggc
     2641 gcgtccgggc atacggtaga gcatcggcag gcacacctct cgaacggcgc aaaggccgcg
     2701 atcacgaccc gcagagggta tcaagccgag gaacgtgatg tcaagctctg gaaagtgtca
     2761 taaagagtac ccgattagga cttaatgagc c
//
LOCUS       DASUBI010000744         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_364262, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000744 DASUBI010000000
VERSION     DASUBI010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364262"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="VFU72_14515"
     CDS             <1..1820
                     /locus_tag="VFU72_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5424755.1"
                     /translation="FQADITVPYFGYRFYRGDFYLGYKLAMNLAGLLLILGVLYAFAR
                     RYRERPIWLANSPDDVVLLAFLLVLVAQGFALQALRLAVTQDPWGPWSFVSYPMSLAL
                     RGLPEGWLRGIHAVNWWVHFLTTYVFLSYLVYSKMIHVFTGLANVTFRRLKPLGELEP
                     IPNLEEAESFGIGALADFSWAQLMSADACMHCGRCLQYCPTFNTGKVLRPRDLVLEVA
                     GFMADKGGIFSGELGQAETPGRFRWGRGPDRALIPEVVSPQELWECTTCAACMQQCPV
                     AIEHVPMIVGMRRNLVLEQGEFPPELVNTFNNLERFNSPYAFPPRERDKWTEKLEQPV
                     PRVAERAAEGQEVEVLFFVGCLGSFDARNQRTTIALARLLQTAGINFAILGKDETCCG
                     DPARRIGNEYLAQMMAQQTVETLNEASFKKIVTACPHCFNALLNEYPQIGGNYEVIHH
                     SQLISQLLAEGRLTLDPNSAVARGKVTYHDPCYLGRYNDIFDDPRAVVEALPGADLIE
                     MRRNRKTSFCCGGGGGRLFMEETTGSRINQARVREALDTGAEVLCAACPYCMTMFEDG
                     IRGVGVEETFQVRDIAELVAASLAAPVGAQAGPAAESEN"
     gene            1821..2330
                     /locus_tag="VFU72_14520"
     CDS             1821..2330
                     /locus_tag="VFU72_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5424756.1"
                     /translation="MLDPAAISDEMLAILDRTGAQYHAYRHRPVLSYEDAEAVGREQG
                     WSGTEGKVVVCRADDGYAVYVTRQGERLDQRAMKRALGVRKLRVASADELRAGFAAEP
                     GAAYPFGFAAEVPILVDPAIYDEDWLLFSAALPTVTLRIRGRDLRRLFAGLANHVSEL
                     PSAASGEQG"
     gene            2344..2832
                     /locus_tag="VFU72_14525"
     CDS             2344..2832
                     /locus_tag="VFU72_14525"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5424757.1"
                     /translation="MNEVFRDLYRHNQWANEQLLDACAALDEAQLNAGNPGAYGTIAE
                     TLTHLVGAEQRYLWMLTGQQSDLSVFESEGFPGFERLRAAARQAGAGLTEMAAQAEPG
                     RILRGERRGEPYAIPAEIVLIQVINHATEHRTNVTTILAQLGIEPPEIDGWAFREQVT
                     AG"
     gene            complement(2858..>3590)
                     /locus_tag="VFU72_14530"
     CDS             complement(2858..>3590)
                     /locus_tag="VFU72_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU5424758.1"
                     /translation="PDALLPERLRAVLAVLYLIFNEGYTASAGQALIRADLCREAIRL
                     GRILAHLMPDEPEGLGLLALMLLHDSRRAARTAPDGALVPLEEQDRGAWDGAEIAEGI
                     ALVERALRLRRPGPYQLQAAIAALHAEAGTPRETDWPQIAALYGVLAQLAPSPVVELN
                     RAIALAMAESPRAGLDRLDRPELAAALAGYHLYHAARADLLRRAGSPQAALAAYRQAL
                     ALAASQPERAYLARRLAALEAAALD"
BASE COUNT          532 a         1257 c         1276 g          525 t
ORIGIN      
        1 tcttccaggc cgacatcacc gtgccctact tcggctaccg cttctaccgc ggcgacttct
       61 acctcggcta caagctggcg atgaacctgg ccgggctgct cctgatcctg ggcgtgctct
      121 acgccttcgc ccggcgctac cgcgagcgtc ccatctggct ggccaacagc cccgacgatg
      181 tcgtgctgct ggccttcctg ctggtcctcg tcgctcaggg cttcgcgctg caggcgctgc
      241 ggctggcggt gactcaggat ccctgggggc cgtggtcgtt cgtctcctac cccatgtcgc
      301 tggcgctgcg cggcctgccg gagggctggc tgcgcggcat ccacgcggtc aactggtggg
      361 tccacttcct gacgacctac gtcttcctca gctacctggt ctacagcaag atgatccacg
      421 tcttcaccgg cctggccaac gtcaccttcc gccggctcaa gccgctgggt gagctggagc
      481 cgatccccaa cctggaggag gccgaaagct tcggcatcgg cgcgctggcc gacttctcct
      541 gggcgcagtt gatgagcgcc gacgcctgca tgcactgcgg gcgctgcttg cagtattgcc
      601 cgaccttcaa caccggcaag gtgctgcggc cgcgcgacct ggtgctggag gtggccggct
      661 tcatggccga caaaggcggc atcttctccg gcgagctggg gcaggccgaa acgcccggcc
      721 gcttccgctg ggggcgcggg ccggaccgcg cgctgatccc ggaggtcgtc tcgccccagg
      781 agctctggga gtgcaccacc tgcgccgcct gcatgcagca gtgcccggtg gccatcgagc
      841 acgtgccgat gatcgtgggg atgcggcgca atctggtgct ggagcagggt gaattcccgc
      901 ccgagctggt caacaccttc aacaacctgg agcgcttcaa cagcccctat gccttcccgc
      961 cgcgcgagcg cgacaagtgg accgagaagc tggagcagcc ggtgccgcgc gtggccgagc
     1021 gggcggccga gggccaggag gtcgaggtcc tcttcttcgt gggctgcctc ggctccttcg
     1081 acgcgcgcaa ccagcgcacg acgatcgcgc tggcgcgtct gctccagacc gccggcatca
     1141 acttcgccat cctgggcaag gatgagacct gctgcggcga cccggcgcgg cgcatcggca
     1201 acgaatacct ggcgcagatg atggcgcagc agacggtcga gacgctgaac gaggccagct
     1261 tcaagaagat cgtgaccgcc tgtccgcact gcttcaacgc acttctgaac gagtacccgc
     1321 agatcggcgg caactacgag gtcatccacc acagccagct gatcagccaa ctgctggccg
     1381 agggccggct gacgctggac ccgaactcgg cggtggcgcg cggcaaggtc acctaccacg
     1441 acccttgcta tctagggcgc tacaacgaca tcttcgacga cccgcgcgcc gtggtcgagg
     1501 cgctgccggg cgccgacctg atcgagatgc ggcgcaaccg caagacgtcc ttctgctgcg
     1561 gcggcggcgg cgggcgcctc ttcatggagg agacgaccgg cagccgcatc aaccaggcgc
     1621 gcgtgcgcga ggcgctggac accggcgccg aggttctctg cgccgcctgc ccctactgca
     1681 tgacgatgtt cgaggacggc atccgcgggg tcggcgtcga ggagacgttc caggtgcgcg
     1741 acatcgccga gctggtggcc gcctcgctgg cggcgccggt gggggcgcag gccggtccgg
     1801 ccgcggagag cgagaactag gtgctcgatc cggccgcgat cagcgacgag atgctggcaa
     1861 tcctcgaccg gaccggcgct cagtatcacg cttaccggca ccggccggtg ctcagctacg
     1921 aggacgccga ggcggtcggg cgcgagcagg gctggagcgg caccgagggg aaggtggtcg
     1981 tctgccgggc ggacgacggc tacgccgtct acgtgacccg gcagggcgag cggctcgacc
     2041 agcgcgcgat gaagcgggcg ctgggcgtgc gcaagctgcg cgtcgccagc gccgacgagc
     2101 tgcgcgcggg cttcgccgcc gagccggggg cggcctatcc tttcggcttc gcggcggagg
     2161 tgccgatcct ggtcgacccg gcgatctacg acgaagactg gcttctcttc agcgcggcgc
     2221 taccgacggt gacgctgcgg attcgtgggc gcgacctgcg gcggctcttc gccgggctgg
     2281 cgaatcacgt cagtgagctg ccgagcgcgg ccagcggcga gcagggatga gagggacggg
     2341 cagatgaacg aggtcttccg cgatctctac cgccacaacc agtgggccaa tgagcaactg
     2401 ctggatgctt gcgccgcgct cgacgaggcg cagctcaacg ccggcaaccc cggcgcctac
     2461 ggcacgatcg ccgagacgct gacgcatctg gtcggcgccg agcagcgcta cctctggatg
     2521 ctgaccggcc agcagtcgga cctgtcggtg ttcgagagcg agggcttccc cggcttcgag
     2581 cggctgcgtg ccgccgcgcg acaggccggc gcggggctga ccgagatggc cgcgcaagcc
     2641 gagccggggc gcattctacg cggcgagcgt cgcggcgagc cctacgccat ccccgccgag
     2701 atcgtgctga tccaggtgat caaccacgcc accgagcacc gcaccaacgt caccaccatc
     2761 ctggcccagc tcggcatcga gccgccggag atcgacggct gggcgttccg ggagcaggtg
     2821 accgccggct aaaccgcctt ccgcgccggg ctgccgctca gtccagcgcg gcggcctcca
     2881 gcgcggccag gcggcgcgcg agataggcgc gctcgggctg gctggcggcg agcgccagcg
     2941 cctggcgata ggctgccagc gccgcctgag gcgagcccgc gcgtcgcagc aggtccgcgc
     3001 gcgccgcgtg gtacaggtgg tatccggcca gcgcggcggc cagctccggg cggtcgagcc
     3061 ggtcgagtcc cgcgcgcggg ctttcggcca tcgccagcgc gatggcgcgg ttcagctcga
     3121 cgaccggaga gggcgcgagc tgggcgagca ccccgtagag ggcggcgatc tgcggccaat
     3181 cggtctctct gggagtaccg gcctcggcgt ggagcgccgc gatcgcggcc tgcaactggt
     3241 agggtccggg gcggcgcagc cgcagggcgc gctccaccag cgcgatccct tcggcgatct
     3301 cggcgccgtc ccaggcgccg cggtcttgct cctccagcgg caccagcgcg ccgtccggcg
     3361 cggtccgtgc cgcgcgccgg gaatcgtgga gcagcatcag cgcgagcaag cccagccctt
     3421 ccggctcgtc cggcatcagg tgagccagga ttcgtccaag ccggattgct tcccggcaaa
     3481 ggtcggcgcg aatcagcgcc tggccggcgc tggctgtgta accctcgttg aagatgagat
     3541 agagcaccgc cagcacggcc cgcagccgct cggggagcag tgcgtccggc
//
LOCUS       DASUBI010000745         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_183995, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000745 DASUBI010000000
VERSION     DASUBI010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183995"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFU72_14535"
     CDS             complement(<1..537)
                     /locus_tag="VFU72_14535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5424759.1"
                     /translation="MTSPAPVGSVLSLFWTPLVAVGATDGTRPNAQISVSTFGAGVVP
                     DRPRIVCVLYKANYTHGLVLARRNFSVSVLAEDQIDLIPALGFASGRDRDKLDGLDYT
                     LTQRANPVFTGCTGWLECEVIETADFGDSTAFLGAVLQHERLREGYGLSWSRLAPSLP
                     RQWLETWNAKLAGDIVKYR"
     gene            complement(545..1060)
                     /locus_tag="VFU72_14540"
     CDS             complement(545..1060)
                     /locus_tag="VFU72_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5424760.1"
                     /translation="MAKRAVVPLDRSDRAELALQAIPKLLDPEDEIVLLSIAAPKSSV
                     PRGTRPGRAAWFSSGPQGEMIAGRDPDFVTYAETEDQVIQGQLAEMEDYLHNKVNELQ
                     KQGFVHTKLVCEVSDDAAGTIADLARRVDPTFIIMVRTTHPDIGTRVFGTVAQHLIRE
                     DIAPVVILPAK"
     gene            complement(1165..>2077)
                     /locus_tag="VFU72_14545"
     CDS             complement(1165..>2077)
                     /locus_tag="VFU72_14545"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424761.1"
                     /translation="GPGGHFVAGARAGGNATAKAVEGGYVINGRYRYSSGVPYSTHHL
                     VHVRIEGAHPGGPPDGTLCWAVVPRDQYEILWDWGNFNDLGLQASGSNTVEMKDAWIP
                     KHFLVPDNFRGYDQTNGTPGTRLHGNPMYLGSHAAMYHAGLVGPQIGAARAALDEFED
                     VLRNTRNRMGPPILQMEDRGFQTAFGLALAQVDAAEMILIRVGEMTGEYCERWARTGR
                     PFTPEEDTRLYAALQQAGILAWNAMEQVWNSAPVDAAKAGARLQRYYRDISMYRLHVS
                     ARPLTLAPRLAQMYFGIVPEAPPAPQA"
BASE COUNT          330 a          663 c          732 g          352 t
ORIGIN      
        1 gcggtacttc acgatatcgc cggccagctt cgcgttccag gtttcgagcc actggcgcgg
       61 cagcgacggc gccaggcggg accagctcag cccgtagccc tcacgcaaac gctcgtgctg
      121 cagcacggcg ccgagaaacg ccgtcgagtc gccgaagtcg gccgtctcga tcacctcgca
      181 ctccagccag cctgtacagc ccgtaaacac cgggttggct cgctgcgtca gggtgtagtc
      241 gaggccgtcc agcttgtctc ggtctcgtcc ggaggcgaag ccgagcgccg ggatcagatc
      301 gatctggtcc tcggcgagca ccgagacgct gaagttgcgc cgggcgagca cgaggccatg
      361 cgtgtagttg gctttgtaga gcacgcagac gatgcggggg cggtccggca cgacgccggc
      421 tccgaaggtc gagacggaga tctgggcgtt cggccgcgtg ccgtcggtgg cgcccaccgc
      481 gaccagcgga gtccagaaca ggctgaggac ggagcctacg ggagctggcg acgtcatgcg
      541 actgctactt cgcgggcaga atcaccacgg gcgcgatgtc ctcgcggatc aagtgctggg
      601 ccacggtgcc gaacacgcgc gtgccgatgt ccgggtgggt ggtacgcacc atgatgatga
      661 acgtggggtc gacgcggcgg gcgaggtcgg cgatggtccc cgcggcgtcg tcgctgacct
      721 cgcagaccag cttggtgtgc acgaaacctt gcttttgcag ctcgttgact ttgttgtgga
      781 ggtagtcctc catctcggcg agctggccct ggatcacctg gtcctcggtc tcggcgtagg
      841 tgacgaagtc ggggtcgcga ccagcgatca tctcgccctg cggtcccgag ctgaaccagg
      901 cggcgcggcc gggtcgcgta ccgcgcggca ccgagctctt cggcgcggcg atcgagagca
      961 agacgatctc gtcttcggga tcgagcagct tcggaatggc ctgaagggca agctcagcgc
     1021 gatcgcttcg gtcgagaggg acgactgctc gtttagccat gactttgcct ttccccgata
     1081 gtgccgcgcc ggcggtccag tggcgctaat tctagcgatt cagaaaagta ggaaaagggg
     1141 cgtgagggcg gcgaaaccgg cgcgtcaggc ctggggcgcc ggtggagcct cgggcacgat
     1201 gccgaagtac atttgcgcga ggcgcggcgc cagcgtcaag ggccgtgccg agacgtgcag
     1261 tctgtacatc gagatgtcgc ggtagtagcg ctgcaatcgg gcgccggcct tcgccgcgtc
     1321 gacgggcgca gagttccaca cctgctccat cgcgttccac gccaggatgc cggcctgctg
     1381 cagcgccgcg tagagacgtg tgtcctcttc cggcgtgaac ggacggccgg tgcgggccca
     1441 acgttcgcag tattcgcctg tcatttcacc gacacgaatg aggatcatct ccgccgcatc
     1501 gacctgggcc agcgccaggc cgaacgccgt ctggaagccg cggtcctcca tctgcaggat
     1561 cggcgggccc atgcggttgc gcgtgttgcg cagcacgtcc tcgaactcgt cgagagcggc
     1621 gcgggcagcc ccgatctgcg gcccaaccag accggcgtgg tacatcgccg cgtggctgcc
     1681 gagatacatc gggttaccgt gcaggcgcgt gccgggcgtg ccgttcgtct ggtcgtagcc
     1741 gcggaagttg tcgggcacga ggaagtgttt cggtatccag gcgtccttca tctcgaccgt
     1801 gttggagccg ctggcctgga gaccgaggtc gttgaagttg ccccagtccc agagaatctc
     1861 gtactgatcg cgcggcacca cggcccagca cagagtcccg tcgggcgggc cgcccgggtg
     1921 ggcgccctcg atgcgcacgt gtaccagatg atgcgtcgag tagggcacgc cggaggagta
     1981 gcgatagcgg ccgttgatca cgtagccgcc ctcgacggcc tttgccgtgg cgttgccgcc
     2041 tgcccgggcc ccggccacga agtgcccgcc tggaccg
//
LOCUS       DASUBI010000746         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_919191, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000746 DASUBI010000000
VERSION     DASUBI010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919191"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFU72_14550"
     CDS             <1..400
                     /locus_tag="VFU72_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424762.1"
                     /translation="AYHIPGRAEATTLEIVPEQLPGGEIITVPVRKGGVLLFTNRTPH
                     RSIDNISDVVRWSIDLRFQSASLPTNYRLPDGQPLHEQHDDEPFACYPPEADLLVRSR
                     AQADVVVHDWRQFEQIRRGYVPAPAPRRWD"
     gene            complement(733..1497)
                     /locus_tag="VFU72_14555"
     CDS             complement(733..1497)
                     /locus_tag="VFU72_14555"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU5424763.1"
                     /translation="MRANAMKAALAAGGWSAGAIVPVADPALVEVLALAGFDHVLIDA
                     EHGHISVADCEQLVRAAEAAGITPLVRVPVNAPEQILRYLDTGAQGVLAPQVTTPEDA
                     ARAVAAARYHPRGRRGLAGTRAADYGLRDALGDYARRANDALLVLALIEDVRALDHLP
                     AILAVDGLDAITIGPADLSQSLGHPGERDHPAVQAAIATIVAQGVAAGKVVGMNAPTG
                     LDARRERKRGVRLTSVSVFGLLAQGAQAYLAAARGD"
     gene            complement(1588..2580)
                     /locus_tag="VFU72_14560"
     CDS             complement(1588..2580)
                     /locus_tag="VFU72_14560"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5424764.1"
                     /translation="MAGFFPAGETPAFLWLDGRLVPWADATLHMTAMGASAHVAVFEG
                     IRAYAAGRPDAALLVFRLREHLDRLLDSMTMMRMPLVYSAEELRDAILMLLRANDARG
                     DTYIRPVAFYSGLAHPSFGDTLGAPPQVLIWHRPTGTQLLTERGKHIGVSSWTRLADN
                     VMPPRIKSMSNYQNNRLAQLEARVNGYDDTVMLTAAGKVAESSGACVFLVRHGAVVTP
                     AVTSGILESITRDAVIRLCREELGVPVVEREVDRTELYVADEVFCCGTAAEITPIWSV
                     DRIPLRSNGTGPLTRRLDRLFFDVVRGHHPGYAAWRDGVGRRQGAGGSGAAGTA"
     gene            complement(2919..>3297)
                     /locus_tag="VFU72_14565"
     CDS             complement(2919..>3297)
                     /locus_tag="VFU72_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5424765.1"
                     /translation="FEYGGRPVAYDVAMAAGIAYVDRLGVPAIAAHARALAAALRDGL
                     GRVPGACLCSPDDPALAAGIVTVALDGVDGVTAAAVLRERWRIVTRAALRGTSVRVSL
                     APFVEEGDVACLLESLATLAAGR"
BASE COUNT          506 a         1182 c         1158 g          451 t
ORIGIN      
        1 ggcgtatcac atcccgggcc gcgcggaggc gacgacgctc gaaattgtcc ctgaacagct
       61 ccctggcgga gaaatcatca ccgtgcccgt gcggaagggc ggcgtcttgc tcttcaccaa
      121 ccgaactccc caccgctcga ttgacaacat ctccgacgtg gtgcgctgga gcattgatct
      181 gcgcttccag tcggccagcc tgccgacgaa ttaccggttg cccgatggtc agcccttgca
      241 cgaacagcac gatgacgaac cgttcgcctg ctatccgccg gaggccgacc tgctggtccg
      301 cagccgcgcg caagcggacg tggtcgtgca tgactggcgg cagttcgagc aaatcaggcg
      361 cggctatgtg cccgcgccgg cgccgcgccg ctgggattaa tgcagccaga gaacgctcct
      421 ggtcagagca cgagcagcca cgcggacgcg gatcgctgtt gacctgggta gcgaagtccc
      481 gcagtggcgt tagtcgtcgc ccacacacgc ggtgagcatg acccgggaac agaccagtgc
      541 gatggggcta acacgccgtg aggccgcgcg gcgtccaccg cgatgaccat gccatcaggt
      601 ggcgcagtgg tggcaactac cacgagccgc gccgtgatga gcactggcgg agcgaacaga
      661 ctaccgctag cggacgggtg ttgcccgcgg ctgcggtatt gcagcccccg caccggccgg
      721 ccggtggccg ggtcaatcgc cccgcgccgc tgcgaggtag gcttgcgcgc cctgggccag
      781 caggccaaag acgctgacgc tggtcaggcg cacgccgcgc ttacgctcgc gccgcgcgtc
      841 caaccccgtc ggcgcgttca tccccacgac cttgccggcc gcgacgccct gcgccacgat
      901 cgtcgcgatt gccgcctgca cggccgggtg gtcgcgctca ccggggtggc cgagcgactg
      961 cgagaggtcg gccgggccga tggtaatcgc gtcgaggcca tccacggcga gaatcgcggg
     1021 caggtggtca agcgcccgca cgtcctcgat cagcgccagc accagcagcg cgtcgttggc
     1081 ccggcgagcg tagtcgccaa gcgcgtcgcg caggccgtag tcggccgcgc gcgtccccgc
     1141 caggccgcgc cgcccgcgcg ggtgatagcg cgcggcggcg accgcccgcg cggcgtcctc
     1201 gggcgtcgtg acctgcggcg ccaacacgcc ctgcgcgccg gtgtcgaggt agcgcaggat
     1261 ctgctcgggc gcgttgaccg gcacgcgcac cagcggcgta atgcctgctg cctcggccgc
     1321 gcggaccagc tgctcgcagt cggccacgct gatgtggcca tgctcggcgt caatcaggac
     1381 gtggtcgaac cccgccagcg ccaggacctc gaccagcgcc gggtcggcca ccggcacgat
     1441 cgccccggcg gaccagccgc ccgccgccag cgcggccttc atcgcattcg cgcgcattgt
     1501 tcactccgct cgctgcaaaa cgtcggacac cggatccagg aagcgcgctc ggccttggcc
     1561 ggcattcggg aatgcgcgcc ggttaggcta cgccgtgccc gctgccccac tgccgccggc
     1621 tccctgccgc ctgccgacgc cgtcccgcca cgcggcgtag ccggggtggt ggccgcggac
     1681 gacgtcgaag aagaggcggt cgaggcggcg ggtgagtggt ccggtgccgt tggagcgtag
     1741 cgggatccgg tctaccgacc agatcggcgt gatctcggcg gcggtgccgc agcagaagac
     1801 ttcgtcggcc acgtagagtt cggtgcgatc cacctcgcgc tcgacgaccg gcactcccag
     1861 ctcctcacga cagaggcgga tgacggcgtc gcgcgtgatg ctctccagga tgccgctcgt
     1921 cacggccggc gtgaccaccg cgccgtggcg caccaggaag acgcaggcgc cggaggactc
     1981 ggcgaccttg ccggcggcgg tcagcatgac cgtgtcgtcg tagccgttga cgcgggcctc
     2041 cagctgggcc agccggttat tctggtagtt cgacatgctc ttgatgcgcg gcggcatcac
     2101 gttgtccgcc agccgcgtcc acgagctgac gccgatgtgc ttgccgcgct cggtgaggag
     2161 ctgcgtgccg gtgggccggt gccagatcag cacctggggc ggcgcgccga gcgtgtcgcc
     2221 gaacgagggg tgcgccagcc cactgtagaa ggcgaccggg cggatatacg tgtcgccgcg
     2281 cgcgtcgttg gcgcgcaaga gcatgaggat cgcgtcgcgt aattcctccg ccgagtagac
     2341 cagcggcatc cgcatcatcg tcatcgagtc gagcagccgg tccaggtgct cgcgcaggcg
     2401 gaagaccagg agcgcggcgt cggggcggcc ggcggcgtag gcgcggatac cctcgaagac
     2461 ggcgacgtgc gcgctggcgc ccatcgcggt catatggagt gtggcgtcgg cccaggggac
     2521 tagccgccca tcgagccaca aaaaggccgg ggtctcgcca gcgggaaaga agcctgccat
     2581 gcgtctccct cgtaatgcgt caccgcgtgg cccacggacc gcccgcgcgc cggtgcggcg
     2641 gcaagcggct acggtgtgcc cgacgacggc gtgggctggt gggcactgac tagccagtgc
     2701 cctggcgcgg tggctgtacg caccaaccta gcatgacctc cccagagcgt caaccacgcg
     2761 cggcaccggg gccagccgcg ccgcgaccgc cgcgccgacg gctattgacg gcagtgaacg
     2821 agggggccgg tcattcactc ctatttccga cctgatcctg attgctgacc gccctggcgc
     2881 catagcccct tctgccgtgc gccatgcgag accctagatc agcggccggc ggccaacgtc
     2941 gccaggctct caagcaggca tgccacgtcg ccttcctcaa cgaacggcgc cagggagaca
     3001 cgcacgctcg tgccgcgcag cgccgcgcga gtcacgatgc gccagcgctc gcgcaggacg
     3061 gctgcggccg ttacgccgtc caccccatcg agcgcgacgg tgacaatgcc cgccgcgagc
     3121 gccgggtcgt ccgggctgca caggcaggcg cccggcaccc gacccaggcc gtcgcgcaag
     3181 gccgcagcca gggcgcgcgc atgggcggcg atcgccggca cgccgagacg gtccacgtag
     3241 gcgatgcccg ccgccatggc gacgtcgtag gcaacgggcc ggccaccgta ctcgaag
//
LOCUS       DASUBI010000747         6880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_327295, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000747 DASUBI010000000
VERSION     DASUBI010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327295"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFU72_14570"
     CDS             <1..639
                     /locus_tag="VFU72_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5424766.1"
                     /translation="LTATLVREDGREREVFALVGPPAYSAPWRELERQGWIAQVDCVE
                     VRVRPPNGREATAPRLLAAKLAAARALLARHPDSPALLVAHRLQEVAALAHACDAPVV
                     TGRTPPQERTRLYDAFRRGEIARLAVSRVANVGVDLPGAALLVQLSGAFGSRQEEAQR
                     VGRLLRPKEDGERAVFYSLVGAGTREQEFAARRQRFLVDQGYRYRVVEAGER"
     gene            934..3399
                     /locus_tag="VFU72_14575"
     CDS             934..3399
                     /locus_tag="VFU72_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5424767.1"
                     /translation="MNHRGALPVSFPSGNRMPQRLRRWRLRHAIGAAELAHWWPLSLS
                     RRIVVTVTLGLGFILLLSGVLVEWTIRESTRAAFDDRVRLAQDFAQRVDDVVISAQAS
                     IEDESAHLQIDPNQGLTAAAQQRLTELLARDNGFNRVVITDVNGRIWWQGSSPHGALD
                     APLDPLAAHMALDTRQTQRGLCSSSQSSIAPLVCFAVPLSDSTGATSAVLAAVFDPTA
                     VPTYSLFTAAAFTPTTTDGINVGLFDTAGSSPIPTSGSPSLFGAEHRRLLAPFIGTAS
                     AGYAIHRPVSGPMHVVVYAPVPTLPSWGVVLEEPRDHVVTLPDQLRQRLALFGAVVLV
                     LAMGLAWTDVRRVVGPLRHLTAAAEQFAAGHLEQPIRLNRGDELGILAGAFETMRLRL
                     RASLNEVETWNQELEQRVARRTSEVEARNHELASLNALAETVGGSLEPREMLEGTLAH
                     VLAITGADAGYCLMPSGDEGQLRLAAGWPGQPPISGGCPPLPGCACRQAFQENRLVQS
                     CAGDAASGGVLCPLMAFPSRLALPLPAGERVQGVLLLGWAAPPSSLREREATLSAIGR
                     QVGMALANARLYEDLQLRDRERLTLLQRLMNAQEDERRRLAQELHDETSQALASLQFG
                     LERLVTGPESSEAVRVVARELRGVTNQTLADVHRLSVELRPSVLDDIGLPAAVERYLR
                     DFGARYGITVDFAAVGIDQVRLFPAAETAIYRIVQGALTNVAQHAQAGHVSLLLRRRG
                     ETFVVVVEDDGRGFDLDAVRAGPLEHQLGLAGMEERAALLGATVTIETAPGMGTTIFV
                     EVPLDLNRSEGRTGDGTAAHSAS"
     gene            3371..4030
                     /locus_tag="VFU72_14580"
     CDS             3371..4030
                     /locus_tag="VFU72_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424768.1"
                     /translation="MARLRILLADDHGVLRAGLRALLDTQPDMRVVGEVGTANEAVEA
                     VAVSHPDLAILDVRMPGGGLDAARRIKQEGQGVKVLILSQYDDPAYLHRALSAGASGY
                     ALKHASAEELLHAIRAIARGEVYLHPALARVLVEESWGPRPSPSAETDQQPSEREAQV
                     LRLIALGYTTRQIADELALSVRTIETYKQRLVEKLGLRGRAALVSYALEHGLLDGEAE
                     R"
     gene            4188..5057
                     /locus_tag="VFU72_14585"
     CDS             4188..5057
                     /locus_tag="VFU72_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5424769.1"
                     /translation="MRGLTSDDPKSDSGSPVSAGGGRLPGADPDHESGSAHPFSRRRF
                     VRLAAVAGLTGAAATGVSFAVGGNAPAAAQAPQHQWAFVIDLRKCDGCEKCTKACQKT
                     HSLAKDQTWIKVYPMSHANGDQYFMPRLCMHCENPPCLRVCPVGATFRNAEGVVLVDQ
                     TKCIGCRACMAACPYEARYFNWTQPPAAPTLKEQPMPEFPVPQIKGTVGKCILCVHNT
                     DVGKLPACVENCSMGALYIADMKADAAVNGLGETVKLSAFLQENDAVRFREELGTRPR
                     VYYILGHGQDLSY"
     gene            5140..6486
                     /gene="nrfD"
                     /locus_tag="VFU72_14590"
     CDS             5140..6486
                     /gene="nrfD"
                     /locus_tag="VFU72_14590"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5424770.1"
                     /translation="MPELAMVREAALRPLGRSGWAFRGLLTLLSLIVLAGLAAFVIQL
                     RGGLGAAGYDDHGFWGIYEANLVAFIGVSYGGALVSAILRLTQARWRAPITRLAEAMA
                     VCSLMVGALFAIIHLGRPDRIWRLLVTPRASSPIVWDFVAVTSYLVATLIFLYLPLIP
                     DIAVLRDRYAHRRGPRARIYRWLALGWRGLAAQRGRLEAGVTMIAILIIPLAVLVHSV
                     LSWAFAVNSRPGWGSTIFAPYFVVAALYSGVGMVILVVAGFRRGYRLERFIGVRHFRY
                     LAYLMLALDLLYLYFTFTEMLTEGYTLNEEITPILTAVLVGQYAPFFWLFVVGAGLIP
                     LLLVALPMTRTVPGIVVAAALVVIGMWVKRLVIVVPAAAQPMIAGAWGTFHPTWVAIA
                     ITAGGAAAVPLLLMLFFKFFPILAIYEIEELAATPAPGPAELANAQRAGAEKGLVG"
     gene            6483..>6880
                     /locus_tag="VFU72_14595"
     CDS             6483..>6880
                     /locus_tag="VFU72_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424771.1"
                     /translation="MRLRSMLVLAVILGGSLLVAFPDGGALAASPPPAAARLALATTP
                     LLDHKGHVIPGKYLVVATLTAADGKPLSNQSITFFEQQEFLGTMRQVGLGMATTDATG
                     TAAVAYQPAQAGAHALAARFVGDAQVAAADA"
BASE COUNT          960 a         2350 c         2418 g         1152 t
ORIGIN      
        1 ctgacggcca cgctggtgcg cgaggacggt cgcgaacgtg aggttttcgc cctggtcggg
       61 ccgcccgcct actcggctcc ctggcgcgag ctggagcggc agggctggat cgctcaggtg
      121 gactgcgtcg aagtgcgcgt ccgcccgccc aacgggcgcg aggcgaccgc gccacggctg
      181 ctggcggcta agctcgcggc cgcgcgtgcg ctgctggcgc gccaccccga ctcgccggcg
      241 ctgctggtcg cgcaccggct gcaggaggtc gccgcgctgg cccacgcctg cgatgcaccg
      301 gtggtaaccg gccggacgcc gccgcaggag cggacgaggc tctacgacgc cttccggcgg
      361 ggtgagatcg ctcggttggc cgtctcgcgg gtggccaacg tgggcgtcga tcttcccgga
      421 gcagccctgc tggtgcagct ctccggcgcg ttcggctcac gccaggagga ggcgcagcgc
      481 gtcgggcggc tgctgcggcc caaggaggac ggcgagcgcg ccgtcttcta cagcctggtc
      541 ggcgccggca cacgcgagca agagttcgcc gcccgccgcc agcgcttcct ggtcgaccag
      601 ggctatcgct atcgggtggt cgaggcgggg gaacgatagg cagcccgtcg ccgggtgtga
      661 gatgcgcgcc gtgggccggc gcggggcggc agtgagcatg gcagttggcc atagcgcggt
      721 caacgtcgtc tacgtgcccg ccgggacgcc tgtcaccgat ttgtaacact ggtagagggc
      781 cgcaccatgc ggctgtaaca tcggccggag tagcgtctcc gtaagacggt ggacgacccg
      841 gcgtgcccga tccggcctgt ctcttgggat tgccggttga agcctcccgt cgtggacccc
      901 ggccggtctc cttctcggtg agggtgcagg cggatgaacc accgcggcgc gttgccggtc
      961 tcgtttccaa gcggcaaccg gatgcctcag cggctgcggc gctggcgttt gcgccacgcg
     1021 atcggcgcgg ccgagctggc tcactggtgg cccctgtcgc tgagccggcg tatcgtcgtg
     1081 acggtcacgc tcggacttgg gttcattctg ctactctccg gtgtcttggt cgagtggacc
     1141 attcgcgaga gcacacgagc ggcgttcgat gatcgcgtgc ggttagcgca ggacttcgcg
     1201 cagcgcgtgg atgacgtggt catctcggcg caggcgtcga tcgaggacga gtccgcgcac
     1261 ctgcagatcg atcccaacca gggattgacc gcggccgcgc agcagcggtt gacggagctc
     1321 ctcgcgcggg acaacggctt caaccgcgtc gtgataaccg acgtcaatgg gcgaatttgg
     1381 tggcagggat cgtccccgca cggcgccctc gacgcgccac tggatccatt ggccgcgcac
     1441 atggcgctcg acaccagaca gacccagcgc gggctctgct cttcttccca gtcatccatc
     1501 gccccgttgg tctgcttcgc ggtgccgttg tcggattcga cgggcgccac cagcgcggtc
     1561 ctggcggctg tcttcgatcc caccgcggtg ccgacctact cgctctttac ggcggccgcc
     1621 ttcacgccta cgacgactga tgggatcaac gtcggcctct tcgacaccgc cggaagctcg
     1681 ccgatcccga cgagtggttc gccctccctg ttcggcgcgg agcatcggcg cttgctggcg
     1741 cccttcatcg gcaccgcctc ggcgggctat gccatccacc ggccggtctc ggggccgatg
     1801 cacgtggtgg tctacgcccc ggtgccgacg ctcccatcgt ggggcgtcgt gctggaggaa
     1861 ccgcgggacc acgtcgtgac cctgcccgat caactccggc agcgcctggc gctgttcggc
     1921 gcggtggttc tggtcctggc gatgggtctc gcctggactg acgtgcgtcg cgtcgtcggt
     1981 ccgctgcgcc acttgacggc ggcggcggag cagttcgccg ccggccacct cgagcagccg
     2041 atcaggctga accggggcga tgagctgggg attctggcgg gtgcgttcga gacgatgcgg
     2101 ctacggctgc gcgcctccct gaacgaagtc gagacctgga accaagagct ggagcagcgc
     2161 gtcgccaggc gtaccagcga ggtcgaggca cgaaaccacg aactggcaag cctcaatgcg
     2221 ctggccgaaa ccgtcggcgg ttcgctcgag ccgcgcgaga tgctggaggg cacgctggcg
     2281 cacgtgctcg cgatcaccgg cgcggacgcg ggatactgcc tcatgccatc cggcgatgag
     2341 gggcagctcc ggctggctgc cggctggccc ggccagccgc cgatctccgg cggctgccca
     2401 ccgctccctg gctgcgcctg ccgtcaggcg ttccaggaga accgcctggt ccagtcctgc
     2461 gccggcgacg cggcgtccgg cggagtcctc tgtccgctga tggcgttccc gtcgcgtctg
     2521 gcactcccat tgccggcggg agagcgcgtg cagggggtcc tgctcctggg ctgggcagcg
     2581 ccgccgtcga gcctgcgtga acgcgaggct acgctgtcgg ctattggccg acaggtgggg
     2641 atggcgctgg ccaacgcgcg gctctacgag gatctccaac tgcgggaccg ggagcgtctt
     2701 acgctcctgc agcggctgat gaacgcccag gaggatgagc ggcggcgact ggcgcaggag
     2761 cttcacgatg aaaccagcca ggcgctggcg tcgctgcaat tcgggttgga gcggctggtc
     2821 acgggaccgg agagctccga ggcagtacgt gtagtggcgc gcgagcttcg cggggtcact
     2881 aaccagacgc tcgccgatgt acaccgcttg tccgttgagc tgcgtcccag cgtactcgac
     2941 gacatcggac tcccggccgc cgtcgagcgc tacctgcgcg atttcggcgc gcgctacggt
     3001 atcaccgtcg acttcgccgc ggtgggcatc gatcaggttc gcctcttccc ggccgcggag
     3061 acagccatct accgcatcgt ccagggcgcc ttgacgaatg tggcgcagca cgcccaggcc
     3121 ggtcacgtga gcctgcttct gcgccggcgt ggggagacgt tcgtggtggt ggtcgaggac
     3181 gacgggcgcg gcttcgatct cgacgcggtg cgcgccggac cgcttgagca tcagctcggc
     3241 ctggcgggga tggaagagcg ggccgcgctg ctcggtgcca ccgtaacgat cgagaccgca
     3301 cccgggatgg gcacgacgat cttcgtcgag gttccactgg acctcaaccg ctccgagggg
     3361 agaactggtg atggcacggc tgcgcattct gctagctgat gatcacggcg tgttgcgggc
     3421 cgggctgcgt gcgctgctcg atacccagcc cgacatgcgg gtggtcggcg aggtggggac
     3481 ggccaacgag gccgtcgagg cggtggctgt gtctcatcct gatctcgcga tcctcgatgt
     3541 ccggatgccc ggaggtggcc tcgatgcggc gcggcggatc aagcaggagg gccagggtgt
     3601 gaaggtactg atcctgagcc agtacgacga cccggcctac ctgcatcgtg ccctctcggc
     3661 gggcgcatcc ggctacgcgc tcaagcacgc cagcgccgag gaattgctgc acgcgatccg
     3721 cgcgatcgcg cggggcgaag tctatctgca tccggcgctg gcccgcgtgc tggtcgagga
     3781 gtcctggggg ccacggccct cgccaagcgc cgagaccgat cagcagccga gcgagcggga
     3841 ggcgcaggtg ctgcgcctca tcgcgctcgg ctacaccacg cgacagatcg ccgacgagct
     3901 cgcactgagc gtccgcacga tcgagaccta taagcagcgg cttgtcgaga aactgggcct
     3961 gcgcggccgc gcagccctag tatcctacgc gctcgagcac ggcctactcg acggcgaagc
     4021 ggagcgctag cgctcgctcg ccccccgctc agactgcacg ctttccacga cacggtcacc
     4081 ccagtccgcg tgctttccac gacgaatacg cggagtcacc gcacgtttcc acgctccgtg
     4141 ctgcccccct cccggaccca gaatgggtga cggtaggagg tggcactatg cggggcttga
     4201 cgagcgacga tcccaagagc gacagcggat ctccggtttc cgccggtggc gggaggcttc
     4261 cgggcgcgga cccggatcac gagtccggct cggcgcatcc cttctcgcgc cggcgcttcg
     4321 tgcggctagc ggccgtcgcc ggcctgaccg gcgccgcggc taccggcgtg tcattcgcgg
     4381 tgggcggcaa cgcgcccgcg gcggctcagg caccgcagca ccagtgggcc ttcgtgatcg
     4441 acctccgcaa gtgcgacggc tgcgagaagt gcaccaaggc ctgtcagaag acgcattctc
     4501 tggcgaaaga tcagacctgg atcaaggtct accccatgag ccatgcgaat ggcgatcagt
     4561 acttcatgcc ccgcctctgc atgcactgcg agaacccgcc ctgcctgcgc gtctgcccgg
     4621 tcggcgcgac cttccgcaac gccgagggcg tggtgctggt ggaccagacg aagtgcatcg
     4681 gctgccgcgc ctgcatggcg gcctgcccct acgaggcccg ctacttcaac tggacccaac
     4741 cccccgccgc gcccacgctc aaggagcaac cgatgccgga gttcccggtg ccgcagatca
     4801 agggcaccgt cggcaagtgc atcctgtgcg tgcacaacac cgatgtcggc aagctgcccg
     4861 cctgtgtcga gaactgctcg atgggcgcgc tctacatcgc cgacatgaag gcggacgcgg
     4921 cggtcaatgg gctcggcgag accgtcaagc tgtcggcgtt cctgcaggag aacgacgccg
     4981 tccgcttccg cgaagagctg ggcacgcggc cgcgggtcta ctacatcctc ggccacggcc
     5041 aggacctcag ctactagatc gatcggcggc ggccgcgcct gccggcctcg cgcggaccgc
     5101 cacacggagg tacggagagc ggtgatgaac gatcgggaag tgcctgagct cgcaatggta
     5161 cgtgaggccg cgctgcgccc gctcggacgc agcggctggg ccttccgggg tctgctgacg
     5221 ctcctgtcgc taatcgtcct ggcgggcctc gccgcgttcg tgatccagct tcgtggcggg
     5281 ctgggcgcgg ctggttacga cgaccacggc ttctggggga tctacgaggc caatctggtg
     5341 gcgttcatcg gcgtcagcta cggcggcgcg ctggtgtccg ccatcctgcg cctcacccag
     5401 gcgcgctggc gagcgccgat cacccggctg gccgaggcga tggcggtgtg ctcgctgatg
     5461 gtcggcgcgc tcttcgccat catccacctc ggccgtccgg atcgcatctg gcggctgctc
     5521 gtcacccccc gcgccagttc accgatcgtc tgggatttcg tcgcggtgac gagttacctg
     5581 gtcgccacgc tgatcttcct ctacctgccg ctgatcccgg atatcgccgt gctgcgagac
     5641 cgctacgcgc accggcgcgg tccgcgcgcg cgcatctatc gctggttggc gctcggctgg
     5701 cgcgggctcg cggcgcagcg cggccggctc gaggccggtg tgacgatgat cgccatcctg
     5761 atcatcccgc tggcggtgct ggtgcactcg gtgctgagct gggccttcgc ggtcaatagc
     5821 cggcccggct ggggaagtac catcttcgcc ccgtatttcg tcgtcgcggc cctctattcc
     5881 ggcgtgggga tggtcatcct ggtcgtggcc ggctttcgcc gcgggtaccg gctggagcgc
     5941 ttcatcggcg tcaggcactt ccgctacctc gcctacctga tgctggcgct cgacctgctc
     6001 tacctctact tcacgttcac tgagatgttg accgaggggt acacgctcaa tgaggagatc
     6061 acgccgattc tgacggccgt cctggtcggc cagtacgcgc cgttcttctg gctcttcgtc
     6121 gtcggcgccg ggctcatccc gctgctgctg gtggcgttgc ccatgacgcg taccgtcccc
     6181 gggatcgtgg tcgcggccgc gctggtcgtc atcggtatgt gggtcaagcg cctggtgatc
     6241 gtggtgccgg cggcagcgca gccgatgatc gccggcgcct ggggcacctt ccacccgacc
     6301 tgggtggcga tcgcgatcac ggccggtggc gccgccgcgg tgccgcttct cctcatgctg
     6361 ttcttcaagt tcttcccgat cctggcgatc tacgagatcg aagagctggc cgccacgccc
     6421 gcgcccggcc cggccgagct cgccaacgcg caacgagcgg gcgcggagaa aggactggtg
     6481 ggatgagact gcgaagcatg ctggtgctgg cggtgattct tggcgggagc ctcctggtcg
     6541 cgtttcccga cggcggcgcg ctggcagctt ccccgccgcc ggcggccgcg cggctggcgc
     6601 tggcgacgac gccgctgctc gaccacaagg ggcacgtgat cccgggcaag tatctggtcg
     6661 tggcgacgct caccgcggcc gacgggaagc cgctgagcaa ccagtcgatc accttcttcg
     6721 aacagcagga gtttctgggc acgatgcgcc aggtggggct gggaatggcg acgaccgatg
     6781 cgaccggcac cgccgccgtc gcctaccagc ctgctcaggc gggtgcgcac gcgctggcag
     6841 cgcgcttcgt cggcgacgca caggtggcgg cggcagatgc
//
LOCUS       DASUBI010000748         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_960433, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000748 DASUBI010000000
VERSION     DASUBI010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960433"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFU72_14600"
     CDS             complement(<1..1395)
                     /locus_tag="VFU72_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEU5424772.1"
                     /translation="MSQSVAGLVSPEQRFGFQMGADRKLVRWPDMLAYFRDIAAHSDR
                     VHYEELGKATEGQPFVLLTISSAENLARLEELRAIQKRLADPRDLTAEEAETLVEQGR
                     AIVMITCSIHATEVGAVQATPEWVYELATRDDEAARHILDNVVLLLVPSLNPDGMELV
                     ADWYARTLGTPAEGTQPPQLYHTYTGHDNNRDWFMFTQIENQLAVRIQNAWHPQIVFD
                     QHQMGSDGARYILPPFIDPYDPNVDPVLRAEVAQMGTAMATELTAAGKAGVAVNVIFD
                     AFSPSRAYQHYHGGVRILSEAASCKIATPITIPPESLREARGFDPRTARWNHPLPWKG
                     GEWHLRDIVEYNKIAVYACLRNAAAYRRQWVHNFWTVQQRAVERAAPYAFVLPANQRD
                     HGTTAELLQALRTGLVEVEQARAPFTAGGVEYPAGSYVIRLAQPFGAFAKTLLEIQHY
                     PDLRQYPGGPPRPPY"
     gene            1581..1778
                     /locus_tag="VFU72_14605"
     CDS             1581..1778
                     /locus_tag="VFU72_14605"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEU5424773.1"
                     /translation="MEVTYDPKTDTLQIVFRSAPVEESDEEKPGVILDYDRDGNIVGL
                     EILDANTRVDNPREFAYAIVS"
     gene            1924..2379
                     /locus_tag="VFU72_14610"
     CDS             1924..2379
                     /locus_tag="VFU72_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5424774.1"
                     /translation="MATRILGLLLLGGVLLVAAGCGGVQFGAAQQAPSPTAAGAEPGA
                     TETTGQLAVRGQILFNQFHCNTCHTVDGSRAAGPTMKGLYGSTVVLDTGQSVVANDQY
                     LRDSILSPDAQIPKGYAPQVMSLAMGDMMNRVAADDNVSALVAYIESLK"
BASE COUNT          366 a          762 c          874 g          380 t
ORIGIN      
        1 gtagggcggc cggggcggcc cgccgggata ctggcgcagg tcggggtagt gctgaatctc
       61 gagcagcgtc ttggcgaagg cgccgaaggg ctgagccagc cggatgacgt aggagccggc
      121 cgggtactcg acgccaccgg cggtgaaggg cgcgcgcgcc tgctcgacct ccaccagccc
      181 ggtccgcagc gcctggagca gctcggcggt ggtgccgtga tcgcgctggt tggcggggag
      241 gacgaaggca tagggcgccg cgcgctcgac cgcgcgctgc tgcaccgtcc agaagttgtg
      301 gacccactgg cggcggtagg cggcggcgtt gcgcaggcag gcgtagaccg cgatcttgtt
      361 gtactcgacg atgtcgcgca ggtgccactc gccccccttc caggggagcg ggtgattcca
      421 gcgggcggtg cgcggatcga agccgcgcgc ctcgcgcagg ctctccggtg ggatggtgat
      481 cggggtggcg atcttgcagc tggccgcctc ggagaggatg cgcacgccgc cgtggtagtg
      541 ctggtaggcg cgcgagggcg agaaggcgtc gaagatgacg ttgacggcca cgcccgcctt
      601 gccggccgcg gtcagctcgg tggccatggc ggtgcccatc tgggccacct cggcgcgcag
      661 caccggatcg acgttggggt cgtaggggtc gatgaagggc ggcaggatgt agcgcgcgcc
      721 gtcggagccc atctgatgct ggtcgaagac gatctgcggg tgccaggcgt tctggatccg
      781 cacggcgagc tggttctcga tctgggtaaa catgaaccag tcgcggttgt tatcgtggcc
      841 ggtgtaggtg tggtagagct gcggcggctg cgtaccctcg gcgggcgtgc ccagcgtcct
      901 ggcgtaccag tcggccacca gctccatgcc gtcggggttg agcgagggga ccagcagcag
      961 caccacgttg tcgaggatat ggcgcgccgc ctcatcgtcg cgcgtggcca gctcgtagac
     1021 ccattccggc gtggcctgca ccgcgccgac ctcggtcgcg tggatgctgc aggtgatcat
     1081 cacgatggcg cggccctgct cgacgagcgt ctcggcctcc tcggcggtca gatcgcgcgg
     1141 gtcggccagc cgcttctgga tcgcgcgcag ctcctccagc cgcgccaggt tctcggccga
     1201 tgagatggtg agcaggacaa agggctggcc ctcggtggcc ttacccagct cctcatagtg
     1261 cacgcgatcg gagtgggcgg cgatgtcacg gaagtaggcg agcatgtcgg gccagcgcac
     1321 cagcttgcgg tcggccccca tctggaaacc gaagcgctgc tccggcgaaa ccagcccagc
     1381 cacagactga ctcatcggtt cctcctcacg cccccagcga gtgtacgtcc gatcctagcc
     1441 caagaagcgg cggcgcgcta cagccgaacg gcccgaattc gcggcaattg tccgcctgat
     1501 gtcgtgtcgc ggccggcgtg ctagtctgtc gattgtctgg ggcgctcggt gcacctcatc
     1561 ggcaagtact ggagagcgtc gtggaagtca cctacgaccc caagacggac acgctccaga
     1621 tcgtttttcg ctccgcgccg gtggaggaga gcgacgagga gaagccgggc gtgattttgg
     1681 actacgacag ggacggcaac atcgtcgggc tggagatcct tgacgcgaac actcgcgtcg
     1741 acaacccgcg cgagttcgcc tacgccatcg tctcgtgatc ttccgcgctg ctcgcgaccg
     1801 tccggcttct cctgacggca tgcgcccgcg cagggtgtag gcttccagcg agcgcgtgat
     1861 cgagctgggc gagaccgggg acgccgcgcc cgctgcgggg actgctagag agtgtgtgcg
     1921 gtgatggcga cgcggatcct gggattgctg ctgctggggg gtgtgctgct cgtggccgcg
     1981 ggctgcggtg gcgtccagtt cggggcggcc cagcaggcac ccagccctac ggcggccggg
     2041 gccgaaccgg gcgcgacgga gacgaccggc caactggcgg tgcgcggcca gatcctcttc
     2101 aaccagttcc actgcaacac ctgccacacc gtcgacggct cgcgcgccgc cgggccgacc
     2161 atgaaggggc tctacggcag cacggtggtg ctcgacaccg gccaatcggt cgtggccaac
     2221 gaccagtacc tgcgcgacag catcctcagc cccgacgcgc agatccccaa gggctacgca
     2281 ccccaggtga tgtcgctggc catgggcgac atgatgaatc gggtcgcggc cgacgacaac
     2341 gtgagcgcgc tggtggccta tatcgagagt ctgaagtagg gg
//
LOCUS       DASUBI010000749         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_305781, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000749 DASUBI010000000
VERSION     DASUBI010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305781"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..1053)
                     /locus_tag="VFU72_14615"
     CDS             complement(403..1053)
                     /locus_tag="VFU72_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424775.1"
                     /translation="MAGELVSFHGYHGRFRIPILAHLLACAALALTLTVACDGARGPA
                     VGVSGTWVAGAQATAPTTPAALNPFATPAFSPSSSPQASPAASPHASPATPFVATPQP
                     DNRVSATLATGVGPNDQPVDPGDSFPPDTAKLYLVFRTHGLPGQSHLEAIWIADRVDA
                     PVPANYEVTRSTIAVQGDQAGNFSLSKPNPGFPLGQYHVDLYLDGTLIGSFPFTIK"
     gene            complement(1110..2048)
                     /locus_tag="VFU72_14620"
     CDS             complement(1110..2048)
                     /locus_tag="VFU72_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5424776.1"
                     /translation="MARGGIRRGLWRQLARWRSIPPIDLLYRAGRDFARHHGTVYAAA
                     SSYYALISLFPLLILLATVFGWVAHGTGLQDQIVNEIVNQFPPGINLAKQVNSVLKGA
                     TARSGLIGLLSLLATMWTASGLFGVLRRALNTAFDVPEAHSYFHGRLYDMLGVGAVGG
                     LALLSVAATAALSIARAVTSEFSESTLLSVGWTVVFFLVPLGVSYAALFLLYRVIPNQ
                     RVDTRAIRLGALLAAVAFELVKLGFTLYLATLGSYQQVYGALGSVVALLVFVYLESGV
                     VIFAAEVASELAKDRAGTASPAPRRAPPRRIKVPQR"
     gene            2173..3090
                     /locus_tag="VFU72_14625"
     CDS             2173..3090
                     /locus_tag="VFU72_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5424777.1"
                     /translation="MTSQPYARRTTIIKRTLLGALLLNVGIAAAKLGYGHVSNSLAIT
                     ADGLNSLMDAASSLVGIAGLVIAARPPDPNHPYGHRRFETLTSLVIGISMILVLLEIL
                     QRAWQRFEHGGQPEVTLASFVVMFVTLGVNLVITTWERRNARRMHSSILAADAQHTLS
                     DAFVALSVIAGLVAIRLGFPQADILLSLVVAGVIAFAAWSILHHAALSLSDEAAAETP
                     DIEQAARVIAGVEGVHNIRTRGGEGFVWVDLHIQVDPSLRVDRAHEIASEVAARVEQT
                     LGVPSDVTVHVEPANPAHLKPERGYHPGP"
BASE COUNT          600 a         1185 c         1194 g          517 t
ORIGIN      
        1 tccagggcac gccctatgag gtcgagttgg gcctactcgg caatcaggta ctgaccgaac
       61 gcggctggct gcccgcgccg agcccgtgag gcaagcgccc gtcgccgaaa cgcactgtgg
      121 ttgggcaagg caggagagat ccttcaccgc ggttcacgat gacaggaaaa cggccaaccc
      181 tcccccctgt catcccatcg acaagctcag ggcaggctct gaggccgcag ccgaaggatc
      241 ccccactggt gtccctcgcg ccgatgctcc atttcgccgc catgcgtgca cgctcaaccc
      301 gagaattcca ttctggacgg agccgggcat cgcgcggcac aggcgctccg ggtcaacgcc
      361 gaacacgtgg cgtcgctccc gtcacgccgc gatacgccag cgctacttga tcgtgaacgg
      421 aaagctgccg atcagcgtgc cgtcgaggta gaggtcgacg tggtattggc cgagcgggaa
      481 gccggggttc ggcttggaga gactgaagtt ccccgcctga tcgccctgca ccgcgatcgt
      541 cgagcgcgtc acttcgtagt tggccggcac cggcgcgtcg acccggtccg cgatccagat
      601 cgcctccagg tggctctggc ccggcagccc gtgcgtccgg aaaaccaggt agagcttggc
      661 ggtgtccggc gggaagctgt caccgggatc gaccggttga tcattcggcc cgacgcccgt
      721 ggccaacgtg gcgctgacgc ggttgtccgg ctggggagtg gcgacgaagg gcgtggccgg
      781 gctggcgtgt gggctcgcgg cgggtgaagc ctgcgggctg gacgacgggc tgaaggcggg
      841 cgtggcgaag ggattgagcg ccgccggagt cgtcggcgcc gtggcctgcg caccggcaac
      901 ccaggtgccg ctgactccga ccgccgggcc gcgcgcgcca tcgcaggcga ccgtcagcgt
      961 tagcgccagc gcggcgcagg ccagcaggtg ggccaggatg ggtattctga atcggccgtg
     1021 gtaaccatga aacgacacca gctcgcccgc catcatgtgc gcctgcgcca aataaccggc
     1081 ggaagcctat ccagtggtgc gtgccagtgt caacgctgcg gcaccttgat ccggcgcggc
     1141 ggagcgcggc gcggcgcggg cgaggccgtg ccggcgcggt ctttagccag ctcggaggca
     1201 acctccgccg cgaagatcac cacgcccgac tccaggtaga cgaagacgag cagggcgacc
     1261 acgctgccca gtgcgccata gacctgctgg tagctgccga gcgtcgccag gtagagcgtg
     1321 aagcccagct tgaccagttc aaacgccacc gcggccagca gggcgcccag acggatggcg
     1381 cgcgtgtcga cgcgctggtt cgggatgacg cggtagagca ggaacagcgc cgcgtaggag
     1441 acgcccagcg gcaccaggaa gaagacgacc gtccagccga ccgacagcag cgtggactcg
     1501 gagaactcgg aggtcacggc tcgcgcgata ctgagcgcgg ccgtggccgc gaccgagagg
     1561 agcgccagcc cgccgaccgc gcccactccc agcatgtcgt agagacggcc gtgaaagtag
     1621 gagtgcgcct cgggcacgtc gaaggcggtg ttgagcgccc ggcgcagcac gccgaagagg
     1681 ccggaggcgg tccacatggt cgccagcagg ctcaagagac cgatcaagcc gctgcgcgcg
     1741 gtcgcgccct tgagcacgct attgacctgc ttggccagat tgatgccggg ggggaactgg
     1801 ttgacgatct cgttgacgat ctgatcctgc agcccggtgc cgtgcgccac ccagccgaag
     1861 accgtcgcca gcaggatcag cagcgggaag agcgagatca gcgcgtagta ggagctcgcg
     1921 gccgcgtaga cggtgccgtg gtggcgcgcg aaatcccggc cggcacgata gagcaggtcg
     1981 atcggtggga tcgaccgcca gcgggccaac tgccgccaca gaccgcgccg aataccgccg
     2041 cgcgccacct caagccctct cctgcctccc ggcatgtaga atgcagcgtg tcgcagccgc
     2101 gccacttcgc gcaagtatcc acgaccggga tcctcgcgac ccggtccggc gagcgccgat
     2161 cacgaggacg tcatgacgag tcagccgtac gcacggcgca ccactattat caagcgaacg
     2221 ctccttggtg ccctcctcct gaacgtggga attgccgcgg cgaaattggg ctacgggcac
     2281 gtcagcaaca gtctcgccat caccgccgac gggctgaact cgctgatgga cgccgcctcg
     2341 agtctggtcg gcattgccgg cctggtcatc gccgcgcgcc cgcccgaccc caatcaccca
     2401 tatgggcacc ggcgcttcga gacgctcacc tcgctggtga tcggcatctc gatgatcctg
     2461 gtcctcttgg agatcctgca gcgtgcctgg cagcgcttcg agcacggcgg ccagcccgag
     2521 gtcacgctgg ccagcttcgt cgtcatgttc gtcacgctcg gggtgaatct ggtgatcacg
     2581 acctgggaac gccgcaacgc ccgccgcatg cacagctcga ttctggccgc cgacgcccag
     2641 cataccctca gcgacgcctt cgtcgcgctc tcggtcatcg ccgggctggt cgccatccgg
     2701 ctcggctttc cgcaggccga catcctcctc tcgctggtag tggccggggt gatcgcgttc
     2761 gcggcttggt cgatcctcca ccacgcggcg ctctcactct ccgacgaggc cgcggccgag
     2821 acgccggaca tcgagcaggc ggcgcgcgtg atcgccgggg tagagggcgt gcacaatatc
     2881 cgcacgcgcg gcggagaggg attcgtctgg gtcgatctgc acattcaagt cgacccatcg
     2941 ctgcgcgtcg atcgcgcgca cgagatcgcc agcgaggtgg ccgcccgcgt cgagcaaacg
     3001 ctgggcgttc cctccgacgt cacggtccac gtcgaaccgg ccaacccggc ccacctcaag
     3061 cccgagcgcg gctatcaccc aggaccgtga gcgccgcggg cgagcgcctc ctgagcgatc
     3121 aatatcgcag accgcgcgcc gcgccgcggt cgtccttgac aggccggaac cgcccgggta
     3181 tactacccga gcttcaccga gcggacgcgc tccgacggtg agtgcggggc ggcccgccgg
     3241 caggcgggcg tttttctgcc cggctcgctc aaacgccgcg caacccttga cgggggcggc
     3301 gaaagtgagt aaaatagaaa ggttctgtcc cgcgctaagt accgcggaca gacggcacct
     3361 tgacaactga ggcgtagttc gactgggccc ttgggtcgcg gtggggttgg tgacactcgt
     3421 gctggcgatc cggatagcga gcaatcagcc catgaagggc cgccctgtgg ggcgattgat
     3481 ggagagtttg atcctg
//
LOCUS       DASUBI010000750         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1127835, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000750 DASUBI010000000
VERSION     DASUBI010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127835"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFU72_14630"
     CDS             <1..220
                     /locus_tag="VFU72_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424778.1"
                     /translation="KTGWTPSAGECLLFEATHRKKVLIGVILDSAPTNEGVTFVDAAK
                     LLNWAFGLHVPIPPPTPPPLRPKFAVGG"
     gene            251..949
                     /locus_tag="VFU72_14635"
     CDS             251..949
                     /locus_tag="VFU72_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5424779.1"
                     /translation="MTGLATQLAAADGGAGPRVLIVEDDIGIATQLVRGLVRGGYRVD
                     HVTTGRDALARVVPDVVLLDLELPDGDGVTVCRKLRERHHVAIIVITAYGEESDRVTA
                     LDAGADDYLVKPFGLAELQARIRAVLRRVRPGGEIVRHGQLTVDLRTRKVTVDGHDVA
                     LTPKEFDILEVLALDPGRLVSRQEVIEAAWDAHWYGPTKVLDVHVAALRRKLGVPGLI
                     ETAYGRGFRLGDPH"
     gene            949..>2192
                     /locus_tag="VFU72_14640"
     CDS             949..>2192
                     /locus_tag="VFU72_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5424780.1"
                     /translation="MARRITLTVIALVVVILGIVAVPLGLHVASEDRIDFTKQTAAEA
                     ATVASVAEEHLDDQAPGVSLAQVIADVQRRGYQVAVYQPSGQPLTGTSAVAGASSAMI
                     SSALTGSTSYSSPSPDRLLVVTPVVKDESPTAIGAVALSRSTETLDHRIRALWTWLAL
                     ASVVGLLAAALTTTALARWVSSPLSGLESAARRLGAGEFAARSPAGAGPREVRRLAGT
                     FNMMAGRLDALVTGHRAMMADVSHQLRTPLAALRLRLDVLALAAPPPLADELAGAQGE
                     VARLSRMVDGLLAVARAENVTSPPVPVPVDAVIKDRVAAWLPAAEEKHAALEARADGP
                     LTARLGEGHLEQILDNLLANALEAVPIGGRIKVSGRQAGSIVRVTIIDDGPGMSVAER
                     RAALRRFVTATPGGTGLGLAIV"
BASE COUNT          353 a          758 c          751 g          330 t
ORIGIN      
        1 caaaaccggc tggactccat ccgccggcga gtgcctgctg ttcgaggcca cccatcgcaa
       61 gaaggtcctg atcggcgtca tactcgacag cgcgccgacc aatgagggcg tgacgttcgt
      121 cgacgccgcc aagctgctga actgggcgtt cggcctgcat gtgccgatac cgccgccgac
      181 gccgccaccg ctgcgaccta agttcgccgt cggcggctag gacctcgaga gatgtcttaa
      241 cctcggtgag gtgaccggac ttgctaccca gttggccgct gccgatggcg gcgccggccc
      301 gcgggtgctg atcgtcgaag acgacatcgg catcgcgacg cagctagtcc gtggcctggt
      361 caggggcggc taccgggtcg atcacgtgac cacgggtcgc gacgcgctgg cccgtgtcgt
      421 gcccgacgtg gtgctgctcg acctagaact accagacggc gacggcgtca ccgtgtgccg
      481 aaagctacgc gagcgccacc atgtggcgat catcgtgatc actgcttacg gcgaagagtc
      541 agaccgggta accgcgctgg acgccggcgc cgacgactat ctggtcaagc cattcgggct
      601 tgccgaactc caggccagga tccgcgccgt actccggcgg gtcaggcccg gcggagagat
      661 cgtcagacac ggtcagctca cggtcgacct gcgcacaagg aaagtgaccg tcgacggcca
      721 cgatgtggca ctgaccccca aggaattcga catactcgaa gtactcgcac tcgaccccgg
      781 caggctcgtg agccgccagg aggtgattga agccgcctgg gatgcgcact ggtacgggcc
      841 gaccaaggtg ctcgacgtgc acgtcgccgc gctgcgccgc aagctcgggg tacctggcct
      901 gatcgagacg gcctacggac gcggcttccg gctcggcgac ccgcactgat ggcacgcagg
      961 atcacgctga cggtcatcgc cctcgtggtc gtgatacttg gcatcgtcgc ggtgccgctc
     1021 ggcctgcacg tggccagcga ggacaggatc gatttcacca agcagaccgc ggccgaggcg
     1081 gcaaccgtcg cgagcgtcgc cgaagagcat ctggacgacc aggcacccgg cgtttcgctg
     1141 gcccaggtga ttgccgacgt acagcgccgt ggctatcagg tcgccgtata ccagccttcg
     1201 ggccagccac tcaccgggac ctcagcggtg gccggtgcct catcggcaat gatcagctcg
     1261 gcgctaaccg gcagcacttc ctactcaagc ccatcacccg atcggctgct cgtggtcacg
     1321 cccgtggtca aagacgagag cccgacggcc atcggcgccg tggcgctgtc caggtcgaca
     1381 gagacgcttg atcaccggat tcgcgcgctg tggacgtggc tcgcgctggc gtcggtggtc
     1441 ggcctgctag cggcggcgct gaccacgacc gccctagccc gctgggtcag cagcccgctc
     1501 agcggcctgg agtccgcggc caggcgactt ggcgcgggcg agttcgccgc caggtcgcct
     1561 gccggggctg gcccgcgcga ggtgcgcagg ctggcaggca ccttcaacat gatggcgggc
     1621 aggctcgatg cgctggtgac cggccaccgg gcgatgatgg cggacgtgtc acaccagctc
     1681 agaacgccgc tcgcggccct gcggctccgt ctcgacgttc tcgcgcttgc cgccccgccg
     1741 ccgctcgccg atgagctcgc cggcgcccag ggtgaggtcg cgcgcctgtc caggatggtc
     1801 gacgggctgc ttgccgtggc tagggctgag aacgtcacga gccctccggt gcccgtgccg
     1861 gtcgacgcgg taatcaagga ccgggtcgcc gcatggctgc ccgccgccga ggagaagcat
     1921 gccgcgctgg aagcgcgcgc cgacggaccg ctgaccgcca ggctcggcga agggcatctg
     1981 gagcagatac tcgacaacct gctcgccaac gcgcttgagg cggtcccgat cggcggtcgc
     2041 atcaaggtca gcgggcgcca ggctggcagc atcgtccgcg tcacgatcat cgacgacgga
     2101 cccggcatga gtgtcgccga gcgcagggcg gcgctgcgcc gcttcgtcac cgcaacgccc
     2161 ggcggcaccg gccttgggct ggccatcgtg ca
//
LOCUS       DASUBI010000751         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_190782, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000751 DASUBI010000000
VERSION     DASUBI010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190782"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFU72_14645"
     CDS             complement(<1..420)
                     /locus_tag="VFU72_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5424781.1"
                     /translation="MSERAQPVERLRGMVDLSPEALCRREMVREQVLGVLARHGYRQV
                     ETPVVEATDLFLRKSGGERAAQMYAVRYREREIALRPEHTAPILRFYLDALQGEPLPL
                     RLSYGGPVFRYEKPQAGRTRQFTEVGGELLGGSGPAAD"
     gene            631..1053
                     /locus_tag="VFU72_14650"
     CDS             631..1053
                     /locus_tag="VFU72_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5424782.1"
                     /translation="MYEEKSPAAGPEPRSPVRQVHRSRTNRVIAGVCGGLGEYLALDP
                     VLLRVAFVGLALANGIGVLLYIVAWIAMPQAVEGAEETRAPAVTMHQARLFVGGALVA
                     LGAFLLLRISIPWFDARLVWSALLIAVGALILAKGLER"
     gene            1050..1691
                     /gene="liaF"
                     /locus_tag="VFU72_14655"
     CDS             1050..1691
                     /gene="liaF"
                     /locus_tag="VFU72_14655"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HEU5424783.1"
                     /translation="MSTQPLGRSPGEGRAVTASLGTIVVGAILVALGLLWFIDTLDVV
                     TLNYGALLAVGLVVVGVGLVAGARSGVHGGLVVLGLMLTLALTVATAVSVPLHGTVGD
                     QNISLRAIEDVQSKYNVTAGNLTLDFQRVAFPAGDTNVEVRVGAGNLSIDVPRDVAVQ
                     VRYQVGVGDVTVFGHNESGIGMDSQRETAGFASADHRLILDVNVGAGELEVRQ"
     gene            1688..1879
                     /locus_tag="VFU72_14660"
     CDS             1688..1879
                     /locus_tag="VFU72_14660"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU5424784.1"
                     /translation="MSEQTFHPTAAFIGLIFIVLGVVFLLDQLDVIALQARYIVPAMV
                     IGLGLAIMAGSLTRRDRFS"
     gene            complement(1988..>2179)
                     /locus_tag="VFU72_14665"
     CDS             complement(1988..>2179)
                     /locus_tag="VFU72_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424785.1"
                     /translation="ALLAGVRLFDIYRGPAIPEGSKSLAFRVTLEAPDRQLEEREVER
                     VRGRIEAQLKRRVKGALRT"
BASE COUNT          308 a          715 c          769 g          387 t
ORIGIN      
        1 gtcggcggcc ggcccgctgc cgcccagtaa ctcgccgccg acctcggtga actggcgcgt
       61 ccggcccgcc tggggtttct cgtagcggaa gaccggcccg ccgtagctga gccgtaaggg
      121 cagcggctcg ccctgcagcg cgtcgaggta gaagcgcagg atcggggcgg tgtgctcggg
      181 gcgtagcgcg atctcgcgct cgcggtagcg cacggcgtac atctgggccg cgcgctcgcc
      241 gccagatttg cgcaggaaga gatcggtggc ttcgacgacc ggtgtctcga cctggcggta
      301 gccatgccgt gccagtacgc ccagcacctg ctcccgcacc atctcgcggc gacacagcgc
      361 ctcgggggac aggtcgacca tgccccgcag gcgctcgacc ggctgcgcgc gctcgctcac
      421 tccctgccca ctctcccccc tgcgcttcag cgtgccgaca acccggcagt ctatcacgcg
      481 cggagccgcg cggcgtgtca cgaagctgat gcgcgcgcgg gcgccgtttg acgggactgt
      541 aacggtccgg ccggtatgct caatctgccg cggcccacga aggcgtggac aacgggccgc
      601 cggcctagac tctgagggga ggctctgcca atgtatgagg aaaaatcgcc cgcggcgggt
      661 ccggaaccgc ggtcgccagt gcgtcaggtg caccggagcc gcacgaatcg ggtcatcgcc
      721 ggcgtctgtg gtgggcttgg cgagtatctc gctctcgatc ccgtcttgct tcgtgtcgcg
      781 ttcgtgggac tggcgctggc caatggcatc ggcgtgcttc tctacatcgt cgcctggatc
      841 gcgatgccgc aggccgtgga gggcgctgag gaaacgcgtg cgccggccgt gaccatgcac
      901 caggcacgcc tgttcgtggg cggagcactg gtcgcgctgg gcgcgttcct gctgctgcgc
      961 atctcgatcc cctggttcga tgcccggctg gtctggtcag ctctgttgat cgcggtcggt
     1021 gccctgattc tggcgaaggg gttggagcga tgagcacgca accgcttgga agatcgccgg
     1081 gggaaggccg cgccgtcacg gcctcgctcg gcacgatcgt cgtgggcgcc attctggtgg
     1141 cccttggact actctggttc atcgatacgc tcgacgtggt gacgctcaat tacggtgcgt
     1201 tgctggccgt cgggctagta gtggtcgggg tcgggttggt cgccggcgcc cgcagcggcg
     1261 ttcacggcgg cctggtcgtg ctcgggctga tgctgacgct ggcgctgacg gtcgcgaccg
     1321 cggtcagcgt gccgctgcat ggcaccgtcg gcgatcagaa catcagcctg cgggcgatcg
     1381 aggacgtgca gtcgaaatac aacgtgacgg ccggcaacct cacgctcgac tttcagcggg
     1441 tggcgtttcc agccggcgac acgaacgtcg aggtacgggt tggagccggt aatctgtcca
     1501 tcgacgtccc gcgcgacgtc gccgtgcagg ttcgctacca ggtcggcgtc ggtgacgtga
     1561 ccgtcttcgg acataacgaa tccggcattg gcatggacag ccagcgcgag acggccggct
     1621 tcgcgagcgc cgaccaccgg ctgatcctcg acgtcaacgt gggggcggga gaactcgagg
     1681 tgcggcaatg agcgagcaaa cgttccatcc gacggcggcc ttcatcggac tgatcttcat
     1741 cgtgctgggc gttgtgttcc tgctggatca gctcgatgtc atcgcactgc aagcgcgcta
     1801 catcgtgcct gccatggtga tcggtctggg attggcgatc atggccggca gcctgacacg
     1861 gcgcgatcgg ttctcctgat gagatcgtct ctcgcggggt aatgtgggag tgctgcggcg
     1921 aacgacgcct cgggcaatcc agcccggggc gtcgtttctt ctcgcgcgtg cgccgcgcgc
     1981 gtggcaccta cgtgcgcagc gcccccttga cgcgtcgctt gagctgcgcc tcgatgcggc
     2041 cacgcacgcg ctcgacctca cgctcctcaa gctggcggtc gggcgcctcc agggtgacgc
     2101 ggaaggccag gctcttgctc ccctccggga tcgcggggcc gcggtagata tcgaagaggc
     2161 gcacgccggc caacagcgc
//
LOCUS       DASUBI010000752         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_251496, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000752 DASUBI010000000
VERSION     DASUBI010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251496"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFU72_14670"
     CDS             complement(<1..648)
                     /locus_tag="VFU72_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5424786.1"
                     /translation="MNDVAVMILAGGQGERLSILSRQRAKPAVPFAGKYRIIDFALSN
                     CVNSGLFDVAVLTQYRPHSLNEHIGHGRPWDLDRERNGGVMILQPYLGRAPSGWYRGT
                     ADAIYHNLFFVQRGQFDDVLILSGDHIYAMDYAPMIAKHRELNADVTIAVQPVPWEDA
                     SRFGLVEVDEHDHVIDFDEKPEQPRSNLASMGIYVFKRDVLLNLFDETSGDMTDFG"
     gene            788..1678
                     /gene="accD"
                     /locus_tag="VFU72_14675"
     CDS             788..1678
                     /gene="accD"
                     /locus_tag="VFU72_14675"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5424787.1"
                     /translation="MRELFRRQPKFTTGNEDQTSIPDDLWIKCANCGELIYSREAERL
                     LQVCPRCGHHRRLSARQRIAALVDDGSFCELDAGLLPADPLQFVANGDAYAAKVVATR
                     RKTGTAEALVCGSAAICGRRVTLAVTEFGFLGASMGSVFGEKLVRAVEHALAERLPLI
                     TISSSGGARMHESMFSLMQMAKTTAALARLGEARLPHIALLTDPCYGGVTASYATVAD
                     VIVAEPGAMIGFAGPRVIEQITRQKLPEGFQTAEFLLEHGMIDLVVPRRELPQRLSLL
                     LGHFAAAPVVEPILAGEPRV"
     gene            1671..2522
                     /locus_tag="VFU72_14680"
     CDS             1671..2522
                     /locus_tag="VFU72_14680"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5424788.1"
                     /translation="MSEPLTAWERVQLARHPQRPHTLDYLHELLEGFVELHGDRIYGD
                     DRALIAGLGTFRGRKVLVLGHQKGANTRENLERNFGMPHPEGYRKALRLIRLAEKFGL
                     PILSFIDTPAADPGLGSEERGQAIAIAENLVALASVRVPTVATVIGEGGSGGALAIGV
                     ADRILMLENAIYAVASPEACATILWKNAAEAPTAAEALRITAADLLQFQIADEVVPEP
                     VPAHEQPVETICRVGDAIARQLSELDALATRGGPEALLACRYQKYRRIGRWSEEHDPG
                     NLLASGH"
BASE COUNT          432 a          878 c          927 g          458 t
ORIGIN      
        1 gccgaagtcg gtcatgtcgc cggacgtttc gtcaaaaagg ttgagcagca catcgcgctt
       61 gaagacatag atgcccatcg acgcgagatt gctgcgcggc tgctccggtt tttcgtcgaa
      121 atcaattacg tgatcgtgtt cgtcgacctc gaccaacccg aaccggctgg cgtcttccca
      181 ggggacgggc tgaacagcga tggtcacgtc ggcattcaat tcgcgatgct tggcgatcat
      241 tggcgcgtaa tccatggcgt agatgtgatc gccggaaagg atcaggacgt catcgaattg
      301 gccgcgttgg acgaaaaaga ggttgtggta gatggcgtcg gcggtgccgc gataccagcc
      361 ggagggcgcg cggccgaggt agggctgcag gatcatgacg ccgccgttgc gctcgcggtc
      421 gagatcccag ggccgcccgt ggccgatgtg ctcgttgagc gagtggggcc ggtactgagt
      481 cagcacggcg acatcgaaga gccccgagtt gacgcagttg ctcagggcga agtcgatgat
      541 gcggtacttg ccggcgaacg gcaccgccgg cttggcccgc tggcgcgaga gaatactcaa
      601 ccgctccccc tgaccaccgg ccaggatcat caccgcgacg tcgttcatct cgtccgctcc
      661 cttcgggagt cgccagcgcc gccgactggt tgccgctcgc ccgctcaaag ctcgggtcat
      721 gctccctgta tgctatactg gcgcgctgca aacggcacgc ggttgcttca ccgacggttg
      781 ggggaccatg cgggagcttt ttcgccgtca accgaaattc accacgggca acgaagacca
      841 aacgtcgatt cccgacgatc tctggatcaa gtgcgccaac tgcggcgagc tgatctatag
      901 ccgcgaggcc gagcggctcc tgcaggtctg cccgcgctgc gggcatcacc gccggttgag
      961 cgcccggcag cggatcgcgg cgctggtcga tgacggctcg ttttgcgagt tggatgccgg
     1021 gttgctgcca gccgatccgc tgcagttcgt cgccaacggc gacgcctacg cggccaaggt
     1081 cgtcgccacc cggcgcaaga ccgggaccgc cgaggcgctg gtctgcggct cggccgcgat
     1141 ctgtggccgg cgggtcacgc tcgcggtcac cgagttcggc ttcctcggcg ccagcatggg
     1201 ctcggtcttc ggcgagaagc tggtgcgggc ggtcgagcac gcgctggccg agcgcctgcc
     1261 cctgatcacg atctcttcct cgggtggggc gcgcatgcac gagagtatgt tctcactgat
     1321 gcagatggcc aagacgacgg cggcgctggc ccgcctcggc gaggcacggc tgccgcacat
     1381 cgcgctgctg actgatccct gctacggggg tgtaaccgcc agctacgcca ctgtggccga
     1441 cgtgatcgtg gccgagccgg gtgcgatgat cggcttcgcc gggccgcggg tgatcgagca
     1501 gatcacgcgg cagaagctgc ccgagggctt tcagacggcc gagttcctgc tggagcacgg
     1561 catgatcgat ctggtggtgc cccggcgtga gctgccgcag cggctgtcgc tgctgctggg
     1621 ccacttcgcc gcggcgccgg ttgtcgagcc catcctcgcc ggagagcctc gtgtctgagc
     1681 cgttgacggc ctgggagcgc gtgcaactgg cgcggcatcc gcagcgcccg cacacgctcg
     1741 actacctgca cgagctgctt gagggcttcg tcgagctaca cggcgaccgg atctacggcg
     1801 acgatcgcgc gctgatcgcc gggctgggca cctttcgcgg ccgcaaggtg ctggtgctcg
     1861 ggcatcagaa aggcgccaac acgcgcgaga atctggagcg caacttcggc atgccgcatc
     1921 cggagggcta ccggaaggcg ctgcggctga tccgcctggc cgagaaattc ggcctgccga
     1981 tcctgagctt catcgatacc ccggcggcgg acccggggct cggctctgag gagcgcgggc
     2041 aggcgatcgc catcgccgag aacctggtcg ccctggcctc ggtgcgcgtg ccgaccgtgg
     2101 ccaccgtgat cggcgaaggg ggcagcggcg gggcgctggc gatcggtgtg gccgaccgca
     2161 tcctcatgct ggagaacgcg atctacgcgg tcgcctcgcc ggaggcttgt gccacgattc
     2221 tctggaagaa cgcggccgag gcgccgaccg ccgccgaggc gctgcgcatc accgccgccg
     2281 acctgctcca gttccagatc gcggacgagg tcgtgccgga gccggtcccg gcgcacgagc
     2341 agccggtcga gactatctgc cgtgtgggcg atgccatcgc gcgccagctc agcgagctgg
     2401 atgcgctggc cacccggggt ggacccgagg cgttgctggc ctgtcgctac cagaaatacc
     2461 gccgcatcgg gcgctggtcc gaggagcacg atccgggcaa cctgctggcg tcaggccact
     2521 agcgcatcgg ggtcgccgcc ctccctcttg atcaatagca ttctccgtgg tattaatcgc
     2581 gcatcattga cggtggggca acgctcgctc tgcaattgcc gggcaacggg aagggccatg
     2641 ccgacaccat tgacctacac gccgccgtgt ccatgaccaa tcaggtcgga catca
//
LOCUS       DASUBI010000753         8507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1206616, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000753 DASUBI010000000
VERSION     DASUBI010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206616"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="cysS"
                     /locus_tag="VFU72_14685"
     CDS             complement(<1..1070)
                     /gene="cysS"
                     /locus_tag="VFU72_14685"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5424789.1"
                     /translation="MSISVTNTLSGRKEPLETIEPGKVRMYVCGPTVYAQAHIGHAMS
                     ATVFDVIKRYLEYRGYEVVHAQNFTDVDDKIIQRAHELGIPPERLAEQLIHEWLDETA
                     ALNIQPATVYPRATHEIDTIITMVAELIASGHAYPVEGGDVFYRVESFPGYGKLGHRT
                     LDEMQAGARIEIDERKENPMDFAVWKAAKPGEPAWESPWGLGRPGWHIECSAMVYDHL
                     GGQVDIHGGGSDLIFPHHENEIAQSEAFCNCEPFARYWLHNGLLQLGGEKMSKSLGNL
                     VTIRQLIEEGNAQSFRLLVLGSHYRSPLTFNAETLEAARRGLARLSGAVRDFNPDQVP
                     LSAPEHPLAAAAAEPRARFEQA"
     gene            complement(1296..1589)
                     /locus_tag="VFU72_14690"
     CDS             complement(1296..1589)
                     /locus_tag="VFU72_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU5424790.1"
                     /translation="MPIYEYACKSCGHRFELKQSFHDEPVAVCPECGEHVQRVLHPAG
                     IIFKGSGWYVTDSRKAGSNGGSETKSETKSDGKPEKAEPAASTTSSTPSKSDK"
     gene            complement(1785..2597)
                     /locus_tag="VFU72_14695"
     CDS             complement(1785..2597)
                     /locus_tag="VFU72_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041359162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU5424791.1"
                     /translation="MRPPTSLSDISDEEWAFVAPYLTLMTPHAPKRRYALREVFNSVR
                     SLARAGVAWRLLPHDFPRWDAVYQQTQRWLAAGCFQAITDDLRLLLRLVAGRSPQPSA
                     AIVDSQTLQSTPESGIRAVSDGHKRKIGSKLHLAVETLGHLLALHVTPANAQDRAQVG
                     VLVAAVHDATGEPVELVSVDQGYTGELPAEVAAEQAIALHVVNLPGAKHGFVLLPRRW
                     MAERAFAWKTRFRRLVRDYERLQATVEGMHLVAFSTLMLQQAVALLSEPPSA"
     gene            complement(2883..5627)
                     /gene="polA"
                     /locus_tag="VFU72_14700"
     CDS             complement(2883..5627)
                     /gene="polA"
                     /locus_tag="VFU72_14700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5424792.1"
                     /translation="MKSAEDPRGQTIMLVDGHGLAYRAFHAVPETLATAKGEVTNAVF
                     GFTSMLLDALRQHQPDYVIVSFDVGRTFRHEAFADYKGTRAPMPGELRHQMKRIHEVL
                     AALNIPVYTREGYEADDVIGTLARMAEAQGMQPLIVTGDSDLLQLVDNAAVVVLPGRQ
                     RFGEYRTYDRPAVEQRYGFAPERLPDYKALVGDTSDNIPGVPGIGDKTAKGLIAKYPS
                     LEAMREQLDEITPPRARQALADNFEQALEGRRLATIVRDVPLEVDIDGCRLRDFDRDH
                     VLDVFRELEFRTLVSRLPEPEAPAKRKLQPVEAAENFTVTTSEQLDAMVAELRAGPAY
                     ALDVETDSLDSLSAALVGISLASSPARAYYIPLRHQPADGQLPLETVRERLSPLFADG
                     PAVYAHNGKFDAEAIEHAGFPRPEITFDSMIAAYLLGETALGLKELAFTRLGLEMQPI
                     TELIGKGKSQITFDQVPIEAATTYGCADVEATYRLVNLLAPELAAQHQTDLFHTIEMP
                     LIDVLIDMEQAGIAIDSDFLITLSAQLSSQISELEREIHQLAGHEFNINSTRQLGTVL
                     FDELNLPTGRRTKTGYSVGQDVLESLADAHPIIAAIGEYRSLLKLRSTYVDALPEQVS
                     PIDGRVHTSFNQTIAATGRLSSTDPNLQNIPVRTEIGRQVRRAFIADNRPGYRPWTED
                     AVFLSIDYSQIELRLMAHMSEDPTLINSFQQGHDIHAATASDVFGVPLDAVTPDMRRI
                     AKTVNFGIMYGMQAFGLSRDTGMPRAEAQRFIERYMARLPGVRRFLDETKLHALEKGY
                     VESIFGRRRYVPDIASRNPNLRLAAERMAINMPLQGTAADIMKLARIRVREALREQQF
                     RARMLLQVHDELLFEAPVSEVPALTEVVREIMENVVELSVPLVAEAAAGPNWDQLEPV
                     "
     gene            5925..6908
                     /locus_tag="VFU72_14705"
     CDS             5925..6908
                     /locus_tag="VFU72_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5424793.1"
                     /translation="MEPVQALAERIVENVERVIVGKRPEAQLVLTALLCRGHVLIEDV
                     PGVGKTVLAKAIARSIGSTFKRIQFTPDLLPSDVTGVSVFNQATMLFEYRPGPVVAQI
                     VLADEINRATPKTQSALLEAMEEAQITVDGTTYALPVPFIVLATENPIEYEGTFPLPE
                     AELDRFLVRLSLGYPGHRGEVEMLNRQHFAHPLESLEQVVSVEELVEAQGQVREVHVD
                     DSLKEYIVSIVERTREHDDVYLGAGPRGSLALYNTARAWAAMSGRDYVIPDDIKALAE
                     PALAHRVIVSPAARMKNVDGRMVIQEVLSVTSVPGSRPSVASPERRAGLRP"
     gene            6918..8186
                     /locus_tag="VFU72_14710"
     CDS             6918..8186
                     /locus_tag="VFU72_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5424794.1"
                     /translation="MNGIKLGVVLVVVLVIAEINHWTTLQKVFVALAALFVVALVYSR
                     LSLRGVALSRSTRADRAQVGQTVTERIRVENRSRVAKLWLEIVDHSDLPGHQMSRVIH
                     LGPNDANRWRVETWCSRRGRFRLGPLTLRSGDPFDLFPTAQEIPHSQELLIYPATVDL
                     SGFRLPVGILPGGNSIQRRTPFVTPNASGVRQYLPGDSFNRIAWTATARTGHLMVKEF
                     ELDPTSDVWIVLDLEAAHHVEAGRPGVSLPLGSQDIPLSFWLDSTEEYAVTIAASLAR
                     HFLDQNRTVGLLTNGQQPIVLPTDRGARQMVKVLELLAVAAADGTVPIAETLMAEGAL
                     FSRNSTVVVISPSTDERWVQAVMELIARHARVVAVVVEPSTFGASESSLMVVSDLAAV
                     GVPTYLVKYGDDISRALTTQGHAMPIRRAS"
     gene            8202..>8507
                     /locus_tag="VFU72_14715"
     CDS             8202..>8507
                     /locus_tag="VFU72_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424795.1"
                     /translation="MRRLAIRDGWSTFILISLVVLSATWSIESADWADGLGMLTTIAI
                     LGLAVGLGVSLARRIPALLAHLLALALGWVVVLFEVTNFLSDAIGGREAKLHFLWHRW
                     "
BASE COUNT         1321 a         2750 c         2988 g         1448 t
ORIGIN      
        1 atcgcctgct cgaagcgcgc gcgcggctcg gcggccgccg cggccaacgg atgctccggc
       61 gcgctgagcg gcacctgatc gggattgaaa tcgcgcaccg cgccgctcag ccgcgccagc
      121 ccgcgccggg cagcctccag cgtctcggcg ttgaaggtca gtgggctgcg gtagtgggag
      181 ccgagcacca ggaggcggaa agactgcgcg ttgccctcct cgatcaactg gcgaatcgtc
      241 accagattgc ccagcgactt gctcatcttc tcgccgccga gctggagcag gccgttgtgc
      301 agccagtagc gcgcgaacgg ctcgcaattg cagaaggcct cgctttgcgc gatctcgttc
      361 tcgtggtgcg gaaagatcag gtccgacccg ccgccgtgga tgtcgacctg cccgcccagg
      421 tggtcgtaga ccatggcgct gcactcgatg tgccagccgg ggcggcccag cccccagggc
      481 gactcccagg ccggttcacc cggcttggcc gccttccaaa cggcgaagtc catcgggttc
      541 tccttgcgct cgtcgatctc gatgcgggcg ccggcctgca tctcgtcgag cgttcggtgc
      601 ccgagcttgc cgtagccggg gaaggactcg acgcggtaga agacgtcgcc cccctcgacc
      661 ggataggcgt gcccggaggc gatcagctcc gccaccatgg tgatgatcgt gtcgatctcg
      721 tgggtggcgc gcgggtagac ggtggctggc tggatgttga gtgccgccgt ctcgtccagc
      781 cattcgtgga tcaactgctc ggccaaccgc tccggcggga tacccaactc gtgggcgcgc
      841 tggatgatct tgtcgtcgac gtcggtgaag ttctgggcgt ggacgacctc gtagccgcgg
      901 tactccaggt agcgcttgat gacgtcgaag acggtggccg acatggcgtg gccgatgtgc
      961 gcctgggcgt agacggtcgg gccgcagacg tacatgcgca ccttccccgg ctcgatggtc
     1021 tccaaaggct ccttgcgccc ggacagggtg ttggtgacag agatcgacat ggcattctcc
     1081 actcagatgt tgcggtcggg tagggggagc agagagcgcc ggggtatgac tccgtgtcta
     1141 ctggctgtga tcagacccgc gactccgctc ctgtcagcga tggcagcggg gacgccgaaa
     1201 cgccggcatc ccctagagac aggaacagag aacgccgtgg ctcggagcgt gtagggtgaa
     1261 gtgagcgggc gctgaggaca ccacgatggc acccgctact tgtcgctctt gctgggcgtg
     1321 ctgctggttg tggacgcggc cggctcggcc ttctccggct tgccgtcgga tttggtctcg
     1381 gacttggtct cggaaccgcc gttgctgccc gccttgcggc tgtcggtgac gtaccagccc
     1441 gagcccttga agatgatgcc ggcggggtgc agcacgcgct gcacgtgctc gccgcactcg
     1501 gggcagaccg cgaccggctc gtcgtggaac gactgcttga gttcgaaacg atgcccgcag
     1561 ctcttgcagg cgtactcgta gatgggcatc tggcccacac ctcccaaagg gtctcgcgtg
     1621 acccgacact tatccatttt agcgcaaagc gcggtccgag aaaacccgat gccgcggcaa
     1681 atcgcgagca acctgcccgg ccaacgtgtg tcaatcgcaa gcagcgctga cagtcgtgct
     1741 cctagtacac gacactgacc cacgcgctcg gtcaccagag cctgttatgc acttggcggc
     1801 tcactaagga gggcaacggc ttgctggagc atcagcgtac tgaaggccac cagatgcatg
     1861 ccctccacgg tcgcctgcaa ccgttcgtag tcgcggacca agcggcggaa gcgcgtcttc
     1921 caggcaaagg cgcgctcggc catccagcgg cggggcagca agacgaagcc gtgcttggcc
     1981 cccggcaggt tgaccacgtg gagcgcgatg gcctgttccg ctgcgacttc cgccggtaac
     2041 tcacccgtgt agccttgatc gacggagacc agttccacgg gctcaccggt cgcgtcgtgg
     2101 accgctgcga ccaagacccc aacctgggcc cggtcttgtg cgttggccgg ggtcacgtgc
     2161 agcgccagga gatggcccag cgtctcgacc gccaggtgga gcttggagcc tatcttccgc
     2221 ttatgcccat cggagacggc tcggatccca ctctcgggcg tgctctgcag agtctgactg
     2281 tcgacgatgg cggccgaggg ctgggggctg cggccggcca ccagccgcaa gaggagccgc
     2341 aaatcgtcgg tgatggcctg gaagcagccg gcggccagcc agcgctgggt ctgctggtag
     2401 acggcgtccc agcggggaaa gtcgtgcggc agcagccgcc aggcaacgcc cgctcgggcc
     2461 agcgaacgta cggaattgaa gacctcgcgc agggcgtagc ggcgcttggg ggcgtgcggg
     2521 gtcatcagcg tcaagtacgg cgccacaaac gcccattcct cgtcggagat gtcactcagg
     2581 gaggttggtg gccggattgc tcagtttaca taatcctaag tgtgctttgc gcagtctcac
     2641 gtgactcaca ggctgtagag cggttcgcac ggtcgttggc cgcaatgctt cgtccccgta
     2701 gaggcagatc tccatggctg cgctgcccac ctctcaatgc ggaaagggga agtgaggcaa
     2761 catggcggcg ttgtcgatcg tggccaatag ggcaaccagg gcagacacgg aggtctgctc
     2821 ctaccggagg aggagacccg cgcagacccg ggactacgca aatggtgtca gagcagcgag
     2881 cgctagaccg gttcgagctg gtcccagttg gggccggcgg cggcttcggc gacgagcggg
     2941 acactcagct cgacgacgtt ctccatgatc tcgcgcacga cctcggtcag cgccggcacc
     3001 tcggagacgg gcgcctcgaa gaggagctcg tcgtgcacct ggagcagcat acgcgcgcgg
     3061 aattgctgct cgcgcagcgc ctcgcgcacg cggatcctgg ccagcttcat gatgtcggcg
     3121 gcggttccct gcagcggcat gttgatcgcc atgcgctcgg cggccagccg gaggttggga
     3181 ttgcgcgagg cgatgtcggg cacgtagcgg cggcggccga agatgctctc gacgtagccc
     3241 ttctccagcg cgtggagctt ggtctcgtcc aggaagcggc gcacgccggg gaggcgcgcc
     3301 atgtagcgct cgatgaagcg ctgcgcctcg gcgcgcggca tgccggtgtc gcgcgagaga
     3361 ccgaaggcct gcatgccgta catgatgccg aagttgaccg tcttggctat ccggcgcatg
     3421 tcgggcgtga ccgcgtccag cggcacgccg aagacgtcgg aggcggtggc ggcgtggatg
     3481 tcgtgcccct gctggaacga gttgatcagg gtcgggtcct cgctcatgtg ggccatcagg
     3541 cgcagctcga tctggctgta gtcgatcgag aggaagacgg cgtcctcggt ccaggggcgg
     3601 tagccggggc ggttgtcggc gatgaaggcg cggcgcacct ggcgcccaat ctcggtgcgc
     3661 accgggatgt tttgcaggtt cggatcggtt gagctgaggc ggcccgtggc ggcgatggtc
     3721 tgattgaacg aggtgtgtac ccggccatcg atcggactga cctgctcggg cagcgcgtcc
     3781 acataggtgg accgcagctt gagcagcgag cggtactccc caatggccgc gatgatcggg
     3841 tgggcgtcgg ccaggctctc cagcacgtcc tgcccgaccg agtacccggt cttggtgcgg
     3901 cggccggttg gtaggttcag ctcatcgaag aggacggtgc cgagctggcg cgtcgagttg
     3961 atgttgaact cgtgcccggc gagctggtga atctcgcgct caagctcgga gatctggctg
     4021 gagagctggg ctgacagcgt gatcaggaaa tcgctgtcga tggcgatgcc ggcctgctcc
     4081 atgtcgatca gcacgtcgat cagcggcatc tcgatggtgt ggaacaggtc ggtctggtgc
     4141 tgcgcggcca attcgggcgc cagcagattg accaggcgat aggtcgcctc gacgtcggcg
     4201 cagccgtacg tggtcgccgc ctcgatcggc acctggtcga aggtgatctg cgacttgccc
     4261 ttgccgatca gctcagtgat cggctgcatc tccagcccga gccgggtgaa ggccagctcc
     4321 ttgaggccga gcgcggtctc gcccagcagg taggcggcga tcatcgagtc gaaggtgatc
     4381 tccgggcggg ggaagccggc gtgctcgatc gcctcggcgt cgaacttgcc gttgtgggca
     4441 tagaccgccg gaccatcggc gaagaggggc gagaggcgct cgcgcaccgt ctccagcggc
     4501 agttggccgt cggccggctg atggcgcagc gggatgtagt aggcgcgcgc cgggctgctg
     4561 gccagcgaga tgccgaccag cgcggcgctg agcgagtcga gcgagtcggt ctcgacgtcc
     4621 agcgcgtagg ccgggccggc acgcagctcc gccaccatgg cgtcgagctg ctcggaggtg
     4681 gtgacggtga aattctcggc ggcctcgacc ggctggagct tgcgcttggc gggcgcctcc
     4741 ggctccggca ggcggctgac cagggtgcga aactccagct cgcggaagac gtcgagcacg
     4801 tggtcacggt cgaagtcgcg caggcggcag ccgtcgatgt cgacttccag cgggacatcg
     4861 cgcacgatgg tcgccaggcg gcggccctcc agcgcctgct cgaagttgtc ggccagcgcc
     4921 tggcgggcgc gcggcggggt gatctcgtcc aactgctcgc gcatggcctc cagcgagggg
     4981 tacttcgcga tcagtccctt ggcggtcttg tcgccgatgc cgggcacgcc ggggatgttg
     5041 tcggaggtat cgccgaccag cgccttgtag tcgggcagcc gctccggcgc gaagccgtag
     5101 cgctgctcga cggccgggcg gtcgtaggtg cggtattcgc cgaagcgctg gcgaccgggc
     5161 aagacgacca cggcggcgtt atcgaccaac tgcagcaggt cgctgtcgcc ggtgacgatc
     5221 agcggctgca tgccctgcgc ttcggccatg cgcgccaatg tgccgatgac gtcgtcggcc
     5281 tcgtagccct cccgggtgta gaccgggatg ttgagcgcgg ccagcacctc gtggatgcgc
     5341 ttcatctggt ggcgcagctc gccgggcatc ggcgcgcgcg tgcccttgta gtcggcgaaa
     5401 gcctcgtggc ggaacgtccg cccgacgtcg aagctgacga tgacgtagtc cggctggtgc
     5461 tggcgtaggg catcgagcag catgctggtg aagccgaaga cggcgttggt gacctcgcct
     5521 ttggcggtcg ccagcgtctc gggcaccgcg tggaaggcac ggtaggccag gccgtggccg
     5581 tcgaccagca tgattgtctg gccgcgtggg tcttctgctg acttcaccag gctgctcctc
     5641 tctccatttc tcattatacc gtccggacgc cctctggccc ggggcgcgag cgcgagcgtg
     5701 cgcgtctcgc gcgagggggt acggaaggga cggatttgct tgatataatg gtgacaccag
     5761 tgcagcgtag ggagggtctg cacggcgcct ttcagcggat cgagccggcg cgggccgcgc
     5821 gccagccggc gtcgtccccc acgcaacacc cgaccggccg gcatccgagc cggttgcccg
     5881 gccgtattca acgcaactgc cgaacactgc gggaaggaag acgcatggag cccgttcagg
     5941 cactcgcgga gcgcattgtc gagaacgtcg agcgtgtcat cgtcggcaag cgccccgagg
     6001 cgcagttggt gctgaccgcg ctactctgcc gggggcacgt gctgatcgag gacgtgccgg
     6061 gcgtcggcaa gacggtgctg gccaaggcca tcgcccgcag catcgggtcg accttcaagc
     6121 gcatccagtt cacccccgac cttctcccca gcgatgtgac cggcgtcagc gtcttcaacc
     6181 aggcgacgat gctcttcgag taccgccccg gcccggtcgt cgcccagatc gtgctggccg
     6241 acgagatcaa ccgcgccacc cccaagacgc agtcggcact gctggaggcg atggaggagg
     6301 cgcagatcac ggtcgacggc acgacctacg ccctgccggt ccccttcatc gtactggcga
     6361 ccgagaaccc gatcgagtac gagggcacct tcccgctgcc ggaggccgag ctggaccgct
     6421 tcctggtgcg gctctcgctt ggctatcccg ggcaccgggg cgaggtcgag atgctcaacc
     6481 gccagcactt cgcccatccg ctggagtcac tggagcaggt ggtcagcgtc gaggagctgg
     6541 tcgaggcgca gggtcaggtg cgcgaggtgc acgtcgacga ctcgctcaag gagtacattg
     6601 tctcgatcgt tgagcgcacc cgcgagcacg acgatgtcta cctcggcgcc ggtccgcgcg
     6661 ggtcgctggc gctgtacaac accgcccgcg cctgggcggc catgtccgga cgcgactatg
     6721 tcatccccga cgacatcaag gcgctggccg agcccgcgct ggcccaccgg gtgatcgtca
     6781 gtccggcagc gcgcatgaaa aacgtcgatg gacgcatggt gattcaggag gtgctctcgg
     6841 tcacgtcggt gccgggcagc cgcccctcgg tggcgagccc ggagcggcgc gccggactgc
     6901 gcccctaggg ccccggtatg aacggcatca agctcggggt ggttctggtc gtcgtcctgg
     6961 tcatcgccga gatcaaccac tggacgacgc tgcagaaggt cttcgtcgcg ctggccgcgc
     7021 tctttgtggt cgcgctggtt tacagccgcc tcagcctgcg tggcgtggcg ctgtcgcgca
     7081 gcacgcgcgc cgaccgggcg caggtcgggc agaccgtgac cgagcgcatc cgagttgaga
     7141 accgcagccg cgtcgccaag ctctggctgg agatcgtcga ccattccgac ctgccggggc
     7201 accagatgag ccgggtgatc cacctggggc cgaacgacgc caaccgctgg cgcgtcgaga
     7261 cctggtgctc gcgccgcggg cgcttccgtc tcggcccgct caccttgcgc tccggcgatc
     7321 ccttcgatct ctttcccacc gcgcaggaga ttccgcactc ccaggaactg ttgatctacc
     7381 cggcgaccgt cgatctctcg ggctttcgac tgccggtcgg catcctgccg gggggcaact
     7441 cgatccagcg gcgcaccccg ttcgtgacgc cgaacgcgtc cggggtgcgc cagtacctgc
     7501 cgggcgacag cttcaatcgc atcgcctgga cggccaccgc gcgcaccggg catctgatgg
     7561 ttaaggagtt cgagctggac ccaacctcgg acgtctggat cgtgctcgat ctggaggcgg
     7621 cccaccacgt cgaggcgggg cgtccgggcg taagcctgcc gctcggcagc caggacatcc
     7681 cgctttcgtt ctggctcgat tcgaccgaag agtacgcggt gacgatcgcc gcctcgctgg
     7741 cgcgccactt cctcgaccag aaccgcacgg tggggcttct gaccaacggc cagcagccga
     7801 tcgtgctgcc gaccgaccgt ggcgcgcgtc agatggtgaa agtgctggag ctcctggcgg
     7861 tggccgcggc cgacggcacg gtcccgatcg ccgagacgct gatggccgag ggcgcgctct
     7921 tctcgcgcaa cagcacggtg gtggtcatct cgccctcgac cgacgaacgc tgggtgcagg
     7981 cggtcatgga gctgatcgcg cgccacgccc gcgtggtagc ggtggtggtc gagccgagca
     8041 cctttggagc gagcgaaagt agcctgatgg tggtgagcga tctggccgcg gtgggcgtgc
     8101 cgacctatct cgtgaagtac ggcgacgaca tcagccgagc cctgacgact caggggcacg
     8161 cgatgccgat ccggcgtgcc tcctagcgac ggacgagtca catgagacgc ctggcgatcc
     8221 gagacggctg gtctaccttt attttgatct ccctggtggt gctcagcgcg acctggtcga
     8281 tcgagtccgc cgactgggcc gacggcctcg gcatgctcac cacgatcgcc atcctcgggc
     8341 tggccgtggg cctgggcgtc tccctggcgc ggcgcatccc ggccctgctg gcacacctgc
     8401 tggcgctggc gctcggctgg gtggtcgtgc tcttcgaggt gacgaacttc ctcagcgatg
     8461 ccatcggcgg gcgcgaggcc aagctgcatt tcctctggca ccgctgg
//
LOCUS       DASUBI010000754         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_537883, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000754 DASUBI010000000
VERSION     DASUBI010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537883"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFU72_14720"
     CDS             <1..387
                     /locus_tag="VFU72_14720"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEU5424796.1"
                     /translation="TSDLSRRDKILRDKPANLGDRCYLNPDDPSEVTPGTCTDALPLH
                     ADPRRVAGAPFVDNVVKCTLKPVDFGDYRASFDAAQQARLRAIFPDGVCDYSQPGVGQ
                     VPPAGPWISYGPAPGLTLSGHAASAP"
     gene            551..1771
                     /locus_tag="VFU72_14725"
     CDS             551..1771
                     /locus_tag="VFU72_14725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424797.1"
                     /translation="MSAFTRRERLIVLALAVAVFTGTLNVYLLTPFLKLIAADFGVSE
                     AAAGQLSTTYALAAGLVGLIVAPLVDRYQRRRLLYLAVAIISVSTVFTAVAPSFPALL
                     LAHGLAGFGAAFGASSCFAIASDAFPDQHKRNRSLGLVIAAGGVSSVIGVPILTQIAA
                     ASSWRWAVAALLVPCVVATLLIRVLPSRSLGSRTTFVRDYLASYRRVLHNHETRWLYL
                     ATLTLATAETAPLIYSASIWQGLFDLSLESFGWIFLLGGVCYAIGSNLAPVLLRRRSP
                     REACMLTGGLQFLAALSFPLVDSSLPAALFVYAGPVVMLSALNVVALNVLLQDSLPAD
                     RGAVLSLDGTIAKCGTACAAIFGGIILAAAGEGAIIPLINLVLPFTLLAFWRSGQGPG
                     KPALVLETVETATS"
     gene            1881..3125
                     /locus_tag="VFU72_14730"
     CDS             1881..3125
                     /locus_tag="VFU72_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5424798.1"
                     /translation="MDLNYTPEDQAYRRQVRQWLADNLPEKRPETLEERRAWHQKLYE
                     AGFIGMGFPKRYGGQEARPMEQAIVGEELARANAPASLNGLAISIVAPTLIHHGTEEQ
                     KERYVQKILSAEEIWCQLYSEPNSGSDLASLQCRAEIDGDEFVINGQKIWTSGAYQSD
                     YGLMLARTDRNAPKHQGISNIIIDMHQPGVEVRPLKQISGSSEFCEVFFSDVRRNADN
                     LIGEMNAGWHIAQTTLGYERGGNALSRVTNHQTRFARLIEVVSALERNGTPALSDPLV
                     RQKLGHIYAEIEVLRYEALRILSRLEKGQRPGPESSIAKLHYSELDKRIQETTQEILG
                     PYGQLIEGEPDELALEPGAKFGDPGSWAFAFARSRGGTIAAGTSEIQKNILGERVLGL
                     PKEVRTDRIELQQAAANRGNAG"
     gene            3149..3658
                     /locus_tag="VFU72_14735"
     CDS             3149..3658
                     /locus_tag="VFU72_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5424799.1"
                     /translation="MSEVAGQERIEGAPPERVVRTVAELNELIGQDLTLGPWFEITQE
                     RVDLFADVTGDHQYIHVDPERARQTFFGGTVAHGFLTLSMGGLLGRGSQGIKVDLGGR
                     LTVNYGLNRVRFPAPVRVGKRVRQRSKLLNVEDIDGRAVQITRQQTIEIEGEDKPACV
                     AETLSRVYF"
     gene            3712..>5465
                     /locus_tag="VFU72_14740"
     CDS             3712..>5465
                     /locus_tag="VFU72_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5424800.1"
                     /translation="MQSAEMGESRGTAERTTFERAMRGLLHPRRIAIIGASPNRGFAS
                     SIQRRIVTCGYEGELFPVNPNYQEVMGLPAYPTLESIPGGADLAIVVVPSRLVFDVLD
                     SCERTGVKAINIITSGFAEKQEDETGPERQRAIRAFAERTGIRVVGPNCLGNVSVPNK
                     MVASSGQYQLPHRGPIALALQSGLLAYSLVIPQRARGLGFSYVVTLGNEADIDLADMV
                     QYYVDDEETRVIGCFVEQFRRPEAFLKAAGEAADRGKPIVMLKVGRSETARRAALAHT
                     GSLVGSDGVADAVMRQYGITRVFSLEEMTETLAIFHSRKLPGGRGASMAVASGGTAGV
                     VADIASDLGVDFPALQPETIAKIEAVIPPYGTVGNPLDYTGQAGSMPGVLEGCYAALA
                     EDPNIHVIMSGQAFPTVMDRTQSVGEAFTKLSRQHPDKVFLALALVPGEISMRLYDSD
                     PVEPMTDVDGVPILQGAEHGLKAVRALVNYAEFKRGWEGAQALAAEPSARAEEARALV
                     RAAGGQPLVERAAKAVLALYGLPVTQERLARSAEEAVDAAGAIGYPVVLKIESPDISH
                     KTDAGGVLLDLRDEDAVR"
BASE COUNT          838 a         1917 c         1883 g          827 t
ORIGIN      
        1 acctcggatt tgagccgccg tgacaagatc ctgcgcgaca agccggccaa cctcggcgat
       61 cgctgctatc tcaacccgga tgatccgagc gaggtcacgc ccggcacctg caccgacgcc
      121 ttgccgctgc acgccgatcc ccggcgagtg gccggagctc cgtttgtcga caacgtggtc
      181 aagtgcacgc tcaagccggt cgacttcggc gactaccggg cgtccttcga cgccgcgcag
      241 caggcacgcc tgcgggccat cttccccgac ggtgtctgtg actacagtca gccgggcgtt
      301 gggcaggtgc cgccggccgg cccctggatc tcgtacgggc cggctcctgg cctcacgctc
      361 tccggccacg cagccagcgc cccctgatgc tccgccccgt cgcgcctgct atagttatgg
      421 caggcgcgac ataaagctcg cggaggcgtg gtgcgagcgc gcgatggatg caccgcgctt
      481 ctatgatgcg cggtgcatcc atccggggaa tcacggcgcg ctcgccgtgg gggtcgagga
      541 agggaccggg atgtctgctt tcacgcgccg ggagcggctg atcgtcctcg cgttggcggt
      601 cgccgtcttc accggcacgc tcaatgtcta cctcctcacg ccctttctca agctgatcgc
      661 cgccgatttc ggcgtctccg aagcggccgc cggtcagttg tccacgacct acgcgctggc
      721 cgcgggcctg gttggactga tcgtcgcgcc gctggtcgac cgctatcagc ggcggcggct
      781 cctctatctc gccgtagcga tcatctcggt gtcgacggtc ttcacggcgg tggcgccctc
      841 gttcccggcg ctgctgttgg cccacggcct ggcgggcttc ggcgcggcct tcggcgccag
      901 ctcctgcttc gccatcgcca gcgatgcctt ccccgaccag cacaagcgca accgcagtct
      961 cgggctggtg atcgcggccg gcggcgtcag ctcggtcatc ggcgtgccga tcctgaccca
     1021 gatcgccgcc gccagtagtt ggcgctgggc ggtcgccgcg ctgctggtgc cctgtgtcgt
     1081 ggcgacgctg ctcatccggg tgctcccctc gcgcagcctc ggctcgcgca ccaccttcgt
     1141 gcgcgactat ctggccagct accggcgcgt gctgcacaac cacgagaccc gctggctcta
     1201 tctggcgacg ctcacgctgg cgaccgcgga gaccgccccc ttgatctaca gcgcctcgat
     1261 ctggcaggga ctcttcgatc tctcgctgga gagcttcggt tggatcttcc tgctcggcgg
     1321 cgtctgctac gcgatcggga gcaacctggc gccggtcctg ttgcggcggc gctcgccgcg
     1381 cgaggcgtgc atgctcaccg gagggctcca gttcctggcc gcgctcagct tcccgctggt
     1441 cgacagcagc ctgcccgccg cgctgttcgt ctacgccggc ccggtcgtga tgctgagcgc
     1501 gctcaacgtc gtcgcgctca atgtgctgct gcaggatagc ctgcccgccg accgcggcgc
     1561 ggtgctctcg ctcgacggga cgatcgccaa gtgcggcacc gcctgcgcgg ccatcttcgg
     1621 cggcattatc ctggcggcgg ccggcgaggg cgcgatcatc ccgttgatca atctggtgct
     1681 gccgttcacc ctgctggcct tctggcgcag cggtcagggc cccggcaagc cggcactggt
     1741 gctcgagacc gtcgagacgg ccacatcctg aggcgggcac ccgcgcaccc gatctgttga
     1801 cgacctccaa acgaagtcgt accgttgtcg cagcgcagac aatcgagctg gaggctgttg
     1861 ttacagggag ggttcgttcg atggacctca actacacgcc ggaagaccag gcgtaccggc
     1921 ggcaggtgcg gcagtggctg gccgataacc tgccggagaa acgtccggag accctggagg
     1981 agcgccgcgc ctggcaccag aagctctacg aggccggctt catcggtatg ggtttcccca
     2041 aacgctacgg cggccaggag gcgcgcccga tggagcaggc gatcgtcggc gaggagctgg
     2101 cccgcgccaa cgcgcccgcc agcctcaatg gcctggcgat ctcgatcgtg gcgccaaccc
     2161 tgatccacca cggcaccgag gagcagaagg agcgctacgt ccagaagatt ctctcggcgg
     2221 aggagatctg gtgccagctc tactccgagc ccaactccgg ctccgacctg gccagcctcc
     2281 agtgccgagc cgagatcgac ggcgacgagt tcgtcatcaa tggacagaag atctggacca
     2341 gcggcgccta tcagtccgac tacgggctga tgctggcgcg caccgaccgc aacgcgccca
     2401 agcaccaggg catctcaaac atcatcatcg acatgcacca gcccggtgtc gaggtgcgcc
     2461 cgctgaagca gatcagcggc agcagcgagt tctgcgaggt cttcttcagc gacgtgcgtc
     2521 gtaatgccga caacctgatc ggtgagatga acgccggctg gcacatcgcg cagacgaccc
     2581 tcggctacga gcgcggcggc aacgcgctca gccgggtcac caaccaccag acgcgcttcg
     2641 cccggctgat cgaggtggtc agcgccctgg agcgcaacgg cacgccggcc ctgtcggatc
     2701 cgctggtgcg ccagaagctg ggccacatct acgccgagat cgaggtgctg cgctatgagg
     2761 cgctgcgcat cctctcgcgg ctggagaagg gccagcggcc ggggccggag tcctcgatcg
     2821 ccaagctcca ctactccgag ctggacaagc gcatccagga gacgacccag gagatcctcg
     2881 gcccctacgg ccagttgatc gagggcgagc cggacgagct ggcgctggag ccgggggcca
     2941 aattcggcga tccggggagc tgggccttcg ccttcgcgcg ctcgcgcggc ggcaccatcg
     3001 ccgccggcac ctcggagatt cagaagaaca tcctgggcga gcgcgtgctc gggttgccca
     3061 aagaggtgcg cactgaccgc atcgagctgc agcaggcggc ggccaaccgg ggcaacgccg
     3121 gctagcgctt cagcggaagg ggggtgccat gagcgaggtc gccggccagg agcgtatcga
     3181 gggcgcgccg cccgagcggg tcgtgcgcac ggtcgccgag ctgaacgagc tgatcggcca
     3241 ggacctgacc ctcggcccct ggttcgagat cacccaggag cgggtcgacc tcttcgccga
     3301 cgtcaccggc gatcaccagt acatccacgt cgatcccgag cgcgccagac agaccttctt
     3361 cggcggcacc gtcgcccacg gcttcctgac gctctcgatg ggcgggttgc tggggcgggg
     3421 cagccagggg atcaaggtcg atctcggcgg gcgcctgacg gtcaactacg ggctgaatcg
     3481 ggtgcgcttc ccggcgccgg tgcgcgtcgg caagcgcgtc cgccagcgct cgaagctgct
     3541 caacgtcgag gacatcgacg gccgggcggt gcagatcacc cggcagcaga ccatcgagat
     3601 cgagggcgag gacaaaccgg cctgcgtggc cgagacactg agccgggtct acttctagct
     3661 gggcgacggg cgggagcgat ccccgcccgt cacgcataga gaggaagcgg catgcagtcg
     3721 gcggagatgg gggagagccg gggaacggcg gagcggacga ccttcgagcg ggccatgcgc
     3781 gggctgctcc atcccaggcg catcgccatc atcggcgcct cgcccaaccg cggcttcgcc
     3841 agcagcatcc agcggcgcat cgtcacctgc ggctacgagg gcgagctctt cccggtcaac
     3901 cccaattatc aggaggtcat gggcctgccg gcctacccga cgctggagtc gatccccggc
     3961 ggggcggacc tggccatcgt ggtggtgccg agccgcctgg tgttcgacgt gctcgactcc
     4021 tgcgagcgca ccggcgtcaa ggcgatcaac atcatcacca gcggcttcgc cgagaagcag
     4081 gaggacgaga ccggccccga gcggcagcgc gccatccgcg ccttcgccga gcgcaccggc
     4141 atccgcgtgg tggggccgaa ctgcctgggc aacgtcagcg tgccgaacaa gatggtcgcc
     4201 agttcggggc agtatcagct cccgcatcgc gggccgatcg cgctggcgct gcagagcggg
     4261 ctgctggcct acagcctggt catcccccag cgcgcccgcg gcctcggctt cagctacgta
     4321 gtcacgctcg gcaacgaggc cgatatcgac ctggccgaca tggtgcagta ctacgtcgac
     4381 gacgaggaga cgcgcgtcat cggctgcttc gtcgagcagt tccgccggcc ggaggccttc
     4441 ctcaaggcgg cgggcgaggc ggccgaccgt ggcaagccga tcgtgatgct caaggtcggg
     4501 cgctcggaga cggcgcgccg ggccgcgctg gcccacaccg gctcgctggt cggctccgac
     4561 ggcgtggccg acgcggtcat gcggcagtac ggcatcaccc gcgtcttcag tctggaggag
     4621 atgaccgaga cgctggccat cttccacagc cgcaagctgc ccggcgggcg cggcgcctcg
     4681 atggcggtgg cctcgggcgg caccgccggg gtggtggccg acatcgccag cgacctgggc
     4741 gtcgatttcc cggccctgca gccggagacg atcgccaaga tcgaggcggt catcccgccc
     4801 tacggcaccg tcggcaaccc gctcgactac accggccagg cgggcagcat gcccggcgtg
     4861 ctcgagggtt gctacgcggc gctggccgag gatcccaaca tccacgtcat catgtccggc
     4921 caggccttcc cgaccgttat ggatcggacc cagtcggtcg gcgaggcgtt caccaagctc
     4981 tcgcgccagc accccgacaa ggtcttcctg gccctggcgc tggttcccgg cgagatctcg
     5041 atgcggctgt acgactcgga tccggtcgag ccgatgaccg acgtcgatgg cgtgcccatc
     5101 ctgcagggcg ccgagcacgg cctgaaggcc gtgcgtgccc tggtcaacta cgccgagttc
     5161 aagcgcggct gggagggggc gcaggcgctc gcggccgagc cgagcgcgcg cgccgaggag
     5221 gcgcgggcgc tggtgcgcgc ggccggcggg cagccgctgg tcgagcgcgc ggccaaggcg
     5281 gtgctggcgc tctacgggct gccggtcacc caggagcggc tggcgcgcag cgccgaggag
     5341 gcggtcgacg ccgcgggcgc tatcggctac ccggtggtgc tcaagatcga gtcgccggac
     5401 atcagccaca agaccgacgc cggcggcgtg ctgctcgacc tgcgcgacga ggacgccgtg
     5461 cgcga
//
LOCUS       DASUBI010000755         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1123738, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000755 DASUBI010000000
VERSION     DASUBI010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123738"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="hemE"
                     /locus_tag="VFU72_14745"
     CDS             <1..870
                     /gene="hemE"
                     /locus_tag="VFU72_14745"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU5424801.1"
                     /translation="QPIERLGVDAAIIFADILLPLVPMGIELSFAAGEGPVIHNPVRS
                     ADDVRRLRLPEPEDDLAATLAAIRLVAGRLARGVPLIGFAGGPFTLASYLIEGGSSRN
                     YLRTKQLMYGDPASWDALMEKLGAMTAAYLRAQLAAGAEAVQLFDSWAGALSPRDYRE
                     RVLPHTRRIVEELQPLGAPVILFGTGTAGLLELLAESEADVIGLDWRIDLAAGWARVG
                     QQRAIQGNLDPALLFAPRDELARQVRAILEQAGGRPGHIFNLGHGILPETPPENVRYL
                     VELVHELGATGEG"
     gene            1030..1998
                     /gene="hemH"
                     /locus_tag="VFU72_14750"
     CDS             1030..1998
                     /gene="hemH"
                     /locus_tag="VFU72_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5424802.1"
                     /translation="MSQPKPGRTIGVLLMAYGGPNNLNEIPAYLLDVREGREPSPELI
                     AEMTHRYAAIGGRSPILELTRAQARGIERELNAGRAFERFHVSVGMRHWHPYIREVVP
                     QMLAAGVDEIVPVVMAPHYSRMSVGRYLQRVREALAAQGAEIPLRPLERWWDEPDFVA
                     AIAGRIEEGLEQFAPEERAGVYILFTAHSLPERILQWHDPYPEELRRSVEVVAEVVQP
                     ARYGFAFQSQGASSEPWLGPSVEDMLERLAAEGVRDVLLVPIGFVCDHVEVLHDVDIE
                     HRGQAEALGIRLERTASLNDAPLLCRAVAAAVRARLAQPAEAEAGA"
BASE COUNT          282 a          722 c          764 g          283 t
ORIGIN      
        1 cagccgatcg agcggctcgg cgtcgacgcg gccattatct tcgcggacat cctgctgccg
       61 ctagtcccca tgggcatcga gctctccttc gcggccggtg agggcccggt gatccacaac
      121 ccggtgcgct cggcggacga cgtgcggcgg ctgcggctgc cggagcctga ggacgatcta
      181 gcggcgacgc tggcggcgat ccggctcgtg gcggggcgcc tcgcacgggg ggtgccactg
      241 atcggcttcg ccggcggccc cttcaccctg gccagctacc tgatcgaggg cggctcctca
      301 cgcaactacc tgcgcaccaa gcagttgatg tacggcgatc ccgccagttg ggatgccctg
      361 atggagaagc tgggcgccat gaccgcggcc tatctgcggg cgcagctcgc ggcgggggca
      421 gaggcggtac agctcttcga tagctgggcg ggggcgctca gcccgcgcga ctaccgcgag
      481 cgcgtgttac cgcacacccg ccgcatcgtc gaggaattgc agccgctggg cgcgccggtg
      541 atcctcttcg gcaccggcac ggccggcctg ctggaattgc tggccgagag cgaggccgac
      601 gtgatcgggc tcgactggcg catcgacctg gccgccggct gggcgcgcgt cgggcagcag
      661 cgcgcgatcc aggggaatct cgacccggcg ctcctcttcg cgccgaggga cgagctggcg
      721 cgccaggtgc gcgccatcct ggagcaggcg ggcggccgac ccggccacat cttcaacctg
      781 ggccacggca ttctgcccga gacgccgccg gagaacgtgc gctacctggt cgagctggtg
      841 cacgagctgg gcgcgacggg ggagggatga cgccgcagag tgctcctgta gccgacgagg
      901 caggggggcg ggcaggcacg ggggcctgcc cctaccgcga tggggacacc cgtttcttgt
      961 aggggcggcc ccccgtggcc gccctccccg tggcccggcc gccgcccgca tggcgaggag
     1021 gagcaacgca tgagccagcc caaaccaggc cgcaccatcg gcgtgctgct gatggcctac
     1081 ggcgggccga ataatctgaa cgagattccc gcctacctgc tcgatgtgcg tgaggggcgc
     1141 gagccctcgc cggagctgat cgcggagatg acccaccgct acgccgccat cggcggccgc
     1201 tcgcccatcc tggagctgac ccgggcacag gcgcgcggca tcgagcgcga gctgaacgcg
     1261 ggccgcgcgt tcgagcgctt ccacgtctcc gtcggcatgc gccactggca cccctatatc
     1321 cgcgaggtgg tgccccagat gctggcggcg ggcgtcgacg agatcgtgcc ggtggtgatg
     1381 gcaccgcact actcgcgcat gagcgtcggg cgctacctgc agcgggtgcg cgaggcgctg
     1441 gcagcgcagg gcgccgagat cccgctacgg ccgctggagc gctggtggga cgagcccgac
     1501 ttcgtggccg ccatcgccgg gcgcatcgag gaggggctgg agcagttcgc gcccgaggag
     1561 cgtgcgggcg tctacatcct cttcaccgcc cacagcctgc ccgagcgcat cctgcagtgg
     1621 cacgacccct atccggagga gctgcgccgc tcggtcgagg tggtggccga agtggttcag
     1681 ccggcgcgct acggcttcgc cttccagagc cagggggcca gctccgagcc ctggctgggg
     1741 ccgagcgtcg aggatatgct ggagcggctg gcggccgagg gggtgcgcga cgtgctgctg
     1801 gtgccgatcg gcttcgtctg cgaccacgtc gaagtgctcc acgacgtcga tatcgagcac
     1861 cgcggccagg ccgaggcgct gggcatccgg ctggagcgca ccgcctcgct caacgacgcg
     1921 ccgctgctct gccgcgcggt cgccgccgcc gtgcgcgcgc ggctcgccca gccggccgaa
     1981 gccgaggcgg gagcctagca tggccacgac cacgcccagc gcgcacgcgg tcgtcgtcgg
     2041 gggcgggatc a
//
LOCUS       DASUBI010000756         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_687523, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000756 DASUBI010000000
VERSION     DASUBI010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687523"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFU72_14755"
     CDS             complement(<1..338)
                     /locus_tag="VFU72_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEU5424803.1"
                     /translation="MRPEEYLLIILRHWWVLLLAAAVAAGGAYLFTSREPVTYQASGR
                     LMAIAQPPDYWLDMYAKNRLASYQNLVGTWDFVSTALKEAKLNIDPGLASSELTLGHD
                     PNTNTIQIVVT"
     gene            complement(335..1033)
                     /locus_tag="VFU72_14760"
     CDS             complement(335..1033)
                     /locus_tag="VFU72_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEU5424804.1"
                     /translation="MELRAYLAVLARRWQLVALVPALVLLAIIAQIIQTKPAYTATTQ
                     VSVIRNEEQPQLTDRFKYDGYYALLASEYALDDLVKTVRGNVFAQDVAARIHQDTGQD
                     ISAGEIQQAITSSRLNRILTINATTSDPKRAVLIASAAAKTLEAKGLEYFQPNGPSSA
                     SIETIDAATEAHPDTLKRALLYGLQLVVAIFAGLVLAFLVDYLDDTLRGAEAVSGALD
                     LPVVGLIPDGGVPR"
     gene            complement(1024..2199)
                     /locus_tag="VFU72_14765"
     CDS             complement(1024..2199)
                     /locus_tag="VFU72_14765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424805.1"
                     /translation="MAALDPSRSATPARVALVHDYLNQYGGAERVLEVFHDLYPNAPV
                     FTSIYAPELMPPAYRAWDVRVSFMQRLPGIHRHHQPYLPLYPLAFRRLRLDGYDLVLS
                     DSSAFAKGIPVPPSAIHVCYVHSPMRFAWDFARYADRERLGGALRRALPPLLTGLRRW
                     DIASARGVDYLIANSSAVARRIAAYWSREATVIYPPVETEAGEPVAPGQVEDYFLLVS
                     RLVPYKRFDVVIDAFNALGLPLKIVGEGRARPELERRAGPNVQFLGAISDAEKYRLYA
                     RCQAALFPAEDDFGIAQVEVQAAGRPAIALAAGGALDTVIDGQTGVLFPEQSPAAVIA
                     AVRRFQSLSFAAEPIARHAARFSRARFAAEIATFVSSCLEAGKVRGRALAEARPSWN"
     gene            complement(2308..2940)
                     /locus_tag="VFU72_14770"
     CDS             complement(2308..2940)
                     /locus_tag="VFU72_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424806.1"
                     /translation="MTLLFASCQGSSSTQTPAGAAPTSSAVAVSTATAVTASTGTTAG
                     NTSTAAPHATAAPTATKPPPPAPTRSATAAPTASATTPTPLVAPTLTAAQTKAEYRTG
                     LSVRDLTNHIDRYHDWKLFYQGTVLNVSPEANGDVFMQVRIPFGEGANDTEDVVYKIP
                     AGSNIAVEGLARDSKVALWGRPQQMTTVTNALGQQVSEPLLLLDFLQKLG"
BASE COUNT          545 a         1118 c         1266 g          551 t
ORIGIN      
        1 gtgaccacga tctgaatcgt attggtgttc gggtcgtggc ccagggtcag ctcgctgctg
       61 gccagccccg gatcgatgtt gagcttggcc tccttgagcg cggtcgagac gaagtcccag
      121 gtgccgacca gattctggta cgaggcgagc cggttcttgg cgtacatgtc gagccagtag
      181 tcgggcggtt gggcgatcgc catcagccgc ccgctggcct gataggtcac cggttcacga
      241 ctggtgaaga ggtacgcgcc gccggccgcg accgccgccg ccagcagcag cacccaccag
      301 tggcgcagga tgatcaggag atactcttcc ggtctcatcg cggaactccg ccatcgggga
      361 tcagccccac caccggcagg tcgagcgcgc cgctgacggc ctcggctccc cgcagggtgt
      421 cgtcgaggta gtcgaccagg aaggccagca ccagcccggc gaagatggcg accaccagct
      481 ggagcccgta gaggagggcg cgcttgagtg tgtcggggtg ggcctcggtg gcggcgtcga
      541 tcgtctcgat cgaggcggag gaggggccgt tcggctggaa atactctaat cccttggcct
      601 ccagcgtctt ggcggccgca gaggcgatca gcacggcacg cttcgggtcg gaggtggtgg
      661 cgttgatcgt caagatgcgg ttcaagcggc tcgaggtgat cgcctgctgg atctcgccgg
      721 ccgagatgtc ctggccggta tcctggtgga tgcgcgccgc cacatcctgg gcgaagacat
      781 tgccgcgcac cgtcttgacc aggtcgtcga gcgcgtattc gctggccagc agcgcgtagt
      841 agccgtcgta cttgaagcgg tcggttagtt gcggctgctc ttcgttgcgg atgaccgaca
      901 cctgggtggt ggcggtgtag gccggtttgg tctggatgat ctgcgcgatg atcgcgagca
      961 gcaccagcgc cggcaccagc gcgaccagct gccagcgccg cgccagcacg gcgagatagg
     1021 ctctcagttc cacgacgggc gtgcctccgc cagcgcccgg ccgcggactt tgcccgcctc
     1081 caggcaactg ctgacaaacg tggcgatctc cgcggcgaag cgcgcgcggc tgaagcgtgc
     1141 ggcgtggcga gcgatcggct cggccgcgaa cgacaacgac tgaaaacgcc gcaccgcggc
     1201 aatgaccgcg gcgggcgatt gctccgggaa gagcacgccg gtctggccgt cgatgaccgt
     1261 gtcgagcgcg ccgccagcgg ccagcgcgat cgccgggcgc ccggcggcct gcacctctac
     1321 ctgcgcgatg ccgaagtcgt cttcggcggg gaagagcgcg gcctggcagc gcgcgtagag
     1381 gcggtacttc tcggcgtcgg agatggcgcc gaggaactgg acattcggcc ccgcgcggcg
     1441 ctccagctca gggcgggcgc ggccctcacc gacgatcttg agcggcagcc ccagcgcatt
     1501 gaaggcgtcg atcaccacgt cgaagcgctt atagggcacc aggcgcgaga cgagcaggaa
     1561 gtagtcttcg acctggccag gagctaccgg ctcgcccgcc tcggtctcga ccggagggta
     1621 gataacagtg gcctcgcggc tccagtaggc ggcgatgcgc cgcgccacgg cgctggagtt
     1681 ggcgatcagg tagtcgacgc cgcgcgcgct ggcgatatcc cagcggcgta gcccggtgag
     1741 cagcggcggc agggcccgcc gcagcgcgcc gcccagccgc tcgcggtcgg cgtagcgcgc
     1801 gaagtcccag gcgaagcgca tcgggctgtg tacgtagcag acgtggatgg cggagggcgg
     1861 caccggtatc cccttggcga aggcgctgct gtcgctgagc accagatcgt aaccgtccag
     1921 ccgtaggcgg cgaaaggcga gggggtagag cggcaggtag ggctggtggt ggcggtggat
     1981 accagggagc cgctgcatga aggagacgcg cacgtcccag gcgcgatagg ccggtggcat
     2041 cagctccggg gcgtagatcg aggtgaagac cggcgcgttg gggtagaggt catggaagac
     2101 ctcgagcacg cgctcggcgc cgccgtactg attgaggtag tcgtgtacca gcgcgacccg
     2161 cgccggggtg gcgctgcgcg acggatccaa agctgccatg cgcgtaagga tacacgcttg
     2221 gtcgcggagt agcagccggt tccggcccat accggcgcgc gaaatgggcg tcacggcgcg
     2281 agccgccacc ggcttgtgtc ccgcgcctca gcccagcttt tgcaggaagt ccaacagcag
     2341 aagcggctcg ctcacctgct gccccagggc gttggtcacg gtcgtcatct gctgtggccg
     2401 cccccagagg gcaaccttcg agtctcgggc gaggccctcc accgcgatgt tcgacccggc
     2461 cggaatcttg tagacgacgt cctcagtatc gttcgccccc tccccgaagg ggatacgcac
     2521 ctgcatgaag acatcgccgt tcgcctccgg cgagacgttg aggacggtcc cctgatagaa
     2581 gagcttccag tcgtgataac ggtcgatatg gttggtgaga tcgcgcacgc tgaggccggt
     2641 acggtactcg gccttggtct gggcggcggt gagcgtcggt gcaaccagcg gagtcggcgt
     2701 ggtggccgac gctgtgggcg ccgccgtcgc ggatcgtgtc ggcgccggag gtggtggctt
     2761 ggtcgctgtg ggtgcggccg tcgcatgagg cgcggccgtt gaggtgttgc cggccgtcgt
     2821 tccggtggac gcggtgacgg ccgtcgccgt gctcaccgcg acggccgacg atgttggcgc
     2881 ggcccccgcg ggagtctggg tactggagct gccctggcag ctcgcgaaca acaacgtcat
     2941 gatggcaacc gcgaggattc cacgcattcg ccccaccgat catgtgcctg gcccggtcgt
     3001 cacgccagtc ttcggctcca tcgtcccgat ccgcgggctc ccttgccgca agactcgagc
     3061 cagctcggac aggggccccg gtcagaccgg gcccgcaggt gctgatcgtt ggggcgagcc
     3121 acggcgtggc gaaggggatt cggcagggtg ggttacggac ccgccgattt cgttgggtgg
     3181 cgcggaggac ggatccttcg actgcgctca ggatgacagg gttaacaggg ggctgacgag
     3241 gacgaccatc gtattcgtcc gccccaatcc cccaaacatg tcctcctgag cttgtcgaag
     3301 gatccgtgcc ccacgcggca cttcacacgg ttgatcacag cctcagcggc gcgcgcccca
     3361 gccgccggcg ccgaccggct cggcctcggg ctccagctcc aacgcgccgg ccggaaccag
     3421 ctcgggttcg cgggtgcgcg gagccatcga gggcgccgcg tcctggtcgc ggatcaggaa
//
LOCUS       DASUBI010000757         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_687532, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000757 DASUBI010000000
VERSION     DASUBI010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687532"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFU72_14775"
     CDS             <1..899
                     /locus_tag="VFU72_14775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477550.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5424807.1"
                     /translation="GGGGRGMRIAWDDQELARVLPLAQSEAQSAFGDSGVYIERYLER
                     PRHVEVQILADQHGNVVALGERDCSLQRRYQKLVEESPAPNVSSRLRENLAKSATRAA
                     KSIGYTGAGTFEFLVDRDEHFYFTEVNARIQVEHPVTELVTGLDLVKWQIRVAAGQPL
                     GFEEKDVRPRGHAIELRINAEDPLRDFAPSAGQVSGLLLPGGPGVRVDTHLYPGYVVP
                     PHYDSLLAKLIVWGATRQEAIDRLRRALLETVISGIDTTVPFYQQLVEDGAFGSGQVH
                     TGFVTEFLARNAAPVLNPVASD"
     gene            1086..1577
                     /gene="nusB"
                     /locus_tag="VFU72_14780"
     CDS             1086..1577
                     /gene="nusB"
                     /locus_tag="VFU72_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5424808.1"
                     /translation="MSVALTRHQARTLALQVLFEVDAAGHQASDVLARYMADLALPQP
                     VRRYVERLVTGVMQHLDDIDRQIGEAAPAFPVGQLPAVDRNILRIAIYELQQEPDVPV
                     KAAINEAVELAKRFGGENSSRFVNGVLGTIASRLPPARMQPVAETERGRHNNESLGDG
                     GSR"
     gene            1592..1834
                     /gene="acpP"
                     /locus_tag="VFU72_14785"
     CDS             1592..1834
                     /gene="acpP"
                     /locus_tag="VFU72_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5424809.1"
                     /translation="MDSTFERVQGVVSEQLGVEPEKVTLDAEFVQDLNADSLDLVELI
                     MQLEEEFGIEISDEQAESIQTVGDAVEFIQEHMAAK"
BASE COUNT          343 a          646 c          761 g          310 t
ORIGIN      
        1 ccggaggcgg cgggcgcggc atgcgcatcg cctgggacga ccaggaactc gcgcgcgtgc
       61 tgccgctggc gcagagcgag gcccagtccg ccttcggcga cagcggggtc tacatcgagc
      121 gctatctgga gcgtccgcgc cacgtcgagg tgcagatcct ggccgaccag cacggcaacg
      181 tggtggcgct gggcgagcgc gactgctcgc tgcagcggcg ctaccagaag ctggtcgagg
      241 agtcgcccgc gcccaacgtc tcctcgcgcc tgcgcgagaa cctggccaag tcggccacgc
      301 gggcggccaa gtcgatcggc tacaccggcg ccggcacctt cgagttcctg gtcgaccgcg
      361 acgagcactt ctatttcacc gaggtcaacg cgcgcatcca ggtggagcac ccggtcaccg
      421 agctggtcac cggcctcgac ctggtcaagt ggcagatccg ggtggcggcc ggtcagccgc
      481 tcggcttcga ggagaaggac gtgcggccgc gcgggcacgc catcgagctg cgcatcaacg
      541 ccgaggatcc gctgcgcgat ttcgccccca gcgccggcca ggtcagcggg ctgctgttgc
      601 cgggggggcc gggggtgcga gtggataccc atctctatcc cggctacgtg gtgccgcccc
      661 actacgactc gctgctggcc aagctgatcg tctggggtgc cacgcgccag gaggcgatcg
      721 accgcctgcg ccgggcgcta ctggagacgg tgatcagcgg gatcgacacg accgtgccct
      781 tctaccagca gttggtcgag gatggcgcct tcggcagtgg gcaggtgcac accggtttcg
      841 tgaccgagtt cttggcccgc aacgccgcgc cggtgctgaa tccggtggcc agcgactgag
      901 gggtgcgctt gcggcggcct cggaacggat gttgcaggcc ggggcggggt gcccgatggg
      961 gagccctcat ccccaaccct tctcccgcgc atcgggagaa gggagacgaa ggatttgtag
     1021 gggcgggttt cgtgcccgtc cgccgcgctg cccggatgcg gcgtcggtag aaatggcgag
     1081 gaacgatgag tgtcgcgctg acccgtcacc aggcgagaac gctggcgctc caggtgctct
     1141 tcgaggtcga cgcggcgggg catcaggcga gcgacgtgct ggcgcgctac atggccgacc
     1201 tggcgctgcc gcagccggtg cggcgctacg tcgagcggct ggtgaccggg gtgatgcagc
     1261 atctggacga tatcgaccgg cagatcggcg aggcggcgcc ggcctttccg gtggggcagt
     1321 tgccggcggt cgaccgcaac atcctgcgca tcgcgatcta cgagctgcag caggagccgg
     1381 acgtgccggt caaggccgcg atcaacgagg cggtggagct ggccaaacgc tttgggggcg
     1441 agaactcgag ccgcttcgtc aacggcgtac tcggcaccat cgcctccagg ctgccgcccg
     1501 cgcgcatgca gccggttgcc gagactgaac gcggcaggca taataacgaa tcattagggg
     1561 atggcggaag ccgatagtgg gaggtaccag ggtggactcg acctttgagc gtgtgcaggg
     1621 cgtcgtttcg gagcaactgg gggttgagcc ggagaaggtg acgctcgacg cggagttcgt
     1681 gcaggatctc aacgcggatt cgctcgatct ggtcgagctg attatgcagc ttgaggaaga
     1741 gttcggcatc gagatctcgg acgagcaggc cgagagcatt cagacggtgg gcgacgcggt
     1801 cgagttcatc caggagcaca tggcagccaa gtagtctgac ggctcaatac cggccgccgg
     1861 gtcttgggga cgcccggcgg aggtgaggcc gacggcgggg cggccgcgag tcagctcaca
     1921 cgccttagca gagcagggac catggtcagc atcatcgatc gcttgcgcgg agacaacgtc
     1981 cccgcaccgc tcgggccggc cgagccggag ccggaggaac aattcgccga gatgaccctc
     2041 caggagcacc tggaggagct
//
LOCUS       DASUBI010000758         7747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1236710, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000758 DASUBI010000000
VERSION     DASUBI010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236710"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFU72_14790"
     CDS             <1..807
                     /locus_tag="VFU72_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5424810.1"
                     /translation="LRPVYLTVDLNLPRAITRAQLAELWEAVDQSCRELGIAIVTGHT
                     ARYDGCNYPMVGGATIISLGARDAYVTPAMAQVGDAVIVTKGAAIEATGLFGVTFPQR
                     ISQALGPQVARAAEELFYQMTVVKDAMTAVEVGVRERGVTAMHDATECGVWGGLYEIA
                     EASGVGMVIDQAAVPVAPAARAICDYFGMDPYSAISEGTLLLTCRPQAAQAVLDRLSG
                     AGITASRVGEVVPAAHGVRLVEHGQERPLAHPRVDPFWEAYAKALSEGAG"
     gene            1033..1287
                     /locus_tag="VFU72_14795"
     CDS             1033..1287
                     /locus_tag="VFU72_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424811.1"
                     /translation="MNGLIEQYIEQNPYKPGADEVRLIGYGVSVWALIAYLHAAGGDL
                     QRAARAYDVPLAALQAAVAYYEQHREIIDARIAANAPAAA"
     gene            1296..1673
                     /locus_tag="VFU72_14800"
     CDS             1296..1673
                     /locus_tag="VFU72_14800"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU5424812.1"
                     /translation="MARFYLDHNVSLDTATELRLRHHQATTARALGLERASDAEQLLA
                     AVHLGAILVTHNAKDFILLNDAWNRWARAWQVNARHEGIIVTPDTWLEPHEARELSDF
                     LVGGYPLPNQIYVWQSRAGWVAK"
     gene            complement(1816..2322)
                     /locus_tag="VFU72_14805"
     CDS             complement(1816..2322)
                     /locus_tag="VFU72_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5424813.1"
                     /translation="MTTVPPLETARLLLPPFTLEDAHAVLADSRVGRAWSPGYPTEGD
                     REIAAMLLRTGPPSDQRAVVFGTRRIVLRASGLTVGGIGFFGPPDEAGTLSVGYGIAP
                     EVRGQGLTSEALRAMLAFAATQPEVRRVLADTTPDNLASQRVLAKAGFQRVGADEALI
                     YYEYAIAR"
     gene            complement(2510..4390)
                     /gene="treZ"
                     /locus_tag="VFU72_14810"
     CDS             complement(2510..4390)
                     /gene="treZ"
                     /locus_tag="VFU72_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEU5424814.1"
                     /translation="MDRGVGVPVGALYEGEGRCRFRVWAPRARAVALALQAPESRLVL
                     LEARARGYWEALVEGVRPGARYKFRLDGQREYPDPAARSQPEGVHGPSEVTDPAAFPW
                     SDRDWSPPARARLVIYELHVGTFTPQGTFDAVIPRLGYLRELGVNAIELMPVAQFPGG
                     RNWGYDGVYPYAAQNSYGGPAGLARLVDACHHAGLAVFLDVVYNHLGPEGNYLAQFGP
                     YFTERYHTPWGAAVNVDGPESDEVRNYFVESALYWLEALHVDGFRLDAIHAIVDQSAV
                     PFLRQLAGLVHQRAAELGRHVELIAESDLNDPRVVQGAPLGGLGFDAQWADDFHHALE
                     AQLIGARSPYIADYGSFGALARALRQALVYTGQYAPRRRRHFGAQPEQTAADQYVVFI
                     QNHDQVGNRAHGERLGQLVPLARRKLAACAYLLAPFLPLLFMGEEYGEPHPFLYFTSH
                     HDQRLVEAVRRGRKAEFAHFTGQAEPPDPQAVETFARSKLQLDLHTQGEHAALFRLYQ
                     ELLRLRRALPALAKLSKDDLEVVSRAPDKLLAVHRWDGPSHAVLVLNLSDEASTLALE
                     LPGGRWAKRLDAAAPRFGGPGASAPDGFEANPFATLELAPWATAVYELEAVEDRLPAE
                     DA"
     gene            4674..5027
                     /locus_tag="VFU72_14815"
     CDS             4674..5027
                     /locus_tag="VFU72_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424815.1"
                     /translation="MSRRHAKAHQHAVHQQHQESTSRPAEEDAVAEWGEWLDHQYDPG
                     YYTGGRIPPFLRNRGGSPLFGYLLLIFGGLGLLLGIAGLAGGQPASGLLVEIVISAAM
                     AAAGWRLSRGRGRGR"
     gene            complement(5041..5949)
                     /locus_tag="VFU72_14820"
     CDS             complement(5041..5949)
                     /locus_tag="VFU72_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU5424816.1"
                     /translation="MATHHFQPTHYHNTLGSHEPVLRISPGDTVITTTVDAAGQDASG
                     QRVTTPGNPQTGPFYLEGAEPGDGLVLRIQRLWPNRDYGIGASVVSENVVDPHFVREL
                     PESMTLQWAIDRQAGTTSVETDSPLGRLELALDPMLGCFGVAPWGGQAVSTGTSGEWG
                     GNMDYRGFRPGMTIYLPVFVPGALLHVGDGHGVQGDGEIVGTGVEISCDIEFTVDLRK
                     NWEIHWPRGENDDFIFAVGNARPLDQALQHATTEMMRWLAADYGLDARAASTLLGQTV
                     VYDLGNVYDPAYTMVCKVPKAVLPAR"
     gene            complement(5968..6939)
                     /locus_tag="VFU72_14825"
     CDS             complement(5968..6939)
                     /locus_tag="VFU72_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5424817.1"
                     /translation="MVQTGREPSRLPTYDELPVTPGNPPLCAWGLWGERDELGMLNLL
                     TPERVARAARLARSGRRFGLSWSMELPEPPLFFRQRSEHAVLNKRPFVNDDFYTALNP
                     QSSSHWDGFTHYGNAEHQCFFMGVQPAEITGRPGTRNSIHVWAERGIAGRGVLIDYRR
                     WAEANGITYSPGEHEAITVAGLLAAAGDQGVQFETGDILLVRTGFTAWYESLTAEARA
                     ALAKGPHTFAGVAQGHESLRFLWDNHFAAVGGDAPSFEAWPPTPGMQALHPTLLPLWG
                     MPIGEMFNLEALAADCARDGVYEFLLTSEPLNKLGGVATPANALAIK"
     gene            complement(7039..7440)
                     /locus_tag="VFU72_14830"
     CDS             complement(7039..7440)
                     /locus_tag="VFU72_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424818.1"
                     /translation="MEARPDAGIDRNARFTLEEAAFLTHTLWSLPQRAPLPLVDEVRL
                     ALDTAPAAEGPEQRWGFERENLLRKLARLTPFQQQAIRDAIDIRRQALPPADLARVEA
                     ILDNWARLKSEHEQREFLRARLHAIGLPLES"
     gene            7588..7660
                     /locus_tag="VFU72_14835"
     tRNA            7588..7660
                     /locus_tag="VFU72_14835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7621..7623,aa:Thr,seq:cgt)
BASE COUNT         1150 a         2649 c         2807 g         1141 t
ORIGIN      
        1 ctgcggccgg tctacctcac cgtcgatctc aacctgccgc gcgccatcac gcgcgcgcaa
       61 ctggccgagc tgtgggaagc cgtcgaccag agctgccgcg agctgggcat cgccatcgtg
      121 accggccaca ccgcgcgcta cgacggctgc aactatccca tggtcggcgg cgccaccatc
      181 atcagcctgg gggcgcgtga cgcctacgtc acgccggcca tggcccaggt cggcgacgcg
      241 gtcatcgtga ccaagggcgc ggccatcgag gccaccggcc tcttcggcgt caccttcccc
      301 cagcgcatca gccaggcgtt ggggccccag gtggcgcgcg cggcggagga gctcttctac
      361 cagatgacgg tggtcaagga cgccatgacc gcggtcgagg tgggcgtgcg cgagcgcggc
      421 gtgaccgcga tgcacgacgc caccgagtgc ggcgtctggg gcgggctcta cgagatcgcg
      481 gaggcttcgg gcgtaggcat ggtgatcgac caggcggcgg tgccggtggc gccggcggcg
      541 cgcgccatct gcgactactt cggcatggac ccgtacagcg ccatcagcga ggggacgctg
      601 ctcctcacat gccggccgca ggcggcccag gccgtgctcg accggctgag cggagccggc
      661 atcaccgcca gccgcgtggg cgaggtcgtg cccgccgcgc acggggtgcg gctggtggag
      721 cacggacaag agcgcccgct ggcccacccg cgcgtcgacc ccttctggga ggcctacgcc
      781 aaagcgctca gcgaaggagc gggttagcgc gccgctgcag acaagctgca agccgttgag
      841 gccgagctcg catccgtggc agggctgcct catgcgtgcg cgggtgactt caggcatact
      901 cgtggtgaag gtgagccacg tacttgatct ggactacggg gcacttgagc gcgcggccgc
      961 tggcgttaca ttgggcacag ggtttgcgcg gtggctctgc ctgcgggcgt cgtgtccgag
     1021 agcgaggcag cggtgaacgg gctgatcgaa caatacatcg agcaaaaccc ctacaagccg
     1081 ggagccgatg aggtgcgcct tattggttac ggcgtgtccg tctgggcact catcgcctac
     1141 ctccacgcgg ccggcggcga tctccagcgc gccgctagag cgtacgacgt gccgctcgcg
     1201 gctctccagg ccgcggtcgc ctactacgag cagcatcgcg agatcatcga tgctcgcatc
     1261 gcggcaaacg cgcctgccgc agcctagctc acgcggtggc gcgtttctac ctcgaccaca
     1321 atgtctcgct tgatacggcg accgagctcc ggctccgcca tcaccaggca acgacggcgc
     1381 gcgcgctcgg tttggagcgc gcatcggacg cggaacagtt gctcgccgcc gttcacctgg
     1441 gggcaatcct ggtcacccac aacgccaaag acttcatctt gctgaacgac gcctggaacc
     1501 gctgggcgcg ggcctggcag gtcaacgcgc gccacgaggg aatcatcgtc acccccgata
     1561 cctggctgga gccacatgaa gcacgagaac tgagtgactt cctcgtaggc ggctatccct
     1621 tacccaatca aatctatgtc tggcagtcgc gggccggctg ggttgcgaag tagctgaacc
     1681 ggcgctcccc atcctctgaa tgggagccgc gcagggtgtg cggctgatgg agcacggaca
     1741 agagcgcccg ctggcccacc cgcgcgtcga ccccttctgg gaggcctacg ccaaggcgct
     1801 cagcgaagga gcgggttagc gcgcgatggc gtactcgtag tagatcagcg cctcatcggc
     1861 gccgacgcgc tggaagccgg ccttggccag tacgcgctgc gaggcgaggt tgtcgggggt
     1921 ggtgtcggcc agcacgcggc gcacctcggg ctgcgtggcg gcgaaggcca gcatggcgcg
     1981 tagcgcctcg ctcgtcagcc cctgcccgcg cacctcgggg gcgatgccgt agccgacgct
     2041 gagcgtgccg gcctcatcgg gcgggccgaa gaagccgatg ccgccgacgg tcaggccgga
     2101 ggcgcgcagc acgatccggc gcgtgccgaa caccaccgcg cgctgatccg atggcgggcc
     2161 ggtgcgcagc agcatggcgg cgatctcgcg gtcgccctcg gttgggtagc ccggcgacca
     2221 ggcgcgcccc acacggctgt cggcgagcac ggcgtgcgcg tcctccagag tgaagggcgg
     2281 cagcagcagg cgcgcggtct ccaggggcgg gacggtggtc acggggcctc cttgcctttc
     2341 gacaacgtta ggtgccgggc gaggacggat ccctcgacag gctcgggatg acatgcatgg
     2401 gggatggggg cgaacgacga cgatttgcgt cgcctgacac aggtcgtcgt acgcccctcc
     2461 cttcccctgt catcccgagc gctgtcgagg gatcagtctt gcctggcgtc tatgcatcct
     2521 ccgcaggtag ccgatcctcg accgcctcca gctcgtagac cgcggtggcc cagggggcga
     2581 gttcgagcgt ggcgaacggg ttggcctcga acccgtccgg agcggatgcg ccgggaccgc
     2641 cgaagcgtgg cgcggccgcg tcgagccgct tggcccagcg accacccggc agctccagcg
     2701 ccagcgtgct cgcctcgtcg ctcaggttca gcacgagtac agcgtggctg gggccgtccc
     2761 agcggtgcac ggcgagcagc ttgtccggtg cccggctgac gacctccaga tcatccttgc
     2821 tgagtttggc cagcgccggc agcgcgcggc gcaggcgcaa gagctcctgg tagagccgga
     2881 agagggcggc gtgctcacct tgcgtgtgca ggtcgagctg gagcttcgag cgcgcgaagg
     2941 tctcgacggc ctgcggatcg ggcggctcgg cctggcccgt gaagtgggcg aactcggcct
     3001 tgcgcccgcg ccgcaccgcc tcgaccagcc gctggtcgtg gtggctggtg aagtagagga
     3061 aggggtgtgg ctcgccgtac tcctcgccca taaagagcag cggcaggaag ggcgcgagga
     3121 gataggcgca ggcggcgagc ttgcgccggg ccagcggcac gagctgcccc aagcgctcgc
     3181 cgtgggcgcg gttgccgacc tgatcatggt tctggatgaa gaccacgtat tggtcggccg
     3241 cggtctgctc cggctgggcg ccgaagtggc gccgtcggcg cggggcgtac tggccggtgt
     3301 agacgagggc ctggcgcagg gcacgcgcca gagcgccgaa ggagccgtaa tcggcgatgt
     3361 aggggctgcg cgcgccgatc aactgcgcct ccagcgcgtg gtggaaatcg tcggcccact
     3421 gggcgtcgaa gccgagtccg cccagtggcg cgccctggac cacgcgcggg tcgttgaggt
     3481 cgctctcggc gatcagctcg acgtggcgcc ccagttcggc ggcgcgctgg tggaccagcc
     3541 cggcgagctg ccgcaggaag ggcacggccg actgatcgac gatggcgtgg atcgcatcca
     3601 ggcgaaagcc gtcgacgtgc agcgcctcca gccagtagag cgcgctctcg acgaagtagt
     3661 tgcggacctc atcgctctcc ggcccgtcga cgttgaccgc cgcgccccag ggcgtgtggt
     3721 agcgctcggt gaagtaggga ccgaactggg ccagatagtt cccctccggg ccgaggtggt
     3781 tgtagaccac gtccaggaag accgccagcc ccgcgtggtg gcaggcgtcg accaggcgcg
     3841 ccagcccggc cgggccgccg tagctgttct gcgcggcgta ggggtagacg ccgtcgtagc
     3901 cccagttgcg cccgccgggg aactgcgcca ccggcatcag ctcgatggcg ttgacgccga
     3961 gctcccgcag atagcccagg cgcgggatga cggcgtcgaa ggtgccctgg ggcgtgaagg
     4021 tgccgacgtg cagctcgtag atgacgagcc gcgcgcgcgc gggcggcgac cagtcccggt
     4081 cgctccaggg aaaggcggcg gggtcggtca cctccgacgg cccgtggacg ccctccggct
     4141 ggctgcgcgc ggccgggtcg gggtattcgc gctggccgtc gaggcgaaat ttgtagcgcg
     4201 cgccgggccg gacgccctcg accagcgcct cccagtagcc gcgcgcgcgc gcctccagca
     4261 ggacaaggcg gctctccggc gcctgcagcg ccagcgccac ggcgcgcgcc ctcggcgccc
     4321 agacgcggaa gcgacagcgc ccctcgccct catacagcgc ccccaccggc acgccgactc
     4381 cgcggtccat tgtgtttccc ctcggtgaaa agagcccatc gaagacgttc ggtctcgcgt
     4441 gcggcacgga tccttcgaca agctcaggat gacagggtga gtaaggggct gacgacgacg
     4501 atctgcgtcg tccggtaggc ctcatcccct ccccttgtca tcctgagcgc agtcgaagga
     4561 tccgtcctca cccggcacca atcctcgtcg tagggcaccg cacggcgctc gccagcgcgc
     4621 ctcccatgcg agataatgcg caaagatccg atgcagcgaa cggggggagc gcgatgagcc
     4681 gccggcacgc gaaggcgcac cagcacgccg tgcatcagca gcaccaagaa tccacgagcc
     4741 ggcccgccga ggaagacgcg gtggccgagt ggggcgagtg gctcgaccac cagtacgacc
     4801 ccggctacta caccggcggg cgcatcccgc ccttcctgcg caaccggggc ggctcgcccc
     4861 tcttcggcta cctgctgctg atcttcggcg gcctcggcct cttactcggc atcgccggcc
     4921 tggcgggtgg tcagcccgcc agcggcctgc tcgtcgagat cgtcatcagc gcggccatgg
     4981 ccgccgccgg ctggcgactg agccgcggac gggggcgagg acggtagggg cgcatctccc
     5041 ctacctggcc ggcagcaccg ccttcggcac cttgcagacc atggtgtagg cgggatcgta
     5101 gacgttgccc aggtcgtaaa cgacggtctg gccgagcagc gtgctggcgg cgcgggcgtc
     5161 gaggccgtag tcggcggcca gccagcgcat catctcagtg gtggcgtgct gcagcgcctg
     5221 gtcgagcgga cgcgcgttgc cgacggcgaa gatgaaatcg tcgttctcgc cgcgcggcca
     5281 gtggatctcc cagttcttgc gcaggtcgac cgtgaactcg atgtcgcagg agatctccac
     5341 cccggtgccg acgatctcgc cgtcgccctg caccccgtgg ccgtcgccga cgtggagcag
     5401 cgcgccgggc acgaagaccg gcaggtagat cgtcatgccg gggcggaagc cgcggtagtc
     5461 catgttgccg ccccactcgc ctgaggtgcc ggtcgagacc gcctggccgc cccagggcgc
     5521 gaccccgaag cagccgagca tcgggtccag cgccagctcc agccggccca gcggcgagtc
     5581 cgtctcgacg ctagtggtgc cggcctgacg gtcgatggcc cactggaggg tcatactctc
     5641 cggcagctca cgcacgaagt ggggatcgac cacgttctcg gagaccacac tggcgccgat
     5701 gccgtagtcg cggttgggcc agaggcgctg gatgcgcagc accagcccgt cgcccggctc
     5761 ggccccttct agataaaacg gccccgtctg cgggttgccc ggcgtggtca cgcgctggcc
     5821 gctggcgtcc tgcccggcgg cgtcgacggt ggtggtgatg accgtgtcgc ccggggagat
     5881 acgcagcacc ggctcgtggg agccgagcgt gttgtggtag tgcgtgggct ggaagtgatg
     5941 cgtcgccatg cggccctctc ctccttgcta cttgatcgcc agcgcgttgg cgggggtggc
     6001 cacgccgccg agcttattga gcggctccga ggtcagcagg aactcgtaga cgccgtcgcg
     6061 ggcgcagtcg gcggccaggg cctccaggtt gaacatctcg ccgatcggca tgccccagag
     6121 cggcagcagg gtggggtgaa gggcctgcat gcccggcgtc ggcggccagg cctcgaagga
     6181 gggggcgtcg ccgcccaccg cggcgaagtg gttgtcccag aggaagcgca gcgactcgtg
     6241 cccctgggcc accccggcga aggtgtgcgg ccccttggcc agggcggcgc gcgcctcggc
     6301 ggtgagcgat tcgtaccagg cggtgaagcc ggtgcgcacc agcaggatgt cgccagtctc
     6361 gaactgcaca ccctggtcgc cggccgcggc cagcagcccg gccacggtga tcgcctcgtg
     6421 ctcgccgggc gagtaggtga tgccgttggc ctcggcccag cggcggtagt cgatcagcac
     6481 gccgcgcccg gcgatgccgc gctcggccca gacgtggatg ctgttgcgcg tgcccgggcg
     6541 cccggtgatc tccgccggct gcacgcccat gaagaagcac tggtgctcgg cgttgccgta
     6601 gtgggtgaag ccgtcccagt ggctggagga ctgggggttg agcgcggtgt agaagtcgtc
     6661 gttgacgaag gggcgcttgt tgagcacggc gtgctcgctg cgctggcgga agaagagggg
     6721 cggctcgggc agctccatcg accagctgag gccgaagcgc cggccgctgc gcgccagccg
     6781 ggcggcccgc gccacgcgct cgggcgtcag caggttgagc atgcccagct cgtcgcgctc
     6841 gccccagaga ccccaggcgc agagcggcgg gttgccggga gtcaccggca gctcgtcgta
     6901 ggtcggcaat cggcttggct cgcgcccggt ctgcaccacc gctccctgct cctctcgcgc
     6961 tcgcggcttg ctcggctcca tcccggcgat tgtcggccgc agcggcggcg ccgtcaacgc
     7021 gggtccggcg ccagcgcgct agctctccag cggcaggccg atggcatgca ggcgtgcgcg
     7081 caggaattcc cgctgctcgt gctcggattt gaggcgcgcc cagttgtcga ggatggcctc
     7141 gacgcgcgcc aggtcggcgg gcggcagcgc ctggcgccgg atatcgatgg catcgcggat
     7201 cgcctgctgc tggaaggggg tgaggcgggc gagcttgcgc aagagattct cgcgctcgaa
     7261 gccccagcgc tgctctgggc cctcggcggc cggtgcggta tcgagcgcca ggcgcacctc
     7321 gtcgaccagc ggcagcgggg cacgttgggg caaggaccag agcgtgtggg tgaggaacgc
     7381 ggcctcctcc agggtaaagc gcgcgttccg gtcgatcccc gcgtcgggtc gcgcctccat
     7441 aggctcctcc tcatgacggt tgcggccgaa gatcgccggc cgatctcgcg cggcgacccc
     7501 gcagaacgcg caccgggcgg gccgggcagg atttgcctcg cggcggccga gcaggttatc
     7561 atggtgccgg acggtcaccg tctcgctgcc gatgtagctc agctggcaga gcagctgttt
     7621 cgtaaacagc aggtcgtcgg ttcgaatccg accatcggct cccccgaata actacaaatt
     7681 cgcgatcggc aacccgctca accacgagcg gacgtgttcg tcttcgctgg cgccatcctt
     7741 ccaggca
//
LOCUS       DASUBI010000759         8950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_537955, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000759 DASUBI010000000
VERSION     DASUBI010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537955"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2868)
                     /gene="uvrA"
                     /locus_tag="VFU72_14840"
     CDS             complement(<1..2868)
                     /gene="uvrA"
                     /locus_tag="VFU72_14840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5424819.1"
                     /translation="MASDKIVVRGAREHNLKNIDVEIPRDKLVVLTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDQIEGLSPAISIDQKSASRNPRSTVGT
                     VTEIYDHLRLLYARIGHAHCPNCGRPIAQQSVQQMAEQVLALPEGSRLLLLAPLIKDR
                     KGEHQGVLDEIRRAGFSRVRVDGEVREIEESFSLERYKRHTVEAVVDRLIVRHSDDEE
                     LHAARIRLIDSLETALRMGAGYARVQMIDGVELLFSEHYACAHCGLSFGELEPRSFSF
                     NSPHGACPECSGLGSRMEFDPDLIFPDRDRTLAGGAIAPWAKAGRDSTAWYDAVIAAL
                     ARKHGFAVDVPVRELPDKALDLILNSQGREKIRVEYKARSGRVRSFDITYEGVIPNLR
                     RRYDSTSSDYVRAEIEQYMAAQPCVACGGARLKPEARAVTVGERSIVEVSGMAIAEAL
                     AFVERLADGERGEPVLTNRETLIARQILKEIRERLSFLVDVGLDYLTLDRAAASLSGG
                     EAQRIRLATQIGSRLMGVLYILDEPSIGLHHRDNERLIRTLVRLRDLGNTLIVVEHDE
                     ETMRCADWIIDIGPGAGEHGGEVVAVGPLEAILAVPESVTGQYLSGARQIETPAERRR
                     GNGKWLTILGARENNLKGIDVSLPLGTFVCVTGVSGSGKSSLVADTLYPRLAQVLHRA
                     KARAGQHDAIQGIEHLDKVIEIDQSPIGRTPRSNPATYTGAFTPIRELFASMPEARAR
                     GYKPGRFSFNVKGGRCEACQGEGIIAIEMQFLPDVYVPCEVCHGKRYNEEALEVRYRD
                     KSIAEVLDLTVAEALEFFENIPAIRNKLQTLYDVGLGYIHLGQPATTLSGGEAQRIKL
                     ASELSRRATGRTLYILDEPTTGLHFADIERLLHVLQRLVDAGNSVIVIEHNLDVVKSV
                     DWIIDLGPEGGSAGGTIIAQGTPEEIAAVPRSYTGQYLAPLL"
     gene            3072..3902
                     /locus_tag="VFU72_14845"
     CDS             3072..3902
                     /locus_tag="VFU72_14845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424820.1"
                     /translation="MAFGPSQPGDPAAERVDVSLIIPAYNEAQRLPETLRQAVAFLGQ
                     QPFSWELIIADDGSEDATATLAAEAARQPGVRHLRLLHHGKAAAVRAGVRAARGRSII
                     FTDADLSTPITYVADTHRLLNDGWEVVIGTREAPAARRIGEPYYRHLMGRVFNWAVRL
                     LAVPGLRDTQCGFKGFRVEVARDLFRRARLYRDSQPVRGPLVTGFDVELLYLARRCGY
                     QLYELPVTWSHVAGSKVRPGLDSLLMLRDVLAVRRNALRGAYREPHSCEAGPLEPGQS
                     "
     gene            4091..5329
                     /locus_tag="VFU72_14850"
     CDS             4091..5329
                     /locus_tag="VFU72_14850"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424821.1"
                     /translation="MLARNRDFAALWGADVASTLGDRVHRVALAALIYELTGSLTQTG
                     LAFVAVMLPDLVLGLAAGVFVDRHDRRRVMVGCDLLRIPLVLALPLVAFAFLPAAYIL
                     LFVINSLTIVFRPAQQALIPVVVPTEELSAANAISQVSGNVSDIIGYPLGGLVVGVLG
                     AWLGTRTGLVTAFSIDSLTFLISALLVLTVRVHASPSPGARRRSITADLREGFAFLRK
                     CAVVRANTFVMLLGPLTLGAETPLLIGYAWGVLDRGSWAYGLLGFGISLGSILGGIVI
                     GTFAERLPGYIVVAGLAVMGLAIALVGAVANLWLAVLAMALCGVGNVMVLIPSVTLVQ
                     RQTPPELLGRIFSLRTTLIFGVLIASNAVGGWAGQVFGTRQTLIACGSLLLIAATLAS
                     FFPVTRTMRPSLPLEEPAFD"
     gene            5614..7884
                     /locus_tag="VFU72_14855"
     CDS             5614..7884
                     /locus_tag="VFU72_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5424822.1"
                     /translation="MRALLSTAGLARASARHPWRVLGIWLIVLVLAGVSASGLGKALT
                     TDANFTGTPESKKGAELIEQRLTGATAMTESIVVRSDTATVDDPAFKATVDHLAANLA
                     GLDGLIESAPNYYQLQAAGVPSAAQLISKDRHAMLIPVTFAGTHEAVTNRGDAYLDAV
                     HQAESADVQVLTVGDISANKEFNDIASKDLAKAEQLSLPITLVILVLVFGALVAAGVP
                     LVLAITSIVVAVGLTALVGRVMDLSFYVVNMITMIGLAVGIDYALFIVTRYREERRHG
                     LDTVEAITKAGATASKAVLFSGITVVFALSGMLLMPTTIFHSLGTGAVLVVIVAVAAM
                     LTLVPALLSLIGDKIDWPRRRRFDAAAVAAQERYDHETIHRGFWGTVARVVMTHPVPA
                     VVVAVALLGAVAIPFIDMHRGTSGIETLPPSDTKSAYTILNRDFSAGVLAPYEIVVDA
                     PKNADSEAAIGRLSDAIKSEGDFLPTVTVEWNQANDLALVKATLTQDSNSQAAYDTLT
                     TLRKQLVPNAFAGSPAKVYVTGATAFNADFFHLVDKYTPVVFVWVLGLSFLLLMLAFR
                     SLVVPAKAILMNLLSVGATYGLMVLIFQKGYLHNLFGFQQTPTIESWVPIMLFCVLFG
                     LSMDYHVFLLSRIREHFDETGANRTSVAVGLQATARLITGAALIMVVVFGGFAAGRLS
                     AFQQLGFGLAVAVLLDATIVRSVLVPAAMTLLGNANWYLPRWLEWLPDLRIEGGRPAA
                     PAPVPTPELAALGTDD"
     gene            7999..>8950
                     /locus_tag="VFU72_14860"
     CDS             7999..>8950
                     /locus_tag="VFU72_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5424823.1"
                     /translation="MPEFQLATDLRPTGDQPKAIDQLVQGLESGFKHQTLLGVTGSGK
                     TFSMANVIQCVQRPALVLAPNKTLAAQLYAEFKEFFPSNAVEYFVSYYDYYQPEAYVP
                     RTDTYIEKDAQINDEIDKLRHAATRALLTRPDTIIVASVSCIYGIGSPEDYGQSFVSL
                     RRGATVRRDRILRHLIDIQYDRNDLTLVRGTFRVRGDTIDIFPAYEEIAIRVEFWGDE
                     VERIVDIDPLTGEILAERNEIDIYPAKHFVTTQEKLLLAVKDIEAELEERLAELDAQG
                     KVLEAARLKQRTLYDLEMLQEAGYCSGIENYARHLARRKAG"
BASE COUNT         1363 a         3124 c         2936 g         1527 t
ORIGIN      
        1 gagaagagga gccaagtact ggcccgtgta cgaccgcgga acagcggcga tttcctccgg
       61 cgtgccctgg gcgatgatgg tgccgccggc ggagccgcct tccggaccga ggtcgatgat
      121 ccagtccacc gacttgacca cgtcgaggtt gtgctcgatg acgattacgc tgttgccggc
      181 atcgaccagc cgctgcagga cgtgcagcag tcgctcgatg tcggcaaagt gcaggcccgt
      241 ggtcggctcg tccaggatgt agagcgtgcg tccggtggcg cgccggctca gctccgaggc
      301 cagcttgata cgctgcgcct cgccgccgga gagcgtggtg gccggctggc ccaggtggat
      361 gtagcccagg ccgacgtcgt agagcgtctg cagcttgttg cggatggccg ggatgttctc
      421 gaagaactcc agcgcctcgg ccacggtcag gtcgagcacc tcggcgatgc tcttgtcgcg
      481 gtagcggacc tccagcgcct cctcgttgta gcgcttgccg tggcagacct cgcagggcac
      541 gtagacgtcg ggcaagaact gcatctcgat ggcgatgatc ccctcgccct ggcaggcctc
      601 gcagcggccg cccttgacgt tgaacgagaa gcggcccggc ttgtagccgc gcgcccgcgc
      661 ctcgggcatc gaggcgaaca actcgcggat cggggtgaag gcgccggtgt aggtggccgg
      721 gttcgagcgc ggcgtgcgcc cgatcgggct ctggtcgatc tcgatcacct tgtccagatg
      781 ctcgatgccc tggatggcgt catgctgccc ggcgcgtgcc ttggcgcggt gcagcacctg
      841 ggccagccgc gggtagagcg tatccgccac cagcgagctc ttgcccgagc cggacacgcc
      901 ggtgacgcag acgaacgtac cgagcggcag cgagacgtcg atccccttca ggttgttctc
      961 gcgcgccccg aggatcgtca gccacttacc gttgccgcgc cgacgctcgg ccggcgtctc
     1021 gatctgccgc gccccgctga ggtactgccc ggtgaccgac tccggcacgg ccaggatcgc
     1081 ttccagcggc ccgaccgcca ccacctcgcc gccgtgctcg ccggctcccg gcccgatgtc
     1141 gatgatccag tcggcgcagc gcatcgtctc ctcgtcgtgc tcgaccacga tcaaggtgtt
     1201 gcccaggtca cgcagacgga ccaaggtgcg gatcaagcgc tcgttgtcgc ggtggtgcag
     1261 gccgatgctc ggctcgtcca gaatgtagag cacccccatc agacggctgc cgatctgggt
     1321 cgccaggcga atgcgctgcg cctcgccgcc ggagagcgag gccgccgcgc ggtcgagcgt
     1381 caggtagtcg agcccgacat cgaccaggaa gctgagccgc tcgcggatct ccttcaggat
     1441 ctggcgcgcg atcagcgtct cgcggttggt cagcaccggc tcgccgcgct caccgtcggc
     1501 cagccgctcg acgaacgcca gcgcctcggc gatcgccatg ccggagacct cgacgatcga
     1561 gcgctcgccg acggtcacgg cgcgcgcctc gggcttcagg cgcgccccgc cgcaggccac
     1621 acagggctgg gccgccatgt actgctcgat ctcggcgcgc acgtagtcgg agctggtgct
     1681 gtcgtagcgc cggcgcaggt tggggatgac cccctcgtag gtgatgtcga aggagcgcac
     1741 acggccgctg cgcgccttgt actccacccg aatcttctcg cgcccctggc tgttgaggat
     1801 gagatcaagc gccttgtcgg gcaattcgcg caccggcacg tcgacggcga agccgtgctt
     1861 acgcgccagc gccgcgatga ccgcgtcgta ccaggcggtg ctgtcacgcc cggccttggc
     1921 ccagggcgcg atcgcgccgc cggccagcgt gcggtcgcga tcggggaaga tcaaatcggg
     1981 gtcgaactcc atgcgcgagc cgagcccgct gcactccggg caggcgccgt gcgggctgtt
     2041 gaacgagaag ctgcgcggct ccagctcgcc gaagctgagg ccgcagtggg cgcaggcgta
     2101 gtgctcggag aagagcagct ccacgccgtc gatcatctgc acccgagcgt agccggcgcc
     2161 catgcgtagc gccgtttcca gcgagtcgat caggcgaata cgcgccgcgt gcagctcctc
     2221 gtcgtcgctg tggcgcacga tcagacgatc gacgaccgcc tcgacggtat gccgcttata
     2281 gcgctccagg ctgaacgatt cctcgatctc gcgcacctcg ccgtcgacgc gcacccggct
     2341 gaagccggcg cgccggatct cgtcgagcac gccctggtgc tcgcccttgc gatccttgat
     2401 caggggcgcc agcagcagca ggcggctccc ctccggcaag gcgagcacct gctcggccat
     2461 ctgctgcacc gactgctggg cgatcggccg gccgcagttc ggacagtgcg cgtggccgat
     2521 gcgggcatag aggaggcgca ggtggtcgta gatctcggtc accgtgccca cggtcgagcg
     2581 cgggttgcgg ctggcgctct tctggtcgat cgagatggcc ggcgagaggc cttcgatctg
     2641 gtcgacgtcc ggcttctcca tcaaacccag gaactggcgc gcgtacgccg agagcgactc
     2701 gacgtagcgc cgttgcccct cggcgtagat ggtatcgaag gccaggctcg acttgcccga
     2761 gccggagagg ccggtcaaga ccaccagctt gtcgcggggg atctcgacgt cgatattctt
     2821 gaggttgtgc tctctggctc cgcggaccac gatcttgtcg ctcgccatgc cgtctccgtc
     2881 gaactcgaac aaacgatcgc aagtactcat tctacagcct tcggagccag agtgacaggg
     2941 ggacaagccg ctattaaggg aggtgaatcg cagcgaacga acgcgccttc gtcacgatcg
     3001 gtcaagctgg tacaatggaa cgtcgccagc gcgcgcccgc gccgccgcag cacgcaagaa
     3061 gggacccctc gttggccttc ggcccctccc aacccggcga cccggcggcc gagcgggtcg
     3121 acgtcagcct gatcatccct gcctacaacg aggcgcaacg cctcccggag accctgcgac
     3181 aggcggtcgc gttcctcggc cagcaacctt tcagttggga actgatcatc gccgacgatg
     3241 gcagcgagga cgccaccgcg acgctggccg ccgaggctgc caggcagccc ggcgtgcggc
     3301 acctgcgtct actgcatcat ggcaaggccg cggcggtgcg cgccggggtg cgggcggcgc
     3361 gcggccgctc gatcatcttc accgacgccg acctctcgac accgatcacc tatgtagcgg
     3421 acacacaccg gctgctgaac gacggctggg aagtggtcat cggcacgcgc gaggcaccgg
     3481 cggcccgccg catcggcgag ccctactatc gccacctgat ggggcgcgtc ttcaactggg
     3541 cggtgcggct cctggccgtg cccggtctgc gcgacaccca gtgcggcttc aagggttttc
     3601 gtgtcgaggt ggcgcgggac ctcttccgtc gcgcccggct ctaccgcgat agtcagccgg
     3661 tgcgcgggcc actcgtgacc ggcttcgacg tcgagctgct ctacctggcc cggcgctgtg
     3721 gctaccagct ctacgagctc cccgtcacct ggagccacgt ggccggcagc aaggtgcggc
     3781 cggggctgga cagcctcttg atgctgcgcg acgtgctggc ggtgcggcgc aacgccctgc
     3841 gcggcgctta ccgcgagccg cattcctgcg aggcgggtcc tctcgaacca ggacaatcgt
     3901 gaccgcccag cgggtcacaa atgtcctgcc ttacccttgt ttttcggccc gatctctact
     3961 ataaaatgag tgcgctcgcc gcgaacaatc gtcgtcttcc gcgcaccctt ggtggtgacc
     4021 attgacgcta cgcggcgagg gggaggggga agggtaggga atgcgcatcc cggccgcgga
     4081 tcgttttggc ttgctcgcgc gcaaccgcga tttcgcggcg ctgtggggcg ccgatgtcgc
     4141 ctccacgctc ggcgatcggg tccatcgcgt ggccttggcc gcgctgatct acgagctgac
     4201 cggctcgctg acccagaccg gcctcgcctt cgtggcggtc atgctccccg atctcgttct
     4261 cgggctggcc gccggggtct tcgtcgaccg ccacgatcgg cgccgggtga tggtcggctg
     4321 cgacctgctg cgcatcccgc tggtgctcgc cctgccgctg gtggccttcg ccttcttacc
     4381 ggcggcctac atcctcctct ttgtcattaa cagcttgacg atcgtcttcc gcccggcgca
     4441 gcaggcgctg atcccggtgg tggttcccac cgaagaactc tccgccgcca acgccatctc
     4501 ccaggtcagc ggcaacgtca gcgacatcat cggctacccg ctgggcgggc tggtcgtcgg
     4561 cgtactcggc gcctggctcg gtacccgcac gggcctggtc accgccttca gcatcgactc
     4621 gctcaccttt ctgatatcgg cgctgctggt cctgaccgtc cgcgtccacg catcccccag
     4681 cccaggcgcg cggcgccgct cgatcaccgc cgacctacgc gagggcttcg ccttcctgcg
     4741 caagtgcgcc gtggtgcgcg ccaacacctt cgtcatgctg ctcggcccgc tgacgctggg
     4801 cgccgagacg ccgctcctca tcggctacgc ctggggcgtg ctcgatcgcg gctcctgggc
     4861 ctacggcctg ctcggcttcg gcatcagcct ggggagcatc ctgggcggca tcgtcatcgg
     4921 cacctttgcc gagcgcctgc ccggctacat cgtggtagct gggctggcgg tgatggggct
     4981 ggcgatcgcg ctggtcggcg cggtggccaa tctctggctg gccgtgctcg cgatggcgct
     5041 ctgtggcgtg ggcaacgtca tggttctcat ccccagtgtc accctggtcc aacgccagac
     5101 accgccggag ctgctggggc gcatcttcag cctgcgcacc acgctcatct tcggcgtctt
     5161 gatcgcctcg aacgcggtcg gcggctgggc ggggcaggtc ttcggcacgc ggcagacctt
     5221 gatcgcctgt ggctcgctcc tcttgatcgc cgcgacactc gcctcattct tcccggtcac
     5281 gcgcaccatg cggcccagcc ttcccctcga agaacccgcc ttcgactgaa gacgggcgat
     5341 cgccgtccca ctgactgacc ggactcgcag agagaatccg gaacgtgcag agtcgttggg
     5401 ttgtgtaccc caatcccgtg agctgaggca ttcgtcgtgt gtgcggttcg acgtggggaa
     5461 ccgcctcccg gcctgtccca tcggacaggc cgccgccggt cggttgacca atgaccggcg
     5521 cgcgctccgt cgccatactg agcgaggaga gacacgcgcg ccgaccggct ggcgccgtgg
     5581 accgcggaca ttgttgagcg aaaggtaaac gccatgcgag cattgctctc taccgccggg
     5641 ctggcgcgcg ccagcgcgcg gcacccctgg cgtgtactgg gcatctggct catcgttctg
     5701 gtgctggcag gcgtctcggc gtccggcctg ggcaaggcgt tgacgaccga tgccaatttc
     5761 accggtacgc cagagtcgaa gaaaggcgcc gagctgatcg agcagcggct caccggcgcc
     5821 accgccatga ctgagtcgat cgtcgtgcgc tccgacaccg ccaccgtcga cgatcctgcc
     5881 ttcaaggcca ccgtcgatca cctggcggcc aacctcgccg gactcgacgg cctgatcgag
     5941 tcggcgccga actactatca actccaggcg gcgggcgtac cgagcgcggc gcagctcatc
     6001 tccaaggacc gccacgcgat gctgattccg gtcaccttcg cgggcacgca cgaagcggtc
     6061 accaaccgcg gtgacgcgta cctcgacgcg gtgcatcagg cggagagcgc cgacgtgcag
     6121 gtgctgaccg tgggcgacat cagcgccaac aaagagttca acgacatcgc ctcgaaggac
     6181 ctggcgaagg ccgagcagct ctcgctgccg atcaccctgg tcattttggt gctcgtcttc
     6241 ggcgcgctgg tggcggcggg cgtgcccctg gtgctggcga tcacctcgat cgtcgtggcc
     6301 gtaggtctga ccgcgctggt cggccgggtg atggacctgt cgttctacgt ggtcaacatg
     6361 atcaccatga tcggactggc ggtcggcatc gactacgccc tcttcatcgt cacccgctac
     6421 cgcgaagagc ggcgtcacgg gctcgacacc gtggaggcga tcacgaaggc gggcgcgacc
     6481 gccagcaagg ccgtgctctt ctccggcatc accgtcgtct tcgctctctc gggcatgctg
     6541 ctgatgccca ccaccatctt ccacagcctg ggcaccggcg cggtgctggt cgtcatcgtg
     6601 gcggtcgccg ccatgttgac cctggtgccg gcgctgctga gcctgatcgg cgacaagatc
     6661 gactggccac gccgccggcg cttcgatgcc gcggcggtgg ccgcgcagga gcgctacgac
     6721 cacgagacga tccatcgcgg cttctggggc acggtggcgc gcgtggtcat gacccacccg
     6781 gtgcccgccg tcgtggtggc cgtggcgttg ctgggtgcgg tcgccatccc cttcatcgac
     6841 atgcaccgcg gcacctccgg catcgagacg ctgccaccca gcgacaccaa gtcggcgtac
     6901 accatcctga atcgcgactt ctcggccggc gtgctggcgc cctatgagat cgtggtcgac
     6961 gcgcccaaga acgccgacag cgaggcagcc atcggccggc tgagcgatgc gatcaagagc
     7021 gagggtgact tcctgcctac cgtcaccgtc gagtggaatc aggccaacga cctggcgctg
     7081 gtcaaggcga cgctgacgca ggactcgaac tcgcaggcgg cctacgacac cctgaccacg
     7141 ctgcgcaagc agttggtccc gaacgccttc gcggggagcc cggccaaggt ctatgtcacc
     7201 ggcgccacgg ccttcaacgc cgacttcttc cacctggtcg acaagtacac gccggtggtg
     7261 ttcgtctggg tgctggggct gagcttcctg ctcctgatgc tggccttccg ctcgctggtc
     7321 gtgccggcca aggccatcct catgaatctc ctctcggtgg gcgcgaccta cggcctgatg
     7381 gtcttgatct tccagaaggg ctacctgcac aatctcttcg gcttccagca gacgccgacg
     7441 atcgagtcct gggtgccgat catgctcttc tgcgtcctct tcgggctcag catggactac
     7501 cacgtcttcc tgctcagtcg catccgcgag cacttcgacg agaccggagc caatcgcacc
     7561 tcggtggcgg tgggcctgca ggccaccgcg cggctgatca ccggcgcggc gctgatcatg
     7621 gtggtcgtct tcggcggctt cgccgccggc cggctgtcgg ccttccagca gctcggcttc
     7681 gggctggcgg tggccgtcct gctggacgcc accatcgtgc gctcggtgct ggtgccggcg
     7741 gcgatgacgc tgctgggcaa cgccaactgg tacctgccgc gctggctgga gtggctgccg
     7801 gacctgcgca tcgagggtgg ccggcccgcc gccccggcgc ccgttccgac tcccgagctg
     7861 gcggcgctcg gcaccgacga ctagccttcc aacagacctt cgcgggccgt ggcctcggtc
     7921 acggctcgtc ttcttctccc ccacttcggt atacttagag ccgcatcaga acaggcgtgc
     7981 gtaagacagg agcggccgat gccggagttc caactggcaa ccgatctgcg ccccaccggc
     8041 gatcaaccga aggccatcga ccagctcgtt cagggcctgg agtccggctt caagcaccag
     8101 acgctgctcg gcgtgacggg ctctggcaag acattttcca tggcgaacgt tattcaatgt
     8161 gtgcaaaggc ccgcattggt gctggcgccg aacaagacgc tggcggctca gctctacgcc
     8221 gagttcaagg agttcttccc cagcaacgcg gtcgagtact tcgtcagcta ctacgactac
     8281 taccagcccg aggcctacgt cccgcgcacg gatacctaca tcgagaagga cgcgcagatc
     8341 aacgacgaga tcgacaagct gcgccacgcc gccacgcgcg ccctcttgac gcggcccgac
     8401 acgatcatcg tcgccagcgt ctcctgcatc tacggcatcg gctcgccgga ggactacggg
     8461 cagtcgttcg tctccctgcg gcgcggcgcc acggtgcggc gtgaccgcat cctgcgccat
     8521 ctgatcgaca tccagtacga ccgcaacgac ctgacgctgg tacgcggcac gttccgcgtg
     8581 cgcggcgaca ccatcgacat cttccccgcc tacgaagaga tcgcgatccg agtcgagttc
     8641 tggggcgacg aggtcgagcg catcgtcgac atcgacccgc tgaccggcga gattctggcc
     8701 gagcgcaacg agatcgacat ctacccggcc aagcacttcg tgaccaccca ggagaagctg
     8761 ctgctggcgg tcaaggacat cgaggctgag ctggaggagc gcctggccga attggatgcc
     8821 cagggcaagg tgctggaggc ggcccggctc aagcagcgaa ccctctacga cctggagatg
     8881 ctgcaggagg ccggctactg ctcgggcatc gagaactacg cgcgccacct ggcgcggcgc
     8941 aaggcgggcg
//
LOCUS       DASUBI010000760         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_116964, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000760 DASUBI010000000
VERSION     DASUBI010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116964"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..933)
                     /locus_tag="VFU72_14865"
     CDS             complement(94..933)
                     /locus_tag="VFU72_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5424824.1"
                     /translation="MGNQWPEGARCVALFTCDFDGTGNEIGKKLAPAGINAAGGYSAR
                     RGVPRMLEIFDRRQIPATFFVPGYDAEQNPDTVRSIIAAGHEVAAHGYVHESFDVPED
                     EEERLLRKSHDILVNITGSAPLGWRSPGGKKSSITLAVLRDLGYIFDSSDKDYDQPYP
                     AVVSGEPSSAMVELPNNTSSLDDSPLYTTAAFSPAEVLARWQREFDAIYNDAGYFIVT
                     FHPRAGFGSGIPSRARVIDRLIAYIQTFPDVHFSRMADLARWCLDPAHGFMHPPARLG
                     GRR"
     gene            complement(1027..2073)
                     /locus_tag="VFU72_14870"
     CDS             complement(1027..2073)
                     /locus_tag="VFU72_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5424825.1"
                     /translation="MGRLVGLLVLILLLSVLAACATPSSIVTPTGVAIGTQPPPIATP
                     TPAAPSPAPSPISVTPTPVPAPLATPAPPPPTPVPTPMPTPEPTPPPVDAAWSGLQRD
                     LQAMVGRWEGQNAVSVTDLQSGRTISINGSRPQLAACTIKIPLLMIVAQDIEAGRYSA
                     DSVDSLVRSAMGPSNTWPARELLRTVGDGDIGAGIERVDDLMWSLGMNDSLLAHPPGY
                     DWEDYGYGDAENLLTTDDLNRLLGKLYRGEALSPWATGYVLWSMSLAPDWVNASLGGP
                     LPADVTLYHKFGQLYDPENTWNDAGIVVFERDGQEYAYAIAYLGSYGGDWRVAYDHAY
                     AVSARAWRYFDARY"
     gene            complement(2324..3565)
                     /locus_tag="VFU72_14875"
     CDS             complement(2324..3565)
                     /locus_tag="VFU72_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5424826.1"
                     /translation="MTSEPRVFISYQRADAEFARAARAQLMAHGITPWMDEYDIPVGA
                     YWPDAIDEALAASDVVIGLLSPDAVASRNVKNEWDWALQNDKRLLLVLVRPCVIPHRY
                     VSLNYIDATGPDPVGALTALVKELKPPRQGLAAKPPETRYALSGDLSIAYQVFGAGPV
                     DLVVTPGFVSNIDWIWDDPAMTRFYEGLAAFARVIAFDKRGTGLSDRTAGVPSLEQRI
                     DDLRAVMDAADAPQAVMMGISEGGSMAIQFAASHPERATALVLYGSFTSSSQARLPEE
                     ELRREEQTLLETWGRDASAMAARFAPSMAGDRRFVEWFAGYSRASASPGAVITLRRMN
                     RLIDLRDVLPAIATPTLVLHRVGDLDVSIEEGRYLAEHIPGARLIELPGQDHLPWVGD
                     QDAVVAAVRAFITEQMGPLAP"
     gene            4020..>5512
                     /locus_tag="VFU72_14880"
     CDS             4020..>5512
                     /locus_tag="VFU72_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5424827.1"
                     /translation="MLIARLDQITLSYGTREILRGVGLSLNDGEKIGLVGPNGAGKSS
                     LLKILAGLEPPQGGERSLRRGVRVAYLPQEYAGEVAPGAFDEVLLGRLELLACERELA
                     EIEARLADPALLDDPEGFGRALERQAALVEEYEAGGGPRLRNEASGLLTRLGLPEELH
                     ARPLAQLSGGQRKLVGLARCLVAQPQLLLLDEPDNHLDLAGKALLEEVLRDFKGAVIL
                     VSHDRYLLDDTVRRIIELDQGRLTEYEGNYSSYVVQRELALLKQQQDWVAQQKEIERL
                     EAAIARFKLWASMVVDERHIKQARNKQRQIDRMDKVERPVLERRQMGLRLRSAQRGGQ
                     KVIEARHLARFFGDEIVLLDASFTIMRGERVGLVGANGAGKSVLLRLLLGLDEPSEGE
                     VWIGPSIRLGYYAQGHETLNPADTPVAHMRRLKPMREDEAVGLLGKFLFGYRQSTGPI
                     ANLSGGEKSRLQLAWLMHGGANFLLLDEPTNHLDIAAIEVLEDALVR"
BASE COUNT          824 a         1786 c         2060 g          842 t
ORIGIN      
        1 cgggcaggac ggattgctcg acgctctcgc cgtcgaagtt gaccgtgatc gccacggcgc
       61 actgcgcgcc gttgggccag cgcggcgtcg cgctcatcgg cggcctccca atcgggctgg
      121 cggatgcatg aagccatgcg ccgggtcaag gcaccagcgc gccaggtcgg ccatgcggct
      181 gaagtggacg tcggggaagg tctggatata ggcgatcagc cggtcgatga cgcgcgcccg
      241 cgaggggatg ccgctgccga agccggcgcg cgggtggaag gtgacgatga agtagccggc
      301 gtcgttgtag atggcgtcga actcccgctg ccagcgcgcc agcacctcgg ccgggctgaa
      361 ggcggcggtg gtgtagagcg gcgagtcgtc caggctgctg gtgttgttcg gcagctcgac
      421 catggcgctg gagggctcgc cggagacgac ggctgggtag ggctggtcgt agtccttgtc
      481 gctggagtcg aagatgtagc ccaggtcgcg cagcacggcc agggtgatgc tgctcttctt
      541 gccgccgggg ctgcgccagc ccagcggcgc cgagccggtg atgttcacca ggatgtcgtg
      601 gctcttgcgc agcagccgct cctcttcgtc ttccggcacg tcgaagctct cgtggacgta
      661 gccgtgcgcc gccacctcgt gcccggccgc gatgatgctc cgcacggtgt ccgggttttg
      721 ctcggcgtcg tagcccggta cgaagaaggt ggccgggatc tggcggcggt cgaagatctc
      781 cagcatgcgc ggcacgccgc gccgcgccga gtagccgccg gccgcgttga tgccggccgg
      841 ggcgagcttc ttgccgatct cgttgccggt gccgtcgaag tcgcaggtga agagggcgac
      901 gcagcgcgcc ccctctggcc actggttgcc catggcgggt cgccgctcct tccgctgtcc
      961 gccccgcgtg gcccacgccg gtgctggggt gggcccattg tacgcgctcc tcccgcgctg
     1021 tcgagttcag taccgtgcgt cgaagtagcg ccaggcgcgg gcggagacgg cgtaggcgtg
     1081 gtcgtaggcg acgcgccagt cgccgccgta gctgccgagg taggcgatgg cgtaggcgta
     1141 ctcctggccg tcgcgctcga agacgacgat accggcgtcg ttccaggtgt tctcgggatc
     1201 gtagagctgg ccgaacttgt gatagagcgt cacgtccgcg gggagcggcc cgcccagcga
     1261 ggcgttcacc cagtccggcg ccagcgacat gctccagagc acgtagcctg tggcccaggg
     1321 cgacagcgcc tcgccccggt agagtttgcc gagtagccgg ttcaggtcgt cggtggtcag
     1381 caggttctcg gcgtcgccgt agccgtagtc ctcccagtcg tagccgggcg ggtgggccag
     1441 cagcgagtca ttcatcccca gcgaccacat caggtcgtcg acgcgctcga tgcccgcgcc
     1501 gatgtcgccg tccccgaccg tccgcaacag ctcgcgcgcc ggccaggtgt tgctcggccc
     1561 catggcggag cgcaccaggc tgtcgaccga gtcggccgag tagcgcccgg cctcgatgtc
     1621 ctgcgccacg atcatcagga gcgggatctt gatggtgcag gccgccagct ggggccgcga
     1681 gccgttgatc gagatggtcc ggccgctctg gagatcggtg acgctgacgg cgttctgtcc
     1741 ctcccagcgg ccgaccatcg cctggagatc gcgctggagc ccgctccagg cggcgtcgac
     1801 cggcggcgga gtgggctccg gcgtcggcat gggcgtcggc actggagttg gtggcggtgg
     1861 agccggcgtc gcgaggggcg ctggaaccgg cgtcggagtc acgctgattg ggctcggcgc
     1921 ggggcttggc gccgccggtg tgggggtcgc gataggcggc ggctgggtgc cgatcgcgac
     1981 gccggttggg gtgacgatgg agctgggggt ggcgcaagcc gccagcaccg agagcagcag
     2041 gatcaagacc aacagaccca caagacggcc catatccctg gctcctcgtc acctctccca
     2101 cgggaccgtg ctgtcccgct agctacaatt cgcgcgccgc ccggacgtga cgcgctctcc
     2161 ggacaggggc acgctgccca agcggacagg gcacacccgc cgcgaccgcg gcgcacgagc
     2221 ctgcccttta ttgaataccg gagcacgcaa cagaagcgtc gcggcggaca gcgcgatgtc
     2281 gcggaagcat gacgatttcg cgcgcgaggc ggcgccggcg atgctatggc gctagtgggc
     2341 ccatctgctc ggtgatgaag gcgcgcacag cggcgacgac cgcgtcctgg tcgccgaccc
     2401 aggggaggtg gtcctggccc ggcaattcga tcaacctggc gccggggatg tgctcggcca
     2461 ggtagcgtcc ctcctcgatc gacacgtcca gatcgcccac gcggtgcagc acgagtgtgg
     2521 gcgtagcgat cgccggcagc acgtcgcgga ggtcgatcag ccggttcatg cgccgcagcg
     2581 tgatgaccgc gccgggactg gcggaggcac gggagtaacc ggcgaaccac tcgacgaacc
     2641 ggcgatcgcc ggccatgctc ggcgcgaacc tggcggccat cgcgctggca tcgcgccccc
     2701 aggtctccag gagtgtttgc tcctcacgcc gcagctcctc ctccggcaac ctggcctgtg
     2761 acgagctggt gaaggagccg tagagcacca gcgcggtcgc acgctccggg tggctggcgg
     2821 cgaactgtat ggccatcgag ccgccttccg agatgcccat catgaccgcc tggggcgcgt
     2881 ccgcggcatc catcaccgcg cgcaggtcgt cgatgcgctg ctcgaggctc ggcacgcccg
     2941 ccgtgcggtc cgacagcccg gtgccgcgct tgtcgaacgc gatcacgcgc gcgaacgcgg
     3001 cgaggccctc gtagaagcgc gtcatggccg ggtcgtccca gatccagtcg atgttggaga
     3061 cgaagccggg cgtgacgacc agatcgaccg gcccggcgcc gaagacctga taggcgatgc
     3121 tcaggtcgcc gctcagggcg tagcgcgtct cgggcggctt ggcggccaaa ccctgacgcg
     3181 gcggcttgag ctccttcacc agcgctgtca gggccccgac cggatcgggc ccggtggcgt
     3241 cgatgtagtt gagcgagaca taacggtgcg ggatgacgca gggacgcacc aacaccagca
     3301 gcaggcgctt gtcgttctgc agcgcccagt cccactcgtt cttgacgttg cggctggcga
     3361 ccgcgtcggg gctgagcagg ccgatgacga catcgctggc ggccaacgcc tcgtcgatgg
     3421 cgtccggcca gtaggcgccg accgggatgt cgtactcgtc catccagggg gtgatgccgt
     3481 gcgccatgag ttgtgcgcgc gccgcgcggg cgaactcggc atcggcgcgc tggtaggaga
     3541 tgaaaacacg tggctcgctc gtcaccctgt cgcccacccc actgagtcga gttgccggtg
     3601 gcggggagca taccagatgc ccgcccggga tcgaggtccg gctccggccg tgcttcgctt
     3661 gtcagcacgc cgcctgcctg ctaggatgag gcgtgctcgt tccgccggga cgggcgatcg
     3721 tgtgtagcgg gagttgtcgt cgagccatgt ggcgcagcgc ggacggacgc ggccgacggg
     3781 gacagttgag gcagaagggc tccagcgcgg gccgtcaatt gccgtactgt gcccattttt
     3841 cggtgggtcc gcggtgtgac ggtgagcgag ggcgacggcc agcctagcaa cgagcaaaca
     3901 gccgcaccag gacaggaagc atcgaaacgt cgtgggccag tcgagcggcc cgcggcgttt
     3961 ttcgtatcgg agcactccgt acgatccgcg gcccggctgg ctccgtggag gaggaagcca
     4021 tgctgatcgc gcgactcgac cagatcacgc tgagctacgg cacgcgggag atcctgcgcg
     4081 gcgtcgggct gagcctgaac gacggcgaga agatcgggct ggtcgggccg aacggcgccg
     4141 gcaagtcgag cctgctgaag atcttggccg ggctggagcc gccgcagggc ggcgagcgca
     4201 gcctgcggcg cggggtgcgc gtcgcgtacc tgccgcagga gtacgccggc gaggtcgcgc
     4261 cgggcgcctt cgacgaggtg ctgctggggc ggctcgaact gctggcgtgc gagcgcgagc
     4321 tggccgagat cgaagcgcgc ctcgcagacc cggcgctgct ggacgatccc gagggcttcg
     4381 gccgcgcgct ggagcggcag gcggcgctgg tggaggagta cgaggccggc ggcggcccgc
     4441 gcctgcgcaa cgaagcgagc ggcctcctga cccgtctggg gttgccggag gagctgcacg
     4501 cgcgcccgct ggcgcagctc agcggggggc agcgcaagct ggtggggctg gcccgctgcc
     4561 tggtggctca gccgcaactg ctgctgctcg acgagccgga caatcatctc gatctggccg
     4621 gcaaggcgct gctggaggag gtgctgcgcg acttcaaggg cgcggtcatc ctggtctcgc
     4681 acgaccgcta tctgctggac gataccgtgc gccgcatcat cgagctggac caggggcggc
     4741 tgaccgagta cgagggcaac tactccagct atgtggtgca gcgcgagctg gcgctgctca
     4801 agcagcagca ggactgggtg gcgcagcaga aggagatcga gcggttggag gcggccatcg
     4861 cgcgcttcaa actctgggcc agcatggtgg tggacgagcg ccatatcaag caggcgcgca
     4921 acaagcagcg gcagatcgac cgcatggaca aggtcgagcg tccggtgctg gagcggcgcc
     4981 agatgggtct gagattgcgc tctgcccagc gcggcgggca gaaggtgatc gaggcgcggc
     5041 acctggcgcg cttcttcggc gacgagatcg tgctgctgga cgccagcttc accatcatgc
     5101 gcggcgagcg ggtcgggctg gtgggcgcca acggcgcggg caagtcggtc ctgctgcgac
     5161 tcttgctggg actggacgag ccgagcgagg gcgaggtctg gatcggtccc tcgatccggc
     5221 tgggctacta cgcgcagggg cacgagacgc tgaacccggc tgacacgccg gtggcgcaca
     5281 tgcggcggct gaagccgatg cgcgaggacg aggcggtggg gctcttgggc aaattcctct
     5341 ttggctaccg gcagtcgacc ggcccgatcg ccaatctcag cggcggcgag aagagccggc
     5401 tgcagctcgc ctggctgatg cacggcggcg ccaacttcct gctgctggac gagccgacca
     5461 atcatctgga tatcgccgcc atcgaggtgc tagaggacgc gctcgtccgc tt
//
LOCUS       DASUBI010000761         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_633280, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000761 DASUBI010000000
VERSION     DASUBI010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633280"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..445)
                     /locus_tag="VFU72_14885"
     CDS             complement(107..445)
                     /locus_tag="VFU72_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5424828.1"
                     /translation="MMTRVIERERSAGAVDEMQSFAERGIDAAALSGLLGRARWLAAL
                     TPGERDVLTLLAGRRSDAQIAETLALAPLVVESSLDSLYRKLGVRTRDALAAYARYQG
                     LGAGSQATGV"
     gene            677..2944
                     /locus_tag="VFU72_14890"
     CDS             677..2944
                     /locus_tag="VFU72_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5424829.1"
                     /translation="MTATSIIGQALPRLDSAEKVRGQTRYTADIHLPGMLYARFVTSY
                     QAHARITDIDTSAARAVEGVVDVVSGRDLMPDGPEPDMRARAMLARDRVLFYGQPVVA
                     VLAASEAAAEEAAGLVEVRYEPLGVNVDPIAAMRPDAPPIRAQEPGAESAEAGMHATV
                     TGGEHVSTYGLGPNVINALAFRRGNVEHGFNEADVVVERTYKTHFVHQGYIEPHASVA
                     VPDPLGRVTVYTATQGQFYARNTTADVLGIDQTEVTLVPMEVGGGFGGKTVLLEPLAA
                     ALALRVGRPVKIVLTRMEEFLHATPAPGSVIEIKTGAKKDGTITAIQARAVFDSGCYG
                     GAPVNVALMAIGGYYRCANLDLEGYEVITNKPGVGAYRAPGAPQATFVIEQQVDEMAR
                     QLGWDPLEFRLHSCSLEGDPQANDTPWPPLAMKQILETMRDHPLLKQKPTGKNEGVGY
                     AIGGWPGGVEPCAANLRVNTDGTLTLQLGSVDISGTNTVMAMIAAEVFKMPLEKVKIV
                     TADTGTAPYTGMSGGSKITYTVGNAVRAAAQDARNQVLAIAATELEAAPDDLELENGK
                     VWVKGSPDRSMPLGRVAHLSMTFGGKYEPVYGIGKSAVQDRAPGFSGQIAHVRVDPDT
                     GEVEMLKLVVVQDVGTALNPALIRGQMMGGATQGVGWALHEGMIYDDNGIPLNPSLMD
                     YSLPQSNQVPELETILVEMASKAGPYGAKGVGEPPVIPTAAAIANAVADASGARVTTL
                     PITSQRVSEALQQKG"
     gene            complement(3065..>3724)
                     /locus_tag="VFU72_14895"
     CDS             complement(3065..>3724)
                     /locus_tag="VFU72_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424830.1"
                     /translation="HTVYPVTLTGLGERAHLGGPWVSLDTHIDDVVNLLRYEDLHDVM
                     LAGHSYAGTVIAGVADRAPERIARLVYVDTWPLPDGVAQHDANSDERKRAEEEQVATE
                     GEGWRLPLPSWEELDQGNDLRGLGEAERALMRERAVAHPFGSLTQPLRLTNPACEGLP
                     RTAIWTSMTTDEVREMADAYPKFLGELARPDWQFVELPTGHWPMFSRPHDLAELLASL
                     A"
BASE COUNT          583 a         1355 c         1281 g          505 t
ORIGIN      
        1 atcctgagct tgatcctgag cctgccgaag gaaggatccg tcctcgatgg cgctcaacct
       61 agtcgaagag cactcccgtg agggcggcca gtccacctgg gcggactcag acgccggtag
      121 cctggctgcc ggcgccgagg ccctggtagc gcgcgtaggc ggccagcgcg tcgcgggtgc
      181 gcacgccgag cttgcggtag aggctgtcga ggctgctctc gacgaccaat ggcgccagcg
      241 ccagggtctc ggcgatctgc gcatcgctgc gacggcccgc caacagcgtc agcacgtcgc
      301 gctcgcccgg cgtcagcgcc gccagccagc gcgcccgccc gagcagcccg ctcaacgccg
      361 ccgcgtcgat ccctcgctcc gcgaagctct gcatctcgtc caccgccccg gcactccgtt
      421 cccgctcgat gacgcgcgtc atcatgacga tcgtatccgg ccatctagga cacacccaga
      481 gccgaacgtc ctgcccgcgc ccagacagcc ggaccgcggc tctcaggaac cattgtcgcg
      541 cccggcggtt gtcgtgcggt tgagccgatg agcccggccc ccttgacgct ggcgcggcgg
      601 acgccgaaga tacgagccgg gtctcccgcg ccagcggggc agatccatca catgactggc
      661 caatcaagga gggacgatga ccgccacttc catcatcggg caagcgcttc cccggctcga
      721 ttcggccgag aaggtccggg gacagacgcg ctacaccgcc gacatccact tgccgggcat
      781 gctctacgcc cgcttcgtga cctcgtacca ggcgcacgcc cgcatcaccg acatcgacac
      841 ctcggccgcc cgcgccgtgg agggggtcgt cgacgtcgtc tccggccgcg acctgatgcc
      901 ggacggcccg gagccggaca tgcgtgcccg cgccatgctg gcgcgcgatc gcgtcctctt
      961 ctacggccag ccggtggtcg ccgtgctggc ggccagcgag gccgccgccg aagaggccgc
     1021 cgggctggtc gaggtacggt acgagccgct gggggtcaac gtcgacccga tcgcggccat
     1081 gcgccccgac gcgccgccga tccgcgccca ggagccgggg gcggagtcgg ccgaggccgg
     1141 catgcacgcc accgtgactg gcggcgagca cgtcagcacc tacgggctcg gccccaatgt
     1201 gatcaacgcg ctcgccttcc ggcgcggcaa cgtcgagcac ggcttcaacg aggccgacgt
     1261 ggtcgtcgag cgcacctaca agacgcattt cgtgcaccag ggctacatcg agccgcacgc
     1321 cagcgtggcg gtgcccgatc cgctcgggcg cgtcaccgtc tacaccgcca cccaggggca
     1381 gttctacgcc cgcaacacga ctgccgacgt gctcggcatc gaccagaccg aggtcacgct
     1441 ggtgccgatg gaggtcggcg gcggcttcgg cggcaagacg gtactgctgg agccgctggc
     1501 ggcggcgctg gcgctacgcg tcggccgccc ggtcaagatc gtgctgacgc gcatggagga
     1561 gttcctgcac gccaccccgg cccccggctc ggtgatcgag atcaagaccg gcgccaagaa
     1621 ggacggcacg atcaccgcca tccaggcgcg cgcggtcttc gactccggct gctacggcgg
     1681 cgccccggtc aacgtggcgc tgatggcgat cggcggctac taccgctgcg ccaacctcga
     1741 tctggaaggc tacgaggtta tcaccaacaa gccgggcgtc ggcgcctacc gcgcccccgg
     1801 cgccccgcag gccaccttcg tgatcgagca gcaggtcgat gagatggccc gccagctcgg
     1861 ctgggacccg ctcgagttcc gcctgcacag ttgctcactg gagggcgacc cgcaggccaa
     1921 tgacaccccc tggccgccgc tggcgatgaa gcagatcctg gagacgatgc gcgatcaccc
     1981 gctgctgaag cagaagccga ccggcaagaa cgagggcgtc ggctatgcca tcggcggctg
     2041 gccgggcggc gtcgagccct gcgcggccaa cctgcgcgtc aacaccgacg gcaccctgac
     2101 gctgcagctc ggctcggtcg atatcagcgg caccaacacg gtgatggcga tgatcgccgc
     2161 cgaggtcttc aagatgccgc tggagaaggt gaagatcgtc accgccgaca ccggcacggc
     2221 gccctacacc ggcatgagcg gcggcagcaa gatcacctac accgtgggca atgccgtgcg
     2281 cgcggcggcg caggacgccc gcaaccaggt gctggcgatc gccgccaccg agctggaggc
     2341 ggcgcccgac gacctggagc tggagaacgg caaggtctgg gtcaagggct cgcccgaccg
     2401 ctcgatgccg ctcggccggg tggcgcacct gagcatgacc ttcggcggca agtacgagcc
     2461 ggtctacggc atcggcaagt cggcggtaca ggaccgcgcc cccggcttct cgggccagat
     2521 cgcgcacgtg cgcgtcgacc ccgacaccgg cgaggtcgag atgctgaagc tggtggtggt
     2581 gcaggacgtt ggcacggcgc tcaatccggc gctgatccgc ggccagatga tgggcggggc
     2641 gacgcagggt gtcggctggg cgctgcacga gggcatgatc tacgacgaca acggcatccc
     2701 gctgaacccc agcctgatgg actacagcct gccgcagtcg aaccaggtgc cggagctgga
     2761 gacgatcctg gtcgaaatgg cctccaaggc cggcccgtac ggcgccaagg gcgtgggcga
     2821 gccgccggtc attccgaccg cggccgccat cgccaacgcg gtggccgacg cctccggcgc
     2881 gcgcgtcacc acgctgccga tcacgtccca gcgcgtctcc gaggcgctgc agcagaaggg
     2941 gtaggcggac tctcacccca acggggagcc ctcaccccaa cccctttccc gatgcgcggg
     3001 agaggggcga gatcgcgact gtaggggcgg gtttgaaacc cgcccttctt cgttcgggcc
     3061 gcggctacgc caggctggcc agcagctcgg ccaggtcatg cgggcgggag aacatgggcc
     3121 agtggccggt gggcagctcg acgaactgcc agtccggcct ggccagctcg ccgaggaact
     3181 tcggataggc gtcggccatc tcgcgcacct cgtcggtcgt catgctcgtc cagatcgccg
     3241 tgcgtggcag cccttcgcag gccgggttgg tcagccgcag cggctgcgtc agcgagccga
     3301 aggggtgcgc gacggcgcgc tcgcgcatga gtgcccgctc ggcctcgccc agcccgcgca
     3361 ggtcgttgcc ctggtccagc tcctcccagg agggcagcgg cagccgccaa ccctcgccct
     3421 ccgtcgcgac ctgctcctcc tccgcccgct tgcgctcatc cgagttggcg tcgtgctggg
     3481 ccacgccgtc ggggagcggc caggtgtcga cgtagaccag ccgcgcgatg cgctccggcg
     3541 cccgatcggc gaccccggcg atgacggtgc ccgcgtagct gtgcccggcc agcatcacgt
     3601 cgtgcaggtc ctcgtagcgc aacaggttga ccacgtcgtc gatgtgggtg tcgaggctca
     3661 cccacggccc gccgagatgg gcgcgctcgc ccagcccggt gagcgtcacc gggtagacgg
     3721 tgtg
//
LOCUS       DASUBI010000762         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_973732, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000762 DASUBI010000000
VERSION     DASUBI010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973732"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFU72_14900"
     CDS             <1..301
                     /locus_tag="VFU72_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424831.1"
                     /translation="WGGMLLAHVSAATAARYGVAIQAEAIVDQAHRIILILEAAERDQ
                     IERFMRFYQRFGPVAISLASTSEAAVARGGCAEPEAASGSRNLVDYGSSGSGGSR"
     gene            complement(268..771)
                     /locus_tag="VFU72_14905"
     CDS             complement(268..771)
                     /locus_tag="VFU72_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424832.1"
                     /translation="MSEEREQEREQERERAGGGGQLSAAARRERLQELAAALGERAVS
                     EGERLILESGGAVALYVTVEPDGAFRLTWWQTATAPDGHPRWREAAATLTQPGEAIYT
                     SALGGGFLVELERRAASIDEARRLAGETAPVERVAALAAAFPDALGQLPPEPVPAYLL
                     PPEPEEP"
     gene            951..1874
                     /locus_tag="VFU72_14910"
     CDS             951..1874
                     /locus_tag="VFU72_14910"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5424833.1"
                     /translation="MAISQQYPGVFAAMSTPMTAAGEPDPAGIRNVVDYLFASGVSGL
                     SLLGSTGESPLLSIAQRQLVLETALEAANGRGTIYTGAASTVVADVITSLKTASRGAA
                     GALVPPPYYYPLGNDGVIGFYQHVAEASPVPIIMYNIPRMTKIQITPEAVSTLIEHPN
                     IMGLKDSSGDFGYFCSIARIGKEQGNGGFTLYTGSDAMLAVSLYAGGQGVIGGTVNVV
                     PDVEARLFKAFQQGDHEQAVALQGRIAKATAATGVGTFPAGVKGQYALKGLCGPHMAP
                     PIPALTEQQMNELRGRLVEAGVQLNVAVKGA"
     gene            1877..>2250
                     /locus_tag="VFU72_14915"
     CDS             1877..>2250
                     /locus_tag="VFU72_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5424834.1"
                     /translation="MAEAWDQYLSEHAERQLDELKEWLRIPSVSALPEHQADVRQSAE
                     WIADKLRQAGVPEVTLLPTERNPVVFGHWHVDDNQPTALIYGHYDVQPPDPLDLWESP
                     PFEPTVREGKLFARGAADDTGNA"
BASE COUNT          336 a          836 c          765 g          313 t
ORIGIN      
        1 gtggggcggc atgctgctgg cccacgtctc ggccgccacc gccgcgcgct acggcgtggc
       61 catccaggcc gaggcgatcg tcgatcaggc acaccggatc atcctgatcc tggaggcagc
      121 cgagcgcgac cagatcgagc gcttcatgcg cttctaccag cgtttcggcc ccgtcgcgat
      181 ctcgctcgcc tcgaccagcg aggcagccgt ggcgcgcggc ggctgcgccg agccggaggc
      241 tgcctccggc tcgcggaacc tcgtcgatta cggctcctca ggctcgggtg gcagcaggta
      301 ggcgggcacc ggctcgggtg ggagctggcc cagcgcgtcg gggaaggcgg cggccagcgc
      361 cgcgacgcgc tcgaccggcg ccgtctcgcc cgccagcctg cgcgcctcgt ctatgctcgc
      421 cgcgcggcgc tccaactcaa ccaggaagcc gccgccgagc gccgaggtgt agatcgcctc
      481 gccgggctgc gtcagggtgg cggcggcctc gcgccagcgc gggtgaccgt cgggcgcggt
      541 ggccgtctgc caccaggtca ggcggaaggc gccgtccggc tcgaccgtga cgtagagcgc
      601 gaccgcgccg ccgctctcca ggatcagccg ctcgccctcg ctgaccgcgc gctcgcccag
      661 cgccgctgcc agctcttgca accgctcgcg ccgcgcggcc gccgagagct gcccgccgcc
      721 accagctcgc tcgcgctcct gctcgcgctc ctgctcgcgc tcttccgaca tggtcccttc
      781 ctcctcgtac tcgcttcgtc caccgcttcc agctcgccgc cactatgcta cgatgcccgc
      841 gaacggctgt aatcacgttc agtgcgcctg aacgctcgct ctggacggcc cgccgctcgc
      901 gtggcgcggc cagtgcgcga ggatcgaagg actgaaagga agcagacccg atggcaatct
      961 cccagcaata ccctggcgtc ttcgcggcga tgtccacccc gatgaccgcc gccggtgagc
     1021 cggaccccgc cggtatccgc aacgtggtcg actatctctt cgccagcggc gtgagcgggc
     1081 tctcgctgct cggctcgacc ggcgagtcgc cgctgctcag catcgcccag cgccagctgg
     1141 tgctggagac agcgctggag gcggccaacg ggcgcggcac gatctacacc ggcgcggcca
     1201 gcacggtcgt tgccgacgtg atcacgagtc tgaagactgc ctcacggggc gcggcgggcg
     1261 cgctggtgcc gccgccctac tactacccgc tgggcaacga cggcgtgatc ggcttctacc
     1321 agcacgtggc cgaggccagc ccggtgccga tcatcatgta caacatcccg cgcatgacca
     1381 agatccagat cacacccgag gcggtctcga ccctgatcga gcaccccaac atcatgggcc
     1441 tgaaggactc cagcggcgac ttcggctact tctgcagcat cgcccgcatc ggcaaggagc
     1501 aggggaacgg cggcttcacc ctctacaccg gctccgacgc catgctggcc gtctcgctct
     1561 acgccggcgg tcagggcgtg atcggcggca cggtcaacgt cgtgccggat gtcgaagcgc
     1621 ggctcttcaa agccttccag cagggcgacc acgagcaggc cgtcgcgctg cagggacgca
     1681 tcgccaaagc gaccgcggcc accggcgtcg gcaccttccc ggccggggtc aaggggcagt
     1741 acgcgctcaa ggggctgtgc ggcccgcaca tggcgccgcc gatcccggcg ttgacggagc
     1801 agcagatgaa tgagctgcgc gggcggctgg tcgaggcggg cgtgcagctc aacgtcgcgg
     1861 tgaagggagc ctgagcgtgg ccgaagcctg ggaccagtat ctgagcgagc acgccgagcg
     1921 ccagctcgac gagctgaagg agtggctgcg catccccagc gtcagcgcgc tgcccgagca
     1981 tcaggcggac gtgcgccaga gcgccgagtg gatcgccgac aagctgcgcc aggcgggcgt
     2041 gcccgaggtg acgctcttgc cgaccgagcg caacccggtc gtcttcggcc actggcacgt
     2101 ggacgacaac cagccgaccg cgctgatcta cggccactac gacgtgcagc cgcccgaccc
     2161 gctcgatctc tgggagtcgc ctcccttcga accgacggtg cgcgagggca agctcttcgc
     2221 ccgcggcgcg gccgacgaca cggggaacgc
//
LOCUS       DASUBI010000763         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_708075, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000763 DASUBI010000000
VERSION     DASUBI010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708075"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFU72_14920"
     CDS             complement(<1..454)
                     /locus_tag="VFU72_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5424835.1"
                     /translation="MSETMQGKRRRFTVDALFSDTTPRALGVSDLPNAKEIQLERIVP
                     DPQQPRRSFDEERLEELAASIRREGVLQPIAVRYDRDGERYVILHGERRWRAARLAGL
                     HAIPAVVREVAEERRLLQQLMENVVREDLNAIDRAAALRALKTQMDDAP"
     gene            complement(447..1232)
                     /locus_tag="VFU72_14925"
     CDS             complement(447..1232)
                     /locus_tag="VFU72_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424836.1"
                     /translation="MPPIVAFFNQKGGTAKTTSTLNSGAALVERGCRVLAIDLDPQAS
                     LTMALGVDVRTLERSVYDLLIEEAVPLSAVTRATGVAGLDLVPSHPDLAAAELELLNV
                     LERERRLDDKLRDADLPYDYVLIDSPPALNILSINILVAAGFLVIPIEPHPLSLMVLR
                     RLFETVERVRRLNPRLQVVGFLPTKVHHSSRLAADMILTLQEEFPHLRLLPSVPLSVK
                     GAESIAEHSSILQYLPRSALSAAYRQVAEELERTVGSGSVANV"
     gene            1384..2223
                     /locus_tag="VFU72_14930"
     CDS             1384..2223
                     /locus_tag="VFU72_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU5424837.1"
                     /translation="MQARRPRTLLFSNLLIVLGIMLLGATVNAASAGGGLGAFRSVAP
                     PVSVALAAPAALTSAPTPTPLATTAPSLANAATPAATAPRTPAPAATPAGTPPAALPP
                     IIQPSASAAAPVSAAPTPAPTPTPRPKQPPATHISIPAVGIDTKVVEVGYDLVTIEGQ
                     QVIQWQVADYAAGHNNLSANPGEGGNIVISGHDDWRGEVFRTLDKVKLGDQITLTTPK
                     GPYRYAVTEIEYRKEVGVPLSERLATGMFLAPMPEERVTLVTCWPYGVDDHRLIVIAK
                     PVG"
     gene            2315..>3422
                     /locus_tag="VFU72_14935"
     CDS             2315..>3422
                     /locus_tag="VFU72_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5424838.1"
                     /translation="MSFQLPHLLAGRYELTQRLGEGSFSETYLATDTALGRRVAVKVL
                     RASFAGDPRFVARFEREARAAAAVASPNVVDVYDYGRQNDTLYIVMEWVDGIDLKQLI
                     RQRAPLPVPEAIALIDGILNGLQAIHRAGVVHRDIKPQNVLLAADGTPKLGDFGIARS
                     PVDAGLTDTGMALGTAAYMAPEQATGAPPTPSADVYAAGVILFELLTGRLPFPGENPV
                     QVMYQHVNTPPPNPRAFNPAIPPALGAVTLRALAKDPATRFADAQQMQSAIDGLPAAD
                     AATAMLPKADPGPAEQTRAMRAAAGGGAGNALPPPPPPPYRRVLQSSGPDWLPFLLAG
                     LLVVLVIGGVIFLASHNNGSPATPTVPVAVASPTA"
BASE COUNT          507 a         1292 c         1126 g          497 t
ORIGIN      
        1 agggcgcgtc gtccatctgc gtcttgaggg cccgcagcgc cgctgcccgg tcgatggcgt
       61 tcaggtcctc gcggaccacg ttctccatca actgctgcag gagccgccgc tcctcggcca
      121 cctcgcgcac caccgctggg atggcgtgca gcccggccag ccgcgcggcg cgccagcggc
      181 gctcgccgtg caggatcacg tagcgctcgc cgtcgcggtc gtaacgcacc gcgatcggct
      241 gcaggacccc ctcgcgccgg atcgaggcgg ccagctcctc cagccgctcc tcgtcgaagc
      301 tgcgccgcgg ctgctgggga tcgggcacga tgcgctcgag ctggatctct ttggcgttgg
      361 gcaggtcgct gacgccgagc gcgcgcgggg tggtgtcgct gaagagggcg tcgacggtga
      421 agcgccgccg cttcccctgc atcgtctcag acattggcca cactcccgct ccccaccgtg
      481 cgctccaact cctccgccac ctgccggtag gccgccgaga gcgcggagcg cggcaggtac
      541 tgcaagatcg agctgtgctc ggcgatcgac tcggccccct tgaccgagag tggcacgctg
      601 ggcaagagcc gcaggtgcgg aaactcctcc tgcaacgtca ggatcatgtc ggcggccagg
      661 cgcgaagagt gatggacctt ggtcggcagg aagccgacca cctgcaggcg cgggttgagc
      721 cggcgcacgc gctcgaccgt ctcgaagagc cggcgcagca ccatcagcga gagcgggtgc
      781 ggctcgattg ggatcaccag gaagccggcc gccaccagga tgttgatgct caggatgttg
      841 agcgccgggg ggctgtcgat caggacgtag tcgtaaggaa gatcggcgtc gcgcagcttg
      901 tcgtccaggc gtcgctcgcg ctccagcaca ttcagcagct ccagttcggc cgccgccaga
      961 tccgggtggc tcggcaccag gtcgagcccc gccacgccgg tggcgcgcgt caccgcggac
     1021 agcggcacgg cctcctcgat caggaggtcg tagactgaac gttcgagcgt ccggacatcg
     1081 acaccgaggg ccatcgtcag gctggcctgg ggatcgaggt cgatggccag cacgcgacag
     1141 ccgcgttcaa ccagcgccgc gccgctgttg agcgtgctgg tcgtcttggc cgttccgccc
     1201 ttttgattga aaaaggcgac gatcggcggc atgatggtcc cccctgcgtt gtgtacgtac
     1261 attttgtccc gatgatagca gatcgcgccc gcggcgtcta gcggccggtg ccgctcggtc
     1321 accgctgtac ggacaaaccg cgcccgccgc tcagtcgtta tcatgctggc ggatggaggt
     1381 gtcatgcaag ccaggcggcc gcggacgctg ctcttcagca atcttttgat cgtgctcggg
     1441 atcatgctcc tgggcgccac ggtcaacgcc gcgtcggcgg gcggtggcct gggcgccttc
     1501 cgctcggtcg cgccaccggt cagcgtggcg ctggccgccc ccgccgcgct cacgtccgcg
     1561 cccacaccca ctccgctcgc caccaccgct cccagcctgg ccaatgccgc cacgccggca
     1621 gctacggcac ctcggacgcc ggctcccgcc gcgacccccg ccggcacacc gccggccgcg
     1681 ctaccgccga tcatccagcc ctcggcgtcg gctgccgcgc cggtcagtgc cgcgccgacg
     1741 ccggcgccca cgccgacgcc gcggccgaag cagccaccgg cgacccatat ctcgatcccg
     1801 gccgtcggta tcgacaccaa agtggtcgag gtcggctacg atctggtcac gatcgagggc
     1861 caacaggtca tccagtggca ggtggccgac tacgccgccg ggcacaacaa tctctccgcc
     1921 aaccccggcg agggtggcaa catcgtcatc agcggccacg acgactggcg cggcgaggtc
     1981 ttccgcaccc tggacaaagt caagctgggc gaccagatca ccctgaccac gccgaagggc
     2041 ccgtaccgct acgcggtgac cgagatcgag taccgtaagg aggtcggcgt gcccctctcc
     2101 gagcggctgg ccaccggcat gttcctggcg ccgatgcccg aggaacgcgt caccctggtc
     2161 acctgctggc cgtatggtgt ggacgatcac cggctgatcg tgatcgccaa gccggtcggt
     2221 tagcgcggcc cgcaccgtcg cccgggcaga gccaaacccg tacaatgccg gccacagcga
     2281 ccgggtcgct gtaccatgcc ggattggaga cgacgtgtcc tttcaattgc cccacctgct
     2341 ggcgggccgc tacgagctga cccagcggct cggcgagggg agtttttcgg agacctacct
     2401 ggcgaccgac accgcgctgg ggcggcgcgt cgccgtcaag gtgctgcgcg ccagcttcgc
     2461 gggcgacccg cgcttcgtgg cgcgtttcga gcgcgaggcg cgcgccgccg ctgccgtcgc
     2521 cagccccaac gtggtcgacg tctacgacta tgggcgccag aacgacaccc tctacatcgt
     2581 gatggagtgg gtcgacggca tcgacctcaa gcaactgatc cgccagcgcg cccccctgcc
     2641 cgtgcccgag gcgatcgccc tgatcgacgg catcctcaac ggcctgcaag ccatccatcg
     2701 cgccggggtg gtgcatcgcg acatcaagcc gcagaacgtg ctgctcgccg ccgacggcac
     2761 ccccaagctg ggtgacttcg gcatcgcgcg cagcccggtg gatgccggcc tgaccgacac
     2821 cggcatggcg ctcggcaccg ccgcctacat ggcgcccgag caggcgaccg gcgccccgcc
     2881 cacgcccagc gccgatgtct acgccgccgg cgtgatcctc ttcgagctgc tgaccggacg
     2941 gctccccttc cccggcgaga acccggtcca ggtgatgtac cagcacgtca acaccccgcc
     3001 gcccaatccg cgcgccttca acccggctat cccgcccgcg ctgggcgcgg tcacgctgcg
     3061 cgccctggcc aaggacccgg ccacgcgctt cgccgacgcc cagcagatgc agtccgccat
     3121 cgacggtctg ccggccgccg acgccgccac cgcgatgctg cccaaggccg accctggccc
     3181 cgccgagcag acgcgggcca tgcgcgcggc cgccggaggg ggcgccggca atgccttgcc
     3241 tccgccgccg ccgccgccct accgccgcgt gctgcagagc agcggtcctg actggctacc
     3301 attcctgctg gccgggctcc tggtcgtgct ggtcatcggc ggcgtcatct tcctggcgag
     3361 ccacaacaac ggctcgccgg ccacaccgac cgtgccggtc gcggtcgcct cgccgaccgc
     3421 ga
//
LOCUS       DASUBI010000764         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1056059, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000764 DASUBI010000000
VERSION     DASUBI010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056059"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFU72_14940"
     CDS             <1..500
                     /locus_tag="VFU72_14940"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5424839.1"
                     /translation="AASALPDGWRSGRFGAGAGASWGKLGGRPAPGGLGTARASLPAG
                     ELAALLVVNALGEVVDQDTGQPLRPGSTDDAGRPPPAPGEHTTIGVLATTVPLDRAAL
                     VRLAIAAHDGLARAIRPAHTVFDGDTIIVLTTQEGHPTAEDVLLLGMVATDLVARAVA
                     NGATP"
     gene            591..1484
                     /locus_tag="VFU72_14945"
     CDS             591..1484
                     /locus_tag="VFU72_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5424840.1"
                     /translation="MKSDSTSSPTIICSTGSFAGQALSTRFRLARAAGTDGVELMLSP
                     RLVRGEPREVRRLEDEHHTPVRSVHSVLRLRDVSSEQAADDITTSARFAAQLHHCRVL
                     VVHPPQVDSLHDTAGRHWLEALEASAEIGARAGFAIAVENLGQLTRRGTASALDHPSR
                     LKWLAEEWDVALTFDTSHAGSRGWNLVEVSDLFGPRLANLHLSDLGARTYRWALTNAL
                     VRDHRLPGTGTLPLRSLLEHLASRHYSGLLTLELSPLALRAWWRPSTLARLRAAVLNC
                     RVQTHSSQPSSHPTTGPDPRG"
     gene            complement(1617..2006)
                     /gene="rpsI"
                     /locus_tag="VFU72_14950"
     CDS             complement(1617..2006)
                     /gene="rpsI"
                     /locus_tag="VFU72_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5424841.1"
                     /translation="MRYFQGVGRRKNAVARVRLYPGTGDIEVNAKPARDYFGGRDIYQ
                     VMLGEPLRLVNMIDRFNVRATVTGGGLSGQAGAVRHAVARALLHFEPELRPVLRRAGL
                     LTRDARVKERKKVGLKRARKAPQYTKR"
     gene            complement(2070..2516)
                     /gene="rplM"
                     /locus_tag="VFU72_14955"
     CDS             complement(2070..2516)
                     /gene="rplM"
                     /locus_tag="VFU72_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5424842.1"
                     /translation="MNTKTYSPTASEAQSQRQWFIVDAEGKTLGRLASQIAPILRGKH
                     KPTFATHMDMGDYVIVINADKFKVTGGKETSKFYYHHSQYPGGMRATALRDVLAKHPT
                     RPVEHAIKKMLPKNALGEAMFKKLKVYAGAEHPHGTQKPTPLHIEA"
     gene            complement(2578..>2823)
                     /locus_tag="VFU72_14960"
     CDS             complement(2578..>2823)
                     /locus_tag="VFU72_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5424843.1"
                     /translation="HWVALEQPIEPGEGARLLEFRIVAGGYLPQMVRNIVGDLVLIGR
                     GERPVGWLAELLRGHDRRLGAAPAPPEGLVLWHVSYT"
BASE COUNT          423 a         1012 c          910 g          478 t
ORIGIN      
        1 ccgccgcgag cgcgctgccc gacggctggc gctcaggtcg tttcggcgcg ggcgccggag
       61 catcctgggg caagctcggc ggccggccgg cgcctggtgg cctcggcacg gcgcgggctt
      121 ccctgccggc gggagagctc gcggcgttgc tggtggtgaa tgcgctgggc gaggtcgtgg
      181 atcaagatac gggccagccg ttgcgcccgg gatccacgga tgacgccggc cggccgcctc
      241 ccgcgcccgg cgaacacacc accatcggcg tcctggcgac cacggtgccg ctcgaccgcg
      301 ccgcgctggt gcggctggcc atcgccgccc acgacggcct ggcgcgcgcc attcgaccgg
      361 cgcacaccgt gttcgacggc gacacgatca ttgtcctgac gacgcaagaa ggtcatccga
      421 cagctgaaga cgtactgctg ctcggtatgg tcgccaccga cctcgtcgcg cgtgcggtgg
      481 cgaacggggc gacaccatga cggaacccga cgcatcgccc tgacgggcgg gcggacatgc
      541 tagactggaa ccgacggcat tcgcagccac acctgccatc gagtgctcgc atgaagagtg
      601 attcgacatc ctcgccaacg atcatctgct ccactggctc gttcgccggt caagcgttga
      661 gcacgcgttt tcgcctggcg cgtgcggctg ggaccgacgg cgtcgaactg atgctgagcc
      721 cgcggctggt gcgcggcgag ccacgcgaag tacgccgctt ggaggatgag caccacacac
      781 cggtccgctc ggtgcatagc gtgctacggc tgcgagatgt ctcctccgag caagctgccg
      841 acgatatcac tacctccgcg cgcttcgccg cgcaattgca ccactgtcgg gtgctggtcg
      901 tgcacccgcc ccaggtcgac agcctgcacg acacggcggg acggcactgg ctcgaagcgc
      961 tggaggcttc cgctgagatc ggggcgcgcg ccggcttcgc catcgcggtg gagaatctcg
     1021 gacaactgac caggcgaggc accgcctcgg cgctcgacca ccccagccgg ctgaagtggc
     1081 tggctgagga gtgggacgtc gcgctgacct tcgacacgtc ccacgccggc agccggggct
     1141 ggaatctggt cgaagtcagc gatctctttg gcccgcgcct ggcgaatctc cacctgagcg
     1201 acctcggcgc acgcacctat cgctgggcgc tgaccaacgc gctggtgcgc gatcaccggc
     1261 tgcccggcac aggtacgctg cccctgcgct ccttgctcga gcacctcgcg tcccgccact
     1321 atagcggact gctcaccctg gagctgagtc ccctggctct gcgcgcctgg tggcgaccgt
     1381 cgactctggc gcggctgcgc gcggcggtcc tgaactgccg tgtccaaacg cattcgtcgc
     1441 agccgtcgtc gcaccccact actggtcccg acccgcgcgg ttgatcgccg cgggaccctg
     1501 agtaccacag cggcaacgcg aaaagggcgg atttgacgtc cgccccgaca aatccaggta
     1561 tcccctctcc cacaactggc tcggtccgaa gaggggttgg gggtgagggc ccggacctag
     1621 cgcttggtgt actggggggc cttgcgcgct cgcttgaggc cgaccttctt acgctccttg
     1681 acgcgagcgt cgcgggtcaa cagaccggcc cgccgcagca ccggccgcag ctccggctcg
     1741 aagtgcagca gcgcccgcgc gaccgcatgg cgcaccgcgc ccgcctgccc gctcagcccg
     1801 ccgccggtca ccgtggcccg aacgttgaag cggtcgatca tgttcaccag tcgcagcggc
     1861 tcacccagca tcacctgata gatgtctcgc ccaccgaagt agtcgcgcgc cggtttggcg
     1921 ttcacttcga tgtcgccggt gccggggtag agacgcacac gcgcgacggc gttcttgcgc
     1981 cgccccacgc cctggaagta gcgcagcctg cgctcggtgt tggccggtcc cgtccctgtc
     2041 tccactgctt gctcttctcc tctgaattac tacgcttcga tatgcaacgg cgtcggcttc
     2101 tgagttccgt gggggtgctc cgcccccgcg tagaccttca gcttcttgaa catcgcctcg
     2161 cccagggcgt tcttcggcag catcttcttg atcgcgtgct cgaccgggcg cgtgggatgc
     2221 ttcgccagca cgtcgcgcag cgcggtggcg cgcatgccac cgggatactg cgagtgatgg
     2281 taatagaact tgctggtctc cttgccgccc gtcaccttaa acttgtcggc gttgatcacg
     2341 ataacgtagt cgcccatgtc catgtgtgtg gcgaaggtgg gcttgtgctt gccgcgcagg
     2401 atcggcgcga tctggctggc gagccgcccc agcgtcttcc cctccgcgtc gacgatgaac
     2461 cattggcgct gggattgggc ctccgacgcc gtcggtgaat aggtcttggt gttcatgcct
     2521 taagaactcc tcaatcgatc cgcgacgcca tgaccgatcc cggcgtcagt atccgcgcta
     2581 agtatacgag acgtgccaga gcacgagccc ctcgggcggc gccggtgccg cacccaacct
     2641 tcggtcgtgc ccgcgcagca gctccgccag ccatccgacc ggacgctcac cacgcccgat
     2701 cagcaccagg tcgccgacga tgttgcgcac catctgcggc agatacccgc ccgccacgat
     2761 gcggaactcc agcagcctgg cgccctcgcc tggctcgatc ggctgctcca gcgcgaccca
     2821 atg
//
LOCUS       DASUBI010000765         9039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1303179, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000765 DASUBI010000000
VERSION     DASUBI010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303179"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..750)
                     /locus_tag="VFU72_14965"
     CDS             complement(31..750)
                     /locus_tag="VFU72_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5424844.1"
                     /translation="MSRLGPLAAVLASVRAIRRPFRLLLVAVVLLGIGAACDHTNEYP
                     IDFFMEMHYEKFWRSQEPPVLDSPASAVPVSATVRQPLSYNWPAEPDKAVPNYSLDQA
                     KALTNPVPATDFNLKAGEALFYENCVQCHGAKGLGRDTPKADILPGVNQACDPTKPTV
                     CKGPGAGGTANAFLITYFESSGNIPPANLTSDAIKSLSDGELFYYVSNGLVDMPPFGN
                     LLTPDQRWALIHYIRHLQGQG"
     gene            complement(747..1313)
                     /locus_tag="VFU72_14970"
     CDS             complement(747..1313)
                     /locus_tag="VFU72_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU5424845.1"
                     /translation="MALRDVLGLYSDLDSAVAGADRLEEVGLGRPDFEVLSNAPFPEG
                     TFGHAPSIMRLGLWPLIGAAAGFSVGLLVTGGTQLVYPMVTDGKPLFSIPPMVIIMYE
                     GTMLAAVIFTVLGTLFESRLPRFQRAIYDTRITEGYIGLLVHAPSDSADRVAELLRST
                     GAEDVKMSEVVRGTIETPRQPRRKGANA"
     gene            complement(1326..2699)
                     /gene="nrfD"
                     /locus_tag="VFU72_14975"
     CDS             complement(1326..2699)
                     /gene="nrfD"
                     /locus_tag="VFU72_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5424846.1"
                     /translation="MENRQILPEDQVIAELLLPVERTTMWMWIAFIILGTILTAFVLV
                     VAWMFYWGVGVTGLNRPDMWGFMIVNFVFWIGISHAGVMISAILRLTQAEWRRPVTRA
                     AEVMTMFSLMTAMIFPFIHTGRPWRTAYWVFPYDFWRGIFPDVRSALVMDPSAVFTYL
                     TGTILFIYITTIPDIALLRDRSTGWRYQLYRIMAVGWQGGERQWVLQTISGFLLAALM
                     LSIFVSVHSIVSWDFSFSLDPVWHTPVWAPYFVIGAIHSGVSAVVTLMALMRWMFGFD
                     KYIREEHFDQIGRLLVAVATGWLWFFMLDVFFALYNQDPREVAIMEQRFLSWPYTMLV
                     GLIFIFGYFIPVPIWLFRKFRRNVKLMFWTSIMVNIGMWAERYWLVVPGLERKFEWTF
                     DWTFYRPGIDEITFIVGSFALVSCLFLLFAKLFPPVSVWEEKEGERFLREMQVGKRRI
                     PAIIREF"
     gene            complement(2731..4128)
                     /locus_tag="VFU72_14980"
     CDS             complement(2731..4128)
                     /locus_tag="VFU72_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424847.1"
                     /translation="MAAESHDNPQTTVMARVWDLTQPQVVRDGLSYLRGVSGAFRIWL
                     LISAVLGLVGVVALIIGPVLSGWGDRQPWTYVAVAFFYLMATVASAPIVVVSTRLTRG
                     HWRRPMARVSEMWAAAMIVPFILFLLLEPLLGGTGNRPSLWFGWWASPWLWDIVLMLG
                     LVIAGYGWLYISSIPDFAIARDMLEQGEGGRWTRLARGFYGTERNWAIFEKSVMVLGV
                     FYALLYIMTMTVLGADFIMSLVPGYKSAIFPAWLTISGFEGGIALVIVTATILRKWGG
                     ADEYLEREQFFALGKLQLAFGLLYFYFTWTDFIILWYGRMPWEIDLLHLLYFNRYGWL
                     FAPAVGLNFLWPLFLLVWTNVRRSINGPFYAACGVLIGTLFDRIRYLSSAFSTGNVDF
                     PVRELQSIPPTYYPDVWGVLVLIGMIFAAIAVVMLAFRVIPYPSIWEVTGGLWLRAKV
                     RIHHVEMMIIGKPDY"
     gene            complement(4150..4893)
                     /locus_tag="VFU72_14985"
     CDS             complement(4150..4893)
                     /locus_tag="VFU72_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5424848.1"
                     /translation="MANEARWGMVIDLDRCTGCQACVVACQAENNIPINTEENFHQRR
                     AIEWIRVERYWAGEFPDVKAYFIPVPCMQCEDAPCEPVCPVYATMHTSDGINMQVYNR
                     CIGTRYCSVNCHWHVRFFNFWEPKWPDSYKQYLNNMVTVRSRGVMEKCTFCVQRVNHA
                     RQVSELEGRPIDDSDFTTACAQACPSRAIVFGDANNPNSEVAQMIKSTDRGYRLLEDL
                     GTNPAVYYLKKVVMDERFVTHAEHAPAGA"
     gene            complement(4923..7175)
                     /locus_tag="VFU72_14990"
     CDS             complement(4923..7175)
                     /locus_tag="VFU72_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5424849.1"
                     /translation="MHRRDFLKLAAGSGAGSVLFIGCNATKYASTWGDGDPKHEFQIE
                     SPVLNPNDLQFHRDTWYATAYPGIGGGSGLVVRVFEGRAKKIEGNPIYPLNLGRSTAA
                     EQALLQELYHPDRLQTAQELETGAARGSGKFVPIKDWDAALAKFNGMLNNAKGKPNIL
                     LTGPVSGTTLAIINEFTGATGVKHLMLDRDENVVLREAMRRVFGTPSLPALDLAHANT
                     LVSFGADFLGEWISPVQFQIGYGDFRHARADTRGTFWYVGPQMTATAASADHWLPVRP
                     GTEGYVALAVANALGGSAAAALPGVSLDQFAPETVAAKVAAGGMNAKDVATRIRALAN
                     DFKHGPSISIGGGAAAAQTNGLFNVAAALALNLAGGNVGKPGGIILNPASPNTDLLPA
                     APAATPYRDLQGLANTLQGAGVVMVFDTNPVFTTPANPSLSQVLLASGAQIVSFSSFV
                     DETALVADLILPNHSTLESWGAFVPDPGPGYAVVALQQPVVEPFYATRQFTDILLDSV
                     KALGGTPKWATTQDAVTAAMTSLRGKGGNISAPTDIDYLVDAQSQGGWWDTGTRASGA
                     PAAPSNFPAPADPGFAGDPAQYPYYLVPFPHFTLGYGERAHLPWLQALPEPISTAVWT
                     TWVELNHHTADKIGVGIGDVVKLITPSGTIEAPVYVNPAAALDVALIPFGNGHTAGTR
                     YEEGRGVNPFRVLATQLEATTGALAWGATRVKIEKANKKVRLPLFQGSIQPYQEEGFP
                     IVQTMLPKTT"
     gene            complement(7190..7720)
                     /locus_tag="VFU72_14995"
     CDS             complement(7190..7720)
                     /locus_tag="VFU72_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5424850.1"
                     /translation="MAKFVKPLVLLIVVLLVLSVPAVAIVLRSYILAPTAQATTPQPI
                     TFPHNIHVQTVGLQCTFCHREATTTANATVPPLQLCMQCHSIIPTQNKPQLQKLVAAY
                     QNGDPVLWNKVTQLPDHVHFVHAMHIAAGFDCSTCHGAVWTMTTDKQVRDLRMGDCIV
                     CHRENNARTDCAVCHY"
     gene            complement(7793..8098)
                     /locus_tag="VFU72_15000"
     CDS             complement(7793..8098)
                     /locus_tag="VFU72_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424851.1"
                     /translation="MILLQVTHNPITAEQWSQITTAAVWLWSFVGCVIIMAGSILVGY
                     GIIPSLTSTRDLPARFYNFRVVFFALAGAFLLLAMFSLVEFITYMTVLYDIFNRVWI"
     gene            8342..8725
                     /locus_tag="VFU72_15005"
     CDS             8342..8725
                     /locus_tag="VFU72_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424852.1"
                     /translation="MAEPFDESYLRLCRRQGRSFVARLTARPGDWLLGAEGLRLAGDP
                     PEPAANELVVPDVERLLHLLRAEAPVIVLDVQQDDFGVTVFDEHGRSLANVVSRNAPE
                     ALLRALLFVRSERAGQGIPLTPVSG"
BASE COUNT         1561 a         2911 c         3154 g         1413 t
ORIGIN      
        1 gagaggagct gagggtgagg gccagtgtgt ctagccctgc ccttgcaagt gccggatgta
       61 gtggatcagg gcccagcgct gatccggcgt cagcaggttg ccgaacggcg gcatgtcgac
      121 caggccgttg ctcacgtagt agaacagctc gccgtcggag agggacttga tcgcgtcgct
      181 ggtcaggttg gccggcggaa tgttgccgct gctttcgaag taggtgatca ggaaggcgtt
      241 ggccgtgccg cccgcgccgg ggcccttgca gaccgtcggc ttggtcggat cgcacgcctg
      301 gttgaccccc ggcaggatgt ccgccttcgg cgtgtcgcgg cccagcccct tggcgccgtg
      361 acactgcacg cagttctcgt agaagagcgc ctcgccggcc ttcaggttga agtccgtggc
      421 cggcaccggg ttggtcagcg ccttggcctg atcgagcgag tagttgggca cggccttgtc
      481 cggctcggcc ggccagttgt acgacagcgg ctggcgcacg gtggccgaga ccggcaccgc
      541 gctggccggc gagtcgagca ccggcggctc ctgcgagcgc cagaacttct cgtagtgcat
      601 ctccatgaag aagtcgatcg ggtactcgtt ggtgtgatcg caggccgcgc cgatgccgag
      661 cagcaccacg gcgacgagca gcagcctgaa tgggcgccgg atcgcgcgca cggaggcgag
      721 caccgcggcc agcgggccga ggcggctcat gcgttcgctc ccttccgccg cggctggcgc
      781 ggagtctcaa tcgtgccgcg cacgacctcg gacatcttga catcctcggc gccggtcgag
      841 cggagcaact cggccacccg gtcggcgctg tcggacggcg cgtgcaccag cagcccgatg
      901 tagccctcgg tgatgcgcgt gtcgtagatc gcgcgctgga agcgcggcag gcgcgactcg
      961 aagagcgtgc cgagcacggt gaagatcacg gccgccagca tcgtgccctc gtacatgatg
     1021 atgaccatcg gcgggatcga gaagagcggc ttgccgtcgg tcaccatcgg gtagaccagt
     1081 tgggtgccgc cggtcaccag cagcccgacc gagaagccgg ccgccgcgcc gatcagcggc
     1141 cacagtccca ggcgcatgat gctgggcgcg tggccgaagg ttccctccgg gaagggcgcg
     1201 ttcgacagga cctcgaagtc cggccggccc aggccgacct cctccaaccg gtccgccccc
     1261 gcgaccgcgg agtcgaggtc ggaatagagc ccgagtacgt ctcgcagtgc catcgtcccc
     1321 tctccttaga actcgcggat gatcgccgga atgcgccgct tgccgacctg catctctcgc
     1381 aggaagcgct ccccttcttt ctcctcccag accgagaccg gcgggaagag cttggcgaag
     1441 agcaggaaca gacaactcac cagcgcgaac gagccgacga tgaaggtgat ctcgtcgatg
     1501 cccggccggt agaaggtcca gtcgaacgtc cactcgaact tgcgctccag acccgggacc
     1561 accagccagt agcgctcggc ccacatgccg atgttgacca tgatcgaggt ccagaacatc
     1621 agcttgacat tccgccggaa cttgcggaag agccagatcg gtaccgggat gaagtagccg
     1681 aagatgaaga tcaagccgac cagcatggtg tagggccagg agaggaagcg ctgctccatg
     1741 atcgccacct cgcgcgggtc ctggttgtag agcgcgaaga agacgtcgag catgaagaac
     1801 cagagccagc cggtggcgac cgcgaccagc agccgcccga tctgatcgaa gtgttcctca
     1861 cggatgtatt tgtcgaagcc gaacatccag cgcatcaggg ccatcagcgt gaccaccgcc
     1921 gagacgcccg agtggatggc cccgataacg aagtagggcg cccagaccgg ggtgtgccac
     1981 accggatcga gcgagaagga gaagtcccag gagacgatgc tgtggaccga gacgaagatc
     2041 gagagcatca gggccgccag cagaaagccg gagatcgtct gcagcaccca ctggcgctcg
     2101 ccgccctgcc agccgaccgc catgatgcgg tagagctggt agcgccagcc ggtcgagcgg
     2161 tcacgcaaga gcgcgatgtc cgggatggta gtgatgtaga tgaagaggat cgtgccggtc
     2221 aggtaggtga agacggcgct ggggtccatc accagcgcgg aacggacatc gggaaagatg
     2281 ccgcgccaga agtcgtaggg gaagacccag taggcggtgc gccaggggcg gccggtgtgg
     2341 atgaacggga agatcatggc cgtcatcagg ctgaacatcg tcatgacttc ggcggcgcgc
     2401 gtcaccggac ggcgccactc ggcctgggtc aggcgcagga tggccgagat catgacgccg
     2461 gcatgcgaga taccgatcca gaagacgaag ttgacgatca tgaagcccca catgtccggc
     2521 cggttcaggc cggtcacgcc gacgccccag tagaacatcc aggccaccac cagcacgaac
     2581 gcggtcagga tcgtgcccag gatgatgaac gcgatccaca tccacatcgt ggtgcgctcg
     2641 accggcaaga gcagttccgc gatgacctga tcttccggta ggatctgtcg attctccatg
     2701 cgcttcctcc ccgctccccg cggtggccct ttagtagtcc ggtttcccga tgatcatcat
     2761 ctcgacgtgg tggatgcgca ctttagcccg cagccagagt cccccggtga cctcccagat
     2821 cgaggggtag ggaatcaccc ggaaggcgag catcacgacg gcgatcgcgg cgaagatcat
     2881 accgatcagc accaggacgc cccacacatc ggggtaatag gtcggcggga tggattgcag
     2941 ctcgcgcacc gggaaatcga cgttcccggt cgagaaggcg gacgacaggt agcggatgcg
     3001 atcgaagagt gtgccgatca gcacgccgca ggccgcgtag aagggaccgt tgatgctgcg
     3061 gcgcacgttg gtccagacca gcaggaagag cggccagaga aagttcaggc cgaccgccgg
     3121 ggcgaagagc cagccgtagc ggttgaagta gaggagatgc agcagatcga tctcccacgg
     3181 catgcggccg taccagagga tgatgaagtc ggtccaggtg aagtagaagt agagcagccc
     3241 gaaggcgagc tgcagcttgc cgagggcgaa gaactgctcg cgctccaggt actcgtccgc
     3301 gccgccccac ttgcgcaaga tcgtcgcggt gacgatcacg agggcgatgc cgccctcgaa
     3361 gccgctgatc gtcagccagg cggggaagat agcgctcttg tagcccggca ccaggctcat
     3421 gatgaagtcg gcgcccagca cggtcatcgt catgatgtag agcagcgcgt agaagacgcc
     3481 cagcaccatg accgacttct cgaagatggc ccagttgcgc tcggtgccgt agaagccgcg
     3541 cgccagtcgc gtccagcggc cgccctcgcc ctgctccagc atgtcgcggg cgatggcgaa
     3601 gtccggaatc gagctgatat agagccagcc gtagccggcg atcaccagcc cgagcatcag
     3661 cacgatgtcc cacagccagg gcgaggccca ccagccgaac cagagcgagg ggcggttgcc
     3721 ggtgcccccc agcagcggct cgagcagcag gaagagaatg aagggcacga tcatggcggc
     3781 ggcccacatc tccgagacgc gggccatcgg acggcgccag tggccgcgcg tcagccgggt
     3841 ggagaccacc acgatcggcg ccgaggcgac ggtggccatc aggtagaaga aggcgaccgc
     3901 cacgtaggtc cagggctgcc ggtcgcccca gccagagagg accggcccga tgatcagggc
     3961 caccacgccg accagcccca gcacggcgct gatcagcagc cagatgcgaa aggccccgct
     4021 gacgccgcgc aggtacgaca gaccgtcacg cacgacctgc ggctgggtga gatcccagac
     4081 gcgcgccatc acggtcgtct gtggattgtc gtggctctct gctgccatag acctctctcc
     4141 cgcggctcgc taagcgccgg ctggcgcgtg ctcggcgtgc gtgacgaagc gctcgtccat
     4201 cacgaccttc ttgaggtagt agaccgccgg gttggtgccc agatcctcca gcagccggta
     4261 gccgcggtcg gtcgacttga tcatctgcgc gacctcgctg ttggggttgt tggcgtcgcc
     4321 gaagacgatc gcccggctcg ggcaggcctg cgcgcaggcg gtggtgaagt cgctgtcgtc
     4381 gatcggccgg ccctccagct cggagacctg gcgcgcgtgg ttgacgcgct gcacgcagaa
     4441 ggtgcacttc tccatcacgc cacggctgcg caccgtgacc atgttgttga gatattgctt
     4501 gtagctatcg ggccacttgg gctcccagaa gttgaagaag cgcacgtgcc agtggcagtt
     4561 gaccgagcag tagcgcgtgc cgatgcagcg gttgtagacc tgcatgttga tgccgtcgga
     4621 ggtgtgcatg gtggcgtaga ccgggcagac cggctcgcag ggcgcgtcct cgcactgcat
     4681 gcagggcacc gggatgaagt acgccttgac gtcggggaac tcacccgccc agtagcgctc
     4741 gacccggatc cactcgatcg cccgccgctg gtggaagttc tcctcggtgt tgatcgggat
     4801 gttgttctcc gcctggcagg cgacgacaca ggcctggcag ccggtacacc gatcgaggtc
     4861 gatgaccatc ccccagcgtg cttcgttcgc catcgtgact cctctcgccc ctgctacgct
     4921 cgctaggtcg tcttcggcag cattgtctgt acgatcggga agccctcttc ctggtagggc
     4981 tggatcgagc cctggaaaag cggcaggcgc accttcttgt tcgccttctc gatcttgacc
     5041 cgggtcgcgc cccaggccag cgcgccggtc gtcgcctcca gctgcgtcgc cagcacccgg
     5101 aaggggttga cgccgcggcc ctcctcgtag cgcgtgcccg cggtgtgccc gttgccgaag
     5161 gggatcagcg ccacatccag cgcggcggcc gggttgacgt agaccggcgc ctcgatcgtc
     5221 ccgctcggcg tgatcagctt gacgacgtcg ccgatgccga caccgatctt gtcggccgtg
     5281 tggtggttca gctcaaccca ggtggtccaa accgcggtcg agatcggctc cggcagcgcc
     5341 tgcagccagg gcaggtgcgc gcgctcgccg tagcccagcg tgaagtgcgg gaagggcacc
     5401 aggtagtagg gatactgcgc cggatcgccg gcgaagccgg ggtcggcggg cgccgggaag
     5461 ttgctcggcg ccgccggagc gccgctcgcc cgcgtcccgg tgtcccacca gccgccctgc
     5521 gactgggcgt cgacgaggta gtcgatgtcg gtcggggccg agatgttgcc ccccttgccg
     5581 cgcagcgagg tcatcgccgc cgtgacggca tcctgcgtgg tggcccactt gggggtgccg
     5641 ccgagcgcct tgaccgagtc gagcaggatg tcggtgaact ggcgcgtcgc gtagaagggc
     5701 tcgacgaccg gctgctgcag cgcgaccacc gcgtagcccg ggccgggatc gggcacgaag
     5761 gctccccagc tctccagcgt gctgtggttg ggcaggatca ggtcggcgac cagcgccgtc
     5821 tcatccacga aggacgagaa gctgacgatc tgcgcgccgc tggccaggag cacctggctc
     5881 agcgaagggt tggccggcgt cgtgaagacc gggttggtgt cgaagaccat caccaccccg
     5941 gcgccctgca gcgtgttcgc cagcccttgc aggtctcggt agggcgtggc cgccggagcg
     6001 gccggcagga ggtcggtatt gggcgaggcg gggttgagga tgatcccgcc cggcttgccg
     6061 acattgccgc cggcgagatt gagcgcgagg gccgcggcca cgttgaagag cccgttggtc
     6121 tgcgccgcgg ccgcgccgcc gccgatcgag atgctggggc cgtgcttgaa gtcgttggcc
     6181 agcgcccgga tgcgcgtggc gacgtctttg gcgttcatgc cgcccgccgc gactttggcc
     6241 gcgaccgtct cgggcgcgaa ttgatcgagc gacacgccgg gcaaggccgc cgccgcgctg
     6301 ccgccgagcg cgttggccac ggccagcgcc acgtagccct ccgtgccggg ccgcaccggc
     6361 agccagtggt cggccgaggc cgccgtggcc gtcatctgcg ggccgacgta ccagaaggtg
     6421 ccgcgcgtgt cggctcgtgc gtggcggaag tctccgtagc cgatctggaa ctgtaccggc
     6481 gagatccact cgcccaggaa gtcggcgccg aagctgacca gggtgttggc gtgggccagg
     6541 tcgagcgcgg gcagcgaggg ggtgccgaag acgcggcgca tcgcctcgcg cagcaccacg
     6601 ttctcgtcgc ggtcgagcat caggtgcttg acgccggtgg cgccggtgaa ctcgttgatg
     6661 atcgccagcg tcgtgcccga gaccggaccg gtcagcagga tgttcggctt ccccttggcg
     6721 ttgttgagca tgccgttgaa cttggccagc gcggcgtccc agtccttgat cggcacgaac
     6781 ttgccgctgc cgcgcgccgc gccggtctcc agttcctggg cggtctgcag acggtcgggg
     6841 tggtagagct cctgcaacag cgcctgctcg gcggccgtcg aacgccccag gttgagcggg
     6901 tagatcgggt tcccctcgat cttcttggcc cgcccctcga agacgcgcac caccaagccg
     6961 gagccaccgc cgatgccggg gtaggcggtg gcgtaccagg tgtcacggtg gaactggaga
     7021 tcgttcgggt tgaggaccgg gctctcgatc tggaactcgt gcttcggatc gccgtcaccc
     7081 caggtcgagg cgtacttggt ggcgttgcag ccgataaaga gtaccgaccc cgcccccgag
     7141 cctgccgcga gtttgagaaa gtcccgccga tgcatcgcac ccttccccat tagtagtggc
     7201 acaccgcgca gtcggtgcgt gcgttgttct cacggtgaca gacgatacag tcgcccatgc
     7261 gcaggtcgcg cacctgcttg tccgtggtca tcgtccagac cgcgccgtgg caggtcgaac
     7321 aatcgaagcc ggcggcgatg tgcatggcat gtacgaagtg cacgtggtcg gggagctggg
     7381 tcactttgtt ccagagcacg ggatcgccgt tctggtaggc cgcgaccagc ttctgcagct
     7441 gcggtttgtt ctgcgtcggg atgatcgagt ggcactgcat gcagagctgc agcggcggca
     7501 cggtggcgtt ggcggtggtg gtggcttcgc ggtggcagaa ggtgcactgg aggccgaccg
     7561 tctgcacgtg gatgttatgc gggaaggtga tcggctgcgg cgtcgtcgcc tgcgcggtgg
     7621 gagccaggat gtaacttcgg agaacgatgg cgaccgccgg caccgacaga accagcaaca
     7681 ccacgatcaa cagcaccagc ggtttgacga atttcgccat tcctccccgc tccactcctt
     7741 ggctcgatcc gtgccccggc gcggccactc gcgccgggcc agcgcgacga cgctagatcc
     7801 agacgcgatt gaagatgtcg tacaacaccg tcatgtaggt gatgaactcg accagcgaga
     7861 acatcgccag cagcaagaag gctccggcca gcgcgaaaaa gaccacgcgg aagttgtaga
     7921 agcgcgcggg gaggtcacgc gtggaggtca gcgaggggat gatgccgtag ccgaccagga
     7981 tgctgccggc catgatgatg acgcagccga cgaaggacca gagccagacg gccgcggtgg
     8041 tgatctgcga ccattgctcc gccgtgatcg gattgtgcgt gacctgcaat aggatcatcc
     8101 cgatcctccc ctgggctgcg cttggacctc gcggcgggtt gcgtgtgcct ggcgcaccga
     8161 ccggcgctca acaggcgcca aacgggctgc acggtcgtcc ccatgtgacc gtcttcacaa
     8221 ccctctggta gcatgaaggc aggcagaaaa gctgtcaaga ggggccggcc cgactgagcc
     8281 ggcagtgcct ggggggaatt tccccactgt cacacgagac gcgggagaga gagggcacgc
     8341 gatggccgag ccgttcgatg agtcgtacct acgcctgtgc cggcgccagg ggcgctcgtt
     8401 cgtcgcccgt ctgaccgccc gcccgggcga ctggctgctc ggggcggagg ggctgcgcct
     8461 ggcgggcgac ccgcccgagc cagcggcgaa cgagctggtg gtgccggatg tcgagcgcct
     8521 gcttcacctg ctgcgcgccg aggcgccggt gatcgtgctc gacgtgcagc aggacgactt
     8581 cggcgtgacg gtcttcgacg agcacgggcg ctcgctggcc aatgtcgtct cgcgcaacgc
     8641 gccggaggcc ctgctgcgcg ccctcctctt cgtccgctcc gagcgcgccg gccagggcat
     8701 cccgctgacg ccggtgtcgg ggtagaacgt gccctcaccc cccgcccctc tccggacgga
     8761 gccctgcggg ggcgagggga ggtggcgaac gacaaggagg ggcgtagagt ctctccacta
     8821 tggccaagcg tgctcgatgg gcagcgcccc tctccgtgcg gagccgcgca cgggagaggg
     8881 gtcgggggtg agggcactac gccaccgtct gcccgccgtc ggccaccagc agcgtgccgg
     8941 tgacgaagct ggcctcatcg ctggccagga agaggaccac gttggccagc tcctccggtg
     9001 cggcgtagcg gcgcagcgga atgcgcgaga ccgccgcgc
//
LOCUS       DASUBI010000766         7984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_210368, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000766 DASUBI010000000
VERSION     DASUBI010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210368"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFU72_15010"
     CDS             complement(<1..203)
                     /locus_tag="VFU72_15010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5424853.1"
                     /translation="MSARAHRLILVRHGETDYNRAGRYQGHLDIPLNELGLRQAEAIG
                     RRLAAWPLVRCYSSDLTRSLQTAR"
     gene            complement(208..750)
                     /locus_tag="VFU72_15015"
     CDS             complement(208..750)
                     /locus_tag="VFU72_15015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5424854.1"
                     /translation="MTSSNQPKQWSSPPAMQIDPNKTYQATLHTTKGDIVVDLLAKQD
                     PITVNNFVFLAEQGFYNNVPFHRIIKDFMIQTGDPTGTGRGGPGYRFQDEPVTGDYTP
                     GTVAMANAGPNTNGSQFFIMTQDYSGKLPKNYTIFGKVSQGMDVVQAIASTPVGPGAT
                     GEMSNPTEPVSIKSVDISSK"
     gene            complement(970..2571)
                     /gene="pckA"
                     /locus_tag="VFU72_15020"
     CDS             complement(970..2571)
                     /gene="pckA"
                     /locus_tag="VFU72_15020"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU5424855.1"
                     /translation="METITTLESSGGLEAHGINNPGAIYWSPPAAMLYEFAVRNGEGA
                     VAASGPLIVSTGKYTGRTPKDKFIVQEPGSNSAIWWDNNQPFAPERFDALHRRVSDYL
                     EGRDVYVQDCHTGADPDYRLKVRVITELAWHSLFVRNLFIRLHAGERFDGEPDFTVID
                     AAGFKAVPERDGTRGEAFIILNFAKRIILIGGTEYAGEMKKSIFSVMHYLLPRQGVFS
                     MHCSANAGESEDTALFFGLSGTGKTTLSTEPGRQLIGDDEHGWSDHGIFNFEGGSYAK
                     VIRLSSDSEPEIYQASHRFGAILENVVYNPVTRELDLNDDSLTENTRSAYPISFIPGA
                     SLTGTGSHPRNVFFLSADAFGVLPPISRLTPDQARYYFLSGYTAKVAGTEVGLTEPEA
                     TFSTCFGEPFLPLPPETYSRLLGEKIVKHNTNVWLVNTGWIGGTAAESERVPIAFTRA
                     MLRAAMDGLLEAIPTHEDPIFGLSIPDRCPNVPQHLLNPWEAWKNPEAYARMAQRLAQ
                     MFKANFEQVADNVPATVREAGPKTS"
     gene            complement(2750..3793)
                     /gene="queA"
                     /locus_tag="VFU72_15025"
     CDS             complement(2750..3793)
                     /gene="queA"
                     /locus_tag="VFU72_15025"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5424856.1"
                     /translation="MPDEPLLLRDYDYDLPEDLIAQTPVEPRDASRLLVLDRAGGRFE
                     HRVFREIGDYLAPGDLLVVNDTRVLPARLRGHRPSGGEVEILLLARRSPGLWQALVRP
                     GRRLRAGAEVVLTTLDGSRSEVAVIAAREPEGLALVRLPGPVEEDLAAFGEMPLPPYI
                     HQRLADPERYQTVYAEHRGSAAAPTAGLHFTPALLGELREQGVGMARLTLHVGIGTFR
                     PVKVEDAQQHEMHAEWYHVPAETLAAIQRTKASRGRIVAVGTTSCRTLESVDLTAPVD
                     EAQSGWTRLFITPGHRFQVVDQLITNFHLPRSTLLLLVSALASRETILAAYREAVRRR
                     YRFYSLGDAMLIR"
     gene            complement(3797..4837)
                     /gene="ruvB"
                     /locus_tag="VFU72_15030"
     CDS             complement(3797..4837)
                     /gene="ruvB"
                     /locus_tag="VFU72_15030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5424857.1"
                     /translation="MTTRVLSGKQRDEDGPVEGSLRPRRLAEYIGQDKVKDGLKIAIQ
                     AAQARGESLDHVLLYGPPGLGKTTLASIVAHEMGVNLRTTAGPAVERAGDLVSILTNL
                     KPGDVLFIDEIHRLSRVVEEVLYPAMEDYAVDIVLGKGPAARSMRLNLPRFTLVGATT
                     RLALLTSPLRDRFGSIYRLEFYDQPAMEEIITRSARILEVHLDAEAAREMALRARGTP
                     RIANRLLKRVRDFAQVRAEGAITVPVAREALDRLEIDALGLDDIDRRILKTLIELNDG
                     GPVGIETLAAATSEESDTIMDVYEPYLIQLGFMMRTPRGRVAARRAYEHLKLPPPARD
                     DFQASFFDPEQR"
     gene            complement(4995..6536)
                     /locus_tag="VFU72_15035"
     CDS             complement(4995..6536)
                     /locus_tag="VFU72_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424858.1"
                     /translation="MAEEGRQTGAASGTTEAAEPKRRLQEEAPVVTRHQVTIAGRALR
                     YTATAGFLPLRDDKDEIEAQIFFVAYTLDHEGEQATSERPLTFAFNGGPGSASIWLHL
                     GALGPRRVRLQPDGAMPAPPFRLIDNEDTWLARTDLVFVDPVGTGYSRAASAELSEKF
                     WGYQGDIDSLSEFIRLYLTRYERWTSPLFLAGESYGTLRAGGLSGRLIEMGIALNGIV
                     LISAVLSYLTVDMWTGQANDLPFALFLPSFAAAAWYHQKLPEALRHLPLTEFLDEVER
                     FAVDDYLTALVRGDGLPAEERQHMIAAVARRTGVDERYVDNSELRLQIHRFCKELLRD
                     RKLTVGRLDSRFTGIDVLAVTDVPDYDPSLVQPTPPFTATFNEYVRGQLRFRSDLEYE
                     TLNGEVNRKWRFDTETGRPGRRTGYLDTSRELRKAMVRNPYMKVLVAYGYYDLATPYF
                     GIKYTIDHLGLVHTRPERSLRGNVRYAAYEAGHMMYIDDGARRKLRDDVFAFVRDAMS
                     GDGQG"
     gene            complement(6635..6838)
                     /locus_tag="VFU72_15040"
     CDS             complement(6635..6838)
                     /locus_tag="VFU72_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU5424859.1"
                     /translation="MANEAKPISEELLAILACPVCKTPVRLEGEKLVCDNCGRRYRIE
                     DGIPIMLIDEAEMPENPARPPQG"
     gene            complement(6774..>7984)
                     /locus_tag="VFU72_15045"
     CDS             complement(6774..>7984)
                     /locus_tag="VFU72_15045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5424860.1"
                     /translation="YRQALERPGAIVLRHTFLHDLVLSVMVERREFSGYRTLMERRYG
                     APGREMAGRVLLGQHPLSVFAFPLIENVVEAAEQLVVQSRAAQDNVTARHPRARVRLI
                     PLGVRLPPAIDRRAARAQLALPADEFLLVSLAPADRRRRLDVVLDTVARLRRELPVRL
                     VLAGPVDPDLPLESLIGAAGLDGVAEAWGAVDRCTGSRLHAAADACLYLRSPWIGESS
                     VSLLRAMGAGRAAVVSDTGWFAELPNDAVAKVAVDVLEDETLCSLLRTFATEPELAQA
                     VGHRARAYVARAHSLQQMVAGFHQLLQELTGVDLPPPHGLPGVSLEVTAPNDDPARSP
                     DPLLDDVGAALAELGIGGGRWAREAARAAVELGLGSENDGANVQEERDGERGEADQRG
                     AARDPGLPGL"
BASE COUNT         1249 a         2729 c         2706 g         1300 t
ORIGIN      
        1 cgcgccgtct gcagcgagcg cgtcaggtcg ctggaatagc agcgaaccag cggccacgcg
       61 gccaggcggc ggccgatcgc ctccgcctga cgcaggccta gctcattcaa cgggatatcg
      121 agatgaccct ggtagcgtcc ggcgcggttg taatcggtct cgccgtggcg caccaggatc
      181 agccggtggg cacgagcact caccgggtta cttgctgctg atgtcgacac tcttgatcga
      241 caccggctcg gtcggattcg acatctcgcc ggtggcgccg gggccaaccg gcgtgctggc
      301 gatcgcctgc accacgtcca tcccttgcga caccttgccg aagatggtgt agttcttcgg
      361 cagcttgccg ctgtagtcct gggtcatgat gaagaactgg ctgccgttgg tgttggggcc
      421 ggcgttggcc atcgccacgg tgccgggcgt gtagtcgccc gttaccggct cgtcctggaa
      481 acggtagcca gggccgccac ggccggtgcc ggtcgggtcg ccggtctgaa tcatgaagtc
      541 cttgatgatg cggtggaagg gcacgttgtt gtagaagcct tgctcggcca ggaagacgaa
      601 attattgacc gtgatcgggt cctgcttggc caggagatcg accacgatgt cacccttggt
      661 ggtgtgcagc gtagcctggt aggtcttgtt ggggtcgatc tgcatcgccg gcggcgacga
      721 ccactgcttc ggctggttgc tcgatgtcac tgcggctcca ttcccactct cggtcgcgtt
      781 ggtcgtcgcg gcgcttgtcg ccaatggcgt cgtcttcgac gcctgcgctg agtccccgca
      841 ccccgacaac accaagacgg ccgtggccag tatgccgaat agcgcccacc gccacggccg
      901 tatcagaatc aatcgcaccg gcgactactc cctagcgggg ctgccgcccg cgcgggagac
      961 agccctgaat cagctcgtct tcggaccggc ctcgcgcacg gtcgccggca cgttgtccgc
     1021 gacctgctcg aagttcgcct tgaacatttg agccagtcgc tgcgccatgc gcgcgtacgc
     1081 ctccggattc ttccacgcct cccacgggtt cagcaagtgc tggggcacgt tggggcagcg
     1141 gtccgggatg ctcagcccga agatcggatc ctcgtgcgtc gggatcgcct ccagcaaccc
     1201 gtccatcgcc gctcgcagca tggcgcgggt gaaggcgatc ggcacgcgct ccgactctgc
     1261 agccgtgccg ccgatccagc cggtgttgac cagccagacg ttggtgttgt gcttgacgat
     1321 cttctcgccg agcagcctgg aataggtctc cggcggcagc ggcaggaagg gctcgccgaa
     1381 acaggtactg aaggtggctt ccggctcggt cagccccacc tcggtgccgg cgaccttggc
     1441 ggtgtagccg gagagaaagt agtagcgcgc ctggtcgggc gtcagccgcg agatcggcgg
     1501 cagcacgccg aaggcatcgg cgctgaggaa gaagacgttg cgcgggtgcg agcccgtgcc
     1561 ggtcaacgag gcgccgggga tgaaggagat cgggtaggcc gagcgcgtgt tctcggtcag
     1621 cgagtcgtcg ttgagatcga gctcgcgcgt gaccgggttg tagaccacgt tctccaggat
     1681 cgcgccgaag cggtggctgg cctggtagat ctccggctcg gaatcggagg agagccggat
     1741 caccttggca tagctaccgc cctcgaaatt gaagatgccg tggtcgctcc agccgtgctc
     1801 atcgtcgccg atgagctggc gccccggctc ggtggagagc gtggtcttgc cggtgcccga
     1861 gaggccgaag aagagcgccg tgtcctcgga ttcgccggcg ttggccgagc agtgcatcga
     1921 gaagacgccc tgccgcggca gaagatagtg catcacgctg aagatcgact tcttcatctc
     1981 gccggcgtac tcggtgccgc cgatcaggat gatacgcttg gcaaagttga gaatgatgaa
     2041 ggcttcgccg cgcgtcccat ctcgctcggg gacggccttg aagccggccg cgtcgatgac
     2101 ggtgaagtcc ggctcaccat cgaagcgctc cccggcgtgg agccgaatga agaggttgcg
     2161 cacgaagagg ctgtgccagg ccaactcggt gatgacgcgc accttcagcc ggtagtctgg
     2221 gtcggcgccg gtgtggcagt cctgcacgta gacgtcgcgg ccttcgaggt agtcgctgac
     2281 ccgccggtgc agcgcgtcga acctctcagg cgcgaagggc tggttattgt cccaccaaat
     2341 ggcgctgttc gagcccggct cctggacgat gaatttgtcc ttgggggtgc gtccggtgta
     2401 cttgccggtc gagacgatga gcgggccgct cgcggcgacc gccccttcgc cgttgcgcac
     2461 cgcgaattcg tagagcatgg ccgccggcgg cgaccagtag atcgcgccgg ggttattgat
     2521 cccgtgcgct tcgagtccac cgctggactc caacgtcgtg atggtctcca tcgatcctcc
     2581 caccgctagc tccggcgacc cactccgtca gcaccccatc gtggacggct gcctggtcgg
     2641 gagcgctccc gcgcgacggt ccatccacga tgctacaccg catccggacg accggcaacg
     2701 actccacgct ggccggaggc caatggagca atgcgcgcgc cagcgctcct cagcggatga
     2761 gcatggcgtc gcccaggcta tagaagcggt agcgccgccg caccgcttcc cgataggccg
     2821 ccagaatcgt ctcgcggctg gccagggcgc tgaccagcaa gaggagtgtg gagcgcggca
     2881 ggtggaaatt ggtgatgagt tggtcgacga cctgaaagcg gtgccccggc gtgatgaaga
     2941 gccgggtcca acctgactgc gcctcgtcga ccggtgcggt caggtcgacc gactccagcg
     3001 tacggcagct cgtggttccc accgcgacga tgcggcccct ggacgccttc gtacgttgga
     3061 tggcagccaa cgtctccgct ggcacgtggt accactcggc gtgcatttcg tgttgctggg
     3121 catcctcgac cttgacgggg cggaacgttc cgatgccgac gtgcaaggtc agccgcgcca
     3181 tgccgacgcc ttgctcgcgc agttcaccca gcagcgcggg cgtgaagtgc agcccggcgg
     3241 tcggcgccgc cgccgatcca cggtgctcgg cgtagacggt ctgatagcgc tcggggtccg
     3301 ccagccgctg gtgaatgtag ggcggcagcg gcatctcgcc gaaggctgcc aggtcttcct
     3361 cgaccggccc cggtaggcgg accagcgcca gcccctccgg ctcgcgcgcc gcgatcacgg
     3421 cgacctcgct ccggctgccg tctaacgtcg tcagcacgac ttcggcgccc gcacgcagcc
     3481 ggcgccccgg gcgcaccagt gcctgccaga gccccggcga ccgacgcgcc agcagcagga
     3541 tctccacctc tccgccgctc gggcgatgcc cgcgcagccg cgccggcagc acgcgggtgt
     3601 cgttgacgac cagcagatcg cccggcgcga ggtagtcgcc gatctcgcga aagacgcggt
     3661 gctcgaagcg gccgccggca cgatcgagca ccagcagccg cgaggcatcg cgcggctcga
     3721 ccggcgtctg ggcgatcaga tcctcgggca ggtcatagtc atagtcgcgc aggaggagcg
     3781 gttcgtccgg catcagctag cgttgctcgg ggtcgaagaa ggaggcctgg aaatcgtcgc
     3841 gcgccggcgg cggcagcttc aggtgctcgt aggcgcgacg ggcggcgacc cgaccgcgcg
     3901 gggtgcgcat catgaagccg agctggatga gatagggctc gtagacatcc atgatggtgt
     3961 cggactcctc gctggtggcc gccgccagcg tctcgatccc gaccggacca ccgtcgttca
     4021 gctcgatcag cgtcttcagg atgcgccggt cgatatcgtc cagccccagc gcgtcgatct
     4081 ccagccggtc gagcgcctcg cgcgccaccg gcacggtgat cgcgccctcg gcccgcacct
     4141 gggcgaagtc acgcaccctt ttgagcagcc ggttggcgat gcgcggcgtg ccgcgcgccc
     4201 gcagcgccat ctcgcgcgcg gcctcggcat cgagatgcac ctccaggatg cgggccgagc
     4261 gcgtgatgat ctcctccatg gccggctggt cgtagaactc cagccgatag atcgaaccga
     4321 aacgatcgcg cagcggtgag gtcaagagcg ccagccgcgt ggtagctccc accagggtga
     4381 agcgcggcag attcaagcgc atgctgcgag cggccggccc cttgcccagc acgatatcga
     4441 cggcgtagtc ctccatcgcc gggtagagaa cctcctccac cacgcggctg aggcggtgga
     4501 tctcgtcgat aaagaggaca tcgcccggct tgaggttggt caggatcgag accaggtcgc
     4561 cggcacgttc gacggccggg ccggcggtgg tgcgcaggtt gacgcccatc tcgtgcgcca
     4621 cgattgaggc caacgtggtc ttgcccagcc cgggagggcc gtagagcagc acgtggtcga
     4681 gcgactcgcc gcgcgcctgg gcggcctgga tggcgatctt cagcccatcc ttgaccttgt
     4741 cctgaccgat gtactcggcc agccggcgcg gacgcaggct cccttcgacc ggtccgtcct
     4801 cgtcgcgctg cttgccgctg agcactcgtg tggtcatcgc cgtcgccgct ccctcgttcg
     4861 gcgtcaagta tagcagacgg gttagaacat atgtacgtat ttgacgagcg caatcgatcg
     4921 tgcggcaccg cgcggcggcc tcagcccgcg cgtcacgtgc gacccaacgt catcgacggg
     4981 caccgtaccc cgcctcagcc ctgaccatca ccggacatcg cgtcccgcac gaaggcgaag
     5041 acgtcgtcgc gcagcttgcg acgggcgccg tcgtcgatgt acatcatgtg gcccgcctcg
     5101 taggccgcgt agcgcacgtt gccgcgcagc gagcgctcgg gccgcgtgtg caccagcccc
     5161 aggtggtcga tcgtgtactt gatgccgaag tagggcgtgg ccaggtcgta gtagccgtag
     5221 gccaccagca ccttcatgta ggggttgcgc accatggcct tgcgcagctc gcggctggta
     5281 tcgagatagc cggtgcgcct gccggggcga cccgtctcgg tatcgaagcg ccacttgcgg
     5341 ttgacttcgc cgttcagcgt ctcgtactcc aggtcgctgc ggaagcgcaa ttgcccgcgt
     5401 acgtactcgt tgaatgtagc ggtaaagggc ggcgtgggct gcaccaggct ggggtcgtag
     5461 tccggcacgt cggtcaccgc cagcacgtcg atgccggtga agcggctgtc gagccggccg
     5521 acggtgagct tgcggtcgcg cagcagctcc ttgcagaagc ggtggatctg caagcgcagt
     5581 tcgctattgt cgacgtagcg ctcgtcgacc cccgtgcgcc gcgccaccgc cgcgatcatg
     5641 tgctggcgct cctcggccgg cagaccgtcg ccgcgcacca gcgcggtcag atagtcgtcg
     5701 acggcgaagc gctcgacctc gtccaggaac tcggtcagtg gcaggtgccg cagcgcctcc
     5761 ggcagcttct ggtggtacca cgccgccgcg gcgaacgagg gcaggaagag ggcgaagggg
     5821 aggtcgttcg cttgcccggt ccacatgtcg accgtcagat aggataggac ggccgagatc
     5881 aacacgatgc cgttgagggc gatgcccatc tcgatgagcc ggccggagag accgccggcg
     5941 cgcagcgtgc cgtagctctc gccggccaga aacagcggcg aggtccagcg ctcgtagcgg
     6001 gtcaggtaga ggcggatgaa ctcgctgagc gagtcgatgt cgccctggta gccccagaat
     6061 ttctcggaga gctccgctga cgcggcgcga ctgtagccgg tgccgaccgg atcgacgaag
     6121 accagatcgg tgcgcgccag ccaggtgtcc tcgttgtcga tcaggcggaa cggcggcgct
     6181 ggcatggcgc cgtcgggctg caggcgcacg cgccgtggac cgagcgcgcc gagatggagc
     6241 cagatcgaag ccgagccggg accgccattg aaggcgaagg tcaggggacg ctcggacgtc
     6301 gcttgctcgc cctcgtggtc gagtgtgtag gccacgaaga agatctgcgc ctcgatctcg
     6361 tccttgtcgt cgcgcagcgg caggaagccg gcggtagcgg tgtagcgcag cgctcgcccg
     6421 gcgatcgtca cctgatgccg ggtgacgacc ggcgcctcct cctgcaagcg ccgcttcggc
     6481 tcggcggcct cggtcgtgcc gctcgccgcg ccggtctggc gcccctcctc ggccactcat
     6541 ttcccccgtt cgctctgttc gtacgtaacg tccgtacact cgaccatcga gacgctgtcg
     6601 gctgtagaag tgcgtacgaa cgcgaatcct tggcctatcc ctgagggggc cgcgccgggt
     6661 tctccggcat ctcggcctcg tcgatcagca tgatcgggat gccatcctcg atgcggtagc
     6721 gccggccgca gttgtcacag accagcttct ctccctccag ccgcaccggc gtcttacaaa
     6781 ccgggcaggc caggatcgcg agcagctcct cgctgatcgg cttcgcctcg ttcgccatcg
     6841 cgttcctcct gtacgttcgc accatcgttc tcgctgccca gccccaactc gaccgccgcc
     6901 cgcgccgcct cgcgcgccca gcgcccgccg ccgatcccca actccgccag cgccgcgccg
     6961 acgtcatcaa gcagtggatc gggcgaccgc gccgggtcgt cgttcggcgc cgtcacttcc
     7021 aacgacacgc ccggcaggcc gtgcggcggc ggcagatcca ccccggtcag ctcctgcaac
     7081 agctgatgga agcccgccac catctgctgc aggctgtgcg cgcgcgcgac ataggcgcgc
     7141 gcgcggtgcc caaccgcctg cgccagctcc ggctcggtgg cgaatgtccg cagcagcgag
     7201 cataacgtct catcctccag cacgtcgacc gccaccttgg ccaccgcgtc gttcggcagc
     7261 tcggcaaacc acccagtgtc gctgacgacg gctgcgcgcc cggcgcccat cgcgcggagc
     7321 aggctgaccg agctctcgcc gatccagggt gagcgcaggt agaggcaggc gtcggcggcc
     7381 gcatgcagcc ggctccccgt gcatcgatcc actgcgcccc aggcctccgc caccccgtcc
     7441 aggccggccg ctccaatcag cgactccagc ggcaagtcgg gatcgaccgg ccccgcgagc
     7501 accagccgca ccggcagctc gcgccgcagc cgggcgaccg tgtcaagcac gacgtccagc
     7561 cgccggcgcc ggtcggccgg agcgagcgag acgagcagga actcgtccgc cggcaacgcc
     7621 agctgcgcac gcgccgcccg ccggtcgatg gcgggcggca gccgcacgcc gagcggtatc
     7681 aggcgcacgc gcgcccgcgg atggcgcgcc gtcacgttgt cttgcgcggc ccggctctgc
     7741 acgacgagtt gctcggcggc ctcgacgacg ttctcgatca atggaaaggc gaagacggag
     7801 agtggatgct gaccgagcag tacccgcccg gccatctcac gccccggcgc tccgtagcgc
     7861 cgctccatca gcgtgcggta gccgctgaac tcgcgccgct cgaccatgac cgacaggacg
     7921 agatcgtgca ggaaggtgtg ccggagcacg atcgccccgg gccgctcgag ggcctgccgg
     7981 tagc
//
LOCUS       DASUBI010000767         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_196650, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000767 DASUBI010000000
VERSION     DASUBI010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196650"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFU72_15050"
     CDS             <1..437
                     /locus_tag="VFU72_15050"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5424861.1"
                     /translation="GKTSRIWKPTRDSAGHDIINIRFDYADCRACPARQRCVSSDRER
                     ALAIRPQPLYEALRDARQRQHTPAFRAQYHARAGIEGTFSQGVSLGDLRRTRYRGLAK
                     TRLLHLFIACALNLLRIAAWLAERPRAHTRTSAFAALAPVCG"
     gene            597..1577
                     /locus_tag="VFU72_15055"
     CDS             597..1577
                     /locus_tag="VFU72_15055"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU5424862.1"
                     /translation="MPTATLVPDPAVVTLEEIGIDDRGITLLARTRQAVACCPVCDQP
                     ATRIHSWYTRRLDDLPWQGLAVRLHFRTRRWFCDNPHCDRRIFTERLPTIAAPHSRRT
                     QRLATIVLVFGVAVGGAPGAHLLAALGIGVSDDTLRRAVGRAALPEAATPRVLGVDDW
                     SLRKGCMYGTILVDLEERRPVDLLPDRSAAGLEAWLMAHPGVEIICRDRSGAYADGAR
                     QGAPDAIQVADRWHLFANLATCWNACWCGTTPRWGRCAWRSPSRQRRRPSQPPHPAHR
                     LCRHRPHPGRRPAPNKSASAASTGGRRVTARSTRSRRRGTASGPLPNSCT"
     gene            1568..2266
                     /locus_tag="VFU72_15060"
     CDS             1568..2266
                     /locus_tag="VFU72_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5424863.1"
                     /translation="MHLSRTTVGKYLAAPTCPHPGPRPQRQRRITPFVPYLRQRWTAG
                     ERRPRVLWDEIRAQGFPGSWRRVQEQTAVWRRAERQAHLGSAEGTPITARPAQPAGKR
                     CAPRQVATWLVRAPADLRPAQRAYLEALIAACPAIGVAQDLAQDFVRLLRTREVAGLA
                     PWLVRAEECTVTEVREFAAGIRRDQPAVQAALAYAWNSGQVEGQVNRLKLVKRSMYGR
                     ASFALLRQRFLLAA"
BASE COUNT          364 a          961 c          829 g          365 t
ORIGIN      
        1 aaggaaagac cagcagaatc tggaaaccga cgcgcgatag cgccggccac gatatcatca
       61 atatccgctt tgactatgcc gactgtcgcg cctgcccagc ccgccagcgg tgcgtgtcct
      121 ccgaccgcga gcgcgcactc gctatccgtc cgcagccact ctatgaggcg ctccgcgacg
      181 cgcggcagcg ccagcacacg ccggcgttcc gcgcgcagta ccacgcccgg gccgggatcg
      241 aaggcacctt ctcgcaaggc gtaagtctgg gcgacttacg ccggacgcgc tatcgcgggc
      301 tggcgaagac gcgactcctg cacctattca tcgcctgcgc cctcaatctc ctacgcatcg
      361 ccgcctggct cgccgagcgc ccccgtgccc acacccgcac ctctgccttc gccgccctgg
      421 caccggtctg cggctaactg gccgctctgc tcagtcgggg ttcgccagca gcatctgtgc
      481 aagtggttca tcagcaaagt tggtgagccc ggcccgcccc cgcctggccc gctcagcgct
      541 gcgcatgtgc gacaatcggt ccttgctccg ccgccacttg caggaggccc gccgccatgc
      601 ccaccgccac cctcgtcccc gacccggccg ttgtcacgct ggaggagatc ggcatcgatg
      661 atcgcggcat cacgctcctc gctcgcacac ggcaagcggt ggcctgctgc ccggtgtgcg
      721 accagcctgc gacgcgcatc catagttggt atacccggcg gcttgatgac ctgccgtggc
      781 aggggttggc cgtccggctc cacttccgga cccgccgctg gttctgtgac aacccgcact
      841 gcgaccggcg catcttcacc gagcgcctgc cgacaatcgc cgcgccgcac agccgacgga
      901 cccagcgcct cgccaccatt gtccttgtct tcggcgtcgc cgtcggtggc gcacccggtg
      961 cgcatctact ggctgcactc ggcatcggcg tgagcgacga cacgctgcgc cgggcggtcg
     1021 gccgtgccgc cctgcctgaa gccgcgacac cacgcgtgtt gggggtggac gattggagcc
     1081 tgcgcaaggg gtgtatgtat ggaaccatcc tcgtcgattt ggaggaacga cgccccgtcg
     1141 acctcctccc cgatcggtct gccgccggtt tggaagcctg gctcatggcg caccccggtg
     1201 tcgaaatcat ctgccgtgat cgcagtggcg cctacgccga cggggcgcgg caaggcgccc
     1261 cggacgccat ccaggtcgcc gaccgctggc acctcttcgc caatctggcg acctgctgga
     1321 acgcctgctg gtgcggcacc acgccgcgct ggggcaggtg cgcttggcgg agcccgtcac
     1381 gccagcgccg gaggccgtcc cagccgcccc acccggccca ccgcctgtgc cgacaccgcc
     1441 cgcacccagg ccgccgaccc gcacccaaca agagcgccag cgccgcatcg accggcggga
     1501 ggcgcgtcac cgcgagatcc acgcgctcca ggcgcagggg cacagcatcc gggccattgc
     1561 ccaacagttg cacctgagcc gcaccacggt cggcaaatac ctcgccgccc caacctgtcc
     1621 ccaccccggc ccgcgcccgc agcgccagcg gcgcatcacg cccttcgtgc cctacctgcg
     1681 gcagcgctgg acggccggcg aacggcggcc gcgcgtcctg tgggacgaga tccgcgccca
     1741 gggtttcccg ggctcctggc ggcgcgtgca ggagcagacc gcggtctggc gccgcgcaga
     1801 gcggcaggca cacctgggca gcgccgaggg gacacccatc accgcgcgtc ccgcgcagcc
     1861 ggcgggcaag cgttgtgcgc cgcgtcaggt tgccacctgg ctcgtccgtg ccccggccga
     1921 tctccggccg gcgcagcgag cctatctcga ggccctgatc gccgcctgcc cggccattgg
     1981 tgtcgcgcag gatctggcac aggacttcgt gcgcctcctc cgcacgcggg aggtcgccgg
     2041 gttggcaccg tggctggtcc gggccgagga gtgcaccgtg actgaggtgc gtgaattcgc
     2101 cgccggcatc cggcgcgacc aaccggcggt ccaggccgcc ctggcgtacg cctggaactc
     2161 ggggcaggtc gagggccagg tgaaccgcct gaagctggtc aaacggagca tgtacgggcg
     2221 cgccagcttc gcgctcctac gccagcgctt cctcctggct gcctgatgtg ggctgattca
     2281 ggccgctggc atgatgccgc ccagtggtgc ttgtccgggc gagtcatcct gcccgcaagc
     2341 gggagccgag cgggccaggc gggggcgggc cgggctcacc aactttgctg atgaacccgg
     2401 gaaggtgacg gtgtgccggc cgtcacgcac ctgccacacg acggtgtcgc ccgcctggat
     2461 gacgaggaga gcgggggcaa acgcgggggc acacgtcggc gctggcgcct cccaggcac
//
LOCUS       DASUBI010000768         5888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1090208, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000768 DASUBI010000000
VERSION     DASUBI010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090208"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFU72_15065"
     CDS             complement(<1..413)
                     /locus_tag="VFU72_15065"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEU5424864.1"
                     /translation="MKNLSRRFARIFPGTGKPLIAIVNLPPLPGTPAYNAAAGMDGIL
                     AAARRDLDTLLAQPEIDGILLCNTGDRPYSLRADLAGVAALARVIGALAPRDRPFGIE
                     FRCDARAALAVAVATGAAFIRAVAGGLYASDMGLW"
     gene            555..1928
                     /locus_tag="VFU72_15070"
     CDS             555..1928
                     /locus_tag="VFU72_15070"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5424865.1"
                     /translation="MSRLTCLSVIGLLGLGSLLAACSSGGSPASPAPSAAALASPPAA
                     ATAPATTAATAAPASPTAVATATAAEASATQPPSPTATAVPTTAPSSTPTPAPTVPSG
                     PPQAHQVAGGTIMLPAGFNIGVFAEDLGGPRFMAVRPSDGLLFATDMRGGRILVLPDA
                     NHDGKADSVQVFASGLNLPSSLAFYQDWLYVGETNQVSRFRAPQGALKPEGGKEVVVP
                     NLPSGGGHFTRTVAFSPDGRLFISVGSSCNVCVESDNRRAAILVANADGSQLRLYATG
                     LRNAVGLAFQPGTNQLWATVNGRDNLGDNLPPDDLRRIEDGVFYGWPYCYDGEHPNPE
                     FNDSGKCAGVPPDTVALPAHSAALGLTFGQGLQAPQPYQDSIYIAYHGSWNRSIPTGY
                     KVVRVPLVNGQPGQPEDFAWGWLPGSAQNPGQVWGRPVGVTVGSDGALYVSDDSTGLV
                     YRIWFGG"
     gene            complement(2141..2986)
                     /locus_tag="VFU72_15075"
     CDS             complement(2141..2986)
                     /locus_tag="VFU72_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5424866.1"
                     /translation="MSDVMRVGLVQINTQADKEANLAKAERLIDEAAGNGARLVALPE
                     YVAYLGPKEAHEAVAEPIPGPTTARFAAKARQHGIYLLGGSIHERSDVPGKYYNTSVF
                     YGPDGAMLATYRKIHLFDIDLTGNVSANESATILPGEDVVTAEAGGHTLGLTICYDLR
                     FPELYRLLALAGAEVLCVPAAFTLYTGKDHWHALLRARAIENQCYVIAPAQIGPHEPH
                     ARCYGHSLVVDPWGTVIADAPDREGVVTAELDFAYLRQLRAQLPSLANRRPAAYRARE
                     AVLTS"
     gene            3130..3384
                     /locus_tag="VFU72_15080"
     CDS             3130..3384
                     /locus_tag="VFU72_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424867.1"
                     /translation="MHEVFPVAAGLLVGALAYRVSSPRLRALVMAVLSVLLGVAATFI
                     SGEALISWGFVLIDIPLVLGASVIGYGLLVLWQRWSTQPR"
     gene            complement(3444..4169)
                     /locus_tag="VFU72_15085"
     CDS             complement(3444..4169)
                     /locus_tag="VFU72_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5424868.1"
                     /translation="MSALWAQTRMELMLTVRRFEGVLITMVVPVVLLVFFAAIGLKPD
                     KFERPIDFLLPGMLALAVMSTGLVSLAIRTAYERNYGVLKRLGSTPLTRAQLLGAKVI
                     SVIVIELFEIVLLIGLSWAFYDWHPSGALWLAVLAMILGTATFSLFGLMLAGAVRAET
                     TLAAANALYLLFLLLGGIVWPVDRLPGLLSLPARLLPSSAFADALRASLGSHPTFPLS
                     NLVILAGWALLALVVAARTFQWE"
     gene            complement(4305..5201)
                     /locus_tag="VFU72_15090"
     CDS             complement(4305..5201)
                     /locus_tag="VFU72_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424869.1"
                     /translation="MRDLVKSYGDKRAVNGLSFSVCRGEIFALLGPNGAGKTTTVEIL
                     EGYRGADGGEVRVLGLDPQRQGGELKRRIGVMLQNAGIYPTVTPLEILELFRHFYPES
                     EEPRALLQLVGLEESADVRFRQLSGGQQRRLALAAALIGKPELLFLDEPTTAMDPQAR
                     HVTWDILQSLRARGLTVLLTTHFMEEAERLADRVAIIDHGKLLALEEPRVLTATSNEE
                     VQFSGPAGLDVGGLAALPATEGAREVRPGFYVVQTRRPIDLLAEVTAWARERDVLLND
                     LRVGHESLEEVFLRLTGHELRE"
     gene            complement(5246..5671)
                     /locus_tag="VFU72_15095"
     CDS             complement(5246..5671)
                     /locus_tag="VFU72_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU5424870.1"
                     /translation="MAHWLPLIDTSLILISGVALLIGYRFIRRGEIQRHKWSMLTATS
                     FAALFLIVYLLRSALYGSKLFAGHGAVRVVYLTILTTHTILATAIVPIVLVVLYRALT
                     RQFPRHKRLARVALPIWLYVVVTGWVIYMMLYQLSFTRT"
BASE COUNT          881 a         2013 c         2105 g          889 t
ORIGIN      
        1 ctccagagcc ccatatccga ggcgtagagg ccgcccgcga cggcgcggat gaaggcggcg
       61 ccggtggcga ccgcgaccgc tagcgcggct cgggcgtcgc agcggaattc gatgccgaac
      121 gggcgatcgc gaggcgccag cgcgccgatg acgcgcgcca gcgcggccac gccggcgaga
      181 tcggcccgga ggctgtaggg gcggtcgccc gtattgcaca agaggatgcc gtcgatctcc
      241 ggctgcgcca gcaaagtgtc gaggtcgcgc cgggcggcgg ccaggatgcc gtccatgccc
      301 gcggcggcgt tgtaagccgg cgtgccgggg aggggcggca ggttgacaat cgcgatcagc
      361 ggcttgccgg tgccggggaa gatgcgcgcg aagcgcctgc tcaggttctt catccgtcgc
      421 tccctccggc tcgggccgag tcgccccagg gcctgttggt ggatcactgc ttgcagactc
      481 ggtgccgtgc gcctttgcgt agacttgccg gaacggcccg ctccggccgc gggttgcaga
      541 gaggaaaatg cgtcatgagc cggctcacat gcttgtcggt gatcggcctg cttggcctcg
      601 gctcgctgct ggcggcctgt agcagcgggg gctcgccagc cagcccggcg cccagtgcgg
      661 cggccctcgc ctcgccgccc gcggccgcta cggctcccgc cacgacagcg gccacggctg
      721 ccccggcaag ccccaccgcc gtcgccaccg cgacagcggc cgaggccagc gccactcaac
      781 ctccgtctcc gaccgccacc gccgtgccga cgaccgcgcc cagctccacg ccgaccccgg
      841 ctccgaccgt gcccagcggg cccccccagg cgcaccaggt ggcgggcggc acgatcatgc
      901 tcccggccgg gttcaatatc ggcgtcttcg ccgaggactt gggcggcccg cgcttcatgg
      961 cggtgcggcc gagcgatggt ctgctcttcg ccaccgacat gcgcggcggg cgcatcctgg
     1021 tcctgccgga cgcgaaccac gacgggaaag ccgacagcgt gcaggtcttc gccagcgggc
     1081 tcaacctgcc gtcgagcctg gcgttctacc aggactggct ctacgtcggt gagacgaacc
     1141 aggtgtcgcg cttccgggcg ccgcagggcg cgctcaagcc cgagggcggc aaagaggtcg
     1201 tggtgccgaa cctgccctcc ggcggtgggc acttcacccg cacggtggcc ttcagccccg
     1261 acggccggct cttcatcagc gtcggctcct cctgcaacgt ctgcgtcgag tccgacaacc
     1321 gccgcgccgc tatcctggtg gccaacgccg acggcagcca gctccggctc tacgccaccg
     1381 ggctgcgcaa cgcggtcggg ctcgcgttcc agccgggcac gaatcagctc tgggccacgg
     1441 tcaacgggcg cgacaacctc ggcgacaacc tgccgcccga cgacctgcgc cgcatcgagg
     1501 atggggtctt ctacggctgg ccctactgct acgacggcga gcaccccaac cccgagttca
     1561 acgactccgg caaatgcgcc ggggtgccgc cggacaccgt cgcgctgccg gcgcactcgg
     1621 ccgcgctggg gctgaccttt gggcagggct tgcaggctcc ccagccctac caggacagca
     1681 tctacatcgc ctaccacggc tcctggaacc gctcgattcc gaccggctac aaggtggtgc
     1741 gggtgcccct ggtcaacggg cagccggggc agccggagga cttcgcctgg ggttggctgc
     1801 cgggctcggc ccagaacccg gggcaggtat ggggccgtcc ggttggcgtc accgtcggca
     1861 gcgacggcgc gctctacgtc agcgacgact cgaccgggct ggtctaccgg atctggttcg
     1921 gggggtagac ggactgccca tcgaagtggt aggcagcgcg tgggggggac ggatccttcg
     1981 actgcgctca ggatgacagg gtaagggaga ggcgaacgac gacaatgatc gtcttcgtca
     2041 gcccccgatt aaccctgtca tcctgagcgc agtcgaagga tccgacttgc ccgacgtacc
     2101 atccgtgtcg tcggccgcgg ttccgccccg acggcgcctt ttagctggtc aggaccgcct
     2161 cgcgcgcccg gtaggccgcc gggcggcggt tggccagcga ggggagctgg gcgcgcaact
     2221 gccgcaggta ggcgaagtcg agctcggcgg tgaccacgcc ctcgcggtcg ggcgcgtcgg
     2281 cgatgaccgt gccccaggga tcgaccacca ggctgtgccc gtagcagcgc gcgtgcggct
     2341 cgtgcggccc gatctgcgcc ggggcgatga cgtagcactg gttctcgatg gcgcgggcgc
     2401 gcagcagcgc gtgccagtgg tccttgccgg tgtagagggt gaaggcggcc ggtacgcaga
     2461 ggacctcggc gcccgccagc gccagcaggc ggtagagctc cgggaagcgc aggtcgtagc
     2521 agatggtcag gcccagcgtg tgcccgccgg cctcggcggt caccacgtcc tcgccgggga
     2581 ggatggtggc cgactcgttg gcgctgacgt tgccggtcag gtcgatgtcg aagaggtgga
     2641 tcttgcggta ggtggccagc atagcgccgt ccgggccgta gaagacgctg gtgttgtagt
     2701 acttgcccgg cacgtcgctg cgctcgtgga tcgagccgcc caggaggtag ataccgtgct
     2761 ggcgcgcttt ggcggcgaag cgcgccgtgg tcgggccggg gatcggctcg gccaccgcct
     2821 catgcgcctc ctttggtccc aggtaggcca cgtactcggg cagcgcgacc aggcgcgcgc
     2881 cattcccggc cgcctcgtcg atcagccgct cggctttggc gaggttggcc tccttgtcgg
     2941 cctgcgtgtt gatctgcacc agcccgaccc gcatcacgtc gctcattccc ggctcctttg
     3001 ctcgtgtcca tgcctatagc tccgattgta gcgcgaagcg tggcccgcgg cacgctggac
     3061 aagatttggc gggcgtgcta caagagggac tatcgtcaag ggggtttgta gcggggtcaa
     3121 ggaggtgtca tgcacgaggt cttcccggtc gcggcgggtc tgctggtggg cgccctcgcc
     3181 tatcgggttt cgtcgccgcg gctgcgcgcg ctcgtcatgg cggtgctcag cgtgctgttg
     3241 ggcgtggccg ccacgttcat cagcggcgag gcgctgatca gctgggggtt tgtcctgatc
     3301 gacatcccgc tggtactcgg cgcgagcgtg atcggttacg ggctgctggt gctctggcag
     3361 cgctggtcga cccaaccgcg ctagccactc accgcgacgg tccggcccgc tcgctgccga
     3421 gcgggccttt cccttgcccg cgcctactcc cactggaagg tgcgcgcggc gacgaccagc
     3481 gccagcagcg cccagccggc caggatcacc agattgctga gggggaaggt cgggtggctg
     3541 ccgagcgagg cgcgcagcgc gtcggcgaag gcgctggagg gcagcagccg cgctgggagc
     3601 gagaggagcc ccggcaggcg atcgaccggc cagacgatgc cgccgagcaa gaggaagagg
     3661 aggtagagcg cgttggcggc ggccagcgtc gtctcggcgc gcaccgcgcc ggccagcatc
     3721 agcccgaaga gcgagaaggt agcggtgccc aggatcatgg ccagcaccgc cagccagagc
     3781 gcgccgctgg ggtgccagtc gtagaaggcc caggagagcc cgatcagcag gacgatctcg
     3841 aacagctcga tcacgatcac cgagatgacc ttggcgccca ggagctgggc gcgcgtgagc
     3901 ggcgtgctgc ccaggcgctt gagcacgccg tagttgcgct cgtaggcggt gcggatcgcc
     3961 aggctgacca ggccggtgga catgaccgcc agcgccagca tgccgggcag gaggaagtcg
     4021 atagggcgct cgaacttgtc gggcttgagg ccgatggcgg cgaagaagac cagcagcacc
     4081 accggcacca ccatggtgat cagcacgccc tcgaagcggc gcaccgtcag catcagctcc
     4141 attcgcgtct gcgcccacaa ggcgctcatg cgtactcctt catcggatgg ccgtcgacga
     4201 tgttgggtgc taggtcagca ccgatccttc gactgcgctc aggatgacag ggggaggtaa
     4261 ggggcgtacg aggacgaacg gccatgctcg atcgcgccaa tccctcactc ccgcagctcg
     4321 tggccggtca ggcgcaggaa gacctcctcc agcgactcgt ggccgacgcg caggtcgttc
     4381 aacagcacgt cgcgctcgcg cgcccaggcg gtcacctcgg ccagcaggtc gatcggccgg
     4441 cgcgtctgga ccacgtagaa gccgggacgc acctcgcgcg cgccttcggt ggccggcagc
     4501 gcggccaggc cgccgacatc cagcccggcc gggccgctga actggacctc ctcgttgctg
     4561 gtggccgtca gtacgcgcgg ctcctccagc gccagcagct tgccgtggtc gatgatcgct
     4621 acgcggtcgg ccagccgctc ggcctcctcc atgaaatggg tcgtcagcag cacggtcagc
     4681 ccgcgcgccc gcagcgactg caggatgtcc caggtgacgt ggcgcgcctg cgggtccatg
     4741 gcggtggtcg gctcgtcgag gaagaggagc tccggcttgc cgatcagcgc ggcggccagc
     4801 gccaggcggc gctgctgccc gcccgagagc tggcggaagc gcacgtcggc cgactcctcc
     4861 agcccgacga gctgcaagag ggcgcgcggc tcctcggact cggggtagaa gtggcggaag
     4921 agctcgagga tctccagcgg ggtgacggtg gggtagatgc cggcgttctg cagcatcacg
     4981 ccgatgcgcc gcttcagttc gccgccctga cgctgcgggt cgagtccgag cacgcgcacc
     5041 tcgccgccgt cggccccgcg atagccttcc aggatctcga cggtcgtcgt cttgcccgcg
     5101 ccgttggggc cgaggagcgc gaagatctcg ccgcggcaga cgctgaagct gagtccgttg
     5161 accgcgcgct tgtcgccgta gctcttgacc aggtcgcgca cgacgatggc atcgtctctc
     5221 gcgctcgtcg ttcccccctc ctgcttcaag tccgcgtgaa gctgagctgg tagagcatca
     5281 tgtagatgac ccagccagtg acgaccacgt agagccagat cggcagcgcc acgcgggcca
     5341 gccgcttgtg gcgcgggaac tggcgcgtca gggcgcgata gagcacgacc agcacgatcg
     5401 gcacgatggc cgtggccagg atggtgtgcg tggtcaggat cgtcagatag acgacgcgca
     5461 ccgcgccgtg cccggcaaag agcttactgc cgtagagcgc cgagcgcagc aggtagacga
     5521 tcaggaagag ggcggcgaag ctggtcgccg tcagcatcga ccacttgtgt cgctggatct
     5581 cgccccggcg gatgaagcgg tagccgatca ggagcgcgac gccgctgatg agaatgaggc
     5641 tggtgtcgat gagcgggagc cagtgcgcca tgccggtggt cgtcgtccct ctagtggtcg
     5701 acccgaaacg cgaacccggt caccaattgt acgccccggc gcgcggcgcg gacgtcggga
     5761 gtctcccgca cggcgctcgc cgtcagcgcg gcgcggtcgg ccacccgccg tgctcttgac
     5821 tttcggcaca agcgcttcta ccctggcaat actgtcgtga ctgacttcaa gcttgctgct
     5881 gactctac
//
LOCUS       DASUBI010000769         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_666222, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000769 DASUBI010000000
VERSION     DASUBI010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666222"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="groEL"
                     /locus_tag="VFU72_15100"
     CDS             complement(<1..972)
                     /gene="groEL"
                     /locus_tag="VFU72_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5424871.1"
                     /translation="MAKVIEFNEDARKSLKQGIDTLANAVKTTLGPKGRNVALDKKFG
                     APTVTHDGVTVAKEIELEDPFANMGAQLLKEAATKTNDVAGDGTTTATVLAQAVVHEG
                     LRNVAAGANPMLLKQGIVLGTEQVVDYLKGQAKQIGGKADIAQVAAISAADQEIGDLI
                     ADVMEKVGKDGVITVEESRGLNFETEFTEGMQIDRGYISAYFVTNPERMEAELDEPYI
                     LITDKKISAVADILPVLEKALQVTKNFMIIAEDVDGEALATLVVNKLRGTINVLAVKA
                     PGFGDRRKEMLRDIATLTGGEVISEEVGRKLDSVQVEDLGSARRIVST"
     gene            complement(1029..1340)
                     /gene="groES"
                     /locus_tag="VFU72_15105"
     CDS             complement(1029..1340)
                     /gene="groES"
                     /locus_tag="VFU72_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5424872.1"
                     /translation="MANTSKSNGKVTPLGDRVLVQPLQREEVTRSGIVLPDTAKEKPQ
                     RGKVIAVGSGRLTEEGKHLPMDVKEGDEVLFAKYAGTELKIEEDEYLILSEKDILAKL
                     A"
     gene            complement(1535..>2139)
                     /locus_tag="VFU72_15110"
     CDS             complement(1535..>2139)
                     /locus_tag="VFU72_15110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5424873.1"
                     /translation="AARAAGTPFPELVRELVLDPAGLEQTFMPPPAAAYARVATVAGV
                     FAFGTDAAMYNSPYALALGHPAFGAVASVRDLLRFGLLFGPASGGHRILSEATIRAMT
                     TDQTGGEARDHLSGLRRPWGLGFSVRASASQLGLGDLTSPSAFGHPGASGCTLVIDPS
                     YDLALAYVSNQHGLSGWERWNARLDTVVNGVLAALTRRPG"
BASE COUNT          300 a          731 c          707 g          401 t
ORIGIN      
        1 ggtcgagacg atgcggcggg cgctgcccag atcctcgacc tggacgctgt cgagcttgcg
       61 gccgacctct tccgagatga cctcgccgcc ggtcagcgtc gcgatgtcgc gcagcatctc
      121 cttgcggcgg tcgccgaagc ccggcgcctt gaccgccagc acgttgatcg tgccgcgcag
      181 cttgttgacg accagggtgg cgagcgcctc gccgtcgaca tcctcggcga tgatcatgaa
      241 gttcttggtg acctggagcg ccttctccag cacgggcaga atgtccgcca cggccgagat
      301 cttcttgtcg gtgatcagga tgtacggctc atccagctcc gcctccatgc gctcggggtt
      361 ggtgacgaag taggcggaga tgtagccgcg gtcgatctgc atgccctcgg tgaactcggt
      421 ctcgaagttc aggccgcgcg actcctcgac ggtgatcacg ccgtccttgc cgaccttctc
      481 catgacgtcg gcgatcaggt cgccgatctc ctggtcggcg gccgagatgg cggccacctg
      541 ggcgatgtcg gccttgccgc cgatctgctt ggcctgaccc ttgagatagt cgacgacctg
      601 ctcggtaccc agcacgatgc cctgcttgag cagcatgggg ttggcgccgg cggccacgtt
      661 gcgcaggccc tcgtgcacga ccgcctgggc cagcacggtg gcggtggtgg tgccgtcacc
      721 ggcgacgtcg ttggtcttgg tggcggcttc cttgaggagc tgggcgccca tattggcgaa
      781 ggggtcttcc agctcgatct ccttggccac cgtcacgccg tcgtgggtca cggtcggggc
      841 gccgaacttc ttgtcgagcg ccacgttgcg gcccttgggg ccgagagtcg tcttgaccgc
      901 gttggccagc gtgtcgattc cctgcttgag cgatttgcgc gcgtcctcgt tgaactcgat
      961 gactttcgcc acgtctccat ccctccaccc ggccctcgat ggccgggata gctgtatcga
     1021 tcagcggact acgccagctt ggccagaatg tccttctcgc tgaggatcaa gtactcatct
     1081 tcctcgatct tcagctccgt gccggcgtac ttggcaaaga ggacctcgtc gccctccttg
     1141 acatccatcg gcaggtgctt gccctcctcg gtcagccggc cggagccaac cgcgatcact
     1201 ttgccgcgct gcggcttttc tttggcggtg tcgggcagga cgatgccgct gcgcgtcact
     1261 tcctcgcgct gcagcggctg caccagcacc cggtcgccca gtggtgtgac tttgccgttg
     1321 cttttggacg tattcgccat cctttgggaa cctccctttg cctgcggcgg gcctcccccg
     1381 cccttccgaa acctgagcac tatgttagca ctctctcgcc gtgagtgcta acagccgtat
     1441 catagccaac cgctcttgtc gctgcaagag ccagtcatga ttgtccccac aaatccgggg
     1501 ccatcgcgtt cgccgtgcgg cggatcaggc cggctcagcc cggccggcgc gtcagggcgg
     1561 ccagcacgcc gttgacgacc gtatccagcc gggcgttcca gcgttcccag ccgctgagcc
     1621 cgtgctggtt ggagacatag gccagcgcca gatcgtagct ggggtcgatc accagcgtgc
     1681 agccgctggc acccgggtgc ccgaaggcgg acggcgaggt caggtcgccg agccccagct
     1741 ggctagcgct ggcccgcacc gagaagccaa ggccccaggg acggcgcagg ccgctcaggt
     1801 ggtcgcgcgc ttcgcccccg gtttggtccg tcgtcatggc gcggatcgtc gcctcggaga
     1861 ggatgcggtg tccgccgctg gccggtccga agagcaggcc gaagcgcagc aggtcgcgca
     1921 ccgaggcgac ggcgccgaag gccgggtgcc ccagtgccag cgcgtagggc gagttgtaca
     1981 tggcggcgtc ggtgccgaag gcgaagactc cggccacggt cgccacgcgc gcgtacgccg
     2041 cggccggcgg cggcatgaag gtctgctcga gccccgccgg gtccagcacc agctcgcgca
     2101 ccagctccgg gaagggtgtt ccggccgcac gagcagcca
//
LOCUS       DASUBI010000770         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_775555, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000770 DASUBI010000000
VERSION     DASUBI010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775555"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFU72_15115"
     CDS             <1..1198
                     /locus_tag="VFU72_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5424874.1"
                     /translation="AGRDLSATHVAHGSTRVMKTCAVIGQASGTAAALAAHCGLTPRA
                     LAGDCAAVRAVQQTLLRAGAYLPLRRNEDEGDLARRPGVTVTATSEAALRLDNDDTAN
                     PSGWELAGLSFPEHYNPLAGQRPGGPAAIPLTVPIAQSFVVSAGRLDAVTLRLCSSAV
                     EPVTARLHIRQAAQLRDFGPCAGAGEALATLEATVLPGDTAVTFQAATPVTTTPGQPV
                     VLLLEPLPDVAWRLSAQEPPGTQAGRWDEELGYWRWLHGTLHVNLTPVSRPYAAVNVL
                     SGVTRPEVGANMWIADPAAPLPQALELAWPAPVTLGRVELTFDSQLSGWVWEGAFPLV
                     AREYRVDAVGPDGVWATLADVSGNWQRRRVHTCAPRAISRLRVTILATNGGRTARLVE
                     VRAYAE"
     gene            complement(1186..1674)
                     /locus_tag="VFU72_15120"
     CDS             complement(1186..1674)
                     /locus_tag="VFU72_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424875.1"
                     /translation="MNASASACGPFSGGSPNQNSIVTLPLVFSTVGEGKEPAAGLAAG
                     EAALVADAAAVELVALVAALVAALLLAALVLPLVAVAPPPEAGALVGAFVGAALAVVL
                     LLDVAVVAVGLLDPQAASNRVTLPPARPVSNCRRVSRWDGCRAAVMIPSYSVCPIPRH
                     SA"
     gene            1673..>2593
                     /locus_tag="VFU72_15125"
     CDS             1673..>2593
                     /locus_tag="VFU72_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5424876.1"
                     /translation="MKQYPGIKVNTTRYVNDDTGNTKLDTALQGGTPIDVYMSYGIPR
                     LGQRIRAGVAEELTSYIAGDPAVKAWTETTDGLFKYQGKFFSLPTVRDPYAVIINKKL
                     LEAAGGKLPTQWTIDDFHTLARQVSSKDVFGCYAPPDLARQIIGPDYWYKNGGKESNF
                     DNPAFKQYLDLYTGMIKEKSAFPWTEVLAQNLRVYQQNVYLTSQAVLWPGSMYVLRYV
                     NDKKQYPHDFVTTFAPMPTPTGSGKFYNPGSISNDVILNPKAKNKEAAWAFFRFRLTD
                     GAKYMLKSGKVPAFPGASPDEVIDGILGPDK"
BASE COUNT          443 a          917 c          861 g          372 t
ORIGIN      
        1 cgcgggacgc gacctcagcg ccacgcacgt cgcgcacggc tccacgcgcg tgatgaagac
       61 ctgcgccgtc atcggccagg ccagcggcac ggcggcggcg ctcgccgcgc actgcggcct
      121 cacgccccgc gcgctcgccg gcgattgtgc ggcggttcgc gcggtgcagc agacgctctt
      181 gcgcgccggc gcctacctac cgctgcgccg caacgaggac gagggtgacc tcgcccggcg
      241 gcccggcgtc acggtcacgg cgacgagcga agcggcgctg cgcctggaca acgacgatac
      301 agccaatccg tctggctggg aactggccgg gctgagtttc cccgagcatt acaatccact
      361 cgccgggcag cggcctggcg gacccgccgc gatccctctg accgtgccga tcgcacagtc
      421 cttcgtggtc tcggcggggc ggctcgacgc ggtcacgctg cggctctgct ccagcgccgt
      481 cgagccggtg acggcgcgac tgcatatccg gcaggccgcg cagctgcgcg atttcggccc
      541 gtgtgctggc gccggtgagg cgctcgcgac gctcgaagcg actgtgctgc ccggcgatac
      601 ggccgtcacg ttccaggccg caacgccggt gacgaccacg ccggggcagc cggtggtcct
      661 gctgctcgaa ccgctgccgg acgtcgcctg gcgtctctct gcgcaggagc cgcccggcac
      721 gcaggccggg cgctgggacg aggaacttgg ctactggcgc tggctgcacg gcaccctgca
      781 cgtcaacctg acgccggtca gccggccgta cgcggcggtg aacgttctca gtggcgtcac
      841 ccggccggaa gtcggcgcga atatgtggat tgcggacccc gccgcgccgc tgccccaggc
      901 gctcgaactc gcctggcccg cgccggtgac gctgggccgc gtcgaactga ccttcgattc
      961 gcaattgagt ggctgggtct gggaaggcgc tttcccgctc gtcgcacgcg agtatcgcgt
     1021 tgacgcggtg gggccagacg gcgtctgggc cacactcgcg gatgtcagcg gcaactggca
     1081 gcggcgtcgc gtccatacct gcgcgccgcg cgcgatcagc cggctgcggg tcaccatcct
     1141 ggcgacgaac ggcgggcgca cggcccgcct ggtcgaagtg cgtgcctacg cggagtgacg
     1201 cggtatcgga cagacagagt aggaggggat catgacggcg gcacggcatc catcccaccg
     1261 gctgacgcgc cggcagttac tcaccggcct ggcgggcggc agtgtcaccc tgttgctcgc
     1321 ggcctgcgga tcgagcagtc ccacggctac caccgccacg tcgagcagca gcacgacggc
     1381 gagcgccgcg ccgacgaaag cgccgacgag cgcgccggcc tctggtggcg gcgcgaccgc
     1441 gacaagtggc agcacgagcg cagctagcag cagcgcggcg accagcgcgg caaccagcgc
     1501 gacgagttct accgctgcgg cgtccgcgac caaagcggcc tcgccggcgg ccagccccgc
     1561 cgccggttcc ttcccttcgc ctacggtgct gaagacgagc ggcaatgtaa cgatcgagtt
     1621 ctggttcggc gagccgcccg aaaatgggcc gcaggcgctc gccgatgcgt tcatgaagca
     1681 gtatccaggc atcaaggtca acacgacccg ctacgtcaat gacgacaccg gcaacaccaa
     1741 actggacacc gccctgcaag gtggcacgcc gattgacgtc tacatgtcgt acggcatccc
     1801 ccgcctgggc cagcgcatcc gggcgggcgt ggcggaagaa ttgacctcct acatcgcggg
     1861 tgacccggcg gtcaaggcgt ggaccgagac gaccgacggc ctcttcaagt accagggcaa
     1921 gttcttcagc ctgccgaccg tgcgcgatcc ctatgccgtc attatcaaca agaagttgct
     1981 ggaggcggcc ggcggcaaac tgccgaccca gtggaccata gacgacttcc acacgctcgc
     2041 caggcaggtc tccagcaagg atgtcttcgg ctgctacgcg ccgcccgacc ttgcacggca
     2101 gattatcggg ccggactact ggtataagaa tggcggcaag gagtcgaact tcgacaatcc
     2161 cgcgttcaag caatacctcg acctctacac cggcatgatc aaggagaaga gcgccttccc
     2221 ctggaccgag gtgctggcgc agaacctccg tgtctaccag cagaatgtct acctgaccag
     2281 ccaggcggtc ctctggcccg gctcgatgta cgtcttgcgc tacgtcaacg acaagaagca
     2341 gtacccgcac gacttcgtca ccaccttcgc gccgatgccg acgccgaccg ggtcgggcaa
     2401 gttctacaat ccgggcagca tcagcaacga cgtcatcctg aaccccaagg cgaagaacaa
     2461 ggaggcggcc tgggccttct tccgcttccg cctcaccgat ggcgcgaagt acatgctcaa
     2521 gagcggcaag gtgccggcct tccccggcgc cagccccgac gaggtgattg acggcatcct
     2581 ggggccggac aag
//
LOCUS       DASUBI010000771         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_203641, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000771 DASUBI010000000
VERSION     DASUBI010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203641"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="cbiM"
                     /locus_tag="VFU72_15130"
     CDS             <1..1021
                     /gene="cbiM"
                     /locus_tag="VFU72_15130"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HEU5424877.1"
                     /translation="VLYGAAAPFWWRASRVVKRLLTGQTVPLLALFSAFCFTVMMFNI
                     PVPGGTTAHAVGGTLAAIVLGPWPAIIAVSVALIIQAIFFGDGGITAIGANCFNMAIV
                     LPLVGYGVYKLLARNAAVPSRRRVVAAAIGSYVGINAAGLLVGIELGIQPIFWSQNGH
                     ALYAPYSLSVAVPAMMIPHLTLAGAAEAIATAFGVAYVQRVQPQLLLQRVGGAGGEAR
                     APARGRVAITAAIAVLMLVAVPLGLLASGSAWGEWNPEQLGGNVGYVPSGLQRLHGVW
                     SAPLSGYSLPWIPHEAPFAELAVGYILSAVVGAGLILIAAFAVRILARRRAEAHASSA
                     GGAGG"
     gene            1023..1907
                     /gene="cbiQ"
                     /locus_tag="VFU72_15135"
     CDS             1023..1907
                     /gene="cbiQ"
                     /locus_tag="VFU72_15135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEU5424878.1"
                     /translation="MTAEAKPARARSRRRDRRDFVAKTIGGVTEAVERAVYTEEHARR
                     PGMLQRVDPRAKLGVFLAAVLVVGLAHNLWIILALYALTLALGLSSRIPADLLLKRVL
                     LGIPLFAGIVALPALLLGHGQPLLEIGRLGPLHLVITRASLLGFVIFVARVAASVTLA
                     ALLVVTTRWADLLKALRAMRVPEAFVVVLGMTYRYIFLFLRALENLLLARASRTVGAA
                     REQEARRWVGASVGTLLGKSLRTSNEVYQAMVARGYSGDVRTLSSFQMRDEDWLFTLS
                     ALVLLAVALLIDLSLRWR"
     gene            1907..2725
                     /locus_tag="VFU72_15140"
     CDS             1907..2725
                     /locus_tag="VFU72_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424879.1"
                     /translation="MEYAPAFEVEAVSFSYHGRQPALQQVDLTVDQGEQLVILGANGS
                     GKSTLLKVLDGLYRPDSGRIRAFGEDVTDVAEDPPRSRALHRRVGLVFQDPDVQLFCP
                     TVYDDVAFGPLQLGWPEEQVRAAVERALEQMDVTHLADRAPYELSGGEKKRVAIATVL
                     SMEPEAILLDEPTASLDPRSSDALLDVILGLRRLGKTVVLTTHELEIVPLLATRVVVF
                     GDQERRPVASGPAQDILADEELLVRTNLIHEHLHHHGTYVHSHYHDATHHDRDD"
BASE COUNT          364 a         1007 c         1012 g          477 t
ORIGIN      
        1 ggtgctctac ggcgcggcgg cgcccttctg gtggcgcgcc agccgggtgg tcaagcgcct
       61 cttgaccggg cagacggtgc cgctgctggc tctcttctcc gccttctgct tcacggtgat
      121 gatgttcaac atcccggtgc cgggtggcac gaccgcgcac gcggtgggtg gcacgctggc
      181 ggccatcgtg ctgggcccct ggccggcgat catcgccgtc tcggtggcgc tgatcatcca
      241 ggcgatcttc ttcggcgacg gcggcatcac cgccatcggc gccaactgct tcaacatggc
      301 gatcgtgctg ccgctggtgg gctatggcgt ctacaagctc ctggcgcgca acgccgccgt
      361 gccgtcgcgc cggcgcgtcg tcgcggcggc catcggcagc tacgtcggca tcaacgccgc
      421 cggattgctg gtgggcatcg agctgggcat ccagccgatc ttctggtcgc agaacggcca
      481 cgccctctac gcgccctact cgctcagcgt ggccgtgccg gccatgatga tcccccacct
      541 gacgctggcc ggggccgccg aggcgatcgc caccgccttc ggggtggcct acgtgcagcg
      601 ggtgcagccg caactgctgc tgcagcgcgt gggcggcgcg ggcggggagg cacgcgcacc
      661 ggcccgtggc cgggtggcca tcaccgccgc catcgcggtg ctgatgctgg tggccgtgcc
      721 gctggggctg ctggccagcg gctcggcctg gggcgagtgg aacccggagc agctgggggg
      781 caacgtgggc tacgtcccca gcgggctgca gcggctgcac ggagtctggt cggcgccgct
      841 ctccggctac tcccttccct ggatcccgca cgaggcaccc ttcgccgagc tggcggtcgg
      901 ctacatcctc tcggcggtgg tcggcgcggg gttgatcctg atcgcggcct tcgcggtgcg
      961 tatcctggca cgccggcgcg ctgaggcaca cgcctcgtcg gcgggtgggg ctggaggttg
     1021 agatgacggc tgaggcgaaa cctgcccgcg cccgctcccg gcgccgcgac cgccgggact
     1081 tcgtggccaa gaccatcggc ggcgtcaccg aggcggtgga gcgcgcggtt tacaccgagg
     1141 agcatgcgcg tcgccccgga atgttgcagc gggtcgaccc gcgcgccaag ctcggcgtct
     1201 tcctggcggc cgtgctggtg gtcgggctgg cgcacaacct ctggatcatc ctggcgctct
     1261 acgccctcac gctggcgctg gggctcagct cgcgcatccc ggccgatctc ttgctcaagc
     1321 gggtgctgct cggcatcccg ctcttcgccg gcatcgtggc cctgccggcg ctcttgctgg
     1381 gccacggcca gccgctgctg gagatcggcc ggctcggtcc gctgcacctg gtgatcacgc
     1441 gcgcctcgct gctcggcttc gtcatcttcg tcgcgcgcgt ggccgcctcg gtgacgctgg
     1501 ccgcgctgct ggtggtgacg acgcgctggg ccgacctgct caaggcgcta cgcgccatgc
     1561 gcgtgcccga ggccttcgtg gtggtactcg gcatgaccta ccgctacatc ttcctcttcc
     1621 tacgcgcgct ggagaacctg ctgctggcgc gggccagccg cacggtcggc gcggcgcgcg
     1681 agcaggaggc gcgccgctgg gtgggcgcca gcgtgggcac gctcttgggc aagagcctgc
     1741 gcaccagcaa cgaggtctat caggcgatgg tcgcgcgcgg ctactccggc gatgtccgta
     1801 cactctccag cttccagatg cgcgatgagg actggctctt caccctgtcg gcgttggtgc
     1861 tgttagcggt ggccctgttg atcgatctca gtttgaggtg gcgctagtgg aatacgctcc
     1921 cgcgttcgaa gtcgaggctg tctcgttcag ctaccacggg cgccagccgg cgctgcagca
     1981 ggtcgatctg accgtggacc agggggagca actggtcatc ctgggggcca acggcagcgg
     2041 caagtcgacc ctgctcaagg tgctcgacgg cctctaccgg ccggactcgg gccgcatccg
     2101 cgccttcggc gaggacgtca ccgacgtggc cgaggacccg ccgcgctccc gggcgctgca
     2161 tcgccgggtc gggctcgtct tccaggaccc cgacgtacag ctcttctgcc ccaccgtcta
     2221 cgacgacgtg gccttcggtc cgctgcagct cggctggccc gaggagcagg tgcgcgcggc
     2281 ggtggagcgg gcgctggagc agatggacgt gacgcatctc gccgaccggg ccccctacga
     2341 actgagcggc ggcgagaaga agcgggtggc gatcgcgacc gtgctctcga tggagccgga
     2401 ggcgatcctg ctggacgaac cgaccgccag cctcgacccg cgcagcagcg acgcgctgct
     2461 ggacgtcatc ctgggactgc ggcggctggg caagaccgtc gtgctgacga cgcacgagtt
     2521 ggagatcgtg ccgctcttgg cgacgcgggt ggtggtcttc ggcgatcagg agcggcgccc
     2581 ggtggcctcg ggcccggcgc aggacatcct ggccgacgag gagctgctgg tgcgcaccaa
     2641 cctgatccac gagcacctgc accaccacgg gacgtacgta cactcccact accacgacgc
     2701 cacccaccac gaccgcgacg actgaacggc catagtgtac gtacatccgt ggtccaatta
     2761 gagcgcagag ctaacgcggt aggggcggac ctccgtgtcc gcccttgttg ccctatggcc
     2821 ggtcctcatc ggagccaccc gcgctccccc atttccccgt
//
LOCUS       DASUBI010000772         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_766829, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000772 DASUBI010000000
VERSION     DASUBI010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766829"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..655)
                     /locus_tag="VFU72_15145"
     CDS             complement(329..655)
                     /locus_tag="VFU72_15145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5424880.1"
                     /translation="MSELQDNLDEFRRAGAQVIGLSADAYHAQNAFAVQRDLEFPMLS
                     DFNKEAMAAFDITMADSGGYHGVARRSIFILQPDGTVAYADVPTERGVRPDVPAAVAK
                     AQQMAG"
     gene            complement(665..802)
                     /locus_tag="VFU72_15150"
     CDS             complement(665..802)
                     /locus_tag="VFU72_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424881.1"
                     /translation="MAIEVGAQVPEFTVLGAQNAKTTLKDILAQGPAVLAFYFFAFTG
                     G"
     gene            923..2122
                     /gene="cysS"
                     /locus_tag="VFU72_15155"
     CDS             923..2122
                     /gene="cysS"
                     /locus_tag="VFU72_15155"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5424882.1"
                     /translation="MRLYNTLSGQKELFTPLDGYTVRMYVCGVTPYDTTHMGHAMTYI
                     TFDVINRYCQYLGWRVRYVQNVTDIDEDILRKAKELGEQWDRLGDRYIFQFQEDLNAL
                     NVLPPAVYPRATGEIPMIVEVVQSLIEQGKAYEREGNVYFRVDRFDNYGQLCRLPDDE
                     MIRLSGERGANPNDPLKENPLDFILWQAAQPGQPSWESPWGPGIPGWHIECSAMAMKY
                     LGPVIDIHGGGGDLVFPHHECEIAQSESYTGEAPFSRFWVHVGMVRYEGEKMSKSLGN
                     LVLARDVLRYHSADAIRLYLLSHHYRDAWEYHNDGPARFEPLVATLREAALLRGGSGA
                     PLNVAPLKERLLAAVADDLDTPKAIEALRDLGQAITGAAEAGQDIEEARVALRTAGAV
                     LGLHVTR"
     gene            complement(2266..>3202)
                     /locus_tag="VFU72_15160"
     CDS             complement(2266..>3202)
                     /locus_tag="VFU72_15160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5424883.1"
                     /translation="HPAPSMATNPFVLRQGVRMKIGLVLPQTEIGDDPAVVRDYAQTA
                     EDLGYSHILAYDHIVGADVSNRPNWQGAYQLKDQFHEPFVLFSYFAAFTTRLELVTGV
                     IILPQRQTVLLAKQAAALDVLSGGRLRLGVGVGWNEVEYTALNENFKNRGRREEEQIE
                     VMRLLWTQPSVTFEGRWHHLPGVGINPLPVQRPIPVWLGGRSEPAYQRIARIADGWLP
                     QFRPDAQGVAIVEHVRELTRAAGRDPDQLGMESRLSLAQAPREQWRADLERWQALGAD
                     YLSINTMGMGLSPREHIATIERVYRELGVAEFNRG"
BASE COUNT          521 a         1109 c         1051 g          521 t
ORIGIN      
        1 ggccccgtgt cgcgataggt cacgttcagc tcgacgacgt gcaggtccat gcggatcctc
       61 ctcaggcgtc taatgatgcg aggcaccgaa cgcggtgctc tttcctcgga agatccgggc
      121 tagtgaggac ggatccttcg actgcgctca gagcctgccc tgagcctgcc gaagggatga
      181 cagggtaagt agcgggctga cgaatacgat ctgtgtcgcc cggcaggccg catccctttc
      241 cctgtcatcc tgagcctgtc gaaggatccg tcctcacgca gcacccaggc attccgatgg
      301 gcaccgccct tagcttgctc tccgtcgctt aaccggccat ctgctgcgcc ttggccacgg
      361 cggcgggcac gtccgggcgc acgccgcgct cggtcggcac gtccgcatag gccaccgtgc
      421 cgtccggctg aagaatgaag atcgagcgcc gcgccacgcc gtggtagccg ccgctatccg
      481 ccatcgtgat atcgaacgcg gccatcgcct ccttgttgaa gtcggagagc atcgggaact
      541 ccagatcccg ctgcaccgcg aaggcattct gcgcgtgata ggcatcggcg ctgagaccga
      601 ttacctgggc gccggctcga cgaaactcgt cgaggttgtc ctgcagctcg gacaacatgt
      661 cgcctcagcc accggtgaag gcgaagaagt agaaggcgag gaccgccggc ccctgtgcca
      721 ggatgtcctt cagcgtcgtc ttagcgttct gcgcccccag cacggtaaac tccggcacct
      781 gggcgccaac ctcgatggcc atctcgtgct cctctcaggc caaccaaact cgtccgcatt
      841 gtagcgggcg atctagtggt agcataggct ccgctggtgc gcgtgccggc atgggagtgg
      901 tgggagcgca ggaggaggcg gcgtgcggct ctacaatacc ctgagcggac agaaagaact
      961 cttcaccccg ctggacggct acaccgtccg catgtacgtc tgcggcgtca ccccctacga
     1021 caccacccac atgggccacg cgatgaccta catcaccttc gacgtcatca accgctactg
     1081 ccagtacctc ggctggcgcg tgcgctacgt gcagaacgtg accgacatcg acgaggacat
     1141 cttgcgcaag gccaaggagc tcggcgagca gtgggaccgc ctgggcgacc gctacatctt
     1201 ccaattccag gaggacctga acgcgctcaa cgtcctgccg ccggccgtct acccccgcgc
     1261 caccggtgag atcccgatga tcgtcgaggt cgtgcagtcg ctgatcgagc agggcaaagc
     1321 ctacgagcgc gagggcaacg tctacttccg cgtcgaccgc ttcgacaact acggccagct
     1381 ctgtcgcctg cctgatgacg agatgatccg gctctccggc gagcgcggcg ccaaccccaa
     1441 cgacccgctc aaggagaacc cgctcgactt catcctctgg caggccgctc agccgggaca
     1501 gccctcgtgg gagagccctt gggggccggg catccccggc tggcacatcg agtgcagcgc
     1561 catggcgatg aagtacctcg gcccggtgat cgacatccac ggcggcggcg gcgatctggt
     1621 cttcccgcac cacgagtgcg agatcgccca gagcgagtcc tacaccggcg aggcgccctt
     1681 ctcgcgcttc tgggtccacg tcggcatggt gcgttacgaa ggtgagaaga tgagcaagtc
     1741 gctgggcaac ctggtgctgg cgcgcgacgt gctgcgctac cacagcgccg acgccatccg
     1801 cctctacctg ctcagccacc actatcgcga cgcctgggag taccacaacg acggcccggc
     1861 gcgcttcgag ccgctggtgg ccaccctgcg tgaggcggcg ctgctgcgcg gcggctccgg
     1921 cgccccgctc aatgtcgccc cgctcaagga gcggctgctc gccgcggtag ccgacgacct
     1981 ggacaccccg aaggcgatcg aggcgctgcg cgacttgggc caggcgatca ccggcgcggc
     2041 cgaggcgggc caggacatcg aagaggcccg cgtcgcgctg cgcaccgccg gcgccgtcct
     2101 cggcctgcac gtcacgcgct gacgggccct catcccccgc cccttctccc aatgctggga
     2161 gaaggggagt agctacaggg gcatccagat gttcctcctg catcctcccc tctcccagca
     2221 ttgggagagg ggcggggggt gagggctccc cgttggggct gagggctacc cccggttgaa
     2281 ctccgccacg cccagctcgc ggtagacccg ctcgatggtg gcgatgtgct cgcgcggcga
     2341 gaggcccata cccatagtgt tgatcgagag gtagtcggcg cccagcgcct gccagcgctc
     2401 caggtcggcg cgccactgct cgcgtggcgc ctgggccagc gacaagcggc tctccatgcc
     2461 gagctggtct gggtcgcggc ccgccgcgcg cgtcagctcg cgcacgtgct cgacgatcgc
     2521 cacgccctgc gcatccggcc ggaactgcgg cagccagccg tcggcgatgc gcgcgatgcg
     2581 ctggtaggcc ggctcgctgc ggccgccgag ccagaccggg atcggccgct gcaccggcag
     2641 cgggttaatg ccgacgccgg gcaggtggtg ccagcgcccc tcgaaggtga ccgagggctg
     2701 ggtccagagc agacgcatca cctcgatctg ctcctcctca cgccggcccc ggttcttgaa
     2761 gttctcgttg agcgcggtgt attcgacctc gttccagccg acgccgacgc ccagtcgcag
     2821 ccgcccgccc gagaggacgt ccagcgcggc cgcctgcttg gccagcagca ccgtctgccg
     2881 ctgcggcagg ataatcacgc cggtgaccag ttccagccgg gtcgtgaagg cggcgaagta
     2941 gctgaagagg acgaagggct cgtggaactg gtccttgagc tggtacgcgc cctgccagtt
     3001 ggggcggttg ctgacgtcgg cgccgacgat gtggtcgtag gccagaatgt ggctgtagcc
     3061 gagatcttcg gctgtttggg cgtagtcgcg gaccaccgcg gggtcgtcgc cgatctccgt
     3121 ctgcggcagc accaggccga tcttcatgcg cactccttgt ctgagaacga acgggttggt
     3181 ggccatcgag ggtgctgggt gc
//
LOCUS       DASUBI010000773         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_170530, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000773 DASUBI010000000
VERSION     DASUBI010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170530"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFU72_15165"
     CDS             complement(<1..621)
                     /locus_tag="VFU72_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5424884.1"
                     /translation="MAAGRGGMTPKPDLLVVGAGPAGLALALQARAHGARVRLIERRR
                     DAFRPSRALILHPRTLEALRPLGVTDALLARADRAPAAHLHLGARMVPASLAELALPD
                     TAFPHLTLIRQLEVETVLARALKERGVAVERGVELLDLRDEGSGVRATLRTRSGVEEA
                     AFALVAGCDGPESTVRGIAGIGWEGGAYREEAVLADVELAGELVEDV"
     gene            complement(597..1271)
                     /locus_tag="VFU72_15170"
     CDS             complement(597..1271)
                     /locus_tag="VFU72_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424885.1"
                     /translation="MVRHGYIEESPLAGPARSLRSPGEGQPDQEGRGARIFLDRPPLP
                     YRKAAGAVVRCVVRTASLLARRRIHQPAKHLGLRLRFANGTSARVYRETVVTGEPPTD
                     PCVLLVCFRLHGVRGFGHTLFRWESLLNTPLFVGFPGFVAKLWLAHDQHDVYRGVYQW
                     DGPERADRYARALWRILRLGCAPESIHYVVLPGLRRDDVLATPGLLDAALPEEARAWW
                     RLVEAA"
     gene            1709..>2185
                     /locus_tag="VFU72_15175"
     CDS             1709..>2185
                     /locus_tag="VFU72_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424886.1"
                     /translation="MKTWLKLTCIPSCAAAVYALGVRPRLLRWGATDAEIAEPYPGAE
                     LIPEGERSATMAVTIDAPPARVWPWLAQMGYGRAGWYSWDHLDNLGRPSAAELHPEWQ
                     GIQVGDFLGPQEGMTSRAWEVAALEPERFLGLRASFDLRKGAWYDPAGPRPPFSTDS"
BASE COUNT          329 a          798 c          771 g          287 t
ORIGIN      
        1 cacgtcttcg accagctcgc cggccagctc gacatccgcc agcacggcct cctcgcggta
       61 ggcgccgccc tcccagccga tgccggcgat cccgcgtacc gtgctctccg gcccgtcgca
      121 gccggctacg agcgcgaagg cagcttcttc aacgccggac cgcgtgcgga gtgtcgcgcg
      181 tacgccggag ccttcgtcgc gcaggtcgag cagctcgacg cctcgctcca ccgcgacccc
      241 gcgctccttg agcgcccgcg ccagcaccgt ctcgacctcc agctggcgga tcagcgtcag
      301 atgcgggaag gcggtatcgg gcagcgccag ctcggccagg ctggccggga ccatgcgggc
      361 gccgagatgc aggtgtgccg cgggggcgcg gtcggcgcgt gccagcagcg cgtcggttac
      421 cccgagcggg cgcagcgcct caagcgtgcg cgggtgcagg atgagcgcgc gggaggggcg
      481 aaaggcatcc cgccgccgct cgatcagcct gacccgcgcg ccgtgcgccc gcgcctgcag
      541 ggccagcgcg agaccggccg gccccgcgcc caccaccagc aggtccggct tcggcgtcat
      601 gccgcctcga ccagccgcca ccaggctcgc gcctcctccg gcaaggcggc atcgagcagg
      661 cccggcgtgg cgagcacgtc gtcgcggcgc aggccgggca gcacgacgta gtggatcgac
      721 tcgggcgcac agcccagccg cagaatccgc cacagggcgc gggcgtagcg gtcggcgcgc
      781 tcgggaccgt cccactggta gaccccgcga tagacgtcat gctggtcgtg cgccagccag
      841 agcttggcga cgaagccggg gaagcccacg aagaggggcg tgttgagcag gctctcccag
      901 cggaagaggg tatggccgaa accgcgcacc ccgtgcaagc ggaagcagac cagcagcaca
      961 cagggatcgg tgggcggttc cccggtgacg accgtctcgc ggtagacgcg ggccgaggtg
     1021 ccgttggcga agcgcaaccg caggccgagg tgcttggccg gctgatggat gcgccgccgc
     1081 gccagcaagc tcgccgtgcg gacaacgcag cgcacgaccg cgccagccgc tttccggtag
     1141 ggcagcggcg gccggtcaag gaagattcgc gcgccccgac cctcctgatc cggctgccct
     1201 tcgccgggac ttcgaagcga cctggccggc ccggcaagcg gcgactcttc gatgtatccg
     1261 tgcctgacca tggagaactc cttcatcatc gtcgatggcc ccagatcacg ccccgacgaa
     1321 ccaggccgtg aaccccggaa tccattcgcc tccagtgtgc acccggcgcc gtgtcaacgc
     1381 cggtgacgaa ccgcgctggc atgtgacagt cgtgtagcaa tgcgccactg accgtgcgtc
     1441 cacgacgcca tctccggtcc gcacgccgcc gtttcacgaa cttgatacgc gccgctcgcg
     1501 gcttcgtacg gctttgtcac gcgcacggcg gtagcgtagt cagcgggagg ccggcgacgg
     1561 tcggcccagc caatgcgcgc cgctcgcgcc cttcgcgccg gccagcccac cgggcgcggc
     1621 gcgcgccttc atacgagcgc tcgcgtcccc ttccagagcg cgagtgacgt ggaaacggcc
     1681 tttccgccac acgagagagg agtgcgacat gaagacgtgg ctcaagctca cgtgcatccc
     1741 cagttgcgcg gcggcggtct atgccctggg tgtgcgcccg cggctcctgc gctggggcgc
     1801 cacggacgcg gagatcgcgg agccctatcc gggagccgag ttgatccccg agggcgaacg
     1861 ctcggcgacc atggcggtga cgatcgacgc gccacccgcg cgggtctggc cctggctggc
     1921 gcagatgggc tacgggcgcg cgggctggta ctcctgggac catctcgaca atctgggccg
     1981 gcccagcgcc gcggagctcc atccggagtg gcagggcatt caggtgggcg acttcctggg
     2041 tccgcaagag ggcatgacca gccgcgcctg ggaggtggcg gcgctggagc cggagcgatt
     2101 cctggggctg cgcgcctcgt tcgatctccg caaaggggcc tggtacgacc ccgcgggacc
     2161 caggccgccg ttctctaccg actca
//
LOCUS       DASUBI010000774         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1299409, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000774 DASUBI010000000
VERSION     DASUBI010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299409"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFU72_15180"
     CDS             complement(<1..270)
                     /locus_tag="VFU72_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424887.1"
                     /translation="MSRRPARQETADENRLRNAADVDAVILKIELLGGFRAVVREREL
                     GARDWRSRRASRVVKLLALAPGHALHREQLCDQLWPEADPEDAANN"
     gene            574..873
                     /locus_tag="VFU72_15185"
     CDS             574..873
                     /locus_tag="VFU72_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424888.1"
                     /translation="MEEQKASLADVLLRCPTLDVGAAVDDFRQRVNEAVQRVDPAASV
                     VVELRTDWIAIEPQAGSHDPDVRDAMLATLYAVKERWFRELREASEQGAGDSGMR"
     gene            complement(1019..1855)
                     /locus_tag="VFU72_15190"
     CDS             complement(1019..1855)
                     /locus_tag="VFU72_15190"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5424889.1"
                     /translation="MSGSIAAELLVLRKRVAPWVLLGIWLALALTFAYLLPYLTYKGR
                     ADFGNGPSLAPLLPQGLVGSLIGGFPFYGGTLALMLGVLSLGSDFGWDTFKTLFTQGP
                     SRLRVFRAKLIALGIALIPFVLLVYGLGALSALLIAQVEHAPIAWPDAGLLARALLAS
                     WFILAVWAAFGVLLAVLTRGTALAIGLGILWILVIEGLVGALFDQSSLLRPLVKFFIR
                     ANAYSLGQPLGAAAGSAASGGPGTFAGPFVSAEQALLVLACYFVALLLLAGLLLRRRD
                     VA"
     gene            complement(1852..>2117)
                     /locus_tag="VFU72_15195"
     CDS             complement(1852..>2117)
                     /locus_tag="VFU72_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424890.1"
                     /translation="GRDHLRLRASPLETARRVVLALPEVEDVTPLDDTLRIAAGPGAA
                     RVIARSLVEAGVALDELYREQNSLEQVFLELTRETVEGEGEDA"
BASE COUNT          348 a          720 c          768 g          281 t
ORIGIN      
        1 gttgttagcg gcgtcctcgg gatcggcctc gggccagagc tgatcgcaga gctgctcccg
       61 gtgcagggcg tggccgggcg cgagagccag cagtttgacc acgcgcgacg cccggcggct
      121 gcgccagtcc cgcgcgccca actcgcgctc gcggacgacc gcgcggaacc cgcctagcag
      181 ctcgatcttg agtatgacag cgtccacgtc cgcggcgttg cgcaagcggt tctcgtcggc
      241 ggtctcctgc cgcgctgggc ggcgactcat ttgttccacg caccactcag agtgacggcg
      301 gcacgacggt cctggccagg gtgaccgggc ggccgagcca acagcgacga agggcctgac
      361 agtcgctatt gtacgcagta ttccgtcggt tgtcgataca tctgcatagc gcgtgcgtac
      421 taatcccgcg ttcgcgggag attaagcgcc gaaatccggc agcgacagcg gtcgaagtgg
      481 gttcggagcg acgcctggag cgcgcggcag gccgcggtcg ggctcttgcg aagcggcgcg
      541 gcgtggtcgg tcggcttcgg aggggtggcg aagatggaag agcagaaggc gtcgctggcg
      601 gatgtgctgt tgcgctgccc cacgctcgac gtgggggcgg cggtggacga cttccggcag
      661 cgggtgaatg aggcggtgca acgggtcgat cccgctgcca gcgtggtggt cgagctgcgc
      721 accgactgga tcgcgatcga accccaggcg ggatcacacg atccggacgt gcgcgacgcc
      781 atgctggcca cgctctacgc ggtcaaggag cgctggttcc gcgagctgcg cgaggcgtcg
      841 gagcagggcg cgggcgactc ggggatgcgt tgagcgccgg gctatctccg tcccggcgct
      901 gcggctcact cgttggacga ggacgggtag acgcgatcgg tgggggctga gggccattgc
      961 cgccagcccc gccctgatgg ttctgagcgt cgcggagtca ccgtgacggg cgcgcggatc
     1021 acgccacgtc gcgccggcgc agcaggagcc cggccagcag caacaaggcg acgaagtagc
     1081 aggccagcac cagcagcgcc tgctcggcgc tgacgaaggg tccggcgaag gtgcccggtc
     1141 cgccgctggc cgccgagccc gccgccgcgc ccagcggctg gcccagcgag taggcgttgg
     1201 cgcggatgaa gaacttgacc agcggccgca gcaggctcga ctggtcgaag agcgcgccga
     1261 ccagcccctc gatcaccagg atccagagga tgcccagccc gatcgccagc gcggtaccgc
     1321 gcgtcagcac ggctagcagc acgccgaagg ccgcccaaac cgccaggatg aaccagctcg
     1381 ccagcagcgc gcgtgcgagc agcccggcgt ccggccaggc gatcggcgcg tgctcgacct
     1441 gagcgatcag cagggcgctg agcgcgccga ggccgtagac cagcagcacg aaggggatca
     1501 gcgcgatgcc cagcgcgatc agcttggcgc gaaacacccg cagccggctg gggccctggg
     1561 tgaagagcgt cttgaaggtg tcccagccga agtcgctgcc gaggctcagc acgcccagca
     1621 tcagcgccag cgtgccgccg tagaagggga agccgccgat cagagagccc accaacccct
     1681 ggggcagcag cggcgccagc gacggcccgt tgccgaagtc ggcgcggcct ttgtaggtga
     1741 gatagggcaa gaggtacgcg aaggtgagcg ccagcgccag ccagatcccc agcagcaccc
     1801 agggagccac gcgcttgcgc agcactagta gttcggcggc gatactcccg ctcatgcgtc
     1861 ctctccttct ccttccaccg tctcccgcgt cagctcgagg aagacctgct ccagcgagtt
     1921 ctgctcgcga tagagctcat cgagtgcgac cccggcctcc accagcgagc gggcgatcac
     1981 ccgcgccgcg ccgggtcccg ctgcgatgcg cagcgtgtcg tccagcggcg tgacatcctc
     2041 gacctccggc agcgccagca cgacgcgccg cgcggtctcc aacggtgaag cgcgcaggcg
     2101 caggtggtcg cggcctc
//
LOCUS       DASUBI010000775         5685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_63681, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000775 DASUBI010000000
VERSION     DASUBI010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63681"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFU72_15200"
     CDS             complement(<1..674)
                     /locus_tag="VFU72_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5424891.1"
                     /translation="MTLRPDQAREWSLVLDELGLAVPEERIAAALQRERPLAAARRAR
                     TVPPSHAVTVEAGEERRCRFIMNVLREAGVAPDALPRAQEAIKGALDSARMYVAYPDA
                     LPALHALWERGLKLGVVTNAWPSLPRILLDLGFGEYLGFWVVSELIGVEKPGPAIFER
                     ALEIGGTPPERALQVGDDLRTDVAGARAAGMQAVLLDRSGGAEAAESAAALDVPVIRT
                     LAVLPDL"
     gene            complement(858..1262)
                     /locus_tag="VFU72_15205"
     CDS             complement(858..1262)
                     /locus_tag="VFU72_15205"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU5424892.1"
                     /translation="MGFDPAIIEKARQEREVDLTTFGRTSGKPSRKTLWINTEGGRLF
                     VRSGQGLGRDWPRNVLANGRALLHIAGHDLPVRAVHVTDPALARQVSAISSRKYGTTP
                     QQLPDGQLTPAEQATFELMPAEGVEGGDLLPP"
     gene            1901..2803
                     /gene="lysX"
                     /locus_tag="VFU72_15210"
     CDS             1901..2803
                     /gene="lysX"
                     /locus_tag="VFU72_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEU5424893.1"
                     /translation="MRPVQEPRIAIIHDRLRAEERLLFAEFERRGISVARIDDRRAIV
                     DLNGAPWPYDVVIGRGVSQQRTLHLLSVLDAWGIPTLNRPEVIAICNDKLRSSARFAG
                     AGLPQPELRVAFSPEAALAAIEELGYPAVLKPAVGSWGRLLGKVNSRAGAETLLAHKE
                     ALGGFHHGTYYIQEYVEKAGRDIRAFVVGDETICAIYRDAEHWITNTARGGRASNCPV
                     TPEIDALCRRAARAVGGGVLAVDLFEDPARGLLVNEVNATMEFRNSIDTTGVNIPARI
                     VDYALELGVAHAGVRAQEPVALVS"
     gene            complement(2951..>5685)
                     /locus_tag="VFU72_15215"
     CDS             complement(2951..>5685)
                     /locus_tag="VFU72_15215"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HEU5424894.1"
                     /translation="YATRVLTSDLLASPVDLTNEIIFSAGCHSGYNTVNGADVPGVTQ
                     EPDWAQAAASRGVTLIAGTGYQYGDTDFIKYSEELYLNFATQLRAGNGAVPLGQALVA
                     AKVQFLGQTMQRSGIDQKAFLEATLYGLPMLGVNMPGQRGTSTKPSIVSSTSPVSGSP
                     GAMLGLTSADVTLNPNLTKQTVTLRDIGGTGTHTATYYSSPDGVISMPFQPVLPLGRE
                     NVSVPGTVVRGVGFLGGSYTDNPNLLPLTGAATSDLRGVHAAFQSTTFYPAMPWSLNF
                     YDQLAGSGPPWLQVAEAQFKQSDPASLTGTMRVYNQMNFRLFYSNNIATYPAGGSYPT
                     TGDTPALAAAPAITRVSATLNNGVITFSANAVGDPTAGIQTVWVTYTLPPANGGNGQW
                     VSLPLSMTSFDSTLWQGTLPLSALGTTDASTLRFIVQAASGTGLVTLATNLGAYYSVG
                     VPASAGAPTQLSVTTSSNLTGAFGTSLTVSSLLTDQAGTPLPNQTVFFAIGAQRYAAT
                     TGSVAPNVGVATTVIQLLVPPGPAKLSVSFNGTDVYAPSSASTTVTVTQMATKLTFKP
                     NPVTVQYSDPNNLVATLTDIDGRPLGQRTIIFTIDGASAIDQSAIHQVVNAVTDFAGR
                     ASPGPLNTLPPGSYTVTGNFGNVVTVAGQTITMTDPRFLPVTASVPYTVTPEAATVTY
                     TGDTFVQAGSPITLSATVTQDPDGYPGDITLAQVQFTITSSSGTVVQTVLAPVSAAGT
                     ATTTVTLANPDVYTVQAQVVGGWFTSDPSSPVFVDNAPGAVNGTTLVTGGGWVNLAPG
                     AYKTNPAASGRANFGFNVTQAKGKISGNVQFNFSAGGLNFKGPNVTSLSVKQGTATLG
                     GSGTINGSGNYTFQLIVFDGGNTGDTFRLVIKSGTKTVFDSGTLPPVGGNIVIH"
BASE COUNT          874 a         1905 c         2035 g          871 t
ORIGIN      
        1 agatcgggaa gcaccgccag cgtgcggatc accggaacgt cgagcgcggc ggccgactca
       61 gccgcctcgg cgccgccgct ccggtcgagc agcaccgcct gcatgccggc cgcccgcgcg
      121 cccgccacgt cggtgcgcag gtcgtcgccg acctggaggg cgcgctcggg cggcgtgccg
      181 ccgatctcca gcgcccgctc gaagatggcc ggccccggct tctcgacgcc gatcagctcg
      241 gagacgaccc agaagccgag gtactcaccg aagccgagat cgagcaggat gcgcggcagg
      301 gaaggccagg cgttggtcac cacgcccagc ttgaggccgc gctcccagag cgcgtgcagc
      361 gccggcagcg cgtcggggta ggccacgtac atgcgcgccg agtcgagcgc ccccttgatc
      421 gcctcctgcg ccctcggcag cgcgtcaggc gcgacgccgg cctcccgcag cacgttcatg
      481 atgaagcgac accgccgctc ctcgccggcc tcgaccgtga ccgcatggga cggcggcacc
      541 gtccgcgccc gccgcgccgc cgccagcggc cgctcgcgct gcagcgccgc cgcgatgcgc
      601 tcctcgggca cagcgagccc gagctcgtcc agtaccagcg accactcgcg cgcctggtcg
      661 ggccgcagcg tcaaaagcgt gccgccggca tcgaaaggtg atcagctcaa cggcgaagca
      721 gggcgaggac atgggagctc ctttgtggtg atggcaggcg atagtggacg ggctgtcgcg
      781 tatcgtgtga gatcggcgcc tcatgacgcg cggctccgtc cgggttagaa ccaccgcgcg
      841 aacccggcgc gacgccatca aggcggtagc aaatcgcccc cctccacccc ctccgccggc
      901 atcaactcga acgtcgcctg ctcggcgggg gtcagctggc catcgggaag ctgctggggc
      961 gtcgtgccgt acttccggga cgagatggcc gagacctgcc gcgccagggc gggatcggtg
     1021 acgtggaccg cgcgcaccgg caggtcgtgc ccggcgatgt gcagcagcgc gcggccgttg
     1081 gccagcacgt tgcgcggcca gtcgcgcccg agcccctgac cggagcgcac gaagagacgc
     1141 cctccctcgg tgttgatcca gagcgtcttg cgcgacggct tgccgctggt gcgcccgaag
     1201 gtcgtcagat cgacctcgcg ctcctgcctg gccttctcga tgatcgccgg atcgaacccc
     1261 accgtccgct ccttcgccac gcacgcacaa caaccttccc cccaatcttg cgcgctccgg
     1321 cccctgggcg caacgatcag cgcgggggat tgacagagaa ggataaaaca ggcagactat
     1381 gcctcgacaa tcgagttgcc caatggcgac gatgaggcga gtagccggcc ggaacggcac
     1441 agagagccgc ggatgctgcg aagcggcccg gaaagaccgg tgaagaagac ctcggagcct
     1501 cgctccgaac ggcggcatct gctagcccag taggcggcga cggtctggct cccgttagcg
     1561 agccgcctgt ggcctccgcg cgctcctccg cgcggccggc acaggcaaag gtcgcgcacc
     1621 gcgagggcgc gacgaagtgg ggtggtacca cgagccggac accgctcgtc cccaacgggg
     1681 cgagcggttt tgcgtgccct ggacggtgtt ggcgacaggc tacacggcga ggactgaggg
     1741 cagggaccgt ggcacgactc gggcaaccag cagggcagaa ccgggaatcc atctccacca
     1801 gcgggcgcat ggataaacag agcgggccgc gcgcgtcgcg ccgtggcccc ggcagccggc
     1861 ccgtccaccg actttgccgt tgctcgagga gtgaacatcc atgcgtccgg ttcaggaacc
     1921 ccgtatcgcg atcatccacg accgcctccg cgccgaggag cggctccttt ttgccgagtt
     1981 cgagcggcgc ggcatctccg tcgcgcgcat cgacgatcgc cgtgccatcg tcgatctgaa
     2041 cggcgcgccc tggccctacg acgtcgtcat cggccgcggc gtcagccagc agcgcacgct
     2101 ccacctgctg agcgtgctcg acgcctgggg catccccacc ctcaaccgcc ccgaggtcat
     2161 tgccatctgc aacgacaagc tgcgctcctc ggcgcgcttc gccggcgcgg ggctgccaca
     2221 gccggagctg cgcgtcgcct tctcgcccga ggcggcgctg gcggcgatcg aggagctggg
     2281 ctacccggcc gtgctcaagc ccgccgtcgg ctcctggggc cgcctgctcg gcaaggtgaa
     2341 cagccgcgcc ggcgccgaga cgctcctcgc ccacaaggaa gccctgggcg gtttccacca
     2401 cggcacctac tacattcagg agtacgtcga gaaggcgggg cgcgacatcc gcgccttcgt
     2461 ggtcggcgac gagacgatct gcgccatcta ccgcgacgcg gagcactgga tcaccaacac
     2521 cgcgcgcggc ggccgcgcct ccaactgccc ggtcacgccg gagatcgacg cgctctgccg
     2581 ccgcgcggcg cgggccgtcg gtggcggcgt gctggccgtc gatctcttcg aggatcccgc
     2641 gcgcgggcta ctggtcaacg aggtcaatgc caccatggag ttccgcaaca gcatcgacac
     2701 caccggggtc aatattccgg cgcgcatcgt ggactacgcg ctggagctgg gggtggcgca
     2761 cgcgggagtg cgcgcgcaag agccggtggc gctggtgtca tgagggtggg gagcgcgacc
     2821 tgaaggcggc gtctaccacc agggggagtg ggcggccaca gggggccgcc cctacaggat
     2881 aggcgaatgt tctcgatgcg gtaagggtag acttccgtgt ctgccctcgc ctgtttgcct
     2941 caatctaccg ctaatgaatc acgatgttcc cgccgaccgg tggcagcgtg ccgctgtcaa
     3001 agaccgtctt cgtgccgctt ttgatcacca gccggaaggt atcgccggtg ttgccgccgt
     3061 cgaagacgat caactggaag gtgtagttcc cgctgccgtt gatcgtgccg ctgccgccca
     3121 gggtggcggt gccctgcttg accgagagcg aggtcacgtt cgggcccttg aaattcaagc
     3181 cgcccgcgga gaagttgaac tgcacgttcc cgctgatctt ccccttggcc tgggtcacgt
     3241 tgaagccgaa gttggcgcgg ccgctcgccg cagggttcgt cttgtaggcc cctggcgcca
     3301 ggttgaccca gccgccgccg gtcaccagcg ttgtgccgtt gaccgcgccc ggcgcgttat
     3361 caacgaacac cgggctgctt gggtcggagg tgaaccagcc gcccaccacc tgggcctgca
     3421 cggtgtagac gtctgggttg gccaacgtca ccgtcgtggt cgcagtgccg gcggcgctga
     3481 ccggcgccag caccgtctgc accaccgtgc cgctgctgct ggtgatggtg aactgcacct
     3541 gagccagggt gatatcgccg ggatagccgt cggggtcctg cgtcaccgtg gccgagagcg
     3601 tgatcgggct gccggcctgc acgaaggtat cgccggtgta ggtcaccgtc gccgcctccg
     3661 gcgtcacggt gtagggcacc gaggccgtca cgggcaggaa gcgcgggtcg gtcatggtga
     3721 tggtttggcc ggcgaccgtc acgacgttgc cgaagttgcc cgttacggtg taactgccgg
     3781 gcggcagagt gttgagcggc ccgggcgagg cgcgcccggc gaaatcggtg acggcgttca
     3841 ccacctgatg gatggccgac tggtcgatgg cggaggcgcc gtcgatggtg aagatgatgg
     3901 tccgctggcc gagcgggcgg ccgtcgatgt ctgtcagcgt ggcgaccagg ttgttcggat
     3961 cgctgtactg caccgtcacc gggttgggtt tgaacgtcag cttggtcgcc atctgggtga
     4021 cggtcacggt ggtagacgcc gatgagggcg cgtagacgtc ggtgccgttg aagctgacgc
     4081 tcaacttcgc cggccctggc ggcaccagca actggatgac ggtggtagcc accccgacgt
     4141 tgggggcgac gctcccggtc gtggcggcgt agcgctgcgc cccgatcgca aagaagaccg
     4201 tctggttggg gagcggcgtg ccggcctgat cggtcagcag cgacgagacc gtgagcgagg
     4261 tgccgaaggc gccagtcagg ttcgacgagg tcgtgaccga cagttgcgtg ggggcgcccg
     4321 cggaggccgg cacacccacc gagtagtagg cgcccaggtt ggtcgccagc gtcaccagcc
     4381 cggtcccgct ggccgcctgc acgatgaagc gcagggtgct ggcgtcggtg gtgccgagcg
     4441 ccgagagcgg cagcgtcccc tgccagagcg tgctgtcgaa gctcgtcatg ctgagcggga
     4501 gcgaaaccca ctggccattc ccgccgttcg cgggcgggag ggtataggtg acccagaccg
     4561 tctggatgcc cgccgtcggg tcgccgaccg cgttggccga gaaggtgatc acgccgttgt
     4621 tgagcgtggc cgagacgcgc gtgatcgcgg gcgcggccgc cagcgcgggc gtgtcacccg
     4681 tggtcgggta gctaccgccc gccggatagg tggcgatgtt gttgctgtag aagagccgga
     4741 agttcatctg gttgtagacg cgcatcgttc cggtgagcga cgcggggtcg ctctgcttga
     4801 actgcgcctc ggccacctgg agccaggggg ggcccgagcc ggccagctgg tcgtagaagt
     4861 tcagcgacca gggcatcgcc ggatagaagg tggtcgactg gaaggccgcg tggacgccgc
     4921 gcaggtcaga ggtcgccgcg ccggtcaacg gcaacagatt gggattgtcg gtgtagctgc
     4981 cgccgaggaa gccgacgccg cgcaccacgg tgccgggcac cgagacattc tcgcgcccga
     5041 gcggtaatac cggctggaac ggcatgctga tcacgccgtc cgggctgctg tagtaggtgg
     5101 cggtgtgggt gcctgtgccg ccgatgtccc gcagcgtgac ggtctgcttg gtcagattcg
     5161 ggttgagagt gacgtcggcg gaggtgaggc cgagcatggc gccggggctg ccgctgaccg
     5221 gtgaggtcga cgagacgatc gacggcttgg tgctcgtgcc gcgctggccc ggcatgttga
     5281 cgccgagcat gggcaggcca tagagcgtcg cctccaggaa ggccttctga tcgatcccgc
     5341 tgcgctgcat cgtctgcccc aggaactgca ccttggcggc gaccagcgcc tggcccagcg
     5401 gcaccgcgcc gttgccggcg cgcaactgcg tggcgaagtt gaggtagagc tcctcggagt
     5461 acttgatgaa atcggtgtcg ccgtactggt agccggtgcc ggcgatcagg gtgacgccgc
     5521 ggctggcggc ggcctgcgcc cagtccggct cctgcgtcac acccggcacg tcagccccgt
     5581 tgaccgtgtt gtagccggag tgacagccgg cgctgaagat gatctcgttg gtcaggtcga
     5641 ccggcgaggc cagcaggtcg ctggtcagga cacgcgtcgc gtagt
//
LOCUS       DASUBI010000776         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1042443, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000776 DASUBI010000000
VERSION     DASUBI010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042443"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFU72_15220"
     CDS             <1..939
                     /locus_tag="VFU72_15220"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424895.1"
                     /translation="GASYGSVLGAVYANLFPATTGHLVLDGSFDPVAWSRGTLPASLR
                     LGQDLATSAALRSYLELCGQAPASACAFSAGTPSATEAKFARLEQIVRRHPVMFGNPP
                     QPITLAKLLLALPLGDVDQWQGSTALLQQFWLAATSSSGSAGARSFWRPAHAAASGAP
                     YTGLEQTYAVSCADEADPRNVGAYSTAARLAQARAGGYGLYFTWTDEPCAHWPGTGAE
                     DKYSGPWNRPTASTILVLSLTGDPLTSNQDAIAMSRDLARARLLTVAGWGHTELANPS
                     NCAIRYEVSYLTTGALPPDGTVCAQNVAPFPVPSSG"
     gene            976..>2033
                     /locus_tag="VFU72_15225"
     CDS             976..>2033
                     /locus_tag="VFU72_15225"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5424896.1"
                     /translation="MRSEKGQHRPGFAYGRYVSAESDISYPLDGSAALPSYPPTAGRP
                     RPPLTKRLRPGHWMAIDLVVGGLVGLSAVLASTHGVLALPSRFLLAALIAVPVALRRR
                     YPLPAFCALIVVAVGATAAGFALPAGPSFIFATSAYALYTVAVCCARRVSVAALLAAF
                     AVGLATQLPVYQHHRSAGNLAWPAFALIIAWMTGHSARQRRRYAQMLQQEAASSAVAG
                     ERLRIARELHDVVAHSMSVIAVQAGYGQYVIDTSPADASEALGAIQATSRDALEEMRR
                     MLGVLRQQDPELHRPRSAPLAPAPDLDDLDRLIQRTRGAGIQVSLERSGPIRAVPAGV
                     GLSAYRIIQEALTNVVRHA"
BASE COUNT          275 a          770 c          681 g          307 t
ORIGIN      
        1 ggcgcgtcct acggcagcgt actgggcgcc gtctacgcga acctgttccc ggccacgacc
       61 gggcacctgg tcctggacgg cagcttcgac cccgtcgcct ggagccgagg gacgttgcct
      121 gcctcgcttc ggctaggaca agacctggcc acctcggcgg cgctgcggtc atatcttgaa
      181 ctgtgcggcc aggcgcccgc ttcggcgtgc gcgttctcgg ccggaacgcc gtccgctacc
      241 gaggccaagt tcgcccggct tgagcagatc gtgcgacggc atccggtgat gttcggcaac
      301 ccgccgcagc cgattaccct ggccaagctg cttctcgccc taccgctagg cgacgttgac
      361 cagtggcagg gcagcaccgc gctgctccag caattctggc tcgccgccac ctcgtcttcg
      421 gggtccgcgg gcgctaggtc tttctggcgc ccggcgcacg ccgccgcgtc cggcgctccc
      481 tacaccgggc tagagcagac gtacgccgtc agttgcgccg acgaggcgga cccgcgcaac
      541 gtcggcgcct actcgaccgc cgcacggctg gcgcaggcca gggccggcgg ctacgggctg
      601 tatttcacct ggaccgacga gccttgcgcg cactggcccg gcaccggagc ggaggacaag
      661 tactcgggcc cgtggaaccg ccccaccgcc agcaccatcc tggtgctgag ccttaccggc
      721 gacccgctga ccagcaacca ggacgcgata gcgatgtccc gtgacctcgc cagggcgcgg
      781 ttgctgaccg tcgcgggctg ggggcacacc gagctcgcca acccgagcaa ctgcgccatc
      841 cgctacgagg tcagctacct gaccaccggc gccctgccac cggacggcac cgtctgcgcc
      901 cagaacgtgg ctccgttccc cgtcccgtcc tccggctgaa ggcagggcga catcctaagc
      961 acccgagccc gggcggtgcg gtccgagaag ggccagcacc gccccgggtt cgcttacggt
     1021 agatacgtgt ccgccgaaag tgacatttcg tacccgctcg acggctcggc agcgctgccg
     1081 tcttatccgc cgacggccgg tcgccctcgc ccgccgctga ccaagcgcct gcggcccggg
     1141 cactggatgg cgatcgacct cgtggttggc gggctggtcg gcttgtccgc ggtgctcgcc
     1201 tcgacgcatg gcgtcctcgc gctgccgtcc aggttcctgc tcgccgcctt gatcgccgtc
     1261 ccggtcgcgc tgcgccgtcg ctatccgctg cccgcgttct gcgcgctgat cgtcgtggcg
     1321 gtcggcgcga ccgcggccgg ctttgccctc ccggccggac cgtcgttcat cttcgccacc
     1381 tcggcgtacg cgctttacac cgtggcggtc tgctgcgccc ggcgggtcag cgtcgccgcg
     1441 ctcctggccg cgttcgcggt gggcctggcg acgcaactcc ctgtctacca gcaccaccgc
     1501 agcgccggga atctggcgtg gccggcgttc gccctgatca tcgcctggat gaccggccac
     1561 tcggcgcggc agcgccgccg gtacgcgcag atgctgcagc aggaggcggc gagcagcgcg
     1621 gtcgccgggg agcggctgcg gatcgcgagg gagttgcacg atgtggtcgc gcacagcatg
     1681 agcgtgatcg ccgtccaggc gggctacggc cagtacgtga tcgacaccag cccagccgac
     1741 gcgagcgaag ccctcggcgc gatccaggcg accagccggg acgcgctgga ggagatgcgc
     1801 cggatgctgg gcgtgctcag gcagcaggac cccgagttgc accgcccccg ctccgcgccg
     1861 ctggcacccg ctccagacct cgacgacctc gatcggctca tccagcgcac tcgcggagcc
     1921 ggcatccagg tcagcctgga acgatccggc ccgatccgcg cggtgcccgc gggtgtcggc
     1981 ctgtcggcct accggatcat ccaggaagca ctcaccaacg tagtcaggca cgc
//
LOCUS       DASUBI010000777         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_997420, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000777 DASUBI010000000
VERSION     DASUBI010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997420"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="VFU72_15230"
     CDS             complement(<1..2394)
                     /locus_tag="VFU72_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5424897.1"
                     /translation="MKLSHFFIDRPIFAAVLSTFIFIAGAIALTQLPISEYPEVVPPS
                     IVVRATYPGANPKVVANTVAAPLEQAIVGVENMLYMSSTSTMDGSLALTITFRVGTDI
                     DRAQTLVQNRVSQVLPRLPDEVRNLGVTTTKSSPNLTMVVHIYSPDKRFDTLYLRNYA
                     VRNIRDVLARLPGMGDVQLFGGGDYAMRVWLDPQKLASLGMTAGDVVNAIRAQNIQVA
                     AGQIGAPPDPTTDFQLALNAMGRLTTPDQFRNIVLKTGADGQVVRLGDVARVNMGAQT
                     YGINSYLDNQGAVAIPVFQAPGSNALQLSQEVRTTMAQLSKDFPQGMAYTIVYDPTVF
                     VRESIDAVVHTLLEAVLLVVLVVILFLQTWRASIIPLIAVPVSIIGTMAVLLAFGFSI
                     NTLSLFGLVLAIGIVVDDAIVVVENVERHIESGQSPREAAKSAMSEVSRPIIAITLVL
                     CAVFVPVAFVSGLTGEFYRQFALTIAISTVISAFNSLTLSPALAAVLLKPHGTKPDAL
                     SRAMDRVLGGFFRRFNRGFTTLGNTYERAVVGTVRRIGLAVIVYAGLIGLAYLGFAAV
                     PHGFIPSQDKQYLVAIAQLPPASSLDRTSQVIKEIGDIGLHQPGIEHAVQFAGMSVNG
                     FVQSSSAGLVFFGLKPFDERRGKGLSADAIAAALNRKLSSIQDGMVVVFPAPPVMGLG
                     TLGGFKLQIEDRSDAGPKALYEATQAVLKKGYANPALAGLFSSYQVNVPQLNVNVDRV
                     KVMQDHVNLDDVFQTLQVYLGSIYVNDFNAFGRTYQVLAQADAPFRSRIEDISTLQVR
                     "
     gene            complement(2391..3641)
                     /locus_tag="VFU72_15235"
     CDS             complement(2391..3641)
                     /locus_tag="VFU72_15235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5424898.1"
                     /translation="MRNQSIAFLSLVPLAAAALAAVLTLSGCAGDASAQHAAMPPPAP
                     QVTVARVIARTVTDSETFSGRFSAVDHVDIRPRVSGYISSVNFVDGSVVHKGQVLFVI
                     DPRPYEAESQRAEADLDQARAQAGLAKSEQTRAVNLYAAHAISKDEFDTRAANAHQSA
                     ANVEAAKAAVDAAALNLTFTRVTAPITGRVSRAIVTTGNLVTNGQTLLTTIVSLDPIY
                     VEFNADEQAYLAFEKHASEGERKTARTSAGPDALGNAVYVGLADENGYPHQGRLVFMD
                     NSLNSQTGTIYARALLANPEGHFVPGLFARVKLIGNDRYQAVLINDSAIGTDQTMRYV
                     LLLGADNKVEYRPVELGPLVDGLRVVRSGLKPGDTIVVNGLMRVRPGMHVDPQLIAMG
                     ERHHNDGTMVAQNATGDSASRENR"
     gene            complement(3833..>5025)
                     /locus_tag="VFU72_15240"
     CDS             complement(3833..>5025)
                     /locus_tag="VFU72_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5424899.1"
                     /translation="AANDDSTGSSPARKGRGRGTARRATDSISSGRKVRYAVVGLGHI
                     AQVAILPAFAQAQQNSTLAALVSDDPEKLRKLSRRYGLRHVCSYDDADELFESGEVDA
                     VYITLPNSMHREYTERAARAGLHVLCEKPMAVTSEDCERMLEVTRENDVKLMIAYRLH
                     FERGNLEAAEMAHSGTLGDLRFFTSNFSMQIAPENFRSSRKLGGGPLYDIGIYCINAA
                     RAAFAAEPTEVLATAVTRRDHRFKEIPETVAVVLKFPQGRIASFTCSFGGADRSAYEI
                     VGTKGSITLDPAYEYAQGVEYVLRLGKRKREKRYEKSDQFAPELVYFSDCVLRDSEPE
                     PSGEEGIADVRVIEALNRSIETGRWVSPELPQRRRRPTLRQEIRRPGIQPPPLVGGVH
                     SASM"
BASE COUNT          791 a         1457 c         1934 g          843 t
ORIGIN      
        1 gcggacctgc agcgtggaga tgtcctcgat gcgggagcgg aacggcgcgt cggcctgcgc
       61 caggacctgg tacgtgcgcc cgaaggcgtt gaagtcgttg acgtagatgg agcccaggta
      121 gacctgcagc gtctggaaga cgtcgtccag gttgacgtgg tcctgcatga ccttgacgcg
      181 gtccacgttg acgttgagct gcggtacgtt gacctggtag ctgctgaaga ggccggcgag
      241 cgcggggttc gcatagccct tcttcaggac ggcctgtgtg gcttcgtaaa gcgccttggg
      301 gccggcgtcg ctgcgatcct cgatctgcag cttgaagccg cccagggtgc cgaggcccat
      361 gaccggcggc gccgggaaca cgaccaccat gccgtcctgg atggaggaga gcttgcggtt
      421 gagcgccgcg gcgatggcat ccgccgagag gcccttgccc ctgcgctcat cgaagggctt
      481 gaggccgaag aagacgagtc cggcgctcga gctctgcacg aagccgttga ccgacatgcc
      541 ggcgaactgc acggcgtgct cgatgcccgg ctgatgcagg ccgatgtcgc cgatctcctt
      601 gatgacctgc gaggtccggt cgagggagga ggcgggcggc agctgcgcga tggcgaccag
      661 gtactgcttg tcctgggagg ggatgaagcc gtgcggcacg gcagcgaagc ccaggtaggc
      721 gaggccgatc aggccggcgt acacgatgac ggcgaggccg atgcggcgga cggtgccgac
      781 gacggcgcgc tcgtaggtgt tgccgagcgt cgtgaacccc cggttgaagc ggcggaagaa
      841 gccgccgagc acccggtcca tggcgcgcga gagcgcatcg ggcttggtgc cgtggggctt
      901 gagcagcacg gcggccagcg cgggcgagag ggtgagggag ttgaaggcgg agatcaccgt
      961 ggagatggcg atcgtcaggg cgaactggcg atagaactcg ccggtgaggc cgctcacgaa
     1021 ggcgacgggc acgaacacgg cgcagaggac cagggtgatg gcgatgatgg ggcggctgac
     1081 ctcgctcatc gcggacttgg cggcctcacg cgggctctgt ccgctctcga tgtggcgctc
     1141 gacgttctcc acgaccacga tggcgtcgtc cacgacgatg ccgatggcga gtaccaggcc
     1201 gaagagcgac agggtgttga tggagaagcc gaaggcgagc agcaccgcca tggtgccgat
     1261 gatcgacacc ggcacggcga tgagcgggat gatggaggcg cgccaggtct gcaggaagag
     1321 gatcaccacc agcacgacca gcagcacggc ctcgagcagg gtgtgcacga cagcatcgat
     1381 cgactctcgc acgaataccg tcgggtcgta gacgatggtg taggccatgc cctgcgggaa
     1441 gtccttagag agctgcgcca tggtggtgcg cacttcctgg gagagctgca gcgcgttgga
     1501 gccgggggcc tggaagacgg ggatggcgac tgcgccctgg ttgtccaggt agctgttgat
     1561 gccgtaggtc tgggcgccca tgttcacgcg ggcgacgtcg cccaggcgca cgacctggcc
     1621 gtcggcgccg gtcttgagca cgatgttgcg gaactggtcg ggcgtggtca ggcgccccat
     1681 ggcgttcagg gcgagctgga agtcggtggt cgggtcgggc ggggcgccga tctgaccggc
     1741 ggccacctgg atgttctgcg cgcggatggc gttgaccacg tcaccggcgg tcatgccgag
     1801 ggaggcgagc ttctggggat cgagccagac gcgcatggcg taatcgccgc cgccgaacag
     1861 ctgcacgtcg cccatgccgg gcaggcgcgc gagcacgtcg cggatgttgc ggacggcgta
     1921 gttgcgcagg tagagggtgt cgaagcgctt gtccggcgag tagatgtgca ccaccatcgt
     1981 caggttgggc gagctcttgg tggtggtgac gccgagattg cggacttcgt cgggcagtcg
     2041 cggcagcacc tgcgagacgc ggttctgaac cagtgtctgg gcgcggtcga tgtccgtgcc
     2101 tacgcggaag gtgatggtca gcgcgagcga cccgtccatg gtcgacgtcg aggacatgta
     2161 gagcatgttc tcgacgccga cgatcgcctg ctccagcggc gcggccacgg tgttggccac
     2221 gaccttgggg ttggccccgg ggtaggtcgc gcgcacgacg atggagggcg ggaccacctc
     2281 cgggtactcg ctgatcggca gctgggtcag cgcgatcgca cccgcgatga agatgaaggt
     2341 ggagaggacc gccgcgaaga tcgggcggtc gatgaagaag tgcgaaagtt tcaccgattc
     2401 tccctggagg cggagtcgcc cgtggcgttc tgggccacca tcgtgccgtc attgtgatga
     2461 cgctcgccca tggcgatcag ctgcgggtcc acgtgcatgc cgggacgcac gcgcatcagg
     2521 ccattgacga cgatggtgtc gccgggcttg aggccggacc gcaccacgcg caggccatcg
     2581 accagagggc cgagctcgac ggggcggtac tccactttgt tgtcggcacc gaggagcagc
     2641 acgtagcgca tggtctggtc ggtgccgatg gcgctgtcgt tgatcagcac ggcctggtag
     2701 cggtcgttgc cgatcagctt gacgcgggcg aagaggccgg ggacgaagtg gccctcggga
     2761 ttggcgagca gcgcccgggc gtagatggtg ccggtctggg agttgaggga gttgtccatg
     2821 aagacgaggc ggccctggtg ggggtacccg ttctcgtccg cgaggccgac atacacggcg
     2881 ttgcccagcg cgtcggggcc ggcactggtg cgcgcagtct tgcgctcacc ctcactggcg
     2941 tgcttctcga acgcgaggta ggcctgctcg tcggcgttga actcgacgta gatcgggtcc
     3001 agcgacacga tggtggtgag cagggtctgg ccgttggtga ccaggttgcc ggtggtgacg
     3061 atcgcgcggc tgacgcggcc ggtgatgggg gccgttacgc gggtgaaggt gagattgagc
     3121 gcggcggcgt ccaccgctgc cttcgccgcc tcgacgtttg ccgcggactg gtgggcattg
     3181 gcggctcggg tgtcgaactc gtccttggag atggcgtggg cggcgtagag gttcaccgcg
     3241 cgggtctgct cggacttggc gaggccggcc tgggcgcggg cttggtcgag atcggcctcg
     3301 gcgcgctgag actcggcttc gtaggggcgg ggatcgatga cgaagagaac ctggcctttg
     3361 tggacgacgc tgccatcgac gaagttgacg gacgagatgt agccggacac ccgcgggcgg
     3421 atgtcgacgt ggtcgacggc gctgaagcgg ccgctgaagg tctccgagtc ggtgacggtc
     3481 cgtgcgatga cgcgcgctac ggtgacctgc ggcgccggag gcggcatggc ggcgtgctga
     3541 gcagacgcat cgcccgcgca gccggacagg gtcaggacgg ccgccagtgc ggcggcggcg
     3601 agcgggacga gcgagaggaa agcgatggat tgattgcgca cgaggggact ccctttgtat
     3661 tccctgagct agacgcagtc gagcctcacg gttcgacgac gtcgggcgac aattctggtg
     3721 ttgacaagcc cttggggcta tggctcgcgc ggaggggccg ccgcgtgtcc ggcggcgggg
     3781 ggaagtctct ctcttagtag gtagatgcgg gaggccgtgc ctcccgcaag cgctacatcg
     3841 acgcggaatg cactccgccg acgagcggcg ggggctggat gccgggacgg cggatttcct
     3901 ggcgcagtgt ggggcggcgc cggcgctgcg gcagctcggg tgagacccat ctgccggtct
     3961 cgatgcttcg gttcagcgcc tcgatgacgc ggacgtcggc aattccttct tctccggacg
     4021 gctccggctc ggagtcgcgc agcacgcaat cggagaagta aacgagctcc ggcgcgaact
     4081 gatcgctctt ctcgtagcgc ttctcgcgct tgcgcttgcc caggcgcagg acgtactcga
     4141 cgccctgggc atattcatag gcggggtcga gggtgatgct gcccttggtg ccgacgatct
     4201 cgtaggcgga gcggtcggct cctccgaagc tgcaggtgaa agatgcgatc cgcccctggg
     4261 ggaatttcag gacgacggcc accgtctccg ggatttcctt gaagcggtgg tcgcggcggg
     4321 taacggccgt ggcgagcacc tccgtcggct ccgcggcgaa tgcggcgcgg gcggcgttga
     4381 tgcagtagat gccgatgtcg tagagcgggc cgccaccgag cttgcggctg ctgcggaagt
     4441 tctcgggcgc gatctgcatg gagaaattgg aggtgaagaa gcgcaggtcg ccgagcgttc
     4501 cggagtgcgc catctcggcc gcttccaggt tgccgcgctc gaagtgcagg cggtaggcga
     4561 tcatgagctt gacgtcgttc tcgcgcgtca cctcgagcat ccgctcgcag tcctccgagg
     4621 tgactgccat gggcttctcg caaaggacgt gcaggccggc gcgggcggca cgctcggtgt
     4681 actcgcggtg catcgagttc ggcagcgtga tgtagacggc atcgacctcg ccgctctcga
     4741 agagctcatc ggcatcgtca tagctacaga cgtggcggag gccgtaacgg cgggaaagct
     4801 tgcggagctt ttccgggtcg tcggagacca gcgccgcgag cgtgctgttc tgctgcgcct
     4861 gggcgaaggc gggcaggatc gcgacctgtg cgatgtggcc gaggcctacg acggcgtagc
     4921 ggactttccg gcccgaggag atgctgtcgg tggcgcgccg agcggtgccc ctgccgcggc
     4981 ctttgcgcgc cggactggag ccggtgctgt cgtcgttggc ggcga
//
LOCUS       DASUBI010000778         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_369196, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000778 DASUBI010000000
VERSION     DASUBI010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369196"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFU72_15245"
     CDS             complement(<1..229)
                     /locus_tag="VFU72_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424900.1"
                     /translation="MVSPLRRSIAWLLRLLLVCASLVIALQADLIPKLLRSLAGWTTP
                     QWPPQATDATPLTPGLRLEDGLHALYTALTLG"
     gene            complement(240..322)
                     /locus_tag="VFU72_15250"
     tRNA            complement(240..322)
                     /locus_tag="VFU72_15250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Leu,seq:taa)
     gene            complement(410..841)
                     /locus_tag="VFU72_15255"
     CDS             complement(410..841)
                     /locus_tag="VFU72_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424901.1"
                     /translation="MSQQETAQALVQVLSEHRDDLITMLVEQAPAAGSGYEQTPPDAL
                     RPRMASVVDACIASLARQDPGLLRGFMRAAAEQRLREGYSVDSLITLALFTEGALSDT
                     AEIAFEAAPERRKEARKLIRALIAAAEEVLDPIAASTPDES"
     gene            complement(920..994)
                     /locus_tag="VFU72_15260"
     tRNA            complement(920..994)
                     /locus_tag="VFU72_15260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(960..962),aa:Gly,seq:gcc)
     gene            complement(1043..1117)
                     /locus_tag="VFU72_15265"
     tRNA            complement(1043..1117)
                     /locus_tag="VFU72_15265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Gly,seq:gcc)
     gene            complement(1175..2524)
                     /locus_tag="VFU72_15270"
     CDS             complement(1175..2524)
                     /locus_tag="VFU72_15270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5424902.1"
                     /translation="MNSTFGHLVIIAVLILINAFLNMAEIATVSVRRARIKQLADDGD
                     QQAQRVQRLLANPRTFIATVQIGVTLTALFTSAFGAVSLVEEASKGLVSAFGLAIQTA
                     NTVALVLITIAEAFLVLIFGELVPKTFGIEMAEPIALRVSGFVAILARVMWPAVAFLS
                     GTTNLVVRLLGGRRNAQIPSVTEEEIISMVETGEDEGVLTPSEQEMIRNIFEFTDRRV
                     SEVMVPRPDMFELSGRMTIAEAGPLVRKSGYSRIPIYEQDRENITGMVFAKDVLGAYI
                     EGRIDQPLQKIVHPVVFVPETKQIGELLQELQRSRQNLAIAVDEFGGVSGMLTLEDLL
                     EEIVGDIQDEFQHEEAPLQRVAPDELLVAGNLLLREVVDAFDLRVRDWDLDSTVAGLI
                     LELQGRIPDPGDSITWHGMTFTVAEMNGRRIQKVRVKLDEAPSVPAEEPGTTVAAGE"
BASE COUNT          469 a          866 c          860 g          471 t
ORIGIN      
        1 ggccgagggt gagggccgta tacagggcgt gcaggccgtc ttccagacgc aggcccggcg
       61 tcagcggggt ggcgtcggtg gcctgtggcg gccactgggg ggtggtccac ccggccagcg
      121 agcgcagcag cttggggatc aggtcggcct ggagcgcgat gaccagggat gcgcagacaa
      181 gtaaaagccg gagcagccat gcgatgctcc ggcgtagggg tgagaccata aaatcagggt
      241 gccagagagg ggagtcgaac ccctacggcc ttgcggccag cggtgtttaa gaccgcagcg
      301 tctgccattc cgccactccg gctcgtcctt ccgtgagtaa ttgtcgccta cgcgctgggc
      361 tggccggttt gaaaccggcc ctacatcgca ctatcaggcg ggccggctac tagctctcgt
      421 cgggggtgct cgcagcgatc gggtcgagca cttcctcggc agcagcgatc agcgcacgga
      481 tgagcttgcg cgcttccttg cgccgctccg gcgcggcctc gaaggcgatc tccgcggtgt
      541 cgctgagcgc gccctcggtg aagagcgcca gcgtgatcag cgaatcgacc gagtagccct
      601 cgcgcaggcg ttgctcggcg gctgcgcgca taaaaccgcg cagcaagccg gggtcctggc
      661 gcgcgaggct ggcgatgcag gcatcgacca cgctcgccat gcgaggccgc aatgcgtcgg
      721 gcggggtctg ctcatagccc gaccccgcgg cgggcgcctg ttccaccagc atcgtaatca
      781 agtcatcgcg gtgctcgctc agaacctgaa ccagtgcctg tgccgtttct tgctgcgaca
      841 tgcctctacc ccgtgtgtgc tatgcttgcg gcccaaacaa aaaggggaag cgctcggctt
      901 tcccctcttt gtgctcgaat ggagcggaag accagattcg aactggcgac cctctccttg
      961 gcaaggagat gctctaccac tgagccactt ccgcgttcgg cgcccatccg ctactccggg
     1021 cgggccgtat atatcgttgg cgtggagcgg aagaccagat tcgaactggc gaccctctcc
     1081 ttggcaagga gatgctctac cactgagcca cttccgcgtt tgcgaccgta ttataccaaa
     1141 cggcacgcct gctgtcaaga gaacgccggc gggtttactc gcctgcagcc acggtggtgc
     1201 ccggttcctc ggcgggcacg gacggcgctt catccagctt gacgcgcact ttctggatgc
     1261 gccgcccgtt catctcggcc acggtgaagg tcataccgtg ccaggtgata gagtcgccgg
     1321 gatcggggat gcggccctgc aactccagga tcagcccggc caccgtgctg tcgagatccc
     1381 aatcgcgcac gcgcaggtcg aacgcgtcga cgacctcgcg cagcaggagg ttgccggcca
     1441 ccagtaactc gtcgggcgcg acgcgctgca agggcgcttc ctcgtgctgg aactcgtcct
     1501 ggatatcacc gacgatctct tcgagcaggt cttccagcgt cagcatgccc gacacgccgc
     1561 cgaactcgtc cacggcaatg gccaggttct ggcggctgcg ctggagctcc tgcaacaact
     1621 cgccgatctg cttggtttcg ggcacgaaga caaccggatg gacgattttc tgcaacggct
     1681 ggtcgatgcg cccttcgata taggcgccca gcacgtcctt ggcgaagacc atgccggtga
     1741 tgttctcgcg gtcctgctcg tagatcggga tgcgcgagta gcccgacttg cgcaccagcg
     1801 gccccgcttc ggcgatggtc atgcggcccg atagctcgaa catgtcgggg cgcggcacca
     1861 tgacctcact gacccgccgg tcggtgaact caaagatgtt gcggatcatc tcctgctcgc
     1921 tcggcgtcag caccccctcg tcttcgccgg tctcgaccat cgagatgatc tcttcctcgg
     1981 tcacgctcgg tatctgcgcg ttgcggcgcc cgcccagcag ccggacgacc aggttcgtgg
     2041 tgccggagag gaaggcgacg gcgggccaca tgacgcgcgc caggatcgcc acaaacccgg
     2101 agacgcgcaa ggcaatcggc tcggccatct cgatgccgaa ggttttgggc accagttcgc
     2161 cgaaaatgag caccagaaac gcctcggcga tggtgatgag caccagggca acggtgttcg
     2221 cggtctggat cgccaggccg aaggcgctca ccagcccctt ggacgcttcc tcgaccaggc
     2281 tgaccgcgcc aaaggccgag gtgaagaggg cggtcagcgt gacgccgatc tggaccgtgg
     2341 cgatgaaggt acgcggattc gccagcaggc gctgcacgcg ctgggcctgc tggtcgccgt
     2401 cgtcggccag ttgcttgatg cgcgcccgcc gcaccgagac cgtggcgatc tcggccatgt
     2461 tcaggaacgc gttaatgaga atgagcaccg cgataatgac gaggtgcccg aacgtgctat
     2521 tcacccaaaa ctccttatac catccgggat acgcaatcgg tatcccacgg gtcgcacaac
     2581 gtggcgcccg atcctattat tttaccaaat ttcgggccac agcggatctt gacaggccgc
     2641 gcggcggcgg cctataatcc gggcgc
//
LOCUS       DASUBI010000779         6830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_168432, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000779 DASUBI010000000
VERSION     DASUBI010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168432"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFU72_15275"
     CDS             <1..646
                     /locus_tag="VFU72_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5424903.1"
                     /translation="KTYDTGKVRVPALRGVDLTVHRGEMIAIMGPSGCGKTTLLNCLS
                     GLDTVDTGTIEIAGTDLAKLPDNARTEFRARNMGFIFQFYNLLPVLSAVENVELPLLV
                     NGVRPAEARRRALAALAQVGIAEWAEHKPAELSGGQRQRATIARALVNGPAIVWGDEP
                     TGDLDSETSNEVMELMRRLNRDNGQTFVLVTHDPRIGARCDRIVQMRDGRIIDG"
     gene            671..3658
                     /locus_tag="VFU72_15280"
     CDS             671..3658
                     /locus_tag="VFU72_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5424904.1"
                     /translation="MNAIFGIPTTSLMLALVVIFALCLSSAGFLLVRNRVVFRLGVRN
                     VPRRHAQTLLIVVGLMLSTLIIAAAFGTGDTLDHSIKSQVYEVYGQVDEQITLGGTDT
                     AAGLQTGAHMPESIVPGLDAATQNSQVIDGLMPAFAETVPAINNRTRLFEPGLIATGL
                     DPSRTPAFGGVMSVAGQPIDLSTLPAGAVVLNQRAADKLDAQPGDTLTIYVQNQPQQL
                     SVSAIAANSLLTGYTGAGSSGGFTMALSQAQSLFGTPGQLSQILVSNTGGVVSGVQQS
                     ERATSALNGALDGTPYRATASKQQQLDEAEQTGNSFLSIFLIFGLFSISVGILLIFLI
                     FVLLAAERQPEMGVARAVGMKRRQLTEMFLAEGIAYDLISALVGAALGVLVAFTMADF
                     MGRIFGDAFPIRATITWRSLVIAYTLGVVVTFASVLISAWRVSKLNIVAAIRDTPAAK
                     PRRESRRWLLFGALGLALGGLLVLVGASVGSQFPFLAGLSLIPLSLAAVLRRFGVSPR
                     PLYTIAAALVLILWLLPSSLTGRIYPHLTGGIEMFFLSGMMMVASATVLIVWNATIVT
                     RAVSLLGRASSRWLPAIKTAVAYPLERKGRTGMTIAMFSLVVFALVMMAAINANALQL
                     ITGSEYASGGWDIVAAAPPTNPISDFRAALSQNNVDPGQIAAIGRLSSMGVAGSRVQQ
                     EGAASSGQYPIYGADPSFLEQSRLPLQTRADGYADDAAVWQAVLREPDLALVDSNAIP
                     NPTTIRIGGQSFSLSGIEPNDKHMRPVPVQITNLASGQTRVVRVIGVLDTRVATFPGI
                     MLSEQTFQSIFPNPVSVEYQLRVAPGVAAAPLAKTIESSMVSYGVQAESYRALLDEAT
                     RNSRGFLTLIEGFMLLGLIVGIAALGVVSFRSVVERRQQIGMLRAIGYTRAMVAASFV
                     IESTMITVLGIVSGAALGLILAWRLFAGNYFFGTSSSVTFSIPGLEIAAFLAIALLAA
                     VLTAYLPARRAAGVTIAETLRYQ"
     gene            complement(3667..4395)
                     /locus_tag="VFU72_15285"
     CDS             complement(3667..4395)
                     /locus_tag="VFU72_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU5424905.1"
                     /translation="MHLTICWLYGAAMNIYGDRGNVIALAERCRWRGIDAEVVEVGVG
                     EPLEAGRYDIFFWGGGQDREQIAASADIQGAKGETLAREVEDGAPLLAVCGGYQLLGR
                     YYRPHDAPELPGIGIFDAWTEAGPERFIGNVVVESPELGQLVGFENHSGLTWLGTGAE
                     PLGRVVVGKGNNGQDGLEGCRYRHAIGCYLHGALLPKNPQVTDYLIRAALQRRYGVVE
                     LAPLDDSLEQTAHSTAMERAAATR"
     gene            complement(4549..5964)
                     /locus_tag="VFU72_15290"
     CDS             complement(4549..5964)
                     /locus_tag="VFU72_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEU5424906.1"
                     /translation="MSRAARPAGLDLRLATAVVASKLSGAAIRRLGRGGGTSAPGVVA
                     NLIDPNVLDKLSRRVEGGCVVVAGTNGKTTTARMLADILEAAGLRVIHNRSGSNLVRG
                     ISAAFTGQSTLRGRPRGRVAVVEADEAAFPEVVRRTRPRLVLLLNLFRDQLDRYGELD
                     TIARRWREALLALAPEQTVLINADDPALAALSEGIEARRLTFGLADSRYLLAALPHAA
                     DAALCRRCGAPLRYERLFLSHLGDYACERCGFHRPALDYAARDIALHALDSVALQVET
                     PDGDLPLTVAVPGLYNGYNALAAAAAARTLGVEPATVQAALARFQSAFGRIERVSYQG
                     RTLVIVLIKNPVGFNEVLRMLALSGLADPALIVINDLDADGRDVSWLWDADVEQLATV
                     PAPLYTSGIRGGDMAVRLKYAGVPQERIHPLGPLRAALGQFVEAVPVGGTAYVLPTYT
                     ALLEVRGILTERGAVGAFWEE"
     gene            complement(5961..6782)
                     /gene="mreC"
                     /locus_tag="VFU72_15295"
     CDS             complement(5961..6782)
                     /gene="mreC"
                     /locus_tag="VFU72_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5424907.1"
                     /translation="MLSTTARHTAVLVFCFVAFAGTLIVLDNHHRLDAAKTPLETLLH
                     PFVAGLSAAGDDLRGLSHRNQSDLAKQLQAVTAERDQLLAENAKLQQLQGEVDQLRKQ
                     LDFKEAHPGLTPLSANVIGHDPSAAHQILVLDRGSDDGIQKGMAVISPDFYVGQVSEV
                     TPERARVILAIDASAHVGGMVQRSKAIGVLDGRWQAGGYFQLDHLKASDDVVAGDVIV
                     TSAGTARVPEGLVVGKVSRVDREVQADTVTAQVTPLIDYSALRSVTVILSAGNGQ"
BASE COUNT         1032 a         2442 c         2269 g         1087 t
ORIGIN      
        1 gaagacctac gacaccggca aggtgcgggt gccggcgctg cgcggcgtcg atctgacggt
       61 gcaccgtggc gagatgatcg cgatcatggg tccctccggc tgcggcaaga ccaccctgct
      121 caactgcctc tccggactcg ataccgtcga caccggcacc atcgagatcg ccggaacgga
      181 tctggccaaa ctgcccgaca atgcccgcac cgagttccgc gcgcgcaaca tggggttcat
      241 cttccagttc tacaacctgc tgccggtgct ctcggcggtc gagaacgtcg agttgccgct
      301 gctggtcaac ggagtccggc cggccgaggc acgccggcgc gcgctcgcgg ccctggcgca
      361 ggtgggtatc gccgagtggg ccgagcacaa gccggccgag ctctccggcg gccagcggca
      421 gcgcgcgacc atcgcccgtg ccctcgtcaa tggccctgcc atcgtctggg gcgacgaacc
      481 caccggcgac ctcgacagcg agacatcgaa cgaggtcatg gagctcatgc gccggttgaa
      541 ccgcgacaac gggcagacat tcgtgctcgt cacgcacgat ccccgcatcg gagccaggtg
      601 cgatcgcatc gtccagatgc gggatgggcg catcatcgac ggctagccgc cggggttgag
      661 gagactcacc gtgaacgcga tcttcggcat cccgaccacg tcgttgatgc tggcgctcgt
      721 cgtcatcttc gcgctgtgcc tgagcagcgc cggcttcttg ctggtccgca accgcgtcgt
      781 cttcaggctc ggcgtgcgca acgtgccgcg acggcacgcc cagacactcc tgatcgtcgt
      841 cggactgatg ctcagcacgc tgatcatcgc cgccgcgttc ggcaccggcg atacgctcga
      901 ccacagcatc aagtcccagg tctacgaggt ctacggtcag gtcgacgagc agatcacgct
      961 cggtggcacc gacaccgccg cgggtctcca gaccggcgcg cacatgccgg agtcgatcgt
     1021 gcccggtctt gacgcggcca cccagaactc ccaggttatc gatggtctca tgccggcgtt
     1081 cgccgagacg gtgccggcca tcaacaaccg caccagatta ttcgagcccg gactgatcgc
     1141 gacgggcctc gatcccagcc ggacgcccgc cttcggtggc gtgatgagcg tcgccggcca
     1201 gccgatcgac ttgagcacgc tgccggcggg cgccgtggtg ctgaaccagc gcgcggcgga
     1261 caagctcgac gcgcaaccgg gagacacgct caccatctac gtccagaacc agccccaaca
     1321 gctcagcgtg tcggccatcg cggccaactc gctgctgacc ggctacaccg gcgccggctc
     1381 ctccggtggc tttaccatgg ctctctcgca ggcgcagtcg ctgttcggca cgccaggtca
     1441 gctcagccaa atcctggtca gtaacaccgg cggggtggtt tccggggttc agcagagcga
     1501 gcgcgcgacc agcgcgctga acggcgcgct ggatggtact ccgtaccggg ccaccgcgag
     1561 caagcagcag cagctcgacg aagccgagca aaccggcaac agcttcctga gcatcttcct
     1621 catctttggg ctcttctcga tctcggtcgg tatcctgttg atcttcctga tcttcgtgct
     1681 cctggcagcc gagcgccaac cggagatggg agtggcgcgc gcagtcggca tgaagcggcg
     1741 gcaactcacc gagatgttcc tggcagaagg catcgcctac gacctgatct cggcgctggt
     1801 cggtgcggcg ctgggggtgc tggtcgcctt caccatggcc gatttcatgg ggcggatctt
     1861 cggcgatgcg tttccgatcc gggccaccat cacctggcgc agtctggtga tcgcctatac
     1921 cctgggcgtt gtggtgacct tcgccagcgt gctcatctcg gcctggcgtg tcagcaagct
     1981 gaatatcgtc gccgcgatcc gtgatacgcc cgcggccaaa ccccggcgcg agagccggcg
     2041 ctggctgctc ttcggcgcgc tggggctggc gctcggcggc ctgctggttc tggtgggcgc
     2101 gtccgtgggc agccagttcc ccttcctggc cgggctctcc ctcatcccgc tcagcctggc
     2161 ggcggtgctg cggcgctttg gggtatcgcc gcggccgctc tacacgatcg ccgccgcgct
     2221 cgtgctcatc ctctggcttc tgccgtcgtc gctgaccggc cgcatctatc cgcatttgac
     2281 cggcggcatc gagatgttct tcctgtcggg catgatgatg gtcgcgtcgg cgaccgtgtt
     2341 gatcgtctgg aacgccacga tcgtcacgcg cgccgtgagc ttgctcgggc gcgcgtcgag
     2401 ccgctggctg ccggccatca agacggctgt cgcctatccg ctggagcgga aggggcgcac
     2461 cggcatgacc atcgcgatgt tcagcctggt cgtcttcgcc ctggtgatga tggccgcgat
     2521 caatgccaac gccttacagc tcatcaccgg cagcgaatac gcctcgggcg gttgggacat
     2581 cgtcgccgcg gcgccaccca ccaacccaat cagcgacttc cgggcggctc tgtcccaaaa
     2641 caacgtcgac cccggccaga tcgcggcgat cgggcgactg tcctccatgg gagtcgccgg
     2701 gtcgcgcgtg cagcaggagg gcgccgcgag ctccggccag tacccgatct acggagccga
     2761 cccgagcttc ctggagcaga gccggctgcc gctgcagacc cgggccgacg gctacgccga
     2821 cgacgccgcg gtctggcaag cggtcttgcg tgagccggac ctggcactgg tcgacagcaa
     2881 cgcgatcccc aatccgacca cgatccggat cggcggacag agcttcagcc tgtcggggat
     2941 cgagccgaac gataagcaca tgcggccggt gccggtccag atcaccaacc tggccagcgg
     3001 ccagacgcgc gtggtgcgcg tgatcggggt cctcgacacg cgtgtcgcga ccttccccgg
     3061 catcatgctc tcagagcaga ccttccagag catcttcccg aacccggtca gcgtcgagta
     3121 ccagctccgg gtcgcgccgg gagtcgccgc cgccccgctg gcaaaaacga tcgagtcgtc
     3181 catggtcagc tacggcgtgc aggcggagtc gtatcgcgca ctgctcgacg aggcgacgcg
     3241 caacagccgg ggcttcctca cgctgatcga agggttcatg ctgctcggac tgatcgtcgg
     3301 catcgcggcg ctcggcgtgg tctcgttccg ctcggtcgtc gaacggcgtc aacagatcgg
     3361 catgctgcgc gcgatcggct acacccgcgc catggtcgcc gccagcttcg ttatcgaatc
     3421 gaccatgatc acggtgctcg gcatcgtctc cggggcggcg ctggggctga tccttgcctg
     3481 gcggctcttc gccgggaact atttcttcgg cacctcgagc agcgtcacgt tcagtatccc
     3541 cgggctggag atcgccgcct tcctggccat cgcgctgctc gcggcggtcc tcaccgccta
     3601 tctgccggcc cggcgcgccg cgggcgtcac catcgccgag acgctgcgct accagtagcg
     3661 cgccggtcag cgtgtagccg ccgcccgctc catggccgtg gagtgggcgg tctgctccag
     3721 ggaatcatcc agcggggcga gttccacgac gccatagcgc cgctggaggg ccgcacggat
     3781 caggtagtcg gtcacctggg ggttcttggg gagcaaggcg ccgtgcaggt agcagccgat
     3841 ggcgtggcga tagcggcagc cctccagccc gtcctggccg ttgttgcctt ttccaaccac
     3901 cacgcgcccc agcggctcgg cgccggtgcc gagccaggtc agaccgctgt ggttctcgaa
     3961 tcccaccagt tgccccagct cgggtgactc gaccaccacg ttgccgatga agcgctcggg
     4021 ccctgcctcg gtccaggcgt cgaagatgcc gattccgggc agctccggcg catcgtgggg
     4081 ccggtagtac ctaccgagca attggtagcc gccgcagacc gccagcagcg gcgcgccgtc
     4141 ctcgacctcg cgcgccagcg tctcgccttt ggcgccctgg atgtcggccg aggccgcgat
     4201 ctgctcgcga tcctgcccgc cgccccagaa gaagatgtcg tagcgcccgg cctccagcgg
     4261 ctcgcccacg ccaacctcga ccacctcggc gtcgatgccg cgccagcggc atcgctcggc
     4321 cagcgcgatc acgttgcccc ggtcgccgta gatgttcatc gccgccccgt agagccagca
     4381 gatcgtcagg tgcatagtcg ggccctcacc cccggcccct ctcccaatgc tgggcgaggg
     4441 gagacgaggg aacgagggca gacacggagg tctgccccta caaccgtatc ttctccccct
     4501 tctcccagaa ttgggagaag ggggcggggg gatgagggcc gccggccctc actcctccca
     4561 gaaggcgccc accgcgccgc gctcggtcag gatgccccgc acctccagca gcgccgtgta
     4621 ggtcggcaag acgtaggccg tgccgcccac cggcacggcc tcgacgaact ggcccagcgc
     4681 cgcgcgcagc gggccgagcg gatggatgcg ctcttgaggc accccggcgt acttgagccg
     4741 caccgccatg tcgccgccac ggatgccgga ggtgtagagc ggcgccggaa ccgtcgccaa
     4801 ctgctcgacg tcggcgtccc agagccagga gacgtcgcgc ccgtcggcgt ccaggtcgtt
     4861 gatcacgatc agcgccgggt cggcgagccc gctcagggcc agcatgcgca gcacctcgtt
     4921 gaagccgacc ggattcttga tcagcacgat gaccagcgtg cgcccctgat agctcacgcg
     4981 ctcgatgcgc ccaaaggccg actggaagcg cgccagggct gcctgcaccg tcgccggctc
     5041 gacccccagc gtgcgggcgg ccgcggcggc ggccagcgca ttgtagccgt tgtagaggcc
     5101 cggcacggcc accgtcagcg gcaggtcacc gtccggtgtc tccacctgga gggcgacaga
     5161 gtcgagcgcg tgcagcgcga tgtcgcgcgc ggcgtagtcg agcgccggac ggtgaaaacc
     5221 acaccgctcg caggcgtaat cccccaggtg cgagaggaaa agacgctcgt agcgcagtgg
     5281 cgcgccgcag cggcggcaga gcgccgcgtc ggccgcgtgc ggcagcgccg ccagcaggta
     5341 gcgcgaatcc gccagcccga acgtcaggcg ccgcgcctcg attccctcgc tcagcgccgc
     5401 cagcgccgga tcgtctgcgt tgatcagcac cgtctgctcg ggcgcgagcg cgagcagcgc
     5461 ctcgcgccag cggcgggcga tcgtgtccag ttcgccgtag cgatcaagct ggtcgcgaaa
     5521 gagattgagc aggagcacca ggcgcggtcg ggtgcgccgg acgacttcgg gaaaggccgc
     5581 ctcgtcggcc tccaccaccg ccacccggcc ccgcggccgc ccgcgcagcg tgctctggcc
     5641 ggtgaaggcc gcgctgatgc cgcgcaccag gttcgagccg gagcggttgt ggatcacccg
     5701 caggccggcg gcctccagga tatccgccag catgcgcgcg gtcgtcgtct tgccgttggt
     5761 tccggccacc accacgcagc cgccctcgac tcgccggctc agcttgtcga gcacgttggg
     5821 gtcgatcagg ttggcgacca cacctggggc gctggtcccg ccgccgcggc ccagacggcg
     5881 aatggcggcc ccgctcagct tgctggcgac aaccgccgtc gccagccgca ggtcgagccc
     5941 cgcgggccgc gccgcccggc tcactggccg ttcccggcgc tgaggatgac cgtcaccgag
     6001 cgcagcgcgc tgtagtcgat gagcggtgtc acctgtgccg tgacggtatc ggcctgcacc
     6061 tcgcgatcga cgcgcgacac cttgccgacc accagcccct cgggcacccg cgccgtgccc
     6121 gccgaggtga cgatcacgtc cccggcgacc acgtcgtcgc tcgccttgag gtgatcgagc
     6181 tggaagtagc cgcccgcctg ccagcggccg tcgagcaccc cgatcgcctt gctgcgctgc
     6241 accatccccc ccacgtgcgc gctagcgtcg atcgccagga tcactctggc ccgctccggt
     6301 gtgacctcgc tcacctgacc gacgtagaag tcggggctga tgaccgccat ccccttctga
     6361 atgccgtcgt ccgaaccgcg gtcgagcacc aggatctgat gcgccgcgct cggatcatgg
     6421 ccgatcacgt tggcgctgag cggggtcagc cctgggtgcg cctccttgaa gtcgagctgc
     6481 ttgcgtagct gatcgacctc gccctgcaac tgctgcagtt tggcgttctc ggccagcagt
     6541 tggtcgcgct cggcggtgac cgcctggagc tgcttggcca ggtcggactg gttgcgatgg
     6601 gagagcccgc gcaggtcgtc accggcggcg ctcaggcccg cgacgaaggg gtggagcagc
     6661 gtctccagcg gcgttttggc ggcgtcgagg cggtggtgat tgtcgagcac gatcaacgtg
     6721 ccggcgaagg cgacgaagca gaagacgagc acggcggtgt gtcgcgcggt ggtcgagagc
     6781 atgattcccc gccggctacc tcaccggacg ccgcagctcc tggccgccgg
//
LOCUS       DASUBI010000780         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_961594, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000780 DASUBI010000000
VERSION     DASUBI010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961594"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1384
                     /locus_tag="VFU72_15300"
     CDS             170..1384
                     /locus_tag="VFU72_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU5424908.1"
                     /translation="MRAVVLSIGAELLEGFITDTNATFLAQELAALGVELVGVRQVGD
                     RLVDIVRELERGWQDAELIITTGGIGPTDDDLTREAIADLLGETPAVDEATLAIISRF
                     FGSRGITMPEQNTKQAWLIPSAEALPNPMGTAPGWFVQRQGRFIVTMPGVPREMRRMW
                     AEQAVPRLAPSLGDQVYVSRTLKTIGIGESAAEKEIAEIIHRGGPIVATYAKDDGVHV
                     RIVAGAPESGPAQQAVARTVAEVRAILGPYIYGELGVSLGSAILEPLLRTGATLALSE
                     VGSGGRLSELLVEEPTAERHLLASVVQPWEQAAAPGESVAEVAAREAVKARDRFGATF
                     GYAIAVRMEPGETPDVTRGHVALCLAGPGEPVERAHRVNAIPAEVRRRAGLWAAEFLW
                     KTLHEQVGAASG"
     gene            complement(1322..2377)
                     /locus_tag="VFU72_15305"
     CDS             complement(1322..2377)
                     /locus_tag="VFU72_15305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424909.1"
                     /translation="MTGFERPRVSLIVTVKDEAATVDALLASIAAQRLSPDEIIVVDG
                     GSHDGTVARLKDWQGRLPLTVLAAPGANIAAGRNVALARARGAIVAVTDAGVRLEPDW
                     LAELVAPFDRPPAAQPDVVSGFFRADPCSRFERVLGAVTLPDVNEIAPARFLPSSRSV
                     ALRRELIAAGFRYPEWLDYCEDLVFDLRLRAAGARFEFRPQAIARFRPRATLGAYTRQ
                     YFRYARGDGKAGLFARRHALRYVTYLGLLPLLFVARDRRLTAAALAGGAVYMRRPWLR
                     LWRQRLPLSQMLPALALAPALRLAGDLAKLVGYPAGLIWRARRCGLRRGRRAIPELAG
                     GCPDLLVQGLPEELGRP"
     gene            complement(2364..2810)
                     /gene="ybeY"
                     /locus_tag="VFU72_15310"
     CDS             complement(2364..2810)
                     /gene="ybeY"
                     /locus_tag="VFU72_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5424910.1"
                     /translation="MSAGPAFSVEIRLNPGAPSLNSDRVAALLDFAARQEAAAGEVGL
                     WVCDDSEIAALHQQFMGLSGPTDVMSFPGEGDYLGDIAVSFETAAQQALGAGHGTQRE
                     IAYLALHGFLHLLGFDDLTPEERARMLARQDALLADFERESPGDWV"
     gene            complement(2807..>4301)
                     /locus_tag="VFU72_15315"
     CDS             complement(2807..>4301)
                     /locus_tag="VFU72_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5424911.1"
                     /translation="DQTKAARDAAAKNVQSVMVTVQQGQVIVRDGDPVTSYDIEKLEH
                     FGLLTPSHNWPQFVGTLGLLALVTLALLGYLFKTASGTDDLRQLLLISLIVVVPVVTG
                     RFLLQSDQLRLMFPAAAAAMLLSILLDFQLAVVLAGFLALYLGIVSGSTFEVALLTFV
                     SSLAGAAIVYRAERIVRFFWAALGVALAVFATAICLALISGHVDAAEVGQLVGDAAVN
                     GAVSASLTFLFFYMLGRVFGIVTHLQLLELAHPNQPLLYRLAREAPGTYHHSIVVSNL
                     AESAVEAVGGDPLFARVACLYHDIGKVLRPSFFVENQANRANVHDALDPRTSARIIQE
                     HVEDGVRLGRKARLPQPIIDVIQQHHGTTLIKYFYAEALASGEPVDEAEFRYKGPKPQ
                     TKEAAIIMLSDSVEAAVRAASQSGKLYEEANGDGRRASTGLKEVVDGVIKARLEDRQL
                     DDCDLTLRQIEQIRATFITMLEGIYHPRVEYPELAKTAARQPVEAAH"
BASE COUNT          677 a         1491 c         1555 g          578 t
ORIGIN      
        1 gcagagactt ccatcgtccg cgcgatcgcg ctgtgtcccg gcggctccat aggcatacgc
       61 agatgcgccc gcgcttcgcg agcggcgttc cggagagctg cggcgcgaca ccagccggac
      121 gatgctatca tgccgcaggc gagtcggcag gagcggaaag ggtggcggca tgcgcgcggt
      181 ggtgctctcg atcggggcgg aactcctcga aggcttcatc accgatacca atgccacgtt
      241 tctggcgcag gagctggccg cgctcggcgt cgaattggtc ggcgtgcgcc aggtcggcga
      301 ccggctggtc gacatcgtgc gcgagctgga gcgcggctgg caggacgccg agctgatcat
      361 caccaccggc ggcatcggcc ccaccgacga cgacctgacg cgcgaggcga tcgccgacct
      421 gctgggcgag acaccggccg tcgacgaagc cacgctcgcg atcatctccc ggttcttcgg
      481 ctcgcgcggc attaccatgc cggaacagaa caccaagcag gcctggctga tcccctcggc
      541 cgaggcgctg cccaacccga tgggcaccgc gcccggctgg ttcgtgcaac gtcagggacg
      601 attcatcgtg accatgccgg gcgtgccgcg cgagatgcgc cgcatgtggg ccgagcaagc
      661 cgttccccgg ctggcgccct cattgggcga ccaggtctac gtctcgcgca cgctgaagac
      721 gatcggcatc ggcgagtcgg ccgccgagaa ggagatcgcc gagatcatcc accggggcgg
      781 gccgatcgtg gccacctatg ccaaggacga cggggtgcac gtgcgtatcg tggccggcgc
      841 gccggagtcg ggcccggccc agcaagcggt tgcgcgtacc gtggccgagg tgcgcgccat
      901 cctcgggccg tacatctacg gcgagctggg cgtcagcctg ggctcggcca tcctggagcc
      961 gctgctgcgc accggcgcca cgctggcgct gagcgaggtc ggctccggcg ggcgcctgag
     1021 cgagctgctg gtcgaggagc cgaccgccga acgccacctg ctggccagcg tggtgcagcc
     1081 gtgggagcag gccgccgctc cgggcgagag cgtcgctgag gtggctgccc gcgaggcggt
     1141 caaggcgcgt gaccgcttcg gcgccacctt cggctacgcc atcgcggtgc gcatggagcc
     1201 gggcgagacg ccggacgtca cccgcggcca tgtggcgctc tgcctggccg gtcccggcga
     1261 gccggtcgag cgcgcccacc gcgtcaacgc catcccggcc gaggtccggc gccgggccgg
     1321 gctatgggcg gccgagttcc tctggaagac cctgcacgag caggtcgggg cagcctccgg
     1381 ctagctcggg gatcgcgcgc cggccacgcc gcaggccgca gcgccgcgcc cgccagatca
     1441 gccctgccgg gtagccgacc agcttggcca ggtcccccgc cagccgcagc gcgggcgcca
     1501 gcgccagcgc cggcagcatt tgcgagagcg gaaggcgttg ccgccacagt cgtagccacg
     1561 ggcggcgcat gtagaccgcg cccccggcga gcgccgcggc tgtcaggcgg cgatcacgcg
     1621 cgacgaacag cagcggcaac agcccgagat aggtgacgta gcgcagcgcg tggcggcgcg
     1681 cgaagagccc cgccttgccg tcgccgcggg cgtagcggaa gtactgccgc gtgtaggcgc
     1741 ccagcgtcgc acggggccgg aaccgggcga tggcttgcgg gcggaactcg aagcgcgcgc
     1801 cggccgcgcg cagccgcagg tcgaagacca aatcttcgca gtagtccagc cactcaggat
     1861 agcggaagcc cgcggcgatc agttcccgcc gcagcgccac ggagcggctg gagggcagga
     1921 agcgcgcggg ggcgatctcg ttcacgtccg gcagcgtgac cgcccccagc acgcgctcga
     1981 agcgggagca cggatcggcg cggaagaagc cggagacgac atccggctgc gcggccggcg
     2041 gccggtcgaa gggcgctacc agctcggcca gccagtccgg ctccagccgc acgccggcgt
     2101 cggtcaccgc cacgatggcg ccgcgcgccc ttgccagcgc gacgttccgg ccggccgcga
     2161 tgttcgcgcc gggcgccgcc agcaccgtca gcggcaggcg cccctgccag tccttcagcc
     2221 gcgccacggt gccgtcgtgc gagccgccat cgaccacgat gatctcgtcc ggtgacagtc
     2281 gctgcgccgc gatcgaggcc agcagcgcgt cgacggtggc cgcctcgtcc ttgacggtga
     2341 cgatcagcga gacgcgcggg cgctcaaacc cagtcacccg gcgactcccg ctcgaagtcc
     2401 gcgagtaagg cgtcctggcg cgccagcatg cgcgcgcgct cctcgggagt gaggtcgtca
     2461 aagccgagca gatgcaggaa gccgtggagc gccagatagg cgatctcgcg ctgcgtaccg
     2521 tgcccggcgc cgagcgcctg ctgggccgcg gtttcgaagg agacggcgat gtcgcccaga
     2581 taatcaccct cgccggggaa gctcatgaca tcggtcggac cgctcaaccc catgaactgc
     2641 tggtgcagcg cggcgatctc ggaatcgtcg cagacccaga gcccgacctc gccggccgca
     2701 gcctcctggc gcgcggcgaa gtcgagcagc gcggcaacgc ggtcggagtt gagtgagggc
     2761 gcgccggggt tcagccgaat ctcgacggag aaggccgggc cggcgctcaa tgggcggctt
     2821 ccaccggctg ccgtgccgcg gtcttggcca gctccgggta ctccacgcgc gggtgataga
     2881 tcccttcgag catggtgatg aaggtggcgc ggatctgctc gatctggcgc agcgtcaggt
     2941 cgcagtcgtc gagctggcgg tcctcaagcc gtgccttgat gacgccgtcc acgacctcct
     3001 tcagcccggt agaagcgcgc cgcccgtcgc cgttggcctc ctcgtagagc ttgccggact
     3061 gcgaggccgc gcgcaccgcc gcctcgacgc tgtcggacag catgatgatg gcggcctcct
     3121 tggtctgcgg ctttggcccc ttgtagcgga actcggcctc gtcgaccggc tcgcccgagg
     3181 cgagcgcctc ggcatagaag tacttgatca acgtcgtgcc gtggtgctgc tggatgacgt
     3241 cgatgatcgg ctgcggcagc cgcgccttgc gccccagccg cacgccgtcc tcgacgtgct
     3301 cctggatgat gcgggcgctg gtgcgcgggt cgagcgcgtc gtgcacattg gcacggttgg
     3361 cctggttctc gacgaagaag ctgggccgca gcaccttgcc gatgtcgtgg tagaggcagg
     3421 cgacacgggc gaagagcggg tcgccgccca ccgcctcgac cgcgctctcg gccaggttgc
     3481 tcaccacgat gctgtggtgg taggtgccgg gggcctcgcg cgccaggcgg tagagcagcg
     3541 gctggttggg gtgcgccagc tccagcagtt gcaggtgggt cacgatgccg aagacgcgac
     3601 cgagcatata gaagaagagg aacgtcaggc tggccgagac ggcgccgttg accgccgcgt
     3661 cacccaccaa ctgcccaacc tccgcggcgt cgacgtgccc gctgatcagc gcgagacaga
     3721 tggcggtggc gaagaccgcc agcgccaccc ccagcgccgc ccagaagaag cgcacgatgc
     3781 gctcggcgcg atagacgatc gccgcgccgg ccagggagct gacgaaggtc agtagggcga
     3841 cttcgaaggt gctccccgag acgatgccga ggtagagcgc caggaaaccg gccaggacca
     3901 ccgccagttg gaagtcgagc aagatcgaaa gcagcatggc cgcggccgcc gccgggaaca
     3961 tcaggcgcag ttggtcgctc tgcagcagga agcggccggt gacgaccggc accaccacga
     4021 tcagcgagat caagagaagc tggcgtagat cgtcggtgcc gctggcggtc ttgaagagat
     4081 agcccagcag cgccagcgtg acgagcgcca agaggccgag cgtgcccacg aattgcggcc
     4141 agttgtgcga cggggtcagc agcccgaagt gctctagctt ctcgatgtcg tacgaggtga
     4201 ccgggtcgcc gtcgcgcacg atcacctgcc cctgctgcac cgtgaccatg accgactgca
     4261 cgttcttggc cgcggcgtca cgcgcggcct tggtctgatc t
//
LOCUS       DASUBI010000781         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_686694, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000781 DASUBI010000000
VERSION     DASUBI010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686694"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="VFU72_15320"
     CDS             <1..1980
                     /locus_tag="VFU72_15320"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5424912.1"
                     /translation="ALEVAGQVSQHFPDGVGFVDLAPLTYPALALPTIAAALQVTEQG
                     DRTLLETLRQALAGRRLLLVLDNCEQILTAAPEIAQLLASTSTLSILATSRAPWRVRG
                     ERVFALEPLPVPDRDHRLPLAALAEAPSVALFVERARAARHDFALTEANAGAIAAICA
                     RLDGLPLALELAAARSKVLPPAALLARLEQRLPLLSDGARDLPARQRTLQQAIAWSYD
                     LLPLPEQTLFRRLGVFVGGWTLESAEAVANHDDRLDIIAGLGSLVDKSLIRQTEQPDG
                     EPRFTMLETIREFAAEQLAASGEGEDARSRHARFVVGMVERAEPELEGSGQAAWLDRL
                     DAEHDDLRAAMVWAARHDGMLLARLAVAAAPFWSIRGYLTEGRTWLETALADRDVTPA
                     VRAQILLAAGSVARRQCDWPRATALFAEALAAWQDLGDAVRIAEAELGLGLVTAECGD
                     AEAAVVHYEAALARFRQLGDLHGVAACINSLASIALDRGEYAQAQNLYEEAIALQRSV
                     GNERRRSRSLGALGHVAYAQGDLPRALALYRDSLLGCWAHRDAMGIEGLFATLASVGL
                     DHDHATRATHLLGAAEAVREAAGLGMTEPDRAEYERLAAKARQALGAGAWSTAWAAGR
                     AMPREEAVAAALALADELGRDASTESEETVVRTGG"
BASE COUNT          347 a          766 c          825 g          356 t
ORIGIN      
        1 gcgctcgagg tggcgggtca ggtcagtcag cacttccccg acggcgtcgg cttcgtcgat
       61 ctggccccgc tgacctaccc ggccctcgcc ctgccgacga tcgccgccgc gctgcaggtg
      121 acggagcagg gtgaccgtac cctgctggag acgctgcggc aggcccttgc cggccgccgc
      181 ctgttgctcg tcctcgataa ttgcgagcag atcctcacgg ctgcgccaga aatcgcccag
      241 ttgctcgcaa gcacgtccac gctgtcaatc ctggcgacca gccgggcccc gtggcgcgtg
      301 cgtggcgaac gcgtcttcgc gcttgagccg ctccccgtgc ccgaccgcga tcatcggctg
      361 ccgctggcgg cgctcgccga ggcgccgtcc gtcgcgctct ttgtcgagcg agccagggcg
      421 gcccgccacg atttcgccct caccgaggcc aatgccggcg ccatcgccgc tatctgcgcc
      481 cggctcgacg gcttgccgct tgccctggaa ctggccgctg ctcggagcaa ggtgctcccg
      541 cccgccgcct tgcttgcgcg cctggagcag cgactccccc tgctgagcga cggcgcgcgg
      601 gacctgccgg cgcggcagcg cacgctccaa caggcgatcg cctggagtta cgacctcctc
      661 ccattgccgg agcaaaccct cttccgacgg cttggcgttt ttgtcggcgg ctggacgctc
      721 gagtcggcgg aggcggtggc taatcacgac gacaggctgg acatcattgc aggactgggt
      781 tcgttggttg acaagagcct gatccggcag accgagcaac cggatggcga accacgattt
      841 acgatgcttg agaccatccg cgagttcgcc gccgaacaat tggcggcgag cggcgagggc
      901 gaggatgctc gatcccgcca cgcgcggttc gtggtgggca tggtggagcg agcggaaccc
      961 gaactggagg gatcgggcca ggcggcctgg ctggaccggc tggacgccga gcacgacgat
     1021 ctgcgggcag cgatggtctg ggcggcccgg cacgatggga tgctgctcgc gcgcctcgct
     1081 gtcgccgccg ctcccttctg gtcgatccgc ggctacctga ctgagggccg gacgtggcta
     1141 gagacggcgc tcgcggaccg cgacgtgacg ccagctgtcc gggcgcagat tctactggcg
     1201 gcagggtctg tggcgcgacg acagtgcgac tggccgcgcg cgaccgcgct attcgcggag
     1261 gcgctggcgg cctggcagga tcttggcgat gcggtccgca tcgcggaggc cgaacttggc
     1321 ctcggcttgg tgacggcgga atgcggcgac gccgaggcgg cggtcgtcca ctacgaggcg
     1381 gcgctggccc ggtttcggca gttaggcgat ctccacggag tcgcggcctg catcaacagc
     1441 ctggccagca tcgctcttga tcggggggag tacgcgcagg cccagaatct ctatgaagag
     1501 gcgatcgcgc tgcaacgatc ggtggggaat gagcgtcggc ggagccgctc tcttggagcg
     1561 ctgggccatg tggcctatgc gcagggtgat ctcccgcggg cactggcgtt gtatcgagac
     1621 agcctcctcg gatgctgggc gcatcgcgac gcaatgggca tcgaggggct gttcgccacc
     1681 ctggcaagcg tggggctgga ccatgaccac gccacgcgag caacgcacct gcttggggca
     1741 gcggaggcgg tgcgagaggc cgccgggctc gggatgacag agccggaccg agcggagtac
     1801 gagcgcctcg ccgccaaagc gcggcaagcg ctcggcgcgg gggcgtggtc aacggcctgg
     1861 gcggctgggc gtgcgatgcc acgggaggag gcggtcgctg cggcattggc gctggccgac
     1921 gaactgggtc gggatgcctc aaccgaatcg gaggagaccg tcgtcagaac cggcgggtga
     1981 tcggccgtcg ccgcgctggc aaccatgccg cggcgcgacc tacgcaggac gatgccggcg
     2041 cccgacggtt gtggcatatg ccgcgctcct tagcgcgggc tgggatcgca acccggcgct
     2101 cggctccttg agtcgcccgt tcggtcgtcg caaaacttcg cagctatcgc aagcactgcg
     2161 acgttgattc tgagacgact tttgcaacgc cagaatccgt gcaaacctcc gcacaacccg
     2221 accccgcgcg acccgtctca caaagggtcc agcctgtgga aaaagaggaa caccgctggg
     2281 cgccggagga gcgg
//
LOCUS       DASUBI010000782         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_974026, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000782 DASUBI010000000
VERSION     DASUBI010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974026"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1157)
                     /locus_tag="VFU72_15325"
     CDS             complement(141..1157)
                     /locus_tag="VFU72_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5424913.1"
                     /translation="MPSTSGAAPSLAAFQNAITSVVDKAKPAVVEITNEQTSIAQLSQ
                     PIPVGTGSGFIFDGDGHVLTNNHVVEGAESLVVTLPDGRSFKDKDVKIVGTDPLTDLA
                     VLKINGGNLPVIALGDSTQLQVGEWLVAMGYALGLEGQPSVTAGVVSALGRTVQEPGE
                     GRNTLGPYLFDVIQTDAAINPGNSGGPLTNLNGEVVGINTLIAVEAEPGVQAQGIGFA
                     IAINTAKKIADEIIKTGRAVHPFVGVQYVPLTPAIAAQLNTSAKQGVLIQSVIAGSPA
                     AHAGLQQGDVVTEVDGKALQTDSSFAEAIESHGVGDVITLTVLRDGQSQQIPVTLGQR
                     PQGS"
     gene            1147..1437
                     /locus_tag="VFU72_15330"
     CDS             1147..1437
                     /locus_tag="VFU72_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424914.1"
                     /translation="MLGTVVAAAADVVPVAAAAAVPGMLVALPTAPVAFAAAVAGREA
                     LGEDDGKVGTAVPLDPLSEQAAPTISSSVTAQSRWPNRRLGWRRIASSRLVR"
     gene            1610..>3434
                     /locus_tag="VFU72_15335"
     CDS             1610..>3434
                     /locus_tag="VFU72_15335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5424915.1"
                     /translation="MTGRAASSEAQPLTAEQLVSWTRPSDARISPDGQRVAFARKPVS
                     KEGEHPESSIWLLPFEDGAPVRFSGGLWNDDAPRWSPDGNHLAFLSDRAERGKQSVYV
                     MPVGGGEARRVFEQQGELAELAWSPDGRYLSVLFTEPETEEEKKRKEERDDAHEWNAD
                     WKFRRLWVIDLSQPDGEANPPARVISPKAQQVWTYCWSPDSARLAIDTTSSPLLDEMF
                     RPHEVALVGVDGGEPSPLFTVQGPAHNLTWSPDGTRLAYLAPGARVVHGEYVYSRPVA
                     GDEPVCLTPHYPGTADFIGPLGDDLLLLAYEGVDAVLYRLGWDGTRSPLLARQPGGRI
                     AAPVTASVDGRRIALVREDASHAPNVWVADLSVSPAELTPRTQFHPELEEAALGQPHV
                     IRWTSDEGVEVEGILFKPHGYQAGQRYPLVVQVHGGPTHHWSNQFAATWHDWAHYLAG
                     RGYAVLLPNPRGSTGRGPAYSNAIFGDVGGGEFRDMMSGVDHLIAAGLADPDRLGVGG
                     WSWGGYMTAWTVSQTGRFKAAVMGAGLANMVSDNSLGDIPTANLSYFEESPYRDPLAY
                     FERSAIRYIHQASTPTLILHGAEDKRVHPSQGVEMYVALRTL"
BASE COUNT          501 a         1171 c         1222 g          540 t
ORIGIN      
        1 agtagacctg atgcccgtcg ccggtgtcga gcatgccgct ggcgtacggc tcaatggggg
       61 gaaactgatc agccatcgtt ctcgctttcg ccggacgtgg cgggcgcggt gtcgtgatac
      121 gacctccgcc cccgccgtca tcaggatcct tgtgggcgct gacccagcgt caccgggatc
      181 tgctgcgact ggccgtcgcg cagcaccgtc agggtgatca cgtcgccgac gccgtggctc
      241 tcgatcgcct cggcgaagga ggaatcggtc tggagcgcct tgccgtcgac ctcggtgacc
      301 acgtcgccct gctgcaggcc ggcgtgcgcc gccggggagc cggcgatcac gctctggatc
      361 agcacgccct gcttggcgct ggtgttgagc tgcgccgcga tggccggcgt cagcggcacg
      421 tactgcacgc cgacgaaggg gtggacggcc cgcccggtct tgatgatctc gtcggcgatc
      481 ttcttggcgg tgttgatcgc gatcgcgaag ccgatgccct gggcttgcac accgggctcg
      541 gcctcgacgg cgatcagcgt attgatgccg acgacctctc cgttgagatt agtcaagggg
      601 ccgcccgagt tgccgggatt gatggcggcg tcggtctgaa tgacgtcgaa gagatagggg
      661 ccgagcgtgt tccgtccctc gcccggctcc tgcaccgtgc gccccagcgc gctgaccacc
      721 cccgcggtca ccgagggttg accctccagc ccgagcgcgt agcccatggc gaccagccac
      781 tcgcccacct ggagctgggt cgagtcgccc agcgcgatca ccggcaggtt gccgccgttg
      841 atcttgagca cggccaggtc ggtcagcgga tcggtgccga cgatcttgac gtccttgtcc
      901 ttgaaagagc ggccgtcggg cagggtcacg accaggctct cggcgccctc gaccacgtgg
      961 ttgttggtca ggacgtgccc gtcgccgtcg aagatgaagc cggagccggt accgaccggg
     1021 attggctgcg agagttgggc gatgctggtc tgctcgttgg tgatctccac caccgctggc
     1081 ttggccttgt cgaccaccga ggtgatggcg ttctgaaagg ccgccagcga gggagccgcc
     1141 ccgctggtgc tcggcacggt cgtggccgcg gccgcggatg tcgtgccggt ggccgcggcc
     1201 gccgcggtgc ccgggatgct cgtcgcgctg ccgacggcgc cagtcgcgtt cgccgccgcc
     1261 gtcgcgggcc gagaggcgct cggcgaggat gacggcaagg tcggcaccgc ggtgccgctc
     1321 gatccgctga gcgagcaggc cgcgccgacg atcagcagca gcgtgacggc gcagagtcgc
     1381 tggcccaatc gccggctcgg gtggcggcgc atcgcttcct ccaggctagt caggtagctc
     1441 gtcgcttcgg ctcgagttcg ggcaattatc catcgtggcg caaggacggc aacggggccg
     1501 gcgcgcgagg gcctgctgag aagcggtacg gctggcgtag aatgccgcca accactcaga
     1561 gccgcccgtg cgcggcgcga ccgcgtgaca ccgtgtggaa ggagctcccg tgaccggaag
     1621 agccgcctcg agcgaggcac agccgctgac tgccgagcag ctcgtctcct ggacgcgccc
     1681 cagcgacgcg cgcatctcgc cggatggcca gcgggtcgcg tttgcccgta agccggtcag
     1741 caaggaaggt gagcaccccg agtcgtcgat ctggctgctg cccttcgagg acggcgcgcc
     1801 ggtgcgcttt agcggcggcc tctggaacga tgacgcgccg cgctggtcgc cggatgggaa
     1861 ccatctggcc ttcctctccg accgcgccga gcgcggcaag cagagcgtgt atgtgatgcc
     1921 ggtcggcggc ggcgaggcgc ggcgcgtctt cgagcagcag ggcgagctgg ccgagctggc
     1981 ctggtcgccg gacgggcgct acctctcggt cctcttcacc gagcccgaga ccgaggaaga
     2041 gaagaagcgc aaggaggagc gtgacgacgc gcacgagtgg aatgcggact ggaagtttcg
     2101 ccgcctctgg gtgatcgacc tgagccagcc ggacggcgaa gcgaacccgc ccgcacgcgt
     2161 catatcccca aaagctcaac aggtctggac ctactgctgg tcccccgaca gcgcgcggct
     2221 ggcgatcgac accacgtcgt cgccactgct ggacgagatg ttccgcccac acgaggtggc
     2281 gctggtgggg gtcgacggcg gcgaacccag cccgctcttc actgtgcaag ggccggcgca
     2341 caacctgacc tggtcgccgg acggcacgcg gctcgcctat ctcgcgccgg gcgcgcgcgt
     2401 ggtccacggc gagtatgtct actcccgtcc ggtggccggc gacgaaccgg tctgcctcac
     2461 gccgcactac cccggcacgg cggacttcat cggcccgctt ggcgatgacc tgctgctgct
     2521 ggcctatgag ggcgttgacg cggttctcta ccggctcggc tgggacggca cgcgctcgcc
     2581 gttgctggcc cggcagcccg gcgggcgtat cgcggcgccg gtcactgcct cggtcgacgg
     2641 ccggcgtatc gcactggtgc gcgaggacgc cagtcacgcg ccgaatgtct gggtggccga
     2701 tttgtccgtg tccccggccg agttgacccc gcgtacgcag ttccaccccg agctggagga
     2761 agccgcgctg ggccagccgc acgtgatccg ctggaccagc gacgaggggg tcgaggtcga
     2821 aggcatcctc ttcaagccgc acggctatca ggcgggacag cgctacccct tggtggtgca
     2881 ggttcacggc gggcccaccc accactggag caaccagttc gccgccacct ggcacgactg
     2941 ggcgcactac ctggccgggc gcggctacgc cgtgctgctg cccaacccgc gcggcagcac
     3001 cggccgtggg ccggcctaca gcaacgccat cttcggcgac gtcggcggcg gcgagttccg
     3061 cgacatgatg tccggcgtcg atcacctgat cgcggccggg ctggccgatc cggatcggct
     3121 cggggtcggc ggctggagct ggggcggcta catgaccgcc tggaccgtca gccagactgg
     3181 acgcttcaag gcggcggtga tgggcgccgg gctggcgaat atggtcagcg acaacagcct
     3241 gggcgacatt cccacggcca acctctccta cttcgaagag agtccctatc gcgatccctt
     3301 ggcctacttc gagcgctcgg ccatccgcta catccaccag gccagcacgc cgacgctgat
     3361 cctgcatggc gcggaggaca agcgggtgca cccgagccag ggggtggaga tgtacgtggc
     3421 gctgcgtacg ctgg
//
LOCUS       DASUBI010000783         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_400311, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000783 DASUBI010000000
VERSION     DASUBI010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400311"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFU72_15340"
     CDS             <1..1173
                     /locus_tag="VFU72_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5424916.1"
                     /translation="LPVSLKDLTVTKGIRTTRGSLLTADWVPEDDAPVTERVYAAGGV
                     LLGKTNTPESGWKGESTNRVVGSSQNPWQIGRTPGGSSGGAGAAVAAGLGQLAQGTDG
                     AGSIRIPASFCGIFGLKPSYGLVPYWPGSAVQSIAHVGPMTRTVRDGALLLDALAGPD
                     PRDRGAIPPAGNYLAACEGGVAGLRLGWAADLGFGRAEPAVEAAVEEAARVFQELGAT
                     VEPVAPGWSDPFPIIDALWSSGMAASRPDFEQVRTLLDPGLARVIEHGLELRGVDVAR
                     AEVLRTQFYEDVRRFTAPFDLLLTPTVPVTAFAAGRDDPGEINGQARSYLGWTYFTYP
                     FNLTGQPAATVPAGFVDGLPVGLQLVGRFRDDATVLRAAAAFESVRPWAAERPSLG"
     gene            1274..1813
                     /gene="tsaE"
                     /locus_tag="VFU72_15345"
     CDS             1274..1813
                     /gene="tsaE"
                     /locus_tag="VFU72_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5424917.1"
                     /translation="MPSHRPALDVISHSPDQTRQIGYRLGRLARPGDIYLLSGPVGGG
                     KTTFVQGLARGLGVRGYVQSPTFALAMEHAASLADGAPATLYHLDFYRLEGAQELETF
                     GYQEYLEAPEGIVAVEWPERLALALPDTYLLVSFEHLAEAKRRLLFRPHGSRAQELVN
                     ALREEVTGAPRRSAPAGDR"
     gene            1776..2468
                     /gene="tsaB"
                     /locus_tag="VFU72_15350"
     CDS             1776..2468
                     /gene="tsaB"
                     /locus_tag="VFU72_15350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5424918.1"
                     /translation="MPHDGRLLLAIDSSTEIAGLALYDGDSVSELSWNAGRHQTVSLI
                     PEVDQLLRLNGRALADLHAVAVAVGPGSFNGLRVGLSVAKGLAYALGLPLVGVGTLDA
                     TAYPHTGTRAPIRALVLAGRGRAVFADFRYRNGTWRRLSELRNVPVAELAEGLTERTV
                     IVGDMGAPLASELASVPLAVVPPPALRARRPAYLAEIGFRRWQAGDLDVLEALEPVYV
                     HGVAPGTPAAAS"
     gene            2488..3165
                     /gene="rimI"
                     /locus_tag="VFU72_15355"
     CDS             2488..3165
                     /gene="rimI"
                     /locus_tag="VFU72_15355"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5424919.1"
                     /translation="MERDATVENGNGAELERFELDPMRIEDVSEVSRVEHRCFTNPWP
                     ESAYRRELRNPEGNYYIVLRHVEPESAPSTVEPEPRGRLSLLPLIRRGERPSTHPIVG
                     FAGMWFLYDESHVTTIGVAPELRGHGLGEVLLVSLFDEALRRNAQWVTLEVRVSNSPA
                     QALYEKYGFTRQGVRRRYYSDNGEDAYIMWSPSLRDPDYLRQFQTLKSELRARLTREA
                     IVVETSL"
     gene            3165..>4214
                     /gene="tsaD"
                     /locus_tag="VFU72_15360"
     CDS             3165..>4214
                     /gene="tsaD"
                     /locus_tag="VFU72_15360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5424920.1"
                     /translation="MVILGIETSCDETSAAVVNRELRILANVVLSQTELHQRYGGIVP
                     ELASRRHITSIIPTIELALEQAGVVREDLDAISVTEGPGLAGSLLVGVNVAKTLAYAW
                     GKPLVPVNHLEGHIYANWLTQDDAPLTPPPTFPLVCLVASGGHTELLLMRGHGEYELL
                     GRTLDDAAGEAFDKSARLLNLGYPGGPAIQNAAAQGRTGQHPMPRAWLGESYDFSFSG
                     LKTAVLRTTEQYRRPAPRGRSQRASGNEPFQPHQPVELNPNMPVNDIAADVQEAIVDV
                     LAEKTARAARETAATMVLLAGGVAANALLRKRLEQLVDVPLRYPPLILCTDNAAMIAG
                     AAHFALARGEVAPLTL"
BASE COUNT          598 a         1437 c         1536 g          643 t
ORIGIN      
        1 ctaccggtct cgctcaaaga cctgaccgtc accaaaggaa tccgcaccac ccgcggctcg
       61 ctgctgaccg ccgactgggt gccggaggac gacgcgccgg ttaccgagcg cgtctacgcg
      121 gcgggcggcg tgctgctggg caagacgaat acgccggagt cgggctggaa gggtgagtcg
      181 accaaccgcg tggtcgggtc gagccagaac ccctggcaga tcgggcgtac gccgggcggc
      241 tccagcggcg gggcgggcgc ggcggtcgcc gccggcctgg gccagttggc gcagggtacc
      301 gacggcgccg gctcgatccg catcccggcc tccttctgcg gcatcttcgg tctgaaacca
      361 tcctacggcc tggtccccta ctggccgggc agcgcggttc agtccatcgc ccacgtcggc
      421 ccgatgaccc gcaccgtgcg cgacggcgcg ctgctgctcg acgcgcttgc cggccccgac
      481 ccgcgggacc gcggcgcgat tccgcccgcc ggcaactacc tggcggcctg tgagggcggc
      541 gtggccggtc tgcgcctcgg ctgggcggcg gacctcggct tcggacgggc cgagccggcc
      601 gtcgaggcgg cggtggagga ggcggcgcgc gtgttccagg agctgggtgc gaccgtcgag
      661 ccggtcgcgc caggctggtc cgatcccttc ccgatcatcg acgcactctg gtccagcggg
      721 atggcggcca gccggccgga cttcgagcag gtgcgcacgc tgctcgaccc gggcctggcg
      781 cgcgtcatcg agcacgggtt ggagctgcgc ggcgtcgacg tggcgcgcgc cgaggtgctg
      841 cgcacgcagt tctacgagga tgtgcgccgc ttcaccgcgc ccttcgacct gctgctgacc
      901 ccaacggtgc cggtgacggc ctttgccgcc ggccgggatg accccggaga gatcaacggg
      961 caagccagga gctatctggg ctggacctac ttcacctatc ccttcaacct gaccgggcag
     1021 ccggcggcca ccgtgccggc cggcttcgtc gatgggctgc cggtgggctt gcagctcgtc
     1081 ggacgcttcc gcgacgatgc cacggtgctg cgagccgcgg ccgccttcga gtcggttcgg
     1141 ccctgggcag cggagcgtcc gtcgctcggc tagccttgcc gggtgtgcgc caatccgtct
     1201 atcatgcggc tcggcgcagg cgacctgcgc gtcggggtgg ggtgctcggc cggcaaggtg
     1261 agaggtgagc ggaatgccgt cgcaccgtcc ggcgctcgac gtcatcagcc atagtccgga
     1321 tcagacgcgg cagatcggct atcgtctcgg gcgcctggcg cgtcccggcg acatctacct
     1381 cctctcgggt ccggtcggcg gcggcaagac gaccttcgtg caggggctgg cccgcgggct
     1441 gggggtgcgc gggtatgtgc aaagcccgac gttcgccctg gcgatggagc atgctgccag
     1501 cctggccgac ggcgcgccgg ccaccctcta ccaccttgat ttctaccgtc tggagggggc
     1561 tcaggaactg gaaaccttcg gctaccagga gtatctggag gcgcccgagg ggatcgtggc
     1621 ggtggagtgg cccgagcgac tggcgctggc gctgccggac acctacctgc tggtttcgtt
     1681 cgagcatctg gccgaggcca agcgccggct cctcttccgt ccacatggct cccgtgctca
     1741 ggagttggtg aacgcgctcc gcgaggaggt cactggtgcc ccacgacggt cggctcctgc
     1801 tggcgatcga tagctcaacc gagatcgcgg ggctggcgct ctacgacggc gacagcgtca
     1861 gcgagctgag ctggaatgcc gggcggcacc aaaccgtttc cttgataccg gaggtggatc
     1921 aacttctgcg cttgaacggg cgcgccctgg ccgacctgca cgcggtggcg gtggcggtcg
     1981 ggccggggag cttcaatggc ctgcgcgtgg ggctgagcgt ggccaagggg ctggcctacg
     2041 cgctcgggtt gccgctggtc ggcgtgggca cgctggacgc gacagcctac ccgcacactg
     2101 ggacgcgggc gccgatccgc gcgctggtgc tggcgggccg tggtcgcgcg gtcttcgccg
     2161 acttccgcta ccgcaacggc acctggcggc gcctcagcga gctgcgcaac gtgccggtgg
     2221 ccgagctggc ggaggggctg accgagcgga cggtgatcgt gggcgacatg ggcgcgccgc
     2281 tggcgagcga attggcgtcg gtgccgttgg ccgtcgtgcc gcccccggcg ctgcgcgcgc
     2341 ggcgcccggc gtatctggcc gagattggct tccggcgctg gcaggcgggc gacctggacg
     2401 tgctggaggc gctggagccg gtctacgtgc atggcgtggc gccggggaca ccggcggcgg
     2461 ctagctagcc gggtgtcgag atctgggatg gagcgggacg cgacggtgga gaacggcaac
     2521 ggagcggaat tggagcgttt cgagctcgat ccgatgcgca tcgaagacgt gtcggaggtc
     2581 tcacgcgtcg aacaccgctg cttcacgaat ccctggccgg agtcggccta ccggcgcgag
     2641 ctgcgcaatc ccgaggggaa ctactacatc gtgctgcgcc acgtcgagcc ggagagcgcg
     2701 ccatccaccg tcgagccgga gccgcgcggt cggctctcgc tgctgccgct gatccggcgc
     2761 ggcgagcggc cgagcaccca cccgatcgtc ggcttcgccg gcatgtggtt cctctacgac
     2821 gagagccatg tcaccaccat cggcgtggcg cccgagctgc gcgggcacgg gctgggcgag
     2881 gtgctgctgg tgtcgctctt cgatgaggcc ctgcgccgca acgcccagtg ggtgacgctg
     2941 gaggtgcgcg tctcgaacag cccggcccag gcgctctacg aaaaatacgg cttcacccgg
     3001 cagggcgtgc gccggcgcta ctacagcgac aacggcgagg acgcctacat catgtggtcg
     3061 ccctcgctgc gcgatcccga ctacctgcgg cagttccaga cgctcaagag cgagctgcgc
     3121 gcgcgtctga cgcgcgaggc gatcgtggta gagacgagtc tctgatggtg attctaggca
     3181 tcgaaacctc gtgcgatgag acctcggccg cggtcgtcaa tcgtgagctg cgcatcctcg
     3241 ccaacgtggt gctctctcag accgagctgc accagcgcta cggcggcatc gtgcccgagc
     3301 tggcctcacg gcgccacatc accagcatca tccccaccat cgagctggcg ctggagcagg
     3361 cgggtgtggt gcgcgaggac ctcgacgcca tctctgtgac cgaaggccca gggctggccg
     3421 gctcgctgct ggtcggggtc aacgtggcca agacgctggc ctatgcctgg ggcaagccgc
     3481 tggtgccggt caatcacctg gagggccaca tctacgccaa ttggctgacg caagacgacg
     3541 cgccgttgac cccgccgccg accttcccgc tggtctgcct ggtcgcctcc ggcggtcaca
     3601 ccgaactgct cctgatgcgc gggcacggcg agtatgaatt gctgggccgc acgctcgacg
     3661 atgccgccgg agaggcattc gacaaaagcg cccggctact caacctgggc tacccgggtg
     3721 gcccggcgat ccagaacgcg gcggcccagg gccgcaccgg gcagcatccg atgccgcgcg
     3781 cctggttggg cgagagctat gacttcagct tcagcggact caagacggcg gtgctgcgga
     3841 ccaccgaaca gtatcgccgc ccagcaccgc gcggccggag tcaacgcgcg tcgggcaacg
     3901 agccgttcca gccgcatcag ccggtggagc tcaaccccaa catgccggtc aacgacatcg
     3961 ccgccgacgt gcaggaggcg atcgtggacg tgctggccga gaagacggcg cgagcggccc
     4021 gcgagacggc ggcgacgatg gtgctgctgg ccggtggcgt ggcggccaac gcgctgctgc
     4081 gcaagcggct tgagcaactg gtcgacgtgc cgctgcgtta tccgcccctc atcctctgca
     4141 ccgacaacgc ggccatgatc gccggtgcgg cgcacttcgc actggcacgg ggcgaggtcg
     4201 ctccgctgac gctc
//
LOCUS       DASUBI010000784         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_701497, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000784 DASUBI010000000
VERSION     DASUBI010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701497"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFU72_15365"
     CDS             <1..1149
                     /locus_tag="VFU72_15365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5424921.1"
                     /translation="GEIELHVFPLRYPLRRGRYSVYGATVHPQGGGVTRQAGRGLLLA
                     RALAALAAEHRRGRFDLLHAFWADEPGALAVTAAPLLRVPVLVSLAGGELACFPEIGY
                     GGQLHRSSRALTRLALRRAAAVTAGSNYLRELATPLADRVELLPLGVDVELFGDDVPS
                     PPVGTSHPQLFSPAGEKGGGDSVGVGFKPALPRPASFDPNCVHVLQVASLVAVKQPGM
                     LLEAFARAAQVCPALRLHLVGDGPLEEALRAQSDRLGITGQVCWHGAVAHEALPAYYR
                     AADFCLLASRHEAEGMVVLEAAAAGRVTAGTRVGLVPELAPGHSVAVDDVAGLASVMI
                     ALASDAGLRTALGRQAREIVRERYSLSHTASQALALYRELAAGARRGA"
     gene            complement(1110..>2141)
                     /locus_tag="VFU72_15370"
     CDS             complement(1110..>2141)
                     /locus_tag="VFU72_15370"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU5424922.1"
                     /translation="FFWLRVGAAAALVLLLSVAVLIAPRALLPLDRALLILALAGVVA
                     TALSGAVSAILQATGSFGRLALVMLTNALLTALLALGLRAFDLLTLTSALVVLGIGTS
                     LASFAVGRRLLPGGMGLSLRPPTRALPELRAESRSLLGFGLWVWLANSLAMLTTQLDL
                     LLAAHWLSPAALGSYALAGNLAGKVDVINQSLHAALLPAASSLSTRARVRAYLRRGLV
                     PGALVAALVLILAPFARPLILFVFGTAYRDAVALFLALLVVAVFDVFTAPLLLLPFAA
                     RRPRLLAAADGLRALTLLVAAVWLVPPFGAFGLVAAKFLSRLAGLLLTVVVLAARYAP
                     RRAPAASSR"
BASE COUNT          322 a          741 c          830 g          248 t
ORIGIN      
        1 ggcgagatcg agctgcacgt ctttccgctg cgctaccccc tgcgccgcgg ccgctactcg
       61 gtctatggcg ccacggtgca cccgcagggc ggcggcgtca cccggcaggc cggccgaggg
      121 ctcctgctgg cgcgcgcgct ggcggcgctg gccgccgagc accggcgcgg ccgcttcgat
      181 ctcctgcacg ccttctgggc cgacgagccg ggggcgctgg cggtgacggc cgcgccgctg
      241 ctgcgcgtgc ccgtgctggt ctcgctggcg ggcggtgagt tggcctgctt ccccgagatc
      301 ggctacggcg ggcagctcca ccgcagcagt cgcgcgctca cgcgcctggc gctgcgccgc
      361 gccgccgccg tgacggccgg ctcgaactac ctgcgagagc tggctactcc gctcgcggat
      421 cgggtcgagc tgctgccgct gggggtggat gtggagctgt tcggggatga cgttccctca
      481 ccccccgtgg ggacctctca tccccagctc ttctctcccg cgggggagaa gggaggcgga
      541 gactcggtag gggtgggttt caaacccgcc cttccccgac cggctagctt cgatcccaac
      601 tgcgtgcatg tgctgcaggt ggcgtcgcta gtggcggtga aacagccggg gatgctgcta
      661 gaggcgttcg cgcgggcggc gcaggtatgc ccggcgctgc ggctgcatct ggtgggcgat
      721 ggtccgctgg aggaagcgct gcgcgcgcag tccgaccggc tcggcatcac cgggcaggtc
      781 tgctggcacg gcgcggtggc gcacgaagcg ctgccggcct actaccgcgc ggctgacttc
      841 tgcctgctcg cttcgcgcca cgaggcagag gggatggtcg tgctggaggc ggcggcggct
      901 ggccgggtca cggccggcac gcgcgtgggc ctcgtgccgg agctggcgcc cggccacagc
      961 gtggcggtcg acgatgtggc ggggctggca tcggtgatga tcgcgctggc aagcgacgcc
     1021 gggctgcgca cggcgctggg ccggcaggcg cgggagatcg tgcgggagcg ctacagcctt
     1081 tcccacacgg cgagccaggc gctggcgctc tatcgcgagc tagcggccgg ggcgcggcgt
     1141 ggagcgtagc gcgccgccag gacgactacg gtcagcagca gcccggccag gcgcgacagg
     1201 aacttcgccg ccaccaggcc aaaggcgccg aagggcggca ccagccagac ggccgccacc
     1261 aggagggtga gggcgcgcaa cccatcggcc gccgccagca gccgtggacg gcgcgcggcg
     1321 aaggggagca gcagcaaggg cgcggtgaag acatcgaaga cagccaccac cagcagcgcc
     1381 aggaagagcg cgaccgcgtc gcggtaggcc gtgccgaaga cgaagaggat cagcgggcgc
     1441 gcgaaggggg ccagaatcag gacgagcgcc gccaccagcg cgccggggac gaggccgcgc
     1501 cgcagatacg cgcgcacgcg cgcgcgcgtg ctgagcgacg aggcggccgg cagaagcgcg
     1561 gcgtgcaggc tctggttgat cacgtcgacc ttgccggcca ggttgccggc cagcgcgtag
     1621 ctgccgagcg cggccggcga gagccaatgc gccgccagca gcagatccaa ttgggtcgtc
     1681 aacatcgcca ggctgttcgc cagccagacc cagaggccga aaccgaggag tgaccgagat
     1741 tccgcgcgca gctcgggtag cgcgcgcgtc ggtggccgta ggctcaggcc catgccgccg
     1801 ggcagcaagc gacgcccaac cgcgaagctc gccagcgagg tgccgatgcc gagcacgacg
     1861 agcgcgctgg tcaaggttag caggtcgaac gcgcgcagcc cgagcgccaa cagcgcggtc
     1921 aagagcgcgt tggtcagcat caccagcgcc aggcggccga agctgccggt ggcctggagg
     1981 atcgcactga ccgcaccgct cagcgccgtg gccaccacgc cggccagcgc caggatcagc
     2041 agcgcgcggt cgagcggcag cagcgcgcgg ggcgcgatga gcaccgccac cgagagcagg
     2101 agcacgagcg cggcggccgc gcccacgcgc agccagaaga a
//
LOCUS       DASUBI010000785         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_225018, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000785 DASUBI010000000
VERSION     DASUBI010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225018"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFU72_15375"
     CDS             complement(<1..403)
                     /locus_tag="VFU72_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424923.1"
                     /translation="MTPVIVAGVFYLAVVALCLARPHAGRLFVGVFFWVMALGVHGYF
                     IIANPQSYVDFAHSAYLALYRDLAGPLVELSPRGFGLLCLVFELAVGTLILSKGRAVR
                     LGLLAGIAFLLAITPLGKEELINPLLAAGLAS"
     gene            complement(571..723)
                     /locus_tag="VFU72_15380"
     CDS             complement(571..723)
                     /locus_tag="VFU72_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424924.1"
                     /translation="MLELVVLDSSNPIIVIPALLSGFLVNPAWYLWLGLALRREQPAQ
                     TGAAGN"
     gene            875..1636
                     /locus_tag="VFU72_15385"
     CDS             875..1636
                     /locus_tag="VFU72_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEU5424925.1"
                     /translation="MGEGSGAIDGEGTPDLSVVVPAYNERGCIAASLAAMSAYLDSLA
                     LDYEIIVVCDGSQDGTEQIVAALACQHCRLRLISYPCNRGKGFAVRTGITAARGRFVM
                     FFDADLTVPITIVPQFLAALRDGYDIAIASRRHPASSALALLPRRRQVMGQVFSWFVR
                     RLVIDDLGDTQCGGKAYRAEVARCLFGRQRIDHFSFDAEVLYLARRLNCRIKEVPFVI
                     QHAPSGSSIQPVRDSLVMLRDLLRIRLYALRGRYE"
     gene            1738..>2294
                     /locus_tag="VFU72_15390"
     CDS             1738..>2294
                     /locus_tag="VFU72_15390"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5424926.1"
                     /translation="MTAGVNAGVIEAHRAGLVGSASLMVTTPAFDGALELARAHPALD
                     LGIHLALTGVPPALAHDAASGLVAGDRFHSLGVLLARLASGRVSAPALRAELRAQLER
                     ALASGLTFTHLDSHHHVHLLPHISTVVGELARECGIAFVRRVPALEAGTPCRPAQVLK
                     RLMLRGADWHAQPALSGLRHADAFRG"
BASE COUNT          354 a          796 c          800 g          344 t
ORIGIN      
        1 gagaggcgag accggcggcc agcaggggat tgatcaactc ctccttgccc agcggggtga
       61 tcgccagcag gaacgcgatg ccggccagca gaccaagcct caccgcgcgg cccttgctca
      121 ggatcagcgt gccgaccgcc agctcgaaga cgaggcagag caggccgaag ccgcgcgggc
      181 tgagctcgac cagtgggccg gcgaggtcgc ggtagagggc gagataggcg ctgtgggcga
      241 aatccacgta gctttgcggg ttggcgatga tgaagtagcc atgcacgccc agcgccatga
      301 cccagaagaa aacgccgacg aacaatcgac ccgcgtgcgg ccgcgccagg cagagcgcga
      361 ccaccgcgag gtagaagacc ccagcgacga tgaccggcgt cacgacaccc tcccaactga
      421 gtcggcccgc cgccaagacg cgggccggca cctcaggttt agccgggaag gcatgacagc
      481 gcgatcacag tgttacggcc ggtcgcgtca atttcgtgtc aggcgcgggc gccagtcgga
      541 ccgcggcagc ggtgatcgcc cccggcgcgc tcagttcccc gctgctccgg tctgggccgg
      601 ctgttcgcgc cggagcgcga ggccgagcca gaggtaccag gccgggttga ccagaaagcc
      661 gctgagcagc gcggggatga cgatgatggg attgctggag tccaagacga ccagctccag
      721 catgacggcc accacgaaga tggcggcatc gtcatggccc ccgttcggac acccctcccc
      781 gcgacgtgct actatttggc atccgcgaac ctggagcagg gtgacgtggg gcggagcgcg
      841 atcgcggcca gccggcttgc ggaatcggat gcgcatgggg gaaggttcag gcgcgatcga
      901 cggggaaggc acgccggatc tctccgtcgt cgtcccggca tacaacgagc gcggttgcat
      961 cgccgcctcg ctggcggcaa tgagcgccta tctcgacagc ctggcgctcg actacgagat
     1021 catcgtggtc tgcgatggga gccaggacgg cacggagcag atcgtcgccg cgttggcatg
     1081 ccagcattgc cggctgcggc tgatcagcta tccctgcaac cggggcaagg gcttcgccgt
     1141 gcgcaccggc atcacggcgg cgcggggccg cttcgtgatg ttcttcgacg ccgacctgac
     1201 cgtcccgatc accatcgtgc cgcagttcct ggcggcgctg cgggatggct acgacatcgc
     1261 catcgcctcc cgtcgtcacc cggcctcatc ggccctggcg ctgctgccgc gccggcgcca
     1321 ggtgatgggt caggtgttct cctggttcgt gcgccggctg gtgatcgacg acctgggcga
     1381 tacccagtgc ggcggcaagg cctatcgcgc cgaggtggct cgctgcctct tcggccgcca
     1441 gcgcatcgac catttcagct tcgacgccga ggtgctctac ctggccaggc gcctcaactg
     1501 ccggatcaag gaggtgccct tcgtgatcca gcacgcgccg agcggcagca gcatccaacc
     1561 agtgcgcgac tcgctcgtca tgctgcgcga tctgctgcgc atccgcctct atgcgctgcg
     1621 gggccgctac gagtgatgcc ggagagcccg caccaacccc gcccgctctc ccgcggtcct
     1681 atcggggaac cggcgacacg gcgccgggca atcatcttca acgccgacga cttcggattg
     1741 acggcggggg tgaacgccgg ggtgatcgag gcgcaccgtg cgggattggt gggctcggcc
     1801 tcgctcatgg tcaccacgcc ggccttcgac ggcgcgctgg agctggcgcg cgctcacccg
     1861 gcgctcgatc tgggcatcca cctggccctg accggtgtgc cgcccgcgct ggcgcatgac
     1921 gccgcttccg ggctggtggc cggtgaccgc tttcactcgc tgggcgtctt gctggcccgg
     1981 ctggccagcg ggcgcgtctc cgcgcccgcc ctgcgcgccg agctgcgggc gcagctcgaa
     2041 cgcgcgctgg cgagcgggct caccttcact catctcgata gccatcacca cgtgcacctc
     2101 ctcccgcaca tctcgaccgt ggtgggcgag ttggcccggg agtgcggcat tgccttcgtg
     2161 cggcgggtgc cggcgctgga agcgggcacg ccctgccgcc cggcacaggt gctcaagcgg
     2221 ctgatgctgc gcggcgcgga ctggcacgcg cagccagccc tgagcgggct gcgacacgcc
     2281 gatgcctttc gcgg
//
LOCUS       DASUBI010000786         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1246272, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000786 DASUBI010000000
VERSION     DASUBI010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246272"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VFU72_15395"
     CDS             complement(<1..1726)
                     /locus_tag="VFU72_15395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5424927.1"
                     /translation="MADEGDAPAAPAVRLALAGVENGRAAAIEPSEQQFRVLIERGND
                     LIAILDRDGAIRYASPSHQRMLGYQPQELVGVDALDLLHPDDRGLISEALESLLLRPD
                     GLTRVEYRLRHHDGSYRTFAAVGRNLLDDPVVRGIVVNSYDITERKNAEEALRRSEER
                     FRAIYEGAAIGIALLRRERGIVAANPALQAMLGYTEEELRTHSLRDRVHPDDLTIDQE
                     LFAELAAGRRHQYRIEKRYRHKDGRTIWGRLTMANVGEGYGLAEIIATLEDITEQKAL
                     ETRFIYDALHDPLTGLPNRALLLDRLEHALARTARQDHYLFAVLFIDLDRFKVINDRL
                     GHLIGDQALIAVAERIAGCLRPGDTVARLGGDEFVVLLDDIRCGDDAVTVATRIQAAL
                     RGALTIEGADVPISASVGISLNEDDFRHAERLLHDADTAMYRAKARGRGCCELYDHSM
                     SPDPAAPREAAALVAAIERQQLTLHYQPILALADARPRIVACEALLRGRHPERGLLPP
                     GEFMLAAEESGLMPVIDQFVLRGACRQAARWRARYGHRAPAISLNLSAKQLIQFDLAE
                     SVERALAAE"
     gene            complement(1902..3077)
                     /locus_tag="VFU72_15400"
     CDS             complement(1902..3077)
                     /locus_tag="VFU72_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEU5424928.1"
                     /translation="MARTARRLEGFGTSVFTEMSRLAVQHQAINLGQGFPDFPGPDLV
                     KHAAVEAIHANQNQYAPSHGTPRLRNAIAATFAASYGRSIDPEAEVTVTTGATEALQS
                     SMLAFVNPGDEVLFFEPFYDAYPAQVVFAGGVPRAVRLQPPDWHFDPDELARAITPRT
                     RVLLLNTPHNPTAKVFSRGELEVIARLAIEHDLLVLTDEVYDRIIFDGVAFQPIALLP
                     GMWERTVSINSTGKTFSMTGWKVGYCIAPAELGAAIRATHQFITFATPTPFQEAMAIA
                     LELADEQGYYGDLRGMYTARRDRLRAVLEGAGLPTLPCQGTYFLLADLTGLGFPDDVA
                     FCRYLTSEIGVAAIPPSAFYFDPASAPLLARFCFAKRPETIEAAAERLAGLPARAKH"
     gene            complement(3433..>4026)
                     /locus_tag="VFU72_15405"
     CDS             complement(3433..>4026)
                     /locus_tag="VFU72_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU5424929.1"
                     /translation="NWSFVRDDITRREVAFQIALALRDEVVDLEAAGIRIIQIDEPAL
                     REGLPLRREDWPIYLDWAVRAFRLASSGVQARTQIHSHMCYSEFNDIIEAIEALDADV
                     ISIENSRSGSELLRAFTNHRYARQIGPGVYDVHSPHVPSVAEMEALLEQASQVLEPDQ
                     IWVNPDCGLKTRGNAEVWPSLENMVAAARALRSAVPA"
BASE COUNT          590 a         1316 c         1496 g          624 t
ORIGIN      
        1 cctccgcggc cagggcgcgc tcgaccgact cggccagatc gaactggatg agctgtttag
       61 cggagaggtt gaggctgatg gcgggggccc ggtggccgta gcgggcgcgc cagcgagcgg
      121 cctggcggca ggcgccgcgc agcacgaact gatcgatcac gggcatcagt ccgctctcct
      181 cggcggccag catgaactcg cccggcggca ggagtccgcg ctcggggtgg cgcccgcgca
      241 gcagcgcctc gcaggcgacg atgcgcgggc gcgcgtccgc cagcgcgagg atcggctgat
      301 agtggagggt cagctgctgg cgctcgatcg ccgcgactag cgcggcggcc tcgcgcgggg
      361 ccgccgggtc tgggctcatg ctgtggtcgt agagctcgca gcagccgcgg ccccgtgcct
      421 tggcccgata catcgccgtg tcggcgtcgt ggagcaggcg ctcggcgtgg cgaaagtcgt
      481 cctcgttgag ggagatgccg acgctggccg agatgggcac atccgccccc tcgatcgtca
      541 gggcgccgcg cagcgcggcc tgaatccgtg tggccactgt caccgcgtcg tcgccgcagc
      601 ggatatcgtc gagcaagacc acgaactcat cgccgccgag ccgggccacg gtgtcgccgg
      661 ggcgcaggca gcccgcgata cgttcggcca ccgcgatcag agcctgatcg ccgatcagat
      721 ggcccagccg gtcgttgatg accttgaagc ggtcgaggtc gatgaagagg accgcgaaga
      781 ggtagtgatc ctgacgggcg gtgcgcgcca gggcgtgctc cagacggtcg agcaacagcg
      841 cccggttggg cagcccggtc aagggatcgt gaagggcgtc gtagatgaag cgcgtctcca
      901 gcgccttctg ctcggtgatg tcctccaacg tcgcgatgat ctcggccagc ccgtagccct
      961 caccgacgtt ggccatggtg agccggcccc agatggtgcg cccgtccttg tggcgatagc
     1021 gcttctcgat acggtactgg tggcgcctgc cggcggccag ctcggcgaac agttcctggt
     1081 cgatggtcag gtcgtcgggg tggacgcggt cgcgcaggct gtgggtgcgc agctcttctt
     1141 cggtgtagcc gagcatcgcc tgcagcgccg ggttggcggc cacgatgccg cgctcgcgcc
     1201 gcagcagcgc gatgccgatg gcggcgccct cgtagatggc tcgaaagcgc tcctcgctgc
     1261 ggcgcagcgc ttcttcggca ttcttgcgtt cggtgatgtc gtaggagttg acgacgatgc
     1321 cgcgcaccac cggatcgtcc agcaggttgc gcccgacggc ggcgaaggta cggtagctgc
     1381 cgtcgtggtg gcggaggcga tactcgaccc gtgtcagtcc atccggccgc agaagcaggc
     1441 tctccaatgc ctccgagatg agcccacgat cgtccgggtg gagcagatcg agcgcgtcga
     1501 cgcccaccag ctcttgcggc tgataaccga gcatccgttg atgcgacggg ctggcgtagc
     1561 ggatggcgcc gtcgcgatcg aggatggcga tcaggtcatt gccgcgctcg atcaggacgc
     1621 ggaactgctg ctcgctgggc tcgatcgcgg ccgcgcgccc gttctccaca cccgccagcg
     1681 cgagccgcac cgcgggggcg gcgggagcat ctccctcgtc ggccattctg cccactctca
     1741 acgagccgct ctgccggagc ggcgctcgac ctcttcgcgc cccggcagcg cggcgcacgc
     1801 gcgccgtggc gcgaccgtct gttgcactag atgtagtcga tggtggacgg cggacgcaaa
     1861 gaacgagatg ggcgagaagt cctgctttcg cgcaacgaga gtcagtgttt ggcgcgggcc
     1921 ggcagcccgg ccaggcgctc ggccgccgcc tcgatcgtct cgggccgctt ggcgaagcag
     1981 aagcgtgcca ggagcggggc gctggcggga tcgaagtaga aggcggaggg cgggatcgcg
     2041 gccacgccga tctcgctcgt caggtagcgg cagaaggcga cgtcgtcggg gaagccgagg
     2101 ccggtgaggt cggccagcag gaagtaggtg ccctggcagg ggagcgtcgg caggccggcc
     2161 ccctcgagca cggcgcgcag gcggtcgcgc cgggcggtgt acatgccgcg caggtcgccg
     2221 taatagcctt gctcgtccgc cagctccagc gcgatcgcca tcgcctcctg gaagggggta
     2281 ggcgtggcga aggtgatgaa ctggtgggtg gcgcggatgg cggcgcccaa ctcggctggg
     2341 gcgatgcagt agccgacctt ccagccggtc atgctgaagg tcttgccggt gctgttgatc
     2401 gacacggtgc gctcccacat gccgggcagc agcgcgatcg gctggaacgc gacgccgtcg
     2461 aagatgatgc ggtcgtagac ctcgtcggtc agcaccagca ggtcgtgctc gatcgcgagc
     2521 cgcgcgatga cctccagctc gccgcggctg aagaccttgg cggtggggtt gtgcggggta
     2581 ttcaggagca agacgcgtgt gcgcggcgtg atcgcccgcg ccagctcgtc ggggtcgaag
     2641 tgccagtcgg gcggctggag gcggacggcg cgcggcacgc ccccggcgaa gacgacctgg
     2701 gccgggtagg cgtcgtagaa cggctcgaag aagagcacct cgtcgccggg gttgacgaag
     2761 gccagcatgg aggactgcag cgcctcggtg gcgccggtcg tcaccgtgac ctcggcctcg
     2821 gggtcgatcg agcggccgta gctggcggcg aacgtggcgg cgatggcgtt gcgcaggcgc
     2881 ggcgtgccgt ggctgggcgc gtactggttc tggttggcgt ggatcgcctc gaccgcggcg
     2941 tgcttgacca ggtcggggcc ggggaagtcg gggaagccct gaccgaggtt gatcgcctgg
     3001 tgctgcacgg ccagccggct catctcggtg aagaccgagg tgccgaagcc ttcgagtcgg
     3061 cgagcggtac gggccacgtg gttcctcctt atgctgggcc ctcaccccag tctcggggag
     3121 ccctcacccc agccctctcc ggacggagcc gtgcgcggga gagggggact gccctccgac
     3181 gaggatgagt gccgggtggg gacggatcct tcgactgcgc tcaggatgac agggggaggg
     3241 atgaggcgta cgacgacgat cagcatcgtc cgcaaggccc catcccctac cgtgtcatct
     3301 cttcggcagg ctcagggcag gctctgtgcg cagtcgaagg atccgtccct cccttacgcc
     3361 caaccgacat cttcagccac ctcccctctc ccgcgcacgg ctccgtccgg agaggggccg
     3421 ggggtgaggg ccctacgccg gcaccgcgct gcgcaaggcg cgggcggcgg cgaccatgtt
     3481 ctccagcgag ggccagacct cggcgttgcc gcgcgtcttc aggccgcagt ccgggttgac
     3541 ccagatctga tccggctcca gcacctggct cgcttgctcc agcagcgcct ccatctccgc
     3601 cacgctcggc acgtgcggcg agtggacgtc gtagacgccg gggccgatct ggcgggcgta
     3661 gcggtggttg gtgaaggcac gcagcagctc cgagccgctg cggctattct cgatcgagat
     3721 gacgtcggcg tcgagcgcct cgatcgcctc gatgatgtcg ttgaactcgc tgtagcacat
     3781 gtggctgtgg atctgggtac gggcctggac ccccgaagac gccagccgga aggcgcgcac
     3841 cgcccagtcg aggtagatcg gccagtcctc gcgccgcagc ggcaacccct cgcgcagggc
     3901 cggctcgtcg atctggatga tgcggatgcc ggccgcctcc aggtccacca cctcgtcgcg
     3961 cagcgccagc gcgatctgga aggcgacctc gcgccgggtg atgtcgtcgc gcacgaacga
     4021 ccagtt
//
LOCUS       DASUBI010000787         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_75014, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000787 DASUBI010000000
VERSION     DASUBI010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75014"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFU72_15410"
     CDS             complement(<1..1142)
                     /locus_tag="VFU72_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5424930.1"
                     /translation="MTVAEMYVSDPRYPRCSDAEYARRHRLIREAMRERGLDCLIIYG
                     GYRNMYQQNFFWTSGYSDSFQGYIVFPLEGEPSLYNAVYPHLLPAKRQSVFPQTEWGG
                     PRMAETVANRLKELGLAQGKIGYVGIDTPRTVTIPHSHYETLTELLPQATFVDATPWF
                     MKIRFQKSPEEIEFIQRGAAITDGVMRDLVEAIHPGVWDYELFAVVYASALRQGGDSN
                     FALLASTSMDNPDMPYPYPVPSRRQIQKGDLVLNELCANYRGYSGQLIRPIAVGQPTA
                     ALSKLYDIAVELFYAVFGAIKPGNTTNDVIDAVRPIVARGDFNIQAPLVHGWDNLPQP
                     PLIGLPGGEREYPLDPHVFEEGQLIMIEPNPTSQDLSTGIFYGNLCV"
     gene            1473..2300
                     /locus_tag="VFU72_15415"
     CDS             1473..2300
                     /locus_tag="VFU72_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU5424931.1"
                     /translation="MNEQYHLESVERVVRVLNCFLEEPELRLTDLSARLGLHKTQVLR
                     IVSTLEAAGFLARDPATKQYRLGLQIFRLGMAMREQLDLRRVAHPVLERLGRETGETV
                     RLVVPDEDAAVCIDVIDSPQGLRVYAQIGARMPWNAGTSAKVVLAYLPDEDRERILAA
                     GGFKQLTPRTIVDPDELRADVLAIRRRGYHINIGDLQPDTRGVAAPIFNDRGELVASI
                     SISAPASRLQEPKSDHYIQTIVSAAREISTQLGFVPGAEPPAAAARSTQGAGVADRD"
BASE COUNT          386 a          780 c          895 g          407 t
ORIGIN      
        1 acgcagagat tgccgtagaa gatgccggtg ctgagatcct gactggtcgg gttgggctcg
       61 atcatgatca gttggccctc ctcgaagacg tgcggatcga gcgggtactc gcgctcgccg
      121 ccggggaggc cgatcaacgg cggctgcggc aggttgtccc agccatgcac cagcggcgcc
      181 tggatgttga agtcgccgcg cgccacgatc ggccgcacgg catcgatcac gtcgttggtg
      241 gtgttgccgg gcttgatggc gccgaagacg gcgtagaaga gctcgacggc gatgtcgtag
      301 agcttgctca gcgccgcggt cggctggccg accgcgatcg gccggatcaa ctggccggag
      361 tagccgcggt agttggcgca gagctcgttc agcaccaggt cgcccttttg gatctggcgg
      421 cggctgggga ccgggtaggg atagggcatg tccgggttgt ccatcgaggt cgaggcgagc
      481 agggcgaagt tgctgtcgcc gccctggcgc agggcgctgg cgtagacgac cgcgaacagc
      541 tcgtagtccc agacgccggg gtggatcgcc tcgacgagat cgcgcatcac tccgtcggtg
      601 atggccgcgc cgcgctggat gaactcgatc tcttcggggc tcttctgaaa gcggatcttc
      661 atgaaccagg gcgtggcatc gacgaaggtg gcctggggga gcagctcggt cagcgtctcg
      721 tagtggctgt gcgggatggt gacggtgcgc ggcgtgtcga tgccgacgta gccgatcttg
      781 ccctgcgcca gccccagctc cttgaggcga ttggcgaccg tctcggccat gcgcgggccg
      841 ccccactcgg tctgggggaa gaccgactgg cgcttggccg gcagaaggtg ggggtagacg
      901 gcgttgtaga gggacggctc gccctccagc gggaagacga tatacccctg gaaggagtcg
      961 ctgtagccgc tggtccagaa gaagttctgc tggtacatgt tgcggtagcc accgtagatg
     1021 atcaggcagt cgaggccgcg ctcccgcatc gcctcgcgga tcagccggtg gcggcgcgcg
     1081 tactccgcgt ccgagcagcg gggatagcgg ggatcgctca cgtacatctc ggcgacagtc
     1141 atacctgctc tctccctcac tcaacgactc tgagagcggg gccgcgccgc gggtcggtgc
     1201 gccgtcattt gccctgttgc tctcagagaa accatgtttc gccactgatg ctattctagg
     1261 ggggtgcgcg cgcggctgtc aaccgggccg cccgccggcg gcgcgagcga agcgccggag
     1321 cggtggtcgc gggcgagatt accggagttg cacttgcttc gaccgggttg cagaattgcg
     1381 cgggaaggtt caccgtgtgc ggaacggtgt ccggagaggc ggttgcgcct gcgtgcaact
     1441 gtccgctggc tgctggaatg gaacacgaga gcgtgaacga gcagtaccat ctcgagagcg
     1501 tcgagcgggt tgtcagggtc ttgaactgct tcctggagga gccggagctg cgcctgaccg
     1561 atctcagcgc gcggctcggc ctgcacaaga cccaggtgct gcgcatcgtc agcacgctgg
     1621 aggcggccgg tttcctggcg cgcgacccgg cgaccaagca gtaccggctg gggctgcaga
     1681 tcttccggct gggcatggcc atgcgcgagc agctcgatct gcgccgggtg gcccacccgg
     1741 tgctcgagcg cctggggcgc gagaccggcg agaccgtgcg gctggtcgtg ccggacgagg
     1801 acgccgccgt ctgcatcgac gtcatcgaca gtccgcaggg gctgcgggtc tacgcccaga
     1861 tcggggcgcg catgccctgg aacgccggca cctcggccaa ggtcgtgctc gcctacctgc
     1921 cggacgagga tcgcgagcgg atcctggcag ccggcggctt caaacagctc accccgcgca
     1981 cgatcgtcga cccggacgag ctgcgcgccg acgtgctggc gatccgccgg cgcggctacc
     2041 acatcaacat cggcgatctg cagcccgaca cgcgcggcgt ggccgcgccg atcttcaacg
     2101 accgcggcga gctggtcgcc tcgatcagta tctccgcgcc ggccagtcgc ctgcaggagc
     2161 cgaagtccga ccactacatc cagaccatcg tctcggcggc gcgggagatc tcgacccagc
     2221 tcggcttcgt ccccggcgcg gagccgcccg cggccgccgc gcgttcgacc cagggcgccg
     2281 gcgtggctga ccgcgactag agtctgcctg ccgacgacgg tgggtgccgc gtggggcacg
     2341 gatccttcga caagctcagg atgacagggc tagagaaggg ctgacgagga ggacgggccg
     2401 tggaagacgt taggtctagg acgatgacat atctcgtccg gcaggccgca tcccttcccc
     2461 tgtcatcc
//
LOCUS       DASUBI010000788         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_501608, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000788 DASUBI010000000
VERSION     DASUBI010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501608"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFU72_15420"
     CDS             complement(<1..308)
                     /locus_tag="VFU72_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424932.1"
                     /translation="MIAAALIAPISVILALLLPPAAQFFALPAIDPTIAYLLLSLGFL
                     GVLLETTHPGTTVPGVLGVVALAAGAVVLLTFDNAWWAIGVLLAAEMATLASAHWVRH
                     A"
     gene            466..1935
                     /locus_tag="VFU72_15425"
     CDS             466..1935
                     /locus_tag="VFU72_15425"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEU5424933.1"
                     /translation="MSTQRSIPRWLFSLLMLGLLMLPLLLLPGARADSFGAYAFQRLW
                     SRSDLPVVEGSASRAFVWGNQPFTGAVLEQYTEGTAQGVGGVRRVQYFDKGRMEITND
                     TADAGSTGYLTQGLLAREMITGRVQLGDKLFRDDGPAEINVAGDPDDLNAPTYATLKP
                     LMGYQPIPTGWIITQTVDRGGHLSDDPSLKPYGVTAAVLVPQTNHSVASVFWDYLRSS
                     GTISIGGHDAQGALFDDPLSISGYPLTEPYWTVVQLSGKPTRILVQAFERRVLTYTPD
                     NADGFKVESNNAGRDYYGWRYGAADGSAAPLSAGAVTFHPDADGNLIALGTLSNQART
                     AWQHVQVTVTLYDRFNQQVARQSSFLDFSVIASGEELPFRVWFDSAPVYDHYRVTASG
                     DVSAAQARGALVPASQEAQPLPGGGLVVNGAARNDASQPVANPLAVVALYDDSGDVVD
                     YAAVALSAATLGPGERAGYSAVLAAPPAAYTHYVVYFSS"
BASE COUNT          357 a          800 c          712 g          320 t
ORIGIN      
        1 gcgtggcgca cccaatgcgc cgaggccagg gtggccatct cggccgccag cagcacgccg
       61 atggcccacc aggcgttatc gaacgtcagc aacaccacgg cgccggccgc cagggcgacg
      121 acgccgagta ctcccggcac cgtggtgccc gggtgcgtcg tctccagcag cacaccgaga
      181 aagcccagcg agagcagcag gtaggcgatg gtcgggtcga tcgccggcag cgcgaagaac
      241 tgcgcggcgg gcgggagcag cagggccagg atcactgaga ttggagcgat cagagcggca
      301 gcaatcatga cgtatgggct ccttactcga cctaattata gcgccaacgg gctactgtta
      361 tccgccctgt ccatccttat cctcggagcc aggaagttcc agactggatg agcccaccca
      421 gccgaccgcg ccggtgggcg cgccgcacga cggggacgga tcacggtgag tacacaacgc
      481 tcgataccgc gctggctctt ctcgttgctg atgctgggtc tcctgatgct gccgctgctg
      541 ctcctgcctg gggcgcgggc cgactccttc ggcgcctacg ccttccagcg cctctggagc
      601 cgcagcgacc tcccggtcgt ggaaggcagc gcctcgcgcg cgttcgtctg gggcaaccaa
      661 cccttcaccg gtgccgtgct ggagcaatac accgagggta cggcacaggg cgtcggcggc
      721 gtgcggcgcg tgcagtactt cgacaagggc cgcatggaga tcaccaacga caccgccgac
      781 gccggcagca ccggctacct gacgcagggc ttgctggcgc gcgagatgat caccggtcgg
      841 gtccagctcg gcgataagct gttccgtgac gacgggccgg ccgagatcaa cgtcgctggc
      901 gatcccgacg acctcaacgc gccgacctac gccaccctca aaccgcttat gggctatcaa
      961 ccgattccga ctggctggat catcacccag accgtcgatc gcggcggcca cctcagcgac
     1021 gacccgtctc tgaaacccta tggcgtgacc gcggcggtgc tcgtgccaca gaccaaccac
     1081 agcgtcgcgt ccgtcttctg ggactacctg cgctcctcgg gaacgatctc gatcggcggg
     1141 cacgatgctc agggcgcgct cttcgacgac ccgctatcga tcagcggcta cccgctgacc
     1201 gagccctact ggacggtcgt gcagctctcg ggcaagccga cccgcatcct ggtgcaagcg
     1261 tttgagcgcc gcgttttgac ctacacgccg gacaacgccg acggcttcaa ggtcgaatcg
     1321 aacaatgccg ggcgcgacta ctacggctgg cgctacggcg ccgccgatgg ctccgccgcg
     1381 ccgctgagcg cgggcgcggt caccttccac ccggatgccg atggcaatct catcgcgctc
     1441 ggcacgctca gcaaccaggc gcgcaccgcc tggcagcacg tccaggtgac ggtgacgctc
     1501 tacgaccggt tcaaccagca ggtcgcccgc cagtcgagct tcctcgactt cagcgtgatc
     1561 gcctcgggcg aggaactccc attccgcgtg tggttcgata gcgcaccggt ctacgaccac
     1621 tatcgcgtca cagccagcgg cgacgtcagc gccgcgcagg cgcgcggcgc gctggtaccg
     1681 gccagtcagg aggcgcagcc gctgccgggc gggggcctgg tggtcaacgg cgcggcgcgc
     1741 aacgacgcca gccagccggt cgcgaacccg ctggcggtgg tggcgctcta cgatgactcc
     1801 ggcgacgtcg tcgactacgc cgcggtggcc ttgagcgcgg ccacgcttgg ccccggcgag
     1861 cgcgccggct acagcgcggt gttggccgcg ccgccggccg cgtataccca ctacgtcgtc
     1921 tacttctcgt cgtagtggtg cctggccggc ggccacgccg gtcaggccgt ctccacctgc
     1981 cgccacgctc atcacccacc ggccgtcaca atccacgagc ctgccgccaa agaaaccggc
     2041 cagcaccgct ggcggcgcga ctaccgcgcc ggtgagcccc agccgcgaga tcacggaggc
     2101 tcccaagggc gcgagggtcg gtccgtcatc ccgccgcgcc gccgtcgtgc ccacggtttg
     2161 ccgcgccacc ccggcggcgt cttaggccg
//
LOCUS       DASUBI010000789         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_282693, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000789 DASUBI010000000
VERSION     DASUBI010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282693"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFU72_15430"
     CDS             <1..442
                     /locus_tag="VFU72_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5424934.1"
                     /translation="NMSLSTGKPQFCLALHELADRAYFRRCVIVASAHNMPVRSYPWP
                     FASVISVASHDEHDPSIYYYNPSPPVDFFARGVGVEVAWPGGAKIRSTGNSFATPHIT
                     GICARILGKHPMLTPFQLKSVLYLAASNVFEPPTAPQGGEHASR"
     gene            429..1109
                     /locus_tag="VFU72_15435"
     CDS             429..1109
                     /locus_tag="VFU72_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5424935.1"
                     /translation="MPAANAPTPRWSDDRAGITERSLLQSIVEVARSVFGAAAASVFL
                     LDEETGDLVFEAVAGEGEDQLVGTRFPSGTGLAGWVVMSGQALLVDDVTQNPQFAKDA
                     AESTGYVPRTIMAAPLTRNGDCIGVLEVLDRGSRPRGDLSDVDLIGMLATEVALGLEL
                     VVRLRWLAGEQESATAGSDGDLGRLRRIAARLPAASEPIASTVSRLLATADDLLADDS
                     ASVSDVSR"
     gene            1220..2209
                     /locus_tag="VFU72_15440"
     CDS             1220..2209
                     /locus_tag="VFU72_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424936.1"
                     /translation="MLLVSRAGDSELGHVRRQLEAVGISVARLDAEAAAGLTVDPHRR
                     MVSIGGRWISPTVTWVRHFSHRAMPQGRSTLRRAYAADSWQALADQLDVLSAEMITYR
                     EPGLLAQLETARGCGVAVPATVVTTNPARAAELLPGSKLVVKALHRHFVEARPGLLHG
                     IFPEVIDREDLRRIPAGPPVVVQEHIDHDAEIRVYHVHGQIAAAFAITKSRAAEPWQH
                     PELVTARQIDPPDAVAAAASAVAEALSIDYGAMDFLISGGVPVFLEVNLAGDWRWLEV
                     KVRKAPVTAAVAAMLRVKHEQIAGAARPTKISPVTFLTRGVPPTGVDKDEPTL"
BASE COUNT          368 a          779 c          729 g          363 t
ORIGIN      
        1 caacatgagc ctgtcaacag gaaagccgca attctgcctt gccctgcacg aactcgccga
       61 tcgcgcctac ttccgccgct gcgtgatcgt tgcctcggca cacaatatgc cggtccgcag
      121 ctatccctgg ccgttcgcct ccgtcatctc agtcgccagc catgacgagc acgacccgtc
      181 tatctactac tacaacccgt cgccaccggt tgacttcttc gcccgcggcg tgggggtcga
      241 agttgcctgg ccgggcggcg cgaagattcg cagcactgga aatagcttcg cgacgccgca
      301 catcaccggt atctgcgcgc gtattctcgg caagcacccg atgctgacgc cgtttcaatt
      361 gaaatcggtg ctctacctgg ctgccagcaa tgtcttcgag ccgccaacgg caccgcaggg
      421 aggcgaacat gccagccgct aacgcaccga cgcctcgctg gtctgacgac cgcgccggca
      481 tcaccgagcg cagcctgctc cagtcgatcg tcgaggtggc aaggagcgtt ttcggcgccg
      541 cggccgcctc ggtgttcctg ctcgacgagg aaaccggcga ccttgtgttc gaggcggtgg
      601 ctggcgaagg ggaagaccag ctcgtcggca ccagatttcc gagcggcacc ggcctagccg
      661 gatgggtggt gatgagcggc caggcgctgc tcgtcgacga cgtcacccag aacccgcagt
      721 tcgccaagga cgccgccgag tcaaccggct atgtaccgcg caccatcatg gccgctccgc
      781 tcaccaggaa cggcgactgc atcggagtgc tcgaggtact cgatcgcggt agcaggccgc
      841 gcggtgacct ttccgacgtc gatctcatcg gcatgctggc cacggaagtc gcgctcggcc
      901 tcgagcttgt cgtcaggctg cgctggctgg ccggcgagca ggagtcagcg acggcggggt
      961 ccgacggcga tcttggcagg ctgcgccgga tcgccgcccg gctgcctgcc gcttctgagc
     1021 cgatcgcgtc gacggtcagc cgactgctcg ccaccgccga tgacttgctg gccgacgaca
     1081 gcgcctccgt gtcggatgtg tcgcgctgac ggcacccttc agctggcagc tcgccgggcg
     1141 gggctacgtg ctcaacctcc gggccgagca ctcacgatcg ccggccaaac gcacgagcgc
     1201 tggccagtca ggtccagccg tgctgctggt cagccgggcc ggtgactccg aactcggcca
     1261 cgtcaggcgg cagcttgagg cggtcggcat atcggtcgcc aggctggatg ccgaggccgc
     1321 ggccggcctg acagtcgatc cgcaccgccg catggtaagt atcggtggcc ggtggatcag
     1381 cccgaccgtc acgtgggtca ggcacttttc ccaccgcgcg atgccgcaag gccgcagcac
     1441 gctgcggcga gcctacgcgg ccgactcctg gcaggcgctc gccgaccagc tcgatgtgct
     1501 gtccgccgag atgatcacct accgggaacc tggcctgctc gctcagcttg agacggcgcg
     1561 cggctgcggc gtcgcggtgc ccgcgaccgt cgttaccacc aatccggcgc gggcggccga
     1621 actgctgcct ggctccaagc tcgtggtcaa ggcgctgcac agacacttcg tcgaggcgag
     1681 gccaggactg ctgcacggca tctttcccga ggtcatcgac cgcgaggacc ttcgccgcat
     1741 cccggccggc ccgccggtcg tcgtgcaaga acacatcgac cacgacgccg agatccgcgt
     1801 gtatcacgtg cacggtcaga tcgcagcggc gttcgcgatc acgaagtcgc gtgccgctga
     1861 gccttggcag catcccgagc tggtgacggc caggcagatc gaccctcctg atgccgtcgc
     1921 cgcggcggcc tcggcagttg cggaggccct gtcgatcgac tacggcgcga tggactttct
     1981 catcagcggc ggcgtccctg tcttcctgga ggtcaacctc gccggggact ggcgatggct
     2041 ggaggtcaaa gtgcggaagg cgccggtgac cgcggcggtc gcggcgatgc tgcgcgtcaa
     2101 gcacgagcag atcgccggcg cggcgaggcc gaccaaaata agtcctgtca cattcctgac
     2161 ccgcggcgtc ccgccgaccg gtgttgacaa ggacgaaccc acgctatagg aacaaacaag
     2221 gcagaccgtc gcgtcagtc
//
LOCUS       DASUBI010000790         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_760470, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000790 DASUBI010000000
VERSION     DASUBI010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760470"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFU72_15445"
     CDS             <1..625
                     /locus_tag="VFU72_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEU5424937.1"
                     /translation="VSDHGAQAMRGAVCINEWLRQEGYLVLCEEPQGSVPFSQAQVDW
                     ERTRVWGEGGYYCRLCLNVAGREPQGIVPPEEFEALRDELTAKLVALRGPDGCPLGNR
                     VYRPGDLWAAERGIPPDLIVYFGDLAWRSNGSLGHGQLFTFENDTGPDDANHAKDGLC
                     ILAGPGVTPGELHGLSLYDIAPTVLRIFGLPIPEEMRGRVLPALDHR"
     gene            693..1136
                     /locus_tag="VFU72_15450"
     CDS             693..1136
                     /locus_tag="VFU72_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424938.1"
                     /translation="MLARSTVFQGRLPRAAAYGLVAGAATLLLIGIPTAAIPNRFFER
                     MVPATPANYVVLALTVLLAAALGASYALPAACRLQEGKLTAGGMLSFLAVGCPVCNKI
                     VVLLVGASGAVSYFEPLQPLLGVLSLVLLGVALRTRLRAVRAAGA"
     gene            1216..3435
                     /locus_tag="VFU72_15455"
     CDS             1216..3435
                     /locus_tag="VFU72_15455"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU5424939.1"
                     /translation="MAEIEQQAKHEQRVEQQLENGTHRILQQGHPAVTVRVPDSMALK
                     QGNMFLTCTQSGDILRGTDLGLYFHDMRYLSEQSLQIEEQQPVYLFAGLVSGGGGATY
                     QLTNPDIHDKDGVALIRKDSIGIRRDKMLGNEYTETVTVENYRERRLDFSLRLCYDAD
                     FEDMFVVRGAQPGKRGTLHPPAWDGNALSQRYDGADGHMRQTTIHFIPAPDHIEGTHC
                     TYHISLAPRETWHFQVVAELLDVNDGDLELTPTTFAAVNKEVLEHAEHGALNGGMSIV
                     SDNPLFNNILTRSLLDLHVLAMRQFGQPFYAAGIPWFVALFGRDSLVTTIQMAAFQPR
                     MAENTLRVLASHQGTKVDHYRDEEPGKILHELRVGEMANLGEVPQTPYYGTVDATILF
                     LIVMGIYSNWVGNLELFGELRDNVERALSWIDKYGDSDGDGFIDYKTHSSKGLRNQGW
                     KDSGNAIVMHDGTLAEPPIALPEVQGYAYLAWVMMADLYRRSGNAERADRLLRQAGDL
                     RQRFNEHFWVSPIQYFAFCRQADGRYSHSIASNAAQALWTGIIADEHVADVVARVMQP
                     DMFTGWGIRTLSANDRSYNPLDYQVGSVWPHDTALIAGGMYGSGHAEEAGRVFSALLH
                     AAFRFEHFRLPETFCGYDRSYADQPVRYPVACSPQAWAAGAVPYLIQTALGLQPDGFN
                     RRLRVVRPSLPDRLQWVTVRNVTLAECSVDLHFERVNDATLATVLRKEGDVTIAVEY"
     gene            3521..3593
                     /locus_tag="VFU72_15460"
     tRNA            3521..3593
                     /locus_tag="VFU72_15460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Lys,seq:ctt)
     gene            3638..4210
                     /locus_tag="VFU72_15465"
     CDS             3638..4210
                     /locus_tag="VFU72_15465"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU5424940.1"
                     /translation="MTTTAPSPVLTRYRFSVDDFQRMGEAGIFGEDDRVELLDGEIIT
                     MNPIGGPHMTSVNRLTRRLVLALGEDAIVSIQNPIVLGRHDEPQPDVVVLRSEADQHA
                     GKPVPDDVLLLIEVADSTLAYDRDVKLPRYAQSGIPETWIVDLAGRLITRYTEPTAEG
                     YRVATTFRPGQAIASASLPALQLAVADILP"
BASE COUNT          931 a         1860 c         1859 g          902 t
ORIGIN      
        1 cgtctccgac cacggcgcgc aggccatgcg cggcgcggtc tgcatcaatg aatggctgcg
       61 gcaggagggc tacctggtgt tgtgcgagga gccgcagggc agcgtgccct tcagccaggc
      121 gcaggtcgac tgggagcgca cccgcgtctg gggcgagggg ggctactact gccggctctg
      181 cctgaatgta gccgggcgcg agccacaggg gatcgtaccg cccgaggaat tcgaggcgct
      241 gcgcgacgag ctgaccgcca agctcgtggc gctgcgcggg ccggacggct gcccgctcgg
      301 caaccgggtc taccgcccgg gcgatctctg ggcggccgag cgcggcatcc cgcccgactt
      361 gatcgtctac ttcggggatc tggcctggcg cagcaacggc agcctgggcc acggtcagct
      421 cttcaccttt gagaacgaca ccggtcccga tgacgccaac cacgccaaag atgggctgtg
      481 catcctggcc gggccagggg tcacacccgg cgagctgcac ggcctctcgc tctacgacat
      541 cgccccgacg gtgctgcgca tcttcggcct gccgatcccc gaggagatgc gcggccgcgt
      601 gctgccggcg ctcgatcatc gttgatggag tggcgccggc tgggcgtata ctgcgtgaag
      661 tgtgcagtgt gccgacgtcg atggaggagc ccatgctcgc gcgttccacg gtcttccagg
      721 ggcggctgcc ccgcgcggct gcctacggcc tcgtcgccgg cgccgcgaca ctgctgctga
      781 tcggcatccc gaccgcggcc atccccaacc gcttcttcga gcgcatggtg ccggcgactc
      841 ccgccaacta cgtggtgctg gcgctcacgg tgctgctggc ggccgcgctg ggcgccagct
      901 acgcgctgcc cgccgcctgc aggctgcagg agggcaagct gaccgccggc ggcatgctct
      961 cgttcctggc ggtcggctgc ccggtctgca acaagatcgt ggtgctgctg gtcggcgcca
     1021 gcggcgcggt gagctacttc gagccgctgc agccgctgct gggcgttctc tcattggtgc
     1081 tgctcggggt ggcgctgcgg acgcggctgc gtgcggtgcg ggccgccggc gcgtaacccc
     1141 tgtcggttct ggcattctcc ctgctaaacc gggggccagg cgcgggcatc gtgcccgcac
     1201 gcagggaggt tgccggtggc cgagatcgag cagcaggcca agcacgagca gcgagtagag
     1261 cagcagctag agaacgggac gcaccgcatc ctgcagcagg ggcatccggc ggtcaccgtg
     1321 cgtgtgcccg actcgatggc gctcaagcag gggaatatgt tcctgacctg cacgcagagc
     1381 ggggacatcc tgcgcggcac cgacctcggc ctctacttcc acgacatgcg ctacctgagc
     1441 gagcagagcc tccagatcga ggagcagcag ccggtctatc tcttcgccgg gctggtctcc
     1501 ggcggcgggg gcgccaccta tcaactgacc aaccccgaca tccacgataa agacggcgtg
     1561 gcgctcatcc gcaaagactc gatcggcatc cggcgcgaca agatgctcgg caacgagtac
     1621 accgagaccg ttaccgtcga gaactaccgc gagcgccggc tcgacttctc gctgcgcctc
     1681 tgctacgacg ctgacttcga ggacatgttc gtggtgcgcg gcgcccagcc gggcaagcgc
     1741 ggcaccctgc acccgccggc ctgggacggc aacgcgctga gccagcgcta cgacggtgcc
     1801 gacgggcaca tgcgccagac gacgatccac ttcatcccgg cgcccgacca catcgagggc
     1861 acccactgca cctaccatat ctcgctggcg ccgcgcgaga cctggcactt ccaggtggtg
     1921 gcggagctgc tggacgtcaa cgacggcgac ctggaattga cgccgaccac cttcgccgcg
     1981 gtgaacaaag aggtgctgga gcacgccgag cacggggcgc tcaacggtgg catgagcatc
     2041 gtgagcgaca acccgctctt caataatatc ctgacgcgct cgctgctcga cctgcacgtc
     2101 ctggcgatgc gccagttcgg ccaacccttc tacgccgccg gtatcccctg gttcgtggcc
     2161 ctcttcggac gcgacagcct ggtgacgacg attcagatgg cggccttcca gccgcgcatg
     2221 gccgagaaca ccctgcgcgt gctggcctcg caccagggca ccaaagtaga ccactatcgc
     2281 gacgaagagc cgggcaagat cctgcacgag ctgcgggtag gcgagatggc caatctgggg
     2341 gaggtgccgc agactcccta ctacggcacg gtcgacgcga ccatcctctt cctgatcgtg
     2401 atgggcatct actccaattg ggtcggcaac ctggagttgt tcggcgagct gcgcgacaac
     2461 gtcgagcgag ccctgagctg gatcgacaag tacggcgact ccgacggcga cggcttcatc
     2521 gactacaaga cgcattcgtc aaaggggctg cgcaaccagg gctggaagga ctcgggcaac
     2581 gccatcgtga tgcacgacgg cacgctggcc gagccgccga tcgcgctgcc cgaggtgcag
     2641 ggctacgcct atctcgcctg ggtgatgatg gccgacctct atcgccgcag cggcaatgcc
     2701 gagcgagccg accgcctgct gcgccaggcg ggggacctgc gccagcgctt caacgagcac
     2761 ttctgggtct cgccgatcca atacttcgcc ttctgccgtc aggccgacgg ccgctactcg
     2821 cacagtatcg cctccaacgc ggcgcaggcg ctctggacgg ggatcatcgc cgacgagcac
     2881 gtggcggacg tggtcgcgcg ggtgatgcag ccggacatgt tcaccggctg ggggattcgc
     2941 accctgtcgg ccaacgaccg ctcctacaac ccgctcgatt accaggtcgg cagcgtctgg
     3001 ccgcacgaca cggcgctgat cgccggaggg atgtacggca gcgggcacgc cgaggaggcg
     3061 ggacgtgtct tcagcgcgct gctgcacgcc gcctttcgct tcgagcactt ccgtctgccg
     3121 gagaccttct gcggctacga ccgcagctac gccgaccagc cggtgcgcta cccggtggcc
     3181 tgcagtccgc aggcctgggc ggcgggcgcg gtgccctatc tgattcagac ggcgctgggg
     3241 ctacagccgg acggcttcaa ccggcgcctg cgggtggtgc ggcccagcct gcccgaccga
     3301 ctgcagtggg tcacggtgcg caacgtgacc ctggccgagt gcagcgtcga cctgcatttc
     3361 gagcgggtga acgacgccac gctggcgacg gtgctgcgca aagaggggga cgtcaccatc
     3421 gcggtggagt actgagcgac ggggagcgcg tgagcggccg tttgcccgaa gtcgggtgca
     3481 ttgagtatac ttttacggtc tcgggcaggc ccgagcgact gggccgctag ctcaattggc
     3541 agagcagctg actcttaatc agcgggttcg gggttcgagt ccctggcggc tcacttaggt
     3601 tcaacgatcc aacaagccac gattgggagg tgccggcatg acgaccacag caccctcccc
     3661 ggtgctcacg cgctaccgct tcagcgtgga tgacttccag cgcatgggcg aggcgggcat
     3721 cttcggggag gacgaccggg ttgagctgct cgacggcgag atcatcacga tgaatcccat
     3781 cggcggcccg cacatgacct ccgtcaaccg gcttaccagg cggttggttc tggcgctcgg
     3841 cgaggatgcc atcgtcagca tccagaaccc catcgtgctc ggccggcacg acgagcctca
     3901 gccggatgtg gtcgtgctcc ggtcagaggc cgatcagcac gcgggcaaac ccgtccccga
     3961 cgacgtgctc ctcctcatcg aggtcgccga cagcacgctg gcctacgacc gcgacgtgaa
     4021 gctgccgcgc tacgcccagt ccggcatccc cgagacctgg atcgtcgatc tcgccggccg
     4081 tctgatcacg cgctacaccg agccgacagc cgagggctac cgcgtcgcga ccaccttccg
     4141 cccgggccag gccatcgcct ccgcgtcctt gcccgcgctc cagctcgcgg tggccgacat
     4201 cctcccgtag cctcctgggc acagcctctt ggcagggcat ctgactctta attaagtggg
     4261 tcctgggttc gagtccctgg cggctcacca gcagccggaa tcctcgtacg aagacggggt
     4321 tccggctttt gtactgcctc gtgcggtgtc ccagctgacc gtgcagcagg tgcccgggca
     4381 tacgaccccg gatcccgaag gaaccgagag gaatcgcggc accaaggcgg tgcgttcgag
     4441 cgggaacgcc tgcgcgccag gaggccaagc gtgctggcgc ggatcgcggg gacgacggtg
     4501 attgccgcat cacctccacc ctggcgaaac attccgcgta gcacccctgt ggcactcctg
     4561 tcgaggccgc gaacggctga cattgcgcct acctgttgcg atatgcagcg accacgcctg
     4621 agggggccgt cgcgacacca aacgacgtcc taacctcgag cgaagggtcg atcgaggttc
     4681 gaggaggatc cgaataatga cggtcgggcg tcgcgttgag atcgcggaat tgagatcaga
     4741 tcttgaaaac cgccatctgt gttggtgctc gggtgaggcc aagttcgagt tcgaggcgcc
     4801 cgttgacacg gcgtttgacc ataccagtcg taggggaaca ggggttcact cgagaacgga
     4861 tactgcagca ttccgctcaa tggataagga gggaggcggg cggggaagcc cgcctccaga
     4921 agagaaaggg ggggagggaa ggggacgacc tggagcgcag ggggaatcgg gtgcggtcca
     4981 gacgatccgt cgagcatgcg attgatacgc tgctgccatc gcttcccgat agcagcgcct
     5041 gcgcgtcggt ctctcgcgct ccctgcagga cgcatgcgat cgctcgctcc gctgaactca
     5101 ggaggcagcc gccgcggctg ccttggctcc ttctgggtgt cgttgatcca gcttcagcgg
     5161 gccgcgtgca cggccccgag tcgatcaggg ggctcaggct cccgcaccca aataggactc
     5221 tacccatgac ccatgaaata gccggggtcg tctcatgaac atcgcgtgaa cagccgggat
     5281 cattttggcg aggagggttg gtcgctcgag gtcggtgcat cgagaccgcg cgaccggccg
     5341 ctacaccggc ttggccacga cactcagccg gtcgtcatct catggcatcc ggtgcggcgt
     5401 gacctgccac cgtatcgagc atgcgcgacc agctggctga tcccctcata ggttgtccat
     5461 ggtggcgcat ggtcaggctc tgcacgcgtc ctatcccgag cccgaacggc actgcgaacc
     5521 atgctgctct ctaacgtggt gcgagagtgc cg
//
LOCUS       DASUBI010000791         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_933950, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000791 DASUBI010000000
VERSION     DASUBI010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933950"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFU72_15470"
     CDS             <1..638
                     /locus_tag="VFU72_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5424941.1"
                     /translation="TGGLLDAVGIIRRGSGRERVPVHAHPDLFKPSFSERNGKLSFIG
                     LPHTRPALEYAGADFRLETGWQEIAPGMCMTGEVPRRHAFELGDQNMQHCDEAGRIVV
                     DPVRDDQTVVFDLPAGLFVVLGCAHAGLINILDYIQAQTGKTHFHTVMGGTHLGGVGE
                     EQVEQTLAALRDYDIAHLGVSHCTGQKVGARLAHEYGDRFFFCSVGKVVEV"
     gene            complement(724..2064)
                     /locus_tag="VFU72_15475"
     CDS             complement(724..2064)
                     /locus_tag="VFU72_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424942.1"
                     /translation="MTQADRQAASKGTGGPGSDVSGEPGGVLKAGERAATTVADLVGL
                     TEDRGVRGIVVGGSLTGVPTLHLAPGQSLRGDSDRGEIVFAPGSDGLRLSADNRVSAL
                     RLQASPERRAIFNDTSVASLGQIQLHDVTTIGRVQILARDAVRGGHVEVRGLDIVAAD
                     ARQETERPHGYGVFVLQGAFTLWNRQPDEGVIVTADLLGLSAGRAARPVLGSGVFVSG
                     AGDAGGRVSVRRLETGPIYSDGRIAPGTPDQITGGVFTVYGTDVGTVRNRGPVVTYGQ
                     NDMALDNWGTVDQWIAEAPIASYGPSGIGFVNFGTVHELRAEAPIETFGQGARGFNVY
                     TGTVRHAEFDRVVTHGDGAVGIQISQPIGRLVVRRGIETFGATGESLVKGVLVSLSAI
                     GLSIKPGGSALEITIQGGLTTHGRGIVPIEQHGAIESIQISGGFAALGGGFDQV"
     gene            complement(2181..3533)
                     /locus_tag="VFU72_15480"
     CDS             complement(2181..3533)
                     /locus_tag="VFU72_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5424943.1"
                     /translation="MTTTKTWNKADLVQKDKAYLLHPVTNLHQIKEYGPLIITHGDGV
                     YIWDVDGKRYIDAFAGLWNVNIGHGNQELGAAMKEQADEIAFVPTFYGLAAPPTIELA
                     AKLADMFPDPINHFNFTSGGAESNESAIKFARYYWYLKGKPDKVKILSRMMGYHGIAM
                     GALSATGIPAYWTGFGPRPEGFLHLTAPYHYRNGEGLSEDQFVDKLVEELEQTIQREG
                     ADTIAALIGEPVQGAGGVVVPPDKYWPRMAEVLRKHNILLIFDEVITGFGRTGSMFGM
                     QQYGVTPDIVAFAKGITSGYVPLGGVGVTDEVFDVMSEPDQLLYHGFTYSGHPVCCAV
                     ALKNIAIIENQHLADNAREVGGYMLDELKKLLDRPYVGNVRGKGLMMLVEVVKDKQTK
                     EKFGAELGGKLQAATRKHGIIVRASSEVIAMSPPLIITKSQADDVVQALGAALQEVLG
                     "
     gene            3692..4336
                     /locus_tag="VFU72_15485"
     CDS             3692..4336
                     /locus_tag="VFU72_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424944.1"
                     /translation="MTTSGQGGEEYFIDSPALRGFVGAVRAAIAEADSPGPLVERLRP
                     PFARLLADQAWLPAEFAQPDETSGMGGGIGQYLLYRSESPVFTIFSLVVPSGAMTPVH
                     DHLAWGLIGLYRGEQREEMYALMAGNPDAGEAKIELLEERRVGPGDFYAILPPDNDIH
                     RVTTTSAGPSISIHLLGNDTGCVLRHTYDQAAETSHSFRSGYSNAPCEADGEAG"
BASE COUNT          677 a         1586 c         1481 g          737 t
ORIGIN      
        1 acaccggcgg gctgctcgac gcggtcggga tcatccgccg cggatcgggc cgcgagcgcg
       61 tgccggtcca cgcccacccc gatctcttca agccgagctt ctccgagcgc aatgggaagc
      121 tcagcttcat cggtctaccc cacacgcggc cggcgctgga gtacgcgggc gccgacttcc
      181 gcctggagac cggctggcag gagatcgcgc cggggatgtg catgaccggc gaggtccccc
      241 ggcgccacgc cttcgagctg ggcgaccaga atatgcagca ctgcgatgag gcgggccgga
      301 tcgtggtaga cccggtgcgc gacgaccaga cggtcgtctt cgacctgccc gccgggctct
      361 tcgtggtgct cggctgcgcc cacgccggcc tgatcaacat cctcgactac atccaggcgc
      421 agaccggcaa gacacacttt cacaccgtca tgggcgggac gcacctgggc ggggtggggg
      481 aagagcaggt ggagcagacg ctcgccgcgc tgcgcgacta cgacatcgcc cacctgggcg
      541 tgtcgcactg caccgggcag aaagtgggcg cgcgcttggc ccacgagtac ggcgaccgtt
      601 tcttcttctg cagcgtcggc aaagtcgtcg aggtgtagcg cagcggggcg gtcaggcgac
      661 caccccgcga aggcgcgact gatgatgcgc ccgagtacgg cacgaaggcg ccttccggtc
      721 gcgttacacc tggtcaaacc ctccacccag ggccgcgaat ccaccgctaa tctggatgga
      781 ttcgatcgct ccgtgttgct cgatcggcac gatgccgcga ccgtgggttg tcagtccccc
      841 ttggatcgtg atctccaacg ccgagccacc cggcttgata ctcagcccaa tagcagagag
      901 actgaccagc acccccttca ccagcgactc tccggtcgcc ccgaaggtct cgatgcctct
      961 ccgcaccacc agccggccga ttggctggct gatctggatg cccaccgcgc catcgccatg
     1021 ggtgaccacc cggtcgaact cggcgtgccg caccgtgccg gtgtacacat tgaagccgcg
     1081 ggccccctgg ccaaaggtct cgatcggtgc ctcagcccgc agttcgtgca cggttccgaa
     1141 gttgacgaac ccgatgccgc tggggccgta ggaggcgatc ggcgcttcgg cgatccattg
     1201 atcgacggtg ccccagttgt cgagcgccat gtcgttttgg ccataggtca cgaccgggcc
     1261 acggttgcgc actgtgccga cgtccgtccc gtagacggtg aagaccccgc cggtgatctg
     1321 gtccggagtc cccggcgcga tccggccatc actgtagatc ggtccggttt ccaaccggcg
     1381 gactgagacc cggccgcccg catcccccgc gccgctcacg aacaccccgc tgcccaatac
     1441 gggacgcgcg gcgcgccccg ccgacagccc aagcaggtcg gccgtcacga tcacgccctc
     1501 gtccggctgc ctgttccaga gggtgaacgc tccctgcaag acaaagactc cgtagccgtg
     1561 cgggcgctcc gtttcctggc gggcgtccgc cgccacgatg tcgaggccgc gcacctcgac
     1621 gtggccgccc cgcaccgcat cacgggccag gatctggacc cggccaatcg tcgtcacgtc
     1681 gtgtagttgg atctgcccca gactcgcgac cgacgtgtcg ttgaagatcg ctcgccgctc
     1741 gggcgacgcc tgtaggcgca gcgcgctgac gcggttatcc gcggacaacc gcagcccgtc
     1801 gctcccaggc gcaaacacga tctcgccgcg atcgctgtcg ccgcgcagcg actggccagg
     1861 agcgaggtga agtgtgggca cgccggtcag cgagccgccg acgacaatcc cacgcacgcc
     1921 ccggtcttcg gtgagcccca ccagatccgc gacggtggtg gcggcccgtt cccccgcttt
     1981 cagtactccg cctggttccc cggagacgtc ggatcctggt ccaccggtgc ctttgctcgc
     2041 tgcctgccgg tctgcctgcg tcatgatcgc tctcctgcag tgctacggat ccggatgcgc
     2101 cggaaaccac ggcggcagat catgcggcgg cccgcgccgg gtcgcaccca tgcgcacggc
     2161 ccggcgcggc gctccttacc ctagccgagg acctcctgca gcgcggcgcc gagcgcctgg
     2221 acgacgtcgt cggcctggct cttggtgatg atcagcggcg gcgacatcgc gatcacctcg
     2281 ctgctggcgc gcacgatgat gccgtgcttg cgggtggccg cctgcagctt gccgcccagc
     2341 tcggccccga acttctcctt ggtctgcttg tccttgacca cctcgaccag catcatcagc
     2401 cccttgccgc gcacgttgcc gacatacggc cggtcgagca gcttcttgag ttcatcgagc
     2461 atgtagccgc cgacctcgcg cgcgttgtcg gccaggtgct ggttctcgat gatggcgatg
     2521 ttcttcagcg ccacggcgca gcagaccggg tgacccgagt aggtgaagcc gtggtagagg
     2581 agctgatcgg gctcggacat gacatcgaag acctcgtcgg tgacgccgac gccgcccagc
     2641 ggcacgtagc ccgaggtgat ccccttggcg aaggcgacga tgtcgggcgt gacgccgtac
     2701 tgctgcatgc cgaacatgct gccggtgcgc ccgaagccgg tgatgacctc gtcgaagatc
     2761 aagaggatat tgtgcttgcg cagcacctcg gccatgcgcg gccagtactt gtccggcggc
     2821 acgaccacgc cgccggcgcc ctgcaccggc tcgccgatca gcgccgcgat ggtgtcggcg
     2881 ccctcgcgct ggatcgtctg ctccagctcc tcgaccagct tgtcgacgaa ctgatcctcg
     2941 ctcagcccct cgccgttgcg gtagtggtag ggggcggtca gatgcaggaa gccctcgggc
     3001 cgcgggccga agccggtcca gtaggccggg atgccggtgg ccgagagcgc gcccatggcg
     3061 atgccgtggt agcccatcat gcgcgagagg atcttgacct tgtccggctt gcccttgaga
     3121 taccagtagt agcgcgcgaa cttgatggcc gactcgttgg actcggcgcc gccgctggtg
     3181 aagttgaagt ggttgatcgg gtcggggaac atgtcggcca gtttggccgc cagctcgatg
     3241 gtgggcgggg cagccaggcc gtagaaggtg ggcacgaagg cgatctcgtc cgcctgctcc
     3301 ttcatggccg cgcccagctc ctggttgccg tggccgatgt tgacgttcca gagcccggcg
     3361 aaggcgtcga tgtagcgctt gccgtcgacg tcccagatgt agacgccgtc gccgtgggtg
     3421 atgatcagcg ggccgtactc cttgatctga tgcaggttgg tgacggggtg cagcaggtac
     3481 gccttgtcct tctgcaccag gtccgctttg ttccaggtct tggtcgtcgt catctgggca
     3541 atccctccgc ttgaccgtgg ccgatccgtc catcacgcac actctacggc agcgcgcccg
     3601 cctcgtccac gccgctgctg accctctgac ccttgccggg agggggtggc tgccgtaggc
     3661 tgccggcgag cgagcggaaa ggagcacgac gatgacgacg agcggtcagg gcggcgaaga
     3721 gtatttcatc gacagccccg cgctgcgggg attcgtgggc gccgtgcgcg ccgcgatcgc
     3781 ggaggcggac agtcccggcc cgctggtcga gcggctgcgg ccccccttcg cccggctgct
     3841 ggccgatcaa gcgtggctgc cggcggagtt tgcccagccg gacgagacca gcggcatggg
     3901 cggcggcatc ggccagtatc tcctctaccg ctcggagagt ccggtcttca ccatcttctc
     3961 gctggtcgtg ccctccggtg ccatgacccc ggtccacgac cacctggcct gggggctgat
     4021 cggcctctac cgcggcgagc agcgggagga gatgtacgcg ctgatggcgg gcaaccccga
     4081 cgcgggcgag gccaagatcg agctgctgga ggagcggcgc gtcggccccg gcgacttcta
     4141 cgccatcctg ccgccggaca acgacatcca ccgcgtgacg acgacctcag ccggaccttc
     4201 gatctcgatc cacctgctcg gcaacgacac cggctgcgtg ctgcgccaca cctacgacca
     4261 ggccgccgag accagccaca gcttccgctc cggctacagc aacgcgccat gtgaggcaga
     4321 cggcgaggcg ggctagccgc gccgggctgg ctccagcgcg tcggcgatct cgtcggccca
     4381 ggcgcggatg gcgtcccagt cgcggaagtc gccctccggc gccttgacca tgcgggccag
     4441 cagccgctcc ccggcgccca gctcgctcct gtccagctca c
//
LOCUS       DASUBI010000792         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1044113, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000792 DASUBI010000000
VERSION     DASUBI010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044113"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..618)
                     /locus_tag="VFU72_15490"
     CDS             complement(103..618)
                     /locus_tag="VFU72_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEU5424945.1"
                     /translation="MLETQYASIDMICPQCHSIWEAPFARVVNVGTDPDGRMGALLGT
                     MHKSHCPACKTPRDVEVIWDYYDPEQQLVIQVRPEWEFKAGGGEEWYWARYEDLVEKY
                     KDVDVRVDVVFGFQELIEKHLGGEAAVAEAKRAWEARVAAEREQGQAQAQETPEAAEG
                     AAEDEAAEEKS"
     gene            839..1144
                     /locus_tag="VFU72_15495"
     CDS             839..1144
                     /locus_tag="VFU72_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424946.1"
                     /translation="MAERHIPAIGNQVLVSGKKKVGMVSALLTDGQVQEPTAFVVKYG
                     LLHKKTKRIPLAQIKWINAENVVLELTDRQFAALPEWQEGEAAAAAEPGSASGPTPA"
     gene            complement(1286..1810)
                     /locus_tag="VFU72_15500"
     CDS             complement(1286..1810)
                     /locus_tag="VFU72_15500"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEU5424947.1"
                     /translation="MTKNDQVRIQPGAQVVGSDNQYAGVVAQLRPEQQDMLVVDRRTG
                     GDVYIPFSAIQGLRGDTVVLNLAAADVDLRNWPHPATAGTVGGSPARPGPAGTGMPFG
                     EPGWPTFEGTGRDNPGPGSSNLVSDKKADFPNTPTADSLSRNPAPRPIDLEEEINENW
                     VTGAEEEPPPRREP"
     gene            1965..2270
                     /locus_tag="VFU72_15505"
     CDS             1965..2270
                     /locus_tag="VFU72_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424948.1"
                     /translation="MDPEQQMREMAIQWGSPVFDSEGNRIGTVEKVYRNADYGVPPPE
                     AAPKVIEPYMEIKTSREMLWVPFSQISRITPTGVTLNLLQNRIHELGFDEQPGFLQG"
     gene            2338..3423
                     /locus_tag="VFU72_15510"
     CDS             2338..3423
                     /locus_tag="VFU72_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424949.1"
                     /translation="MAARHAGARPRPEETQETAAEEAAEAAGLRYVTDDSPGIRRRRT
                     GHGFVYVDATGGRVSDEKTLARIRALAIPPAWTRVWICRAPNGHIQATGRDKKGRKQY
                     RYHDRWRAVRDATKYERMIAFGQALPRIRRGVERDLARRGLPREKVLAAVVRLLDETH
                     IRIGNAEYARENNSYGLTTLRDRHVKIKGATMEFHFVGKSGKKHEVILQDRRLAGIVK
                     RCQDIPGYDLFQYLDTEGERQTIHSEDVNDYLRALSGEEFSAKDFRTWAGTVLTLQAL
                     RKRGAAEGRRQAETNVREAIKATARVLGNTPAVCRRSYVHPAVINHYLAGQLAQLNQV
                     GRPRRGLRRAEQILLHLLEQEEQAPAA"
     gene            3518..3946
                     /locus_tag="VFU72_15515"
     CDS             3518..3946
                     /locus_tag="VFU72_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5424950.1"
                     /translation="MTSITRQQVGDGLSPAPILIIDDDEGIRDLVTLALGDEGYQVLA
                     AANGAAALELLRQERERPGVILLDTRMPVMDGAAFASAYRGEALPHAPIILLTAAAEA
                     DTIAEELEADAVLQKPFDLDELLSLIDRFNPAPGATSPGS"
     gene            complement(4008..4343)
                     /locus_tag="VFU72_15520"
     CDS             complement(4008..4343)
                     /locus_tag="VFU72_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424951.1"
                     /translation="MDAGAQAGRAARDRWLRRAEQERAETGEIVVNRGRLDWGTADVD
                     YYTLQPSPAQWVAVAVVPVIPATEGTSRMIVGTGASEFAAVEALGERLGRLLPPAEQL
                     AQPSDWFGD"
     gene            complement(4435..5451)
                     /locus_tag="VFU72_15525"
     CDS             complement(4435..5451)
                     /locus_tag="VFU72_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5424952.1"
                     /translation="MPSVSTPLLLLILIIMFGVIFDFINGFHDTANAIATSVATRVLS
                     PGQAVAMSAVFNFVGALSGTAVATTVGKGIIAPANSTQLVVIAALVSAITWDLLTWYF
                     GIPSSSSHALIFSIVGTGVAAAGFDAIETGGLSKTFQGLVFSPLFGFAVGFAVMIGLF
                     WIFARVRPRVVNSIFGKAQILSAAYMSYSHGLGDAQKTMGIITMALASYYGWSGGEWH
                     VPIWVIATAATAMSLGTAVGGWRVINTMGFKVVELRPINGFAAETAAASIIEIAARLG
                     IPTSTTHVISSAILGVGSTRRVSAVRWGIAGRILAAWVITIPACIAMGWGIYEALSLV
                     TGGH"
     gene            complement(5444..6085)
                     /locus_tag="VFU72_15530"
     CDS             complement(5444..6085)
                     /locus_tag="VFU72_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5424953.1"
                     /translation="MIYKRFLPRNEEFYAYFNEIASTNAEAAQLLYEVLESMDDVERR
                     VRHLRDLEHRADETAHRIYNALDSTFVTPIDREDIRTLTSQLDDFMDFIEEAGRRVLL
                     YRFDSSTDIARMLGRLIVEQAEALKEGVPLLEHTKQEGQAIHRMILEVNRLENEADDG
                     LNQALGNLFDGVDDIPGMIRSIRWGEVYQILEDATDRAENVANTIEGITLKYA"
     gene            complement(6224..6817)
                     /locus_tag="VFU72_15535"
     CDS             complement(6224..6817)
                     /locus_tag="VFU72_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5424954.1"
                     /translation="MEFADTVRKRRIVRHFRPDPIDPAAVDRMLELARRAPSAGYTQG
                     QAFIVITDPELRRQVARCCDPGGQHAAHFGYDWVAEAPVQIIPCAGEAAYHARYQEPD
                     KLQPDGSEVVWPVPFWFIDIGAAIMVLLLAVVDEGLAAGYAGIPDTPGLCDLLGIPEG
                     VYPVGVIPVGHPAPDVPSPSLKRGRKPLATVVHRERW"
BASE COUNT         1157 a         2467 c         2379 g         1100 t
ORIGIN      
        1 cagtgggctt cgcggactca gcccgagaat tccattctcg ggcgccacgc ggcactcacg
       61 caccggctcc acgaccaacc gtccaccaag ccgccacacg cctcaggact tctcctccgc
      121 tgcctcatcc tcggccgcgc cctccgccgc ctccggagtc tcctgtgcct gcgcctgccc
      181 ctgctcgcgc tcagcggcca cgcgcgcttc ccacgcgcgc ttggcctcgg ccacggccgc
      241 ctctcccccc aggtgcttct cgatcaactc ctggaagccg aagacgacat cgacgcggac
      301 atcgacgtcc ttatacttct ccaccagatc ctcgtagcgt gcccagtacc attcttcgcc
      361 gccgccggct ttgaactccc actctggccg cacttgaatc acgagctgct gctcggggtc
      421 gtagtagtcc cagatcacct cgacatcgcg cggcgtcttg caggcggggc agtggctctt
      481 gtgcatcgtg ccgagcagcg cgcccatccg cccatcggga tccgtgccca cgttgacgac
      541 gcgcgcgaac ggcgcttccc agatcgaatg gcattggggg cagatcatat cgatcgatgc
      601 gtattgggtc tccagcacca gctcctccat caaggctcac ctgctcacaa gcgcgaacgc
      661 aacggtgcgc tgacgcaatt ataggatgcg gtcgccgcgg catccggcgc gcaacttata
      721 cgaagttaag ccgtgcttca cgccgggtta agcgttggag cccataccag agctagcgcg
      781 aaggcgccca ggcaaaacgc gatgaggtag gagtcgcctg gcggaaggat gagagacgat
      841 ggctgagcga cacatccccg cgattggcaa ccaggtactc gtctccggca agaagaaggt
      901 cggcatggtc agcgcgttgc tgaccgatgg ccaagtacag gagccgaccg ccttcgtcgt
      961 caaatacggc ctgctccaca agaagaccaa gcgtatcccg cttgcccaga tcaagtggat
     1021 caacgccgag aacgtcgtgc tcgagctgac cgaccgacag ttcgcggcgc tgcccgagtg
     1081 gcaagagggg gaggctgcgg cggcggcaga gccgggttcc gcctccgggc cgacacccgc
     1141 ctaagcgaac gctacgccta attcacctcc ttccatgtgc cgccgccgag gtgttccccc
     1201 gccccggcgg ctttttcccg ccccgcgcac gacacgacag cggccccgcg ctggtcgcga
     1261 ggccgctgtc gctcgttggg ctctgctagg gctcgcgccg cggcggcggt tcctcctcgg
     1321 cgccggtcac ccagttctcg ttgatctcct cttcgagatc gatcggacgc ggcgcggggt
     1381 tgcgcgacag tgagtcggcc gtcggcgtgt tcgggaagtc ggctttcttg tccgacacca
     1441 ggttcgacga gccaggcccg gggttgtcgc gcccagtgcc ctcgaaggtg ggccagcccg
     1501 gctcaccgaa gggcatgccg gtgccggccg ggccgggccg ggctgggctg ccccccaccg
     1561 tgccggccgt ggccgggtgc ggccagttcc gcaggtcgac atcggccgcc gccaggttga
     1621 gcacgacggt gtcgccgcgc agcccctgga tcgcgctgaa ggggatgtag acatcgccgc
     1681 cggtgcgccg gtccacgacc agcatatcct gctgctccgg gcgcaactgc gccacgaccc
     1741 ccgcgtactg gttgtcggag ccgacgacct gcgcccccgg ctgaatgcgg acctgatcgt
     1801 tcttggtcat ggtgcgctcc ttcatctgct ccgcgaagat ggctcgcatg ccgtgggctg
     1861 caatctctgt gccggtgccg aatcgtgggg cgcggtggaa cggcctttgc gccctggcat
     1921 ctcgccgcct ccgcgccgca gcggagcgcg gaaaggaggg tggcatggac ccggaacagc
     1981 agatgcgcga gatggcgatc cagtggggtt cgcccgtctt cgatagcgaa ggaaatcgca
     2041 tcggaacggt agagaaggtc taccgtaacg ccgactacgg cgtgccgccg cccgaggcgg
     2101 ctcccaaggt gatcgagccc tacatggaga ttaagacatc ccgggagatg ctctgggtgc
     2161 cgttcagcca gatcagccgc atcaccccca cgggcgtgac gctcaacctc ttgcagaacc
     2221 gcatccacga gctgggattc gatgagcaac ccggcttctt gcaggggtag caggtagaca
     2281 ggggacagcc gcgcgacacg gcagaggacc gagatcgacg atcgcaggag gagcatcgtg
     2341 gcagcgcggc acgccggggc gcggccccgg ccggaggaga cgcaggagac cgcggccgag
     2401 gaggcggccg aagccgccgg cctacgctat gtcaccgacg acagcccggg catccgccgc
     2461 cggcggaccg ggcacggttt tgtctacgtc gacgcgacgg gcgggcgcgt cagcgacgag
     2521 aagacgttgg cgcgcatccg cgcgctggcc atcccgccgg cctggacgcg cgtctggatc
     2581 tgccgcgcgc ccaacgggca catccaggcc accgggcggg ataagaaggg ccgcaagcag
     2641 tatcgctatc acgaccgctg gcgcgcggtg cgcgacgcca ccaagtacga gcgcatgatc
     2701 gccttcggcc aggcgctgcc gcgcatccgg cgcggcgtcg agcgcgacct ggcccgccgc
     2761 ggcctgcccc gcgagaaggt gctggccgcc gtcgtgcgcc tgctggacga gacccacatc
     2821 cgcatcggca acgccgagta cgccagagag aacaactcct acggcctgac gacgctacgc
     2881 gaccggcacg tcaagatcaa gggcgccacg atggagttcc acttcgtcgg caagtccggc
     2941 aagaagcacg aggtcatcct gcaggatcgc cgcctggcgg gcatcgtcaa gcgctgccag
     3001 gacatccccg gttacgatct cttccagtac ctcgataccg agggcgagcg tcagacgatt
     3061 cactcggagg acgtgaacga ctacctgcgc gcgctcagcg gcgaggagtt cagcgccaag
     3121 gatttccgca cctgggccgg cacggtgctg acgttgcagg cgctgcgtaa gcgcggggcg
     3181 gccgagggcc gcaggcaggc cgagacgaac gtgcgcgagg cgatcaaggc gaccgcccgg
     3241 gtgctcggca acacgcccgc cgtctgccgc cgcagctacg tccaccccgc cgtgatcaac
     3301 cactacctcg ccggccagtt ggcgcagttg aatcaggtgg gccgccctcg gcgcggcctg
     3361 cgccgcgccg agcagatcct gctccacctg ctcgagcagg aggagcaggc accggccgcc
     3421 tgagcgggct ggtgccatct cgctcgcgac cggacgcgcg cggggtgcta tactgcatcc
     3481 agcgagtacg actggcgaat gcgaagatcg tggccgtgtg acgtcgatca cgcgccagca
     3541 ggttggggat gggttgagcc cggcaccgat cctgatcatc gacgacgacg agggcattcg
     3601 cgatctggtg accctggcgc tcggcgacga gggctatcag gtgctagccg ccgccaatgg
     3661 cgccgccgcg ctcgaactgc tgcggcagga gcgggagcgg ccaggggtga tcctgctcga
     3721 cacgcgcatg ccggtgatgg acggcgcggc cttcgccagc gcctaccgcg gcgaggcgct
     3781 gccgcacgcg ccgatcatcc tgctgaccgc cgccgccgag gccgatacga tcgccgaaga
     3841 gctggaggcc gacgccgtcc tgcagaagcc ctttgatctc gacgagttat tgagcctgat
     3901 cgaccgcttc aacccggcgc ccggcgctac ctcgcccggg tcgtagtcgt catctcatgc
     3961 tgtgcgcgct tgaccggttt gcccctacct tgacgggcaa acgggcgcta gtcgccgaac
     4021 cagtcgctcg gctgggccag ttgctccgcg ggcggcagca ggcgccccag tcgctcgccc
     4081 agggcctcga ccgccgcgaa ctcactcgcg ccggtgccga cgatcatccg gctggtcccc
     4141 tccgtcgccg ggatgaccgg cacgaccgcg accgcgaccc actgggccgg ggatggctgc
     4201 agggtgtagt agtccacgtc tgccgtgccc cagtcgagcc tgccgcggtt gacgacgatc
     4261 tcgcccgtct ccgcgcgctc ctgctcggca cgccgcagcc agcggtcgcg cgccgcgcga
     4321 cccgcctgcg ctcccgcgtc catcgtgccc ccttgcccgg cgcgccccgc gccgccctgc
     4381 acaaccttgg gttcacctca accagaaaac gaccgtagct caactctcgc aagatcagtg
     4441 cccgccggtc accagcgaga gcgcctcgta gatgccccag cccatggcga tgcaggctgg
     4501 gatggtgatg acccaggccg ccaggatgcg gccggcgatc ccccagcgta cggccgagac
     4561 gcgccgggtc gagccgacgc ccaggatggc cgaggagatg acgtgggtgg tcgaggtcgg
     4621 gatgccgagc cgggccgcga tctcgatgat gctggcggcc gcggtctcgg ccgcgaagcc
     4681 gttgatcggg cgcagctcca ccaccttgaa acccatggtg ttgatcacgc gccagccgcc
     4741 gaccgcggtt cccaggctca tggcggtggc ggcggtggcg atcacccaga tgggcacgtg
     4801 ccactcgccg ccgctccagc cgtagtagct ggcgagcgcc atggtgatga tgcccatcgt
     4861 cttctgcgcg tcgccgagcc cgtggctgta cgacatgtag gctgccgaga gaatctgcgc
     4921 cttgccgaag atactgttga ccacgcgcgg ccggacgcgc gcgaagatcc agaagagccc
     4981 gatcatcacc gcgaagccga cggcgaagcc gaagagcggc gagaacacca acccctggaa
     5041 ggtcttgctc aggccgccgg tctcgatggc gtcgaacccg gcggcggcga cgccggtgcc
     5101 gacgatcgag aagatcagcg cgtggctgga gctggagggg atgccgaagt accaggtcag
     5161 cagatcccag gtgatggccg agaccagcgc ggcgatcacc accagttggg tcgagttggc
     5221 cggggcgatg atccccttgc ccaccgtggt cgccaccgcc gtgcccgaga gcgcgccgac
     5281 gaagttgaag acggccgaca tcgcgaccgc ttgaccgggg gagaggaccc gcgtcgcgac
     5341 cgaggtcgcg atggcgttgg ccgtgtcgtg gaagccgttg atgaaatcga agatcacgcc
     5401 gaacataatg atcaggatca acaggagcag cggcgtgctc acgctaggca tacttcagcg
     5461 tgatcccttc gatggtgttg gccacgttct ccgcccggtc ggtcgcatcc tccagaatct
     5521 gatagacctc gccccagcgg atcgagcgga tcatgccggg gatgtcgtcc acgccgtcga
     5581 agaggttgcc gagcgcctgg ttcagcccgt cgtccgcctc gttctccagc cggttgacct
     5641 ccaggatcat gcggtggatc gcctggccct cctgcttggt gtgctccagc aggggtacgc
     5701 cctccttgag cgcctcggcc tgctccacga tcagccggcc tagcatgcgc gcgatgtcgg
     5761 tcgaggagtc gaagcggtag aggaggacgc ggcgcccggc ctcctcgatg aaatccatga
     5821 agtcgtccag ttgcgaagtc agcgtgcgga tatcctcgcg atcgatcggc gtgacgaagg
     5881 tgctgtcgag cgcgttgtag atgcggtggg ccgtctcgtc ggcccggtgc tccaggtcgc
     5941 gcaggtggcg cacgcggcgc tccacgtcgt ccatcgactc cagcacctca tagagcaact
     6001 gcgcggcctc ggcgttcgtg ctggcgatct cgttgaagta ggcgtagaac tcctcgttgc
     6061 ggggcaggaa acgtttgtag atcatcgcag ctccttcacc gctggtcgcg cctggcgcgg
     6121 cattacttcc ctgccgcatt tttcacaatc ttccgctgct cggcaacagc ggggagcgat
     6181 gaccaagtct agcggagtcc gcggcgacgg gtgacgggcc gctctaccag cgctcgcggt
     6241 gcacgaccgt cgccagcggc ttacggccgc gcttgagcga gggcgagggc acgtccggcg
     6301 cggggtggcc gaccgggatc acgccgacgg ggtagacccc ttcggggatg cccagcaggt
     6361 cgcacaaccc cggtgtatcg gggatgccgg cgtagccggc cgccagcccc tcgtcgacca
     6421 ccgccagcag gagcaccatg atcgcggcgc cgatgtcgat gaaccagaag gggaccggcc
     6481 acacgacttc gctgccgtcg ggctggagct tgtccggctc ctggtagcgc gcgtggtagg
     6541 cggcctcgcc ggcacagggg atgatctgca ccggcgcttc ggcgacccag tcgtagccga
     6601 aatgggcggc gtgctggccg ccggggtcgc agcagcgggc cacctgccgg cgcagctccg
     6661 ggtcggtgat gacgatgaag gcctgcccct gagtatagcc ggcgctgggc gcgcggcgtg
     6721 ccagctcgag catgcggtcc accgccgccg ggtcgatcgg gtcgggcctg aagtggcgca
     6781 cgatgcgccg cttgcggacg gtgtcggcga actccatggc tggcctgtcc ttcctggcgc
     6841 gcttttgtgc cggccgcacc ggtcgtggca gaataagccg cgtccccggc cgcggcaaga
     6901 gcggcccaac ccacccacac accgtacacg ctcaacgcaa ggagcatgca tgcccacatc
     6961 caccccgctg gctctcggtc tcgatgtcta cggtacgctg gttgacctgc tggccctgcg
     7021 ccgcgtgctc ggctcgctgg tcggcgacct ggccgagacc ttcaccatac actggcgcca
     7081 gaagcatgtc gagtacgcct tcc
//
LOCUS       DASUBI010000793         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1335642, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000793 DASUBI010000000
VERSION     DASUBI010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335642"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFU72_15540"
     CDS             <1..242
                     /locus_tag="VFU72_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5424955.1"
                     /translation="AVDTTGAGDSFVAGLLTGLLEADLRLDPATIARAVRLGTACGAL
                     TTTRRGAIPALPNRAAVERLLATSHQERNPGCATP"
     gene            227..877
                     /locus_tag="VFU72_15545"
     CDS             227..877
                     /locus_tag="VFU72_15545"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEU5424956.1"
                     /translation="MRYALSWSGGKDSTLALDRARRAGLDVACLFNIYERSSGRVRFH
                     GVRAELIAAQANALGLPLVAAHTHPDDYETVFLRLLDQFRSEGIGGVMFGNIHLADIR
                     AWYEERTTSRGFAHTEPLWGEAPGDLIREFVARGYLARIVSIDLARARPEWLGAPLDL
                     DLIHSIEAEPAVDVCGERGEYHTFVSAGPLFQRPVPVQPGEAIEMEGHRSIDLQLE"
     gene            980..1189
                     /locus_tag="VFU72_15550"
     CDS             980..1189
                     /locus_tag="VFU72_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424957.1"
                     /translation="MTTEISHQALPLEELSPEEARRFFDRQVRRSLQMSGDEFARRWE
                     AGEIDEPDRPEVQLLALHLPFARAR"
     gene            1321..2145
                     /locus_tag="VFU72_15555"
     CDS             1321..2145
                     /locus_tag="VFU72_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5424958.1"
                     /translation="MARTLVLANQKGGVGKTTTAVNLAAELASQGHRVLLIDIDPQGN
                     ATSSLGVEKNTVARSTYDVLIEGEPIAGCRVAGVRPRLDLLPANGELAAAEVELVGLR
                     RREFRLREALEREAGRYDVVVIDCPPSLGLLTVNALSAADEVVVPIQCEYLALEGLMQ
                     LINTIDLVKRRLNPALDVLAVVLTMFDARTRLSAQVVEDVQRFFPSRTLESVIPRTVR
                     LAEAPSYGQTIYEYDSASRAASAYRDASQEIAARLGLQSPAEPPLLTAARRREPWT"
     gene            2213..3748
                     /gene="guaB"
                     /locus_tag="VFU72_15560"
     CDS             2213..3748
                     /gene="guaB"
                     /locus_tag="VFU72_15560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5424959.1"
                     /translation="MTIIDNPHRTIVQSTGLLDEQSEEKILGVGLTFDDVLLVPAASE
                     ILPKDADTRARLTRNISLNIPILSAAMDTVTEGRMAIAMAREGGLGILHRNLSIEDQV
                     AEVDKVKRSESGMIVEPVTLQPEDQVRDALAVMERYHISGVPITDPQGKLVGILTNRD
                     LRFEEDLDQPIAALMTHEHLITAPVGTSLDEAREMLHENKVEKLPVVDEHGFLKGLIT
                     VKDIQKRIQYPNATKDSRGRLRVGAALGVAGDALERAAALVAAGVDVLVIDTAHGHSR
                     GVIEMVRTVKTRWDVEVIAGNIATAGGAEALITAGADAIKVGIGPGSICTTRIVAGTG
                     VPQITAIMNVAPVAARYGVPVIADGGIQFSGDIAKAIAAGADTVMLGGLLAGVDESPG
                     DVILYPGERFKEYRGMGSLGAMKGRSFSKDRYFQEDVTGVAKLVPEGIEGRVAYKGPL
                     ATIIYQLVGGLRAAMGYCGAASVPQLQRDAHFVQITAAGLRESHPHDVMITKEAPNYR
                     VSR"
     gene            4006..>4799
                     /locus_tag="VFU72_15565"
     CDS             4006..>4799
                     /locus_tag="VFU72_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5424960.1"
                     /translation="MRIAQIAPLWEGVPPRYYGGTERVVSALTEELVARGHEVTLFAT
                     ADSRTEARLAPMAPAGLRLGGAKDGNALHVAMLAEAFEQAERFDVIHSHVDVLGFPFA
                     RHSPTPVVHTLHGRLDYPEQHRVLACYPDLQFVSISHSQRRPVRDLPLTWLGTVHNGI
                     QADHFRPRLTPITDRADRYLVFLGRISPEKGPCQAIEVARRVGLPLKIAAKIDPADQE
                     WAAEHFLPLLDGPGVEFLGEVDDRGKTELLAGAYAYLFPINWPEPFG"
BASE COUNT          773 a         1691 c         1618 g          717 t
ORIGIN      
        1 cggcggtcga tacgaccggc gcgggcgata gcttcgtcgc ggggctcttg actggactgc
       61 tggaggcgga tctgcgtctc gatcccgcaa cgatcgcacg ggccgtgcga ctgggcaccg
      121 cctgcggcgc gctgaccacc acccgccgcg gcgcgatccc cgcgctgccg aaccgcgccg
      181 cggtcgaaag attgctggcg accagtcatc aggagaggaa tccagggtgc gctacgcctt
      241 gagctggagc ggcggcaagg actcgacgct ggcgctcgac cgggcccgcc gcgcggggct
      301 cgacgtcgcc tgcctgttca acatctacga gaggagcagc gggcgcgtgc gcttccacgg
      361 cgtgcgcgcg gagctgatcg cggcacaggc gaacgcgctc ggcctgccgc tggtggccgc
      421 gcacacccac cccgacgact acgagaccgt cttcctgcgc ctgctcgacc aattccgctc
      481 cgagggcatc ggcggcgtca tgttcggcaa catccacctg gccgacatcc gtgcctggta
      541 tgaggagcgc acgaccagcc gcggtttcgc ccatacggag ccgctctggg gcgaggcgcc
      601 tggcgacctg atccgcgagt tcgtggcgcg cggctacctg gcccgcatcg tcagcatcga
      661 cctggcccgc gcccggccgg agtggctcgg cgcgccgctc gatctcgacc tgatccactc
      721 gatcgaggcc gagcccgccg tcgacgtctg cggcgagcgc ggcgagtacc acaccttcgt
      781 ctccgccggc ccgctctttc agcggccggt gccggtccag cctggcgagg cgatcgagat
      841 ggaggggcac cgctccattg atctccaact ggagtaggtg ccgcaatgca tccccagcgt
      901 cgggacgatg agcccctggc cgagcgctcg cgactgatcg ttcgacggaa taagcttgat
      961 ggtcgaaagg aggagcgtca tgacaaccga gatcagccat caggcgctcc ccctggaaga
     1021 gctctccccc gaggaggcac gccggttttt cgaccgccag gtccgtcgct ctttgcagat
     1081 gagcggcgac gagttcgcgc gccgctggga agccggagag atcgacgagc cggaccgacc
     1141 cgaggtccag ctcctcgcct tgcacctccc ctttgcccgc gcccgctaga cgcgccgatg
     1201 cccgatcgca cgccccgcga ggctgtcgag acctaccccc aagcagggcc gcgagcgctt
     1261 gtccggacac cggccaaggg gtacaattgg gcaattaact cggaggcgac ggagcggcgc
     1321 ttggcacgca cgctggtact ggcaaatcaa aagggcggcg tcgggaagac gactacggct
     1381 gtcaacctgg cagccgagct tgccagtcag ggccaccggg tactcctgat cgatatcgat
     1441 ccccagggca acgccaccag cagcctcggc gtcgagaaga acacggtggc gcgttccacc
     1501 tacgacgtgc tgatcgaggg cgagccgatc gccggctgcc gtgtcgccgg cgtgcggccc
     1561 cggctcgacc tgctgccggc caacggcgag ctggccgcgg ccgaggtcga gctggtcggg
     1621 ctgcggcgac gcgagttccg gctgcgcgag gcgctggagc gggaggccgg gcgctacgac
     1681 gtggtggtca tcgactgccc cccgtcgctc ggcctgctga ccgtcaacgc cctgagcgcc
     1741 gccgacgagg tggtggtccc gatccagtgc gagtacctgg ccctggaggg gctgatgcag
     1801 ttgatcaaca ccatcgacct ggtcaagcgg cggctcaacc ccgcgctcga cgtgctggcg
     1861 gtggtgctga ccatgttcga cgcccgcacg cgcctgtcgg cccaggtggt cgaggacgtg
     1921 cagcgcttct tccccagccg gacgctggag agcgtcatcc cgcgcaccgt gcgtctggcc
     1981 gaagctccga gttacgggca aaccatctac gagtacgata gcgcctcccg cgcggccagc
     2041 gcctaccggg acgccagcca ggagatcgcg gcccggctcg ggttgcagtc gccggcggag
     2101 ccgccgctgc tgaccgccgc gcgccgccgc gagccatgga cctagccctg tcgtcgttgt
     2161 ccgcgagacc gtgaccaccc gcgcagggga atggaaagaa agaggtgaga cgatgacgat
     2221 catcgacaat ccgcatcgca ccatcgtcca atccaccggg ctgctcgatg agcagtccga
     2281 ggagaagata ctcggcgtcg gtctgacctt tgatgatgtc ctgctggtcc ccgccgcctc
     2341 ggaaatcctg ccgaaagacg ccgacacccg cgcccgcctg acccgcaaca tctcgctgaa
     2401 catcccgatc ctctccgccg ccatggatac cgtgacggaa gggcgcatgg cgatcgccat
     2461 ggcccgcgag ggcggcctgg gcatcctgca ccgcaatctc tcgatcgagg accaggtggc
     2521 cgaggtcgac aaggtcaagc gcagcgagtc gggcatgatc gtcgagccgg tgacgctcca
     2581 gccggaggac caggtgcgcg acgcgctggc ggtgatggag cgctaccaca tctccggcgt
     2641 gccgatcacc gatccacagg gcaagctggt cggcatcctg accaaccgcg acctgcgctt
     2701 cgaagaggac ctcgaccagc cgatcgcggc cctgatgacc cacgagcacc tgatcaccgc
     2761 gccggtcggt acaagcctcg acgaggcgcg cgagatgctg cacgagaata aggtcgagaa
     2821 gctgccggtg gtcgacgagc acggcttcct caaggggctg atcacggtca aggacattca
     2881 gaagcgcatc cagtacccca acgccaccaa ggattcacgc ggccggctgc gcgtcggcgc
     2941 cgcgctgggg gtggcgggcg acgcgctgga gcgtgccgcc gcgctggtcg ccgcgggggt
     3001 cgacgtgctg gtgatcgaca ccgcgcacgg tcactcgcgc ggggtgatcg agatggtgcg
     3061 cacggtcaag acgcgctggg atgtcgaagt tatcgccggc aacatcgcca ccgccggcgg
     3121 cgccgaggcg ctgatcactg ccggggccga cgcgatcaag gtcggcatcg gccccggttc
     3181 gatctgcacc acccgcatcg tggccggcac cggtgtgccg cagatcaccg ccatcatgaa
     3241 cgtcgccccg gtggcggcgc gctacggcgt gccggtcatc gccgacggtg gcatccagtt
     3301 ctccggcgat atcgccaagg cgatcgcggc gggggccgat acggtcatgc tcggcgggct
     3361 gctggccggg gtcgacgaga gccccggcga cgtcatcctc tatccgggcg agcgcttcaa
     3421 ggaataccgc ggcatgggct cgctcggcgc gatgaagggc cgctccttca gcaaggaccg
     3481 ctacttccag gaggatgtca ccggcgtggc caagctggtg ccggagggaa tcgaagggcg
     3541 agtggcctac aaggggccgc tggccaccat catctaccag ctcgtcggcg gcctgcgcgc
     3601 ggccatgggc tactgcggcg cggcctcggt gccgcagctg cagcgcgatg cccacttcgt
     3661 ccagatcacc gccgccggtc tgcgtgagag ccacccccac gacgtgatga tcaccaaaga
     3721 ggcgcccaac taccgcgtct cgcgctgatt ccgcgccgtc tccacgggcc gcccagtggg
     3781 cggcccgcct gtcctcatcc ctggtagtga ctccgcggca tatttcgtgc ggttatgacc
     3841 gttcgctggt cggaatgcat cgacccgcgc agcgccatcg gtaccacgtt tggttgccgg
     3901 tggtgcctgg tcacgcggag gagcgtgcca tcggcaggac aaggttgtcc cgttgacacg
     3961 cccgacgctg cctgctgaca atcacttgcc tgggaagggc acgacatgcg catcgcacag
     4021 attgctccct tgtgggaagg ggtgccgccg agatactacg gcggcaccga gcgggtcgtc
     4081 tccgccttga ccgaggagct ggtggcacgt ggccatgagg tgacgctctt cgcgaccgcc
     4141 gattcgcgca ccgaagcacg gctggcgccg atggcgccgg ctggcctgcg gctcggcgga
     4201 gccaaggacg gcaacgcatt gcacgtcgcc atgctggccg aggcgttcga gcaggccgag
     4261 cggttcgatg tgatccactc gcacgtcgac gtgctcggct tccccttcgc gcgtcactcc
     4321 cccaccccgg tggtgcacac cctgcacggc cggctcgact accccgagca gcaccgcgtg
     4381 ctggcctgct acccggacct ccagttcgtg agcatcagcc acagccagcg ccgccccgtg
     4441 cgcgatctgc cgctgacatg gctcggcacc gtccacaacg gcatccaggc cgaccacttc
     4501 cggccccggc tgacgcccat caccgatcgc gccgaccgct acctggtctt cctggggcgc
     4561 atcagtcccg agaaggggcc ctgccaggcg atcgaggtag cgcgccgggt cggcctcccg
     4621 ctcaagatcg cggccaagat cgaccccgcc gaccaggagt gggccgccga gcacttcctg
     4681 ccgctgctcg acggccccgg tgtcgaattc ctgggcgagg tcgatgatcg cggcaagacg
     4741 gagctcctgg ccggcgccta cgcctacctg ttcccgatca actggccgga gccgttcgg
//
LOCUS       DASUBI010000794         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1070179, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000794 DASUBI010000000
VERSION     DASUBI010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070179"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFU72_15570"
     CDS             <1..988
                     /locus_tag="VFU72_15570"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5424961.1"
                     /translation="AHGAMPTVHPQSLGLWRAGNSNEYVERSFDAADIVLGVGVRRGT
                     ESDRGLRDRLGDKFVILDAVDAPATDGQLEAPSLDELAAILERLIGATRERPADPQAR
                     AEWAEARRFFQLGLDAEIARYAERRPWPIGLALASLARQMTPETLVVSDVSNVKIWAP
                     LQLPTDNSLSHLQSGSWGAMGYVVPGVLGAALARPDRKVVGIVGDAAFLMGSNDFGTI
                     CSLGLPVVIAVHADQQIGMIHYSLSRAFGRTYKTEVPAVDFMRYAEAFGARGLRVEEP
                     AELDAAWREALAADGPVLLELRAGHDFPRPYPIQRIIDQGRERATEGTLTGN"
     gene            complement(1037..1435)
                     /locus_tag="VFU72_15575"
     CDS             complement(1037..1435)
                     /locus_tag="VFU72_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5424962.1"
                     /translation="MPQGTVPERYRDLLESTALGHLATIGRDGHPQVNPVWFISDGAH
                     IFLSFKADTRKLRNVRANPFVAMSIADPANPFRYVELRGEVVALELFDTLAWANQLAR
                     KYTGADFTSGRDGEHRYKVTIRVDSWTGQG"
     gene            1803..>2535
                     /locus_tag="VFU72_15580"
     CDS             1803..>2535
                     /locus_tag="VFU72_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5424963.1"
                     /translation="MDLRYTAEDEAFRADVRAWLAEHAPREPLRSVAEKKAWHRKLYQ
                     AGYLGMGWPRAYGGRAARPIEQAIVVEEMARGHAPASINWAGLGLVGPTLIHHGSDAQ
                     RERYLRNILLGDEVWCQLYSEPNAGSDLASLQCRADIDGDEFVVNGQKIWTSTAPEAD
                     MGILLVRTDRQAPKHQGISYLIVDMRWPGIEVRPLRQITGIADEFAEVFFSNVRVPAD
                     NLIGELNAGWRIAQTTLSYERGGDTM"
BASE COUNT          394 a          913 c          842 g          386 t
ORIGIN      
        1 cgcccacggc gccatgccga ccgtccaccc gcagtcgctt ggcctctggc gcgccggcaa
       61 cagcaacgag tacgtcgagc ggagcttcga cgcagccgat atcgtgctcg gcgtcggcgt
      121 ccgtcgcggc accgaaagcg atcggggtct gcgcgaccgc ctcggcgaca agttcgtcat
      181 cctcgacgcg gtcgatgccc ccgcaactga cggccagctc gaagcgccgt ccctcgacga
      241 actggccgcc atactggagc ggctgatcgg cgcgaccagg gagcggccgg ccgatcccca
      301 ggcgcgcgcg gagtgggcgg aggcccggcg gttcttccag ctcgggctcg acgccgagat
      361 cgcgcgctac gccgagcgtc ggccctggcc catcggcctg gcgctggcca gcctggcccg
      421 ccagatgacg ccggagacgc tggtcgtcag cgacgtcagc aacgtcaaga tctgggcgcc
      481 gctgcagttg cccaccgaca actcgctgtc ccatctacag tccggaagct ggggcgccat
      541 gggctacgtc gtgcccggcg tgctcggcgc ggcgctggcg cgaccggacc gcaaggtggt
      601 gggcatcgtc ggcgacgcgg ccttcctgat gggctcgaac gacttcggca ccatctgctc
      661 gctcggcttg ccggtggtga tcgccgtaca cgccgaccag cagatcggca tgatccacta
      721 ttctctcagc cgcgccttcg gccgcaccta caagaccgag gtgcccgccg tcgactttat
      781 gcgctacgcg gaagccttcg gcgcgcgcgg cctccgcgtc gaggagccgg ccgagctgga
      841 cgcggcctgg cgcgaggcgc tggccgccga cggcccggtc ttgctggagc tgcgcgccgg
      901 gcacgatttc ccgcgtccct accccatcca gcgcatcatc gaccagggcc gcgagcgcgc
      961 gacagagggc actctcaccg gaaattgagc aggcgagatg ttcgtctcca ggatcgctgc
     1021 accctccctg cccgcgtcac ccctgtcccg tccacgagtc gacgcggatc gtgaccttgt
     1081 agcgatgctc gccgtcgcgg ccggaggtga agtcggcgcc ggtgtatttg cgcgccagtt
     1141 gattagccca ggcgagcgta tcgaagagct ccagcgcgac gacctcgccg cgcagctcca
     1201 cgtagcggaa cgggttcgcc ggatcggcga tcgacatggc cacaaaggga ttcgcgcgca
     1261 cgttgcgcag cttgcgcgtg tcggccttga agctcaagaa gatgtgcgcc ccgtccgaga
     1321 tgaaccagac cgggttgacc tgcgggtggc catcccgtcc gatagtcgcc agatggccca
     1381 gcgcggtact ctccaacaga tcccgatagc gctccggcac ggtcccttgt ggcacgcccc
     1441 gctccttcca ctctagccgc tttcttctcg acgaccagta tcagactccg ccgccgtcct
     1501 ggccaccgcc gtgacttcac acgcgcgcga gagcgtcggt gacctatggg tatgggactg
     1561 ttaggcgccc ttggcctggc ggtcgctgtc acccggggct gatgggagat ccttcggctg
     1621 cggcctcagg atctctcccg cgctgcagac gtcgctcgac tctggaccag cacgcacgag
     1681 tccgccaggc accccgcccg cgccacgcgt cgtcaggcaa cgcgactcgg gctatactgc
     1741 catccacggc agctcaattc gtcccgccgc gatcaaccat cagtgcctgg agatggacag
     1801 cgatggacct gcgctatacg gcagaggatg aggcatttcg agccgacgtc cgtgcctggc
     1861 tggccgagca cgcgccgcgc gagccgctgc ggtccgtggc cgagaagaag gcctggcacc
     1921 gcaagctcta ccaggccggc tacctgggca tgggctggcc gcgggcctac ggcgggcgcg
     1981 cggcgcggcc gatcgagcag gcgatcgtgg tcgaggagat ggcgcggggt cacgcgccgg
     2041 cctcgatcaa ctgggcgggc ctcgggctgg ttggaccgac cctgatccac cacggcagcg
     2101 acgcccagcg cgagcgctac ctgcgcaaca tcctgctcgg cgacgaggtc tggtgccagc
     2161 tctactccga gcccaacgcg ggctccgatc tggccagcct gcagtgtcgc gccgacatcg
     2221 acggcgatga gttcgtggtc aacgggcaga agatctggac cagcaccgcg ccggaggccg
     2281 acatgggcat cctgctggtg cgcaccgacc gccaggcgcc caagcaccag ggcatctcct
     2341 acctgatcgt cgacatgcgc tggcccggca tcgaggtgcg cccgctgcgc cagatcaccg
     2401 ggatcgccga cgagttcgcc gaggtcttct tcagcaacgt gcgcgtgccg gccgacaacc
     2461 tgatcggcga gctgaacgcc ggttggcgca tcgcccagac gaccctctcc tacgagcgcg
     2521 gcggcgacac catgg
//
LOCUS       DASUBI010000795         4370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_191087, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000795 DASUBI010000000
VERSION     DASUBI010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191087"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU72_15585"
     CDS             <1..297
                     /locus_tag="VFU72_15585"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5424964.1"
                     /translation="TREPWLAIDPKGVVGEPAAEVGPLLLNPRPLLLSWPHPERVLAR
                     RLDILSEALGIERERLWAWGLAHAMLSACWSVEDFGAGWEYAIAMAELLAGMRG"
     gene            complement(557..1018)
                     /locus_tag="VFU72_15590"
     CDS             complement(557..1018)
                     /locus_tag="VFU72_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424965.1"
                     /translation="MSEQQHQQQHESGNKYLIDRAENLTKEDKAYLEQYADKLSRTTL
                     TARWINKVGEQEDHPGQTLATRNHDVIKHWAEERDGTPATVPGTQHDGRPGVLRFNFP
                     GYGGKDLKEVSWQDWFKTFDDRKLTFLFQQKLKNGHQSNFFMVDSPFREHD"
     gene            1162..1683
                     /locus_tag="VFU72_15595"
     CDS             1162..1683
                     /locus_tag="VFU72_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5424966.1"
                     /translation="MGAIVAVEELSWLGEQLRRQRKRVVLTNGHFDLRHVGHLHGLQA
                     ARRLGDVLVVGVNDDAGTTLRKGPTRPILPEEERAELLAALACVDYVVVFSDATAERL
                     VWLLRPDIYVKGGDYALTEDELVEGKAPLPEAEVVRGYGGEVRTIPLLPGRSTSAIER
                     AIVERAARETSGA"
     gene            1680..3347
                     /gene="murJ"
                     /locus_tag="VFU72_15600"
     CDS             1680..3347
                     /gene="murJ"
                     /locus_tag="VFU72_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU5424967.1"
                     /translation="MSLREAGAEAGTESDAVVEVAAATEPTRASSDRERIVKAAAIIM
                     LGTLASRVLGLGREQISSYLFGTGDAVAAFTIADNIHTMLFDLVMSGMMEAALVPVLS
                     AYTAPEQREELRRITGALLVLVLIIIGGAVLLLELFAPVAVRVMTAFGGGASARGADT
                     VALTVQIVRIILPAVLLLAISAVIMSTLYSLHRFTRPALSLAARNAAIIFAAVTLGRT
                     ALGVRSLAVGVVLGGLCLVLIQAPGLRDALPRPNFNFRHPAIKRIYTLYLPITVGLIS
                     NTIALIVDRNLAWGAGVNALGAMRYATALNQMILGLVATATSIASLPTLSRHFASGDE
                     EGYQRTLSNGLKMVTIMVVPATLGMAAIAWPAVRLLFEHGATSNAGARAIMTALLAYL
                     PGTFFAAFDQVLIFAFYARHNTRTPVTVGVLAVGVYFITALSLVHPLEMTGLVLANSA
                     QFTFHAIVMWLLLRRALGQVGDVTVGRTLRRALAAGGVMALGVLALSQVLRMLFESSG
                     SPVTLQFVAVIVPIGFGMLLYGGLLRALGVEEMRALQRGLLTRLGRA"
     gene            3351..3875
                     /locus_tag="VFU72_15605"
     CDS             3351..3875
                     /locus_tag="VFU72_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEU5424968.1"
                     /translation="MVLERIFSLWALARWLHLLAAITWIGGQLFILIVLLPIMRGALP
                     RDERVLLFARVGRRYGLVSWVALSVLIVTGVINGQRRGVNWAHLFQAGSYGQILGAKL
                     VLVAVVIVVTIVHAGYFGRRMTTLAERARALGSDDPALAAERRRLQVISGVLSGLNLL
                     LNLVIVLLAAALVA"
     gene            complement(4118..4330)
                     /locus_tag="VFU72_15610"
                     /pseudo
     CDS             complement(4118..4330)
                     /locus_tag="VFU72_15610"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
BASE COUNT          567 a         1473 c         1595 g          735 t
ORIGIN      
        1 acccgcgagc cctggctggc gatcgatccc aagggcgtgg tcggcgagcc ggccgccgag
       61 gtcggccctc tgctgctcaa cccccggccg ctgctcctct cctggccgca ccccgagcgc
      121 gtgctggcgc gacggctcga catattgtcc gaggcgctgg gcatcgagcg cgagcggctg
      181 tgggcctggg ggctggccca tgccatgctg tcggcctgct ggagcgtcga ggatttcggg
      241 gccggctggg agtacgccat cgcaatggcc gagctgctgg cggggatgcg ggggtagggc
      301 ggcggctgac gagcaggtgg gtgccgggta acgacggatc cttcgactcc gcgcagagcc
      361 tgccctgagc ttgccgaagg gatgacaggg ggaggggatg aggcgtacga gtacgtcacg
      421 tcgtattcgt cagccccgac ttaactctgt catcccgacc gcagtggagg gatccgtcct
      481 cgttggcacg gcctgtagtc gctgggcggc gcccctctcc cgatgctcgg gagaggggat
      541 ggggatgagg gcccgtctag tcgtgctcgc ggaaggggct atcgaccatg aagaagttgc
      601 tctggtggcc gttcttcagc ttctgctgga agaggaaggt cagcttgcgg tcgtcgaagg
      661 tcttgaacca gtcctgccaa ctgacctcct tgagatcctt gccgccgtag ccggggaagt
      721 tgaagcgcag cacgccgggg cggccgtcgt gctgggtgcc gggtacggtg gcgggcgtgc
      781 cgtcgcgctc ctcggcccag tgcttgatca cgtcatggtt gcgggtggcc agcgtctgtc
      841 cggggtggtc ctcctgctcg cccaccttgt tgatccatct ggcggtcagc gtggtgcgcg
      901 agagcttgtc ggcgtactgc tcgagatacg ccttgtcttc cttggtcagg ttctcggcac
      961 ggtcgatcag gtacttgttg ccgctctcgt gctgctgctg gtgctgttgc tccgacatgc
     1021 tgtcctccaa tgcgttggga acacgaaatc agccccgcgc gctcggcgca ctccgcgtgc
     1081 cattgtcccg agcctgatgc ggcggtggcc ggcgtgctac cattcggtgt tttcgccggg
     1141 gggcacgctg gaagggatgc gatgggcgcg atcgtcgcgg tcgaggagct gagctggctg
     1201 ggcgagcaac tgcggcgcca gcgcaagcgc gtggtcctga ccaacgggca tttcgacctg
     1261 cggcacgtcg gccacctgca cggcttgcag gcggcgcggc ggctgggcga cgtgctggta
     1321 gtgggcgtca acgacgacgc cggcaccacg ctgcgcaagg ggccgacccg gccgatcctg
     1381 cctgaggagg agcgtgccga gctgctggcg gcgctggcct gcgtcgatta cgtggtggtc
     1441 ttctccgacg ccaccgccga gcgcctcgtc tggctgctgc ggccggacat ctacgtcaag
     1501 ggcggcgact acgcgttgac cgaggacgag ctggtggagg ggaaggcgcc gctgccggag
     1561 gccgaggtcg tgcgcggcta cggcggcgag gtgcgcacca tcccgctctt gccagggcgc
     1621 tcgaccagcg ccatcgagcg cgccatcgtg gagcgggcgg cgcgcgagac gagcggggca
     1681 tgagcctgcg cgaggcgggg gcagaggcgg gcaccgaaag cgacgccgtc gtggaggtgg
     1741 ccgcggccac ggagcccacc cgcgcctctt ccgaccgcga gcggatcgtc aaggccgccg
     1801 cgatcatcat gctcggcacc cttgccagcc gcgtgctcgg cctgggccgc gagcagatct
     1861 cctcctacct cttcggcacg ggcgacgcgg tcgccgcctt caccatcgcc gacaacatcc
     1921 acaccatgct cttcgacctg gtgatgagcg ggatgatgga ggccgcgctg gtaccggtgc
     1981 tgagtgccta caccgcgccg gagcagcgcg aggagctgcg tcgcatcacc ggcgcgctgc
     2041 tggtgctggt gctgatcatc atcggcggcg cggtgctgct gctggagcta ttcgcgccgg
     2101 tggcggtgcg ggtgatgacc gcgttcggcg gtggggccag cgcgcgcggc gccgatacgg
     2161 tcgcgctgac cgtgcagatc gtgcgcatca ttctgccggc ggtgctgctg ctggcgatct
     2221 cggccgtgat catgtcgacg ctgtactcgc tgcaccgctt cacccgcccg gcgctctcgc
     2281 tggcggcgcg caacgcggcc atcatcttcg cggcggtcac cttggggcgc acggcgctgg
     2341 gggtgcgcag cctggcggtc ggtgtggtgc tgggcgggct ctgcctggtg ctgatccagg
     2401 caccggggct gcgcgacgcc ctgccgcggc ctaacttcaa cttccgccac ccggcgatca
     2461 agcgcatcta cacgctctac ctcccgatca ccgtcggact gatcagcaac acgatcgcct
     2521 tgatcgtgga ccgcaacctg gcctggggcg ccggggtcaa cgcgctgggc gcgatgcgct
     2581 acgccaccgc gctgaaccag atgatcctgg gactggtggc caccgccacc tcaatcgcct
     2641 cgctgccgac gctctcgcgc catttcgcca gcggcgacga ggagggctat cagcgcaccc
     2701 tctccaacgg gctgaagatg gtcaccatca tggtcgtgcc ggccacgctg ggcatggcgg
     2761 ccatcgcctg gccggcggtg cgtctcctct tcgagcatgg cgcgaccagc aacgccgggg
     2821 cgcgcgcgat catgaccgcc ctcctggcct acctgcccgg caccttcttc gccgccttcg
     2881 atcaggtgct gatcttcgcc ttctacgccc gccacaacac gcgcacgccg gtcacggtcg
     2941 gcgtgctggc ggtcggcgtc tacttcatca ccgcgctctc gctggtgcac ccgctggaga
     3001 tgacggggct ggtgctggcc aactcggcgc agttcacctt ccacgccatc gtgatgtggc
     3061 tgctcttgcg ccgggcgctg gggcaggttg gcgacgtcac cgtcgggcgc acgctgcggc
     3121 gggcgctggc cgccggcggg gtgatggcgc tgggggtgct ggcgctctcc caggtgctgc
     3181 gcatgctgtt cgagtcgagc ggctcgccgg tcacgctgca gttcgtcgca gtaatcgtgc
     3241 cgatcggctt cggcatgctg ctctacggcg ggctgctgcg ggcgctgggg gttgaggaga
     3301 tgcgcgccct ccaacgcggc ctgctgacgc ggctgggacg ggcgtagcgc gtggtgctgg
     3361 agcgcatctt ctcgctctgg gcgctggccc gctggctgca cctgctggcg gccatcacct
     3421 ggatcggcgg ccagctcttc atcctgatcg tgctgctgcc gatcatgcgc ggcgcgctgc
     3481 cgcgcgacga gcgcgtgctc ctcttcgcgc gcgtggggcg gcgctacggc ctggtctcct
     3541 gggtggcact ctcagtcttg atcgtaaccg gggtgatcaa cggccagcgg cgcggggtca
     3601 actgggcgca cctcttccag gccggcagct acgggcagat cctgggcgcc aaactggtgc
     3661 tggtggcggt ggtgatcgtg gtcacgatcg ttcacgcggg ctacttcggg cggcgcatga
     3721 cgaccctggc tgagcgcgcc cgcgccctcg gcagcgacga cccggcgctg gccgctgagc
     3781 gccggcgcct gcaggtgatc tccggcgtcc tctcgggtct caacctgctg ctcaacctgg
     3841 tgatcgtgct gctggcggcc gcgctggtgg cctgaggcgg gggtgccggt ggacgacggg
     3901 gctcggcgtg cgtcggcccg cgcacgggcc ggcgatgccg cgcgcgcagg ccggccaggt
     3961 cgctgggccg tgccagcgcc aggatggcga ccgcggcggc gacgaccgcc atgctgagcc
     4021 ccatcgccag cgagccgcgg ccggcctcgg aggcggtgaa gacgttgaac agaccgtgcc
     4081 agaggatgac catcagcagg ctgccgctgc cgttcgcgag ccaggtgagc aggacggccc
     4141 cggccagcag gctgaaggcc agctccggca cgccaaccag ccccatctcc cggtagctcg
     4201 gcacatagaa gaagaagggc gcgtgccagc ccagccagac cagccagagc aggagcgtgg
     4261 ccttcagcgc gccgcgccgc cgctgcagcg cgggcaacag atagccgcgc cagccgacct
     4321 cctcgcccaa tccgctggtc aggatccaga gcgggatggc cgccagcccc
//
LOCUS       DASUBI010000796         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_941157, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000796 DASUBI010000000
VERSION     DASUBI010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941157"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..292)
                     /locus_tag="VFU72_15615"
     CDS             complement(2..292)
                     /locus_tag="VFU72_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5424969.1"
                     /translation="MRKSDLIKDVSQKVQQPESQVGAVVNATFSAIQDALSEGQDVSI
                     AGFGTFRISERSAREGRNPQTGQRITIPARKSPGFKPGTQLKRAVAGESEEA"
     gene            complement(416..1216)
                     /locus_tag="VFU72_15620"
     CDS             complement(416..1216)
                     /locus_tag="VFU72_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5424970.1"
                     /translation="MSSLPALTSQHNPTVKYARSLHQRKIRYRERALLVEGVRLYLDA
                     IAGGARPTLVFLDEERLGREAERVRAAAEGARAYSVTPTVLDAIDDTRTPQGVVAVFP
                     FPEPPLELTPGSAPLFIVSDGLQDPGNLGTLTRSALGAGANALFITTATVDPFAPKVV
                     RAGMGAQFRLPIRWLDWNEPDPLILACEQLLAARADAPGIYTQIDWTRPSSIVIGNEV
                     RGISPRAGALLTGSIAVPLVGGLESLNAGVAGSIILFEAARQRRATRA"
     gene            complement(1226..1579)
                     /gene="rplT"
                     /locus_tag="VFU72_15625"
     CDS             complement(1226..1579)
                     /gene="rplT"
                     /locus_tag="VFU72_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5424971.1"
                     /translation="MVRVKRGVAAHRRHVNKLKMARGYRGTRSRLYRRANEAVMKALS
                     YQYRDRRRRKRDFRRLWITRINAAARQNGMPYGRFIQGLTKAGVEVDRKMLADLAVRE
                     PEAFARFVELAKTQL"
     gene            complement(1601..1798)
                     /gene="rpmI"
                     /locus_tag="VFU72_15630"
     CDS             complement(1601..1798)
                     /gene="rpmI"
                     /locus_tag="VFU72_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5424972.1"
                     /translation="MPKMKTNKGAKRRFKVTGTGKVLMMKGLRSHNRRKKSKHSLRSM
                     DKMFEVPSSYKKQLKKLLPYR"
     gene            complement(1825..2451)
                     /gene="infC"
                     /locus_tag="VFU72_15635"
     CDS             complement(1825..2451)
                     /gene="infC"
                     /locus_tag="VFU72_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5424973.1"
                     /translation="MGRASRSRRDGDAAAQYVLQEASISTGRPIRINERIRIPEIRLI
                     DENGTHIGVLRTRDALEMARERGLDLVEVQPNAVPPVCRLMDYGKFRYEESRRERESR
                     KRQKTADVKEIRLSPKIGEHDLETKGRLALKFLEAGDKVKLTVRFRGREIAHQDIGRG
                     ILERMTQELGAAAAVDQVPHIEGRTMVTLLSPTKGARGSEEHQQAAEA"
     gene            complement(2492..>3410)
                     /locus_tag="VFU72_15640"
     CDS             complement(2492..>3410)
                     /locus_tag="VFU72_15640"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEU5424974.1"
                     /translation="VAVAVGVVVALALVARFPALLLFGVPLYFAAWLWFNRSLDLARR
                     FLVGVLALAYSLLLVTEFVYLQDAFQNRMNTLFKLYFQVWALFAVCAAVALPLSLRWL
                     RQRAGRPAEFTLAVILAVLILGAAVYPPLSAYRWTDGLHHFQGIDGLAYLRQVAPDDA
                     AGIAWLQQHAQTGQRVLEAAGCSYADRSGEPADRVSMATGLPTIVGWDFHEFQWRQGL
                     PDAQAQINARQAAVKQIYDAPTSRSARALLDKYGIDYIYVGDFEREGYPNQCAAGPPY
                     SAQGLASLSQLGWPVAFQQGSVTIYARPS"
BASE COUNT          568 a         1082 c         1154 g          606 t
ORIGIN      
        1 attaagcttc ttctgactcg ccggccacgg cgcgcttgag ctgcgtgccg ggcttgaagc
       61 cggggctctt tcgcgcgggg atggtgatgc gctgcccggt ctgcgggttc cgtccttcgc
      121 gcgcgctgcg ctcactgatg cggaaggtgc cgaaaccggc gatcgagaca tcctgaccct
      181 ctgacagggc gtcctgaatc gcgctgaagg tggcattgac cacggcgccg acctggctct
      241 ccggctgttg caccttctgc gagacgtctt tgatcaggtc gctcttcctc atgaatatcc
      301 tccatctccg cccatgcttg ccagcaagcc ttgagcatgt gcggtcgcct acgcacacgg
      361 cacgccaccg tgacgcgata ttgcgcgtat gtatgcccta ttccgctgcg cggcgtcaag
      421 cccgggtcgc tcggcgctgg cgtgcagctt cgaaaagaat gatggaaccg gcgacgccgg
      481 cgttgagtga ctcgagtccg ccgaccaacg ggaccgcgat gctgccggtg aggagcgcgc
      541 cggcccgcgg cgagataccg cgcacttcgt tgccgatgac gatagatgag gggcgtgtcc
      601 agtcaatctg cgtatagatc cccggtgcgt ccgcacgagc ggccagaagt tgttcgcatg
      661 cgagaataag cggatccggc tcgttccagt cgagccagcg gatcggcaag cgaaactgcg
      721 cacccatgcc ggcccgcacg accttggggg cgaaggggtc gacggtggcg gtggtgatga
      781 agagcgcgtt ggcgcccgcg cccagcgccg agcgcgttag tgtgccgaga ttgccggggt
      841 cttggaggcc atcactgaca atgaagagcg gcgcgctgcc gggcgtcagc tcgagcggcg
      901 gctcggggaa cgggaagacc gccacgacgc cctgcggggt gcgtgtgtcg tcgatggcgt
      961 cgagcacagt gggggttacg gagtaggctc gcgcgccctc ggctgccgcc cgcacgcgct
     1021 cggcctcccg accgagccgc tcctcatcca gaaagacgag cgttggccgc gccccgccgg
     1081 cgatcgcgtc gaggtagagg cggacgccct cgacaagcaa cgcccgctcg cgatagcgga
     1141 tcttccgctg atgcagcgag cgggcgtact tgaccgttgg gttgtgttgg cttgtcagcg
     1201 caggcagcga cgacatggcg ccgggctaga gctgcgtctt ggccagctcc acgaaccgcg
     1261 cgaaggcttc cggctcacgc accgcgagat cggccagcat cttgcgatcg acttcgaccc
     1321 ccgccttggt caaaccctgg ataaagcggc cgtagggcat gccgttctgg cgcgcggccg
     1381 cgttgatgcg ggtgatccag aggcgccgga agtcacgctt gcgccgacgg cggtcacggt
     1441 actgatatga gagcgccttc atgaccgctt cgttggcgcg tcggtagagg cggctcctgg
     1501 ttccccggta gccgcgagcc atcttcagct tgttgacgtg acgacggtgg gccgccacgc
     1561 cgcgcttgac gcgaaccatg tctcgtccct gctccctgaa ctaccggtag ggaagcagct
     1621 tcttgagctg cttcttgtac gacgacggca cctcgaacat cttgtccatc gagcgcagcg
     1681 agtgcttcga cttcttgcgc cggttgtggc tgcgcaggcc cttcatcatc agcaccttgc
     1741 cggtgccggt gactttgaag cggcgcttgg cgcccttgtt cgttttcatc ttcggcatcg
     1801 cgtgctcacc cttcgaaatg tcccttaggc ctcggcggcc tgctggtgtt cctcactgcc
     1861 gcgagcgccc ttcgtcgggc tcagtagcgt gaccatggtt cggccttcaa tgtgcggcac
     1921 ctgatccacc gcggcggccg cgcccagttc ctgagtcatg cgctccagga tgccccgtcc
     1981 gatatcctgg tgcgcgatct cccggccgcg aaaccgcacg gtgagtttga ccttgtcgcc
     2041 cgcttcgagg aacttgagtg ccagacggcc cttggtctcg agatcatgct cgccgatctt
     2101 cgggctgaga cgaatctcct tgacgtcggc cgtcttctga cgcttgcgcg actcgcgctc
     2161 gcgccggctc tcctcatagc ggaacttgcc ataatccatc aggcgacaca ccggcggaac
     2221 cgcgttgggc tgtacttcga cgaggtcgag cccgcgctcg cgcgccatct caagcgcgtc
     2281 tcgcgtgcgc aagacgccga tgtgggtgcc gttctcgtcg atcaagcgga tctctgggat
     2341 gcggatacgc tcgttgatac ggatgggtcg tccggtgctg atggatgcct cctgtaaaac
     2401 atactgagcc gcagcatcgc cgtcgcgtct gctgcggctg gctcgtccta tgaccgatca
     2461 atgatagcac aggcaaaatc gcggggtcaa ttcaacttgg gcgcgcgtag atcgtcaccg
     2521 aaccctgctg aaaagcgacc ggccagccga gctgggacag cgacgcgaga ccctgggcgg
     2581 agtagggcgg tcccgccgcg cactggttgg ggtagccctc gcgctcgaaa tcgcccacgt
     2641 agatgtagtc gatgccgtac ttgtccagca gcgcgcgcgc gctgcgtgag gtgggagcgt
     2701 cgtagatctg cttgacggcg gcctggcgcg cgttgatctg ggcctgggcg tcgggcagcc
     2761 cctggcgcca ttgaaactcg tggaaatccc aaccgacgat ggtgggcagg ccggtcgcca
     2821 tcgagacgcg atcggccggt tctccggagc ggtcggcgta ggagcagccg gccgcttcca
     2881 gcacgcgctg gcctgtttgg gcatgctgct gcagccaggc gatgccggcg gcgtcgtctg
     2941 gtgccacctg gcgtaggtag gcaagtccat cgatcccctg gaagtggtgc aacccgtcgg
     3001 tccagcggta cgcagagagc ggtgggtaga ccgcggctcc gaggatcagc acggcgagga
     3061 tgaccgcgag cgtgaactcg gccgggcgac ccgcgcgctg ccgcagccag cggagcgaca
     3121 gcggcagcgc gaccgccgca cagaccgcga agagcgccca gacctggaaa tagagcttga
     3181 agagggtgtt catgcggttt tgaaaggcgt cctgcaggta gacgaattcg gtgacaagaa
     3241 gcagcgaata ggcgagggcg aggacaccca ctagaaaacg ccgggcgagg tcaagcgagc
     3301 ggttaaacca gagccaggcg gcgaagtaga gcggcacgcc gaagaggagc agcgcgggga
     3361 agcgcgcgac aagcgccagg gctacaacca cgccgacggc gaccgcgacg
//
LOCUS       DASUBI010000797         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_393830, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000797 DASUBI010000000
VERSION     DASUBI010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393830"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFU72_15645"
     CDS             complement(<1..468)
                     /locus_tag="VFU72_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEU5424975.1"
                     /translation="MAASEPAELLLAQIWHAQWLTDALALGDGRPLRVVYRGVWTHGL
                     GPDFRGAYLDLGGALREGDVEVHRRASDWVRHGHHLDPAYNTTILHTVLDDDLGQPVR
                     RQDGAAVSTLCLRDALPGPLDAFPSDASLRPLGAIGFAHCAPQVAASDPGALVA"
     gene            complement(481..1347)
                     /locus_tag="VFU72_15650"
     CDS             complement(481..1347)
                     /locus_tag="VFU72_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5424976.1"
                     /translation="MALARVNGVDIFYEEAGAGPPVVFSHEFAGDAQSWAPQVHAFSR
                     LYRCLTYNHRGFPPSSVPDDPAAYSQDLLVEDLRGLLDTLGIERAHLVGLSMGGNVVL
                     NFALRYPERCHGVVVAGCGAGTTNRERFERDIEQIVDILATRGMAAFAETYSQGATRL
                     PFRRKDPAGWQVFRERLAEHSARGMMLTQLGVQRLRPTVYQLEEHLRQLRVPALILIG
                     DEDEPCVDPAVFMKRVIPSAGLVVIPQSGHTINLEEPARFNDAVLDFLRLVEAGRWPV
                     RQQVTTSLLPPG"
     gene            1477..2172
                     /locus_tag="VFU72_15655"
     CDS             1477..2172
                     /locus_tag="VFU72_15655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5424977.1"
                     /translation="MNFRAVLFDWDDTLAAYRPHRYETVEAVLHAHGVPIDRRQLHRA
                     WSVADDPTVAGRDAGFWERFGRELSLEDPRVLAALDEAFARRDLVKRLTIFEDALALL
                     EHLTARAIPAGILSNNVEAGRFADELGVLARFRAVVTPREAGAGKPEPAIFHYALERL
                     GTRPEETLYVGDTYEHDVLGARAAGLPVVLVDRLGLCDATDCTRLESLAGVVADAGFV
                     ASPVTGIARCHPE"
BASE COUNT          310 a          769 c          809 g          308 t
ORIGIN      
        1 cgccaccagc gcgcccgggt cggacgcggc cacctgcggc gcgcagtggg cgaagccgat
       61 cgcgcctagg gggcgcaggc tggcgtcgct ggggaaggca tcgagcggac ccggcagcgc
      121 gtcgcgcagg cagagcgtgg acaccgcggc gccgtcctgc cggcgcaccg gctggcccag
      181 atcgtcgtcg agcacggtgt gcaggatggt cgtgttgtag gccgggtcaa ggtggtgacc
      241 gtggcggacc cagtccgagg cgcggcggtg gacctcgacg tccccttcgc gcagcgcgcc
      301 gccgagatcg agataggcgc cgcggaaatc cggccccagc ccgtgcgtcc agacgccacg
      361 gtagacgact cgcagcggtc gcccgtcgcc gagcgccagc gcatccgtca gccactgcgc
      421 gtgccagatc tgcgccagca gcagctcggc cggctcggag gcggccacgc gcggggcctg
      481 ctagcccggt ggcagcaggc ttgtcgtcac ctgctggcgc accggccagc ggccggcctc
      541 gaccaggcgg aggaagtcga gcaccgcgtc gttgaagcgc gccggctcct ccaggttgat
      601 ggtgtggccg ctctggggga tcaccaccag cccagccgag gggatgacgc gcttcatgaa
      661 gacggcagga tcgacgcagg gctcgtcctc gtcgccgatc aagatcaacg ccggcacgcg
      721 cagttggcgc aggtgctctt cgagctggta gaccgtcggg cgcaggcgct gcacgccgag
      781 ctgggtgagc atcatgccgc gcgccgagtg ctcggccagc cgctcccgga agacctgcca
      841 gccggcgggg tccttgcgcc ggaaaggaag ccgggtggcg ccctggctgt aggtctcggc
      901 gaaggccgcc atgccgcgcg tcgccaggat gtcgacgatc tgctcgatgt cccgctcgaa
      961 gcgttcgcgg ttggtggtgc cggcgccgca gccggccacc acgacgccgt ggcagcgctc
     1021 ggggtagcgc agcgcgaagt tgagcaccac gttcccgccc atcgagagtc cgaccaggtg
     1081 cgcgcgctcg atcccgaggg tgtcgagcag cccgcgcagg tcttcgacta gcaggtcttg
     1141 cgagtaggcg gccgggtcgt cgggcacgct ggagggcggg aagccgcggt ggttgtaggt
     1201 caggcagcgg tagaggcgcg agaaggcgtg cacctggggc gcccagctct gcgcgtcccc
     1261 ggcaaattcg tggctgaaga cgaccggcgg ccccgcgccg gcctcctcgt agaagatatc
     1321 gaccccgttc acccgcgcca gcgccatgct cacccccgct ccgcctatcc ccctggattg
     1381 cagcgcatgg tagccgaggc ggcggcacgc gtctagccgg cgttccgtcc gtacgcgtaa
     1441 gataggcggg tgaaccggcc tggcaacgga ggaccgatga atttccgcgc cgtgctcttc
     1501 gactgggacg acacgctggc cgcctaccgt ccgcaccgct acgagacggt cgaggcggtg
     1561 ctccacgcgc acggcgtgcc catcgaccgc cgccagctgc accgcgcttg gtccgtggcc
     1621 gacgatccga ccgtcgccgg gcgcgatgcc ggcttctggg agcgcttcgg gcgtgagctg
     1681 tcgctggagg acccgcgggt cctggccgcg ctcgacgagg ccttcgcccg gcgcgacctc
     1741 gtcaagcgct tgaccatctt cgaggacgcg ctcgcgttgc tggagcacct gacagcgcgc
     1801 gccatcccgg ccggcatcct ctcgaacaac gtcgaggcgg ggcgctttgc cgacgagctc
     1861 ggcgtgctgg cgcgcttccg agcggtagtg actcccaggg aggcgggcgc gggcaagccg
     1921 gagccggcca tcttccacta cgccctggag cggttgggca cccgcccgga ggagaccctc
     1981 tacgtcggcg atacctacga gcacgacgtg ctcggcgccc gcgccgccgg tctgccggtc
     2041 gtcctggtcg atcgcctcgg cctctgcgac gcgaccgact gcacacggct ggagtcgctc
     2101 gcgggcgtgg tggcggatgc gggcttcgtg gcgagcccag tgacagggat cgcgcggtgc
     2161 catccggaat aaaatcgcca ctacccctgt gggggg
//
LOCUS       DASUBI010000798         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1001550, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000798 DASUBI010000000
VERSION     DASUBI010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001550"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..728
                     /gene="scpB"
                     /locus_tag="VFU72_15660"
     CDS             147..728
                     /gene="scpB"
                     /locus_tag="VFU72_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5424978.1"
                     /translation="MPEPAQQLPLEAIAPEERVAALGALLFAAPEPVPLPLLAQQLGC
                     TPDEARAAAATLAGRLDEVGLLLQWVDEALQLASAPRFARLLQRFLGLERSVRLSQAA
                     LETLAIIAYRQPVTRLGIEAVRGVDSTGVLQTLLARGLVEPVGRLAAVGSPVLYGTAP
                     EFLRFFGLTSLAELPPLPEELAALTERAAEHPG"
     gene            complement(779..1285)
                     /locus_tag="VFU72_15665"
     CDS             complement(779..1285)
                     /locus_tag="VFU72_15665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5424979.1"
                     /translation="MAGRVADVRSRLRDESGSRSADAILIRQARLADLWSLWQLQRRC
                     FEPGQAYGLPSLTVLYAWPRALILVACSDERQAGCIVADLHERQGRILNLCVDPDFRR
                     RGIASTLMTLAEEILPTDHLALMVEDKNHGAQALYRKLGYLPVGDLRNYYGRNRHGIL
                     MEKRREAV"
     gene            complement(1340..1636)
                     /locus_tag="VFU72_15670"
     CDS             complement(1340..1636)
                     /locus_tag="VFU72_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424980.1"
                     /translation="MDNFANGIVLLGVPAVVLVPLVVEGLKRLGLPTRWATPAAMLTG
                     GLVAALAQAVDAWPRLDPLVRIVLAAVVFGFAASGAYSQAAQLRPGGAASEPPE"
     gene            complement(1659..>2237)
                     /locus_tag="VFU72_15675"
     CDS             complement(1659..>2237)
                     /locus_tag="VFU72_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424981.1"
                     /translation="WTVANQAVDVAAINCELEGFARDGATEAQYRALAAVFRWCRTVG
                     MTVPAEYVGRDERPGIIGHMDVAAPNGDGWGGASHHTDPGPLFTWDHLVDLIGGGAAA
                     GVRRDPVTGCTIAGGFRAYYEWLEERLGVDDALLDVGRPISDELVEDGLTVQYFERAV
                     FERHEGARPARWDVLRRRLGADVLSARRARWS"
BASE COUNT          323 a          856 c          748 g          310 t
ORIGIN      
        1 gcgcttctcc gagctgctcg gcccggccgc cagccgcgcc gagcaggcgg tggccttcat
       61 cgcgctgctg gcgctgctgc gtcgccgcgc ggtcgaggcg cgccaggccg cgctcttcgg
      121 cgagatcgac gtgatcccgt acaccgatgc ctgagccggc ccaacagctc ccgctcgaag
      181 ccatcgcgcc cgaagagcgc gtcgccgcgc tcggcgcgct cctcttcgcc gcgccggagc
      241 cggtgccgct gccgctgctg gcgcagcagc tcggctgcac gccggatgag gcgcgcgccg
      301 cggcagccac gctggccggg cggttggacg aagtcggctt gctgctgcag tgggttgatg
      361 aggcgctcca gctcgccagc gcgccccgct tcgcccggct gctgcagcgc ttcctcggac
      421 tggagcgcag cgtgcgcctc tcgcaggccg cgctggagac actggccatc atcgcctacc
      481 gccagccggt gacgcggctg gggatcgagg ccgtgcgcgg ggtcgattcc accggcgtgc
      541 tgcagacgct gctggcccgc gggctggtcg agccggtggg gcggctggca gccgtcggca
      601 gcccggtcct ctacggcacc gctcccgagt tcctacgctt tttcggactg accagcctgg
      661 cggagctacc cccgcttccc gaggaactgg ccgcgctgac cgagcgggcc gctgagcacc
      721 caggttgagg cggcagtcga ggtaacggcg cgccgcgtaa ggtgaatagg cagtgcccct
      781 agaccgcctc ccgccgcttc tccatcagga tgccgtggcg gttgcggccg tagtagttgc
      841 gcaggtcgcc gaccgggagg tagccgagct tgcggtagag cgcctgcgct ccgtggttct
      901 tgtcctccac catcagggcc agatggtcgg tcgggaggat ctcctcggcc agggtcatca
      961 gcgtgctggc gatgccgcgc cggcgaaaat caggatcgac gcagaggttg aggatgcggc
     1021 cctgccgctc gtgtagatcg gccacgatgc agcccgcctg acgttcgtct gagcaggcga
     1081 ccagaatcag cgcgcgcggc caggcgtaga gcacggtgag cgacggcaag ccgtaggctt
     1141 ggcccggctc gaagcagcgg cgctgcagct gccagagcga ccagagatcg gccaggcgcg
     1201 cctggcgaat aaggatcgca tccgccgacc tgctgcccga ctcgtcgcgg agtctgctcc
     1261 gaacgtcagc cacgcggccc gccatgcgtc ctcctcgacc tgggagcggg caggcgctcg
     1321 ctcgtctgct gagtatagcc tactcgggag gctcgctcgc cgcgccgccc ggccgcagct
     1381 gcgcggcctg cgagtaggct ccgctggcgg caaagccgaa gacgaccgcc gcgagcacga
     1441 tccggaccag tgggtcgagc cgtggccaag catcgaccgc ctgggccagc gccgcgacca
     1501 ggccgccggt cagcatggcg gctggcgtag cccagcgcgt cggcaagccg agccgcttca
     1561 ggccctccac caccagcggc accagcacga cggccggcac gcccagcagc acgattccat
     1621 tggcgaagtt gtccacgcgc gtccctccac cagtcggctc acgaccaccg cgctcgccgg
     1681 gcggagagca cgtccgcccc cagccggcgc cgcaacacgt cccagcgcgc cggccgggcc
     1741 ccctcatgcc gctcgaagac cgcccgctcg aagtactgca ccgttagccc atcctccacc
     1801 agctcgtccg agatcggccg cccaacatcg agcaaggcgt cgtccacccc cagccgttcc
     1861 tccagccact cgtaataggc gcggaaccca ccggcgatcg tgcagccggt caccgggtct
     1921 cgccgcacgc cagcagccgc gccgccgccg atcaaatcca ccaggtgatc ccacgtgaag
     1981 agcggccccg ggtcggtatg gtggctcgcc ccgccccagc catccccgtt tggtgcggcc
     2041 acgtccatgt gcccgatgat cccgggccgc tcatcgcgcc cgacatactc ggccggcacc
     2101 gtcataccga cagtccggca ccagcggaac accgccgcca gcgcgcgata ctgcgcctcg
     2161 gtcgcgccgt cccgcgcgaa gccctccagc tcgcagttga tcgccgccac atcgaccgcc
     2221 tgattggcca ccgtcca
//
LOCUS       DASUBI010000799         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_457114, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000799 DASUBI010000000
VERSION     DASUBI010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457114"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(482..1675)
                     /locus_tag="VFU72_15680"
     CDS             complement(482..1675)
                     /locus_tag="VFU72_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5424982.1"
                     /translation="MPRHPLNDKRLLTAVLLGAAVAAWAGNRTRESLLEHTKPQTSTL
                     IDWEQARSLAHGMNRGSVIDAEQRQALTEYYFNLTQRTIPIVAEYTGDTMLPTLEQVY
                     AFDRNDWIDANILGFKEMFRPLEALNPMGEEESVVANLLWGGLSQKVLSVELGFLLGY
                     LARRVLGQYDMTLLGREPVSAGKLYFVQPNIAHVEQTLDLPREDFRLWLALHETTHAF
                     EFEAHPWLRGHVNSLLESYFEFLNQDMETLKRGLPGLKVFWERARGTEREPGQWIQAV
                     MTPEQRELFNAMQATMSVIEGYSNHVMNAVGQRLIPTYEVISKRFERRQRQRTPAERL
                     FARLTGLDIKMEQYRQGEAFINAVVQQRGHDFAKLVWRGPEFLPSMEELRNPDQWIAR
                     VSALA"
     gene            complement(1752..>3649)
                     /locus_tag="VFU72_15685"
     CDS             complement(1752..>3649)
                     /locus_tag="VFU72_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEU5424983.1"
                     /translation="QLNVRYDAEQTAAQAFALGHDGNLWSDSADLLSAWLGGSRVPVV
                     FSVDQQASLDALRTLAPEVARPPVDATYVFDTGGKLTIDPGSTGVGINTAATYGAIER
                     SVAALSSEPVAIQTVGLPQQVEAKDLQPGLAQASALISQPVSIRFGGDRWEIAPATLR
                     TMLARADTGQAAPTLSRQELATYLGQLDGAIKRTGVDAGVKWDGKAFAVVSGQDAQAF
                     DPAATADAILKALGQGQHAVDAVTQAHAPAITDADARAAADQAQQLVAKPFTVTWDGG
                     SLPLPAEVIGQSVSFQAHPGDSQKLTMSLDPAALTQALDGIKDKIEKPAKDAQFRYLD
                     GAVKVVAPEQAGVTLDLQKSSEAVRDALLKGSTSAAIVTKPVEPKYTAAMASSIVISD
                     KLGSGQTSYAGSIANRAYNVELAVERVNGALVPPGGTFSFTGTIGAVDLKNGYKLGYG
                     IATTNGSVTTVPSVGGGICQVATTLFHAAWWAGMPIVDRSWHLYWIPNYGVAPDGVTG
                     LDATVDTDAGLDFKFKNTSGNWIAVVASADGSWVRFSLWGVDPHWKVESADPVITNVV
                     KADTTMKYEQGDSLPKGESILLEHAEDGFDVTLHRQVFSGDKLIDDLTLKSHYVPSEN
                     ITLVGTG"
BASE COUNT          570 a         1249 c         1182 g          648 t
ORIGIN      
        1 cggcctcgac ggtggggacg acaccctgca ggcgcgtcag gccctcggag aaagcggcgc
       61 ggtcgtcctg ctccaccatc ttgttgagat cgctgctgat cagtgacaga tcgagcgtca
      121 tgcgcgcccc cgctcgcggc cggtccggtc gaatctcggt cgccgagttc gtatcgaaat
      181 cggaacgaga tggattgcgg gccagacgtg gtgcctcccg agaaggtcgt gtgccgcgcc
      241 agcacggatc cttcgacaag ctcaggatga cagggtacgg gatgcggccc taccggacga
      301 gatgcatcct cgcctgatgg cgatcctcct cgtcagcccc tcatcaaccc tgtcttccag
      361 accgaagtgg agggatccgt cctcgctggc accccgcacg gctgtcccgc accgttctga
      421 acggtttgac tcctatcctg ccgaacgcac gccgccgcgc gaatcgtgac aacctgtccg
      481 atcaggccag cgcgctgacg cgggcgatcc actggtccgg gttgcgcaac tcctccatgc
      541 tcggcaggaa ctccgggccg cgccagacga gcttggcgaa gtcgtgcccg cgctgctgaa
      601 ccaccgcgtt gatgaacgcc tcgccctgac ggtattgctc catcttgatg tcgaggccgg
      661 tcagccgcgc gaagaggcgt tcggcgggcg tgcgctgccg ctggcgccgt tcgaagcgct
      721 tcgagatcac ctcgtaggtc ggaatcaagc gctggcccac cgcgttcatg acgtggttgc
      781 tgtagccttc gatcaccgac atcgtggcct gcatcgcgtt gaacagctcg cgctgctcgg
      841 gcgtcatcac cgcctgaatc cactggcccg gctcgcgctc ggtaccccgc gctcgttccc
      901 agaagacttt cagccccggg aggccgcgct tcaacgtctc catgtcctga ttgaggaact
      961 cgaagtacga ctccaagagc gaattgacgt gaccgcgcag ccagggatgc gcctcgaact
     1021 cgaaggcgtg ggtcgtctcg tgcagcgcca gccagaggcg aaagtcctcg cgcggcaagt
     1081 cgagcgtctg ctcgacgtgc gcgatgttgg gctgcacgaa gtagagcttg ccggccgaga
     1141 ccggctcgcg ccccagcagt gtcatgtcgt actggcccag tactcgccgt gccaggtagc
     1201 cgagcagaaa gcccagctcg acgctgagca ccttctgact cagcccgccc cagaggagat
     1261 tggcgacaac cgactcctct tcgcccatgg ggtttagcgc ctccaacggg cggaacatct
     1321 ctttgaatcc gaggatgttg gcatcgatcc aatcgttgcg gtcgaacgcg tagacctgct
     1381 ccagcgtggg cagcatcgtg tcgccggtgt actccgccac gatcgggatg gtccgctgcg
     1441 tcaggttgaa gtaatactcg gtcagcgcct gccgctgctc cgcgtcaatc acgctgccgc
     1501 ggttcatgcc gtgggccagc gagcgcgcct gctcccagtc gatcagagtc gatgtctgcg
     1561 gcttggtgtg ctccagaagc gactcgcgcg tgcggttgcc ggcccaggcg gccaccgccg
     1621 cgccgagcag gaccgccgtg agcaatcgct tgtcgttcag gggatgacgt ggcacgagca
     1681 gctcaccttt ccagcaaacg tggcgcgcct ggtcgcgcag agccgggacg gcgcgccgcc
     1741 gatgctcaag tctaccccgt cccgaccaac gtaatgttct ccgacggcac gtagtggctc
     1801 ttgagcgtca ggtcgtcgat cagcttgtcg ccgctgaaga cctggcggtg cagcgtgacg
     1861 tcaaacccgt cttccgcatg ctccagcaag atcgactcgc ccttcggcaa ggagtcgccc
     1921 tgctcgtatt tcatggtggt gtcggccttg acgacgttgg tgatgaccgg atcggccgac
     1981 tccaccttcc agtgcgggtc gacgccccac agcgagaagc gcacccaact gccgtcggcg
     2041 ctggccacca cggctatcca gttgccgctg gtgttcttga acttgaagtc gagccccgca
     2101 tcggtgtcga ccgtggcgtc cagcccggtg actccgtccg gagccacgcc atagttgggg
     2161 atccagtaga ggtgccaact gcggtccacg atcggcatgc ctgcccacca ggcggcgtgg
     2221 aagagcgtcg tcgccacctg gcagatgccg ccgcccaccg agggcacggt cgtcacgctg
     2281 ccgttcgtcg tggcgatgcc gtagcccagc ttgtagccgt tcttcagatc gaccgccccg
     2341 atcgtgcccg tgaaggagaa cgtgccgccc ggcggcacca gcgcgccgtt cacgcgctcg
     2401 acggccagct ccacgttgta ggcccggttc gcgatcgagc cggcatacga ggtctgaccc
     2461 gagccgagct tgtccgagat cacgatgctg ctggccatcg ccgccgtgta cttcggctcg
     2521 actggcttgg tgacgatcgc cgcgctggta ctccctttca ggagcgcatc acgcacggcc
     2581 tcggaggact tctgcaggtc cagcgtcacg cccgcctgct cgggagcgac caccttgacc
     2641 gcgccgtcga ggtagcggaa ctgcgcgtcc ttggccggct tttcgatctt gtccttgatg
     2701 ccgtccagcg cctgggtcag cgcggctggg tcgagggaca tagtcagctt ctggctgtcg
     2761 ccgggatgcg cctggaagct gaccgactga ccaatcacct cggccggcag cggcaggctg
     2821 cccccatccc aggtgacggt gaagggtttc gctaccagtt gctgcgcttg gtcggccgcc
     2881 gcgcgggcgt cggcgtcggt gatcgccggt gcgtgagcct gcgtcaccgc gtcgacggcg
     2941 tgctgcccct gcccgagcgc cttgaggatg gcgtctgccg tggccgccgg gtcgaacgcc
     3001 tgtgcgtcct gtcctgacac gaccgcgaac gccttgccat cccacttcac accggcatct
     3061 actccggtgc gcttgattgc gccgtcgagc tggccgaggt aggtagccag ctcctggcgc
     3121 gagagtgtag gcgccgcctg cccggtatcc gcgcgcgcca acatggtgcg cagggtggcg
     3181 ggcgcgatct cccaacgatc gccgccgaag cgaatggaca ctggctgact gatgagcgcg
     3241 ctggcctgcg ccagccccgg ttggaggtcc ttcgcctcca cctgctgcgg caggcccacg
     3301 gtctggatcg cgaccggctc cgaagagagc gcggccaccg agcgctcgat cgcgccgtag
     3361 gtcgcggcgg tgttgatgcc gactccggtc gagcctggat cgatcgtgag cttgccgccg
     3421 gtgtcgaaga catacgtcgc atccaccggc gggcgcgcca cttccggcgc cagcgtccgc
     3481 agggcgtcaa gcgacgcctg ctggtcgacc gagaagacca ccggcacgcg gctcccgcca
     3541 agccaggccg acaggaggtc cgccgagtcg gaccagagat tgccgtcgtg acccagcgcg
     3601 aacgcctggg ccgcggtctg ttcggcatcg tagcgcacgt tcaactggg
//
LOCUS       DASUBI010000800         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_920085, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000800 DASUBI010000000
VERSION     DASUBI010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920085"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFU72_15690"
     CDS             complement(<1..479)
                     /locus_tag="VFU72_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424984.1"
                     /translation="MSRLSALLMLVLAVAALAGCQGPQAPEAGTPGATPARATAAPSP
                     MSTPASIAPATLAPTPSPTPAPTPTPSPTPAATPSATPAPSPTATPSAGQTQPVPIVM
                     YHYVRPDPGPSDPAGQDLSVTPEDFAAEMDYLAAQHYTTMTLGELEAVRERHASLPA"
     gene            complement(544..1776)
                     /locus_tag="VFU72_15695"
     CDS             complement(544..1776)
                     /locus_tag="VFU72_15695"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5424985.1"
                     /translation="MQNRDFRKLWAGQTVSIFGSLITRTALPFAAILTLNASPIELGL
                     LSAADVAPGILAGLVAGAWIDRLRRRPLMIAADLGRAVLLASIPLAYLLGVLHIAQLY
                     VVALLAGTLTTVFEVAYLSYLPSIVGRSQLLEGNSRLAATASVAETASFGLGGWLVQL
                     FSAPLAIVIDAVSFLWSAAALALIRAPEPPPARVDERQGMRREIGEGLRLVLHDRPLR
                     VLAGSTALMHLAFGIGSALYMLYVTRDLGFKPGVLGMIFAVGGVTSLGAAVLTPRLTG
                     RLGETRALAAGLALSALGTVLVILTPGATILGIAFLVGQQLIGDAGGTLFLVNNQSLV
                     QSAAPDRLLGRVNATVRFGTVAAMLIGSLLGGVLGQFAGVRAAYVLSPLATLSAAVWL
                     ARLPATLHARPQPLTVRE"
     gene            complement(1793..>2095)
                     /locus_tag="VFU72_15700"
     CDS             complement(1793..>2095)
                     /locus_tag="VFU72_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424986.1"
                     /translation="RDVFASAHRGLDLPPRSIHELRHSRLAPLGGQPLHYVSRRQGHA
                     SINLTADLYGHMLPDTERDDAETFAAIMATAAQARQSARDQMGTKHAPNGAKTRTK"
BASE COUNT          322 a          717 c          789 g          267 t
ORIGIN      
        1 ttggccggca gcgaggcgtg ccgctcgcgc accgcttcca actcgccgag ggtcatggtg
       61 gtgtagtgct gcgcggccag gtagtccatc tcggcggcga agtcctccgg cgtcaccgag
      121 agatcctggc cggccgggtc actggggccc ggatcggggc gcacgtagtg gtacatcacg
      181 atcgggaccg gctgcgtctg cccggccgac ggcgtggcgg tgggcgaggg tgccggggtg
      241 gcgctcggtg tcgctgccgg agttgggctt ggcgtcggtg tgggagccgg ggtgggcgac
      301 ggggtgggcg ccagtgtcgc cggggcgatt gatgccgggg tcgacatggg cgagggcgcc
      361 gccgtcgcgc gggccggcgt cgctcccggc gtgccggcct cgggcgcctg cggcccctgg
      421 cagccggcga gcgccgccac ggccaggacc agcatcagca gcgccgacaa acggctcacg
      481 gcaacgctcc ctaacgctcg aattcgtccc ctcggggtta acgttcactc agtccggacg
      541 gcactactcg cgcacggtca gcggctgggg gcgggcgtgc agcgtggccg gcagccgcgc
      601 cagccagacg gccgcggaca gcgtcgccag cggcgagagc acgtaggcgg cacgcacgcc
      661 ggcgaactgc cccagcaccc cgcccagcag cgagccaatc agcatcgcgg ccaccgtgcc
      721 aaagcggacg gtggcgttga ctcggccgag cagccggtcc ggcgcggcgc tctgcaccag
      781 gctctggttg ttgaccagga agagcgtgcc gccggcatcg ccgatcaact gctggccgac
      841 caggaaggcg atgcccagga tggtcgcgcc cggggtcagg atcaccagca cggtcccgag
      901 cgccgaaagc gccagcccgg cagccagcgc gcgcgtctcg ccgagccgac cggtcaggcg
      961 cggcgtcagc acggcagcgc ccagcgaggt gacgccaccg acggcgaaga tcatgccgag
     1021 cacgcccggc ttgaagccga ggtcgcgagt gacgtagagc atgtagagcg cgctgccgat
     1081 cccgaacgcc agatgcatca gcgcggtcga gccggccagc acccgcagcg gccggtcgtg
     1141 cagcaccagc cgcagccctt cgccaatctc gcgccgcatc ccctggcgct cgtcgacgcg
     1201 cgcgggcggc ggctccggcg cgcggatcag cgccagtgcg gccgccgacc agaggaagct
     1261 gacggcgtcg atcacgatcg ccagcggcgc gctgaagagc tgcaccagcc agccgcccag
     1321 gccgaacgag gcggtctccg ccaccgaggc ggtcgccgcc agccggctgt tcccctccag
     1381 cagttggctc cgcccgacga tcgaggggag gtaggagagg taggcgacct cgaagacggt
     1441 cgtcagcgtg ccggccagca gcgcgacgac gtagagctgc gcgatgtgca gcacgccgag
     1501 caggtaggcc agcggaatcg aggccagcag cacggcgcgc cccaggtcgg cggcgatcat
     1561 cagggggcgg cggcgcagcc ggtcgatcca ggcgccggcc accaacccgg ccagaatgcc
     1621 cggcgccacg tcggcggcgc tcaagagccc gagctcgatc ggcgaggcgt tcagcgtcaa
     1681 gatggcggcg aagggcagcg ccgtgcgcgt gatcagcgag ccgaagatcg agacggtctg
     1741 ccccgcccag agcttgcgga agtcgcggtt ctgcaagacg ttcggagaag ccctattttg
     1801 tgcgggtttt tgccccgttg ggagcatgct tggtccccat ttggtcccgc gccgactgtc
     1861 tcgcctgcgc tgcggtcgcc atgatggccg cgaaggtctc ggcatcgtcc cgttccgtgt
     1921 ccggcagcat gtggccgtag aggtccgcag tgaggttgat gctggcgtgt ccctgacgcc
     1981 gggaaacata gtgcagcggc tggcccccaa gaggagcgag gcgtgagtgg cgcaactcgt
     2041 ggatcgagcg gggcggcagg tcgagcccgc gatgcgcact ggcgaagaca tccct
//
LOCUS       DASUBI010000801         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_347699, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000801 DASUBI010000000
VERSION     DASUBI010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347699"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFU72_15705"
     CDS             <1..337
                     /locus_tag="VFU72_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEU5424987.1"
                     /translation="GLPGNPVSALVGFQIFVRSALRALSRELEPRPRTVAVVLEHDVA
                     SGDRLEYQRGVVWADEAGVLHARNTGSQASARLMSLVGANAFILIAPREAPYRAGERV
                     PALILQPLG"
     gene            complement(352..1713)
                     /locus_tag="VFU72_15710"
     CDS             complement(352..1713)
                     /locus_tag="VFU72_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5424988.1"
                     /translation="MSDTLDRLLRMVDEAQDEVVQLTQDLVRLPTVNFGRPDSGDELM
                     AAELLRDRLTAGGIDAAIYPSAANRGNFVASLGGDSGPSLLFMSHTDVVPVEDANQWT
                     HPPFGAEIAEGRIWGRGAADMKSTVAAQAMALLILRRAGVALNGTLRFASCADEEAGG
                     AFGFGWMAQHHPELLRADYAVNEGGGVPVTRAGRVIYPISTGEKGRLEAHYRVTGRGY
                     HASQPWNADNAIYKADEVIQRIRAYQPEVSVKVDLFQHLDTLAGIGEAVTVANLEDIL
                     GRVAEQDRNLASMLRAASRMTLVATMIEGGVKSNSVAENCTVVCDIRSLPWQDKEYVR
                     GQLQGIIEGLDGVSLELIETAVSNSSPYDSPFRDKVERATRDALGGAELAFVPGLTVG
                     FTDSRFVRPLGDVAYGFVPASPNEDPSKSGAHNINESFGIESLMTATRFHVALAYHTL
                     VGE"
     gene            1908..2636
                     /locus_tag="VFU72_15715"
     CDS             1908..2636
                     /locus_tag="VFU72_15715"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5424989.1"
                     /translation="MVVESEHVRDVMLPGIETIGPDEPVRVARRRMESTTDRSLIVAD
                     GARPIGIVAWRAISNQNRVPADAPVSEFMTREFPTITPDMPLSQAQNNIAGVDIDNIP
                     VVNEEGQLVGVVPRPVLAHVEQAAQEAPVEEVEPLGTRPPTEMMGPGQTAVSTPAEAA
                     GPDLRTGMSVVGGNGDKIGTVSELLADTAGRATAIVVEHGLLRKKHKRVPVDNISGVS
                     DGTVTLTIDSRDFGLLPNVEDQAS"
     gene            complement(2861..3574)
                     /locus_tag="VFU72_15720"
     CDS             complement(2861..3574)
                     /locus_tag="VFU72_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5424990.1"
                     /translation="MRVTVRYFAAIREAIGSRGETRELPEDTSAGRLVDLLVADYPAI
                     GPLRRSTALMINHAYAQPDQPLREGDEIALIPPVSGGDGGDSAYRVTDGPIDLAQVEA
                     LVADPAAGAVVSFAGTVRDHARGRHVLRLEYEAYPEAAEKILAQIGQEIAARWEVTRV
                     AIVHRTGTLEVGEVSVAIAVSSAHRAEAFDACRYAIDRIKEIVPIWKKEFYAGGERWI
                     GSEAEYQAAYGTSGRADHA"
     gene            complement(3700..3772)
                     /locus_tag="VFU72_15725"
     tRNA            complement(3700..3772)
                     /locus_tag="VFU72_15725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3737..3739),aa:Arg,seq:tct)
     gene            complement(3914..4264)
                     /locus_tag="VFU72_15730"
     CDS             complement(3914..4264)
                     /locus_tag="VFU72_15730"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEU5424991.1"
                     /translation="MPRDPRTYLFDIRRTCDLLTEFTHGKALTDYNSDALLRSAVERQ
                     FEIIGEALGQLLKENPRLAPRSSHARQIISFRNRLIHGYATIDDAVVWGVLQTNVPIL
                     RREIADLLNADEPV"
     gene            complement(4254..4562)
                     /locus_tag="VFU72_15735"
     CDS             complement(4254..4562)
                     /locus_tag="VFU72_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5424992.1"
                     /translation="MRDIVRERLGELRRLCEKHHARRLELFGSAAHGDVALESNDLDF
                     LVEFEDLSPASHAASYFGLLADLEDLFRLPIDLIEIQAIRNPYFLQEVERTRMLLYAA
                     "
BASE COUNT          765 a         1556 c         1586 g          750 t
ORIGIN      
        1 cggcctgccc ggcaacccgg tctcggcgct ggtcggcttc cagatcttcg tgcgctcggc
       61 gctgcgcgcc ttgagccgtg agctggagcc gcgcccacgc acggtggccg tggtgctgga
      121 gcacgacgtc gcgagcggcg accggctgga gtaccagcgc ggcgtcgtct gggccgacga
      181 ggcgggcgtg ctgcacgcgc gcaacaccgg ctcgcaggcc tcggcgcggc tgatgtcgtt
      241 ggtcggcgcc aatgccttca tcctgatcgc cccgcgcgag gcgccctacc gcgccggtga
      301 gcgcgtcccg gcgctcatcc tgcagccgct cggctaacgc gctgcccggc cctattctcc
      361 gaccagcgtg tggtaggcca gcgcgacgtg gaagcgcgtg gcggtcatca ggctctcgat
      421 gccgaacgac tcattgatgt tgtgcgcccc gctcttgctg gggtcctcgt tggggctggc
      481 cggcacgaag ccgtaggcca cgtcgcccag cgggcgcacg aagcgcgagt cggtgaagcc
      541 gacggtgagg cccggcacga acgccagctc ggccccgccc agcgcgtcgc gcgtggctcg
      601 ttcgactttg tcgcggaatg gcgagtcata gggcgagctg ttggagaccg ccgtctcgat
      661 cagctccagg gacaccccat cgagcccctc gatgatcccc tgcagctgac cgcgcacgta
      721 ctccttgtcc tgccagggca gcgagcggat atcgcagacg acggtgcagt tctcggccac
      781 gctgttcgac ttcacgccgc cctcgatcat cgtcgccacc agcgtcatgc gcgaggcggc
      841 gcgcaacatc gaggccaggt tgcgatcctg ctcggcgacg cgtcccagaa tgtcttccag
      901 gttggcgacc gtcaccgcct cgccaatgcc ggccagcgtg tcgagatgct ggaagaggtc
      961 gaccttgacc gagacctcgg gctggtaggc gcggatacgc tggatcacct cgtcggcctt
     1021 gtagatggcg ttgtcggcat tccagggctg cgaggcgtgg tagccgcgcc cggtgacgcg
     1081 gtagtgcgcc tccagccgcc ccttctcgcc cgtgctgatc gggtagatga cgcgcccagc
     1141 acgggttacc gggacgccgc cgccctcgtt gacggcgtag tcggcgcgca gcagctccgg
     1201 gtgatgctgg gccatccagc cgaagccgaa ggcgccgccg gcctcctcgt cggcgcaact
     1261 ggcgaagcgt agcgtgccgt tgagggcgac gccggcccgg cgcaggatca gcagggccat
     1321 cgcctgcgcg gccaccgtcg acttcatatc ggccgcgccg cgtccccaga tgcggccctc
     1381 ggcgatctcg gcgccgaacg gcggatgggt ccactggttg gcgtcctcga ccggcacgac
     1441 atcggtgtgt gacatgaaga ggagcgacgg accgctgtcg ccgcccagcg aggcgacgaa
     1501 gttgccgcgg ttcgccgccg aggggtagat cgcggcatcg atgccgccgg ccgtcagccg
     1561 gtcgcgcagg agctcggcgg ccatcagttc atcgccgctg tcggggcggc cgaagttgac
     1621 ggttggcagg cgcaccagat cctgggtcaa ctgaacgacc tcgtcctggg cctcgtccac
     1681 catccgcagt agtcggtcga gcgtgtcgct catgcgtgtc tccccctgga ctgacgtgcg
     1741 ccggatcaac ggggcggatc gtactcagct tccccgccgc tggcaagagc cgcgccgaga
     1801 caggcaattt ttgcgtggca cactgcttgc ttccgacgca cgcggcacac actccgtgcg
     1861 ccccgatacg agggaagggg cggggagtag gaaaggagcg tctcaccatg gtggttgaga
     1921 gcgaacacgt cagagacgtg atgcttcccg gtatcgagac gatcgggccg gacgagccgg
     1981 tacgggtggc gcgacgacgg atggaatcga caaccgaccg ctcgctgatc gtggccgatg
     2041 gcgcccgtcc gatcggcatc gtagcctggc gcgccatctc caaccagaat cgcgtgccgg
     2101 ccgacgcgcc ggtcagcgag ttcatgacgc gcgagttccc gacgattacg ccggacatgc
     2161 cgctctcgca ggcgcagaac aatatcgcgg gggtcgacat cgacaacatt ccggtggtga
     2221 acgaggaggg tcaactggtc ggcgtggtgc cgcgcccggt gctggcgcat gtcgagcagg
     2281 cggcgcaaga ggcgccggtc gaggaagtcg agccgctggg aaccaggccg ccaaccgaga
     2341 tgatggggcc ggggcagacg gcggtctcga cgcctgccga agcggccggg cccgacctgc
     2401 gaaccgggat gagcgtggtg ggcggcaacg gagacaagat cggcacggtc agcgagttgc
     2461 tggctgatac cgcgggacgc gctaccgcca tcgtggtgga gcacgggctg ctgcgcaaga
     2521 agcacaagcg ggtgccggtc gataacatct ccggggtgag cgacggaacg gtgaccctga
     2581 cgatcgacag ccgcgatttt ggtctcctgc cgaacgtgga agaccaggca agctagcgcg
     2641 gagcgcgcga caactggcga cttcgttaat cctgaaccgt catctcggtc atccccagcc
     2701 gcagcgaggg atctctcctc agcagcaccg cgcagcaagc tcggaactca ggggagattc
     2761 ttcggctgcg gcctgagcat gacagagggg gcgcaaggtc gcgcctcatg gtcgagcgac
     2821 ggcaactgcg gctagtaagg gagatgcttc gggcgtggga ctaagcgtgg tcggcgcgcc
     2881 ccgaggtgcc gtacgcggcc tggtattcgg cctcgctgcc gatccagcgc tcgcccccgg
     2941 cgtagaactc cttcttccag atcggcacga tctccttgat gcggtcgata gcgtagcggc
     3001 aggcgtcgaa ggcctcggcc cggtgcgccg acgagaccgc gatggccacg ctgacctcgc
     3061 ccacttccag cgtgccggta cggtggacga tcgccacgcg cgtcacctcc cagcgcgcgg
     3121 cgatctcctg gccgatctgg gccaggatct tctccgccgc ttcggggtag gcctcgtact
     3181 ccagccgcag cacgtgacgg ccgcgcgcgt ggtcgcgcac cgtgccggcg aagctgacca
     3241 ccgcgccggc ggcgggatca gccaccaggg cctcgacctg cgcaagatcg atcggtccgt
     3301 cggtgacccg atacgcgctg tcgccgccgt ctccgcccga gacgggcggg atcagtgcga
     3361 tctcgtcacc ttcacgcaac ggctgatcgg gctgggcgta cgcatggttg atcatcagcg
     3421 cggtgctgcg gcgcagggga ccgatcgccg ggtagtcggc caccagcaag tcgacgagcc
     3481 gccccgccga ggtgtcctcc ggcagctcgc gcgtctcgcc ccgcgacccg atcgcctccc
     3541 ggatcgccgc gaaatagcgc accgtcaccc gcattcgccc gcctcctcgc tgaccacgcc
     3601 caacgcccgg tcctggacga agtgtaccgt gcccgagtga gccggcaccc accggtcatg
     3661 ccgcgcggcg agcgggcgaa tcgttcatca acacggaggt gcccccggca ggactcgaac
     3721 ctgcgccatg cggcttagaa ggccgccgtt ctgtccactg aactacgggg gcgcgccggg
     3781 tgagcctatg caacagccat ggccaagtac cggtcaaaca gctccatttt agcgtgtacc
     3841 cgccgtcgca gtcgggcgca ttcccccctg cgctgccgaa tcagggtgtt catgccacac
     3901 gacaccagtg ctcctacacc ggttcgtccg cgttgagaag gtcggcgatc tcccgtcgca
     3961 ggatgggcac attcgtttgg agcacacccc agacgaccgc gtcgtcgatc gtcgcgtagc
     4021 cgtggatcaa acggttgcgg aaggagatga tctgccgcgc atggctgctc cgcggtgcca
     4081 gtctcggatt ctcctttagc aattggccga gcgcctcgcc gatgatctcg aattgccgct
     4141 cgaccgccga ccggagcaaa gcgtcggagt tgtagtcggt gagcgccttg ccgtgcgtga
     4201 actcggtgag aagatcacat gtgcgccgaa tatcgaacag gtaggtgcgg gggtcacgcg
     4261 gcatacaaga gcattcgcgt gcgctcaact tcctggagaa agtaaggatt gcgaatggct
     4321 tggatctcga tgagatcgat cggcaatctg aagaggtctt cgaggtctgc gagaagccca
     4381 aaatagctgg cggcgtgaga ggccggtgaa aggtcttcga actcgaccag gaagtcgagg
     4441 tcgttcgatt ccagtgcaac gtcgccatgc gccgctgagc cgaaaagctc caagcgccgg
     4501 gcatgatgct tctcgcagag ccgccggagc tctccgagcc gctctcgcac gatgtcccgc
     4561 atggcacttg ctccacccct ggcgaggcgc ctgcgcgtgc aatcagcgac tctagccagt
     4621 ctctgcgcgt tccgcgccta cgaatgacgc ttagagg
//
LOCUS       DASUBI010000802         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_803616, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000802 DASUBI010000000
VERSION     DASUBI010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803616"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFU72_15740"
     CDS             <1..1152
                     /locus_tag="VFU72_15740"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5424993.1"
                     /translation="ALFPARARARGPEAEARVAGAVQKKAQEVARYVLPLATPAHFYH
                     TINGLTLLRYYVLANQLDVPGEVRYLVNRMVQEVLALDPDFLGAPGYPLDLSILAAED
                     TLEYEALADLRANLDQTGDEPFFTEFDAALGGYDSRLVGYDPNAEHVLADAVRTVLGL
                     PANELSDAAAIARVLDPAQNSYLGHALFLAMNSKLMQTMNHVPFTFQKRISGAEEAQN
                     QRHRGTLSSSPILSAHLRREPDVIVPAELARVPAALDEYHATIQALWDAKNRLLDAGV
                     PAEQVLYLLPNSHHVRFYESGTLLTYFWKWVKRLCYNAQREIFETARQETEQVTAVLP
                     LIGNYVNRPPCVLRAAADQKPRCPEGERYCGVPVWRDYDPATLAERRVL"
     gene            complement(1159..1920)
                     /locus_tag="VFU72_15745"
     CDS             complement(1159..1920)
                     /locus_tag="VFU72_15745"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5424994.1"
                     /translation="MRLQGKVALITGGTSGIGRAMVELFAREGALVTFSGRDREAGRA
                     LGAAVAAAGGEARFVPLDVRRLDELHRVIAETAERHGRLDVQVNNAGAVLAKSVLETS
                     EDEFDLVFATNVKPVFFGTKWAAEVMRRQGGGSIVNTASTSGLRGQRERAAYCGSKGA
                     VIQITRAAALDLASERVRVNCVSPGAIDTPLLRGARFPERPDQDELVAQVGRALPLGR
                     IGSPRDIAAAALYLASDESAWVTGTNIVVDGGATA"
     gene            2041..2232
                     /locus_tag="VFU72_15750"
     CDS             2041..2232
                     /locus_tag="VFU72_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5424995.1"
                     /translation="MTTRVGKRRIDGRTFWYVAWESANGRSTLYWYVDTRDEANQVRH
                     RLRDWDGRDEATTLAVAAD"
     gene            complement(2355..>3889)
                     /locus_tag="VFU72_15755"
     CDS             complement(2355..>3889)
                     /locus_tag="VFU72_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5424996.1"
                     /translation="PAKHKGGQVVYGSSADAQTANPVLSHDTASGAIINLMFQGVVQT
                     SPEDGSPAPSLAKSWDISQDGITYTFTLNDGIKWHDGQPFSADDVKFTYDLFLNPEAN
                     SPRTSTLAERIAKVETNDPAHVTVTLKTPNSSFLVSNMAYGVVPQHILKDVQPKDIAQ
                     HDFSTGKKGVTIGTGPFMFEDWVKDDHITLVKNPSYWQGEPNLEKWIYKVVPNTTVSV
                     QQLKTGELDYASIEPSDIDDMKKQDNVTVHTYDTYSATFWCFQLDPSKTTVFQDKEVR
                     QALLYALDRKAMVEAIDFGYSTVAIGTIPTISWAYNPDGIKLHYPYDMDKANQLLDQA
                     GWAKGSDGIREKNGRKLGFKILSNSGSQPREQYTSVFQQQWKKLGVDATPELIEWNSF
                     LDRITKQKNFDLFFEGFSWGVDPDQTAWFATSSQKGGLNRGNYSNPQVDKLLQDGLAT
                     TDQAKRKDIYTQLENIVMDDLPYVVTDFPKAAYGVNKRMHNYFPNAINSTFNAHQWWV
                     DS"
BASE COUNT          591 a         1337 c         1282 g          679 t
ORIGIN      
        1 gccctcttcc cggcccgggc gcgcgcgcgc ggtcccgagg ccgaggcgcg ggtagccggc
       61 gcggtgcaaa agaaggcgca ggaggtggcg cgctacgtgc tgccgctggc cacgccggcc
      121 cacttctacc acaccatcaa cgggctgacg ctgctgcgct actacgtgct cgccaatcag
      181 ctcgacgtgc ccggtgaggt gcgctacctg gtcaatcgta tggtgcagga ggtgctggcg
      241 ctcgaccccg acttcctcgg tgcccccggc tacccgctcg acctcagcat cctggccgcc
      301 gaggacacgc tggagtacga ggcgctggcc gatctgcgcg ccaacctcga ccagaccggc
      361 gacgagccgt tcttcaccga gttcgacgcc gcgctcggcg gctacgactc gcggctggtc
      421 ggctacgacc ccaacgccga gcatgtcctg gccgacgcgg tgcgcaccgt gctcggcctg
      481 ccggccaacg agctgtccga cgccgccgcc atcgcgcgcg tgctcgaccc ggcccagaac
      541 tcctacctcg gccacgcgct cttcctggcg atgaactcca agctgatgca gaccatgaac
      601 cacgtcccgt ttaccttcca gaagcgcatc agcggggctg aagaggcgca gaaccagcgc
      661 caccgcggca ccctctcctc cagcccgatc ctctcggccc acctgcgccg cgagcccgac
      721 gtgatcgtgc ccgccgagct ggcgcgcgtg cccgccgcgc tggacgagta ccacgccacc
      781 atccaggcgc tctgggacgc caagaaccgg ctgctcgacg ccggtgtgcc ggctgagcag
      841 gtgctctacc tgctgcccaa cagccaccac gtgcgcttct acgagagcgg cacgctcttg
      901 acctacttct ggaagtgggt caagcgcctc tgctacaacg cccagcgcga gatcttcgag
      961 accgcgcgcc aggagaccga gcaggtcacc gccgtgctgc cgctcatcgg gaattacgtc
     1021 aaccgcccgc cctgcgtcct gcgcgcggcc gccgatcaga agccgcgctg ccccgagggc
     1081 gagcgctact gcggcgtgcc ggtctggcgc gattacgacc ccgccacgct ggccgagcgc
     1141 cgcgtcctct gagcgcgctt acgccgtcgc gccgccgtcg accacgatgt tcgtgccggt
     1201 gacccacgcc gactcgtccg aggccagata gagcgcggcg gcggcgatgt cgcgcggcga
     1261 gccgatgcgg cccagcggca gcgcgcgacc gacctgcgcc accagctcgt cctgatccgg
     1321 ccgctccggg aagcgcgccc cgcgcagcag cggcgtgtcg atcgccccgg gcgagacgca
     1381 gttgacgcgc acgcgctcgc tcgccaggtc gagcgccgcc gccctggtga tctggatgac
     1441 cgcccccttg gagccgcagt aggcggcgcg ctcgcgctgg ccgcgcagcc cgctggtgga
     1501 ggcggtgttg acgatcgagc cgcccccctg ccgccgcatg acctcggccg cccatttggt
     1561 gccgaagaag accggcttga cgttggtcgc gaagaccagg tcgaactcgt cctcgctcgt
     1621 ctccagcacc gacttcgcca gcacggcgcc cgcgttgttg acctgcacgt cgagccgacc
     1681 gtgccgctcg gccgtctcgg cgatgacccg gtgcagttca tcgagccggc gtacgtcgag
     1741 tggaacgaag cgcgcctcgc ccccggcggc ggccaccgcc gcgccgagcg cgcggccggc
     1801 ttcccggtcg cggccgctga aggtcaccag cgcaccctcg cgcgcgaaca gctcgaccat
     1861 cgcccggccg attccgctgg tgccgccggt gatcaacgcc accttgccct gcagccgcat
     1921 cgctcctcct ttggtcctgc ctcacgccgc acgggcgcag tatgccgcaa ccgcgcgacg
     1981 cccagcgcgt tgtaggggca caatgaacgt tcaaaccggt tcgcatgggg gagaagtcgc
     2041 ttgaccacgc gcgtcggcaa gcgccgcatc gacgggcgca ccttctggta tgtcgcctgg
     2101 gaatccgcca atgggcggag cacgctctac tggtacgtcg acaccagaga cgaagcgaat
     2161 caggtgcgcc accgcctgag ggattgggac ggccgcgacg aggctaccac cctggccgtc
     2221 gcggccgact gacgccagca gccgagcgag ggcgggtttg aaacccgccc ctacggatcg
     2281 acgtctccct tctcccgcgc acgggagaag gcctggggag gagggctccc cgcggcagac
     2341 caggcccgaa ctgcctacga atccacccac cattggtgag cgttgaaggt cgagttgatc
     2401 gcgttcggga agtagttgtg catccgcttg ttgaccccgt acgccgcctt ggggaagtcg
     2461 gtcaccacgt aggggagatc atccatcaca atgttctcca gctgggtgta aatgtctttg
     2521 cgcttggcct ggtcggtcgt cgccagccca tcctgcagca gcttgtcaac ctgcgggttg
     2581 ctgtagttgc cgcggttcag cccgcctttc tgcgatgagg tggcgaacca agccgtttga
     2641 tcgggatcga caccccagct gaagccctcg aagaagagat cgaagttctt ctgcttggtg
     2701 atccggtcga ggaacgagtt ccactcgatc agctccggcg tcgcgtccac gcccagcttc
     2761 ttccactgct gctggaagac cgacgtgtac tgctcgcgcg gctggctgcc ggagttgctc
     2821 aggatcttga acccgagctt tctgccgttc ttctcgcgga tgccgtcgga gcctttggcc
     2881 caacccgcct gatcgagcag ctgattcgcc ttgtccatgt cgtaggggta gtgcagcttg
     2941 atgccgtccg ggttgtaggc ccaggagatg gtcgggatgg tgccgatggc gaccgtcgag
     3001 tagccgaagt cgatcgcctc gaccatcgcc ttgcggtcga gggcgtagag cagcgcctga
     3061 cgcacctctt tgtcctggaa gacggtcgtc ttcgacggat cgagctggaa gcaccagaag
     3121 gtggccgagt aggtgtcgta ggtgtggacg gtgacgttgt cctgcttctt catgtcgtcg
     3181 atgtcggagg gttcgatcga ggcgtagtcc agctcgccgg tcttgagctg ctgtaccgag
     3241 acggtggtgt tcggcacgac cttatagatc cacttctcga gattcggctc gccctgccag
     3301 tagctcgggt tcttgaccag ggtgatgtgg tcgtccttga cccagtcctc gaacatgaaa
     3361 ggaccggtgc cgatcgtcac gcccttcttg ccggtcgaga agtcgtgctg cgcgatgtcc
     3421 ttcggctgca cgtccttgag aatgtgctgc ggcacgacgc cgtaggccat gttgctgacc
     3481 aggaacgacg aattgggggt cttgagggtg accgtcacgt gcgctggatc gttggtctcg
     3541 accttggcga tgcgctcagc cagcgtcgag gtgcgcggcg agttggcctc cgggttgagg
     3601 aagagatcgt aggtgaactt gacgtcgtcg gcgctgaacg gctggccgtc gtgccacttg
     3661 atgccgtcgt tgagggtgaa ggtgtaggtt atgccgtcct gcgagatgtc ccacgatttg
     3721 gccaggctcg gcgccggcga gccgtcctcg gggctggtct gcaccacgcc ctggaacatc
     3781 aggttgatga tcgcgccgga ggcggtgtcg tgcgagagga ccgggttggc ggtctgcgcg
     3841 tcggcggagc tgccgtagac cacctggccg cccttgtgct tggccggct
//
LOCUS       DASUBI010000803         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_145968, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000803 DASUBI010000000
VERSION     DASUBI010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145968"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFU72_15760"
     CDS             <1..908
                     /locus_tag="VFU72_15760"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5424997.1"
                     /translation="LRLTFGVNPSPLAGREGQFSTSRQLRERLFRELETNLSLRVKPT
                     EQPDIFSVSGRGELHLSILIETMRREGYEFQVSRPEVITRERDGQTQEPVEHLIIDTT
                     EQYVGTVTELVGARRARMLDMTNDGRGNVRLEFAIPTRGLIGLRNAFLTATKGNGSLG
                     SRLMGSEPWHGPISSARAGALVATESGIALTHGLSNAQERGMTFIEPGTEVYEGMIVG
                     QHPRQGDLPVNVCRAKKLTNMRSSTEDIMVRLTPPISLSLEQALDFLADDELLEVTPK
                     HLRLRKRLLTNDERVKARKRAAATS"
     gene            complement(1103..1411)
                     /locus_tag="VFU72_15765"
     CDS             complement(1103..1411)
                     /locus_tag="VFU72_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5424998.1"
                     /translation="MKTYCVDILASATRVLYVGMTNDLERRVWEHKTGDIPGFTSRYG
                     VTRLVFMEDFTDVREAIAREQQLKNWRREKKVRLIEADNPTWRDLSSGWYQSTGEVGD
                     "
     gene            complement(1637..2770)
                     /locus_tag="VFU72_15770"
     CDS             complement(1637..2770)
                     /locus_tag="VFU72_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5424999.1"
                     /translation="MAAAHEVVVIGGGIVGSIAAYRLARAGARVTLVDRADAGHATAA
                     GAGIISPGTSARSGGPVFALAAASVAFYPELLAQLAADGELATGFETVGGLFIALNDQ
                     EAERLPAVMRQAVERAATTSGIGEVTPVDARQARELFPALAEIAAGFHVAGAARVNGR
                     LIRQALQQAAVKRGTRLLLGDARPLRQAKRVVGVEVDGERIPADVIVLAAGAWTRALG
                     EMLGLSIPVFPQRGQILHLDMPAADTSHWPIVMGFHSHYLLTFPEHRVVAGATRESEA
                     GYDYRMTAGGVREALSEALRVAPGLAPATLAEVRVGFRPASPDQLPILGRAPGLENVF
                     VATGHGANGLQLGPYSGAAVADLVLGAPVAVDLAPFGPERFAG"
     gene            complement(2800..>3387)
                     /locus_tag="VFU72_15775"
     CDS             complement(2800..>3387)
                     /locus_tag="VFU72_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU5425000.1"
                     /translation="AETFTIHWRQKQVEYAFRRGLMQQYADYNVCTREALQFTMQELQ
                     VDLSERGQALLMEAFASLPPYPDAPGGLGALREQGHTLAAFSNGVEESLRALLGRADL
                     LPLLDDVVSVDPLQTYKPAPVVYHTLAARLGKPPEETWVVSGNVWDVIGAKGAGLRAA
                     WVKRRRDAIYDPWGIPPDLVVSDLTELAARLAGAG"
BASE COUNT          515 a         1250 c         1143 g          479 t
ORIGIN      
        1 cgctgcgcct gaccttcggc gtcaacccct cgccgctggc cgggcgcgag ggccagttct
       61 cgacctcgcg ccagttgcgc gagcgcctct tccgcgagct ggagacgaac ctctcgctgc
      121 gggtcaagcc aaccgagcag ccggatatct tctcggtctc cgggcgcggc gagctgcacc
      181 tctcgatcct gatcgagacg atgcgccgcg agggctacga gttccaggtc tcgcgcccgg
      241 aggtgatcac gcgcgagcgc gacgggcaga cgcaggagcc ggtcgagcac ctgatcatcg
      301 acaccaccga gcagtacgtc ggcaccgtga ccgagctggt cggcgcacgg cgcgcgcgca
      361 tgctcgacat gaccaacgac gggcgcggca acgtgcggct ggagttcgcc atcccgacgc
      421 gcgggctgat cggcctgcgc aacgccttcc tgaccgccac caaggggaac ggctcgctgg
      481 gctcgcgcct catgggctcc gagccctggc acggcccgat cagctccgcg cgcgccgggg
      541 cgctggtggc gaccgagagc ggcatcgcgc tgacgcacgg gctctccaac gcccaggagc
      601 gcggcatgac cttcatcgag ccgggcaccg aggtctacga gggcatgatc gtggggcagc
      661 acccgcgcca gggcgatctg ccggtcaacg tctgccgtgc caagaagctg accaacatgc
      721 gctccagcac cgaggacatc atggtgcggc tgacgccgcc gatcagcctc agcctggagc
      781 aggcgctcga cttcctggcc gacgacgaac tgctggaggt cacgcccaag cacctgcgcc
      841 tgcgcaagcg cctgctgacc aacgacgaac gcgtcaaggc gagaaagcgc gccgccgcga
      901 catcgtaggc cacctcggga gcggttgcgg ctgggcagga tggatccctc cactccggtc
      961 gggatgacag ggtaagggat gcggcctatc gggcgaggca tgtcctcgtc cacggcctat
     1021 cctcctcgtc agccacaaca ttaaccctgt catcccgacc ggagtggagg gatccgtcct
     1081 ggaagggcac cgcagcccct cgtcagtcgc ctacctcccc cgtgctctga taccagcctg
     1141 aactgaggtc ccgccacgtc gggttgtcgg cctcgatcag ccgcaccttc ttctcccgcc
     1201 gccagttctt gagttgctgc tcgcgagcga ttgcctcgcg cacgtcagtg aagtcctcca
     1261 taaagacgag gcgagtgacg ccgtagcgac tggtgaagcc ggggatgtct ccggtcttgt
     1321 gttcccagac gcgacgctcc aggtcgttgg tcatgccgac gtagagcacg cgggtggcgc
     1381 tggccaagat gtcgacgcag tacgttttca cgcgcaccct cttctaaatg tccgtagcca
     1441 ccgacaatgg tggtgccggg tgaggacgga tccctccact gcgctcggga tgacagggag
     1501 cggtcggggc ctactgggcg agtccggtca tcgaccaagg ctcatcttcc tcgtcagccg
     1561 gccattagcc ctgtcatcct gagcttgtcg aaggatccgt cctcacccgg cactcaacgt
     1621 cgtcgatggg tggccctcac cccgcgaagc gctccggccc gaacggcgcc aggtcgaccg
     1681 cgaccggcgc acccagcacc agatcggcca cggcggcgcc cgagtagggg ccgagctgca
     1741 ggccgttggc gccgtgcccc gtggcgacga acacgttctc cagccccggc gcccggccaa
     1801 ggatcggcaa ttggtccgga ctggccgggc ggaagccgac gcgcacctcg gccagcgtgg
     1861 ccggcgccag ccccggcgcg acccgcagcg cctcgctcag cgcctcgcgc acgccgcccg
     1921 ccgtcatgcg gtagtcgtag cccgcctccg actcgcgcgt cgccccggcc accacacgat
     1981 gctcggggaa ggtcagcagg taatgcgagt ggaagcccat cacgatcggc cagtggctgg
     2041 tgtcggccgc gggcatgtcc aggtgcagga tctgcccacg ctgggggaag accggaatgg
     2101 ataggccgag catctccccc agcgcccgcg tccaggcgcc cgccgccaga acgatcacgt
     2161 ccgccggtat ccgctccccg tcgacctcga cgccgacgac gcgcttcgcc tggcgcaggg
     2221 gccgcgcgtc gcccagcagc agccgcgtgc cgcgcttgac cgccgcctgc tgcagcgcct
     2281 gacggatcag ccgcccgttg acgcgcgccg cgcccgcgac gtggaagccc gccgcgatct
     2341 cggccagcgc cgggaagagc tcacgcgcct gccgcgcgtc gaccggcgtc acctcgccga
     2401 tgccggatgt cgtggccgcg cgctcgacgg cctggcgcat caccgccggc agccgctcgg
     2461 cctcctggtc attcagcgcg atgaagaggc caccgaccgt ctcgaagccg gttgcgagct
     2521 cgccgtcggc ggcaagctgc gccagcagtt cggggtagaa ggcgaccgag gcggcggcca
     2581 gcgcgaagac cggaccgccc gagcgggcgc tggtgccggg cgagatgatg ccggccccgg
     2641 cggcggtggc gtggcccgcg tcggcccggt cgaccagcgt gacgcgcgcc ccggcgcgtg
     2701 ccagccggta ggccgcaatg gagccgacga tgccgccgcc gatcaccacc acctcgtgcg
     2761 ctgccgccac cgctgcctcc ctcacccttg ccgcgacgct cagccggccc ccgccaggcg
     2821 cgcggccagc tcggtcagat cgctcacgac caggtcgggc gggatgcccc acggatcgta
     2881 gatggcgtcg cggcggcgct tcacccaggc ggcgcgcagc cccgcccctt tggcgccgat
     2941 cacatcccag acgttgccgg agacgaccca ggtctcctcg ggcggcttgc ccagtcgcgc
     3001 ggccagcgtg tgatagacga ccggcgccgg cttgtaggtc tggagcggat cgacgctgac
     3061 cacgtcgtcc agcagcggca gcaggtcggc gcggcccagc agagcgcgca ggctctcctc
     3121 gacgccgttg gagaaggcgg ccagcgtgtg cccctgctcg cgcagcgcac ccagccctcc
     3181 cggcgcgtcg gggtagggcg gtaggctcgc gaacgcctcc atcagcagcg cctgcccccg
     3241 ctccgacagg tcgacctgca gttcttgcat ggtgaactgg agcgcctcgc gcgtgcagac
     3301 gttatagtcg gcgtactgct gcatcagccc gcgccggaag gcgtactcga cctgcttctg
     3361 gcgccagtgg atggtgaagg tctcggc
//
LOCUS       DASUBI010000804         4909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_560174, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000804 DASUBI010000000
VERSION     DASUBI010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560174"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1300
                     /locus_tag="VFU72_15780"
     CDS             173..1300
                     /locus_tag="VFU72_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU5425001.1"
                     /translation="MVARTYHIQVDQSKPLAQEAETGHNRWHEDIAPIFEVEEGDRVI
                     FDTRDAFDGQLNPSSTDADVGALRLGPVHPLTGPVAVKGAKPGDLLEIKLLEIDCDPW
                     DNWGYTAEVPGFGFLRDVFPDPYIVHWHLVGDYAESPQLPGVRIPLAPFPGIAGLAPS
                     TELRLRATEREAECTGRGGVALPPDALDAVPPHGRICEEGLRTVPPREVGGNMDIKQL
                     TPGASLFLPVYTAGGLFSTGDVHFAQGDCEACGTAIEMRSRVHVEFKVHPGEAARRNI
                     RSPQFMRGEYFTSPLMAAPRRFYATTGMSFQPEGANESEDLTLSARNALLSLIDFIGT
                     KGYSPQQAYALCSVAADLRVSQVVDVPNFIVSALLPLDIFV"
     gene            1614..2531
                     /locus_tag="VFU72_15785"
     CDS             1614..2531
                     /locus_tag="VFU72_15785"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5425002.1"
                     /translation="MDERSPAALFINTRSRRGGEEYERAVGLLREAGVPLAACRAVED
                     PAELPELVAEAVASGCRAVLVGGGDGTLSSVIASFVGGSAVLGILPVGTGNLVAKALG
                     IDDLPAAVAAIAGGRVAEIDVCRAGERYFLNSLSLGLAAAFRRDAPRALKQRMGMLAY
                     AIAAGRTFLHAQPFFARLTIDGQGIAAICHDVVATNGRFIGPNLPAGPDAAIDDHELI
                     VFTLGGRGRTRLLIQAVKVWRGRHAGNPRFHYRITSGLTVVADPPQRYLLDGEPGGVT
                     PVTIRLLPHAVKVFAPGVEPASAPRAVTD"
     gene            complement(2528..3289)
                     /gene="cofE"
                     /locus_tag="VFU72_15790"
     CDS             complement(2528..3289)
                     /gene="cofE"
                     /locus_tag="VFU72_15790"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEU5425003.1"
                     /translation="MSAEVRIIGVDGIPEAHPGDDPAALIAGGLQHSGLSLERGDVVV
                     VTQKFVSKAEGRLVNLDEVEPSAFATMWAERFGKDARQVEVVLRESARIVRMDRGLII
                     AETPHGLVCANAGVDASNVAGSAGVSLLPIDSDESARGIRQRLGERFGFEPAVIITDS
                     FGRPWRRGIVNIAIGSAGINPLLDYRGQFDEFGYELHATTMAIIDEIASAAELVMGKL
                     DRRPVALVRGYAYQPGEDGAGAIALVREPESDMFR"
     gene            complement(3336..3734)
                     /locus_tag="VFU72_15795"
     CDS             complement(3336..3734)
                     /locus_tag="VFU72_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5425004.1"
                     /translation="MELSDKVRAFLEAPRFAVLATLNANGTAQQSTMWFELQDDSIMM
                     NTAAGRKKVANMERDPRVSLCIPDGYSYVTLRGRVELNPDREQGQQDIARLAVRYHGP
                     EKAQEFIPDYRKQHRISIRMTVEQVAAHGI"
     gene            3883..4191
                     /locus_tag="VFU72_15800"
     CDS             3883..4191
                     /locus_tag="VFU72_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5425005.1"
                     /translation="MAESMYASVTRLRVKNGEDRAIKQYFTQWEKESDPIPQGVSSAY
                     LIKADDDPETYFMVVTFENQDALQTYEQQRGQQLAKLQPLVTAQPEQHQGEIVSTIEG
                     "
     gene            complement(4343..>4909)
                     /locus_tag="VFU72_15805"
     CDS             complement(4343..>4909)
                     /locus_tag="VFU72_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5425006.1"
                     /translation="DAPSDVADLTGQLAQQLAAIHGIDAARHDLTFLRSAEEAVAHTL
                     RQATARSADPLERRIRDVLAAGMPPSRNWPVLLHGDYWPGNALWRHGRLAAIIDWEDA
                     AAGDPLADLANSRLELLWACGEEAMRLFTLRYLALTSIDATALPAWDLWAALRPLGRF
                     EDWAPDAAARATLRDRHRWFVEQALPRL"
BASE COUNT          736 a         1734 c         1655 g          784 t
ORIGIN      
        1 cttgcccgtt cacccgcgca cgctggtctc accggcggca ttgtagccca catgagccga
       61 aacgccagcc gggcagcccg ctttgccccg gcacttgccc tcttgacggg acggcgcgct
      121 catgcacgat atcgccacgg atcggcacaa cgtagccgga gaggagcgag ccatggtcgc
      181 gcgcacctac catatccagg tcgatcagtc gaagccgctg gcgcaagagg cggagaccgg
      241 gcataaccgt tggcacgaag acatcgcccc gatcttcgaa gtcgaggagg gcgatcgcgt
      301 gatcttcgac acgcgtgacg ccttcgacgg ccagctgaac ccgtcctcta ccgatgccga
      361 cgtcggcgcc ctgcggctcg gcccggtcca tccgctgacc ggccccgtcg cggttaaagg
      421 agccaaaccc ggcgacctgc tggagatcaa gctgctggag atcgactgcg acccctggga
      481 caactggggc tacaccgctg aagtaccggg cttcggtttc ctgcgcgatg tcttccccga
      541 cccctacatc gtccactggc acctagtggg cgactacgcc gagtcgccgc agctccccgg
      601 cgtgcgcatc ccgctggcgc ccttccccgg catcgccggg ctggcgccct cgacggagct
      661 ccggctgcgc gccaccgaac gggaggccga gtgcacgggg cgcggcgggg tggccctgcc
      721 gccggacgcg ctggacgcgg tgccgccgca cgggcgcatc tgcgaggagg ggctgcgcac
      781 cgtgccgccg cgcgaggtcg gcggcaacat ggacatcaag cagctgaccc ccggcgccag
      841 tctcttcctg ccggtctata ccgcgggcgg cctcttctcg accggcgacg tccacttcgc
      901 ccagggcgac tgcgaggcct gcggcacggc catcgagatg cgctcgcgcg tccacgtcga
      961 gttcaaggta catccgggcg aggccgcgcg gcgcaacatc cgctcgccgc agttcatgcg
     1021 cggcgagtac ttcaccagcc cgctgatggc cgcgccgcgc cgcttctacg cgaccaccgg
     1081 catgtcgttc cagccggagg gcgctaacga gtcggaggac ctgacgctct cggcacgcaa
     1141 cgcgctgctc agcctgatcg acttcatcgg cacgaagggc tactcgccgc agcaggcgta
     1201 cgccctctgc agcgtggcgg ctgacctccg cgtgagccag gtggtggacg tgccgaactt
     1261 catcgtcagc gcgctgctgc cgctggatat cttcgtgtag aaggcctctg cccttcggtg
     1321 gcgttgcctg ccgcgtgggg cacggatcct tcgacaagct caggatgaca ggggaaaggg
     1381 gatgacctac cagacgaaat gcatccttgt cgtctgcccc cccaatcaac cctgtcttcc
     1441 cgaccgcagt ggagggatcc gtgtcccgcg cgctacccat cggcaccggc gcgcgccccg
     1501 cttccccccg caccacgctc ggaacgaaac ctgcatagca atgcccgccc gagtctctcg
     1561 tgacccgggt aatcgtcgat gacccgcgac caagcgagac tgcggtaccg cgcatggacg
     1621 agcgttctcc cgcggccctc ttcatcaata ctcgttcccg acgcggcggc gaggagtacg
     1681 aacgcgccgt cggcttgctg cgcgaggcgg gagtgccgct ggccgcctgc cgggcggtcg
     1741 aggatcccgc cgagctgccc gagctggtgg ccgaggcggt cgcgagcggc tgccgtgcgg
     1801 tgctggtcgg cggcggcgac ggcacgctct ccagcgtgat cgcgtcgttc gtcggcggct
     1861 cggcggtgct cggcatcctg cccgtgggaa ccggcaacct ggtcgcaaag gcgctgggga
     1921 tcgacgatct gccggcagcg gtggcggcca tcgccggggg ccgggtggcc gagatcgacg
     1981 tctgccgcgc cggcgagcgc tacttcctca actcgctgag cctcggactg gccgccgcct
     2041 ttcgccgcga tgccccgcgc gcgctcaagc agcggatggg catgctggcc tatgccatcg
     2101 ccgccgggcg cacctttctg cacgctcagc ccttcttcgc gcggctcacg attgatgggc
     2161 agggcattgc cgccatctgc cacgacgtgg tggcgaccaa cggccgtttc atcgggccga
     2221 atctgccggc cggacccgat gcggcgatcg acgaccatga gctgatcgtc ttcaccctgg
     2281 gcggccgcgg ccggacgcgg ctgctgatcc aggcggtcaa ggtgtggcgc gggcgccacg
     2341 ccggcaatcc gcgctttcac taccgcatca ccagcgggct cacggtcgtc gccgacccgc
     2401 cccagcgcta cctgctcgac ggcgagcccg gcggcgtcac tcccgtcact atccgcctgc
     2461 tccctcatgc ggtgaaggtc tttgcgccgg gtgtcgagcc cgcgtcggcg ccccgcgctg
     2521 tgacggacta gcggaacatg tcgctctcgg gctcgcgcac cagagcaatt gcacccgccc
     2581 cgtcctcgcc gggctggtat gcgtagccgc gcaccagcgc caccgggcgg cggtcgagct
     2641 tgcccatcac caattcggcg gcggaggcga tctcgtcgat gatcgccatg gtggtggcgt
     2701 gcagctcgta gccgaactcg tcaaactggc cgcggtagtc gagcagcggg ttgatccctg
     2761 ccgagccgat ggcgatgttg acgatgccgc gccgccaggg acgcccgaac gagtcggtga
     2821 tgatcaccgc cggctcgaag ccgaagcgct cacccagccg ctgccggatg ccgcgcgccg
     2881 actcgtcgga gtcgatcggc aggaggctga cacccgcact gccggccacg ttggaggcgt
     2941 cgacgccggc gttggcgcag accagcccgt gcggcgtctc ggcgatgatc aggccgcgat
     3001 ccatgcgcac gatgcgcgcg ctctcgcgca gcaccacctc cacctgacgc gcgtccttgc
     3061 cgaagcgctc cgcccacatg gtcgcgaagg ctgacggctc gacctcgtcg aggttcacca
     3121 gccgcccctc ggccttggag acgaacttct gggtgacgac gacgacgtcg ccgcgctcca
     3181 ggctcagccc gctgtgctgc agcccgccgg cgatcagcgc ggccgggtca tcgccggggt
     3241 gcgcttccgg aataccgtcg acgccgatga tccgcacttc cgcgctcatg gcgagcgctc
     3301 cttccctcat ctacccctgc ggcggtgcag gcgccttaga tgccgtgcgc cgcgacctgc
     3361 tcgacggtca tccggatcga gatgcggtgc tgtttgcggt agtccggaat gaactcctgt
     3421 gccttctccg ggccatgata gcgcacggcg agacgcgcga tatcctgttg cccttgctcc
     3481 cgatccggat tcagctcgac ccgcccgcgc agcgtgacgt agctgtaccc gtcggggatg
     3541 cagagcgaga cgcgtgggtc gcgttccata ttcgccacct tcttgcgccc ggcggcggtg
     3601 ttcatcatga tgctgtcgtc ctgcagctcg aaccacatgg tcgactgctg cgccgtgccg
     3661 ttcgcgttga gggtcgccag caccgcgaag cgcggcgcct ccagaaaggc tcgcaccttg
     3721 tcgctcagct ccatcaagct ccctttcgct gcgtcgattc cagcggacgc cgtgccgccc
     3781 gcgcgatgct agcaggcttt gggccagcgt ggggagttga cgcgctggct tcggtcgtgc
     3841 tgtatggtgc ggtgtgtgcc gtgggacgat gaaggagtcg taatggccga gagtatgtac
     3901 gcgtccgtga ccagattgcg ggtcaagaac ggcgaggacc gcgccatcaa gcagtatttc
     3961 acccagtggg agaaggagag cgatcccatc ccgcaggggg tcagctcggc ctatctgatc
     4021 aaggccgacg acgacccgga gacctacttc atggtcgtca ccttcgagaa ccaggacgcc
     4081 ctacagacct acgaacagca gcgcggccag cagctcgcca agctgcaacc gctggtgacc
     4141 gcccagccgg agcagcacca gggtgagatc gtctcaacca tcgaggggtg aacgggcccc
     4201 cgcgtattca acccgcttgt aggggcagac ctccgtgtct gccctcgttg ccctttcacc
     4261 tgtcctcact gaagccgccg acatttcccg tatccccgtt gccctgccgt agggggagaa
     4321 gggcagacac ggaggtctgc ccctacaagc ggggtagcgc ctgctcgacg aaccagcggt
     4381 gccggtcgcg cagggtggcg cgggcggcgg cgtcgggcgc ccagtcctcg aagcggccca
     4441 gcgggcgcag cgcggcccag agatcccagg cggggagcgc ggtggcgtcg atcgaggtca
     4501 gggccaggta gcgtagcgtg aagaggcgca tcgcctcctc gccgcaggcc cagaggagct
     4561 ccagccggga attggcgagg tcggccagcg gatcgccggc ggcggcgtcc tcccagtcga
     4621 tgatcgcggc cagcctgccg tggcgccaga gcgcgttgcc cggccagtag tcgccgtgga
     4681 gcagcacggg ccaattgcgc gacggcggca tccccgccgc caagacgtct cggatgcgcc
     4741 gctcaagtgg gtctgctgaa cgcgcggtgg cttgacggag cgtgtgcgcc actgcttctt
     4801 cggccgagcg taggaaggtc aggtcgtgcc gggcggcgtc gatgccgtgg atggcggcga
     4861 gctgctgggc gagctggccg gtgagatccg cgacgtcgct cggcgcgtc
//
LOCUS       DASUBI010000805         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1028778, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000805 DASUBI010000000
VERSION     DASUBI010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028778"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..423)
                     /locus_tag="VFU72_15810"
     CDS             complement(79..423)
                     /locus_tag="VFU72_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425007.1"
                     /translation="MQPPRSRRRRERPSDAQILIAVILEGGAAILTALLLGIALRLSG
                     TEPHGWLARLAQATDLLTRPFENLPWLAGKPALILITLLVTLAWLALLGIVRGWEAES
                     RDHPRFSHRFRP"
     gene            complement(392..1621)
                     /gene="coaBC"
                     /locus_tag="VFU72_15815"
     CDS             complement(392..1621)
                     /gene="coaBC"
                     /locus_tag="VFU72_15815"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5425008.1"
                     /translation="MSGILRGKRIILGVSGGIAAYKAIELARDLTLAGALVDVMLTHA
                     ALEFVRPLPFETLTRRPVYTDVFIPWTEEQHGHITMADEADLMLVAPATANTIARLAA
                     GLADDMLTVSALATTAPLVIAPAMDHKMYLHPATQANLETLQRRGARIIGPDRGPLAS
                     GAVGFGRLVPVERMLDALRATLARNGPLHGRAAVVSAGPTREPIDPIRYLTNRSSGKM
                     GYAIAQALLDAGAAVTLVSGPTALRPPSEATFVPVESAGQMADVVMAAVAGADVLVMA
                     AAVADYTPAQVADQKIKKGESELTLRLGRTLDILATVEQPGLIKVGFAAETTALLDSA
                     QEKLRRKHLDLIVANDAVGTMGSDESRAFFLMPGSEPLELPPLGKDELAERLVEQITD
                     LLESKADHAASSIAPTA"
     gene            complement(1637..>2072)
                     /locus_tag="VFU72_15820"
     CDS             complement(1637..>2072)
                     /locus_tag="VFU72_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5425009.1"
                     /translation="SKGLAAPVGSMLTGPADFIERAREIRRMVGGAMRQSGVIAAAGV
                     VALTEMVARLPEDHARARRLAQGLDELPGMRVDLDAVQTNIIFFKTEPLVPHPEFIEQ
                     LKREGVLVSNYGDRGLRMLTHYMIDDAAVEQAIQAAARALRG"
BASE COUNT          309 a          685 c          765 g          313 t
ORIGIN      
        1 cgcgcgtcgc gccggccatg accggggacg cgcacgaagc ttgccgctag ctggccagtc
       61 tagcagacga ccggcggctc agggacggaa gcgatgcgag aagcgagggt ggtcgcggct
      121 ctcggcctcc cagccgcgca cgatgccgag gagcgccagc caggcgagcg tgaccagaag
      181 ggtgatgagg atcagtgcgg gcttcccggc caaccagggg aggttttcga aggggcgggt
      241 gagcaggtcg gtggcctgtg ccagccgggc gagccagccg tggggttcgg ttccgctcag
      301 gcgcaaggcg atgccgagca gcagcgcggt caggatggcc gcgccgcctt cgaggatgac
      361 ggcgatcaga atctgcgcgt cggaggggcg ctcacgccgt cggcgcgatc gaggaggctg
      421 catgatcggc cttgctctcc aataggtcgg taatctgctc gaccagccgc tcggccaatt
      481 cgtccttacc gagagggggc agctccagcg gctcactacc cggcatcagg aagaaggcac
      541 ggctctcgtc gctgcccatc gttccgacgg cgtcgttggc cacgatcagg tcgaggtgtt
      601 tgcgccgcag cttctcctgg gccgaatcca gcagggcggt ggtctcggcg gcgaagccga
      661 ccttgatcag gcccggttgc tcgacggtcg ccaggatatc cagcgtgcgg ccgagtcgga
      721 gcgtcagctc gctctcgccc ttcttgatct tctgatcggc tacctgggcg ggagtgtagt
      781 cggccacagc cgcggccatc actaggacgt cggcgcccgc cacggccgcc atgaccacgt
      841 cggccatctg tccggcgctc tcgaccggga cgaacgtcgc ctcgctcggt gggcgcaggg
      901 cggtcggacc ggagaccagc gtgaccgcgg cgccggcgtc gagcagcgcc tgcgcgatgg
      961 cgtagcccat cttgccggag gaccggttcg tcaggtagcg gatggggtcg atgggctcgc
     1021 gagtcggccc agcgctgacg accgcggcgc ggccgtgcag cggaccgttg cgcgccagcg
     1081 tagcgcgcag cgcgtccagc atgcgctcca ccgggaccag gcggccgaag ccgaccgcgc
     1141 cggaggccag cgggccgcga tctggcccga tgatgcgcgc gccgcgccgc tgcaaggtct
     1201 cgaggttggc ctgggtggcc gggtgcaagt acatcttgtg atccatggcc ggggcgatga
     1261 cgagcggcgc ggtggtagcc agcgcgctga cggtcagcat gtcgtcggcc aggccggcgg
     1321 cgagcctggc gatggtgttg gcagtggccg gcgcgaccag catcaaatcg gcttcgtcgg
     1381 ccatggtgat gtggccgtgc tgctcctcgg tccaggggat gaagacgtcg gtgtagaccg
     1441 ggcggcgagt cagcgtctcg aaggggagtg ggcgcacgaa ctccagcgcg gcatgagtca
     1501 gcatcacgtc gaccagcgcg ccggccagcg tcaggtcgcg ggcaagctcg atcgccttgt
     1561 aggcggcgat gccgccgctg acccccagga tgatgcgctt gccgcgaaga atgccggaca
     1621 tcgctcccgc ctggtgctag ccgcgcagcg cgcgggccgc ggcctggatt gcctgctcga
     1681 ccgccgcgtc gtcgatcatg tagtgggtca gcatgcgcag cccacggtcg ccgtagtttg
     1741 agaccagcac gccctcgcgc ttgagctgct caatgaactc cgggtgcggc accagcggct
     1801 cggtcttgaa gaagatgatg ttggtctgta ccgcgtcgag gtcgaccctc atgccgggca
     1861 gctcgtcgag cccctgggcc agccgccgcg cccgcgcgtg gtcctcgggc agccgagcga
     1921 ccatctcggt cagcgcgacg acaccggcgg cggcgatcac ccccgactgg cgcatggcac
     1981 cgccgaccat gcggcggatc tcccgcgctc gctcaatgaa atcggccggc ccggtcagca
     2041 tcgagccgac cggtgctgcc aggcctttgg ag
//
LOCUS       DASUBI010000806         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1179649, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000806 DASUBI010000000
VERSION     DASUBI010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179649"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFU72_15825"
     CDS             <1..258
                     /locus_tag="VFU72_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425010.1"
                     /translation="VGGHELRSGRRQTVLALAMLALFLLTLIWSPLTDFFALTPLPFT
                     AHAMLVLVVIGWAASLRFLWRFDLRALASSLRRWPARTRER"
     gene            351..1385
                     /gene="pdxA"
                     /locus_tag="VFU72_15830"
     CDS             351..1385
                     /gene="pdxA"
                     /locus_tag="VFU72_15830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU5425011.1"
                     /translation="MRHASDSRPLLAITMGDPAGIGPEIVLKALRHPEVFTSARPLVL
                     GDRRILRRAAEWVGPPTPEIEVVRTPAEAHFGPGTLTLLDLENASPAACPPGEISAAA
                     GRAAVEYVLTATDLALEGRIDAIVTAPLNKAAMNAAGFHYAGHTELLAERTGTPNVKM
                     LLVGPKLRVIHVSTHVALTEAIRRITAANVLAAIELAQESTQALGIPRPRIAVAGLNP
                     HASEGGLFGDEEARAVVPAIAEARARGYEVSDPQPPDTVFLRAVNGAYDVVVAMYHDQ
                     GHIPMKLLAFDSGVNVSIGLPIIRTSVDHGTAFDIAGTGKAREASLLAAIDIAVKMAE
                     ARRLREGGAR"
     gene            1382..2665
                     /gene="larA"
                     /locus_tag="VFU72_15835"
     CDS             1382..2665
                     /gene="larA"
                     /locus_tag="VFU72_15835"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEU5425012.1"
                     /translation="MSDQTRARVAIPYGDGAIEVDIPRRNLLAVLSPNPVAPASDPRA
                     EIVRALAEPIAATPLREAAKGARQVAILADDLTRQTPVQLIIPALLDELGRAGVRDEQ
                     VTVVIALGTHRPMTEEEIELRFGAGVTGRVRVINNPWWDLEQLADLGVTPNGTPIVVS
                     RLVLEADFVIGLGSIVPHHIPGYSAGAKIVQPGVSGAATTGATHYFSTRTRRSYLGEV
                     ENPVRHEMERIADQVGLSAILNTVLDSAGRVVQTFYGDHRAAFRAGVAVSREVYGIAL
                     ADQTDIVIAGSHPCDIEFWQAHKALYPADIAVREGGTVIVMTPCPEGVSVTHQEMLRF
                     TGLPSRKIETLIERGTIADVVSGGLALAWAKIRERERVSLVSGGISGEEARALGFTLF
                     PSADEALAEALRRHGPDATVTVMTHAPELLPILAR"
     gene            2669..>3603
                     /locus_tag="VFU72_15840"
     CDS             2669..>3603
                     /locus_tag="VFU72_15840"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU5425013.1"
                     /translation="MAQLTIVADDLTGAADSAAPFALAGYATYVALDPSRVPEADVLA
                     LATDSRHLPAERAYDAVKQAVSALPERRWLYKKIDSTLRGQPAAELAALMAAGERTRA
                     LIAPAFPAQGRTTVAGRQLVGGRPLEETPFARQVFSSDLLEHFPTAQLIDLQTVWEGV
                     PAVIARLEEEVNGLWIGDAETDDELRTLARAALDGRVDVVCGSGGLSRALSTLAPFRA
                     GSCPLVAGRSGGPVLVVAGSRHPASTAQVEALGAQGTVVVEPQASLDPAISALRAGRA
                     ALLACRHGERRVDSKGFGRDLAQAVTQIARAVSLGG"
BASE COUNT          547 a         1212 c         1290 g          554 t
ORIGIN      
        1 gtcggtgggc atgagctgcg ctcagggcgc cggcagaccg tactggcact ggcgatgctc
       61 gccctcttcc tgctcacgct catctggtcg ccgctgaccg atttcttcgc gctcacgccg
      121 ctccccttca ccgcccacgc gatgctggtg ctggtggtga tcggttgggc ggcgagcctt
      181 cgctttctct ggcggttcga cctgcgcgcg ctcgcctcct cgctgcgccg ctggcccgcc
      241 cggacgcgcg agcggtgaac ggtagccggt ctcatgtcgc gctcttgacg gagagccagt
      301 aatggcgtga gatggtgacg agcgcggccg tggtaagaaa ggagcggcgc gtgcggcacg
      361 ccagtgacag ccgtccactg ctggccatca ccatgggcga tcccgccggg atcggccccg
      421 agatcgtcct gaaagcgctg cggcaccctg aggtgtttac gtccgctcga ccgttggtgc
      481 tcggcgaccg gcgcattctg cgtcgcgcgg cggaatgggt cgggccgccc acgcccgaga
      541 tcgaggtggt gcgcacgccg gcagaggcgc acttcgggcc cgggacgctc acgctgctcg
      601 acctggagaa cgcctcgccg gcggcgtgcc cgcctggcga gatcagcgcg gcggcgggaa
      661 gggcggcggt cgagtacgtc ctgacggcga ctgatctggc actggaaggc cggatcgatg
      721 cgatcgtgac cgcgccgctc aacaaggccg ccatgaacgc ggcgggcttc cactacgccg
      781 gtcacaccga actgctggcc gagcgaaccg gcacccccaa cgtcaagatg ctcctggtcg
      841 gccccaagct gcgggtcatc cacgtcagca cccacgtcgc gctgaccgag gcgattcggc
      901 gcatcaccgc ggccaacgtg ctggccgcca tcgaattagc gcaggagtcg acgcaggctc
      961 tggggattcc cagacctcga atcgcggtgg ccggcttgaa cccgcacgcc agcgagggcg
     1021 ggctcttcgg cgatgaggag gcgcgggcgg tcgtgccggc gatcgccgag gcgcgggcac
     1081 gcggctacga agtgtcagat ccacagccgc cggacacggt cttcctgcgc gcggttaatg
     1141 gcgcctatga tgtggtggtg gcgatgtacc acgaccaggg gcacatcccg atgaagctgc
     1201 tggccttcga cagcggcgtc aacgtcagca tcggcctgcc gatcatccgc acctcggtcg
     1261 accatggcac cgccttcgac atcgcaggga ccgggaaagc ccgcgaggca agcctgttag
     1321 ccgcgatcga catcgcggtg aagatggccg aggcgcgcag gctacgtgaa ggcggcgcga
     1381 gatgagcgac cagacgcgcg caagagtcgc gatcccgtac ggcgacggcg cgatcgaggt
     1441 cgacattccc cggcgcaacc tgctggcggt gctctcgccg aacccggtcg cgccggcatc
     1501 tgatccccgc gccgagatcg tgcgcgcgct ggccgagccg atcgcggcca cgccgctgcg
     1561 tgaggccgcc aaaggcgcac gtcaggtcgc gatcctggcc gacgatctga cccggcaaac
     1621 accggtccag ctcatcatcc ccgcgttgct cgatgagttg gggcgtgcgg gcgtgcgcga
     1681 cgagcaggtg acggtcgtga tcgcgctggg cacgcaccgg ccgatgaccg aagaagagat
     1741 cgaactccgc tttggcgcgg gcgtcaccgg ccgtgtgcgg gtgatcaaca atccctggtg
     1801 ggacctggag cagctggccg atctaggcgt gacgcccaac ggcacgccga tcgtggtcag
     1861 ccgcctggta ctggaggccg atttcgtcat cggcctcggg agcatcgtgc cgcatcacat
     1921 ccccggctac tcggccggag ccaagattgt gcaacccggc gtctccggcg cggcgacgac
     1981 cggagccacg cattacttca gtacgcgcac gcgccgctcc tacctcggcg aggtcgagaa
     2041 cccggtgcgg cacgagatgg agcgcatcgc cgaccaggtc ggactgagtg cgattctgaa
     2101 taccgtgctc gactccgcgg gcagggtggt gcaaacgttc tacggcgatc accgcgcggc
     2161 atttcgtgcc ggggtggcgg tttcgcgcga ggtgtacggg atcgctctcg cggatcagac
     2221 cgacatcgtc atcgccggct cgcacccctg cgacatcgag ttctggcagg cgcacaaggc
     2281 gctctacccg gccgacatcg cggtgcgtga gggcggcacc gtgatcgtga tgacgccctg
     2341 ccccgagggc gtgtcggtga cacatcagga gatgctgcgc ttcaccgggc tgccgtcccg
     2401 caagatcgag acgctgatcg agcgcggcac catcgcggac gtcgtctcgg gcgggctggc
     2461 gttggcctgg gccaagattc gcgagcggga gcgggtgtcg ctggtcagtg gcggcatctc
     2521 cggcgaggag gcgcgggcgc tcggattcac attgttcccg agcgccgacg aggcgctggc
     2581 ggaggcgcta cgccgtcacg gaccggacgc gaccgtcacc gtcatgacgc acgcgcccga
     2641 gctactgccg attctggcga ggtgagcgat ggcccaactg acgatcgtgg ccgatgatct
     2701 gacgggagcc gcggactccg ccgcgccgtt cgcgctggcg ggctacgcca cgtacgtcgc
     2761 gctcgatccg tcgcgagtgc cggaagcgga cgtgctggcg ctcgcgaccg acagtcgtca
     2821 cctgccggcc gagcgtgcct atgacgcggt taagcaggca gtcagcgcgc tgcccgagcg
     2881 gcgctggctc tacaagaaga tcgactcgac gctgcgcggt cagccggccg cagagttagc
     2941 ggcgttgatg gcggccggag agcgcacacg agccctgatc gccccggcct tcccggcaca
     3001 ggggcgcacg acggtggcag gacgacaact ggttggcggc cgtccgctgg aagagacgcc
     3061 gttcgcgcgc caggtcttct cctcagacct gctggagcat ttcccgaccg ctcagttgat
     3121 cgatctgcag acggtgtggg agggcgtgcc cgcggtgatc gcgcgcctgg aggaggaggt
     3181 aaacggcctt tggatcggcg atgccgagac ggacgacgag ctgcggacgc tggcgcgagc
     3241 ggcgctcgac ggccgggtgg atgtcgtctg cggctccggc ggtctgtcgc gcgcgctgag
     3301 tacgctcgcg ccatttcgcg ccgggagttg cccactggtc gcgggccgca gcggtggtcc
     3361 cgtcctggtc gtcgcgggaa gccgccaccc cgccagcacg gcccaggtcg aggcgctggg
     3421 ggctcagggc acggtcgtgg ttgagcctca agcgtcactt gaccccgcga tctcggcact
     3481 gcgcgcgggg cgcgcggcgc tgctggcctg ccgccacggc gagcggcgag tcgactccaa
     3541 aggcttcgga cgcgatctgg ctcaggcagt cactcagatc gcccgcgccg tatccctcgg
     3601 tgg
//
LOCUS       DASUBI010000807         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1279906, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000807 DASUBI010000000
VERSION     DASUBI010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279906"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFU72_15845"
     CDS             complement(<1..454)
                     /locus_tag="VFU72_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425014.1"
                     /translation="MSGRSASLNSALLPRPASLAGVRQQRLERPVLIPLAIGLAMLAV
                     TGTHVAGAYGLLFLTAALFLLVPISPAWALVPVLAVELTTNNFYITSVGMSERLAMTL
                     IACLVGASLMLRTLRSGDPRLKRVLLPAILLIVIAAPINLMTSGSAYAI"
     gene            complement(469..1635)
                     /locus_tag="VFU72_15850"
     CDS             complement(469..1635)
                     /locus_tag="VFU72_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425015.1"
                     /translation="MLGFVKRWWWLLAIGVIAGVGVALAYRDYGPVKYTTTALVQIPS
                     PNAADPSANARAVRDAAANYAAQAASPRTFALVGQALSGQPGLDTQSLLNMSRNKALI
                     IKQTTGSNFVSISVTDTNPAHAKLIADTFAKVFVDDVNTQSQAQSKGRMQGLQQQIDF
                     ARQQLAAGNLYQSQQDLQGKIRDQRTILLQLQSEYQRDLQSQAIASEAQSKNGTAAAP
                     TADQAALATSLQNAVNQQIKEVLANLDQLNQQIQDVQGQIAKLPSGTDPALVAAYASA
                     YSTELTNLTNQYVGAQVDALTASPTLIQAGQAPQPFMSTHLKKLLTFGVGAGVAVAAA
                     FAFLLDLLLKRRRARRSPVRQQVPVNLEELLKSVDRLGRRSAPTPAVDGQIADR"
     gene            complement(1706..>2149)
                     /locus_tag="VFU72_15855"
     CDS             complement(1706..>2149)
                     /locus_tag="VFU72_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5425016.1"
                     /translation="NSRLDELQALVLRVKLHHLDEWNASRRRHVETYGQLLANSGVTL
                     PVAADYGEPVYHLYVIQTEERERAREQLAAHGIATGIHYPTPIHQQEAARGYGRIAGD
                     LRVTERLAPRVLSLPMYAELQPEQLERIATNLLRATANKEVNVAN"
BASE COUNT          337 a          715 c          720 g          377 t
ORIGIN      
        1 gaatggcata ggcgctcccc gaggtcatca ggttgatcgg cgcggcgatc acgatcagga
       61 ggatagccgg caacagcacg cgtttgagcc gcgggtcgcc cgagcgcagt gtgcgcagca
      121 tgagcgaggc gcccaccagg cacgcgatca atgtcatcgc cagccgctcg ctcatgccga
      181 ccgaggtgat gtagaagttg ttggtcgtca attcgaccgc cagcaccggc accaacgccc
      241 aggcagggct gatcggcacc agcaggaaga gcgcggcggt caggaagagc aacccgtacg
      301 cgccggcgac atgcgtgccg gtcacggcca gcatcgccag cccgatcgcc agcgggatca
      361 gaaccggccg ctccaggcgc tgctgccgca cgcccgccag cgaggcgggg cgcggcagca
      421 gcgcgctatt tagacttgcg cttcgtcccg acactccact catcccaact agcgatcggc
      481 aatctggccg tcaaccgctg gcgtcggcgc actccttcgc ccaagccggt cgaccgactt
      541 cagcagctcc tccaggttca ccgggacctg ctgccgcacc ggggaccggc gcgctcgccg
      601 ccgcttcagc agcagatcga gcaagaaggc gaacgccgcg gccaccgcca cgccggcgcc
      661 cacgccgaac gtgagcagct tcttgagatg cgtcgacatg aacggctgcg gcgcctggcc
      721 cgcctgaatg agcgtcgggc tggcggtgag cgcatcgacc tgcgcaccta cgtactggtt
      781 ggtcaggttg gtcagctcgg tcgaataggc cgaggcgtag gcggccacca gcgccgggtc
      841 agtgcccgac gggagcttgg caatctgccc ctgcacgtct tgtatctgct ggttgagctg
      901 atcgagattc gccagcactt ccttgatctg ctggttgacg gcgttttgga gactcgtcgc
      961 caaagccgcc tggtctgctg tcggagctgc cgccgtcccg ttcttgctct gcgcctcgct
     1021 ggcgattgcc tggctctgca gatcgcgttg gtactccgac tgcaactgca gcagtatcgt
     1081 gcgctggtcg cggatcttgc cctgcagatc ctgctggctc tgatacaggt tcccggcggc
     1141 gagctgttgc cgcgcgaagt cgatctgctg ctgcagccct tgcatccggc ccttcgactg
     1201 ggcctgggat tgcgtgttga catcgtcgac aaagaccttg gcgaaggtgt cggcgatcag
     1261 cttggcgtgc gccggattgg tatcggtgac gctgatcgac acgaaattgg agcccgtcgt
     1321 ctgcttgatg atcagcgcct tgttccgcga catgttgagg agcgactgcg tgtcgaggcc
     1381 gggctggccg gacagggcct gccccaccag cgcgaaggtc cgtggcgagg cggcctgggc
     1441 cgcgtagttc gccgccgcgt cgcgcacagc ccgcgcgttg gcggacggat ctgccgcatt
     1501 cggtgaggga atctgcacca gcgcggtggt ggtgtacttc accggaccat agtcgcgata
     1561 ggcgagcgcc acgcccacgc cggcgatcac gccgatggcc agcagccacc accagcgctt
     1621 gacgaagccc agcatcgggt cgcgcccgga ggactcctcg atctctcccg actctaggcc
     1681 gggaggaatg gcggtgccag gtagctcagt ttgcaacgtt gacctcctta ttggccgtcg
     1741 ctcgcagcag attcgtggcg atgcgctcga gctgctccgg ttggagctcg gcgtacatcg
     1801 gcagcgacag gacacgcggc gccagccgct cggtgacgcg caggtctccg gcgatgcgcc
     1861 cgtacccgcg cgctgcttct tgctgatgga tcggcgtcgg gtagtggatg ccagtggcga
     1921 tgccgtgcgc ggccagctgc tcgcgcgccc gctcgcgctc ctcggtctgg atcacgtaga
     1981 ggtgatagac cggctcgccg tagtcggccg ctaccggcag ggtcacgcca ctattcgcca
     2041 ggagttggcc ataggtctcg acgtgccggc ggcggctcgc gttccactcg tcgagatggt
     2101 ggagcttgac ccgcagcacc agcgcctgca gctcgtccag ccgcgagtt
//
LOCUS       DASUBI010000808         6648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1151705, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000808 DASUBI010000000
VERSION     DASUBI010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151705"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFU72_15860"
     CDS             <1..1479
                     /locus_tag="VFU72_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425017.1"
                     /translation="WGDGSISAGTIQGDGGSFVVLGTHTYAEEHDGYNTKVSITDTGG
                     QQATVIGTANVADAPLSATPVAVSATEGASFSGSAATFTDADPNGTLTDYSATIDWGD
                     QTSTTAGDITKNADGSYTVSGGHTYVEEGSYSVTVTINDKGGSTTTAKSTATVADAPL
                     TATGGFTITATEGQASASQTVATFTDADPNGTLSDYSASIDWGDGTTATAGTVSENAD
                     GSFAVNGDHTYGEEGSYTVTVTISDEGGSQTVANDTASVADAGLTATSQNILSGSGTA
                     YNGAVATFKDANPTAPLSDFSATIDWGDSTAAAAGTISQNADGSFTVSGTHTFAALGP
                     HTVTVHIVDEGGSTADATTQILSYAYLGQGSFVLGDQTVANAAPGTTVTWWGAQWDAQ
                     WAGNDTLSGGPAPTDFKGFAQSLSGTPPVAGGSWTTLPGTSPMPPDSVPTYMAVIVTS
                     HVTESGSTITGNIVHLVIVKTDPGYGPNAGHAGTGTIVATVF"
     gene            1561..1785
                     /locus_tag="VFU72_15865"
     CDS             1561..1785
                     /locus_tag="VFU72_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425018.1"
                     /translation="MCPPEHSYRRDHRVYLLSLWREGEAGSWRAALRPAGGGPRRGFA
                     DLEQFAAYVLRLAEDDEWERGPPERDDPPG"
     gene            complement(1875..2333)
                     /locus_tag="VFU72_15870"
     CDS             complement(1875..2333)
                     /locus_tag="VFU72_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEU5425019.1"
                     /translation="MRPFAFSVIRAILDANVLVSGMLGLRRTTSTPGTILYLWFKRSF
                     ELITSDPLITEVERSLSKPFFVGRIDPSDLTLALAALREDAIRTDITTSVSSIATHPE
                     DDLVLAAAISAQAHFLVTGDSMLQRLEHYQSVQIISPRDFLTLLEQTHRT"
     gene            complement(2309..2644)
                     /locus_tag="VFU72_15875"
     CDS             complement(2309..2644)
                     /locus_tag="VFU72_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU5425020.1"
                     /translation="MAATRNQPTIETMQFTEARQQLSQVVNRVARGERRVLLEKSGIP
                     VAAIVSADDLRILTELEARREARFAAISRISEAFADVPVEDLEREITQALSEARAELR
                     AECDPSPSQ"
     gene            complement(2729..3607)
                     /locus_tag="VFU72_15880"
     CDS             complement(2729..3607)
                     /locus_tag="VFU72_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5425021.1"
                     /translation="MVVVGTSLRSEPAAPGQAGPTFEELGFVEWWNALPPGQHTVAGT
                     VKLRRDLDAPQLGDRRTLWLRLPSSYFDSERAYPVIVMHDGQNLFDEATSFLGEEWRV
                     DETLLALEPELEAIVVGVENGGARRLQEYNPWPADTRAGDYLAFLTDTVLPLVRLACA
                     GRTLDEPTQTLILGSSLGALISLYAYFKRPDVFGLCGAMSPPTWQRRLIPYLAGQRHI
                     GGRIYVDHGRLKPLGPGDNADVGCDGQAVRDALLRAGYRLDHDLFYRHDDGAHDEHAW
                     AARLPEALRLLFPVSE"
     gene            3881..4723
                     /locus_tag="VFU72_15885"
     CDS             3881..4723
                     /locus_tag="VFU72_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5425022.1"
                     /translation="MEELVRRLNGLCDELPFHTGWYLKDLRSGAEADRAGALVGPSAS
                     TRKVAVLMTALKQVHEGKLSLDQPVTIQEKYQHNNSGCFQFLQPGFTMPFRDLLIMMI
                     IVSDNTCTGTIVDMVGLDAINAFSRDAGMVGTTHRQNIPPGGLAWDHPVEATNATTPK
                     DVGLLLDQILQGAGDTQAAARLGSTPELCRYAIEIMTWQQITNRFPMFMPPEAKIAHK
                     TGKGVRNFSDVGIIFRGNEPRYILTAYTDRVPPVLEDGRAGRGAAYLLIAQLCRACWD
                     ALGS"
     gene            5127..5855
                     /locus_tag="VFU72_15890"
     CDS             5127..5855
                     /locus_tag="VFU72_15890"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5425023.1"
                     /translation="MIYGRDFWPLAVGWRLVAAPSWSRELELLDQPGQPPELLRGNLR
                     DLARVNRRLGGVRLTRRAIESLAAGRHDTAAPLSVLDVASGGLDIPRALLTGGASLRL
                     VASDLSAAILALDDGEIPRVGADGRNLPFMDDSFDIAICSLVLHHLAPDGALRMLREM
                     ARVSRLGIVVNDLVRSWAGLLGAFGLGCLTRNPLTRHDAPLSVHRAYTRPQLDVLLRG
                     AGLEPVAAHRFLGYRVALAARSRA"
     gene            5852..>6648
                     /locus_tag="VFU72_15895"
     CDS             5852..>6648
                     /locus_tag="VFU72_15895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5425024.1"
                     /translation="MTCQADVIVVGAGPAGAALTIGLARRGREVLLLDQAAFPRDKAC
                     GEFYSPGVAAALARLGLLDTILAQEHRRHAGMTIVRGEDRFALRYAPPRRGLSIRRRL
                     LDQLLVDEAAASGSVVRQRARVSGVLWEDGRATGVRLRGGEELRASFVAGADGHHSTV
                     GRALGLERPPRWPRRLGLIAHFRLNPGALPAAEMHVAGRFYCGLNPVGEGLVNVGLVG
                     PLGTRRRGETAAQHLERLLDALPTARERLAGAVWDGTVRGIGPLAHHA"
BASE COUNT         1053 a         2282 c         2325 g          988 t
ORIGIN      
        1 tggggcgacg gcagcatctc cgccggcacg atccagggcg atggcggctc cttcgtagtt
       61 ctcggcacgc acacctacgc cgaggagcac gacggctaca acaccaaggt ctcgatcacg
      121 gatacgggcg gacagcaggc caccgtgatc ggcaccgcca acgtggctga cgcgccgctg
      181 agcgcgacgc cggtcgcggt gagcgcgaca gagggcgcgt ccttcagcgg atccgcggcg
      241 accttcaccg acgccgatcc gaacgggacg ctgaccgact acagcgccac catcgactgg
      301 ggcgaccaga ccagcacgac ggctggagac atcacgaaga atgcggacgg cagctacacc
      361 gtcagcggcg gccacaccta cgttgaggag ggcagctatt cggtcacggt cacgatcaac
      421 gacaagggcg gtagcacgac aaccgcgaag agcacggcta ccgtggccga cgctccgctg
      481 acggccacgg gcggcttcac catcacggcg accgaggggc aggcgtcggc cagccagacc
      541 gtggcgacct tcaccgacgc cgatcccaat ggcaccttga gcgactacag cgcgagcatc
      601 gactggggtg atgggaccac ggccacggct ggcaccgtca gcgagaatgc ggacggcagc
      661 ttcgcggtca acggcgatca cacctacggc gaagagggga gctacacggt cacggtcacg
      721 atctcggacg agggcggcag tcaaactgtc gccaacgaca ccgcaagcgt ggctgatgcc
      781 gggctaaccg ccacgagcca gaacatcctc tccggcagcg gcacggccta caacggcgcg
      841 gtcgccacgt tcaaggacgc caatccaacg gcgccgctga gcgactttag cgccaccatc
      901 gactggggcg atagcacggc agccgccgcc ggtaccatca gccagaacgc cgacggcagc
      961 ttcaccgtca gcggcacgca caccttcgcc gcgctcggcc cgcacaccgt cacggtccac
     1021 atcgtggacg agggcggcag cacggccgac gccaccaccc agatcctctc ctatgcctac
     1081 ctgggtcagg ggagcttcgt gctgggcgac cagacggtgg ccaacgccgc gcccggcacg
     1141 accgtgacct ggtggggcgc gcagtgggac gcgcagtggg ccggcaacga taccctgtcg
     1201 ggcgggcccg cgccgaccga cttcaagggg ttcgcccaga gtctgagcgg aacgccgccg
     1261 gtggcgggcg ggagctggac gacgctgccg ggcaccagcc cgatgccgcc ggatagtgtc
     1321 ccgacctaca tggcggtgat cgtcaccagc cacgtcaccg agtccggcag cacgatcacc
     1381 ggcaatatcg tgcatctggt gatcgtcaag accgatcccg gctacggccc caacgcgggc
     1441 cacgccggca ccggcaccat cgtggcgacc gtcttctagc ccgtcgcgac aggagagacg
     1501 cggcgtgggg ccaccgggca cgcccggtgg cccctcctga tagctggtaa ggacctatcc
     1561 atgtgtccgc ctgagcactc ataccgccga gaccatcgcg tctacctcct ctcgctctgg
     1621 cgcgaggggg aggcgggaag ctggcgcgcc gccttgcgcc cggctggcgg cggcccgcgc
     1681 cgtggcttcg ccgacctgga gcaattcgcc gcctacgtcc tgcggctggc cgaggacgac
     1741 gaatgggagc gcggcccgcc cgaacgcgac gatccaccgg gctgagcggc cgccagccta
     1801 tgccaccgac ttgaacagca gccgcagcgg gtcggggagg ctggcggccc aggcgcgctc
     1861 gtcgtgcgcg ccggctatgt ccggtgagtt tgttcgagga gcgtcaggaa gtcgcggggg
     1921 ctgataatct gtaccgattg gtaatgttcc agccgttgaa gcatggagtc cccggtgacg
     1981 agaaagtgag cctgggccga aatagccgcg gcgagtacca ggtcgtcttc cggatgggtc
     2041 gcgatgctgg acaccgacgt cgtgatatcc gttcgtatcg cgtcctcgcg cagcgccgcg
     2101 agcgcgagcg ttagatcact cggatcgatc cggcctacga agaagggctt gctgaggctg
     2161 cgttcgacct cggtgatcaa cggatctgac gtgatcaatt cgaagctgcg cttgaaccag
     2221 agatagagta tcgtgcctgg cgtgctggtg gtacggcgca agccgagcat gccagagacc
     2281 agcacattcg cgtccaggat cgcccgtatc actgagaagg cgaagggtcg cactcggcac
     2341 gcaactctgc ccgcgcctcg ctcaacgcct gcgtgatctc ccgctccaga tcttcgaccg
     2401 gcacatccgc gaaggcttcg ctgatgcggc tgatcgccgc gaagcgtgcc tcgcgccggg
     2461 cctcaagctc ggtcaggatt cgcagatcat cggccgacac gatggctgcc accgggatgc
     2521 cgctcttctc caagagaacg cgacgctctc cacgggccac gcgattgacc acctggctga
     2581 gttgctggcg agcctcggtg aactgcatcg tctcgatcgt cggctgattg cgtgtagctg
     2641 ccactgccgg ctcccctcct gccgctgcaa tggtcattat tgcacaattc tgtgcacaag
     2701 cgcacggcct cgcgtctcaa accatccttc actcggacac cggaaagagc agccgcagcg
     2761 cttcggggag gcgggcggcc caggcgtgct cgtcgtgggc gccgtcgtcg tggcggtaga
     2821 agaggtcgtg gtcgaggcgg tagccggcgc gcagcagcgc atcgcgcacc gcctggccgt
     2881 cgcagccgac atcggcgttg tcgcccggtc ccagcggttt gagccggccg tgatcgacat
     2941 aaatccgacc gccgatgtgt cgctgcccgg ccaggtaggg gatgaggcgt ctctgccagg
     3001 ttggggggct catggcgccg cagaggccga agacatcggg ccgcttgaag taggcgtaga
     3061 gcgagatcag cgcgcccagg ctggagccca ggatcagcgt ctgggtcggc tcgtccagcg
     3121 tgcgcccggc gcaggccagg cgcaccaggg ggaggaccgt atcggtcaga aaggcgaggt
     3181 agtcgccggc gcgcgtatcg gcgggccagg ggttgtactc ctgcaagcgc cgcgcgccgc
     3241 cgttctcgac tccgaccacg atcgcctcca gctccggctc cagcgccagg agggtctcgt
     3301 cgacgcgcca ttcttcgccc agaaagctgg tggcctcgtc gaagaggttc tggccgtcgt
     3361 gcatcacgat cacggggtag gcgcgctcgg agtcgaaata ggagctgggc agccgcagcc
     3421 agagcgtgcg ccggtcgccg agttgggggg catcgagatc gcgcctgagc ttgaccgtgc
     3481 cggcgacggt gtgctggccg ggcggcagcg cgttccacca ctccacgaag cccagctcct
     3541 cgaaggtcgg gccagcctgg cccggcgccg ccggctcgga gcgcagcgag gttccaacca
     3601 caaccacaac cgccctactc tctagctgtc ctaccaggct ccgcgggtga cgccgcggct
     3661 ccccggcgtg ccgccgagag ccgtaccgcg ttggctgcga ggtttagcgg cgattgaagc
     3721 agaaagcgct cgccagcgcc agcgtccgac gcacctacgc acgattctgg ccgcgatcgg
     3781 tgcgggacga tcgtcacgtt tacggcgtgg ctggcccggt cggcccgggt ggtctagagt
     3841 agggcgacaa gcgagaagct ggagccgaaa ggggaggctg atggaagaat tggttcgccg
     3901 gctcaacggg ctctgcgatg agctgccgtt ccacaccggc tggtacctca aggacctgcg
     3961 cagcggggcc gaggctgacc gcgccggcgc tctcgtcggc ccttcggcca gcacgcgcaa
     4021 ggtggcggtg ctgatgaccg cgctcaagca ggtccacgag gggaagctct cgctcgatca
     4081 gccggtgacg attcaagaga agtaccagca caacaactcc ggctgcttcc agtttctgca
     4141 acctggcttc accatgccgt ttcgagatct cttgatcatg atgatcatcg tctcggacaa
     4201 cacctgcact ggcacgatcg tcgacatggt cggcctcgat gcgatcaatg cgttttcccg
     4261 cgacgccggc atggtcggca cgacccaccg gcagaacatc ccgcccggtg ggctggcctg
     4321 ggatcacccg gtcgaggcga ccaacgccac cacgccgaag gatgtggggc tcttgctcga
     4381 ccagatcctg cagggggcag gcgataccca ggcggccgcg cgcctcggca gcacgcccga
     4441 gctctgccgc tacgccatcg agatcatgac ctggcagcag atcaccaatc gcttcccgat
     4501 gttcatgccg ccagaggcga agatcgcgca caagaccggc aagggcgtgc gcaatttcag
     4561 cgatgtcggc atcatcttcc gcggcaacga gccgcgctac atcctgaccg cctacaccga
     4621 ccgcgtgccg ccggtgctgg aggacggccg cgcgggccgc ggcgccgcct acctgctgat
     4681 cgcccagctc tgccgtgcct gctgggacgc gctagggagc taggagctgt ctgccaaaca
     4741 cgttgggcgc tgatcgagga cggatccttc gactgcgctc agggtgacat gaatggggga
     4801 ttgaggcgta cggtacgttc cgtcgtagtc gtccgcccct tcctctcccc cttgtcatcc
     4861 tgagcttgtc gaaggatccg tccccacccg gcacccgtac tgctcgccag ccaccggctt
     4921 gcgcccttcc ccggcttggg gcggaagccg accacccgcg gcagtcgtcc ggttcctcgg
     4981 gccgcgctta gcgcgcccaa ccaccagcgg cagtcggccg tgcccggcgc attcccggca
     5041 gcggccttgg gggctggcat cgcgagtgcg cggtcaaccg gcgttgaccg ccgtggacgg
     5101 tgctggtcga ggcggggggt gagtcgttga tttacggacg agacttctgg ccgctggcgg
     5161 tgggctggcg cctggtcgcg gcgccgtcct ggtcgcggga gctggagttg ctggaccagc
     5221 cgggtcagcc gccggagctg ctacgcggca acctgcgcga cctagcgcgg gtcaaccggc
     5281 ggctgggcgg agtacggctg acgcggcggg cgatcgagtc actggctgcc ggccgccacg
     5341 atacggccgc gccgctgagc gtgctggatg tcgccagtgg cgggctcgac atcccacgcg
     5401 cgctgctgac ggggggcgcg tcgctgcgcc tggtggccag cgacctgagc gccgcgatcc
     5461 tggcgctcga cgacggcgag attccacggg tcggggccga cgggcgcaac ctccccttca
     5521 tggacgacag cttcgacatc gccatctgct cgctggtgct ccatcacctg gcgccggacg
     5581 gggcgctgcg catgctgcgc gagatggcgc gagtcagccg cctggggatc gtggtcaacg
     5641 acctggtgcg ctcctgggca ggcttgctcg gcgccttcgg cctcggctgc ctcactcgga
     5701 acccgctgac ccggcacgac gcgccgctct cggtccaccg cgcctacacc cgcccccagc
     5761 tcgacgtgct gctgcgcggc gccgggctgg agccggtcgc cgcgcaccgc ttcctcggct
     5821 accgcgtggc gctggcggcg cggagccgcg cgtgacgtgc caggccgacg tgatcgtggt
     5881 gggcgccggg ccggccggcg cggcgctgac catcgggctg gcgcggcgtg ggcgcgaggt
     5941 gctgctgctc gatcaggcgg ccttcccgcg cgacaaggcc tgcggcgagt tctacagccc
     6001 tggcgtagcc gccgcgctcg cgcgcctggg cctgctcgac acgatcctcg ctcaggagca
     6061 ccggcggcat gccggcatga cgatcgtgcg gggagaggac cgcttcgccc tacgctacgc
     6121 gccgccccgg cgtgggttga gcatccggcg acgcctgctc gatcagcttc tggtagacga
     6181 ggcagcggcg agcggctcgg tggtgcgcca gcgggcacgt gtgagcggag tgctgtggga
     6241 ggacggccgg gcgaccggcg tgcgcctgcg cggcggcgag gagctgcgcg cgtcgttcgt
     6301 ggccggcgcc gacggccacc actcgacggt cggccgcgcg ctggggctgg agcgcccgcc
     6361 gcgctggccg cgccgcctcg gcctgatcgc gcacttccgg ctgaatcccg gcgcgctgcc
     6421 ggcagccgag atgcatgtcg cgggccgctt ctactgcggg ctcaacccgg tgggcgaggg
     6481 gctggtcaac gtggggctgg tggggccgct gggcacccgg cggcggggcg agaccgcggc
     6541 ccagcacctg gagcggttgc tggatgcact ccccaccgcg cgcgagcggt tggcgggggc
     6601 ggtctgggac ggcacggtgc gcggcatcgg accgctggcg caccacgc
//
LOCUS       DASUBI010000809         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_683223, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000809 DASUBI010000000
VERSION     DASUBI010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683223"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VFU72_15900"
     CDS             complement(<1..1526)
                     /locus_tag="VFU72_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEU5425025.1"
                     /translation="MSAPNRETSAARAFHDATKYVAVKDESGDEHSVMGTPPNVEPSI
                     WQEDWSLEPFAFKIYETLEPIPLPRDFAPSAMPALEAIARTGAEPSGERVPALADLAR
                     IGRLANGLLNRQTTSRSGQTIEYRTAGGTGARYHLELYFACADLPDLAAGIYHYAAHD
                     HSLRQLRRGDYRQALLAATGDEPAIARAPVVMALTSTFWRNAWRYKGRAYRHTFWDSG
                     TTLANALAVAASLELPTRLVLGYADRQVNDLLGIDGAREATVALCALGRVEQPPPPAP
                     TITPLDYPTRAISPREVEFPVIGMLHGASALASGAEVAAWRSDPLRRRPAAPTGPETA
                     LQPLGDADLPPLRIEDVILKRRSTRHYDTETRIPFSAFSTLLDRSSRGFAADCLAPGA
                     PPLHEQYLIVNGVEGLEPGVYRHEPTRHAVELLRSGEFRRQAQRLAVNQEYAANAHVN
                     AYYLTNLDPVLARYGNRGYRLAQLEPAIYAGKLHLGTHALGLGAVGSTSFDDEVTEFF
                     S"
     gene            1692..2447
                     /locus_tag="VFU72_15905"
     CDS             1692..2447
                     /locus_tag="VFU72_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5425026.1"
                     /translation="MDVTVLGTGSPNANRVTSGLVVSAPGSPPLVIDTCSGFEFFHQL
                     TRTGFDAADMRHIVLTHRHLDHIGGMAALFIHEHAFDLYALDDTHAAVEGLMAAGFPE
                     WPINPGIRRLTVRPGERHEIAGYRVSFYEVVHRVPTVAVRVEHGGKVLAYSADSVPCP
                     AVVECAREADLFLCDACYVAGDGPAMIERAKLRMHPTAWEAGQMAQEANARSLVLLHL
                     SRFATPERVLAEARERYPGPAVVAEDGGRYHVE"
     gene            2474..>2661
                     /locus_tag="VFU72_15910"
     CDS             2474..>2661
                     /locus_tag="VFU72_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HEU5425027.1"
                     /translation="MTIDTTRQTELAAPPQRGEQIPAFSGTTADGRTLGPRDFSMRRN
                     LALVFTHGPECPHCRALLR"
BASE COUNT          386 a          911 c          988 g          376 t
ORIGIN      
        1 gagaagaact cggtcacctc gtcgtcgaag gaggtcgagc cgaccgcgcc cagccccagc
       61 gcgtgcgtgc cgagatggag cttgccggcg tagatcgccg gctcgagctg cgccaaccgg
      121 tagccgcggt tgccgtagcg cgccagcacc gggtccaagt tggtcaggta gtacgcgttg
      181 acgtgcgcgt tggcggcgta ttcctggttg accgccagcc gctgagcctg ccgccggaac
      241 tcgcccgagc gcaagagttc gacagcgtgg cgcgtcggct cgtggcggta gacgcccggc
      301 tccagcccct cgacgccgtt gacgatcagg tattgctcgt gcagcggcgg ggcgccgggc
      361 gccaggcagt cggccgcgaa gccgcgcgag gagcggtcga gcagcgtcga gaaggcgctg
      421 aaggggatgc gcgtctcggt gtcgtagtgc cgggtcgagc ggcgcttcag gatcacgtcc
      481 tcgatgcgca gcggcggcaa gtcggcatcg ccgagcggct gaagggccgt ctccgggccg
      541 gtgggcgcgg ccggccggcg gcgcaacgga tcggagcgcc aggcggccac ctcggcgccg
      601 gaggcgagcg cggaggcccc gtgcagcatt ccgatcaccg gaaactcgac ctcgcgcggc
      661 gagatggcgc gggtgggata gtcgagcggc gtgatcgtgg gcgcgggcgg cggcggctgc
      721 tcgacccgcc ccagcgcgca gagcgccacg gtcgcctcgc gcgccccgtc gatcccgagc
      781 agatcgttga cctgccggtc ggcgtagccg agcacgaggc gggtgggcag ctcgagcgag
      841 gcggcgacgg ccagcgcgtt ggccagcgtc gtgccgctgt cccagaaggt gtggcggtag
      901 gcacggccct tgtagcgcca ggcgttgcgc cagaaggtgc tggtcagcgc catgacgacc
      961 ggcgcccgcg cgatggccgg ctcgtcgccg gtcgccgcca gcagcgcctg gcggtagtcg
     1021 ccgcgccgca gctggcgcaa gctatggtcg tgggcagcgt agtggtagat accggccgcg
     1081 agatcgggca ggtcggcgca ggcgaagtag agctccaggt ggtagcgcgc cccggtgccg
     1141 ccggcggtgc ggtactcgat cgtctgcccg ctgcgcgagg tggtctgacg attgagcagc
     1201 ccattggcca gccgcccgat gcgcgccagg tcggccaggg ccggcacgcg ctcgccggat
     1261 ggctcggcgc cggtgcgcgc gatcgcctcc aacgccggca tggccgaggg cgcgaagtcg
     1321 cgcggcagcg ggatcggctc cagcgtctcg tagatcttga aggcgaacgg ctccagcgac
     1381 cagtcctcct gccagatgga gggctcgacg ttgggcggcg tgcccatcac agagtgctca
     1441 tcgccggact cgtccttgac ggcgacgtac ttggtggcgt cgtggaacgc gcgggccgcg
     1501 ctcgtctctc gattgggtgc ggacactgct ctctccctac ctcgctccgt ggcaccagat
     1561 catgtcgagc gcatcatagc gtgctgcgcg ggtggcagga cgcgcggaat gcccacacgg
     1621 cagtatttcg gcagtccccg tgtaagatgg tccggcgcat gcgtgaggca ggtgaaggga
     1681 gagggggcag gatggacgtg acggtgctgg ggacaggatc gccgaacgcg aatcgggtga
     1741 cgagcgggct ggtcgtcagc gcgcccggta gcccgccgct ggtgatcgac acctgcagcg
     1801 gcttcgagtt cttccaccag ctcacccgta ccgggttcga cgccgccgac atgcggcaca
     1861 tcgtgctgac ccaccgccac ctcgaccaca tcggcggcat ggctgcgctc ttcatccacg
     1921 agcacgcctt cgacctctac gcgctcgacg acacccacgc ggcggtggag ggcctgatgg
     1981 cggccggctt ccccgagtgg ccgatcaacc caggtatccg gcggctgacg gtccgcccgg
     2041 gcgagcggca cgagatcgcc ggctaccggg tctccttcta cgaggtggtc caccgggtgc
     2101 cgaccgtggc ggtgcgcgtc gagcacggcg gcaaggtgct ggcctacagc gccgacagcg
     2161 tcccctgccc ggcggtggtc gagtgcgccc gcgaggccga cctcttcctc tgcgacgcct
     2221 gctatgtcgc cggcgacggc cccgcgatga tcgagcgggc gaaactgcgc atgcacccaa
     2281 cggcctggga ggcggggcag atggcccagg aggccaacgc gcgcagcctg gtactgctgc
     2341 acctgtcgcg ttttgctacc cccgagcgtg tcctggccga ggcgcgcgag cgctaccccg
     2401 gcccggcggt cgtcgcggag gacggcggcc gctaccacgt ggaatgagaa ccaaccgaaa
     2461 ggagctctgc ggcatgacca tcgacaccac ccggcaaacc gagctggccg cgccaccgca
     2521 gcgcggcgag cagatcccag cattctctgg cacgaccgcg gacggccgca cgctgggccc
     2581 gcgtgacttc tccatgcgcc gcaacctggc gctcgtcttc acccacggac cggagtgccc
     2641 gcattgccgg gcgctgctcc g
//
LOCUS       DASUBI010000810         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1070516, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000810 DASUBI010000000
VERSION     DASUBI010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070516"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2530)
                     /locus_tag="VFU72_15915"
     CDS             complement(<1..2530)
                     /locus_tag="VFU72_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5425028.1"
                     /translation="MAGSDPTCGELLRRYRLLAGLTQEELAERSAYSTDYISKLERDQ
                     RRPPLDAVNRLAVALGLSDQAREDLRAARVRQDTPGTSAESRGPVGRERELAEIRRQL
                     SGLGSRVLLLAGEPGIGKTRLLAAAAIGGAQSGWRVIQGGCRRSAQDPYAPFTDALAR
                     SIQGLPPLDRATVLRRAGELRALLPELADEDDGGAGFTGALHGPERPKLEPQQERRLL
                     FAAVARYLHDVAGEAGTLLVLDDLQWAGPDALDLLASLAGSPDLSIRIVGAYRDTEPT
                     NAPLGGLVADLARASLVRVLNLGPLSESEATELLSELVPDDRRLRALLPSIVRRTGGV
                     PFFLVSYAEDLQLQAGHEMRRELPWTVEQVIRQRVVALPESVQRLLAVAALVGRIVPP
                     TLLRQVIGLTEEVVLTDLETAAAARLLAEEGEDSYRFTHDLIRETIEQNLSLGRRRLL
                     HRRIGEALEQDQRTPVATLAFHFAGSDDDGKAMRYLALAGDEARQRAAHAAAADYYRE
                     ALGYSEHVNPANTAALAEKLGVELYLSGRYDESIAALERALEHTQAGDEESMRRVSGR
                     VADVHFRRGTGREVHDRVAALLTDAGTGDASLGGNLGSALRQAFARLLFEKGTFKQLL
                     AFGRSLKRLGRASRNRRLLIFGERAEGTALIQLGRLAEGVAILEPALSRGLAAGEHDR
                     MLEVAFVLTSAYLSMGAVERGLAISRQMLSQAEVLNDPVITAGHFTFLGVGFHLSGDW
                     AEARSYFQRADALFAAAAPSSIAARLANLRAHTLIWEGRWDEAQRRLESAQRAVHAMG
                     FRNAERRARTLLAELDLYRGRSQAAIDRLAPFAHADNLNDSAVHH"
     gene            2761..3111
                     /locus_tag="VFU72_15920"
     CDS             2761..3111
                     /locus_tag="VFU72_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEU5425029.1"
                     /translation="MPLFVVQHEHLPERCPAAFESGSALLAHISAATAARYGVAIQAE
                     AVLDGEHRLLLVLEAASREQVERYVRFFQRFGSVEVWAASGSELAVARGGCRAGHAVD
                     ASAQQLESTRGGDG"
     gene            3252..>3611
                     /locus_tag="VFU72_15925"
     CDS             3252..>3611
                     /locus_tag="VFU72_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425030.1"
                     /translation="MNSRKLFSLMAALALLALAPLAGFPPAAQASTTVTVCPLFCAYS
                     QIQPAIDAASNGVTIVIAAGTFSGGIIVNKNVTLQGAGATATTISGGDPVVNVDAGVT
                     ASIDGVTITGHNSDAGWG"
BASE COUNT          563 a         1262 c         1236 g          550 t
ORIGIN      
        1 ggtggtggac ggcgctgtca ttcaggttgt cggcgtgagc gaacggcgcc agccggtcaa
       61 tcgcggcctg cgaccggccg cgatagagat ccagctcggc cagcagggtg cgcgcgcggc
      121 gctcggcgtt tctaaacccc atggcgtgga cggcccgctg agctgattcc aaccggcgtt
      181 gcgcctcgtc ccagcgcccc tcccagatga gggtatgcgc gcggagattg gcgagccgcg
      241 ccgcgatcga ggatggcgcc gcggccgcga agagcgcgtc ggctcgctgg aaatagctgc
      301 gcgcttcggc ccaatcgccg gagaggtgga aaccgacgcc caggaacgtg aagtgaccgg
      361 cggtgatgac cggatcgttc aagacctcag cctgggagag catctgccgg ctgattgcca
      421 ggccgcgttc gacggcgccc atgctcaggt aggcggacgt caacacgaac gcgacctcaa
      481 gcatccggtc gtgctctcct gcggcaagcc cccgcgacag cgctggttcc aggatcgcca
      541 ccccttcggc gagccggccc agttggatca gcgccgtacc ttcggcgcgc tcgccgaaga
      601 tcagtagcct ccggttcctg ctggcccgtc ccagccgttt cagcgaccgg ccgaaggcca
      661 gcaactgctt gaacgtgccc ttttcaaaca ggaggcgagc gaacgcttgc cgcagcgccg
      721 agccgaggtt gccaccaagg ctcgcatcgc ccgttcccgc gtccgtgagc agcgccgcca
      781 cccgatcgtg cacctcgcgg ccggtgccac ggcggaaatg gacatccgcc acgcggccgg
      841 acacgcggcg catactctcc tcgtcgccag cctgagtgtg ctccagcgcg cgctccagcg
      901 cggcgatgga ctcgtcgtac cggccggaga gatagagctc cacgcccagc ttctcggcca
      961 gcgcggcggt gttcgccggg ttgacgtgtt cgctgtagcc cagcgcctca cggtagtagt
     1021 ccgccgcggc ggcgtgggct gcgcgctgcc gcgcctcgtc gccggccaac gcgagatagc
     1081 gcatggcctt gccgtcgtcg tcgctccccg cgaagtggaa ggcgagcgtg gcgaccgggg
     1141 tccgctggtc ctgctcgagc gcctcgccga tgcgtcggtg caatagccgc cgccgtccca
     1201 gcgagagatt ttgctcgatc gtctcgcgga tcaggtcgtg ggtgaagcgg tagctgtctt
     1261 ccccttcctc cgccagcagt cgcgcggccg ccgccgtctc gaggtcagtc agcaccacct
     1321 cctcggtcag cccgatgacc tggcgcagca gcgttggggg cacgatccgc ccgaccaggg
     1381 cggccaccgc taacagtcgc tgcaccgatt ccggcagggc caccacccgc tgccggatga
     1441 cctgctccac ggtccacggc agctctcgtc gcatctcgtg gcccgcctgc agttggaggt
     1501 cctcggcata gctgaccagg aagaagggga ccccgccggt ccgccgcacg atggacggca
     1561 ggagcgctcg gagccgcctg tcgtctggaa ccagttcgga gagcagctcg gtggcctccg
     1621 actcggagag cgggccgagg ttgaggacgc gcaccagtga ggcgcgggcc aggtcggcga
     1681 ccaggccgcc gaggggggcg ttcgtcggct cggtgtctcg ataggcgccg acgatccgga
     1741 tggacaggtc gggggagccg gcgagcgacg ccaggaggtc cagggcatcc ggaccggccc
     1801 actgcaggtc gtcgaggacc aggagtgtgc cggcctcacc agccacgtcg tgaaggtagc
     1861 gagccaccgc ggcaaagagg agacggcgct cctgttgcgg ctcgagcttc ggccgctccg
     1921 gcccatgaag agcccccgta aacccggcgc ctccgtcatc ctcgtcggcc agctccggga
     1981 gcagcgctcg gagctcgccg gcacggcgca gcacggtcgc gcggtcgagt ggtggcagac
     2041 cctgaatcga gcgggcgagc gcgtcagtga acggggcgta cggatcctgc gccgagcgac
     2101 ggcagccgcc ctggatcacc cgccagccgc tctgagcccc cccgatcgcc gccgccgcca
     2161 ggaggcgcgt cttgccgatg cccggttccc ccgcgagcag gaggacgcgc gacccaagcc
     2221 cgctcaactg gcggcgaatc tcggccagct cgcgctcgcg gccaaccggc ccacgacttt
     2281 ccgcactcgt cccgggcgtg tcctgccgca cacgcgccgc acggagatcc tcccgcgcct
     2341 ggtcactcaa gcccagggcc acggccaggc gattgacggc atcgagcggc ggccggcgct
     2401 gatcacgctc cagcttgctg atgtagtcgg tgctgtaggc gctgcgctcg gccaactcct
     2461 cctgcgtgag gccggccagc aggcggtagc gccggagcag ctccccacag gtcggatccg
     2521 atccggccac acctgctcct tgcctccggg taggaactgg agccgaaccc ggaaagtgtc
     2581 acgtcggtct ctctctacat tacgttcacc cacaccggtt gtcaacgccg tgtgcgcgat
     2641 ttgtccgggt cgtttgtccg agtctgtctg tggtcaaggc agccggcgat gcacagactg
     2701 agatgcagac agcagcgcac gacggccgaa tttcggccgc gggtcggaaa ggagacggac
     2761 gtgcctctct ttgtggtcca gcacgagcac ctgcccgagc gctgccccgc cgcgtttgag
     2821 tcgggctccg cgctcttggc gcacatctcg gccgctaccg cggcgcgcta cggcgtggcc
     2881 atccaagccg aggcggtgct cgacggcgag caccggctgc tgctggtgct ggaagcagcg
     2941 agccgcgagc aggttgagcg ctacgtgcgc ttcttccagc gcttcggcag cgtcgaggtg
     3001 tgggccgcct cgggcagcga gctggccgtg gcccgcggcg gctgccgcgc gggccatgcc
     3061 gtcgatgcca gcgctcagca gctcgagagc acgcgcggag gtgacgggta acctgacgca
     3121 gcgccaggga catggaggga ttgtcatcga tgctttgcgc ctccgtggta ggcgacaaag
     3181 gctcactgcc gaaatcggca attcgtcacg acatgaagac atgacatacg tattcacacg
     3241 ggagagcggg catgaattcc agaaaactct tcagcctcat ggcggcgctg gcgctgctgg
     3301 cgctggcgcc gctggccggc ttccccccgg cagcccaggc atccaccacc gtcactgtct
     3361 gccccctttt ctgcgcctac tcgcagatcc agccggccat cgatgccgct tccaacggcg
     3421 tcaccatcgt catcgcggcg ggcaccttta gtggcggcat catcgtcaac aagaacgtca
     3481 cgctgcaagg ggcgggggcg acggcgacga cgatctccgg gggtgatccg gtggtgaatg
     3541 tcgacgccgg ggtcaccgcg agcatcgacg gcgtgacgat caccggccac aacagtgatg
     3601 ccggttgggg c
//
LOCUS       DASUBI010000811         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1165789, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000811 DASUBI010000000
VERSION     DASUBI010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165789"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFU72_15930"
     CDS             <1..364
                     /locus_tag="VFU72_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5425031.1"
                     /translation="AGREIGLWTAHAGVDPRNVDRAIESILAEIEKLRSEGVTARELE
                     DAKSYLVGSLPLGLESSDAIAGTILDLIFYGLGLDYIERLPDRIRGLTAEELRAAAER
                     YILPDQLAIAVSLPAAAE"
     gene            461..1132
                     /locus_tag="VFU72_15935"
     CDS             461..1132
                     /locus_tag="VFU72_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5425032.1"
                     /translation="MAGTTAYTVFWIYQATPEWRRQSEQAKQNARQEFIAALEDSGDV
                     GVRGVYSTVGFRADADLIVWSVAEDADALQRFAVRLRQTALGALLSQTQAYLGVAGGS
                     QYDPSHAPAFLKGTPPRRYLSVYPFTKTPEWFLLPFEERRRLMRVHGELGQEYPTILT
                     NTVNSFGIQDQEFIVALEDDDPDVLVQMVQRLRGAEVRRYTQVDTPIYLGQLKAPDEA
                     LGQLG"
     gene            complement(1095..1925)
                     /locus_tag="VFU72_15940"
     CDS             complement(1095..1925)
                     /locus_tag="VFU72_15940"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEU5425033.1"
                     /translation="MPPTEDANSSLTPTRLTAGVILAGGIALAGWRAGALTGGGALAA
                     VGVGAAVFGAGGRRFVPPMLGFFGLSSALSHLGTERKRALAEVAAKGERRDAGQVLAN
                     GGVPALLALASAAGGRDVFSGYLGALAAVNADTWSTELGGLSRRAPRSLATGKLVASG
                     TSGGVTALGLAGALAGGAVVGALGRRTWRSVLLGTLAGLAGSLVDSLLGATVQRVSWC
                     PVCGKETERAVHGCGTPTEYRRGYPWLDNDRVNLLASLSGALAGCALSRAGRAPRRAP
                     "
     gene            complement(2004..2249)
                     /locus_tag="VFU72_15945"
     CDS             complement(2004..2249)
                     /locus_tag="VFU72_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425034.1"
                     /translation="MTLDEATATRIVEEAVEEAGGRRRIHGSPRHPFALNPTREVEIE
                     GHTVVIRYGEASSPAIAELEGYIFDIRPEGLILLFGP"
BASE COUNT          358 a          875 c          786 g          305 t
ORIGIN      
        1 ggccgggcgc gagatcgggc tctggacggc acatgccggg gtggacccgc gcaacgtcga
       61 ccgcgcgatc gagagcatcc tggccgagat cgagaagctg cggagcgaag gcgtcaccgc
      121 gcgcgagctg gaggacgcca agagctatct ggtgggcagc ctgccgctgg ggctggagag
      181 ctccgacgcc atcgccggta cgatcctcga cctgatcttc tacgggttgg gccttgacta
      241 catcgagcgg ctgccggatc gcatccgcgg cctgaccgcc gaggagttgc gcgccgccgc
      301 cgaacgctac attctgcccg accagctggc gatcgcggtc agcctgccgg cggcggccga
      361 gtgaggaacg cgccccgagc ctgccggcgg gtggcataat cgccttgagc gcgcgccgct
      421 tgaccgcgcc ggagtcgaga gcggaggagc agagaaggtc atggctggca ccactgccta
      481 cacagttttc tggatctacc aggcgacgcc cgagtggcgc cggcaatccg agcaagccaa
      541 gcagaacgcc cgacaggagt tcatcgcggc gctggaggac tccggcgacg tcggtgtgcg
      601 cggggtctac tcgaccgtcg gctttcgcgc cgacgccgac ctgatcgtct ggtcggtggc
      661 cgaggacgcc gacgccctgc agcgcttcgc ggtccgcctg cgccaaaccg cgctcggcgc
      721 gctgctcagc cagacgcagg cctatctcgg cgtcgccggc ggctcgcagt acgacccgag
      781 ccacgcgccg gcctttctca agggcacgcc gccgcggcgc tacctcagcg tctacccctt
      841 caccaagacg cccgagtggt tccttctccc cttcgaagag cgccgccggc tgatgcgcgt
      901 ccacggcgaa ctgggccagg agtacccgac gatcctgacc aatacggtca actccttcgg
      961 catccaggac caggaattca tcgtggcgct ggaggacgac gatcccgacg tgctggtgca
     1021 gatggtgcag cgcctgcgcg gcgcggaggt acgtcgctac acccaggtgg atacgccgat
     1081 ctatctgggc cagctcaagg cgcccgacga ggcgctcggc cagctcggct gagcgcgcag
     1141 ccggccagcg cgccgctgag cgaagccaac aggttgaccc ggtcgttgtc gagccagggg
     1201 tagccacgcc ggtactcggt cggcgtgccg cagccgtgga ccgcccgctc cgtctccttg
     1261 ccacagaccg ggcaccacga cacgcgctgc acggtggcgc cgaggaggct gtcgaccagc
     1321 gagcctgcca gccccgccag cgtgcccagc aggaccgacc tccaggtacg gcggcccagg
     1381 gcgccgacga cggcgcctcc ggccagcgcg ccggccagcc cgagcgcggt cacaccgccc
     1441 gaggtgccgg acgccacgag cttgccggtc gcgagcgagc gcggcgcccg cctactcagg
     1501 ccgcccagct cggtggacca ggtgtcggcg ttgaccgccg ccagcgcgcc gaggtagccg
     1561 gagaagacgt cacgcccgcc ggcggcgctc gccagcgcca gcagcgccgg cacgcccccg
     1621 ttcgccagca cctgccccgc gtcgcgccgc tcccccttgg ccgccacctc ggccagcgcc
     1681 cgcttgcgct ccgttcccag gtgcgacagc gcgctggaga gaccgaagaa gccgagcatc
     1741 ggcgggacaa agcgtcgccc gccagccccg aacaccgccg cgccaacacc gaccgccgcc
     1801 agcgccccgc cgcctgtcag cgcccccgcc cgccagcccg cgagcgcgat gccgccggcg
     1861 aggatgacac ccgcggtaag gcgcgtgggg gtgagggagg agttggcgtc ttccgtgggg
     1921 ggcatgtgga acctgagacc caagaatgga attcccgggc gcctcccgcg cgaccgctcc
     1981 acagttcgtg caagactcac cccctacggc ccaaacagca agatcaaccc ctccggccgg
     2041 atgtcgaaga tgtagccctc cagctcagcg atcgccgggc tgctcgcctc gccgtagcgg
     2101 atgacgacgg tgtggccctc gatctcgact tcgcgcgtcg ggttcagcgc gaaggggtga
     2161 cgcgggctgc cgtggatgcg gcgccgcccg cccgcctcct ccaccgcttc ctccacgatc
     2221 cgggtcgcgg tcgcctcgtc cagcgtcatt cattgtcctc ccggctccgc tcgccgccgc
     2281 gataaggcgc cacacgccgc ggcacacgcg tatcttagcg gttc
//
LOCUS       DASUBI010000812         7989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_961682, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000812 DASUBI010000000
VERSION     DASUBI010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961682"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFU72_15950"
     CDS             complement(<1..271)
                     /locus_tag="VFU72_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5425035.1"
                     /translation="MPATTLIPAAVEAANQSFGQFSLPAATIRALAGMDIEQPTPIQA
                     AVLPLLLEGRDVIAQARTGSGKTLAFLIPALEMLDPARREVQVLVL"
     gene            619..855
                     /locus_tag="VFU72_15955"
     CDS             619..855
                     /locus_tag="VFU72_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5425036.1"
                     /translation="MATGHIKTLVEDRGFGFIAVDDASSPHDVFFHHSAVEGGNFEGL
                     REGQQVEFDEQPDPRNPQRTRAAHVRVSEESSSF"
     gene            complement(968..1315)
                     /locus_tag="VFU72_15960"
     CDS             complement(968..1315)
                     /locus_tag="VFU72_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU5425037.1"
                     /translation="MSFVDKTLTCRDCGQPFTFTAGEQEFYAEKGFTNQPSRCPDCRR
                     AAKAARNGGGSSSYSSGGGYGAPRQERTMYPATCSDCGKETMVPFMPRNDKPVYCSDC
                     FQAHRQERSYNRW"
     gene            complement(1548..1925)
                     /locus_tag="VFU72_15965"
     CDS             complement(1548..1925)
                     /locus_tag="VFU72_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425038.1"
                     /translation="MGYLPAFTHPELAASPSARYYEDLLEQSAAARSNGDHLQAQRLA
                     SAAHKWAMDEIIRLQRVRQAEAELAQATKQLESAWDRDRACITKEELGERHPEFRRLI
                     TRLETAEARLSDLSVAIPADVSG"
     gene            complement(1991..2212)
                     /locus_tag="VFU72_15970"
     CDS             complement(1991..2212)
                     /locus_tag="VFU72_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425039.1"
                     /translation="MYDRPRAIKIVLPDGELLTLLGAEVRFEPFYPESIRVSLPSVEA
                     EAIALVCLNTRGEEVARIKMNAIIGYLTE"
     gene            2825..3010
                     /locus_tag="VFU72_15975"
     CDS             2825..3010
                     /locus_tag="VFU72_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425040.1"
                     /translation="MRFQFERSFGNEQFGRSRLQHGYRRGNECADCRRDQRGECGRLP
                     GVDGGQRPRGGQRPRGD"
     gene            3000..3785
                     /locus_tag="VFU72_15980"
     CDS             3000..3785
                     /locus_tag="VFU72_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5425041.1"
                     /translation="MATSAPPSGKKGGDIALAQSSDATSLDPAKSTATVDSQIYLAVY
                     DHLITLDANGALAPELAESWEEAPDHLSWTLRLRKGIKFHDGTDFTAAAVKTNIERYI
                     DPALNSPRKGEIAAVTGVDAVDDSTAKIKLSQPFVTLPYALTGSSGVVVSPAAIQKYG
                     DDLARNPVGTGPFLFKEWVKNDRVLVARNDSYWKTGRPLLNTVSYKGISDSSVILNGM
                     KSNALQVTYGIAAKDVASVKDDPTFQLILKPSTSISSLRLNDA"
     gene            3778..4485
                     /locus_tag="VFU72_15985"
     CDS             3778..4485
                     /locus_tag="VFU72_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5425042.1"
                     /translation="MRSRPSIRRRSVKKKEIRQAVSWAIDRAAIEKALYFDTGVPANT
                     FLGPLNLGYNKDITLYKTVDTAKAKELLAAGGQPNGFKFSAQTTNNTDALKLAQAIKS
                     QLEAINVDMELELVDGVTLLQKLTQKQFQATFVTYNGGVEPFQGFSRFFLSTSTLNVY
                     GYNNPQYDQLLTQAGGTTDNDARAKIYSQLTAILSEDCPWVFLRYPAQTELFRKELKG
                     YVYIPDGGIRLDTVSLA"
     gene            4519..5463
                     /locus_tag="VFU72_15990"
     CDS             4519..5463
                     /locus_tag="VFU72_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5425043.1"
                     /translation="MLAFIIKRLLTTIPTILLVTVVVFGILRITPGGPAVAMLGEQAS
                     PEAVAALRHKLGLDQPLYRQYLHWLGGALHGDLGRSAFGNQPVTELIVQRIMPTAELG
                     FLALLISLLIGLTLGIVAAVRHNSLSDAISSFIAILGVSTPSFWLAILLVLIFAVKLH
                     WLPALGYVSPLADPLTNLKQMILPSITLGVILAAVVTRFTRAAMLDALYQDYVRTARA
                     KGLKERRVVVRHALSNALIPIVTVVGLQLGGVLSGAVIIETIFALPGNGQLLVNAIFS
                     RDFPVVQGLVVVIAIVFIAVNVAVDILYGLLDPRIRLS"
     gene            5477..6382
                     /locus_tag="VFU72_15995"
     CDS             5477..6382
                     /locus_tag="VFU72_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5425044.1"
                     /translation="MSEQTALKGSSVDRGGRLRGGPEPYWRWVVKRIIRVRLAPLSLA
                     IVVLSVLMALFAGQLAPDSPTKSQLTQVLERPSSTHVMGTDDLGRDVLSRIIFGSRAS
                     LSAGVVSTGIGLALGTFLGLISGYFLGIADTVIMRVMDALLAFPGLILALAITAALGP
                     SLINAMIAIGIVSVPRFARLARGQVLSLRQTEYVEAVRALGAGNNRILFRHIGPNMLT
                     PIIVQASLSVAFAILTEASLSVLGLGVQPPTPSWGFMLNQGRDYLGQAPWLAIFPGIA
                     IFLVVMAFNLLGDAIRDALDPRLRL"
     gene            6458..6970
                     /locus_tag="VFU72_16000"
     CDS             6458..6970
                     /locus_tag="VFU72_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU5425045.1"
                     /translation="MATETLPAGALPPHARSPRESEVLLAQMMLPTDANPYGNVHGGV
                     IMKLADTAGGLAATRHCRRRVVTVTVDSMSFLHPVFVGDLVRLRACLTWTGRTSMEAE
                     VTVEAEDVISGRVTTTSTAYLVYVALDDAGQPVPIPALKLETADDHRRWHEAEGRQAH
                     RLSVRGSKER"
     gene            6970..7815
                     /locus_tag="VFU72_16005"
     CDS             6970..7815
                     /locus_tag="VFU72_16005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5425046.1"
                     /translation="MRRLLALDIDGTLLDPSDIVTPVVRAAIAEARAQDVAVALATGR
                     RLRSTRRVVEELGIQLPLVLSNGALIWDSAAEAPLREAIFTPELIAAALEASLAAGLA
                     PILLQGPLHGERIVVPAGEPLEQYARAFGRAWLDVEAVPAASLPQTAEVLTIDLFGPH
                     RPVRAVTDGLAARGLSIFHTGPHQVGGVAHLWAANIHMSGVSKAAAVARLAADLGLTM
                     ADVVAVGDGENDLPLLEAAGIGVAMGNAAPEVRARADAVVRGHDADGVAEAIERFVLN
                     RQAAD"
BASE COUNT         1288 a         2677 c         2685 g         1339 t
ORIGIN      
        1 tcaggaccag cacctggacc tcgcgcctgg cggggtcgag catctcgagc gccgggatca
       61 ggaaggcgag tgtcttgccc gagccggtac gggcctgggc gatgacgtcg cgtccctcca
      121 gcagcagcgg tagcaccgcc gcctggatcg gggtcggttg ctcgatatcc ataccggcga
      181 gcgcccggat ggtggcggcg ggcagtgaaa actggccgaa cgattggttg gccgcctcga
      241 cggcagccgg aatgagcgtg gtggcaggca tgaaaatacc ctctgactcc gcagcgaaac
      301 ggacatagct gtgttggaac cgtcaagctg gctagtacgg ctcgacggcc cttcgctgcg
      361 agtagagggc gcattgacgt actaccgcgc ggggcaagcg acccccgcgc actccgaact
      421 ctgactaagt ataccagccg gtcagctctc ccggcgagcc cggccggggg gtgtctgggc
      481 accggacgtg caagcggaac ggaaaagtct cctgtgtgcg gaggcgcgtt actgcggagg
      541 aagccggctg gtccccctct cagtgacgag atcggctgtc gaaccccgtt cgactacgga
      601 actgtgagga tctggcgcat ggcaactggt cacatcaaga cgctcgtcga ggatcgcggc
      661 ttcggcttca tcgcggtgga cgacgcgagc agcccgcacg acgtcttctt ccaccactcg
      721 gcggtcgagg gcggcaactt cgaggggctg cgcgaggggc agcaggtgga gttcgacgag
      781 cagccggacc cgcgcaaccc acagcgcacc cgcgccgcgc acgtgcgtgt gagcgaggag
      841 agctcgagct tctagctcag cgggcgccac actctccaag agccggaaac aacaacgccc
      901 ctgggtgagg gcaacccagg ggcgtgcatt gactatgaaa gcgcgcgccg aatggcgcgc
      961 cggtctgcta ccagcggttg tagctgcgct cctgacggtg cgcctggaag cagtccgagc
     1021 agtagaccgg cttgtcattg cgcggcatga aggggaccat ggtctccttg ccgcagtcgc
     1081 tgcaggtcgc cgggtacatg gtgcgctcct ggcgcggcgc gccgtagcca ccaccactgc
     1141 tgtagctgct gctgccgccg ccgttgcggg cggccttggc cgcgcgccgg caatccgggc
     1201 agcggctggg ctgattggtg aaccccttct ccgcgtagaa ctcctgctcg ccagcggtaa
     1261 aggtgaacgg ctggccgcag tcgcggcagg tcagggtctt gtcaacgaag ctcatcaagc
     1321 gtactccttg ggaatcgata ccgctcaccc tgagcggctc gctgaatttg atcgtccagc
     1381 gcaagttttt cgacgagatg agctcggaga catgccctcg ctgtcgtccg cggcacacgc
     1441 gaaaatccag gctagctgag atcagtgtac cacaaatggc cgcgcaatgc agcacaaggc
     1501 acggtccgcg tcggacaggc gcgtgaggaa taccggggga tggccgctta gccgctcaca
     1561 tcggccggga tcgccaccga caggtcgctc aagcgcgctt cggcggtctc caggcgtgtg
     1621 atcaagcgcc ggaactcggg gtggcgctca ccgagctcct ctttggtgat acaggcgcgg
     1681 tcgcggtccc aggcgctctc gagctgcttg gtggcctggg ccagctcggc ttcggcctgc
     1741 ctgaccctct gcaggcggat gatctcatcc atggcccact tgtgcgccgc gctggccagc
     1801 cgttgcgcct gcaggtgatc gccgttgctg cgcgcggcgg ccgattgctc cagcagatcc
     1861 tcgtaatagc gcgcgcttgg cgatgcggcc agttcggggt gggtaaaggc cggcagataa
     1921 cccatgagca gctccctact agcgtcgaat gcgaggtcgt cgccgcgcgg cgttcccggt
     1981 ttctctcagg ctattccgtg agatagccga tgatggcgtt catcttgatc cgggcgacct
     2041 cttcgccgcg cgtgttgaga cagacgagcg cgatagcctc cgcctccacc gatggcagcg
     2101 atacgcggat cgactccggg tagaacggct caaaccggac ctccgcgccg agcagggtca
     2161 gcagttcgcc atcgggaagc acgatcttga tggcgcgcgg gcgatcgtac atcgtggtct
     2221 cctctgcacg gctgcctcga gcgccgcccc caccagcgaa ggggcgagtt gaccaagtgc
     2281 ggaacgcgtc gcggtcgtcg cgtggatcgt tgaaggctgc cagcggcagg cgtcctaagc
     2341 gggcccgcac gctcgatcca aaggacccgc ccggccggaa ggaggtcttc cctgcgcgct
     2401 tgctttcagt atacccggcg cggcaaggcc gctcccggct cgcgctctcc gagcacgaga
     2461 gatacgcgat tgacaatgcc tcgtgccact cctatgatgc gcgcaacgag atccatgcgg
     2521 tgtgcccccc tgggtggaga cgaatcggga attgccgagc gcgttgcccg tgttatcagc
     2581 ctcgattcgg gtcgatgtct cggcgccgcg ctcagcggtg gcccggcgcg gcttggcgtc
     2641 cgcggtgcgc gggcacaacc ggatcgccgc gggccgtcga accacgatgc gagcgctgcc
     2701 gggcagcgcc agaggaggag aacatcgtgt cggacaacgt ggtgcggggc gtgacgcgcc
     2761 gcagcctgct gcgtgggatt ggcgttgcct cggccgtcgg ggcattctct ccgctgctcg
     2821 ccgcgtgcgg ttccagttcg agcgctcctt cggcaacgag cagttcgggc ggagccggct
     2881 ccagcacggc taccgccgcg gcaacgagtg cgccgactgc cgccgcgacc agcgcggcga
     2941 gtgcgggcgc ctccccggcg tcgacggcgg ccagcgcccc cgtggcggcc agcgcccccg
     3001 tggcgactag cgccccgccg tcgggcaaga agggcggcga tatcgcgctg gcgcaaagct
     3061 cggacgcgac ctcgctggac ccggccaagt cgaccgcgac cgtcgacagt cagatctatc
     3121 tggccgtcta cgatcatctg atcacgctcg acgcgaatgg agcgctggcg ccggagctgg
     3181 ccgagtcgtg ggaagaagcg ccggatcatc tgagctggac cctcaggctg cgcaagggga
     3241 tcaagttcca cgacggcacc gatttcactg ccgccgccgt caagacgaac atcgagcgct
     3301 acatcgaccc cgcgctcaac tcgccccgca agggtgagat cgccgccgtg accggcgtcg
     3361 atgcggtcga cgactccacc gccaagatca agcttagcca gccgttcgtc acgctcccct
     3421 acgctctgac cggcagcagc ggcgtggtgg tgtcgccggc cgcgatccag aagtacggcg
     3481 acgacctggc ccgcaacccg gtcgggaccg gcccgttcct gttcaaggag tgggtcaaga
     3541 acgaccgcgt gctggtcgcg cgcaatgata gctactggaa gacggggcgc ccgctgctca
     3601 acacggtcag ctacaagggg atctccgact ccagcgtgat cctcaatggg atgaagagca
     3661 acgcgctgca ggtgacctac ggcatcgcgg ccaaggacgt ggcgagtgtc aaggatgatc
     3721 ccaccttcca gttgatcctc aagccgtcga cctcgatctc ctcgctccgt ctcaacgatg
     3781 cgtagccgcc cttcgataag aaggagatcc gtcaagaaga aggagatccg tcaagcggtc
     3841 tcctgggcta tcgaccgggc cgcgatcgag aaggcgctct atttcgacac cggcgtgccg
     3901 gccaacacgt tcctcggccc gctcaacctg ggctataaca aggacatcac gctctacaag
     3961 acggttgaca cggccaaggc caaggagttg ctggctgccg ggggtcagcc gaatgggttc
     4021 aaattcagcg cgcagacgac gaacaacacc gacgcgctca agctggccca ggccatcaaa
     4081 tcgcagctcg aggccatcaa cgtcgatatg gagctcgaac tggtcgacgg cgtgacgctg
     4141 ctgcagaagc tcacgcagaa gcagtttcag gccaccttcg ttacctacaa cggcggcgtc
     4201 gagccgttcc agggcttcag ccggttcttc ctgagcacca gcacgctcaa cgtctacggc
     4261 tacaacaacc cgcagtacga ccaactcctc acccaggcgg gcggcacgac cgacaacgac
     4321 gcgcgggcga agatctactc gcagctcacc gcgatcctct ccgaggattg cccctgggtc
     4381 ttcctccgct atccggcgca gaccgagctg ttccgcaagg agctcaaggg ctacgtctac
     4441 attccggacg gcggtatccg tctcgacacg gtctcgttgg cctgacgagc ggcccggctc
     4501 ggcgagcgga gggcggcctt gctggcattc atcatcaagc gcctgctgac gacgatcccc
     4561 accatcctgc tggtgacggt agtcgtgttc ggcattctgc gcatcacgcc gggcggcccg
     4621 gcggtggcga tgctgggtga gcaagccagc cccgaggcgg tcgccgcgct gcggcacaag
     4681 ctcggcctcg accaaccgct ctaccggcaa tacctgcact ggctcggggg ggcgctccac
     4741 ggcgacctgg ggcgctccgc gttcggcaac cagccggtga ctgagctgat cgtgcagcgc
     4801 atcatgccga cggccgagct tggctttctc gccctgctta tctccctcct gatcgggttg
     4861 acgctcggca tcgtggcagc ggtgcggcac aactcgctct cagacgcgat tagctccttc
     4921 atcgcgattc tcggagtctc gaccccatcg ttctggctgg cgattctctt ggtgctcatc
     4981 ttcgcggtca aacttcactg gctaccggcg ctcggctacg tcagcccgct ggccgatccg
     5041 ctcaccaacc tcaaacagat gattctgccg tcgatcacgc tcggggtgat tctggccgcg
     5101 gtcgtgaccc gcttcaccag ggcggcgatg ctcgacgcgc tctatcagga ctacgtgcgg
     5161 accgcgcgcg ccaagggcct gaaggagcgg cgcgtcgtgg tgcggcacgc gctgagcaac
     5221 gctctgatcc cgatcgttac ggtcgttgga ttgcaacttg gcggcgtgct cagcggcgcg
     5281 gtcattatcg agacgatctt cgcccttccc ggcaatgggc agttgctggt caacgccatc
     5341 ttctcgcgcg atttcccggt cgtgcagggt ctggtcgtgg tgatcgcaat cgtcttcatc
     5401 gcggtcaacg tcgcggtgga cattctctac ggtctactcg acccacgcat ccgcttgtca
     5461 tgagaggagc gctcgcgtga gcgagcagac agccctcaaa ggctcgagtg tcgatagggg
     5521 agggcggctg cgcggcgggc cggagccgta ctggcgctgg gtcgtcaaac gcatcatccg
     5581 ggttcgcctc gcgccgctat cgctggcgat cgtggtgttg tccgtgctga tggcgctctt
     5641 tgcggggcaa ttggcaccgg atagcccgac caagtcgcag ttgacgcagg tcttggaacg
     5701 accgtcgtcg acgcacgtga tgggtaccga cgacctcggg cgggacgtcc tgagccggat
     5761 catcttcggc tcccgcgcca gtctctcggc gggcgtcgtc tccaccggga ttgggctggc
     5821 gctcggcacc tttcttggtc tgatctccgg ttatttcctc ggcatcgccg acacggtcat
     5881 catgcgcgtc atggatgcgc tgctcgcctt ccccggcctg atcctggcac tggctatcac
     5941 cgccgcgctc ggtccgagcc tgatcaacgc catgatcgcg atcgggatcg tcagcgtgcc
     6001 gcgtttcgcg cgcctggcgc gcggtcaggt tctctcgctg cgccagaccg agtatgtcga
     6061 agccgtgcgg gcgctgggcg cggggaacaa tcgcatcctg ttccggcaca tcggaccgaa
     6121 catgctgacg ccgatcatcg tgcaggcgtc cttgagcgtg gccttcgcca tcctgaccga
     6181 ggcgagtttg tcggtcctgg ggctcggcgt gcagccgccg acgccgagct gggggttcat
     6241 gctgaaccag ggtcgcgact acctgggcca ggctccctgg ctcgcgatct ttcccggtat
     6301 cgccatcttc ctggtcgtga tggccttcaa cctgctgggc gacgcgatcc gcgacgcgct
     6361 cgatccgcgt ctgaggttgt gacgagaagg ttccccagcc ccggccgcgc cgcgccacac
     6421 ttgcacgcgc cgcacccggc gcgctaggct ctgtcgcatg gcgaccgaga cgctcccggc
     6481 cggcgcactc cccccacacg cacgctcgcc gcgcgagagc gaggtgctgc tggcgcagat
     6541 gatgctgccg accgacgcca acccctacgg caacgtccac ggcggcgtga tcatgaagct
     6601 ggccgacacc gccggcgggc tggcggctac ccggcactgt cgccggcgcg tcgtcaccgt
     6661 cacggtcgac tcgatgagct ttctccaccc ggtcttcgtc ggcgatttgg tgcggctgcg
     6721 cgcctgcctg acctggaccg gccgcaccag catggaggcg gaggtcacgg tcgaggccga
     6781 ggacgtgatc tcggggcgcg tcacgaccac ctcgacggcc tatctggtct acgtcgcgct
     6841 cgacgacgcg ggccagccgg taccgattcc cgcgctcaaa ctggagacgg cggacgatca
     6901 ccgccgctgg cacgaggccg aggggcgcca ggcgcaccgg ctgtccgtgc ggggcagtaa
     6961 ggagagatga tgcggcgctt actggccctc gacatcgacg gcacgctgct cgacccgtcg
     7021 gatatcgtca cgccggtcgt gcgggcggcg atcgccgagg cgcgcgcgca ggatgtcgcg
     7081 gtggcgctgg ccaccgggcg gcggctgcgc tcgacgcgcc gggtggtcga ggagctgggg
     7141 atccaactgc cgctggtcct ctccaacggc gcgctgatct gggattccgc cgccgaggcg
     7201 ccgctgcgcg aggcgatctt cacccccgag ctgatcgccg cggcgctgga ggcgtcgctg
     7261 gccgccgggc tggcgccgat cctcctgcag gggccgctgc acggcgagcg catcgtcgtc
     7321 ccggcgggcg agccgctgga gcagtatgcc cgcgccttcg gccgcgcctg gctcgacgtc
     7381 gaggcggtgc cggccgcctc gctgccgcag accgccgagg tgctgaccat cgacctcttc
     7441 gggccgcacc ggccggtgcg cgccgtgacc gacgggctgg cggcgcgcgg cctctcgatc
     7501 ttccacaccg ggccacatca ggtggggggc gtcgcgcacc tctgggcggc caacatccac
     7561 atgtccggcg tcagcaaggc ggcggccgtg gcgcggctgg cggcagacct cggattgacc
     7621 atggccgacg tggtggcggt cggcgacggc gagaacgacc tgccgctgct ggaggccgcc
     7681 ggcatcgggg tggcgatggg caacgccgcg ccggaggtgc gggcgcgggc cgatgcggtg
     7741 gtgcgcggtc acgacgccga cggggtggct gaggcgatcg agcgcttcgt gctcaatcgc
     7801 caggccgccg actagccgac cctcggcctc tggaccacgc aacgccgaac ccatatactc
     7861 gaacgaacag gcgttctata gcgacaccca tgcaagaaca ggggcaggcg atgcgcgcgc
     7921 gcgatttgac cacgctgtac gactactact actgggcgac gaagaagatt ctggagcagg
     7981 cggggatgg
//
LOCUS       DASUBI010000813         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1273154, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000813 DASUBI010000000
VERSION     DASUBI010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273154"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="VFU72_16010"
     CDS             <1..2058
                     /locus_tag="VFU72_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425047.1"
                     /translation="GVSASRGVGFRAERGQVLCTTIRENPRARDLIGRVRGDALATGT
                     TEHHHTARNLKGWLLQGYGHEMAGPYEREEHHETRPWWQVMCLTGLDYFSTLGYQPGI
                     AALAAGALAPIATLVLVLLTLFGALPVYRHVAEESPHGQGSIAMLERLMGWWKGKFFV
                     LALLGFAATDFMITMTLSSADATAHIVENPFTPSVLHGHTIPITLALLLILGLVFLKG
                     FAEAISIAVVLVFSYLALNLVVIGDCLWHVLQHPALVTAWRHALVVEHPSLLLMAVYA
                     VLVFPQLALGLSGFETGVAVMPLVRGDGPEGTSIDGRIKNTKKLLNTAALLMSFYLIT
                     SSFVTIVLIPSAAFQVGGAANGRALAFLAHQYLGSAFGTVYDVSTIFILWFAGASAMA
                     GLLNLVPRYLPRYGMAPEWTRAVRPLVVLFTVIAFAITLIFDASVDKQGAAYATGVLV
                     LMTSGAIAVTLSVADLRRRWLVVAFGFIAAVFIYTTIANVFERPDGVKIAAFFIGTIV
                     IMSLVSRVWRVRELRVTEIEVDPAAERFIADAAGVGTLRVIAHDPEHRWPGEYEDKLQ
                     QECEHHNIPSADVFFLEVKVEDPSDFAGRLDVHGTTVGGFRIFRIQSSAIPNAIAAFL
                     LYLRDRTGKIPNAYFDWSEAGPLVQLSRYVFFGEGDIAPVTHEVLRQAEPLPDRRPMI
                     HVG"
     gene            2308..3525
                     /locus_tag="VFU72_16015"
     CDS             2308..3525
                     /locus_tag="VFU72_16015"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5425048.1"
                     /translation="MSGNGKAKANQPVILSAARTPIGRFQGALANVPAAELGATAIRA
                     AVERAGIEGHLGEIDEVLMGNVVSAGLGQNIARQCALKAGIPSTVGAITVNKVCGSGL
                     KAVMLSAQAIRAGDGDLFVAGGVESMSRAPYLVHGRLGELRYGNAEMKDSLVSDGLWC
                     AIENWGMGNAAEFIADEYGVSREAMDQYALASHQKAVAAMDEGRFKDEIAPVTVPGRR
                     GQETVVEQDEAPRRDTSLEALSKLRPAFVAEGRVTAGNAPGLNDAAAALVIASAAKAE
                     EVGSKPLARIVGYDQAAVEPRYIFAAPAKAIPRLLKRVGWSLSDVDLLEVNEAFAAQV
                     LANGQELSDQGWDWSKVNVNGGAIALGHPLGATGARILTTLVHALAQRDLHRGIAALC
                     LGGGEAVAVAVER"
BASE COUNT          539 a         1270 c         1270 g          581 t
ORIGIN      
        1 ggcgtaagtg cgtcacgggg tgtcggattc cgcgccgagc gcgggcaggt gctttgcact
       61 acaatccgcg agaatccgcg agcgcgcgac ctgatcgggc gcgtgagagg ggacgcattg
      121 gccaccggca cgaccgagca ccaccacacg gcgcgcaatc tcaagggttg gctgctgcag
      181 ggctacggcc acgagatggc ggggccgtac gagcgggaag agcaccacga gacgcgcccc
      241 tggtggcagg tgatgtgtct gaccggcctc gactacttct cgacgttggg ctaccaaccg
      301 gggatcgcgg cgctggcggc cggcgcgctg gctccgatcg cgacgctcgt gctggtgctc
      361 ctgaccctct tcggggcgct gccggtctac cgtcacgtgg ccgaggagag cccgcacggg
      421 cagggctcga tcgccatgct ggagcggctg atgggctggt ggaaggggaa gttcttcgtc
      481 ctggcgctgc tcggtttcgc cgccaccgac ttcatgatca ccatgacgct ctcctcggcg
      541 gacgccaccg cgcacatcgt cgagaacccg ttcaccccgt ccgtgctgca cggccacacg
      601 atcccgatca cgctggcgct gttgctgatc ctcgggttgg tctttctgaa gggcttcgcc
      661 gaggcgatca gcatcgcggt ggtgttggtc ttcagttacc tggcgctcaa tctggtggtg
      721 atcggcgact gcctctggca cgtcctccag cacccggcgc tggtcaccgc ctggcgccac
      781 gcgctggtgg tcgagcaccc cagcctgctg ctgatggcgg tctacgccgt gttggtcttc
      841 ccgcagctgg cgctgggcct ctcgggcttc gagaccgggg tggcggtgat gccgctggtg
      901 cgcggcgacg gccccgaggg cacgtctatc gacgggcgga tcaagaacac caagaagctg
      961 ctgaacaccg ccgccctgct gatgagcttc tacctgatca ccagctcctt cgtcaccatc
     1021 gtgctgatcc ccagcgcggc cttccaggtg ggtggggccg ccaacggccg cgcgctcgcc
     1081 tttctcgcgc atcagtatct cggcagcgcc ttcggcaccg tctacgacgt gagcaccatc
     1141 ttcatcctct ggttcgccgg cgcctcggcc atggccgggc tgctcaacct ggtgccgcgc
     1201 tacctgccgc gctacggcat ggcgccggaa tggacgcgcg ccgtacgccc gctggtggtc
     1261 ctcttcaccg tgatcgcctt cgccatcacc ctgatcttcg acgccagcgt cgacaagcag
     1321 ggcgcggcct acgccaccgg cgtactggtg ctgatgacct cgggcgccat cgcggtgacg
     1381 ctctcggtgg ccgacctgcg gcgccgctgg ctggtggtgg cgttcggctt catcgccgcg
     1441 gtcttcatct ataccaccat cgccaacgtc ttcgagcggc cggacggcgt caagatcgcc
     1501 gccttcttca tcggcaccat cgtgatcatg tcgctggtct cgcgcgtctg gcgcgtgcgc
     1561 gagctgcgcg tgaccgagat cgaggtcgac ccggcggccg agcgcttcat agccgacgcc
     1621 gctggagtgg gcacgctgcg ggtgatcgcc cacgacccgg agcaccgctg gccgggcgag
     1681 tacgaagaca agctgcagca ggagtgcgag caccacaaca tcccctcggc cgacgtcttc
     1741 ttcctggagg tcaaggtgga ggacccgtcg gacttcgccg ggcggctgga cgtgcacggc
     1801 acgaccgtcg gaggcttccg catcttccgc attcagagca gcgccatccc caacgccatc
     1861 gccgccttcc tgctgtatct acgcgaccgc accggcaaga tccccaacgc ctacttcgac
     1921 tggtcggagg ccgggccgct ggtgcagctc tcgcgctacg tcttcttcgg tgagggcgac
     1981 atcgcgccgg tcacccacga agtcctgcgc caggccgagc ccctccccga ccgccgcccg
     2041 atgatccacg tggggtagcc ctcatcccag gattaggagc aagggagaca gggggcgtga
     2101 gagaggcgac gcatacgatc ggcgtcgtaa taggcctcag ccttcccccc tgacattctg
     2161 agcgccgtcg aaggatccgt ccctacacac gccccgtgtt cctcggcagc cggcatccgt
     2221 tgcccgtggc ccggcgcgtg cttgcttgca ctggcgctcg gtgcaacact gagttgacga
     2281 tagcggcagc gatgcggagg aacgaccatg agcggcaacg gcaaagccaa ggcgaatcaa
     2341 cctgtcatcc tgagcgcggc ccgcacgccg atcggacgct tccagggcgc gctggccaac
     2401 gtgccggccg cggaactcgg cgcgaccgcc atccgcgcgg ccgtcgagcg cgccggcatc
     2461 gaggggcacc tgggcgagat cgacgaggtg ctgatgggca acgtcgtctc ggccgggctg
     2521 gggcagaaca tcgcgcgcca gtgcgcgctc aaggccggca tccccagcac ggtcggcgcg
     2581 atcacggtca acaaggtctg cggctccggc ctgaaggcgg tcatgctcag cgcccaggcg
     2641 atccgggccg gcgacggcga cctcttcgtg gccggcgggg tcgagagcat gagccgcgcg
     2701 ccctacctgg tccacggccg gctgggcgag ctgcgctacg gcaacgccga gatgaaggac
     2761 tcactggtct ccgacggcct ctggtgcgcg atcgagaact ggggcatggg caacgccgcc
     2821 gagtttatcg ccgacgagta cggggtcagc cgcgaggcga tggaccagta cgcgctcgcc
     2881 agccatcaga aggccgtggc ggccatggac gagggccgct tcaaggatga gatcgcgccg
     2941 gtgacggtgc cgggccggcg cgggcaggag acggtggtcg agcaggacga ggcgccgcgc
     3001 cgcgacacct cgctggaggc gctcagcaag ctgcgcccgg ccttcgtagc cgaggggcgc
     3061 gtgacggccg gcaacgcgcc gggtctcaac gacgccgccg ccgcgctggt gatcgccagc
     3121 gccgccaagg ccgaggaggt gggctccaag ccgctggcgc gcatcgtcgg ctacgaccag
     3181 gccgccgtcg agccgcgcta catcttcgcc gcgccggcca aggcgatccc gcgcctcttg
     3241 aagcgcgtcg gctggtcgct cagcgacgtc gacctgctgg aggtcaacga ggccttcgcc
     3301 gcccaggtgc tggccaatgg gcaggagcta tcggatcagg gctgggactg gagcaaggtc
     3361 aacgtcaacg gcggcgccat cgcgctcggc cacccgctgg gcgccactgg ggcgcgcatc
     3421 ctgaccacgc tggtccacgc gctggcgcag cgcgatctgc accgcggcat cgccgcgctg
     3481 tgtctgggcg gcggcgaggc cgtggccgtg gcggtcgagc gctaggcggc gcgcatggcc
     3541 gccgatgtcg agctgctgat cgtcggcgcc gggccgttcg gcctggcgct ggcggcccac
     3601 gccgctgacc tcgggctcga ctatctgatc gtgggcgagc cgatgggctt ctggaagcgc
//
LOCUS       DASUBI010000814         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_388820, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000814 DASUBI010000000
VERSION     DASUBI010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388820"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..779
                     /locus_tag="VFU72_16020"
     CDS             324..779
                     /locus_tag="VFU72_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425049.1"
                     /translation="MLLDTRLWILIGLCVAMLFTVEAIEDYVEGTWPQMRRPMSGAGV
                     VGSARALWVAVALFVLPGLVLTILNLGILVWQNLPYANLQLLGTIFVVVGWLVFLSTT
                     IDLGGIGTYFEDVGFVAPLSLVLILAIGDLLLLITLIDIFPHNLHTLLP"
     gene            complement(855..2228)
                     /gene="dnaA"
                     /locus_tag="VFU72_16025"
     CDS             complement(855..2228)
                     /gene="dnaA"
                     /locus_tag="VFU72_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5425050.1"
                     /translation="MQATQIWQAALSELEAQLSRANYETWLRNTQIITWEDDCATIGA
                     PNSFAVDQLRSKFAVQIQSALSTIVGRTVAVQFDLLNSQQRANRPARQRERGGTATAE
                     PAPQQLELSEIPEHGLNPRYTFEKFIVGPNNRLAHAAALAVADRPAYKFNPLFIYGGV
                     GLGKTHLLHAIGHRALSRNPQLQIRYVSSETFTNEMVNAIRQNRNEEFRNRYRTIDIL
                     MVDDIQFIAGKESTQEEFFHTFNALHQSGKQVVLSSDRPPKAIQTLSDRLRSRFEGGL
                     LADVQTPDLETREAILAEKGLEMNVVVPTDVLEYVARKVQSNIRELEGALNKIVVLGQ
                     LYSKPVTLELAIEALTEVARQERRRQITTELVIEAVTGYFKVSRRDLTGPGRRKDFVL
                     PRQVAMFLMREETETSLVEIGQALGGRDHSTVLHGIEKMNRQLENDPRLRGELLAIKE
                     QLYTARV"
BASE COUNT          374 a          797 c          876 g          491 t
ORIGIN      
        1 acgcgctcag ctgaggcagg ccggcacggc tcgtcgcctt cgacaacggt gagtgccggg
       61 tgaggacgga tccttcgacc gcgctcagga cgaccaaggg tggggctgag gcctaccgga
      121 tgcggcccat cctcctcgtc agcccgaacc aagcagcgtc atcctgagcg gagccgacgg
      181 atccgtcctc atgggcctcc ccctttccgc tgcggtaatg acgcctcact cgtggcacgc
      241 ggcaagcgga atggcccttg ccggcgacat cggcgtctgc tagcgtgcag gacagggaag
      301 gcgcgaatga gaggacggcg cgcgtgctgc tcgacacccg gctctggatc ctgatcgggc
      361 tctgcgtggc catgctcttc accgtcgagg cgatcgagga ctacgtcgag ggcacctggc
      421 cgcagatgcg ccggccgatg agcggcgcgg gcgtggtcgg cagcgcccgg gcgctctggg
      481 tcgcggtggc gctcttcgtg ctccccgggc tggtgctgac catcctcaac ctggggatcc
      541 tggtttggca gaatctcccc tacgccaacc tgcaattgct gggcacgatc ttcgtcgtcg
      601 tcggctggct cgtctttctg tcgacgacga tcgatcttgg cggcatcggc acttacttcg
      661 aggatgtcgg cttcgtggcg ccgctgtcgc tggtgctgat tctggcgatc ggcgatctcc
      721 tgctgctgat taccctcatc gacatctttc cccacaacct gcacacgtta ctaccctgag
      781 cgaacgtagt accggtcgcg cctggccgct gggcaggcgc gggccggttt ttgccgcggg
      841 acagcggagt ctccctacac gcgggcggtg tagagctgct ccttgatcgc caggagctcg
      901 ccgcgcaggc gggggtcgtt ctcgagctgg cggttcatct tctcgatgcc gtgcaggacc
      961 gtgctgtggt cgcggccgcc gagcgcctgg ccgatctcga ccagggacgt ctcggtctcc
     1021 tcgcgcatga ggaacatggc cacctggcgc ggcaggacga agtccttgcg gcggccgggt
     1081 ccggtcaggt cgcgccggga gaccttgaag tagccggtca cggcctcgat caccagctcc
     1141 gtagtgatct ggcgccgccg ctcctggcgc gccacctcgg tcagcgcctc gatcgccagc
     1201 tccagcgtga ccggtttgct gtagagctgg cccagcacca cgatcttgtt gagcgcgccc
     1261 tccagctcgc ggatattgct ctgcaccttg cgcgcgacgt actccaacac gtcggtcgga
     1321 acgaccacgt tcatctccag gcccttctcg gccaggatcg cctcgcgcgt ctccagatcg
     1381 ggcgtctgca cgtcggccag caagccgcct tcgaaacgcg agcgcagccg gtcggagagg
     1441 gtttggatcg ccttgggcgg gcggtcgctg ctgagcacga cctgcttgcc cgattgatgc
     1501 agcgcgttga aggtgtggaa gaactcctcc tgggtgctct ccttgccggc gatgaactgg
     1561 atgtcgtcga ccatcaggat gtcgatggtg cggtagcggt tgcggaactc ctcgttgcgg
     1621 ttctgccgga tagcgttgac catctcattg gtgaaagtct ccgaggagac gtagcggatc
     1681 tggagctggg ggttacgcga gagcgcccgg tggccgatgg cgtgcagcag atgcgtcttg
     1741 cccagcccga cccccccata gatgaagagg gggttgaact tgtaggccgg ccgatcagcc
     1801 acggccagcg ccgcggcgtg ggcgaggcgg ttgttggggc cgacgatgaa cttctcgaag
     1861 gtgtaacggg ggttcagccc gtgctcgggg atctcgctca actcgagttg ctgcggggcc
     1921 ggctcggcgg ttgccgtgcc gccgcgctct cgctggcggg cggggcggtt ggcgcgctgc
     1981 tggctgttga gcaggtcgaa ctgcaccgcg acggtgcggc cgacgatcgt cgagagagcc
     2041 gactggatct ggacggcgaa cttgctgcgc agttgatcga ccgcgaagga gttgggcgcg
     2101 ccgatggtgg cgcagtcgtc ctcccaggtg atgatctggg tgttgcgcag ccaggtctcg
     2161 tagtttgccc gtgagagttg cgcctccagc tcgctgaggg ccgcctgcca gatctgcgtg
     2221 gcctgcatcg cgctctcccc tgccagggtg tacgtacacc atgcggctca atcctagcat
     2281 ggcgtctgag ccggcgcaag caagcatagc gcgtcggagc agctggaaaa gacacaggtc
     2341 gggccatttg gcccgacctg tgcgggaagg ggaggggatg gagcatcgct tgctccgtag
     2401 gggatgcgag agcgcgcttg gggggagcgt ctctctactc tcatatacgt caaccctggc
     2461 agtttggttc catgtcctcg ccgcgttctc ggcgccaaac gcgttgattg catcgaagca
     2521 ttccggtaca ctgatggg
//
LOCUS       DASUBI010000815         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_259678, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000815 DASUBI010000000
VERSION     DASUBI010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259678"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1696
                     /gene="glpD"
                     /locus_tag="VFU72_16030"
     CDS             38..1696
                     /gene="glpD"
                     /locus_tag="VFU72_16030"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEU5425051.1"
                     /translation="MVAAERMTPIPDDIERQPFDVIIIGAGINGAGIARDAALRGLKV
                     LLLDKGDIASGTTARSTRLIHGGLRYLEYYEVPLVRESLREREILLRIAPHLVDPLQF
                     LIPIYAGGKRGPGLIRLGMIAYDILSYDKSMANHRMLSREQTLALEPGLNPEGLRGAA
                     LYYDAQITFPERLAVENALSARDHGAVVLTYCQVERPLTDGQGTVTGVTFTDLIGEGG
                     REYTVRAPVTVNVAGPWVDKVLGDLDPGRYIGGTKGSHIVVDAFPGAPKEALYVEASA
                     DRRPYFIVPWNGRYMIGTTDLRYTGNLDHVVPDEEELTYLIDETNHVIPSAHLTRGDV
                     LYAYAGVRPLPYKEEGTTGSITRKHIIHPHDAPLNRMISIVGGKITTYRGLAEETTDL
                     IFELLGRPVPPALTGKIPLPGGNTDDWAAFAADFTATSGLESAVAQRLLRIYGVMARQ
                     ILRIAAGDPSLRQVFSPDSGAIAAEVPYAVRWELAETLEDILMRRGMVGFDDSTGLDA
                     LDNAAAIAQRTLGWDPARAQQETAAYRQYLQQFHPRALAKQAPL"
     gene            2019..>2411
                     /locus_tag="VFU72_16035"
     CDS             2019..>2411
                     /locus_tag="VFU72_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425052.1"
                     /translation="MGGDQLVVKRIVVLANSYKHDGRCVAGREVAVAEDGRVTFGGWL
                     RPVNDTEDGELLPEQCRLQDGSLPRPLDIIDIPCSGPQGDPTQPENWLLAGGTNWRRA
                     GRLSPDHLPELAERPAQLWPDDSSYTDRV"
BASE COUNT          407 a          866 c          794 g          344 t
ORIGIN      
        1 gcggcgcggg cggccctcgg cagagagtga ggcaggtatg gtcgcggcag agcgaatgac
       61 cccgattccg gacgatatcg agcggcagcc gttcgacgtc atcatcatcg gcgccggcat
      121 caacggcgcc ggcatcgcgc gcgacgccgc gctgcggggg ctgaaggtgc tgctgctcga
      181 caagggcgac atcgccagcg gcacgaccgc ccgctcaacc cgcctgatcc acggcgggct
      241 gcgctacctg gagtactacg aggtaccgct ggtgcgcgag tcgctgcgcg agcgcgagat
      301 cctgctgcgt atcgcgcctc acctggtcga tcctttgcag ttcctgatcc cgatctacgc
      361 cgggggcaag cgcggccccg gcctgatccg gctcggcatg atcgcctacg acatcctctc
      421 ctacgacaag agcatggcca accaccgcat gctcagccgc gagcagacgt tggcgctgga
      481 gcccggcctc aaccccgagg ggctgcgcgg cgccgcgctc tactacgacg cccagatcac
      541 cttccccgag cggctcgcgg tggagaacgc gctctcggcg cgcgaccacg gcgcggtggt
      601 gctcacctac tgccaggtcg agcgcccgct gaccgatggt caggggaccg tgaccggcgt
      661 caccttcacc gatctcatcg gtgagggtgg gcgcgagtac accgtgcgcg cgccggtcac
      721 ggtcaacgtc gccggcccct gggtcgacaa ggtgctgggc gatctcgatc ctggtcgcta
      781 catcggcggg accaagggga gccacatcgt ggtcgacgcc ttccccggcg cgcccaagga
      841 ggcgctctac gtcgaggcca gcgccgaccg gcggccgtat ttcatcgtcc cctggaacgg
      901 gcgctacatg atcggcacca ccgacctgcg ctacaccggc aacctggacc acgtcgtgcc
      961 ggacgaggag gagctcacct acctgatcga cgagaccaac cacgtcatcc cctcggccca
     1021 cctgacgcgc ggcgacgttc tctatgccta cgccggggta cgcccgctcc cctacaagga
     1081 agaaggaacg acggggagca tcacccgcaa gcacatcatt cacccgcacg acgcacccct
     1141 gaaccgcatg atctcgatcg tcggcggcaa gatcaccacc taccgcggcc tggccgaaga
     1201 gacgaccgac ctgatcttcg agctgcttgg ccgcccggtg ccgcccgcgc tgaccggcaa
     1261 gatcccgctc cccggcggga ataccgacga ctgggccgcg ttcgccgccg acttcacggc
     1321 gacgtccggg ctggagagcg ccgttgccca gcggctgctg cgcatctacg gcgtgatggc
     1381 gcgccagatc ctgcgcatcg ccgcaggcga ccccagcctg cggcaggtct tcagccccga
     1441 cagcggcgcc atcgcggccg aggtgcccta cgccgtgcgc tgggagctgg ccgagacgct
     1501 ggaggacatc ctgatgcgcc gcggtatggt cgggtttgac gactcgacgg ggctggacgc
     1561 gctcgacaac gccgccgcca tcgcccagcg cacgctcggc tgggacccgg cgcgcgccca
     1621 gcaggagaca gccgcctacc gccagtatct ccagcagttc cacccccgcg ccctggccaa
     1681 gcaggcaccc ctctgacatc ctgagccctg aatctgcccg gtcatcctga gccgagcttg
     1741 tcgaagggat ctctgatcgg agtcgagcac ccggattgtt gtcagacagt agagaccctc
     1801 cggctgcggc ctcaggatga ccggatggac gacgggccac cgtcaatcgt ggcgagcatg
     1861 gacagctccc ttgcgcaccg acccttgacc aggtccacca accggctaca ctgaagcgga
     1921 ttcgtcgatc aatcgcagat gtgccgttgg cgacgtaatg gagcgcgcgg cgccctatgc
     1981 gcgccggggc gcgatcggcc gcggatcagg gagcggagat gggtggcgat cagctggtcg
     2041 tcaagcgcat cgtcgtgctg gcgaactcgt acaagcacga cggccgctgc gtggccggcc
     2101 gcgaggtggc agtcgcggag gacgggcggg tgaccttcgg cggctggctg cggccggtca
     2161 acgacaccga ggatggcgag cttcttccgg aacagtgccg gctacaggat ggcagtctcc
     2221 cacgcccact cgacatcatc gacattccct gcagcgggcc acagggagac ccgacgcagc
     2281 cggagaactg gctgctcgcc ggcggcacga attggcgacg ggcgggccgg ctcagccccg
     2341 atcacctgcc cgagctggcc gagcgcccgg cacaactctg gccggatgat agctcctaca
     2401 ccgaccgcgt c
//
LOCUS       DASUBI010000816         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_566001, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000816 DASUBI010000000
VERSION     DASUBI010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566001"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="VFU72_16040"
     CDS             complement(<1..1693)
                     /locus_tag="VFU72_16040"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5425053.1"
                     /translation="MAHRRLVIGGWIGALVVAMGAAHAAKPDYVNDLALNGTDSQRVI
                     ATLHGAFRSQAGDLDEIVLSTPKRDVASVQVQSQVAAMLARVRRLPHVVAVGGPYARN
                     SQGALSPDRHTAFATVRFDQPAERLDPATAQRVIDTAQLARTSDLEVQLGGRGIQQAN
                     RPSTGAATAIGLVAALTILLFTFGSLLAAALPILTALLGLGTALGLVGLQSHVVHTPD
                     FAPQLAALLGLGVGIDYALFVVTRFREALRDDVSVQDAVVTAMDTAGRAVLFAGTTVV
                     IALAGMFVLRVGILSASAFASALSVLLTMSAALTALPVALASAGRRLAPRKEKGAGLW
                     PRWADAVVKRPWAALAAGLALMLVLTIPAFTLRLGQSDAGNDPAHTTTRRAYDQLARA
                     FGAGFNGPLEVVARQSPGGADSQAAVAAALRATPGIRTVSDPVRSADGRTLVYQAFAS
                     TAPESKATADLVQRLRDTTLPAVERSTGAHILVGGATATGIDFSAVVGRRLGWFIAAV
                     IVLAALLLTVVFRSAAVPLQAAIMNLLSVGAAYGVIVAVFQMGWGANLLGVQAKGP"
     gene            complement(1732..>2006)
                     /locus_tag="VFU72_16045"
     CDS             complement(1732..>2006)
                     /locus_tag="VFU72_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5425054.1"
                     /translation="KPVTFLSYGGAAGGVRAVEQLRQVAIELDMVPLRRHVAISGIFR
                     RLDEDRRLIDPPLVEAQLMFDDLVWWATTLAQARNTAGAEDAASAA"
BASE COUNT          327 a          733 c          693 g          253 t
ORIGIN      
        1 tcgggccttt cgcctgtacg ccgagcaggt tcgcgcccca gcccatctgg aagacggcca
       61 caatcacgcc gtacgccgcc cccaccgaga gcaggttcat gatcgcggcc tgcagcggta
      121 ccgccgccga ccggaagacc acggtcagca acaacgccgc gaggacgatc accgccgcga
      181 tgaaccagcc cagcctgcga ccgacgacag cgctgaagtc gatcccggtc gccgtcgcgc
      241 cgcccaccag gatgtgagcg ccagtcgacc gctcgaccgc cggcagcgtc gtgtcccgca
      301 gccgctgcac gaggtcggcg gtggccttgc tctctggtgc cgtggaagcg aacgcctggt
      361 agacgagcgt ccggccatcg gcgctccgca ccgggtcgct caccgtgcgg atgccgggcg
      421 tagcccggag cgccgctgcg accgccgcct gagaatcagc gccgcccggc gattggcgcg
      481 ccacgacctc gagcggaccg ttgaagcctg cgccgaatgc ccgcgccagc tggtcatagg
      541 cccggcgcgt cgtcgtgtgc gccggatcat taccggcgtc gctctgcccg agccgcaggg
      601 tgaaggcggg gatggtcaga accagcatca aggccaagcc cgcggcaagg gccgcccacg
      661 gccgcttgac caccgcgtct gcccaccgtg gccacagacc cgctcctttc tccttgcgcg
      721 gagcgagccg gcgcccggcg gacgcaagcg ccaccgggag cgccgtcagc gcggcgctca
      781 tggtcagcag gacgctgagc gcgctcgcaa aagccgacgc cgagaggatg cccacccgca
      841 gcacgaacat gcccgccagc gcgatcacga ccgtcgtacc cgcgaagagg acggctcggc
      901 ccgcagtgtc catggcggtg acgacggcgt cctggaccga tacgtcgtcc cgcagcgcct
      961 cgcgaaaccg ggtcaccacg aacagggcat agtcgatccc gaccccgagg ccgagcaacg
     1021 cggccagctg gggcgcgaag tcgggcgtgt ggacgacgtg tgactgcagt cccaccagcc
     1081 ccagggcggt tcccagcccc agcagagcgg tcaggatcgg cagggccgcc gcgagcaacg
     1141 atccgaatgt gaacagcagg atcgtcaggg cggccaccag gccgattgcg gtagccgctc
     1201 cggtcgaggg gcggttggcc tgttggatgc cgcggccccc cagctgcacc tccagatcag
     1261 acgtccgcgc cagctgggcg gtatcgatca ctcgttgcgc ggtggcgggg tcaaggcgct
     1321 ccgccggctg gtcaaagcga accgtggcaa aggcggtgtg gcgatctggc gagagcgccc
     1381 cttggctgtt gcgcgcgtag ggaccgccca cagccacgac gtgcggaagc cggcgcaccc
     1441 gcgcgagcat cgccgcgacc tggctttgga cctggacact tgccacatcg cgcttgggcg
     1501 tgctgagcac gatctcgtcg agatcgcctg cctgcgagcg aaaggcaccg tgcagcgtgg
     1561 cgatcacccg ttgcgaatcg gtcccgttca gcgcgagatc gttgacgtag tcgggcttgg
     1621 ccgcgtgcgc tgcgcccatc gccaccacca gcgcgccgat ccagcctccg atgaccagcc
     1681 ggcgatgagc catggcccag gccgcgagcc gcccgatcca accagggtgc ctcacgcggc
     1741 ggaggcggcg tcctcggcgc ccgccgtgtt gcgcgcctgc gcgagcgtgg tcgcccacca
     1801 gacgaggtcg tcgaacatca gctgggcctc gacgagcggc ggatcgatga gccggcggtc
     1861 ctcgtccagg cgccggaaga tccccgagat cgcaacgtgc cggcggagcg ggaccatgtc
     1921 cagctcgatc gcgacctggc gaagctgctc gaccgcgcgg accccgcccg cggcgccgcc
     1981 gtaggacagg aaggtcacgg gcttgc
//
LOCUS       DASUBI010000817         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_836125, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000817 DASUBI010000000
VERSION     DASUBI010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836125"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFU72_16050"
     CDS             complement(<1..229)
                     /locus_tag="VFU72_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425055.1"
                     /translation="MDLHLIPNAEATEEERAAVDALLGSPETAWEGAVERSPFEGHAG
                     RGGHALRAQRHLLLPALQALQARAGYISAGGL"
     gene            complement(230..2131)
                     /locus_tag="VFU72_16055"
     CDS             complement(230..2131)
                     /locus_tag="VFU72_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1919..1921),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5425056.1"
                     /translation="MREGGVLRPASWDEALERAAAGFRAAVERHGPDTFGMFSCSKAT
                     NEVNYIAQKFARQVIGTNNVDSCNRTUHAPSVVGLTTVLGAGGGTSSYQEAEHTDLII
                     LWGSNARETHPIYFHHVLRGLRNGAKLYVVDPRRTTSAQWADGWLGLDVGSDIALANA
                     IGREIIAAGLTNPEFIERATSGFEAYREAVQPYTLAYAERLTGVPAELIRELAHAYAR
                     ADRAQLCWTLGITEHHNATDNVFSLINLALLTGHVGRYASGLVPLRGQNNVQGGGDMG
                     AIPNKLPGGADVEIDADRAPFERVYGVRIPPKRGRDLTQMFEAIEHGQMSTLYIVGEN
                     PAQSEADMGRTVRLLAGLDHVVVQDIFLTKTGELADVVLPASASWCEAEGTVTSSDRR
                     VQRVRKALEPPEGARDDIAIIADIARRLGFDWGQPSAEQVWDELRSLSSWHRGMSYAR
                     LEGPISLHWPCPAEDHPGSPYLHERLWHNPIEGPRAAFHPAEFEAPVEALSEEFPIRL
                     TTGRRLDSFNTGVQTGGYTSPLRRGETLDIAPEEGRRLGIADGEVVRVVSRRGAVEAP
                     VRFDPTLRPGLAFMTLHFQDAVATNILTIDATDPRSGTAEFKAAAIRVEKLSGDRAPV
                     LAGAVAREK"
BASE COUNT          297 a          740 c          779 g          317 t
ORIGIN      
        1 tgaggccacc ggcgctgatg tagccggcac gcgcctgcag cgcctggagc gcgggcagca
       61 gcaggtggcg ctgggcgcgc agcgcgtggc cgccgcgccc ggcgtgcccc tcgaagggcg
      121 agcgctcgac ggcgccctcc caggcggtct ccggcgaacc gagcagcgcg tcgaccgcgg
      181 cgcgctcctc ctccgtcgcc tccgcgttgg ggatcaaatg caggtccact tacttctccc
      241 gggcgacggc tccggccagc accggtgcgc ggtcaccgct cagcttttcg acccggatgg
      301 ccgcggcctt gaactcggcc gtgcccgagc gcgggtcggt cgcgtcgatc gtcaagatgt
      361 tggtcgccac cgcatcctgg aagtggagcg tcatgaaggc caggccgggc cgcagcgtgg
      421 gatcgaagcg cacgggcgcc tcgaccgcgc cgcgccgcga gacgacgcgc accacctcac
      481 cgtccgcgat ccccaggcgc cgcccctcct ccggcgcgat gtcgagtgtc tcgccgcgcc
      541 gcaggggcga ggtatagccg ccggtctgga caccggtgtt gaacgagtcg agccggcgcc
      601 cggtcgtcag gcggatcggg aactcctcgg agagcgcctc gaccggcgct tcgaactccg
      661 ccgggtggaa ggccgcgcgc gggccctcga tcgggttgtg ccagagccgc tcgtgcaggt
      721 aggggctgcc cggatggtct tcggccgggc agggccagtg gagcgagatg ggcccctcca
      781 gccgggcgta gctcatgccg cggtgccagg aggagagcga gcgcaattcg tcccagacct
      841 gctcggcgct gggttggccc cagtcgaagc cgagccggcg cgcgatatcg gcgatgatcg
      901 cgatgtcgtc gcgggcgcct tcgggcggct ccagcgcctt gcgcacgcgc tgcacgcgcc
      961 ggtcggagct ggtgaccgtg ccctccgcct cgcaccacga cgccgaggcg ggcagcacaa
     1021 catcggctag ctcgccggtc ttggtcagga agatgtcctg caccaccacg tggtcgagcc
     1081 cggccagcag gcgcacggtg cggcccatgt cggcctccga ctgcgccggg ttctcgccga
     1141 cgatgtagag ggtgctcatc tggccgtgct cgatggcctc gaacatctgc gtcaggtcgc
     1201 gcccgcgctt gggcgggatg cgcacgccgt agacgcgctc gaagggagcg cgatcggcgt
     1261 cgatctcgac gtctgcgccg ccgggcagct tgttggggat agcgcccata tcgccgccgc
     1321 cctgcacgtt gttctgcccg cgcaggggca ccaggccgga agcgtagcgg ccgacgtggc
     1381 cggtcaagag cgccaggttg atcagcgaga agacattgtc ggtggcgttg tggtgctcgg
     1441 tgatgccgag cgtccagcag agttgcgccc ggtcggcgcg ggcataggcg tgcgccagct
     1501 cgcggatcag ctccgccggc acgccagtca ggcgctcggc gtacgccagc gtatagggct
     1561 ggaccgcctc ccggtacgcc tcgaagccgc tggtggcacg ctcgatgaac tcgggattgg
     1621 tcagaccggc cgcgatgatc tcgcgcccga tggcgttggc cagcgcgata tccgagccga
     1681 catcgagccc gagccagccg tcggcccact gggccgaggt ggtacggcgg ggatcgacca
     1741 cgtagagctt cgcgccgttg cgcaggccgc gcaggacgtg gtggaagtag atcgggtgcg
     1801 tctcgcgcgc gttcgagccc cacaggataa tgaggtcggt gtgctccgcc tcctgatacg
     1861 agctggtgcc gcccccggca cccaggaccg tcgtcagacc gacgacgctg ggcgcgtgtc
     1921 aggtacggtt gcaggagtcg acgttgttgg tcccgatcac ctgccgggcg aacttctggg
     1981 cgatgtagtt gacctcgttg gtcgctttcg agcaggagaa catgccaaag gtgtcgggcc
     2041 catggcgctc gaccgcggcg cggaagccgg cggcggcacg ctccagcgcc tcatcccagc
     2101 tcgcgggccg cagcacgccg ccctcgcgca cca
//
LOCUS       DASUBI010000818         7352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_906793, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000818 DASUBI010000000
VERSION     DASUBI010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906793"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFU72_16060"
     CDS             complement(<1..197)
                     /locus_tag="VFU72_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425057.1"
                     /translation="MSMTWHEQEPPQPAGSDAPPGAVELGQDVFALDGQRVGSVANIE
                     PGYLVITRGPLNPPERAIPRGL"
     gene            376..837
                     /locus_tag="VFU72_16065"
     CDS             376..837
                     /locus_tag="VFU72_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425058.1"
                     /translation="MGFLDQLLGNDQPQQKQDYQDFIKRYEQGTPGDGYSDQEVLNRY
                     QQVAPELNQQQYLSAAEQAFQRMSPQERAQFAQYLEQQARQNNLTVPQQPAQHYAQNP
                     GALAQLTSQVHREQPGLLDQLLGGNGGGLNNPIAKAALAGIAAMAAKRFLG"
     gene            complement(890..2155)
                     /locus_tag="VFU72_16070"
     CDS             complement(890..2155)
                     /locus_tag="VFU72_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5425059.1"
                     /translation="MGFLRLLRRRHLAILWLSQVLSAIGDNLYAIAVVWLAVQVAGSG
                     AGLVVAAQSISALAFGLLGGVFADRWDRRRTMMAVDLARGAAVAVLPVLAWLDMLQLW
                     HMAAVAVVIGGLGALFDPALLASLPALTGDAATLQAANGLMDITRRLARALGPGLVGV
                     LVALLPVAQFFTLDAVSFAISAGAVYALGRGYAWRPRAAAGARGAAGVLHEIGGALRL
                     VRAHRPLTWALAGLGVSNIAWSTAFIVGVPLLAERELAGSIGAFGLIVGAYGAANVVG
                     NLIIGSLAIKRRALVLFSGRVVLGGGFLILAVAHNLPLAMLGSGLAALGGPIGDLMML
                     TMIQTDFASDQIGKVFALRMTISSAGVSLGLLLAVPLFAHLSIALVIAASRASPAPPM
                     PRFNRRRRPPTRRCPPNTLGAGRGTQCGR"
     gene            2298..3671
                     /locus_tag="VFU72_16075"
     CDS             2298..3671
                     /locus_tag="VFU72_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425060.1"
                     /translation="MSRSLSLSLARVPVALRRLPPGRAGARTLLHAAPPCLAAATLLA
                     FGYQRYAWGTAHAQEYQQQAYAHLRYSDIIWLYLRDHLATHPVPYLDYRLEYPVLLGF
                     WMYLLSFTGSLSAFFAANYALLAAGALLTVLLLGLLAGADRWRLALAPALVLYTGLNW
                     DMAAIAATVVGLVLVQRRRDRPGTLALTIAVWLKLFPLVFLAAVLWERLRRRELRRSA
                     EIAAIFAGVSLLLNLPLALADWSRWSYFFTFNNDRAGNANLWVLWPHLAVDRGNLISL
                     GLLAAGGVVLAALAWRARLDVTLPLGAGLLVWWLAVNKIFSPQYALWVFLAMALVRAA
                     WPLWAAFAAADLAHFGLSWLLLYTASVAGNTGLVRWENHHLRDPVVLALGALMLVLVV
                     ECALRLWRGRGEDSCEQPGVKRVARWVRGRWRVDRRSDKAPGAADQQRLAAESKEAFR
                     ARAGAGG"
     gene            complement(3693..4853)
                     /locus_tag="VFU72_16080"
     CDS             complement(3693..4853)
                     /locus_tag="VFU72_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU5425061.1"
                     /translation="MSTSSSTEPTIHNGLEGVVVAATSLAKIEGNVGRLSYRGYSIAD
                     LAQHATYEEVLYLLWYGKLPNKQEYAQLKEKLTRARTLSPTVMKVIKDLPTTGAPIDA
                     LRAGVTAMGMEDPTEDDLEHNAVIEKSIHMAGAMPTMLAAYGRLREGKQPVDPDPSLD
                     DAANFLAMLRGEKPSELDSNAFNAYLVCLAEHSMNASTFSARVTISAISDVYSAIATA
                     LGTLKGVAHGGANMASMKMLFDIGSADNVEHYVDESLKSHRRLMGLGHRIYKARDPRV
                     DILMDYSEKVSQEQGNQTYHNIAKKLEELTGHHPFFLERKLFPNVEFYSAPLLYMLGL
                     QPDMMPGAFAISRIGGWTAHLLEQLEDNRLIRPSALYVGPDLQTYEPIDSRS"
     gene            5046..5267
                     /locus_tag="VFU72_16085"
     CDS             5046..5267
                     /locus_tag="VFU72_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5425062.1"
                     /translation="MINFEGVEYLTASEAAEMLGVKVPTLYAYASRGRVRSYRRGIRR
                     ERFYRRDEIEALLHIRPVAPPAPSEGPSG"
     gene            5319..5720
                     /locus_tag="VFU72_16090"
     CDS             5319..5720
                     /locus_tag="VFU72_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5425063.1"
                     /translation="MARILIVDDDPAIRGLLVELLSDEGYDTVQAADGLDAIALARNA
                     RPQVILMDLMLPVLDGAAATRILKSEPETAGIRIIAMSAGANLLLHADTLPADGVLSK
                     PFDLDALLAEVAIQLRKTGPLGLPELDERPD"
     gene            5704..6012
                     /locus_tag="VFU72_16095"
     CDS             5704..6012
                     /locus_tag="VFU72_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425064.1"
                     /translation="MNAPTNPPSPLYIWLGTDPLGTPPDTEIEDVPGVSDLQLLGEAL
                     AGGRFGTIIPAKIALSPHRSPSASGYRSVDVARLLRDLQIPHRRCYELLPRGPAESEG
                     "
     gene            complement(5970..6521)
                     /locus_tag="VFU72_16100"
     CDS             complement(5970..6521)
                     /locus_tag="VFU72_16100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5425065.1"
                     /translation="MIDFQPLGEDDLPLLHGWLQREHVARWWGETPEMQKVRDHYLPM
                     IRGEEQTDGYLIRLHGRPIGFIQTYRIADWQDTWPEVDEPGAAGMDLLIGEPDLIGHG
                     LGPCVIRTFVETIVFAPPDVRVCWADPDARNQRSVRAFEKAGFRAIRDVWAEDAQTTE
                     RLVRLGRDELQPSDSAGPRGSSS"
     gene            6689..6991
                     /locus_tag="VFU72_16105"
     CDS             6689..6991
                     /locus_tag="VFU72_16105"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEU5425066.1"
                     /translation="MRLTPKELDRLTVFTLAELARKRRARGLKLNHPESVALICDEIF
                     EEARAGAAYEAVVTHAGRVLSRDDVLPGVPEMIPVVQVDALFPDGTRLVTVRTPIR"
     gene            6988..>7352
                     /gene="ureB"
                     /locus_tag="VFU72_16110"
     CDS             6988..>7352
                     /gene="ureB"
                     /locus_tag="VFU72_16110"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEU5425067.1"
                     /translation="MSGELPPGAILAGEGTIEPNAGLERRALRVINTGEVPVQLTAYF
                     HVFEANPRLCFDRRRAFGMRPDVPIGGAVRIEPGATVELIVVPFTGRRQIYGFNGLVD
                     GPLNDADLDALLARLIDRG"
BASE COUNT         1119 a         2612 c         2493 g         1128 t
ORIGIN      
        1 aggccgcgcg ggatcgcgcg ctcgggtggg ttgagcggac cacgggtgat gaccaggtag
       61 ccaggctcga tgttggccac cgatccgact cgctggccgt ccagcgcgaa gacatcctga
      121 ccgagctcaa cggcgcccgg cggcgcgtcg ctcccagcag gttgcggcgg ttcttgctcg
      181 tgccaggtca tactcacacc atcacctttc gctcgcttcg tccacgccct cggcggctct
      241 gttcgccggc cgagcggcgc gatcagagcc ctaacgcaag tgggatgccc cgagcgagta
      301 gttcgtgaat ggcatagctc atgcacattg ctgggagccg cgacgcgcgg cgacacagac
      361 gtatgggagg tttacgtggg attcttggat cagttgctgg gcaacgacca gccccagcag
      421 aagcaggatt accaggattt catcaagcgc tacgagcagg gcacgcctgg cgacggctat
      481 tccgatcagg aggtgctcaa tcggtatcag caggtggcgc cggagctgaa tcagcagcaa
      541 tacctcagcg ctgccgagca ggctttccag cgcatgtcgc cccaggagcg agcccagttc
      601 gcccagtacc tggagcaaca ggcgcggcag aacaacctca ccgtgccgca gcagccggcg
      661 cagcactacg cgcagaatcc gggcgcgctg gcccaactga cgagccaggt gcaccgggag
      721 cagccgggct tgctcgatca gttgctcggc ggcaacggcg gcggcctgaa caacccgatc
      781 gcgaaggcgg ccctggccgg tatcgccgcg atggccgcca agcgcttcct gggctaggcg
      841 ctgtccgagg ggtcggacga cgggcccggg tggcgcggcc agccggtggc taacgaccgc
      901 attgggtgcc tctcccggca cccaacgtgt tcggcggaca gcgcctagtc ggcggcctgc
      961 ggcgccggtt gaacctcggc atcggcggcg cgggcgaagc gcgcgaggcc gcgatgacca
     1021 acgcgatcga cagatgggcg aagagcggca cggccagtag cagcccgagc gagacgcccg
     1081 cgctgctgat cgtcatgcgc agggcaaaga ctttgccgat ctgatcgctc gcgaaatcgg
     1141 tctggatcat ggtcagcatc atcaagtcgc caatcggtcc gccgagcgcg gcgaggcccg
     1201 agccgagcat cgccagcggc aggttgtgcg cgacggccag gatcaggaaa ccgccgccga
     1261 gcaccacgcg cccgctgaag aggaccagcg cccggcgctt gatcgccagg ctgccgatga
     1321 tcagattgcc caccacgttg gccgccccgt aggcaccgac gatcaggccg aaggccccga
     1381 tcgagccggc cagctcgcgc tcggccagca gcggcacgcc gacgatgaag gcggtactcc
     1441 aggcgatgtt ggagacgccc agcccggcca gcgcccaggt cagcgggcga tgcgcccgca
     1501 ccagccgcag cgccccgcca atctcgtgca gcacccccgc cgcgccgcgc gcgcctgccg
     1561 cggcgcgcgg ccgccaggcg tagccgcgtc ccagcgcgta gaccgcgccc gccgagatag
     1621 cgaagctgac ggcgtcgagc gtgaagaact gcgccaccgg cagcagcgcc accagcaccc
     1681 cgaccagccc cggcccgagc gcccgcgcca gccgccgggt aatgtccatc agaccgttgg
     1741 cggcctgcag cgtggcggca tcgcccgtca atgcgggcag gctggccagc agggcagggt
     1801 cgaacagggc gccgagcccg ccgatcacca ccgccaccgc ggccatgtgc catagctgca
     1861 gcatatcgag ccaggcgagc accggcagca cggccaccgc cgcgccccgc gccagatcga
     1921 cggccatcat ggtgcgccgg cggtcccagc ggtcggcgaa gacaccgccg aggaggccga
     1981 aagccagcgc ggagatcgac tgcgccgcca cgaccagccc ggcgccgctg ccggccacct
     2041 gcaccgccag ccagacgacc gcgatggcgt agaggttatc cccgatggcc gagagaacct
     2101 gactcagcca gaggatcgcc aggtgccggc ggcgcagcag gcgcaggaag cccacggcgg
     2161 gcgccgcctc tcagcccggc cgcgcggcgg tcggcagggg ccgcgcacta taatggtccg
     2221 cggccggcac gggcccgggt gggcgtactc gctgccgcgc gtcacgcccc agcgcttccc
     2281 gccgtccacg gaggagcatg agtcgaagtc tttccctctc actcgcacgg gtgccggttg
     2341 ccctgcgccg cctgccgcca ggccgcgctg gcgcgcgcac gctgctccac gcggcgccac
     2401 cctgcctggc cgcggctacg ctgctggcgt tcggctacca gcgctacgcc tggggcacgg
     2461 cgcacgcgca agagtaccag cagcaggcct acgcccacct gcgctactcc gacatcatct
     2521 ggctctacct gcgcgaccac ctcgccacgc acccggtgcc ctacctcgac taccggctgg
     2581 agtaccccgt cctgctcggc ttctggatgt acctactgag cttcaccggc agcctgagcg
     2641 cgttcttcgc cgccaactac gccctgctgg ccgccggcgc gctgctgacg gtgctgctgc
     2701 tggggctgct ggccggcgcc gaccgctggc gcctggcgct ggcacccgca ctggtcctct
     2761 acaccggcct caactgggac atggcggcca tcgccgcgac cgtggtcggg ctggtgctgg
     2821 tccagcggcg ccgcgaccgg ccgggcacgc tggcgctgac catcgccgtc tggctgaagc
     2881 tcttcccgct cgtctttctg gccgccgtgc tctgggaacg cctgcgccgg cgcgagttgc
     2941 gccgctcggc ggagatcgcc gcgatcttcg ccggcgtgag cctgctgctg aatctgccgc
     3001 tggcgctggc cgactggtcg cgctggagct atttcttcac cttcaacaac gaccgagccg
     3061 ggaacgccaa tctctgggtg ctctggccgc acctcgcggt cgaccgcggc aacctgatca
     3121 gcctggggct gctggcggcc ggcggggtgg tgctcgcggc gctcgcctgg cgggcgcgcc
     3181 tggacgtgac gctgccgctg ggggcggggc tgctggtctg gtggctggcg gtcaacaaga
     3241 tcttcagccc ccaatacgct ctctgggtct tcctggcgat ggcgctggtg cgcgccgcct
     3301 ggccgctctg ggccgcgttc gccgcggccg acctggccca cttcgggctc tcctggctgc
     3361 tgctctatac cgcctcggtc gccggcaaca ccggcctggt gcgctgggag aaccaccacc
     3421 tgcgcgatcc ggtggtgctg gcgctcggcg ccttgatgct ggtactcgtc gtcgagtgcg
     3481 cgctgcggct gtggcgcggg cggggcgagg actcgtgcga gcagccggga gtcaagcgag
     3541 tggcacgctg ggtccgcggt cgctggcggg tcgaccggcg aagcgacaaa gcgccaggcg
     3601 ctgctgatca gcagcgcctg gcggcagagt cgaaggaggc cttccgcgcg cgagcaggag
     3661 ccgggggatg aaggagcccc agcgggcgcc ccttacgacc gcgagtcgat cggctcgtag
     3721 gtctgcagat ccgggccgac gtagagcgcc gacgggcgaa tcagccggtt gtcctccaac
     3781 tgctccagca ggtgcgccgt ccagccgccg atgcgactga tggcgaaggc gcccggcatc
     3841 atatccggct gcaggccaag catgtacagc agcggcgccg agtagaactc gacgttcggg
     3901 aagagcttgc gctccaggaa gaaggggtgg tggccggtca gctcctccag cttcttggcg
     3961 atgttgtggt aggtctggtt gccctgctcc tgggacactt tctccgagta gtccatcagg
     4021 atgtcgaccc gcggatcgcg cgccttgtag atgcggtgac cgagccccat cagccggcgg
     4081 tggctcttga gcgactcatc gacgtagtgc tcgacgttgt cggccgagcc gatgtcgaag
     4141 agcatcttca tggaggccat gtttgcgccg ccgtgcgcca cccccttgag cgtgccgagc
     4201 gcggtcgcga tcgcgctgta gacatcggag atggccgaga tggtgacccg cgccgagaag
     4261 gtcgaggcgt tcatgctgtg ctcggccaga cagacgagat aggcgttgaa ggcgttgcta
     4321 tcgagctcgc tcggcttctc gccgcgcagc atggcgagga agttggccgc gtcgtccagc
     4381 gagggatcgg gatcgaccgg ctgcttgccc tcgcgcagcc ggccgtacgc ggccagcatg
     4441 gtgggcatcg ccccggccat atgaatggac ttctcgatga ccgcattgtg ctccaggtcg
     4501 tcctcggtcg ggtcctccat ccccatcgcc gtgaccccgg cccgcagcgc gtcgatcggc
     4561 gccccggtag tggggagatc tttgatcacc ttcatgacgg tgggggagag cgtgcgcgcc
     4621 ctagtcaact tctccttgag ctgggcgtat tcttgcttgt tcggcagttt gccgtaccag
     4681 aggaggtaga gcacttcctc gtaggtcgcg tgctgcgcca gatcggcgat gctgtagccg
     4741 cgataggaga ggcgccccac gttcccctcg atcttcgcca aactcgtcgc ggcgaccacg
     4801 accccttcga gcccgttgtg gatggtcggt tcggtagacg aacttgtaga catgtgcggt
     4861 tcctccctcg tcagccgggc tgcggcccgg accgctggcc ggtcgtacca ccgcctatcc
     4921 tagcacgcct cgccccacaa atatagacat ggcacgataa acgttgacga aggttgacag
     4981 atcgcgatgg tccactatac tgattccaga ccagcgctct gagagagtcg ggagcgagcg
     5041 gagccgtgat caacttcgag ggagtggagt acctgaccgc ctccgaagcc gctgagatgc
     5101 tgggggtcaa ggttcccacg ctctacgcct acgccagccg cgggcgcgtc cgcagctacc
     5161 ggcgcggcat ccgccgcgag cgcttctatc gccgcgacga gatcgaggcg ctcttgcaca
     5221 tccgtccggt cgcgccgccg gcacccagtg aaggtccttc gggctagtcg tacggtctgg
     5281 caccgaactt gctgccatgc gcccgtggtg gaggcatggt ggcacggatc ctcatcgtcg
     5341 acgacgatcc tgcgatcaga gggctgctgg tcgaattgct ctccgacgag ggctacgaca
     5401 cggtgcaggc cgccgatgga ctcgacgcca tcgcgctggc ccgcaacgcc cgcccgcagg
     5461 tgattttgat ggatctgatg ctccccgtcc tggacggggc cgctgccaca cgcattctga
     5521 aatccgagcc ggagaccgcc ggcatccgca tcatcgcgat gtcggccggc gcgaacctgc
     5581 tgctgcacgc cgacacgctc cccgccgacg gggtgctctc caagccgttc gacctcgacg
     5641 ccctgctggc cgaggtcgcc attcaactcc gcaagacggg gccgctgggg ttgccggagc
     5701 tcgatgaacg ccccgactaa cccgccgagc ccgctgtaca tctggctcgg caccgacccg
     5761 ctcggcacgc cgcccgacac cgagatcgag gatgtccccg gcgtctccga cctgcagctc
     5821 ctcggcgagg cgctggccgg cggccgcttt ggcacgatca tccccgccaa gatcgcgctc
     5881 tcgccccacc gcagccccag cgccagcggc taccgctcgg tcgacgtcgc ccgcctgctg
     5941 cgcgacttac agatcccgca ccgccgctgc tacgagctgc tgccgcgcgg gccggcggag
     6001 tccgagggct gaagctcgtc ccgcccgagc cgcaccaggc gctcggtcgt ctgcgcatcc
     6061 tcggcccaga catcgcggat cgcccgaaac cccgccttct cgaaggcgcg caccgagcgc
     6121 tggttgcgcg cgtcgggatc ggcccagcag acgcgcacgt ccggcggggc gaagacgatc
     6181 gtctcgacga aggtgcggat gacgcacggg ccgagtccgt ggccgatcaa gtccggctcg
     6241 ccgatcaaga ggtccatccc ggccgccccc ggctcatcga cctccggcca ggtgtcctgc
     6301 caatcggcga tgcgataggt ttggatgaag ccgatcggcc gcccgtgaag cctgatcagg
     6361 tagccgtcgg tctgctcctc gccgcggatc atcggcaggt agtgatcgcg caccttctgc
     6421 atttccggag tctcgcccca ccagcgcgcc acgtgctcgc gctggagcca gccgtgcagc
     6481 agcggcaggt cgtcttcgcc gaggggttgg aagtcgatca tgcggggatc ctttcatggg
     6541 aaggttgccg ctttccgcgg agagtcgcgg tcgtcggccg cgcgcaccgg ctccgttggc
     6601 ggcgtccacc ttcgccttgc ccacacgccc actgttcccg ataatcgccc caagggtacc
     6661 gcgagagagc cctgggacgg agtgcggcat gcggctgaca ccgaaggagc tggaccggct
     6721 gacggtcttc accctggccg agctggcgcg caagcgccgg gcgcgcgggc tgaagctgaa
     6781 ccaccccgag agcgtggccc tcatctgcga cgagatcttc gaggaggcgc gggccggcgc
     6841 ggcctacgag gcggtcgtca cccacgccgg gcgggtactc agccgcgacg atgtcctccc
     6901 cggcgtgccg gagatgatcc cggtggtgca ggtcgacgcg ctcttccccg acggcacgcg
     6961 gctggtcacc gtgcggaccc cgatccgatg agcggcgagc tgccgcccgg cgcgatcctg
     7021 gcgggcgagg gcaccatcga gccgaacgcc ggactggagc ggcgcgccct gcgcgtcatc
     7081 aacaccggcg aggtgccggt gcagctgacg gcgtatttcc acgtcttcga ggccaacccg
     7141 cggctctgct tcgaccgccg ccgcgccttc ggtatgcgac ctgatgtgcc catcggcggc
     7201 gcggtccgca tcgagcccgg cgcgaccgtc gagctgatcg tcgtcccctt caccggccgc
     7261 cgccagatct acggcttcaa cggcctggtt gacggcccgc tgaacgatgc ggatctcgac
     7321 gcccttctcg cccgcctgat cgaccgcggc tt
//
LOCUS       DASUBI010000819         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_852271, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000819 DASUBI010000000
VERSION     DASUBI010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852271"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VFU72_16115"
     CDS             complement(<1..1650)
                     /locus_tag="VFU72_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5425068.1"
                     /translation="MASRVDLRMNPGQASRNSLGLAFRWLVAFLGGLALLAAFLTLSV
                     RLDANFVLLLILAALADALLQIQLRDGRAVSLASTFTFVLFLQYGAAAAALAHAVGLL
                     VRSGLRRARTGQADTATFTAFNLGQLSLSALGAGLLVRAVLGGGLAQPAADPGLALLI
                     YAPTFLSGNIIFTSAAVWPSHGAEHVRTILWPRTSLWTAVSFCFTAPLALATLGIAHV
                     VPFAVASVLVFFAAAGVHAIVQLNLELGERNQQLARANLDLQTINDAARVLASEIDFS
                     TLGPALHEQVRRLVSTDDFLFARAGGRDYQVEWVAEQDEPRSARPSDRCLLIDHVVRT
                     GDLVVARQPEELRRWGLMGWDTSGAVAGIPLRAAGRTVGAFVVRSQSSAAYNERQLSL
                     LETLAHNAAVSLENAALVEQLRAALRDREEYLSVVSHELKNPIAAIQAYAQLAVRRSS
                     GEDERLQEPLARIAGQTERLRRLVDDLLSLSRIDTGHFDLERAETDLRVLATQAVEAA
                     RQSDHEHRFALCLSETAVPADVDPARLLQVLDNLINNAVKYS"
     gene            complement(1766..>2064)
                     /locus_tag="VFU72_16120"
     CDS             complement(1766..>2064)
                     /locus_tag="VFU72_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5425069.1"
                     /translation="FDLSGGERQRAALAAVLVGAPPIVALDEPTRGMDARRKHELAVI
                     LSELARDGAAVVLATHDVDLVRSCAHRVVLLDRGRVAAQGRIEAVLAADPLLAA"
BASE COUNT          364 a          684 c          734 g          282 t
ORIGIN      
        1 cgaatacttg accgcgttgt tgatcaggtt gtcgagcacc tggagcagcc gggccggatc
       61 aacatcggcc gggacggccg tctccgacag gcaaagcgcg aagcggtgct cgtggtcact
      121 ctgtcgcgcg gcctcgactg cctgggtggc cagtacacgc agatcggtct cggcgcgctc
      181 gaggtcgaag tggccggtgt cgatgcgcga gagggacagc agatcatcga ccaggcgacg
      241 caggcgctcg gtttgaccgg cgatgcgggc gagtggctcc tgcagtcgtt cgtcttcccc
      301 cgaggagcgg cggacggcca gttgggcgta ggcctggatg gcggcgatcg ggttcttcag
      361 ttcgtggctg acgaccgaga gatactcctc gcggtcgcgc agggccgcgc gcaactgctc
      421 gaccagcgcg gcattctcca gtgagaccgc ggcgttgtga gcgagcgtct ccagcaacga
      481 aagctgccgc tcgttgtagg cggcgctgct ctgactgcgg accacgaagg cgccgaccgt
      541 gcgcccggcg gcacgcaagg ggatgccggc gaccgcgccg ctggtgtccc aacccatcag
      601 gccccatcgt cgtagttcct ccggctgccg ggcgacaacc agatctcccg tgcgcacgac
      661 atggtcgatc aggagacaac ggtcgctcgg ccgggccgaa cgtggctcgt cctgctcggc
      721 gacccactct acctggtaat cgcgtccgcc ggcccgcgca aagaggaagt cgtcggtgct
      781 taccaggcgg cgcacttgct cgtgcagcgc cgggccgagc gtggagaaat cgatctccga
      841 ggccagcaca cgagcggcat cgttgatcgt ctgcagatcg agattggcgc gcgccagttg
      901 ctggttccgc tcgcccagct ccagattgag ctgcacgatg gcgtgcactc cggccgccgc
      961 gaagaagacg aggaccgagg cgaccgcgaa tgggaccaca tgggcgatgc cgagcgtggc
     1021 cagggccagc ggggccgtga agcagaagct gacggccgtc caaaggctgg tgcgcggcca
     1081 gagaatcgtg cgcacgtgct cggcgccgtg gctaggccag accgccgcac tggtgaatat
     1141 gatgttgcca ctcaggaaag ttggggcgta gatcaggagc gccaggccgg gatcggcggc
     1201 cggttgggcc agcccaccac ccagcacggc gcgcaccaac agccccgcac ccaacgcgct
     1261 gagcgagagc tggccgagat tgaaggcggt gaaggtggcc gtgtcggcct gaccggtgcg
     1321 ggcgcgccgg agtcccgagc gtaccagcag cccgacggcg tgcgccaggg ccgcggccgc
     1381 cgcgccgtac tgcagaaaga ggacaaacgt aaaggtggag gcgagcgaga cggcgcggcc
     1441 atcgcgcagt tgaatctgca agagcgcgtc ggccagcgcg gccaggatca gcagcaggac
     1501 gaagttcgcg tcgaggcgca ccgagagcgt gagaaaagct gccagtagcg ccaggccacc
     1561 caggaacgcg accagccagc gaaatgccaa cccgagcgag ttgcgcgatg cctggcccgg
     1621 gttcatgcgc agatcgactc gactcgccaa cgcccctccc cggctcgtgg ctcccgtcgt
     1681 ggcccgcacc accaaactgg ggcgtggtcc tgagttgcgg aaagggagcc gaaggatgca
     1741 aatgcaattc ccgcgccagc gcgcgtcagg ccgcgagcag cgggtcggct gccagcacgg
     1801 cctcgatccg gccctgggcg gccacgcggc cccgatcgag cagcacgacc cgatgggcac
     1861 agctccgcac caagtcgacg tcgtgggtcg cgagcacaac cgctgctccg tcgcgcgcaa
     1921 gttcggagag tatgacggca agctcatgct tgcgtcgcgc gtccatgccg cgcgtcggct
     1981 cgtctagcgc cacgatcggc ggggcgccga cgagtaccgc cgccagcgcc gctcgctggc
     2041 gctcgccgcc cgagagatcg aacg
//
LOCUS       DASUBI010000820        10535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_705163, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000820 DASUBI010000000
VERSION     DASUBI010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10535
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705163"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFU72_16125"
     CDS             complement(<1..976)
                     /locus_tag="VFU72_16125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5425070.1"
                     /translation="MKRLCLFTDSLEPSGVGAHVLTLAARLRQRYRVVVSCPRSEGGC
                     ALLARVRALGIDTLELAERADRPALERAFSRCIRTVDIFHVHAGIGWEGIPAVWFARD
                     AGIPVVLRTEHLPYLLTDPDQQTAFEQMTHIVDRIVCVSDGVSASLRAAGTPPGKLAV
                     VRNGIDPLPKPLNRDLARQDLRLPDEARLILTVGRFTEQKGYRFLAEAAAPVVQQCPE
                     VRLLWVGDGPEREPLRRQVDRLGLSEHVHFLGRRDDVPRLLGAADLFVLPSLFEGLPL
                     VVLEAMAAGLPVIGTRVCGTEEAIRDGVTGRLVPPADSRALACALLDAL"
     gene            complement(1128..1841)
                     /locus_tag="VFU72_16130"
     CDS             complement(1128..1841)
                     /locus_tag="VFU72_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5425071.1"
                     /translation="MTENERPDDSPPVGKVDFGSFRRLSPISRSFGFDRGQPIDRYYI
                     ERFLDRYRNDVRGHVLEIGDDGYTRAFGGARVTRSDVLSLTPSHPTVTIVADLARGDD
                     LPGDQFDCFILTQTLQFIYRLEAAMATVYRLLRPGGVLLATFPVISQICRYDMDRWGD
                     YWRFTSASVQRLVGDTFGAEQTQVESWGNVLAATCFLNGLAAEELMPAELDAHDPDYQ
                     VLITARALKTAAGSSPRAG"
     gene            2020..2829
                     /locus_tag="VFU72_16135"
     CDS             2020..2829
                     /locus_tag="VFU72_16135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5425072.1"
                     /translation="MLVDAHLHCSGRERTDEVLRALDEASIERAVLLAPFLTPPYTLE
                     DRAMLRAANEHLATLVRGHLDRLVGFAVVNPLHPEAADDLEYAVERLGLRGLKLVPAG
                     WYPYDDCAHRVYERAAALGIPILFHSGIFIDGRSGRFCRPTFYEALREHPSLRVTLAH
                     LGWPWCDEAIAVGLIDLINGKSPVDCQFRFDLSFGPSPIYRREVLARALAVLGPGLLQ
                     FGSDRFLPCSGAHIREMVDEVERLLDELQVDSEARERVMAGTASAWLGLAT"
     gene            2867..4096
                     /locus_tag="VFU72_16140"
     CDS             2867..4096
                     /locus_tag="VFU72_16140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5425073.1"
                     /translation="MRRDADRGTNPSGLHVLEVVGNSIVGGMERQVIALSSQLRARGI
                     TVTCLCPNESAFTRDMRALGCDVFVTPVRDDPAWRSIQMAVELIRLRGVSLLHAHMAR
                     AHVLAGLAGSLTRIPVVATIHGKDVSSQELGITRTLGSHLIVVCQEAFAQALALGVPP
                     GRVSLIRNGVDTRIFAPGGSGSKFRAALDVPEEAPLVGFVGRLAYEKGPDQFIRLAAV
                     VHGQRPDVHFALVGEGDQRQTVSALIGEHGLGEVVHLAGEWLDMGAVYPALDLVVQTS
                     RSEGLSLSVLEAMASGLPVVATAVGGSAELVEAGITGRLAGAGDWEGAARAVVDLLAR
                     PERLREMGLAACERARAHFNLTTTGQLTAGLFERLSASGFQPAHPWEITLPAAGAASA
                     EEPLPEAERHALGQLGG"
     gene            complement(4112..4780)
                     /locus_tag="VFU72_16145"
     CDS             complement(4112..4780)
                     /locus_tag="VFU72_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU5425074.1"
                     /translation="MEPPAYVTFDCYGTLIDFDLTPVTLARLGSRAAAIDLTAFLQTF
                     EELRFQEVLGPYRPYREILARSLARAMEAFGLRYAPADGAALADALPAFEPFPEVPEV
                     LARLAQRFRLVLVSNTDDDLIAAALPKLRAPFYRVITAEQARAYKPAAAMFAFVLTTL
                     GCTPGDLTHVAQGFRYDIMPTYALGWRRVWINRRHQPGDPAYGPYQELPDLSGVPALL
                     GIED"
     gene            complement(4857..5534)
                     /gene="queC"
                     /locus_tag="VFU72_16150"
     CDS             complement(4857..5534)
                     /gene="queC"
                     /locus_tag="VFU72_16150"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU5425075.1"
                     /translation="MASPAPERKRAVVLLSGGLDSTTAAWLARASNYDLRALSFDYGQ
                     RHRRELEAAARVAERLGARQQVARLNLGDWGGSSLLGAGDIPSQPAGGIPSTWVPARN
                     LIFLSIAAGYAEVVGASAIYIGVSQVDYSGYPDCRAPFLDAFQRTADLASKQFVEQGI
                     SIPVVAPFLHLSKAGIVRLGLTLGVDYALTWSCYQGGERPCGVCDSCRLRAAAFAELG
                     LPDPLLG"
     gene            5599..6237
                     /locus_tag="VFU72_16155"
     CDS             5599..6237
                     /locus_tag="VFU72_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5425076.1"
                     /translation="MADRQGQQQAHEMDGHAPGRLADQVYFGGAFLTALATLAWHRRW
                     AEASDGTPRRGLFDGLCHLGTALAVALPVLPYVRDRAGFLRLALLSAVMIDLDHVAAA
                     RSVRFERTMTMAHRPASHSLAAPLVCAALAERWRPRRHLGLAALLGLGSHLLRDLGTG
                     GAPLVRPSRVVSMPNPVVVFLLGALALTSRTATRLALGAHLPLPLPRGQHTA"
     gene            6234..6602
                     /locus_tag="VFU72_16160"
     CDS             6234..6602
                     /locus_tag="VFU72_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425077.1"
                     /translation="MSDAHGTLGVVLALLYLGIAVAAYLLASRAPESAPLRATLPPWL
                     TGTAHALLGVQVLMGIILFARHPHIMPVSHAVAGILTVVALGLMVPLGRRYGRARGLA
                     YSALLVAILCGVAIVIAFTR"
     gene            6655..7341
                     /locus_tag="VFU72_16165"
     CDS             6655..7341
                     /locus_tag="VFU72_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425078.1"
                     /translation="MGLDVYLADGWRTAYPGASVGILALDGVENPREHAALVERVRRV
                     EAALRQQWAGASRTELNQIPEFDAYRGYYRRFGKTYHVQLQFESVVLKGKPLRANGAL
                     VLAMFAAELGNRLLTAGHDRALIEGGLSIDVADGGQRYLGIGGQELALQPGDMYIRDE
                     AGILSSVLYGPDDRSKITPGTRQALFCVYAPPGIPPEAVASHLADIAGNVRLVAPQAA
                     VTEQRVYTAS"
     gene            complement(7398..7913)
                     /locus_tag="VFU72_16170"
     CDS             complement(7398..7913)
                     /locus_tag="VFU72_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm5 family immunity protein"
                     /protein_id="HEU5425079.1"
                     /translation="MILPKVRDPRFVTIRRGGTLTDADHRLLALWAAACAEHVLDLFE
                     AVRPEDPRPRQAIEQARAWVRGEITMTRARTAAGHANAAARDLSGAARHAADAAAQAA
                     AVAHVAAHELGAAAYAIKAARAAAPKGEDERAGRRECRWQRDQLPQAIRALVLDDQRV
                     RNGICWSVFEC"
     gene            8073..9458
                     /locus_tag="VFU72_16175"
     CDS             8073..9458
                     /locus_tag="VFU72_16175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5425080.1"
                     /translation="MTKDYARYSYWLDTCGDDLTPRAPLDGSIEVDVGILGAGFTGLW
                     TAYHLLRRDPSLKVAVVEAEIAGFGASGRNGGWCFSGFPFPLAKLRAQYGDDAARAVM
                     RQMYASVDNVGAVCREEGIDAHYAKSGTLEIARAPHHLPHLLEWYREYQELGLADHYA
                     LLDVEGTAEYLRVAGMEGSLLIKEGATVHPARLARGLAHAVERHGGTIYEQTRVTDYT
                     PGPRPRLITERGEVSARAIVLAGEAYLAQLPKLQRQIVPMTSHIVITEPLADDIWQEI
                     GWEGRQVIGSSGTMSGYLNKTVDGRIAFGPYRGVYPYGSHISDALDRQEAIFAHGRAA
                     ALDWFPLIRGARFTHAWGGVFGIPRDRMPTMSYDPKTGIATGRGYTGEGVATANLSGR
                     VLADLISGADSELTHLPMTAHHSPDWEPEPLRWLGVNAVRKSLARVERKAEQTGRYPD
                     KPTLAQRIWRR"
     gene            complement(9654..>10535)
                     /locus_tag="VFU72_16180"
     CDS             complement(9654..>10535)
                     /locus_tag="VFU72_16180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5425081.1"
                     /translation="MEQSTRIATELGYLKPPDDVMVPLEVAVKLPREKVLLLTTGSQG
                     EASAALSRIASGEHPEVRLSKGDTVIFSATPVPGNEEDVSETIDLLFKRGIKVIYAAL
                     EPTIHVSGHASREELKLMLSLLRPHFIVPIHGEYRHLVLYEELAISEGYAQSRIFLPE
                     LGGVLGFGRDAGRREGTVAHGAVLVDILGPKNVILRKREEIAQNGFIIATMVLDHESR
                     QLIAGPTLAAENVNGHITNEMLRGAEADLKKFLDRRAKGGFSTGYLVSRTKGVLSRSI
                     YRQSKIHPLIMPVVTEL"
BASE COUNT         1512 a         3651 c         3809 g         1563 t
ORIGIN      
        1 tgagggcgtc gagcagcgcg cacgccagag cgcgcgagtc agcgggtggt acgagccgtc
       61 ccgtgacgcc gtcgcggatc gcctcctctg tcccgcagac gcgcgttccg atcaccggca
      121 gcccggccgc catggcttcc agcacgacga gcggcagccc ctcgaacagc gagggcagca
      181 cgaacaggtc cgcggcgcca agcaagcgcg ggacgtcgtc tcgccggccc aggaagtgga
      241 catgctcaga gagtccaagc cggtctacct gccggcgcag cggctcgcgc tcggggccgt
      301 cgccgaccca gagaagccgc acctccggac actgttgaac caccggggcc gccgcctcag
      361 cgagaaaccg gtaccccttt tgctcggtga agcgcccgac ggtgaggatc agccgggcct
      421 cgtcggggag gcgcaggtcc tggcgggcca ggtcccggtt cagcggcttc ggcagcggat
      481 cgatcccgtt gcgcaccacg gccagcttcc cgggcggcgt accggccgcg cgcagcgacg
      541 cggagacgcc gtccgagacg cagacgatgc ggtcgacgat gtgcgtcatc tgctcgaagg
      601 ctgtctgctg gtccggatcg gtcagcaggt acggcagatg ctcggtgcgc agcacgacgg
      661 ggatgccggc gtcccgcgca aaccagaccg ctgggattcc ctcccagccg atgccggcgt
      721 gcacgtggaa gatgtccacc gtacggatgc aacgcgaaaa cgctcgctcg agcgccgggc
      781 gatccgcccg ctcggccagc tccagcgtgt cgatccccag cgcccggacg cgggcgagca
      841 gcgcgcagcc gccctcgcta cgggggcaag acacgaccac gcggtagcgc tgccggaggc
      901 gggcggcgag cgttagcaca tgcgcgccga ccccggaggg ttccaggctg tcggtgaaca
      961 gacacagccg tttcatcggc ggctcccaag gagagcagca ggtccgcgag cgatcgccgg
     1021 cggctggaga acggggatcg caggagccgc gccgtcgccc cgcggggccg gcgcgagctc
     1081 cggtcttcgt cgcccgattg gccggtccgt gcgtggtcgc cggagtccta gccggcgcgg
     1141 ggactggagc cagcggccgt tttcagcgcg cgggcggtaa tgagcacctg atagtcgggg
     1201 tcgtgggcgt ccagctcggc cggcataagc tcctcggccg cgagaccgtt gaggaagcac
     1261 gtcgccgcca gcacgttgcc ccacgattcc acctgcgtct gctcggcgcc gaaggtgtcg
     1321 ccgaccagcc gctgcaccga ggcggaggtg aagcgccagt agtctcccca gcggtccatg
     1381 tcgtagcggc agatctgact gatgaccggg aaggtcgcca gcaggacgcc acccggccgg
     1441 agcagccgat agaccgtcgc catggccgcc tcgagccggt aaatgaactg cagcgtctgg
     1501 gtgaggatga aacagtcgaa ctggtcaccg ggcaggtcgt cgccgcgggc caggtccgcc
     1561 acgatggtca cggtcgggtg cgaaggcgtc aggctgagca catcggagcg ggtcacccgc
     1621 gcgccgccga aggcgcgcgt gtagccgtcg tcgccgatct ccagcacgtg gccacggacg
     1681 tcgttgcggt agcggtccag gaaccgttcg atgtagtagc gatcgatcgg ctgaccgcga
     1741 tcgaagccga acgaccggct gatcgggctg agacgccgga agctgccgaa gtccaccttg
     1801 ccgaccggcg gcgagtcgtc gggccgctcg ttctccgtca tccggcgcct ccctggtgcc
     1861 gagcgcggta cacagtttgc gtccccgccg gagcgatgac caggccgcgc cgagcgcgcg
     1921 acctgatcca cgatgctcgc cgtttcattc ggtgcgggcg gccacaactc ggcaaggtca
     1981 attccgtacc gagtttggcc ccgcggggga tggtagcaga tgctcgtcga tgcacacctg
     2041 cattgttccg gccgcgagcg caccgatgag gtgctgcgcg cgctcgacga ggcgagcatc
     2101 gagcgggcgg tcctgctggc gccgttcctc acgccgccct acacccttga agatcgcgcg
     2161 atgctgcgcg ccgcgaacga gcacctcgcg actctcgtgc gcggccacct cgaccggcta
     2221 gtcggtttcg ccgtcgtcaa cccgctgcac ccagaggccg ccgacgacct ggagtacgcc
     2281 gtcgagcgcc tcggcctgcg cgggctcaag ctcgtgccgg ccggctggta tccctatgat
     2341 gactgcgccc accgggtcta cgagcgcgcg gcggccctcg gcatccccat cctcttccac
     2401 agcggcatct tcatcgacgg tcgctcgggg cgcttctgcc ggccgacctt ctacgaggcg
     2461 cttcgcgagc atccgagcct gcgggtcacg cttgcccacc tcggctggcc gtggtgcgac
     2521 gaggccatcg ccgtgggctt gatcgacctg atcaatggga agtcgccggt tgactgccag
     2581 ttccgcttcg atctctcatt cgggccatcg ccgatctacc ggcgtgaggt gctggcgcgg
     2641 gcgctcgccg tcctgggtcc gggtctgctg cagttcggca gcgaccgctt cctgccgtgc
     2701 agcggggcgc acatacggga gatggtcgac gaggtggagc ggttgctcga cgagttgcag
     2761 gtcgatagcg aggcgcggga gcgtgtcatg gccgggaccg cctcggcctg gctcgggctg
     2821 gcaacctgag atgaccggtg gctcgcggcg ctgatggagg gtgctgatgc gaagggacgc
     2881 ggatcgaggg acgaacccgt ccgggctcca cgtgctcgaa gtggttggga actcgatcgt
     2941 cggcggcatg gagcggcagg tcatcgccct gtcgagtcaa ctccgcgctc gcggcatcac
     3001 cgtcacctgc ctctgcccga acgagagcgc cttcacgcgc gacatgcgcg ccctgggttg
     3061 tgacgtcttc gtcacccccg tgcgcgacga cccggcctgg cgctcgatcc agatggcggt
     3121 ggagctcatt cggctgcggg gcgtgagcct gctccacgcg cacatggcac gggcacatgt
     3181 gctcgccggc ctggcgggga gcctcacacg aatcccggtc gtggccacca tccacggcaa
     3241 agatgtgtcc tcccaggagc tcggcattac ccgcaccctg ggctcgcacc tgatcgtcgt
     3301 ctgtcaggag gccttcgccc aggcgctggc gctgggcgtg ccgccggggc gcgtatcgct
     3361 gatccgcaac ggagtggaca cccggatctt cgcccccggc ggctccggca gtaagttccg
     3421 cgcggcgctc gacgtaccgg aagaggcgcc cctggtcggc tttgtcggcc ggctggcgta
     3481 cgagaagggc cccgatcagt tcattcgcct ggcggcggtg gtgcatgggc agcggcccga
     3541 cgtccacttc gcgctggtcg gcgagggaga tcagcggcag acggtgtcag cgctcatcgg
     3601 tgagcatggt ctcggcgagg tggtacatct ggccggcgaa tggctggata tgggcgcggt
     3661 ctaccccgcc ctcgacctcg tcgtgcagac ctcacgcagc gagggtctgt cgctctccgt
     3721 gttggaggcg atggcctcgg ggctgccggt cgtggcgacg gcagtgggcg ggagcgctga
     3781 gctggtcgaa gcgggtatca cggggcggct cgcgggcgcc ggcgactggg agggagccgc
     3841 ccgggcggtg gtcgacctgc tcgcgcgccc ggaacgactg cgggagatgg gcctggcggc
     3901 gtgcgagcgc gcgcgggcac acttcaacct cacgactacc gggcagctca ccgccgggtt
     3961 gttcgagcgg ctctcggcgt ccggcttcca gcccgctcat ccgtgggaga tcacgctgcc
     4021 ggcggccggc gctgcctccg cggaagagcc cctgcccgag gcggagcgtc acgcgctcgg
     4081 tcagcttggc ggctagcggc ggctcccgcg ctcaatcctc gatgcccagc agtgccggca
     4141 cgccggataa atccggcaat tcctggtagg ggccgtaggc cgggtcgccg ggctggtggc
     4201 gccggttgat ccagactcgg cgccagccga gcgcgtaggt cggcatgatg tcgtagcgaa
     4261 agccctgcgc cacgtgcgtg aggtcgccgg gcgtgcagcc gagggtcgtg agcacgaacg
     4321 cgaacatggc cgcggccggc ttgtaggcgc gcgcctgctc ggccgtgatg actcgataga
     4381 acggggcccg gagcttcggc agggcggcag cgatcaggtc gtcgtcggtg ttggacacca
     4441 gcacgagccg gaagcgctgc gcgaggcgcg cgagaacctc cggcacctcc ggaaacggtt
     4501 cgaacgcggg cagcgcgtcc gccagcgccg cgccgtcggc gggggcgtag cggagcccga
     4561 atgcttccat ggcccgggcc aggctgcgcg ccaggatctc tcgatagggg cgatacggac
     4621 ccagaacctc ctggaagcgt agctcctcga acgtttggag aaatgccgtg aggtcgatcg
     4681 cggcggcgcg cgagccgagg cgcgccagcg tgactggagt gaggtcaaag tcgatcaagg
     4741 tgccgtagca gtcaaaggtg acgtaggccg gcggttccac gcgggctcct ctcactccgt
     4801 ccagatgccg tcccgctgtg cgtgctgcag gcgcctctgg ctcgtcgcgg accggctcac
     4861 cccagcagcg gatcgggcag acccagctcg gcgaaggcgg ccgcgcgcag ccggcaggag
     4921 tcacagacgc cgcagggccg ctcgccgccc tggtagcagg accaggtcag cgcgtagtcg
     4981 acgcccagcg tcagccccag ccgcacgata ccggctttcg agagatggag gaagggcgcg
     5041 actaccggga tgctgatccc ctgctcgacg aactgcttgc tggccagatc ggcggtgcgc
     5101 tggaaggcgt ccaggaaggg cgcacggcag tcgggatagc cggagtaatc gacctggctg
     5161 acgccgatat agatggcgct ggctccgacc acctcggcgt agccggcggc gatcgagagg
     5221 aagatcaggt tgcgggccgg tacccaggtc gaagggatgc cgcccgccgg ctggctgggg
     5281 atgtcgcccg cgcccaggag cgacgagccg ccccagtcgc ccaggttgag gcgcgcgacc
     5341 tgctgccggg cgccgagccg ctcggctacg cgtgcggccg cctccagctc gcggcggtgg
     5401 cgctggccgt agtcgaagct gagggcgcgg aggtcgtagt tgctggcccg cgccagccag
     5461 gcggcggtgg tgctgtccag cccgccggaa aggaggacga ccgcgcgctt gcgttccggt
     5521 gcgggacttg ccatgctgta gcacaatcct ctcgcgtgag ggccgatggc gggagcgatc
     5581 gatcggagtg gacgagcgat ggccgacagg caggggcagc agcaggcgca cgagatggac
     5641 ggtcacgcgc cgggccggct ggccgatcag gtgtacttcg gcggcgcgtt cctgaccgcg
     5701 ctggcgacgc tggcctggca ccgccgctgg gcggaggcca gcgacggcac cccgcggcgc
     5761 ggcctcttcg acggcctctg ccacctcggc accgcgctgg cggtggcgct gccggtgctg
     5821 ccctacgtgc gcgatcgggc cggcttcctg cgcctggcgc tgctgagcgc ggtcatgatc
     5881 gacctcgacc acgtggcggc cgcacgctcg gtgcgcttcg agcgcaccat gacgatggcg
     5941 caccggccgg cctcgcactc actggcggcg ccgctcgtct gcgccgcgct ggccgagcgc
     6001 tggcgcccgc ggcggcatct cgggctggcg gcgctgctcg ggctcggctc gcacctgctg
     6061 cgcgacctcg gcacgggcgg cgcgccgctg gtccgtccga gccgggtcgt ttcgatgccc
     6121 aacccggtcg tcgtcttcct gcttggggcg ctggcgctga ccagtcgcac cgcgacgcgg
     6181 ctagcgctcg gcgcgcacct gcccctgccg ctcccgcgtg gtcagcatac ggcatgagcg
     6241 acgcacacgg caccctgggc gtcgtgctgg cgctgctcta cctcggcatc gcggtagccg
     6301 cctacctgct ggccagccgc gcgcccgaga gcgcgccgct gcgcgccacg ctgccgccct
     6361 ggctgaccgg cacggcgcac gcgctcctgg gcgtgcaggt gctgatgggg attatcctct
     6421 tcgcgcgtca cccccacatc atgcccgtct cgcacgccgt cgcgggcatc ctgaccgtgg
     6481 tggcgctggg gctgatggtg ccgctcggcc ggcgctacgg ccgggcgcgc gggctggcct
     6541 acagcgcgct gctggtcgcc atcctctgcg gcgtcgccat cgtcatcgcg ttcacgcgct
     6601 agtcgggcgc ggcgtgagac aaccggggga gagcgcatag ggaggctgca cggcatgggc
     6661 ctggacgtgt acctggcgga cggctggcga acggcctatc ccggcgccag cgtcggcatc
     6721 ttggcgctcg acggagtcga gaatccgcgc gagcacgccg cgctggtcga gcgggtccgg
     6781 cgcgtcgagg ccgcgctgcg gcagcagtgg gccggcgcca gccggacgga actgaaccag
     6841 atccctgagt ttgacgccta tcgcggctac taccggcgct tcggcaagac ctaccacgtc
     6901 caactgcagt tcgaatcggt cgtgctcaaa ggcaagccgc tgcgcgccaa tggggcgctc
     6961 gtgctggcga tgttcgccgc cgagctgggc aaccggctgc tgaccgccgg gcacgaccgc
     7021 gcgctgatcg agggtggcct gtcgatcgat gtggccgacg gtggccagcg ctacctgggc
     7081 atcggcggcc aggagctggc cttgcagccg ggcgacatgt acatccgcga cgaggcaggc
     7141 attctctcca gcgtgctcta cggccccgac gatcgctcca agatcacgcc gggcacccgg
     7201 caggccctct tctgcgtcta cgcacccccc ggcatcccgc ccgaggcggt cgccagtcac
     7261 ctggccgata tcgccggcaa tgtccgcctg gtcgcgccgc aggcggccgt caccgagcaa
     7321 cgggtctaca ccgcgagcta agcgcacggc gtctgcccgc tgccctcgtg aagcgtggtc
     7381 ggcaccccgg cgcgcgctca acactcgaac accgaccagc agattccatt gcgcacgcgc
     7441 tggtcgtcga gtacgagcgc gcgaatcgcc tgcgggagct ggtcgcgctg ccagcggcac
     7501 tcgcgccgcc ccgcgcgctc atcttctccc tttggcgcgg cggcgcgcgc ggccttgatc
     7561 gcgtaggcgg ccgcgccgag ctcgtgcgcg gcgacgtgcg cgacggccgc ggcctgggcg
     7621 gcggcgtcgg cggcatgccg cgccgccccg ctgaggtcgc gggccgcggc gttggcatgg
     7681 cccgcggccg tgcgcgcccg ggtcatcgta atctcgccac gcacccaggc gcgggcctgc
     7741 tcgatcgcct ggcgcggcct cgggtcctcg ggccggaccg cctcgaagag gtcgagcacg
     7801 tgctccgcgc aggccgccgc ccacagggcg aggagccggt gatccgcatc ggtgagcgtc
     7861 ccgccgcggc gaatcgtcac gaaacgggga tcgcggactt tcgggagaat cacagccggc
     7921 acccccttat agaacctggt ctggactatt cgacacggaa ttgtgccagt cgcgggtcgc
     7981 gccgggagcg cgacggcctg cgtggacttg gcagaatcgg ggatgagccg catccttggc
     8041 gcgcggacac cagcggagac gaggcgcgag gcatgacgaa ggactacgcg cgctatagct
     8101 actggctcga cacctgcggc gacgacctga cgccgcgcgc gccgctcgac ggctcgatcg
     8161 aggtcgacgt cggcatcctc ggcgccggct tcaccggcct gtggaccgcc taccacctgc
     8221 tcaggcgcga tccgtcgctg aaggtcgccg tggttgaggc ggagatcgcc ggcttcggcg
     8281 cctcgggccg caacggcggc tggtgcttct ccggcttccc cttcccgctg gcaaagctgc
     8341 gcgcccagta tggcgacgac gcggcccgcg ccgtcatgcg ccagatgtac gcgtccgtcg
     8401 acaacgtcgg cgcggtctgc cgggaagagg ggattgacgc ccactacgcc aagagcggca
     8461 cgctggagat cgcccgggcg ccgcaccatc tgccccacct gctggagtgg taccgcgagt
     8521 accaggagct ggggctggcg gaccactacg cgctgctcga cgtcgagggg accgccgagt
     8581 acctgcgcgt ggccggcatg gaggggtcgc tcttgatcaa ggagggtgct acggtccacc
     8641 ccgcgcggct ggcgcgtggc ctggcccacg cggtcgagcg ccacggcggc acgatctacg
     8701 agcagacgcg cgtgaccgac tacacgccgg gcccgaggcc gcggctgatc acggagcgtg
     8761 gcgaggtgtc cgcgcgggcc atcgtgctgg ccggcgaggc ctatctggcg cagctgccga
     8821 aattgcagcg ccagatcgtg ccgatgacct cgcacatcgt catcaccgag ccgctggcag
     8881 acgacatctg gcaggagatc ggctgggagg ggcgccaggt gatcggcagc tcgggcacga
     8941 tgtcgggcta tttgaacaag acggtcgacg gccgcatcgc cttcggtccc taccgcggtg
     9001 tctaccccta cggctcgcac atctccgacg cgctcgaccg gcaggaggcg atcttcgccc
     9061 acgggcgcgc ggcggcgctc gactggttcc cgctgatccg cggcgcgcgc ttcacccacg
     9121 cctggggcgg cgtcttcggc atcccgcgcg accgcatgcc gaccatgtcc tacgatccga
     9181 agaccggcat cgcgaccggc cgcggctaca ccggcgaggg cgtagcgacc gcgaatctct
     9241 ccggccgcgt gctggccgac ctgatcagcg gcgccgacag cgagctgacg cacctgccga
     9301 tgacggcgca ccactcgccc gactgggagc cggagccgct gcgctggctc ggcgtgaacg
     9361 cggtgcgcaa gagcctggcg cgggtggagc gcaaggcgga gcagacgggg cgctaccccg
     9421 acaagccgac cctggcccag cgcatctggc ggcggtgagg ggtttgcgtt gcccgcgagc
     9481 ctggcggaat cgcgcacgcc ggtagggcag gcccctgtgc ctgccctcgc tcgatgcccg
     9541 ccggacgtgg tcgacggccg cggcgttgca ccaattcccc gttgccgatg cgtaggggaa
     9601 gcagggcaga cacggaggtc tgcccctaca agggaacgct tggtgccgat ccgctacaac
     9661 tccgtcacca ccggcatgat cagggggtgg atcttcgact ggcggtagat cgagcggctg
     9721 agcacgcctt tggtgcggct gaccagatag ccggtgctga agccgccctt ggcgcggcgg
     9781 tcgaggaact tcttcaggtc ggcctcggcg ccgcgcagca tctcgttggt gatatggccg
     9841 ttgacgttct ccgccgccag cgtcggcccg gcgatcaact ggcgcgactc gtggtcgagc
     9901 accatggtgg cgatgatgaa gccgttctgg gcgatctcct cgcgcttgcg cagaatcacg
     9961 ttcttgggtc cgagaatgtc gaccagaaca gcgccgtgcg ctaccgtgcc ctcgcgccgg
    10021 ccggcgtcgc ggccgaaacc gagtacgccg cccagctcgg ggaggaagat gcgcgactgg
    10081 gcgtagccct cgctgatcgc cagctcctcg tagagcacca ggtggcgata ctcgccgtgg
    10141 atcggcacga tgaagtgcgg gcgcaggaga ctgagcatca gcttcagctc ctcgcggctg
    10201 gcgtggcccg agacgtggat ggtcggctcc agcgcggcgt agatcacctt gatgccgcgc
    10261 ttgaagagga ggtcgatcgt ctcggacaca tcctcctcgt tgcccggcac cggggtggcc
    10321 gagaagatga ccgtatcgcc cttgctcagg cgcacttcag ggtgctcgcc gctggcgatg
    10381 cgcgagagcg cggcgctggc ctcgccctgg ctgccggtgg tcagcagcag caccttctcg
    10441 cgcggcagct tgaccgccac ctccagcggc accatcacgt cgtcgggcgg cttgaggtag
    10501 cccagctcgg tcgcgatgcg cgtgctctgc tccat
//
LOCUS       DASUBI010000821         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1025156, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000821 DASUBI010000000
VERSION     DASUBI010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025156"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFU72_16185"
     CDS             complement(<1..531)
                     /locus_tag="VFU72_16185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5425082.1"
                     /translation="MDQAVHPAVETLDPYAFLAVVGKRVIHPGGRRSSDELLQRGEFG
                     AGQRVLDVGCGVGTTAIAIARCFDATVSAVDISPVMLTRARANVRAAGLGERVSVEEG
                     DILALPFPVSSFQRVVAEAVTMFVDRPRAARELVRVCAPGGKVLATEFYWRAQPTPEA
                     RQIFLGEVCPGMQFDSA"
     gene            complement(536..703)
                     /locus_tag="VFU72_16190"
     CDS             complement(536..703)
                     /locus_tag="VFU72_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425083.1"
                     /translation="MTGIIAAAGFEAIRFSKERYDVFSDAPSASSAKEFGTRGVNFSA
                     RKPALVETMGG"
     gene            complement(731..1336)
                     /locus_tag="VFU72_16195"
     CDS             complement(731..1336)
                     /locus_tag="VFU72_16195"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5425084.1"
                     /translation="MPGTMAVDLTALRTAIQDEYADVAACPTKGFHFHTGRFLARRLG
                     YDPRQIDDLPDAIVESFAGVGNPLALGSLHGGETVLDLGSGAGFDALLAARLVGPAGR
                     VVGVDMTPAMLAKARANAELLGLDNVEVRHGFAESLPLPDASVDVVISNGVINLCPDK
                     RAVFAELFRVLKPGGRLQLADIVVQTAVPEDAKADISLWTG"
     gene            1510..>2101
                     /locus_tag="VFU72_16200"
     CDS             1510..>2101
                     /locus_tag="VFU72_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5425085.1"
                     /translation="MNKRAFKDQLYAEFARIAKAMANPHRLELLDLLAQGERSVEELA
                     QEAGLSLANASAHLQILRRAHLVETEKRGLNVIYCLAAPEVYALWRTLRDLGTAQLAE
                     IDRLVSAFFREREQFEAIGKEELRSRLAAGDVVVLDVRPVLEYAQGHLPGAVSIPIQM
                     LEQRLSDLPRDREIVAYCRGPYCVYADEAVHLLHEHG"
BASE COUNT          336 a          727 c          713 g          325 t
ORIGIN      
        1 ggccgagtcg aattgcatcc ccgggcagac ctcgcccaga aagatctggc gcgcctccgg
       61 tgtgggctga gcgcgccagt agaattccgt ggccagcact ttcccaccgg gcgcgcacac
      121 ccgcaccagc tcgcgcgccg cccgcggccg atcgacgaac atggtcaccg cctcggcgac
      181 gacgcgctgg aagctgctca cggggaaggg cagcgccaga atgtcgccct cctcaaccga
      241 gacgcgctcg ccgagcccgg cggcccggac gttggctcgg gcgcgcgtca gcatgaccgg
      301 cgagatatcg accgcgctca ccgtggcgtc gaagcagcgc gcgatcgcga tggcggtggt
      361 gcccacgccg cagccgacat cgagcacgcg ctggcccgcg ccaaactcgc cgcgctggag
      421 gagctcgtcg ctcgagcgcc ggccgccggg gtgaatcacg cgcttgccga cgaccgccag
      481 gaaggcatac gggtcgaggg tctcgaccgc cgggtgaact gcctggtcca tgccgctacc
      541 ctcccatggt ctccacgagc gccggcttcc gcgcgctgaa gttcacgccg cgcgtgccga
      601 actccttggc gctcgaggcg ctgggcgcgt cgctgaagac gtcgtagcgt tccttggaga
      661 agcggatcgc ttcgaagccg gccgcggcga tgatcccggt caactctgcc tccagcagag
      721 cgccggcaat tcagcccgtc cagagactaa tatccgcctt cgcgtcctcc ggcaccgcgg
      781 tttgcacgac gatgtcggcg agttggagcc gcccgcccgg cttgagcact cggaagagct
      841 cggcgaagac ggcgcgcttg tccgggcaca aattgatgac gccgttggag atcacgacgt
      901 ccacactggc atcggggagc ggcagtgact ccgcgaagcc gtggcggacc tcgacattgt
      961 cgagtccgag gagctcggca ttggcccgcg ccttggcgag catggccggc gtcatatcga
     1021 cgccgacgac gcgccctgcc ggcccaacca ggcgtgcggc cagcagcgcg tcgaatccgg
     1081 cgccggagcc aaggtcgagg acggtctccc ctccatgcag cgatccgagc gcgagcgggt
     1141 tgccgactcc ggcgaacgac tcgacgatcg cgtccgggag gtcgtcgatc tgccgcgggt
     1201 cgtagccgag acgccgcgcc aggaagcggc cggtgtgaaa gtggaagccc ttggtcgggc
     1261 atgccgccac gtcggcgtat tcgtcttgga tcgccgtgcg gagcgcggtg agatcgacgg
     1321 ccatcgtgcc gggcatcctg accctccttg taccgggtgg cagacgcgcg acggcgcttt
     1381 ccgctccggc tctcccggtt cggcggccgc ctcgccgccg tcttgacaac cgctggtgaa
     1441 gacagcatac tgccaagtag tcggccagtc aatagatttg ttgaattgtc gcatacaagg
     1501 gtggcagcgg tgaacaagcg cgcgttcaag gaccagctct atgcggagtt tgcccgcatc
     1561 gccaaggcga tggcgaaccc ccaccggctc gagctgcttg atctcctcgc gcagggcgag
     1621 cgcagcgtcg aggagctggc gcaggaggct ggcctcagtc tggccaatgc ctccgcgcac
     1681 ctccagattt tgcgacgcgc gcacctggtg gagaccgaga agcgcggcct taatgtcatc
     1741 tactgcctcg ccgcgccgga ggtctacgcg ctctggcgca cactgcgtga tctcggcacc
     1801 gcccagctcg ccgagatcga ccggctggtc agcgcgttct ttcgcgagcg cgagcagttc
     1861 gaggccatcg gcaaagagga gctgcgcagt cgcctggcgg cgggcgatgt ggtggtgctg
     1921 gatgtccgcc cggtgctcga atacgcgcag ggacacctgc cgggcgccgt ctccatcccc
     1981 atccagatgc tcgagcagcg gctctccgat ctgccgcggg accgcgagat cgtcgcgtac
     2041 tgccgcggcc cctactgtgt ctacgccgac gaagcggtgc acctgctgca tgagcacggg
     2101 c
//
LOCUS       DASUBI010000822         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_771013, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000822 DASUBI010000000
VERSION     DASUBI010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771013"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFU72_16205"
     CDS             <1..390
                     /locus_tag="VFU72_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5425086.1"
                     /translation="NLKRSVVSARVETAAGPLWIYGTHLDNPVGATQVRLDQTRELIR
                     FWNKRSPALLLGDLNADPGDPVFGLFDGAGLVDLGRQLPPGSSTSSDARLIDHILATP
                     DLTLQSIHVPDTWASDHRPLVASISLP"
     gene            438..623
                     /locus_tag="VFU72_16210"
     CDS             438..623
                     /locus_tag="VFU72_16210"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU5425087.1"
                     /translation="MKVREVLDRLLQEGWRIVRQEGSHRQLRKEGNPWVITVPGKLND
                     NLGHGIVNDIRRKAGWK"
     gene            620..850
                     /locus_tag="VFU72_16215"
     CDS             620..850
                     /locus_tag="VFU72_16215"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5425088.1"
                     /translation="MSETAEFTIILEPTETGFSAYSPDVEGVYATGRTVEETISEMRD
                     AIAFHLAGLTDEEKAYHVHPSIVRRVRVPVGA"
     gene            852..1295
                     /locus_tag="VFU72_16220"
     CDS             852..1295
                     /locus_tag="VFU72_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5425089.1"
                     /translation="MIITPFGRRPLDADAAYLVLVQRFPLRPLRSEHDLDRAVALIDE
                     LLDRPDLTASEQDYLSVLGDLVEAYEDKHVAMPDASGVDVLRHLMAEHNLRQIDLAPL
                     FGSKSIVSEVLSGKRPLALSHIRKLSERFGVPADVFIANEDRSSL"
     gene            complement(1290..2786)
                     /locus_tag="VFU72_16225"
     CDS             complement(1290..2786)
                     /locus_tag="VFU72_16225"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU5425090.1"
                     /translation="MAAPLRVALAELSHETNTFSSVPTDLAAFERAGVKRGATLEDLR
                     GTNTAVAGFLDGAAAHGFEVVPLLAVWATPSGLVSRAALETLSGELAAGLAAAGPLDG
                     VLLGLHGAMVAEGAPDADGYILERVRAAVGPDVPVVATLDLHANISERMVALADVLVG
                     FDTYPHVDQRQRGAEAAAILAALLAGELHPTPALLKPPMLPTSQNMITAREPMRSIFD
                     RAHELELVRGVVNITVAGGFPPADVAEAGFSVLVTTDDQPELAQRLAEELATVAWERR
                     AGFLGGVHTWEEAIEALQHVEHGPLVLVDIADNPWTGGPGDGTELLRFLLQQDVHDAA
                     LALIKDPESVRQCIVAGVGNEVQLHLGGKTDRRHGEPLLLTGRVRVIADGRYINAGPM
                     HAGVEVDLGRVIVLVVNGIEVLVCERAETPIDLNVFRAFGIEPTARKVIALKGKGHFR
                     AAFEPIAERVVLVEGPGITGSDLSRLDFRHLRRPIWPLDEAEWPEAQS"
     gene            complement(2786..3619)
                     /locus_tag="VFU72_16230"
     CDS             complement(2786..3619)
                     /locus_tag="VFU72_16230"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEU5425091.1"
                     /translation="MDKLIISVATTGSRTSRQQTPYVPITPEEIAEEAIRCYDEGAAI
                     VHIHVRDDEGRVTCDPARYERVRRIVEERGCPIIVNMSTGGGAGIVSDEERMAPVRLR
                     PEIASFDAGSTNFGAGVFVNSPAFLDTLAERMQEYGVKAEIECFDTGMIENAKRQIAQ
                     GRIEPPYWFQFVLGVRGGAPATAQQLVYMVSQLPPESKWSVCAIGRDQLPLNALAIAM
                     GGHARTGLEDNIYYGYRVLAEGNAPLVARVARLARELQREIASPDEARQLLGLPPRER
                     A"
BASE COUNT          566 a         1291 c         1247 g          547 t
ORIGIN      
        1 aacctcaagc gctccgtggt ctcggcgcgc gtcgaaaccg ccgccgggcc gctctggatc
       61 tacggcaccc acctcgacaa cccggtgggc gcgacccagg tacggctgga ccagacgcgc
      121 gagctgatcc gcttctggaa caagcgcagc ccggcgctgc tgctcggcga cctcaacgcc
      181 gaccccggcg acccggtctt cggcctcttc gacggcgccg ggctggtcga cctcggccgg
      241 cagctcccgc cgggctcgtc tacctcctcc gatgcgcgcc tgatcgacca catcctggct
      301 acgcccgacc tcacgctcca gtcgatccac gtccccgaca cctgggcctc cgaccaccgc
      361 ccgctggtgg cgagcatctc attgccttag catttgacca acggcatatg ggaagagtgg
      421 agtcacgcgt gccgaccatg aaagttcggg aagtgctgga ccgactactg caggaaggct
      481 ggcggatcgt tcgccaagaa gggagccacc gccagcttcg caaagaaggg aatccttggg
      541 taatcacggt gccggggaag ctcaacgaca acctcgggca tggtatagtc aacgacattc
      601 gtcgaaaggc gggctggaag tgagcgaaac ggccgagttc accatcatcc tggaaccgac
      661 cgagacgggg ttctcagcct actcacccga tgtcgagggt gtctacgcca ccggacgcac
      721 tgtcgaggag accatctcgg agatgcgcga tgctatcgcg ttccatctcg caggcttgac
      781 ggacgaagag aaggcgtacc atgtccatcc ctcgatagtg cggcgagtcc gagttcccgt
      841 cggcgcctga tatgataatc acgccgttcg gaaggcgccc gcttgacgcc gacgcggcct
      901 atctcgtcct ggtgcagcgg ttcccactgc ggccactccg cagcgagcac gacctcgatc
      961 gggcggtcgc gctgatcgac gagttgcttg atcggccgga cctgaccgct tcggagcagg
     1021 actacctcag cgtcctcggt gatttggtcg aagcctacga ggacaagcac gtcgccatgc
     1081 cagatgccag cggcgttgac gtcctgcggc atctgatggc agagcacaac ctgcgccaga
     1141 tcgacctcgc cccgctcttc ggcagcaagt cgatcgtctc cgaggtcctg agcggcaagc
     1201 ggccgctggc gctcagtcac atccgcaagc tgagcgagcg cttcggcgtg ccggctgatg
     1261 tgttcatcgc gaacgaagac cgttccagct tatgactgag cctccggcca ctcggcctcg
     1321 tcgagcggcc agatcgggcg gcgcaggtgg cggaagtcga ggcgcgagag gtcggagccg
     1381 gtgatgccgg ggccctcgac caggaccacg cgctcggcga tcggctcgaa ggcggcgcgg
     1441 aagtgcccct tgcccttgag cgcgatgact ttgcgcgcgg tcggctcgat gccgaaggca
     1501 cggaagacgt tgaggtcgat cggcgtctcg gcgcgctcgc agaccagcac ctcgatcccg
     1561 ttcaccacca gcacgatgac tcgccccagg tcgacctcga ccccggcgtg catcggcccg
     1621 gcgttgatgt agcggccgtc ggcgatcacg cgcacccggc cggtcagcag cagcggctcg
     1681 ccgtggcgcc tgtcggtctt gccgcccagg tggagctgca cctcgttgcc cacccccgcc
     1741 acgatgcact gccgcaccga ctccgggtcc ttgatcagcg ccagcgcggc gtcgtggacg
     1801 tcctgctgca agaggaagcg cagcagctcc gtgccgtcgc cggggccgcc ggtccagggg
     1861 ttgtcggcga tgtcgaccag caccagcgga ccatgctcga cgtgctggag ggcctcgatc
     1921 gcctcctccc aggtgtgcac cccgccgagg aagccggcgc gacgctccca ggccacggtc
     1981 gccagctcct cggccaggcg ctgggccagc tcgggctgat cgtcggtcgt caccagcacg
     2041 ctgaagccgg cctcggccac gtcggcgggc gggaagccgc cggccacggt gatgttgacc
     2101 acgccgcgca ccagctccag ctcgtgggcg cggtcgaaga tcgagcgcat cggctcgcgc
     2161 gcggtgatca tattttgcga ggtcggcagc atcggcggct tgagcagcgc gggcgtggga
     2221 tgaagctctc ccgccagcaa cgcggccagg atcgcggccg cctcggcgcc gcgctggcgc
     2281 tggtcgacgt gggggtaggt gtcgaagccg accagcacgt cggccagcgc gaccatgcgc
     2341 tcgctgatgt tggcgtgcag gtcgagcgtg gccaccaccg gcacgtccgg cccgaccgcc
     2401 gcgcgcaccc gctccagaat atagccgtcg gcgtcgggcg cgccctcggc caccatcgcg
     2461 ccgtgcagcc ccagcagcac gccgtcgagc ggacccgccg ccgcgagccc ggccgccagc
     2521 tcgccgctca gcgtctccag cgccgcgcgg ctgaccaggc ccgagggggt agcccagacc
     2581 gccagcagcg gcacgacctc aaagccgtgc gcggccgcgc cgtcgaggaa gccggccacg
     2641 gcggtgttgg tgccgcgcag atcctccagg gtggcgccgc gcttgacccc ggcgcgctcg
     2701 aaggcggcca gatcggtcgg cacgctgctg aaggtgttgg tctcgtgcga cagctcggcc
     2761 agcgccacac ggagcggggc ggccatcagg cgcgctcccg cggcggcagg ccgaggagct
     2821 ggcgcgcttc atccgggctg gcgatctcac gctgcagctc gcgcgccagc cgggccaccc
     2881 gcgccaccag cggcgcgttg ccctcggcca gcacccggta gccgtagtag atattgtcct
     2941 ccagcccggt gcgggcgtgc ccgcccatgg cgatcgccag cgcgttcagc ggcagttggt
     3001 cgcggccgat ggcgcagacc gaccacttcg actcgggcgg gagctgactg accatgtaga
     3061 cgagctgctg ggcggtggcc ggcgcgccgc cgcgcacccc gagcacgaac tggaaccagt
     3121 agggcggctc gatgcgcccc tgggcgatct ggcgcttggc gttctcgatc atgccggtgt
     3181 cgaaacactc gatctccgcc ttgacgccgt actcctgcat acgctcagcc aaggtatcga
     3241 ggaaggcggg cgagttgacg aagacgcccg cgccgaagtt ggtcgagccg gcgtcgaagg
     3301 aggcgatctc gggccgcagc cgcaccggcg ccatgcgctc ctcgtcgctg acgatgccgg
     3361 caccgccgcc ggtcgacatg ttgacgatga tcgggcagcc gcgctcctcg acgatgcggc
     3421 gcacgcgctc gtagcgcgcc gggtcgcagg tgacgcgccc ctcgtcgtcg cgcacgtgga
     3481 tgtggacgat ggcggcgccc tcgtcgtagc agcggatcgc ctcctcggcg atctcctcgg
     3541 gcgtaatcgg gacgtagggg gtctgctgcc ggctggtgcg gctgccggtg gtagcgaccg
     3601 agatgatcag cttgtccatg gacctggctc ctctccacgg ggcacggggc g
//
LOCUS       DASUBI010000823         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_697199, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000823 DASUBI010000000
VERSION     DASUBI010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697199"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..471)
                     /locus_tag="VFU72_16235"
     CDS             complement(202..471)
                     /locus_tag="VFU72_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425092.1"
                     /translation="MDTISRPLSVLLFSSSNSEATFLAAGLLYQRTDAVWPVLIQGTG
                     DAALSPDVLTVLAEEGSVVAGWVPPVVDVPPTAGVDVGLTICVPT"
     gene            complement(471..1283)
                     /locus_tag="VFU72_16240"
     CDS             complement(471..1283)
                     /locus_tag="VFU72_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5425093.1"
                     /translation="MCVVRSNWITTGRIGGRMRKLGRRTRPCVGEDTLSPKLTARAQV
                     AMTQAHKEAMRRGDDYIGTEHLLLGLLGEAEGIAATVLRGSGFALEATRAAVDRIIGQ
                     ATNPMTEKRALKVTPRLKRVLELAGEEARDFGQRYIGTEHLLIALLREGDGVGADILL
                     GGDLDLDHAREAVRVSLARIGQPAARSEELETSTVVMCRLDAHTLAAVDTLIEAGVRS
                     TRSDAVAWLVQAGIEANTPLLATVQETVTEIRRLRSQAQDLARQALAHDGAR"
     gene            1400..2980
                     /locus_tag="VFU72_16245"
     CDS             1400..2980
                     /locus_tag="VFU72_16245"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEU5425094.1"
                     /translation="MLGPPKGRCLDRPVLVSLEGLVPAGHFYRHLEAALDLDVVREWV
                     AGCYAERGRPSIDPVTFFKLQLIMFFEGLRSERRLLETASLNLAHRWYLGYHLDEPLP
                     DHSSLTRIRERYGLDVFRHFFDHVVQRCVDAGLVWGEEVFFDATKVRANAALDSLAPR
                     LREVVDGHLATLFPAAPAAAAQAAAMEGNPAPAPVAAAPGTAGAPLPAFPSASPSPAN
                     ATPGEQPAPAAARWALLEACRLDPDRPAARGYERTSDWRVSTTDPDAALMKAAGQRPA
                     LGYHDHYVVDGGKARIILHALITPADVMENEPMLDQFRRIQFRWHLRPKRVVADTTYG
                     TAENIGALEQAGIRAYVPLPDCEGRTPYFGSGRFTYAPADDAYCCPAGQPLRRRKAKY
                     TEGVVVYRAEAATCNACALKGTCTPSDHGRTIHRSFHADALERVRGYHATAAYQRAMR
                     KRKVWPEPLFAEAKQWHGLRQFRLRGLQNVNMEGLLVAAGQNLKRWLSATGWGRRHGP
                     TGSLRVARLTIASVPEPR"
BASE COUNT          511 a         1189 c         1011 g          530 t
ORIGIN      
        1 ataataaccg ccacatctcc gctgtcgagc gggccgttat tgcccgctcg acagcaattc
       61 gttggttatc agcgcccgga ggtcgtcgcg gacctggcga tacctcgcca agcgcgccgt
      121 atggttacca ctcccttcgg ccgggttctc aacgcaccac accaggcgcc gaccggcgcc
      181 tctgaccacc ggtcaggtgt ctcaggtcgg cacgcagatg gtcagcccga catcgacgcc
      241 cgcagttggt ggtacgtcaa cgaccggagg gacccagcct gccacgacac ttccctcttc
      301 agcgagtacg gtgagcacgt ccgggctgag cgccgcgtct cccgttccct gaatcaggac
      361 cggccagaca gcatcggtcc gctggtacaa gaggcccgca gcaaggaacg tcgcctccga
      421 gttgctgctg gagaacagca ggacgctcag cggtcggctt atcgtgtcca tcaccttgct
      481 ccatcgtgcg ctaaggcttg ccgtgccagg tcttgcgcct gcgagcgcaa ccgacgaatc
      541 tccgtcaccg tctcctgcac ggtcgccagg agcggcgtat tcgcctcgat gccggcctgg
      601 acgagccagg ccaccgcgtc gctgcgcgtt gagcgcaccc ccgcttcgat gagcgtatcc
      661 accgccgcca gcgtgtgcgc gtcgaggcgg cacatgacga cggtgctggt ctccagttcc
      721 tccgaccgcg cggccggttg gccgatgcgg gcgaggctca cccggactgc ttcgcgggca
      781 tggtcgaggt cgaggtcgcc accgagcaag atatctgccc cgaccccgtc cccttcgcgc
      841 aagagggcga tgaggagatg ctccgtccca atataccgct gcccgaaatc gcgcgcctct
      901 tcgccggcca attcgagcac gcgtttgagg cgcggggtga ccttcaaggc ccgtttctcc
      961 gtcatcgggt tcgtagcctg cccgattatc cggtcaacgg ccgctcgcgt cgcctccagc
     1021 gcgaaccccg acccacggag cactgtcgcg gcgatgccct ccgcttcgcc caagagtccg
     1081 agtaagagat gttccgtgcc gatgtagtcg tcgccgcggc gcatggcctc cttatgcgct
     1141 tgggtcatcg caacttgggc gcgggcggtg agtttcgggg agagcgtatc ctccccaaca
     1201 caggggcgag tccgacgtcc tagctttcgc atgcgacccc caatcctgcc cgttgtaatc
     1261 caattacttc ttacaacgca catggccagg cgaggttacg cgcggctgcg gtctcgttcg
     1321 cataacgcct cagcctgttg aaaatcgctg tgtccagtcg tagcatcccg gccttactcc
     1381 ggctacgggg gagggtgcga tgctggggcc accaaaggga cgctgtctgg atcggccggt
     1441 gctggtctcg ctggaagggc tcgtacccgc gggccacttc taccgccacc ttgaggccgc
     1501 gctcgacctc gacgtcgtgc gcgagtgggt cgcggggtgc tacgccgagc gcggccggcc
     1561 gagcatcgac ccggtcacct tcttcaagct gcaactcatc atgtttttcg agggcctccg
     1621 ctccgagcgc cgcctcctgg agacggccag cctcaatctc gcgcaccgct ggtacctcgg
     1681 ctaccacctg gacgagccgc tgcccgacca ctccagcctg acccgcatcc gcgagcgcta
     1741 cggtcttgac gtcttccggc acttcttcga ccacgtcgtc cagcgctgcg tcgatgccgg
     1801 gctcgtgtgg ggcgaggaag tcttcttcga cgcgacgaag gtgcgcgcca acgcggccct
     1861 ggactcgctt gccccgcgcc tgcgcgaggt cgtggacggc cacctggcga ccctgttccc
     1921 cgccgcaccc gccgctgcgg cgcaggcggc agcgatggag ggcaaccccg cgccggcacc
     1981 tgtggccgcg gcgccaggca cggctggcgc gccactcccc gccttcccta gcgcgtcccc
     2041 gtcgccagcc aacgcgacgc ccggggagca gccggccccg gcagcggcgc ggtgggcgct
     2101 cttggaggca tgccgcttgg accccgaccg cccggctgcg cgcggctacg agcgcacgag
     2161 cgactggcgg gtgagcacga ccgacccgga cgcggcgctg atgaaggccg ccgggcagcg
     2221 cccggccctc ggctaccatg accattacgt ggtcgacggt ggcaaggccc gcatcatcct
     2281 ccacgccctg atcacgccgg cagatgtgat ggagaacgag cccatgctcg accagttccg
     2341 gcgcatccag ttccgctggc acctccgccc caagcgcgtc gttgccgaca ccacctatgg
     2401 gacagccgag aatatcgggg ccctggagca ggcaggcatc cgcgcctacg tccccctgcc
     2461 cgactgcgag gggcgcacac cctacttcgg cagcgggcgc ttcacgtatg cgcccgcaga
     2521 cgatgcctac tgctgcccag cgggccagcc gctgcggcgg cgcaaggcga agtacaccga
     2581 gggggtcgtg gtctaccgcg ccgaggcagc cacctgcaac gcctgtgccc tgaagggcac
     2641 ctgcaccccg agcgaccacg ggcgcaccat tcaccgttcc ttccacgctg acgccctcga
     2701 gcgcgtgcgc ggttaccacg ccacggcggc ctaccagcgc gcgatgcgca agcgcaaggt
     2761 ctggccggaa ccgctcttcg ctgaggctaa gcagtggcac ggcctgcgcc agttccggct
     2821 gcgcgggctg cagaacgtca acatggaggg actgctcgtg gccgccggcc agaacctgaa
     2881 gcgctggctg agcgcgaccg gctggggccg ccgccatggc ccgaccggga gcctgagggt
     2941 tgccaggctg accattgcgt ccgtccctga gccccgctga ggcgccagtt gccgcccagg
     3001 gtctactcgc cgcagcacgt ggcgcagaca gcggtccggc ctgcccagcg cgctgcctgc
     3061 tcacgaggtc ctatttttca acagactggc ctattctgcc aaccgaacgg cccgataacg
     3121 gccattctct ccggtaccgt ctccgcagtc ctcagcctga ctcccattcc ccgagccgag
     3181 aaccgtccat cgagccaact tccccatacc caaaataagg gcagtctgca cctaatatca
     3241 g
//
LOCUS       DASUBI010000824         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_296273, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000824 DASUBI010000000
VERSION     DASUBI010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296273"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFU72_16250"
     CDS             <1..807
                     /locus_tag="VFU72_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5425095.1"
                     /translation="ATSDNLPPRFSQIQYSWRSPTLGIQQQIYNVLANNARQAAATTA
                     CQASVRWVSKTRVGLANESLSDLTYRNLALVGPPKLGEEARRFGREIQQNLDIAPMDN
                     PFEESVERLTSPQDYEANLRRSLPEWQKNFTSDDYVEYTWHAPTARLFTGRPRLKPPT
                     PGYEYPAWAYNALGGLPAAIDPGMFVAGKTIAGTLLDILTRPAELQRIQEEFKSRTGG
                     GVGGSEWVAPLLPADFAAPIELRWPEYISTVRGAHEWWIPTADGTFGEKL"
     gene            1077..>2226
                     /locus_tag="VFU72_16255"
     CDS             1077..>2226
                     /locus_tag="VFU72_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425096.1"
                     /translation="MAQPLTPEVLVYGLTNATDPQLSPDGSTMLYTLSKVDEKTHKPT
                     SQLWLCAPDGSNPRQLTQTGTRNRGGRWSPDGAAIAFVSDRVKQAGIFVMDVGQPGEA
                     RELTHHGQELNSLAWSPDGKTIAYTTTFDPENPDEEERDKDAAPPVRVTRRIDYKFDA
                     RGYLGDKRTQVWTVDVGSGERKMLTHQPVDHGNPLWSPDGKTLALSVTNANANGNHLG
                     LLDVASGEVKLIGPEGGNVTTYAWSPDGSNILYAGDTVQTWQSDFFLYDVASGETKRL
                     TDDLPVQPAGGMPGGPFARPVWLDDHEVLFSAVRAGGNGLYTFNTATRGLEPVVTLQA
                     TLSGLSLDDAQRRAVAAYSSLDGTGELFTYDLREQTSDVITDYNGELFK"
BASE COUNT          390 a          830 c          704 g          302 t
ORIGIN      
        1 gccacctccg acaacctgcc gccgcgcttc agccagattc aatactcctg gcgctcgccg
       61 acgctcggca tccagcagca gatctacaac gtgctagcga acaacgcgcg ccaggccgcc
      121 gcgacgaccg cctgccaggc gagcgtgcgc tgggtcagca agacgcgggt tggcctggcg
      181 aacgagtcgc tctccgacct cacctaccgc aacctggcgc tcgttggccc cccaaagctg
      241 ggcgaggagg cgcgccggtt cggacgcgag atccagcaga acctggatat cgcgccgatg
      301 gacaacccct tcgaggagag cgtcgagcgg ctcaccagcc cgcaggacta cgaggccaac
      361 ctgcgccgct cgctgccgga gtggcagaag aacttcacct cggatgatta cgtcgagtac
      421 acctggcacg cgccgacggc ccgcctcttc actggccgcc cgcggctcaa gccgcccacc
      481 cccgggtatg aataccccgc ctgggcctac aacgcgctcg gcggcctgcc ggccgcgatc
      541 gaccccggca tgttcgtggc cggcaagacg atcgccggca cgctgctcga tatcttgacc
      601 aggccggcgg agctgcaacg gatccaggag gagttcaagt cacgcaccgg tggcggcgtc
      661 ggcggcagcg agtgggtggc gccgctccta ccggcggatt ttgccgcgcc gatcgagctg
      721 cgctggccgg agtacatctc gaccgtgcgc ggcgcgcacg agtggtggat cccgacggcc
      781 gacggcacct tcggggagaa gctgtagagg cgtggggcgc cgccgagccg gtcgaatacg
      841 ggaatccggc gcgtcattcc ttgcggtgcc gatcccctgg cgatctccgg ctgctcgaag
      901 aacgcacgcc gtggccgccc gtccgatcct gcagctgacc ccggcgagtc ctttgccacg
      961 ggccagcaac cagccttgcc ccagtcccca cctcggttat gattcgctcg tgtgccgatt
     1021 caccgcgatg caccactcgc agggagtgga ttcgcaagca agcaaggagt aacgcgatgg
     1081 cgcagcccct gacgccggag gtgctggtct acggcctgac caacgccacc gatccgcagc
     1141 tctcgcccga cggttcaacc atgctttata cgctgagcaa agtagacgag aagacgcaca
     1201 agccaaccag ccagctctgg ctctgcgccc ccgacggctc caacccgcgg cagctcaccc
     1261 agaccggcac gcgcaaccgt ggcggacgct ggtcgcccga cggcgccgcc atcgccttcg
     1321 tctccgaccg cgtcaagcaa gccggcatct tcgtcatgga cgtcggccag ccgggcgagg
     1381 cgcgcgagct gacccaccac ggccaggagc tgaactcgct ggcctggtcg ccggacggca
     1441 agacgatcgc ctacaccacg acgttcgatc ccgagaatcc cgacgaggag gagcgcgaca
     1501 aggacgccgc gcccccggtg cgcgtcaccc gccgcatcga ctacaagttc gacgcgcgcg
     1561 gctacctggg cgacaagcgc acccaggtct ggacggtcga cgtcggctcc ggcgagcgca
     1621 agatgctgac gcaccagccg gtcgaccacg gcaatccgct ctggtcgccg gacggcaaga
     1681 cgctggcgct gagcgtcacc aacgccaatg ccaacggcaa ccacctgggg ctgctcgacg
     1741 tggcctcggg cgaggtcaag ctgatcggcc ccgagggcgg caacgtcacc acctacgcct
     1801 ggtcgccgga cggctcgaac atcctctacg ccggcgacac cgtccagacc tggcagagcg
     1861 atttcttcct ctacgacgtc gcctccggcg agacgaagcg cctgaccgac gacctgccgg
     1921 tgcagccggc cggcggcatg cccggcggcc ccttcgcgcg cccggtctgg ctcgacgacc
     1981 acgaggtgct cttcagcgcg gtgcgcgccg gcggcaacgg tctctacacc ttcaacaccg
     2041 cgaccagggg gctagagccg gtggtcacgc tgcaggccac cctctccggc ctgagcctgg
     2101 acgacgccca gcgccgcgcg gtcgccgcct actccagcct cgacggcacc ggcgagctct
     2161 tcacctacga cctgcgcgag cagacctccg acgtgatcac cgactacaac ggcgagctgt
     2221 tcaagg
//
LOCUS       DASUBI010000825         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_564962, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000825 DASUBI010000000
VERSION     DASUBI010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564962"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1125)
                     /locus_tag="VFU72_16260"
     CDS             complement(40..1125)
                     /locus_tag="VFU72_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HEU5425097.1"
                     /translation="MTFEEPLRILAIDAGAGTQDILLYESDRTPENCIKLVLPSQTQV
                     VARRIAALTARGVPLHLSGRVMGGGASSEAIERHLDAGLPVSATPAAARTLHNDLARV
                     AARGVELREDAPPGAEIIELADVDRAALAAALGRFEVALPERVAIAVQDHGYLPGAGG
                     REFRYEFLQSLLRDGGELTRMAFREPPEYMLRMRAVRELLPGAVVMDTGAAAVLGSLG
                     DPRVAEAVAGRGAVLVNIGNMHTFGIAVRGSRITGLFEHHTGGITPENLRHLVDRLRT
                     GRLTHGEVVEWGGHGAAFAADYGAGDELEFVAITGPHRRIGRALGYHEAVPHGDMMLA
                     GPFGLVEGTLNLLAREGKATGLTLVAG"
     gene            complement(1122..2072)
                     /locus_tag="VFU72_16265"
     CDS             complement(1122..2072)
                     /locus_tag="VFU72_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5425098.1"
                     /translation="MSNRSRLLALAAALVSALAALFVWEARREPRALRLRRFRVHLPG
                     LPESLAGTRIAFVTDFHVNGPGHDHGLTPLALDLLLREDPDLLLLGGDYFDRGHCHDK
                     VCHFDRLRAFALVFGVLGNHDLHRHARYTAAIVKELESRGGTILWNRAERVALRGGEI
                     TLAGVGDPYVGYADAREALQGTAEPRILLAHAPSIVDRLSPGMAALVLAGHTHGGQVR
                     FSPFDRLTPLDSTWWLDRVFRHPPQRFLRGFHWVNGSLLYVSQGVGTTRLPIRFLAPP
                     EAVLIELAARAPHPEEPCDQAARYVEELPSTTHRPDEEDR"
     gene            2239..>3376
                     /locus_tag="VFU72_16270"
     CDS             2239..>3376
                     /locus_tag="VFU72_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5425099.1"
                     /translation="MPPYRWIEPQPIAQVDELALDSPLLAAILARRGVRGASEAAAFL
                     TPELGQLGDPYDLPDLDLAVARSRAALRRGTPIAVFGDYDADGVSATAMLVRALSGLG
                     AQVRPYLPHRVRDGYGLNRGAVERIATEGAGLLIAVDCGTGNTSELALAHAHGLATIV
                     LDHHHVVNDELPGTAFVSPHREDARGFRDFCAAGVTFQFVRALVGDDEAQAYLPLAAL
                     ATVADVVPLLGANRAIVALGLARFAQAQPGLLALARQAGMRPEAVSSRDLGFALGPRV
                     NAAGRIDDPALALELLLTEDRSRALELALQLGRLNVERQRQVEGLLREAEEVLPRAEE
                     QPLIVVSHPGWNVGLVGLAASRLAERYGRPALVLAEENGVSRGSA"
BASE COUNT          469 a         1184 c         1258 g          465 t
ORIGIN      
        1 gctgtcagcg ctgctggagc gcctggccat tcgcgtcggc tagccggcga cgagcgtcaa
       61 gccggtcgcc ttcccctcac gcgccagcag gttcaaggtt ccctccacca ggccaaatgg
      121 cccggccagc atcatgtcgc cgtgcggcac cgcctcgtgg tagcccagcg cgcggccgat
      181 gcgccggtgc ggcccggtga tggccacgaa ctccagctcg tcgcccgcgc cgtagtccgc
      241 cgcgaaggcg gcgccgtggc cgccccactc gacaacctct ccgtgggtca gccggcccgt
      301 gcgcagccgg tcgaccaggt ggcgcaggtt ctccggcgtg atgccgccgg tgtggtgctc
      361 gaagagcccg gtgatgcggc tcccgcgtac cgcgatgccg aaggtgtgca tgttgccgat
      421 attgaccagc accgcgccgc gcccggccac ggcctccgcc acgcgcgggt cgcccaggct
      481 gccgagcact gccgccgcgc cggtgtccat caccaccgcg cccggcagca gctcgcgcac
      541 cgcccgcata cgcagcatgt actccggcgg ctcgcggaag gccatgcgcg tcagctcgcc
      601 gccgtcgcgc agcaggctct ggaggaactc gtaacggaac tcgcggccgc cggcgcccgg
      661 caggtagccg tgatcctgga cggcgatggc gacgcgctcg gggagcgcca cctcgaagcg
      721 gccgagcgcc gccgccagcg ccgcgcgatc gacgtccgcc agctcgatga tctcggcccc
      781 aggcggcgcg tcttcgcgga gctccacccc gcgcgccgcc acgcgcgcca gatcgttgtg
      841 cagcgtgcgc gccgcggcgg gcgtggccga gaccggcaac cccgcgtcca ggtggcgctc
      901 gatcgcctca ctgctggcgc cgccgcccat cacccgcccc gacagatgga gcggaacacc
      961 gcgcgccgtg agcgccgcga tgcgccgggc gacgacctgc gtctgcgagg gtagcaccag
     1021 cttgatgcaa ttctcggggg tgcggtcgct ctcgtagagc agaatgtcct gcgtgccggc
     1081 gccggcgtcg atagccagga tgcgcagagg ttcttcgaac gtcaccggtc ctcctcgtcg
     1141 ggtcgatgcg tggtgctcgg cagctcctcg acgtagcgcg ccgcctggtc gcagggctcc
     1201 tccgggtggg gcgcccgcgc tgccagctcg atcaggactg cctccggcgg cgccaggaag
     1261 cggatcggaa ggcgcgtggt gccgacaccc tggctgacgt agaggagact gccgttgacc
     1321 caatggaagc cgcgcaggaa gcgctgcggg ggatggcgga agacgcggtc aagccaccag
     1381 gtcgagtcga gcggcgtcag ccggtcaaag ggcgagaacc tgacctgtcc accgtgcgta
     1441 tgtccggcca gcaccagcgc cgccatgcct ggcgacagcc gatccacgat cgagggggcg
     1501 tgggccagca ggatacgcgg ctcggcggtg ccctggagcg cctcgcgcgc atcggcatag
     1561 ccaacatagg ggtcgccgac accggcgagc gtgatctcgc ctccgcgcag ggccacgcgc
     1621 tcggcgcggt tccagagtat cgtgccgccc cggctctcca gctccttgac gatggcggcg
     1681 gtgtagcgcg cgtgccggtg gaggtcgtgg ttgccgagca cgccaaagac cagcgcgaac
     1741 gcgcgtagcc ggtcgaagtg gcacaccttg tcgtggcagt ggccgcggtc gaagtagtcc
     1801 ccgccgagca agagcaggtc ggggtcctcg cgcaggagca ggtcgagcgc cagcggggtg
     1861 agcccgtggt cgtgacccgg tccgttgacg tggaagtcgg tcacgaaggc gatacgcgtc
     1921 cctgccaggc tttccggcaa gccggggaga tggacgcgga agcggcgcag gcgcagggcg
     1981 cgcggctcgc ggcgtgcttc ccagacgaag agcgccgcca gcgcactgac cagcgcggcg
     2041 gcgagcgcga gcagtcggga gcgattcgac aagcgcgatc tccgagtcca gcaccgacga
     2101 gccgtcccgg cgtcgagacc ctgccggctg cgtcctcaat cttgctgcac cgggcggggc
     2161 ggcggcaagc ggggccggcg gtgggcgaat ctggtacgat gcaggggaac gtacacgtcc
     2221 gcgtgcgaag gcggagagat gcccccctat cgctggatcg agccgcagcc aatcgcccag
     2281 gttgacgaac tggcgcttga ctcgccgctg ctggcggcca ttctggcccg gcgcggggtg
     2341 cgcggcgcct cagaggcagc ggccttcctg acgccggagc tgggccagct tggcgatccc
     2401 tacgacctgc cggatctcga cctcgccgtg gctcgcagcc gggccgcgct gcgccgcggt
     2461 acgccgatcg ccgtctttgg cgactacgac gccgatggtg tctcggcgac ggcgatgctg
     2521 gtacgcgcct tgagcggcct cggcgctcag gtgcggccct atctgcccca tcgggtacgc
     2581 gacggctacg ggctgaaccg gggagccgtg gagcggatcg cgaccgaggg cgcgggtctc
     2641 ctgatcgcgg tcgactgtgg cacgggcaac acctccgagc tggcgctggc ccacgcgcac
     2701 ggtctggcga cgatcgtgct cgaccatcac cacgtggtga acgacgaatt gcccggcacg
     2761 gctttcgtct caccgcaccg cgaggacgcg cgggggttcc gcgacttctg cgccgcgggc
     2821 gtcaccttcc aattcgtgcg ggcgctggtg ggcgacgacg aggcgcaggc gtacctgccg
     2881 ctggcggcgc tggcgacggt ggccgacgtg gtgccgctgc tgggcgccaa ccgggccatc
     2941 gtggcgctgg ggctggcgcg cttcgcccag gcgcaacctg gtctgctggc gctggcccgc
     3001 caggcgggga tgcggcccga ggcggtgagc agccgggatc tcggcttcgc gctgggaccg
     3061 cgcgtcaacg cggccggacg catcgacgac cctgccctgg cgctggagct gctgctgacc
     3121 gaggatcgca gccgggcgct cgagctggcg ctgcagctcg gcaggctgaa cgtggagcga
     3181 cagcgacagg tggaggggtt gctgcgcgag gcggaagaag tgctgcctcg tgccgaggag
     3241 cagccgttga tcgtcgtctc gcatcccggc tggaacgtcg gattggtcgg gctggcagcg
     3301 agccggctcg ccgagcgcta cggccgcccg gcgctggtgc tggccgagga gaacggggtg
     3361 agccgcggct cggcgc
//
LOCUS       DASUBI010000826         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_964890, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000826 DASUBI010000000
VERSION     DASUBI010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964890"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /gene="mutL"
                     /locus_tag="VFU72_16275"
     CDS             <1..1548
                     /gene="mutL"
                     /locus_tag="VFU72_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5425100.1"
                     /translation="SDDGSGMLPDELPLALARHATSKLRAFTDLDRLDSYGFRGEALA
                     SIAAVSELEIVSRAAGDDHATRLRAHFGRVEPPGAVPAAPGTTVTVRDLFANVPARAA
                     FLRADATEAGYAQRAVAACALARPEVRVELLLDGKPVLRSDGSGRLENAAVGVLGAEV
                     AAQLIPLAGESEDQGVRVSGLIGLPSLTRCNRQQLILLVTGRWIEHKSLAFALEQAYH
                     TLIMVGRYPVAVLNLALPPDHVDVNVHPTKREVRFREERQVFATLQRAARAALMTHTP
                     LQSVPSVAVSPLSAAGVQRRLMLADPERAGRPTPITPAPPEPEPAAVPSAAPPLRVLG
                     QVGGTYIIAEGPDGLYLIDQHAAHERVLLEELLARLARRAPEAQRLLEPLVVELNPGQ
                     HVAFEGVRAELAQLGFELEEFGGSALAIRALPAIMRRREPRETLLAILNEAALGGDGL
                     SRVEALAMTTACHSAIRAGQTLSLAEMRELVLQLEGCTAPRACAHGRPTMLHLSQTEL
                     ERQFERR"
     gene            complement(1740..>2139)
                     /locus_tag="VFU72_16280"
     CDS             complement(1740..>2139)
                     /locus_tag="VFU72_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5425101.1"
                     /translation="PSVLIQLVRRVGRGEYLINENVLQRPFVASRVLDQFRELSQVDE
                     VPGAVFSPLTPREVEILDCVAHGNSNKEIARLLNISDQTVKNHITSILRKLAVNDRTQ
                     AVIYALRHGWIKLGGEEASNGRTTDRMPVD"
BASE COUNT          302 a          770 c          748 g          319 t
ORIGIN      
        1 agcgacgatg gctcggggat gctcccggac gagctgccgc tggccctggc gcggcacgcc
       61 acctccaagc tgcgcgcgtt taccgacctc gaccgccttg actcctacgg cttccgtggc
      121 gaggcgctgg ccagcatcgc ggcggtgagc gagctggaga tcgtcagccg cgccgccggc
      181 gacgaccacg ctactcgcct gcgcgcccac ttcggccggg tcgagccacc cggcgcggtc
      241 ccggccgcgc cgggcactac cgtgaccgtg cgcgacctct ttgccaacgt accagcacgc
      301 gccgccttcc tgcgcgccga cgccaccgag gcgggctacg cgcagcgcgc cgtggcggcc
      361 tgcgccctgg cgcgtcccga ggtgcgcgtc gagctgctgc tcgatggcaa gccggttctc
      421 cgaagcgacg gctcggggcg gctggagaac gcggccgtcg gcgtgctcgg cgccgaggtc
      481 gcggcgcagc tgatcccgct cgcgggcgag agcgaggatc agggcgtgcg cgtcagcggc
      541 ctgatcgggc tgcccagcct gacgcgctgc aaccgccagc agttgatcct gctggtgact
      601 ggccgctgga tcgagcacaa gtcgctggcc ttcgcgctgg agcaggccta ccatacgctg
      661 atcatggtcg ggcgctaccc ggtggcggtg ctcaacctgg cgctgccgcc ggaccacgtc
      721 gacgtcaacg tccatcccac caagcgtgag gtgcgcttcc gcgaggagcg acaggtcttc
      781 gccacgctgc agcgggccgc ccgcgccgcg ctcatgacgc acacccccct gcaatcggtg
      841 cccagcgtgg ccgtcagccc cctgagcgcg gcgggcgtgc agcgccgcct gatgctggcc
      901 gaccccgagc gcgccgggcg tcctacgccg attactcccg cgccgcccga gccggagccc
      961 gccgccgtgc cctccgccgc gccgccgctg cgggtgctcg gacaggttgg cggcacctac
     1021 atcatcgccg aggggccgga cgggctctat ctgatcgacc agcacgccgc gcacgagcgc
     1081 gtgctgctgg aggagctttt ggcgcggctg gcgcgccggg cgcccgaggc acaacgcctc
     1141 ctggagccgc tggtagtgga gctcaatccg ggtcagcacg tcgctttcga gggcgtgcgc
     1201 gcggagctgg cacagctcgg tttcgagtta gaggagttcg gcggcagcgc gctggccatt
     1261 cgcgcgctcc cggcgatcat gcggcggcgc gagccgcgcg agacgctgct ggcgatcctg
     1321 aacgaggcgg cgctgggggg cgacgggctg tcacgcgtcg aggcgctcgc catgaccacc
     1381 gcctgtcact cggccattcg cgcggggcag acgctctcgc tggccgagat gcgcgagctg
     1441 gtgttgcagc tggagggttg caccgcgccg cgcgcctgcg cccacgggcg tcccacgatg
     1501 ctgcacctca gtcagacgga gctggagcgg cagttcgagc gccggtgagt ggccggcgaa
     1561 gacgtcgggt ggcgtgcgag gacggatccc tcgaccgctc tcgggatggc agggtgagat
     1621 aagggctgac caatacgacg ggatgtacgc gtacacccca tcccctaccc tgtcatcctg
     1681 agcctgtcga aggatccgtc ctcacccggc accgaatgac gtcgcaagcc acgcgcacgc
     1741 taatcgaccg gcatgcggtc ggtcgtgcgg ccgttcgacg cctcttcccc gccgagcttg
     1801 atccagccgt gacgcagcgc gtagatcacg gcctgagtgc ggtcgttgac cgccagcttg
     1861 cgcaggatcg aggtgatgtg gttcttcacc gtctgatcgc tgatgttcag cagccgcgcg
     1921 atctccttgt tgctgttgcc gtgcgccacg cagtcgagga tctcgacctc gcgcggcgtc
     1981 agcggcgaga agaccgcacc tggcacctca tccacctgcg acaactcgcg aaactgatcg
     2041 agcacgcgcg aggcgacgaa cgggcgctgc agcacgttct cgttgatcag atactcgccc
     2101 cgcccgaccc ggcgtacaag ctggatcagc accgacggg
//
LOCUS       DASUBI010000827         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_716224, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000827 DASUBI010000000
VERSION     DASUBI010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716224"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            580..843
                     /locus_tag="VFU72_16285"
     CDS             580..843
                     /locus_tag="VFU72_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425102.1"
                     /translation="MMGLQSRKVAWGKRDGGYPVCIFEALVPLTCADCQGTIEVGEHF
                     TRGTRGFRTTLPICAACRPVEELPAGQQLSRVSRGRMLGSSHD"
     gene            complement(876..1886)
                     /locus_tag="VFU72_16290"
     CDS             complement(876..1886)
                     /locus_tag="VFU72_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5425103.1"
                     /translation="MAEKAEPRVVVVTGASAGLGRAIVRAFARRGAHLGLLARGAAGL
                     EGARLDVEALGGKALVCATDVSDPAQVEAAAEAVERELGPIDVWVNDAMATVFSPFTE
                     MTAEDFRRVTEVTYLGFVYGTQAALKRMQPRDRGTIIQVGSALAYRGIPLQSAYCGAK
                     HAIQGFTESLRSELYHDQSKVQLTMVQMPALNTPQFTWCKSAMPRWPQPVPPIFQPEI
                     GAEAVVWAADHDRPEIYVGWPTEKAIWGNRVMPRLLDHYLARRGWYSQMTDEPDDHQR
                     PFDLYQPVDGERDFGAHGRFDSRARGFSSQLWFEQHRSWVGAAAAAAAAGLAALLVRS
                     VD"
     gene            2030..>2393
                     /locus_tag="VFU72_16295"
     CDS             2030..>2393
                     /locus_tag="VFU72_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEU5425104.1"
                     /translation="MDDVQRIVELALDEDIGLGDITSQATVPPERQARGIFLAKSDGV
                     VSGLRVAEAVFRQVDPGVDFTPSVRDGERISRGQTLATVAGTARSLLAAERVALNFLQ
                     RLSGVATATARFVAAVEGT"
BASE COUNT          371 a          837 c          831 g          354 t
ORIGIN      
        1 cgcgccgcca gtcgcggttg atcaggatgt ggtcctggtt gaccgctttg accgtcccga
       61 tctgattccc gttgctccca acgacaggca tcccctgata aatctgcgat gtcgtcacac
      121 aatgcctccc tgtctgacgc cggccccgcg ccggctcgcc acacctgccg cacatcgcgt
      181 accgcgtcac gccggcgttc ggcgtgcgct cgggctgccc actatcgcca ctacccgaag
      241 gagacagacg cggtgtcgcc acggggaggc cagcgctggc acctccgatg cggagggtag
      301 gcaggtggcg aggcgatgtc ccgtgtcaag cagcaactca cggcagcttg tgccggacac
      361 ggcgaatgcc acacgacggg gcggccccaa gggatgacgc cgcgcctgac ttcgttttgc
      421 atcgcacgcc agcgacaacg cgcctgacct gcgcagggaa aggacttaag gcacggtcca
      481 atgcgacgtc gacaggatcg caggccaggc agacgcggag accccgcgat gaattgagcg
      541 gctggaggtt tccagccggt aatcaaacga aagcgagtaa tgatgggctt gcaatcacga
      601 aaggtggcct ggggcaagcg agacggtggc tatccagtct gtatttttga ggcgttagtg
      661 ccgctcacct gcgcggactg ccagggcacg atcgaggtgg gtgagcattt cacgcgcggc
      721 acgcgcggct tccgcaccac gctgccgatc tgcgccgcct gccgcccggt ggaggagctg
      781 cccgcgggcc agcagctctc ccgcgtgtca cgcgggagaa tgctgggctc gtcgcatgac
      841 tgagggacgg cggtcgcgcc gtgcggctgg gcgcatcagt cgactgatct gacaagcagc
      901 gcggcgaggc cggcagccgc ggccgcggcc gccgcgccaa cccagctccg gtgctgctcg
      961 aaccagagct gcgagctgaa tccgcgcgca cggctatcga agcgaccgtg cgccccgaag
     1021 tcccgctcgc cgtcgactgg ctgatagagg tcgaagggcc gctggtggtc gtcgggctcg
     1081 tcggtcatct gcgagtacca gcctctgcgc gccaggtagt gatcgagcag gcgcggcatg
     1141 acgcgattgc cccagatcgc cttctcggtg ggccagccga cgtagatctc gggccggtcg
     1201 tggtcggcgg cccagacgac cgcctcggcg ccgatctccg gctggaagat cggcggcacc
     1261 ggctgcggcc agcgcggcat ggcgctcttg caccaggtga actgcggcgt gttgagcgcc
     1321 ggcatctgca ccatggtcag ttgcaccttg ctctggtcgt ggtacagctc cgagcgcagc
     1381 gattcggtga agccctggat ggcgtgcttg gcgccgcagt aggccgactg cagcgggata
     1441 ccgcggtagg ccagggccga gccgacctgg atgatcgtgc cgcggtcgcg cggctgcatg
     1501 cgcttgagcg ccgcctgggt gccgtagacg aaaccgaggt aggtcacctc ggtgacgcgg
     1561 cggaagtcct cggccgtcat ctcggtaaaa ggcgagaaga ccgtcgccat cgcgtcgttg
     1621 acccagacgt cgatcggccc tagctcgcgc tcgaccgcct cggcggccgc ctccacctgc
     1681 gccgggtcgg agacatcggt ggcgcagacc agcgccttgc cgccgagcgc ctcgacgtcg
     1741 agccgggcgc cctccagccc ggccgcgccg cgcgccagca ggccgaggtg cgcgccgcgc
     1801 cgggcgaagg cacgcacgat cgcccgcccc agccccgccg atgctccggt cactacgaca
     1861 actcgcggtt ccgctttctc cgccaccacg cactcctccc aatcgcacga acatggacag
     1921 ctcgccgcgg cgccagccgc aggcttggtg ccagtggcgc gctgccgatt gccgcgcgcc
     1981 cgcgccagcg gtaccatccg cgtggcagac ggtgggagag gagcgcgtgg tggacgatgt
     2041 gcagcggatc gtggagctgg ccctcgacga agacatcggc ctgggcgaca tcaccagcca
     2101 ggcgacggtg ccgcccgagc gccaagcgcg cggcatcttc ctggccaaga gcgacggcgt
     2161 cgtctccggc ctgcgcgtgg ccgaggccgt cttccggcag gtcgatcccg gcgtcgactt
     2221 cacccccagc gtgcgcgacg gcgagcgcat cagccgcggc cagacgctgg ccaccgtggc
     2281 cgggacggcg cgcagtctgc tggccgccga acgcgtggcg ctcaacttcc tgcagcggct
     2341 ctccggcgtg gccacggcca ccgcgcgctt tgtcgcggcg gtcgaaggca ccc
//
LOCUS       DASUBI010000828         6892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_196557, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000828 DASUBI010000000
VERSION     DASUBI010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196557"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VFU72_16300"
     CDS             complement(<1..1521)
                     /locus_tag="VFU72_16300"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5425105.1"
                     /translation="MHSLPMLSLIVYAPLVGALIIFFWATASPQVARWVAVLASAVSL
                     VFSLIVLAGFDRGASGMQYTELLHWLPGVGIDYRLGVDGISLLLVVLTTLLSLIAVLA
                     SWEPIQDRVREYMITMLLLETGMLGIFVSLDLFLFYIFWEVMLIPMALLIGIWGSANR
                     VYAAVKFFLYTLAGSLLMLVGIVATYQAYYDQTGIHTLNVLELAKGHYSNTFQILTFL
                     AFFIAFAIKVPMWPFHTWLPDAHVEAPTAGSVLLAGVLLKMGGYGMLRFNLPLFPYAT
                     EKLAPYIVALSVIAIIYGALVALVQPDLKKLVAYSSVSHMGFVTLGIFVLNSQGLYGA
                     MIVMISHGFLTSALFLCVGHIYYRGHTRLIGAFGGLATNMPVYAAFFMLFMLGSLGLP
                     GLSGFPGEFLSLLGTFRVYPVAGVIAVIVVILAAWYLMWMYQRVNWVTAPGEAMEVPH
                     APPPVMGGSGEAEAEEHEHDQGHGQGHGFVPPQTFKDLDWLEIVTLAPLAVLTLVVG"
     gene            complement(1538..3430)
                     /gene="nuoL"
                     /locus_tag="VFU72_16305"
     CDS             complement(1538..3430)
                     /gene="nuoL"
                     /locus_tag="VFU72_16305"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5425106.1"
                     /translation="MAHWLFVIPLLPLIAAVVNFLFGRWYLRHAAAWIATIAVGASFI
                     LSVICFIQVWPEGTVLNQTLYTWIPAGDFQVPAGFFVDHLTAIMLLVVTGVSFLVHVY
                     SFGYMHGDPGFYRFFSYLPLFVFSMLGLVLANNYLLLFVFWELVGLCSYLLIGFWYRR
                     RSAAQAAKKAFIVNRIGDAGFTVGIILIFIHFGTLQYDAVFASLDKVGGGMLTLIALL
                     LFAGAIGKSAQLPLFVWLPDAMEGPTPVSALIHAATMVTAGIFMVTRSAPIFAASTSA
                     MEVVAIVGAATAFVAATIGVTQFDIKKVIAYSTVSQLGYMAFALGVGAWSLAIFHLFT
                     HAFFKGLLFLGAGSVIHGMHDEQDMRFMGGLKKYLPVTFWTFLIASLANAAIVPFSGF
                     FSKDAIIESAWTSGHPVIAIVGWAASFFTALYMFRAVFMTFNGRERFDHAVVHPHESP
                     ASMKWPMILLAIPAAIAGFVGIPEHSWIGGFLDPIFAGAGVLHESSLAMTILFSVIST
                     VIALAGVGVAYAAYVSGTVSAEAWAARLGPVYRLAANRWYADEIYEYLIVHPMYVLSI
                     WLWRIVDVGIIDAIVNGVASTIAWTAQRWRRLQTGLVTNYAMIIALGMVVIVGVYLVA
                     GSTIFR"
     gene            complement(3446..3754)
                     /gene="nuoK"
                     /locus_tag="VFU72_16310"
     CDS             complement(3446..3754)
                     /gene="nuoK"
                     /locus_tag="VFU72_16310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5425107.1"
                     /translation="MSHLTADHFLFLSAALFIIGMMGVLTRRNVLIIFMSIELMTNAV
                     NISFVGFAWELHSMVGQTFALFVIAVAAAEAVVGLGIIMALARRADTVDVDAVHELRE
                     "
     gene            complement(3751..4476)
                     /locus_tag="VFU72_16315"
     CDS             complement(3751..4476)
                     /locus_tag="VFU72_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5425108.1"
                     /translation="MSVQVVVFDILGLIAVISALGVVLAKRPVHSAIALALSFINVAG
                     IYVMLRAEFLAAIQIIVYAGAILVLVLFVLMLVQEERLPEFYGGRPVQRAVGFILGLI
                     LLGEVAAAILARNISGQMGPWTTQAVQSVGGNVQALGQVLYAQNVLPIQAVALVLLVA
                     TIGALVLARPDVVEVRTGATRGIFSISLGHPRGSDDVEPETVPALEPGTTLFKSEAGD
                     RELVMARDGEEFTEQPAWGERER"
     gene            complement(4493..5515)
                     /gene="nuoH"
                     /locus_tag="VFU72_16320"
     CDS             complement(4493..5515)
                     /gene="nuoH"
                     /locus_tag="VFU72_16320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5425109.1"
                     /translation="MVPGVLTNFIVGFILLNILLGAMAYMTWFERKVLARMQDRLGPT
                     RTGPFGLLQPLADGVKLLSKEDIVPAAADRVVFLVAPVLAFILAPLGAMVIPFGGSFH
                     IGSHPVNLYVADINIAVLYVLALGSIGVYGIILGGYASGNRYSLLGGLRSAAQVVSYE
                     LVLGLSLVGIFILSGSLSLVDILNEQHRLLSFGPIQVPNWYVLSQPLAFALFMIAAVA
                     ETNRTPFDLPEAETELIGGFHTEYSGFRFSFFFLAEYINMIIVSLLAATVFLGGTAGP
                     IHDGFWWLIVKALIFLFFYVWLRATLPRFRYDQLMGLAWKVMIPLALLNIALTGLIRL
                     AGLHAL"
     gene            complement(5690..>6892)
                     /gene="nuoD"
                     /locus_tag="VFU72_16325"
     CDS             complement(5690..>6892)
                     /gene="nuoD"
                     /locus_tag="VFU72_16325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5425110.1"
                     /translation="VQISEERQLSETRREMVLNMGPQHPSTHGVLRVRLTLDGEVVTA
                     CDPIIGYLHRGVEKLGETHRYAQFTPWTDRTDYVAAPSNNLGYILTVEKLCGIEAPER
                     AQYWRMIMGELARIASHLVWLGTHALDIGALSMSLYCFREREFIMDIFERFCGARLTT
                     NMMEMGGFARPIPEGLPEMVRDFCDIFPGRQRDYEDILSANPIWLSRTRGIGIVEPAA
                     AISYGLTGACLRGSGINYDVRKAQPYLLYERIDFEVPLGTHGDVFDRYLVRMEEMRQS
                     ISIIRQCLDQVPTDGPLALTQSNYVIPQHDTVLTTAEDMQRHFIWVIKGFSPPKGEVY
                     LAIEHSKGELGHYIVSDGSPIPYRMRLRTPDFVNLQVLPYMADGAMLADVVALIGTID
                     IVLGSVDR"
BASE COUNT         1345 a         2212 c         2380 g          955 t
ORIGIN      
        1 accgacaacc agcgtcagca ccgccagcgg cgccagcgtc acgatctcca gccagtccag
       61 atccttgaag gtctgcggcg gcacgaagcc atgcccctgt ccgtgtccct ggtcgtgctc
      121 gtgctcctcc gcctcggcct cgcccgagcc gcccatgacg ggcgggggcg cgtgcggcac
      181 ctccatcgcc tcgcccggcg cggtcaccca gttgacccgc tggtacatcc acatcaggta
      241 ccaggcggcc aggatcacca cgatcaccgc gatcaccccg gccaccgggt agacgcggaa
      301 ggtgccgagc aaggacagga actcgccggg gaagccgctg aggcccggca gaccgagcga
      361 gccgagcatg aagagcatga agaaggccgc gtagaccggc atattggtcg ccagtccgcc
      421 gaaggcgccg atcagccggg tgtgcccgcg gtagtagatg tggcccacgc agaggaagag
      481 cgcgctggtc aggaagccgt ggctgatcat cacgatcatg gcgccgtaga gcccctgtga
      541 gttcagcacg aagataccga gcgtgacgaa gcccatgtgg ctgacggaag agtaggccac
      601 cagcttcttc aagtccggct gcaccagcgc caccagcgcc ccgtagatga tcgcgatcac
      661 cgagagggcg acgatgtagg gcgccagctt ctcggtggcg taggggaaga gcggcaggtt
      721 gaagcgcagc atgccgtagc cccccatctt gaggaggacg ccggccagga gcaccgagcc
      781 ggccgtcggc gcctcgacgt gggcgtccgg cagccaggtg tgaaagggcc acatcggcac
      841 cttgatcgcg aaggcgatga agaaggcgag gaaggtcagg atctgaaagg tgttgctgta
      901 gtggcccttg gccagctcca gcacgttgag cgtgtggatg ccggtctggt cgtagtaggc
      961 ctggtaggtg gccacgatgc cgaccagcat cagcaggctg ccggccagcg tgtagaggaa
     1021 gaacttgacc gccgcgtaga cccggttggc gctgccccag atgccgatta ggagcgccat
     1081 cgggatcagc atgacctccc agaagatgta gaagaggaag aggtcgaggc tgacgaagat
     1141 ccccagcatg ccggtctcca gcagcagcat ggtgatcatg tactcgcgca cgcggtcctg
     1201 gatcggctcc caggaggcca gcaccgcgat gagggagagg agcgtggtca gcaccaccag
     1261 cagcaggctg atgccgtcca cccccaggcg atagtcgatg cccacgcccg gcagccagtg
     1321 gagcagctcg gtgtactgca tgccgctggc gccgcgatcg aagccggcca gcacgatgag
     1381 cgagaagacg agcgagacgg cggaggcgag cacggccacc cagcgggcga cctgcggcga
     1441 ggcggtcgcc cagaagaaga tgatcagcgc gcccaccagc ggcgcgtaga cgatcaagct
     1501 cagcatcggg agactgtgca ccgttcgctc ctgccgccta ccggaagatc gtgcttccag
     1561 cgacgagata gacgccgacg atcacgacca ttcccagcgc gatgatcatg gcgtagttgg
     1621 tcaccaggcc ggtctgcagc cggcgccagc gctgcgcggt ccaggcgatg gtggaggcca
     1681 cgccgttgac gatggcgtcg atgatcccga catcgacgat gcgccagagc cagatcgaga
     1741 gcacgtacat ggggtggacg atcagatact catagatctc gtcggcgtac cagcgattgg
     1801 ccgccaggcg gtagaccggc cccagccgcg ccgcccaggc ctccgcgctg accgtgccgc
     1861 tgacgtaggc ggcgtaggcc acgccgacgc cggccagggc gatgacggtc gagatgacgc
     1921 tgaagaggat cgtcatcgcc aggctcgact cgtgcagcac gccggccccg gcgaagatcg
     1981 gatcgaggaa gccgccgatc cagctatgct ccgggatacc gacgaagccg gcgatggccg
     2041 ccgggatcgc cagcaggatc atcggccact tcatcgaggc gggcgactcg tgcgggtgga
     2101 cgaccgcgtg gtcgaagcgc tcccggccgt tgaaggtcat gaagacggcg cggaacatgt
     2161 agagcgcggt aaagaaggac gccgcccagc cgacgatggc gatgacgggg tggccggagg
     2221 tccaggccga ctcgatgatc gcgtccttac taaagaagcc ggagaagggc acgatcgcgg
     2281 cgttggccag ggaggcgatc aggaaggtcc agaaggtgac cggcaggtac ttcttgagcc
     2341 cgcccatgaa gcgcatgtcc tgctcgtcgt gcatgccgtg gatcacgctg ccggccccca
     2401 ggaagaggag ccccttgaag aaggcgtggg tgaagaggtg aaagatcgcc aggctccagg
     2461 cgcccacgcc gagcgcgaag gccatgtagc cgagctgact gacggtcgag taggcgatga
     2521 ccttcttgat gtcgaactga gtcacgccga tggtggccgc cacgaaggcg gtcgccgcgc
     2581 cgacgatcgc caccacctcc atagccgagg tcgaggcggc gaagatcggg gccgagcgcg
     2641 tcaccatgaa gatacccgcc gtgaccatgg tggcggcgtg gatcagcgcc gacaccggcg
     2701 tcgggccctc catggcgtcc ggcagccaga cgaagagcgg caactgcgcc gacttgccga
     2761 tggcgccggc gaagaggagc agcgcgatca gggtcagcat accgccgccg accttgtcca
     2821 gcgaggcgaa gaccgcgtcg tactgcagcg tgccgaagtg gatgaagatc aggatgatgc
     2881 cgacggtgaa gccggcgtcg ccgatgcggt tgacgatgaa ggccttcttg gcggcctgcg
     2941 ccgccgagcg ccgccggtac cagaagccga tcagcaggta ggagcagagg ccgacgagct
     3001 cccagaagac gaacaggagg aggtagttgt tcgccagcac cagcccgagc atcgagaaga
     3061 cgaagagcgg caggtacgag aagaagcggt agaagccggg gtcgccgtgc atgtagccga
     3121 aggagtagac gtgcaccagg aagctgaccc cggtgaccac cagcagcatg atcgccgtca
     3181 ggtgatcgac gaagaacccg gcgggcacct ggaagtcgcc ggccgggatc caggtataga
     3241 gcgtctggtt gagcaccgtg ccctcgggcc acacctggat gaagcagatc acgctcaaga
     3301 tgaacgaggc tcccacggcg atggtggcga tccaggcggc ggcgtggcgc aggtaccagc
     3361 gcccgaagag gaagttgacc accgccgcga tcagcggcag cagcgggatc acgaagagcc
     3421 agtgggccat gccctccctt tccctctact cgcgcagctc gtggaccgcg tcgacgtcca
     3481 cggtgtccgc ccgccgcgcc agcgccatga tgatgcccag gccgacgacc gcctccgccg
     3541 ccgccaccgc gatgacgaag agcgcgaagg tctgcccgac catcgagtgc agttcccagg
     3601 cgaagccgac gaaggagata ttgaccgcgt tggtcatcag ctcgatcgac atgaagatga
     3661 tcagcacgtt gcgccgcgtc agcaccccca tcatgccgat gatgaagagc gccgcgctga
     3721 ggaagagaaa gtggtcggcg gtcaggtggc tcatcgctcg cgctctcccc aggccggctg
     3781 ttcggtgaat tcctcgccgt cgcgcgccat caccagctcg cggtcgccgg cctccgactt
     3841 gaagagcgtc gtgcccggct ccagcgccgg gaccgtctcc ggctcgacgt cgtcgctgcc
     3901 gcgcgggtgg ccgagcgaga tcgagaagat gccgcgcgtg gcgccggtac gcacctcgac
     3961 gacgtcgggc cgcgccagca ccagcgcgcc gatggtggcg accagcagca ccagcgcgac
     4021 ggcctggatc ggcagcacgt tctgcgcgta gagcacctgg ccgagcgcct gcacattgcc
     4081 gccgaccgac tgcacggcct gcgtggtcca gggccccatc tgcccgctga tgttgcgcgc
     4141 gaggatcgcg gcggcgacct cgcccagcag gatcaggccg aggatgaagc cgacggcgcg
     4201 ctgcaccggg cgcccgccgt agaactccgg caggcgctcc tcctgcacca gcatcagcac
     4261 gaagaggacg aggaccagga tggccccggc gtagacgatg atctgaatgg cggccaggaa
     4321 ctcggcccgc agcatgacgt agatgccggc gacgttgatg aaggagaggg ccagcgcgat
     4381 cgcgctatgc accgggcgct tggccagcac cacccccagg gcggagatga ccgcgatcag
     4441 cccgaggatg tcgaagacca ccacctgcac cgacatcgcg acctctcacc tcctagagcg
     4501 cgtgtagccc cgccagccgg atcagcccgg tcagcgcgat gttgagcagc gccagcggga
     4561 tcatcacctt ccaggccagt cccatcaatt gatcgtagcg gaagcgcggc agcgtggccc
     4621 gcagccagac gtagaagaag aggaagatca gcgccttgac gatcagccac cagaagccgt
     4681 cgtggatcgg acccgcggtg ccgcccagga agacggtggc ggccaagagc gagacgatga
     4741 tcatgttgat gtactcggcc aggaagaaga acgagaagcg gaagccggag tattcggtgt
     4801 ggaaaccgcc gatcagctca gtctcggcct ccggcaggtc gaagggggtg cggttggtct
     4861 cggccacggc ggcgatcatg aagagggcga aggccagcgg ctggctgagg acgtaccagt
     4921 tgggcacctg gatcgggccg aagctgagca gccggtgctg ctcgttgagg atgtcgacca
     4981 gcgagagcga gccggagagg atgaagatac cgaccagcga cagccccagc accagctcgt
     5041 agctcaccac ctgggccgcc gagcgcagcc cgcccagcag cgagtagcgg ttgcccgagg
     5101 cgtagccgcc gaggatgatg ccgtagaccc cgatcgagcc gagcgccagc acgtagagca
     5161 ccgcgatgtt gatgtcggcc acgtagaggt tgaccgggtg gctgccgatg tggaaggagc
     5221 cgccgaaggg gatgaccatg gcgcccagcg gcgccaggat gaaggcgagg accggcgcca
     5281 ccaggaagac gacgcgatcg gccgcggccg gcacgatgtc ctctttactg aggagcttga
     5341 caccgtcggc gagcggctgc agcagcccga aggggccggt gcgcgtcgga ccgagccggt
     5401 cctgcatgcg tgccagcacc ttgcgctcga accaggtcat gtaggccatg gcgccgagca
     5461 agatgttgag caggatgaag ccgacgatga agttggtcag tacccccgga accatcgcgc
     5521 cttcccgtgc ctcgcttcgc taccccacac acccatcgac cggaacgccg tcgtccaccc
     5581 cgatcattca cccggtaggg gcaggccccc gtgcctgccc tcccccgacg cctcggtatc
     5641 agggggagca aggatggcca caaggggccg cccgtacaac catgtctccc tagcgatcga
     5701 cgctccccag cacgatgtcg atcgtcccga tcagcgccac cacgtccgcc agcatcgccc
     5761 catccgccat gtagggcaag acctggaggt tgacgaagtc gggcgtgcgc aggcgcatgc
     5821 ggtaggggat cggcgagccg tcgctgacga tatagtggcc cagctcaccc ttggagtgct
     5881 cgatcgccag gtagacctcg cccttgggcg gcgagaagcc cttgatgacc cagatgaagt
     5941 gccgctgcat gtcctcggcg gtcgtcagca ccgtgtcatg ctgcgggatg acgtagttcg
     6001 actgggtcag cgcgagcggg ccgtcggtcg gcacctgatc gaggcactgg cgaatgatgc
     6061 tgatcgactg gcgcatctct tccatgcgca ccagatagcg gtcgaagaca tcgccgtgcg
     6121 tgcccagcgg aacctcgaag tcgatccgct catagagcag gtagggctgc gccttgcgca
     6181 cgtcgtagtt gatgcccgag ccgcgcaggc aggcgccggt cagcccgtag gagatggccg
     6241 ccgccggctc gacgatgccg atgccgcgcg tgcgcgagag ccagatcggg ttggccgaga
     6301 ggatgtcctc gtaatctcgc tggcgccccg ggaagatgtc gcagaagtcg cgcaccatct
     6361 ccggcaggcc ctcggggatc gggcgcgcga agccgcccat ctccatcatg ttggtggtca
     6421 ggcgcgcgcc gcagaagcgc tcgaagatat ccatgatgaa ctcgcgctca cggaagcagt
     6481 agagcgacat cgagagcgcg ccgatatcga gcgcgtgggt gccgagccag accagatgcg
     6541 aggcgatgcg cgccagctcg cccatgatca tgcgccagta ctgagcgcgc tccggcgcct
     6601 cgatgccgca cagcttctcg accgtcagga tgtagcccag gttgttcgag ggtgcggcca
     6661 cgtagtcggt gcggtcggtc cagggggtga actgggcgta gcggtgcgtc tcccccagct
     6721 tctcgacccc ccggtgcagg tagccgatga tcggatcgca ggcggtgacc acctcgccgt
     6781 cgagggtgag ccgcacgcgc agcacgccgt gggtgctggg gtgctgcggc cccatattga
     6841 gcaccatctc gcggcgcgtc tcgctgagct ggcgctcctc gctgatctgg ac
//
LOCUS       DASUBI010000829         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1307424, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000829 DASUBI010000000
VERSION     DASUBI010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307424"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFU72_16330"
     CDS             complement(<1..894)
                     /locus_tag="VFU72_16330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5425111.1"
                     /translation="MAGEPKIRNYQVKQRLGSGTYASVWLAYDPGLERDVAVKILADN
                     WAQDDDIRRRFESEAKILWKLKDPHIVEVYGVGTLDDGRPYFEMEYADAGTLQDRIRE
                     QRDKQQFYSVAEAVRLSREIAQGLAVAHEQGVVHRDLKPSNILFKRARVVGRTTEESP
                     TRLLLADFGIARQLEQANKLTVAAGTPYYMAPEQANPTPGRGPDERCDIYAAATILYE
                     MLGGSVPYPFETVSQIVRAQERGERQGIRALNPDVPPTLAQIIDTGLEPDPEHRYRSA
                     REWKAALDEFAASLPKASDGDK"
     gene            1087..>2321
                     /locus_tag="VFU72_16335"
     CDS             1087..>2321
                     /locus_tag="VFU72_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5425112.1"
                     /translation="MAKLRIRVGDREYAFDDSQTVTIGRQADAAVPLSDALVSRRHAE
                     VRADAQGWIFRDVGSSNGTYQDGKPVREVRIAGPLDLRLGDEDGGIVVHLEPESGGET
                     QPRKPVELLAGVGTRPKPEPGPAPVPADDHPIQAFGEASFVYRPSVTMSRIGRAPEND
                     VVINDLLVSRNHAELHREGGQYVLVDLDSANGTYVNGKRITRAPIGAGDYITIGHYLF
                     RLAGGALEEYIDTGEITFEALDLTVRTDAGGVLLDDVSFSLQRCGLLAVVGPSGAGKS
                     TLLNALTGFRPADDGRVLYNDRDLYDNYDDLRQRIGYVPQDDVLHPQLTVRRALQFAA
                     ALRFPTDTAEAERDRRVEEVMGELGLAQRADLPISRLSGGQRKRASIALELLTKPSLL
                     FLDEPTSGLDPGYEKQIMTL"
BASE COUNT          351 a          816 c          792 g          362 t
ORIGIN      
        1 cttgtccccg tcgctcgcct tcggcagcga ggccgcgaac tcgtcgagcg ctgccttcca
       61 ctcgcgcgcc gagcggtagc gatgctcggg gtccggctcc aggccggtgt cgatgatctg
      121 cgccagcgtc ggcggcacgt cggggttgag cgcgcggata ccctggcgct cgccccgctc
      181 ctgggcgcgc acgatctgac tgaccgtttc gaagggatag ggcacggagc cgcccagcat
      241 ctcgtagagg atggtggcgg cggcgtagat gtcgcagcgc tcgtccggcc cgcgtccggg
      301 ggtggggtta gcctgctcgg gcgccatata gtagggcgtc ccggcggcca ccgtcagctt
      361 gttggcctgc tcgagctggc gcgcgatgcc gaagtcggcc agcagcaggc gggtcggcga
      421 ttcctcggtc gttctaccga cgacgcgcgc gcgcttgaag aggatgttgc tcggcttgag
      481 atcgcggtgg acgacgcctt gctcgtgcgc caccgccagg ccctgcgcga tctcccggct
      541 cagccgcacc gcctcggcga ccgagtagaa ctgctgcttg tcgcgctgct cgcggatacg
      601 gtcctgcagc gtgccggcgt cggcgtactc catctcgaag tagggacggc cgtcgtccag
      661 cgtgcccacg ccatagacct cgacgatgtg cgggtccttc agcttccaga ggatcttggc
      721 ctcggactcg aagcggcgcc ggatgtcgtc gtcctgggcc cagttatccg ccaggatctt
      781 gaccgcgaca tcgcgctcca ggccggggtc gtaggccagc cagacgctgg cataggtgcc
      841 gctgcccaag cgctgcttga cttggtagtt cctgatcttc ggttcgcccg ccaccgcgtc
      901 gccacccaat cacgagcaca cgactacacg agccgcgaag ccggcggaag caccgccggt
      961 ctgaccgact tgagacgaaa cagcgttgta tacgcagtat agcaagccca acgcgtgtgt
     1021 tgtaggatgt cgttatcatc accgccccag tgcgttgctc gatcgttgca cgtggttgag
     1081 gttgacatgg cgaagcttcg catccgtgtc ggcgaccggg agtatgcctt cgacgactcg
     1141 cagaccgtca cgatcggaag gcaggcggac gccgccgtgc cgctctccga cgcgctcgtc
     1201 tcgcgccgac acgccgaggt gcgcgccgac gcccagggct ggatcttccg cgatgtcggc
     1261 agcagcaacg gcacgtatca ggacggcaag ccggtgcgcg aggtgcgcat cgccggcccg
     1321 ctcgacctgc ggctgggcga cgaggacggc gggatcgtgg tccacctgga gccggagagc
     1381 ggcggcgaga cccaaccgcg caaaccggtc gagctgctgg cgggcgtggg caccaggcca
     1441 aagccggagc ccgggccggc gccggtgccg gctgacgacc acccgatcca ggccttcggc
     1501 gaggccagct tcgtctaccg cccgagtgtc accatgtcgc gtatcggccg cgcgccggag
     1561 aacgacgtgg tcatcaacga cctgctggtc tcccgcaacc acgccgagct gcaccgcgag
     1621 ggcggccagt acgtgctggt cgatctcgac agcgccaacg gcacctacgt caacggcaag
     1681 cgcatcacgc gcgcgccgat cggcgccggg gactacatca ccatcggcca ctacctcttt
     1741 cggctcgccg gcggcgcgct ggaggagtac atcgacaccg gcgagatcac cttcgaggcg
     1801 ctcgacctga ccgtgcggac cgatgcgggc ggcgtgctgc tggacgacgt ctccttctcg
     1861 ctgcagcgct gcggcttgct ggcggtggtc gggccgagcg gcgccggcaa gtcgaccttg
     1921 ctcaacgcgc tgaccggctt ccgcccggcc gacgacgggc gcgtgctcta caacgaccgc
     1981 gacctctacg acaactacga cgacctgcgc cagcgcatcg gctacgtgcc ccaggacgac
     2041 gtgctccacc cgcagctgac ggtgcggcgg gcgctgcagt tcgccgccgc gctgcgcttc
     2101 cccaccgaca ccgccgaggc cgagcgcgac cggcgcgtcg aggaggtgat gggcgagctg
     2161 ggcctggcgc agcgggccga cctgccgatc agccggctct ccggcggaca gcgcaagcgc
     2221 gccagcatcg cgctggagct gctgaccaag ccctcgctcc tcttcctgga tgagccgacc
     2281 tcgggcctcg accccggcta cgagaagcag atcatgacgc t
//
LOCUS       DASUBI010000830         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_262607, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000830 DASUBI010000000
VERSION     DASUBI010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262607"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFU72_16340"
     CDS             <1..691
                     /locus_tag="VFU72_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEU5425113.1"
                     /translation="TMAQHANVIHEDHNHWIDFRGNYELRRAALATGRKLAAAGVLGS
                     ADDTDYLTLDELRQAAANPAGRDWRALTATRAAEMERYRTLTPPPAVGTMPPGPPPDD
                     PLSRTFAKFFGVPPQPSADPDTVQGAAASAGTMIGAVKVLHSLADGGKLEPGDVMVVM
                     TTSPPWTPLFNTAGAVVSDTGGMLSHCAVVAREYGIPAVVGTGVGTRVLHDGQLVEVD
                     GTAGTVRILGA"
     gene            684..2084
                     /locus_tag="VFU72_16345"
     CDS             684..2084
                     /locus_tag="VFU72_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5425114.1"
                     /translation="MPNEAGHQRTVIGAAALSAMLAPLNSTMIVVALPALLRQFDRSL
                     AWGSWIIISYLVAMATVQPLSGSLGDRLGRRRLMLLGLIAFLAASLLAALAWNIEVLL
                     LARTAQAIAGAVAIPNGAALVRAELPVERQGSALGTVGGAVAIAAGLGPTLGGVLTDL
                     AGWRWIFALNIVLIIPALVLLLRLSDRPATGPARRFDLLGATLLTTTLVTLVLAVTLL
                     RVPSAPSLFTATLAVVGLGGGGLLLRHIARNPHPLVSPALFRAPGYSAAVFGVLLTNL
                     AMYTVLLSLPLFLAADAGWNPGATGLLLSGLSLQMVVFSPLGGRLADGRGFRLPAVLG
                     ALLILLGLLPLVAISSTWSWPMYLGALILIGIGTGLATAPIQASALLAVPAAQAGQAS
                     GFYSTMRYLGSIAGSTMMAALLAGEHPALGHVRALYALLCIFAVAGVVVCAQLPGLAR
                     EPVESDALAAGSAAPR"
BASE COUNT          246 a          794 c          743 g          342 t
ORIGIN      
        1 gacgatggcc cagcacgcca acgtcatcca cgaggaccac aaccactgga tcgacttccg
       61 cggcaactac gagctgcgcc gcgccgcgct cgccaccggc cgcaagctgg ctgcggccgg
      121 cgtactcggc tcggccgacg acaccgacta cctcacgctc gacgagctgc gccaggccgc
      181 cgcgaacccg gccgggcgtg actggcgggc gctcactgcc acccgcgcgg ccgagatgga
      241 gcgctaccgc acgctcaccc caccgccggc cgtcggcacc atgccgcccg gcccgccgcc
      301 ggacgacccg ctaagtagga cgttcgcgaa gttcttcggc gtcccaccgc aaccgtcggc
      361 cgatccggac accgtccagg gagccgctgc ctcggcgggg acgatgatcg gcgcggtcaa
      421 agtgctgcac tcgctggcgg acggcggcaa gcttgagccg ggcgacgtca tggtggtgat
      481 gaccacctcg cccccctgga caccgctctt caacaccgcc ggcgcggtgg tgtccgacac
      541 gggcggcatg ctcagtcact gtgccgtggt ggcgcgcgag tacggcatcc cggcggtggt
      601 cggcaccggc gtcgggacgc gcgtgctgca tgacggccag ttggtcgaag tagacggcac
      661 agccggcacc gtgcggatcc tcggtgccta acgaggccgg ccaccagcgc acggtcatcg
      721 gcgcggcggc gcttagcgcg atgctggcgc cgctcaactc caccatgatc gtggtggccc
      781 tgccggcgct gctccgtcaa ttcgaccgct cgctcgcctg gggctcctgg atcatcatct
      841 cctacctggt tgccatggcg accgtgcagc cgctctcggg cagcctgggg gatcggctgg
      901 gccgccggcg cctgatgctg ctggggctga tcgccttcct ggcggccagc ctgctggccg
      961 cgctggcctg gaatatcgag gttctgctgc tggcgcgcac cgcgcaggcc atcgccggcg
     1021 cggtcgccat ccccaacggg gcggcgctag tgcgtgccga gctgccggtg gagcggcaag
     1081 ggagcgcact gggcaccgtc ggcggggcgg tcgccatcgc cgccgggctg ggtccgacac
     1141 ttggcggggt gctcaccgac ctggccggct ggcgctggat cttcgccctc aatatcgtgc
     1201 tgatcatccc ggcgctggtt ctcctgctgc ggctttctga ccggccggcg accggcccgg
     1261 cgcgccgctt cgatctgctc ggcgccacct tgctgaccac cacgctggtg acgctggtgc
     1321 tggcggtcac gctgctgcgg gtgccgtcgg cgccaagcct cttcacggcc acgctggccg
     1381 tggtcggcct ggggggtggc ggcctgctgc tgcggcacat cgcacgcaac ccgcacccgc
     1441 tggtcagccc ggctctcttc cgcgcgccgg gctactcggc ggcagtcttc ggggtgctgc
     1501 tgaccaatct ggcgatgtac acggtgctgc tctcgctgcc gctcttcctg gcggcggacg
     1561 ccggctggaa ccctggcgcc accgggctgc tcctctccgg attgtcgctg cagatggtgg
     1621 tcttctcgcc gctcggcggg cgcctggccg atggccgcgg cttccggctg ccggccgtgc
     1681 tcggcgcgct gctcatcctg cttggcttgt tgcccctggt ggcgatctct tcgacgtgga
     1741 gctggccgat gtacctgggc gccctgatcc tgatcggcat cggcaccggg ctcgccaccg
     1801 cgccgattca ggccagcgcg ctactggcgg tgcccgccgc gcaggccggg caggcctccg
     1861 gcttctactc gaccatgcgc tacctcggca gcatcgccgg ctcgaccatg atggccgcgt
     1921 tgctggcggg cgagcacccc gcgctgggcc acgtgcgcgc gctctacgcc ctgctctgta
     1981 tcttcgccgt ggccggggtc gtggtctgcg cccagttgcc cggcctcgcg cgcgagccgg
     2041 tcgagtccga cgccctggcc gccggcagcg ccgcgccgcg ctagccggtc gaacgctagg
     2101 ggatgagatc gggaagcacc gccag
//
LOCUS       DASUBI010000831         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1152226, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000831 DASUBI010000000
VERSION     DASUBI010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152226"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="VFU72_16350"
     CDS             <1..2355
                     /locus_tag="VFU72_16350"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5425115.1"
                     /translation="VTTQRYTPSAGGAAVPVMEVALSQPTAEALQIKLGDFVMLESDP
                     NEPLSRFGNSPPNLAIHVVGYLQVKDPDDDYWFSDVRFDPPSVYDDGNQTQVYATALF
                     APAAYQDLLNDVSPSLLGYTYRYEVAPDRFNAGQLGTLAGDLKQLDAKYPPAFLSRPT
                     NIAVSTQLTNILKRFFAGRRFTVAILSLTGLALLAMALATIGLMASFVAVQRRATIAT
                     LRGRGASARQLLLGQASEGLLLCIPAALVGLLAADLLVHARPSVWSLVAAGATVLAAT
                     ALLVGAAAPNARRPLGTIEREEVVAARPSPRRLVFEAFIVVLAAAGVYLLRRRGLAGD
                     STAAQVGAFDPYLAAVPLLLGLALGLIVRRLFVFPVRLLAWQASFRRDLVPFLGFRRL
                     ARQSAVTSVPLLVLLLSVAVSVFSSVLLHTIEVGQKQSSWAVVGADYQVQSFLDIELP
                     KELDLSTVPGVQAAAAAYHQSDVTSGGSGGFPGTVSFLALDPDAYARVNAGRPVEPLP
                     QALFARPGADIGTANHPIPALVSRFWNGNQPLKPGDTFALHLNDQDLTFQVVQVREQF
                     AGLPLNSPFVVTQTAGVQAALAAIGPRGGTIKTNTYFVRGPASAAAPIRAALKQTAPL
                     ATLVSRSEQYASVHDSPLIAGSANGFRLGLIVAIVYSALAVGLTLALTGRARARDLAY
                     LRTLGLSWRQAIWLTVAEQAPTTVLSLILGIGLGVVVLRLISPGLDLTTFIGPGASGT
                     LVVDRWAITAIALALIVTVAAAVALVTLLVQRISLGSVLRLGEQ"
     gene            2659..3384
                     /locus_tag="VFU72_16355"
     CDS             2659..3384
                     /locus_tag="VFU72_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425116.1"
                     /translation="MTRRLSRRKVPRPHPLALQFVPGIVTVIALLPSLFDWADQSTTA
                     KILYMLGAFVLTTGAGIWQIYLPSRARNDRRKDAILNVLTSQLLQESKNYTGCECEVR
                     VNVMLARRDLNRPWRRFLEMRYAPERKYTPDEWAMRYPMGHGICGMAFERNQVMVQDL
                     SDPRVAQLVPMPPFAESLTKDVKSVMAVPIYSPDDEAKDDPIGILNLDSPEGLESSKL
                     DDPKVTLLAIKVSAFVGVVLALS"
     gene            3402..3686
                     /locus_tag="VFU72_16360"
     CDS             3402..3686
                     /locus_tag="VFU72_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425117.1"
                     /translation="MKNEELKPGLFVVKMTDKERRKKFRVDAEMIAALSGEPGSQSFA
                     QLSEDSLRAITGRSDGQPVDLLQEGRIVAGGTTTSSRTSRVPARRPGGHR"
     gene            3686..3808
                     /locus_tag="VFU72_16365"
     CDS             3686..3808
                     /locus_tag="VFU72_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425118.1"
                     /translation="MPAITAIALALIVTVAAAVALVTLLLQRISLGSVLRLGEQ"
     gene            complement(4264..>4842)
                     /locus_tag="VFU72_16370"
     CDS             complement(4264..>4842)
                     /locus_tag="VFU72_16370"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425119.1"
                     /translation="LTHLAFWAEAGSGRLPRLLRGRLAPLLGRAAPVVLARRRLIARL
                     IRVVSQLNLDYPASPLSLDCAARPRRGPRPGQRLPDATVTSDGRATRLHALLARPGIH
                     VLLSRDTPPLELPALDHDPLLAVHRLTNVPGTTVLAIRPDGYVGYRGETADPRQLTAW
                     LERAGRYPRVRRGGAAAASGNTESGRQRGCPL"
BASE COUNT          719 a         1597 c         1683 g          843 t
ORIGIN      
        1 gtgaccacgc agcgctacac cccttctgcc ggcggcgcgg ccgtgccggt gatggaggtg
       61 gcgctctcgc agccgaccgc cgaggcgctg cagatcaagc tgggcgactt cgtcatgctg
      121 gagtcggacc ccaatgagcc attgtcgcgc ttcggcaact cgccgccgaa cctggcgatc
      181 cacgtggtcg gctatctcca ggtcaaggat cccgacgacg attactggtt ctccgacgtg
      241 cgcttcgacc cgcccagcgt ctacgacgac ggcaaccaga cgcaggtcta cgccaccgcg
      301 ctcttcgccc cggccgccta tcaagacctg ttgaacgacg tcagtccgtc cctgctcggc
      361 tacacctacc gctacgaggt cgcgccggac cgcttcaacg ccggacagct cggcacgctg
      421 gccggcgatc tgaagcagct cgacgccaag tatccgcccg ccttcctcag ccgcccgacc
      481 aatatcgcgg tcagcaccca gctcaccaac atcctcaagc gcttcttcgc cgggcgccgc
      541 ttcaccgtgg ccatcctctc gctgaccggc ctggcgctgc tggcaatggc gctggccacg
      601 atcggtctga tggccagctt cgtggccgtg cagcggcgcg ccaccatcgc caccctgcgc
      661 ggccggggcg cctcggcgcg ccagctcctg ctgggacagg ccagtgaggg gttgctcctg
      721 tgcatccccg cggcgctcgt cggactgctg gcggccgatc tgctggtgca cgcgcggccg
      781 agcgtctggt cgttggtggc ggcgggcgcc accgtgctgg cagccaccgc gctgctggtc
      841 ggggcggcgg cgccgaacgc gcgccgtccg ctgggcacga tcgagcgcga ggaggtggtg
      901 gcggcgcggc cctcgccgcg ccggctggtc ttcgaggcgt tcatcgtggt gctggcggcg
      961 gcgggcgtct acctgctgcg gcggcgtggg ctggcgggcg acagtactgc cgcccaggtg
     1021 ggcgcgttcg acccctacct ggccgcggtg ccgctgctgc tggggctggc gctggggctg
     1081 atcgtgcgcc ggctcttcgt cttcccggtg cggctgctgg cctggcaggc gtccttccgg
     1141 cgcgatctgg tccccttcct cggcttccgt cgcctggcgc gccaatccgc cgtgaccagc
     1201 gtgccgctgc tggtgctgct gctctcggtc gcggtcagcg tcttctcctc ggtgctgctg
     1261 cacaccatcg aggtcgggca aaagcaaagc tcttgggcgg tcgtcggcgc ggattatcag
     1321 gtgcagtcgt tcctggacat cgagctgccg aaggagttgg acctctcgac cgtgcccggc
     1381 gtgcaggcgg cggccgcggc ctatcaccag tccgacgtta cctccggcgg ctcgggcggc
     1441 ttccctggca cggtcagctt cctggcgctc gaccctgacg cctacgcgcg ggtcaacgcc
     1501 gggcgtccgg tcgagccgct gccgcaggcg ctcttcgcca ggccgggcgc cgacatcggc
     1561 accgccaatc atccgatccc ggcgctggtg tcgcgcttct ggaacggcaa ccagccgctc
     1621 aagcccggcg acaccttcgc gctgcacctc aacgatcagg atctgacctt ccaggtcgtc
     1681 caggtacgcg agcagttcgc cgggctgccg ctcaactcgc ccttcgtggt gacccaaacc
     1741 gcgggcgtgc aggcggcgct ggcggcgatc ggcccgcgcg gcggcacgat caagaccaac
     1801 acgtacttcg tgcgtggccc ggccagcgcc gccgccccga tccgcgccgc gctgaagcag
     1861 acggcgccgc tagcgacgtt ggtctcgcgc tcggagcagt acgcctcggt ccatgactcg
     1921 ccgctgatcg ccggctcggc caacggcttc cgcctcgggc tgatcgtggc gatcgtctac
     1981 tcggcgctgg cggtcgggct gacgctggcg ctgaccggcc gcgcgcgggc gcgcgacctg
     2041 gcctacctgc gcaccttggg cctctcctgg cgccaggcga tctggctgac ggtggccgag
     2101 caggcgccga cgaccgtcct gtcgctgatc ctgggcatcg ggctgggcgt ggtcgtgctg
     2161 cgcctgatct cgccggggct cgatctgacg accttcatcg gcccgggcgc ctccggcacg
     2221 ctggtggtcg atcgctgggc gatcaccgcc atcgcgctgg ccctgatcgt caccgtggcg
     2281 gcggccgtgg cgctggtgac cctgctcgtg cagcgcatca gcctcggcag cgtgctgcgg
     2341 ctgggtgagc agtagaacaa cgcgccgggg accgtccgag ccgggacata attccggtgt
     2401 ccgtcttcag ctctcaggag gttgcttggc cagatagcgc ggtgtggtca cgatgaatgc
     2461 gttctcggaa ttcggctcga tatagcgaac gtatgcgtga actttatgga caggtcgttc
     2521 tgcgaaggag tatgatcgtt tcggtgtaaa gaagccaagc cctgttgacg aagccctggc
     2581 gcgacacgga aggtatggct cccgtgcggc tacagttagc cagcctgcat gccctggtgg
     2641 cctattggag ctgcgagggt gactaggagg ctttcccgac gcaaagttcc gcgccctcac
     2701 ccgcttgcgc ttcaattcgt gccaggtatt gtcacggtta tcgcgctgtt gccgtctctc
     2761 tttgattggg ccgaccaatc gacgactgcg aagatcctct atatgcttgg cgcctttgtc
     2821 ttgacgacgg gtgccggcat ttggcagatt tacctaccat cgcgggcgag aaacgaccgt
     2881 cgcaaagatg caattctgaa tgttttgaca tctcagctct tacaagagtc caagaattat
     2941 acgggatgtg agtgcgaggt gcgtgttaat gtgatgctag cgcggcgtga cctgaaccgc
     3001 ccatggcgcc gatttctgga aatgcgctac gccccagaaa ggaaatacac gcctgatgaa
     3061 tgggcaatgc ggtatccgat ggggcatgga atctgcggta tggcgtttga gcgcaaccag
     3121 gtgatggtgc aggatctatc tgatccccgc gtcgctcaat tggttccgat gccacctttc
     3181 gcggagtcgc tcaccaagga tgtgaaatcg gtcatggctg tgccaatcta cagtcccgac
     3241 gatgaggcta aggacgatcc tattggtata ctgaacctag atagtccgga gggattagag
     3301 tcaagcaaac tcgacgatcc caaggtcacc ttgctcgcca tcaaagtgtc ggcctttgtc
     3361 ggcgtcgtct tggcgctgag ttagttagaa caggagggca cgtgaagaac gaagaactga
     3421 agcccggtct gttcgtcgtc aaaatgactg acaaggaacg tcggaagaag ttcagggtcg
     3481 atgccgaaat gatcgccgcg ctttctggcg agcctgggag ccaatcgttc gcgcagctat
     3541 ccgaggacag cctccgtgcc attaccgggc gctcagatgg gcagccagtg gatctcctgc
     3601 aggaggggcg aatcgtggca gggggaacga ctacgagttc tcgaacgtcc cgagttcccg
     3661 cgcggcgccc tggtggtcat cgctgatgcc ggcgatcacc gccatcgcgc tggccctgat
     3721 cgtcaccgtg gcggcggccg tggcgctggt gaccctgctc ttgcagcgca tcagcctcgg
     3781 cagcgtgctg cggctgggcg agcagtaggg gcacgagcga cctcagccgg ataagaacgc
     3841 gtcgcaccgt cgttggccgc cgtggttttc ccggtagagc ggcccttctg gcgatcgaaa
     3901 ccgctcggtt ctgccggcga ggtgacgggg atcagggtga cgtgggcagc gttggtgaga
     3961 gccggcaata gggggaagag ggcaggcacg ggggcctgcc ctaccggtgt cgatgccagg
     4021 cgtaccctcg gcacaccggc aacaggggac acgaggcaac gtcgggcgta tctcggtggg
     4081 ggcgcgttcc tccaacgcac cggcaacgag ggaacgcccg gcgttgcccg ggggtagggg
     4141 cggtccccgt ggccgccctc tcgcgcgcac gcatcggcaa gggaggcgta gggcaacggt
     4201 gcgcggcgga cgtgatggcg ggtgccgggg tcacgagggc agaggtgggc gcgctgggct
     4261 gccctacagc ggacaacctc tttgtctacc gctctcggtg ttcccgctcg ccgcggccgc
     4321 cccccctcgt cgcactcgcg gataccggcc ggcgcgctcc agccaggcgg tgagctgccg
     4381 cggatcggcc gtctccccgc gatagccgac gtagccgtcc ggccggatgg cgagcaccgt
     4441 ggtgccgggc acgttagtca gccggtggac ggcgagcagg ggatcgtggt cgagagctgg
     4501 cagctccagc ggcggcgtgt cccgcgatag cagcacgtgg atgcccggcc gcgccagcag
     4561 ggcgtgcagg cgggtggcgc gcccgtcgct ggtgacggtg gcgtccggca ggcgttggcc
     4621 cgggcgcgga ccccggcgcg ggcgtgcagc acagtccagc gacagcgggc tggcggggta
     4681 gtccaggttc aactgcgaga cgacgcggat caggcgcgcg atcagccggc gccgggcgag
     4741 gacgaccggc gccgcccggc cgaggagcgg ggcgagccgg ccgcgcagca gccggggcag
     4801 gcgcccgctg cccgcctccg cccagaaagc caggtgggtg ag
//
LOCUS       DASUBI010000832         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_108757, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000832 DASUBI010000000
VERSION     DASUBI010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108757"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFU72_16375"
     CDS             complement(<1..291)
                     /locus_tag="VFU72_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425120.1"
                     /translation="MAQSENQSRKVGLIVGREWSFPPAFIEEVNKRDEGVEAEYVKLG
                     GTRMEEPIPYSLIIDRISHEVPYYRTYLKYAVAQGVYVVNNPFMWTADDKFFE"
     gene            complement(333..1058)
                     /locus_tag="VFU72_16380"
     CDS             complement(333..1058)
                     /locus_tag="VFU72_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5425121.1"
                     /translation="MGISRQYHRWFSPALHRDMELLVFGHGGARVIVFPTSMGRFYEW
                     EDRGMVAALGDQLANGWIQLYCVDTVDGESWYNRHKPGWAQAMRQAEYERYLLGEVVP
                     LMDNLNGTPYTMTTGASLGAYHAANFAFKNPWLINRLIGMSGIYGIDRFVNGHFDENV
                     YFNNPPAYLQHEHDPARIGALQRQDIIIVTGTDDPNRASSERLSTVLWDKGIGNALRL
                     WHGWCHDWPYWRDMIRTYIGGHD"
     gene            complement(1848..2006)
                     /locus_tag="VFU72_16385"
     CDS             complement(1848..2006)
                     /locus_tag="VFU72_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425122.1"
                     /translation="MMTFLWVVLGFSLVGLLLAVTARLFGRGMWGAEYSESHYLDEVE
                     AQNRIPGP"
     gene            2194..>3275
                     /locus_tag="VFU72_16390"
     CDS             2194..>3275
                     /locus_tag="VFU72_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU5425123.1"
                     /translation="MRIAIGGIAHETNTFCTGLTTMEAFTERESGTGAEITARHQGVR
                     SYIGGMLDAAADLGIKVVPTFYASATPSGTISRATFDSLLERLLSGIRAAMPVDAVAL
                     ALHGAGVAEGADDIESVILTRVRELVGEDTPIVVTLDLHGNMTQEMLAHADALFGVHL
                     YPHTDAYERGREAVELIPRLLSGEVKPAMHLTMLPMMIPTSTTNLPPASEVNARCWEW
                     ERREGVLDCAFFHGFPTTDIPRPTVSVLAMTNNDPALAREIANDVAGFIWEIQERFRP
                     VITPPEEAIRQALAAEGRPVVINDTADNPGGGAPGDATHLLRAMLEANLQDACFGYIT
                     DSETAAQAHAAGVGATIQVRLGGKTEP"
BASE COUNT          573 a         1081 c         1078 g          543 t
ORIGIN      
        1 ctcgaagaat ttgtcgtcgg cggtccacat gaaggggttg ttgaccacgt agacgccctg
       61 cgccacggcg tacttgaggt aggtgcggta gtagggcacc tcgtgcgaga tgcggtcgat
      121 gatcaggctg taggggatag gttcctccat gcgcgtgccg ccgagcttga cgtactcggc
      181 ctccaccccc tcgtcgcgct tgttgacctc ctcgatgaag gccggcggaa acgaccactc
      241 gcgcccgacg atcaagccga ctttgcgtga ttggttctcg ctctgggcca tgccgctcct
      301 ccgctcgctc tctgcttcct gatgcacgcg gctcagtcgt gcccgccaat atacgtccga
      361 atcatgtctc tccagtaggg ccagtcgtga caccagccat gccagagccg gagcgcgttg
      421 ccgatcccct tgtcccagag gacggtcgag aggcgctcgc tgctggcgcg gttggggtcg
      481 tcggtgccgg tgacgatgat gatgtcctgt cgctgcaggg ccccgatgcg cgccgggtcg
      541 tgctcatgct ggaggtaggc gggcgggttg ttgaagtaga cgttctcgtc gaagtggccg
      601 ttgacgaagc ggtcgatgcc gtagatgccg ctcatgccga tcagccggtt gatcagccag
      661 gggttcttga aggcgaagtt ggccgcgtgg taggcgccga ggctggcgcc ggtggtcatg
      721 gtgtagggcg tgccattcag gttatccatc agcggcacga cctcgccgag caggtagcgc
      781 tcgtactccg cctgacgcat ggcctgggcc caaccgggct tgtgccggtt ataccagctc
      841 tcgccgtcga ccgtatcgac gcagtagagc tggatccagc cgttggcgag ctggtcgccg
      901 agcgcggcca ccatgccgcg gtcctcccac tcgtagaagc ggcccatcga ggtggggaag
      961 acgatcacgc gcgccccgcc gtggccgaag accagcagct ccatgtcgcg atggagcgcg
     1021 ggagagaacc agcggtgata ctgccggctg atgcccatga ccctccaacc cccgccgcgc
     1081 ggcgcatcgt ccagcggcgg cccctccccc gcccgctgca accatcatgc caatggcggc
     1141 tcatggtacg gggtgggcac agggtagcat cgaatgggca gcagggatag ttggaccggt
     1201 tggcgtgttg cgtcaaggtt tggtggcgtg aggcgcgcgg ggtgagcgga cggccgccat
     1261 gatccacagg cccttgtcgt gcacggcgcg ctgggctgaa gccgcagcgc gagatctttc
     1321 cgtaggcctg ctgatggagt ttggcgaatt aatccgggtc atgggtgccg cccgacgccc
     1381 gggaatcgaa ttccggacgg agccgggctg aatccacgaa gcccactgaa ggggctgggt
     1441 tgggacacgg ggctgtgcca cactacttcg tcacagtcca tcagcaggct aagattgaag
     1501 gaatctctca gcctgctttc agcaggctta gccttctagc gcggcggctt tagcgcgtgt
     1561 ctgacgactc cattgcgtgc caggtgcggc acggatccct ccactccggt cgggatgaca
     1621 ggggagggaa tgcagcctgc cgggcgagat aagccgtcgc ccaagactca ccgtcctcgg
     1681 cagcccgaca ctacccctgt catcctgagc gcagtcgaag gatccgtgcc ccacgcggca
     1741 cgcaatggag tcgtcagaca gactctagcg ccgcgcggtc ccgcgtcgga gacggagtga
     1801 attcgtcaaa gactaataac cctgcgcgaa caaggaaccc gccgtgtcta cggaccagga
     1861 atccggttct gcgcctcgac ctcgtcgaga tagtgcgact cggaatactc cgcgccccac
     1921 atgccccgac cgaagaggcg cgcggtgacc gcgagcagca acccgaccag cgagaacccg
     1981 agaacgaccc agagaaacgt catcattatg cgcctccacc ttggattatc gcatccaaaa
     2041 gtccacgatg caatcgtgta attcatgagg aagctgaatc gcgtgcggat cgacggccat
     2101 ggcgtgacgc cggtgtgctt gctcgcgccg ccatccccgg ctaccatgcg cgcacggcac
     2161 aggggacgat tcacgaacga gagggagaga gacgtgcgca tcgccatcgg gggcatcgcc
     2221 cacgagacga atacgttttg cactgggctg acgaccatgg aggccttcac cgagcgcgag
     2281 tcggggaccg gcgccgagat cacggcgcgg caccagggcg tgcgtagcta catcggtggc
     2341 atgctcgacg ctgctgccga tctcggcatc aaggtcgtgc caaccttcta cgccagcgcc
     2401 acaccatcgg gcacgatcag ccgcgccacc ttcgactccc tgcttgagcg cctcctgagc
     2461 ggcattcgcg ccgccatgcc ggtggacgcc gtcgcactgg cactgcacgg cgccggggtg
     2521 gccgaggggg ccgacgacat cgagagcgtg atcctgacaa gagtgcgtga gctggttggc
     2581 gaggacacgc cgatcgtggt cacgctcgac ctccacggca atatgaccca ggagatgctg
     2641 gcgcacgccg acgccctctt cggcgtccac ctctacccgc acaccgacgc ctacgagcgc
     2701 gggcgcgagg cggtcgagct gatcccgcgc ctcctctccg gcgaggtcaa gccggcgatg
     2761 catctgacga tgctgccgat gatgatcccc accagcacga cgaatttgcc gcccgcctcc
     2821 gaggtcaacg cgcgctgctg ggagtgggag cggcgggagg gcgtgctcga ctgcgccttc
     2881 ttccacggct tccccaccac cgacatcccc cgccccaccg tcagcgtgct ggccatgacc
     2941 aacaatgacc cggcgctggc ccgcgagatc gccaacgacg tggccggctt catctgggag
     3001 attcaggagc gctttcgccc ggtcatcacg ccgccggagg aggcgatccg ccaggcgctg
     3061 gccgccgagg gtcgcccggt ggtgatcaac gacaccgccg acaaccccgg cggaggcgcg
     3121 cccggcgacg ccacccacct gctgcgcgcc atgctggagg cgaatcttca ggacgcctgc
     3181 ttcggctaca tcaccgactc cgagacggcg gcgcaggcgc acgcggcggg tgtcggggcc
     3241 acgatccagg tgcgcctcgg cggcaagacc gaacc
//
LOCUS       DASUBI010000833         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_48919, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000833 DASUBI010000000
VERSION     DASUBI010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48919"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFU72_16395"
     CDS             complement(<1..251)
                     /locus_tag="VFU72_16395"
                     /inference="COORDINATES: protein motif:HMM:NF020858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425124.1"
                     /translation="MTDLPYGVARPPGGEPRCVTRVEDRAIDLSALDLPVPPETFSAP
                     SLNPFLALGSSAWAEVAGRLAELADPSLPGWPLGEVELLL"
     gene            complement(248..1051)
                     /locus_tag="VFU72_16400"
     CDS             complement(248..1051)
                     /locus_tag="VFU72_16400"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU5425125.1"
                     /translation="MSARVSVSVANHVAVVELSRPDKHNALDGAMFEGIVEAAEEVAR
                     DASVRAVVLHGAGPSFCSGLDLGSLMTGPVSLDDILQRPDGERANAAQRTCTAWIDVP
                     APVIAAIHGNCFGGGLQIALGADIRFAAPDARLSVMESKWGLVPDMGITSTLPRLVRI
                     DVAKELTYTGRVVTGTEAAALGLVTRAVEDPLAAAKELAAEIAGRSPDAMRAAKKLYD
                     TSWHGPVEEGLLLETELQVGLIGSPNQIEAVRSGLAKEPADFADPASVA"
     gene            1199..1777
                     /locus_tag="VFU72_16405"
     CDS             1199..1777
                     /locus_tag="VFU72_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU5425126.1"
                     /translation="MATQLSGSSFVKTDLTVDVSAAPAPVVATAARDLCEGHRHPAVM
                     CGLGNGRRTRGGGEKASHIGWPAVTGILWIVRESTTGQSDTGTDFNDELLGSHGNDSV
                     SGGAGSDILWGDQLPVGNNSWQHDTLDGGAGNDWIYSSHGKNTIRGGAGNDHIWGHFG
                     HGTIDCGSGWDVVHVKRHPTYRLRNCERVLHH"
     gene            complement(1766..>2174)
                     /locus_tag="VFU72_16410"
     CDS             complement(1766..>2174)
                     /locus_tag="VFU72_16410"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEU5425127.1"
                     /translation="LLRGRLLGMARGWGQGVGPVAVLACLPGEQHDLGLISFGLALRE
                     RGWRIVYLGPDAPIETVMQIGAQPEPVAVVLSAVSEERVEPVLPALRELAAQRRVALG
                     GGAAQAASGELNDLLRLDGDLVSEAARLTSLVV"
BASE COUNT          345 a          789 c          761 g          279 t
ORIGIN      
        1 agcagcagct cgacctcgcc gagcggccag ccgggtaacg agggatccgc gagctcagcc
       61 aggcggccgg ccacctccgc ccacgcactg gagccgagcg cgagaaacgg gttgagtgag
      121 ggcgccgaga acgtctccgg aggcaccggc aggtccagcg cgctcaggtc gatcgcgcgg
      181 tcctcgaccc gcgtgacgca gcgcggttcg ccacccggcg gccgcgcgac gccgtagggc
      241 aggtcggtca cgcgaccgac gcagggtcgg cgaagtccgc cggctccttg gccaggcccg
      301 agcgcacggc ctcgatctgg ttgggcgagc cgatcagccc gacctgcagc tcggtctcca
      361 gcagcagccc ctcctcgacg ggcccgtgcc agctggtgtc gtagagcttc ttggccgcac
      421 gcatcgcgtc gggcgagcgg ccggcgatct ccgcggccag ctccttcgcg gcggccagcg
      481 ggtcttcgac cgcgcgcgtg accaggccga gcgccgcggc ctcggtgccg gtgacgacgc
      541 gcccggtgta ggtcagctcc ttggcgacgt cgatgcgcac cagccgcggc agcgtcgagg
      601 tgatgcccat gtcgggcacc aggccccact tggactccat caccgacagc cgcgcgtcgg
      661 gcgccgcgaa gcggatgtcg gcgcccagcg cgatctgcag gccgccgccg aagcagttgc
      721 cgtggatcgc cgcgatcacc ggcgccggca cgtcgatcca ggcggtgcag gtgcgctggg
      781 cggcattggc ccgctcgccg tcgggccgct gcaggatgtc gtccagggat acaggcccgg
      841 tcatcaacga gccgagatcg agacctgagc agaagctcgg ccccgcgccg tggagcacga
      901 ccgcgcgaac cgacgcgtcg cgcgcgacct cctcggcggc ctcgacgatg ccctcgaaca
      961 tcgcgccgtc gagcgcgttg tgcttgtccg ggcgggacaa ctccacaacg gcgacgtggt
     1021 tggccacaga gacggaaacg cgcgcgctca tggcccaacg ctacgacaag cgtccaggaa
     1081 tgcgtcgaca acctaagtag cggggcatga cgccgatggg ggaagtatgc gtcgattcgt
     1141 ccactgcctt tgggttgtgg ctgctgttct tgctgcgccc acggcggctc acgctgccgt
     1201 ggccacgcag ctcagcggca gctccttcgt caagaccgat ctcacggtcg acgtcagtgc
     1261 agcgcccgca ccggtcgtgg ccaccgcggc tcgcgacctc tgcgaaggcc accgccatcc
     1321 ggccgtcatg tgcggtcttg gcaacggccg gcgcacgcgc ggcggcggcg agaaggccag
     1381 ccacatcggt tggccggcgg tcaccgggat cctctggatc gtgcgcgaaa gcacgaccgg
     1441 ccagagcgac accggcacgg acttcaacga cgagctgctc ggctcgcacg gcaacgactc
     1501 cgtcagcggt ggcgctggga gcgacatcct ctggggtgac cagcttcccg tcggcaacaa
     1561 cagctggcag cacgacacac ttgacggcgg cgccggcaac gactggatct actcgagcca
     1621 cggcaagaac acgatccgcg gcggcgccgg caacgatcac atctggggcc acttcggcca
     1681 cggcacgatc gactgcggca gcggctggga tgtcgtgcac gtcaagcgcc acccgacgta
     1741 ccggctgcgc aactgcgagc gcgtcctaca ccactagcga ggtgaggcgg gcggcctcgg
     1801 agacgaggtc gccgtccagt cggagcaggt cgttgagctc gccgctggca gcctgcgccg
     1861 ccccacctcc gagcgccacc cgccgctggg cggccagttc gcggagcgcc ggcagcaccg
     1921 gctcgacccg ctcttcgctg acagcgctga gcacgaccgc gacaggttcc ggctgggcgc
     1981 cgatctgcat cacggtctcg atcggcgcgt cgggtccgag gtagacgatg cgccagccgc
     2041 gctcgcgcag cgccaggccg aaggagatca gaccgagatc gtgctgctcg cccgggaggc
     2101 aggcgagcac cgcgacgggt ccgacaccct gaccccagcc gcgtgccatc ccgagcagcc
     2161 ggccacgcag cagg
//
LOCUS       DASUBI010000834        13598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1302197, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000834 DASUBI010000000
VERSION     DASUBI010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302197"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFU72_16415"
     CDS             complement(<1..544)
                     /locus_tag="VFU72_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425128.1"
                     /translation="MPTPAGGPRRYSPELAVSLRIASDVQLAPDGARVAWVVAPVGHE
                     ETRPTSTIWLAEVGPEPAPRPFTGGKAEDRMPRWAPDGGRLAFLSDRATRGSAQIYLI
                     DRQGGEAQPLGALPGAADLLAWLPDGSALSCTITRRALAGEQESSSEIKVASQSDRPQ
                     TIVRVPLDGGAPAAIGPAEGH"
     gene            complement(630..1394)
                     /locus_tag="VFU72_16420"
     CDS             complement(630..1394)
                     /locus_tag="VFU72_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425129.1"
                     /translation="MSGEQEREGPSDDEELFEEIRRFLGEQSGEDIYEDFRAAFQGAR
                     GFVNRELRQMEEYGRMIRTLERLLEDEFPSTFNPALRTRLAEILYAYGLAHYSLLQHQ
                     AVEDAIDEADEEDEEDVVRPLVIYDLWFPQDIAIESAQDGYYLHLSDGRIDLSLYLGA
                     EPNERGALVDLVDHWGYAATNTPPGEPPQPGERITPVLTVELDYGGSELVDADDHVDI
                     LFLDHNGEPIVRMHARAAELRAIVDELQNIGRDQEL"
     gene            complement(2064..3458)
                     /locus_tag="VFU72_16425"
     CDS             complement(2064..3458)
                     /locus_tag="VFU72_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5425130.1"
                     /translation="MGDTATTAKDLTKSEALERQALEHVWIQTTEWIRLAEQDGLHVF
                     ERAHGSTLVDVRGREWIDGISGLWVVNAGHGRAEIGEAMAEQAAKIAYASSASYTTVP
                     AVQLANVLSQITPGDLNRVYFCSGGSEAVESAIKIAKQIQVMRGFPKRYKIIARRGSY
                     HGMTHGAMSLTASRNETYFGPFMYGVYHTPSPNHYRNDFGLEGEAGDVMCANYVEQEI
                     INQGPETVAAVIGEPISTSNGVHIPSPKYWQRLREICDKHGVLLIMDEVINGFGRSGK
                     MFATEHFGMQPDLMTMAKGLSSGYAPIAACAVSDRVFEIFREKKETALGHLLTFGGQA
                     VACAAALKNLEIMQAEHLPEQSAEKGRYLLDKLEGLRTHPTVGDVRGLGLMCGVEIVQ
                     NKDTKAKWSKESGFVKALDKRINDKGMLTRTWDIVHVAPPLVVTYPELDRIVTIIDES
                     LTETEAEFADQVGK"
     gene            3751..5652
                     /locus_tag="VFU72_16430"
     CDS             3751..5652
                     /locus_tag="VFU72_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5425131.1"
                     /translation="MLDHTSSPRRADEPRGRLTFSRRHVLFASGASALAALLAACGGS
                     SAPSTPAATANLNAPAPTSAPNLGLASPAATSAATSAATSATTAAASPAAGGAEIGKL
                     VLNTEPYPKYTGTPKPGDTLTIIRNEDLSDLNPAALNSYSPYTFSYDPLVWIDEYTLD
                     PKPWLATSWEVTPDGKSYTYKLRNDIKWQDGTPFTADDVAFSMVVYRDDPESGVARFF
                     PLMKQDPTVVDAQTIRFDLSDPSGDWVLNASNQFMLQKKQFGDYWNSGKGEKGAKTLK
                     GFPYTEQMLIGTGQWKQTGYAPGSAPPYLQYEANPNYFQNAPHFQKLIFKEVDEAQAR
                     ITAWLNNETDLLWPITATDVDQVKGQDGWLYSAYAVAFMCAWINFKNPKVVNAGFLND
                     KAVRQALSIGIDRAGFAQSVFHGFVDQLKIGSVAFPWAYNTTLKSPDHDAAKAKQMLE
                     AAGYKTGSDGKLQDKSGNPVKLVAITANNNQYPVDKIAVSVQEDFRQLGIDMQIDTLE
                     PAALKARWQKTFDWDLYFYSRILFAGFSDYNYYHSAWDPRTNPQGRDFGAWKNTDADK
                     LLDQIIREPDLKKQKDLLWQFQAVIADDMPAFWFGFPHDLILVKKNLQGYQPNAMWQY
                     WDTWKLWRT"
     gene            5727..6674
                     /locus_tag="VFU72_16435"
     CDS             5727..6674
                     /locus_tag="VFU72_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5425132.1"
                     /translation="MTRFVVRRLVQAIPLLIGISLVVFLMIYLSPGDASTFALSERAN
                     EAQVQAYRHYMGLDLPWYRQYLRLGGNWLRGDLGTSLIQRRPVSTIIAERLPRTVELV
                     GIATILALLVAIPIGVFSAIRQYSVSDHVVTVASFVGIAIPSFWLGIMLILLFSVKLH
                     WLPTGGTQTIGQPWSLVDHLKHLILPAAVLTIIQTAAWSRYMRSSMLEVLVQDYMRTA
                     RAKGLPERLVLLRHAMRNAMLPIITLLGLSLPTLVGGAIITEQIFSWNGLGKLVVDSA
                     SKRDTPVVLALVMLSAIAIVVGNLLADIGYRLIDPRIEL"
     gene            6686..7603
                     /locus_tag="VFU72_16440"
     CDS             6686..7603
                     /locus_tag="VFU72_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5425133.1"
                     /translation="MDEQAVIAHPEPLGTHRLDLELRQGAQTSVLRRFGQNPLALVAL
                     LVLALLTLSALTAPLWLPRDRVLDQVRSATFASPSLHALFGRDEYGRDAFARVVYGGR
                     VSLWVSFSSAIIATLLGTVLGMIAGYLGGWADSVIDRLIELVLTIPFLLLLILAASMA
                     RPSQTSIILILGLFGWPVLARLVRGEFIALRRRDYVDAARTAGCSSTRIILRHILPNA
                     MAPIIVTGTLILATNLTAEAAISFLGLGIQPPTPSWGNLLTNAQNYVISAPFLAIIPG
                     LMILITVLCVNVLGDALRDALDPRLRGRH"
     gene            7702..8613
                     /locus_tag="VFU72_16445"
     CDS             7702..8613
                     /locus_tag="VFU72_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5425134.1"
                     /translation="MATQAVDERIRALIQEAGVGGTVGVAAHKLGTSEQIMLNPDTVF
                     PTASSFKVVLLYTLYRLADAGTVDLGARVALEERHRVPGSGVLQDLDAGAVLTVKDIA
                     TLMIVMSDNSATDMTYDLVGRDAVQQSLDRLDMPHTHLPLGCWGILAGLRDLDPNDPD
                     LTYAELKRRLGEEEGSWDCAALAETPENNVSTPRDMLRLMEGINAGEGLSAGSRAAVI
                     DILLRQKISDRIPALLPFGVRAAHKTGSVKGVRNDVGLIFAGETTYALALMSKGAQDE
                     YEVTQLLARISRAIYDAFAGAPATDPA"
     gene            8647..9885
                     /locus_tag="VFU72_16450"
     CDS             8647..9885
                     /locus_tag="VFU72_16450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5425135.1"
                     /translation="MLDRSGGARGGELSQRDLVPFYLGVLSGPMGSGPVIVLFVVLMD
                     AFGVGRGALSLAVTAYMIPFATVQLISGGISDLTSRRSSLLGGFGAFGAATIFAGLAP
                     SFGLFLLAQALQGAANAFTTPLVLATLGDITHGRQLSRAVGLFSTANIAGFLAAPLVA
                     GFLGGISWRAPYLAVGLYTWAFAAWYWFWFRRHGASVPPRPRSGSLGADSRRILRALG
                     LTVALLAAISFCYSVATRGASYLFADYLQHRWGSSVEAAGVILATFGLGGLLLGPLSG
                     DVITRIGLYRGAALSMLGVALSLILLALAPSPLAFAGANLLYGVAAVYAWTALNMLVV
                     EAVPAHRGTVSSIFGAARFYAMGVAPLWFTPLYQSLGARSIYPVSAVFALALLPPLLR
                     LRGHHARVIPGATADDLARR"
     gene            10098..10253
                     /locus_tag="VFU72_16455"
     CDS             10098..10253
                     /locus_tag="VFU72_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425136.1"
                     /translation="MSAYRTQYPLRPDTLPRALLLEMFGIEYFVQARPCRAVESEILR
                     VPAAIER"
     gene            10458..10721
                     /locus_tag="VFU72_16460"
     CDS             10458..10721
                     /locus_tag="VFU72_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425137.1"
                     /translation="MAPTGKPQRLYLLQLGTTTIPGARGPLSMSSGCYLVQTSADEHI
                     LIDSGLPSDYTPPAGAPAAENQRDVLQHLATLGLAPGDIDLVI"
     gene            10734..11192
                     /locus_tag="VFU72_16465"
     CDS             10734..11192
                     /locus_tag="VFU72_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5425138.1"
                     /translation="MDHVGYHRSFPSAAFVVQREHYELARAGHARFAAATSYWDDPRL
                     HYRMLDGDTELLPGLTLLKTPGHAPAHQSVLVRLPQTGSVLLAIDAVMFARQFTADRA
                     AWPMDDNEAELRASTRKLLDLVEREQVALVVFGHDGEQWQALKKAPEYYA"
     gene            complement(11244..12917)
                     /locus_tag="VFU72_16470"
     CDS             complement(11244..12917)
                     /locus_tag="VFU72_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425139.1"
                     /translation="MASYDDDYDYDLGPHGRTITTSSPEAQRWFDRGLNWTYGFNREE
                     AQRCFERALEHDPACAMAHWGVAYAAGPYYNLRWDMLTDPARQAMVATCHAASQRALA
                     LIEGANPADQALITALTARYPQAVPVEDCRVWNDDYADAMRAVYHDFADDLDVAAFFA
                     EALLNRTPWQLWDLPTGQPAEGASTLEARAVLERALADPASQRHPGVLHQYIHLMEMS
                     PEPEQALRAADWLRDLVPDSGHLRHMPTHIDTLCGSYYSAVTCNTAAIAADNKYAARQ
                     GTMNMYTFSRLHNHIVRLYAAMFLAQSEIALESADAILDIIPRQLLEQQTPIVMADRL
                     EGFIPAKLHVLIRFGRWQEIIDTPLPDDPELLCYTTAMTHYAKGIAYAVTGRIPAAEE
                     QQRLFVAAAARVPETRWHQGNRCVDLLLIAGAMLAGELEYRKGNYDDAFAHLRESVRL
                     YDELLYSEPWGWMQPARHALGALLLEQDRVEEAEAVYKADLGLGGSLARAHQHPGNVW
                     SLHGYHECVMRLGKHDLCGPVSQRLTLASARADVPIDASCFCRLTHAAD"
     gene            13109..>13598
                     /locus_tag="VFU72_16475"
     CDS             13109..>13598
                     /locus_tag="VFU72_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425140.1"
                     /translation="MIETVPATLHDFFVASSGASAALIGLLFVAVSVAPERVFGPGAS
                     GERTYRAGSAFFVLSNGLFISLGALLPQLNLGDILVLVGAGGLLNILLLIIELWQRGA
                     RPAGHGLLLALGSVALYAGEVRFGIGLLRHPNDQRAIDAVTYMLLAGYVIGLARAWQL
                     VSG"
BASE COUNT         2001 a         4789 c         4543 g         2265 t
ORIGIN      
        1 cgtgcccctc ggccgggccg atggcggccg gggcgccgcc gtcgagcggc acacgcacga
       61 tcgtctgcgg ccggtcggac tggctggcga ccttgatctc gctgctcgac tcctgctcgc
      121 cggccagcgc gcgacgggtg atggtgcagc tcagcgcgct gccgtcgggg agccaggcga
      181 gcaggtcggc cgcgccgggg agcgcaccga gcggctgcgc ctcgccgccc tggcggtcga
      241 tcaggtagat ctgggcgctg ccgcgggtgg cacgatcgga gaggaaggcc aggcggccgc
      301 cgtcgggcgc ccagcgcggc atgcggtcct ctgccttgcc gccggtgaag gggcgcggcg
      361 ccggctcggg gccgacctcg gccagccaga tggtcgacgt cgggcgtgtc tcctcgtgcc
      421 cgaccggggc gacgacccag gccacccggg cgccatccgg cgcgagctgc acgtcgctgg
      481 cgatgcgcag gctgaccgcc agctccgggc tgtagcgacg tggtccccca gcaggcgtgg
      541 gcatgacgct gactcccctc ccggccgcgc cacaccctcc cgaaccggcc gtgggcatcg
      601 cacggcgact gcgcggctgc gcctagatcc tacagttcct ggtcgcgacc gatgttctgc
      661 agctcgtcga cgatggcgcg cagctcggcg gcgcgggcgt gcatgcgcac gattggctct
      721 ccgttgtggt cgaggaagag gatgtcgaca tggtcgtcgg cgtcgaccag ctcggagccg
      781 ccgtagtcca gctcgacggt caggaccggg gtgatgcgct cgccgggctg gggcggctcg
      841 ccgggaggcg tgttggtggc ggcgtagccc cagtggtcga ccaggtcgac cagcgcgccg
      901 cgctcattgg gctcggcgcc gaggtagagc gagaggtcga tacggccgtc gctgaggtgg
      961 aggtagtagc cgtcctgggc gctctcgatg gcgatgtcct gcgggaacca gaggtcgtag
     1021 atgaccagcg ggcggacgac gtcctcttcg tcctcctcgt cggcctcgtc gatcgcgtcc
     1081 tcgaccgctt gatgctgcag cagggagtag tgcgccagcc cgtaggcgta gaggatctcg
     1141 gccaggcggg tgcgcagggc ggggttgaag gtgctgggga attcgtcttc gaggagccgc
     1201 tccagggtgc ggatcatgcg cccgtactct tccatctggc gcagctcacg attgacgaag
     1261 ccgcgggcgc cctggaaggc cgcgcggaag tcttcgtaga tgtcctcacc ggactgctca
     1321 ccgaggaagc gacggatttc ttcgaacagc tcctcgtcgt ccgacggtcc ctcgcgctcc
     1381 tgctctccgg acaacgtcat ctcccccgat cgtgtcgctc gcggcaccgt gcggcgccgc
     1441 caggggcaac aataccagag gtctagtatc tgggctagca ctgccaaatg cgggccatca
     1501 ccgctaggag aatggtgctg atgcagggct tattgggtgg cgttgcgcga ggggatgcgc
     1561 ggggtgggga cacgatcggc gcccttcgtg gtggcacgat ggcgctcggc gcccgagaat
     1621 cgaattctcg ggctgaatcc acgaagccca ctgaaggggc tgagtttgtc ggatagcctc
     1681 atacgtgatc gctgtagggg cagacccccg tgtctgccct gttcccccta cgcgtagcca
     1741 tcgaagacaa gggatgacgt cgtcaggggg cgacatcggt ggcgagcgac gagggccgga
     1801 ggcgggccac gagggcagac acggaggtct gcccctacag gggggggaga gatgccgtag
     1861 ccccggcagc ccagcccctt tagtgggctt cgcggattca gcgcgggaat tcgattccga
     1921 acggagccgc gcgaccacgg cgccgatgct ccagggaatg ccgcaaaccg accatcaatc
     1981 ctctcgacaa acgtcgaacg gggcgcgagc attcgcgccc cgtcctgcgt tggctgccgc
     2041 tggcgcggcc gagccgctct ggcctacttg ccgacctggt cggcgaactc ggcctcggtc
     2101 tcggtcaggc tctcgtcgat gatggtgacg atgcggtcca gctccgggta ggtgaccacc
     2161 aggggcggcg ccacgtggac gatgtcccag gtgcgcgtca gcatcccctt gtcgttgatc
     2221 cgcttgtcga gcgccttgac gaagcccgac tccttgctcc acttggcctt ggtgtccttg
     2281 ttctgcacga tctcgacgcc gcacatcaga cccaggccgc ggacatcgcc gacggtcggg
     2341 tgcgtgcgca gcccttcgag cttgtcgagg aggtagcggc ccttctcggc gctctgctcg
     2401 ggcaggtgct cggcctgcat gatctccagg ttcttgagcg ccgccgcgca ggccaccgcc
     2461 tggccgccga aggtgagcag gtgccccagc gccgtctcct tcttctcgcg gaagatctcg
     2521 aagacgcggt cgctgacggc gcaggccgcg atcggggcgt agcccgagga gaggcccttg
     2581 gccatggtca tcaagtccgg ctgcatgccg aagtgctcgg tggcgaacat cttgcccgag
     2641 cggccgaagc cgttgatgac ctcgtccatg atcaagagca cgccgtgctt gtcgcagatc
     2701 tcgcgcagcc gctgccagta cttgggtgag gggatatgca cgccgttgga ggtcgagatc
     2761 ggctcaccaa tgaccgcggc caccgtctcc ggtccctggt tgatgatctc ctgctccaca
     2821 tagttggcgc acatcacgtc gccggcctcg ccctccagcc cgaagtcgtt gcggtagtgg
     2881 ttgggcgagg gggtgtggta gacgccgtac atgaacggcc cgaagtaggt ctcgttgcgg
     2941 ctggcggtca ggctcatcgc gccgtgggtc atgccgtggt agctgccccg ccgggcgatg
     3001 atcttgtagc gcttggggaa gccgcgcatc acctggatct gcttggcgat cttgatcgcg
     3061 ctctcgaccg cttccgagcc acccgagcag aagtagacac ggttgagatc gccgggggta
     3121 atctgcgaga gcacgttggc caactgcacc gccgggaccg tggtgtagct ggcggaggag
     3181 gcgtaggcga tcttggcggc ctgctcggcc atcgcctcgc cgatctcggc acgcccgtgg
     3241 ccggcgttga ccacccagag gcctgagatg ccatcgatcc actcgcgccc gcgcacgtcg
     3301 accaacgtgc tgccgtgggc gcgctcaaag acatgcaacc cgtcctgctc ggccagccgg
     3361 atccactccg tggtctggat ccagacgtgc tcgagcgcct ggcgctccaa agcctccgat
     3421 ttggtcaggt ctttggcggt cgtcgcggtg tcgcccatga gctactcctc taaagctagc
     3481 ccgcgcttcc ccggcgccgg gcacgtcttg acaggtggct gggccgagcg tagcatacga
     3541 tccatctttg tggaaggcat cgcgccggga ttcggcgacg ttaaccacgc acgacgtgcc
     3601 tcaccccggt gaagccccgg ttgccgcccg gcggcaggcg aggaggtttc ccgaacacca
     3661 cgacatacga atctgccata cggaggatga tccacgaccg ggccatcgcc tcaatgcgac
     3721 acgtgaggtc gcgaggagga gtttgacgtt atgctggatc atacgtcgtc gccccgccgc
     3781 gcggacgaac cgcgcggccg cctgaccttc tcccgtcggc atgtcctgtt cgcgagtggc
     3841 gcgagcgcgc tggccgccct gctagcggcc tgcgggggct cttccgcccc ctcaacgccc
     3901 gccgcgaccg ccaacctcaa cgccccggcg cccacctcag cgcccaatct cggcctggcc
     3961 tccccggccg ctacgtcggc agcgacctcg gcggccacct ctgccacgac cgctgccgcc
     4021 agccccgccg ccggcggtgc cgagatcggc aagctggtgc tcaacaccga gccctacccc
     4081 aaatacacgg gcactccgaa gccgggcgat accttgacca tcatccgcaa tgaggacctg
     4141 agcgacctca accccgccgc gctcaactcc tactcgccct acaccttctc ctacgatccg
     4201 ctggtctgga tcgacgagta caccctcgac cccaagccct ggctggcgac ctcctgggag
     4261 gtgacgcccg acggcaagag ctacacctac aagctccgga acgacatcaa gtggcaggac
     4321 ggcaccccct tcacggccga cgatgtcgcc ttctcgatgg tcgtctaccg cgacgaccca
     4381 gagagcggcg tcgcgcgctt cttcccgctg atgaagcagg atccgaccgt cgtcgatgcc
     4441 cagacgattc gcttcgacct cagcgatccc tccggcgact gggtgctcaa tgccagcaac
     4501 cagttcatgc tgcagaagaa gcagttcggg gactactgga actccggcaa gggtgagaag
     4561 ggcgccaaga ccctcaaggg cttcccctac accgagcaga tgctgatcgg taccggacaa
     4621 tggaagcaga ccggctacgc acccggctcg gcgccgccct acctccagta cgaggccaat
     4681 cccaattact ttcagaacgc gccccacttc cagaagctga ttttcaagga ggtggacgag
     4741 gcgcaggccc gcatcaccgc ctggctcaac aacgagaccg acctcctctg gccgatcacc
     4801 gccaccgacg tcgatcaggt caaggggcag gacggctggc tctacagcgc ctacgccgtc
     4861 gccttcatgt gcgcctggat caacttcaag aaccccaagg tggtcaacgc cggcttcctc
     4921 aacgacaagg ccgtgcggca ggcgctctcg attggcatcg accgcgccgg cttcgcccag
     4981 tcggtcttcc acggcttcgt cgatcagctg aagatcggct cggtcgcctt cccctgggcc
     5041 tacaacacca cgctcaagag cccggaccac gacgccgcta aggccaagca gatgctggaa
     5101 gccgccggct acaagaccgg cagcgacggc aagctgcaag acaagagcgg caatccggtc
     5161 aagctcgtcg ccatcaccgc gaacaacaat cagtacccgg ttgataagat cgccgtctcg
     5221 gtccaggagg acttccgcca actcggcatc gacatgcaga tcgacacgct ggagccggcc
     5281 gcgctcaagg cgcgctggca gaagacgttc gactgggatc tctacttcta cagccgcatc
     5341 ctcttcgccg gcttcagcga ctacaactac tatcacagcg cctgggaccc gcgcaccaat
     5401 ccccagggcc gcgattttgg cgcctggaag aacaccgacg ccgacaaact gctcgaccag
     5461 atcatccgcg agcccgacct caagaagcag aaggatctcc tctggcagtt ccaggccgtc
     5521 atcgccgacg acatgccggc cttctggttc ggcttcccgc acgacctgat cctggtcaag
     5581 aagaacctcc agggctacca gccgaacgcc atgtggcagt actgggatac ctggaagctc
     5641 tggcgcacgt gatgccgcgg atgtaatctt ccgttttgcg ggatccggaa cgcgccggat
     5701 cccgcaccta ggacagggga cgagggatga cccgcttcgt cgtacggcgc ctggtgcagg
     5761 cgatcccgct gctcatcggc atctcgctcg tcgtcttctt gatgatctac ctgtcgccgg
     5821 gcgacgcctc taccttcgcg ctcagcgagc gcgccaacga ggcccaggtg caggcctacc
     5881 gccactatat gggactcgat ctgccctggt accggcagta cctgcgcctg gggggcaact
     5941 ggctacgcgg cgatctgggc acctcgttga tccagcggcg cccggtcagc accatcatcg
     6001 ccgagcgcct gccccgcacc gtcgagctgg tcgggatcgc cacgatcctg gccctgctgg
     6061 tcgccatccc gatcggcgtc ttctccgcca tacgccagta ctccgtctcc gaccacgtcg
     6121 tcaccgtggc gagcttcgtc ggcatcgcca tcccctcctt ctggctgggg atcatgctga
     6181 tcctgctctt ctcggtgaag ctccactggc tgccgaccgg cggcacccag acgatcggcc
     6241 agccctggag cctcgtcgat cacctcaagc atctgatcct gccggccgcc gtgctgacga
     6301 tcatccagac ggccgcctgg tcgcgctaca tgcgctcctc gatgctcgag gtgctggtgc
     6361 aggactacat gcgcaccgcc cgcgccaagg ggctgcccga gcgcctggtg ctgctgcgac
     6421 acgccatgcg caacgccatg cttccgatca tcaccctgct gggcctgagc ctgccgacgc
     6481 tcgtcggcgg cgcgatcatc accgagcaga tcttcagctg gaacgggctc ggcaagctcg
     6541 tcgtcgactc ggccagcaaa cgggatacgc cggtcgtgct ggccctggtc atgctctcgg
     6601 cgatcgcgat cgtggtcggc aacctgctgg cggacatcgg ctaccggctg atcgacccgc
     6661 gcattgaact gtagaaggac ggcagatgga cgagcaggcg gtcatcgcgc accccgaacc
     6721 gctcggcacg caccggctcg atctcgagct gcggcaaggg gcgcaaacct cggtcttgcg
     6781 gcgcttcggc cagaatcccc tggcgctggt cgcgctgctc gtgctggcgc tcctgacgct
     6841 cagcgcgttg accgcgccgc tctggctgcc gcgcgatcgc gtgctcgacc aggtgcgcag
     6901 cgccaccttc gcctcgccca gcctgcacgc gctcttcggg cgcgacgagt atggccgcga
     6961 cgccttcgcc cgggtcgtct acggcggccg ggtctcgctc tgggtctcct tctcctcggc
     7021 catcatcgcc acgctgctcg gcacggtgct gggcatgatc gccggctacc tcggcggctg
     7081 ggccgacagc gtcatcgatc gcctgatcga gctggtgctg accatcccct tcctgctcct
     7141 cttgatcctg gcggcctcga tggcccgccc ctcgcagacc agcatcatcc tgatcctcgg
     7201 cctcttcggc tggcccgtac tggcgcggct ggtgcgcggc gagttcatcg cgctgaggcg
     7261 gcgtgattat gtcgacgcgg cgcgcaccgc cggctgctcc tccacgcgga tcatcctgcg
     7321 ccacatcctg ccgaacgcga tggcgccgat catcgtgact ggcacgctga tcctggcgac
     7381 caacctgacc gccgaggcgg ccatcagctt cctcgggctg ggcatccagc cgccgacccc
     7441 gagctggggc aacctcttga ccaacgcaca gaactacgtc atcagtgcgc ccttcctggc
     7501 catcatcccc ggcctgatga tcttgatcac cgtcctctgc gtgaacgtgc tgggcgacgc
     7561 cctgcgcgac gcgctcgacc cgcgcctgcg tggtcgccac tgagccgcgc ttccgcgcac
     7621 ttgccgcggc tggccgcgaa tgctaccgtg tgcgggccga aaaccgacgg ttgagtgggc
     7681 gtgacgcatg agggagcggt gatggcaacc caggcggtgg acgagcggat tcgcgcgctg
     7741 atccaggagg cgggggtcgg cggcacggtc ggcgtcgcgg cgcacaagct cggcacgtcc
     7801 gagcagatca tgctcaaccc cgacacggtc ttccccaccg ccagcagctt caaggtcgtc
     7861 ctgctctaca ccctctatcg cctggccgac gcgggcacgg tcgacctcgg ggcgcgcgtc
     7921 gccctggagg agcggcaccg cgtgcccggc tccggcgtgc tgcaggacct cgacgccggg
     7981 gcggtgctga ccgtcaagga catcgccaca ttgatgatcg tcatgagcga caattccgcg
     8041 accgacatga cctacgacct ggtcggccgc gacgcggtcc agcagtcgct ggaccgcctg
     8101 gacatgccgc acacccacct gccgctcggc tgctggggca tcctggccgg gctgcgcgac
     8161 ctcgacccca acgaccccga cctcacctac gccgagctga agcgccggtt gggcgaggag
     8221 gagggctcgt gggattgtgc cgcgctggcg gagacgccgg agaataacgt ctccaccccg
     8281 cgcgacatgc tgcggctgat ggaggggatc aacgccggcg aggggctgag cgccggctcg
     8341 cgcgcggccg tcatcgacat cctgctgcgc cagaagatca gcgaccgcat cccggcgctg
     8401 ctgcccttcg gcgtgcgcgc ggcgcacaag accggctcgg tcaagggcgt gcgcaacgac
     8461 gtcgggctga tcttcgccgg ggagaccacc tacgcgctcg ccctgatgtc gaagggcgcc
     8521 caggatgagt acgaggtcac ccagcttctg gcccgtatct cgcgcgccat ctacgacgcc
     8581 ttcgccggcg cgcccgccac ggacccggcc tagcgcggtc cgcgcgggcg gctgagggga
     8641 gcgcgcgtgc tggaccggag cggaggcgcg cgcggcggcg agctatcgca gcgggacctc
     8701 gtccccttct acctgggcgt gctcagcggg ccgatgggtt ccggcccggt catcgtcctc
     8761 ttcgtggtgc tgatggacgc cttcggcgtc ggccgtggcg cgctctcgct ggccgtcacc
     8821 gcctacatga tccccttcgc cacggtgcag ttgatctccg gcggcatctc cgacctcacc
     8881 tcgcgccgct catccttgct gggcggcttc ggcgccttcg gggcggccac catcttcgcc
     8941 gggctcgcgc ccagcttcgg gctcttcctg ctggcgcagg cgctgcaggg cgcggccaac
     9001 gccttcacca ccccgctggt gctggccacg ctgggcgaca tcacccacgg gcgccagctc
     9061 tcgcgcgccg tcgggctctt ctccaccgcc aacatcgccg gcttcctggc cgcgccgctg
     9121 gtggcgggct tcctcggcgg catcagctgg cgcgcgccgt atctcgcggt cgggctctac
     9181 acctgggcct tcgccgcctg gtattggttc tggttccggc gccacggcgc ctcggtgccg
     9241 ccccgcccgc gcagcggcag cctgggcgcc gacagccgcc gcatcctgcg cgcgctcggc
     9301 ctgacggtgg cgctgctggc ggccatctcc ttctgctaca gcgtggccac ccgcggcgcc
     9361 tcctacctct tcgccgacta cctccagcac cgctggggca gttcggtcga ggccgcgggc
     9421 gtgatcctgg cgaccttcgg gctgggcggg ctgctgctgg ggccgctcag cggcgacgtg
     9481 atcacgcgga tcggcctcta ccgcggcgcc gcgctgagca tgctcggcgt ggcgctctcc
     9541 ttgatcctgc tggcgctcgc gccctcgccg ctggccttcg ccggggccaa cttgctctac
     9601 ggtgtggcgg ccgtctacgc ctggaccgcg ctcaacatgc tggtggtgga ggcggtcccg
     9661 gcgcatcggg gcactgtctc ctcgatcttc ggcgcggcgc gcttctacgc catgggcgtg
     9721 gcgccgcttt ggttcacccc gctctatcag tcgctcggcg cgcgctcgat ctaccccgtc
     9781 tcggccgtct tcgccctggc cctcctgccg cccctcctgc gcctgcgcgg ccaccacgcg
     9841 cgcgtgatac ccggggcaac ggccgacgac ctggcgcgcc gctgacggcc gcgcgatctt
     9901 cccggagaca acggttcacg atgctggagg acagccgcga gccaggcggt cgtcagggac
     9961 ggcaccttct ccggtgcaag tcaccggtcc gaagatgccg tcccgcgcgt aaacgagttg
    10021 tggtttttcc aacgaggtgg acgttcgcga cgcgcgacgg aggcggcaca gcgtctgtcg
    10081 accggaagat cgcggcgttg tcagcctacc gcacccagta cccgctgcgg ccggacacgc
    10141 tgccgcgcgc gctgctgctg gagatgttcg gcatcgaata cttcgtccag gcccgcccct
    10201 gccgcgccgt cgagtccgag atcctgcgcg tccccgcggc gatcgaacgc tagcgcggct
    10261 tcgcctgtcc tccgacgacg cctctccgct cgtccttgac gggtggatcg ctgcgtctgc
    10321 cctggccgcc cacggcctcg gcggttgtat gcttcacgcc ccgagcgagg gtgccggccg
    10381 gatcgagcgg cagcgctgac ggggcttcgg agaggagccc ggcgccgcct acaggctgaa
    10441 agggacggga actgacattg gcaccgacag gaaagccgca gcgcctctac ctccttcagc
    10501 tcggcaccac caccatcccc ggcgcgcgag ggccgctcag catgtcgtcg ggctgctacc
    10561 tggtgcagac gagcgccgac gagcacatcc tgatcgacag cggcctcccg tccgactaca
    10621 cgccgccggc gggcgcgccc gccgcggaga accaacggga tgtgctccag catctggcga
    10681 cgctcgggtt ggcgccggga gacatcgacc tggtcatctg aacgcacttt gatatcgacc
    10741 acgtcggcta tcaccgctcc ttcccgtccg ccgcgttcgt cgtgcagcgg gagcactacg
    10801 agctggcgcg cgccggccat gcgcgcttcg cggccgcgac ttcatactgg gatgatccca
    10861 ggctccacta ccgcatgctc gacggtgata ccgagctgct gccggggttg acgctgctca
    10921 agacgcccgg gcacgcgccg gctcatcagt cggtgctggt gcgcctgccc cagaccggct
    10981 cggtgttgct ggcgatcgac gcggtcatgt tcgcgcggca gttcacggcg gatcgggccg
    11041 cctggccgat ggacgacaat gaggcggagc tgcgcgccag cacccggaag ctactcgacc
    11101 tcgtggagcg cgagcaggtg gcgctcgtcg tcttcgggca cgatggcgag cagtggcaag
    11161 cgctgaagaa ggcgcccgag tattacgcct agcgctgccc caccggcgtg cccggcacga
    11221 tcagcgacag tctgcggagc cgctcagtcc gccgcgtgcg tcaggcggca gaagcaggag
    11281 gcgtcgatgg gcacgtcggc gcgggcgctg gccagggtca ggcgctggct gaccgggccg
    11341 cagaggtcgt gcttgcccag ccgcatgacg cactcgtggt agccgtgcag gctccagacg
    11401 ttgcccgggt gctggtgcgc gcgggccagg ctgccgccca gccccaggtc ggccttgtag
    11461 accgcctcgg cctcctccac ccggtcttgc tccaagagca gcgcgcccag cgcgtgccgc
    11521 gccggctgca tccagcccca gggctcgctg tagagcagct catcgtagag ccgcaccgac
    11581 tcgcgcaggt gggcgaaggc gtcgtcgtag ttgcccttgc ggtactccag ctcgccggcc
    11641 agcatcgcgc ccgcgatcag gagcaggtcg acgcagcggt tcccctgatg ccagcgagtc
    11701 tcgggcacgc gcgcggcggc ggccacgaac agccgctgct gctcctcggc ggccgggatc
    11761 cggccggtca cggcgtaggc gattcccttg gcgtagtggg tcatggcggt ggtgtagcag
    11821 agcagctcgg ggtcgtccgg cagcggcgtg tcgatgatct cctgccagcg gccaaagcgg
    11881 atcaagacat gcagcttggc cgggatgaag ccctccagcc ggtcggccat cacgatcggc
    11941 gtctgctgct ccagcagctg gcgcgggatg atgtcgagaa tggcgtcggc cgattcgagc
    12001 gcgatctccg actgggccag gaacatggcc gcgtagagcc gcacgatgtg gttgtgcagg
    12061 cgcgagaagg tgtacatgtt catcgtgccc tggcgcgccg cgtacttgtt gtcggccgcg
    12121 atcgccgccg tgttgcaggt gaccgcgctg tagtagctgc cgcagagggt gtcgatgtgg
    12181 gtcggcatgt ggcgcaggtg gcctgagtcg ggcacgagat cacgcagcca gtcggccgcc
    12241 cgcagcgcct gctccggctc gggcgacatc tccatcaggt ggatgtactg gtgcagcacg
    12301 ccggggtgcc gctggctggc cgggtccgcc agcgcccgct ccagcaccgc gcgcgcctcc
    12361 agggtgctgg cgccctcggc cggctggccg gtcggcagat cccagagctg ccagggggtg
    12421 cggttgagga gcgcctcggc gaagaacgcg gccacgtcca ggtcgtccgc gaagtcgtgg
    12481 tagacggcgc gcatggcgtc ggcgtagtcg tcgttccaga cgcggcaatc ctccaccggc
    12541 acggcttgcg ggtagcgggc cgtgagcgcg gtgatcaacg cctgatcggc cgggttggca
    12601 ccctcgatca acgccagcgc gcgctggctg gccgcgtggc aggtggcgac catcgcctgg
    12661 cgggccgggt cggtcagcat gtcccagcgc aggttgtagt aggggccggc ggcgtaggcc
    12721 acgccccagt gggccatggc gcaggccggg tcgtgctcga gggcgcgctc gaaacagcgc
    12781 tgcgcctcct cgcggttgaa gccgtaggtc cagttgaggc cgcggtcgaa ccagcgctgc
    12841 gcctccggcg acgaggtggt gatggtgcgg ccgtgcgggc cgaggtcgta gtcgtagtcg
    12901 tcgtcgtagc tcgccattgg tctgctcctc gcttggccac tcgacccggc cagccgccga
    12961 gccaccgcgc tcgaactcag gatggcgatc atgctagccg ctgcgacgaa cgaatgcaag
    13021 ctgccgggct aacgctgaaa cgagaagcgt gtggggcggc tgtttctcga tatagtatac
    13081 gtgcggaggt ccgctccgga aggcggaggt gatcgagacg gttcccgcca cactgcacga
    13141 tttcttcgtc gccagcagcg gcgccagcgc cgccttgatc ggcttgctct tcgtcgccgt
    13201 cagcgtggcg cccgagcgcg tctttggccc cggcgcctcc ggcgagcgga cctaccgcgc
    13261 cggcagcgcc ttcttcgtcc tcagcaacgg cctcttcatc tcgctgggcg cgctgctgcc
    13321 gcagttgaac ctgggcgaca tcctcgtgct ggtcggcgcc ggcggtctcc tcaacattct
    13381 cctcttgatc atcgagctgt ggcagcgagg cgcgcgcccg gcggggcacg ggctgctgct
    13441 ggcactcggg agcgtggcgc tctacgccgg agaagtgcgc ttcggaatcg ggctactgcg
    13501 ccaccccaac gatcagcgtg cgatcgacgc ggtgacgtac atgctgctgg ccggctacgt
    13561 gatcggcctg gctcgagcct ggcaactggt cagcgggc
//
LOCUS       DASUBI010000835         6236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1329522, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000835 DASUBI010000000
VERSION     DASUBI010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329522"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFU72_16480"
     CDS             complement(<1..397)
                     /locus_tag="VFU72_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425141.1"
                     /translation="MSAATLTVRSSGAPVDKLRFSNRDQPGTPCVYTPDRLEVTVDLG
                     AVAAGEPPAERVRLLLPPGWLCDRATERQVGSDRWLTWTVFPKETPEEFTPAITAELI
                     AGAATVQRLGQSLAVAIEARSQFNPAKDVL"
     gene            complement(402..884)
                     /gene="nrdR"
                     /locus_tag="VFU72_16485"
     CDS             complement(402..884)
                     /gene="nrdR"
                     /locus_tag="VFU72_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU5425142.1"
                     /translation="MRCPFCQARDSRVVDSRELTGGESIRRRRECVACGRRFTTYERV
                     ESGGLIVVKRDGRREEFDPRKLREKLRIALTKRPISQEEIDRVVAQVEKELLERGTVE
                     VPSTAIGEIALRELKRLDHVAYIRFASVYRDFADLEDLRRELETLNGVSRSGAGDPAA
                     "
     gene            complement(886..1998)
                     /gene="ftsZ"
                     /locus_tag="VFU72_16490"
     CDS             complement(886..1998)
                     /gene="ftsZ"
                     /locus_tag="VFU72_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5425143.1"
                     /translation="MFNVRDDGYASSFARIKVLGVGGGGGNAINRMIAAGVEGVEFIA
                     VNTDAQALLNQLAPMTVRIGDKLTKGLGAGGRPEVGERAAEETVEELADSVRGADMVF
                     ITAGMGGGTGTGASPIVAKLARETGALTVGVVTKPFDFEGAKRRRVADEGVAALKEHV
                     DALITIPNQRLISLVDPKTPFSEAFRLADDVLRQGIQGISDLIVKPGLINLDFADVKT
                     IMRDAGSALMAIGRGTGENRTVDAARMAIESPLLEMSIDGAVGVLYNIIGGQDLSLSE
                     INEAAEVIRAAADEDAEIIFGATTDDAMGKDVQITLIATGFQGGASSRRAQPGRRFQA
                     PASGMGGSQQAAPSRIPVGSDDEWSEPAILRFLRER"
     gene            complement(2326..3054)
                     /locus_tag="VFU72_16495"
     CDS             complement(2326..3054)
                     /locus_tag="VFU72_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5425144.1"
                     /translation="MSRATTRRSRRQRFHGAVRALIVTGRLPAFMLSVGLAVFVVGFL
                     TSHDFVVRSVVVEGNQAAFAGSIVDASGALGQPIFRLDTAEVARRVSAHPAVASASVS
                     ARFPDTVVVHLTERVPVLVWQVGTRAVLVDDHGWVIAEAYDAKLPRVFEAQGDLPAIG
                     SQLPPQRVQAALSITSRLGAALSSLQYDPATGLTAQLADSRTVVLGEADRLPLKLSVL
                     EAALKLPDHWSRLDVREPDRPYYQ"
     gene            complement(3086..4195)
                     /locus_tag="VFU72_16500"
     CDS             complement(3086..4195)
                     /locus_tag="VFU72_16500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEU5425145.1"
                     /translation="MRRRLRVGVIFGGRSGEHDVSLRSAQAVMHALEDGGFTVVPIGV
                     TREGQWLRGGDPLAQLAAQSHLPLAATANVTANGHGAVVPARQEPLSLESAQEALSGE
                     IDVLFPVLHGPQGEDGTVQGFLELSDIPYVGSGVLASSLGMDKIVSRQLFAQVGLPLA
                     RWTWLLRRDWRRDPDAVAARIEREIGFPCFTKPANLGSSVGISKVHHAGELAAALDDA
                     AHWDRKIIVEEGLDARELEVSVLGNDDPVCSVVGEIVPANEFYDYDAKYVDEDSQLLI
                     PAPIPVELADELRAMAVRAFKALDGAGLARVDFFLERPSGRLYLNELNTIPGFTATSM
                     YPRLWEASGLPFPELVTRLVELAVERYGEHGPVQG"
     gene            complement(4197..5138)
                     /gene="murB"
                     /locus_tag="VFU72_16505"
     CDS             complement(4197..5138)
                     /gene="murB"
                     /locus_tag="VFU72_16505"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5425146.1"
                     /translation="MPETIALATPAGTLKLRRGQPLSLHTTFRIGGPADVLVRVANAG
                     EALAALEWADREGLPVTAIGGGSNLLVGDGGVRGLVLLVRAPGKDVAGSYTVEELGDH
                     VRLTVPAQAPISWLGHLSARHGWQGMDWAAGLPGQVGGAVVNNAGAHGGETKDHLESI
                     DVLDLARGAVERWPRARLEPAYRHTVLKAAPRPRRWLVLGATFLLPHGDAAELTARAE
                     EHARWRRQAQPTGACAGSVFTNPEGTYAGYLIDQAGLKGRQVGGVKVSELHGNFLVNT
                     GSGTAAEVRELIAQIQAEVRRQFGIALVPEIEQIGDE"
     gene            complement(5138..>6236)
                     /locus_tag="VFU72_16510"
     CDS             complement(5138..>6236)
                     /locus_tag="VFU72_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5425147.1"
                     /translation="KRGAVLGLLCNERVCVAVAGTHGKSTTCGMLALALERAGEAPSF
                     AVGAVVRQLGSNARLGAGPIFVAEADEFDYSFLWLSPDVAVINSIEPDHPDIFPTFEA
                     VEEAFDRFAQRIRPGGTLVISADDPGCRALVSRRPWPEGTDIVTFGLSEHADWRIERV
                     AGRDHFHGPQGTVRLRLAVPGIHNRRNAAAALAAGAALGIAPARLAAGLEEFTGVGRR
                     FERRGEAGGVTVIDDYAHHPTEIRVNIVAARERYPAARVVALFQPHTFSRTRQLLDEF
                     AQALSLADEVVLAPIYAAREQDDLGVSSADIAARVAGPCVALADSVEEAGRLGAELAR
                     PGDVLLVLGAGDIPRAGEIALRLLEQSTKGS"
BASE COUNT          910 a         2336 c         2109 g          881 t
ORIGIN      
        1 gtagcacgtc cttggccggg ttgaactggc tgcgcgcctc aatcgccacc gcgagcgact
       61 ggcccaaccg ctggaccgtc gccgcgccgg cgatcagctc ggccgtgatg gccggcgtga
      121 attcctccgg cgtctctttc gggaagacgg tccaggtcag ccagcgatcg ctgcccacct
      181 gccgctcggt ggcccgatcg cagagccagc cgggcggcaa gagcagccgc acgcgctcgg
      241 ccggcggctc gccggcagcc accgcgccga gatcgacggt gacctccagc cgatcggggg
      301 tgtagacgca cggagtgccg ggctggtccc gattgctgaa gcggagcttg tcgaccgggg
      361 caccgctgct gcgaacggtc agcgtggctg cggacatgga tctaggccgc cgggtcgccg
      421 gcccctgacc gggacacgcc gttgagggtc tccagctcgc ggcgcagatc ctccagatcc
      481 gcgaagtcgc ggtagaccga ggcgaaacga atgtaggcca cgtggtcgag ccgcttcagc
      541 tcgcgcagcg cgatctcgcc gatggctgtg ctcggcacct cgaccgtgcc gcgctccagc
      601 agctccttct cgacctgggc caccacgcga tcgatctcct cctgcgagat cggccgcttg
      661 gtcagcgcga tgcgcagctt ctcgcgcagc ttgcgcggat cgaactcctc gcggcggcca
      721 tcgcgcttga ccacgatcaa cccacccgac tcgacccgct cgtaggtggt gaagcgccgc
      781 ccgcaggcca cgcactcgcg ccgtcgccgg atgctctcgc cgccggtcag ctcccgcgag
      841 tcgacgacgc gcgagtcgcg cgcctggcag aacgggcaac gcatctcagc gctcgcgcag
      901 gaagcgcagg atcgccggct cggaccactc gtcgtcggag ccgaccggga tgcgcgacgg
      961 cgcggcctgc tggctgccgc ccatgccgct ggccggcgcc tggaagcgcc gccccggctg
     1021 agcccggcgc gaactggcac cgccctggaa gccggtcgcg atcagggtga tctgcacgtc
     1081 cttgcccatg gcgtcgtcgg tggtggcgcc gaagatgatc tcggcgtcct cgtcggccgc
     1141 ggcccgaatc acctcggctg cctcgttgat ctccgagagc gacagatcct gtccgccgat
     1201 gatgttgtag aggacgccca ccgcgccgtc gatgctcatc tccagcagcg ggctttcgat
     1261 cgccatgcgc gccgcgtcga ccgtgcggtt ctcgccggtg ccgcggccga tcgccatcag
     1321 cgccgagccg gcgtcgcgca tgatcgtctt gacatcggcg aagtcgaggt tgatcaggcc
     1381 cggcttgacg atcaggtcag agatgccctg gatgccttgc cgcagcacgt cgtcggccag
     1441 ccggaaggct tcggagaagg gagtcttggg atcgaccagc gagatcagcc gctggttggg
     1501 aatcgtgatc agcgcgtcga cgtgctcctt gagcgcggcc acgccctcat cggccacccg
     1561 acggcgcttg gcgccctcga agtcgaaggg cttggttacc acgccgaccg tcagcgcgcc
     1621 cgtctcgcgc gccagcttgg ccacgatcgg cgaggcgccg gtgccggtgc cgccgcccat
     1681 gcccgccgtg atgaagacca tgtcggcgcc gcgcaccgaa tccgccagct cctccaccgt
     1741 ctcctcggcg gcgcgctcgc cgacctcggg ccgcccgccc gcgccgagcc ccttggtcag
     1801 cttgtcgccg atgcgtacgg tcatcggcgc caattggttc aggagcgcct gggcatccgt
     1861 gttcaccgcg atgaactcga ccccctcgac gcccgccgcg atcatccggt tgatggcgtt
     1921 ccccccacca ccgccgacac cgagcacttt gatccgcgcg aacgagctcg cgtagccgtc
     1981 atcacgaacg ttgaacatct accccgcctc cttcggccta ccttgcggcg accacgtcga
     2041 acaccccatc actcgttccg catgctcgca aaaatgtgga aaaagtatgc ctcacccaat
     2101 gggttcgccg caaaagcggc cgatcgtcga tgattccgct ctccccggca ggttaccggt
     2161 tgcgctctca agccgcctta ccgagcctcc ccaggctcgg aacgttgaat gtcgcgggaa
     2221 tccggcgctc ggcgccgcct ccagtatagc acccatccgc tggcctgtac gtgctccggg
     2281 tcctaattcc cggcctattc ggacagcgcg tacgagcaca tcggttcact gatagtacgg
     2341 acgatccggc tcgcgcacgt ccaggcgcga ccagtgatcc ggcagcttca gcgccgcctc
     2401 gagcacgctc agcttgagcg gcaggcggtc ggcctcgccc agcacgaccg tacggctgtc
     2461 ggcaagctgt gccgtcaacc cggtcgccgg gtcgtactgc agcgagctca gcgcggcgcc
     2521 cagccggctg gtaatggaca gcgcggcctg aacccgctgc ggcgggagct ggctgccgat
     2581 cgccggcaga tcgccctgcg cctcgaagac gcgcggcagc ttggcgtcgt aggcctccgc
     2641 gatcacccag ccgtggtcgt cgaccagcac cgcgcgagtt cccacctgcc agaccagcac
     2701 cggcacgcgc tcggtcaggt gcacgaccac ggtatcgggg aagcgcgccg agaccgaggc
     2761 cgaggccacc gccgggtggg ccgaaacccg gcgcgccacc tccgccgtgt cgagccggaa
     2821 gatcggctga cccagcgccc cgctggcgtc gacgatcgag ccggcgaagg ccgcctgatt
     2881 cccctccacc accactgagc gcaccacgaa gtcgtgcgat gtcaggaagc cgaccacgaa
     2941 gacggccagc ccgacactga gcatgaaggc cggcaggcgg ccggtcacga tcagcgcgcg
     3001 caccgcgccg tggaagcgct ggcggcggct gcgccgcgtg gtggcgcggc tcacggggct
     3061 cggttgacgc gcgctcaccc tgccgctacc cctgcaccgg gccgtgctcg ccgtagcgct
     3121 cgaccgccag ctcgaccagc cgcgtgacca gctccgggaa cgggagcccg ctggcctccc
     3181 agagccgggg gtacatgctg gtggcggtga agccagggat cgtgttcagc tcgttcaggt
     3241 agagccggcc gctgggccgc tccaggaaga agtcaacgcg cgccaggccg gcgccgtcga
     3301 gcgccttgaa ggcgcgcacc gccatggcgc gcagctcgtc ggccagctcg accgggatcg
     3361 gcgccgggat caagagctgc gaatcctcgt ccacgtactt ggcatcgtag tcgtagaact
     3421 cgttggccgg cacgatctcg cccaccaccg agcagaccgg gtcgtcgttg ccgagcacgc
     3481 tgacttcaag ctcgcgcgcg tcgagccctt cttcgacgat gatcttgcgg tcccagtgcg
     3541 ccgcgtcatc gagcgcggcc gcgagctcgc ccgcgtgatg caccttggag atgccgacgc
     3601 tggagccgag attggccggt ttcgtgaagc aggggaagcc gatctcgcgc tcgatgcgcg
     3661 cggcgaccgc gtccgggtca cgccgccagt cgcgccgcag cagccaggtc cagcgcgcta
     3721 gcggcaggcc cacctgggcg aagagctggc gagacacgat cttgtccata cccagcgacg
     3781 acgccagcac gcccgagccg acgtagggga tgtcggacag ctccaggaag ccctgcaccg
     3841 tgccgtcctc accctgcggg ccgtgcagca ccgggaagag gacgtcgatc tcgccggaga
     3901 gcgcctcctg ggccgactcc agcgacagtg gctcctggcg ggccggcacg accgcgccgt
     3961 ggccgttggc ggtcacgttg gcggtcgcgg ccagcggcag gtgcgattgt gccgccagct
     4021 gggccagtgg gtcgccgccg cgcagccact gcccctcgcg cgtgacgccg atcggcacga
     4081 cggtgaagcc gccgtcctcc aacgcgtgca tcacggcctg cgccgagcgt agggagacat
     4141 cgtgctcgcc cgagcggccg ccgaagatca caccgacacg tagtcgccgc cgcatgttac
     4201 tcgtctccaa tctgctcgat ctcgggcacc agcgcgatac cgaactgccg ccgcacttcg
     4261 gcctgaatct gcgcgatcag ctcgcgcacc tcggccgccg tcccgctacc ggtgttgacc
     4321 aggaagttgc cgtgcagctc cgagaccttg acgccgccga cctggcgccc cttgaggccc
     4381 gcctggtcga tcaggtagcc ggcgtaggtg ccctcggggt tggtgaagac cgagccggcg
     4441 caggcgcccg tcggttgcgc ctggcgccgc cagcgcgcgt gctcctcggc gcgcgccgtc
     4501 agctcggcgg cgtcgccgtg gggcagcagg aaggtggcgc cgagcaccag ccagcggcgc
     4561 gggcgcggcg cggccttgag caccgtgtgg cggtaggccg gctccagccg cgcgcgcggc
     4621 cagcgctcga ccgcgccgcg cgccaggtcg agcacgtcga ttgactccag gtggtccttg
     4681 gtctcgccgc cgtgcgcccc ggcgttgttg accaccgccc cgccaacctg gccgggcaag
     4741 cccgccgccc aatccatccc ctgccagccg tggcgggcgc tcaggtgacc cagccaggag
     4801 atcggcgcct gggccggcac ggtcagccgc acgtggtcgc ccagctcctc gaccgtgtag
     4861 ctcccggcga cgtccttgcc cggcgcgcgc accagcagca ccagcccgcg caccccaccg
     4921 tcgccgacca gcaggttgct gccgccgccg atggccgtca ccggcagccc ctcgcggtcg
     4981 gcccactcca gcgccgccaa cgcctcgccc gcgttggcca cgcgcaccag cacgtcggct
     5041 ggtccgccga tgcggaaggt ggtgtggagc gagagcggct ggccgcggcg gagcttcagg
     5101 gtgccggccg gcgtcgccag cgcgatggtc tcaggcatca cgatcccttc gtggactgtt
     5161 ccagcaaccg cagagcgatc tcgccggcac gcgggatatc tcccgccccc agcaccagca
     5221 gcacgtcccc tggccgcgcc agctccgcac ccagccgccc ggcctcctcg accgaatcgg
     5281 ccagcgccac gcatggtccg gcgacgcgcg cggcgatatc ggccgagctg acgcccaggt
     5341 cgtcctgctc gcgcgccgcg tagatcggcg ccagcactac ctcgtcggcc agcgacagcg
     5401 cctgggcgaa ctcgtcgaga agctggcgcg tgcggctgaa ggtgtgcggc tgaaagagtg
     5461 ccacaacgcg cgcggccggg tagcgctcgc gcgccgccac gatgttgacg cgaatctcgg
     5521 tcggatggtg ggcgtagtca tcgatgacgg tgacgccgcc cgcctcgccg cgccgctcga
     5581 agcgccgccc gacgccggtg aactcctcca gccccgccgc caggcgcgcg ggcgcgatcc
     5641 cgagcgccgc ccccgccgcc agcgccgccg ccgcgttgcg ccggttgtgg atgcccggca
     5701 ccgccaggcg cagccgcacg gtcccctgcg gaccgtggaa gtggtcgcgc ccggccacgc
     5761 gctcgatgcg ccagtccgcg tgctcgctca gcccgaaggt gacaatgtcc gttccctccg
     5821 gccaggggcg ccgggagacg agcgcgcggc agccggggtc atcggcgctg atgaccagcg
     5881 tgccgcccgg ccggatgcgc tgggcgaagc ggtcgaaggc ctcctcgacg gcttcgaagg
     5941 tggggaagat gtcagggtgg tccggctcga tgctgttgat cacggccacg tcggggctga
     6001 gccagaggaa gctgtaatcg aactcatccg cctcggccac gaagatgggc cccgcgccca
     6061 ggcgcgcgtt gctgccgagc tgcctgacga ccgccccgac cgcgaaggag ggcgcctccc
     6121 cggcgcgctc caacgccagc gccagcatgc cgcaggtggt cgacttgccg tgcgtgccgg
     6181 ccacggcgac gcagacgcgc tcgttgcaga gcagcccgag caccgcgccg cgcttg
//
LOCUS       DASUBI010000836         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_40174, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000836 DASUBI010000000
VERSION     DASUBI010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40174"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFU72_16515"
     CDS             complement(<1..1454)
                     /locus_tag="VFU72_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425148.1"
                     /translation="MNEQRIRRELRRLAAREVPEADLWPAIRARLARRDALRRPGTGF
                     ARTAGSPERGAPAIESEFTWSERDRPRGAATRWAALLAGAVSLVLVAVLLSGVFGGRK
                     TVAPGATSVSVAIGVGATPSPTAGVTATPTFAATVVPQVLTPPYPTPSTCPVTPFLTS
                     APFGTNYTTTWLGYGQLFAGLDRAYGGHWYAGGLKVMWMRLQGGTLSITGTRLDGPAP
                     PLKADIPDGYGPAGIQVSEIDFPVSGCWSVTGMAGDSVVSFVVYAYPIGCRDSGWAHD
                     PATPQPCAPPGAISPAPVSVPASCPVTRPPGTAFVPPAPYPAQLGGGDFWYGSESLWA
                     LLPASGSLEVVPQDSGAYVAKLLWFHQGYDWQTEPQPPLKVSGHRLDAPAMTLDAIIG
                     SGFSAELHSFMIANLRFPAPGCWQVDADHLGTKLTVVVWVPAPPNIPNSTPELVVPLL
                     LVGLWRARRRGVAVLRAVDEAAEGLHRRQFRVLAR"
     gene            complement(1447..2031)
                     /locus_tag="VFU72_16520"
     CDS             complement(1447..2031)
                     /locus_tag="VFU72_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5425149.1"
                     /translation="MVVSGSEREAIERLRRGDIGGLEPLVRRYQLQAVRAATLITRDR
                     ALAEDIVQGAFIRAYERIAQFDATRPFGPWFLRGVVNDALKAARRERRQQPLEDDWGG
                     GAALDGLEAEPQRLAELLETSRSLQQALDQLPAEQRAAIVLRYYLDLTEQETARRLGS
                     PLGTIKWRLHAARARLRHLLGTDDPTTTRSVPDE"
     gene            2181..3131
                     /locus_tag="VFU72_16525"
     CDS             2181..3131
                     /locus_tag="VFU72_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5425150.1"
                     /translation="MVEFGDILAAREAISGEVRQTPVFSATRIGQRAGVRLQLKAELL
                     QKTGSFKARGALNAILQTPRAQLERGVIAVSAGNHAQGLAWAARQAGVRCVIVMAASA
                     SRTKIAATEGYGAEVVLVEGGVTAAFERAAELERAEGLVNIHGFDNPLIIAGQGTVGL
                     EIMEQVPGVEVIVCPVGGGGLISGVALAAKSLKPSVRVYGVEPEGASAMRQSWDRGEP
                     VTLSSVNTIADGLAPPMAGQLTYPLTRQYVDDIVTLSDEEIVVGLRAVLTEAKLYAEP
                     SGAAATAALLAGKIPHEPGALTVAIVSGGNMDLERLKTLI"
     gene            complement(3284..4606)
                     /locus_tag="VFU72_16530"
     CDS             complement(3284..4606)
                     /locus_tag="VFU72_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425151.1"
                     /translation="MRVVYPNPHPQQRVTALLAALESRELQRLGLAVLLAAGAGWLVV
                     LALLLRHVIFVSNDSLNNYAHVWYVSRRLWHHGRIPMHMPLLEHGEAYAFPYSFVPWL
                     SAALLRPLLGDWVVTLWLALGAVGTLAASFWALPELRRVWPAAAVLLNSALLASLLLG
                     QLPFLWASALLLAAVGAWRRDHFWWAIILGGLAQATHAPIVLPLAGLLVLAWLRWEPR
                     PDRLLVAYGTSLLLAAPAVWFALASPVTLESSFGTIAANFFGTLAERAPVVALPLLLA
                     ALQHRRPRLAPAACAAVLALGIATLPLLHTAQAARALERRPNTSLLAFIASPAFQPGQ
                     TYRFLRVGDDKVGMYELIQHGARLDSSFFPESVHHGSFVNADEYAAFLRQRGVDEVLI
                     YQSDTAAYHSNEQQLLGRLTKSQYGGVWAEHQLHTADYDLYVIHHARG"
     gene            4639..>4923
                     /locus_tag="VFU72_16535"
     CDS             4639..>4923
                     /locus_tag="VFU72_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425152.1"
                     /translation="MDRNAQMIPNRWQQVDRWIGGAALAALLLPGLALAPGRGNLLPP
                     LLAVLAAVAGAFLANRFTPRPRGGLALLGALMVALAAWLAAQIHFALTAYL"
BASE COUNT          751 a         1735 c         1790 g          647 t
ORIGIN      
        1 cgcgcgagaa cgcgaaattg acgtcgatga agtccttcgg cagcttcatc aacggcccga
       61 agtacggcaa ctcctcggcg acgcgcgcgc cacaacccca ccagcagcag tggcacgacc
      121 agctccggcg tcgagttcgg aatgttcggc ggggccggca cccagacgac cacggttagt
      181 ttcgtcccca ggtggtcggc atcgacctgc cagcagcccg gcgccgggaa acgcaggttg
      241 gcgatcatga aggagtgcag ctcggcgctg aagccgctgc cgatgatcgc gtccagcgtc
      301 atggccggcg cgtcgagccg gtggccgctg accttcagcg gcggctgcgg ctcggtctgc
      361 cagtcgtagc cctggtggaa ccagagcagc ttggccacat acgctccgga gtcctgcggc
      421 acaacctcca ggctgccgct cgccggcagc agcgcccaga gcgactccga gccgtaccag
      481 aagtccccgc cgcccagctg cgccggatag ggcgccggcg gcacgaaggc agtgcccggc
      541 ggccgggtga cggggcagga tgccggaaca ctcaccgggg ccggcgagat ggcaccaggc
      601 ggcgcacagg gctgcggcgt ggccgggtcg tgcgcccagc cggaatcgcg gcagcctatc
      661 gggtaagcat agaccacgaa gctgacgacg ctgtcgcccg ccatgccggt gacggaccag
      721 cagccggaaa ccgggaaatc gatctcgctg acctggatgc cggccgggcc gtagccgtca
      781 gggatgtcgg ccttgagcgg cggggcggga ccgtcgaggc gcgtgccggt gatgctcagc
      841 gtgccgccct gcagccgcat ccacatcacc ttcaatcccc cggcgtacca gtggccgccg
      901 taggcgcggt cgagcccggc gaagagctgg ccgtaaccga gccaggtcgt ggtgtagttg
      961 gtgccgaagg gcgcactggt gaggaagggc gtcaccgggc aggtggacgg cgttgggtag
     1021 ggcggcgtga gcacctgcgg cacgaccgtc gcggcgaagg ttggcgtcgc tgttacgccg
     1081 gccgtgggac tcggcgtcgc gccgactcca atggcgacgg acacgctggt ggctccggga
     1141 gcgacagtct tgcgtccgcc gaagacaccc gagagcagca ccgccaccag caccaaggag
     1201 actgctcccg ccaacagcgc ggcccagcgc gtggcggcgc cgcgcgggcg gtcgcgctcg
     1261 ctccaggtga actcgctctc gatagccggc gcgccgcgct cggggcttcc cgccgtgcgc
     1321 gcgaagccgg tgcccggtcg gcgcagcgca tctcgccgcg ccaaccgggc gcgaatggcg
     1381 ggccagaggt cggcctcggg cacctcgcgc gcggccaggc ggcgcagctc gcgccggatg
     1441 cgctgctcat tcatcgggta cgctcctcgt cgtggtcggg tcgtcggtac cgagaaggtg
     1501 gcgcaggcga gcgcgcgccg cgtgcaggcg ccacttgatc gtgccgagcg ggctgcccag
     1561 ccgccgcgcc gtctcctgct ccgtcaggtc gaggtagtag cgcagcacga tggcggcgcg
     1621 ctgctcggcg ggcagttgat cgagcgcctg ctgcagcgag cgcgatgtct ccagcagctc
     1681 ggccagccgt tggggctcgg cctccaggcc gtcgagcgcc gcgcccccac cccaatcgtc
     1741 ttcaagcggc tgctgccggc gctcgcgccg cgcggctttc agcgcgtcgt tgaccacgcc
     1801 gcgcaggaac caggggccga agggccgagt cgcatcgaac tgcgcgatgc gctcgtaggc
     1861 ccggatgaag gcgccctgca cgatatcctc ggccagcgcg cggtcgcgcg tgatcagcgt
     1921 ggccgcgcgc acggcctgca gctggtagcg gcgcaccaac ggctccagcc cgccgatgtc
     1981 gccccggcgc agccgctcga tcgcctcccg ctcgctcccg ctgacgacca ccccagcacc
     2041 ccctctaggg atactatccg cgactcggcg gaatggttgg gccattttcg cgcttccagg
     2101 tagcacggcg cggcgtgcta tcgttcgcgc ggcggctcgg ccgggcgcgg ccagcggatg
     2161 actgggagag ggagtgcgtg atggttgagt tcggcgacat cctggcggcg cgagaagcca
     2221 tctccggtga agtgcgccag acaccggtct tcagcgccac gcgcatcggc cagcgcgccg
     2281 gtgtgcgcct gcagctcaag gccgaattgc tgcagaagac cggctccttc aaggcgcgcg
     2341 gcgcgctcaa cgccatcctc cagacgccgc gcgcgcagct cgagcgcggc gtgatcgccg
     2401 tctcggccgg caaccacgcg caggggttgg cctgggccgc gcgccaggcc ggcgtgcgct
     2461 gcgtgatcgt gatggcggcc agcgccagcc gcaccaagat cgccgccacc gaggggtatg
     2521 gcgccgaggt cgtgctggtc gaaggaggcg tgacggccgc cttcgagcgc gccgccgagc
     2581 tggagcgcgc cgaggggctg gtcaacatcc acggcttcga caacccgctg atcatcgccg
     2641 gccagggcac ggttgggctg gagatcatgg agcaggtacc gggtgtcgaa gtcatcgtct
     2701 gcccggtggg cggcggcggg ttgatcagtg gcgtggcgct ggcggccaag tcgctcaagc
     2761 cgagcgtgcg cgtctacggg gtcgagccgg agggcgcttc ggccatgcgc cagtcgtggg
     2821 accgcggcga accggtcacg ctctcctcgg tcaacaccat cgccgacggc ctggcgccgc
     2881 cgatggccgg ccagctcacc tacccgctga cgcggcaata tgtcgacgac atcgtcacgc
     2941 tgagcgacga ggagatcgtg gttggtctac gcgcggtcct gaccgaggcc aagctctacg
     3001 ccgagccctc gggcgccgcc gccaccgccg cgctgctggc cggcaagatc ccccacgagc
     3061 ccggcgcgct gaccgtcgcc atcgtcagcg gcggcaacat ggatctggag cggctgaaga
     3121 cgctgatctg agggcgcgcg gggagccctc atccccagcc cttctcccaa cgggttggga
     3181 gaagggagca cgatgggctg taggggcggg tttcgaacct cccccgcccg aatccgcacg
     3241 cgacaccaat cgtcctcgct agcaacccac ccgccctccg ccgctacccc cgcgcgtggt
     3301 ggatgacgta gaggtcgtag tcggcggtgt gcagttggtg ctcggcccag acaccgccgt
     3361 actggctctt cgtcagccgt ccgagcaatt gctgctcgtt gctgtggtag gccgcggtgt
     3421 cgctctggta gatcagcacc tcgtccaccc cgcgctgccg caggaaagcg gcgtactcgt
     3481 cggcgttgac gaagctgccg tggtggacgc tctcggggaa gaaggaggag tcgagccgcg
     3541 ccccgtgctg gatcagctcg tacatgccca ccttgtcgtc gccgacgcgc agaaagcgat
     3601 aggtctggcc cggctggaag gcgggcgagg cgatgaaggc cagcagagag gtgttcggcc
     3661 ggcgctccag cgctctggcg gcctgcgcgg tgtgcagcag cggcagcgtg gcgatgccca
     3721 gcgccagcac ggcggcacag gcggcgggcg ccagccgcgg gcggcggtgc tggagcgcgg
     3781 ccagcaagag cggcagcgcc acgaccggcg cgcgctcggc cagcgtgccg aagaaattgg
     3841 cggcgatggt gccgaaggaa ctctccagcg tcaccggtga ggccagcgcg aaccagacgg
     3901 ccggggccgc gagcagcagt gaggtgccgt aggccaccag cagacggtcc ggccgcggct
     3961 cccagcgcag ccaggcgagc accaagaggc cggccagcgg cagcacgatc ggcgcgtggg
     4021 tggcctgcgc caacccgccg aggatgatcg cccaccagaa gtgatctcgt cgccaggcgc
     4081 cgaccgccgc cagcagcagc gcgctggccc agaggaaggg aagctgaccc agcagcagac
     4141 tcgccagcag cgccgagttg agcagcaccg ccgcggccgg ccacacgcgc cgcagctcgg
     4201 gcagggccca gaagctggcc gccagcgtgc cgaccgcgcc cagcgccagc cagagcgtca
     4261 ccacccagtc gccgagcagc ggccgcagca gggccgcgct gagccagggc acaaagctgt
     4321 aggggaaagc gtaggcttcc ccgtgttcga gcagcggcat gtgcatcggg atgcgcccat
     4381 ggtgccagag ccgccggctg acgtaccaga cgtgcgcgta gttgttgagc gagtcgttgc
     4441 tgacgaagat gacgtgccgc agcagcagcg ccagcaccac cagccagccg gcgccggccg
     4501 ccagcagcac ggccagcccc agccgttgga gctcgcgcga ctccagcgcg gccagcagtg
     4561 ccgtcacccg ctgctgaggg tgtgggttgg gatagactac gcgcacaagc tctcccgctc
     4621 gtcgccgcac gcgaccagat ggaccggaat gcgcagatga taccgaaccg ctggcagcag
     4681 gtggatcgct ggatcggcgg cgcggcgctg gccgcgctgc tgctgccggg gctggcgctg
     4741 gcgcccggcc gcgggaacct cctgccgccc ctgctggcgg tcctggcggc cgtcgccggg
     4801 gcattcctgg ccaaccgctt caccccgcgc ccgcgcggcg gcctggccct gctgggcgcg
     4861 ctgatggtgg cgctggccgc ctggctggcg gcgcagatcc acttcgccct gaccgcctac
     4921 cta
//
LOCUS       DASUBI010000837         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1139323, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000837 DASUBI010000000
VERSION     DASUBI010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139323"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..520)
                     /gene="greA"
                     /locus_tag="VFU72_16540"
     CDS             complement(50..520)
                     /gene="greA"
                     /locus_tag="VFU72_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5425153.1"
                     /translation="MPAERAVVLTAEGKDELEHELQTLRSVKLPDVTRRVQELTLDGD
                     VSDNSEYEDTKEELMIVEARIREIEHLLRRAQVIDKTPNDGVVKLGSQVTLVDEDGHT
                     ERWTLVSPAEANTMNAKISTDSPVGAALLGKRAGEAVTVSAPGGDTTYRVQLVE"
     gene            complement(723..796)
                     /locus_tag="VFU72_16545"
     tRNA            complement(723..796)
                     /locus_tag="VFU72_16545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Val,seq:cac)
     gene            complement(851..1234)
                     /locus_tag="VFU72_16550"
     CDS             complement(851..1234)
                     /locus_tag="VFU72_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5425154.1"
                     /translation="MPKSVVATQQAPQAIGPYSQAIRLGNLLFTAGQIPLDPSSGKLV
                     EGDIEQQTERVMRNLQAILEAAGSSLDQVVKTTCFLADLDDFQRFNQIYGQFFTNQPP
                     ARSTVQVARLPAGARVEVECVAECG"
     gene            1458..2453
                     /locus_tag="VFU72_16555"
     CDS             1458..2453
                     /locus_tag="VFU72_16555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5425155.1"
                     /translation="MPQLSCSVVIASYNGWELLQPCLRALESQTRAPEQVLVVDNGSR
                     DGTSAELRRAFPAVEVLPLGGNLGFAAANNQGILRSESDVVVLLNNDTEAEPEFLARL
                     LAPLEADRSLGAVAATMVFAGAPSVVASAGIEVYRNGLALDRALGCERAALRDQEVFG
                     PSAGAAAYRRAALEDVGSFPETFFMYLEDVDLAWRLRLRGWRTWQASGAVVRHAYSAS
                     AVEGSDFKRRLLARNRVWLLARCLPRPLLARHGWRVLRYDALAFGYGLATRDWATAAG
                     RVVALGGLGRRVRERRAIQSGATAEWTEIERWLLPEPSAREMLRLRHLAARHAVG"
     gene            2550..>2831
                     /locus_tag="VFU72_16560"
     CDS             2550..>2831
                     /locus_tag="VFU72_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425156.1"
                     /translation="MAGRAVDDHTTTFWINTRPTPHVAITRAVLGAMAALATVVVLVA
                     GVLLVQGWRYRDRIDPGVRAGGIALAGLSQEQAATVLAARLPADAPRQLV"
BASE COUNT          402 a          927 c          988 g          514 t
ORIGIN      
        1 cggacgatgc accttgcccg cggccgctgc cctcaatcga ctcggacgat tactcgacca
       61 actgaacccg gtaggtcgtg tcaccgcctg gggcgctgac cgtgacggcc tcgcccgcac
      121 gtttgcctag caacgcggcg ccaaccggcg agtcggtgga aatcttggca ttcatggtgt
      181 tggcctcggc cgggctgacc agcgtccagc gctcggtgtg gccgtcttcg tcgaccaacg
      241 tgacctgcga gccgagcttg accacaccgt cattgggagt cttgtcgatg acctgggcgc
      301 gacgcagcag atgctcgatc tcacggatgc gcgcctccac gatcatcaac tcttctttgg
      361 tgtcttcata ctcgctgttg tccgacacgt cgccgtcgag cgtcagttct tgaacgcgcc
      421 gggtaacgtc tggcagcttg accgagcgca gcgtctgcag ctcgtgttcc agctcgtcct
      481 tgccttcggc cgtgagtacg accgcgcgct ctgcgggcat gatcaccctt cttccttgct
      541 caccgcgaac cgcgccagcc acctgagcca tgtggacgct ccgggataca ttgagaaccg
      601 aggacaccga tgccctcggc caactgcgct accggattgt cttcgagctc aaacagacta
      661 tgaccgcgga gccacatcac ccgcgccgtg cgtccgcgct cggcgttgct gccgggtggg
      721 ggtgggtgag aagggacttg aacccctgac ctcgcggatg tgaaccgcgc gctctaacca
      781 gctgagctac tcacccgctc gcgcccacag tatatcatcc aggctccagt ttgacgatga
      841 tccgggtcgc ctagccgcac tccgcgacgc actcaacttc gacgcgcgcg ccggccggta
      901 gccgtgcgac ctggaccgtg ctgcgggcag gtggctggtt ggtgaaaaac tggccgtaga
      961 tctggttgaa gcgctggaaa tcgtcgaggt cggccaggaa gcaggtcgtc ttgacgactt
     1021 gatcgagcga cgagccggcg gcttcgagga tcgcttgcag gtttcgcatc acgcgctcgg
     1081 tctgctgctc gatgtcgccc tcgaccagct tgccggacga gggatcgagc gggatttgcc
     1141 cggcggtgaa caggagattg ccgagccgga tcgcctggga atacgggcca atggcctgcg
     1201 gcgcctgctg cgtcgccacc acgctctttg gcatttggtc ccctctctgc cgtgaacaac
     1261 cggccagtcg cggccgtcgc ggcattctcg cacggatgcc gccgcgcgac aaaccggatg
     1321 tcggcggcac ccaggcttcg cggctggtat cctctatcct tcgggatata cgccgcgaca
     1381 gcgcgcggcg ccgcgacgct ccgtccgggc cgaagccgct gcgctccgtt ttgaagccgg
     1441 cccggtggag gcggttaatg ccgcagctca gttgttcggt ggtgatcgct tcatacaatg
     1501 gctgggagct actgcagccc tgtctgcggg cgctggagtc ccaaacgcgt gcgccagagc
     1561 aggtcttggt ggtcgacaac ggcagccggg atggcacatc tgctgagctc aggcgcgcgt
     1621 tcccggccgt agaggtgctt ccgctgggag gcaacctggg cttcgcggcg gccaacaacc
     1681 aggggatcct ccggagcgag agtgacgtgg tcgtgctgct gaataacgac acggaagccg
     1741 agccggagtt tctcgcccgg ctgttggcgc cgctggaggc ggaccgctcg ctgggcgcgg
     1801 tcgcggccac gatggtcttc gccggcgccc cgtccgtcgt cgcctcggcc gggatcgagg
     1861 tctaccgcaa cggactggcg ctcgaccgtg cgcttggctg cgagcgtgcg gcgctccgcg
     1921 atcaggaggt cttcgggccg tcggcgggtg cggcggccta ccggcgcgcg gcgctcgaag
     1981 acgtggggag cttcccggag acctttttca tgtatctgga ggatgtggat ctggcctggc
     2041 gcttgcggct gcgcggctgg cgcacctggc aggcgtccgg ggcggtggtg cgtcacgcct
     2101 actcggcctc cgcggtggaa ggctcggact tcaagcggcg cctgctggcg cgtaaccgcg
     2161 tctggctgct ggcgcgctgc ctgccacgcc cgctgctcgc gcgccacggc tggcgtgtct
     2221 tgcgctacga cgcgctggcc ttcggttacg gcctggccac gcgcgactgg gccaccgctg
     2281 ccggcagagt ggtggcgctg ggtgggctgg gccggcgcgt gcgcgagcgg cgggcgattc
     2341 agtccggcgc gacagccgag tggaccgaga tcgagcgttg gctgctgccc gagccgtcgg
     2401 cgcgtgagat gctccggctc cgccacctgg ccgcgcgtca tgcggtagga tagggtgcag
     2461 ggaaggttag gagcaacggg gactgccggt tgcggccgcg gtcgccgaca ctacgcacag
     2521 tggctccgaa gtgaagggtt ggaattcgcg tggccggccg agccgtcgac gaccacacca
     2581 ctacgttctg gatcaatacc cgtcccacac cacacgtggc gatcacgcga gccgtgctgg
     2641 gcgctatggc cgccctggcg acggttgtcg tgctggtggc gggcgtgctg ctggtacagg
     2701 gttggcgcta ccgcgaccgc atcgaccccg gtgttcgggc tgggggtatc gcgctcgccg
     2761 gtctcagcca ggagcaggcg gcgacggtgc tggcggcgcg gttgccggct gatgccccgc
     2821 gtcagctcgt g
//
LOCUS       DASUBI010000838         5586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_65050, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000838 DASUBI010000000
VERSION     DASUBI010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65050"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFU72_16565"
     CDS             complement(<1..965)
                     /locus_tag="VFU72_16565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5425157.1"
                     /translation="MAAETSQVHHGAPDQDVSAEMKQAFHQFSAKDFGKTLFHRFQKD
                     NVTGLASQVAYNLIYAIPPLIILLVTLAALLNATTGLAVTDTLHSAITNHAPSTAQPL
                     LNQLVTNAIENAGASAVSIGAVVAFLLALYGASGGVGSLIDAFNRAFEVEGQRGFVKS
                     KALVFGLTLLTSLLVIAAFVLPLIAHSLGSWVAGLVGLGSLFTLLWSVASWVLAAFFI
                     VLVLATLYYFGPDVKQSWKWISPGSVIATILWLLAIVGFKLYLAVSNPASGYGAAGGV
                     VVLLTFLWVTGLVFILGAEINAILEKRYDIKTIRDLAGHPAKTTP"
     gene            complement(1151..2176)
                     /gene="aroF"
                     /locus_tag="VFU72_16570"
     CDS             complement(1151..2176)
                     /gene="aroF"
                     /locus_tag="VFU72_16570"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5425158.1"
                     /translation="MIIRMQEDADDSATDAVIAELAGRGYETHLIRGGGAAAIGVNGK
                     PVSADLGCTLELAPGVDAVINGSTPYKLAARAARPQGTVVQVGSVAIGREAPVMIAGP
                     CAVESREQTLRTAEAVARGGAVILRGGAYKPRSSPYSFQGLGEEGLRILAEARALTGL
                     PVVTEVMEPGQVELVAEYADMLQIGSRNMMNVPLLRRVAASGKPVVLKRGFAATIEEW
                     LLAAEYILAGGNDRVVLCERGIRSFDSATRFTLDLNAVPLLRRLTHLPILVDPSHGTG
                     LRELVPAMSLAAVAAGAHGVMLEVHHAPEDALCDGPQSITAATLADIAQRAAALHALL
                     SPPLCVA"
     gene            complement(2487..2711)
                     /locus_tag="VFU72_16575"
     CDS             complement(2487..2711)
                     /locus_tag="VFU72_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425159.1"
                     /translation="MEEELAAQQREIGGHRIGICDLCGKPSDDLSLMSLDGDSRPGET
                     DQPINVCRDCAEQLTRGDFPLDPEDFEPEH"
     gene            2996..3469
                     /gene="greA"
                     /locus_tag="VFU72_16580"
     CDS             2996..3469
                     /gene="greA"
                     /locus_tag="VFU72_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5425160.1"
                     /translation="MQRERVPVTREGLEKLQEELEHLTTVRRPEVVRAIAQAREEGDL
                     RENAGYEAAKHDQGFIEARIRELEDLLRRVDVIEENEAPDNRRVRLGSTVTVEIDGVS
                     ETYTIVGKVEAKPGEGRISNESPVGRALLGKHQGDELTIETPGAPMRARVLKIAG"
     gene            3470..3955
                     /locus_tag="VFU72_16585"
     CDS             3470..3955
                     /locus_tag="VFU72_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5425161.1"
                     /translation="MVPEDGPARLGRYLRQQDVAAEIVFPGAPTPTVPNAAAALGVAP
                     AQIVKSLLFEGRDGDLVLAVLAGDQRVDRRKLIAVSGLRQPKLADPALVLARTGYPAG
                     GTPPVGHREPLRVLLDRALLAEPVVFGGGGSDRAMLRIAPAEIVRLTGATVADLGRED
                     A"
     gene            3955..5085
                     /locus_tag="VFU72_16590"
     CDS             3955..5085
                     /locus_tag="VFU72_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425162.1"
                     /translation="MVSATLLRDDVARVATSSLKVFDACLLPNGCLVAAPSHHPAYPA
                     RARGALFCWPGRELGFAITGMEALGQAVRQPLLSWLWDRADSFQETGLLFEDYHVNGI
                     RRGREWQPDQAGTLLWALCRAPDSWDRLEAAVVRKLATALVGLWDGNDFRARYRDIWQ
                     ERYADPAYGTHFPYSLAAAAAGLWLAAEVHGIQAWRVAAGQMAGRAADGWNARLGQFM
                     RRFGRSGNDSNVDASLLGLIWPFSVVDDADRLHAIVESVEARLLSEIGVFRYVYDSYD
                     GEVDAFGEDLRQGAGAWPLLTFWLAIAHSRLGDQARALQTFQIGLEAVDQDGFLPEQI
                     FPPGDARVGVKPRLWSHMLFALAAEELGLLARRPDGPARGQP"
     gene            complement(5287..>5586)
                     /locus_tag="VFU72_16595"
     CDS             complement(5287..>5586)
                     /locus_tag="VFU72_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425163.1"
                     /translation="EAHGAARKRAAGEASAIGEIVAQEIKSERRSEVPVVLVTTAAGA
                     HAYVNLNHVVGVVEMANEHVTRIYFSAGPIQFVDVQQTPDQIISQAGITSGNIQI"
BASE COUNT          864 a         1874 c         1974 g          874 t
ORIGIN      
        1 tccggggtgg tcttcgcggg gtggcctgcc aggtcgcgga tcgtcttgat atcgtagcgc
       61 ttctccaaaa tggcgttgat ctcggcgccg aggatgaaga ccaaaccggt gacccagaga
      121 aaggtcagca acacgacgac accgcccgcg gcgccgtagc cactggcggg gttggagacc
      181 gccaggtaga gcttgaagcc gacgatcgcg agcagccaga ggatggtcgc gatcaccgac
      241 cccggcgaga tccacttcca ggactgcttc acgtccggtc cgaagtagta cagggttgcc
      301 agcaccagca caatgaagaa cgcggccagc acccagctcg ccacactcca caggagggtg
      361 aagagcgagc cgagcccgac cagcccggcg acccaactac cgagactgtg ggcgatgagc
      421 ggcagtacga aggcggcgat caccagcagg ctggtgagca gcgtcagccc aaagacgagc
      481 gccttgctct tgacgaagcc gcgctggccc tcgacctcaa aggcgcggtt gaaggcgtcg
      541 atcaaggagc caactccgcc ggaggcgccg tagagcgcca gcaggaaggc gactacggct
      601 ccaatggaga cggcgctggc gccggcattc tcgatggcgt tggttaccag ttggttcagc
      661 agcggctgcg cggtgctcgg agcgtgattg gtgatggcgc tgtggagggt atcggtaacg
      721 gccaggccgg tcgtggcgtt cagcagcgcc gccagggtga ccagcaagat gatcagcggc
      781 gggatggcgt agatgagatt gtaggcgacc tgggaggcca gcccggtgac gttgtctttt
      841 tggaagcggt gaaagagggt cttgccgaag tccttcgcgg agaactgatg gaatgcctgc
      901 ttcatctccg cgctgacgtc ctgatcgggt gccccgtggt gcacctggct ggtttccgcc
      961 gccatctcac tcgtcctttc tccggctgcc caggcgcgcc gcgccgcggt cgttagtcag
     1021 ggcgtatgca accgacgtgc cagccggggc cgttcccacg cgccagggaa cgtcgccgac
     1081 ggacagcggc cgcaccccgt acgcgaatcc gcgcgtgagg ggccgcgagt ggcctgcccg
     1141 gccgccgcga tcaggccacg cagaggggcg gcgacaggag ggcgtggagg gcggcggcgc
     1201 gctgagcgat gtccgccagg gtggcggcgg tgatcgattg cgggccgtcg cagagcgcgt
     1261 cttcgggcgc gtggtggacc tctagcatca cgccgtgcgc tccggccgcg acggcggcga
     1321 gcgacatggc cggaaccagc tcgcgcaggc cggtgccgtg gctgggatcg accaggatcg
     1381 gcaggtgagt cagtcggcgc agcagcggca ccgcgttgag gtcgagcgtg aagcgcgtag
     1441 cgctgtcgaa gctgcggatg ccgcgctcgc agaggacgac ccggtcgttg ccgccggcca
     1501 gaatgtactc ggcggccagc agccactcct cgatggtggc ggcgaagccg cgcttgagca
     1561 cgacgggttt gccgctcgcg gccacccggc gcagcaatgg cacgttcatc atgttgcggc
     1621 tgccgatctg cagcatgtcg gcgtactccg ccaccagctc gacctggccc ggctccatca
     1681 cctcggtgac gaccgggagg ccggtcaacg cgcgcgcctc ggccaggatg cgcaagccct
     1741 cctcgcccaa cccctggaag ctgtacggcg acgagcgcgg tttgtaggcg ccgccgcgca
     1801 agatgaccgc gccgccccgc gctacggcct cggccgtgcg cagcgtctgc tcgcggctct
     1861 cgaccgcaca ggggccggcg atcatgaccg gcgcctcccg gccgatcgcc accgagccga
     1921 cctgcaccac ggtcccctgc gggcgggcgg cccgcgccgc cagcttgtag ggcgtgctgc
     1981 cgtttatcac ggcgtccacc ccaggcgcca gctccagagt acagcccagg tcggcgctta
     2041 ccggcttgcc gttgaccccg atcgccgccg cgccgccgcc gcggatcagg tgcgtctcgt
     2101 agccgcgccc ggccagttcg gcgatgaccg catcggtcgc gctgtcgtcg gcatcttcct
     2161 gcatccggat gatcacgccg cccctccttc ggatttggac aaacaaaaaa gcagaggatc
     2221 gttcctctgc ttcccccgac gttgctggct ggttaggctg ctaccggatc aacaccgatc
     2281 gccctgccag caacgccgga gggctaaagt accaaaaccg atacgagcgg tgttgttgca
     2341 atcctcaccc cgatttcatg ccgggtgcgt acacaacgcc actgtaatgc atccgccacg
     2401 ggctgtcaac gttggccgcg aaagatcctg ccgcgcgatc caggcgggcc gagaggtgga
     2461 gcggcccgcc cgtgcgcgca cgccgatcag tgttccggct cgaagtcttc aggatcgagc
     2521 gggaagtcgc cgcgtgtcag ttgctcggca cagtcgcggc agacgttgat tggctggtcc
     2581 gtctcgccgg gccggctgtc cccgtcgaga ctcatcaagc tcaagtcgtc gctcggtttg
     2641 ccgcacaggt cgcagatgcc gatgcgatgg ccaccgatct cgcgctgctg ggcggccagt
     2701 tcttcctcca tcgcgccctc cctccaatcg tgctgcctgt gaccccgagc accgggtcgt
     2761 gtccactccg gcgtcagagc ggctcgcgac cagtggtggg gccgtcttct cagcacgatg
     2821 cgggccacga gcatgcggcc aggggccggg gggaacgcgc cgtgcggagt cgcggttggc
     2881 gcgcggcggg gcgagcggta aagtgtaggg acgctcgctc acgacgacag ccggagcaat
     2941 gcgcggcggc gcggcggaca tcttcagcgg tatcgagtgt aaggaacgtg ctggtatgca
     3001 acgggaaagg gttcccgtca cccgcgaggg tttggagaaa ttgcaggagg agctggagca
     3061 tctgacgacg gtacgacggc cagaggtggt ccgtgccatc gctcaggcgc gcgaggaagg
     3121 tgacctgcgg gagaacgcgg gctatgaagc cgccaagcac gaccagggct tcatcgaggc
     3181 gcgcatccgc gagctggaag acctgctgcg acgagtcgac gtgatcgagg agaacgaggc
     3241 gcccgataac cggcgcgtcc ggctgggctc gaccgtcacg gtggagatcg acggggtttc
     3301 cgagacctac accatcgtcg gcaaggtcga ggcgaagccg ggcgagggcc gcatctcgaa
     3361 cgagtcgccg gtcggccgcg cgctgctcgg caagcaccag ggtgacgagc tgacgatcga
     3421 gacacccggc gcgccgatgc gagcccgggt actgaagatc gcgggctagg tggtgccgga
     3481 ggacggcccg gcgcggctag gccgctacct gcgccagcag gacgtggcgg cggagatcgt
     3541 cttccccggt gcgccgacgc ccaccgtgcc caacgcggct gccgcgctcg gcgtggcgcc
     3601 ggcccagatt gtcaagtcgc ttctcttcga agggcgcgac ggcgacctgg tgctggcggt
     3661 gctggcgggc gaccagcggg tggaccgtcg caagctgatc gcggtcagcg gcctgcgcca
     3721 gccgaagctg gccgatcccg cgctggtgct ggcgcgcacc ggctaccccg ccggcggcac
     3781 gccgccggtc gggcatcgcg agccgctccg ggtcctcctc gaccgcgcgc tgctggccga
     3841 gccggtcgtc ttcggtggcg gtggcagcga ccgggccatg ctgcgcatcg cgcccgccga
     3901 gatcgtccgg ctgaccggcg ctaccgtcgc cgacctcggc cgagaggacg cctgatggtc
     3961 agcgcgacgc tgctgcgcga cgacgtggcg cgcgtcgcga cgtcttccct gaaggtcttc
     4021 gatgcctgcc tgctgcccaa cggctgcctg gtagcggcgc ccagccacca tccggcctac
     4081 ccggcgcgag cgcgcggcgc actcttttgc tggcccgggc gcgagctggg tttcgccatc
     4141 accggcatgg aagcgttggg ccaggcggtg cgccagccgc tgctctcctg gctctgggat
     4201 cgcgccgaca gctttcagga gaccgggctc ctgttcgagg actaccacgt caacggcatc
     4261 cgccggggtc gcgagtggca gcccgatcag gccggcacgc tcctctgggc gctctgccgc
     4321 gcgccggact cctgggaccg gctagaggcg gcggtcgtcc gcaagctggc gacggcgctg
     4381 gtcgggctct gggacggtaa cgattttcgc gcccgctacc gcgatatctg gcaggagcgc
     4441 tatgccgacc cggcctacgg cacgcacttc ccctacagcc tggcggccgc cgcggccggg
     4501 ctctggctgg cggccgaggt ccatggcatc caggcctggc gggtggcggc ggggcagatg
     4561 gcggggcgag cggccgacgg ctggaacgcg cgcctgggcc agtttatgcg ccgctttggg
     4621 cgcagcggca acgactccaa cgtcgatgcc tcgctgctcg ggctgatctg gcccttcagc
     4681 gtggtcgacg acgccgaccg gctgcatgcg atcgtcgaga gcgtcgaggc gcggctgctc
     4741 agtgagatcg gcgtcttccg ctacgtctac gacagctacg acggcgaggt cgacgccttc
     4801 ggggaggacc tgcgtcaggg ggcgggcgcc tggccgctgc tgaccttctg gctggcgatc
     4861 gcgcacagcc gtctgggcga ccaggcgcgg gccctgcaga cgttccagat cgggttggaa
     4921 gctgtcgacc aggatgggtt cctgccggag cagatcttcc cgccgggcga tgcacgggtg
     4981 ggggtcaagc cgcgtctctg gagccatatg ctgttcgcgc tggcggccga ggagctcggt
     5041 ctactggcgc gccgacccga cggccccgcg cggggccagc cgtagtgacg ctgcccggcg
     5101 acatcgttgc gtgtcactcc agcacggatc cttcgactgc gctcaggatg acgtgaatgg
     5161 gggatggggg cgaacgacga ggcgtgtcct ccgcgtcagc ccccattcaa cccggtcatc
     5221 ccgaccgaag tggaaggatg cgtgccgcac acggcacgca tccttcattt ccgccgcaag
     5281 acgccgctag atctggatat tgccgctggt gatcccggcc tgggaaatga tctgatccgg
     5341 cgtctgctgc acgtccacga actgtatcgg accggcgcta aagtagatgc gcgtcacgtg
     5401 ctcgttcgcc atctcgacca caccgaccac atggttgagg ttgacatagg cgtgggcgcc
     5461 cgcggccgtc gtcaccagca ccaccggcac ctcgctgcgt cgctcgctct tgatctcttg
     5521 cgccacgatc tctcctatcg ctgaagcctc gcctgccgcg cgtttgcgcg cggcaccatg
     5581 tgcctc
//
LOCUS       DASUBI010000839         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1283626, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000839 DASUBI010000000
VERSION     DASUBI010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283626"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFU72_16600"
     CDS             <1..546
                     /locus_tag="VFU72_16600"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEU5425164.1"
                     /translation="IEHLHLALGPAGNVADSEIVRVIEGGPLRLHFRLETDGDWRARR
                     LRAEAPFPDGARLELQSDGAGHWVDGDGRALPALEGCLDVDIAATPFTNTLPVRRLGL
                     GEGQAAEISVVYFGIPDLRPRPERQRYTWLRATPDGAVYRYEGLETGFRVDLAVDGDG
                     IVLDYPGVWRRAWPPLAPINT"
     gene            574..1398
                     /gene="menB"
                     /locus_tag="VFU72_16605"
     CDS             574..1398
                     /gene="menB"
                     /locus_tag="VFU72_16605"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEU5425165.1"
                     /translation="MTVNWVPLGEYEDILYEQAEGEGIAQITINRPEVHNAFRPETVA
                     EMIDAFADVRERLDIGVVLLRGAGTRAFCSGGDQRKREAGGYAGTDRVPRLNVLDLQR
                     QIRHLPVPVIAVVAGYAVGGGHVLHVICDLTIAADNAIFGQAGPRVGSFDGGYGAGYL
                     ARIVGHKRAREIWYLCRQYSAEEAFQMGLVNTVVPLERLEDEAIQWSREILVKSPTAI
                     RLLKAAFNADTDGLAGIQQLAGDATLLFYQTPEAQEGSKAFVEKRKPDFTKYPRFP"
     gene            1534..2445
                     /locus_tag="VFU72_16610"
     CDS             1534..2445
                     /locus_tag="VFU72_16610"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEU5425166.1"
                     /translation="MEQGVGVTAPRPSAWRAWVHAARPPTLPAAVVPVLVGTAAGVRE
                     DSFHLLPFLGAFVAALLIQIGTNFANDLSDFHKGADTESRVGPTRITASGIATPAQVK
                     KATILTFGLALVIGLYLVAVGGWPILVIGLASILAGLAYTGGPWPLGYHGLGDVFVFI
                     FFGVLAVSGSAYLQTGRLSTLALAASIPVGLLVTNILVINNLRDLATDRAAGKNTLAV
                     RLGPNPTRVQYALFSVVAYVVPALLIITHLAPPWALLPWLTLPLAARLTGTVLGGMTG
                     RSLNRVLQLTGQLHLLFGILFALGLLL"
BASE COUNT          375 a          863 c          930 g          417 t
ORIGIN      
        1 atcgagcacc tgcacctggc gctcgggccg gctgggaacg tggctgacag cgagatcgtg
       61 cgcgtgatcg agggcgggcc gctgcggctg cacttccggc tggagactga cggagactgg
      121 cgagcccgga ggctgcgggc cgaggcgccc tttcccgacg gcgcgcggct cgagcttcag
      181 tccgacggcg ccgggcattg ggtcgatggc gatggccggg cgctcccagc gctggagggc
      241 tgcctcgacg tcgacatcgc ggccacgccc ttcaccaaca ccttgccggt gcgccggctg
      301 gggctgggcg aggggcaagc ggccgagatc agcgtggtct acttcggtat cccggatctg
      361 cgcccgcggc cggagcggca gcgctacacc tggctgcgcg ccacacccga cggcgcggtc
      421 taccgctacg agggcctgga gaccggcttc agggtcgacc tggcggtcga tggcgacggg
      481 atcgtgctcg actatccggg agtctggcgc cgcgcgtggc cgccgctggc gccgatcaat
      541 acgtgagtgt cgtatggtca cgagggaggt tcgatgactg tcaactgggt gccgctaggc
      601 gagtacgagg atatcctcta cgagcaggcc gagggtgagg ggatcgcgca gatcacgatc
      661 aaccggccgg aggtccacaa cgcctttcgt ccggagaccg tggccgagat gatcgacgcc
      721 ttcgccgacg tgcgcgagcg gctggatatc ggcgtggtgc tgctgcgcgg cgcgggcacg
      781 cgcgccttct gctccggcgg cgaccagcgc aagcgcgagg cgggcggcta cgccggcacg
      841 gaccgcgtgc cgcggctcaa cgtgctcgac ctgcagcggc agattcgcca tctgccggtc
      901 ccggtcatcg cggtggtggc cggttacgcg gtgggcggcg gccatgtgct gcacgtcatc
      961 tgcgacctga cgatcgcggc cgacaacgcc atcttcggcc aggccggtcc gcgcgtcggc
     1021 agcttcgacg gcggctacgg ggccggctac ctggcgcgca tcgtgggaca caagcgggcg
     1081 cgcgagatct ggtacctttg ccgccaatac agcgccgagg aagccttcca aatgggactg
     1141 gtgaatacgg tggtgccgct ggagcggctg gaggacgagg cgatccagtg gtcgcgcgag
     1201 atcctggtca agagcccgac cgcgatccgg ctgctcaagg cggccttcaa cgccgatacc
     1261 gacgggctgg ccggcattca gcagttggcc ggcgacgcca cgctcctctt ctaccagacg
     1321 cccgaggcgc aggagggctc gaaagccttc gtggagaagc gcaagcccga cttcacgaag
     1381 tacccgcgct ttccgtagac ttgccctcgc accggggcct cgcccccgac ggggagccct
     1441 catccccagc ccttctccca acgcgttggg agaagggagc acgacgagct gtaggggcgg
     1501 gcttcaaccc gcccaactga tgaaggattg gtgatggagc agggggtggg ggtgacggcg
     1561 ccgcggccga gcgcctggcg agcctgggtg cacgccgcac ggccgccgac gctgccggcg
     1621 gcggtggtgc cggtgctggt gggcacggcc gccggcgtgc gtgaggactc gttccacctg
     1681 cttccctttc tcggggcgtt cgtcgccgcg ctgctgatcc agatcgggac caatttcgcc
     1741 aacgacctgt ccgacttcca caagggcgcc gacacggaat cgcgagtcgg gccgacccgt
     1801 attacggcga gcggcatcgc cacgccggcg caggtcaaaa aggcgacgat cttgaccttc
     1861 ggactggcgc tcgtgattgg cctctatctg gtggcggtcg gcggctggcc gatcctggtc
     1921 atcggcctgg cctcgatcct ggcgggtttg gcctacaccg gcggcccctg gccgctcggc
     1981 taccacgggc tcggcgatgt cttcgtcttc atcttcttcg gtgtcctggc ggtcagcggc
     2041 agcgcctatc tccagacggg gagactcagc acgctggcgc tggcggcctc gatcccggtc
     2101 ggtctgttgg tgaccaacat cctggtgatc aacaacctgc gcgacttagc caccgaccgc
     2161 gcggcgggca agaacacgct ggcggtgcgc ctggggccga acccgacgcg ggtgcagtac
     2221 gccctcttct cggtggtggc ctatgtcgtg ccggcgctgc tcatcatcac gcatctggcg
     2281 ccgccctggg cgctcctgcc ctggctgacg ctgccgctgg cggcgcggct gaccggcacg
     2341 gtgctcggcg gcatgaccgg ccgctcgctc aaccgggtgc tgcagctcac cggccagctc
     2401 cacctcctct tcggcattct cttcgcgctg gggctgctgc tgtgaggggg cgagcggcgc
     2461 tgcccatcga acacgccgga agctgtcgag gacggatcct tcgacaagct caggatgacc
     2521 ggggaaggga tggggctgac gaggaggacg tgtctcgccc gattggcctc caaccaaaca
     2581 ctgtc
//
LOCUS       DASUBI010000840         5259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_407397, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000840 DASUBI010000000
VERSION     DASUBI010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407397"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..810
                     /locus_tag="VFU72_16615"
     CDS             73..810
                     /locus_tag="VFU72_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5425167.1"
                     /translation="MGTRILVLSDVHGNLVALEKVLEAAGPVDAIWSLGDIVGYGPRP
                     RECVDRIRDICPDVSLIGNHDWACIGRLSLEDFNPIARFASYWTTMQLTAEHLQYLES
                     LPNRMIDQDWTLVHGSPRHPVWEYVYNARIAALNFPLLDTRICFLGHTHVQMYIREED
                     ALANLPPRQPIEGQVLDLSQGRFMLNPGAVGQPRDGDPRAAFAIFEPEALRVTFRRVA
                     YDVAETQAQMRAEGLPESLASRLKLGI"
     gene            921..1370
                     /locus_tag="VFU72_16620"
     CDS             921..1370
                     /locus_tag="VFU72_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5425168.1"
                     /translation="MYRRIVVPVSGDWRDRFAINLSRAVAKLHGTDLTLVYVVEVPRA
                     LPLDADLPRDIEHGEQVLSSAADYARSVGDGSWLHVVTELLQARSAAAAVVDEAIDRE
                     ADAIVLAASNRLEHGVLTQGETVPYVFKNAPCDVLVVRARSEDEDQA"
     gene            1367..1786
                     /locus_tag="VFU72_16625"
     CDS             1367..1786
                     /locus_tag="VFU72_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5425169.1"
                     /translation="MKILIVGCGRLGGKLAGDFAGHGHDVTVIDMNPRSFRHLPGDYQ
                     GRTVLGTGIDSDVLRSAGIEEAEVFLAVTEEDNTNIMAAQLAQTTFQVPKVRLRIYDP
                     VRADVFRRLGLNVICPTTIVAGMLEEQVLGLVPQARS"
     gene            1806..2471
                     /locus_tag="VFU72_16630"
     CDS             1806..2471
                     /locus_tag="VFU72_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5425170.1"
                     /translation="MYIVVVGGGKVGYYLTKTLLSEGYEVFLIEKNPVKCATYADRFG
                     AVVMQGDGAEAGALADAGVARADVVIAVTGDDEDNLVISQLAKERFGVRRVIARVNNP
                     KNEELFRKLGIDVTVSQTNVILSLIEQQIPNLPFVHLMNLRHSDLAIVDAKVSAESPV
                     VDQPIERIQFPPEVIVSAIMRQGAVIVPSGQTVIKAGDQLICVAKREREEALRDLLAI
                     YES"
     gene            complement(2627..3904)
                     /locus_tag="VFU72_16635"
     CDS             complement(2627..3904)
                     /locus_tag="VFU72_16635"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5425171.1"
                     /translation="MAIESPTQGREQQNLFDIAVEQFHIAADVIGLDDDMRKLLSTCK
                     RELTVHFPVEMDDGTIQVFTGHRVQHNLGPGPAKGGIRYHQDVTIDEVKALAMWMTWK
                     CAVVGLPYGGGKGGVQVNPKLLSQSELQNLTRRYTTEISVIIGPHRDIPAPDVNTNPQ
                     IMAWILDTYSMHNGGVMIPSVVTGKPLLLGGSEGRFEATGRGCVFAIEEASKAYNINL
                     PNAHVVVQGFGNAGSTCARILYDLGCTIVGVSDSHGGIYNPKGLDIPAVMEHKLRNQS
                     VVGFPESQNVSNQELLELDCEILIPAALEEQITEDNAPRIKARLIAEAANGPTTPEAD
                     RILHDRGVIVLPDIFANAGGVTVSYFEWVQGIQAFPWTEEEVNTKLKRMMVDAFAAIH
                     ATAEQYHVHMRTAALARAIMRVAEFSRVRGIYP"
     gene            complement(4028..>5259)
                     /gene="gatB"
                     /locus_tag="VFU72_16640"
     CDS             complement(4028..>5259)
                     /gene="gatB"
                     /locus_tag="VFU72_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5425172.1"
                     /translation="KNYIYPDLMKGYQISQYDLPLCVEGHLDFVVEGAPQRVGIRRVH
                     LEEDTARLVHRTVAGESFSLVDVNRSGVPLMEIVTDPDLRSPEDARVFLIGLRQILRY
                     IGVSTGNMEEGAFRVDANISQRTIDGSVVGAKVEIKNMNSFRAVERALAYEVERQREI
                     LSGGGTIEQETRGWDDARGVTLGQRSKEYAHDYRYFPEPDLPPLTMVEAEVREIASRM
                     PELPRARAERFATEYALAPAEAALLTEERAVADYYEAAVAGERRYARPAATWISGELF
                     ALMRERGQGIEAINVGPGQLRRLIDLVEQGTINLRTAKDVLLAVAQTGAEPDAIVRER
                     GLGQVSDVARLEQVIREVIDHNPDAVADYQGGKQAAAGFLIGQVMKQLRGTANPSVAR
                     QLLTNMLNHAQSETDAD"
BASE COUNT          826 a         1762 c         1767 g          904 t
ORIGIN      
        1 gggtcacgcc gggagacgtg gcggttctga tggtgcatct ggagcgcctg agcgcggaag
       61 tagggtagag cggtgggaac gcgcatcctg gtcctgtccg atgtccacgg caatctggtg
      121 gcgctggaga aggtgctgga ggccgcgggt ccggtcgacg ccatctggtc gttgggcgac
      181 atcgtcggct acgggccgcg cccgcgcgag tgcgtcgacc gcatccgcga catctgcccc
      241 gatgtcagcc tgatcggcaa ccacgactgg gcctgtatcg ggcggctctc gctggaggat
      301 ttcaacccga tcgcccgctt cgccagctac tggacgacga tgcaactgac ggccgagcac
      361 ctgcagtatc tcgagtcgct tcccaaccgc atgatcgatc aggactggac gctggttcac
      421 ggcagccccc ggcacccggt ctgggagtac gtctacaacg cccgcatcgc cgcgctcaac
      481 ttcccgctgc tcgacacccg catctgcttc ctcggccaca cccacgtgca gatgtacatc
      541 cgcgaagagg atgcgctggc caacctgccg ccgcgccagc cgatcgaagg acaggtgctc
      601 gacctgtcgc agggccgctt catgctcaac cccggcgccg tcggccagcc gcgcgacggc
      661 gacccccgcg ccgccttcgc catcttcgag cccgaggcgc tgcgggtaac cttccgccgc
      721 gtcgcctacg acgtcgcgga gacccaggcg cagatgcgcg ccgagggact ccccgaatcg
      781 ctcgccagcc gcctcaagct cggcatttga gcgccgttgc cgccactcgc gcgtgggcgc
      841 atactttgcg cgctgcgctt ggcgcgacgc ggccagacgg cgaggtgccg ccggtggacg
      901 ccctacgtaa ggatgcattg gtgtatcgac gcatcgtggt ccccgtctcc ggcgactggc
      961 gcgaccggtt cgcgatcaac ctctcacggg ccgtggccaa gctgcatggg accgacttga
     1021 cgctggtcta cgtggtggag gtgccgcgcg cgctgccgct cgacgccgac ctgccgcgcg
     1081 acatcgagca cggcgagcag gtgctgagca gcgcggcgga ctacgcccgc agtgtcggcg
     1141 acggttcctg gctgcacgtg gtgaccgagt tgctccaggc gcgctcggcc gccgccgccg
     1201 tggtcgacga ggcgatcgac cgcgaggccg acgccatcgt gctggcggcc agcaaccggc
     1261 tcgaacacgg cgtgttgacc cagggtgaga cggttcccta cgtgttcaag aacgccccgt
     1321 gcgacgtgct ggtcgtgcgc gcgcggtcgg aggatgaaga ccaggcatga agattctcat
     1381 cgtcggctgc ggccggctgg gcggcaagct ggccggcgac tttgccggcc acgggcacga
     1441 cgtcaccgtg atcgacatga acccgcgctc cttccggcat ctgccggggg attaccaggg
     1501 gcgcaccgtg ctcggcacgg gcatcgacag tgacgtgctg cgctcggccg gcatcgagga
     1561 ggccgaggtc ttcctggccg tcaccgagga agacaacacc aacatcatgg ccgcccaact
     1621 ggcccagacg accttccagg tgccgaaggt gcgcctgcgc atctacgacc cggtgcgcgc
     1681 cgacgtcttc cggcgcctgg ggctgaacgt catctgcccc accacgatcg tcgcggggat
     1741 gctggaggag caggtgctgg ggctggttcc gcaggcgcgg tcctagaggc tatgggagcg
     1801 taggtatgta catcgtggtc gttggcggcg gcaaagtcgg gtactacctg accaagaccc
     1861 tgctcagcga ggggtacgag gtcttcctga tcgagaagaa cccggtcaag tgcgccacct
     1921 acgccgaccg cttcggcgcg gtggtgatgc agggggacgg cgccgaggcc ggggcgctgg
     1981 ccgacgccgg cgtggcccgc gccgacgtcg tgatcgcggt caccggcgac gatgaggaca
     2041 acctggtgat ctctcagctc gccaaggagc gcttcggcgt gcgccgcgtg atcgcccgcg
     2101 tgaataaccc caagaatgag gagctctttc gcaagctggg gatcgacgtg accgtcagcc
     2161 agaccaacgt catcctcagc ctgatcgagc agcagatccc caatctcccc ttcgtccacc
     2221 tgatgaacct gcgccactcg gacctggcga tcgtcgacgc caaggtctcg gccgagtcgc
     2281 cggtggtgga tcagccgatc gagcgcatcc agttcccgcc ggaggtgatc gtctcggcca
     2341 tcatgcgcca gggcgcggtg atcgtgccgt ccgggcagac ggtgatcaag gccggcgacc
     2401 agctgatctg cgtggccaag cgcgagcgcg aagaagccct gcgcgacctg ctggcgatct
     2461 acgagagctg agggctgccc tcaccccctg cccctctccc atgcgtgggc gaggggaggt
     2521 ggctatagac gagggttagg cgttagggat gcttccctat cgtgctgcct gctcgaaggg
     2581 cagcgcccct ctcccgcgcg cgggagaggg gtcgggggtg agggccctag gggtagatcc
     2641 cacgcacgcg gctgaactcg gccacgcgca tgatggcgcg cgccagggcc gcggtgcgca
     2701 tgtggacgtg gtactgctcg gcggtggcgt ggatggcggc gaaggcgtcg accatcatcc
     2761 gcttcagctt ggtgttgacc tcctcctcgg tccaggggaa ggcctgaatc ccctgtaccc
     2821 actcgaagta ggagaccgtc acgccgccgg cgttggcgaa gatatcgggc aggacgataa
     2881 cgccgcgatc gtgcaggatg cggtcggcct cgggcgtagt cggcccattg gccgcctcgg
     2941 cgatcaggcg cgccttgatg cgcggcgcgt tgtcctcggt gatctgctcc tcgagcgccg
     3001 ccggaatcag gatctcgcag tccagctcca gcagttcctg gttgctgacg ttctgcgact
     3061 cggggaagcc gacgaccgac tggttgcgca gcttgtgctc catcacggcc gggatgtcga
     3121 gccccttggg attgtagatg ccgccgtgcg agtcgctcac gccgacgatg gtgcagccca
     3181 ggtcatagag gatgcgggcg caggtcgagc cggcgttgcc gaagccctgc accacgacgt
     3241 gcgcgttggg aaggttgatg ttatacgcct tggacgcctc ctcgatggcg aagacgcagc
     3301 cgcggccggt cgcctcgaag cgtccctcgg acccgcccaa gagcagcggc ttgccggtca
     3361 ctaccgaggg gatcatgacg ccaccattgt gcatgctgta ggtatcgagg atccaggcca
     3421 tgatctgcgg gttcgtgttg acgtcgggcg ccgggatatc ccggtgcgga ccgatgatga
     3481 ccgagatctc ggtggtatag cgccgggtca ggttctgcag ctcgctctgg gaaagaagct
     3541 tcgggttgac ctgcacgccg cccttgccgc cgccgtacgg caggccgacc acggcgcatt
     3601 tccaggtcat ccacatcgct agcgccttga cctcgtcgat ggtcacgtcc tggtgatagc
     3661 gaataccgcc cttggcaggg ccggggccga ggttgtgctg cacccggtgg ccggtgaaga
     3721 cctggatcgt gccatcgtcc atctccaccg gaaagtgaac cgtgagctcg cgtttgcagg
     3781 tgctcagcag cttgcgcatg tcgtcatcga ggccgatcac atcggcggcg atgtggaact
     3841 gctcgaccgc gatgtcgaag agattttgct gctcccggcc ctgagtcggg ctctcaattg
     3901 ccattcatgg atcccttcta taccatggcc ggtcacccca ttgtgccggt ctccccgcgt
     3961 ggctggaaaa cgccgtgcac ctcctcgcca aaaaatatgg gagggtgctt gcgcccctcc
     4021 cactctacta atccgcgtcc gtctcgctct gcgcgtgatt gagcatgttg gtcaacagtt
     4081 gcctcgcgac cgacgggttg gctgtcccgc gcagctgttt catcacctgg ccaatcaaga
     4141 agccggcggc ggcctgcttg ccgccctgat agtcggcgac cgcgtcgggg ttgtgatcga
     4201 tgacctcgcg gatgacctgc tccagccgcg ccacgtcgct gacctggcct agcccgcgct
     4261 cgcgcacgat cgcgtccggt tcggcgccgg tctgcgcgac cgccagcagc acgtccttgg
     4321 ccgtgcgcag gttgatcgtg ccctgctcga ccagatcgat gagccggcgc aactgcccgg
     4381 ggccgacgtt gatcgcctcg atcccctgtc cgcgctcgcg catcaacgcg aaaagctcgc
     4441 cggagatcca ggtggccgcc gggcgcgcgt agcgccgctc gccggccacg gcagcctcat
     4501 agtagtcggc caccgcgcgc tcctcggtca gcagcgccgc ctcggccggc gccagcgcgt
     4561 actccgtcgc gaagcgctcg gcgcgggcgc gtggcagctc cggcatacgg gaggcgatct
     4621 cacgcacttc ggcctcgacc atcgtcagtg ggggcagatc cggctccggg aagtagcggt
     4681 agtcgtgcgc gtattctttc gagcgctgcc ccagtgtcac cccacgggcg tcgtcccagc
     4741 cgcgcgtctc ctgctcgatc gtcccgccgc cggagaggat ttcgcgttgg cgctcgacct
     4801 cgtaggccag cgcgcgctcg accgcgcgga agctgttcat attcttgatc tcgaccttgg
     4861 cccccaccac ggagccgtcg atggtacgct gcgagatgtt cgcgtcgacg cgaaaggcgc
     4921 cttcctccat gttgccggtg gagacgccga tatagcgcag gatctggcgc aagccaatca
     4981 ggaagacgcg cgcatcctcg ggcgagcgca ggtcgggatc ggtgacgatc tccatcagcg
     5041 gcacgccgga gcggttgacg tcgaccaggc tgaagctctc accggccacc gtgcgatgta
     5101 ccaggcgcgc ggtatcctcc tccaggtgaa cccggcggat accgacgcgc tgcggcgcgc
     5161 cctcgaccac gaagtcgaga tgcccctcga cacagagcgg cagatcgtac tgcgagatct
     5221 ggtagccctt catcaggtcg ggatagatat agttcttgc
//
LOCUS       DASUBI010000841         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1302413, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000841 DASUBI010000000
VERSION     DASUBI010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302413"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFU72_16645"
     CDS             complement(<1..854)
                     /locus_tag="VFU72_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5425173.1"
                     /translation="MREPAADFVIETDRLTKHYGAVVAVEELSLRVPAGGVFGLLGPN
                     GSGKTTTIGMLLGLVRPTGGTIHLFGHPQGGVTEADLHRIGAIVETPTFYPYLSGRAN
                     LRFFQAIGSHDGAGELDSLLALVELAGAADRPYRTYSLGMKQRLGIAYALLGNPDLVF
                     LDEPTNGLDPAGVVEVRDLIRRLGSDGRTVILSSHLLGEVQQVCDQVAILSHGHLLVS
                     GKVSDLLQQQGGLRLTTTDNARAHDLLAALPFVHGVRPSGEQLIVSAPPERAWEITRA
                     LAEAGVYVA"
     gene            complement(929..1132)
                     /locus_tag="VFU72_16650"
     CDS             complement(929..1132)
                     /locus_tag="VFU72_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425174.1"
                     /translation="MNAIARFLRFWYDFVVGDDWTVLVGVLIALVVTALLAHSGVAAW
                     WLMPLAVALVLGASLWRVTRPAG"
     gene            complement(1134..1907)
                     /locus_tag="VFU72_16655"
     CDS             complement(1134..1907)
                     /locus_tag="VFU72_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425175.1"
                     /translation="MSALFLVLAAFLASAVEMVEALTIVLAVGVTRGWRSALRGVAVA
                     LVALAVVVAVLGPALTHIPLNGLRLVVGTLLVVFGLQWLRKAILRASGYKALHDEDAI
                     FQRELAEAREASSVIRAGMDWYAFTLSFKGTFLEGLEVVFIVLTFGSTQGNIPLAAAG
                     AFAAIVLVGLAGALVHRPLSRVPENTMKFAVGVLLTTFGTFWSAEGAGVTWPGSDAAI
                     FGVLAFVLVVSLTFVALLRRQHELRLAPVHSGMSAAEED"
     gene            complement(1904..2560)
                     /locus_tag="VFU72_16660"
     CDS             complement(1904..2560)
                     /locus_tag="VFU72_16660"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEU5425176.1"
                     /translation="MSDLAWLLLVYRVPSEPASKRVGIWRELKRLGALYLQQCVCIVP
                     RRDELAKELEQVVARITALEGEATLFDVPRLRPADEERILAAFRELRDKEYAEIVEEC
                     ATKFVKEIEFEHFRQNYSYAEAEEIAQDLDKIRRWFERVVTRDWFGGTRRAEVEGWIE
                     HCQALLDGFEHAVYQRQGDDGGPHLPVLPLAPLPGWQPARPGSDDDIEEDSESKEARS
                     "
BASE COUNT          681 a         1254 c         1271 g          543 t
ORIGIN      
        1 gcgacgtaca cgccggcctc cgccagcgca cgggttatct cccaggcacg ctccggcggc
       61 gcgctgacga tcaactgctc accgctgggc cggaccccgt gcacgaacgg cagcgcggcc
      121 agcagatcgt gcgcgcgcgc attgtcggtc gtcgtcagcc gcaagccacc ttgctgctga
      181 agtagatccg agactttgcc gctgaccagc agatggccgt gcgagaggat ggccacctgg
      241 tcgcagactt gctgcacttc ccccaacagg tggctggaga gtatcacggt gcgcccgtcg
      301 gagccgaggc gacgaatgag atcgcggacc tcgaccacgc ccgccgggtc cagcccgttg
      361 gtcggctcgt cgaggaagac cagatcggga ttaccgagca gcgcataggc gataccgagg
      421 cgctgcttca tcccgagtga gtaggttcgg tagggacgat cggctgcgcc ggcgagctcg
      481 accagcgcca ggagcgaatc gagctcgccg gcgccgtcgt ggctgccgat agcctgaaag
      541 aagcgcagat tggcgcggcc ggagaggtag gggtagaagg ttggcgtctc cacgatggcg
      601 ccgatgcggt gcaggtcggc ctcggtcacg ccgccctggg gatggccaaa gaggtgaatc
      661 gtgccgccgg tcgggcgcac caagccaagg agcatcccga tggttgtggt cttgcccgat
      721 ccgttcgggc caagcaggcc gaagacccct cccgccggca cgcgcaacga cagttcctcg
      781 accgcgacca ccgcgccata gtgtttggtc aagcgatcgg tttcgatcac gaaatcggct
      841 gcgggctccc gcacacgacg ctccgctcgc tcaagttgct tccgaaccta acaacaccgc
      901 tgggggcgga actgtgacgc ggccggcgct agccggccgg gcgcgtgaca cgccagagcg
      961 aggcgccgag gacgagggcc acggcgagcg gcatcagcca ccaggcggcg acgccggagt
     1021 gcgccagcag cgcggtgacg accagcgcga tcagcacccc gaccagcacc gtccagtcgt
     1081 ccccgacgac gaagtcgtac cagaagcgca ggaagcgcgc gatcgcgttc atctcagtcc
     1141 tcctccgcgg cgctcatgcc tgaatgcacc ggcgccaggc gcaactcgtg ctggcgccgc
     1201 agcagcgcca cgaacgtcag cgatacgacc agcacgaagg caagcacgcc gaagatggcg
     1261 gcatcgctgc cgggccaggt aaccccggcg ccctcggcgc tccagaaggt gccgaacgtt
     1321 gtcaagagca cgccgacggc gaatttcatg gtgttctccg gcacgcggct gagcgggcga
     1381 tgaaccagcg cgccggccag cccgacgagc acgatcgcgg cgaacgcgcc ggcggcagcc
     1441 aaggggatgt tgccctgcgt gctgccaaag gtcagcacga tgaagacgac ctcaagccct
     1501 tcgaggaagg tgcccttgaa ggagagcgta aacgcgtacc agtccatgcc ggcacggatg
     1561 acgctggacg cctcgcgcgc ttcggcgagc tcgcgctgga agatggcgtc ctcgtcgtgc
     1621 agcgccttat agccgctggc gcgcaggatc gccttgcgca gccactggag gccgaagacg
     1681 accaacagcg tgccgacgac caggcggaga ccgttgagcg ggatgtgcgt gagcgccggt
     1741 ccgagcacgg ccaccaccac cgccagcgcg accagcgcga ccgcgacgcc cctgagcgcg
     1801 gagcgccacc cgcgcgtgac gccgaccgcc agcacgatgg tcagcgcttc gaccatttcc
     1861 acggccgagg ccagaaaggc ggcgaggaca agaaagagcg cactcacgag cgggcctcct
     1921 tcgattccga gtcctcttcg atatcgtcat cgctgccggg ccgcgccggc tgccagccgg
     1981 gcagcggcgc cagcggcagg acggggagat gcggaccgcc gtcgtcgccc tggcgctggt
     2041 agacggcgtg ctcgaagcca tcgagcagcg cctggcagtg ctcgatccag ccttcgactt
     2101 cagcgcgccg cgtgccgccg aaccaatcgc gcgtcaccac gcgttcgaac cagcgccgaa
     2161 tcttgtccag gtcctgcgcg atctcctccg cctcagcgta gctgtaattc tggcgaaagt
     2221 gctcgaactc gatctccttg acgaacttgg tggcgcactc ctcgacgatc tcggcgtact
     2281 ctttgtcgcg cagctcgcgg aatgccgcca gaatgcgctc ctcgtcggcc ggtcgtaggc
     2341 gcggaacatc gaagagggtc gcctcgccct ccagcgcggt gatgcgcgcg acgacctgct
     2401 ccagctcctt cgccagctcg tcgcgcctgg gcacgataca gacgcattgt tgcaggtaga
     2461 gcgcgcccag gcgtttcagc tcgcgccaga tgccgacgcg cttgctggcc ggttcactcg
     2521 ggacgcggta gaccaagagt agccaggcaa gatcggacac gtacaccccc ccgtcgttac
     2581 agatgcaacc atgttacacc tgtacatcgg ccatggcaat gctcgtgctg gcgcggcctg
     2641 ctcctccgga tgcacgcgag aatgtgccgc atccgggggc tggattggcg attgagcagg
     2701 gctccagggt cagcttgggc ggcgcgaccg gatgcccggc cgcccgggcg ggtttggctt
     2761 gaccggggtg tgcgatgcgg acgtcgccgc gccggtcgtg tcgcatgcgg cggaacgcgg
     2821 gagggtattc caaaagacag acgaggctca acagcagaac cagcaagagt tgccgaggcg
     2881 gggggatgcg agcgcgaaac gtggttccca gcccaacgac acagagcacg gcgacggcgc
     2941 ttaggccgcc gagatccaga tggaaggcag tcatccgtcg ctccctcggc cgagacacta
     3001 cacgcgcgct gcggccgttc cctgtccctg tccgaatccg ccaggcgggc gcggactcgc
     3061 cggctgccgt ggctggagtc atgaatagaa cgtttgaagg acgcaaacgg cttcagttcg
     3121 ccgtggcatc gcactccgtg ggcaggtgga tcgatggttc gagtcggcga gcacacccgg
     3181 gtggacgcgg tggccaccac ctgccgcgac cgtgacgact gggggactca ccgcgttaac
     3241 ggtgcggcgg atcatacgct cgcgttcccg ccggtcgaga tttgggttga cgcatagccg
     3301 ctcgggaaca gacaggttgg actggatata cgccgctcgg tatactctcg gcctcaactt
     3361 cctgtcgtgt tgatcgcacg ccgcgcgccg atcacactcc ggacccggcg acctgcgcgt
     3421 ctatcgagcg gatgagggca acgagcagcg cggacctcag gcggccagtt ggccgccgcc
     3481 gcccgcacga cggcgatggc cgtcgaaagg aggaccagag ggggagagcg aaggggcaag
     3541 gaaacgttcc cggcgcattc gtgactcccg tggcagcgcg acgaaacccc gccgcgggac
     3601 catgtatgga attgagacga gcaccgatga cagtgcgcac caaaccggcg cggagggtag
     3661 ctccctgcgg cctcctcgcg ctgctgatta ttctcagtct gctggtgacc gcctgtgggg
     3721 gcagtgcggc caagacgacg ccaaccacg
//
LOCUS       DASUBI010000842         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_294050, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000842 DASUBI010000000
VERSION     DASUBI010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294050"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFU72_16665"
     CDS             complement(<1..297)
                     /locus_tag="VFU72_16665"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5425177.1"
                     /translation="MHITIGMDPNILSLGPFVIAWHALAILVAIVIAVQIVKWEFRRK
                     GLSFAHWDAMVYWTVAGGIIGARLFYVIDHLGYHLHHPLEIFALNQGGLAIYGSV"
     gene            complement(552..1226)
                     /locus_tag="VFU72_16670"
     CDS             complement(552..1226)
                     /locus_tag="VFU72_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425178.1"
                     /translation="MLYQFVSGDFPITLTILAVLAYWAMRHRSTVQELWRDERPVWTW
                     LGRAAIVLAVLIPLWVAAFDNWRDLLGYSLSAHDRWMSNPFKTTPASEMVRWVTFGLI
                     GASVLVDALLYARRRNGLWLLVIMIALGGSYFYFMNSIRIRVDALLLQAEDSLRHPEA
                     LGLAFTLFWALGLYVFIVSTLFAIYLTFFGVAAFVISLIYSLLNRGERIEPSTLTILH
                     GLRKTE"
     gene            complement(1355..1702)
                     /locus_tag="VFU72_16675"
     CDS             complement(1355..1702)
                     /locus_tag="VFU72_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU5425179.1"
                     /translation="MAADCLFCKIAAGNAPARIVYADDAVTAFHDIRPQAGTHLLVIP
                     NRHLDSLDAAGEEDAELLARLLQVAARVAREAGIAESGYRVVTNTGAHGCQSVPHLHF
                     HVLGGNQLSPVLG"
     gene            1736..2563
                     /gene="mutM"
                     /locus_tag="VFU72_16680"
     CDS             1736..2563
                     /gene="mutM"
                     /locus_tag="VFU72_16680"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5425180.1"
                     /translation="MPELPEVETVRRSLAEAIRGATISDLWVGPFSGSLAGITPEAMR
                     AEIVGEQIVKIGRRAKYLLLTLSNERTLAIHLRMTGELTIARPEEPSGKHHHLTFVLA
                     DGRELRFSDIRKFGRIRLLDAAELAVLDAALGPEPLAPDLTPEMFHARLQEHRRAIKP
                     LLLEQHFLAGVGNIYADEALFAAGIHPLRPASSLTVEESDRLLQALRDILSGAIARRG
                     TTLRNYRDGLGQSGGNQFHLRIYNRAEGEPCPRCGSPVRRLVVGQRGTKFCPRCQPL"
     gene            complement(2564..3799)
                     /locus_tag="VFU72_16685"
     CDS             complement(2564..3799)
                     /locus_tag="VFU72_16685"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5425181.1"
                     /translation="MYSYITNLECTYCGETFDKGKLMTTCPKCGKVLFARYDLKAAAK
                     QMTKEALKDRPWNLWRYHEILPVENPDDALTLGEAGTALLPTPRLGQALGLGNLLVKD
                     DGQNPTGSFKARGLCAAVSMARELGAKSVAIPSAGNAASAMSAYAARGGLDAFVFMPA
                     DAPEVMKAECVSYGAKVFLVKGLINDAGKIVREGSADQGWFDVSTLKEPYRAEGKKTM
                     GLELAEQLGWRVPDAIVYPTGGGTGIVGMWKAFDELEQMGLIGSKRPKMIVVQSTGCA
                     PIVKAFQEGKRHAELWQGAETLAPGIRVPVAIGDYLILDAVRQSGGTAIAVPDSEIMQ
                     GVRLLAAEEGVFASPEVGAVVMAAKHLREDGFLKPDDEVLLYATGSGMKHTDLVHGEY
                     PLLDPNAADVVAQVVRLAS"
     gene            3913..3985
                     /locus_tag="VFU72_16690"
     tRNA            3913..3985
                     /locus_tag="VFU72_16690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3946..3948,aa:Gly,seq:ccc)
     gene            complement(4124..>4694)
                     /locus_tag="VFU72_16695"
     CDS             complement(4124..>4694)
                     /locus_tag="VFU72_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5425182.1"
                     /translation="ARLWESGPYYGRPYHEIAAPFDTVYVSFYKGLGGLTGSALAGPA
                     DVIAEARVWQHRQGGRLFQLFPYVLSARHGLEQRLGRMADYHRRAVEIASDLAAFPQI
                     AIVPNPPQTNMMHLYLPGDRDRLLAAAVEIAEESGLGVLRALAPTPLPGYHKWEFTVG
                     DATLALSREEITATIGALLERAGSYARPD"
BASE COUNT          787 a         1563 c         1588 g          756 t
ORIGIN      
        1 gaccgagccg tagatcgcca gcccgccttg attgagggcg aagatctcca gcgggtgatg
       61 caggtggtag ccaagatggt cgatgacata aaacaggcgg gcgccgatga tgccgccagc
      121 caccgtccag tagaccatgg cgtcccagtg cgcgaacgac agccccttgc gccggaactc
      181 ccatttgacg atctgcaccg cgatgacgat ggcgacgagg atcgcgagcg cgtgccaggc
      241 gatgacgaac ggccccaacg agaggatgtt cgggtccatg ccaatggtga tgtgcaaagc
      301 gttctctcct ccggtcggtc ctatcggggc caattgtgcc acgcggggga tgcgggggga
      361 ggggtggcca gcgacgacgt tgcgtggcgt acgaggacgg atccttcgac tgcgctgagg
      421 atgacaggga caagggagag cctgacgacg acgactcgct gtattcgtca gcccagatct
      481 aaccctgtca tcccgaccga agtggaggga tccgtcctgg ggcgccccat aacggtgtac
      541 gtgacccgcg cctactccgt cttgcgcagc ccgtgcagga tggtgagcgt gctcggctca
      601 atgcgctcgc cgcgattgag caggctgtag atcaggctga tcacgaaggc cgccacaccg
      661 aagaaggtca ggtagatggc gaagagggtc gagacgatga agacgtagag cccgagcgcc
      721 cagaagagcg tgaaggccag cccgagggcc tccgggtggc gcaggctgtc ctcggcctgc
      781 aagagcagcg cgtcgacccg gatgcggatc gagttcatga agtagaagta cgagccgccg
      841 agcgcgatca tgatcaccag cagccagagg ccgttgcggc ggcgcgcgta gaggagcgcg
      901 tcgaccagta ccgaggcgcc gatcaggccg aaggtgaccc agcgcaccat ctcggaggcc
      961 ggcgtcgtct tgaacgggtt gctcatccag cggtcgtgcg cgctgaggct gtagcccagc
     1021 agatcgcgcc agttgtcgaa cgcggccacc cacaggggga tcagcacggc cagcacgata
     1081 gccgcccgcc cgagccaggt ccaaaccggc cgctcgtcgc gccacagctc ctggacggtg
     1141 gagcgatgcc gcatcgccca gtaggcgagg accgccagga tcgtcagcgt gatcgggaag
     1201 tcgcccgata cgaattggta gagcatgaca tcctcagccg ttcaggcttc cgtggcgcgg
     1261 cacagcgcct aacctggccc aattgtatcg ttcagggcag gcgaagagga tcgcagagta
     1321 tcgatacaag agggaatcgg cggccgctcc cgggtcagcc caggactggg ctcagctgat
     1381 tgccgccgag cacgtggaag tgaaggtgcg gcaccgactg gcagccgtga gcgccggtgt
     1441 tggtcaccac gcggtagccg ctctcggcga tgccagcctc gcgcgccacg cgcgcggcta
     1501 cctggaggag ccgcgccagc agctccgcgt cttcctcgcc ggccgcgtcg agcgagtcga
     1561 ggtgccggtt ggggatcacc agcagatgcg tgccggcctg gggccggatg tcgtgaaagg
     1621 cggtcacggc gtcgtcggcg tagacgatgc gcgcgggtgc atttccggcc gcgatcttgc
     1681 agaataggca gtcagccgcc atcgtcgttg tcagcccttt ctaccgggag tggtcgtgcc
     1741 ggagctgccc gaagtcgaga ccgtgcgccg tagtctagca gaggcgatca ggggcgccac
     1801 cataagcgac ctgtgggtcg gcccgttcag cggcagcctg gccggcatca cgccggaggc
     1861 catgcgcgcc gagatcgtgg gcgagcagat cgtcaagatc gggcggcgcg ccaagtattt
     1921 gctgctgacg ctctccaacg agcgtaccct cgccatccac ctgcgtatga ccggcgagct
     1981 gaccattgcc cggcccgagg agccgagcgg caagcaccac cacctgacct tcgtgctggc
     2041 cgacgggcgc gagctgcgct tcagcgacat ccgcaagttc ggccgcatcc ggctgttgga
     2101 tgccgccgag ttggccgtgc tcgacgccgc gctcggtccc gagccgctgg ctcccgactt
     2161 gacgccggag atgttccacg cccggctcca ggagcaccgg cgggcgatca agccgctgct
     2221 gctggagcag cactttctgg ccggtgtcgg caacatctac gccgacgagg cgctcttcgc
     2281 cgccggtatc cacccactgc ggccggccag ctcgctcacg gtggaggaat ccgaccggct
     2341 gctgcaggcg ctgcgcgata tcttgtccgg cgccatcgcg cggcgcggca cgacgctgcg
     2401 taactaccgc gacggcctcg gacagtccgg cggcaaccag ttccacctgc ggatctacaa
     2461 ccgcgccgag ggcgagccgt gcccgcgctg cggctcgccc gtgcggcggc tggttgtcgg
     2521 ccagcgtggc accaagttct gcccccgctg ccagccgctg tagctacgac gccagcctga
     2581 ccacctgcgc caccacatcg gcggcgttgg ggtcgagcag cgggtactct ccgtgaacca
     2641 ggtcggtgtg cttcatgccc gagccggtcg cgtagagcag cacctcgtcg tccggcttga
     2701 ggaagccatc ctcgcgcagg tgcttggcgg ccatcaccac cgcgccaacc tccggcgagg
     2761 cgaacacgcc ctcctcggcg gccagcaggc gcacgccctg catgatctcg ctgtcgggca
     2821 cggcgatggc cgtgccgccg ctctggcgta ccgcgtcgag gatcaggtag tcgccgatgg
     2881 cgaccggtac gcggatgccc ggtgccagcg tctcggcacc ctgccacagc tcggcgtggc
     2941 gcttaccctc ctggaaggcc ttgacgatcg gcgcgcagcc ggtcgactgc accacgatca
     3001 tcttggggcg cttggagccg atcagaccca tctgctccag ttcgtcgaag gccttccaca
     3061 tgccgacgat gccggtgccg ccgccggtcg ggtagacgat ggcgtcgggc acgcgccaac
     3121 caagctgctc ggctagctcc agccccatcg tcttcttgcc ctcggcgcga tacggctcct
     3181 tgagggtcga gacgtcgaac cagccctggt cggccgagcc ctcgcgcacg atcttaccgg
     3241 cgtcgttgat cagccccttg accaggaaga ccttggcgcc gtacgagacg cactcggcct
     3301 tcattacctc gggcgcgtcg gcgggcatga agacgaaggc gtcgaggccg ccgcgggcgg
     3361 cgtaggccga catggccgag gcggcgttgc cggcggaggg gatggcgacc gatttggcgc
     3421 ccaattccct ggccatcgag accgcggcgc agagcccgcg cgccttgaag ctgccggtcg
     3481 ggttctggcc gtcgtccttg accagcagat tgccgagtcc aagcgcttgg cccaggcgcg
     3541 gggtcggcag gagcgccgta cccgcctcgc cgagcgtcag ggcgtcgtcg ggattctcga
     3601 ctggtagaat ctcgtggtag cgccagaggt tccagggccg gtccttgagc gcctccttgg
     3661 tcatctgttt ggcggcggcc ttgagatcgt agcgggcaaa gaggaccttg ccgcacttgg
     3721 ggcaggtcgt catcagcttg cccttgtcga aggtctcgcc gcagtaggtg cactcaaggt
     3781 tcgtgatgta gctgtacatt ccctctcctg acccgtaacg taacccgttt ctgccggccg
     3841 cgcacacggc tcgcattgcg attgtaacgc tggacgccag atgctcaacc tgctatactg
     3901 ccgcgtgaca ctgcgggtgt aactcagttg gtagagtgtc tgcttcccaa gcagaatgtc
     3961 gtgggttcga gtcccatcac ccgctccccg catttctgcg atccccgacc gccaactcgg
     4021 tctgtctgtg tctgcatacg agcgatccgg acacgagaac cggcgcgata gcagtccgtt
     4081 cctcacggtc gatcccctgt caaggctcag cgcaccgtgg gaatcaatcc ggacgcgcgt
     4141 agctcccggc ccgctccagc agcgcgccga tcgtcgcggt gatctcctcg cggctgagcg
     4201 ccagcgtggc gtcgccgacg gtgaactccc acttgtgata gccgggcagt ggcgtcggtg
     4261 ccagcgcgcg caggacgcca agaccgctct cctccgcgat ctcaaccgcg gcggccagca
     4321 ggcggtcgcg gtcgcctggg agatagaggt gcatcatgtt cgtctgcggc gggttgggca
     4381 cgatcgcgat ctggggaaac gccgccagat cggacgcgat ctcgaccgcc cggcggtgat
     4441 agtcggccat ccgtcccagc cgctgttcga gaccgtgccg cgccgacagc acgtagggaa
     4501 agagctggaa gagccggccg ccctgccggt gctgccagac gcgcgcctcg gcgatcacgt
     4561 ccgctggacc cgcgagcgcc gatccggtca gaccgccgag ccccttatag aacgagacgt
     4621 agaccgtatc gaacggcgcg gcgatctcgt ggtagggccg cccgtagtat ggaccggact
     4681 cccacagccg ggcg
//
LOCUS       DASUBI010000843         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1024316, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000843 DASUBI010000000
VERSION     DASUBI010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024316"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFU72_16700"
     CDS             <1..380
                     /locus_tag="VFU72_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5425183.1"
                     /translation="GIAARVTRQYSRMGALLDPIIDRALVIAGVVVCWKFELLPRWML
                     DVMVAREAFMLLAGQYALRHGVTLKINWFGRWAVWPVMSALMMGLVALKTAGTVALAI
                     GLVLAVLATVQYGRDAITELRRR"
     gene            complement(358..909)
                     /locus_tag="VFU72_16705"
     CDS             complement(358..909)
                     /locus_tag="VFU72_16705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5425184.1"
                     /translation="MLPPLSLLLLFSPLLLLVVIPLCVWVHELAHAAMVLRLTGGRAV
                     VVVGRLPAACVISLRRLEIRWHPSLRAAFCEWDARDVTVRTARRIIMAGPLANAVQSV
                     LFLWLTIATWAAVPFWLWVELLTTFAVSGLICAVNLRSRPVNVLGKLTSTDGHKLRAL
                     RGLPADAVLTDGLRAVLSAGAAR"
     gene            complement(1187..2330)
                     /locus_tag="VFU72_16710"
                     /pseudo
     CDS             complement(1187..2330)
                     /locus_tag="VFU72_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          370 a          809 c          851 g          405 t
ORIGIN      
        1 acggcatcgc ggcccgggtg acgcgccagt acagccggat gggcgccctg ctggatccga
       61 tcatcgaccg cgccctggtg atcgccggcg tcgtcgtgtg ctggaagttc gagctgctgc
      121 cgcgctggat gctcgatgtc atggtcgcgc gcgaggcgtt catgctgctc gcgggccagt
      181 atgcgctgcg ccacggcgtc accctgaaga tcaactggtt cggccgctgg gcggtctggc
      241 cggtgatgag cgcgctgatg atgggcctgg tggcgctgaa gacggccggg accgtggcgc
      301 tggcgatcgg ccttgtcctc gcggtgctcg ccacggtcca gtacgggcgc gatgcgatca
      361 ccgagctgcg ccggcgctga gcacggcgcg gagcccatcg gttaggaccg cgtcggccgg
      421 caggccacgc agcgcgcgca gcttgtgacc atcggtggag gtcagcttgc caagcacgtt
      481 gaccggtcgc gaacggagat tgaccgcgca gatcaggcca gagaccgcaa aggtcgtgag
      541 caactcgacc cagagccaga acggtacggc ggcccaggtc gcgatcgtca gccacaggaa
      601 caagaccgac tgcacggcgt tggccagagg gccggccatg atgatgcggc gtgctgttcg
      661 gacggtcaca tcacgagcgt cccactcgca gaacgcggcc ctgaggctgg ggtgccagcg
      721 gatttcgagg cgccgcagcg agatcacgca cgccgcaggc agccgcccga ccaccacgac
      781 cgctcgcccg cccgtgaggc ggagcaccat cgcggcgtgg gccagctcgt ggacccagac
      841 gcacagcggg atgacgacca gcagcagcag agggctgaag aggagcagca aactcagcgg
      901 cggcagcatc cacaggcgtg atcggttctg ccggcggcaa gcttgagctc aagcccggga
      961 gtgagggcgg gctttcgctt tgcgggcgaa gcgtggtgag ctcgcagccc gccgtcgggt
     1021 ctgggccccc aaatggtcac tattcgaccg tcgcggggcc caggtccatg ccttgcgtgc
     1081 ggagcggcat ttcgggtcat cgagctaggg ggtgagccgc tgcgctggtt gcgtcaagtc
     1141 gcagttcgtg gtgtagctcg tgctcgtcgg tggtgacggt cgcggctcag gcggcgttga
     1201 gctcgatcac ctcctccttg tcttgctggt gggggtggtc gtctcggtct gtcgcggcgc
     1261 agaggacgag ccgcatgctt ttctcggaga ggtagcgccg gccgacaagc cactcatcgt
     1321 tctgttcgat cagcagggcg ccggcgaggc ggatcgcggc ggcgtcgttg gggaagatcc
     1381 cgacgacgtc agagcgccgg ccgatctcgc ggttgacgcg ctccagcggg ttcgtgctgc
     1441 gcagcttggt ccagtgcgcg cagggaaacg cgtagaaggc gatcaggtcc tcctcggcgt
     1501 cctccagcag ccgggcgacc ttcggcgcga tcggctccaa gcgcgcgatc acggccgtga
     1561 cgcgctcgcg tgcctgcgtg gcgtcggcgg cgttgaagac ctcgcgcagg gccgcggaga
     1621 cgaggccgcg ctgcgcgggc cggcagtgct gatgcatgtt gcgcacgaaa tggacggtgc
     1681 agcgctgcca cggcgcgttg atcgtccttg cgatcgcggt cttcagcccc tcgtggtggt
     1741 ctgagatcac gagcctgacg ccctccaggc cgcgggcttt caaggagcgc aggaactcaa
     1801 gccagaacgc ttcggactcg atctcgccga ggtccaggcc gatgatctcg cggctgcctg
     1861 tctcgtgcac ggcgtaggcg atcaccgccg ccttggaccg cacgtggccc tggtcgcgga
     1921 ccttcagctg cttggcgtcc agccacaagt acgggtaggc accctccaag gggcgctggc
     1981 gaaatagctc gacctgctcg tccagcgcct tgcacaggcg gctgacgcgg tccttgtcca
     2041 tcccgtggat cccgagctgc tcgaccagcc gatcgacctt cctggtcgag acgccgttga
     2101 cgttcgatct ccccgacccg ggtctcccac gccctgggcc gatagccgtt gcgatgcgtc
     2161 gtgcgctgcg ggctgcgctg cccgtgctcg gcgccgacct ccgaagagat ctcggcgtcc
     2221 atcacctccc ggcagaccaa cgcgatcgca tcgcgcaaca ggtcgccatg ctcgcctccc
     2281 atcaactcgg cgacgacatc agcagcggtc atcatctggc cctcggccat cgtggtgctc
     2341 cttctcgtct gactcctggt agagccgacg agcttgagcg ccgcggtggt cggacccttc
     2401 ttcaggccga ccccacgacg ccagctacac cactt
//
LOCUS       DASUBI010000844         8471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1089209, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000844 DASUBI010000000
VERSION     DASUBI010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089209"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFU72_16715"
     CDS             <1..210
                     /locus_tag="VFU72_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425185.1"
                     /translation="SWQSLFLTGSRALAPVGAGILYTLNGGYGPALWALTIGSALGTV
                     AILFADHRPEPATAEAATVARESAA"
     gene            258..1367
                     /locus_tag="VFU72_16720"
     CDS             258..1367
                     /locus_tag="VFU72_16720"
                     /inference="COORDINATES: protein motif:HMM:NF040505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HEU5425186.1"
                     /translation="MGTIDVAVIGGGQAGLATGYYLRRHGLSFAILDAEGGPGGAWRH
                     GWDSLALFSPAPWSSLPGWPLPGGAEHVPDRAAVLDYLAAYERRYHLPVHRPARVRAV
                     RRDGAALTVETDADVWWARAVVSATGTWRRPLWPWYPGQGEFAGQQLHSAQYRSPKVF
                     AGQRVLVVGGGNSGAQILAEVSRVAETTWVTRRPPRFLPDDVDGRVLFDRATARYHAL
                     VTGAAVPAADGGGLGQIVMTPAVREARERGVLQSAGPIARFTAAGVVWPDGRETPADA
                     VIWCTGFRPALGHLAPLGVVGAGGRVAVAGTRSVAEPRLWLVGYGEWTGFASATLIGV
                     GRSARATAEEIAASLAGTPQRPAAPSPAAIVGATQ"
     gene            1364..2593
                     /locus_tag="VFU72_16725"
     CDS             1364..2593
                     /locus_tag="VFU72_16725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5425187.1"
                     /translation="MRTSTLVAPRRFYYGWALVGTLAATETVSWDIVYYAFAVFLVPM
                     EAELGWSRAVLTGAYSLAIAGLAAIPVGRWLDRHGPRALMTADSIAAVLLLLAWSAVG
                     SVGAFYLIWAALGLVMAAILYEPAFAAVATWFERERGKALLLLTFVAGFASTIFLPLA
                     SRLVGALGWRQALLALAGILAVLTIPAHALVLRRRPEDLGLLPDGQERRGALGDAAFW
                     WLSGSIILGTVTVVALGVHLVPLLRERGYGADTAALATGALGALSVTGRVLITALIRR
                     LPQALVTAALLALQSIAVLVLLAWDSPAGLVLFVILFGLGFGALTLARAGLIAEWYGR
                     KHYGAIGGALALCTTGARALAPVGAGALYVWAGGYGQVLICVAVVAALAALAMIGAQF
                     TARPLATRTSEEHPCGG"
     gene            2936..4312
                     /locus_tag="VFU72_16730"
     CDS             2936..4312
                     /locus_tag="VFU72_16730"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU5425188.1"
                     /translation="MITTDEIHQQARQLNITPAAVQRDYVLGWLIFGVFARSALGRHL
                     ILKGGNALRKAYFANTRFSDDLDFTTQENLDPETLARELDLVCQFVQEHTGVAFDLEQ
                     NRVEEGRFITKEKRVYKVRLYFRGFAEHFEHLTLRVNVDITQNDRLSLPAQMRALIHP
                     YSDGDDCRTELRCVQLEEVLADKLKCLLQRRRSHDLYDLVHALFINTKVAIDRKAIIE
                     TFLGKTIFKPSPPALRDLLLEVPFEVFRDYWRKAIACPEGSHLPFDSAVDHFRQEIPA
                     LFAPFSYGEHASVAFFPARLRNPILEAGSGWKVLRVEYDGVTRLVEPYSLVFKRRMDG
                     HGQEYLYVYDRTGGRSSGPDIKSFVHSKLTRVEVTDEQFQPRFEVELSKAGEFGDRSY
                     FGRPFGGGTRAAGQVRRALSFSGMTYRIRCPFCNKTFRRKTMFDARLNAHKNEYGFPC
                     SGTIGFPD"
     gene            4414..4770
                     /locus_tag="VFU72_16735"
     CDS             4414..4770
                     /locus_tag="VFU72_16735"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5425189.1"
                     /translation="MITTLRRTNDPSAPPSFAVLARQLKALADPTRLAIVALLAKREQ
                     CVCDLTATLRLPQSTCSHHLGVLKRAGLIRDRQDGRDGRWSYYAIVPEAAEALHGHVG
                     NLLDLSGFDPTPAHCD"
     gene            4848..5534
                     /locus_tag="VFU72_16740"
     CDS             4848..5534
                     /locus_tag="VFU72_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEU5425190.1"
                     /translation="MSATPSQRAAAEAVGTFGLVFAGCGAIMIDMVSGGAITHVGVAL
                     TFGLIITTMIYATGHISGAHFNPAVTLAFAATRHFPLALVPVYLAAQFGGAVAASLAL
                     RLLFGDVAHLGATLPAGGAGQSLVLEVVLTFFLMFVIISVATDTRAVGQAAALAIGGT
                     VGLEAMFAGPICGASMNPARSLAPALVSGTWTAQWLYVVGPIVGALLGALMYQGLRGH
                     SGDAETLVGG"
     gene            5539..5997
                     /locus_tag="VFU72_16745"
     CDS             5539..5997
                     /locus_tag="VFU72_16745"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU5425191.1"
                     /translation="MGREWVLFLCTHNSARSQMAEGLLRHLAGDRFVALSAGTVATAV
                     RPQAIEVMREIGIDIARHESKTLERYLGEPLDLVITVCDDANEACPIFPGSHERLHWS
                     FEDPSRATGSPEEQLVVYRRVRDEIRARMERELLGRPPSERSERRVLGQS"
     gene            complement(6138..>8471)
                     /locus_tag="VFU72_16750"
     CDS             complement(6138..>8471)
                     /locus_tag="VFU72_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5425192.1"
                     /translation="DLVGGKGANLGRLVRLGFPVPPGFVVTTAAYRVAVTAGQLAATD
                     PEELRTRLPALPLPTEVSTPLLGAYRRLGATAVAVRSSGTAEDLAAASFAGQHDTFLN
                     VMGEAGLLDAVRACWASLWSPRAVDYRRRHGWAIPHLALAVVVQVMVPAEWAGVLFTA
                     DPVSGRRDRVVLEAVPGLGEALVSGEATGHRYVVTKRGQRVLSGDAALPRHLAADLAR
                     LGQRIEAAFARPQDIEWAYANGRVVVLQARPLTALPSETTPVAARRYSRFQRAGAPNA
                     LDHLPRPPYPLDVTLYFRPLLTRVLESLRGLGLVPPPVGDVLIEVAPGVVQMVPPTVR
                     PTLRALTLPAKLVAALRAATDPWLAAVRATLVARAEELDAEDLAPLADAAILARIEEL
                     QGLQLRHFMHRFGFLVPGLLVGLALPSLLRLAVGKRAADRLHADLLAAVPCITTEANR
                     ALGQLARQVRAAADVRAAFLTTPPDELPDRLHATAGGAAVRADIEAFLRRYGWRETAM
                     AGAGFPAWRDEPAVVYGLLRGLVVGEQAATALDDDPERVAGARRELDAALDRRRFLRP
                     LVVRAVAAARRSVAFREDSHFYLFLPFTVLRRLALELGQRLVARGVLAADSDIFFLEL
                     AELRPLNATDDLRERVLQRQAARRAVEDHYTAVPADLVAQTVQDGALVGASASPGRAV
                     GSVRIILREGDFWRLQPGEVLVAPYTNPAWTPLFAVASAVVVEAGGAASHAAIVAREY
                     GIPAVMGASNATRWLREGQRVLVDGERGRVTPLGDGT"
BASE COUNT         1223 a         2861 c         3051 g         1336 t
ORIGIN      
        1 agctggcagt cgctcttcct gaccggctcg cgggcgctcg cgccggtcgg ggcgggcatc
       61 ctctatacgc tgaatggcgg ctacggcccg gcgctctggg cgctcacgat cggttcggcg
      121 ctcggcacgg tggcgatcct ctttgcggac caccggcccg agccggcgac agcagaggcg
      181 gcgaccgtgg cacgggaaag cgccgcctga attcgcggtg cagggcgccg cgcgacgcgg
      241 catgaggggt atgcggcatg gggaccatcg acgtggcggt gatcggcggg gggcaggccg
      301 ggctggcgac cggttactac ctgcgccgtc acggcctttc ttttgccatc ctcgacgccg
      361 agggtgggcc gggcggggcc tggcggcatg gctgggactc gctcgcgctc ttctcccccg
      421 cgccgtggag ctcgttgccc ggctggccgc tgcccggggg cgcggagcat gtccccgacc
      481 gggcggcggt gctcgactac ctcgcggcct acgagcggcg ctatcacctc cccgtgcatc
      541 gcccggcccg cgtgcgcgcg gtgcgccgcg acggcgcggc ccttaccgtc gagaccgatg
      601 ccgacgtctg gtgggcacgc gccgtggtga gcgccaccgg tacctggcgg cgtccgctct
      661 ggccctggta ccccggccag ggcgagttcg ccggccagca actccactcg gcgcagtacc
      721 gttcgccaaa ggtgttcgcc gggcagcgcg tcctggtggt gggcggcggc aactccgggg
      781 cgcagatcct ggccgaagtg tcgcgcgtgg cggagaccac ctgggtcacc cggcgaccac
      841 cgcgcttcct ccccgacgac gtggatggcc gcgtcctctt cgaccgcgcc accgcgcgct
      901 accacgcact ggtgacgggc gcagccgtcc cggctgctga tggcggcggg cttggacaga
      961 tcgtgatgac gccggcggtg cgggaggcgc gcgagcgcgg cgtgctgcag agcgctggcc
     1021 ccatcgcgcg cttcaccgcg gccggggtcg tctggcccga cgggcgcgag acgcccgccg
     1081 acgcggtgat ctggtgtacc ggcttccgcc ccgcgctcgg ccatctggca ccgctcggcg
     1141 tggtcggcgc gggcgggcgc gtcgccgtgg cgggcacgcg ctcggtcgcg gagccgcgcc
     1201 tctggctggt cggctacggc gagtggaccg gcttcgcctc ggccacgctg atcggggtcg
     1261 gccggagcgc ccgcgcgacg gcggaggaga tcgccgccag cctggcgggg acgccccagc
     1321 gcccggccgc cccgagcccc gctgcgatcg tcggggcgac ccagtgagga cgagcacgct
     1381 cgtcgcgccg cgccgcttct actacggctg ggcgctcgtt ggcacgctgg cggccaccga
     1441 gaccgtctcc tgggacatcg tctactacgc cttcgcggtc ttcttggtgc cgatggaggc
     1501 ggaactaggc tggtcgcgcg ccgtgctcac cggggcctac tcgctggcga tcgcgggcct
     1561 cgcggcgatc ccggtcgggc gctggctcga ccgccacggc ccgcgcgccc tgatgacggc
     1621 cgactccatc gcggcggtcc tcctgctgct ggcctggtcg gcggtgggga gtgtcggggc
     1681 cttctacctc atctgggcag cgctgggcct ggtgatggcg gcgatcctct acgagccggc
     1741 cttcgccgcc gtggcgacct ggttcgagcg cgagcggggc aaggcgctgc tgctgctgac
     1801 cttcgtggcc ggcttcgcct ccacgatctt cctaccgctg gcgagccggc tcgtcggggc
     1861 gctcggctgg cggcaggcgc tcctcgcgct ggcgggcatc ctcgccgtgc tgacgatccc
     1921 cgcccacgcc ctggtgctgc gccgccgtcc cgaggacctc ggcctgctgc ccgacggtca
     1981 ggagcggcgc ggtgcgctcg gcgacgccgc cttctggtgg ctctccggct cgatcatcct
     2041 ggggacggtg acggtggtgg cgctcggggt ccacctggtg ccgctgctgc gcgagcgcgg
     2101 ttacggcgcg gacaccgccg cgctggcgac cggcgcgctg ggggcgctct ccgtgaccgg
     2161 gcgcgtgctc atcaccgcgc tgatccgccg gttgccgcaa gcgctggtga cggcggcgct
     2221 gctggcgctt cagtcgatcg cggtgctggt gctgctggcg tgggacagcc cagccgggct
     2281 ggtgctcttc gtgatcctct tcgggctcgg cttcggcgcg ctaacgttgg cgcgcgccgg
     2341 cctgatcgcc gagtggtacg gccggaagca ctacggggcg attggcggcg cgctcgcgct
     2401 ctgcacgacc ggggcgcggg cgctggcccc ggtcggcgcg ggtgcgctgt atgtgtgggc
     2461 gggcggatat gggcaggtgc tgatctgcgt ggcggtggtc gcggcgcttg cggcgctggc
     2521 gatgatcggc gcgcagttta ccgcgaggcc gctggcgacg agaacgagtg aagaacaccc
     2581 gtgtggcggg tgacgggggg gcgctcggcc cggtgcgtga ccaccccggg caatcattga
     2641 agatattcta ctccgggacg aactgaagag agcttgacag gccgacacgc gcgtggtact
     2701 gtacgtacat cagggacgcg cgagtgtccc gccgccacga ctctcccaaa gggtagcggt
     2761 gtgcggggcg cgggcggacc ggtcgaacag ctctctcggg cgttgggcga cttcagttct
     2821 cacgcgtggt gccgcgaggt gctggtcacg cgagcgatcc tgagcgacgg tatgccgatg
     2881 gcctttcggg gttgtcggca ttcgtcgttc ttggcatttc aaggcatggt tcggtgtgat
     2941 taccacggac gagattcatc agcaggcgcg gcaactcaac atcacccccg ctgcggtcca
     3001 gcgtgactac gtcctgggct ggctcatctt cggagtcttt gctcggagcg ccctgggccg
     3061 ccatctcatc ctcaagggtg gcaatgccct gcgcaaagcg tattttgcga acacgcgatt
     3121 ctccgacgac ctggacttca cgacccagga gaacctcgac ccggagacgc tggcgcggga
     3181 actcgacctc gtgtgccagt tcgtccagga gcacaccggg gtagcattcg accttgagca
     3241 gaatcgggtc gaggagggcc gctttatcac caaagagaag cgggtgtaca aggtccgcct
     3301 ctatttcaga ggcttcgccg agcacttcga acatctgacc ctgcgcgtca atgtggatat
     3361 cactcagaac gaccgactct ccctgccagc gcagatgcgc gccctcatcc acccctattc
     3421 tgatggtgac gattgccgga cggagttgcg gtgcgtgcag ctcgaagagg tgctggccga
     3481 caagctgaag tgccttctcc agcgccggcg atcgcatgac ctctacgacc tcgtccacgc
     3541 gctcttcatc aataccaagg tcgcgatcga ccggaaggcg atcatcgaga cattccttgg
     3601 caagacgatc ttcaagccca gtccgcctgc cctgcgcgac ctcctactgg aagtgccgtt
     3661 cgaggtattt cgcgactatt ggcgcaaggc gatcgcctgc cccgagggca gccacctccc
     3721 attcgactcc gccgtcgacc acttccggca ggagatcccc gcgctcttcg cccccttctc
     3781 ctatggtgag cacgcctcgg tggccttctt cccggccagg ttgcgcaatc cgatcctgga
     3841 agcgggcagc gggtggaagg ttttgcgcgt ggagtacgac ggggtgacgc gcctggtgga
     3901 accctattcg cttgtcttca agcggcgcat ggatgggcac ggccaggagt acctctacgt
     3961 ctacgaccgg actggcggac ggtcgagcgg gccggacatc aagtcgttcg tccactcaaa
     4021 gttgacgcgc gtcgaggtga ccgacgagca gttccagccg cgctttgaag tagaactctc
     4081 caaggccggg gaattcggtg accgctccta cttcggccgg cccttcggcg gcggcacgcg
     4141 agcggcagga caggtccggc gcgctttgag cttctcgggc atgacctatc gaattcggtg
     4201 cccattttgc aataagactt tccgcaggaa gaccatgttc gacgccaggc tgaatgcgca
     4261 caagaacgaa tatggcttcc cttgctccgg gacgattggc tttccggact gatcatctcc
     4321 cagctgccgt ggcgagggct tgccgatcag tttcctgctc tgctagactc gatgcatcaa
     4381 ggttcgtcga tgtattcctg actggatgcc gcgatgatca ccacgctccg ccggacgaac
     4441 gacccgagtg cgccgccgag cttcgcggtg ctggcgcggc agttgaaggc gctcgccgat
     4501 ccaactcgcc tcgcgatcgt cgcgctgcta gcgaagcgtg agcagtgcgt ctgcgacctg
     4561 accgcgacgc tgcgcctgcc gcagagcacc tgctcccacc acctgggggt gctgaagcgc
     4621 gcggggctca tccgcgaccg gcaggatggg cgcgacggac gctggtcgta ctatgcgatc
     4681 gtgcctgagg cagccgaggc gctgcatgga catgtcggca acctgctcga cctgagcggc
     4741 ttcgacccca ctccggcaca ctgcgactga ttcgatcagg ggcgtacacc cgcaggtatt
     4801 catgtggtga gatacatcga cagcgatgga ttcatgggag tagtagtttg agcgcgactc
     4861 cgtcgcagcg cgccgccgcc gaggcggtcg gcaccttcgg acttgtattc gccgggtgcg
     4921 gcgcgatcat gatcgatatg gtgagcggcg gcgcgatcac gcacgtcggc gtggcgctga
     4981 cgttcggcct gattatcacc accatgatct acgcgacagg gcatatctcc ggggcgcact
     5041 tcaatccagc agtgacgctg gcattcgcgg caacgaggca tttcccgctc gccctggtgc
     5101 cggtctacct cgcggcgcaa ttcggcgggg cggtcgcggc gagtctggcg ctgcggctgc
     5161 tcttcggcga cgtggcgcac ctcggcgcga cacttccagc cgggggggca gggcagtcgc
     5221 tggtgctaga agtggtgctg accttcttcc taatgttcgt gatcatcagc gtggcgaccg
     5281 acacgcgggc ggtcggtcag gcggcggcgc tcgcgatcgg cggcacggtc gggctcgaag
     5341 cgatgttcgc cgggccgatc tgcggcgcgt cgatgaaccc ggcgcgctcc ctggcccccg
     5401 cgctggtgtc gggcacctgg acggcgcaat ggctgtacgt cgtcgggccg atcgtcgggg
     5461 cattgctcgg agcgctcatg tatcaaggac tccgggggca ctcgggtgat gcggaaacgc
     5521 tcgtgggggg atagagggat gggacgagag tgggtgctct tcctctgcac gcacaactcg
     5581 gcgcgctcgc agatggcgga ggggctgctg cgacacctgg ccggggatcg ctttgtggcc
     5641 ttgagcgccg gcacggtggc gacggcggtg cgcccgcagg cgatcgaggt aatgcgcgag
     5701 atcgggatcg acatcgcgcg gcatgagtcg aagacgctgg agcgctatct gggcgagccc
     5761 ctcgatctgg tgatcacggt ctgcgacgac gccaacgaag cctgcccgat cttccccggt
     5821 tcgcacgaac gactacactg gtcgtttgag gacccgagcc gggcgaccgg cagtccggag
     5881 gagcagctgg tggtctatcg ccgcgtgcgc gacgagatcc gcgcgcggat ggaaagggag
     5941 ctattggggc gaccgccttc ggagagatcg gagcgacgcg ttctggggca gtcgtaggcg
     6001 ctcgcggccg cacaggataa tcaatccgca ggcccgataa cagccattac cagggtaaat
     6061 gaggtggctg gcagcaactc tcccacaatg cgaccccata cctattgaac ttggtcaata
     6121 agtgccggcc aatttgctca ggtgccgtcc ccgagcggcg tcactctccc cctttcgccg
     6181 tccaccagca cgcgctgccc ctcgcgcagc cagcgggtcg cgttgctcgc tcccatcacg
     6241 gccgggatac cgtactcgcg ggcgacgatg gcggcatggg aggctgcgcc gccggcctcc
     6301 acaacgacgg cgctggccac ggcgaagagc ggcgtccagg ccgggttggt gtagggggcg
     6361 accagcacct cgcccggctg caaccgccag aaatcgcctt cgcgcaggat aatcctgacc
     6421 gacccgaccg ctcgccccgg gctggctgac gcgccgacca acgcgccgtc ttggacggtc
     6481 tgcgccacca agtcggccgg cacggcggtg tagtggtcct cgaccgctcg ccgggccgct
     6541 tggcgctgaa ggactcgttc ccggaggtcg tcggtcgcat tcagcgggcg cagctccgcc
     6601 agttccagga agaagatatc tgagtccgcc gcgagcaccc cgcgcgcaac caaacgctgc
     6661 cccaactcca gcgccagccg acgcaggacg gtgaacggga ggaagaggta gaagtggctg
     6721 tcctcgcgga aggcgacgga gcgccgcgcc gccgcgaccg cgcgcactac gagcgggcgc
     6781 aggaaccgcc gacggtcgag ggcggcgtcc agttcacgcc tggcccccgc gacccgctcg
     6841 gggtcgtcgt ccaacgccgt cgccgcctgc tcgccgacca cgagcccgcg gaggaggccg
     6901 tagacgacgg ccggctcgtc gcgccaggcc gggaaacctg cccccgccat cgcggtctct
     6961 cgccagccgt agcgccggag gaaggcctcg atatccgcgc gcaccgccgc cccgccggcg
     7021 gtcgcatgca ggcggtcggg caactcgtcc ggaggcgtgg tcaggaaagc cgcgcgcacg
     7081 tcggccgccg cgcgcacctg ccgggcgagc tgccccagcg cccggttcgc ctcagtggtg
     7141 atacatggca cggcagcgag gaggtcggcg tgcaggcggt cagcggcgcg cttcccgacc
     7201 gccaggcgca gcagcgacgg caaggccagg ccgactaata gcccgggcac gaggaacccg
     7261 aagcggtgca tgaagtgccg cagctgcagg ccttgcagtt cttcgatgcg ggccagaatc
     7321 gctgcgtcgg cgagcggggc caggtcctcc gcgtccagtt cctcggcgcg ggctaccagg
     7381 gtcgcgcgga cggccgcgag ccaggggtca gtcgcggcgc gcagcgcggc caccagcttc
     7441 gccgggagtg tgagcgcgcg gagagtcggc cggacggtcg gcgggaccat ctgaacgacg
     7501 ccgggagcga cttcaataag cacgtccccc accggtggcg gcacgagccc gaggccgcgc
     7561 aaggactcca gcacgcgcgt caggagcggc cggaaataga gggtgacgtc gaggggatag
     7621 ggcggccggg gcaggtggtc gagcgcgttc ggcgcgccag cgcgctgaaa gcgactgtag
     7681 cgtcgcgcgg cgaccggagt tgtctcggac ggtagggcgg tgagcgggcg ggcctggagc
     7741 acgacaaccc gcccgttcgc gtaggcccac tcgatatcct ggggccgtgc aaaggctgcc
     7801 tcgatgcgct gacccaaccg ggcgaggtcg gccgctaggt gccggggcag ggccgcgtcg
     7861 ccggatagaa cccgctggcc acgctttgtg acgacgtagc ggtgcccggt ggcctcgcct
     7921 gagaccagcg cctcgcccaa gcctggtacc gcctcgagaa cgacccggtc gcgtcgcccg
     7981 gacaccgggt cggcggtgaa gaggacgccg gcccactccg ccgggaccat cacttggacg
     8041 accacggcga gggcgaggtg ggggatggcc cacccgtgcc gccggcggta gtccacggcg
     8101 cgcggcgacc agagcgaggc ccagcacgcc cggacggcgt cgaggagccc ggcctctccc
     8161 atgacattga ggaaggtgtc gtgctgcccg gcgaaggagg cggccgccag gtcctcggcg
     8221 gtgccggagg agcggaccgc gacggccgtg gccccgagcc gccggtaggc gcccagcagc
     8281 ggcgtgctga cctcggtcgg cagcgggagc gcgggcaggc gggtgcgcag ctcctccggg
     8341 tcggtagcag cgagttggcc ggcggttact gcgacgcggt acgccgccgt ggtgacgacg
     8401 aagcccggcg gcaccgggaa gcccaagcgc accagccggc ccaggttcgc ccccttgccg
     8461 ccgaccaagt c
//
LOCUS       DASUBI010000845         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_198347, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000845 DASUBI010000000
VERSION     DASUBI010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198347"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VFU72_16755"
     CDS             <1..1679
                     /locus_tag="VFU72_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5425193.1"
                     /translation="QRGRTGFASQSGALGLVAVEQARAFGLGISSFISVGNKADISGN
                     DLLNYWESDPDTDVVMLYLESFGNPIKFSRIARRVGHTKPIIAVKSGRSKAGARATSS
                     HTGALLAASDVTVDALFRQAGVIRANTFEEMFATAALLGNQPLPRGHRVAIVTNVGGP
                     AILCADACEGLGLELPVLSEATQERLRAFLPEEASVGNPVDLIASATAEQYRQAIEAV
                     TADPGVDAVVAMFIPPLATRTEEIAAAITEATQAGGDDKPLVAVFMTADRPATIPAAS
                     PRQVPVYSFPETAATALANATRYAIWRARPLTPPGRPPGLRTDEAAAIVAGALGRGGG
                     WLEPAEVAALLACYGIPMVESVSVGTPDEAAAAARTLGDEVALKAIAPGLSHKTELGA
                     VRLGLRGPAQVGQAAREMAERLTGAGYTPSGFLLQPMIRGGVEMLVGIVRDPSFGPVV
                     AIGAGGTLTELLQDVSVRLAPFVGDEPRAMIRELKTYPLLDGYRGGPHYDIAALEDIL
                     LRAGALAEDLPPVIECDLNPVVVLEASAVVLDARVRVAPLRPPEPAPHPA"
     gene            complement(1683..2102)
                     /locus_tag="VFU72_16760"
     CDS             complement(1683..2102)
                     /locus_tag="VFU72_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5425194.1"
                     /translation="MAIVEADRALGELLRDAFREEGYDAALCPHRSAATATIHVTNPD
                     LVLINSWLGRRDDGWKLVAELRGQVATERLPVIVFSSDLENPATLRDRCRQSRLCAIV
                     APFDLENLLARAAECLAAGERAAGARQLAAAPGGARS"
     gene            2515..3399
                     /locus_tag="VFU72_16765"
     CDS             2515..3399
                     /locus_tag="VFU72_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5425195.1"
                     /translation="MEPVVKTILLATDGSPDSLLATRAAADLAVLTSATLHVIHVWSQ
                     LTPVTVYPYPAVTEDFIKLYEDQEQAVLAEAVERIAAMGGPPAVPHLQMGRPVDEILA
                     LGRELGADLIVAGSHGRGAFGRVVIGSVAEGLTHDADVPVLVVRGGEQSWPPQRVVVG
                     DDGSDEALAAAAFAFRLGRLFDASCLLVHVIPDAPISWRRDVGDFQELLRRTEKVAAE
                     RAHELGQRSETQPEPVVRVGDPATELAAIADEGAATLVAVGKRGLGRIERLRLGSVST
                     KVLRAVAVPVLIGPNQAG"
     gene            3452..3868
                     /locus_tag="VFU72_16770"
     CDS             3452..3868
                     /locus_tag="VFU72_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5425196.1"
                     /translation="MVKAQPHQTSYGFGEQVSLPYAEAVEQVKAALKQEGFGVLCEID
                     VRRTLKEKLGVDTRPYVILGACNPPLAHQALSVEPDLGLLLPCNVVVYETEGGSIVEA
                     MDPVPVLGLVGNDGLEEIAQEVRRRLQRAIGSLPAG"
     gene            complement(3894..>4447)
                     /locus_tag="VFU72_16775"
     CDS             complement(3894..>4447)
                     /locus_tag="VFU72_16775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5425197.1"
                     /translation="ALMGLAFIFVLLGMPYSQLLPVFQQNVFHVGPERLGFMYTAVGL
                     GAVVGSLTAAYLAESTKKRTIQIVVGCIFGLMLAAFALSPSYVIGLPFLLIAGGMIET
                     YFTLNRILVILNTDRAVFGRVMAIYSMSWSLTPVSLLVMGAIVDQVGAPLTVATAGVA
                     LAILVVALALSIPGVRHVGEMGH"
BASE COUNT          676 a         1565 c         1596 g          610 t
ORIGIN      
        1 cccagcgcgg acgcaccggc ttcgcctcgc agagcggggc gctggggctg gtggccgtcg
       61 agcaggcgcg cgcgttcggc ctgggcatct catcgttcat ctccgtgggc aacaaggccg
      121 atatctccgg caacgacctg ctcaactact gggagagcga ccccgacacc gacgtggtca
      181 tgctctatct ggagtcgttc gggaatccga tcaagttctc gcgcatcgcg cggcgcgtcg
      241 gccacaccaa gccgatcatc gccgtcaaga gcggccgctc gaaggccggc gcgcgcgcca
      301 cctcctcgca caccggcgcg ctcttggctg cctccgacgt caccgtcgac gcgctcttcc
      361 gccaggcggg cgtgatccgc gccaacacct tcgaggagat gttcgcgacc gcggcgctgc
      421 tggggaacca gccgctgccg cgcggccacc gggtcgcgat cgtgaccaat gtcggcggcc
      481 cggccatcct ctgcgccgac gcctgcgagg ggctcggtct ggagctgccg gtcctgagcg
      541 aggcgaccca ggagcggctg cgcgcctttc tgcccgagga ggccagcgtc ggcaatcccg
      601 tcgacctgat cgcctcggcc accgcggagc agtaccggca ggccatcgaa gccgtcacgg
      661 cggaccccgg tgtcgatgcc gtggtggcga tgttcatccc cccgctggcc acgcgcaccg
      721 aggagatcgc ggcggcgatc accgaggcca cccaggccgg cggcgacgac aagccgctgg
      781 tggccgtctt catgaccgcc gatcgcccgg ccaccatccc ggccgcgtcg ccgcgccagg
      841 tgccggtcta ctcgttcccg gagacggccg ccaccgcgct ggccaacgcg acgcgctacg
      901 ccatctggcg ggcgcggccg ctcacgccac ccggccgacc gccgggcctg cggaccgatg
      961 aggccgcggc catcgtggcc ggcgcgctgg ggcgcggcgg cggctggcta gaaccggccg
     1021 aggtcgcggc gctgctcgcc tgctacggca tccccatggt cgagagcgtc tccgtgggta
     1081 cgccggacga ggccgcggcg gcggcgcgca cgctgggcga cgaggtcgcg ctgaaggcga
     1141 tcgcgccggg gctgagccac aagacggagc tgggcgccgt ccggctgggg ctgcgcgggc
     1201 cggcgcaggt cgggcaagcc gcgcgtgaga tggcggagcg cctgacgggg gccggctaca
     1261 cgcccagcgg cttcctgctg cagcccatga tccgcggcgg cgtggagatg ctagtgggga
     1321 tcgtgcgcga tccgagcttc ggcccggtgg tcgccatcgg cgcgggcggc accctcaccg
     1381 agctgctgca agacgtgagc gtgcgcctgg cccccttcgt gggcgacgag ccgcgggcga
     1441 tgatccgcga gctgaagacc tatccgctgc tggacggcta ccgcggcggc ccgcactacg
     1501 acatcgccgc gctggaggac atcctgctgc gggccggcgc gctggccgag gatctgcccc
     1561 cggtgatcga gtgcgatctg aacccggtgg tggtgctgga ggcgagcgcg gtggtgctcg
     1621 acgcgcgcgt gcgggtcgcc ccgctgcggc cgcccgagcc cgcgccgcac ccggcgtagt
     1681 agtcaggagc gagcgccgcc aggcgcggcg gccaactgcc gcgccccggc ggctcgctcg
     1741 ccggcagcca gacactcggc ggcccgcgcc aggagattct ccaggtcgaa cggcgcgacg
     1801 atcgcgcaca gtcgtgactg gcggcagcgg tcgcgcagcg tcgccgggtt ctccaggtcg
     1861 gacgagaaca cgatgaccgg gagccgttcc gtcgcgacct ggccacggag ctccgccacg
     1921 agtttccagc cgtcgtcgcg cctgccaagc caactattga tgagcaccag atcggggttg
     1981 gtgacatgga tggtcgcggt cgcggcggag cgatgggggc agagcgccgc gtcgtagcct
     2041 tcctccctga aggcatcgcg taacagctca cccagcgctc gatcggcctc gacgattgcc
     2101 accagtgggg cccgatcgct ccacgttcgc cccatcatgc ttgccccttc cgatgctgta
     2161 ccggacgtcc cgacctcgaa cggcggctgt gcgcgggttg tagatacaga ttctggcttg
     2221 atcggtatca gacacagtac aaccggacgt gacgacgtga caaattcgtg tcaatccgtt
     2281 ccgcgctcgc cgcgctggca cgcaagctgc ccgaaacacc tgaggcgccg cgccgccgtt
     2341 tcgccgaagg gcggtcccag gcccgcgtct acggcgagca ccgacgaatg tcacacgcct
     2401 gtaatcccgc gctcgtgcct gatacggccc cgtcgcgcgg cccggcttat gctcgacgcg
     2461 tatggcggcg gcgcgcgccg acgagcgccc ctacgatcag ggaggacacg cacgatggag
     2521 cctgtagtca agacgatcct gctggcgacg gacggctcgc ccgactcgct gctggcgacg
     2581 cgcgcggcgg ccgacctcgc cgtgctcacc agcgccacgc tgcacgtcat ccacgtctgg
     2641 tcgcagctca cgccggtgac cgtctacccc tacccggcgg tgaccgagga tttcatcaag
     2701 ctttacgagg atcaagagca ggcggtgttg gccgaggcgg tggagcgcat cgccgccatg
     2761 ggcgggccgc cggccgtgcc ccatctccag atggggcgcc cggtcgacga gatcctggcg
     2821 ctcgggcggg agctcggcgc tgatctgatc gtcgccggca gccacgggcg cggggcgttc
     2881 ggccgggtcg tgatcgggag tgtggccgaa gggctcacgc acgacgccga cgtcccggtc
     2941 ctggtcgtgc gcggcggcga gcaatcctgg ccgccacagc gcgtcgtggt cggcgatgat
     3001 ggctcggacg aggccctggc ggccgccgcc ttcgccttcc gcctcgggcg gctcttcgac
     3061 gcctcctgcc tgctcgtgca cgtgataccc gacgcgccga tcagctggcg gcgcgatgtc
     3121 ggcgacttcc aggaactcct gcgacgcacg gagaaagttg ccgccgagcg ggcgcacgag
     3181 ctcgggcagc ggagcgagac gcagcccgag ccggtcgtgc gcgtcggcga tcccgcgacc
     3241 gagctggccg cgatcgccga cgagggcgcg gcgacgctgg tggccgtggg caagcgcggg
     3301 ctggggcgaa tcgagcgcct gcgcctgggc agcgtctcga ccaaggtgct gcgcgccgtc
     3361 gccgtccccg tcctgatcgg cccgaatcag gccggctgat cgaaccggag cgggtgcttc
     3421 ggccggtcga gcgcggagag aggatcacgt gatggtgaag gcacaacccc accagaccag
     3481 ctatggcttt ggcgagcagg tgtcgctgcc ctacgccgag gcggtcgagc aggtcaaggc
     3541 ggcgctgaag caggaagggt tcggcgtgct gtgcgagatc gacgtgcgcc gcacgctcaa
     3601 agagaagctg ggcgtcgaca cccgtcccta cgtcatcctc ggggcctgca acccgccgct
     3661 ggcccaccag gcgctcagcg tcgagcccga tctcggcctg ctgctgccgt gcaacgtggt
     3721 cgtctacgag accgagggcg gcagcatcgt cgaggccatg gatccggtgc cggtgctggg
     3781 cctggtcggc aacgacgggc tcgaggagat cgcccaggag gtccggcgcc ggctgcaacg
     3841 cgccatcggc agcctgcccg cagggtaggc gaacccgaac ggcgaacgcg ccgctagtgc
     3901 cccatctcgc ccacgtggcg cacgccgggg atggacaggg ccagggcgac gacgaggatg
     3961 gccagggcga cgccggcggt ggccaccgtc agcggggcgc cgacctgatc gacgatcgcg
     4021 cccatcacca gcagcgagac cggcgtcagc gaccagctca tcgagtagat cgccatgacg
     4081 cggccgaaga cggcgcgatc cgtattcagg atcaccagga tgcggttcag cgtgaagtag
     4141 gtctcgatca tgccgccggc gatcaagagg aagggcaagc cgatcacgta gctcggcgag
     4201 agcgcgaagg ccgccagcat caggccgaag atgcagccga ccacgatctg aatggtgcgc
     4261 ttcttggtcg actcggccag ataggcggct gtcagcgagc cgaccaccgc tcccagcccc
     4321 acggcggtgt acatgaagcc cagccgttcc ggcccgacgt ggaagacgtt ctgctggaag
     4381 accggcagga gctggctata gggcatgccc agcagcacga agatgaaggc caagcccatc
     4441 aaggcca
//
LOCUS       DASUBI010000846        10342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_226537, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000846 DASUBI010000000
VERSION     DASUBI010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226537"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..709
                     /gene="pdxT"
                     /locus_tag="VFU72_16780"
     CDS             89..709
                     /gene="pdxT"
                     /locus_tag="VFU72_16780"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5425198.1"
                     /translation="MTVIGVLALQGAFIEHVKKLHALGVVVREVRLPADLEEIDGLII
                     PGGESTTIGKLIDRFELRQPILDRAHAGMPIWGTCAGMILLGQEVDADTQARSQPLLH
                     LMDIGVRRNAFGSQLDSFETELDFPVLGPEPLPAVFIRAPIISWIGPEVEVLAKLPDG
                     RIVAARQDQYLATSFHPELTADNRLHAWFVALAAQASRRPAAQATS"
     gene            895..1110
                     /locus_tag="VFU72_16785"
     CDS             895..1110
                     /locus_tag="VFU72_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5425199.1"
                     /translation="MRGDERRGYTVEEAAEILQYHPDALRYWLRVGQLSGVRGAAGEE
                     WLIDLEALLVFLRQSDDPVPIVLPLAS"
     gene            1225..2916
                     /locus_tag="VFU72_16790"
     CDS             1225..2916
                     /locus_tag="VFU72_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEU5425200.1"
                     /translation="MPIPRPLFLTGQNDAYTDAAETVPAKLTAHARTMAQGVYQIAEQ
                     FYVAVGYGQSNMTMVVGTDGVILIDCLETEEHARQALADLRQVSGSGQPVKALVYSHS
                     HPDHISGVRALLDPAAIERGQVQIYAHERLPHVIRGNPSLGLVPPLRLAYTFGLGTTG
                     FLPPTTLFQGSLNATVAGLTFQLHEAPSESDDEIVVWFPDQRVLFVADVIQGESLPNL
                     YALRGAVRNPLEWVRALDLLRTYDAEALIFGHGRPLIGLGEIQDLLVKYRDALQYIHD
                     QAVRLMARGLTPDELIEALGELPPRLREHPWLGEFYGTVKQTVPQIYANYYGWFEGDP
                     TFIDRLPRREQAARYVAAMGGRDPVVAVAEHAYAEGDYQWVAEILTHLLRVDSEDQDA
                     RRLKAQALRRLGYLTSNPIWRNNYLMGAREIDGTLDRDRLLKTVRAMGNPDIAAAMPI
                     PLLLRAFATRLNPSRSDGSFLQVAFRCTDSGADYGLAIRSEVAEVLPVAAPHSTIVVE
                     TTEPTLRGLLTGRLPWPRAVEEGAATLEAGTPEEATRFWSLFDSPLGQLPNLALR"
     gene            complement(3135..4271)
                     /gene="rodA"
                     /locus_tag="VFU72_16795"
     CDS             complement(3135..4271)
                     /gene="rodA"
                     /locus_tag="VFU72_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU5425201.1"
                     /translation="MGTLITSLGVRRQATALTLDLYLIVTTAVLIGFGLIAIWSADGG
                     GPLAGNLVVRQALYVLIGIPVVALITSIDYRYLQSLAWLIYAGTLAMLTLVTLMGTSV
                     AGGTRWIMVGPITFQPSEIAKLGVIIALAAFIADRGEEMRRFVNFVLAGVLVAVPMAL
                     VERQPDLGTASVFAFIWLVMILMSRARLRYLLGLILCSPVVALVGWRFLLHDFQRERL
                     LISYHPERDYLGAGYNIIQAQITIGSGGFLGHGLAGSSQSQLDLLRVRTTDFIFAHAM
                     GMFGFIGAVALYLTYAILIWRALQVVGVARDNFGQLVAVGIAAMIFFQVFINTGMNMG
                     IMPVTGIPLPFITFGGSSLWTLLAGIGLLQSIRIHHQRLGFQPE"
     gene            4551..7016
                     /locus_tag="VFU72_16800"
     CDS             4551..7016
                     /locus_tag="VFU72_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425202.1"
                     /translation="MPRLLTASPTLVLQRGQPALASPYLALEGCARISAPGSETIALV
                     LEPVEESPRARQVAQDLAERLIEEIQARRGQTTTAALLAAIELINGWLVQLNAVRPPR
                     ERCYFGFPCVLTRDDEAYVAQAAPSQILIAQEGEIYAFPGLESWRWHTRETTDSTAVA
                     LPLGLGETQPDLYHTRLAAGDLLVLCSSSLARVIDRAPQVAFAQGDTAIVLDHLDELA
                     RLFAVSDAWAAAVVVPEPVRQERRHERGGDLAFLRRASSWFSHFLPEETAERLRRHTG
                     NSDPDEVDLSDEAVHTVDYTAGMTSSDVAESLYWDDARGSTADFPIVTGATAPSADAL
                     LDEAPTRPDDEPDERYWEPETGDPHDIPDDWESDAALEDAAERPARGRGVNDLLAVAL
                     LAFSAAVVGVRQLTINRDRSLGPRDDGTLGVPRLSRYDDNIQLPEFRGVRSRMPRVPL
                     SRTVALALVILLITFGAAVTVSVRNKHERQRQARMTALFQNAESLRQRATESSDPAAV
                     QNILQAASASITRAEAFGLDQDQAAREQTAISADRDQALKIDRLANIQVLGSLPAAPR
                     GVTPRIFAGNGQIYVFTDALYRLDANGNALVRLIGGGDQIGGQPVATLLGAAWGDGAP
                     VVFDGSRAYLFDPPTGSWHAQQVETLGGPETGIAAINGYSGNLYLLSPQTGQIVKYGA
                     GAYDNQPEDWTAGAATDDLKQAVDFVIDGHIYVLTKDGRVIDFFMSAEQSTVTPHAVP
                     PVTNAVALSLQTDRPYVYIAERDRILRLERSSGNLVQQFVAGSDAPPFNDIRAFSVDD
                     VLGTAYILNGNALLSVRLPGPPH"
     gene            7269..7679
                     /locus_tag="VFU72_16805"
     CDS             7269..7679
                     /locus_tag="VFU72_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425203.1"
                     /translation="MSLEQLRGSAMMEHLVDALGQGKDIGHYGRLTVAMVGHFYLDKH
                     ELLQLLEQGQGASEEDLMALVQEVEARDYNPPTRGQILDWQQQQDFPICPHPEDPDAC
                     NVYQELKLPERVYAQIKEYRAEQFEQEEQAEQHG"
     gene            complement(7697..8401)
                     /locus_tag="VFU72_16810"
     CDS             complement(7697..8401)
                     /locus_tag="VFU72_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425204.1"
                     /translation="MAEASPPVAPIPELIALRIAGSSLTRFAHTTLPLQRGDWVAFPG
                     LYGEEPARVAAAPGEVEAGPLAEPWPEVTRRLEDDEVTRVERQAERALEQTAQVARLA
                     ANEFSGLIPTGLRYALDGRAICSLQAPDALDLAAFGARLATLLGVPVELEREPAGAPL
                     VGSLGRLRPEAPELATLVRDRLQRLGAAPTLGSRVRFPGGVGQFIAASIPRNEARVRL
                     PDGDEVVVSLDDIAPA"
     gene            complement(8377..9423)
                     /locus_tag="VFU72_16815"
     CDS             complement(8377..9423)
                     /locus_tag="VFU72_16815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU5425205.1"
                     /translation="MTLTGAEAGSWPVVGHADAVALLSRAVGGGQVGHAYLISGPEGI
                     GRHTLAVAFTQALLCTAERKPCGVCSACSRVARGLHPDVLTLSLETQAAESKRDAKNT
                     QISIESVRELRTALALRPLEGPWRVALLEDVERFSWPAFDALLKTLEEPPPFVVLLLI
                     ATEVERVPQTIRSRCRPVPLEPLPRAEVAAQLRARGVEPDQAASLAALTRGRLGQALA
                     LADDPKARDEREKAINAALAMIENPLEALKAARSLADNFRRGKRDEVAAQLDTLLGLW
                     RDILLAAGGCAEQMVNADVAARIEALALRWSLPEIQQALHATYDALTDLSINVQPRLA
                     LDRMVTQWPKPARR"
     gene            complement(9433..10212)
                     /locus_tag="VFU72_16820"
     CDS             complement(9433..10212)
                     /locus_tag="VFU72_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425206.1"
                     /translation="MRVVRNTGYIKTRQRAGRLVAWGGGLALLFSLVLTFVTPGLILP
                     AYVVLILGFIGFQYGMQQVTKWSRKPSADQVLDNALRRLNDRFTLIHYSDAVPGHPEH
                     ILVGPAGLIVITTRDVFGQVKVKDNRFHRAGGRLWFLVGMGSPQLGNPTAESEREQKN
                     LADFLTTHNLAGADDIEGVITFLPNPRRPLELEVISSDITVVTLDQLYNAIRDLSGDT
                     MLTKQQRDEIVEALSQGEGIEGPQTLPTGPQPGAKKRARAA"
BASE COUNT         1661 a         3556 c         3557 g         1568 t
ORIGIN      
        1 cttctcccag cattgggaga aggggcgggg gggatgaggg cactcggggg cggaggatga
       61 gggccgctcg cccgaaaggg atcacgtcat gaccgttatc ggagtgctcg ccctgcaggg
      121 ggccttcatt gagcacgtca aaaagttgca cgcgctgggc gtcgtggtgc gcgaggtgcg
      181 gctgccggcc gacctggagg agatcgacgg gctgatcatc cccggtggtg agagcaccac
      241 catcggcaag ctgatcgacc gcttcgaact gcgccagccg atcctcgacc gtgcccacgc
      301 cggcatgccg atctggggta cctgcgccgg catgatcctc ctgggccagg aggtcgacgc
      361 cgacacccag gcgcgctcgc agccgttgct ccacttgatg gacatcggcg tgcggcgcaa
      421 cgccttcggc tcgcagctcg acagtttcga gaccgagctg gacttcccgg tgctcgggcc
      481 ggagccgctg ccggccgtct tcatccgcgc cccgatcatc tcctggatcg ggccggaggt
      541 cgaggtgctg gcgaagctgc ccgacggccg catcgtcgcc gcgcgtcagg atcagtatct
      601 ggcgacctcg ttccaccccg agctgaccgc cgacaaccgg ctgcacgcct ggttcgtcgc
      661 gctggcggcc caggctagcc ggcgtcccgc ggcccaggcg accagctaga ccacagctca
      721 cgccaccgca agggcgggtt cgaaacccgc ccctgcaatc tcccgttctc ccatgattga
      781 aagggggcat gagggatgag gacgccccgt cagccccccg tcccctgttc aacccccatg
      841 ccagctgcta cactgtgtgt acgtacacgt ctcgcgcgtg atggaggagc ggtgatgcgg
      901 ggagatgagc ggcggggtta cacggtcgag gaggcggcgg agatcctgca ataccacccg
      961 gacgcgctgc gctactggct gcgcgtcggc cagctcagcg gggtacgcgg cgctgctggc
     1021 gaggagtggc tgatcgacct ggaggcgttg ctggtcttcc tgcgccagag cgacgacccg
     1081 gtcccgatcg tcttgcccct ggcgagctag cgcgctcggc cacggccgcg gagcccacgc
     1141 ctcgtcagcg cttgtagcgg ttggcgaggc gtgctagcgt cggtcagaca atgagcgagc
     1201 acgacgttcg gtctggaggg tgtcatgccg attccaaggc cgctcttctt gaccggtcaa
     1261 aacgacgcct acaccgacgc cgccgagacg gttcccgcca aactcacggc tcacgcgcgc
     1321 acgatggcgc agggcgtcta tcagatcgcc gagcagttct acgtcgccgt cggttacgga
     1381 cagtccaaca tgaccatggt cgtcggaacc gacggggtca tcctcatcga ttgcttggag
     1441 accgaagagc acgcacggca ggcgctggcc gacctgcgcc aggtcagcgg cagcggccag
     1501 ccggtcaagg cgctcgtcta tagccacagc caccccgacc acatctcagg ggtgcgcgcg
     1561 ctgctcgacc cggccgcgat cgagcgcggc caggtgcaga tctatgccca cgagcgcctg
     1621 ccgcacgtaa tccgagggaa tccgagcctc gggctcgtgc cgccgttacg tctggcctac
     1681 accttcggcc tcggcacgac cggcttcctg ccgccaacca cgctctttca ggggtcgctc
     1741 aacgcgacgg tagccggctt gaccttccag ctgcatgagg cgccgagtga gtcggacgac
     1801 gagatcgtcg tctggttccc cgaccagcgc gttctcttcg tggcggacgt gatccagggc
     1861 gaatcgctgc cgaatctcta cgccctgcgc ggcgccgtcc gtaacccctt ggagtgggtg
     1921 cgcgcgctcg acctgctgcg cacctatgac gcagaggcgc tgatcttcgg ccacggccgc
     1981 ccgctcatag ggctgggcga gattcaggac ctgctcgtga agtatcggga cgcgctgcag
     2041 tacatccacg accaggccgt gcggctgatg gcgcgcggat tgacgcccga cgagctgatc
     2101 gaggccctcg gtgagctgcc gcctcgcctg cgtgagcatc cctggcttgg tgagttctac
     2161 ggcacggtca agcagacggt gccgcagatc tacgccaact actacggctg gttcgagggg
     2221 gaccccacct tcatcgatcg cctgccgcgt cgggagcaag cggcgcgcta tgtcgcggcg
     2281 atgggcgggc gcgatccggt cgtcgccgtg gctgagcacg cctacgcgga aggtgactac
     2341 cagtgggtcg cggagatcct gactcacctg ctccgcgtgg actccgagga tcaggacgcg
     2401 cgccggctca aggcgcaagc gctgcgccgg ctcggctacc tgacgagcaa ccccatctgg
     2461 cgcaacaact acctgatggg cgcaagggag atcgacggca cgctcgatcg tgacaggctg
     2521 ctcaagacgg tgcgcgcgat gggcaaccca gacatcgcgg cggccatgcc gattcccctg
     2581 ctcctgcgcg ccttcgcaac ccgactgaac ccgtcgcgaa gcgatggctc gttcctgcag
     2641 gtggcctttc gctgcaccga tagcggcgcc gactacgggc tcgcgatccg ctccgaggtg
     2701 gcagaggttc tgcccgtcgc tgctccgcac tcgaccatcg tggtcgagac cacggaacca
     2761 acgctgcgcg gcctgttgac cggccggctc ccctggcccc gtgccgtgga ggagggcgcc
     2821 gcgacgctcg aagcgggcac gcctgaggag gcgacgcgct tttggagcct gttcgactcg
     2881 ccgctcggac aactgccaaa tcttgcgctg cgctagcgcg atttccattg ttttcgaccg
     2941 atgcgacgtt cttcctgcag gggcagaccc ctgtgtctgc cctgttgccc cgatcgcgag
     3001 gcaacggagg gaaggggcca cgttggcggt gttggtgaag gttgctgccg ggccatgagg
     3061 gcagacacag gggtctgccc ctacagcgcc taaccgttac actggcggcg ttgatctgcg
     3121 attgacgaag ttgctcactc tggttggaag ccgagccttt gatggtggat gcggatgctc
     3181 tggaggaggc cgatgccggc cagcagcgtc cagagcgagc tgccgccgaa ggtgatgaag
     3241 gggagcggga tgccagtcac cggcatgatc cccatgttca tcccggtatt gatgaagacc
     3301 tggaagaaga tcatggcggc gatgccgact gcgacgagct ggccgaaatt gtcgcgcgcc
     3361 acgccgacaa cctgcagcgc gcgccagatc aggatggcgt aggtcaggta gagcgccacc
     3421 gccccgataa agccgaacat ccccatggcg tgcgcgaaga tgaagtcggt ggtgcgcacg
     3481 cgcagcaggt cgagctggct ctggctgctg ccggccaggc catggccgag gaacccgccc
     3541 gagccgatgg tgatctgcgc ctggatgatg ttgtagccgg cgccgaggta gtcgcgctcc
     3601 ggatggtagg agatcaagag gcgctcgcgc tggaagtcgt gcagcaggaa acgccagccg
     3661 accagcgcca cgaccggtga gcagaggatg agccccaaca ggtagcgcag ccgggcccgg
     3721 ctcatcaaga tcatcaccag ccagataaag gcgaagacgc tggcggtgcc caggtcgggc
     3781 tggcgctcga ccaatgccat cggcaccgcc accaggacgc cggccagcac gaagttgacg
     3841 aagcggcgca tctcctcgcc ccggtcggcg atgaaggccg ccagcgcgat gatcacgccc
     3901 agcttggcga tctccgaggg ctggaaggtg atcgggccga ccatgatcca gcgtgtgccg
     3961 ccggccacgc tggtccccat cagcgtgacc agggtcagca tcgccagcgt gccggcgtag
     4021 atcagccagg ccaggctctg caggtagcgg tagtcgatcg aggtgatcag ggccacgacc
     4081 gggatgccga tcagcacgta gagcgcctgg cgcacgacca gattgccggc cagcgggccg
     4141 ccgccgtcgg cgctccagat ggcgatcagg ccgaagccga tcaggaccgc cgtggtcacg
     4201 atcaggtaga ggtcgagcgt cagcgcggtc gcctggcgcc gcacgccgag actggtgatc
     4261 agggttccca cacggatcct tcccgttccc tcgcggccta ttatagccgt ccggcagcgg
     4321 gctgaccgcg agcccgtctc cgaagcggac cggcgatctg tccgttcact ttgaacgctc
     4381 tagacgtacg tacagtccta tgctagactc gtgggtgccg gacagtcaac gccgcaccac
     4441 tccaacgccg gccgctgctg caatcttgca tattcgtcgt gtcacggcca gtcgccttcg
     4501 cggcggcact ccgggctgtc gatgcactcc ttgaaggagc agggtacgcg atgccgcgac
     4561 ttctcaccgc cagcccgacc ctggtcttgc agcgcggcca acccgcgctg gcttccccgt
     4621 atctcgccct ggagggctgc gcacgcatca gcgcgccggg gagtgagacg atcgcgctgg
     4681 tgctggagcc ggtcgaggag tcgcctcgcg cgcggcaggt ggcgcaggat ctggccgagc
     4741 ggctgatcga ggagattcag gccaggcgcg gacagacgac aacggccgcc ctgctggccg
     4801 cgatcgagct gatcaacggc tggctggtcc agctcaacgc cgtgcggccg ccgcgcgagc
     4861 gctgctactt tggcttcccc tgcgtgctga cccgcgatga cgaggcctac gtggcgcagg
     4921 ccgctcccag ccagatcctg atcgcgcagg agggcgagat ctacgccttt ccaggactgg
     4981 agagctggcg ctggcacacc cgcgagacga ccgatagcac cgccgtcgcg ctgccgctgg
     5041 gcctgggcga gacgcagccc gacctctacc acacccgcct ggcagccggc gatctcctgg
     5101 tcctctgctc gtcctcactg gcgcgcgtga tcgaccgcgc cccgcaggtc gccttcgcgc
     5161 agggcgacac ggcgatcgtg ctcgaccacc tcgacgagct ggcccgcctg ttcgcggtca
     5221 gcgacgcctg ggcggccgcg gtggtggtgc ccgagccggt acggcaggag cgccggcacg
     5281 agcgcggtgg cgatctcgcc ttcctgcgcc gggccagttc ctggttctcg cacttcctgc
     5341 ccgaggagac cgccgaacga ctgcgtcgtc acacgggtaa ttccgacccc gacgaggttg
     5401 acctgagcga cgaagcggtg cacactgttg actacacggc agggatgacc tcaagcgatg
     5461 tcgcggagag cctctactgg gatgatgcgc gtggaagcac ggccgacttt cccatcgtca
     5521 caggagccac tgcgccctct gccgacgcgc tcctcgacga agcgccgacg cgacccgacg
     5581 acgagcccga tgagcgctac tgggagccgg agacagggga tccgcacgac atcccagacg
     5641 actgggagtc ggatgccgcg ctggaggatg cagccgagcg gcctgcgcgg gggcgtggcg
     5701 tgaacgatct gctggcggtg gccctgctgg ccttctcggc tgccgtcgtc ggcgtgcgcc
     5761 agctcaccat caaccgcgat cgctcgctgg gcccgcgcga cgacggcacg ctcggtgtgc
     5821 cgcgcctctc gcgctacgac gacaacatcc agctgccgga gttccgcggc gtgcgcagcc
     5881 ggatgccgcg tgtgccgctc agccgcacgg tggcgctggc gctggtgatc ctgctcatca
     5941 ccttcggcgc ggcggtgacg gtctcggtgc gcaacaagca cgagcgccag cggcaggcgc
     6001 ggatgaccgc gctcttccag aacgccgaga gcctgcgcca gcgggccacc gagagcagcg
     6061 atccggcggc ggtgcagaac atcttgcagg cggccagcgc cagcatcacg cgcgccgagg
     6121 cgttcggtct cgaccaggat caagccgcgc gcgagcagac cgcgatcagc gccgaccgcg
     6181 accaggcgct caagatcgac cgcctggcga acatccaggt gctcggcagc ctgcccgccg
     6241 cgccgcgggg agtcacgccg cgcatcttcg ccggcaacgg ccagatctac gtcttcaccg
     6301 acgccctcta ccggctggat gccaacggca atgcgctggt gcgcctgatc ggcggcggcg
     6361 accagatcgg cggccagccg gtcgccaccc tgctcggcgc ggcctggggt gacggcgcgc
     6421 cggtcgtctt cgacggcagc cgcgcctacc tcttcgatcc gccgaccggt agctggcacg
     6481 cgcagcaggt ggagacgctg ggcggcccgg aaacgggcat cgccgcgatc aacggctaca
     6541 gcggcaacct ctatctcctc tcgccgcaga ccgggcagat cgtcaagtac ggcgccggcg
     6601 cctacgacaa ccagcccgag gactggaccg cgggcgccgc caccgacgac ctgaagcagg
     6661 cggtcgactt cgtcatcgac gggcacatct atgtgctgac caaggacggc cgggtgatcg
     6721 acttcttcat gagcgccgag cagtcgaccg tgaccccgca cgccgtgccg ccggtgacca
     6781 acgcggtggc gctctcactg cagaccgacc ggccctacgt ctacatcgcc gagcgcgacc
     6841 gtatcctgcg attggagcgc agctcgggca acctggtgca gcagttcgtg gccggcagcg
     6901 atgccccgcc cttcaacgac atccgcgcct ttagcgtcga cgacgtgctg ggcactgcct
     6961 acatcttgaa cggcaacgcg ctcctcagcg tgcgcctgcc cggcccgccg cactgatcta
     7021 cgacccgcct cgccatatac ctggaggggc agacctctgt gtctgccctc gtggccttat
     7081 tgccggcctt caccgacgcc acccgtgctc ccctattccc cgggggcata ggcgtagggg
     7141 gcacagtgca gacacggagg tctgcgcctg cagcgagaag gtcacggccg aatagaacac
     7201 atgttcggca tggtgcttgc gttgactgac cgggcgcgcg cggcgcaccg agtcagaaag
     7261 gagcaccgat gtcgttggaa caactgcgcg gcagcgcgat gatggagcat ctcgtggatg
     7321 ccctgggcca gggcaaggat atcggccact acggtaggct gacggtagcc atggtggggc
     7381 acttctacct cgacaagcac gaactgctgc agctgctgga gcagggccaa ggcgccagtg
     7441 aagaggactt gatggcgctg gtgcaggagg tggaggcgcg cgactacaac ccgccgacgc
     7501 gcgggcagat cctcgactgg cagcaacagc aggacttccc gatctgcccg catcctgagg
     7561 accccgacgc ctgcaacgtc taccaggagc tgaagctgcc cgagcgcgtc tacgcccaga
     7621 tcaaggagta ccgcgccgag cagttcgagc aggaggagca ggccgagcag cacggctgag
     7681 actgttgggt gcgacgtcaa gcaggggcga tatcgtcgag cgagacgacc acctcgtctc
     7741 cgtcgggcag ccgcacgcgc gcctcgttgc ggggaatact ggccgcgatg aattgcccca
     7801 cgccgccggg gaagcgaacc cggctgccga gcgtgggcgc ggcgcccagt cgctggagcc
     7861 ggtcgcgcac cagcgtggcc agctccggcg cctccggccg cagccggccc aggctgccga
     7921 ccagcggcgc gccggccggc tcgcgctcca gctcgacagg cacgccgagt agcgtggcga
     7981 gtcgtgcgcc gaaggcggcc agatcgagcg cgtcaggcgc ctgcagagag cagatggcgc
     8041 ggccgtcgag ggcgtagcgt aggccggtcg gtatgaggcc ggagaactca ttggccgcca
     8101 ggcgagcgac ctgtgccgtc tgctcgagcg cgcgctcggc ctggcgctcc acacgtgtga
     8161 cctcgtcgtc ctccagccgg cgcgtcacct cgggccaggg ttcggccagc ggcccggcct
     8221 cgacctcgcc cggcgcggcg gccacgcgcg ccggctcctc gccgtagagg cccggaaacg
     8281 cgacccagtc gccccgttgc agcgggagcg tggtgtgggc gaagcgggtc aggctcgacc
     8341 cggcgatgcg aagcgcgatg agctcgggga tcggagctac cggcgggctg gcttcggcca
     8401 ctgcgtcacc atgcggtcga gcgccaggcg cggctggacg ttgatgctga ggtcggtcag
     8461 cgcgtcgtag gtggcgtgca gcgcctgctg gatctcggga agcgaccagc gcagcgccag
     8521 cgcctcgatg cgcgcggcca cgtcggcatt gaccatctgc tcggcgcagc cgccggccgc
     8581 cagcaggatg tcgcgccaga ggcccagcag cgtgtcgagc tgtgccgcca cctcatcgcg
     8641 cttgccgcgc cggaaattat cggccagcga cctcgctgcc ttgagcgcct ccagcggatt
     8701 ctcgatcatc gccagggccg cattgatggc cttttcgcgc tcatctcgcg ccttcggatc
     8761 gtccgccagt gccagcgcct gccccagccg gccgcgcgtc agcgccgcga ggctggcggc
     8821 ctggtccggc tcgacgccgc gcgctcgtag ttgagcggcg acctcggcgc gcggcagcgg
     8881 ctccagcggg accggccggc aacgggagcg gatcgtctgc ggcacgcgct ccacctcggt
     8941 cgcgatcagc agcagcacga caaacggagg aggctcctcc agcgtcttga gcagcgcgtc
     9001 gaaggccggc cacgagaagc gctccacatc ctccagtagc gccacgcgcc agggcccctc
     9061 cagcggccgc agcgccagcg ccgtgcgcag ctcgcgcacc gactcgatcg agatctgcgt
     9121 gttcttggcg tcgcgcttgc tctcggcggc ctgggtctcc aggctgagcg tgagcacgtc
     9181 ggggtggagg ccgcgcgcta cccggctgca ggccgagcag acaccgcacg gcttgcgctc
     9241 ggcggtgcag aggagcgcct gggtgaaggc caccgccagc gtgtgccggc cgataccctc
     9301 cggcccgctg atgaggtagg cgtggccgac ttggccgccg cccacggcgc ggctgagcaa
     9361 tgccacggcg tccgcatgcc cgaccaccgg ccagctcccc gcctcggccc ctgtcagcgt
     9421 caccgtgcgc tcctaggcgg cacgcgcgcg cttcttggcg cccggttgcg gtccggtcgg
     9481 cagcgtctgc gggccctcga tgccctcgcc ctgggacagc gcctcgacga tctcgtcgcg
     9541 ctgctgcttg gtcagcatcg tgtcgccgct gaggtcgcgg atggcgttgt aaagctgatc
     9601 gagcgtcacc acggtgatgt ccgaggagat gacttcgagc tccagcgggc ggcgcggatt
     9661 cggcaggaag gtgatcacgc cctcgatatc gtcggcgccg gccaggttgt gcgtcgtcag
     9721 gaaatcggcc aggttcttct gctcgcgctc gctctcagcg gtggggttgc cgagctgcgg
     9781 cgaacccatg cccaccagga accagaggcg gccgccggca cggtggaagc ggttgtcctt
     9841 gaccttgacc tgcccgaaga catcgcgtgt cgtgatcacg atcaggccgg ccgggccgac
     9901 caggatgtgc tccgggtgac cgggcacggc gtcgctgtag tggatcagcg tgaagcggtc
     9961 gttgagccgg cgcagcgcgt tgtcgagcac ctgatcggcc gatggcttgc ggctccactt
    10021 ggtcacctgc tgcatgccgt actggaagcc gatgaagccg aggatcagca cgacgtaggc
    10081 gggcaggatc aggccggggg tgacgaaggt cagcaccagc gagaagagga gggccaggcc
    10141 gccgccccag gcgaccagtc gcccggcgcg ctgccgcgtc ttgatgtagc ccgtatttcg
    10201 cacgacgcgc atcgagggga tcatccgttc cagcggcgcc agggtcgcta ccagctagag
    10261 tctagcacgc gaccgccgga cgcgtcgaag ccgcgcacgc ggctagccgg acggcgcggc
    10321 gccgcgctcc tcgtcgagca gg
//
LOCUS       DASUBI010000847         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_128384, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000847 DASUBI010000000
VERSION     DASUBI010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128384"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VFU72_16825"
     CDS             <1..1409
                     /locus_tag="VFU72_16825"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU5425207.1"
                     /translation="AALVTLALWSFGPEIAAAAGRPLGHAWSRFGPVALAAAAAVLLL
                     VLSGAYTAARPAVECGGWPLCDGRVLPAGFDPTDIQLIHRWIALVASLAVLAAAVQAR
                     RLRADAPVLVATATAAAVLTAAQILVGAASVWFRLNPALLVAHLAVAIVVLMLLVAAV
                     ALDRLLPLAPVARPERRSWRLIGADYVTLTKPGVMVLLLITTLGAMLIAQGGLPPLRI
                     LFWTLIGGALSSGGAAAWNHYLDRDIDVIMKRTRSRPLPGKRIPDIHVPLFALTLTVL
                     SVYLLAVFVNPLAALLSLAGNLFYVLIYTRVLKRSTPQNIVIGGAAGAVPPLVGWAAV
                     TGQVGLPALLLFGVVFYWTPPHFWSLALFTSSDYAAASVPMFPEVYGDEETRRHIFLY
                     TVMLVITSLFFFAIRVMGLFYLASALILGGLFLYKAFQLTRHPADQKLLARALFFYSM
                     WYLGLIFAAMVIDRVLLG"
     gene            complement(1616..2143)
                     /gene="folK"
                     /locus_tag="VFU72_16830"
     CDS             complement(1616..2143)
                     /gene="folK"
                     /locus_tag="VFU72_16830"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5425208.1"
                     /translation="MARVAVALGSNLGDRAANLARAWQRLDAILSDARMSALYETDPV
                     GYLDQGRFLNAVVAGTTELGPAELLGALRRIEAELGRERPFPNSPRTLDLDLLFYDDL
                     ILETPELTLPHPRLHERFFVLVPLAELAPDLRHPVLGRTVRELLAALGPPQGIWPYRR
                     DDGACSSAWREGCPW"
     gene            complement(2143..2988)
                     /gene="folP"
                     /locus_tag="VFU72_16835"
     CDS             complement(2143..2988)
                     /gene="folP"
                     /locus_tag="VFU72_16835"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5425209.1"
                     /translation="MSALPATRCGARTLDWGRQTYVMAILNLTPDSFSGDGRLGREEE
                     ALAWALQAERDGADLLDIGGESTRPGHQPVAAAEELRRVVPVIAALAPRLAIPISIDS
                     SKAAVAEAALAAGASMVNDVRGLLGDPELAAVVARAGVPVVVMHDVAPEPGRDLVSSI
                     IRELDRRLDHALAAGIAWEQILLDPGFGFGKDWRQNLELLRRLSELRVLERPLLAGTS
                     RKRTIGRVLGLPVEERLEGTAATVALAIAGGADVVRVHDVRAMVRVARMSDAVVRGRL
                     PETEG"
     gene            complement(2985..>3295)
                     /locus_tag="VFU72_16840"
     CDS             complement(2985..>3295)
                     /locus_tag="VFU72_16840"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEU5425210.1"
                     /translation="AHIGYIPQGRVVGLSKLARLVDGYARRPQVQERLTSEIADALMD
                     ELHPNGCGVVIEAVHTCMTIRGIHKPGATMVTSAVRGGFRRRPATRAEFFALVGHPGR
                     "
BASE COUNT          405 a         1273 c         1105 g          512 t
ORIGIN      
        1 tggcggcgct ggtgacgctg gcgctttggt ccttcggccc ggagatcgcg gccgccgccg
       61 gtcgcccctt gggccacgcc tggagtcgct tcggcccggt cgcgctggcc gccgccgcgg
      121 ccgtgctgct gctggtgctc agcggcgcct acaccgccgc ccgcccggca gtggagtgcg
      181 gcggctggcc gctctgcgac gggcgcgtgc tcccggccgg cttcgacccg accgatatcc
      241 aactgatcca ccgctggatc gcgctggtcg cctcgctggc cgtactggca gccgccgtgc
      301 aggcccgccg cctgcgggcc gacgcgcccg tgctggtggc caccgccacc gccgccgccg
      361 tgctgaccgc cgcgcagatc ctggtcggcg cggccagcgt ctggttccgg ttgaaccccg
      421 ctctgctggt ggcgcacctg gcggtcgcca tagtcgtgct catgctgctg gtagccgccg
      481 tcgcgctcga ccggctgctg ccgctggcgc cggtcgcgcg accggagcgg cgctcctggc
      541 gcctgatcgg cgcggattat gtcacgctga ccaagccggg cgtgatggtg ctcctcctga
      601 tcaccacgct cggcgccatg ctgatcgccc agggcgggct gccgccgctg cgtatcctct
      661 tctggacgct gatcggcggc gcgctctcct ccggcggcgc ggcggcctgg aaccactacc
      721 tcgaccgcga catcgacgtc atcatgaagc gcacccgcag ccgaccgctc cccggcaagc
      781 gcatcccgga tatccacgtg ccgctcttcg ccctgacgct gacggtgctc tcggtctacc
      841 tgctggccgt cttcgtcaac ccgctggcgg cgctcctctc gctggccggc aacctcttct
      901 acgtcctgat ctacacccgg gtgctcaagc gcagcacgcc gcagaacatc gtgatcggcg
      961 gcgcggccgg cgcggtgccg ccgctggtcg gctgggcggc cgtcaccggc caggtcgggc
     1021 tgccggcgct cctgctcttc ggcgtcgtct tctactggac gccgccgcac ttctggtcgc
     1081 tggcgctctt cacctcgtcg gactacgccg ccgccagcgt gccgatgttc cccgaggtct
     1141 acggcgacga ggagacgcgg cgccacatct tcctctacac cgtgatgctg gtgatcacct
     1201 ccctcttctt cttcgccatc cgggtcatgg ggctgttcta tttggcgagc gcgctgatcc
     1261 tcggcgggct cttcctctat aaggcgtttc agctcacgcg ccacccggcg gatcaaaagc
     1321 tgctggcgcg cgccctcttc ttctactcga tgtggtacct cggcctgatc ttcgccgcca
     1381 tggtcatcga ccgcgtcctg ctgggctagc gctcgaccgc agcagcccgc gcggcgtcga
     1441 agctcccagc gcttcgcgct gccctgggtt ggtgccatgc gttgcccgcc tgtagcggca
     1501 gacccctgtg tctgccctcg tcgccctggc actcgccgtc accgacgcaa tcgcagctac
     1561 cctatccccc gttgccatga cgcaggggta acagggcaga cacaggggtc tgcccctacc
     1621 acggacaccc ctcgcgccaa gcgctcgaac atgccccgtc gtcgcgacgg tagggccaga
     1681 tgccctgggg cgggcccagc gccgccagca gctcgcgcac ggtccggccg agcaccgggt
     1741 gccggaggtc tggggccagc tcggccagcg gcaccagcac gaagaagcgc tcgtgcagcc
     1801 gcgggtgcgg cagcgtgagc tcgggcgtct ccagaatgag gtcgtcgtag aagagcaggt
     1861 cgaggtcgag ggtgcgcggg ctgttgggga acggccgctc acgccccagc tccgcctcga
     1921 tgcgccgcag ggcgccaagg agctcggccg ggccgagttc ggtcgtcccg gccacgaccg
     1981 cgttgaggaa gcgcccctgg tccaggtagc cgaccggatc cgtctcgtag agcgccgaca
     2041 tgcgcgcatc gctgaggatc gcgtcgaggc gttgccaagc acgcgccaga ttcgcggcgc
     2101 ggtcgcccag gttgctgccc agcgcgacgg ccacgcgggc cattagccct ccgtctccgg
     2161 taaccggccc cgcaccaccg cgtcgctcat gcgcgccacg cggaccatcg cgcgcacgtc
     2221 gtgcacccgc accacgtcgg ccccgccggc gatcgccaag gccaccgtgg cggcggtccc
     2281 ctccagccgc tcctccaccg gcaggccgag cacgcggccg atggtgcgct tgcgcgaggt
     2341 gccggccagg agcgggcgct cgagcacccg cagctcggac agccgccgta gcagctccag
     2401 gttctggcgc cagtccttgc cgaagccgaa gcccgggtcg agcaggatct gctcccaggc
     2461 gatccccgcc gccagcgcgt gatccagccg ccggtccagc tcgcggatga tgctgctgac
     2521 gaggtcgcgc cccggctccg gcgccacgtc gtgcatgacc acgaccggca ccccggcccg
     2581 cgccaccacc gccgccagct ccgggtcgcc gagcaggcca cgaacgtcgt tgaccatgct
     2641 ggcgcccgcg gccagcgccg cctcggccac cgccgccttg ctgctgtcga tcgagatggg
     2701 gatcgccagc cgcggggcca gcgccgcgat cactggcacg acgcggcgca gctcctccgc
     2761 cgccgcgacc ggctggtgcc cgggccgggt cgactccccg ccgatgtcga gcaggtccgc
     2821 gccgtcccgt tcggcctgca gcgcccaggc cagcgcctcc tcctcgcggc ccagccgtcc
     2881 gtcgccgctg aaggaatccg gcgtcaggtt gaggatcgcc atgacgtagg tctgccggcc
     2941 ccagtcgagc gtgcgcgcgc cgcagcgcgt cgcggggagc gcgctcatcg tcccggatga
     3001 ccgacgaggg cgaagaactc ggcgcgcgtg gccgggcggc gacggaagcc gccgcgcacc
     3061 gccgaggtga ccatcgtcgc ccccggcttg tgaatgcccc gaatcgtcat gcaggtgtgg
     3121 accgcttcga tcaccacgcc gcagccgttg ggatggagct cgtccatcag cgcgtcggcg
     3181 atttcgctcg tcagtcgctc ttgcacctgc ggccggcggg catagccatc gaccagccgc
     3241 gccagcttgc tcaaacccac gacgcggcct tgcgggatgt agccgatgtg ggcgt
//
LOCUS       DASUBI010000848         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_362387, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000848 DASUBI010000000
VERSION     DASUBI010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362387"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3073)
                     /gene="rpoB"
                     /locus_tag="VFU72_16845"
     CDS             complement(<1..>3073)
                     /gene="rpoB"
                     /locus_tag="VFU72_16845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5425211.1"
                     /translation="QQRVAAAEAEGRTDLSSPSGLEEIFEEISPIEDFSGTMSLSFRD
                     HRFEPAKYSIDECKDRDFTYAAPLFVTAEFTNNETGEIKSQTVFMGDFPLMTNKGTFI
                     INGTERVVVTQLVRSPGLYFSQDIDKTSDKDVFNAKIIPSRGAWLEFEIDKRDMVGVR
                     VDRKRKQHVTVLLKALGWDDARILEEFGEYESMRQTLEKDHTAGQDEALLDIYRKLRP
                     GEPPTREAAQTLLDNLYFNPKRYDLAKVGRYKIDKKLGQASGIDNTVLTEDDIVNAIR
                     FLVKLHAGETEMPPAAEGMPNVVVEEDDIDHFGNRRLRSVGELIQNQVRTGLARMERV
                     VRERMTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNPLSGLTHKRRLNA
                     LGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLASYGRINAFGFIETP
                     YRKVTDGRVTDHIDYLTADEEDRYVVAQANAPVRDDGSYADDRVLCRRKGGEIEMMAP
                     ADIDYMDVSPRQMVSVATAMIPFLEHDDANRALMGANMMRQAVPLLQSDSPYVGTGME
                     YRCAVDAADVVTADKAGVITEVSADLVSVANDDATTSVYRIAKFRRSNQGTSFNQKVI
                     VAEGERVEVGSVLADGPCTQDGEMALGKNLLVAFMPWEGHNYEDAIILSQRLVQDDVL
                     SSIHIEEHEVDARDTKLGPEEITRDIPNVSEEVLADLDERGIIRIGADVVPGDILVGK
                     VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGEQGKIIGVRIFDRDSGDELPP
                     GVNQLVRVYVAQKRKITHGDKLAGRHGNKGVIAKILPVEDMPFLDDGTPVDIVLNPLG
                     VPSRMNPGQVLETHLGWIAATGWDIGDGQAEWQERLRSIGADKAEARTKVATPVFDGA
                     REDEIAGLLNSTLKTRDGVRLVNESGKARLFDGRSGEPFPYPISVGYMYILKLHHLVD
                     DKLHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLT"
BASE COUNT          409 a         1024 c         1077 g          563 t
ORIGIN      
        1 tggtcagcag ttcctgcagc gcgtaggcgg ctccgtacgc ctcgagcgcc cacacctcca
       61 tctcgccgaa ccgctggccg ccgaactggg ccttaccgcc cagcggctgc tgcgtgatca
      121 tcgagtaggg gccggtgctg cgcgcgtgca gcttgtcgtc caccaggtgg tgcagcttca
      181 ggatgtacat gtagccgacc gagatcgggt acgggaacgg ctcgccggag cggccgtcga
      241 acagccgcgc cttgccggac tcgttgacca gccgcacgcc gtcgcgggtc ttcagggtgc
      301 tgttcagcag gccggcgatc tcgtcctcgc gggcgccgtc gaacaccggg gtcgcgacct
      361 tggtgcgcgc ctcggccttg tccgcgccga tcgagcgcag gcgctcctgc cactcggcct
      421 gtccgtcgcc gatgtcccag ccggtggccg cgatccaccc gaggtgggtc tccagcacct
      481 ggccggggtt catccgggag ggcacgccca gcgggttcag cacgatgtcg accggggtgc
      541 cgtcgtccag gaacggcatg tcctccaccg ggaggatctt ggcgatgacg cccttgttgc
      601 cgtgccggcc ggccagcttg tcgccgtgag tgatcttgcg cttctgcgcc acgtacaccc
      661 ggaccagctg gttcacgccc gggggcagct cgtcgccgct gtcccggtcg aagatccgga
      721 cgccgatgat cttgccctgc tcgccgtgcg gcaccttcag cgaggtgtcg cgcacctcgc
      781 gggccttctc accgaagatc gcccgcagca gccgctcctc cggggtgagc tcggtctcgc
      841 ccttgggcgt gaccttgccg accaggatgt cgccggggac gacgtcggcg ccgatccgga
      901 tgatgccgcg ctcgtcgagg tcggccagga cctcctcgga gacgttcggg atgtcccggg
      961 tgatctcctc cgggcccagc ttggtgtccc gggcgtcgac ctcgtgctcc tcgatgtgga
     1021 tcgaggacag gacgtcgtcc tgcaccaggc gctgcgacag gatgatcgcg tcctcgtagt
     1081 tgtggccctc ccacggcatg aacgccacga gcaggttctt gcccagggcc atctcgccgt
     1141 cctgggtgca cgggccgtcg gccagcaccg agccgacctc gacccgctcg ccctcggcca
     1201 cgatcacctt ctggttgaag gaggtgccct ggttggagcg gcggaacttg gcgatccggt
     1261 agacgctggt ggtggcgtcg tcgttggcga cgctcaccag gtcggcggag acctcggtga
     1321 tgacgccggc cttgtcggcg gtgaccacgt cggcggcgtc gaccgcgcag cggtactcca
     1381 tgccggtgcc gacgtacggc gagtccgact gcagcagcgg cacggcctgg cgcatcatgt
     1441 tcgcgcccat cagggcgcgg ttggcgtcgt cgtgctccag gaacgggatc atcgcggtgg
     1501 cgaccgacac catctggcgc ggggagacgt ccatgtagtc gatgtcggcc ggcgccatca
     1561 tctcgatctc gccgcccttg cggcggcaca gcacgcggtc gtcggcgtag gagccgtcgt
     1621 cccggaccgg cgcgttcgcc tgcgcgacga cgtagcggtc ctcctcgtcg gccgtcaggt
     1681 agtcgatgtg gtcggtgacc cggccgtcgg tgaccttccg gtacggggtc tcgatgaagc
     1741 cgaaggcgtt gatccggccg taggaggcca gcgagccgat caggccgatg ttcgggcctt
     1801 ccggcgtctc gatcgggcac atccggccgt agtgcgaggg gtgcacgtcg cgcacctcga
     1861 agccggcccg ctcgcgggac agaccgccgg ggcccagggc gttcagccgg cgcttgtggg
     1921 tcagaccgga cagcgggttg gtctggtcca tgaactgcga gagctggctg gtgccgaaga
     1981 actccttgat ggaggccacg accggccgga tgttgatcag cgtctgcggg gtgatggcct
     2041 cgacgtcctg cgtggtcatg cgctcgcgga cgacccgctc catgcgggca aggcccgtgc
     2101 ggacctggtt ctggatcagc tcgccgacgc tgcgcagccg gcggttgccg aagtggtcga
     2161 tgtcgtcctc ctcgacgacc acgttcggca tgccctcggc ggccggcggc atctcggtct
     2221 cgccggcgtg cagcttgacc aggaagcgga tggcgttgac gatgtcgtcc tcggtcagca
     2281 ccgtgttgtc gatgccgctg gcctgcccca gcttcttgtc gatcttgtag cggccgacct
     2341 tggccaggtc gtagcgcttg gggttgaagt acaggttgtc cagcagggtc tgggcggcct
     2401 cacgcgtcgg cggctcgccc gggcgcagct tgcggtagat gtccagcagc gcctcgtcct
     2461 ggccggcggt gtggtccttc tccagcgtct ggcgcatcga ctcgtactcg ccgaactcct
     2521 ccaggatccg ggcgtcgtcc cagcccaggg ccttcaggag cacggtgacg tgctgcttgc
     2581 gcttgcggtc cacgcgcacg ccgaccatgt cgcgcttgtc gatctcgaac tccagccagg
     2641 cgccccggct ggggatgatc ttggcgttga agacgtcctt gtcggaggtc ttgtcgatgt
     2701 cctgggagaa gtacagaccc ggggagcgca ccagctgggt caccacgaca cgctcggtgc
     2761 cgttgatgat gaaggtgccc ttgttggtca tgagcgggaa gtcgcccatg aacacggtct
     2821 ggctcttgat ctcgccggtc tcgttgttcg tgaactcggc ggtcacgaac agcggggcgg
     2881 cgtaggtgaa gtcgcggtcc ttgcactcgt cgatggagta cttcgcgggc tcgaagcggt
     2941 ggtcacggaa cgacagcgac atcgtgccgc tgaagtcctc gatcggggag atctcctcga
     3001 agatctcctc gaggcccgac ggggacgaca gatcggtgcg gccttccgct tcggcggcgg
     3061 ccacccgctg ctg
//
LOCUS       DASUBI010000849         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_1125690, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000849 DASUBI010000000
VERSION     DASUBI010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125690"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1504)
                     /locus_tag="VFU72_16850"
     CDS             complement(332..1504)
                     /locus_tag="VFU72_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5425212.1"
                     /translation="MSGLGGGFSLGKIFGIEIRIDWSWILIFLLITWNVYIVLPQLND
                     TWSRPFTVFLAIIASILFFLSVLAHELAHSLMAKARGINVSSITLFLFGGVSNITREP
                     PTPGAEFAIAIVGPLTSIVLGFIFILLANVSYAAVAHPLRDLNHVDPATAIFLWLGPV
                     NLILGIFNLVPGFPLDGGRVLRSILWAITDNLKKATRWASWIGDAIGWLFIVMGVYMV
                     FGGTVPFFGSGLGGLWIAFIGWFLHNAAQASYQQVVITDILKGVPVTRIMRTNVPTVP
                     ADAHVSQLVDQIMQSDERSFPVMRGDELVGLITLDDVRRVPRDQWETTPVRQIMTPVD
                     KLAVASPQEDANDVFTELATKDVNQLPVVQDGRLVGMVRRGDLLRFLQLHQEGVRA"
     gene            complement(1718..>2117)
                     /locus_tag="VFU72_16855"
     CDS             complement(1718..>2117)
                     /locus_tag="VFU72_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5425213.1"
                     /translation="ARAERYLERAVEVALARAPASAANCVATLAYFQAYRGRQMEACR
                     EALDLLRAFGLKRDVLRRLEAAISLAEGDRAFGRAQARAALDELDKSWLTGLARADRD
                     WLEHLLAESEEASASAPAALRSLRERTIAA"
BASE COUNT          368 a          689 c          764 g          296 t
ORIGIN      
        1 gcggccgagc tggtaggcat tcagcagcgc ggccagcgcc aggatgccgc ccagcgcggc
       61 gtgctgccag ccgagcgcca gcgccacgcg ccgcacgtcc ggccgggcgg cttcgcccgc
      121 ctgctcggga agcgcctcga cgttcatact cctccatcac ggcttctgcc gctctccgca
      181 agggaggata gagcgcgcgc ctgaaaaggt gctgagaaga ggatgagagt tttctcagcc
      241 ggggcgggcc atggcggacg aatggtggcg gacgaggcgc atgggatccg tgccccacgc
      301 ggcagccaac atgctcgaag gccaccgccc gtcaggcccg cacgccctcc tgatgcaatt
      361 gcaggaagcg gagcaggtcg ccgcggcgca ccatgccgac cagccggcca tcctgcacca
      421 cggggagctg gttgacatcc ttggtcgcca gctcggtgaa gacgtcgttg gcgtcctcct
      481 gcggcgaggc gacggccagc ttgtccaccg gcgtcatgat ctggcgcacc ggcgtcgtct
      541 cccattggtc gcgcggcacc cggcgcacgt cgtcgagcgt gatcaggccg accagctcgt
      601 cgccgcgcat caccgggaag gagcgctcgt cgctctgcat gatctggtcg acgagctggc
      661 tgacgtgggc atccgccggc acggtcggga cgttggtgcg catgatccgc gtcaccggca
      721 cccccttgag gatgtcggtg atgacgacct gctggtagct ggcctgggcg gcgttgtgca
      781 ggaaccaacc gatgaaggcg atccagaggc cgcccagacc ggagccgaag aaggggaccg
      841 tgccgccgaa gaccatgtag acacccatga cgatgaagag ccagccgatg gcgtcgccga
      901 tccaggaggc ccagcgcgtg gctttcttga gattatcggt gatcgcccaa aggatcgagc
      961 gcagcacccg gccgccgtcg agcgggaagc cggggacgag gttgaagatg ccgaggatca
     1021 ggttgaccgg gccgagccag aggaagatgg cggtggcggg gtcgacgtgg ttcagatcgc
     1081 gcagcgggtg ggcgaccgcc gcgtagctga cattggccag caggatgaag atgaagccga
     1141 gcacaatgct ggtgagcggg ccgacgatgg cgatggcgaa ctcggcgccc ggcgtgggcg
     1201 gctcgcgcgt gatgttcgag acgccgccga agagaaagag ggtgatgctg ctgacgttga
     1261 tcccccgggc cttggccatc agcgagtgag ccagctcgtg cgccagcacg gagaggaaga
     1321 agaggatcga ggcgatgatc gccaggaaga cggtgaaggg ccggctccag gtgtcgttga
     1381 gctgcggcag gacgatgtag acgttccagg tgatcaggag gaagatgagg atccagctcc
     1441 agtcgatgcg gatctcgatg ccgaagatct tgccgagcga gaaaccgccg ccgagtccag
     1501 acatgtcggc ctccttctcc ggcgcggagc gtgccacgcg aggatgccgg tctccgggcg
     1561 cgcgaccaat gatcgcacgc ttcactcggc attgtcgatg acggctaagg ccagagacag
     1621 ggcggcccga cggccggaat gcccgaccca ggggctggct cctgtgccaa accaggccag
     1681 ggaggcatcc gagcgcactc ggcagccgtc cgcttcctca ggcggcgatg gtccgctctc
     1741 ttaaggaccg gagcgcggcg ggagcggagg cgctcgcctc ctccgactcg gccaggagat
     1801 gctccagcca gtcgcggtcg gcgcgcgcca gtccagtgag ccaggacttg tccagctcgt
     1861 cgagggcggc ccgcgcctgc gcccggccaa aggcgcggtc gccctctgcc aggctgatgg
     1921 ccgcttccag ccgccgcagc acatcgcgct tgagcccgaa cgcgcgcagc agatcgagcg
     1981 cctcgcgaca ggcctccatc tgccggccgc ggtaagcctg gaagtaggcg agcgtcgcga
     2041 cacagttggc ggcgctcgcg ggggcccggg ccagcgccac ctccaccgcc cgttcgaggt
     2101 agcgctcggc ccgcgcc
//
LOCUS       DASUBI010000850         6864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrolancea sp. isolate SMAG_U2517 k141_237063, whole
            genome shotgun sequence.
ACCESSION   DASUBI010000850 DASUBI010000000
VERSION     DASUBI010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960974
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrolancea sp. (soil metagenome)
  ORGANISM  Nitrolancea sp.
            Bacteria; Thermomicrobiota; Thermomicrobia; Sphaerobacterales;
            Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:05:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,380
            CDSs (total)                      :: 3,344
            Genes (coding)                    :: 3,324
            CDSs (with protein)               :: 3,324
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Nitrolancea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237063"
                     /isolate="SMAG_U2517"
                     /isolation_source="soil"
                     /db_xref="taxon:1966479"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFU72_16860"
     CDS             <1..384
                     /locus_tag="VFU72_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5425214.1"
                     /translation="AADANTSVIAANAHWNGTLRSEGSVHIHGKADGELHSNHDVFVA
                     EGAEVDAQLFADNVIVAGVVRGKIEARGRLELLPQGHVSGDVRAPKLIVHEGARLSGQ
                     LKMETGNAVMTTEPPAAPKPRRSSQ"
     gene            400..957
                     /locus_tag="VFU72_16865"
     CDS             400..957
                     /locus_tag="VFU72_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5425215.1"
                     /translation="MAASEEAQMTYRPAGPPSPSPSSSPTGFGEGLPQHTAAPEAFSL
                     VDRYSTVEGTFTTKRDLRIEGEMRGTLRCQGLLFVAEGADVDATVEAANIAVAGQLRG
                     NVTCHGKLQIMPTGRVVASVVTETLVINEGAIYEGELRMEGSGQLSASSGGVPQVEEG
                     NPMLRRFGGEEETGLPAPRRARGEQ"
     gene            1421..2095
                     /locus_tag="VFU72_16870"
     CDS             1421..2095
                     /locus_tag="VFU72_16870"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5425216.1"
                     /translation="MGQYPPSGTRRPFGDDEDDEDLDPRDRYGATGRMGGTGGGTGGG
                     GGGGGPERQFNYNPDERYWTDYIRIALPVLGVIVLLALVWFWVNNLLGNDNKNNVAGT
                     TASPTTISIPNINLGSPTATSAAGVPPTHIAGTATSSTGAGATPPASATLKAGGKAVV
                     SAGGTGVNMRASPNTTAEIVAKLADGTDVTITGDSKAGENYTWWPVKVGDKTGWIAGD
                     FLKPAA"
     gene            2449..3294
                     /gene="rpsB"
                     /locus_tag="VFU72_16875"
     CDS             2449..3294
                     /gene="rpsB"
                     /locus_tag="VFU72_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5425217.1"
                     /translation="MATTTQMQDNETARATMKALLEAGVHFGHQTKRWNPKMRPFIFV
                     ERHGVHIIDLGQTIQLLNEAEDFAREVAANGGRIIFVGTKKQAQDAIRNEAGRSGMPF
                     VDRRWLGGTLTNFVTIRGRLRDLRDLEQRVHGGEFDNLPKQEQIRIQRELEKLQMSLG
                     GLRDLSQLPQAIFVVDPKREAIAVAEARRLGIPVIAMVDTNCDPDLVDYVIPANDDAI
                     RSVRLIASRLADAAIEGRTRLESAFSGEPGVDENAMSSYDLDEEYTPDEDEDEDSDSQ
                     SAARR"
     gene            3366..3932
                     /locus_tag="VFU72_16880"
     CDS             3366..3932
                     /locus_tag="VFU72_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU5425218.1"
                     /translation="MEITTQMVKDLRERTGAGVMDAKRALVASDGDMTKAAAIIEEQG
                     RNRAVRKEGRETSQGLVWSYIHGGQPGQNGGRVGALVEINCETDFVAKTDDFRDLCRD
                     VAMQVAAMDPKYISAGDIPETELRAQAASAEKYAERVALLSQPFIKDASKTIGELVKD
                     RIGKLGENIMVRRFARFELGVAPDIAEE"
     gene            3942..4685
                     /gene="pyrH"
                     /locus_tag="VFU72_16885"
     CDS             3942..4685
                     /gene="pyrH"
                     /locus_tag="VFU72_16885"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5425219.1"
                     /translation="MSEKAAARTCTRHPYRRVVLKLSGEALMGDAGYGIDPAVATRVA
                     QQIERVVADGVEIAIVVGGGNIWRGLAASARGMDRATADYMGMLATVINALALQDALE
                     HLGVHTRVQTAVEMHQVAEPYIRRRAIRHMEKGRVVILAGGTGNPYFTTDTAAALRAL
                     EIGAEVILMGKNNVDGVYDADPNVYPEARRYATLTFQEALERNLRVMDQAALALCREN
                     DLPLIVFDLLQPGNIERAVLGQEIGSLVC"
     gene            4700..5269
                     /gene="frr"
                     /locus_tag="VFU72_16890"
     CDS             4700..5269
                     /gene="frr"
                     /locus_tag="VFU72_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5425220.1"
                     /translation="MSDDMLREILADADDRMKKTIDALRRELSGIRTGRAAPGLIERL
                     DVDYYGTSTPLNQLAGISAPEARLLVVQPWDRGSMGAIEKAIRSSDLGLNPTNDGQLI
                     RIAIPALTEERRKSLVRVVRQKVEEDKVALRNVRRDAMADIKELQKEKLIGEDEERRA
                     EHELQEVTNRFIGEADKIGKHKEDEVLEV"
     gene            5250..6026
                     /gene="uppS"
                     /locus_tag="VFU72_16895"
     CDS             5250..6026
                     /gene="uppS"
                     /locus_tag="VFU72_16895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5425221.1"
                     /translation="MRSSKSEPAASQPDTCPPAPARVPRHVAIVMDGNGRWATQRGLP
                     RVAGHEAGTENIRRITRRAAERGIDYLTLWGFSTENWRRPQDEVEAILGILARSVSNE
                     TEELHHEGVRLKHIGSLAPLDPSLRYAIEDAIELTRNNTRVTLTIAFNYGGRAELIQA
                     IQRIVADGIAPEQIDEALISRYLYTHDMPEPDLIIRTSGELRVSNFLIWQGAYAEYIF
                     TSKLWPDFGPDDLVCAVEEYARRERRFGGLGPSAAPPGAA"
     gene            6040..>6864
                     /locus_tag="VFU72_16900"
     CDS             6040..>6864
                     /locus_tag="VFU72_16900"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5425222.1"
                     /translation="MLRQRSISAVVVVLFAAIPALLGGPLFAAAMVVLALLGTRELLR
                     ALDGRIDRATEALVYLAALGLILTAAFDGAGPGLAAVIVLFVIASLIAGVLRTAVETG
                     LAAWSAGILTIAYLGLPLAYAVALRDLPGASERGWVNHVTDPLAAGTGAGLAWVALVF
                     AVTWLTDTAAYLVGRRFGRTKLVPALSPGKTRAGALGGIVAGALSAPLAAWVFGAPLG
                     LPLAAVLGLILSLAGQLGDLGESLIKRNLGIKDMGTLIPGHGGVLDRIDALLFTFPL"
BASE COUNT         1171 a         2397 c         2310 g          986 t
ORIGIN      
        1 gcagccgacg ccaataccag cgtcatcgcc gccaatgccc actggaacgg gacgctgcgt
       61 agcgagggct cggtccacat tcacggcaag gccgacggtg agttgcacag caaccacgat
      121 gtcttcgtgg ccgagggcgc cgaggtcgac gcgcagctct tcgccgacaa cgtgatcgtg
      181 gccggcgtcg tccggggcaa gatcgaggca cgaggccggc tggagctgct gccgcaaggg
      241 cacgtctccg gcgacgtgcg cgcgccgaaa ttgatcgtgc acgagggcgc gcggctctcg
      301 gggcagctca agatggagac tggcaacgcg gtgatgacga ccgagccgcc cgcggcgccc
      361 aagccgcggc ggtcgtcgca gtaggaggaa ggccgccgcg tggcggcgtc cgaggaggcg
      421 cagatgacct accggccggc aggcccaccc tcaccctcac cctcatcctc tcccacaggc
      481 tttggcgagg ggctgccgca gcacaccgcg gcccccgagg ccttcagcct ggtcgaccgc
      541 tactcgacgg tcgaaggcac cttcacgacc aaacgcgatc tgcgcatcga gggcgagatg
      601 cgcggcacgc tccgctgcca gggattgctc ttcgtcgcgg agggcgcgga tgtcgatgcc
      661 acggtcgagg cggccaacat cgccgtggcc ggccagctgc gcggcaatgt gacctgccac
      721 ggcaagctgc agatcatgcc gaccggccgg gtggtcgcca gcgtggtcac cgagacactg
      781 gtcatcaacg aaggcgcgat ctacgagggg gagctgcgca tggagggctc cggccagctc
      841 agcgccagtt ccggcggtgt gccgcaggtg gaagaaggca acccgatgct gcgccgcttc
      901 ggcggcgagg aggagacggg cctgcccgcg ccgcgccgcg cgcgcggcga gcagtaacca
      961 ttccgcgacg gttcctccgc cgaaacacca tgctataatg gcccggacgg ctggggtaat
     1021 cgagcacgcg atcccaaggg acgttttgag tctgctatca ttgctgcccg atccgagccg
     1081 cagcaagcag accagggcgt ggcagagagg gaccggcgtg accgtccgga agtgcagcac
     1141 ctgcaagtat tacgagccgg cgccgatctg gcgcaagggc tggtgtcgga acccgcttct
     1201 ctattctccc cagcaaagcc atctcgtggg cgaggatgac ctcgactgcg accgcgggat
     1261 gggaaactat tgggaaccaa tcgacggcgc cgcggccgac gcgatggacg cgcccgacgc
     1321 ccagtccgag tcagagggcg ccgcgtcggt ggtcgtggca cctgtgcagt tcgctgggct
     1381 ccggcgcaag gccggcgccc agggtgacgg aggagatgac atgggccagt accccccgtc
     1441 tggaacccgg cgtccgttcg gcgacgacga ggacgacgaa gacctcgacc cgcgcgaccg
     1501 gtacggggcc actgggcgga tgggcggcac cggcggcggc actggtggcg gtggcggtgg
     1561 cggcggcccc gagcgccagt tcaactacaa cccggacgag cgctactgga ccgactacat
     1621 ccgcatcgcg ctccccgtgc tcggggtgat cgtcctcctc gcgctcgtct ggttctgggt
     1681 caacaacctc ctcggcaacg acaacaagaa caacgtcgcc ggcaccaccg catcaccaac
     1741 caccatcagc atcccgaaca ttaacctcgg cagcccgacc gccaccagcg cggccggcgt
     1801 accgccgacg cacatcgccg gcacggccac gtccagcacc ggcgccggtg ctacgccgcc
     1861 agcctcggca acgctgaagg cgggcggaaa ggcggtcgtg agtgccgggg gcaccggagt
     1921 caacatgcgc gccagcccca acaccaccgc cgagatcgtg gccaagctcg ccgacgggac
     1981 tgacgtcacc atcaccggcg actccaaggc gggcgagaac tacacctggt ggccggtcaa
     2041 ggtcggcgac aagaccggct ggatcgcggg agacttcctc aagcccgcgg cctaagccac
     2101 agcgcgccgc tttagcacgc ggcaccgttc cagtcgggac ggggccgcgt gttcgtgaag
     2161 gcgagtgccg cgcgacgccc gagaatcaaa ttctcgggcc gagctttcac cgttcaccga
     2221 tggcgacgac actctccccc gcgcgaaccc cccaaggctg cccgaaccct tgccagcctg
     2281 tccaacatgc cccccggccc ggcgccagcc gcctatgcta tactccttga ggcaactttc
     2341 gttgccaaat tttcacgtgc ccagaccgtg gctggggtgc cgtgtgcgca cggtccagcg
     2401 atggggtgaa gggcacggac gtgcaacaac cctggagagg aacacacctt ggctacgaca
     2461 acccaaatgc aggacaacga aaccgctcgt gccacgatga aggcgctgct ggaggccggc
     2521 gtccacttcg gtcatcagac caagcgctgg aatcccaaga tgcgtccctt catcttcgtc
     2581 gagcggcacg gcgtccacat catcgacctc ggccagacga tccagctcct caacgaggcg
     2641 gaggacttcg cccgcgaggt cgccgccaac ggtggccgta tcatcttcgt cggcaccaag
     2701 aagcaggcgc aggatgcgat ccgcaatgag gccggtcgct ccggcatgcc attcgtcgat
     2761 cgccgctggc tgggcggcac cctgacgaac ttcgtcacga ttcgtggccg gctgcgcgac
     2821 ctgcgcgatc tggagcagcg ggtccacggc ggcgagttcg ataacctgcc caagcaggag
     2881 cagatccgca tccagcgcga gctggagaag ctgcagatgt cgctcggcgg cctgcgtgat
     2941 ctgagtcaac tgccacaggc gatcttcgtg gtcgatccca agcgcgaggc gatcgcggtc
     3001 gccgaggcgc ggcggctcgg catcccggtc atcgccatgg tcgataccaa ctgcgacccc
     3061 gatctggtcg actacgtcat cccggccaac gacgacgcca tccggtccgt gcgcctgatc
     3121 gcctcccggc tggccgacgc ggccatcgag ggccgcactc gacttgagag cgccttttcc
     3181 ggcgagccgg gcgtcgacga gaacgctatg agctcctacg atctcgatga ggagtacacc
     3241 cccgacgagg acgaggacga ggacagcgac agtcagtccg cggcgcggcg ctaggctgtc
     3301 agaaccgggc cggccggcgg ctggcccagg tccgtctcca gcatactgat gacgagggag
     3361 gaagcgtgga gatcaccacc caaatggtga aagatttgcg tgagcgcacg ggcgctggtg
     3421 tcatggacgc caagcgggcg ctcgtggcca gcgacggcga catgacgaag gccgccgcga
     3481 tcatcgagga gcagggccgc aaccgggccg tgcgcaaaga gggccgcgag acctcgcagg
     3541 gtctcgtctg gtcctacatc cacggtggcc agccgggcca gaacggcggc cgggtcggcg
     3601 cactcgtcga gatcaactgc gagaccgact ttgtcgccaa gaccgatgac ttccgcgacc
     3661 tctgccgcga tgttgcgatg caggtcgccg cgatggatcc gaagtacatc tccgccggcg
     3721 acattcccga gacggagctg cgcgcgcagg ccgccagcgc cgagaagtac gccgagcggg
     3781 tggcgctgct gtcgcagccc ttcatcaagg acgcctccaa gacgatcggc gaactggtca
     3841 aggaccgtat cggcaagctg ggcgagaaca tcatggtgcg tcgcttcgct cgcttcgagc
     3901 tcggcgtcgc gcccgatatc gccgaagagt agaggctcgg ggtgagcgag aaagccgccg
     3961 cacggacctg cacccgtcac ccctatcgcc gggtcgtgct taagctctcc ggcgaggccc
     4021 tgatggggga cgccggctac ggtatcgacc cggccgtggc cacgcgggtg gcccagcaga
     4081 tcgagcgcgt ggtcgccgac ggcgtcgaga tcgccatcgt cgtgggcggt ggcaacatct
     4141 ggcgcgggct ggccgccagc gcgcgcggca tggatcgcgc caccgccgac tatatgggca
     4201 tgcttgccac cgtgatcaac gcgctggcgc tgcaagacgc gctcgagcac ctgggtgtac
     4261 acacccgggt gcagaccgcg gtcgagatgc accaggtggc cgagccgtac atccggcgcc
     4321 gcgcgatccg gcacatggag aaggggcgcg tcgtgatcct ggccggcggc accggcaatc
     4381 cctacttcac caccgacacg gccgccgccc tgcgcgccct ggagatcggc gccgaggtca
     4441 ttctgatggg caagaacaac gtcgacggtg tctacgacgc cgatcccaac gtctaccccg
     4501 aggcccggcg ctacgccacc ctgacctttc aggaggcgct cgagcgcaac ctgcgcgtga
     4561 tggaccaggc ggcgctggcc ctctgccgcg agaatgacct gccgctgatc gtcttcgatc
     4621 tcctgcagcc ggggaacatc gagcgagccg tgctcggtca ggagatcggc agcctcgtct
     4681 gctaaaggag gtgtcggcga tgtccgatga tatgctccgc gagatcctgg ccgatgccga
     4741 cgaccgcatg aaaaagacca tcgacgcgct gcgccgcgag ttgagcggca ttcggaccgg
     4801 gcgcgccgcg ccgggcctga tcgagcggct ggacgtcgac tactacggca cctcgacccc
     4861 gctcaaccaa ctggccggta tcagcgcccc cgaggcccgg ctgctggtcg tgcaaccgtg
     4921 ggaccgcggc agcatgggtg cgatcgagaa ggccatccgc tcctccgacc tgggcctcaa
     4981 tccgaccaac gacggccagc tcatccgcat cgccatcccc gcgctcaccg aggagcggcg
     5041 caagtcgctg gtgcgcgtgg tgcggcagaa ggtggaagag gacaaggtcg cactgcgcaa
     5101 cgtgcggcgc gacgcgatgg ccgatatcaa ggagctgcag aaggagaagc tgatcggcga
     5161 ggacgaggag cgccgcgccg agcacgagct gcaggaggtc accaaccgct tcatcggcga
     5221 ggccgacaag attgggaagc acaaagagga tgaggtcctc gaagtctgag cctgccgcct
     5281 cccagcccga tacctgtcca ccggccccgg cacgggtgcc tcgccatgtc gccatcgtga
     5341 tggacggcaa cggccgctgg gcaacccagc gcgggctgcc gcgcgtggcc gggcacgagg
     5401 ccggcacgga gaatatccgg cgcatcacgc gccgggcggc cgaacggggc atcgactatc
     5461 tcaccctctg gggcttctcc accgagaact ggcggcgtcc acaggacgag gtcgaagcca
     5521 tcctcggcat cctggctcgc tccgtctcca acgagaccga ggagctgcac cacgagggtg
     5581 tgcggctcaa gcacatcggg agcctggccc cgctcgatcc ctccctgcgc tacgccatcg
     5641 aggacgccat cgagctgacg cggaacaaca cccgggtcac cctcaccatc gccttcaact
     5701 acggcggccg cgccgagttg atccaggcca tccagcgcat cgtggccgat gggatcgccc
     5761 ccgagcagat cgacgaggcg ctgatctcgc gctacctcta cacccacgac atgcccgagc
     5821 ccgacctgat catccgcact tcgggcgagc tgcgcgtcag caatttcctc atctggcagg
     5881 gcgcctacgc cgaatacatc ttcacctcaa aactctggcc cgacttcggc cccgacgatc
     5941 tcgtctgcgc ggtcgaggag tacgcccggc gcgagcggcg cttcggcggc ctggggccat
     6001 cggcggcgcc ccccggcgcg gcctagccgg ggcggggccg tgctgcgcca gcgatcgatc
     6061 agcgcggtcg tcgtcgtgct cttcgccgcc atccccgccc tgctcggtgg cccgctcttc
     6121 gccgccgcca tggtcgtgct cgcgctcctg ggtacgcgcg agctgctgcg ggcgctcgac
     6181 ggccgtatcg atcgcgccac cgaggcgctc gtctacctgg cggccctcgg cctgatcctc
     6241 acggcggcgt tcgacggcgc ggggccgggg ctggcagccg tgatcgtact cttcgtgatc
     6301 gcgtcgctga tcgcgggcgt gctgcgcacc gcggtcgaga ccggactggc cgcctggagc
     6361 gccggcatcc tgaccatcgc ctacctcggg ctgccgctgg cctacgccgt cgccctgcgc
     6421 gatctccccg gcgcgtccga gcgtggctgg gtcaatcacg tcaccgaccc gctggccgcc
     6481 ggcaccggcg ccgggctcgc ctgggtcgcg ctggtgttcg ccgtgacctg gctcaccgac
     6541 accgcggcct acctggtcgg ccgtcgcttc ggccgcacca agctcgtccc ggcgctcagt
     6601 ccgggtaaga cgcgggcggg tgcgctcggt gggatcgtgg cgggcgcgct gagcgcgccg
     6661 ctcgccgcct gggtcttcgg cgcgccgctc gggctgccgc tggccgccgt tctgggcctg
     6721 atcctgtcgc tggcggggca actcggcgat ctgggggagt cgttgatcaa gcgcaacctc
     6781 ggtatcaaag atatggggac gctgatcccg gggcacggcg gtgtgctcga tcgcatcgac
     6841 gcgctcctct tcaccttccc gctg
//
